LOCUS       DAJTZL010000001        88078 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.001, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000001 DAJTZL010000000
VERSION     DAJTZL010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 88078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 88078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..88078
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.001"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..903)
                     /gene="nac"
                     /locus_tag="OO544_000001"
     CDS             complement(<1..903)
                     /gene="nac"
                     /locus_tag="OO544_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HCR6765715.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQALSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEIVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQSVDVNAIAKMNLFLPSDYSAIRLRVDEAFSLRRFTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQW"
     gene            1230..1305
                     /locus_tag="OO544_000002"
     tRNA            1230..1305
                     /locus_tag="OO544_000002"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1263..1265,aa:Asn,seq:gtt)
     gene            complement(1361..2296)
                     /gene="ldtA"
                     /locus_tag="OO544_000003"
     CDS             complement(1361..2296)
                     /gene="ldtA"
                     /locus_tag="OO544_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR6765716.1"
                     /translation="MMRRVNIFCSFALLFASHNSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKSGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSNWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFISGQEVDVNRANAALQRRSGMPVQISSGSRQMF"
     gene            complement(2358..3437)
                     /gene="cobT"
                     /locus_tag="OO544_000004"
     CDS             complement(2358..3437)
                     /gene="cobT"
                     /locus_tag="OO544_000004"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HCR6765717.1"
                     /translation="MQTLADLLNTIPAIDPAAMSRAQRHIDGLLKPVGSLGRLEALAI
                     QLAGMPGLNGVPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVYVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRHLAEKLLLDVICYT
                     RELAKNGVTLFGVGDLGMANTTPAAAIVSTITGRAPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGMAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMEMRLGEGSGAALAMPIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(3449..4192)
                     /gene="cobS"
                     /locus_tag="OO544_000005"
     CDS             complement(3449..4192)
                     /gene="cobS"
                     /locus_tag="OO544_000005"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HCR6765718.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGVPLAALFSVLVLALMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(4189..4734)
                     /gene="cobU"
                     /locus_tag="OO544_000006"
     CDS             complement(4189..4734)
                     /gene="cobU"
                     /locus_tag="OO544_000006"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HCR6765719.1"
                     /translation="MMILVTGGARSGKSRHAEALIGDSSQVLYIATSQILDDEMAARI
                     EHHRQGRPAHWHTVERWQHLDELIHADINPHEAVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            complement(4868..5305)
                     /locus_tag="OO544_000007"
     CDS             complement(4868..5305)
                     /locus_tag="OO544_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HCR6765720.1"
                     /translation="MKTIKPESIALFCLLSGDVDLAKHLAEILPITCFVGEDWQQDFQ
                     PLDGGPVQSLSDAYSDYSVLLLIAAESEISVPEWLAHSEGTTVILIAEHNARLLTGPA
                     GGAVALIHYLNEICHSRETAVNDAFCRTLTRGSLAIPQCHLLS"
     regulatory      complement(5523..5698)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(5787..6463)
                     /locus_tag="OO544_000008"
                     /pseudo
     CDS             complement(5787..6463)
                     /locus_tag="OO544_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460981.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PocR ligand-binding domain-containing protein"
     gene            complement(6666..7475)
                     /gene="pduF"
                     /locus_tag="OO544_000009"
     CDS             complement(6666..7475)
                     /gene="pduF"
                     /locus_tag="OO544_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol diffusion facilitator PduF"
                     /protein_id="HCR6765721.1"
                     /translation="MNDSLKAQCVAEFLGTGLFLFFGIGCLCALKVAGASLGLWEICI
                     IWGLGISLAVYLTAGISGAHLNPAVTIALWLFASFPARKVLPFCLAQLAGAFGGAVLA
                     YSLYSSLFTDFESAHNMVRGSAESLQLASIFSTYPAAAINVWQAALVEVVITSMLMGL
                     IMALTDDGNGVPKGPLAPLLIGILVAVIGASTGPLTGFAMNPARDFGPKIFTWLAGWG
                     EIAMTGGRNIPYFIVPIIAPIIGACVGAAIYRYLIAKNLPVNSVTPKGISE"
     gene            7987..8271
                     /gene="pduA"
                     /locus_tag="OO544_000010"
     CDS             7987..8271
                     /gene="pduA"
                     /locus_tag="OO544_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduA"
                     /protein_id="HCR6765722.1"
                     /translation="MQQEALGMVETKGLTAAIEAADAMVKSANVLLVGYEKIGSGLVT
                     VIVRGDVGAVKAATDAGAAAAHNVGEVKAVHVIPRPHTDVEKILPKGINP"
     gene            8268..9080
                     /gene="pduB"
                     /locus_tag="OO544_000011"
     CDS             8268..9080
                     /gene="pduB"
                     /locus_tag="OO544_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduB"
                     /protein_id="HCR6765723.1"
                     /translation="MSSNELVEQIMAQVIARVATPEHEVLPKTTHQIRETAMAEKSCT
                     LTEFVGTAIGDTVGLVIANVDSALLDAMKLEKRYRSIGILGARIGAGPHIMAADEAVK
                     ATNTEVISIELPRDTKGGAGHGSLILLGGNDVSDVKRGIEVALKELDRTFGDVYANEA
                     GHLELQYTARASYALEKAFGAPLGRACGVIVGAPAAVGVLMADTALKSANVDVVAYSS
                     PAQDTSYSNEVILIISGDSGAVRQAVISAREIGKTVLSTLGDTPKNDHPSYI"
     gene            9099..10763
                     /gene="pduC"
                     /locus_tag="OO544_000012"
     CDS             9099..10763
                     /gene="pduC"
                     /locus_tag="OO544_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol dehydratase large subunit PduC"
                     /protein_id="HCR6765724.1"
                     /translation="MRSKRFEALAKRPVNQDGFVKEWIEEGFIAMESPNDPKPSIKIV
                     NGVVTELDGKPASQFDLIDHFIARYGINLARAEEVIAMDSVKLANMLCDPNVKRSDIV
                     PLTTAMTPAKIVEVVSQMNVVEMMMAMQKMRARRTPSQQAHVTNVKDNPVQIAADAAE
                     GAWRGFDEQETTVAVARYAPFNAIALLVGSQVGRPGVLTQCSLEEATELKLGMLGHTC
                     YAETISVYGTEPVFTNGDDTPWSKGFLASSYASRGLKMRFTSGSGSEVQMGYAEGKSM
                     LYLEARCIYITKAAGVQGLQNGSVSCIGVPSAVPSGIRAVLAENLICSSLDLECASSN
                     DQTFTHSDMRRTARFLMQFLPGTDFISSGFSAVPNYDNMFAGSNEDAEDFDDYNVIQR
                     DLKVDGGLRPVREEDVIAIRNKAARALQAVFAGMGLPPITDEEVEAATYAHGSKDMPE
                     RNIVEDIKFAQEIINKNRNSLEVVKALAQGSFTDVAQDMLNMQKAKLTGDYLHTSAII
                     VDDGQVLSAVNDVNDYAGPATGYRLQGERWEEIKNIPGALDPNEID"
     gene            10773..11441
                     /locus_tag="OO544_000013"
     CDS             10773..11441
                     /locus_tag="OO544_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol/glycerol family dehydratase medium
                     subunit"
                     /protein_id="HCR6765725.1"
                     /translation="MEINEKLLRQIIEDVLAEMQPSDKSVSFRAPVSATVPSAPDTGN
                     FLTEIGEAQQGTQQDEVIIAVGPAFGLAQTVNIIGIPHKNILREVIAGIEEEGIKARV
                     IRCFKSSDVAFVAVEGDRLSGSGIAIGIQSKGTTVIHQQGLPPLSNLELFPQAPLLTL
                     DTYRQIGKNAARYAKRESPQPVPTLNDQMARPKYQAKSAILHIKETKYVVTGKKPQEL
                     RVTF"
     gene            11456..11974
                     /gene="pduE"
                     /locus_tag="OO544_000014"
     CDS             11456..11974
                     /gene="pduE"
                     /locus_tag="OO544_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol dehydratase small subunit PduE"
                     /protein_id="HCR6765726.1"
                     /translation="MNTDAIESMVRDVLNRMNSLQDAAPVSAVPNASILSAKVTDYPL
                     ANKHPEWVKTATNKTLDDFTLENVLSDNVTALDMRITPETLRIQAAIARDAGRDRLAM
                     NFERAAELTSVPDDRILEIYNALRPYRSTKQELIAIADDLEQRYQAKICAAFVREAAE
                     LYVERKKLKGDD"
     gene            11984..13816
                     /locus_tag="OO544_000015"
     CDS             11984..13816
                     /locus_tag="OO544_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diol dehydratase reactivase subunit alpha"
                     /protein_id="HCR6765727.1"
                     /translation="MRYIAGIDIGNSSTEVALATLSATGELSFVSSALEETTGIKGTL
                     RNVHGIQEALAQATKKVGINVSDISLIRINEATPVIGDVAMETITETIITESTMIGHN
                     PKTPGGVGLGVGFTITPQELLTRPADTPYILVVSSAFDFADIATMINASVRAGYQLTG
                     VILQRDDGVLVNNRLEIPLPIVDEVLYIDRIPLGMLAAIEVAVPGKVIETLSNPYGIA
                     TVFALNAEETKNIVPVARALIGNRSAVVVKTPSGDVKARSIPAGNIELLSAGRTTRVD
                     VAAGADAIMKAVGECPKLENVTGEPGTNIGGMLEHVRQTMAELTNKPSNEIFIQDLLA
                     IDTSVPVSVTGGLAGEFSLEQAVGIASMVKSDRLQMAMIASEIKQKLHVDVQVGGAEA
                     EAAIQGALTTPGTTRPLAILDLGAGSTDASIINQSGEIVATHLAGAGDMVTMIIAREL
                     GLNDRYLAEEIKKYPLAKVESLFHLRHEDGSVQFFPTPLSPHVFARVCVVKPDELVPI
                     PGDLTLEKVRAVRRSAKERVFVTNALRALRQVSPAGNIRDIPFVVLVGGSSLDFEVPQ
                     LVTDALAHYRLVAGRGNIRGSEGPRNAVATGLLIAWHKESIHGK"
     gene            13806..14156
                     /locus_tag="OO544_000016"
     CDS             13806..14156
                     /locus_tag="OO544_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydratase reactivase beta/small
                     subunit family protein"
                     /protein_id="HCR6765728.1"
                     /translation="MESKERAPAIVVMEIGDCITTWDEVLLGIEEEGIPFRIQHIPSG
                     EVIDSAWLAARQSPLLVGIACDREELIVHYKNLPASAPLFTLTYQQDNHTRRSIGTNA
                     ARLVKGIPFREFHS"
     gene            14176..14451
                     /gene="pduJ"
                     /locus_tag="OO544_000017"
     CDS             14176..14451
                     /gene="pduJ"
                     /locus_tag="OO544_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduJ"
                     /protein_id="HCR6765729.1"
                     /translation="MNNALGLVETKGLVGAIEAADAMVKSANVQLVGYEKIGSGLITV
                     MVRGDVGAVKAAVDAGSAAASAVGEVKSCHVIPRPHSDVEAILPKSA"
     gene            14475..14897
                     /locus_tag="OO544_000018"
     CDS             14475..14897
                     /locus_tag="OO544_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001607636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HCR6765730.1"
                     /translation="MKQSLGLLEASGLALAISCADAMAKAASITLVGLEKTNGSGWTL
                     IKITGDVASVNAAIATGASFAGQRNGLVAHKVIARPGDGILPHTTEALTVTVETSKTP
                     ATTVTSEIISCNLCLDPACQRQKGEPRSLCLYANKRGE"
     gene            14900..15532
                     /locus_tag="OO544_000019"
     CDS             14900..15532
                     /locus_tag="OO544_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HCR6765731.1"
                     /translation="MDKQTLQETVSQILDEMRERPIPLGVSSRHIHLSSADYLRLFPE
                     QPISEKKPLMQPGQYAAEQVVTLEGPKGKLHNVRLLGPLRNASQVEISRTDARTLGIT
                     APLRMSGDLSGTPGIRLISPFAELEITSGVIVAQRHIHMSPLDALIYGVTHGDKVAVA
                     IEGDERRLIFENVAVRVSPDMRLEMHIDTDEANAAGADSPDVFATLVSVP"
     gene            15529..16020
                     /gene="pduM"
                     /locus_tag="OO544_000020"
     CDS             15529..16020
                     /gene="pduM"
                     /locus_tag="OO544_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcompartment protein PduM"
                     /protein_id="HCR6765732.1"
                     /translation="MNNMLMQRIVDEVVFRLKQRAGKTLVLTVFQLRDASVQESVHQY
                     ASLQIRYVDLPLLRQLAENETSDRASIQIHEALAWGMHIQLSLQRHFLNAIELKTLAR
                     LPLSWCDEQGQPIYLHHNRLLSYADIAQLSSGILVLQRKCCVTALAREAAITRNIQLI
                     RQE"
     gene            16024..16299
                     /gene="pduN"
                     /locus_tag="OO544_000021"
     CDS             16024..16299
                     /gene="pduN"
                     /locus_tag="OO544_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduN"
                     /protein_id="HCR6765733.1"
                     /translation="MHLARVTGAVVSTQKSPSLIGKKLLLVRRVSADGELPLTPLTGD
                     EVAVDSVGAGVGELVLLSSGSSARHVFSGPNEAIDLAVVGIVDTLSR"
     gene            16311..17318
                     /gene="pduO"
                     /locus_tag="OO544_000022"
     CDS             16311..17318
                     /gene="pduO"
                     /locus_tag="OO544_000022"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-domain cob(I)yrinic acid a,c-diamide
                     adenosyltransferase PduO"
                     /protein_id="HCR6765734.1"
                     /translation="MAIYTRTGDSGSTSLFTGQRVSKTHLRVETYGTLDELNATLSLC
                     YCATAIESHRILLEAIQQQIFWFSAELASESEQPSAQQRYIGTEEIAALENAIDSAMN
                     AVPPVHSFILPGRCEAASRMHFARTVARRAERRLVELTTETTVRNVLLHYINRLSDCL
                     YALARVEDNVAHQNLMIQEITKRYHEANHTPALKERTMPLTFQDLHQLIRSAAMRADE
                     LHIPVVISIVDANGTESVTWRMPDALLVSSELAPKKAWTAVAMKTATHKLADTVQPDA
                     PLYGLESHMQGKVVTFGGGFPLWRDGKLLGGLGISGGSVEQDMDIAQSAMAAINVGVN
                     Q"
     gene            17315..18700
                     /gene="pduP"
                     /locus_tag="OO544_000023"
                     /pseudo
     CDS             17315..18700
                     /gene="pduP"
                     /locus_tag="OO544_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460996.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating propionaldehyde dehydrogenase
                     PduP"
     gene            18711..19823
                     /locus_tag="OO544_000024"
     CDS             18711..19823
                     /locus_tag="OO544_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001091448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HCR6765735.1"
                     /translation="MNTFSLQTRLYSGPGSLKVLSRFSHRHIWIICDAFLARSPLIDT
                     LHQALPDSNRLSIFSEITPAPTIQTVVKGIAQMQTLRPDVVIGFGGGSALDAAKAIVW
                     FGRQCGIEIETCVAIPTTSGIGSEVTSACVISDPEKGIKYPLFDNALYPDIAILDPSL
                     VVSVPPAITANTGMDVLTHALEAYVSPRASDFTDALVEKAVQIVFQYLPTAVKKGDCL
                     ATRGKMHNASTLAGMAFSQAGLGLNHAIAHQLGGQFHLPHGLANALLLTAVIRFNAGD
                     PRTAKRYARLAKTCHLCPDNANDTASLNALIQHIEQLNTTCTLPTLANALKEKKAEWS
                     IRIPDMVQAALADATLRTNPRAADASAIAELLKGLL"
     gene            19820..21165
                     /locus_tag="OO544_000025"
                     /pseudo
     CDS             19820..21165
                     /locus_tag="OO544_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585845.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
     gene            21168..21722
                     /gene="pduT"
                     /locus_tag="OO544_000026"
     CDS             21168..21722
                     /gene="pduT"
                     /locus_tag="OO544_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduT"
                     /protein_id="HCR6765736.1"
                     /translation="MSKAVGILELSSIAKGMETGDAMLKSANVELLVSKTLCPGKFLL
                     MLGGDVGAVQQAINSGASLAGEMLVDSLVLPNIHPSLLSAISGLNHVEQHRAVGVVET
                     WSVAACIDAADCAVKAANVILVRVHMAFGIGGKCYMVVAGDISDVNNAVSAASVRAGE
                     KGLLVYRSVIARPHEAMWRQMVEG"
     gene            21722..22072
                     /gene="pduU"
                     /locus_tag="OO544_000027"
     CDS             21722..22072
                     /gene="pduU"
                     /locus_tag="OO544_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000441103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduU"
                     /protein_id="HCR6765737.1"
                     /translation="MTTQHTTERMIQEYVPGKQVTLAHVIANPGKDLFKKLGLPDTIS
                     AIGILTITPSEASIIACDIATKSGAVEIGFLDRFTGAVVLTGDVSAIEYALSQVTRTL
                     GEMMRFTACPITRT"
     gene            22077..22520
                     /locus_tag="OO544_000028"
     CDS             22077..22520
                     /locus_tag="OO544_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HCR6765738.1"
                     /translation="MQRMMLIGPSQCGKTSLTQCLRGENIHYQKTQAIVWSPTTIDTP
                     GEYLENRSLYSALLVSACEADIIALVLNADAPWSPFSPGFTSSMNRPVIGVVTKADLA
                     NMEQISLVKCWLREAGAHNVLVTSAVNNHGVAELLALLHTEEGCR"
     gene            complement(22621..22950)
                     /locus_tag="OO544_000029"
     CDS             complement(22621..22950)
                     /locus_tag="OO544_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HCR6765739.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(23122..24180)
                     /locus_tag="OO544_000030"
     CDS             complement(23122..24180)
                     /locus_tag="OO544_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCR6765740.1"
                     /translation="MRADKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     STWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEIDTALWRSGDVILGSLLAMLFTG
                     IWPQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIA
                     PASKETRIPKSIYEGIQTINRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQ
                     QILLSFVHALYEGNPQPVFANTEKLNDAVEELRQLLNNHHDLKVVETPIYGYVWLNME
                     TAHQLELLSNLICRALRK"
     gene            complement(24378..24851)
                     /gene="sbmC"
                     /locus_tag="OO544_000031"
     CDS             complement(24378..24851)
                     /gene="sbmC"
                     /locus_tag="OO544_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HCR6765741.1"
                     /translation="MNYEITQEEKRTVAGFHLVGPWEQTVKKGFEQLMMWVDNKNIVP
                     KEWVAVYYDNPDETPAEKLRCDTVVTVPNNFTLPENSEGVILTEISGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPKPCFEVYLNNGAEDGYWDIEMYVAVQPKHH"
     gene            complement(24970..26136)
                     /gene="dacD"
                     /locus_tag="OO544_000032"
     CDS             complement(24970..26136)
                     /gene="dacD"
                     /locus_tag="OO544_000032"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HCR6765742.1"
                     /translation="MKRRLIIAASLFVFNLSSGFAAENIPFSPQPPEIHAGSWVLMDY
                     TTGQILTAGNEHQQRNPASLTKLMTGYVVDRAIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVEMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVFSRAIIHGEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNVDGLKTGHTSGAGFNLIASAVDGQRRLIAVVMGADSAKGREEEARKLLR
                     WGQQNFTTVQILHRGKKVGTERIWYGDKENIDLGTEQEFWMVLPKAEIPHIKAKYTLD
                     GKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGGMFSRLSDYFHHKA"
     gene            26345..27772
                     /gene="sbcB"
                     /locus_tag="OO544_000033"
     CDS             26345..27772
                     /gene="sbcB"
                     /locus_tag="OO544_000033"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HCR6765743.1"
                     /translation="MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDNEFNVIG
                     EPEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGY
                     NNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
                     LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLM
                     ALIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDS
                     DTLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNL
                     KILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLP
                     ALDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQML
                     AQQYADNKEKVALLKALWQYAEEIV"
     gene            complement(27815..28042)
                     /locus_tag="OO544_000034"
     CDS             complement(27815..28042)
                     /locus_tag="OO544_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HCR6765744.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(28056..29114)
                     /gene="yeeE"
                     /locus_tag="OO544_000035"
     CDS             complement(28056..29114)
                     /gene="yeeE"
                     /locus_tag="OO544_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transport protein YeeE"
                     /protein_id="HCR6765745.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISVQSVGVFALIQAGLLTYEAEAFPWLGTVIGGYIFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYSTEHNSIAETFNLSVWPLVAVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGITSPTANILQFLVAGDVKYINWGVFLVLGIFVGSFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCFIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            complement(29293..30651)
                     /gene="plaP"
                     /locus_tag="OO544_000036"
     CDS             complement(29293..30651)
                     /gene="plaP"
                     /locus_tag="OO544_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HCR6765746.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(30641..30703)
                     /gene="yoeI"
                     /locus_tag="OO544_000037"
     CDS             complement(30641..30703)
                     /gene="yoeI"
                     /locus_tag="OO544_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HCR6765747.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(30918..31847)
                     /locus_tag="OO544_000038"
     CDS             complement(30918..31847)
                     /locus_tag="OO544_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6765748.1"
                     /translation="MKQLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLVQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(31893..32717)
                     /locus_tag="OO544_000039"
     CDS             complement(31893..32717)
                     /locus_tag="OO544_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR6765749.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(32800..33054)
                     /gene="yoeB"
                     /locus_tag="OO544_000040"
     CDS             complement(32800..33054)
                     /gene="yoeB"
                     /locus_tag="OO544_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HCR6765750.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
     gene            complement(33051..33302)
                     /gene="yefM"
                     /locus_tag="OO544_000041"
     CDS             complement(33051..33302)
                     /gene="yefM"
                     /locus_tag="OO544_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HCR6765751.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDIIE"
     gene            33585..33635
                     /gene="hisL"
                     /locus_tag="OO544_000042"
     CDS             33585..33635
                     /gene="hisL"
                     /locus_tag="OO544_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HCR6765752.1"
                     /translation="MTRVQFKHHHHHHHPD"
     misc_feature    33611..33734
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            33781..34680
                     /gene="hisG"
                     /locus_tag="OO544_000043"
     CDS             33781..34680
                     /gene="hisG"
                     /locus_tag="OO544_000043"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HCR6765753.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            34686..35990
                     /gene="hisD"
                     /locus_tag="OO544_000044"
     CDS             34686..35990
                     /gene="hisD"
                     /locus_tag="OO544_000044"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HCR6765754.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     ARGDDALREYSAKFDKTTVNALKVSAEEIAAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVKDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MAHQVAEAVERQLAELPRAETARQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARDLVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNALKEQA"
     gene            35987..37057
                     /gene="hisC"
                     /locus_tag="OO544_000045"
     CDS             35987..37057
                     /gene="hisC"
                     /locus_tag="OO544_000045"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HCR6765755.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTQDNWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLTEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIVAMRERVTQIIAER
                     EYLIAALKEIPCVEQVFDSATNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            37057..38124
                     /gene="hisB"
                     /locus_tag="OO544_000046"
     CDS             37057..38124
                     /gene="hisB"
                     /locus_tag="OO544_000046"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HCR6765756.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDREILNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKMALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            38124..38714
                     /gene="hisH"
                     /locus_tag="OO544_000047"
     CDS             38124..38714
                     /gene="hisH"
                     /locus_tag="OO544_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HCR6765757.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            38714..39451
                     /gene="hisA"
                     /locus_tag="OO544_000048"
     CDS             38714..39451
                     /gene="hisA"
                     /locus_tag="OO544_000048"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HCR6765758.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPERVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            39433..40209
                     /gene="hisF"
                     /locus_tag="OO544_000049"
     CDS             39433..40209
                     /gene="hisF"
                     /locus_tag="OO544_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HCR6765759.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDGETGKYHVNQYTGDESRTRVTQWETL
                     DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            40203..40814
                     /locus_tag="OO544_000050"
     CDS             40203..40814
                     /locus_tag="OO544_000050"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HCR6765760.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVNIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
     gene            complement(40911..41888)
                     /gene="wzzB"
                     /locus_tag="OO544_000051"
     CDS             complement(40911..41888)
                     /gene="wzzB"
                     /locus_tag="OO544_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HCR6765761.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVAKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIENLKVDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(42034..43200)
                     /gene="ugd"
                     /locus_tag="OO544_000052"
     CDS             complement(42034..43200)
                     /gene="ugd"
                     /locus_tag="OO544_000052"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HCR6765762.1"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQQFLQSDKIHFNATLDKNEAYRDADYVIIATPTDYDPKTNYFNTSSVESVIK
                     DVVEINPYAVMVIKSTVPVGFTEAMHKKYRTENIIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLAIFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
     gene            complement(43450..44856)
                     /gene="gndA"
                     /locus_tag="OO544_000053"
     CDS             complement(43450..44856)
                     /gene="gndA"
                     /locus_tag="OO544_000053"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HCR6765763.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQSAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(45020..46390)
                     /gene="cpsG"
                     /locus_tag="OO544_000054"
                     /gene_synonym="manB"
     CDS             complement(45020..46390)
                     /gene="cpsG"
                     /locus_tag="OO544_000054"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HCR6765764.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALARGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPFEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRTSNTEPVVRLNVESRGDVPLMEEKTKLI
                     LELLNK"
     gene            complement(46495..47931)
                     /gene="cpsB"
                     /locus_tag="OO544_000055"
     CDS             complement(46495..47931)
                     /gene="cpsB"
                     /locus_tag="OO544_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HCR6765765.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGAMHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            complement(47934..49157)
                     /gene="wcaI"
                     /locus_tag="OO544_000056"
     CDS             complement(47934..49157)
                     /gene="wcaI"
                     /locus_tag="OO544_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HCR6765766.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGSKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAAENVIFFPNWSEIARFQHVADADV
                     DALRNQLDLPDNKKIILYSGNIGEKQGLENVIEAADRLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            complement(49154..49633)
                     /gene="gmm"
                     /locus_tag="OO544_000057"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             complement(49154..49633)
                     /gene="gmm"
                     /locus_tag="OO544_000057"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HCR6765767.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELILPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            complement(49636..50601)
                     /gene="fcl"
                     /locus_tag="OO544_000058"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             complement(49636..50601)
                     /gene="fcl"
                     /locus_tag="OO544_000058"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HCR6765768.1"
                     /translation="MSKQRIFIAGHRGMVGSAIRRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRG"
     gene            complement(50604..51725)
                     /gene="gmd"
                     /locus_tag="OO544_000059"
     CDS             complement(50604..51725)
                     /gene="gmd"
                     /locus_tag="OO544_000059"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HCR6765769.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            complement(51751..52299)
                     /gene="wcaF"
                     /locus_tag="OO544_000060"
     CDS             complement(51751..52299)
                     /gene="wcaF"
                     /locus_tag="OO544_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HCR6765770.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            complement(52315..53061)
                     /gene="wcaE"
                     /locus_tag="OO544_000061"
     CDS             complement(52315..53061)
                     /gene="wcaE"
                     /locus_tag="OO544_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HCR6765771.1"
                     /translation="MLLSIITVAFRNLEGIVKTHASLAHLAQAEDISFEWIVVDGGSN
                     DGTREYLENLNGIYNLRFVSEPDNGIYDAMNKGITMAQGKFALFLNSGDIFHQDAANF
                     VRKLKMQKDNMMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWAELSWHLRQRTTSKTKALYNKV"
     gene            complement(53072..54289)
                     /gene="wcaD"
                     /locus_tag="OO544_000062"
     CDS             complement(53072..54289)
                     /gene="wcaD"
                     /locus_tag="OO544_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HCR6765772.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGIGAGLTAFNYLFGQSLDASKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVIGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFRVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNITR"
     gene            complement(54264..55481)
                     /gene="wcaC"
                     /locus_tag="OO544_000063"
     CDS             complement(54264..55481)
                     /gene="wcaC"
                     /locus_tag="OO544_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HCR6765773.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLKSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVDEEDVLQLVQLSKPEIAQAIFGTTLAEFSQRSRATYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(55478..55966)
                     /gene="wcaB"
                     /locus_tag="OO544_000064"
     CDS             complement(55478..55966)
                     /gene="wcaB"
                     /locus_tag="OO544_000064"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HCR6765774.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(55969..56808)
                     /gene="wcaA"
                     /locus_tag="OO544_000065"
     CDS             complement(55969..56808)
                     /gene="wcaA"
                     /locus_tag="OO544_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HCR6765775.1"
                     /translation="MKENPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            complement(56901..59063)
                     /gene="wzc"
                     /locus_tag="OO544_000066"
     CDS             complement(56901..59063)
                     /gene="wzc"
                     /locus_tag="OO544_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HCR6765776.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWERLMGRQNETVKVTTFNRPKEMAGQVFTLNVLDNK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEEEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGIKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVSWASKNYDLVLIDTPPILAVTDAAIVGRHAGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(59066..59509)
                     /gene="wzb"
                     /locus_tag="OO544_000067"
     CDS             complement(59066..59509)
                     /gene="wzb"
                     /locus_tag="OO544_000067"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HCR6765777.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(59515..60654)
                     /gene="wza"
                     /locus_tag="OO544_000068"
     CDS             complement(59515..60654)
                     /gene="wza"
                     /locus_tag="OO544_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HCR6765778.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            60963..61112
                     /locus_tag="OO544_000069"
     CDS             60963..61112
                     /locus_tag="OO544_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6765779.1"
                     /translation="MVIEYWLSNNAGGSVTANGIAGKVTIRQLAIFKNYIKCHSIWFL
                     GVSLG"
     gene            61313..62896
                     /locus_tag="OO544_000070"
     CDS             61313..62896
                     /locus_tag="OO544_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCR6765780.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPRQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIVGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLHKDGELEYEV"
     gene            complement(63158..65023)
                     /gene="asmA"
                     /locus_tag="OO544_000071"
     CDS             complement(63158..65023)
                     /gene="asmA"
                     /locus_tag="OO544_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HCR6765781.1"
                     /translation="MRRFLTTLMILLVVLVAGLSVLVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQAQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKMLVTR"
     gene            complement(65045..65626)
                     /gene="dcd"
                     /locus_tag="OO544_000072"
     CDS             complement(65045..65626)
                     /gene="dcd"
                     /locus_tag="OO544_000072"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HCR6765782.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(65718..66359)
                     /gene="udk"
                     /locus_tag="OO544_000073"
     CDS             complement(65718..66359)
                     /gene="udk"
                     /locus_tag="OO544_000073"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HCR6765783.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            66677..69994
                     /locus_tag="OO544_000074"
     CDS             66677..69994
                     /locus_tag="OO544_000074"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCR6765784.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLGLVSFIFTLFSLELSQFGTQLA
                     PLWFPTSIMMVAFYRHAGRMWPGIALSCSLGNIAASILLFSTSSLNMTWTTINIVEAV
                     VGAVLLRKLLPWYNPLQNLADWLRLALGSAIVPPLLGGVLVVLLTPGDDPLRAFLIWV
                     LSESIGALALVPLGLLFKPHYLLRHRNPRLLFESLLTLAITLTLSWLSMLYLPWPFTF
                     IIVLLMWSAVRLPRMEAFLIFLTTVMMVSLMMAADPSLLATPRTYLMSHMPWLPFLLI
                     LLPANIMTMVMYAFRAERKHISESETRFRNAMEYSAIGMALVGTEGQWLQTNKALCQF
                     LGYSQEELRGLTFQQLTWPEDLNKDLQQVEKLISGEINTYSMEKRYYNRNGDVVWALL
                     AVSLVRHTDGTPLYFIAQIEDINELKRTEQVNQQLMERITLANEAGGIGIWERELKPN
                     VFSWDKRMFELYEIPPHIKPNWQVWYECVLPEDRQHAEKVIRDSLQSRSPFKLEFRIT
                     VKDGIRHIRALANRVLNKEGEVERLLGINMDMTEVKQLNEALFQEKERLHITLDSIGE
                     AVVCIDMAMKITFMNPVAEKMSGWTQEEALGVPLLTVLHITFGDNGPLMENIYSADTS
                     RSAIEQDVVLHCRSGGSYDVHYSITPLSTLDGSNIGSVLVIQDVTESRKMLRQLSYSA
                     SHDALTHLANRASFEKQLRILLQTVNSTHQRHALVFIDLDRFKAVNDSAGHAAGDALL
                     RELASLMLSMLRSSDVLARLGGDEFGLLLPDCNVESARFIATRIISAVNDYHFIWEGR
                     VHRVGASAGITLIDDNNHQAAEVMSQADIACYASKNGGRGRVTVYEPQQAAAHSERAA
                     MSLDEQWRMIKENQLMMIAHGVASPRIPEARNLWLISLKLWSCEGEIIDEQTFRRSFS
                     DPALSHALDRRVFHEFFQQAAKAVASKGISIALPLSVAGLSSATLVNDLLEQLENSPL
                     PPRLLHLIIPAEAILDHAESVQKLQLAGCRIVLSQVGRDLQIFNSLKANMADYLLLDG
                     ELCANVQGNLMDEMLITIIQGHAQRLGMKTIAGPVVLPLVMDTLSGIGVDLIYGDVIA
                     DAQPLDLLVNSSYFAIN"
     gene            complement(70104..70952)
                     /gene="alkA"
                     /locus_tag="OO544_000075"
     CDS             complement(70104..70952)
                     /gene="alkA"
                     /locus_tag="OO544_000075"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HCR6765785.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVSGVETVADNYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLLDLQCNPQIVNGALGKLGAGR
                     PGLRLPGCIDAFEQGVRAILGQLVSVAMAAKLTAKVVQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGSLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKRRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDEA"
     gene            71086..72438
                     /gene="yegD"
                     /locus_tag="OO544_000076"
     CDS             71086..72438
                     /gene="yegD"
                     /locus_tag="OO544_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCR6765786.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPQLLKMENDSTLLPSMLCAPTRE
                     SVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQEILERAAKRAGFRDVVFQYEPIAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEECKIALSSVAETRASLPFISNELATLISQRGLESALSQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPQIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(72451..74391)
                     /gene="yegI"
                     /locus_tag="OO544_000077"
     CDS             complement(72451..74391)
                     /gene="yegI"
                     /locus_tag="OO544_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HCR6765787.1"
                     /translation="MKPTLYTATGECVTPGRELGKGGEGAVYDINEFVDSVAKIYHTP
                     PPALKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYS
                     PAHRRQRYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLIDS
                     DSFQINANGTLHLCEVGVSHFTPPELQTLSSFVGFERTENHDNFGLALLIFHVLFGGR
                     HPYSGVPLISDAGNALETDITHFRYAYASDNQRRGLKPPPRSIPLSMLPSDVEAMFQQ
                     AFTESGVATGRPTAKAWVSALDSLRQQLKKCTVSAMHVYPGHLADCPWCALDNQGVIY
                     FIDLGEEVITTGGDFVLAKVWAMVMASVAPPALQLPLPDHFQPTGRPLPLGLLRREYI
                     ILLEIALSALSLLLCGLQAEPRYIILVPVLAAIWIIGSLTSKAYKAEVQQRREAFNRA
                     KMDYDHLVRQIQQVGGLEGFIAKRTMLEKMKDEILGLPEEEKRALAALHDTARERQKQ
                     KFLEGFFIDVASIPGVGPARKAALRSFGIETAADVTRRGVKQVKGFGDHLTQAVIDWK
                     ANCERRFVFRPNEAITPADRQAVMAKMTAKRHRLESALTVGATELQRFRLHAPARTMP
                     LMEPLRQAAEKLAQAQADLSRC"
     gene            complement(74388..75149)
                     /gene="pphC"
                     /locus_tag="OO544_000078"
     CDS             complement(74388..75149)
                     /gene="pphC"
                     /locus_tag="OO544_000078"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase PphC"
                     /protein_id="HCR6765788.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVVWLNDQQPLLVMFL
                     ADGAGSVSQGGEGATLAVNEAMAFMVQKVQDGELGLNDVLATNMVLTIRQRLFAEAEA
                     KALAVRDFACTFLGLISSPDGTLIMQIGDGGVVVDLGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLETFTSTGRAHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            complement(75146..75805)
                     /locus_tag="OO544_000079"
     CDS             complement(75146..75805)
                     /locus_tag="OO544_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HCR6765789.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMSGRPINELNAGL
                     VTFRDELLADPLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFQGEEDKKFAFFSIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            complement(76026..76085)
                     /gene="ibsA"
                     /locus_tag="OO544_000080"
     CDS             complement(76026..76085)
                     /gene="ibsA"
                     /locus_tag="OO544_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HCR6765790.1"
                     /translation="MMKHVIILVILLVISFQAY"
     gene            complement(76358..76414)
                     /locus_tag="OO544_000081"
     CDS             complement(76358..76414)
                     /locus_tag="OO544_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCR6765791.1"
                     /translation="MMKLLIIVVLLVISFPAY"
     gene            complement(76685..76741)
                     /locus_tag="OO544_000082"
     CDS             complement(76685..76741)
                     /locus_tag="OO544_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCR6765792.1"
                     /translation="MMKLLIIVVLLVISWPAY"
     gene            77020..78261
                     /gene="mdtA"
                     /locus_tag="OO544_000083"
     CDS             77020..78261
                     /gene="mdtA"
                     /locus_tag="OO544_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HCR6765793.1"
                     /translation="MKGSYKSRWVIVVVIAAIAAFWFWQGRNDSQSAAPGATKQAQQS
                     PAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTIIAANTVTVRSRVDGQLMALHFQ
                     EGQQVKAGDLLAEIDPSQFKVALAQTQGQLAKDKATLANARRDLARYQQLAKTNLVSR
                     QELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQISSG
                     DTTGIVVITQTHPIDLVFTLPESDIATVVQAQKAGKTLVVEAWDRTNSKKLSEGTLLS
                     LDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNEGH
                     FVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVEAQ
                     SATTPEEKATSREYAKKGARS"
     gene            78261..81383
                     /gene="mdtB"
                     /locus_tag="OO544_000084"
     CDS             78261..81383
                     /gene="mdtB"
                     /locus_tag="OO544_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HCR6765794.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFTNMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDQGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTEITPGLAALDTIRLTSSDGGVVPLSSIAKV
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQEIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPRDAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            81384..84461
                     /gene="mdtC"
                     /locus_tag="OO544_000085"
     CDS             81384..84461
                     /gene="mdtC"
                     /locus_tag="OO544_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HCR6765795.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVSVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRHLLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            84462..85877
                     /locus_tag="OO544_000086"
     CDS             84462..85877
                     /locus_tag="OO544_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6765796.1"
                     /translation="MTELPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLLLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLITLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            85874..87277
                     /gene="baeS"
                     /locus_tag="OO544_000087"
     CDS             85874..87277
                     /gene="baeS"
                     /locus_tag="OO544_000087"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HCR6765797.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDEQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            87274..87996
                     /gene="baeR"
                     /locus_tag="OO544_000088"
     CDS             87274..87996
                     /gene="baeR"
                     /locus_tag="OO544_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HCR6765798.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLPYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
BASE COUNT        21561 a        23316 c        22147 g        21054 t
ORIGIN      
        1 ccactgcctt ttttccatca ctggggaact aatcactgac attaacaact ctttcactgc
       61 ctgtgcctgt ggcgataagt tcgctctggc gggtaaattt aatgacaaag agagactcat
      121 ggaaggagtg gtaatgcgtg acatccaccc atttactgcg ccacataacg aacgcgcggc
      181 cgattcgggt aatactgcaa cgcccatgcc gctggcaatc gctgcggtaa gcgtggcaat
      241 agactcaatt tcaccaatga cttttgccgt gaatcgccgt aaggaaaaag cctcatcaac
      301 acgaagtcta atagcactgt aatcactggg gagaaagagg ttcattttcg caatagcatt
      361 cacatcaacg ctttgccccg ggcaatcttg agttcctacc agaaaaagat cttctttcag
      421 caaagcctga ctggatacac cagccacagg ggaatgctca taaatcaccg ccatatcgag
      481 ttggtgattt atcaattttt cgttaagcac tgcaccacta ttttcatgaa gatagataac
      541 gatctccgga aattcagcgc gaaccgcctg taataagggc atggtgatgg atgacgcagc
      601 ggttcctggt gcaaagccaa tcgagacttg ccccgataat gcctgaccaa cgttatgcac
      661 cgccagttgg gcctgttcac actgacgtaa aatggcccgc gcatgggtat agagaatttt
      721 tccggcgtct gttggcgtaa cgccccgctt tgtacggatc aaaagttgtt gatttaactc
      781 accttccagt gtggcaacct gctggctgag cgctggttgt gcgatatgca atacttcagc
      841 agcctgggtc aggctaccaa tatctacaat ttttacgaag tatttcaggc gtctgaagtt
      901 catgttgcct ccggttttta agaatcggcc caagtgccgc cattacttac aaccagattg
      961 caagatgctt gccagtttta ttttggcgtt gatgtacaag ctaaccaact gtcaaataag
     1021 agattatgat agattcgtca tttgctcctt taatcagctg tcgcgttccc ctgccctata
     1081 aaaggagggt atgcaccacg atggttcatt acccaataag attgaaagct caccactttg
     1141 ttgaaattga cagcaaacaa acaaaaaaat gcatttcacc ctttgacatc accatgcact
     1201 gccattaata tgcgccccgt tcacacgatt cctctgtagt tcagtcggta gaacggcgga
     1261 ctgttaatcc gtatgtcact ggttcgagtc cagtcagagg agccaaattc aaaaaagcct
     1321 gctttctagc aggctttttg ctttctaatt accaacgctc ttaaaacatc tgtcttgaac
     1381 cagaactaat ttgcacaggc attcccgatc gacgttgcaa cgcagcattt gcgcgattta
     1441 catcaacttc ttgcccgctg ataaacgccc gcaaagatgg ggttaccggc aatggcactt
     1501 ttcggtcaga ctcatattct gcacgattgc gcgacaacgg ctcatgaact tccagccagt
     1561 tcgagccatc tggttcagtg gtgtatttta ctggctggtc gataatttgc acacgcgtcc
     1621 caacaggaac attatcaaac agatatttga tatcgtcatt gcgcagacga atacagccct
     1681 gacttacccg gagcccaata ccaaaattgg cattggtacc atggatggca tacaacctgc
     1741 caatataaat cgcgtacagc cccatgggat tatcggggcc cgcaggaaca aatgcgggca
     1801 aactctcccc tcgtttcgca tattcgcgcc gagtgttcgg cgttggcgtc caggttggag
     1861 cttcttgttt acgttcaacg gtagtcaccc agttacgcgg ggtttctcgc ccagcctggc
     1921 cgataccaat aggaaagact tccacagtat tactgtctgg tgggtagtaa taaagacgca
     1981 tctcagcgac gttaaccaca atccctttac gaacagtggc gggcaaaatc agttgctgcg
     2041 gaatggtgag ttgcgagcca gacttcggca aaaaaacatc agcgcccggg ttcgcttcca
     2101 gcatgttact taacccttgc ccgtattgtg cggcaaaagt ctccagcggc tgggtattgt
     2161 gatcaggaac agttacagta agcgactgcc ccactaaacg gctaccctct ggaggtaatg
     2221 gataagttac cgccaggcta ttatggctgg caaaaagcag agcaaatgag caaaaaatat
     2281 ttacacgacg catcatgtcc ctttcctaag tcgcgaaagc tatccgttaa gtatagcttt
     2341 tatcagactt ttcgttttta actgttcaaa tcagaagtcg tattccccgg tagaacaata
     2401 ttactggcag caagttcgcc catgttgtta tatatcgcac aggcagcttc gatgatgggc
     2461 atcgccagag ctgcgccact accctcacct aaacgcatct ccatattgag ataaggctcc
     2521 agccccaaat gcgacagcgc aatacgcgcc cctttttctg ccgacaggtg agaaggaatg
     2581 agatacggtt tgattgcagg agacatctgg caggctgcga gcgcggcagc ataagaaaga
     2641 aatccatcca gcaacacggg taaaccgcag gaagcagcgc ctaacatcac gccagccatt
     2701 ccgaccaaat caaatccacc cacttttgcc aggacatcaa cgccatcctg cggatttggt
     2761 tgattcaacg taatcgcccg gcgcacaaca tcaattttat tagccagttt atctgtcggc
     2821 aggtttgcgc caatcccaac cacttcttca ggagcccggc cagtgattgt gctgactatt
     2881 gccgctgccg gtgtcgtgtt tgccatcccc aggtcaccta caccaaacag cgtaacaccg
     2941 ttttttgcca gctcccgcgt ataacatatg acgtccaaaa gcaacttttc tgccagatgg
     3001 cgactcattg ccggagctga agcaatattg ccactacctc gagcaacacg catgttgata
     3061 agcccgggga taggctcagc agtatcaata ccaacatcaa ttacgtagac gttagcgccc
     3121 gcttgtgctg ccagcacaca cacgccggtt gttccgcggg tcatattttc agcctgaatg
     3181 gctgtcactt cttttgggga aatagcgacc ccttcctccc agacgccgtg atcggcacac
     3241 atgaccagta ccgctttttt gcccacatgc ggaacgccat tcaaccccgg cattcctgcc
     3301 agttgtatgg caagcgcctc cagccttccc aggctaccaa caggtttgag taacccgtcg
     3361 atatgccgtt gtgcacgcga catagcggca ggatcaatag cagggatcgt attcagtaaa
     3421 tcggcaagtg tttgcatgtc acgtctcttt atagcagtgc cagcaggaag accagttcac
     3481 caagctctat agcagctccc agcgtatcgc cggtttgccc gcccagcgta cgtttgagaa
     3541 gctgaccgag gatgaaaatc gccaccatcg tcaccaccat agcagccaca ccatgcatac
     3601 ccggcaataa tacggcagca aaaatagcgg ctaagccgag tgtgacgcag gtttgtcgcc
     3661 catcaatttt gccaatgaac acattgccaa gtccttcttc ccgggcataa cgatgacgat
     3721 acatcaataa tgcggcagtc ccgcggctga ccgcacatgc tgccgccagc gatgcaagga
     3781 tcggttcgcc acgcagagcc aactcgctta ataccagaat ctttgccagt acaacaaaaa
     3841 tcaatgccag accgccgtgg gtgcctaaac ggctatcacg catgatttcc aacatgcgat
     3901 cgcggctacg tgcagaaaat acgccatcac aggtatcggc gaggccgtcc aggtggaatc
     3961 ccccggtcat cagcgccagc acaagtacgc taaacagtgc cgccagtggt acgccacacc
     4021 atgcctgcag caccatgaag accagcccgc taatcgcgcc aagcaataat ccaattaaag
     4081 gaaaagtaat aataccgcga gaataatgct cgaaatccag tccctgggac cagcgacgcg
     4141 gtacaggcag acgcgtaata aaagagagca tcgcccaaaa taatttactc atttgatttt
     4201 tactccaata cccgaaacca ccaaccatac ttcatttgcc gcagcggcca gctgctgatt
     4261 tacccgcccg gcaatatcac gaaaatgtcg tgccagacga ctctccggca caatccccat
     4321 tcccacttcg ttagtcacta atacaacctt tgcgggacaa cgttggcagg cagcaatcaa
     4381 cgactgaatc tcagcattaa tcgcctgttc catcgcctga taatcccatt catcagggtc
     4441 tttatcgccg ccataatcaa acaacagatt agtcaccatg gtggtaacgc attccagcaa
     4501 tacagcctca tgcgggttaa tgtctgcatg aattaattca tcaagatgtt gccagcgctc
     4561 cactgtgtgc cagtgcgccg ggcggccttg ccgatgatgt tctatccgtg cagccatctc
     4621 atcatcaagg atttgtgagg tggcgatata cagaacctgc gaagagtccc caataagcgc
     4681 ctctgcatgg cgactcttcc cgctccgtgc gccccccgtc accaaaatca tcataccgac
     4741 tcctgattca attgtatgat ggtattcatt gaacagaaca atggatcccc caccgttgct
     4801 gaccgcaaaa catttacgcg attaatactg cgcgtaatat aattaaaaat ccagcattct
     4861 caatccatta cgaaagaaga tggcattgcg gaatagccag acttcctcgt gttaacgtcc
     4921 gacaaaatgc gtcattgaca gcggtttctc tggaatgaca gatttcattc agataatgga
     4981 ttaatgctac agccccacca gccggaccag tcagcaaacg ggcattgtgt tctgcaatca
     5041 aaattaccgt cgttccctca gaatgagcga gccattctgg tacggatatc tcgctttcag
     5101 ctgctatcaa tagcagcact gaataatcgc tatacgcatc tgataacgat tgcacaggcc
     5161 caccatcaag aggctggaaa tcttgctgcc agtcctcacc aacaaaacag gttattggca
     5221 atatttcagc cagatgtttc gccagatcca catctccaga taagaggcaa aaaagagcta
     5281 tcgattcggg ttttatcgtt ttcatgcctc tcgccctcca cttaatttcc tgtaatggcg
     5341 gtctggttga taagccatga aacaatattg ccacaccttg aatgcagccc tggtgcctgt
     5401 cggcactgcg atgaaagaac taaatgatgc cgccataccc tgatcctttc agggtggtag
     5461 gggtataaag acgcgccagt actctaccgg acgcttccgg gcaacccact tcccaccgaa
     5521 gttgttggta ttgactgaca gactggtctt ctggcttagc gtcatcctca cccgaccttc
     5581 ccaatctcgc gatcagtggc attttcgggt ttgtcagcat tacagcagcg ggggctgcga
     5641 gggattttca ccctcttccc aggcacaaat gtgccacacc tgtcagcgta tgttatgagc
     5701 tgatttcact tttcccataa cacgtaaata acgttaatta ttcattccag cgtttattta
     5761 aaccttccct ttttttagca tttattctat accgtttcca tcgccgcatt ttcactcact
     5821 tgctgtcgat acacctgcgg cgtaacctga taagtctgac ggaaaacttt gcaaaaataa
     5881 ctgtattgtc gtggggatta ggcgcacgag tctggcgcac cgaatctgac cgagatttgt
     5941 tctctttaat aaccacaaaa ttgagttgtt ttttgaggca attttccaca atcaacttca
     6001 acaaatcagc agaggccata actcttgagt aatccatctc aggaactttg cggaattcat
     6061 taagtaattc gggatctgcc tgccagcaat catcgacatt cagaatatcg accaaatcga
     6121 catcatcatt acgcagacga acttgcccgc acaatacaaa accgactaaa tgtccggcga
     6181 ttaccagtgg aatagagaaa tcggttaatc cggcatgaca gcgataaata caaggctgat
     6241 cggatttaga ggcttccagg cccccacaac gatcgctcat gcgacatcga gtgcttagct
     6301 gagggtgttg ccgcatcatc tgacaaaatg gagtgaagtt aaacagctcg gaaatttcat
     6361 cgccgtgaat attgaccacg acaaccgcaa ggctggtcgc ctgcgcaaaa tcctgcgcga
     6421 ttttattaat gagttctgaa ttaagtgtcg ccgcagaaat catgataaaa cctcccagtt
     6481 aatttattgt tatagcaaaa caattgtttt acatttaatc ttcacatcat ccctgatgat
     6541 ttactgacga gaagcacaaa ggacaataac aaaagcctcc gtgaagcggg agacttttta
     6601 ataagttcgg aaagcagatc acaaacagta aaaaaataaa tgtttataac tgccagagta
     6661 tttcgttatt cgctaattcc tttaggtgta acactattga caggtaaatt ctttgcaatg
     6721 agatagcgat aaatcgccgc accaacacat gccccaatga tcggtgcaat aatgggtaca
     6781 atgaaataag gaatattgcg tccaccagtc atcgcaattt ctccccaccc cgcaagccag
     6841 gtgaaaatct taggtccaaa atcacgcgct ggattcatgg caaaaccggt taatggtccg
     6901 gtagaagcac caataacagc aaccagaata ccaataagta aaggtgcaag cggtcctttg
     6961 ggtacgccat taccgtcatc ggtcaacgcc ataatcaacc ccattaacat ggacgttatg
     7021 accacttcga ccaacgcagc ttgccataca ttaatcgccg ctgccggata agtactgaag
     7081 atactggcga gttgtaaact ttctgcgcta ccacgcacca tattgtgagc agattcaaaa
     7141 tcggtaaaca gactactgta tagagaatat gccagaactg caccaccaaa ggcacccgct
     7201 aattgcgcca gacaaaatgg cagcacttta cgggctggga aagaggcaaa cagccacaga
     7261 gcaatggtaa ctgccgggtt gagatgagcg ccggaaatac ctgccgtaag gtaaacagca
     7321 agcgaaatcc ctaaccccca aatgatacaa atttcccaca gtcccagact tgccccggca
     7381 actttcaggg cgcacaaaca accaatgcca aaaaagagga acagtccggt gcccaaaaac
     7441 tcggcaacgc attgcgcctt gagtgaatca ttcatagtga cacctttttt acggagtttt
     7501 tctttatcag ccaactatgc gctgactgtt gtatataccg agcacaacta taaaaaattg
     7561 ctctgcagaa aatattgcca acatgcatga cagactcgtg taaaaatcgt gctaacataa
     7621 caaattattg tcattaccga atcatgcaac acagcgttag tcagtgttga aagtaataat
     7681 tcccaccaaa cttttacaat aaaaaattgc catcatgaca taataaaatt gaaacaaata
     7741 tcacagttcc ttcttcgaac taaactcaac caaatattac tcgcgattaa actcactagc
     7801 caacgcaatt aattcacatt gaaatatcca caaaacaacc aataaattaa caacatatag
     7861 ttcataaaag caattttata atatttgttc tctattaatt tctctcatta gccgattaag
     7921 tcagccattc tttttcctcg ctttttttta tagtcatatc gtcaaaaaaa tccaggcgag
     7981 gtctttatgc aacaagaagc gttaggaatg gtagaaacca aaggcttaac tgccgccata
     8041 gaggccgcag acgccatggt gaagtcggca aatgttttgc tggtcggcta cgaaaaaata
     8101 ggttccgggc tggtgaccgt catcgttcgt ggtgatgtcg gggcggtaaa agcggcaaca
     8161 gacgcgggtg ccgcagctgc gcataacgtc ggcgaagtaa aagccgtaca cgttattccc
     8221 cgcccacata ccgatgtcga aaaaatctta ccaaagggaa ttaacccatg agcagcaatg
     8281 aactggtcga acagatcatg gcgcaggtca tcgctcgcgt agcgacgcca gagcatgagg
     8341 ttctcccgaa aaccactcat caaatacgag agacggctat ggcagaaaaa agctgcacgc
     8401 taacagaatt tgttggcacc gctattggcg acactgtagg tctggtcatt gcgaacgttg
     8461 atagcgccct gttggacgcg atgaaacttg aaaagcgcta tcgttccatt ggcattctgg
     8521 gtgcgcgtat tggcgcaggg ccacacatta tggccgctga tgaagcggta aaagccacca
     8581 acactgaagt tattagcatt gaacttcctc gcgatactaa aggcggtgcc ggacacggct
     8641 cactgatcct cttaggtggc aacgatgttt ccgacgtgaa acgcggtatt gaagtcgccc
     8701 tgaaagaact cgatcggacc tttggcgatg tctatgccaa cgaagccgga catcttgaac
     8761 tgcaatatac cgcccgcgcc agctatgcac tggaaaaagc gtttggtgca ccactgggac
     8821 gggcttgcgg cgttattgtc ggcgctccgg ctgctgtggg tgttctgatg gccgacaccg
     8881 cgctgaagtc cgccaatgtg gatgttgtgg catacagctc cccagctcaa gacaccagct
     8941 acagcaatga agtcattctg attatctcgg gtgactccgg cgcagtccgc caggcggtca
     9001 tctccgcacg cgaaatcggt aaaaccgtgc tttcgactct cggcgataca ccgaaaaacg
     9061 atcacccttc ctacatctga attctctgag gcagatttat gagatcgaaa agatttgaag
     9121 cactggcaaa acgcccggtc aaccaggacg gtttcgttaa agagtggatt gaagaaggct
     9181 ttattgcgat ggaaagcccg aacgatccta aaccgtcaat caaaattgtt aacggcgtcg
     9241 ttaccgaact ggatggaaaa cccgccagcc agtttgacct gatcgaccac tttatcgccc
     9301 gctacggcat caatctcgcg cgcgccgaag aagtcatcgc gatggattcg gtaaaacttg
     9361 ccaatatgtt gtgcgatcca aatgtcaaac gtagcgatat cgttcccctt accacagcaa
     9421 tgacgcctgc aaaaatcgtc gaagtggttt cgcagatgaa cgtggtagag atgatgatgg
     9481 cgatgcagaa aatgcgcgcc cgtcgaacac catcacaaca ggctcacgtc accaacgtca
     9541 aagataaccc agtacaaatt gccgccgacg cagctgaagg cgcatggcgc gggtttgatg
     9601 aacaggaaac caccgttgcc gttgctcgct atgcaccgtt caacgccatc gccctgctgg
     9661 tcggctcaca ggtgggacgt ccgggcgtct tgacccagtg ttcactggaa gaagccaccg
     9721 aactgaaact cggtatgtta ggccacacct gttacgccga aacaatttct gtctatggta
     9781 cagaacctgt tttcaccaat ggtgatgata ccccgtggtc aaaagggttt ctcgcgtcat
     9841 cctatgcttc tcgtggctta aaaatgcgct ttacttccgg ctccggctct gaagtacaga
     9901 tgggttacgc ggaaggcaaa tcaatgcttt acctcgaggc tcgttgcatt tacatcacca
     9961 aagccgcagg cgttcagggt ctgcagaatg gttcggtaag ctgcatcggt gttccatcag
    10021 ctgtgccatc aggcatccgc gcagtattag cggaaaactt aatttgctca tcgctggatc
    10081 tggaatgcgc ctccagtaat gaccagacct ttacccactc ggatatgcgc cgtaccgcac
    10141 gtttcctgat gcaatttctg ccaggcaccg actttatctc ttccggtttt tccgctgtgc
    10201 cgaactacga caacatgttt gctggttcaa acgaagatgc tgaagacttc gatgattaca
    10261 acgtcattca gcgtgacctg aaggttgatg gtgggctgcg accagtacgc gaagaagatg
    10321 ttatcgctat ccgcaataaa gccgctcgcg ctttacaggc tgtctttgcc ggtatgggac
    10381 taccgcctat taccgatgaa gaagtcgaag cggcaaccta tgcccacggt tcaaaagata
    10441 tgcctgagcg caatattgtg gaagacatca agttcgccca ggagattatc aataaaaacc
    10501 ggaacagcct ggaagtggtg aaagccttgg cgcaaggcag ttttaccgat gtcgcccagg
    10561 acatgctcaa catgcaaaaa gcaaaactga ccggcgatta tctccatacc tcagccatca
    10621 tcgtcgatga cggacaagtg ctctctgcgg tcaatgacgt caatgattac gccggaccgg
    10681 ctacaggtta ccgcctgcaa ggggaacgct gggaagagat caaaaatatt cccggtgcac
    10741 ttgatcccaa cgaaattgac taagggtgag aaatggaaat taacgaaaaa ttactgcgtc
    10801 agattatcga agacgtgctg gcagaaatgc aacccagcga caagtccgtt tcatttcgcg
    10861 cacctgtctc tgcaactgtc ccttccgcgc cagataccgg aaattttctg accgaaattg
    10921 gcgaagccca acaaggcact cagcaagatg aagtcattat tgctgtaggc ccggcgttcg
    10981 gcctggcgca aaccgtcaat atcatcggta ttccgcataa aaacatcttg cgcgaagtga
    11041 ttgccggtat cgaagaagaa ggcattaaag cccgtgtaat tcgctgcttt aaatcgtccg
    11101 atgtcgcatt cgtagcagtc gaaggcgatc gtcttagtgg ttcaggtatt gctatcggta
    11161 ttcagtcgaa aggcacaacg gttatccacc agcagggact tcctcccttg tctaacctgg
    11221 aacttttccc acaagcgcct ttgttgacgc tggatactta tcgtcagatt ggcaaaaacg
    11281 ccgcgcgtta tgcgaagcgc gagtctccac aaccggtgcc gaccctcaac gatcaaatgg
    11341 cgcggccgaa ataccaggcc aaatctgcca ttttgcatat taaagagacg aagtacgtgg
    11401 tcactggcaa aaaaccgcaa gaactgcgcg tgacgtttta acaaaggaaa ccttcatgaa
    11461 taccgatgca attgagtcga tggtcaggga cgtgttgaac cgcatgaaca gtttacagga
    11521 tgcagctcct gtttctgcgg tccccaacgc atcaatactg agcgccaaag tgacggatta
    11581 cccgctggca aataaacatc cggaatgggt taaaacagcg accaataaaa cgctcgacga
    11641 tttcactctg gaaaatgtgc taagcgataa cgtcaccgcc ctggatatgc gcattacccc
    11701 tgaaacattg cgtattcagg ctgcgattgc cagagatgct ggtcgcgatc gtctggcgat
    11761 gaattttgaa cgcgccgctg agcttacctc agtaccagat gatcgcatcc tcgaaatcta
    11821 caatgcgtta cgcccttatc gttcaactaa gcaagaactt attgccattg ctgacgatct
    11881 tgaacagcgt tatcaggcca aaatttgtgc tgcatttgtc cgtgaagctg cggaacttta
    11941 tgtcgagcgc aaaaaactga aaggcgacga ttaaggacga actatgcgat atatagcagg
    12001 cattgatatc ggcaactcat caacagaggt tgcgctggca acgctcagtg caactggaga
    12061 gttgtcgttc gtgagtagcg ccctggagga aaccaccgga atcaaaggta cgttgcgtaa
    12121 cgtccatggt attcaggagg cgctcgcaca ggcaacgaaa aaagtcggca tcaatgttag
    12181 cgatatttcg cttattcgta tcaacgaagc aactccggtc attggtgatg tggcgatgga
    12241 aaccatcact gaaactatca tcaccgaatc aaccatgatc ggtcacaacc cgaaaactcc
    12301 gggcggagtg gggctgggcg tagggttcac catcactccg caagagttat taacacgccc
    12361 ggcggacaca ccttacatcc tggtggtgtc atcggcattt gattttgccg atatcgcgac
    12421 gatgataaac gcctccgtac gcgccggata tcagctgact ggcgttattc tgcaacgcga
    12481 cgatggcgta ctggtcaata accgcctgga aattccttta cctattgtgg atgaagtcct
    12541 ctacatcgat cgtattccgc tcggcatgct ggcagcaatt gaagttgccg ttcccgggaa
    12601 agttatcgaa accctgtcta acccgtatgg gattgccacc gtattcgccc tcaatgctga
    12661 agagactaaa aatattgttc ctgtcgcgcg ggcgctaatc ggcaaccgtt cagcagtggt
    12721 agtgaaaacg ccctctggcg acgttaaagc ccgttcgata cccgccggaa atatcgaact
    12781 gctttctgcc gggcgcacaa cgcgcgttga tgtagctgct ggtgccgatg ccattatgaa
    12841 agcggtcggt gagtgcccaa aactggaaaa cgtgacggga gaaccaggca ccaacatcgg
    12901 cggaatgctg gaacatgttc gccagaccat ggcggaactg accaacaaac ccagcaacga
    12961 aatttttatt caggatttgc tggcgataga tacctctgtc cccgtcagcg taaccggtgg
    13021 tctggcaggt gaattttcac tggaacaggc tgtgggtatt gcctctatgg tgaaatccga
    13081 ccgtctgcaa atggcgatga tcgccagtga aattaagcaa aaactgcatg ttgatgttca
    13141 ggtcggaggc gcagaagcag aagccgccat tcagggggcc ttgactaccc ctggaaccac
    13201 acgtccactg gcaattctgg acttaggtgc aggctctacc gatgcctcca tcatcaatca
    13261 gagtggggaa atcgttgcca ctcaccttgc gggagcgggc gacatggtaa cgatgattat
    13321 cgctcgtgag ctgggcttaa atgatcgtta tctggcagaa gaaatcaaaa agtacccgtt
    13381 agcaaaagta gaaagcctgt tccacttacg ccatgaagac ggtagcgttc agttcttccc
    13441 tactccccta tccccccatg tatttgcccg cgtctgtgta gtgaaacccg acgaactggt
    13501 gcctattccc ggtgatttga cgctggaaaa agtacgcgct gttcgtcgca gtgcgaaaga
    13561 gcgtgtcttc gtaaccaatg ccctgcgcgc gctacgtcag gtaagccctg ccggaaatat
    13621 tcgcgacatt ccgttcgtcg ttctggtggg tggttcttcg ctggatttcg aagtaccgca
    13681 attggtcacc gacgcgctgg cacattatcg actggtcgcc gggcggggga atatccgtgg
    13741 tagcgaaggt ccgcgcaacg cggtagctac tgggctactc atcgcctggc acaaggagtc
    13801 gattcatgga aagtaaagag cgtgcaccag ccatcgttgt catggagatc ggtgactgta
    13861 tcactacatg ggatgaggtg cttcttggca ttgaagagga aggtattccc tttcgcattc
    13921 aacacattcc gtcaggtgaa gttatcgata gcgcctggct ggctgctcgt caatctcccc
    13981 tgttggttgg cattgcctgc gaccgggaag aactgattgt gcattacaaa aacttaccgg
    14041 catcagcgcc actgtttacg ctgacgtatc aacaagataa ccatacccgg cgcagcatcg
    14101 gcactaacgc tgcccgcctg gtaaaaggaa ttcccttccg ggaattccac tcataatcca
    14161 caggagaaac gctgtatgaa caacgcacta ggactggttg aaacaaaagg gttagttggc
    14221 gcaattgaag ctgcagatgc catggttaaa tccgccaacg tgcagctggt tggctatgaa
    14281 aagattggtt cgggtctgat tacggtgatg gtccgcggtg atgttggcgc agtaaaggcc
    14341 gccgttgatg cggggagcgc tgcggcaagc gccgttggcg aagtgaaatc ctgccatgtc
    14401 atcccgcgcc cacacagtga tgttgaggct attttaccta aatcggcata accccatttg
    14461 ctaaggagca cagcgtgaag caatcactgg gattacttga agccagtggt ctggctctcg
    14521 ccatcagttg cgcagacgct atggcaaaag ccgcgtccat cacgctggtt ggccttgaga
    14581 agaccaatgg ctcaggctgg acgctcatca aaatcaccgg tgacgtcgcc tcggttaatg
    14641 cggcgatcgc gacgggagcc agtttcgccg gacagcgaaa tggccttgtc gcacacaaag
    14701 tgattgcccg cccgggagac ggcattctgc cccacacaac tgaagcattg accgtgacgg
    14761 ttgaaacaag caaaacacca gcaacaaccg tcacctcaga gattatcagt tgtaatttgt
    14821 gtcttgaccc ggcatgtcag cggcaaaaag gtgagccccg ctcactttgc ctgtacgcca
    14881 ataagcgagg ggagtagcag tggataaaca aaccttgcag gaaacagtga gccagattct
    14941 ggatgagatg cgcgagcgcc ctatcccact gggcgtttcc agtcgccata tacatctttc
    15001 atcagcggat tatttgcgtc tttttcctga acaaccgatt agcgagaaaa agccgttaat
    15061 gcaacctggc caatacgctg ctgaacaggt tgttacgctg gagggaccaa aaggcaaatt
    15121 gcacaacgtt cgcttgttgg ggccgctacg caatgcaagc caggtggaaa tttcccggac
    15181 ggacgccaga acgttaggga tcaccgcccc gctacgcatg tcaggcgatc tcagcggcac
    15241 tccggggatt cgtttgataa gcccatttgc ggaactggag atcacatctg gcgtcattgt
    15301 cgcgcaacgg catattcata tgtctccgct ggatgccctg atttatggcg ttacgcatgg
    15361 cgataaagtg gcggttgcca ttgaaggtga tgagcgacga ctgatattcg aaaacgtcgc
    15421 tgttcgcgtc tctccggata tgcgtcttga aatgcatatt gataccgatg aggccaacgc
    15481 cgcaggcgct gatagccctg acgtttttgc cacactggtg tcagtgccat gaataacatg
    15541 ctgatgcaac gtattgttga cgaagtggtt tttcgcctta aacaacgtgc gggaaaaaca
    15601 ctcgtgctga ctgtttttca attgcgagac gccagcgttc aagagagcgt tcatcagtat
    15661 gcgtcattac aaatccgata tgtcgatctg ccgctgctgc gacaacttgc ggagaacgaa
    15721 acgtcagatc gagcatctat ccagatacac gaagcgttag cctggggaat gcacattcaa
    15781 ttgtcgttgc agcgtcattt cctgaacgcc atcgaattga agactctcgc ccgtctaccg
    15841 ctaagctggt gcgacgaaca agggcagccg atctaccttc atcataatcg acttttaagt
    15901 tatgccgata tcgcacagtt aagctcggga atactggtgt tacagcggaa atgttgtgtg
    15961 accgcactgg ctcgtgaagc ggcaattacc cgaaatattc agttaatcag gcaggagtaa
    16021 cctatgcatc tggctcgagt cactggcgcg gtagtatcca cgcaaaaatc accttctctg
    16081 atcgggaaaa aactgctgct ggttcgccgg gtcagtgctg acggcgaact gcctctcacg
    16141 ccattaaccg gcgacgaagt tgccgttgat tcagtcggcg caggcgttgg tgagttggta
    16201 ctcctcagca gtggttccag cgccagacat gtgttttcag gccctaacga ggccatcgac
    16261 ctggcagtgg tcggcattgt cgatacgctt tcgcgctaag gagctaaaag atggcaattt
    16321 atacccgaac aggtgattca ggaagtacgt ctttatttac cggacagcgc gtcagcaaaa
    16381 ctcatctacg tgtcgaaacc tatggcacgc tggatgaact gaacgcgaca ctcagtctgt
    16441 gttattgcgc caccgccatc gaaagccatc gcatcttact ggaagccatt caacaacaga
    16501 tattttggtt tagcgccgaa ctcgccagtg aaagcgaaca gccgtcagcg caacagcgtt
    16561 acatcggtac ggaagaaatt gccgcgttgg aaaacgcgat cgatagtgca atgaacgccg
    16621 tccccccagt tcacagcttc attctgcctg gtcgatgtga agccgcaagc cgcatgcatt
    16681 tcgcccgcac ggtggctcga cgggctgaaa gacggctggt tgaactgaca acggaaacca
    16741 ccgtccggaa tgttttgctg cactatatca accgactttc cgattgcctg tatgccctcg
    16801 cccgcgtgga agacaacgtt gctcatcaaa acctgatgat tcaggaaatc acaaaacgtt
    16861 atcacgaagc caaccacaca ccagcattga aggaacgcac aatgccgctc acttttcagg
    16921 atcttcacca gctcattcgt agtgcggcaa tgcgcgcgga tgaactgcat attcccgttg
    16981 tcatcagcat tgttgatgcc aacggaacag aaagtgttac ctggcggatg cctgatgccc
    17041 tgttggtcag cagtgaactg gcaccgaaaa aagcctggac cgccgtggcg atgaaaacgg
    17101 caacccacaa gcttgctgat accgtgcaac ccgacgcgcc gctttacggg ctcgaaagcc
    17161 atatgcaagg aaaagtcgtc acttttggtg gcggcttccc cctctggcgt gacggaaaat
    17221 tgcttggcgg gcttggcatc agcggcggta gcgttgaaca agacatggat attgcccaaa
    17281 gcgcaatggc ggcaattaac gtgggagtaa accaatgaat aactctgaat tagaaacact
    17341 gatccgcaat attctgcgtg agcaacttgt ccccgctaca ccacgaacgc aaagaaacgc
    17401 tattttccag actgtcgatg aagcggtctg cgcagcacac taggcctttt tacgctttca
    17461 acagtgcccg cttaaaaccc gtagcgccat tatcagcgcc attcgcgaag aattaatgcc
    17521 ccgccttacc gaactggcag aagaaagcgc caaagaaacg gggatgggaa ataaagaaga
    17581 taagtaccta aaaaacaaag cggcactcga taacacgcca ggaattgaag atttaaccac
    17641 cacagcacta accggtgatg gtggcatggt tttgtttgag tactctccgt ttggggtgat
    17701 tggctcggtc acgccaagca ccaaccccac ggaaaccatc attaacaaca gcatcagcat
    17761 gctggcagca ggtaatagcg tctacttcag tccacacccg ggtgccaaaa acgtttcgtt
    17821 aaaactgatt accacaatcg aagacatcgc gtttcgctgt agtggcattc ataacttgat
    17881 cgttaccgta tctgaaccga cctttgctgc caccgaacag atgatggcgc accctaaaat
    17941 cagcgttctg gcgattaccg gcggtccggg gatcgtcgcc atgggaatga aaagtggcaa
    18001 gaaagtgatt ggtgccggag cgggtaaccc tccgtgcatc gtcgatgaaa cggcggatat
    18061 tgtgaaagcc gccgaggaca tcatcaacgg cgcttcattt gattacaacc tgccgtgtat
    18121 tgctgaaaaa agcctgattg tcgtggcatc agtggcagat tatttaattc agcagatgca
    18181 aagttttggc gcacttcttc ttaactatga gcaaactgag aaattgcgtg caatttgcct
    18241 gccagacggt agcgccaaca aaaaactggt tgggaaaagc ccatcagcct tgctggaagc
    18301 ggcaggcctc cctttgcccg caaaagcgcc gcgcctactg attgctgttg tcgacgccga
    18361 tgatagttgg gttacttgtg agcaactaat gccaatgctg cccattgtga aggtcaagga
    18421 ttttgacagc gctctaaccc tggcgctgaa agtggaagac ggcttgcacc acacggcaat
    18481 tatgcactct cagaacgtct cacgactcaa ccttgccgcg cgcgttatgc aaacctcaat
    18541 atttgtcaaa aacggcccct cctgggcggg tattggcgtc ggcggcgaag gctttacgac
    18601 ctttaccatt gcaacaccga ctggagaggg tacaacgtca gcacggactt ttgcccgctc
    18661 acgacgctgc gtgttaacca gcggcttttc aattcgctag agaaacggaa atgaatactt
    18721 tttcactgca gacgcgttta tacagcggac caggaagtct caaggtactt agccgtttct
    18781 ctcatagaca catctggatt atttgcgatg cctttctggc acgctcacca cttattgaca
    18841 cgttgcatca ggcgctgccc gacagtaatc gtctcagtat ttttagcgaa ataacgcccg
    18901 ccccaactat ccagaccgtg gtgaaaggca ttgcgcaaat gcagacttta cgcccggatg
    18961 tagttatcgg ttttggcggc ggttctgccc tggacgccgc aaaagcaatt gtctggttcg
    19021 gtcgccagtg tggcattgaa attgaaacct gcgtagccat tcctaccacc agcggtatcg
    19081 gttccgaagt gaccagtgct tgtgtcatca gcgatcctga gaaagggatt aaatatcccc
    19141 ttttcgataa tgcactttat ccggatattg cgatccttga tccatctttg gtcgtcagcg
    19201 tgccaccggc gataacggcc aataccggga tggatgtttt aacccatgcg cttgaagctt
    19261 atgtttctcc tcgcgccagc gacttcacgg atgcactggt cgaaaaagcc gtgcaaattg
    19321 tttttcaata cctgcccact gcagtgaaaa aaggcgattg tcttgccacc cgtggcaaaa
    19381 tgcataatgc atcgacgctt gcaggaatgg cctttagcca ggccggactc ggtttaaacc
    19441 atgcaattgc tcatcaactc ggcggacagt ttcatctgcc gcatggactg gcgaatgcgc
    19501 tcctgctcac ggcagttatc cgctttaatg ctggcgatcc gcgcacagcc aaacgctatg
    19561 cccggctggc gaaaacgtgc catttatgcc cggacaacgc caatgacacc gccagcctga
    19621 atgcacttat tcaacatatt gagcagctta atacaacctg tacgctgccg actcttgcca
    19681 atgcgctgaa agaaaaaaaa gcagaatggt caatacgcat accagatatg gttcaggcgg
    19741 cgctggcgga tgcaacgttg cgtaccaacc cgcgtgcagc tgatgcctcc gcgattgcag
    19801 aactgctcaa ggggttgtta tgaacgtcgg attaatctcc tgcgacgacc agaccatccg
    19861 cgagcgagta caagcagcag gtgtcgtggg tgctggcggc gcaggttttc caacccatat
    19921 taagctacaa gcgcgtgctg acactttcct ggtgaatgcg gcggaatgcg aacccatgtt
    19981 gaaagtagat caacagttaa tggcgcagca agccagccgc ctgcttcgcg gtgttcacta
    20041 tgcgatgaaa gccaccgggg ccagtagcgg aatcatcgcg ctgaaagaaa aatatcaacg
    20101 ggcaataaac gcactcacac cactgttacc accggatatc cgcctgcata ttttgcccga
    20161 cgtttacccc gcaggagacg aagtgctgac catctggatg gcaactggtc gccgcgttcc
    20221 acccgctgca ttacccgtta gcgtcggtgt ggtggtcaat aatgttcaaa ccgtacttaa
    20281 tattgccaga gccgtcgagc aacagtatcc agtgacgcgc cgcacactca ccgttaatgg
    20341 tgcagtggcg aagccaataa cggtaaccgt ccccatcggc atgtctttac gtgaagtcct
    20401 ggcgctggcg ggcggcgcaa cagtggatgc tcctggtttt atcaatggcg ggccgatgat
    20461 gggaagtttg atcacatcgc tcgacacacc agtcagcaaa accactggcg gcttgctggt
    20521 tttacccaac tctcattcac tgatccaacg tcgaatgcag aatgaccgca gcgtacttgc
    20581 cgtcgcgaaa actgtttgtg agcaatgccg cttgtgtacc gatctctgcc cacgtcattt
    20641 aatcggtcac gaactggcac cccatttatt agtacgtgcg cggttaacta ccaacagctc
    20701 gccacgccgc aactgttact gacagcgctc acctgttcag agtgcaacgt ctgcgccagt
    20761 gtcgcctgtc cggtaggaat ttcaccaatg cgcataaacc gcctgcttaa gcaggaatta
    20821 cgcgcacaaa atctccgcta cgaaggtgcc ttaaatcctg cagatcccat ggcaaattat
    20881 cgactgattc ccgtaaaacg gctggtaacc agacttggct taaccccgtg gtatcaggat
    20941 gcgccattgt ctgaacaggt gcctcagccc gaaaaagtga cgttattgtt acgccagcac
    21001 atcggtgcca gcgcaattgc gtgtgtacaa aaaggcgatc gcgtcgttca tgggcaatgt
    21061 gtaggtcaaa ttccacacgg cacattaggt gcccccattc atgccagtat tgatggcatg
    21121 gtcagcgatg tgacagaaaa cgcgattacg cttgtgagag gttaaccatg tcgaaagcgg
    21181 taggtatttt agaacttagc agtatcgcga aagggatgga aaccggcgac gccatgctga
    21241 aaagcgccaa cgttgagtta ctggtcagca aaacgctatg ccccggaaaa tttctgctga
    21301 tgctcggtgg cgatgtgggc gcggtgcaac aggccatcaa cagcggagcg tcccttgctg
    21361 gtgaaatgct ggtcgacagt cttgtcctgc cgaatattca tcccagcctc ctttctgcaa
    21421 tcagcggact taaccatgtt gaacagcacc gggctgtcgg cgtagtcgaa acctggagtg
    21481 tcgccgcctg cattgacgct gccgattgtg ctgtcaaagc tgcaaatgtc attcttgttc
    21541 gcgtacatat ggcttttggc attggcggta aatgctacat ggtggttgct ggcgatattt
    21601 cagatgttaa taacgcggtc tctgccgcca gcgtgcgcgc gggggaaaaa ggtttgttgg
    21661 tctatcggtc ggtgattgcc cgtccacatg aagccatgtg gcgtcagatg gtggagggat
    21721 aatgacaact cagcacacaa ctgaacggat gattcaggaa tatgtcccag gtaagcaggt
    21781 cacgctggca catgtcatcg ccaatccagg gaaggatctg tttaagaaat tgggtttacc
    21841 agacaccatt tccgctattg gtattctgac aattaccccc agcgaagcat ctatcatcgc
    21901 atgcgatatc gccacaaaat caggcgctgt agagatcggt tttcttgatc gttttaccgg
    21961 tgccgtagtg ctgactggtg atgtttctgc gatcgaatat gcacttagcc aggttacccg
    22021 tacgctgggt gaaatgatgc gctttaccgc ctgccccatt acgcggacct gacgttatgc
    22081 aacgtatgat gctcatcggc cccagccagt gcggaaaaac gtcactcacg caatgtctgc
    22141 gtggcgaaaa tattcattat cagaaaacgc aggcaatcgt ctggtccccg accaccattg
    22201 atacaccagg cgaatacctc gaaaaccgca gcctctacag tgcactgttg gtcagtgcct
    22261 gcgaagcgga cattatcgcg ctggtgctta atgcggacgc accgtggtca cctttttcgc
    22321 caggctttac ctcgtcaatg aatcgcccgg tcattggagt agtaaccaaa gcagatttag
    22381 ccaacatgga acaaatatct ttggtgaaat gttggttacg ggaagctgga gcgcacaacg
    22441 tgttagtaac cagtgcggtt aataaccatg gagttgcaga acttttagcc ttgctgcata
    22501 cagaagaagg ctgtcgctaa tttcgacagg ttatcagatg ggaatgatga cggtgataca
    22561 cagaaaagaa tcaatgagca atgagggttg ccgggcaacc ctcattgaat aaaacaggaa
    22621 ttacttcgct tcgccgtttt tcatttcgcc catagctttc agctttttgg agatatcgcg
    22681 gcgttctttg gagagctcgg catttttgat gatgtaatcg tcaacgcgat cttcatagtc
    22741 acctttcatg ctggcgatga tgccctggat tgcttcaacg ctcatcccag gcttgatgta
    22801 atcgctcagg ttgtccagca gcaggacgcg cttctggttg tcacggatct ttttctcaac
    22861 gtcctgaatt tcacgttgca gtttgttctt acgacggaac agacgaacaa attccagtac
    22921 gtcctggaat gaaggcttgg tagtttccat ttttataccc ctgataatgt gagagtcgga
    22981 ttcgtttaag caaccgctat tcgttagggc caacattact catagctgcc gcagatgaca
    23041 atgcttttat cccttttcac tatcataccc tttatccttg ctgaatcgaa gcagcagcaa
    23101 gatgattctg aagttcagga attatttgcg caaggcccgg caaatcagat tcgatagcaa
    23161 ctcaagctga tgcgccgttt ccatgttcag ccacacataa ccatagattg gtgtttccac
    23221 taccttcagg tcatggtggt tattgagcaa ctgacgcagc tcttccacag catcgttcaa
    23281 tttttccgta ttggcaaaaa ccggctgcgg attaccttcg tacagcgcat gaacaaagct
    23341 cagcagtatt tgctgcatca tgtgctgggt atcacgaagt ttttgcgcgt tcaataacac
    23401 gaaatggctg gggcgcgtgg cccagtacgc attgatttgc aactccagca tacaaaccag
    23461 attgcggtta atggtctgga taccttcata tatcgatttt ggaatacggg tttctttgct
    23521 ggcgggcgca atcagcccac gcattttcac ggcatcggtc aggagttttt gtagatggct
    23581 ttccagacgt gggcgttcga gtaagttcgg tgagaatgca gattgataga cccgattata
    23641 ctcggtcaga cttttcgcca gttgaatgcg ccagtggatg aacgcccgtt gtggccagat
    23701 accggtaaac aacattgcca gtaaagagcc gaggatcaca tcgccgcttc gccataacgc
    23761 cgtatcaatt tcacctgtcg gggaaccaac aacaattgcc agcgtcaccc caatcaataa
    23821 tccttgatac ggtttcttgc ccagcgccag ccaaccgcaa agaaacatcg ccgccgcgca
    23881 ccagactaac atcagcggta acgaaattaa ctccagttgc agagcgataa gacctaaaat
    23941 cgaacccaac accgtaccgc caatacgttc gaaggcacga gggacaacgt taccccagaa
    24001 cgagattggc cccataatca ccaccatggt gaccagcggc caggtgcttt ccgggatagt
    24061 aaacaggcgg ataatgagaa aagtgagcag gaatgccagc gcgacccgag taccatgcac
    24121 tatgcggtaa tggcggtata cccggatttc aaacgggctt aatgacttat cggcacgcac
    24181 gcagaaactc cgtaatgaag attaaaagcg ctcataactc atctgtcggc cagaaggcgc
    24241 gcctctgaat tatccgacgg acaaaccggg aacagtgtaa tttcccggca ggagcgaaaa
    24301 aattgaaagg cgcatcttac tcttttcgct ttcaaaaaaa gagtggtcat cgcgttaaca
    24361 caccgccctg agatgaatta gtgatgtttt ggctgcaccg caacatacat ttcgatatcc
    24421 cagtacccat cttccgcgcc attgttcaaa taaacctcga agcagggctt tggtaacatt
    24481 tcataagcac tgtcctgcaa gaggctatta aagaactgat accagggttt agcaaaatca
    24541 tcaccgacta cacgagccac cgccaccgca tactgaccac ctgaaatttc tgtcagaatg
    24601 acgccctcac tgttttcggg aagcgtaaag ttattcggta ccgtcacaac ggtgtcgcag
    24661 cgtaattttt cggcgggtgt ttcatctgga ttgtcgtaat agacagcaac ccactccttc
    24721 ggcacaatat ttttgttatc tacccacatc atcaactgct caaagccttt ctttaccgtc
    24781 tgttcccacg ggccaacgag atggaaacct gcaacggtac gtttctcttc ctgcgtaatc
    24841 tcgtagttca tgacgcctcc attaacactg tttttatata cagcatagtt gcaatattaa
    24901 aaccacaagg aatgagtgtt gattatgcga gcagactcgc actcctgcca gtctgctgca
    24961 aaagaaaggt caggccttat ggtggaaata atcactcagg cgggaaaaca tgccgccttc
    25021 cccgacagat tccagggtaa ccagcggcca gtgcgccacc tgtttatcac ggtcgtaaag
    25081 ttcaatttcc cctacccgct gatgggcgct aattggcgcg gtgagctctt taccatcaag
    25141 ggtatatttg gctttgatat gtggaatttc ggctttcggt agcaccatcc agaactcttg
    25201 ttccgttcct aggtcgatat tttctttatc gccataccag atgcgttccg ttccgacctt
    25261 tttcccacgg tgcaaaattt gcaccgtagt aaagttttgt tgcccccaac gcagtaattt
    25321 tcttgcctct tcctcacgac cttttgcact gtcagccccc attaccactg caatgagacg
    25381 acgctgccca tctacagccg aagcaatgag attaaacccg gcaccagaag tatgacccgt
    25441 tttcaggccg tcaacattca tggttttatc ccacaacaac ccgttacggt tttgctgggt
    25501 gataccgttc caggtgagac ttttctcact gtacatatga taaaactcgg gctcgccgtg
    25561 gatgatagcg cgagaaaaca cagctaaatc ataagccgag ctatgctggc caggtgcatc
    25621 cagaccatgc actgtttcaa aatgcgtatc cttgagatgc agcttctcgg catagttgtt
    25681 catcatttca acaaactgcc gttgcccacc ggcaatatag tcagccagag caacacaagc
    25741 gtcatttccg gaatccacaa ttaaaccacg gcttaaatca cgtaccgata cgcgatcgcc
    25801 ctctttcaaa aacatcagtg aagaaccgac aaacaccgga ttatctttcg cccacgcatc
    25861 gcgccccacg gtgacaatat cgtctggcgt aatgcgatga ctatcgatag cgcgatccac
    25921 gacataaccc gtcatcagct ttgtcaggct ggcgggattg cgctgttgat gctcattacc
    25981 cgcggtgagg atctgaccgg tggtgtaatc catcaatacc caggacccgg catgaatctc
    26041 tggaggctga ggtgaaaaag gaatgttttc cgccgcaaaa ccagacgata agttaaaaac
    26101 gaacaaagaa gcagcaataa taagacggcg tttcaacagc aaaccctcag gagtttcaaa
    26161 tagctgttct ttttacggaa atacttatga actggctgga ataaagtgca agaaaatgtg
    26221 actaccctct catttttatc tgacatgatc tgttgccact cgctgccaaa ttgtggcgct
    26281 aaagctgatt agcacggtga tatttgatac tctggcagac agcagaaata acggatttaa
    26341 cctaatgatg aatgacggta agcaacaatc tacctttttg tttcacgatt acgaaacctt
    26401 tggcacgcac cccgcgttag atcgccctgc acagttcgca gccattcgca ccgataacga
    26461 attcaatgtc atcggcgaac ccgaagtctt ttactgcaag cccgctgatg actatttacc
    26521 ccagccagga gccgtattaa ttaccggtat taccccgcag gaagcacggg cgaaaggaga
    26581 aaacgaagcc gcgtttgccg cccgtattca ctcgcttttt accgtaccga agacctgtat
    26641 tctgggctac aacaatgtgc gtttcgacga cgaagtcaca cgcaacgttt tttatcgtaa
    26701 tttctacgat ccttacgcct ggagctggca gcatgataac tcgcgctggg atttgctgga
    26761 tgttatgcgt gcctgttatg ccctgcgccc ggaaggaata aactggcctg aaaatgatga
    26821 cggtctaccg agctttcgcc ttgagcattt aaccaaagcg aatggaattg aacatagcaa
    26881 cgcccacgat gcgatggctg atgtgtacgc cactattgcg atggcgaaac tggtaaaaac
    26941 gcgtcagcca cgtctgtttg attatctctt tacccatcgt aataaacaca aactgatggc
    27001 gttgattgat gttccgcaga tgaaacccct ggttcacgtt tccggaatgt ttggggcatg
    27061 gcgcggcaat accagctggg tggcaccgct ggcgtggcat ccagaaaatc gcaatgccgt
    27121 aattatggtg gatttggcag gagacatttc gccattactg gaactggata gcgacacatt
    27181 gcgcgagcgt ttatatactg caaaagccga tcttggcgat aacgccgccg ttccggttaa
    27241 gctggtgcat atcaataaat gtccggtgct ggcccaggcg aatacgctac gcccggaaga
    27301 tgccgaccga ctgggaatta atcgtcagca ttgcctcgat aacctgaaaa ttctgcgtga
    27361 aaatccgcaa gtgcgcgaaa aagtggtggc gatattcgcg gaagccgaac cgtttacgcc
    27421 ttcagataac gtggatgcac agctttataa cggctttttc agtgacgcag atcgtgcagc
    27481 aatgaaaatt gtgctggaaa ccgagccgcg taatttaccg gcactggata tcacttttgt
    27541 cgataaacgg attgaaaagc tgttgttcaa ttatcgggca cgcaacttcc cggggacgct
    27601 ggattatgcc gagcagcaac gctggctgga gcaccgccgc caggtcttca cgcccgagtt
    27661 tttacaaggt tatgcagatg aattgcagat gctggcacaa caatatgccg ataacaaaga
    27721 gaaagtggcg ctgttaaaag cgctttggca gtacgcggaa gagatcgtct aatttgaagc
    27781 ctccgccgct ggtacggcgg aggtcaatgt tgagtcaggc tttttgaacg gtgatactcc
    27841 acgctgcatc gccaatctgc tgataatcgg tgatggcatg cccttcttct gccgcccact
    27901 gtgggattgc ttcagttgcc tgggtgcaat caaactcaat taccaactca tcgccgctgg
    27961 ccatttctgc cagtgctgct tttgcttcta tgagtggaaa cggacagacc tgcgtcacca
    28021 catccagctt tttaatcgcc atctttacac cttacttaat ttgccgcagc agttgccagt
    28081 cgcgccttac gctgcggtcg aacatacaca agccaggacg cagtccagac tccgagaatc
    28141 ataaatacaa ggccaatcca gccctgccag gtcatcattg cagtcataac cagtccatta
    28201 ccgataaagc aaccacctgc aatactggca ccgaagccca tcagtacacc acctaacccg
    28261 ctacgtaatg ttgtttgtgc atcagctgcg cgaacgcgga actcacggct cgctttggct
    28321 gcaataaatg accccacgaa gatccctaac accaggaaaa cgccccagtt aatgtatttc
    28381 acgtcacccg cgaccagaaa ttgcagaata ttggccgttg gggaagtgat tcctaaccca
    28441 aacatgcgtc cggttgcttc actcaggggc caggctaaaa gcgcaatcaa accgatgagt
    28501 acagcagtga caaagggatg ccagcgtttt tcaaacagaa tatgagcgat cccagttcgg
    28561 cgcggcggta aggtcgcgac tttgagtttt ggcttcttca actctttcat caccacccag
    28621 agcgttatca ccagcaaaac ggcaaccaac ggccaaacag acaaattaaa agtctcagca
    28681 atagagttat gttcagtact gtagtgctgc aaggtttgat ttaaaccact ggcatgtgga
    28741 gaacgcatca ccgcactcat caccatataa gtgaaaagcg cgatccaact gccgatcaat
    28801 ccctcacccg cgcgatacca ggtcccggtg gcacatccac ccgccagaac aattcccagc
    28861 ccgaagatat acccacctat aacagtacca agccacggga acgcctcggc ttcgtaagtc
    28921 agtaggcccg cctgaattaa cgcaaaaacc cccacgcttt gtaccgaaat tgcaatcagc
    28981 aaggcgtaaa acatgcgatt atttttcgcg atatacatat cgcgaaaacc acctgtcagg
    29041 caaaaacgcc cacgctgcat gacaaatccc agcagagcac cacaaattag cccgcttaat
    29101 atcattgaaa acataaatta attaaccaga tgaatgttaa tgaggaaatt attcatgact
    29161 ggtggaatgc agaccaataa ccaaattctc taaattagaa caaatggtta ttaatgagga
    29221 tttcgataaa aaaaaataaa aaaaccggag ccaaatattc attcgactcc ggttgattat
    29281 tagaaaagat gattatgcaa cgtcttcgta ctgcggaact gggttacgga agctcttggt
    29341 gacgcaagcc aggtaaatca gaccgatagc agcccaaatc agacccagaa ccattgagct
    29401 ttcttccagg ttaacccaca gcgcaccaac ggtcagcgca ccacacatcg gcaggaacag
    29461 atactggaag tgatctttca gcgtcttatt acgcttctca cggatccaga actgcgagat
    29521 gaccgacagg ttaacgaagg tgaacgccac caacgcacca aagttaatca gcgctgtagc
    29581 cattaccagg tcgaagttga ttgccagcag ggcaatcgcg ccaaccagga tgatgttcat
    29641 cgccggagta cgccatttcg ggtgtacata accgaagaag cttttcggga atacgccgtc
    29701 acgacccatt acgtacatca gacgcgctac gcctgcatgc gccgccatac cggacgccag
    29761 tacggtaatg gtggagaaga tcagcgcgcc aacctggaac gctttacccg caacgtacag
    29821 catgatttca ggctgtgacg catccggatc tttaaagcga gagatatccg ggaagtacag
    29881 ctgcaggaag taagttgcaa agatgaagat catgccgcca atcagcgcgg tcaggaaaat
    29941 cgcacgcggg atcacgcgct ctgcatcttt ggtttcttcc gacaggttgc tgatgccgtc
    30001 aaagccggta aaggaaaagc acaggattgt cgccccggta atcatcggaa taacatgcgc
    30061 atcgccagac cagaacggac gagtgctcgc cagcgtacca gcgccttcac cttcaaatac
    30121 gccataaaca accatgccca gaatcaccgc gatcagcact acctgcaaca cgacgattac
    30181 agtgttgaag ttcgctacgg atttcagact acgcaggtta aaggcggtca taaaggccac
    30241 cagcgccacc acaaacatcc acgatgggat ggaaggcacc agagcttcaa aatagatttt
    30301 cgccagcaga atgttgatca tcggcgcgaa cagatagtcg agcagagaag accaacccac
    30361 cataaagccg acagtcgggc taatggattt ctgggcgtaa gtatatgcag agccagcaga
    30421 aggatagcgg cgaaccagct tcccgtagct cagagccgta aacaggatcg caatcaatgc
    30481 gaacgcatag gctgtcggca catgaccatc cgtgaggcct gaaacgatac caaaagtatc
    30541 aaacagcgtc atcggctgca tataggcaag acccatcatt acaaccggaa ctaacgtaag
    30601 cgttttacgc aattccacgc gagaggtgtt tggagtaacg ttatgcgaca tggtcattct
    30661 cctttacggt gataaccgtc gcgtaagcaa aaaattgccc catttttttg gattcctcag
    30721 cgacaacaac tgtcgatttt tagtaaatat ctatccggta cgaagcccgg cctcttggta
    30781 tgaatgattg gtttgaagca aaaaataacc gacgctgatg aaacgtcggt ttttagtcat
    30841 tttttgacag cggcgcattg tgcctaaatg ggggggaaat gacaagagaa tgagaagctt
    30901 gtcataataa tttttcttta aatggctgat tttccgtcat cagattttgc ataaacaccg
    30961 gcgatcgcac tatttgctaa aatttcatcc cgccaccagc cgctggcaag ccctgcggtc
    31021 tgttcgttcc agccaatcca gacgggttca aagagagttt gggcgaccac tttcttctcg
    31081 attaacgcgc cactatcgag aaaacgttgc accagataac ggggtaaata gccgcagccg
    31141 aggccgctaa tttgcagctc cagcttggtt ttaaaatcga aaacggtaat ggcttcttgt
    31201 tcgtcgagaa gttgtgaggc cgtagaagcg cctgcctgcg cagtgtcccc caccacaatc
    31261 gcccgatatc gcttaatgat acgacggttt aacggctctt cttcctgggc taacggatga
    31321 tgaggcgcga ccgcaaagac ttgttccaga tcgcccaacc gcgagaaacc aaactcactg
    31381 gaagatggtg gctcatgcat cgcgccaaca ataatatccg ctcgcccctg agtcagggca
    31441 tcccaggaac caccgagtac gccattgata aacttcaggc gcgtcacgct atgatgttga
    31501 taaaaagctt caatcagtgg cgcaagcaga gaaaaaggaa acgtgtcgtc cacgccaatc
    31561 accagctcgt tttcccagcc ttcatgcagt ttgatcgcct gcttttccag ttctcgcacg
    31621 gtatgcagaa cttcccgccc cttctctaat aacatttttc cggtacgggt gaatttagcg
    31681 cggtgaccgc tacgatcaag taattgaata ttaaggtcgc tttccagctt gtgaacggtg
    31741 taactcagag cggacggcgt cttgtagagt ttagccgacg ccgccgcaaa actgccttct
    31801 ttttctaagg catcgaggat catcaacaca tccagcagtt gtttcataca cgcccccttg
    31861 cagtgcatgc ggcgtcctgc tggcgtgatc gtttactcca gcggcattac cagcggatcg
    31921 ggatactggt attcaaatcc cagttcatta caaatccgac tgccatcaat aatcttacct
    31981 ttgccgctgt ccagactatt tctgaactgc ggaggttcca gccccagtaa acgggccatc
    32041 tgcggataga aaacattacg cgcagggtga gctggcgcac atatattata gatgtgtccg
    32101 cctttaggtg cctgtaacaa cagagtgata gcgccaataa catcttctaa atggactaaa
    32161 ttaacaccat gttcaccatc aggcgcggtt tttccggcaa agaagcgtcc gggatgacgt
    32221 cccggtccga ccagccccgc aagacgcaga atatcgaccg aagtaccggg taaattgtgc
    32281 agccagtctt cgagttcttc taatactcgt ccactgttgg tcaccggatt acgcggggtg
    32341 gtttctttca ccgtgccttg cgcgtcgcca tagacagacg ttgagctggt aaaaataatg
    32401 cgaggaatac gatgggccag cgcgctatcc actaactctt gtaccgcttg taaatagaac
    32461 tcatcgccgg ggccgctacg acgtgccgga agcgtaatga ccagcgcatc ggcatccatc
    32521 agggcatcca gatcgtcaga atcgcaaact aactcaggct ccatgcgaag cagatagcta
    32581 tcaatgccac tcattcgggc cgcttcgaca ccatcttgtg tggttttact cccggtgact
    32641 tgccagcctc gcgctgaaag tgacatcgcc agcggcatgc ctaaccaccc taacccgaca
    32701 attgcgacct ttttcatccg ttatctcctg atacacctta gatctataag gctacgctag
    32761 cgtatcaaaa ctgacaattc attctatgaa tgaatctgtt caataatgat aacgacatgc
    32821 tgcaatgagc agtgaatcgt cggtaaccgc gtataccaga cggtgctcct ctgtaatgcg
    32881 tcgggaccag aaacctgaca aattatgttt caggggttct ggcttcccct taccttcaaa
    32941 tggcgttctg cgggtatctt tgataagttc attgatcttt ttaacaattc gcttatctgt
    33001 ttcctgccag tacagataat catcccatga ttcctcagac cagattagtt tcactcaata
    33061 atgtcctttt ctgttccttt gcctgatttc aggctatcga ttgagtccat caatctccgg
    33121 gcgttagcgg gggagcgcag tagataagcc gtctcttcca gcgagttgta ttcttcgagt
    33181 gacatcagaa cacaagcctc tccattctga cgagtaataa ggatcggggc atgatcttca
    33241 acggctttca tcattgttgc cgacaaattc tgacgcgctt cgctgtagct aattgtacgc
    33301 atgtcaatct cctcttttgt acagtttatt gtacaatgat gggcgttaat taactattta
    33361 ttaattagtt tgtagatcaa ggtattgtca gtgagacgaa aatccaggct tcgctatttt
    33421 tggtgccgtc agcttagagg acagtcctct tagccccctc ctttccccgc tcattcatta
    33481 aacaaatcca ttgccataaa atatataaaa aagcccttgc tttctaacgt gaaagtggtt
    33541 taggttaaaa gacatcagtt gaataaacat tcacagagac ttttatgaca cgcgttcaat
    33601 ttaaacacca ccatcatcac catcatcctg actagtcttt caggcgatgt gtgctggaag
    33661 acattcagat cttccagtgg tgcatgaacg catgagaaag cccccggaag atcaccttcc
    33721 gggggctttt ttattgcgcg gttgataacg gttcagacag gtttaaagag gaataacaaa
    33781 atgacagaca acactcgttt acgcatagct atgcagaaat ccggccgttt aagtgatgac
    33841 tcacgcgaat tactggcgcg ctgtggcatt aaaattaatc ttcacaccca gcgcctgatc
    33901 gcgatggcag aaaacatgcc gattgatatt ctgcgcgtgc gtgacgacga cattcccggt
    33961 ctggtaatgg atggcgtggt agaccttggg attatcggcg aaaacgtgct ggaagaagag
    34021 ctgcttaacc gccgcgccca gggtgaagat ccgcgctact ttaccttgcg tcgtctggat
    34081 ttcggcggct gccgcctttc gctggcaacg ccggttgatg aagcctggga cggcccgctc
    34141 tccttaaacg gtaaacgtat cgccacctct tatcctcacc tgctcaagcg ttatctcgac
    34201 cagaaaggca tctcttttaa atcctgctta ctgaacggtt ctgttgaagt cgccccgcgt
    34261 gccggactgg cggatgcgat ttgcgatctg gtttccaccg gtgccacgct ggaagcaaac
    34321 ggcctgcgcg aagtcgaagt tatctaccgc tcgaaagcct gccttatcca gcgcgatggt
    34381 gaaatggaag aatccaaaca gcagctgatc gacaaactgc tgacccgtat tcagggtgtg
    34441 atccaggcgc gcgaatcaaa atacatcatg atgcacgcac cgaccgaacg tctggatgaa
    34501 gtcatcgccc tgctgccagg tgccgaacgc ccaaccattc tgccgctggc gggtgaccaa
    34561 caacgcgtag cgatgcacat ggtcagtagc gaaaccctgt tctgggaaac gatggaaaaa
    34621 ctgaaagcgc tgggtgccag ttcaattctg gtcctgccga ttgagaagat gatggagtga
    34681 tcgccatgag ctttaacaca atcattgact ggaatagctg tactgcggag caacaacgcc
    34741 agctgttaat gcgcccggcg atctccgctt ctgaaagcat tacccgcact gttaacgata
    34801 ttctcgataa cgtgaaagca cgcggtgatg acgccctgcg ggaatacagc gcgaagtttg
    34861 ataaaaccac ggttaacgcg ctgaaggtgt ctgctgagga gattgccgcc gctagcgaac
    34921 gcctgagcga cgagctaaaa caggcgatgg cggtggcagt aaaaaatatt gaaaccttcc
    34981 acactgcgca aaaactgccg ccggtagatg tagaaacgca gccaggcgtg cgttgccagc
    35041 aggtcacgcg cccggtcgct tcagttgggt tgtatattcc tggcggctcc gccccgctct
    35101 tctcaacggt attaatgctg gcgactccgg cgcgtattgc gggctgtaaa aaagtggtgc
    35161 tgtgctcacc gccgccgatt gccgatgaga tcctttatgc ggcgcagctg tgcggtgtga
    35221 aggacgtgtt taacgtcggc ggcgcacagg ccattgccgc gctggcgttt ggtacggaat
    35281 ctgtgccgaa agtggacaaa atcttcgggc cgggtaacgc ctttgtcacc gaagcgaaac
    35341 gtcaggtgag ccagcgtctg gacggtgcgg ctatcgatat gcccgcaggc ccgtcggaag
    35401 tgttggtcat tgccgacagc ggcgcaacgc cggatttcgt ggcttctgat ttgctttctc
    35461 aggctgaaca cggcccggac tcacaggtga ttttactgac gcctgacgct gatatggcgc
    35521 atcaagttgc cgaagccgtc gaacgccagt tagcagaact gccgcgtgcc gaaaccgccc
    35581 gccaggcact gaacgccagc cgcctgatcg tgactaaaga tttagcgcag tgcgtagaga
    35641 tctccaacca gtacggcccg gagcacctga tcatccagac ccgcaacgcc cgcgatctgg
    35701 tcgatggcat caccagcgcc ggttcggtat ttcttggtga ctggtcaccg gaatcggcag
    35761 gtgattacgc ctccggcacc aaccacgttc tgccgactta cggttacacc gccacctgtt
    35821 ccagcctcgg gctggcagat ttccagaagc gtatgaccgt gcaggaactg tcgaaagaag
    35881 gtttctccgc gctggcttcg accattgaaa cattggccgc cgccgagcgc ctgaccgccc
    35941 ataaaaatgc cgttactttg cgtgttaacg cccttaagga gcaagcatga gcaccgtgac
    36001 tattaccgat ttagcgcgtg aaaacgtccg caacctgacg ccgtatcagt cggcgcgtcg
    36061 tctgggcggt aatggcgacg tctggctgaa cgccaacgaa taccccactg ccgtggagtt
    36121 tcagcttact cagcaaacgc tcaaccgcta cccggaatgt cagccgaaag cggtgattga
    36181 aaattacgcg caatatgcag gcgtaaaacc ggaacaggtg ctggtcagtc gtggcgcgga
    36241 cgaaggtatt gaactgctga ttcgcgcttt ttgcgaaccg ggtaaagacg ccatcctcta
    36301 ctgcccgcca acgtacggca tgtacagcgt cagcgccgaa acgattggcg tcgagtgccg
    36361 cacagtgccg acgcaggaca actggcaact ggacttacag ggcatttccg acaagctgga
    36421 cggcgtaaaa gtggtctatg tttgcagccc caacaacccg acagggcaac tgatcaatcc
    36481 gcaggatttt cgcactctgc tggagctaac ccgcggaaaa gccattgtgg ttgccgatga
    36541 agcctatatc gagttttgcc cgcaggcatc gctggctggc tggctgacgg aatatccgca
    36601 cctggctatt ttgcgcacac tgtcgaaagc ttttgctctg gcgggccttc gttgcggatt
    36661 tacgctggca aacgaagaag tcatcaacct gctgatgaaa gtgatcgccc cctacccgct
    36721 ctcgacgccg gttgccgaca ttgcggccca ggcgttaagc ccgcagggaa tcgtcgctat
    36781 gcgcgaacga gtgacgcaaa ttattgcaga acgcgaatac ctgattgccg cactgaaaga
    36841 gatcccctgc gtagagcagg ttttcgactc cgcaaccaac tacattctgg cgcgctttaa
    36901 agcctccagc gcagtgttta aatctttgtg ggatcagggc attatcttac gtgatcagaa
    36961 taaacaaccc tctttaagcg gctgcctgcg aattaccgtc ggaacccgtg aagaaagcca
    37021 gcgcgtcatt gacgccttac gtgcggagca agtttgatga gtcagaagta tctttttatc
    37081 gatcgcgatg gaaccctgat tagcgaaccg ccgagtgatt ttcaggtgga ccgttttgac
    37141 aaactcgcct ttgaaccagg cgtgatcccg gaactgctga agctgcaaaa agcgggctac
    37201 aaactggtaa tgatcaccaa tcaggatggt ctgggaacac aaagtttccc gcaggcggat
    37261 ttcgatggcc cgcacaacct gatgatgcag atcttcacct cgcaaggcgt acagtttgat
    37321 gaagtgctga tttgtccgca cctgcccgcc gatgagtgcg actgccgtaa gccgaaagta
    37381 aaactggtgg aacgttatct ggctgagcaa gcgatggatc gcgctaacag ttatgtgatt
    37441 ggcgatcgcg cgaccgacat tcaactggcg gaaaacatgg gcattaatgg tttacgctac
    37501 gaccgcgaaa tcctgaactg gccaatgatt ggcgagcagc tcactaaacg agaccgttac
    37561 gctcacgtag tgcgcaacac caaagagaca cagattgacg ttcaggtgtg gctggatcgc
    37621 gaaggtggca gcaagattaa caccggtgtt ggcttctttg atcacatgct ggatcagatc
    37681 gccacccacg gtggtttccg tatggaaatc aacgtcaaag gcgacctcta tatcgacgat
    37741 caccacaccg tcgaagatac cggcctggcg ctgggcgaag cgttaaaaat ggctcttggc
    37801 gacaaacgcg gtatttgccg ctttggtttt gtgctaccga tggacgaatg ccttgcccgc
    37861 tgcgcgctgg atatctctgg tcgcccgcac ctggaataca aagccgagtt tacctaccag
    37921 cgcgtgggcg atctcagcac cgagatgatc gagcacttct tccgttcgct ctcatacacc
    37981 atgggcgtga cgctacacct gaaaaccaaa ggtaaaaacg atcaccaccg tgtagagagc
    38041 ctgttcaaag cctttggtcg caccctgcgc caagccatcc gcgtggaagg cgacaccctg
    38101 ccctcgtcga aaggagtgct gtaatgaacg tggtgatcct tgataccggc tgcgccaatc
    38161 tgaactcggt gaagtcagcc attgcgcgcc acggttatga accaaaagtc agccgtgacc
    38221 cggacgtggt gttgctggcc gataaactat ttttacccgg cgttggcact gcgcaagcag
    38281 cgatggatca ggtgcgtgag cgcgagctgt ttgatctcat caaagcctgt acccaaccgg
    38341 tactgggcat ctgcttaggg atgcaactgc tgggtcggcg cagcgaagag agcaacggcg
    38401 tcgacttgct gggcatcatt gacgaagacg tgccgaaaat gaccgacttt ggtctgccgc
    38461 tgccgcatat gggctggaat cgcgtttacc cgcaggcagg caaccgcctg tttcagggga
    38521 ttgaagacgg cgcgtacttt tactttgttc acagttacgc aatgccggtc aatccgtgga
    38581 ccattgccca atgtaattac ggcgaaccgt tcaccgcggc ggtacaaaaa gataacttct
    38641 acggcgtgca gttccacccg gagcgttctg gtgccgctgg cgctaagttg ctgaaaaact
    38701 tcctggagat gtgatgatta ttccggcatt agatttaatc gacggcaccg tggtgcgtct
    38761 ccatcagggc gattacggca aacaacgcga ttacggtaac gacccgctgc cgcgcttaca
    38821 ggattacgcc gcgcagggtg ccgaagtgct gcatctggtg gatctgaccg gggcaaaaga
    38881 tccggcaaaa cgtcaaatcc cgctgattaa aaccctggtc gcgggcgtta acgttccggt
    38941 gcaggttggc ggcggtgtgc gtagcgaaga agacgtggcg gcgttactgg aagcgggcgt
    39001 tgcgcgcgtg gtggttggct ccaccgcggt gaaatcacca gaaagggtga aaggctggtt
    39061 tgaacgtttc ggtgccgatg ccttagtgct ggcgctggat gtccgtattg acgagcaagg
    39121 caacaagcag gtggcggtca gcggctggca agagaactcg ggcgtttcac tggaacaact
    39181 ggtggaaacc tatctgcctg tcggcctgaa acatgtgctg tgtaccgata tctcgcgcga
    39241 cggcacgctg gcaggctcta acgtctcttt atatgaagaa gtgtgcgcca gatacccgca
    39301 ggtggcattt cagtcctccg gcggtattgg cgacattaac gatgttgctg ccctgcgagg
    39361 cactggcgtg cgcggcgtaa tagttggtcg tgcattactg gaaggtaaat tcaccgtgaa
    39421 ggaggccatc gcatgctggc aaaacgcata atcccatgtc tcgacgttcg tgatggtcag
    39481 gtggtgaaag gcgtacagtt tcgcaaccat gaaattattg gcgatattgt accgctggca
    39541 aaacgctacg ccgaagaagg tgcagacgaa ctggtgttct acgatatcac cgcttccagc
    39601 gatggccgtg tggtagataa aagctgggta tctcgcgtgg cggaagtgat cgacattccg
    39661 ttctgcgtgg cgggtgggat taagtctctg gaagatgccg cgaaaattct ttcctttggc
    39721 gcggataaaa tttccatcaa ctcccctgcg ctggcagacc caaccttaat tacccgcctg
    39781 gcggatcgct ttggcgtgca atgcattgtg gtcggcattg atacctggta cgacggcgaa
    39841 accggtaaat atcatgtgaa tcaatatacc ggcgatgaaa gccgcacccg cgtcactcag
    39901 tgggaaacac tcgactgggt acaggaagtg caaaaacgtg gagccggaga aatcgtcctc
    39961 aacatgatga atcaggacgg cgtgcgtaac ggttacgatc tcgaacaact gaaaaaagtg
    40021 cgtgaagttt gccacgtccc gctaattgcc tccggtggcg cgggcaccat ggaacacttc
    40081 ctcgaagcct tccgcgatgc cgacgttgac ggcgcgctgg ctgcttccgt attccacaaa
    40141 caaataatca atattggtga attaaaagcg tacctggcaa cacagggcgt ggagatcagg
    40201 atatgttaac agaacaacaa cgccgcgaac tggactggga aaaaaccgac ggactaatgc
    40261 cggtaattgt gcaacacgcg gtatccggcg aagtgttaat gctgggctat atgaacccgg
    40321 aagccttaga caaaaccatc gaaagcggca aagtcacctt cttctcgcgc actaaacagc
    40381 gactgtggac caaaggcgaa acgtcgggca atttcctcaa cgtagtaaat attgcgccgg
    40441 actgcgacaa cgacacgcta ctggtactgg cgaatcctat cggaccaacc tgccacaaag
    40501 gcaccagcag ctgcttcggc gacaccgctc accaatggct gttcctgtat caactggaac
    40561 aactgctcgc cgaacgcaaa tctgccgacc cggaaacctc ctataccgcc aaactgtatg
    40621 ccagcggcac caaacgcatt gcgcagaaag tgggcgaaga aggcgtagaa accgcactgg
    40681 cagcgacggt acatgaccgc tttgagctga ccaacgaggc atcggacttg atgtatcacc
    40741 tgctggtgct gttgcaggat caggatctgg atttaacgac ggtaattgag aacctgcgta
    40801 aacggcatca gtgagttgca gggtaagcgg atgcgatgtt gttgtcgcat ccggcaaaaa
    40861 aaaagggcaa ggtgtcacca ccctgcccgt tttctttaaa gctgcaaaga ttacttcgcg
    40921 ttataattac gcagagcgtt acgccccagc acaatccccg cgccaaccat gccgcccagc
    40981 agcaccgcca gaatcaaggt aattgccttt ttcgggctat cgcgacgaat aggtaacgtc
    41041 ggtttcatca cataacggta agcatgaata tcaagatcat caacctttaa gttttcaata
    41101 tccaacaggt tttgacgtgt ctgatagtag tttggtgaga acaccaacgg acgggtcgcc
    41161 tcatgcttaa tcatcgactc cagcgcttcg ctccccaaaa ggaacaacgt atcttgtgtc
    41221 acatcttcag tctgctgaac ctgtggcttt gccacctgcg cctgattcgc atactgcaac
    41281 gcttcctgaa tctgacggat acgcagatct ttctgctcct gcgcgactac ttcctgcgtt
    41341 cttaaagagt cctgcaagtt tttccgtccc agagcaatgt tgtctttgag atccttttct
    41401 agctcttgat tcactttatc atcaacttgc tgaatgtatt gggccaactt catttgtgct
    41461 ccctctgcag tttgcccaac ataagaaaca gtcaatggta attgctggtt cttaacagaa
    41521 ggttcgatgg taagtttttc tggttcttcc tgattatcca gcgtttctgc taatgcagag
    41581 aaggcagaac tgaagcgacc aattaacgtc tcctgtaaat ccgatacttt cggtgcagcc
    41641 tgaccataga taacattcat ggcattgtta tagccagcaa tttgccccac gtcgggctga
    41701 gtgataattg ctgttgacgt ccatttctcc ttcgctactg ccaaatatcc aatagccagg
    41761 gcaatagcca caatgacgga aatgatgatt gtcatcttgc cacgccacaa ctgcactagt
    41821 aaatcaatca aatcaatctg ttccgggtca tggttttgcc cagaaacatt attattttct
    41881 actctcatca ttaccctaac tgacggaaat ttccaagtcc gcatagttta gcgataatcc
    41941 tagtgaatgc tataggataa gtgataaaaa taatgaatta ttggggaggt aacaaaagac
    42001 aatcagggcg taaatagccc tgataacaag atgttagtcg ctgccaaaga gatcgcgggt
    42061 gtatacctta tctgccacat ctttaagctc ttctgccatt cggttagaga taatgacgtc
    42121 ggcttgttgt ttgaagatgg cgagatcacg ttccagccga gagttgaaga atgagtcttc
    42181 tttcatcact ggctcgtaga tgatcacttc aacgcctttc gccttgatac gcttcataat
    42241 cccctgaata gaagacgcac ggaagttatc tgaaccgctc ttcataatca gacgataaat
    42301 acccaccact tgcggtttgc gtgacaaaat ggcatcggca ataaaatcct tacgcgtgcg
    42361 gtttgcatcg acaattgccg agatcaggtt attcggcaca gactgatagt tcgccagtaa
    42421 ctgcttggta tctttcggca agcaataacc accataacca aacgacgggt tgttgtaatg
    42481 gttgccaata cgcggatcga gacaaacgcc ttcgataatc tggcgagtat tcagacctaa
    42541 actttctgcg tagctatcca gttcgttaaa gtatgccacg cgcatcgcta ggtaggtgtt
    42601 tgcaaaaagt ttaatcgctt ctgcttcggt cgaatcggtg aacagggtcg ggatattttg
    42661 cttaatcgcg ccttcctgta acaacgcagc aaaacgttct gcgcgttctg aacgctcacc
    42721 gatgacaata cgtgaaggat ggagattatc gtaaagggct ttaccttcac ggagaaattc
    42781 cggagagaaa ataatatttt cagtacgata tttcttatgc atcgcttcgg taaaaccaac
    42841 gggaaccgtt gatttgatga ccataaccgc ataaggattt atctcaacta cgtctttaat
    42901 tactgattct acactggatg tattgaaata attagtttta ggatcatagt cggttggagt
    42961 ggcgatgatg acataatcag catcccggta agcttcattt ttatctaatg tggcattaaa
    43021 gtgtatttta tctgattgca aaaactgctg aatttcctta tcaacaatag gagatatccg
    43081 atcattcagc atagcaacgc gtgacggtaa aatatctaat gccacaacct catgattttg
    43141 tgcgattaga agcccgtttg acaagcctac atagccagta ccggaaatgg tgattttcat
    43201 ttcatgctct cagaattaac ttaactgtga atcatgatgt ttttagcatc ctgataagag
    43261 ctaaatgttt taacgctacg ttattagctg gtcaacgcta cgaaaaacaa ttcagatttt
    43321 ctttataaga atagctgata tttattaagt taatattaag cagtgaaatt tagtctataa
    43381 gataaatggc aaaaaaaaag cccggtgcaa tatacgccgg gcctcaattt tactgttggt
    43441 taaatcagat taatccagcc attcagtatg gaacacacct tctttatcaa tgcgcttata
    43501 agtatgtgca ccgaaatagt cacgctgtgc ctggatcagg ttcgcaggca gaacagcggc
    43561 acggtagcta tcgtaatagg caaccgcagc ggcgaaggtc ggaaccggga taccgttctg
    43621 tactgcataa gcaacgacat cacgcagcgc ctgctggtag tcatcggcaa tttgcttgaa
    43681 gtacggagcc agcagcaggt tagcgatctg cggattttcg gcatatgcat cggtgatttt
    43741 ctgcaggaac tgcgcacgga tgatgcagcc agcacggaaa atcttcgcga tttcgccgta
    43801 gttcagatcc cagttgtatt cttcagacgc agcacgcagc tgagagaagc cctgagcgta
    43861 agaaacgatt ttgcccagat acagcgcacg gcgaactttt tcgatgaact cagccttgtc
    43921 gcctgctgac tgagcttgcg gaccagagag aactttagat gcggcaacgc gctgatcttt
    43981 cagagaagag atataacgtg caaacacaga ctcggtaatc agcgacagcg gttctccgag
    44041 atccagcgcg ctctggctgg tccatttacc ggtacctttg ttagccgctt catccaggat
    44101 cacatcaacc aggtagttac cgtcttcatc ttttttagtg aagatatctt tggtgatgtc
    44161 gatcaggtag ctgctcagtt caccgttatt ccactcggta aaggtctgtg ccagttcttc
    44221 gttagagaga ttcaggccgc ctttcaggag agaataggct tcagcaatca gttgcatatc
    44281 accgtattca ataccgttgt gaaccatctt cacataatgg cctgcgccat cagcaccgat
    44341 ataggtaacg cacggttcgc catcttcagc aacggcggcg attttggtca ggatcggcgc
    44401 aaccagttca taggcttctt tctggccacc aggcatgatg gacggccctt tcagcgcccc
    44461 ctcttctccg ccggaaacac cggtaccgat gaagttaaag ccttcggcag aaagctcacg
    44521 gttacgacga atggtgtcct ggaagaaggt gttaccacca tcgatgatga tgtcgccttt
    44581 atcgagatat ggtttgagcg aatcaatagc agcatccgtg cctgcacctg ctttcaccat
    44641 taacaggatg cgacgaggcg tttccagaga ttcaacaaac tctttcaccg tatagtaagg
    44701 aaccagtttc ttgcctggat tttcggcaat cacttcttcc gtcttttcac gggaacggtt
    44761 gaaaatagag acggtataac cacggctttc gatgttgagc gcaaggttgc gccccatcac
    44821 tgccataccg actacgccga tctgttgctt tgacattgtt tactcctgtc agggggtggt
    44881 caccgccgag tgtgcggctt aaatgtggta tagatgttaa ctcactcata gagatggtga
    44941 atagcattac ctttgctata ccacactgaa tatctcacct tttccgtctt ttaaaactta
    45001 tttatatgaa attactgaat tacttgttca gtaactcaag aataagtttt gtcttttctt
    45061 ccatcagcgg cacatcaccg cgagattcca cattcaaccg caccaccggt tcggtgttgg
    45121 aggtgcgcag gttaaagcgc cagtcggcaa aggtcatgct gatgccatcg gtgcgatcca
    45181 ccgccagcgc ctcgcggcta aaatgctgtt ccacgcggtt aatcgcctca aagggttgcg
    45241 ccagtttgct gttgatctca ccgcttgccg gaaacgctgc catccggtcg cgcaccagtt
    45301 cgcccagcgt ttttcctttc aggcacacca gttcggcgac cagcagccac gggatcatgc
    45361 cgctgtcgca gtaagcgaaa tcacggaagt aatggtgggc gctcatttca ccaccgtaga
    45421 tggcgtcttc cttgcgcatc cgttctttaa taaaggcgtg tccggttttc gacatcaccg
    45481 gcgtgccgcc tgcggcagtc accacatcaa cggtgttcca ggagagacgc ggatcgtgga
    45541 tgatcttcgc gccgggattt ttttcgagga atgcttctgc cagcaggccg acaatgtagt
    45601 agccctcaat aaactgccct ttttcgtcaa acaggaagca gcggtcaaaa tcgccatcaa
    45661 aagcaatgcc catatccgca ccgtgtttga tgaccgcatt gcgggtgtcg tcgcggcatt
    45721 ccggcagtag cgggttagga ataccgttgg ggaaattgcc gtccggcgtg ttgtgcactt
    45781 tgattaattc cacgggtgcg ccaagggctt taaagcgggc ttcgatggcg tccaccaccg
    45841 gacccgctgc gccattcccg gagttaatca ccagcttgag cggcgtgagg tttttgacat
    45901 tgatataacc gaacaggtga tcaacgtaag cgtcacgcag attgatttgc tgatagcgac
    45961 cgcgtttggt ttcatcaacg ggaggaaagt cgttggcttc tgccagacgc tggacatcgc
    46021 gcagtccggt atcaccgctg atcgggcgag ccccttcgcg caccagtttc atgccgttgt
    46081 aatccatcgg gttatggctg gcggtaactt caatgccgcc atccacgccg agatggaacg
    46141 tggcgaaata gatctcttcg gtgccggaca tgccaatatc cagcacatcg acgcccgcat
    46201 cctgtaaacc tctcgccagc gccagtttta aggtttcgct ggtgaggcgg acatcaccgc
    46261 ctaacacaat ggttttcggt ttgagaaatt cgccataggc gcgaccaatg cgccaggcga
    46321 tatcttcatt cagttcttcg cctaatttcc cgcgaatatc ataggcttta aagcaggtta
    46381 attttttcat gtcgttaccc tttttcaggc aatagttggc cctgaccgaa acggccaatt
    46441 ttgtttttgt cgttatttac atctcggttt cgcattcatt gcctgatgcg acgtttacac
    46501 ccgtccgtag cgatccgcga aacgcaccac atcatcctct tcgagataag agccggagcg
    46561 cacttcaatt aaatcgagcg gaattttccc cgggttttcc aggcaatgca tcgcccccag
    46621 cggaatataa atggactcgt tttcaccaag cagtttgata tcaccatcaa tggtgacttt
    46681 tgccgttccc gcgacaacca cccagtgttc cgcgcggtga tggtgcatct gtaccgacaa
    46741 gccctcgccc ggtttcacgg tgatgcgttt cacctggtag cggtcgcccg cgtcgataga
    46801 gtcatatttg ccccacgggc gatagacttc gcgatgcacc cgatgctcat ggcgaccatc
    46861 ggctttgatc tgctcgacca cttttttcac atcctgcacc gcgttacggt cggcaatcag
    46921 caccgcatct ttggtctgca ccactaccag atctttcacc ccgacggtgg ttacgaggcc
    46981 agattcggcg tacacatagc tgttttcagt tttgtgatta atcacatcgc cgtggcaaac
    47041 gttgccctcg gcggtgtggg cgctaatttc ccataatgag gaccaggagc cgacatcgct
    47101 ccagcccgca tccatcggca ccacaacggc atctgccgta cgttccatga ccgcgtaatc
    47161 caccgactct tccgggcagg cgagaaacgc ttcttcatcc acacgaataa aatcgagatc
    47221 cggatcgacg gcgctcatcg ctttttcaca ggcatcgaga atatccggac gatatttttt
    47281 cagttcttcg agatagcgtc cggcgcggaa caggaacatg ccgctgttcc agtaatattc
    47341 gccgcttgcc acataggcct gggcggtttc cagattcggt ttttcgacaa actgcgccac
    47401 ttcaaaggcc accgtatcct gctcacccgc agacacttca ccgcgacgaa tatagccata
    47461 accggtttct ggtagatccg gcacaatgcc gaaggtcacc agcttgcccg cttcggcata
    47521 tggcatggca ttacgcacgg cggcacggaa cgcgtcttca tcggcaatca catgatccgc
    47581 cgccaatacc agcattaacg ggtcgctctc cgggctatga cgttttgccg ccagcgccgc
    47641 cagcgcaatg gcaggtgccg tgtttcgccc tgccggttcg agaataatgt tctcggtaag
    47701 tttgttcagt tgacgcagct gttccgcgac aataaagcgg tgctgctcat tgcaaatcac
    47761 caccgggctt tcgcactcca cgccgttcag gcggcagatg gtggtttgca gcatggtgag
    47821 atcgcctttc aggcataaaa actgcttggg gtaaagtacg cgggaaagcg gccataagcg
    47881 gctaccggag ccacctgcca tcacaactgg atagagtttc gactgcgcca taattatccc
    47941 cgaatatcat ttataaattg acgtaacacg ttctctttat cgagtgtgcg ttcggcatat
    48001 tcacgtgcca ccgtgttgtg tttgggcagc aggagcgcct gacggatccc cgccaccagc
    48061 gcctcgaccg attccggttc tacgcaaacc gcaatgcccg gaaaggtttc gcaaagctgc
    48121 cccagttctg tgtgggcctc tgcggtaatc accgcgttac cgcctaccgc cagaatattg
    48181 gtcagtttcg acggcaatac ggcatctgcc gcgccgcgtt tttgcaccac cagatggcaa
    48241 tcgcccatct tcagcagtgc gggtaaagcg tcatacgatt gcagcgggaa aaattgcatg
    48301 ttgcgcagtc cacgctgctg cgccattttt tccagccgcg ctttgccgcc gccctgcccg
    48361 acaatggcaa aaatcagcgg ttcatcgcgc agacgatcgg cagcttcaat aacgttttcc
    48421 agcccctgct tttcaccaat attgccggag taaagaatga tttttttgtt atccggcagg
    48481 tcaagctggt tacgaagggc gtcaacatcg gcatctgcaa catgctgaaa acgggcaatt
    48541 tccgaccagt tggggaagaa gatgacgttt tccgccgcca cgcctttttc gatggcttta
    48601 ttcatcatcg aacgcgaaat cgtggagacg ttatcgacgt tatgcagtcc gctacgttcg
    48661 aacgccgttg ccagctgtgc cactttgctg cctttgcctt ttccggcaag gcccagcccc
    48721 agcatggcgt ccacttcgta atcctgaata tgcagcacgg tacgcgcacc agaaagtttc
    48781 gccagcaggc gcattcccgg cgtgcaaaac agcgttggca ccacgccgat aatgcgatcc
    48841 ggcttccagc gacgttgcgc catcagagga aagaaactgc tgacggcaaa actgccgaga
    48901 tgtaacaggc gtttcagggt gctcggctgt tttggcacgt acagcgggca gcgccacacc
    48961 gtggcggccc cctcttctcg tttgtaccgc caggcggaat agttctcgcc cacctgccac
    49021 tgcgggtagt aaggcggtgc ggtaataacc cgcacctcat gaccttgtgc cgccagccat
    49081 tccaccatct cgccggtgta tttgccgata ccggttaact ccggcgagta gttaatgccg
    49141 tagaccagta ttttcataat ccgggtactc cggcacgctt ctcagcgaga aaataggcgc
    49201 ggctgttagc gtgaacatcg ttactggcga gcagcgcgtc cggcgtcagc cagcggtaat
    49261 cgtcatgctg ctcatccggc agtatcagct cttcttcagc gactctgaag cgaaaaccga
    49321 gcaccacata gtgagtggtg aaatccgtgc cggagaagtt atcgtcataa aagtgctgcc
    49381 agacaccgta aaactggcct gctgttatcg gcagacgcag ccccagttcc gccatcgtca
    49441 gccgctcgaa tgcggcttcc agcgtttcgt ctttctgcac gcgccctccc ggcacaaacc
    49501 agtaaccctg cgccgggcgg ttggttcttt tgccaagcag aaactcaccg cgactgttct
    49561 cgacaataaa gtcgagagag acaagcgggg tggagcgcac taccgtggca aagtcttcct
    49621 gacgtaaaaa catcattacc cccgaaagcg gtcttgattc tcaaggaacc actggtaagt
    49681 gctggcaagc cccgcttcca gtgagatttc gtgataccag ccaagctgat gcaggcgcgt
    49741 cacatccagc agtttgcgcg gcgtaccatc cggtttgctg gcatcaaaaa ccacccgacc
    49801 tttgtaaccc accactttgg cgatggtttg cgccagctcg cggatagtgc agtcaacgcc
    49861 cgtgccgacg ttaatgtgcg acagcatcgg ctgggtgttc tccagccaga cttcgtgcgc
    49921 cagctccatg acatgaatgc tcgccgccgc catatcatcg acgtgcagga attcacgcat
    49981 cggtgtaccg ctgccccata ccaccacgtc cggtgcattc tgtgccgtcg cctcgtggaa
    50041 gcgacgcagc aatgcaggga tcacatgcga attactcggg tggaagttgt catgcgggcc
    50101 atacaggttg gtcggcatga ctgagcggta atcgcgtccg tactggcggt tgtatgattc
    50161 gcacagtttg atcccggcga ttttggcaat ggcgtaaggc tcgttggtcg gctccagcgt
    50221 gccctgcaat aactcgcttt ctgccatcgg ctgttttgcc agtttcgggt agatacagga
    50281 cgatccaaga aacagcagtt tgttcacgtc gttctgatgc gcggcgtgaa tgatgttgct
    50341 ctcaatcatc atgttctggt agatgaaatc cgccggatag gtgttgttgg caacaatgcc
    50401 gcccactttc gccgccgcca gatagacctg gtcaatgcgt tcgctggcaa agaaatcatg
    50461 caccgcgcgg ctgtccagca ggttcagctc gtcgcgggtg cgtaatacca gttccacatc
    50521 accgcgctgt tcgagctgcc gcctgatggc agaaccgacc atcccacgat ggccagcgat
    50581 aaaaatgcgt tgtttactca tgcttatgac tccagcgcga tcgccacgtc gtagccgtga
    50641 gatttcagca gagagtgttt tttcgccgct tcgaggtcat tagccaccat ttcagacacc
    50701 atctctctga gggtgatttc cggtttccag cccagttttt cgtgcgcttt ggtcgggtcg
    50761 ccgagcagcg tttcaacttc agccggacgg aagtaacgcg gatcaacggc gataatcaca
    50821 tcacccggtt taacgcccgg cgcgtcatgc ccggtgacgg aaaccacaat gcccttctct
    50881 tcaacacccg tgccttcaaa gcgcagtttg atgcccagct gtgccgctgc catttccacg
    50941 aactgacgca cggagtactg aacgccggtc gcgataacga aatcttccgg ctgttcctgt
    51001 tgcagcatca tccactgcat ttttacgtag tctttggcgt ggccccagtc acgcagggaa
    51061 tccatattgc cgaggtacag gcacgactcc agcccctggg cgatgttggc gattgcgcgg
    51121 gtgattttgc gggtaacgaa ggtttcgccg cggcgcgggg attcatggtt gaagagaatt
    51181 ccgttacaag cgtacatgcc gtaggattcg cggtagttaa cggtgatcca gtaggcgtac
    51241 agtttggcga ccgcatacgg agatcgcggg tagaacggcg tggtttcttt ctgcggaatt
    51301 tcctgcacca gaccatacag ttcagaggtg gaagcctgat agaaacgagt tttcttttcc
    51361 agaccgagga agcggatcgc ctccagcagg cgcagcgtac ccatcgcgtc gacgtcagcg
    51421 gtatattctg gtgactcaaa agagaccgca acgtggctca ttgcgcccag gttgtacact
    51481 tcatccggct gtacttcacg caaaatgcgc gtcaggttag aggtatcact caggtcgcca
    51541 taatgcagat ggaatttcgg gttgcaggtg tgcggatcct gataaatgtg atccacgcgc
    51601 tcggtgttga atgacgatgc gcgacgctta ataccatgca cctcgtaacc tttttccagc
    51661 agaaactctg ccaggtaaga accgtcttgt ccggttacac cggtgatgag agcgactttt
    51721 gacatgtctt attcctctgt atttttgaat ttattcagtt tcaacgcgtt cgcgtatcac
    51781 cactgcggga ttcccccggc aaaccacatt tgccggaagc gatttaaaaa cactgcttcg
    51841 tgcacccacg acggtgccgt cgccgattgt gacgcctggg gcaacaaaga catcggttgc
    51901 cagccagcat ttctcgccaa tcacaatagg cgtggcgtta atggtgaaat gttgacttgc
    51961 atggtcgtgg ctaccggtgc ataaataact tttttgcgat atcaccgaat gtgcgccaat
    52021 ggttatttca ccgagggtat ataaattgac gtcatcgccg acccacgcgt aatcacctaa
    52081 ggttaatttc cacggatagg taatttttac tgacggacga ataactacgt tttttcctat
    52141 ttttgctccg aataaacgta ataaaaaagc ccgccagcga tacaatactt gtggcgacca
    52201 ggcaaatatt gttgcctgta ctgcccacca taattgcact ttaatagcgt tgccgccccg
    52261 gaaccctttc ggcaccgaga aaccgcttaa atcttgcatt ggttttcctt atattcagac
    52321 tttgttatat aaggctttcg tctttgaggt agtacgttgg cgtaaatgcc aggataattc
    52381 agcccaaaag ccaggcacat gtaatatttg tcgttggact tttttcgcgt cagcacacaa
    52441 ttccatatta ttggtggtag ataccccacc catggaaaat tcagacacca ggccattgag
    52501 ttttttaaat gcataaccgg ctttatacat tttggctgcc agcgcgtagt cggaagaaac
    52561 tttatattcc aggtcataac gccatttttt caagccggat acagggaaaa atatcgcctg
    52621 atgactggcg ggcaggctgt gataaatata ccagcccggt ttggcgctac gtttaatttt
    52681 atgcccgtcg ccaaaatcca gcagcgcatc gccggtgatc atcatgttat ctttttgcat
    52741 ttttaactta cggacaaaat ttgcggcatc ctgatgaaaa atatcgcccg aattgagaaa
    52801 caacgcgaac ttgccttgtg ccatcgtaat gcctttgttc atggcgtcgt agataccgtt
    52861 atctggctcg ctgacaaagc gtaggttata gataccattg agattttcca gatactcacg
    52921 ggtgccgtca ttggaaccgc catcgacaac aatccattcg aagctgatat cttccgcctg
    52981 cgccagatgc gccagcgagg catgtgtttt gactatccct tcgaggttac gaaacgcgac
    53041 agtgattatg ctaagcaaca tgttcttatt cttacctcgt aatatttaac gcttttcgca
    53101 aaataaacgg acagacgatt aaaaatgcat attccgggct aaatatcgaa ccggtaaaaa
    53161 acagcgatac cggagtaaaa agatataatt gcacgcgaaa attgcggtta tcaccaaaag
    53221 cgttgatcat catttttatc actttcccca tgtaccacag cgataaaaac actgcgaacc
    53281 aggaaaaata aataatcagc agatacaaac cattgtctat ggtttttccg acatccgcac
    53341 cgttaaatat tccgaatgat gcgacgtatt cataaagtga gccaaatctg actacaccgt
    53401 caatatgggt caaagaataa ccgaccatca ccagcggacc gacaatacga taatacgatg
    53461 acgatccttc cgtacctaaa tcgcccagac gggtggaaat ataaggaaac gcgattacca
    53521 caccaacccg gaatacagcc agagaaatca atgccaacgg taactttttc ttaatcgcct
    53581 ctttattcag atattgaaat gcccactcca gcaaataaaa caggataaaa gtcataaccc
    53641 ctgaaaacga tccggataat attatccctg cgagaatcat agcatcggtt ttaggcgttt
    53701 tgataccaaa ctgtttgatg ctgagccaaa ttgagattaa tgccagagcg aaaaatgccg
    53761 gttcgaaata cagtgctgtc gtgcgcttgc cgccgaattt aatgaaattc agtacatagc
    53821 tgttgctgta aatcagatat ttcgaaatcg actccataat actgctgcca ccggtgagga
    53881 taatttgcgc catctccacc gccgccagcg ccaccaccag cccgatcacc agataaaaga
    53941 aacgtaatat cttgcgatgg ttgtgtggcg aaatcgtttt aaagcgaata ctccacacca
    54001 tgccaataat gatcacaata tagacaaaca gcatggttga agtgacgtat ttactggcat
    54061 ccagcgactg accaaacaga tagttaaacg ccgtgagtcc cgcaccaatc cctaaggcaa
    54121 tcatcaattt tttaacgctg atgcgttcta aaaacagcag taacaagaca ggtaaaaaag
    54181 tgacgatggt gatggggaaa ctttcgccaa gctgggcgat tttgacgtta accagcaggt
    54241 agatcagcgg cagcagcagg tagctacaga ttctgataga agttgacata ctcctccagc
    54301 atctgttgtc cactgtaggt ggcgcggctg cgttggctga actcagccag cgtggtacca
    54361 aatatcgcct gcgcgatttc cggtttgctt aactgcacca gttgcagcac gtcttcttcg
    54421 tcgacggttt taccgccgga tttttgcaac acttcccgtg ccgcgtcgct atgggtggca
    54481 atcaccggca cgccaattga taacgcctca cacaaaatca gcgggtagtt atcgacgcga
    54541 gaactgaata ccagcgcatc catctgattg agcgcgctca tcaacttgcg cttgtcagtt
    54601 tcaaagccgt gattaaccac gttgccagcg gtgaacggcg agaacttacc aaaggtatgc
    54661 agttcgattt tgtcgcccag cgccatcatc tcacgcacca gttgctggtt agtcttgccg
    54721 tcgtaacgca agtcatgcgc caccaccgcg attttcggct tgccctgggt ttcgcgcacc
    54781 ggaggcaagt ccgccagaat cgcttcggtt gccatatcaa taccattatt gataatccgg
    54841 caacgccctg gaccgtacag gctattgaaa gcgtcagcca catgctggct gggggaaata
    54901 aactgacagc ccagcgccag catctcacgg aataactggc gtttgcccgc caccagctgg
    54961 tgtgcgcgat caatcttcac cggcggatag ttatttaagg tcgggcattt ctggcagccc
    55021 gttttccagc cttcgcaacc gtcggtaaag gcgcagcgtc cggtaacact ccagtggtcg
    55081 tgcagcgtcc agaccagagt gacgtccggt ttatggtttt tcactttttc gcaaaagcgc
    55141 accacgctct taagatttag ccagtagctg tgcagcacat gaaaatgcag gaccaccgga
    55201 cccggtgtgc gagtaatggt gcgatataac tcattgaaat tgccaaacag atcgcgatta
    55261 aacagacgaa acagggcaat attcgccatc gcggtcatcc gcggcgtatg tttgatgacc
    55321 tgcggatagt tctgatggct gacgctctct ttgccgcctt tgccataacc gtagacaaaa
    55381 tgtgacgcca gcccctgttg cagcgcacgc tggtggagat ctaacgccac acctgctgcc
    55441 ccgccttccg ccagtcgcac attaaattgc agaatattca tttaattacc ttcactcgcg
    55501 ctttttctcc caccaccagc gcgttgtccg ggacagagtc gagcaccacg ctgcccgcac
    55561 ccacggtgac gttgttacca agcgtgatat caccaagaat aatgacgttg gcaccgagtt
    55621 cgacgccgtt gccaatgtgt ggacatgcca tgttatcggc accacgattg ccgatagtga
    55681 cgccgtggcg aatggtgaaa tcatcccccg ctaccacgtt tttattgatc actacggcgt
    55741 aaccgtgatg gatagtaaag cggcggccaa tggtcgcggc ggcctggatt tcataaccga
    55801 aaaagcattc ggtgataatg cgatacagca ccagcagcgg ggccgcccac agattgttga
    55861 ggacgttctt tttgcgccac accgaacaaa aatgagcaac acgataggca agaaccatgc
    55921 agcacgggcg taaactccag ctgttggcac gcagatcttc cagcatcttt agcgcccccg
    55981 aataccatca gccagacgtt tgccgttacg caccgacagc agcgtcagca aagtgcgcca
    56041 ggtcatacgt ttattgcgga tctggtagag ggtaaacagc tgatattttt tgcttgcacg
    56101 gtcaaattta tctttgtgct ttcggtaaaa gtggaagtac ccggagaatt ttttcggtga
    56161 cgaggtgatc tgcatttcac cgtgattgat atgcaggatc tgcgtcgctt cttccacttt
    56221 ccacggttcg ccgtactcca ccaccatccg caggaagatg tcgtaatcct gtgctgcttt
    56281 cagttcggta tcgaacaaac actctttgaa acgccatgcc caagtaaaga cctgattgcc
    56341 aatgatattg cgtttataga acaggcggcg tgagtacggc gatttgggat acagtggtaa
    56401 gcttgccggt tgggaataaa cttctccctg gcatacgtag tcgttagcgt acaaaaaggc
    56461 gtgagtgacc agttgctgtt tatgggcgag gaagacgctc agacggttgg gtgtccattc
    56521 gtcatcgtca tcgatcccgg taatgtattc cccttgcgcc agcataatcg cctggttacg
    56581 taccgcgcac gccccgctgt taatgtcgtt gtgaatgtag gtgatacgcg gatcgttgag
    56641 ggcagtgacg tactgttgca gttgttccca ggaggtggag caatcatcca cgatgatcat
    56701 ctcccagttg ctgtagtcct ggcgcaggac cgattttatc gcccgaatcg ccagttgttg
    56761 acggttccag gtcggcatat agattgaaat cagcggattc tctttcatag gttgttctcc
    56821 cggcttatga gctggtttgc ttgccggatg cggcgtgagc gccttatccg gcctacgggg
    56881 cggtgcgaat gcaggcgtcg ttatttcgca tccgacttat attcgtattc gtaatagcca
    56941 taatcctgat acgcgctggc gcggcggaag atggagttca gaatcacccc tttcaccgga
    57001 ataccgtttt gctcaaagcg gctcagactg gtttccactt ctttcaatgt gttgaccgca
    57061 tagcgcgcca ccattaacgt ggttccagcg tgacgcccga caattgctgc atcggtcact
    57121 gccagaatcg gcggcgtatc aatcaacacc aggtcgtagt ttttactcgc ccagctcacc
    57181 aattcggcaa agcgttcgct catcaacagt tcagaaggat ttggcggcac ctgaccgcgc
    57241 gggatcaggt caaatttggc aatagaggtc ggtttagctg cggtagtaat atcgccctgg
    57301 ccaatcagaa tttccgacag gccattgacg ttattggtac ccaacagctc gtgggtgtag
    57361 cctttgcgca tatcgcagtc gatcaacaac acccgtttat tggtctgact gatcaccgcc
    57421 gccaggttgg cgcagacaaa ggttttaccg attgacgggc tcacccctgt catcatcaac
    57481 acattgttct gcgcctgcat catcgcgaag tgcagactgg tacgcagact gcggatggct
    57541 tcaatcgcca gatcggttgg attccccacc gccagtagct ggctctgttt atagcgttta
    57601 atccctttga tggttttgac gctatcgcgc gctttctgcc attccgacag cgggatgctg
    57661 gcatagacgc tgataccatg ttcttccagc acctgcgggc tttcgatgcc gcgattaaac
    57721 aacgagcgca gcagcacacc cacgatagag agcatcaggc cgaggataat tgcgccgagg
    57781 ataatcagtc ctttcttcgg tttcagcacg ccaggctggg tgattgccgg gtcaacaatg
    57841 cgcacatcgc cgacggtgct ggcttcggtg attttcagct cctgctcttt attcagcagt
    57901 tgcatataga cctgctgacc agactcgaca tcgcgggtca ggcgcacaat ctcctgctgg
    57961 gttttcggca tcgccgttac gcgaccgtta agtttggctt tctcttcttc cagcgcctga
    58021 cgtttctcca gcagcgtgcg gtacgccggg tgaactttgg tgtacagctt ggagatttcc
    58081 gcctctttaa aggtcagttc gttcaactgc gcgtcgatgt tcaccatcga atcgagcacc
    58141 gctttcgctt ccagcggcag atcaacagaa tctttatcct gacggaaggc attcagtttg
    58201 ttttcggcaa catcgaggcg gctacgtact tccggtaact gttgcgcgag gaaagcgagg
    58261 cttttcgacg cttccgccga tttgcgctca atattttgtt cctgatagtt acgggcgatg
    58321 ctgttaagaa tgtcgcggat ctgttcgcga tcttcaccgg tataagtcag gctcagtacg
    58381 cctgcgtctt tgccgttctc cgttaccgtc aggctgtttt gcagttggtt gatcatcccc
    58441 aacgtggagt atttggtgac ggtaaactca ctgcccgggc gggcgtgaat ggcttcaacc
    58501 atcagcgtga cgccttcttt tttcagcatc tggcccgctt gcccacgggc gctaaagccg
    58561 ccatcactgc tcagggtgta gtttttattg tccagcacat taagcgtaaa cacctggccc
    58621 gccatctctt tcgggcggtt aaaggtagtc actttcaccg tctcgttctg gcgtcccatc
    58681 agtcgctccc agcccgcccc gaaaatcggg aacgtgtttt tgctcactgc aatatcgagg
    58741 tcgagatcgt ccaccgtttt accaagcacc agacgcgaac gaatcaactg gatctcggcg
    58801 tccgatgcag gcggtttgtt ggctaatgcc gaaccaatgt cctgcactaa cgaattgccg
    58861 ctgttttgct cgatttgtac cagtgcgtcg gcactataaa tcggcgttgc gaagaaggtg
    58921 taaaccacgg cacagagggc aaatacagcg gtgatgccaa tcacccacca gcgcgcttca
    58981 atgacggtgc cgaccaggcg accaatatcg atttcatcac tgcccgttac cggagcggca
    59041 tgttgtttta ctttttctgt cattcttata cctgctctgc gttcaatgcc tgcgcccact
    59101 ggcgggcaga ccgttcaagt aatgtgtaca ccgctgcaaa cgtttcccgg cttttgcgat
    59161 acggatcggg gatttcacat tcgttatccc agtgaccaaa cagcatcact ttgccgcgca
    59221 tctcaggtgc catctcgcat aagcgttcga tatggcgctt ttccatggtc aaaatcaggt
    59281 catagttgcg acacaggcgg cggctgattt gtcgtgcaca gtggccttcc agagacagtt
    59341 gatgttctgc ggcgacgctg atagcggtag gatcagcgcc cttaccgacc agcgcgccga
    59401 gtccagcgga ctccactttc agttccgggt gataacgttg cagtaagcgt tccgccgtcg
    59461 gggaacggca aatattgccg acacagacaa ctaatatgtt gttaaacatg acgattacca
    59521 gttatgaatg tcgctggctg tatccgtcat gtaacggaca ccgctaatag ttggcaacaa
    59581 ctgattgatc agacggttcc agcgggaaac cggggcggtg gtgacataca ccacgtcata
    59641 aggttgcagg cggaattctg tcgccatcac cagcgacgtg gcatcggaca tatccagctg
    59701 gtagatattg gcaatcttgc cattacgccc gccctcgcct ttcagcggac gaatgacaaa
    59761 gatgccgctg gcgttggagg tggtcatgtc gataccttca gcattaccca gggcttcagt
    59821 cagggtcatg ccgctaaagt ccattttcag ggtgctctgt ttcttcactt cacccatcac
    59881 aaatactttc agatcatcat tacgcggcac gtagagaata tcgccgggat aaagcagtcg
    59941 gttctgattg aggtcgccgt tttgcatcag cgcctgcaaa gaaatgcgtt cttcacgacc
    60001 attgtgtgtt agcactacgt tgcgccagtc agcggtgtcg gtcaggccac ctgcggcgtt
    60061 gatggcgtcg agaatagtca gtggcacgtt ggtgatcgcc tgttgaccgg atttattcac
    60121 ctgaccggag atataggcct tttgtgagcg gaaggcggcg atattaacgt ccacctgcgg
    60181 gtcagcgatg tacgtcgcta agcgcccggt aatatcactg cggatttcag cgagcgtttt
    60241 cccgactacg tgaaccttgc cgatatacgg gtaaaacata gtgccgtcag gctgtaccca
    60301 gttgccggtg tcgctggagc tgcggtactg accggctggc gtggtgagtt ccgggtgatc
    60361 ccagacggtg acattcagaa cgtcgcccgg cccgacgcga tactggtaat tcgcgatctc
    60421 gctttccagc gtcatattgg ggcgcgctac attcgggcgt gggcgtaatt ggtcaatcag
    60481 gcgcggggtc agcggataaa cattcaccat tttgtcgaga tcgaaatcag cgtcctgctg
    60541 tttgatgacg tctttgccca tcgtcgacat attgctgccc ggaagtactg tgcaaccgct
    60601 tatcaaggtt actgacacca ataatggcat caatttcatt ttggatttca tcattgttta
    60661 tttatcactt tggcagagta attatcctgt gcactattaa tagcaatatc gccatgcaca
    60721 tttaccttgc agttaattga ataaaaattt aactggcatc agtcctaaaa aaattgattt
    60781 catccgcagg ctattgacag aataattcag actggtcttt caggcatcca gacacgctac
    60841 cgcccctggc tttttagcca ccaacacact gatttagctt aatttttcaa accctctctg
    60901 catacaatcg ttgatgagaa agggttattg cggaaattaa cttccgaata taaggtgaca
    60961 ttatggtaat tgaatattgg ctctccaata atgcaggagg aagtgttaca gctaacggaa
    61021 tagcaggcaa ggtaacaatt cggcaattgg ctatttttaa gaattatatt aagtgtcatt
    61081 caatatggtt tttaggagtt tctttaggtt gacaatattt aatattgcgc ctccacatgc
    61141 gatatttctt aaataatgtt ttattattac cacttttaat tcagggataa tgtgaggtta
    61201 ttaccctcaa ataatatgag ataaatagtg ctgcaacatt gcatttttgc cccgatttat
    61261 ccatgatcga attgtgacat ttgtcataca acgaataggt tttgtactta ctatggaatg
    61321 gattgccgat ccgtctatct gggccgggtt aatcacgctg attgtgatcg aactggtcct
    61381 cggcattgat aacctggtct ttattgccat cctcgccgaa aaactaccgc cgaggcagcg
    61441 cgaccgcgca cgggttaccg ggctgctgct ggcgatgtta atgcgcctgt tactgctggc
    61501 gtcaatctcc tggctggtca ccctgactca accgctgttc agcttccgct cgtttacctt
    61561 tagcgcccgc gacttaatca tgctgtttgg tggtttcttc ctgttgttca aagccacgat
    61621 ggagctgaac gaacggctgg aagggaaaga cagcaataat cccacacaac gcaaaggtgc
    61681 gaagttctgg ggcgtggtga cgcaaattgt ggtactggac gccattttct cactcgactc
    61741 agtgattacc gccgtcggga tggtcgacca tttactggtc atgatggccg ccgtggttat
    61801 cgctatcagc ctgatgttga tggccagcaa gccgttaacg caatttgtta acagtcaccc
    61861 gacgatcgtt attctctgct taagcttcct gttgatgatt ggctttagcc tggtggcaga
    61921 aggtttcggc ttcgtcattc cgaaaggcta cctgtacgct gccattggtt tctcggtgat
    61981 gatcgaggcg ctcaatcagt tggctatctt taaccggcga cgttttcttt ccgctaacca
    62041 gacgctgcgc cagcggacta ccgaagcggt aatgcgcctg cttagcgggc aaaaagaaga
    62101 tgcggaactg gacgccgaaa ccgcgtccat gctggtggat catggtaacc agcagatctt
    62161 taatccgcag gaacggcgga tgattgagcg ggtacttaat cttaaccagc gtaccgtcag
    62221 cagcattatg acgtcgcgcc acgatattga gcatatcgat ctcaacgcgc cggaagaaga
    62281 aatccgccag ttgctggagc gaaatcagca tacgcggctg gtggttaccg acggtgatga
    62341 cgcagaagat ttgctcggtg ttgttcacgt tatcgacctg ctacaacagt ctctgcgcgg
    62401 cgaaccgctc aacctgcggg tgttgattcg ccagccgctg gtgttcccgg aaaccttgcc
    62461 gttgttacct gccctggagc agttccgtaa tgcccgcacg cactttgctt ttgtggtgga
    62521 tgagtttggc tcggtggaag ggattgtgac attaagtgac gtcactgaaa ccattgtcgg
    62581 taacttaccg aacgaagtgg aagagatcga cgcccgccat gatattcaga agaatgcgga
    62641 cggttcctgg acggcgaatg gtcatatgcc gctggaagat ctggtgcaat atgtgccgct
    62701 gccgctggat gaaaaacgtg aatatcacac cattgccggg ctgttgatgg aatatttaca
    62761 gcgtattcca aaacctggcg aagaagttca ggtgggggat tatttgctta aaacgttgca
    62821 ggtggaaagc catcgcgtgc agaaggtgca gattattccg ctgcataagg atggcgagtt
    62881 ggagtacgag gtgtgatggt atcggtcatt ttacccaata gccgaaagga ataagatgag
    62941 ggaaccgcgc cagcggcgat ttaccgccgg gagcccgggt tgccagggtg gtggcgattg
    63001 agccaccctg gcacgttcac cggcttgagc gttccatagt agcgaggaac atcaagtgaa
    63061 cggaacagcc ttcgatctta catattcaga atataatgat gccgccacga acgcctgatg
    63121 cgacgcttta cgcgtcttat caggcctaca ggactggtca cctggtaacc aacatcttct
    63181 ccagcaactt cttcacatcc ttgccattgc gggaatcttt attccgctcg gcccagtcat
    63241 tcaggcgacg tttcgcttcg tcctgtagat gtttgcgcag taactgatcc acttgcaggc
    63301 tgtaattgag ttgctgccag ttgccataaa cccgcagcgg tactggcgtt tctttcagga
    63361 aatcaatcag tttactttcc ccgttccagc cacccacgac ccgaatatca aactgggtat
    63421 cgcaggtttg atctgccaga ttcaacatgc cttcccctgt cagcgccagc acaggcgatt
    63481 gaccttgcat gtcgtttaac gtcacgacgc catccttcaa cgtcaaatcg gtggtaaagc
    63541 ggtcaagccg cgtcacgtta tcaaagtttt cagcggcctt cacatcaccg ccattacgct
    63601 ctaccgcttg ctgaatcatc tgctggaagt tcatcccttc catgcgcgtg tcggtcattt
    63661 cgacatgtgc ctgtccttgc cagttgtggc ggaatgcgtc ggcatctatg tcagcaccgg
    63721 agaaatcacc agccagtgac atttttccgg tcaacgaaat cggatagtta aacgccttca
    63781 gaatggtacc aatctcaacg ttttccagcc gtggctggaa gtttatccgc ggatttattg
    63841 atgtcgcgtc cagcgtgccc ggcagtgaaa cctgtccacc gttaagtttg ccctgcagtt
    63901 gagtaatttc cagcaaaccc gacttgttgg tcatttgcgt ggcaacatct gtaaaattca
    63961 ttccgcgcca gcgcacgtta ctggcctgca acaaaatatc agccgtaaag ccttgcagtc
    64021 cctgataggc cggttcatca atacgcgaag aaatgaccgg gcgcggcaac gttgattggc
    64081 tctgcccctg ctgcgcggca ccgttttcac cattcgcggt ttcattaagc gggatgaggt
    64141 tgtcaagatt cagttgcggg aattgcagtc tcagctgcca ttccggtttc tctgtcagcg
    64201 tgacctgtgc ttgcccgctc agcgtactgt cattggcggt caagctgatt tggttaaatg
    64261 aaaggcgttt atgtgactcc tgccactggg cctggaaact cccctgcccc tgaatacctt
    64321 gttttggtaa atcggcaccc tgcaactgcc agttaatttg ttcaatagcc gccgttaaat
    64381 catgcggata atcagaagca tccaccgtac cgttaaggga tattgtgaga tcgcgctgat
    64441 cgcgattaac ccgcccggag aactcaaatg agccacgatg ttggggatct tgttccattt
    64501 gcaggcggat attgcggatt gtcacctgct cgtcatcttc atgctggaac accagcacgc
    64561 tgtccgccac cttaagactg gatatatcaa acgaccatcc gcgatcgtct gacagatccg
    64621 gcaaggtatt gtcgcgcggt gcaaccggag cgtcttcact gcgcaccgct tccgtctgcg
    64681 gcgtcagttg gatcactgcc ccttttagca tcacctgctt aacgctcagt tgatgactca
    64741 gtagtggtaa aagcgccacg tccagacgca tgttgtcggc gcgaaccagt ggctggcttg
    64801 cgccctgggc ggtgagagac attcgcccgg agaggatact aagctgcggc cagacgtgcc
    64861 aacgcagtgg cccgtcgagc tgcaattgat aaccgctacg tgcagcaact tgcttgacca
    64921 tatagtcgcg gaaatcattc ggattcacca gcaacactaa cacagataac ccggccacca
    64981 gcacgaccag gagtatcatc agcgtcgtca gaaatcgtct catggtatcc tcaatgggcc
    65041 tgaattagtc tttatcgatt cggctggcta ccgcgccctg ctggttgcga tatttcgcat
    65101 cttcacggcg gttgtaaggt cgcgccgccg ggccggaaag cggctcaaag ctcagcgcac
    65161 caattaacat gcccggacgc agcgccagcg gcagcttacc ggagttgtag aactccagca
    65221 caatgcaacc agaccagccc ggatcgatac ggtgcgcggt gacgtgcacc atcagcccca
    65281 gacgcgccaa agaggaacgc ccgtccagcc agcccaccag atcggctggc agcgtcaccg
    65341 actccagcgt caccgccagc gccagctctc ctgggtgaag atagaacgcc tcgccctcgt
    65401 cgagaacgat ctcatcgctc atcacgcggt caagcgcggc gctcacttca tctttgggac
    65461 cgctcagatc gataaacgct gccgtgtgac cacggaaggt acgaaattta ttgcccaagc
    65521 gtacatccac cgtcgcgccg ttaatacgct ccactggcgg acgtgggttg atcgacaaac
    65581 ggccttcatc aagccaggct tcaatatctc ggtcacacag acgcatggca ttttctcctt
    65641 tcgcgcatca ctcccttaac gccaattacg tcaagggcat accaggttat cactgaacgg
    65701 tacacaattt atcaagctta ttcaaagaac tgacttattt tcgctttcaa tatatcgatc
    65761 gcgatgcggt ttttcccgcc gcgcggcacg ataatgtccg catattgttt agaaggctca
    65821 atgaattgca ggaacatcgg gcgcacggtt ttttgatatt gcgccatcac ggaatccatt
    65881 gaacgcccac gctcgttaac gtcacgcttg atgcggcgca tcaggcagat atccagcggg
    65941 gtatcaacga aaatggagaa gttaagttcg tcacgcaaac gcgcatccgt cagcaacaaa
    66001 atgccttcga gaatgatgac cttcttcggc tcaaccgtca ccgtttcttt catacgcgta
    66061 tgttcaacat agctgtaaac cggcaggtca attgccgagc cgcgtttcaa cgcttgtaaa
    66121 tgctcaagca gcaggctgtg atccatcgcg ctgggatggt cgtagttggt cttaacgcgt
    66181 tcttccatcg acagatggct ttgatctttg taatagcagt cttcgggaat tacgccgatg
    66241 tgttcatcac cgacttgctc acgtaattca cgataaaggg tactggcaat aagactcttg
    66301 ccggaagccg atgcgccagc gataccgata atgacgcact gatgagactg atcagtcata
    66361 tatttagcga cctgattaac ctggatgtta ggaagggggc gacgaagcgc caaacgcggc
    66421 aattataggg atttcatccg tctgatacca gtcgaatagc gttgccgcgc gctcagagtt
    66481 aattgttgac aaagaattcc cggtggcaaa ttacgttgat cagttttatg taaggtaaaa
    66541 aatgttatac gcagttgcgc aaattatccg cctttacgtc actttatgag caattcgcat
    66601 ataaaatgta aaacttttgt actagcataa acacagaaac gaatactggc gacctggtct
    66661 tgcggataaa gcggtaatga gcaaacaatc acagcatgta ttaattgccc tgccccaccc
    66721 gctgcttcac ctggtcagtt taggtttagt ctcgtttatc tttacccttt tctcgcttga
    66781 gctttcgcag tttggcaccc aactcgcccc actgtggttc ccgacgtcca tcatgatggt
    66841 ggcgttttat cgccatgccg ggcgcatgtg gccgggaatt gcgctgagct gttcgctggg
    66901 aaatatcgcc gcatccatcc tgcttttttc caccagctcg ctgaacatga cctggacgac
    66961 catcaatatt gttgaagccg tggtcggggc agtgctgcta cgtaaattgc tgccgtggta
    67021 taaccccttg caaaatctgg ctgactggct gcgtctggca ctcggcagcg ccattgttcc
    67081 gcctctgttg gggggtgttc tggttgtcct gctgacgccc ggagacgatc ctctcagggc
    67141 atttttgata tgggtactgt cagaatccat cggcgctctg gcactggtgc cgctgggatt
    67201 gttatttaaa ccacactatc tgctgcgcca tcgcaaccca cggttgcttt ttgagtcgct
    67261 gctcacgtta gccatcacac tgacgttaag ctggctttcg atgctgtatc tgccgtggcc
    67321 ttttactttc attattgtgc tgttgatgtg gagcgccgtg cgcctgccac gaatggaagc
    67381 ctttttgatc ttccttacca cggtgatgat ggtgtcgctg atgatggccg cggatccctc
    67441 cctgcttgct acgccgcgta cgtacctgat gagccatatg ccgtggctac cgtttttgct
    67501 gatcctgctg cccgccaaca tcatgaccat ggtgatgtat gcctttcgtg cggaacgcaa
    67561 acacatttcc gaaagcgaaa cccgctttcg gaacgcgatg gaatattccg ctatcggtat
    67621 ggcgttagtg ggcaccgagg gacaatggct gcaaaccaac aaagcgctct gccagtttct
    67681 cgggtacagt caggaagagc tgcgcggact cacctttcag caactgacct ggccggagga
    67741 tctcaataaa gatctccaac aggttgaaaa gctgataagc ggtgaaataa acacctattc
    67801 aatggaaaaa cgctactaca accgcaatgg cgatgttgtc tgggcgttgc ttgccgtctc
    67861 actggtgcgc cacacggatg gcacgccgct ctattttatc gctcagattg aagacattaa
    67921 cgagctaaaa cgcaccgaac aggtcaatca gcaactgatg gagcgcatca cgctggctaa
    67981 cgaagcgggc gggattggca tctgggagag ggagttgaag ccgaatgttt ttagctggga
    68041 taagcggatg ttcgagctgt atgaaattcc tccacatatc aaaccgaact ggcaggtgtg
    68101 gtacgagtgc gtgctgccgg aagatcgcca gcatgccgaa aaagtgattc gtgattcgtt
    68161 gcaatcacgc tcgcccttta aactggaatt tcgcattacc gtaaaagacg gtattcgcca
    68221 tatccgcgcc ctcgccaacc gggtactgaa taaagaaggc gaagtcgaac gtctcctcgg
    68281 catcaatatg gatatgaccg aagtgaaaca gcttaacgag gcattgtttc aggaaaaaga
    68341 gcgcctgcac attacgcttg attccatcgg tgaagccgtg gtctgtattg atatggcgat
    68401 gaaaattacc tttatgaatc cagtggcgga gaagatgagc ggctggacgc aggaagaagc
    68461 gttaggtgtt ccgctcctga cggtgttgca tattactttt ggcgacaacg gaccattaat
    68521 ggagaacatt tacagtgccg acacctcacg ttccgcaatc gaacaagatg tggtgttgca
    68581 ctgtcggagc ggcggcagtt acgacgtgca ttacagtatt acgccgttaa gtactctgga
    68641 cggcagcaat attggttcgg ttctggtgat tcaggacgtc accgaatcac gcaaaatgct
    68701 gcgccagctg agctacagcg cctcccatga tgcactgacg catctcgcca accgcgccag
    68761 ttttgagaaa caactgcgta tcctgctgca aacggtaaac agtacgcatc agcgacatgc
    68821 cctggtgttt atcgatcttg atcgctttaa agcggtgaat gacagcgccg ggcatgcggc
    68881 gggcgacgct ttgctgcgcg aactggcgtc gttgatgctg agtatgctgc gctccagtga
    68941 cgtgctggcg cggctcggcg gtgatgaatt tggtctgctg ctgccagact gcaatgttga
    69001 aagcgctcgt tttatcgcta cacgtattat cagtgctgtg aatgactatc actttatatg
    69061 ggaaggccgg gtacatcggg taggtgccag cgccgggatt accttgatcg atgacaacaa
    69121 tcatcaggcg gcagaagtga tgtcgcaggc tgatatcgcc tgttatgcct ccaaaaatgg
    69181 tggacggggc cgggtgacgg tttacgaacc acagcaagct gccgcacata gcgaacgggc
    69241 ggcgatgtcg cttgatgaac agtggcggat gattaaagag aatcagttga tgatgatcgc
    69301 ccacggtgtc gcttcgccgc ggatcccgga agcgcgtaat ttgtggctga tttcgcttaa
    69361 gctctggagt tgcgaaggcg aaattattga tgaacaaaca tttcgtcgta gcttcagcga
    69421 tccggcactt agccatgctc ttgaccgacg ggtattccac gaatttttcc agcaggccgc
    69481 caaagcggtt gccagtaaag gcataagcat cgccctcccc ctttccgttg ccggtttgag
    69541 tagcgccacg ctggtgaatg atctgcttga gcagctggaa aatagccctc taccaccacg
    69601 gttattacat ctgattattc cggctgaagc gattttagat cacgcagaaa gcgtgcaaaa
    69661 actgcagctg gcgggatgtc ggatagtgct cagccaggtg ggccgcgatc tgcaaatctt
    69721 caactcgctg aaagcgaata tggcagatta cctgctactt gatggtgagt tatgcgccaa
    69781 cgtgcagggt aatttgatgg atgagatgct gattacgatt attcaggggc acgctcagcg
    69841 actcgggatg aaaaccatcg ccgggccagt cgttttaccc ttagtgatgg atacgctttc
    69901 tggcatcggc gtcgatctga tttatggtga tgtgattgcc gatgcccaac cgctggattt
    69961 gctggtgaat agcagttatt tcgcgattaa ctgagagagc gtttgcccat tgcctgatgc
    70021 gacgctaacg cgtcttatca tgcctacaaa tcgctcattc cccagaccgg ataaggcgct
    70081 cgcaccgcat ccggcgacca acgtcatgct tcgtctggtt gccagccttc cgtataccag
    70141 atatgcaaca gcgcataaga acgccagggc ttccagcgct cggcataacg gcggatttgt
    70201 gccggtgtca ttcccggaaa tcgccgttta atcagataat catccggcag aaaaacatct
    70261 ttcgcctgcc agccacgcaa agcaaaataa ttcgccgtcc agcgcccgat acccggaaaa
    70321 gtttgcagcg ttttcatcgc ctgctccaca tcgcccggaa ttgtcattgg taagctgccc
    70381 tccagcgccg catttgccag atgaatcagt gcctctgccc gtttcaacgg catgcctaac
    70441 gcttttaatg cctgcgggtc ggctgctgcc agccgctgag gcgtggggaa gcagatatac
    70501 tccggaaaat catccagccg ttcgccataa agctgtacca ctttggcagt caattttgcc
    70561 gccatcgcca cgctcaccag ttggcctaaa atcgcccgca caccctgctc aaaagcatca
    70621 atacagccgg gtaaacgcaa tccgggccgc cccgcgccta actttcccaa cgcaccgtta
    70681 acaatctgcg ggttacattg cagatcaagc aggcggctca ttttcgccag acactctgcg
    70741 gcaacaggtt ctaaacctgc acttaaattt atgtgcagag tatggcgggc tatatccgga
    70801 atagcagtca ctacgccgcg atattcgccc accgccagac tacgggcata ataattgtcc
    70861 gcgaccgttt ccacaccgct caccgcacgg gcggcgagaa atcccaacat ccacgaccag
    70921 tcatacggcg gctgccagtt cagggtatac atcgcatctc cttgttaatc cgctttcagc
    70981 ataaacgtta ttcagacgtt acgctttgct ttcatattcc ggttgtcgcg acagcaacat
    71041 ttcgctaaag tcacgcccct tcttcaccgg catggggatt aattcgtgtt tattggtttt
    71101 gattacggta cagcaaactg ttcagtggcg gtcatgcgtg acggtaaacc gcaattactg
    71161 aaaatggaaa acgacagcac gctgctgcct tcaatgcttt gcgcgccaac gcgtgaatcg
    71221 gtaagcgaat ggctgtaccg ccatcatgat gttccggcag acgacgatga aacgcaggcg
    71281 ctgttacgtc gggcgattcg ttataaccgc gaagaagata tcgatgttac ggcgaaaagc
    71341 gtgcagttcg gtctttcctc actggcacag tacattgatg atccagaaga agtgtggttt
    71401 gtgaaatcac caaaatcgtt cctcggtgcc agcggcttaa aaccgcagca ggtagcgctg
    71461 tttgaggatc tggtctgcgc aatgatgttg cacattcgcc agcaggcgca ggcgcagctg
    71521 ccagaagcga ttactcaggc ggtgattggt cgcccgatca acttccaggg gctgggcggc
    71581 gatgaagcaa acgcccaggc gcaagagatt ctggaacgcg cggcgaagcg tgccggattc
    71641 agggacgtgg tattccagta cgagccgatc gcggctgggc tggattacga agccaccttg
    71701 caggaagaaa aacgggtgct ggtggtggat atcggcggtg gtacgactga ctgttcattg
    71761 ctgctgatgg ggccgcagtg gcgttcgcgt ctcgatcgtg aagccagtct gctgggtcac
    71821 agtggttgtc gtattggcgg taacgatctg gatatcgccc tggcgtttaa aaacctgatg
    71881 ccactgctgg gtatgggtgg cgaaaccgaa aaaggcatcg ccctgccgat cctgccgtgg
    71941 tggaatgcgg ttgccatcaa cgacgtacct gcgcagagtg atttctacag tagtgccaac
    72001 ggtcgtctgc ttaacgatct ggtacgcgat gcccgcgaac cggaaaaagt ggccctgtta
    72061 cagaaagtct ggcgtcagcg tttaagctat cgcctggtgc gtagcgcaga agagtgcaaa
    72121 attgctcttt caagcgtagc ggaaacccgc gcctcactgc cgtttatcag caatgaactg
    72181 gctacgctga ttagccagcg agggctggaa agcgccctca gccagccact ggcgcggatt
    72241 ctggaacagg tgcaactggc cctggataac gcccaggaaa aaccggacgt tatctacctg
    72301 accggcggta gcgcccgttc tccgcagatt aaaaaagcgc tggcagaaca gttgccgggc
    72361 attccgattg caggcggcga tgactttggc tccgtcaccg ccgggctggc acgctgggcg
    72421 gaagtggtgt ttcgttaatt gaaaaatggc tcagcagcgg cttaaatctg cctgcgcctg
    72481 agccagtttt tctgctgcct gacgtaacgg ttccatcaac ggcatggtcc gtgctggagc
    72541 atgaaggcgg aatcgctgca actctgtcgc gccgactgtc aacgccgatt ccagccgatg
    72601 tcgtttggcg gtcattttcg ccattaccgc ttgtctgtct gctggcgtta tcgcttcatt
    72661 cggcctgaac acaaaacggc gttcacagtt cgctttccag tcgataaccg cctgggtcag
    72721 atggtcgcca aaccctttca cttgcttaac gccacgacgg gtaacatctg cggctgtttc
    72781 aataccaaaa gaccgtaacg ccgctttacg cgcagggcca acgccgggaa tagaggcaac
    72841 atcaataaaa aatccctcca gaaacttctg cttctgccgt tcccttgcag tgtcatgaag
    72901 tgctgccaga gcgcgtttct cttcttccgg taacccgaga atttcgtcct tcattttttc
    72961 gagcatcgtc cgtttggcga taaaaccttc caggccgcct acctgctgga tctggcggac
    73021 taaatggtca tagtccattt tcgcacgatt aaatgcctca cggcgttgct gaacttctgc
    73081 tttgtacgct ttgcttgtca aactgccaat aatccagata gccgccagca caggaaccaa
    73141 aataatataa cgcggttctg cctgaaggcc gcaaagcagc agcgataatg ctgacagtgc
    73201 gatctcaagc agaatgatgt attcgcgccg taacaggcct aaaggaagcg gcctgccagt
    73261 cggttggaaa tgatcgggta atggcagttg caatgctggc ggtgctactg acgccatcac
    73321 catcgcccag acttttgcca gcacaaaatc cccgccggtg gtaatgacct cttcgccgag
    73381 atcaataaaa tagataacac cttgattatc cagcgcacac catgggcagt cagccaaatg
    73441 accgggataa acatgcattg ccgaaacggt acatttcttt aactgttggc gtagagaatc
    73501 cagtgctgat acccacgctt tagccgtcgg acgcccggtt gccacgccac tttccgtaaa
    73561 cgcctgctga aacatggctt caacatcact cggtaacatc gatagcggaa tcgatcgtgg
    73621 cggcggtttt aaaccacgtc gctgattatc tgacgcgtag gcataacgga aatgggtaat
    73681 atccgtctcc agcgcattac ccgcatcaga gataagcggc acaccagaat aaggatgccg
    73741 gccaccaaac aagacgtgaa aaatcagcaa cgcaaggcca aaattatcgt gattttctgt
    73801 gcgttcaaag ccgacaaatg acgacaaggt ttgtagctct ggcggcgtaa aatgcgacac
    73861 gccgacttcg cataaatgca gtgtgccatt ggcgttaatc tgaaaggagt cgctatcaat
    73921 caacaccact ttgctgtcgc gacctaccat aaaactgttc tggtttacgt cacccacgac
    73981 gtgcccgtgc tcgtgaaccg tagcaaaaga tgaagcaata ttgcgcgcaa catagagtag
    74041 aaaatcccac gcacaatgag ggtaacgttg acgacgatgt gccgggctat agatcatatg
    74101 aatcggttct ttaccagaaa cttttggcat cataaaaccg ataacttttc cgcctcgtcc
    74161 accgtgaagc gttgcctgcg gccaggcgac ataattcaac aattgcgcgt cagctgtcgc
    74221 agccataaag gcaagtttgt cctgttttaa ggcgggtggc ggcgtgtgat aaatcttggc
    74281 gacgctatcg acaaactcat tgatatcata aaccgcgcct tcaccgcctt tgcccagttc
    74341 acggcctggc gtaacgcact caccagtagc agtatataaa gtgggtttca tggtaaccac
    74401 agcgccagtg ccaacgtttt atcatcatcc gtacgctcgt ttaccgccgg gctggacaaa
    74461 aactgcttta acaattcagg taataaatcg agttgttcct gcgttgctga cgccagtcca
    74521 ttgaaaaacg gggtaaagaa aggcacatga ggagaattat ccagcatatt taacgccagc
    74581 cgctgaatac catccgtaaa tgctgcaact ttatgcgcac gcccagtgct ggtgaaagtt
    74641 tccagtctgg aaacggcatc ttcgtcggta atgaagtgcg tcatgttggc atattcaccc
    74701 accatcggcg tgagaggcaa ttgcagaccg tgacccaaat ctaccaccac gccgccatcg
    74761 ccaatctgca taatcaaggt gccgtcaggc gatgaaatca atcccagaaa cgtgcaggca
    74821 aaatcgcgaa ctgctaatgc tttggcttct gcctcggcaa aaagtcgttg acgaatagtc
    74881 agtaccatat ttgtggcgag gacatcatta agccccaatt caccgtcctg tactttttgc
    74941 accataaacg ccatcgcttc attgacggcg agcgtcgcgc cttctcctcc ctgcgaaacg
    75001 ctacctgcgc catcagcaag gaacatcacc agtaatggtt gctggtcgtt taaccagaca
    75061 acctgcatct ggcaggcatc ctgacaaggc aaatcagcac taatgtgcga tgttccaaca
    75121 gcggatgcat aaaccagacg ccagctcaca ctgacgtcca gccttttggc gcttccagca
    75181 caacttccgt tcccggcgtg gagcgggaaa ccgaacgcag cgaactggat aaccagctaa
    75241 acagttcacg gaattgtaat ccctgcagtg gcaaaggctg acgaacgctg atttgtgcca
    75301 gagtcttcat atccgccccc tgaacaccga tggaaaagaa ggcaaacttc ttatcttctt
    75361 ccccctgaaa cactttgttg gcggcagcct gccactcatc ggttggagcg ccatcggtga
    75421 tcaggaaaat ccacgggcga taatacgaaa taccatttgc gcggtactca cgtttcctct
    75481 cttcaaccat atccagggct ttggtaatgg cggcccccat aggcgtatcc ccctgggcaa
    75541 acaggatggg cgggaaaaag ttagcggcgc tggtaaaggg ttgttccaca tgcaccgggc
    75601 cgaacgtcac aatgccgagt tccactcttt ttaaagccag cggatcagca agcagttcat
    75661 cacgaaaggt aaccaatccg gcgttaagtt cattaatagg tctgccgctc atcgagccag
    75721 aaacatccag cagcaaaatg caggggcaac gtggttccgg gttactggca aaatcgctgg
    75781 tggcaaacgt gatttgttcg ctcataagaa atcctttctt agagaattat cgaggtccgg
    75841 tatcataccg attgcaataa tgaaataaga gcaaacgaag aaactttcag aattaggccc
    75901 gtcggcgcag aaaattttgc tattcgcctc gcaagaacgg agagccgtcg attgacacta
    75961 agggcggagt gacataattt caggagtgag ggttagggag aggtttcccc ctccccctgg
    76021 tgttcttagt aagcctggaa gctaatcact aagagtatca ccagtatgat gacgtgcttc
    76081 atcataaccc tttccttatt aaaagccctc ttctccggga gagggtttcc cgtttcagcg
    76141 tcccgctgaa atcgtcggct tacctccttt cgccctgaat gcagtctatc gctaacgcgt
    76201 gaacaaaata gtttcatctg tgattattgc gtaacgactc gcaaagaata tttgctggca
    76261 ggatcgcaga ctacaaagcc tgcggattga caatcttatc gtgaaggcat aatttcagga
    76321 gtgagggtag agcggggttt cccccgccct ggtagtctta gtaagcgggg aagcttatga
    76381 ctaagagcac cacgatgatg agtagcttca tcatgaccct ttccttatta aggccccttc
    76441 ctcgggaggg gctttcccgt ttcagcgtcc cgctgaaatc gtcggcttac ctcctttcgc
    76501 cctgtatgca gtctatcgct gacttgcgga taaaatagtt tccagtgtga ttattgcgta
    76561 acgtctcgca aagaatattt gctggcagga tcgcagactg caaagcctgc agattgacaa
    76621 tcttatcgtg aaggcatact ttcaggagtg agggtagagc ggggtttccc ccgccctggt
    76681 agccttagta agctggccag ctaatgacta agagcaccac gatgatgagt agcttcatca
    76741 tgaccctttc cttatttatg gccccttcct cgggaggggc tttcccgttt cagcgtcccg
    76801 ctgaaatcgg cggcttacct cctttcgcca tgcaagcagt ctatcgctaa cgcgtagata
    76861 aaatagtttc ctgtgttatt actggatgcg tgctcgcaaa tgtgcccgtc attcagacga
    76921 ttcccgacag tgtttcataa ttcctccatt tttctccctt attggctggc tacactagta
    76981 tcattccgcg aaacgtttca ggaagagaaa ctcttaacga tgaaaggcag ttataaatcc
    77041 cgttgggtaa tcgtggtggt tatcgccgcc atcgccgcat tctggttctg gcaaggccgc
    77101 aatgactccc agagtgcagc cccaggggcg acgaaacaag cgcagcaatc gccagcgggt
    77161 ggtcgccgtg gtatgcgttc cggcccatta gccccggttc aggcggcgac cgccgtagaa
    77221 caggcagttc cgcgttacct caccgggctt ggcaccatta tcgccgctaa tactgttacg
    77281 gtgcgcagcc gcgtggacgg tcaactgatg gcgttacatt tccaggaagg ccagcaggtc
    77341 aaagcaggcg atttactggc agaaattgac cccagccagt tcaaagttgc attagcacaa
    77401 acccagggcc aactggcaaa agataaagcc acgcttgcta acgcccgccg tgatctggcg
    77461 cgttatcagc aactggcaaa aaccaatctc gtctcccgtc aggagctgga tgcccaacag
    77521 gcgctggtca gtgaaaccga aggcaccatt aaggctgatg aagcaagcgt cgccagcgcg
    77581 cagttgcaac tcgactggag ccgcatcacc gcaccagtcg atggtcgcgt tggtctcaag
    77641 caggttgatg ttggtaacca aatctccagt ggtgatacca ccggaattgt ggtgatcacc
    77701 cagacgcatc ctatcgattt agtctttacc ctgccggaaa gcgatatcgc tactgtagtg
    77761 caggcgcaaa aagccggaaa aacgctggtg gtagaagcct gggatcgcac caattcgaag
    77821 aaattaagtg aaggcacgct gttaagcctc gataaccaaa tcgatgccac aaccggtacg
    77881 attaaagtga aagcacgctt taataatcag gatgatgcgc tgtttcccaa tcagtttgtt
    77941 aacgcgcgca tgttagtcga caccgaacaa aacgccgtag tgatcccaac agccgccctg
    78001 caaatgggca atgaaggcca ttttgtctgg gtgctgaata gcgaaaacaa ggtcagcaaa
    78061 catctggtga cgccgggcat tcaggacagt cagaaagtgg tgatccgtgc aggtatttct
    78121 gcgggcgatc gcgtggtgac agacggcatt gatcgcctga ccgaaggggc gaaagtggaa
    78181 gtggtggaag cccagagcgc caccactccg gaagagaaag ccaccagccg cgaatacgcg
    78241 aaaaaaggag cacgctcctg atgcaggtgt tacccccgag cagcacaggc ggcccgtcgc
    78301 gcctgtttat tatgcgtcct gtcgccacca cgctgctgat ggtggcgatc ttactcgccg
    78361 ggattatcgg ttatcgcgcc ctgcccgttt cggcgctgcc ggaagtggac tatccgacca
    78421 ttcaggtggt cacgctctac ccaggtgcca gcccggatgt catgacctct gccgttaccg
    78481 cgccgctcga acgccagttc gggcagatgt ctggcctgaa acagatgtcg tcgcaaagtt
    78541 ccggcggtgc gtcagttatc actttgcagt tccagctaac attaccgctc gatgtcgccg
    78601 agcaggaagt gcaggccgcg attaacgctg cgaccaactt gttgccgagc gatctgccta
    78661 acccgccggt ttacagcaaa gtgaacccgg cagatccgcc gatcatgacg ctcgccgtca
    78721 cctcaaccgc catgccgatg acgcaagtgg aagatatggt ggaaacccgc gtcgcgcaga
    78781 aaatctcgca gatttccggc gtcggcctgg tgacgctttc cggcggtcag cgtccggctg
    78841 ttcgcgtcaa acttaacgct caggcgattg ccgccctcgg cctgaccagc gaaaccgtgc
    78901 gcaccgccat taccggcgct aacgttaact cggcaaaagg tagcctcgac ggcccttccc
    78961 gtgcggtcac gctttccgcg aacgaccaga tgcaatccgc cgaagagtat cgccagctaa
    79021 tcatcgccta ccagaacggc gcgccaattc gtctgggcga tgtcgcaact gtagagcaag
    79081 gtgcagaaaa cagctggctc ggcgcgtggg cgaacaaaga acaggccatt gtgatgaatg
    79141 ttcagcgcca gcccggtgct aacattatct ccaccgccga cagcattcgg cagatgctgc
    79201 cacagctcac tgagagtctg ccgaaatcgg tgaaggtgac agtgctttcc gatcgcacca
    79261 ccaatatccg cgcatccgtc gatgatactc agtttgaatt gatgatggct atcgcgctgg
    79321 tagtcatgat tatctacctg tttttgcgca atattccggc gaccatcatt cccggtgttg
    79381 ctgtaccgct gtcgttaatc ggcactttcg cggttatggt gtttctcgat ttttcaatca
    79441 ataacctgac actgatggcg ttaactatcg ccaccggatt cgtggtcgat gacgccatcg
    79501 tggtgatcga aaacatttcc cgctatatcg aaaaaggcga aaaaccgttg gcggcggcgc
    79561 tcaagggcgc aggtgaaatc ggctttacca ttatctcgct gaccttctca ctgattgcgg
    79621 tgttgatccc actgctgttt atgggcgata tcgtcgggcg actgttccgc gaatttgcta
    79681 ttaccctggc ggtagcgatt ttgatctcag cggtggtgtc gctgaccctg acaccgatga
    79741 tgtgcgcgcg gatgctcagc caggagtcgt tgcgtaaaca gaaccgcttc tcccgtgcct
    79801 cggaaaaaat gttcgacagg ataatcgccg cctatggtcg tggactggcg aaagtgctga
    79861 atcatccgtg gctgacctta agcgtggcac tcagcacgct gctgcttagc gtgctgctgt
    79921 gggtgttcat tccgaaaggt ttcttcccgg tacaggacaa tggcattatt cagggcactt
    79981 tgcaggcacc gcaatccagc tcctttacca atatggccca gcgacaacgc caggtcgcgg
    80041 acgtgatttt gcaggatccg gcagtgcaaa gcctgacctc atttgttggc gttgatggca
    80101 ctaacccgtc gctgaatagt gcacgtttac aaatcaacct caaaccgttg gatgaacgtg
    80161 atgatcgggt gcaaaaagtc atcgcccgtc tgcaaacggc ggtagataaa gtgccgggcg
    80221 tcgatctctt cctgcaacca acgcaggatc tgactattga tactcaggtc agccgcaccc
    80281 agtaccagtt taccttgcag gccacctcac tggatgcgct cagtacctgg gtgccacagt
    80341 tgatggaaaa actccagcaa ctgccacagc tttctgatgt ctccagcgac tggcaggacc
    80401 aagggctggt ggcgtatgtc aatgttgatc gcgacagcgc cagccgtctg gggatcagca
    80461 tggcggatgt cgataacgcc ctgtacaacg cgtttggtca gcgactgatt tccactattt
    80521 atactcaggc taaccagtat cgcgtagtgc tggagcacaa caccgaaatt accccgggcc
    80581 tcgcggcgct ggataccatt cgcctgacca gtagcgacgg cggcgtggtg ccgttaagct
    80641 caattgccaa agttgaacag cgttttgcgc cgctttccat caaccatctg gatcagttcc
    80701 cggtaacgac catctccttt aacgtgccgg ataactattc gctgggcgat gcggtgcagg
    80761 agattatgga caccgaaaag acgctgaatc tgccggtgga tatcaccacg caattccagg
    80821 gcagcaccct cgccttccag tcggcgctgg gcagcactgt ctggctgatt gtcgcggcgg
    80881 tggtggcgat gtatatcgtg ctcggcattc tgtacgagag ctttattcac ccgatcacca
    80941 ttctctcgac gctacccacc gcaggggtcg gcgcgctgct ggcattaatg attgccggta
    81001 gcgaactgga tgtgattgcg attatcggca ttattttgct gatcggtatt gtgaagaaga
    81061 acgccatcat gatgatcgac ttcgcgctgg ctgctgagcg cgagcaaggc atgtcgccgc
    81121 gcgatgcaat ctaccaggct tgtctgttgc gttttcgtcc gatcctgatg accaccctgg
    81181 cggctctgct tggcgcgctg ccgctgatgt tgagtaccgg ggtcggcgcg gaactgcgtc
    81241 gtccgttagg tatcggcatg gtcggcggtc tgattgtcag ccaggtgctg acgctgttca
    81301 ccacgccggt gatttatctg ctgttcgacc gcctggcatt gtggaccaaa agtcgctttg
    81361 ctcgccatga agaggaggcg taagtgaagt tttttgccct cttcatttac cgcccggtgg
    81421 cgacgatttt actgtcggtc gccattaccc tgtgcggcat actgggcttc cgtatgctgc
    81481 cggtcgcccc gctgccgcag gtcgattttc cggtgattat ggtcagcgcc tcgctgcccg
    81541 gtgcgtcacc agaaacaatg gcgtcttccg ttgccacgcc gctggagcgc tcgcttgggc
    81601 gcattgccgg ggtcagcgaa atgacctcca gcagttcgct cggcagcaca cgtattattt
    81661 tacagtttga ttttgaccgg gacatcaacg gcgcagcgcg tgatgtgcag gcggcgatca
    81721 acgctgcaca aagtttgctg cccagtggga tgcccagccg tccgacctat cgcaaagcga
    81781 acccgtcgga tgcgccaatt atgatcctca cgctgacatc cgatacttat tcgcagggtg
    81841 aactgtacga tttcgcctcg acgcagctgg ctccgacgat ttcgcaaatc gacggtgttg
    81901 gtgatgtcga tgtcggaggc agctcactgc ccgccgtacg cgtcgggctg aatccgcagg
    81961 cgctgtttaa tcagggcgtg tcgctggacg acgtacgcac cgccatcagc aatgccaacg
    82021 tgcgtaaacc gcagggcgcg ctggaagatg gcactcaccg ctggcagatc cagaccaatg
    82081 atgagctaaa aaccgccgct gaatatcagc cgttgattat tcactacaac aacggcggcg
    82141 cggttcgtct gggcgatgtg gcgacggtga ccgactcagt gcaggatgtg cgcaacgccg
    82201 ggatgaccaa cgccaaaccg gctattttac tgatgatccg caaactgccg gaagccaata
    82261 ttatccagac ggttgacagc atccgggcaa aattaccgga gttgcaggaa accattccgg
    82321 cggcgattga tctgcaaatt gcccaggatc gctcccccac cattcgcgcc tcgctggaag
    82381 aagtcgagca aacgctgatt atctcggtgg cgctggtgat tctggtggtg tttttattcc
    82441 tgcgctcggg tcgcgccact attattcccg ccgtttcggt gccggtttcg ctgattggta
    82501 cgtttgcggc gatgtacctg tgcggattca gtctcaataa cctttcgtta atggcgctca
    82561 ccatcgctac tggtttcgtg gtggatgacg ccatcgtggt gctggaaaac attgcacgtc
    82621 atctggaagc gggaatgaaa ccgttgcaag ccgcactgca aggtactcgc gaagtcggtt
    82681 ttacggtgct gtcgatgagt ctgtcactgg tggcggtgtt cctgccgctg ctgttgatgg
    82741 gcggattgcc gggccgactg ttacgcgagt ttgccgtgac gctttctgtc gccattggta
    82801 tttcgttgct ggtttctctg acattaacgc caatgatgtg tggctggatg ctgaaagcca
    82861 gcaagccgcg cgagcaaaag cgactgcgtg gttttggtcg catgttggta gccctgcaac
    82921 aaggctacgg caagtcactg aaatgggtgc tcaatcatac ccgtctggtg ggcgtggtgc
    82981 tgcttggcac cattgcgctg aatatctggc tgtatatctc gatcccgaaa accttcttcc
    83041 cggagcagga cactggcgtg ttgatgggcg ggattcaggc ggatcagagt atttcgtttc
    83101 aggcgatgcg cggtaagttg caggatttca tgaaaattat ccgtgacgat ccggcagtgg
    83161 ataatgtcac cggctttaca ggcggttcgc gagtgaacag cgggatgatg tttatcaccc
    83221 tcaagccacg cgacgaacgc agcgaaacgg cgcagcaaat tatcgaccgt ctgcgcgtaa
    83281 aactggcgaa agaaccgggg gcgaatctgt tcctgatggc ggtacaggat attcgcgttg
    83341 gtgggcgtca gtcgaacgcc agctaccagt acacgttgtt atccgacgac ctggcggcac
    83401 tgcgcgaatg ggagccgaaa atccgcaaaa aactggcgac gttgccggaa ctggcggacg
    83461 tgaactccga tcagcaggat aacggcgcgg agatgaatct ggtttacgac cgcgacacca
    83521 tggcacggct gggaatcgac gtgcaggccg ccaacagcct gttaaataac gccttcggtc
    83581 agcggcaaat ctcgaccatt taccagccga tgaaccagta taaagtggtg atggaagtgg
    83641 atccgcgcta tacccaggac atcagtgcgc tggaaaaaat gttcgttatc aataacgaag
    83701 gcaaagcgat cccgctgtcg tatttcgcta aatggcaacc ggcgaacgct ccgctgtcgg
    83761 tgaatcatca gggattatcg gcggcctcga ccatctcgtt caacctgccg accggaaaat
    83821 cgctctcgga cgccagtgcg gcgatcgatc gcgcaatgac ccagcttggt gtgccttcga
    83881 cggtgcgcgg cagttttgcc ggaacagcgc aggtgttcca ggagacgatg aactcacagg
    83941 tgatcctgat tatcgccgcc atcgccacgg tgtatatcgt gctgggtatc ctttacgaga
    84001 gttacgtaca cccactgacg attctctcca ccctgccctc ggcgggcgtt ggtgcgctgc
    84061 tggcgctgga gctgttcaat gccccgttca gcctaatagc cctgataggg atcatgctat
    84121 taatcggcat cgtaaagaaa aacgccatta tgatggtcga ttttgcgctc gaagcccaac
    84181 ggcacggtaa cctgacgccg caggaagcta ttttccaggc ctgtctgctg cgttttcgcc
    84241 cgattatgat gactaccctg gcggcgctgt ttggcgcgct gccgctggtg ttgtcgggcg
    84301 gtgacggctc ggagctgcgg cacctcctgg ggatcaccat tgtcggtgga ctggtaatga
    84361 gccagcttct gacgctgtat accacgccgg tggtgtatct ctttttcgac cgtctgcggc
    84421 tgcgtttttc gcgtaaacct aaacaaacgg taaccgagta aatgacagaa cttcccgaca
    84481 gcacccgctg gcaattgtgg attgtggctt tcggcttctt tatgcagtcg ctggacacca
    84541 ccatcgttaa caccgccctt ccctcaatgg cgcaaagcct cggggaaagt ccgttgcata
    84601 tgcacatggt cattgtctct tatgtgctga ccgtggcggt gatgctgccc gccagcggct
    84661 ggctggcgga caaagtcggc gtgcgcaata ttttctttac cgccatcgtg ctgtttactc
    84721 tcggttcact gttttgcgcg ctttccggca cgctgaacga actgttgctg gcacgcgcgt
    84781 tacagggcgt tggcggtgcg atgatggtgc cggtcggcag gttgacggtg atgaaaatcg
    84841 taccgcgcga gcaatatatg gcggcgatga cctttgtcac gttacccggt caggtcggtc
    84901 cgctgctcgg tccggcgctc ggcggtctgc tggtggagta cgcgtcgtgg cactggatct
    84961 ttttgatcaa cattccggtg gggattatcg gtgcgatcgc cacattgctg ttaatgccga
    85021 actacaccat gcagacgcgg cgctttgatc tttccggctt tttattgctg gcggttggca
    85081 tggctgtatt aaccctggcg ctggacggca gtaaaggtac aggtttatcg ccgctggcga
    85141 ttgctggcct ggtcgcagtt ggcgtggtgg cactggtgct ttatctgctg cacgccagaa
    85201 ataacaaccg cgccctgttc agtctgaaac tgttccgcac tcgtaccttt tcgctgggcc
    85261 tggcggggag ctttgccgga cgtattggca gtggcatgtt gccctttatg acaccggttt
    85321 tcctgcaaat tggcctcggt ttctcgccgt ttcatgccgg actgatgatg atcccgatgg
    85381 tacttggcag catgggaatg aagcgaattg tggttcaggt ggtgaatcgc tttggttatc
    85441 gtcgggtact ggtagcgacc acgctgggtc tgtcgctgat caccctgttg tttatgacta
    85501 ccgctctgtt gggctggtac tacgttttgc cgttcgtcct gtttttacaa gggatggtca
    85561 actcgacccg tttctcctcc atgaacaccc tgacgctgaa agatctcccg gacaatctgg
    85621 cgagcagcgg caacagtctg ctgtcgatga ttatgcaatt gtcgatgagt atcggcgtca
    85681 ccatcgccgg gctgttgctg ggactttttg gttcgcagca tgtcagcgtc gacagcggca
    85741 ccacacaaac cgtctttatg tacacctggc ttagcatggc gtttatcatc gcccttccgg
    85801 cgttcatctt tgccagagtg ccgaacgata cgcatcaaaa tgtagctatt tcgcggcgaa
    85861 aaaggagcgc gcaatgaagt tctggcgacc tggtattacc ggcaaactgt ttctggcgat
    85921 tttcgccacc tgcattgttt tgctgatcag tatgcactgg gcggtgcgta tcagttttga
    85981 gcgtggcttt attgattaca tcaagcatgg taatgaacag cgattacaac tgttaagtga
    86041 tgcgcttggc gagcagtatg cgcagcatgg caactggcgc ttcctgcgca acaatgatcg
    86101 ctttgtcttt cagatcctgc gttcatttga acacgataat tcggaagata aacccggccc
    86161 gggtatgcca ccgcacggct ggcgtaccca gttctgggtg gttgatcaaa acaacaaagt
    86221 gctggttggt ccgcgagcgc cgattccacc tgacggtaca cggcgaccca ttctggtcaa
    86281 cggtgcggaa gttggcgcgg tgatcgcctc ccccgttgag cggttaacgc gcaatactga
    86341 tatcaatttc gataaacaac agcggcaaac cagctggttg attgtcgccc tggcaacgtt
    86401 actcgcggca cttgccactt ttctgctggc gcgcggttta ctggcaccgg taaaacgact
    86461 tgtcgatggc acgcacaaac tggcggcggg cgatttcact acccgcgtaa cgcccaccag
    86521 tgaagatgaa ctgggcaaac tggcgcaaga cttcaaccag cttgccagca cactggagaa
    86581 aaaccagcaa atgcggcgcg attttatggc cgatatttct cacgaactgc gtacgccatt
    86641 agcggtgctg cgcggtgaac tggaagctat tcaggatggc gtgcgcaaat tcacgccgga
    86701 gacggtggcg tctttacagg cggaggtcgg tacactgacc aaactggttg acgatctcca
    86761 tcagttgtcg atgtctgatg aaggcgccct cgcctatcaa aaagcaccgg tagatttgat
    86821 cccactgctg gaagtggcgg gcggcgcatt tcgcgaacga ttcgccagcc gtggcctgaa
    86881 actgcaattt tccctgccag acagtattac cgtatttggc gatcgcgacc gtttaatgca
    86941 gttattcaat aatttacttg aaaacagcct gcgctacact gacagcggcg gcagcctgaa
    87001 aatctctgcc gagcagcacg acaaaacggt gcgcctgacc tttgccgaca gtgcgccagg
    87061 tgtcagtgac gagcagctac aaaaattatt tgaacgtttt tatcgcaccg aaggttcccg
    87121 caaccgcgcc agcggtggtt ccgggctggg gctggcgatt tgcctgaaca ttgttgaagc
    87181 acataatggc cgcattattg ctgcccattc gccttttggc ggggtaagca ttacagtaga
    87241 gttaccgctg gaacgggatt tacagagaga agtatgaccg agttaccaat cgacgaaaac
    87301 acaccgcgta ttttgatcgt ggaagatgaa ccgaagctgg ggcagttgct cattgattat
    87361 ctgcgtgctg cgagctatgc gccgacgctt atcagccacg gcgatcaggt actgccgtat
    87421 gtgcgccaga caccaccgga tctgatcctg ttggatctga tgctccctgg caccgatggc
    87481 ttgacgctgt gccgggaaat tcgtcgtttt tctgacattc ctatcgtgat ggtgacggca
    87541 aaaatcgaag agatcgaccg cctgctcgga ctggagattg gcgcagatga ctacatctgc
    87601 aagccgtaca gcccacggga agtggtagcg cgcgtcaaaa ccattttgcg ccgttgcaaa
    87661 ccgcagcgcg aattgcagca acaggatgcc gaaagcccgt tgattatcga cgaaggtcgt
    87721 tttcaggctt catggcgcgg taaaatgctt gacctgacgc ctgcggaatt tcgtctgttg
    87781 aaaacgctct ctcatgaacc aggaaaagtg ttctcccgcg agcaattgct caatcatctt
    87841 tatgacgact accgcgtagt aaccgaccgc accattgaca gccacattaa aaacctgcgc
    87901 cgcaagctgg aatctctcga cgccgaacag tcatttatcc gcgccgttta cggcgttggt
    87961 taccgctggg aagccgacgc ctgccgcatc gtttagtttt agcgacatga ctttgttagc
    88021 cggagatggc gttcagacca gatccggcaa cattatccca cgcatggtca gcaaactg
//
LOCUS       DAJTZL010000002        79579 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.002, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000002 DAJTZL010000000
VERSION     DAJTZL010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 79579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 79579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..79579
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.002"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            2..1197
                     /gene="bluF"
                     /locus_tag="OO544_000089"
                     /pseudo
     CDS             2..1197
                     /gene="bluF"
                     /locus_tag="OO544_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
     gene            1402..2133
                     /gene="bluR"
                     /locus_tag="OO544_000090"
     CDS             1402..2133
                     /gene="bluR"
                     /locus_tag="OO544_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor BluR"
                     /protein_id="HCR6765799.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRRYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTSQDTEDDWNRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAAASLFEMRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPHPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
     gene            2354..2758
                     /locus_tag="OO544_000091"
     CDS             2354..2758
                     /locus_tag="OO544_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HCR6765800.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHHRIAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNLLKVSTTKNPAKIKEAALLHIYGKSTFREYCSTLAGAGVFR
                     WVTDVNHNKRSYYAIDNTLLYIEDVENNKPLI"
     gene            2811..2921
                     /locus_tag="OO544_000092"
     CDS             2811..2921
                     /locus_tag="OO544_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6765801.1"
                     /translation="MGIKVQRLSLWMINTVLLLLPINNHQANTIKLIFEM"
     gene            3157..3201
                     /locus_tag="OO544_000093"
     CDS             3157..3201
                     /locus_tag="OO544_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6765802.1"
                     /translation="MFSKLAPSSIKAMF"
     gene            3454..3777
                     /gene="iraM"
                     /locus_tag="OO544_000094"
     CDS             3454..3777
                     /gene="iraM"
                     /locus_tag="OO544_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HCR6765803.1"
                     /translation="MKWVVIDTVIQPTCGISFSAIWGDMKKIIWYQSTIFLPPGSIFT
                     PVKLGIILKEKEYPITIYNIAPFNKNLWSLLKSSQECPPGESKITNKCLHNSCIIKIC
                     PYGLK"
     gene            complement(3880..5130)
                     /gene="icd"
                     /locus_tag="OO544_000095"
     CDS             complement(3880..5130)
                     /gene="icd"
                     /locus_tag="OO544_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HCR6765804.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGEAIIENM"
     gene            5302..5955
                     /gene="rluE"
                     /locus_tag="OO544_000096"
     CDS             5302..5955
                     /gene="rluE"
                     /locus_tag="OO544_000096"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309634.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HCR6765805.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDTEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYTLDNLANGEWREATE"
     gene            5965..6426
                     /gene="nudJ"
                     /locus_tag="OO544_000097"
     CDS             5965..6426
                     /gene="nudJ"
                     /locus_tag="OO544_000097"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HCR6765806.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILKASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            6480..7586
                     /gene="mnmA"
                     /locus_tag="OO544_000098"
     CDS             6480..7586
                     /gene="mnmA"
                     /locus_tag="OO544_000098"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HCR6765807.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPGQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            7622..8263
                     /gene="hflD"
                     /locus_tag="OO544_000099"
     CDS             7622..8263
                     /gene="hflD"
                     /locus_tag="OO544_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HCR6765808.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            8267..9637
                     /gene="purB"
                     /locus_tag="OO544_000100"
     CDS             8267..9637
                     /gene="purB"
                     /locus_tag="OO544_000100"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HCR6765809.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            9805..10476
                     /gene="phoP"
                     /locus_tag="OO544_000101"
     CDS             9805..10476
                     /gene="phoP"
                     /locus_tag="OO544_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HCR6765810.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            10476..11936
                     /gene="phoQ"
                     /locus_tag="OO544_000102"
     CDS             10476..11936
                     /gene="phoQ"
                     /locus_tag="OO544_000102"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HCR6765811.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            12012..13133
                     /gene="roxA"
                     /locus_tag="OO544_000103"
                     /gene_synonym="ycfD"
     CDS             12012..13133
                     /gene="roxA"
                     /locus_tag="OO544_000103"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HCR6765812.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(13182..14408)
                     /gene="pepT"
                     /locus_tag="OO544_000104"
     CDS             complement(13182..14408)
                     /gene="pepT"
                     /locus_tag="OO544_000104"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HCR6765813.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRK"
     gene            14658..15794
                     /gene="potA"
                     /locus_tag="OO544_000105"
     CDS             14658..15794
                     /gene="potA"
                     /locus_tag="OO544_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HCR6765814.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMREGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            15778..16635
                     /gene="potB"
                     /locus_tag="OO544_000106"
     CDS             15778..16635
                     /gene="potB"
                     /locus_tag="OO544_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HCR6765815.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            16632..17426
                     /gene="potC"
                     /locus_tag="OO544_000107"
     CDS             16632..17426
                     /gene="potC"
                     /locus_tag="OO544_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HCR6765816.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            17423..18469
                     /gene="potD"
                     /locus_tag="OO544_000108"
     CDS             17423..18469
                     /gene="potD"
                     /locus_tag="OO544_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HCR6765817.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKAIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(18625..19446)
                     /gene="cobB"
                     /locus_tag="OO544_000109"
     CDS             complement(18625..19446)
                     /gene="cobB"
                     /locus_tag="OO544_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HCR6765818.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(19462..20373)
                     /gene="nagK"
                     /locus_tag="OO544_000110"
     CDS             complement(19462..20373)
                     /gene="nagK"
                     /locus_tag="OO544_000110"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HCR6765819.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQIGCIENYLSGRGFAWLWQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVCLGNILTIVDSDLVVIGGGLSNFPAITTQLADRLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(20402..21646)
                     /gene="lolE"
                     /locus_tag="OO544_000111"
     CDS             complement(20402..21646)
                     /gene="lolE"
                     /locus_tag="OO544_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HCR6765820.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGTNLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VIIGVVVSLQLTPIIERIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(21646..22347)
                     /gene="lolD"
                     /locus_tag="OO544_000112"
     CDS             complement(21646..22347)
                     /gene="lolD"
                     /locus_tag="OO544_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HCR6765821.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(22340..23539)
                     /gene="lolC"
                     /locus_tag="OO544_000113"
     CDS             complement(22340..23539)
                     /gene="lolC"
                     /locus_tag="OO544_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HCR6765822.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGNFAANSEVDGYEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            23801..24874
                     /locus_tag="OO544_000114"
     CDS             23801..24874
                     /locus_tag="OO544_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HCR6765823.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            25002..28448
                     /gene="mfd"
                     /locus_tag="OO544_000115"
     CDS             25002..28448
                     /gene="mfd"
                     /locus_tag="OO544_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HCR6765824.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            28592..29548
                     /gene="ldtC"
                     /locus_tag="OO544_000116"
                     /pseudo
     CDS             28592..29548
                     /gene="ldtC"
                     /locus_tag="OO544_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707028.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
     gene            complement(29631..29888)
                     /gene="bhsA"
                     /locus_tag="OO544_000117"
     CDS             complement(29631..29888)
                     /gene="bhsA"
                     /locus_tag="OO544_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HCR6765825.1"
                     /translation="MKNVKTLIAAAILSSMSFANFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            30129..30761
                     /gene="comR"
                     /locus_tag="OO544_000118"
     CDS             30129..30761
                     /gene="comR"
                     /locus_tag="OO544_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HCR6765826.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(30823..31362)
                     /locus_tag="OO544_000119"
     CDS             complement(30823..31362)
                     /locus_tag="OO544_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HCR6765827.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(31589..32893)
                     /gene="ndh"
                     /locus_tag="OO544_000120"
     CDS             complement(31589..32893)
                     /gene="ndh"
                     /locus_tag="OO544_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HCR6765828.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELYNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMKNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(33301..33843)
                     /locus_tag="OO544_000121"
     CDS             complement(33301..33843)
                     /locus_tag="OO544_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR6765829.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(33866..34891)
                     /gene="nagZ"
                     /locus_tag="OO544_000122"
     CDS             complement(33866..34891)
                     /gene="nagZ"
                     /locus_tag="OO544_000122"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HCR6765830.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSSYWLKTVLRQELCFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKAGH"
     gene            complement(34902..35726)
                     /gene="thiK"
                     /locus_tag="OO544_000123"
     CDS             complement(34902..35726)
                     /gene="thiK"
                     /locus_tag="OO544_000123"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HCR6765831.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQIGDQQFIRLADDTWRQLLIKQ"
     gene            complement(35707..36348)
                     /gene="lpoB"
                     /locus_tag="OO544_000124"
     CDS             complement(35707..36348)
                     /gene="lpoB"
                     /locus_tag="OO544_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HCR6765832.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(36362..36739)
                     /locus_tag="OO544_000125"
     CDS             complement(36362..36739)
                     /locus_tag="OO544_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HCR6765833.1"
                     /translation="MRKGCFGLVSLALLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVSIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(36742..37101)
                     /gene="hinT"
                     /locus_tag="OO544_000126"
     CDS             complement(36742..37101)
                     /gene="hinT"
                     /locus_tag="OO544_000126"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HCR6765834.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            37435..39624
                     /gene="fhuE"
                     /locus_tag="OO544_000127"
                     /pseudo
     CDS             37435..39624
                     /gene="fhuE"
                     /locus_tag="OO544_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
     gene            complement(39684..41117)
                     /gene="ptsG"
                     /locus_tag="OO544_000128"
     CDS             complement(39684..41117)
                     /gene="ptsG"
                     /locus_tag="OO544_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HCR6765835.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(41412..42209)
                     /locus_tag="OO544_000129"
     CDS             complement(41412..42209)
                     /locus_tag="OO544_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR6765836.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(42220..43224)
                     /gene="holB"
                     /locus_tag="OO544_000130"
     CDS             complement(42220..43224)
                     /gene="holB"
                     /locus_tag="OO544_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HCR6765837.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVVE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(43221..43862)
                     /locus_tag="OO544_000131"
     CDS             complement(43221..43862)
                     /locus_tag="OO544_000131"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HCR6765838.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(43852..44874)
                     /gene="yceG"
                     /locus_tag="OO544_000132"
     CDS             complement(43852..44874)
                     /gene="yceG"
                     /locus_tag="OO544_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HCR6765839.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(44877..45686)
                     /gene="pabC"
                     /locus_tag="OO544_000133"
     CDS             complement(44877..45686)
                     /gene="pabC"
                     /locus_tag="OO544_000133"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HCR6765840.1"
                     /translation="MFLINGHKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMIFCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTVYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(45806..47047)
                     /gene="fabF"
                     /locus_tag="OO544_000134"
     CDS             complement(45806..47047)
                     /gene="fabF"
                     /locus_tag="OO544_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HCR6765841.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLDITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(47135..47371)
                     /gene="acpP"
                     /locus_tag="OO544_000135"
     CDS             complement(47135..47371)
                     /gene="acpP"
                     /locus_tag="OO544_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR6765842.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(47582..48316)
                     /gene="fabG"
                     /locus_tag="OO544_000136"
     CDS             complement(47582..48316)
                     /gene="fabG"
                     /locus_tag="OO544_000136"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HCR6765843.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(48329..49258)
                     /gene="fabD"
                     /locus_tag="OO544_000137"
     CDS             complement(48329..49258)
                     /gene="fabD"
                     /locus_tag="OO544_000137"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HCR6765844.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKLMQEAVPEGTGAMAAIIGLDDASIAKACEEAAAGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVSVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(49274..50227)
                     /gene="fabH"
                     /locus_tag="OO544_000138"
     CDS             complement(49274..50227)
                     /gene="fabH"
                     /locus_tag="OO544_000138"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HCR6765845.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(50295..51365)
                     /gene="plsX"
                     /locus_tag="OO544_000139"
     CDS             complement(50295..51365)
                     /gene="plsX"
                     /locus_tag="OO544_000139"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HCR6765846.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRVALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(51446..51619)
                     /gene="rpmF"
                     /locus_tag="OO544_000140"
     CDS             complement(51446..51619)
                     /gene="rpmF"
                     /locus_tag="OO544_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HCR6765847.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(51671..52192)
                     /gene="yceD"
                     /locus_tag="OO544_000141"
     CDS             complement(51671..52192)
                     /gene="yceD"
                     /locus_tag="OO544_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HCR6765848.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            52391..52975
                     /gene="yceF"
                     /locus_tag="OO544_000142"
     CDS             52391..52975
                     /gene="yceF"
                     /locus_tag="OO544_000142"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HCR6765849.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(53087..54046)
                     /gene="rluC"
                     /locus_tag="OO544_000143"
     CDS             complement(53087..54046)
                     /gene="rluC"
                     /locus_tag="OO544_000143"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HCR6765850.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLIAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            54619..57804
                     /gene="rne"
                     /locus_tag="OO544_000144"
     CDS             54619..57804
                     /gene="rne"
                     /locus_tag="OO544_000144"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HCR6765851.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAATVVAPAPKAATATPAAPA
                     QPGLLSRFFGALKALFSGGEEAKPTEQPTPKAEAKPERQQDRRKPRQSNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTGVPVAAAVEPVVSAPVVEEVAEVVEAPVQIAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEVTVEHNHATVPMTRAPAPEYVPDAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(57999..58952)
                     /gene="flgL"
                     /locus_tag="OO544_000145"
     CDS             complement(57999..58952)
                     /gene="flgL"
                     /locus_tag="OO544_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HCR6765852.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEANGDYVGGTESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKETAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(58964..60607)
                     /gene="flgK"
                     /locus_tag="OO544_000146"
     CDS             complement(58964..60607)
                     /gene="flgK"
                     /locus_tag="OO544_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HCR6765853.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITVANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQELDQTRNTLGQLALAFAEAFNTQHKAGFDANGDAGEDFFTIGKPA
                     VLQNTKNKGDVAIGATVTDASVVLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASEKDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSTTQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(60673..61614)
                     /gene="flgJ"
                     /locus_tag="OO544_000147"
     CDS             complement(60673..61614)
                     /gene="flgJ"
                     /locus_tag="OO544_000147"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HCR6765854.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKAGEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQALSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(61614..62711)
                     /gene="flgI"
                     /locus_tag="OO544_000148"
     CDS             complement(61614..62711)
                     /gene="flgI"
                     /locus_tag="OO544_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HCR6765855.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTESLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(62723..63421)
                     /gene="flgH"
                     /locus_tag="OO544_000149"
     CDS             complement(62723..63421)
                     /gene="flgH"
                     /locus_tag="OO544_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HCR6765856.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(63474..64246)
                     /gene="flgG"
                     /locus_tag="OO544_000150"
                     /pseudo
     CDS             complement(63474..64246)
                     /gene="flgG"
                     /locus_tag="OO544_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
     gene            complement(64418..65173)
                     /gene="flgF"
                     /locus_tag="OO544_000151"
     CDS             complement(64418..65173)
                     /gene="flgF"
                     /locus_tag="OO544_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HCR6765857.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKMDYTSRPLDVALQQDGWLAVQTADGSEGYTRN
                     GSIQVDPTGQLTIQGHQVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAETQATRGPVLQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(65193..66398)
                     /gene="flgE"
                     /locus_tag="OO544_000152"
     CDS             complement(65193..66398)
                     /gene="flgE"
                     /locus_tag="OO544_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HCR6765858.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     TGTAEPAMKLVFNANGVLTSNPTENITTGAINGADPATFSLSFLNSMQQNTGANNIVA
                     TTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWSA
                     TQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQDQ
                     ILNTLVNLR"
     gene            complement(66423..67118)
                     /gene="flgD"
                     /locus_tag="OO544_000153"
     CDS             complement(66423..67118)
                     /gene="flgD"
                     /locus_tag="OO544_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HCR6765859.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVLRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(67130..67534)
                     /gene="flgC"
                     /locus_tag="OO544_000154"
     CDS             complement(67130..67534)
                     /gene="flgC"
                     /locus_tag="OO544_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HCR6765860.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            complement(67538..67954)
                     /gene="flgB"
                     /locus_tag="OO544_000155"
     CDS             complement(67538..67954)
                     /gene="flgB"
                     /locus_tag="OO544_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HCR6765861.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            68109..68768
                     /gene="flgA"
                     /locus_tag="OO544_000156"
     CDS             68109..68768
                     /gene="flgA"
                     /locus_tag="OO544_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HCR6765862.1"
                     /translation="MLAIKRSVAIISILFTPLSAASNLTSQLQTFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRQDTLPPRTVQDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVTQNARVRMVSGLVVSGVVDADGNILIN
                     Q"
     gene            68844..69137
                     /gene="flgM"
                     /locus_tag="OO544_000157"
     CDS             68844..69137
                     /gene="flgM"
                     /locus_tag="OO544_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-28 factor FlgM"
                     /protein_id="HCR6765863.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDASVTNTRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALLNEAQQDLQSN"
     gene            69142..69558
                     /gene="flgN"
                     /locus_tag="OO544_000158"
     CDS             69142..69558
                     /gene="flgN"
                     /locus_tag="OO544_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HCR6765864.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRRKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGGKKISI"
     gene            complement(69598..71133)
                     /gene="murJ"
                     /locus_tag="OO544_000159"
     CDS             complement(69598..71133)
                     /gene="murJ"
                     /locus_tag="OO544_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HCR6765865.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(71243..72166)
                     /locus_tag="OO544_000160"
     CDS             complement(71243..72166)
                     /locus_tag="OO544_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR6765866.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWMLQGAWSPTRAKALP
                     ICESWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSADPLQITTCMHRRAG
                     SQRETVQAVTDDALIDITDMREWREERGQGVVHKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVLQTAGEQAVLAQRIVDKIWRDAMSE"
     gene            complement(72168..72815)
                     /locus_tag="OO544_000161"
     CDS             complement(72168..72815)
                     /locus_tag="OO544_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HCR6765867.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTDMSNAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(72826..73410)
                     /gene="rimJ"
                     /locus_tag="OO544_000162"
     CDS             complement(72826..73410)
                     /gene="rimJ"
                     /locus_tag="OO544_000162"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HCR6765868.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            73646..74854
                     /gene="mdtH"
                     /locus_tag="OO544_000163"
     CDS             73646..74854
                     /gene="mdtH"
                     /locus_tag="OO544_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HCR6765869.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRCRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSALLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     ATRRLLERDA"
     gene            74918..75565
                     /gene="grxB"
                     /locus_tag="OO544_000164"
     CDS             74918..75565
                     /gene="grxB"
                     /locus_tag="OO544_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HCR6765870.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            75699..76259
                     /locus_tag="OO544_000165"
     CDS             75699..76259
                     /locus_tag="OO544_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR6765871.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLISQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            76365..77411
                     /gene="pyrC"
                     /locus_tag="OO544_000166"
     CDS             76365..77411
                     /gene="pyrC"
                     /locus_tag="OO544_000166"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HCR6765872.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            77485..77730
                     /gene="dinI"
                     /locus_tag="OO544_000167"
     CDS             77485..77730
                     /gene="dinI"
                     /locus_tag="OO544_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HCR6765873.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            78020..78274
                     /gene="bssS"
                     /locus_tag="OO544_000168"
     CDS             78020..78274
                     /gene="bssS"
                     /locus_tag="OO544_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415578.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HCR6765874.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFQVNEYRRH"
     gene            78389..79507
                     /gene="solA"
                     /locus_tag="OO544_000169"
     CDS             78389..79507
                     /gene="solA"
                     /locus_tag="OO544_000169"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HCR6765875.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVVSDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFRLSRFQ"
BASE COUNT        19598 a        20912 c        19710 g        19359 t
ORIGIN      
        1 aatgcttacc acccttattt atcgtagcca tatacgtgac gacgaacctg tcaaaaaaat
       61 cgaagaaatg gtttcgatag caaatcgcag gaacatgcag tctgacgtaa cagggatctt
      121 actgtttaat ggttctcatt ttttccagct tctggaaggt ccggaagaac aggttaaaat
      181 gatatatcgg gctatatgcc aggatccacg gcactataat attgttgagc tgctgtgcga
      241 ttacgcgcct gctcgccgtt ttggcaaagc gggaatggaa ttatttgatt tgcgcctgca
      301 cgagcgagat gacgttttac aggccgtatt cgacaaaggc acatcaaaat ttcagctaac
      361 ttatgatgac agagcgctac aattttttcg tacttttgtc cttgcaaccg aacaatcaac
      421 ctatttcgag atccctgccg aagactcctg gctttttatc gctgacggat ctgataaaga
      481 acttgattcc tgtacccttt caccaactat aaacgaccac tttgcctttc atcccttatc
      541 gcggcggata atcgcttttg aagccattgt gcaaaaaaat gaagcccatc agccatagcg
      601 gttgggcagc gtaaagacgg ggaaatctac acagcggatc tcaaaagtaa ggcgcttgca
      661 ttcgcgatgg cacacgcact tgagctcggt gataaaatga tttcaatcaa tctattacct
      721 atgaccctgg ttaacgaacc tgacgcagtc tcttttttac ttgatgaaat aaaggccaat
      781 gctctggtgc ctgaacaaat catcgttgaa tttactgaaa gtgaagttat atctcggttt
      841 gatgagtttg cagaagcgat taaatcgctt aaggctgtcg gtatcagtgt agcaattgat
      901 cattttggcg caggttttgc cggtttgtta ctcctgtcac gcttccagcc tgacagaatt
      961 aaaatcagtc aggaattgat taccaatgtt cataaaagcg ggccacggca ggcaattatt
     1021 caagcgatca taaaatgctg tacatcactt gaaattcaag tcagtgctat gggcgtggca
     1081 acaccagaag agtggatgtg gcttgaatcc gcaggaattg agatgtttca gggagatctg
     1141 tttgctaaag ctaaattgaa tggtatccct tccatagcgt ggccagagaa aaaataattt
     1201 tcagcacatt ctttcacatg atttcagtaa atcatcgtga aaatgaatac gttatgttta
     1261 aatttaatcc acattaaata taatgtttca tccaatttag ctgaaatctt atttatactt
     1321 tgtacaattt tagcgtatat tgtacaaagt attattgggt cgtgtacagg cggcggagat
     1381 ttatgaccgc aaggaggaat tgtggcttat tacagcattg gtgatgttgc tgaacgttgc
     1441 gggattaatc ctgtcactct ccgggcctgg caacgccgct acggtttgtt aaaaccacag
     1501 cgcagtgaag gcggacaccg actctttgat gaagaagaca tacaacgcat cgaagagatc
     1561 aagcgttgga taagtaatgg cgtccctgta ggcaaagtta aagcattact ggaaaccacc
     1621 agccaggata cggaagatga ctggaaccgc ctgcaagaag agatgatgtc aattcttcgt
     1681 atggctaatc ctgccaaact acgcgcgaga attatttcac tgggtcgaga gtacccagta
     1741 gatcaattga ttaatcatgt ttaccttcct gttcgccagc gtctcgtgct tgatcacaac
     1801 acctcccgca ttatgagcag tatgtttgac ggcgcattaa ttgaatatgc agcagcctca
     1861 cttttcgaaa tgcgccgtaa gcccggtaaa gaagccattc tgatggcgtg gaatgttgaa
     1921 gagagagcac gattgtggct ggaagcatgg cgtttatcat tgtcaggatg gcacatttct
     1981 gttcttgctg atcccattga atcgccgcac ccggaactgt tcccgacgca aacattgatt
     2041 gtctggacag gcatggcacc aacgagaagg caaaacgaac ttttacaaca ttggggtgag
     2101 caaggttata aagtcatctt tcatgccccc taatttcacc tttatcccgc cccttagaca
     2161 gaatgttata atctgaaccc acaacagcat ttgtaccgga gctggtccgt tgcaaaatgc
     2221 tcaccggaag aattcaggta atccctctat cacactctgt acatatcaca gccaaaggac
     2281 agaccagatt gaagtggtaa aaccgatcgt gcacactacg cagtctcaca actaacaaga
     2341 ggttattatc ctgatggatc aggtcgttgt ttttcaaaag atgtttgagc aagtgagaaa
     2401 agagcaaaac ttctcctggt tttattcaga attaaaacat caccgtattg cacattacat
     2461 ttattatctg gctacggata acatcagaat tattactcac gatgacacgg ttttgttatt
     2521 aagaggaacc aggaacctgt taaaagttag tacgaccaag aaccctgcta aaataaaaga
     2581 ggccgcattg cttcatattt acggaaaatc tacatttcgg gaatactgtt caacactggc
     2641 aggcgcgggc gttttccgtt gggttactga tgttaatcat aacaaacgca gttactacgc
     2701 cattgataat acgcttttat atattgaaga tgtagaaaat aataaaccat taatctagct
     2761 aaagttggat gcttaagaaa tgcttcataa ttcagtaagg cattagcata atgggaataa
     2821 aagtacagag actatcccta tggatgataa atactgtcct tttattgcta cccataaata
     2881 atcaccaggc caatactatc aaattgatat ttgaaatgtg atcacttgac tttctatacg
     2941 ttattttata acggttaata tatttataaa aacaacgggt gtgccatacg cccgtttcaa
     3001 tacttaacgc acatgtgttt tggtttagtc atcatccagt tatatgtatt ttagccagga
     3061 acaggttaaa cctttcctat ataactcaaa aattgaaacc ttattctcat gtcatgttta
     3121 tattcattat tatcgttata taaaaaggca accataatgt ttagcaaatt ggcaccaagt
     3181 agcataaagg ctatgtttta attacaggat gttcagtcat ttgaatgtat aacattatag
     3241 ctaaacaaat ctaaaacgaa gtgaataatt tattgttttc actaaatctc attttgttta
     3301 acatccattg agattccttg ctttaaattt tgttttatat aagcaatcgt tttaattaat
     3361 ttattttttg aaggggtaat atactcatat gcaaaataaa gaaatgaaca tccaaatgaa
     3421 ctatattaaa taccgtggga taagacataa caaatgaagt gggtagtaat tgacacggta
     3481 attcaaccta catgcggcat atctttttca gccatatggg gtgatatgaa aaagatcatc
     3541 tggtatcaat ctactatatt tcttcctcct ggaagtatat ttacaccggt taagcttggt
     3601 attatcctta aggaaaaaga atatcctatt actatttata acattgcacc attcaacaag
     3661 aatttatgga gtttactcaa aagcagtcaa gagtgtcctc caggagaaag caaaataaca
     3721 aataaatgtt tacataatag ttgcattata aaaatatgcc catatgggct caagtaatgg
     3781 gcttcatata aatatgctcg atatgctatt aataatatcc acataaataa aacaacgggc
     3841 gtgttatacg cccgtttcaa tatttaactc atggagagat tacatgtttt cgatgatcgc
     3901 ttcaccaaac tctgaacatt tcagcagctt agcgccttcc atcagacgtt caaagtcata
     3961 agttacggtt ttcgcgttga tagcaccttc cataccttta acaattaaat cagccgcttc
     4021 agtccacccc atatggcgca gcatcatctc agcggagagg atgatagagc ctggatttac
     4081 tttgtcctga ccggcatatt tcggcgcagt accgtgggtg gcttcaaaca gggcgcattc
     4141 gtcaccgatg tttgcgccag gggcgatacc gataccgcca acctgcgctg ccagggcgtc
     4201 agaaatgtag tcaccgttca ggttcataca ggcgataaca tcatattcag ccggacgcag
     4261 caggatctgt tgcaggaatg catcagcaat cacgtcttta atgacgatct ctttgccggt
     4321 gttcgggttt ttaactttca gccacgggcc gccgtcgatc agttcaccgc caaactcttc
     4381 acgcgccagc tggtagcccc agtctttaaa cgcgccttcg gtgaacttca tgatgttgcc
     4441 tttgtgcacc agagtcacag agtcacgatc gttagcaatt gcgtattcga tcgctgcacg
     4501 aaccagacgt ttggtgcctt cttccgaaca cggcttaata ccgataccac aatgttccgg
     4561 gaagcgaatt ttcttcaccc ccatctcttc acgcaggaat ttaatcactt tctcggcgtc
     4621 ggcagagtct gctttccatt cgatacccgc ataaatgtct tccgagtttt cacggaagat
     4681 aaccatatcg gtcagttcag ggtgtttaac cgggcttgga gtgccctgat agtaacgtac
     4741 cggacgcagg cagatgtaga gatccagttc ctggcgcagg gcaacgttca gagagcgaat
     4801 accgccacca acaggagtgg tcagcggacc tttaatggca acgcgatatt cacgaatcag
     4861 atcaagggtt tcagcaggca gccagacatc ctgaccataa acctgtgtgg atttttcacc
     4921 ggtgtaaatt tccatccagg agattttacg ctcgccttta taggctttct cgactgcagc
     4981 gtcgaccact ttcagcatgg ctggggttac atctacaccg attccatcac cttcaatgta
     5041 agggataatc ggattttcag gaacgttgag tttgccgttt tgcagggtga tcttcttgcc
     5101 ttgtgccgga acaactactt tactttccat tcacctctcc ttcgagcgct actggtttgc
     5161 tcgtctgcca ccacgttgca tatgcgtttg cgtcctgcga tacggatgct ttagagcaat
     5221 tttttgttaa tgatttgtaa ttggcttgtc aatgctaccc tattgtttgc cgtcatgaaa
     5281 gatgcgcgtt attaggctat aatgcggcaa ttcataatct ctgaaaatac catgcaaaaa
     5341 acttctttta gaaatcacca ggttaagcga ttcagctcgc aacgttctac taggcgtaaa
     5401 cctgaaaacc agcccacgcg tgtgatcctg ttcaataaac cctacgatgt tcttccgcag
     5461 ttcaccgatg aagccggacg caaaacatta aaagaattca tcccggttca gggcgtttat
     5521 gcagcaggtc gtcttgaccg cgataccgaa gggttactgg tgctgaccaa taacggcgcg
     5581 ttgcaggcgc gtttaaccca gccgggcaaa cgcaccggga aaatctatta tgtgcaggtg
     5641 gaaggtattc ccacacaaga cgcacttgaa gccttgcgca atggcgtaac cttaaatgat
     5701 ggccctaccc tgcccgccgg cgcggagctg gttgacgaac cagcgtggtt atggccgcgg
     5761 aatccaccaa ttcgtgaacg caaaagtatt cccaccagct ggctgaagat caccttatat
     5821 gaaggacgta atcgccaggt gcgccgcatg accgcccatg ttggtttccc cacgctgcga
     5881 ctgattcgtt atgcgatggg tgattacact ttggataacc ttgccaatgg cgaatggcga
     5941 gaagcaacag aataaggatg cacaatgttt aaaccgcacg ttaccgttgc ttgcgtagtg
     6001 cacgcagagg gtaaattttt agtcgttgaa gagacgatta atggtaaagc gttatggaac
     6061 caacctgccg ggcatctgga agccgatgaa accttagtgg aagctgccgc ccgtgagcta
     6121 tgggaagaaa ccggcatcag cgcgcaaccg caacacttta ttcgtatgca tcagtggatt
     6181 gcgccagata aaacgccgtt tttgcgtttc ctctttgcca ttgagcttga gcaaatatgc
     6241 ccgacccagc ctcatgacag cgatatcgac tgctgtcgtt gggtcagcgc cgaagaaatt
     6301 ttaaaggcgt caaatcttcg ttcgccgctg gtggcggaaa gtatccgttg ttatcaaagt
     6361 gggcaacgct atccgctgga gatgattggc gattttaact ggccttttac aaagggtgtc
     6421 atctaaaagg ctgcgtgata gaatacgccg ccttgaagtt caatgtcgtg agtgatccaa
     6481 tgtctgaaac cgcaaaaaaa gtaatcgtcg gcatgtccgg cggtgtcgat tcctccgttt
     6541 ctgcctggct gttgcaacaa cagggatatc aggttgaagg cctgtttatg aagaactggg
     6601 aagaagacga cggtgaggaa tattgcacag cggcagcgga tctggctgat gcccaggctg
     6661 tctgcgacaa gctcggcatt gaactgcaca ccgttaactt tgctgccgag tactgggaca
     6721 atgtcttcga actgttcctt gccgaatata aagccggtcg cacgccgaat ccggatattc
     6781 tgtgcaacaa agagatcaaa tttaaagctt tcctcgaatt tgccgccgaa gatttaggtg
     6841 ccgattatat cgctaccggt cattacgtac gtcgggccga tgtcgatggc aagagccgcc
     6901 tgctgcgtgg tctggacagc aataaagacc agagctactt cctttatacg ctcagccatg
     6961 agcagattgc gcaaagcctg ttcccggtcg gcgaactgga aaaaccgcag gtgcgtaaga
     7021 ttgctgaaga tcttggtctg gtcaccgcga agaaaaaaga ctctaccggc atttgcttca
     7081 ttggcgaacg taaattccgc gagttcctgg gccgttatct cccggggcaa ccgggcaaaa
     7141 tcattaccgt cgatggcgat gaaattggtg agcaccaggg gctgatgtat cacactctcg
     7201 gtcagcgtaa aggtctgggt atcggtggca ccaaagaagg caccgaagaa ccttggtatg
     7261 tggtggacaa agacgtcgaa aacaacattc tggttgtcgc tcagggccat gaacacccgc
     7321 ggctgatgtc tgtcgggttg attgcccagc agttgcactg ggtcgatcgc gaaccattca
     7381 ccggcactat gcgttgcacg gtaaaaaccc gctatcgcca gaccgatatc ccttgcaccg
     7441 tcaaggcgct ggacgatgat cgcattgaag tgattttcga tgaaccggtt gccgccgtga
     7501 cgccgggcca gtctgccgtc ttctataacg gtgaagtgtg cctcggtggc ggtattattg
     7561 agcagcgtct gccgctgccg gtctgattat tatctttact taaaacaagg aagcagtgaa
     7621 cgtggcaaag aattactatg acatcaccct cgccctggcc ggtatttgtc agtcggcacg
     7681 cctggtgcaa caactcgctc accaggggca ttgtgatgcc gatgcgctac acgtctcact
     7741 caacagtatt attgatatga accccagctc gacgctggcg gtttttggcg gtagcgaagc
     7801 caacctgcgc gtcgggctgg aaaccctgct cggcgtgctc aatgccagca gtcgccaggg
     7861 cttaaacgcc gaattaaccc gctacacact cagcttgatg gtgcttgagc gcaaactctc
     7921 ctcagcgaaa ggcgcgctcg acactctggg caaccggatc aacggcctgc aacgccagct
     7981 cgaacacttc gatttacagt ccgaaacgct gatgagcgcg atggctgcta tctatgttga
     8041 tgtgatcagc ccgcttggcc cacgcattca ggtcaccggt tcccctgctg tactgcaaag
     8101 cccacaagtg caggcgaaag ttcgcgcaac cctgctggca ggcattcgcg ccgccgtgct
     8161 ctggcaccag gtcggcggcg gacgtctgca actgatgttt tctcgtaatc gcctgaccac
     8221 tcaggcaaaa caaattcttg ctcatttaac cccggagttg tgatctatgg aattatcctc
     8281 actgaccgcc gtttcccctg tcgatggacg ctacggcgat aaagtcagcg cgctgcgcgg
     8341 gattttcagc gaatatggtt tgctgaaatt ccgtgtacaa gttgaagtac gttggctgca
     8401 aaaactggcc gcgcacgcag cgatcaagga agttcctgct tttgctgccg acgcaatcgg
     8461 ttaccttgat gcaattgtcg ccaatttcag cgaagaagat gccgcacgca tcaaaaccat
     8521 cgagcgtacc actaaccacg acgttaaagc ggttgagtat ttcctgaaag aaaaagtggc
     8581 ggagatcccg gaactgcacg cggtttctga attcatccac tttgcctgta cttcggaaga
     8641 tatcaataac ctctcccacg cattaatgct gaaaaccgcg cgtgatgaag tgatcctgcc
     8701 gtactggcgt caactgattg atggcattaa agatctcgcc gttcagtacc gcgatatccc
     8761 gctgctgtct cgtacccacg gtcagccagc cacgccgtca accatcggta aagagatggc
     8821 aaacgtcgcc taccgtatgg agcgccagta ccgccagctt aaccaggtgg agatcctcgg
     8881 caaaatcaac ggcgcggtcg gtaactataa cgcccacatc gccgcttacc cggaagttga
     8941 ctggcatcag ttcagcgaag agttcgtcac ctcgctgggt attcaatgga acccgtacac
     9001 tacccagatt gaaccgcacg actacatcgc cgagctgttt gattgcgttg cacgcttcaa
     9061 caccattctg atcgactttg atcgtgacgt ctggggttat atcgccctta accacttcaa
     9121 acagaaaacc attgctggtg agattggttc ttccaccatg ccgcataaag ttaacccgat
     9181 cgacttcgag aactccgaag ggaatctggg cctttccaac gcggtattac agcatctggc
     9241 aagcaaactg ccggtttccc gctggcagcg tgacctgacc gactccaccg tgctgcgtaa
     9301 cctcggcgtg ggtatcggtt atgcgctgat tgcgtatcaa tccaccctga aaggcgtgag
     9361 caaactggaa gtgaaccgtg accatctgct ggatgagctg gatcacaact gggaagtgct
     9421 ggctgagcca atccagacag ttatgcgtcg ctatggcatc gaaaaaccgt acgagaagct
     9481 gaaagagctg actcgtggta agcgcgttga cgccgaaggc atgaagcagt ttatcgacgg
     9541 tctggcgttg ccagaagaag agaaagcccg cctgaaagcg atgacgccgg caaactacat
     9601 tggtcgcgcc atcaccatgg ttgatgagct gaaataaact cgtatcagtg ccggatggcg
     9661 atgctgtccg gcctgcttat taagattatc cgctttttat tttttcactt tacctcccct
     9721 ccccgctggt ttatttaatg tttaccccca taaccacata atcgcgttac actattttaa
     9781 taattaagac agggagaaat aaaaatgcgc gtactggttg ttgaagacaa tgcgttgtta
     9841 cgtcaccacc ttaaagttca gattcaggat gctggtcatc aggtcgatga tgcagaagat
     9901 gccaaagaag ccgattatta tctcaatgaa catttaccgg atattgcgat tgtcgatctc
     9961 ggattgccag acgaggacgg tctgtcactg attcgccgct ggcgtagcaa tgatgtttca
    10021 ctgccgattc tggtattaac cgcccgtgaa agctggcagg acaaagtcga agtattaagt
    10081 gctggtgctg atgattacgt gactaaacca tttcatattg aagaggtgat ggcgcgaatg
    10141 caggcattaa tgcggcgtaa tagcggtctg gcttcacagg tcatttcgct accaccgttt
    10201 caggttgatc tctctcgccg tgaattatct attaatgacg aagtgatcaa actgaccgcg
    10261 ttcgaataca ccattatgga aacgttgata cgcaataatg gcaaagtggt gagcaaagat
    10321 tcgttaatgc tccaactcta tccggatgcg gagctgcggg aaagccatac cattgatgta
    10381 ctgatgggac gtctgcgcaa aaaaattcag gcacaatatc cgcaagaagt gattactacc
    10441 gttcgcggcc agggctattt gttcgaattg cgctgatgaa aaaattactg cgtctttttt
    10501 tcccgctctc gctgcgggta cgttttctgt tggcaacggc tgcggtagta ctggtgcttt
    10561 cgcttgccta cggaatggtc gcgctgatcg gttatagcgt cagtttcgat aaaactacgt
    10621 ttcggctgtt acgtggcgag agcaatctgt tctataccct tgcgaagtgg gaaaacaata
    10681 agttgcatgt cgagttaccc gaaaatatcg acaagcaaag ccccaccatg acgctaattt
    10741 atgatgagaa cgggcagctt ttatgggcgc aacgtgacgt gccctggctg atgaagatga
    10801 tccagcctga ctggctgaaa tcgaatggtt ttcatgaaat tgaagcggat gttaacgata
    10861 ccagcctctt gctgagtgga gatcattcga tacagcaaca gttgcaggaa gtgcgggaag
    10921 atgatgacga cgcggagatg acccactcgg tggcagtaaa cgtctacccg gcaacatcgc
    10981 ggatgccaaa attaaccatt gtggtggtgg ataccattcc ggtggagcta aaaagttcct
    11041 atatggtctg gagctggttt atctatgtgc tctcagccaa tctgctgtta gtgatcccgc
    11101 tgctgtgggt cgccgcctgg tggagtttac gccccatcga agccctggca aaagaagtcc
    11161 gcgaactgga agaacataac cgcgaattgc tcaatccagc cacaacgcga gaactgacca
    11221 gtctggtacg aaacctgaac cgattgttaa aaagtgaacg cgaacgttac gacaaatacc
    11281 gtacgacgct caccgacctg acccatagtc tgaaaacgcc actggcggtg ctgcaaagta
    11341 cgctgcgttc tctgcgtagt gaaaagatga gcgtcagtga tgctgagccg gtaatgctgg
    11401 agcaaatcag ccgcatttca cagcaaattg gctactacct gcatcgtgcc agtatgcgcg
    11461 gcgggacatt gctcagccgc gagctgcatc cggtcgcccc actgctggac aatctcacct
    11521 cagcgctgaa caaagtgtat caacgcaaag gggtcaatat ctctctcgat atttcgccag
    11581 agatcagctt tgtcggtgag cagaacgatt ttgtcgaggt gatgggcaac gtgctggata
    11641 atgcctgtaa atattgcctc gagtttgtcg aaatttctgc aaggcaaacc gacgagcatc
    11701 tctatattgt ggtcgaggat gatggccccg gtattccatt aagcaagcga gaggtcattt
    11761 tcgaccgtgg tcaacgggtt gatactttac gccctgggca aggtgtaggg ctggcggtag
    11821 cccgcgaaat caccgagcaa tatgagggta aaatcgtcgc cggagagagc atgctgggcg
    11881 gtgcgcggat ggaggtgatt tttggtcgcc agcattctgc gccgaaagat gaataaatat
    11941 gtccatactt cacgcattac gttaagcatc cgttataatc ggttgcagat accagcctgt
    12001 ggatgcttaa catggaatac caactcactc ttaactggcc cgattttctt gaacgtcact
    12061 ggcagaaacg cccggtggtg ttaaaacgcg gctttaataa ttttattgac ccgatctctc
    12121 cagacgagtt ggcgggtctg gcgatggaaa gcgaagttga cagtcgactg gtcagtcacc
    12181 aggatggcaa atggcaggtc agccacggcc cgttcgaaag ctacgatcat ctcggtgaaa
    12241 ccaactggtc attactggta caggcagtga accactggca tgagccgacc gccgcgctga
    12301 tgcgaccgtt ccgtgaacta ccggactggc gtattgatga tctgatgatt tctttttctg
    12361 tacctggcgg cggcgtcggc ccgcatctcg atcagtacga cgtgtttatc attcagggta
    12421 ccggacgtcg tcgctggcga gtgggcgaaa agctgcaaat gaaacagcac tgcccacatc
    12481 cggatctgtt acaggtcgat ccgttcgaag ccatcatcga tgaagagctg gagcctggtg
    12541 atattcttta tattccgcca ggattcccgc atgaaggcta cgcgctggaa aatgcgatga
    12601 actattccgt gggctttcgc gcgccaaata cgcgggaact gattagtgga tttgccgatt
    12661 atgtgctgca acgtgaactg ggcggcaact actacagcga tcctgatgtt ccacctcgcg
    12721 ctcatcctgc ggatgttctg cctcaagaga tggataaact gcgtgagatg atgctcgaat
    12781 tgatcaacca gccggaacac tttaagcaat ggtttggcga gtttatatcc cagtcacgtc
    12841 atgaactgga tatcgcgccg ccagaaccgc cttatcagcc agatgaaatc tacgatgcgc
    12901 tgaaacaagg tgatgtgctg gtgcgcctgg gtggtctgcg cgtattgcgc attggcgacg
    12961 acgtgtatgc caatggtgag aagatcgatt ccccgcaccg tccggcactg gatgcactcg
    13021 ccagcaacat tgcgctgact gcggagaatt ttggcgatgc gctggaagat ccgtcattcc
    13081 tcgcgatgct cgcggcgctg gtcaatagcg ggtattggtt cttcgaaggg taagtttgga
    13141 tttaagccgg atgcggcatc acatgccgca tcccttttcg cttacttccg ttgcgccgtt
    13201 aactcggcaa tacggacgat cacctgcacc gctttttcca taccttccag agtcacaaac
    13261 tcatgcttac catgatagtt gtaaccgcca gtgaacaagt tcgggcacgg taatcccata
    13321 aacgacaact gcgcgccgtc ggtaccaccg cggatcggtt tcagttccgg ttcaatatcg
    13381 cagtcacgca tcgcctgctg ggcgatatcg agaatatgcg gatgctcaac cactttctct
    13441 cgcatattgt agtaactgtc ttcaatcacc agttcaatgt agcaatcagg atgtaaccct
    13501 ttgcccactt ttttggcgat ctccatcatt ttacgtttac gcgcttcaaa ctgtttacgg
    13561 tcgaaatcac ggatgatgta gtgcatatcg gcccgttcaa cggtgccttt catgctcgcc
    13621 aggtgataga aaccttcata gccttctgtc atttccgggc tttcatccgc cggaacttcc
    13681 gcatgaatac gtgccgccag cgacagcgca tttaccatca ctcctttcgc cgtgcccgga
    13741 tgaacattgt taccgacaat tttgatattg accgatgcgg cgttgaagtt ttcaaactcc
    13801 agttcgccta cgccaccacc gtcaacagtg taagcccaac gggcatcgaa ggcatcaaca
    13861 tcaaaatgtt tcgccccttt gcccacttct tcatccgggg taaaggcgac gcgaatatca
    13921 ccatgcggaa tgtttttctg ttgcaatacc gccagcgcgg tcatgatttc tgcaatacct
    13981 gctttgtcat cggcacctaa caaggtttta ccatcggtgg taatcaacgt ctgacccagt
    14041 agctgatgca gcaccgggaa cataaccggt gataaaactt catcgccgat acccagcgca
    14101 atatcgccac cgcgatagtt ttcaacaatt tgcggattaa catttttgcc gctgcaatcc
    14161 ggtgaggtat ccacatgaga aataaagcca atcgccggga tatcgccagg gacgttagcc
    14221 ggtaacgtcg ccatcaaagt gcccttctca cttaaggtca cattgataag ccccatttct
    14281 tcgagctgct ctttcagcag atgcaataac ttccattggc cttccgtgct gggaacctgt
    14341 ctcacccctg cttttgattg ggtatccaga gatacgtagt tcaaaaatcg ctcaagtagt
    14401 ttatccatgt agtcaccctc actttttgtg acaacattat taagaagcaa gaaaagacaa
    14461 atattgcgtc aggtcacttt tacccctgca agcgggaata tttatcagca ttaccttaat
    14521 gaatataaag ctaagccagt aattcacaac aaagattggc gattcaagcg gtttgccgta
    14581 gaattaccgc cctcattaag agtcaccaag gtggttaacc acaaaccccg catcggtaag
    14641 ccatccgttg cgtttacatg ggacagagta aaaaattgaa taaacaaccg agttcgcttt
    14701 caccgctggt gcaattggcg ggaattcgca aatgctttga tggtaaagag gtcattcccc
    14761 agctggatct gactatcaac aatggcgagt tcctcacgct gcttggccct tctggctgcg
    14821 gtaaaacaac cgttcttcgc ctgattgcag gtctggaaac tgttgattcc ggacgcatca
    14881 tgctggataa cgaggacatc acccacgttc cggcggaaaa ccgctatgtg aacactgttt
    14941 tccaaagcta cgcacttttc ccccacatga ccgtgttcga aaatgtggcc tttgggttgc
    15001 gcatgcaaaa aacccccgct gctgaaatta cgccccgcgt gatggaagcc ctgcggatgg
    15061 tgcaactgga aaccttcgct caacgtaaac cgcatcagct ctctggtggt caacagcaac
    15121 gcgtcgccat tgctcgcgcg gtggttaaca agcctcgtct gttgttgctg gatgagtcgc
    15181 tctcagcgct ggattacaaa ctgcgtaagc aaatgcagaa cgagctgaaa gcgttacagc
    15241 gtaagcttgg tattaccttc gtctttgtca ctcacgatca ggaagaagcg ctcaccatgt
    15301 cagacaggat tgtggtgatg cgcgagggtc gtattgagca agatggtaca ccgcgcgaaa
    15361 tttacgaaga gccgaaaaac ctgtttgttg ccggcttcat tggcgaaatc aatatgttta
    15421 acgccactgt catcgaacgt ctcgacgagc agcgcgtacg cgccaacgtt gaaggccgcg
    15481 aatgtaatat ctacgttaac ttcgccgttg aaccggggca aaaactgcat gttctgctgc
    15541 gcccggaaga cttacgtgtt gaagagatta acgacgacaa ccacgctgaa gggctgattg
    15601 gttacgttcg tgagcgtaac tacaaaggca tgacgctgga gtcggttgtt gaactggaaa
    15661 atggcaagat ggtgatggtc agcgaattct tcaatgaaga cgaccctgac tttgaccact
    15721 ctctcgacca aaaaatggcc attaattggg tagaaagctg ggaggtcgta ctggctgatg
    15781 aagaacacaa gtaagttcca gaatgtagtg attgtcacta ttgtcggttg gcttgtgttg
    15841 tttgtctttc tgcccaacct gatgatcatt ggcaccagct ttttgacccg cgacgacgcc
    15901 agtttcgtca aaatggtctt tacgctggat aactacacgc gtctgctcga tccgctctat
    15961 tttgaagtgc tattgcactc gctgaatatg gcgctgatcg ccaccctcgc ctgcctggtg
    16021 ctgggctacc cgtttgcctg gtttctggcg aagctgccac acaaggtgcg tccgctgctg
    16081 ctgtttctgc tgattgttcc cttctggacc aactcactga ttcgcatcta cgggctgaaa
    16141 attttcctca gcaccaaagg ctatctcaac gagtttttgc tctggctggg cgttatcgac
    16201 acaccaattc gtatcatgtt cacgcccagt gcggtgatta tcggtctggt ttacattctg
    16261 ctaccgttta tggtgatgcc gctgtactcc agtatcgaaa aactggataa gccgttgctt
    16321 gaggcggcgc gcgatctcgg tgccagcaag ttacagactt ttatccgtat cattattcca
    16381 ctgacgatgc cgggaattat tgccggatgt ctgctggtga tgctgccagc gatgggcctg
    16441 ttctatgtat ccgacctgat gggcggtgcg aaaaacctgc tgatcggtaa cgtcatcaag
    16501 gtccagttcc tcaatattcg tgactggccg tttggtgcag ccaccagcat tacgctgact
    16561 atcgtaatgg gcctgatgtt gctggtttac tggcgcgctt ctcgtctgct gaataaaaag
    16621 gtggaactcg aatgatcggt cgactgcttc gcggcggttt tatgaccgct atctacgcgt
    16681 acctgtatat cccaatcatt attttgattg tgaactcctt taacagctcg cgctttggca
    16741 tcaactggca gggttttacc accaaatggt atagcctgct gatgaacaac gacagcctgt
    16801 tacaggcagc gcagcattca ctaacaatgg cggtgttttc ggcgacattt gctacgctta
    16861 tcggttcact gacggcagtt gcgctgtacc gttatcgctt tcgtggtaag ccgttcgtta
    16921 gcggaatgct gtttgtggtg atgatgtcac cagatatcgt gatggcgatt tctctgctgg
    16981 tgctgtttat gctgctgggt attcagcttg gcttctggtc gctgctgttc tcgcatatca
    17041 ccttctgcct gccgtttgtg gtggtgacgg tgtattcgcg cctgaaaggt tttgacgtgc
    17101 ggatgctgga agcggcgaaa gatctcggtg ccagtgaatt taccattctg cgaaaaatca
    17161 ttctgccact ggcaatgcca gcggtggcgg cgggctgggt gttaagcttt accctgtcga
    17221 tggacgatgt ggtagtttct tcgttcgtca ccggaccaag ttatgaaatt ctgccgttaa
    17281 aaatttattc gatggtcaaa gtcggcgtat cgccggaagt taacgcgctg gcaaccatat
    17341 tactggtgct gtcgctggtg atggttattg ccagccagct tattgctcgt gataaaacga
    17401 aaggtaacac aggggacgtt aaatgaaaaa atggtcacgc cacctgctcg cggcgggtgc
    17461 tctggcactg ggcatgagcg ccgctcacgc cgatgacaac aacacgctgt atttctacaa
    17521 ctggaccgag tacgtgccgc caggactgct tgaacagttc accaaagaaa ccggtattaa
    17581 ggctatctat tcgacttacg agtcgaacga aaccatgtac gccaagctga aaacgtacaa
    17641 agacggtgcc tatgacctgg tggttccttc aacctattac gtcgataaaa tgcgtaaaga
    17701 agggatgatc cagaagatcg acaagtcgaa gttaacgaac ttcagcaatc tcgatccaga
    17761 catgctcaac aagccgtttg acccgaataa cgactactcc attccgtata tctggggtgc
    17821 gacggcgatt ggcgttaacg gtgatgcggt ggatcctaaa tctgtcacca gttgggcaga
    17881 tctgtggaag ccagagtaca aaggtagcct gctgctgacc gacgatgccc gtgaagtgtt
    17941 ccagatggcg ctgcgcaaac tgggctactc cggtaacacc accgatccga aagagattga
    18001 agctgcatat aacgagctga aaaaactgat gccaaacgtc gcagcgttta actccgataa
    18061 cccggctaac ccgtacatgg aaggcgaagt taacctcggc atgatctgga acggctctgc
    18121 tttcgttgca cgccaggcgg gtacgccaat tgacgtggtg tggccgaaag aaggcggcat
    18181 tttctggatg gacagcctgg cgatcccggc aaatgccaaa aacaaagaag gcgcgctgaa
    18241 attgatcaac ttcctgctgc gcccggatgt ggcaaaacag gttgccgaaa ctatcggtta
    18301 tccaacgcca aaccttgcgg cgcgtaagct gttaagcccg gaagtggcga acgataaaac
    18361 gctctacccg gatgctgaaa ccattaaaaa tggtgaatgg cagaatgacg ttggcgcagc
    18421 cagcagcatt tatgaagagt attatcagaa gctgaaagca ggacgttaat tcagcaaacg
    18481 ggcggtggta ttaccgcccg ttttttagtt agcaaggatg tggctaatga gtcaggacag
    18541 taaagtttta tcccgctctt tcgtcggtat tgtggtcccc ttgtaggcct gataagcgta
    18601 gcgcatcagg caatgcttcc tgctttataa tcccttcagc aacttttcaa caaactcagg
    18661 caccacctgg cttgccgggc cgtaatattt ctcggcaaat tcattaccga cctgacttgg
    18721 ttcgagattc agttccacgg tgtgcgcgcc atgcagtttc gcttcgtgaa caaacccagc
    18781 cgccggataa acatgcccgg aagtaccaat ggcaatgaaa atatcagcca tcgacaacgc
    18841 catataaatt tcatccatgc cgagtggcat ttcgccaaac caaacgacgt gcgggcgcaa
    18901 gggtgccggg aactggcaac aatggcattt atcttctggc gtaacgtctc cggtccagtc
    18961 gagaacctga ccactttgcg aacaacgaac tttcagcaat tccccatgca tatgaatgac
    19021 attggtatta cctgcgcgtt catgcaggtt gtctatattc tgcgttacca gcaaaaagcg
    19081 atcgccgagg gcgtcttgca gtttagccag cgcaagatgc gcggcgttag gctgaatttc
    19141 tggctgctgc agctgtcgac gacgggcgtt ataaaacgct tgcaccagtt caggatcgcg
    19201 atcgaaacct tccggcgttg ccacatcttc aacccgatgt tcttcccaca ggccatctgc
    19261 ggcgcgaaag gtacgaatac ctgattccgc agaaattcct gcccctgtca gtacgagtac
    19321 tcttggtttt tccattgctt ccggcaccac tttatctctg aaaaaaatac gctgacgcaa
    19381 acgctcgcgc aggcggcgtt tatttttacg aaaacgactt aaccgatgac cccgacgcga
    19441 cagcatagca acctctgttg tttaatcggt tagatgtagg aaggccgcac cacgcattcc
    19501 tcccgcatca ccgtggcgcg cgcgttcaat gcgcggaaca cgagctacag gtaagagatg
    19561 acgaggcagc ctgtccgcca gttgcgttgt gattgccggg aaattcgata agccaccacc
    19621 aatgacgacc aggtcagagt caacgatggt caggatattt cccagacaaa ccgctaataa
    19681 atccagataa cgctcaacgt gcgcccttgc ctgctcatcg ccttgatcat aaagcgcaat
    19741 aatttcggga gcctgcaacg gttgatgata atagtgttgc cagagccacg caaagccccg
    19801 acctgataga taattttcga tacaaccaat ttgaccacag ccgcagcggc gtaacgggaa
    19861 atccagcccc atcatggtta acgcatcaac cggcagacgc atatggccaa actcgccggt
    19921 aatgtagctt ttaccggtaa tcggtttacc gttgaaaatc agcccgccgc caacgccggt
    19981 gccgagaatt aaccccatca ccagtggata ttgcgtaaat tcatcatccc aggcttctga
    20041 aagggcaaaa cagttggcat cgttatcaag gcgtacatcg cgatcaagac gtgcgctcag
    20101 gtcggcacgc agcggtttac cgctggcagc ggggacattg gcggcataca gcgtaccatc
    20161 ttctgtttcc ggcattcccg gaataccgat gccaacagaa cctttacagc caaaacgttg
    20221 gtcagcttcg gctaccagct cgcatactgc atctaaaaat gcgtcatagc tgtcacgcgg
    20281 tgtcggcacc cgcttttccc actgcaactg ccgaccgcta tcaaacacgc caagcgcaat
    20341 ttttgttcca ccaatatcaa acccgtaata cattgccgct ccttgtttta atgtactgcc
    20401 tttactggcc gctaaggact cgcgcagggt caatattact ggcgcgccgc gccggatacc
    20461 aacttgccaa aagactcagc aacaatgctg tgaccagtac gtagaagacg tccagccagt
    20521 gcaattccga tggcaggaag tcaataaaat agatatcgct ggagaggaac tgatgaccga
    20581 tcagcttttc aatccgctca ataatcgggg taagttgcag tgaaacaact acgccgataa
    20641 tcacaccaca caggctaccg aacagccctg ccagcaatcc ataccagaca aagatggcgc
    20701 gaattaaacc atctttcgcc cccagcgttc ttaatactgc gatatcgcca ctcttgtctt
    20761 tcaccgccat cactaaggtg gagacgatgt tgaaacaggc cacgccaatc accagtacca
    20821 tcgccagata cataatggcg cggatcatct ggatatcgcg atacatatag ccgtaagtac
    20881 caatccaact tttaatataa acatagctgt tggtcacttc acccgcatcg cgtaccagct
    20941 tattggcgtt gaaaacatcc gtcattttaa gggcaatacc tgacacgctg gaacccatat
    21001 caagatattg ttgggcatcc gccagcggga tcatggcaaa actgtgatcg agttgaccac
    21061 tcaactgcaa aataccggca atgtgcaaac gcacacgttt tggctgcatc agtttatgct
    21121 cgggattcga gttgggaatc atgatcgaca cccaatcgcc ctgcttcact ttcagcgcat
    21181 ccgccacgcc tttgccgatg ataatttgct gttcgcccgc tttaaaattg cgccaggcat
    21241 ccccctgaac aaacgagggt aatgcgctca gacgctgttc ctgttgcggg ttaacgccct
    21301 tcacctgaat tgcgcgcaga ttggtaccac tttctaccag tccggtgaaa ttgatatacg
    21361 gcgcagcggc ggcaataccc ggcacttttt gcacgttatc cagtgcttcc tgccagttag
    21421 tccatggctg atttaccgcc tcaatttcac cgtgcggtac cacagcaaga atgcggttat
    21481 tcagttcgcg ttcaaagccg ttcatcgcgc ttaagccgac gatcaatacc gccacgccga
    21541 gggcaatgcc aatggtagaa atgacagaga ttagcgacac catgccgccg cgtcgccgtc
    21601 cgcgactaaa acgcaggcca atcaataacg ataaaggcat cgccattact ccgcccccat
    21661 caggctcagt tccgccgtca gacgtccatc acgcatttcc agttggcggc tcatacgttt
    21721 cgccagttgc aggtcgtgag taaccaccag gaaggcggtg ccctgcaagc gattcaattc
    21781 cccaagcaac tggaagatgc tgtcggcgtt acgcgcatcg aggttaccgg taggctcatc
    21841 cgcaagtacc agacgcggat tattgaccag cgcacgggca atcgccacac gctggcgttc
    21901 gccgccagaa agttcagatg ggcggtgatt cgcacgatgc tccagcccca ccgcttttaa
    21961 catctccagc gcacggctgt tgatttcagc gggctttttc ttgccaatca gcagtggcat
    22021 agccacgttt tccagggcag taaaatccgg cagcaggtgg tgaaactgat aaataaagcc
    22081 cagcttctgg ttgcgcagtt ctgctttcgc cgccgaagac agtttgctca tcggttgacc
    22141 gttaaagatc acatcgccgg aggttggtgt atccagcccg cccagcaggt gcagcaaggt
    22201 acttttaccg gaaccagagc taccgacgat cgccatcatt tcaccttcgc cgacgctgaa
    22261 actgacattg tgcagtacat cggtttgcac actgccttcc tgatagcgtt tgcacaggtt
    22321 gtcgcattgc aacaggatct tattcataac gtaaagcctc agcgggttga gtggcggcag
    22381 cgcgccatga agggtaaagc gtagacagca gcgcgatagc catcgccacc agcgcaataa
    22441 caatgacctg taaaggttcg atagcgaccg gcagcgccgc gccatcaagc aggacgccga
    22501 ttatcggcat cagattattt aactggctgg caagcagtgc gccaagcgcc gctccgagga
    22561 tcgcaccgat aatcccggcg ctggcccctt gcaccataaa gaccatcatg atttgtcgcg
    22621 gagttaagcc ttgcgtttgc aggatcgcga cttcgccctg cttctccatt accatcagcc
    22681 ctagtgaggt aataatgtta aacgccgcaa cggcgacaat caggctcagc agcaagccca
    22741 tcatattttt ttccatgcgt acggcctgga acagctcgcc tttacggtca cgccagtcct
    22801 gccatttgct gccttcaggc agtttttgct gacttaaaga gtcaactttc agcggctcat
    22861 ccagccacaa acgccagccg gtaatattgc ctgccggata acgcatcaga cgcgaggcat
    22921 cctcaatatt caccagcatt tcatagccat cgacttcact gttggcggcg aaattgccaa
    22981 tcacattgaa caggcgctgg cttggaatac gccccatcgg cgtgaactgg ctggcagatg
    23041 gtaccatcac gcggatttga tcaccgcgat taacgcctag ctgtgaggca agttgttcgc
    23101 cgaggatgac attatatttc cccggctcga ggtcagtttg tttcacattg accagatacg
    23161 gcgtaagtgg atctttttgc gccgggtcga taccgagcat caccccgacc gccacgctgc
    23221 gcgcgctttg cagtaccaca tcaccggtag taataggtgc gacgcgatta acgccgtcca
    23281 gtttgactgc cgtttccggg agttgctgcg ggttaagaga gccatgctca gaagagagaa
    23341 ttgcctgtgg catcaggcca aggatgttgt tttgcagctc gcgctcaaag ccgttcatca
    23401 ctgacaatac tgtgaccagc gccatcaccc cgagggtaat gccgatggta gaaagccagg
    23461 agacgaaacg accgaagcga tccgctgcac gcccacgcat gtaacgcagg ccaataaata
    23521 gagcgacagg ttggtacatg aaatccgtct ggttgctgta gcaaagacac gagtatataa
    23581 gcgaaagcaa gatgggtaaa cgtctgaact gtaagtgatc gtcagggcta atctgaaaag
    23641 cccccgtaaa tcaatcggct aaattgccat gttttatcat caatcttgcc gcctgatctt
    23701 aatctgaaaa atcatccaat acagacagat acccattaca cctcgggtca taacccgcag
    23761 gatcaaccgc atgtaaatta aaaaagagcg tgttgtcctg atgaaacaaa aagagctatg
    23821 gattaaccag atcaaagggt tatgtatttg tctggtggtg atttatcact cggtcattac
    23881 cttttatccg catatgacca cttttcagca tccgttatcg gaagtcctga gtaaatgctg
    23941 gatctatttc aatctttacc ttgcaccctt tcgtatgccg gtttttttct ttatatccgg
    24001 ctatttgatt cgccgctata tcgacagcgt accgtgggga aattgtctcg ataaacggat
    24061 ctggaacatc ttctgggtgc tggcgctttg gggcgtggtg cagtggctgg cgttaagtgc
    24121 gctgaaccag tggctggcac ctgagcgcga tttaagcaat gcctccaatg ccgcttatgc
    24181 cgattccacc ggtgagttcc tgcacgggat gatcaccgcc agcaccagct tgtggtatct
    24241 gtatgcgtta attgtctatt tcgtggtatg taaaattttt agccgtctgg cgctgccact
    24301 attcgccttg tttgtactgc tgagtgtggc ggttaatttc gttcccacgc cgtggtgggg
    24361 aatgaacagt gtgatccgca atttgcctta ttacagcctt ggcgcatggt ttggcgcaac
    24421 aataatgacc tgtgttaaag aggtgccgtt gcgccgccat ctgctgatgg cttctttgct
    24481 taccgttctg gcggtcggtg cctggttgtt tactatctcg ctgctgttgt cgctggtatc
    24541 gattgtggtg atcatgaagc tgttttatca gtacgagcaa cgattcggta tgcgctccac
    24601 cagcctgctg aatgtgattg gctccaacac cattgctatc tacaccaccc atcgcattct
    24661 ggttgaaata ttcagcttaa ctctgcttgc gcaaatgaac gcagcacgct ggtcgccgca
    24721 agtcgaactg acactcctgc tggtttatcc ctttgtcagt ttgttcatct gtactgttgc
    24781 gggcttgctg gtaagaaaac tttcacagcg tgcattcagc gatctgttgt tctccccgcc
    24841 ttcactgccc gctgccgtca gttactctcg ttaaaccatc gccccagccg gggcgattca
    24901 atttgctaaa ccatgtcgat tacggataat gaacagcatt gcttatcaga gatatgcccc
    24961 catatgttga ggcatatcct aacgagaatc tgacaaccgt tatgcctgaa caatatcgtt
    25021 atacactgcc cgtcaaagcg ggtgagcagc gtctgctggg cgagttaacc ggcgcagcct
    25081 gtgcaacgct ggtagcggaa attgccgaac gtcacgccgg tccggtggta ctcattgcac
    25141 cagatatgca aaatgctctg cgtttgcatg atgaaatcag ccagttcacc gatcagatgg
    25201 tgatgaatct ggcggactgg gaaactcttc cctacgacag tttttcgcct catcaggaca
    25261 ttatctcctc gcgcctttcc accctttacc agctaccgac gatgcagcgt ggcgtactga
    25321 ttgttccggt gaatacgctt atgcagcgcg tttgcccaca cagttttctc cacggtcatg
    25381 cgctggtgat gaaaaaaggt cagcgcctgt cacgagatgc attacgaacc caactggaca
    25441 gcgccggtta tcgccatgtt gaccaggtga tggagcacgg cgaatacgcc acgcgcggcg
    25501 cgttgctgga tctcttcccg atggggagtg agctgcctta tcgtcttgat ttctttgatg
    25561 atgaaatcga cagcctgcgg gtgtttgacg tcgacagcca gcgcacgctg gaggaagtag
    25621 aagcgatcaa tctgctgccc gcgcacgaat ttccgaccga taaagcggca attgaactgt
    25681 tccgcagcca gtggcgcgat accttcgaag tgaagcgcga tccggaacat atttaccagc
    25741 aagtgagtaa aggcacatta cctgccggga tcgagtactg gcagccattg ttcttcagcg
    25801 aaccactgcc gccgctgttc agttatttcc ctgccaatac cttgctcgtg aatactggcg
    25861 atctggaaac cagtgccgaa cgtttccagg ctgacacgct ggcgcgtttt gagaatcgcg
    25921 gcgtcgatcc gatgcgcccg ctgttgccac cacaatcgct ctggctgcgg gtggacgagc
    25981 tcttctcaga gctgaaaaac tggccccggg tgcagctaaa aactgaacat ttaccgacaa
    26041 aagccgcgaa tgccaattta ggtttccaga aactgccaga cctggccgtt caggcacaac
    26101 aaaaagcgcc gctggatgcg ctgcgtaagt tcctcgagac tttcgacggt ccggtggtgt
    26161 tctcggtaga aagtgaaggt cgccgtgaag cgctgggtga actgctcgca cgaattaaaa
    26221 ttgctccgca acgcattatg cgtcttgatg aagccagcga ccgtgggcgt tatctgatga
    26281 ttggcgctgc cgaacatggt tttgtcgata aggtgcgtaa tctggcgctg atctgcgaaa
    26341 gcgatctgct cggtgaacgt gttgcccgcc gtcgtcagga ttctcgccgc accatcaacc
    26401 ccgatacact gatccgtaac cttgcggagc tgcatattgg tcagccggtg gtccatctgg
    26461 agcacggtgt cgggcgctat gccggaatga ccacgctcga agcgggcggc attactggcg
    26521 agtatttgat gctcacctat gccaacgacg ccaaactgta tgttccggtg tcgtcactgc
    26581 atctgattag ccgttacgca ggtggcgcgg aagaaaacgc cccgctgcat aaacttggcg
    26641 gcgatgcgtg gtcacgcgcg cggcagaaag cggcggaaaa agtgcgtgat gtggcggcgg
    26701 aattgctgga tatctacgcg caacgcgccg ccaaagaggg cttcgcgttt aaacacgatc
    26761 gtgagcagta tcagttgttc tgcgacagct tcccgtttga aaccacgccg gatcaggcgc
    26821 aggccattaa tgcggtactt agcgacatgt gtcagccgct ggcaatggat cgtctggtgt
    26881 gcggcgatgt tggctttggt aaaacagaag tggcgatgcg cgccgctttc ctggcagtag
    26941 ataaccacaa gcaggtggcg gtgttggtgc ctaccaccct tctcgcgcag cagcattacg
    27001 acaacttccg cgaccgtttc gccaactggc cggtacgtat cgaaatgatc tcccgtttcc
    27061 gcagcgccaa agagcagacg caaatccttg cggaagtggc ggaagggaaa atcgatattc
    27121 tgatcggtac gcacaaactg ctgcaaagtg acgtcaagtt taaagattta ggcctgctga
    27181 ttgtcgatga agaacaccgc ttcggggtgc gtcataaaga gcgcattaaa gcgatgcgcg
    27241 cgaacgtgga tattctgacg cttactgcaa cgccgatccc acgcacgctg aatatggcaa
    27301 tgagcggaat gcgtgacctg tcgattatcg ccacgccgcc cgcccgtcgt ctggcagtta
    27361 aaacctttgt ccgtgagtat gacagcctgg tggtccggga ggcgatcctg cgtgaaattt
    27421 tgcgcggggg gcaggtttat tatctctaca atgatgtgga aaacatccag aaagctgccg
    27481 aacggctggc agaactggtg ccagaagcgc ggatcgccat cggtcacggg cagatgcgcg
    27541 agcgcgaact ggaacgggtg atgaatgatt tccatcatca acgtttcaac gtgctggttt
    27601 gtacaaccat tatcgaaacc gggatcgaca tcccgacagc caacaccatt atcattgaac
    27661 gcgcggatca cttcggtctg gcgcagctgc accagttacg cggtcgcgtc ggacgttcgc
    27721 atcatcaggc atatgcatgg ttgctgacac cgcatccaaa agcgatgact accgatgcac
    27781 aaaaacgtct tgaagcgatt gcctcgctgg aagatctcgg ggcaggtttt gcgctggcaa
    27841 cgcacgatct ggagattcgc ggcgcgggtg aactgcttgg cgaagaacaa agcggctcaa
    27901 tggaaaccat cggtttctcg ctgtatatgg agttgctgga aaacgccgtc gatgcactga
    27961 aagccggacg cgagccgtcg ctggaagatc tcaccagcca gcaaacagaa gtcgagctgc
    28021 ggatgccgtc gctattgcca gatgatttca ttcctgacgt gaatacgcgt ttgtcgttct
    28081 ataaacgtat tgccagcgcc aaaacggaaa acgaactgga agagatcaaa gtcgagctta
    28141 tcgatcgctt cggcctgctg ccagatccgg cgcgtaccct gctggatatt gcccgtctgc
    28201 gccagcaagc gcagaaactg gggatcagga agctggaagg taatgagaaa ggcggggtga
    28261 tcgaatttgc cgagaagaat cacgttaatc cggcctggtt gattggtttg ctgcaaaaac
    28321 agccgcagca ttaccgcctt gatggtccga cgcgcctgaa atttattcag gatttgagtg
    28381 agcggaaaac gcgtatcgaa tgggtacgcc agtttatgcg tgaactggaa gagaacgcga
    28441 tcgcttaatg gtaaatcggg ggcgtttctg cgcccccatc tgcaacattt acaaattctt
    28501 tgcacttccc tgcactatcc gacactgtca ccatccataa ttcaggctta tctgtttatt
    28561 acaataacct tatatttatt attgattttt ggtgatgatc aaaacgcgtt tttctcgctg
    28621 gctaacgttt tttacgttcg ccgctgccgt ggcgctaccg gcaaaagcca acacctagcc
    28681 gctgccgcca gcgagcagtc gtctggttgg cgaaaacaaa tttcatgtgg tggaaaatga
    28741 cggtggttct ctggaagcca tcgccaaaaa atataacgtc ggctttctcg ctctgttaca
    28801 ggctaacccc ggcgttgatc cttacgtacc gcgcgcgggc agcgtgttaa cgatcccgtt
    28861 gcaaacccta cttccagacg cgccgcgcga aggcattgtg atcaacattg cggagctgcg
    28921 tctctattac tacccgccgg gtaaaaattc ggtaaccgtg tatccaatag gtattggtca
    28981 gttaggtggt gacacgctga caccgacaat ggtgaccacc gtttcagaca aacgtgcaaa
    29041 cccaacctgg acgccaacgg caaacatccg cgcccgttat aaagcacagg gaattgagtt
    29101 gcctacggta gtgccggctg gaccggataa cccaatgggc catcatgcga ttcgtctggc
    29161 ggcctatggc ggcgtttatt tgcttcacgg tacgaacgcc gatttcggca ttggcatgcg
    29221 ggtaagttct ggctgtattc gtctgcggga tgacgatatc aaaacactct ttagccaggt
    29281 caccccaggc accaaagtga atatcatcaa cactccgata aaagtctctg ccgaaccaaa
    29341 cggtgcgcgt ctggttgaag tacatcagcc gctgtcagag aagattgatg acgatccgca
    29401 gctgctgcca attacgctga atagcgcaat gcaatcattt aaagatgcag cacaaactga
    29461 cgctgaagtg atgcaacatg tgatggatgt ccgttccggg atgccggtgg atgtccgccg
    29521 tcatcaagtg agcccacaaa ctctgtaaat cctggataaa aaaaatcccc gcgacacggc
    29581 atgtcgcggg gaaaaaatca gcagtagcag gcattaatga gggttaatgc ttatttataa
    29641 attactgctg ttccatggag ggtattcgga ccggttacag aagtaatacg gaaagatttt
    29701 gcgcccatct catccgcttt ttgcgccagc tgctcttcca gcgatcccag atttgtcccc
    29761 gcgttagcac tgatagtacc gactttttgt tggccttctg gcgttgactg aacttcgaca
    29821 gccgcaaagt tggcaaatga catggagctt aaaatcgccg cagcgatgag ggtttttacg
    29881 tttttcataa tagtggcctt atgcagatga atgacgtcgc aagaaattac ttaacgatcg
    29941 ataactaaat gatagatgtg atctggatca catacaagat attttttata acaatcatta
    30001 attaatttaa aaatctttaa atttcagtta aatagaaagc aattattttt atgggatctg
    30061 gactggtgaa cggaggtgat tatttttata ataacctttc attcacagtc accaggttca
    30121 acggcatcat ggcaactgac tcaacacaat gtgtaaaaaa aagccgtggc cgcccaaaag
    30181 tgttcgacag ggatgccgcg cttgataagg ccatgaaatt gttctggcaa cacggttatg
    30241 aagcgacttc tcttgcggac ctcgtcgaag cgaccggagc caaagcgccc acgctatacg
    30301 cggaatttac caacaaagag gggttatttc gtgccgttct cgaccgctat atcgatcgtt
    30361 ttgccgctaa gcatgaagca cagctgtttt gtgaagagaa aagcgtggag tctgcgctgg
    30421 ctgactattt tgctgccatc gccaactgct ttaccagcaa agacaccccg gcaggctgct
    30481 tcatgatcaa caactgcacc accctctccc cagattcagg agatatcgcc aatacgttga
    30541 aatcacgcca tgcgatgcaa gagcgcactt tgcagcagtt tttatgtcaa cgacaagcgc
    30601 gcggggaaat cccgacccac tgtgacgtga cacatctggc agaattcctt aattgtatta
    30661 ttcaggggat gtcgatcagc gcacgcgaag gtgcatcgct ggaaaaactg atgcagattg
    30721 ccggaacgac tttgcgttta tggcccgaac tggtgaaata agtcgcaaaa aaagcccctc
    30781 agcctgagcg aatgaggggc caaattgcag agtacagcct tgttatactt tgttattcaa
    30841 aatcagttgc ccgttactat ctagcgggat ctgcgtaccc ggatcgcggt ccatgcggat
    30901 tttgccctgc tgatcgccaa tcttataggt cacatcataa ccgagcattt tttctgactt
    30961 gtcatacacc gttttacaac gctgttgcgt agtcgtgtaa gtatcgcttt cctggagaga
    31021 gccctggatc tggttaccgg catatccacc acccagcgcc cccacaacag tggcgacatc
    31081 tttaccacga ccaccaccaa actgatgccc tatcacgccg ccagcaacag cgccgagcac
    31141 cgacccggta atgcgatttt catcctgcac cggtcgacga tgggtcactg tgacgttgcg
    31201 acactcctga cgcggtgttt taaccgtttc cttgattggg gttgcagaaa caacctgagc
    31261 gtattgcggg ccccgttcaa acacgttcag actggccact gccgctacgc ccagcgcagc
    31321 tgcgacacca atcccgatac ccgccaacat tgatttattc acgggaacac ctccttcttt
    31381 gcagttatgc caatcatgca agcgaaggag aaaagcagct atcagacaga agatgaaaag
    31441 cgcgggaatg gctggaaata cttaaggatt cggagaagtc tgaaggaatg cctgatgcga
    31501 cgcttaacgc gtcttatcag gcctaccgct cgtaccattt gtccagggcg gatgaggcgt
    31561 ttatgccaca tccgccagtg tacgtcgatt aatgcaactt caaacgcgga cggataacgc
    31621 ggttaatact ccccaccagc atcattaatc cggttttaaa gtaaccatgc agcgcaatct
    31681 gatgcattcg gtatagcgag atatatacaa agcgcgcaat tcgtccttca atcatcattg
    31741 agccgcgcgt caggttaccc atcaggctac cgacagtgga gaagttcgac agcgatacca
    31801 gcgaaccatg atctttatac tgataatttt tcagcggttt accgttcatc tgcgccagaa
    31861 tatttttcat tgcgcaggtc gccatctggt gtgcagcctg agcacgcggc ggaacaaagc
    31921 ccccttccgg acgcgggcat gacgcgcagt cgccaatagc gtaaatgtct ggatcgcggg
    31981 tggtttgcag cgtcggttcc accaccagct ggttgatacg gttggtttca agaccaccga
    32041 tatctttcag gaagtctggc gctttgatcc cggctgccca caccatcaga tcagcctcaa
    32101 tatattcacc atctttagtg tgcaggccgc cttcatcagc actggtgacc atggtttgcg
    32161 tcagcacgcg aacgccaagt ttcgttagct cgttgtgggc cgcagcagag atacgtggcg
    32221 gtaatgcagg caaaatacgt tctcccgctt ctaccagcgt tacgttcagg gcttcgttgg
    32281 tcaggccttt gtaaccgtag ctgtgcagtt gcttgaccgc attgtacaat tcagcggaga
    32341 gttctacacc cgtcgcgccg ccgccgacaa tcgcaatgtt cactttacca ttcgcgccca
    32401 ggttggcgga gtatttcagg aacagattca gcatctcctg gtggaagcga cgcgcctggt
    32461 gcgggttatc gaggaaaatg cagttctctt tgacacctgg cgtattgaaa tcgttagagg
    32521 tgctacccag cgccattacc agggtgtcat aggcgatttt acgttccgga accagcagtt
    32581 cacctttctc atcgcgcagt tctgcaatag tgattgtttt cgcttcacga tcaatatcaa
    32641 tgacggaacc cagctggaac tggaaaccat gattgcgcgc atgggccaga tagctcaacg
    32701 catcgacgcc ttcatcaagc gagccagtcg ccacttcgtg cagcagcggt ttccacagat
    32761 ggctgtggtt acgatcgacc agcgtaattt tggctttttt cttgcgtccc agcttatgcc
    32821 ccagctgtgt tgccatttcc agcccaccag caccgccgcc gacaatcaca atttttttca
    32881 atggcgtagt caacgtgacc cccttaaatt tattaaccaa ttgttaatta atggttataa
    32941 acatagctct taattaacaa caggttacag caatgaaaat gttcttcgct attgagaata
    33001 acatgaatgg tgcattggtc ataccaaaat tgatgttaat caagttttgt tgctgaaaag
    33061 ataggcgact ttaccgggtg agatcaaaaa acagagttta ttgtttatta tttgagcaag
    33121 tccggaaggg tgatataggg tgacgcgtat atcgagggcg tttggtcgtc ctgataagat
    33181 gcgcagcatc gcatcaggca ttgatgctcg actgtcggat gcggcgtaaa cgccttatcc
    33241 gacttacaga attcggagcg catcaggtac cggcaaggtt acctgatgcg acgagggcat
    33301 ttacccgagg gttttgaacg ccttaatgcg ctgcaaatgc ggggagatat tcttgaattt
    33361 gtgcgtctgc tcttcgtccc agacaatctc gtaataatga tgcaactctt cagatgtccg
    33421 ctggctgtta agcgcttcat cattacgcga caaaatcacc aggcaacgat cgcgattctt
    33481 ctcgcggaag ttggtcacac acttagtggc aatatcggca tactcttccg ggcgatcaat
    33541 cttgccttcc atgttctcat aagggaacaa attagggttg aagatcacct ggcggatatc
    33601 gcaaagaaaa ccaatccgtt ccgcccagta tccgcccaga ccaacgccgc aaatcagcgg
    33661 acgctcgtcg acgttcagtt gcaacatttt gtccacttct ttgagcagat gctgcatatc
    33721 atgtttcgga tgccgcgtac tgtagcttat caagcgtaca tccgggtcaa taaactgcaa
    33781 ttgtaagact ttctcgtggt tacccggact gttagagtca aaaccgtgta aatagataat
    33841 catcgcttcc tcacataagc cagggttagt gacctgcttt ctcttcctgc cagcgttcat
    33901 gtaactgatt cagacgggtg ctgatcgcct tccagcgagc cgagtccatc agttcctgtc
    33961 gcgaaaatga acctttatga tacaaacgtg taacacgttc tgccttgatc ggtgacagat
    34021 tatctaacac gctgacggcc cctttacgat tattgcagac caggatcata tcgcaacctg
    34081 cgtccagtga tgcctgcccg cgttcggcat aactgcccat aatcgcggca ccttccatcg
    34141 ataaatcgtc agagaaaatt acaccgtcaa aacacagttc ctgacgcaaa acggttttca
    34201 gccagtagga agaaccgctc gccggacgcg gatcaacatc actgtagatc acatgcgcag
    34261 gcataatggc gtcgagttta ttttcgcgga ttaaggaact gaagaccgac atatctttag
    34321 cgcgaatctc cgcttgtgga cgtggatcgc acggtgtttc tttgtgtgag tctgccgtta
    34381 ctgcaccgtg tcctgggaag tgtttcccgg tcgttttcat tccggcttca tgcataccat
    34441 caataaaccg gctggcaatt gccagggctt tttgtggatc ggcatgataa gaacgctcgc
    34501 caatcgccgc gctgatatgc ccgacatcca gcactggcgc aaagctgata tcaatatcca
    34561 tagcgatcat ttcgctggcc atcagccaac ccgcctcttg cgccagtttg ccgccctctt
    34621 ccattcccaa cagcgcagca aaggattgtg ctgccggtaa gcgggtaaaa ccttcgcgaa
    34681 aacgctgcac gcgtccacct tcctgatcta ccgccaccac cagatgattg cgcgatgctg
    34741 cgcggatctg gcgcaccagt tcacgtaact gggcaggatc atgatagtta cgcgtaaaga
    34801 gaatcagccc tcccaccagc ggatgcgcca gtatttcacg ctcttccgcg tccagttcgt
    34861 aaccttcgac atccaacatt actggaccca cactgctctc cttattgttt tattaatagc
    34921 tgccgccagg tgtcatcggc cagcctgata aattgttgat cgccgatttg tcgccagcgg
    34981 tactcaaacc accctgcttt gagcatcagc agccagggaa accatcgcct gacctgacgc
    35041 cataattgcg ccggataaat cttcgcgcga gtggcatagt cattgaccaa ttgccggtgc
    35101 tgttcagtat tttccaccca caccgccgcc agttccagcg cgatatcacc atctccggca
    35161 tactcccagt cgatgagttt taaccctgac gcgctatgca ctaaatttcc ggcgtggaca
    35221 tccatatgca atggacttaa gcgtaaaggc cgtggttccc gcgctttgcg cagacgtttt
    35281 aacattcgca gccaacccac agtccgccgc gccggatcgc tttgctgcca gtacagttcc
    35341 agtaagggca acagcgttat tcgccagcca aaacgtggtt gttgatgaag ataatacagc
    35401 aagcctgcca gttcgttggt atccggcaaa tacgttttta ccgcgccggg cagatagtcg
    35461 actaccatcc agtcacggag atataaatgc ggcttcggtg caatgcttgc gggtagttgt
    35521 gataaagccc gatactggcg caagaacgcg gactgcggcg catcaggatc gtgcggctga
    35581 cgcacaacaa aacgctggcc ctgatgttca atgagaaaac taccgccact aagcccacta
    35641 ttaaacgtcg tgacgggatg aaactgcggg aaaaagcgcg acagcaattc gtcgcgcgtg
    35701 atgggattat tgctgcgaaa cggcaccttt acctgaccag ataatttcgc ccgtctgcac
    35761 cagcatcagc tgcatttgta gggtcggagc gttaacgttg ccagaggcgc tggagtacag
    35821 cacgtaatga gcgccgacat tgcgggcaat gcctatggct ttactacggg tacctaaact
    35881 gtcctgcggc gacaaaccta actgttgctt agccatcgac agctgctggg cggaaaccag
    35941 ggtaaatttc ccgttattag ccagcgcatt tcgcagcgtt tcggtcgctt ctgcggcatt
    36001 cagcgaaccg ttagtacggt tgttaacgct atcaaccagc aggacgctac ccgcagtcac
    36061 cccgtcagcc ccaagcatct tactgaccat cggctgcatt gcgccattcc agtcataatg
    36121 gcgaatatgc ggcgcaggcg gtgccgtttg atcttcgtgc tcaattgggc ctggctgctg
    36181 cgggatcgtc ggcaccgagg gcactgtggg gacaggctgt tgtggctcgg ctggttgttc
    36241 cggcgctggt ttcacttctt ctaccggtgc aggttcacgt tgccccacac acccggcgag
    36301 aaacatcgcc agcgcggtaa tcaaggcgta gcgactcatt tttgtcatca agattcaccc
    36361 cttacaaata tagataaagt ctgactttgt gcgcccccag aaaattggcg ctgccgtaca
    36421 gcgttaccgc cgaatgtgcg ggaatgctaa cgctgcgtgg cctttccaga ggatgcatct
    36481 ccagccctct ggcgtcatac cagtaaaaac gataatgaac ggtaacgggt tcttgccttt
    36541 cgttataaag cgttgaggat gctgaaggtt gaatatcaga cgtcgaaagg acgggctttt
    36601 ctgcactgat gcccgcagcc aataaagatg actccatcac cagcgattgc tcatcattca
    36661 ccggaatttc cggatgtgaa cgacagccca ccagcagtaa caacgccaga gacaccagcc
    36721 caaagcatcc ttttctcatc gttacagacc tttatgcgcc agcattggtc ccagcggacg
    36781 gccacccaac aagtgcatat ggatgtggta aacctcttgt ccgccatggc ggttggtatt
    36841 catgatcaga cgatagccat cttcggcaat accttcttgc tcagcaattt ttgccgctac
    36901 ggtgatcatg cgtcccagcg cctgctcatg ctcagctgac acgtcgttca cagtcggaat
    36961 gaggatattc ggaatgatca gaatatgcgt tggcgcttgc ggcgaaatat cgcgaaacgc
    37021 cgttaccaga tcatcctggt agacgatatc ggaggggatc tcacgacgaa taattttgct
    37081 gaatatagtt tcttctgcca cgacattttc ctttttcaaa tagccatgcg ttgcgccatg
    37141 ctacgccgcg ccacactgcg agtatagagt atggtcgagt taaacctgct ctttcaacct
    37201 taactcacga tttcttaagc aaaaaatcgt tttgctgccg tatatatcgc cattattccc
    37261 atttctgcct gcgaatgcgc tgaaaagaca ttctctgttt ggtttacacg acaaatatga
    37321 atgcgcatat ttctcatttg catttacaaa caaaattatt cgcacactaa atataaccgc
    37381 tcatcaccct cctccggcat gagcctgacg acaacataaa ccaagagatt tcaaatgctt
    37441 tcaacacaat ttaacaggga taatcaatat caagccatca ccaaaccgtc actacttgcc
    37501 ggttgcatag cactggcact attaccttct gccgcttttg ctgcaccagc cactgaagaa
    37561 acggtgattg ttgagggttc agccacagct ccagatgatg gcgaaaatga ttacagcgta
    37621 acgtctacct ctgcgggtac caaaatgcag atgactcaac ttgatattcc tcagtcggtc
    37681 actattgtta gccagcagcg gatggaagat cagcagttac aaacgctggg cgaagtgatg
    37741 gaaaacacgc tggggatcag caaaagtcag gcggattccg atcgtgctct ttattattcc
    37801 cgtggattcc agatcgataa ctatatggtt gatggtatcc ccacctattt tgaatcgcgc
    37861 tggaatctgg gcgacgcact ttctgatatg gcactctttg aacgcgtaga agtagtgcgt
    37921 ggcgcgacag gactcatgac cgggacgggt aatccatctg cggcaattaa tatggttcga
    37981 aaacacgcga ccagtcgtga atttaaaggc gatgtctcgg cggaatacgg tagctggaac
    38041 aaagaacggt atgtggcgga tttacaaagc ccactcaccg aagacggtaa aatccgcgcg
    38101 cgaattgtcg gcggctacca gaataacgac tcatggctgg accgctacaa cagtgaaaag
    38161 accttcttct cgggcattgt cgatgctgat ttaggcgatc ttacgacgct ttcagccggt
    38221 tacgaatatc agcgcattga tgttaatagc ccaacctggg gcggtttacc gcgctggaat
    38281 actgatggca gcagcaacag ttacgatcgt gcacgcagta ccgcacctga ctgggcgtac
    38341 aacgataaag agatcaacaa ggtctttatg accctgaagc agcggtttgc tgatacctag
    38401 caagcgacac tgaatgccac ccactctgaa gtcgaatttg acagcaaaat gatgtatgtc
    38461 gatgcctatg taaacaaagc ggatggtatg ctggttgggc catacagtaa ttatggacct
    38521 ggctttgatt atgtcggcgg caccggttgg aacagtggca aacgtaaagt tgatgcgctg
    38581 gatttgttcg ctgacggtag ttatgaattg tttggtcgtc agcacaatct aatgtttggt
    38641 ggcagttaca gcaaacaaaa caatcgttac ttcagttcat gggccaacat cttcccggat
    38701 gaaattggca gtttctacaa ctttaatggc aatttcccac aaaccgactg gtcaccacag
    38761 agcctggcac aggacgatac cacacatatg aaatcgttat atgctgctac tcgagtcacc
    38821 cttgccgatc cgctgcatct gatcctcggc gcacgttata ccaactggcg ggttgatacg
    38881 ctgacttaca gcatggagaa aaaccacacc acgccttacg ctggtctggt gtttgacatc
    38941 aatgacaact ggtcgaccta cgccagctat acctctattt tccagccgca aaatgatcgt
    39001 gacagttcag gcaaatatct ggctccaatc accggtaaca actacgagct gggtctgaaa
    39061 tcggactgga tgaatagccg tctgaccacc acgttagcca tcttccgtat tgagcaggat
    39121 aatgtcgctc agtccaccgg tacacctatc cccggcagca acggcgaaac cgcctataaa
    39181 gcggtggatg ggacagtcag taaaggggtg gaatttgaac tcaacggcgc aattaccgac
    39241 aactggcagc tgacatttgg cgcaacgcgc tatattgcag aggataacga aggaaacgcc
    39301 gttaatccta atctgccacg caccacggtt aaaatgttca ccagctatcg gttgcctgtc
    39361 atgccagagt tgacagtcgg cggtggtgtt aactggcaaa atcgcgtgta taccgacacc
    39421 gtgacaccgt atggcacctt ccgcgccgag caaggtagct acgcgctggt ggatctcttc
    39481 acccgctacc aggtgacgaa aaacttctcg ttacagggga acgtcaataa cctgttcgac
    39541 aaaacctacg ataccaacat ggaaggttct atcgtctacg gcgcaccgcg taatttcagc
    39601 attaccggca cgtatcaatt ctgaataaca cctgtaaaaa aggcagccat ctggcggcct
    39661 tagtctcccc aacgtcttac ggattagtgg ttacggatgt actcatccat ctcggttttc
    39721 aggttatcgg atttagtacc gaaaatcgcc tgaacaccag aaccagcaac cactacgccc
    39781 gctgcgccca gtttcttcag gccagcctga tccactttag acacatcagc aacgctgacg
    39841 cgcagacggg taatacatgc gtcgaggtta gtaatgtttt ctttaccacc aaatgcagca
    39901 accagagccg gtgccatttc gctggtacct gttgcttttg catcttcagt cgcgtcttca
    39961 cgacccggcg ttttcagatc cagtgcttta atcagcacgc ggaagatggt gtagtaaaca
    40021 atcgcataac cgataccgac gatcgggaac agccacagtt tgctgctgtt accagacaga
    40081 acgataaagt cgatcagacc gtgcgagaac gacgtaccgt cacgcatccc cagaagaata
    40141 cagattggga atgccaggcc tgccagaatc gcgtggatga tgtacaggat cggcgcaacg
    40201 aacatgaagg agaactcgat cggctcggtg ataccggtca ggaacgaggt cagcgccgcg
    40261 gagatcataa taccgcccac tttcgcgcgg ttttctggtt tagcagagtg ccagatagca
    40321 attgcggcag ctggcagacc gtacattttg aacaggaagc caccagacag tttacccgca
    40381 gtcgggtcac ccgccatata acgcggaatg tcgccgtgga aaacctgacc tgctgcgttg
    40441 gtgtattcac caatctgcat ctggaaaggt acgttccaga tgtggtgcag accaaacggc
    40501 accaggcaac gttcgatgaa accgtaaatg ccaaacgcaa ctaccgggtt ctggtaagca
    40561 gcccactgag agaaggtctg gattgcagaa ccaatcggcg gccaaatgaa ggacagcaca
    40621 acgccagtaa agatggcagc caggccagaa atgatcggca caaagcgttt accggcaaag
    40681 aagccaagat actcaggcag cttaatacgg tagaaacggt taaacatgta cgctgcgatc
    40741 gcaccggaga taatccctcc gagtacgcca gtatccgcca ggtgtttaga ggcgatttct
    40801 tcagcaggta aatgcagtac cagtggcgca accacggcca tggttttaac catgatgcca
    40861 taggcaacaa ctgcggccag cgcggatacg ccatcgttat tggtaaagcc gagggcgaca
    40921 ccgatcgcaa aaatcagtgg catgtttgca aagacggaac cgcctgcttc tgccataaca
    40981 tgcgatacaa cggcgggcag ccagctgaaa ttcgcggaac cgacgcccag cagaatacct
    41041 gcgataggca gtacggatac cggcagcatc agcgatttac cgaccttttg caggttagca
    41101 aatgcattct taaacataat tgagagtgct cctgagtatg ggtgcttttt ttacgttctc
    41161 acgcgtggca aggggggaga gcctcgccgt gtacagggca tctaagcgcc ctttatttat
    41221 tacacagagt aaaataattc agtgccgatt tgtttgacgg ctatcacatt tcaacttcag
    41281 atcattggag aagttgcaca ttttgacaaa acatatgtca caatatttca aacgtggtta
    41341 ttcaccgccc aattttcggc ggtgaacttt actcgtttta gccattaatt acgagcttta
    41401 aaaaaactca ttcaacggat ggattgaagg cgggaagcgt cgatgtgaaa cagacgggcg
    41461 aagttatcgg tggttacctg cgccagttct tcaacggcaa cacctttcaa cacagccatg
    41521 tattctgcaa cgtcacgaac catcgcaggt tgattctctt ttcctcgatg cggtaccggc
    41581 gcaaggtaag gtgagtcagt ttccaccagt aaccgatcca gggggacata acgcgcagca
    41641 tctcgaagtt gctccgcatt acggaaggtc acaatgccgg aaaaggagat gtaaaatccg
    41701 agatccagta atttacccgc cgtttctctg tcctctgtaa aacagtgtag tacgccaccg
    41761 caatccgtca ctttttcttc gcgcagaatt gccagcgtat cggcgcgggc gtcacgggta
    41821 tggacgataa ccggcttgtt cagttcacga ccaatctgga tatgatggat gaaggactct
    41881 tgctgacgaa ctttagtttc cggtgtgtaa taataatcca gcccggtttc acccagcgct
    41941 acaacaccct cttctgccgc cagacggcgt aaatcttcta catcgtaggg atcattctgg
    42001 ttgagtggat gcacgccaca agaaaatacg acgttgtcac gttcgcctac cagatcccgc
    42061 atatgtaaat aacccggtaa tgttgtggcg actgccagac aaaatttcac atcgcgtgcg
    42121 gcggctttcg ccagaacgtc atccacgtcc ttatgcaaag attcataatc cagaccatcg
    42181 agatggcagt gtgagtcgac taaaaacatg atgtctctct taaagatgag gaaccggtag
    42241 cacaacgccc ggttgcaggt aatgctcaat acgcagtaaa agatcggtga tgagaagctc
    42301 gcggttgatg cctgtgacag acattaactg ttcacgaatg tggcaaacat cccccagtat
    42361 agcctgcagg cgcgagggag aaagatggtt tgccagttcg acgacaaggc ccggcacatc
    42421 aacattggtc acctgcgcag caccatgatg gcgttttagc gcatccatca gcaacgttgc
    42481 cagccagtgt aaacgcgccg gagcttgttc atgattaagg gccgccagca gcgaatacca
    42541 gtcgcccgat ggcacgctat atgccaatgc ctgacacaat gtttcacgag cctgccagtt
    42601 atctccctga aacaacgcca gtgccgcgcc aggcgaaccg gcgcttaagc gcaatgcggc
    42661 aagtaatgca tcctgtgaca ttgtcacttc gcgtgaaagc caggtcacgg cgtactgttc
    42721 cggcggcggc gcaaggtaat gtaaccgaca acgactacgt aatgttgcca gtaaacgttc
    42781 aggctcgcgg gtagccagga aaaaccaggt ttctgctggt ggctcttcaa gcgttttcag
    42841 caatgcgtta gccgcggcgt cggtcagtaa ggcagcatcg gttacccaga caactttcgc
    42901 accacctagg cgtgcgtgct cattcagctt ttcggtgacc tcacgtactg catcaatgcc
    42961 cagcgtattt ttcccttttt cgggagccag ggtgtagtaa tcgggatgcg tgccagcctg
    43021 catcaactga catccacgac agtgaccgca acttttgtgg ccctgcggtt gttggcagag
    43081 taaataacgg ctcagggcgt agattaaagc atcatcgccc atgcccggta acgcctgaat
    43141 gagtagcgca tggtgacctc ttccggcctg atagctggct accagttttt cgaaatcagg
    43201 tcgtaaccat ggataccatc tcatgcgtcc aactccttca cccagtgggt cacggtagtg
    43261 cggattgcat ccatcacggc ctccagcggc tgggtggcat caatggtatg aatactttta
    43321 tcttgtgcag ccagttccag atagcgggcg cgggtgcgat taaagaaatc gaaagactct
    43381 tgctcaatac gatccagctc gccgcgcgca cgcgcgcgtt ttaagccaac ttccggggta
    43441 acatcgagat agagcgttaa gtcggggcga aaatccccga gaacagcatc acgcagtgtt
    43501 gccagcatat gttggtcaat accacgtcct ccgccctgat acgcctgagt ggagagatcg
    43561 tggcgatcgc caatcaccca ggtgccgtta gccagagctg gtttgatgac cgtttctacc
    43621 agttgaacgc gcgcggcata aaacatcaga acttcggctt tatcggtaat gacttcatcg
    43681 cctaccgatt tgatatccag caccaggctt cttaactttt cggcaagttg cgtaccgcca
    43741 ggttcccgag tgaaaaccat gtcgcggata cccagttgct caagcgtctc aaccaccaca
    43801 ttacgcgcgg tagttttgcc tgcgccttcc agcccctcaa tgacgatata cttactgcgc
    43861 atttttttcc ttaagcactt tcagataatc ctgcacagac ttgttatggc tggcaagatt
    43921 ggtattaaac gtgtgaccac ctttaccatc ggccacaaaa tagagatacg gcgtttttgc
    43981 cggatgcgca gcagccttca gcgaatccgc ccccggcgta gctatcgcac ccggcggcaa
    44041 accggtaatg gtataggtgt tatacgctgt cggcgtttcc aggtctgcac gagaaagttt
    44101 gccattataa cgctctccca tcccgtaaat cacggttggg tcggtctgca ggcgcatacc
    44161 aatgcgtaaa cggttgataa ataccgaggc aacctgatcg cgttcactgg caacggcggt
    44221 ttctttttcg ataattgatg ccatcgtcac cagctgattt ttatctttat aaggcagacc
    44281 gtccgcacgc ccttcccagg cgctatcgac cgctttcacc attttcttgt gcgctcgctt
    44341 gagtaacgcg acatcggtgg tattggcggt atacatccag gtgtctggcc agaaccaacc
    44401 ttcaatccac tccgggtttt ccagttcaag tgcctgcgct acggtggcgt acttatcgtc
    44461 actcagcgtg tgcttgatat acggagcctc acgcaattgc ttgaggtagt cgctcagacg
    44521 catcccttct accagtcgca gcgggaactg tgcttcttta ccgctttcca gcaatttcag
    44581 catctcgcgc acggtcatct gcggtgtaaa gcggtaagtc ccggctttaa agtgagaaag
    44641 atccggttcg atacgcagca accattgaaa aacccgtggg cgattgatga tcttatcggc
    44701 ataaagctgt tcaccaagcg ccagacgtcc ggtccctggc ttcagggtaa atatcgtctc
    44761 ttctttgata agcaatttgc tgtcggcaag atggcgaacc ttccagacgc ccacaccagc
    44821 ggcgataccc agtaccacca gcaataacaa gataatcaat aacacttttt tcatgactaa
    44881 ttcgggcgct cacaaagtgg ggctaaatat tcatataacg ttgctgacga aaaggagaca
    44941 tcgccacagg cacgtacggg catcactggc attaacgcat tacaaataac catctcatct
    45001 gcctgcaacg cctcttccag agaggcttgc acttcgacaa gctgataaga ggattgtgcc
    45061 agcaaacgga tacagaattg tcgcataatg ccgttaacac ctgcctgatc cagacgcggc
    45121 gtataaacta cattgccctt ccgccagaac aaattagccg cacagcattc cgtaacccac
    45181 ccttcgctgt caaggaccag cgcctcatca gcgtttgtct gctcaagatg agagcggatc
    45241 aatacttgct caagccgatt aagatgttta atacctgcaa gatgaggatt gcgccccagc
    45301 cgcaccgggc ttagcgccaa cgtcatccct tcgttacgca aacggtcgta atgtgcagga
    45361 taaaccgtaa cggagagaat ccgcgttgcc ggtccactgt tcaatgtgct gtaccctcgc
    45421 ccgccactac cgcgactgat cacgactttc agcacaccat tttgctgttc tgctgccagc
    45481 gttttcatct cttgttcaag ctgaggccag aagtcacaga aaatcatcaa ccgctgacaa
    45541 gcatcctgta gtcgctggat atgcgccgat aacaaactga ctttaccgtc gataactctg
    45601 gcggtggtaa aacaaccatc accaaactgc gttgcccgat cgcttactgc cagcgattcc
    45661 tgcttatgac cgttaattaa gaacataccg gctccttatc agagaattgt tagtgtggca
    45721 gcatgttcac tacggaacaa gtcggaataa aagctaagaa aaaaggcccg caagcggacc
    45781 ttttatatgg gtgggaaatg acaacttaga tctttttaaa gatcaaagaa ccattagtgc
    45841 caccgaagcc gaaggagtta cacagcgtgt attccattcc gctgacctga cgtgcttcat
    45901 gcggtacgaa atccagatcg caaccttcat ccgggttatc caggttgatg gttggcggaa
    45961 cagcctgatc gcgcagcgcc aggatggagt agatagattc tactgcaccc gccgcaccta
    46021 acaggtgacc ggtcatagat ttcgtggagc ttaccaacac acggcttgca gcttcaccga
    46081 agatggtttt caccgcctgc gcttcagctt tatcgccagc cggcgtagaa gtaccgtgcg
    46141 cgttaacgta gccaatctga ctcgcttcaa tgcctgcatc acgcagagca tttgccatcg
    46201 ccagagctgc gcctgcgcca ttttctggcg gtgacgtcat atgataagca tcgctgctca
    46261 taccaaagcc gacgagttca gcgtaaattt tcgcaccgcg ttttttcgcg tgttcgtact
    46321 cttcaagtac cagcataccg gcaccatcgc ccagtacgaa gccatcacgc tctttatccc
    46381 acgggcggct cgccgcttgc gggttatcat tgcgggtaga taatgcacgt gccgcgccaa
    46441 aaccaccaac acccagcggc gtactggctt tctctgcgcc acctgcaacc atcacgtcag
    46501 catcgccata cgcgataata cgcgcagcat ggccaatgtt gtgcacgccg gaagtacagg
    46561 cggtcgcgat agagatgctt gggccacgca ggccatacat gatagtcaga tgacctgcca
    46621 ccatgttcac aatcgttgac ggaacgaaga atgggctgat cttacgtgga ccaccgttca
    46681 tcagagatgt gtggttttct tcgatcagtc cgaggccgcc aatcccggag ccaattgcgg
    46741 caccaatgcg ggttgcgttc tcttccgtta tatcaaggcc agaatcctgc atggcctgaa
    46801 cgccagcgac aattccatat tgaatgaagg catccatctt gcgctgttct ttgcgcgaga
    46861 taatgtcctc acagttaaaa tcctttacta agccagcaaa tttcgttgca taggcgctag
    46921 tatcgaaatg gtcgattagg ctgatgccac tctgaccggc aagcagagct ttccaggtag
    46981 actctacggt attgccgaca ggagacaaca tgcccagtcc ggtcacaact acacgacgct
    47041 tagacacgtt tgtcctccag ggagggaaaa aatgattcta gtgggacaaa aagataaaac
    47101 tcaggcggtc gaacgaccgc ctggagatgt tcacttacgc ctggtggccg ttgatgtaat
    47161 caatggcagc ctgaacggtg gtgattttct cagcttcttc gtccggaatc tcagtatcaa
    47221 actcttcttc cagagccatt accagctcaa cggtgtcaag agaatccgcg cccaggtctt
    47281 caacgaaaga agcattgttg gtaacttctt cctgcttaac gcccagctgt tcgccgataa
    47341 ttttcttaac gcgttcttcg atagtgctca tactcttaaa tttcctatca aaactcgctt
    47401 tcgcgatggt tttcgtagtg tataaaatgt tgaaaaattt gcaactaaat cccggcaggt
    47461 cttaccacga ttttacgtta ttttgaggcc ttacccccca ataacgcaaa tcattttgca
    47521 ctaattatta ctcataacca cgcagactgc gcgcaacatg agcattttgt gcaaatcgcg
    47581 gtcagaccat gtacatcccg ccgttcacat gcaaagtttc acccgtgatg taagctgctt
    47641 cgtcggatgc caggaatgca accgcgttgg cgatttcctg tgcgccgccg aggcgacccg
    47701 caggaacctg cgccaggata cccgcacgct ggtcatcgct cagcgcacgt gtcatgtccg
    47761 tttcaataaa gcccggagca acaacgttta cagtaatacc gcgtgacgca acttcgcgcg
    47821 ccagtgattt actgaagccg atcaagcccg ctttcgccgc agcgtagttg gcctgaccgc
    47881 catttcccat ggtaccaacc acagaaccga tagtgataat acgaccatga cgctttttca
    47941 tcatagcgcg cattaccgct tttgacagac ggaaaacaga tgaaaggttg gtttcgataa
    48001 tatcgttcca ctcttcatct ttcattcgca ttaacaggtt atcacgagtg ataccggcat
    48061 tattgaccag gatatccact tcaccaaatt ctgcgcgaat tttttccaga acagattcga
    48121 tagatgccgg gtcggtcaca ttcaacatca gacctttgcc gttggcacct aaataatcgc
    48181 tgatcgcctg agcgccattt tcactggtcg cagtgccaat aactttcgcg ccacgggctg
    48241 cgagcgtttc agcaattgcg cggccaattc cgcggcttgc accggttacc agtgcgattt
    48301 ttccttcaaa attcatgatt ttcctctttt aaagctcaag cgccgctgcc atcgctgaag
    48361 gttcgttcag cgccgaggcg gtcagggtgt cgacaatgcg tttcgtcagg ccagtaagca
    48421 ccttgcccgg gccgacttca tagagatgtt ctacgccttg cgctgccatg tactcaacag
    48481 acttcgtcca ctgaaccggg ttatacaact gacgtaccag tgcgtcacgg atggcatcac
    48541 cattggtttc gcatttcaca tcaacgttat tcacaacaga aactgttggt gcgttaaagg
    48601 tgattttcgc taattctact gccagtttgt cggctgctgg tttcatcagc gcacagtgag
    48661 acggtacgct cactggtaac ggcagagcac gttttgcgcc cgcagcttta caggcagcgc
    48721 cagcacgctc aaccgcttct ttatgaccgg caataaccac ctgtcccgga gagttaaagt
    48781 ttaccggaga aacgacctga cctgctgcag cttcttcaca cgctttcgca atagacgcat
    48841 catccagacc gatgattgcc gccatagcgc ccgtaccttc cggtacggct tcttgcatga
    48901 gcttgccgcg catctcaacc agacgcaccg catcagcgaa atcaatcaca ccagcgcaaa
    48961 ccagcgcgga atattccccc aggctgtgac cggccatcat tgccggtgct ttaccgccct
    49021 gctgctgcca tacgcggtac agcgcaacag aggcagtcaa cagcgctggc tgggtttgcc
    49081 aggttttatt cagttcttca gctggcccct gctgggtcag cgcccacagg tcgtagccca
    49141 gcgccgcaga agcttcagca aacgtttctt cgacaattgg atagctcgcc gccatatcag
    49201 ccagcattcc aacggtttga gaaccctgtc cagggaacac aaatgcaaat tgcgtcatgt
    49261 tttaatcctt atcctagaaa cgaaccagcg cggagcccca ggtgaatcca ccgccaaagg
    49321 cttcaagcag aaccaactgc cccggcttaa tgcgcccgtc gcgtacagct tcatccagcg
    49381 cgcacgggac agaggccgca gaggtattac cgtggcgatc cagcgtcacc acgacattgt
    49441 ccatagacat accgagtttt ttcgccgttg cactgataat acgcaggtta gcctgatgcg
    49501 gaaccagcca gtccagttga gaacggtcaa gattatttgc cgccagcgtc tcatcaacga
    49561 tgtgcgccag ttccgttacc gcaaccttga agacttcgtt gcccgccatc gtcagatgaa
    49621 ttgaattctc tggattcaca cggtcagcat taggcagcgt cagcaactca ccatagctac
    49681 cgtcggcatg cagatgggtg gagatgattc ccggctcttc agaggcagcc agcaccgcag
    49741 cgcccgcgcc atcgccaaaa ataataatag tcccacgatc ggttggatcg caggtgcgcg
    49801 ccagtacatc ggaaccgacg accagagcat acttcaccgc cccagatttc acgtattgat
    49861 cggctacgct taatgcatag gtgaaacctg cgcaggctgc tgcaacgtca aatgccgggc
    49921 aacctttaat gcccagcatg ctttgaatct gacaagctgc gctcgggaaa gcgtgcgtag
    49981 cagaagtcgt tgccacaacg atcaggccaa tctggtcttt ctcaatgccc gccatctcaa
    50041 ttgcgcgtgt cgccgcttca aagcccatgg ttgaaacggt ttcgtttggc gcggcaatgt
    50101 ggcgttcgcg gataccggta cgagtgacaa tccactcgtc agaggtgtcc accatttttt
    50161 ccaaatcggc gtttgtccgc acttgttcgg gcagatagct gccagtacca ataatcttcg
    50221 tatacatgta cgctcagtca cttttcggtt atataccgtc acttgcaaac tgcgagttcg
    50281 ctggcagcgt cctgctaccg cagagttccg cttttgccac cgtccagcag ctcaaaacca
    50341 gctgggtata cagattccag gcgagcggca attcgctgag gaacttgtcg ctgcaccgcc
    50401 tgcactgcct gttcaatcgc gaccgcaaaa gctcgctgat tggctgcacc atgacttttt
    50461 atcaccgtgc cgcgcaatcc taacagacag gcgccgttat actggtcggg gttgaggtga
    50521 ctgaatcgcc tcgtcaggct cttttgtagc caacgcttta ataacagtag ccaccacgac
    50581 cgttttttcc cttcaccctg agatttcagc agagaaagga acatcctgac aacaccttcc
    50641 atcgtcttta atgtgacatt tcctgtaaag ccgtcacaaa ccagcacatc tgtcttgcca
    50701 gttaacaact cattggcttc aagatagccg atataattga tagaagggat tgttttaagc
    50761 accgctgagg catcccgaat actgtcgaga ccctttactt cttcttcacc aatattgagc
    50821 aacgccacgc gaggattggg aatttccacc acctcttcag ccagaactga gcccataatg
    50881 gcaaattgca ccaacattgt actgtcacaa tcgacgttgg cccctaagtc aaggaccacc
    50941 gttttgccct tttgctgatg tggtaatacc gtcaccagcg ccggacgctc aatcccctcc
    51001 aggggcttga gtaataattt tgccagcccc atcagtgccc cggtattacc ggcactgaca
    51061 caggcttgcg ctcgaccttc tttcaccagc tccagggcca cgcgcattga actcccacga
    51121 ctggcgcgga tagcttgcga aggccgggca tcactggcga taactgactg cgcaggaata
    51181 atctgcagac gcgaacgttg ttcaaagtca gctttagcaa gtaatggcgt gatggcgtcg
    51241 ggattgccga ctaaaagaag agtgagttgc gaattagaat tcagtgcctg caatgctgca
    51301 ggcactgtca cggaagggcc aaaatcccct cccatgacat ctaacgccag ggttagacgt
    51361 gtcaaggtat cgtcgccgcc cggtttggtc tttccccagt tgcctgggga aaatcctcac
    51421 taagcttcat cacgcagacg cgtgattact tagcgatgac cttgcggccg cggtagtaac
    51481 cgtcggcagt gatgtggtga cgcaggtgtt tttcaccaga agttttgtct acagacaggc
    51541 tggtgactgc ggtcagcgcg tcatgggaac gacgcatgcc acgtttggaa cgggttggtt
    51601 tattctgttg tacggccatg gaccttactc ctcaattacg gtttatcccc gatccaacgg
    51661 ggagcaccaa ttacttacgc tttaagctgg ctaatacggc aaatgggttt ggcttttgca
    51721 cttcttcagg cagttcacca aagaccatgt ccgcttcgga cacttcacag tgttcagaat
    51781 catgcaccgg aactaccggc aaggcgagga tgatttcatc ttcaaccatt gcaagcagat
    51841 cgatttcacc gaattcgtta acctcaatcg gttcatacgc ttccggcagt gcttcagcct
    51901 gttcgtctga acgcacagga ctaaaacaat acgttgtgta gacctgatga gtaaacggct
    51961 tcccgcaacg ctgacactcg agcgttaccg tcaccttcgc atcgccgttt aacactgcga
    52021 gacgttggtt atcgatagcg aacgacatgg agcattccac atcactgtcc acactgacta
    52081 cggattcggc gacgcgctca acctgatcag gggtatagat accctggtaa tcaaggcgtt
    52141 tttgagccgt acgaaccgga tcgagagtca ggggtaattt taccttttgc atagggcgcg
    52201 catattaact ttgtaacgtc atagagtcaa agaaaaaggc agcctgtggt tgccttttgc
    52261 caataattcg cacacattgc gggttactgc tttattttcg ttcagtgctg ataacgaaca
    52321 tcaacctgca aaacgaagcg catagtttaa aatggctatc atcaatacgc tatatctgat
    52381 ggaaaaaaat atgcctaaac ttattttagc ctccacctcg ccatggcgtc gcgctcttct
    52441 ggaaaaactg caaatctctt ttgaatgtgc agcaccagag gtcgacgaaa ccccacgcag
    52501 cgacgaatca ccgcgacagt tggtgcttcg actggcacaa gaaaaagcgc aatctctggc
    52561 gtcacgttat ccggatcatt taattattgg ttcagaccag gtatgtgttc ttgatggcga
    52621 aatcaccggt aaaccgttaa cggaagaaaa tgcccgtctg caattacgca aagccagcgg
    52681 caatatcgtc accttctata ctggactggc gttgtttaat tcggcgaatg ggcatctaca
    52741 aacagaagtg gagccttttg acgtccattt ccgtcacctg agcgaggcgg agattgataa
    52801 ttacgtgcgt aaggagcatc ccctgcactg cgcgggtagc tttaagagtg aaggatttgg
    52861 cattacgctg tttgagcgtt tagagggacg tgaccctaac acgctggttg gtttaccgct
    52921 aatcgcgctg tgtcagatgt tacgtcggga agggaaaaac ccgctgatgg gatagttgtt
    52981 tgcgctggat gcggttacgt cgcatccggc aatcggtgca ggcggcctga tgcggcgtaa
    53041 acgccttatc aggccaaccg cccattatca ggcttatatg cttgtattag cgcgcgttac
    53101 gcagcttttg caaacaacgc ttcaaacctt catccatcgg cgcttcgata cgcatcacct
    53161 caccggtccc cggatgggta aacttcaacg ctgcggcgtg caggaacaga cgatttaatc
    53221 ccgtgcctgc ttcagtgagc tgtctgtcaa attcacggtc accgtagcga tcgtcaaagg
    53281 caatcggatg acccgcatac tgtgtatgca cacggatctg atgagtacgt ccggttaccg
    53341 ggctacaacg caccagggtg gcaaatgcat agcgttcttc cactttaaag cgtgtttccg
    53401 acggtttgcc ttcctgactg acacgcacga tacgttcgcc gctttgcaga atatttttca
    53461 ataacggcgc ttgaacgctc ttcacatgcg actgccactg accgcgcacc agcgccaggt
    53521 aatctttttg cattcctttt tcacgtaatt gctcatgcag agaacgcaac gccgagcgtt
    53581 ttttcgctat cagcaacaca cctgaagtgt cccggtcgag acgatgaacc agttcaagga
    53641 accgcgcttc cgggcgcaac gcccgcaaac cttcgataac gccgaagctt aaaccactgc
    53701 cgccatgtac cgccgtaccg gaaggtttat tcagcaccag gatatgatca tcttcatata
    53761 agatgacgtc cgccagcgcc gccacctttt gcagatgtgg cgaaaccgcc tcttcttccc
    53821 gctcagcaac gcgaaccggt gggatacgca cctcatcacc cgcttcgagt ttatattcag
    53881 gcttaatacg ttttttgttc acccgcactt cgcctttacg caaaatacgg taaatcatac
    53941 tttttggtac gcctttcaat tgggtacgca aaaagttatc gatacgttgc cccgcttcgt
    54001 cagcggtgat agcaacaatt tttacggatg gagtctctgt tttcatggtg ggcgattcta
    54061 attagccaac aggattcgcg ccactcattt ttctatgctt atatttagtt ttgcacctta
    54121 ttacttcact acgtgatcac tttattgatg gttattaaac caatcaccag caagaagtga
    54181 aaaaactgtg agtaagcggg tgataaatgg taaaagtcat cttgctataa caaggcttgc
    54241 agtggaataa tgaggccgtt tccgtgtcca tccttgttaa aacaagaaat tttacggaat
    54301 aacccatttt gcccgaccga tcatccacgc agcaatggcg taagacgtat tgatctttca
    54361 ggcagttagc gggctgcggg ttgcagtcct taccggtaga tggaaatatt tctggagagt
    54421 aatacccagt ctgtttcttt gataattgcg ctgtttttcc gcatgaaaaa cgggcaaccg
    54481 acactctgcg cctctttgag ctgacgataa ccgtgaggtt ggcgacgcga ctagacacga
    54541 ggccatcggt tcacacccgg aaaggcgtta ctttgcccgc agcttagtcg tcaatgtaag
    54601 aataatgagt aagttacgat gaaaagaatg ttaatcaacg caactcagca ggaagagttg
    54661 cgcgttgccc ttgtagatgg gcagcgtctt tatgacctgg atatcgaaag tccagggcac
    54721 gagcagaaaa aggcaaacat ctacaaaggt aaaatcaccc gcattgaacc gagtctggaa
    54781 gctgcttttg ttgattacgg cgctgaacgt cacggtttcc tcccactaaa agaaattgcc
    54841 cgcgaatatt tccctgctaa ctacagtgct catggtcgtc ccaacattaa agatgtgttg
    54901 cgtgaaggtc aggaagtcat tgttcagatc gataaagaag agcgcggcaa caaaggcgcg
    54961 gcattaacca cctttatcag tctggcgggt agctatctgg ttctgatgcc aaataacccg
    55021 cgcgcaggtg gcatttctcg ccgtatcgaa ggcgatgacc gtaccgaatt aaaagaagca
    55081 ctggcaagcc ttgaactgcc ggaaggcatg gggcttatcg tgcgcaccgc tggcgtaggc
    55141 aaatctgctg aggcgctgca atgggattta agcttccgcc tgaaacactg ggaagccatc
    55201 aaaaaagccg ctgaaagccg cccggctccg ttcctgattc atcaggagag caacgtaatc
    55261 gttcgcgcat tccgcgatta cttacgtcag gacatcggcg aaatccttat cgataacccg
    55321 aaagtgctcg aactggcacg tcagcatatc gctgcattag gtcgcccgga tttcagcagc
    55381 aaaatcaaac tgtataccgg cgagatcccg ctgttcagcc actaccagat tgagtcgcag
    55441 atcgagtccg ccttccagcg tgaagtccgt ctgccgtccg gtggttcgat tgttatcgac
    55501 agcaccgaag cgttaacggc catcgacatc aactccgcac gcgcgacccg cggcggcgat
    55561 atcgaagaaa ccgcgtttaa caccaacctc gaagctgccg atgagattgc tcgtcagctg
    55621 cgcctgcgtg acctcggcgg cctgattgtt atcgacttca tcgacatgac gcctgtacgc
    55681 caccagcgtg cggtagaaaa ccgtctgcgt gaagcggtgc gtcaggaccg tgcgcgtatt
    55741 caaatcagcc atatttctcg ctttggcctg ctggaaatgt cccgccagcg cctgagccca
    55801 tcactgggtg aatccagcca tcacgtctgc ccgcgctgct ccggtaccgg taccgtgcgt
    55861 gacaacgaat cgctgtcgct ctctattctg cgtttgatcg aagaagaagc gctgaaagag
    55921 aacacccagg aagttcacgc cattgttcct gtgccaatcg cttcttatct gctgaatgaa
    55981 aaacgttctg cggtgaatgc gattgaaacg cgtcaggacg gtgttcgctg cgtgattgtg
    56041 ccaaacgatc agatggaaac cccgcactac cacgtgttgc gcgtgcgcaa aggggaagaa
    56101 acgccgacct taagctacat gctgccgaag ctgcatgaag aagcgatggc gctgccgtct
    56161 gaagaagagt tcgctgaacg taagcgtcct gaacaacctg cactggcaac ctttgccatg
    56221 ccggatgtgc cgccagcgcc aaccccagct gaacctgccg cgactgtcgt agccccagca
    56281 ccgaaagctg caacggcaac accagcagct cctgcacaac ctgggctgtt aagccgcttc
    56341 ttcggcgcac tgaaagcgct gttcagcggt ggtgaagaag ccaaaccgac cgagcaacca
    56401 acaccgaaag cagaagcgaa accggaacgt caacaggatc gtcgcaaacc tcgtcagagc
    56461 aaccgccgtg accgtaatga gcgccgcgac acccgtagtg aacgtactga aggcagcgat
    56521 aatcgcgaag aaaaccgtcg taatcgtcgc caggcacagc agcagactgc cgagacgcgt
    56581 gagagccgtc agcaggctga ggtaacggaa aaagcacgta ccaccgacga gcagcaagcg
    56641 ccgcgtcgtg aacgtagccg ccgccgtaac gatgataaac gtcaggcgca acaagaagcg
    56701 aaggcgctga atgttgaaga gcaatctgtt caggaaaccg aacaggaaga acgtgtacgt
    56761 ccggttcagc cgcgtcgtaa acagcgtcag ctcaatcaga aagtgcgtta cgagcaaagc
    56821 gtagccgaag aagcggtagt cgcaccggtg gttgaagaaa ctgccgctgc cgaaccaatt
    56881 gttcaggaag ctccagctcc acgcacagaa ctggtgaaag tcccgctgcc agtcgtagcg
    56941 caaactgcac cagaacagca agaagagaac aatgctgata accgtgacaa cggtggcatg
    57001 ccgcgtcgtt ctcgccgctc gcctcgtcac ctgcgcgtga gtggtcagcg tcgtcgtcgc
    57061 tatcgtgacg agcgttatcc aacccagtcg ccaatgccgt tgaccgtagc gtgcgcgtct
    57121 ccggaactgg cctctggcaa agtctggatc cgctatccaa ttgtacgtcc gcaagatgta
    57181 caagttgaag agcagcgcga acaggaagaa gtacaagtgc agccgatggt gactggggtc
    57241 cctgtcgccg ccgctgtcga acctgttgtt agcgcaccgg ttgttgaaga agtggctgaa
    57301 gttgtagaag cccccgttca gattgccgaa ccgcaaccgg aagtggttga aacgacgcat
    57361 cctgaagtga ttgccgccgc agtaactgaa cagccgcagg tgattaccga gtctgatgtt
    57421 gccgtagccc aggaagttgc agaacacgca gaaccggtgg ttgaaccgca ggaagagacg
    57481 gcagatattg aagaagttgc cgaaactgct gaggttgtgg ttgctgaacc tgaagttgtt
    57541 gctcaacctg ccgcgccagt cgtcgctgaa gtcgcagcag aagttgaaac ggtagctgcg
    57601 gttgaacctg aggtcaccgt tgagcataac cacgctacgg tgccgatgac gcgcgctccg
    57661 gcaccggaat atgttccgga tgcaccgcgt cacagtgact ggcagcgccc tacttttgcc
    57721 ttcgaaggta aaggtgccgc aggtggtcat acggcaacac atcatgcctc tgccgctcct
    57781 gcgcgtccgc aacctgttga gtaataatta gctcaagtaa tcaagccctg gtaactgcca
    57841 gggctttttt atttcatctt tgaatcaaca tattaaaatt catcgaatgg catccttgct
    57901 aaccaacaat gcaaaatagg caaaaaaatg cccgccccgg cgatcccaga gcaggcagac
    57961 aaaacatacc cagtttcatg atatgtttta aagcgaaatt atttgttgag ctggaagagc
    58021 gacaatccct gcatatcggt aaatgcttta tacgatgcct gcaatgccgt ttgctgcatg
    58081 atgtaagatg aaatagttgc attccagtcc acatcaacca gatcgctcat ctgctgcgtt
    58141 tgccctaaag cgcgatcgct acctaatgaa tccagcgact ccagttcgtt cagctgcgtg
    58201 cctaattccg cgcgaacggt cagcacattg ttcagcgagt ttttcagtcc gcggttggtt
    58261 ttatctaacg ccgcagcggc ggtttcttta tccgcttcgc tatccgcgac cggcgttttc
    58321 agggctgcga tggcgctatc cagcatggca aaaagattgg tttcagaagc gctaccgtct
    58381 ggttccgcta ccgcgttact ggtaatactg tcgaaaattt tgtcgcccgt atgtcctatc
    58441 accatcgaac gcgaagcatc gacctgttgc ttaatacttt ctgttccgcc gacataatca
    58501 ccgttagcct cactaaacgg cgcagtctct gttttataac cggcaaaaat gtagcgccca
    58561 ttaccgtcag tggtgttcgc cagattcagc aactggtcac gaagcccctg aatatccgta
    58621 gccagcgagg cccggtcatc gtcactcaag gtgccattgc tggcgtagac aattttttcc
    58681 tgagcattct ggatagcagt ggtgacctgg ctaagtacac tctcttccag tgacactttt
    58741 tgagtggcga aagtacgcgc cagcgtgtac tggctgtttt gcgcctgtgc ctgggagaga
    58801 acaaccgctt gtgatgcagc aatgggatca tctgaagggt taacgactcg cttacccgtc
    58861 gacatctgtt cgccgtactt catccattct gcctgagaat tggtgatacc acgcatgttt
    58921 tgctggtaca tcatctgggt actgaaacgc attttatctc cccttagcga atgttaatca
    58981 gcgcatcaaa aatcgcgttt gccgtctgca gaacctgcgc attcgccagg taatactgct
    59041 gaaaacgttg cagatttccg tactcctcat cgagattgac accggaaatc gactgctgct
    59101 gattggaaag ctgcgtcacc acattacctt gcgtggtgct actggttttc aacgtcgcgg
    59161 ttttattacc gatatcactc actaacgagg cataagcgtc gttaaaggat ttcgcaccgc
    59221 ccaccgtttt actattgctt tgcagatcca gcagggcctg accgttgcgg ttatcgctat
    59281 cacccgcatc tttttcgctc gccatcgcta ttttcgcttc gtcggtgatt aatacatcca
    59341 tgttgacgat ggcgtcactt actggtttca gcgtgaagct gtcgttaacg gcaggcgttc
    59401 ctgtaaacgt caactccaga ccatcaaatg ccactttacc gttggcatcc ggcgtcaccg
    59461 taaaagtggt attgctggca aggcgggtga cctgccactg attattatcg aacgagattt
    59521 tgtaatctgt cgccagtacc acggaggcat cagttaccgt ggcaccgatc gcaacgtcac
    59581 ctttgttttt cgtgttttgc agaaccgcgg gcttaccgat agtaaagaaa tcttcaccag
    59641 catcgccgtt agcatcaaat ccggctttgt gttgggtgtt gaaagcctcg gcaaatgcca
    59701 gcgccagttg tccaagcgta ttacgcgtct ggtccagctc ctgagaacgg aatgtcagaa
    59761 tgccgcccag cgacccggta ttcagtaatt tctccgggat ctcaatattg cctgccgtcc
    59821 catcaacata agcgacagtc gtacgagaag ggtcagcgct ggaaggaact gccgccagtt
    59881 gccgcgccgt acttccctga accagtgagt aaccattggc caccgtgatg ttataagtgc
    59941 cgccatcctg aacgctgact tctacaccaa caatctggtt taattcgctc accagttgat
    60001 cgcgttgatc cagcagattg ttaggtgacg cccctgcccc cacgcctgtc aggcgcgaga
    60061 tttgatcgtt caggctggca atttgtttag cgtaattgtt gatctgatca acgctggcac
    60121 ctatcgcgat attgacctgt ttgtcctggt cgcgcagata ttgatcggtg gttttaaact
    60181 gattcaccaa cccttctgat ttcccaatca gcgcctggcg cgctgccggg tcttccgcgt
    60241 tactcaccag cgtttgcagg ctggtgaaga aatcctgcat ctgtgttgcc agcgaagagg
    60301 tactggtgga gagcatattg tcgattttcg acatctgctc atagcgggcg gtcaggccgc
    60361 tactttgcgt ctgcgcagca cgtaactggt tggtaataaa cgcatcatac tcacgctgca
    60421 caccagaaac gtagacgcca ttgccaaccc agccgccagc gcccaacgtg ctattggcct
    60481 gcgccataat agtggtttgg cgggtatatc cggcaacgtt atagctggag atattattac
    60541 ttgccgtatt taacgccgcc tgggccgcgt tcagtccgct catggcgtta ttgatcaagc
    60601 tggacatgga ggttccttat aagccttcaa ttacagagta ttatcggcag cgacccgccg
    60661 gacttgagtt attcagaaca gattatcaat gttcatgctg taggttttgc tcaccttgtc
    60721 gcttatcgat ttcatctgct ggatcatgtt ggtgagttta cgggcatagt gaggatcggt
    60781 agcatacccc gcgtcctgta gggcctgcgc cccctgctcc gcactcgcgg ctgtcgtcac
    60841 ggcggcgtag cgcgggttac gtgttaacag cccaacgtaa tccgacaatg cttccagata
    60901 cgagctgtag acccgaaatt tcgcttttac tttcttcgct tcgccatttt catattcagt
    60961 cgtggtgatt tcagtaactg gacctttcca gttgccagag gctttgacgc caaacaggtt
    61021 atagctcggc tcgccgtttt cgcggcggat ttgccgttgc ccccaaccag actccagcgc
    61081 cgcctgagcg aggatcaaat gatgtggcac accgctttgc tggcttgcca gttgggcggg
    61141 cagcgagagt tgcgcgagga atgctttact gtcacccggc agcgaatcat cgtagttacg
    61201 tggcacggcc ttttgcacca gctgcgaaag cgcctgattt tgataacgca ccacagtttc
    61261 gagcgggaat ttcatcggtg ctgctggcgt ggactcctct ggcaatggtt gttctggcgt
    61321 catctgttta accatcatct ctgcaagccc cagacctttg cccgccgtca tctgttgggc
    61381 aatctgctgg tcatacatac tggtatacag gcgagtgtgc tcgctgctga acaggccatc
    61441 ttttggtaaa gcgtcgcgca tgcttttcaa catcatctgc acgaacatcc cttccacctg
    61501 acgggccacc ggacggatat ttgccgccgg atcttcgccc gctttcgcct ttagttcgtt
    61561 gagtgattgc gcatcccagg ccgcacttgc cagtagtttg ctgtcgctga tcatcagatg
    61621 atttccagtt ttgcccgcag acatcccgca ctttgcattg attgcagtat ggacatcaga
    61681 tccatcggcg tagcgcccag cgcattgagc gcgcgcacca cgttattgag gctggcgctg
    61741 gaacgtacgc tttgcagcga accgccgctc tggcgtaaat cgatctgcgt ttgtggagta
    61801 accacagtct gtccaccacc aaacggtgta tctggctggc tgacattggc ctgacgattg
    61861 actgttactg agagatttcc ctgcgctacc gcgcagctgt cgagggtcac ttcgcgattc
    61921 atcaccaccg aaccggtgcg cgagttaatc actactttag cgtcctgcgg ggtgacatta
    61981 acctgcatat tctggatatc tgcaaggaaa cggacctggg aactgttgcc acttggtacg
    62041 cgcacctgaa tagtccgcgc atctaacgcg gtggcgctgc catatccacg cacgcggttg
    62101 atggtgtcag cgatttgctg cgccatgctg aaatcttcgt cgttaagttg caaattaagg
    62161 gtattcccga cgccaaactg gctgggcaat tcacgttcaa taaccgcacc attggtgatc
    62221 cgtccaccgt tcagttggtt cacctgaaca ctgctaccgc cagcggaggc tcctgcgccg
    62281 ccaaccagaa tattgccctg cgccagcgca tacacctgac tgtcaacgcc cttaagcggc
    62341 gtcatcaaca acgtacctcc acgcaagctt ttggcatttc ccatggaaga aaccaccaca
    62401 tcgatggttt gcccctgacg tccaaacgga ggaagtgact ctgtcaccat taccgcagcg
    62461 acgtttttta gctgcatatt ggtgcccgtc ggaacggtaa ttcccagctg tgagagcatg
    62521 ttattaagcg tttgtgtggt aaacggcgtc tgggttgtct ggtcaccggt gccatccagc
    62581 cccaccacca gaccatagcc aatcagtgag ttttgcctta ccccctgaac actggtgaga
    62641 tcgcgaatac gctcagcctg agccgccgtc gtaaccagta gaagaattaa tgcagagaga
    62701 aatttaatca ccacagcctc acttacattg gcgacaggtt aaggaagaaa cgctgcaacc
    62761 agcccatatt ttgcgcttcg ttaatgtagc cattgcctac gtattcaatg cgcgcatccg
    62821 ccacctgagt agacggtacg gtattgctgc cgctgatagt gcgtggatta accacgccag
    62881 agaagcgaat aaattcggta ccctgattaa tggcaatctg tttttcaccc accacatgca
    62941 ggttgccgtt gaccagtacc tggtcaaccg tcaccgtcaa cgtgccgcta aaggtattgc
    63001 tggcattggc cccgcccttt ccgttgaacg tgttaccacc ggaggcttcg acatcggcac
    63061 gagcgttacc aaacagcccc tgcaaatagc gcggcacagt atcaaagcca aaattagttt
    63121 taccgtcacg gctggcattc gcagaggagc ttttgctggc gctgacgttc tcctgcaaca
    63181 cgatggtcag cgtatcgcca atattgcgtg gtcgacgatc ttcaaacagc ggttgatagc
    63241 catagttaat cggctgagca gactggaaaa tagaaccgtt ggcgacgggc gtcggaccgg
    63301 gaaccggttg tgcactggtc gccccctgca ccagcggcgt ggagggtatc caggcgcagc
    63361 cggttagtga aagcaccaac aagctggaaa tggcataagt atgcgcagcg tttttttgca
    63421 tggctatcat cttcaaaaat cagtaaaccg gtgaacctgc caccggttaa gccttagagt
    63481 tgcgtcagtt tttgcagcat ctgatcggtg gtggacaccg ctttactgtt gatttcgtaa
    63541 gcgcgttgca cctgaatcat attgaccagt tcttccgcca cgttgacgtt agacgtttca
    63601 acataccctt gatacagcag tcccgcgccg ttcaggcccg gcgtgctttc gttcggtaca
    63661 ccagaggatt gcgtttcggt gtagaggttt tcgccaatgc tctccagccc ggtgtcattc
    63721 ataaaggtgg tgagattgag ctgcccaacc tgaaccggag ctgcctggcc ttgttgggtt
    63781 acgctgacca cgccatcacg accgatggtg atacttaacg cattcgccgg aatggtgatc
    63841 gctggctgca cctgaaaacc accagctgcc cgttctgatc cacctggaaa gagccgtcac
    63901 gggtataggc tgatgaacca tctggcaaca tcacctggaa aaagccctgc cctttaatcg
    63961 cgacatcttt gctgttgttg gtctgcgaca ggtttccctg gctgtgtaag cgttcagtgg
    64021 cgaccgggcg tacccccgtg ccgatttgta atccggaggg taaggtggtt tgttcggaag
    64081 actgtgcccc cggctggcga atggtttgat aaagcagatc ttcaaacacc gcgcgctgac
    64141 gcttaaaacc gttagtactg acgtttgcca ggttgttggc aatgacgtcc atattggttt
    64201 gctgggcgtc aaggcccgtt ttggcgatcc ataatgaact gatcataaaa tgtcctgtag
    64261 tggattagtg acaaagtgca tatgtcttct tgccggatgc ggcgtaaacg ccttatccgg
    64321 cctacaagtg ctcgcaaaat cgataaattg cgattgcccc catagacccg ataaacttgc
    64381 gcatcgggca acttatactt gtcatgtatc ctttcaatta actcatcgac agcagttggt
    64441 tggcacggcc tgcgttatca tcgacgctgc tgatcacctt catctgcatt tcaaaacgcc
    64501 gcgcgctggc aatcatgtcg ctcattgccg caacggcatt gacgttactg ccttccagaa
    64561 cccccgacat cacacgcaag gttggatctg cctgcagtac cggccctcgc gtggcctggg
    64621 tttctgcgct taaacgaaaa atgccgtcgt caccgcgctg cacttcgctg cccgtggctt
    64681 tcaccagttt cagacgccct actggcgcaa ctgtatttgc cggatcgccc ggattgagcg
    64741 ccgagattgt gccatcggca gcaatagtga tttccgctcc ttccggcaca gcaattggcc
    64801 ccgcctcgcc tatcacctga tgcccctgaa ttgtcagttg cccggtggga tcaacctgaa
    64861 tgctgccatt acgcgtatac ccttcgctgc cgtcagcggt ctgcacggcc agccagccat
    64921 cctgctgcaa cgcgacgtcc agcgggcgcg aggtgtaatc cattttgccg ggcgtcatat
    64981 ctgcgcccgg cgttgacgcc gtgaccaacg tgcgcgtggg cagagaaagc ccttccactg
    65041 gcaccgcgcg taaagcattc aactgcgcgc gaaaaccggg cgttgaggca ttggccagat
    65101 tactggcggt taccgcctgt tgattcagtg tctggctggc tgctcccatc gcggtataaa
    65161 ttgcgtgatc cattgagcta tcccgtcagc gattagcgta agttaaccag cgtgttgagg
    65221 atctggtcct gagttttgat ggtctgggcg ttagactgat agttacgctg ggcaacgatc
    65281 atattgacca gttctttact gagatcgaca ttggacgctt ccaacgcacc gttggtcagg
    65341 gtgccaaagt tgcccgtccc ggctgtcccc aacagcgcca cgccagaaga ttgcgtcgca
    65401 gaccagacgt tgtcgccttc ggatgccaga ccttcgttgt tggcaaagtt cgccagtaca
    65461 atctgcccca gcagttgtgt ttgttcgttg gaatagttgc cgacaaccgt accgtcatca
    65521 ttgatttgat aactcaccag atcgcccggt ttgtagccat tctgggtggt tgccacaata
    65581 ttgttagcgc cggtattttg ctgcatggag ttgaggaagc tcaggctaaa cgtggcggga
    65641 tctgcgccgt taattgcgcc agtggtaata ttctctgttg gatttgaggt cagaacgcca
    65701 ttggcattaa acaccagctt cattgcaggc tcggctgtac ctgttggatc actgctatcc
    65761 tgggtgtaga cctgccagtt attatccccg gtcttcacaa agtagacgct catgtcatga
    65821 gcattaccct gactgtcgaa aacagtcacc gaaccttttt tgttatagct atccgcattg
    65881 ctggcgctga atggcgtaac agtaggaagc ggatcactgg aattcaggtt gatctgcatc
    65941 gacgccgtgg tggtagtttt cgctgccatc agggtattcg ggatcgaaat attggtcgga
    66001 ttcgcccctt gctgaatagt cggcggcgta ccggttgccg ggtaacccgt cagctgtaaa
    66061 ccttgcatat tcaccaggtt acggttttca tccagcttaa attgtccgtt acggctgtag
    66121 aacaccgaac cgttgctgtc taccagacgg aaaaaaccgt tctggctgat agcaacgtcc
    66181 agacctcgcc cggtgttggt ggtcgtgcca tcggtaaagt cctgagtgat accggcaact
    66241 tttaccccca gtcccacttt cgaaccggca aacatatcgg caaaagaggc cgtgcctgat
    66301 ttaaagccgt aggtggcgga gttggcgata ttgttgccaa taacatcgag gttggtggcg
    66361 gcagcgttta atccgctaac cgcttgagaa aaggccatga ctgactcctg aaatgtgaag
    66421 gcttaaatta tctgccgtac ttcgtcgagg gtggtggtgc cgtaagtgcc gagatccagc
    66481 gtattaccgc tgttgccgcg gagcacaccc tgcaccagag caaactgcag cggctgggca
    66541 accagttgtg taccaccgtt actggcgcta atcgctacat tgtaagaacc gttcggcgca
    66601 gtgctgccat cagtcaacgt accgtcccag gtgaaactgt ggactccggc ggtcagttca
    66661 ccaatatcaa tggtgcgcac aaccgcgcca tttttatcgg tgatggtggc cgtaactttg
    66721 tctgccgcct gttgcagctc aacaccaaac ggcgtggtcg tggtcacagc cccttcttca
    66781 ctgccggttc ccgcaagaac agtggtgccg gggatcatca cgccgtgacc gatcaggtta
    66841 ctggcctgta acgactggct gttatcaatc tgtccggaaa tagatccgag cgtggtattg
    66901 agtttttcaa tcccgctgac cgtgctgatt tgtgccaatt gcgacgtcag ctcgttgttt
    66961 tccattggat tggtcgggtc ctggtttttc agctgcgcca ccaacaaagt cagaaaactg
    67021 ctttgtaagt ctgcggcgtt gctgcccgtg agcgaactac tgctggtggt actgacgccg
    67081 gtatttgtcg gatcggtggt ggttaccgca atggacatag ctttctcctt tattgaccga
    67141 gcgtaagggt tttcagcatc atgcttttca ccgtgttgag cacttcaaca ttggcctgat
    67201 agctgcgtga cgccgacatg gtgttaacca tctccccgac gacatcaacg ttcggcattt
    67261 ttacgtagcc ctttgcatcc gccagcggat tacccggttc ataaaccagt ttgtccgggg
    67321 cctgactttc tataacatcg gcaaccttta cgccgcctgt cgcagcacct ggtgcagcgt
    67381 taacctggaa taccacctgt tttgcccgat atggctgtcc atcgggaccg gtcacgctat
    67441 cagcattcgc cagattactg gccgccacgt tcaagcgctg ggactgggca gttaacgccg
    67501 acccggcgat atcaaaaata ttcagcagtg ccatccgtta atttccgctc tgtaaaacgt
    67561 tcatcatgcc tttgatttgc ccgctcaacg cgctaaggct catctggtat tgcaggctgt
    67621 tatcggcaaa ctgggtgcgt tcgcgatcca tatcgacggt attaccgtca agcgaaggct
    67681 ggtccggaat acggtattgc agttctgcgg taggaggcgt cagcgcctgc gccggaatgt
    67741 gttgcgttga ggtcatcgtc agtgcaacca cactggttgc atcccgtcca cgttgcatga
    67801 cttttttaag ttcactggca aaatcgatat cgcgcgcctg ataaccaggg gtatcggcat
    67861 tggcgatgtt tgctgccagc acttcctgac gctgggcgcg cagattgagc gcctcttgtt
    67921 gaaaacgtaa ggcggcgtcg agcttatcga gcatatctcc tccgcaggta tcaaaattct
    67981 gccatcagct taaatgcctt tacgcgcgcg ttatcggcgg aataaacgca aaatgggtcg
    68041 ctatttatgc cgttgatgac cattgcggac aggtacaatt cacgttgtag aaatggctgg
    68101 gggcgaaaat gctggcaata aaacgtagcg tggcgatcat ctcgatacta ttcactccgt
    68161 taagtgcggc gagtaatctc acatcgcaat tgcaaacctt ttttagcgcc cagctcgcgg
    68221 gggtaagtga tgaggttcgt gtttctattc gtacagcgcc caatctacta ccgccgtgcg
    68281 agcagccatt gctttcgatg agcaataatt cccgcctgtg gggcaatgtg aatgtgttgg
    68341 cacgctgcgg taacgacaaa cgatatttac aggttaatgt acaggccaca ggaaattatg
    68401 tggttgccgc gatgcccatt gtgcggggag gaaagctgga agctggcaat gtcaaactaa
    68461 aacgcggacg gcaggatacc ctgccaccgc gtacggtgca ggatatcaat caacttgttg
    68521 atgccgttag cctgcgcgat ctatcacccg atcaacctat ccagttaacc cagtttcgcc
    68581 aggcatggcg ggtaaaagcg ggacaacgcg tcaacgtgat cgccagcggt gacgggttta
    68641 gcgccaacgc agaaggtcag gcgctgaaca atgcagccgt cacacagaat gcgcgggtgc
    68701 gcatggtatc gggactggtg gtcagcggcg ttgttgatgc agatgggaat attcttataa
    68761 accagtaagc tgttaaagat tacccgtccc ttgccgataa ataagcaaca catgataaaa
    68821 gcgccctcaa tgaggaataa accatgagta ttgatcgcac ttcgcctctg aagcccgtaa
    68881 gcaccgttca accacgcgaa accactgacg cgtcggtaac gaacacccgg gcggcaaaaa
    68941 caacagcctc caccagcacc agtgtgacgt taagcgacgc gcaagcaaaa ctgatgcaac
    69001 ccggcagcag tgatatcaat cttgaacgtg tcgaagcgtt aaaactggcg attcgtaacg
    69061 gtgaactaaa aatggacacc ggcaaaattg ccgatgcgct gctcaacgaa gcgcagcaag
    69121 acttgcagag taactgatcg tatgacacgt cttgcagaga tcctcgacca gatgtccgct
    69181 gtgcttaacg atctcaaaac ggtaatggat caagaacagc aacatctctc tatggggcag
    69241 atcaacggca gccagttgca atggattaca gaacaaaaaa gctcactgct ggcgacgctg
    69301 gattacctcg aacagttacg caggaaagaa cccaatacag caaatagcgt tgatattagt
    69361 caacgctggc aggaaattac tgtgaaaacg cagcaactac gccaaatgaa tcaacataac
    69421 ggctggttac tggaaggaca gattgagcgc aatcaacagg cgctggaaat gttgaaaccg
    69481 catcaggaac cgacgctata tggggcgaac ggtcagacct caacaaccca tcgcggcggt
    69541 aaaaagattt cgatctgaat attatctccg gcctgcactg caggccggaa tgcattgtta
    69601 caccgtccgg cgggcaaatt ctttaacttt gaagcccagt accgccagtg cagcgaagta
    69661 cgcggcaatc cccgccagca cgaccgccat taaacgcagt aagcgccagg gcatggtacc
    69721 caatgaccac tccggcatga tatgtaacat acctaaaagc acgccagaca tcaccagtac
    69781 cgccaccacc agacgcaaca gaaacgccat ccagccgggt tgcggagtaa agattttctg
    69841 cttacgcaac tgccagtaaa gcagcgaagc attcagacac gccgccagac caatagaaag
    69901 tgacagccca gcatgtttca acggaccaat aaacgccagg ttcatcaatt gcgttaaaat
    69961 cagcgtaacg atggcaattt tcactggcgt tttaatgtcc tggcgggaat aaaagccagg
    70021 agccaacact ttcactacaa tcaggccaat caaacccacc gagtaggcaa ttaacgcccg
    70081 ctgggtcatc agcgcatcaa acgcggtaaa tttaccgtac tggaacagcg aaacggttaa
    70141 cggacccgaa agaatgccca acgcaaccgc actcggcagc gccaacagga aacaaagacg
    70201 caacccccag tccatcaaac ggttgtattc atcatgattg ccactggcaa aacttttcga
    70261 cagcgacggc agcaaaatgg tgccaagcgc cacgcccagc acaccggagg gaaactccat
    70321 taagcggtcg gcgtaataca tccaagacac cgaaccggaa gcaagaaacg aggcaaaaat
    70381 ggtgttgatg attaaggaga tctggctcac agagacgcca aggatcgccg gtcccatctg
    70441 tttcaccacg cgcattgctc cggcatcgtg gaagttaatg cgcggcagga ccagcatgcc
    70501 gatcttcttc aggtgcggta gctgatacac cagctgcagg atgccgccga ccgtaacagc
    70561 ccacgccagc gccagcaccg gtgggttaaa gtacggtgcg gcaaacagcg cgaaaccaat
    70621 catgctgatg ttaagcagtg ttggcgcaaa cgccggaatc gagaagcggt tccacgtatt
    70681 cagaatcgct cccaccagcg acgccaggga gatcagcaag atatagggaa aggtaatctt
    70741 tagtagctgg ctggtcaggg caaatttgtc agctgtgtca gcgaagcctg gcgcggtcac
    70801 catgatcacc cacggtgcgg cgagcatgcc agcgaccgtc acaaccgcca gcgcaagtgt
    70861 cagcaggcca gaaacataag agacaaagac ccgcgtggcg tcttcaccct gcttactttt
    70921 atattccgcc agaatcggta caaatgcctg ggaaaatgcc ccttcggcaa agatacggcg
    70981 taacaagtta ggaagtttaa aagcgacgaa aaaggcgtcg gttgccatcc ctgcgccaaa
    71041 gattctggcg acaattgcgt ctcgtgcgaa gccaagcaca cgcgaaaaca tggtcatcga
    71101 gctgacggcg gccagcgatt ttaataaatt catcggtgtt ctaatccaga cccacgggca
    71161 aaacgcctgc aaggcaggcg ctgaaagagg cgaatagtct acctggatta tggtgaattg
    71221 ctaccgccag atgttacagg gtttattcac tcatcgcatc gcgccagatc ttgtcaacga
    71281 tacgttgcgc cagcactgcc tgttcgccgg cggtttgcag aactgtctga ttttgcacac
    71341 attcaatgaa gtgccgcgca cagccgacaa atccacgttg ctcaagagtg ctctgccaac
    71401 caggaatcgg tttatgcact acgccctgcc cgcgctcctc acgccattcg cgcatatccg
    71461 taatgtcgat gagcgcatcg tcagtcacgg cctgcacggt ttcacgctga cttccggcac
    71521 ggcgatgcat acaggtggtg atctgcaaag gatcggccga aaaatggtgc tcggcaaaca
    71581 gcatttcgcc agcgtcgtta gtcagtagcg taccgccatc cagagaggct ttgccgcccg
    71641 acaaccacag cgcggtatcc accacatgca gataatcatc cagcaacgtg aaataaagat
    71701 cgtgtggccc gacactattg ctacgatgtt tatccattct tagcgaagct gcggtagcga
    71761 gttgcgtttt taactcaccg tagagtggtg cgaaacgacg gttaaaaccg accatcaacg
    71821 tcagtttttt ccgcgccgcc agttccacca gccgttcagc atcgcgcaga ttttctgcca
    71881 gcggtttatc gacacagaca tgtacacccg cattgagtaa cgtactgacc acgtcaaagt
    71941 ggctggcggt gctggaatgc acaaaaaccg catcgcaact ggcggcaagg ctggataacg
    72001 aatcggcata aggaatgcgc cagctttcac aaattggcag ggctttcgcg cgcgtaggcg
    72061 accaggctcc ttgtaacatc cagtcagacg ctgccgccag caccggtaac cacgcttttt
    72121 gcgcaatgcc gcctaatccc acaacgccga tacgtaattt tttcacttta atctcccaga
    72181 tgggccagca acgaatcaag acgctgtttc agttctgcca cttcgatttc cagggcttcg
    72241 acgcgggcct gtaaatcacc atcaaccgcg ttcgacatat ccgttaccgc cggctgatcc
    72301 tctacctcac cactgaaaag atgcatgtag cggctttcgc gtttacccgg ttcgcgggcc
    72361 agacgcacca caaaaggacc atcttcgcga tttgccagtt gttccagcgt cgactccact
    72421 tccgccatat cgctgaattc atacattcgc gcggcgcggc tgcgcagttc acccggcgtc
    72481 tgggcaccac gcaataacaa cgtggtgatt aacgccactt ccgctgcgct cagtttcaga
    72541 tcgccaaatt ctgaattaca aaaacgttgt tcatatttgg tgacccgatt accaaagcca
    72601 ctcaccgtgc gcagataatg gcgtttgacc agattatcca gttgctcctg cacttcagat
    72661 tcactcagat tcatgaccgg ttcacggttc gttttctgat tacaggccgt gactacacca
    72721 ttgactgaga gcgggtattg ctccggcgtg gtcacctgct tttccagcaa gcaaccaatc
    72781 acgcgagctt caagggcagt aagttgatat ttcatgagat tctccttagc ggccgggcgt
    72841 ccagtctggg gtagttaatg ccgtcagtac gtgatcgcgc cattgtccat caatcaacag
    72901 atagtctttc gcatagcctt ctttttcaaa acccagtcgc gccagtaaat caccgctgcg
    72961 tttattgtgc ggcatataat tagccataat gcgatgaata tgttgggtgc gctgcatata
    73021 acgaatggct gcggtcaggg cttcaaacat gagtcctttg ccctgccatt tttgcccaat
    73081 cgaataaccg agatagcagg catgaaaaga gccacgaaca acattggaaa aattggcaac
    73141 gccaataatc tctttttcgt ccgggtcgaa taagccaaag tagaaagctg aaccttgttt
    73201 atgaaattcg ttaatcatcc ccagcctggc ctgccagcct gatggataac agtggctttc
    73261 gtcgcgcact ggctcccagg gcttgaggaa atggcgattc tctgcgtaat aatccgcaag
    73321 acgccaggca tcacgatcat gcaccagacg cacgaccagt cggtctgtgg ttaagcgcac
    73381 ttttggcacg ttactgcgat agccaaacat tctataccta ctccttcccg taacgtcttt
    73441 aatacgctgt aagtattact atacctgcgc aaccagaccc tgtgaaaagt ccaaaatgcc
    73501 attttctgca ttattcacat atttgataaa aactctctat caaagcgatg ttttgataaa
    73561 aaaatattgt ccccgaagtg atgggaaaag tcttagcggc aatgcagaat agaaagtgct
    73621 catcttttat ttcccgggag gggaaatgtc ccgcgtatcg caggcgagga acctgggtaa
    73681 atatttcctg ctcatcgata atatgctggt cgtgctgggg ttctttgttg tcttcccgct
    73741 gatctctatc cgcttcgttg atcaaatggg ctgggccgcc gtcatggtcg gtattgctct
    73801 cggtctacgc cagtttattc agcaaggtct gggtattttc ggcggcgcaa ttgccgaccg
    73861 ctttggtgcc aaaccgatga ttgttaccgg tatgctgatg cgcgccgccg gattcgccac
    73921 aatgggtatc gcccacgaac cgtggctatt gtggttttca tgcctgctct cgggactcgg
    73981 cggcacgttg tttgatccgc cgcgttcggc gctggtggtg aaactaatcc gtccacaaca
    74041 acgttgtcgt tttttctcgc tgttgatgat gcaggacagt gccggtgcgg tcattggcgc
    74101 attgttgggg agctggctgt tgcaatacga ctttcgcctg gtctgcgcca caggggccgt
    74161 tttgtttgtg ctgtgtgcgg cgttcaatgc gtggttgtta ccggcatgga aactctccac
    74221 cgtacgcacg cccgttcgcg aaggcatgac ccgcgtgatg cgtgacaagc gttttgtcac
    74281 ctatgtcctg acgctggcgg gttactacat gctggctgta caagtgatgc tgatgctgcc
    74341 aattatggtc aacgacgtgg ctggcgcgcc ctctgccgtt aaatggatgt atgccattga
    74401 agcgtgtctg tcgttaacgt tgctctaccc tatcgcccgc tggagtgaaa agcattttcg
    74461 tctggaacac cggttgatgg ctgggctgtt gataatgtca ttaagcatga tgccggtggg
    74521 catggtcagc ggtctgcaac aacttttcac cctgatttgt ctgttttata tcgggtcgat
    74581 cattgccgag cctgcgcgtg aaaccttaag tgctttgctg gcggacgcaa gagctcgcgg
    74641 cagctatatg gggtttagcc gtctgggtct ggcgattggc ggcgctattg gttatatcgg
    74701 tggtggctgg ctgtttgacc tgggcaaatc ggcgcaccag ccagagcttc cgtggatgat
    74761 gctgggaatt attggcatct tcactttcct tgcgctgggt tggcagttta gtcagaaacg
    74821 cgcaacgcgt cgtttgctgg aacgcgacgc ctgatttgat actcccccct gactcctcca
    74881 tactgaaagt gaactgacag ccaaatggag gagtcacgtg aagctataca tttacgatca
    74941 ctgcccttac tgcctcaaag cccgcatgat tttcggcctg aaaaatatcc ccgtcgaatt
    75001 acatgttctg ctcaacgacg acgcagaaac acccacccgg atggtcggtc aaaaacaggt
    75061 tcccattctg caaaaagatg acagccgcta tatgccagaa agcatggata tcgttcacta
    75121 tgtcgataaa ctcgacggca aaccgttact gaccggcaaa cgttcccctg ccattgaaga
    75181 gtggctgcgc aaggtcaatg gctacgccaa caaactgctg ttgccgcgtt ttgccaaatc
    75241 ggcatttgat gagttttcta ctcccgccgc gcgcaaatat ttcgtcgaca agaaagaggc
    75301 cagcgcgggt aattttgccg acctgctggc ccactctgac ggtctgatta agaatatcag
    75361 cgatgatttg cgtgcgctgg acaaactgat cgtcaaaccg aacgccgtga atggcgaact
    75421 ttcggaagat gatattcagc tatttccgct actgcgtaat ctgacgctgg tagccggaat
    75481 caactggcca agccgcgttg ctgattaccg cgataatatg gcgaaacaga cacaaatcaa
    75541 tttgttatca tcaatggcga tttaatcctg acccgccgcg gcagcctgta ttcgcgggtt
    75601 gccgccgttt tcagtttttt ctgctggcga gtttatcgct ggtgacacat gctgcgttgt
    75661 gtcaggatat tgacgcagat ttcatgtcga ggactaccat gaacaagttt ttatttgccg
    75721 ctgcattgat cgtcagtggc ctgctcgttg gctgtaatca actcacccaa tacaccatca
    75781 ccgaacaaga aattaaccag tcgcttgcga aacataataa tttctcaaaa gatatcggtt
    75841 tacccggcgt ggctgacgcc catattgttc tgacaaactt gatcagccaa attggtcgcg
    75901 aagagccgaa taaggttacc ctaaccggag acgccaatct ggacatgaac tccctgttcg
    75961 gtagtcagaa agcgaccatg aaactgaagc tgaaagcgct gcccgtgttt gataaagaaa
    76021 aaggtgcgat cttcctgaaa gagatggaag tggtcgatgc gacggtacaa ccggaaaaaa
    76081 tgcaaacggt gatgcaaacg ttgcttccct atttgaacca ggcattacgc aattacttta
    76141 accagcaacc tgcttacgtc ctgcgcgaag atggcagtca gggcgaagca atggcgaaaa
    76201 aactggcgaa aggcattgaa gtgaagccag gcgaaattgt cattccgttt acggattaat
    76261 gacgagggcg cattcgcgcc ctttattttt cgtgcaaagg aaaacgtttc cgcttatcct
    76321 ttgtgtccgg caaaaacatt ccttcagccg gagcatagag attaatgact gcaccatccc
    76381 aggtattaaa gatccgccgc ccagacgact ggcaccttca cctccgcgat ggcgacatgt
    76441 taaaaactgt cgtgccgtat accagcgaaa tttatggacg ggctatcgta atgcccaatc
    76501 tggctccgcc cgtgaccaca gttgaggctg ccgtggcgta tcgccaacgt attcttgacg
    76561 ccgtacctgc cgggcacgat ttcaccccat tgatgacctg ttatttaaca gattcgctgg
    76621 atcctaatga gctggagcgc ggatttaatg aaggcgtgtt caccgctgca aaactttacc
    76681 cggcaaacgc aaccactaac tccagccacg gcgtgacgtc aattgacgca atcatgccgg
    76741 tacttgagcg catggaaaaa atcggtatgc cgctactggt gcatggtgaa gtgacacatg
    76801 cagatatcga catttttgat cgtgaagcgc gctttataga aagcgtgatg gaacctctgc
    76861 gccagcgcct gactgcgctg aaagtcgttt ttgagcacat caccaccaaa gatgctgccg
    76921 actatgtccg tgacggaaat gaacggctgg ctgccaccat cactccgcag catctgatgt
    76981 ttaaccgcaa ccatatgctg gttggtggcg tgcgtccgca cctgtattgt ttacccatcc
    77041 tcaaacgcaa tattcaccaa caggcattgc gtgaactggt cgccagcggt tttaatcgag
    77101 tattcctcgg tacggattct gcgccacatg cacgtcatcg caaagagagc agttgcggct
    77161 gcgcgggctg cttcaacgcc ccaaccgcgc tgggcagtta cgctaccgtc tttgaagaga
    77221 tgaatgctct gcagcacttt gaagcattct gttctgtaaa cggcccgcag ttctatgggt
    77281 tgccggtcaa cgacacattc atcgaactgg tacgtgaaga gcaacaggtt gctgaaagca
    77341 tcgcactgac tgatgacacc ctggtgccat tcctcgccgg ggaaacggta cgctggtccg
    77401 ttaaacaata aaaatcgtag cttcctgttg tcattaggtt atttaacctg tataaataac
    77461 cagtatattc aacagggggc tattatgcga attgaagtca ccatagcgaa aacttctcca
    77521 ttgccagctg gggctattga cgccctggct ggcgaacttt cccgccgtat tcagtatgcg
    77581 tttcctgata atgaaggcca cgtatcggta cgttatgccg cagcgaataa tttatcggtt
    77641 attggcgcaa caaaagaaga taaacagcgc attagcgaaa ttctccagga aacgtgggaa
    77701 agcgccgatg actggtttgt cagcgaataa tatgcagtga tttttgtttt tgccgggtcg
    77761 ccccggcttt tttattttac ttacgaattt tgagtacaaa atttctacac ttcctcaaaa
    77821 taattgcaca atatgctaaa aaaatatgaa tgacatgctg ttttattgtt catataaatc
    77881 ttaaaagcag aaatttatct gttcccccct ttaaaaattc ggtgaatacc cttacttatt
    77941 ggtatactga agttgcttca gataaaaacg cattgaacct cgaataacgt tgtctagtaa
    78001 cacgaattag ggggccatga tggaaaaaaa taatgaagtc attcagactc atccgctcgt
    78061 agggtgggac atcagcaccg ttgatagcta tgatgcgctg atgttgcgtt tgcactacca
    78121 gaccccaaat aagtccgagc aggaagggac tgaagttggt caaacgctct ggttaaccac
    78181 tgatgttgcc agacaattta tttcgatatt agaagcagga atcgccaaaa ttgaatccgg
    78241 tgatttccag gtaaacgagt atcggcgtca ttaacatgac atgaccatcc acccaaaagg
    78301 cacctcaccg gtgcctttac catttctggg ttgtatatct gcattcagtt aattactctg
    78361 cttaaaccat gagagcagag agaaaaaaat gaaatacgat ctcatcatta ttggcagcgg
    78421 ttccgtaggc gctgccgccg ggtattatgc aacccgcgcc ggtttaaacg tgctaatgac
    78481 cgacgcccat atgccaccgc atcaacacgg cagccaccac ggcgatacgc gattaattcg
    78541 ccatgcttat ggtgaaggcg aaaagtatgt cccgctggtc ctccgcgcgc aaacgctgtg
    78601 ggatgaactt tcccgccaca acgaagatga tcccattttt gtacgctctg gtgtcattaa
    78661 cctcggcccg gctgactccg catttctcgc caacgtcgcc cacagcgccg aacagtggca
    78721 actcaacgtt gaaaagctcg atgcgcaagg gattatggcc cgctggccag aaatacgcgt
    78781 cccggacaac tacatcggct tatttgagac tgattccggt tttttgcgca gcgaactggc
    78841 gattaaaacc tggatccaac tggcgaagga agcgggctgt gcgcaactgt tcaactgccc
    78901 ggtcaccgca attcgtcatg acgatgatgg cgtaactatt gaaacggttg acggtgagta
    78961 tcaggcgaaa aaagcgattg tctgcgcggg aacatgggta aaagacctgc tcccggagct
    79021 gcctgtccag cccgtacgta aagtatttgc ctggtatcag gccgatggcc gctatagcgt
    79081 gaagaataaa ttcccggcgt ttaccggtga actgcccaat ggcgatcaat attatggttt
    79141 tccggcagaa aacgacgcgt tgaagattgg caaacataac ggaggccagg ttatccattc
    79201 agcggatgaa cgtgttccgt ttgcggaagt ggtcagcgat ggttcggaag ccttcccgtt
    79261 cttgcgcaat gtattgccgg gtatcggttg ctgcctgtac ggcgctgcct gcacctatga
    79321 taattcgcct gacgaagatt ttattatcga taccctaccc ggccacgata atacactgct
    79381 cattaccggc ctgagtgggc acggttttaa atttgcgtca gttttagggg aaatagctgc
    79441 cgattttgcg caagacaaaa aaagcgattt tgatttgacg ccattcaggc tttcccgctt
    79501 ccaataatca tcattacggc ctccagaatg aggccgtttt tacaggagta tttaatgcgt
    79561 cggctgttgc actatctca
//
LOCUS       DAJTZL010000003        58905 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.003, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000003 DAJTZL010000000
VERSION     DAJTZL010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 58905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58905
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.003"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(160..1107)
                     /gene="dusC"
                     /locus_tag="OO544_000170"
     CDS             complement(160..1107)
                     /gene="dusC"
                     /locus_tag="OO544_000170"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HCR6765876.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCIAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIEKL"
     gene            1346..1744
                     /locus_tag="OO544_000171"
     CDS             1346..1744
                     /locus_tag="OO544_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HCR6765877.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            1741..2436
                     /locus_tag="OO544_000172"
     CDS             1741..2436
                     /locus_tag="OO544_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HCR6765878.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            2566..3450
                     /gene="cdd"
                     /locus_tag="OO544_000173"
     CDS             2566..3450
                     /gene="cdd"
                     /locus_tag="OO544_000173"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HCR6765879.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            3600..4319
                     /gene="sanA"
                     /locus_tag="OO544_000174"
     CDS             3600..4319
                     /gene="sanA"
                     /locus_tag="OO544_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HCR6765880.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFRREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            4322..4561
                     /locus_tag="OO544_000175"
     CDS             4322..4561
                     /locus_tag="OO544_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HCR6765881.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            4755..5993
                     /gene="preT"
                     /locus_tag="OO544_000176"
                     /pseudo
     CDS             4755..5993
                     /gene="preT"
                     /locus_tag="OO544_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416651.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
     gene            5987..7222
                     /gene="preA"
                     /locus_tag="OO544_000177"
     CDS             5987..7222
                     /gene="preA"
                     /locus_tag="OO544_000177"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HCR6765882.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRRLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IELGEVKFKKGEKEHPVTL"
     gene            complement(7349..8359)
                     /gene="mglC"
                     /locus_tag="OO544_000178"
     CDS             complement(7349..8359)
                     /gene="mglC"
                     /locus_tag="OO544_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HCR6765883.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(8375..9895)
                     /gene="mglA"
                     /locus_tag="OO544_000179"
     CDS             complement(8375..9895)
                     /gene="mglA"
                     /locus_tag="OO544_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HCR6765884.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            complement(9956..10954)
                     /gene="mglB"
                     /locus_tag="OO544_000180"
     CDS             complement(9956..10954)
                     /gene="mglB"
                     /locus_tag="OO544_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HCR6765885.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            complement(11234..12274)
                     /gene="galS"
                     /locus_tag="OO544_000181"
                     /pseudo
     CDS             complement(11234..12274)
                     /gene="galS"
                     /locus_tag="OO544_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
     gene            complement(12416..13573)
                     /locus_tag="OO544_000182"
     CDS             complement(12416..13573)
                     /locus_tag="OO544_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 family protein"
                     /protein_id="HCR6765886.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRT
                     WTPDASAILYEKYWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVTINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTMLFYHLGLFMHFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGTAISKTSR"
     gene            complement(13590..14258)
                     /gene="folE"
                     /locus_tag="OO544_000183"
     CDS             complement(13590..14258)
                     /gene="folE"
                     /locus_tag="OO544_000183"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HCR6765887.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            14516..15352
                     /gene="yeiG"
                     /locus_tag="OO544_000184"
     CDS             14516..15352
                     /gene="yeiG"
                     /locus_tag="OO544_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HCR6765888.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEKVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSTYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            complement(15384..17375)
                     /gene="cirA"
                     /locus_tag="OO544_000185"
     CDS             complement(15384..17375)
                     /gene="cirA"
                     /locus_tag="OO544_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HCR6765889.1"
                     /translation="MFRLNPFVRVGLCLSAISCVWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTIQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YSVSHNGRWDYGTSELKYYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYLVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGVESSVTVFRNDVKDRISISRTSDVNAAPGYQNFVGFET
                     GANGRRIPVFSYYNVNKARIQGVETELKIPFNDEWKLSINYTYNDGRDVSNGENKPLS
                     DLPFHTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVT
                     KDVKLRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(17667..19136)
                     /gene="lysP"
                     /locus_tag="OO544_000186"
     CDS             complement(17667..19136)
                     /gene="lysP"
                     /locus_tag="OO544_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HCR6765890.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            complement(19341..20222)
                     /locus_tag="OO544_000187"
     CDS             complement(19341..20222)
                     /locus_tag="OO544_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6765891.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            20321..21370
                     /locus_tag="OO544_000188"
     CDS             20321..21370
                     /locus_tag="OO544_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HCR6765892.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATESVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            21444..22301
                     /gene="nfo"
                     /locus_tag="OO544_000189"
     CDS             21444..22301
                     /gene="nfo"
                     /locus_tag="OO544_000189"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HCR6765893.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAVEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKFRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            22305..23393
                     /locus_tag="OO544_000190"
     CDS             22305..23393
                     /locus_tag="OO544_000190"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCR6765894.1"
                     /translation="MNDREKQILKILRRNPLIQQNEIADILQISRSRVAAHIMDLMRK
                     GLIKGKGYILTEQDYCVVVGAINMDIRGMADIRYPQAASHPGSVHCSAGGVGRNIAHN
                     LALLGRDVHLISAIGNDFYGETLLEETRRAGVNVSNCIRLHGHSTATYLAIANKQEET
                     ILAINDTHILQQLTPQLLNTSRDLIRHAGVVLADCNLTPEALEWVFTIADEIPMFVDT
                     VSEFKANKVKNWYSRIHTLKPTQNELEILWGQPIKDDNDRIRAVNSLHQQGVKRIFVY
                     LKDESVFCSDKDGEQFLLTAPAHTTVDSFGADDGFMAGLVYSFLEGYSFRDSARFAVA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(23449..24699)
                     /locus_tag="OO544_000191"
     CDS             complement(23449..24699)
                     /locus_tag="OO544_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCR6765895.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEAAATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTGGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(24799..25740)
                     /gene="rihB"
                     /locus_tag="OO544_000192"
     CDS             complement(24799..25740)
                     /gene="rihB"
                     /locus_tag="OO544_000192"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HCR6765896.1"
                     /translation="MEKRKIILDCDPGHDDAIAIMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRQQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGEMFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            25870..26568
                     /gene="yeiL"
                     /locus_tag="OO544_000193"
     CDS             25870..26568
                     /gene="yeiL"
                     /locus_tag="OO544_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HCR6765897.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFLTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLIKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
     gene            complement(26635..27885)
                     /locus_tag="OO544_000194"
     CDS             complement(26635..27885)
                     /locus_tag="OO544_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCR6765898.1"
                     /translation="MDIMRSVAGMVVLLAIAFVLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSIIEAAANGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFANVSLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTSGTLDVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(27979..28917)
                     /gene="psuG"
                     /locus_tag="OO544_000195"
     CDS             complement(27979..28917)
                     /gene="psuG"
                     /locus_tag="OO544_000195"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HCR6765899.1"
                     /translation="MSELTLSPELLQISAEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSPFEVSIRLDSAREIARA
                     MAVKWQTGLNGGLVVANPIPEQFAMPEETINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLVG"
     gene            complement(28905..29846)
                     /locus_tag="OO544_000196"
     CDS             complement(28905..29846)
                     /locus_tag="OO544_000196"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HCR6765900.1"
                     /translation="MREKDYIVIIGSANIDVAGYSHHPLNYADSNPGKIKFTPGGVGR
                     NIAHNLALLGKNAWLLSAVGGDFYGQSLLAQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMSISDCISAEFLAQHQEFIRGAKVIVADCNLSEEVLVWVLENSGETP
                     VFIDPVSAWKCVKIRDHLSKIHTLKPNRLEAETLSGIALSGREDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDINGESGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFIDSV
                     RFAQGCSSMALACEYTNNPELSIANVRSLVENTECLN"
     gene            complement(30304..31995)
                     /gene="fruA"
                     /locus_tag="OO544_000197"
     CDS             complement(30304..31995)
                     /gene="fruA"
                     /locus_tag="OO544_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HCR6765901.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVAATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILDGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPETQIVEKNA"
     gene            complement(32012..32950)
                     /gene="fruK"
                     /locus_tag="OO544_000198"
     CDS             complement(32012..32950)
                     /gene="fruK"
                     /locus_tag="OO544_000198"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HCR6765902.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTLADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            complement(32950..34080)
                     /gene="fruB"
                     /locus_tag="OO544_000199"
     CDS             complement(32950..34080)
                     /gene="fruB"
                     /locus_tag="OO544_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HCR6765903.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEVGAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            34448..35629
                     /gene="setB"
                     /locus_tag="OO544_000200"
     CDS             34448..35629
                     /gene="setB"
                     /locus_tag="OO544_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HCR6765904.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLADVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPVILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKD
                     V"
     gene            complement(35626..35880)
                     /locus_tag="OO544_000201"
     CDS             complement(35626..35880)
                     /locus_tag="OO544_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HCR6765905.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            36035..36607
                     /gene="yeiP"
                     /locus_tag="OO544_000202"
     CDS             36035..36607
                     /gene="yeiP"
                     /locus_tag="OO544_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HCR6765906.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            36830..38296
                     /locus_tag="OO544_000203"
     CDS             36830..38296
                     /locus_tag="OO544_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCR6765907.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLGMAEKCSPELAGWIKKHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFIAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFVHISDCMQDRVFRHAARTLMLDE
                     QAPTLQIKDVDLTQYADKLIARFANPSLKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            38414..39400
                     /gene="yeiR"
                     /locus_tag="OO544_000204"
     CDS             38414..39400
                     /gene="yeiR"
                     /locus_tag="OO544_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HCR6765908.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYELWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            39439..40152
                     /gene="lpxT"
                     /locus_tag="OO544_000205"
     CDS             39439..40152
                     /gene="lpxT"
                     /locus_tag="OO544_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HCR6765909.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            40564..41130
                     /gene="mepS"
                     /locus_tag="OO544_000206"
     CDS             40564..41130
                     /gene="mepS"
                     /locus_tag="OO544_000206"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HCR6765910.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            41311..42867
                     /locus_tag="OO544_000207"
     CDS             41311..42867
                     /locus_tag="OO544_000207"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HCR6765911.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALNINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            42949..44763
                     /locus_tag="OO544_000208"
     CDS             42949..44763
                     /locus_tag="OO544_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR6765912.1"
                     /translation="MIVRILLLFIALFTFGVQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPSKEDMLSLFSLPIFPEKYWKDHKLSDPLATPPLASGPYRVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNETGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            44764..45858
                     /locus_tag="OO544_000209"
     CDS             44764..45858
                     /locus_tag="OO544_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HCR6765913.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            45858..46883
                     /locus_tag="OO544_000210"
     CDS             45858..46883
                     /locus_tag="OO544_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6765914.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            46885..48474
                     /gene="yejF"
                     /locus_tag="OO544_000211"
     CDS             46885..48474
                     /gene="yejF"
                     /locus_tag="OO544_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HCR6765915.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LITSQGSIVFDGQPLQDLNRRQLLPTRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFAAPQQEYTRQLLALS"
     gene            complement(48478..48822)
                     /locus_tag="OO544_000212"
     CDS             complement(48478..48822)
                     /locus_tag="OO544_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6765916.1"
                     /translation="MTSLQLSIVHRLPQNFRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(49155..50275)
                     /locus_tag="OO544_000213"
                     /pseudo
     CDS             complement(49155..50275)
                     /locus_tag="OO544_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311101.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
     gene            complement(50303..50998)
                     /gene="rsuA"
                     /locus_tag="OO544_000214"
     CDS             complement(50303..50998)
                     /gene="rsuA"
                     /locus_tag="OO544_000214"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HCR6765917.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLVSPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            51148..52908
                     /locus_tag="OO544_000215"
     CDS             51148..52908
                     /locus_tag="OO544_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCR6765918.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NIDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRSDEKALFRDCIYELPLRYMIKHSYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIENFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRPPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            53033..53317
                     /gene="rplY"
                     /locus_tag="OO544_000216"
     CDS             53033..53317
                     /gene="rplY"
                     /locus_tag="OO544_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HCR6765919.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(53456..54463)
                     /gene="yejK"
                     /locus_tag="OO544_000217"
     CDS             complement(53456..54463)
                     /gene="yejK"
                     /locus_tag="OO544_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HCR6765920.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNTKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWEPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            54645..54872
                     /locus_tag="OO544_000218"
     CDS             54645..54872
                     /locus_tag="OO544_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HCR6765921.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            54892..56652
                     /gene="pbgA"
                     /locus_tag="OO544_000219"
     CDS             54892..56652
                     /gene="pbgA"
                     /locus_tag="OO544_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin transport protein PbgA"
                     /protein_id="HCR6765922.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGHYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEDEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            56727..56803
                     /locus_tag="OO544_000220"
     tRNA            56727..56803
                     /locus_tag="OO544_000220"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:56761..56763,aa:Pro,seq:ggg)
     gene            complement(56906..58905)
                     /gene="yejO"
                     /locus_tag="OO544_000221"
                     /pseudo
     CDS             complement(56906..58905)
                     /gene="yejO"
                     /locus_tag="OO544_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
BASE COUNT        14505 a        15367 c        14380 g        14653 t
ORIGIN      
        1 ggcgcgaggt aacatccctg taaaacgcgc cctcagccca cctccatgtg ggctgccccg
       61 gccttcaggg aacgcctcgg caattttgac gccaccaaac aaccgtgcgg cctattgata
      121 aagagctaac acattgtcaa aaaacatcac tatggttttt tagagtttct cgatatcaat
      181 tgcctgaata gcccttgcaa tatcagggga attattcaac acccgaacat gctgaaataa
      241 ttccgttgct tcatcgtatt ctttacgcaa ataactcaac cactgtttaa tccgcgcaac
      301 gtgatataac ccggtatcgc cctgcttttc cagacgggta tatttttgca gcaaagcaac
      361 cacctccggc cacggcattc gcggttcgtt atattttact acccggctca ggttgggaat
      421 attgagcgcc ccgcgaccaa tcatcactgc gtcgcagccg ctgatcgcaa tgcattgttg
      481 cgcgctctgc cagtcccaga tttcgccgtt ggcaatcacc ggaatattca gccgctggcg
      541 aatctcgcca atcgcctgcc agtcaatatg ctccgcgcgg taaccctgct cttttgtccg
      601 tccatgcacc accagctccg tagcaccagc ctgttgaacc gcatcggcga tttcaaattt
      661 cttctcaccg ctgtcccagc ccagacgcac tttcacgctg acgggcaaat gcgccggtac
      721 agcttcacgc atcgcttttg caccctggta gatgagttca ggatctttga gtaacgtcgc
      781 cccgccgccg ctgccgttaa ccgttttcga cgggcagccg caattgagat ccacgcccca
      841 ggaacctaat tccaccgcac gggcggcgtt ctctgccagc cattgtggga actgacctaa
      901 caactgaacg cgcaccagcg taccggatgg tgtccggctg gcgttttgta gctcagggca
      961 aatgcgatga aagactttta ccggcagcag ttgatccacc acgcggacaa actcggtgat
     1021 gcacagatcg tagtcgttaa cttcggtcag caattctcgc accagagagt caagcacacc
     1081 ctccatcgga gccagtaaca cacgcatatc ttcacccgga aaaaaatgag gcgctatgtt
     1141 agcgcctatg gtcaacggat taaaggtctg caaagaggga ttgctgcaaa ctgtaatccc
     1201 tctctatgct tgatgtatgc ctgagttcgt tgcgcattgc gtaatgccgg ggagcgaaaa
     1261 aataagcgct gatttcttaa tgtgatcggt agcacgtttt acgaattaat tgtatgatga
     1321 atccatctca tctggggtgt tgattatgag taagacactg aacattatct ggcaatattt
     1381 acgcgctttc gtcctgattt atgcctgcct gtatgcaggc attttcattg cttccctgct
     1441 accggtaacc attccgggca gcatcatcgg gatgctgatc ctgtttgtcc tgctggcctt
     1501 gcaaattctt ccggcaaaat gggtcaatcc ggggtgctac gtactgattc gctatatggc
     1561 gctattgttt gtgccgattg gcgtaggcgt catgcaatat tttgatttgc tccgcgcaca
     1621 gtttggcccg gtagtggttt cctgtgcagt cagtacgctg gtggtttttc tggtggtgag
     1681 ctggagttca caactggtac acggtgaacg taaagtcgta ggtcagaaag gatcagaaga
     1741 atgatggcga atatctggtg gtcattaccg ctgacgttga ttgtcttttt tgccgcccgc
     1801 aaactggcgg cacggtataa gtttcccctg cttaacccgt tgctggtagc aatggtggtg
     1861 atcattcctt ttttaatgct gaccggcatc tcttacgaca gctactttaa aggtagcgaa
     1921 gtgctaaacg atctgctgca accggcggtc gtcgcgctgg cctatccttt atatgagcag
     1981 ctacaccaga tccgcgcgcg ctggaaatcg atcatcacca tctgttttat cggcagcgtg
     2041 gttgcgatgg tgacggggac ttccgtggca ttgttgatgg gcgcttcacc ggaaatcgcc
     2101 gcgtcaatcc tgccgaaatc agtcaccacg cctattgcaa tggcggttgg cggcagtatt
     2161 ggcggtattc cggcaatcag cgcagtttgc gtgattttcg tcggcatcct cggcgcggta
     2221 tttggccata cattgcttaa tgcgatgcgt attcgtacca aagctgcgcg cggtctggca
     2281 atggggacag cctcgcacgc cctcggtacg gcgcgctgcg ccgagctgga ttatcaggaa
     2341 ggtgcattta gttcgctggc gctggtgtta tgcgggataa ttacttcgct gatcgcaccg
     2401 ttccttttcc cgattattct ggcagtaatg ggctaaaatt tgcgatgcgt cgcgcatttt
     2461 tgatgtaagt ttcacgcgtt gcataattaa tgagattcag atcacatata aagccataac
     2521 gggttcgtaa actgttatcc cattacatga ttatgaggca acgccatgca tccacgtttt
     2581 caaaccgctt ttgcccaact tgcggataac ttgcaatctg cactggaacc tattctggca
     2641 gacaagtact tccccgcttt gttgaccggg gagcaagtct catcgctgaa gagcgcaacg
     2701 gggctggacg aagacgcgct ggcattcgca ctacttccac tggcggcggc ctgtgcgcgt
     2761 acgccattgt cgaattttaa tgttggcgca attgcgcgcg gtgtgagcgg aacctggtat
     2821 ttcggtgcca atatggaatt tattggtgca acaatgcagc aaaccgttca tgccgaacaa
     2881 agcgcgatca gccacgcctg gttgagtggt gaaaaagcgc ttgcagccat caccgttaac
     2941 tatacgcctt gtggtcactg ccgtcagttt atgaatgaac tgaacagcgg tctggatctg
     3001 cgtattcatc tgccgggccg cgaggcacac gcgctgcgtg actatctgcc ggatgccttt
     3061 gggccgaaag atctggagat taaaacgctg ctgatggacg aacaggatca cggctatgcg
     3121 ctgacgggtg atgcgctttc tcaggcagcg attgcggcgg caaaccgttc gcacatgcct
     3181 tacagtaagt cgccaagcgg tgtcgcgctg gaatgtaaag acggtcgtat tttcagcggc
     3241 agctacgctg aaaacgccgc attcaacccg actctgccac cgttgcaggg ggcgttaatt
     3301 ctgttgaatc tcaagggtta tgattacccg gatatccagc gcgcggttct ggcagaaaaa
     3361 gccgatgcgc cgttgattca gtgggatgcc acctccgcaa cgctgaaagc tctcggctgt
     3421 cacagtatcg accgcgtgct tctcgcttaa gcctggtgcc ggatgcggcg tgaacgcctt
     3481 gtccggcttg ccagccctct cctggtgtcg aaattcccgg caaacagttt gccgtttctt
     3541 gcgcaaaacc agcgggtaaa gtagcctgat ggaaattttc cttagatcga gtctcctgca
     3601 tgttaaagcg cgtgttcctc agcctgttag tcctgatcgg cttgctgctg ttgactgtgc
     3661 tcggcctcga tcgctggatg agctggaaaa ccgcgcctta tatctacgac gaattgcagg
     3721 atctccccta ccgccaggtc ggcgtggtgc tcggcacagc aaaatattat cgtactggcg
     3781 taattaatca gtattatcgc taccgcattc aaggagcgat taatgcctat aacagcggta
     3841 aggtaaatta tctattactg agcggcgata acgcattgca aagttataat gagccgatga
     3901 ccatgcgcaa agatttaatc gctgctggtg tcgacccatc agatattgtt ctcgattacg
     3961 caggctttcg tacgctggat tccatcgtgc gtacacgcaa agtgttcgat actaatgatt
     4021 tcattattat cacccaacgt ttccactgtg agcgggcatt atttattgcg ctgcatatgg
     4081 ggattcaggc tcagtgttat gccgtaccgt caccgaaaga tatgctgtca gtacgtattc
     4141 gcgaatttgc cgcccgtttc ggtgcgctgg ctgaccttta tatttttaga cgtgaaccgc
     4201 gttttttagg gccgctggtc cctattccgg ctatgcacca ggtgccggaa gatgcgcagg
     4261 ggtatcccgc cgtcacaccc gaacagttac ttgaattaca aaagaaacaa ggaaagtagt
     4321 tatggatgta cagcagtttt ttgtcgttgc cgtttttttc cttatcccga tattttgttt
     4381 ccgcgaagca tggaaaggct ggcgcgcagg cgcgattgat aaacgggtta aaaatgcacc
     4441 ggaaccggtg tatgtctggc gagcaaaaaa tcccggactc tttttcgctt atatggtggc
     4501 atatatcggc ttcggaattt tatctatcgg catgattgtt tatcttattt tctatcgtta
     4561 atattttctc ttttctgtta attattgaga ataattatta cttcactcaa taagctgcgg
     4621 atataacatt ctgaaccgca gctatttgtg aatcctttca cagtttaaat ttccccacac
     4681 gcttagcctt aatatcagca cgcaatattt atttagcgtt cgccttaatt actcatagca
     4741 ttaaggaaga tcacatgccg caacaaaatt atctggatga actcactccg gcttttacgc
     4801 ctttactggc gattaaagaa gcctctcgct gtttattatg tcatgacgct ccctgtagtc
     4861 aggcttgccc ggcacagacc gatccgggga aatttattcg ctcaatctac tttcgtaatt
     4921 ttaaaggcgc tgccgagaca attcgcgaaa ataatgccct cggtgccgtt tgtgccagag
     4981 tgtgcccgac ggagaaatta tgccaaagcg gttgtacccg tgccggtgtt gatgctccca
     5041 ttgatatcgg ccgcttacag cgttttgtta ctgattttga acaacaaacc gggatggaaa
     5101 tttatcagcc cggtactaaa acgctcggca aagtcgcgat tattggcgca ggtcctgccg
     5161 gattgcaggc cagtgtgaca ctgacaaacc agggttatga cgtcacgatc tatgagaaag
     5221 aagcacaacc cggtggctga ttgcgtaacg gcattccgca attccggtta ccgcaatcag
     5281 tactggatgc agagatcgcc cgtattgaaa aaatgggcgt gaccattaag tgcaacaacg
     5341 aagtcggtaa cacactcacc cttgagcagc tgaaagcaga aaaccgcgcg gtactggtca
     5401 ccgtggggtt atcaagcggt tccgggctac cgctgtttga gcatagtgac gttgagattg
     5461 ccgtcgactt cttgcaacgt gcacgacagg cgcaaggcga tatcagcatt ccacaaagcg
     5521 cattaattat cggcggcggt gatgtcgcga tggacgtagc cagcacgctg aaagttctcg
     5581 gctgtcaggc ggtaacttgc gtagcgcgtg aagagttaga tgagtttccg gcaagcgaaa
     5641 aagagtttac cagcgcccgg gagctgggcg tttcgatcat cgatggattc acgccagtag
     5701 ccgtggaagg caataaagtc acgtttaagc atgtacggtt atcgggcgaa ctgacgatgg
     5761 cggcagataa aattattctc gccgtcggtc agcatgccag actagatgcc tttgcggagt
     5821 tagagccgca gcgtaacacc atcaaaacac aaaattacca gacccgcgac ccgcaagtct
     5881 ttgctgctgg cgatattgtt gagggagaca aaaccgtggt ctatgccgtg aaaaccggga
     5941 aagaagccgc cgaggcgatt catcactatt tagagggagc ttgctcatgt taacgaaaga
     6001 tctttcgatt actttttgcg gcgtgaagtt tcccaacccg ttctgcctct cttcttcgcc
     6061 ggtaggcaac tgctatgaga tgtgtgccaa agcctacgac acaggttggg gcggtgtggt
     6121 gtttaaaacg atcggctttt ttatcgccaa cgaagtctcg ccgcgttttg atcatctggt
     6181 gaaagaagat accggtttta tcggcttcaa aaatatggag cagattgccg agcatccgct
     6241 ggaggagaat ctggccgccc tgcgtcggct gaaagaagat tacccggaca aagtattgat
     6301 cgcttcgatc atgggggaaa atgagcagca atgggaggag ctggcgcgcc tggtgcaaga
     6361 agctggcgcg gatatgatcg agtgtaactt ctcctgtccg caaatgactt ctcatgcgat
     6421 gggtagcgat gtcgggcaaa gcccggagct ggtagaaaaa tattgtcggg cagtgaaacg
     6481 gggttccacg ctgccaatgc tggcgaagat gacgccgaat atcggtgata tgtgcgaagt
     6541 ggcgctggcg gcgaagcgcg gcggcgcaga tggcattgcg gcgattaaca ccgttaaatc
     6601 catcaccaat atcgatctta atcagaaaat cggtatgccg atcgttaacg gaaaatcaag
     6661 tatttccgga tattccggta aagcggtaaa accgatcgcc ctgcgcttca ttcagcaaat
     6721 gcgcacccat ccagaactgc gcgatttccc aatcagcggt atcggcggca ttgaaacctg
     6781 ggaggatgcg gcggagtttt tattgctcgg cgcagcaacg ttacaggtca ccaccggcat
     6841 tatgcagtac ggctatcgca tcgtagaaga tatggcgagc gggttatcgc attatctcgc
     6901 cgatcaggga tttgattcgt tgcaggagat ggtaggtctg gcgaataaca atattgtccc
     6961 ggcggaagat ttagaccgca gttatattgt ctatccccgt atcaatcttg acaaatgtgt
     7021 tggctgtgga cgctgttata tttcctgtta cgacggcggt caccaggcga tggaatggag
     7081 cgagaaaacc cgcacaccgc attgtaatac cgagaaatgt gtgggttgtc tgctttgtgg
     7141 tcacgtctgc ccggtgggtt gtattgagct cggggaagtg aagtttaaga aaggcgagaa
     7201 agaacacccg gtaacgttgt agaacccgcg tcacatccgg catttggttc ctgttgccgg
     7261 atgcggcgtg aacgccttat ccggcctaca aaaccacaca gatcatgatg ttatgtgcgt
     7321 ttatgaccga atgcggacca cattcacatc atttcttacg cgcgtatttc agtgaatcca
     7381 gcgctacggc gaagataata atcgcccctt tgatgatgta ctgccagtat gggtttacgc
     7441 cgatatacgt caggccatag ttgatgacgg taaaaataat caccccggtc accacgccaa
     7501 tcaccgtccc cacaccgccg ctgaacgata caccgcctac cacgcacgcc gcgatagcat
     7561 ccagctcata cataaagccg aggttgttgg tggcagagcc gatacgtccg gcttctaaca
     7621 tcccgccaaa ggcatagaac acgccagaca acgcgtagat catcagcagg ttcaggccga
     7681 cgttgacacc agatactttt gccgcttccg ggttaccgcc aatggcaaaa atgttcttac
     7741 cgaagcgggt tttgttccac aacacccaga cgaacgccac cgcaatcaac gcgtagaagg
     7801 tgatgtaaga gagacggaaa ctccccagcg cgacaaagcc ctgagcaaag gtagagaagc
     7861 cactgtcaaa accagaaatt ggcgacgccc cgacaaagtc gtaatagagc gagttgatgc
     7921 catagacgat gatcatcgtg ccgagcgtgg taatgaacgg cgtcacgttg agataagcga
     7981 taatcagacc gttgatcaaa ccgatcaccg caccaatggc acagacaatc agaataacca
     8041 gcgcaatcgg catcgtcgcc atttccggga acactttgtt ggcgttatcc atggactgca
     8101 ataatgtcgc agccaccact gccgccagcc ctacctgacg accagcagaa agatcggtcc
     8161 cctgggtgac aattaaccct gccacaccga gcgcgataat aatacgcacc gatgactggg
     8221 tgagaatatt acttaagttc aacagactta aaaatgttgg gtcctggaaa ataataatcg
     8281 ccagcaaaac taaaagaacg acgtaaatac cgccctcttt caggtaagta agaaaacttt
     8341 tcttatttaa cgcactcatg ggaagcccct aatcttaaag gtgcaaagac gcaagacgca
     8401 gaatttcgtt ttgcgttgtt gtttttgtat cgacaattcc ggaaacgaga ccattgctca
     8461 tgaccagaat acggtctgtt atccctaaca actcaggcat ttcagaggag ataataataa
     8521 tccccttgcc tttcttcgcc agttcggcaa ttaactgata aatttcaaac ttcgccccga
     8581 catcaatacc gcgcgtcggt tcatcgagca ttaatatttc tggttgcgtt agtagccagc
     8641 gaccaataat caccttttgc tgattaccac cggagagcga accaatttgc gtccgatgac
     8701 ccggcgtttt tacccgcatc gaatcaatca cccactgggt atcgcttttc atccgcgagt
     8761 tatccagtaa accaacttta tttttgtagt tgcgaatatt ggaaattaac gagttaaaac
     8821 caatatccag ataggcataa attcccgttg agcggcgctc ctcagttacc agtgcaaatc
     8881 cgtggtttat ggcttcgttg gcattatggt tattgatctg tttgccgtgc aacgtaatgg
     8941 tgccagccga tttctcgcga ataccaaata acgtctcaac aatatcggta cgtttcgccc
     9001 ccaccagccc ggcaataccg aggatctccc ctttatgcag atcaaacgag acatcgcgaa
     9061 tcgacggctg gcgcagtgac gttaggttac gcacctcgag gatgacttcg cccggcttgt
     9121 tttctttgtc aggaaaacgc tgattaagag aacgcccaac catcatggcg atgatcttgt
     9181 ccatcgtcag tcctgccagc ggttcggtgg cgatccactg accgtcgcgc aatacggtaa
     9241 cttcatcaca taactggaag atttcttcca ttttatgcga gatataaaca ataccgcagc
     9301 cgcgctcttt taatttacga ataatagtga acagatgatt gacctctttt tcggttaacg
     9361 aagaagtcgg ttcatccata atcacaattt tcgcgttata ggaaaacgct ttggcgattt
     9421 cgatcatctg catttgcgaa acggataatg tgccgacgcg cgcacgcgga tcgatatcaa
     9481 tatccagttc atcaaaaatt gctttggttt cgcggtacat tttgtcctga tcgacaaaca
     9541 tgcctttggt gggatatcgc cccagccaca tgttatccat caccgaacgt tgtaatacca
     9601 ggtttaactc ctggtgtacc atcgaaatac cattttccag tgcctctttg gcggaatgga
     9661 aatcgatctc tttaccctgg aataaaatgg tgccggagtc tttttgataa ataccaaaca
     9721 ggcattttaa taatgtcgat tttcctgcac cgttttcccc cattaatgca tggatagaat
     9781 gtggccggac ttttaaatta acgttatcaa gtgccttaac accaggaaag gacttgttga
     9841 taccgctcat ttccaacaag tattccccgg aagacggagt cgttgagctg accatataat
     9901 tttaccttgt tggccataca ataagggcgc agtaaacgac tgcgcccaat cagtcttatt
     9961 tcttgctgaa ttcagccagg ttgtctttat ctacgccaac ataaggtacg cggaccactt
    10021 tgttgtcgat tttccagttg gtgccatcag ccgcaccttt gccatcggcc aggtttttcg
    10081 ccagatcaaa ggtcgctttc gcctggttgt tagcatcgtt cagtactgtg cccgccagtg
    10141 caccggattt caccagcgcc agcgcttctg gcagcgcatc gacgccaaac accggaatgc
    10201 tggacttgtt gtgtgctttc agtgcttcta ccgcgcccat tgccatcgca tcgttgttgg
    10261 cgataaccac ttcgattttg ttggcgttcg ggccagacag ccaggcgtcc atcttatctt
    10321 tcgcctgagc ggtgtcccac attgcggtat ctaactgtaa ctgttcagtt ttgatgcctt
    10381 tatcgttcaa ttctttaatc acgtaagtgg tacgtgcttc tgcatccgga tggcccggtt
    10441 cacctttcag cagtacgaac tgaatctgac cgtctttgtt cagatcccaa ccctgattcg
    10501 ccgcccagtg tttagcaatc aaatcgccct gaataatgcc ggactctttg gagtcagtgc
    10561 caacgtagta ggctttgtcg tagctatcca gcgccttacg agacggttct ttgttgaaga
    10621 aaaccaccgg cacgttttgc ccacgcgctt tctcaatcac cgtacccgca gctgccgggt
    10681 caaccaggtt gattgccagt gccttcaccc ctttcgccag caatacgtcg atctgatcgt
    10741 tctgcttgga ctggtcattc tgagaatcat tcatcagcag ctgaacatct ggcgcggctt
    10801 tcgcatcttg ctcaatagcc ttgcgcacta cagacataaa gttatcgtcg tacttataga
    10861 ttgttacacc aatgcgagta tcagcagcgt gtgcagcggc accgaataac atgctggcca
    10921 tcacagcaga cagggttaac accttcttat tcatggtatc tccggttttt cttatgcagg
    10981 gtagtgcttg agataaatgc tcggcggggc agtagagtta atgaagtgtt actgaacgcc
    11041 gaagctcact tttttaaatt cgttcttcca tgcccggtaa cgctccagaa aacggcttta
    11101 attgttgtta tgacgctatt acctcggtga aagtgattat tcactgttac atacgggtta
    11161 caacgttaaa acggtgcaat catagctatc acattgttaa gatactgtga aatcactcac
    11221 agattgaaag cggttacatc gcctgatttg ttgagttagt gatcgccgcc gcattctggc
    11281 gcgttgcgac agaatggcga cgcactaacg tcggcataaa acagtggctg gcacgaggat
    11341 caatattgcc tgctgccccc tgcaaggcca gttcggtggc taatttagcc attgaagcaa
    11401 tgggataacg cacggtcgtt aattgcgggt cggtgtaacg ggcgatggga atatcatcga
    11461 aaccgatgat tgagagatgt aacggaatcg caatgccatt atcttttaat gctgtcagtg
    11521 cgccagcggc catattgtcg ttataagcaa atatagcggt aagttgtaga ttgcgcccca
    11581 gcagttcaac catcgccgcc tcaccgcccg gcatgtccgg cgtaccagtg ccaatccagc
    11641 tttccggcgg aataatatcc tgctctttca acgcactcat ccagcctgct ttacgcatgg
    11701 cgtcatcttc aatgccgtga ctggaagaaa gataaccaat acgttgatga ccgttattca
    11761 gcaacatgcg cgtcgccatt cgggcaccgc tgagattatc caggcaaacg caacgatggg
    11821 cgtaccccgg cacaacgcgg ttgattaaca ccataccggg aatgttatcc ataaattgcg
    11881 ccagttcatc gtcactcaat gcttttgagt gaacaatcaa cgcattacaa cgctggcgaa
    11941 ttaacacctc aatggcgtga cgctcttttt ccgcttcatg atagctattg ccgattagca
    12001 cgtatttctg atgctgctga gcgactagat ccaccgcttt taccagcgcg ccgaaaaacg
    12061 catcagaaac gtccatcacc accacgccaa tggtgtcgct aacctgagtt gccagcgcct
    12121 gggcattggc gtttggccga taatccagct cactcacggc tttcattact gcttcacgcg
    12181 tgtcggcact gaccagcgtg ctgttattga gcacccggga aaccgttgcc acagagacgc
    12241 cagcctaacg cgctacatca cgaatggtga tcatattcac tacctgttat gagattgcag
    12301 taaatgactg cttgctggcg gctattttgt cagcactgaa tacaggactt cgtgaatcga
    12361 gtcacagcaa tggaaacggt tacagccgtt ttgttaacga atgtgatcca gatcgttatc
    12421 ttgatgtttt agatattgcc gttccggcag cacgcaaagt taactgacgc cagagccatt
    12481 ccaccggccc ctggcggaag taacgcagcc agataacaga gaagaggata ttcgccagcc
    12541 ataccggaat aacaaacgcc agcagctcca ggcggtcaaa atgcataaac aaaccgaggt
    12601 ggtaaaaaag catggtacaa atcagcgttt gcaatagata gttggtcagc gccatccgtc
    12661 cgacgcaggc gatcgcaagc accagcttaa agcggctcaa ttgcggccag aagccataaa
    12721 acagcgacgc atagccaatc gcctgaaacg gcgcactcag ttcccgcggc atttgaagta
    12781 agaaggcaca ccagcgatac gcccagtcca gctgccattg cagggcgatg gcaggaaggt
    12841 taatggtcac cccaatcgcc accagcacaa aaccagtacg acgatagtga cgtaagctga
    12901 actgcccttt cagccagccg ctgcgcatca gcgccgcacc aatgagcatc atccctgcca
    12961 gttgccagcc atactgtgcg cccagtgcca gtaaactgtt gccaacacca tcggcacgat
    13021 tactgatcgc ttcaacgccg ccgtgaagct tccagtattt ttcatacaaa atagccgatg
    13081 catccggcgt ccaggtgcgg ctggtctggc tgtcggaaat cagccccaat aacagcaaaa
    13141 cgccaagccc caccagataa agcatgacgc ctgtattaaa caggcttttt accgatggcg
    13201 catcgcgcac cagccgccag cagattaagc ccaccagccc gtaagccagc agaatatcgc
    13261 cgtcccagaa caataaaccg tgaataaaac ccagcaagac taacagcgtt aaccgcgact
    13321 ggatccagcg tctgccacgg ggcagcaaca tttgcaggcc cgcaccaaac agcagcgcaa
    13381 aaagcgtgag gaatttcacc tggccgatga gatcgagaaa tgcccaggtc catgcatcct
    13441 gcggcgtaat agcgccgtac caggcgggat tgagataagc cgcctttggt agcccaaagg
    13501 cgctgatgtt taatagcagg atccccagaa tggcgacgcc gcgaacaaaa tcgagcgtta
    13561 cgttgcgctc catggttcct gccttttaat cagttgtgat gacgcacagc gcgcagaaac
    13621 tcgtggcgcg tattctgact ggatttgaac aatccaccaa gagaggtcgt tgttgtggca
    13681 ctggttgcat cgcggatgcc acgcgctttc acgcagtaat gcaccgcatc aatggagaca
    13741 gccacgttat tggtgcccag cagcgtttgt agcgcaataa gaatttgctg cgtcagacgt
    13801 tcctgcacct gcggacgctg ggcaaagaac tgcacgatac ggttaatttt tgacagaccg
    13861 atcaccgaat ctttcgggat ataagccacc gtcgctttgc catcgatggt aacaaaatgg
    13921 tgttcacagg tactggtcag agtgatatcg cgcacggtga ccatttcatc gaccttcatt
    13981 ttgttttcaa tgagggtgat tttcgggaag ttggcgtaat ccagaccgga gaaaatttca
    14041 tcgacataca ttttagcgat gcgatgcggc gtttccatca aactgtcatc agccaggtcg
    14101 agattcagca gctgcatgat ttcggtcata tgaccagcaa taaggctttt gcgcgtttcg
    14161 ttatccattt catgcacggg cgggcgcagc ggtgtttcca gtcctcgcgc aactaacgct
    14221 tcatgaacca gggccgcttc tttactgagt gatggcattt atgatttctc ctgcaggtgt
    14281 gacgcctccg ccctgcgtgg gggcaaagtt attaagctga tttacagcct gattattgtg
    14341 cgtgaggcgg cgcacataat ccagtattca cagcgataat tattgtaatt gccgctgact
    14401 ttcatcagca gatgttaaaa catcgttatg caaatacgga agtgaaagtt actcacagca
    14461 cattgaataa acggtatgat gaagaaattg caaacaacac aacaaggagc cacgcatgga
    14521 aatgctcgaa gagcaccgct gttttgaagg ctggcagcaa cgctggcgac acgactccag
    14581 taccttaaac tgcccgatga cgttcagtat ctttctccca ccccctcgtg atcacacccc
    14641 gccaccagtg ctgtactggc tttccggatt aacctgcaat gacgagaact tcaccaccaa
    14701 ggcgggtgcc cagcgcgtgg cggcagaact ggggattgta ctggtgatgc cagacaccag
    14761 cccgcgcggc gaaaaggttg ccaacgacga tggctacgat ttaggccagg gcgcaggctt
    14821 ttatcttaat gccacgcaac cgccgtgggc gacgcattac cggatgtatg attatctgcg
    14881 cgatgaatta ccggcgctgg ttcagtcgca atttaatgtc agcgaccgct gcgccattag
    14941 cggtcactca atgggtggtc acggtgcgct gattatggcg ctgaaaaatc cgggtaaata
    15001 caccagcgtt tcggcctttg cgccaattgt gaatccgtgc agcgtcccgt ggggaatcaa
    15061 agcgtttagc acctatttag gtgaggacaa aaatgcatgg ctggaatggg atagttgcgc
    15121 gctgatgtat gccagtaacg cgcaggatgc gatcccgacg cttatcgatc agggcgataa
    15181 tgatcagttt cttgccgacc agttgcaacc tgcggtattg gcagaagccg cgcgccagaa
    15241 agcgtggccg atgacgctgc gtattcagcc gggatatgat cacagttact acttcatcgc
    15301 ctcttttata gaggatcacc tgcgcttcca tgcgcagtat ttactgaagt gaaagtccgc
    15361 ccggttcgcc gggcatcttc tcatcagaag cgataatcca ctgccataaa gtaacgacgt
    15421 ccgtcttcgt tatagctgta gtcgtcacga ctgagatcct tgtcgccaag gttcagcacg
    15481 cctgcgcgca gtttgacgtc tttagtcacc tgccaggccg cgccggtatt ccagatggta
    15541 taaccgcccg gtgttttagc cgtcgcgctg tcggcgcgtt tctgcccggt atagtgacca
    15601 gaaacataga atgaccagtc ttccagcgcc agcggtttcc agtccagcgt accgttagca
    15661 gtatggaacg gcagatcgga tagcggtttg ttttcgccgt tgctgacatc acgaccatcg
    15721 ttgtaggtgt agttgatcga cagtttccat tcatcgttga acggaatttt cagttcggtt
    15781 tccacgccct gaatacgagc tttgttaacg ttgtagtagc taaataccgg tatgcgccgt
    15841 ccgttagcgc ccgtctcaaa accaacaaag ttttggtagc ccggtgcagc gttgacgtca
    15901 gacgtacggc tgatgctgat acgatctttc acatcgttac ggaaaacggt aacgctggat
    15961 tcaacccctt ccagccagcc ttcttcaccc atgtagtaaa gccccagctc ccaactttcg
    16021 ctggtttctg gtttcagatc cgggctaccc acaatcttac atgcgccacg gcaggaattg
    16081 ctcgtccagt cagggctaag ttgcaacaga gaaggtgctt taaatgccgt cgcccagccc
    16141 cctttcaccg ttacggtgtc ggtggcgtta taaaccaggt aggcacgcgg actccagtgt
    16201 tcaccgtagg tttcgtgatc gtccatacgc acgccggtcg tcagcgccag cggctcgaag
    16261 atccgccatt catcttccac aaacagcgcg tactggctgg cagacgtttt ggagctggtt
    16321 cccccggtca ggttcaccgc atcgctaagt ttgtcgtgac gccattcacc gccaaccgtg
    16381 agaaactgat taatcgccgt cagcggcaac gtgtatttgc cgtcgaccgt attgctttcg
    16441 gaagttatcg ggctgctgtt gccagggttt ttgttctcga ctttctcacc gtagtatttc
    16501 agttcgctgg tgccgtaatc ccaacgccca ttatggctga cggagtagtt ctggcgttcc
    16561 aggcggtttt tgtccagcga gtcggaatca cgatcctgac ggtcgaaacc gtatccggca
    16621 gtaaaatcgt gattttgatt cggtgtccag gcaaattcga cattgccgtc gcggctggag
    16681 aatccttcaa tacgcggcgt ttctccggta tcggtggtcg ttgagttttg cgggtcatcc
    16741 ttttcacgtt ttgccaggct gccgtaagct ttcattccca gcacaccatc aattaatggt
    16801 ccactggtaa agaactgacc gttataggta tcaccgcgat cgcgatgttc ctgaatggtg
    16861 gtatcgacgg taacggtacc cgaccatttc tgaccgattt ttttggtgat gatattcact
    16921 acaccgccga gcgcatcgga accgtacagc gacgacatcg ggccacggac cacttcaata
    16981 cgttcgatgg aatcgaccgg gatccagttc agatcgaaat cattgtggcg gaagacggca
    17041 ttgcgggagt tcacgcgttt accgtcgacg agaatcaggg tatagctgct gtccagacca
    17101 cgaatactaa cgcccttacg gttatcccct tcgttcgtca gttgtacgcc aggcacttct
    17161 ttgaggacat ccttcagatt ctgtaccggt tttcgctgca gatcttcctg ggtaatgacg
    17221 ctgatactgg cgggtgcatc tttaaggttt tgttcaacgg aagatgcagt gacaaccatc
    17281 gtttcgccat cgtcatcgac cgctaacaca ggccatacac aagaaatagc ggacaaacac
    17341 agcccgaccc gtacgaaagg gttcaaccta aacattccat atctccatga ggtaactacg
    17401 aaaataaaat gggtatcgct cacatcttct ttacgtcccc ttgcgtacgg cagcatcgcg
    17461 gtggacttat ttgtatgcag gtgatcatcc cagaaagccg gaccagcctc tgatttgtca
    17521 gccttttttg attgtagcgt aacgataatg caaacgataa caattatcaa tccaaatgtt
    17581 aaattttata tccgcatgat ttgtcaggga aatggaattg aaaaagccct ctcggttgag
    17641 agggcttagc aaggaaggga ggaaacttat ttcttatcgt tctgcgggaa cttcatttcg
    17701 ctgtagcgta cgaagtgagt tcctttaatc agcttgtagc cgaaccaaat aatcaggaac
    17761 agcgggatac caatatacgt tgccgctacg ccgccccagt caatggtatc tttcaggaac
    17821 gcttcgtagt tctggcccaa agtgataatc agacacagaa tgaatgcgaa gatcggcccc
    17881 agtgggaaga aacctgaacg gtacggcaga tcgttaatgt cgtgtccctg caatacgtaa
    17941 ccgcgacgga agcgatagtg gctaatggca atccccagcc aggcgataaa acccgtcatc
    18001 ccggaggtgt tcagcagcca caggtatacc gtctggttgc caaacatgga ggtcaggaag
    18061 cacaaaccgg caatcaccgt cgtcgcatac agcgcattac gcggcacgcc accacgcgac
    18121 aatttagcga aaatgcgcgg cgctttaccg tcacacgcca gggtgtacag catacgagta
    18181 gacgcataca taccggagtt acctgctgac agcaccgccg tcagaataac tgcgttcatc
    18241 accgccgccg cagagagcag acccgcgtgc tggaacacca aggtgaacgg actgacgctg
    18301 atgtctttaa catcgttacg cagcaggctc ggatcggtat acggaataat caggctgata
    18361 atcaggatcg cgaacacata gaacaacagg attcgccaga atacctgacg taccgcgcgt
    18421 ggaatgtttt tcgccggatc ttcggactcg cctgcagcaa taccgatcag ctcggttccc
    18481 tggaaagaga agccgacaat catagctacg ccgatcatcg ccgcaaaacc accagcaaac
    18541 ggcgcttcgc cgattgtcca gttgctccag cccgcaggct gcgcgccttt gaagataccg
    18601 ataatcatca gcacgccaac gatgataaag acaataactg tcgtgacttt gatcagtgaa
    18661 aaccagtatt ccgcttcacc aaagccacga actgagatgt agttcagcag gaagataacg
    18721 ccgaggaaca acgcactcca gatccagccc ggtgtatccg ggaaccacca gctcatgacc
    18781 agctgtgctg caaccaagtc aacggcaata gtcaccgccc agttgtacca gtagttccag
    18841 cccagcgcga agccaaagcc ttcttcaaca tagttctgac cgtaagtggc aaacgaaccg
    18901 gaaaccggca tatatgcagc caattcaccg agactggtca tcaggaagta aaccatcagg
    18961 ccaatcagca tatacgagag caatgccccg cccggacctg cctgagaaat cgttgcgcca
    19021 gaggcaacaa aaagacctgt accgatggaa ccgccaatgg caatcatcgt caggtgacgc
    19081 gcctttaatt cacggcgtaa gcccggcgct tctgtagttt tagtttcgga acccatacaa
    19141 aaatgctatc catcttaaaa atgaggcgcg attgtagcag acgattggca atccttccgg
    19201 cagaaataca cggttataag acaccttcat gatcgcccag ggattataag taaagcagcc
    19261 aatctctttt ctggcgaatg catgctgagg gcgctgcgtc acgaaatcga cacagcacca
    19321 gcatgttctt gtacagcacc ttaacgcggt acattcgcgg gatcgcaata gtccagaaag
    19381 cgccgtagcg cgttggaaag gtgtttttgc cgatgatgta tacgccacaa cgtacgcatc
    19441 aggcgcggga gagggaccgc aacttcactt aatgtgcctg cctgcaattg atcttcaatc
    19501 acacgtcgcg acaggcaact aattcccaac ccatgacgca ccgcatgttt gattgcctcg
    19561 gagttaccta attccatcgc catctcaaac ttcggtaaat gtgacagcaa cagataatcg
    19621 acaatctccc gcgtgccgga accgcgttca cgcaggatcc acggcgcagc ggccagctgt
    19681 tctaaggtga ccggaccacg agccaacggc gaagtcggcg cggcgaaaac caccagctcg
    19741 tcttccagcc acggttcaga aatgatttca gtgctgtggc acggtccttc aataaagcca
    19801 atatcaacgc ggaaatccag caccgcttgc atcacgtcct ggctgttccc aacgctaagt
    19861 tcaatcggca actgcggata atcatggcga taacgggcga taactgcagg cagaatgtag
    19921 ttaccgatgg tactactggc atagatacga atcgcgccgt tgtcttcgcg aaacagttgt
    19981 tcgatttcaa ccgcctgttc aagcaatgcc aacgcacgcg gatagagcag ccgcccgtgt
    20041 tcattaacaa ccagtctttt ccccacgcga tcaaacagtt gcacgccaag ctgcccttcc
    20101 aggtcggtca aggctgcgct cactgctgat tgcgacaacg ccagcatcac cgacgcctgg
    20161 gtggttgatc cacttttcaa tacttctgca aaaacttcca actgccggag ggtgatgtgc
    20221 atagtcgctt accacttata aagattaatt ataaatatat aatcaatttt atttttaaac
    20281 cagttagtcg taaccttatg cccggttaaa ggagagggtt atgacgaata tcaccttaca
    20341 gaaacaacat cgtacactgt ggcattttat tccggggtta gccctgagtg cagttatcac
    20401 cggggtcgcc ctgtggggtg gttccattcc cgcggttgcg ggtgccgggt ttagtgccct
    20461 caccctcgca atcttgttgg ggatggtttt aggcaacacc atctatccgc acatctggaa
    20521 aagctgtgac ggtggtgtgc tgtttgccaa gcaatattta ttacgtctgg gtatcattct
    20581 ttatggcttc cgtctgacgt tctcgcaaat tgccgatgtc ggtatcagtg ggatcatcat
    20641 tgacgtgttg acgctgtcca gtaccttcct gctggcttgc ttcctggggc agaaagtgtt
    20701 tggtctggat aagcacacca gctggttgat cggtgccggt agcagtatct gtggtgctgc
    20761 cgcggtactg gcgactgagt cggtagtgaa agcggaagcc agtaaagtaa ccgtggctgt
    20821 tgcaaccgtt gttatcttcg ggaccgtcgc gattttcctc tacccggcga tatatccgct
    20881 gatgtctcaa tggtttagtc cggaaacttt cggtatctac attggttcta ctgtgcacga
    20941 agtggcgcag gtggtggcgg caggtcatgc catcagcccg gatgcggaaa acgcagcagt
    21001 tatttccaaa atgctgcgcg tgatgatgct ggctccgttc ctcatcctgc tggcggcgcg
    21061 tgttaaacag ctgtctgggg cgaacagcgg cgagaaaagc aaaatcacta ttccgtggtt
    21121 tgccatcttg ttcatcgtcg ttgccatctt taactcgttc cacctgttac cgcagagcgt
    21181 ggtgaacatg ctggtaacgc tggatacctt cctgctggca atggcgatgg cggcgctggg
    21241 tctgaccact cacgtcagcg cgctgaaaaa agctggggcg aaacctctgc tgatggcact
    21301 ggtactgttt gcctggctga ttgttggtgg tggtgcgatt aactatgtga ttcaaagcgt
    21361 catcgcataa accactacat cttgctcctg ttaacccgct atcattaccg ttttcctcca
    21421 gcgggtttaa caggagtcct cgcatgaaat acattggagc gcacgttagt gctgctggcg
    21481 gtctggcaaa tgccgcaatt cgcgccgtcg aaatcgacgc aaccgcgttt gccttgttca
    21541 ccaaaaacca acgtcagtgg cgtgccgcac cgctcacgac gcaaaccatc gatgaattca
    21601 aagccgcctg tgaaaaatat cactacacat cggcgcaaat tcttccccac gacagttatc
    21661 tgattaacct cggacatccg gtcactgaag ctctggaaaa atcgcgcgat gcctttatag
    21721 atgaaatgca gcgttgcgaa caactggggc tttctttgct caacttccac cctggcagcc
    21781 atctgatgca gatttcagaa gaggattgcc ttgcgcgtat tgccgaatcc atcaacattg
    21841 cgctggataa aactcaaggt gtaacagcgg tgattgaaaa caccgccggt cagggcagta
    21901 acttagggtt taaattcgaa catctcgcgg cgattatcga cggcgtggaa gataaattcc
    21961 gcgtcggcgt ctgcattgat acctgccatg ctttcgctgc cgggtatgat ttgcgtactc
    22021 cagccgaatg cgaaaaaacg ttcgctgatt ttgcccgtat tgtcggcttt aagtatctgc
    22081 gcgggatgca ccttaacgat gcgaaaagta cctttggcag ccgcgttgac cgccatcata
    22141 gcctcggtga aggcaatatc ggtcatgatg cgttccgctg gatcatgcaa gacgaccgtt
    22201 tcgacggcat tccgctgatc cttgaaacca tcaacccgga tatctgggca gaagagatcg
    22261 cctggctgaa agcgcaacaa actgaaaaag cggtggcctg atcgatgaac gacagggaaa
    22321 agcagattct taaaatttta cggcgtaacc cgctgattca acagaatgaa atcgccgata
    22381 ttctgcaaat cagccgctca cgtgttgccg ctcatattat ggatttgatg cgcaagggtt
    22441 tgattaaagg caaaggatat atccttaccg agcaggatta ctgtgtggtt gtgggtgcaa
    22501 tcaatatgga tattcgcgga atggcggata tccgttaccc gcaagcagcc tctcatccag
    22561 gttctgttca ctgttccgca ggcggcgtcg gacgtaatat cgcgcataac ctggctctgc
    22621 ttggtcggga tgtgcattta atatcagcta ttggcaacga tttttacggc gagacattac
    22681 tggaagaaac gcgccgcgcg ggcgtgaacg tttcaaactg cattcgtctg catggacaca
    22741 gcactgcaac ttatctcgct atcgctaata agcaggaaga aactatcctg gcaatcaatg
    22801 acacacatat tctgcaacag ctaacgcctc aactgctcaa tacttcacgg gacttgattc
    22861 gccatgctgg tgtggtgctg gctgattgta atctcacacc tgaagccctg gagtgggtct
    22921 ttaccattgc tgatgaaatt ccgatgtttg tcgataccgt ttccgaattt aaagcgaaca
    22981 aagtaaaaaa ctggtacagc cggatccaca ctctgaaacc aacacaaaat gaactggaaa
    23041 ttttgtgggg gcaaccgatt aaagacgaca acgatcgcat cagagctgta aattcgctgc
    23101 atcaacaagg tgttaaacgc atttttgttt atctgaaaga tgaatctgta ttttgcagcg
    23161 ataaggatgg agaacaattt ttgctgaccg cgccagcgca tacgacagta gacagttttg
    23221 gtgctgacga tggttttatg gcgggcctgg tatatagctt tcttgaagga tacagtttcc
    23281 gcgacagcgc ccgttttgcg gtagcctgcg cggcaatttc gcgcgccagc ggcagcttaa
    23341 acaaccctac cctgtctgcc gataacgcgc tttcattagt gccaatggtg taacaatgtt
    23401 gccggattcg acgcttgacc gcatcttatc cggctcataa attgcgcact acgctaaacc
    23461 aataaagaat cctgcaatag tagcactcat caggttagaa agtgtcgccg ccgccagcgc
    23521 gcgtaaacca agttgggcga tttccggcgc acggtgtggc gcaaccgcag aaaacgcccc
    23581 caccaccacc ccgatagaac caaagttagc gaaaccgcac aacgcgaaag aaataatcgc
    23641 cacggtttta gcatccagag tgccacccgt ttgcagatat ggcgagaaat tgagataagc
    23701 gacaaattca ttgatcgcca gcttctgccc aatcaaactc ccggcaagat ttgcatcact
    23761 ccagtcaacc cccatcaccc acgccaatgg ggccaacagg taacctaaaa tcgactccag
    23821 cgaagcatgt tcaaaaccaa accagccgcc aacaccgccg ataataccgt taatcaacgc
    23881 aattattgca acaaatgcca taacaactgt cgctacaccg gcggcgattt tcagcccggt
    23941 cattgcccct gtcgcagcgg cttcaataat gctttttggc ggtgtttcgg tgaaagagag
    24001 gttattaaaa gaaacctgcg aagattccgt agccgggctt aacaggcggg caaacaagat
    24061 cccgccaggg atcgccatta acgatgccgc cagcaaatat tccacaggta cgcccagtgc
    24121 ggcgtaacca atcattgtcg aaccagcaat cgaggccatg ccactacaaa tcgctgtaaa
    24181 taattcattg cgattcagac gatcgataaa gggtttgacg atcgccggaa tttcgttttg
    24241 cccgaggaaa atggtggtga ccgcgacgaa tgactcgatc ttgctgatat ttaaagcttt
    24301 ctggaagata ccgccgagaa tgcgaattaa gatccccatc acaccgatgt agtagagaat
    24361 actcaccagt gcagtgacga agataattgc cggtaatacc ctgaaaccaa agataaatcc
    24421 tgcgccatca aataaggtat ccattttcgg tccgaccaga gaaccgaaga taaatgcgct
    24481 acctgcgtcg ctgtacgcca tcactttgtg gacgccaaaa gcgacttttt ctgcgaccca
    24541 acgccctggc ggtaaccaaa gcataatccc gccaatcacg acctgtaaca ctaacgccgc
    24601 gccaacggta cgcaggctga tcttcttctt gtttactgac agtaaaaacg caattgccag
    24661 cagtaccacc attcccagaa cacttctcat gacatccata gtgattatct cttcatgtca
    24721 ttttgggcga cggctttctg cccatcagaa atagccgggc ctgcatcttg cgatactgtc
    24781 ccggcttgtt atcaacactt aatgggtttt tatgtagccg cgtacgcact cttcgactaa
    24841 tccccagaac cagtcggtat caatagtgat gccgacttta gtattggcgg gcttgccaag
    24901 aacgcccagc tcgtcgcaga cggtacgccc atagcaaggg ccactgttga cgtccacttc
    24961 gacgtacatc tcctgggttt taatgccatc agggttaatc agataaccga tgcaggtggc
    25021 gtcgtgcacc gggccgccag ccaggccgta gttttcgaac tgcgttttga gagtgaagtt
    25081 catgatgtcg ctgaacatct ctccggcagg gccgcctgcc ctttccattc gtgcaattac
    25141 gtccggggtg caaacggtct ggttggtaag atcgaggccc atcatcacta atggaacgcc
    25201 ggaggtgaac accacgcgtg cggcttccgg gtcggcaaag atattgaact cggcagatgg
    25261 ggtgaagttg cctgtaccgt aagcgccgcc cattagcaca atttcacgga ttttgggcag
    25321 gattgcgggt tgcatacgca ttgccaccgc gatatttgaa agcggaccaa ccggcaccag
    25381 agtgatatcg ccatcgcttg ccataagggt atcaatgatg tatttcaccg cgtgggtact
    25441 ttctgcctgg cgggtcagcg gctcgaatac cgggccatcc agtccggttt caccgtgaat
    25501 attatcggca acgatttgtt gacgcataat gggttgcggc atccccgcat aaaccggaac
    25561 attaatctcc agtttctggc aaacattcag gccattaatt aatgttttat caagcgtctg
    25621 attacccgca acaatggtga tacctaataa atcaatcgcc ggatgtttcg ccgccatcat
    25681 tatagcaata gcatcatcat gacccggatc acaatccaga ataatttttc tcttttccat
    25741 tgtttatttc ctctgtttcc agttgcgtta ttttttctac agcaacgaaa tttcgatcgg
    25801 gccgatgatt gaatcttaac aacagcgtac gaatgctaaa tatgagaaat ctcatagcgg
    25861 ataaacatcg tgaaagaaat ccacaataat gatcttaagc agcaattgat gagtgaatcc
    25921 gcgtttaagg attgcttttt aacggatgtt tcagccgata cgcggctgtt tcatttttta
    25981 gcgcgtgact acattgtgca ggaagggcaa cagccgtcct ggctgtttta cctgacgcga
    26041 ggccgcgcca ggctttacgc cacgctagct aatggtcgcg tgtcgctgat cgatttcttt
    26101 gccgccccct gttttattgg cgagattgag ttaatcgaca aagaccatga accgcgtgcg
    26161 gtgcaggcta ttgaagagtg ttggtgcctt gcgctcccta tgaaacatta ccgcccgctg
    26221 ttattaaacg acacgctatt tttacgaaaa ctctgcgtca ccttaagtca taaaaattat
    26281 cgtaatattg tttctttaac tcagaatcaa tcatttccgt tagtgaatcg cctggcagca
    26341 tttatattac tctcgcagga aggtgatctt tatcacgaaa agcatacgca agcggcagag
    26401 tatttaggcg tttcttatcg acatctttta tatgttctcg cgcaattcat tcacgacggt
    26461 ttattaatta aaagcaagaa agggtatcta attaaaaaca gaaagcagtt gtcaggactg
    26521 gcgctggaga tggacccgga gaataaattc tccgggatga tgcagtaaaa attattggca
    26581 atagcacgat tgccggatgc aacgcttaat aaggcgtaca tccggcaatg aaactcaagc
    26641 taaaccaata aagaacccgg caatggtcgc gctcatcagg ttggaaagcg tcgccgccgc
    26701 cagcgcccgt aaaccaagct gggcgatttc cggcgcgcgt tttggcgaaa tagccgaaaa
    26761 cgctccaacg acaacaccga tagaaccaaa gtttgcaaac ccacagaggg cgaacgagat
    26821 aatggcaatg gtcttgacat ccagcgtgcc gctcgtttgc agatagggcg agaaattgag
    26881 ataagcaaca aactcattaa tcgccagttt ctgcccaatc aaacttccgg caagatttgc
    26941 atcactccag tccaccccca taatccatgc caacggggcc agcacatagc cgaaaatgct
    27001 ttccagagag acattggcaa aaccaaacca gccgccaact ccaccaataa tgccgttgat
    27061 cagcgcaatg atcgctacaa atgccattac caccgtcgcc acaccggcgg cgattttcag
    27121 cccggtcatt gcaccgttcg ccgcagcttc aataatgctt tttggcggtg tttcgctaaa
    27181 cgacagattt tcaaacgtaa cttgcgaagg ttccgtcgcc ggactaagaa tacgtgcaaa
    27241 caggataccg ccaggaatag ccatcagcga agcggctaac aaatagtcaa tcggcacccc
    27301 cattccggca tagccaatca tcatcgaacc cgcaatagac gccatgccgc tacaaatagc
    27361 cgtaaatagc tcgttgcgat tcatgcgatc aataaaaggt ttaacgatcg ccgggatctc
    27421 attttgcccg aggaaaatag tggtgactgc gacaaaagat tcgattttgc tgatgttgag
    27481 ggctttctga aaaatactgc caagaatacg aatcagcagc cccattacgc caatgtagta
    27541 cagcaagctg ataagcgcgg taacgaaaat aatcgccggg agtacgcgaa aggcgaagat
    27601 aaagcccgca ccgtcaaaca gaacatccat tttgggaccg accagcgaac caaaaataaa
    27661 ggcacttcct gcgtcactgt aggacatcac tttatggacg cctaatgccg cctgctctac
    27721 tgcccatttt cccgggggaa aatagagcat gatgccacca atggcaattt gcagcagcag
    27781 cgcagcgcca accgtgcgca agctgatgct ctttttattt accgacaaca caaaggcaat
    27841 tgccagcaat accaccatcc ccgcaacact tctcattata tccataatga ttttcccttc
    27901 atgccagtaa acccggcgtc agcgccaggt tttggtatgt ttgatgagta cgggcgacgg
    27961 ctttctgccc gccagaaatt aacccacgag acgctgatat tctttggcaa tttcgctcgc
    28021 cagaatggcg ttattaaaga ccagttgaat attggatttc aggctatcac cgccggttag
    28081 ctccgcaaca cgcgccagca ggaacggtgt actctctttc ccaataacac cctgttcttc
    28141 ggcttcagca acggcttgat caattgctgc attgatggtt tcttctggca tcgcaaactg
    28201 ttccgggatc gggttcgcta ccaccaggcc gccgttcaaa ccggtttgcc acttcactgc
    28261 catcgcgcga gcaatttcgc gggcactgtc aagacgaatg ctgacttcaa atgggctggt
    28321 tcggcagaaa aacgcaggca gcgcttttgt ctgataacca attaacggta caccgaaggt
    28381 ttctaaatat tctgttgtta atcctaaatc gagaatagat tttgcccccg cacaaacaac
    28441 agtgacattg gtatttgcca gttcctgtaa atcggcagaa atatcgaagg tatgttccgc
    28501 tccgcgatgc acaccaccaa tacctccggt tgcaaaaact ttaatcccag ccagtgctgc
    28561 aataatcatg gttgatgcaa cggtagtcgc gccatttttc cctgcagcaa cgacaaaagg
    28621 taaatcacga cgactaactt tcgtcacgtt atgtccttca cggcccagca attcgatttc
    28681 ttctttactt aagcccactt tcatcacacc gccaataatg gcgatagtgg ctggtactgc
    28741 gccctgttta cgaattgttt cttctacttc aattgcggtt tgggcatttt gtgggaatgg
    28801 cattccgtga gaaattatgg tcgattccag cgcaacaacc ggttttttat tttttaaagc
    28861 atcctgtact tcagcggata tttgtaataa ttcaggggac agagttaatt cagacattct
    28921 gtgttctcca ctaacgatct aacattggca attgataatt cagggttgtt ggtatattca
    28981 caggcaagtg ccattgacga acatccctgc gcaaatcgaa cagaatcgat aaacggcata
    29041 ccgtctaccc aacaggaggc cagccccgcc atcatggcgt cgcctgcccc agtaacattg
    29101 atgacattcg ttttaatagg ggccgaccac ccactttcac cgttgatatc actgtagtaa
    29161 acgccatccc ctcccatgct cagcaccaag cggttcaggc cgtgttgatg aaaccaggcg
    29221 gcgacctttg cgacatcttc acgtccggaa agtgcaattc cgctcaacgt ttcggcttca
    29281 agacggttag gttttaacgt atggattttg ctcagatgat cgcggatttt gacgcatttc
    29341 catgccgata ccggatcaat aaatacaggc gtttccccgc tattttccag aacccagacc
    29401 agcacctctt cactaaggtt gcaatccgcg acaataacct ttgcaccgcg aataaactcc
    29461 tgatgctggg caagaaattc tgcagaaata cagtcactaa tactcatatc atttatcgcg
    29521 acaagcattt ctcctgtatt atcgagcagt gataaatagc tggatgtatt ctcacccgga
    29581 acaattaagc atttatcgac ataaacaccg gattgattag tttgcgccag taatgattgc
    29641 ccataaaaat caccacctac tgcacttaaa agccaggcat ttttccccag taacgccaga
    29701 ttatgagcga tattccttcc cacgccacct ggggtaaatt taatttttcc gggattcgaa
    29761 tccgcataat tcaatggatg atgtgaatac ccagccacat caatattcgc cgaaccaata
    29821 attactatgt agtccttttc gcgcatagca cgccccctgg caataaattg ccaacgaaga
    29881 aattgggtaa aatagccatt acagtcaata agactattag agcacgtgtt cagatttaaa
    29941 cacatgttca taataagcat atttaacgta atgtaaatcg atcagaaaaa acgaaatcgg
    30001 ccagatatga aggcggtcac aaagtttgtc tatttatgca gcaacaaaag agaaagttgt
    30061 gatgtgtaac acacaatatg gcaggcaaga aaaagggcag agatgtttct gccctccaga
    30121 ttgttgacaa cgcgcgcttt gttcatcctg gatgtggcgt aaacgcctta tccgacctac
    30181 aaaagcgcaa aaaattcaat atattgcagg gttcatgtag gtctgataag catagcgcat
    30241 caggcaattt tgcgtttgtc atcatcgggg cagagatgtc tcatccccga taatgaagat
    30301 aaattatgcg tttttttcta ctatttgtgt ttccgggcgt ttcaggaagg cataggccaa
    30361 acccgccacc agcgtaccgg caataattgc taccaggtaa cccaataccg gcgtaatagc
    30421 gccagggatc agcagaacaa acagaccacc gtgtggtgcc atcagtttcg caccaatcgc
    30481 cattgagatt gcgccagtca gcgccccacc aacgatacag cacggtagca cacgcatcgg
    30541 atcacgagca gcaaacggaa ttgcaccttc cgaaataaag cacagaccaa gtaccagagc
    30601 ggctttgcca ccttcctgct gcgctttgtc gaatttgcga cgcgccacca ttgttgccag
    30661 acccattgcc agcggtggca ccatacctgc cgccataatc gccgccatcg ggccataggt
    30721 ttgagtactc agcagaccca caccgaatgc gtacgctgct ttgtttaccg gaccgcccat
    30781 gtcagtacac atcatgccac cgaggatcgc ccccagcaga accgcattcg cagtccccat
    30841 ggtctgcagc cagtgagtca acccatcgag aatgccagca accggtttac cgattaggta
    30901 gatcatcgcc agaccgacca ccagactgga aattagcgga atgatcagga tcggtttcag
    30961 cgcctccata ctctgtggca gtttcaattg cgtactgatt aacttcgcaa tgtaaccagc
    31021 caggaagcca gcaattatac cgccaataaa gccagaaccg gtgctgaccg ccagcatacc
    31081 gccaatcaga cccggtgtga ggcccggacg atcggcaatg gagaaggcaa tataacctgc
    31141 cagtaccggc accatcagcg caaaggctga accaccacca atctgcatca gcgccgctgc
    31201 caacgtaccc ggctctttaa acgcttcgat accaaaagca aaagaaagcg cgatacacag
    31261 accacctgca acaaccatcg gcagcatgta agagacgccc gtcagcaagt gacggtaagc
    31321 gcctgcactc tctttcttac cttcagtggt cgctgtttga gctttgcccg ctggttcata
    31381 cggcgttgct tcagcaaccg ctttatccag ttcctgcgca gttttcttca gcgccagacc
    31441 ggtagaggtg cgatacatcg gtttaccagc aaatttcgcc agatccactt cgatatctgc
    31501 cgccacaatc accagatccg ctgctgcgac ttcttcaggg gtgattgcat tgcccgcgcc
    31561 aacagaacca cgggtttcaa ctttcaccca ccagccacgt tttttcgctt cggtttcaat
    31621 ggcttcagcc gccataaagg tgtgtgctac gccagtcggg caagcagtca ccgcaactac
    31681 gcgtttcgga ccgctggcag caaccggtgc tgtcgcagcg accggcgcag tgtaaggttt
    31741 cgcatggcct ttggcttcac tcaggaacag ctcagggtgc gcaactgccc gggaaatatc
    31801 acccagccag acatttttac cgttcagcgc gctgtcattc gggatggaat caccgagaac
    31861 aatcgccatt tctgcgtcgt tcggattgtc gatgatttcc agttttgctt ttcgcgccgc
    31921 cgcgcccagc agggtcttcg ccatataggc gcgtgcctga ccgagattag cgtcaataat
    31981 cagcagcgtt ttcattatgc ctctcctgct gtcagttaaa aggttgtaag tcgacgcgcg
    32041 ccatcattgc ggccaactgc ggacgatcgg taatacccac attgctttga cttaccgcca
    32101 gggctgcaac agctgtcgcc agacgcagtg tgtgttcact ggattcacgc atcagcaagc
    32161 cataaatcag gccaccaacc atagaatccc ctgcgccaac ggtgcttacg acatcgactg
    32221 acggtggttt ggcgatccat tcgccggagg cattaaccca aagcgcgcct tcggcaccca
    32281 gtgaaataac aacatgcgca ataccttgtt cacgtagcgc atgtgcagct tcaatcacat
    32341 ctttcatttc aggcagttta cggcctgccc agatttccaa ctcgcggcgg ttaggtttca
    32401 ccagccacgg tgccgctttc aaacctgcta ctaacgcttc acggctacta tcaaagataa
    32461 tgcaaggaca ctgactacgc aggcgagtca tccagtcggt gaacgcttcc gggctgacgc
    32521 ctgacggtaa gcttccgctg acacagacca tatcgaactg accgagccag ctcagagaat
    32581 cagtcacaaa gcgttcccag tcggcgaggg tgacttcaaa acccgagaag ttgaagtcgg
    32641 tcacttcgcc gtctttttcc gtcagcttaa cgttaattcg ggtgcgcccc tgtacaacct
    32701 ggaaacggtt ggcaatgccc agctcgctga acagttgctg aaaaccatcc tgattgtctt
    32761 tacccaggaa gccgccaacg gtgacatcaa ttcccaggtc ttttaatact ttggccacgt
    32821 tgatgccttt acccgccgca tgcagaccgg tggttttcac caggttcact tcgccgcgtt
    32881 caatttccgg gcagaaacca acaaggtcat aagccggatt aagggtgatc gtagcaacac
    32941 gtctgctcat tatgcgccct ccccaagacc agcagcgata gcgtcgccaa tggctttcag
    33001 cgcctgttca gcatctgcac cctgggcggt aaagcgtagg cgatgacctt tcttaacgcc
    33061 aagtgccaca actttcatca gactacgtcc gtttgccggt ttgccggtgc catcaaggtt
    33121 tgtcacggta atatcactgt taaattgttt aatggtattg accagcatgg tacctggacg
    33181 agcatgcagg ccgtgttcat tgcgcaccac aaactccgcg cttaacacgt cgtcggtcgg
    33241 cgcatcatcg ctggtcagca gcgccagcaa cgttgccgca tccgctttca gcaagcggtc
    33301 agctttattg tcgagcaata aatcagcgag acgcttaaga accgcgatgg gctgatcgtc
    33361 attcatcgcc acactcacca gcatggctgc cgtttcgccg tccacatcaa aagcatttgc
    33421 cgcacggctt accgcaatcg cgctacgcag attgccttcg gcgctatcgc tcagccagat
    33481 accctgtccg agattcagcg gttgttcatt gatggctttg gtgacgaaag tggcgtcaac
    33541 tgcccccacc tctttcagac gcgcagcgtt cagcgcctga agagtcagca gatcgctggc
    33601 gacgatatcc agcgtcagca tttcgttgtc gagcttcagc tgctcactct gcttttcgcc
    33661 catcagtaat gcgcgaagtt cttctgctgt tgttgctgac ttcagttgtt cagcaacgga
    33721 atcatcgctc agtacgtggg tcagctggcg tagcaggccc agatgttcat ccgagctggc
    33781 agcaataccg attgccacgt acgctacctg accgtcaccc caggtgacgc cttccgggaa
    33841 ctgaaatacc tgaacgccgg ttttcagcac ctgatcgcgg gtgtcggtag tgccgtgtgg
    33901 aatagcaata ccattgccga ggaacgttga ggtttgctgt tcgcgcgcca gcatgccatt
    33961 gacgtagcct tctgctacat taccggcctg caccagcgcc gcagcgacct ggcgaatcgc
    34021 ctcttctttg tctccggcct tttcgcccgg atggatgtcc tgtacggata actggaacat
    34081 agttctcctc tcttgctgaa ttgaaacgat tcagcctcta tgagaaaaaa agcgccaacc
    34141 tggcttaggg ttaaagacaa gatcgcgctg aaacgtttca agaaagcata atacttctgt
    34201 ttcagcacgc aaggaaagcc gtaaagttat gagcaaaagt ttgatgtgct gcacaatttt
    34261 ttgtcatttt tcctaattgc tgatgggaaa atccgttatc agcaatttca tttcagcagc
    34321 gtcagcagcc aatcagcaac agtgaaatgg cattttgatt attcgtggcg cttttgattt
    34381 cgctgtctgt ttattttctg acaagcagcg taaactccgc gtcttcctct tccagtgatc
    34441 gacctgcatg cataactccc ccgcagtctc cagcgcgaaa tcgtttgacc tgacctcgac
    34501 ggcgttttta atcgttgcct ttctcaccgg tattgcgggt gctctgcaaa ccccgacact
    34561 cagtattttc cttaccgatg aagtacatgc ccgtccggcg atggtgggat tcttctttac
    34621 cggcagcgct gtcattggga ttctggtaag tcagtttctc gctgggcgct ctgataagcg
    34681 cggcgatcgc aaatcgctga ttgtcttttg ctgcctgtta ggcgtgctgg cctgcaccct
    34741 ttttgcctgg aatcgcaact actttgtttt gctattcgtt ggcgtctttc ttagcagctt
    34801 tggctcgacc gctaacccgc aaatgtttgc ccttgcccgt gaacatgccg acaaaaccgg
    34861 acgtgaggcg gtgatgttca gctctttttt acgcgctcag gtttcactgg catgggtcat
    34921 tggcccaccg ctggcttatg cgttagcgat gggtttcagc tttacggtaa tgtatctgag
    34981 cgcagcggta gcgtttattg tttgcggcgt gatggtgtgg ctgtttttac cgtcgatgca
    35041 aaaagagctt ccgctggcga ccggcacggt tgaagcgccg cgccgtaacc gtcgcgatac
    35101 gctactgctg tttgtcattt gtacattgat gtggggctcg aacagcctgt acatcatcaa
    35161 catgccgcta tttattatca acgaactgca tcttcccgag aaactggccg atgtgatgat
    35221 ggggaccgcc gccgggctgg aaatcccgac gatgttgatt gccggatatt tcgccaaacg
    35281 tctgggtaag cgtttcttaa tgcgcgttgc tgccgtgggt ggcgtctgtt tttacgcagg
    35341 aatgctgatg gcgcattcac ctgtcattct gttgggcttg cagctgctaa atgctatttt
    35401 tattggcatt ctgggcggca tcgggatgct ctattttcag gatctgatgc ccggtcaggc
    35461 gggttcagcc accacgctct ataccaacac ttcgcgcgtg ggctggatca tcgcaggatc
    35521 agtggcgggc atcgtcgccg aaatctggaa ttatcacgct gtgttctggt ttgcgatggt
    35581 gatgattatc gccactctgt tttgcttact gcggattaaa gatgtttaag gcgcggtcag
    35641 catttccaga tcgataagcc aggtcatggc ctgctgacgc gagttaccgc acatttcagc
    35701 gctggcttgt aagcctgcac agactttcgg tcgcagtggc gaggtgaaaa ttttacagcg
    35761 ttgctgttca tcaagctgaa tgcagggcgt attggcgggc ttgccatctg gcataccggg
    35821 aatagggctg gaaattgaag gggcagtgca acacgccccg caacccggac ggcattccat
    35881 acgttgttct cttaaggtta accaccggat tgatgagcgc gcacagtacc accttttttg
    35941 caccagcaaa agtgcgaata ccacttgccc gaaaggcccg tcgcgagtac tttgtcgcga
    36001 tatttttgac attttcgact acaggaattt ttcgatgcca agagcgaacg aaattaaaaa
    36061 aggtatggta ctgaattaca acggcaaact gctgctggtt aaggatattg atattcagtc
    36121 gcccactgcc cgcggtgccg ctacgctgta caaaatgcgt ttttctgatg tccgtaccgg
    36181 gctgaaagta gaagagcgtt tcaaaggtga tgatatcgtt gacaccgtga cgctgacccg
    36241 ccgttacgtt gacttctcct atgtcgatgg caacgaatat gtctttatgg ataaagaaga
    36301 ctataccccg tataccttca ccaaagatca gattgaagaa gagttgctgt ttatgccaga
    36361 gggcggcatg ccggacatgc aggtgctgac ctgggacggt caactgctgg cgcttgagct
    36421 gccgcagacc gttgatctgg aaatcgttga aaccgcacca ggcatcaaag gggcatctgc
    36481 cagcgcccgt aacaaaccgg cgacattgag cactggtctg gtgattcagg taccagaata
    36541 cttaagcccg ggcgaaaaaa ttcgtatcca tatcgaagaa cgccgttata tggggcgtgc
    36601 tgactaactt cagccgcatg cagaaaaggg atagctcagg ctgtcccttt tttaatttat
    36661 tataccaatc ttctattttg cgcttcctga acaccttacg ccctcaattt tcactcgttg
    36721 attgatcgcc ctcacacttc atcgcattaa caatccagac caatttcaat tgctgtcata
    36781 taactttaca ctgtcattgt taatttatcg ttactaagac gtgactccta tgaagacaat
    36841 tgcctccgtt acgctcccgc atcatgtaca cgctccacgc tacgatcgcc agcagttgca
    36901 atcacgtatc gttcattttg gctttggcgc ctttcaccgc gctcatcagg cgttactgac
    36961 cgatcgtgtg ctgaatgccc agggcggcga ctgggggatc tgtgaaatca gcttgttcag
    37021 cggtgatcaa ctgatgagcc agctccgcgc acagaaccat ttatataccg tgctggagaa
    37081 aggtgcggac ggcaatcagg tgataattgt cggtgccgtt cacgaatgcc ttaatgcaaa
    37141 actggattcc ttagcggcaa ttattgagaa attttgcgag ccacaggtgg caattgtttc
    37201 cctgacgatt accgaaaaag gctattgtat tgacccggcc accggtgcac tcgacaccag
    37261 taatccgcgg attattcacg atctacaaac ccctgaagaa cctcactccg caccgggtat
    37321 tctcgtcgaa gcactgaaac gccgccgtga gcgcggcctt acaccgttta ccgtgctctc
    37381 ctgcgacaat attcccgaca atggccatgt ggtgaaaaac gcggtgctgg gaatggcaga
    37441 aaaatgttcg ccagaactcg ccgggtggat aaaaaaacac gtcagttttc cgggaaccat
    37501 ggtcgaccgc attgttccgg ctgcaaccga cgaatcactg gcagaaatca gccagcatct
    37561 gggggtaaat gatccctgcg cgattagctg cgaaccgttt atccagtggg tggttgaaga
    37621 taacttcatc gctgggcgtc ctgcctggga agtcgcaggt gtacaaatgg tgaatgatgt
    37681 cctgccatgg gaagagatga aactgcgtat gcttaatggc agccactctt ttctcgctta
    37741 tctgggttac ctctcaggat tcgtccatat cagtgattgc atgcaggatc gcgtatttcg
    37801 ccatgctgcc agaacattaa tgctggatga gcaagcgccg acactgcaaa ttaaagatgt
    37861 cgatttaaca caatatgcgg ataagttaat tgcacgtttt gctaatccgt cgctgaaaca
    37921 taagacctgg caaatcgcga tggatggcag ccagaaatta ccgcaacgta tgctggcagg
    37981 tattcgcata catctggggc gtgaaacgga ctggtcgttg ctggcattag gcgttgctgg
    38041 ctggatgcgt tacgtcagcg gcgttgatga tgccggaaat gccattgatg ttcgcgatcc
    38101 gcttagcgat aaaattcgcg aacttgttgc gggcagcagc agtgaacaac gcgtaaccgc
    38161 cctgctttcc ctgcgtgaag ttttcggtga tgatctgcca gataacccgc attttgtgca
    38221 ggccatcgaa caagcctggc aacaaatcgt acaattcggc gcacatcagg cgctattaaa
    38281 caccctcaaa atttaacgat ttctgcggtt aaagcggatg aagctcacct tcgtccgctc
    38341 tccccttctc ttttctgcct tttttagcca ggattaacgc tcagttaact taccagagta
    38401 ttgcggagcc gttgtgacca ggaccaacct catcaccggt tttctcggca gcgggaaaac
    38461 cacgtcgatt cttcatctgt tagcccataa agatcccaac gaaaaatggg cggtactggt
    38521 taatgaattt ggggaagtcg gaattgatgg tgctttgctc gccgatagcg gcgcattgct
    38581 gaaagagatc cccggcggct gcatgtgctg cgttaatggt ttacccatgc aggtagggtt
    38641 gaatacctta ctgcgtcagg gaaaaccaga ccgcttgttg atagagccga ccgggctggg
    38701 ccatccgaaa cagatcctcg atctgttaac cgcaccagtc tatgaactgt ggatagatct
    38761 gcgcgccacc ttgtgcattc tcgatccacg cctgctgctg gacgaaaaaa gcgccagcaa
    38821 tgaaaacttc cgtgaccagc tggctgccgc agacatcatt gtcgccaata aatccgaccg
    38881 tgcgacgccc gaaagtgagc aagcgctaca gcgttggtgg cagcaaaatg gtggcgatcg
    38941 acaattaatt cacagtgagc atgggaaagt tgacggtcat cttctggatt tgccgcgtcg
    39001 caatttagcc gagttgcccg ccagcgccgc gcattctcat cagcatgtcg tgaaaaaagg
    39061 gttagcagcg ttaagcctgc cagagcatca acgctggcgt cgcagtctga acagcgggca
    39121 aggatatcag gcctgcggct ggatattcga cgctgatacg gtattcgaca ccattggcat
    39181 tctggaatgg gcgcgacttg caccggtgga acgcgtcaaa ggcgtgctgc gtattcccga
    39241 agggctggta cgaatcaacc gtcagggcga tgacctgcac attgaaacgc aaaacgttgc
    39301 gccaccggac agccgtattg agctgatttc cagcagcgaa gctgactgga atgctttgca
    39361 gagcgcgctg ttgaagcttc gtttagcgac taccgcgtaa ggttgcctgc gtttttcagt
    39421 aagataatta gagaaaatat gattaaaaat ttgccgcaaa tagtgttgtt gaatattgtc
    39481 ggcctcgcgc tgtttctttc ctggtatatt cccgttaatc atggattctg gttgccgatt
    39541 gatgcggata ttttttattt ctttaatcag aaactggtcg aaagtaaggc ctttttgtgg
    39601 ctggttgcat tgaccaacaa tcgcgctttc gacggttgtt ctctgctggc gatgggaatg
    39661 ttgatgctga gtttctggct gaaagaaaac gcccctggca gacgacgtat cgtgattatg
    39721 ggtctggtca tgctattaac tgcagtggta ttaaaccagc tgggtcaggc attaattcct
    39781 gtaaaacggg ccagcccaac attgactttt accgatatta accgcgtcag cgaactgctc
    39841 tctgttccca cgaaagatgc ctcacgagat agctttcccg gcgatcacgg catgatgctg
    39901 cttatttttt cggcattcat gtggcgttat ttcggcaaag ttgcaggcct tatcgccctt
    39961 attatttttg tggtttttgc atttcccaga gtaatgattg gcgcacactg gtttactgac
    40021 atcattgtcg gttcgatgac cgtgatattg atcggtttgc cctgggtgtt gctgacgcca
    40081 ttaagtgatc gattaatcac cttttttgac aaatcactac cgggaaaaaa caaacatttc
    40141 caaaacaaat aactcacagt aattaacatc atcaggctta tttttatagt gaggataatc
    40201 ctgatgatgc gcaccgtgct ttcatccatc gcgcgcaaaa atcattctct aagtaaatga
    40261 atggattgca tgcgtttcac tcaatcgtac tttaattgac caatcccgct tattaacttt
    40321 ctgtatcact ttttcttata aaaaatcatg taaaaccgct cgccaagacc gcaccaatcg
    40381 ggtaatctag aactcgtttt gcctcggcgg tagattatcc tcacagcata taattttgtg
    40441 cgttagtcca cagatttggc cttaaggaat tgtttcaaca tgcccaggta attagtctcg
    40501 tgtcgcttgg cattttttta taacgatatt tgtcgttaag gacttcaagg gaaaacaaac
    40561 aacatggtca aatctcaacc gattttgaga tatatcttgc gcgggatccc cgcgattgca
    40621 gtagcggttc tgctttctgc atgtagtgca aataacaccg caaagaatat gcatcctgag
    40681 acacgtgcag tgggtagtga aacatcatca ctgcaagctt ctcaggatga atttgaaaac
    40741 ctggttcgta atgtcgacgt aaaatcgcga attatggatc agtatgctga ctggaaaggc
    40801 gtacgttatc gtctgggcgg cagcactaaa aaaggtatcg attgttctgg ttttgtacaa
    40861 cgtacattcc gtgagcaatt tggcttagaa cttccgcgtt cgacttacga acagcaggaa
    40921 atgggtaaat ctgtttcccg cagtaatttg cgtacgggtg atttagttct gttccgtgcc
    40981 ggttcaacgg gacgccatgt cggtatttat atcggcaaca atcagtttgt ccatgcttcc
    41041 accagcagtg gtgttattat ttccagcatg aatgaaccgt actggaagaa gcgttacaac
    41101 gaagcacgcc gggttctcag ccgcagctaa taaaccgttt ggatgcaatc ccttggctat
    41161 cctgacgagt taactgaaag cactgcttag gcagtgcttt tttgttttca ttcatcagag
    41221 aaaatgatgt ttccgcgtct tgatccaggc tatagtccgg tcattgttat cttttaaatg
    41281 ttgtcgtaat ttcaggaaat taacggaatc atgttcatac gcgctcccaa ttctggacgt
    41341 aagctcctgc ttacctgcat tgtagcaggc gtgatgattg cgatactggt tagctgcctt
    41401 cagtttttag tggcctggca taagcacgaa gtcaaatacg acacactgat tatcgacgta
    41461 caaaagtatc tcgataccta ttttgccgac ctgaaatcca ctactgaccg gcttcagccg
    41521 ctgaccttag atacctgcca gcaggctaac cccgaactga ccgctcgcgc ggcgtttagc
    41581 atgaatgtcc gtacgtttgt gctggtgaaa gataaaaaaa cattctgttc atctgcgact
    41641 ggtgagatgg acattccact caatgaattg attccggcgc tcaacattaa taaaaacgtc
    41701 gatatggcga tcttacccgg cacgccgatg gtgccgaaca aacccgcaat cgtcatctgg
    41761 tatcgcaacc ctttgctgaa aaatagcggt gtgtttgccg ctctgaatct caacctgacg
    41821 ccttctctct tttatagttc acggcaggaa gattacgatg gcgtcgccct cattattggc
    41881 aatactgcgc tatctacctt ttcttcacgt ttgatgaacg ttaatgaatt aaccgacatg
    41941 ccggtccgtg aaactaaaat tgcgggcatt cctctgaccg ttcggcttta tgcggatgac
    42001 tggacatgga acgatgtgtg gtacgcattt ttactgggcg gcatgagtgg aactgtcgtt
    42061 ggcctgctct gctattacct gatgagcgta cgtatgcgcc ccggcagaga aatcatgacc
    42121 gccatcaagc gcgaacaatt ttacgtggcg tatcaaccgg tggtggatac acaagctttg
    42181 cgagtaacgg gcctggaagt actgctacgc tggcggcatc ctgtcgcggg agaaattccc
    42241 ccggatgcct tcattaactt tgccgaatcg caaaagatga ttgtgccgct gactcagcac
    42301 ctgtttgagt taattgcccg cgatgccgca gaattagaaa aagtgctgcc agtaggcgtc
    42361 aaatttggta ttaacattgc gccggaccat ctgcacagcg aaagctttaa agcagatatc
    42421 cagaaactgc tcacttccct gcccgcacac catttccaga ttgtgctgga aattaccgag
    42481 cgcgatatgc tgaaagagca agaagccaca caactcttcg cctggctgca ctcggtcggc
    42541 gtagaaattg ctattgatga cttcggcacc gggcacagcg cgcttatcta tcttgagcgt
    42601 tttacgctcg attatctgaa aattgaccgt ggatttatca acgccatcgg tacggaaacg
    42661 atcacttcac ccgtacttga cgcggtgctg acgctggcga aacgcctcaa tatgctgacg
    42721 gttgctgaag gggtcgaaac gccggaacag gcgcgatggc taagcgaacg cggcgttaat
    42781 ttcatgcaag gctactggat tagtcgccct ttaccgctgg acgattttgt ccgctggctg
    42841 aagaaaccgt atacgccgca gtggtaaggt gtgcttacgt cccttattat tcatagtgaa
    42901 agcatgccgg attgcggcta atgatgagta aaaggaaatc cattgcagat gattgtgcgc
    42961 atactgctgc tgtttatcgc tctgttcacc tttggtgtgc aggcgcaggc tatcaaggaa
    43021 agctatgcct ttgccgtgct gggcgaaccc cggtacgcgt ttaatttcaa ccattttgat
    43081 tatgtgaacc ccgccgcgcc aaaaggtggg cagataacgt tgtcagccct cggcaccttc
    43141 gataatttca accgctatgc actgcgcggc aacccgggcg cacgcaccga gcagctgtac
    43201 gacacgctat ttacgacttc cgatgacgaa ccaggcagtt attacccgct gattgctgaa
    43261 agcgcacgct atgctgacga ttattcctgg gtggaggtcg ctattaatcc gcgcgcccgt
    43321 tttcatgatg gttcgcccat tactgcccgc gatgtagagt ttacttttca aaaatttatg
    43381 accgaaggcg tgccgcaatt tcgtctggtc tacaaaggca ccaccgtcaa agccattgca
    43441 ccgttaaccg tgcgcattga gttagctaaa cccagcaaag aagatatgct gagtctgttt
    43501 tcgctgccga tatttccaga aaagtactgg aaggatcaca aacttagcga cccgctcgcc
    43561 acgcctccgc ttgccagtgg tccgtaccgc gttacgtcct ggaaaatggg gcaaaatatt
    43621 gtctattccc gcgtgaaaga ttactgggca gcaaacttac cggtaaaccg tggacgctgg
    43681 aatttcgaca ccattcgcta cgattattac ctcgatgata atgtcgcctt tgaagcgttt
    43741 aaagcaggtg cctttgattt gcgtatggaa aacgacgcta aaaactgggc cacgcgttat
    43801 accggtaaaa atttcgataa aaaatacatc atcaaagatg agcaaaagaa cgaatcagcc
    43861 caggatacgc gctggctggc gtttaatatc caacgtccgg tattcagcga tcgccgggtc
    43921 cgggaagcaa tcactctcgc ctttgacttt gaatggatga acaaggcgtt gttttacaac
    43981 gcctggagtc gcacgaacag ttattttcag aataccgaat acgcggccag aaattacccc
    44041 gacgccgcgg agctggtgct tctggcacca atgaaaaaag atctaccgcc agaagtcttc
    44101 acacaaatct accagccgcc ggtatctaaa ggcgatggct acgatcgtga caacctgtta
    44161 aaagccgaca aacttctcaa cgaaacgggc tgggtgctga agggtcagca acgcgttaat
    44221 gctacaacgg gtcagccgct cagctttgaa ttattgcttc ccgcaagcag caatagtcag
    44281 tgggtattgc cgttccagca cagcctgcaa cggctgggta tcaatatgga cattcgcaag
    44341 gtggataact cgcaaatcac taaccgcatg cgcagtcgcg actatgacat gatgccgcgc
    44401 ctatggcggg cgatgccgtg gcccagttcc gatttacaga tttcctggtc atcggaatat
    44461 atcaattcca cttataatgc ccccggcgtg caaagcccgg ttatcgactc gctgatcaac
    44521 caaattattg ccgcgcaggg aaataaagaa aaattactgc cgttggggcg agcactggat
    44581 cgcgtattaa cgtggaatta ttacatgctg ccaatgtggt acatggcgga agaccgtctc
    44641 gcctggtggg ataaattctc ccaacccgct gtacgccctg tttacagcct gggtatcgat
    44701 acctggtggt atgacgttaa taaagcggcc aaactgccgt cagccagaca acagggagag
    44761 tagatgggcg cttacctgat tcgccgtctg ttgctggtga tcccaacgtt atgggctatt
    44821 atcactatca actttttcat cgtgcaaatt gcgcctggcg gtccggtcga tcaggccatc
    44881 gccgccattg agtttggtaa tgccggagta ttacccggcg caggcggtga aggtgttcgt
    44941 gccagccatg cgcaaacggg tgtcggcaat atcagcgaca gtaattaccg tggcggacgc
    45001 ggattagacc cagaagtgat cgctgagatc actcatcgct acggtttcga taagccgatc
    45061 cacgaacgtt acttcaaaat gctctgggac tacatccgct ttgattttgg tgatagcctg
    45121 tttcgcagcg cctcggtgct gacgctgatt aaagacagtc tgccggtttc catcaccctc
    45181 ggattgtgga gcacgctgat tatctatctg gtgtcgattc cgttaggcat tcgcaaagct
    45241 gtttataatg ggagtcgctt tgacgtctgg agtagcgcat ttatcatcat cggctacgcc
    45301 attccggcct ttttgtttgc catcctgctg attgtcttct tcgcgggcgg cagctatttc
    45361 gacctgttcc ctctacgcgg cctggtttcc gctaactttg attcgctgcc gtggtatcag
    45421 aaaatcaccg attatctgtg gcatatcacg ctaccggtgc tggcgacagt gattggtggc
    45481 tttgcggcgc tgaccatgct gaccaaaaac tcattccttg atgaagtgcg caagcaatac
    45541 gtggtgaccg cgcgtgcgaa aggggtaagt gagaaaaaca ttctctggaa acatgtgttc
    45601 cgcaacgcca tgctgctggt gattgccggt tttccggcga cgtttatcag catgtttttt
    45661 accggctcgc tgctgattga ggtgatgttt tcactcaatg gtcttggctt actgggctac
    45721 gaagcgaccg tctcgcgcga ttatcctgta atgtttggta ccttgtatat tttcaccctg
    45781 attggcctgc tgctgaatat tgtcagtgat atcagctata cgctggttga tccgcgtata
    45841 gattttgagg gacgttaatg tcgcgactca gccccgtcaa tcaggcccgt tgggcgcgtt
    45901 ttcgtcataa ccgtcgcggc tactggtcgt tatggatttt cctcgttttg tttggtttga
    45961 gtttgtgttc tgaacttatc gctaacgata aaccgttgct ggtgcgttat gacggcagtt
    46021 ggtatttccc gttgttgaaa aactacagcg aaagcgattt tggcggcccg ctggcaagtc
    46081 aggctgatta tcaggacccg tggctgaaac aacggctgga aaataacggc tgggtactgt
    46141 gggcaccgat tcgctttggt gctaccagta tcaactttgc taccgataag cccttccctt
    46201 ctccaccctc ccggcaaaac tggctgggaa cggatgccaa cggcggcgat gtgctggcac
    46261 gcattctcta tggcacgcgg atctcggttc tgtttggcct gatgctgact ctctgttcca
    46321 gcgtgatggg cgtgctggcg ggggcgctac aaggctatta cggcggtaaa gtcgatctct
    46381 ggggacaacg ctttattgaa gtatggtcgg ggatgccgac gctgtttttg attattttac
    46441 tttccagcgt cgtacagcct aacttctggt ggctgctggc aattactgtc ttgtttggct
    46501 ggatgagtct ggtcggcgtg gtgcgggcgg agtttttacg tactcgtaat ttcgactaca
    46561 ttcgtgcggc acaggcgctt ggcgtcagcg atcgcagtat catcctgcgt catatgttgc
    46621 ctaatgccat ggtcgcgacc ctcacctttt taccgtttat tttatgtagt tcgataacca
    46681 ccctgacctc gctcgatttc ctcggcttcg gtctaccgct cggttcaccg tcactcggtg
    46741 aactgctgtt acaagggaaa aataaccttc aggccccgtg gcttgggatc accgccttct
    46801 tgtcggtggc gatattattg tctttgctga tctttattgg tgaagccgtc cgcgacgcat
    46861 ttgatcctaa taaggcggtg tagcatgacg caaactctgt tagcgattga aaatttgtcg
    46921 gtgggttttc gccatcagca aaccgtacgt acagtagtca atgatgtttc actacagatt
    46981 gaggctggcg aaacgctggc gctggtgggt gagtcaggtt caggcaaaag cgttaccgcg
    47041 ctgtcaattt tacgcctgct cccttccccg ccggttgaat atctctccgg cgatattcgt
    47101 tttcatggcg aatcgctgct tcatgccagc gatcaaacgt tgcgcggtgt acgcggtaat
    47161 aagatcgcca tgatttttca ggaaccgatg gtgtcgttaa atccattgca taccctggaa
    47221 aaacagcttt atgaagtgct ttcactccac cgcgggatgc gtcgggaagc ggctcgtggc
    47281 gaaattctta actgccttga tcgcgtcggt atccgccagg cggcaaaacg gctgacagat
    47341 tatccgcatc agctctccgg cggcgaacgc cagcgggtga tgattgcgat ggcgctgtta
    47401 acgcgaccgg aattattaat tgccgatgaa ccgaccaccg cactggacgt ctctgtccag
    47461 gcgcagattt tacagctgtt gcgcgaactg caaggcgagc tgaatatggg catgctgttt
    47521 attactcata acctcagcat tgtcagaaaa ctggcccacc gcgtggcggt aatgcaaaac
    47581 ggtcgctgtg tcgagcaaaa taacgccgct acgctatttg cctcacccac tcatccttac
    47641 acacaaaagc tactcaacag tgaaccgtca ggcgatccgg taccgttgcc agaacctgcc
    47701 tcaacgttgc tggatgttga acagcttcag gttgccttcc ccattcgcaa agggattttg
    47761 aagcgcattg tggatcataa tgtggtggtg aaaaacatca gttttacgct acgggcgggt
    47821 gaaacactgg gtttagtggg cgagtctggt tccgggaaaa gcacgacggg actggcgctg
    47881 ctgcgactga ttacctctca gggcagcatc gtctttgacg gtcagccact gcaagattta
    47941 aatcgccgcc agctgttacc tactcgtcat cgtattcagg tggtatttca ggatccaaac
    48001 tcctcgctca acccacgact caacgttttg cagattattg aggaaggctt acgggttcac
    48061 cagccgacgc tttctgccgc acaacgcgaa caacaagtga tagccgtgat gcatgaagtg
    48121 ggattagatc ctgaaacacg ccaccgttat ccggcggagt tctctggtgg tcagcgacaa
    48181 cgtattgcga ttgccagggc attaattctt aagccctcac tgatcatact tgatgaaccg
    48241 acatcatcac tcgacaaaac ggtacaggcg caaatattga cgctattgaa atcattgcaa
    48301 caaaagcatc aactggccta tttgtttatc agccacgatt tgcacgttgt ccgcgcgtta
    48361 tgtcatcagg ttatcgtact gcgacaaggg gaagtagtgg aacaaggacc gtgcgcgcgc
    48421 gtgtttgccg caccgcagca ggagtatacg cgtcagctac tggcgttgag ctgacgctta
    48481 aaaaggattg tagtttgaaa agggttcggc aattgccaca ccaaaatttt tcaatcggca
    48541 tgttgcagca aactcgtcct ggcgatttac aaacaggcac ggctcacctt cacactccag
    48601 caccgaacat ggaacttcga tgtcgcttaa cgcctggctt agtttataca tcaccgacca
    48661 tgcattatca ccatccgggc taagcaattt aagcgccacc aggctgttgt caccgcacgg
    48721 tccattttgc ggaatcggtt caaccttcga acctgcgaaa ccagcagacc agcgaaagtt
    48781 ctgcggtaga cgatggacga ttgagagttg taatgaagtc acttattttc cccggaagca
    48841 catttacttc acaatttatt tacatcaatt ttaacacatc atcaacaatc cgtctttata
    48901 cagatcgtaa agattgatgc ctcgcctgca tggcctctat ggctctgatt taagtaatat
    48961 ctgcgggcgt ggtcacgttt gcggggctat atgtacccgt ttctgcgatc taactcaacc
    49021 tttttaacta caatgatgtg actttttaca taaattgatt ttacataaaa taaacatata
    49081 tcgggggaat gatagatttg tggttgacat gcatcaacaa aggaagcctt ttagcttcct
    49141 cgttgtgcaa tagatcaccg ttttttcggt cgactggcgt acagacagaa gagaatggag
    49201 ctggttgcgc agaatgcaat tgaccaaatc atcggccatg cagagttaaa ggtcgcaaga
    49261 gaaagcaatg cgccaacaat tgcccctatg ccaaaacgga aggttcctgc cagcgaagat
    49321 gccgttcccg ccatatgggg aaactcatca agaatgaccg ccatcgcatt ggatgacacc
    49381 atagacacgc agcccacaaa cgccgcaacg ccaaccacca gcgaccagaa tcccagcccc
    49441 agcagcgcac tgatgaccat ccacgctgcc ataataaatt gtatccacaa ccccgagcgg
    49501 aacatattta acgcgccaat gcggcggacg aagcggctgt taaagatggt catcacgaac
    49561 agaaaaacaa tgtttagcgc aaagtaataa ccaaagtttt ccggtgcaac gtggttgatt
    49621 tcaatgtaga caaacggtcc ggcgctcagg aacgagaaca ttccggcaaa gctgaaaccg
    49681 ctggccagca tataactcag gacacgctta tgacggaaca gcgccgcaaa gttaccaata
    49741 gtggtacgaa tgtgaaaagg ctggcgagga tccagaagat gtaatgccag ctcagccaca
    49801 ccagcaccca gccgccaact atcggtgcca tcagcggtgc aatggtcgtc accagcatga
    49861 caaacgacat catccgcgag aactcttctt tcgggtaaat atcacgcatc aaagcgttga
    49921 taaccacgct ggccgcagcc gcagccagcc cgtggaagaa acgcatcaca atcagctgat
    49981 cgatggtttg tgccaacgca cacgccaccg cggcggcggc aaacaccagc gtaccgccaa
    50041 gaaccaccgg cttacgcccg aagctgtctg ccatcggccc gtagattaac tgccccaacg
    50101 caaagcccag aatataagta ctcagggtca tctgcgtact gcccgccggt acgccaaact
    50161 gcgctgaaat taccggtagc gcgggcagat acatatcaat cgacagcggc atcaacatgg
    50221 ccagcaggcc aaggataaaa acaatagcaa acgacgaatg ctgtcgggtg gtcacaacgg
    50281 gctcctgaaa gtcattgaaa agttagacga cgctggcaat ttcttcttca gttaacggac
    50341 gatattcacc gggggctaaa tcagcatcca gcgtaatgcc gccaatacgt tcacgatgca
    50401 gctcaaccac atggttaccc acggcggcga acatgcgttt cacctgatga taacgccctt
    50461 cgctgatggt cagacgaacc tgcgttgggg taatcacttc cagcaccgcc ggcttagtga
    50521 gatctttttc gttatgcagc tgcacgcctt tagcaaattg ttctgccgta tcgtcagcta
    50581 cgggtgatac cagcgtcacc agataggtct tctcgcaatg atggcgcgga gaagtaatgc
    50641 ggtgcgacca ctgaccatca tctgtcatta gtaccagacc ggtggtatcg atatccagcc
    50701 gccctgcggc gtgcagtttc cacgctaccg gttcatctag aaaatagagc accgttgggt
    50761 gatcagggtc gtccgtggag caaacataac cctgaggctt attgagcatg aagtaacgtg
    50821 gaccgtgttg ctgcgccagc gggttgccat cgtaagcgac atcatgttca ggaagcagtt
    50881 tgaacgctgc attacggacg atttcgccat cgacggtgac acgattgccg cggatttcac
    50941 gcccggcaat agcacggcta acgccgagtt gctgtgcgat aaatttatca agtcgcatgt
    51001 gtgtgatttt gcctgtaaaa aacggaggtc gggtaatgac ccgaatatct gaactgttgt
    51061 ctgcccagta tagcggtcta ataacatccc tcaagggaaa aagatccgtg gcatactatt
    51121 agcagaataa tctacctacg cgagaccatg atttttacac ttcgcccata tcagcaagaa
    51181 gccgtggatg ccacgctcaa ccattttcgt cgtcataaaa cccctgccgt tatcgtgctg
    51241 cccaccggtg caggtaaaag cctggtgata gcggaactgg cacggctggc tcgtggtcgc
    51301 gtactggtgc tggcacacgt taaagaactg gtggcgcaaa accatgcaaa gtatcaggcg
    51361 ctggggctgg aagccgatat ttttgccgcc gggctgaagc gtaaagagag ccacggtaaa
    51421 gtggtatttg gcagtgtgca gtcggtcgcc cgtaatattg atgcctttca gggtgaattt
    51481 tcgctgttga ttgtcgatga atgtcaccgt attggtgacg atgaagagag ccagtatcag
    51541 caaatcctca ctcacctgac aaaagtgaat ccccacttac gcctgctggg gctgactgcc
    51601 acgccttttc gattgggcaa aggttggatc taccagttcc attatcacgg catggtacgc
    51661 agcgatgaga aagccctttt ccgtgactgc atttatgagc tgcccctgcg ttatatgatt
    51721 aaacacagct atctgacgcc gccagaacga ctggatatgc cagtagtgca atatgatttc
    51781 agccgcttgc aggcacagag caacgggctg ttcagcgaag ccgatctcaa ccgtgagctg
    51841 aaaaaacagc aacgtattac cccgcacatc atcagccaga ttatggagtt cgccgaaaag
    51901 cgtaaaggag tgatgatttt cgccgcgacg gtcgaacacg caaaagagat tgtgggatta
    51961 ctgcctgccg aagatgcagc actgattact ggcgacaccc ccggcgctga gcgcgatgtg
    52021 ttaattgaaa attttaaagc ccagcgtttt cgctatctgg tcaacgtcgc ggtactgacc
    52081 accggatttg acgccccgca cgtcgatctt atcgccattc tgcgccctac cgaatcagtg
    52141 agtctttacc aacaaattgt cgggcgcggt ctgcgtctcg ctccgggcaa gactgattgt
    52201 ttaattcttg attatgcggg taatcctcac gatctctacg cgccggaagt tggtacaccg
    52261 aaaggcaaaa gtgacaacgt tccggtacag gttttctgcc ctgcctgcgg ttttgccaac
    52321 accttttggg ggaaaacgac cgccgacggg acattgattg aacactttgg tcgccgctgt
    52381 cagggatggt ttgaagatga cgacggtcat cgcgaacagt gtgacttccg tttccgtttt
    52441 aaaaattgcc cgcaatgtaa cgcagaaaat gatattgccg ctcgccgctg ccgcgaatgt
    52501 gacacggtac tggttgatcc ggatgatatg ttaaaagcgg cgctacgact gaaagatgcg
    52561 ctggtattac gctgtagcgg catgtctttg cagcatgggc acgacgagaa aggcgaatgg
    52621 ttgaaaatca cctattacga tgaagacggt gcggatgtga gtgagcgttt ccgtctgcaa
    52681 acacctgccc agcgtaccgc cttcgagcag ctttttatcc gcccgcatac gcgcacaccg
    52741 ggcatcccgc tgcgctggat caccgccgcc gatatcctcg cccagcaagc cttattgcga
    52801 cccccggatt ttgtcgtcgc ccgcatgaaa ggccagtact ggcaggtgcg tgaaaaagtg
    52861 ttcgattacg aaggtcgttt tcgtcgggcg cacgaattac gcggttaatg gtagttttga
    52921 ttgatgtata aggcgattga gtatagaatc tcacccgctt ttgcatacgc aaagcagatc
    52981 acttacctgt tgctgggtcg cctgtagcag gattaattta agagagaaag aaatgtttac
    53041 tatcaacgca gaagtacgta aagagcaggg taagggtgcg agccgccgcc tgcgtgccgc
    53101 taacaagttc ccggcaatca tctacggtgg caaagaagcg ccgctggcta tcgagctgga
    53161 tcacgacaaa gtcatgaaca tgcaagctaa agctgaattc tacagcgaag ttctgaccat
    53221 cgttgttgac ggtaaagaaa tcaaagttaa agctcaggac gtacagcgtc acccgtacaa
    53281 accgaagctg cagcacatcg acttcgttcg cgcttaattg ctgaataagt tgtaaaaaac
    53341 cccgctcccg cggggttttt tgtatctgca gattatgcct gattacggta ttgctatttt
    53401 ttgcaggcca gataaggcgt tcacgccgca tctggcattc agaacacaaa accgattaat
    53461 tgccgccaga cgtccggcgc tgcaattggt cgcgcaaatt cggcggcgtg cctttaatgg
    53521 tcagggtatc ggtcgccggc tcccagaaga tgcgttcacc taacagcatc gcatcaaagt
    53581 taatagtcaa gccaccgccg ctaccggcaa acttcgtcaa ctgacgcagt gtgctccggt
    53641 ctgccgggaa gctctcttcc agttcgtagc ctttttcagc cgcaaactcc gtgaagctca
    53701 cttcgcttac gcctgccagc tctttcgaca gcgattccag ttcgatctct tcgcctgcct
    53761 gcagctgctc attacagtag ctataaacct gctggcgcac gttctgtcgc tctgctttat
    53821 ccagttgtgc ttcggcagtg aaatcatcaa ccgcttgcaa cagcccacgg ttttgtgctt
    53881 tcgtgttcaa tccttcgctg gccccgagga aatccataaa gaaatccgcc actttgcgcc
    53941 ctactcgccc tttcaggaaa gtgagatagc gggtggactc tggattggtt tcccattcgg
    54001 ttaaatcgat acgcgcaaca atatccgcat gattgatatc aagataatgg gttgggttga
    54061 tgtcgagatt ttcgttaaca cgcatactgc tcaggttgct cagaactgcc accagcagat
    54121 actccaccgc cagataacgg tagtggcaaa acagcacgaa tccaccgtca gcaaaaggat
    54181 atttcgctaa ttcgtcacgc aggcgaccag ttgccgcgcg gctaaatgcc agaaagtcct
    54241 cttcgccctg acgctgtaag cgcagcgtct gcgccagttc actctcttcg ctaaacaagc
    54301 cgtaagcttt atttttggcg ctatagaccc gatgcagttc tgccaccatc tcaacgacgg
    54361 tttctgtcgg ttccagcaat gaatcgcgca acaccagctc aaggttctgc tcatcacgct
    54421 tgataagctg gtgcagagca atctggttga tatccagact catgataaac tctcctttaa
    54481 gaccgggcgg tattcaacca ccgccggtta agcgacgcaa taaaagatgg cggtcatttt
    54541 acagaaggca gcgtaacgag aatgatttaa cgcataaaaa agcagaaaaa aaaccgttgc
    54601 tacggtaata tgttgccctt tcatcaacta actgatttcg atttatgcca caaatttccc
    54661 gctacagtga tgaacaggtt gaacaattgc tcgcagagct gctcaacgta ctggaaaaac
    54721 ataaggctcc gaccgacctt tccctgatgg tgttaggcaa catggtcact aaccttatca
    54781 acaccagcat tgccccggcc caacgccagg caattgccaa ctcttttgcc cgcgccttac
    54841 agtcctctat caacgaagac aaagcgcact aagggaaaca gataacaggt tatggtaact
    54901 catcgtcagc gctaccgtga aaaagtctcc cagatggtca gttgggggca ctggtttgca
    54961 ctgttcaata ttctgctttc gctcgtcatt ggcagccgtt acctgtttat cgccgactgg
    55021 ccgacaacgc ttgctggtcg catttattcc tacgtaagca ttatcggcca tttcagcttc
    55081 ctggtgttcg ccacctactt gctgatcctc ttcccgctga cctttatcgt cggctcccag
    55141 aggctgatga ggtttttgtc cgtcattctg gcaacggcgg gaatgacgct attactgatc
    55201 gatagcgaag tctttactcg tttccatctc catcttaatc ccatcgtctg gcagttggtt
    55261 atcaacccag acgaaaatga gatggcgcgc gactggcagc tgatgttcat cagcgtgccg
    55321 gttattttat tgcttgaact agtgtttgcg acgtggagct ggcaaaagct gcgcagcctg
    55381 acgcgtcgtc gacgcttcgc gcgcccgctg gccgcattct tatttatcgc ctttatcgcc
    55441 tcgcatgtgg tgtatatctg ggccgatgcc aacttctatc gcccgatcac catgcagcgc
    55501 gctaacctgc cgctttcgta cccgatgacg gcgcgacgtt ttcttgagaa gcatggtctg
    55561 cttgatgcgc aggagtatca acgccgtctt attgagcaag gtaatccaga cgccgtttcc
    55621 gttcagtatc cgttaagcga actgcgctat cgcgatatgg gcaccgggca gaatgtgctg
    55681 ttgattactg tcgatggcct gaactactca cgcttcgaga agcagatgcc tgcgctggca
    55741 ggttttgctg agcaaaatat ttcgttcacg cgccatatga gctccggcaa cactacagac
    55801 aacggcatct ttggcctgtt ctatggtatc tcgccgagct atatggacgg cattctgtcg
    55861 acccgtacgc ctgcggcatt aattactgcg cttaatcagc aaggctatca gctggggtta
    55921 ttctcatcag atggctttac cagcccgctg tatcgccagg cattgttgtc agatttctcg
    55981 atgccgagcg tacgcaccca atccgacgag cagaccgcca cgcagtggat caactggctg
    56041 ggccactacg cacaagaaga taaccgctgg ttctcgtggg tttctttcaa tggcactaac
    56101 attgacgaca gcaatcagca ggcatttgca cggaaatata gccgggcggc aggcaatgtc
    56161 gatgaccaga tcaaccgcgt gctcaatgca ctgcgtgatt ctggcaaact ggacaatacg
    56221 gttgtgatta tcactgccgg tcggggtatt ccgctgagcg aagacgaaga aacctttgac
    56281 tggtcccacg gtcatctgca ggtgccatta gtgattcact ggccaggcac gccggcgcag
    56341 cgtattaatg cgctgactga tcataccgat ctgatgacga cgctgatgca acgcctgcta
    56401 catgtcagca cacctgccag cgaatattcg caaggtcagg atttgttcaa ccctcaacgc
    56461 cgtcattact gggttactgc agcggataac gatacgctgg caattaccac cccgaaaaag
    56521 acgctggtgc tgaacaataa cggtaaatac cgcacttaca acttacgtgg tgaaagagtg
    56581 aaagatgaaa aaccacagtt aagtttgtta ttgcaagtgc tgacagacga gaagcgtttt
    56641 atcgctaact gattaattat aaatcagtta gcgaaatatc ttacttgcaa tcggtgtgga
    56701 aaacggtagt attagcagcc acgagtcggc acgtagcgca gcctggtagc gcaccgtcat
    56761 ggggtgtcgg gggtcggagg ttcaaatcct ctcgtgccga ccaaaaatcc caagaaaaaa
    56821 ccaaccttta cggttggttt ttttatatct gcaattaatt tgataaacag accgtgacac
    56881 atcacagcct gtttattttc tgttatcaga acgtccagac cactcccgcc tgagtattcc
    56941 acggcgattc tacccctgcg ccattgccat agctgactga caaatgaccg cttaacgtcg
    57001 gggtaaacga tgaccttatc ccagcctgat aaacgccacg ggttccagcc acatcattat
    57061 taaatttgcc atcgtcatta actttcacat ggttagaatc ggcgtattcc tgacgcacgg
    57121 cggctttcag ccagggttcc aacgtcgtac cgttttgcag gtccatgtga tagcttaccg
    57181 ccgttcccgc ttcagcgcgt aatatccggg tatttcccac atccgcgcgc atgccgtttg
    57241 ataacgtgta gtcctgacca tctgtggtaa agccggtaaa ggcgagatag ggtctaacac
    57301 tccacaatcc gtcaacccaa cggaacccgc tctcaacatg agcacccgcg ccgttactat
    57361 tgtaatcgcc aaacgctgtt gccccattac tcatcttgcc atggatggtg ttggcaaaac
    57421 ggtcaacttt caccacccca tcaacatagg caccgttctg atgctcccaa ccggcataag
    57481 cccccagggt gtagctatca atattgcctt tgccgccgcg atcaaaacca atatcagaat
    57541 gagagtaatc aaagaacaag ccgcgaattg tgctgctttc ttcacgggag aaacggctat
    57601 cgataccgag cgtcaggccc gtcaatgttt gctcaaagcc agctcccgca tcagtggtca
    57661 cgttgttgcg ggtgttaatt gccgaactcc acatcgccgt atcgtaatta acgcctttta
    57721 cgctaccaag acgctcacgc acggtgtcca gttctgcatc aaataccagc ggttgtgctg
    57781 ctgccatgtt cagcacatca gtggttgaag gggtaatttg cgcgcgattc tctgcgaggc
    57841 tccagctatg gttgccatta tccagcaagg tatattcata cgtaccgata tcaacaacgc
    57901 ctccggcatt gcccaacgtg aatgcagcat caccgccgcc cgtcgttacc agtgtaaggc
    57961 tatctcctgc tgccgggctg gcccccgtgt ccgtcacgaa tattttgaaa tcacctgttg
    58021 cctgaccgat gacgttgagc tgatcgctct gatgattagc catatcggta cgcatataaa
    58081 attgccgttt cccgacaggg tattggtggt caacgtaatg aaattttccg cacttgccgt
    58141 cacagatgaa cgagtcactg gctcagccat cataatcaca tttgcgccgg acatcgcaag
    58201 gttggctaag ttgaggcgag tgttttggtt cgtcgtagtg atatcggcaa tgatggtcca
    58261 tgcgctattt tcgagcgaaa cgtccgcttg gctggtatcc acggcaccat gcgttctaaa
    58321 agaagcgccc tcagaaacca ctaccttacc caccaacata gcgggtgcat aatttatttt
    58381 cggcttcatg acctcaagaa tgccatcatt ccctctgact gacacgttaa ccattgtgcc
    58441 agcccagaca ttagcgcggc cattgttgat aaccatattt tcagagatgg ctttagagga
    58501 atacgtgata cttccattct gatctgatcg accgagatca tatacagcat ttgcctgcac
    58561 tttcgtacca gtatcctttc ccagcgattg catagtggca tgctcatcaa ggatagtgtc
    58621 aatggcctga gtgtcctcca taacaatgag cccggagcca tcatccagtt cataattttt
    58681 tgacacacca tcctttatac taaattggcc tttactgttt gtaccactca cttccagcgc
    58741 attcgttgag acaattaatt tcccgcctgc ttttttatca acgccagttg cagtcccgcc
    58801 atccataacc tccagtaatc ctcccttttc gatagcggca aagacggcaa tcccatcttt
    58861 ttcaacaatt aagcgcccgc ctttaatcgt gggatcattc gccgc
//
LOCUS       DAJTZL010000004        58385 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.004, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000004 DAJTZL010000000
VERSION     DAJTZL010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 58385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58385
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.004"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..160
                     /locus_tag="OO544_000222"
     CDS             <1..160
                     /locus_tag="OO544_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HCR6765923.1"
                     /translation="WQLAHSSADNFPALTVSHFITANLLPVMLGNIIGGAVLVSMCYR
                     AIYLRQES"
     gene            complement(198..1259)
                     /locus_tag="OO544_000223"
     CDS             complement(198..1259)
                     /locus_tag="OO544_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR6765924.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            1472..2935
                     /gene="bepA"
                     /locus_tag="OO544_000224"
     CDS             1472..2935
                     /gene="bepA"
                     /locus_tag="OO544_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HCR6765925.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSQQQARYDARIDQLRQLQERFKPYTKM"
     gene            2956..3315
                     /gene="arsC"
                     /locus_tag="OO544_000225"
     CDS             2956..3315
                     /gene="arsC"
                     /locus_tag="OO544_000225"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HCR6765926.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKKNGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(3453..4199)
                     /gene="hda"
                     /locus_tag="OO544_000226"
     CDS             complement(3453..4199)
                     /gene="hda"
                     /locus_tag="OO544_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HCR6765927.1"
                     /translation="MVNFSRFCEILVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSS
                     LLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVP
                     EVLDGMEHLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNL
                     GLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRT
                     LFMTLDQLDRASITAQRKLTIPFVKEILKL"
     gene            complement(4249..5538)
                     /gene="uraA"
                     /locus_tag="OO544_000227"
     CDS             complement(4249..5538)
                     /gene="uraA"
                     /locus_tag="OO544_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HCR6765928.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            complement(5624..6250)
                     /gene="upp"
                     /locus_tag="OO544_000228"
     CDS             complement(5624..6250)
                     /gene="upp"
                     /locus_tag="OO544_000228"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HCR6765929.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            6575..7612
                     /gene="purM"
                     /locus_tag="OO544_000229"
     CDS             6575..7612
                     /gene="purM"
                     /locus_tag="OO544_000229"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HCR6765930.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            7612..8250
                     /gene="purN"
                     /locus_tag="OO544_000230"
     CDS             7612..8250
                     /gene="purN"
                     /locus_tag="OO544_000230"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HCR6765931.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTIRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            8421..10487
                     /gene="ppk1"
                     /locus_tag="OO544_000231"
     CDS             8421..10487
                     /gene="ppk1"
                     /locus_tag="OO544_000231"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HCR6765932.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYVASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            10492..12033
                     /gene="ppx"
                     /locus_tag="OO544_000232"
     CDS             10492..12033
                     /gene="ppx"
                     /locus_tag="OO544_000232"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HCR6765933.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(12072..14315)
                     /gene="pdeF"
                     /locus_tag="OO544_000233"
     CDS             complement(12072..14315)
                     /gene="pdeF"
                     /locus_tag="OO544_000233"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HCR6765934.1"
                     /translation="MKLNATYIKMRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGIALGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYMDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNAPENMQRRGAMYLQRELKDKVAMMNRLQQALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELIVPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQLLAKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
     gene            complement(14497..14649)
                     /locus_tag="OO544_000234"
     CDS             complement(14497..14649)
                     /locus_tag="OO544_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6765935.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKTSVDTYFNVMEVNLIIYND
                     DVIMMN"
     gene            14667..14858
                     /locus_tag="OO544_000235"
     CDS             14667..14858
                     /locus_tag="OO544_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2633 family protein"
                     /protein_id="HCR6765936.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
     gene            15169..15687
                     /locus_tag="OO544_000236"
     CDS             15169..15687
                     /locus_tag="OO544_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HCR6765937.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            15703..16242
                     /locus_tag="OO544_000237"
     CDS             15703..16242
                     /locus_tag="OO544_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HCR6765938.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLTLAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(16337..17914)
                     /gene="guaA"
                     /locus_tag="OO544_000238"
     CDS             complement(16337..17914)
                     /gene="guaA"
                     /locus_tag="OO544_000238"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HCR6765939.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWARLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(17983..19449)
                     /gene="guaB"
                     /locus_tag="OO544_000239"
     CDS             complement(17983..19449)
                     /gene="guaB"
                     /locus_tag="OO544_000239"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HCR6765940.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            19611..20981
                     /gene="xseA"
                     /locus_tag="OO544_000240"
     CDS             19611..20981
                     /gene="xseA"
                     /locus_tag="OO544_000240"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HCR6765941.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     TRQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRTGQQQQRLTQRLNQQNPQPKIHRAQTRIQQLEYRLAETLRVQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKAGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            complement(20978..21193)
                     /locus_tag="OO544_000241"
     CDS             complement(20978..21193)
                     /locus_tag="OO544_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HCR6765942.1"
                     /translation="MELHCPQCQHVLDQDNGHARCPSCGEIIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(21262..22734)
                     /gene="der"
                     /locus_tag="OO544_000242"
     CDS             complement(21262..22734)
                     /gene="der"
                     /locus_tag="OO544_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HCR6765943.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLERVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDTNVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            complement(22852..24030)
                     /gene="bamB"
                     /locus_tag="OO544_000243"
     CDS             complement(22852..24030)
                     /gene="bamB"
                     /locus_tag="OO544_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HCR6765944.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(24041..24661)
                     /gene="yfgM"
                     /locus_tag="OO544_000244"
     CDS             complement(24041..24661)
                     /gene="yfgM"
                     /locus_tag="OO544_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HCR6765945.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGKALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(24679..25953)
                     /gene="hisS"
                     /locus_tag="OO544_000245"
     CDS             complement(24679..25953)
                     /gene="hisS"
                     /locus_tag="OO544_000245"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HCR6765946.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGIFEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(26064..27182)
                     /gene="ispG"
                     /locus_tag="OO544_000246"
                     /gene_synonym="gcpE"
     CDS             complement(26064..27182)
                     /gene="ispG"
                     /locus_tag="OO544_000246"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HCR6765947.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            complement(27209..28222)
                     /gene="rodZ"
                     /locus_tag="OO544_000247"
     CDS             complement(27209..28222)
                     /gene="rodZ"
                     /locus_tag="OO544_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HCR6765948.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPVPTAATTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            complement(28507..29661)
                     /gene="trmG/rlmN"
                     /locus_tag="OO544_000248"
     CDS             complement(28507..29661)
                     /gene="trmG/rlmN"
                     /locus_tag="OO544_000248"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HCR6765949.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            complement(29811..30242)
                     /gene="ndk"
                     /locus_tag="OO544_000249"
     CDS             complement(29811..30242)
                     /gene="ndk"
                     /locus_tag="OO544_000249"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HCR6765950.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            complement(30391..32703)
                     /gene="pbpC"
                     /locus_tag="OO544_000250"
     CDS             complement(30391..32703)
                     /gene="pbpC"
                     /locus_tag="OO544_000250"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HCR6765951.1"
                     /translation="MPRLLTKRGCWITLAAAPFLLFLAAWGADKLWPLPLHEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEAA
                     RNKVLERMAAQGVWSREQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEAQPLPDSALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSANLPEDPRPNSVTRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLSEQEG
                     INGIRFPIWLDDKGQRVAADCPQAKQEMVNVWPLPLEPWLPASERRAVRLPPASTICP
                     PYGHDAQLPLQLTGVRDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIATVKFVMQ"
     gene            complement(32704..37665)
                     /gene="yfhM"
                     /locus_tag="OO544_000251"
     CDS             complement(32704..37665)
                     /gene="yfhM"
                     /locus_tag="OO544_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HCR6765952.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     ASSAKLSVPERQKLAQQSAGKVLTLLDLSEVQLDGAATLVLTFSIPLDPDQDFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDYEKTIT
                     TRDIQPSVGFASRGSLLPGKVVEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNPARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLENGAAQQGIEVSLLNEKGQTLTQA
                     TSDAQGHVQLENDKNAALLLARKDGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPGETVILNGLLRDADGKALPDQPIKLDVIKPDGQVLRNVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPNDEVKFSV
                     VGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGLRPQFALK
                     SVYDYRTDSTVKQPIVDEGSNAAFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     DEGWQSQFDQKDLIENEQTLDLQADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGSGAVRPDRVTLKLDKASYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVPAQGLDLTIPVDKTWNRHDLYLSTLVVRPGDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPAKMRPNQPLTVKIKASTKNGEKPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPPVNHVNIVAQQA
                     LSVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADDFGSNESKVIVAAPVIAELNMPRF
                     MASGDTSRLTLDITNLTDKPQKLNVALTASGLLELVSDSPAAVELAPGVRTTLFIPVR
                     ALPGYGDGEIQATISGLALPGETVADQHKQWKIGVRPAFPAQTVNYGTALQPGETWAI
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTASGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFALWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDAINRGNERLLRYLQDPGMMSIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGIALKTMGDATRGEEAIALALKTPRNSDERIW
                     LGDYGSPLRDNALMLSLLEENKLLPDEQYTLLNTLSQQAFGERWLSTQESNALFLAAR
                     TIQDLPGKWQAQTSFSAEPLTGEKAQNSNLNSDQLVTLQVSNSGDQPLWLRMDASGYP
                     QSAPLPANNVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     VGLELENQNLANGSASLEQSGGEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAAEDLLIVRP"
     gene            37872..38717
                     /gene="sseA"
                     /locus_tag="OO544_000252"
     CDS             37872..38717
                     /gene="sseA"
                     /locus_tag="OO544_000252"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HCR6765953.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPVK"
     gene            complement(39210..39986)
                     /gene="sseB"
                     /locus_tag="OO544_000253"
     CDS             complement(39210..39986)
                     /gene="sseB"
                     /locus_tag="OO544_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HCR6765954.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIVPFYERRWGGFLRDFKQNRII"
     gene            complement(40128..41411)
                     /gene="pepB"
                     /locus_tag="OO544_000254"
     CDS             complement(40128..41411)
                     /gene="pepB"
                     /locus_tag="OO544_000254"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HCR6765955.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(41589..41789)
                     /gene="iscX"
                     /locus_tag="OO544_000255"
     CDS             complement(41589..41789)
                     /gene="iscX"
                     /locus_tag="OO544_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HCR6765956.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(41801..42136)
                     /gene="fdx"
                     /locus_tag="OO544_000256"
     CDS             complement(41801..42136)
                     /gene="fdx"
                     /locus_tag="OO544_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HCR6765957.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(42138..43988)
                     /gene="hscA"
                     /locus_tag="OO544_000257"
     CDS             complement(42138..43988)
                     /gene="hscA"
                     /locus_tag="OO544_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HCR6765958.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQAGIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDDAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(44005..44520)
                     /gene="hscB"
                     /locus_tag="OO544_000258"
     CDS             complement(44005..44520)
                     /gene="hscB"
                     /locus_tag="OO544_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HCR6765959.1"
                     /translation="MDYFTFFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(44616..44939)
                     /gene="iscA"
                     /locus_tag="OO544_000259"
     CDS             complement(44616..44939)
                     /gene="iscA"
                     /locus_tag="OO544_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HCR6765960.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(44956..45342)
                     /gene="iscU"
                     /locus_tag="OO544_000260"
     CDS             complement(44956..45342)
                     /gene="iscU"
                     /locus_tag="OO544_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HCR6765961.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(45370..46584)
                     /gene="iscS"
                     /locus_tag="OO544_000261"
     CDS             complement(45370..46584)
                     /gene="iscS"
                     /locus_tag="OO544_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCR6765962.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(46825..47313)
                     /gene="iscR"
                     /locus_tag="OO544_000262"
     CDS             complement(46825..47313)
                     /gene="iscR"
                     /locus_tag="OO544_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HCR6765963.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRIQDAIDVKL
                     RA"
     gene            complement(47614..48351)
                     /gene="trmJ"
                     /locus_tag="OO544_000263"
     CDS             complement(47614..48351)
                     /gene="trmJ"
                     /locus_tag="OO544_000263"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HCR6765964.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKA"
     gene            48470..49273
                     /gene="suhB"
                     /locus_tag="OO544_000264"
     CDS             48470..49273
                     /gene="suhB"
                     /locus_tag="OO544_000264"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HCR6765965.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYSQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            49418..50272
                     /locus_tag="OO544_000265"
     CDS             49418..50272
                     /locus_tag="OO544_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR6765966.1"
                     /translation="MALPVNKRVLKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILAVIGQDDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKETKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            50463..51743
                     /gene="csiE"
                     /locus_tag="OO544_000266"
     CDS             50463..51743
                     /gene="csiE"
                     /locus_tag="OO544_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HCR6765967.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFETRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(51735..52874)
                     /locus_tag="OO544_000267"
     CDS             complement(51735..52874)
                     /locus_tag="OO544_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HCR6765968.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     ALLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAVRQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(53034..53924)
                     /gene="hcaR"
                     /locus_tag="OO544_000268"
                     /pseudo
     CDS             complement(53034..53924)
                     /gene="hcaR"
                     /locus_tag="OO544_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
     gene            54060..55421
                     /locus_tag="OO544_000269"
     CDS             54060..55421
                     /locus_tag="OO544_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HCR6765969.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            55418..55936
                     /locus_tag="OO544_000270"
     CDS             55418..55936
                     /locus_tag="OO544_000270"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HCR6765970.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLEQLDKEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QISETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            55936..56256
                     /locus_tag="OO544_000271"
     CDS             55936..56256
                     /locus_tag="OO544_000271"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HCR6765971.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            56253..57065
                     /gene="hcaB"
                     /locus_tag="OO544_000272"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             56253..57065
                     /gene="hcaB"
                     /locus_tag="OO544_000272"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HCR6765972.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAARYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            57075..58277
                     /locus_tag="OO544_000273"
     CDS             57075..58277
                     /locus_tag="OO544_000273"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HCR6765973.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIAIGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLQADVVIYGIGISANEQLAREANLDTANGIVIDEACRTCDPAIFAGGDV
                     AITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQFIGDM
                     RGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSGKTFDAKLLID
                     ENIALKSL"
BASE COUNT        14271 a        15784 c        14566 g        13764 t
ORIGIN      
        1 ctggcaactg gcgcacagta gcgcagacaa ttttccggca ctgacggtaa gccattttat
       61 taccgccaat ctgctcccgg tgatgctggg taatattatc ggcggtgcag tgctggtgag
      121 tatgtgttat cgggctattt atttacgtca ggaatcctga aaaatcagcc cggcgaaaca
      181 gttcgtcggg ctgaagatta ttcttgcgct atttgcccat cgggccaggc gtggatcacg
      241 gctttgatca gcgtcgccaa tggaatagcg aagaatacgc cccagaatcc ccacaaacca
      301 ccgaagatca ccaccgataa aataatcacc agcggatgca ggttaaccgc ttcggagaac
      361 aacaccggta ctaacagatt accgtccagc gcctgaataa tcagatacac tgcgaagcag
      421 ctccagaatt ccgtgcctgc accaaactgg aatagcgcca cgccaaccac cggaatggtc
      481 accacaaatg cgccaatgta cggaataagt accgagaagc cgaccagcac cgccagcagc
      541 agcgaatagt tcagcccaaa gagcaagaac cccagccaag tggcgatccc caccacgatc
      601 atctccagca ctttgccgcg gatatagttg gtgatttgtt gattcatctc cttccacacc
      661 tgtcctgcca gtccacggtt gcgcggcagc acccgacgaa cggcgttcag catctgctct
      721 ttgtctttca gcaggaagaa gaccatcaat ggtaccagca ccagatagac ggcaatggtc
      781 agcaaaccga ccagcgaggc gagggaaatt ttcaccaccg aatcgcccat ggtcagcatc
      841 cgactgcgca tattttcggc cattgcatca ataatgcccg cgtccattaa tgccggatag
      901 cggcgcggca acgtggcggc aaagtcagaa agcttattga gcatccccgg catatcgcgg
      961 atcaagtaga tgccctgttg ccaggcgata ggcagtacca cgaacgccat cagtagcaat
     1021 ataccgacga aaaccaccaa tacaatcgac gtcgcccagc ggcgggagca gccaatagat
     1081 tgcaggcgca cggttggcca ttccagcaaa taggccagca caatagccac cagcaacgga
     1141 gcaagcaggc cactaaagaa aaagataatg ccaaatccgg caactaaaat aaccagcaag
     1201 gcaatcgctt ccgggtcgct aaaacggcgg cgataccatt gcatcaacat ttcgagcata
     1261 aagaccttcc ctgaacctca agagcgggat tgcgatccgc aattgtatcg aaatgtcaca
     1321 aaaaagactt cgctttttat gacggattca ggaaactgaa aagtcatttg agtgggctaa
     1381 tcttcgccgt tacactcaaa ggcggcgcgg tgggaacaat atttcacagt atcggtcaaa
     1441 tgactatctt cagaaataca ggatagaggt tatgttcagg cagttgaaaa aaaacctggt
     1501 tgcaaccctc attgctgcta tgaccattgg tcaggtagcc ccggcgtttg ccgacagcgc
     1561 agacaccttg ccggatatgg gaacctccgc aggaagcacg ctttccattg gtcaggaaat
     1621 gcagatgggc gactattatg tccgccagct acgcggcagc gcgccgttaa ttaatgaccc
     1681 gctgttaacg caatatatta attcgctggg gatgcgtctg gtttcgcatg ccaattcggt
     1741 taagacaccg tttcattttt ttctgatcaa caacgacgaa attaacgcct ttgctttctt
     1801 tggcggcaac gtggtgctgc actctgccct gttccgttat tccgataacg aaagtcaact
     1861 ggcttcagtt atggcgcacg aaatctccca cgtcacccaa cgtcacctgg cgcgagcgat
     1921 ggaagatcag cagcgcagcg cgccgctgac ctgggtcggc gcgttaggtt ctattttact
     1981 ggcgatggcc agtccgcagg cggggatggc ggcgctgacc ggtacactgg cgggaacgcg
     2041 tcaggggatg atcagtttca cccagcaaaa tgaacaggaa gcggaccgca ttggtattca
     2101 ggtgctgcaa cgctcgggat tcgatccgca ggcgatgcca accttcctcg aaaaattact
     2161 cgatcaggcg cgttactcct cgcgcccgcc ggaaatttta ttgactcacc cgttgccgga
     2221 aagtcgtctg gcagatgccc gcaaccgtgc taatcagatg cgcccgatgg tggtgcagtc
     2281 gtcggaagat ttctatctgg cgaaagcgcg cacactgggg atgtataatt ccggacgtaa
     2341 ccagctcacc agtgatttgc tggatgaatg ggcgaaagga aacgttcgtc agcaacgagc
     2401 ggcgcaatat ggtcgtgctt tacaggcgat ggaagccaat aaatacgacg aggcgcgtaa
     2461 aacgctgcaa ccgttactgg cggcagaacc tggcaacgca tggtatctcg atctggctac
     2521 cgatatcgat cttgggcaaa acaaagccaa tgaggcaatc aatcgcctga aaaatgcccg
     2581 tgatttgcgc accaatccgg tgttgcagct caacctggca aacgcttatc tgcaaggcgg
     2641 tcaaccacaa gaagcggcca atattctgaa tcgctacacc tttaataata aagatgacag
     2701 caacggctgg gatttactgg cacaggcgga agccgcgcta aataaccgcg atcaggagct
     2761 ggctgcgcga gcagaaggtt atgcgctcgc cggacgactc gatcaggcca tttcgctgtt
     2821 gagtagcgcc agttcgcagg tgaaattagg cagccagcaa caagcgcgtt acgatgcgcg
     2881 catcgaccag ttgcgccagc tgcaggaacg ctttaagcct tataccaaga tgtaattcgc
     2941 attaaaggaa gaacaatgac caaacaggta aaaatctacc ataacccacg ctgttcaaag
     3001 agccgggaaa cgctgaacct gctgaaaaaa aacggcgtgg agccagaagt ggtactctac
     3061 cttgagacac ccgccgatgc ggcaacgctg cgcgatttgc tgaaaatgct ggggatgaat
     3121 agcgcccgtg aattgatgcg ccagaaagag gatctttata aagaactgaa cctggctgac
     3181 agttcgctta gcgaagaggc gctgattcag gcaatggtgg ataatccgaa gctgatggag
     3241 cgcccgattg tggtcgcgaa tggcaaagcg cggattggtc gtccaccgga gcaggtactg
     3301 gaaatcgtgg gttaagatgt gccccatcgc tagttgaagc acattgccgg atgcgacgct
     3361 tgccatgtct tatccggcct acgaatacta tggattaact tcgcgtagtt cggataaggc
     3421 gttcgcgccg catccgacaa taaacacctt atctacaact tcagaatttc tttcacaaac
     3481 ggaatggtca gcttacgttg cgcggtaatc gacgcacgat ccaactgatc caacgtcata
     3541 aatagcgtgc gcatttctct gtcgagccgc ttcagcaaga aacgccccac atcttccggc
     3601 agttcaaaac cacgcaaacg cgcgcgtaac tgtagcgcct gcaacttatc ttcatcagaa
     3661 agtggctgca atttgtagat ctgcccccag tcgagtcgcg acgcgagatc cggtaatccc
     3721 agattcaact gccgcggtgg acgatcgccg gtgatcaaca atcgtgtttt gcccgattcc
     3781 agaattcgat tgtagagatc gaaaatcgcc atctcccaca actcatcgcc tgcaatacac
     3841 tcaatgttgt cgatacagac cagcgacaaa tgctccatac cgtcgagcac ttccggaaca
     3901 aaccaggtgc gtttatccag cgggacatag cccaccgcat cgccacgctg cgacaattcc
     3961 gcgcaagccg cgtgcagcag atggctgcgc cccgcgcctt cgcgtgccca gagatagatg
     4021 taaccgctat gttcctgacg cagcacgttt tgcagcgcag ccagtaaaga ggagttatcc
     4081 cccggccaga aacttgcaaa ggtttcgtcg tcaggaagat aaagtggcaa agagagctgt
     4141 gccggtgtgt tcagagatac ctcaaccagg atttcacaaa atcgcgagaa gtttaccaca
     4201 gaatcccatg atgtttgaac cgggcagcaa cactgcccgg tcggtacatt atttgtctgt
     4261 tatatccgcg tcttctgcgt ccagcaccac ttcttcaggg cgcagcacac taatcagttt
     4321 gaagatcaaa cttaagccga taccgacgat ggtcgccagc gccatacctt tcagctccgc
     4381 cgcgccaatg tttaccttcg cgccactgac accgatgatc aaaatcacgg aagtcaggat
     4441 caggttctgt gctttgttgt aatccacttt tgattcgatc aaaacacgaa taccggaagc
     4501 accgatgaca ccataaagca gcagcgaaac gccgcccatc accggcaatg ggatcatctg
     4561 gatagcggca gccagtttac cgacgcagga aagcaggata gcgaaaatcg ccgccccacc
     4621 gataacccag gtactgtaaa cacgggtgat cgccatcacg ccaatgtttt ctccgtaagt
     4681 ggtatttggc gtagagccaa agaagccgga gatcacagtc gacaggccgt tagcaaacat
     4741 cgaacggtgc agacctggat cgcgcagcag atcttttttg acgatattag ccgttactac
     4801 caggtgccct acgtgttcgg caataaccac taacgccgct ggcagaatag tcagaatggc
     4861 aaaccactcg aagcgcggcg tatagagggt tggcagcgca aaccagtgag cattaataat
     4921 cggcgtggta tcgacaattc ccattgcgaa agagagcgcg taccccacca gcacgccaat
     4981 taaaatcggg ataattgcca ggaaaccacg gaacagcacg gaacctaaaa ccgtgaccgc
     5041 cagggtggta atagagatga tgatggtttt ggagtctggc gtttgccctt cagccgggag
     5101 taaacccgcc ataccggcag ctacgcccgc cagctccaga ccgatgacgg caacgattgc
     5161 gcccattgcc gcaggtggaa acagcacgtc cagccagccg gtccccgctt tcttcacgat
     5221 aaaagaaacc aggcagaaca gcacgccgca cataataaag ccgcccagcg cgacttcata
     5281 ccctaacggc aacagtaaca ataccggtga aataaaggca aagctggaac caagataagc
     5341 cggaattttc cctttacaga tgaagagata cagcagcgtt ccaataccgt taaataacag
     5401 tacagtcgcc gggttaatat gaaataagac gggcaccagg acggttgcac caaacatggc
     5461 gaacaaatgt tgcaaactaa gcgggattgt ctgtaaaagt ggcggtcttt cactcacccc
     5521 gatagcacgg cgcgtcatag tattatcctc tgtattatgt gttataggcg ctttactcaa
     5581 aaaaaagccg actcttaaag tcggctttaa ttatttttat tctttatttc gtaccaaaga
     5641 ttttgtcacc ggcatcgccg aggcccggaa taatgtatcc gtgctcgttc agtccctggt
     5701 caatcgatgc ggtatacagt tcgacgtccg ggtgcgcttt ttccagcgca gcgatacctt
     5761 ctggcgcagc taccagcacc agaactttga tgctgctgca gcccgctttt ttcagcaggt
     5821 cgatggtcgc gataacggaa ccaccggttg ccagcattgg gtcaacgatc agcgccatac
     5881 gctcatcgat gttagaaacc agtttctgga agtacggtac cggctccagc gtttcttcat
     5941 tacggtacat accgacaacg ctgatgcgcg cgctcggaac attttccagc acaccgtcca
     6001 tcatccccag accggcacgc agaattggca caacggtaat tttcttacct ttgatctggt
     6061 cgatttctac cgggccgttc cagccttcga tagttacttt ttccgtttcg aggtcggcgg
     6121 tcgcttcgta agtcagcagg ctacccactt cggaagcgag ttcgcgaaag cgcttggtgc
     6181 tgatatcttg ctcacgcatc agtcccagct tgtgtttgac gagtgggtgt ttgacttcca
     6241 cgatcttcat actctttctc ctttgagggg cagccacaaa aaaaatcgac ggattatacc
     6301 tcctttcttc aaggcggcaa tattcttttc gttgacttta gtcaaaatga taacggtttg
     6361 agataaagtt attttatatt caaatggtta tgaaagaaga ttattccatc cgaaaactaa
     6421 cctttgccct ggcacaagtc ttctttcgcc gcgcgtctgg ggaaaagacg tgcaaaaagg
     6481 ttgtgcaaag cagtctcgaa aacgtttgct ttccctgtta gaattgcgcc gaattttatt
     6541 tttctaccgc aagtaacgcg tggggaccca agcagtgacc gataaaacct ctcttagcta
     6601 caaagatgcc ggtgttgata ttgacgcggg taatgctctg gttggaagaa tcaaaggcgt
     6661 agtgaagaaa acgcgtcgtc cggaagtgat gggcggtctg ggcggcttcg gtgcgctgtg
     6721 tgcattgccg caaaaatatc gtgaacccgt gctggtttcc ggcactgacg gcgtaggtac
     6781 caagctgcgt ctggcaatgg acttaaaacg tcacgacacc attggtattg atctggtcgc
     6841 catgtgcgtt aatgacctgg tggtgcaagg tgcagaaccg ctgtttttcc tcgactatta
     6901 cgcaaccgga aaactggatg ttgataccgc ttcagcggtg atcagcggta tcgcggaagg
     6961 atgtctgcaa tcgggctgtt cactggtggg tggcgaaacg gcagaaatgc cggggatgta
     7021 tcacggtgag gattacgatg tcgcgggttt ctgcgttggc gtggtagaaa aatcagaaat
     7081 catcgacggc tctaaagtca gcgacggcga tgtgctgatt gcactcggtt ccagcggtcc
     7141 gcactcgaac ggctattcgc tggtgcgcaa aattcttgaa gtcagcggtt gtgatccgca
     7201 aaccaccgaa cttgatggta agccattagc cgatcatctg ctggcaccga cccgcattta
     7261 cgtgaagtca gtgctggagt tgattgaaaa ggtcgatgtg catgccattg cgcacctgac
     7321 cggcggcggc ttctgggaaa acattccgcg cgtattgcca gataatactc aggcagtgat
     7381 tgatgaatct tcctggcagt ggccggaagt gttcaactgg ctgcaaacgg ctggtaacgt
     7441 tgagcgccat gaaatgtatc gcaccttcaa ctgcggcgtc gggatgatta tcgccctgcc
     7501 tgctccggaa gtggacaaag ccctcgccct gctaaatgcc aacggtgaaa acgcgtggaa
     7561 aatcggtatc atcaaagcct ctgattccga acaacgcgtg gttatcgaat aatgaatatt
     7621 gtggtgctta tttccggcaa cggaagtaat ttacaggcaa ttattgacgc ctgtaaaacc
     7681 aacaaaatta aaggcaccat acgggcagtt ttcagcaata aggccgacgc gttcggcctt
     7741 gaacgcgccc gccaggcggg tattgcaacg catacgctca tcgccagcgc gtttgacagt
     7801 cgtgaagcct atgaccggga gttgattcat gaaatcgaca tgtacgcacc cgatgtggtc
     7861 gtgctggctg gttttatgcg cattctcagc ccggcgtttg tctcccacta tgccgggcgt
     7921 ttgctgaaca ttcacccttc tctgctgccg aaatatcccg gattacacac ccatcgtcag
     7981 gcgctggaaa atggtgatga agagcacggt acatcggtgc atttcgtcac cgatgaactg
     8041 gacggtggcc cggttatttt acaggcgaaa gtcccggtat ttgctggtga tacggaagat
     8101 gacatcaccg cccgcgtgca aacccaggaa cacgctattt atccactggt gattagctgg
     8161 tttgccgatg gtcgtctgaa aatgcacgaa aacgccgcgt ggctggatgg tcaacgtctg
     8221 ccgccgcagg gctacgctgc cgacgagtaa tgccccgtag ttaaagcgcc agctctgccg
     8281 ctggcgtttt tcaattcacc tgtaaatcgc aagctccagc agtttttttc ccccttttct
     8341 ggcatagttg gacatctgcc aatattgctc gccataatat ccaggcagtg tcccgtgaat
     8401 aaaacggagt aaaagtggta atgggtcagg aaaagctata catcgaaaaa gagctcagtt
     8461 ggttatcgtt caatgaacgc gtgcttcagg aagcggcgga caaatctaac ccgctgattg
     8521 aaaggatgcg tttcctgggg atctattcca ataaccttga tgagttctat aaagtccgct
     8581 tcgctgaact gaagcgacgc atcattatta gcgaagaaca aggctccaac tctcattccc
     8641 gccatttact gggcaaaatt cagtcccggg tgctgaaagc cgatcaggaa ttcgacggcc
     8701 tctacaacga gctgctgctg gagatggcgc gtaaccagat cttcctgatt aatgaacgcc
     8761 agctctccgt caatcaacaa aactggctgc gtcattattt taagcagtat ctgcgtcagc
     8821 acattacgcc gattttaatc aatcctgaca ctgacttagt gcagttcctg aaagatgatt
     8881 acacctatct ggcggtggaa attatccgtg gcgataccat ccgttacgcg ctgctggaga
     8941 tcccatcaga taaagtgccg cgctttgtga atttaccgcc agaagcaccg cgtcgacgca
     9001 agccgatgat tcttctggat aacattctgc gttactgcct tgatgatatt ttcaaaggct
     9061 tctttgatta tgacgcgctg aatgcctatt caatgaagat gacccgcgat gccgaatacg
     9121 atttagtgca tgagatggaa gccagcctga tggagttgat gtcttccagt ctcaagcagc
     9181 gtttaactgc tgagccggtg cgttttgttt atcagcgcga tatgcccaat gcgctggttg
     9241 aagtgttacg cgaaaaactg actatttccc gctacgactc catcgtcccc ggcggtcgtt
     9301 atcataattt taaagacttt attaatttcc ccaatgtcgg caaagccaat ctggtgaaca
     9361 aaccactgcc gcgtttacgc catatttggt ttgataaagc ccagttccgc aatggttttg
     9421 atgccattcg cgaacgcgat gtgttgctct attatcctta tcacaccttt gagcatgtgc
     9481 tggaactgct gcgtcaggct tcgttcgacc cgagcgtact ggcgattaaa attaacattt
     9541 accgtgtggc aaaagattca cgtatcatcg actcgatgat ccacgctgcg cacaacggca
     9601 agaaagtgac cgtggtggtt gagttacagg cgcgtttcga cgaagaagcc aacattcact
     9661 gggcgaagcg cctgaccgaa gcaggcgtgc acgttatctt ctctgcgccg gggctgaaaa
     9721 ttcacgccaa actgttcctg atttcacgta aagaaaacgg tgaagtggtc cgttacgcac
     9781 acatcgggac cgggaacttt aacgaaaaaa ccgcgcgtct ttatactgac tattcgttgc
     9841 tgaccgcaga tgcgcgtatc accaacgaag tacggcgggt atttaacttt attgaaaacc
     9901 cataccgccc ggtgacattt gattatttaa tggtgtcacc gcaaaactct cgccgtctgt
     9961 tatatgaaat ggtagaccgc gaaatcgcca acgcgcagca agggctgccc agtggtatca
    10021 ccctgaagct aaataacctt gtcgataaag gcctggttga tcgtctgtat gtggcctcca
    10081 gctccggcgt accggttaat ctgctggttc gcggaatgtg ttcgctgatc cccaatctgg
    10141 aaggcattag cgacaacatt cgtgccatca gtattgttga ccgttacctt gaacatgacc
    10201 gggtttatat ttttgaaaat ggcggcgata aaaaggtcta cctttcttcc gccgactgga
    10261 tgacgcgcaa tattgattat cgtattgaag tggcgacacc gctgctcgat ccgcgcctga
    10321 agcagcgggt gctggacatc atcgacatat tgttcagcga tacggtcaaa gctcgttata
    10381 tcgataaaga actcagtaat cgctacgttc cccgcggcaa tcgccgcaaa gtacgggcgc
    10441 agttggcgat ttatgactac atcaaatcac tcgaacaacc tgaataaccc tatgccaata
    10501 cacgataaat cccctcgtcc gcaggagttt gctgcggtcg atcttggttc aaacagtttt
    10561 cacatggtca tagcccgtgt ggtagatggt gccatgcaga ttattggccg cctgaaacag
    10621 cgggtgcatc tggcggacgg cctggggcca gataatatgt tgagtgaaga ggcaatgacg
    10681 cgcggtttga actgtctgtc gctgtttgcc gaacggctac aagggttttc tcctgccagc
    10741 gtctgtatag tcggcaccca tacgctgcgt caggcgctga acgccactga ctttctgaaa
    10801 cgcgcggaaa aggtcattcc ctacccgatt gaaattattt ccggtaatga agaagcccgt
    10861 ctgattttta tgggcgtgga acatacccaa ccggagaaag gtcgcaaact ggttattgat
    10921 attggcggcg gatctacgga actggtgatt ggtgaaaatt tcgaaccgat tctcgttgaa
    10981 agccgccgga tgggttgtgt cagctttgcc cagctttact tccctggcgg ggtcatcaat
    11041 aaagagaatt ttcagcgcgc tcgtatggcg gcagcacaaa aactggaaac tttaacctgg
    11101 caattccgta ttcagggctg gaacgtggca atgggcgctt ccggtaccat aaaagccgcc
    11161 catgaagtgt taatggaaat gggcgagaaa gacgggataa ttaccccgga acgtctggaa
    11221 aaactggtaa aagaagtttt acgtcaccgt aatttcgcat cgctgagttt accgggtctt
    11281 tccgaagagc ggaaaacagt cttcgttcct ggactggcga ttttatgcgg tgtgtttgat
    11341 gctttagcca tccgtgaact gcgcctttct gacggggcgt tacgcgaagg cgtactgtac
    11401 gaaatggaag gacgtttccg tcatcaggat gtgcgtagtc gcaccgccag cagcctcgcc
    11461 aaccagtatc acatcgacag cgaacaggcc cgacgggtgc tggataccac tatgcaaatg
    11521 tacgaacagt ggcgggaaca gcaaccgaag ctggcgcatc cgcaactgga ggcgctactg
    11581 cgatgggccg ccatgctgca tgaggtcggg ttgaatatca accacagcgg tttgcatcgc
    11641 cactccgctt atattctgca aaacagtgac ttgccgggtt ttaatcagga acagcagctg
    11701 atgatggcga cactggtgcg ctatcaccgt aaagcgatta aactcgacga tctgccgcgc
    11761 tttaccttgt ttaagaagaa acagttcctg ccactgattc agctattgcg ccttggcgta
    11821 ttactcaaca atcaacgtca ggcaaccacc acaccgccaa cattgacact gattactgat
    11881 gacagtcact ggacactgcg tttcccgcat gactggttta gtcagaatgc gttggtactg
    11941 cttgatctgg aaaaggagca agaatactgg gaaggcgtgg ctggctggcg gttgaaaatt
    12001 gaagaagaaa gtacacctga aatcgccgct taatgctttg cgggccgacg agaaatgtcg
    12061 gcccgcatta ttcaggcact ttcgcgaatg ggttcgattt cattcaacgt atcaattaac
    12121 ggctgcggct taccaataag ataaccctgc atataatcga tccccaaaga gagcaccgcc
    12181 tcgcggatct cttcgttttc aacgtactct gccactaccc gcattttctt cattcgcgcc
    12241 agatggcaaa tcgacgccac aatctgataa tccagactat ttgacacaat attgcggata
    12301 aagctgccgt caattttaag cagatcggca ttcacatttt taagccgtgc atagctggcg
    12361 tagccggtgc cgaaatcatc aatcgcaatc tggcagccta attcctgaag atgctgcaag
    12421 gtgatttgcg cctgcttaac attggtcaga gcattacttt cggtgacttc aaaaataagt
    12481 tgccacgctt caatctgata tttagccagc aactgactga cttcaacagg aaaacgagcc
    12541 tgacataccg aggttggaga cagattaata gcaaaacggt gagcgggcat cttcgctctg
    12601 ttttcagcca taaattgcag cgtatgctca atgacccaca tgtcgatact cgacgataaa
    12661 ccaaattcgt gcgcgaccgg caagaagcta tcggggacga tcagttcatc attctcacct
    12721 ttcatgcgca gaagaatttc atggtaaaca tcaccacgca taccggtaat cggctgggcc
    12781 atcaggaaaa aatggttgtg ttccagcgcc tgctgtagtc gattcatcat cgcgacttta
    12841 tctttcaatt cgcgttgcaa atacattgcc ccgcgacgct gcatattttc cggggcgttg
    12901 gtcacgatgg aaagttcggc gaccgtattt agctctccca gcagcaggta gatatgattc
    12961 actggcgagc gcacatagca gtaactgacg ccaatctgcg gttgcatcgg catcccatcc
    13021 caaaagaaac gaaattgctt gagatgacta tccagtgcgg taatgcgctc ctggtgcgat
    13081 tctgtattca gtcgcagcgc gagatcgtta cccgaaagct gataaacatc ttcacccggt
    13141 tccagcaagg gtgacagcca gtgagaaagt ttttgcttgt attgaatccg cagcatgatg
    13201 ccatagttct taaccagcat ttccatgcca gggatgcgta aataacaaag cgcagaccag
    13261 ggagcatcac gcaacgcgcg attcagggcg cgaacatttg gcagatgaac caccgggtcc
    13321 atatacgcaa gccgctgaat gcgtctgact accgctctct gacgggttgc cagtacagcc
    13381 atataattga caataaaaga gaataccaga taactggagg aggttatggt cagctgcgtg
    13441 gtatagccag gataaatggg aatgtaattt tgatagctgt ggatgctgat catcaacacg
    13501 accgcccaga gcagcgaaat cagcttataa ccatagcgca tcgctcccca catcatcagg
    13561 ggcagcaata atgacaaggt ataattggtg ctaaaaattg tgcttttttc atttaacggc
    13621 atgcataaca gcaacaataa agcacctaat gccagtagcc agagcgcgaa ctcttttttg
    13681 gtgactttgg catcaacctg ctgttttaat tgcgaataat agctacgtaa ataaaatgga
    13741 tttcgcacta cccgaatgat gaagtagcac agcgggacac cgatcagatt acccaccagt
    13801 aaggcctgat aattgattaa agtcccgagg ttaaaaggca tgacaccgac cagattttct
    13861 ctgctcgcca gtaatcctac aaatgcagca aactggaaaa gtatcagaaa cagcgttgca
    13921 ggaaacacaa tctgccagaa tatacgctgg gaaattaaac gggtatcacc atgtgaaacg
    13981 ttgttacgcc ggggagtaaa gaccctgtaa ccgccccaac agagaatgat aatgataata
    14041 aaattagccg ttaatgatag cgtttcgtaa aaacccagct ctgcatattt gcggacaaaa
    14101 atccctaagg cgatccctgg caatgccgcc cagctgaaaa acatcatcat gctgatcatc
    14161 agtgccagag gcagataaaa gagaaaaacc tcaccggaag aaatatgcgc gaaagtatta
    14221 atgtgggcga aaatgggcaa gattaaagaa ggcaggaaca gcggaagccc ccaccattta
    14281 tcacgcattt ttatataagt tgcattcagt ttcatagatg ctcagcagaa tcccccacat
    14341 cctgaaggag gtgtattcag acaggcatcc cacctgactt cgaatgatga ttattcatca
    14401 ctatagagag cattgattct aagtgtcata tgaaagttcc aattgatata tatcaaacaa
    14461 aataaccctg attaatgaat tattacgttt atcatgttaa ttcatcatta ttacatcatc
    14521 attgtaaata attaaattaa cttccataac attaaaatat gtatccactg acgttttttt
    14581 acataacgaa gaattgacca ttttgtcctg ttgtgcctta atgtaagtac cgtccacagc
    14641 gtgggacata cttcaaggaa ccttttgtga gtcaggcaac cagtatgcga aaacgacacc
    14701 gatttaacag tcgcatgacc cgtatcgtac tgctcatcag ctttatcttc ttctttggcc
    14761 gttttatcta ctcgtccgtc ggtgcctggc agcaccatca gagcaaaaaa gaagctcagc
    14821 aatccacact ctccgtcgaa tcaccggtac aacgttagcg gttaccttct tcactttcac
    14881 agaacataac ggcacttcgc tgtcggatgc ttttgctctt tgggattatc aaagcggcag
    14941 atattctttc atcttaaatt ttacgtcttt gtcctgactg atgtttatcc tgtttggctg
    15001 cggaataaat ataaaattaa tatatatgtt gtaatgatat atttttataa attattccct
    15061 gcgtgaattt taataaattt aatctatccc tttatacgca atacatttac tttcctcttt
    15121 tgatgatctt aaatgtctta tttttcgtaa tgtgtataac aaggaatagt gatgaaattt
    15181 aaaaaatgtc ttctgcctgt ggcaatgtta gcgtcattca ctctggcagg atgccagtca
    15241 aatgctgacg atcatgccgc cgatgtttat caaaccgatc aactgaatac caaacaagaa
    15301 actaaaaccg ttaatattat ttccattctt cccgcaaaag ttgccgtaga caactcccaa
    15361 aataaacgga acgcacaagc cttcggcgcg cttattggcg cagtcgctgg cggtgttatc
    15421 ggccacaacg tcggttctgg cagcaattcc ggaacgacgg caggggcagt tggcggcgga
    15481 gctgtaggcg cggcagcggg ttctatggtg aatgataaaa ccttagtgga aggtgtttct
    15541 ttaacatata aggaaggcac caaagtgtat acctccaccc aggtgggtaa agagtgccag
    15601 tttacgacag gtttagccgt tgttattacc acaacgtata acgaaacgcg tattcagcca
    15661 aatactaaat gtcctgaaaa gagctaataa tcaggaggag tcatgaagaa agtttttctt
    15721 tgcgccatct tagcctcctt aagctatccg gctatcgcct catcattgca ggatcaactc
    15781 tctgctgtcg cagaagcgga acagcaaggt aaaaatgaag agcaaaggca gcatgacgaa
    15841 tgggtcgcgg agcgcaacag ggaaatccag caagagaagc aacgtcgcgc aaatgcccag
    15901 gccgccgcta acaaaagagc ggcaacggca gcggcaaata agaaagctcg tcaggataaa
    15961 ctggacgccg aagccactgc ggacaaaaaa cgcgatcaaa gttatgaaga tgagctacgc
    16021 agcttagaga ttcagaaaca aaaactgacg ctggcgaaag aagaagcccg cgttaagcga
    16081 gaaaacgaat ttatcgatca ggaactgaag cacaaagctg cgcaaaccga tgtggtgcaa
    16141 tctgaagctg acgccaacag aaatatgact gaaggcggtc gcgatctgat gaaaagcgtg
    16201 ggcaaagcag aagagaacaa atcggacagc tggtttaatt aatcgatgtt agtaacttca
    16261 agcctatgat tcttgaagat aaaaaaccct ctgtagtaac agagggtttt gttcattcat
    16321 agtgcaggga ttaaaatcat tcccactcaa tggtagctgg cggcttgccg ctgatgtcat
    16381 acaccacgcg ggaaataccg ttcacttcat tgataatgcg gttggaaacg cgaccgagga
    16441 aatcgtacgg cagacgcgcc cagtgtgcgg tcataaagtc gatggtttcg acagcacgca
    16501 gagagacaac ccagtcatac ttacgaccat cgcccattac gccaacggaa cgtaccggca
    16561 ggaacacagt gaacgcctgg ctgactttgt cgtacaggtc cgctttacgc agttcttcaa
    16621 tgaagatggc gtcagcacgg cgcagcaggt cacagtactc tttcttcact tcacccagca
    16681 cacgaacgcc aaggcctggt cccgggaatg ggtgacggta caacatgtcg tacggcaggc
    16741 ccagctccag accaatctta cgcacttcgt ctttgaacag ctctttcagc ggttcaacca
    16801 ggcccatctt catctctttc ggcaggccgc ccacgttgtg gtgagatttg atgacgtgtg
    16861 ctttaccggt tgcagacgcc gcagattcga taacgtcagg gtagatggtg ccctgcgcca
    16921 gccacttcac gtcttccagt ttcagcgctt cttcatcgaa tacttcaacg aaaacgcgac
    16981 cgatgatttt acgttttgct tccggatcgt tttcgccagc cagcgctgac aggaagcgat
    17041 cttctgccgg aacgtgaaca atgttcagac caaagtgatc gccaaacata tccagaacct
    17101 gctctgcttc gttgagacgc agcaggccgt tgtcgacgaa tacgcaagtc aggtttttac
    17161 cgatagcgcg gtgcagtagc attgcggtta cggaggaatc cacgccacca gagaggccga
    17221 ggatgacttt atcgtcgcct acctgctcac ggatgcgggc tacagcatcg tcgataattt
    17281 tcgctggcgt ccacagggct tcacactggc agatatcacg cacaaaacgc tccagcatgc
    17341 gcataccctg gcgggtgtga gtcacttccg ggtggaactg tacgccatag aagcgttttt
    17401 cttcgttagc cataatggca aacgggcagc tttcggtgct ggctacggtg acgaagtcgg
    17461 acgggatagc ggtaacttta tcgccgtggc tcatccagac atcgagcagc ggtttaccgt
    17521 ctgcggtcag cgcatcttcg ataccgcgaa ccagtgcact gtcgtttacg acttcaacct
    17581 gcgcgtagcc aaattcacgt tcgttagagg cttcaacgtg accgcccaac tgcattgcca
    17641 tggtctgcat gccatagcaa acgccgaata ccggtacgcc tgcttcaaag acatactgcg
    17701 gcgcacgcgg gctgttttct tcagtagtac tttccgggcc gccggaaaga ataatgccgc
    17761 ttggattgaa gtcacgaatt tgtgcttctg tcacatccca cgcccacagt tcgcagtaaa
    17821 cacccagctc acgcacgcgg cgcgcaacca gttgagtgta ctgagaaccg aagtctagaa
    17881 tgaggatgcg atgcttatga atgttttccg tcattgacgc ttattccgag gcaagtgaaa
    17941 cagataatat aaatcgcccg acatgaagtc gggcgaagag aatcaggagc ccagacggta
    18001 gttcggggac tctttagtaa tggtcacgtc gtgaacgtgg ctttcctgaa tgcccgcacc
    18061 gctgatacgt acaaactccg ctttagtacg cagttcgtcg atagtaccac agccggtcag
    18121 acccatacag gagcgcaggc cgcccatctg ctggtgaatg atctctttca ggcgaccttt
    18181 ataagctacg cgaccttcga taccttccgg caccagtttg tcggcagcgt tatcgctctg
    18241 gaaataacgg tcagaggaac ctttggacat cgcgcccagg gaacccatac cacggtaaga
    18301 tttgtaagaa cggccctggt agagttcgat ttcacccgga gattcttcag tacccgccag
    18361 catggaacct accatcaccg cgcttgcgcc agcggcgata gctttggcga tgtcgccgga
    18421 gaagcgaata ccaccatctg cgataaccgg aataccggtg ccttccaggg cttcaactgc
    18481 gtcagcaacg gcagtgatct gcggaacacc tacgccagta acgatacgag tcgtacagat
    18541 agaaccaggg ccgataccga ctttaaccgc gctgcaacca gcttctgcca aagcgcgtgc
    18601 acctgcagct gttgccacgt tgccgccgat gatttgcaga tccggatatt tagcacgggt
    18661 ttcacggata cgctgcaaaa cgccttctga gtgaccgtgg gaggagtcga tcagcagaac
    18721 gtcaacgcca gcggcaacca gcgcgtcaac acgctcttcg ttacccgcac ctgcgccaac
    18781 tgcagcacca acacgcagac ggccttgctc gtctttacag gcgttcggtt tacgttccgc
    18841 tttctggaag tctttcacgg tgatcatgcc gatcaggtgg aattcgtcat caaccaccag
    18901 cgctttttca acgcgttttt cgtgcatttt tgccagcacc acttcacggg cttcaccttc
    18961 acgcacggtg accagacgct ctttcggcgt catgtaaacg ctaaccggct ggttcaggtc
    19021 ggtaacaaaa cgcacgtcac gaccggtgat aatacccacc agttcgtttt cttcggtaac
    19081 gaccggatag cccgcaaaac cgttacgctc ggtcagttct ttcacttcgc gcagcgtcgt
    19141 ggttggcaga acagtctgcg gatcagtcac cacaccagat tcgtgttttt tcacacggcg
    19201 aacttcttct gcctggcgtt caatggacat gtttttgtgg ataaagccga taccgccttc
    19261 ctgagccaga gcaatagcca ggcgcgcttc cgttacggta tccattgctg cggaaagcat
    19321 agggatattc agacgaatag ttttcgtcag ctgggtgctg aggtcagcag tattcggcag
    19381 aacggtagaa tgagcaggaa cgaggagaac gtcgtcaaac gtcagagctt ctttagcgat
    19441 acgtagcatg ggcaatatct cgaccagagt ggttaataaa tattgccgcg gcattataca
    19501 gagcgtaacc gattgcatct accccttttt gcaaaaaatg cttgctatcc ccgaagggcg
    19561 ggttactatc gactgaataa cctgctgatt tagaatttga tctcgctcac atgttacctt
    19621 ctcaatcccc tgcaattttt accgttagtc gcctgaatca aacggttcgt ctgctgcttg
    19681 agcatgagat gggacaggtt tggatcagcg gcgaaatctc taatttcaca caaccggctt
    19741 ccggtcactg gtactttaca ctcaaagacg acaccgccca ggtacgctgc gcgatgttcc
    19801 gcaacagcaa ccgccgggtg accttccgtc cacagcatgg acaacaagtt ttagttcgcg
    19861 ccaatattac gctctacgag ccgcgcggtg actaccagat aatcgttgag agtatgcagc
    19921 cggccggtga agggctgctg caacagaagt acgaacagct caaagcgaag ttgcaggctg
    19981 aaggtttgtt cgatcagcaa tacaaaaaac cacttccctc ccctgcgcat tgcgttggtg
    20041 tgatcacctc aaaaaccggt gctgcgctac atgatatttt gcatgtgtta aaacgtcgcg
    20101 atccttctct accggtgatc atctacccca ccgccgttca gggcgatgac gcaccggggc
    20161 aaattgttcg cgccattgaa ctggcgaatc agcgcaatga gtgcgacgtg ttgatcgttg
    20221 ggcgcggcgg cggttcgctg gaagatttat ggagttttaa cgacgaacgc gtagcgcggg
    20281 cgatttttgc cagccgcatt ccggtcgtca gcgccgtcgg gcatgagacg gatgtgacca
    20341 ttgccgattt tgttgccgat ctgcgtgcgc caacgccgtc tgccgccgct gaagtagtga
    20401 gccgtaatca gcaagagtta ctgcgccagg tgcaatcgac ccgtcaacgg ctggagatgg
    20461 cgatggatta ttatctcgcc aaccgcacgc gtcgctttac gcagatccat caccgattac
    20521 agcaacagca tccgcagctc cggctggcac gccagcaaac catgcttgag cgcctgcaaa
    20581 agcggatgag ctttgcgctg gaaaatcaac ttaagcgtac cgggcaacag cagcagcggt
    20641 taacacagcg gctgaatcag caaaatccac agccgaagat tcatcgcgcg caaacgcgca
    20701 ttcagcaact ggaatatcgt ttagcagaaa ccctgcgcgt acagcttagc gccacgcgtg
    20761 aacgtttcgg taatgcagta acgcacctcg aagccgtaag cccactgtca acgctggcgc
    20821 gtggatacag cgttactact gctactgacg gcaatgtact gaaaaaagtg aagcaagtta
    20881 aagcgggtga aatgctaacc acacgtctgg aagacggctg gatagaaagt gaagttaaaa
    20941 acatccagcc agtaaaaaaa tcgcgtaaaa aggtgcatta agccagcacg aattcaacgc
    21001 gttttttcga tatcaagcca tgcccgtgct gacagaagta atctactgca ccgcaggctt
    21061 ttaacacctg caacggttga tggcagtctg gacaaagagc tttcatttca ataatttccc
    21121 cgcaggatgg gcaacgcgca tggccgttat cctgatcaag cacatgctga cattgtggac
    21181 aatgcagttc catatgaatt cctccggtag ccatccctct acattcatag agggaatggc
    21241 agataaaata cttacggata attatttatt tttcttgatg tgcttcatca gacgcttacg
    21301 tttacgcatc tgggttggcg tcagggtgtt acgcttgttc gcatacgggt tttccccttc
    21361 tttgaactga atacgaatcg gcgatcccat tacgtccagc gatttgcgga agtagttcat
    21421 caagtagcgc ttgtaggaat caggcaggtc tttcacctga ttaccgtgaa tcaccacaat
    21481 cggcgggtta taaccaccag cgtgggcata tttcagcttc acacgacgac cgcgtaccag
    21541 cggcggttgg tgatcttcaa cagccatcgt catgatgcgc gtcagcatag aggtccccac
    21601 acgacgggtg gagctgtcat acgcttcacg tactgattca aacaagttac caacaccact
    21661 gccgtgcaag gcagagataa agtgcacacg agcaaaatcg ataaagccca gacggaagtc
    21721 cagcgtttct ttcacctgct ctttcacttc ctggctcagg ccatcccact tattcaccac
    21781 aatgacaagt gagcgcccac tattgagaat aaagcccagc agcgagagat cctgatcgga
    21841 aataccttcg cgcgcatcaa tcactaacat caccacgttg gtatcttcaa ttgcctgcaa
    21901 cgttttgatt acggagaatt tctctacagc atcggtgatt ttgccgcgtt tacgtacgcc
    21961 agcggtgtca atgagcacat actcacgtcc atcgcgttcc attggaatgt agatactgtc
    22021 acgcgtcgtg ccaggcatgt cataaacaac aacgcgctct tcaccaagaa tacggttagt
    22081 gagtgtagac ttacctacgt tcggacgacc cacaatcgcc agtttgatcg gcagactttg
    22141 cgggtcgaag tcgtcttcct cttcttcttc gccgttctct tccgcttcaa attgcgccca
    22201 gtattcagcg tcttcgtcga cttcctcttg cggtgcgaga tcttccatcc acggcagcag
    22261 cacacgctcc agcagactta atacgccacg accgtgagat gcggcgatcg ggtagatttc
    22321 acctaaacca agcgagtaga aatcaaccac tgcctgatcg ggatccagac cgtcagtttt
    22381 gtttgccacc aggaaggtcg gtttttcacg agagcgcaga tgtttggcaa tcgcttcatc
    22441 tgccggcatc aagcccgcgc gcgcatccac cataaacagt acgacgtccg cttcttcaat
    22501 cgccagcagc gactgttccg ccatgcgggt ttctacaccg tcttctgtgc catcaatccc
    22561 gccggtatca atacagataa actcacggcc ttcaatttcc gcacgaccgt acttacggtc
    22621 acgagtcaga cccgggaaat ccgcaaccag cgcatctcgg gtgcgagtta gacggttaaa
    22681 taacgtggat tttcctacgt tagggcgccc gacaagcgcg accacaggta ccatgtttaa
    22741 agcctcattt ttataaatca tcagacaacg cacgctatat tcgcgtcgtt gttaaaaaca
    22801 ggaaaacggc ccctgtccag gagccgtttt caaagtgaac gacagagacg attaacgtgt
    22861 aatagagtac acggttccgt cttttgcctg gatcagcagt ttgccgtcag cggcaaccgg
    22921 ttcagtctgg aaaccggaac tatcaacttt ttgctgggca acgaaacgac catcttcgac
    22981 gttaatccag tgcagataac cttcactgtc acctaccacc aggttgccat tatacagcac
    23041 cggcgaagtc agcaggcgat gcagcagatc gctttgtgtc cacagcgtaa cgccgccatc
    23101 aatggtcaac gccatcaccc ggtcattttg atcgaccaga tagatgcgat tgccgtcgac
    23161 gatgaaatca ttcaccgaac ccagttcgcg tttccacata atctgaccac tgcgcagatc
    23221 aagcgccgtc aggttaccat tataggccag agcgaaaaca acgccgttaa caacgacggg
    23281 agtcgtgtca acatcgctca gacggtcaat ttcggtagaa ccggtcgcct gggaaatacg
    23341 ctgctgccaa atcatctggc cctgttccat cagcactgcg ctgacgcgac cattatcgcc
    23401 ccccacgacg gccgcaccaa aagccgttgc cggcgcagac tcgccacgca aagagagcga
    23461 aggcatatcg aggttaactg tccatttgac agcgccgtca gcttcgttca gcgcttgtaa
    23521 ctgaccgtta ctagtgtgga ttaacaccag accgtcgctg accaccgggc gagaaagtgc
    23581 ttcacccgcg actttagttt gccatgccac agtaccatcg ctggtattca gcgcgtaaac
    23641 ctgcgccttt tcgctgccaa tgtagacatg cccaccagac acggtcacac cgccagaaag
    23701 taatgcaggc tctttagaga accagccatc tttctcggcc aggctgacag accagatttc
    23761 tttgccatca tccgcattca gcgcttttac caaaccagcg cggtccgctg catagacaac
    23821 gttgtccgcc agtgccggat gaagattgga atagaagttg ccaatgccgc taccaacgga
    23881 agtgctccac gccgtggtcg gcgtaaactg gttttcaacg gttggcaatg gggacatctt
    23941 taccacatct tcttcgctgt taaacagcga acagccgctt aaaagggtaa cggaaagcag
    24001 tcctggcagc agtaatttac gcaattgcat cgggtccctc tcagatggac aaattattaa
    24061 ttttcatctg catcatttcg ctcagtgccg gagtaacatc gcttttcacg cctgcttccc
    24121 atgcactacg cgcaccttgc ttatcacctt tgctcaacaa tgctttacca cgcaggtcgg
    24181 caacaatggc agcccaccct tcacctttga tggtatcaag ggttttcagc gcggcatcag
    24241 cctgcttgag ctgtacctga acgcgagcaa gacgcagatt tatcacggct ttgagatttt
    24301 catcactcgt gtctgccagc ccctgttgta actgggcggc agctttctct agttcatttt
    24361 tgtcaacaaa ttgctgcgca agttccaaag aagccagcgc accataagta tttttatttt
    24421 cagcagcaaa tttttccgcc gccgggatgc tatccggttt gccttcgctc actgcggtaa
    24481 ccgcattttg ataggcaaga gaagcggagc gtgcagaatc aacctgatgg ctgttccagt
    24541 agcgccagcc aatcagtgcg ccaacgccca aaatcacccc aacagccagt gctttgccat
    24601 tttcagcaaa aaagcgttta accgcttcta cctggtcgtt ttcgttctcg taaatttcca
    24661 cgctgtcctt ctccttcctt aacccagtaa cgtgcgcaaa tgcgcggcta cgctatcctg
    24721 cgcaactgcc gtttgctcac cagagcgcaa atccttcact actgctgtgc cgttagccac
    24781 ttcagactca cccagcacca cagcaacgcg ggcaccccat ttatcagcac gggcaaactg
    24841 tttcttaaag ttgccgccgc cgtggttggt catcaatttc acgcccggta attcatcacg
    24901 cagacgctca gctaatgcca tagccgcaga ttgtgtatca gcacctgaag ccaccaggta
    24961 tatatcgaca acaggatcgg ctttaaattc cggattaacg gcctgtacta acaatacaag
    25021 acgttcgagg cccatcgcaa aaccgactgc cggtgttgca cgaccgccca gttgttccac
    25081 cagaccgtca taacgaccgc ctgcacacac ggtgccctgg gagccgaggc tgttagtcac
    25141 ccactcgaaa acggtacggt tatagtaatc cagaccacgc accagacgct ggtttacggt
    25201 gtaagcgatc cccgcgctct caagcagttt gcacagaccg gcaaaatgct cacgagattc
    25261 ctcgtccaga taatcaccta atgccggagc gtcgttgaga agcgcctgca cttccggatt
    25321 ttttgaatcc agcacgcgca gcgggttagt gtacatgcgg cgtttgcagt cttcgtccag
    25381 cttttcttta tgctgctcaa ggaatgccac cagcgcatcg cggtaattgg cgcgtgcttc
    25441 cagcgaacca atagagttca gctcaagagt tacgtgttcg aagataccca gcgcacgcca
    25501 ccagcgggca gtgagcataa tcagttcagc gtcgatatcc ggaccttgca gaccgaaaac
    25561 ttcgcagccc aactgatgga actgacgata acgccctttc tgcggacgct cgtgacggaa
    25621 catcggcccg atataccaca gacgctgttc ctgattgtac agaagaccat gctcgatgcc
    25681 ggcgcgtaca cagcccgccg tcccttcagg gcgcagagtc aggctgtcgc cattgcgatc
    25741 ctcaaaggtg tacatctctt tttcaaccac gtcggtgact tcaccaatcg cacgtttgaa
    25801 tagcggggtc tgctctacaa tcggcaagcg gatttcactg taaccgtagc tgccgagcac
    25861 gtttttcagt gtgccttcaa tgcgctgcca gatggccgtt tcgccaggca ggtaatcgtt
    25921 catgccgcga atggcttgaa tgttttttgc cacgtttatt ctctttctga atataaaaat
    25981 gaaccctcaa cgcttccctc aatgtttcgg gagccatgcg ggttcaatca tacacgggaa
    26041 gcgaggcgct tcccatcacg ttattatttt tcaacctgct gaacgtcaat tcgacgcgct
    26101 tcgtccagct gactggcttt cgcacgaatg cgtgcttcca gctggtcgat catatcgttg
    26161 ttgtccagac ggtctttgcg cacgccatct tcatagaggc cgcttttctt gttgccgccg
    26221 gtgacgccga gtgtagaaac cagcgcctca cctgggccat tcaccacgca gccgataatc
    26281 gaaacgtcca tcggagtgat gatatcttcc aggcgttgct ccagcgcgtt aaccgtaccg
    26341 ataacatcaa attcctgacg cgaacaggtc gggcaggcga tgaagttaat ccctcgcgaa
    26401 cggatacgca gcgatttcaa aatatcgaaa ccgactttga tctcttcgac cggatcggcc
    26461 gccagcgata cgcgcagcgt gtcgccgatg ccttccgaca gcagcagacc taaaccaatg
    26521 gcggatttta ctgccccgct gcgtgcacca ccggcttcgg tgatccccag atgcagcggc
    26581 tgatcgatct gttttgccag caaacgataa gactcaacag cgaggaagac gtcagacgct
    26641 ttcacgctga ctttgaactg atcgaagttc aggcgatcga gatgatcaac atgacgcatg
    26701 gcggattcca gcaacgcctg cggcgtcggt tcgccatact tttcttgcag atctttttcc
    26761 agcgatccgg cgttaacgcc aatacggatc ggaatgtttt tatcgcgcgc acagtcaacc
    26821 accatgcgaa tacgctcttc attaccgata ttgccagggt taatacgcag acaatcgacg
    26881 ccgtattccg ctactttcag cgcaatgcga tagtcgaagt ggatgtcagc caccagcggc
    26941 acgttaaccc gctgtttgat gagtttgaac gcttctgccg cgtccatcgt cggtacggag
    27001 acacggacga tatcagcgcc aacgcgttcc agcgccttga tttgattgac cgttgcttcg
    27061 acatctgtcg tacgcgtatt ggtcatggac tgtacggcga tgggagcacc atcgccaatc
    27121 ggcacattcc caacgtaaat acgtgttgat tttctacgtt gaattggagc ctggttatgc
    27181 atgaaaaatc tcccgcgtta cccgtctgtt actgcgccgg tgattgttcg gcattgaggg
    27241 tcagacgcgc aacctggtta gttctgataa aacgactcag atcgacaggt ttcccttgat
    27301 actggatctg tactgcggct ggcgcaccaa ttttcagttt atacggtgcc tggccggtta
    27361 aattcaaatt accgtcttta cgctgcatcc cgctaaacaa ttttttaccg gtagcatcag
    27421 tgacctccag ccagcaatcg gcagtaaagt tcatcaccag cgcattcgga tcagccgccg
    27481 gcgtggtcac gccagcctga tcggttggca acggcgcagc accatctggc gttgttgctg
    27541 ccgttggtac cggggtcgcg gcggtatcaa catttgcctg cgaaggcgaa acaaccgcat
    27601 tctgttgcgg atcaacagct ggtgctggcg cagttacggc aggtgtttgc gtgttggttg
    27661 cggtagtatc cacagacgct ggcggcgtgg acgttgtagc cgggtctgta gttgtcgacg
    27721 tatttaacgg gacactctgc ccctgctcgc tattactgct cagttctgcc gaagattgat
    27781 cggccatagt ggtgatctct tcctgctgag ctttgtggtc ttgccaccac caggcaccgc
    27841 tcaggccgat aaccacaaac aacaccagcc aggtgaaggt catcagccag ccatcacgtt
    27901 ttttgcggcg tttaccgagg gaaaaactct gcattggcgc aacttttgca gcccgtagtg
    27961 gagcctgctt ttccagccct ggcagcagtt cttcttctgg aatatgtacc agacgcgcat
    28021 aagagcggat atatccgcgc aggaatgttg aagcaagatc ggcgggtgcc ttatcttctt
    28081 caatgtcgcg taccgtggaa accttcaggc aaagtcgctc ggcaacggcc tgctgactaa
    28141 gtcctagttg ttcgcgagca ttacgcaggc gagcgccggt agtaagtgct tcattttggt
    28201 cgtgcgtggc ttcagtattc attcgctgca ggtacgttta aatgagaatt gagatgccgg
    28261 tgaaccatca tgctcaccca caccgcgaaa catccgttaa gttaaccgtt atcatacagt
    28321 ataagaccgt ctgtgtgctc gtgacaaagc ccacattcac taaggctaac ttactgttgc
    28381 atcgttacat actgccttaa agtcagcaaa aacgcaccgt taatattgac cagacaattg
    28441 caacttaata cttcattcat aaaacattac gccacggtac ataaagtaac cgtggcgtaa
    28501 tggctatcag accgctttaa tgtcgatggc ttcaccctgc atccgtttac gcagggtacg
    28561 tttcgtacgg tcgataacat cgcccgccaa ctgaccacag gcagcatcga tatcatcacc
    28621 acgagtttta cgcacaatag tggtgaaacc gtagctcatc agcacttttg agaaacggtc
    28681 gatacggctg ttcgagctgc gtccatacgg cgcacccggg aacgggttcc acgggatcag
    28741 gttgatctta cacggcgtat ctttcagcag ttccgccagt tggtgcgcgt gttcagtgcc
    28801 gtcgttaacg tggtcaagca tcacgtattc aatagtgact cggccctgat tggcgttgga
    28861 tttctccaga taacggcgca ccgcagcaag gaacgtttcg atattgtact ttttgttgat
    28921 cggcacaatt tcgtcacgaa tttcgtcgtt cggcgcgtgc agggaaattg ccagtgcaac
    28981 gtcgatcata tcgcccagtt tatccagcgc cggaactaca ccggaagtag aaagcgtgac
    29041 gcgacgttta gacaggccaa aaccgaaatc atcaagcatg atttccatcg ccggaacgac
    29101 gttgttcagg ttgagcagcg gctcgcccat gcccatcatc actacgttag tgattggccg
    29161 ctgaccggtg acttttgctg cgccgacgat tttcgccgcc cgccacacct ggccgataat
    29221 ttccgacacc cgcaggttgc ggttaaagcc ctgctgggcg gtggaacaga atttacactc
    29281 cagcgcacac cccacctgcg aagagacgca gagcgtggca cggtcgtctt ccgggatata
    29341 caccgtttcg acgcgctgat cgccaacggc gatcgcccat ttaatggtgc cgtcggatga
    29401 acgctgttct tcaaccactt ccggtgcgcg gatctccgcc acctctttca gtttgccgcg
    29461 caacactttg ttgatgtcgg tcatctcatc aaagttgtcg cagcaatagt gatacatcca
    29521 cttcatcacc tgatcggcgc ggaagggttt ttcacctaaa tctttaaaaa actcccgcat
    29581 ctgctgacgg ttgagatcca gcaggttgat ttttccatct ttcgtggtga cgttttcagg
    29641 tgtgactaat tgttcagaca tatgctattc cggcctcgtt attacacgtt atggcccctg
    29701 gagggttgaa aaaagaaacg ccccggtgag cggctgctcg tccgggggcg ctgcattgta
    29761 caaattctgg cgcacggatg ccacgtttgc acgcggcatt tacgaaatta ttaacgggtg
    29821 cgcgggcaca cttcgccttc gccgaagaaa taagcgattt cgcgagcggc agattcgacg
    29881 gaatcagaac cgtgggtacc gttttcggtc aggctgtcag cgtaatcagc gcgcagagta
    29941 ccggccagtg cgtttgccgg attggtcgcg cccagcagat cgcggtgacg ctgaacggcg
    30001 ttttcacctt ccagcacgga aaccacgatc gggccagagg tcatgaattc aaccagacca
    30061 tcaaagaacg gttttccatc gtgttcagca taaaagccac gagcctgttc aacggtcagg
    30121 tgcagcattt tggtgccgac aattttgaac cctgcagctt caaagcgcgc aaagatatta
    30181 ccaatgacgt tttttgctac cgcgttcggt ttgatgatgg aaaaagtacg ttcaatagcc
    30241 atttttacct ctgtaaattg ttctgttgtt gtctgtacca gcgtacgaga tggcgcggat
    30301 tataatgagc aacagggccg ttgactattg atgaaggtaa cattttttta aaataaaatg
    30361 agttttagca acaatcaggg acaaaacaga ctattgcatg acaaatttca ctgtcgcgat
    30421 ttgccccacg tcatccatca ccagcaattg ataatcacct ttatccgtca aatgcagggt
    30481 cacgttgcgc ccgcgttcag ttaacggttc gccattcaga aaccaccagc gttcacctgc
    30541 ccctccactt gattgcaacg gcaaagtcgc ttccgctgcg cccggtaaac gtttaataat
    30601 cgcgccatcg cgaacgcctg tcagctgcag agggagctgg gcatcgtgac cgtatggcgg
    30661 gcaaattgtc gaggccggtg gtaagcgcac agcgcgacgt tctgatgcgg gcagccaggg
    30721 ttccagcggt agcggccaga catttaccat ttcttgcttc gcctgcgggc agtcagcggc
    30781 gacgcgctgc cctttgtcat ccagccagat ggggaagcga atgccattaa tcccttcctg
    30841 ctctgacagt aacagcgttg gcggctggct gccatccagc agccaggttg ccaggcggcg
    30901 gcgacagtta ccgtcacctt ccggcaaaga ctgtccgccc ggccagcaga taacgccacg
    30961 agtgactgag ttcgggcgcg ggtcttccgg cagattcgca ctgcgcgaca gtaagatatt
    31021 attgacctga ttcaacaatg gtacggcact ggcaaagcca aactgaccaa caacgggcgt
    31081 gccgtcaggt ctgccagtcc agatcccaat gacatagcga gcgttaaccc caatcgccca
    31141 ggcgtcacga tagccatagc tggtgcccgt tttccatgcc agtggggcga cgcgcggcaa
    31201 ggcactatcc ggcaagggtt gcgcttcatc agccataatc cggcgaatga tccacgccgc
    31261 ccccgacgac attaaaggcc gttcaagcag cggatcgtca ggctgtaagc gcaatttgcc
    31321 tgccttgccg tggcgagcaa acgcggtata cgctgccgcc atatcttcca gttttgcacc
    31381 agcgccgccg agaatgagtg aaagattcgg cgcagcgccg ttgggcaaat ataacggcaa
    31441 tccaacattg cgtaactttg ccgcaaaccg tttcggtcca taggcttcca gcacctgcac
    31501 agcaggtaag ttcagcgagc gcaccagcgc ctcgctcatg ctgatcgggc catgaaaacc
    31561 gctatcaaag ttacctggtc gataatcacc ggtgcgccgg gggacgtctt gcagcagtga
    31621 tgccgggtgg atcaagcctt catccagcgc cagaccataa acaaacggtt tgagcaccga
    31681 tcctggcgat cggatcgcat tgaccatatc aacatgacca aagcgtgaat catcgttgag
    31741 atcaaccgat cccacccagc cgcgaacacg catatcggta tgatcaacca cgatcatcgc
    31801 cagtgagctg cgcggtggca accgcccttt ccagttttgc gccagttctt ccagacgtcg
    31861 ttgaagaccg gcatccagcg tagtaacgat tttgtcgctt ttgcttttac cgagcatcat
    31921 gcgcgaaaac agcggtgcca gttgcggcat ttgtcggggt gccagccaga tgggttcttc
    31981 ccttgactct tttacctgct cacgggacca cacaccttgc gcagccatcc gttcgagcac
    32041 tttattacgc gcggcttcgg cacgctccgg ccaacgatcc gggcgaagac ggctgggcgc
    32101 ttgcggcaaa accgccagca ttgccgcctc ggaatagctt aaattcgcag gcgactttcc
    32161 gagataagcc caacttgccg caccgatccc ctgcaacgta ccgccaaacg gagcgcggtt
    32221 aagatacaag gtcagaattt cacgcttaga cagatgccat tccagttgca acgcgcgcca
    32281 gagctggcga attttgccgc caaatgtttt ggggtgagga tcaagcagac gagcaacctg
    32341 catagtgagc gtgctgccac cggaaataac ccgtcccgaa gtgagatctt gccatgctgc
    32401 gcgcgccacc gagaatggat tcacccccgg atgcttccag aaccagcgat cttcataatt
    32461 gatcagcgct tcaaggtaac gtggagaaac atcttcgatt gttaccggat aacgccagat
    32521 gccgtcagca tcggcgaagc gccagagcgg cgtaccatcc tgcgccacga ccactcgtgc
    32581 gggattgact tcatgcagcg gtagaggcca gagtttatct gctccccacg ctgcaagaaa
    32641 aaggagaaat ggcgcagctg ccagcgttat ccagcagccg cgtttggtta acaagcgagg
    32701 catttacggt cggacaatca gcagatcttc agccgcgccg gtcgcccgcc attggggaac
    32761 atacattgat tccaccatcg gttgcggtac ctgatacgtt cccggcgtca ccgcccgcgc
    32821 cagatacacc agcgttaccg gttggtattc atcaacggca accgccgcca caaagcgatc
    32881 gtcacggaac tcaatgtgct taatactcgc ctgctgcatc tggttcagta agttctgcac
    32941 ttcgccacca ctttgctcca ggctggcgct accgttcgcc agattctggt tttccagttc
    33001 cagacccaca ggcagcagat ccacgactaa cgcatccggc acgctgttac tggcttttac
    33061 ctgcaaccac accagcacca gatcgccgct acgtaacgag tccagcgatt tgctcttacc
    33121 atcagtacca agaatatgac gctcgatttg cagcacattg ttcgccggta aaggtgcgga
    33181 ttgcggataa ccgctggcat ccatacgcaa ccataacggc tgatcgccac tgttgctcac
    33241 ctgcaaggtg acaagttgat cgctattcag attgctgttt tgcgctttct cgcctgtcag
    33301 cggctcagct gagaaagagg tttgcgcctg ccatttaccc ggtaaatcct gaatcgtacg
    33361 ggcagccagg aacaacgcgt tactttcctg cgtcgatagc cagcgttcac caaacgcctg
    33421 ctgcgaaagt gtgttcagca aagtgtactg ctcatcgggt agcagtttat tttcttccag
    33481 caaggagagc attaacgcgt tgtcgcgcag tggactaccg taatcaccca gccatatccg
    33541 ctcatcacta tttctcggcg ttttcagcgc cagcgcaatc gcttcttcac cacgcgtcgc
    33601 atcacccatg gttttcagcg caatgccaag ttgcagcagc ggtaaaccag aagcggcatc
    33661 tgcacgatgc tcccagattt cacgcagcgc acccagtggt gctttttgct gacgggccag
    33721 caccagcgca gcgtaagact gaacggcgaa tttactggct ttgagattat ccgcgtacgg
    33781 gatcgacatc atgcccggat cttgtaaata gcgcagcaga cgctcattac cccggttaat
    33841 ggcgtctgtc ggcacgctgt aaccctgttc gcctgcgcgg accaggaaat ccatcacgta
    33901 agccgtcagc cagtactctt cgtcaccgtt tttatcccac agcgcaaagc cgccgttatc
    33961 acgttgcatt tgcagcaaac gggaaatgcc gatatcgacc gatgcacggc gtttctcatc
    34021 actgtcgcct ttgatgccca acgcctgcag ttgggctgcg ttggtataaa gtgacgggaa
    34081 caggccgctg gcggtttgct caagacagcc gtacggatac gcttttaact ctttgatata
    34141 acgtgcaatg ttcagcggtg gtttgccgct caacaacaat tgcccttcca gcgtaacagg
    34201 cgagaagttt tgcaatccat ccgccggaat cgcccatgtc tcaccaggct gtaacgccgt
    34261 accgtaatta accgtttgtg ccgggaacgc cggacggacg ccgattttcc actgcttatg
    34321 ctgatcggca acggtttcac ccggtaacgc taacccgcta atggtggcct gaatttctcc
    34381 atcgccataa cccggcaatg ctcgcaccgg gataaacagc gtagtacgca cacctggcgc
    34441 taattcaacg gctgcgggtg aatcgctgac cagttcaagc aaaccactgg cggtcagggc
    34501 aacgttcagt ttttgcggtt tatcggtaag attagtgata tccagcgtca gacgcgaggt
    34561 atcgccactc gccataaagc gcggcatgtt cagttcagca atcaccggtg cggcaactat
    34621 cactttgctt tcgttgctac cgaaatcatc tgccgtccag gcttgcgcca tgacgcgcaa
    34681 ttcaccgtta aaatcgccaa tcggcagtgt aaccgagcct tcgccctgtt cgttgagcgt
    34741 taccgacagc gcctgctgcg cgacaatatt gacgtgattg accggcggtt taccaccacg
    34801 tttcagctca tcaccatcgc caccgaaacg cagagctgcc agacgcccct gaccttcaat
    34861 aacctgaccg taaatatcgt aaatgtctgc gccatagcgt ttctgaccaa agaacgcctg
    34921 ccacggatct ggcgtaacgt agtcagtaat attcagcaca ccactatcaa cggcagacac
    34981 cagcacattc acctgtttag gcttctcgcc atttttagtg ctggctttaa ttttcacggt
    35041 taatggttga ttgggacgca tttttgctgg tgtttccagc gccagatcga ggcgacggtt
    35101 ttcatcacca agcggcagat gcaacacacc aaccgcgcgt tttggcgtcg cggagcgaga
    35161 tttatcgcca ggacgcacca ccagcgtact caaataaaga tcatgacgat tccaggtttt
    35221 atcgaccgga atcgtcagat ccagcccttg agccggaaca tcaatctctt gccaccacag
    35281 cggcccttca ctggactcga ccatcgcata acctttaccc gccgttggcg cggcaatatg
    35341 caacttaatg gtatcgccgg ggcgataact ggctttatct agtttcagcg tgacacggtc
    35401 gggtcgcact gcgccgctcc cgtcgctgtt gtcctgccag ctatagccag cccaaaaacg
    35461 cacgctactg accgcttcat tcggcgcttt gacttccaga cgataagcgc cccactctac
    35521 cgggaaactg accttaccgg tttcatccgc ctgaagatcc agagtttgtt cattttcgat
    35581 cagatctttt tgatcaaact gtgactgcca gccttcatct tctgaccagt tccagtagta
    35641 atcgcggcgt tcgcgaatca ggcgcacctg caagcccgac acggcttttt tcacgccttg
    35701 cgcatcgcta taaacgatgt caaaagcggc gttactgcct tcatcaacaa tgggctgttt
    35761 caccgtgctg tcagtgcgat aatcgtaaac cgatttcaag gcgaactgcg gacggagccc
    35821 cggcaatgca tcggcaggcc agatagcctg ctcagcgcgg cgcgtcaccg ggcgaccgcc
    35881 cgattccagc aaactgccct ggaaaataac ctgcaatggg gaatgcgttt ccttccactg
    35941 gctttctgta gaaacttcgc cgcgcccttt atcatccagc gtcaactgaa cttcatccag
    36001 cgtgcgggaa aggttttcgg cagctatatc gccgaattca aaaccaggta aggctgacac
    36061 agcctcacgc agtgggcgca ggaaaagttg cccttgcaaa gtattaccat tagcaggcgc
    36121 accgtacagg tagtatccca ccacggagaa tttcacttca tcattcggcg taagcggggt
    36181 tttctcaccg gtcagattca gcgccatacg ctctggcata aaatcttcga cgtggaaatc
    36241 ccacatccgg tactggttat cgcccgtgtt ggcgcgaata tgccacatac cggttgccgc
    36301 attgctatcg agtggccagg taaagtggta gaggccattc tccggctgac taacgacgtt
    36361 cctgagtacc tgcccgtcag gtttaatcac gtctaacttg atgggttgat cgggcaatgc
    36421 tttaccgtct gcatcgcgca gtaaaccatt gaggattacc gtttcacctg ggcgataaag
    36481 atcgcgtggg ccaaacatga aaaactgttt gctatagcct ggcgcgccag caatgttaaa
    36541 ttctgctaag tccagcgccg gaagttttaa atcgagtagc gttgtctgac cgtctttacg
    36601 tgccagcagt aatgccgcat ttttatcatt ttccagctgc acatgcccct gagcgtcact
    36661 ggttgcctga gtcagagtct gtcctttctc atttaataaa gagacttcaa ttccttgctg
    36721 ggccgcgccg ttttccagac tttgggtaaa gatatccaga cgattgtgat aacggtgagc
    36781 tgaaacgccg atatcactta acgtaaacag cgtcgcggga ttactgtaat cgtaacgtcc
    36841 agcctgattc atcacagcca gatacacgcc cgcctgctga agcggtttga tatcgcccag
    36901 cggcagcaat aatttttcac gggtgttacg cgcaggattg agatcaaacc gtccggtgta
    36961 gaccagatcc gccatctgca gcagtttgtc tgactgccag ttcgccagcg aattgcggta
    37021 ttcccattgg ctaatgaatg ctggcagaga ttctggctta acgcggaaga agttaacatc
    37081 gacattatta acgttgagcg ccattaccgg cagcccttca acgactttgc caggcagcag
    37141 cgaaccacgg ctggcaaaac cgacgctggg ttggatgtcg cgggtagtta tagttttttc
    37201 gtaatcttta ctgaaggttg cgttgttgag tgctttgacc tccttgccaa tagtaacgat
    37261 caaatcacgt ttcggttcga ggtggcgtaa acgtagctct ttaagattat ctgacagctc
    37321 ccaggcacca tccactttgc cgcttttttt atcgacgaca tgaataacgc gtgagaaatc
    37381 ctgatccggg tcgagaggga tggagaacgt cagcaccagc gtggctgcac catcaagttg
    37441 gacttcagag agatccagca atgtcagcac cttaccggca ctctgttggg ccagtttttg
    37501 tctctccggc acggagagtt ttgctgaact cgcgttttct gaagaggccg ctttagtaac
    37561 ttcagaaggc gcatcttttt tcaccgctgt tggcgcgtta tcgttgttgt cgcaccctgc
    37621 cagcgccagc attagcatgc aggcggctac gcgtaacttt ttcatttttc atccctgcca
    37681 caatggcccg ttagcaacgt cgaataatta ttatgcgtga gaatttacgt tatgtaattc
    37741 agtatcacgc ccatacaatc cgaattttag tgagaattgt ggctcagaca gccaaatcag
    37801 cctctttcgc ttgtcgcttt tcactaaaca tccgacaatt tagcctaacc ccggcaaaaa
    37861 tggagatgcc tatgtccacg acatggtttg taggagccga ctggctcgcc gaacatattg
    37921 atgacccgga aattcagatt atcgatgccc gcatggcgtc gcctggacag gaagatcgta
    37981 acgttgctca ggagtatctg aatggacata ttcccggcgc agtgtttttt gatatcgaag
    38041 cgctttctga tcacacttcc ccgcttccgc acatgctgcc gcgcccggaa acgttcgccg
    38101 tggcgatgcg tgaattaggc gttaaccagg ataagcacct gattgtctat gacgaaggta
    38161 atcttttctc agccccacga gcatggtgga tgctgcgcac ctttggtgta gagaaagtgt
    38221 cgattctggg gggtggactt gcaggctggc agcgcgatga tctgctgtta gaagaaggtg
    38281 cagtagagct gccggaagga gagtttaacg ccgcgtttaa tcctgaagcc gtggtgaaag
    38341 taaccgatgt attattggca agccatgaaa atacggcgca aattattgat gcccgcccgg
    38401 ctgcacgttt taacgcagaa gttgatgaac ctcgcccagg tttacgtcgc ggacatattc
    38461 ccggagcact gaatgttccg tggacggaac tggtgcgcga aggcgaacta aaaacgaccg
    38521 atgaactgga tgcgatattt tttggtcgcg gcgtcagcta cgacaaacca attatcgtca
    38581 gctgcggctc tggtgtaacg gcagccgtgg ttttgttagc actcgcgacg ctggatgtgc
    38641 caaacgtgaa actgtacgac ggcgcatgga gtgaatgggg cgcgcgggca gatttaccgg
    38701 ttgagccagt gaaataagta ttttacaggc aataaaaaac cgccgaattt ggcggttttt
    38761 tattgctggt ctggttcgcg gcctttccag caggttgtat taccgtagta atgcaagcgc
    38821 gtctcagcgg agacaatact cgccagtaac tctctttttg tcaagcaaaa gagagttatt
    38881 attgttctgt tagtgtatta tccactgcgg ccctttccgc cgtctcgcaa gcgggcgctg
    38941 gcttaaggaa aggatgttcc gcgaccgtta gagcaagcgc gtctcagcgc ttgctatcgc
    39001 ggcaatatcg ccagtggtgc tgtcgtgatg cggtcttcgc atggaccgca caatgaagat
    39061 acggtgcttc tgtatcgtac ttattgtttc tggtgcgctg ttttcccaag taaataataa
    39121 ccggagtctc tccggcgaca atttactggt ggttaacaac cttcagagca gcaagtaagc
    39181 ccgaatgccg ccctttaggg cggcatattt tagattatcc ggttttgttt aaagtcacgc
    39241 aaaaaaccac cccagcgacg ttcatagaat ggcacaatat gttcggtaat aaagtggcta
    39301 attccttttt cccctttttt cacctgacaa atatcgattg gttcatcgcc aggtaatgta
    39361 tcggtcgcta cacttcccgt cgcctgaata atttcttcga tatcaccatc ggcttcaatg
    39421 ccaataagta aattaggctg tgcctcttcg ttctctttaa ttgaacaaat aaaagcacgc
    39481 ttcaccggct taatggtttt aaataaggtg gtgagtgaat caatcatttg tgctggcggc
    39541 tctgcgactt ccgataatat cagcgattca ccgccttcca ggatttcctg gctgctcagc
    39601 ggatttccct cttcaccaat caacaaactg atttcacgcg gcataaattc tttaccggtc
    39661 ggcagtttgg cattaaggaa gagcgtttcg ccaagggtca tctcaaacaa tgtgcgaacg
    39721 ggcattacga caaatgcctg ttcgtcttca accgcctgtt gaagcgcttc taacgaggtg
    39781 aaaaaaggaa tgacgctggt gccgtcttct ttttcccagt gctgtaaatc aagcgcacta
    39841 tcttcaacca cagcctcgcc ctgcgccgcc gtaccaggca cccagacggt ggattccagt
    39901 agagtacgga aaaaggccgg gcggtgcgcc ggttcggttg ctgctttttc cagcaggtct
    39961 tcaagttcgt tttttgtttc ggacataaga accacaattc attcaacgtt cggggcgcaa
    40021 atgcctgatg cgctacgctt atcaggccta caagggattc gcaatttgtt gaatttgcag
    40081 aatttgtagg ccggataagg cgtttacgcc gcatccggca tattagttta cgccgttaac
    40141 agattagcta tcgtgcgcac accaagtccc gtagcgcccg cagaccactg ttcaaccggc
    40201 gctttacggt aagtcgccga gcagtcgata tgcagccagc cttgctgata gttctcaaca
    40261 aagtgcgaca ggaagcccgc cgccgtgctc gcgcctgccg gatacgccgc gcttccggta
    40321 ttgttcagtt cggcaaagtt agacggcagc tggctgcggt ggaattccgc cagcggcaga
    40381 cgccagaatg gttcgttctc ttgtgaggca ctcgccagca aacgaccggc aagcgcatcg
    40441 tcaaaactga acagcgcgtg ataatcatta cccagcgcag ttttcgccgc cccggtgagg
    40501 gtcgccgcat caatgatcat ttccggtttc tgcgcactgg catcaatcag accatcggca
    40561 agcaccagac gcccttcggc atcagtgttc atcacttcaa cttttttacc gttgcgatag
    40621 gtgatgatat cgcccagctt gaacgcattg ccgctaatca ggttatccgc acagcagagg
    40681 aacagcttca cgcgcttgtt cagtccgcgc gtaatggcaa atgccagcgc cccggtaacc
    40741 gttgccgcgc cgcccatgtc cgacttcatc gagtccataa acgcagtctg tttgatgctg
    40801 tagccgccgg agtcaaaagt gataccttta cctaccaggc acgcgtacac tggcgcttct
    40861 ttatcgccag ttgggttgta atccagcgcc agcaataccg gagaacgttc tgaaccgcgt
    40921 ccgacggtgt gcagccccat ataaccttgc tcacgcagat cttcgccttt ggtgatccga
    40981 taagtcacac gatcgcccgc gacgttgctg atcagatcaa cagcacgctg tgccagttgc
    41041 gatggtccca attcttctgc cggtgcgttg atggtgtcac gcacccagtc gatgatcatc
    41101 aggcggttat ccagttcctg gcgctgggca tcgtccagat ccggccactc cactttacgc
    41161 gtgcctttcg gggctttgta accttgccag aatgcccagc agcgatccgc atcccagcct
    41221 tcaccgctta actgaacatg cttgatgccc agaccgtcaa tcttgcgcgc cgcacgctgg
    41281 atcagcccca gatcgtctgc cccgttcaaa tgcagggtaa tgccgtcatt attaatgctg
    41341 taagttgctt tttctcccca gcgcgcgtcg gcaggttggg tagagagggt aatcttcatc
    41401 gcttctgtca ttttagttat ccttctttgt aaaagggccg ccagttggca gccccaggat
    41461 ttttccgcta aatgattcgc gttgcagaaa ggcggcaagt gagtgaagcc ccaggagcat
    41521 agataactat gtgactgggg tgaacgagcg cagccaacgc atctgcggcg tgaagcatga
    41581 cgcggaaatt attcggcctc gtccagccag actaacaaaa tcgcttcgag gattttctcg
    41641 ttggatgcct gcgggtcgtc gtcgaaatct tccagatcgc aaatccactg atgcatatcg
    41701 gtgaatcgaa ccgttttcgg atcaagatca ggatacgcat cgtacagtgc ttcgccaatt
    41761 tcgcggctat cggtccactt aagtcccata ctaacctctg ttaatgctca cgcgcatggt
    41821 tgatagtgta acgcgggatt tcgactacta aatcttcgtc ggtaacacgt gcctggcagc
    41881 ttaaacggct ttcgggctcc agtccccagg ctttgtccag catgtcgtct tcctgctctg
    41941 agctttctgg cagcgagtca aaaccttcac gaacgatgca gtggcaggtg gtgcaagcac
    42001 aggatttttc acaggcgtgt tcaatctcga taccgttacg cagagctgcg tcgagaatgg
    42061 tttcaccgct attagcttcc agaacagcgc catcaggaca gagatcctga tgaggcaaaa
    42121 taacaatctt tggcatatta aacctcgtcc acggaatggc ctttcagcgc acgacgaacc
    42181 gactggtcca tgcggcgagc ggcgaaatcc tgggtttgtt tgtctacgtt tttaatcgct
    42241 tgttcgatgg cgtcaacgtc atcgccctgc gccacttcac tcaggtgagc ggcagcatcg
    42301 tcaatgacct gacgttctgc ggcgcttaac agcgcggcat cagcagccag cgcgccgtgc
    42361 agactttcca gcacacgcgc cgcttctact ttttgttctg ccagcattcg ggcttttacg
    42421 tcctgctcgg catagctcat tgagtctttg atcatcgaag cgatttcgct atcggtcaga
    42481 ccgtaagacg gtttgacctg aatagacgcc tcaacgccgg tggatttctc catcgccgtc
    42541 acgctcaaaa gaccgtcggc atcgacctgg aacgtcacgc gaatatgcgc accaccagcc
    42601 ggtagcgccg gaataccacg cagcgcaaaa cgcgccagtg agcggcagtc ctgcaccagt
    42661 tcgcgctcac cctgcattac atggatagac atcgccgtct gaccatcttt aaaggtggtg
    42721 aaatcctgag cgcgggccac cggaatagtg gtattacgcg gaatcacttt ctccaccagg
    42781 ccgcccatcg tttcgaggcc cagcgacagt gggatcacat caagcagcag catttcgctg
    42841 tctggcttgt tacccaccag aatatccgcc tgaatcgccg cgccaatagc gacgacttta
    42901 tccgggtcga tggaagtcag cggtggacga ccgaaaaatt cgcctacccg ttcacgcacc
    42961 agcggcacgc gagtagaacc gcccaccatc accacttcca gcacttcgtc agcttctaca
    43021 cccgcgtctt taagcgcgcg acgacaagcc agtaaggttc gttttaccag tggcgcgatc
    43081 agttcattga attgttcacg gctgatttcg ccctgccagc ccgcaacgtt aacggtcacg
    43141 gagtccgcat cgctcagcgc gattttggct gcaatggcgg catccagcag ttcacgctga
    43201 acgcggttat cgctacgatc aggaatgccc gcctgctcgc gaatgtaatc cgccagcaga
    43261 tggtcgaaat catcgccgcc gagcgcggaa tcaccgccgg ttgccagcac ttcaaacacg
    43321 ccgcgactta agcgcagaat ggaaatatca aacgtcccgc caccgaggtc ataaacagcg
    43381 atcacgcctt cctgaccgga atccagcccg taggcgatag ccgcagcggt cggttcgtta
    43441 agtaagcgca gaacatgcaa ccccgccaga cgcgccgcgt ctttggtgcc ctgacgctgg
    43501 gcatcgtcaa agtacgccgg aacggtgata actacaccat ccagctcgcc tgccagggct
    43561 tcagttgccc gcgccgccag tgctttgagg atgtccgcag aaacgcgcac cgggttcagc
    43621 agccccgccg ccgtttcaat catcggcagg ccgttttcgc tggcctggaa ttgataaggc
    43681 agatgcggat agcgttgctg gatatcagcc agcgagcgcc ccatcaggcg tttaacagaa
    43741 ctaatggtgt tggcggtgtc aagcgctgcg ttagtacgcg cgtcataacc caccgaatgc
    43801 ccttgctgtt gatagtgaac aacagatggc agcaggtgac ggccttcatg gtcggctaac
    43861 gtttcggcct gaccgctgcg cactgtcgcc accagcgagt tggttgtgcc caggtcaata
    43921 ccagccgcca gacgacgctg atgcggcgca gcactcaaac caggttcact aatttgtaat
    43981 aaggccatgt ttagcttcca gaaattaaaa atcgagcagt ttttcttcga gttgttcggc
    44041 actgctccgc agtttatcga gaaaacgcag cttacgcacg gtatccgccg ccgcgtccca
    44101 cgcctcgttg tctaactgtt caaccatcaa ctgatggcgg gtatcaaaca tctttttcac
    44161 acgtttgata aagctttcca gccgcgcttc atctttcgcc tgttcgatct cgtccagctc
    44221 ttcgcgcagc tccaactgtt ccatcaggaa cgcggtgtcg cgcacagtat gctgctcgct
    44281 ggcgagatca aaaccgtgta aagaaagcaa atattccgcg cgcattaacg gatgacgcag
    44341 cgtttgccag gcctggttaa tggttgcaga ttgctgtacg gcggcgagtt gttccgcctg
    44401 gcttccgctg gcgaatttat caggatgata ctgacgttgt agatcctgaa aacgcaggct
    44461 cagcgcctgg gtatcgagtt gataacgggc aggcaagcca aagaaggtga agtaatccat
    44521 aacattctca gggctgcgat ccgccgcgat aacggcgaat caggtaaaac aaaccccacg
    44581 cgcaggcgac cacggtgggg ttatcggtat gcgcatcaaa cgtggaagct ttcgccgcaa
    44641 ccacactcat ctttgacgtt cgggttggtg aatttaaacc cttcgttcag gccttctttt
    44701 acgaagtcca gctgcgtacc gtccagaaat tgcaggcttt tgccatcgac cacgactttc
    44761 acgcctttgt cttcaaacac gatgtcttcc ggcgtcggtt cgtcaacaaa ttccagtaca
    44821 taagccatac ctgaacatcc ggaggttctc acgcccagac gcaggccaaa ccctttgccg
    44881 cggttagcca gaaaggtatt tactcgcgct gctgcactgt cgctcagtgt aatcgacata
    44941 accaaacctc aactcttatt ttgcttcacg tttgctttta tagtccgcaa tggcggcttt
    45001 gatcgcgtct tctgccagaa tagaacagtg aattttcacc ggcggcagtt caagttcttc
    45061 agcaatatcg gtatttttga tcgcctgcgc ttcgtcgaga gacttccctt tcacccattc
    45121 ggtgaccagg gagctggaag cgatagcaga accgcagccg taagttttaa aacgcgcgtc
    45181 ttcaatgata ccttcatcgt tgactttaat ctgcaacttc atcacgtcgc cacaggccgg
    45241 tgcccccacc atgccgctgc cgacgttctc gtcgttgttg tcaaaggaac ccacgttacg
    45301 cggattctcg taatggtcga taactttttc gctgtaagcc attataaatt ctcctgattc
    45361 cgataccgat taatgatgag cccattcgat gctgttcaga tccacgccct gcttgtacat
    45421 ttcccacagc ggagaaaggt cacgcagacg accgatggat ttacgaacta actcgatggt
    45481 gtagtcgatc tcttcttcag tagtaaaacg acctaaagag aaacggatag agctatgtgc
    45541 cagctcgtcg ttcagcccca gcgcgcgcag cacgtaggac ggttcgaggc ttgctgacgt
    45601 acaggcggaa cctgaagaaa ctgcgaggtc tttcagcgcc ataatcagcg actcaccttc
    45661 aacgtagttg aagctgacgt tgagaatgtt cggcgcaccg tgttccaggt caccgttcag
    45721 gtaaacttct tcgatatctt tgatgccgtt ccacagacgg ttacgcaggc cgcgcagacg
    45781 ttccatctcg gtcgccatct cttcttttgc gatgcgatag gcctcgccca ttccgacgat
    45841 ctggtgaaca ggcagagtgc cggaacgcat accgcgctcg tgaccgccgc cgtgcatttg
    45901 cgcttcgatg cgtacgcgcg gtttacgacg tacatacagc gcaccgatac ctttcgggcc
    45961 atagattttg tgaccggaga aagacatcag gtcaactttc aactggctca ggtcgatagg
    46021 cagtttgccc acgctctggg ttgcatcaac gtgatagata atgccacgag cacggcacat
    46081 ttcgccgata gccgcgatat cctgcaccac gccgatttcg ttatttacgt gcatgatgga
    46141 aacaaggatg gtgtcatcac gcatcgctgc ttcaagttct ttcagatcga taatgccgtt
    46201 acgctgcggt gccaggtagg tgacttcaaa accttcgcgc tccagctgac ggcaggtatc
    46261 cagtaccgct ttgtgttcgg ttttgctggt gatgatgtgc ttgccttttt tctgataaaa
    46321 gttggctgca cctttgatcg ccaggttgtc agattcggtt gcaccagagg taaagacgat
    46381 ttcacgcgga tcagcgccga ccagatcggc aatctgatta cgggcgatat ctaccgcttc
    46441 ttcagcctgc cagccgaaac ggtgagaacg ggaggccggg ttaccaaagg ttccgtccat
    46501 cgtcataaac tgcatcattt tctcggcaac acgcgggtcc accggcgtgg ttgcggagta
    46561 gtcgagataa atcggtaatt tcattgctct ataaactccg tacatcactc aatgcaagga
    46621 accaggctac cggctggatg tacgaccgtg tttacggagc gcgatacgcc ccggcctgat
    46681 tctaatatct tgttatcttt aacctaactg tttcgggttt cagcaagttg acaaacttac
    46741 gaatctccgg gagcttacat aagtaagtga ccggagtgag tatgtgcagt caacgcagat
    46801 gcaacacgaa acaggacggt taaattaagc gcgtaactta acgtcgatcg cgtcttgtat
    46861 gcgggtgcgt ggcgcgtcgt gagtatgctg acgaccagac acatccagca cttcctggtt
    46921 attaaccagt tcgcctaaag taatgttgtt gagaaaaccg gtgagacggt cgctcaaatc
    46981 acgccacagc gcgtgggtca ggcatttatc gccgccctgg caaccgcctt taccctgaca
    47041 acgggtagca tctacagatt cgtcaacggc gctaatcact tcgccaacgg cgatgctgct
    47101 ggcatctttg cctaacagat aaccaccgcc tggtccacgt acgctggaaa ccagaccatt
    47161 tttacgcaga cgggaaaaca gttgttccag ataagaaagg gaaattccct gacgttcgga
    47221 aatatcagcc aacggtaccg ggcccgcttc agagttgagc gcaacgtcaa gcattgcggt
    47281 cacggcatag cgccctttag atgtcagtct catgtcttac ttcacctcaa actcgcccct
    47341 gcccggggtt ttttattgta aaagtggggg tattgcatag cagggtcaag tctgacattc
    47401 ccgagtaaat tggtcaacta tttacttgac tgatttagtc gggtatttaa ccttcagtgc
    47461 cattttttta tcgtggcgtt tgtacctgtt gtccttccag accatgggcg ggttgagggc
    47521 aattattcgc attagcgctg gcctcttccc ctcaccctaa ccctctcccc gaaggggcga
    47581 ggggaccgtc cgtgcattta tctggcttag ccctcaggcc ttattacctt tattctgctg
    47641 ctctatagaa gccagaatcc cgcgcaggat attcaattcc tggctttccg ggcgcgcacg
    47701 ggtaaacaga cggcgcaatt tattcatcac ctgccccgga tggttttcac ggataaaacc
    47761 ggttgccagc agcgtttgtt ccagatgacc ataaaaacgc tccagatcat cgaccagcgg
    47821 atacggcgtc tcttcatgct cgacctgctc gccattttcc tgagttgcca gccaggccat
    47881 acgcacttca taggcaataa cctgaaccgc cattgccaag ttcagcgaac tgtattccgg
    47941 gtttgccgca atcgcgacat gataatggca tttctgcaac tcttcattgg tcaagccgac
    48001 gcgctcacga ccaaacacca gcgccaccgg ggtatttgcc gcttcagcga cgcttttcag
    48061 accgcattcg cgcgggtcga gcatcggcca cggcagcgtg cgggagcgtg cgctggtgcc
    48121 caccaccagg ctacaaccgg ctaacgcttc gtcgagcgta tcgacgatgt gagcattacc
    48181 aatcacatcg ctggcccctg ctgccagggc aatcgcctgg gagtcgggtt tcaccagtgg
    48241 attaaccagc cacagattgg ttaatcccat tgttttcatg gcacgggcaa cagaacccat
    48301 attgccggtg tgtgacgtct ccaccagcac aattcgaata ttttgcagca ttgtctttct
    48361 tcatctaaag attattcacg catcttatca taaaacgaag acagatgccg atctcgctgc
    48421 tatactctgc gccgttttcc cgttctttaa catccagtga gagagaccga tgcatccgat
    48481 gctgaacatc gccgtgcgcg cagcgcgcaa ggcgggtaat ttaattgcca aaaactatga
    48541 aaccccggac gctgtagaag cgagccagaa aggcagtaac gatttcgtga ccaacgtaga
    48601 taaagctgcc gaagcggtga ttatcgacac gattcgtaaa tcttactcac agcacaccat
    48661 catcaccgaa gaaagcggtg aacttgaagg tactgatcag gatgttcaat gggttatcga
    48721 tccactggat ggcactacca actttatcaa acgtctgccg cacttcgcgg tatctatcgc
    48781 cgttcgtatc aaaggccgca ccgaagttgc tgtggtatac gatcctatgc gtaacgaact
    48841 gttcaccgcc actcgcggtc agggcgcaca gctgaacggc taccgtctgc gcggtagcac
    48901 cgctcgcgat ctcgacggta ccattctggc gaccggcttc ccgttcaaag caaaacagta
    48961 cgccactacc tacatcaaca tcgtcggcaa actgttcaac gaatgtgcag acttccgtcg
    49021 taccggttct gcggcgctgg atctggctta cgtcgctgcg ggtcgtgttg acggtttctt
    49081 tgaaatcggt ctgcgtccgt gggatttcgc ggcaggcgag ctgctggttc gtgaagcagg
    49141 cggcatcgtc agcgacttca ccggtggtca taactacatg ctgaccggta acatcgttgc
    49201 tggtaacccg cgcgttgtga aagccatgct ggcgaacatg cgtgacgagt taagcgacgc
    49261 tctgaagcgt taatgactca ggcgggtgat gtcactcacc cgccctcgcc tttcaggcgc
    49321 tattctgaaa tacttcctca ccgctttact ttctttcgtc actctcccac catctttccc
    49381 cgattaatgg atgataaaga agtcacaagg aaaggttatg gcgctgccag tgaacaaacg
    49441 cgttctcaaa attctgttta ttctctttgt tgttgccttc tgcgtttatt tagtgccgcg
    49501 cgttgccatc aacttcttct attatcctga cgataaaatt tacggtcccg acccctggtc
    49561 ggcggaatcc gtcgaattta cggctaagga cggtactcgt ctgcaaggct ggtttattcc
    49621 ttcttcgacg ggccctgctg acaatgccat cgcaaccatc attcatgctc acggcaatgc
    49681 cggaaatatg tccgcccact ggccgctggt cagttggtta cccgagcgta atttcaacgt
    49741 ttttatgttt gattatcgcg ggtttggtaa atcaaaaggc acgccgtccc aggccggatt
    49801 gctggacgat acgcaaagtg ccatcaatgt ggtgcgccat cgcagtgatg taaacccaca
    49861 acggctggtg ctgttcgggc agagtattgg cggggcgaat attcttgctg ttattggtca
    49921 ggatgatcgt gaaggcatac gtgcggtgat cctcgactcc acatttgcct cttatgcaac
    49981 catcgccaat cagatgatcc ccggcagtgg ctacttactt gatgagagtt acagcggcga
    50041 aaattatatc gccagcgtca gcccgatccc gcttttactc attcacggta aagctgatca
    50101 cgttatccca tggcagcaca gcgaaaagtt gtatagcctg gcaaaagaga caaaacggtt
    50161 gatcctaatc ccggatggcg aacacattga tgctttttcc gatcgtcacg gcgatgttta
    50221 tcgcgaacag atggtggact ttatccttag cgcgttgaat ccgcagaact aacccatgat
    50281 cgctagcacg ataatcattc acaaaaccac cttaagacat gctaatccac tggtcagaac
    50341 agtttaagat gagaaaaatt ctgtgacgct tgccaacatt tctgatgatt agcattccct
    50401 tcgccatttc cctgagcaaa ctttagctat tcttatcaat tatgcttatg ggagatctac
    50461 agatgatgcc tacgcttgct ccaccatctg tcctttcggc tccccagcgc cgctgccaga
    50521 tcttgctgac gctctttcag ccggggttga ccgccaccat ggcaaccttc agcgagctta
    50581 atggtgtgga tgatgatatt gccagtcttg atatcagcga aacaggacgg gagatcctgc
    50641 gctatcatca actcacactg acgactggtt atgacggtag ctaccgggtt gaaggtacag
    50701 tgcttaacca acgtttgtgt ttatttcact ggctacgacg tggtttccgt ctgtgtccgt
    50761 catttattac cagccaattc acccccgccc tgaagagtga actgaagcgg cgcggaattg
    50821 cgcgtaactt ttacgacgat accaatctac aagcgttagt gaatctctgc tcccgacggc
    50881 tgcaaaaacg ctttgaaacg cgcgatattc atttcctgtg tctgtatctg caatattgtt
    50941 tgctgcaaca ccacgctgga attacgcctc agtttaatcc gctccaacgt cgctgggcgg
    51001 aatcctgcct tgaatttcag gtagcgcagg aaattggacg ccactggcag cgtcgggcgc
    51061 tccagcctgt accgcctgat gagccactgt ttatggcatt acttttttcc atgttgcggg
    51121 ttcccgatcc attgcgtgat gcgcatcagc gagacagaca attgcgtcag tctatcaaac
    51181 gtctggtaaa ccattttcgt gagctgggaa atgttcgttt ttatgatgaa caggggttat
    51241 gcgatcagct ttatacccac ctcgcccagg cgttaaatcg cagtttgttt gccatcggta
    51301 ttgataatac cctgccggaa gagttcgcca gactgtaccc acgcctggtg cgcaccaccc
    51361 gcgcggcgct ggccggattt gaaagtgaat acggcgtcca tctttctgat gaggaaagcg
    51421 gtctggtcgc ggtgattttc ggtgcctggc taatgcagga aaacgatctg catgaaaaac
    51481 agattattct actgaccggg aatgatagcg agcgagaagc gcagattgag cagcagttac
    51541 gcgaactaac gttactgccg ctcaacatta agcatatgtc ggtaaaggca tttttgcaga
    51601 caggcgctcc gcgtggcgcg gcactgatta ttgcgcctta taccatgcca ttaccgctct
    51661 tttcaccacc gctgatctat acggacctga cgctgacaac acatcaacag gagcagatcc
    51721 gcaaaatgct cgaatcagca tgagggaaca actttcgggc gcaaaaacat tgccggaagc
    51781 gccaccagcg ccattaccca gaacacgccg tggcccagat attgatacag gaaaccggcg
    51841 aaaacggtca tgatagcgat actgccgccc atcgcgacgg cagagtaaac cgcctgtaaa
    51901 cggatgactt cgctaccctg gcgaacagca atatagcgca tggcggccag gtggcagacc
    51961 gtgaaggtgc cgcaatgcaa aatttgcacc actatcaacc acggcaacgc cgtagttgct
    52021 cccataatgc cccagcgcac tacgccgcaa atcgccgaga tcaacagcat atcgcgagca
    52081 ctacaacggc ggaaaagttt attactcagc gcaaagataa tgacttccgc caccacgccc
    52141 agcgaccaca aataccccac cgccgaggcc gagtagccag ctgcctgcca gtaaatggcg
    52201 ctaaaaccgt aataggccgc atgtgccccc tgcaataaac aaacgcaggc cagaaagcgc
    52261 cagttctggc gaaccagcgc caaccacgca gaccaaccgg tgctctcctg ctggcgactt
    52321 gccccttgtg gctgaatcgt cggacggatg agaaagccga gcagcatgga tgccacgccc
    52381 aacgtcaaca gcgcgaggat cacccgataa tcaaacatag tgaccagttt gcccgtcagc
    52441 gccgagccaa tgacaaacgc caccgagccc cacagtcgca ctttgccgta atcaagcggg
    52501 aactgctttt gccacgtatt cgccagtgca tcggtcaacg gtaccagcgg tgagaaaaag
    52561 aggttaaagc caatcatcac cagcatcagc cacgctacgt gcgcccccgc ccagaaggcg
    52621 acagcaaaga gaagtgtcag cagtgccagc acgcgcaagg cggaaatcag gcgggaagga
    52681 tcgctgacgc ggggcgcgat gagcaaactc ccgagaaaac gggcaaccag acctgccccc
    52741 aataacaggc cgatggtttc tggcgttaaa ccaatccctt taagccagac gctccagaaa
    52801 ggtagaaaaa tgccgtaact aaaaaagtat gtgaaatagc cgagcgccaa ccagcgcgtg
    52861 gattgcaaaa ccatgagtcc ctcccgtcag gaggcgttag tctggggtta atcgaatgtt
    52921 ggtgcaagtt gaaaaataag aatattcgag cctgcttccc gatgtctgta acgccagttc
    52981 acaagcacta aaaaactgcg atccgcgacg catgttgcaa aaataatgcg taattatgcc
    53041 gttacgcttg ccaaacgttc ctgcacaatg gcgatgaaat cgcgtaatgc cggcttcatc
    53101 tcccctttct tccacgccat cagtaaagca atggaaggca catttccggc aatgggacga
    53161 aaaacaacct gcccggtatt aaaattattc atataaccgg gtatcaaagt gacgcccagc
    53221 cccatgccca ccagattcat ggtcaccaga atattcgttg ccacctggac gatatttggc
    53281 tggctgtttt cttgcgcaaa ccacgcttta acgatcggcg caagcgaacc ggaatacgcc
    53341 ggatcggtac tgacgaaatt cacgccatcc agttacgcgg cggtgatctc tttttcatgt
    53401 gctaaagggt gatcaaccgg taacaccacc actaatggtt cgtcaaaaag ctccagataa
    53461 tcaatctccg ggctataaac aggatggcgc attaagccga cgtcgagttc accacggcga
    53521 attttttcct cctgttgcgt ggtgattaaa ctcaccagct caatcaaggt gtctggctgt
    53581 ctgagacgaa acattggtaa tacttttggc aataaattca cttccgccga tggcacaaag
    53641 ccaatggtta attgtctgtc ttcctgaaca attttccgcg cccgtaattt ggcattttcc
    53701 gcttgttcga ggattgccag cgcgtcctgg agaaaacatt ctcccgccgc cgtcaacgcg
    53761 actttgcgct tatccctcac cagtaacgga acaccgacac agttttcaag atcgcggatc
    53821 tggctgctta acgaaggctg tgaggtatgc agtttttccg ccgcacgggt aaagttcagt
    53881 gcctgcgcca ctgcgacgaa atagcgtaaa tgccgtagtt ccatcacctt ccccttgtta
    53941 tcgaaaaaac gtctcagctg gtagaaatta actatttcac atattagcaa ccaaccaaca
    54001 acatccttat gacacaaaaa tagaaggtca atacatctta tctttcagga ttaaaaaata
    54061 tgaccacacc ctcagattta aacatttacc aactgattga tacccaaaat ggtcgggtca
    54121 ctccgcgtat ttataccgac ccagatattt accaactgga gcttgaacgt attttcggcc
    54181 gttgctggtt atttctcgcc cacgaaagcc agatcccaaa acctggtgat ttctttaaca
    54241 cctacatggg ggaagatgcg gttgtcgtag tgcgtcagaa agacggcagc attaaggcgt
    54301 ttctcaacca atgccgccac cgggccatgc gtgtgagtta tgcagattgc ggcaacactc
    54361 gcgcctttac ctgtccgtat cacggctggt cttatggcat taacggcgag ttgatcgatg
    54421 taccgctgga acctcgcgcc tacccacaag ggttgtgtaa atcccactgg ggactaaacg
    54481 aagttccttg tgtggagagt tataaagggc tgatttttgg caactgggat accagcgcac
    54541 cgggcctgcg tgattacctg ggtgacattg cctggtatct ggatggcatg ctggatcgtc
    54601 gcgaaggcgg caccgaaatt gtcggcggcg tacaaaagtg ggtgatcaac tgtaactgga
    54661 aattcccggc agagcagttc gccagtgacc agtatcatgc tctgttcagc catgcttctg
    54721 ccgttcaggt attaggggcg aaagatgatg gcagcgataa gcgcctcggt gatggacaaa
    54781 ccgcccgccc ggtgtgggaa accgccaaag atgcgctgca atttggtcag gacggtcacg
    54841 gtagcggttt cttctttact gaaaaaccgg atgctaatgt ctgggtcgat ggcgcagttt
    54901 caagctatta ccgcgaaacc tatgccgaag cagaacaacg tttaggtgaa gttcgcgccc
    54961 tgcgcctggc gggtcataac aatattttcc ccacgctttc atggctcaac ggcactgcca
    55021 cgctccgcgt ctggcatccg cgcggccctg atcaagttga agtgtgggcg ttctgtatta
    55081 ctgacaaagc cgcctccgat gaagttaaag ccgcttttga aaacagcgcc actcgtgctt
    55141 ttggtcctgc tggttttctc gagcaggatg actcggagaa ctggtgtgaa atccagaaat
    55201 tgcttaaagg ccaccgcgcc cgcaacagca aactgtgtct ggaaatgggg cttggtcagg
    55261 aaaagcgccg cgacgacggc attcctggca ttactaacta tatcttttca gaaactgccg
    55321 ctcgtggaat gtaccaacgc tgggccgatc ttctgagtag cgaaagctgg caggaagtgc
    55381 tcgataaaac cgccgcttac cagcaggagg tgatgaaatg agtgcgcaag tttcactaga
    55441 gttacatcac cgcattagcc agtttctctt tcacgaagcc agcttactgg acgactggaa
    55501 atttcgtgac tggctggagc agctcgacaa agagattcgt tacaccatgc gcaccacagt
    55561 taacgcgcaa acacgcgacc gccgcaaagg cgtccagcca ccgacaacct ggatttttaa
    55621 tgacaccaaa gaccagctgg agcggcgaat cgcccgtctg gaaacgggca tggcctgggc
    55681 agaagagccg ccgtcacgca cccgtcactt aatcagcaac tgccagataa gcgaaaccga
    55741 catcccaaac gtatttgctg tgcgggtaaa ttatctgctt tatcgggcac aaaaagagcg
    55801 cgatgaaaca ttctatgttg gaacgcgttt cgacaaagtt cgccgtctgg aagatgacaa
    55861 ctggcgcttg ctggaacggg atatcgtcct ggatcaagcg gtaatcactt cccataacct
    55921 gagtgtactg ttctgatgaa tcgaatttat gcgtgtcccg ttgcggatgt gccggagggt
    55981 gaggctctcc ggatcgatac ctcgcccgtc atcgccctgt tcaacgttgg cggcgagttt
    56041 tatgccatta acgatcgttg cagccatggt aatgcgtcga tgtcagaagg gtatctggaa
    56101 gatgacgcca cggtggagtg cccgctacac gccgccagtt tttgcctgaa aacggggaaa
    56161 gcgttatgcc tgcccgccac cgatccgctc accacttatc cagtacacgt tgaaggtggt
    56221 gacattttca tcgacttacc ggaggcgcag ccatgagcga tctgcataac gagtccattt
    56281 ttattaccgg cggcggatcg ggattagggc tggcgctggt cgagcgattt atcgaagaag
    56341 gcgcgcaagt tgccacgctg gaactgtcgg cggcaaaagt cgccagtctg cgtcagcgat
    56401 ttggcgaaca tattctggcg gtggaaggta acgtgacctg ttatgccgat tatcaacgcg
    56461 cggtcgatca gatcctgact cgttccggca agctggattg ttttatcggc aatgcaggca
    56521 tctgggatca caatgcctca ctggttaata ctcccgcaga gacgctcgaa accggcttcc
    56581 acgagctgtt taacgtcaac gtactcggtt acctgctggg cgcaaaagcc tgcgctccgg
    56641 cgttaatcgc cagtgaaggc agcatgattt tcacactgtc aaatgccgcc aggtatcctg
    56701 gcggcggtgg cccgctgtac accgccagta aacatgccgc aaccggactt attcgccaac
    56761 tggcttatga actggcaccg aaagtgcggg tgaatggcgt cggcccgtgt ggtatggcca
    56821 gcgacctgcg cggcccacag gcgctcgggc aaagtgaaac ctcgataatg cagtctctga
    56881 cgccggagaa aattgccgcc attttaccgc tgcaattttt cccgcaaccg gcggatttta
    56941 cggggccgta tgtgatgttg gcatcgcggc gcaataatcg cgcattaagc ggtgtgatga
    57001 tcaacgctga tgcgggttta gcgattcgcg gcattcgcca cgtagcggct gggctggatc
    57061 tttaaggaag cacgatgaaa gaaaaaacga tcattattgt cggtggcggg caagcggcgg
    57121 caatggctgc ggcctcgcta cgccagcaag ggttcaccgg tgagctgcat ctgttttccg
    57181 atgagcgaca tcttccttat gaacgacctc cactctcgaa atccatgttg ctggaagatt
    57241 ccccgcagtt acagcaggtg ttacccgcta actggtggca ggaaaacaat gttcatctgc
    57301 attccggtgt aaccatcaaa acgctgggtc gcgacacacg agagttagtg ttaaccaacg
    57361 gcgaaagctg gcactgggat cagcttttta tagcaatcgg cgcggcagct cgaccgctgc
    57421 cgttgcttga tgcactggga gaacgctgct ttaccctacg ccatgccggt gatgccgcca
    57481 gactgcgaga agttctgcag cccgaacggt cagtcgtgat tgtcggtgcc ggaactattg
    57541 gtctggaact ggctgccagc gccacgcagc gcagatgtaa ggtgacagtg attgaactgg
    57601 cggcaaccgt catgggccgt aatgcaccac cgcccgtgca acgctatctt ttacagcgcc
    57661 accagcaggc tggtgtgcgc attctgctca ataatgccat tgaacatgtg gtcgatggtg
    57721 aaaaagtaga actgacgctg caaagtgggg aaacgcttca ggctgatgtg gtgatttacg
    57781 gtattggtat cagcgccaac gagcaactgg ctcgcgaggc caaccttgat actgccaatg
    57841 gcattgtcat tgatgaggct tgccgcacct gcgatcccgc gatctttgcc ggtggcgatg
    57901 tggcaatcac tcgtcttgat aatggtgcac tacaccgctg cgaaagctgg gaaaacgcca
    57961 ataaccaggc gcaaattgcc gctgccgcaa tgttagggct accgctaccg ctactgccgc
    58021 cgccgtggtt ctggagcgat cagtacagtg ataacttaca gtttattggc gatatgcgtg
    58081 gcgatgactg gctttgtcgt ggcaacccgg aaactcagaa ggcgatttgg tttaatctgc
    58141 aaaacggcgt gcttatcggt gcggtcacgc tgaatcaggg gcgtgagatt cgcccaattc
    58201 gcaaatggat ccagagcggc aaaacgtttg atgcgaaact gctgatagat gagaacatcg
    58261 cgcttaaatc actgtaacca ggataattag cgaatatctc aacgcctggg gcgtggcgag
    58321 gtgcaagagt gtgtattacg tttaaatcac attatcttgc aaagggattg gttatgaaca
    58381 cacta
//
LOCUS       DAJTZL010000005        57429 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.005, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000005 DAJTZL010000000
VERSION     DAJTZL010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 57429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57429
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.005"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            144..1487
                     /gene="argG"
                     /locus_tag="OO544_000274"
     CDS             144..1487
                     /gene="argG"
                     /locus_tag="OO544_000274"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HCR6765974.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGVPQVENLENKGQ"
     gene            complement(1495..3102)
                     /locus_tag="OO544_000275"
     CDS             complement(1495..3102)
                     /locus_tag="OO544_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011378876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HCR6765975.1"
                     /translation="MLEDFKSHWLLYLCVIVFGITNLVASSGAHMVQRLLFFVLTILV
                     VKRISSLPLRLLVAAPFVLLTAADMSISLYSWCTFGTTFNDGFAISVLQSDPDEVVKM
                     LGMYIPYLCAFAFLSLLFLAVIIKYDVSLPTKKVTGILLLIVISGSLFSACQFAYKDA
                     KNKKAFSPYILASRFATYTPFFNLNYFALAAKEHQRLLSIANTVPYFQLSVRDTGIDT
                     YVLIVGESVRVDNMSLYGYTRSTTPQVEAQRKQIKLFNQSISGAPYTALSVPLSLTAD
                     SVLSHDIHNYPDNIINMANQAGFQTFWLSSQSAFRQNGTAVTSIAMRAMETVYVRGFD
                     ELLLPHLSQALQQNTQQKKLIVLHLNGSHEPACSAYPQSSAVFQPQDDQDACYDNSIH
                     YTDSLLGQVFELLKDRRASVMYFADHGLERDPTKKNVYFHGGREASQQAYHVPMFIWY
                     SPVLGDGVDRTTENNIFSTAYNNYLINAWMGVTKPEQPKTLEEVIAHYKGDSRVVDAN
                     HDVFDYVMLRKEFTEDKQGNPTPEGQG"
     gene            complement(3580..3666)
                     /locus_tag="OO544_000276"
     tRNA            complement(3580..3666)
                     /locus_tag="OO544_000276"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3630..3632),aa:Leu,seq:gag)
     gene            complement(3681..4013)
                     /gene="secG"
                     /locus_tag="OO544_000277"
     CDS             complement(3681..4013)
                     /gene="secG"
                     /locus_tag="OO544_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HCR6765976.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(4241..5578)
                     /gene="glmM"
                     /locus_tag="OO544_000278"
     CDS             complement(4241..5578)
                     /gene="glmM"
                     /locus_tag="OO544_000278"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HCR6765977.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGSRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(5571..6419)
                     /gene="folP"
                     /locus_tag="OO544_000279"
     CDS             complement(5571..6419)
                     /gene="folP"
                     /locus_tag="OO544_000279"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HCR6765978.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(6509..8443)
                     /gene="ftsH"
                     /locus_tag="OO544_000280"
     CDS             complement(6509..8443)
                     /gene="ftsH"
                     /locus_tag="OO544_000280"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HCR6765979.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(8543..9172)
                     /gene="rlmE"
                     /locus_tag="OO544_000281"
     CDS             complement(8543..9172)
                     /gene="rlmE"
                     /locus_tag="OO544_000281"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HCR6765980.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            9298..9591
                     /gene="yhbY"
                     /locus_tag="OO544_000282"
     CDS             9298..9591
                     /gene="yhbY"
                     /locus_tag="OO544_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HCR6765981.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(9747..10223)
                     /gene="greA"
                     /locus_tag="OO544_000283"
     CDS             complement(9747..10223)
                     /gene="greA"
                     /locus_tag="OO544_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HCR6765982.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            10471..11904
                     /gene="dacB"
                     /locus_tag="OO544_000284"
     CDS             10471..11904
                     /gene="dacB"
                     /locus_tag="OO544_000284"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HCR6765983.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYITQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAQKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(12090..13262)
                     /gene="cgtA"
                     /locus_tag="OO544_000285"
     CDS             complement(12090..13262)
                     /gene="cgtA"
                     /locus_tag="OO544_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HCR6765984.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFILE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(13278..14243)
                     /locus_tag="OO544_000286"
     CDS             complement(13278..14243)
                     /locus_tag="OO544_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR6765985.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(14370..14627)
                     /gene="rpmA"
                     /locus_tag="OO544_000287"
     CDS             complement(14370..14627)
                     /gene="rpmA"
                     /locus_tag="OO544_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HCR6765986.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(14648..14959)
                     /gene="rplU"
                     /locus_tag="OO544_000288"
     CDS             complement(14648..14959)
                     /gene="rplU"
                     /locus_tag="OO544_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HCR6765987.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            15218..16189
                     /gene="ispB"
                     /locus_tag="OO544_000289"
     CDS             15218..16189
                     /gene="ispB"
                     /locus_tag="OO544_000289"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HCR6765988.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            16418..16696
                     /gene="sfsB"
                     /locus_tag="OO544_000290"
     CDS             16418..16696
                     /gene="sfsB"
                     /locus_tag="OO544_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HCR6765989.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(16744..18003)
                     /gene="murA"
                     /locus_tag="OO544_000291"
     CDS             complement(16744..18003)
                     /gene="murA"
                     /locus_tag="OO544_000291"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HCR6765990.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(18058..18312)
                     /gene="ibaG"
                     /locus_tag="OO544_000292"
     CDS             complement(18058..18312)
                     /gene="ibaG"
                     /locus_tag="OO544_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HCR6765991.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(18472..18765)
                     /gene="mlaB"
                     /locus_tag="OO544_000293"
     CDS             complement(18472..18765)
                     /gene="mlaB"
                     /locus_tag="OO544_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HCR6765992.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(18765..19400)
                     /gene="mlaC"
                     /locus_tag="OO544_000294"
     CDS             complement(18765..19400)
                     /gene="mlaC"
                     /locus_tag="OO544_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HCR6765993.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(19419..19970)
                     /gene="mlaD"
                     /locus_tag="OO544_000295"
     CDS             complement(19419..19970)
                     /gene="mlaD"
                     /locus_tag="OO544_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HCR6765994.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(19975..20757)
                     /gene="mlaE"
                     /locus_tag="OO544_000296"
     CDS             complement(19975..20757)
                     /gene="mlaE"
                     /locus_tag="OO544_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HCR6765995.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDTIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(20765..21574)
                     /gene="mlaF"
                     /locus_tag="OO544_000297"
     CDS             complement(20765..21574)
                     /gene="mlaF"
                     /locus_tag="OO544_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HCR6765996.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            21784..22761
                     /locus_tag="OO544_000298"
     CDS             21784..22761
                     /locus_tag="OO544_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HCR6765997.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIEPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            22775..23761
                     /gene="kdsD"
                     /locus_tag="OO544_000299"
     CDS             22775..23761
                     /gene="kdsD"
                     /locus_tag="OO544_000299"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HCR6765998.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            23782..24348
                     /gene="kdsC"
                     /locus_tag="OO544_000300"
     CDS             23782..24348
                     /gene="kdsC"
                     /locus_tag="OO544_000300"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HCR6765999.1"
                     /translation="MSKAGASLATCYGPVSADVIAKAENIRLLILDVDGVLSDGLIYM
                     GNNGKELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            24345..24920
                     /gene="lptC"
                     /locus_tag="OO544_000301"
     CDS             24345..24920
                     /gene="lptC"
                     /locus_tag="OO544_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HCR6766000.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGIDMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPNSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            24889..25446
                     /gene="lptA"
                     /locus_tag="OO544_000302"
     CDS             24889..25446
                     /gene="lptA"
                     /locus_tag="OO544_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HCR6766001.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            25453..26178
                     /gene="lptB"
                     /locus_tag="OO544_000303"
     CDS             25453..26178
                     /gene="lptB"
                     /locus_tag="OO544_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HCR6766002.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            26226..27659
                     /gene="rpoN"
                     /locus_tag="OO544_000304"
     CDS             26226..27659
                     /gene="rpoN"
                     /locus_tag="OO544_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HCR6766003.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDIGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHINTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            27682..27969
                     /gene="hpf"
                     /locus_tag="OO544_000305"
     CDS             27682..27969
                     /gene="hpf"
                     /locus_tag="OO544_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HCR6766004.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            28087..28578
                     /gene="ptsN"
                     /locus_tag="OO544_000306"
     CDS             28087..28578
                     /gene="ptsN"
                     /locus_tag="OO544_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HCR6766005.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVSADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            28624..29478
                     /gene="rapZ"
                     /locus_tag="OO544_000307"
     CDS             28624..29478
                     /gene="rapZ"
                     /locus_tag="OO544_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HCR6766006.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            29475..29747
                     /gene="npr"
                     /locus_tag="OO544_000308"
     CDS             29475..29747
                     /gene="npr"
                     /locus_tag="OO544_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HCR6766007.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            29961..30593
                     /gene="yrbL"
                     /locus_tag="OO544_000309"
     CDS             29961..30593
                     /gene="yrbL"
                     /locus_tag="OO544_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HCR6766008.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKLSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(30590..31318)
                     /gene="mtgA"
                     /locus_tag="OO544_000310"
     CDS             complement(30590..31318)
                     /gene="mtgA"
                     /locus_tag="OO544_000310"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HCR6766009.1"
                     /translation="MSKSRLTVFSFVRRFLLLLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFSEHWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSSPSGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(31315..31968)
                     /gene="elbB"
                     /locus_tag="OO544_000311"
     CDS             complement(31315..31968)
                     /gene="elbB"
                     /locus_tag="OO544_000311"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HCR6766010.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFATLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(32198..34534)
                     /gene="arcB"
                     /locus_tag="OO544_000312"
     CDS             complement(32198..34534)
                     /gene="arcB"
                     /locus_tag="OO544_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HCR6766011.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPVDVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(34630..35559)
                     /locus_tag="OO544_000313"
     CDS             complement(34630..35559)
                     /locus_tag="OO544_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312117.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HCR6766012.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLINRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTVHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGLAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            36234..40694
                     /gene="gltB"
                     /locus_tag="OO544_000314"
     CDS             36234..40694
                     /gene="gltB"
                     /locus_tag="OO544_000314"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HCR6766013.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGHILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHPGKALYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            40707..42125
                     /gene="gltD"
                     /locus_tag="OO544_000315"
     CDS             40707..42125
                     /gene="gltD"
                     /locus_tag="OO544_000315"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HCR6766014.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGSDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            42309..42788
                     /locus_tag="OO544_000316"
     CDS             42309..42788
                     /locus_tag="OO544_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005085993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
                     /protein_id="HCR6766015.1"
                     /translation="MESLSERTSTGYQQIHDGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIKRLSKDLSLRYKRGFSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLGIPQTPSAEFPSAKIWQTLSAKLRGCLIFPQLLYCISYFRFVYSCK"
     gene            42805..43110
                     /locus_tag="OO544_000317"
     CDS             42805..43110
                     /locus_tag="OO544_000317"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6766016.1"
                     /translation="MIYIEQLELIHKSGDVLYPVKITRKSSGKTAFHLVPFGLNKTHD
                     LLEVEDASEAIRLVIDERHSIRCSTLTATITNKKGKRIKRTGIYSIKGVNIKEYNVR"
     gene            complement(43186..43650)
                     /locus_tag="OO544_000318"
     CDS             complement(43186..43650)
                     /locus_tag="OO544_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HCR6766017.1"
                     /translation="MMMGEVRSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSTIENEQAIILKPGMFAVFMLGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(43647..44522)
                     /gene="nanK"
                     /locus_tag="OO544_000319"
     CDS             complement(43647..44522)
                     /gene="nanK"
                     /locus_tag="OO544_000319"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HCR6766018.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDVTEMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELMGADARTIFTRAGQGDEQAQQLIHRSA
                     HVLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAVFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(44519..45208)
                     /locus_tag="OO544_000320"
     CDS             complement(44519..45208)
                     /locus_tag="OO544_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HCR6766019.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPIPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSEAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(45256..46746)
                     /gene="nanT"
                     /locus_tag="OO544_000321"
     CDS             complement(45256..46746)
                     /gene="nanT"
                     /locus_tag="OO544_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HCR6766020.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(46856..47749)
                     /gene="nanA"
                     /locus_tag="OO544_000322"
     CDS             complement(46856..47749)
                     /gene="nanA"
                     /locus_tag="OO544_000322"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HCR6766021.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(47871..48662)
                     /gene="nanR"
                     /locus_tag="OO544_000323"
     CDS             complement(47871..48662)
                     /gene="nanR"
                     /locus_tag="OO544_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312126.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HCR6766022.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGEFSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVADQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
     gene            49042..50364
                     /gene="dcuC"
                     /locus_tag="OO544_000324"
     CDS             49042..50364
                     /gene="dcuC"
                     /locus_tag="OO544_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HCR6766023.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGVFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASRAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAEQKTLDNVPPLYYAILPVMPLILMLGSLFLAHVGLMKSELHLVVV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFANVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVKRTAIPMAVGFVVNMIATITLFY"
     gene            complement(50407..50904)
                     /gene="sspB"
                     /locus_tag="OO544_000325"
     CDS             complement(50407..50904)
                     /gene="sspB"
                     /locus_tag="OO544_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HCR6766024.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(50910..51548)
                     /gene="sspA"
                     /locus_tag="OO544_000326"
     CDS             complement(50910..51548)
                     /gene="sspA"
                     /locus_tag="OO544_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HCR6766025.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIELSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            complement(51943..52335)
                     /gene="rpsI"
                     /locus_tag="OO544_000327"
     CDS             complement(51943..52335)
                     /gene="rpsI"
                     /locus_tag="OO544_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HCR6766026.1"
                     /translation="MAENQYYGTGRRKNSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(52351..52779)
                     /gene="rplM"
                     /locus_tag="OO544_000328"
     CDS             complement(52351..52779)
                     /gene="rplM"
                     /locus_tag="OO544_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HCR6766027.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQVSFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(52998..54125)
                     /gene="zapE"
                     /locus_tag="OO544_000329"
     CDS             complement(52998..54125)
                     /gene="zapE"
                     /locus_tag="OO544_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HCR6766028.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSTP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLHDETRAQMDKLWLALAGGKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGDRLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            54319..54717
                     /locus_tag="OO544_000330"
     CDS             54319..54717
                     /locus_tag="OO544_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HCR6766029.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            54871..56238
                     /gene="degQ"
                     /locus_tag="OO544_000331"
     CDS             54871..56238
                     /gene="degQ"
                     /locus_tag="OO544_000331"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HCR6766030.1"
                     /translation="MKKQPQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPEQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMIAPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKSAIIALQIVRGNESI
                     YLLMR"
     gene            56328..57395
                     /gene="degS"
                     /locus_tag="OO544_000332"
     CDS             56328..57395
                     /gene="degS"
                     /locus_tag="OO544_000332"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HCR6766031.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQGGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
BASE COUNT        14264 a        15302 c        14253 g        13610 t
ORIGIN      
        1 cgcattattt tacaaaaaac acactaaact cttcctgtct ccgataaaag atgattaaat
       61 gaaaactcat ttattttgca taaaaattca gtgagagcgg aaatccaggc tcatcatcag
      121 ttaattaagc agggtgttat tttatgacga cgattctcaa gcatctcccg gtaggtcaac
      181 gtattggtat cgctttttcc ggcggtctgg acaccagtgc cgcactgctg tggatgcgac
      241 aaaagggagc ggttccttat gcatatactg caaacctggg ccagccagac gaagaggatt
      301 atgatgcgat ccctcgtcgt gccatggaat acggcgcgga gaacgcacgt ctgatcgact
      361 gccgcaaaca actggtggcc gaaggtattg ccgctattca gtgtggcgca tttcataaca
      421 ccaccggcgg cctgacctat ttcaacacga cgccgctggg ccgcgccgtg actggtacca
      481 tgctggttgc tgcgatgaaa gaagatggcg tgaatatctg gggtgacggt agcacctata
      541 aaggaaacga tatcgaacgt ttctatcgtt atggtctgct gaccaatgct gaactgcaga
      601 tttacaaacc gtggcttgat actgacttta ttgatgaact gggcggccgt catgagatgt
      661 ctgaatttat gattgcctgc ggtttcgact acaaaatgtc tgtcgaaaaa gcctactcca
      721 cagactccaa catgcttggt gcaacgcacg aagcgaagga tctggaatac ctcaactcca
      781 gcgtcaaaat cgtcaaccca attatgggcg tgaaattctg ggatgagagc gtgaagatcc
      841 cggcagaaga agtcacagta cgctttgagc aaggtcatcc ggtagcgctg aacggtaaaa
      901 cctttagcga cgacgtagaa atgatgctgg aagctaaccg catcggcggt cgtcacggcc
      961 tgggcatgag cgaccagatt gaaaaccgta tcatcgaagc gaaaagccgt ggtatttacg
     1021 aagctccggg gatggcactg ctacatatcg cttatgaacg tctgttgacc ggtattcaca
     1081 acgaagacac cattgagcag tatcatgcac atggtcgtca gttgggccgt ctgctgtacc
     1141 aggggcgttg gtttgattcc caggcgctga tgctgcgtga ctctctgcaa cgctgggttg
     1201 ccagccagat cactggtgaa gttaccctgg agctgcgccg tgggaacgat tattcaatcc
     1261 tgaataccgt ctcagagaac ctgacctaca agccagagcg tctgacgatg gaaaaaggcg
     1321 actcggtgtt ctcgccagat gatcgtattg gtcaattgac catgcgtaac ctggatatca
     1381 ctgatacccg agagaaactt tttggttatg ccaaaactgg cctgctctcc tcctctgcca
     1441 cttcaggcgt gccgcaggtg gagaatctgg aaaacaaagg ccagtaattc gacatcaacc
     1501 ctgcccttca ggggtggggt taccttgctt atcctctgta aactcctttc tgagcatcac
     1561 ataatcgaaa acatcatggt ttgcatccac aacccgtgag tctcctttat agtgtgcaat
     1621 cacttcctcc agcgttttcg gttgttccgg ctttgttacc cccatccacg cattaataag
     1681 gtaattattg taagctgtcg aaaagatgtt gttttccgtt gtacgatcca cgccatcccc
     1741 aagaacgggg ctataccaga taaacatcgg gacatgatat gcctgctggc tagcctccct
     1801 gcctccatga aaatagacgt tcttcttcgt agggtcacgt tccaggccgt ggtcggcaaa
     1861 atacatgacc gaggcgcggc gatcttttaa taattcaaaa acctgaccta gcaaactatc
     1921 tgtgtaatga atggagttgt catagcaggc atcctgatcg tcctgcggtt gaaacacggc
     1981 gctggattgc ggataggcgc tacaagccgg ttcatggctt ccatttaaat gaagaacaat
     2041 cagtttcttt tgctgcgtat tttgctgtaa tgcttgcgat aaatgcggca acaacaattc
     2101 atcaaatcct ctgacataaa ctgtttccat ggcgcgcatg gcgatactgg taactgctgt
     2161 accattctgc cgaaaagcgg attgcgagct tagccagaaa gtctgaaatc ctgcttgatt
     2221 agccatatta ataatgttgt cggggtaatt atgaatgtca tgactcaaaa cagaatcagc
     2281 agttaaagaa aggggaaccg acagcgcagt gtaaggtgcg ccgcttattg attgattaaa
     2341 cagtttgatc tgttttcttt gtgcttcaac ttgcggtgtc gtagagcgtg tatatccata
     2401 caaagacata ttgtcgacac gtacagactc ccccacaatc aacacgtagg tatcaatacc
     2461 tgtatccctg actgataatt gaaaatacgg caccgtgttt gcaattgaga gtaatctttg
     2521 atgctctttc gctgctaaag caaaataatt gagattgaaa aacggcgtat aggtagcaaa
     2581 tcgcgacgct agtatatatg gactgaatgc ttttttattt tttgcatctt tataagcaaa
     2641 ttgacaagca gaaaataaac tgcccgaaat gacaatcagc aataatattc ctgtcacttt
     2701 ttttgtcggc aaggaaacat catattttat tattactgcc aaaaaaagaa gggataaaaa
     2761 agcaaaagca catagataag ggatatacat ccccagcatt ttgacaactt catccggatc
     2821 actctgcagc acactaatcg caaatccatc attgaaagtt gtaccaaagg tacaccatga
     2881 atagaggcta atactcatgt ctgccgcagt cagtaacaca aatggtgcgg caacaagcag
     2941 gcgaagcgga agggatgata tacgttttac aaccaggatg gtcaggacga agaacagcaa
     3001 tcgctgaacc atatgcgcgc cggaagaggc gactaagttc gtaataccaa aaacaataac
     3061 acaaagatac aacaaccaat gagatttaaa atcttctagc aaatttgttg aatactgtca
     3121 tccagaaatc caagttcaca accaatataa acaattatca acaatgttca attttatatc
     3181 agacatgttt ttcaactctg atccatatca gaccgaaaca ctgttagttt tttcttttta
     3241 aatgatgcgg ccattgcgtt ctcagtaatt aaataacatt atcaatgcgt ctctaaacat
     3301 tacgcagaac gtagccatca gtagttatac tgacaggagc aggatggttt atcgagaagt
     3361 aaacatatcc gtattcgtac cggaactgga gttgacacaa taaagtgcca attatgtcag
     3421 cagaagggaa aagtttataa ctaaggcgta ccggcaccat cgtttcaagg taccagctac
     3481 gagtaaagca actggacgag atacagatac ctgacaacca ttcctcagac caggaccaaa
     3541 acgaaaaaag acgcttttca gcgtctcttt tctggaattt ggtaccgagg acgggacttg
     3601 aacccgtaag ccctattggg cactaccacc tcaaggtagc gtgtctacca attccaccac
     3661 ctcggcacgg atactacttt ttagttcggg atatcgctgg tcggcttagc cggagcagct
     3721 ggctgagttt gttcggtttt cgccggtgca ctcagatttt cccattcgct acctttattg
     3781 gttttgttgc tattgatgtt acccagcacc agactgatga taaagaataa cgttgccagc
     3841 agcgccgtca tgcgggtcat gaagttacca gaaccacttg aaccaaacag cgtagcggaa
     3901 gcgcctgctc cgaaggaggc tcccatatca gcgcctttac cttgctgcag catgatcaga
     3961 ccaacaaggc caattgccac aataaggaaa actaccaaaa gagcttcgta cataatcaac
     4021 ctgttccttg cggagttgcc gcgtaccaat gcttcaacca ataaagcggg agtttttaat
     4081 ctttcccact gaagtgggtg tgaatactaa ccaaagcgaa tgaccttcgc aagggcaatt
     4141 ttatcgcatt gtatcaactg cggaaaaaaa cagcaaaacc gatgtgttcg ctgagaaaaa
     4201 aggcgacaat cgccgccttt ttagccagtt atttaccgtt ttaaacggct tttactgcat
     4261 cggcgatacg gtgtgcaaat tcagttacct gcgcttcgtc ttcaccttct accatcacgc
     4321 gaattaacgg ttcggtgccg gatttacgca acagcacacg tccacggcta cccagcgccg
     4381 cttcaacttc ggcggtcaca gctttaactg actcatgctc aagcggatcg ccgctacctg
     4441 cggtgtaacg cacgttaacc agaatctgcg ggaacatttt catgccgctg caaaggtcgt
     4501 gcaggctcat atggttacgt gccatcgccg ccagcacctg caagccagca acgatgccgt
     4561 caccggtagt ggttttatcc agcaggatca catgaccgga attctctgca ccgatacgcc
     4621 agcctttctc ctgcattttt tccagtacgt agcggtcacc cactttcgcg cgcgcaaatg
     4681 gaattcctaa ctgtttcaac gccagctcaa gccccatgtt actcatcaat gtacccacag
     4741 caccaccacg cagctggccc tgacgaagac cttcacgcgc gatgatatac atgatctgat
     4801 caccatcgac tttattgcct tcatggtcaa ccataatcac gcgatcgccg tcgccgtcga
     4861 aggcaatacc gagatccgct ttttcagcca gcacacgagc ctggagcgcg cgaacgtcgg
     4921 tagcccccac ttcggcattg atgtttacac cgttcggctc acaaccgata gcgataacgt
     4981 tcgcccccag ttcacgcagc acgttcggcg cgatgtgata agtcgcaccg tttgcgcaat
     5041 ccaccacaat cttcagttcg ctgaggctaa gttcgttcgg gaacgtggct ttgcaaaact
     5101 cgatatagcg acccgcagca tcaacgatac ggctggcttt acccagttct gccgaatcaa
     5161 cacagctgat ctctttttcc atttccgctt cgatggcctc ttctaccgca tccggtagtt
     5221 tggtgccgtc gatagagaaa aatttaatgc cattatcgta gaacgggtta tgcgatgcag
     5281 atatcacaat tccggcctct gcgcggaagg tacgcgtcag ataagccacg gccggtgttg
     5341 gcatcgggcc agtgaagagt gcggaaaggc ccgctgccgc cagacccgct tccagtgctg
     5401 actccagcat atagccagaa atacgcgtgt ctttaccaat aatgatctta cgggagccgt
     5461 ggcgcgccag cactttaccc gcggcccaac ccagcttaag cacaaaatca ggtgtgatcg
     5521 gcgcatcccc tacacgacca cgaatcccat cggtaccgaa atatttacga ttactcatag
     5581 cgtttgtttt cctttgcaga cagagtggct tccaccaccc gcatcgcttc tacggtttct
     5641 ttgacgtcat gaacacgaat aatgtgcgcg ccttgcattg cggcaatgac cgcacaggcc
     5701 agactaccgc tcaggcgctc ggacggcccc acgttcagca gctgcccaat catcgatttt
     5761 cgtgacatac ctaccaacag cggcaggttg aaatggtgaa attcagccag gcgcgccagt
     5821 aatgaatagt tatgggagag atttttaccg aaaccgaatc cggggtcgag caacaatttc
     5881 tcttttgcga tacccgcctg ctcgcaacgt gctatttgct caataaagta gcgattcact
     5941 tctgcaaaga catcgtcata cttcggagct tcctgcatgg tttttggatt tccctgcata
     6001 tgcatcagac aaaccggtaa accggtttct gcagccgcct ccagagcgcc aggttcggaa
     6061 agggagcgga tatcgttaat aatgtgagcg ccaactttcg ctgactcacg gatgacttct
     6121 ggtttggatg tatctaccga gatccagact tcgaagcgtt gagcaattgc ctcaaccaca
     6181 ggaataacac gttgcaactc ttcttcaacg ctaacttccg ccgcccctgg gcgcgtggac
     6241 tcgccaccaa cgtcaatgat cgtcgcgcca gcgttgatca tcagattcgc atgtttcacc
     6301 gcatctatca gcgagttatg cgtgccacca tccgaaaagg aatcaggcgt gacgttgagg
     6361 atccccatta cgtgaggatg gctaaggtcc agtgaagtgc cctgggcaaa gagtttcatg
     6421 gtgttatccc tggtatgaat tgataagaaa aaaccccgga gcacgccccg gggttttcgg
     6481 tacaaataca gtcatctgat gcgggaactt acttgtcgcc taactgctct gacatggtgt
     6541 tacccgggtt cggcgtacgc ggttcatcaa ccggacgagg agcctttgga ctaccattgt
     6601 cgccagaatt gttagaagcg cctggttctt cccagcccgc tggcggacgt acatcgcgac
     6661 gtgccatcag gtcatcaatc tgcggtgcgt cgatagtctc atatttcatg agagcatctt
     6721 tcatcgcatg cagaatatcc atattgtcgg tcagaagctg acgcgcacga ttatagttac
     6781 gctcaatcag tgctttcact tcctggtcga tgatacgtgc agtttcatcg gacatatgtt
     6841 tcgctttcgc tacgctacgg ccgaggaaca cttcaccttc ttcttccgcg tacagcagtg
     6901 gacccaattt ctcagagaag ccccactgag tcaccatgtt acgcgccagg ttggtcgcaa
     6961 ctttaatatc gttggacgca ccggtagaaa catgttccgg cccgtagatg atctcttctg
     7021 ccagacgacc accgtacagc gtagaaatct ggctttccag tttctgacgg ctggcgctga
     7081 ttgcgtcgcc ctcaggcaag aagaaagtca cacccagcgc acgaccgcgt gggataatcg
     7141 tcactttgtg caccggatcg tgttccggca ccaggcgacc gataatcgca tgacccgctt
     7201 cgtggtaagc cgtcgattct ttctgcgctt ccgtcatcac catggagcga cgttccgcac
     7261 ccatcatgat tttatctttc gctttctcga actcaaccat cgacacaacg cgtttgttgc
     7321 cacgagcagc gaacagtgcc gcttcgttta ccaggttcgc caggtcagca ccggagaaac
     7381 caggagtacc acgggcaatg attgccgcgt cgatatcggg tgccaatggt acgcgacgca
     7441 tgtgaacttt caggatctgc tcacgaccgc gaacatctgg caagccaacc acaacctgac
     7501 ggtcgaaacg gccaggacgc agcagggccg ggtcaagaac gtccggacgg ttagtcgcgg
     7561 cgataacgat gataccttcg ttaccttcga agccatccat ctcaaccagc atctggttca
     7621 gagtctgttc acgttcatcg tgaccaccgc ccagaccagc gccacgctgg cggcctacgg
     7681 cgtcgatttc atcgataaag atgatgcacg gtgccgcttt cttcgcctgt tcgaacatgt
     7741 cacgaacacg ggatgcaccc acaccgacga acatttctac gaagtcagaa ccggagatag
     7801 taaagaacgg aactttcgct tcgcctgcaa tcgctttcgc cagcagcgtt ttaccggtac
     7861 ccggaggacc gaccatcagg acgcctttcg ggatcttacc gccgagtttc tggaagcggc
     7921 tcggctcgcg cagatattca accagttcag caacttcttc ttttgcttcg tcgcagcccg
     7981 caacgtcagc aaaggtcgtt ttgatctgat cttccgtcag catgcgcgct ttgctcttac
     8041 caaacgacat ggcacctttg ccaccgccgc cctgcatttg acgcatgaag aagatccaca
     8101 caccaatcag caacagcatc gggaaccagg agatgaagat agaagccagc aggcttggtt
     8161 cttcaggcgg ttcaccgaca accttgacgt tcttggtcaa caggttatcc agtaatttcg
     8221 gatcctgaac cggaatgtaa gtggtataac ggttactatc tttcttggta acgttgattt
     8281 cacgtccgtt gatacgcgct tcacgaacct ggtcgttatt gacctcttgt aggaaggtag
     8341 agtaatccac cttacggcca ttagactcgc tgggcccaaa gctctggaat actgacatca
     8401 gcacaacggc aatgaccagc cagagtatta ggtttttcgc catgtcactc aagggattaa
     8461 cctcttatta caactgtgtt aaaaacagcg tcaggatact ctatatccag catctttcaa
     8521 actttcgtct gaaatctccc ggttagggtt tacgcccggt cgctacaata tacacttccc
     8581 gcgaacgtgc acgagaagag tccggcttac gaactttgac cttcgtaaac agggagcgaa
     8641 tttcccttag atactcatcg aaaccttcgc cctggaacac cttcactaca aaactgccac
     8701 ctggcgctaa tacatcacga cacatttcta gcgccagttc caccagatac atggcacggg
     8761 ggatatccac cgccggtgtt ccgctcatgt ttggtgccat atcggacatg acaacctgga
     8821 ctttgctgtc gccaacgcgc tccagcagtg ctttcatcac cagttcatca cgaaaatcgc
     8881 cctgaagaaa gtctacacca acgataggat ccataggtaa aagatcgcaa gcgatgatgc
     8941 ggcctttgcc gccaatttgg gtgaccacat attgtgacca accacccgga gcagcaccaa
     9001 ggtcgacaac cgtcattccc ggtttaaaga gtttgtcact ttgctgtatt tcatcaagtt
     9061 taaaccaggc acgggaacgt aacccctttt tctgtgcctg ttgaacatat ttatcgctaa
     9121 agtgttcctg aagccagcgg ctggagctgg cagaacgctt cttacctgtc atttaacttt
     9181 cccatgggga taactcatcg taaccaattg cgtaaatttt tacgcgccta tttggtgata
     9241 tatgggagat ggcggtagaa tgacccattt tcaatcccaa cgtaagcaaa aaatacgatg
     9301 aatctgagta ctaaacaaaa acagcacctg aaaggtctgg cacatccgct caagccagtt
     9361 gttctgcttg gcagtaatgg tttgaccgaa ggggtgctgg ccgagattga acaagcgtta
     9421 gagcaccatg aactcatcaa ggtgaaaatc gccaccgaag atcgcgaaac aaaaaccttg
     9481 atcgtggaag ctatcgtgcg cgaaaccggc gcctgtaatg tacaggtcat cggtaaaacg
     9541 ctggtgcttt atcgcccaac taaagaacgt aaaatctcgc tgccacgcta agattatcct
     9601 aaagttacac acatttgctg tgtaaaacga ggggttttcc gcaggcagga gagcaaaatg
     9661 ccacgctctg ttcgttgata aaaggccgca tagcggcctt tttcctttct ttacaataca
     9721 tcaacatctt gagtattggg taattcttac aggtattcca ccttaattac ttcaaattct
     9781 acttcgccgc ccggcgtttt gatgaccaca acatcatctt cttctttgcc gatcaggcca
     9841 cgagcaatag gcgagtttac agaaatcagg ttttgtttaa agtcagcttc gtcatcgcca
     9901 acgatgcgat aagtctgttc ttcgtcagaa tccagattca gcaccgttac ggtagcacca
     9961 aaaataacgc gcccattgtt aggcattttg gtgacatcaa tcacctgcgc gtttgacagc
    10021 ttggcttcga tgtctttgat acggccttcg cagaagccct gctgttcacg agctgcgtgg
    10081 tattcggcgt tttctttcag gtcgccatgc tcacgcgctt ccgcgatagc agcaatgatt
    10141 tcaggacggc gcacagattt cagaaaatcc agctcttcgc gtaatttttc agcgccgcgt
    10201 aaggtcatcg gaatagcttg catttgttat acctcttgaa tattcctgat ggggcaagtc
    10261 ttcacccacc ccggctgttc ggcctgcccg gcataactcc tcaccgggac cagaagcaaa
    10321 aaaataccga cccaggtaca agtcccaggt cagctacaat tcacattttg atagtcattt
    10381 taccctgaag ttcccgaagg gtcatcgttt actttatagg gcgttgcgcc gtagtatgac
    10441 ggctcgattc caggttgtta gcgcgagatt atgcgatttt ccagatttat catcggattg
    10501 accagctgta tagcgttcag tgttcaggcc gcaaatgttg atgagtacat tactcaactc
    10561 cccgctggtg ccaaccttgc cctgatggtg caaaaagtcg gcgcgtcggc ccccgctatt
    10621 gattaccaca gtcagcagat ggcgctgcct gccagtaccc agaaagtgat tactgcgctg
    10681 gcggcgttga ttcaactcgg ccccgatttt cgttttacca cgacgcttga aaccaaaggc
    10741 aatgtggaaa acggcgtact taagggtgac ttagtggcgc gatttggtgc cgatccgacg
    10801 ttaaaacgtc aggatattcg caatatggtc gcgactttga aaaaatctgg cgtcaaccaa
    10861 atcgatggca atgtgttgat agatacctcc attttcgcca gccacgataa agcccccggc
    10921 tggccatgga atgacatgac acaatgcttt agcgctccgc ctgccgccgc catagttgac
    10981 cgcaactgtt tctccgtctc gctctacagt gcccaaaaac ccggtgatat ggcctttata
    11041 cgcgtggcat cttattaccc cgttacgatg ttcagccagg tacgcaccct cccccgtggt
    11101 tctgccgaag cgcaatactg cgaactggat gtcgtaccag gcgacctgaa ccgctttacg
    11161 ctgacgggat gcctgccaca acgttctgag ccgctcccgt tggcttttgc cgtgcaggat
    11221 ggagccagtt atgccggtgc aattctgaaa gatgagttaa aacaggcggg tatcacctgg
    11281 agcggaacac tgctgcgcca gactcaggtt aacgaacctg gaacggtagt tgccagtaaa
    11341 cagtcggccc cgctgcacga tctgcttaag attatgctga aaaagtcgga taacatgatc
    11401 gccgatacgg ttttccgcat gataggccat gcgcgcttca atgtacctgg tacatggcgg
    11461 gccgggtcgg acgctgtgcg tcagatcctg cgccagcaag ccggtgtcga tattggaaac
    11521 accattattg ccgatggttc agggctttcg cggcataacc tgattgcccc cgccaccatg
    11581 atgcaggtgc tgcaatacat tgcccaacac gacaatgaac ttaactttat ctccatgctg
    11641 ccgctggcgg gctatgacgg ctctttgcag taccgtgcag gtctgcatca ggcgggcgtg
    11701 gatggaaaag tctcagcgaa aaccggttcg ttgcaggggg tatataacct ggcgggattc
    11761 attaccacag cgagcgggca acgaatggcg tttgtgcaat atctttctgg ctatgcagta
    11821 gaacctgcgg atcagcgtaa tcgccgtatt ccgttagtgc gttttgaaag ccgtttgtat
    11881 aaagatattt atcagaacaa ttagtcaaaa agaaaccccg gcacatggcc ggggcttcag
    11941 attattgaca aagtgcgctt tgtttatgcc ggatgcggcg taaacgcctt atccggccta
    12001 caaaatcgtg caaattcaac atattgcaat tctcttgtag gcctgataag cgtagcgcat
    12061 caggctgatt tggcgtttat catcagtgat taacgcttgt aaatgaactc aacgccttct
    12121 tcgtcgtctt cgtcccagtc gtcatcccag tcttcatcat cctcttcagc aatctcttca
    12181 agctgctggc gatgataatc gtcccacatg aattcgactt tctctggctg tttcgcttct
    12241 tcagcctgca cgaccgggtt ttcaaggata aaggtcatca catcccagca gagatctttc
    12301 acgcccagtc cgctcgccgc agagatcaga taatatttat cttcccagcc cagcgcttca
    12361 gcaatcgctt tcgctttctc ttcagcttct gccttatcca gcagatcgat cttattgaac
    12421 actaaccaac gcggtttcgc cgccagatcc tggctgtatt tttccagctc gctgataata
    12481 atacgcgcgt tttcaaccgg atcggtgccg tcaatcggat cgatatcgat gaggtgcaac
    12541 aggacgcggc aacgttccag gtgcttcagg aagcggatac ccagacctgc gccttccgca
    12601 gcgccttcaa tcagtcctgg aatatcggca acaacgaagc tcttttcgtt gtccattcgt
    12661 accacaccca gacttggcac cagagtggta aacggataat ccgccacttt cggtttagcc
    12721 gccgataccg cgcgaataaa ggtcgattta cccgcgtttg gcatccccaa cataccgacg
    12781 tcagccagca gcatcagctc cagcagcagc tcacgcttat cacccggcgt accgttggtt
    12841 ttctgccgtg gtgtacggtt aacggacgat ttgaaacggg tattgcccag accgtgccag
    12901 ccgcccttag caaccagcag acgctgaccg tgtttagtca tatcgcccat ggtttcacca
    12961 gtaccctggt cgattacacg cgtacctacc ggcactttaa tcgtcacgtc tttaccgcgc
    13021 ttaccggtac aatcgcggct tgcgccgttc tgaccgcgct ctgcacggaa agatttttca
    13081 aaacgataat cgataagcgt gttcaggttt tcgtcggctt ccatccatac gtcaccacca
    13141 tcaccaccgt cgccgccatc cgggccgcct ttcggaatat acttttcgcg gcggaagctc
    13201 acgcaaccat taccgccatc acctgcaacg accagaatcg atgcttcatc aacaaacttc
    13261 attttattct ccataaatca ttcgcctgcg cggggttgcg ataccaccgt tgtatgctta
    13321 cgtaatccgc cccaaatacg atgaccaatg gcggaataca tcgcgcccgc aaccacgaca
    13381 aacgcaccga gataacctaa aaggtttaac atcggtctgg cgaagaaatc gggccaggcc
    13441 agtgataaaa gatctgaaaa aaacagcgta aacagtgggg tgagcgtgat gatcgcgctc
    13501 acctgcgctg cctgccagcg agccatcgct tccgccaggg cgccatatcc taccaatgta
    13561 ttcagtccgc aaaaaattaa acatgcgagc tgccagtggc taagctgcgc tatcactcca
    13621 ggctttgcca gagggaagag cgcaattgta cataaagtgt acagtaaaaa caggatctgc
    13681 ggtgaggcca gccgacgcaa taaaaccttt tgcgccacgc cataactcac ccaaaccgtc
    13741 gccgcaccga ccccaaagat aactccccag gtgtaatcgg tgagctttgt aaatatctcg
    13801 accagactgg tgttaaaaaa catcaccagg ccgctcagga gcatcaatgc ccctacaacc
    13861 tgagtgctgc gcattttctc tttcaggata aatacgctgg caaccatcat gccaactggc
    13921 gagagttgcc caatcacctg cgaagcggtc ggactcaggt attgcaagga tgagctgaac
    13981 aggatgaagt tcccaaacag cccggcggtc gccactgcca acaaaatcaa ccagcgtggc
    14041 ttacgaaaca cgcgtaatgg cggcaacctc ttcttcaccg caagaatggc accaaggcca
    14101 atactcgcca tcaagaaacg gtaaaacacg attgtcggag gttccatcac ctccagcacc
    14161 tgcttcattg cgattggcaa cgccccccag caaattgctg tggtgagcgc caaaagaatg
    14221 ccaatgcctg cctgctgctt cataccgtat tccctgcaaa gcgcatttgc ggattaacgt
    14281 cgcaccatgc gcatttttac cggttatcga atgtaaaaag ccccgcaacg tgttgcgggg
    14341 ctttcatccg ttaccgggac gcgaaaaact tattcagctt cgatgctgat aaatttacgg
    14401 tttttcgggc ctttaacttc gaatttcact ttaccgtctg ctttagcaaa cagagtgtgg
    14461 tcacgaccgc aacctacgtt agcgccagcg tggaatttgg taccacgttg acgaacgatg
    14521 atgctacccg ccagaacgga ttcgccaccg aaacgcttaa cgcccaggcg tttagcttct
    14581 gaatcgcgac cgttacgtgt ggagccgcca gcctttttat gtgccatttg aaatctctcc
    14641 tcaggtctta ggcgctgatg ccagtaattt tcacatcagt gaaccactga cgatgaccct
    14701 gctgcttacg atagtgttta cgacgacgaa acttaacgat tttaactttc tcgccacgac
    14761 cgtgagcaac aacttcagct ttgattacgc cgccatcaac gaaaggaacg ccgattttga
    14821 cttcttcacc gtttgcgatc atcagcactt cagcgaactc aacagtttcg ccagttgcga
    14881 tgtccagctt ttccaggcga acggtctgac cttcgcttac tcggtgttgt ttaccaccac
    14941 tttggaaaac cgcgtacata taaaactccg cttccgcgca caccttttca atgattcaga
    15001 gtgcgctata aatattcaca atagggcgcg aatattacgc aaaacgcacg cctttgacaa
    15061 gtgctacagt caatacacga agaaaaaaaa cacaacttgt acggtaacgt ttatctgtgc
    15121 cattttttca gtacaatcac cctatattcc taaccataaa ccctaagttg cctttgttca
    15181 cagtaaggta atcggggcga aaagcccggc ttttgcgatg aatttagaaa aaatcaatga
    15241 gttaaccgcg caagatatgg cgggtgttaa tgcggcaatc cttgagcagc ttaattccga
    15301 cgtccaactg atcaatcagt taggctatta catcgtcagc ggcggcggta aacgtattcg
    15361 tccgatgatt gctgtactgg ctgcacgagc tgttggctat gagggaaatg cgcatgtcac
    15421 cattgctgcc ctgatcgagt ttatccacac ggcgactctg ctacacgacg acgttgtgga
    15481 tgaatcagat atgcgcaggg gtaaagctac cgccaacgcc gcatttggca atgccgccag
    15541 cgtgctggta ggcgatttta tttatacccg cgcgttccag atgatgacca gcctcggttc
    15601 actcaaagtg ctggaagtca tgtcagaagc tgtaaacgtc atcgcagaag gtgaagttct
    15661 gcaactgatg aacgttaacg atccggacat cactgaagaa aactacatgc gcgttatcta
    15721 tagcaaaacc gcgcgtctgt ttgaggctgc cgcgcagtgt tccgggattc tggctggctg
    15781 tacgccggag gaggagaaag gcctgcagga ttatgggcgc tatctcggca ctgctttcca
    15841 gttgatcgac gatttactcg attacaatgc cgatggcgaa cagttaggta aaaatgtcgg
    15901 cgacgatctg aacgaaggta aaccgacgct gccgctgctg catgcgatgc atcatggcac
    15961 accagaacag gcacagatga tccgtaccgc catcgaacag ggtaacggtc gccatcttct
    16021 ggaaccggtt ctggaagcaa tgaacgcttg tggatctctt gaatggacgc gtcagcgtgc
    16081 cgaggaagaa gcagacaaag ccatcgcagc attacaggtg ctcccggaca ccccttggcg
    16141 agaagcactc atcggcctcg cgcacatcgc tgttcaacgc gatcgttaat cccctccctt
    16201 catcccgcgc agtgcgcggg atgattccag taaattccat aaaaacacta attcagcgct
    16261 ataacacctg aaaaaaaagg gcgatgaatc tttatatatt ctgaatattc acactcttta
    16321 caggaacttt ttagagcaat aggccatcag gagtatagtg atgcccgaca gaagaagtgt
    16381 tctgaatgaa agcgactaac ttaaggagtg aggaaagatg gaaagtaatt tcattgactg
    16441 gcatcccgct gacatcattg cgggtttgcg caagaaggga acgtcaatgg cggcggagtc
    16501 tcgcagaaat ggtttgagtt cctcaacgct ggcaaacgca ttatcgcgcc catggccgaa
    16561 aggagagatg attattgcga aagccctggg aactgacccc tgggttatct ggccatcacg
    16621 ctaccatgat ccgcagaccc atgagtttat cgacagaacg cagttgatgc gtagctacac
    16681 taaaccgaaa aaatgagtgg tctggcggta gccccgcgaa cggggctgcc agctctcaga
    16741 cgattactcg cctttcacac gctcaatatt tgcacctaaa gcgcgcagtt tgtcttcaat
    16801 gcgttcgtag ccacgatcga tgtgataaat acgatcaacc accgtcgtcc cttccgcaat
    16861 acagccagcc agcaccaggc ttgctgatgc acgcagatcg gttgccataa cctgtgcgcc
    16921 agaaagtttt tcaacaccgt gacaaataac ggtattgctt tcgatttcgg catgcgcgcc
    16981 catacggcta agctccggca catgcataaa gcggttttca aagaccgttt cggtgatgaa
    17041 tccggtccct tctgccacca ggttcaacag cgtgaactgg gcctgcatat cggtcgggaa
    17101 tgccggatgc ggcgcggtac gtacgttaac agccttcgga cgtttgccat gcatatccag
    17161 gctaatccag tcttcgccga cttcgatgtc cgctccagcg tcacgcagtt tcgccagcac
    17221 ggcgtcgaga gtatctggct gcgcgttacg gcagataatt ttgccgcgag aaatcgccgc
    17281 cgccaccagg aaagtaccgg tttcgatacg atccggtagc acgcgataga caccgccgcc
    17341 taaacgttcc acaccttcga tgacgatacg atcggtgccc tgaccgctaa ttttcgcacc
    17401 cagcgtaatc aggaagttcg cggtatcgac gatttccggt tcacgcgctg cattttcaat
    17461 aatcgtggta ccttccgcca gggttgcagc acacatgatg gtcaccgttg cgccaacgct
    17521 gactttatcc atcacgatat gtgcgccttt cagacgacca tcgacggaag ctttaacgta
    17581 accttcttcc agtttgatgg tcgcgcctaa ttgttcgagg ccagaaatgt gtagatcaac
    17641 cggacgcgca ccgatcgtac aaccgccagg cagtgaaact tgcccctgac caaagcgcgc
    17701 taccagcggc cccagtgccc agatagaagc acgcatggtt ttaaccagat cgtaaggtgc
    17761 gcagaataca ttaacgtcgc gggcatcaat atgcacagaa ccattacgtt ctactttcgc
    17821 acccagctgg cttagcagct tcattgatgt atcgacgtct ttcagttttg ggacgttctg
    17881 gatctctacc ggttcttccg ccagtagagc ggcaaaaagg ataggcagag cagcattttt
    17941 agcgccggaa attgtgactt cgccctggag cttcgttggc ccctgaacac gaaatttatc
    18001 catttagttt gttctcagtt aacaattcat atccgctacc ggcgaatcgc ccatagctca
    18061 aaagccgttc agtttgcgat cgcgcgccca ctccgcaggg gtatacgctt tgatcgacac
    18121 agcatgaatg cggttatccg caatatattc catcagcggg ccatagaccg tctgctgttt
    18181 tttaacccga ctcatgccgt caaacaactc acccacggca ataacctgaa agtggctgcc
    18241 atcgccggaa acgtggactt cctggaggga gagagcgttc atcaacacgc tctgaatttc
    18301 attattttcc atgggatctt caatcatcag ttaataaacc agcgaaacat cttagagcaa
    18361 agttgcgctg gcataaataa gcaaaaagcc tcgctgataa atcagacaag gctcgacttg
    18421 caggcaggtt tgccggacag gcggttaacg ccatatccgg cctgaaaaaa tttaacgagg
    18481 cagaacatca gcaggcaaat tatacaattt cgcgagggta tacactttgt cgtttacccc
    18541 ctgaagcgtc acgttgttgc cctgcttttt cgccagatcg ataagatgga gcagcagtgc
    18601 cagtccaccc gtatccacgc gggacacacg gctaagatcg atgcaggtaa tccccttcac
    18661 cgcttcctca cgcatttccc aaagcggtag cagaacgtcc tgatccagct ctccggataa
    18721 cgccagcgtg tcacccgtct gcatccagct cagtgactcg ctcattattt tttctcttcc
    18781 agagtgattt tctgttgaga aatcgatttc agttgcgcag tcaggccgtc gatacctttg
    18841 gtacgcagca gcgttcccca ctcgttttgt ttggtggtga tcatactgac gccttcagca
    18901 atcatgtcgt aagcctgcca gtttcccgtc tgggagtttt tacgccactg gaagtccaga
    18961 cgcaccggcg gacggccatt cgggtcaata atggtaacgc gaataggcac aatggtttta
    19021 tcgcccagcg gctgttctgg cgcaatctga taggtttgac cgtgatacat cgccagcgcc
    19081 tgaccgtaag cctgcttcag gtactcacgg aaagcggcaa agtaggcttc acgttgagca
    19141 ggggtcgcac tcttgtaata ctggcccagc accagcgcac cggcgtattt cacctgtacg
    19201 tatggcagca gttcctgatc aacaatggta cgcagataat ccgggttggc ccgaatttgc
    19261 ggttgctcat tcttcaggcg atcgaacgtt ttctgcgccg cctcgtccat cagcttataa
    19321 ggattggtct ggtctgccgc ggttgccgca ctcagaggtg caatcaccag caaagcgacc
    19381 atcattaaac gtttaaacat gcgtcggttc tcctgaaatt atttcgttgt acccacaggt
    19441 tcagtggttt cattattacc tggcgcagca gctggcgcat cgccactatt cttattgtca
    19501 tcgcctttac taccgtaaag gaactgacca atgagatctt ccagcaccat cgcagactta
    19561 gtgtcctgaa ttgtatcgcc atccttcagg atagcagtcc ccagttccgg gtcttcaaaa
    19621 ccgacgttta atgccagata ttgttccccc agcaggccgg aagtacgaat gctcagcgaa
    19681 ctggtatctg gaatgtggtt ataacgttgt tcaatttcca gcgttacgcg cggcagatag
    19741 gttttcgggt ccagcgtaat atccgccacc cgacccacaa caacgccacc aatactgacc
    19801 ggagagcgcg ctttcaggcc gccaatgtta tcgaacgtcg cataaagcgt gtaggtcggt
    19861 tcagtacgta tggacgtcac gttcgccgcc ttcaggcaaa caaacagcgc cgccagcagt
    19921 gctgctaata aaaagatacc cacccaaatt tcattttttt tcgtttgcat gaactcaatt
    19981 cccaaacatc aatgcggtca gcacaaaatc cagccccaga acagccagag acgagtggac
    20041 aacggtgcga gtggttgccc ggctaatccc ggcagacgtc gggatggtgt cgtagccgtt
    20101 aaacaacgaa atccacgtca ccgtgatggc gaacaccacg ctcttaatca gacagttgac
    20161 cagatccata cgccagtcga cggcattttg cattgccgac cagaagaacc cgctatcaat
    20221 gcctttccaa ctgacgccga ccagagatcc gccccagatc cccacggcga cgaaaataac
    20281 cgtcaacagt ggtaatgaaa taaccccagc ccagaaacgg ggagaaataa cccgacgcag
    20341 cggatccacc gccatcatct ccatactgga gagttgctct gtagcgcgca tcaggccgat
    20401 ttctgcggtt agcgccgaac cagcacgccc ggcaaacaac aacgcggcaa ccaccggccc
    20461 cagttcacgc agtagcgata acgccaccag catacccaga ctggtttccg cactataagt
    20521 ggtcagaacc agataacctt gcagccccaa caccattccg atgaacacgc cagaaaccac
    20581 aataatcagc atcgacagga cgccgacatt atagagctgg cgcaccagca gcggcgcatg
    20641 tttgcgaaat tccggtttgc cgaccagcgc attgaataac attaacccgg cccgcccgaa
    20701 cgttctcagg gttttaatcc ctttatgtcc gagcgacgcc agcgcattta acagcatgag
    20761 tggcttaact ccctggtaaa agatcagcgt gataatcgcc ggcaggatag cggaacggaa
    20821 caggcccgtc agctatcccg tccagaaact gacgtacgcg cggatcagga ttcgcctgca
    20881 acgcctgggc actgccatga gcgacaattt ttttgtccgc caggatccag gcgtgatccg
    20941 caatacttaa cacttccggc acatcgtgag aaaccaccac acaagtcacg cccagcgcgc
    21001 tgttcagctc agaaatcagc ttcaccagta cgcccatggt gatgggatct tgcccaacaa
    21061 aaggttcatc aaacatgatg agatccggct ccagcgcaat cgcacgcgcc agcgctgcac
    21121 gccgcgccat cccaccggaa agttcagaag gcattagttt agccgctcca cgcagcccca
    21181 cggcctccag cttcatcatc accgtactat gcaacaatgg cgcgggtagt tgggtatgtt
    21241 cgcgcagtgg ataggcgacg ttgtcaaata cgttcatatc agtgaacaac gccccggact
    21301 gaaataacat gctcatccgt ttgcgcactg tatacaggcg cgaacgagac atcgccggaa
    21361 tattctcacc atcgaaaagg atctcaccat gatctggtgc gatttgcccg ccaatcagac
    21421 ggagtagcgt cgttttaccg atgcccgatg gccccatgat cgccgtgatc ttccctcgcg
    21481 gcacggtcag ggaaatatta tcgaagatgc agcgattgcc acgcgtaaaa ctgacatcgc
    21541 gcatatcgac taaattcgcc acagactgct ccataattca cccttcgtct tgcgttgatt
    21601 ttctaagcat ggcgctcaat ttaaccttga acccaacata tttacagaat attacccgcc
    21661 gtggttagcg aaagctggca tttgttttac tttttagccg cataaagtca aaattaagaa
    21721 tccgttacgg ctttctgaaa atcttcagcg gaccgacgag tatacctgaa gaaaggacgt
    21781 tagatgcttt tagctacggc actgttaatt gttggtttac ttttggtcgt ttacagtgcc
    21841 gaccgcctgg tttttgccgc gtctattctt tgccgaacct ttggcatccc gccgctgatc
    21901 atcggcatga cggtggtcag tattggtaca tcgttaccag aaatcatcgt ctcgcttgcc
    21961 gcgtctctgc acgaacaacg cgatttagcc gttggtacag ccctcggctc aaacattatc
    22021 aatattttgc tgatcctcgg tctggctgcg ctggttcgtc cttttaccgt ccattccgat
    22081 gttctgcgtc gtgaattacc cttaatgttg ttggtcagcg tagtggccgg ttccgtactc
    22141 tatgacggac aacttagtcg cagcgatggt atctttctcc tctttctggc tgtgctatgg
    22201 ctgctgttca ttgttaaact tgcacgtcag gctgaacgtc aggggactga cagcctgacc
    22261 agagagcagc ttgcagagct gccgcgtgac ggcggattgc ccgtcgcgtt tttatggctc
    22321 ggcattgcgc ttatcatcat gccagtggcc acgcggatgg tggttgataa cgccacggtg
    22381 ctggcgaatt actttgccat cagcgagttg acgatgggtc tgacggcaat tgctatcgga
    22441 accagcctgc cggaactggc aaccgcaata gcgggggttc gcaaaggtga aaacgacatt
    22501 gctgtcggaa atatcattgg cgcaaacatt tttaatattg tcatcgtgtt gggtttaccc
    22561 gcgctgataa cgccaggaga gattgagcca ttggcgtaca gtcgtgacta cagcgtgatg
    22621 ttgctggtga gcattatttt tgcgttgctg tgctggcggc gctccccgca accgggccgt
    22681 ggtgtagggg tattattaac tggcggattt atcgtatggc tggcgatgtt gtactggtta
    22741 tcgccaatac tcgttgaata actggaaacg cattatgtcg cacgtagagt tacaaccggg
    22801 ttttgacttt cagcaagcag gtaaagaagt cctggcgatt gaacgtgaat gcctggcgga
    22861 gcttgatcaa tacatcaatc agaatttcac gcttgcctgt gaaaagatgt tctggtgtaa
    22921 agggaaagtt gtcgtcatgg ggatggggaa atcggggcac atcgggcgca aaatggccgc
    22981 aacgtttgcc agcaccggta caccttcatt tttcgtccat cctggtgaag ccgcgcatgg
    23041 tgatttaggc atggtcaccc cacaggatgt ggtgattgct atctctaact caggtgaatc
    23101 cagcgaaatc acggccttaa ttccagtgct taagcgtctt cacgtaccgt taatctgcat
    23161 caccggtcgc ccggagagca gcatggcgcg cgccgcagat gtgcatctgt gtgttaaagt
    23221 agcgaaagaa gcctgtccgt tagggctggc accgaccagc agcaccaccg ccacgctggt
    23281 tatgggcgat gccctcgctg tcgcgctgtt aaaagcacgc ggctttactg ctgaagattt
    23341 tgcgctctca cacccaggcg gcgcactggg tcgtaaactt ctgctgcgcg taaacgatat
    23401 tatgcatacg ggcgatgaga tcccgcatgt taagaaaacg gccagtctgc gtgacgcgtt
    23461 gctggaagtt acccgcaaaa atcttggtat gactgtcatt tgcgatgaca atatgatgat
    23521 tgaaggcatc tttaccgacg gtgatttacg ccgtgtcttc gatatgggcg tggatgttcg
    23581 tcagttaagt attgccgatg tgatgacgcc ggggggaata cgtgtgcgcc ctggcattct
    23641 ggccgttgag gcactgaact taatgcagtc ccgccatatc acctccgtga tggttgccga
    23701 tggcgaccat ttactcggag tgttacatat gcatgattta ctgcgtgcag gcgtagtgta
    23761 aagattcaag gataaacaac aatgagcaaa gcaggtgcgt cgcttgcgac ctgttacggc
    23821 cctgtcagcg ccgacgttat agcaaaagca gagaacattc gtctgctgat cctcgatgtc
    23881 gatggcgtac tgtcagatgg cctgatttat atgggcaata atggcaaaga gctgaaagcg
    23941 ttcaatgttc gtgacggtta tggcattcgt tgtgcgctca cctctgatat tgaagtcgct
    24001 atcattaccg ggcgaaaggc taaactggta gaagatcgtt gtgccacatt ggggatcact
    24061 cacttgtatc aggggcagtc aaacaaactg atcgccttta gcgatctgct ggaaaaactg
    24121 gcgattgccc cggaaaatgt ggcttatgtc ggcgatgatc tcatcgactg gccggtaatg
    24181 gaaaaagtgg gtttaagcgt cgccgtggcc gatgcgcatc cactgttgat cccgcgcgcc
    24241 gattacgtga cgcgcattgc tggcggtcgt ggcgcagtgc gcgaagtttg cgacttatta
    24301 ctcctggcgc agggcaaact ggatgaagcc aaagggcaat cgatatgagt aaagccagac
    24361 gttgggttat cattgtgcta tcactggcgg ttctggtgat gatcggcatt gatatggccg
    24421 aaaaagacga taccgcccag gtggtcgtca acaacaatga tcccacctat aaaagcgagc
    24481 atacggacac gctcgtctat aacccagaag gggcactaag ctatcgattg attgctcaac
    24541 acgttgaata ttattccgat caggccgttt cgtggtttac gcagccggta cttaccacgt
    24601 ttgataagga taaaatcccg acatggtccg taaaagcaga taaagccaag ctgaccaatg
    24661 accggatgct ctatttatat ggacacgttg aagtcaacgc acttgtgcca aactctcaac
    24721 ttcgcagaat cacgacggat aacgcgcaga tcaatctggt gacgcaggat gttacctctg
    24781 aagacctcgt cacgttatac ggaacaacat ttaactccag cggtctgaaa atgcgcggca
    24841 acttacgcag caagaacgcc gagctgattg aaaaggttag aacatcctat gaaattcaaa
    24901 acaaacaaac tcagccttaa tcttgtgctt gccagctcac ttctggccgc cagcattccg
    24961 gcatttgccg taaccggaga cactgatcag ccgatccaca ttgaatcgga ccagcaatct
    25021 cttgatatgc aaggcaacgt ggttaccttt accggtaatg tcatcgtcac ccagggcacc
    25081 atcaaaatta atgccgacaa agtggtcgtt acccgtccgg gcggcgaaca aggtaaagaa
    25141 gtgattgacg gctacggtaa accggcaacg ttctaccaga tgcaggacaa cggtaaaccc
    25201 gttgaaggtc acgcttccca gatgcactac gaactggcaa aagattttgt cgttctgacg
    25261 ggtaatgctt atctgcagca ggtcgatagc aacattaagg gcgataagat cacttacctg
    25321 gtaaaagagc agaaaatgca ggctttcagc gacaaaggca agcgcgtaac caccgttctg
    25381 gtgccgtcgc agctgcagga caaaaacaac aaaggccaga ccccggcaca gaagaagggt
    25441 aattaattcg ttatggcaac attaactgca aagaaccttg caaaagccta taaaggccgt
    25501 cgcgtggtag aagacgtcag cctgaccgtc aactccgggg aaattgtcgg tctgctgggg
    25561 ccaaacggtg ccggtaagac caccactttc tacatggttg taggcattgt gccgcgcgat
    25621 gcgggcaaca tcattattga tgatgacgat atcagtctgc tgcctctgca tgcacgcgcg
    25681 cgccgcggta tcggctatct gccacaggaa gcctccattt tccgtcgcct cagcgtttac
    25741 gataacctga tggcggtact gcaaattcgt gacgacttgt ctgctgaaca acgtgaagac
    25801 cgcgcgaacg agctgatgga agagtttcac attgagcacc tgcgtgacag catggggcag
    25861 tcactctccg ggggtgaacg tcgccgtgta gaaattgccc gcgcactggc tgcgaatccg
    25921 aaatttattc tgctcgacga accgtttgcc ggggttgacc cgatctcggt tatcgacatt
    25981 aaacgcatca ttgagcacct gcgcgacagc ggcctgggcg tgctgatcac tgaccacaac
    26041 gtgcgtgaaa cactggcggt ttgtgaacgc gcttatatcg tcagtcaggg gcatttgatc
    26101 gcccacggca cgcctacaga aatcttacaa gacgaacacg ttaagcgtgt ataccttggg
    26161 gaagacttca gactctgata gggtagaagt ttgcgacgtt ttagcaggag agtacgattc
    26221 tgaacatgaa gcaaggtttg caactcaggc ttagccaaca actggcgatg acgccacagc
    26281 tccaacaggc aattcgtctg ttgcagttgt cgacgctgga acttcagcag gagctacagc
    26341 aggcgctgga gagtaatccg ctgcttgagc aaatcgacac tcatgaagaa atcgacaccc
    26401 gcgaaacgca agacagtgaa acgctggaca ccgccgacgc gctcgaacaa aaagagatgc
    26461 cggaagagct gccgctcgat gccagttggg acaccattta caccgctggt acaccatccg
    26521 gcaccagcgg tgactacatt gacgatgagc tgccggtcta tcagggcgaa acgacgcaga
    26581 ccttgcagga ttacctgatg tggcaggtcg agctgacacc gttttccgac actgaccgcg
    26641 ctattgctac ctctatcgtc gatgccgttg atgacatcgg ttatctgact gtcccgctgg
    26701 aagatattct cgaaagtatg ggcgatgaag agatcgacat cgacgaggtt gaagccgtcc
    26761 ttaagcggat ccaacggttt gatccggtcg gtgtagcggc aaaagatctg cgtgactgcc
    26821 tgctaatcca actctcccaa ttcgataaaa ccacgccgtg gctggaagag gccagactga
    26881 tcattagcga tcatctcgat ctgttagcca atcacgactt ccgcacttta atgcgcgtca
    26941 cgcgtctgaa agaagatgtg ctgaaagaag ccgtcaatct gatccagtcg ctcgatccgc
    27001 gccccgggca gtcgatccag actggcgaac ctgagtatgt cattccagat gtgctggtgc
    27061 gtaagcataa cggtcactgg acggtagaac tcaacagtga cagcattccg cgtctgcaaa
    27121 tcaaccagca ctacgcctcg atgtgcaata acgcgcgcaa cgatggtgac agccagttta
    27181 tccgcagcaa tctgcaggat gccaaatggt tgatcaagag tctggaaagc cgtaacgata
    27241 cgctactgcg cgtgagtcgc tgtatcgttg aacagcagca agccttcttt gagcaaggtg
    27301 aagaatatat gaaaccgatg gtactggccg atatcgccca ggctgtcgaa atgcatgaat
    27361 cgacgatatc tcgcgtgacc acgcaaaaat acctgcatag tccacgaggc atttttgaac
    27421 tgaagtattt cttttccagt cacatcaata ccgagggcgg cggcgaagct tcctccacgg
    27481 cgattcgtgc gctggtgaag aaattaatcg cggcggaaaa cccagcgaaa ccgttgagcg
    27541 acagcaagtt aacctctttg ctgtcggaac aaggtatcat ggtggcacgc cgcactgttg
    27601 cgaagtaccg agagtcttta tccattccgc cgtcaaacca gcgtaaacag ctcgtttgac
    27661 ccaaccgata aggaagacac tatgcagctc aacattaccg gaaataacgt cgagatcacc
    27721 gaggcactgc gcgaatttgt tacagccaaa tttgccaaac ttgagcaata ttttgaccga
    27781 atcaaccagg tctatgttgt tctgaaagtg gagaaagtca cccacacctc agatgcaaca
    27841 ctgcatgtaa acggtggtga aattcatgcc agcgcggaag gtcaggacat gtacgctgcc
    27901 attgatggct taattgataa gctggcacgc cagttgacca aacataaaga taaactgaaa
    27961 caacactaat tgtccgggca attagcatgt gcatggcggc ctgttgtgcg gcacaacggg
    28021 ccatttgtac ggttaatgct ccgagcctgt tccactgttt gagtgggcag gttcttaggt
    28081 gaaattatga caaataatga tacaactcta cagcttagca gtgttcttaa cagggaatgt
    28141 acgcgaagcc gcgtccactg tcagagcaaa aaacgcgccc tggaaatcat cagcgagctg
    28201 gcggcgaaac aacttagcct gccgcctcag gtggtttttg aagctatcct gacgcgggaa
    28261 aaaatgggca gtaccggtat cggcaatggt attgccattc cgcatggcaa actggaagaa
    28321 gatactctgc gcgccgttgg cgttttcgtt cagctcgaaa cgcctatagc tttcgatgcc
    28381 atcgacaacc aaccggtgga cttacttttt gccctgctgg tgtcggcaga ccaaactaaa
    28441 acgcacctgc atactctgtc gctggtggcg aaacgtctgg cggacaaaac catctgccgc
    28501 cgtttacgcg cagcccagag cgatgaagag ctgtatcaaa tcattacgga taccgaaggt
    28561 actccggatg aagcgtagtt attcggtaat gtctctttta gacgttgtga ggagaaacag
    28621 tacatggtac tgatgatcgt cagcggacgt tcaggttcag gtaaatctgt cgccctgcgt
    28681 gcgctggaag atatgggttt ttactgcgtg gataaccttc ccgtagtgtt gttacccgat
    28741 ctggctcgaa ccctggccga tcgcgagatt tctgccgccg tcagcattga tgttcgtaat
    28801 atgccggagt caccagaaat attcgaacag gcgatgagta acctgcctga cgctttctca
    28861 ccgcaactcc tgttcctgga tgccgaccgt aataccttaa ttcgtcgtta cagtgacacg
    28921 cgccgactgc atccgctttc cagcaaaaac ctgtcgctgg aaagtgctat cgacaaagaa
    28981 agcgatttgc tggagcctct gcgttcgcga gcggatctga ttgtcgatac ctcagaaatg
    29041 tccgttcacg agctggctga aatgctgcgt acccgtctgc tgggtaaacg tgaacgcgaa
    29101 ctgaccatgg tctttgagtc tttcggcttc aaacacggta tccctatcga tgcagattac
    29161 gtctttgacg tgcgcttctt gccgaacccg cactgggatc cgaaactgcg tccaatgaca
    29221 ggtcttgata aacctgtcgc cgcgttcctc gaccgccaca cagaagtaca caattttatc
    29281 taccagacgc gaagctatct tgagctatgg ttacctatgc tggaaaccaa caaccgtagc
    29341 tacttgacgg tcgccattgg ttgtaccggc gggaagcacc gttcggtgta tattgcagag
    29401 caactggcag actacttccg ctcgcgcggt aaaaacgtcc agtcacgcca tcgtacgctg
    29461 gaaaaacgta aaccatgacc gtcaagcaaa ctgttgaaat cacaaacaag ctgggcatgc
    29521 atgcccggcc tgcaatgaag ctgtttgaat taatgcaggg ttttgacgct gaagtgctgt
    29581 tacgtaatga cgaaggcacc gaggccgaag ccaacagcgt tattgcgctg ctgatgttgg
    29641 attctgccaa aggacggcag attgaagttg aagcgaccgg cccacaggaa gaggaagcac
    29701 tggccgccgt tatcgccctc tttaactctg gttttgatga agattaatct tcatcacttt
    29761 gacatacaac tcccttcaaa ctccccccga caataagaaa atcacgtact gaaatcgttc
    29821 tcaatcaacg tcatttgtac attttgtgcg cttttcactt tcagaagaac cttaagaaaa
    29881 ccttaagagg cattgtttag gttttgttta agttaatcga ccatactgga gatcgtcaga
    29941 aaatatttcc aggagatggc atgattcgct tatctgaaca aagtcccctg ggcaccggaa
    30001 gacatcggaa atgttatgcg catccggaag atgcccaacg ctgtatcaag attgtctacc
    30061 atcgtggcga tggtggcgat aaagagatcc gccgtgagtt aaagtactac gcgcatcttg
    30121 gtcgccggtt aaaagactgg agtggaatac cgcgctatca cggtaccgtt gagacggatt
    30181 gcggaaccgg ttatgtctac gatgtgattg cagattttga cggcaaactc tccattacgc
    30241 tgaccgaatt tgctgaacaa tgccgttacg aagaagacat tgcacaactg cgccagctac
    30301 tgaaacagct gaagcgttat ttgcaggata accgtatcgt gacgatgtcg ctgaagccgc
    30361 agaatatcct ctgtcaccgc atcagtgagt cagaggttat cccggtcgtc tgcgacaaca
    30421 ttggtgaaag cacattgatt ccactggcga cctggtcgaa gtggtgttgt ctgcgtaagc
    30481 aagagcggtt atggaaacga tttatcgccc aaccggcgct ggctattgcc ctgcagaaag
    30541 atctgcaacc tcgtgagagc aaaacgctgg ccctcacttc gcgcgaagct taatccagct
    30601 ggtgttgttg cataaacggc tcaccgccta actgatacat ctgccgtaaa atccacgcct
    30661 gacggctacg cacgtagccc gatggcgagg agactttgaa acgaagtgga ttaggtaata
    30721 cagctgccag taatgcagct tccgaccggg taagtttgct cgcgggtttg tggaaataac
    30781 gttgtgccgc agcttcgacg ccaaacacac cgtcgccaaa ttcggcgata ttcaggtaaa
    30841 ccgtcaggat acgctttttg ctccagactg tttctatccc cagcgttaat ccagcttcca
    30901 gcccttttcg tacccagcta cgcccatccc ataaaaagag atttttggcc gtctgctgag
    30961 aaatcgttga agcaccgcga atacggtttt cattgcgctc gttgtgcgcc agggctttct
    31021 caatggaagc gacatcaaag ccccagtgct cagaaaattt ctgatcttct gcggcaatca
    31081 ccgccagtcc catccacggc gagatttgat ccatactgac ccagtcagaa tgtgccacgt
    31141 aacgaaaatt gccatgcagc caggcgctga cctgtcgctc gaccattacc gctgagaagg
    31201 gaacaggcgc aacactaaac aacgcgatgc ccccgcccca gaaaacggcg aggacaacca
    31261 ttaacagcaa aagaaaacgg cgaacgaaac taaacaccgt taagcggctt ttactcattc
    31321 agccagaacc agcacgcggg aaaccagctt atcaatgccg ctcgccgctt ctgcaatgtt
    31381 ctgcgccagc atatatgctg gggtggtgac aatcttattg tcttcatcaa ccacgatatc
    31441 atcgacagga cagggcacat gctccgcgcc catctcttcc agcacttctg cggtatcgat
    31501 atcagtaccg atggtcaaac gcagcgggaa atcgaaaatt ttcggcagca tcgccggggc
    31561 aatacacata aaaccaagcg gttttccggc ctgatgcatc gcttgtgcca gcgcctttaa
    31621 ttcacggtca acggtgcatt cgctaccaag agtggcaaaa ttgcttaaat tcttcgccgc
    31681 gccaaacccc cccggcacaa tcaacgcatc cagttcagcg gcatcggcct gggccagagg
    31741 acggatttca ccgcgcgtta ttcgtgccgc ttcaatcagc acgttgcgcg tttccgtcat
    31801 cgcttcgcca gttaaatggt tgataacatc aacctgctgc ttatccggtg caaagcagac
    31861 cgcctgcgca ccgctgcgtg aaatagctaa caacgtcaac accgcttcat gaatttcaga
    31921 accgtcatag acgccgcatc cgctcagaat tacgccaatt ttcttcattg tgatcatcct
    31981 ttcgcaattg actgaaacac attaattttt ttaataaaaa tggtacgcat cacacattta
    32041 actgattcat gtaacaaatc atttaagttt tgctatctta actgcgtgcg gcctgaaaaa
    32101 cagtgctgtg cccttgtaac tcatcataat aatttacggc gcagccaaga tttccctggt
    32161 gttggcgcag tattcgcgca ccccggtcta gccggggtca ttttttagtg gcttttgcca
    32221 cccacgcttt cagcacttct acgtcgtgac gccactcttc tttcatctct tcaatccatt
    32281 caccgacgtt atcttcccag gcaggaaggt caggagactg aatttgctga cccagctgtt
    32341 gcagatggcg taaccccact gaccccgccg cacctttaat tttatgtcct tcctcaacaa
    32401 tgcctttttt atcctgcgcc gtcagattcg actccagcac gctgacatag cccggcatca
    32461 tcttctcaaa caccgctaac ccgtcggtga tcagcttcgg tcctacaagt tcgagatact
    32521 gttccagcat gggaatatcg agcaatgctt ctgatttact gttctcttct gtcgtcaccg
    32581 tactctcctc atcatcctgg gtatcccaga atttcttgat catcgcggtt agcgccggaa
    32641 cagaaagcgg cttactcagc acatcatcca ttccagcatt gaggtactct tgtttgtctt
    32701 tcagcacgtt agcggttaag gccaccagcg gcggtaaatc ctctcgcgga taacgcttcg
    32761 tcagttcacg agagatatcc agcccggtca tatctggcaa ctgaatatcc agtaacacca
    32821 ggtcgtattc gcccggttta aacatctcta gcgccgcctt gccggtcatg gcgacatcaa
    32881 cgctgttacc taatttttcc agcacagaac gcgcaacaat cacgttcagt tcaatgtctt
    32941 ccaccagcag cacattcagc gccggtaaag gcatatcgtc ttcatcaaac gcatcatcga
    33001 cctcttctgc taccgacggt gcgtggatcg tcaacgtaaa ggttgaacct ttgccctgtt
    33061 cgctggtaac cgtaatatcg ccgcccatat ttttcgccag acgacgagaa acggccagac
    33121 caataccggt gccggtggca ggtttaccgc catgactgtc tttcacctgg taatacatgg
    33181 cgaaaatttt atccagctca tcctgcggaa tgccgatacc agagtcttcc acttcaaaat
    33241 gcagcatatc gccttcatcg tagcgcacgc gcacggtaac ctggccttgc tgggtgaatt
    33301 tgacggcgtt actgatgagg ttccacagga tctgccgtaa acgcgtcccg tcggtaatga
    33361 cctgatgcgg taatggcagc gtcggctcca ggttaaagcg caatcctttt tgttgcgcct
    33421 gcaatgcgga gagattttcc agatcggcaa ggaagctggt gaaatcaacc ggttgattat
    33481 caagctggac cttgcgccgt tccatcttat ccatgtcgat aatatcgtta aagatattcc
    33541 ccagcgtgac ggccgaaaca tggatggtct tgagatattt ttcctgctcg gcggtgagtt
    33601 cggtatccag cagaatgcgg ctcagaccga cgataccgtt cagcggtgta cgcaattcgt
    33661 gactgatggt ggagataaac gtcgttttgt cgcggctggc ccgttcaagc gcatcctgat
    33721 accgcttacg ctcggtaatg tctcgaccaa agcccatcaa accgtgacgt ttacccacgc
    33781 ggtcgtagta cggcacttta cggatttcaa agcaggcttt gcgcccgtcc gggtaatcca
    33841 gccactgttc ataggtcagt gacacattat gacggaacac tttttcatcg gtttcaatga
    33901 cttttgcggc ggcttccggt gagtaaacat caacaggttt caggtgaacc agttgttttt
    33961 cgctttttcc ggtcagcagc tccatcgcgc ggttacagcc ggaaaactct ttatcttcgt
    34021 tacgataaaa aaccaggtcg ggtgaagcat caaggaagga acgtaagaat gaggattgct
    34081 gctcgagctg aatttgtgtc tcttcgcgct ctttgatttc aattttcagt tggccgaagg
    34141 tttcctgcag ttctgcttcc gctttttcac gaacggcgat ttcctgattt agctgggcaa
    34201 tattatcttt taactgaacg ttgaggctca aatcgcgctc gcgcatctcc tccagttttt
    34261 gcaccagccg tgacagacgt tgtcgtgact cctccagttg ctcgacgacc accgatagaa
    34321 agtagaccgc ccacggcgta atcaacaaac caaagaagat agaacgaata acatcaatgc
    34381 tttcgacctg accatgcagc accatggtta ccgccatttg taccacaatg gcaagaacga
    34441 cgagggccag cgccagcaac attgagaagc gcaccagacc taacttcatc atcaggtcaa
    34501 cataatactg cgccagcaga cgaatttgct tcattaggga attccttcac gacaacctgc
    34561 cccaataata cccaattctg acagttacgt tataaattgt gtgagaaatg cggaattccc
    34621 ctcaacgacc tactccgttg gaggtagcca cggacgtccc agcgccgatc cctgtacgcc
    34681 atgttcattc agatagcgat ccagctcgac cattcccgtc catcgatttt cgcaccacag
    34741 aggagcaagc agcgtcgggc gacgggcgct ggcggaaatg cggtggtaga tcacttccgg
    34801 tggcgtatgg cgaatcattt ctccggcagt gagcgtgtaa tcctccagtt caataccgtt
    34861 caaacgcccc gcttcccagg ctttcgccat aatgctgcct ttcacaatat gcaacggatg
    34921 tagctttata ccgtcaacac cggtttccac tacccgctcc agcgtttgca gacattcagc
    34981 cagcccttcg cctggcagac cgacaattaa atgcgagcac accttcagcc cgcgctggcg
    35041 tgccagctgc gtcgtgcgct gataacaggc gaaatcatga ccgcgattaa tgcgatgtag
    35101 cgttttgtcg tgcacggttt gcagccccag ctccagccac acttcgtagc cctggtcttt
    35161 atattcgcaa agcagatcca gcaccgcatc cggcacgcag tccgggcgcg tgccgacaca
    35221 caaaccgaca atattggcct ggctcaccgc ctgttgatac atcgaacgca gcacctgaac
    35281 ttcggcaaag gtgctggtat acgcctggaa ataggcgagg taacgtttcg cgcgattaat
    35341 taaattcgcc tgatgcgcca gctgttcggc aatggaacga tgctgctgcg cttcgtcggc
    35401 aaacgaggca acattacaga aggtgcagcc gccgcgcccg atggtgccgt cgcgattcgg
    35461 gcaactgaaa ccgccgtgca gcgtcagctt atgcaccttt tgcccataac gacgggtgag
    35521 atcaccacca aacatattga ctaatttctg taactgcata atctgataga ccgcgccttg
    35581 aaaagaggcc aaagcctgcc atttttagct caattcggcg atgacctgga tcaatcgtcc
    35641 cggcccgctt ttatcaactg cataatcaat caaaattacc gaaatttcat gcataatcac
    35701 ataaatcact tttgcttatc ttgtgtcaga tttttttatc tccttatgga ttttaggcaa
    35761 aaacagtagc atgaaacgtc attaccaatt aatgcagtat aaaatgctgg ttttctcgtc
    35821 aattcaaggc aggataaggg ttaacacgcc tttatgacag tcaggaattg actgtttctc
    35881 taacgacttc ccttttagcc ttaaagataa aatctatttt aatttcagcc atttaataaa
    35941 gaattttgcg ctaaagcaca tttctgtacc aataagcttg ccatttgacc tgtatcagct
    36001 ttcccgataa gttggaaatc cgctggaagc tttctggatg agcagcctgc tcatcatatt
    36061 tatgcagtaa ttgagatccc ctcttcaccg tattaaccga tgcgaaaagg acaacaaggg
    36121 ggcgaatgcg aggcgcgcgt atgacacgca acccccgtcg ccacgctctt tctgtacccg
    36181 tgcgcagcgg ttcggtagtg gggttcccgc agagcctggg ggaggttcac gatatgttgt
    36241 acgataaatc ccttgagagg gataactgtg gtttcggcct gatcgcccac atagaaggcg
    36301 aacctagcca caaggtagtg cgtactgcaa tacacgcact ggcccgcatg cagcaccgtg
    36361 gcgcgattct cgccgatggt aaaaccggtg acggttgcgg cttgctgtta caaaaaccgg
    36421 atcgcttttt tcgcatcgtt gcgcaggagc gcggctggcg tttagcgaaa aactacgccg
    36481 tcgggatgct attcctgaat aaagatcctg aactcgccgc tgccgcacgc cgcatcgttg
    36541 aagaagaact gcaacgcgaa accttgtcga ttgtgggctg gcgtgatgtc cccactaacg
    36601 aaggcgtact gggtgaaatc gccctctcct ctctgccccg cattgagcaa atttttgtga
    36661 acgccccggc aggctggcgt ccgcgcgata tggagcgccg tctgtttatc gcccgccgcc
    36721 gcattgaaaa gcgtctcgaa gccgacaaag acttctacgt ctgtagccta tcgaatctgg
    36781 tgaacatcta taaaggtctg tgtatgccgg cggatctgcc gcgcttttat ctggatcttg
    36841 cggacctgcg tctggaatcg gccatttgcc tgttccacca gcgcttctcc actaacaccg
    36901 taccgcgctg gccgctggcg caaccgttcc gctatctggc gcataacggt gaaatcaaca
    36961 ccatcactgg taaccgccaa tgggcgcgtg cgcgtaccta taaattccag acgccgctta
    37021 tccctgatct gcacgacgcc gcaccgttcg tcaacgaaac cggctctgac tccagttcga
    37081 tggataacat gctggaactc ctgctggcag gcgggatgga tatcatccgc gccatgcgtc
    37141 tgttagtgcc acccgcctgg cagaacaacc cggatatgga cccggaactg cgtgccttct
    37201 ttgactttaa ctccatgcat atggagccgt gggatggccc ggcgggcatc gtgatgtccg
    37261 acggtcgttt tgccgcctgt aacctcgacc gtaacggtct gcgtccggcg cgctacgtca
    37321 tcaccaaaga taagctcatc acctgcgcct ctgaagtcgg tatctgggat taccagcctg
    37381 acgaagtggt cgaaaaaggc cgcgtcgggc caggcgaact gatggttatc gacacccgca
    37441 gtgggcatat tctgcactcg gcagaaaccg atgacgatct gaaaagccgc catccatata
    37501 aagagtggat ggagaaaaac gtccgccgac tggtaccgtt tgaagatctg cccgatgaag
    37561 aagtgggtag ccgcgaactg gacgacgaca cgcttgccag ctaccagaaa cagtttaact
    37621 acagcgcgga agagctggac tccgtaattc gcgtactggg cgaaaacggt caggaagcgg
    37681 tcggttcgat gggcgatgat accccattcg ccgtgctctc cagtcagccg cgcattattt
    37741 acgactactt ccgccagcag tttgcccagg tgactaaccc gccaatcgac ccgctgcgtg
    37801 aagcgcatgt tatgtcgctc gccaccagta tcggtcgtga aatgaacgtc ttttgcgaag
    37861 cagagggcca ggcgcaccgt ttaagcttta aatcgccgat tctgctctac tccgatttca
    37921 aacagctcac gacgatgaaa gaggagcact accgcgcaga tacgctggat atcacctttg
    37981 acgtcactaa aaccacgctc gaagcgacag tcaaagagct gtgcgacaaa gccgaaaaaa
    38041 tggtacgtag cggcaccgtg ctgctggtgc tctccgaccg gaatatcgct aaagatcgcc
    38101 tgccggttcc agccccgatg gcggttggcg cgatccagac ccgtctggtc gatcaaagcc
    38161 tgcgttgcga tgccaacatc atcgtcgaaa ccgccagcgc ccgcgatccg caccacttcg
    38221 ccgtgttgct gggcttcggc gcgacggcta tttatccata ccttgcctat gaaacgctgg
    38281 gccgcctggt agacacccat gcgattgcca aagattatcg taccgtgatg ctcaactacc
    38341 gtaacggcat caacaaaggc ttgtacaaaa tcatgtccaa aatgggcatc tccaccatcg
    38401 cctcttaccg ctgctcgaaa ctgtttgaag cggtcggtct acacgatgat gtagtgggcc
    38461 tgtgcttcca gggggcggtc agccgcattg gtggggcaag ctttgaagac ttccagcagg
    38521 atctgctgaa tctgtcgaaa cgtgcctggc tggcgcgtaa gcccatcagc cagggcggcc
    38581 tgctgaaata cgtccacggc ggcgaatacc acgcctacaa cccggacgtg gtgcgcacgc
    38641 tgcaacaagc ggtacaaagc ggcgagtaca gcgactatca ggaatacgcg aagctggtta
    38701 atgagcgtcc ggcaaccacg ctgcgcgatc tgctggcaat tacgccgggt gaaaacgcgg
    38761 tcaacattgc tgatgttgaa ccggcaagcg aactgtttaa acgcttcgat accgctgcga
    38821 tgtctatcgg cgcgttaagc ccggaagccc acgaggcgct ggcggaagcg atgaacagca
    38881 tcggcggtaa ctcaaactcc ggtgaaggcg gtgaagatcc ggcgcgctac ggcaccaaca
    38941 aagtgtcgcg catcaagcag gtggcttccg gtcgctttgg ggttactccg gcgtatctgg
    39001 tcaatgccga cgtcattcag attaaagtcg cccagggcgc gaagccgggc gaaggcggtc
    39061 agttgccggg tgataaagtc actccttaca tcgccaaact gcgctattcg gtgcccggag
    39121 tgacgctgat ctccccgccg ccgcaccacg atatctactc tatcgaggac ttagcgcagc
    39181 tcattttcga cctcaagcag gttaacccga aagcgatgat ctccgtgaag ctggtttccg
    39241 aaccgggcgt aggcaccatc gcgactggcg tggcaaaagc ttatgccgat ttaatcacca
    39301 tcgcaggcta tgacggtggc accggcgcaa gcccgctgtc gtcagtgaaa tacgcaggtt
    39361 gcccgtggga gctggggctt gttgaaaccc agcaggcgct ggttgccaat ggtctgcgtc
    39421 ataagatccg tttgcaggtc gatggcggcc tgaaaaccgg tgttgatatc atcaaagcgg
    39481 cgattctcgg cgcagaaagc ttcggcttcg gcactggccc aatggtcgcg ctcggctgta
    39541 aatatctgcg tatttgccac ctgaacaact gcgcaacggg tgtggcaact caggatgaca
    39601 aactgcgtaa gaatcactat cacggcctgc cgttcaaggt gacgaattac tttgagttta
    39661 tcgcccgtga aacccgcgag ctgatggcac agctgggggt aacgcggctg gtggatctga
    39721 ttggtcgcac cgacctgctg aaagagctgg acggtttcac cgccaaacag cagaaactgg
    39781 cgctgtcgaa gctgctggag accgccgaac cgcatccagg taaggcactc tactgcaccg
    39841 aaaacaaccc gccgtttgat aacggcctgc tgaacgcgca gttgctgcaa caggcgaaac
    39901 cgtttgtcga tgaacgccag agcaaaacct tctggttcga tatccgcaat accgatcgct
    39961 ccgtgggcgc gtcgctttca ggctatatcg cccagacgca cggcgatcag gggctggcag
    40021 ccgatcctat caaagcgtac ttcaacggca ccgcaggcca gagtttcggt gtatggaacg
    40081 cgggcggcgt ggaactgtac ctgaccgggg atgccaacga ctatgtcggt aaaggcatgg
    40141 cgggcggctt aatcgccatt cgtcctccgg ttggttccgc cttccgcagc catgaagcaa
    40201 gtattatcgg caacacctgc ctgtatggcg cgaccggtgg tcgtctgtat gccgcaggcc
    40261 gcgcgggtga acgtttcggc gtgcggaact ccggtgctat caccgtggta gaaggcattg
    40321 gcgacaacgg ctgtgaatat atgacgggcg gtattgtctg tattctgggt aaaaccggcg
    40381 ttaacttcgg tgcgggcatg accggcggct tcgcttacgt tctcgatgaa agcggtgatt
    40441 tccgcaaacg cgttaacccg gaactggtcg aggtcttaag cgttgacgat ctggcgatcc
    40501 atgaagagca tctgcgcggt cttatcaccg agcatgttca gcatactggc tctcagcgcg
    40561 gtgaagagat tctggcgaac tggtcaacct tcgccactaa atttgcgctg gttaaaccga
    40621 agtccagtga tgtaaaagca ctgctgggtc atcgtagtcg tagcgcagca gagttgcgcg
    40681 tgcaggcgca gtaaggggta gcaacaatga gtcagaatgt ttatcaattt atcgacctgc
    40741 agcgcgttga tccgccaaag aaaccgctga agatccgcaa aattgagttt gttgaaattt
    40801 acgagccgtt ttccgaaggc caggccaaag cgcaggctga ccgctgcctg tcgtgcggca
    40861 acccatactg cgagtggaaa tgtccggtac acaactacat cccgaactgg ctgaagctcg
    40921 ccaacgaggg gcgtattttt gaagcggcgg aactgtcgca ccagaccaac accctgccgg
    40981 aagtttgcgg acgagtctgc ccgcaagacc gtctgtgcga aggttcctgc actctgaacg
    41041 atgagtttgg cgcggtgacc atcggcaaca ttgagcgtta tatcaatgat aaagcgttcg
    41101 aaatgggctg gcgtccggat atgtccggcg tgaagcaaac cggtaaaaaa gtggcgatta
    41161 tcggcgcagg cccggcaggt ctggcgtgtg cggatgtcct gacgcgtaac ggcgtaaaag
    41221 ccgttgtctt cgaccgtcat ccagaaattg gcgggctgct gaccttcggt attccggcct
    41281 tcaagctgga aaaagaggta atgacgcgtc gccgtgaaat cttcaccggc atgggtattg
    41341 aattcaaact caataccgaa gtgggccgcg acgtgcagct ggacgatctg ctgagtgatt
    41401 acgatgccgt cttccttggc gtcgggactt atcagtcaat gcgcggcggg ctggaaaacg
    41461 aagacgccga tggcgtgtac gcagcgctgc cgttccttat cgccaacacc aaacagttaa
    41521 tgggctttgg tgaaacccgc gacgaaccgt tcgtcagcat ggaaggcaaa cgcgtggtgg
    41581 tccttggcgg tggcgacact gcgatggact gcgtgcgtac gtccgtgcgc cagggagcga
    41641 agcacgttac ctgtgcctat cgtcgtgatg aagagaacat gccgggttcc cgccgcgaag
    41701 tgaaaaacgc gcgggaagaa ggcgtagagt tcaaattcaa cgtccagcca ctggggattg
    41761 aagtgaacgg taacggcaaa gtcagcggcg taaaaatggt acgtaccgaa atgggcgaac
    41821 cggatgccaa aggccgtcgc cgcgcggaga tcgttgcagg ttccgaacat atcgttccgg
    41881 cagatgcagt gatcatggcg tttggtttcc gtccacacaa catggaatgg ctggcaaaac
    41941 acagcgtcga gctggattca caaggccgca tcatcgcccc ggaaggcagc gacaacgcct
    42001 tccagaccag caacccgaaa atctttgctg gcggcgatat cgtccgtggt tccgatctgg
    42061 tggtaaccgc tattgccgaa ggtcgtaagg cggcagacgg tattatgaac tggctggaag
    42121 tttaagctct gtcccagcgc cgcctcctca gaaaagagtc ggcgctgaaa ttctcctttc
    42181 tttctggaaa gcgcctcgca taatctgaag aaaaccaatg taatacgctc atctggattg
    42241 ttaccggtag atgcccggcg tatagctgat tccattattc aacgcagcgc catataaagg
    42301 gacagataat ggaatcgctc tcagagagaa cctcaacagg ctaccagcaa atccacgacg
    42361 gtattattca cctggtcgat agcgcccgga cggaaacggt acgtagcgtt aacgcgctga
    42421 tgaccgcgac atactgggaa attggccgac gaattgtcga atttgaacaa ggtggcgagg
    42481 caagggctgc gtatggtgcg cagctaatca agcgactatc aaaggattta agtctaaggt
    42541 ataagcgtgg tttctctgca aaaaacttac gccaaatgag gcttttttac ctcttttttc
    42601 aacatgttga aattcgccag acagtgtctg gcgaattaac accattggga attccgcaga
    42661 caccatctgc agaattccca tcggcaaaga tttggcagac gctgtctgcc aaattgagag
    42721 gctgtttgat atttccacag cttttgtatt gcatctccta ttttcggttt gtatattcat
    42781 gcaaataaac gaaggaaagt tgccgtgatt tacattgaac agcttgaatt aatccataaa
    42841 agtggtgatg tgttgtatcc agtaaaaata accagaaaat catcaggaaa aactgccttc
    42901 caccttgttc cattcggctt aaataagact cacgaccttc tagaggtgga agatgcttca
    42961 gaagcaattc ggctggtgat tgatgaacgt cattccattc gctgcagtac acttacagca
    43021 actattacga ataaaaaggg caagcgaatt aagcggacag gtatatacag tatcaagggt
    43081 gttaacataa aagaatacaa cgtcaggtaa tggcacagag cggtccgtca gttcaggttt
    43141 ggctctgtgc caaagatggg tcaggtcaca cctgatctaa taaatttaag ccattaaatc
    43201 agccttaacc ttcaccacca cctttttaat ctcacccggc tcgccaacaa cgcatcccgg
    43261 cttatgcggc tctcctagca taaacacggc gaacattccc ggtttcagga tgatggcttg
    43321 ctcgttttca atggtgctgc aaagctggta atcatcctca tggtggaact cttcacactg
    43381 acgcgcagtg cctgccatgc caaacagaat ccgttcttca ccgtttaata acagctggat
    43441 atcaatgtat tgctcgtgca gctccgcttt tttctcaacg ggcgattgag tgttaaacgt
    43501 catgacattc ataaaaatat tgtcgccctg taattcgtaa cgacccggcg ctttttcttg
    43561 cggtctggca gctaatgcca gcgttaacgc gtcctgtaac gcaggatgta acccagcaga
    43621 cggtaatgac cgtacttcac ccatcatcat aatttttctc cctgggccaa cagcgctgcc
    43681 ccaagtaaac ctgcatcatg gcggtagtgc gccgccagta aatcaacatg aaataccgct
    43741 ggctcctgcg ccagatacgt ttccaccaac gccagatacc cttctgccag accaacgctg
    43801 ccaccgacca ccacgcactg gcaatcagtt gtggctttaa tatcagcgat cagccttgcc
    43861 agcacatgtg cggagcggtg aatcagttgc tgcgcctgct cgtcgccctg cccggcgcgt
    43921 gtgaaaatag ttctcgcatc cgcgcccatg agttcacctt gcgctgccgc tgcaataccg
    43981 cgaccagaag caatcgcttc cacgcaacct gtgcgtccac agccgcagac tgggccgtgt
    44041 ggatcggcaa gcgtatgccc gatatgcccc gccagaccgc cagggccggt aagcagttta
    44101 ccgccgctca ctacaccgcc gccgacgcca gtggaaacgg tgataaacac catctcggtt
    44161 acatcgcctt ccagcgcctg atactctgcc catgctgcgg cctgcgcgtc gttaatggca
    44221 atggtcggca aattggtaag ttgttccagc gttttgacta acggaaagtg tagcaatcca
    44281 ccgagattat gcggattcag cgccagcaaa ctgccgtcgc gaataattcc ggttgaagcg
    44341 atggcaaccc gctgcgcatg agcttgcaac ggagagacta atgcggataa ggcatcacgc
    44401 aaggcttctg gtgtctggct ggctggcgta ggaagttcac gacgatcgcg gatctgcccg
    44461 tcagcgccaa tcagcgcggc ggcaagttta gtaccgccga tatcaatcgc cagtgtggtc
    44521 atagcaccgc ctttttcatc gctgtgttgt accactgaca aatgtgttca agacgcgtaa
    44581 tggcagaacc gaccgtcacc gcccacgcgc cgtggcgcat cgcatctgcc gcctgagcag
    44641 gcgtgttgta acgcccttcg gcaatcaccc gacatccggc ttcgctcaac gttttcacca
    44701 gcgccagatc cggctcttct ggcgtttcag gcgtggtata gccagaaagc gtagtgccaa
    44761 taatttcggc tcccagcttt tggcatgcca ggccgtcttc cggcgttgag cagtcggtca
    44821 tcgccagtaa accgtgatga tgtatacgtg ccagcagcgt ttcaacaggc accggacgcg
    44881 ggcggtcggt gccgtcaatg gcgataatgt ccgcgcccgc ctgcgccagc gcatcgacat
    44941 cttcaatata ggccgtgatg cgtaccggag aatcctccag atcgcgtttc acaattccaa
    45001 taatcggcac gctcaccacc gcacgcgtgg cttgcagatt tgccacacct tcgatgcgaa
    45061 tggcaaccgc gcccgcctgc tctgccgcta atgccatggc tgcgacgatt tcgggtttat
    45121 cgagcgggct gtcaggaatc ggctggcagg agacaatcag gccaccgtta gcagcgattt
    45181 tttgatccag ttgtgcaagt aacgacatac atcttccctt agcgaaaggc ccggtacata
    45241 gaccgggcaa caggattaac ttttggtttt gactaaatcg tttttggcgc tgccaaatgg
    45301 cacggcaccg ctaaatggtt tgccgtcgat agcgtcatga gtacgcaatg cttccgggcg
    45361 cagccaacgc tgaacgcggg aaggcatatc cagaccaatc agcaggatca ccacaaatgt
    45421 caggctgaac gagagcgatg ccagcgcagt acccagatcc agacgttgag cgatcaacgc
    45481 gccgatgatt ggggccagtg cgccacccaa tgcgccaacg ttgtaggtaa agcccaggcc
    45541 cgctgcacgc tggtcggtat cgaaataacc gccaatcagt ttcggtaaga tcccggcgat
    45601 cccttgtcca agcatttgct ggaagaacag taacagaccg agcacccaga cgtttgcgcc
    45661 gccaatcgca aataccggaa taatcagcag ctgcgaggcc agcaggctac aaacgtacgc
    45721 tttgcgggtt cccagccagt caccgaggaa gcctcctacg cagcatccca cggccgcgcc
    45781 aaagccacta aagaacagca cattggcgac agtatgcggg ttataagcca gatcggtttt
    45841 cagatacgtt ggcagcagcg cctgaatcgg ccatgagtag aggaaagcaa acaacacgac
    45901 caccatcagc attacgcccg ttggccagcg tttgcctgta ctctgcacca taaagctgat
    45961 aaagattgcg gcgcataaca gcccaagaac agcgacgatc gcggcatttt gcaggttacc
    46021 ggcgaagcag aaccacagcg cagtagccgc cgccagtgtc attacgatat tggcaatgcg
    46081 atgttcacca cggtagagaa tatccaccat tgtgcgtact ggtgctttac ctgcgtgttt
    46141 ctctttccag tcttccgctt ccgggatgtt tttacgcagc cagagagcaa agatgattgg
    46201 caaaatgccg ataaagaaca gcgcacgcca accccagacc ggaaccacca ggctatagac
    46261 ctgagcggca acgacggccc ccacagagaa gcctgaaatc aaaaaaccac tggctttgtt
    46321 acgcagatgt tttggccagc tttcaatgac ataggtggcg ctggaaccgt attcacccgc
    46381 catccccatg ccgatgacca gacgagcgat aaacatggtg atgtagcctg gcgcaaagcc
    46441 gcaggccagc gtcccggccg agaagagaac gatgctggtg accattgcca gacgacgccc
    46501 gtagcggtca cccatagcgc cgagcatcag gccgccgaac cagcgagaga taaaggctgc
    46561 ggagatcaga cttgccgcct gcaccgtcgt cagcccgaat tcaccctgta cttcggtgag
    46621 taccagggcg attaaaacga aatcaaaacc gtcaagcaga tatcccaacc aggcagcgga
    46681 aaatgcgcgc cattgtgcac ggttgagatg gcgataccac gggatattct gggttgtagt
    46741 actcattgtg agtctcccgc ggtgggcgat gcccacacgc tttggtatga aaattgtagg
    46801 gtacagatgc gtttatttcc cctcacccgg taggggcgag cgaggggaaa acaactcacc
    46861 cgcgctcttg catcaactgc tgggccagcg ccttcagttc tggcagatat ttttcatcta
    46921 ccggtccaaa cggtttgcgg cacagcggca cagaaacgac atccatataa tgcaggacag
    46981 ttttcaggcc gcggaatacg ccagttttga tcagtaaatc aatgactttg ttgcactcag
    47041 tttgcagttt ctgcgcggtc tggatatcgc cttctttcag cgccttcacg atcccctgat
    47101 atcgccagcc catgatgttg taagtactgc cgataccacc atcagcgcct gccagcaagc
    47161 cggaagcgaa gatttcgtcg taaccgttat agagcaccag atcaggatgt tcacgacgga
    47221 tctgttccat ctgatagaga tcgccagaag tctgtttcag cgcaccgacg ccaggcaacg
    47281 taaccagggt gttgatctga tccagggtca gttttacccc actcagggct ggaatgttgt
    47341 acacaaccat cggcaaacca tccgctgaat caataattgc ccgatagtga tcgcagtgtt
    47401 cttcaaagct gaaaggatag tagaacggcg tgacggcgga gacggcatcg aagccataac
    47461 gtttagccga tgccgcaagt tgttggcttt cggcggtgct gacgcaaccg acgtgggcga
    47521 tgagtttaat cttacctttc gcctcttcgg cgacgatttc cagtacctgt tcacgctcgg
    47581 aaaggctttg tacaaaggcc tcgccggtcg aaccacccac gtataaaccg tcgatgccct
    47641 gctgaatatt gaactgaacc aggcgacgca gactcgcttt atccagtgct tgttgttggt
    47701 caaaaggagt caggagtgca gccattacgc cacgtaaatt cgttgccata aatacctctg
    47761 aagtgatact tgtctgataa acgatatacc tttatacctg ttataccaga tcaattaagc
    47821 aacaccccat acagaaagct tataatgcga tctgcttcac taaagtggca ttatttcttt
    47881 ttgttggtgg tctgaccgaa agcgtgccag gtagcagaga cgctgttgag atgcgattgc
    47941 aacgcacgat cggcttcgtc aggatcatga cggcggatcg catcaacgat cgcaatatgc
    48001 tgttgatagc taacgttgtt atgctcgtgc agagcctgat cagccacggt tggacgtgcg
    48061 gcgatcagcc agtcgagcag ggcaacgtgg atcgccatga agattgggtt accagggatc
    48121 tccgccagca cgcggtggaa atcaacgtct gaacgaatga atgccgcgtt gttatccagc
    48181 gactgactgt tgatttccag tgcttttgcc agcaaatcga tttgctcatc ggtggcatgt
    48241 tcagccgcat agcgcaccag actggattca aagaacagac gtaattgttc gaaatgggca
    48301 atcccaccgg gatgagaaag gaaatctttc gccatgccgg aaaactcacc gatgatagtg
    48361 tccgcagaag gacgcgagac gcgagcgcgt tcgccgttgt ttatttgcac cagacctttg
    48421 cgttttaacg ctgccagcgc ttcgcgcaca gaaggacgcc cgacgttaaa gaacgccatc
    48481 agttcgcgtt cagacggtaa ttgttcacct tcgccaaatt cacgacggcg gatcatctgt
    48541 tccagctctt cttccaccat ttcggagagt tttttacgcg ccagcgggcg gctacgcaag
    48601 ttgcgaccaa ttgcaggtga agaatcttcg gtttgcgaat caaatgcgtt cataaggccc
    48661 attctgtaag gtcagtgtga ttaacatcat cagtgacatc ctatcacagg attgaaagta
    48721 ggggaaaatg gcaggttttt ctctttgtgc ctcatcatta ccataattaa cggaataatt
    48781 aactattgcg aaaaattaat gtaacgcaga taaacacatc ccgattgaat tattcataag
    48841 actattcaca agcattatga atattatgaa tgtgttctta caaaataatc ataagcgcat
    48901 attttttaat gaaaaatcac atcacctaca attaaaaaca cgacatccgc accataaata
    48961 gacttgcaaa aaatataaca tcattgtttt caatctgccg tttatgggat tgaccgtttt
    49021 cttttgacac ggagttcaac aatgttcggc ataattatat ctgtcatcgt attaattacg
    49081 atgggctatt tgatcctgaa aaactacaaa cctcaggtgg tgctggctgc cgcaggtgtc
    49141 ttcctgatga tgtgcggagt ctggttaggg ttcggtggtg tactcgatcc cgccaaaagc
    49201 agcggctact tgatcgtcga catttataat gaaatcctgc gcatgctgtc caaccgcatt
    49261 gccggattgg ggctgtcgat tatggcggtg ggcggttatg cccgctacat ggagcgcata
    49321 ggagccagtc gcgcgatggt gagcttgcta agccgcccgt taaaactcat tcgctcgccg
    49381 tatattattc tgtcggcaac ttacgtcatc ggccaaatca tggcgcagtt tattaccagc
    49441 gcctccggtc tgggtatgtt gctgatggtc accttatttc cgacgctggt gagtctggga
    49501 gtaagtcgcc tctctgcggt ggcggttatc gcaaccacga tgtccattga gtgggggatt
    49561 ctggaaacga actccatttt tgcagcccag gtcgcgggaa tgaaaattgc cacatacttc
    49621 ttccactacc agcttccggt cgcctcttgc gtcattatct cggtggcgat ctcccacttt
    49681 ttcgtgcaac gcgcttttga caaaaaagat aaaaatatca atcacgaaca ggcagagcaa
    49741 aaaactctcg ataatgtccc gccgctctat tacgccattt tacctgtgat gccgttaatc
    49801 ctgatgctcg gctcgctgtt cctcgcccac gtcgggctga tgaagtcaga actgcatctg
    49861 gtggtggtga tgttactaag tttgacggtg acgatgtttg ttgagttctt ccgcaagcat
    49921 aacttgcgcg aaacaatgga cgatgtgcag gcgttttttg acggcatggg tacgcagttt
    49981 gccaacgtgg taacgctggt ggtcgcgggt gaaatatttg cgaaaggctt aacgacgatt
    50041 ggcactgttg atgcggttat caggggtgcg gagcattctg gtctgggcgg tattggcgtg
    50101 atgattatta tggcgctagt cattgccatt tgtgccattg tgatgggctc tggcaatgcg
    50161 ccgtttatgt catttgccag tcttattccg aatatcgcag ccggactaca tgtaccagcg
    50221 gttgtaatga ttatgccgat gcattttgcc acgacgctag cgcgcgcggt ttcgccgatt
    50281 actgcggtgg tgaagcggac agcgatcccc atggcagtcg gtttcgtggt gaatatgatt
    50341 gccacaatca cgctatttta ttaagtcatt aaaaagacaa aacaggccgc ctgggcctgt
    50401 tttgtattac ttcacaacac gtaatgccgg tcgaccaccg cgtggtggct gcggaggttc
    50461 atcgtcagga tgagtgtcat catcgtgatc tggcttgtcg ccatcaataa ccgacataac
    50521 ggtttcgttg tctgccgatg cctcttcatc attcatgatg ctggtatctt catcgtaggc
    50581 agcttcaggc tcaaacatcg tgcctgcgcc attttcacgg gcgtagatag ccagcacggc
    50641 agccagcggc acagaaacct gacgcggaat gccaccaaag cgcgcgttaa agcgcacctc
    50701 atcattcgcc agttccagat tgccgacagc acgcggcgca atgttgagta cgatttgccc
    50761 gtcacgcgca tattccatag gaacctgcac gccagggagc gtcacatcca ccaccaggtg
    50821 cggcgtgagc tggttatcca gcaaccactc atagaatgca cgcagcagat agggacgacg
    50881 tggtgttagc tgtgacaaat ccatacagat taactccggc ccagacgcat ttcacgttct
    50941 gcttcagtta aagaagcaag gaaagagtca cgctcaaaga cgcgggtcat atagcctttc
    51001 agctctttcg cacccgggcc gctgagctcg atacccagtt gcggcagacg ccacagcagc
    51061 ggagcaagat agcaatcgac caggctgaac tcatcgctca ggaagtacgg cttctgaccg
    51121 aagaccggcg caatcgccag cagttcttcg cgcagttgct tacgtgcggc atctgcttca
    51181 gaagctgaac cgttgatgat ggtgttcatc agcgtgtacc agtctttttc gatgcgatgc
    51241 atgtacagac ggctttcacc gcgagctacc gggtaaacag gcatcagtgg cggatgcggg
    51301 aaacgctcat ccagatattc cataatgatg cgagattccc acagggtcag ctcacgatcc
    51361 accagggtcg gaacgctctg attcgggttg aggtcaatca gatcctgagg cggattgtcc
    51421 ttttccacgt gttcgatctc gaaacttaca cctttctcag ccagcacaat gcggacctga
    51481 tggctataga tgtcagtagg accggaaaac agcgtcatta ccgaacgttt gttggcagcg
    51541 acagccatga aaacctccag gtatagtcag aatttttact gctaccagcc accaggtggc
    51601 cagtcagaag ttgtgttacc caataaggaa cgactctctt tgttcgaaaa tcaaacaaaa
    51661 aatgagcaat acccgacatt tgggcagaaa attggatgat agtttaccag attttgcgac
    51721 cattgtggtg agtggatgcc ggaaatgggg aaaaagagat gcgctttagt ctgaaatagt
    51781 tgacttagac ccttattggc gatgtggttt ttgttttgcc tgtctttcag acggcagcaa
    51841 aaagcaactt cccatttttt acgctgattc agaatttagc cataaaaaaa cccgccgaag
    51901 cgggtttttt cgtaaattgt tttctgccgg agcagaagcc aattaacgtt tggagaactg
    51961 cggacgacga cgtgctttac gcagaccgac tttcttacgt tcaacctgac gagcgtcacg
    52021 agtaacgaag ccagctttac gcagttcaga acgcagggac tcgtcgtatt ccatcagagc
    52081 gcgggtgata ccgtgacgga tcgcaccagc ctgaccagag ataccaccac ctttaacggt
    52141 gatgtacagg tccagtttct caaccatgtc gaccagttcc agcggctgac gaactaccat
    52201 gcgggcagtt tcacgaccga agtactgttc cagagaacgt tggttgatta cgattttacc
    52261 gttgcccggt ttgatgaaaa cgcgagctgc ggaatttttg cggcgaccag tgccgtagta
    52321 ttgattttca gccattgcct ataatcccga ttagatgtca agaacttgcg gttgctgtgc
    52381 cgcgtggttg tgctcgttac ccgcgtaaac tttcagttta cggaacatag cacgacccag
    52441 cgggcctttt ggcaacatgc ctttaaccgc gatttcaatc acacgctcag gacggcgagc
    52501 aatcatctct tcaaagctca cttgtttgat accaccgatg tggccggtgt ggtgatagta
    52561 cactttgtca gtacgcttgt tgccggttac agcaactttg tcagcgttca gaacgatgat
    52621 gtaatcaccg gtatctacgt gcggagtgta ttccgctttg tgcttaccgc gtaggcgacg
    52681 agccagttca gtagccagac ggcccagagt tttaccggtc gcgtcaacaa cataccagtc
    52741 gcgttttacg gtttctggtt tagctgtaaa agttttcatt aaaagcttac ccaataaata
    52801 gttacacgtt ggtgaacacc caaacgtctt caattgttga ggttcacacg acaaagtccg
    52861 gcaaacctac cccttcgaat agcctatgcc agcacacaaa aagttttggg aaaaaaactt
    52921 tcttgtaacg tggggtcgca ggattataga gaagtcgggg tcaaagatcg accccttttt
    52981 gtgatttgtg acaggtttta acccgccaaa tgctcgcgct tcagatactc ttcgctttgc
    53041 atctcttgca gacgtgacag gcaacgctgg aactcaaact tcagccgatc gccctgataa
    53101 atttcataca gcggcacttc tgcactcacc actaatttga catggcgctc gtaaaactca
    53161 tccaccagcg caataaagcg ccgcgcttcg ctctccatca accgcgtcat aactggtaca
    53221 tcaaacaaca tgaccgtatg aaagagacgt gagagcgcaa tatagtcatg ctgactgcgg
    53281 gcgtcgacgc acagcgtagt aaaagagacc gccagcgtct ggttctcgac gcccattgtc
    53341 gctaatggcc gatggttgat ttctaacgtc ggtgaatttt ctcgtttccc ccccgccagc
    53401 gccaaccata gtttatccat ttgcgcccgg gtttcatcgt ggagtggcga aagccacaga
    53461 tgcgcctgag tgagtgtacg cagtcgataa tcaacaccag cgtccacgtt cattacatca
    53521 caatgctgtt taatggcatc gattgcaggc agaaaacgcg cacgttgcaa gccatttcga
    53581 taaagttcgt ccggcggaat atttgacgtc gctaccaggg taataccgcg ggcgaacaag
    53641 gctttcatca gaccgccaag tagcatggca tcggtaatat cagaaacaaa aaattcgtca
    53701 aaacagagca cgtcagtttc ggctttaaag cgatcggcaa taatttccag cggatcggtc
    53761 tgcccctgta aggcagttag ctcttcatgc acccgcagca taaaacggtg aaagtgcagg
    53821 cgctgtttcc gctctcccgg caggctttga tagaaaaggt ccatcagcca ggttttcccg
    53881 cgtcctacac cgccccacat atataagcca cgcactggcg tatgctttgt gtcttcgcgt
    53941 ttaccccaca gcttaccgac ccgcgccatt agcccactcg tcctgggggc tggtggcgtg
    54001 ctattgatga gttcctgata aataatttcc aggcggctga cggcctcttt ttgaacgtcg
    54061 tcgggttgat ggctgccttc attgagcgcc ttcaggtatt gcgatgttgg ggtaacgctt
    54121 tgcatgatct tattgttatt ccttgaataa tcggtgcgcc gttgttcacg gttgacgaaa
    54181 aaaaggccgt tctacactac gcgatatgca gtcgggattc cacttctgtg gaattaacgg
    54241 ttatagtggc ataatcagcc gcaggcatgg agcctgaagc caacacccta cggaaacaaa
    54301 agacaacggg agatgttcat gacctgggaa tatgcgctaa ttgggttagt cgtcggcatc
    54361 attattggtg ctgtggccat gcgttttggt aatcgtaaac tacgccagca acaggcgttg
    54421 cagtacgaac tggaaaagaa taaagctgaa ctggacgagt atcgcgaaga gctggttagc
    54481 cactttgccc gcagcgcgga attactggat accatggcgc acgactatcg ccagctgtat
    54541 cagcacatgg cgaaaagttc cagcagcctg ctgccggaac tgtctgctga agcaaacccg
    54601 ttccgtaatc gtctggcaga gtctgaagcc agcaacgatc aggcaccggt gcagatgcct
    54661 cgcgactatt ctgaaggcgc atccggcctg ctgcgtactg gcgcgaagcg cgactaattt
    54721 atttttcggg cgcagccatt gcgccctcct cttctctccc tccccgacta tcatttaatc
    54781 tggtgtctca ttgttagccg tctgaaaatt caataacatc aaactgtttt gaatctcttt
    54841 tcttatcatt caggtacgag agcaggaata atgaaaaaac aaccccagct gttgagtgca
    54901 ttagcgttaa gtgtcgggtt aactctctcg gcgtcatttc aggccgtcgc gtcgattcca
    54961 ggccaggttg ccgatcaggc ccctctcccc agtctggctc caatgctgga aaaagtgctt
    55021 ccggcagtgg tgagcgtacg ggtggaagga acggccagtc agggacagaa aatcccggaa
    55081 gaattcaaaa agttttttgg tgatgattta ccggagcaac ctgcacaacc cttcgaaggt
    55141 ttaggctccg gtgtcatcat caacgccagt aaaggctatg tgctgaccaa caaccatgtg
    55201 attaatcagg cacagaaaat cagtattcag ctcaatgatg ggcgcgagtt tgatgcaaaa
    55261 ctgattggta gcgatgacca gagcgatatc gccctgttac aaattcaaaa cccgagcaaa
    55321 ttaacgcaaa tcgctattgc cgactccgat aaattgcgcg tcggtgattt tgccgtagcg
    55381 gtcggtaacc catttggcct tgggcaaacc gccacctctg gcattgtttc cgcattagga
    55441 cgcagcgggt tgaatcttga aggtctggaa aactttatcc agacagatgc ttccattaac
    55501 cgcggtaact ccggcggtgc actgttaaac cttaacggtg agttaattgg catcaacact
    55561 gcaatccttg cgcctggcgg cgggagcgtc gggattggat ttgccatccc cagtaatatg
    55621 gcgcgaacac tggcgcagca gcttatcgac tttggtgaaa tcaaacgcgg tttgttaggc
    55681 atcaaaggca ccgagatgag tgccgatatc gccaaagcct tcaaccttga cgtgcagcgt
    55741 ggcgcgtttg tcagcgaagt gttgccaggt tctggctcgg cgaaagcggg cgtcaaagcg
    55801 ggcgatatta ttaccagcct caacggcaaa ccgctgaata gctttgctga gttgcgctct
    55861 cgtatcgcga ccaccgagcc gggcacaaaa gtgaaacttg gcctgctgcg taacggcaaa
    55921 ccactggaag tagaagtgac gctcgatacc agcacctctt cgtcggccag cgctgaaatg
    55981 atcgcgccag cgctggaagg tgcaacgttg agcgatggtc agctaaaaga tggcggcaaa
    56041 ggtattaaga tcgatgaagt tgtcaaagga agcccagctg ctcaggctgg cttgcaaaaa
    56101 gacgatgtga tcattggcgt caaccgcgat cgggtgaact cgattgctga aatgcgtaaa
    56161 gtgctggcgg caaaatcggc catcatcgcc ctgcaaattg tacgcggcaa tgaaagcatc
    56221 tatctgctga tgcgttaatg tcgtaaaccg ggcatcaggc ttacgtgtga tgtccggtta
    56281 actcgtggta tgctgctgcc gttccctttt ttaatgacgc ctccatcatg tttgtgaagc
    56341 tcttacgttc cgttgcgatt ggattaattg tcggcgctat tctgctggtt gccatgcctt
    56401 cgctgcgcag ccttaacccg ctttccactc cgcaatttga cagtaccgat gagacgcctg
    56461 ccagctataa tctggcggtt cgccgcgccg cgccagcggt ggttaacgtt tacaaccgtg
    56521 gtttgaacac caactctcac aaccagcttg agatccgcac cctgggatcc ggtgtaatca
    56581 tggatcaacg cggttatatc atcaccaata aacacgtcat caacgacgcc gatcagatca
    56641 tcgtcgcctt acaggatggt cgtgtatttg aagcattgct ggtgggatct gactctctaa
    56701 ccgatctggc ggtacttaaa attaatgcca ctggcggttt acctaccatt ccaatcaatg
    56761 cacgtcgcgt accgcatatt ggcgacgtgg tactggcgat cggtaacccg tacaacctcg
    56821 ggcagaccat tacccagggg attattagtg ccacgggtcg aattggtctg aacccgaccg
    56881 ggcggcaaaa cttcctgcaa accgatgctt ccattaacca cggtaactct ggcggcgcgc
    56941 tggtgaactc gctgggcgaa ctgatgggca ttaacacgct gtcgtttgat aagagtaacg
    57001 atggcgaaac gccggaaggt atcggctttg cgattccttt ccagttagca accaaaatta
    57061 tggataagct gatccgcgat ggtcgcgtga tccgcggcta cattggcatt ggcggacgtg
    57121 agatcgcacc gctgcacgca cagggcggtg gtatagatca actgcaaggg atcgtggtta
    57181 atgaagtgtc acctgacggc ccggcggcga atgcgggtat tcaggtcaac gatctgatta
    57241 tttcggtgga taacaaaccg gccatctctg ctctggagac gatggatcag gtggcggaaa
    57301 ttcgccctgg ttcggtgatc ccggttgtag tgatgcgtga tgataagcag ttaacgctgc
    57361 aggtcaccat tcaggaatat ccggcaacca attaagtcga gtgctcaaaa caaaaaaccg
    57421 gagtctgtg
//
LOCUS       DAJTZL010000006        49827 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.006, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000006 DAJTZL010000000
VERSION     DAJTZL010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 49827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49827
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.006"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..455
                     /locus_tag="OO544_000333"
     CDS             <1..455
                     /locus_tag="OO544_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HCR6766032.1"
                     /translation="VPALVITAFVARRLLRFRHMLACRRRGLIVLTDRYPQDQIPGAY
                     DGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDLVIKLNVDLEVACARKPDHKRES
                     LARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEKAITDFMTARGYH"
     gene            complement(504..2003)
                     /gene="pitB"
                     /locus_tag="OO544_000334"
     CDS             complement(504..2003)
                     /gene="pitB"
                     /locus_tag="OO544_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HCR6766033.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRYWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(2295..4154)
                     /gene="gss"
                     /locus_tag="OO544_000335"
     CDS             complement(2295..4154)
                     /gene="gss"
                     /locus_tag="OO544_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HCR6766034.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            4359..5225
                     /gene="yghU"
                     /locus_tag="OO544_000336"
     CDS             4359..5225
                     /gene="yghU"
                     /locus_tag="OO544_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HCR6766035.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGG
                     FGHFYHYAPMKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(5348..5596)
                     /gene="hybG"
                     /locus_tag="OO544_000337"
     CDS             complement(5348..5596)
                     /gene="hybG"
                     /locus_tag="OO544_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HCR6766036.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(5609..5950)
                     /gene="hypA"
                     /locus_tag="OO544_000338"
     CDS             complement(5609..5950)
                     /gene="hypA"
                     /locus_tag="OO544_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HCR6766037.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(5943..6431)
                     /gene="hybE"
                     /locus_tag="OO544_000339"
     CDS             complement(5943..6431)
                     /gene="hybE"
                     /locus_tag="OO544_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HCR6766038.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(6424..6918)
                     /locus_tag="OO544_000340"
     CDS             complement(6424..6918)
                     /locus_tag="OO544_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HCR6766039.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(6918..8621)
                     /gene="hybC"
                     /locus_tag="OO544_000341"
     CDS             complement(6918..8621)
                     /gene="hybC"
                     /locus_tag="OO544_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HCR6766040.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(8618..9796)
                     /gene="hybB"
                     /locus_tag="OO544_000342"
     CDS             complement(8618..9796)
                     /gene="hybB"
                     /locus_tag="OO544_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HCR6766041.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLKRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(9786..10772)
                     /gene="hybA"
                     /locus_tag="OO544_000343"
     CDS             complement(9786..10772)
                     /gene="hybA"
                     /locus_tag="OO544_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HCR6766042.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(10775..11893)
                     /gene="hybO"
                     /locus_tag="OO544_000344"
     CDS             complement(10775..11893)
                     /gene="hybO"
                     /locus_tag="OO544_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HCR6766043.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(12082..12369)
                     /locus_tag="OO544_000345"
     CDS             complement(12082..12369)
                     /locus_tag="OO544_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 domain-containing protein"
                     /protein_id="HCR6766044.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(12488..13375)
                     /locus_tag="OO544_000346"
     CDS             complement(12488..13375)
                     /locus_tag="OO544_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HCR6766045.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYIMYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQAAGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPAYEAALKANNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            13532..14572
                     /gene="gpr"
                     /locus_tag="OO544_000347"
     CDS             13532..14572
                     /gene="gpr"
                     /locus_tag="OO544_000347"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HCR6766046.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRIGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(14612..15106)
                     /locus_tag="OO544_000348"
     CDS             complement(14612..15106)
                     /locus_tag="OO544_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HCR6766047.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            15297..16181
                     /gene="yghA"
                     /locus_tag="OO544_000349"
     CDS             15297..16181
                     /gene="yghA"
                     /locus_tag="OO544_000349"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HCR6766048.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(16341..16766)
                     /gene="exbD"
                     /locus_tag="OO544_000350"
     CDS             complement(16341..16766)
                     /gene="exbD"
                     /locus_tag="OO544_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HCR6766049.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(16773..17507)
                     /gene="exbB"
                     /locus_tag="OO544_000351"
     CDS             complement(16773..17507)
                     /gene="exbB"
                     /locus_tag="OO544_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HCR6766050.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            17759..18946
                     /gene="metC"
                     /locus_tag="OO544_000352"
     CDS             17759..18946
                     /gene="metC"
                     /locus_tag="OO544_000352"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HCR6766051.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFDCVEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCVLFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLSNEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            19086..19745
                     /locus_tag="OO544_000353"
     CDS             19086..19745
                     /locus_tag="OO544_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR6766052.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(19785..20684)
                     /gene="yqhC"
                     /locus_tag="OO544_000354"
     CDS             complement(19785..20684)
                     /gene="yqhC"
                     /locus_tag="OO544_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HCR6766053.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVDYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            20878..22041
                     /gene="yqhD"
                     /locus_tag="OO544_000355"
     CDS             20878..22041
                     /gene="yqhD"
                     /locus_tag="OO544_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HCR6766054.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKI
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANDVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            22146..22973
                     /gene="dkgA"
                     /locus_tag="OO544_000356"
     CDS             22146..22973
                     /gene="dkgA"
                     /locus_tag="OO544_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HCR6766055.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKAPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            23173..24099
                     /locus_tag="OO544_000357"
     CDS             23173..24099
                     /locus_tag="OO544_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HCR6766056.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     ADHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPEAFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            24150..24407
                     /gene="yqhH"
                     /locus_tag="OO544_000358"
     CDS             24150..24407
                     /gene="yqhH"
                     /locus_tag="OO544_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HCR6766057.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDIKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(24450..26669)
                     /locus_tag="OO544_000359"
     CDS             complement(24450..26669)
                     /locus_tag="OO544_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HCR6766058.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSPRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCVRALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLHCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKKPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRPALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(26780..28192)
                     /gene="ftsP"
                     /locus_tag="OO544_000360"
     CDS             complement(26780..28192)
                     /gene="ftsP"
                     /locus_tag="OO544_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HCR6766059.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYDVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(28267..29004)
                     /gene="plsC"
                     /locus_tag="OO544_000361"
                     /gene_synonym="parF"
     CDS             complement(28267..29004)
                     /gene="plsC"
                     /locus_tag="OO544_000361"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HCR6766060.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(29237..31495)
                     /gene="parC"
                     /locus_tag="OO544_000362"
     CDS             complement(29237..31495)
                     /gene="parC"
                     /locus_tag="OO544_000362"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HCR6766061.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKVAVKGKSNQSVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASNGDSEE"
     gene            complement(32041..32523)
                     /locus_tag="OO544_000363"
     CDS             complement(32041..32523)
                     /locus_tag="OO544_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR6766062.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(32576..32968)
                     /gene="ygiW"
                     /locus_tag="OO544_000364"
     CDS             complement(32576..32968)
                     /gene="ygiW"
                     /locus_tag="OO544_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HCR6766063.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            33120..33779
                     /gene="qseB"
                     /locus_tag="OO544_000365"
     CDS             33120..33779
                     /gene="qseB"
                     /locus_tag="OO544_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator QseB"
                     /protein_id="HCR6766064.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            33776..35125
                     /gene="qseC"
                     /locus_tag="OO544_000366"
     CDS             33776..35125
                     /gene="qseC"
                     /locus_tag="OO544_000366"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     QseC"
                     /protein_id="HCR6766065.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHSIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVVDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHGMNVEFGNAEQGGFEAKVSW"
     gene            35235..35816
                     /gene="mdaB"
                     /locus_tag="OO544_000367"
     CDS             35235..35816
                     /gene="mdaB"
                     /locus_tag="OO544_000367"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HCR6766066.1"
                     /translation="MSNTLIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            35847..36161
                     /locus_tag="OO544_000368"
     CDS             35847..36161
                     /locus_tag="OO544_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HCR6766067.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(36209..38101)
                     /gene="parE"
                     /locus_tag="OO544_000369"
     CDS             complement(36209..38101)
                     /gene="parE"
                     /locus_tag="OO544_000369"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HCR6766068.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPNSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(38130..38711)
                     /gene="yqiA"
                     /locus_tag="OO544_000370"
     CDS             complement(38130..38711)
                     /gene="yqiA"
                     /locus_tag="OO544_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HCR6766069.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(38711..39538)
                     /gene="cpdA"
                     /locus_tag="OO544_000371"
     CDS             complement(38711..39538)
                     /gene="cpdA"
                     /locus_tag="OO544_000371"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HCR6766070.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(39563..39985)
                     /locus_tag="OO544_000372"
     CDS             complement(39563..39985)
                     /locus_tag="OO544_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HCR6766071.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(39986..40615)
                     /gene="nudF"
                     /locus_tag="OO544_000373"
     CDS             complement(39986..40615)
                     /gene="nudF"
                     /locus_tag="OO544_000373"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HCR6766072.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            40820..42301
                     /gene="tolC"
                     /locus_tag="OO544_000374"
     CDS             40820..42301
                     /gene="tolC"
                     /locus_tag="OO544_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HCR6766073.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            42449..43120
                     /locus_tag="OO544_000375"
     CDS             42449..43120
                     /locus_tag="OO544_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HCR6766074.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            43126..44286
                     /locus_tag="OO544_000376"
     CDS             43126..44286
                     /locus_tag="OO544_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HCR6766075.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKRTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFIE"
     gene            complement(44324..45112)
                     /gene="ygiD"
                     /locus_tag="OO544_000377"
     CDS             complement(44324..45112)
                     /gene="ygiD"
                     /locus_tag="OO544_000377"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HCR6766076.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPVLAQHLVELLASVPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWAMSFNEYVKANLTWQGAVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            45255..46028
                     /gene="zupT"
                     /locus_tag="OO544_000378"
     CDS             45255..46028
                     /gene="zupT"
                     /locus_tag="OO544_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HCR6766077.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(46086..46256)
                     /locus_tag="OO544_000379"
     CDS             complement(46086..46256)
                     /locus_tag="OO544_000379"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6766078.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(46517..47170)
                     /gene="ribB"
                     /locus_tag="OO544_000380"
     CDS             complement(46517..47170)
                     /gene="ribB"
                     /locus_tag="OO544_000380"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HCR6766079.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(47274..47422)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            47544..47834
                     /gene="ubiK"
                     /locus_tag="OO544_000381"
     CDS             47544..47834
                     /gene="ubiK"
                     /locus_tag="OO544_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HCR6766080.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLARLEQRMSELENRSTEIKKQPDPETLPPTL"
     gene            48117..48668
                     /locus_tag="OO544_000382"
     CDS             48117..48668
                     /locus_tag="OO544_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HCR6766081.1"
                     /translation="MSAFKKSLLVAGVAMILSNNVFADEGHGIVKFKGEVISAPCSIK
                     PGDEDLTVNLGEVADTVLKSDQKSLAEPFTIHLQDCMLSQGGTTYSKAKVTFTTANTM
                     TGQTDLLKNTKETEIGGATGVGVRILDSQSGEVTLGTPVVITFNNTNSYQELNFKARM
                     ESPGKDATPGNVYAQADYKIAYE"
     gene            48728..49735
                     /locus_tag="OO544_000383"
                     /pseudo
     CDS             48728..49735
                     /locus_tag="OO544_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480735.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
BASE COUNT        12495 a        13205 c        12357 g        11770 t
ORIGIN      
        1 cagttccggc gctggtaatt acagcgtttg tcgcccgtcg cttactgcgc tttcgtcata
       61 tgcttgcctg tcgtcgtcgc gggttaattg ttctaaccga ccgttatcct caggaccaaa
      121 ttcctggcgc ttacgatggt acggtgttcc cacctaacgt tgaaggtggt cgttttgtct
      181 catggctggc aagccaggaa cgtaaagcgt ttcactggat ggcgagccat aagcctgatc
      241 tggtcatcaa actcaatgtt gaccttgaag ttgcctgtgc acgtaaaccc gaccataaac
      301 gggaatcgct ggcgaggaag attgccataa cgccacagtt aacctttggt ggtgcacaac
      361 tggttgatat cgatgccaat cagccactgg aacaggtgtt ggttgatgca gaaaaagcga
      421 ttacggattt tatgaccgcg cgtggttatc actagtcaaa aatggaaatg cccgatcgcc
      481 aggaccgggc attttcagga aggttaaatc aactgcaatg ctatccagta cagcccacca
      541 gaaagaaaaa ttgccgccgg taaagtaaac acccacgcca tcaggatgct ggttaccgtt
      601 ttacgctgta acccaccgcc gtccaccacc atcgtccctg ctaccgcaga cgagaggacg
      661 tgtgttgtgg agacaggcat cccaatataa ctggcaaggc cgatagacac tgccgccgtc
      721 atttgtgccg ccatgccttg cgcatacgtc atgccgcgct taccaatctt ctcaccgatg
      781 gtcatcgcta cacgacgcca gccaatcatg gtgccaatgc cgagcgccag tgctaccgcc
      841 atgatgatcc acaccggagc gtactcaatg gtgcttaaca tatcgctgcg cagttttttc
      901 agcaggttct ggtcttcttt actgacgcct ggcagtttcg ccagcttcgc ggaggtatca
      961 gagatgcaca gcataatgcg gcgcagctgg ctgcgctgac tcacgcttaa cggctcgtaa
     1021 ctttccatat tgcctggcag catcgtttta acgcgcgcaa tcgcatcaaa ggtatttgcc
     1081 ggatgacagt gaaactctgt tacttgcgtg ccatcagtcg atgctgcagg caatggaggt
     1141 tccatcgcaa tcaacttctg cggcagttca ggatgctgtt gcaggtagtg ttcgaagttg
     1201 gtgacggcat cgcgggtacg ggtaatttca tagccggacg cgttcatatt gacgacgaag
     1261 ccagcagggg caatccccac cagtaccagc atcaccaggc cgatcccttt ttgtccgtcg
     1321 ttcgcgccgt gcgaaaacgc cacgcccgca gcggaaacaa tcagcgcaat acgcgtccag
     1381 aatggcggtt tacgtttgcc ttttttcttt ttgcgatctt ccggaatgcg gtgaatacgg
     1441 tcacgctttt tcgtcccgct ccagtagcgt cgcagcagga atatcaggcc tcccgcaatg
     1501 accaggccga cgatagggga aacaatcagc gaggagaaaa ttttggtcac ttcacgcagg
     1561 tttaacgcat ccatcaccga tgagccggtt aacagcgcgt tggttaaacc gatgccgata
     1621 atcgcaccaa tcaaggtgtg cgaactggag gccggtaaac cgaagaacca cgttcccagg
     1681 ttccagataa tcgccgccag cagcatggaa aagaccatcg ccaggccgtg ggttgacccc
     1741 atattcagca acaaatcggt tggcaacata tggacaatgg cataggcaac gctaagtccg
     1801 cccaataaca cgccaaaaaa gttaaaaaat gccgccatca ccacagcaag ttgtggttgc
     1861 atggcacgag tataaataac ggttgccacc gcattcgccg tgtcatgaaa accattgatt
     1921 gcttcgtaga acaacacaaa tgccagagca agcaataaca aaagccctgt gtatatatca
     1981 aggccaacaa ataaatttag catatattag attacgccat tttgaattta cgaacggacg
     2041 cattatcagt gactttaacg gcatgggcaa agtgaaatat cattttttaa cgataaaaag
     2101 agtgttattt cttgtgtatc agacaataag cattcattca aaacctttag aaataatcaa
     2161 aacacaggta ttccactggt taatatattc acatatgaaa taaataaata ttagaacata
     2221 taaatattga atattttgat taatccccgt actgattatt cttcataatc agtacggggt
     2281 tgctacaaca tgtattactt tttcaccaca attaacggtt caatatcact ctcttttttg
     2341 atgaccagtg attcatcacc gcgcaaacac gtcccaccgt agttgccgcc cacggtgaag
     2401 gtacatacct gaatgtattt accgtccact ttcggcaaac accacagttg ctgatagatg
     2461 tttttctgct cggcaaattt accgctggtt ttgtccagca cctcttcatg atggctgacc
     2521 aggtcgatat tgctaccaca gcgaccggca atcggtttca ctgcgtaacc tgttttcacc
     2581 aattcatcat taacagtgaa atcggtatcc agcaggtaac gatggtgcgg gaacagcgac
     2641 cagaggatcg gcagaatcgc tttgttgccg gggatcaccg tccacagcgg ctcaaagact
     2701 agcacttccg ggcgcagcaa tacgtcgata agacgcactt cgttttgcgg atgaccggta
     2761 cggattggca ccgcagcaaa ctcacggtcg ctaacttcac gaatctgatc aaacgcggtt
     2821 tcccacgccc aggttttcca cacgcagtta accagtcgcc cttccccatc aatcagttgc
     2881 ccggcagcat cccagcccag ttcatccaat ccacgcaaga tacgcgtttc aaagcccgcc
     2941 tggtgcagcg cctgctccat aaactgcgcg tgatagtttt cctcgatatc tttgtcctgc
     3001 atgatatgga caaacggacg tgcacgactg tgtttccagg caccagccaa ttcgttaatc
     3061 agcccttccg ccggattgaa gccgttgcct ttatagccct gctccgccca acgttcgagg
     3121 atcaagcccg cttcggtatg acaggaggcg gagtcggcgt tgtactcgta aaccttcagg
     3181 ccacgctcat ccatgcagaa atccatacga ccagtgatca tatggtgacg gcgacgctgc
     3241 caggagagac gcaaacgtgg ccagaggatt ttcgggatgt cgaacagcgc cagcaggttg
     3301 tcatctttca gcaccttgtc ggttgcgtga agatacatca ggtgcagctc gttggtggct
     3361 ttaattagct cctgctcggc actctcggta atggtgtagt aatgataagg atcctgattg
     3421 atcacctgac cgttggcctg cacataggcg ttttgcagcg gatctttttc atccagccat
     3481 ttaccgtcaa actggccttt gttttccagg cgcgctccgc tgattttcag cagctcgcct
     3541 gcaatttccg gctgcggtaa gctgtattca gtatcttccg tctggatcat ccagcccaga
     3601 atggtggtgt catcaaaagt gtctttcagg gtatagcagc cgttttcgac caccatctcc
     3661 agctcacgcg tccactgttg cccttgcggc aacggggaat gaatcacgtt ctgttccgca
     3721 atacggactt tgttgccatg caattgggta atgatggcga catggccagt gtctttaaat
     3781 tcaccgcctt tatcccagat aagaagcgca cccgcgaccg gcgcacgcgg cgagccgtta
     3841 ggaaatgcct gcaatggcag gatgttgtca ttaaccactt ctcgcaggaa gcgcagcgag
     3901 aaaatctccc acgccatacc cacgtcagta aagaccacac cgtaattcag aaagagaaaa
     3961 cggcgagcaa attcaacgca ttgccacttg tggcccatat attcgtcgtc gatatagcta
     4021 cggaatacgg cgtcatcttc gtattcctgc ggatcgagag aactgtaatc tgaagagtag
     4081 attgctaccc cacctggggc gtagcccaat aatgtcccga acggggcatc ctggctggtc
     4141 gttcctttgc tcatcacttt acctttaaca atacaaccta agcagttggc gtaattgttg
     4201 tgctctgatt acctgctcag cgacattaac cggacagagg tcagactaat catacacctc
     4261 atcgcagcgg ctggcgcaaa gggttaaaaa attcacattc tgtacagcaa gtgacctgct
     4321 acactgcttc aacactacca ctcagaaggc aactcactat gacagacaat acttatcagc
     4381 ccgcgaaagt ctggacgtgg gataaatccg ctggcggcgc gttcgccaat atcaatcgcc
     4441 cggtttctgg tccgacgcat gaaaaaacgc tgcccgttgg caaacaccca ttgcaacttt
     4501 attcgctggg aacgccgaac ggtcagaaag taacgattat gcttgaggag ctgctggcgc
     4561 tgggcgttac tggtgcagag tacgacgcct ggctgattcg tattggcgat ggcgatcaat
     4621 tctccagcgg ctttgtcgaa gtgaacccaa actcgaagat cccggcgctg cgcgatcata
     4681 cgcataatcc gccgatccgc gtgtttgaat ctggttcgat cctgctttat ctggcggaga
     4741 aatttggcta cttcctgccg caggatttgg caaagcgtac tgaaacgatg aactggctgt
     4801 tctggttaca gggcgcggca ccgttcctcg gcggtggttt tggtcacttt taccattacg
     4861 cgccgatgaa aattgagtac gccatcaacc gctttaccat ggaagccaaa cgtctgctcg
     4921 acgtgctgga taagcaactg gcgcagcata agtttgttgc gggcgatgag tacaccattg
     4981 cggatatggc gatttggccg tggtttggca atgtggtgtt aggcggcgtg tatgatgccg
     5041 cagagtttct tgatgcgggc agttataagc atgtacaacg ctgggcgaaa gaagtaggcg
     5101 aacgtccggc ggtgaaacgt gggcgtattg ttaaccgcac caacggaccg ctgaatgagc
     5161 agttgcatga gcgccatgac gccagtgatt tcgagacgaa tacggaagat aagcgtcagg
     5221 ggtaagggtt ggtgttcgtc gcagcaagcc atccaggccg gataaggcgt tcacgccgca
     5281 tccggcaatc gtgcacaatg cctgatgcga cgctgccgcg tcttatcagg cctacgaaag
     5341 ctaatcatca cgcgctggta atgtcgtaat ccatttggcg cagtgcgtct aatgtggctt
     5401 tggcttcatc ttcgtcgatg atgctcatgg caaatccgac gtgtaccagc acccactggc
     5461 ccagtagatc ggcagggtta ccttcacaaa tcagggcgat attcacgtcg cgcttgatac
     5521 cacatacttc aacctgcgca agctggtgaa tatcttcacc gacagccagc acctggcctg
     5581 gaacgccaat acacattatt aactccggtt attcaacttc aatacttttg acgatcagcg
     5641 aatcgccggt atcgacacgc aaccgctcgc cgtgacagag cggacactgc gcatcgtgct
     5701 gatgaatctc caccacctgg ctgcaatccc agcaccaagc ctgggcgggt ttatagacga
     5761 tatgtaaatc gcacccttgc gccaccgttc cgtggcagac aatttcaaaa ctaaaacgga
     5821 cggcgctctc ctcaacgcag gagagcgcgc caatttccag ccacacggcg gtgacgcgct
     5881 taacatcgtg ctgctccgcc tgccgttgga taatttcaac ggcgctctgg caaagagaca
     5941 actcatgcat tttcgccact cctgcgacca aacagcaggg cgcgacgccc tgcgtgtggt
     6001 acatccggat tcgtgactgg cagcgaaagg atcattcgtg cgcagtcatc cgtcaggcgt
     6061 tggccctctt caatcgacat gctgtgcgaa agcggcgaca tcagcgaaca ggagagatat
     6121 tgcgaaacac cgtccagttc gccaacggta aaggtcatag tgccatacgg cagttgcaga
     6181 ccaatttttt cactcacttt gcgcagcggc cagagttgat ccgggccggg gaagataact
     6241 gcactcagca tccacggggt gatcacacac cccgtccact gaccttcgaa cagcgtaaaa
     6301 tcagaaacat acaccggcat tgaaggatgc agaaaagaga gatcgtgcat cgaacgccgg
     6361 gcaatttctt caaacgctgc ctgtacttgc gccttcgggg aggtctggaa acctgctatc
     6421 tcttcagtca tgaatcgcct cccgtgggat ggcttccacg cctgattcgc gcagcgcagc
     6481 cagaacctgc tcaagcgcag gttcaatcat tgcttcaacc gtcggcgtta agccgatgtg
     6541 tggctccagc gattccggga tcacgccgac cagggtcagc tttttcggaa actcaccggt
     6601 gaagcgcagg gccgacaata cgtcggccag gccaagctga tgcggagaga ttttgttggt
     6661 aaacaacgcc ggaacttctt catcccgcag gatcatcatc gttcccggcg tgttcttttt
     6721 cgacacaatg gcatccgcaa taatcagatg atcgcgattt gccatgtcgc caagcagctc
     6781 cattcccgcc gtgccgccat cgaggatctc aacataatcc ggcagaatgt atcgttgctc
     6841 taacgcttcg acaatccgca caccgatggc ttcatcggtc agcaaaatat tgccgacccc
     6901 taagactaaa atacgcatta cagaaccttc actgaaacca cttcgttgcc gtcggcatcc
     6961 actacgtgta ccgcacaggc catgcacggg tcgaaggagt gaatggtacg caccacttcc
     7021 agcggtttat tcggatcagc aaccggtgta cccaccagcg actgctcgta aggaccgacg
     7081 tcatcattga agttacgcgg accagagttc caggttgatg gaacaactgc ctggtagtta
     7141 ctgataatac cgtctttaat aaccatccag tgagagagca taccgcgcgg cgcttcgagg
     7201 aagccaacac ctttgaactc acccgttgcc ggaatgttcg gtttcacaaa ggtggtgtga
     7261 tcgcctttgc cgatattggt gatcagtgca ctgtattgat tttgcaggat atcctgcaat
     7321 tcgcagcagt gaacggtacg accaataata cggcccagcg tggagtgcag ctgcgccact
     7381 tccagcgtgt tgccagtcag tttctgataa atcgcaacga tttcattcag tttgttctgg
     7441 gtagattcgc gacctgccgc cagtttcacc agcatattag ccagcggacc cacttctacc
     7501 gttttgccgt agaaagtcgg tgatttcacc caggaatatt tcccgtcgtc agaccaacca
     7561 tcataagccg gaatggtggt accttcccac ggtgcctgcg gcgcttcgtc tttataccag
     7621 gagtgcttcg cgctttcctg aatgcctttg atcaggtatt catcggaatg agaagtgatc
     7681 ggacgatacg aggacagatc cgcattctca atgtagccgc ccgggaacag gaagctgccg
     7741 tttttactgt cggtcgggaa ttccggcacg ctcaggtagt tcaccgcacc tttaccgcgc
     7801 gtcagccatt ccgggtagaa cgcggcaata actgcggtat caaccttata aacctgttca
     7861 acaaagtcgc tcagcttgtc gatgaaagac ttgatgtaca tcaggcgctc aaggttcagc
     7921 acgcccaaac cgtcgaggtt gatcgggttc gcgacaccac ctaccgccag gttctgaatg
     7981 tgcggcgttt taccgcccag cagcgccacg acgcggttag cgtcacgctg gcactccagc
     8041 gcctgcaggt agtgcgctac cgcaatcagg ttcacttccg gcggcagttt catcgccggg
     8101 tgaccccagt agccattggc gaaaataccc aactgaccgc tggcaaccag atctttgatc
     8161 ttgttctgaa ctttagtgaa ctcttccgga ctgttcagat gccaggtcga aacgcctttc
     8221 agcatttcgg aggctttggt tgggtcagct tgcagtgcag aagtgatgtc cacccagtcc
     8281 agcgccgaaa gctgatagaa atgaacgata tggtcatgcg tggtgtgcgc agccagaatg
     8341 atgttacgga tgtattgcgc gttaaccgga acgtcgatat tcagcgcact ttctgccgca
     8401 cgaacggaag acagcgcgtg agtggtagta catacgccac agatacgttg cacaatcatc
     8461 catgcatcgc gcggatcgcg gtttttcacg atctcttcca tgccgcgcca catggtaccg
     8521 gaagcccatg ctttcgaaac gacgccattt tcgatttcgc aatcgatgcg taaatgcccc
     8581 tcaatacggg ttaccggatc aatagtaatt ctctggctca tgctttgctc gcctcatgac
     8641 gattatgatc gttttgtttt aaaggaggaa gtatcggcag tagacgaatg agtacgatgt
     8701 aagcgcaaat ctcaatagcc acaaaaccaa tagaaatcaa cagttcttcc caggtcggga
     8761 agtaggcgta accgccgccc gggttgaatg ccaccagcga ataggtcaga cgccaggttg
     8821 cacaacctaa cagtgcgctc agtgctgaca ggaacagcat gcgggaatca ttacgcagct
     8881 tcgccacacg cagaacgacc agcgggaaga gcatcagcag gacttcaatc cagaacatca
     8941 cggagtagaa gtcaccggca aacgctaacg acagcttgtc gcgatagata agctcgccaa
     9001 agcgcagcac gatgaaaatc gccagcaaca cactgatggt gttggtcagc ttgacgaaca
     9061 gactcttttc atccggaccg ttgccacgca gacccgcctg caccagcgaa ccttcaaaga
     9121 tgacaatcga gaagcccatg atgaacgccg tcagcagcga gaacagcggc aacatttcat
     9181 agctctgcca caacggatgc accttgtagc ccgccgagat catcagtgac cccattgaag
     9241 actggtgcat ggtcggcaac agcgcgccaa gtgcaatgat gaagaacata accttgttca
     9301 gacgcttgag agaaaccttc cagcccagac gttcaaacag tgccggagca aactccagtg
     9361 ccatcacgcc gatatagatg gtcatacaga ccgccgtctc gaacagtacc gagttcacgt
     9421 tgaagtgacc cggaatgtag aagtacggca ggttccagta gcgacccacg tcgatagtga
     9481 tcgacaagcc acccagtgag taaccaaaca gactcgccaa cagcgccgga cgcaccagcg
     9541 gatggtattg cccacggtta aagacgtata ccgcccacgc cagcgcccag ccgccacagg
     9601 caaagccggt gccaatcagc aggtcaaacg cgatccacac gccccacggg aagccgccgt
     9661 tcaggtcaga gacagagccc agaccgaaca ccagacgctt cacaatcagg agcatacaga
     9721 tgacgattaa cggtccaaaa atcatgaccg gtttactgat gattttgccg cccagcggtt
     9781 gtggatcatg actcatgatc gtctcctccg tcgtgatggt cgtttttggt gttgcgacga
     9841 accagcacgg ttaagcccgc cagcacagcc agtggtagca tcatgccttt atacagggtg
     9901 tgttgaatat tttcggaacg cgcaccggta gaaagatcgt ccagtttcgg caggtcgaga
     9961 ttttcataag gcacacccgt cagtaccaga acctgagtac cgccgccctc tttctcgccg
    10021 tacagatgcg gataatattt cggcaccgta tgcaggtaag tgtcgccaga tttcagcgtc
    10081 tgacgcggat agtggtattc gctgccaggc ttcagcgcca gacgtttttt cgcctccgcc
    10141 atcagctctt cacgcgtacc gaaaatcacc gcgcccgccg ggcacacttc tacgcagcca
    10201 ggcagaccgc ctttatcgag acgttccaca cctttctggt tgcacagctc gcacttatgc
    10261 agcgcaccaa acgggttgtt gtagtcgtac ttcggcacgt tgtacggaca ggcgaccatg
    10321 cagtaacggc aaccggtgca cacgtctttg tcgtaatgga caatgccggt tttcggatct
    10381 tttttcagtg cagagaccgg gcacacagag acacagttcg gatcgacgca gtgcatacac
    10441 tgtttcttaa tgtacgcgta gccgttctcc tcctggtctt tgttgacccc tgtgccgctg
    10501 gtccacacct gaatgatgtt attggtatac ggcgacagtt tgtcgttgtt cgaccaggtc
    10561 tgttcccctt gcgggttacg ttcagggaag ttgatatcct gacacttggt gacgcaagcc
    10621 tggcagccta cgcacaaggt cgagtcgtac aacatcccca gcgatcccgg aattggcggg
    10681 cggttttcag cagccgcatg actgacagac ggcagcgcgc ccgtcagcaa tgccccgcag
    10741 gaggctgctt taataaaatt acgtctgttc acggttattc tccccgtgag tcagcgttat
    10801 ctttcttttg ctgacgaccc agttcacgca ccgccatcac gctgacaccg gcaaccagcc
    10861 caaccacacc gccgagcaaa ccaatagcgc ctgcagagac gttgccgccc tctttagcgt
    10921 taacatccgg tttctgtgaa cgcggagtct gattttcgac gttggcaagc tgatggatgc
    10981 ctttatggaa gccgatacct tcttcgttac agccatagca tgggtgacca atcgctaccg
    11041 gccacacgcc gccaacatcg cagaattgca gcgttgagca gttgccgtaa gtttctggcc
    11101 ctttacagcc gaggtggtac aggcaccagc cttcacggtg gccttcatca ccgaactctt
    11161 tggcaaaacg accagcatcg aagtgcgggc gacgttcgca gtgttcgtga atcagacggc
    11221 cataggcgaa ggtcggacgg tttttgtcat ccagtttcgg cggtttgccg taagtgatga
    11281 tgtgcgcaac ggtcgcgagg aagttgtgcg ggttcggcgg gcagcccgga atattgataa
    11341 cggttttgcc tggcagaact tcttgcaggc tgactgcgcc agttgggtta actccagctg
    11401 cggcaacacc gccccacgca gagcaggaac cgatagcgat aatcgctgct gcgccttctg
    11461 ccgctttgcg gatgtgatcc acaatcggct caccagcaac catgcaataa ataccgttat
    11521 cttttaatgg gatggaacca tccacaacca acacatactg ccctttgtac ttctcaagtg
    11581 cgttatgttt gttctcttcg acctgatgac cgaaggcggc ggaaagcact tcgtgatact
    11641 ccagagagat agtctccagc acgaggtttt ctaccgttgg atgcgttgca cgaagcagag
    11701 attccgtaca accggtgcac tcctgcgcgc caatccagat aactggcgga cgctgcgggt
    11761 tagtaaccga ttcggccatc tctgcagcgg ctttgctact taaccccatg gtggcggcta
    11821 atgctgcaca aagcttcatg aaatcacgac ggttaatgcc gtgagaatgg atgagggtgt
    11881 tatctccagt catttatagt tattccgttg cgaagacctg gcatatattt tgcctcaatc
    11941 gcaaaatcaa taatgcgatc gatgcgccat ttaccacaca tttattatgg ttatcgatat
    12001 catgatactg cggcggggcg ctaaaacgaa gggaagaagc attagtgtaa ttaattaaag
    12061 caagataata cgtatgtttg atcaattttc gaggcgataa ccggccataa aaaagcgcaa
    12121 cgtagaacag gaattattct cacgaaagaa atccattacc atctctttat ccagtccata
    12181 gcggcgcgtc agaatacccg cttcccaggc actaagctgg cgatctccgg tcttttcgta
    12241 catccggtgt gagtagccta atacaaaacc gcgtttataa tcggcacaga attttgtcac
    12301 attaaccgca ctgtcggcat gcgttgcttt taatcccgcc attaagcctt taccaaaatg
    12361 gttattcatc gttcacctca ttcgctatat atcgccatat atagcgatat tttaggcaaa
    12421 gaacgacttt tgcgcacgct attttttcat gcttgtttaa gacgtaatcc agtaagagat
    12481 aaacctatca ggagagatat ttatcgaacc atttcagtgt cctttgccag gcaagatcgg
    12541 cggcagattt gtcataacgg ggcgtggaat cattatggaa tccgtgatta acccccggat
    12601 agatatacgc ctcataaacc ttattattgg ctttcaacgc cgcctcgtaa gcaggccagc
    12661 cctcgttgat tcgggtgtcc agttccgcga agtggagtag taaaggcgct tcaatcttcg
    12721 ccacatcggc agtgggtgcc tgacgaccat aaaacggcac cgcgcaggcc agttccggat
    12781 acgcgacagc cgccgcgttc gatacgccac cgccatagca aaatccggta atacccactt
    12841 tgcctgccgc ttgtggatag cgttgcataa actcaattgc ggcaaagaaa tcattcatca
    12901 gtttggttgg atcaacctgc tgttgcagct cacgaccttt atcatcattt cccggataac
    12961 ctccaacgga acttaagccg tcaggtgcca gggcgatata ccccgccttc gccactcgcc
    13021 gtgccacatc ttcgatatac ggattcagtc cacgattctc atgcaccacc accacggctg
    13081 gcgttttgcc gctcattttt gcgggtttca ccagataacc ccgtacctcg ccgtgaccat
    13141 ttggcgaagg atacatgatg tactcagcaa caatctccgg gtcggtaaac tctacctgag
    13201 tcgccagcgc ataatttggc ttgagcaaat caaacaacgc taatgccgtc atcccgccca
    13261 ccgcatactt cgccgcaagg ttgaggaact cacgtttcga aattttcccg tgagcgtaat
    13321 agtcgtagta atccaacaac tcttgtggga aatctttggc ggttaaacgc ggcatcgttg
    13381 cactcctcag ttggtgtttt tttaagcaaa gcataagcac gtgtttttgc ccagtttttc
    13441 gtcactctgt gagccagact acgggatacg cgctggcgaa tcgctaaact agaaacattg
    13501 tttcgaaatt gaacggtgga aaggagaggt catggtctgg ttagcgaatc ccgaacgtta
    13561 cgggcagatg caataccgct attgcggaaa aagcggttta cgcctgcccg cgttatcgct
    13621 cggtttatgg cacaatttcg gtcacgttaa cgcgctggaa tcacagcgtg cgatcctgcg
    13681 taaagcgttt gatttgggca ttacgcactt tgatttagcc aacaattacg ggccgcctcc
    13741 aggaagcgca gaagagaact ttggtcgcct gctgcgggag gattttgccg cttatcgcga
    13801 tgaactgatt atctctacca aggctggcta cgatatgtgg cccggccctt acggctctgg
    13861 cggttcacgt aaatacctgc tcgccagcct cgaccaaagc ctgaagcgta tagggcttga
    13921 gtatgtcgat atcttttact ctcatcgcgt cgatgaaaat acgccgatgg aagaaaccgc
    13981 ctctgcgctg gctcatgcgg tacaaagcgg taaggcgctg tatgtcggga tctcctctta
    14041 ctcgccagag cggacgcaaa aaatggtcga gttgctgcac gagtggaaaa ttccgctttt
    14101 aattcatcaa ccttcgtaca atttactgaa ccgctgggtg gataaaagcg gcctgctgga
    14161 taccctgcaa aataacggcg tgggctgtat tgcctttact cctctggctc agggattgct
    14221 gaccggaaaa tatctcaacg gcattccgca agattcacgg atgcatcgtg aagggaataa
    14281 agttcgtggt ctgacgccga aaatgcttac cgaagccaac ctcaacagcc tgcgcttatt
    14341 gaatgaaatg gcacagcagc gtggacaatc aatggcgcaa atggcgttaa gctggttgct
    14401 gaaagatgat cgcgtgacgt cggtattgat tggtgccagc cgcgcggagc aactagagga
    14461 gaacgtgcag gcgctgaata atctgacatt tagcaccgag gagctggcgc agattgatca
    14521 gcatatcgcc gatggcgagc tgaatctgtg gcaggcgtcg tccgataaat gacctgttaa
    14581 taacgggcgc gcgaaccgcg cccgaatcag atcagtgatt atgacgagtc agtcggtcaa
    14641 gatagcccat cacaaatgca gaaagcacaa acgtcagatg gataatgacg taccacatca
    14701 gtttgttatc cgggacattt ttcgcatcca taaagacgcg cagtaagtga atggaagaaa
    14761 ttgccacaat cgacgctgct actttgtttt tcagcgacgt tgcgtccatt ttccccagcc
    14821 agttcagctt ctctttgttc tcggagatat ccagctgtga gacgaaattc tcataaccgg
    14881 aaaacatcac catcaccagc aaaccgccaa ccagcgtcat atccaccagc gacagcaaca
    14941 cgaggatcaa atctgattcc gccatcgaga agatattcgg cagtacgtga ataatctcct
    15001 ggaagaactt cagcgccagg gcaactaacg ccagcgaaag gccaaagtac acgggggcaa
    15061 gcagccagcg agaagcatac attgcatttt caagaaaacg ttccatacat tcctgtcgtg
    15121 tagttaaaac gggcaacagt atatcgcaat tgagtaaaca gcacgcaaca gcaaatcaac
    15181 atatacgtta aacctttgtc gcataattct gaggtttagg gatcttcctg gttcccttat
    15241 caacgcatcg agagcgtcta cccttaaagg acgttgaacc tgaagggaga aaaacgatgt
    15301 ctcatttaaa agacccgacc acgcagtatt acactggtga atatcccaaa cagaaacaac
    15361 cgacgccagg catccaggcg aagatgacac cggtaccgga ttgcggcgag aaaacctatg
    15421 ttggtagcgg tcgcctgaaa gatcgtaaag cactggtgac agggggcgat tccggaatag
    15481 gtcgcgctgc cgccatcgct tacgcgcgtg aaggggctga cgtggcgatc agttatcttc
    15541 ccgtggaaga agaagacgct caggatgtga aaaagatcat tgaagaatgc ggacgcaaag
    15601 ccgttctgct gccaggcgat ttaagcgatg agaaattcgc ccgttcgctg gttcacgaag
    15661 cgcacaaggc gttaggcggg ctggatatta tggcgctggt cgccgggaaa caggttgcca
    15721 ttccggatat tgcagacctc accagcgaac agtttcaaaa gacctttgcc attaacgttt
    15781 tcgcgctgtt ctggctaacc caggaagcga tccccctgct accgaaaggt gcaagtatca
    15841 tcaccacttc gtcaatccag gcataccagc caagtccgca tttactggac tatgcggcta
    15901 cgaaggcggc gattctgaac tacagccgtg gcctggcaaa acaggtcgcg gagaaaggta
    15961 ttcgggtgaa tattgtcgcg ccaggcccta tctggacggc actgcaaatt tccggcggac
    16021 aaacgcagga taagatcccg cagtttggtc agaaaacgcc gatgaagcgt gcggggcaac
    16081 cggcggaact ggcccctgta tatgtttatc tggcaagtca ggagtcgagc tacgtcaccg
    16141 cagaagtgca cggcgtgtgc ggcggcgagc atttaggtta aaaaaatgcc cggttgtgaa
    16201 aagcaaccgg gcatcattgt gaattcactc ttacctgatg tggcgaaaac gccttatccg
    16261 gcctacaaac gcacgaagat tcaacatgtt gcaatagatt ttgtaggcct gatgagcgta
    16321 gcgcatcagg caattctact ttacttcgct ttggcggttt cttcgccgac cagacctatc
    16381 ttcaggtaac ccgcctgatg cagcgtatcc attaccttca tcaacgtctc gtaatcgacg
    16441 gttttgtccg ctcggaagaa gatggtggtg tctttcttgc cttcggttaa cgcattcaac
    16501 gccgtaatca ttgtttcatc ggtgaccgga tcgttaccga taaacatcga gttgtctgcc
    16561 ttcaccgaca gataaaccgg tttttccggg cgcggctgcg gcgtgctggt agaagcaggc
    16621 aagttcacct tcacatctac cgtcgctaac ggtgccgcca ccataaagat aatcagcaga
    16681 accaacatca cgtcgataaa cggcgtcacg ttgatatcat gcatttcgcc gttatcgtcg
    16741 aggttttcgt taagatgcat tgccatcgga cattatcctg cgcgtaattt ttgtgcgaca
    16801 cgaaccggat gcgcagcggc gctggcttcc agatccaggt cacggctttg cagcaacaat
    16861 acctgcgctg caacatcacc cagcatcgct ttaaagccgc caatctggcg tgcaaatacg
    16921 ttatagataa cgaccgcagg aatcgctgcc acgaggccga ttgccgttgc taacagagct
    16981 tctgcgatac ccggcgcaac gactgccagg ttagtggtct gcgtttgcgc gataccaata
    17041 aagctgttca tgatgcccca gaccgtacca aacagaccaa cgaacggaga aatcgcgccg
    17101 atggttgcca gatagccgtt accgcggccc atttgacgac ccactgcggc gacccgacgc
    17161 tccagacgga agctggtacg ttctttaata ccttcgttat cgtcgctgcc ttctgacagt
    17221 tccagctcat tctgcgcttc attgagcaaa tgcaggctta agcttttgct accaaaatca
    17281 gcggcgatat cgttggcctg gtttaaggaa cgcgcttcag ccagcagttg ctgctcgcgc
    17341 ttaaggcgac gcttctgatt gaagaactct acgctcttac tgaagaagat tgcccaagtg
    17401 actacggagg ccaaaataag cccaatcatc acgcacttaa cgacaatatc ggcgtgctga
    17461 tacatacccc agacggaaag gtccgtctgc atcaaattat tacccactgt gtatctccag
    17521 gacgcaagtc acaaaatctg cgcataataa tatcaaaatg acgtcgaatt gatagtcgtt
    17581 ctcattacta tttgcatact gccgtacctt tgctttcttt tccttgcgtt tacgcagtaa
    17641 aaaagtcacc agcacgccat ttgcgaaaat tttctgcttt atgccaattc ttcaggatgc
    17701 gcccgcgaat attcatgcta gtttagacat ccagacgtat aaaaacagga atcccgagat
    17761 ggcggacaaa aagcttgata ctcaactggt gaatgcagga cgcagcaaaa aatacactct
    17821 cggcgcggta aatagcgtga ttcagcgcgc ttcttcgctg gtctttgact gtgtggaagc
    17881 caaaaagcac gcgacgcgca atcgcgccaa tggcgagttg ttctatggac ggcgcggaac
    17941 gttaacccat ttctccttac aacaagcgat gtgtgaactg gaaggtggcg caggctgcgt
    18001 gctatttccc tgcggggcgg cagcggttgc taattccatt cttgcttttg tcgaacaggg
    18061 cgatcatgtg ttgatgacca acaccgccta tgaaccgagt caggatttct gtagcaaaat
    18121 cctcagcaaa ctgggcgtaa cgacatcgtg gtttgatccg ctgattggtg ccgatatcgt
    18181 taagcatctg cagccaaaca ctaaaatcgt gtttctggaa tcgccaggct ccatcaccat
    18241 ggaagtccac gacgttccgg cgattgttgc cgccgtacgc agtgtggtgc cggatgccat
    18301 cattatgatc gacaacacct gggcagccgg tgtgctgttt aaggcgctgg attttggcat
    18361 cgatgtttct attcaagccg ccaccaaata tctggttggg cattcagatg cgatgattgg
    18421 cactgccgtg tgcaatgccc gttgctggga gcagctacgg gaaaacgcct atctgatggg
    18481 ccagatggtc gatgccgata ccgcctatat aaccagccgt ggcctgcgca cattaggtgt
    18541 gcgtttgcgt caacatcatg aaagcagtct gaaagtggct gaatggctgg cagaacatcc
    18601 gcaagtagcg cgagttaacc accctgctct gcctggcagt aaaggccacg aattctggaa
    18661 acgagacttt acaggcagca gcgggctatt ttcctttgtg cttaagaaaa aactcagtaa
    18721 tgaagagctg gcgaactatc tggataactt cagtttattc agcatggcct actcgtgggg
    18781 cgggtatgaa tcgttgatcc tggcgaatca accagaacat atcgccgcca ttcgcccaca
    18841 aggcgagatc gattttagcg ggaccttgat tcgcctgcat attggtctgg aagatgtcga
    18901 cgatctgatt gccgatctgg acgccggttt tgcgcgaatt gtataacatt gccacttttg
    18961 gacaattttg cagacattta attgtgaaaa gtcttaaatt gttgcgtccg ggatcaaggc
    19021 gtcccggacg aatcaggagt acaataggca gataaaggct taaacgctgt tccacaggaa
    19081 agtccatggc tgttattcaa gatatcatcg ctgcgctctg gcaacacgac tttgccgcgc
    19141 tggcggatcc tcatattgtt agcgttgttt actttgtcat gtttgccacg ctgtttttag
    19201 aaaacggcct gctgcccgcc tcatttttgc caggcgacag cttgttgata ctggcaggcg
    19261 cattgattgc ccaaggggtt atggattttc tgcctacgat tgcgattctg accgccgcag
    19321 caagtctggg ctgctggcta agttatattc aggggcgctg gttagggaat accaaaacgg
    19381 tgaaaggctg gctggcacag cttcctgcta aatatcacca gcgcgccacc tgcatgtttg
    19441 accgccacgg tctgctggcg ctgctggctg gacgttttct tgcatttgtc cgtacgctgc
    19501 tgccaaccat ggcgggaatt tccggtctgc caaaccgccg cttccagttt ttcaactggt
    19561 taagtggatt gctgtgggtc agcgtggtaa ccagttttgg ctatgcctta agtatgattc
    19621 cgttcgttaa acgccatgaa gatcaggtaa tgacgttcct gatgatcctg ccaattgcct
    19681 tgttaaccgc tggcttgtta ggcacgctgt ttgtagtgat taaaaaaaaa tactgtaacg
    19741 cctgacgatt ttccccgttc ccggctgctg taccgggaac gtatttaatt cccctgcatc
    19801 gcccgcattc ttgccgcatc ttcccccggc gtcacaccga agtaacgttt aaactcacgg
    19861 ctaaattgcg atgcgctttc atagtcgacg cgcatcgctg ctgcgctggc cttcatgccg
    19921 tcatggatga tcatcatccg cgccttatgc agacggtaat tcttcaaata ctgcaacggc
    19981 gaggtgctgg tgacagactt aaaattatga tggaacgccg atacgctcat gttggcttct
    20041 gccgccagtt gctcgacgct caggttttcg gtgtatttat tctcaatccg tttcagcacg
    20101 cggctaatca gactgaagtg agtctggcga ctgaccagcg ccagtaacgc gccgccgcaa
    20161 ggtccggtca gcacgtagta cagaatttcg cggatgatct gtttgccgag aatacgcgca
    20221 tccagtggtc gctccatcac gtcgagtaac cgctccgccg cgcataaaat ctcttctgat
    20281 aacgtggcgg agttaatccc gctggctgcc atcgacggct ggaaatgctc atcttcgcca
    20341 atgtccatca acagttcctg taactgcaaa atatcgacat tgagacgcaa tcctgccagc
    20401 ggcacctctg acgtggcata ggtttcgcac tcaaacggca acggcaccgt cagcagcagg
    20461 tattcattgg catcataacg aaacacgcgt tcattgatat aaccgatttt atgcccggaa
    20521 aagagaatta tgatgccagg ctcgtacatc accggtgtac gtgcgaaagg cgtctcgcca
    20581 tacaacaaac gcacatcggg caacagttct gacaaactat tttctttatt tttcagttta
    20641 ttaactttat ccgccagcaa gcggcaaatc tcttcacgtt tcatatcgcg taatttctta
    20701 ggaataatgc ggcaatttga ttgtgcgcaa ttttgtagca tttctccagc actctggaga
    20761 aataggcaag acattggcag aaatgagcat tgagagccag ggcgctggcg atcacaatga
    20821 aaaacatcag gcagatcgtt ctctgccctc atattggccc agcaaaggga gcaagtaatg
    20881 aacaacttta atctgcacac cccaacccgc attctgtttg gtaaaggcgc aatcgctggt
    20941 ttacgcgaac aaattcctca cgatgctcgc gtattgatta cctacggcgg cggcagcgtg
    21001 aaaaaaatcg gcgttctcga tcaagttctg gatgccctga aaggcatgga cgtgctggaa
    21061 tttggcggta ttgagccaaa cccggcttat gaaacgctga tgaacgccgt gaaactggtt
    21121 cgcgaacaga aagtgacttt cctgctggcg gttggcggcg gttctgtact ggacggcacc
    21181 aaatttatcg ccgcagcggc taactatccg gaaaatatcg atccgtggca cattctgcaa
    21241 acgggcggta aagagattaa aagcgccatc ccgatgggct gtgtgctgac gctgccagca
    21301 accggttcag aatccaacgc aggcgcggtg atctcccgta aaaccacagg cgacaagcag
    21361 gcgttccatt ctgcccatgt tcagccggta tttgccgtgc tcgatccggt ttatacctac
    21421 accctgccgc cgcgtcaggt ggctaacgac gtagtggacg cctttgtaca caccgtggaa
    21481 cagtatgtta ccaaaccggt tgatgccaaa attcaggacc gtttcgcaga aggcattttg
    21541 ctgacgctaa tcgaagatgg tccgaaagcc ctgaaagagc cagaaaacta cgatgtgcgc
    21601 gccaacgtca tgtgggcggc gactcaggcg ctgaacggtt tgattggcgc tggcgtaccg
    21661 caggactggg caacgcatat gctgggccac gaactgactg cgatgcacgg tctggatcac
    21721 gcgcaaacac tggctatcgt cctgcctgca ctgtggaatg aaaaacgcga gaccaagcgc
    21781 gctaagctgc tgcaatatgc tgaacgcgtc tggaacatca ctgaaggttc cgacgatgag
    21841 cgtattgacg ccgcgattgc cgcaacccgc aatttctttg agcaattagg cgtgccgacc
    21901 cacctctccg actacggtct ggacggcagc tccatcccgg ctttgctgaa aaaactggaa
    21961 gagcacggca tgacccaact gggcgaaaat catgacatta cgttggatgt cagccgccgt
    22021 atatacgaag ccgcccgcta agctttttac gcctcaaact ttcgttttcg ggcatttcgt
    22081 ccagacttaa gttcacaaca cctcaccgga gcctgctccg gtgagttcat ataaaggagg
    22141 aacgtatggc taatccaacc gttattaagc tacaggatgg caatgtcatg ccccagctgg
    22201 gactgggcgt ctggcaagca agtaatgagg aagtaatcac cgccattcaa aaagcgttag
    22261 aagtgggtta tcgctcgatt gataccgccg cggcctacaa gaacgaagaa ggtgtcggca
    22321 aagccctgaa aaatgcctca gtcaacagag aagaactgtt catcaccact aagctgtgga
    22381 acgacgacca caagcgcccc cgcgaagccc tgctcgacag cctgaaaaaa ctccagcttg
    22441 attatatcga cctctactta atgcactggc ccgttcccgc tatcgaccat tatgtcgaag
    22501 catggaaagg catgatcgaa ttgcaaaaag agggattaat caaaagcatc ggcgtgtgca
    22561 acttccagat ccatcacctg caacgcctga ttgatgaaac tggcgtgacg cctgtgataa
    22621 accagatcga acttcatccg ctgatgcaac aacgccagct acacgcctgg aacgcgacac
    22681 acaaaatcca gaccgaatcc tggagcccat tagcgcaagg agggaaaggc gttttcgatc
    22741 agaaagtcat tcgcgatctg gcagataaat acggcaaagc cccggcgcag attgttatcc
    22801 gctggcatct ggatagcggc ctggtggtga tcccgaaatc ggtcacacct tcacgtattg
    22861 ccgaaaactt tgatgtctgg gatttccgtc tcgacaaaga cgaactcggc gaaattgcaa
    22921 aactcgatca gggcaagcgt ctcggtcccg atcctgacca gttcggcggc taacatgcaa
    22981 attctcccgg tgacggtaat gttccgctac cggacttttc agaaatcatt tattcccctc
    23041 gcgtcccgcc cgttgttact cttccttgtt caggaatgcc aaatataagg acatcatcat
    23101 gcagagccgg aagctcttaa aagaacaact catctatatc cgggataaac gcaacggaga
    23161 ggtgaaaaac agatgaaaat aatacttctg tttttagcag ccctggcaag ttttaccgta
    23221 cacgcgcagc ctccttcaca gaccgtagaa caaacagtcc ggcagattta tcagaactat
    23281 aaatcagatg ccagtacacc ttatttcggt gaaaccggag agcgggcgat aacttcagcg
    23341 cgtattcaac aggcgcttac cctgaacgac aatcttacgc tgccgggcaa tattggctgg
    23401 ctggattatg atccggtttg tgattgtcag gattttggcg atctggtgct agaaagcgtt
    23461 gcgataaccc aaactgacgc cgatcatgcc gatgccgttg tgcgctttcg tatctttaaa
    23521 gatgataaag aaaagaccac gcagacactg aaaatggtgg cggaaaatgg tcgttgggtc
    23581 attgacgata ttgtcagcaa tcatggcagc gtcttacaag cagttaatag cgagaatgaa
    23641 aaaacgctgg ccgctttagc ttcgttgcaa aaagaacagc cggaagcctt tgttgccgaa
    23701 ctctttgaac atattgctga ttatagctgg ccgtggacgt gggtggtttc cgactcttac
    23761 cgccaggcgg ttaatgcctt ctataaaacc accttcaaga cggccaataa tcccgatgaa
    23821 gatatgcaaa tagaacggca atttatttac gacaatccga tctgttttgg cgaagagtcg
    23881 ctattttcac gcgttgatga aattcgagtc ctggagaaaa ccgccgattc cgcccgcatt
    23941 catgttcgtt ttacgctgac caatggcaac aacgaagagc aagaactggt tttacagcgg
    24001 cgcgaaggca agtgggaaat cgctgatttt atccgcccga acagcggcag cctacttaag
    24061 cagattgagg caaaaaccgc cgccagatta aagcaatgag ctgaattaaa taacaattag
    24121 ccggaacaat aaataaaagg gaacactata tgaaaacgat tttcaccgtg ggagctgttg
    24181 ttctggcaac ctgcttgctc agtggctgcg tcaatgagca aaaggtcaat caactggcga
    24241 gcaatgtaca aacattaaat gccaaaatcg cccggcttga gcaggatata aaagcactac
    24301 gcccacaaat ctatgctgcc aaatccgaag ctaacagagc caatacgcgt cttgatgctc
    24361 aggactattt tgattgcctg cgctgcttgc gtatgtacgc agaatgataa aaaaatcccc
    24421 ggcagcatgt cagttgccgg ggattttttt taacgtccaa ccgccgcttt agggcgtttc
    24481 ttcgcaccag cattcaccgg acgagattgc gtagacgacg ctttttttgc cgtagcaggc
    24541 gtctgacgct gggtcgccat cggcgtatgt ttagtcaacg ccggacgggt attgcggttc
    24601 tggcgacgag cttcacgcat ctcttcaatg gttggcgcag gcactaagca atcgcgacgg
    24661 ctgccaatca gatgcttttt gcccatcgct tccagcgccg ggcggattaa cggccagttt
    24721 gccggatcgt ggtaacgcaa caacgcttta tgcaaacgac gctgtttgtc gcccttcggt
    24781 acgaagacgt cttcgctctt ataaccaatc ttcgccagcg gttttttccc ggtgtaatac
    24841 atggtggttg agttcgccag cggcgatggg tagaagttct gtacctggtc gagacggaaa
    24901 cgatgctttt tcagccacag cgccaaattc accatatctt catcacgcgt accggggtgc
    24961 gcagagatga aatacgggat cagatactgc tctttacctg cctgtttcga gtaggtatcg
    25021 aacagctctt taaagcggtc atagctgccc atgcccggct tcatcatctt cgacaacggc
    25081 ccttcttcgg tatgttccgg agcaatcttc agataaccgc cgacgtgatg ggtcgccaac
    25141 tctttgatat agcgcggatc ttccacggcg atgtcataac gtacgcctga ggcgatgagg
    25201 atttttttaa tgcctttcag atcacgcgca cggcgataga ggttgatcgt cggttcgtgg
    25261 ttagtgtcca tgtgcggaca aatatccgga taaacgcacg acaaacggcg acaagtttgt
    25321 tcagcgcgtg gcgatttgca gtgcaacata tacatgttgg cagttggccc accgagatcg
    25381 gaaatcacgc ccgtaaaacc tggaacggtg tcgcggatcg cttcgatctc attaatgatc
    25441 gaatcttccg aacggctctg aataatgcgc ccttcgtgct cggtaataga acagaaagaa
    25501 cagccgccaa agcagccgcg cataatgttg accgaaaaac ggatcatttc gtaagccgga
    25561 atacgggcat tgccataggc cggatgtggc acgcgcttgt acggcagcgc aaaaacgctg
    25621 tccatctctt cggtagaaag cgggatagca ggcgggttga tccacacata gcggtcaccg
    25681 tgtttttgca tcaatgcgcg gacgcagcct gggttggttt cgtggtgcag aatacgcgaa
    25741 gcatgggcgt acagcacttt atcgcccttc actttctcga aagaaggcag caacacgtag
    25801 gttttttccc acggtttcgg gcgcggtggc tgcacggtta cggctttggc ttcctgcttt
    25861 ttcggtgcca ccggtttgtt atccgcgcac ggcaaatctt caccatacgg atgcgggatt
    25921 gggtcgattt ttccaggggt atcaagacgg ggggaatcca cgccgctcca gccaggcagc
    25981 gcctctttca cgataatcgc ggtattacgc acatcgcgga tttcactaat cggctcgccc
    26041 atcgccagac ggtgcgccac ctccaccagc ggacgctcac cgttaccaaa catcagcatg
    26101 tcggctttcg aatccaccag cacggaacgg cgcacggtat cggaccagta atcataatgt
    26161 gcggtacggc gcagactagc ctcaataccg ccgaggatca ccggtacatc tttccacgcc
    26221 tctttacaac gctgggtata aaccagtgtg gcgcgatccg ggcgcttacc cgcgacgtta
    26281 tccggcgtgt aggcatcgtc atgacgtaaa cggcgatcgg cggtataacg gttgatcatc
    26341 gaatccatgt tgccagcagt aacaccgaaa aacagattcg gtttacccag acgcataaag
    26401 tcgtctttgc tgctccagtc cggctgggcg atgatcccga cgcgaaagcc ctgtgcttcc
    26461 agcatacgac cgcaaatcgc catcccgaag cttgggtgat cgacatacgc gtcgccagta
    26521 accaaaatga tgtcgcagct atcccagcca agttgatcca tctcttcacg agacatcggc
    26581 aaaaacggtg ccggtccaaa acaggcggcc cagtactgcg gccaggagaa caggtcgcga
    26641 tccggttgga tcagggagat agagctcatt ttgcttccag aaatgataaa aaaataatca
    26701 aaggccgggg attataagcc ggaacgaaag agaaatcgaa aagtattcca tactcgccct
    26761 cctcgggcga gtatgaagat tacggtaccg gattgactaa cagttgccca atcgagccac
    26821 ggtccgccat ttccagcgtc tgactgttga agtagaacgg gaagtgcgcc caggaaggct
    26881 gaccgaaata aacaagcagc tccacttgtc cgtcaaccca aacggtatct ttccagcctc
    26941 tgtcttccgg gaacggcatc gcaccattca cgttacggat ctggaacatc acgccttcaa
    27001 tatggaacgc ttgcggctcg tccgcgcgta ccgtccagcg ttcccacgtt ccttgctgcg
    27061 cggtgacatc aatacggttg acgtcccaca gctgtccatt aatacccggg tcatcaccca
    27121 gactgatatc gcgactgcga atcggcgaac cggccatgat ttcagttggc agcaagcgca
    27181 tcggaagact gtctgtgacc agcggcagaa ggccggttgg gcgcagcgtt agcaccaggg
    27241 tagaaaccag aatactggat ggctcaaaga agccgcgaat acgatcaaca atgctcgccg
    27301 cttcgccaca ggtgatcgac acttcatcgc cgttgctcat atccaccaga atctcgcggc
    27361 gctcgcccgg tgccagcgaa agttgcttca ccgatacagg agcaggcagg aatccctgat
    27421 cgccagaaat cacatgtaac gggcgaccat cgctcatctg tagttgatag cgacgagagt
    27481 tcgacgcgtt cagcagtcgc aagcgcaccc agccacgcga gacttcaacg tacgggcttt
    27541 gtacaccgtt aaccagcagc gtatcaccaa caaagccgcc gcttcccggt tcgttgtatt
    27601 ctggcgtacc aaagttatcc agccgtttat cctggataat gaccggaaaa tcatccacat
    27661 cataatggtt ggggataggc agcgacttgc tgacttcatc ttccaccagc cacattccgg
    27721 caaggccgtt atagacctgc tgagccgtgc ggttgggagt attggcgtga taccacagag
    27781 tagctgcgtt ctggcgaata ggcagtacgg gtgcccagtc agcgtttggc gacatcatcc
    27841 gtgccggacc gcccatcagc gggccaggta cctgtagccc ggccaccgtc attgagacat
    27901 tttctgtcag gcggttgctg taaataagct taacatcgtc gcccttccag acgcggatag
    27961 tcggccccag gtaacgacca ttgattcccc agaccgacgc gcgtgtccct ggcgtaaatg
    28021 accagtgcgc acgttgtaca gtcataaaca gcggttgccc acggcgagat tcaagtagcg
    28081 gcggaacggg tagcggttgc tgttgcccgg ctgcgctggc cttcagggga acagcgcctg
    28141 cacaaagtgc aatccccgat gcctgaatga actgacgccg actgagtgac atatttgctc
    28201 catgtaaaac tggctaaagt aataccgtgc aggaaagtgt tccccgcaaa aatacagatt
    28261 gccgacttaa acttttccgg cggcttcgcg ttctgcgact tctttatcga gctcggcgat
    28321 tttttgttcc attatcgaac gacaatgggc agccagctca cgaacctgat ctttgccata
    28381 ctgactgacg tcaattggcg gcagcatttc gacaatcacc agaccgttgt gcagtcgatt
    28441 aagattaatc ttattcgaag ttgtagagac gcacacggga ataatcggga cgcccgccgc
    28501 aattgccgcg tgaaatgctc cagtcttgaa cggtagcagg ccgcgaccac ggctgcgggt
    28561 tccttccggg aacatccaga tggaaatacg gcgttttttg aaatgattca cgacttccgc
    28621 aatggtgccg tgagctttag tgcgattgtt tctgtcgatc aataagttgc cggttaacca
    28681 gtacaactgc ccgaagaagg ggatccacag caagctcttt ttacctaccg tcaccgtcgg
    28741 cggttgcaca atgttcgatg ctgtcaccat gtcatagttg ttctggtggt tagcgatata
    28801 gatagcattg ccgtagcttt cagcatctgc aggtttgcgg cactcaactt tcaggccgaa
    28861 cagcggcgca agacggccaa acatatgccc aaaggtggcc acatgtttcg ggttacgcgg
    28921 gctgaaaagg cagtaaatgg agccgaatac acagactaag atgctgtaaa tcacggtaat
    28981 aataagacga aagatatata gcataacacc ctctgaaggt ctgagagact ggcattgtac
    29041 gtcacctgaa atcaggttag ggcattgtta aaagccgctc tctggaaaag cgtattgtta
    29101 atcgcaacgc atgaataaac aacggtttca agggaatttt tatcgtttcc gggggttgaa
    29161 tacgctgccg gatgacgact taacgtttca tccggcgttc cttgcaagcg ggaggaaaca
    29221 ggccctcccc ggcatattac tcttcgctat caccgttgct ggcacggcga ggagagtcga
    29281 tctcaacacg atcgatacgc tgcaaaccgc gcatcaacgt accgcggcgt ccacgttcac
    29341 cagtgacttt ctgtaactct tccgggcgca gtttaatttt gcgtttccca acatgaatgg
    29401 tcagcgtgct ttgcggcggc agaacgtaca attgcgccag accatcttct ccacgcgcgg
    29461 cttctgccga tggaatgttg ataatcttgt tgcctttgcc cttcgacagc tgcggcagat
    29521 cgcttaccgg gaacatcaac atacggcctg cctgagtgat tgccagcagc atatcggaag
    29581 catcttcaat caccaccggc ggcataacat gggcattttc cggtaaggtg atcaaagcct
    29641 tacctgcacg gttacgcgcc accagatcgt taaaggtgca gacgaaaccg taacccgcat
    29701 cggaagccat cagcagtttc tgatcgtcgc tttccatcag catatggtca acggtcgccc
    29761 caggcggcaa cgttaatttg ccggtgagtg gttcgccctg accacgcgcc gacggcagcg
    29821 taatcgggtc gatggcatag ctacgaccgg tggaatcaac aaacactacc gattggttgc
    29881 tcttaccttt caccgccact ttgaagctat cacccgcttt ataattcagg cccggcgcgt
    29941 cgatatcatg gcctttagcg ctgcgtaccc agcccatctg cgacagcaca atggtgacag
    30001 gttcagacgg cagcatgtcg tgctcgctca tcgctttcgc ttcttcgcgt tcctgcaacg
    30061 gcgaacgacg atcgtcaccg taggcttgcg cgtctgcctg cagttctttc ttcagcaggt
    30121 tattcatttt acgctcggaa gccaaaatgc cctgcaactg gtcgcgctct ttttccagct
    30181 cactctgctc accgcgaatc ttcatctctt ccagtttggc aagatgacgc agtttcagtt
    30241 cgaggatcgc ttccgcctgg gtttccgtaa ggccaaaccg cgacatcagc gccggtttcg
    30301 gttcatcttc gttacgaatg atctcaatca cttcgtcgat gttgagaaac gccaccagca
    30361 aaccttccag gatatgcagg cgcttgagga ctttctccag acgatagttc agtcggcggc
    30421 gcacggtatc gcgacggaac accagccatt cggagaggat ttccagcagg tttttcaccg
    30481 ccggacgacc atccagaccg atcatattga ggttaatgcg atagctcttt tccagatcgg
    30541 tggtagcgaa gaggtggttc atcacctgat ccatatccac gcggttggaa cgcggcacaa
    30601 tcaccagacg ggtcgggttc tcgtggtcag attcatcgcg cagatcgtca accatcggca
    30661 gctttttgtt gcgcatttgc gcagcaattt gctccagtac acgcgcacct gaaacctgat
    30721 gcggtaatgc gctgataacc accgcgccat cttctttctt ccacaccgcg cgcatacgta
    30781 ctgaaccacg tccgttctcg tagattttac ggatctcggc gcgcgaagtg ataatttccg
    30841 cttcagtcgg ataatccggc ccctgcacga tatccagcag ctgatcgagc gtggttttcg
    30901 gctggtcgat taatgcgatt gccgcctgag ctacttcacg caggttatgc ggtggaatat
    30961 cggtcgccat gccgacggca ataccggtgg tgccgttaag caaaatgttt ggcagacgag
    31021 caggcagcat tttcggctcc tgcaaagtgc cgtcgaagtt tggcacccag tcagccgtcc
    31081 cctgccccag ctcgctcaat agcagctcgg aatatttcga caaccgggat tcggtgtaac
    31141 gcattgccgc gaacgatttc ggatcgtccg gcgcgcccca gttcccctga ccatcaacca
    31201 gcggataacg gtaagagaac ggctgcgcca tcaggaccat cgcttcataa caggcgctat
    31261 cgccgtgcgg atggtattta cccagtacgt caccgacggt acgggccgat tttttaaatt
    31321 tggcgctggc attcaggccc agttcagaca tcgcatacac aatgcggcgc tgaacaggtt
    31381 tcagaccatc accaataaac ggcaacgcac ggtccatgat cacgtacatg gagtagttta
    31441 agtaggcgtt ttccgtaaat tcatgtagcg caaggcgctc tgccatatcg ctcattaatt
    31501 ctgattcctc aactgattcg ccagccttca aaggggcaat attgccggag atactacatt
    31561 atctgccgcg tcgagtcaca ggagatcagc gtgaatagcg ccgccggacg tgctccgacg
    31621 gcgataaagg catcaatgtg ccttgatata caacgctttg ctgtagactt gatcggcata
    31681 gagggcagca caaggttgcg cccatgccac accactcggc atgggggtcc gctccgggcg
    31741 attcgagaag ttgaggttat gcgagtccag ggaagcctaa agcatcaaag tagtcgacta
    31801 taagtatatg gttcagccca gtttagctgt gtctccacca gtgccccgcg cctcctgcca
    31861 taagtgcggc ttgctcatgg gtacccccga gacggcttac ggcgcaccgg ataataaatt
    31921 tcgcgtatac atggtattcc ttaccggaga aattataagc ccaacaacaa tacgcctgtg
    31981 gcattgttcg ctcaaactta tcgcgagtga tttggctcac actccggtaa agattcccca
    32041 tcacgctaac ggcacataaa catccgtttc cagtcgctgc tccgtcaccc cttcggcaag
    32101 attggtgtag tgaaacagaa tcggtgcttt acgcattttc tcgccgcttg caggcagcca
    32161 gtggcgaata atgccccata tcgtgtgtga aatatcgtcc agctcgccaa cgtggcgggc
    32221 cacggcataa cgtccaccgg taagttcacc attgctcaca ccataacgat tatcgggaat
    32281 cggttcgcta acgctgccgc agatatcaaa gcgaaacgct tccggtgcgg tggtggcagg
    32341 gtcgtcccag gcgacgccaa aagtctggct ttggttaaca ggagaaagcc ccgtctcttt
    32401 acgccacatg ataaatttcg ccacactgta gttgagcaac tcagggctac agcggtgcgg
    32461 caacatcgcc acagggattg atgggaaatc gataatgttt acatccagtg tcaggtttgt
    32521 cattttgcct ccctgcgaaa ttattcccgg gaacgcgtcc cgggagcggt aacaattacg
    32581 gatttacttt gcggatctgt ttgacgtcaa tttcaacaga attccagtct ttatcgactt
    32641 caccctgaat ctcaaccgta tctttcggcg tcaccgtcac accgttccag cgtttgtggt
    32701 cgatatcaac attgatagta ccgctggcat ctttaaacac gtagagatcg tcagagatgc
    32761 gttcaacgat attgccgcgc agggttaccc aggtgtcgtc acgcagggat tttgcgcttt
    32821 ctacagtcgt tacgctgccg ttcggcccct ggaatcctcc ggcctgactt tgcgttgccg
    32881 atgggccaga aaaaccgccc tgctctgctg ccatcaccgg tgcactgcac agggccatta
    32941 ctgcgattac tgctgcgaat tttttcatgt ttattactcc ctttaatgtc tgtttccgag
    33001 catttaacaa gatagtcctt aacaacttct taagggaaaa aaataaaatt taatgctgta
    33061 cagagcgcgt tacaacacgg tttactggca gcaaatacgg ttatcgcagg gatgaaaaaa
    33121 tgcgaatttt actgatagaa gatgacatgc tgattggcga cggcatcaaa actggcctta
    33181 gtaaaatggg ttttagtgtc gactggttta cacaaggtcg tcagggaaaa gaggcgcttt
    33241 atagcgcacc ttatgatgcg gtgatcctgg atttaacctt accaggcatg gatggtcgcg
    33301 atattttgcg cgaatggcga gagaaaggtc agcgtgagcc ggtactgatc ctgaccgcgc
    33361 gtgatgcgtt agcggaacgt gtagaagggc tgcgtctggg agctgacgat tatctgtgta
    33421 aaccttttgc gttgatagaa gtcgccgcca ggctggaggc tctgatgcgc cgaaccaacg
    33481 gccaggccag caacgagctg cgccacggta acgttatgct cgaccccggc aaacgtatcg
    33541 ccacgctggc tggcgaaccc ttaacactga aaccaaaaga atttgccctg ctggaattac
    33601 tgatgcgtaa cgctggtcgg gtactgccgc gcaaactgat tgaagagaaa ctgtatacct
    33661 gggacgaaga ggtcaccagt aatgccgttg aagtgcatgt gcatcatctg cgacgcaaac
    33721 tcggtagtga ttttattcgt accgtgcatg gtattggcta cacattaggt gagaaatgaa
    33781 atttacccaa cgtcttagtc tgcgcgtcag gctgacgcta atctttttaa ttctggcctc
    33841 ggtgacctgg ctgctttcca gctttgtcgc ctggaaacaa acaacggata acgtcgatga
    33901 attgttcgac acccaactga tgctgtttgc caagcggtta agtacgctcg atctcaacga
    33961 aatcaacgcg gcggatcgca tggcacagac gccaaataaa ttaaaacacg gtcatgttga
    34021 tgacgatgcg ctgacctttg ccatctttac ccacgacggc agaatggtcc ttaatgatgg
    34081 cgataacgga gaagatattc cctatagcta tcaacgggaa ggttttgctg acgggcaact
    34141 ggtcggtgaa gacgatcctt ggcgttttgt ctggatgacc tcacctgatg gcaaatatcg
    34201 catcgttgtt ggccaggaat gggaataccg tgaagacatg gcgctggcga ttgttgccgg
    34261 gcaattgatc ccgtggctgg tcgcactgcc gattatgtta atcatcatga tggtactact
    34321 gggtcgtgaa ctcgcgccgc tgaacaaact ggcgctggca ctacgtatgc gtgaccctga
    34381 ctcggaaaaa ccactaaacg cgactggcgt acccagcgaa gtgcgaccac tggttgagtc
    34441 gctaaatcaa ctgttcgccc gcacacatgc gatgatggtt cgagaacgac gctttacctc
    34501 cgatgcagct cacgaactac gtagcccgtt aacagcgctg aaagtgcaaa ccgaagttgc
    34561 gcagctctct gacgatgatc cgcaggcgcg gaaaaaagca ctgctccaat tacattccgg
    34621 gatcgatcgc gctactcgtc tggttgatca actgctcacg ctatcgcggc tggactcact
    34681 ggataacctt caggacgtcg cggagatccc gcttgaagat ctcctgcaat cgtcggtgat
    34741 ggatatttac cacacggcgc agcaggcgaa aattgacgtg cgactgacac tcaatgccca
    34801 cagcatcaaa cgcactggac aaccgctatt gctaagtttg ttggtgcgaa accttctgga
    34861 taacgcagtg cgctacagtc cacagggcag cgtggtggac gtcacgctga atgccgataa
    34921 tttcatcgtg agggataacg gccccggtgt gacaccagag gcactggcgc gaattggcga
    34981 acgcttctat cgcccccccg gacaaaccgc taccggcagt ggacttgggc tatcgattgt
    35041 ccagcgaatc gccaaattgc atggcatgaa tgttgaattt gggaatgcgg aacaaggtgg
    35101 atttgaggcg aaggtgagct ggtaagggta agacttttgc taaattcgca aaagactttg
    35161 cacattttgc taattttacc gtaccgctct gtgacgtact atagtcgaca aacgtctcac
    35221 cttgaggtta aaaaatgagc aacaccctga ttatcaacgg cgcgaaaaaa ttcgcccact
    35281 ccaatggtca actgaacgac accctgaccg aagtcgcgga tggcacactg cgcgacctcg
    35341 ggcatgatgt tcgcatcgtt cgcgccgaca gcgactacga tgtcaaagcg gaagtacaaa
    35401 actttctctg ggctgatgtg gtgatctggc agatgccagg ctggtggatg ggcgcgccgt
    35461 ggacagtgaa aaaatacatt gatgatgtat tcaccgaagg tcacgggacg ctgtatgcca
    35521 gcgatggccg tacccgcaaa gatccgtcaa aaaaatacgg ttccggcggc ctggtacagg
    35581 gcaaaaaata tatgctttct ctgacctgga acgcaccaat ggaagccttc accgaaaaag
    35641 atcaattctt ccacggcgtt ggcgtcgacg gtgtgtatct gccgttccat aaagcaaacc
    35701 aattccttgg catggaaccg ctgccgacat ttatcgctaa tgacgtgata aaaatgcctg
    35761 atgttccccg ctatactgaa gaatatcgca agcatcttgt ggaaattttt ggttaactag
    35821 agctcaggct ttagaaggag ttaaccatgc ttaccgtaat cgcagaaatc cgtactcgtc
    35881 ctggtcaaca tcaccgtcag gcggtattgg atcagtttgc taaaatcgtt ccaaccgtac
    35941 tgaaagaaga aggttgccac ggctatgcgc caatggtgga ttgcgctgct ggcgtgagtt
    36001 tccagtctat ggcaccggat tctatcgtga tgattgagca gtgggaaagc atcgcgcacc
    36061 ttgaagcgca tctgcaaacc ccgcacatga aggcgtacag cgaagccgta aaaggcgacg
    36121 tgctggagat gaatattcgt attctgcagc cagggattta atcctgcctt gtttgcccgg
    36181 ccatcctgac cgggcaatgt tctttccttt aaacctcaat ctccgccatg tcgcctttct
    36241 cttgcaacca gttgcggcga tcttccgagc gtttcttcgc cagcagcata tccatcatcg
    36301 cgtcagtacg ctgatcgtct tcatcatcga tagtcaactg caccagacgg cgagtgttcg
    36361 gatcaagcgt ggtttcacgc aattgcatcg ggttcatttc ccccagacct ttaaaacgct
    36421 ggacgttcgg cttgcctttc ttgcgtttta attgctcaag tacgccctct ttctcttctt
    36481 ccgtcagcgc gtaataaacc tctttcccga gatcaatacg gtagagcggt ggcagtgcga
    36541 cgtaaacgtg accgtgtttc accaacgcgc ggaagtgttt tacgaacaaa gcgcagagca
    36601 gcgtggcaat gtgcagacca tcagagtccg catccgcgag gatacagatt ttgccataac
    36661 gaagctggct cagatcgtcg ctgttaggat cgataccgat cgctaccgaa atatcatgca
    36721 cttcctgcga ggccagcact tcgtcggaag aaacttccca ggtgttaagg atcttacctt
    36781 tcagcggcat gatcgcctga tattcacgat cgcgcgcctg cttggcagat ccgcctgcgg
    36841 agtcaccttc cacgaggaac agttcggtac ggttaaggtc ctgcgcggta caatcagcca
    36901 gtttgccagg cagcgccggg ccgctggtca gctttttacg caccactttt ttagccgcac
    36961 gcatacgacg ctgggcgctg gaaatcgcca tctctgccag caactccgcc gcctgaacgt
    37021 tctggttcag ccacaggata aaggcatctt tcaccacgcc agaaacgaat gccgcgcatt
    37081 gacgcgaaga gagacgctct ttcgtctgcc cggcaaactg cggatcctgc attttcaccg
    37141 acagaacata ggcgcagcga tcccagatat cttccgccga cagcttcacg ccgcgcggca
    37201 gaatgttgcg atattcacag aactcacgca tcgcgtccaa caggccctga cgcaggccat
    37261 taacatgggt accgccctgc atcgttggga tcaggttgac gtagctttcg gtcagcagtt
    37321 caccgccttc cggcagccac agtagcgccc agtccacagc ttcagtatcg ccagcgaaat
    37381 taccgataaa cggtttttct ggcagcgtcg gtaaaccgtt taccgcttcc gccaggtaat
    37441 cattcagacc gtcctgatag caccagcgct gttcggtgtt gttgatctca tctttaaagg
    37501 taatctcaac gccggggcac agtaccgctt tggctttcag cacatgcgtc aggcgtgaaa
    37561 cagaaaatcg cgggctgtca aagaaggttt catccggcca gaagtgcacg ctggtaccgg
    37621 tattgcgttt accgcaagtg ccgacaacct gtaaatcctg caccttttcg ccattttcaa
    37681 aggcgatgtt ataaacctga ccatcgcggc gcacgttaac ttcaacgcgc ttcgacaggg
    37741 cgttaaccac cgaaatcccc acgccatgca ggccgccaga gaactggtaa tttttgttag
    37801 agaatttacc gcctgcatgc agacggcaaa gaatcaattc aaccgccggg acaccctctt
    37861 ccgggtgaat atccaccggc atcccgcgcc catcgtcaat aacttctaac gactggtcag
    37921 catgtaagat tacatccacg cgttttgcgt gacccgccag tgcttcatcg acactgttat
    37981 caataacttc ttgccccaaa tggttagggc gagtggtatc ggtatacatc cccggacggc
    38041 ggcgaaccgg ctcaagcccg gtgagtacct caatggcatc agcgttataa gtttgcgtca
    38101 tggtttaagt tagcaattcg agttgatcgt cagagatggt gcagaccaag aaaatcgacg
    38161 atcgggttga aataatcttc gaagcccgtg aatgcgtggt tgccgccttc tatgacagtc
    38221 tggcggcagg aagcgtagta cgccaccgcc tggcggtaat ccagcacttc atctcccgtc
    38281 tgttgcagca gccagatcaa atccggcgct tccagcgggt caatctgcat gactttgaga
    38341 tcgtaaatat ggcgtgactc tagcacatat tgctgcccgg tgtatgggtt ctcgttctga
    38401 ccgagatagt ccgtcagcag ttcaaacggg cgcaccgccg ggtttaccac cactgcgggc
    38461 agcataaaac attgtgacaa ccaggtggcg taatatcccc ccagtgacga accgacaata
    38521 cccagcgaat cgccgccatg ttccaggaca atggattcca gcagttctgc cgcgtcggaa
    38581 ggatacggcg gcaactgcgg aatgatcatc tcaacgtcag ggtgatgttc cgccagccag
    38641 ttttttaaca agctcgcttt tgcagagcgc ggcgagctgt tgaaaccgtg taaataaaga
    38701 agcgtagaca tcagtagcct tctgaagcgg tatcaggttg gaaacgtgtg tccgccaggc
    38761 gatgcacctc ggtggtcagc gtgccatcag catgtaactc gagagtacgc cagccgggcg
    38821 cgatagtatc cagcgtaaag ttggaacagt gcggcttaaa ctgcacacag gtcgacggcg
    38881 ttgccagcag gcggcgaccg ttccagtcga gatccagctc ctgatgaata tgaccgcaca
    38941 gcaagtattt gacgtgcgga aacttcgcca gcacggtatc cagttcgccc gcgttacgca
    39001 gactgtgttg atcgagccaa ctacaacccg caggtagcgg atgatgatgc agcagcagca
    39061 acgtatggcg ttctggcgca tcggccagtt tacgttccag ccactcaagc tgaaactcgc
    39121 tcagctcacc gtgcggcacg ccaaacacct ggctatccag caacaggatt tgccattgct
    39181 caccaataaa cacccgcttc gccggggaga tacccgcatc ctgtaacgcg ctgtacatcg
    39241 cgggctggaa atcgtggttg cccggcagcc agacgcaggg cgcacgaaaa cttgcgatgc
    39301 cttcagcgaa atgctgatag gccgcagagg attgatcctg cgctaaatca cctgtcgcga
    39361 caatcaggtc gaattcgtgc tgatgtggac gaatcgcctc cagcaccgcc tggtagcttt
    39421 cccaggtgtt tacccctaac agagcttcgt gcttttgtgc aaacaggtga gtgtcggtaa
    39481 tttgtaaaat cctgactctg gcctcaccag ccagaggaag ggttaacagg ctttccaaat
    39541 ggtgtcctta ggtttcacga tgctagtaaa ccggaatcgc catcgctcca tgtgctaaac
    39601 agtatcgcaa ccagtccgct aaaaactgat taatttgatg cttttcgtcg cgttgatgca
    39661 actttttatt aggataatca taccgcgctt tgaagcgaaa aatctgctgg cttgaacaca
    39721 cttcagccac catcgcgtca tgatacagac gcaccgtcat tgacggaagg ctccagtaac
    39781 tgatcgcggg cgcagtctgt tctattgtca ccagggtagt gtatcgggtc gattccacaa
    39841 tcgtcagccg atattgtgcg tttgccacct gatagcttac agtttcgccg ggtgcgtcat
    39901 tgcgcggtaa caaacggcgc aattgtgaaa agttcatctc gcacaggcgc atcatttcag
    39961 gaaagtcagg tgtgtaacgc ttcatttatg cccactcatt ttttaacgct tgatgatgca
    40021 gctgcagcca ttgcaaagcg atgaccgacg ctgcgttgtc gattttcccc tcttctaccc
    40081 actggtatgc ctgttcccgg cttaccacat gaacgcgaat atcttcgttt tcatcagcca
    40141 gaccatgaat accgcttgcg gtcgtggcgt ccacttcgcc caccataatt gacgaacgct
    40201 cactggtgcc ccccgggctt gccaggaaac ttaacaccgg tttggtccgt ttgactatca
    40261 gtcccgcctc ttcaatcgct tcgcgacggg caacatcttc cacactttca ccctcttcta
    40321 tcatcccggc aaccatctcc agtagccagg gggtttcact ggtgtcgtat gcggcaatcc
    40381 gaatctgctc aatcagcaca acttcatcgc gcactgggtc aaagggtagc aagactgcgg
    40441 cgtgaccgcg ctcaaaaatt tcccgccgta cctcatgact catttgcccg ttgaatagac
    40501 gatgacgaaa tctataaaga tctaatgaaa aaaagccgcg ataaagtgtt tctcgtgcaa
    40561 taatttctac atcgtttttg ccaaatgtaa cgggcaggtt gtctggctta agcattgtta
    40621 atgtcctggc actaatagtg aattaaatgt gaatttcagc gacgtttgac tgccgtttga
    40681 gcagtcatgt gttaaattga ggcacattaa cgccctatgg cacgtaacgc caaccttttg
    40741 cggtagcggc ttctgctaga atccgcaata attttacagt ttgatcgcgc taaatactgc
    40801 ttcaccacaa ggaatgcaaa tgaagaaatt gctccccatt cttatcggcc tgagcctttc
    40861 tgggttcagt tcgttgagcc aggccgagaa cctgatgcaa gtttatcagc aagcacgcct
    40921 tagtaacccg gaattgcgta agtctgccgc cgatcgtgat gctgcctttg aaaaaattaa
    40981 tgaagcgcgc agtccattac tgccacagct aggtttaggt gcagattaca cctatagcaa
    41041 cggctaccgc gacgcgaacg gcatcaactc taacgcgacc agtgcgtccc tgcagttaac
    41101 tcaatccatt tttgatatgt cgaaatggcg tgcgttaacg ctgcaggaaa aagcagcagg
    41161 gattcaggac gtcacatatc agaccgatca gcaaaccttg atcctcaaca ccgcgaccgc
    41221 ttatttcaac gtgttgaatg ctattgacgt tctttcctat acacaggcac aaaaagaagc
    41281 gatctaccgt caattagatc aaaccaccca acgttttaac gtgggcctgg tagcgatcac
    41341 cgacgtgcag aacgcccgcg cgcagtacga taccgtgctg gcgaacgaag tgaccgcacg
    41401 taataacctt gataacgcgg tagagcagct gcgccagatc accggtaact actatccgga
    41461 actggcggcg ctgaatgtcg aaaactttaa aaccgacaaa ccacagccgg ttaacgcgct
    41521 gctgaaagaa gccgaaaaac gcaacctgtc gctgttacag gcacgcttga gccaggacct
    41581 ggcgcgcgag caaattcgcc aggcgcagga tggtcactta ccgacgctgg atttaacggc
    41641 ttctaccggg atttctgaca cctcttatag cggttcgaaa acccgtggtg ccgctggtac
    41701 ccagtatgac gacagcaata tgggccagaa caaagtgggc ctgagcttct cgctgccgat
    41761 ttatcagggc ggaatggtta actcgcaggt gaaacaggcc cagtacaact ttgttggtgc
    41821 cagcgagcaa ctggaaagcg cgcatcgtag cgtcgtgcaa accgtacgtt cctccttcaa
    41881 caacattaat gcatctatca gtagcattaa cgcctacaaa caagccgtag tttccgctca
    41941 aagctcatta gacgcgatgg aagcgggcta ctcggtcggt acgcgtacca ttgttgatgt
    42001 gttggatgca accaccacgc tgtacaacgc taagcaagag ctggcaaatg cgcgttataa
    42061 ctacctgatt aaccagttga atattaagtc agctctgggt acgttgaacg agcaggatct
    42121 gctggcactg aacaatgcgc tgagcaaacc ggtttccact aatccggaaa acgttgcacc
    42181 gcaaacgccg gaacagaatg ctattgctga tggttatgcg cctgatagcc cggcaccagt
    42241 cgttcagcaa acatccgcac gcactaccac cagtaacggt cataaccctt tccgtaactg
    42301 acgacgacga cggggcttcg gccccgtctg aacgtaaggc aacgtaaaga tacgggttat
    42361 ctgccgcatt cttccccctt ctcgcttcaa tttcgaccag ccatcctcta ttctgatggg
    42421 tatttaccac tggtcccgga agacaaaaat gaaacggaca aaatccatac gccacgcatc
    42481 gttccgcaaa aactggagcg cacgccatct gacaccagtc gctctcgcgg ttgccactgt
    42541 ttttatgctg gctggctgtg aaaagagtga tgaaacagtg tctctctatc aaaatgctga
    42601 cgactgttca gctgcaaacc caggcaaaag cgccgaatgt accaccgcgt acaacaatgc
    42661 gctgaaagaa gccgaacgta ctgcgccgaa atacgccacc cgtgaagact gtgttgctga
    42721 atttggtgaa ggtcagtgcc agcaggcacc agcccaggct ggcatggcac cagaaaacca
    42781 ggcgcaggcc cagcaatcca gcgggagttt ctggatgccg ctgatggccg gttacatgat
    42841 ggggcgtctg atgggcggcg gcgcgggatt tgcacagcag ccgctgttct cctcgaaaaa
    42901 cccagccagc ccggcttacg gtaaatatac cgacgcgacg ggtaaaaact atggcgcagc
    42961 ccagccaggc cgcaccatga ccgtaccgaa gacagccatg gcaccaaaac cggcgaccac
    43021 tactaccgtt acccgtggcg gttttggtga atctgttgcc aaacaaagca ctatgcagcg
    43081 tagcgcaacc ggcacctctt ctcgttcaat gggtggctga taccgatgga aagagtcagt
    43141 attaccgagc gcccggactg gcgcgaaaaa gcccacgaat acggtttcaa ttttcacacc
    43201 atgtacggcg agccgtactg gtgtgaagat gcttactaca agttgaccct cgcccaggtt
    43261 gaaaagctgg aagaagtcac cgccgaactg caccagatgt gcctgaaagt ggtggaaaaa
    43321 gtgatcgcca gcgatgagct gatgaccaaa ttccgcattc caaaacgcac ctggagtttt
    43381 gtgcgtcagt catggctgac tcaccagcca tcgctttatt cgcgtcttga tctggcatgg
    43441 gatggcactg gcgaacctaa actgctggaa aataacgccg atacaccaac gtcactatac
    43501 gaggcggcgt tctttcagtg gatctggctg gaagatcagc ttaacgccgg taacttgccg
    43561 gagggcagcg accagtttaa cagtctgcaa gaaaagctaa tcgatcgctt tgtcgagttg
    43621 cgtgaacagt atggcttcca gttgctgcat ctcacctgct gtcgcgacac ggtggaagat
    43681 cgcggaacca ttcagtattt gcaggactgc gcgacggaag ctgaaatcgc taccgagttc
    43741 ctctacattg acgatatcgg gttaggtgaa aaaggtcagt tcacggattt acaggatcag
    43801 gtaatttcca acctgttcaa gctgtatcca tgggaattta tgttgcgtga gatgttctcc
    43861 accaagctgg aggatgcagg cgtacgctgg ctggaaccgg cgtggaagag cattatctcc
    43921 aacaaagcgc ttctaccgct actatgggag atgttcccga atcacccgaa cctgctgccc
    43981 gcttattttg cggaagatga tcatccgcaa atggaaaaat atgtggttaa accgatcttc
    44041 tcccgtgaag gcgcaaacgt gtcgatcatt gagaacggca aaaccattga agcagcggaa
    44101 ggtccgtatg gcgaagaagg gatgattgtt cagcaattcc acccgttacc gaaattcggc
    44161 gacagctata tgctgattgg tagctggctg gtgaacgatc aacctgccgg aattggcatt
    44221 cgtgaagacc gtgcgttgat cacccaggat atgtctcggt tttatccaca tatttttatt
    44281 gagtaatcaa tgatgccgga tggcattcgc catccggtaa ttgttaacct atctgcaccg
    44341 acagcatact caggctgccc atttctatac catcgaccgg aatggtaatt ggctcctgcc
    44401 catcccacgc acctaagaca tacaacaacg gcagatagtg ctccggcgtt gggttcgata
    44461 acgcgccacc ttcatggtca aggtaattca ccagaggatg ctgctctacc gccccttgcc
    44521 acgtcagatt cgctttcaca tactcattaa acgacatcgc ccaaggatat ggtgaactat
    44581 caccgtgcca cttcactgta cgcaggttat gcaccacgtt gccactggcg accaacatta
    44641 ttccttcatc tcgcagcgct gccagtttgc gccccatttc gaaatgccag gcggcaggtt
    44701 tgctactgtc gatactcaac tgcaccatcg ggatatcggc gtcaggatac atcttaatca
    44761 gcacgcccca ggaaccgtgg tcaaagcccc aggcttcttt atccagcgtc accgggaccg
    44821 acgctaacag ctcaaccaga tgctgtgcca gcacaggcga acccggagca ggataatgcg
    44881 tatcgtacag cgcctgcggg aagccaccaa agtcatgaat cgtgggcggc gtctccatcg
    44941 cggtcactcc tgttccacgg gtaaaccagt gagccgaaac caccacaatc gcttgcgggc
    45001 gtggcaatgt catccccaac ttctgccagc tgcgggtata caaattatct tccagcacgt
    45061 tcatcggact accgtgacct aaaaacaatg ctggcatacg tgttgaagac atgatgatat
    45121 ccttaactaa aggtgtcatt ttgatatcct cacaatacgc ttgttcggcg gagtaagaac
    45181 ccggataaca atgatgatga tcatcagtta ttttgacgat ctgcctgaag gtgaagattt
    45241 ataaggagtt gtcgatgtca gtacctctca ttctgaccat actggcgggg gcagccacgt
    45301 ttattggcgc gtttctcggc gttctcgggc aaaaaccctc gaaccgctta ctggcgtttt
    45361 cgctaggatt tgcggcgggg atcatgttgc tcatctcatt aatggaaatg cttcctgccg
    45421 cactagcagc tgaaggaatg tcgcctgtgt tgggttatgg aatgtttatc ttcggtctgc
    45481 ttggctattt tggcctggac cgcatgttgc cacatgcgca tccgcaggat ttaatgcaaa
    45541 aatcggtgca gccgttgcca aaatcgatca agcgcacagc cattctgctc actctcggca
    45601 tcagtctgca taacttcccg gaagggattg ccacctttgt cacggcgagc agcaacctgg
    45661 agctgggatt tggcatcgca ctggccgtcg cgttgcacaa tatccctgaa ggtctggcag
    45721 tggcaggccc ggtttatgcg gcaacggggt ctaaacgcac cgcgattctg tgggcgggga
    45781 tttctggact agcagaaatt cttggtggtg tgctggcgtg gttaatcctc ggtagcatga
    45841 tttccccggt ggtcatggcg gcaatcatgg cggcggttgc aggaattatg gtggcgctct
    45901 cggttgatga attaatgccg ctcgccaaag agattgaccc taataataac cccagctatg
    45961 gcgtactatg tggaatgtca gtgatgggat tcagtttagt gctgctacaa acggcgggaa
    46021 ttggttaaaa acaacatcag aagttgtcca gcacatggac aacttctgcc agcttcacga
    46081 aagccttatt ctaccttttc ggctgtctct tctctcgtac tgtttaagta tttgttccgc
    46141 gcttcaagca gtgcgtctct gtcctggcga aacgcccgac aataacgttt caaatgcagg
    46201 aaataaccca ccacaaccag agcaatcaat acaatccagt accaggcgat aaacatcctt
    46261 ttacccttaa tatttaacaa tactaaacat ttagcgcata aatttcacat atcctttttc
    46321 ggatatattc atatggtcgc ataataacgg aacaattata tgattttgat cataatgaaa
    46381 aagaatgcct gatattaatg aaattttctc atagaaagag aagcggaatt aacatcttgc
    46441 atagcagggc aaaataaaaa ccgaagccag ctacaacttc ggtttcttga ttaaggcagt
    46501 aattaagcag cggttttcag ctggctttac gctcatgtgc ctgacggtat gccaccaggt
    46561 cttcaatagt cacgagcgcc atattgtgtt tattggcaaa ctcaatgcac tctggtgcac
    46621 gcgccatcgt gccatcgtca ttagtcagct cacacagcac accagccggt ttaaagcctg
    46681 ccagcgtcat caaatcaata gtcgcttcag tatgaccgcc acgcgtcagt acaccacctg
    46741 cctgcgcgcg aagtgggaaa acgtggcccg ggcgattcag atcagaaggt tttgcgccat
    46801 cggcaatcgc tgcgcgaacg gtcgtaatac gatcagcggc agaaacaccg gtagtcacac
    46861 cttcagccgc ttcaatggtc acggtaaaac cggtgccata ggcgctggtg ttattttcta
    46921 ccatcattgg cagatcaagt tgtttacggc gatcttcagt gatgcacagg caaacaatac
    46981 cgctaccgtg gcgaatggtc agcgccatct gctcaacggt catggtttct gccgggaaga
    47041 tcatatcgcc ttcgttttca cggtcttcat catcaagcac cattacaccg cgtccttcac
    47101 gcagcgcagc cagtgcattt tcaacacgtt cgaaaggcgt accaaaagag gaaagtagcg
    47161 tctgattcat ggtaaaaaaa cctcactaaa attatggtta ccagaatcag ggcagtctta
    47221 ggagtggcgg catatagcca aaataacgtg agcgggtcca tgcccgacag aatcgttact
    47281 ctctcccatc cggactctaa ccgtcggccc cggaattaca ccggatctgc tgtcctttga
    47341 gttcgcaccc aaagcgctcg cgggctttca actgagttga tttaccgccg gtggggaatt
    47401 tcgccccgcc ctgagaataa gcggattcac tataacgcta atgattagcg gcagcaacgc
    47461 atagcttcac ataattctgg tttatgactt acccttatcg cactacaatg gtactcaaca
    47521 cctatcagta cagggaaact acaatgattg acccgaaaaa aattgagcaa atcgctcgcc
    47581 aggttcacga atcaatgcct aaaggaatca gggagttcgg ggaagatgtg gagaaaaaaa
    47641 tccgccaaac cctacaagct cagctgacgc gcctcgacct tgtaagccgt gaagagttcg
    47701 acgtccaaac gcaagtcctg ttacggactc gtgagaaact ggcgcggctt gaacaaagaa
    47761 tgagcgaact ggaaaatcgc agtactgaga ttaaaaagca gccagatcca gagactctcc
    47821 ccccaacgct gtaaacattt gtctttaatg cactgtaaat tatgaattaa tcttctaatt
    47881 tacagtgctt tctattacct tatcgcttaa ttccattcag aatatcatca caaaaatgaa
    47941 tgagtgcaat tgaaaatata attgttatgt attttttaag ttttacttaa tccatattta
    48001 gataaactaa tttcataaaa cccgaaaagt aaaatataat atttgaatga ttagcaatcc
    48061 ttaaataaca gttactaatc attcattttc tacttatatt tatataaagg aatattatgt
    48121 ctgcttttaa aaagtcactt cttgttgctg gcgtagcaat gattctttcc aataacgtat
    48181 ttgctgacga aggtcacggc attgttaaat ttaaaggtga agttatctct gctccatgtt
    48241 caattaaacc aggtgacgaa gacttaactg taaatctggg tgaagtagca gataccgttc
    48301 tgaaaagcga ccagaaatct ttggctgagc ctttcactat tcacctgcaa gactgcatgt
    48361 tgagccaggg tggaaccact tattcaaaag cgaaggttac tttcaccaca gcgaatacta
    48421 tgacgggtca gactgacctg ctgaaaaaca ccaaagaaac tgaaatcggt ggtgccacag
    48481 gcgttggcgt acgtattctg gatagccaga gcggcgaagt gactttaggt actccggtag
    48541 taattacatt caacaatact aacagctatc aggaactgaa cttcaaagct cgtatggaat
    48601 ccccgggcaa agatgctacc ccgggcaacg tttacgctca ggctgactat aagattgctt
    48661 acgagtaatc acattaggcc gggttcgccc ggccacatca ttaacggatt aatgataagt
    48721 ggaacagatg tataaaaaat taaaattaac cacaataagc gaattgatta aaaatattta
    48781 ttgttcatta tccgttctca tcattggttg tgcgtcagct tatgccgttg aattcaacaa
    48841 agatttaatc gaagccgaag atcgtgaaaa cgttaacctt tcccaatttg aaactgatgg
    48901 ccaattaccc gtaggtaaat actcattaaa cgctcttatt aataataaaa ggacgccaat
    48961 ccatctggac ctgcaatggg tattaattga taaccaaact gcagtttgtc tgactccaga
    49021 gcaattaaca ttattaggct ttactgatga gattattgaa gaggctcagc aaaacctgat
    49081 cgatggttgt taccctatcg aaaaagaaaa acaaattaca acttatctcg ataaagggaa
    49141 aatgcaatta tccatatctg cacctcaggc atggttaaaa tacaaagatg caaactggac
    49201 gcctcctgaa ctttgggatc atggtattgc tggggcattt cttgactaca atttatatgc
    49261 ctctcattat gcaccacatc agggcgataa ttcacaaaat ataagttcct atgggcaggc
    49321 tggggttaat cttggggcct ggcgcctgcg tactgattac cagtacgatc agtcatttaa
    49381 caatggcaaa agccaggcga acaacctgga ttttccgcgt atttatttgt ttcgcccaat
    49441 cccagcaatt aatgcaaaac taactatagg tcaatacgat actgaatcct ctattttcga
    49501 ctctttccat ttttctggcg tttcgttgaa aagcgatgag aatatgttgc cgcctgatct
    49561 acgtggttac gcaccgcaaa tcacgggtgt tgcacaaacg aatgcaaagg tcactgtctc
    49621 acagaacaac cgtattattt atcaagaaaa tgttcctcct ggcccatttg ctattactaa
    49681 tttattcaat acattacagg ggcaactgga cgtcaaggtt gaagaagagg acgggccagt
    49741 ctatatatca atgacactaa ccattacatg aattatcaga atgaaaaaga gagttatatt
    49801 gtcacgttta accagtatct tgaatca
//
LOCUS       DAJTZL010000007        46684 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.007, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000007 DAJTZL010000000
VERSION     DAJTZL010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 46684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46684
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.007"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..135)
                     /gene="tabA"
                     /locus_tag="OO544_000384"
     CDS             complement(<1..135)
                     /gene="tabA"
                     /locus_tag="OO544_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HCR6766082.1"
                     /translation="MIIGNIHNLQPWLPQELRLAIEHIKAHVTAETPKGKHDIEGNRL
                     F"
     gene            complement(280..873)
                     /locus_tag="OO544_000385"
     CDS             complement(280..873)
                     /locus_tag="OO544_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR6766083.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVAEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLAEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAVQYYHAAETAIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTILKE"
     gene            944..1657
                     /gene="bdcA"
                     /locus_tag="OO544_000386"
     CDS             944..1657
                     /gene="bdcA"
                     /locus_tag="OO544_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR6766084.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     EHLAQKTGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMAREL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            1788..2183
                     /locus_tag="OO544_000387"
     CDS             1788..2183
                     /locus_tag="OO544_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCR6766085.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPENQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEQ"
     gene            2464..2598
                     /gene="pyrL"
                     /locus_tag="OO544_000388"
     CDS             2464..2598
                     /gene="pyrL"
                     /locus_tag="OO544_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HCR6766086.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            2602..3537
                     /gene="pyrB"
                     /locus_tag="OO544_000389"
     CDS             2602..3537
                     /gene="pyrB"
                     /locus_tag="OO544_000389"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HCR6766087.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            3550..4011
                     /gene="pyrI"
                     /locus_tag="OO544_000390"
     CDS             3550..4011
                     /gene="pyrI"
                     /locus_tag="OO544_000390"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HCR6766088.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            4084..4470
                     /gene="ridA"
                     /locus_tag="OO544_000391"
     CDS             4084..4470
                     /gene="ridA"
                     /locus_tag="OO544_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HCR6766089.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(4676..7372)
                     /gene="mgtA"
                     /locus_tag="OO544_000392"
     CDS             complement(4676..7372)
                     /gene="mgtA"
                     /locus_tag="OO544_000392"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HCR6766090.1"
                     /translation="MFKEIFTRLIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(7513..7566)
                     /gene="mgtL"
                     /locus_tag="OO544_000393"
     CDS             complement(7513..7566)
                     /gene="mgtL"
                     /locus_tag="OO544_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HCR6766091.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            7751..8698
                     /gene="treR"
                     /locus_tag="OO544_000394"
     CDS             7751..8698
                     /gene="treR"
                     /locus_tag="OO544_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator TreR"
                     /protein_id="HCR6766092.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMESQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            8817..10238
                     /gene="treB"
                     /locus_tag="OO544_000395"
     CDS             8817..10238
                     /gene="treB"
                     /locus_tag="OO544_000395"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HCR6766093.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            10288..11943
                     /gene="treC"
                     /locus_tag="OO544_000396"
     CDS             10288..11943
                     /gene="treC"
                     /locus_tag="OO544_000396"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HCR6766094.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVQHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            12337..14475
                     /gene="nrdD"
                     /locus_tag="OO544_000397"
     CDS             12337..14475
                     /gene="nrdD"
                     /locus_tag="OO544_000397"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HCR6766095.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSRESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            14633..15097
                     /gene="nrdG"
                     /locus_tag="OO544_000398"
     CDS             14633..15097
                     /gene="nrdG"
                     /locus_tag="OO544_000398"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HCR6766096.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDKIIKDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(15142..15528)
                     /gene="cybC"
                     /locus_tag="OO544_000399"
     CDS             complement(15142..15528)
                     /gene="cybC"
                     /locus_tag="OO544_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HCR6766097.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVVEKADN
                     ATQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(15712..17064)
                     /gene="pmbA"
                     /locus_tag="OO544_000400"
     CDS             complement(15712..17064)
                     /gene="pmbA"
                     /locus_tag="OO544_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HCR6766098.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGLADKELLAFDAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLLPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            17158..17709
                     /locus_tag="OO544_000401"
     CDS             17158..17709
                     /locus_tag="OO544_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HCR6766099.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            complement(17792..19165)
                     /gene="mpl"
                     /locus_tag="OO544_000402"
     CDS             complement(17792..19165)
                     /gene="mpl"
                     /locus_tag="OO544_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HCR6766100.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLEPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVASADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            19341..20339
                     /gene="fbp"
                     /locus_tag="OO544_000403"
     CDS             19341..20339
                     /gene="fbp"
                     /locus_tag="OO544_000403"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HCR6766101.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENAQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEY
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            complement(20372..21367)
                     /gene="yjfF"
                     /locus_tag="OO544_000404"
     CDS             complement(20372..21367)
                     /gene="yjfF"
                     /locus_tag="OO544_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
                     /protein_id="HCR6766102.1"
                     /translation="MIKRNLPLMITIGVFVLGYLYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVIGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPIYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQQ"
     gene            complement(21354..22376)
                     /locus_tag="OO544_000405"
     CDS             complement(21354..22376)
                     /locus_tag="OO544_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6766103.1"
                     /translation="MPQSLPDTTPPKRRFHWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSGVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSRDKT"
     gene            complement(22390..23892)
                     /locus_tag="OO544_000406"
     CDS             complement(22390..23892)
                     /locus_tag="OO544_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCR6766104.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQAISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFFLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHVLQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAQLSVPAIMNAIAA"
     gene            complement(24032..24988)
                     /gene="ytfQ"
                     /locus_tag="OO544_000407"
     CDS             complement(24032..24988)
                     /gene="ytfQ"
                     /locus_tag="OO544_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HCR6766105.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTGGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMMDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            25298..25828
                     /gene="ppa"
                     /locus_tag="OO544_000408"
     CDS             25298..25828
                     /gene="ppa"
                     /locus_tag="OO544_000408"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HCR6766106.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(26207..26548)
                     /locus_tag="OO544_000409"
     CDS             complement(26207..26548)
                     /locus_tag="OO544_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HCR6766107.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(26551..30330)
                     /gene="tamB"
                     /locus_tag="OO544_000410"
     CDS             complement(26551..30330)
                     /gene="tamB"
                     /locus_tag="OO544_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HCR6766108.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGNVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEVAQEEVVEPKIENPQPDEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDIILNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(30327..32060)
                     /gene="tamA"
                     /locus_tag="OO544_000411"
     CDS             complement(30327..32060)
                     /gene="tamA"
                     /locus_tag="OO544_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HCR6766109.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQILDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSDWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGGGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            32266..32904
                     /gene="msrA"
                     /locus_tag="OO544_000412"
     CDS             32266..32904
                     /gene="msrA"
                     /locus_tag="OO544_000412"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCR6766110.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            33227..34570
                     /locus_tag="OO544_000413"
     CDS             33227..34570
                     /locus_tag="OO544_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCR6766111.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(34650..34856)
                     /locus_tag="OO544_000414"
     CDS             complement(34650..34856)
                     /locus_tag="OO544_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HCR6766112.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            35181..35735
                     /locus_tag="OO544_000415"
     CDS             35181..35735
                     /locus_tag="OO544_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HCR6766113.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLQKLLK"
     gene            complement(35825..36736)
                     /locus_tag="OO544_000416"
     CDS             complement(35825..36736)
                     /locus_tag="OO544_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6766114.1"
                     /translation="MLPRIRHNNFIGAVELFVKSSYTKTHSNNFFNNIQHAFKKKDWI
                     SNYDSLLTLREFFRCATQIDKNSYQVLSSKNGTVNAMDKFLISFSLKDNGAEYTMTLR
                     GSGFEYEEIPITINEYNSFMDFKNREFPLEQNRRLYACDILQKKQSDIPKRIKGYIHQ
                     AIGDVSLGYALLEDIVSKLKRGKFELQIPGGGIKECDGWYIHEKIIDDNFAIVIESLG
                     FALKIYGGDERFRNGSSVVLEDEDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAADS
                     NITKEKICKKYKSTIEAFKKEQLALPV"
     gene            complement(36948..37688)
                     /gene="cysQ"
                     /locus_tag="OO544_000417"
     CDS             complement(36948..37688)
                     /gene="cysQ"
                     /locus_tag="OO544_000417"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HCR6766115.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            37878..39821
                     /gene="cpdB"
                     /locus_tag="OO544_000418"
     CDS             37878..39821
                     /gene="cpdB"
                     /locus_tag="OO544_000418"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HCR6766116.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKTGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINTNAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAADKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(39939..40319)
                     /locus_tag="OO544_000419"
     CDS             complement(39939..40319)
                     /locus_tag="OO544_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCR6766117.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            40408..41268
                     /gene="qorB"
                     /locus_tag="OO544_000420"
     CDS             40408..41268
                     /gene="qorB"
                     /locus_tag="OO544_000420"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HCR6766118.1"
                     /translation="MIAITGATGQLGHYIIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAAAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGRPTTTLAE
                     SVSHLFNVNN"
     gene            41376..42341
                     /locus_tag="OO544_000421"
     CDS             41376..42341
                     /locus_tag="OO544_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR6766119.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALVCIGIGLACVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            42449..43111
                     /gene="ytfE"
                     /locus_tag="OO544_000422"
     CDS             42449..43111
                     /gene="ytfE"
                     /locus_tag="OO544_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HCR6766120.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(43156..44568)
                     /gene="cycA"
                     /locus_tag="OO544_000423"
                     /gene_synonym="dagA"
     CDS             complement(43156..44568)
                     /gene="cycA"
                     /locus_tag="OO544_000423"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HCR6766121.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(44877..45497)
                     /gene="fklB"
                     /locus_tag="OO544_000424"
     CDS             complement(44877..45497)
                     /gene="fklB"
                     /locus_tag="OO544_000424"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR6766122.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            45716..46354
                     /gene="ytfB"
                     /locus_tag="OO544_000425"
     CDS             45716..46354
                     /gene="ytfB"
                     /locus_tag="OO544_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HCR6766123.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNDPDQVAPVAPEPIQ
                     EGQPEEQPQTTQTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GAGKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            complement(46338..>46684)
                     /locus_tag="OO544_000426"
     CDS             complement(46338..>46684)
                     /locus_tag="OO544_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR6766124.1"
                     /translation="PWFLKIVHSENQSKGVHYAKSLRLLEINHAHLQLMESLLDEGKK
                     HNIFKPDIDPLQVNINIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILS
                     WLLVDPSSTAHE"
BASE COUNT        11455 a        12415 c        11632 g        11182 t
ORIGIN      
        1 aaacagacga ttgccttcga tatcgtgctt gccctttggc gtttctgccg taacgtgtgc
       61 tttgatatgc tcaatcgcca ggcgtaactc ctggggtagc cacggctgaa gattatgaat
      121 atttccgatg atcatctttt ccactcctta attaaaacaa tgtttcattt ccatcgttat
      181 acgaaaagat gatcaattcc gctagcctta atgcgtgaat tattgtctta tcacaactta
      241 tcggcaggcg cgttcccgcc tttcgggtca aggcccgcat cactccttga gaatagtttt
      301 tatcgcttcc cccgccagtg cagctgttgc acagagttgt tcgatggagt gcccctcccg
      361 agccttcgca gaaagccctg acatcacggt actcataaaa tcagtcacac attgtgcgct
      421 ttgtggatgc cgcctggcga tatagtcata aatggccgtt tctgcggcgt gataatattg
      481 aacggcaata tcacgcgctt gtggatcatg actatgaatg ccttcaagaa ccatacagcc
      541 agcgcagccg ccgttttggc tatatctgcg cgccgcttct tttaatacct cagccaggca
      601 ctcccctacc ggacgatcat cacgaagaat atcggcaagc ggaatagctt ccgtaccgac
      661 atattcattg agtacacggc taaataaccc agccttactg ccaaaagccg cgtagaggct
      721 cggggggtta ataccaagat aatcagtaac ttcagcaaca ctgacagcat cgaaaccttt
      781 ttggtgaaaa agcacttttg ccgcagagac tgcctgctca ggagcgaaac gtctgggacg
      841 acctggaacg cgagattgtt ttttagtgac cattacaaaa cttgttgaca gaaagttaaa
      901 acagttttgt aatgcatgtt acataataaa tcaaggagtc cttatgggcg cttttacagg
      961 taagacagtt ctcatcctcg gtggcagtcg tggtatcggt gccgctatcg tacgtcgttt
     1021 cgtcaccgat ggggccaatg tacgattcac ctatgcgggg tcgaaagatg ccgctgaaca
     1081 cctggcacaa aagactggag cgacagcagt attcacagat agtgctgaca gagacgctgt
     1141 cattgatgtc gttcgtaaga gcggcgcatt ggatatcctg gtggtaaatg caggtattgg
     1201 cgtctttggc gatgccctgg aattgaatgc cgacgatatt gatcgccttt tcaaaatcaa
     1261 tattcatgct ccttatcatg cctctgttga agccgcccgg cagatgcccg aaggcgggcg
     1321 catcttaatc atcggctccg tgaatggcga tcgtatgcct gttgcaggca tggctgctta
     1381 tgccgccagt aaatctgccc tgcaaggcat ggcgcgcgag ctggcccgtg atttcggacc
     1441 gcgtgggatc accatcaacg tcgtccagcc agggccaatt gataccgacg ctaatcccgc
     1501 caacgggcca atgcgcgata tgttgcatag ttttatggct atcaaaagac atgggcaacc
     1561 ggaagaggtc gctggtatgg tcgcatggtt agcagggcca gaagccagtt ttgttaccgg
     1621 cgcgatgcat accattgatg gcgcgtttgg cgcataaccg actacgctca attaagccca
     1681 gccatttccc atgatgtctg ggttttgttt actcacgtcg tccgctaaaa gcggctcctg
     1741 gtaaatataa atcttctgtt catgtcgtaa atcttgagga taaccaaatg gtagaaagaa
     1801 ccgctgtttt ccctgcaggc cgacattcac tgtatgctga acatcgttat tctgcggcta
     1861 ttcgttccgg cgatttgctg tttgtttccg ggcaagtagg aagtcgagag gacggaacac
     1921 cagaacccga ttttcagcaa caagtcagac tggcatttga taatttgcat gcgaccctgg
     1981 ctgctgcggg atgcactttt gacgatatca ttgatgttac gagcttccat accgatccag
     2041 aaaaccaatt tgaagacatc atgacggtga aaaatgaaat atttagcgcc ccaccttatc
     2101 caaactggac ggcggtaggt gttacatggc tggcaggctt tgattttgaa attaaagtga
     2161 tagcgcgcat ccctgagcag taagcaatag tgttagccgt tcgttttcac actccgccct
     2221 ataagtcgga tgaatggaat aaaatgcata tctgattgcg tgaaagtgaa aaaggaaaaa
     2281 gcaggaaatg tctgcaatta ttgataccga acgaccgttc ccctgcagaa tcacaacaaa
     2341 taaaaatgca tgtattttga cttataattc aaataaaccg tttgcgctga caaaatattg
     2401 catcaaatgc ttgcaccgct tctgacgatg agtataatgc cggacaattt gccgggagga
     2461 tgtatggttc agtgtgttcg acattttgtc ttaccgcgtc tgaaaaaaga cgctggcctg
     2521 ccgtttttct tcccgttgat cacccattcc cagcccctca atcgaggggc ttttttttgc
     2581 ccaggcgtca ggagataaaa gatggctaat ccgctatatc agaaacatat catttccata
     2641 aacgacctta gtcgcgatga ccttaatctg gtgctggcga cagcggcgaa actgaaagca
     2701 aacccgcaac cagagctgtt gaagcacaaa gtcattgcca gctgtttctt cgaagcctct
     2761 acccgtaccc gcctctcttt cgaaacttcc atgcaccgcc tgggtgccag cgtggtgggc
     2821 ttctccgaca gcgccaatac atcactgggt aaaaagggcg aaacgctggc cgataccatt
     2881 tcggttatca gcacttacgt cgatgcgata gtgatgcgtc atccgcagga aggtgcggcg
     2941 cgcctggcca ccgagttttc cggcaatgta ccggtactga atgccggtga tggctccaac
     3001 caacatccga cgcaaacctt gctggactta ttcactattc aggaaaccca ggggcgtctg
     3061 gacaatctcc acgtcgcaat ggttggtgac ctgaaatatg gccgcaccgt tcactccctg
     3121 actcaggcgt tagcgaagtt cgacggcaac cgtttttact tcatcgcgcc ggacgcgctg
     3181 gcaatgccgc aatacattct ggatatgctt gatgaaaaag ggatcgcctg gagtctgcac
     3241 agctctattg aagaagtgat ggcggaagta gacatcctgt acatgacccg cgtgcaaaaa
     3301 gagcgtctgg acccgtccga gtacgccaac gtgaaagcgc agtttgttct tcgcgccagc
     3361 gatctccaca acgccaaagc caatatgaaa gtgctgcatc cgctgccgcg tgttgatgag
     3421 attgcgacgg atgttgataa aacgccacac gcctggtact tccagcaggc aggcaacggg
     3481 attttcgctc gccaggcgtt actggcactg gttctgaatc gcgatctggt actgtaaggg
     3541 gaaatagaga tgacacacga taataaattg caggttgaag ctattaaacg cggcacggta
     3601 attgaccata tccccgccca gatcggtttt aagctgttga gtctgttcaa gctgaccgaa
     3661 acggatcagc gcatcaccat tggtctgaac ctgccttctg gcgagatggg ccgcaaagat
     3721 ctgatcaaaa tcgaaaatac ctttttgagt gaagatcagg tagatcaact ggcattgtat
     3781 gcgccgcaag ccacggttaa ccgtatcgac aactatgaag tggtgggtaa atcacgcccc
     3841 agtctgccgg agcgcatcga caatgtgctg gtctgcccga acagcaactg tatcagccat
     3901 gccgaaccgg tttcttccag ctttgccgtg cgaaaacgcg ccaatgacat cgcgctcaaa
     3961 tgcaaatact gtgaaaaaga gttttcccat aatgtggtgc tggccaatta attgcggttg
     4021 gtaataaaag tctggctccc tataatgagc cagacttttt accgctgtaa taaaggagaa
     4081 atcatgagca aaactatcgc gacggaaaat gcaccggcag ctatcggtcc ttacgtacag
     4141 ggcgttgatc tgggcaatat gatcatcacc tccggtcaga tcccggtaaa tccgaaaacg
     4201 ggcgaagtac cggcagacgt cgctgcacag gcacgtcagt cgctggataa cgtaaaagcg
     4261 atcgtcgaag ccgctggcct gaaagtgggc gacatcgtta aaaccaccgt gtttgtgaaa
     4321 gatctgaacg acttcgcaac cgttaatgct acctacgaag ccttcttcac cgaacacaat
     4381 gccaccttcc cggcacgttc ttgcgttgaa gttgcccgtc tgccgaaaga cgtgaaaatt
     4441 gagatcgaag cgatcgctgt tcgtcgctaa tcttgatgga aatccgggct atcatgcccg
     4501 gattaagtct gatgacaaac acaaaatcgc ctgatgcgct acgcttatca ggcctacgtg
     4561 attcctgcaa tttattgaat ttgttggccg gataaggcat ttacgccgca tccggcatga
     4621 acaaaactca ctttgtctgc aatctgaatc caggccacca tgcccagttg attctttatt
     4681 gccagccgta acgacggcta taaaatcctt tcaccagttg ggttaacgtc atataccctg
     4741 ccagaatcgc aaccagccac gggaaatagc ttaacggcag cgcctgcagt tgcagataac
     4801 tggccagcgg tgaaaacggc aatgcgatcc cgacaatcat cacgatcacg gtcatgatca
     4861 ttaacggcca cgatgcacag ctctgaataa acggcacacg gcgggtgcgg atcatatgca
     4921 caatcagcgt ttgcgacagc aagcccacca caaaccatcc cgactggaac agcgtttgcg
     4981 tttccggcgt gttggcatgg aatacccacc acatcaggca aaacgtcaga atatcgaaga
     5041 tcgagctgat cggtccgaag aagatcataa agcgccccag atccgccgga ttccaacgct
     5101 gcggcttttg aatttgctcg tcgtcgacgt tatcaaacgg gatcgccacc tgtgacacat
     5161 catacagcag gttctgaata agcaagtgta acggcaacat cggcaggaag ggcaagaaag
     5221 cactcgccac cagcacgctg aacacattac cgaagttgga gctcgccgtc attttgatgt
     5281 atttcagcat gttggcgaaa gtgcgacgtc cttcaataac cccctcttcc agcaccatca
     5341 ggcttttttc cagcaggatg atatcagccg cttcacgggc aatatctacc gcgccgtcca
     5401 cagaaatgcc gatatccgca gcgcgtaagg cgggcgcatc attaatacca tcgcccataa
     5461 agccaaccac atgcccttcg cgcttcagta aggtcacaat gcgttcttta tgcatcggcg
     5521 tcagacgagc aaacagcgtg gtgcgctgtg cgagatttgc cagttcgtcg tcagatagcg
     5581 tttcaatatc actaccaatg accacctctc ccgcgtccag ccccacttca tggcacactt
     5641 tcgctgcaac taactcactg tcgccagtaa ggatttttac ggtaattcca ctcgctttta
     5701 atgccttcag tgccggagca gttgtctctt ttggcggatc aaggaaagca atatatcctt
     5761 cgaggatcag gtcggattca tccgcccgct ggtaatctcc ttcacgcgct ggcaggtatt
     5821 tcgtcgccac cgcaaccacg cgcagcccct gacgattcag cgtatcagta acccgcttaa
     5881 tcttacgcag catgatgtca tcgagcggca caatctcgcc attgtgacgc acctgcgaac
     5941 acacattgag gatttcctgc aatgcacctt tgcaaaccag ctgatggtgc tcggtatttt
     6001 ctgccactac caccgacatc cggcgacgct cgaaatcaaa cggaatctca tcaatttttt
     6061 gccaacggct ggccagagag cgcgctgact cttcatccgt accttcgagc accgctgtat
     6121 cgagtaggtt tttaagtccg gtctgataat gactgttcaa ccacgcgcta tgcagcacgc
     6181 gttcgctggt tttaccggag atatcggtat gattctccag cacaattttg tcctgtgtca
     6241 gggtgccagt tttatcagtg cacagaatat ccattgcgcc aaagttctga atagcatcca
     6301 gatgtttgac gatcactttc tgtttcgaca gctttactgc gccgcgcgcc agcgtcgagg
     6361 taacaatcat cggcaacatt tccggcgtta agcctaccgc gaccgaaagc gcaaacagtg
     6421 ccgcttccca ccagtcgcct ttggtgtaac cgttgataag caacaccacc ggagccatca
     6481 ccagcataaa gcgaatcagc agcatactga cgcggctgat cccctgctga aaggcattcg
     6541 gctcgctttc ctgctcacta acacgccccg ccagttgacc aaaccaggtg ttggctcctg
     6601 tagcaatcac cattgcttgt gccgtgccgc tcaccacggt ggtgcccata aaacacaggg
     6661 tgtcacactc cagcggattg ctgtgctccg gctggcgagt ggtagcggct ttttccacgg
     6721 gcagagactc accggttaac gacgcctgag cgacgaacag atcccgcgcc tgcaagatac
     6781 gtaaatctgc cgggatcata tcccccgccg ccagttttat aatatcgccg ggcaccagct
     6841 ggtcgatcgg gatctccagc cagccatttt cgcctttgtc gttaattacg cgcagcaccg
     6901 tcgcggtatt gctgaccatc gctttcaggg catctgccgc tttagtggaa cgtgcttcct
     6961 gaataaagtt cagcaacgta gaaatagcga ccattagcgc gataacgccc gcggcaaata
     7021 aatcttcagt ggcgtaagaa atagcgccga gaatggtgag taaaatatta aatgggttgc
     7081 gatagcagac ccataaatgt acccaccacg gcgacggttg ttgtgcgggt aatttatttt
     7141 caccatgttg ttcgcgggca gattccactt ccgcctgatt taacccttcc ggatgggtgt
     7201 cgaacgtttt ccacaattct tcttcgggca tcaccgccat tttcaggcaa tgcgcactta
     7261 aggacggcgg gaccgccgta ttcactgtct gctgcgcgcc aggcaatgga tcgcgatgaa
     7321 ccagacggga aggtaaatgg cgaatgagcc gggtaaaaat ttctttaaac ataaggagtc
     7381 cctccgcact gtctgaaaca gtacagaaat gtcttcaggc gtggcgaagc cttacctgaa
     7441 ggtaataaag ctgagcaggg acgttaaaac gtcacggcct tacgcatccg gtagcgccgt
     7501 gaaaagacgg gcttaccgga aaagttttaa tctccgtcga gggagaggcg tgggatcagg
     7561 ttccatataa cctccggtaa gtgaaaaaag cgccgcaaat aataaggaga atccaataat
     7621 attcgtagca tttatggcga tataatactt gctggcaact aaaccaacga taaaccagac
     7681 tttacccatt gctgaatgca cgggtaaagt taggctcaaa taattaaaca acacgttaca
     7741 ggacaacagg atgcaaaatc ggctgactat caaagacatc gcacgcttaa gcggcgtggg
     7801 gaaatctaca gtttcccggg tgctgaataa cgaaagcggc gtgagccagc gcacccgcga
     7861 gcgtgttgaa gcagtgatga atcagcatgg attttcccct tcccgctctg cgcgcgctat
     7921 gcgtgggcaa agcgataaag tggtcgccat cattgttacc cgtctggatt cgttgtcaga
     7981 aaatctcgcc gttcaaacca tgctgccagc gttctatgaa caaggttacg acccaatcat
     8041 gatggaaagc cagttttccc cgcaattagt tgccgaacat ttgggggtgc tgaaacggcg
     8101 taatatcgac ggcgtagtgc tgttcggttt tactggcata acagaagaaa tgttagccca
     8161 ctggcagtca tcgctggttc tgctggcgcg tgacgcaaaa ggctttgctt cggtctgtta
     8221 tgacgacgaa ggggcaatca agatcctgat gcaacggctg tatgaccagg ggcatcgtaa
     8281 tatcagttat ctcggcgtgc cgcatagtga cgtgacaacc ggtaagcgac gtcacgaagc
     8341 ctacctggcg ttctgcaaag cgcataaact gcatcccgtt gccgctctgc cagggcttgc
     8401 tatgaagcaa ggctatgaga acgtggcaaa agtgattacg cctgaaacta ctgcgttact
     8461 gtgcgcaacc gacacgctgg cacttggcgc aagtaaatac ctgcaagagc aacgcatcga
     8521 caccttgcaa ctggcgagcg tcggtaatac accgttaatg aaattcctcc atccggagat
     8581 cgtcaccgta gatcccggtt acgccgaagc tggacgccag gcggcctgcc aattgatcgc
     8641 acaggtaacc gggcgcagcg aaccgcaaca aatcatcatc cccgccaccc tgtcctgatc
     8701 gtttcctgaa cgataaattg tgatcttcgc tgcatttcgg gaacgttccc gtttttaaat
     8761 ttttccgcgc aatatattct gcagccaacc aaaaatgtca tctgccatgg ggctttatga
     8821 tgagcaaaat aaaccaaacg gatatcgatc ggttgattga actggtcggc gggcgcggca
     8881 atattgcgac ggtgagccac tgtattactc gcctgcgctt tgtcctcaac caaccggcca
     8941 atgccagacc gaaagaaatt gagcaactcc ccatggtgaa aggctgtttc accaatgccg
     9001 ggcaatttca ggtggtgatt ggcaccaacg tgggtgatta ctatcaagca ctaatagcgt
     9061 caaccggaca ggcgcaggtt gataaagagc aggtaaaaaa agccgcccgg cagaatatga
     9121 aatggcatga gcagttgatc tctcatttcg cggagatctt cttcccgttg ctgcccgcgt
     9181 tgattagcgg cggtttgatc ctcggttttc gcaatgtgat cggcgatttg cccatgagca
     9241 acggtcagac gctggcgcaa atgtaccctt ccctgcaaac gatctacgat tttctgtggt
     9301 tgatcggtga agcgattttc ttctacctgc cggtcgggat ttgctggtca gcggtgaaaa
     9361 aaatgggcgg cacgccgatc cttggtatcg tgcttggcgt gacactggtt tccccccagc
     9421 tgatgaacgc ttatctgctc gggcagcagc tgccggaagt gtgggacttt ggcatgttca
     9481 gcatcgccaa agtgggctat caggcgcagg tgatcccggc actgttagcc gggctggcgc
     9541 tgggcgttat tgaaactcgc cttaaacgca tcgtaccgga ttacctctat ctggtggtgg
     9601 tgcccgtctg ttcgctgatc ctcgcggtgt tcctcgccca tgcgctgatt ggtccgtttg
     9661 gtcgcatgat tggcgatggc gttgcctttg cggtacgtca cctgatgacc ggcagctttg
     9721 ctccgattgg tgcggcattg tttggcttcc tgtacgcgcc gctggtgatc accggcgtac
     9781 accagaccac ccttgctatt gatttgcaga tgattcaaag catgggtggc acgccagtgt
     9841 ggccgctgat tgcgctgtcg aatatcgctc agggctccgc cgtgataggc attatcattt
     9901 ccagccgcaa gcacaatgaa cgcgagatct ccgtgcctgc cgctatctcc gcctggcttg
     9961 gggtcactga gcctgcaatg tacggcatca acctgaaata tcgcttcccg atgctgtgcg
    10021 cgatgattgg ttctggtctg gcaggattgc tatgcggcct gaacggcgtt atggcgaatg
    10081 gtatcggcgt agggggcctg ccgggaattc tctcgattca accgagctac tggcaggtat
    10141 ttgcgctggc aatggctatc gccatcatca tcccgattgt actcacctcg tttatctatc
    10201 agcggaaata ccgcttgggc acgctggaca ttgtttaatt ttcttcgggg cgcaattgcg
    10261 ccccctcgca tttgcaggaa taacgtaatg actaatcttc cccactggtg gcaaaacggc
    10321 gttatctacc agatttatcc aaagagtttt caggacacca cgggtagcgg caccggcgat
    10381 ttacgtggcg ttatccaacg cctggactat ctgcataaac tgggcgttga tgccatctgg
    10441 ctaaccccct tttatgtctc tccccaggtc gataacggtt acgatgtagc gaactatacg
    10501 gcgattgatc ccacctacgg cacgctggac gattttgacg aactggtgac gcaggcaaaa
    10561 tcgcgcggga ttcgtatcat tctcgatatg gtgtttaacc atacctctac ccaacatgcc
    10621 tggtttcgcg aggcgctgaa caaagaaagc ccttaccgcc agttttatat ctggcgcgat
    10681 ggcgaaccag aaacgccacc gaacaactgg cgttcaaaat ttggcggtag tgcgtggcgc
    10741 tggcatgcgg aaagcgaaca gtactatttg catctctttg caccagaaca ggcggatctc
    10801 aactgggaga atccagcggt acgcgcagag ctgaaaaaag tctgtgaatt ctgggccgat
    10861 cgtggggtcg acgggttgcg cctggatgtg gtgaatctga tctccaaaga cccgcgtttc
    10921 cctgaagacc tggatggcga cgggcgtcgc ttctacaccg acgggccacg agcacacgag
    10981 tttttgcacg agatgaaccg cgatgtgttt acgccacgcg ggttaatgac cgtaggtgaa
    11041 atgtcctcca ccagccttga gcattgccag cgatacgcgg cactgacagg cagtgaattg
    11101 tcgatgacct ttaattttca tcacctgaag gtcgattatc ccggtggtga aaaatggacg
    11161 ctggctaaac ctgactttgt ggcgttgaaa acattgttcc gccactggca acaaggaatg
    11221 cacaacgtgg catggaatgc cttgttctgg tgtaaccacg atcagccgcg cattgtttct
    11281 cgctttggtg atgaaggtga ataccgcgtg cctgcggcaa aaatgctggc gatggtgcag
    11341 catggcatgc agggaacgcc gtatatctac cagggcgaag agattggcat gaccaacccg
    11401 catttcacgc gcattactga ctatcgcgac gtggagagcc tcaatatgtt tgccgagctg
    11461 cgcaacgatg gtcgtgatgc cgacgagtta ttggcaatcc tcgccagtaa atcccgtgac
    11521 aacagccgca cgcccatgca atggagcaac ggcgataatg ccgggtttac ggctggcgaa
    11581 ccgtggattg gcctgggcga taactatcaa caaatcaacg tagaagccgc gctggccgat
    11641 gactcctcgg tgttttacac ctaccaaaag ttaatcgcac tgcgtaagca agaagccatc
    11701 ctgacatggg gcaattacca ggatctgctg ccaaacagcc ctgtattgtg gtgctatcgc
    11761 cgtgaatgga aggggcaaac cttgctggtc attgccaacc ttagccgtga gatccaaccc
    11821 tggcagccag ggcaaatgcg cggcaactgg cagcttgtga tgcataacta cgaagaagcc
    11881 tcaccacaac cctgtgccat gaatttacgg ccttttgagg ctgtctggtg gttacagaag
    11941 taaatcttcc ctaagccccg gtaatgccgg tcattccggg gttttgtctg tcagcatgtt
    12001 gtttttgttg atttttcaac cagcaaattc attaaaaaat ttacataacg ctgtagcgcc
    12061 cgtcatccgt acgctctgct ttttactttg agctacatca aaaaaagtta aaacatcctt
    12121 gatgcaaagc actatatata gactttaaaa tgcgtcccaa cccaatatgt tgtattaatc
    12181 gactataatt gctactacag ctccccacga aaaaggtgcg gcgttgtgga taagcggatg
    12241 gcgattgcgg aaagcaccgg aaaacgaaac gaaaaaaccg gaaaacgcct ttcccaattt
    12301 ctgtggataa cctgttctta aaaatatgga gcgatcatga caccgcatgt gatgaaacga
    12361 gacggctgca aagtgccgtt taaatcagag cgcatcaaag aagcgattct gcgtgcagct
    12421 aaagcagcgg aagtcgatga tgccgattat tgcgccactg ttgccgcggt tgtcagcgag
    12481 cagatgcagg gccgcaacca ggtggatatc aacgagatcc agaccgcagt tgaaaaccag
    12541 ctgatgtcgg gtccatacaa acaactggct cgtgcttaca tcgagtaccg tcacgatcgc
    12601 gacattgaac gtgaaaaacg cggtcgcctg aaccaggaga tccgtggtct ggtcgagcag
    12661 accaacgcct cgttactcaa cgaaaacgcc aacaaagaca gcaaggtgat tccaacccag
    12721 cgcgacctgc tggccgggat cgtggctaaa cactatgcac gccagcacct gctgccgcgt
    12781 gacgtggtgc aggcacatga gcgtggcgat attcactatc acgatctcga ttactcgccg
    12841 ttcttcccga tgttcaactg catgttgatc gacctgaaag gcatgctgac ccagggcttt
    12901 aaaatgggga acgccgagat tgaaccgccg aagtcgatct ctacggcaac cgcggtaact
    12961 gcgcagatta ttgctcaggt cgccagccat atttatggcg gcaccaccat taaccgtatc
    13021 gatgaagtgc tggcgccgtt tgttaccgcc agctacaaca aacatcgcaa gaccgcagaa
    13081 gagtggaaca tcccggacgc cgaaggctac gctaactctc gtaccatcaa agagtgctac
    13141 gatgccttcc agtcgctgga gtacgaagta aacaccctgc acaccgccaa cggtcagacg
    13201 ccgtttgtaa cctttggttt tggtctgggc accagcaggg aatcgcgcct gattcaggaa
    13261 tcgatcctgc gtaaccgtat cgcaggcctc ggtaaaaacc gtaaaactgc ggtgttcccg
    13321 aaactggtgt ttgcgattcg cgatggcctg aaccataaaa aaggcgatcc gaactacgac
    13381 atcaaacagc tggcgctgga gtgcgcaagc aagcgcatgt atccggatat cctgaactac
    13441 gatcaggtag tgaaagtcac cggttcgttt aaaaccccga tgggctgccg cagcttcctc
    13501 ggcgtgtggg aaaatgaaaa cggcgagcag atccacgatg gtcgtaacaa cctcggcgtg
    13561 atcagcctga acctgccgcg tattgctctg gaagcaaaag gcgatgaagc caccttctgg
    13621 aagctgctgg atgaacgtct ggtgctggca cgtaaggcac tgatgacccg tatcgctcgt
    13681 ctcgaaggcg tgaaagcgcg cgtcgccccg atcctctata tggaaggtgc ttgtggcgta
    13741 cgtctgaatg ctgacgatga tgtttctgaa atcttcaaaa acggtcgtgc gtctatttcg
    13801 ctgggttaca tcggcatcca cgaaaccatt aacgcgctgt tcggcggcga gcatgtttac
    13861 gacaacgagc agcttcgcgc gaaaggtatc gccattgttg aacgtctgcg tcaggcagtg
    13921 gatcagtgga aagaagaaac gggttatggt ttcagtctct acagcacgcc gagtgaaaac
    13981 ctgtgcgacc gcttctgccg tctcgatact gctgagtttg gcgtggtgcc gggcgtgacc
    14041 gataaaggtt actacaccaa cagtttccac cttgatgtgg agaagaaggt gaacccgtac
    14101 gacaagatcg actttgaagc gccttacccg ccgctggcga acggtggttt catttgctac
    14161 ggcgagtatc caaacattca gcacaacctg aaagcgctgg aagatgtctg ggattacagc
    14221 tatcagcatg taccgtatta cggcaccaat acaccgattg atgagtgcta cgagtgtggc
    14281 tttaccggtg agttcgagtg caccagcaaa ggcttcactt gcccgaaatg tggtaaccat
    14341 gacgcctccc gtgtgtcggt aactcgccgc gtgtgcggat atttaggtag cccggatgcg
    14401 cgtccgttta acgccggtaa gcaggaagaa gttaagcgcc gcgttaaaca tttggggaat
    14461 gggcagatag gttaatcttc actctgcgtt aagcaaatgc cggatgcggc gtaaacgcct
    14521 tatccggcct acatttggca acggctgtag gcctgataag acgcgacagc gtcgcatcag
    14581 gcattgatta ccggatgcag cctacacgcg gactgataag ccaggcgaaa taatgaatta
    14641 tcatcagtac tatcctgtcg acatcgtcaa cggccccggc actcgctgca ccctgtttgt
    14701 ctccgggtgt gttcatgaat gccccggttg ctataacaaa agcacatggc gggtaaattc
    14761 tggtcagcca tttaccaaag caatggaaga caagatcatt aaagatctga atgacactcg
    14821 tatcaaacgc caggggattt ccctctccgg cggcgatccg ctgcatccgc aaaacgtgcc
    14881 ggatattctg aaactggtaa aacgcatccg cgccgagtgt ccgggtaaag acatctgggt
    14941 gtggacaggc tataaactcg acgaactcaa cgctgcgcaa atgcaggttg ttgatttgat
    15001 taacgtgctg gtcgacggca aatttgtgca ggatttaaaa gacccttccc tgatctggcg
    15061 cggcagcagc aatcaggtgg tgcatcattt gcgatgataa gttccctgtt acctgagtgc
    15121 aggcaacagg gaaatgagga attaacgata cttctggtga taggcgttgc gggtcgtttt
    15181 cagttgctct gcagcagcct gcgcttcttt tactttacct tcatttgcca gcttcagcgc
    15241 gtcgtcaatc tgaccgacca gaatgtcgaa accgtggcgg aaatctttca tttccgggct
    15301 gtccggtgat ttatcttcga gcttcggcgg cgttgctttt tgcgcatcca gcgctgcggc
    15361 gcgcatcttc gttaacgcgt ctttgacttg cgtcgcgtta tcggcttttt cgaccacttt
    15421 taaattgtcg ttgagggttt ccatattgtc ttcaagatca gcggcaaacg acgccgaact
    15481 gaataccaac gaggagactg caagaatagc taacaggctt ttacgcattg ctcacttcct
    15541 tttttattta cacttcatcc ttcacactgc ctcatcgaca gctacgctca ttcgaccggg
    15601 tcacgtacta cgagtacgct ccccggctct cattcacttt ccgtctcgat gcagtgcgaa
    15661 tgattttgtg taatttttta gtttatagcg cggcaggtcg cgccagtttt tttactgtcc
    15721 ggcgattttc atctccggca acagcacaga accacactgt atattactgc gtgtttcaat
    15781 atcgttaccg acggtgacaa tattgcgcca catatctttt aaattacctg cgatggtgat
    15841 ttcgctcacc ggatactgaa tttcgccatt ctctacccag aagcccgcag caccacggga
    15901 ataatcaccg gtaatggcac tcacgccctg gcccatcagc tccgtcacca ccagcccggt
    15961 gcccatctct ttgagcattt gttcgaagct tagcccttgt ccggcaatcc gccagttgtg
    16021 aataccgccc gcatgtccgg tgcttttcag cccaagtttt cgcgccgaat agctggtcag
    16081 cagccactga gtcaggatgc catctttaat aatatcgcga cgctcggtgc gcacaccttc
    16141 gctgtcgaat ggcgtcgacg ccagcccttt cagcagatgc ggatgctctt caatggtcag
    16201 ccagtccggc agaatttgtt tacccagcga atccagcagg aaggtagatt tacgataaac
    16261 cgatccaccc gctatcgccc ccaccagatg cccaaaaagc ccagttgcca cttcattggc
    16321 aaaaatgact ggcgctttca tggtggagag tttacgcggt aacaggcgcg ataaagtacg
    16381 gcgagcacag tcggccccaa cccactctgg cgtttgcaga tcgctcatcg cgcgcccaat
    16441 ggtgtaggcg taatcacgct ccatatcgcc attttcttcg gcaattacac agctggagag
    16501 cgaatgacgc gttgagcagt aaccctgcaa catgccgtgg ctgttgccaa aaactttgac
    16561 accgtagtgg ctgttaaagc tgccaccttc ggtattggtg atacgtttat ccgcctgtaa
    16621 tgctgcctgt tctgcgcggg ccgccagttc aatggcttca tccggggaaa cttccgcagg
    16681 gtggaacaag tcgagatctg gtgcgtcaaa ggccagcagc tctttgtctg ccaggccggc
    16741 acagggatct ggcgaggtat aacgggcaat atccagcgcc gcctgtacag tacgggcaat
    16801 ggcctgcggg cttaaatcgg tggatgatgc gctacctttg cggttctggt gataaacagt
    16861 gatccccagc gcgccatcgc tattgaattc aacattctcc acttcaccat aacgcgtgct
    16921 tacgctaatg ccggtggtct tgctgacggc aacttccgca ccgtccgatt tgcctgaggc
    16981 caactccagc gcagtggaaa ctgcttcttc cagaatcttg cgctgcgctt caacttgaga
    17041 gattactttc attgcaagtg ccataatgta gagagtttct ctgaagtcta acagagaacc
    17101 gtttttcagt gcgcatctta actggtaaca ttagcctctt tttttaagga gcctgagatg
    17161 actaagcagc ccgaagactg gctcgacgac gttcccggtg atgacatcga agacgaagac
    17221 gatgaaatta tctgggtcag taaaagtgaa attaaacgtg atgccgagga gctaaaacgc
    17281 cttggcgcgg aaattgttga tctggggaaa aacgcgctgg ataagatccc gttagatgcg
    17341 gatctgcgcg cggctattga gctcgcccag cgtattaaga tggaaggtcg ccgccgccag
    17401 ttgcagctca tcggtaagat gctgcgccag cgcgacgtag agcctattcg tcaggcgctg
    17461 gataagctga aaaaccgtca caaccagcag gtggtgctgt tccataaact ggaaaacctg
    17521 cgcgatcgtc tgatcgatca aggtgatgac gccatcgctg aagtattgaa tctgtggccc
    17581 gatgccgatc gtcagcaact gcgtacactg atccgcaacg cgaagaaaga gaaagaaggg
    17641 aataagccgc cgaaatccgc acgccagatt ttccagtatc tgcgcgaact ggcggagaat
    17701 gaagggtaat tttcgttgtt gatgtgagta tggtttgccg gatgcggcgt aaacgcctta
    17761 tccggcaatg ctatatcagg ctgaagccga attactgcgc agcttccgcc ttcttcgcca
    17821 gaccatccag cagtttctga tggatcccac caaaaccgcc gttgctcatc accagaatat
    17881 ggtcgccagg ctgagcggtt ttcaccacca tatctgccag cgtatccaca tcgccactcc
    17941 agtgtgcagg ctgaacgcag gcttctgcca cttctgccac ctgccacgga atatgcgccg
    18001 gttgcagcag gaagacttca tcggcacgac ctaatgaagg tgccagatcg tctttgcaga
    18061 tccccatttt catggtattc gagcgcggtt ccagcacagc aataatgcgc gccgtaccac
    18121 caactttgcc acgcagcgcc gccagcgttg ccagaatcgc cgtcgggtga tgggcaaaat
    18181 cgtcatatac cgtgacgcca ttcgcttcac cacgcaactc cagacggcga cgagcattaa
    18241 taaacgaacc cagcgcgtta gcggcatctg ccgacgctac accaacatgg cgagccgctg
    18301 caatcgccat caggccattg tgcatattat gttcgcctac cagcgaccat ttcacttcgc
    18361 ccactttttc gccatccagc aaaacttccc attcggaagc atcggtggtc agctttttcg
    18421 cctgccagtg accctgctca cccaccagct cctgctcgct ccagcagccc atcgccatgg
    18481 tctgtttcag gttgatgtca ttttccggcc agataatacg gccctgcccc ggaacgatac
    18541 gcaccagatg gtggaactgt ttctggatcg ctttcaggtc gtcaaagata tcggcgtgat
    18601 cgaactcaag gttgttgagg atcagcgtac gcgggcagta atggacaaat ttagagcgtt
    18661 tgtcgaagaa ggcgcagtcg tactcatccg cttcgataac aaagaagtcg ctttcgccca
    18721 gacgcgccga aacctcaaag ttccccggca caccgccgat cacaaatccc ggtttgtaac
    18781 cacactgttc cagaatccag gtcgccattc ccgcggtggt ggttttgccg tgtgtaccgg
    18841 caacggccag cacccagcgg tcgcgcagca caaaatcgtg cagccactgt ggacctgaca
    18901 tataagggat gtttttttcc agtaccgctt ccacacacgg atttccacgg gtcatggcgt
    18961 tgccaataat caccagatcc ggctgcggct cgagctggct ggcatcgtaa ccctgaatca
    19021 gctcaatgcc ttgcttctca agtaaggtgc tcatcggcgg atacacattg gcgtccgaac
    19081 ccgttacttc atggcctaac tggcgcgcca gcatcgccag accgcccata aatgtgccac
    19141 aaattcctaa aatatgaatg cgcatacgtc actatccttt tttaatctgc cgctcatttt
    19201 acgcatatgt cgggcaagtg agaaacgcat ttcaggataa tccgcaattt gctggcgcga
    19261 ttcacctgag cgcaacattg agattatttg ttaagattgt tgcggtcgct ttactccata
    19321 aacattgcag ggaaagtttt atgaaaacgt taggtgaatt tattgtcgaa aagcagcacg
    19381 agttttctca tgctaccggt gagctcactg ctttgctgtc ggcaataaaa ctgggcgcca
    19441 agattatcca tcgcgatatc aacaaagcag gactggttga tatcctgggt gccagcggtg
    19501 ctgagaacgc gcagggcgag gttcagcaaa aactcgactt gttcgctaat gaaaaactga
    19561 aagccgcact gaaagcacgc gatatcgttg cggggattgc ctctgaagaa gaagatgaga
    19621 ttgtcgtctt tgaaggctgt gaatacgcaa aatacgtggt gctgatggac cccctggatg
    19681 gctcgtccaa catcgatgtt aacgtctctg tcggtaccat tttctccatc taccgccgcg
    19741 ttacgcctgt tggcacgccg gtaacggaag aagatttcct ccagcctggt aacaaacagg
    19801 ttgcggcagg ttacgtggta tacggctcct ctaccatgct ggtttacacc accggatgcg
    19861 gtgttcacgc ctttacttac gatccttcgc tgggcgtttt ctgcctgtgc caggaacgga
    19921 tgcgcttccc ggagaaaggc aaaacctact ccatcaacga aggaaactac attaagtttc
    19981 cgaacggggt gaagaagtac attaaattct gccaggaaga agataaatcc accaatcgcc
    20041 cgtatacctc gcgttatatc ggttcactgg ttgcggattt ccaccgtaac ctgctgaaag
    20101 gcggtattta tctctaccca agcaccgcca gccacccgga cggcaaactg cgtttgcttt
    20161 atgagtgcaa cccgatggca ttcctggcgg aacaagcggg cggtaaagcg agcgatggca
    20221 aagagcgtat tctggatatc atcccggaaa ccctgcacca gcgccgttcg ttctttgtcg
    20281 gcaacgacca tatggttgaa gatgtcgaac gctttatccg tgagttcccg gacgcgtaat
    20341 ctcctgcgcc tctccacacg gagaggcgtt tttattgctg cgcaatgttg actcttgtca
    20401 ctggcgaact ctgacgattc tcccacagca ccgttaatcc acgctgtaat gctataaaaa
    20461 taaacaacaa aataccgatg gcgattttcg tccaccagga actcagcgtg ccatcaaagt
    20521 ttatgtaagt ctgaatcagt ccctgaatcg ccacgccaaa aagcgtccct aataccgttc
    20581 caacgccacc gctcaaaagc gtaccgccaa ttaccactga ggcgatagca tccagctcca
    20641 cacctacgcc cgccagcgca tatccggcct gggtataaat cgagaagaca atccccgcca
    20701 gcgttgccag tccggtggag agcatataaa tgcgaatagt ggtgctgcga gtggaaatcc
    20761 ccatcagatt cgccgacgtt gcgttgccgc caatggcgta tacctgatta ccaaaacggg
    20821 tacgatgcgc gaggaatatg ccgataacca ccaccgccaa catcagcagt cccatcgcac
    20881 ttaagcgacc gccgccaggg attttccacg caaggcttga gagcgtgtca taaatgggat
    20941 ggtttatcgg aatcgactct tccgaaacga gatagctgac gccgcgcaaa aagaacatcc
    21001 ccgcaagcgt aatgatgaac gccgggatct tcagggcgtc gatcagaagc cccataaatg
    21061 cgccgaaggc acagcccatc accagcacca gcggaaacgc cagcagcggc gagaggccga
    21121 aatcgccaat cacttttgcc agaaacacgc cggtaaaggc gatcaccgaa ccgacggaga
    21181 gatcgatccc accggagagg atcacaaagg tcatgccaac ggcaatgatc ccaagaaagg
    21241 cgttatcggt caggatattg cagatcactc ttgtggaagc aaaaccagga aactgcgtca
    21301 ggcagtagag atagcccagc acaaagacgc cgatggtgat cataagcggc aagttacgtt
    21361 ttatcacggc tacgcactcc tttaatcaga ctgataaagc gttgcgactg aacaatcagc
    21421 acacaaagca ccaccaccgc tttcaccacc tggttcatct ctggcggaaa gcccgaaagc
    21481 aaaattccgg tattcattcc ctgaataatc agcgccccaa ctcccgaaag cagcaggtta
    21541 aagcgaccgc ccatcagcga tccaccgcca atcaccaccg cgagaatggc atccagctcc
    21601 agccataatc cggcgttgtt ggcatcggca ccgcgaatat ccgccgccac gataatgccc
    21661 gcaatcgccg cacatagccc gctcaacacg taggtgagca tgacgatgat tcgtgtgttg
    21721 actccggcat tttttgccgc ccgaatgttg ataccaacgg cttcgataaa catccccagc
    21781 gccgttttgc gggtcaacag ccagaacagg agaagcgtca gcaccgcgat aatgaccggc
    21841 gttggcagga acaacagcga tccgctgcca aaccatgaga gatccggcga gttaaacgtg
    21901 acgatctgcc cggcggtgat cagttgcgcc acgccgcgcc cggcgaccat caggatcagg
    21961 gtggcgacaa acggctgaat tttgaggatc gctaccagta tgccgttcca caatcccgct
    22021 aggatgccgg tgcccagggc gcttaacaaa acaatcggca ggctgaatcc cgcgaccgtc
    22081 atcgcagccg ttgtggctcc ggcgatagcc atcaccgccc ccacagagag atcgatacca
    22141 ccggtggcga tcaccagcgt cataccaatc gccagtaacg caacgggagc tgcacggtta
    22201 agaatgtcga tagggctacc gaacaaacgc ccatcctgaa gcaccacctg ccagaaatgc
    22261 ggggctacca ggctatcgac cagcagcacc agcaacagtg ccgccagctg cggcatcccc
    22321 gtcggccagt gaaaacgcct ttttggcggc gtagtgtcag ggagagattg aggcatcaca
    22381 ctgttctcct tacgccgcaa tggcgttcat gatcgccgga acggaaagct gtgccagcgg
    22441 gatctccgcc acctgtttgc gatcgcgcat gatgatcacc cggtcggcat agcccaccag
    22501 ctcttccagt tcggaggaga tcaccagcag cgccagaccg tcagcacaca gcgtttcaat
    22561 caggcggatg atctcggcgt gggcaccaac atcaatgcca cgggttggct catcaaggat
    22621 cagaaattgc ggtcgggtca gtagccaacg tgaaagcagc actttttgct gattgccacc
    22681 ggagagaaat tcaatcggtt gttcagttga aggtgtgcga atgccaagct ggcggataaa
    22741 gcgttcggca atctcttgct gctctttgcg ggaaatgggt cgtagccagc cgcgctgggc
    22801 ctggagagcg aggatgatat tttcccgcac cgaggcggcg gcgatgatgc catcggtttt
    22861 cctgtcttcc gggcagaatc cgatgcccag tactgaagcc tgatgtggcg atcgcagggt
    22921 ttgcggtttg cctttgatca acgccgtgcc gctgtcagca ggtttgatac cgaagatcac
    22981 ttcggcggtt tcggtacgtc cggatcccag caagccagcc agaccgacga tctcgccggg
    23041 gcgcacttcg agatcaaacg gtgctatcgt tccttttttg ccgtaatttt tgaacgcggc
    23101 aacgggtttg tcgctcaaca atgttcgccc ggcacgctgt agcacgtggg tatccagctc
    23161 gcgccccagc atcattttta ccagttcgat ctgcggtagc tcgcgcgttt cccgacagcc
    23221 tacgaaactg ccgttgcgta agacagtgat ccgatcgctg acctgataga cctgatcgag
    23281 aaagtgagtg acaaagatca ggctgacgcc gcgatcgcgc aactgacgca tcaggccaaa
    23341 taatagctcc acttcctggg tgtcgaggct ggcggtgggt tcatcgagga tcagcacttt
    23401 ggcggagaga tcgatagccc ggcaaatggc gacgatttgc tgcatcgcga ctgaaaagcg
    23461 gttaagcggt tcgcgcacgt cgaggaagaa accgtaagat gccatcagtt cggtggcgcg
    23521 cttttccatc tctttacggc gtagaaggcc gaaacgtttg ggttcgcgac ctatgaatag
    23581 attatcagca accgacatat tgggtagcag gttgacttcc tgatagacgg tgccaatacc
    23641 gagttgttgt gcatgggcgg tatttttcgg tgagatagcc tggccttcca gccagatggt
    23701 gccgcgatcg gcgtggtata caccagttaa tgctttgatt agcgttgatt ttcccgcccc
    23761 gttttcacca agcagcgcca tgatttcgcc acggcgcagg ctgaaatcaa cgttgtctaa
    23821 cgctttgacg ccggggaaaa atttacttaa tccttcggtg cggaggatct cctggtgttg
    23881 gtcggtcgtc atggtttccc ccggtgtaaa gcgtgctccc agtaggcatg ataagacgcg
    23941 gtaagcgtcg catcaggctt cagtgcacca tcgccggatg cggcgcgagc gccttatccg
    24001 gcctacgaat cggcatcagg catagcaacc ctcaataccc catatttttc ttcttctcta
    24061 actcttcttt tgcggtatca ggcaggtaaa gggtggattt ggttaacgtc agcttttcag
    24121 gcatggtgcc gtcttttttg tatttctcca gcgcatcgaa agcgggtccc gccatgttcg
    24181 gcgtcagttc aacactggcg ttcgcttcgc catccatcat cgctttgtag atgtccggta
    24241 caccgtcaat ggaacccgtg aggatatctt tgcccggttt caggcccgct tctttaattg
    24301 cctgaattgc accaatcacc atgtcgtcgt tatgggcgta aaccatgcag atgtttttgc
    24361 cgttgttttc cgctttgata aagctctcca tgacttcttt gcctttactg cgggtgaagt
    24421 cacctgactg cgagcggata attttgatat ttggcgcatt cttaatggct tcggcaaagc
    24481 ctttcttacg gtcaatggcg acgctggccc caacggtgcc ctgcagctcc accacgttgc
    24541 atggtttgcc attcacttct tttaccagcc agtcaccgat caacttacct tcgaggatgt
    24601 tgtcggcagt aacggtggtc atatagagag atttgtcttt cacatcgatg gaacggtcga
    24661 gcaagaagac cgggatttcg gcatctttcg cctcttttaa taccggttcc caacccgtcg
    24721 caaccactgg agcgataaag atcgcatcca ccccttgtgc aacgaaggaa cgtaccgctt
    24781 taatctggtt ttcctgcttt tgctgaccat cggcaatttt caacgtaatt ccgcgttttt
    24841 cggcctcact tttcgccaca ttggtttctg cggcgcgcca gccggattcc gatccgacct
    24901 gcgaaaatcc tccggttaat ggagctgcca acgccataga cgacatggct gccgagactg
    24961 cagagactac aagtaagcgt ttccacatag ggttgtcctc gtcggggtaa ttattattgg
    25021 tgaaaagatg ttcgcgaaaa aactatagac aattcgttat gtaacggatt gcgttacatc
    25081 acacttcaaa aaagtaaata aaacgttaat cacaagtttg taatcgcttt catctcacta
    25141 tgaaaaatgc ggctacggtt atggattttc ctgctctgta taccgtctta aaactggcga
    25201 aaaaggaaaa tgaagacgaa aacaagcaaa gacattcggc gcgagttggc tataatactc
    25261 ggcacttgtt tgccacatat ttttaaagga aacagacatg agcttactca acgtccctgc
    25321 gggtaaagat ctgccggaag acatctacgt tgttattgag atcccggcta acgcagatcc
    25381 gatcaaatac gaaatcgaca aagagagcgg cgcactgttc gttgaccgct tcatgtccac
    25441 cgcgatgttc tatccgtgca actacggtta catcaaccac accctgtctc tggacggtga
    25501 cccggttgac gtactggtcc cgactccgta cccgctgcag cctggttctg tgatccgttg
    25561 ccgtccggtt ggcgttctga aaatgaccga cgaagccggt gaagatgcga aactggttgc
    25621 ggttccgcac agcaagctga gcaaagaata cgatcacatt aaagacgtaa acgatctgcc
    25681 tgaactgctg aaagcgcaaa tcgctcactt cttcgagcac tacaaagacc tcgaaaaagg
    25741 caagtgggtg aaagttgaag gttgggaaaa cgcagaagcc gctaaagctg aaatcgttgc
    25801 ttccttcgag cgcgcaaaga ataaataagt tcttctagcg caataaccct gaacgccggg
    25861 cttcggttag taagggtttt ttatgcccgc gataaataaa ctctctattc caccatcatt
    25921 attctcagcg gttgcaaggc ttgaacggta agaacaagca aacccgacca ccattttgct
    25981 gttcatagcc acttgctgga agttagccga cctcactcat actcaccgtt caaagcgtgc
    26041 ccccgtttta cccctgcaca tttgtgaacg ccagcagagt tttttatgcg attgcagata
    26101 tggaaagcgg ctcggagaga aaagcaaaag atggaaaagt tacaaaaaaa aatcgcgaga
    26161 tacatcgcat aaaacaacgc cacccgaagg tggcgtgctt ttattattac ttatccctgt
    26221 ctaaccagtc gccgctttca attagcttta atccatcgac gggtcgttga taaacgtaca
    26281 tccatgcact cccgtacggc gtctgaatca actggcgcgc gtattcaccg cccctggtgc
    26341 gcaaggcatc aagttcggcc agcgtggcgt tgtcaatacg ataaacttca ccgtgtaccg
    26401 ttccgttccc cggaactgcg cctggatagt ggcccaggct atacaactgg tagttatcga
    26461 tactgaaatc gcccagtaac tgggcattgg tcatccagtg actgttgcct tgtttgtggc
    26521 gtaaactgcc gtagacaaat attcgcattg ctaaaactcg aactgataga gcaaatccag
    26581 tgcctggtct acaccagaca cggcttccag atatagctta ggcatcaggc gataacgtaa
    26641 cgtgagtgtt gctatagagt caaatatacc cacgccgtat ttcacttgca gacctggcaa
    26701 tacatagccg ctgaccacta cctgggagga gtcgcctact ccctgggtgt cgagcgctaa
    26761 attgcttacg ccaaacgtct cgccgatttt acccacaatc tggccacttt gcgcaacccc
    26821 cagaccaatc agcatcgagg tcattgccgc actgtcgctc tgatcgctct ccagcccttg
    26881 tccacgtagc aaataagaca atgcagcttg ttgcgacatc gccgggtcag agaagatctc
    26941 cgctttcggt tcgtccgcca gaccagtgac gcgaactccg gcaattacgt cgtcttctgt
    27001 agcatccggg ttacgaatag cttcaatatt aagatacggt tgatccggcg gaccagagaa
    27061 cagtaactca cccttacgca caatcagatc ctgaccatag gcatggaagc gcccttcagg
    27121 gatgttgatc tgcccgttca ggcccagccc ttgtttgtcc tgtacgacgt taagatcgcc
    27181 cgtcagccgc gctttcaggc caaaggcgtc aatgcgcaca ttgttgccaa cgtggacaat
    27241 caggttactg ttaatcggaa tcgacgccgt tttcggctct tccggttgca ggttatcgtt
    27301 aagcatcacc acatcgctgg agacgcctac tgcgctttcc ggcagatcgt gcaccacgat
    27361 gcgcgcccac gggacatcca cgcgaccatc gagggtaaac aggtttggtg tagcctcgaa
    27421 tacaacatct ggcgatacat ccattcgtac catcggcggc acggtgatcc gcactttact
    27481 gcctttcgcc gttactcgtg cccgccagtt ttcaatttgg ctccagtcgg catcaccgtt
    27541 caggtagatt tcaccctgct gggtccgtac tgtaccggca agcgtcgagc gcataccgtt
    27601 aaagttgacc gcaagctggc tcggctgcat atcaaacggc ataaagttgc cgtcgatatc
    27661 cacacccgta acctgaagct gaccaaacaa ctgcgggctt tgcacatcac cacccagacg
    27721 caagttggca ctcaccatcc ccgctgcttt ttccccacgg gtaaagatgg ggtttatcat
    27781 cgcaaggttg aagttacgga tattgacgtt gccaccaaga ttacggcggc cttgcggatc
    27841 ggtcacctgc acctgtccat caaactggcc gttattggtc aggcggatgg tccagcccaa
    27901 ttcggcacgg ttgttacgca attccgccgt cagattcagt gtctgaaacg ccaccggcag
    27961 cgccgcatcg ttgacggttt gcgttacctg cacgttacgc cccgaaaggg tgatactgcc
    28021 ctgcggcagc ccctctttcg tggtgtccca ggcaacatcc gctttacccg tgaagatacc
    28081 gctggcctga gtggtttctg gcataaacgg tttcagcatg gcgaggtcga agcggttgag
    28141 attcaccacc gcacgccctt cggcccccgc atcgatagtt tgcggcacgc acagttccgc
    28201 attcgggtta agccagcagt gtggcccgat gctgattttt tgctccttat tgcggtaatc
    28261 cagcgcaata tcgcgggtca gcgaccacgg gccgaccggc gtctggaaac gggtattgct
    28321 aagagttccc ttccagcgtt cttctttgcg atcaaaactt cctgccagat taagctgccc
    28381 ggagacaggc tcgccctgaa tccgcaactg tagctcgtgc tgcttttcgc tgcctttggc
    28441 attcagggtg acgaggttga tatttacatc cggctgcgaa atttgctcaa cgcgtacgtc
    28501 gagtttcccg gcgatctgat cggtggattt aatgtcgcct tccacgcgaa cctgcgccac
    28561 ggaaagttcc tgccagcgca ggccgcgcgc ggtgatatct gccagtagtt gtggcgcttc
    28621 caccgtgccg cgtactttca ccagcccttt cgctgtaccg ccaagccccg gcagcgcgtt
    28681 atccagcccc ggcgcgttga tggtggcatc aagattgaga tcttttaccc ccagctcgcc
    28741 tttcacttcg gcactgtttg gcccgagttc cagatgaagc ccagggatca tccactgcat
    28801 ataactgttg cctttcagcg tgccgtcaac gttcactttg ttctgtttga cgttaccggt
    28861 cagcttcagt tctggcacct ccatctgcca ggtgccgccg tacaggctac cgcgggtttt
    28921 aatcaagcca ttgagtttcg acggccactc cgggaactct ttggcggtgt taatgccatt
    28981 aagcgttagc tcaccgcgcc aactaatggc ctgctgccag tcgagcaacg ccttgagttc
    29041 agttttccct tccagcgccg cgacggtgag tttgtcgaga ttgacctgct gttcattacc
    29101 tttggcatca agggtaatgg tggctggcgg gatctcctgt cccttcactg ccgtacgcat
    29161 agagagcgtg taatcggtca ttttgccggt aagtttcagt ttcagatcat ccgcctgata
    29221 ctgcttctca ccagtgaacg gccagtaaag ctgtttgctg ttcacttcca cgttgagtgg
    29281 caatccggct tccgccagtc gcgtctgggc gcgtaaatcc atatccaccg gaccggaaag
    29341 gttaacgcct atctccagct gttcgcgcag cgcgccgccc actttcagct tcaccttttc
    29401 acctttcaac ggctccacgt tcagtgtgct gttgaggatg atatccaccg gccagttgtc
    29461 tgacagctgc gccgtgccgc tggcgttgac gatcccctgg ttggaatcga tatccagggc
    29521 gtccagttta gtattgccgt caatgctgct cactttcagc agcatggtgc gcacggtgat
    29581 gtccgtgtcg cccgtcacgc gcagctgctc gcccttaaac tcttcaatgt tcaggttaag
    29641 cggcaaatgc acgtcggtca tttccggcaa taccgggcga gaaaagagat ctttcagcgt
    29701 ttcgccgagc ggcttttcat ccggctgcgg attttcaatc ttcggttcga cgacctcttc
    29761 ctgcgccact tccgccactt tcggcagagc aatcagcagg cctttcagcg acgtcggttt
    29821 cagggtcagg gttttctcct gccagttcag gccggaggtg aagtccatca ccgacaccgt
    29881 ggtgtcatca atcttgatgt tgacgttatc cagcgccacc cgtgtcaggg tgatgggata
    29941 cggcgtggag agatccagcg gaccgctatc ttcctcttct tcaacctgtt cagaaggagg
    30001 cattttttta ctgtcgatgt tgacctgaat gtctttcagc gccaggtcat taatacaaac
    30061 gctactgttc cacaggcact caagaccgac cgccagatgc agattacccg cttttaccgc
    30121 cacgcctggc tgctcataac gaacgtcaga caaggtgaga tcgcgccagc cgccggtgac
    30181 gttgccaata tccagtcctg gcacccagcg atccgccgct ttaaatacaa gatgcaggcc
    30241 gctggtggtg cccaccagaa acgccaccga tcccagcaac agtaagataa cgataaccac
    30301 gccgaggctg atttttttcc ataaactcat aattctggcc ccagaccgat gtaaaactgt
    30361 aacccgtgtt cgtctttatc cgcgaccggt acggcaaaat cgagtttgat tggcccgacc
    30421 ggcgattccc agcgtacgcc gcccccggta ccggttttaa agtcgctgcg gcgaatatcg
    30481 cttaccgctt cgccactatc gacaaacacc gcgccccacc attttccggt cacgttgtac
    30541 tggtattcca gcgatccggt tatcagcttc gaggcccctt tcaggtcacc gttggcgtat
    30601 ttcggggcga tagatttata tttgtagccg cgaatactac gatcgcctcc ggcgaagaaa
    30661 cgcagatccg gcggtacttt gtcgaaatca ccggtttcaa tccagcccag cgtgccgcgt
    30721 gtcacaaaac gatggcgatc gtacagtgtg cggatccaga cgttctgcgc ctggaaaacg
    30781 gagaaatcaa catctgaacc ccaggccgtg ttggagtagt cgatagagta gcgttgcgag
    30841 tcgccccagg ttggcatcag gccaccacga gaacgcgtgc ggctaatcat caccccagga
    30901 taaaacagca tcgtggtgtt ggtaatttca ccctgggtaa agtggtcgag gctccagcgc
    30961 aggttaatgg cacgctgcca gtcgctggag agatcccagt agcgagaagc caccagcgta
    31021 gtggagtcag attcggtatc gttcaggtca gtgcgcttaa aaccgccctg caccaaataa
    31081 tattgttcca gtggattctt cagcagcggc attttatagc tgaagtcgag gatctgttcc
    31141 ggcgcggaaa tactggtact ggtggtcaga ctgtgaccat aagagttcat ccacggcttt
    31201 ttccacgtcg ctttcacgcg cggtcccacg tccgtagagt aaccgacccc ggtttcgatg
    31261 gtgttttctg ttcgcggcga aaccacgccc gtcaatggta atactttcgt ttcgcgcgct
    31321 ttatcaaatt gcggtgccac taccaccgaa ttaaaccagc cggtagcaga aagtcggcgg
    31381 ttcagttccg ccagatcttt cgattcatac tcatcgccct ctttaaacgg caccagattt
    31441 tgcaggtatt catcgcggat ttgtgatcct tcaaaggtca catgcccaaa gcggtaacgt
    31501 tcgccactgt tataatcaat atcccagaag gctttatgca ggccgagcgc aatgcccagc
    31561 tgcgctttgg taaattcgct atcgaaataa cctttacgca acgcaatact ggttaaggac
    31621 tttttgaaat tttcataatc gccctggttc agtaccgtac caatagccgg gcgagtatcg
    31681 agcaatttca aatagtcttt atcggtccgc gcgccgccgc gcaataccac atcggtgccg
    31741 ccaattaaca ccggtacacc tggcgtgact ttggcgatca atacctgccg ccctttcttt
    31801 ggcggtggac ggagatcaaa ttcaatggtc ggctggtaat aacccagcgc tttcagacct
    31861 tcacggatgg catcatcgac gcgtgcgcga aagcgacggt ctggcgtcac ttcatcactt
    31921 tcaatcgtag aaagctgcgc acgaacgttc ttttccagct gtcccgataa cccctcgacc
    31981 tgtagacgga cgttcgcggc gacggcagat ccgcttaagc agagtaagct tacacagcat
    32041 aactgtcgga tatagcgcac attttctcct gaatatcctt ttttcctacc cctggaacgc
    32101 cgttaaacgg cttaacaaaa atccagtaat atggattaaa aagcagacta aaccccaaat
    32161 atttcttatg ttttacttta gacctattca cggtggttat tgtgtgcaaa tacgcctctt
    32221 gttacaacct taaccccaat gaccgatttt cgggagagcg acaccatgag tttatttgat
    32281 aaaaagcatc tggtttcccc cgccgatgcc ctgcctggac gtaacacccc gatgcccgta
    32341 gccacgctgc atgcggtcaa cggtcactca atgaccaatg tacctgacgg aatggagatt
    32401 gccatttttg cgatgggttg tttctggggt gtggagcgtc tgttctggca gttacccggc
    32461 gtttacagca ccgccgcagg ctataccggc ggctatacgc caaatccgac ttatcgggaa
    32521 gtgtgctccg gtgatacggg tcatgccgaa gcggtacgca ttgtttacga tccttccgtc
    32581 atcagctatg agcagttgct acaggtattt tgggagaatc acgatcccgc ccagggaatg
    32641 cgtcagggca atgaccacgg cacgcagtat cgttcagcga tttatccgct gacccctgaa
    32701 caggatgccg cagctcgcgc cagtctggaa cgttttcagg cggcgatgct tgccgccgat
    32761 gacgatcgtc acatcaccac ggaaatcgct aacgccacac cgttttatta tgccgaagat
    32821 gaccaccagc aatatctgca taaaaaccca tatggttact gtggaattgg cggaattggc
    32881 gtctgtctgc cgccggaagc atagcgttac gggtacaaat gtagtttgtt gataaagtgc
    32941 tctttgtcta tgcctgatgc ggcttaaacg acttaccagg cctacaaaat cgtccaaatt
    33001 caatatattg caaggactgc gtaggcctga tcggcatagc gcatcaggca gtattgcatt
    33061 tatcagcagt ctgaatttta accctctggc gactttacag taccttacgc tatactagcc
    33121 actgaaaatg tcggttcact ttcttcgaat cggctttcaa tgtgtatttc acacaaatta
    33181 atcaactttc ccttccgagg atctggcctg aaaggtcgga taagatatgt taaacagtat
    33241 tttagtcata ctctgcttga tcgctgtaag tgcgttcttc tcgatgtccg agatctcgct
    33301 tgccgcctca cgcaaaatca aacttaaact gctggctgat gaaggcaata taaatgccca
    33361 acgcgttctg aatatgcagg aaaatcccgg catgttcttt accgtggtcc aaatcggtct
    33421 gaacgcagtg gcgattctcg gcggtatcgt cggtgatgcg gcattttctc cagcttttca
    33481 cagcctgttc tcccgctata tgtcggcaga gctctctgag caactgagct ttattctctc
    33541 tttctcgtta gtgactggca tgtttatcct gtttgcggat ttaaccccga aacgcatcgg
    33601 tatgattgcg ccagaagcgg tggctttgcg tatcatcaac ccgatgcgct tctgcctgta
    33661 cgtttgcacc ccactggtgt ggttcttcaa cggcctggcg aacatgatct tccgtatttt
    33721 caaactgcca atggtacgta aagatgacat cacttctgat gacatctacg cggtagtgga
    33781 agccggtgcg ctggcgggcg tgttacgtaa acaggaacac gagctgattg aaaacgtctt
    33841 tgagctggaa tcccgtaccg ttccgtcttc aatgacaccg cgtgaaaacg tgatttggtt
    33901 tgatctccac gaagatgagc aaagcctgaa gaataaggtg gcggaacatc cgcactctaa
    33961 gttcctcgtc tgtaatgaag atattgacca catcatcggt tatgtcgatt ctaaagacct
    34021 gctgaaccgc gtgctggcta accaaagcct ggcactgaac agcggcgtac aaattcgcaa
    34081 cacgctgatt gtgccggata cgttaaccct ttcagaggcg ttggaaagtt ttaaaaccgc
    34141 aggtgaagac ttcgcggtga tcatgaacga gtacgcgctg gtggtgggga tcatcaccct
    34201 caacgacgtg atgaccacgc tgatgggcga tctggtcggt caggggctgg aagagcagat
    34261 tgtcgcccgt gatgagaact catggctgat tgacggcggc actccgattg acgacgtcat
    34321 gcgcgtgctg gatattgacg aattcccgca gtcgggcaac tacgaaacca tcggcggctt
    34381 tatgatgttt atgctgcgta agatcccgaa acgcaccgat tcggtgaaat tcgccggcta
    34441 caaatttgaa gtggtggata tcgataacta ccgcatcgac cagctgctgg tgacccggat
    34501 cgacagcaag gccaccgccc tttcgccaaa actgcctgac gctaaagata aagaagaaag
    34561 cgtcgcgtaa ccccccagaa acatcaacgg ctcctgaatc aggagccgtc ttattactgc
    34621 atagcacttt ttactgcata gcactttggt taagccatct ctgtttgcag acgcataacc
    34681 tgacggttaa cttcggacat cactgacaaa tgcagcttat ccttcacttt tgggataaga
    34741 atcttaccct tatcaaattc aaaagcgcca acgtccttga tgtataaccg tccacggaac
    34801 aggatcttta cgtacttcgc cacctgaagt gggttgtacc gttggaaaat tttcattgtt
    34861 atctcctgct gagtattacg cctgtgcggt gccacaatcg gcccaactat tatgaggcgc
    34921 aaattttaat gcttagtgac tatagactat ccgggcaatg tttccaccgc gtataacttt
    34981 ttttacctaa aggttacaat tattcagaat tatcttttta ccgaagcgcg gtcttcagta
    35041 taagcattca tttttcatat gatttgtgcg cttgaccgca aactggcatc acacttgcgg
    35101 gaaattcgat aaatagcaca tatgattaaa actcagaccc aagtggtcgg atcacctgca
    35161 tatcataaga aggaaacacc atgaccctac gcaagattct ggcactcacc tgcctgctgt
    35221 tgccgatgat ggcttccgca catcagttcg aaaccggtca gcgagtgccg ccgattggca
    35281 ttaccgatcg gggcgagttg gtgcttgata aagatcagtt tagctacaaa acctggaaca
    35341 gcgcgcagtt agtgggaaaa gtgcgagtac tgcaacatat tgctggtcgc acctctgcaa
    35401 aagagaaaaa cgcgacgctg attgaagcga ttaaatcagc gaagttaccg cacgatcgtt
    35461 accagaccac cactattgtt aacaccgacg acgcgattcc gggttcaggg atgtttgtgc
    35521 gcagcagtct ggagagtaat aaaaagcttt atccctggtc gcagtttatt gtcgatagca
    35581 atggcgtcgc gcgcggtgcc tggcagctgg atgaagaaag ttccgctgta gtggtgctgg
    35641 ataaggatgg tcgcgtgcaa tgggccaaag acggggcgct cactcaggaa gaggtgcagc
    35701 aagtgatgga cctgctgcaa aagttgctca aataatggca acaacataga ttctgcaaaa
    35761 ataattttcc cctatcgtta tctatgggat aggggaatta agtctctgag ttcttacgcc
    35821 gaactcatac tggtaacgct agttgctctt ttttaaacgc ctcaatggtc gacttatatt
    35881 tcttgcaaat cttttctttg gtaatattac tatctgcggc cagattcgca cttactatcg
    35941 catccacttg ttcggctaat tccacttgtt ggcaacctgc attaaccaga aaattataaa
    36001 taagggagta atcttcatcc tccaaaacaa cagaggaacc attacgaaaa cgttcatccc
    36061 ctccatagat tttaagcgca aagcccaaag attctatgac tatggcaaaa ttatcatcaa
    36121 ttattttttc gtgaatatac catccatcgc attctttaat tccacctcct ggtatttgga
    36181 gttcaaattt accccgtttt aatttgctta ctatatcctc caataaggca taacctaaag
    36241 acacgtcacc aatcgcctgg tgaatataac ctttaatcct tttcggtatg tcagactgtt
    36301 ttttctgcag aatatcacag gcatacaatc ttctgttttg ttctaacgga aactcgcggt
    36361 ttttgaaatc catgaatgag ttatactcat ttattgttat gggaatttct tcgtattcaa
    36421 atccactacc tcgcaatgtc attgtatatt cggcaccatt atctttaagg ctgaaactga
    36481 taaggaactt atccatggcg ttaacagttc cattttttga tgaaagtacc tgataactat
    36541 ttttatcaat ctgagttgcg caacgaaaga actccctcaa agttaacagg ctatcataat
    36601 tcgaaatcca atcttttttc ttaaatgcat gctggatatt gttaaaaaaa ttgtttgaat
    36661 gtgtttttgt atacgatgac tttacaaata actccaccgc accaataaaa ttattgtgtc
    36721 tgatcctggg taacatatga caaccttatt ttatttggtt attttatgtc gccattaaat
    36781 atctcattag agaagtaaac tatcaaaaaa cgctcattat taaaatgtaa cttattagca
    36841 attgaatttt tgcactttaa atacaatctc tttttattta taacttttta ggttgggaga
    36901 aatcaggatg gcagaatcag gaaatacact gtttctgcca tctgaattta gtaaatagac
    36961 actctgaacc ccggattcag gaacgactcg cgcggagtgt aatccagcgg tttaccctgc
    37021 cagtcgtgaa cgtgcgctcc ggcagctgca gccacagcat gtccagcggc ggtgtcccaa
    37081 atattcgttg gtccgaagcg cgggtacagc tgcgcctgtc cttccgccac caggcagaat
    37141 ttcagcgaag agccgatgga cgtggtctga tgttcgccaa gctgttgcag atactctttc
    37201 agctccgcat ctgcatggga acggctgata accaccagcg gcgggcgcgc atcgcggacc
    37261 tgaatctgct tgcgcacacc gcactcttct ttccaggcct tgccttctgc cgcgctgtac
    37321 attacgttca ttaccggcgc atacaccaca cctaaaatcg gtttgccatg gtcaatgagc
    37381 gcaatgttaa cggtgaattc gccattacgt ttaataaact ctttagtacc atccagcggg
    37441 tctaccagcc agtaacgctg ccagtgctga cggacttccc aaccgggagg atcttcttca
    37501 gaaaggaccg gaatatccgg tgtcagcgta cgtaaaccgt ccatgataac ggtgtgtgcg
    37561 gcaatatccg ctgccgttac cggagaattg tccgctttgc tgacgacgtc catcggtttc
    37621 gtcccgtcgt agacctgcat aatggcatcg cctgcattcc gtgcaagctg gcatacttga
    37681 tctaacattt ctccacctcg tctctgtgag cggtgttaac ttattgtttt acttataccc
    37741 tatcgttaat gaatgcgcca actgtgatag tgtcatcatt ttcaaagcgt aaaattgtgg
    37801 cattcttcac tgttctataa gtaagacgtt tattctccct tttctttcgt attcccgatg
    37861 ataaaaggat gtccctgatg attaagttta gcgcaacgct cctggccacg ctgattgccg
    37921 ccagtgtgaa tgcagcgacg gtcgatctgc gtatcatgga aaccactgat ctgcatagca
    37981 acatgatgga tttcgattat tacaaagaca ccgccacgga aaaattcgga ctggtacgta
    38041 cggcaagcct gattaacgat gcccgcaatg aagtgaaaaa cagcgtactg gtcgataacg
    38101 gcgatttgat tcaggggagt ccgctggcag attacatgtc ggcgaaagga ttaaaaacag
    38161 gtgatgttca tccggtttat aaggcgctga atacgctgga ttatacggtc ggtacactcg
    38221 gcaatcatga atttaactac ggtctggatt acctgaaaaa tgcgctggcg ggagcgaaat
    38281 tcccttatgt aaatgccaac gtcattgacg ccagaaccaa acagccaatg tttacaccgt
    38341 atttaattaa agataccgaa gtggtcgata aagacggaaa aaaacagacg ctgaagattg
    38401 gctatattgg cgtcgtgccg ccgcaaatca tgggctggga taaagctaat ttatccggga
    38461 aagtgacggt gaatgatatt accgaaaccg tgcgcaaata cgtgcctgaa atgcgcgaga
    38521 aaggtgccga tgtcgttgtc gttctggcac attccgggct gtctgccgat ccgtataaag
    38581 tgatggcgga aaactcagtt tattacctca gtgaaattcc gggcgttaac gccattatgt
    38641 ttggtcatgc tcacgccgtt ttcccgggta aagattttgc tgatatcgaa ggggctgata
    38701 tcaccaaagg cacgctgaat ggtgttccgg cggtaatgcc gggcatgtgg ggcgatcatc
    38761 ttggggtggt cgacttacaa ctcagtaatg acagcggtaa atggcaggtg acgcaggcga
    38821 aagcggaagc tcggccgatt tacgacatcg ccaataaaaa atccctcgcg gcggaagaca
    38881 gcaagctggt agaaacactc aaagccgatc acgatgccac acgccagttc gtcagcaagc
    38941 caatcggtaa atctgccgac aatatgtata gctatctggc gctggtgcag gacgatccga
    39001 ccgtgcaagt cgtgaacaac gcgcaaaaag cgtatgtcga acattacatt cagggcgatc
    39061 cggatctggc aaaactgccg gtgctttcag ctgccgcacc gtttaaagtt ggtggtcgca
    39121 aaaatgaccc ggcaagctat gtggaggtgg aaaaaggcca gttgaccttc cgtaatgccg
    39181 ccgatcttta tctctacccc aatacgctga ttgtagtgaa agccagcggt aaagaggtga
    39241 aagagtggct ggagtgctcc gccggacagt ttaaccagat tgatcctaac agcacgaaac
    39301 cacagtcact catcaactgg gatggtttcc gcacttataa ctttgatgtt attgatggtg
    39361 tgaattatca gattgatgtt acccagcccg cccgttatga cggcgagtgc cagatgatta
    39421 ataccaatgc ggaaaggatt aagaacctga cctttaatgg caagccgatt gatccgaacg
    39481 ccatgttcct cgttgccacc aataactatc gcgcttacgg cggcaaattt gccggtacgg
    39541 gcgacagcca tatcgctttt gcttcaccgg atgagaaccg ctcggtgctg gcagcgtgga
    39601 ttgctgatga gtcgaaacgt gcgggggaaa ttcacccggc ggcagataac aactggcgtt
    39661 tagcaccgat agctgccgat aagaaactgg atatccgttt cgaaacttcc ccgtcagata
    39721 aagccgcagc gtttattaaa gagaaagggc agtatccgat gaataaagtc gcgaccgatg
    39781 atatcgggtt tgcgatttat caggtggatt tgagtaagta aaacacttct ttttcggcct
    39841 ataaatcatc aatcgcatcc ggcatttatt ggcggatgcg atgctggcgc atcttatccg
    39901 ccctacaagt catacaccgt aggccgcgca tccggcagtt atgccgcacg ttcatcccgc
    39961 accgccaaca cctcgggcaa attcaactca atccagtccg ccagtgcagc aaccttttcg
    40021 ctcacctgct cgcccagcgg cgtgaggctg tattcaacat gcggtggcac caccggatac
    40081 gcgatacggt taagaaaacc atcctgttct aacgcctgta acgactgcgc aagcatcttt
    40141 tcgctcaccc cgccgatttt gcgccgcagg tcgctaaagc gatgagtacc ttcgcgtagc
    40201 gccaccagaa tcaacacccc ccaacggctg gtgacgtgtt tcaacacctc gcgcgacggg
    40261 cactgttccg caaagagatt accctctttc agttgttgcg acaggctaac ctgactcatt
    40321 tcatacttac ctttttgtac gtacttacta aaagtaagtt taggtgttag cgtatttaaa
    40381 cacaagacaa aacgatggag acttcccatg atcgctatta ctggtgccac tggccaactt
    40441 ggtcactata ttattgaatc cttgatgaaa acggttcctg ccagccaaat agtggctatc
    40501 gttcgtaatc cggcaaaagc ccaggctcta gcagcacaag gcattaccgt gcgtcaggct
    40561 gactacggcg atgaagccgc actgacatct gcacttcagg gagtggaaaa actactgctg
    40621 atctcttcca gcgaagtggg tcaacgtgcc ccgcagcatc gtaatgttat taatgccgca
    40681 aaggcggctg gcgtgaaatt tatcgcttat accagcctgc tacatgcaga tacctccccg
    40741 ctcggcctcg ccgatgagca cattgagacg gagaaaatgc tggctgattc tggcatcgtt
    40801 tacaccctgc tgcgcaacgg ctggtacagc gaaaactacc tcgccagcgc cccggcagca
    40861 ctggaacacg gcgtatttat cggtgcggcg ggcgatggca aaatcgcctc agcaacgcgg
    40921 gcagattatg cggcagctgc ggcacgcgtg attagcgaag ccggtcacga aggcaaggtt
    40981 tacgaactgg cgggcgatag tgcctggacg ttgacacagt tagcggcaga gctgaccaaa
    41041 cagagcggca aacaggttac ctatcaaaat ctgagcgaag ccgatttcgc cgcggcactg
    41101 aaaagcgtcg gactgcccga cggactggcg gatatgctgg cggattctga cgttggcgca
    41161 tcgaaaggtg gtctgtttga taacagcaaa acgcttagca aattgattgg ccgcccaacg
    41221 acaacgttag ccgaaagcgt aagccatctt tttaatgtta ataactagtt aattaaagtg
    41281 gcatcctccc gcatcctctc tgataatgac gggatgccgg gagcaatcat gtctgcttcc
    41341 tgaactttct tctgacagac caatggatgc cagtaatgat tagcggcgtg ctgtacgccc
    41401 tgttagcagg gttgatgtgg gggcttattt ttgtcgggcc gttgatcgta ccggaatacc
    41461 cggcgatgtt gcagtcgatg gggcgttatc tggcgttagg gttaattgcg ctgcccattg
    41521 cctggctggg acgcgtgcgt ctgcgtcagt tggcgcgtcg cgactggctt accgccttga
    41581 tgctcaccat gatgggcaac cttatctatt acttctgcct tgccagtgcc attcaacgta
    41641 ctggcgcgcc tgtttccacg atgattatcg gcaccctgcc ggtggtgatt cccgtttttg
    41701 ccaatctgct ttatagccag cgcgacggca aactcgcatg gggaaaactc gccccggcac
    41761 tggtttgtat tggcatcggc ctggcgtgtg tgaatattgc tgagttaaac cacggactcc
    41821 ccgattttga ctgggcacgt tatacctctg gcatcgtgct ggcgttagtt tccgtggtct
    41881 gctgggcgtg gtatgccctg cgcaacgccc gctggctgcg ggaaaatccc gacaaacatc
    41941 cgatgatgtg ggcaacggcg caggcgctgg tcacactgcc ggtttctctc atcggctatc
    42001 tcgtcgcctg ttactggctg aatatacaaa cgccggactt ctccttacct tttggccccc
    42061 gtccgctggt gtttattagt ctgatggttg cgatagccgt gctttgctca tgggttggcg
    42121 cactctgctg gaacgtcgcc agccagcgat taccgacagt gattctcggg ccgctgattg
    42181 ttttcgaaac gctggcaggt ttgctgtaca cctttttact ccgccagcaa atgccgccgc
    42241 taatgacgct gagcggtatc gcgctgttag tgattggcgt ggtcattgcg gtcagagcaa
    42301 aaccagaaaa acctttaact gaatctgtct cagaaagttg acacgctggc agtgagttaa
    42361 ataagcctct gctacgtaag ggttatagct tttgccttaa agatgcattt aaaatacatc
    42421 ttatattatt aagaatgagg tatcagctat ggcttatcgc gaccaacctt taggtgaact
    42481 ggcgctctct attcctcgcg cttcagctct gtttcgtaaa tatgatatgg attactgctg
    42541 tggcggtaag caaacgctgg cgcgcgcggc ggcacgtaaa gaactggatg ttgaggtcat
    42601 tgaagctgaa ctggcaaagc ttgctgaaca accgattgag aaagactggc gtagcgcccc
    42661 gctggcagaa atcattgacc atatcatcgt gcgctaccac gatcgtcacc gcgagcaact
    42721 gccggagctg atcctgcaag cgactaaagt cgagcgcgtt cacgccgaca aaccgagcgt
    42781 gccaaaaggg ctgacaaaat acctgactat gctgcatgaa gagctatcca gccacatgat
    42841 gaaagaagag cagatcctct tcccgatgat caaacaaggc atgggcagcc aggcaatggg
    42901 accaatcagc gtgatggaaa gcgagcacga tgaagcgggc gaactgctgg aagtgattaa
    42961 acacaccacc aataacgtca caccgccgcc ggaagcctgc accacctgga aagcgatgta
    43021 taacggtatt aatgaactga ttgatgacct gatggatcac atcagcctgg aaaacaatgt
    43081 actgttccca cgcgcgctgg cgggtgagta atctaaaagc tggatggcat tgcgccatcc
    43141 agcatgataa tgcggttatt tccgcagttc agcagcccgc ttcttaccaa taaacaacca
    43201 gcccagcccc agtgcgataa accacagcgg ggtgaccaac agcgcctggc gagtgtcatc
    43261 ttccagtgtc agcaacacga ccacgaacac aaagaacgcc atacataccc agcacatcag
    43321 cttgccgagc ggcatcttgt agatcgactt ctcatgcaga tgaggacgct gtttgcggta
    43381 aaccaggtac gagcaaagga taattgtcca gacgaacata aacagaatcg cggaaacggt
    43441 tgtaatcatc gtgaacgcgc caatcacgct aggattcaca tacaacatca ccacgccgcc
    43501 gagcagacag atacacgaga aggtcaaccc tttcgcgggt actgcgcgct tagaaagttt
    43561 agcgaacgct ttcggtgcca caccttcctg cgccagacca aacagcatac ggctggtaga
    43621 gaagacgccg ctgttagcgg aagacgccgc agaggtcagc accacaaagt tgatcacgct
    43681 ggcagccgca ggcaaaccta ccaacacaaa cagttcaaca aacgggctct tctccgggac
    43741 taccgaactc cacggcgtca cggacataat cacaatcagc gcgaagacgt agaacataat
    43801 gatacggatc ggaatggagt taatcgcgcg tggcagtgat ttctccggat ctttggtttc
    43861 cgcagcggtt gtacctacca gctcaatccc tacgaaagcg aaaaccgcta tctggaatcc
    43921 ggcaaagaag ccacttaaac ctttcgggaa ccagccgcca tcattccaca aatgcgcgaa
    43981 tgacgcttcc acaccagtcg gtgactgaaa gtgcatcgcc accataacca ggccgacgac
    44041 aatcagcgac acaatggcga cgattttgat catcgcaaac cagaactcca tctcaccgaa
    44101 cattttcacg gtggcgagat tgagcgtcag cagcagcact atcaccgcca gcgaggcgac
    44161 ccagtcggag agatcgggga accagaactg agcataagca gtgatcgcca ccacgtctgc
    44221 cataccggtt acaacccagc agaaccagta agtccagccg gtgaaatatc ctgcccacgg
    44281 cccgagtaaa tcggaagcga agtcactaaa agatttgtat tccagattcg aaagcagcaa
    44341 ttcccccatt gcccgcatca cgaaaaagag cataaaaccg atgatcatat aaacgaaaat
    44401 gatcgacggc ccggcaaggc taatcgtttt gccggacccc ataaacaacc ccgtaccaat
    44461 ggcaccgcca atggcaataa gctgaatatg tcggtttgtg agattgcgcc gtagcgactg
    44521 ttcagccgga gcctgatcat cggcaacgac ttttacctga tctaccatgt ttttttcttc
    44581 ctgtacctgt ctgtgttgtt caggctctac ggcctttagt cagtctatga tacgacgaat
    44641 ctgtatcggg actcatcgat attaggtaac aatacgcggg atgaatacta agatttagat
    44701 ctaatgttaa ttttatgttt aaagtgagtg tcatatcacg gtgataacgc gaaacagctc
    44761 acaaaaatac acgcttacgg ggtctttgca acacaaaata gcgccgtttg gcaaactgaa
    44821 gggtttattg ctgaatgcct gctcccctct ggttcgaggg gaacagaatg cgctgcttag
    44881 aggatttcca gcagttcgac ttcaaacacc agggtgctga acggagggat ggatgcgcct
    44941 gcgccgcgct cgccatatgc cagttcctgc gggatagtca gttcccattt agaacctacc
    45001 ggcatcagag tcagtgcttc aatccagcca gggatcacgc cattaaccgg gaattcagcg
    45061 ggttcaccac gagcaacgga gctgtcaaac acggtgccgt cgatcagttt accggtgtaa
    45121 tgaacacgaa cgcggtcggt acgtgccgga attgcgcctt caccctggtt gatcacgcgg
    45181 aattgcaggc cagattcggt gctattcaca ccttcttttt tggcgttttc ttccaggtat
    45241 ttcacacctt cagcagccat cgcctggaaa cgctgacgac gaacggcatc ggcgcgttcg
    45301 tggatttcac gcagcgcgcg atgcaccaca tcaaccggca cagccggatg tttgccttcc
    45361 agcgcatcgg caatacctgc aaccagtgct tctggcagca gcccttccag gccagattca
    45421 ctcagttgtt gccctacctg caaaccaatg ccgtagcttg cttgcgcttc gatggtgtca
    45481 aaagttgggg tggtcatcgt atttcctttc ttagggtata aaagtagcgg gcagcatatc
    45541 agccacactt ctttgggtaa aactttgtcg caggaatgat gacaaatccc ggggaaaaag
    45601 aaacaatagc aatatcccgt atgaatataa taaaggcggg tttaccacgg acatcatagc
    45661 gttagcttat tcataggcta tgattgagga acaagacgcg gagcaggagg aaaccatgcc
    45721 cgggcgcttt gaactaaaac caaccctgga gaaagtctgg cacgcgccgg acaattttcg
    45781 cttcatggac ccgctgccgc cgatgcatcg tcgggggatc attattgccg ccattgtatt
    45841 ggtggtcgga tttctgcttc catctgatga tacgcccaac gcgccggtgg tgacgcgtga
    45901 agcgcagtta gatattcagt cgcaatcaca accgccgaca gaagaacagc tgcgggcgca
    45961 attagtcaca ccacaaaatg atcctgatca ggtggctccg gtcgctccag aacctataca
    46021 ggaaggccag ccagaagaac agccgcaaac tacccaaacg caaccattcc agccagatag
    46081 cggaattgat aatcaatggc gctcttaccg tgtcgaaccc ggtaaaacga tggcgcagct
    46141 gttccgcgac cacggactgc ccgctaccga tgtctatgca atggcgcagg tagaaggcgc
    46201 gggtaagccg ctgagtaatt tgcaaaatgg gcagatggtg aaaatccgcc agaacgccag
    46261 cggcgtggtg acgggcttaa ccattgatac ggggaataat caacaggtcc tgtttacccg
    46321 ccagccggat ggcagtttta ttcgtgcgcg gtagatgaag ggtcaaccaa aagccagcta
    46381 aggattgtct ctttgatgac cttacgtctg gcttccagtg cctggggtga aaccatactg
    46441 atgtgataaa ccaggccaag cgtatgctgg ttgatcaaat aatatccccc aagcgcagca
    46501 atattaatat tcacctgcaa tgggtcgata tctggcttaa aaatattgtg tttcttccct
    46561 tcatccaata aggattccat taactgcaag tgcgcatggt taatttctaa gagacgcagt
    46621 gattttgcat aatgcacacc tttactttga ttttcactat gcacaatttt taaaaaccaa
    46681 gggt
//
LOCUS       DAJTZL010000008        43713 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.008, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000008 DAJTZL010000000
VERSION     DAJTZL010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 43713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43713
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.008"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..559)
                     /locus_tag="OO544_000427"
     CDS             complement(<1..559)
                     /locus_tag="OO544_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HCR6766125.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPDTARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVLLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQR"
     gene            complement(762..998)
                     /gene="feoC"
                     /locus_tag="OO544_000428"
     CDS             complement(762..998)
                     /gene="feoC"
                     /locus_tag="OO544_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HCR6766126.1"
                     /translation="MASLIQVRDLLALRGRMEATQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            complement(998..3319)
                     /gene="feoB"
                     /locus_tag="OO544_000429"
     CDS             complement(998..3319)
                     /gene="feoB"
                     /locus_tag="OO544_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HCR6766127.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTSARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            complement(3336..3563)
                     /gene="feoA"
                     /locus_tag="OO544_000430"
     CDS             complement(3336..3563)
                     /gene="feoA"
                     /locus_tag="OO544_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HCR6766128.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            complement(4001..6322)
                     /locus_tag="OO544_000431"
     CDS             complement(4001..6322)
                     /locus_tag="OO544_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HCR6766129.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYIDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKVAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFKNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNARRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            complement(6419..6895)
                     /gene="greB"
                     /locus_tag="OO544_000432"
     CDS             complement(6419..6895)
                     /gene="greB"
                     /locus_tag="OO544_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HCR6766130.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCMENLKIVNYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            7123..7842
                     /gene="ompR"
                     /locus_tag="OO544_000433"
     CDS             7123..7842
                     /gene="ompR"
                     /locus_tag="OO544_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HCR6766131.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            7839..9191
                     /gene="envZ"
                     /locus_tag="OO544_000434"
     CDS             7839..9191
                     /gene="envZ"
                     /locus_tag="OO544_000434"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HCR6766132.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(9267..10889)
                     /gene="pckA"
                     /locus_tag="OO544_000435"
     CDS             complement(9267..10889)
                     /gene="pckA"
                     /locus_tag="OO544_000435"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HCR6766133.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            11268..11582
                     /locus_tag="OO544_000436"
     CDS             11268..11582
                     /locus_tag="OO544_000436"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCR6766134.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATTGMSGWLKWQTDGMNPWRAEKAIWD
                     FG"
     gene            11595..12968
                     /locus_tag="OO544_000437"
     CDS             11595..12968
                     /locus_tag="OO544_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001241106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HCR6766135.1"
                     /translation="MAMLLLPWIQQSLRIRNGSSRYSYFYQSVWHNVLILLVIFLSNG
                     LTWLVLLLWGELFKLVGITFFNTLFFATDWFMYLTFGLVTALVVILARTQSRLIDSIQ
                     KLFTLIATGLLPLVSLLTLMFIITLPFTDLSAISRHISAAGLLLTLAFLQLILMAIVR
                     DPQKASLPWTGPLRCLIKTALLVAPLYVFVAAWALWLRVAQYGWTVDRLQGALAVLVL
                     LVWSLGYFVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMA
                     RYQSGKNTPDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQHLQ
                     EQVSEKVLAENVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGQA
                     ERILFAFNDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLDLTVDGET
                     LEINLSK"
     gene            complement(13103..13981)
                     /gene="hslO"
                     /locus_tag="OO544_000438"
     CDS             complement(13103..13981)
                     /gene="hslO"
                     /locus_tag="OO544_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HCR6766136.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(14006..14407)
                     /gene="hslR"
                     /locus_tag="OO544_000439"
     CDS             complement(14006..14407)
                     /gene="hslR"
                     /locus_tag="OO544_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HCR6766137.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMALAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            complement(14418..15086)
                     /gene="yrfG"
                     /locus_tag="OO544_000440"
     CDS             complement(14418..15086)
                     /gene="yrfG"
                     /locus_tag="OO544_000440"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HCR6766138.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            complement(15151..17286)
                     /gene="igaA"
                     /locus_tag="OO544_000441"
     CDS             complement(15151..17286)
                     /gene="igaA"
                     /locus_tag="OO544_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HCR6766139.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIYTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDGSRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMKIQAYYESCLNPQLITPSESLIE"
     gene            17606..18166
                     /gene="nudE"
                     /locus_tag="OO544_000442"
     CDS             17606..18166
                     /gene="nudE"
                     /locus_tag="OO544_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HCR6766140.1"
                     /translation="MSKSLQKPTILNVETVAHSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            complement(18331..20883)
                     /gene="mrcA"
                     /locus_tag="OO544_000443"
     CDS             complement(18331..20883)
                     /gene="mrcA"
                     /locus_tag="OO544_000443"
                     /EC_number="2.4.1.129"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HCR6766141.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQEATAMLADGSTVALSMDGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGVGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            21003..21782
                     /gene="hofM"
                     /locus_tag="OO544_000444"
     CDS             21003..21782
                     /gene="hofM"
                     /locus_tag="OO544_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HCR6766142.1"
                     /translation="MAFKIWQIGLHLQQQEAVAVAIVRGAKECFLQRWWRLPLENDII
                     KDGRIVDAQRLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRCRWGRKLAVGMTSAKELAAALSVDPES
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            21782..22321
                     /gene="hofN"
                     /locus_tag="OO544_000445"
                     /pseudo
     CDS             21782..22321
                     /gene="hofN"
                     /locus_tag="OO544_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312263.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
     gene            22305..22745
                     /gene="hofO"
                     /locus_tag="OO544_000446"
     CDS             22305..22745
                     /gene="hofO"
                     /locus_tag="OO544_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HCR6766143.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWATLYRLVDTTPFSEEKTLPFSPLDFQLPGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            22735..23139
                     /gene="hofP"
                     /locus_tag="OO544_000447"
     CDS             22735..23139
                     /gene="hofP"
                     /locus_tag="OO544_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HCR6766144.1"
                     /translation="MRVKRWLLAGIVLCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDVLTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            23051..24289
                     /gene="hofQ"
                     /locus_tag="OO544_000448"
     CDS             23051..24289
                     /gene="hofQ"
                     /locus_tag="OO544_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HCR6766145.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNNNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            24690..25211
                     /gene="aroK"
                     /locus_tag="OO544_000449"
     CDS             24690..25211
                     /gene="aroK"
                     /locus_tag="OO544_000449"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HCR6766146.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            25268..26356
                     /gene="aroB"
                     /locus_tag="OO544_000450"
     CDS             25268..26356
                     /gene="aroB"
                     /locus_tag="OO544_000450"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HCR6766147.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLKRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEIRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            26448..27734
                     /gene="damX"
                     /locus_tag="OO544_000451"
     CDS             26448..27734
                     /gene="damX"
                     /locus_tag="OO544_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HCR6766148.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTSSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPSTTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPTATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETARNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            27841..28677
                     /gene="dam"
                     /locus_tag="OO544_000452"
     CDS             27841..28677
                     /gene="dam"
                     /locus_tag="OO544_000452"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HCR6766149.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKTYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            28695..29372
                     /gene="rpe"
                     /locus_tag="OO544_000453"
     CDS             28695..29372
                     /gene="rpe"
                     /locus_tag="OO544_000453"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HCR6766150.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            29365..30123
                     /gene="gph"
                     /locus_tag="OO544_000454"
     CDS             29365..30123
                     /gene="gph"
                     /locus_tag="OO544_000454"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HCR6766151.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPIAGEE
                     RVITWIGNGADVLMERALTWARQERATQRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            30116..31120
                     /gene="trpS"
                     /locus_tag="OO544_000455"
     CDS             30116..31120
                     /gene="trpS"
                     /locus_tag="OO544_000455"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HCR6766152.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            31280..32185
                     /locus_tag="OO544_000456"
     CDS             31280..32185
                     /locus_tag="OO544_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6766153.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLTQKGLCLALE
                     LGPHTYVGEHQLICRKGESANVKRVGLDNRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            32202..32564
                     /locus_tag="OO544_000457"
     CDS             32202..32564
                     /locus_tag="OO544_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR6766154.1"
                     /translation="METRLNLLCEAGVIDKDICKGMMQVVNVLETECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYLLANLYGLWMAANEEV"
     gene            32648..33811
                     /locus_tag="OO544_000458"
                     /pseudo
     CDS             32648..33811
                     /locus_tag="OO544_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417840.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
     gene            33811..35037
                     /locus_tag="OO544_000459"
                     /pseudo
     CDS             33811..35037
                     /locus_tag="OO544_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417839.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
     gene            35034..35912
                     /locus_tag="OO544_000460"
     CDS             35034..35912
                     /locus_tag="OO544_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HCR6766155.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLGVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRETPSQFFQ"
     gene            35923..36276
                     /locus_tag="OO544_000461"
     CDS             35923..36276
                     /locus_tag="OO544_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HCR6766156.1"
                     /translation="MKKIGVAGLQREQIKKTIEATATGSFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            36288..37592
                     /locus_tag="OO544_000462"
     CDS             36288..37592
                     /locus_tag="OO544_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HCR6766157.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIRPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVMRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPIVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            37604..38689
                     /locus_tag="OO544_000463"
     CDS             37604..38689
                     /locus_tag="OO544_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCR6766158.1"
                     /translation="MKTFPLQSLTIIEAQQKQFALVDSICRHFPGSEFLTGGDLGLTP
                     GLNQPRVTQRVEQVLADAFHAQAAALVQGAGTGAIRAALAALLKPGQRLLVHDAPVYP
                     TTQVIIEQMGLTLITADFNDLSALKQVVDEQLPDAALVQHTRQQPQDGYILADVLATL
                     RSAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVISRIRS
                     TLYSGGSQIQGAQALEVLCGLVFAPVMHAVQAGVSERLLALLNGGAVPEVKSAVIANA
                     QSKVLIVEFHQPIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQSE
                     HCAIRINPNRNGEETVLRILRESIASI"
     gene            complement(38841..39572)
                     /gene="phoB"
                     /locus_tag="OO544_000464"
     CDS             complement(38841..39572)
                     /gene="phoB"
                     /locus_tag="OO544_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator PhoB"
                     /protein_id="HCR6766159.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(39672..40457)
                     /gene="frlD"
                     /locus_tag="OO544_000465"
     CDS             complement(39672..40457)
                     /gene="frlD"
                     /locus_tag="OO544_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HCR6766160.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TLVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            complement(40454..41285)
                     /gene="frlC"
                     /locus_tag="OO544_000466"
                     /pseudo
     CDS             complement(40454..41285)
                     /gene="frlC"
                     /locus_tag="OO544_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026213.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
     gene            complement(41335..42357)
                     /gene="frlB"
                     /locus_tag="OO544_000467"
     CDS             complement(41335..42357)
                     /gene="frlB"
                     /locus_tag="OO544_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HCR6766161.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKPLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            complement(42378..>43713)
                     /gene="frlA"
                     /locus_tag="OO544_000468"
     CDS             complement(42378..>43713)
                     /gene="frlA"
                     /locus_tag="OO544_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
                     /protein_id="HCR6766162.1"
                     /translation="GSQELQRKLGFWAVLAIAVGTTVGSGIFVSVGEVAKAAGTPWLT
                     VLAFVIGGLIVIPQMCVYAELSTAYPENGADYVYLKNAGSRPLAFLSGWASFWANDAP
                     SLSIMALAIVSNLGFLTPIDPLLGKFIAAGLIIAFMLLHLRSVEGGAAFQTLITIAKI
                     IPFTIVIGLGIFWFKAENFAAPTTTAIGATGSFMALLAGISATSWSYTGMASICYMTG
                     EIKNPGKTMPRALIGSCLLVLVLYTLLALVISGLMPFDKLANSETPISDALTWIPALG
                     STAGIFVAITAMIVILGSLSSCVMYQPRLEYAMAKDNLFFKCFGHVHPKYNTPDVSII
                     LQGALGIFFIFVSDLTSLLGYFTLVMCFKNTLTFGSIIWCRKRDDYKPLWRTPAFGLM
                     TTLAIASSLILVASTFVWAPIPGLICAVIVIATGLPAYAFWAKRSRQLNALS"
BASE COUNT        10157 a        11128 c        11988 g        10440 t
ORIGIN      
        1 cgcgttgacg aatatgtttt tgaattaact ccagcagcgc catacgtcgg tgctgcatga
       61 tttcatcatc aggcatgacg gtgacatcaa tcagcggaaa tgcggaggca taaagctgcc
      121 ttgccagttt gggatcggcg aaacaatcca gccaacacag cgaataggga taggggcttt
      181 caataccgtg gtaaaacaac aatagcacca ccatcggcaa cgttttgtat ccagcatcaa
      241 ggtgattttg cattgcggca atagcgtaac gcatcatgcg aaatgcgatc agtttgtttg
      301 aggtgctttg atgttcaatc agacaataga tgtatcctgg tccttgttcc gttttcaccg
      361 accacagcac atcggaatag ctttcacgca gatcgtcatc aataaagctg ctcgactcca
      421 gcttgagggt tttcatatca caaagcgcgt gaatgggtgc cggtaaatga aacgcaagaa
      481 aatcacgagc cgtgtccggt tggcgtaaaa agagtttgaa cagcgcatcg tgtggggtgg
      541 aactctgctt tttgctcatg gcgttccttc acctgttgtt tgaatgccgc gactctacgc
      601 gccactccac acctgtgcat tagcgagttt tcatccctgc gagatgcttt caaaggaact
      661 tacgccgtgc tgcaagagtt gcatttttat ggaaagccgc acgcaaaaaa gcccggatgc
      721 agacgcatac cgggctttcc aaaacaggcg atggagtaag gttaacgcag cgcccaccac
      781 tcgcgcagac aggcttttcc ttccgggcag cttttacaac tgccagagag gcagccgtca
      841 ggttcttcct gaatccgcac ggctttgccc atactttcca gttgttgcag catggcgtta
      901 atcattggct gcggagtgtt caatgtctgg cttatctggg tcgcttccat acggccccgt
      961 aacgccagca aatcacgtac ctgaataagt gaagccatta atggcaatca ccggtggtgc
     1021 tggctgcgca gcaactgctt accgacttgc gggttgccag cagttcgata tccacccggc
     1081 tacgcgcgcg gcgcagcaga ccgataacta cgatgttaaa caggattacc gccagaatgc
     1141 acaccaggct gtaagttggg tgctgactgt agctggcgac ctgatagaac aatgttgcca
     1201 gtgagtaagc gatattcagc ccccacagga tggagaagcc catccagcca cggcttgatt
     1261 cacgggcgat ggctcccatc accgagatac atggtacata cagcaggacg aaaatcaggt
     1321 agctgtatgc ggcagctgcg ctaccgaatt tctgatccat cacgcccatc gccccggtac
     1381 ccatttcacc gtcgcctttg ctagcttcaa tggggttcat cagtacgcta aggctgaagg
     1441 tgtctttcag gctctgccag gtttcatcta ccgcactgaa cagctcttca ccgaggttga
     1501 attccgccgg attgaactct tcgtcctgaa tattttctgc ggtgtagagg gtgttgagcg
     1561 tacccaccac cacttctttt gccattgcgc ccgtaaacag gccaaccgtt gcctgccagt
     1621 tatcttcatg tacgccaatc ggcttgaaga caggggttat cacccggctg acggacgcca
     1681 gcgccgagtc gttgatgtta tcgacgattt tcccgctcaa cgagaagctg ttgaaagcgc
     1741 tcaggaaaat gctgacgatg ataatcactt taccagcacg cagaacgaag cccttcagac
     1801 gctgccaggt ctggataatc aggcttttaa cgtgtggtac atggtagacc ggcagctcca
     1861 tgacaaacgg cgtcgcttca ccgcgcatga tggtgtactt gagcatcagg ccagtcagca
     1921 ccgccatcac gataccgagc atatacagcg agaaaaccgc cagggcaccg ttctgcccga
     1981 agaaggcagc cgcgaatact gcgaagatag ccagacgcgc gccgcaggac ataaacggtg
     2041 ccatcatgat ggtcatcaga cgttcacgcg gtgcatcaag cgtacgtgcg cccattaccg
     2101 acggtacgtt acaaccgaaa ccgacgatca gcggcacaaa ggatttcccc ggcaagccca
     2161 gcgcctgcat caggcggtcc atcacaaacg ctgcacgcgc catatagccg gagtcctcaa
     2221 ggaaggagag gaacaggtac atcatgccga tctgcggcac cagtggcagc acggtgttaa
     2281 taccgccacc caggccctgg gcgaggaaga tagtcagcca gtccgggaag tggagcgtgt
     2341 agccaatcca ttgaatacca tgcacaaata gcgccacgga gccgacgtca aacagcggct
     2401 gtaacgcccc gccgatgttg atagccagca ggaacatcag gtacatcaca aagaggaaaa
     2461 tcggcagacc gaggaaacgg ttgagcacga ttttatctac cgcagtggtg aaacggctgg
     2521 gttctgccgt cagggtgttg cttaccacat cacagatggc agctatgcac tggtaacgcg
     2581 catcggcaat gtgcagcgcc ggatcatcca tttcattacg cagacgggca agggcggcat
     2641 ccagatgctg cgacgcttca ccggcgtagg cgcggctgta gatatcgcct tccagcattt
     2701 gcaggcccag ccagcgacgt tgtttcagcg ggatgtcgga aggcatcact tttgccagtg
     2761 aatctgcttc gttgagtagc ggctgtgcgt aatgcaccag ttccacattc tcgttagctt
     2821 tatagcgatc aatcgccagc ttgagcgctt caataccgcg accacgggtt gaaaccagcg
     2881 ggatcactgg gcagccaaga cgcgccgaca gagcatcaat ttcaatacga atattttgct
     2941 tctcggcaat gtcgagcatg ttcagtgcca cgatgcaggg aatgccgagt tccagcagtt
     3001 gtagcgtcag gtacaggtta cgctcaaggt tagacgcatc caccacgtta atcagcaggt
     3061 cggcgtcgcc actcaaaatg tagtgacagg cgatttgctc atcgagcgag gtctgcgatg
     3121 agatggtggt cagagaatag gtgccgggca ggtccaccag cgtgacctga tgatcggtgg
     3181 tggagaattg cccttcttta cgttcgacgg taacgccagc ccagttacct acacgctgac
     3241 gtgcgctagt gagctggtta aataaggttg tcttgccaga atttggatta ccaattaagc
     3301 caatggttaa ttttttcatt gttgttatca ccgtattaac aggaaaccgc ttccacttct
     3361 aataaggcca gatctttttt gcgtaatacc aggctcacac gacgggtttc gatatgaatg
     3421 gggtcgccga gtggagcgac gcgcaccaca ttaaaagagg agccaggtaa catgccaaga
     3481 gaaagcagtt tttggcgata tgccgggctg atttcacggg aaaagccagt gattttccac
     3541 gcagtatctg gagtgtattg cataggtgcc tacttgtttc tcattaactg gataactacc
     3601 tcatggggcg tttgacagcg acatgagacc agatgatttg ccaacaaagg agagattaaa
     3661 taatcaacaa cacaatgata atgagaatgg tttctatcag caatacttaa aatgtgtgcg
     3721 aatgttggtt tgaataataa tctgactcaa tgttgatgtg gctcaaggtt atatcgcagc
     3781 gaaaatataa ttcgaatagt gaattacccg gactggctaa tgtttaatta aggtttcagt
     3841 tgttaatgaa gtgataaata ttgccggaat atattatgag tttggttgtg gaataaaaag
     3901 gccggagata ttctccggca cttttatgtc aggcaaggcg ttcgttgcca gatgcggcgt
     3961 gaacgcctta tcgcgcaggt ttaggggcca gtccgggcgt ttaacgtttt ttgcccattg
     4021 ccgccgccag cgcatccatc atcgcgctat taccggcagg ctgcgcttca cgaccgcgtg
     4081 gtttggctgc cgggcggttg ttttgcgggc gttcattacc gccgccgcga cgggcgttgg
     4141 tttcgccagg ctgctcgtcc agacgcatgg tcagggcgat acgtttacgc tgaagatcca
     4201 cttccagcac tttcaccttc acaatgtcgc ccgctttcac cacggtatgc ggatcttcca
     4261 caaacttgtt cgacaatgaa gagatgtgaa ccaggccgtc ctgatgcacg ccaatatcga
     4321 caaacgcgcc aaagttggtg acgttggtga cagcgccttc gaggatcata cccggttgca
     4381 ggtcgttcat tgtctcgacg ccatcggcaa actgagcggt tttaaattcc ggacgcggat
     4441 cgcgacccgg tttttccagc tctttgatga tgtcagttac tgtcggcaca ccgaattttt
     4501 catcagtaaa gtcagacgct ttcaggttac gcagttcgct gctgttaccc atcagatctt
     4561 tcagtgcctg ctgtgttgct gccagaatgc gttccaccac cggataggct tccgggtgaa
     4621 cggtagaggc gtccagcggg ttatcaccgt ggttaatgcg caggaagccc gcgcactgct
     4681 cgaaggcttt cggccccaga cggctgactt tcagcagttg ctgacggttc ttgaactggc
     4741 cgttctcatc gcgccaggcc acgatgtttt gcgccatcat gcgcgtcagg cccgccacgc
     4801 gagttaatag cggaacagaa gcggtgttga gatcgacgcc aacggcgttt acgcagtctt
     4861 ctactactgc atccagtttg cgggccagtt gcgtctggct gacgtcatgc tgatactgac
     4921 ctacgccgat agatttcgga tcgattttca ccagctccgc cagcggatcc tgcaaacggc
     4981 gggcgataga taccgcacca cgcagcgaaa cgtcgagatc cgggaactcc tgcgctgcca
     5041 gctcggaagc tgagtaaacc gacgcgccag cttcgctgac gatcactttc tgtgcggtca
     5101 ctttcgggaa ctgcttctgc acgtcgagat agaaacgttc agtttcgcgg gaagctgtac
     5161 cgttgccgat cgccaccagt tcaacgttat gtttttcaca caaggcagca acggtcatcg
     5221 ctacttttgc ggcttgtccg gtgtgcgggt aaatggtatc ggtcgccacc agtttgccag
     5281 tggcatcgac cactgccact tttaccccgg tacgcagacc cggatcgagg cccatcgttg
     5341 cacgcagtcc ggcaggggcc gccatcagca gatcgtgcag gttacgggca aagacgttga
     5401 ttgcttcatc ttccgcgcgt tcgcgcacgg tacccatcag ttcggtttcc agatgcatca
     5461 gcaccttgat gcgccaggtc cagctcacta cgcctttgcg ccagctatcc gccggggcat
     5521 tgttcaggcg caggccaagg tgatccatga tgatttgctc gcaatagctc tctttgggcg
     5581 gctcatcgaa ctgcggatcg gcattcagcg aaagctggag tacgccttcg ttacgcccac
     5641 ggaacatcgc cagcgcgcgg tgagaaggca ccgtggacaa cggttcgtga tgatcgaaat
     5701 agtcgcggaa tttcgcccct tcctcttctt taccgctcac caccgtagaa accaaatgcg
     5761 cgttcttcca cagataatca cgcactttcg ccagcagcgc ggcatcttcg gcaaaccgtt
     5821 ccatcaggat atagcgcgcg ccgtccagcg cggctttggt atctgccacg cctttatcgg
     5881 catcaatata ttgcgcagcg gcgacttctg gtgtgtgtga cggatcgctc cacagcaggt
     5941 cagccaacgg ctcaagccct gcttcaatgg cgatttgccc gcgggtgcgg cgtttaggtt
     6001 tgtagggcag gtagaggtct tcgagttcgg ttttgcttag ggtggcgttg atggccttcg
     6061 ccagatcatc ggtgagtttg ccttgctcgg aaatggactt gaggatcgcc tgacgtctct
     6121 cttccagctc gcgcagatag ctcagacgcg tttccagatt acgcagctgc gtgtcatcca
     6181 gaccgccggt gatttcctta cgataacgtg cgataaacgg cacggtattc ccttcgtcaa
     6241 gcaggcgaac ggcagcgtca acctgttccg ggcgcgcctg aatttcaccc gcaataatgc
     6301 ggcagaacga atcattcatc atggtttgag ttcatctttt cggatcaaaa atcaggggat
     6361 agttatacgg actggctggc aaaaatgcca gccatcggca ggaggttaag actcttcatt
     6421 acggtttcac gtattcgata gcattaacat accagctcgc ttccccggca ggggtattca
     6481 ccaccgccag atcgccgact tcttttttca gcaatgcgcg ggccatcggg gaatcgatag
     6541 agatgtaatc tttacggcca aaaatttcat cgtagccgac aatacggaaa cggtgagtca
     6601 cgccatcgtc gttttcaatc tccacccacg cgccaaaaaa gactttgcct tcctgctgag
     6661 gggagtaatt gacgattttg agattttcca tgcatttagt gagatagcgc acgcgacggt
     6721 cgatttcacg cagacgcttt ttattatact gatagtcagc attttcgctg cggtcgccca
     6781 gacttgcggc ccaggtcacc ttttttgtga cctccgggcg ttcttcacgc cagagataat
     6841 taagctcttg tttgagtttt tcataccctt cccgggtaac caggggcgtt ttcatctcgt
     6901 tgattccctt tgtctgtttg ataatgcgca cattgggtat aacgtgatca tatcaacaga
     6961 atcaataatg tttcgccgaa taaattgtat acttaagctg ctgtttaata tgctttgtaa
     7021 caatttaggc tgaaattcat accagattta gctggtgacg aacgtgagct tttttaagaa
     7081 tacacgctta caaattgttg cgaacctttg ggagtacaaa caatgcaaga gaactacaag
     7141 attctggtgg tcgatgacga catgcgcctg cgtgcgctgc tggaacgtta tctcaccgaa
     7201 caaggcttcc aggttcgaag cgtcgctaat gcagaacaga tggatcgcct gctgactcgt
     7261 gaatctttcc atcttatggt actggattta atgttacctg gtgaagatgg cttgtcgatt
     7321 tgccgacgtc ttcgtagtca gagcaacccg atgccgatca ttatggtgac ggcgaaaggg
     7381 gaagaagtgg accgtatcgt aggcctggag attggcgctg acgactacat tccaaaaccg
     7441 tttaacccgc gtgaactgct ggcccgtatt cgtgcggtgc tgcgtcgtca ggcgaacgaa
     7501 ctgccaggcg caccgtcaca ggaagaggcg gtaattgctt tcggtaagtt caaacttaac
     7561 ctcggtacgc gcgaaatgtt ccgcgaagat gagccgatgc cgctcaccag cggtgagttt
     7621 gcggtactga aggcactggt cagccatccg cgtgagccgc tctcccgcga taagctgatg
     7681 aaccttgccc gtggtcgtga atattccgca atggaacgct ccatcgacgt gcagatttcg
     7741 cgtctgcgcc gcatggtaga agaagatcca gcgcatccgc gttacattca gaccgtttgg
     7801 ggcctgggct acgtctttgt accggacggc tctaaagcat gaggcgattg cgcttctcgc
     7861 cacgaagttc atttgcccgt acgttattgc tcatcgtcac cttgctgttc gccagcctgg
     7921 tgacgactta tctggtggtg ctgaacttcg cgattttgcc gagcctccag cagtttaata
     7981 aagtcctcgc gtacgaagtg cgtatgttga tgaccgacaa actgcaactg gaggacggca
     8041 cgcagttggt tgtgcctccc gctttccgtc gggagatcta ccgtgagctg gggatctctc
     8101 tctactccaa cgaggctgcc gaagaggcag gtctgcgttg ggcgcaacac tatgaattct
     8161 taagccatca gatggcacag caactgggcg gcccgacgga agtgcgcgtt gaggtcaaca
     8221 aaagttcgcc tgtcgtctgg ctgaaaacct ggctgtcgcc caatatctgg gtacgcgtgc
     8281 cgctgaccga aattcatcag ggcgatttct ctccgctgtt ccgctatacg ctggcgatta
     8341 tgctattggc gataggcggg gcgtggctgt ttattcgtat ccagaaccga ccgttggtcg
     8401 atctcgaaca tgctgctttg caggtcggta aagggattat tccgccgccg ctgcgtgagt
     8461 atggcgcgtc cgaggtgcgt tccgttaccc gcgcctttaa ccatatggcg gctggtgtta
     8521 agcaactggc ggatgaccgc acgctgctga tggcgggggt aagtcacgac ttgcgcacgc
     8581 cgctgacgcg tattcgcctg gcgactgaga tgatgagcga gcaggatggc tatctggcag
     8641 aatcgatcaa taaagatatc gaagagtgca acgccatcat tgagcagttt atcgactacc
     8701 tgcgcaccgg gcaggagatg ccgatggaaa tggcggatct taatgcagtg ctcggtgagg
     8761 tgattgctgc cgaaagtggc tatgagcggg aaattgaaac cgcgctttac cccggcagca
     8821 ttgaagtgaa aatgcacccg ctgtcgatca aacgcgcggt ggcgaatatg gtggtcaacg
     8881 ccgctcgtta tggcaatggc tggatcaaag tcagcagcgg aacggagccg aatcgcgcct
     8941 ggttccaggt ggaagatgac ggtccgggaa ttgcgccgga acaacgtaag cacctgttcc
     9001 agccgtttgt ccgcggcgac agtgcgcgca ccattagcgg cacgggatta gggctggcaa
     9061 ttgtgcagcg tatcgtggat aaccataacg ggatgctgga gcttggcacc agcgagcggg
     9121 gcgggctttc cattcgcgcc tggctgccag tgccggtaac gcgggcgcag ggcacgacaa
     9181 aagaagggta aataaacggg aggcgaaggt gcctcccgtt ttgctttcta taagatactg
     9241 gatagatatt ctccagcttc aaatcattac agtttcggac cagccgctac cagcgcggca
     9301 cccgcagggg tgtcggtgta tttatcgaag ttgtcgataa acagtttcgc cagggtttcg
     9361 gctttttcct gccactgttc cggagaagcg taggtgttac gcggatcgag aatcttcgtg
     9421 tctacgcccg gcagttcggt tgggatcgcc aggttaaaca tcggcagagt gaaggtttct
     9481 gcattatcca gcgaaccgtt gaggatggcg tcgataatgg cgcgggtatc tttaatcgag
     9541 atacgtttgc cagtgccgtt ccagccagtg ttaaccagat aagcctgcgc gcccgccgcc
     9601 tgcatacgtt tcaccagcac ttctgcgtac tgagtcgggt gcagcgacag gaacgccgcg
     9661 ccgaagcaag cggagaaggt tggcgtcggt tcggtgatgc cacgctcagt accggccagt
     9721 ttggcggtga agccagagag gaagtgatac tgggtttgat cggcagtcag gcgagaaacc
     9781 ggcggcaaca cgccgaaagc atcagcagtc aggaagataa ccttagtcgc gtggcccgct
     9841 ttggaaaccg gcttaacgat gttatcgatg tgatagattg gataagaaac gcgggtgttc
     9901 tcggtttttg aaccatcatc aaagtcgata gtgccatctt cacgcacggt gacgttttcc
     9961 agcagcgcat cacgacggat agcgttgtag atttcaggtt ccgcttcttt cgacagcttg
    10021 atagtttttg cgtagcagcc gccttcgaaa ttaaacacgc cgtcatcgtc ccagccgtgt
    10081 tcgtcatcgc caatcaggcg gcgtttcggg tcggtggaaa gggtggtttt accggtgccg
    10141 gaaaggccga agaacaccgc aacatcgcct ttctcgccaa cgttggcgga gcagtgcata
    10201 gaagcgatac ctttcagcgg cagcaggtag ttcatcatcg agaacatccc tttcttcatt
    10261 tcgccgccgt accaggtgcc gccaatcagc tgcatgcgct cggtcaggtt aaacgccacg
    10321 aagttttcgg agttcagacc ctgttctttc cactgcgggt tagtgcactt cgcgccgttc
    10381 ataacgataa agtctggttt gaaacctgcc agctcttcat cgctcgggcg aataaacatg
    10441 tttttgacaa aatgcgcctg ccaggccact tcggtgatga aacggacgga aagacgagta
    10501 tccgggttcg caccacagaa agcgtcgaca acgaacagac gtttgccgga aagctgcctg
    10561 gtcaccaggc ctttcagatg ctgccaggtt tccggagaga gaggtttgtt gtcgttctta
    10621 cctttgcctt tgtctgccca ccagaaagta tcgcgagtgg tatcgtcacg gacgatatac
    10681 ttatcttttg gtgaacgacc ggtgaagatc ccggtatcga cggcaacggc acccagatta
    10741 gttaacaccc cgcgctcata acctgtcagg ctcggatcga gctcttcctg atacagcagg
    10801 tcgtagcttg ggttgtaaac gatatcatgt acgtcactga taccataagc ctcgagttct
    10861 tgcggggtca aaccattgtt aacgcgcatt tcactgctcc ttagccaata tgtattgcct
    10921 gaatagtaaa gtctttttgg gggtgttaac cgcgacaagg ctcatagatt tacgtatctg
    10981 gagaaattca tatttgacaa aaaactccgt gattcctgtc acgaaacggt tgctattatc
    11041 gcagaaatag cattttaggt gtggaaaatg ttctcaaaag gttaaatctt ggtttatatg
    11101 gcgggaatat tgtgagtgga atcgcattcc tgaatgaaat tgatgcaaat tcaaccggta
    11161 aagtatcgat tcaccacgct aacttttttt gattattttc tgaaaaaagc aaaatactac
    11221 tgcccggatg cgataattaa cccacctttt gattctggat aaccaacatg gataacgttg
    11281 aactttcacc ggcaacacgc tgggggatga ttgctaccgg attacttcag gggctggttt
    11341 gctacctgct tatcgcctgg cttgccggaa aaaatcacag ttggattgtt tatggcgtgc
    11401 ctgcgaccgt cgcgttttca tccgttttac tcttctccgt gatctctttt aaacagaagc
    11461 gcctttgggg atggctggcg ctggtgttta ttgccacgac agggatgagc ggttggctga
    11521 agtggcaaac tgatggcatg aacccctgga gagctgaaaa ggcgatttgg gattttggct
    11581 gatatctgct gttgatggca atgttgttgc taccgtggat acaacaaagc cttcgtatcc
    11641 gcaatggcag tagccgctat agctactttt accagtcagt atggcataac gtacttatat
    11701 tactggtgat ttttctctcc aatggcctga cgtggctggt gcttttactg tggggtgagt
    11761 tgtttaaact tgttggcatc acgtttttca atacgctctt ttttgcaacc gactggttta
    11821 tgtatcttac atttggtctg gttaccgcac tggtggtgat cctcgcgcgt acacagtcac
    11881 gtttaatcga ctctattcaa aagttgttca cgctaatcgc cacggggttg ctgccgttag
    11941 tatcattgct aaccctgatg tttatcatca ccctgccgtt tacggacctg agcgcgattt
    12001 ctcgccacat ctccgccgcc gggttgctgt taacgctggc ctttttgcaa ttgatcttaa
    12061 tggctattgt ccgcgatccg caaaaagcgt cacttccctg gacggggccg ttgcgttgcc
    12121 tgattaaaac cgctttgctg gttgctccgc tgtatgtgtt cgtcgccgcc tgggcgttat
    12181 ggctgcgggt cgctcagtac ggctggactg tcgaccgctt gcagggcgcg ctggcggtgc
    12241 tggtgttact ggtgtggtcg ctggggtatt ttgtcagtat cgtctggcgt aatgggcaaa
    12301 atccccttgt tcttcagggc aaagtgaacc ttgcggtttc gttattggtg ttggtgatac
    12361 tggtgcttct taattcgccg gtgctggaca gtatgcgcat tagcgtgaac agtcatatgg
    12421 cgcgttatca gagcggcaaa aacacgccag accaggtaac tatctacatg ctcgaacaga
    12481 gcggtcgcta tggacgtgcg gcgcttgagt cgctgaaaag cgatgccggg tttatgaaag
    12541 acccgaaacg cgcgcgggat ctgctgatgg cgttagatgg agagcaacat cttcaggaac
    12601 aggtatcgga aaaagtatta gccgaaaatg tgttaattgc tcctggttct gttaaaccgg
    12661 atgcgacatt ctggtcggcc ttaatccagg atcgctataa cgtgatgacc tgtattgaaa
    12721 aagacgcctg cgtcctggtc gagcaagatc tgaatagtga tggtcaggcg gagcggatcc
    12781 tgtttgcttt taatgatgac agagtcattg tctatggctt tgactcagac agaaaagaat
    12841 gggacgcgct tgatatgagt ttacttccga acgaaataac gaaagaaaaa ttactcacag
    12901 ctgcgaagga tggaaaactg gatctgacgg tagatggtga aacgctggaa ataaacttaa
    12961 gtaagtgaca actcttttaa cggatacgac gctgtatctg accaacaata atttgcgatt
    13021 tatttcacag gcctggtaag cgcagcacat caggccatta cgtcgtaaaa tccggcagag
    13081 ccctctctgc cggacatact cattaatgaa cttgcggatc tgccggagac gcgttgttgc
    13141 ggatttcagc aatatccatc gcattgaaca gatagtggtt accgcagtaa tcacaatgca
    13201 tgtcaatttc gccatcttcc gccaggatgc tatcgacttc ttcatcaggc agcgttttca
    13261 gcgcgtcggc gcaacgttca cgcgagcagg tgcatttgaa ctccacatcc tgcggatcgt
    13321 aaaccgtcac ctcttcttcg tgatacaaac gccacaacac ttcgtttgcc ggtaaggtca
    13381 gcagttcttc ggttttgatg gtttcggtta gcgtcgccag gtggtcaaag tcgtcctgct
    13441 gggcattttg cgcaggcatt acctgcaaca acataccgcc tgcagccggt ttgccgtcta
    13501 cgtcgccggt gcgaataaac aggcgcgtcg gcagctgttc agaacgcata aagtaatctt
    13561 ccaggcaggc cgccagggta tcaccttcca gaccaaccac gccctgatag cgttcgcctt
    13621 cgctcggggt aatggtgatc accacgtaac cattgccgac cagcgttttc aggtcggcat
    13681 tttctggaat ttcgccctgc acgcgcgcca caccgcgcat ttgctggtta ttgttaccgt
    13741 taataaccgc cagattcatc ggaccgtcgc cttgcagctg tacggtgata tcaccatcaa
    13801 acttcagcgt agcggttaac aggctggtcg caaccagcag ttctgccagc acgtttttaa
    13861 cgggctgcgg ataatcgtga ttctcaagga tctgttgcag ggtttccgaa acggttacca
    13921 gttcgccgcg cacggcaaag ttttcaaaca gatagcgatg taattggtca tgttgcggca
    13981 taatcatctc tcttgcaggt gacagttatt cactgtcgcc gtgtttaaat cgtaacaggt
    14041 cgcggcgctc ttttttgtcc gggcgtcggt ccgggtgcgg catggttaag gcattaagtt
    14101 tacgtgccag cgccattttt tcgcgtttct ctacactttc cgcagtctct tcatacagca
    14161 aggctgcctc gctggcgggg cgacgctgtt cagtaatcgc ctttacaatc accgtgcgtt
    14221 cgtcatttcc ctggcgcaga gtgagcgtgg cattcagctc gacgattttg ctcggcttgc
    14281 tgcgctgccc gttgtaatgc accttaccgc cttcaatcat ttcacgggcc agcgcgcggg
    14341 ttttataaaa acgggcagcc catagccatt tatccagtcg aacctcaaca gcaggttttt
    14401 ctttcatggc gtctccttca cattagcgag gggatcaggc ggcggtagtc attcagtgac
    14461 ggatggcgtt gatactgttt ctcggcaatc ccggaatcag gattagtcac gccgaggcag
    14521 taacgaatac caaattgcgc ggcagcatcg agaatcgctt cgctgtcatc aataaacagc
    14581 gttctttcag ctttcagacc cgtagcttcg gccaccgcat gccataaccg ctgatcctct
    14641 ttcggataac caaatgtgtg ggtggaaagt aataaatcaa ggtgtgcgtc cagaccggta
    14701 tgctcaagtt ttaccgccag gttgtgcgga tgcgcattgg tgagcaaaat tcgctgctta
    14761 ccgctggctt tcagtgcctc aagaaacgga atggtatctt cacgcagtac ggcacgcggt
    14821 cccatctcgg tggtcatcgc acagatatcc agacccagtt gctcactcca gtaatcaaga
    14881 cagtaccagt ttagcgtatg ctgtacgtcg tgatattgct ggcgcatata ttccatcgct
    14941 tcctgtggcg taaccccgtt tttcgcgccc catgtttcag gcaccagctt ttgccagaaa
    15001 tagttatcga aggcgaggtc gagcaacgtg ccgtccatat ccagcagaac ggtatctacg
    15061 tcctgccagg caatgttgat atgcatgagg gaaatctcca gagtgaagca atttgcgcga
    15121 cagggtagca taacctgccg cgcaaacgtg ttattcgata aggctttctg aaggggtgat
    15181 cagttgcggg ttcaggcagc tttcataata ggcctgaatc ttcatcatgc gagtgcgatg
    15241 acgctggtaa cgacgccagg cctgtacgcc attgtaaatg gcgctaccca gcatcgtcag
    15301 tagcagcaat gtggtgctga gatagcgcca caggccggaa cgatccggga ttggatgaag
    15361 gccaatatgc tgcgtaccat tggcgtcagt gaagattttt gtgacgatac cctcggcgtt
    15421 aaacggcgta tgcatcagca tttgtgccag tttctggaaa gcgttccact gttcttgcgg
    15481 cgggtagtcg taaagtgatg ccgaaggcca gggctgatca acaaaatcgc tgccttcgtc
    15541 gctgacaatc aggaatccgc cgggggccgg actgtttagt gattgtgccg ctcgcgccgt
    15601 ttcatgcgtg ataaacggcg cggtggaggt tgccaccagg ttatccagcg attccgcact
    15661 caccgggcgt aataacacat tcacgccatc gagcttcccg gcgttggcgc gttttaccag
    15721 cgcgtcccag tctttactgt tgccgagatt gaccagcgca tttttcagtc gcacacagtc
    15781 atctttggca gaacataaat ccgctgtctt cagtacaatg tcgccaaaat catcaagcaa
    15841 taccatgccg gatttttgaa ttgctgaacg taatgaggca ctgacgcgag atccatcttc
    15901 cggtttaggg tgcagctggc gattaactgc ttcagtcaat gccgtcgctt tgttgaccag
    15961 ttcagattct ggtaatggca atgagcgggc gtcgttccag atgatctgcg agcagtcaaa
    16021 cggtaaaaaa ggtgaattgg ttttcgcgct ccaggttccg gaagtgcgaa tattacacat
    16081 tcccgtaccg ctgatacgca atgtatcgcc tacccgcacg ccagcgtcag ccagttgttt
    16141 tacgctggtg gcttcaatgg tctgtgcgcc tttcatccat gagagagtga atttcagcgg
    16201 catatccagc gggatccaga ataacagcat aaacagcacc agcagcgagc ccgcagcgat
    16261 aatcgtactg cgcagccagt gctgtaacgg aaagtttttt acttcatcat gcagcgaaag
    16321 atatcgcccc tgacgcacta cgtggcggtc gagatagata tcaatatcgg tttgttgacc
    16381 gagatcctga gcaatgtatg gctgccagtg tgcgggatag accaggtcga taataccgag
    16441 tgaaatattg ttgatctgtt cctgatcgtt ttcgccaaac aatccccaac gacggggtgt
    16501 accgcgcaga caatgaattt cccgcaggga ggattttgcc gggggcgcga atagccccca
    16561 cagacctgcg cccagcagca gtaatgcgcc gcctgccagc cacggaacaa atacatccgg
    16621 ggtaatcagg cagaaaaaga acatcaggaa ggaggcgacg atcagcaacg cttcacgcag
    16681 cccgcgcgga cgactcagcg catattcttc atgcgtttct ttgcgaatat tgagtagctc
    16741 gatttgctca ctctcttccc cgcgaatcga cgcctgcgtg gacggaacag gttgcaaggc
    16801 atagccgcgc gtttcctgca tgtactcctg cagcgtatga ccgttgagtg aaataaccag
    16861 cggcagcgaa tcggtataaa tcagttcaac ggtattctca tcgttgatgt attgttccca
    16921 gaaagggggc aggtggactt ctaccgaatc gaggtagtaa cgccatttat tcggatcgtc
    16981 ggtagagatg ccgtaacgcg tgatagcgtg tgtcagcatc atgacgttgt tgctttcggc
    17041 attcagcgcc agagagatcg gtgccgcgct agctcccgtt gggccaggca cctgtaatac
    17101 ctgcgtcagg ctctcaagat aattttcaac ggcgctacgt tcttcaggtg tgagtttacg
    17161 cgtttgcgca tccgcgaagg cgttggtcca gggcagctga cgccgtctgg atcgcacttt
    17221 tatcagccat cccgcaagta gtgagcaggc cagcaaagca gctaaaaaaa tcacaatggt
    17281 gctcatgctt tccccatctt acttagtctg tcaggcgtgg tcagtcgcac cagacacaat
    17341 acatgattct gcggttggct atcggcaagc gtggagttta acttgagcat cattaagcac
    17401 atcacagtaa ccgctgatca tagcaaaccc tctgaaggcg ggatatcagc aaattcctgg
    17461 agttatttca acctattgac ttttaatttg atttgaggac gattatttat taagttacat
    17521 aaatgtaaat aacatgcaat tcacattgcc gaaagcgtgt ccgatatcgc acaataaccg
    17581 ctgatttcac agcatagatc ccacgatgag caaatcatta caaaaaccca ccattctgaa
    17641 tgttgaaact gtagcccatt cccgactgtt taccgtcgag agcgtggatc tggagttcag
    17701 caatggcgtg cggcgtgttt atgaacgaat gcgtccaacc aaccgggaag cagtgatgat
    17761 tgtgccgatt gtggacgatc acctgatcct gatccgcgaa tacgcagtgg gaactgaatc
    17821 ctacgaatta ggtttttcga aaggattaat tgatccgggt gaaagcgtct acgaagccgc
    17881 taaccgcgag ctaaaagaag aggttggatt tggtgcgaac gatctgactt ttttgaagaa
    17941 gctcagcatg gcaccgtctt acttttccag caaaatgaat atcgtggtcg cgcaagatct
    18001 ctacccggaa tcgctggaag gcgatgagcc agagccgcta ccacaggtgc gctggccgct
    18061 ggcgcatatg atggatttgc tggaagaccc tgacttcaac gaagcgcgta atgtcagcgc
    18121 gctgttcctc gtgcgcgaat ggttgaaagg gcaggggcga gtgtaaatgc gtgaagtttg
    18181 cctgatgcac aacgattatc aggcttatgg ggttcctgca atatattgat ttttcacgat
    18241 tttgttagcc ggataacgcg ttcacccgca tccggcataa acaaatgcac tttgtcagcc
    18301 aactgaaaag gcgccgaagc gcctttttaa tcagaacaat tcctgtgcct caccattatc
    18361 gataatggtt gttcccacct cgtgcaccgc ctgttgtgtc ggctgcgtac cttcgatgaa
    18421 atactcttcg cggctgttgc caccattagc taactgcccg gtgctgcgat cgatattcac
    18481 cgtcacaata cccggaggcg gcgtcagcgg ctgctccggc acaccttcaa gaacggcttt
    18541 catataagcg tcccatgcag gctgggcact cttggcaccg ccttcgtaac ctgagatctg
    18601 atctttaatc gctccggaag ccgttgtatg accgagatta cgacggtgat catcaaagcc
    18661 aatccagacc gaggtcacaa cgcccggacc gtaacccgag aaccacgcat ctttcgaact
    18721 gttagtggta ccggttttcc cgccgatatc gcgacgctgc aaatcacgac ctgcacgcca
    18781 gccggtaccc tgccagcctg gttcaccaaa gatattggtg ttcaaagcac tcttaatcag
    18841 gaatgccagc ggagtgttga tgacgtgcgg tgcgtactcc tgcgcgccag tcttcgccac
    18901 taacgcctga tttgcctgct ccagctgcgg cattggtaca gaaacattct gctgctcgcg
    18961 ggagatagcg acatcttcaa catcgttatt ttccagcacg ttcgatttct gcgtatcacc
    19021 gtaaatcacc ggaatatcgc attccgggca ggctactttc ggtttcgctt cgaaaatcac
    19081 gccgccctga tcgttttcaa ttttgctgat aaaccacggg tccaccagga agccgccgtt
    19141 cgccatgacc gcgtagccgc gcgccacctg cattggggtg aaggacgctg aacccagcgc
    19201 cagcgattcg gtgtggacaa tgttttgcgc cgggaagccg aagcgttgca gatattctgc
    19261 agcgtagtcg acgcccatcg cccgcattgc acgtaccatc accacgtttt tcgactgacc
    19321 cagcccctga cgtaagcgaa ttggaccagc atactgcggt ggtgagttct tcggctgcca
    19381 gtcagaaccg acacctgcat cccagcgaga aattggcaca tcgttcaaca tacttgccag
    19441 cgtcagacct ttatccatcg ccgcggtgta gaggaacggt ttgatgttgg aacccacctg
    19501 acgcagtgcc tgggtggcgc ggttaaactt gctctgattg aaatcaaagc caccgaccag
    19561 cgccataacg gcaccatttt gcggattgat cgacaccagc gccgagttca cttccggcac
    19621 ttgtgccagc caccatgcat cgccaacctg acgaacccag atttgctgac ccgtttgcag
    19681 aacatcggtc actttacgcg gcgtcggtcc ctgctgagta tccgaacggt aaggacgcgc
    19741 ccagcgaacg ccgtccatac tcaatgcgac agtcgacccg tccgccagca ttgccgtcgc
    19801 ttcctgagga ttggcgctgg tgactgcggc aggcagcagc ggaccatagg tcggcagcgc
    19861 cttcagcgta tcggtaatct tgttgttatc ccacgccgac tcgcccactt tccacagcac
    19921 atttgccggg ccgcgatagc cgtggcgcat gtcgtagtcc agcacgttat tacgtaccgc
    19981 ctgctgtgcg gcctgctgca ctttgcgggt gatggtggtg taaatgcgat aaccgtcttc
    20041 ataggcactt tcgccataac ggttatacat ctcctggcgc accatttcgc tcaggtacgg
    20101 cgcagagaaa gcaatctccg gcgcgtgata gttagcgtta atcgcctcag tgcgtgtctg
    20161 atcgaactgt tgttgggtga tatacccttc atccagcatc cgcgacagta cgacgttacg
    20221 ccgcgcgacg gcacgatcca ttgagtagag cggattaaag gtggaaggcg ctttcggcag
    20281 cccggctatc acagccattt cgttcagcgt cagttggtcg accgttttac cgaaatagac
    20341 ttgtgccgca gcaccgacac cataggcgcg gtaaccgagg taaatcttgt tcagataaag
    20401 ctcgaggatc tcgtctttcg tcagcaactg ttcaatgcga atcgcgagga agacttcctt
    20461 aatcttacgc atcagcgtgc gttctggact gaggaagaag tttctcgcca gctgctgggt
    20521 aatggtactc gccccttgtg acgcgtggcc ggagaacagc gccacacttg ccgcacggaa
    20581 gatccccacc gggtcaacgc cgtgatgctc gtagaagcgg ctgtcttccg tcgcgataaa
    20641 ggctttcacc atctccggcg ggatttgatc caacgtaacc ggaatacgac gtttctcacc
    20701 gtattgagca atcagctcgc catcggcgct gtaaatctgc atcggaattt gcaggcgaac
    20761 atcttttaat gtcgccacat ccggcagttg tggctcgatg tagcggtata ggccataaat
    20821 cgagcctgct cccagcagaa tgcaacagac tgcaaggatc aaaaaatact ttacgaactt
    20881 cactggaaat ttcccattta gtttcatttg ggcagtttat aaacaaacgt gcggtagtat
    20941 aaaggcaagc cagacgcatt gatatacccg tcagagtgac gggtgataag gagatcatca
    21001 caatggcatt taagatctgg caaattggtt tgcatttaca acagcaagaa gcagtagcgg
    21061 ttgcgatcgt gcggggcgca aaagaatgct ttttgcaacg ctggtggcgg ttgccgctgg
    21121 agaacgacat tatcaaagat gggcggattg ttgatgcgca gcggctggct aaaacgttgt
    21181 taccgtggag tcgcgaactg ccgcagcgtc atcacattat gttggcgttt cccgccagtc
    21241 gcacattaca gcggtcattt ccgcgcccgt cgatgtccct cggtgagcgg gagcaaacgg
    21301 cctggctgtc agggacgatg gcccgcgagc tggatatgga tccggactcc ctgcgtttcg
    21361 attacagcga agactcactc agtcccgcct ataacgtgac tgccgcgcaa agcaaagagc
    21421 tggcaacgct actcactctg gcagaaaggt tgcgtgttca tgtgagcgcg atcaccccgg
    21481 atgccagtgc attacagcga ttcctgcctt ttttaccttc tcatcagcaa tgtctggcct
    21541 ggcgtgataa cgaacagtgg ctgtgggcga cacgctgtcg ctgggggcgc aaactggcgg
    21601 tggggatgac tagcgcgaag gagctggcgg cagcgttatc cgttgatccc gaaagcgtcg
    21661 cgatatgtgg cgaaggcgga tttgatccct gggaggccgt ttctgttcgt cagccgccgc
    21721 taccgccgcc aggtggagac tttgccatcg cgctggggct ggcgcttggg aaggcgtact
    21781 gatgaacccg ccaattaatt ttttgccctg gcgacagcaa cgccggaccg cttttcttcg
    21841 tttctggttg ctgatgttcg ttgcgcctct gctgctggcc gtcgggataa cgctaatact
    21901 gcgtctgaca gacaacgccg aagcacgcgt aaacaccgtt ttgcttcagg cggaacaaca
    21961 actcgcccgc agcttacaga taacgaaacc acgtttgctg gagcgccaac aaattcgtga
    22021 gcagcgtttg caacggcagc gccagcgaca atttacccgc gactggcaat ctgcgctgga
    22081 agcactggcg gcgcttttac ccgagcacgc ctagctgaca acgataagct ggcagcaggg
    22141 aacgctggag atcaaggggc tgacaacaag cattaccgcg ttaaacgcac tagaaacgtc
    22201 actccgccag gatgcgtctt ttcatctcaa tcagcgggga gccacgcagc aggatgcgca
    22261 gggacgctgg caatttgagt atcagttaac aaggaaggtt agcgatgaac atgttctttg
    22321 actggtggtt cgccacatca ccccgcctcc gccagttttg ctgggcattc tggttgctga
    22381 tgttagttac gctcattttt ctgtcatcga cacaccatga agagcgcgac gcattaattc
    22441 gactacgggc aagtcatcac cagcagtggg ccacactgta tcgcctggtt gacaccactc
    22501 ccttcagcga agaaaaaacg ctgccctttt cgccactgga ttttcagttg cccggcgcgc
    22561 aactggtttc ctggcatcca tccgcgcagg gaggcgagtt ggcgttgaaa acgctgtggg
    22621 aagcagtgcc gtcggcattt acacggctgg cagagcgcaa cgtcagcgtg agccgttttt
    22681 cattaagcgt ggaaggtgat gatcttttgt tcacgctaca actggagacg ccgcatgagg
    22741 gttaaacgct ggttgttggc aggtattgta ttgtgccttt taaccggtat gcgtgaccct
    22801 tttaaaccgc cggaagatct atgccggatt agcgaactta gccagtggcg ctatcagggg
    22861 atggtagggc gaggcgagcg catcatcggt gtaataaaag acgggcaaaa gaaatggcga
    22921 cgggtgcagc aaaacgatgt gctggaaaac ggctggacaa ttttacagct gacgccagac
    22981 gtactaacgc tgggtaccgg gacaaactgc gaaccgccac aatggttgtg gcaacggcaa
    23041 ggagatacaa atgaagcaat ggatagccgc actactgttg atgctgatac ccggcgtaca
    23101 ggcggcaaag ccgcaaaaag tgacgctgat ggtggatgac gttccggtag ctcaggtgtt
    23161 gcaggcgctg gctgaacagg agaagttgaa cctggtcgtg tcgccagacg tcagcggtac
    23221 ggtgtcgtta catctaacag atgttccctg gaagcaggca ctacaaactg tagtgaaaag
    23281 cgccggactg ataacgcggc aggaaggcaa cattctctca gtgcattcca ttgcctggca
    23341 gaataacaat atcgcccgcc aggaggcgga gcaggcgcgg gcgcaggcaa atctgccgct
    23401 ggaaaatcgc agtataaccc tgcaatacgc cgacgcggga gaactggcga aagcggggga
    23461 gaagctactg agtgccaaag ggagtatgac cgtcgataaa cgcaccaatc gccttttgct
    23521 acgagataac aaaacggcgt taagcgcgct tgaacagtgg gtagcgcaaa tggatctgcc
    23581 ggtcgggcag gttgagctgt cggcgcatat tgtcaccatt aatgaaaaaa gtttgcgtga
    23641 gttaggcgtg aaatggacgc tggccgatgc gcaacacgct ggtggcgttg ggcaagtcac
    23701 cacgcttggt agcgacctct ccgtagcgac ggcgacaacg catgtcggtt ttaacattgg
    23761 gcgcatcaac ggacgcttgc tggatcttga gctttccgcg ctcgaacaaa aacagcagct
    23821 ggatattatc gccagtccgc gtctgctggc ctcacatctt cagcctgcca gcattaaaca
    23881 ggggagcgaa attccatatc aggtttccag cggggaaagt ggcgcgacgt cggtggaatt
    23941 taaagaggcc gtcctgggga tggaagttac gcccacagtg ttacaaaaag gtcgcatccg
    24001 gctgaaatta cacatcagcc agaacgttcc ggggcaggtg ctacagcagg ccgatggcga
    24061 agtgctggcg attgataagc aggagatcga aacgcaggtc gaggtcaaaa gcggagaaac
    24121 gttggcgctg ggcggcattt ttacccgtaa aaataaatcg ggtcaggata gcgtaccgtt
    24181 gcttggcgac attccctggt tcgggcaatt atttcgtcat gacggaaaag aagatgaacg
    24241 acgcgagtta gtggtgttta tcacgccacg actggtttcc agtgagtaaa cagccgtaaa
    24301 agcggtaatg tttttacgct gaacgtgttt catctatttg acgcgcgcag gtatttagca
    24361 tacaaggagt accgatttga gagttggtgc tcttcgctgc ctgcgttcca tgatgatgat
    24421 ttatcattca ggcggcattt tgctgtcttt tttacgctaa tcttacccgg tgatttatcg
    24481 ccagagcggt ggtagcaagg cagcgcgctt gcagcgacca gatatgcaga gggatgggtg
    24541 atttattcag ttgccaaacc cgctggagta ttgagataat tttcagtctg actctcgcaa
    24601 tatcttatga ggtttcagtt catgtcctgc ggcgctctct gagcgaagcg ggtttatcat
    24661 taacgaatag tcttagtagt accgaaaaaa tggcagagaa acgcaatatc tttctggttg
    24721 ggcctatggg tgccggaaaa agcactattg ggcgccagtt agctcaacaa ctcaatatgg
    24781 aattttacga ttccgatcaa gagattgaga aacgaaccgg agctgatgtg ggctgggttt
    24841 tcgatttaga aggcgaagaa ggcttccgcg atcgcgaaga aaaggtcatc aatgagttga
    24901 ccgagaaaca gggtattgtg ctggctactg gcggtggctc tgtgaaatcc cgtgaaacgc
    24961 gtaaccgtct ttccgctcgt ggcgttgtcg tttatcttga aacgaccatc gaaaagcaac
    25021 ttgcacgcac gcagcgtgat aaaaaacgcc cgttgctgca cgttgaaaca ccgccacgtg
    25081 aagttctgga agcgttggcc aatgaacgca atccgctgta tgaagagatt gccgacgtga
    25141 ccattcgtac tgatgatcaa agcgctaaag tggttgcaaa ccagattatt cacatgctgg
    25201 aaagcaacta attctggctt tatatacact cgtctgcggg tacagtaatt aaggtggatg
    25261 tcgcgttatg gagaggattg ttgttactct cggggaacgt agttacccaa ttaccatcgc
    25321 atctggtttg tttaatgaac cagcttcatt cttaccgctg aaatcgggcg agcaggtcat
    25381 gttggtcacc aacgaaaccc tggctcctct gtatctcgat aaggtccgcg gcgtacttga
    25441 acaggcgggt gttaatgtcg atagcgttat cctccctgac ggcgagcagt ataaaagcct
    25501 ggctgtactc gataccgtct ttacggcgtt gttacaaaag ccgcatggtc gcgatactac
    25561 gctggtggcg cttggcggcg gcgtagtggg cgatctgacc ggcttcgcgg cggcgagtta
    25621 tcagcgcggt gttcgtttca ttcaagtccc gacgacgtta ctgtcgcagg tcgattcctc
    25681 cgttggcggc aaaactgcgg tcaaccatcc cctcggtaaa aacatgattg gcgcgttcta
    25741 ccagcctgct tcagtggtgg tggatctcga ctgtctgaaa acgcttcccc cgcgtgagtt
    25801 agcgtcgggg ctggcagaag tcatcaaata cggcattatt cttgacggtg cgttttttaa
    25861 ctggctggaa gagaatctgg atgcgttgtt gcgtctggac ggtccggcaa tggcgtactg
    25921 tattcgccgt tgttgtgaac tgaaggcaga agttgtcgcc gccgacgagc gcgaaaccgg
    25981 gttacgtgct ttactgaatc tgggacacac ctttggtcat gccattgaag ctgaaatggg
    26041 gtatggcaat tggttacatg gtgaagcggt cgctgcgggt atggtgatgg cggcgcggac
    26101 gtcggaacgt ctcgggcagt ttagttctgc cgaaacgcag cgtattataa ccctgctcaa
    26161 gcgggctggg ttaccggtca atgggccgcg cgaaatgtcc gcgcaggcgt atttaccgca
    26221 tatgctgcgt gacaagaaag tccttgcggg agagatacgc ttaattcttc cgttggcaat
    26281 tggtaagagt gaagttcgca gcggcgtttc gcacgagctt gttcttaacg ccattgccga
    26341 ttgtcaatca gcgtaacaac aagaaaggtc aggccgctta tcaagcggtc tattagcttc
    26401 aggttaattg caacgtggta agcattaacc ttttagtggg gtgttaaatg gatgaattca
    26461 aaccagaaga cgagctgaaa cccgatccca gcgatcgtcg tactggtcgt tctcgtcaat
    26521 cttctgaacg ttctgagcgt actgaacgtg gcgaaccgca gatcaatttt gatgatattg
    26581 aacttgatga cactgacgat cgccgtccga ctcgtgcgca aaaagagcgc aatgaggaac
    26641 cggaaatcga agaagaaatt gacgaatccg aagatgaaac cgtggatgaa gagcgcgtag
    26701 agcgtcgtcc gcgtaagcgc aaaaaagcag ccagtaaacc cgcttctcgt cagtatatga
    26761 tgatgggcgt cggcattctg gttctactgc tgttgatcat cggtatcggt tctgcgctaa
    26821 aagccccctc gacctcttcc agcgatcaaa ccgcgtctgg cgagaagagt attgatcttg
    26881 caggcaatgc gaccgatcag gcgaatgggg tgcagccagc gccgggaacc acgtctgcgg
    26941 aaaatactca gcaggatgtt tctctgccac cgatctcttc tacgccgact caagggcaaa
    27001 ccccggcggc aacggatggt caacaacgtg ttgaagtgca gggtgacctg aacaatgcgc
    27061 tgacccagcc acaaaatcag caacagttga acaatgtggc ggtcaattcc acattgccga
    27121 ccgaacccgc gactgtcgcg cctgttcgca atggcaatgc atcgcgtgac acggcgaaaa
    27181 cgcaaaccgc tgaacgtccg tccactacgc gcccagctcg tcagcaggcg gtgattgaac
    27241 cgaaaaaacc gcaagcaacc gtgaaaacgg agccgaagcc ggtagcacag acgccgaagc
    27301 gtactgaacc agctgcccct gtggcgagca cgaaggcacc gactgcgact tctacgccag
    27361 caccaaaaga gacggcgact acggctccag tacagacggc atccccggcg caaaccacgg
    27421 caacaccagc cgctggaggg aagaccgcag gtaatgttgg ttcgttgaaa tcggcaccgt
    27481 ccagccatta cactctgcag ctgagcagtt cctctaacta cgacaacctg aacggttggg
    27541 cgaagaaaga gaatctgaaa aactacgttg tctatgaaac ggcgcgtaat ggtcagccgt
    27601 ggtatgtcct ggtttctggc gtgtacgctt cgaaagaaga ggcgaaaaaa gcggtatcta
    27661 cattgccagc agatgttcag gccaaaaacc cgtgggcgaa accgctgcgt caggtacagg
    27721 ccgatctgaa gtaatcaagg ttatctcccg caatggttta tcgttgcggg agctgcctga
    27781 agcgctggat gctgtcggag ctttctccac agccggagaa ggtgtaatta gttagtcagc
    27841 atgaagaaaa atcgcgcttt tttgaagtgg gcagggggca agtatcccct gcttgatgat
    27901 attaaacggc atttgcccaa gggcgaatgt ctggttgagc cttttgtagg tgccgggtcg
    27961 gtgtttctca acaccgactt ttctcgttat atccttgccg atatcaatag cgacctgatc
    28021 agtctctata acattgtgaa gatgcgtact gatgagtacg tacaggccgc acgcgagctg
    28081 tttgttcccg aaacaaattg cgccgaggtt tactatcagt tccgcgaaga gttcaacaaa
    28141 agccaggatc cgttccgtcg ggcggtactg tttttatatt tgaaccgcta cggttacaac
    28201 ggcctgtgtc gttacaatct gcgcggtgag tttaacgtgc cgtttggccg ctacaaaaaa
    28261 acctatttcc cggaagcaga gttgtatcac ttcgctgaaa aagcgcagaa tgcctttttc
    28321 tattgtgagt cttacgccga tagcatggcg cgcgcagatg atgcatccgt cgtctattgc
    28381 gatccgcctt atgcaccgct gtctgcgacc gccaacttta cggcgtatca cacaaacagt
    28441 tttacgcttg aacaacaagc gcatctggcg gagatcgccg aaggtctggt tgagcgccat
    28501 attccagtgc tgatctccaa tcacgatacg atgttaacgc gtgagtggta tcagcgcgca
    28561 aaattgcatg tcgtcaaagt tcgacgcagt ataagcagca acggcggcac acgtaaaaag
    28621 gtggacgaac tgctggcttt gtacaaacca ggagtcgttt cacccgcgaa aaaataattc
    28681 tcaaggagaa gcggatgaaa cagtatttga ttgccccctc aattctgtcg gctgattttg
    28741 cccgcctggg tgaagatacc gcaaaagccc tggcagctgg cgctgatgtc gtgcattttg
    28801 acgtcatgga taaccactat gttcccaatc tgacgattgg gccaatggtg ctgaaatcct
    28861 tgcgtaacta tggcattacc gcccctatcg acgtacatct gatggtgaaa cccgtcgatc
    28921 gcattgtgcc tgatttcgct gccgctggtg ccagcatcat tacctttcat ccagaagcct
    28981 ccgagcatgt tgaccgcacg ctgcaactga ttaaagaaaa tggctgtaaa gcgggtctgg
    29041 tatttaaccc ggcgacacct ctgagctatc tggattacgt gatggataag ctggatgtga
    29101 tcctgctgat gtccgtcaat cctggtttcg gcggtcagtc tttcattcct caaacactgg
    29161 ataaactgcg cgaagtacgt cgccgtatcg acgagtctgg ctttgacatt cgtctggaag
    29221 tggacggtgg cgtgaaggtg aacaacattg gcgaaatcgc tgcggcgggc gcggatatgt
    29281 tcgtcgccgg ttcggcaatc ttcgaccagc cagactacaa aaaagtcatt gatgaaatgc
    29341 gcagtgaact ggcaaaggta agtcatgaat aagtttgaag atattcgcgg cgtcgctttt
    29401 gatcttgatg gtacgctggt cgacagtgct cccggtcttg ctgctgcggt agatatggcg
    29461 ctgtatgcgc tggagttgcc catcgcaggt gaagaacgcg ttattacctg gattggtaac
    29521 ggcgcagatg ttctgatgga gcgcgcattg acctgggcgc gtcaggaacg tgcgactcag
    29581 cgtaaaacaa tgggtaaacc gcccgttgat gacgacattc cggcagaaga acaggtacgt
    29641 attctgcgta aactgttcga tcgctactat ggcgaggttg ccgaagaggg gacgtttttg
    29701 ttcccgcacg ttgccgatac gttgggcgcg ttgcaggcta aaggcctgcc gctaggcctg
    29761 gtcaccaaca aaccgacgcc gttcgtcgca ccgctgctcg aagccttaga tattgccaaa
    29821 tactttagcg tggttatcgg cggcgatgat gtgcaaaaca aaaaaccgca tccggacccg
    29881 ctgttactgg tggctgagcg gatgggaatt gccccacaac agatgctgtt tgtcggcgac
    29941 tcacgcaatg atattcaggc ggcaaaagcg gcaggttgcc catcagttgg cttaacctac
    30001 ggatataact acggcgaggc tatcgatctc agccagcctg atgtaattta tcagtctata
    30061 aatgaccttc tgcccgcatt agggcttccg catagcgaaa atcaggaatc gaaaaatgac
    30121 taagcccatc gtttttagtg gcgcacagcc ctcaggtgaa ttgaccattg gtaactacat
    30181 gggtgcgctg cgtcagtggg taaacatgca ggatgactac cattgcattt actgtatcgt
    30241 tgaccaacac gcgatcaccg tgcgccagga tgcacagaag ctgcgtaaag cgacgctgga
    30301 tacgctggcc ttgtatctgg cttgtggtat cgatcctgag aaaagcacca tttttgttca
    30361 gtcccacgta ccagaacatg cgcagttagg ctgggcactg aactgttata cctacttcgg
    30421 cgaactgagt cgcatgacgc agtttaaaga taaatctgcg cgttatgccg agaacatcaa
    30481 cgctggtctg tttgactatc cggtgctgat ggctgcggac atcctgctgt atcaaactaa
    30541 tctggtaccg gtgggtgaag accagaaaca gcacctggaa ctgagtcgcg atatcgccca
    30601 gcgtttcaac gcgctgtatg gcgagatctt taaggtgccg gagccgttta ttccgaaatc
    30661 tggcgcgcgc gtaatgtcgc tgctggagcc gaccaagaag atgtccaagt ctgacgataa
    30721 ccgcaataac gttatcggcc tgctggaaga tccgaaatcg gtagtgaaga aaatcaaacg
    30781 cgcggtcact gactccgacg agccgccggt agttcgctac gatgtgcaga acaaagcggg
    30841 cgtttccaac ctgctggata tcctttctgc ggtaacgggc cagagcatcc cagaactgga
    30901 aaaacagttc gaaggcaaga tgtatggtca tctgaaaggt gaagtggctg atgccgtttc
    30961 cggtatgctg actgaattgc aggaacgcta tcaccgtttc cgcaacgatg aagccttcct
    31021 gcaacaggtg atgaaagacg gcgcagaaaa agccagtgta cacgcttccc gtacgctgaa
    31081 agcggtgtac gaagcgattg gttttgtggc gaagccgtaa tttaacatta atgccaaaaa
    31141 ccggggaaac ccggtttttt tatcctcatt tgcaataatc cgaaaaaatg tgaagcgcct
    31201 cgccgtttcc acatctcgtc gttgccactt atcttcattt taatgaagat ataaatattc
    31261 attttattga aaatatatta tgcgtcgaac gtttatcaaa aaagaaggcg tcgtcatcac
    31321 gacgctggcc cgttatttgt tgggtgaaaa atgcggtaat cgactgaaaa ccatagatga
    31381 gctggcaact gaatgccgtt catcggttgg cctgacgcag gccgcgttga aaacgttgga
    31441 atcaagcgga gcgatacgga ttgaacgccg tgggcgcaat ggcagttatc tggtcgagat
    31501 ggataacaaa gcattgctga gtcatgtgga tatcaacaac gtggtatgtg cgatgccttt
    31561 gccctatacc cgcttgtacg aaggtttagc gagcgggctg aaagctcagt ttgatggcat
    31621 tcctttttac tatgcgcata tgcgcggcgc ggatattcgc gtggagtgtt tacttaacgg
    31681 cgtgtatgac atggcggtgg tttcccgact ggcggcggaa agttatctca cgcaaaaagg
    31741 gttatgcctc gcgctggagt tggggccgca cacctacgtt ggcgagcacc agttgatttg
    31801 ccgtaaaggc gagtccgcaa acgtgaagcg cgtggggctg gataaccgtt cggcggatca
    31861 gaaaatcatg accgatgtct tttttggcga tagtgatgtg gaacgtgtcg atctctctta
    31921 tcacgagagt ttacaacgca ttgttaaagg cgatgtcgat gcggttatct ggaacgtggt
    31981 ggcggaaaac gaactgacca tgctgggatt agaggcgacg ccgctcacag acgatccgcg
    32041 atttttacag gccaccgaag ccgtggtcct gacgcgagtc gatgattacc caatgcaaca
    32101 actgctgcgt gccgttgtag ataaacacgc cctgctcgcc catcaacaac gggtagtgag
    32161 tggggaacag gaaccgagtt attaaacgaa aggcatctga tatggaaacc agactcaacc
    32221 tgctttgcga ggcaggcgtt attgataagg acatctgcaa aggcatgatg caggtcgtca
    32281 acgtactgga aacagagtgc catctgccgg tgcgcagtga gcaaggaacg atggcgatga
    32341 cacatatggc gagtgcactg atgcgcagtc gccgtggtga agaaatagag ccgctggata
    32401 acgaattgct ggcagagctg gcgcaatcca gccactggca agccgttgtg caattgcatc
    32461 aggtgttgtt gaaggaattc gcactggaag ttaacccgtg tgaagaaggc tatttgctgg
    32521 ccaaccttta tggattatgg atggctgcta acgaagaggt ttgaagctcc cgacaaatcc
    32581 acgtcgcaca acctaaatat tcaaaaaaat gaatatttaa ttcaaggaac agaaacgagg
    32641 caacaagatg tttgtagaag cattgaaacg ccagaacccg gcgctgattt ccgccgcact
    32701 aagtttgtgg cagcagggca agatcgcccc ggacagctgg gtgatcgacg tggatcaggt
    32761 actggaaaac ggtaagcgac tgattgagac ggcgcggctt tacggcattg aactgtatct
    32821 gatgaccaag cagtttggcc gtaacccatg gctggcggaa aaattgctgg cattaggtta
    32881 cagcggcatt gtggcggtgg attacaaaga ggcgcgagtc atgcgccgcg ctggtttgtc
    32941 ggtggcacat caggggcatc tggtacaaat cccttgtcat caggttgctg acgccgttga
    33001 acagggcacc gacgtcatca ccgtgtttac tctcgacaaa gcgcgggaag tttctgcggc
    33061 agcggtgaag gccggacgtg tgcagtttgt gctgctcaaa gtttatagcg acgatgattt
    33121 tctttatccg gggcaggaga gcggttttgt acagcattcg ctgcacgagg tggttgctga
    33181 aattaagaaa ctgccggggc tgcatttagc cggacttacc catttccctt gcctgctttg
    33241 ggatgaggct gccggaaaag ttttgccgac accgaatctt cacacgctga tacaggcacg
    33301 ggatcaactg gcgaaatctg gtattgcact tgagcaactg aacgcgcctt cagcgaccag
    33361 ctgcacttcg ctgccattac tggcgtaata cggtgtgact catgccgaac ccggtcatgc
    33421 gctgacgggc actattccgg caaaccagca gggcgatcag tctgaacgta tcgcgatgct
    33481 ctggttgagt gaaatctccc atcatttccg tggcgacagc tactgctacg gcggcggtta
    33541 ctaccgtcgt ggtcatgcgc aacatgcgtt ggtgtttacg ccagaaaatc aaaagattac
    33601 tgaaaccaat ctcaaaactg tggatgacag cagtatcgac tacaccctgt cgctggcagg
    33661 cgagtttccg gtaagcagtg cagtggtgct ctgtttccgc acgcagattt ttgtcacccg
    33721 tagcgatgtg gtgctggtgt ccggtattca ccgtggcgaa ccgaaaatcg ttggtcgtta
    33781 tgacagtctt ggaaactctc tgggggcgta atggcgcgat ttgtggtgtt agtgattgat
    33841 agctttggcg taggggcaat gaaagatgtc acgctggtgc gtccgcaaga tgcgggagcg
    33901 aatacatgtg gtcacatcct gagccagttg ccgcatttgc agctaccaac gctggagaag
    33961 ctggggctaa tcaacgcatt gggttatgcg ccaggcgata tgcagccgtc agattccgca
    34021 acctggggcg tggcagagct gcaacatgaa ggtggcgata cctttatggg gcatcaggaa
    34081 attttaggca cgcgcccgtt accgccgctg cggatgcctt ttcgcgatgt gattgaccgt
    34141 gttgagcagg cattagtatc cgccggttgg caggtggagc gccgtggcga tgaactgtaa
    34201 tttctgtggg tcaatcaggc ggttgcgatt ggcgataatc tcgaggcgga tttaggccaa
    34261 gtctataaca ttaccgccaa tctctctgtg atctcttttg acgacgcaat caaaattggt
    34321 cgtatcgtgc gtgagcaggt acaggtcggt cgggtcatta catttggtgg cctgttaacc
    34381 gacagtcaac gcattctcga tgccgcagaa agcaaagaag ggcgctttat tggtatcaat
    34441 gcgccgcgtt ctggcgctta tgacaacggc ttccaggtcg tgcatatggg ctacggtgtc
    34501 gataaaaaag tgcaggtgcc gcaaaaactg tatgaggcgg gcgtgccaac cgtgctggtg
    34561 ggtaaggtgg cagatatcgt cagcaatcct tatggcgtga gctggcaaaa cctggtggat
    34621 agccagcgga ttatggatat caccctcgac gaatttaaca cccatccgac ggcgtttatt
    34681 tgcaccaata tccaggaaac cgacctcgct ggtcatgcag aagacgtcgc acgttatgcc
    34741 gaacgtttgc aggtcgttga ccgtaacctt gcccggcttg ttgatgcgat gcagccagat
    34801 gattgcctgg tcgtgatggc ggatcacggc aacgatccga ccattggtca cagccaccat
    34861 acccgcgaag tggtgccgct gctggtttat cagcaaggcc tggctgctac gcagctcggt
    34921 gtgcgcacca cgctttctga tgtgggagct accgtgtgtg aatttttccg tgcgccaccg
    34981 ccacaaaatg gtcgctcttt tctttcctcc ctccggtttg caggagacac cctatgagtt
    35041 ttgatccgac gggttacacc ctggcccatg agcatctgca tattgatctc tccggcttta
    35101 aaaacaacgt ggactgccgc cttgatcagt atgcgttcat ttgccaggag atgaacgacc
    35161 tgatgacccg gggtgtacgt aatgtgattg agatgaccaa ccgttacatg gggcgcaatg
    35221 cgcaatttat gctcggtgta atgcgcgaaa ccgggatcaa cgtggtggcc tgtactggtt
    35281 attaccagga tgcgtttttc ccggaacatg tggtgacccg cagcgtgcag gaactggcgc
    35341 aggagatggt cgatgaaatt gaacagggta tcgatggcac ggagctgaaa gccgggatca
    35401 tcgcggagat cggctccagc gaaggaaaga ttacgccgct ggaagagaag gtatttattg
    35461 ccgctgcgct ggcgcataac cagaccggac gcccgatctc cacgcatacg tcgttcagca
    35521 ccatggggct ggaacaactg gctctgttgc aagcccacgg agttgatctt tcgcgcgtca
    35581 ccgttggtca ctgcgatctg aaagacaacc tcgacaacat tttgaagatg atcgatctcg
    35641 gcgcgtacgt gcagttcgac accattggta agaacagtta ctacccggac gaaaagcgca
    35701 ttgcgatgct ccacgcgcta cgtgaccgtg ggttgctgaa ccgcgtcatg ctgtcgatgg
    35761 atattacgcg ccgctcccat ttaaaagcca atggtggtta tggctatgac tatttattga
    35821 ccacctttat tccgcaattg cgccagtcag gattcagtca ggccgatgtg gatgtgatgt
    35881 tacgtgaaac tccctctcaa tttttccaat aaggacagac tcatgaaaaa gattggcgtt
    35941 gcaggcttac agcgtgagca gattaaaaaa accattgaag cgacggctac tggcagtttt
    36001 gaagttttca ttcacaacga catggaagcg gcaatgaaag tgaaatccgg gcaactggat
    36061 tactacatcg gtgcgtgtaa taccggtgcg ggcgcggcat tgtcgattgc catcgcggtg
    36121 attggctata acaaaagttg caccattgcc aaaccaggca ttaaagcgaa agacgagcat
    36181 atcgccaaaa tgatcgctga aggaaaagtg gcgtttggcc tttccgttga gcacgtcgaa
    36241 cacgcgattc cgatgctgat taaccatctg aaataaaagg cacgactatg gatctgtata
    36301 ttcagattat cgtggtggcg tgcctgacgg gtatgacatc gcttctggcg catcgctcgg
    36361 cggctgtttt tcatgacggt atccgcccga tcctgccgca actgattgaa ggctatatga
    36421 accgtcgcga ggcggggagt atcgcttttg gtctgagcat tggttttgtg gcctcggtgg
    36481 ggatctcttt taccctgaaa accgggctgc tcaacgcatg gttactcttt cttcctaccg
    36541 atatcctcgg cgtactggcg ataaacagcc tgatggcgtt tggtcttggc gctatctggg
    36601 gcgtgttgat ccttacttgc ctgttgccag taaaccagct gctgaccgcg ctgccggtgg
    36661 atgtattagg tagcctcggg gaattaagct cgccggtggt ttctgctttt gcactgttcc
    36721 cgctggtggc gattttctac cagtttggct ggaagcaaag tctggtcgcc gccgtggttg
    36781 ttctgatgac ccgtgtggta gtcatgcgct atttcccaca tcttaaccct gaatccatcg
    36841 aaatctttat tggcatggtg atgctgctgg ggatcgcgat aactcacgac ctgcgtcatc
    36901 gtgatgaaaa tgatattgat gccagcgggc tttcggtgtt tgaagaacgc acgtcgcgga
    36961 ttatcaaaaa cttaccctat atcgccatcg tgggagcatt gattgccgcc gttgccagca
    37021 tgaagatttt cgccggcagt gaagtgtcga tcttcactct ggagaaagcg tactccgcag
    37081 gcgtaacgcc ggaacaatcg caaacgctga tcaaccaagc ggcactggca gaatttatgc
    37141 gcgggctggg ttttgtaccg ttgattgcca ccaccgcctt agcaacgggt gtgtatgcag
    37201 ttgcgggctt tacctttgtt tatgcggtgg gctatctctc gccgaatccg atagttgcag
    37261 cggtattagg cgcagtggtt atttcggcgg aagtcttgct gcttcgttcg atcggcaaat
    37321 ggctgggacg ctacccgtcg gtgcgtaatg cgtcggataa catccgtaac gccatgaata
    37381 tgctgatgga agtggcgctg ctggtcggtt cgattttcgc agcaattaag atggcgggtt
    37441 ataccggatt ctctatcgcg gttgccattt acttcctcaa cgaatccctg ggccgtccgg
    37501 tacagaaaat ggcggcaccg gtcgtggcag taatgatcac cggtattctg ctgaatgttc
    37561 tttactggct tggcctgttc gttccggctt aaggaaggca cctatgaaga cgtttcctct
    37621 gcaaagtctg acgattattg aggcgcagca aaagcagttt gcgctggtgg atagcatttg
    37681 tcgccatttc cccggcagcg agtttcttac tggcggtgat ttaggcttaa cgccaggact
    37741 gaatcaaccg cgcgttaccc agcgtgtgga acaggtgctg gctgatgcat ttcacgcaca
    37801 ggctgcggcg ctggtgcagg gtgcgggtac tggcgcgatt cgtgcggcgt tggcggcttt
    37861 gctcaaaccg gggcagcgtc ttctggtaca tgatgcgcct gtttacccga cgacacaggt
    37921 cattattgag cagatggggc tgacgcttat tactgctgat ttcaatgacc tgtcggcact
    37981 gaagcaggtc gtcgacgagc aactaccgga tgcggcgctg gtgcagcata cgcgccagca
    38041 gccgcaggac ggctacattc tggcagatgt gctggcaaca ttgcgctcgg ctggcgttcc
    38101 tgcattaacc gatgacaact atgcggtgat gaaagtggcg cgaattggct gcgaatgcgg
    38161 ggcaaatgtc tcgacttttt cctgcttcaa gctgtttggg ccggagggtg ttggcgcggt
    38221 ggtaggcgat gccgatgtta ttagtcggat tcgctccacg ctctattccg gcggcagcca
    38281 gattcagggc gctcaggcgc tggaagtctt gtgtgggctg gtgtttgcgc cagtgatgca
    38341 cgcggtgcag gcgggggtat ctgaacggtt gctggctttg cttaacggtg gtgcggtgcc
    38401 ggaagtgaaa agcgcggtga ttgctaatgc gcagtcgaag gtgttgattg tcgagtttca
    38461 tcagccgatt gccgccagag tgctggaaga ggcgcaaaag cgcggtgcct tgccttaccc
    38521 ggtgggtgca gagtcgaaat atgaaatccc gccgctcttt tatcgccttt ccggaacgtt
    38581 tcgccaggcg aatccacaat cagaacattg tgcgattcgc attaacccga atcgcaacgg
    38641 tgaagagacg gtgctgcgga ttttgcgtga gagtattgcc agtatttaat gctggatagc
    38701 accgtcaagc taaattccgt actgaacggt cccctcgccc ctttggggag agggttaggg
    38761 tgaggggaac gtacgggatg taaaattcaa cgcgccagca tcgccggatg cgatgctggc
    38821 gcatcttatc cggcctacgg ttaatgtctt ttattatcaa tcgttaaggt gatgcgattg
    38881 gcgcggcaga agagttcgga gacgtgcacc ggtttgtcat tctgatcaaa ggcgattttg
    38941 ctgatgcgaa acaacggttc gcccagttcg catttcagcc attttgcctg cgggcgggtg
    39001 gcggcaaaga tatcgatggt ctttttgtcg ctgaccactc gcgtgtcaaa acgctcctga
    39061 aataactgat aggtggagct tccttcgacg taaatctcat caaagtcagg ataacgcgac
    39121 agcgggatcc atgaactatc aataaacagc ggctctttat cgagatacat cacccggcag
    39181 agatggaaca cttcgctgtt tccggggatg ttcagctttt cgcaaaacgg cgcggcgctg
    39241 acccgttcct gttcgatcac tttctctttc gtcgccttgc cttgtgagac gccaaaatcg
    39301 gtaaaaccac tgacagtaag cagggcgttt tcaactttct ggctttgtac aaaggtgcct
    39361 ttgccctgcc agcggatcag tacgccgtct gccactaagt cgctgatagc tttgcgaata
    39421 gtaatgcggc tgacgttata ttgcgtacaa agctcgtttt cggtagggat ctgttgcccg
    39481 gcctggtaaa ccccctgcgc gatatcatcc agcagccgct ggcggacggt agcgtagagg
    39541 agttgatgag agtagcggtc cgtagctgac ataatcgacc ctgaaataaa gaatgaatta
    39601 cacggattat agggtcgttt tccggcaagt aacagcacgt tggcgcggag atgctcacgc
    39661 caacgttata cctaccaggc accgtgatac tgaatggttt tcgccgcgca cgccgttccc
    39721 tgcgccatcg cctgcggtaa tgtcatcccc gcagaccagc cgcaaaggaa tccggcaatg
    39781 aacgaatctc cggcacccat ggtgtcgata accgtcaccg gttcaggagc ctgacgccag
    39841 aactgcgcgc catcccaggc aatgctgccg ttttcaccca gcgtgacaat cactgttcct
    39901 gcgccacggg caacaatcgc tttcatcttc agacgcagcg cttcgtcttc ttgcggtgcg
    39961 gaggcaaagg caaaatcgag atgcggcacc agtgtctgcc agagcgggct gtcccacttg
    40021 tcggagaagt cgaaagcggt aagtttgccc gcagcgtgca gctgtgggaa tgcgtcttcc
    40081 gcatgtcccc agattgccgc gtgcacaatg tcatactgcg ccagccaggc gtaatcctct
    40141 tcactcaggg caaagtcggc catcacgcct tcggtgtagt cgccaaaaac gcgatcattg
    40201 tcgtgcagtt ccacctgagt ttgtgcggta acgccgtgtt tcgtatggac atggctgata
    40261 tcgacgccca tgcgggcgag atcctgcttc agctttgtgc cgtagtcatc gtcacccacc
    40321 aaggtaatgc atcccggctg tatgccgtag cgagtgcagt acaccgccac attgaccgca
    40381 ttaccgccag aaaacgcttt attcagttgc gggtagatat cgacgcagtt atcgccgatt
    40441 gtcgccaggg ttttcatctc tcatcctccg gcagcagcgc gcgaaagcgt tccagcgcct
    40501 ggcgggcata gagtctgggc tcgttcatat acatcgtcac cagctccacc gtacagtaac
    40561 cctcatagcc ccgctcaata atatcgcgca tcagttcccg cagcggcatt ttgccttctc
    40621 caggaatgta atgcgtgtcg ctggccccgt cgctgtcgac aatatgcaga tgacgataat
    40681 ttatcgccca gtttgtcgaa ataactcatc accggttccg cctggacata cggcgcgcaa
    40741 atgtcgacca tgctgaacag gcgcggcgaa ggcaccagcg ccagcgcatg aagcacatca
    40801 ttagcattac acacgacgtt cgattcatac ggcgttaacg gttcgaggat tagatccatg
    40861 ccgatgtttt ccgcgtactc acacagttcg cttaggttct ctgccagccg tccccagata
    40921 acattaggtg gcgtgagata gcctgcgtgg gccgcggaaa tcagcgtata acccgcgttc
    40981 atctcttttg ccatatccat cgccagcttg atcatgtcga ggctttcgcg acgcatatgt
    41041 tcatcgccca gcatcatgtt atacggatag ccgttggttt ctggcgtata gccgataatc
    41101 ggcatctgat acgtctgcgc cagcgccttg atttgtttga tgccgcccgc ttttaagtcc
    41161 ggcgcgaacg cgtgcgggcg accgccccaa atttcgatgc cgtcgtaacc cagctcgctt
    41221 gcatcacgaa atgcgtgttc aataggcagc cgctggtggc cgcaggtaaa catacctgtt
    41281 ttcatcgggt aactccttgc gtaaatccgg cccgttgccg ggccgggaga gggattaata
    41341 ttccaccaga ccaccgtaat agcggcgttc atccgggttg tgatctttgt aaatagacag
    41401 gtagtagcag agccactcca ttggcacgaa catcaggaac ggtgccagcc acgggtgcag
    41461 cccttgcgaa atttcggcgt aatcgatgac gatcacgttg tcagtacgct gtttaacaaa
    41521 gttaatggcg cgttcggtgg tgtggcgact ttcatcattg ccgagcagga acaggaacgg
    41581 aacgcccggt tcgacaatct ccagcgggcc atggcggaac tctccgctct caatcacgca
    41641 gccgtgcgtc caggtaaatt ccatcagcgt tacaatgcct tctttgtaac ccagcggacg
    41701 cagcggaccc gcagcaacgg tataaatcat cggccactgg ctggccagtt caccaagctg
    41761 gcggcctttt tcttcccagg tgcgtaccag atgaccgagc gcattcggca acggcttgag
    41821 atcgtttttg atcttgccga tttccgcgtt cggcgcgagg cgggtgatca tctccagcac
    41881 cacgctgtag cagagcagca ggtgaatttc ccagatacag tcggcctgat aatcaatgct
    41941 aaattccgcc gccgaggtaa tcgggctgtc cgcgcgtttg gtgaacgccg cagtgagtgc
    42001 gccgcaggcc cggcccagct ccagcgcttt gattacctct tccgttttac cgtagtcaga
    42061 aacgccaatc accgcgcaac gatcgtcgag gcgatacggg gtgttatcgc agaactccca
    42121 gccggaaatg gcgtagacct gaagatcgga aaagcgatcc gccagatgtt tcgccgtttg
    42181 cgccgcgttg agtggcgagc cgcaggcaac gaaataaata cgatcaatgt cgcgcttcac
    42241 catctcttcc acgatggcgt gcaccagcgg aacgtcatgg ctgagaactt tttccacttc
    42301 ctgaaccata ttttcggtga ccagaaagtc cacggtgctt ttatcaatat ccaacatttt
    42361 attttctcca gataacatta cgacaaagcg ttgagctggc ggctacgctt cgcccagaag
    42421 gcgtaagcag gcagaccagt agcaataacg atgacggcgc agataaggcc gggaatcggt
    42481 gcccagacaa aggttgaggc gaccagaatg aggcttgacg caatggcgag ggtggtcatc
    42541 agcccgaaag ccggagtacg ccacagcggt ttgtaatcgt cgcgtttacg acaccagatg
    42601 atggagccga aggtgagggt atttttgaaa cacatcacca gggtgaaata acccagcagg
    42661 ctggtgagat cggaaacgaa gatgaagaag atccccagcg ccccttgcag gatgatggag
    42721 acatccggcg tgttgtattt cggatgcaca tggccgaagc atttaaagaa caggttgtct
    42781 ttcgccatcg cgtattccag ccgcggctgg tacatcacgc agctggaaag cgaaccaaga
    42841 atgacgatca tcgccgtgat ggcaacaaag atcccagcgg tgctgccgag tgcggggatc
    42901 caggtcaggg cgtcggaaat cggcgtttca gaattggcga gtttgtcgaa gggcatcagg
    42961 ccggaaatca ccagcgccag cagggtgtag agcaccagaa ccagcagaca ggaaccaatc
    43021 agcgctcgtg gcatggtttt tccggggttt ttaatttcgc cggtcatata acagatagag
    43081 gccatgccgg tatacgacca actggtggca gagatccccg ccagcagcgc cataaagctg
    43141 cccgttgcgc caatcgcagt ggtggtaggg gcggcaaaat tctccgcttt aaaccagaag
    43201 atcccaaggc caatgacgat agtgaacggg ataattttgg cgatggtaat tagcgtctga
    43261 aacgctgcgc cgccttcaac ggagcgcagg tgtagcaaca taaaggcgat aattaatccg
    43321 gcggcgataa atttaccgag caacggatcg ataggcgtta aaaagccaag attgctgaca
    43381 atcgccagcg ccataatcga caatgacggc gcatcgttgg cccagaagct ggcccagccg
    43441 gagaggaaag ccagcggtcg gcttccggca tttttcagat aaacataatc tgcgccattt
    43501 tccggataag cggtggatag ttccgcatag acgcacattt gcgggatcac aattaacccg
    43561 ccaatgacaa acgcgagcac cgtaagccac ggcgtgcccg ctgcttttgc cacttcaccc
    43621 acagatacaa aaataccgga gccgacggtt gtcccgacgg cgattgcaag aacggcccaa
    43681 aatccgagct tgcgttggag ttcctggctt ccc
//
LOCUS       DAJTZL010000009        43529 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.009, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000009 DAJTZL010000000
VERSION     DAJTZL010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 43529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43529
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.009"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(53..129)
                     /locus_tag="OO544_000469"
     tRNA            complement(53..129)
                     /locus_tag="OO544_000469"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(93..95),aa:Arg,seq:acg)
     gene            complement(133..225)
                     /locus_tag="OO544_000470"
     tRNA            complement(133..225)
                     /locus_tag="OO544_000470"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(189..191),aa:Ser,seq:gct)
     gene            complement(541..726)
                     /gene="csrA"
                     /locus_tag="OO544_000471"
     CDS             complement(541..726)
                     /gene="csrA"
                     /locus_tag="OO544_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HCR6766163.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(961..3591)
                     /gene="alaS"
                     /locus_tag="OO544_000472"
     CDS             complement(961..3591)
                     /gene="alaS"
                     /locus_tag="OO544_000472"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HCR6766164.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(3719..4219)
                     /gene="recX"
                     /locus_tag="OO544_000473"
     CDS             complement(3719..4219)
                     /gene="recX"
                     /locus_tag="OO544_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HCR6766165.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALVRDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WLNFAD"
     gene            complement(4447..5508)
                     /gene="recA"
                     /locus_tag="OO544_000474"
     CDS             complement(4447..5508)
                     /gene="recA"
                     /locus_tag="OO544_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HCR6766166.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(5588..6085)
                     /gene="pncC"
                     /locus_tag="OO544_000475"
     CDS             complement(5588..6085)
                     /gene="pncC"
                     /locus_tag="OO544_000475"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HCR6766167.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(6230..7315)
                     /gene="mltB"
                     /locus_tag="OO544_000476"
     CDS             complement(6230..7315)
                     /gene="mltB"
                     /locus_tag="OO544_000476"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HCR6766168.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDRMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTRYSISQLATAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            7571..8134
                     /gene="srlA"
                     /locus_tag="OO544_000477"
                     /gene_synonym="gutA"
     CDS             7571..8134
                     /gene="srlA"
                     /locus_tag="OO544_000477"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HCR6766169.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            8131..9090
                     /gene="srlE"
                     /locus_tag="OO544_000478"
                     /gene_synonym="gutE"
     CDS             8131..9090
                     /gene="srlE"
                     /locus_tag="OO544_000478"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HCR6766170.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSSPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSHFLTGAPTVLIAWFVSGFIYQ"
     gene            9101..9472
                     /gene="srlB"
                     /locus_tag="OO544_000479"
                     /gene_synonym="gutB"
     CDS             9101..9472
                     /gene="srlB"
                     /locus_tag="OO544_000479"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HCR6766171.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFTLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            9476..10244
                     /gene="srlD"
                     /locus_tag="OO544_000480"
                     /pseudo
     CDS             9476..10244
                     /gene="srlD"
                     /locus_tag="OO544_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311588.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
     gene            10350..10709
                     /gene="gutM"
                     /locus_tag="OO544_000481"
     CDS             10350..10709
                     /gene="gutM"
                     /locus_tag="OO544_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HCR6766172.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDDQQRIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            10776..11549
                     /gene="srlR"
                     /locus_tag="OO544_000482"
                     /gene_synonym="gutR"
     CDS             10776..11549
                     /gene="srlR"
                     /locus_tag="OO544_000482"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HCR6766173.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFSFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            11542..12507
                     /gene="gutQ"
                     /locus_tag="OO544_000483"
     CDS             11542..12507
                     /gene="gutQ"
                     /locus_tag="OO544_000483"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HCR6766174.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTSAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(12504..14018)
                     /gene="norR"
                     /locus_tag="OO544_000484"
     CDS             complement(12504..14018)
                     /gene="norR"
                     /locus_tag="OO544_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HCR6766175.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMMPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VMAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRLGLSRVVLSAGAR
                     NLLQHYRFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGMKD"
     gene            14205..15644
                     /gene="norV"
                     /locus_tag="OO544_000485"
                     /pseudo
     CDS             14205..15644
                     /gene="norV"
                     /locus_tag="OO544_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
     gene            15641..16774
                     /gene="norW"
                     /locus_tag="OO544_000486"
     CDS             15641..16774
                     /gene="norW"
                     /locus_tag="OO544_000486"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HCR6766176.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDASIPLTLIAADSMDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAGQFNLHLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNDDIYALGDCAE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLPM"
     gene            complement(16902..19154)
                     /gene="hypF"
                     /locus_tag="OO544_000487"
     CDS             complement(16902..19154)
                     /gene="hypF"
                     /locus_tag="OO544_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HCR6766177.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLREDPETLLVQLHQHCPPLARIDSVEREPYIWSQLPTEFTIRQSAGGAMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRARKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPDLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVV
                     HDAHPGYVSSQWAREMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCHGVTHPVTMPLVDNQLDLAAFWQQWLNWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(19307..19834)
                     /gene="hydN"
                     /locus_tag="OO544_000488"
     CDS             complement(19307..19834)
                     /gene="hydN"
                     /locus_tag="OO544_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HCR6766178.1"
                     /translation="MNRFIIADASKCIGCCTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(19982..20992)
                     /gene="ascG"
                     /locus_tag="OO544_000489"
     CDS             complement(19982..20992)
                     /gene="ascG"
                     /locus_tag="OO544_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HCR6766179.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALAQHGIALNEKLIT
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAMKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            21252..22709
                     /gene="ascF"
                     /locus_tag="OO544_000490"
     CDS             21252..22709
                     /gene="ascF"
                     /locus_tag="OO544_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HCR6766180.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRVVIKDDQLIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPAQPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFIL
                     TLLLGFEDIPVEEAAAEARKHQSVQPTVAKEVSLN"
     gene            22718..24142
                     /gene="ascB"
                     /locus_tag="OO544_000491"
     CDS             22718..24142
                     /gene="ascB"
                     /locus_tag="OO544_000491"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HCR6766181.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGD
                     EITPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGTYPA
                     YSARVFREKGVTIDKAPGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(24256..24675)
                     /locus_tag="OO544_000492"
     CDS             complement(24256..24675)
                     /locus_tag="OO544_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6766182.1"
                     /translation="MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEE
                     DDTNPLIDFLASRIAEYENNNQEFAEFDKAVAAMPVGVALLRTLIDQHNLTYADLKNE
                     IGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL"
     gene            complement(24672..24983)
                     /locus_tag="OO544_000493"
     CDS             complement(24672..24983)
                     /locus_tag="OO544_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HCR6766183.1"
                     /translation="MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTA
                     FPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRFFVKHITNHAEYDKLTRYYRENK
                     E"
     gene            complement(25145..25918)
                     /locus_tag="OO544_000494"
     CDS             complement(25145..25918)
                     /locus_tag="OO544_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001026459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6766184.1"
                     /translation="MNIRTGMCAFLLSLVLPAQATSFTEYLPMSDSEYAQKRALKPLL
                     TMPYDAEQTWRFRKVGVAGVTLEKMPNDDSEWQLNGKDRAGKSWSVPVGMLANIAGKG
                     QLYRADLDRNGIQDLVIWLPSTGNGLAPYAHLILMTFTREGRPCVFEPRGFYTASKTG
                     VDDLLDLQGNGHTQLLDMQFDSGYWITSLYQVKDAKWQRVHGWFGKLSYPALTRFTYT
                     PNRKLVLKPIAGRDPQTEDLAQTQRCLIKGDVLDGVNQN"
     gene            complement(25943..26413)
                     /gene="hycI"
                     /locus_tag="OO544_000495"
     CDS             complement(25943..26413)
                     /gene="hycI"
                     /locus_tag="OO544_000495"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HCR6766185.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            complement(26406..26816)
                     /gene="hycH"
                     /locus_tag="OO544_000496"
     CDS             complement(26406..26816)
                     /gene="hycH"
                     /locus_tag="OO544_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HCR6766186.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQKEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            complement(26813..27580)
                     /gene="hycG"
                     /locus_tag="OO544_000497"
     CDS             complement(26813..27580)
                     /gene="hycG"
                     /locus_tag="OO544_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HCR6766187.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGEQDEQQAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDFLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            complement(27580..28122)
                     /gene="hycF"
                     /locus_tag="OO544_000498"
     CDS             complement(27580..28122)
                     /gene="hycF"
                     /locus_tag="OO544_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HCR6766188.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            complement(28132..29829)
                     /gene="hycE"
                     /locus_tag="OO544_000499"
     CDS             complement(28132..29829)
                     /gene="hycE"
                     /locus_tag="OO544_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HCR6766189.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDELYPLRKDSMDYRQRPAPTTD
                     AETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHRGMEK
                     LAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILLEVER
                     LHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIRRDLL
                     KDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEMARDFSNVGPMVRA
                     SGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLDNLPG
                     GPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPTLRYM
                     LRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNSPLK"
     gene            complement(29847..30770)
                     /gene="hycD"
                     /locus_tag="OO544_000500"
     CDS             complement(29847..30770)
                     /gene="hycD"
                     /locus_tag="OO544_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HCR6766190.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIFKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTAGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWTGFGFAFLAFVSLLAA"
     gene            complement(30773..32599)
                     /gene="hycC"
                     /locus_tag="OO544_000501"
     CDS             complement(30773..32599)
                     /gene="hycC"
                     /locus_tag="OO544_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HCR6766191.1"
                     /translation="MSAISLINSGVAWFVAAAVLAFLFSFQKALSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLVIACWLLWQRYGTLDLCLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGMLTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(32596..33207)
                     /gene="hycB"
                     /locus_tag="OO544_000502"
     CDS             complement(32596..33207)
                     /gene="hycB"
                     /locus_tag="OO544_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HCR6766192.1"
                     /translation="MNRFVIVDSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRIDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARISTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(33332..33793)
                     /gene="hycA"
                     /locus_tag="OO544_000503"
     CDS             complement(33332..33793)
                     /gene="hycA"
                     /locus_tag="OO544_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HCR6766193.1"
                     /translation="MTIWEISEKADYIAQRHCRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            34005..34355
                     /gene="hypA"
                     /locus_tag="OO544_000504"
     CDS             34005..34355
                     /gene="hypA"
                     /locus_tag="OO544_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HCR6766194.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            34359..35231
                     /gene="hypB"
                     /locus_tag="OO544_000505"
     CDS             34359..35231
                     /gene="hypB"
                     /locus_tag="OO544_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HCR6766195.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            35222..35494
                     /gene="hypC"
                     /locus_tag="OO544_000506"
     CDS             35222..35494
                     /gene="hypC"
                     /locus_tag="OO544_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HCR6766196.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            35494..36615
                     /gene="hypD"
                     /locus_tag="OO544_000507"
     CDS             35494..36615
                     /gene="hypD"
                     /locus_tag="OO544_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HCR6766197.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQENEA"
     gene            36612..37622
                     /gene="hypE"
                     /locus_tag="OO544_000508"
     CDS             36612..37622
                     /gene="hypE"
                     /locus_tag="OO544_000508"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HCR6766198.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARAAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISEVALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            37696..39773
                     /gene="flhA"
                     /locus_tag="OO544_000509"
                     /pseudo
     CDS             37696..39773
                     /gene="flhA"
                     /locus_tag="OO544_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
     gene            complement(39810..40163)
                     /locus_tag="OO544_000510"
     CDS             complement(39810..40163)
                     /locus_tag="OO544_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HCR6766199.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
     gene            complement(40240..40374)
                     /locus_tag="OO544_000511"
     CDS             complement(40240..40374)
                     /locus_tag="OO544_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6766200.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIASVE
                     "
     gene            40450..43011
                     /gene="mutS"
                     /locus_tag="OO544_000512"
     CDS             40450..43011
                     /gene="mutS"
                     /locus_tag="OO544_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HCR6766201.1"
                     /translation="MSAIENFDAHTPMMQQYLRLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            43117..43529
                     /locus_tag="OO544_000513"
                     /pseudo
     CDS             43117..43529
                     /locus_tag="OO544_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417214.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
BASE COUNT        10312 a        11945 c        11334 g         9938 t
ORIGIN      
        1 ttattttact gctgatattg ataccgctac taaagccata tcaagtaaga gatggtgcat
       61 ccgggaggat tcgaacctcc gaccgctcgg ttcgtagccg agtactctat ccagctgagc
      121 tacggatgca aatggcggtg aggcggggat tcgaaccccg gatgcagctt ttgaccgcat
      181 actcccttag caggggagcg ccttcagcct ctcggccacc tcaccacacg cctcttacga
      241 gtgcttcgaa gaacttgttt atcgctcatc gtcgctgcgt ggcgcacata ttactttctg
      301 agacttataa gtcaaacaat ttttcccaca cttttatcgt ttgcacactt cacgctcaat
      361 tagtctgtaa aaacgtcaaa aagagtgttt tatctacaga aaaacggagg tctggcagat
      421 agcagtaatg caaaaaaatg gagacttaag ttgaatgaac gggagtaaag cgaaaagact
      481 atagagtgaa ggagaaattt tgagggtgcg tctcaccgat aaagatgaga cgcggaaaga
      541 ttagtaactg gactgctggg atttttcagc ctggatacgc tggtagatct cttcacggtg
      601 aacagaaact tccttcgggg catttacgcc aatacgtacc tggttgccct ttacccctaa
      661 aactgtcacg gtgacctcat ccccaatcat gagggtctca ccaactcgac gagtcagaat
      721 cagcattctt tgctccttga aagattaaaa gagtcgggtc tctctgtatc ccggcattat
      781 ccatcatata acgccaaaaa gtaagcaatg acaaacacat tacatgtaag cagtcatggc
      841 attacattct gttaaaccta agtttagccg atatacacaa cttcaacctg actttatcgt
      901 tgtcgatagc gttaatgcga atgccgtgaa gcgagtccac ggcattgcct gacgcttata
      961 ttattgcaat ttcgcgctga cccagccttt cacactggct aacgctgcag gtaaggccgc
     1021 agcatccgta ccaccggctt gcgccatgtc aggacgtcca ccacccttgc cgcccacctg
     1081 ctgagcgacc ataccaatca gttcccctgc tttcacacga tctgtgacgt ccttagatac
     1141 gcctgcaatc agagaaacct taccttcggc taccgttgcc agcacgataa ttgtcgaccc
     1201 cagctgattt tttaaatcgt caaccatggt acgcaacatt ttcggctcaa caccgctaag
     1261 ctcgctaacc aacagcttaa caccattaac atcaattgcc ttactggaaa gatttgcgct
     1321 ctcctgtgcg gcagcttgtt ctttaagctg ttgtagttct ttttctaact gacgcgtacg
     1381 ttccagtact gagcgcactt tatccgccag attattgcta tcgcctttca gcagatgcgc
     1441 gacttcgctt aagcgatcgc tgtctgcatg aacggtggtg atagcgcctt ctccggttac
     1501 cgcttcgata cgacgaacgc ctgcagcagt acccgattca gagatgatgc ggaacagacc
     1561 aatatcacca gtgcggctgg cgtgagtacc gccacacagc tcggtggaga aatcgcccat
     1621 gctcagcacg cgtacacgct catcatactt ctcgccgaac agcgccatcg cacctttcgc
     1681 tttcgccgct tcgagatcca tgatgttggt ttcgatcggc aaattgcgac gaatctgtgc
     1741 gttcaccagg tcttcgaccg cacgaatctc ttccggtttc atcgcttcgt tgtgtgagaa
     1801 gtcgaagcgc agcaccttgt cgttaaccag tgaacctttc tgcgatacat gagtccccag
     1861 aacctggcgc agcgcagcgt gcatcaggtg cgttgcggag tgattcagac gaatacgggc
     1921 gcgacgagcc tcatcaacat cagcctgcac agcgtcgccc actttcagag aacccgcagc
     1981 aagtttaccg atgtgaccaa tcgcctggcc gtatttctgc gtatcttcca ccgcgaagga
     2041 gaagttagcg cctttcagtt cgcctttatc accaacctga ccgccggatt ccgcatagaa
     2101 tggcgtttga tccagcacga ccacagcttc ctggcctgca ttgatggcat caaccgcttt
     2161 accatcaaca aacagcgcgg tcactttgcc gttcagttcc agatggtcat agcctttaaa
     2221 ttcagatgca ctatcaacac ggatcattgc gttgtaatcg gcaccaaagc cgctggcttc
     2281 gcgcgcgcga cgacgctgct cttccattgc tgcttcaaaa ccagcttcgt caactttgat
     2341 gttgcgctca cgacaaacat cagccgtcag gtcaaccggg aagccatagg tgtcgtacag
     2401 acggaaagcc gtttcaccat ccagcgtatc accagaaagt tttgccagct cttcatccag
     2461 caacgccaga ccgcgctcca gagtacgggc aaactgctct tcttcagtct tcagcacctg
     2521 ctcaacctgc gcctgctggc gtttcaggtc ttcacccgca gagcccataa cgtcgatcag
     2581 cggaccaacc agtttgtaga agaaggtttc tttcgcgccg agcatattgc cgtgacgcac
     2641 tgcgcgacga atgatacgac gcagtacata accacggttt tcattggacg gcattacgcc
     2701 atccgcgatc aggaacgcac aagaacgaat gtggtcagcg attacgcgca gcgatttatt
     2761 gctcagatcg gtcgcgccag tgactttcgc taccgcctgg atcaacgtgc ggaacaggtc
     2821 gatgtcatag ttagagttaa cgtgttgcag caccgcagca atacgctcca gacccatacc
     2881 ggtatctaca gacggcttcg gcagcggttc catcgtgcca tctgcctggc ggttgaactg
     2941 catgaagacg atgttccaga tctcaatgta gcggtcgccg tcttcttccg gacttcccgg
     3001 agggccaccc caaatgtggt cgccgtgatc gtagaagatt tcggtgcacg ggccgcacgg
     3061 accagtgtca cccatctgcc agaagttgtc agatgcgtat ggcgcacctt tgttatcgcc
     3121 gatgcgaata atacgttcgc gcgggatccc tacttctttt tcccagattt cgtaggcttc
     3181 gtcgtcgctt tcatagacgg taacccacag acgctctttc ggcagggcaa accatttttc
     3241 gctggtcagc agttcccatg caaactgaat ggcatcgtgt ttgaaatagt cgccgaagct
     3301 gaagttgccc agcatttcga agaaggtatg gtgacgcgcg gtgtaaccga cattttccag
     3361 gtcgttgtgt ttaccacccg cacgcacgca gcgttgggaa gtggtagcgc gggaataatt
     3421 acgcttgtcg agtccaagga acacatcctt gaactggttc atcccggcgt tggtaaacaa
     3481 caaagttggg tcgttatggg gtaccaggga gctgctggca actacctgat gtcccttact
     3541 atggaaaaag tcgagaaacg cctgacggat ctcagcggtg ctcttgctca taattatcct
     3601 gaaatcaagc taacgaaata tcgccaccag ctccagcgtg tcttaaccgc cgggctggta
     3661 actgaaaagt gggaataaga taagttttct tgagtgggaa gtaaaatacc gtatgcgttc
     3721 agtcggcaaa atttagccaa atatcctgga tatcttccat cagatagcca cgatagagca
     3781 gaaaacgctg gatcttaact ttttctgaaa agacagttgg caaaggttca ccatattttc
     3841 gcgtcgcctg atcgcgcacc agtgcgcacc agtcgatgtc acattcacgc atcgcttttt
     3901 ctgtcgcttc gcgggaaata cctttctgat tcagttcctg acgaatacgt gcaggtccat
     3961 aacctttgcg gctacggctg gcgataaagc gcgcaacaaa tcggctgtca tcgagatagc
     4021 catgttcatg gcaccaggca ataacgcgct cgtaatcttc tgccgtagca tcaatctctt
     4081 ctgggccatt tttgcccata atcggtgccg cgagtttacg tcgcagttct tgctcactgt
     4141 gatcgcgcac cgccagaatg cgtaccgcac gatccaacag gcgagcatat gccgggcgac
     4201 gggatgttga ttctgtcatg gcatatcctt acaacttaaa aaagcaaaag ggccgcagat
     4261 gcgacccagg ttttgctgat aaacagattt aatagtcaat gagtgtgcag ccaattcgtt
     4321 agaggatcga ggtgagagta atttttcacg ccagtgttgg cctgttcccc tcaccctaac
     4381 cctctcccca aaggggcgag gggaccgtcc gtgcacacat tatctattat catcaataac
     4441 gaacgattaa aaatcttcgt tagtttctgc tacgccttcg ctgtcatcta cagagaaatc
     4501 cggcgttgag ttcgggttgc tcagcagcaa ctcacgtact ttcttctcga tctctttcgc
     4561 ggtttccggg ttatctttca gccaggcagt tgcattcgct ttaccctgac cgatcttctc
     4621 acctttgtag ctgtaccacg cgcctgcttt ctcgatcagc ttctctttta cgcccaggtc
     4681 aaccagttcg ccgtagaagt tgataccttc gccgtagagg atctgaaatt cagcctgttt
     4741 aaacggtgca gcgattttgt tcttcaccac tttcacgcgg gtttcgctac ccaccacgtt
     4801 ttcgccctct ttcaccgcgc cgatacgacg gatgtcgaga cgaacagagg cgtagaattt
     4861 cagcgcgtta ccaccggtag tggtttccgg gttaccgaac atcacaccaa ttttcatacg
     4921 gatctggttg atgaagatca gcagcgtgtt ggactgcttc aggttacccg ccagcttacg
     4981 catcgcctgg ctcatcatac gtgccgcaag gcccatgtga gagtcgccga tttcgccttc
     5041 gatttccgct ttcggcgtca gtgccgccac ggagtcaacg acgataacgt ctactgcgcc
     5101 agaacgcgcc agggcgtcac agatttccag tgcctgctcg ccggtgtccg gctgggagca
     5161 cagcaggtta tcgatatcga cgcccagttt acgtgcgtag attgggtcca gcgcgtgttc
     5221 agcatcgata aacgcacagg ttttaccttc acgctgcgct gcggcgatca cctgcagcgt
     5281 cagcgtggtt ttaccggaag attccggtcc gtagatttcg acgatacggc ccatcggcag
     5341 accacctgcc ccaagcgcga tatccagtga aagcgaaccg gtagagatgg tttccacatc
     5401 catggaacgg tcttcaccca ggcgcatgat ggagccttta ccaaattgtt tctcaatctg
     5461 gcccagtgct gccgccaacg ctttctgttt gttttcgtcg atagccattt ttactcctgt
     5521 catgccgggt aataccggat agtcaatatg ttctgttgaa gcaattatac tgtatactca
     5581 tacagtatca agtgttttgt agaaattgtt gccacaaggt ctgcaatgca tacgcagtag
     5641 cctgacgacg caccgcatca cggtcgccgc tgaagcattc ccgccgggta atgccttcac
     5701 cgcgggcagt ggcaaaagca aaccagacgg tgccgacagg cttctcttca ctgccgccat
     5761 ccggcccggc gataccacta atagacacgg cataatcagc acgagccgct ttcagtgcac
     5821 ctatcgccat ttccaccacg acgggttcac tcaccgcgcc atgctgcgcc agcgtctctt
     5881 cgcgtacgcc gatcatctgc gctttggctt cgttactgta ggtgacaaat ccgcgttcaa
     5941 accaggcgga gctaccggca atatcggtaa tcactttcgc tacccaacca ccggtacaag
     6001 actcggcagt tgttacggtt gcgccacggg ctttcagcgc ctgcccaacc tgttcgctta
     6061 actgcatcag ttcactgtca gtcatcacaa cctcagtcag tcaaaaattt atgccggaca
     6121 agatagcact ttcgtcagga agatggtgtt ggcgttgcaa ttttacgagg aggattcaga
     6181 aaaaagctga ttagccagag ggaagctcac gcccccctct tgcaaatagt tactgcacgc
     6241 gcgccagcgc cacggcttgt cctaactgcc agaccgccat tgcgtaatgg gtgctgtggt
     6301 tataacgggt aatggtgtag aagttcggca gaccgtacca gtactggtaa ccggtaccga
     6361 catccagacg cagcaggctg gcttgttgat ggttgcccag cggctgctgt ggcgttaaac
     6421 ctgccgtggc aagctgcgaa atgctgtacc tggttttgaa gccatttggc aagcctggag
     6481 cctgaccgtt tgccattacc gcgacctgat cgcctttcac ccagccgtgc gctttgaaat
     6541 agttcgccac actaccgatc gcatcaaccg gatcccacag gttgatatgc ccgtcgccgc
     6601 tgaaatctac cgcatattgt ttgtaagacg acggcataaa ctgtccgtag cccatcgccc
     6661 cggcaaagga acctttcaga ttgagcggat cgtcctgctc gtcgcgcgcc atcaacagga
     6721 aggtttccag ctcgccagag aaatactccg cgcggcgtgg gtagttaaat gacagcgttg
     6781 ccagcgcatc gaggatgcga gttttcccca tcacgcgccc ccagcgggtt tcaacgccga
     6841 taatcccgac gataatttcc ggcggtacgc catacacctg ccacgcgcga ttcaacgcat
     6901 cttcatactg attccagaaa accacaccgt tctgcacgtt gtccggcgta ataaattttt
     6961 tgcgataacg cagccatgcg ccgttcggac ctgatggcgg tttcaccgat gtggttggtg
     7021 cctggttatc catcagccgc agtaccgaat ccagacgctt cgcctgggag agaatttcct
     7081 gcaactgctg acgatcgaaa ccgtgtttgt tcaccattct gtcgatgaac tgctgggcat
     7141 tcgggttatt agcgaaatcg ccgcccatct gcatcacatt gtgctgaggc tcaagcagga
     7201 agccgccaga cggcgttccg gtggtcgttt cagtctcagt aggttttggc ttgctgctac
     7261 aggcggcaag caacacaaaa aggggaagca atgttacata acgacgcttg aacatgaggg
     7321 gtccatttaa cagattcaac caggggcaag tatggtaaag catcacgccc cgcacaagga
     7381 agcggtagtc actgcccgat acggacttta cataactcaa ctcattcccc tcgctatcct
     7441 tttattcaaa ctttcaaatt aaaatattta tctttcattt tgcgatcaaa ataacacttt
     7501 caaatctttc aatctgatta gattaggttg ccgtttggta ataaaacaat aaatcctgaa
     7561 ggagagaaca atgatagaaa ccattactca tggtgcagag tggtttatcg ggctgttcca
     7621 aaagggcgga gaggtgttta ccgggatggt gaccggcatt cttccgctgt tgattagcct
     7681 gctggttatc atgaacgcac tgattaattt tatcggtcag catcgtattg aacgttttgc
     7741 tcaacgttgc gccggtaacc ctgtttcccg ttacctgctg ctaccgtgca ttggcacgtt
     7801 tgtcttttgc aatccgatga ccttaagcct gggtcgcttt atgccggaaa agtacaaacc
     7861 cagctactac gcggcagcct cttatagctg ccactcaatg aatggcctct ttccccatat
     7921 caaccctggc gaactgtttg tttatcttgg cattgccagc ggtctgacaa cgctgaacct
     7981 gccacttggc ccactggcgg tgagctatct gctggttggt ctggtcacca acttctttcg
     8041 cggctgggtg accgatctga ccaccgccat ttttgagaaa aagatgggca ttcaacttga
     8101 acaaaaagtt cacctggcag gagcaacatc atgacgcgta ttcggatcga aaaaggaacg
     8161 ggtggctggg gcggcccgct tgagctggaa gccacgccgg gcaaaaaaat cgtctatatc
     8221 accgccggta cccggcctgc gattgttgac aaactggcac aacttactgg ctggcaggct
     8281 attgacggat ttaaagaagg tgaacccgcg gaggcggaaa ttggtgtcgc ggtaatcgac
     8341 tgtggcggca cattacgctg cggcatctat ccgaaacgac gtattcccac cattaatatc
     8401 cactcgacgg gcaagtccag tccgctggcg cagtacattg tggaagatat ttatgtctct
     8461 ggcgtaaaag aagaaaacat cactgtagtg ggtgatgcga caccacaacc ctcttccgtg
     8521 ggccgtgact atgacaccag caagaaaatc accgaacaaa gcgatggttt actggcgaag
     8581 gtgggaatgg gtatgggttc tgccgttgcc gtgttgtttc aatctggtcg tgacaccatc
     8641 gacactgtat taaaaaccat tctgccgttt atggcgttcg tctcggcgct cattggcatc
     8701 attatggctt ctggccttgg tgactggatt gcccacggtc ttgctccgct ggcgagccat
     8761 ccactaggtc tggtcatgct ggcgctcatc tgctccttcc cgctgctttc acctttcctc
     8821 ggcccaggcg cagttatcgc acaggttatc ggcgtattga ttggcgtgca gattggtctc
     8881 ggcaatattc cgccgcatct ggctttaccg gcactgtttg ccatcaacgc gcaggcggcc
     8941 tgcgacttca tcccggtcgg tttgtcgctg gcggaagccc gtcaggacac ggttcgcgtc
     9001 ggtgtccctt ctgtactggt gagccacttt ttaaccggcg caccgactgt actgatcgcc
     9061 tggtttgtct ccggttttat ctatcaatag aggctgaaac atgaccgtta tttatcagac
     9121 caccatcacc cgtatcggcg cgagtgccac tgacgccctc agcgaccaga tgctcatcac
     9181 ctttcgtgaa ggcgcgccag cggacctcga agagtattgc ttcattcatt gccacggcga
     9241 gttgaaaggt gcactccatc ccggtttgca atttacgctc gggcagcatc gctatccggt
     9301 gaccgctgtt ggcagcgtgg cggaagacaa ccttcgcgaa ctgggtcatg tcaccctgcg
     9361 cttcgatggt ttaaacgaag cggaatttcc gggcactgtc catgtggcag gccctgtacc
     9421 cgacgatatc gcgccgggat cggttttgaa gtttgaatct gttaaggagt aaaaaatgaa
     9481 tcaggttgcc gttgtcatcg gtggtgggca aaccttaggc gcgttcctgt gccacggtct
     9541 ggctgccgag gggtatcgcg tcgcggttgt cgatattcag agcgacaaag ccgcaaatgt
     9601 ggcacaagaa attaacgccg aatatggtga aggaacagcg tatagttttg gtgctgacgc
     9661 caccagcgag caaagcgtcc tggcgctctc tcgtggggta gatgaaatct ttgggcgcgt
     9721 ggatttgctg gtctacagcg ccggaatagc aaaagcagcc tttatcagcg acttccagct
     9781 cggcgatttt gaccgtgaat ctggtgggtt atttcctgtg tgcccgtgaa ttttctcgtt
     9841 tgatgatccg cgacgggatt cagggtcgca ttattcagat caactcgaaa tccggcaaag
     9901 tgggcagcaa acacaactct ggctacagcg cagcgaaatt tggtggcgtc gggctgactc
     9961 aatcactggc gctggatctg gcggagtacg gcattacggt gcattcactg atgctcggta
    10021 acctgctgaa atcgcctatg ttccagtcac tgttacccca atacgcgacc aagctgggta
    10081 tcaaaccgga tcaagtcgag cagtattaca tcgacaaagt accgctcaaa cgcggctgcg
    10141 attatcagga tgtgctgaat atgctgctgc tctatgccag tcctaaggcg tcgtactgca
    10201 ccggacagtc gatcaatgtc accggcggtc aggtgatgtt gtaatcaaca gcggagatcc
    10261 gttaaggatc tccgtgagac taatagaatg cctgatgcgc tacgctcatc aggcatacag
    10321 gacttccgcc actacattaa ggaaaagtta tggtatccgc actcatcacc gtcgccgtta
    10381 tcgcctggtg tgcgcaactg gccttaggcg gctggcaaat ttctcgtttt aaccgtgcct
    10441 tcgacacact atgccagcaa gggcgggttg gcgtgggccg ttccagcggg cgctttaaac
    10501 cacgggtcgt ggtcgccatc gccctggacg atcagcagcg catcgtcgac accttgttta
    10561 tgaaaggact gaccgtcttc gcacgaccgc aaaaaattcc cgcaattacc ggtatgcatg
    10621 cgggtgattt acagcccgat gtgatctttc cccatgatcc actatcacag aatgctctat
    10681 cattggcgct taaactgaaa cgtggataat ttcgttgtga atgttacttg cttgcgaagt
    10741 tatcattttg aaacctaaat caggtaatca cgcccatgaa acctcgtcag cgtcaggccg
    10801 ccattctgga gtatctgcaa aagcagggta aatgctcggt tgaagaattg gcgcaatact
    10861 ttgacaccac aggcacaacc attcgcaaag atctggtcat tctggaacat gcaggaaccg
    10921 tcattcgtac ttatggcgga gtggtgttga ataaagagga atccgatccg cctatcgatc
    10981 ataaaacact catcaacacc cacaagaaag agctgatagc agaagctgcc gttagtttta
    11041 ttcatgatgg cgattcgatc attcttgatg ctggcagtac cgttttgcag atggttcccc
    11101 tgctctcgcg ctttaataac atcacggtga tgaccaacag tctgcatatc gtcaatgcgc
    11161 tatctgaact ggataacgaa caaactatcc tgatgccagg cggaacgttt cgtaaaaaat
    11221 cggcctcatt tcacggacag ctggcagaga atgccttcga acatttcagt ttcgataaat
    11281 tgtttatggg caccgacggc atcgatctca atgcgggcgt aaccaccttt aacgaggttt
    11341 ataccgtcag taaggcaatg tgcaatgccg cgcgcgaagt gattttgatg gcggactcat
    11401 caaagtttgg ccgtaaaagc cccaacgtag tttgcagtct tgaaagcgtc gataagctga
    11461 ttaccgacgc aggtatcgat ccggcgtttc gtcaggcgct ggaagagaaa gggatcgatg
    11521 tgatcataac cggagagagc aatgagtgaa gcactactga acgcgggacg tcagacgtta
    11581 atgctggagt tgcaggaagc aagccgttta ccggaacgtc tgggcgatga ttttgttcgc
    11641 gccgccaata tcatcctgca ctgcgaaggc aaagtggtgg tttcgggaat tggcaaatcg
    11701 ggccacattg gtaagaaaat cgccgcaaca cttgccagta ccggcacatc ggcttttttt
    11761 gtccatccgg cagaagcact gcacggcgat ctggggatga ttgaaagccg cgatgtgatg
    11821 ctgtttatct cttactccgg tggcgcgaag gaactggatc tgattattcc gcgtctggaa
    11881 gataaatcta tcgcgctgct ggcgatgacc ggcaaaccga cgtcaccgct gggcctggcg
    11941 gcaaaagcgg tgctggatat ctccgtagaa cgcgaagcct gcccgatgca ccttgcgccg
    12001 acctccagca ccgtcaatac cctgatgatg ggtgacgcgc tggcgatggc ggtcatgcag
    12061 gcgcgcggat ttaatgaaga agattttgcc cgctcccacc cagccggggc actgggcgct
    12121 cgcttgctga ataaagtgca tcatctgatg cgccgtgacg atgccatccc acaggtggcg
    12181 ttaaccgcca gcgtgatgga tgcgatgctg gaactcagcc gcaccggtct ggggctggtg
    12241 gcggtatgtg acgctcaaca acaggtacaa ggcgtcttta ccgacggcga tttacgtcgc
    12301 tggctggtag gcggcggcgc actcaccacg ccagttaatg aagcgatgac gacaggcggc
    12361 accacgttac aggcgcaaag tcgcgccatc gacgccaaag agatcctgat gaagcgcaaa
    12421 atcactgccg caccggtggt ggatgaaaac ggcaaactca ccggcgcaat taacctgcag
    12481 gatttctatc aggccgggat tatttaatcc ttcattccca gacgtttcgc cagccgatgc
    12541 aggttggcga cgtcggtttc cagcatccgc gcgcaggcag cccagttatg atgattttgt
    12601 gccagtgcct ggcgaatagt ttcacgctgg aacgcttctg tcgcttcacg caggttttgc
    12661 ttaacaacgg gcaccgccgc cgcttctggc ggcggcaacg tcacctcagg aaaagcaaaa
    12721 tgttgcgcct caagaatcac ttcatcgccg ttgcgggtgg ctctcgccag cactaccgcc
    12781 cgatgaatag catgttccag ttcgcgcacg ttccccggaa aacgatagtg ttgcagtaaa
    12841 tttcgcgctc cggcacttaa taccacgcgg gagagcccca gccgcaaacg acactgctcg
    12901 cagaaatacc ccgccagcag aatgacatca tcgccccgct cacgcagcgg cggcaccgaa
    12961 agtggaaaca cgctcaggcg atgaaacaag tcagcgcgaa atcgccctgc catcacctct
    13021 tcgcgtaagt cgcggttagt cgccgccagc acgcgcacat cgacccgcaa actacggtca
    13081 tcgccaacgc gctgaatatc gccatactgc aacaccctca gcagcttggc ctgcaatgcc
    13141 aacgacaact cgccgatctc atccagaaac agagtgccgt tatccgccat ttcaaacttc
    13201 ccgctgcggt tactgatagc gccagtaaac gctcctttca catgcccgaa caactcactt
    13261 tccgccacac tttccggcag tgcagcacag ttgagataga ccagcggatt caccgcccgt
    13321 ggcgaggctt catgaatcgc tttcgccacc agctccttac cggttcccgt ctcaccgctg
    13381 atcaggacgt tgagatcgga cgccgccaca atctcaatct cttttttcag ttgcgtcatg
    13441 ccaggggaca agccaatcat ctgcgtctgt ttcaccgctt caaacggcgt ggcatcgcct
    13501 ggcatcatat tctggctttc cagttgttca atcagcaacg cattgcttaa cgctcccgcc
    13561 gccagcgcag caatcagccg tagctcttcg tcgctgaaaa catcgaactg atcgggctgc
    13621 atcccgtcga gcgtcagtgc gccgatcagg ttttgcccgg caaacaatgg cagaccaacg
    13681 caggcgtgaa ccttcagact ctcctgccca ggaatcaaac cgtcataggg atcgggcaat
    13741 tcgctgtctg cgggaaagcg caccacatcc ccggcgcggg caatcgcttc cagccgtgga
    13801 tgcccttcca gcgcaaagcg tctaccgagt acatcctttg ccagaccgtc gatggcaagc
    13861 ggaataaact gccgcgaatc gtaacgtagc aacgcagacg catcgcactc cagcacctga
    13921 cgtagcgtgg tgatcaggcg ctgaaaacga tcctgatgac caatcccacg ctgcaattcg
    13981 atggcgatat tcgccagcac atcaacggaa aaactcatct ttgcctcact gtcaatttga
    14041 ctatagatat tgtcatatcg accatttgat tgatagtcat tttgactact cattaatgag
    14101 cataatttta tttatagagt aaaaacaatc agataaaaaa ctggcacgta atctgcaatt
    14161 agcaagacat ctttttagaa cacgctgaat aaattgaggt tgctatgtct attgtggtga
    14221 aaaataacat tcattaggtt ggtcaacgtg actgggaagt gcgtgatttt cacggtacgg
    14281 aatataaaac gctacgcggc agcagttaca acagctacct catccgcgaa gaaaaaaacg
    14341 tgctgatcga caccgtcgac cataaattca gccgcgaatt tgtgcagaac ctgcgtaatg
    14401 aaatcgatct ggcggatatc gattacatcg tgattaacca tgcagaagag gaccacgctg
    14461 gggcgctgac cgaactgatg gcgcaaattc ccgatacgcc gatttactgt actgccaacg
    14521 ctatcgactc gataaatggt catcatcatc atccggagtg gaattttaat gtggtgaaaa
    14581 ctggtgacac gctggatatc ggcaacggca aacagctcat ttttgtcgaa acgccaatgc
    14641 tgcactggcc ggacagcatg atgacttacc tgacaggcga cgcggtgctg ttcagtaacg
    14701 atgctttcgg tcagcactac tgcgacgagc atctgttcaa cgatgaagtg gatcagacgg
    14761 agcttttcga gcagtgccag cgttactacg ccaatatcct gacgccgttc agccgcctgg
    14821 taacaccgaa aattaccgag atcctgggct ttaacttacc agtcgatatg atagcgacct
    14881 ctcacggcgt ggtatggcgc gataacccga cgcaaattgt cgagctgtac ctgaaatggg
    14941 cggctgatta tcaggaagac agaatcacca ttttctacga caccatgtcg aataacaccc
    15001 gcatgatggc tgacgctatc gcccagggga ttgcggaaac cgacccacgc gtggcggtga
    15061 aaattttcaa cgtcgcccga agcgataaaa acgaaatcct gaccaatgtc ttccgctcaa
    15121 aaggcgtgct ggtcggcact tcgacgatga ataacgtgat gatgccgaaa atcgccggtc
    15181 tggtggagga gatgaccgga ttacgcttcc gtaacaaacg cgccagtgct ttcggctctc
    15241 acggctggag cggcggtgcg gtggatcgtc tttccacgcg cctgcaggat gcgggtttcg
    15301 aaatgtcgct tagcctgaaa gccaaatggc gaccagacca ggacgctctg gagttatgcc
    15361 gtgaacatgg tcgcgaaatc gcccgtcagt gggcgctcgc gccgctgccg cagagcacgg
    15421 tgaatacggt ggttaaagaa gaaacctctg ccaccacgaa gtccgacctc ggcccacgga
    15481 tgcagtgcag cgtctgccag tggatttacg atccggcaaa aggcgagcca atgcaggacg
    15541 ttgcgccagg aacgccgtgg agtgaagttc cggataactt cctctgcccg gaatgctctc
    15601 tcggcaaaga cgtctttgac gaactggcat cggaggcaaa atgagcaacg gcattgtgat
    15661 cattggttcg ggcttcgccg cccgccaact ggtgaaaaat attcgcaaac aggacgccag
    15721 tattccatta accctgatcg ccgccgacag catggatgag tacaacaaac cggacctcag
    15781 ccatgttatc agccaggggc aacgtgccga cgaccttacc cgccagacgg cgggtgaatt
    15841 tgccgggcag tttaatctgc acctgtttcc gcacacctgg gtgacggata tcgatgccga
    15901 agcccatgtg gtgaaaagcc agaataatca gtggcaatac gacaagctgg tgctggcaac
    15961 tggtgccagt gcctttgtcc cgccagtgcc tgggcgtgag ttaatgctga cgttaaatag
    16021 tcagcaagag tatcgcgcct gtgaaacgca actgcgggat gcccgacgcg tgttgattgt
    16081 cggcggtggt ttgattggta gcgaactggc gatggatttt tgtcgtgcag gcaaagcggt
    16141 cacgctaatc gacaacgctg ccagtattct ggcgtcatta atgccaccag aagtaagcag
    16201 ccgcttgcag catcggttga cggagatggg cgttcatctg ctgttgaaat ctcagttgca
    16261 ggggctggaa aaaacggatt ctggcattct ggcaacgctg gaccaccagc gtagtatcga
    16321 agtggatgcg gtaattgccg ccaccggact gcgcccggaa acagccctgg cacgacgcgc
    16381 cgggctgacg attaatcgcg gcgtttgcgt cgatagttat ctgcaaacca gtaatgacga
    16441 tatttatgcg ctgggcgatt gcgcggaaat caacggtcag gtactgccat tcctgcagcc
    16501 gattcaactt agcgcgatgg tgctggcaaa aaatcttctc ggcaataaca cgccgctgaa
    16561 actaccggcg atgctggtga aaatcaaaac gccggaatta ccgctgcatc tggcaggcga
    16621 aacccagcgt caggatttac gctggcaaat taataccgaa cgccagggaa tggtggctcg
    16681 cggtgttgac gatgctgacc agcttcgcgc ctttgtggtc agtgaggatc ggatgaaaga
    16741 ggcatttgga ttgttgaaaa cattgccgat gtaggtgggc tactgtgcct aaaatgtcgg
    16801 atgcgacgct ggcgcgtctt atccgaccta cggggacgca tgtgtaggcc agataaggcg
    16861 tttacgccgc atccggcaat ggtgtccaaa tgcaacacgt tttatccgtt ctggacttca
    16921 cccgctaacc aacgcgccgc agcaataacc ccctgcccca gagacaaacc gccatcaccc
    16981 gccggtaaac tctgtggaaa gagcaatgtg aaatcagcga gataatgcgc cagacgtgca
    17041 cgcagcaaac ggttatgaat aaccccgccg ctaaatacca gcgtagtgat accacgcatc
    17101 gtggcctgct cacgcatcaa cgcggcaaaa ccctgcgcca gcgcatcatg aaacgcccac
    17161 gcgcgttgat taaccggtgc ctgccagttc agccactgct gccagaaagc ggcgagatcc
    17221 agttgattgt ccaccagcgg catcgtcacc ggatgcgtca ctccgtggca tgaggctgcg
    17281 agcgcctcca gagcacaagc cgcttcacct tcataactta acgtggctgg cgcacagccc
    17341 agtgccgccg ccactgcatc gaacaaccgc ccacacgatg acgccagcgg cgcgttaatt
    17401 ccacgctcaa tggcccgcgc cagcacgctc cagttttgct gttgcacact tgctgtttca
    17461 gggtaattct gccactccgg cacaaagcgc aggcactgcg ccagcaggtt tcgccacggc
    17521 tgcttcgctg ccaaatcgcc acccggaagc gccactgcag gcaagccgcc caggtgctca
    17581 cattcgcgat agttcacccg caggcactcg ccgccccaca aagcgccgtt ctcccccata
    17641 ccgataccgt cgagcgtcaa agcaatgaca tcaccgccat ccaacggcca ctgatgctct
    17701 gccagacacg ccgctgcatg ggcatgatga tgcagtaccg tttgcgtcgg cagattcatt
    17761 tcacgcgccc actggctgga gacatagccc ggatgcgcgt catgcacaac gtattgcggg
    17821 gtaaaatcgt agatgttttg catcaggcgt aacgcttcgc gccactgcat ctggatgcca
    17881 tcgtcactta aatcgcccag atgctgactc aacaccgctt gttcaccgcg caccaggcag
    17941 aaggtatttt tcagatccgc gccgagacac agcacaggcg gaacattttt aaagcccgga
    18001 ggcaaagcca gcgcatccgg cacatacccc cgcgaacggc gcagcatttc gccgctttcg
    18061 cgcaccaccg aatcatccat ccgctgcacg atgtcgcggt tatgtatcaa gaatccgtcg
    18121 gcaatgccct gcaaatccgc cagcgcctgt tcgttgctga tagctggtgg tttaccgctc
    18181 aggttgccgg aggtcatcac cagcgggcat tgcagttcct gtaacagcaa atgctggagc
    18241 gggttcgcag gcaacattac cccgacttcg ttaaggtcag gggcgatatc atcacaaagc
    18301 tcaggaacgt attttttatc caccagcaca atcggcgcgg cgggcgtggt aagcaactgg
    18361 cgcgcagcgt ctggtaaacc gtcagccact ggcaacatga ccgccagcgg tttcgccggg
    18421 cgatgtttgc gcgcccgaag tgtcgccacc gcgttactgt tacgtgcatc gcaggcaaga
    18481 tgaaatccgc caatcccttt gatggcgaca atgttgccca tttttaactg tgcgatagcc
    18541 gcctgtaatg ccgcctcttg ttctgcatgt tcaccatgac ttacccattc aagatgtggg
    18601 ccacactccg ggcaggccac cggctgggcg tggaagcgac gatcgagcgg gtcacggtac
    18661 tctttgtcac aagccggaca caacggaaac gccgccatca cggtaaacgg gcggtcgtaa
    18721 ggcatggcgc gaataatggt gaaacgcggg ccgcagtggg tacagttgat aaacggataa
    18781 cgataacgcc gttcgcctgg ggtattcatt tcggcaaggc aagcagggca agtagcggca
    18841 tccgggacaa tttgcgtatt catggcacct cccgcgctct ggcggatggt gaactcagtg
    18901 ggcagttgtg accagatgta cggctcacgc tcgacgctat caatacgcgc cagcggcggg
    18961 cagtgctgat gcaattgaac aagtaacgtt tccgggtctt cccgcagccg gacttctacg
    19021 ccatcgccgt cattacagac atcgccgtga agatttaatt gctgtgccag ctgccagaca
    19081 aacggacgaa aaccgacgcc ctgcactttg ccacgaatac gcagttggac accgcaagat
    19141 gtgttttttg ccattgagtt attcccgcca tcatgaattg cgtaacccgt cctgccggac
    19201 acgactgcgt cgtatccggc agtctcaggt cggcgatacc gccagctccg tattctacga
    19261 atatttccgg gaattccttt gatgccagaa cagttctgta agatttttag aacatcagcg
    19321 ccgtacggcg gcgtttttct gcactcagtt gttcaagttt attacgatcg acacaaatca
    19381 gcgcatgagt cgggcaagcc gccatacacg ccgggccgtc ttcacgatgg ttgcacaggt
    19441 cacatttatt ggcttcggct ttgtcagccc gtacattcag ccccgcgccg ctgttgcgaa
    19501 tcaccggacg taccaccact tccatcgcgc catacgggca agccacaacg caggttttgc
    19561 aaccaatgca acgttcctgc atcacatgaa caaacccttt atcacggctg atagcaccat
    19621 tcgggcagac gttagcgcac ggtgcatctt cacactgacg gcaaactgtc gccgtggaaa
    19681 tgttcacacc tttaatgaca tggatacgcg gtaaaaatgt ttccggggtc agcgatgcac
    19741 agtcctgatt ttcctgatga gaaaccacgc acgccacttc acaggtacag caaccaatac
    19801 atttactcgc gtcagcaatg atgaaacggt tcatcaaatt ctccagcaat gacagttaat
    19861 gcgccgatac attcacaaat catgccagtt ttaattcact gttatttaag gaaattaatt
    19921 tctgtaatgc aggaaaaaag atgtcatcga cactagtgac gatgacatgt gatgacaatg
    19981 tttatcgcga aagagcaatg agtgagtcgc ggcggatcag ttttccgctg aaggttttcg
    20041 gcggtgagaa atccccgcca tcgagcataa aaatcagccg tccaataatt tcctgaatca
    20101 tctcagtcac cggaattttt acgctggaga gcgccggaac gatatagggg gcaattgcga
    20161 tatcatcgaa tccgataact gacacctgct ctggcaccgc tacgccgcgc tcgtgcaacg
    20221 ctttcatcgc acctatcgcc atatcgtcgt tactggcaac taacgcgcta aatttagccc
    20281 cacgttcgag caacgtttct accccttcgg ccccgctggc gggcgtccat ttaccgttag
    20341 tgataagttt ttcattgagc gcaataccat gctgcgccag cgcgtcttta tacccagcaa
    20401 gacgttcaat gctggtgggg gaatccatcg agccggtaag gaaagcaatc tcctgatgtc
    20461 cggcgtttat caactctgcc acggcattaa agctggtctg tttatgatcg caccagacgc
    20521 tatggctgct gtttttgcgc aggcggcgat taagcaccat tatcggctga ctgtgcgcgt
    20581 caatgatgtc atcgatctca tccacgctta aaaaacgcgg gtaaatcata atggcgtcgc
    20641 agcgcaaatc cagcagatac tgaatcgcct ggcgctcttc ttcggcgctg tgtttaccat
    20701 ctgccaatag caactgccgc cctttctctt ccgccattcg cgcggcatga aagagtaatt
    20761 cactaaaata aatgccgtgg taaagcgtgt tggtcactac caaccccagc gtctgagtac
    20821 tcttcgccga cagattgcgc gccagcaagt ttgggcggta accgctctct tctaccgcct
    20881 gaaacacgcg atctttagtc tcctggctga cgtagccatt acctgaaagc acgcgggaaa
    20941 cggtcgcttt tgaaaccccg gcgcgcttcg ccacttccag catcgtcgtc atcattttca
    21001 tccctttaca cgcaatcaac gcagtgtact gcaccgtttg ccgattgtcc ttgcgcaatc
    21061 ggcgggaaaa atattcaggt gaccggtttc acaaatataa aaaatgaaca attaactctc
    21121 ttgcttattt agtgacaact attcatgatt ttgtgaaacc ggtttcctaa ttccgtttca
    21181 gcatcggcat ttttccgtta cgtcgactga taacaactac atctacccta ctgataacag
    21241 gataaaatcc gatggccaaa aattatgcgg cgctggcacg ctcggtgata gcggcactgg
    21301 gcggcgttga taacatctcg gcggtcacgc actgtatgac gcgtttgcgc gttgttatca
    21361 aagatgacca gcttatcgac agcccgacgt taaaaaccat ccccggcgtg ctcggcgtgg
    21421 tacgtagtga caaccagtgt caggtgatta tcggcaatac cgtttcacaa gcctttcagg
    21481 aagtcgtcag cctgctgccg ggagatatgc agcccgcaca gcccgtgggt aaacccaaac
    21541 tcacgctgcg tcgcattggt gcggggatcc tcgatgcgct gatcggcacc atgtcaccgc
    21601 tgatcccggc gattatcggc ggatcgatgg tcaaactgct ggcgatgatc ctcgagatga
    21661 gcggcgtgct gacaaaagga tcgccgacct taaccattct gaatgtgatt ggtgacggtg
    21721 ctttcttctt cctgccgctg atggtcgcgg catctgccgc catcaaattt aaaaccaata
    21781 tgtcgctggc gattgccatc gcgggtgtgc tggtacatcc gagttttatt gaactgatgg
    21841 cgaaagcggc ccagggtgaa catgtcgaat ttgccctgat tccggtcacc gcggtgaaat
    21901 acacctacac agtgatcccg gcgctggtga tgacctggtg cctgtcatat atcgaacgct
    21961 gggtggatcg cattacaccg gcggtgacca aaaacttcct caagccaatg ctgattgtgt
    22021 tgattgccgc accgctggca atcctgctga ttggcccgat tggtatctgg atcggtagcg
    22081 ccatttcggc gctggtttac accattcatg gttatctggg ctggctttca gtcgccatta
    22141 tgggcgcgct gtggcctctg ctggtaatga ccgggatgca ccgcgtcttt acgccaacca
    22201 tcattcagac cattgccgaa accggcaaag aagggatggt catgccgtca gagatcggcg
    22261 ctaacctgtc gctgggcggt tcatcactgg cggtggcgtg gaaaacgaaa aacccggaac
    22321 tgcgccagac ggcgctggct gcggcggcat cagccattat ggcggggatt tccgaaccgg
    22381 cgttatacgg cgtggcgatc cgcctgaaac gtccgcttat cgccagtctt atcagcggtt
    22441 ttatttgcgg cgcggttgcc ggtatggcgg ggcttgccag ccactcaatg gcagcgccag
    22501 ggttatttac cagcgtgcaa ttcttcgatc cggcgaatcc aatgagcatc gtctgggtgt
    22561 tcgcggtcat ggcgctggcg gtggtgctgt cgtttatcct cacactgttg ctcggctttg
    22621 aggatattcc tgttgaggaa gcggctgccg aggcgcgaaa gcatcagagc gtacaaccga
    22681 ccgtcgccaa agaagtaagt cttaattgag gatgaaaatg tcagtatttc cagaaggttt
    22741 tttatggggc ggcgcgcttg ccgccaacca gtctgaaggt gcgttccgtg aaggtggcaa
    22801 agggctgacc actgtcgata tgatcccaca cggcgagcat cgaatggcgg tgaaactggg
    22861 gctggaaaaa cgttttcagt tgcgcgatga cgagttttat cccagccatg aggcgacgga
    22921 tttttatcat cgttataaag aagatatcgc cctgatggca gagatgggat tcaaggtttt
    22981 ccgtacctca attgcctgga gccgtctctt tccgcagggc gatgaaatca cgcccaatca
    23041 gcagggcatt gctttttatc gttctgtctt tgaagagtgt aaaaagtacg gtatcgaacc
    23101 gctggtcacg ttgtgccact tcgatgtgcc gatgcatctg gtcaccgaat atggctcctg
    23161 gcgtaaccgc aagctggtgg agtttttcag ccgctacgcc cggacctgct ttgaagcatt
    23221 tgatggtctg gtgaaatact ggcttacctt caatgaaatc aacattatgt tgcatagccc
    23281 gttctccggc gcgggtctgg tgtttgaaga aggtgaaaat caggatcagg tgaaatatca
    23341 ggccgcgcat caccagctgg ttgccagtgc gctagccacc aaaatcgccc atgaggttaa
    23401 cccgcaaaat caggtgggct gtatgctggc gggcggtaac ttctaccctt acagttgcaa
    23461 gccggaagat gtctgggcgg cgctggagaa agaccgggaa aacctgtttt ttatcgatgt
    23521 gcaggcgcgg ggcacgtatc cggcttactc tgcccgcgta ttccgcgaaa aaggggtaac
    23581 catcgacaaa gcaccgggcg atgatgaaat cctgaaaaac accgtcgatt ttgtctcttt
    23641 cagctattac gcctcgcgct gcgcctcggc ggagatgaac gccaacaata gcagtgcggc
    23701 gaacgtagtg aaatcgctgc gtaacccgta tctgcaagtg agcgactggg gctggggtat
    23761 tgatccactc ggtctgcgta tcaccatgaa catgatgtac gaccgttatc agaagccgct
    23821 gtttctggtg gaaaacggcc tcggcgcaaa agatgaactt gctgccaatg gcgagattaa
    23881 cgatgactat cgcatcagct atttacgcga acatatccgc gcaatgggcg aagcgattgc
    23941 agatggcatt ccgctgatgg gctacaccac ctggggctgt attgatttag tctccgcctc
    24001 tacgggtgaa atgagcaaac gctacggttt tgtctacgta gaccgtgacg acgcaggcaa
    24061 cggcacgctg acgcgcacac gtaagaaatc gttctggtgg tataaaaaag tgattgccag
    24121 taatggggaa gatttagagt aggtaacagt gccggatgcg gcgtgaacgc cttatccggc
    24181 ctacatgtcg cacgaaccag gaaggcccga taagcgcagc gcatcgggca atctggggtt
    24241 tggcatcagt caaaatcaaa gaaaccattc cggcttaacg ccaaatcggg ctgaaagtgc
    24301 cttaatgtgg gatatcgtca gggatcgttg tccagacaaa atctgactga ccagggattt
    24361 agagccgatt tcgttcttca aatccgcata ggtcagatta tgttgatcaa tcagcgtgcg
    24421 aagtaacgcc acgccaacag gcattgccgc aacggctttg tcgaactctg caaactcttg
    24481 gttgttgttt tcatattctg caatgcggct cgccagaaag tcgataagtg gattggtgtc
    24541 atcttcttca atcaaatatt cgaccagttc cagtgcttca cgataatcat cttctgagtc
    24601 actacctccc agaaagggga ctgcattgac cagttctctt gttgctttaa cagcgcgtgc
    24661 agcattagca gtcattcttt gttctctctg tagtaacgtg tcagtttgtc atactcggcg
    24721 tgattagtta tgtgcttcac aaagaaccgc ttattaacaa agtttatgta ggcaatcacc
    24781 cttaggttgt tcccacctac atccagaacg taccatttgt tgcgatattt aaaattgtcg
    24841 aggttgggaa acgccgttcg catctcttct ggcgtagaaa aatccgtttc cttaataacc
    24901 cgatacaatg cctgaagagc cagagcgtcg ttaggatatt tacgcgcaga ctcttcaaat
    24961 ggcgctttag aaatgatgtg catttttaag ttttaacttc cttatagtaa gtaaacagcc
    25021 acatctgcga gttctatcct tatgtgttcg gaaactatag ctcatcaaat ccaagttcac
    25081 aatatgtaaa cacttgagcg cgatataatt acccccaaaa tcctcatagc cactctgcaa
    25141 aaagtcagtt ctggttcacc ccatcaagaa catccccctt tatcaagcaa cgctgcgtct
    25201 gcgccagatc ttccgtttgc gggtcgcgcc cggcgatggg tttgaggacg agtttacggt
    25261 tgggggtata agtaaaacgc gtgagcgcgg gatagcttaa cttgccaaac cagccgtgaa
    25321 cacgctgcca tttggcgtct tttacctgat acagactggt tatccagtaa ccggaatcaa
    25381 actgcatatc cagcaattgc gtgtgaccat tgccttgcaa atccagcaaa tcatctacgc
    25441 cagttttgct tgcggtgtag aatcccctgg gttcaaagac acagggacgg ccttcgcgcg
    25501 tgaacgtcat aagaattaaa tgtgcataag gagccagacc attaccggta cttggtaacc
    25561 agataaccag atcctgaatg ccgttacgat caagatcggc gcgatagagt tgccctttgc
    25621 ccgctatgtt tgccagcata cccacaggca cagaccagga tttaccggcg cgatctttac
    25681 cgttcaattg ccattcacta tcatcatttg gcatcttttc aagcgtcacg ccagcaacgc
    25741 ccacttttct aaaacgccag gtttgctcgg catcataagg catggtcagg agaggtttca
    25801 gcgcccgctt ttgcgcgtat tcgctgtcac tcattggcag atattcggta aagctggtgg
    25861 cctgtgcagg cagcactaag ctcaataaaa aagcgcacat ccctgttctg atattcattt
    25921 cgctgaattc cctgatggag aattattctt cttccaccgc taactgcgcg aagccgccat
    25981 tcccttccca gccttccagt cgttgataaa cggtttctac cgcatcttta atcggctgag
    26041 tcatcgggta gtaaaagccg acgatatccg gctggatgcc gaggaaaatc acttcgccaa
    26101 tgtcttcttt caactggtcg ataagatagt tcagcggcat gttatgggta gtcatcataa
    26161 acatctcggc gatatcatcc gggtcgatga tgcggatttc gccggggttt agccccatat
    26221 cagtagcgtc aacaatcagt aaccgcgtag ggcgcagttc acggatggcg acgatgtcgt
    26281 tttccggtgc actaccgcca tcaatcacca cccagttacc tttcggcgcg gcggcgcact
    26341 tttccgccag cagcgggcca gcgccatcat cgcccatcat gctattgcca acacagagta
    26401 aaacgtcagt cacgcagtct cctcaccatc aaatagatgg cgttttcctg atgaatatca
    26461 tgcagcatac ttaacagcat tgtactccat tccttctgct gcgaagtttg cgctgcccgg
    26521 gctttatcga atgcgttggc cagcatcggc acatggttaa tgtcgatgac gatctcacca
    26581 tatttcggca cgccttccat tttgcggcgg gcttcactgc ccgcctccag agtggcaatc
    26641 catgccagat attcatccca cgggcaggtg agcgccgctt ccaggcaatc gataacccca
    26701 aggtggtgac caatcgccag gctgtaatag accacctgct gcgcctcggc gggcgtggca
    26761 tcgttctcat caataaattt acggctcagt tgactgaaca ccaccttttc actcatcgga
    26821 tacgcgcctc ttcaacaaca tgattcagat ggctgacaat ctcgttcaga cgcggatcgt
    26881 tttccgcttc cagccagcgt gcaacctgtt cttcgccctg ccctaactgt gtcaggaagt
    26941 catcggcaat ctgacgaccg taacgataac ccgccaggcg gcgtgcttcg cgatccactt
    27001 tcacacgcag cggctgcacc atatcgccat gcaggatttc cgcctgttgt tcatcctgct
    27061 cacccgggcc acgggcgtga attttctgct ccagcaggcc gagcgccatt gcaaagccgt
    27121 acagcgtggc ggcaggcgtt ggcgggcaac caggaatgta gacatctacc gggacgattt
    27181 tatccgtacc gccccacacg cagtagagat cgtggaaaat accaccgctg ttaccgcagg
    27241 caccgtagga gatacaaatt ttcgggtccg gcgcggactg ccacgcacgc agcgcagggg
    27301 atcgcattgc acgggtgacc gcgccggtaa acagtaaaat atccgcatga cgcggtgaag
    27361 gaacgacttt aatgccgaag cgttctgcat caaacagcgg cgaaagcgtg gcgaaaattt
    27421 cgatttcgca gccgttgcag ccgccgcagt ccacgcgata aacgtaggca gaacgtttga
    27481 tttttttcag taacgacgcc ttcatgctgg cgatggattc atccaccgtc atcgggaccg
    27541 gaatgccgtt ggcgtcacgg gggcctaata aattgctcat cagatggcct ctttcatatg
    27601 gcgagtcagt tcaatacggt cggacggcac caggcatttc tggcgcttac attccgggca
    27661 agtctcaaag ctttcgcggt ggttttccgc gcggctgtcg ccgttgtgct taagcagcgc
    27721 aatggcgtag tcgatctctt tctggacggc gaaaggacga ttgcagacgc ggcagttgca
    27781 cagcgcgaag cgggactgtt gcaggaagtc ttctttcttc cacactgcca gttcgtactc
    27841 ttgcgacagt ttgatcgccg ccgtcgggca aacttcttcg cagcgtccac agaagatgca
    27901 gcgcccgaga ttaaactccc acgccagctc accggtggcg aggtcagttt caaccgttaa
    27961 ggcgtttgac gggcaggcat tgacgcaggc cgcacagccg atgcactgct gcgggttctg
    28021 ctccggctta ccacggaagt ttttatcaac cgcaatcggc tccagcggat aagacgaggt
    28081 cgccgtgccg gttttgatga cttttttgat aaaggtaaac atggcgattc cttatttcag
    28141 cggcgagttt ttacgctcaa tgctgtaacg ctcgagttct ttgtacggca ccactttgct
    28201 ctttttctta cgcacatcga ccacggtcat gcggtcggta caggagtagc aagggtccaa
    28261 gctaccgata atcagcggcg catcggaaac ggtgttaccg cgcagcatgt agcgcagggt
    28321 cggccagttc gcgtaggtcg cggcacggca gcgccagcgg tacagcttct ggttgtcgcc
    28381 ggtcatgctc cagtggatat cgtcgccgcg cggcgcttcg gcaaagccca gcgcgaaacg
    28441 gtgcggaatg taggtaaagc cttccaccat cagcggaccg cccggcaggt tatccagacc
    28501 gtagtcgatc atgttcagcg cggtataaac ttcgttgata cgcactttca gacgggaaat
    28561 aacgtcgcag ccctgctcgc tgtggacttc cattggcagc aggccataac cgacaaacgg
    28621 gtgatcggcg cgagtatcgc gggcgtgacc gctggcgcga accatcgggc caacgttact
    28681 gaaatcgcgg gccatttccg ggtccagacg accaatgccg acggtgcgct gttccatgtt
    28741 cggcgtactc agcagcacat ccaccagctc ctgcacttca cgacgcatct gttgcgccag
    28801 ctggcgggtc tggatcatgt catctttcaa cagatcgcga cgaatcccgc cgatcaggtt
    28861 caggccgtag gttttacgcg caccggtgag gatctctgcc attttcatgg aggtttcacg
    28921 cacgcggaag aactgcataa agccggagtc aaagccggta aagtggcagg cgagaccgag
    28981 gttgagcaga tgcgagtgca ggcgttccac ctccagcaga atggcgcgga tcatctgcgc
    29041 acgttctggc acctgaatac ccatcgcgtt ttctaccgac gtggtgtagg cggtgctgtg
    29101 ggcaaagccg cagatcccgc acacacggtc agagaggaag gtcacttcgt tgtaacccat
    29161 acgggtttcc gccagttttt ccatgccgcg atgaacgtag aacagacggt agtcggcgtc
    29221 gataatgttt tcgccatcga cgaacagacg gaagtggccc ggttcgtcag aagtgacgtg
    29281 cagcggacca atcggcacga cgttgttttt cttgtcgccc agttcgttga tgaactcgta
    29341 ggtttcagca tcggtggtcg gtgccggacg ctgacgataa tccatgctgt ctttacgcag
    29401 cggataaagt tcatccggca gcaccagacg gcgttcatcc ggcagaccaa ccggaatcaa
    29461 accgtacata tcgcgcactt cgcgctcgcc ccacaccgcc gccggaacgc gcggcgtcac
    29521 ggacggatac tccggtttgt tggcatcaac ttcgacgcga accgttaccc agcacttagt
    29581 ccccttctcc atcgacagca cgtaataaac ggcgtaatga ccattcagtt tgcgttcgtc
    29641 gttaccaaaa agcaccgaca gccagccccc ctgcttgtag tagagaaact ccaccacttc
    29701 cggcagatag ttcaccttta cggtgacagt cagctgatct ttggtctgcc aggcgtggtc
    29761 cagcacgacg cccggaaatg cctcattcag cgcggcgaga taatgttgac ctaatttttc
    29821 ttcagacatg ctcaaactct ctttaatcac gccgccagca aggagacgaa cgctaaaaat
    29881 gcaaagccaa acccagtcca ggtaatgcgc ggagtaatat caagacgcag acgggccatg
    29941 ctgttttcga acagcgcgat aaccaggacg ccgacgacca gtttgacgat ggcaatcacc
    30001 agcgccagca gcagcccacc ggcggtgaag gtttccatct gtccccacgg gataaatacg
    30061 ccgacgaaca tctgcagcac caccagctgt ttcaggctga taccccattt catgacgcca
    30121 aagccgctgc cgctgtattc agagagcggg ccttcctgca gctcctgctc ggcttccgcc
    30181 aggtcgaacg gcagtttgcc catttcgata aaggtggcga acgcacaggc acaaagcgcc
    30241 agtaccagcg ggatgctctg gctcagcggc cagtgataaa cggtgtcggt gatgttgctg
    30301 atgttggtgg aaccggcaac ctgtgcggca acccacagac caagcagcag catcggttcg
    30361 accagcacgc caagcatcgc ttcacggctc gcgccgatag cggtaaacgg gctaccggta
    30421 tccagaccag aaatggcaaa gaagaaacgc gcgatggcaa agagatacag taaggtgatt
    30481 aaatcaccca gttgcggcag cggagaaccg acggtcacca ccggcagcgc agtagcgata
    30541 gtcagcatga cgcccaccat cacatacggc gtcaggcgga acacccagcc ggaggcatcc
    30601 gggccaacgc tctggcggcc cagcagtttg aaaatgtcac gatactcctg caacacaccc
    30661 ggcccacgac ggttatgcag acgggcgcgc gccacgcggg taataccgga gagcagcggc
    30721 gcaacggcaa ataacaccag cgcctgaatt aacggatata aaacactcat tctcaggctc
    30781 ctcgtgaaac aataatcacc accagtaccg ccagttcaac cagcgccatc cggcggaaca
    30841 gcaacgcact accttcgcac tgccagcccg gcaccagcga caccggattc agccatttgc
    30901 gcagtttcag cactggcgca aacgcctgtt taaccggcat ggcaaaaccg tgagcggtaa
    30961 tcaccattga tttttcatgg tcgtagccgc acacccaggc cgcaccgcgg gaacgtgacg
    31021 gcaggcggtc gcctttgcaa atcgccataa tgatgaatgg cagcagcggg caggcaatca
    31081 gcagcaatgt gatcattggt tgagaaacgg tggtgttagc aggctccaga ggcagaggta
    31141 cagcagcaga gagcatcggc agcagccacg gcgcagcaac accgccaatt acgcagcaaa
    31201 tcgccagtgc cactacgctt acgctcatca ggagcggcgc acaggtggcg ttttcggctt
    31261 ctttggtacg cggcgcgccg aggaacgtga cgccatagac tttcgccata cacatcaccg
    31321 ccagcgcacc ggtaattgcc agacccacag cgagcagcgg ccccagcaga cgggcaacaa
    31381 acgcgccgct attgctcagt ttgaagaagg attgatagat aacccattcc ccggcaaacc
    31441 cgttcagtgg cggcagcgca gccattgcca tcaacccgac taacatggcg atggagataa
    31501 ccggcatttt cttgccaata ccaccgagtt tttcgatatc gcgatgaccg gtacggaacc
    31561 agacgctccc cgccccgagg aacagtacgc ttttgaacag gctatggtta agcagatggt
    31621 acagaccacc gaccaggcca agagcaatca gcgccggttg ttcgagcgcg atacccgtta
    31681 cgccagcgcc cagccccagc aggataatgc cgatattttc cagggtgtgg taagccagca
    31741 ggcgctggat gttgtgttcc atcaacgcat acagaccacc gacgaacgcg gtgatcatgc
    31801 cgagcaccag cagcgcgatc ccccaccaca gcggtgcatt accgcccagc agtgacaggg
    31861 ttaacatgcc cagcaggcca attttcatga ctaccgtaga aaacaacgcg gcagctggcg
    31921 cagaggcgtt agcatgtgcc tgcggcaccc agccgtgcag cggaataatc ccggccagca
    31981 ggccaaagcc aatcacaccg agcagccaga tatcggaacc gagcggcagc tgttgcatac
    32041 gcatatccag taggcacaga tccagcgtgc cataacgctg ccacagcagc cagcaagcaa
    32101 tcaccagcag cagagtgcca agacgcccca gcgcaaacca cagtttgccc tctttgctgt
    32161 tgctggtgag gaacaccgca cacaaggcca tgacttcggc cattaccacg aacatgccga
    32221 ggttgctggc gatgacggcg cagacggcgg cagccatcaa catattgatc tgcaagccgt
    32281 tgcacttcac ctgcgcgtgg cgatgccagt caatgttgta gaggctgaca aacagaccgc
    32341 acagaccgag cgtaatcagc caaatcgcgt taagcggaga gatttgcaca tcgtagctta
    32401 ccagcgacag cgcaccgctc acgccaaccg cgccagtcag tacagtgaag cccgcggctg
    32461 ccgtatacag actaccaacc gcgccgccaa ttccagctat ccagccactt aacgcttttt
    32521 gaaaagaaaa gagaaatgcc agaacagcgg cggcgacaaa ccatgccacg ccgctattga
    32581 tcagggaaat tgcgctcatt tagcctctcc actttgagcc tgctgaaaca aggtgagatc
    32641 gccaaagtcc gtgttaaagg tcagctcacg cttacgtttg ctgacgcggg cgatatcggt
    32701 gttatccacc agatgcaggg ctttagtcgg gcacatccgc acgcaggccg gaccttgttc
    32761 atcaaaactg caaaggtcac atttgacggc gatcgcgcga atacccggca cccagtcaag
    32821 cagcgtactg atacgcgccg gagcaggcgg tgccggtggc gctttcgggg tattggcgtt
    32881 tgccggaata tccagcggac ggctgccgga aaattcaatt gcgccaaacg ggcaggcgat
    32941 gccgcacagt ttgcagctta cgcacagact ttcattcaac tgcacggccc catcaatgcg
    33001 ggtgatggcg ttaaccgggc aaaccaccgc acagggtgca tcttcacagt ggtgacagag
    33061 ctgcggcgca gattcttttt cattcagcat cactctcagg cgcggcattg attgcaggcc
    33121 gtgctggcga tgcgtctctg aacaggcggc ctcacaagtg tggcagccga tacagagcgt
    33181 ggagtcaaca attacaaaac gattcaccag gcattcctca ggtgattgtc atttttgacg
    33241 aaaacatgcc gatgaaatgt cattttcgac actcatcgac acgcccatcc ccaaacaggc
    33301 gtaacgcctg caaaacgggc aaagcctcag ctcatgctgc cgggctttgt ccctttacca
    33361 gttggcttaa attcaccggc acattgtttt caatagcggt gtataagctg tcgtaaacgc
    33421 cagcgatttt ttcgagatag tgttccagaa cctgttcgcg atcgcggatg ttgaccttgc
    33481 catcaatcat gccgtcaatg ctcagttgcg cctgcgcaat ccgctgtttg tcttcgccat
    33541 cttttgtttc gacgtaatac tcgatggtgt ggctgttaaa gccatccgcc agggtgacgt
    33601 aaatacgaaa atgttcaaaa aggacgaaac agagttcttt gtccggcgcg cagctcatgg
    33661 cgtgatgcag gcgcgctttc gataacgtga tcccctgaac cagcgaattg cagtagatgt
    33721 gccactggtc ctgtaggcga caatgccgct gtgcgatgta atcggctttc tcgcttattt
    33781 cccaaatagt cattgtcagg ttacccgttt aacagagatg ccagctttaa gcattttcta
    33841 tgccaacttt taatttattg ttattaaata gatttttaag ctaaagatga atttcgtcgc
    33901 cgtgtcgacg tgtcatttcg acatcatcga cattattcac cgcagggata atcaacactg
    33961 gcacaattat tgcttgtagc tggcaatagt taatgggagg cgatatgcac gaaataaccc
    34021 tctgccaacg ggcactggaa ttgatcgaac agcaggccgc aaaacacggc gcaaaacgcg
    34081 taactggggt ctggctcaaa attggcgcat tttcttgtgt cgaaaccagc tctcttgcct
    34141 tttgttttga tctggtttgc cgcggcagcg tggcggaagg ttgtaaactg cacctcgaag
    34201 aacaagaggc cgaatgctgg tgtgaaacat gccaacagta tgtgacgcta ctgacccagc
    34261 gcgtccgccg ctgtccacag tgtcatggtg acatgctaca gattgtggca gacgacggtt
    34321 tacagattcg gcggatagaa atagaccagg agtgagcgat gtgtacaaca tgcggttgcg
    34381 gtgaaggcaa cctgtatatc gagggtgatg aacataaccc tcattccgcg tttcgtagcg
    34441 cgccatttgc cccggcggca cgcccgaaga tgaaaatcac cggcattaaa gcgcctgaat
    34501 ttacccccag ccagactgaa gaaggcgacc tgcattacgg tcatggcgaa gcgggcactc
    34561 acgcgccggg catgagccag cgtcggatgc tggaagtcga aattgacgtg ctggacaaaa
    34621 ataaccgtct ggctgaacgc aaccgcgcgc gctttgctgc ccgcaagcaa ctggtgctca
    34681 acctggtttc cagccctggt tccggtaaaa ccaccctgct gacggaaacc ctaatgcgcc
    34741 tgaaagacag cgttccgtgc gcggttattg aaggtgacca gcaaaccgtg aacgatgccg
    34801 cacgcattcg cgctaccggc acaccagcga ttcaggtgaa caccggtaaa ggctgccatc
    34861 ttgacgcaca gatgattgcc gacgccgcac cgcgtctgcc actggacgat aacggtattc
    34921 tgtttatcga aaacgttggc aacctcgtat gcccggccag cttcgatctc ggtgaaaaac
    34981 acaaagtggc ggtgctttcc gttaccgaag gtgaagacaa accgctgaaa tatccgcata
    35041 tgtttgccgc cgcctcgctg atgctgctca acaaagttga cctgttgccg tatctcaact
    35101 ttgacgttga gaagtgcatc gcctgcgccc gcgaagtcaa tccagaaatt gaaatcatcc
    35161 ttatttccgc caccagcggc gaagggatgg accagtggct gaactggctg gagacacagc
    35221 gatgtgcata ggcgttcccg gccagatccg caccattgac ggcaaccagg caaaagtcga
    35281 cgtctgcggc attcagcgcg atgtcgattt aacgttagtc ggcagctgcg atgaaaacgg
    35341 tcagccgcgc gtgggccagt gggtactggt acacgttggc tttgccatga gcgtaattaa
    35401 tgaagccgaa gcacgcgaca ctctcgacgc cttacaaaac atgtttgacg ttgagccgga
    35461 tgtcggcgcg ctgttgtatg gcgaggaaaa ataatgcgtt ttgttgatga atatcgcgcg
    35521 ccggaacagg tgatgcagtt aattgagcat ctgcgcgaac gtgcttcaca tctctcttac
    35581 accgccgaac gccctctgcg gattatggaa gtgtgcggtg gtcataccca cgccattttt
    35641 aaattcggcc tcgaccagtt actgcctgaa aacgttgagt ttatccacgg tccgggttgc
    35701 ccggtgtgcg tactgccgat gggtagaatc gacacctgcg tggagattgc cagccatccg
    35761 gaagtcatct tctgtacctt tggcgacgcc atgcgcgtgc cggggaaaca gggatcgctg
    35821 ttgcaggcaa aagcacgcgg tgccgatgtg cgcatcgttt actcgccgat ggatgcgttg
    35881 aaactggcgc aggagaatcc aacccgtaaa gtcgtattct tcggcttagg ttttgaaacc
    35941 accatgccga ccaccgccat caccctgcaa caggcgaaag cccgcgatgt gcagaatttt
    36001 tacttcttct gccagcatat tacgcttatc ccgacgctgc gcagtttgct ggaacagccg
    36061 gataacggta tcgacgcgtt cctcgcgccg ggccacgtta gtatggttat cggcactgat
    36121 gcctataatt ttatcgccag cgattttcat cgtccgctgg tggtcgctgg tttcgaaccc
    36181 cttgatctac tgcaaggcgt ggtcatgctg gtggagcaga aaatagcggc ccacagcaag
    36241 gtagagaatc agtatcgtcg ggtggtaccg gatgccggta acctgctggc gcaacaggca
    36301 attgctgatg tgttctgtgt caacggcgac agcgaatggc gcggcttagg cgtgattgaa
    36361 tcttctggcg tgcacctgac gccggattat caacgattcg atgccgaagc acatttccgc
    36421 ccagcaccgc agcaggtctg cgatgacccg cgcgcgcgtt gtggtgaggt attaacgggc
    36481 aaatgtaagc cgcatcaatg cccgctgttt ggtaacacct gtaatcctca aaccgcattt
    36541 ggtgcactga tggtctcctc cgaaggagcg tgcgccgcgt ggtatcagta tcgtcagcag
    36601 gagaatgaag cgtgaataat atccaactcg cccacggcag cggcggccag gcgatgcagc
    36661 aattaatcaa cagcctgttt atggaagcct ttgccaaccc gtggctggca gagcaggaag
    36721 accaggcacg tcttgatctg gcgcagctgg tagcagaagg cgaccgtctg gcgttctcca
    36781 ccgacagtta cgtcattgac ccgctgttct ttcctggcgg taatatcggc aagctggcga
    36841 tttgcggcac agccaatgac gttgcggtca gtggcgctat tccgcgctat ctctcctgtg
    36901 gctttatcct cgaagaagga ttgccgatgg agacactgaa agccgtagtg accagcatgg
    36961 cagaaaccgc gcgcgcggca ggcattgcca tcgttactgg cgatactaaa gtggtgcagc
    37021 gcggcgcggc agataaactg tttatcaaca ccgctggcat gggcgcaatt ccggcgaata
    37081 ttcactgggg cgcacagacg ctaaccgcag gcgatgtatt actggttagc ggcacgctcg
    37141 gggaccacgg ggcgactatc cttaacctgc gtgagcagct ggggctggat ggcgaactgg
    37201 tcagcgactg cgcggtactg acgccgctta ttcagacgct gcgtgacatt cccggcgtga
    37261 aagcgctgcg tgatgccacc cgtggtggtg tgaacgcggt agttcatgag ttcgcggcag
    37321 cctgcggttg tggtattgaa atttcagaag tggcactgcc ggttaaacct gccgtgcgcg
    37381 gcgtttgcga actgctggga ctggacgccc ttaactttgc caacgaaggc aaactggtga
    37441 tcgccgttga acgcaacgcg gcagagcaag tgctggcagc gttacattcc catccactgg
    37501 ggaaagacgc ggcgctaatt ggtgaagtgg tggaacgtaa aggtgttcgt cttgccggtc
    37561 tgtatggtgt gaaacgaacc ctcgatttac cacacgccga acctcttccg cgtatatgct
    37621 aataaaattc taaatctcct atagttagtc aatgaccttt tgcaccgctt tgcggtgctt
    37681 tcctggaaga acaaaatgtc atatacaccg atgagtgatc tcggacagca agggttgttc
    37741 gacatcactc ggacactatt gcagcagccc gatctggcct cgctgtgtga ggctctttcg
    37801 caactggtaa agcgttctgc gctcgccgac aacgcggcta ttgtgttgtg gcaagcgcag
    37861 actcaacgtg cgtcttatta cgcatcgcgt gaaaaagaca ccccattaaa tatgaagacg
    37921 aaactgttct ggcacacggt ccggtacgcc gcattttgtc gcgccctgat acgctgcatt
    37981 gcagttacga agaattttgt gaaacctggc cgcagctggt cgcaggtggg ctatacccca
    38041 aatttggtca ctattgcctg atgccactgg cggcggaagg gcatattttt ggtggctgtg
    38101 aatttattcg ttatgacgat cgtccctgga gcgaaaaaga gttcaatcgt ctgcaaacat
    38161 ttacgcagat cgtttctgtc gtcaccgaac aaatccagag ccgcgtcgtc aacaatgtcg
    38221 actatgagtt gttatgccgg gagcgcgata acttccgcat cctggttgcc attaccaacg
    38281 cggtgctttc ccgcctggat atggacgaac tggtcagcga agtcgccaaa gaaatccatt
    38341 actatttcga cattgacgat atcagtatcg tcttacgcag ccaccgtaaa aacaaactca
    38401 acatctactc cactcactat cttgataaac aacatcccgc ccacgaacag agcgaagtcg
    38461 atgaagccgg aaccctcacc gaacgcgtgt tcaaaagtaa agagatgctg ctgatcaatc
    38521 tccacgagcg ggacgattta gccccctatg aacgcatgtt gttcgacacc tagggcaacc
    38581 agattcaaac cttgtgcctg ttaccgctga tgtctggcga caccatgctg ggcgtgctga
    38641 aactggcgca atgcgaagag aaagtgttta ccactaccaa tctgaatttg ctgcgccaga
    38701 ttgccgaacg tgtggcaatc gctgtcgata acgccctcgc ctatcaggaa atccatcgtc
    38761 tgaaagaacg gctggttgat gaaaacctcg ccctgaccga gcagctcaac aatgttgata
    38821 gtgaatttgg cgagattatt ggccgcagcg aagccatgta cagcgtgctt aaacaagttg
    38881 aaatggtggc gcaaagtgac agtaccgtgc tgatcctcgg tgaaactggc acgggtaaag
    38941 agctgattgc ccgcgcgatc cataatctca gtgggcgtaa taatcgccgc atggtcaaaa
    39001 tgaactgcgc ggcgatgcct gccggattgc tggaaagcga tctgtttggt catgagcgtg
    39061 gggcttttac cggtgccagc gcccagcgca ttggtcgttt tgaactggcg gataaaagct
    39121 ctctgttcct cgacgaagtg ggcgatatgc cactggagtt acagccgaag ttgctgcgtg
    39181 tattgcagga acaggagttt gaacgtctcg gcagcaacaa aatcattcag acggacgtac
    39241 gtttaattgc cgcgactaac cgcgatctga aaaaaatggt cgccgaccga gagttccgta
    39301 gcgatctcta ttaccgcctg aacgtattcc cgattcacct gccgccacta cgcgagcgtc
    39361 cggaagatat tccgctgctg gcgaaagcct ttaccttcaa aattgcccgt cgtctggggc
    39421 gcaatatcga cagcattcct gccgagacgc tgcgcacctt gagcaacatg gagtggccgg
    39481 gtaacgtacg cgaactggaa aacgtcattg agcgcgcggt attgctaaca cgcggtaacg
    39541 tgctgcagct gtcattgcca gatattgttt taccggaacc tgaaacgccg cctgccgcaa
    39601 cggttgtcgc ccaggagggc gaagatgaat atcagttgat tgtgcgcgtg ctgaaagaaa
    39661 ccaacggcgt ggttgccggg cctaaaggcg ctgcgcaacg tctggggctg aaacgcacga
    39721 ccctgctgtc acggatgaag cggctgggaa ttgataaatc ggcattgatt taactgcaaa
    39781 ttgccggaca gatctgcctg tccggcatac tattcacgag gttttttcgg acgatatttt
    39841 tccggcagtt ctggtaccgg acgcttgtca tcgatgagat gacgcacggt taagatcgga
    39901 tgacgccaca gcattctcgg cccggcccaa cgcataatct gtttcatctc ttcacgcttt
    39961 gcaggctggt aacagtgcac cggacactgc ttacaggctg gtttctcttc gccgaacaca
    40021 catttatcca gccgcttttg cgcgtaaaca aacaacgcct cgtaatgctc cggctccgct
    40081 gacgcctgcg ggcatttcgc ttgataaaga tcgatcattt ttttaatcgt cagtttttca
    40141 cgagagatac gcttgccgga catgctgcct ccatactctt aaggtgtatt tatattacaa
    40201 cttaatttga aaggggacta tggctctgaa ggaggaggtt tattcaaccg acgcaatttt
    40261 gcgtacttgc ttcataagca tcacgcaaaa gctgcaaaac agcatctttc ccggaaccag
    40321 catcaagaac tcgccgttcg cttcttcccc tgaaatgatt aactccggta tcatgtgcgc
    40381 cttatgtgat tacaacgaaa ataaaaacca tcacacccca tttaatatca gggaaccgga
    40441 cataacccca tgagtgcaat agaaaatttc gacgcccata cgcccatgat gcagcagtat
    40501 ctcaggctga aagcccagca tcccgagatc ctgctgtttt accggatggg tgatttttat
    40561 gaactgtttt atgacgacgc aaaacgcgcg tcgcaactgc tggatatttc actgaccaaa
    40621 cgcggtgctt cggcgggaga gccgatcccg atggcgggga ttccctacca tgcggtggaa
    40681 aactatctcg ccaaactggt gaatcaggga gagtccgttg ccatctgcga acaaattggc
    40741 gatccggcga ccagcaaagg tccggttgag cgcaaagttg tgcgtatcgt tacgccaggc
    40801 accatcagcg atgaagccct gttacaggag cgtcaggaca acctgctggc ggctatctgg
    40861 caggacagca aaggtttcgg ctacgcgacg ctggatatca gctccgggcg ttttcgcctg
    40921 agcgaaccgg ccgaccgcga aacgatggcg gcagagctgc aacgcactaa tccggcggaa
    40981 ctgctgtatg cagaagattt cgccgagatg tcgctgattg aaggccgtcg cggcctgcgc
    41041 cgtcgcccgc tgtgggagtt tgaaatcgac accgcgcgcc agcagttgaa tctgcaattt
    41101 ggcacccgcg atctggtcgg ttttggcgtc gagaacgcgc cgcgcggact ttgtgctgcc
    41161 ggttgtctgt tgcagtatgc gaaagatacc caacgcacga ccctgccgca tattcgttct
    41221 atcaccatgg aacgtgagca ggacagcatc attatggatg ccgcgacacg tcgtaacctg
    41281 gaaattactc agaacctggc gggcggtgcg gaaaatacgc tggcttctgt gctcgactgc
    41341 accgtcacgc cgatgggtag tcgtatgctg aaacgctggc tgcatatgcc agtgcgcgat
    41401 acccgcgtgt tgcttgagcg ccagcaaact attggcgcat tgcaggattt caccgccgag
    41461 ttgcagccgg tactgcgtca ggtcggcgac ctggaacgta ttctggcacg tctggcttta
    41521 cgaactgctc gcccacgcga tctggcccgt atgcgccacg ctttccagca actgccggag
    41581 ctgcgtgcgc agttagaaac tgtcgatagt gcaccggtac aggcgctacg tgagaagatg
    41641 ggcgagtttg ccgagctgcg cgatctgctg gagcgagcaa tcatcgacac accgccggtg
    41701 ctggtacgcg acggtggtgt tatcgcatcg ggctataacg aagagctgga tgagtggcgc
    41761 gcgctggctg acggcgcgac cgattatctg gagcgtctgg aagtccgcga gcgtgaacgt
    41821 accggcctgg acacgctgaa agttggcttt aatgcggtgc acggctacta cattcaaatc
    41881 agccgtgggc aaagccatct ggcacccatc aactacatgc gtcgccagac gctgaaaaac
    41941 gccgagcgct acatcattcc agagctaaaa gagtacgaag ataaagttct cacctcaaaa
    42001 ggcaaagcac tggcactgga aaaacagctt tatgaagagt tgttcgacct gctgttgccg
    42061 catctggaag cgttgcaaca gagcgcgagc gcgctggcgg aactcgacgt gctggtgaac
    42121 ctggcggaac gggcctatac cctgaactac acctgcccga ccttcattga taaaccgggc
    42181 attcgcatta ccgaaggtcg ccatccggta gttgaacaag tactgaatga gccatttatc
    42241 gctaacccgc tgaatctgtc gccgcagcgc cgtatgttga tcatcaccgg tccgaacatg
    42301 ggcggtaaaa gtacctatat gcgccagacc gcactgattg cgctgatggc ctacatcggc
    42361 agctatgtac cggcacaaaa agtcgagatt ggacctatcg atcgcatctt tacccgcgta
    42421 ggcgcggcag atgacctggc ttccgggcgc tcaaccttta tggtggagat gactgaaacc
    42481 gccaatattt tacataacgc caccgaatac agtctggtgt taatggatga gatcgggcgt
    42541 ggaacgtcca cctacgatgg tctgtcgctg gcgtgggcgt gcgcagaaaa tctggcgaat
    42601 aagattaagg cattgacgct gtttgccacc cactatttcg agctgaccca gctaccggag
    42661 aaaatggaag gcgtcgccaa cgtacatctc gatgcgctgg agcacggcga cactatcgcc
    42721 tttatgcaca gcgtgcagga cggcgcggcg agcaaaagct acggtctggc ggttgctgct
    42781 ctggcgggcg taccgaaaga ggttattaag cgcgcacggc aaaaactgcg tgagctggaa
    42841 agcatttcgc cgaacgccgc cgcgacgcaa gtggatggta cgcagatgtc tttgctgtca
    42901 gtgccagaag aaacttcgcc tgcggtcgaa gcactggaaa atctcgaccc ggattcactg
    42961 actccgcgtc aggcgctgga gtggatttat cgcttgaaga gtctggtgta ataacaattc
    43021 ccgatagtct tttgctatcg ggaatattaa cgacaactga cgaatcaaat aaaaataccc
    43081 tgtataatag gaaagcttat tttacagggt aaaaccatgc catctacacg ctatcaaaaa
    43141 atcaatgccc atcactatcg ccatatatgg gtcgttggtg atattcatgg tgaatatcag
    43201 ttattacaat cccgttaaca tcaactctct ttttaccctg aaaccgactt acttatttcc
    43261 acaggcgata atattgatcg tgggccgaag agtcttaacg tgctgcgcct gctaaaccaa
    43321 ccctggttta cgtcggttaa aggcaaccac gaagcgatgg cgttggatgc attcgaaact
    43381 ggcgatggaa atatgtggct tgccagcggt ggtgactggt ttttcgattt aaatgattca
    43441 gagcaacaag aggcaataga tctgttgctg aaattccatc accttccaca tattattgaa
    43501 atcattaatg acaacataaa atatgccat
//
LOCUS       DAJTZL010000010        41364 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.010, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000010 DAJTZL010000000
VERSION     DAJTZL010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 41364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41364
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.010"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..664
                     /locus_tag="OO544_000514"
     CDS             <1..664
                     /locus_tag="OO544_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HCR6766202.1"
                     /translation="SQAITNGFQRSMFSNEAGMGSTPNAAAAAASWPPHPAAQGIVQM
                     IGIFIDTLVICTASAMLILLAGNDTTYMPLEGIQLIQKAMRVLMGSWGAEFVTLVVIL
                     FAFSSIVANYIYAENNLFFLRLNNPKAIWCLRICTFATVIGGTLLSLPLMWQLADIIM
                     ACMAITNLTAILLLSPVVHTIASDYLRQRKLGVRPVFDPLRYPDIGRQLSPDAWDDVS
                     QE"
     gene            734..1510
                     /gene="yaaA"
                     /locus_tag="OO544_000515"
     CDS             734..1510
                     /gene="yaaA"
                     /locus_tag="OO544_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HCR6766203.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQHLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPENARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
     gene            complement(1663..1959)
                     /locus_tag="OO544_000516"
     CDS             complement(1663..1959)
                     /locus_tag="OO544_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HCR6766204.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(2173..3459)
                     /gene="thrC"
                     /locus_tag="OO544_000517"
     CDS             complement(2173..3459)
                     /gene="thrC"
                     /locus_tag="OO544_000517"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HCR6766205.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALCDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(3460..4392)
                     /gene="thrB"
                     /locus_tag="OO544_000518"
     CDS             complement(3460..4392)
                     /gene="thrB"
                     /locus_tag="OO544_000518"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HCR6766206.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCLELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(4394..6856)
                     /gene="thrA"
                     /locus_tag="OO544_000519"
     CDS             complement(4394..6856)
                     /gene="thrA"
                     /locus_tag="OO544_000519"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HCR6766207.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEGELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     MERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTSVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELVKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(6883..7002)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(6938..7003)
                     /gene="thrL"
                     /locus_tag="OO544_000520"
     CDS             complement(6938..7003)
                     /gene="thrL"
                     /locus_tag="OO544_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HCR6766208.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(7217..7903)
                     /locus_tag="OO544_000521"
     CDS             complement(7217..7903)
                     /locus_tag="OO544_000521"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HCR6766209.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDVIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(8303..8443)
                     /locus_tag="OO544_000522"
     CDS             complement(8303..8443)
                     /locus_tag="OO544_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6766210.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            8539..9255
                     /gene="arcA"
                     /locus_tag="OO544_000523"
     CDS             8539..9255
                     /gene="arcA"
                     /locus_tag="OO544_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HCR6766211.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(9315..10667)
                     /gene="creD"
                     /locus_tag="OO544_000524"
     CDS             complement(9315..10667)
                     /gene="creD"
                     /locus_tag="OO544_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HCR6766212.1"
                     /translation="MLKSSLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELDAPNITFGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLKLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFL
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSVDSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(10725..12149)
                     /gene="creC"
                     /locus_tag="OO544_000525"
     CDS             complement(10725..12149)
                     /gene="creC"
                     /locus_tag="OO544_000525"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HCR6766213.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVTPTEVNVAAEPALLDQALGNLLDNAIDFTPESGRITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(12149..12838)
                     /gene="creB"
                     /locus_tag="OO544_000526"
     CDS             complement(12149..12838)
                     /gene="creB"
                     /locus_tag="OO544_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HCR6766214.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSSPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(12851..13324)
                     /gene="creA"
                     /locus_tag="OO544_000527"
     CDS             complement(12851..13324)
                     /gene="creA"
                     /locus_tag="OO544_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HCR6766215.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            13535..14404
                     /gene="robA"
                     /locus_tag="OO544_000528"
     CDS             13535..14404
                     /gene="robA"
                     /locus_tag="OO544_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HCR6766216.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(14401..15048)
                     /gene="gpmB"
                     /locus_tag="OO544_000529"
     CDS             complement(14401..15048)
                     /gene="gpmB"
                     /locus_tag="OO544_000529"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HCR6766217.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            15100..15621
                     /gene="yjjX"
                     /locus_tag="OO544_000530"
     CDS             15100..15621
                     /gene="yjjX"
                     /locus_tag="OO544_000530"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HCR6766218.1"
                     /translation="MHQVICATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENTSQRGEARSA
                     TLPLPAVILQKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVYQAL"
     gene            complement(15706..16032)
                     /gene="trpR"
                     /locus_tag="OO544_000531"
     CDS             complement(15706..16032)
                     /gene="trpR"
                     /locus_tag="OO544_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HCR6766219.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(16122..18059)
                     /gene="sltY"
                     /locus_tag="OO544_000532"
     CDS             complement(16122..18059)
                     /gene="sltY"
                     /locus_tag="OO544_000532"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HCR6766220.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQVQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDVIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQVDLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            18270..19937
                     /gene="ettA"
                     /locus_tag="OO544_000533"
     CDS             18270..19937
                     /gene="ettA"
                     /locus_tag="OO544_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HCR6766221.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(20244..21476)
                     /gene="nadR"
                     /locus_tag="OO544_000534"
     CDS             complement(20244..21476)
                     /gene="nadR"
                     /locus_tag="OO544_000534"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HCR6766222.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIDTVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(21497..22879)
                     /gene="radA"
                     /locus_tag="OO544_000535"
     CDS             complement(21497..22879)
                     /gene="radA"
                     /locus_tag="OO544_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HCR6766223.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(22928..23896)
                     /gene="serB"
                     /locus_tag="OO544_000536"
     CDS             complement(22928..23896)
                     /gene="serB"
                     /locus_tag="OO544_000536"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HCR6766224.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            24002..24646
                     /locus_tag="OO544_000537"
     CDS             24002..24646
                     /locus_tag="OO544_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HCR6766225.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            24674..25690
                     /gene="lplA"
                     /locus_tag="OO544_000538"
     CDS             24674..25690
                     /gene="lplA"
                     /locus_tag="OO544_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HCR6766226.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTVEGDRKVSGSAYRETKDRGFHHGTLLLNADLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEAITKAFFAHYGERVEA
                     EIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFSWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPDQEKELRELSTWIA
                     GAVR"
     gene            25722..25871
                     /locus_tag="OO544_000539"
     CDS             25722..25871
                     /locus_tag="OO544_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6766227.1"
                     /translation="MIDLENQEREIINLMLSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLK"
     gene            complement(26146..26865)
                     /gene="deoD"
                     /locus_tag="OO544_000540"
     CDS             complement(26146..26865)
                     /gene="deoD"
                     /locus_tag="OO544_000540"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCR6766228.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGIDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(26945..28168)
                     /gene="deoB"
                     /locus_tag="OO544_000541"
     CDS             complement(26945..28168)
                     /gene="deoB"
                     /locus_tag="OO544_000541"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCR6766229.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAVGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(28220..29542)
                     /gene="deoA"
                     /locus_tag="OO544_000542"
     CDS             complement(28220..29542)
                     /gene="deoA"
                     /locus_tag="OO544_000542"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HCR6766230.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            complement(29669..30448)
                     /gene="deoC"
                     /locus_tag="OO544_000543"
     CDS             complement(29669..30448)
                     /gene="deoC"
                     /locus_tag="OO544_000543"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HCR6766231.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            30706..32256
                     /locus_tag="OO544_000544"
     CDS             30706..32256
                     /locus_tag="OO544_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HCR6766232.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQDEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAELKQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIAN
                     GPVHDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYC
                     QQQIAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCKKEG
                     IAARYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGSSSDIGTTPGAR
                     LPYGDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMRE
                     FTANVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISH
                     EQQMRFSQ"
     gene            32228..33091
                     /locus_tag="OO544_000545"
     CDS             32228..33091
                     /locus_tag="OO544_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HCR6766233.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKRCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQHLTGRDNQQIKRSICLLAERGKLAELRLLVIPDQ
                     VDYLHHIDELATFIKRLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(33506..34288)
                     /locus_tag="OO544_000546"
     CDS             complement(33506..34288)
                     /locus_tag="OO544_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR6766234.1"
                     /translation="MICRFIDTHCHFDFPPFSGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAEKYQPLYAALGLHPGMLEKHSDVSLDQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRIHDKLAMHLKRHDLPCTGVVHGFS
                     GSLQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAVRVFDVLCELRPEPEDEIAEVLLNNTYALFSVSG"
     gene            complement(34285..35358)
                     /locus_tag="OO544_000547"
     CDS             complement(34285..35358)
                     /locus_tag="OO544_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HCR6766235.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            complement(35480..35641)
                     /locus_tag="OO544_000548"
     CDS             complement(35480..35641)
                     /locus_tag="OO544_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HCR6766236.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(35768..36373)
                     /gene="osmY"
                     /locus_tag="OO544_000549"
     CDS             complement(35768..36373)
                     /gene="osmY"
                     /locus_tag="OO544_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HCR6766237.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(36766..38355)
                     /gene="prfC"
                     /locus_tag="OO544_000550"
     CDS             complement(36766..38355)
                     /gene="prfC"
                     /locus_tag="OO544_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HCR6766238.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     SRLKSEYSVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(38446..39123)
                     /gene="yjjG"
                     /locus_tag="OO544_000551"
     CDS             complement(38446..39123)
                     /gene="yjjG"
                     /locus_tag="OO544_000551"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HCR6766239.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(39138..39584)
                     /gene="rimI"
                     /locus_tag="OO544_000552"
     CDS             complement(39138..39584)
                     /gene="rimI"
                     /locus_tag="OO544_000552"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HCR6766240.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRVLLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(39553..39966)
                     /gene="holD"
                     /locus_tag="OO544_000553"
     CDS             complement(39553..39966)
                     /gene="holD"
                     /locus_tag="OO544_000553"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HCR6766241.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            40069..41100
                     /gene="rsmC"
                     /locus_tag="OO544_000554"
     CDS             40069..41100
                     /gene="rsmC"
                     /locus_tag="OO544_000554"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HCR6766242.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSRQMGDNARFSLVATVDDVADCDMLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANCVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
BASE COUNT         9786 a        11332 c        10476 g         9770 t
ORIGIN      
        1 aagccaggcg attactaacg gttttcagcg cagtatgttt tccaatgagg cgggaatggg
       61 ttcgacgcca aacgcggcag cggcagcggc gtcctggcct ccgcatccgg cagcgcaagg
      121 gattgtccag atgattggca tttttatcga caccctggtc atctgtacgg caagcgccat
      181 gctgatatta ctggcgggta acgacacaac ctacatgccg ctggaaggta ttcagcttat
      241 ccagaaggcg atgcgggtgc taatgggttc ctggggtgct gagtttgtta ccctcgtggt
      301 tattctgttt gccttcagct ccatcgttgc caactacatt tatgccgaaa acaatctctt
      361 ctttttacgc ctgaacaacc ctaaagcgat ctggtgtttg cggatctgca ccttcgcaac
      421 ggtcatcggc ggcaccttgc taagtcttcc gctgatgtgg caactggcag atatcataat
      481 ggcctgcatg gctattacca atttgaccgc cattttactg ctctcgcctg tggttcatac
      541 cattgccagt gattatctac gccagcgtaa actcggcgtg cgcccggtgt ttgatccgtt
      601 gcgttatccg gatatcggtc gccagctttc tccggacgcg tgggatgatg tttcgcagga
      661 gtaatcacaa ctatcgatca actcattctc tttttttgct aaagtcggca taaatttcct
      721 gcaaggactg gatatgctga ttcttatttc acctgcgaaa acgcttgatt accaaagccc
      781 gttgaccacc acgcgctata cgctgccgga gctgttagac aattcccagc acttgatcca
      841 tgaagcgcgg aaactgacgc ctccgcagat tagcacgctg atgcgcatca gcgacaaact
      901 ggcgggtatc aacgccgctc gctttcatga ctggcagcca gatttcacgc cggagaatgc
      961 ccgccaggct attttggcgt ttaaaggtga tgtctacacc ggcttgcagg ccgaaacttt
     1021 cagcgaagac gatttcgatt ttgcccaaca gcatttgcga atgctttccg gcttgtatgg
     1081 cgtactgcgc ccgctcgatt taatgcagcc ttatcgtctg gaaatgggga tccgtcttga
     1141 gaatgcccga gggaaagatc tgtatcaatt ctggggagat atcatcacca acaagctgaa
     1201 cgaggcgctc gcagcacaag gcgataatgt ggtgattaat ctggcgtcag atgagtattt
     1261 taaatcggtg aagccgaaga aattgaatgc cgagattatc aagccggtgt tcctcgatga
     1321 gaagaacggc aaatttaaga taatcagctt ctacgctaag aaagcacgcg gtctgatgag
     1381 tcgtttcatt attgaaaatc ggctgaccaa accagagcaa ctgactggtt ttaatagcga
     1441 aggttacttc tttgatgaag cttcctccag caatggcgaa ctggtgttta aacgctacga
     1501 gcagcgttaa gtttgcagtc aatgccggat gcggcgtgaa cgccttatcc ggcctacaaa
     1561 agcatgcaga ttcaatatat tgcagaattc acgtaggcct gataagacgc gccagcgtcg
     1621 catcaggcgc tgaatgccgg attgttaccc ggcatttgtc atttagcggt gatgtttgcc
     1681 tggaccatga ccgccgtgat gatcatgagg agctttctta tggtggcgcg gcggtggcgg
     1741 cggtccgtgt gggtgccagc gattgcctcg ccattcataa tgttgtttcc accagccgtg
     1801 gtcgcgccag tgacctccat cccagtaata gccacgatta tcacgatcgc ctatctgtaa
     1861 ttttactgac gggactaacg taatttccgc agcctgtgct gccatgggag cgaccagaac
     1921 cagggaaagt gcgagtacga tagattgcat ctttttcaca ggttactcct tttatcattg
     1981 cccgttatgg gtcgatatcc ggagattacg cgatgagaac gacggttgtt attctccgca
     2041 atcctaatct ctaagttacc gcatttattg agaatttctc ctttttccct gtcgtttttc
     2101 tccataattt cttcataaaa aagccgggct gcataaaagc aaacccggcc tgattgagat
     2161 aatgaataga ttttactgat gattcatcat caatttacgc aacgcagcaa aatcggcggg
     2221 cagattatgt gaaagcaagg gtaaatcagc acgttctgcc agctcttttg gcagatccaa
     2281 cgtttcaccg agaatcgctt ccacgctctc tttaaatttc gccggatgcg cggtgccgag
     2341 gaacaagcca tattcgcctg gattcaactg gtcacacagc gcacgatacg ctacggcagc
     2401 gtgcggctcc gaggtgtagc ccagttcttt taactcacgc attgtctgtt gcgtggtttc
     2461 atcatccacg gctgcataac ccagctcttt cagttgccag attttgcggc ggaacaactc
     2521 ttccacacgc ggccagttgt tcggctgact cacgtccatc gcgttggata acgtcgcctg
     2581 agtcgctttg ggtgaccact gaccgtcgtg caggaaacgt ggcacggtat cgttcacgtt
     2641 ggtcgcagca ataaaacgtt tcaccggcag accgagtgac ttcgccagca gacccgccgt
     2701 caaatcgccg aagtttccgc ttggcaccga gacaaccagc tggttgcgcg cttcctgcgg
     2761 cagctgcgca actgcttcaa agtagtagca aatctgcgcc agcaaacggc tgatgttaat
     2821 cgagttagcc gagtttaacc ccagcgccac tttcagttct tcatcatcaa acgcctgctt
     2881 caccagcgcc tgacaggcat cgaaatcgcc gtcgatggca acagtttcga tattgccgcc
     2941 caacgtacag aacagttttt cttgcagtgg actgattttg cctcgtggat agaggataac
     3001 cactttcaca ttcggcaaac cgtagaaagc atgagccact gccgctccgg tatcaccgga
     3061 ggtcgcggtc agaatggtca ctggcttatc gcccgcaata tgggtcagca tttgtgccat
     3121 aaagcgaccg ccgaaatctt taaatgccag cgttggcccg tggaacaatt ccagacaacc
     3181 gacatcgctt tcaacattgg cgaccggagc cgggaaggca aacgccgcgc gcacgcgctc
     3241 ttccaggatt tcctgcggga tttcatcacc aataaacgcc gagaggatct tcgcactgcg
     3301 ggtgacaaaa tccagcttca gcatctcatc aatttcagtc aggctgaatt ccggcaggtc
     3361 atgcggaaaa aacagcccct gatttttgcc caacccctgg gttacggctt gcgcaaagct
     3421 gacctgctcg ttgtgatctt tcagattgta gagtttcatt tagttttcca gtactcgtgc
     3481 gcccgccgta tccagccggc aaatatgaac aaaaccttcc tgattttgca ggtagttctt
     3541 acccaaccag tcggcaacgc gctgggcggt atccggcttg tcacatagag cgaacaaggt
     3601 cgggccggag ccggagatac cgctcgctac cgcgccgatt tccgcgaccg cctgccgcgc
     3661 ctgccggaag ccaggcagta accgttcacg gtagggttca gcgataacat ctttcatcag
     3721 cttcgcggca agctcaggct gacgggaata gcaggcgtga atgaagccag ccagatgtcg
     3781 cccgtgcgca atgcaatcct ggcggcgata ctgcgccggt aaaatagccc gggcttctgc
     3841 cgtcgagact ttaattcccg gatacgccag cacccacagc cactcatcaa accctggcac
     3901 ttgctggctg atgatgtcgt tttcttcgat catcaactgc ataccaccaa gaaaacacgg
     3961 tgccacgttg tcgtaatgaa tgctgccgga gatacgtcct tccagctcgc ccatcaaagc
     4021 cagcaaacgg gtgtcattaa gtggcttgcc gcagtgttca ttcatcgcca tcagcgccgc
     4081 gaccaccgaa caggcgctgg agcctaagcc cgagccgatc ggcatattct tttccagagt
     4141 catcgccact ggaatttgct tgcccagctc caggcaaaaa cgctcccagc actgataaac
     4201 gatattttcc cgtggttctg acggcagctt atcggcaaag cgtccgaggt tgttgagact
     4261 gaatgtctct gccgcctcaa ccgtgactac atctccgagc aatgcaccat caacgggtgt
     4321 caccgccgcc ccgagcacat caaacccgac gctcatattg gcactggaag ccggggcata
     4381 aactttaacc atgtcagact cctaacttcc atgagagggt acgtagcaga tcggcaaaga
     4441 caccagcagc ggtaacgtca ttgcccgcac catatccgcg cagcaccaac ggcagcggct
     4501 gataatagtg gctataaaag gccagggcgt tttcgccatt tttcactttg aacagcggat
     4561 cattcccatc cacttcggca atcttcacgc ggcagacacc atcttcatca atattgccaa
     4621 catagcgcaa aacttttcct tcatcacggg ccttcgccac gcgcgcggca aagagatcgt
     4681 cgagctgtga cagattcgcc ataaaagcgg caacatcacc ctcggcgtta aactctgcgg
     4741 gcagcacagg ttcaatttca atatccgcca gttccagttc acgtcccgtt tcacgagcga
     4801 gaatcaatag tttacgcgcc acatccatac cagaaagatc atctcgcggg tccggttcgg
     4861 tataacccat ttcccgcgcc agcgtggtcg cctcggagaa actcatgcct tcgtctaact
     4921 tgccgaagat ataagaaagc gaacctgaaa gaatgccgga gaacttcacc aattcatcac
     4981 ctgcattgag cagattttgc aggttctcaa taaccggtaa tccagcccca acgttggtgt
     5041 catagaggaa tttacgccgc gatttttccg ccgcataacg caactgatga tagtaatcca
     5101 tcgacgaggt gttggccttt ttgttcggcg tgacaacgtg gaaaccttcg cgcaggaagt
     5161 cggcatattg atccgccact gcctggctgg aggtgcagtc aacaatgacc gggttcagca
     5221 gatgatattc tttcacgagg cgaattaagc gcccgagatt aaacggctct ttggcttgtg
     5281 caagttcttc ctgccagttt tccagattaa ggccatgcac actagtgagc agagccttcg
     5341 agttggcaac gccgcagaca cgtaagtcga tatgtttatt cttcagccag ctttgctgac
     5401 gcttcagttg ctccagcagc gcaccgccaa cgccaccgac gccaatcaca aacacttcga
     5461 taacctgatc ggtattgaac agcatctgat gagtaacgcg cacgccagtg gtcgcatcat
     5521 cgttatttac cacgacagag attgagcgtt cagaagatcc ctgagcaatg gcgacaatgt
     5581 tgatattggc gcgggccagc gcggcaaaga atttcgccga gatcccacgc aaggtgcgca
     5641 taccatcacc taccaccgag ataatggcca gccgttccat caccgccagc ggctccagta
     5701 agccttcttt cagttccagg tagaactctt cctgcattgc ccgttcagcc cgcacacagt
     5761 cgctttgcgg aacgcagaaa ctgatactgt attcggaaga tgattgcgta atcagcacca
     5821 cggaaatacg ggcgcgtgac atcgcggcaa agacgcgcgc cgccatgccg accattcctt
     5881 tcatccccgg gccggaaacg ctgaacattg ccatgttatt cagattggaa atgcccttga
     5941 ccggtaattc gccttcatca cgactggcac caatgagcgt acctggtgct tgaggatttc
     6001 cggtattttt aatcaggcaa gggatctgga actgggcgat gggggtaatg gtgcgggggt
     6061 gaagaacttt agcgccgaag taggaaagct ccatcgcttc ctggtaggac atcgacttca
     6121 acaacctcgc atcgggcacc tgacgcgggt cgcaggtata gaccccgtca acgtccgtcc
     6181 aaatctcgca acaatcggcg cgtaaacagg cagccagcac cgccgcggag tagtcggaac
     6241 cgttgcgtcc aagtaccacc agttcgcctt tttcattacc ggcggtgaag cctgccatca
     6301 gcaccatgtg atcagccgga atacgacttg ccgcaatacg gcgggtggac tcagcaatat
     6361 cgacggtaga ttcgaggtaa tgccccactg ccagtagttt ttcgaccgga tcgataacgg
     6421 taacgttgtg accacgcgct tctaacacgc cggccataat ggcgatcgac attttctcgc
     6481 cacggcaaat cagcgcagcg ttaatgctat ccgggcactg ccctaacaaa ctaatgccat
     6541 gcaggacatg ttttatttgg gcaaattcct ggtcgacgaa agttttcaat tgcgccagcg
     6601 ggaatcccgg ctgggcggcg gcgagtcccg tcaaaagttc ggcaaaaata cgttcggcat
     6661 cgctgatatt gggtaaagca tcctggccgc taatggtttt ttcaatcatc gccaccaggt
     6721 ggttggtgat tttggcgggg gcagagagga cggtggccac ctgcccctgc ctggcattgc
     6781 tttccagaat atcggcaacc cgcagaaaac gttctgcatt tgccactgat gtaccgccga
     6841 acttcaacac tcgcatggtt gttacctcgt tacctttggt cgaaaaaaaa agcccgcact
     6901 gtcaggtgcg ggcttttttc tgtgtttcct gtacgcgtca gcccgcaccg ttacctgtgg
     6961 taatggtgat ggtggtggta atggtggtgc taatgcgttt catggatgtt gtgtactctg
     7021 taatttttat ctgtctgtgc gctatgccta tattggttaa agtatttagt gacctaagtc
     7081 aataaaattt taatttactc atggcaggta accagttcag aagctgctat cagacactct
     7141 ttttttaatc cacacagaga catattgccc gttgcagtca gaatgaaaag ctgaaaaata
     7201 cttactaagg cgttttttat ttggtgatat ttttttcaat atcatgcagc aaacggtgca
     7261 acattgccgt gtctcgttgc tctaaaagcc ccaggcgttg ctgtaaccag tcgaccagtt
     7321 ttatgtcatc tgccactgcc agagtcgtca gcaatgccat gactcgttcg cgtaaagctt
     7381 gcagttgatg ttggtctgcc gttgtgtcac ttttcgccgg ttgttgtatt aatgttgcta
     7441 attgatagca atagaccatc accgcctgcc ccagattgag cgaaggataa tccgccacca
     7501 tcggcacacc agtaagaacg tcagccaacg ctaactcttc gttagtcagc ccggaatctt
     7561 cgcgaccaaa caccagcgcg gcatggctca tccatgaaga tttttcctct aacagcggca
     7621 ccagttctac tggcgtggcg tagtaatgat atttcgcccg actgcgcgca gtggtggcga
     7681 cagtgaaatc gacatcgtgt aacgattcag ccagtgttgg gaaaacttta atattatcaa
     7741 taacatcacc agatccatgt gcgacccagc gggtggctgg ctccaggtgt gcctgactat
     7801 cgacaatccg cagttcgcta aaacccatcg ttttcattgc ccgcgccgct gccccaatat
     7861 tttctgctct ggcgggtgcg accaggataa tcgttatacg catattgcca ctcttcttga
     7921 ccaaataacc gcgaacaggg tgatcactgt caacttatta cgcggtgcga atttacaaat
     7981 tcttaacgta agtcgcagaa aaagcccttt acttagctta aaaaaggcta aactatttcc
     8041 tgactgtact aacggtttaa ttgttaaaaa atgctacata tccttctgtt tacttaggat
     8101 aattttataa aaaataaatc tcgacaattg gattcaccac gtttattagt tgtatgatgc
     8161 aactagttgg attattaaaa taatgtgacg aaagctagca tttagatacg atgatttcat
     8221 caaactgtta acgtgctaca attgaacttg atatatgtca acgaagcgta gttttattgg
     8281 gtgtccggcc cctcttagcc tgttatgttg ctgttaaaat ggttaggatg acagccgttt
     8341 ttgacactgt cgggtcctga gggaaagtac ccacgaccaa gctaatgatg ttgttgacgt
     8401 tgatggaaag tgcatcaaga acgcaattac gtactttagt catgttacgc cgatcatgtt
     8461 aatttgcagc atgcatcagg caggtcaggg acttttgtac ttcctgtttc gatttagttg
     8521 gcaatttagg tagcaaacat gcagaccccg cacattctta tcgttgaaga cgagttggta
     8581 acacgcaaca cgttgaaaag tattttcgaa gcggaaggct atgatgtttt cgaagcgaca
     8641 gatggcgcgg aaatgcatca gatcctctct gaatatgaca tcaacctggt gatcatggat
     8701 atcaatctgc cgggtaagaa cggtcttctg ttagcgcgtg aactgcgcga gcaggcgaat
     8761 gttgcgttga tgttcctgac tggccgtgac aacgaagtcg ataaaattct cggcctcgaa
     8821 atcggtgcag atgactacat caccaaaccg ttcaacccgc gtgaactgac gattcgtgca
     8881 cgcaacctgc tgtcccgtac catgaatctg ggtactgtca gcgaagaacg tcgtagcgtt
     8941 gaaagctaca agttcaatgg ttgggaactg gacatcaaca gccgttcgtt gatcggccct
     9001 gatggcgagc agtacaagct gccgcgcagc gagttccgcg ccatgcttca cttctgtgaa
     9061 aacccaggca aaattcagtc ccgtgctgaa ctgctgaaga aaatgaccgg ccgtgagctg
     9121 aaaccacacg accgtactgt agacgtgacg atccgccgta ttcgtaaaca tttcgaatct
     9181 acgccggata cgccggaaat catcgccacc atccacggtg aaggttatcg cttctgtggt
     9241 gatctggaag attaatcggc tttaccaccg tcaaaaaaaa cggcgctttt tagcgccgtt
     9301 tttatttttc aaccttattt ccagatacgt aattcatcgt ccgttgtaac ttctttactg
     9361 gctttcattt tcggcagtga aaacgcatac cagtcgatat tacgagtcac aaacatcatg
     9421 ccggccagcg ccaccaccag cacactggtt cccaacaaca gcgcgctatc gacagagttg
     9481 agcagtcccc acatcactcc atccagcaac aacagcgcga gggtaaacaa catgctattg
     9541 cgccagcctt tcaataccgc ctgcaaataa ataccgttca ttaacgcccc aatcagactg
     9601 gcgattatcc atgccacggt aaaaccgata tgttcagaaa gcgccagcaa gagcaaataa
     9661 aacatcacca atgaaagccc caccagcaaa tattgcattg ggtgtaaacg ttgcgcggtg
     9721 agcgtttcaa aaacaaagaa cgccataaaa gtcagtgcaa tcagcagaat ggcgtactta
     9781 gtcgcccggt ccgttaattg gtattgatcg gctggcgtcg tcactgcgac gctaaacgcg
     9841 aggaagtttt cccagccggt atcattgcct gaagcaaaac gctcaccgag attattagca
     9901 aaccagctgc tttgccattg cgcctgaaaa cctgactcgc taacttcccg tttggctggc
     9961 aaaaaatcac ctaaaaaact gggatgcggc cagttgctgg ttaaggtcat ttcgctatta
    10021 cgcccggcag gcaccacaga aagatcgccg gtaccgctta aattcagggc catattcagc
    10081 ttcaggttct gcttccgcca gtccccttcg ggtaaaggga tatgcacgcc ctgcccgcct
    10141 tgctctaacc cggtgccggg ttcaatggtc agcgccgttc cgttaacttc aggcgctttc
    10201 accacaccaa taccacgcgc atccccgacg ctaatcacaa taaatggctt gccaaaggtg
    10261 atatttggcg cgtcgagttc gctaagacgc gaaacatcga aatcggcttt tagcgttaaa
    10321 tcactgtgcc agacctgacc ggtataaatc cctatcttgc gttcttccac gttctgattg
    10381 ccatcaacca tcaatgactc aggtaaccaa aaatggataa aacttcgttt ccgctccacg
    10441 gttttatcct cttcctgtac cgtataaagc tcggtcacag gaatagcgat aagcggccca
    10501 acaagttttt gcggcccgct ggtactttgg cgaatcgcat cttccacatc gctacggtaa
    10561 tcggcacgtt cgacaatcac ctgccgaatc agcattatcg gaatcaacaa cagcaatact
    10621 gcaccaaaca gggtggtcat tttccagaac agggaggatt tcaacatagt cttctccttt
    10681 gcaatggcag caggatacga aggctatgtg ggaagaattt gaagctatgt gaagtgacgg
    10741 tgaagtcgaa gcgaggccag cacgccacct tcctgcacgt tgcgcagcgt gacttcgccg
    10801 ttaaacaaac gggcgacctc actgacgaac gccaacccca gaccgctgct tttttgccca
    10861 tttgcacgcg gcaaagagta aaagcgttca aaaatacgtg acagcgcgta gtcaggaata
    10921 ccactaccgg tatccagcac cttaagcgtg acgtgttcct gatccacttc ggcgcttagc
    10981 gttatacgac cgctctcggg ggtaaaatcg atggcgttat ccagtaaatt ccccagcgcc
    11041 tgatccagta acgccggttc agcagcaaca ttaacctcgg tgggcgtaac atgtagagtg
    11101 atgttttttt ctgccaactg cacggtgcgc gcttcgctga cgcggcggaa taatgccgcc
    11161 acatcaacag cagtcaaaac gacttcctga cgattctcca gtcttgcctg gcgtagtaac
    11221 gtttccacca gtgcctgcat tcgcgcattt tgcgtcagaa tgttgtcagt aaaacgagcc
    11281 actacttcag gcggcggacc ttcgcgtaaa atttccgccg cgccacgaat cgccgccagt
    11341 gggcttttta gctcatgggt taacgcataa acatactgct caatatagtt tttcccttcc
    11401 agcttcacgc gcatactttc cagcgcctgc gcgagtttac gcagctcgct actaccgaga
    11461 tcgggaagag gaacgggctt attgtcagtg acggaatcag catagcgagt gagccgggca
    11521 atggagcggt tgatccacca aaccatgcct gcgccaatca ccagtgcgat ccccaacaaa
    11581 atggcgctgg cccataaaat tcgccgctcg ctgcgtttaa tgaccggagc catcgccgcg
    11641 ttcggtttgc ctacgctcaa aacgccaata agccgcgagc cgtccataat cggcgcggca
    11701 acatacatca ccgaactttc gggatcggca ggattttgca acgtgctacg cgcaccatac
    11761 tgaccacgca acgttagcca gacgtcattc cagcgcgaat aatcctgccc aacggcttta
    11821 tttgccgaat cgaacaatac tttgccctgc gcatcggtca tatagacatg atattcattg
    11881 cgcactttgt taatgccacc gatattggcg cgaaacgggc gatgttgtag ctgattaaac
    11941 gcctgagcca gttgcccatg cgttgggtcc ccagagagca aatcgggacg cgccagctcc
    12001 gccagcaacg ttgcggtatc gattaacgtc ccctccgttg ctcttcgcac gcccggttta
    12061 acttctttga caaaaatagc cagtacgaac caggccgcca ccgccaccag taaaaaatag
    12121 cccagcagca accgcatgcc gatacgcatt acaggcccct caggctatat cccatgccgc
    12181 gatgagtatt aatcggagaa agatcggggt tgatggcgcg cagcttggca cgcaacgttt
    12241 taatgtgggt atcgacggtg cgatcgtagg tgtcctgcgc atcttcccac acgctatcca
    12301 ttagttgctg gcgggaccag acgcggcctg gcgacttgag taacgtcttc agcaataaaa
    12361 actcataccg cgtcagcgtt aatggcgtgt caaaccagct gatctgcgcc gcgggttcat
    12421 tcaattcaaa atgaccaata cggatgacgg gagacggcga cgagaacttc ttcacccgac
    12481 gcagtaaggt gcgcaccctg gcgcacactt cgcggggtga aaacggttta gccacatagt
    12541 cgtcagcgcc aatttccagc ccaagcaggc gatcgacctc ttcacttcgg gccgtcagga
    12601 acagtacagg taacgccgga tggagcgcca gtaactggcg gcacaattca aagccgctga
    12661 tatcaggcaa accaacatcg agaatcatga cgtcgggaac ctgctggcga gctttatcca
    12721 gcaccggcaa gcctcgctca aagacctcga cggcaaaacc ttcctgctgc aacatgtaga
    12781 ccagcgtgtc ggctatccct tgctcatctt ccactaacca gaccgtttcc cgttgcataa
    12841 atcgcctctg ttattgccgc cacggcatga caggaaccgc gctaatcgcg tttttcggcg
    12901 aaccttctac aactttgtcg gagtaagcca gataagcgag cgcgttgcgt ttggcgtcat
    12961 aaaagcgcac gacctgtaac gacttaaaga ccagggacgt gcgttttttg aatactacct
    13021 cgccctgagc tttgccgttt ttaatacgat ccgacagttc aatcggcccg acttgctgac
    13081 aagaaatagc cgcatcggag gtatcttccg ccagacccaa tcccccttta ataccacctg
    13141 ttttcgcccg gctcacataa caggtgacat ttttcacatc gggatcgtca aaggcttcca
    13201 caacaatttt gtgatccggg ccgatcattt taaatacggt gtcgaccgaa ccaatctctt
    13261 ctgcatgagc caatggcccc agcattatca ggcttaaaga caggatcaaa tgcttgtatt
    13321 tcatattgtt accattttta tctattacac gagcaattag ttcgtcacgg taaataacat
    13381 attgattaag aacaaaatct caatactttt atttccgtca agccctaaaa catactctac
    13441 taagaaaaaa acactgaatg ctaaaacagc aaaaaatgct attatccaat tacctgatgt
    13501 caggtgctcg ttgttgaaag gatgaggata ttttatggat caggccggca ttattcgcga
    13561 ccttttaatc tggctggaag gtcatctgga tcagcccctg tcgctcgaca atgtagcggc
    13621 gaaagcaggt tattccaagt ggcacttaca gagaatgttt aaagatgtca ctggccatgc
    13681 tattggcgcg tatattcgtg ctcgtcgttt gtcgaaatcg gcggtcgcac tacgcctgac
    13741 tgcgcgtccg attctggaca tcgcgctgca ataccgcttc gactctcaac agacatttac
    13801 ccgcgcattc aagaagcagt ttgcccagac tcctgcactt taccgccgtt ctcctgaatg
    13861 gagcgccttt ggtattcgcc cgccgctacg tctgggtgaa ttcacaatgc cagagcacaa
    13921 atttgtcacc ctggaagata cgccgctgat tggtgttacc cagagctact cctgttcgct
    13981 ggagcaaatc tctgatttcc gccatgaaat gcgttatcag ttctggcacg attttctcgg
    14041 caacgcgccg accattccgc cagtgctcta cggcctgaat gaaacgcgtc cgagtcagga
    14101 taaagacgac gaacaagagg tattctatac caccgcgtta gcccaggatc aggcagatgg
    14161 ctatgtactg acggggcatc cggtgatgct gcagggcggc gaatatgtga tgtttaccta
    14221 tgaaggtctg ggaaccggcg tgcaggagtt tatcctgacg gtatacggaa cgtgcatgcc
    14281 aatgctcaac ctgacgcgcc gtaaaggtca ggatattgag cgatactacc cggcagaaga
    14341 cgccaaagcg ggagatcgcc caattaatct gcgctgtgaa ctgctgattc ctatccgccg
    14401 ttaacgctgc agctcatcta atgcaggggc gtccagatgc gagatgtccc ctgcggtttc
    14461 aacgacccag ccagacgcca gccacaggct ttcctgataa tccacgcgcg aaatagaaca
    14521 gttacgcaga cgtaagcggc gctctgccca tgctggtaat ccgagaatcg tactcaccag
    14581 acatcccagt gcaataccat ggctcaccag cagcggtcgg cttccctgcg gtaagtcccg
    14641 gcaagattcc agtgccgcat tgacgcgatc gctgagctcc tgcattgact cgccttcagg
    14701 aatacgcccg tcaacggtgc cattgaccag ctgccgacgc cagttctctt cttcttcggt
    14761 cagagaatcg atatgtcttt tctccagcac acccatgttt aattcacgca ggcgagaatc
    14821 aaagatgatg tcacagccgc aggcctgggc gatgatttcc gccgtacgcc gggtgcgtcc
    14881 taaatcgcta ctgataatat gcgtaatgcc aagctctttg gcacgggttg ccacctgcat
    14941 cgcctgttgc tcacctttgg cggtcagcgg gctgtcagac tggccctgaa tacgtcgctc
    15001 ggcgttccac tgcgtttcac cgtggcggac taggtatacc tgtaacatgc tttttttccg
    15061 tatactgctg taacgttaat tttctgagta atgctgatta tgcaccaagt tatctgtgcg
    15121 accaccaatc ccgctaaaat tcaggccatt ctgcaggcat ttcacgagat cttcggcgaa
    15181 ggatcctgcc atattgcatc cgttgccgtc gagagcggtg taccggaaca gccctttggc
    15241 agtgaggaaa cgcgcgctgg cgcacgaaat cgggtagcaa atgcccgccg tttacttcca
    15301 gaggctgatt tttgggtggc gattgaagct ggcatcgatg gtgacagcac tttcagttgg
    15361 gtggtgattg aaaacaccag ccagcgcggc gaggcgcgtt cggctacttt gccgttacct
    15421 gcggtgattc tgcagaaagt gcgtgaaggt gaggcgcttg gtcccgtgat gtcgcgttat
    15481 accggtattg atgagattgg tcgtaaggaa ggggcgatcg gcgtgtttac cgccgggaaa
    15541 ctcactcgcg ccagcgtcta tcaccaggcg gtgatcctgg cgctgagtcc gtttcacaat
    15601 gccgtgtatc aggcattgta ggacggataa ggcgttcacg ccgcatccgg cattcggtgc
    15661 acgatgcctg atgcgacgct gccgcgtctt atcaggccta caaaatcaat cgcttttcag
    15721 caacacctct tccagccact ggcgcagctc cacgggcgcg gctttcaggc tgttagatcc
    15781 acgcgtaatc gtcgcgatgc ccgcgccgag ttcatttttt aactcacgct gactcatttc
    15841 gccacgcaac agctcttcga caatacgcac gcgagtcccc aacgcttcgc gctcatctgg
    15901 cgtcagcatc aggtttaaca acggtaaatg gagatcgttt tggtaggcat tcttaagcag
    15961 gtcgacaaaa cgtaaccact cctggtgacg ctgttctgcc atcgctgctg aatagggtga
    16021 ttgttgggcc ataatatgtc gccattgtta gcgggggaag caaaatgcct cccccggttg
    16081 tactcgctaa agagtacgat agcatatcat aaacgtgcgg atcagtaacg acgtccccat
    16141 tccgtggcgc tcatcaacgt cggtttatcc cccatgaaat aacggtagta agcgtcataa
    16201 gccagcacgt tcttcacata accgcgcgtc tcggagaacg gaatactctc gacaaatgcc
    16261 acagcgtcga tacgcccggc gctgttgcca agccaggttc gcacccgtcc aggtccggcg
    16321 ttataagctg ctgaggagaa aatacggtta ttgccaaact gctgataaac atattgcagg
    16381 taactggtgc caatgttgat attcgtttcc ggatccagca attgcccagg gctgctataa
    16441 ccaggaatag agaacatctt caccgtatgg gtcgctgtac caggcataat ctgcatcagg
    16501 ccgctggccc ctaccggtga tttcactttc ggattccagg cgctctcctg acgggcaatc
    16561 gccatcgcat agctttgcgg gatctctttc ccactggtgt aacgtttgaa aagatcgttg
    16621 taagccagcg ggaatcgctc ttccagatga tcccaaagct tcccggcgat cgttgcctga
    16681 acgctaagat cccaccattg gttattgaaa gcataccgag ccagttgagc ctgctctgtt
    16741 tttgacttgc tcttcaccag attggcccac tcgctacgcg cggtgttatc gagattccag
    16801 tacatcaact cgcgcacgcg cgccatctcc ggcccctgag tcagggcgct gtcaacattc
    16861 tgcggcgctt tatcaatctt cagctcatac tcttctccga tgcgttgtgc tgcaaccatc
    16921 gggtagaaac cacgctgttg catgagttga tgcaaaatct ctttcgcttc agcttcacgt
    16981 ccgcgttcca gcaataaatc cacctgccag taacgccatt catctttctc ttttgcttcc
    17041 atcggcagac gcgccagcca ggtattcagg ccgcgacgat cgccggtgcc aagtgccatt
    17101 cgcacacggc gttcaatcaa cgacgtggat tgcgagcgca taatgacgtc atcgcgccat
    17161 ttcgcctgct cgtcggtgac atcgttgccc atcaaacgcc aggcgacgat atcgcgcagc
    17221 tcctgaatct gatcttcatt aagctgctgc acctgggcaa gcgacgggat catcagccgt
    17281 gcattttcag catcctgccg cgccacactg gcaaacgcca ccgctgccat ttgacgggta
    17341 aaatcggtcg ctccggttgt acgcgcgaag gtcagtaccg tattagggtt gttcgccagt
    17401 gaaatgattg ccgaggcgat agtctggtaa tcggcaggca tctgccctgc cagcactgtt
    17461 accaggccag tgttacccgc tttcatcgcc agacggatac gctctaaata cgccagcgga
    17521 tcttgtttac ctgacgcacg ccagacgcta aataacttgt cacaggcgtt aggctggctc
    17581 ttgccggtta gccacagctc tttcgcccct tgccaggctt cttcactctg cccggtgttc
    17641 catttcgcat agtagtaatt acattgcgct tcggtagttc cgggcttttc cgggctaaag
    17701 gctaacaagc cacgccagtc ttcacgccgc gccagttcat tgacgaaacg agattgcagc
    17761 gtgcgagcgg gaggaagcgt ggggttagcg cgaacaaagt tagtgaccgt caccgccggt
    17821 tgattcatca gatcatcggt gatctggcgg tattccaggt agggataaag cggataatcc
    17881 ttcagtccag gcatcatttg ttcgaccaca tccatttgtc gattatccca ggcctgcttg
    17941 atttgcgcgt aacggctacg ctgctcatcc agtgagtcgg ctcgcgccac gctgctgacc
    18001 gtcagcagac agacaccggc agccaacagc cgccaggtaa cttgtttggc tttttccaca
    18061 agcgcatcct ctaagtgtaa atacatcaat gcagcgtcat gccgcgtaat agatttatgc
    18121 taaccagtca ttgccgttta cgccacgtta cggacacttt tttactttta ctgcgaaggt
    18181 gatcgaccaa ctgggattag gcggggaaaa aggctacact tcgcctttga aaactcatca
    18241 tcacgataaa catagaggcg aagtccaacg tggctcaatt cgtttatacc atgcatcgtg
    18301 tcggcaaagt tgttccgccg aaacgtcata ttttgaaaaa catctctctg agtttcttcc
    18361 ctggggcaaa aattggtgtc ctgggtctga acggcgcggg taagtctacc ctgctgcgca
    18421 ttatggcggg cattgataaa gacatcgaag gtgaagcgcg tccgcagcca gacatcaaga
    18481 ttggttacct gccgcaggaa ccgcagctga acccggaaca caccgtgcgt gagtccattg
    18541 aagaagcggt ttctgaagtg gttaacgccc tgaaacgcct ggatgaagtg tatgcgctgt
    18601 acgccgatcc ggatgccgat tttgacaagc tggccgctga acaaggccgt ctggaagaga
    18661 tcattcaggc tcacgacggt cataacctga acgtacagct ggagcgtgcg gcggatgcgc
    18721 tacgtctgcc ggactgggac gcgaaaatcg ctaacctctc cggtggtgag cgtcgtcgcg
    18781 tagcgttgtg ccgcctgctg ctggaaaaac cagacatgct gctgctcgac gaaccgacca
    18841 accacctgga tgccgaatcc gtggcctggc tggaacgctt cctgcacgac ttcgagggca
    18901 ccgtggtggc gattacccac gaccgttact tcctcgataa cgttgcaggc tggatcctcg
    18961 aacttgaccg cggtgaaggt attccgtggg aaggcaacta ctcctcctgg ctggagcaga
    19021 aagatcagcg cctggcgcag gaagcttcac aagaagcggc gcgtcgtaag tcgatcgaga
    19081 aagagctgga gtgggtacgt cagggaacta aaggccgcca gtcgaaaggt aaagcacgtc
    19141 tggcacgctt tgaagagctg aacagcaccg aatatcagaa acgtaacgaa accaacgaac
    19201 tgtttattcc acctggaccg cgtctgggcg ataaagtgct ggaagtcagc aacctgcgta
    19261 aatcctacgg tgatcgcctg ctgattgatg acctgagctt ctcgatcccg aaaggggcaa
    19321 tcgtcgggat catcggtccg aacggcgcgg gtaaatcgac cctgttccgt atgatctctg
    19381 gtcaggaaca gccggacagc ggcaccatca ctttaggtga aacggtgaaa ctggcgtcgg
    19441 ttgatcagtt ccgtgactca atggataaca gcaaaaccgt ttgggaagaa gtttccggcg
    19501 ggctggatat tatgaagatc ggcaacaccg agatgccaag ccgcgcctac gttggccgct
    19561 ttaactttaa aggggttgat cagggtaaac gcgttggtga actttccggt ggtgagcgcg
    19621 gtcgtctgca tctggcgaag ctgctgcagg ttggcggcaa catgctgctg ctcgacgaac
    19681 caaccaacga cctggatatc gaaaccctgc gcgcgctgga aaacgccctg ctggagttcc
    19741 cgggctgtgc gatggttatc tcgcacgacc gttggttcct cgaccgtatc gccacgcaca
    19801 tcctggacta ccaggatgaa ggtaaagttg agttcttcga aggtaacttt actgagtacg
    19861 aagagtacaa gaaacgcacg ctgggcgcag acgcactgga gccgaagcgt atcaagtaca
    19921 agcgtattgc gaagtaatgc gtaaaatgcc ggatgcggcg tgaacgcctt atccggccta
    19981 caaaccaaga aatttcaata cgttgtgaat ctccaggcct gataagacgc ggtaagcgtc
    20041 gcatcaggca ttgagcaccg aatgccggat acggcgtgaa cgccttttcc ggcctacaac
    20101 agccttagta tccgcaattt cgtagcgggt cacccgtgca ggttttttca aaccttgttt
    20161 aatctcgaca gcctcttcca gagaggtttt caactcatca aaaaacttat tttatacctc
    20221 gcctttgagc cgtttcatcg cggttatctc tgctccccca tcatctcccg caccagctcc
    20281 acgcagcgca ggaaacggct gtcataatcg tcctcttcaa cccgcacgaa ttcgatattg
    20341 ttctcttcca gcatctccac cagcaagttc tggaactctt tgcgatccac cgaactgccg
    20401 aggctgcgta aaccatccgc cacccacggc gtgttgttct ccagcaggat caccagatcg
    20461 aaacggtatt catcaatcaa cgcctgtacg aacggatgct cacgcccttc gtactttttg
    20521 cagaacgcct gggtagtgac aaaatcagtg tcaataaacg ccactttatt ggcatatttc
    20581 actgcaaaat caatgtattg tgcgtggccc agcgcgattt tatcgtaatc ggaatactgc
    20641 aatgcgatct catcaccgcc gaggtgtgaa aagacataat cgcgaccata ttcccacgca
    20701 ctggtggtat tgaagatatt ggcaagtttg tttaccaggg tggatttacc gctcgactcg
    20761 ccaccaagga tcgccacggt acgtacaaag aacggcttca cttccgtagg aatatattcc
    20821 cagtagcgga acgggttttc gcggatctgc gcaccgctga tactcataaa ggtacgtttc
    20881 ggatcgacca gcaccgtatc gatccccaga tgttccatat actgtggcgc atcggcttct
    20941 tccgaggtgt agatcaggtc cggctgaatc cccttttcag ccataaactt tttgatgccg
    21001 ttgctccaca catcccagcc gtgcggatac ggctccatgc cctcttcgtt gaaagcatga
    21061 atgcgaatat ttttctgata tttaaaagtt tgtaataacc agcgcagacg atccggcacc
    21121 gtgggctgct gcgacatggc gctgtcttca aacaacgcgc ggtcgcgggt atcgtcaaaa
    21181 cccataatga tatgcagctc gtcaacctgg ctacaggcgc gctggataag gtagatatgt
    21241 ccggtatgca gcgggtagaa cttaccgaat acgacaccaa tggttttctt ctgtcgggga
    21301 aattcaagcc ccaaaaaacg gtgcaacgcc tccagctttt gcgcgctggg gcttttaatt
    21361 ttggcattca gtaactggct taaataccct ttggtcatac cgctggcatc agctacctgc
    21421 tgtagcgtgc agccctgttg cttgatggca gttttcaggt aatcaaatga cgacatatct
    21481 ccctccgtat ctctcattat aagtcgtcga acacgctaag cgcgtcggag agttttttaa
    21541 cgccaaaaat ctgcatccct tccggcgctt ttttcggtac gttagccgcc ggaacaatcg
    21601 cccggcgaaa accgtgtttc gccgcttctg aaattcgttc ctgaccgctg ggcaccgggc
    21661 ggatctcccc tgccagcccg acttcaccaa acaccaccag atcctgtggc agcggtctgt
    21721 cacgcaggct ggaaaccatc gccagcagta acgctaaatc ggcactggtt tcggttacct
    21781 tcacgccgcc gaccacgttc acaaatacat cctgatcggc catttgcaga ccaccgtgac
    21841 ggtgcaagac agccagcagg attgccagac ggttttgttc cagccccact gccacgcggc
    21901 gcggattcgc catcatcgag tgatcgacca gcgcctgaat ctccaccagc aacggacgcg
    21961 ttccttccca caccaccatc acggagctgc cggaggtcac ttcatctccg cgacttaaga
    22021 aaattgccga agggttgctg acttcacgca gcccctgttc ggtcatcgcg aagacgccca
    22081 gctcattcac cgcgccgaag cggtttttat ggctgcgcaa ggtgcgaaaa cgggagtcgg
    22141 catcaccatc cagaagcacc gaacagtcga tacagtgttc cagcacttta gggccagcca
    22201 gcgagccatc tttggttacg tgcccaacca tgacaatcgc cacaccgcgc gttttggcga
    22261 agcgcgtcag ataagccgcc gtttcacgca cctgcgccac gctaccaggc gatgattgta
    22321 catccgccat atgcatcacc tggatcgagt caattaccat cagtttcggt tgctcttctt
    22381 cggcaatcag gcagatctgt tcgatgctgg tttccgacaa catattgaga ttgtcagtcg
    22441 gcaggccaag gcgatgagcg cgcattgcca cctgttgcag cgactcttcg ccagtgacat
    22501 acagcgtttt catctgctgg gccagtttgc acagcgtttg cagtagcagc gtggatttcc
    22561 ccgcacctgg gttaccgcca atcagaatgg cgcttcccgg caccacgccg ccgcctagta
    22621 cgcggtcgaa ctctttaaat ccggtggaaa aacgcggcag ctcttcaagg ctgatatcgg
    22681 agagtttctg gacttttgcc accccggcgc taccggcata gccgctgaga cgctcgttac
    22741 gcgccaccgt tggcgacgca gcaagacgca cctcggtgat ggtgttccag gcatgacagg
    22801 cactgcactg cccctgccag cgcggataat cggccccgca ttcattacaa acaaaggcgc
    22861 gttttggagc ttttgccacg gtgtacctcg ttaatgctgt gccgcccgca ggatggcggg
    22921 cgagcaatta cttctgattc aggctgccag agaggatgca gaataccccc atcaggtcag
    22981 cgtgacggat ggtgacttcc gccttttcat tcacttttgg cttggcatgg taggcaatcc
    23041 ccagccctgc tgctttgatc atcggcaggt cattagctcc atcgccaatc gccacggtct
    23101 gcgccagcgg gatttcatac tcctgcgcga ggcgagtcaa agttttcgct ttgtactgcg
    23161 cgtctacgat gtcgccaatc acattgccgg taaatttacc gtccatgatc tccagttcat
    23221 tggctaccac ggcggtcagg cgcagcttgt cgcgcagata ttcagcaaag aaagtaaagc
    23281 cgccggaggc aatcgccact ttccagccca gcgtttccag cttgagcacc agttgcgtta
    23341 agcctggcat cagcggcaga ttttcacgca cctgttgcag aatattggcg tcagcgcctt
    23401 tcagcgtcgc cacacggctg cgcaggctgg cggtaaaatc gagttcgccg cgcatcgccc
    23461 gttcggttac ttccgccacc atctcgcccg ttccggccag tttggcaatt tcatcaatac
    23521 attcaatctg gatggcggtg gaatccatgt ccatcaccag caaacccggc gtgcgcaggt
    23581 gcgggatttt ccccagcggg gcgacatcca gttgcgcttc atgggccagg cgcgtagccc
    23641 gtgcggtgag tgaacctgcc agacgaatca cctgataatc ttccacgcac caggcggcaa
    23701 caatcaccat cgccgcaccc agtttgctct ggtattgggt cagacgctgt ttatccagcc
    23761 cacgaccata cagcagccag ccgctacgac ctgcgtggta atccagtggc atcacttcat
    23821 caccacttaa tgaaagaggc agacccggcc ataaagagac atcttcaggc aggtcgcacc
    23881 aggtaatgtt aggcattaag gctcctgtaa aatcgttcga agcagggaaa ataacgcatg
    23941 aggctacctt gtatccattg cttctggcaa cattaagtct caaattttca aagggtggaa
    24001 gatggctcgc acaaaactga aattccggct gcatcgggca gtgattgtcc tgttctgtct
    24061 tgccttgtta gtggcactga tgcagggggc gtcatggttt agtcaaaacc accagcgaca
    24121 gcgtaatcca cagctggaag aactggcccg caccctggcg cgtcaggtga cgctgaacgt
    24181 tgcaccgctg atgcgtaccg actcaccaga tgaaaaacgc attcaggcga tcctcgatca
    24241 gttaacggat gaaagccgca tcctcgacgc gggtgtgtat gacgaacaag gcgatcttat
    24301 cgcccgttca ggcgaaagcg tcgaagtgcg cgaccggctg gcgctcgacg gtaaaaaagc
    24361 aggcggctat tttaaccagc agattgtcga gccaattgcg ggtaaaaacg gaccgctcgg
    24421 ctatctgcgc ctgacactcg acacccatac gctcgccacc gaagcccaac aggtggataa
    24481 caccactaac attttacgcc tgatgttgct gctctcactg gcaatcggcg tagtgctgac
    24541 ccgcacgctg ctacagggta aacgcacccg ctggcagcaa tcgcccttcc tgttaaccgc
    24601 cagcaaaccg gtgccggaag aggaagaaag cgagaaaaaa gagtgaccca ttactacaag
    24661 aaaggaaatc gttatgtcca cattacgcct gctcatctct gactcttacg acccgtggtt
    24721 taacctggcg gtggaagagt gtatttttcg ccaaatgccc gccacgcagc gcgttctgtt
    24781 tctctggcgc aatgccgaca cggtagtgat tggtcgcgcg cagaacccgt ggaaagagtg
    24841 taatacccgg cggatggaag aagataacgt ccgcctggcg cggcgcagta gcggtggcgg
    24901 cgcggtgttc cacgatctcg gcaatacctg ctttaccttt atggctggca agccggagta
    24961 cgataaaacc atctctacgt cgattgtgct caatgcgctg aacgcgctcg gcgtcagcgc
    25021 cgaagcgtcc ggacgtaacg atctggtggt gaaaacagtc gaaggcgacc gcaaagtctc
    25081 aggctcggcc tatcgcgaaa ccaaagatcg tggcttccac cacggcactt tgctgctcaa
    25141 tgccgacctt agccgcctgg caaactatct caatccggat aaaaagaaac tggcggcgaa
    25201 aggcattacc tcagtgcgtt cccgcgtgac caacctcacc gagctgctgc cggggatcac
    25261 ccatgagcag gtttgcgagg ccataaccaa ggcctttttc gcccattatg gcgagcgtgt
    25321 agaagcggaa atcatctccc cggacaaaac gccagacttg ccaaacttcg ccgaaacctt
    25381 tgcccgtcag agtagctggg aatggaactt cggtcaggct ccggcattct cgcatctgct
    25441 ggatgaacgc tttagctggg gcggcgtgga actgcatttc gacgttgaaa aaggccatat
    25501 cacccgcgcc caggtgttta ccgacagcct caaccccgcg ccgctggaag ccctcgccgg
    25561 gcgactgcaa ggctgcctgt accgcgcgga tatgctgcaa caagagtgcg aagcgctgtt
    25621 ggttgacttc ccggaccagg aaaaagagct acgggagttg tcgacgtgga tagcgggggc
    25681 ggtaaggtaa ctcgctgatt aaaataccaa aggaagggaa tatgatcgat ctggaaaacc
    25741 aggagcggga gattatcaat ttaatgttaa gccagcgtat ttcctggcta gccgccgtgc
    25801 gtatcaggca taaattatca cttgctgaag taagtaaaat gttaggtatc tcgattaatt
    25861 ctctaaaata gatcgaaaag acggaacggc ttagctcaaa catcaaaagc aaaatgggag
    25921 aaatttatgg ttgtccacca gagttgctga tatgcccttc ctggatgacg gctgaacata
    25981 aatgaataga ttcgtgaata gatacttgaa tgtattttat tttttatcca tatgaaaaat
    26041 aatagtaaat ctataaatca gttgctattg atgaatagat actaaaaagc cggagcagtc
    26101 ccccactccg gcttaacacg acaatcgcct ttcagcgaaa cacaattact ctttatcgcc
    26161 cagcagaacg gattccagtg cgattttgat catgtcgttg aaggtggtct gacgctcagc
    26221 ggcagtggtc tgctcgtgag tgcggatgtg gtcagacaca gtgcagatgg tcagggcttt
    26281 cgcgccaaat tctgcagcga cgccgtagat accagccgct tccatttcca cgccgaggat
    26341 gccgtatttt tccatcacgt cgaacatttc gccgtccgga gagtagaaca ggtcagcgga
    26401 gaacaggtta ccaacgcgag catcaatgcc cagtgcttta gccgcgtcta ccgcgttacg
    26461 caccatgtca aagtcagcga tagcggcaaa gtcatgatct ttaaaacgga tgcggttaac
    26521 tttggaatcg gtgcaggcac ccataccgat aacgacgtcg cgcagtttta cgtgcggcag
    26581 aactgcgcca caggaaccca cgcggataat tttcttcacg ccgaaatcgg tgatcagttc
    26641 tttggtgtag atggagcagg acgggatacc cataccgtga cccattacgg aaattttgcg
    26701 gcctttgtaa gtaccggtga agcccagcat accgcgaacg ttgttcactt cacgggcatc
    26761 ttcaaggaaa gtttcagcaa tatacttcgc acgcagcggg tcgcctggca tcaaaactac
    26821 gtcagcgaaa tcgcccattt ctgcattaat gtgtggggta gccattgttt tatccttatc
    26881 aacatgtttt caccctctcc ctgagggaga gggccggggt gagggcatca agccgcaccc
    26941 attatcagaa catggctttg ccatattcca tatcagaagt accaaaatat tttgccagag
    27001 tctggccgat atccgcgaag gtttcacggt gacccagtga gcccggtttt actttcgggc
    27061 cgtaaaccag taccggaatg tgttcacgcg tgtggtcagt accggtccag gtcggatcac
    27121 aaccgtggtc agcagtgagg atcaggatgt cgtcatcgcg cagcagagac atcagctccg
    27181 gcagacggcg gtcgaacagc tccagaccca cagcataacc ggcgacgtcg cgacggtggc
    27241 cccaggaaga gtcgaagtca acgaagttgg tgaagacgat ggtgttatca cccgcttctt
    27301 tcatctcttt gatggtggcg tcaaacagcg cgtccaggcc agttgctttc actttcttgg
    27361 tgataccgca gttggcgtag atgtctgcaa ttttaccgac agaaaccacc tgaccgtgtt
    27421 tttcatcaac cagtttctgc agcacggtcg gtgctggcgg ctcaacagcc aggtcgtgac
    27481 ggttaccggt acgctggaag ttaccggctt tgtcaccgat aaacggacga gcgataacac
    27541 gaccgatgtt gtagccgccg ttggtcagct cttcacgggc gatttcgcac agttcgtaga
    27601 gtttatccag gccaaaggtt tcttcgtggc aggcaatctg gaacacagag tcagcggagg
    27661 tatagaaaat cggcttgccg gttttcatgt gctcttcgcc cagctgatcc agaatgaccg
    27721 taccggaaga gtggcagtta ccgaggtaac ccggcagatt agcgcgttca accagtttat
    27781 ccagcagctc ttgcgggaag ctgttttcgt gatcggagaa atatccccac tcaaatagaa
    27841 ccgggacacc ggctatttcc cagtgaccag acggggtatc tttaccggat gacatttcgt
    27901 gcgcccatgc gtacgcgccg ataacttcag cgttgccgtc cattcccgcc ggaatgaaac
    27961 cggtagaacc ttcgtgtgct ttcgccagcc ccagacgggt cagatttggc agattgagcg
    28021 gacctttacg accgttatca gcttcgcctt tggcacaagc ttctgcgata tgacccaggg
    28081 tgtcagcccc gacgtcacca aagcgttctg catcttctgt agcgccgatg ccgaatgagt
    28141 ccagcaccat aataaatgca cgtttcatat gttctccgta ctcagtgctt caaatttaaa
    28201 tgatcagatc agtataccgt tattcgctga tacggcgata gacagttggt gtgctttccg
    28261 gtgctttatc ggcaagttta attgccgctt tcaccgcttt cgccgcttcc tgccagctgt
    28321 tttcgtcttt cgcgtggata accgccagcg gacgctgacc gtctacctgg tcgcccaaac
    28381 gcgccatatc agtaaagccg acgctgtaat caatggtgtc agatgcctga cgacgaccgc
    28441 cgcccattgc aaccactgcc atccccagcg cgcgggtatc catttcactg acaaaacctt
    28501 cggtatcagc atagactgct ttcgtcagca tcgctgtcgg cagatacttc gcgtagttct
    28561 caacgaagtc ggttgggcct ttttgtgccg ctaccatacg accaaagact tctgccgctt
    28621 taccgttgtc cagcactgct tgcaatttcg cgcgcgcttc ggcgtcatct ttcgccagtt
    28681 tgccggagat aagcatctcc acgcacagcg ccatcgtgac atcaaacaga cgcgggttac
    28741 gatactcacc cgtcaggaac tgcaccgctt cacgaacttc aaccgcgtta cctgcactgg
    28801 aggccagtac ctgattcatg tcggtgagca gcgcggtggt gcgcacgcca gcgccgttag
    28861 ccacgccaac aatcgcttcg gcaagggctt cagagagttc gtaggtcggc ataaacgcgc
    28921 cgctacccac tttcacgtcc atcaccagcg cgtccagacc ttccgcaagt ttcttcgcca
    28981 gaatagaggc ggtgatcagc gggatggagt ccacggttgc ggtaatatca cgggtcgcgt
    29041 agaaacgttt atccgccgga gccagtgagc tggtctgacc gataatcgcc acgccgacgt
    29101 ctttaataat ttcgcggaaa cggttgtcat ccgggaaaat gtcgaagcca gggatggatt
    29161 ccagtttgtc gagcgtaccg ccagtatgac cgaggccgcg accagagatc atcggaatat
    29221 agccgccgca ggctgcgacc atcggcccca acatcagcga agtcacatcg ccgacgccac
    29281 cagtggaatg tttatcaaca atcgggccat tcagatgcag gcttttccag tcgagaacgg
    29341 ttcctgaatc tcgcatcgcc atggtcagcg agacacgctc aggcattgtc atatcgtgga
    29401 agaaaatggt catcgcgagg gcggcaatct gcccttcgga gatagtgttg tcgcgaatgc
    29461 cgttgataaa gaaacgaatt tcttcatcgc tcagtgcatg accatcacgt tttttacgaa
    29521 taatttcttg tgcgagaaac aaggtaacct cccgaggaaa agagtatgag gcggcttttg
    29581 ccggatgcgt ctacgcctta tccggcctac gggtagatac gtctcgtagg cctgataagc
    29641 gcagcgcatc aggcgtaaag catcttactt agtagctgct ggcgctctta ccgtcaccgt
    29701 ggcccagcgc tttcaacagg cttgccagca ggctggaagc accaaagcgg tagtgacgcg
    29761 catctgccca gtcagcaccg aacagttcat ctgcgatggc gagatatttc tgcgcatctt
    29821 ccgcagtacg cacgccgccc gctggtttga aaccaacggt tttttctacg cccatatcac
    29881 ggatcacttc catcatgatg cgcgcgcttt ccggcgtcgc gttcacagcc actttaccgg
    29941 tagaggtttt gatgaagtcc gcacccgctt tgatggagat ttcagacgct ttacggatca
    30001 gcgcttcgtc tttcagttcg ccggtttcga tgatcacttt cagcagtaca ttcgctgccg
    30061 cgcaagcctc tttacaggct ttcaccaggt caaaaccaac ctgctcgtta cccgccatca
    30121 gcgcgcggta cgggaacaca acgtcaactt catcagcacc gtaggcgatt gccgcacggg
    30181 tttctgccag cgcgatttcg atgtcatcgt taccgtgtgg gaagttggtt accgtagcga
    30241 ttcggatttc cggggtgccc tgctctttca gcgttttgcg agcaatcggg ataaagcgag
    30301 gatagataca gatagcggcg gtattgccga ccggggtttt ggcctgatga cacagagcaa
    30361 ttactttctc gtcggtgtcg tcgtcattca gggtggtcag gtccatcaat ttcagtgcac
    30421 gcaggctgct tgctttcaga tcagtcattt cattctcctg gcttgtcgcc aataaaattc
    30481 accttgcgag tttgttagta ttctaacatc tactccgcaa cacacttcga tatacatcac
    30541 aattaaggaa atctacttac aagtttgcat cactgtaatg cgatctggtt caaataattc
    30601 actttcaaat gaatgcgtca gtggtggcaa acgcatcagg atcaaagtga acatcacgaa
    30661 acttcttaca atggcgcatc accttcggca taaaaggaaa agaagatgcc cacttctcat
    30721 gaaaatgcac tgcaacaacg ttgccagcaa attgtcacca gcccagtgct tagcccggag
    30781 cagaagcgcc attttctggc actggaagca gaaaacaatc tgccttatcc gcagcttcct
    30841 gccgaagccc gccgcgcgct ggatgagggt gtaatctgcg atatgtttga aggtcatgcg
    30901 ccgtacaaac cgcgctatgt cttacccgat tacgcccgtt ttctggcgaa cggttccgaa
    30961 tggctggagc tggaaggcgc gaaagatctt gatgacgcac tctctctgct gaccattctt
    31021 taccaccacg taccgtcggt cacatcgatg ccggtctacc tggggcaact ggatgcgttg
    31081 ttgcaaccat atgttagaat tctaacacaa gacgagatcg atgttcgaat aaaacgtttc
    31141 tggcgttacc tcgacagaac cctgccagac gcctttatgc acgccaatat cggcccgtct
    31201 gattcgccca ttacccgcgc gatcttacgt gcagacgcag agttgaagca ggtttcaccg
    31261 aacctgacct ttatctacga tcctgaaatc acccctgatg acctgctgct ggaagtggcg
    31321 aagaacatct gtgaatgtag caaaccgcac atcgccaacg gtccggtgca tgataaaatt
    31381 ttcacaaaag ggggctacgg gattgtgagc tgttacaact cactgccgct ggcgggtggt
    31441 ggcagcacgc tggtacgcct taacctgaaa gccattgccg agcgcagcga atcgctggat
    31501 gacttcttta cgcgcactct accgcactac tgccagcagc agatcgccat catcgatgcg
    31561 cggtgtgaat tcctctatca acaatcacac ttctttgaga atagcttcct ggtgaaagaa
    31621 gggctgatta accctgaacg ttttgtgcca atgtttggca tgtatgggct ggcggaagcg
    31681 gttaacttgc tgtgtaagaa agaagggatt gccgcgcgct acggtaaaga agccgccgca
    31741 aatgaagtgg gttatcgcat cagcgcgcaa ctggcggagt ttgtcgccaa tacccccgtg
    31801 aaatatggct ggcaaaaacg cgccatgtta cacgcacagt cggggagcag ttccgatatc
    31861 ggcaccacgc cgggcgcgcg tttgccgtat ggtgatgagc cagatccgat cacccatctg
    31921 caaactgtcg ccccgcatca tgcttattat tattccggca tcagcgacat tctgacgctc
    31981 gacgaaacca tcaaacgtaa tccgcaggcg ctggtacagc tttgcctcgg tgcctttaaa
    32041 gccggaatgc gtgaatttac tgccaatgtc agcggtaacg atctggttcg cgttaccggt
    32101 tatatggtgc gtttgtcgga tttagaaaaa tatcgcgccg aaggttcacg caccaacacc
    32161 acctggctgg gcgaagaagc cgcacgcaac actcgtattc tggaacgcca gccgcgcgtg
    32221 ataagccatg aacagcagat gcgctttagt cagtaagatt atccccttct cctgcgttga
    32281 cgggccaggc agtcgtctgg ctctgttttt gcagggctgc aatctgcgct gcaaaaactg
    32341 tcacaatccg tggacgatgg gacgttgcaa tgactgtggg gagtgcgtgc cacagtgtcc
    32401 gcatcaggcg ttgcagattg ttgacggcaa agtggtgtgg aacgctgtgg tttgcgagca
    32461 gtgtgatacc tgcctgaaga ggtgtccgca acatgccacg ccaatggcgc aatccatgag
    32521 cgtggacgaa gtgcttagcc atgtccgcaa agcggtgctg tttattgaag ggataacggt
    32581 aagcggcggc gaagccacga cccaactgcc gtttgtagtg gcgctgttta ctgctatcaa
    32641 aaacgatccg caactgcgcc acctcacctg cctggtggac agtaacggca tgttgagcga
    32701 aaccggctgg gaaaaattac tcccggtgtg tgacggcgca atgctcgatc tcaaagcgtg
    32761 ggggagcgaa tgtcatcaac acttaaccgg acgcgataat cagcagatta agcgcagcat
    32821 ctgtttgctt gcagagcgcg gcaagctggc ggaactgcgt ttgctggtaa ttccagacca
    32881 ggtggattat ttgcatcaca tcgatgagct ggcgacgttt atcaagagac ttggcgatgt
    32941 tccggttcgc ctgaatgcgt ttcatgccca cggcgtgtat ggcgaggcgc aaagctgggc
    33001 gagcgccacg ccggaagacg ttgagccgtt ggctgatgcg ttaaaggtgc gcggggtgag
    33061 ccggttgata tttccggcgc tctatttgtg acatgccgga tacggcctac ggttcggcac
    33121 agacttgtag gcatgataag acgcgacaag cgtcgcatca ggcattatgc gccgactgcc
    33181 ggatgcggcg tgaacgcctt atccggccta cggttcggca cagacttgta ggcatgataa
    33241 gacgcgtcag cgtcgcatca ggcattgtgc gccaactgcc ggatgcggcg tgaacgcctt
    33301 atccggccta cggttcggca caaaccagta ggcatgataa gacgcgtcaa gcgtcgcatc
    33361 aggcattgtg cgccaactgc cggatgcggc gtgaacgcct tatccggcct acggttcggc
    33421 acaaacctgt aggcatgata agacgcgtta agcgtcgcat caggcattat gcgccgactg
    33481 ccggatgctg cctacgaagg gtaggctacc cactaacgct aaacaacgca tacgtattat
    33541 taagcaacac ttcggcaatc tcatcttctg gttccgggcg caactcgcaa agcacgtcaa
    33601 acacccgcac agcctgttcc ggtcggttag gctgcccctg aaagccgttg agcggcatat
    33661 ccggcgcgtc ggtttccagc aacaacgatg ccagcggtaa ttttgcgatg acatcgcggg
    33721 ttttactggc gcgtggatag gtgatagtac cgcctacgcc gattttgtag cccagctgta
    33781 caaaccgctc ggcctgttgc aggctgccgg aaaaaccgtg aaccacgcca gtgcacggta
    33841 aatcgtgacg tttaagatgc atcgccagtt tgtcgtgtat acgccgggaa tgaaggatca
    33901 ccggcagatc gtagcgtttc gccagtttca gctgttcgtc gagtaaccac tgctgcctct
    33961 caaattgcgg atcgtcgccg aagagatcca gaccgatctc ccccaccgcc accaccttcg
    34021 ccggacgcct ttccagcgcc tgctgtagct gatcaagaga cacatcgcta tgtttttcca
    34081 acatccccgg atgcaacccc aatgcggcat acagcggttg atatttttcc gctaacgcct
    34141 gcacgcgggc aaaattttcc gcctctgtcg ccggaacaat gatcttgcct acgcccgctt
    34201 gtgccgcgcg ttgcaggctg gcctcttcat cgccactaaa cggcgggaaa tcaaaatggc
    34261 agtgggtatc gataaaacgg caaatcaagc cagatcctca ttgttaaatg tcgggtcgtt
    34321 cgcctgcgga gcatcgctca cttcggcaac cagcgtatcg ttggcgactg gcgcaggcgg
    34381 aataacgatc gattccggcg tcaccactgg caccagatgg cgggtaagcg gcgctttttc
    34441 agttaatagc ttgccaaccg tggcgaggaa ataacgaccg cataaacgcc caagtttata
    34501 gtcttcacgc agcgccggaa tccgactgcc aagcgcgata ctatgtaatg gcttcggcgg
    34561 ataaatttcg aatatccgca gcttgcccgg tggtttctca ataaattgct gaatgtcacg
    34621 atagctggtt tcatgatgct gcaccagatt gaccagcggc tgcaggctac tgtcacccag
    34681 ccagcgttcc atgcgtttga accactgcgg cgtgtagtac atttgtgacg gcacagtgcg
    34741 aatgacgacc aacgttttag cgccctgcct tgccgcctct ttaaccggaa tcgcatcact
    34801 gatcccgcca tccaggtagt taatgccttc cagcgacact ccgctacgat aaaagccagg
    34861 tatcgcactg gaggcgcgaa tcacatccag ccagttttgt ttggttggta aaaagtaatt
    34921 aggcgcgtag tcatcctgac gacaggcgca catataaaac gatttgccgc tgtcaaacaa
    34981 ccgcgcggcg gtatccattt gcaacggcat ctggcttgcg gtggcctcca ccagccagtc
    35041 gagatcgata agatttcctc cacggacaaa gcgcaatgga tcgaaaaatt cgcgttttgt
    35101 ggtatagcgc atgatgactt tgcgcgcgta accgggctga ttgcagataa acgccgagag
    35161 gttctgcgcc ccggcagatg tgccgagata aagatcgaaa ggattaaact gcgcgcgcat
    35221 aaactcatcc agcacgccag ccgtgaaaat tccacgctgt ccgccgcctt cgcacaccag
    35281 agctattcgt ccaggctgga acggccttag cgacaacggc gcaatattac caagcgttac
    35341 aggtattcgc tgccccacct ctgccttcct gtttttgttg tgaaagaatg acaaagtaac
    35401 gcaatttatt attcgctaaa accgcaaaag ccagtccgct ggactggctt tggataaaat
    35461 tttcttaatg gtctgaaatc tagggtcgtt ttcggcccat gaacaaactc accaggaaca
    35521 gaataatccc gacgacaaag acaattttag ctgcgcctgc agcggtaccg gccagaccac
    35581 caaacccaag tgcggcggcg attaacgcga taaccagaaa tatgatgccc caacgaaaca
    35641 taagcgtctc ctttaccata gttaatgtca ccgcttgata tgagcgtgaa aatcactcaa
    35701 tgcgatattt ttagtgtggt gcacatcgcg cctcccgaca aagttcggga ggacgaatta
    35761 cgacgaatta cttagttttc agatcatttt taacgctttt cacaccatct accgctttgg
    35821 cgatactttc agcacggtcg ctctgtgcct gggaatcgac ggtaccggag agctgaacca
    35881 cgccgtcggt ggtttcaact ttcacatgac gggaagggac gatatcgtcc gccagcagtt
    35941 tggctttgat ttcactggtg gtggcggtgt cacccgcgta gcccttcaca gagccttctt
    36001 tagcgtcgcg aacgtgcagt ttgtcgctga cagaggtcac cccttcaacg cctttcgcca
    36061 ctttcactgc ctcttcggcc tgagcctggc tttcaacgaa accgctcagg gtcacgactt
    36121 tttgatcggt tttgacagag atatcggtgc tcttgatgtt gtcatgatcc accagcgccg
    36181 ccttcacttt cgcggtgatg gcgctgtcat ccatgaaatt accgacttta ttcatagagc
    36241 tatcgacttt ttgccctgcg ctttcattgg tagtctgtgc gttgttttcc gcgtaggcag
    36301 agccggtcgc gacggcagag gtcaacatta cagccagcag agttttcgaa atcttcagtc
    36361 ttgtcatagt catcgattta ttcctgtatg tttgctcgta atttgagcct ggcaacacga
    36421 ggttgcattg ctgaataggg agagacttca ccctctacag aagtcaatgg tcgccatcac
    36481 aaaagcgatg ggtgatgaat aacgaccatt acagcctctg aatcagttat taatatcggt
    36541 agaatgacaa tcgacggctt tagatactga tatctacgca ttgaacggta tttaacgccg
    36601 tcagaaatgt cattactttg ttaaatatag atcacaattt tgaaaccgct cgggatatca
    36661 gcgaaaacat aagcaaaagt gaatgtttta agaacattcc gtaagcggct aataaggaag
    36721 ggaaattgac agggcgcagc ggctaccgcg ccctggaggc aagaattaat gctcgcgggt
    36781 ctggtggaac tgaacgtcgg gataacgttc ctgcgccagg cgcaggttga ccatgctggt
    36841 agcgatgtaa gcgaggttat cgccgccatc aagcgccagt tggctttcgt tcttacgctt
    36901 gaactcttcg aatttcttcg cgtccgcaca ttctacccag cgggcagtgg caacgttgac
    36961 tgattcatat actgcttcaa cgctgtattc gctcttcagg cgcgatacca ccacatcaaa
    37021 ctgcagcaca ccaacagcac caacgatcaa atcgttgttg gagatcggac ggaacacctg
    37081 caccgcgccc tcttcggaaa gctgtaccag ccctttgagc agctgttttt gcttcagcgg
    37141 atctttcagg cggatacgac ggaacagttc tggtgcgaag ttcggaatac cggtgaactt
    37201 catcatctca ccctgggtaa aggtgtcgcc gatctgaata gtgccgtggt tgtgcaggcc
    37261 gaggatatcg cccggatacg cttcttcaac gtgcgaacgg tcgcccgcca taaaggtcag
    37321 cgcgtcggag ataaccacat ctttcgcagt acgcacctgg cgcagcttca tgcctttttc
    37381 atatttaccg gatactacgc gcataaacgc cacgcggtcg cggtgtttcg ggtccatgtt
    37441 ggcctgaatt ttaaatacga agccggtaaa tttgtcttcg ctcgcctcta cggtacgggt
    37501 atcagtctga cgcggcatcg gcgcaggagc ccactccacc aggccatcca gcatatgatc
    37561 aacgccgaag ttacccagcg cagtaccgaa gaataccgga gtgatttcgc ccgcaaggaa
    37621 caattctttg tcgaactcgt tagacgcgcc tttcaccagt tccagttcgt cacgtagctg
    37681 ctgtgccaga tcttcaccaa ccgcagcgtc gagatccggg ttattcagcc ctttaacaat
    37741 acggacttcc tgaatcgtgt ggcctttacc gctctgatag agataggttt cgtctttata
    37801 aaggtggtaa acgcctttaa acagcttgcc gcagccaatc ggccaggtga tcggtgcgca
    37861 gccgattttc agctcgttct caacttcatc gagcagttcc atcgggtcgc ggatatcacg
    37921 gtcaagtttg ttcataaagg tgaggatcgg cgtgtcgcgc agacgggtaa cttccatcag
    37981 cttacgggta cgatcttcaa caccttttgc agcgtcgata accatcaggc agcagtccac
    38041 cgccgtcagg gtacgatagg tatcttccga gaagtcttcg tgccccgggg tgtcgagcag
    38101 gttaaccagg caatcgtgat acggaaactg catcacagac gtagtaatgg agatcccacg
    38161 ctgcttttcc atctccatcc agtccgactt agcgtgctgg ttggaaccac ggccttttac
    38221 tgtaccggcg gtctgaatgg cctgtccgaa cagcagcacc ttctcggtga tggtagtctt
    38281 accggcatcc gggtgagaaa taatggcaaa agtgcggcgc ttcgccacct cttgcaaata
    38341 aggagacaac gtcataattc tttcttcttg agtaagcggc atcgcgccgc gcttgttgaa
    38401 aacgaaaaat tgcggctatt ttacccatca acggggggga ggcaatcagt gtttacacag
    38461 gagctgctcc agttcgtgca acgaagaaac ggtccaggtg ggcgcgatgc cttctggttg
    38521 ctcgcgattg tgcgcattca gccagcaggt cgcaagcccg gcgttgatgc caccgagaat
    38581 atcggactcg gcagtgtcgc caaccatcag cacgcgtgaa cggtcaggat tgcccgcctg
    38641 ttccagcgca taatcgaaaa ttttcttatt cggtttggca acgccaactt cttcggaaat
    38701 caccagcaaa tcgaagtaat cacgcaggcc cgtgcgttcc agacgcactt gctgcaaggc
    38761 gctaaagccg ttggtgatga tgccgatttt ggcgttgcca cgaatggcgt taagcagaga
    38821 aaccgcgccc ggcagcggcg tgcagatttc cgccatcgca ttaataaagg catcgttaag
    38881 caagcctgct tcaactttca gccgttcggc ccagctctca aaacgcccgt gctgaagctg
    38941 taatgaagtg atcgcgccgt tttgataatc cacccacagt ggcttgttaa cggcctgata
    39001 gtcctgaaaa tcttcagcgg taaaggtgac gctgtaatca agaaacatcc gctgcaggcc
    39061 ggtgaatgag tcaaaggtaa acagcgtttc atcggcatca aagaaaatcc agtcccactt
    39121 cattattcca ccttgtctta catactgatt ggcaacgcca tgatgatggc gtcttcgcga
    39181 ccgtccgtgg tggggtagta attgcggcga atcgtcgcct cgttaaagcc taaactttcg
    39241 tacagggcaa tggcggcagc atttgaggca cggacttcca gccatagtgt cgccacgccg
    39301 cgtttttcca gttcgtcgat cagatgttcc agcagcaccc ttcccaatcc ctgacgctga
    39361 tagtcaggat cgaccgcaat attgaacaat gtagcttcat ccagcaccac ttgcgtaatc
    39421 gcaaacgccg ccattttgcc gttttgcgtt aactgaaagt tgagataacg ctcgccctgg
    39481 ttgctggcaa aggttttttc actccacgga aaggcgtggg cgcgttgttc aatgtggtaa
    39541 gccgctggta aatcagtcgt ttcgagggaa gaaatcgtgt tcatatgtgc aaatctgttg
    39601 ccataacgcg gcgcgtgccg ttgggtttgc ccgtaattcg gtgagcgccg gtgatgccac
    39661 ctgagcgcct tccagtgata gcggttcgtc agtacccaac cgccagctgt tgcagcgact
    39721 gccttgcggc agcatcgcga ttttttctgg cgtcagttgc agcacttgat cggggctgac
    39781 ggttaatgcg cgcagaacat cgctcactaa aggatcggtc agggcgggaa gatcgtttgc
    39841 caccatcacc agacggacgt gtgccgggat cgcaatggca atctccccct gcaacgcgcc
    39901 agggcgacgc agcgaccact gggtaatgcc cagttgctgt aactgccagt ctcgtcggga
    39961 tgtcatagcg aatcgctcct gttgtcaggg gcgcaaatat agcaaattcg tcgataccgc
    40021 gccaacatat ggctataatc gccgccagta tcaattgagg agcattccat gtctgcattt
    40081 accccggcaa gtgaagtctt gctgcgtcac agtgatgatt tcgaacaaag ccgtattctg
    40141 tttgccggag acttacagga tgacctgccc gcgcgtttag ataccgcggc cagccgtgct
    40201 catacccagc aattccacca ctggcaggta ttaagccgcc agatggggga taacgcccgt
    40261 ttcagtctgg tcgccacggt ggatgacgtc gcagattgcg atatgctgat ttactactgg
    40321 ccgaagaaca aaccggaagc ccagttccag ttgatgaatt tactttctct gctgccagtg
    40381 gggacagata tttttgtcgt tggcgagaac cgcagcggcg tgcgcagcgc cgagcagatg
    40441 ctggcagatt atgcgccgtt gaataaagtc gacagcgctc gtcgctgtgg cctctatttt
    40501 ggtcgtctgg aaaaacagcc ggtatttgat gccgataaat tctggggcga atacagcgtc
    40561 gatggcctga cggtcaaaac gctgcctggc gtgtttagcc gcgacggtct ggatgtcggt
    40621 agccagttgc tgctctcgac gttaactccg cacacgaaag gtaaagtgct ggatgtcggc
    40681 tgtggcgcgg gggtgctttc agttgccttt gcgcgccatt cgccgaaaat tcgtctcacc
    40741 ttgtgcgatg tctctgcgcc agcggtagaa gccagccgcg caacacttgc ggccaactgt
    40801 gttgaaggtg aagtctttgc cagcaacgtc ttttccgagg tgaaaggtcg ttttgatatg
    40861 atcatctcca acccgccgtt ccacgatggg atgcaaacca gcctggatgc ggcgcaaacg
    40921 ctgattcgcg gcgcggtgcg tcatcttaat agcggcggcg agctgcgaat tgtagcgaac
    40981 gccttcctgc cttacccgga cgtgctggat gagacatttg gcttccatga agtgattgcg
    41041 caaaccgggc gtttcaaggt gtatcgcgcc attatgaccc gccaggcgaa gaaaggttaa
    41101 ttatctcgcc gaataccgtg tcggatgcgg cgtgaacgcc ttatccgacc tacaaaacct
    41161 gcacgttagc ccttcgtagg ccagataaga cgcgccagcg tcgcatctgg catctgcccc
    41221 cgcatgccta ccaaacctgc acgctagttt cctgatggac atttttccag caattacacc
    41281 tccggcgata attaactatt gacgaaaagc tgaaaaccac tagaatgcgc ctccgtggta
    41341 gcaattcttt ttaagaattg atgg
//
LOCUS       DAJTZL010000011        40917 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.011, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000011 DAJTZL010000000
VERSION     DAJTZL010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40917
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.011"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            20..931
                     /locus_tag="OO544_000555"
     CDS             20..931
                     /locus_tag="OO544_000555"
                     /inference="COORDINATES: protein motif:HMM:TIGR01643.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4329 domain-containing protein"
                     /protein_id="HCR6766243.1"
                     /translation="MKKEHADGSVTQSQFDAVGRLRTQTDAAGRTTEYSPDVVTGLIT
                     RITTPDGRASAFYYNHHSQLTSATGPDGLEIRREYDEWGNLLNEENPHQLQQLIRLPG
                     QQYDEESGLYYNRHRYYDPLQGRYITQDPIGLKGGWNLYTYPLSPVNSMAPLGLYEFK
                     SKNIDDIGIFALAMCNGESINENKEYGGLICKKQGEYFPMNPISSNDNDSVDLRNIKC
                     PEGSERVGDYHTHGFYSDDKGNKVTKENDVYDSLNFSSKDLTNSYMNGMGKKEYSSYL
                     GTPNNTYLKYNPKAKGNGVTIIRQGSN"
     gene            933..1169
                     /locus_tag="OO544_000556"
     CDS             933..1169
                     /locus_tag="OO544_000556"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6766244.1"
                     /translation="MKAIQCCYFLALVSSHLMAGVMGDDNIFLSNSDSAALYCVAKIF
                     PDGENILIYKVNIINASSKKDVFFMKAQHQPTMT"
     gene            complement(1342..2017)
                     /locus_tag="OO544_000557"
                     /pseudo
     CDS             complement(1342..2017)
                     /locus_tag="OO544_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(2162..2770)
                     /locus_tag="OO544_000558"
     CDS             complement(2162..2770)
                     /locus_tag="OO544_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HCR6766245.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVIRNKKPYVFEAVGPVKYPPLKQWIAHGEKGKYVVRRVEGGLSVEQQQK
                     LAQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(2954..3271)
                     /gene="metJ"
                     /locus_tag="OO544_000559"
     CDS             complement(2954..3271)
                     /gene="metJ"
                     /locus_tag="OO544_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HCR6766246.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            3548..4708
                     /gene="metB"
                     /locus_tag="OO544_000560"
     CDS             3548..4708
                     /gene="metB"
                     /locus_tag="OO544_000560"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HCR6766247.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLSGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            4711..7143
                     /gene="metL"
                     /locus_tag="OO544_000561"
     CDS             4711..7143
                     /gene="metL"
                     /locus_tag="OO544_000561"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HCR6766248.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTDFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            7492..8382
                     /gene="metF"
                     /locus_tag="OO544_000562"
     CDS             7492..8382
                     /gene="metF"
                     /locus_tag="OO544_000562"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HCR6766249.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            8711..10891
                     /gene="katG"
                     /locus_tag="OO544_000563"
     CDS             8711..10891
                     /gene="katG"
                     /locus_tag="OO544_000563"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HCR6766250.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSERVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            10985..11890
                     /gene="yijE"
                     /locus_tag="OO544_000564"
     CDS             10985..11890
                     /gene="yijE"
                     /locus_tag="OO544_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HCR6766251.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVMALALVSRKKKEAVSVKRI"
     gene            complement(11917..12534)
                     /locus_tag="OO544_000565"
     CDS             complement(11917..12534)
                     /locus_tag="OO544_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HCR6766252.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYMQLTYPGGDVPQKRGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWQLKRPDSNIDHRRVPNLETWFTHHDKTRPISKNPSDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(12808..13911)
                     /gene="gldA"
                     /locus_tag="OO544_000566"
     CDS             complement(12808..13911)
                     /gene="gldA"
                     /locus_tag="OO544_000566"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HCR6766253.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGGCSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAECE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            complement(13922..14584)
                     /gene="fsa"
                     /locus_tag="OO544_000567"
     CDS             complement(13922..14584)
                     /gene="fsa"
                     /locus_tag="OO544_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HCR6766254.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSEGLAAIKILKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDALGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            complement(14596..17097)
                     /gene="ptsP"
                     /locus_tag="OO544_000568"
     CDS             complement(14596..17097)
                     /gene="ptsP"
                     /locus_tag="OO544_000568"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR6766255.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLHTDRK
                     GNAKSALALIGTDTLAGDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALSNLPAAKDVDAEQSTLE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLRQHLLAGVSEGLSCAEAIVAS
                     ANHFCEEFARSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQSAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLWALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            17406..18485
                     /locus_tag="OO544_000569"
     CDS             17406..18485
                     /locus_tag="OO544_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HCR6766256.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGTWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            18500..18820
                     /locus_tag="OO544_000570"
                     /pseudo
     CDS             18500..18820
                     /locus_tag="OO544_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
     gene            18871..21167
                     /locus_tag="OO544_000571"
                     /pseudo
     CDS             18871..21167
                     /locus_tag="OO544_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
     gene            21133..22011
                     /locus_tag="OO544_000572"
     CDS             21133..22011
                     /locus_tag="OO544_000572"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HCR6766257.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            22013..22354
                     /locus_tag="OO544_000573"
     CDS             22013..22354
                     /locus_tag="OO544_000573"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HCR6766258.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(22341..23192)
                     /locus_tag="OO544_000574"
     CDS             complement(22341..23192)
                     /locus_tag="OO544_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6766259.1"
                     /translation="MYHGVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(23407..25140)
                     /locus_tag="OO544_000575"
     CDS             complement(23407..25140)
                     /locus_tag="OO544_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HCR6766260.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            complement(25323..27974)
                     /gene="ppc"
                     /locus_tag="OO544_000576"
     CDS             complement(25323..27974)
                     /gene="ppc"
                     /locus_tag="OO544_000576"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HCR6766261.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            complement(28326..29477)
                     /gene="argE"
                     /locus_tag="OO544_000577"
     CDS             complement(28326..29477)
                     /gene="argE"
                     /locus_tag="OO544_000577"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HCR6766262.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASIGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDTLRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPSMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIHHFCWH"
     gene            29631..30635
                     /gene="argC"
                     /locus_tag="OO544_000578"
     CDS             29631..30635
                     /gene="argC"
                     /locus_tag="OO544_000578"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HCR6766263.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQVLQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            30643..31419
                     /gene="argB"
                     /locus_tag="OO544_000579"
     CDS             30643..31419
                     /gene="argB"
                     /locus_tag="OO544_000579"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HCR6766264.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSTLVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            31480..32853
                     /gene="argH"
                     /locus_tag="OO544_000580"
     CDS             31480..32853
                     /gene="argH"
                     /locus_tag="OO544_000580"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HCR6766265.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALSVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLDELQKFSPVIGEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FAQARLG"
     gene            33120..34037
                     /gene="oxyR"
                     /locus_tag="OO544_000581"
     CDS             33120..34037
                     /gene="oxyR"
                     /locus_tag="OO544_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HCR6766266.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGYFDKVLKQAV"
     gene            complement(34020..35420)
                     /gene="sthA"
                     /locus_tag="OO544_000582"
     CDS             complement(34020..35420)
                     /gene="sthA"
                     /locus_tag="OO544_000582"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HCR6766267.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGVRVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            35741..36391
                     /gene="fabR"
                     /locus_tag="OO544_000583"
     CDS             35741..36391
                     /gene="fabR"
                     /locus_tag="OO544_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HCR6766268.1"
                     /translation="MMGIRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            36391..36750
                     /locus_tag="OO544_000584"
     CDS             36391..36750
                     /locus_tag="OO544_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HCR6766269.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(36790..37890)
                     /gene="trmA"
                     /locus_tag="OO544_000585"
     CDS             complement(36790..37890)
                     /gene="trmA"
                     /locus_tag="OO544_000585"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HCR6766270.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IELDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPCILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      38004..38194
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            38259..40103
                     /gene="btuB"
                     /locus_tag="OO544_000586"
     CDS             38259..40103
                     /gene="btuB"
                     /locus_tag="OO544_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HCR6766271.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRVEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRGTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            40048..40905
                     /gene="murI"
                     /locus_tag="OO544_000587"
     CDS             40048..40905
                     /gene="murI"
                     /locus_tag="OO544_000587"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCR6766272.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         9448 a        10195 c        11261 g        10013 t
ORIGIN      
        1 agccgggctg aagcgggtgg tgaaaaagga acacgcggac ggcagcgtca cgcagagtca
       61 gtttgacgcc gtgggcaggc tcaggacaca gacggatgcc gcaggccgga caacagaata
      121 cagcccggat gtggtgacgg gtctcatcac gcgcatcacc acgccggatg gcagggcatc
      181 ggcgttttac tataaccacc acagccagtt aacgtcagcc accgggcctg acgggctgga
      241 aatacgccgg gaatatgatg aatggggcaa cctgctgaat gaagagaacc cgcatcagct
      301 gcagcagctt attcgtttgc cggggcagca gtatgatgag gagtccggcc tgtattataa
      361 ccgccaccgc tattatgacc cgctgcaggg gcggtatatc actcaggatc cgattgggct
      421 gaagggggga tggaacctgt atacatatcc gctgagcccg gtgaatagca tggctccatt
      481 aggattatat gaatttaaat caaaaaatat tgatgatatt ggaatatttg cattggcaat
      541 gtgtaatgga gaatcaatta acgagaataa agaatatggt ggactaatat gtaagaagca
      601 aggtgaatat ttccccatga atccgataag ttcaaatgat aatgatagtg tagacttgcg
      661 aaatataaaa tgccctgaag gttcagagag agtaggcgat tatcacactc acggttttta
      721 ctctgacgat aaaggaaata aagtaacaaa agaaaatgat gtttatgata gtctaaattt
      781 ttcaagcaaa gatttaacga attcttatat gaatggaatg ggaaaaaaag aatacagtag
      841 ttacttggga acaccaaata acacctatct aaaatataat cccaaagcta aagggaatgg
      901 agttacaatt atcaggcaag ggagtaatta aaatgaaggc aatacaatgt tgttatttcc
      961 ttgctttagt ctccagtcat ttaatggctg gagttatggg tgatgataat atatttttaa
     1021 gtaattcgga ttcagctgct ttatattgtg tggctaaaat atttccagat ggagaaaata
     1081 ttttaatata taaagtaaat ataattaatg ctagttcaaa aaaagatgtt ttttttatga
     1141 aagcccaaca tcaacccaca atgacataat ggggggcggg ggaatgtcat atgcctatga
     1201 atatgatgta aaatataata tatgtcattt gagaaaaaag agcagaatga ggtaaaaatc
     1261 tttggctgtg tattcagtct ggtaatcatc caaggaaagc ttatcagaag ggtgatgact
     1321 ccaattagta gaacatatat tttcgataaa agcaccgatg actttttcat gaagttctac
     1381 agaacgcgag aaacagatgg ttctgcgggc cagacgtttg attcgggttc tcagggtcag
     1441 attgttacgc tcaatgtgct gagtaaaaat cttaccagtc agatgcttct ccttcggtaa
     1501 ctctctagcg taactacccc attcgtcagt ggtcaacatg ccgatggtaa aagggtaagc
     1561 agcgcccgca attcacgaca ggtttcgtca gtccgggggc caaaagtgta ggccagaaca
     1621 ccaccagttt tcgtgttgta cacgtaccag agccagtgct gtcgcgcttt actgccgaca
     1681 aagctccatt gctgactaag tttgcagatg agtgccacat cagcatgagc gaccggggat
     1741 gacgttattc ttcgtggcga gagtttttta aagttcggat gactgtatta atgccgattt
     1801 tcagcgtacg ggcagtatcg cgaaccccgg caccgttgaa agtcatttca gcgatttggt
     1861 ctttaactcc cggtttacgg ggctcgtaac agtaactgag ctgaaatacc cggtgacagt
     1921 cacggccgcg gaatcgctca tgtctttttg ggttctgccc gtgacggtaa acctgaacgg
     1981 actggcaacg ggggcaatga aggttaactg tgaccatgaa acaaccttaa aagcgagtat
     2041 tatatcctta attcaactaa ttggaggcat caccgtttac cggagcgaca gcttgcggaa
     2101 aggtcgtgca actacaaagc aacaaacaga ctacggcggg aattattttt ttcatcatcc
     2161 attaccacga aaacagcggc cactctttgg cgactgtggt gagttgtggc gcgtcgaaaa
     2221 cggcttgtgg tgaaactacc gtttcctcaa gcggaatatt tttgccgtag cgttctttga
     2281 gcttagcctg gaccagcggg ttgctgagat cgaactcttt caacttctgc tgctcgccca
     2341 cgcgcattcc cagcgcgttc tggtaaacct tccacaccac ttccgaacag tactggcgat
     2401 cgtcgctcca tgagaagctg aaatcatacg gtttaccgag ataacgtttt gccgtttgcg
     2461 ccagtttttg ctgttgttca acactcagtc cgccttcaac gcggcgaaca acgtatttgc
     2521 ccttttcacc atgggcgatc cactgcttga gcggggggta tttcaccggg ccgaccgctt
     2581 caaaaacgta gggctttttg ttgcgtatca ccagcatacc ggtgtggcta taatcggaat
     2641 gggtcgccag ttggatcgct ttactttgcg atgagcgaga gatctgaaag atgatgtcgc
     2701 cggtttgtgg ctgccaggca aaggcaggta cagaaaccag caggctgagg atcagcagcc
     2761 tgtttttcat agttaaacgt ccatgtataa aaagcggtgg gtcgcagaca acgtgctcgt
     2821 tgtttatgcc ggatgcggcg tgaacgcctt atccggccta caagttcgtg caaattcaat
     2881 aaattgcaat atgacgtagg cctgataagc gtagcgcatc aggcgattcc actccgcgcc
     2941 gctctttttt gctttagtat tcccacgtct ccgggttaat ccccatctca cgcatgatct
     3001 cttttgccgc ttccgggatt tcgtcgctgc gctctttacg cagatcggca tcatccggca
     3061 aaggttgccc ggtaaaggca tgcagaaacg cttcgcacag cagctcgctg ttggtagcgt
     3121 gacgcaggtt gttcacctga cgacgcgtgc gttcatcggt gaggattttt aacaccttaa
     3181 gaggaatgga aaccgtaatc tttttgactt gttcactctt cttgccgtgc tcagcgtatg
     3241 ggctgatata ttcgccgctc cattcagcca tgagatactt aatcctcttc gtcaataaat
     3301 tgagaccaga ccacacaatt tatgtgggta ctgaccgtaa acccgcatag tttaccgtac
     3361 aggcgttacc gtgacatcgt gtaatgcacc tgtcggcgtg ataatgcata taattttaac
     3421 ggctatttgg gatttgctca atctatacgc aaagaagttt agatgtccag atgtattgac
     3481 gtccattaac acaatgttta ctctggtgcc tgacatttca ccgacaaagc ccaggaaact
     3541 tcatcacatg acgcgtaaac aggccaccat cgcagtgcgt agcgggttaa atgacgacga
     3601 acagtatggt tgcgttgtcc ctcccatcca tctttccagc acctataact ttaccggatt
     3661 taatgaaccg cgcgcgcatg attactcgcg tcgcggcaac ccaacgcgcg atgtggttca
     3721 gcgtgcgctg gcagaactgg aaggcggtgc tggcgcagta cttactaata ccggcatgtc
     3781 cgcgattcac ctggtaacga ccgtcttttt gaaacctggc gatctgctgg ttgcgccgca
     3841 cgactgctac ggcggtagct atcgcctgtt cgacagtctg gcgaaacgcg gttgctatcg
     3901 cgtgttgttt gttgatcaag gcgatgaaca ggcattacgg gcagcgctgg cagaaaaacc
     3961 caaactggta ctggtagaaa gcccaagtaa tccattgtta cgcgtcgtgg atattgcgaa
     4021 aatctgccat ctggcaaggg aagtcggggc ggtgagcgtg gtggataaca ccttcttaag
     4081 cccggcatta caaaatccgc tggcattagg tgccgatctg gtgttgcatt catgcacgaa
     4141 atatctgaac ggtcactctg acgtggtggc tggcgtggtg attgctaaag acccggacgt
     4201 tgtcactgaa cttgcctggt gggcaaacaa tattggcgtg acgggcggcg cgtttgacag
     4261 ctatctgctg ctacgcgggt tgcgaacgct ggtgccgcgt atggagctgg cgcagcgcaa
     4321 cgcgcaggcg attgtgaaat atctgcaaac ccagccgttg gtgaaaaaac tgtatcaccc
     4381 gtcgctgccg gaaaatcagg ggcatgaaat tgccgcgcgt cagcaaaaag gctttggcgc
     4441 aatgttgagt tttgaactgg atggcgatga gcagacgctg cgtcgtttcc tgagcgggct
     4501 gtcgttgttt acgctggcgg aatcattagg gggagtggaa agtttaatct ctcacgccgc
     4561 aaccatgaca catgcaggca tggcaccaga agcgcgtgct gccgccggga tctccgagac
     4621 gctgctgcgt atctccaccg gtattgaaga tggcgaagat ttaattgccg acctggaaaa
     4681 tggcttccgg gctgcaaaca aggggtaaaa atgagtgtga ttgcgcaggc aggggcgaaa
     4741 ggtcgtcagc tgcataaatt tggtggcagt agtctggctg atgtgaagtg ttatttgcgt
     4801 gtcgcgggca ttatggcgga gtactctcag cctgacgata tgatggtggt ttccgccgcc
     4861 ggtagcacca ctaaccagtt gattaactgg ttgaaactaa gccagaccga tcgtctctct
     4921 gcgcatcagg ttcaacaaac gctgcgtcgc tatcagtgcg atctgattag cggtctgcta
     4981 cccgctgaag aagccgatag cctcattagc gcttttgtca gcgatctgga acgcctggcg
     5041 gcgctgctcg acagcggtat taacgacgca gtgtatgcgg aagtggtggg ccacggggaa
     5101 gtatggtcgg cacgcctgat gtctgcggta cttaatcaac aagggctgcc agcggcctgg
     5161 cttgatgccc gcgagttttt acgcgctgaa cgcgccgcac aaccgcaggt tgatgaaggg
     5221 ctttcttacc cgttgctgca acagctactg gtgcaacatc cgggcaaacg tctggtggtg
     5281 accgggttta tcagccgtaa caacgccggt gaaacggtgc tgctggggcg taacggttcc
     5341 gactattccg cgacacaaat cggtgcgctg gcgggtgttt ctcgtgtaac catctggagc
     5401 gacgtcgccg gggtatacag tgccgacccg cgtaaagtga aagatgcctg tctgctgccg
     5461 ttgctgcgtc tggatgaagc cagcgaactg gctcgtctgg cggctcccgt tcttcacgcc
     5521 cgtactttac agccggtttc tggcagcgaa atcgacctgc aactgcgctg tagctacacg
     5581 ccggatcaag gttccacgcg cattgaacgc gtgctggcct ccggtactgg tgcgcgtatt
     5641 gtcaccagcc acgatgatgt ctgtttgatt gagtttcagg tgcccgccag tcaggatttc
     5701 aaactggcgc ataaagagat cgaccaaatc ctgaaacgcg cgcaggtacg cccgctggcg
     5761 gttggcgtac ataacgatcg ccagttgctg caattttgct acacctcaga agtggccgac
     5821 agtgcgctga aaatcctcga cgaagcgggg ttacctggcg aactgcgcct gcgtcagggg
     5881 ctggcgctgg tggcgatggt cggtgcaggc gtcacccgta acccgctgca ttgccaccgc
     5941 ttctggcagc aactgaaagg ccagccggtc gaatttacct ggcagtccga tgacggcatc
     6001 agcctggtgg cagtactgcg caccggcccg accgaaagcc tgattcaggg gctgcatcag
     6061 tccgtcttcc gcgcagaaaa acgcatcggc ctggtattgt tcggtaaggg caatatcggt
     6121 tcccgttggc tggaactgtt cgcccgtgag cagagcacgc tttcggcacg taccgacttt
     6181 gaatttgtgc tggcaggcgt ggtggacagc cgccgcagtc tgttgagcta tgacgggctg
     6241 gacgccagcc gcgcgttagc cttcttcaac gatgaagcgg ttgagcagga tgaagagtcg
     6301 ttgttcctgt ggatgcgcgc ccatccgtat gatgatttag tggtgctgga cgttaccgcc
     6361 agccagcagc ttgctgatca gtatcttgat ttcgccagcc acggtttcca cgtcatcagc
     6421 gccaacaaac tggcgggagc cagcgacagc aataaatatc gccagatcca cgacgccttc
     6481 gaaaaaaccg ggcgtcactg gctgtacaat gccaccgtcg gtgcgggctt accgatcaac
     6541 cacaccgtgc gcgatctgat cgacagcggc gatactattt tgtcgatcag cgggatcttc
     6601 tccggcacgc tctcctggtt gttcctgcaa ttcgacggta gcgtgccgtt taccgagctg
     6661 gtggatcagg cgtggcagca gggcttaacc gaacctgacc cgcgtgacga tctctctggc
     6721 aaagacgtga tgcgcaagct ggtgattctg gcgcgtgaag caggttacaa catcgaaccg
     6781 gatcaggtac gcgtggagtc gctggtgcct gcccattgcg aaggcggcag catcgaccat
     6841 ttctttgaaa atggcgatga acttaacgag cagatggtgc aacggctgga agcggcccgc
     6901 gaaatggggc tggtgctgcg ctacgtggcg cgtttcgatg ccaatggcaa agcgcgtgtg
     6961 ggcgtggaag cggtgcgtga agaccatccg ctggcatcac tgctgccgtg cgataacgtc
     7021 tttgccatcg aaagccgctg gtatcgcgat aatccgctgg tgatccgtgg gcctggcgct
     7081 gggcgcgacg tcaccgccgg ggcgattcag tcggatatca accggctggc acagttgttg
     7141 taatttcaga aatttaataa tgcccggtac tcatgttttc gggtttatgg tttctaatga
     7201 aatatattga attatcatag gattaggccg gattaagcgt ttacgacgaa tccggcaaga
     7261 agcaataagt acatggttag tttatatttg cagtccggtt tgctttgcat accggatttt
     7321 ctttttctta ccatcctgaa gttttttcat cttccctgat ttttcctcac catcattggt
     7381 catttttcgg ttgacgccct tcggcttttc cttcatcttt acatctggac gtctaaacgg
     7441 atagatgtgc acaacacaac atataactac aagcgattga tgaggtaagg tatgagcttt
     7501 tttcacgcca gccagcggga tgccctgaat cagagcctgg cagaagtcca ggggcagatt
     7561 aacgtttcgt tcgagttttt cccgccgcgt accagtgaaa tggagcagac cctgtggaac
     7621 tccatcgatc gccttagcag cctgaaaccg aagtttgtat cggtgaccta tggcgcgaac
     7681 tccggcgagc gcgaccgtac gcacagcatt attaaaggca ttaaagatcg cactggtctg
     7741 gaagcggcac cgcatcttac ttgcattgat gcgacgcccg acgagttgcg caccattgca
     7801 cgcgactact ggaataacgg tattcgtcat atcgtggcgc tgcgtggcga tctgccgccg
     7861 ggaagtggta agccagaaat gtatgcttct gacctggtga cgctattaaa agaagtggca
     7921 gatttcgata tctccgtggc ggcgtatccg gaagttcacc cggaagcaaa aagcgctcag
     7981 gcggatttgc ttaatctgaa acgcaaagtc gatgccggag ccaaccgcgc gattactcag
     8041 ttcttcttcg atgtcgaaag ctacctgcgt tttcgtgacc gctgtgtatc ggcgggcatt
     8101 gatgtggaaa ttattccggg aattttgccg gtatctaact ttaaacaggc gaagaaattt
     8161 gccgatatga ccaacgtgcg tattccggcg tggatggcgc aaatgttcga cggcctggat
     8221 gatgatgccg aaacccgcaa actggtgggc gcgaatattg ccatggatat ggtgaagatt
     8281 ttaagccgtg aaggagtgaa agatttccac ttctatacgc ttaaccgcgc tgaaatgagt
     8341 tacgcgattt gccatacgct gggtgttcga cctggtttat aaatagtgtg gcttttgtga
     8401 aaatcacaca gtgatcacaa attttaaaca gagcacaaaa tgctgcctcg aaatgagggc
     8461 gggaaaataa ggttgtcagc cttgttttct ccctcattac ttgaaggata tgaagctaaa
     8521 accctttttt ataaagcatt tgtccgaatt cggacataat caaaaaagct taattacgat
     8581 caatttgacc tacatctctt taaccaacaa tatgtaagat ctcaactatc gcatccgtgg
     8641 attaattcaa ttataacttc tctctaacgc tgtgtatcgt aacggtaaca ctgtagaggg
     8701 gagcacattg atgagcacgt cagacgatat ccataacacg acagccactg gcaaatgccc
     8761 gttccatcag ggcggtcacg accagagcgc gggggcgggc acaaccactc gcgactggtg
     8821 gccaaatcaa ctccgtgttg acctgttaaa ccaacattct aatcgttcta acccgctggg
     8881 tgaggacttt gactaccgca aagaattcag caaattagat tactacggcc tgaaaaaaga
     8941 tctgaaagcc ctgctgacag aatctcagcc gtggtggcca gccgactggg gcagctacgc
     9001 cggtctgttt attcgtatgg cctggcacgg cgcaggtact taccgttcaa tcgatggacg
     9061 cggcggcgcg ggtcgtggtc agcaacgttt tgcaccgctg aactcctggc cggataacgt
     9121 aagcctcgat aaagcgcgtc gcctgttgtg gccaatcaaa cagaaatatg gtcagaaaat
     9181 ctcctgggcc gacctgttta tcctcgcagg taacgtggcg ctggaaaact ccggcttccg
     9241 taccttcggt tttggtgccg gtcgtgaaga cgtctgggaa ccggatctgg atgttaactg
     9301 gggtgatgaa aaagcctggc tgactcaccg tcatccggaa gcgctggcga aagcaccgct
     9361 gggtgcaacc gagatgggcc tgatttacgt taacccggaa ggcccggatc acagcggcga
     9421 accgctttct gcggcagcag ctatccgcgc gaccttcggc aacatgggca tgaacgacga
     9481 agaaaccgtg gcgctgattg cgggtggtca tacgctgggt aaaacccacg gtgccggtcc
     9541 gacatcaaac gtaggtcctg atccagaagc tgcaccgatt gaagaacaag gtttaggctg
     9601 ggcgagcact tacggcagcg gcgttggcgc agatgccatt acctctggtc tggaagttgt
     9661 ctggacccag acgccgaccc agtggagcaa ctatttcttc gaaaacctgt tcaaatatga
     9721 gtgggtacag acccgcagtc cggctggcgc aatccagttc gaagcggtag acgcaccgga
     9781 aattatcccg gatccattcg atccgtcgaa gaaacgtaaa ccgacgatgc tggtgaccga
     9841 cctgacgctg cgttttgatc ctgagttcga gaagatctct cgtcgtttcc tcaacgatcc
     9901 gcaggcgttc aacgaagcct ttgcccgtgc ctggttcaaa ctgacgcaca gggatatggg
     9961 gccgaaatct cgctacatcg ggccggaagt gccgaaagaa gatctgatct ggcaagatcc
    10021 gctgccgcag ccgatctaca acccgaccga gcaggacatt atcgatctga aattcgcgat
    10081 tgcggattct ggtctgtctg ttagtgagcg ggtatcggtg gcctgggcat ctgcttctac
    10141 cttccgtggt ggcgacaaac gcggtggtgc caacggtgcg cgtctggcat taatgccgca
    10201 gcgcgactgg gatgtgaacg ccgcagccgt tcgtgctctg cctgttctgg agaaaatcca
    10261 gaaagagtct ggtaaagcct cgctggcgga tatcatcgtg ctggctggtg tggttggtgt
    10321 tgagaaagcc gcaagcgccg caggtttgag cattcatgta ccgtttgcgc cgggccgcgt
    10381 tgatgcgcgt caggatcaga ctgacattga gatgttcgag ctgctggagc caattgctga
    10441 cggtttccgt aactatcgcg ctcgtctgga tgtttccacc accgagtcac tgctgatcga
    10501 caaagcccag caactgaccc tgaccgcgcc ggaaatgacc gcgctggtgg gcgggatgcg
    10561 cgtactgggt gccaacttcg atggcagcaa aaacggcgtc ttcactgacc gcgttggcgt
    10621 attgagcaat gacttcttcg tgaacttgct ggatatgcgt tacgagtgga aagcgaccga
    10681 cgaatcgaaa gagctgtttg aaggccgtga ccgcgaaacc ggcgaagtga aatacactgc
    10741 cagccgtgcg gatctggtat ttggttctaa ctctgtcctg cgtgcggtgg ctgaagttta
    10801 cgccagcagc gatgctcacg agaagtttgt taaagacttc gtggcggcat gggtgaaagt
    10861 gatgaacctc gaccgtttcg acctgctgta atctgacctt cttcagcgac tgcctttcag
    10921 gcagtcgctg aagtttcttt accggcgtat agtgtccaca ggaaaactac acactggatc
    10981 tctcatgtct gccgcaggaa agagcaaccc actggcaatc agtggcctgg ttgtgctcac
    11041 acttatctgg agttatagct ggattttcat gaagcaagtc accagctaca tcggtgcctt
    11101 cgactttacc gccttacgct gcattttcgg cgctctcgtt ttattcatcg tccttttatt
    11161 acgtggtcgc ggaatgcgcc cgacaccgtt taaatacacc ttagccattg ccctgttaca
    11221 aacctgcggg atggttggcc tggcgcagtg ggcgctggtc agcggcggag cggggaaggt
    11281 ggcgatccta agctatacca tgccgttctg ggtggtgatt ttcgccgcgt tgtttctcgg
    11341 tgaacgcctg cgacgtgggc aatatttcgc gatactgatt gccgctttcg gcttattttt
    11401 ggtgttgcag ccgtggcaac tcgatttctc ttcgatgaaa agtgccatgc tggcaatcct
    11461 ctccggcgtc agttgggggg cgagcgcgat tgttgctaaa cgtctgtatg cccgtcatcc
    11521 gcgcgtggat ttattgtcgt taacatcctg gcagatgctc tatgcggcgc tggtgatgag
    11581 tgtggtcgct ttactggtgc cgcaacgtga aattgactgg cagcccaccg tgttctgggc
    11641 gctggcctac agtgcgattc tggcgacggc actggcgtgg agcttatggt tgtttgtatt
    11701 gaaaaacttg cctgccagta ttgccagctt aagcacactg gccgttcccg tttgcggcgt
    11761 actcttttcc tggtggctgc tcggcgagaa tccgggggcc gttgaaggta gcggtattgt
    11821 gctgattgtg atggcactgg cgctggtgag ccgtaagaaa aaagaagccg ttagtgtaaa
    11881 aaggatctga attttttctt catgtggggc gatcgcttat ttaacaaaat aacgataatg
    11941 ccccaccatg cgccagctaa acagcacatc ttcttcctgc gcacctgcgc caatgttatg
    12001 tatcaccagc ggcgtaccgt cgcgggcgaa gccatctgaa accaccccaa tatgtgccag
    12061 cccgttatct aatcgccagg agacaatatc gcccgcttga tagtcactgg ggttcttgct
    12121 gatggggcgt gttttatcgt ggtgggtaaa ccaggtttcc agattaggca cccgacggtg
    12181 atcgatgttg ctgtccgggc gctttaactg ccatttttgc gggtactcgg caaaattctt
    12241 cgccatatct tcgtgcacca gtttctgcaa atcgaccttc tggctgcgca atgcacggat
    12301 caccacatcg gaacataccc cgcgtttttg cgggacatca ccgccaggat aggtaagctg
    12361 catatacgcc ggatcgtaaa atagcgtgct gccgatttgc tgtctggcac cgtctgcgat
    12421 ggcaaggttg gtattggcct ggatttgtac cacggttggt ggaacggcgg gagattttaa
    12481 ggagtggctg gtaaatgccg ttagcaggct gagcagcgcc agtgaagctt tcatctggtt
    12541 cgtccgtgaa aataagagtc acagattcga ccttccggga taacgattgt tctgtagaga
    12601 aaatgtccag aagcggataa aaaactcctg aacaacgtgg gagtttcaga ttactggcac
    12661 agagtgccgg atgcggcgtg aacgccttat ctgtcctaaa aaaaaagaaa attcaatata
    12721 ttgcagagat tgcgtaggcc tgataagcgt agcgcatcag gcaattttgc gttcaaactc
    12781 ccggacaagc cgggagtttg gagtaggtta ttcccactct tgcaggaaac gctgaccgta
    12841 ctggtcggct accagcagag cggcgtaaac ctgatctggc gtcgcgccgc caggcatgtt
    12901 gtggatggtt tcacattctg cacatgccgc ttcagccaca attcgcattt tcgccgggac
    12961 atcttcttta atatccagtt gagcgagagt tattggcaaa cctaccgcat ggctaagtgc
    13021 agctacggtt tcgatttcct caaccggtgc gttttccaga accagctgcg tcagcgtacc
    13081 aaatgccact ttttcaccgt gataatagtg atgcgcgtcc gggatagcgg tcaggccgtt
    13141 atgcactgcg tgcgccgcag ccagaccacc actttcaaaa ccaacaccgc tcaaataggt
    13201 gttcgcttca atcacgcgct ccagcgccgg agtcactaca tgctgttcgg cagcaagcat
    13261 cgctttttcg ccttcttcca gcagggtgtt gtagcacagt tcagccagtg ccagcgcagc
    13321 ctgggtgcac ttgccgcccg ccatggtggt cgcgccgcta cgagagcagg cacgcgcttc
    13381 aaaccaggtt gccagcgcat cgccgatacc cgccgctaac agacgtgcag gtgcgccagc
    13441 gacgattttg gtgtcgacaa tgaccatatt cgggttattt ggcaacagca gatagcggtc
    13501 aaactcaccc tcatcggtgt agataacaga caatgcgctg cacggtgcat cggtagaggc
    13561 gatagtcggt gcgatcgcta ccggaacacc catgaaatgt gccagtgctt tggctgtatc
    13621 gagggttttt ccgccaccga taccgagaat tgcgccacac tgcgcagtct ccgcgatgcc
    13681 acgcagacgg tcgatctcat tttgcgaaca tccaccgcca aacggcgcaa tttctactac
    13741 cagtccagca tctttaaagc ttttctcgac agtggattga gcaaaaccta aaacaaattt
    13801 gtcacccacc actaaccagc gttctgccag cggcttcagg tattcgccca gacgattaat
    13861 cacatcagcg ccctggatgt atttacccgg tgattgaata atgcggtcca taattgctcc
    13921 tttagagatg agtagtgcca aatgcggcat tccagtcgtg ttcgaacttc tctatagctg
    13981 actctaccgc aggggtgttg agcatttgtt gcgctacatc taagggcagg gtgatggatt
    14041 cacatcctgc cagtaaacag tccagcgcct gacgcggcgt tttaaagctg gctgccagca
    14101 ccatgctttc tggcgcgtgc atttctaaca gcgcttgcag ctcctgaacc gtacgaatgc
    14161 cgtctccgcc cagggcatct acgcggttaa catacggagc aacgtatttt gcccctgcca
    14221 gcgcggccag taacccttgt gcagcactgt acactgcggt gccaagcgta gtaataccct
    14281 cttttttcag tattttaatt gctgccagac cttcggaagt caccgggatt ttcaccacaa
    14341 tacccggaat agcgtcgcgc aggcgcttcg cttcttccac catcccctgc gcgtcgcggc
    14401 tcatggtctg agcaaacaga atgccctcat caccaatcgc tttttgcaga cgcggcagca
    14461 cttcccatat ggactccttg ctggcagcga taatgctcgg gttagttgtc accccggcaa
    14521 tggggaatat gcgtgccaga cgttcgactt ctgcgacgtt agcggtgtcc agatacagtt
    14581 ccatgatgtt tccttttaca gttccagttc atgttgcagc aggctggcga tagcgtcggc
    14641 agaggcggcg ttaaccagcg cgttacggaa ttcttcgtgc atgatgcggc gagcgaggcg
    14701 cgagaaaatg cgcatgtgct gatcgcccgc agcgtgtttg ttcagggtta acatgatgat
    14761 gaattgcgct tcatcatcac cccagcgcac cggcgcttgc agacgcgcaa cgctgatggt
    14821 ggattgctca atgtgttctg atttgctgtg tggaatggca aaactaaagc ccagaccggt
    14881 agagaaaacg gcctcgcgcg cccacaagtc ggcttccagt ttacgcggat agcggcagcg
    14941 ccctgccagc agcaggttat cggtcatgcc tttgagcact tcttctttgc tgcgccagtc
    15001 gctttccagt gtgatgcact cggcggtgac cagcggtgcg tcttgttggg tcatgcggaa
    15061 ttgcgccagc aggtgctcca cttccagcga agtacggcag gccattgcct ggttgagcaa
    15121 cttgcggcac tcacggctat caagttgcgc catccgcgct ttcgccgccg gaattgatgg
    15181 tgcgctcatg ctgagttcat ccagccctaa gccgaccagc aacggcagca cggaaccttt
    15241 cgctcccagc tcaccgcaca gaccaatcca tttaccctgg cgatgcaccg cctgcacggc
    15301 gtaatcgagc gcccacaaga atgccggatt caggctgttg tagtgacgag taaccttagc
    15361 gttatcgcga tccactgcca gcagatattg cgtcaggtca ttactaccaa tactaaagaa
    15421 atcaatctct tcgcagcatt gatcgatgat gaacatcacc gacggcactt ccagcataat
    15481 gccgagctgg atcttctcat caaacggaat gtgttcgtta cgtagttgct gtttggcttc
    15541 tgccagtttt tctttcaccc ataagatctc ttccattgag gagatcatcg ggatcatgat
    15601 tttcaggctg ccgtgagcgg aggcgcgcag gatcgagcgt agttgtgtgg taaataacga
    15661 cgcgtattct tcataaatac gcacggcgcg ataaccgagg aacgggtttg cctctgcggg
    15721 aatgtttaga taatcaacgg gtttgtcgcc gccaatgtcc atagtgcgca caataatgct
    15781 gcgtccgttg gcagactcca gcgcctgaca aaaaatgttg tacagctcgc tttcgcccgg
    15841 tgcactggtg cgatccatat agagcatttc agtgcggaac aaaccaacgc cttccgcacc
    15901 attgccgaat gcggcctgcg cttccacgga gtgagcgatg ttagcggcaa tttcaatgcg
    15961 gataccgtca gcggtacggg cttgttgggt cagccagaca cgctgttgct cacgcagggc
    16021 gtcctgtacg cgggcttctt gctgataata acgagctacg gcttctcctg gctcaaccac
    16081 aatcgccccg gcgttgccgt cgatataaat cgtttgatgc tgccacggag tgagggcatc
    16141 aatatccaca ccaaccagcg tcggaatgtt gaacgaacgg gcaaggatca ccgtatgtga
    16201 ggtggtgccg ccacttttca gcaacagacc tttgaggtga tttttatcca gttcgaggaa
    16261 ctggctgggg gtcagttcat cagccataca aatggcggac tgcgtcagtt tgcccggtgc
    16321 cgggaagcgt tgctcaccgt agatttgctg gagtaactgg aagcagacgt cgcgtacgtc
    16381 cagggcacgt tcttgcaggt agctgctgct ggaacgggcg aactcttcgc aaaagtgatt
    16441 cgcactggcg acaatcgctt cggcgcagct taatccctcg ctgacgcctg ccagtaagtg
    16501 ctgacgtaaa gatgtatcgc cagccaggga tcggtgagct tccagtatcg cgctggtagc
    16561 accgtcgcta tccagcagac gaaactcaat gtttttcagt accagcgtca ggccgttttc
    16621 cagtgtggat tgctcggcgt caacgtcttt ggctgcggga agattactca gcgcattgag
    16681 atctaaagaa gagatcggcg tcagaatgcc gcctgcacta ccgctgcaca cggtgcgggc
    16741 gcggataatt tgcggattca gattggtcag tgaaaccggc agtggttcca gttcgtcaga
    16801 tttaacttcc gccagcggcg catcgcagtg ggggaattca tcgcgcagcc attggcttaa
    16861 acgctggtgc gcttcctgtt cgtcggcccc ggaaatcagt aactggcagt tatcgcccgc
    16921 cagcgtatcg gtgccaatca gcgcaagggc gcttttggcg ttgcccttgc ggtcagtgtg
    16981 caggttatgc cactcaattt gtgatgaaaa agtattacac agcgtttcaa cgtggcttgc
    17041 cggacgcgca tgtacgccgt taggtagctc acaaataaat tccacaatca gggccattgc
    17101 ctctctcccg gtacggtttt ctgtttacag gataagagcg cacggcaacg gcctgccatg
    17161 tgacaaatct gccaaaagct ggacaaatgt aatgtaaccg tcaatttgcg acgcgtctca
    17221 caagacgccg ttttgcggca tgcttccggt ttatcgcaag ttatgaggcg gatcgcattt
    17281 ttgtactgat attacaaaag tccagtaaat ggccttttta tccactgttt gctccgcctg
    17341 cgattgccta ttgtttcctc catcaaaaat atggacatgg gctttcgcaa gcccgggagc
    17401 agcttatgaa tgagttggtg cagatcctga aaaatacccg tcagcattta atgacgggcg
    17461 tttcacacat gattcccttc gtggtatcgg gcggtatttt gctggcggtt tccgtcatgt
    17521 tgtatggcaa aggcgcagtg ccggatgccg tagccgatcc aaatctgaaa aaactgtttg
    17581 atatcggcgt tgcgggcttg acgctgatgg tgcctttcct cgccgcttac attggttatt
    17641 ccattgcaga acgttctgcg ctggctccat gcgctatcgg tacctgggtt ggtaacagct
    17701 ttggtgcggg cttctttggt gcactgatcg ccgggattat cggcggcatc gtggtgcatt
    17761 acctgaagaa aattccggtg cataaagttc tgcgctcggt gatgcctatc ttcattattc
    17821 ctatcgtcgg cacactgatt accgcaggca tcatgatgtg gggcttgggc gagcctgtag
    17881 gggcgttgac caacagcctg actcagtggc ttcaggggat gcagcagggc agcattgtta
    17941 tgctggcggt gatcatgggt ctgatgctgg cgttcgatat gggcggtccg gttaacaaag
    18001 tggcctatgc cttcatgctg atttgcgttg ctcagggtgt ttataccgtg gtggccatcg
    18061 ctgccgtggg tatttgtatc ccgccgctgg ggatggggct ggcgacgctg attggtcgta
    18121 aaaatttctc cgcagaagag cgcgaaaccg gcaaagcggc actggtgatg ggctgtgttg
    18181 gcgttactga aggggcgatt cctttcgccg ctgccgatcc gctgcgtgtc attccttcca
    18241 tcatggtcgg ttcagtttgt ggtgcagtaa ctgcggcgct ggtcggtgcg cagtgctatg
    18301 caggctgggg tggtctgatt gtgctgccgg tggttgaagg caagctgggt tatatcgcag
    18361 cagtggctgt cggagcagtg gtgacggctg tttgtgtgaa cgtgctgaaa agtctggcgc
    18421 gtaaaaatgg gtcttcgact gatgaaaaag aagacgacct ggatttggat tttgaaatta
    18481 attaattgag gaacgagcca tgacgaaaat tattgcagta accgcatgtc cttcaggtgt
    18541 tgcccatacc tatatggcag cagaggcgct ggaaagtgcc gcgaaagcga aaggctggga
    18601 agtgaaagta gaaacctagg ggtcaattgg tctggaaaat gaactgactg cggaagatgt
    18661 ggcgagcgct gatatggtga ttctgaccaa agatatcggc atcaagtttg aagagcgttt
    18721 tgctggcaag accatcgtgc gcgtcaacat cagcgatgca gtaaaacgtg ctgacgccat
    18781 tatgagcaaa attgaagctc acctggcgca aaccgcttaa gttcttctcc cgctcgcaag
    18841 ggcgggttcg ctttcccaca ggagttcctc atgacgaatc gtatctctcg cctcaaaact
    18901 gcactgtttg ccaatacccg tgaaatctcg ctggagcggg cgctgcttta taccgccagc
    18961 catcggcaaa ccgaaggcga accggtgata ttgcgccggg cgaaagcaac agcgtatatc
    19021 cttgaacatg ttgaaatttc gattcgtgat gaagaactga ttgccggtaa ccgcaccgta
    19081 aaaccgcgcg ccgggattat gtcgccggaa atggaccctt actggctgct gaaagagctg
    19141 gatcaattcc cgacgcgtcc gcaggaccgc tttgctatca gcgaagaaga taaacgtatc
    19201 taccgtgaag agttgttccc gtactgggaa aaacgttcga tgaaagattt catcaacggg
    19261 cagatgacag atgaagtaaa agccgcgacc aacacgcaga ttttcagcat caaccagacg
    19321 gataaaggcc aggggcacat tattattgat tacccacgcc tgttgaatca cggtctgggt
    19381 gagctggtgg cacagatgca gcaacattgt cagcaacagc cggagaatca cttttatcag
    19441 gccgcgttgt tactgctgga agcctcgcag aaacacattt tgcgttacgc cgaactggcg
    19501 gaaacgatgg cggcaaactg cacagatgca cagcgtcgcg aagaactgct gactattgcg
    19561 gagatctctc gccataacgc cgaacataag ccgcagacgt tctggcaggc gtgccagtta
    19621 ttctggtaca tgaacatcat tctgcaatac gaatccaacg ccagttcgct gtctttaggg
    19681 cgcttcgacc agtatatgtt gccgttctat caggcatctt taacccaggg cgaagatccg
    19741 gcgttcctga aagaactgct cgaatcttta tgggtgaaat gcaacgacat cgtgctgttg
    19801 cgctccacca gcagcgcgcg ttatttcgca ggtttcccga ccggctatac cgcactgctc
    19861 ggcgggttaa ccgagaacgg acgtagcgcg gtgaacgtgc tttcgttcct ttgccttgac
    19921 gcctatcaaa gcgtgcaatt accgcaaccg aacctcggcg tgcgcactaa cgccttgatc
    19981 gacacgccgt tcctgatgaa aaccgccgaa accattcgcc tcggcaccgg tattccgcaa
    20041 atctttaacg atgaagtggt ggtaccagcg ttcctcaacc gtggcgtttc gctggaagat
    20101 gcgcgcgact attccgtagt gggctgtgtg gaattatcta ttcccggcag aacctacggc
    20161 ttgcatgaca tcgcgatgtt taatctgctg aaagtgatgg aaatctgcct gcatgaaaat
    20221 gaaggcaatg ctgcgctgac ttatgaaggt ttactggagc agatccgcgc caagatcagc
    20281 cactacatca ccctgatggt tgagggcagc aatatttgtg atatcggcca tcgcgactgg
    20341 gcacctgtac cgctgctctc gtcttttatc agcgattgtc tggaaaaagg tcgcgatatt
    20401 accgatggcg gcgcgcgtta taacttctcc ggcgtacagg ggatcggtat cgccaacctg
    20461 agcgattctc tccatgcgtt gaaagggatg gtttttgagc aacagcgttt aagttttgac
    20521 gaattgctgt cggtattaaa agccaacttt gcaacgccag aaggcgaaaa agtccgtgct
    20581 cgcttaatta accgcttcga gaaatacggt aacgatatcg acgaggtgga taacatcagc
    20641 gccgaactgt tacgccacta ctgcaaagaa gtggaaaaat accagaaccc gcgcgcggtt
    20701 acttcacgcc gggatcgtat accgtttctg ctcacgtccc gttgggatcg gtggttggcg
    20761 cgacgccaga cggtcgtttt gccggggaac aactggcaga cggcggcttg tcacctatgc
    20821 tgggccagga cgcacaaggg ccaacagcgg tactgaagtc agtcagtaag ctcgataata
    20881 cgctgctgtc taacggtacg ttgctgaacg tgaaattcac tccggcgacc ctggaaggtg
    20941 aagcgggatt acgcaaactg gccgacttct tacgggcgtt tacccagctt aagttgcagc
    21001 atattcagtt taacgtggtg aacgccgaca cgttgcggga agcgcaacag cgcccacaag
    21061 attatgccgg gctggtggtg cgcgttgccg gatacagcgc cttctttgtc gaactgtcga
    21121 aggagatcca ggatgacatc atccgccgga cagcgcatca gctgtaacgt tgtggaaacg
    21181 cgccgcgatg atgtggcgcg cattttcaac attcagcgtt attcactgaa tgacggcgag
    21241 ggcattcgta cggtggtctt ttttaaaggc tgtccgcatc tttgcccgtg gtgtgctaat
    21301 ccggagtcga tctccggcaa aatccagacg gtacgcagag aggcgaaatg tctgcactgt
    21361 gcgaaatgtt tgcgtgatgc ggatgaatgc ccctccgggg cgtttgaacg gattggtcgc
    21421 gatatcagcc ttgacgctct ggaacgggaa gtgatgaaag atgacatttt ctttcgcacg
    21481 tccggcggcg gcgtcacgct ttctggcggc gaagtgttaa tgcaggcgga gtttgctacc
    21541 cgttttttac agcgactgcg gctgtggggt gtgtcatgcg ccattgaaac tgccggagac
    21601 gcgccagcaa gcaagctgtt accgctggcg aaattgtgcg atgaagtgct gttcgattta
    21661 aaaattatgg acgcgactca ggcgcgggat gtggtgaaga tgaacctgcc acgcgtgctg
    21721 gagaatctgc gtttgctggt gagtgagggt gtcaacgtga tcccgcgttt accgttgatc
    21781 cctggtttca cgctcagcag ggagaatatg cagcaggcgc tggatgtact gatcccgctg
    21841 aaaatcaggc agatccatct gttaccgttt catcagtacg gcgaaccgaa ataccgcctg
    21901 ctggggaaaa catggtcgat gaaagaggtg cctgcgccgt cgtcagccga tgtgacaacg
    21961 atgcgcgaaa tggcagaacg ggccggattt caggttaccg tgggaggtta aaatggcata
    22021 cctggtggca gtaaccgcct gcgtaagcgg cgtggcgcat acttatatgg cggcggaacg
    22081 gctggaaaag ttgtgccagt tagagaagtg gggcgtcagc attgaaactc agggcgcgct
    22141 gggaacggag aatcgtttag cggacgagga tatccgtcgg gcggatgttg ctctgttgat
    22201 tacggatatc gagcttgccg gtgccgagcg atttgaacat tgccgctatg tgcaatgcag
    22261 catctacgca ttcctgcgtg agccgcagcg ggtaatgagc gcggtgcgca aagtgctttc
    22321 tgcgccgcag caaacccatc ttattctgga gtagtcggtt tttctgtcag ttggctgtgg
    22381 tactgccggc gatattccga cggcgagcgt tcggtgtttt tacgaaacag acggcagaag
    22441 tagttgctgt cgacaaagcc gcaggcgtgc gccacttctt ttaccttcag gtcatagcct
    22501 ttcaataacg tcttagcgtg ctccagtcgc gtgtgattca ggtattcgtt aaaaccaatg
    22561 gcccccgttt tttgaaacag gtgcgagaga tagtttggtg aaatatagaa tgcctgtgcg
    22621 acagattcgc gggtaagcgc ggaggcatag cgttcgtcga tataatcgcg aatagcttca
    22681 aatagtgcct ggctgcgtga ggcggtctgg atttggctgc cgagcaaatc gcggcagtgg
    22741 ctaagcaggc tggctacgat aagccttgcc gtttgctgct cctgcggctg catttgcatt
    22801 tcattgagcg tttgtagcag aaaagaacct atacgtgggc cgcgtcgggc gacgtgttgc
    22861 ttcgccagat tttgatattg tttgccatcc cattgcacga cgctgaagcc gagttgctgt
    22921 ttgccaaaca gcacgctaaa ggtggtagcg ggtgttttcc attgtgggaa attccagcca
    22981 ccagcggcaa cgtacagcac atcgccggga actaacgttg ctccagcgcc ggtatcaaca
    23041 aactcacctt ccagcacaat ttccagccgt ggaaaatcga cctgatacgc aagatcgggc
    23101 accgggccgt tactgctggc aaagtaaatc tggcgcaggg acagcgggcc gttgatcagg
    23161 cgggagagca gatagctgac gccgtgatac atgttattcc cttaggtatg tagacgggaa
    23221 aacagtaact ggcattatta aaaaaggcca cctgaaaagt gtggcctgag gggagttcga
    23281 tgtaaacatt gcggcagttg tattgccgga tgtggcgtaa acgccttatc cagcctaccg
    23341 ccgcgatctg taggccggat aagacgcgtc aagcgtcgca tccggcaaat agcgcctggc
    23401 tgataattac tgattaccta cctggtcgcc atacggcaga gtgtcgtaat cgatcagcgc
    23461 gtttttcttg tacgggttac caatccagcg ggtagtttct ttgaactgcg gattcaccac
    23521 tgaacgggtt ggatcgtaac cgtcgtaaga taagcccgcc aaatctgacc aggtgtggat
    23581 cagttccgcc aggctgtatt tacgatcgac atcctgtgag aaatcacggg gatgagtcgc
    23641 ttgccatttt tccgacgtcc acagcaggaa cggaatggtg tacatgtggc gcgtcgggtt
    23701 gtcttcgtta cgcccctgag ttttgtgcgg cggcgtgtcg taaacctctt caccgtggtc
    23761 agagaagtaa accaggaatc cgttcgggtc tgccgcttta aagtctttaa tcaggctggc
    23821 aaccacatga tcgttataca ggttagcgtt gtcataatcg ttatatgatt ccagctcttc
    23881 tgcgcttaat cctggcggaa catgatcggt attgccatca aacttgccct gattttccgg
    23941 gtagcggtat ttgtatttga tatgcgtacc cagcagatga acgatgatca gtttcttcgg
    24001 cgcagggtca ttcagcacat cctggaacgg cttcagcacg ttggtgtcgt attcacgcgc
    24061 actctgcgta cgttgctggt tcatgtagta ctgcttgtcg gtctggcgcg aaaataccgt
    24121 cagcatggta ttgcgggcgg tcatcgtctg ctggttggtg atccagaagg ttttataacc
    24181 cgcctgtttc atcatgttca tcagcgacgg ctgcgtcaga tacagatccg ggttcttttc
    24241 attggcaaag gtcagcgcct gttgcaggat ttcaatggtg tacggacgag acgtaactac
    24301 gttattaaac acggtcagat tcggatcggt tttatgcagc gcatccagct ccggcgtggt
    24361 ttcacgcgga taaccgtaca gactcatgcg tccgcgctgg gtcgactcgc caatcaccag
    24421 cactaaagtg cgcggttcgt tacccgattc atctttgaaa ttagccagtg gcggcaaggc
    24481 attattttca ttcagtaact ttgttagcga gtttagttgc tgacgatact gataatagcc
    24541 ggtcaggaat tgccacggtg cggcaggctc catacgcgag gccaggttat ccaacgtttt
    24601 ctcaaacggc ttgtttttga taaacgtatt catggcgatc ggatgcagaa tcaagccata
    24661 aagcagggca aaagagacaa cataacgcca cggctttgga atatagaccg ggcgcaggcg
    24721 tgtccacagc agaactgcca ccgccgtata ggccagcgcg ataagcacaa ttttcaggct
    24781 gaaatactgg cttaaatact cgctggcttc gttggtgttg gtttcgaaca tcacaaacag
    24841 aacgctctgc gagaactcct gcccgtagat gacgtagtag catagcgccg ccagagaggc
    24901 cgcccatagc accacgccga ttactgcggc aataatttta atccgcttcg gaaagaggaa
    24961 taccgggatc aaccacagcg agctgaataa cagcgagtcg cgaatgccgt tggtgccact
    25021 ataaccactg atgtaaataa tggcctgtag cagagtagag aaaaaccaaa agtagagcag
    25081 tgcccaaccc agggctttcc agctaaaaag gggtttagcc tggacttctg tggaatgcat
    25141 agtaagaacc tgtcttgaaa aaaatatcgc cgaatgtaac gacaattcct taaggatatc
    25201 tgaaggtata ttcagaattt gaataaaatg cagacagaaa tatattgaaa acgagggtgt
    25261 tagaacagaa gtatttcaga aaaccctcgc gcaaaagcac gagggtttgc agaagaggaa
    25321 gattagccgg tattacgcat acctgccgca atcccggcaa tagtgaccat taacgcttgt
    25381 tcgacgcgag gatccggttc ctggccttct ttttctgcct ggcgggagcg gtgcagcaac
    25441 tcggcctgca atacgttcag cgggtcggtg taaatattcc gtagctgaat agactctgca
    25501 atccacggca gatcggccat cagatgggaa tcgttggcaa tcgccagcac cactttgatg
    25561 tcttcttctt gcaggttgcg taactcttta cctaacggcc acagtgcttt gtctaccaga
    25621 cgttgatcat agtattccgc caaccacagg tctgctttgg cgaagaccat ctccagcatg
    25681 ccgagacgcg tcgagaagaa tggccaatcg cggcacatgg cttccagctc gctctgtttg
    25741 ccatcttcga ccactttttg cagcgccgta cctgcaccca gccaggcggg gagcatcagg
    25801 cggttttgcg tccaggcaaa aatccacggt atggcgcgca gcgactcgac gccgccggtt
    25861 gggcgacgtt tcgccggacg tgaacccaac ggcagtttgc ccaattcttg ttccggcgta
    25921 gcggagcgga agtaaggcac aaaatctttg ttttcacgta cgtagccacg gtagagatcg
    25981 caggagatga ctgacagttc atccataatg cgacgccagc tctctttcgg ctcaggtggt
    26041 ggcagcaggt tggcttccag aatcgccccg gtgtaaagcg acaggctgct gacggtgatt
    26101 tctggcagac catatttaaa gcggatcatc tcgccctgtt cagtcacgcg caggccacct
    26161 ttcaggcttc ctggcggttg tgacagcagc gccgcatggg caggtgcgcc gccgcgacca
    26221 atggaaccgc cgcgaccgtg gaacaacgtc agctcaatac ccgctttttc gcaggttttg
    26281 attaatgcat cctgtgcctg atattgcgcc caggaagctg ccatcacgcc cgcatctttt
    26341 gctgagtcgg aatagccaat catcaccatc tgtttgccct gaatcaggcc gcgataccag
    26401 tcgatattga gcagctgggt catgacatcg ttggcgttgt tcaggtcatc gagggtttca
    26461 aacagcggag caaccggcat cgcaaacccg atacccgctt ctttcagcag caggtggaca
    26521 gccagtacgt cggacggcgt tttcgccatc gagatcacgt aggcggcaat ggagccttgc
    26581 ggcgcttcgg caatcacctg gcaggtatcg agcacttcgc gcgtttcggc gcttggttgc
    26641 cagttgcgcg gcagaagcgg acgtttggag ttcagttcgc ggatcaggaa cgcctgtttg
    26701 tcggcctctg accagctttc gtagtcgccg ataccgaggt agcgggtcag ctcgcccagc
    26761 gcttcggtat ggcgggtgct ctcctgacgg atatcaatac ggaccagcgg tacgccgaaa
    26821 catttcacgc ggcgcagggt gtcgagcaga tcgccgttgg cgataatacc catgccacac
    26881 gcctgaagtg actggtagca agcgtagagc ggttcccaca gttcttcgtt ttgtgtcagc
    26941 agcccttctg gttttggcag ttcttcgcct ttcaggcgcg cttccagcca tgcctgtgtc
    27001 gccatcaggc gagaacgcag gtttttcatc agatagcgat acggttctgc ggcaccttct
    27061 tcgccaacca gcgccagcag ttcaggggtc gcttcaacca tcgacagttc agaaaccaga
    27121 acctgaatat ctttcaggaa cagatcggtg gctttccagc gactgagtag caggacgtgg
    27181 cgggtgatat cggcagtgac gttcgggttg ccgtcgcggt caccgcccat ccacgaagta
    27241 aaacggaccg gaacaaattc gacgggcagt ttgtagccga ggttctcttc cagttgttcg
    27301 ttcagttcgc gcaagtaatt cggtacgcct tgccacaggc tgttttctac tacggcaaag
    27361 ccccatttgg cttcatctac cgggcttgga cgcagcttac ggatttcatc agtatgccat
    27421 gactgggcga ttaactggcg caggcgacgc atcagctggt tgtgttcgta gtcagcgata
    27481 tctttgttat cgagctgttt taagcaggcg ttcacttcca ccattttgtg gatcagtgta
    27541 cgacgggtaa tttcggttgg gtgagccgtg aggaccagtt ccagcgacag cgattccact
    27601 gcttttttga tggtgtcttc gctcagttcc ggctggtttt tcagtttgcg cagggtgcgg
    27661 gcgatcactt ccgggttgct ggcagcttcg cctttcggcg aaatgctgtg gtattgctcg
    27721 gcggtgttgg ccaggttcag gaactgacta aacgcacgcg caacgggcag cagctcgtcg
    27781 ttcgacaaat tttgtaaggt ggtgagcaac tcctggcggt tagcatcatt gccagcgcgt
    27841 gaagatttcg acaacttacg gatagtttct acgcgttcaa gaatgtgttc tcccaacgca
    27901 tccttgatgg tttctcccag cactttgccg agcatactga cattactacg caatgcggaa
    27961 tattgttcgt tcatattacc ccagacaccc catcttatcg tttgatagcc ctgtatcctt
    28021 cacgtcgcat tggcgcgaat atgctcgggc tttgcttttc gtcgtctttt ataaagccac
    28081 gtaaaagcgg tgacgtcaaa tgctgcgaaa tcgcttcagc aaacgaataa atagcaggaa
    28141 tttacgccat taaattcacg acgctttaaa taagcgtaac ttatggaaat gttaaaaaat
    28201 cgccatacat aacaccaaag gtgtaggtcg gataagatgc gcaagcatcg catccgacat
    28261 tattgcggca ccggagtttg gcaacggtgc cggatgcggc gcgagcgcct tatccggcct
    28321 acgttttaat gccagcaaaa atggtgaatt acctggataa tcaactcacg ggtgggttta
    28381 ataaaccgcg tctccagata ttcatcaggc tgatgagctt gattgatgga acccggaccg
    28441 agcaccagcg tcgggcataa cgtttgaata aacggcgctt cggtacagta attcaccact
    28501 tcggtttttg ctccgagcaa tttctcaacc acttcaacca gttgatgatt cggtgggcat
    28561 tcatagccag ggatcggcgg atgcagctcg tcgaccgtca gacgacccgg ccagcgttcg
    28621 ctcaccggag ccaatgcatc gttgagcaaa ccattaagtt cattgagtgt catgctaggc
    28681 agcggacgaa tatccatatg caactcacag caagcgcaaa tacggttaga agcgtcgcca
    28741 ccgtgaatat gcccgaggtt gagcgtaggg tatggcacgg taaatgcttc gtagtgataa
    28801 cgttctttca ggttatcgcg caattgcaaa atatgcccga tggcgtcgtg cattagttcg
    28861 atagcgttaa ctccgcgtgc tggatcgctg gagtgccccg actggccctg aatacggatg
    28921 gcgttagaga tatgaccttt atgtgcgcgt accggttgta gtgacgtcgg ttcgccaatg
    28981 atggcgcaat ccgggcgcag ggcggtagtt tcggcaaaat aacgcgctcc ggccatactg
    29041 gtttcttcat cagcagtcgc cagaatgtag agcggttttt tcagtttcgt gacgtcgaca
    29101 tcgcgtagcg tatcaaggat aaacgcaaaa aagcctttca tgtcggcggt gcctaagccg
    29161 taaagcttgc cgtcatgctc cgtcagtgta aacggatcgc gcgtccagcg accgtcatca
    29221 aatggcaccg tatcggtatg ccccgccagc aacaagccgc cagccccctg tccgatactt
    29281 gccagcatat tgaatttgtt gcgagttcct ggcacaggct gcacttccac attgaagcct
    29341 aaatctttaa accagtccgc cagcagagtg attaaatctg cattgctttg atcgagtgcc
    29401 tcttccgtgg cgcttattga aggtgtggca atcagagcac ggtaaatctc gataaatggc
    29461 ggtaatttgt ttttcattgt tgacacacct ctggtcatga tagtatcaat attcatgcag
    29521 tatttatgaa taaaaataca ctaacgttga gcgtaataaa acccaccagc cgtaaggtga
    29581 atgttttacg tttaacctgg caaccagaca taagaaggtg aatagccccg atgttgaata
    29641 cgctgattgt gggtgccagc ggctacgctg gcgcagagct agtgacctat gtaaatcgcc
    29701 atccgcatat gaacataacc gctttgactg tttcagcgca aagcaatgat gcgggaaagt
    29761 taatctccga tttgcatccg cagctaaaag gcatcgttga gctgccgttg cagccgatgt
    29821 cggatatcag cgagtttagc ccaggggtgg acgtagtgtt tctcgccacc gcccatgaag
    29881 ttagccacga tttagcgccg cagtttcttg aagcgggctg cgtggtgttc gacctttccg
    29941 gtgcgtttcg tgttaacgac gccaccttct atgaaaaata ttacggcttt acccatcaat
    30001 acccggaact gctggaacag gcagcctacg gtctggcgga gtggtgcggt aataaattaa
    30061 aagaagcgaa tttgattgcg gtgccaggct gttatccgac ggcggcacag ctggcgctga
    30121 aaccgttgat tgatgccgat cttcttgacc tcaatcagtg gccggtgatc aacgccacca
    30181 gcggcgtgag cggtgcagga cgtaaggcag cgatttcaaa tagtttttgt gaagttagcc
    30241 tgcaaccgta tggcgtcttt actcatcgcc atcaaccaga gatcgccaca cacctcggtg
    30301 ctgacgttat cttcacccca catctgggca atttcccgcg cggcattctc gaaaccatta
    30361 cctgccgcct gaaatcgggt gtgacccagg cgcaagtcgc gcaagtgtta cagcaggcgt
    30421 atgcccataa accgctggtg cggctgtatg acaaaggcgt tccggcgctg aaaaatgtcg
    30481 ttgggctgcc attttgcgat atcgggtttg ccgttcaggg cgagcatctg attattgtgg
    30541 cgaccgaaga caacttactg aaaggcgcgg cggcacaagc ggtacagtgc gccaatattc
    30601 gtttcggcta tgcggaaacg cagtctctta tttaagggtg caatgatgaa tccattaatt
    30661 atcaaactgg gcggcgtact gctggatagt gaagaggcgc tggaacgtct gtttagcaca
    30721 ctggtgaatt atcgtgagtc acatcagcgt ccgctggtga ttgtgcacgg cggcggttgc
    30781 gtggtggatg agctgatgaa agggctgaat ctgccggtga aaaagaaaaa cggcctgcgg
    30841 gtgacgcctg ctgatcagat agacattatc accggagcac tggcgggaac ggcaaataaa
    30901 accctgttgg catgggcgaa gaaacatcag attgcggccg taggtttgtt tctcggtgac
    30961 ggcgacagcg tcaaagtgac ccagcttgat gaagagttag gtcatgttgg actggcgcag
    31021 ccaggttcgc ctaagcttat caactccttg ctggagaacg gttatctgcc ggtggtcagc
    31081 tccattggcg taacagacga agggcaactg atgaacgtca atgccgacca ggcggcaacg
    31141 gcgctggcgg caacgctggg cgcggatctg attttgctct ccgacgtcag cggcattctc
    31201 gacggcaaag ggcaacgcat tgccgaaatg accgccgcga aagcagaaca actgattgag
    31261 cagggcatta ttactgacgg catgatagtg aaagtgaacg cggcgctgga tgcggcccgc
    31321 acgctgggcc gtccggtaga tatcgcctcc tggcgtcatg cggagcagct tccggcactg
    31381 tttaacggta tgccgatggg tacgcggatt ttagcttaag ttttgttggc cggaggcgca
    31441 gctttccggc attgaatttc aaaataagga aacagagtta tggcactttg gggcgggcgt
    31501 tttacccagg cagcagatca acggttcaaa caattcaacg actcactgcg ctttgattac
    31561 cgtctggcgg agcaggatat tgttggctct gtggcctggt ccaaagccct ggtcacggta
    31621 ggcgtgttaa ccgcagaaga gcaggcgcaa ctggaagagg cgctgagcgt gctgctggaa
    31681 gatgttcgcg ccaggccaca acaaatcctt gaaagcgacg ccgaagatat ccatagctgg
    31741 gtggaaggca aactgatcga caaagtgggc cagttaggca aaaagctgca taccgggcgt
    31801 agtcgtaacg atcaggtagc gactgacctg aaactgtggt gcaaagatac cgttagcgag
    31861 ttactgacgg ctaaccgaca gctgcaatcg gcgctggtgg aaaccgcaca aaacaatcag
    31921 gacgcggtca tgccaggtta cactcacctg caacgcgccc agccggtgac gttcgcgcac
    31981 tggtgcctgg cctatgttga gatgctggcg cgtgatgaaa gccgtttgca ggatgcgctt
    32041 aagcgtctgg atgtcagccc attaggctgt ggcgcgctgg cgggaacggc ctatgaaatc
    32101 gaccgtgaac agttagcagg ctggctgggc tttgcttcgg cgacccgtaa cagtctggac
    32161 agcgtttctg accgtgacca tgtgttggaa ctgctgtcgg ctgccgctat cggcatggtg
    32221 catctgtcgc gttttgctga agatctgatt ttctttaaca ccggcgaagc ggggtttgtg
    32281 gagctttctg accgcgtgac ttccggttca tcattaatgc cgcagaagaa aaacccggat
    32341 gcgctggagc tgattcgcgg taaatgcggt cgggtgcagg gtgcgttaac cggcatgatg
    32401 atgacgctga aaggtttgcc gctggcttac aacaaagata tgcaggaaga caaagaaggt
    32461 ctgttcgacg cgctcgatac ctggctggac tgcctgcata tggcggcgct ggtgctggac
    32521 ggcattcagg tgaaacgtcc acgttgccag gaagcggctc agcagggtta cgccaacgcc
    32581 accgaactgg cggattacct ggtggcgaaa ggcgtacctt tccgcgaggc gcaccatatc
    32641 gtgggtgaag cggtggtgga agccattcgt cagggcaaac cgctggaaga tctgccgctc
    32701 gacgagttgc agaaattcag cccggtgatt ggcgaagatg tctatccgat tttgtcgctg
    32761 caatcgtgcc tcgacaagcg tgcggcaaaa ggcggcgtct caccgcagca ggtggcccaa
    32821 gcgattgctt ttgcgcaggc gcggttgggg taagaacatt tatatgtaca aatttgagcc
    32881 tggcttatcg ccgggctttt ttatggcaaa aaaaagcgga tcctggagat ccgcaaaagt
    32941 tcacgttggc tttagttatt cgagttgaga aactctcgaa acgggcagtg acttcaaggg
    33001 ttaaaagagg tgccgctccg tttctgtgag caattatcag tcagaatgct tgatagggat
    33061 aatcgttcat tgctattcta cctatcgcca tgaactatcg tggcgatgga ggatggataa
    33121 tgaatattcg tgatcttgag tacctggtgg cattggctga acaccgccat tttcggcgtg
    33181 cggcagattc ctgccacgtt agccagccga cgcttagcgg gcaaattcgt aagctggaag
    33241 atgagctggg cgtgatgttg ctggagcgga ccagccgtaa agtgttgttc acccaggcgg
    33301 gaatgctgct ggtggatcag gcgcgtaccg tgctgcgtga ggtgaaagtc cttaaagaga
    33361 tggcaagcca gcagggcgag acgatgtccg gaccgctgca tattggtttg attcccacgg
    33421 ttggaccgta cctgctaccg catattatcc cgatgctgca ccagaccttt ccaaagctgg
    33481 aaatgtatct gcatgaagca cagacccacc agttactggc gcaactggac agtggcaaac
    33541 tcgattgcgt gatcctcgcg ctggtgaaag agagcgaagc attcattgaa gtgccgttgt
    33601 ttgatgagcc aatgttgctg gctatctatg aagatcaccc gtgggcgaac cgcgaatgcg
    33661 taccgatggc cgatctggca ggggaaaaac tgctgatgct ggaagatggt cactgtttgc
    33721 gcgatcaggc aatgggtttc tgttttgaag ccggggcgga tgaagataca cacttccgcg
    33781 cgaccagcct ggagacgctg cgcaacatgg tggcggcagg tagcgggatc actttactgc
    33841 cagcgctggc tgtgccgccg gagcgcaaac gcgatggggt tgtttatctg ccgtgcatta
    33901 agccggaacc acgccgcact attggcctgg tttatcgtcc tggctcaccg ctgcgcagcc
    33961 gctatgagca gctggcagag gccatccgag caagaatgga tggctatttc gataaagtgt
    34021 taaaacaggc ggtttaaacc gtttaacgca gctacccgat aggcttccgc catggtcggg
    34081 tagttaaagg tggtgttgac gaagtactca atagtgttgc cgccaccttt ctgttccata
    34141 atcgcctgac cgatatgaat aatttcggca gcgcgctcgc caaagcagtg aatacccaga
    34201 atctcttttg tttcccgatg gaacaaaatt ttcagcgtgc ccacgttcat gccgacgatt
    34261 tgtgcgcgtg ccagatgttt aaactgggcg cggcccactt catatggcac tttcatcgcg
    34321 gtcagctgct gttcggtttt gcccacagag ctgatttccg ggatggtgta aatgccggta
    34381 gggatatctt caatcagatg cgcggtggct tcgcctttta ccagcgcctg cgcggcaatg
    34441 cgcccctggt cataggccgc tgatgccagg ctcggataac caatcacgtc gcccaccgcg
    34501 taaacgtgcg gctgtgcggt ctgatacatg ctgttgacct tcagctgtcc gcgactgtca
    34561 gtttccagcc caatgttctg taacgccagc gaatcggtat taccggtacg accgttggca
    34621 tagagcaggc agtcagcttt cagtttttta cccgacttca gatgcatgat cacaccgtcg
    34681 tcacagcctt cgatcttctc gtactcttcg ttgtgacgaa tcactacgcc actgttccag
    34741 aagtgatagg agagagaatc tgacatctct tgatcgagaa atgccagcag gcgatcgcgg
    34801 gtgttgatca gatccacttt tacatccata ccgcggaaga tcgacgcata ttcacagccg
    34861 atcactccag caccatagat aagtacatgg cgcggttcgt ggtgcatgct aagaattgag
    34921 tcgctgtcgt aaatgcgtgg atgggtgaaa tcaacatctg ttggatgata tggacgagag
    34981 ccgcaggcaa taacaaattt ttcagcggtt agtgtttcga cgctgccgtc cgggcaatcc
    35041 agcgccaacg tatgctcgtc aacaaagcga gcgttcccct gcaatatttc acagtgatta
    35101 cgttcgtaaa atccctgacg catgcgcgtt tgttgattaa tcacgttatc ggcatggtta
    35161 aggatatcgg caaaagaaga gcggagcagt cgcgaatggt cgctgtatag tgggttttga
    35221 ttgaattcta taatgcggct gacggcgtga cggagagctt tcgacgggat ggtgccccag
    35281 tgggtgcaac cgccgccaac attttgataa cgctcgataa ctgcgacgcg cacaccttgc
    35341 ttaaccaggc ccattgcagc gccttcgccg ccggggccgg aacctattac tatggcatcg
    35401 taatcgtagg aatgtggcat ggtagggctt acctgttctt atacataaaa gcaacagaat
    35461 ggtaacattc tatcgcgggt aagccaattg atccccgtca tttatctggc tatatcctga
    35521 gcggcctttg ctttgtctgt ttcttacttt tgccctgacg ttttattgga tttttatcga
    35581 cgatactctc cgtttaagcg gcaggtttcc gctgtacgta aaagaaccgg ccagtaaata
    35641 tgttttattg cgttaccgtt cattcacaat actggagcaa tccagtatgt tcattctctg
    35701 gtatagtgcc agcagtactt ttggcaagga ttcagacatc gtgatgggca taagagcgca
    35761 acaaaaagaa aaaacccgcc gttcgctggt ggaagccgca tttagccaat taagtgctga
    35821 acgcagcttc gccagcctga gtttgcgtga agtggcgcgt gaagcgggca ttgctcccac
    35881 ctctttttat cggcatttcc gcgacgtaga cgaactgggt ctgaccatgg ttgatgagag
    35941 cggtttaatg ctacgccaac tcatgcgcca ggcgcgtcag cgtatcgcca aaggcgggag
    36001 tgtgatccgc acctcggtct ccacatttat ggagttcatc ggtaataatc ctaacgcctt
    36061 ccggttatta ttgcgggaac gctccggcac ctccgctgcg tttcgtgccg ccgttgcgcg
    36121 tgaaattcag cacttcattg cggaacttgc ggactatctg gaactcgaaa accatatgcc
    36181 gcgtgcgttt actgaagcgc aagccgaagc aatggtgaca attgtcttca gtgcgggtgc
    36241 cgaggcgttg gacgtcggcg tcgaacaacg tcggcaatta gaagagcgac tggtactgca
    36301 actgcgaatg atttcgaaag gggcttatta ctggtatcgc cgtgaacaag agaaaaccgc
    36361 aattattccg ggaaatgtga aggacgagta atgaaacaag caaatcaaga tagaggtacg
    36421 ctgctgctgg cgttagttgc tggcttatcg attaatggta ctttcgcagc gctgtttagc
    36481 tccattgtgc cattttctgt attcccgatt atttccctgg tgctgacggt ttactgcctg
    36541 catcaacgtt atcttaatcg cactatgccg gtaggcttgc cgggtctggc agctgcctgt
    36601 tttattctcg gcgtactgct gtacagcacg gtagttcgtg cggaatatcc ggatatcggc
    36661 tctaacttct tcccggcagt actctccgtc attatggtgt tctggattgg cgcgaagatg
    36721 cgtaaccgta agcaggaagt tgctgagtaa tcggtattat gccggatgct ggcgcatccg
    36781 gcatgggttt tacttcgcgg tcagtaatac gccgcactcc atatggtgcg tgtaggggaa
    36841 ctgatcaaac agagccagac gttcgacctt gtgcgtctgg cttaatgttt ccagattctt
    36901 gcataacgtt tccgggttac aggagatgta caaaatacac ggatacgcct gcaccatttt
    36961 ctcggtttca ctgtccagac cgctgcgcgg tgggtcgaca aaaatggttt cgcactgata
    37021 actctttaag tcgatccctt gcaggcggtt aaactcgcgc acaccattca tcgcctgagt
    37081 aaattcttct gccgccatac gaataatttg tacgttatca atatggttag ctgcgatgtt
    37141 gtattgcgca gcagcaaccg acggcttagc gatttcggtg gctaataccc gatcaaaatt
    37201 gcgcgccagc gctaatgaaa agttaccgtt gccgcagtac agctccagta aatcgccttt
    37261 tgagcctttg gttacgtcca gcgcccattc cagcatctga atattcatcg ccgcgttcgg
    37321 ctgggtaaag ctgttttcta cctgacggta gatcatctct ttccctgcga ccggcagacg
    37381 ctcgtcgatg taatcttgat ccagctcgat tttggttttc gttgcccgac caatcagatg
    37441 cacattcaga ttctgcgcgc gcagtgcatc gcgcagggcc tccgcttcct gacgccactc
    37501 atcatccagc ttcttatggt atagcaggga aaccaccgcc tgattactca gcgtggtgag
    37561 gtaatcaatc tggaacaact tgtggcgcag aacgggatta ttacgcacac ccgcaatcat
    37621 cgccgtcatc aactggttga taagttcact ggcggcgggg aagctatcca cgcggatacg
    37681 gcttttggtt tgttgatcga aaatgatgtg atacaggtca tcgccatcgt gccagatgcg
    37741 gaactccgca cgcatccggt aatgactgac cggcgagcga aacacttccg gaaccaggtc
    37801 agaaaacggt gccatcatac tttgcaaacg taccactttt tcggctaact gcgcttcata
    37861 ctgttctgtt ggaaggtgtt cgggggtcat gatgtatcct gaaaaattaa agtacgcggc
    37921 gattgtaggg attgctcatc agatgtccag atcttgatga attcctattt gtgagctacg
    37981 tctggacagt aacttgttac aacctgtagc atccacttgt cggtcctgtg agttaatagg
    38041 gaatccagtg cgaatctgga gctgacgcgc agcggtaagg aaaggtgcga tgattgcgtt
    38101 atgcggacac tgccatccgg tgggaagtca tcatctctta gtatcttaga tacccctcca
    38161 agcccgaaga cctgccggcc aacgtcgcat ctggttctca tcatcgcgta atattgatga
    38221 aacctgcggc atccttcttc tattgtggat gctttacaat gattaaaaaa gcttcgctgc
    38281 tgacggcgtg ttctgtcaca gccttttccg cttgggcaca ggataccagc ccggatactc
    38341 tcgtcgttac tgctaaccgt tttgaacagc cgcgcagcac tgtgcttgca ccaaccaccg
    38401 ttgtgacccg tcaggatatc gaccgctggc agtcgacctc ggtcaatgat gtgctgcgcc
    38461 gtcttccggg cgtcgatatc acccaaaacg gcggttcagg tcagctctca tctattttta
    38521 ttcgcggtac aaatgccagt catgtgttgg tgttaattga tggcgtacgc ctgaatctgg
    38581 cgggggtgag tggttctgcc gaccttagcc agttccctat tgcgcttgtc cagcgtgttg
    38641 aatatatccg tgggccgcgc tccgctgttt atggttccga tgcaataggc ggggtggtga
    38701 atatcatcac gacgcgcgat gaacccggaa cggaaatttc agcagggtgg ggaagcaata
    38761 gttatcagaa ctatgatgtc tctacgcagc aacaactggg ggataagaca cggggaacgc
    38821 tgttgggcga ttatgcccat actcatggtt atgatgttgt tgcctatggt aatacaggaa
    38881 cgcaagcgca gacagataac gatggttttt taagtaaaac gctttatggc gcgctggagc
    38941 ataactttac tgatgcctgg agcggctttg tgcgcggcta tggctatgat aaccgtacca
    39001 attatgacgc gtattattct cccggttcac cgttgctcga tacccgtaaa ctctatagcc
    39061 aaagttggga cgccgggctg cgctataacg gcgaactgat taaatcacaa ctcattacca
    39121 gctatagcca tagcaaagat tacaactacg atccccatta tggtcgttat gattcgtcgg
    39181 cgacgctcga tgagatgaag caatacaccg tccagtgggc aaacaatgtc atcgttggtc
    39241 acggtagtat tggtgcgggt gtcgactggc agaaacagac tacgacgccg ggtacaggtt
    39301 atgttgagga tggatatgat caacgtaata ccggcatcta tctgaccggg ctgcaacaag
    39361 tcggcgattt tacctttgaa ggcgcagcac gcagtgacga taactcacag tttggtcgtc
    39421 atggaacctg gcaaaccagc gccggttggg aattcatcga aggttatcgc ttcattgctt
    39481 cctacgggac atcttataag gcaccaaatc tggggcaact gtatggcttc tacggaaatc
    39541 cgaatctgga cccggagaaa agcaaacagt gggaaggcgc gtttgaaggc ttaaccgctg
    39601 gggtgaactg gcgtatttcc ggatatcgta acgatgtcag tgacttgatc gattatgatg
    39661 atcacaccct gaaatattac aacgaaggga aagcgcggat taagggcgtc gaggcgaccg
    39721 ccaattttga taccggacca ctgacgcata ctgtgagtta tgattatgtc gatgcgcgca
    39781 atgcgattac cgacacgccg ttgttacgcc gtgctaaaca gcaggtgaaa taccagctcg
    39841 actggcagtt gtatgacttc gactggggta ttacttatca gtatttaggc actcgctatg
    39901 ataaggatta ctcatcttat ccttatcaaa ccgttaaaat gggcggtgtg agcttgtggg
    39961 atcttgcggt tgcgtatccg gtcacctctc acctgacagt tcgtggtaaa atagccaacc
    40021 tgttcgacaa agattatgag acagtctatg gctaccaaac tgcaggacgg gaatacacct
    40081 tgtctggcag ctacaccttc tgaaccacgt cccaccgtgc tggtgtttga ctccggcgtc
    40141 ggtgggttgt cggtctatga cgagatccgg catctcttac cggatctcca ttacatttat
    40201 gctttcgata acgtcgcttt cccgtatggc gaaaaaagcg aagcgtttat tgttgagcga
    40261 gtggtggcaa ttgtcaccgc ggtgcaagaa cgttatcccc ttgcgctggc tgtggtcgct
    40321 tgcaacactg ccagtaccgt ttcacttcct gcattacgcg aaaagttcga cttcccggtt
    40381 gttggtgtcg tgccggcgat taaacctgct gcacgtctga cggcaaatgg cattgtcgga
    40441 ttactggcaa cccgcggaac agttaaacgt tcttatactc atgagctgat cgcgcgtttc
    40501 gctaatgaat gccagataga aatgctgggc tcggcagaga tggttgagtt ggctgaagcg
    40561 aagctacatg gcgaagatgt ttctctggat gcactaaaac gtatcctacg cccgtggtta
    40621 agaatgaaag agccgccaga taccgttgta ttgggttgca cccatttccc tctactacaa
    40681 gaagaactgt tacaagtgct gccagaggga acccggctgg tggattctgg cgcagcgatt
    40741 gctcgccgaa cggcctggtt gttagaacat gaagccccgg atgcaaaatc tgccgatgcg
    40801 aatattgcct tttgtatggc aatgacgcca gaagctgaac aattattgcc cgttttacag
    40861 cgttacggct tcgaaacgct cgaaaaactg gcagttttag gctgatttgg ttgaatg
//
LOCUS       DAJTZL010000012        40665 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.012, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000012 DAJTZL010000000
VERSION     DAJTZL010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40665
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.012"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            209..1306
                     /gene="mltA"
                     /locus_tag="OO544_000588"
     CDS             209..1306
                     /gene="mltA"
                     /locus_tag="OO544_000588"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HCR6766273.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            1383..2189
                     /gene="tcdA"
                     /locus_tag="OO544_000589"
     CDS             1383..2189
                     /gene="tcdA"
                     /locus_tag="OO544_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HCR6766274.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSVGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(2240..2683)
                     /gene="csdE"
                     /locus_tag="OO544_000590"
     CDS             complement(2240..2683)
                     /gene="csdE"
                     /locus_tag="OO544_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HCR6766275.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVTENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(2683..3888)
                     /gene="csdA"
                     /locus_tag="OO544_000591"
     CDS             complement(2683..3888)
                     /gene="csdA"
                     /locus_tag="OO544_000591"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HCR6766276.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEDALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            4080..4307
                     /locus_tag="OO544_000592"
     CDS             4080..4307
                     /locus_tag="OO544_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HCR6766277.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            4658..5575
                     /gene="gcvA"
                     /locus_tag="OO544_000593"
     CDS             4658..5575
                     /gene="gcvA"
                     /locus_tag="OO544_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HCR6766278.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            5594..5989
                     /locus_tag="OO544_000594"
     CDS             5594..5989
                     /locus_tag="OO544_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HCR6766279.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            5982..7082
                     /gene="rlmM"
                     /locus_tag="OO544_000595"
     CDS             5982..7082
                     /gene="rlmM"
                     /locus_tag="OO544_000595"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HCR6766280.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVDMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPAEAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(7126..7857)
                     /gene="fucR"
                     /locus_tag="OO544_000596"
     CDS             complement(7126..7857)
                     /gene="fucR"
                     /locus_tag="OO544_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HCR6766281.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(7915..8337)
                     /gene="fucU"
                     /locus_tag="OO544_000597"
     CDS             complement(7915..8337)
                     /gene="fucU"
                     /locus_tag="OO544_000597"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HCR6766282.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            complement(8339..9757)
                     /gene="fucK"
                     /locus_tag="OO544_000598"
     CDS             complement(8339..9757)
                     /gene="fucK"
                     /locus_tag="OO544_000598"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HCR6766283.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTECHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIEQLISAQRLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGADQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYADSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTAQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            complement(9866..11641)
                     /gene="fucI"
                     /locus_tag="OO544_000599"
                     /pseudo
     CDS             complement(9866..11641)
                     /gene="fucI"
                     /locus_tag="OO544_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
     gene            complement(11674..12990)
                     /gene="fucP"
                     /locus_tag="OO544_000600"
     CDS             complement(11674..12990)
                     /gene="fucP"
                     /locus_tag="OO544_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HCR6766284.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQT
                     FNSFGAIIAVVFGQRLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPTLCFAVIFIFARFRSQTATN"
     gene            13537..14184
                     /gene="fucA"
                     /locus_tag="OO544_000601"
     CDS             13537..14184
                     /gene="fucA"
                     /locus_tag="OO544_000601"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HCR6766285.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRPIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYVATLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            14212..15360
                     /gene="fucO"
                     /locus_tag="OO544_000602"
     CDS             14212..15360
                     /gene="fucO"
                     /locus_tag="OO544_000602"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HCR6766286.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMHYNADFTGEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(15465..16220)
                     /gene="xni"
                     /locus_tag="OO544_000603"
     CDS             complement(15465..16220)
                     /gene="xni"
                     /locus_tag="OO544_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HCR6766287.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHDEMPALRAAFEQRGVPCWSTSGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(16332..17699)
                     /gene="sdaB"
                     /locus_tag="OO544_000604"
     CDS             complement(16332..17699)
                     /gene="sdaB"
                     /locus_tag="OO544_000604"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HCR6766288.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     NSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            complement(17757..19046)
                     /gene="sdaC"
                     /locus_tag="OO544_000605"
     CDS             complement(17757..19046)
                     /gene="sdaC"
                     /locus_tag="OO544_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HCR6766289.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            complement(19602..20966)
                     /gene="ppnN"
                     /locus_tag="OO544_000606"
                     /gene_synonym="ygdH"
     CDS             complement(19602..20966)
                     /gene="ppnN"
                     /locus_tag="OO544_000606"
                     /gene_synonym="ygdH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase"
                     /protein_id="HCR6766290.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLFLTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
     gene            complement(21078..21926)
                     /gene="queF"
                     /locus_tag="OO544_000607"
     CDS             complement(21078..21926)
                     /gene="queF"
                     /locus_tag="OO544_000607"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HCR6766291.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATCGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            21994..22539
                     /gene="syd"
                     /locus_tag="OO544_000608"
     CDS             21994..22539
                     /gene="syd"
                     /locus_tag="OO544_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HCR6766292.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            23161..23490
                     /locus_tag="OO544_000609"
     CDS             23161..23490
                     /locus_tag="OO544_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HCR6766293.1"
                     /translation="MTTHDRVRLQLQTLEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            23490..24272
                     /gene="truC"
                     /locus_tag="OO544_000610"
     CDS             23490..24272
                     /gene="truC"
                     /locus_tag="OO544_000610"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HCR6766294.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            24290..24739
                     /locus_tag="OO544_000611"
     CDS             24290..24739
                     /locus_tag="OO544_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HCR6766295.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            25174..26526
                     /gene="gudP"
                     /locus_tag="OO544_000612"
     CDS             25174..26526
                     /gene="gudP"
                     /locus_tag="OO544_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HCR6766296.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSHIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            26528..27868
                     /locus_tag="OO544_000613"
     CDS             26528..27868
                     /locus_tag="OO544_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HCR6766297.1"
                     /translation="MTTQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKRFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPGGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            27889..29229
                     /gene="gudD"
                     /locus_tag="OO544_000614"
     CDS             27889..29229
                     /gene="gudD"
                     /locus_tag="OO544_000614"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HCR6766298.1"
                     /translation="MSSQFTTSVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPYGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(29461..32217)
                     /gene="barA"
                     /locus_tag="OO544_000615"
     CDS             complement(29461..32217)
                     /gene="barA"
                     /locus_tag="OO544_000615"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HCR6766299.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            32274..33575
                     /gene="rlmD"
                     /locus_tag="OO544_000616"
     CDS             32274..33575
                     /gene="rlmD"
                     /locus_tag="OO544_000616"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HCR6766300.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVTVTEDKKQYARAKVVRRLSDSPGRETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHEVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPQLEALLPKVMACLGSLQVMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            33623..35857
                     /locus_tag="OO544_000617"
     CDS             33623..35857
                     /locus_tag="OO544_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HCR6766301.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            35935..36183
                     /gene="mazE"
                     /locus_tag="OO544_000618"
     CDS             35935..36183
                     /gene="mazE"
                     /locus_tag="OO544_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HCR6766302.1"
                     /translation="MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLII
                     EPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKEVW"
     gene            36183..36518
                     /gene="mazF"
                     /locus_tag="OO544_000619"
     CDS             36183..36518
                     /gene="mazF"
                     /locus_tag="OO544_000619"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HCR6766303.1"
                     /translation="MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTG
                     MCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKSIAWRARGATKKGTVAPEELQLI
                     KAKINVLIG"
     gene            36589..37380
                     /gene="mazG"
                     /locus_tag="OO544_000620"
     CDS             36589..37380
                     /gene="mazG"
                     /locus_tag="OO544_000620"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HCR6766304.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            37608..39245
                     /gene="pyrG"
                     /locus_tag="OO544_000621"
     CDS             37608..39245
                     /gene="pyrG"
                     /locus_tag="OO544_000621"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HCR6766305.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            39333..40631
                     /gene="eno"
                     /locus_tag="OO544_000622"
     CDS             39333..40631
                     /gene="eno"
                     /locus_tag="OO544_000622"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HCR6766306.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAADKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
BASE COUNT        10072 a         9840 c        10816 g         9937 t
ORIGIN      
        1 tccgtggatg cgcactatac tccgctcgcg ttttcatgca accttttttt taccccgatc
       61 acggatctgg catgattttg aacaaaaaca aatcactaaa gcctattttt tgtgcaaaat
      121 ttgcgtcagg gagaggctgc tcatttcatt aaacgcggcg gtttgttatc ttcgttgcgc
      181 cttatttttt aacctgaaga agagaacaat gaaaggacgt tgggtaaagt accttcttat
      241 gggcacggtt gtggcaatgc ttgccgcctg ctcttccaaa ccaaccgatc gcggacagca
      301 atataaagac gggaaattta cccagccttt ctctctggtg aaccagccag atgccgttgg
      361 cgcgccgatt aacgccggtg attttgccga gcaaattaac catatccgta attcgtcacc
      421 gcgtctgtat ggcaaccaga gtaatgttta taacgcggtg caagagtggc tgcgcgcagg
      481 cggtgatacc cgcaatatgc gccagttcgg cattgatgcc tggcagatgg aaggtgccga
      541 caactatggt aacgtgcagt ttacaggcta ttacacgccg gtaattcagg cgcgccatac
      601 ccgccagggc gagttccagt atcctattta ccgtatgccg ccaaaacgtg gtcgtctgcc
      661 gtctcgtgcg gagatctacg cgggggcatt gagtgataaa tatattctcg cttacagtaa
      721 ctccctgatg gataacttca ttatggatgt gcagggtagt gggtatatcg actttggtga
      781 tggcagtccg cttaactttt tcagctatgc agggaaaaac ggtcatgcct atcgcagcat
      841 tggtaaggtg ctgatcgacc gtggcgaagt gaaaaaagaa gatatgtcga tgcaggcgat
      901 tcgtcactgg ggcgaaacac acagtgaagc cgaggttcgc gagctgctgg aacagaaccc
      961 gtctttcgtc ttctttaaac cgcaatcttt tgcaccggtg aaaggggcaa gtgcggtgcc
     1021 gctggttggt cgcgcgtcag ttgcctctga tcgttccatt attccgccag gtactacctt
     1081 gctggcagaa gtgccgttgc tggataataa cggcaaattt aatggtcagt acgaactgcg
     1141 tctgatggtg gcgctggatg tcggtggtgc aatcaaaggc caacacttcg atatctatca
     1201 agggatcggg ccggaagccg gacaccgcgc aggttggtac aaccactatg gacgtgtctg
     1261 ggtgctgaaa accgccccgg gcgcaggtaa cgtctttagc ggctgatgtg ttattctgta
     1321 taccgttttt acggatacga cagggtgagg gataacctca ccctttttaa ttctgaggtg
     1381 ctatgtctgt ggtaattagt gatgcatggc gtcagcgttt tggtggcaca gcgcgtctgt
     1441 atggtgaaaa agcgttgcaa ctgtttgctg acgcgcatat ttgtgtggtt ggtatcggtg
     1501 gtgtcggttc ctgggcggcg gaagcgctgg cgcgcacggg gattggcgca atcacgctta
     1561 tcgatatgga tgatgtgtgc gtcaccaata ccaatcggca aattcacgct ctgcgcgata
     1621 acgtcgggct ggcaaaagcg gaagtcatgg cggagcgtat tcgccagatt aacccggagt
     1681 gccgtgtaac ggtggtggat gatttcgtga cgccggataa cgtagcgcag tatatgagcg
     1741 tgggttattc gtacgtgatt gatgccattg atagtgtacg gcccaaagcg gcgctgattg
     1801 cttattgtcg gcgcaataaa atcccgctgg tcacgaccgg tggcgcgggt gggcagattg
     1861 acccgacgca gattcaggtt accgatctgg cgaaaacgat tcaggatccg ctggcggcga
     1921 agttgcgcga gcgcctgaaa agcgattttg gcgtagtgaa aaacagtaaa ggtaagctcg
     1981 gcgtcgattg cgtgttttct actgaagcgc tggtgtaccc gcagtcagac ggtactgtgt
     2041 gtgcgatgaa agccacggca gaagggccga agcggatgga ttgtgcatct ggatttggcg
     2101 cggcaacgat ggtgaccgcc acctttgggt ttgttgcggt ttctcatgcg ctgaagaaga
     2161 tgatggcgaa agcggcgcgt cagggttaaa caatcaggtc ggatgagacg cgaacgtctt
     2221 atccgacccg gttctatgct taaacctgct tcgtcgcagc gataatcgcc tcgcttaacg
     2281 catttaaccc ctggctgcgt gaggcgctaa gttgcgcacg caatcccagc tcatcaaaca
     2341 atgccagcgg cgactgtgcc tgcaattcgg cggcggtttt cccctcaacg gcagtcaaca
     2401 gcaccgccag caggccgcgc acaatgcgcc cttcgctatc gccaaagaag tgcattttac
     2461 cgttttcagt cacggtatat cccagccaga cgcggttttc gcatccggca atctctttag
     2521 cctgcgcttt taactcgtct ggcaaagccg gaagctgttt ccccagcatg atcaactggc
     2581 gatatttatc ttcccattgc gacaacggtg cgaaggtatt gcgtaacgtt tctgcggtta
     2641 cggttgtgcc gaacggatgt ccggcgaatt gcgggttcgt cattaatcca ccaataattc
     2701 cagcgcgcgg tcaacggcat tcaccagcgc atccacatca ctctttgtat tatatggcgc
     2761 aaaagaggcg cgcagtgtgc cggttacgcc taattctgcc agtagcggct gagcgcaatg
     2821 ttgcccggcc cgcagggcaa taccgtactc cgccagcagc gtcaccatat cgctgtgatg
     2881 aacgccagca aaatcaaagg ccagcaggct ggaatcctgg cagcggaatg agcggaagcc
     2941 aggacgtttt gccagcgcat cttccgccag cgtcgccagg ctgcgactcc aactttctgc
     3001 ctggttgata tcgtaatctg ccagccattc cagcgccgcg cttaatccta tgacaccagc
     3061 cacatttggc gttcctgctt ccagtttcca cggcgcagat tgagtcgtga agccgtcaaa
     3121 actcacttcg tgaatcattt tgccgccacc cagccagggc gacatcgctt ccagcagttc
     3181 tgatttgcca tacagcacgc cgatacccgt cgggccatac agtttgtgac cagagaaagc
     3241 atagaaatcg atatctagtt gctgaacatc tgcggggaaa tgcaccgccc cctgagcacc
     3301 atcaaccatc accaccatcc cggctgaatg agcaaaggta atcgctcgcg ccagatccgg
     3361 gcaaccgcca gtaacgttcg acatctgacc caacgccaga atccgactac ggggagtaat
     3421 cagttctggc aacaaatcga catccggcag tcgctgcgca ttaagcggca atttcaccac
     3481 tttggctcca gtttgttggg cgaccatcag ccaggggacg aggttagcgt ggtgttctgc
     3541 cacgctgaca ataatctcat cgcccggttg cagacgcgga cgcgcatagc attgtgccac
     3601 catgttgatg gattcagtgg tgccgcgcgt ccagacgata gttttatcat ccggtgcatt
     3661 cagtaattgc gccactttct ctcgtgcagc ttcataacgc gcggtcaggc gttgggcttc
     3721 ggcaaactgg ctgcgatgga cgtttccggc gctcaggctg taaaactgtc gagtggcttc
     3781 aaccacggct tcaggtttaa gcgcggtcgc ggcgctatcg agatagacgc ccgcatcctg
     3841 tagtgcggga aactgggcgc gaaactgcgc gggattaaaa acgttcatgg tactcctcgg
     3901 cttgatgcac cgatcgtgac gcaaaattca ctttgataca aggagagtgc taaccatcag
     3961 aattatctgg atatcctggc gaagccgcgt aagtaacata tgctgaatag tgttaggcgt
     4021 taattttagc ctgttaatca atactattaa gatttattaa tcgctttaag gagaagatga
     4081 tgaaaaagac tgccgcaatt atttctgcct gtatgctgac ttttgccctg agcgcctgtt
     4141 ccggttcgaa ctatgtgatg cacaccaatg acggacgtac catcgtctct gacggcaaac
     4201 cacagactga taacgatacc ggtatgattt cgtataaaga cgctaatggc aacaaacagc
     4261 agatcaaccg tactgacgtg aaagagatgg tcgaactgga tcagtaattc gcgatcgcaa
     4321 ggtaaaaaaa agcaccgcaa ttaggcggtg ctacattaat cactatggac agacagggta
     4381 aatgtacagg aagtgaaaaa aggtagcttt gctaccatgg tctgaatcgc agaccaattg
     4441 caaacacaac aacacaacat cacaaccgta agccaaaagt tcaccagaac acgcattccg
     4501 ataaaacttt tcgttccggc tcaggaagtg ccgccactat aggtatttgc tggtagaagc
     4561 tcaacggaca atttataatg gctcagatta aaaaaactaa taggttacac agtgtgatct
     4621 aattgttaaa ttaatttaac atcaaagttt aaaagccatg tctaaacgat taccaccgct
     4681 aaatgcctta cgagtttttg atgccgcagc acgccattta agtttcactc gcgcagcaga
     4741 agagcttttc gtgacccagg ccgcagtaag tcatcaaatc aagtctcttg aggatttttt
     4801 ggggctaaaa ctgttccgcc gccgtaatcg ttcactcctg ctgaccgagg aagggcaaag
     4861 ctatttcctc gatatcaaag agatattttc gcaattaacc gaagcgacgc gtaaactcca
     4921 ggcccgtagc gccaaggggg cgttgacggt cagtttactc cccagtttcg ccattcattg
     4981 gttggttccg cgactttcca gctttaattc agcttatccg ggaattgacg ttcgaatcca
     5041 ggcggttgat cgtcaggaag ataagctggc ggatgatgtt gatgtggcga tattttatgg
     5101 tcggggcaac tggccggggc tacgggtgga aaaactgtac gccgaatatt tattgccggt
     5161 gtgttcgccg ctactgctga caggcgaaaa acccttgaag accccggaag atctggctaa
     5221 acatacgtta ttacatgatg cgtcacgccg tgactggcag acatataccc gacagttggg
     5281 gttaaatcat atcaacgttc agcaagggcc aatttttagt catagcgcca tggtgctgca
     5341 agcggctatt cacgggcagg gagtggcgct ggcaaataac gtgatggcgc aatctgaaat
     5401 cgaggccgga cgtcttgttt gcccgtttaa tgatgttctg gtcagtaaaa acgcttttta
     5461 tctggtttgt catgacagcc aggcagaact gggtaaaata gccgcctttc gccaatggat
     5521 cctggcgaaa gccgctgctg aacaagaaaa attccgcttt cgttatgaac aataatttac
     5581 gtagggtacg accatgacca gccgttttat gctgattttc gccgccatta gcggcttcat
     5641 ttttgtggct ctgggcgctt ttggcgcgca tgtgttaagt aaaaccatgg gggccgttga
     5701 gatgggctgg atccagaccg gcctcgaata ccaggcgttt catacgctgg cgatcttagg
     5761 tctggcggtg gcaatgcagc gtcgcatcag tatctggttt tactggagta gcgttttcct
     5821 cgcgttaggc acggtgttgt tcagcggcag cctttattgc ctggcgctgt cccatctgcg
     5881 tttgtgggca tttgtcacac cggttggcgg cgtgagcttc ctcgcgggct gggcgttaat
     5941 gttagttggt gctatccgtt taaagcgcaa gggcgtaagt catgaataag gttgtattgc
     6001 tgtgccgtcc gggctttgaa aaagagtgcg ccgcagaaat taccgataaa gccggccagc
     6061 gggaaatttt cggttttgcc cgcgtgaaag agaatgcggg ttatgtcatt tatgaatgtt
     6121 atcaacctga tgatggcgat aagttaatcc gtgagctgcc gttcagttca ttaatttttg
     6181 cccgccagtg gtttgtggtg ggggaactct tgcagcattt gccgccagaa gatcgtatta
     6241 cccccattgt cgacatgttg cagggcgtag tagagaaggg cggtgaactg cgtgttgaag
     6301 ttgccgatac caacgaaagc aaagagttgc tgaaattctg ccgtaaattc accgtgccgc
     6361 tacgcgctgc cttgcgcgat gccggggtgc tggcgaacta tgaaacgccg aagcgtccgg
     6421 ttgtgcatgt attctttatt gcaccaggct gctgctatac cggttactca tacagcaaca
     6481 ataattcgcc gttctatatg ggcattccgc gcctgaaatt tccggcagag gcgccgagtc
     6541 gttccacgct caaactggaa gaggcatttc atgtgtttat tcctgcggat gagtgggatg
     6601 aacgcctggc gaacgggatg tgggcggtgg atttaggcgc ttgccctggc ggctggacct
     6661 accaactggt gaagcgcaac atgtgggttt attccgtcga caacggcccg atggcgcaaa
     6721 gtctgatgga taccgggcag gtgacgtggc tgcgggaaga cggtttcaaa ttccgtccga
     6781 cacgcagcaa tatctcctgg atggtatgcg atatggttga aaaaccggct aaagttgcgg
     6841 cattgatggc gcagtggctg gttaatggct ggtgccgtga aaccattttc aatctcaaac
     6901 tgccgatgaa aaaacgctac gaagaagtat cacacaatct ggcgtatatt caggcacagc
     6961 ttgatgaaca tggcataaat gctcagattc aggcgcggca gttgtatcac gatcgcgaag
     7021 aagtgacggt gcacgtccgc cgcatctggg ctgcggtggg tggtcgtcgc gacgagcgat
     7081 aacaggcaag gccggggcgt cgtcttcggc ctgctaccct ttccgtcagg ctgttaccaa
     7141 agaagttgca acctggcgct catcagaaat aatgtgcgtt acctcatcca gatgcccgat
     7201 gcgggcttcc cctgaacgat taaatttact tttatcaatc aataacaacg attgcgcggc
     7261 acgttttaat agcatcgatt tgtaatcagc gttgatcgca ttggagtccc acagtgcgcc
     7321 gctgctatcg atcccttcac aagaaaaaat aaacagatcg atttccagcg atttaagttg
     7381 ggaaatcagc gagggattga cgtaacagcc atatttgcgc tcaagtgtgc cgccggaact
     7441 gatcagttga atgcgttcgc gtttaccgag ttcatggcaa atcggatggc tattggtgaa
     7501 gacctgaatg ttgatgtcag gcaactggcg tgccagatac cagcaagttg aactggcgtc
     7561 taaggctatc accatccctt cttcaatcca cgcgagcgcc tcgcgcgcga tatctgcttt
     7621 atgcgcataa tggcttttca gcctgatgtg aaagggatcg ccactgtctt gattttgacg
     7681 gtggatatat ttagcgcgtc catgattgcg cagaatttta ccctgcgtct gtaattcatt
     7741 gagatcgcga cgaatggttt ctttactgac ctttagctgt tcagagagag cttccgtggt
     7801 caggctggta tggttcagca gcaggtcgac tatcgcttgc tggcgtgccg ctttcatatt
     7861 tttcacccca tcattcgata accgcccgcg tcatgcgggc gtaccgatat gagattacgg
     7921 tgttacccct ttttttaaaa gaatattccc gtacttcgct cgttcgcctg tgataacgat
     7981 cgcaaaggct ttttgcgccc gttcataaaa cgcaaaacga ttgatgcgga tgatgtcagg
     8041 acacggggct tgtagtgaaa gcgcattacg gtaacgtcgt tctacttcag gatcgagagt
     8101 gtcaccttct accgctgcca tcatcaccag cggcggggca taactgtcca gctcaaataa
     8161 tgggataatc gcttggagca agtcgctcac caacaggcca tcagcgcgga tcacctgcgg
     8221 tcccatcgaa tgggcgggaa agtgagcatc ggaaaaaata atttcatctc catgtcccat
     8281 ctctgccagc actttcaata gttcgggaga aattaacggc gaaattgttt tcagcatttc
     8341 acacttcctc tataaattca ggttcagttt gcgggtagaa ataacggtac tgataatgaa
     8401 tctgtgcacg ggcttcttcc gggctgttaa attcccctac gccataccaa ccgaacagcg
     8461 cagctcctgc gacggtcgtt tcggcatcgt cgagaacttt taccggaata tcaagcatat
     8521 tggctttaat ctgattccac aatgtgttgc gacttcctcc accgactaac aataattcag
     8581 aggctttaaa gtgcccgatt ttttccagca tctgtagatt gcgctgtaat tgcgcagtta
     8641 acccttccag cgccgcgcga tagaaatgcc cccgcgtggt attaagcgtc actccttgcc
     8701 agccagcgtt ctgacacgac aataaatcac actgcatttt cacaccatcc gcgccaggtg
     8761 cgatcagacg agcttcttca atcaacattt gccagggtgt ttcagccgtc cagaacagtt
     8821 ttctcaccca ttccagcacg ccggatgcca gccattgcat acctgggtta tacaaccctg
     8881 cctggctatc cagttcgcag gtagaatcgg cgtactgact taacagcgaa gtatcaacct
     8941 gggcgctacg gaccattaaa atttcccatg taccggaaga gagcacgggt tcattttggt
     9001 cggcaccagc gccaaaaagg gcgaactggg tatcgtgacc tgcggaaatc accggtatgc
     9061 caacgggtaa gccgagcatt gctgcggcgc tgttctgtag cgtaccaatc tgttcacccg
     9121 cttccaccag acgagggaag agtcggcgtg gaataccggt ggcttgtaaa atttgcggac
     9181 tgaaatcgcg ttgctggata tccagcatct ggctggttcc ggccatcgtg atatcagtag
     9241 tgaattcgcc ggttaaacgg tggttaatca gcgacgaaat aaagagccag gcgtgcgcgc
     9301 gttccagcag ttgtggatga ttttctttca accacaccaa cttatataac gtattgaaac
     9361 taaaggctcc gacgccagaa atagcctgca accgctgtgc ggagatcaac tgttcaatat
     9421 tgtccataac cgctgctgtt cgcggacatt tccagctaat gatcggatag agcagattgc
     9481 cttgcttatc taccagagcg ccatccacac caaaggtggt gacggcgata ccgcggatgt
     9541 ggcattcagt cagttcacta ttgatttgcc gacagcaatc agcaaagcgt tgcaaaatgg
     9601 cgtctaaaga ccactggtgc caggtgttgt tttccatcgc gatatcgctg gcattaggcg
     9661 ttgaggcgcg ggcaacaatt tttccctgcc gattaaccgc gatggccctg acattggtcg
     9721 cgccacagtc gagtaccagg ataacttctt gtttcataat cgctcctgca atatagccgg
     9781 ataacattgc ttatccggct aaccactctt gcttgttcag gcccggtagc cggagcgacc
     9841 gggcatcaca tcagggagta atgtattaac gcttgtacaa cggaccgtag ttctggcaag
     9901 cgcggtaatc ctggccttca atatccatgc cgtgcgcagc ccaggcagaa ggacgataca
     9961 ctttggtctc ttcaacgttg tgcatacata ccgggatacg cagcatggag gcgagagtga
    10021 taaagtctgc gccaacgtgg ccgatggtca gaaccccatg gttagcgccc cagttcgcca
    10081 ttaccgagta cacatccgta aacggccctt taccggtgag gcgcggtgca aaccaggtgg
    10141 ttggccaggt tgagttggtg cgtttgttga ggatgtcatg cacatccttc ggcaattcca
    10201 cgctccagcc ttccgcgatt tgcagtaccg atcccaggcc tttgatgatg ttgacacgag
    10261 tcatggtgaa cgggacgccg ccttcggtaa ggaagcggga agagtaaccg ccgccacgga
    10321 agtattcgtg gatcgccggg caccattcgg tagcggcgag gcaagcgtca gcctcttgct
    10381 gagagatttc ccagtgtggc ttcatcgtcg ggttaccttc gctgtcgcgt tgtttacagg
    10441 aaccgtccag cgcagcagaa cctgagttga tcaaatggat gatgccgtgt tctgccagtc
    10501 catcgagttt atgccccgtt acacgctcaa ttgcttctgg tgaccagtag gtacgcacat
    10561 cggcaaatac ctgagcggtg ccggtgagct ggtgacccat tagcattgcc acgccgttaa
    10621 gactgtcgtt ttcggtcgcc acgacaaagg gttcgcgcac gccattccag tcaaatgaac
    10681 tgttgaggat cgcttcggcg gtgtcaccat tgggatattg atcggtccag tgacgttgcc
    10741 cctggaagcc cgcagcgatg gcgttgtagc caagtgattc ttccacgcga ccaatatcgg
    10801 ccagtttgct gttgccttgc atcatgtcgc ggatacacat cgccatcagt aaactttcgc
    10861 gcagaactgc gcggctttgc tcggcattgc gttgatactg tttgttattt tcatcttcgc
    10921 catagcggaa gtttttatca gcccaggtca gtgccatttc cagttcggct tcgtcgtaaa
    10981 tcttctgatc gatacggcga cgcagttcgg tcatatccac cgcctggact ttcattccca
    11041 gccaggattc aaagaagttg tgatcaacaa tagaaccggc gatacccatc gaaacgccgc
    11101 ccagcgacag atagctttta cctttcatgc tggcgacggc caaaccggcg cgggcaaagc
    11161 gcagcagttt ttcttcaaca tcggcaggaa tcgatgtgtc atcggcatcc tgaacgtcat
    11221 gaccgtaaat ggagaatgcc gggatgcctt tctggctgtg agctgccaga gccgctgcca
    11281 ggtaaacagc gccgggacgt tcagtgccgt taaagcccca aatggctttc gggcgggttg
    11341 gatccatgtc gatggtttca ctgccatagc accagcaagg cgttaccgta atggtgaggc
    11401 ctacattcta gctgctgaat ttttcttcgc aagcagcggc ttcagccata cccgcgatac
    11461 aggtatcgga aatgacacac tcgacggcag ctccgcaggc atggcgcagt ttctcggtca
    11521 gcagtgcggc cgtagctttc gccatattca ttgtttgttc ttcaagcgac tcacgaacac
    11581 ccatgcgacg accgtcaata accgggcgga taccaatttt cggtaagctg atttttttca
    11641 ttacagattc ctcactttat tcggaaaata tgttcagtta gttgccgttt gagagcggaa
    11701 acgggcaaag ataaagatga ccgcgaagca gagtgtgggg atcagttcag cagtggggat
    11761 gttgcccgcc gcgtcactga caaaacccat gaccggagtg acaataccgc cgccaataat
    11821 ggtcataacg atgaaggacg aaccgtattt ggtgtcctgg ccgagattct taatgcccag
    11881 cgagaagatt gttgggtact gaatcgacat aaaggcgctg cataaagtca gggctattaa
    11941 gcccacatga ccgccagcga aggctgagat caggcacagt gccatagcga ttaatgcgta
    12001 ggcggccagg actttgtgtg gtgcgaagcg actgatgagc caggtaccgg tgaaacgacc
    12061 aataaagaag cacaccatgg ttccggttaa atagttagcg gcaaagcctg cagtcatacc
    12121 tggaatttct tctacagcgt agcgaatcaa atagctccag caggccgttt gtgcgccaac
    12181 gtagcagaat tgcgctaata ctgcccagcg ccagtggcga atacgcgcta ggcgagaaag
    12241 cgatgcggag aacgatcctt gtttggcgtc actgtgatta tcactctgca atgccgggaa
    12301 tttcgtcagc atgatcagca gggcgaccag taacacgata gccacgatga tcatataagg
    12361 tgtctgtacc gataatacca ggctgtgttt atacgcactc aattgctctg gagacatttt
    12421 atcgagaacg tcttgcgatt gatgtggcac gttagacaaa ataagtcttt gcccaaagac
    12481 aaccgcgata attgcgccaa acgagttaaa tgtttgcgca agatttaagc ggaagtgacc
    12541 actgctttcc ggccctaata ccgtaacaaa agggtttgcg gcagtttcca gacaacctaa
    12601 tccggctgca ataataaata ggccaactaa aaataaggtg tagttcatta tttctgcagc
    12661 gggccagaat aatgcagcgc ccagggcata taaaaataac ccagtaataa ttcctgcttt
    12721 ataactgagt tttttcatca atatcccagc aggaattggg ataatgaaat aaccaaagta
    12781 aaaggccgat tggatcaggc cagcctggaa atttgtcagc gtaaaagcct gctggaattg
    12841 aggtaataaa atgtcgttaa ggttattggc taccgcccaa agaaaaaaca gtgagcacag
    12901 cagcgcgaat ggaataatgt aacttctgct ttgccctgca tctttatcta ccgcacggta
    12961 actctgcgtt tgtattgatg tgtttcccat agcatcctct taggttcaga agcttaagca
    13021 cgtaatcggt tatgttttca aaagtgcaaa accagcgagt tcgggcttca tatcagtgaa
    13081 tatttgcctg tctgaaatga actcatggat tcattttcaa ataaaaaacg ctctgtaata
    13141 tgacggcggt cacacttatt agctggaagg tgactttatg tgactaccat cactttaata
    13201 atcgaaattt catgcccgtt tcagaaaata aatcataaaa aacacaaacc agatgactgt
    13261 attaattgtt tatgccgaaa acgggcattc ctggcttggg tgaatagaaa aatgaccgtt
    13321 ttgataaata tttttaagag cggtcatttt taatggggtt ataaaaagag aacttgcagt
    13381 tatttactgt gattagttga gctaggtcac aaaatataag cttgtgaatt tctttcttgt
    13441 taatcaggaa gaagatggtg ggtaattaaa cggctaattc aatagtgtga aaggaacaac
    13501 attattgccc tgttttgaat cagagagagg tagctaatgg aacgaaataa acttgctcgt
    13561 cagattattg acacttgcct ggaaatgacc cgcctgggac tgaaccaggg gacagcgggg
    13621 aacgtcagtg tacgttatca ggatgggatg ctgattacgc ctacaggcat tccatatgaa
    13681 aaactgacgg agtcgcatat tgtctttatt gatggcaacg gtaaacatga ggaaggaaag
    13741 ctcccctcaa gcgaatggcg tttccatatg gcggcctatc aaagcagacc ggatgccaac
    13801 gcggttgttc acaatcatgc cgttcattgc acagcagttt ccattcttaa ccgaccgatc
    13861 cccgctattc actacatgat tgcggcggca ggtggtaatt ctattccttg cgcgccttat
    13921 gcgacctttg gaacacgcga actttctgaa catgttgcgc tggctctcaa aaatcgtaag
    13981 gcaactttgt tacaacatca tgggcttatc gcttgtgagg tgaatctgga aaaagcgtta
    14041 tggctggcgc atgaagttga agtgctggcg caactttacg tggcgaccct ggcgattacg
    14101 gacccggtgc cagtgctgag cgatgaagag attgccgtag tgctggagaa attcaaaacc
    14161 tatgggttac ggattgaaga gtaatttcgt aaagcaacaa ggagaaggat gatggctaac
    14221 agaatgattc tgaacgaaac ggcatggttt ggtcggggtg ctgttggggc tttaaccgat
    14281 gaggtgaaac gccgtggtta tcagaaggcg ctgatcgtca ccgataaaac gctggtgcaa
    14341 tgcggcgtgg tggcgaaagt gaccgataag atggatgctg cagggctggc atgggcgatt
    14401 tacgacggcg tagtgcccaa cccaacaatt actgtcgtca aagaagggct cggtgtattc
    14461 cagaatagcg gcgcggatta cctgatcgct attggtggtg gttctccaca ggatacttgt
    14521 aaagcgattg gcattatcag caacaatccg gagtttgccg atgtgcgtag cctggaaggg
    14581 ctttccccga ccaataaacc cagtgtaccg attctggcaa ttcctaccac agcaggtact
    14641 gcggcagaag tgaccattaa ctacgtgatc actgacgaag agaaacggcg caagtttgtt
    14701 tgcgttgatc cgcatgatat cccgcaggtg gcgtttattg acgctgacat gatggatggt
    14761 atgcctccag cgctgaaagc tgcgacgggt gtcgatgcgc tcactcatgc tattgagggg
    14821 tatattaccc gtggcgcgtg ggcgctaacc gatgcactgc acattaaagc gattgaaatc
    14881 attgctgggg cgctgcgagg atcggttgct ggtgataagg atgccggaga agaaatggcg
    14941 ctcgggcagt atgttgcggg tatgggcttc tcgaatgttg ggttagggtt ggtgcatggt
    15001 atggcgcatc cactgggcgc gttttacaac actccacacg gtgttgcgaa cgccatcctg
    15061 ttaccgcatg tcatgcacta taacgctgac tttaccggtg agaagtaccg cgatatcgcg
    15121 cgcgttatgg gcgtgaaagt ggaaggtatg agcctggaag aggcgcgtaa tgccgctgtt
    15181 gaagcggtgt ttgctctcaa ccgtgatgtc ggtattccgc cacatttgcg tgatgttggt
    15241 gtacgcaagg aagacattcc ggcactggcg caggcggcac tggatgatgt ttgtaccggt
    15301 ggcaacccgc gtgaagcaac gcttgaggat attgtagagc tttaccatac cgcctggtaa
    15361 atgcgctgat gtgataatgc cggatacgac gctagccgcg tcttatccgg tatataagtg
    15421 cattaaattc aatgtgttga taatcgtttg catccggctc gccgttaccg taccaaccgc
    15481 aattgctgta aattgccgtc gatatgcaaa tcggtttgta agcgggcaat atcgcggcac
    15541 agaaacgcca tctctttatg ggtttctaat tttttgcgcc acttttcggc aaccgcatcc
    15601 agattctcat atatcccttc cagactctga aactcgacca gcagctgcgt ggcgcttttt
    15661 ggtccgattc ccgcaacacc cggtaccttt gaactgctga tccccgcaag tccccagtaa
    15721 tcgggcaact gctgcggttg aacgccaaat tctttatcga taaatggcgc atccagccaa
    15781 cgtttctgga agtaatcacg aatacgtaat gtcggtgaaa gtaactgaca gtagccttta
    15841 tctgtcgaaa caatcgttgc ctgatgcccg gcctgtgtca ctttgaccgc cagcgtggcg
    15901 gctaagtcat cggcttcgtt gccgctggtt gaccagcacg ggacgccgcg ttgctcaaag
    15961 gcggcgcgta atgcaggcat ctcgtcgtgc aactcttccg gcattggcgg tcgacccgct
    16021 ttgtaatctg gtaaacgctg atgacgccag ccgctactgc ggttttcatc atcaaaaacg
    16081 gcgaccgcgt gggttggctg gctgtgcata atgagctgat cgagcgcatg ctggcaggtt
    16141 tcgacacagg gcgatccctg aacggcatga atgcgacgaa taagattcag tgcatcgaca
    16201 ataagcaaat gaacagccac gataaccccc ttaaagatca ggcgataagg gtaacattcg
    16261 atgccggaac aggctacggc tggatgagaa atcgggaaga ggcctcgcaa aacgaggcct
    16321 ctggagagcg attaatcgca ggcaacgatc ttcattgcca ggccgccgcg agaggtttcg
    16381 cggtacttgg cgttcatatc tttacctgtt tcgtacatgg tttcgataac tttatcgagg
    16441 cagacgcgcg gctcgctggt acggcgtagc gccatacgtg cggcgttcac cgcttttact
    16501 gccgcaatgg cgttacgctc gatgcatggc acctggacct gtccggcgac cgggtcacac
    16561 gtcagaccga ggttgtgctc catggcgatt tccgccgcga tgcacacctg cgccgggctt
    16621 gcacctaaca gttctgccag accagccgcc gccattgagc acgccacacc aacttcaccc
    16681 tggcagccga cttcagcacc agaaattgac gcgttcatct tataaagaga accaatggca
    16741 ctggctacca gcaggtaacg agccagtgag ttagcgttca cttcgcggat gaacttgtcg
    16801 tagtacgcca gcactgccgg gataatcccg cacgcaccgt tagtcggcgc agtcaccacg
    16861 cgtccgccag cagcgttctc ttcgttcact gccagtgcaa acatgttgat ccagtcaaca
    16921 accgccatcg ggtcagtggt ggttttatcc tggctgacca gcatccggcg tagtgccgca
    16981 gcacggcgtg gaacgcgcag tttgccaggc aacacgcctt cggtggaaat accgcgctca
    17041 ataccgccac gcatgacttc ccatacgttc gccaggtgct gttccagctc ttctttgctg
    17101 tgcagcgcca gctcattttt catcatcagg ccagagagtg acagcccggt ttcctgacaa
    17161 tgtttttgca gatcggctgc tgaactgtac ggataaggaa cttcaaccgg tgcgctattc
    17221 tgctggccaa aatgctcttc atcaacgata aagccaccgc caatagagta gtaagtctgg
    17281 ctgtaaacga ctttatcgcc cgccagcgcg gtaatgcgca taccgttttc atgcagagaa
    17341 aggttgtctg catggaagtt catgcactga tcaaccggga actccacttc atgctgaccg
    17401 tttgccagca tcaggcgacc atgagtattc acatcctgaa taaaaccagg gatggaatcg
    17461 atatccacgg tatccggcag gttacccgcc aggcccataa taatggcgat atcagtgtgg
    17521 tggcctttac cggtcagaga gagcgagccg tacacgtcaa ccaccacgcg ggtcacgtct
    17581 ttaagcagat tacgggcaat cagatcgtcg gtaaattgtt tacccgcttt cattggtcca
    17641 acggtatgag aactggaagg gccaatgccg attttgaaaa tatcgaatac gctaatcata
    17701 ggaaatacat cgtgttaaaa cggaggaagc gccgcccgaa agcggcgcga aaggacttag
    17761 ctgaacagag agtagaagat tgcggagatt gcaatcagac ccatcacgac aacgaatacg
    17821 ttgctgatgt gaccgctgta cttacgcatt gccggtactt tctgaattgc gtacatcggc
    17881 atcaggaaca ggatcatcgc gatgattggg ccgcccaggg tttcaatcat acccaggatg
    17941 ctcgggttca gggtggcaac aatccaggtc gttaccagca tgaacagcgc agtgatacgg
    18001 ttcagcttgt tgatttcgat agacttacct ttaccacgca gagatttaat caccataccg
    18061 ttgaagcctt cacgtgcgcc caggtagtgg ccgaggaagg atttggtgat agcgataatc
    18121 gcgataatcg gagccatcca cgcgataacc ggtgcgttaa agtggttagc caggtaagac
    18181 agaatcgaga tgttctgctc tttagccgca gccaggtctg ccggagtcag gctcagtaca
    18241 cagctgaaga cgaagaacat tacggtcagc accatcatga tgtgtgcgaa tgccaggatc
    18301 ttcgagcatt tctgttctgc catatcgccg tactcttcac gcttcgcaac ggcgaaagaa
    18361 gagatgatcg gagagtggtt gaaagagaac accataaccg gaattgccag ccacagggtc
    18421 atccacagac cgtttccggt tgcagatgca gtgtccagag acagcgtttc cagtgctgcg
    18481 ccgttccact gcgggatcag gtacagagcc agcagcatca gtacgccaac aaacgggaat
    18541 accagaatac tcattgcttt aacgatcatc tgctcaccga agcgaacgat ggtcatcata
    18601 cccacgatca ggatcagcga cagaatcgca cgcggcggtg gtgtcatacc cagctggtga
    18661 gacatgaagc tttcaactgt attggtgatt gccacgctat aaaccagcag gatcgggtag
    18721 atagcgaaga agtagagcag ggtaatcagt ttacctgcgc caataccaaa gtgttcttct
    18781 acaacctcgg tgatgtcttc gcctgggttt ttaccagaca gtacgaagcg agtcaggccg
    18841 cggtgagcaa aaaatgtcat cgggaacgca aggatagcca tgatgatcag cgggatcata
    18901 ccgccaacac cggcgttgat tggcaggaac agcacgcccg cgccgattgc cgtgccgtaa
    18961 aggcccagca tccacatggt gtctgtcttg cgccaggcac tacgagagtc tttcgacgca
    19021 atcgtgctgg tttgagtcgt ttccatctat ttctcctgga ggaatgcaaa taattcagct
    19081 tttagccagt tcagctcggg aaggtgcctg accgtaaaaa tgaaatctgt ctatcggcga
    19141 tggcttaatc atttaccgcc gtaataattt gagggcggaa agatacacac aagttatgta
    19201 tctatcagtg atcttgatcc caaatacaat aatctacttg gaagatagat tatcccaacg
    19261 ctttactctg taaatcaaaa gtaaaagaga atttaagggc aatatttgcc cattaaacga
    19321 catctgactt cttccggggc gcgcaatata tgtaggttga actgaaaaaa tcaatacttt
    19381 tagttaatca tggcgataat ttctttttca tagcgataaa atgtggttta tgaaaatagg
    19441 gaaacgtttg cgttacaaca taaattgtat gacatattaa ctgcaatgat atttatcgcg
    19501 tgagggtggt aagaaacatt catataacaa atgaaataaa ttgatgggta tgtgaccaaa
    19561 aacgagaaaa cccggcaaca aacgtcaccg ggggagggag attacgtgca gatttcgtag
    19621 caagggatgt aggctgagcc tggcaacttc atacgatgct gggcaacaaa accctgtagc
    19681 aggtcgtcca tacgacgcat aatctcttta tcgccgttga ttttgtaagg accaaactct
    19741 tcaatggcgc gaataccgac ttcttttacg ttacccgcca caataccgga gaacgcacgg
    19801 cgcagatcgg cagccagcac ttcaacaggt tgatccgggt aaagcttcag attagccata
    19861 ttctcgtgag acggctcaaa cggcatttgc aaatctggcg caatgcgcat tgaccagtta
    19921 aagctgtagg catcgcctgt gtcgcggcga ttttctttca ccagcggcat cgattttttc
    19981 atctgacgag cgacttcagc ggcgtcatca atgatgatgc ggtaatggcg gcgcgcgttt
    20041 tcacccaacg tatgcacgac aaactcgtcc agtacgcgga agtagtcggc gctctctttc
    20101 gggccggtga ggaacaatgg taaaacctga tctttgttgg ccgggttcat taaaattccc
    20161 agcaaataca gcaactcttc tgccgtaccc acaccgccag ggaagataat gatgccgtga
    20221 gcgatacgga caaacgcttc cagacgtttt tcgatatctg gcatgatgat caattcgttg
    20281 accagcgggt taggcggttc agcggcgata atcgacggct ctgtcatacc aataaaacga
    20341 ctgtctttgt aacgctgctg tgcgtgtccg accgcagcac ctttcatcgg cgcttccatc
    20401 gctcccggac cacagccggt gcagatattc agctcacgca ggcccagctg attcccgacg
    20461 cgacgggcat acaaatactc gttttcgtta attgagtgtc cgccccagca gaccaccata
    20521 tttggcgctt caccgacatg cagtgcgcga gcgttacgca agatggaaaa gaccaggtta
    20581 gtgatgtgga cggagttgtc gagattcagg ttgggaaaac gaacggtgtt atggatttgc
    20641 ccgtaaacga agagaatgtc acgcagaacc gcgaacaagt tggcctgcaa cgcgcgaata
    20701 attcgcccat cgacaaaagc ctcttccggg ggattaatca gttccagctt tacgccgcgt
    20761 tcacggcgca ggacgttaat atcgaaattt tcaaaacgag acagcaattc tttgctgtta
    20821 tcggtcaaac taccggagtt cagtacggca agtgaacagt tgcgaaacag ttgatagagg
    20881 tcgctgctgg cggtgcgttt aagcatatcc acttccagct gcgacaacat atccatggag
    20941 ccaagcgggc taatatgtgt aatcaagaaa aactccttat gggacgaata tcgtcattcc
    21001 ctttgattac aatagccctg cctgatgtcc tttcacaacc ttctgcgtga atccagcacg
    21061 caaaattgag aaaaaattta ttgccgaacc agtcttgtgg tcgatgggac aaaatcgcta
    21121 ttactgcgcc acgggttaat gtccagaccg ccacgacggg tataacgtgc gtaaacgctc
    21181 aatttttctg gctggcagaa gcgtaacagg tcattaaaga tgcgttccac gcactgttcg
    21241 tggaactcgt tgtgatgacg gaatgagacc aggtaacgca gcagtttttc tctgtcaatt
    21301 tggcgtccac gatactgaat ttggatcgaa ccccaatctg gttgatgggt gatcaggcag
    21361 tttgatttca gcaggtggct gacaagcgtc tcttccacta ctttttcacc acaggtggca
    21421 ttctccagat agtcagtagt gaattcatag ttatcgatag tgatatcctg gtcatcaatg
    21481 caagtgccat taaaatgacc tatcggctgg ccttccagtt catcaagacg atataacgcc
    21541 acgctaatct taccctgagc gcaagtgctt aagtcgcgct ccagcgtctg gcgcacctca
    21601 tcccagttat taaaacgcgt ctggttaaaa ctgttgagat agagcttaaa actcttcgac
    21661 tcaatcagat ttacgctggt gtaatcaagt tcaacatgac cgacagcgac ctgcggcaaa
    21721 cctttcgcat tcagccagga aagttcatac agcgtccaga tatccgtacc gtgaaaaggc
    21781 aggttatccg ctttcagacc cagcgggtcg cgattcaggc tgcgtggaac gccttgcagt
    21841 aggctggcgt cataggtatc ccggtaatcg gttgattttc caagagtcag gcccgcaagt
    21901 gcctgatggt ttgcataaga agacatgttt caccgtcata aaccagataa actaaacgac
    21961 aagtgtatct ggtttaagtg aagagagaaa ttagtggacg atttgaccgc acaagccctg
    22021 aaagatttta ctgcacgcta ctgcgatgcg tggcatgaag agcataaaag ctggccgtta
    22081 agtgaggaac tgtacggtgt tccttcaccg tgcattattt caactaccga agatgccgta
    22141 tactggcaac cccagccgtt taccggggaa caaaatgtaa acgcggttga acgcgctttt
    22201 gatattgtga tacaacccac aattcatacc ttttatacga ctcagtttgc cggggatatg
    22261 cacgcgcagt ttggcgatat caaactgaca ttgctgcaaa cctggagtga agacgacttc
    22321 cgtcgggtgc aggaaaatct gattggtcat ctggtgactc agaaaaggct aaaactgccg
    22381 cccacgctat ttatcgcgac gctggaagaa gagttagagg tcatctcggt gtgtaatctc
    22441 tccggtgaag tgtgcaaaga gacgctggga acacgtaaac ggacgcattt agcctcaaat
    22501 cttgcggaat tccttaatca acttaagcct cttctgtaat ccaaataccc catctggttg
    22561 tgagagatct cttacagacc ctgtaggaga tcgccaggaa ataagcgaat acttaaaaag
    22621 ataagaatcg ttattttcat ttaaaatcaa aatgttgatt gttaattctt aactttcata
    22681 tgaaattttc cttaaggcat tgtctgtaag cgccttgtaa gacttcgcga aaaagacgat
    22741 tctatcttcg tcgacaggga gtcagacaac gaagtgaaca tcaggatgat gacacttctg
    22801 caggacacac caggatggtg tttcagggaa aggcttctgg atgaagcgaa gaggatgacg
    22861 caggacgcgt taaaggacac ctccaggatg gagaatgaga accggtcagg atgattcggt
    22921 gggtcaggaa ggccagggac acttcaggat gaagtatcac atcggggtgg tgtgagcagg
    22981 aagcaatagt tcaggatgaa cgattggccg caaggccaga ggaaaagttg tcaaggatga
    23041 gcagggagca acaaaagtag ctggaatgct gcgaaacgaa ccgggagcac tgtgaataca
    23101 gtgctccctt tttttattcc tgctatcctt cgcggcagtt tttcttattg aagttgcttt
    23161 atgaccactc atgaccgcgt tcgtctccag ttgcagacgc ttgaagcgtt actgcgtgaa
    23221 catcagcact ggcgaaacga tgaaccccag ccgcatcaat ttaatagtac ccaaccgttc
    23281 tttatggaca ccatggaacc actggagtgg ttgcagtggg tgctgatccc gcgaatgcac
    23341 gatttgttaa ataacaacca gccgttaccg ggcgcttttg ctgttgcacc ctattatgaa
    23401 atggcgctgg cgacggacca tccgcaacgc gcgcttattc tggctgagtt ggaaaaactg
    23461 gatgctcttt ttgcggatga tgcgagctaa tgctggaaat actctatcag gatgaatggc
    23521 tggttgcggt aaataaaccc tccggctggc tggttcaccg tagctggctg gatcgcgacg
    23581 agaaagtagt ggtcatgcaa accgtgcgtg accagatagg acagcatgtt tttactgctc
    23641 atcgtctgga ccgacccact tctggtgtgt tgttgatggg attatccagc gaggccggac
    23701 ggctgctggc acaacagttt gaacagcacc aaatccagaa acgttaccat gcgattgtgc
    23761 gcggctggtt gatggaagaa gcggtgctgg attatccact ggtggaagaa ctggacaaaa
    23821 tcgctgataa atttgcccgc gaagataaag gcccgcagcc agcagtgacg cattatcgcg
    23881 gtctggcgac cgtagaaatg ccggtagcga ccggacgtta cccgaccacg cgctacggcc
    23941 tggtggaact ggagccgaaa accggacgca aacatcagct tcgccgacat cttgcccatt
    24001 tgcgtcatcc gattattggc gatagcaaac atggcgattt acgccagaat cgcagcggtg
    24061 ctgagcattt tggcctccag cggttaatgc tacatgccag tcagttgtca ttgacgcatc
    24121 cttttactgg cgagccgctg actattcacg cgggcctgga cgacacctgg atgcaggcgt
    24181 tatcacaatt tggctggcgc ggtctgcttc ctgaaaatga aagggttgag ttcagtgcgc
    24241 catcgggtca ggatggagag ataagttcct aatccaggga gaaaacggta tggcggaaat
    24301 tggtattttt gtcggcacca tgtacggaaa ttcgctgtta gtggcggaag aagcagaagc
    24361 aattctgacc gcacagggcc acaaagcaac ggtatttgaa gatcctgaat taagcgactg
    24421 gctgccttat caggataagt atgttctggt ggttacgtcc acgaccgggc agggcgacct
    24481 tcctgatagc attgtgccgc tctttcaggg aatcaaagat agtctgggct tccagccgaa
    24541 tctgcgttat ggcgtgattg cgctcggcga cagcagttat gtaaatttct gcaatggcgg
    24601 caaacagttc gatgccttgt tgcaggaaca gagcgctcag cgggttggcg aaatgctgtt
    24661 gattgacgcc agtgagaacc cggaaccgga aacggaatca aatccgtggg tcgaacagtg
    24721 gggcacgctg ttgtcctgat atgttcagcg agcggtaaat gtcgttttag cggtgctgaa
    24781 tcgaatcttt tcaggcaaat gccagtaaaa actgcttcat agcgcggatt tttactggcg
    24841 tttgcctgga gtcaagcgat ccatttcata ctcttcttta tttcttcgtt ttaacccttc
    24901 ctttcttgtt cttgttttca tttccgtgaa gtggattcca ccgtccaggg ctaatgccaa
    24961 aatcgggcct cattgaacgc attaatgttg tgttgttgca cggtgagccg ctatggcgcg
    25021 ctttttatac tgctattgcc agatataaac acgcgccgta ttcggcgaac gacctataaa
    25081 aacggcaaaa aacaccctac gtcacctctg atttcctggc tatgtcgcag tccagagtga
    25141 gcgtggctaa cgcgaatttt caggagtgca acaatgagtt ctttaagtca ggctgcgagc
    25201 agtgtggaaa aacgcacgaa tgctcgttac tggatagtgg tgatgttgtt tatcgtcaca
    25261 tccttcaact acggcgaccg cgctacgctt tctattgccg gttcggaaat ggccaaagat
    25321 atcggccttg atcccgtggg aatgggctat gtgttctctg ccttctcatg ggcttatgtt
    25381 atcgggcaga tccctggtgg ttggttgctg gatcgttttg gttcaaaacg cgtctacttc
    25441 tggtcgatct ttatctggtc gatgtttacc ttgctgcaag gcttcgtcga tatctttagt
    25501 ggattcggca ttatcgttgc cctgtttact ctgcgcttcc tggtcgggct tgctgaagcg
    25561 ccatctttcc ccggcaacag tcacattgtt gcggcctggt ttccggcgca ggaaagggga
    25621 acggcggtgt cgatttttaa ctccgctcaa tacttcgcaa cggtgatctt cgcgccgatt
    25681 atgggctggc tgacgcatga agtgggctgg tcacacgtct tcttctttat gggcggtctg
    25741 gggattgtca tcagctttat ctggttgaaa gtcatccacg agccaaatca acatccgggg
    25801 gtaaatcaga aagagctgga gtacatcgcc gcgggtggcg cgctgatcaa tatggatcag
    25861 caaaacacca aagttaaagt gccgttcagc gtgaagtggg ggcagatcaa acaactgctc
    25921 gggtcacgga tgatgatcgg cgtttatatc ggtcagtact gtatcaacgc cctgacttac
    25981 tttttcatta cctggtttcc ggtttatctg gtgcaggcgc gtgggatgtc gattctgaaa
    26041 gcgggctttg tggcttccgt tccggcggtt tgcggtttta tcggcggtgt gctgggtggg
    26101 attatttccg actggctgat gcgtcgcacg ggatcgctga acattgcgcg taaaacaccg
    26161 atcgtaatgg gcatgttgct gtcgatggtg atggtgttct gcaactacgt caacgttgag
    26221 tggatgatca tcggctttat ggcgctggcc ttcttcggta agggcatcgg ggcgctgggt
    26281 tgggcagtaa tggcagatac cgcgccaaaa gagatcagcg gtctttccgg tggcctgttc
    26341 aacatgttcg gtaacatttc tggcatcgtc acgccaatcg caattggtta tatcgttggc
    26401 acgactggct cctttaatgg ggcgctgatt tatgttggtg ttcatgcctt aatcgcggta
    26461 ctgagctacc tggtgctggt gggcgatatc aagcgtatcg agctgaaacc tgtcgcgggg
    26521 caataagatg acgacacaat ccagtcctgt tattactgat atgaaagtca ttccggtcgc
    26581 cgggcacgat agcatgttgc ttaatattgg tggggcacat aacgcatatt tcacccgcaa
    26641 tattgtggta ctcaccgata acgccgggca taccggcatt ggtgaagcgc cgggcggaga
    26701 ggtgatttat caaacactgg tcgatgctat tccgatggtt ctgggccagg aagttgcgcg
    26761 cctgaataaa gtggttcagc aggtgcataa aggtaatcag gcagccgatt ttgatacctt
    26821 cggcaaaggt gcctggactt ttgaattgcg cgttaatgcc gtggcggcgc tggaagccgc
    26881 cttgcttgac ctgctaggta aggcgctgaa tgttccggtc tgcgaactgt tggggccagg
    26941 caagcaacgc gagactatta ccgtcctcgg ttatctgttt tatatcggtg atcggaccaa
    27001 aaccgatctt ccttatctgg aaaatacgcc gggcaaccat gagtggtatc agttgcgcca
    27061 tcagaaagcg atgaacagcg aagccgttgt gcgtctggcg gaagcctcac aggatcgcta
    27121 tggctttaaa gatttcaaac ttaagggcgg ggtgttacct ggcgagcaag aaatcgacac
    27181 tgttcgtgca ttgaagaaac gcttccctga tgcgcggatt accgttgatc ccaacggtgc
    27241 ctggctactt gatgaagcca tttctctatg caaagggctg aatgatgttc ttacctatgc
    27301 cgaagatcca tgcggcgcag aacagggttt ctccggtcgt gaagttatgg cggagtttcg
    27361 gcgggcgacc ggcttgcccg tcgcgactaa catgatcgcc accaactggc gcgaaatggg
    27421 tcatgcggtg atgctcaatg cggtagatat tccacttgcc gatccgcact tctggacgct
    27481 ttccggtgca gtccgtgtgg cgcagctttg cgacgactgg gggctgacct ggggctgcca
    27541 ttctaataac catttcgata tctctctggc gatgtttacc catgtgggcg cggcggcacc
    27601 gggtaatcct accgctatcg atacccactg gatttggcag gagggcgatt gtcgcctgac
    27661 caaaaatccg ctggagatta aaaacggaaa aattgccgtt cctgatgcgc ccggtctggg
    27721 cgtggaactg gactgggaac aggtacaaaa ggcacatgag gcctataaac gtctgcctgg
    27781 cggtgcgcgt aacgacgcag gtccgatgca gtacctgatc cccggctgga cctttgaccg
    27841 taaacgtccc gttttcggcc gtcattgatt ctgaaaaagg acataaatat gagttctcaa
    27901 tttacgacgt ctgttgttac tgaaatgcag gtcatcccgg tggcgggtca tgacagtatg
    27961 ctgatgaatc tgagtggggc acacgcaccg ttctttacgc gtaatattgt gattatcaaa
    28021 gataattccg gtcacactgg cgtaggggaa attcccggcg gcgagaaaat ccgtaaaacg
    28081 ctggaagatg cgattccgct ggtggtaggt aaaacgctgg gtgaatacaa aaacgttctg
    28141 acgctggtgc gtaatacttt tgccgatcgt gatgctggtg ggcgcggttt gcagacattt
    28201 gatttgcgta ccactattca tgtagttacc gggatagaag cggcaatgct ggatctgctg
    28261 gggcagcatc tgggggtaaa cgtggcatcg ctgctgggcg atggtcaaca gcgtagcgaa
    28321 gtcgaaatgc tcggttatct gttcttcgtc ggtaatcgca aagccacacc tctgccgtat
    28381 caaagccagc cggatgactc atgcgactgg tatcgcctgc gtcatgaaga agcgatgacg
    28441 ccggatgcgg tggtgcgcct agcggaagcg gcgtatgaaa aatatggctt caacgatttc
    28501 aaactgaaag gcggtgtact ggccggggaa gaagaagccg agtctattgt ggcactggcg
    28561 caacgcttcc cgcaggcgcg tattacgctc gatcctaacg gtgcctggtc gctgaacgaa
    28621 gcaattaaaa ttggtaaata cctgaaaggt tcgctggctt atgcagaaga tccgtatggt
    28681 gcggagcaag gtttctccgg gcgtgaagtg atggcagagt tccgtcgcgc gactggcctg
    28741 ccgactgcaa ccaatatgat cgccaccgac tggcggcaga tgggccatac gctctcgtta
    28801 caatccgttg atatcccgct ggcggatccg catttctgga ctatgcaagg ttcggtacgt
    28861 gtggcgcaaa tgtgccatga atttggcctg acctggggtt cacactcaaa caaccacttc
    28921 gatatttccc tggcgatgtt tacccatgtt gccgccgctg caccgggtaa aatcaccgcg
    28981 attgatacgc actggatttg gcaggaaggc aatcagcgct tgaccaaaga accgtttgag
    29041 atcaaaggcg gtctggttca ggtgccggaa aaaccggggc tgggtgtaga aatcgatatg
    29101 gatcaagtga tgaaagccca tgagctgtat cagaaacacg ggcttggcgc gcgtgacgat
    29161 gcgatgggaa tgcagtatct gattcctggc tggacgttcg ataacaagcg cccgtgcatg
    29221 gtgcgttaag acagaaaacc aaaaccggct ctatctggag ccggcttgag actgatgcta
    29281 aacgcaaaat agcctgttgt gctatgtatc aggtctacat cattttttaa ttcattgaat
    29341 ttaattggtt ttgtaggccg gataaggcgt taacgccgca tccggcataa acacaggcac
    29401 tttgtcacca atctgaaacc agcgtcataa aaagccgatt gctactcgac aagacgtccg
    29461 ttacccgaga attttgctgg cttcgcgcgc gacattatcc atctcgtcca acagttccag
    29521 cagctccggc tccaaatctt cttctttagt accgctacgt agctgttgtt cgatcagttg
    29581 gcagagattc ttcatacgcg gcacaccgct atagccgcaa ctgccatgca gtttatgaat
    29641 caaatcaacc aggccttccg ggttttctcc aaccagctgc tcctcaactt tgttgcgaac
    29701 ttcaggcagg aaatcgagta acatttgcag catatcgcgc gctagatcgg tttttcctgc
    29761 tgcctggcgt agtgccagtt gccagtcgag ggtcgcattc gggttcacca caatttcgtt
    29821 gacttcgggc gtcacgacgc gagaggaaat accgctgcca ggcttgtagc gcaacaacaa
    29881 attatgcaat cgctcttctt caatcggttt cgccagataa tcgctcatcc ctgcgccaag
    29941 cagcttctct ttttgcccgg ccattgcatg cgccgttacc gcgataaccg gcgtttgttg
    30001 ctgatggggg agctggtgga tgagctcgca ggcccgaatg ccatccatgt caggcatttg
    30061 aatatccatt aagatcaaat cgaacggcat ctgtttcgcc cgttcaaccg cctgatgccc
    30121 gctatcgcaa agttccacat gttgcaccat atcttccagc aatgcgccga taagtttcag
    30181 gttagcgggg ttgtcatcaa ctgccatgac tgtcattgcc agcttgcttt catcggttac
    30241 aggcaaaagc gtgttttgtt tgtgatgaca aaattccgtc agggcaggca acaggcgcgt
    30301 aggtgttaat ggtttcagca gacacgcgcc gataccatct tgcttgagtt tttctgcatt
    30361 gacttgtgca tggcaaggaa gtgccagcat caggaaatcg gtcatcgata ccgctttcgc
    30421 taatcgctca tgttgcattg ttagcggctc gcggaaggtc accgcgatgc ctaacaacat
    30481 catgtcgtaa tgcgcgggag gcagcgcgga gaacgttggg ctataaacca cttccagcgg
    30541 cgtttcactt aaaatatcca gcgtgcattg cgctgctgcg gagtttggtt cgacataggc
    30601 caggcgctta ccggcgaggc actgggtgga tggcccttcg ataataatgt tcgggttcag
    30661 atcgagatta atgtggaacc agaaagttga accgcgattc ggctggctat ggaacgaaat
    30721 atcgccgccc atttcattaa ccagtttttg tgtaatcacc agccccagac cggtgccacc
    30781 atgacggcgg gaaatactgg catcagcctg tcggaaggcc tggaataagc gcgattgatc
    30841 gcgttcagga ataccaatgc cggtatcccg aatctgcact tcaatctgca ctttggtatt
    30901 actcagcgca cgtttttcta ccagaatatc aatgttgcca ttctcggtga atttaattgc
    30961 attccccacc aggttagtga tgatttgctg taatcgcagt gggtcgccga tcacgttatc
    31021 aggcacgtcg cttttaatat tgagcgtcag ttccaaccct ttatcgtgag aagaatgtgc
    31081 cagcagagta acgacttcat ccagcgtgct gcgtagtggg aatggaatac tttccagaat
    31141 cagcttacct gcttccagtt tcgagaagtc gagaacatca ttaataattg ccagcaaatt
    31201 atttgccgaa cgttcaatcg tattcaggtg atcgcgctgc gttggtgtta attctgtttt
    31261 cagcgtcagg cgggtaaagc caataacacc attcagtggt gtacgcagct cgtgtgacat
    31321 atttgccaga aactcggatt taatacgcgc cgcttcctgg gcgcgctttt tcgccagatc
    31381 taactcaacg ttctgaattt ccatctgctc cagcgtttca cgcagatcgg acgtcgcctg
    31441 gtcgatattg tgctgcatct cttcgtgata agcagccagc gacattgcca tcgagttgat
    31501 accgtttttc agcatatcca gctcgccgag cataaatcct tccacccggc tgtcgagttg
    31561 cccgcgacgg atgcggtcga cggtattcac catgttgcga atcggaccgg ttacatcgcg
    31621 cattaagcgc cagccaaaaa taagcgcaat accgatacaa aacagcatca tcacgctgga
    31681 aataaagatc tctttatatt gctgcaagcg aaccgattta agatccagct ccagcgcaat
    31741 atatcccaac atattttgac tatttttggc atcgctactg ggcgattcgt cgggggagta
    31801 actctcagaa ataatcggcg tgcggaggat cataatatcg ccgtcacgag tgacagtgag
    31861 ctggcgagga aacggcacgt tgctgccgag ctgcattgat gagggatcaa gatgaaaatt
    31921 ggaggtgaca aagagtcggt tattttcatc ataaaccgaa atcgcgcgaa caatatcgga
    31981 atggcgacga tgcagtacgc ttattaactg accgatagat tcgcgatttt gcaggctcat
    32041 gccatattca gtagaaactg caagcggctc aataatgctg gctccggcat cttccagttg
    32101 acgctgcaag tcgttatagc gatgcacaac gaaaaagata ctcagcaata aaccaataag
    32161 gacggtcggt gccaggatca gaatcatcat gcgtgcgcgc aggctgtagt tggtcatgga
    32221 gttccgttat gggacaatta aggtcacact gttaaattga gaaagtctcg ccaatggcgc
    32281 aattctactc tgcaaaacga cgcacgacga cgcgtcagat cataaccgtt tcagtcaacg
    32341 acctcgactc ttttggtcag ggcgtggcgc gacataacgg caaaacgcta tttatccccg
    32401 gattattgcc gcaggaaaac gcggaagtta ctgttactga agataaaaaa cagtatgccc
    32461 gcgctaaagt cgtacgccgg ttaagcgata gcccgggacg cgaaacgcca cgctgtcctc
    32521 attttggcgt atgcggcggc tgtcagcaac aacacgccag cgtggattta cagcagcgaa
    32581 gcaaaagtgc ggcactcgcc cgattaatga aacacgaagt ctctgaagtg atcgccgatg
    32641 ttccctgggg ctatcgccgt cgcgcgcgtt taagtctgaa ctacttaccg aaaacacagc
    32701 aacttcagat ggggtttcgc aaagcgggct ccagtgacat tgtcgacgtt aaacaatgcc
    32761 ccattttagt gccccaactt gaagcattgc tgcccaaagt catggcatgc ttgggcagct
    32821 tacaagttat gcgccatctt ggtcatgttg aactggtaca ggcaaccagc ggcacgctga
    32881 tgattttgcg ccataccgca ccgctaagtt cggcagatcg cgaaaaactg gaacgctttt
    32941 cgcattctga aggcctggat ctgtatctcg cccccgatag tgagatactc gaaaccgtct
    33001 ctggtgagat gccctggtat gactcaaatg ggttgcgctt aacttttagc ccgcgcgatt
    33061 ttattcaggt caatgcgggt gtgaaccaaa aaatggtagc gcgtgcgttg gaatggctgg
    33121 atgtgcaacc tgaagatcgc gtactggatt tgttctgcgg tatgggcaac tttacactgc
    33181 cattggcgac acaagctgcc agtgtggtgg gtgtagaagg cgttccggcg ctggtggaaa
    33241 aaggccagca gaatgcgcgt cttaatggct tacagaatgt gacgttttat cacgaaaatc
    33301 ttgaagaaga tgtcacaaag cagccgtggg cgaaaaacgg cttcgataaa gtgttgctgg
    33361 acccggcgcg agcaggtgcc gcaggtgtta tgcagcaaat tataaaactg gaacctattc
    33421 gtatagttta tgtatcctgt aaccctgcaa cgctggctcg ggatagcgaa gcgttattaa
    33481 aagcaggata taccattgcg cgactggcga tgctggatat gttcccacac acgggacatc
    33541 tggaatcgat ggtacttttc tcgcgcgtta aatagttgcg atttgccgat ttcggcaggt
    33601 ctggtcccta aaggagagga cgatggttgc ggtaagaagt gcacatatca ataaggctgg
    33661 tgaatttgat ccggaaaaat ggatcgcaag tctgggtatt accagccaga agtcgtgtga
    33721 gtgcttagcc gaaacttggg cgtattgtct gcaacagacg caggggcatc cggatgccag
    33781 tctgttattg tggcgtggtg ttgagatggt ggagatcctc tcgacattaa gtatggacat
    33841 tgacacgctg cgggcggcgc tgctgttccc tctggctgat gccaacgtag tcagcgaaga
    33901 tgtgctgcgt gagagcgtcg gtaagtcggt tgttaacctt attcacggcg tgcgtgatat
    33961 ggcggcgatc cgccagctga aagcgacgca cactgattct gtttcctccg aacaggtcga
    34021 taacgttcgc cggatgttat tggcgatggt cgatgatttt cgctgcgtag tcatcaaact
    34081 ggcggagcgt attgctcatc tgcgcgaagt aaaagatgcg ccggaagatg aacgtgtact
    34141 ggcggcaaaa gagtgtacca acatctacgc accgctggct aaccgtctcg gaatcggaca
    34201 actgaaatgg gaactggaag attactgctt ccgttacctc catccaaccg aatacaaacg
    34261 aattgccaaa ctgctgcatg aacggcgtct cgaccgcgaa cactacatcg aagagttcgt
    34321 tggtcatctg cgcgctgaga tgaaagctga aggcgttaaa gcggaagtgt atggtcgtcc
    34381 gaaacacatc tacagcatct ggcgtaaaat gcagaaaaag aacctcgcct ttgatgagct
    34441 gtttgatgtg cgtgcggtac gtattgtcgc cgagcgttta caggattgct atgccgcact
    34501 ggggatagtg cacactcact atcgccacct gccggatgag tttgacgatt atgtcgctaa
    34561 cccgaaacca aacggttatc agtctattca taccgtggtt ctggggccag gtggcaaaac
    34621 cgttgagatc caaatccgca ccaaacagat gcatgaagat gcagagttgg gtgttgctgc
    34681 gcactggaaa tataaagagg gcgcggcagc tggcggcgca cgttcgggac atgaagaccg
    34741 gattgcctgg ctgcgtaaac tgattgcgtg gcaggaagag atggctgatt ccggcgaaat
    34801 gctcgacgaa gtacgcagcc aggtctttga cgaccgggtg tatgtcttta cgccgaaagg
    34861 tgatgtcgtt gatttgcctg cgggatcaac gccgctggac ttcgcttacc acatccacag
    34921 tgatgtcgga caccgctgca tcggggcaaa aattggcggg cgcattgtgc cgttcaccta
    34981 ccagctgcag atgggcgacc agattgaaat tatcacccag aaacagccga accccagccg
    35041 tgactggtta aacccaaacc tcggttacgt cacaaccagc cgtgggcgtt cgaaaattca
    35101 cgcttggttc cgtaaacagg accgtgacaa aaacattctg gctgggcggc aaatccttga
    35161 cgacgagctg gaacatctgg ggatcagcct gaaagaagca gaaaaacatc tgctgccgcg
    35221 ttacaacttt aatgatgtcg acgagttgct ggcggcgatt ggtggcgggg atatccgtct
    35281 caatcagatg gtgaacttcc tgcaatcgca atttaataag ccgagtgccg aagagcagga
    35341 cgccgccgcg ctgaagcaac ttcagcaaaa aagctacacg ccgcaaaacc gcagtaaaga
    35401 taacggtcgc gtggtagtcg aaggtgttgg caacctgatg caccacatcg cgcgctgctg
    35461 ccagccgatt cctggagatg agattgtcgg cttcattacc caggggcgcg gtatttcagt
    35521 acaccgcgcc gattgcgaac aactggcgga actgcgctct catgcgccag aacgcattgt
    35581 tgacgcggta tggggtgaga gctactccgc cggatattcg ctggtggtcc gcgtggtggc
    35641 taatgatcgt agtgggttgt tacgtgatat cacgaccatt ctcgccaacg agaaggtgaa
    35701 cgtgcttggc gttgccagcc gtagcgacac aaaacagcaa ctggcaacca tcgacatgac
    35761 cattgagatt tacaacctgc aggtgctggg ccgcgtgctg ggtaaactca accaggtgcc
    35821 ggatgttatc gacgcgcgtc ggttgcacgg gagttaggca gaaatttgct cgtatctaca
    35881 atgtagattg atatatactg tatctacata tgatagcggt ttgaggaaag ggttatgatc
    35941 cacagtagcg taaagcgttg gggaaattca ccggcggtgc ggatcccggc tacgttaatg
    36001 caggcgctca atctgaatat tgatgatgaa gtgaagattg acctggtgga tggcaaatta
    36061 attattgagc cagtgcgtaa agagcccgta tttacgcttg ctgaactggt caacgacatc
    36121 acgccggaaa acctccacga gaatatcgac tggggagagc cgaaagataa ggaagtctgg
    36181 taatggtaag ccgatacgta cccgatatgg gcgatctgat ttgggttgat tttgacccga
    36241 caaaaggtag cgagcaagct ggacatcgtc cagctgttgt cctgagtcct ttcatgtaca
    36301 acaacaaaac aggtatgtgt ctgtgtgttc cttgtacaac gcaatcaaaa ggatatccgt
    36361 tcgaagttgt tttatccggt caggaacgtg atggcgtagc gttagctgat caggtaaaaa
    36421 gtatcgcctg gcgggcaaga ggagcaacga agaaaggaac agttgcccca gaggaattac
    36481 aactcattaa agccaaaatt aacgtactga ttgggtagtg ttactaactc acttctattc
    36541 tggtcacagg tttccacctg acagacccgc aattttcagg acaattcaat gaatcaaatc
    36601 gaccgtttgc tcactattat gcagcgcctg cgcgatccgg aaaacggctg cccgtgggat
    36661 aaagagcaga catttgccac cattgcgcct tacacccttg aagaaaccta cgaagtgctg
    36721 gacgccatcg cccgtgaaga ttttgacgat cttcgcggtg aactgggcga tctgctattc
    36781 caggtggtgt tttacgcgca aatggctcag gaagaagggc gctttgactt taatgatatt
    36841 tgcgctgcta ttagcgataa attagagcgt cgccatccgc atgtttttgc tgatagttct
    36901 gccgaaaaca gtagtgaagt gcttgcccgt tgggagcaaa tcaaaaccga agagcgcgcg
    36961 cagaaagcgc agcattcggc gctggacgat attcctcgta gtttaccggc tttaatgcgg
    37021 gcgcaaaaaa tccagaaacg ttgcgccaac gttggcttcg actggacgac gcttggcccg
    37081 gtggttgata aagtctacga agagatcgac gaggtgatgt acgaggcgcg gcaggctgtt
    37141 gtcgaccagg ctaaactgga ggaggaaatg ggggacctgc tgtttgccac ggttaatctg
    37201 gctcgccatt tagggacgaa agcagaaatc gcattgcaaa aagcgaacga aaaattcgag
    37261 cgtcgttttc gcgaagtgga gcgtattgtt gccgcgcgtg gactggaaat gacaggtgtt
    37321 gacctcgaaa caatggaaga agtctggcaa caggtaaaac ggcaggaaat tgatctctaa
    37381 gggaattacg cggtcaagcg ccatttgtgt cattttttaa atgacaagcg cttgatttgc
    37441 gtcaaaaaca tttaccccaa aggggctatt ttctcactcc tgatttcaat agtgcgctgg
    37501 cgaagaggag ggataatgaa agtttgtggc acaggtcatg ttcgggtata ctgctttccc
    37561 gtcctggtta ttccatcgtc ttttcaacct aacttctcag gttcagcatg acaacgaact
    37621 atatttttgt gaccggcggg gtcgtatcct ctctgggtaa aggcattgcc gcagcctccc
    37681 tcgcagccat tcttgaagcc cgtggcctca atgtgaccat catgaaactg gatccgtaca
    37741 tcaacgtcga tccaggtact atgagcccaa tccaacacgg ggaagtgttc gttactgaag
    37801 acggcgctga aaccgacctg gacctggggc actacgagcg tttcattcgt accaaaatga
    37861 gccgccgcaa caacttcacc acgggtcgta tctactctga cgttctgcgt aaagaacgcc
    37921 gcggtgacta cctcggcgca accgtgcagg ttattccgca catcactaac gcaatcaaag
    37981 agcgcgtgct ggaaggtggc gaaggtcatg acgtagtact ggtagaaatc ggcggtacag
    38041 taggtgatat cgaatccttg ccgttcctcg aagcgattcg ccagatggct gttgaaattg
    38101 gccgtgagca cactctgttt atgcacctga cgctggtgcc gtacatggca gcgtctggtg
    38161 aagtcaaaac caaaccgact cagcactctg taaaagagct gctctccatc ggtatccagc
    38221 ctgacatcct gatttgtcgt tcagatcgcg ctgttccggc gaacgaacgt gcgaagattg
    38281 cattgttctg taatgttccg gaaaaagcgg ttatttctct gaaagacgtc gattccatct
    38341 ataaaattcc gggcctgttg aaatctcagg ggctggacga ttatatttgt aaacgattca
    38401 gcttaaactg cccggaagca aatctgtccg aatgggaaca ggttatcttc gaagaagcga
    38461 atccggtaag tgaagtcacc atcggtatgg tcggcaagta cattgaactg ccggatgctt
    38521 ataaatcagt gatcgaagca ctgaaacacg gtgggctgaa gaatcgtgtc agcgtcaaca
    38581 tcaaactgat cgattcacaa gatgttgaaa cgcgcggcgt tgaaatcctt aaaggtctgg
    38641 acgcaatcct cgtacctggc ggtttcggct atcgtggcgt agaaggcatg attacgaccg
    38701 cgcgttttgc gcgtgagaac aatattcctt atctgggcat ttgcctgggt atgcaggtgg
    38761 cgttaattga ttacgctcgc catgttgcca acatggagaa cgccaactct acggaatttg
    38821 tgccagactg taagtacccg gttgtggcgc tgattaccga gtggcgcgat gaaaacggca
    38881 acgttgaagt tcgtagcgag aagagcgatc tcggcggtac catgcgtctc ggcgcacagc
    38941 agtgccagtt ggttgacgat agcctggttc gccagctgta caatgcgccg acaattgttg
    39001 agcgtcatcg tcaccgttac gaagtcaaca acatgctgtt gaaacagatt gaagatgcag
    39061 gtctgcgcgt tgcgggccgt tccggggatg atcagttggt cgagatcatc gaagttccga
    39121 atcacccgtg gttcgtggct tgccagttcc atccggagtt tacttctact ccacgtgatg
    39181 gtcacccgct gtttgcaggc tttgtgaaag ctgccagcga gttccagaaa cgtcaggcga
    39241 agtaagtaaa aaagttagaa cggcaacgcg taccttgggt acgcgttgtt tgtctggagt
    39301 ttcagtttaa ctagtgactt gaggaaaacc taatgtccaa aatcgtaaaa atcatcggtc
    39361 gtgaaatcat cgactcccgt ggtaacccga ctgttgaagc cgaagtacat ctggagggtg
    39421 gtttcgtcgg tatggcagct gctccgtcag gtgcttctac tggttcccgt gaagctctgg
    39481 aactgcgcga tggcgacaaa tcccgtttcc tgggtaaagg cgtaaccaaa gctgttgctg
    39541 cggtaaacgg cccgatcgct caggcactga ttggcaaaga cgctaaagat caggctggca
    39601 ttgacaagat catgatcgac ctggacggca ccgaaaacaa atccaaattc ggcgcgaacg
    39661 caatcctggc tgtatctctg gctaacgcca aagctgctgc tgccgataaa gggatgccgc
    39721 tgtacgagca catcgctgaa ctgaacggca ctccgggcaa atactccatg ccggttccga
    39781 tgatgaacat catcaacggt ggtgagcacg ctgacaacaa cgttgatatc caggaattca
    39841 tgattcagcc ggttggcgcg aaaactgtga aagaagccat ccgcatgggt tctgaagttt
    39901 tccatcacct ggcaaaagtt ctgaaagcga aaggcatgaa cactgcagtt ggtgacgaag
    39961 gtggctatgc gccgaacctg ggttccaacg ctgaagctct ggctgttatc gctgaagctg
    40021 ttaaagctgc tggttatgaa ctgggcaaag acatcactct ggcgatggac tgcgcagctt
    40081 ctgaattcta caaagacggt aaatacgttc tggctggcga aggcaacaaa gctttcacct
    40141 ctgaagaatt cactcacttc ctggaagaac tgaccaaaca gtatccgatc gtatctatcg
    40201 aagacggtct ggacgaatct gactgggacg gtttcgcata ccagaccaaa gttctgggcg
    40261 acaaaatcca gctggttggt gacgacctgt tcgtaaccaa caccaagatc ctgaaagaag
    40321 gtatcgaaaa aggtatcgct aactccatcc tgatcaaatt caaccagatc ggttctctga
    40381 ccgaaactct ggctgcaatc aagatggcga aagatgctgg ctacactgca gttatctctc
    40441 accgttctgg cgaaactgaa gacgctacca tcgctgacct ggctgttggt actgctgcag
    40501 gccagatcaa aactggttct atgagccgtt ctgaccgtgt tgctaaatac aaccagctga
    40561 ttcgtatcga agaagctctg ggcgaaaaag caccgtacaa cggtcgtaaa gagatcaaag
    40621 gccaagcata agactgactt tatctgattt aaaaatgcca gcctc
//
LOCUS       DAJTZL010000013        39658 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.013, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000013 DAJTZL010000000
VERSION     DAJTZL010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39658
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.013"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(40..115)
                     /locus_tag="OO544_000623"
     tRNA            complement(40..115)
                     /locus_tag="OO544_000623"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(80..82),aa:Lys,seq:ttt)
     gene            complement(119..194)
                     /locus_tag="OO544_000624"
     tRNA            complement(119..194)
                     /locus_tag="OO544_000624"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(159..161),aa:Val,seq:tac)
     gene            complement(344..419)
                     /locus_tag="OO544_000625"
     tRNA            complement(344..419)
                     /locus_tag="OO544_000625"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(384..386),aa:Lys,seq:ttt)
     gene            complement(584..1375)
                     /gene="cpoB"
                     /locus_tag="OO544_000626"
     CDS             complement(584..1375)
                     /gene="cpoB"
                     /locus_tag="OO544_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HCR6766307.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(1385..1906)
                     /gene="pal"
                     /locus_tag="OO544_000627"
     CDS             complement(1385..1906)
                     /gene="pal"
                     /locus_tag="OO544_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HCR6766308.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(1941..3233)
                     /gene="tolB"
                     /locus_tag="OO544_000628"
     CDS             complement(1941..3233)
                     /gene="tolB"
                     /locus_tag="OO544_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HCR6766309.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(3366..4598)
                     /gene="tolA"
                     /locus_tag="OO544_000629"
     CDS             complement(3366..4598)
                     /gene="tolA"
                     /locus_tag="OO544_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HCR6766310.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALLWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKIKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEEAA
                     KKAAADAKKKAEAEAAKAAAEAQKKAEAAAAALKKKAEAAEAAAAEARKKAAAEKAAA
                     DKKAAEKAAADKAAADKKAAAEKAAADKKAAAAKAAAEKAAADKKAAAAKAAAEKAAA
                     AKAAAEADDIFGELSSGKNAPKTGGGAKGNNASSAGSGNTKNNGASGADINNYAGQIK
                     SAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKIPKP
                     PSQAVYEVFKNAPLDFKP"
     gene            complement(4663..5091)
                     /gene="tolR"
                     /locus_tag="OO544_000630"
     CDS             complement(4663..5091)
                     /gene="tolR"
                     /locus_tag="OO544_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HCR6766311.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(5095..5787)
                     /gene="tolQ"
                     /locus_tag="OO544_000631"
     CDS             complement(5095..5787)
                     /gene="tolQ"
                     /locus_tag="OO544_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HCR6766312.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(5784..6188)
                     /gene="ybgC"
                     /locus_tag="OO544_000632"
     CDS             complement(5784..6188)
                     /gene="ybgC"
                     /locus_tag="OO544_000632"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HCR6766313.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYVPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPCALPKSIVAEFKQ"
     gene            complement(6338..6631)
                     /gene="ybgE"
                     /locus_tag="OO544_000633"
     CDS             complement(6338..6631)
                     /gene="ybgE"
                     /locus_tag="OO544_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HCR6766314.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(6631..6744)
                     /gene="cydX"
                     /locus_tag="OO544_000634"
     CDS             complement(6631..6744)
                     /gene="cydX"
                     /locus_tag="OO544_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HCR6766315.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(6759..7898)
                     /gene="cydB"
                     /locus_tag="OO544_000635"
     CDS             complement(6759..7898)
                     /gene="cydB"
                     /locus_tag="OO544_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCR6766316.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(7914..9482)
                     /gene="cydA"
                     /locus_tag="OO544_000636"
     CDS             complement(7914..9482)
                     /gene="cydA"
                     /locus_tag="OO544_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HCR6766317.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            complement(10330..12886)
                     /locus_tag="OO544_000637"
                     /pseudo
     CDS             complement(10330..12886)
                     /locus_tag="OO544_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415260.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
     gene            complement(12904..14820)
                     /gene="mngA"
                     /locus_tag="OO544_000638"
     CDS             complement(12904..14820)
                     /gene="mngA"
                     /locus_tag="OO544_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HCR6766318.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LEEVYRRESLGPTALGEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPPNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQALTLKRSDETRTVQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTTYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLMRWVKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            14989..15711
                     /gene="mngR"
                     /locus_tag="OO544_000639"
     CDS             14989..15711
                     /gene="mngR"
                     /locus_tag="OO544_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HCR6766319.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEILIF
                     EVIPADDFLQQQLQITAQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            complement(15815..16684)
                     /gene="sucD"
                     /locus_tag="OO544_000640"
     CDS             complement(15815..16684)
                     /gene="sucD"
                     /locus_tag="OO544_000640"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HCR6766320.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(16684..17850)
                     /gene="sucC"
                     /locus_tag="OO544_000641"
     CDS             complement(16684..17850)
                     /gene="sucC"
                     /locus_tag="OO544_000641"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HCR6766321.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            complement(17944..19161)
                     /gene="odhB"
                     /locus_tag="OO544_000642"
     CDS             complement(17944..19161)
                     /gene="odhB"
                     /locus_tag="OO544_000642"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HCR6766322.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            complement(19176..21977)
                     /gene="sucA"
                     /locus_tag="OO544_000643"
     CDS             complement(19176..21977)
                     /gene="sucA"
                     /locus_tag="OO544_000643"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HCR6766323.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKENMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRTASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            complement(22278..22994)
                     /gene="sdhB"
                     /locus_tag="OO544_000644"
     CDS             complement(22278..22994)
                     /gene="sdhB"
                     /locus_tag="OO544_000644"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HCR6766324.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYPLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            complement(23010..24776)
                     /gene="sdhA"
                     /locus_tag="OO544_000645"
     CDS             complement(23010..24776)
                     /gene="sdhA"
                     /locus_tag="OO544_000645"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HCR6766325.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLHRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            complement(24776..25123)
                     /gene="sdhD"
                     /locus_tag="OO544_000646"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             complement(24776..25123)
                     /gene="sdhD"
                     /locus_tag="OO544_000646"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HCR6766326.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            complement(25117..25521)
                     /gene="sdhC"
                     /locus_tag="OO544_000647"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             complement(25117..25521)
                     /gene="sdhC"
                     /locus_tag="OO544_000647"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HCR6766327.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            25902..26009
                     /locus_tag="OO544_000648"
     CDS             25902..26009
                     /locus_tag="OO544_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6766328.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            26215..27498
                     /gene="gltA"
                     /locus_tag="OO544_000649"
     CDS             26215..27498
                     /gene="gltA"
                     /locus_tag="OO544_000649"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HCR6766329.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            27888..28454
                     /locus_tag="OO544_000650"
     CDS             27888..28454
                     /locus_tag="OO544_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR6766330.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLRLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            28505..30963
                     /locus_tag="OO544_000651"
                     /pseudo
     CDS             28505..30963
                     /locus_tag="OO544_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
     gene            30978..31709
                     /locus_tag="OO544_000652"
     CDS             30978..31709
                     /locus_tag="OO544_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCR6766331.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            31706..32767
                     /locus_tag="OO544_000653"
     CDS             31706..32767
                     /locus_tag="OO544_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6766332.1"
                     /translation="MSAGKGVLLVICLLFLPLKSALALNCYFGSSGGSVEKSEAIQPF
                     AVPGNAKLGDKIWESDDIKIPVYCDNNTNGNFESEHVYAWVNPYPGVQDRYYQLGVTY
                     NGVDYDANQGKSRIDTNQCIDSKNIDIYTPEQIIAMGWQNKICSGDPANIHMSRTFLA
                     RMRLYVKIREMPPHDYQSTLSDYIVVQFDGAGSVNEDPTAQNLKYHITGLENIRVLDC
                     SVNFSISPETQVIDFGKFNLLDIRRHTMSKTFSIKTTKSQNDQCTDGFKVSSSFYTEE
                     TLVEEDKALLIGNGLKLRLLDENASPYTFNKYAEYADFTSDMLVYEKTYTAELSSIAG
                     TPIEAGPFDTVVLFKINYN"
     gene            32919..33965
                     /locus_tag="OO544_000654"
     CDS             32919..33965
                     /locus_tag="OO544_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCR6766333.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(33962..34753)
                     /gene="nei"
                     /locus_tag="OO544_000655"
     CDS             complement(33962..34753)
                     /gene="nei"
                     /locus_tag="OO544_000655"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HCR6766334.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFSNDLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLDIPRFSYATRGLVDE
                     NKHHGALFRFKVFHRDGEPCERCGSIIEKTTLSSRPFYWCPGCQH"
     gene            complement(34789..35523)
                     /gene="pxpA"
                     /locus_tag="OO544_000656"
     CDS             complement(34789..35523)
                     /gene="pxpA"
                     /locus_tag="OO544_000656"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HCR6766335.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQIMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALILVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQRGALIENEEQALAQTLEMVQHGRVKSITGEWATVTAQTVCLH
                     GDGEHALAFARRLRSTFAEKEIVVAA"
     gene            complement(35513..36445)
                     /gene="pxpC"
                     /locus_tag="OO544_000657"
     CDS             complement(35513..36445)
                     /gene="pxpC"
                     /locus_tag="OO544_000657"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HCR6766336.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGIGGLEGRLLKDGDRLPIGKSKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSSQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTDGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLVWRLHNEN"
     gene            complement(36439..37095)
                     /gene="pxpB"
                     /locus_tag="OO544_000658"
     CDS             complement(36439..37095)
                     /gene="pxpB"
                     /locus_tag="OO544_000658"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HCR6766337.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAHCGFSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            complement(37118..37861)
                     /gene="ybgI"
                     /locus_tag="OO544_000659"
     CDS             complement(37118..37861)
                     /gene="ybgI"
                     /locus_tag="OO544_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HCR6766338.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDV
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            38132..39613
                     /gene="dtpD"
                     /locus_tag="OO544_000660"
     CDS             38132..39613
                     /gene="dtpD"
                     /locus_tag="OO544_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HCR6766339.1"
                     /translation="MNKHASQPRTIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
BASE COUNT         9608 a        10666 c         9650 g         9734 t
ORIGIN      
        1 ttacctgaaa gatttctact cggggccaat ctttaagatt ggtgggtcgt gcaggattcg
       61 aacctgcgac caattgatta aaagtcaact gctctaccaa ctgagctaac gacccgagtg
      121 gtgggtgatg acgggatcga accgccgacc ccctccttgt aagggaggtg ctctcccagc
      181 tgagctaatc acccgatact gcgctggata ctacaaacta cttccaccga acccaccgta
      241 attagtggtg ggtcgtgcag gatgactcgc ttcgctcgcc ctacgggccg ttgctgacgc
      301 aacgttatcc tacacgctta acatctgtct tcgatgctaa aactggtggg tcgtgcagga
      361 ttcgaacctg cgaccaattg attaaaagtc aactgctcta ccaactgagc taacgaccca
      421 ctttttcgtt gctttcggtt tgtttgatat cccgtggcaa cggcggcata tattactgat
      481 ttcagacttg agcgcaacaa aaatttcgat gcagatcact caactgctta tgattcgcac
      541 gacacgacca gaaataatgc gacttctggt cgtgtgttat gcattacatc gcgttcagac
      601 gtttttgtgc ctgtttagcg ccatcagtac cagggtattt actgataacc tgctggtata
      661 cggctttcgc ttttgcggtg tcacctttgt cctgcatgat gacgccgact ttaaacatcg
      721 catcagcagc ctttggtgac ttcggatagt ttttcactac cgaagcaaaa tagtacgccg
      781 catcatcttt tttacccttg ttgtagttta actgacccag ccaataattg gcgtttggca
      841 ggtaagttga atcagggtaa tttttgatga aattctgaaa tgccaccatt gcgtcatcct
      901 ggcgggattt atcctgcacc agcgcaatag ctgcattgta atccgtgttt gcatcgccgc
      961 ttttcaccgg cgcgccagca ttcgcagtac cagcatcagc tgtcggcgtc gttgatgccg
     1021 ccgcaccgct ttgatcgctg ctggttgatt gcgccgctgc accaccgctg ctgaggctgt
     1081 cgatctgcaa caggatctgc ttttgccgct ccacgacctg attcagttga tactgatttt
     1141 cctgaatctg accacgcagg gaatcaatat cggattgatt atcagaaagt tgttgctgga
     1201 gttgggttaa aagctggctg tgagcattag aaatacgctc aagttgagtg acgcggtctt
     1261 cgaccgagcc tgagccgaca ctactgattg gtgcctgagc aaaagcggcc cagggggccg
     1321 ctataccaac cagtaacgac agactcaata gttgatgtct gaagttactg ctcatgcaat
     1381 tctcttagta aaccagtacc gcacgacggt ttttggagta tgccgcttcg tcatgaccca
     1441 gtactgcagg tttttcttta ccgtaagaaa cgatggagat ctggtctgca gaaacgcctt
     1501 taccctgcag gtacatctta acggcgttcg cacgacgttc acccagggag atgttgtatt
     1561 ccggagtacc acgttcgtcc gcgtgacctt ctacggtgac tttgtaagac gggttgctac
     1621 gcaggaagtt tgcatgtgca tccagcattt gagcgaagtc agaacggata tcgtacttgt
     1681 ccagatcgaa gtaaacgatg ttgttctgct gcagctgttg catttgcaga cgagcctgct
     1741 cttcggaaga catgttgccg ttgccgccgt tcgcatccat accagtgccg gcacccagca
     1801 tgccttcgct gccgtcattg ctggcgttct tgttggaaga acatgccgca attgccataa
     1861 caggcagagc aatcatcagc cctttcagca ctttgttcag ttgcatttca atgattcctt
     1921 tactattcaa ttaattatta tcacagatac ggcgaccagg cagggaattt gacctgtcca
     1981 tcagttgccg gaagacgcgc tttgaaacgc ccatctgtag aaaccaaatt cagcacggat
     2041 cccatcccct gagaagagct gtagattacc atagtgccgt taggtgccag acttggcgtt
     2101 tcatccagga acgtggacga cagaacttgt acgcctcccg ttgccagatc ttgtttggca
     2161 atgtgctgct gcccaccgtt ggagctgacc attaccataa atttaccgtc gctgctgaca
     2221 tccgcatcct ggttctgcga accttcccag gtaatacgtt gtggcgcacc gccgttgata
     2281 ttcactttat aaacctgtgg acgaccggcc tggtcagaag taaatgccag gttctggctg
     2341 tccgggaacc aggtcggttc ggtattgtta ctgcgaccat cagtcacctg gcggatctga
     2401 ccagaagcca aatccattac gtacaggttc aggctaccgg ttttcgacaa ggcgaatgcc
     2461 agtttgctgc cgtctggcga gaatgcaggc gcaccgttgt gacgcgggaa tgaagccacc
     2521 tgacgtacag cgccgtttgc cagcgtttgg ataactagcg cggaacgacc gctttcgaag
     2581 gtcacataag ccagtttaga accgtctggt gaccacgccg gagacatcag cggctgtggt
     2641 gaacggtgaa cgacaaactg gttgtaaccg tcatagtcag atacgcgcag ttcatacggg
     2701 aactgaccgc cgttggtctg aacaacgtag gcaatacggg tacggaacgc acctttaatg
     2761 ccggtcagtt tttcaaacac ttcatcactg gcggtatgac cagcatagcg cagccactgc
     2821 ttgttcactt tgtacgagtt ctgagcaagt acagtacccg gtgcgccgcc agtgtcaaca
     2881 agttgataag caacattgta agaaccatcc ggattcggag tgacctgacc gacaactaca
     2941 gcgtcaatac ccagcgcgga ccatgcagct ggttgtactt cctgcgcact acccggctgc
     3001 tgtggcagac gagcgcgatc taacggatta aatttaccgc tgttacgcaa gtcggcagca
     3061 acgatgccgc caatatcttc aggtgccgca ccagggcccg cccactggaa aggaacaaca
     3121 ccaataggac gaccggaatc tacaccgctg tcgatcacaa tgcggacttc agcatgcaga
     3181 actgatgccc acagtatgag aaaaccaaat gctactcgta atgcctgctt catcatatct
     3241 cccttatctg gaccgatggc ccacgataat ttagcagaat gttaacaaac taaaatacac
     3301 aaaactacca gaaccccgtg gcaaccggtg cctgatgttg accgtcggaa cagtcaacat
     3361 cgcgattacg gtttgaagtc caatggcgcg tttttgaaca cttcatatac tgcctggctt
     3421 ggtggtttcg ggatcttcgc aagtttagct gctgccaacg cagcctgaca aagtgcggga
     3481 tcgccacctt caggtttgat atccagtaac ataccatcgg gtgccagttt tatgcgcagc
     3541 gtacaggttt tgcctgcata ggacgatgcg tcatagaact tactttcgat agcagattta
     3601 atctgcccgg catagttatt gatatcggcc cctgatgcgc cattgttttt agtattacca
     3661 ctcccggcag acgaagcatt gttccctttc gcccctcccc ccgttttcgg tgcattctta
     3721 ccagagctta gctcaccgaa aatatcatct gcctctgcgg cagcttttgc tgcagcggct
     3781 ttttcagctg ctgcttttgc cgctgccgct tttttgtctg cggcggcttt ttctgccgct
     3841 gcttttgccg ctgccgcttt tttgtctgcg gcggcttttt ctgccgctgc tttcttatca
     3901 gctgctgcct tatcagcagc cgctttttct gctgcttttt tgtcggctgc agctttctct
     3961 gctgccgctt tctttcttgc ttcagctgca gcagcttctg ccgcttccgc tttcttcttc
     4021 agtgccgcag cggctgcctc ggcttttttc tgcgcttcgg ctgcggcttt ggcggcttct
     4081 gcttctgctt ttttcttcgc gtctgccgcc gctttcttcg ctgcttcttc cgcagcttta
     4141 gcatccgctt cggccttcgc tttagcatct gccgccgctt tcgctgccgc ctcttccgct
     4201 tgcttctgct ttaactcggc ctgttttgcg gcttcttcag cctgcttttt ctgttcctga
     4261 gcagctaacc gctctttctc aagttgcttc aggcgttcct gttcagccgc ttgtttctca
     4321 cgcagttctt cagcagcctg ctgttccttt atcttgcgct gctcatcaga acgcttcgcg
     4381 cttgattcct ggctttgcat gcgtttgtac tgctcaacta ccgcacctga atcaaccatg
     4441 acagcgtcga tggacgaacc accgccgcct ccggctgaag cttctatatt ctcatcgaac
     4501 gaactccaga gcagcgccgc aaataagatg acatgcagca ctgctgaaat aattatcgcc
     4561 cgcttgagct tgtcgttttg ttcggttgcc tttgacactc tcggtttcca aaaactgttc
     4621 gcctgttacc cgctctcttt caagcaaggg aaacgcagat gtttagatag gctgcgtcat
     4681 taaaccaacc gatttcacac ccgcactatg taacaagttc agtgctttaa ttatttcatc
     4741 gtaaggcaca tcttttgcgc caccgatcag aaagaccgtt ttcgggttgg ccttgaaacg
     4801 gctggacact tccgccacca cctgctctgg tggtaaacgc tccaggcgat ctttctcaac
     4861 caccacggtg tactgaccaa taccagacac ttcaacaatc actggcggat tatcgttact
     4921 gctcaccgcc tgtgattcag tagcgtctgg cagatcgacc tccacgctct gggtgatgat
     4981 gggcgctgtc gccataaaga tcagcaacag caccagcagt acgtccagca acggtacaat
     5041 gttgatttcg gacttgagat cgcgacgacc tcgtccacgc gctctggcca tggcttaccc
     5101 cttgttgctc tcgctaacgg taaacgcctg gcggtgcaga atcgcggtaa actcttccat
     5161 aaagttgtcg taattcagtt ccagtttgtt tacgcgctgg ttgaggcggt tataagccat
     5221 taccgccgga attgcggcga acagaccaat tgcagtcgca atcaacgctt ctgcgatacc
     5281 gggcgcaacc atttgcagtg ttgcttgttt taccgccccg agggcgataa aggcgtgcat
     5341 gatcccccag accgtaccaa acagaccaat atacgggctg atggagccaa ccgtaccgag
     5401 gaacggaatg tgcgtttcca gattttcaag ttcacggttc atggagatac gcatagcacg
     5461 cgacgcccct tccactacgg cttccggcgc atggctattg gcacgatgca ggcgcacaaa
     5521 ctctttgaac ccgctgtaaa atatttgttc cgaaccagtc agattatccc gtttcccctg
     5581 actctcttga tagaggcgag agagttcgat tccagaccag aatttatctt caaacgcttc
     5641 ggcttcgcgc gccgctgcgt taagaatacg ggtccgctgg ataataatgg cccaggatgc
     5701 gattgaaaaa ccaatcaaaa tcaacatgat aagtttaacc agaaggctag ccttcaggaa
     5761 caaatcaagg atattcatgt cagtcactgc ttaaactccg cgacaataga cttgggaagc
     5821 gcacaaggct tcattttgag tgggtcaacg caaacaacca gaacctctgc ttcattcagc
     5881 aaagtattct cggcgttgac aatacgttgc gtgaaaacca aagaggtgcc acgcattgat
     5941 gttatttcag tctgtatttc gagcatatcg tcgagccgcg caggtacgta atattccacc
     6001 gtcattttac gtaccacaaa ggcaacgcgt tcagccatca gcgcctgctg actgaagtga
     6061 tgatgacgca gcatctctgt gcgtgctctt tcataaaaag cgacgtaact ggcgtggtac
     6121 accacaccac cggcatcggt atcttcatag tagacgcgaa ccggccatcg aaacagcgtt
     6181 gtattcactt tacatcccgg taatgcaaca aaagttagag cttttaaacg ctgctactat
     6241 acgcgcgcga ttgaggtttg ggaatgaggg caagttaagg gagcgtaaaa atttatgggc
     6301 ctggagcggc ccataatttt ttaaagaaaa ttctgattta aaagaagaag aaaatcagcc
     6361 cgacaatgag aacaatatcg gcaagcaatg ggcaaaaaat cccttgccaa agaaccttct
     6421 gcggacgaaa gcccacgccg tgaatcacac cagcacagac ggcccacatc agcaataaac
     6481 catgccagat ttccagttca ctggtcttcg cggcaaaacg agatgggtcc caaaacatac
     6541 atcctgctaa cagaagcgcc atcacgaagg aaagcgcccg cagggggcgc ttgtccatta
     6601 ccgcatacaa ggtcgcgata atcttactca tcagatgtct tcttgaccgg ctttgcctga
     6661 ttcgacgtgt tcaagcgcca gcgcggtgat taccccaaac gaacaggcaa gaagcgttcc
     6721 cagaatccat gcgaaatacc acatttttag ctccttactt agtacagaga gtgggtgtta
     6781 cgttcaatat cttctttggt gatacgaccg aacattttcc agtaacacca agcggtgtag
     6841 agcagaatga tcggtaccag aaccaccgca acccaggtca tgacgttaag cgtcagctgg
     6901 ctggaagttg catcccacat tgtcagactt gcgttcatca tggtgctgga cggcatcaca
     6961 aacgggaaca ttgcgatacc ggctgtcagg atgatgcagg ccagcgtcag ggaggagaac
     7021 acaaacgccc acgcggcttt atccatacgt gcagtcagga tggtcagcag cggaagaacc
     7081 acacccagtg ccggaatagc ccacagaatt ggcgtgttgt tgaagttaac cagccatgcg
     7141 ccagcttcac gaaccacttc tttattcagt gggttagagg ctgcgtaatg gtccattgtc
     7201 gatttcacga cataaccatc gataccgtac atcacccata cgccagccag tgcgaaacag
     7261 accagtgtca ccagcgcagc cacctgagcc gttgcacggg tacgcaggtg cagttcgccc
     7321 acggtacgca tttgcagata ggttgcgccc tgagtaatga tcatccctac gctcaccacg
     7381 cctgccagca ggccgaacgg gttaagcaac tggaagaagt taccggtgta gtacagacgc
     7441 agatattcat caacgttgaa cggtacgccc tgcaacaggt taccgaacgc tacaccaatt
     7501 accagcggcg gaacgaaact accaatgaag atgccccagt cccacatgtt acgccagcgg
     7561 gtttcttcaa tcttggagcg gtagtcaaaa ccgaccggac ggaagaacaa agacgccagc
     7621 acgaggatca tcgccacata gaagccggag aacgcagcgg catagaccat cggccaggca
     7681 gcaaagagtg cgccgcccgc ggtgatcagc caaacctggt taccgtccca gtgtggtgca
     7741 atggagttaa tcataattcg acgctcggtg tcgttacgac cgaggaaacg ggtgagcatg
     7801 cccaccccca tgtcgaaacc gtcagtgact gcaaaaccaa tcagcagaac gccaaccagc
     7861 agccaccaga taaaacgcaa tacttcataa tcgatcattt gacgactcct gtcttagcgt
     7921 gccggctgag tagtcgtgga agactgctca aagtgatagc gaccggtttt caggctgctt
     7981 gggccgaggc gtgcaaactt gaacattaag aacaattctg ccaccaggaa cagggtatac
     8041 aggccgcaaa tcagcaccat tgagaagatg agatcgcctg cggtcagtga cgagttcgcc
     8101 acagctgtcg gcagcacttc accgatagcc cacggttggc ggccatattc agccacgaac
     8161 cagcccgctt ctacagcaat ccacggcagc ggaataccgt acagcgcggc gcgcagaagc
     8221 cattttttct cgccaatgcg gttgcggatg acactccaga aagagagcgc gatgattgcc
     8281 agaagcagga agccacacgc caccatgata cggaacgcaa agtacagcgg cgctacacgc
     8341 gggatggagt ctttggttgc ctgttgaatc tgcgcttcag tcgcatcagc cacgtttggc
     8401 gtatagcgtt tcagcagcag accgtaaccg aggtctttct tcatgctatt gaactggtca
     8461 cgaaccgcct ggtcggtaga accagaacgc agttgttcga gcagagagta cgccttcatc
     8521 ccgttacgaa tgcgttcttc atgctgcacc atcagctctt tcaggccgat aaccggggta
     8581 tccacggaac gcgttgcaat gatgcccagt gcgtaaggga tctgaatcgc aaatttgttc
     8641 gtctcctctt cctgatcagg aatgccgaac agagtaaagg cagcaggcgc aggttgcgtt
     8701 tcccactcgg cttcaatagc agccagtttg gttttctgca cgtcgcccat ttcgtagccg
     8761 gattcatcac ccagaacaat aacagacaga acagcagcca taccgaagct ggcagcgata
     8821 gcaaaggagc gtttagcgaa ggcgaagtca cgacctttca gcatatacca tgcgctgata
     8881 ccgaggatga acatcgcgcc agtcacataa ccagacgcta cagtgtgaac gaatttcacc
     8941 tgagcaaccg ggttaagcac cagctcggag aagctcacca tctccatacg catagtttca
     9001 aagttgaaat cggacgcgat tgggttttgc atccagccgt tcgcaaccag aatccacagt
     9061 gcggacaggt ttgaaccgag cgccaccagc caggtgacac acatatgctg aactttaccc
     9121 agacgatccc aaccgaagaa gaacagacct acaaaggtgg attcgaggaa gaaggccatc
     9181 agaccttcga ttgccagcgg cgcaccgaag atatccccta catagtggga atagtaagac
     9241 cagttagtcc cgaactggaa ctccatggtc agaccggtag ccacacccag agcgaagttg
     9301 ataccaaaca acttgcccca gaacttggtc atatctttat aaatctgttt gccggagagg
     9361 acgtagaccg tttccataat ggccagcagg aacgccatac cgagcgtcag tggcacaaaa
     9421 aggaagtggt acatcgcggt caaggcaaac tgtaagcgcg acagttcgac tatatctaac
     9481 atcatgactc cttgctcatc gcatgaagac tccgagagtg aaccccgtta gaaagggtca
     9541 cacgcatgcc ccaatagaaa tttgtttgaa cactttcatc ataccatgtg aatgcacaca
     9601 aaaacggcgt aaagaaaggt tgcaaacatg ttaataaaaa ctcaaattga tcccacgtat
     9661 atattacgct gcaaaatcct tacaataaac aggtttttgt tgaagcaaat ttgcattttt
     9721 cgacggtgat caatttatag ctaaattacc gcctttcagc caatttgatc gagaacaatt
     9781 tatctctttt tgatgccaat ttccaagact tatacattga taaatatcaa ttcctacaaa
     9841 ttatgttaat catgtttcaa taaggtaact ttagtgacat ttatgtttaa aatgtgtgag
     9901 ttataaggct gaaattagtt tcggttggca atttaaagat aaaaagagac gaagcaaaaa
     9961 cagcacggtt gaccttaatt ctttttttgg tcactggcta atacgacgca gagtttcagg
    10021 attactaata aaggcataaa tccaccccgc cgatattaac atttaattaa cttgtaatta
    10081 tattataatt aactcaccca gggttattat tttgttaaaa ataaagccac atatacgtgg
    10141 cttgagagtg aagataaacg caaaattacc tgatgcgcta cgcttgccag tgtctacatc
    10201 atctctgcaa tatattgaat ttgcctgctt ttgtagaccg aataaagcgt tcatgccgta
    10261 tccggcatga acaaagcgcc ctttgtcaac aatctggccg cgcatatgtg gcttcggttt
    10321 gattgctatt caggcaagcc ggtaactgaa cgtccgtgac tggccgggta aaaaaggccc
    10381 cgatagattt gaaccttgat tttcctcggt ggtaatgtgt tcatccatca tcgtttctga
    10441 gcaagaaatc acctcgcgac tgaaagcaac agtcgcatca caggttgctg attcagccgg
    10501 attaaacagc cgtaaaatca cttcttgtcg gtcttcagct ttcttaagtg cgcttatcag
    10561 gcatcccact gggggcattt tcaacaaact ataactttcc ggcacgttga atccggcttt
    10621 gttgagcttc atcgcatccc atgggatttt gttgtaacac tgtactggag tcagccatgc
    10681 tcgcgcctgc tgagctacac cagcggcggt tggcgtaccg gtataactca ataaacttag
    10741 gcgacaagaa agcagaccac gtagttgtga gtccgggact ggcattttaa ttcccgaagg
    10801 ccgcccaggc cttaaaagca gatcttcttt gcccagtaag cccacgccac gcagcaacgt
    10861 aatggcaaag gttttcttct cttcaccgat gacttcaaat tcacgtaacc cttcgctaaa
    10921 gacagccatg ccgttacgcc cttcctgtaa ggcaacatag ttgagcatat tccataccgg
    10981 aaccggcgct tctttccagc cttcttgctg ccagttgttc attgcactgt cgttcacggg
    11041 gcgcgttagc gaaccaaact gcgtatctgc cagaacactg tcagtgttaa aaggtgtagg
    11101 gaccaggaca cgaaggcgat gatcgtcagc ctggttatca aggttgatat ccacatcaat
    11161 acgcctgctg ttatgactaa gagtgacaac caacaccccc cctactctgc caggcttcat
    11221 gaataatatc gcattgcggt ttcgcgttcg ctgcggtaat tacccactct tcttttgcgg
    11281 gtgaatagtc atattcatca ccatcatcag agctttcttc aatttgcaat acccggtcat
    11341 agcgcacacc gctgtcttta tctaccagtt gcagagaacc atcctcattg agcgcaatct
    11401 gccagaaagc attttccaga atcccttcag cgtcactttt tgccgcaatc acgttgccag
    11461 gctgattcgc ttcaatataa agcgtgcgat agcccataga agggacaatc tggttgatct
    11521 gaatatcaaa ctccataaag ggatcataat tgccataatg aacaatttgc cgatcgatta
    11581 ggccaggatc gatctcacgg gcatggcgaa taaaatacgg tacaggctga ccgcgatcgt
    11641 cttgtagatt aaactggctg gcgcgcagcc gcacagtggt gttaataact tcttcccgcg
    11701 gccagggcat caggttaaac agcacgagtt tgtcggcatc actctgtggc atgttatcgg
    11761 caattttgcg catgtagaaa cgcagcagat tatccgccat gtcttcagcc agttcgaagc
    11821 gggcgacaat ttcgcgatga actttgtcac tgcaacaaca accgatactg tcgtgggcat
    11881 gattttttaa gatctctttc cacattttct ccagcaagcc gtggtggtat tcaaaaccca
    11941 acgtccaggc cagcgttgcc agcggttcca gcagattaac aatcttattt tcaatacgtg
    12001 cgtgggcaat tttgatatcc atgcgcgtgg aaccgatggt gcgatgtacg cgcatatatt
    12061 tgccatcgat aaattcacct ttcagggtgg ccagactttc acgctgcgct tcgatcttct
    12121 caaatacctc ttcaaagcgg ctcatcacaa acttacgttg agggtagatc tcacgtagct
    12181 tatccatcac ttcgaagata ttttgctgca atggcatctg gtcatgacca ttcggcagca
    12241 aaatctcttt ggttacagac gctttttcca gcacgtcaaa ataactgtcg aggcgtttac
    12301 gtaatccgtt ttcgtcggca ggtaagtact taccgatggc gtagcccagc ggcagcacct
    12361 gcgccgtcac ttcgctaccg tcactgcttt gccacaaaaa ctcggtttta tcagtaccgt
    12421 ggcgctccga acatccgcgc cagaacatag tgcgggtaat gccaaatcca ttgtaaatat
    12481 gcggcagttg cccggacatg ccaaaggaat ccggtaaata acctattttc atcggctcgc
    12541 caaacgcgag gcagtcacgc attccgtaca tcagattacg gacgatggat tccgcagaaa
    12601 caatcgtggt atcggtctgg gtataccagg ggccgataat caacttaccg gcttctacct
    12661 gtttcttcac gcggtctttg ttttccggtt tcaccgcgaa ataatcttcg aggatcgccg
    12721 tttgcccgtc gagtacgtaa tatttgtatt cgttgtcctg ttccagtcgg cacaggatct
    12781 cttccatatt attgaccagc agaatgcgtg actcttcggt ggtgaaatac cactcccgat
    12841 cccaatgcat atgcggggtg atgtgaacgc gagataccgc tttcatcgtc gtttcctttt
    12901 tgtttatggc attacgccat cagtcagata gttgccatgc ttaaccgcgt gacgccgcca
    12961 gatcaggaga attgcagtcg agattgcagc ccccaccaat gccgcaccaa accagccaat
    13021 tgccgccata acaccgcccg cgccattatc atgaagtaaa aagagcgaga aaatgccggc
    13081 accgggtgtc gaaaggccga tattcatcgc accgacaata gcgcccgtta ccatagagcc
    13141 cagcacaaac gaaccaataa cccgcagcgg atcttcaatc gccatcggga tcgccccttc
    13201 ggtaatacct gccagcccta acagccaggt ggatttcccg gtctcaattt caaactcttt
    13261 aaacaggcgc ggtgcgagca tcgtggaagc ggttacggta aatgccgaaa ccattttgac
    13321 ggaggcgaaa atggcatacg ggccgtaaac gccgttcgcc attgcgccca ggcagaatgc
    13381 ataagcggct ttgttcactg gccctccaag gtcaaaggaa cacataaaac cgagaatggc
    13441 acccagcaac agcgcgttac ttcctgacag accgttcagc caggcggtaa gcgagttatt
    13501 gatccatgcg acaggttctc ccaccacaaa cagcatcaga ctgcccgctc ccaacgtacc
    13561 gagcaccggg tagagataaa aagtcaggaa tccattgaat ttactgctaa gacgcaagtg
    13621 atttttcacc cagcgcatca agtaaccggc tatcaatccg ccaacgaccg cgccgagaaa
    13681 cccggagccg atcatgttgg cggcaagtcc agccgcaaag cctggcgcta acgccggttt
    13741 atctgccaga gaataggtgg tataggccgc gagcaccggt accatcaaaa ttccgagcag
    13801 cccgccgccc agcttgcggt acatccacag ccaggagttt tcttcattaa acagatcttg
    13861 tagcccgaag atttgcgaca gtaataccgc gaccgccagc accgtgcccc ccgcgacaat
    13921 caacggtacg gcaaaagaga ttccgctcaa cagtgcctgt ttcagctccg ttttgacact
    13981 tttcaccggt tgcgtatctt gctgtacggt acgcgtctca tcgctacgct tgagggtaag
    14041 cgcttgttgg atcaacgctt ctgcatggcg aatcggctcg gcaacaggca ctgaaagcgc
    14101 gggaatgcca ttaaaacgct cactctcctt gatggcgact tcagccgcaa aaatacaggc
    14161 ggttgcacta ttgagttgat ccgccgttaa acgcccttca atgccgttag cgccttgttt
    14221 ttcaacgtaa acatttacgc cgagtttgcg tccggctttt tccagatatt ccgcagccat
    14281 ataggtgtga gcaatacccg ccggacaggc cgttacgcag acgatagttg gcgcgttgga
    14341 aaaagaggca gaaggttgcg tgcctccctt gtcatccagc gccgagagca actcatcagg
    14401 cgtcgtcgcc gactgtatac gcgcccgaat ctcatcatcc gcaaggcgcg tggtcagcgc
    14461 tgtcagcagt tgcatatgcg tagtacccgc ttcattgggg ggaatcgcca gcagcaccac
    14521 taaatcaact gcttccgggc catcaacgcc ttcccactga agcggctcgc tgagtgtggc
    14581 gaccgcaaac gccgcttctt ttaccgcagc agttttgcca tgcggcacag ccaacccttc
    14641 acctaaggcg gtcgggccaa ggctttcacg gcgatacact tcttccagaa attgctcagt
    14701 actggaaatt ttccccagag cagcaagacg ttgagtcaac gcgtggatgg cctcttcacg
    14761 gctggtaaag cgcgcattca gacacaacgc atcgcggtgg gtcagagtcg tcagattcat
    14821 catgatcctc acttgatcgt ttttatagtc gcgccagtgt gcccgataaa acaataccat
    14881 catgtgatca atttcacttg attgaaatta tttgtattgt atttgtatta tttacaggat
    14941 gaatttatcg ggcataatgg ctaaattcat attaatgaga tactaaaaat gggacacaag
    15001 cccttatacc ggcagattgc cgatcgcatt cgtgaacaaa ttgcgcgtgg cgagcttaaa
    15061 cctggcgatg cgttacccac tgaatcagcg ctacaaacag agtttggcgt cagccgggtt
    15121 acggtgcgtc aggctttgcg ccagctagtc gagcagcaga tcctcgaaag cattcagggc
    15181 agcgggactt acgtcaaaga agagcgggtc aattacgata tttttcagtt aaccagtttt
    15241 gatgaaaaac tgtcggaccg tcacgtcgat acacacagtg aaatcctgat atttgaagtg
    15301 attccggctg acgattttct tcagcaacag ctacaaatca ccgcgcagga tcgtgtgtgg
    15361 catgtgaagc gcgtgcgcta tcgcaagcaa aagccaatgg cgctggaaga aacctggatg
    15421 ccgcttgcgt tgttcccgga tctcacctgg caggtcatgg aaaattcgaa atatcacttt
    15481 attgaagaag tgaagaagat ggttatcgat cgtagcgaac aggaaatcat tcctctgatg
    15541 ccaaccgaag agatgagccg tctgcttaat atcagccaga caaagccgat tcttgaaaaa
    15601 gtatcgcgtg gatatttagt tgatggtcgg gtgtttgaat atagccgcaa cgcttttaat
    15661 accgatgact ataaatttac cctgatagcg cagcgaaaat catcgcgata agcacaaaaa
    15721 aggccatcat acgatggcca accatgtcga aaccgacagc ccttcgccat tcctgtagag
    15781 ggaagcggcg agggctattt cttattacag atatttattt cagaacagtt ttcagtgctt
    15841 caccgatatc cgccaggctg cgaacggttt tcacgcctgc ggcttccaga gcagcgaatt
    15901 tctcatccgc agtccctttc ccaccagcaa tgatcgcacc cgcgtggccc atacgtttgc
    15961 ctttcggcgc agtcacacca gcgatgtaac ctacaactgg cttggtaacg tgctctttga
    16021 tgtacgctgc cgcttcttct tcagcgctac cgccgatctc accgatcatc acgatcgctt
    16081 cggtctgcgg atctttttcg aacatttcga ggatgtcgat aaagttagag cctgggatcg
    16141 ggtcaccgcc gataccaaca caggtcgact gaccgaaacc gtaatccgtg gtctgtttaa
    16201 ccgcttcata ggtcagtgta ccggaacggg aaacgatacc cactttaccc ggtttgtgaa
    16261 tgtgacccgg ctggataccg attttgcatt cacccggagt gataacgccc gggcagttcg
    16321 ggccgatcat acgaacgccg gcttcgtcca gtttcacttt cacagtcagc atatccagcg
    16381 tcgggatgcc ttcagtgatg gtgataatca gtttgatgcc tgcatcaata gcttccagaa
    16441 tggagtcttt gcagaacggt gctggtacgt agataacaga agcggtagcg ccagtagcag
    16501 caacagcttc acgcacggtg ttgaacaccg gcaggccgag gtgggtagta ccgcctttac
    16561 ctggggttac gccgccaacc attttagtgc cgtatgcaat ggcctgttct gagtggaaag
    16621 tcccctggct accagtaaag ccctggcaga taaccttggt gtttttatca attaaaatgg
    16681 acattatttc ccctccactg cggcaacaac ctgctgagct gcatccgtca gaccttttgc
    16741 tgcaataata ttcaggccgc tgtcagccag tttcttcgcg ccgagttcgg cgttgttacc
    16801 ttccagacgt accacgaccg gtacgttaac acccacttct gctaccgcgc cgatgatacc
    16861 gtcagcgatc aggtcgcaac gaacgatacc gccgaagatg ttaaccagaa cggctttcac
    16921 tttgtcgtca gagaggatga ttttgaacgc ttcggttaca cgttctttgg ttgcgccgcc
    16981 gccaacgtca aggaagttag ccggttcgcc gccgtgcagt ttaacgatgt ccatcgtacc
    17041 catcgccaga cctgcgccgt taaccataca accgatgtta ccgtccagcg caacgtagtt
    17101 cagttcccac tgtgcagcct gtgcttcacg cggatcttcc tgcgactggt cacgcatttc
    17161 gcgcagatca ggctggcgga acagtgcgtt gccgtcagcg cccagtttgc cgtcgaggca
    17221 aatcagatcg ccctgtttgg tgatgaccag cgggttgatt tcgatcaacg ccaggtcgcg
    17281 ctccaggaaa atggtcgcca ggcccatgaa gattttggtg aactgctgaa ccagtttacc
    17341 ttccagaccc agtttgaacg ccagctcgcg tccctgatac ggcatcgggc cagtcagcgg
    17401 atcaagcgca actttgtgga tcaggtgcgg agtttcttcc gccacttttt cgatttccac
    17461 gccgccttcg gtggaggcca taaagaccac acgacgggaa ctacggtcta caacggcacc
    17521 gagatacagc tctttagcga tatcggtcgc tgcttcaacc agaatctggt taaccggttg
    17581 gccattggca tctgtttgat acgttaccag acgcttgccg agccagtttt ctgcaaaagc
    17641 acggatgtct tctttgctgt ttacaacttt cacaccgccc gctttaccgc ggccaccagc
    17701 gtgaacctga catttcacta cccacgggcc ggcaccgatt tttgaagcgg cttcttctgc
    17761 ttcgcgcgga gtagtacagg cataacccac cggtgctggt aagccatagc gggcaaaaag
    17821 ttgttttgcc tgatattcat gtaagttcat gtgttctgtc catccttcag taatcgttat
    17881 cttttaaacc gtagaccgac accctatcca cgtcggtcta cagtgcaggt gaagcttaaa
    17941 ctactacacg tccagcagca gacgcgtcgg atcttccagc aactctttga tcgttaccag
    18001 gaagcccacg gattcgcgac catcgatcag acggtgatcg taggacagcg ccaggtacat
    18061 catcggcagg atctcaacct gaccattcac cgccatcgga cgatctttga tagcgtgcat
    18121 acccagaatt gcgctctgcg gcgggttgat gatcggcgta gacatcaggg aaccgaacac
    18181 accaccgttg gtgatggtga agttaccacc ggtcagatct tcaaccgtca gcttgccgtc
    18241 acggccttta actgccagct ctttgatttt cttctcgatg tctgccatgc cgagggtatc
    18301 gacatcacgc agtaccggcg tcaccaggcc gcgcggcgtg gaaaccgcca tgctgacgtc
    18361 gaaatagttg tggtaaacca cgtcatcacc gtcgatagaa gcattcactt ccgggtaacg
    18421 tttcagggct tcaaccaccg ctttcacgta gaaggacata aagcccagac ggatgccgtg
    18481 gcgtttttca aacgcttcac cgtactgctt acgcagatcc ataatcggct tcatgttgac
    18541 ttcgttgaac gtggtcagca tggcggtgga gtttttcgct tccagcagac gctctgccac
    18601 acgcttacgc aggcgagtca tcgggacacg tttttcacta cgtgcagcca gagccggttg
    18661 cgccgccgga gcagccgctg ccggagcaga ctctttcgcc ggggctttcg ccagatgttt
    18721 ttccacatct tcacgagtca gacgaccacc cacaccggtg cctttaatgg cgctggcgtc
    18781 gagattgtgt tcagccagca ggcgacggat cgccgggctt aacgcatcgt tgttttgctc
    18841 ttccagagac gcctgctggc gttgcgccgg agtggacgct ttctcttcag atttggcgct
    18901 ggtttcttta ccggcgctgt tgccttcacg caggcgacca aggatctgac gagacgttac
    18961 cgttgtacct tcatcttcca gaaccgcatc cagaatgccg tctgctgatg ccggtacttc
    19021 cagtaccact ttgtcagttt cgatttctac cagcacttca tcacgtacga ctgcgtcgcc
    19081 gggtttttta tgccaggttg cgacggtggc atcggctacg gattcaggca ggtcagggac
    19141 cagaatatct acgctactca ttgtgtatcc tttatttatt cgacgttcag cgcgtcatta
    19201 accagatctt gttgctgttt ctggtgaacg gacatatacc ctaccgccgg agaggcggag
    19261 gccgtgcggc ctgcataacg cagagaagcc ccaaacggaa tcacttcacg gaaatgatgc
    19321 tggctgcagt accatgcgcc ctggttgagc ggctcttcct ggcaccagac aaaatccttg
    19381 acgtgagcaa actgctgcaa cacttcctgc atcgctttat gcgggaacgg gtagagttgc
    19441 tcgatacgca caatggcgac atcgtgttga ttgttcttac gacgctgttc cagcaggtcg
    19501 taataaacct taccagaaca catcactacg cgcttcacgc ccttcggatc aagctcgtcg
    19561 atttcaccga tggctggcag gaaggtgccg ttcgccagtt cttcgaggct ggaaaccgcc
    19621 agcggatgac gcagcaggga tttcggcgac atcactacca gcggacgacg catcccgcgc
    19681 agcgcctgac gacgcagcat gtggtaaacc tgtgccgggg tagacggaac gcaaacctgc
    19741 atgttttgct cagcacaaag ttgcagataa cgttccagac gcgcggagga gtgctccggc
    19801 ccctgccctt cgtaaccgtg cggcagcaac atcaccagac cacacatccg gccccatttc
    19861 tgttcgccag aagagatgaa ctggtcgata accacctgcg caccgttggc gaagtcaccg
    19921 aactgcgctt cccagatggt cagagtgcgt ggttctgcgg tggcataacc gtattcaaac
    19981 gccagcactg cttcttcaga cagtacggag tcccagacgc ggaacgcgcc ctgcccgtta
    20041 tggatgtgtt gcaacggcgt gtaagtggaa ccgttagact ggttgtggat caccgcgtgg
    20101 cggtggaaga aggtaccgcg accggagtct tcacccgaca ggcgaaccgg aatgccttcg
    20161 tcaaccagcg tggcgtaagc gaggttttcc gcaccgcccc agtcgaacag tttctcaccg
    20221 gcagccatcg cctggcgatc gccataaatc ttggcaacgc gagactgcat ttcaactgct
    20281 tccggcaccg tgctgatgcg tttcgccagc tcctgcaggc gcttcatctc aactttgttc
    20341 gggtactctt cgtcccattc gtggttgagg tacggcgacc aggtgaaaga gtgcatgttc
    20401 atcggacgcc actctgccac tacgcaatcg ccagcatcca gcgcatcgcg gtacaggtta
    20461 accatctcgg tggcatcttc cagcgtcgcc actttttcct gctccagctt gtcagcgtag
    20521 attttgcgcg gtgtcggatg ttttttgatt ttctgataca tcagcggctg ggttgcgctc
    20581 ggctcgtcgg cttcgttgtg gccgtgacgg cggtagcaca ccaggtcgat gaagacatca
    20641 cgtttaaagg tgttacggaa atcgagcgcc agacgggtca caaaggcaac ggcttccgga
    20701 tcatccgcgt taacgtggaa aatcggtgcc tgaaccatct taccgatatc agtacagtac
    20761 ggtgtagaac gagcatccag tggattagag gtggtgaaac caacctggtt gttgataacg
    20821 atacgtaccg taccgccaac ttcataacca cgcgctttcg acatgttcag agtttcctga
    20881 accacgccct gcccggtcac tgcggcgtca ccgtggatgg tgattggcag cactttgttg
    20941 ctgctcggct catcaagtct gtccagacgg gcacgaacag aaccgataac taccgggctt
    21001 acaatctcaa ggtgagacgg gttaaacgcc agcgccaggt gcaccaggcc gccatcggtc
    21061 tggaagtcag acgagaagcc catgtggtat ttcacgtcac ccgtgccgag gtgttcttta
    21121 tgtttaccgg caaactcgtc gaacaagtct tgcggttttt tacccagcac gttcaccagc
    21181 acgttcagac gaccacggtg cgccatccca agaaccactt cgcgggtgcc gctgttgcca
    21241 gcgtggcgga tcatctcttt aagcatcggg attaacgcgt caccgccttc cagcgagaag
    21301 cgttttgcgc cagggaattt tgcgccgagg taacgttcaa ggccttcagc ggcggtcagt
    21361 tcgcttaaga agcgtttttt ctcttcgcta ttgaaagtcg cgcgaccaga ctcgatacgc
    21421 tgttggatcc agcgtttttc ttcggtgctg gtaatgtgca tatactcggc accaatcggg
    21481 ccacagtagg tttgcttgag ggcttccagc agctcgccga gtttcatgtt ttctttgccg
    21541 ctggcaaatg aaccgacgtt gaaggtctcc tggaagtctg cttcggtcag atcgtggaaa
    21601 gacggatcca gatcggccac tttatcttgc tgccacagtc ccagcggatc gagattcgca
    21661 tgctggtgac cacggaagcg gtatgcgtta atgagctgca ggactttaac ctgcttcaca
    21721 ttggtgtcag ggtcggagat cgttgaagag taacgtgaag cgtctttcgc caggcggcgg
    21781 aaatattcac gcgtttgaga gtggaattga tccggtttga ctcccgtacc aggtaactgc
    21841 tggaacgtcg aacgccagtt agcgtcaacc gagtcaggat cggttaagaa gtcttcatag
    21901 agctgttcta tccagctctg gtttgcgcca gagaggtaag aagagtccaa ccaggctttc
    21961 aaagcgctgt tctgcatcgt gatcccttaa gcatcttttt tatgcttact tcgccgtgga
    22021 tactaccacg cacagtgatg tgcgtgccgg ggttcacttg cgagctcttg tctatgtagt
    22081 ggctcgcgaa ggaaccttta aaaactgtct tagtgtcagg ggcagttttt aaagatttcc
    22141 tgcatttctt tgttacgcct gatgcgctgc gcttatcagg cctacgggta atgacttgta
    22201 ggccggataa ggcgttcacg ccgcatccgg cactggttgc ctgatgcgac gcttgcgcgt
    22261 cttatcaggc ctacggttta cgcattacgt tgcaacaaca tcgacttaat atggccgatg
    22321 gcgcgcgtcg ggttcagccc cttcggacat acactgacgc agttcatgat gctgtgacag
    22381 cggaatacgc tgaatgcatc gctcaaaccg tcgaggcggc tgtcagtctc ggtatcacgg
    22441 ctatcgatca ggaaacgata tgccgctaac aagcctgccg ggccgataaa cttatcggga
    22501 ttccaccaga aagacggaca agaggttgaa caacatgcgc agagaataca ttcatacagc
    22561 ccgtcgagtt tttcgcgctg ctctggcatc tgtaaatgct cgcgagctgg cggattttgt
    22621 ccattattca acaggtaagg cttaattttc tcatattgcg catagaattg tcccatgtct
    22681 accaccaaat cgcggatcac cggtaaacct ggcagcgggc gaatcacaat cttcttgccc
    22741 ggctggttga gtgccgaaat cggggtaata caggccagac cattcttgcc gttcatgttc
    22801 agaccgtcgg aaccgcacac accttcacgg caggagcggc ggaacgacag gctgggatct
    22861 ttctctttca gctggataag cgcatccagc agcatcatgt cgcgaccttc ttccgcttcc
    22921 agggggtaat cctgcatacg cggagcatca tcaacatccg ggttatagcg ataaattgaa
    22981 aactcgagtc tcattttcct gtctccgcat tagtaagtac gaatcttcgg cgggaatgcc
    23041 gggcgcagtt tcggttccat gttgacgctt cggcgcgtca tggattccga ctctggcaga
    23101 tacagggagt ggcacagcca gttttcatca tcacgatccg ggaagtcgaa gcggctatgt
    23161 gcgccacggc tttcggtacg gaagttggca gaaacagccg ttgcatacgc cgtttccatc
    23221 aggttatcca gttccaagca ctcaacgcgc tgggtgttga actcgctgga agtgtcatcc
    23281 agacgggcat ttttcagacg ctcgcggatc actttcaact gctcaagccc tttcgccatc
    23341 gcatcacctt cacggaagac cgagaagtta tgctgcatac attcttgcag cgctttacga
    23401 atcgccaccg gatcttcacc gttacgatta ttgttccagc ggtgcagacg atccagagat
    23461 gcttcaacat cagactcgct ggcatcgcgc aatgcgccct gttcggcgat agactcttgc
    23521 agatgcagac ctgccgcacg accaaagacc accaggtcca gcagcgagtt gccgcccaga
    23581 cggttagcgc cgtgtaccga tacacaagcg atttcaccaa cggcaaacag ccccggaaca
    23641 accacatctt cgcctttctc gttcaccgtc agcgcctgac cggtcacttt ggtcggaata
    23701 ccgcccatca tgtagtgaca ggttgggata accggaatcg gctctttcac cggatcgacg
    23761 tgagcaaagg tacgggaaag ctccaggata cccggcagac gggattcgag aacttcttta
    23821 cccaggtgat cgagtttcag tttcgcgtgt ggcccccacg gaccatcaca gccgcgacct
    23881 tcacggattt caatcatgat ggaacgcgcc accacatcac ggcccgccag gtctttggcg
    23941 ttcggcgcat aacgctccat aaagcgttcg ccatgtttgt tcagcagata accgccttca
    24001 ccacggcaac cttcggtgac cagtacgccc gcaccggcaa tgccggtcgg gtggaattgc
    24061 cacatttcca tatcctgcac cggtacgcct gcgcggatag ccatgccgac accgtcgcca
    24121 gtgttaatgt gggcgttagt ggtggactga taaatacgcc ctgccccgcc agtcgccagc
    24181 acggtagcgc gggctttgaa ataaaccact tcaccggttt cgatgcacag tgcggtacaa
    24241 cccaccaccg cgccatcctg gtttttcacc agatccagcg cataccactc ggagaaaatg
    24301 gtggtgtggt ttttcaggtt ctgctgataa agcgtgtgca acagtgcgtg accggtacgg
    24361 tcagccgccg ccgcagtgcg tgccgcctgc tcgccgccga agtttttcga ctgaccgcca
    24421 aacggacgtt gatagatacg accatcatca agacgcgaga acggcaggcc catgtgttcg
    24481 agttccagaa tcgcttccgg cccggtttta cacatatatt caatcgcgtc ctggtcaccg
    24541 atatagtccg acccttttac ggtgtcgtac atatgccatt cccagttatc ttcatgggta
    24601 ttacccagcg caacggtaat gccgccttgc gcagaaacgg tatgggaacg ggtcgggaag
    24661 actttagaga gcagcgcaca ggtctggccg ctctgggaaa tttgcagcgc cgcgcgcata
    24721 cctgcgccac cggcaccaat cacaactgca tcaaattctc tgactggcaa tttcatcaca
    24781 caccccacac cacaacgaat ccataaatca cgtaaaccac cagtgcaacg acaatcacca
    24841 gttgcagcat caggcgcaat gccagcggtt taacgtagtc ggtcaacacc tgccacatgc
    24901 cgatccaggc atgaatcaag atagaaaaca gcgccagcag ggtgaacact ttggtgaacg
    24961 cagaggcgaa gaaaccgatc cagacttcat atgtcagttc gccactggtg gcgaaaaaac
    25021 cgaccatata aatgatgtag agcgtcagga cgatagcggt agcgcgaacg aggatgaaat
    25081 catgtacgcc attgcgtcct aatgcggagg cgttgcttac catacgagga ctcctgcgag
    25141 aagtgaaagc acgacagtaa taacaaagga gattttggcg gagcgtttac ccgcttcgaa
    25201 tgtttcttcc agatagccaa aatccatcat catgtggcga atacctacga cgacgtgata
    25261 cgccagagcg gtaaggatgc cccacatgat aaatttgacg aagaagctgc ccataatcgc
    25321 ggaagcttgc tcgaaacctt caggggaaga gaggctggta cccagaagcc acagcaggat
    25381 gcccactgca acaaaggtga tcacaccgga aacgcgatgg agaatggacg ctatcgccgt
    25441 gatggggaac cggatggtct gtaggtccag attaacaggt ctttgttttt tcacatttct
    25501 tatcatgaat aacgcccaca tgctgttctt attattccct ggggactacg ggcacagagg
    25561 ttaactttct gttacctgga gacgtcggga tttccttcct ccggtctgct tgcgggtcag
    25621 acagcgtcct ttctataact gcgcgtcatg caaaacactg cttccagatg cgaaaacgac
    25681 acgttacaac gctgggtggc tcgggattgc agggtgttcc ggagacctgg cggcagtata
    25741 ggctgttcac aaaatcatta caattaacct acatatagtt tgtcgggttt tatcctgaac
    25801 agtgatccag gtcacgataa caacatttat ttaattttta atcatctaat ttgacaatca
    25861 ttcaacaaag ttgttacaaa cattgccagg aaaagcatat aatgcgtaaa agttatgaag
    25921 tcggtatttc acctaagatt aacttatgta acagtgtgga agtattgacc aattcattcg
    25981 ggacagttat tagtggtaga caagtttaat aattcggatt gctaagtact tgattcgcca
    26041 tttattcgtc atcaatggat cctttacctg caagcgccca gagctctgta cccaggtttt
    26101 cccctctttc acagagcggc gagccaaata acaaacgggt aaagccaggt tgatgtgcga
    26161 aggcaaattt aagttccggc agtcttacgc aataaggcgc taaggagacc ttaaatggct
    26221 gatacaaaag caaaactcac cctcaacggg gacacagctg ttgaactgga tgtgctgaaa
    26281 ggcacgctgg gtcaagatgt tattgatatc cgtactctcg gttcaaaagg tgtgttcacc
    26341 tttgacccag gcttcacttc aaccgcatcc tgcgaatcta aaattacttt tattgatggt
    26401 gatgaaggta ttttgctgca ccgcggtttc ccgatcgatc agctggcgac cgattctaac
    26461 tacctggaag tttgttacat cctgctgaat ggtgaaaaac cgactcagga acagtatgac
    26521 gaatttaaaa ctacggtgac ccgtcatacc atgatccacg agcagattac ccgtctgttc
    26581 catgctttcc gtcgcgactc gcatccaatg gcagtcatgt gtggtattac cggcgcgctg
    26641 gcggcgttct atcacgactc gctggatgtt aacaatcctc gtcaccgtga aattgccgcg
    26701 ttccgcctgc tgtcgaaaat gccgaccatg gccgcgatgt gttacaagta ttccattggt
    26761 cagccatttg tttatccgcg caacgatctc tcctatgccg gtaacttcct gaatatgatg
    26821 ttctccacgc cgtgcgaacc gtatgaagtt aatccgattc tggaacgtgc tatggaccgt
    26881 attctgatcc tgcacgctga ccatgaacag aacgcctcta cctccaccgt gcgtaccgct
    26941 ggctcttcgg gtgcgaaccc gtttgcctgt atcgcagcag gtattgcttc actgtgggga
    27001 cctgcgcacg gtggtgctaa cgaagcggcg ctgaaaatgc tggaagaaat tagctccgtt
    27061 aaacacattc cggaatttgt tcgtcgtgcg aaagataaaa atgattcttt ccgcctgatg
    27121 ggcttcggtc accgcgtgta caaaaattac gacccgcgcg ccaccgtaat gcgtgaaacc
    27181 tgccatgaag ttctgaaaga gctgggcacc aaagatgacc tgctggaagt ggctatggag
    27241 ctggaaaaca tcgcgctgaa cgacccgtac tttatcgaga agaaactgta cccgaacgtc
    27301 gatttctact ctggtatcat cctgaaagcg atgggtattc cgtcttccat gttcaccgtc
    27361 attttcgcaa tggcacgtac cgttggctgg atcgcccact ggagcgaaat gcacagtgac
    27421 ggtatgaaga ttgcccgtcc gcgtcagctg tatacaggat atgaaaaacg cgactttaaa
    27481 agcgatatca agcgttaatg gttgattgct aagttgtaaa tattttaacc cgccgttcaa
    27541 atggcgggtt aatttttata tgcccaaaca caaaaaattg taaaaataaa atccactatc
    27601 ggacattcat atattctaaa aaaaatatat cggtataaat tataaacaaa tcgatgattt
    27661 ttaatcatct atcttcctct cagttacatg aataaaccac caaatatagc gcacccctat
    27721 aatttccaat actttattca tcaaacattt aattcatacc atcaattaat tacttttaca
    27781 tttaataaaa ttaataatat ttttaagaaa aactaaaaac attcgacaat caaataagat
    27841 attcttgcac cgatatactt ttttcttttc tgttaaagga ctcagttatg tttaaaggac
    27901 aaaaaacatt ggccgcactg gccgtatctc tgctgttcac agcacctgtt tatgcggctg
    27961 atgaaggatc cggtgaaatt cactttaaag gtgaagttat tgaagcaccg tgtgaaatac
    28021 atcaggatga tattgataaa gaggttgaac tcggtcaggt gaccaccagc cacattaatc
    28081 agtcacatca cagcgatgcc gttgctgtcg acctgcgctt agtcaactgt gatctggaaa
    28141 actccagcaa cggttccggt ggcaagattt ctaaagttgc agtcaccttt gatagctcag
    28201 cgaaaaccac cggcgcagat ccgattctca acaacaccag cacgggtgaa gccaccggcg
    28261 tcggcgtacg tttaatgaat aaagatcaaa gcaatatcgt tttaggcacc gctactccag
    28321 atatcgacct ggctccgacc tccagcgaac aaacgctgaa tttctttgcc tggatggaac
    28381 aaattgatca ggctacacct gtaacgccag gcgcagttac ggcgaatgca acctacgtgc
    28441 tggattataa ataagtatta ctttggcggc gctatcgccg cctttcataa aatatttatc
    28501 aggtatggac accgtgaata tttatcgact ctcttttgta tcctgcctgg tcgtggcgat
    28561 gccttgcgca ttggcggtcg aattcaacct taatgttctc gataaatcga tgcgcgaccg
    28621 cattgatatt tcattattaa aggaaaaagg agtcattgct cccggtgagt attttgttag
    28681 cgttgcggta aataataacc aaatcagtaa cgggcaaaag attaactggc acaaaaatga
    28741 cgataaaacc attccgtgca tcaatgattt actggtcgat aaatttggct taaaacctga
    28801 agtccgtcag tcgttaccat tgataaatca gtgcgtcgat tttagctccc gacctgaaat
    28861 gctcttcaat ttcgatcaag ccaatcagca actgaatatt agtattccgc aagcctggct
    28921 ggcgtggcac tcagaaaact ggaccccacc ctccacatgg aaagaaggtg tcgccggtat
    28981 cctgatggat tacaacttgt ttgccagcag ctaccgccca caggacggca gcagcagcac
    29041 taacctgaac gcctacggta ccaccggaat taacgccggg gcatggcgct tacgtagtga
    29101 ttatcagttg aatcagactg atagcgatga taaccatgaa cagtcaggcg gaatatcgcg
    29161 cacctatctt tttcgtccat taccgcaatt aggctctaaa ttaaccctcg gcgaaacgga
    29221 ttttagttcc aatattttcg acggtttttc ttataccggc gcggcactgg caagtgacga
    29281 gcgaatgttg ccatgggaac tacgcggcta cgccccacaa attagcggta ttgcacagac
    29341 caatgccacg gtgacgatca gtcaatcagg ccgcgtcatt taccagaaaa aagtcccacc
    29401 aggcccattt atcattgacg accttaatca gtctgttcag ggcacactgg atgtcaaagt
    29461 gacggaagaa gatggtcggg tgaacaattt ccaggtttcg gcagcatcga cgcccttcct
    29521 gactcgtcag ggacaggttc gctataaact ggccgcgggt cagccacggc cctccatgtc
    29581 acatcaaact gaaaatgaaa ccttttttag caatgaagtt tcctggggga tgctgtcaaa
    29641 cacctcgctg tacggcggcc tgctgctttc tggtgatgac taccattctg ccgcaatggg
    29701 tattgggcaa aatatgctgt ggcttggtgc gctgtcgttt gatgtcacgt gggccagtag
    29761 ccattttgat actcagcagg acgagcgggg cttaagctac cgttttaatt acagcaaaca
    29821 agtggatgcc actaacagca cgatttccct cgccgcttat cgtttctccg atcgtcattt
    29881 tcacagctac gccaactatc tggatcacaa atacaacgac agcgatgcgc aggacgaaaa
    29941 acagacgatc agcttattgt gggccaaccg attaccccac taaacctcaa tctttacgcc
    30001 aacctgctac atcaaacctg gtggaatgca gacgcctcca cgaccgccaa catcacggca
    30061 ggttttaatg ttgatattgg tgactggaga gatatctcta tttcgacgtc attcaataca
    30121 acccattacg aagataaaga tcacgacaac cagatttacc tgtcgatttc gctccccttc
    30181 ggtaacggtg gtcgggttgg ctatgacatg caaaacagta gccacagcac cacacaccgc
    30241 atgtcgtgga acgatacgct ggatgaacgt aatagctggg gcatgtctgc cggactgcaa
    30301 tccgaccgtc ctgacaatgg agcccaggtg agcggtaact atcagcacct gagttcagcg
    30361 ggtgagtggg atatttctgg tacctatgcc gccaatgatt acagttccgt cagcagcagc
    30421 tggagcggtt ctttcaccgc aacccaatat ggtgcagcgt ttcatcgccg cagctccacc
    30481 aatgaaccac gcctgatggt cagcaccgat ggcgtggcag atattccggt tcagggcaat
    30541 ctcgactaca ccaaccattt tggcattgcg gtggtgccgt tgatttccag ttaccagcct
    30601 tccaccgtgg cggtgaacat gaatgactta cccgacggcg taacagttgc agaaaacgtt
    30661 atcaaagaaa cgtggattga aggcgcgata ggttacaaat cactggcttc ccgttccggt
    30721 aaagacgtta acgtcatcat tcgcaacgcc agcggtcagt ttcctcccct cggagcggat
    30781 atccgccagg atgacagcgg cattagcgtg gggatggttg gcgaggaagg acatgcctgg
    30841 ttaagcggag tcgctgaaaa tcaaaagttt accgtggtct ggggtgatag ccagcattgc
    30901 tcgctccatc ttcctgaaca tatggaagac accgcaaatc gcctgatttt accttgtcat
    30961 taattaagga aatgaaaatg acatttatga aaggactacc ccttttgttg ttggttgcca
    31021 gtctgtgcag ccacgctgca ctacaacccg atcgcactcg tattgtgttt aacgctaatg
    31081 ataaagctac cagtctgcgg gtggataatc gtagcgataa actcccctac ctcgcttatt
    31141 cctggctcga aaatgaaaaa ggggagaaaa gcgatgatct tctggtcgcc ctgccgccga
    31201 tacaacgtct ggaaccgaaa gcaacaacgc aggtacggat agtaaagcaa gcctcgacca
    31261 caaaattacc gggcgatcgc gaaacgctat ttttctacaa tatgcgtgaa attcctcccg
    31321 ccccggaaaa aaacagcgac catgccgttc tgcaagtcgc cattcagagt cgtatcaagg
    31381 tgttctggcg accggctgca ttacgcaaga aagcaggaga aaaggtcgaa ctgcagttac
    31441 aggtcagcca gcagggcaac caactgacac tgaaaaatcc taccgcgtat tatctgacca
    31501 ttgcctacct gggacgaaat gaaaaaggcg ttctccccgg cttcaaaacc gtgatggttg
    31561 cgccttttag tacggtcaac acaaacaccg gaaattatag cggcagccag ttttatctcg
    31621 gttatatgga tgattacggc gcgttacgca tgacgacgct caactgcagc ggacaatgcc
    31681 atttacaagc cgtggaggcg aagaaatgag tgctggcaag ggagtgttgc tcgtcatctg
    31741 tctgttattt ctgccgttga agtctgcgct tgccctgaac tgctactttg gttcatccgg
    31801 tggctcggta gaaaaatcag aagcgattca accttttgct gtaccaggca atgccaaact
    31861 cggcgataag atttgggaat ctgacgatat taaaattccc gtctattgtg acaacaatac
    31921 caacggcaat tttgaaagtg agcatgttta tgcctgggta aacccctacc cgggggtgca
    31981 ggatcgctat tatcaactcg gcgtaactta caatggcgtt gattatgacg ccaaccaggg
    32041 aaaaagccgc atcgacacta accagtgtat tgacagtaag aacattgaca tttatacccc
    32101 tgagcagatt atcgcgatgg gatggcagaa caaaatttgc tcgggtgatc ccgctaatat
    32161 tcacatgtcg cgtacctttc tcgctcgcat gcggttatac gtcaaaatac gagaaatgcc
    32221 gccgcatgat tatcaaagta cgcttagcga ctatatcgtc gtgcaatttg acggtgccgg
    32281 tagcgttaac gaagacccta ctgcccaaaa cctgaaatat catattactg gtctggaaaa
    32341 cattcgcgtg ctggattgca gcgtcaattt ttccatttca ccggaaacac aggtgattga
    32401 ttttggtaaa tttaatttgc tggatattcg tcgccacaca atgtcgaaaa cattcagtat
    32461 taagacgaca aaatcacaaa atgaccaatg caccgacgga tttaaagtca gttcctcctt
    32521 ttataccgaa gaaacgttgg ttgaagaaga taaagcattg ttgattggta atggtctgaa
    32581 gctacgtttg ctggatgaaa acgcctcgcc ttataccttc aataaatatg ctgaatacgc
    32641 cgatttcacc agcgacatgt tggtctacga aaaaacctat acagctgaac tttcgtccat
    32701 tgcaggtacc cccatcgagg ctgggccctt cgatacagtg gtgcttttta aaattaacta
    32761 taactgattt gccatgatgc cctttgcagc cggaaagggc atctctttca gaaaattgct
    32821 gattttcccc ttttccttga ttgctgttat tttttcctgg cccggctttc tttatgctgt
    32881 atcgatacac caaagagaaa ataatgaggg agcgaaggat gccagttttg cagtggggaa
    32941 tgttatgtgt gctatcactt ctcctttcta ttggtttcct cgcggtacat ctcccggcgg
    33001 cgctattact tgggccgatg atcgccggga tcatcttcag tatgcgcgga attactctgc
    33061 aacttccccg ctccgctttt ctcgccgcgc aggccattct tggctgcatg attgcgcaaa
    33121 acctcactgg ttcgattctc accacgctgg ccgccaactg gccgatcgtg cttgctattt
    33181 tactggtgac gctgctttcc agcgccatcg tgggttggtt attggtgcgc tatagctcac
    33241 tgcctggaaa taccggtgcc tggggttctt ctccaggcgg cgcagcggca atggtcgcta
    33301 tggcgcaaga ttacggcgca gatattcgcc tggttgcatt tatgcaatat ctgcgagtgc
    33361 tgttcgtcgc gggggccgct gttctggtca cccgcatgat gctgggtgat aacgctgaag
    33421 cggttaacca gcaaatcgtc tggttcccgc cagtgagcat taatctcctg cttacgattt
    33481 tgctcgcagt cgttgccggt acggcgggat gcatgctgcg tcttccttcc ggcacgatgc
    33541 tcatcccgat gctggcgggc gcagtgctcc agtctggtca gctcatcacc atcgaactac
    33601 cggaatggct gctggcgatg gcgtatatgg caattggctg gcggattggt cttggtttcg
    33661 ataaacaaat cttactgcgg gcactacgcc cgctaccgca aatcctgctg tcgatttttg
    33721 ctctgctggc tatttgtgcg ggtatggcgt gggggctgac ccggtttatg cacattgatt
    33781 ttatgactgc ctacctcgcc accagtcccg gcgggcttga taccgtggcg gtcatcgccg
    33841 cagggagcaa tgccgatatg gcgcttatta tggcgatgca aaccctgcgc ctgttcagta
    33901 ttttgctgac ggggccagcc attgcgcggt ttatttcaac ctatgcgccg aagcggtcgg
    33961 cctagtgctg gcagccaggg caccagtaaa acgggcgaga tgacagcgtg gttttctcaa
    34021 tgatgctgcc acagcgttcg cacggttcgc catcacgatg aaaaacctta aagcgaaaca
    34081 gcgccccatg atgcttattc tcatccacca gccccctcgt agcgtaggaa aatcgaggaa
    34141 tatccagtaa cgcgtgtgcg agtgcatcca gttgcgccgc attgagatct ttcgctttat
    34201 gatttccagt caacccaacc tgccagagga tctccacccg caaataattg ccaagcccag
    34261 ccagaaacgc ctgatcgagc agtaatcctg caaactgacg gttacgaaag cgcggtgaca
    34321 ataaccgttc ctttaccacc tccggcgtca gattcggatc cagcacatcg ggaccaacgc
    34381 gttgtaaaaa cggatgcgtg gtcagttgtt ccggcctcaa catctcaata tccgaggcgc
    34441 tataaagcag aatggttttg tcagccgttt gcagttttac ccgcaatact cgcgtggtct
    34501 gcggctcttc gccggtatca accacgcgcc agacgccgta aagctgatta tggctgtaga
    34561 gcgttaagtc gttggaaaaa tgagttaaca acgccttacc acgcgtttcc acatgggtaa
    34621 cgtgttgacc gataagttgt gattgataag tttttaactg cgggaaggca aaccagacat
    34681 cagttagtgg tttgcctttg atcgccgcct ccaggttatc cgctgcacgg cggatctccg
    34741 ggccttcagg catggtgata tccttttttc attctttatt aatcggggtt atgctgcgac
    34801 aacaatctcc ttttcggcaa atgtagagcg tagtcggcgg gcgaacgcca gtgcgtgctc
    34861 gccgtcgcca tgcaggcaga ctgtttgcgc cgtgaccgtt gcccattcgc cggtgatact
    34921 tttgactctg ccgtgttgca ccatctctag cgtttgcgcc aacgcctgtt cttcgttttc
    34981 aatcaacgcg cctcgctgac ttcgcggcac cagcgagccg tcagcctgat aaccgcgatc
    35041 ggcaaacact tcctcgcgcg ttgtcaggcc atattgcttg cctgcacgaa tcagctcgct
    35101 tcctgccagc ccaacgagaa tcaatgccgg atcgcaagcg tataccgctc tggcgatggc
    35161 gtctgccagt tgtgcttctt tcgccgcctg gttgtacaac atgccgtgcg gtttgacatg
    35221 acgcattacg ccgccttgcg cacgggcaat cgttgccagc gcgccaattt gatacagcgt
    35281 ctgggcgtaa acggtttctg gcggcagctg catggcgctg cgaccaaaat tttccctgtc
    35341 gggaaaactc gggtgagcgc caatcgcgac accatttttt attgcttcac gcacgcaagc
    35401 ctgcatgatt tgcgcatcgc ctgcatgaaa tccacaggca atattggcag aggaaaccag
    35461 cgttaatagc tctgcgtcgc tggcgcagcc ttcgcccaga tcggcgttca ggtcaatttt
    35521 cattgtgcag ccgccacact aattgttcga aataacgttg ctgatcttgc cgcgctttca
    35581 gtgcctcttc cagtgaacac tggacaaaat gaatcggctg accgagcgga atttgcgcca
    35641 gatggtacat atcagcctca atgatacagg caatacgcgg gtaaccatcg gtggtctgtg
    35701 cgtcgttcat caacacaatc ggctgcccgt tatgtggcac ctgcaccacg cccggtaaca
    35761 aaccgtgaga taacagttcg cgatcggtgg tgcgttttaa aatttgcccc tgtaagcgat
    35821 agcccatgcg gttactttgc gagctaagct gccagggcga acgccagaat gcatcctgcg
    35881 aggcgcgatc gaactcatga tattccggcc ccggcaaggc gcgaatgcgg ttgccccaca
    35941 gcagctgttt aacgccctgc gcttccataa aatcacgctt cgatttgcca atcgggagtc
    36001 ggtcaccatc cttcagtaaa cggccttcca gcccgccaat ccccactttg agatcggtgc
    36061 tgcatgaccc cattaccggc ggaacatcaa taccacccgc gaccgccaga taactgcgca
    36121 tcccgtgctg cgggcgttta agcgttaaac gctggcctgc tttcatcggc aatcgccagc
    36181 cggtccagac ggcattatca tccagccgcg cttcgcaacc ggcacccgtc agagcaaacc
    36241 acccatcagt ttcgaactca accgttaact gaccgagcgt gatctccagc gcgggggcat
    36301 tggcgtcatt acccaccagc aggttagcaa tgcgtaacgc aggcatatcc agtgcgccgc
    36361 agtggctgat tcccgactgg cgaaaaccgt gacgaccgcc atcctgcaca gtggtataca
    36421 tgcccgcacg aataatcttc agcatactcc ctccttctgc ggtacaaagc gcacgctgtc
    36481 tcccggacgt aataagatgg gttcgtcacg cgccggatca aacaggctga gtgaggtatg
    36541 accaatcaac tgccagccac ccggcgttgc cagcggataa acaccagtct gcggcccgcc
    36601 gatcccgaca gaacctgccg gaacgagcaa gcgcggttca gcgcgccgtg gcgtgtgtag
    36661 ttgttccggc aaactcccga gatacgggaa gcccggttga aaacctaaaa accagaccac
    36721 gtattccacg gaggagtgca attcaacaac ctgtttttcg ctaaacccgc aatgcgccgc
    36781 gaccaccgcc aaatccggtc cgcctgcacc accgtaaacc accggaattt caataaagcg
    36841 agactcaggt tccagggcct cgctctcctc ccaccagcgt tgcaaacgct ctatggcatc
    36901 cagcgccagc gactcaggat tacgcaaaat caccgtgata ttgttcatgc cgggaatggc
    36961 ttcaaccaca ttcggcatat ccaccagacg ctgcgccagt cgccagatcc gtttctggct
    37021 agccagcgtc accggcggtt ccagttccag cactaccgcc gtttcaccta tcagataaca
    37081 acgcgctcgt tgcactttcg atccctctga ttattcgtta tgcaggatta ggaatatcaa
    37141 taaaggtcac atcaagatcg gtattttcat tcagccactc gctcaatgcg cgaataccac
    37201 cacgttcagt ggcatggtga cctgcagcat aaaaatgcaa cccctgctca cgggctgaat
    37261 gaatggtctg ttcagaaact tcgccagtaa taaaagcatc cacgccaaaa cgcgcggcgc
    37321 tatcgataaa actttgcccg ccgcccgtgc accaggcgac gcgctggacc acctcaggtc
    37381 cggtatcgcc acaccataac ggcttacgtc ccagacgcgc ttcaatccag gaggctagtt
    37441 ccagtcccgg cacaggcatg gtcagctctc cccacggcac caacggctca atttcgccca
    37501 tgaccgtgat ccccagtaac gccgccagct gtgcgttatt gcccagctca ggatgtacgt
    37561 caagcggcaa atgccagcca tacaggttga tatcattcgc cagcaacgtt tttaaacggt
    37621 tacgcttcat tccgcgaatg accggagact ctcctttcca gaagtagcca tgatgcacaa
    37681 tgaccgcatc cgcgcccaga cgcactgcct catcgagcaa agcctggctg gcggtgacac
    37741 cggtaacaat tttttgcacc gtctctttgc cttctacctg caaaccattc ggcgcgtaat
    37801 cactaatcgc cgcgctgttc agtttttcgt taatcagttg ttccagttcg gtgtttttca
    37861 tcttcgcgcc ttccgtattc tgatagctac accgttaaca tagcgttacc tcgcctgttc
    37921 gtcgatagcc aatcttttga catattaaga aacaaacccg acgccaaaag acgcaaattg
    37981 tttatatcat cacgcttcag ctaatgtata tttatgcaat cagacagcat attgcataaa
    38041 taatcagatc tgtatcaaag ttttaacttt aaggaaacac aagcacgccg cgcaactatt
    38101 acgtgacgtt gcagtcaatt gtggaattat tatgaataaa cacgcatcac aaccgcgcac
    38161 tatttactat gtcgttgcgc tgcaaatctg ggaatatttt agcttttacg gcatgcgtgc
    38221 cctgctgatt ctctatctca ccaatcaact aaaatacaac gatactcacg cctacgagtt
    38281 atttagcgcc tactgttcgc tggtgtatgt cacgccaatc ctcggtggct ttttggcgga
    38341 taaagttctc ggcaatcgca tggcggtgat gctgggggcg ttgttgatgg cgatcggtca
    38401 tgtggtgctg ggtgccagtg agatccatcc gtcattcctc tatctgtccc tggcgattat
    38461 cgtttgtggc tatggtctgt ttaaatccaa tgtcagttgc ctgctcggcg agctgtatga
    38521 gccaaccgat ccgcgtcgtg atggcggttt ctcgctgatg tatgcagcgg gtaacgtggg
    38581 gtctattatc gcacctatcg cctgtggtta cgcccaggaa gagtacagct gggcgatggg
    38641 ctttggcctg gcggcggtgg gtatgatcgc gggtctggtc attttcttat gtggcaatcg
    38701 tcatttcaca catacccgcg gcgttaataa aaaagtactg cgtgcaacaa actttctcct
    38761 gcctaactgg ggatggctgc tggttctgct ggtggcaacg cctgcgctga ttaccgtact
    38821 gttctggaaa gagtggtcgg tatacgcctt aattgtcgcg actattattg gcctgggtgt
    38881 actggcaaaa atttatcgca aagcagaaaa ccagaaacag cgtaaggagc tggggctgat
    38941 tgtgacgctc accttcttca gtatgttgtt ctgggccttc gcacaacagg gcggcagctc
    39001 aattagcctt tatatcgacc gcttcgttaa ccgcgatatg tttggttata ccgttccgac
    39061 tgctatgttc cagtcgatta atgccttcgc agttatgcta tgcggtgtgt tcctggcgtg
    39121 ggtggttaaa gaaagcgtcg caggtaatcg taccgtgcgc atctggggaa aatttgctct
    39181 tggccttggc ctgatgagcg ccggattctg cattctgacc ttaagcgccc gctggtccgc
    39241 aatgtatggt cactcttctc tgccactgat ggtgttaggc ctcgcggtga tgggctttgc
    39301 ggaactgttt atcgacccgg ttgccatgtc gcaaattacg cgcattgaaa ttccgggcgt
    39361 gaccggcgta ttaaccggca tctacatgct gctttctggc gcgatcgcga actatctggc
    39421 gggcgtgatt gccgatcaga catcgcaggc ttcgtttgat gcttccgggg cgatcaacta
    39481 ctccatcaat gcatatattg aagtatttga tcaaattacc tggggcgcac tggcgtgtgt
    39541 aggactggta ctgatgattt ggctgtatca ggcgctgaaa ttcagaaacc gcgcgctggc
    39601 gctggagtca taataggcca gcaccgatgc ctgatgcgac gcttgacgcg tcttatca
//
LOCUS       DAJTZL010000014        39477 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.014, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000014 DAJTZL010000000
VERSION     DAJTZL010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39477
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.014"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(234..1574)
                     /gene="fadL"
                     /locus_tag="OO544_000661"
     CDS             complement(234..1574)
                     /gene="fadL"
                     /locus_tag="OO544_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HCR6766340.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAVYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            1946..2230
                     /locus_tag="OO544_000662"
     CDS             1946..2230
                     /locus_tag="OO544_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HCR6766341.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            2410..3720
                     /gene="fadI"
                     /locus_tag="OO544_000663"
     CDS             2410..3720
                     /gene="fadI"
                     /locus_tag="OO544_000663"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HCR6766342.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLCDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SELTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            3720..5864
                     /gene="fadJ"
                     /locus_tag="OO544_000664"
     CDS             3720..5864
                     /gene="fadJ"
                     /locus_tag="OO544_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HCR6766343.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPDEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHAARPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            6067..6552
                     /gene="sixA"
                     /locus_tag="OO544_000665"
     CDS             6067..6552
                     /gene="sixA"
                     /locus_tag="OO544_000665"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HCR6766344.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            7236..7799
                     /locus_tag="OO544_000666"
     CDS             7236..7799
                     /locus_tag="OO544_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR6766345.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTCAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     KAWLVGAADAPDLGNFEANTTFQITYL"
     gene            7881..10523
                     /gene="yfcU"
                     /locus_tag="OO544_000667"
     CDS             7881..10523
                     /gene="yfcU"
                     /locus_tag="OO544_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
                     /protein_id="HCR6766346.1"
                     /translation="MPDHSLFRLRILPWCIALAMSGSYSSVWAEDDIQFDSRFLELKG
                     DTKIDLKRFSSQGYVEPGKYNLQVQLNKQPLAEEYDIYWYAGEDDASKSYACLTPELV
                     AQFGLKEDVAKNLQWSHDAKCLKSGQLEGMEIKADLSQSALVISLPQAYLEYTYPDWD
                     PPSRWDDGISGIVADYSINAQTRHEENGGDDSNEISGNGTVGVNLGPWRMRADWQTNY
                     QHTRSNDDDEFSGDETQKKWEWSRYYAWRALPSLKAKLALGEDYLRSDIFDGFNYVGG
                     SVSTDDQMLPPNLRGYAPDISGVAHTTAKVTVSQMGRVIYETQVPAGPFRIQDLGDSV
                     SGTLHIRIEEQNGQVQEYDISTASMPYLTRPGQVRYKIMMGRPQEWGHHVEGEFFSGA
                     EASWGIANGWSLYGGALGDENYQSAALGVGRDLSTFGAVAFDVTHSHTKLDKDTAYGK
                     GSLDGNSFRVSYSKDFDQLNSRVTFAGYRFSEENFMTMSEYLDASDSGMVRTGNDKEM
                     YTATYNQNFRDAGVSVYLNYTRHTYWDREEQTNYNIMLSHYFNIGSIRNVSISMTGYR
                     YEYDNQADKGMYISLSMPWGDNSTVSYNGNYGSGTDSSQVGYFSRVDDATHYQLNVGT
                     SDKHTSVDGYYSHDGSLAQVDLSANYHEGQYTSAGLSLQGGATLTAHGGALHRTQNMG
                     GTRLLIDADGVADVPVEGNGAAVYTNMFGKAVVSDVNNYYRNQAYIDLNKLPENAEAT
                     QSVVQATLTEGAIGYRKFAVISGQKAMAVLRLQDGSHPPFGAEVKNDNEQTVGLVDDD
                     GNVYLAGVKPGEHMSVFWSGVAHCDINLPDPLPADLFNGLLLPCQHKGNVAPVVPDDI
                     KPVIQEQTQQVTPTNPPVSVSANQ"
     gene            10543..11294
                     /locus_tag="OO544_000668"
                     /pseudo
     CDS             10543..11294
                     /locus_tag="OO544_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
     gene            11269..11817
                     /locus_tag="OO544_000669"
     CDS             11269..11817
                     /locus_tag="OO544_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6766347.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINGVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            11814..12284
                     /locus_tag="OO544_000670"
     CDS             11814..12284
                     /locus_tag="OO544_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6766348.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTLSDFDNAAVSSNITGLG
                     IQLKQAGQSFTINTPLMVNETDLPVLTAVPVKKSGVVLPEADFEAWATLQVDYQ"
     gene            12281..12805
                     /locus_tag="OO544_000671"
     CDS             12281..12805
                     /locus_tag="OO544_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6766349.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALPGFL
                     AFDSSSSASGAGIGIETAAGTSVPINNTTGVTLPLNQGNNSLNFNTWLQAKSGRDVTS
                     GDFSATVTATFEYF"
     gene            12792..13664
                     /locus_tag="OO544_000672"
     CDS             12792..13664
                     /locus_tag="OO544_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2544 domain-containing protein"
                     /protein_id="HCR6766350.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVTYGKYDSFAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVTSYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNEL"
     gene            complement(13786..14337)
                     /gene="smrB"
                     /locus_tag="OO544_000673"
     CDS             complement(13786..14337)
                     /gene="smrB"
                     /locus_tag="OO544_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HCR6766351.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIPEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            14503..15435
                     /gene="prmB"
                     /locus_tag="OO544_000674"
     CDS             14503..15435
                     /gene="prmB"
                     /locus_tag="OO544_000674"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCR6766352.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLLAAREYFAIYKD"
     gene            15470..16555
                     /gene="aroC"
                     /locus_tag="OO544_000675"
     CDS             15470..16555
                     /gene="aroC"
                     /locus_tag="OO544_000675"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HCR6766353.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            16559..17383
                     /gene="mepA"
                     /locus_tag="OO544_000676"
     CDS             16559..17383
                     /gene="mepA"
                     /locus_tag="OO544_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HCR6766354.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPSGDGCGAELQSWFAPLKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            17383..18192
                     /locus_tag="OO544_000677"
     CDS             17383..18192
                     /locus_tag="OO544_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HCR6766355.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            18192..18740
                     /gene="epmC"
                     /locus_tag="OO544_000678"
     CDS             18192..18740
                     /gene="epmC"
                     /locus_tag="OO544_000678"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HCR6766356.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     ALDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            18774..19052
                     /locus_tag="OO544_000679"
     CDS             18774..19052
                     /locus_tag="OO544_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HCR6766357.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQQ"
     gene            complement(19173..21179)
                     /gene="mnmC"
                     /locus_tag="OO544_000680"
     CDS             complement(19173..21179)
                     /gene="mnmC"
                     /locus_tag="OO544_000680"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HCR6766358.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLNEGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVALYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLTALNPNRLWVRKLLKGKAVKAG"
     gene            21338..22558
                     /gene="fabB"
                     /locus_tag="OO544_000681"
     CDS             21338..22558
                     /gene="fabB"
                     /locus_tag="OO544_000681"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HCR6766359.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            22833..24011
                     /locus_tag="OO544_000682"
     CDS             22833..24011
                     /locus_tag="OO544_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6766360.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     ELGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGFI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTEFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(24008..25003)
                     /gene="flk"
                     /locus_tag="OO544_000683"
     CDS             complement(24008..25003)
                     /gene="flk"
                     /locus_tag="OO544_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HCR6766361.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVNDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            25102..26238
                     /gene="pdxB"
                     /locus_tag="OO544_000684"
     CDS             25102..26238
                     /gene="pdxB"
                     /locus_tag="OO544_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HCR6766362.1"
                     /translation="MKILVDENMPYARDLFSRLGEVTAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQHADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            26304..27317
                     /locus_tag="OO544_000685"
     CDS             26304..27317
                     /locus_tag="OO544_000685"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCR6766363.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            27317..28129
                     /gene="truA"
                     /locus_tag="OO544_000686"
     CDS             27317..28129
                     /gene="truA"
                     /locus_tag="OO544_000686"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HCR6766364.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPERWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            28212..28871
                     /locus_tag="OO544_000687"
     CDS             28212..28871
                     /locus_tag="OO544_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR6766365.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGDKLFS
                     NPDSKIFRRSYLDKTHQFYERHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLIFTYAGYFFGTLPFIQSNLKLMIVGIIFVSILPGVFEFIRHKRAAARAA
                     K"
     gene            29027..29941
                     /gene="accD"
                     /locus_tag="OO544_000688"
     CDS             29027..29941
                     /gene="accD"
                     /locus_tag="OO544_000688"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HCR6766366.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVHRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            30011..31279
                     /gene="folC"
                     /locus_tag="OO544_000689"
     CDS             30011..31279
                     /gene="folC"
                     /locus_tag="OO544_000689"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HCR6766367.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSAKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            31269..31931
                     /gene="dedD"
                     /locus_tag="OO544_000690"
     CDS             31269..31931
                     /gene="dedD"
                     /locus_tag="OO544_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HCR6766368.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            32190..32678
                     /gene="cvpA"
                     /locus_tag="OO544_000691"
     CDS             32190..32678
                     /gene="cvpA"
                     /locus_tag="OO544_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HCR6766369.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            32715..34232
                     /gene="purF"
                     /locus_tag="OO544_000692"
     CDS             32715..34232
                     /gene="purF"
                     /locus_tag="OO544_000692"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HCR6766370.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            34327..34896
                     /gene="ubiX"
                     /locus_tag="OO544_000693"
     CDS             34327..34896
                     /gene="ubiX"
                     /locus_tag="OO544_000693"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HCR6766371.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            35162..35944
                     /gene="argT"
                     /locus_tag="OO544_000694"
     CDS             35162..35944
                     /gene="argT"
                     /locus_tag="OO544_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HCR6766372.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKAFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            36165..36947
                     /gene="hisJ"
                     /locus_tag="OO544_000695"
     CDS             36165..36947
                     /gene="hisJ"
                     /locus_tag="OO544_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HCR6766373.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            37037..37723
                     /gene="hisQ"
                     /locus_tag="OO544_000696"
     CDS             37037..37723
                     /gene="hisQ"
                     /locus_tag="OO544_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HCR6766374.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            37720..38436
                     /locus_tag="OO544_000697"
     CDS             37720..38436
                     /locus_tag="OO544_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6766375.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            38444..39217
                     /gene="hisP"
                     /locus_tag="OO544_000698"
     CDS             38444..39217
                     /gene="hisP"
                     /locus_tag="OO544_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HCR6766376.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
BASE COUNT         9349 a         9672 c        10612 g         9844 t
ORIGIN      
        1 gtatcctgga aatgcagaaa agtaaactct ttgagaagga cgagcagaac gcctgaaaat
       61 atgcctttcc cttcaggttt gtctgtggat accgcttatt gatttgaaag ggcaagctgc
      121 caacaggctg tttgcaaaac tcaggaggta agcaatgagg acactacttt cggtgaagtg
      181 ggggagttaa agtcacctgc tatgcaggtg actttatcca ggcgaacgcg ttatcagaac
      241 gcgtagttaa agttagtacc gaacagccag gctttacctt cagactcgaa ctggtatggg
      301 ccttcgttaa ttttcacgct ctgaccgtgc atataagaaa caccaacgtc gactgaagca
      361 tctttattaa atgcgtaagt cgtacctgca ctcagccaga aacggtcctg gtccggaatg
      421 gagatagaac gattctgagc cggaactggg ctgtcatcaa aggcgatacc ggtacggaag
      481 gtccagttat catcgtagta ataagtggta cccaacgcga tgcggtaagc atctttaaag
      541 ccttcatgtt tctggaacag cgtgtcgcca ctggttgagg tcgctttcag ctgctggaac
      601 tgactccagc tggtgtaagc caggctatag tgaatcgccc actgtggatc aacacggtta
      661 taacctgaca cttcccacat ttcaggcagg ttcagcgtca gataacccga ttgcgttgcg
      721 ccacctgtcg cggtaggaat tggcaaaccg tagttattaa acgcacgatt aagatcgctg
      781 ctgtagttac ctttgaagtc aattttcact tcagaacggt aggtcagtgc atagcggtta
      841 tttttatcca gttcatacag gattccggcg ttccagccaa agccccactg gttaccgttc
      901 agatgagcga ttttggtatt actgtcgata ccgttggcgg tagctgccaa tgcttgccct
      961 tgaggagttt gcccggcagg agattgcata atctgaccag caaccaactg ccccagatcg
     1021 cctgcgaaac gttcaatttt cgcgcgagcg tagacggcgt tgaaaccaag accaaagctc
     1081 catgcattat ttaagcgata cgcaccgctt aagttcaggt tcatggtttc aaggtcagtt
     1141 gtacccccga cagagccgcc tgcataagta tcattaaact ctgttgccag accatagtta
     1201 gaggtaatag aagcgcccca accaaattgg tcgttaatcg gtgcaacaaa gtgcatgttc
     1261 ggaacccatg ccgtaggcgc gatgttatcg gctttcaggc tacgaccaga cggagacgtt
     1321 ccgctgatat tcacatccgg gtcaatataa accgcacccg cagaaaatgt cgggcggtca
     1381 aacatggtaa tcaatgcggg gttacggcta acgttacctg catcatcggc aattgcgcct
     1441 tcccctgaat aagcccggcc caggccagag gaagaaaatt cgtttaactg aaagcctgcc
     1501 gaccaggcct gggtggagat aagtgccact gcgactgcga gagcagactt tgtaaacagg
     1561 gttttctggc tcatgaccat aacctcaatg atttattttt atacaaacta tgttacgtgc
     1621 tgtaacagga gcgcgaagtg tagggtctga ggtacatctt agaaatcaga ccagtggcga
     1681 gagtataggt cggaccagct ggaatgctgc aagtatgttt ccatcttttt tcaaatatga
     1741 tctaagaaac gcgatccagc tggcaaaatt tggaggttgc gcactcgcgg attcgggtga
     1801 attttgtttt agatcatttt taagtgtgat ttcggtcact tatccgattt tgcagaatta
     1861 acaactggag cagcactttc cggaagcgta aaatatccac aacgtttatc tggcaccttg
     1921 ggtgcacata cagaggaaat caacgatgag taaatgtagt gctgatgaaa ccccggtttg
     1981 ctgctgtatg gatgttggca ccattatgga caactccgac tgcaccgcgt cttacagccg
     2041 cgtgtttgca aaccgcgcag aagcagaaca aacgctggcg gcgctgactg aaaaagcccg
     2101 tagcgtggaa tccgaaccgt gcaaaatctc cccgactttc actgaagagt ctgacggtgt
     2161 gcgcctggat atcgatttca cttttgcctg cgaagctgaa atgctgatct tccagctggg
     2221 tctgcgttaa ttcctctcgg gtacgttcgc ccctgtcagt tgctggcagg ggcgttttct
     2281 tttctttctc ctgtcttcac tgtgtcatag ctcccacttt cctttttctc cgcttggcta
     2341 aatgtaaaaa aatagttaag actatgatca ggtcagacca ctttatttat ttttttacag
     2401 gggagtgtta tgggtcaggt tttaccgctg gttacccgcc agggcgatcg tatcgccatt
     2461 gttagcggtt tacgtacgcc ttttgcccgc caggcgacgg cttttcatgg cattcccgcg
     2521 gttgatttag ggaagatggt ggtaggcgaa ctgctggcac gcagcgagat ccccgctgaa
     2581 gtgattgaac agctggtctt tggtcaggtc gtacaaatgc ctgaagcccc caacattgcg
     2641 cgtgaaattg ttctcggtac cggaatgaat gtgcataccg atgcttacag cgtcagccgc
     2701 gcttgcgcta ccagtttcca ggcggttgca aacgtcgcag aaagcctgat ggcgggaact
     2761 atccgagcgg ggattgccgg tggggcagat tcctcttccg tattgccaat tggcgtcagt
     2821 aaaaaactgg cgcgcgtgct ggttgatgtc aacaaagctc gaaccatgag ccagcgcctg
     2881 aaactcttct ctcgcctgcg tttgtgcgac ttaatgcccg taccgcctgc ggtagcagaa
     2941 tattctactg gcttgcggat gggcgacacc gccgaacaaa tggcgaaaac ctacggcatc
     3001 acccgagaac agcaagatgc attagcgcac cgttcgcatc agcgtgccgc tcaggcatgg
     3061 tcagacggaa aactcaaaga agaggtgatg actgccttta tccctcctta taaacaaccg
     3121 cttgtcgaag acaacaatat tcgcggtaat tcctcgcttg ccgattacgc aaagctgcgc
     3181 ccggcgtttg atcgcaaaca cggaacggta acggcggcaa acagtacgcc gctgaccgat
     3241 ggcgcggcag cggtgatcct gatgactgaa tcccgggcga aagaattagg gctggtgccg
     3301 ctggggtatc tgcgcagcta cgcatttact gcgattgatg tctggcagga catgttgctc
     3361 ggtccagcct ggtcaacacc gctggcgctg gaacgtgccg gtttgacgat gagcgaactg
     3421 acattgatcg atatgcacga agcctttgca gctcagacac tggcgaatat tcagttgctg
     3481 ggtagtgaac gttttgctcg tgaagtactg gggcgtgcac atgccactgg cgaagtggac
     3541 gatagcaaat ttaacgtgct tggcggttcg attgcttacg ggcatccctt cgcggcgacc
     3601 ggcgcgcgga tgattaccca gacattgcat gaacttcgcc gtcgcggcgg tggatttggt
     3661 ttagttaccg cctgtgctgc cggtgggctt ggcgcggcaa tggttctgga ggcggaataa
     3721 tggaaatggc atcagcgttt actcttaatg ttcgtctgga caacattgct atcatcacca
     3781 tcgatgtacc ggatgagaaa atgaataccc tgaaggcgga atttgcctcg caggtgcgcg
     3841 ccattattaa gcaactccgt gaaaacaaag agttgcgcgg cgtggtgttt atctccgcga
     3901 aaccggacaa ttttatcgcc ggagcagaca tcaacatgat tggcaactgc aaaacggcgc
     3961 aagaagcgga agcactggcg cggcagggcc aacagttgat ggcggagatt catgctttgc
     4021 ccattccggt tatcgcggct attcatggcg cttgcctggg cggtgggctg gagctggcgc
     4081 tggcgtgcca cggtcgcgtt tgtactgacg atcctaaaac ggtgctcggt ttgcctgaag
     4141 tacaacttgg attgttaccc ggttcaggcg gcacccagcg tttaccgcgt ctgataggcg
     4201 tcagtacagc attagagatg attctcaccg gaaaacaact tcgggcgaaa caggcattaa
     4261 agctggggct ggtggatgac gttgttccgc actccatttt gctggaagcc gctgttgagc
     4321 tggcgaagaa ggatcgccca tcttcccgcc ctctacctgt acgcgagcgt attctggcgg
     4381 ggccgttagg tcgtgcgctg ctgttcaaaa tggtcggcaa gaaaacagaa cacaaaactc
     4441 aaggcaatta tccggcgaca gaacgcatcc tggaggttgt tgaaacggga ttagcgcagg
     4501 gcaccagcag cggctatgac gctgaagctc gcgcctttgg cgaactggcg atgacgccgc
     4561 aatcgcaagc tttgcgtagt atcttttttg ccagtacgga cgtgaagaaa gatcccggta
     4621 gtgatgcacc gcctgcgcca ttaaacagcg tgggaatttt aggtggtggc ttgatgggcg
     4681 gcggtattgc ttatgtcact gcttgtaaag cggggcttcc ggtcagaatt aaagatatca
     4741 acccgcaggg cataaatcat gcgctgaagt acagttggga tcagctggag ggcaaagttc
     4801 gccgtcgtca tctcaaagcc agcgaacgtg acaaacagct ggcattaatc tccggaacga
     4861 cggactatcg cggctttgcc catcgcgatc tgattattga agcggtgttt gaaaatctcg
     4921 aattaaaaca acagatggtg gcggaagtgg agcaaaattg cgccgctcat accatttttg
     4981 cttcgaacac gtcatcttta ccgattggtg atatcgccgc tcacgccgcg cgacctgagc
     5041 aagttattgg cctgcatttc ttcagtcctg tggaaaaaat gccgctggta gagattattc
     5101 ctcatgcggg tacatcggcg caaaccatcg ccactacggt aaaactggcg aaaaaacagg
     5161 gcaaaacgcc cattgtcgtg cgtgacaaag ccgggtttta cgtcaatcgc atcttagcgc
     5221 cttacattaa tgaagctatc cgcatgctga ccgaaggtga acgggtagag cacattgatg
     5281 ccgcgctagt gaaatttggt tttccggtag gcccaatcca acttttggat gaggtaggaa
     5341 tcgacaccgg gactaaaatt attcctgtac tggaagccgc ttatggagaa cgttttagcg
     5401 cgcctgcaaa tgttgtttct tcaattttga acgacgatcg caaaggcaga aaaaatggtc
     5461 ggggtttcta tctttatggt cagaaagggc gtaaaagcaa aaaacaggtc gatcccgcca
     5521 tttacccgct cattggcgca caagggcagg ggcgactctc cgcaccgcag gttgctgaac
     5581 ggtgtgtgat gttgatgctg aatgaagcag tacgttgtgt tgatgagcag gttatccgta
     5641 gcgtgcgtga cggggatatt ggcgcggtat ttggcattgg ttttccgcca tttctcggtg
     5701 gaccgttccg ctatatcgat tctctcggcg cgggcgaagt ggttgcaata atgcaacgac
     5761 tggccacgca gtatggttcc cgttttaccc cttgcgagcg tttggtcgag atgggcgcgc
     5821 gtggggaaag tttttggaaa acaactgcaa ctgaccttca ataagaaggt caaagctata
     5881 tgaatccgcg ctgaatggcg gagtgttggt caaaatgtaa acgcatattg actatactta
     5941 cgccattgag gtaaaaaaca gcgtttcatt cggtgaatgg ataaggcaca atgccggcca
     6001 ccgttttctt tctctggttt cagatgaaag aaaacggacg aatctggtta acaaaagcgg
     6061 tgcaatatgc aagtttttat catgcgtcac ggcgacgcag cccttgatgc cgccagtgat
     6121 tcggttcgtc ctctgaccac taatggttgt gacgaatctc gcctgatggc gaactggctg
     6181 aaaggtcaaa aagtggaaat cgaacgcgtt ctggtgagcc cgttcctgcg agccgagcaa
     6241 acactggaag aagtagggga ttgtctgaac ctgccttcca gtgcagaagt tctgccggag
     6301 ttaacgccct gcggcgatgt tggtctggtc agtgcctatt tgcaggcgtt gaccaatgaa
     6361 ggtgttgctt cggtactggt gatctctcac ttaccgttag tcgggtatct ggttgccgag
     6421 ttatgtccgg gcgaaacgcc gcctatgttt accacctcgg ccattgccag cgtaacgctt
     6481 gatgaaagcg gaaacggcac attcaactgg cagatgagcc cgtgtaatct gaagatggca
     6541 aaagctatct gatgagttcc aaattatgcc caatgaggta gattcattgg gcataattgc
     6601 tcatcaggca gcggatatct gtcaatctca ctactatcga agactctccc catctataaa
     6661 taaaacactt tctactttca ttcggaaatt attcttctgc gcgtatcttg attttgccca
     6721 aagttacatg ttgaaatgaa taatgtctgt ttgacactca ttgtcattta ttaatacaga
     6781 taatagagaa aatagagttg aataatcagg caggatgtaa ttgaaacttg attaaattct
     6841 ggctatagtg tttatatatt atattgtcaa aagtggataa atgccaatcg cattatatgt
     6901 tcattgtttg ttttttaagc tgttgatatt cattattgat atactttcta ttttttctaa
     6961 atacttattc cgtatttttt aaggatgtcc ttaagataac ctttcttctg tcaatatatt
     7021 aattaaatct acatttactc tttagtaaaa ttaaattgca tcaggggtgt ggaaacatca
     7081 ctatgcggcc ccacaccaac gaatacaatt ttctacgacc tgttcttttt ggatcgcatg
     7141 ccgtattcaa tggctgggta taaacattgg ttattgactg aatcaatgta ttgcaatgtt
     7201 tatattttta attatttata gtttaaggac aaataatgag taagtttgtt aaaacagcta
     7261 ttgctgcggc aatggtgatg ggcgtgttca cctctacggc aacaatcgct gcgggtaaca
     7321 acggtactgc acgtttttac ggcaccattg aagactcagt gtgttctatc gttccggacg
     7381 atcacaaact ggaagttgat atgggtgata tcggtgccga aaaactgaaa aataacggca
     7441 ccaccacgcc gaaaagtttc cagattcgtc tgcaagattg cgtatttgat actcaggaaa
     7501 caatgaccac tacctttact ggtaccgttt cttctgcaaa tagcggcaat tattacacca
     7561 ttttcaatac cgatacctgt gcggcattta acaatgttag cctggcgatc ggtgactctc
     7621 tgggtacctc ttacaaaagc ggcatgggta ttgaccagaa aatagtgaaa gacacttcta
     7681 ccaataaagg caaagcgaag cagacactga actttaaagc ctggctggtc ggcgcagctg
     7741 atgcgccaga tctgggtaat ttcgaagcaa ataccacctt ccagattact tacctgtaag
     7801 gcataccccc atagcgacac tgctatgggg gatttaaatg gattttgacc gaacaagaat
     7861 aaaaacggat agaaacgtgt atgcctgacc attctctttt tcgattacgg attcttccgt
     7921 ggtgcattgc gctggcaatg tcagggagtt atagcagtgt ctgggctgaa gacgacattc
     7981 agtttgattc ccgttttctg gaattaaaag gcgacacaaa aattgatctg aagcgttttt
     8041 ccagccaggg atatgttgag cccggaaaat acaatttaca ggttcaacta aataaacagc
     8101 cattggcgga agagtacgat atttactggt atgctggtga agatgacgcg agcaaaagct
     8161 atgcttgtct gacaccggaa ctggtagcgc agtttggttt aaaagaagac gtggcgaaaa
     8221 atctgcaatg gagccacgat gctaaatgcc tgaaatccgg tcaactggaa ggcatggaaa
     8281 ttaaggctga tttaagccag tccgcattag tcatttcact gccacaggct tacctcgaat
     8341 atacttatcc cgactgggat ccgccttcac gttgggatga cggcatctcc gggatcgtcg
     8401 cggactacag catcaacgca caaacccggc acgaagaaaa tggcggtgat gatagtaacg
     8461 agatcagcgg caacgggacg gtcggggtta acctggggcc gtggcgtatg cgtgctgact
     8521 ggcagactaa ctatcaacat actcgcagta atgatgacga tgaattcagc ggcgatgaaa
     8581 ctcaaaaaaa atgggagtgg agtcgctact atgcctggcg ggcgttacca tcattaaaag
     8641 ccaaactggc gctgggcgag gattacctca gatccgatat ttttgatggt tttaactatg
     8701 ttggtggcag tgtcagtact gacgatcaaa tgttgcctcc caatctgcgc ggctacgcgc
     8761 cagacatttc cggcgtggca cacaccacag caaaagtgac cgtcagccag atggggcgtg
     8821 tgatttacga aacgcaggtt ccggctggac cgtttcgtat tcaggatctt ggtgattctg
     8881 tctccggtac gttgcatatt cgcattgaag aacagaacgg ccaggtgcag gaatatgaca
     8941 tcagcaccgc ctcgatgcca tacctcactc gtccaggtca ggttcgctat aagatcatga
     9001 tgggccgtcc gcaagagtgg ggacaccatg tcgagggtga atttttttct ggtgctgaag
     9061 cttcctgggg gatcgctaac ggctggtcgt tatatggcgg cgcactggga gatgaaaact
     9121 atcagtctgc ggcgcttggc gtcggtcgcg atttgtctac attcggcgcg gtcgcgtttg
     9181 atgttactca ctcgcacacc aaactggata aagacaccgc ttatggcaaa ggttcgctgg
     9241 acggtaactc cttccgtgtg agttattcca aagactttga ccagctcaac agccgcgtta
     9301 cttttgctgg atatcgcttc tcggaagaga actttatgac catgagcgag tatctggatg
     9361 ccagtgacag cggaatggta cgcacgggca acgacaaaga gatgtacact gccacttata
     9421 accagaactt ccgcgatgcg ggtgtttcgg tttatctcaa ctatacccgc catacctact
     9481 gggatcgcga ggagcagaca aactacaaca tcatgctctc gcactatttc aatataggca
     9541 gtattcgtaa tgtcagcatc tcgatgactg gctaccgtta cgagtatgac aaccaggccg
     9601 acaaaggcat gtacatttcg ctcagtatgc cgtggggtga caacagtacc gttagctata
     9661 acggtaacta tggcagtggg acggacagca gtcaggtcgg ttatttcagc cgtgtcgatg
     9721 acgcgactca ctatcagttg aacgtcggca ccagtgacaa acacaccagc gttgacggct
     9781 attacagcca tgatggttcg ctggcgcagg ttgacctcag tgcgaactac catgaagggc
     9841 aatacacctc tgcgggcttg tcgttacagg gcggcgcaac gcttactgcc cacggtggcg
     9901 cacttcaccg tacccagaat atgggcggga cacgcttgtt gattgatgcc gatggcgttg
     9961 ccgatgttcc ggtagaaggt aacggtgctg ctgtttatac caatatgttt ggtaaagccg
    10021 tcgtttctga cgtcaataac tattaccgca atcaggcgta tatcgacctc aacaaactgc
    10081 cggaaaacgc cgaagcaacc cagtcggtgg tacaagccac gctaactgaa ggggcgattg
    10141 gctaccgcaa atttgccgtc atcagtggtc aaaaagcgat ggcggtgctg cgtttacaag
    10201 acggcagcca tcctccgttt ggcgcagaag taaaaaatga taacgagcag acagtgggcc
    10261 ttgtcgatga tgacggcaat gtttatctgg ctggggtgaa acctggcgaa cacatgagtg
    10321 tgttctggag tggtgttgcg cattgcgata tcaacctgcc ggacccgctg cctgccgatc
    10381 tgtttaacgg cttgttactg ccatgccagc ataaaggcaa cgtcgcgccg gttgttcctg
    10441 atgacataaa gcctgtcatt caggagcaga cgcaacaggt gacacccacc aatccccctg
    10501 tttctgtttc agctaaccaa taacgtgatt aaggaatgat ccatgtctga tttactttgc
    10561 tctgccaaat tgggtgctac gacgctagcg ttactgctta gcgctgccag tttgtcgccc
    10621 aggcttctgt aacgccagat cgtacccgtt tgatatttaa tgaaagcgac aaatcaatca
    10681 gcgtgacgtt gcgcaataac gatccgaaac tgccttatct ggcccaaagc tggatcgaag
    10741 atgaaaaggg taacaagatc agttcaccgt taactgttct gccaccggtg caacgcatcg
    10801 attcaatgat gaacgggcag gtaaaagtgc agggaatgcc ggatatcaac aaattgcctg
    10861 ccgatcgtga aagcctgttc tacttcaacg tgcgtgagat cccgccaaaa tcaaacaaag
    10921 ccaacacgct gcaaatagca ctacaaacgc gtattaagtt gttctggcga ccgaaggcgc
    10981 tggagaacgt cagcatgaaa aacccctggc aatataaggt tactttaacc cgcaacggtc
    11041 aggaatttac cgtcaataac ccgacgccgt attacgtcat tttcagtaat gccagcacac
    11101 agaaaaatgg caatcctgca gccggtttct cgccaatagt gatgtcgccg aaaacctctg
    11161 caccgctgaa tgtgaagatg ggtagcgtgc cggtactgac ctatgtcaac gactatggcg
    11221 ctcggatgcc gctctttttc acttgcaacg gtaacagttg ccaggtcgat gaagagcaga
    11281 gccgcaaagg ctgaggccgg gagagccagt atgaaaggat cttgttctgt ctgggcggta
    11341 actgggttac tgatatgttg tgttggtagc ggctttgcag ttgcagcgag taagtcagta
    11401 agtatgaatt tacgggtgct tgtcgatgcc cctcctcctt gtacggtaaa tggtgcggca
    11461 gttgagtttg gtaatgtatt cattaataag atcaatgggg ttgattataa acgtcccatt
    11521 gactactcgc ttgtttgtaa caatctggca atggatgacc tgcgtctaca gatgcaggcg
    11581 acaacggtcg tgattaacgg tgaaacagtt atcagcacgg gtattcctgg gtttggtatt
    11641 cgggttcaga aaagtagtga tcacacaata ctcgacttaa cctctggctc atggttgcca
    11701 tttaattttt cctcgggagt tcccgttctg gaagctgtgc cggtgaaaca gagtggtaca
    11761 acgttagcgg cagctgaatt taacgccagt gccacaattg tggtggatta ccaatgaaac
    11821 ggatctcgct tattttgctg tggggctttt gctcgatggc actctcaaac gtctcttttc
    11881 acggttattt agtgcagccg cctaactgca cgattagcaa cgctcaaacc attgaaataa
    11941 cttttcagga tgtgcttatt gatgacatta atggcagcaa ttatgagcaa accgtgccgt
    12001 attccattac ctgcgatacg gcggttcgcg acccattaat ggaaatgacc ttgagctgga
    12061 gtggcacact aagcgatttt gataatgctg cagtaagcag caatatcact ggtttaggca
    12121 ttcagctcaa acaggcaggg cagagtttta cgatcaatac gccacttatg gtgaatgaaa
    12181 ccgatttacc ggtgttaacg gctgtgccag taaaaaagag tggtgtcgtt ctgccagagg
    12241 cggactttga ggcctgggcc acgttacagg tagattacca atgaagaaaa agcgtacgtt
    12301 gttttttatt agtagcctga tgttgctggg cagtggaacc acaatagcag gagataattt
    12361 gcactttacc ggcaatttaa tcagtaaatc gtgtacgcca gtgattaatg gtagccagtt
    12421 ggcagaagta cattttcctg ccattgccgc cagcgattta atgaaccttg ggcagtcaga
    12481 gcgagtgcca ttggtgtttc aattgaaaga ttgtcatagt tctacattgt ttaatgtcaa
    12541 agtaacgttg acaggaacag aagatagtgc tctacccggc tttctggcct ttgactcctc
    12601 gtcatcagca tccggcgctg ggattggtat tgaaactgcg gcaggcacat cggtgccaat
    12661 caacaataca acaggcgtca cgctcccgct gaatcaggga aataacagcc taaattttaa
    12721 tacctggtta caggcaaaga gcgggcgaga tgtaacgtcc ggagacttta gtgccacagt
    12781 gacggcaacc tttgagtatt tctgaaatga aaaaatggac cataattttc gcgagcctaa
    12841 tgctgctcgt tctgagtgtt gtcggcgcat ctaagagtta tgctggagat aaacttatga
    12901 gtgcgagttt tgacagtgtg agagtttatt atgccatgga caaggtaacc ggtgctgtag
    12961 cgagcagtgt tttcaatgtt acggtgataa cgccaaagga ggttacatat gggaaatatg
    13021 attcttttgc gtataaaggc gatacgttgc gggttatttc ctggtctggg tcgggttcag
    13081 ctccaacttt ggtactgact gactttgata caattaataa ctcaaattgc cctggcatag
    13141 atacgaaaat ttttcgttgt gcatatatga cgtttaaaat taccgttgca tctgatgatt
    13201 atggttgccc ttggatcgca tccttttatt cctatacaga tttacccgga tttggaagct
    13261 atacggcccc tacagtgcat aacacaatat gcccaacgat accggtcact tcatacgata
    13321 tctcctggag tgaaaattat gtcagccata ataaggcgtt acgaattcag tccacggggt
    13381 caacagtgac caccacgctt tcgacctacc tgatggaagg cggcagactc tgtgatggca
    13441 gtaatttttc tgataatgat ggcagaggag catactgccg cgcggtatca gaattgttaa
    13501 cctttacctc ttacggttgt gataaatcaa cggtaacggt tacgccaact cgtcatccgg
    13561 taacggataa agtactacac gatattgtgg tgaatgtgaa taccagtagt gggcaaccga
    13621 ttgattcgac gtgtcgtttt cagtatgtat tgaatgaact gtagttcgta gtattgctct
    13681 gatgcgtctt atcaggccta cgaagtgaat tgcaatttat tgaattatca catatttgta
    13741 ggccggataa ggcgtttacg ctgctccggc atttgctgcc agcattcagg gcaactccgg
    13801 cggcaaccac tcttccactt caatcaacac caacaaagcc gcatcaccgc cgtactcttt
    13861 cggtgcctgg tgaaatgcca tcacatgcgg atgttgtgcc agccacagcg gtgtttgttg
    13921 cttcaaaata tgcttcccgt gcccatgcat cacgcaggca caaaacacat gttcgcggcg
    13981 acaggcggca atcaacgcgc ccagttcctg cttggcctgc agctgcgtca gaccgtgtaa
    14041 atccaaaaac aattccggtg aataatcgcc gcggcgcagt ttcttcgcct caaaatggct
    14101 gacatccggg cgaacatatt tcaccggacc ttcggtattt aataacggct gaaactcatc
    14161 ggagaaataa tggctggcat cagcctgctc cgggatcaag cgtttcaccg gcacttcgct
    14221 gattttttta cgctgcggtc ggtggacaat cgtgtcctgc ttaatcttgc gagtccccgc
    14281 catcaactgg cgaaacagag cctggtcctc ctcgctaagt gttgttttct ttttcattta
    14341 tctgtctcta tttctaattt tcctacagtt tacccgactc gtagcacaat cgatcgtttt
    14401 aaacgtattt ttcctcactc accgcgcgtg aatgctgatt tatcgtcatc ttcatggcaa
    14461 actagccgcc gaaattattg cgatcatgcc ctggaggaat acgtggataa aattttcgtt
    14521 gatgaagcag taaatgagct gcaaaccatt caggacatgt tgcgctggtc ggtaagccgc
    14581 ttcagcgcgg caaatatctg gtacggtcac ggcaccgata acccgtggga tgaagccgta
    14641 cagctggtgt taccatcgct ctacctgccg ctggatatcc cggaagatat gcgcaccgcg
    14701 cgtctgacct ccagcgaaaa acaccgtatt gttgaacgcg tgatccgccg cgtcaacgag
    14761 cgcattccgg tggcttacct gaccaacaaa gcctggttct gcggtcatga attttacgtc
    14821 gatgaacgcg tgctggtgcc gcgctcgccg attggtgaac tgatcaacaa taaatttgcc
    14881 ggactcatta gcaagcaacc gcagcatatt ttagatatgt gtaccggtag cggctgcatc
    14941 gccattgcct gtgcttatgc cttcccggaa gcggaagtcg acgcggtaga tatctctccc
    15001 gacgcgctgg cggttgccga acagaacatc gaagaacacg gtctgatcca caacgtcatt
    15061 ccgattcgtt ccgatctgtt ccgtgatttg ccgaaagtgc agtacgatct gattgtcact
    15121 aacccgccgt atgtcgacgc ggaagatatg tccgacctgc caaacgaata ccgtcacgag
    15181 ccggaactgg gcctggcatc tggcaccgac ggccttaaac tgacgcgtcg cattcttgga
    15241 aacgcggcag attaccttgc tgatgatggc gtgttgattt gtgaagtcgg caacagcatg
    15301 gtacatctta tggaacaata tccggatgtt ccgttcacct ggctggagtt tgataacggc
    15361 ggcgatggtg tgtttatgct caccaaagag cagctgctgg cggcccgtga atatttcgcg
    15421 atttataaag attaagtaaa cacgcaaaca caacaataac ggagccgtga tggctggaaa
    15481 cacaattgga caactctttc gcgtaaccac cttcggcgaa tcgcatgggc tggcgctcgg
    15541 ctgcatcgtc gatggtgttc cgccaggcat tccgctgacg gaagcggacc tgcaacatga
    15601 cctcgaccgt cgtcgccctg ggacatcgcg ctataccacc cagcgccgcg agccggatca
    15661 ggtcaaaatt ctctccggtg tttttgaagg cgttactact ggcaccagca ttggcttgtt
    15721 gatcgaaaac actgaccagc gctctcagga ttacagcgca attaaagacg ttttccgccc
    15781 aggccatgct gattacacct acgaacaaaa atacggtctg cgcgattatc gcggcggtgg
    15841 acgttcttcc gcccgcgaaa ccgccatgcg cgtggcggca ggggcgattg ccaaaaaata
    15901 tctcgccgag aaatttggta ttgaaatccg cggctgcttg acccagatgg gcgacattcc
    15961 gctggaaatc aaagactggt cgctggttga acaaaatccg ttcttctgcc cggacccgga
    16021 caaaatcgac gcgttagatg aactgatgcg cgcgctgaaa aaagagggcg actccatcgg
    16081 cgcgaaagtc accgttgttg ccagtggtgt ccccgccgga cttggcgagc cggtctttga
    16141 ccgcctggat gccgacatcg cccatgcgct gatgagcatc aacgcggtga aaggcgtgga
    16201 aattggcgac ggttttgacg tagtggcgct gcgcggcagc cagaaccgcg acgaaatcac
    16261 caaagacggt ttccagagca accatgcggg cggcattctc ggcggtatca gcagcgggca
    16321 gcaaatcatt gctcatatgg cgctgaaacc gacctccagc attaccgtgc cggggcgcac
    16381 tattaaccgc tttggcgaag aagttgagat gatcaccaaa ggccgtcacg atccctgtgt
    16441 cgggatccgt gcagtgccga tcgctgaagc gatgctggcg atcgttttaa tggatcacct
    16501 gttacggcag cgggcgcaaa atgccgatgt gaagactgat attccacgct ggtaaaaaat
    16561 gaacaaaacc gcgattgcgc tgctggctct gcttgccagt agcgctagcc tggcagcgac
    16621 gccgtggcaa aaaataaccc aacctgtgcc gggtagcgca caatcgatag gcagtttttc
    16681 taatggctgt attgtcggcg ctgacacgtt gccgatacag tccgaacatt atcaggtcat
    16741 gcgtaccgat cagcgtcgct atttcggtca cccggatctg gtgatgttta tccagcgtct
    16801 gagtagccag gtgagcaatc tgggcatggg tacggtgctg attggcgata tggggatgcc
    16861 cgctggtggg cgtttcaacg gcggtcatgc cagccaccag accggactgg atgtcgatat
    16921 ctttctgcaa ctgccgaaaa ctcgctggac ctccgcgcag ctcttgcgcc cgcaagcact
    16981 ggacttagtt tcccgcgacg gtaaacacgt tgtctccacg ctgtggaagc cagaaatttt
    17041 cagcttgatc aaactcgctg cccaggacaa agacgtcacg cgcatttttg ttaatccggc
    17101 gattaaacaa caactttgcc ttgatgcggg caccgatcgc gactggttgc gcaaagtgcg
    17161 accctggttc cagcatcgcg cgcatatgca tgtacgatta cgttgccctg ccgatagtct
    17221 ggagtgtgaa gatcaacctt taccgccatc aggcgatggt tgcggggcag aactgcaaag
    17281 ctggtttgca cctctaaaac cgggaacaac aaagcctgag aagaagacac cgcctccgtt
    17341 gccgccttcc tgccaggcgc tactggatga gcacgtgatc taatggaaac gtttaatagc
    17401 ctgtttatgg tttccccgct gttgctggga gttctctttt ttgtcgccat gctggcggga
    17461 tttatcgact cgattgccgg tggcggtggg ttactcacca ttccggcatt gatggctgcg
    17521 ggaatgtctc ccgctaacgc gctggcaacc aataaactgc aagcctgcgg cggctctatt
    17581 tccgccacta tctactttat tcgccgcaaa gtggttagtt taagcgatca gaaactcaat
    17641 attgccatga cctttgtcgg ctcaatgagc ggcgcactgt tggtacagta cgttcaggct
    17701 gatgtcttac ggcagatttt gcccattctg gtgatttgta tcggcctcta tttcttgctg
    17761 atgccaaagc tgggtgaaga agatcgccag cgccggatgt acggtttacc cttcgcgtta
    17821 attgccggtg gctgcgtcgg tttttatgac ggattctttg gcccggctgc cggatcgttt
    17881 tacgcgctgg ccttcgttac gctgtgcgga ttcaacctcg ccaaagccac ggctcacgcc
    17941 aaattactca acgcaacgtc aaacattggc ggtctgctgc tgtttattct cggcggcaaa
    18001 gtgatttggg cgacgggctt tgtcatgctg gtggggcagt tcctcggcgc gcgcatgggt
    18061 tcacggctgg tgttgagcaa aggacaaaag ctgatccgcc cgatgattgt tatcgtctcg
    18121 gcggtgatga gtgccaaatt actttacgat agccacggac aggagatcct ccactggttg
    18181 gggatgaact aatgaacagt acacaccact atgagcagtt gattgaaatt tttaatagct
    18241 gctttgccga tgaatttaat acccgtctga ttaaaggcga cgacgaaccg atctatcttc
    18301 ctgctgatgc ggaagtaccg tataaccgaa ttgtctttgc ccacggcttt tatgccagcg
    18361 caattcatga gatttcgcac tggtgtattg ccggaaaagc gcgccgtgag ctggttgatt
    18421 tcggctactg gtattgcccg gatgggcgtg atgcccaaac gcaaagccag tttgaagatg
    18481 ttgaagtgaa gccgcaggca ctcgactggt tgttctgtgt ggcggcggga tatccgttta
    18541 acgtcagttg cgacaatctg gaaggcgact tcgaaccgga tcgtgttgtg tttcaacggc
    18601 gggtacatgc gcaggtgatg gattatctga caaatggcat tcccgagcgt ccggcacgct
    18661 tcattaaagc actacaaaat tattatcaca cgccggaact tacggcggaa cagttcccgt
    18721 ggccggaagc gctcaactga agccggtgcc acgttttaca atgaggaaag aagatgatcg
    18781 cggagtttga atcacgcatt ctggcattaa tcgacggcat ggttgaccat gccagtgatg
    18841 atgagttgtt tgccagtggg tatttgcgtg gccacctgac attagccatc gcagaactgg
    18901 aaagtggtga tgaccactcc gctcaggcgg tgcatacgac cgttagccag agtctggaaa
    18961 aagccattgg tgccggtgaa ttgtcgccgc gtgaccaggc gctggtgacc gatatgtggg
    19021 aaaacctgtt tcagcaggcg tcacagcagt aagttgcggc gtctggagca ccatgcctga
    19081 tgcgacgctt atcgcgtctt atcaggccta cacgtcacac gtaggtcgga taaggcgttc
    19141 acgccgcatc cggcaaacac tgcgagagca gattaccccg ccttaaccgc tttacccttc
    19201 aacaatttcc gcacccataa ccgattcggg tttaacgccg tcagcgtact ggcatccatc
    19261 ggaatcggtt cgtcgctcat ctgcgccgcc agaatctcgg cacacagcgg ggcagaacac
    19321 aaaccgcgag aacctaaagc cgcaaacata aagagatcgt caaaaaccgg cgcgcttacc
    19381 gcctcatctt tctgctccgc caacgacgca tattccacga gtgttgcctc ataatcggga
    19441 acattgccta ccattggcag atgatcgcgg gtggcacaac gcacaccgca gcgcgcctct
    19501 ttatcactga catcaacctc ttttgcccac tgtgcctgcg ggaaacaatc aatcaaccgc
    19561 tggcgattct gctgctgatc gtcctcactg tacgccgtat cttcgctgcc gcgatgataa
    19621 ctggcaccaa tacaatgatg ttgattcgcc ggattttgtg gcgtgagata accgtcatag
    19681 cacagcacct gcttcagctc tgccagttcc ggcgttgtcg gaatatggct gacctgcccg
    19741 gcaaccgaat acaccgggag agtcgacgtt tggctgaaac ggctgatttg atgcccgttc
    19801 gccagtacca ctacgctgtg tgttgcttgc tgatctcctg caaaattcaa caaccaacag
    19861 tcatccttac gggataaatt ctgtaactga tattgataat aaatctgcaa accctgctgt
    19921 tgcgccagtt ccagcacatt acgggtcagt tctgctgggc acagccaacc accttgcgga
    19981 taagtaatgc cgctgcaatt tgtcgcaacg cccgtaattt gttcaaccgc atttgcctca
    20041 acggctacag ccagttctgc gggtaaatcc attgacaaca tctgtgcgat tttatgctgg
    20101 cttttctcat cccagcctaa ctgcgtgacg ccgcaccagt catgatcaaa tttaacgggt
    20161 aattggtcgt aaaaccgacg agcaaaagta aacgcattag agaaaaagcg gtttagcgcc
    20221 tcatcgtgtt tgcttaataa cggatacagc gccccctggc gattgccgga agcacccagc
    20281 gcgggggctt catccgcgca ataaagcgct acctgccagc cgcgccgtaa taacgccagc
    20341 gacaacaacg cgctggcaat accaccgccg ataatcgcta cttcccgttt gctgctgccc
    20401 gtacgattaa accacggcgt ggagcagggg agtggtaatg tctgttccat caccccacaa
    20461 agcatttccc gtttgcgccc aaagccctta cgtttttgca tcgtgaatcc ggcttcctgc
    20521 aaaccgcggc ggacaaaacc ggcagacgta aatgtcgcca gcgtgccgcc tggacgcgcc
    20581 aaccttgcca tggcgttaaa cagattttgc gtccacatat ccgggttttt agctggcgca
    20641 aagccgtcca gaaaccaggc atctactttt tgatttagcg aatcgtccag ttggctggtc
    20701 agttcgttaa tatcgccaaa ccataaatcc agcgtcacgc ggccttcatt gagcaataaa
    20761 cgatggcaac cgggcaaggg cattggccac tgcgcctgaa gttgttctgc ccacggagcc
    20821 agttccggcc agtgttgatg cgctaaggct aaatccgcac gggtgagggg aaatttctca
    20881 aaactaatga aatgtaagcg ttgtaattgc gcttgcggat gcgcttcgcg aaactgatca
    20941 aatgcctgcc atagcgtcag gaagtttaat ccggtgccga agccgctctc tgctaccaca
    21001 aacagaggat gtggatgctc aggaaagcgt acctctaatt ggttgcctcc cagaaaaaca
    21061 taacgcgtct cttccagccc gttatcgttg gaaaaataga catcgtcaaa atctcgggaa
    21121 acaggtgtac cctcagcatt aaattcgagg ttggcaggtt gtatggagta gtgtttcacg
    21181 taagttactc gtcttacagg cggtggctcg atcttagcga tgtgtgtaag gctgcgcaaa
    21241 tttctctatt aaatggctga tcggacttgt tcggcgtaca agtgtacgct attgtgcatt
    21301 cgaaacttac tctatgtgcg acttacagag gtattgaatg aaacgtgcag tgattactgg
    21361 cctgggcatt gtttccagca tcggtaataa ccagcaggaa gtcctggcat ctctgcgtga
    21421 aggacgttca gggatcactt tctctcagga gctgaaggat tccggcatgc gtagccacgt
    21481 ctggggcaac gtaaaactgg ataccactgg cctcattgac cgcaaagttg tgcgctttat
    21541 gagcgacgca tccatttatg cattcctttc tatggagcag gcaatcgcag atgcgggcct
    21601 ttctccggaa gcttaccaga ataacccgcg cgttggcctg attgcaggtt ctggcggcgg
    21661 ttccccgcgt ttccaggtat tcggcgctga cgcaatgcgc ggcccgcgcg gcctgaaagc
    21721 ggttggcccg tatgtggtca ccaaagcgat ggcatccggc gtttctgcct gcctcgccac
    21781 gccgtttaaa attcatggcg ttaactactc catcagctcc gcgtgtgcga cttccgcaca
    21841 ctgtatcggt aacgcagtag agcagatcca actgggcaaa caggacatcg tgtttgctgg
    21901 cggcggcgaa gagctgtgct gggaaatggc ctgcgaattc gacgcgatgg gtgcgctgtc
    21961 tactaaatac aacgacaccc cggaaaaagc ctcccgtact tacgacgctc accgtgacgg
    22021 tttcgttatc gctggcggcg gcggtatggt agtggttgaa gagctggaac acgcgctggc
    22081 gcgtggtgct cacatctatg ctgaaatcgt tggctacggc gcaacctctg atggtgcaga
    22141 catggttgct ccgtctggcg aaggcgcagt acgttgtatg cagatggcga tgcatggcgt
    22201 tgatacccca atcgattacc tgaactctca cggtacttcg actccggttg gcgacgtgaa
    22261 agagctggca gctatccgtg aagtgttcgg cgataagagc ccggcgattt ctgcaaccaa
    22321 agccatgacc ggtcactcac tgggcgctgc gggtgtgcag gaagctatct actctctgct
    22381 gatgctggaa cacggcttta tcgccccgag catcaacatt gaagagctgg acgagcaggc
    22441 tgcgggtctg aacatcgtga ccgaaacgac cgatcgcgaa ctgaccactg ttatgtctaa
    22501 cagcttcggc ttcggcggca ccaacgccac gctggtaatg cgcaagctga aagattaatt
    22561 cgcagtaggc cggagtaggt cggataagac gcgccagcgt cgcatccgac gttacgcgcc
    22621 aatgcggcct ccggcactaa cgcaaaaggg aacctgatgg ttcccttttt cacatcattg
    22681 acaatcgcca ccagttccag gcaaacttcc cgctttgtcg atttccttct gaaaagacgt
    22741 acgcgttaaa tcctgccaac gcaccgtaac cctgaaacca gagagatgag acggggatac
    22801 tcctcgcctt gcgctgcatt ctggagtaat gcatgactgc tgtaagccaa accgaaacac
    22861 gatcttctgc caatttttcg ctcttccgca tcgcttttgc ggtttttctc acctacatga
    22921 ccgtagggct gccgttgccg gttatcccgc tgtttgttca tcatgaactg ggctatggca
    22981 ataccatggt cggcattgcc gtcgggattc agtttctggc tacggtgctg acgcgtggct
    23041 atgccgggcg actggccgat caatatggcg caaaacgttc ggcgcttcag gggatgttag
    23101 cttgtggtct ggctggcggc gcgttgctgc tggcggcgat tttgcctgtc tccgcaccgt
    23161 tcaaatttgc cctgttggtc atcgggcgtt tgattcttgg gtttggtgaa agccagttac
    23221 tgacaggcgc tctgacctgg gggttaggca tcgtagggcc aaaacactct ggcaaagtga
    23281 tgtcatggaa tggaatggcg atttacggtg ccctcgctgt tggtgctccg cttggcctgt
    23341 tgattcatag ccattacggt tttgccgcac tggcgatcac cacaatggta ttacccttac
    23401 tggcgtgggc ctgtaacggc acagtgcgca aagtaccggc cctggcggga gaacgtccat
    23461 cgctgtggag cgttgtcggg tttatctgga aaccagggtt agggctggca ctacaaggcg
    23521 ttggttttgc ggttatcggg actttcgttt cgctctactt tgccagcaaa ggatgggcga
    23581 tggcgggctt tactcttacc gagtttggcg gcgcatttgt cgtgatgcgc gtcatgtttg
    23641 gctggatgcc ggaccgtttt ggcggcgtga aagtggcgat tgtctctctg cttgtagaaa
    23701 cggtgggctt gttgctgctc tggcaagccc caggtgcgtg ggtcgcatta gcgggcgcgg
    23761 cgttaaccgg agccggatgt tcgcttatct ttcctgcgct gggcgtggag gtggttaaac
    23821 gcgtcccctc acaagttcgc ggcaccgcac tgggcggtta cgccgcgttt caggatatcg
    23881 ccctcggcgt ctccgggccg ctggcgggaa tgctggcgac cacgtttggt tactcttcgg
    23941 tatttcttgc cggggcgatc tctgcggtgc tgggaattat tgtcacgata ctgtcatttc
    24001 gtcggggcta acgaaccagc cagaccagca tcaggataat cgcgactaac agtatccaca
    24061 gcgcgggacg cgttgccata ttttgcaacg tgtccatcat cggtggaaaa ggattaagca
    24121 gcggttgcat atcacgcgga tcaatccctt taacccgccg ttgataaagc tgattaagaa
    24181 tatcctcggc ctgggcagaa gaaagcgatg attgcgcaga aagaccatat ttttgctgga
    24241 tatacgccga taacgctgcc agttcactgg catctaaagg ttgttttagc gccatctgta
    24301 gtgattccag cgtcggcgta ttttgctggc ttaacgtctg gcgcgcctgc aaccaggtca
    24361 ccagatggtt aaacagtttc gctggaatta actcgccatc gttcaccccg gaaagttcca
    24421 gcatcgattg ccagatctgt ttgctgggtt cccccgttgc cgccgcaagt ttggtcacca
    24481 gctgttttag cgcattgtgc tccgccggta ataaaggacg gtcggtcgcc tcgcgctgct
    24541 gtggttgcgg aataaccatc ttcccttcct gcaacagggt gagaatggtt tttaattgct
    24601 ccggtgagag ctgattcagc ggcgtctgac caaagttatg acggatataa tccgtgaccg
    24661 cctgacgatt attccccaga cgtaaatact cccctaattg cgctaaaagc tggcgggcag
    24721 aatggctttt ttgcgcggcc agcagacgtt gcgccagatt atgctcagcg gcagggaagt
    24781 gacgcgaaag cagcggtgaa tctcccgaca gaccaatatc gtgcctgata cccgcccaca
    24841 gttctgctct ttgttgttgc gtcagtgagg tcactttcgt cattaagctt tccagcgaag
    24901 tacgttgctg actggataaa ggctgattgc ccgcgccaga cggcaggtta tctccctgac
    24961 ctggtggttg cccaggagga gggccggaaa taggttgtat cattacgtat ccttatacct
    25021 gaaatcttcg caagtatgcc tggccgcgag attatggcac acttgtccgg ttaactctcg
    25081 tctcatacag gtaacacaaa cgtgaaaatc cttgttgatg aaaatatgcc ttatgcccgc
    25141 gacttattta gccgtttggg tgaggtgacc gcggttcccg ggcgtccaat ccccgtcgct
    25201 caactggcag acgcggatgc gctgatggtg cgttcggtca cgaaagtgaa tgaatctttg
    25261 ctggcaggaa aacccattaa atttgttggc actgccactg cggggaccga ccatgtcgat
    25321 gaagcatggt tgaagcaggc gggaattggt ttttccgctg cacctggctg taatgcgatt
    25381 gcggtggtgg aatatgtttt ctcctcacta ctgatgcttg ccgaacgcga tggattttca
    25441 ctgcacgacc gtacggtggg gatcgtgggc gttggtaacg ttggacgtcg attgcaggcg
    25501 cgactggaag cgttagggat taaaacattg ctttgcgatc cgcctcgcgc tgaccgtggg
    25561 gatgagggtg atttccgctc gctggatgaa ttagttcagc atgcggatat tctgactttc
    25621 catacgccac tcttcaaaga tgggccgtac aaaacgctgc atctggcgga tgaaaaactg
    25681 atccgtagcc tgaagccggg agcgattctg attaacgcct gccgtggcgc agtcgtcgat
    25741 aatactgcgt tgctgacctg cctgaatgaa ggccagaagt taagcgtagt gctggatgtc
    25801 tgggaaggcg aaccggaact taacgtggag ttgctgacaa aagtggatat cggcacgccg
    25861 catatcgcag gttataccct ggaaggtaaa gcacgcggta ctacgcaagt gtttgaagcc
    25921 tatagcaagt ttattgggca tgaacagcac gttgcgctgg atacattact gcccgcgcca
    25981 gagtttggtc gcattacgct gcatggcccg ctcgatcaac cgacgctgaa aaggctggtg
    26041 catttggtgt atgatgtgcg ccgcgatgac gcaccgctgc gtaaagtcgc cgggataccg
    26101 ggtgagttcg ataaactgcg caaaaactat cttgagcgcc gtgaatggtc atctctgtat
    26161 gtaatttgtg atgacgccag tgcggcatca ttgctgtgta aactgggttt taacgccgtt
    26221 catcatccgg cacgttaatc tcttcttcat gctctctgct gtaacattgg cagggagctt
    26281 tgctatttct ggagtaaacc accatgtctg aaggctggaa cattgccgtc ctgggcgcaa
    26341 ctggcgctgt gggcgaagcc ctgcttgaaa cgctggctga acgtcagttc ccggttgggg
    26401 aaatttatgc actggcacgt aacgaaagcg caggcgaaca actgcgcttt ggtggtaaaa
    26461 caatcactgt gcaggatgcc gctgaattcg actggacgca ggcgcagctg gcattttttg
    26521 tcgcaggcaa agaagctacc gctgcctggg ttgaagaagc gaccaactca ggttgcctgg
    26581 tgatcgacag cagcggactg tttgctctcg aacccgacgt accgctggtg gtgccggaag
    26641 taaacccgtt tgtactgacc gattaccgca accggaatgt catcgccgta ccagacagtc
    26701 tgaccagcca gctgctggcg gcactgaaac cgttaatcga tcagggcggt ttgtcgcgta
    26761 tcagtgttac cagcctgatt tcagcctccg cccagggcaa aaaagcggtc gatgcgttag
    26821 cggggcagag tgcgaaattg ctcaacggca ttccgattga cgaagaagat ttcttcggtc
    26881 gtcaactggc gttcaatatg ctgccgttgt tgccggatag cgaaggtagc gtgcgtgaag
    26941 aacgtcgtat cgttgacgaa gtacgcaaaa tcctgcagga cgaagggctg atgatttcgg
    27001 caagcgtcgt ccaggcaccg gtattctacg gtcatgccca gatggtcaac tttgaagcac
    27061 tgcgtccgct ggcggcagaa gaagcgcgtg atgcgtttgc tcagggcgaa gatattgtgc
    27121 tctctgaaga gaacgaattc ccgactcagg taggggatgc ttcgggtacg ccgcatcttt
    27181 ctgttggctg cgtgcgtaat gactacggta tgccggagca agtccagttc tggtcggtgg
    27241 ccgataacgt tcgctttggc ggcgcgctga tggcagtaaa aatcgccgag aaactggtgc
    27301 aggagtatct gtactaatgt ccgaccagca acaactgcca gtttataaaa ttgcgctggg
    27361 cattgagtac gacggcagta agtattacgg ctggcaacgg cagaatgaag tccgcagtgt
    27421 gcaggagaag ctggaaaagg cgctctccca ggtggcgaac gaacccatca ccgtcttctg
    27481 cgccgggcgt actgacgcag gggtacacgg taccgggcag gtggtgcatt tcgaaacaac
    27541 cgcgcagcgc aaagacgcgg cgtggacctt gggcgtaaat gcgaatttac ctggtgacat
    27601 cgccgtgcgt tgggttaaag ctgtacctga tgattttcat gcccgattta gcgccacggc
    27661 tcgccgttat cgctacatca tctacaatca tcggctgcgc ccggcggtat tgagtaaagg
    27721 ggtaacccat ttttacgaac cgctggacgc tgaacggatg catcgggctg cgcaatgctt
    27781 gctgggcgag aatgatttca cctcgttccg tgcggtgcag tgccagtccc gaaccccgtg
    27841 gcgcaacgtt atgcatatta atgtcacgcg tcacggtcct tatgtggtgg tagatatcaa
    27901 agcgaatgcc tttgtacatc atatggtcag gaatattgtc ggcagcctga tggaagtcgg
    27961 tgcccacaac cagccggaga gatggatagc agagttgctg gcggcaaagg acagaacgct
    28021 ggcggcagca acggcaaaag cggaagggct gtatctggtc gcggtggatt accctgaccg
    28081 gtatgatctt ccaaaaccgc caatgggccc gctatttctg gcggactaac gagaataatg
    28141 ctcggaacat ttcaggctgt agccaacgta gttacagcct gaaagatgac gagtacaagg
    28201 cataggcaaa tatggacctg atttatttcc tcatcgattt tatcctgcac attgacgtgc
    28261 atctggcaga gctggtcgcg gagtacggcg tctgggttta tgccattctg tttttgattc
    28321 tgttctgcga aacggggctg gtggtaacgc cattcctgcc tggtgattcg ctgctgtttg
    28381 ttgctggtgc attggcgtcg ctggaaacta acgatctcaa tgtccatatg atggtggtgc
    28441 ttatgttgat tgccgctatt gtcggtgatg cagtgaacta taccattgga cggttatttg
    28501 gtgacaaact gttcagtaac ccggattcga aaatctttcg tcgcagttat cttgataaga
    28561 ctcatcagtt ttacgagaga catggcggca aaacgattat tctcgcccgt tttgtaccga
    28621 tcgtcagaac gttcgcgccg tttgttgcgg gaatgggcca catgtcgtac cgtcatttcg
    28681 ccgcttataa cgtgatcggc gcactgttgt gggtactgat ttttacctac gcgggttatt
    28741 tcttcggcac gctccccttc attcagagca accttaagct gatgattgtt ggcattattt
    28801 ttgtttcaat attgcctggg gttttcgaat ttattcgtca caaacgcgct gcggcacgcg
    28861 ccgcaaaata aaaagtaact ccgcggttcg accacttttt tatccaaagt ttcgggctgt
    28921 tatgttttaa tgtgcaacat tcatggtctg ttgggggcaa aaatggcatt atgcgtcccc
    28981 aaagataaaa ctggcatcga accaggttca gacagaaagg tccctaatga gctggattga
    29041 acgaattaaa agcaacatta ctcccacccg caaggcgagc attcctgaag gggtgtggac
    29101 taagtgtgat agctgcggtc aggttttata ccgcgctgag ctggaacgta atcttgaggt
    29161 ctgtccgaag tgtgaccatc acatgcgtat gacagcgcgt aatcgcctgc atagcctgtt
    29221 agatgaagga agccttgtgg agctgggtag cgagcttgag ccgaaagatg tgctgaagtt
    29281 tcgtgactcc aagaagtata aagaccgtct ggcttctgcg cagaaagaaa ccggcgaaaa
    29341 agatgcgctg gtggtgatga aaggcactct gtatggaatg ccggttgtcg ctgcggcatt
    29401 cgagttcgcc tttatgggcg gttcaatggg gtctgttgtg ggtgcacgtt tcgtgcgtgc
    29461 cgttgagcag gcgctggaag ataactgccc gctgatctgc ttctccgcct ctggtggcgc
    29521 acgtatgcag gaagcactga tgtcgctgat gcagatggcg aaaacctctg cggcactggc
    29581 aaaaatgcag gagcgcggct tgccgtacat ctccgtgctg accgacccga cgatgggcgg
    29641 tgtttctgca agtttcgcca tgctgggcga tctcaacatc gctgaaccga aagcgttaat
    29701 tggctttgcc ggtccgcgtg ttatcgaaca gaccgttcgc gaaaaactgc cgccaggatt
    29761 ccagcgcagt gaattcctga tcgagaaagg cgccatcgac atgatcgtcc atcgtccgga
    29821 aatgcgcctg aaactggcga gcattctggc gaagttgatg aatctgccag cgccgaatcc
    29881 tgaagcgccg cgtgaaggcg tagtggtacc cccggtaccg gatcaggaac ctgaggcctg
    29941 ataactgata agggcagggc cactggctct gcccttttgc tattctcacc gtaacgaatc
    30001 agcggatacc atgattatca aacgcactcc tcaagccgcg tcgcctctgg cttcgtggct
    30061 ttcttatctg gaaaacctgc acagtaaaac tatcgatctc ggccttgagc gcgtgagcca
    30121 ggtcgcggcg cgtcttggcg tcctgaaacc agcgccattt gtgtttaccg ttgcgggtac
    30181 gaatggcaaa ggcaccacct gccgtacgct ggagtcgatt ctgatggcgg cagggtacaa
    30241 agtgggcgtc tacagttcac cgcatctggt gcgttatacc gagcgcgtac gtgtgcaggg
    30301 gcaggaattg ccggaatcgg cccacaccgc ctcttttgcg gagattgaat cggcacgcgg
    30361 tgatatttcc ctgacctatt tcgagtacgg tacgctgtcg gcgttgtggc tgttcaaaca
    30421 ggcacaactt gacgtagtga ttctggaagt agggctgggc ggtcgtctgg acgcaaccaa
    30481 tattgtcgat gccgatgtgg ctgtagtaac cagcattgcg ctggatcata ccgactggct
    30541 gggaccagat cgcgaaagta ttggtcgcga gaaagcaggc atctttcgca gcgcaaaacc
    30601 ggcaattgtc ggtgagccgg aaatgccttc taccattgct gatgtggcgc aggaaaaagg
    30661 tgcactgtta caacgtcggg gcgttgagtg gaactattcc gtcaccgatc atgactgggc
    30721 gtttagcgat gctcacggca cgctggaaaa tctgccgttg ccgcttgtcc cgcaaccgaa
    30781 tgccgcaaca gcgctggcgg cactgcgtgc cagcgggctg gaagtcagtg aaaatgccat
    30841 tcgcgacggg attgccagcg caattttgcc gggacgtttc cagattgtga gcgagtcgcc
    30901 acgcgttatt tttgatgtcg cgcataatcc acatgcggcg gaatatctca ccgggcgtat
    30961 gaaagcgcta ccgaaaaacg ggcgcgtgct ggcggttatc ggtatgctac atgataaaga
    31021 tattgccgga actctggcct ggttgaaaag cgtggttgat gactggtatt gtgcgccact
    31081 ggaagggccg cgcggtgcca cggcagaaca actgcttgag catttgggta acggcaaatc
    31141 atttgatagc gttgcgcagg catgggatgc cgcaatggcg gacgctaaag cggaagacac
    31201 cgtgctggtg tgtggttctt tccacacggt cgcacatgtc atggaagtga ttgacgcgag
    31261 gagaagcggt ggcaagtaag tttcagaatc ggttagtggg cacgatcgtg ctggtggcgc
    31321 tgggggtgat tgtacttcca gggctgctgg acgggcagaa aaaacattat caggatgagt
    31381 ttgcggctat cccgctggtt ccaaaagcgg gcgatcgcga tgagcctgat atgatgccag
    31441 ccgccaccca ggcgttgccg acgcagccgc cggaaggcgc agcggaagag gtgcgggcag
    31501 gtgatgccgc agcaccgtcg ctcgatccgg ccactattgc agccaataac accgagtttg
    31561 aaccggaacc tgcaccggtc gccccaccga agccgaaacc ggtggagccg cctaaaccga
    31621 aggttgaagc accacctgcg ccgaagccag aaccgaagcc ggtcgtggaa gaaaaagcag
    31681 cgccaacggg taaagcctat gttgtgcaac tgggtgcgct gaaaaatgcc gataaagtga
    31741 atgagattgt cggtaagctg cgcggtgccg gttatcgggt ttatacgtcg ccatccacgc
    31801 cagtgcaggg taaaattacc cgtattctgg ttgggccgga tgcctcgaaa gataagctga
    31861 aaggttcgct gggtgagttg aagcaacttt ctggcttaag tggcgtggta atgggctata
    31921 cgccgaatta atacagtctt gcctgatgcg acgctggcgc gtcttatcag gcctgggcag
    31981 tgttagaacc gtaggtcgga taaggcgttt acgccgcatc cgacacgcat tgcacgatgc
    32041 cgcaaaggca taaaaagtcg atggcgttga atattttttc agcgccattt ttattgatgc
    32101 gtgggaagga aatccctacg caaacgtttt ctttttctgt tagaatgcgc cccgaacagg
    32161 atgacagggc gtaaaatcgt gggacacata tggtctggat tgattacgcc ataatcgcgg
    32221 tgattgcttt ttcctctctg gttagcctga tccgcggctt tgttcgtgaa gcgttatcgc
    32281 tggtgacatg gggttgtgct ttctttgttg ccagtcatta ctacacttac ctgtcagtct
    32341 ggtttacggg ctttgaagac gaactggttc gaaatggaat tgccatcgcg gtactgttta
    32401 tcgctaccct gattgttggt gctatcgtga acttcgtgat aggccagttg gtggagaaaa
    32461 cggggttgtc aggcaccgat cgggtgctgg gcgtctgttt cggtgcgttg cgcggtgtgt
    32521 tgattgttgc cgccattctc ttctttctcg actcctttac cggggtgtcg aaaagcgaag
    32581 actggagcaa atcacagctg atcccgcagt tcagttttat catcagatgg ttttttgatt
    32641 atctgcaaag ctcgtcaagt ttcttgccca gagcgtaagt gctctgagat gtggcttaac
    32701 gaggaaaaag acgtatgtgc ggtattgtcg gtatcgccgg tgttatgccg gttaaccagt
    32761 cgatttatga tgccttaacg gtgcttcagc atcgcggtca ggatgccgcc ggcatcatca
    32821 ccatagatgc caataactgc ttccgtttgc gtaaagcgaa cgggctggtg agcgatgtat
    32881 ttgaagctcg ccatatgcag cgtttgcagg gcaatatggg cattggtcat gtgcgttacc
    32941 ctacggctgg cagctccagc gcctctgaag cgcagccgtt ttacgttaac tccccgtatg
    33001 gcattacgct tgcccacaac ggcaatctga ccaacgctca cgagttgcgt aaaaaactgt
    33061 ttgaagaaaa acgccgccac atcaacacca cttccgactc ggaaattctg cttaatatct
    33121 tcgctagcga gctggacaac ttccgccact acccgctgga agccgacaat attttcgctg
    33181 ccattgctgc cacaaaccgc ttaatccgcg gcgcatatgc ctgtgtggcg atgattatcg
    33241 gccacggtat ggttgctttc cgcgatccaa acgggattcg tccgctggta ctgggaaaac
    33301 gtgatattga cgagaaccgt acagaatata tggtcgcttc cgaaagcgta gcgctcgata
    33361 cgctgggctt tgatttcctg cgtgacgtcg cgccgggcga agcgatttac atcactgaag
    33421 aagggcagtt gtttacccgt caatgtgctg acaatccggt cagcaatccg tgcctgtttg
    33481 agtatgtata ctttgctcgc ccggactcgt tcatcgacaa aatttccgtt tacagcgcgc
    33541 gtgtgaatat gggcacgaaa ctgggcgaga aaattgcccg cgaatgggaa gatctggata
    33601 tcgacgtggt gatcccgatc ccagaaacct cgtgtgatat cgcgctggaa attgctcgta
    33661 ttctgggcaa accgtaccgc cagggcttcg ttaaaaaccg ctatgttggc cgcaccttta
    33721 tcatgccggg ccagcagctg cgtcgtaagt ccgtgcgccg taaactgaat gccaaccgcg
    33781 ccgagttccg cgataaaaac gtcctgctgg tcgacgactc catcgtccgt ggcaccactt
    33841 ctgagcagat tatcgagatg gcacgcgaag ccggagcgaa gaaagtgtac ctcgcttctg
    33901 cggcaccgga aattcgcttc ccgaacgttt atggtattga tatgccgagc gccacggaac
    33961 tgatcgctca cggtcgcgaa gttgatgaaa ttcgccagat catcggtgct gacgggttga
    34021 ttttccagga tctgaacgat ctgatcgacg ccgttcgcgc tgaaaatccg gatatccagc
    34081 agtttgaatg ctcggtgttc aacggcgtct acgtcaccaa agatgttgat cagggctacc
    34141 tcgatttcct cgatacgtta cgtaatgatg acgccaaagc agtgcaacgt cagaacgaag
    34201 tggaaaatct cgaaatgcat aacgaaggat gatgccttcg ctgagggtgc cggtctggca
    34261 ccctgacttg caactcccgc cgaaatacta caaagtctgc ctgcaagtct gacagggcaa
    34321 ctatttatga aacgactcat tgtaggcatc agcggtgcca gcggcgcgat ttatggcgtg
    34381 cgcttattac aggttctgcg cgatgtcaca gatatcgaaa cgcatctggt gatgagccag
    34441 gcggcgcgcc agaccttatc cctcgaaacg gatttttccc tgcgcgaagt gcaggcatta
    34501 gctgatgtca cgcacgatgc gcgcgatatt tccgccagca tctcttccgg ttctttccag
    34561 acgctgggga tggtgatttt accctgttca atcaaaaccc tttccggcat tgtccatagc
    34621 tatactgatg gcttactgac ccgtgcggca gatgtggtgc tgaaagagcg tcgcccgttg
    34681 gtgctctgcg tgcgtgaaac accattgcac ttaggccatc tgcgtttaat gactcaggcg
    34741 gcagaaatcg gtgcggtgat tatgcctccc gttccggcgt tttatcatcg cccgcaatcc
    34801 cttgatgatg tgataaatca gacggttaat cgtgttcttg accagtttgc aataaccctt
    34861 cctgaagatc tctttgcccg ctggcagggc gcataactcc gctgttgccc tgtttcaggg
    34921 caattttgca accgcgatca aatcctcgac attttgtttc tgccattcaa tcgaaacgct
    34981 gcgattcaac cgctatacct gctatcttca acttcaggac aataatgcaa cgtcttatta
    35041 acatatttaa cgttgaatgt tactgttgtc gtcaagatgg cataagacct gcatgaaaga
    35101 gcctgcaaac acacaacaca atacacaaca taaaaaagcc attttcactt gagggttatg
    35161 tatgaagaag tcgattctcg ctctgtcttt gttagtcggt ctctccgcag cggcttccag
    35221 ctatgcggcg ctaccggaga cggtacgtat cggaaccgat accacctatg cgccgttctc
    35281 gtcgaaagat gctaaaggtg attttgttgg ctttgatatc gatctcggta acgagatgtg
    35341 caaacggatg caggtgaaat gtacctgggt tgccagtgac tttgacgcgc tgatcccctc
    35401 actgaaagcg aaaaaaatcg acgccattat ttcgtcgctt tccattaccg ataaacgtca
    35461 gcaggagatt gccttctccg acaagctgta cgccgcagat tctcgtttga ttgctgccaa
    35521 aggttcaccg attcagccaa cgctggattc actgaaaggt aaacatgttg gtgtgctgca
    35581 gggatcaacc caggaagctt acgctaacga gacctggcgt agcaaaggcg tggatgtggt
    35641 ggcctatgcc aaccaggatt tggtctattc cgatctggct gcaggacgtc tggatgctgc
    35701 gttacaagat gaagttgctg ccagcgaagg attcctcaag caacctgctg gtaaagcttt
    35761 cgcctttgct ggctcatcag taaaagacaa aaaatacttc ggtgatggca ccggtgtagg
    35821 gctacgtaaa gatgatgctg aactgacggc tgccttcaat aaggcgcttg gcgagctgcg
    35881 tcaggacggc acctacgaca agatggcgaa aaagtatttc gactttaatg tctacggtga
    35941 ctgatacgtc gctgggaagc tgtacctgat ggaatgatca tcatggtgca cgccaggttt
    36001 gttgcactat cgtggtgcat tgaaatgcat acttaagcat ttttaatgaa aaataatacg
    36061 tctaacgggg cggaatattt tgccttgatg gtcaatttta tggcacgata agtgtaacaa
    36121 acctgtaaat attccctata aaaagactgt cagttgagga cattatgaaa aaactggtgc
    36181 tatcgctctc tctggttctg gccttctcca gcgcaactgc ggcatttgct gcgattccgc
    36241 aaaacatccg catcggtacc gacccgacct atgcgccatt tgaatcaaaa aattcacaag
    36301 gcgaactggt tggcttcgat atcgatctgg caaaggaatt atgcaaacgc atcaatacgc
    36361 aatgtacgtt tgtcgaaaat ccgctggatg cgttaatccc gtccttaaaa gcgaagaaga
    36421 ttgacgccat catgtcatcg ctttccatta cggaaaaacg tcagcaagaa atagccttca
    36481 ccgacaaact gtacgctgcc gattctcgtt tggtggtggc gaaaaattct gacattcagc
    36541 cgacagtcga gtcgctgaaa ggcaaacggg taggcgtatt gcagggcacc acccaggaga
    36601 cgttcggtaa tgaacattgg gcaccaaaag gcattgaaat cgtctcgtat caggggcagg
    36661 acaacattta ttctgacctg actgccggac gtattgatgc cgcgttccag gatgaggtcg
    36721 ctgccagcga aggtttcctc aaacaacctg tcggtaaaga ttacaaattc ggtggcccgt
    36781 ctgttaaaga tgaaaaactg tttggcgtag ggaccggcat gggcctgcgt aaagaagata
    36841 acgaactgcg cgaagcactg aacaaagcct ttgccgaaat gcgcgctgac ggtacttacg
    36901 agaaattagc gaaaaagtac ttcgattttg atgtttatgg tggctaatcg ccatcggtga
    36961 aagaaagccc gctcccttcg ggtaaccgga gggagacaag aggcggtaat tcaccacaca
    37021 cgacaggaca ggcagcatgt tgtatgggtt ttcaggtgtt attttacagg gtgcgctcgt
    37081 cacgctggag ctggctatca gctctgtagt gctcgctgta atcatcggtt taattggcgc
    37141 tggcggtaag ctctcgcaaa atcggctttc ggggctgatt ttcgaagggt acaccacgct
    37201 gattcgtggc gtgccggact tagtgttgat gctgctgatt ttctacggtt tgcagattgc
    37261 gctaaacacg gtgacggagg cgatgggcgt cgggcagatt gatatcgatc cgatggtcgc
    37321 tggtattatc actctcggtt ttatctacgg tgcttatttt accgaaacgt tccgtggtgc
    37381 ttttatggca gtgccgaaag gacatataga ggcggcgacg gcgttcggtt ttactcgtgg
    37441 gcaagtgttt cggcggatca tgtttccggc gatgatgcgt tacgccctgc caggcattgg
    37501 caacaactgg caggtgatcc tcaaatctac cgcactggtt tcgttactcg gcctggaaga
    37561 tgtggtcaaa gccacccaac tggcaggcaa aagtacctgg gaaccgttct atttcgccat
    37621 cgtctgtggc gtgatttacc tggttttcac cactgtttcc aatggtgtgc tgctgttcct
    37681 tgagcgccgc tactccgtgg gtgtgaagag ggctgacctg tgatcgaaat cttacatgaa
    37741 tactggaaac cgctgctgtg gaccgacggt tatcgcttta ctggtgtggc gatcactctg
    37801 tggctgctta ttttgtcggt agtgataggc ggagtcctgg cgctgtttct ggcgattggt
    37861 cgtgtctcca gtaataaata catccagttt ccaatctggt tatttaccta tatttttcgc
    37921 ggtacgccgc tgtatgttca gttgctggtg ttctattccg gcatgtacac gcttgagatt
    37981 gttaagggaa ccgaattcct taacgctttc ttccgcagtg gcctgaactg taccgtgctg
    38041 gcgctgacgc ttaacacctg cgcttacact accgagattt ttgctggggc aatccgttcg
    38101 gttccgcatg gggaaattga agccgccaga gcctatggct tctcgacttt taaaatgtat
    38161 cgttgcatta ttttgccttc tgcgctgcgt attgcgttac cggcatacag caacgaagtg
    38221 atcctgatgc tgcactctac tgcgctggcg tttacggcca ctgtgccgga tctgctgaaa
    38281 atagcccgcg atattaacgc cgccacgtat caacctttta ccgccttcgg cattgccgcg
    38341 gtgctctatt taatcatctc ttatgtcctg atcagcctct ttcgcagagc ggaaaaacgc
    38401 tggttgcagc atgtgaaacc ttcttcaacg cactgagaat acgatgtccg agaataaatt
    38461 aaacgttatc gatttgcaca aacgctacgg cgaacatgaa gtgctgaaag gggtctcact
    38521 gcaagcgaat gccggagatg taataagcat catcggatcg tcgggatcgg ggaaaagtac
    38581 ctttctgcgc tgcattaact tcctcgaaaa accgagtgaa gggtcgatcg tggtcaatgg
    38641 ccagacgatc aatctggtgc gcgacaaaga cggtcaactc aaagtcgccg ataaaaatca
    38701 actgcgctta ctgcgcacac gcctgacgat ggtattccag cacttcaatc tctggagcca
    38761 tatgacggtg ctggaaaacg tcatggaagc gccgattcag gtgttgggcc tgagcaagca
    38821 ggaagcgcgc gagcgggcgg tgaagtatct ggcgaaagtc gggatagacg aacgtgcgca
    38881 ggggaaatat ccggtgcatc tttccggcgg tcagcaacag cgtgtttcta tcgcgcgggc
    38941 gctggcgatg gaaccggaag ttttactgtt tgatgaacct acctcggcgc tcgatcctga
    39001 actggtaggc gaagtgttac gtattatgca gcaactggca gaagagggga aaaccatggt
    39061 ggtagtgact cacgaaatgg gctttgctcg ccatgtttct actcacgtca ttttcctcca
    39121 tcaggggaaa atagaagaag agggcgcgcc ggagcagtta tttggcaacc cgcaaagccc
    39181 tcgtctgcaa cagttcctta agggatcgct gaaataattt cctctccggt ggctaacgct
    39241 gccggagtat tttcatgggg aatttgcgag tcagcttccg ctgaaatgta aaagttgcag
    39301 tccgctaaaa atacttctga attcgctgtc caaagataaa aactgcctgc tgcattgggt
    39361 gttaatcgtg gttattgtcg cgtcatctta aatttgaggg gtgacggaac gccatgacaa
    39421 tatcgacaac ttccacgccg catgatgcgg tatttaaatc ttttttacgc catccag
//
LOCUS       DAJTZL010000015        39407 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.015, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000015 DAJTZL010000000
VERSION     DAJTZL010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39407
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.015"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..266
                     /locus_tag="OO544_000699"
     CDS             <1..266
                     /locus_tag="OO544_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HCR6766377.1"
                     /translation="TKAEAPAAQSKAAVPAKASDEEGTRVSINNASAEELARAMNGVG
                     LKKAQAIVSYREEYGPFKTVEDLKQVPGMGNSLVERNLAVLTL"
     gene            360..758
                     /gene="fadM"
                     /locus_tag="OO544_000700"
     CDS             360..758
                     /gene="fadM"
                     /locus_tag="OO544_000700"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HCR6766378.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(810..1505)
                     /gene="queC"
                     /locus_tag="OO544_000701"
     CDS             complement(810..1505)
                     /gene="queC"
                     /locus_tag="OO544_000701"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HCR6766379.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(1570..3270)
                     /locus_tag="OO544_000702"
     CDS             complement(1570..3270)
                     /locus_tag="OO544_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HCR6766380.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEQALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHRETSTDGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPHWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            3370..4188
                     /gene="cof"
                     /locus_tag="OO544_000703"
     CDS             3370..4188
                     /gene="cof"
                     /locus_tag="OO544_000703"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HCR6766381.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            4340..4798
                     /gene="decR"
                     /locus_tag="OO544_000704"
     CDS             4340..4798
                     /gene="decR"
                     /locus_tag="OO544_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HCR6766382.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            4828..6600
                     /locus_tag="OO544_000705"
     CDS             4828..6600
                     /locus_tag="OO544_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HCR6766383.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGLILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDCVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDVLAQQSGWYRD
                     MYRYQQLEAALDDAPENHEEAVDA"
     gene            6593..8374
                     /locus_tag="OO544_000706"
     CDS             6593..8374
                     /locus_tag="OO544_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HCR6766384.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDY
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSSNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            8555..8893
                     /gene="glnK"
                     /locus_tag="OO544_000707"
     CDS             8555..8893
                     /gene="glnK"
                     /locus_tag="OO544_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HCR6766385.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            8923..10209
                     /gene="amtB"
                     /locus_tag="OO544_000708"
     CDS             8923..10209
                     /gene="amtB"
                     /locus_tag="OO544_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HCR6766386.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(10258..11118)
                     /gene="tesB"
                     /locus_tag="OO544_000709"
     CDS             complement(10258..11118)
                     /gene="tesB"
                     /locus_tag="OO544_000709"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HCR6766387.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            11336..11908
                     /locus_tag="OO544_000710"
     CDS             11336..11908
                     /locus_tag="OO544_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HCR6766388.1"
                     /translation="MKLVHMASCLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINKGGTKADLTLVPMQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            complement(11939..12250)
                     /locus_tag="OO544_000711"
     CDS             complement(11939..12250)
                     /locus_tag="OO544_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HCR6766389.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            12413..12509
                     /gene="ffs"
                     /locus_tag="OO544_000712"
     ncRNA           12413..12509
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="OO544_000712"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            12629..12982
                     /locus_tag="OO544_000713"
     CDS             12629..12982
                     /locus_tag="OO544_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HCR6766390.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(13024..14572)
                     /gene="pdeB"
                     /locus_tag="OO544_000714"
                     /pseudo
     CDS             complement(13024..14572)
                     /gene="pdeB"
                     /locus_tag="OO544_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308537.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
     gene            complement(14736..15206)
                     /locus_tag="OO544_000715"
     CDS             complement(14736..15206)
                     /locus_tag="OO544_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HCR6766391.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(15322..15873)
                     /gene="maa"
                     /locus_tag="OO544_000716"
     CDS             complement(15322..15873)
                     /gene="maa"
                     /locus_tag="OO544_000716"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HCR6766392.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKDVPDNVVVGGNPARIIKKL"
     gene            complement(16044..16262)
                     /gene="hha"
                     /locus_tag="OO544_000717"
     CDS             complement(16044..16262)
                     /gene="hha"
                     /locus_tag="OO544_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HCR6766393.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(16288..16662)
                     /gene="tomB"
                     /locus_tag="OO544_000718"
     CDS             complement(16288..16662)
                     /gene="tomB"
                     /locus_tag="OO544_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HCR6766394.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIETFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            complement(17208..20357)
                     /locus_tag="OO544_000719"
     CDS             complement(17208..20357)
                     /locus_tag="OO544_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HCR6766395.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTSLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(20380..21573)
                     /gene="acrA"
                     /locus_tag="OO544_000720"
     CDS             complement(20380..21573)
                     /gene="acrA"
                     /locus_tag="OO544_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HCR6766396.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYLDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            21715..22362
                     /gene="acrR"
                     /locus_tag="OO544_000721"
     CDS             21715..22362
                     /gene="acrR"
                     /locus_tag="OO544_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HCR6766397.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            22490..25852
                     /gene="mscK"
                     /locus_tag="OO544_000722"
     CDS             22490..25852
                     /gene="mscK"
                     /locus_tag="OO544_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HCR6766398.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(26064..26225)
                     /gene="rsmS"
                     /locus_tag="OO544_000723"
     CDS             complement(26064..26225)
                     /gene="rsmS"
                     /locus_tag="OO544_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HCR6766399.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LTRDDKAEK"
     gene            complement(26239..26766)
                     /gene="priC"
                     /locus_tag="OO544_000724"
     CDS             complement(26239..26766)
                     /gene="priC"
                     /locus_tag="OO544_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HCR6766400.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALCHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            26836..27213
                     /locus_tag="OO544_000725"
     CDS             26836..27213
                     /locus_tag="OO544_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HCR6766401.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            27366..27917
                     /gene="apt"
                     /locus_tag="OO544_000726"
     CDS             27366..27917
                     /gene="apt"
                     /locus_tag="OO544_000726"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HCR6766402.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            28046..29971
                     /gene="dnaX"
                     /locus_tag="OO544_000727"
     CDS             28046..29971
                     /gene="dnaX"
                     /locus_tag="OO544_000727"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HCR6766403.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVVPTAVMTPTQVPPQ
                     SAPQQAPTVPLPETTSQVLAARQQLQCVQGATKAKKSESAAATRARPVNNAALERLAS
                     VTDRVQARPVPSALEKASAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPELA
                     AKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNRGA
                     QQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQT
                     LRRFFDAELDEESIRPI"
     misc_feature    29304..29368
                     /gene="dnaX"
                     /locus_tag="OO544_000727"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            30024..30353
                     /locus_tag="OO544_000728"
     CDS             30024..30353
                     /locus_tag="OO544_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HCR6766404.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            30353..30958
                     /gene="recR"
                     /locus_tag="OO544_000729"
     CDS             30353..30958
                     /gene="recR"
                     /locus_tag="OO544_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HCR6766405.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            31068..32942
                     /gene="htpG"
                     /locus_tag="OO544_000730"
     CDS             31068..32942
                     /gene="htpG"
                     /locus_tag="OO544_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HCR6766406.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTHTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            33123..33767
                     /gene="adk"
                     /locus_tag="OO544_000731"
     CDS             33123..33767
                     /gene="adk"
                     /locus_tag="OO544_000731"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HCR6766407.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYFKEAEAGNTKYAKVDGTKSVAEVRADLEKILG"
     gene            33899..34861
                     /gene="hemH"
                     /locus_tag="OO544_000732"
     CDS             33899..34861
                     /gene="hemH"
                     /locus_tag="OO544_000732"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HCR6766408.1"
                     /translation="MRQTKIGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEGGSPLMVYSRQQQQALAQRLPETPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(34858..35817)
                     /gene="aes"
                     /locus_tag="OO544_000733"
     CDS             complement(34858..35817)
                     /gene="aes"
                     /locus_tag="OO544_000733"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HCR6766409.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPSWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYTLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKIAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            35969..37273
                     /gene="gsk"
                     /locus_tag="OO544_000734"
     CDS             35969..37273
                     /gene="gsk"
                     /locus_tag="OO544_000734"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HCR6766410.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(37406..39082)
                     /locus_tag="OO544_000735"
     CDS             complement(37406..39082)
                     /locus_tag="OO544_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kef family K(+) transporter"
                     /protein_id="HCR6766411.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKAIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
BASE COUNT         9527 a        10049 c        10417 g         9414 t
ORIGIN      
        1 aaaccaaagc ggaagctcct gcagcacaaa gtaaagcagc agtaccggcg aaagccagtg
       61 acgaagaagg cacccgggtc agcattaata atgccagcgc ggaagagcta gcccgcgcga
      121 tgaatggcgt tggcctgaag aaagcgcagg cgattgtcag ttatcgcgaa gagtacggtc
      181 cgtttaaaac ggtggaggat ctaaagcagg tgccggggat gggcaattcg ctggtggaac
      241 gtaatctggc ggtattaacc ctgtaattaa tttgcatagt ggcaattttt tgccagactg
      301 aagaggtcat accagttatg acctctgtac ttataacaac aacgtaaggt tattgcgcta
      361 tgcaaacaca aatcaaagtt cgtggatatc atctcgacgt ttaccagcac gtcaacaacg
      421 cccgctacct tgaatttctc gaagaagccc gctgggatgg gttggaaaat agcgacagtt
      481 ttcagtggat gacggcccat aacatcgcct tcgtcgtggt caatatcaat attaactatc
      541 gtcgcccagc ggtattaagc gacctgttaa ctattaccag tcagttgcag caattaaacg
      601 gtaaaagcgg catcttaagc caggtcatta cactggagcc ggaagggcag gtggtagcgg
      661 atgcgcttat tacgtttgtt tgtattgatc ttaaaacgca gaaagcatta gctctggaag
      721 gggaattgcg cgaaaagctg gagcagatgg ttaagtaaac gttttgtggt gccggatgct
      781 caagccgcat ccggcgacac ccggaataat tacctcaacc cggttttctg cttcatcgct
      841 gccatcaccg tcggtttatc ggccagataa tgattcaaac cgttggcgcg taaattacat
      901 gccgcacaat gaccgcaacc gtcgccttta atgccgttat agcaggtcaa cgtttcgtta
      961 cggactaaat ccagtttgcc gtaataatct gccagcgccc aggtttccgc tttatcaatc
     1021 cacatcagcg gcgtttcaaa acgaatatct ttcgccatgc ccaaactgac ggcatggttt
     1081 agtgctttca caaactcatc gcggcaatcc gggtagccgg agaaatccgt ttcacagacg
     1141 ccggtaatta cggcttctgc ttttacctga tacgcatata ttgccgccag cgtcaggaac
     1201 aaaatattac gcccagggac aaacgtattc gggatgccat cggcttcagg ttcataatca
     1261 ggcaccggaa tgctgtcacg tgtcaggctg ctgaccgcca gctcgttgag cagcgttaca
     1321 tcaagaacct tatgcgcgcg tgctcccagt ttcagcgcca gttcgcgtgc cacgtcgatt
     1381 tctgcgcgat gtcgctgacc gtaatcgaac gtcacgcaat ggacttcatc atattgttgt
     1441 aatgcctgca ccagacaggt ggtggaatcc tggcctccac tgaacacaac gacagcacgt
     1501 ttcatagata atcctgcttg aacaataaaa gcgttatggt aacgcctgcg attaacccgg
     1561 accagctatt cattgcgacg gcgcgggaag ccaggcttcg gtaaattcaa accagccgcg
     1621 cggtgtcagt cgcacaccgt tcacgccggg aggggcacta atgcgatagt gatagttgaa
     1681 cagcggcgtc agcgtcgcgt ctgccattaa ctggctaaaa accgctttca gggcattaaa
     1741 tcggttttcc tcatcaggca ttacttgcac cgcgtccagc gtcgattgca gatgtgcata
     1801 tgctggagcg tcgaaaacat gtggccatag tggatcgcaa cgtagccatt gctccagagt
     1861 atattccggt gcttcgccaa ttaatctgtc gcccatcatg aggtctgcgt gtgccagtag
     1921 ggtcgtgtcg tcccagtttt ttgcgttatg aaaaataatt gtgagttcac agccttccgc
     1981 tgccagtgtc gcctgtaggc gttctgccat ggtatgaagt tctatcggta ggtgataaac
     2041 cagcgtcaag gttttcggta gtttgacttc atccggtact tgccagtgcg gaatagtcca
     2101 gcctggcagt aatgcatgac tggcggtgat caggttttct ccgacttcta acgtttgtaa
     2161 taaaccggat tgatgaataa tggagatcac ttttcgcgcc tgccagaggg agagtcgggg
     2221 acttttgcgc aacgttaaat agcaaaaacc taaactgatg ccactactga cctggctgac
     2281 ccgttgcagc tcctccggtt tgccgatggt gatttgcacg ggatgccgac aactggttcc
     2341 caaatctttt tcgaaaagcg gcggagttat ccagtactca accgctttaa gcagcggatg
     2401 acgcaaatgg taataatcat ggctctccag gcgcaccagc tccgctgtga attgtgttaa
     2461 tcgaaaagga ccagtgccga tcagtgggaa ttgcggatgc gccagatggc tgcaatagct
     2521 cgccagccgg tgcgcaagcc agtaatcggg gcgatgtaaa aagaaggtca gacactgcgg
     2581 atgggtgact tcaatacgct tcacgctaat aaataattga tccagtgctg gcagttgtaa
     2641 cagcatcaat aatcgctggt gtaagtgtga ggcttttact gcatcgccgt tatgccagtg
     2701 tagggttgaa cgaagataaa agtcccagcg taacccgtca gtagaggttt ccctgtgatg
     2761 cgctaaatcg ccaatcgggc gctgagtatt attatcgaag cgggtcaggc cggaaaatat
     2821 ctgcccggcg agatgctgct cggcacggcc gggcaaaaag cctggttgta gcggttcgag
     2881 cgggcgatag tagggaatac gcaacgtggg tgtatcgttt tgccattgtc cgcccataaa
     2941 cggctgtaac agagtgcgca gctcacctgg ggccagttgc gccagctcca gcacatcttg
     3001 ctgctttccg gtttccagtg cctgttccat catcgcattg cgtagcgatt ccggcgtgac
     3061 cagaaagcgt aattgtccgc gctttccgcg tcctgactgc gcctgccact ccagccatcc
     3121 cgcctcctgt gcctgacgca acagcgtacg aacatggcgt tcgctgcaaa aacagcgttc
     3181 ggccagttcg ctgacggtga cggtttgcgg ctttccggct gaaggttgcc acagacgttg
     3241 atactggtta agacggttga gcaatcgcat ataaacccgg aacaatatta tttaactatt
     3301 cactattact tccgtatata tcaggtgata ctcaatctcc attaaccgtg tcacagagtg
     3361 gagaaagaaa tggctcgtct ggcagcattt gatatggatg gcactttatt gatgcctgac
     3421 catcatttag gtgagaaaac cctctctact ttggcgcgac tgcgtgaacg cgacattacc
     3481 ctcacttttg ccacggggcg tcatgcgctg gagatgcagc atattctcgg ggcgctatcg
     3541 ctggatgcat atttgattac cggcaacgga acgcgcgtgc attctctgga aggtgaactt
     3601 ttacatcgtg atgatttacc tgcggatgtc gcggagctgg tgctgtatca gcaatgggat
     3661 acccgagcca gcatgcatat cttcaatgac gacggttggt ttaccgggaa agagatccct
     3721 gcgttgttgc aggcatttgt ctatagcggt tttcgttatc agataatcga tgtcaaaaaa
     3781 atgccactcg gcagcgtcac caagatctgc ttctgtggcg atcacgacga tcttacacgc
     3841 ttgcagatcc agctatacga agcattaggc gagcgtgcac atttgtgttt ttccgccaca
     3901 gattgcctcg aagtgctgcc ggtgggctgc aataaaggcg ctgcattgac ggtgctgacc
     3961 caacatttag gtttatcgtt gcgcgattgc atggcctttg gtgatgcgat gaacgatcgc
     4021 gaaatgttag gcagcgtcgg tagcgggttt attatgggca atgcgatgcc gcaactgcgc
     4081 gcggagctcc cgcatttacc ggtgattgga cattgccgaa atcaggctgt ctctcactat
     4141 ttgacgcact ggctggacta tccacatcta ccttattccc ccgaataacg agatcccttc
     4201 cagcaccggg caattgcccg gtttttttgc gttgaatttg tcattttgtg ccgtggtgtt
     4261 taaaccgcac agaataaatt gtcgtgattt cacctttaaa atagaattaa aagagaaaaa
     4321 aattctccgt ggaagggcta tgttagataa aattgaccgt aagctgctgg ccttactgca
     4381 gcaggattgc accctctctt tgcaggcact ggctgaagcc gttaatctga caaccacccc
     4441 ttgctggaag cgcctgaaac ggctggagga cgacggtatc cttatcggca aagtcgccct
     4501 gctggatccg gaaaaaatag gcctcggcct gaccgccttt gtgctgataa aaacgcaaca
     4561 tcacagcagc gaatggtatt gccgctttgt cacggtggtt accgaaatgc cagaagtgct
     4621 ggggttctgg cgcatggctg gtgaatacga ttatctgatg cgcgtccagg ttgccgacat
     4681 gaaacgctac gacgagtttt ataagcgtct ggtaaacagc gtgccggggc tgtcggacgt
     4741 cacttccagc ttcgcgatgg aacagattaa atacaccact tctttaccca tcgaataaat
     4801 atccagaatc aggtcaggac acaacgcgtg cgattatttg ctcaattaag ctggtatttc
     4861 cgtcgggaat ggcgtcgcta tctcggggct gtcgccttgc ttgtcattat cgcgatgctg
     4921 caactggttc cgccaaaagt ggttggtatt gttgtcgatg gcgtgacaga acaacacttt
     4981 actaccgggc tgatcctgat gtggatcgcc accatggtgc tgattgccgt tgtggtttat
     5041 ctcctgcgtt atgtctggcg cgtattgctg tttggcgcgt cttaccaact ggctgttgaa
     5101 ctgcgtgaag attattaccg tcagctaagc cggcagcacc cggagtttta cctgcgtcat
     5161 cgcacgggtg atctcatggc tcgtgcgacc aatgacgtcg attgcgtcgt gtttgccgcc
     5221 ggagaagggg tgctgacgct ggtggattca ctggtaatgg gctgcgctgt gttgattatg
     5281 atgtctacgc aaattagctg gcagttgacc ttatttgccc tgttgccgat gccagtgatg
     5341 gcgatcatga ttaagcgcaa cggcgatgct ttgcatgaac gctttaagct ggcacaggcg
     5401 gcgttttcca gtcttaatga ccgcacccag gaaagcctca ccagtatccg catgatcaaa
     5461 gcctttggtc tggaagatcg ccagtcggcg ttatttgccg cggatgccga agataccggc
     5521 aaaaaaaaca tgcgggtggc gcgtattgat gctcgtttcg acccgaccat ctatatcgcg
     5581 attggtatgg cgaacttgct ggcgattggc ggcggtagtt ggatggtggt gcagggcagt
     5641 ttaacgctgg gccagctcac cagttttatg atgtatttag gtctgatgat ttggccaatg
     5701 ctggcgctgg catggatgtt taacattgtg gaacgtggta gtgctgcgta cagccgtatt
     5761 cgtgcgatgc tggcggaagc gccggtggtg aacgatggta gcgaacccgt gccggaaggg
     5821 cgtggcgaac ttgatgtaaa tattcaccag ttcacgtatc cgcagactga ccatcctgcg
     5881 ctggaaaacg tcaatttcgc cctgaaaccc ggtcagatgc tgggtatctg cgggccgact
     5941 ggttccggca aaagtaccct gttgtcgctc attcagcgtc atttcgacgt tagcgagggg
     6001 gatattcgct ttcatgatat tcctctgacg aagttacaac tcgatagctg gcgtagccgc
     6061 ctggcggtgg tcagccagac gccattcctt ttttctgaca ccgtggcaaa taacatcgcg
     6121 ctgggttgcc cgaatgccac ccagcaagag attgagcatg tcgcgcggtt agccagcgta
     6181 catgacgata ttttgcgttt accgcaaggt tacgatacag aggtgggcga gcgcggcgtg
     6241 atgctttccg gcgggcaaaa acagcgtatc tctattgctc gtgcgttatt agtcaacgcg
     6301 gaaatcctca tccttgatga tgcgctttcg gcggtggacg gacgcacaga gcaccagatc
     6361 ctgcataacc tgcgtcagtg gggacaggga agaacggtaa tcatcagtgc ccatcgcctt
     6421 tcggcactga cggaagccag tgaaattatt gtgatgcagc acggacatat cgcccagcgt
     6481 ggcaatcatg atgtgctggc gcaacaaagc ggctggtatc gcgatatgta tcgctatcaa
     6541 caactggagg cggcgctcga cgacgctccg gaaaatcacg aggaggccgt cgatgcgtag
     6601 ttttagccaa ctgtggccga ctctcaagcg cctgttagcg tacggttcac cgtggcgtaa
     6661 accgctgggg attgcggtcc tgatgatgtg ggttgcggcg gcggcagaag tcagtgggcc
     6721 gctgcttatc agctatttta tcgacaatat ggtagcgaaa aataacctgc cgttgaaagt
     6781 ggttgcaggg ctggctgcgg cgtatgttgg gctacaactg tttgccgccg ggctacatta
     6841 cgcgcagtcg ctgctgttta atcgggcggc agtaggcgta gtgcaacagt tgcgtaccga
     6901 cgtgatggat gctgcgttac gccagccatt aagcgagttt gatacccaac ccgtcgggca
     6961 ggtgatttcc cgcgtcacta atgacactga agtgatccgc gatctctacg ttaccgtagt
     7021 ggcaactgtc ctgcgcagtg ccgcactggt gggcgcgatg ctggtggcga tgttcagcct
     7081 cgactggcga atggcgctgg tggcgataat gattttcccg gtagtgctgg tggtaatggt
     7141 gatatatcaa cgttacagca cgccgattgt ccgtcgtgtg cgcgcctatc tggcggatat
     7201 caacgacggc tttaacgaaa tcatcaatgg catgagcgtc atccagcagt tccgtcagca
     7261 ggcgcgattt ggcgaacgta tgggtgaggc cagtcgttca cactatatgg cgaggatgca
     7321 aaccctgcgc ctcgacggtt ttctgctgcg tccgctgctg agtctgtttt catcgctcat
     7381 tctttgtgga ttgttgatgc tgtttggctt ctccgccagc ggcaccattg aagtgggcgt
     7441 gctgtatgcc tttatcagct atcttgggcg acttaacgaa cccttaatcg aactgaccac
     7501 gcaacaggcg atgctgcaac aggctgttgt tgctggtgag cgcgtgtttg aactgatgga
     7561 cggaccgcgc cagcaatatg gcaatgatga ttacccgtta cagagtggca ccatcgaagt
     7621 cgataacgtg tcatttgctt atcgcgatga caatctggtg ctaaagaaca ttaatctctc
     7681 tgtgccttcg agtaactttg tggcgctggt cgggcatacc ggcagtggta aaagtaccct
     7741 cgccagttta ttgatgggct attacccgct aacggaaggt gagattcgcc ttgatggtcg
     7801 tccattaagt tcgctaagtc acagcgcgct gcgccagggc gtggcaatgg tgcagcaaga
     7861 tccggtggtg ctggcggata ccttcctcgc caacgtgacg ctggggcggg atatctccga
     7921 agaacgcgtc tggcaggcgc tggaaaccgt gcaactggcg gagctggcgc gtagcatgag
     7981 cgacggtatt tacacgccgc tgggcgagca ggggaataat ctctcagttg ggcaaaagca
     8041 actgctggca ctggcgcgcg tgctggtcga gacgccgcaa atcctgatcc ttgatgaggc
     8101 aaccgccagc attgactccg ggactgaaca ggcgattcaa catgctctgg cggcggtgcg
     8161 tgaacatacc acgctggtgg tgattgctca ccgcttatcg accattgttg atgccgacac
     8221 cattctggtg cttcatcgtg ggcaagccgt ggagcagggc actcaccagc aactgctggc
     8281 tgcccaggga cgctactggc agatgtatca actgcaactt gcgggcgaag agctggcagc
     8341 cagcgtgcgt gaagaggaat cattgagcgc ctgaatagcg caatatttca tcgttggtgc
     8401 aaaaatgtaa cgcactgtgc actgtcatag tgcgttttca ttttcaaact tcttaacttc
     8461 ctgctctctt tctcgttttt catttctggc acaccgcttg caataccttc ttcgtgtagc
     8521 agaaccatta ccgaattctg accggagggg atctatgaag ctggtgaccg tgataatcaa
     8581 accattcaag ctggaagacg ttcgtgaagc gttatcttcc attggtattc agggcctgac
     8641 cgtcaccgaa gtgaaaggtt tcgggcgtca gaaagggcat gccgagctgt accggggggc
     8701 ggaatacagc gtcaacttcc tgccaaaagt aaaaattgat gtggcgattg ctgatgacca
     8761 actcgatgaa gtgatcgata tcgtcagtaa ggcggcttac accggaaaaa ttggcgacgg
     8821 caaaatcttc gtcgctgaat tgcaacgcgt cattcgtatt cgtaccggcg aagccgacga
     8881 agcggcgctg taatctctgg cacacagcaa caggaacgaa aaatgaagat agcgacgata
     8941 aaaactgggc ttgcttcact ggcgatgctg ccgggactgg taatggctgc acctgcggtg
     9001 gccgataaag ccgacaatgc gtttatgatg atttgtactg cgctggtgct gtttatgact
     9061 attccgggga ttgccctgtt ttacggtggg ttgattcgcg gcaaaaacgt gctgtcgatg
     9121 ctgacgcagg tgacggtgac gtttgcactg gtctgtattc tctgggtggt ttacggttac
     9181 tcgctggcgt ttggtgaggg caacaacttc ttcggcaaca ttaactggtt gatgctaaaa
     9241 aacatcgaac tgacggcggt gatgggcagc atttatcagt atatccacgt ggcgtttcag
     9301 ggctcgtttg cctgcattac cgtcggcttg atcgttgggg cgctggcgga acgaatccgc
     9361 ttctcagctg tgttgatttt cgtggtggta tggctgacgc tctcttacat tccgattgcg
     9421 catatggtgt ggggcggtgg tttgctggct tctcacggtg cgctggattt cgcgggtggc
     9481 acggtggtgc atattaacgc cgcaatcgcc ggtctggtgg gcgcgtatct gataggcaaa
     9541 cgcgtgggct tcggcaaaga ggcgtttaaa ccgcacaacc tgccaatggt cttcaccggt
     9601 actgccattc tctatattgg ttggtttggc tttaacgccg ggtcagcggg cacggcgaat
     9661 gaaatcgcgg cactggcatt tgtgaatact gtggtcgcaa cggcggcggc aattcttggc
     9721 tggatcttcg gtgaatgggc gctgcgtggt aagccttctc tgctgggagc gtgttctggc
     9781 gcgattgccg gtctggtcgg cgtgacgcca gcgtgtggct acattggggt tggcggcgcg
     9841 ttgattatcg gcgtggtagc tggtctggcg ggcttgtggg gcgttactat gctcaaacgc
     9901 ttgctgcggg tggatgatcc ctgcgatgtc ttcggtgtgc acggcgtttg tggcattgtc
     9961 ggctgtatca tgactgggat ttttgccgcc agttcgctgg gtggcgtggg cttcgctgaa
    10021 ggtgtgacga tgggccatca gttgctggta cagctggaaa gcatcgccat tacgatcgtc
    10081 tggtccggtg tggtggcatt tattggctac aaactggcgg atctgacggt tggtctgcgt
    10141 gtgccggaag aacaggagcg agaagggctg gacgtcaaca gccacggcga gaatgcctat
    10201 aacgcgtaac aagcactgca aaaaacagcc ggacggtttt cacctccggc tattttttta
    10261 attgtgatta cgcatcaccc cttcctgaac ggtcgaggca accagcacgc cgtcctgagt
    10321 ataaaactca ccgcgcacaa agccgcgtgc gctggacgcc gaggtgctct ccacgctata
    10381 cagcagccat tcattcaaat taaacgggcg atgaaaccac atggaatggt caatggtggc
    10441 aatctgaatc cccggttcga gaaaaccgat gccgtgcggc tgtagagcta ccggcaggaa
    10501 gttaagatca gaagcgtaac cgagcagata ctgatgaacg cgcaggtcat ccggcacgct
    10561 accatttgcg cggatccaca cctgacgatg tggttctgcg acgtgacctt tcagtgggtt
    10621 atgaaactcc accggacgga cttccagcgg acgatcgcag atgaatttat ctttcagcac
    10681 tggcggcagc aggtgcgcca gcgattgggc gatttgtgtt tccgaaggca ggccatcagg
    10741 cgctggcgcg gacggcattg ttttttgatg ttcgaaaccc gcttcaggtg cctggaaaga
    10801 ggccgtcata taaaaaatcg gtttgccgtt ttgaatagca gcaacccggc gggcgctgaa
    10861 gctgttaccg tcacgcagcg tttcgacatc ataaataatc ggcttcttac tatcgccagg
    10921 gcgaagaaag tagctgtgaa acgaatgtac cagccgctct tcagggacgg tctcttttgc
    10981 agcatacaag gcctgaccca cgacctggcc gccaaacacc tggcgtaaac ctaaatcttc
    11041 actctggccg cgaaagagtc cttcctcaat tttttccaga tttaacaatg tcagtaaatt
    11101 ttttagcgcc tgactcatat aactctccag taactaagct gccgcagcaa gccaaagtga
    11161 gttgagtata acgcaaattt gccactggtc cgatgggtgc aatagtctga attacgggct
    11221 aattacaggc agaaatgcgt gatgtgtgcc acacttgttg acgttactat tttgttaacc
    11281 actcttccgg cgaggaaagt tagcccgctg gtgcattgat aataaggaga agtgaatgaa
    11341 actcgtgcac atggccagtt gtttagcggt tgcgattgcg ttggcggctt gcgcagataa
    11401 aagcgcggat attcagacgc cagcaccggc ggcaaatacg tctatttcag caacacaaca
    11461 acctgctatc cagcaaccga atgtctccgg taccgtttgg atccgtcaga aagtcgcact
    11521 gccgcctgat gctgtgctga ccgtgacact ttctgacgcg tcgttagccg atgcgccgtc
    11581 aaaagtgctg gcgcagaaag cggtgcgtac cgaaggtaaa cagtcaccat tcagctttgt
    11641 tctgcctttt aacccggcag atgttcagcc gaacgcgcgt attctgttga gtgcggcgat
    11701 taccgtgaat gacaaactgg tatttattac cgataccgtt cagccggtga tcaacaaggg
    11761 cggaactaaa gccgacctga cattggtgcc gatgcagcaa accgccgtgc cggttcaggc
    11821 cagcggtggt gcaacgacta ccgtaccttc gacttcacca actcaggtga atccgtcttc
    11881 ggcagttcca gctcctacgc aatattaagc gacgttaacc ctctccgccc ggagagggtt
    11941 agtagttcca gcgataacgc tgcaagtcga tttgcccgct tcccgatacc atcacacctt
    12001 ctgccagtaa tgcctgtcgc tgacgctgta aatccggtcc ggttagcgaa attgtgccgt
    12061 ggcgattaac cacccggtgc cagggtaagg tgctgccttc agggagacgc tttaacacac
    12121 cgcccacctg gcgcgcggcg cggggcgatc ccgccagttt cgccacatca ccgtaagtgg
    12181 tgacatagcc ttcgggaata gcggcgacga tttgccagac gcgttgggga aatgaatctt
    12241 ctttttccat cttttcttcc tgaggtaatt tttcagcata atctggaaaa acgcccgagt
    12301 gaagtcgcat tgcgcaagaa accagcatct ggcacgcgat gggttgcaat tagccggggc
    12361 agcagtgata atgcgcctgc gcgttggttc tcaacgctct caatgggggc tctgttggtt
    12421 ctcccgcaac gctactctgt ttaccaggtc aggtccggaa ggaagcagcc aaggcagatg
    12481 acgcgtgtgc cgggatgtag ctggcagggc ccccacccat ttctgcctcc caccgtttcg
    12541 tcaaaaaatc ccaacatagc taaactttaa ccacaactga cgtcgcaaga attgtctggc
    12601 tgcgcagtac gcttcggagg tatgtctgat gaagtatgtt gatggttttg tggttgccgt
    12661 tcctgccgat aaaaaggatg cctatcggga aatggccgct aaggctgcgc cattgtttaa
    12721 agagtttggc gcgcttcgta ttgtcgaatg ctgggccagc gatgtaccgg atggcaaagt
    12781 gaccgatttt cgtatggcgg tgaaagcgga agagaatgaa gaggttgtct ttagctggat
    12841 tgaataccct tcaaaagagg tccgcgacgc tgctaatcaa aagatgatgt cggacccacg
    12901 gatgaaagag ttcggcgagt ccatgccgtt tgacggcaag cgaatgatct atggcggatt
    12961 cgagtcaatc atcgacgaat aggtagcgtg acgggctgtg cggcgtgccg tcagcccaaa
    13021 aattcacaaa tgttgctcgg cccagcgtaa gaaatcttct ttcggtaatg ccttgctgta
    13081 gagccagccc tgaccgtagt gcacgccatg ctggcgtagc cactcttctt gtttactggt
    13141 ttcgattccc tccgctacca ttttcagttt cagtgttttt gccatttcga tgatatgcgg
    13201 cgtgacattt ttatattcca gcgcatcaac gaaagattta tcgatcttca gaatgtcgac
    13261 atccagatcc tgtaaataac ttaaacttga atatcccgta ccaaaatcat caagataaat
    13321 ttcatggccc gcctcccggt agcgagaaat tatcggggcg ctggttttcg gatcggcaaa
    13381 ctcgcgttca gtgagttcaa gcgcgatctg tctgggatta acctgattga ttgatcattt
    13441 cacgcagcaa ttgcgggatt ttttccgagg tgagcacggt ggattcaaga ttgatcgaaa
    13501 tatgctgctg tggatgctga cgcagccagt cgcccatatc ttcaaaggcg cttcttataa
    13561 tcagtagcgt caatggctca gaaaggcccg tttgctgtgc cagcggaata aaactatctg
    13621 gtgacaacca actaccgtct gtctgcggcc agcgcgccag tgcctcagca ccgacaattt
    13681 tgccattggc taaggagaca atcggctgat agtgcacgca aatatcacga ttttcgatag
    13741 catcctgcag ccgatgatgc ggtgactgga tacggcgcaa aatacgcagc acaaacatcg
    13801 ctgccagcag gccaatcacc aacccgagcg gtaaccaaat aaagacttgc cgatgccagc
    13861 ctttctgcaa cattttcgtt gaagcccagg tgatgatcga aatattcatc tccgggaagg
    13921 gcaggatatc gtaaatgatt ccattatttt cgatatgctc accgggtgtt ttttgtagcc
    13981 tggtaataat tccctgagca atttcatcgc tgctggttat gacaacgtta tgggcattgc
    14041 caataatggc ggcatcaatt tgccatgagc tatagggaat gacatcaata aaggaagcgg
    14101 ggtcgatcat gacgacataa tgtgccgttc ccatggcgac catgtaacgg ataatgccta
    14161 aatcgttatg cgatgttaac cagacacgat aaccatcttt cgaaatttta cctggctcgg
    14221 ggaaggtatc gggcggactt tcatgctcca gagacgaaca ctggggaacg ttgttatcga
    14281 tataaaccac ttcctgaata tagcgataac tgtaagatac ccgacgcatt tccatgagat
    14341 gggcttcgct acaggcagcg ccttgccatc tttccagctc ttgcagcgca tctttccctt
    14401 gtgtcgccac cttattggcc cgaatagcga cgcgggagga ataggtatcc agctcttcaa
    14461 taaacgatgt ttctacctgc tgatgggcca gccagatgct taagccgaca ggcagcaata
    14521 ctgaaagaat cagtactccc gaaataaggc cgaccagatg tcgtgttctc accagcatat
    14581 ccttattaac acagtgtagg gaaagtatat ctgattgtgg aaaaaggctg gcaggagata
    14641 aggaggtaga gctggtattt catgaagtga tgggtggaaa cgatcattgt catttgacca
    14701 agcgcagagc tttacgcctg gtatgtaacc ctaacttaag atgttgattc ggcgcgagcg
    14761 agggtaaaaa tatcgtaaaa agacagttca cctttacgga cgatcatttt ttgcagttgt
    14821 tccttatgaa catccgcgac acgcggagag ttcaagatgg cttcaaccag cgcggcaggt
    14881 acaaagcgcg tgttgtacca ttcgccgtta tagcaaacgc ggaaatccag cacgtcgata
    14941 tcttcatagc ggtagcgtgg gtagacatcg aaaaagaaga aaccattaag caggataaat
    15001 aatacaacca gtagccagac agagcctgac agcgtttcgg actgcaacat caccgccagg
    15061 gtggcaaaaa aagcaacgta cataccgata aacagccccg gatgtttacg gataaaactg
    15121 atactaaagc ggggtttgtt gtcgcgtttt tcgcgggtat tcagagactc aatcgtttcg
    15181 gtcagcaggc gttgtatttc ggtcatcgtt tgccttcgtg gtatctgcaa ctttccaggg
    15241 aacaccctat tttaacgtgg gaatagtaga ggggaagtaa cagatttacc acaattttgc
    15301 ataacagttg cgaaaaaccg attacaattt tttaattatt ctggctggat taccgcccac
    15361 gacaacgttg tccgggacat cttttgtgac aactgcacct gaggctacca cgacgttatc
    15421 accaatggtc acaccagggt taatgaccgc gcgtccgcca atccagacgt tattaccgat
    15481 ggtgacgggt ttccccagtt cagcaccgct attacgtgct acagggtcga tgggatgtgt
    15541 tgccgtgtag atatgaacgc ctggtgccaa catacagtta tcaccgatgc gaatagggca
    15601 gacatcaagc atcacgcaat cgaagttggc gaaaaaatta ttaccgagaa aaatgttata
    15661 gccatagtca cagcgaaacg ttggctcaat ataagcctct gtcacctgac cgaatagatc
    15721 agcgagaatt tgctggcgta atgtgtgctc ttccgccagg gaatgattgt atcggtgaat
    15781 aagctgacga gcgcgcaggc gatcgcgaga taacgtctca tctgccgagc gatacaactc
    15841 accagcaatc atcttttctt tttctgtgct cattactgac ctccggttgc tgaaaatgcg
    15901 gcaacacagt aagacgaaaa gtcagtgaag agaacgttcc cattacatct gtgtgataaa
    15961 gatcacatag ggtatgtctt catggtgaaa agtataaaat tcttaataaa cagccggtta
    16021 tagctccgaa agcgaattat ttattagcga ataaatttcc atactgagga agggatcttg
    16081 tcgtatagtt tattcatggt caattcggcg aggcggtgat ctgcggctga gtaaaatacc
    16141 gccagttcat tatctgataa ttcgtattta tttttctcga taacacgctc cagcgtgtca
    16201 attgtctggc aacgacgtaa acgcattaaa taatcggttt tcgttaaagg tttttcggac
    16261 atacttctac ccatggttgt aataattcta acaagataaa ctcgcaggat tctctttcgt
    16321 cgcattgaca aaacaacgga atagtcgatt acctgacttc cgccatttct gaagatcctg
    16381 catattaata ccataactac tgaacaacat aaaggtgtca tccagatatt cgtcgatctg
    16441 ctcaatgagc ttattgtctt cattatactt aattttgtaa ttaagtgcga aggtctcaat
    16501 atgctcaatc agttcattca actggaggtt gatcgccgag gttgggtcgt taacccagcc
    16561 atgattgctt tcttcaaggt ttgcaaggca gtcatgatac agggtttcac agagaaactt
    16621 aagctgtgcg atatcatgtc ttttgggtga gtattcatcc ataacgcgtc cccttcttag
    16681 cggttgaact aacggacacc tttcgggatg gaaaaaactt actgacctgg acttgccctc
    16741 tcgtttgtta gcttaactat aagccactct ttgcaggttt tcatcgcatt attacgaaaa
    16801 attacaatta ggaaggaatc gggacaggac tcactttttc atactagcac taacccgcag
    16861 caggtaaaag cagtctgctc gaactgttca taacatttca ggattatcgc cagatgaaat
    16921 tggtgacact aataccagga ttgctctgaa tatgacgtaa taaccgagga atgaataaag
    16981 aattaccgca aataattaag aatagcctct aaatgattat ggattattaa atctattagc
    17041 gctacttaat ataaattaac taacaatcag tattttttat gaattttatc cgtggttaat
    17101 actggttttc gtatgagatc ctgagttggt ggttcaatta ctccttaatg ttcgtaggtt
    17161 atgcataaaa aaggccgctt gcgcggcctt agtgattaca cgttgtatca atgatgatcg
    17221 acagtatggc tgtgctcgat atcttcattc ttgcggctaa agcggcggcg aaccaccaca
    17281 aagaataccg gaacgaagaa gattgccagt accgttgcgg tcaccatccc gcccattaca
    17341 ccggtaccta ctgcgttctg cgcgccggaa ccagcaccag tactgataac cagcggcata
    17401 acgccgagga taaacgccag cgaggtcatc aggatcggac gtaaacgcat ccgcactgca
    17461 tcaagcgtcg cttcaatcag acctttacct tctttatcca tcaagtcttt ggcgaattcg
    17521 acgataagta tcgcgttctt cgccgacaac ccaatggttg tgagcaggcc tacctggaag
    17581 taaacgtcat tggtcaggcc acggaaggtg gcagccagca acgcaccgat aacccccagc
    17641 ggaacgacca gcataacgga gaacggaatc gaccagctct cgtacagcgc cgccagacac
    17701 aggaatacga caatcaacga aatcgcgtac agtgaaggtg cctggttgcc ggagagacgt
    17761 tcctgatagg acatccccgt ccagtcatag ccaacaccgg taggcagttt gctcgccagt
    17821 tgttccatca gctccattgc ttcaccggta cttttacccg gtgccgcctg gcctaagatt
    17881 tccatggatg gcaggccgtt gtaacgttcc agacgcggcg aaccgtactc ccaacgagaa
    17941 gaggagaacg ccgagaatgg caccatctga ccatcagcag cacgaacata ccagtcgccg
    18001 atatcatccg gcagcatacg gtatttcgct tctgacatga cgtaaacttt cttcacacga
    18061 ccgcggtcga taaagtcgtt cacatagcta ccgccccatg cagcgcccag agaggtgtta
    18121 atgtcgttga tagaaacacc cagcgcctgc gctttttcct ggtcgatatc aatcttaaac
    18181 tgcggggtat cttccagacc gtttggacgt acgctggtca gcatatcagg gtgcttcgct
    18241 gcttctgcaa gcagctggtt acgcgcctga gtcagttttt cgtgaccaag gccagcctgg
    18301 tcaatcagct caaagtcaaa gccggttgca gtacccagtt ccacgattgc gggcaggtta
    18361 aaggcgaaaa ccatcgcatc tttgatttgc gagaaagcgc gtgttgcacg catggtaatc
    18421 gcttcaactt tgttttcttc gcccggacga tcggcccagt ccttcaagga aacgaacgca
    18481 ataccggtat tctgaccacg tcccgcaaag ccgaagccgt taacggcgaa caccgactca
    18541 acgttgttct tttctttggt cagatagtaa tgcgttacct cattgagcac tttctgcgta
    18601 cgttcctgcg ttgcacctgc tggcagctga accatggtca taaatacgcc ctggtcctca
    18661 tctggcaaga aggagcttgg cagacgcacg aacagatagg ccatgccgac cacgatgatc
    18721 agatacagca ccaggtaacg ccccgtactg cgcagaatac cgcctacgct gtcggtgtag
    18781 tggtgcgtgc tcttctcgaa catgcggtta aaccagccga agaagccttt tttaccttcc
    18841 ccgtgatcgc ctttggcaat cggtttcagc atggtggcac aaagagccgg agtcaggatc
    18901 aacgctacca gtaccgacag cgccattgct gaaacaatgg taatagagaa ctgacgatag
    18961 atagcaccag tagaaccgcc aaagaaggcc atcggtacga ataccgccga cagtaccatc
    19021 gcgataccga ccagagcgcc ctgaatctgc cccatcgact tacgggtagc ttcttttggc
    19081 ggcaaacctt cttccgccat aacacgctcc acgttttcta ccacaacgat agcgtcatcc
    19141 accaacaagc cgatggcgag caccatcccg aacattgtta gcgtgtttat cgagaagcca
    19201 aaggcggcaa ggacggcaaa ggtcccgagc aataccaccg gtacggcaat ggtcggaatc
    19261 aacgtcgcgc ggaagttctg caggaacaga tacataacca ggaacacgag gatgatcgct
    19321 tcgaccagcg ttttaaccac ttcgtgaata gagattttca cgaacggcgt ggtgtcgtac
    19381 gggtaaacaa ttttcagacc cgacgggaag aacggttcca tcttcgccag ttcagcacgg
    19441 attgccgcag cggtatccag cgcgtttgca ccggtcgcca gcttgatccc cagaccggaa
    19501 gccggttggc cgttaaactc tgcgatgatg tcgtagttct caccacccag ctcaatcttc
    19561 gccacatcac gcagcagtac gcgggaacca tcctgattca ctttcagcag gattttgccg
    19621 aactcttcag tagaggtcag acgcgtctga gcaataatag aggcgttaag ctgttggcct
    19681 ttcaccggcg gcgtaccacc gagctgaccc gccgcaacct gggcgttctg cgctttgatg
    19741 gcggtaatga catcaaccgg cgttagctgg aatttgttca gctcattcgg gttcatccag
    19801 atacgcatcg cgtactgtga accgaacaac tgaacatcac ccacgcccga cgtacggctg
    19861 atggcatctt tcatattcgc cgctacgtag tcagagatat cctcctgcgt catggtgcca
    19921 tcggtgttga taacgccgac aaccatcagg aagctgctgg atgatttctc aacgctcacc
    19981 ccttgctgct gaacttcttg cggcagcaac ggcatcgcca gctgcagttt gttctgcacc
    20041 tgaacctgcg cgatatccgc atcagtacca gactcaaagg tcagggtgat ctgcacggta
    20101 cccgtggagt cactgttaga ggacatgtac atcaggttat cgataccgtt catattctgt
    20161 tcgataacct gtgtcaccgt gtcctgcact gttttcgcat cagcgccggg gtaggaggcg
    20221 gagatcgtta ctgccggcgg tgcaatcgta ggatattgcg ccaccggcag tttgaggatc
    20281 gccagccccc ctgccaacat gatgataatg gcgatcaccc acgcaaaaat cgggcgatcg
    20341 ataaagaaat taggcatgtc ttaacggctc ctgtttaagt taagacttgg actgttcagg
    20401 ctgagcaccg cttgcggctt gctggttatt atcagcggta acttcttgtg cttttacctg
    20461 gacaccagga cgcactttct gcagcccact tattactacg cgatcgcctg ctttcagacc
    20521 ttctgtcacc agccacttat cgccaatagc ctggcttgca acgatcggac gggtttccac
    20581 tttgtcatcc gcgccaacta ccagtacggt ggcatcacca cgcggcgtac gggttacgcc
    20641 ctgttgcggg actaaaatag cgtttggatt aagcccttct tccagacgtg cgcgcacgaa
    20701 catacccggc agcagagtgt gatccgggtt cgggaagata gcgcgtaggg tgatagaccc
    20761 agtggtctga tcaacggtaa cgtcagagaa ttccagcgta ccgtcctgcg ggaacttaat
    20821 gccgtcactg gtgatcagcg acactttggc tttgccgttc tcttgtttca gcgtgccatt
    20881 cgccagttcc tgtttcaggc gcaggaagtc gttgctggac tgggtcacat caaggtagat
    20941 cggatcaagt tgctgcacgg ttgccagcgc agtcgcctga ccgttctgta ccaatgcgcc
    21001 ttccgtcacg ttcgacttac caatgcgacc gctaatcgga gaggtgactt tggtgtaagc
    21061 cagattgatc cgcgcagttt caacggcagc tttcgccgca gttaccgcag cattcgcctg
    21121 ttgcgcatca gccagagcct gatcgtactc ttgcttactg atgtactgag taccgagcag
    21181 tttctgataa cgattcaccg tcaattgcgc gatattggct gcagcctggg ctttcgccag
    21241 atcacctttc gcactgtcgt atgtcgcctg ataggtcgca ggatcaatct gatagagaga
    21301 gacaccagct tcgatgtcgc taccttcttt gaaattacgc ttcaggataa tcccgctaac
    21361 ttgaggacga acttctgcga tccggtaggc actggtgcga cccggaagct cggttgtgat
    21421 ctgcagaggt tcagttttga ctgttactac gccaacggcg ggcatctgct ggccaccttg
    21481 ttgggcctgt ttgtcgtcac atcctgttag ggctaagctg cctgagagca tcagaacgac
    21541 cgccagaggc gtaaaccctc tgtttttgtt catatgtaaa cctcgagtgt ccgatttcaa
    21601 attggtcaat ggtcaaaagt taataaaccc attgctgcgt ttatattatc gtcgtgctat
    21661 ggtacataca ttcacaaatg tatgtaaatc taacgcctgt aaattcacga acatatggca
    21721 cgaaaaacca aacaagaagc gcaagaaacg cgccaacaca tcctcgatgt ggctctacgt
    21781 cttttctcac agcagggggt atcatccacc tcgctgggcg agattgcaaa agcagctggc
    21841 gttacgcgcg gtgcaatcta ctggcatttt aaagacaagt cggatttgtt cagtgagatc
    21901 tgggaactgt cagaatccaa tattggtgaa ctagagcttg agtatcaggc aaaattccct
    21961 ggcgatccac tctcagtatt aagagagata ttaattcatg ttcttgaatc cacggtgaca
    22021 gaagaacgac gtcgattatt gatggagatt atattccaca aatgcgaatt tgtcggagaa
    22081 atggctgttg tgcaacaggc acaacgtaat ctctgtctgg aaagttatga ccgtatagaa
    22141 caaacgttaa aacattgtat tgaagcgaaa atgttgcctg cggatttaat gacgcgtcgg
    22201 gcagcaatta ttatgcgcgg ctatatttcc ggcctgatgg aaaactggct ctttgccccg
    22261 caatcttttg atcttaaaaa agaagcccgc gattacgtcg ccatcttact ggagatgtat
    22321 ctcctgtgcc ccacgcttcg taatcctgcc actaacgaat aaccctgaat ctgactccag
    22381 gatttttcct ggacattttc gtcgttgcta ttctggttca ctgcgtcgtg atattcttgc
    22441 ggtttgactt tttcaggtcg ttcttcaggt tcagaaacct tcattcatca tgactatgtt
    22501 ccagtattac aaacgatcac ggcattttgt tttttcagca tttattgctt ttgtttttgt
    22561 cttgttatgc cagaacacgg cgtttgcgcg ggcgtcatcg aatggtgatc tgccgacaaa
    22621 agcggacctg caggcgcaac ttgactcact aaataaacaa aaagatcttt ctgctcagga
    22681 caaactggtg cagcaggatc tgacagatac attagccacc ctcgataaaa tcgatcgcat
    22741 aaaagaagag acagttcagc tacggcaaaa agtcgctgaa gcgccagaaa aaatgcgcca
    22801 ggcgaccgcg gcgttaacag cacttagcga tgtcgataac gacgaagaaa cgcgcaaaat
    22861 tctgagtacg ctttcgttgc gccagctgga aactcgcgtt gcccaggcgc tggacgattt
    22921 gcaaaacgca caaaacgatc tggcgtctta taacagccag ctggtttcgt tacagacgca
    22981 gcccgaacgc gtgcaaaatg cgatgtataa cgcttcgcag cagctgcaac aaattcgcag
    23041 tcgtctggat gggactgatg tcggcgagac tgccttacgt cccagccaga aagtgttaat
    23101 gcaggctcag caggcgttgc tgaatgcgga gattgaccag cagcgtaaaa gcctggaagg
    23161 gaacaccgtc ttgcaggata ccttgcaaaa gcaacgtgat tacgtgacgg cgaacagcgc
    23221 ccgtctggag caccagttgc agctgttgca agaagcggta aacagcaagc gcctgacttt
    23281 aactgaaaaa acggcgcagg aagccgtctc cccggatgaa gccgcgcgta ttcaggctaa
    23341 tccgctggtg aaacaggaac tggaaattaa ccagcagtta agtcagcgtc tgattaccgc
    23401 gactgaaaac ggtaatcagt tgatgcagca aaacattaaa gtcaaaaact ggctggagcg
    23461 ggcgctgcaa tcggaacgca atattaaaga gcagattgcc gtcctgaagg gcagcctgct
    23521 gttgtctcgt atcctttacc agcaacaaca aacgctgccc tcggcggatg aactggaaaa
    23581 catgaccaac cgcatcgcgg atttgcgtct cgaacagttt gaagttaacc agcagcgtga
    23641 tgcactcttc cagagcgatg cgttcgtcaa caaactggaa gaaggtcaca ccaacgaagt
    23701 caacagcgaa gttcacgatg cgttattgca agtggttgat atgcgtcgcg aattgctgga
    23761 tcaactcaac aaacagttgg gtaaccagct gatgatggcc attaacctgc aaatcaacca
    23821 gcagcagtta atgagcgtat cgaaaaacct gaaatccatc ctgactcagc aaatcttttg
    23881 ggtgaacagt aaccgtccaa tggactggga ctggatcaaa gcgttcccgc aaagcctgaa
    23941 agatgaattt aagtcgatga aaatcacggt gaactgggaa aaagcctggc ctgccgtttt
    24001 tatcgctttc ctcgccggtt tgccgctgct gttgattgcc gggctgatcc actggcgtct
    24061 gggctggctg aaagcgtatc aacagaaact ggcttccgct gtgggttccc tgcgtaacga
    24121 tagccagctc aacacaccaa aagcgatcct tatcgacctg atccgcgcgc tgccggtgtg
    24181 cctgattatt ctcgcggttg gcctgattct gttgaccatg cagctcaaca tcagcgaact
    24241 gctatggtcg ttcagtaaaa aactggcgat tttctggctg gtgtttggcc tgtgctggaa
    24301 ggtactggag aaaaatggcg ttgccgtgcg tcacttcggc atgccggagc agcagaccag
    24361 ccactggcgt cggcaaattg tccgcatcag tctcgcgttg ctgcctatcc atttctggtc
    24421 tgtggtggca gaactttccc cgctgcatct gatggatgat gtgctggggc aggcgatgat
    24481 tttcttcaac ctgctgctga ttgccttcct ggtatggcca atgtgccgcg aaagctggcg
    24541 tgataaagag tcgcacacca tgcgactggt caccattacc gtgctgtcga ttatcccgat
    24601 tgcattgatg gtgctgactg ccacgggtta tttctacacc acgctgcgcc tctccgggcg
    24661 ttggattgaa accgtttatc tggtgatcat ctggaacttg ctctaccaga cggtattgcg
    24721 cggcttaagc gtagcggcgc ggcgtatcgc ctggcgtcgt gcgctggcgc gtcggcagaa
    24781 tctggtgaaa gagggcgcag aaggtgccga accgccggaa gaacccacca ttgcactgga
    24841 acaggttaac cagcagacgc tgcgtattac catgttgctg atgtttgcgc tgttcggtgt
    24901 catgttctgg gcaatttggt ccgatttgat caccgtgttc agctatctcg acagcatcac
    24961 gctctggcat tacaacggca ctgaagctgg cgctgcggtg gtgaaaaacg tcaccatggg
    25021 cagtctgttg tttgcgatta tcgcctcaat ggtggcctgg gcgttgattc gcaacctgcc
    25081 tggtttactg gaagtgctgg tgctctcgcg actgaatatg cgtcagggcg cgtcgtatgc
    25141 cattactacc atccttaact acatcattat tgccgttggt gcgatgacgg tgttcggatc
    25201 gctgggcgtc tcttgggata aactccagtg gctggccgca gcattatccg taggtcttgg
    25261 ttttggttta caagaaattt tcggtaactt cgtctccggt ttgatcattc tgttcgaacg
    25321 tccggtgcgt attggcgata cggtaaccat tggtagcttc tcggggacgg taagtaagat
    25381 ccgtattcgt gcgacaacga ttaccgattt cgatcgcaaa gaagtgatca tcccgaacaa
    25441 agcgtttgtt accgagcgtc tgatcaactg gtcgttgact gacactacta cgcgtctggt
    25501 gatccgtctc ggcgtggcct atggctccga tctggaaaaa gtgcgtaaag tgttactgaa
    25561 ggcggcgact gagcacccaa gggtgatgca cgaaccaatg ccggaagtct tctttacggc
    25621 gtttggtgcc agcacgttgg atcatgagct gcgtctgtat gtgcgtgaac tgcgtgaccg
    25681 tagtcgtact gtcgatgagc tgaaccgtac tatcgatcag ctgtgccgtg aaaacgacat
    25741 caacattgcc tttaaccagc ttgaagtgca tctgcacaac gagaagggcg atgaggtgac
    25801 ggaagtcaaa cgcgactaca aaggcgatga cccgacgcca gcggtagggt aataaccaaa
    25861 gggggcgaca tttagttgcc ccgagattac tgacaaagtg cgcgttgttc atgccggatg
    25921 cggcgtgacc gccttatccg gcctacgaaa ccgcaagaat tcaatgtatt gcaggagcgg
    25981 tgtaggcctg ataagcgtag cgcatcaggc agttttgcgt ttgcccacaa tcttagggga
    26041 catttagcgg ccccatttat ttctcacttt tccgccttat catcgcgcgt taatttcttt
    26101 tcataatcgc gttttacaat atccagcgcg cgcagcacgg tgcgggctgg gatctgattt
    26161 tcttccagca gcacaatcaa atcgacagcc agtttgacat cgtcaggggc attttcaagt
    26221 gacatactct ctccattgct agcgggttaa gcgcgctagc ctgttttcga ttttttccag
    26281 cgcatggcgg cagcgtgcca ggcgcgcttc ataggcttcc acttcacgat gcagcgtttg
    26341 ctgttccacg agatcggtca cccgcgccag gcgggctctg cgttcggcaa ccatctcacg
    26401 tagccgccgc tcaaaatcct gatgctgaat acgtttacgc tgccagcggg caattttcgg
    26461 tggcgcactg tcccactcgc gcaatgacca ggcggaggct tcacgcgcga tggcttccag
    26521 ttgtgccgcc agatgttccg ccagccaggc cacttgcggc agctgttgct gctcaaccgc
    26581 atgacaaagc gcagccagat tatcgcccgc ctcgtcgaga caagcctgta gtgttgtcgc
    26641 acgagtctga aaaagatgcc tgtcgaaacg agcacttagc gtggcgaact gtgacaccgg
    26701 ggcacaacgc tgacgcagcg tagcgagctg tccttccagt ttttccagca gcagggcggt
    26761 tttcacgata agacctcaat gaaaatgata attgttatgc taaagtagcc actctgtaag
    26821 ctgacaacac tcaatatgca acgaatcatt ttaatcatca ttggctggct ggcggtagtg
    26881 ctgggtacgc tgggcgtggt attaccggta ttaccgacga cgccgtttat cctgctggcg
    26941 gcctggtgct ttgcccgttc ttccccgcgc tttcacgcct ggttgctgta ccgctcatgg
    27001 tttggcagct atctacgttt ctggcagaaa catcatgcga tgccgcgcgg cgtcaaaccg
    27061 cgggcgattt tgcttatttt gctcacgttt gccatttctc tgtggttcgt ccagatgcca
    27121 tgggtgcgca tcatgttgct ggtaattctc gcctgtttgc ttttctatat gtggcgaatt
    27181 ccggtgattg atgaaaagca agaaaagcac tgaagcacaa caatcgcagt tgcaattatt
    27241 gcgtacagcc agtacattct ggcgttttcg agcacaggcg caggcggtca aaggttaaac
    27301 aactgttact tttgatacgt ttaaaacgcg ccgtgagtac caccgtaaca agcaggcata
    27361 cacttatgac cgcgactgca cagcagcttg agtatctcaa aaatagcatc aaaagcattc
    27421 aggactaccc aaaacccggc attcttttcc gcgatgtcac cagcttactg gaagacccga
    27481 aagcttacgc tctcagcatc gacttgctgg ttgagcgtta caaaaatgcg ggcattacca
    27541 aagttgtcgg caccgaagcg cgtggcttct tgtttggcgc tccggtagct ctgggtctgg
    27601 gcgttggctt tgtaccggtc cgtaaaccgg gcaaactgcc gcgtgaaacc atcagtgaaa
    27661 cttacgacct ggaatacggc accgatcagc tggagatcca cgttgatgcc atcaaaccgg
    27721 gcgacaaagt tctggtggtg gacgatctgc tggcaaccgg cggcactatc gaagcgaccg
    27781 ttaaactgat ccgtcgtctg ggtggtgaag tggctgacgc tgcgttcatt atcaacctgt
    27841 tcgatctcgg cggcgaacag cgtctcgaaa aacagggcat taccagctac agccttgtcc
    27901 cgttcccggg ccattaatta tcgccagtct tgtgctgccc acgctacgga cagcacaaga
    27961 tgtgcattca gcctcgccgt tctgacgggg ctgtgttagc attacccctt cgtgaatcca
    28021 ccttccagcg tttcagagcc tgccaatgag ttatcaggtc ttagcccgaa aatggcgccc
    28081 acaaaccttt gctgacgtcg tcggccagga acatgtgctg accgcactgg cgaacggctt
    28141 gtcgttaggg cgtattcatc atgcttatct tttttccggc acccgtggcg tcggaaaaac
    28201 ctctatcgcc cgactgctgg cgaaggggct aaactgcgaa accggcatta ccgcgacgcc
    28261 gtgcggcgtg tgcgataact gtcgtgaaat cgagcagggg cgctttgtcg atctgattga
    28321 aatcgacgcc gcctcgcgca ccaaagttga agatacccgc gacctgctgg ataacgtcca
    28381 gtacgctccg gcgcgtggtc gtttcaaagt ttatctgatc gacgaagtgc atatgctgtc
    28441 gcgccacagc tttaacgcac tgttaaaaac ccttgaagag ccgccggagc acgttaagtt
    28501 tctgctggcg acgaccgatc cacagaaatt gccggtgacg attttgtcgc gctgtttgca
    28561 atttcatctc aaggcgctgg atgtcgagca aattcgccat caacttgagc acatcctcaa
    28621 cgaagaacat atcgctcacg agccgcgggc gttgcaattg ctggcgcgcg ccgctgaagg
    28681 cagcctgcga gatgccttaa gtctgaccga ccaggcgatt gccagcggtg acggccaggt
    28741 ttcaacccag gcagtcagtg cgatgctggg tacgcttgat gacgatcagg cgctgtcgct
    28801 ggttgaagcg atggtcgagg ccaacggcga gcgcgtaatg gcgctgatta atgaagccgc
    28861 tgcccgtggt atcgagtggg aagcgttgct ggtggaaatg ctcggcctgt tgcatcgtat
    28921 tgcgatggta caactttcgc ctgctgcact tggcaacgac atggccgcca ttgagctgcg
    28981 gatgcgtgaa ctggcgcgca ccataccgcc gacggatatt cagctttact atcagacgct
    29041 gttgattggt cgcaaagaat taccgtatgc gccggaccgc cgcatgggcg ttgagatgac
    29101 gctgctgcgc gcgctggcgt tccatccgcg tatgccgctg cctgagccag aagtgccacg
    29161 ccagtccttt gcacccgtcg tgccaacggc agtaatgacg ccaacccagg tgccgccgca
    29221 atcagcgccg cagcaggcac cgactgtacc gctcccggaa accaccagcc aggtgctggc
    29281 ggcgcgccag cagttgcagt gcgtgcaggg agcaaccaaa gcaaaaaaga gtgaatcggc
    29341 agccgctacc cgcgcgcggc cggtgaataa cgctgcgctg gaaagactgg cttcggtcac
    29401 cgatcgcgtt caggcgcgtc cggtgccatc ggcgctggaa aaagcgtcag ccaaaaaaga
    29461 agcgtatcgc tggaaggcga ccactccggt gatgcagcaa aaagaagtgg tcgccacgcc
    29521 gaaggcgctg aaaaaagcgc tggaacatga aaaaacgccg gaactggcgg cgaagctagc
    29581 ggcagaagcc attgagcgcg acccgtgggc ggcacaggtg agccaacttt cgctaccaaa
    29641 actggtcgaa caggtggcgt taaatgcctg gaaagaggag agcgacaacg cagtatgtct
    29701 gcatttgcgc tcctctcagc ggcatttgaa caaccgcggt gcacagcaaa aactggctga
    29761 agcgttgagc atgttaaaag gttcaacggt tgaactgact atcgttgaag atgataatcc
    29821 cgcggtgcgt acgccgctgg agtggcgtca ggcgatatac gaagaaaaac ttgcgcaggc
    29881 gcgcgagtcc attattgcgg ataataatat tcagaccctg cgtcggtttt tcgatgcgga
    29941 gctggatgaa gaaagtatcc gccccatttg atcgtaagca cagcttacgt tcgtcatcct
    30001 taacgtgatt gagagagaaa cctatgtttg gtaaaggcgg tctgggtaac ctgatgaagc
    30061 aagcccagca gatgcaagaa aaaatgcaga aaatgcagga agagatcgcg cagctggaag
    30121 tcaccggcga atctggcgca ggtctggtaa aagtgaccat caacggtgca cacaactgcc
    30181 gtcgcgtaga gatcgacccg agcctgctgg aagacgacaa agagatgctg gaagacctgg
    30241 tggctgcagc attcaacgac gcagcacgtc gtattgaaga aacgcagaaa gaaaaaatgg
    30301 cctctgtatc ctccggaatg cagctgccgc ctggctttaa gatgccgttc tgatgcaaac
    30361 cagcccgctg ttaacacagc ttatggaagc actgcgctgt ctgccgggcg ttggcccgaa
    30421 gtcggcgcag cgtatggcgt tcacgctgct tcagcgcgat cgtagcggcg ggatgcgttt
    30481 ggcgcaggcg ctcacccggg cgatgtcgga aatcggccac tgcgccgatt gccgcacttt
    30541 caccgaacag gaagtctgta acatctgttc gaatccgcgt cgtcaggaaa acggtcaaat
    30601 ctgtgtggtg gagagtccgg cggacatcta cgccattgag cagacggggc agttttctgg
    30661 tcgttatttt gtgttgatgg ggcatctgtc accgctggac ggcatcggtc cggatgatat
    30721 cgggcttgat cgtctggaac agcgtctggc agaggaaaaa atcactgaag tgatcctcgc
    30781 caccaatccc acggttgaag gtgaagctac cgctaactac attgccgagc tttgcgcgca
    30841 atatgacgtg gaagccagcc gaatcgctca tggcgtaccg gttggcggcg aactggaaat
    30901 ggtcgatggc accacgctgt cacactccct tgccgggcgt cataagattc gtttttaagc
    30961 aaacgagagc aggatcacct gctctcgctt gaaattattc tcccttgtcc ccatctctcc
    31021 ctcatcctgt ttttaacctt aaaatggcat tattgaggta gacctacatg aaaggacaag
    31081 aaactcgtgg ttttcagtca gaagtgaaac agcttctgca cctgatgatc cattctctct
    31141 attccaataa agaaatcttc ctgcgtgagc ttatctctaa cgcctccgat gcggcggaca
    31201 agctgcgttt ccgtgcgcta tctaacccgg acctgtacga aggtgatggc gaactgcgcg
    31261 ttcgtgtctc tttcgataaa gacaagcgca cgctgaccat ctctgataac ggcgtgggga
    31321 tgacccgcga cgaagtgatt gaccatctgg ggactatcgc taaatccggt accaaatcat
    31381 tcctcgaatc cctgggttct gaccaggcga aagacagcca gctgatcggt cagtttggtg
    31441 ttggtttcta ctctgcgttt atcgtggccg acaaagtgac cgtgcgtact cgcgcggcag
    31501 gcgaaaaacc agaaaacggc gtcttctggg aatcggctgg cgaaggtgaa tataccgttg
    31561 ccgacatcac caaagaagat cgtggtactg aaatcaccct gcatctgcgt gaaggcgaag
    31621 acgagttcct cgatgactgg cgcgtgcgtt ccatcatcag caaatactcc gaccatatcg
    31681 cgctgccggt agagatcgaa aaacgcgaag agaaagacgg cgaaaccgtt atctcctggg
    31741 agaaaatcaa caaagcgcag gcgctgtgga ctcgtaacaa gtcggaaatc accgatgaag
    31801 agtacaaaga gttctacaaa cacatcgccc acgactttaa tgatccgctg acctggagcc
    31861 acaaccgcgt tgaaggtaag caggagtaca ccagcctgct gtacattccg tcccaggctc
    31921 cgtgggatat gtggaaccgt gatcataaac acggcctgaa actgtacgtc cagcgtgtgt
    31981 tcatcatgga cgacgcagaa cagttcatgc cgaactatct gcgcttcgtg cgtggtctga
    32041 ttgactccag cgatctgccg ctgaacgttt cccgtgaaat cctccaggac agcacggtaa
    32101 cgcgtaacct gcgcaatgcg ctgaccaagc gtgtgctgca aatgctggaa aaactggcga
    32161 aagacgacgc ggaaaaatac cagaccttct ggcaacagtt tggcctggta ctgaaagaag
    32221 gtccggcgga agatttcgct aaccaggaag cgatcgccaa actgctgcgt tttgcttcta
    32281 cccataccga ttcttctgcg cagaccgtat ctctggaaga ctacgtttcc cgcatgaaag
    32341 aagggcagga gaaaatctac tacatcaccg cagacagcta tgcggcagcg aagagcagcc
    32401 cgcacctgga actgctgcgt aagaaaggca tcgaagttct gctgctttcc gaccgcatcg
    32461 atgagtggat gatgaactat ctgactgagt tcgacggtaa accgttccag tcggtgtcta
    32521 aagttgacga gtcgctggaa aaactggctg acgaagttga tgagagcgcg aaagaagcgg
    32581 agaaagcact gactccgttc atcgaccgtg tgaaagccct gctcggcgag cgcgtgaaag
    32641 atgtccgtct gactcaccgt ctgaccgata cgccagcgat cgtttcgacc gacgcggacg
    32701 aaatgagcac tcagatggcg aaactgttcg ctgcggcggg ccagaaagtg ccagaagtga
    32761 aatacatctt cgaactgaac ccggatcacg tactggtgaa acgtgcggca gatactgaag
    32821 atgaagcgaa gttcagcgag tgggtagaac tgctgctgga tcaggcgctg ctggcagaac
    32881 gcggcacgct ggaagatccg aacctgttta ttcgtcgtat gaaccagctg ctggtttcct
    32941 gatgtcatgc cggatgacct ttgtgtcatc cggcattttt cttttcatca tctgcacttt
    33001 ccgcaaatta tctcgccatt aaccgtttca gcctcaggtg cctttcttga ggcaatcgcc
    33061 tgttggtggt atcgtttatc gctttttcaa aaaaatcgac acattttaag gggattttcg
    33121 caatgcgtat cattctgctt ggcgctccgg gcgcggggaa agggactcag gctcagttca
    33181 tcatggagaa atatggtatt ccgcaaatct ccactggcga tatgctgcgt gctgcggtca
    33241 aatctggctc cgagctgggt aaacaagcaa aagacattat ggatgctggc aaactggtca
    33301 ccgacgaact ggtgatcgcg ctggttaaag agcgcattgc tcaggaagac tgccgtaatg
    33361 gtttcctgtt ggacggcttc ccgcgtacca ttccgcaggc agacgcgatg aaagaagcgg
    33421 gcatcaatgt tgattacgtt ctggaattcg acgtaccgga cgaactgatt gttgatcgta
    33481 tcgtaggccg ccgcgttcat gcgccgtctg gtcgtgttta tcacgttaaa ttcaatccgc
    33541 cgaaagtaga aggcaaagac gacgttaccg gtgaagaact gactacccgt aaagacgatc
    33601 aggaagaaac cgtgcgtaaa cgtctggttg aataccatca gatgactgca ccgctgatcg
    33661 gctactactt caaagaagcg gaagcgggta acaccaaata cgcgaaagtt gacggcacca
    33721 agtcggttgc tgaagttcgc gctgatctgg aaaaaatcct cggctaatca agccgcagca
    33781 gatctgcttt cgggcaggtc tgctcatcca cctcaggcaa tacctctcac agcaattagt
    33841 tcttcttcct cacttttccg ctacaattat caacaagttg aatcgataag aggcggtaat
    33901 gcgtcagact aaaatcggta tcctgctggc aaacctgggt acgcccgatg cccccacacc
    33961 tgaagcggta aaacgctatc tgaaacaatt tttaagcgac agacgcgtgg ttgatacctc
    34021 acggttgtta tggtggccat tgctgcgcgg cgttattttg ccgctgcgct cgccgcgtgt
    34081 ggcgaagctg tacgcctcgg tctggatgga aggtggctcg ccgctgatgg tttacagccg
    34141 ccagcaacag caggcgctgg cacaacgttt accggagacg cccgtagcgc tgggaatgag
    34201 ctacggctcg ccatcactgg aaagcgccgt cgatgaactc ctggcagagc atgtagatca
    34261 tattgtggtg ctgccgcttt atccgcaatt ctcctgttct acggtcggtg cggtatggga
    34321 tgaactggca cgcattctgg cgcgcaaacg tagcattccg gggatatcgt ttattcgtga
    34381 ttacgccgat aaccacgatt acattaatgc actggcgaac agcgtacgcg cttcttttgc
    34441 caaacatggc gaaccggatc tgctgctgct ctcttatcat ggcattcccc agcgttatgc
    34501 agatgaaggc gatgattacc cgcaacgttg ccgcacaacg actcgcgaac tggcttccgc
    34561 actggggatg gcaccggaaa aagtgatgat gacctttcag tcgcgctttg gccgggaacc
    34621 ctggctgatg ccttataccg acgaaacgct gaaaatgctc ggagaaaaag gcgtaggtca
    34681 tatacaggtg atgtgcccgg gctttgctgc ggattgtctg gagacgctgg aagagattgc
    34741 cgagcaaaac cgtgaggtct tcctcggtgc gggcgggaaa aaatatgaat atattccagc
    34801 gcttaatgcc acgccggaac atatcgaaat gatggctaat cttgttgccg cgtatcgcta
    34861 aagctgagcg gtaaagaact gagcgccgtc gcgaagagcc tcgtcggcgg ttttcatcat
    34921 ccgtgaataa tgcaaaaagg catgcagcgt acctgggtag agtttgaact cacagggctg
    34981 ctgatgcgcc gctaacgtct ggtaaagcag acggctgtca tccagcagcg gatcgaactc
    35041 cgccccggca ataaaacagg gcggaacttc gcgagtgaga tcattattaa acagacagta
    35101 atacggtgac tcgcggtccg cgtcgttgct taaatacgcc tcttcataca tctgcaaatc
    35161 ctgttgcgtt aagccatccc agacaccgcc caacagacga cgagtcacgg aatcccgtaa
    35221 tccgtaaagc ccataccaca gcaaaacacc cgcaatttta ccgcaatcga tctgtttatc
    35281 acgcaaccac aacgcactgg cgagcgccag catggcacct gcggaatcac cggcaaagcc
    35341 aatgcgggac atattgattt gataatcctc cgcctgctgg tggaaataac aacaagcagc
    35401 cacaatttcc tctatcgctt gcggaaaacg cgcttcaggt gaaagggtgt aatcaatacc
    35461 aatcaccgta cattggctgt agcttgccag caggcgcatg atgcgatcgt gggtatcgag
    35521 attgccgaga ataaaaccgc ctccatgcaa ataaaatagc gtcgccgggc tatctggctg
    35581 cggacaaaag agacgtgttt ccacctgccc atattttgtt ggaaccatgt aagctctggt
    35641 tgccatttct ggagcgcccg cattccagaa tcggcgctca agcgtgtaat actgtcgttg
    35701 ctcagcaatc gttcccgttg cgggccagga cggtaaatcc ggctgaagag tattcacaac
    35761 ggtcttcatt tcagcagaaa taaggtccag aacaggtagt ttgttttccg gcttcataca
    35821 aaaactcctt tcaaattacg tcattgtaag gaaccactgc catgaaaatg cgatcccgcc
    35881 tgctgatatt gaaactggct gcgtctcgcg cgctcccgtc agattgtgtt aacattcgcc
    35941 gctcagttaa ccacccgtaa aaacaaccat gaaatttccc ggtaaacgta aatccaaaca
    36001 ttacttccct gtaaatgcac gcgatccgct gcttcagcaa ttccagccag aaaacgaaac
    36061 cagcgccgcc tgggtcgtag gtattgacca gacgctggtc gatattgaag cgaaagtgga
    36121 tgatgaattc attgagcgtt atggattaag cgccgggcat tcactggtga ttgaggatga
    36181 cgtagccgaa gcgctttatc aggagctaaa acagaaaaac ctgattaccc atcagtttgc
    36241 gggtggcacc attggtaaca ccatgcacaa ctactcggtg ctcgcggacg accgttcggt
    36301 gctgctgggc gtcatgtgca gcaatattga aattggcagt tatgcctatc gttacctgtg
    36361 taacacttcc agccgtaccg atcttaacta tctacaaggc gtggatggcc cgattggtcg
    36421 ttgctttacg ctgattggcg agtccgggga acgtaccttt gctatcagtc caggccacat
    36481 gaaccagctg cgggctgaaa gcattccgga agatgtgatt gccggagcct cggcactggt
    36541 tctcacctct tatctggtgc gttgcaaacc gggtgaaccc atgccggaag caaccatgaa
    36601 agccattgag tacgcgaaga aatataacgt accggtggtg ctgacgctgg gcaccaagtt
    36661 tgtcattgcc gagaatccgc agtggtggca gcaattcctc aaagatcacg tctctatcct
    36721 tgcgatgaac gaagatgaag ccgaagcgtt gaccggagaa agcgatccgt tgttggcatc
    36781 tgacaaggcg ctggactggg tagatctggt gctgtgcacc gccgggccaa tcggcttgta
    36841 tatggcgggc tttaccgaag acgaagcgaa acgtaaaacc cagcatccgc tgctgccggg
    36901 cgctatagcg gaattcaacc agtatgagtt tagccgcgcc atgcgccaca aggattgcca
    36961 gaatccgctg cgtgtctatt cgcacattgc gccgtacatg ggcgggccgg aaaaaatcat
    37021 gaacactaat ggagcggggg atggcgcatt ggcagcgttg ctgcatgaca ttaccgccaa
    37081 cagctaccat cgtagcaacg taccaaactc cagcaaacat aaattcacct ggttaactta
    37141 ttcatcgtta gcgcaggtgt gtaaatatgc taaccgtgtg agctatcagg tactgaacca
    37201 gcattcacct cgtttaacgc gcggcttgcc ggagcgtgaa gacagcctgg aagagtctta
    37261 ctgggatcgt taagttatcg tcggttcgta ggccagataa ggcgttcacg ccgcatctgg
    37321 catttggctc tcgatgcctg atgcgacgct ggcgcgtctt atcatgccta catatttttc
    37381 atattttaca tccggcaacc accgtttacc ccgtcaccac ttcacccgcc ggtggcgttt
    37441 ccagcagttc cagcatagta cgggcaattt cacgttcacc catcactacc tgattcgcgc
    37501 cacgttcggt gatatacgtc acttcatcgt cataatgggc gcgggcgata atctcaatat
    37561 ccgggttttt cgcgcgggca gacgccacaa tctcacccgc ttcataaccg ttggggatcg
    37621 tcaggatcag ccattttgca cattccagat gcgccagttg cataatttct tcgttcgctg
    37681 cattgcccaa tacagcgcgg accccacgct ctcgcagctc atcaacacgg gttcgtgacg
    37741 tctcaatcac caccagcgga atatcagagg cgagcaattt ctcccccagc aggctgccta
    37801 cacgaccgta acccaccaat agcgcatggt tgcaaatatc cactgggatc tgtttctctt
    37861 cttcgattgc ctcttccagc gtctgctctt ccagcgtttc ggtcttcgcc agatatttct
    37921 ccagtagtgc gaacagtacc gggttgagca taatcgacag gatcgcccct gccagtacca
    37981 ggttttgtcc ggcctgcggc agtaaattca atgccattcc cagtcccgcc aggataaacg
    38041 cgaactcacc aatctgcgcc aggctggcgg cgatggttaa tgccgtacgt tgggagtgac
    38101 caaacagacg gacaaggaac agggccgcga ccgatttacc aaacagaata attgccagcg
    38161 tcgccagcac tgccagcggt tgctgaatca gaattaacgg atcaaacaac atcccgacgg
    38221 agacaaaaaa cagcaccgca aacgcgtcgc gcaatggcag cgtatcgtgg gcggcacggt
    38281 gactcagttc agactcgttc agtaccatcc cggcaaagaa cgcgccaagt gcgaaggaga
    38341 cgtcaaacaa ctccaccgca ccaaaggcaa tccctaacgc cagcgccagc accgacaggg
    38401 taaatagctc gcgagaaccg gttgccgcgc tgcgtgccat aatccacggc accagacggc
    38461 gacctaccag catcataatg gcgataaatg cgatcacttt gccgatggtg atccccatat
    38521 cgactgcaag agtggcaaag cccacatcgc cctgttccat cattcctgcc actgcgggca
    38581 gcaacaccag cgtcagaacc attaccaggt cttccacaat caaccaaccg atggcgattt
    38641 gcccacgctg actgtcaatt aattgccgtt cttcaagtgc gcgcagtaac accacggtac
    38701 tggcggtgga aagacataaa ccgaacacga taccggtcat taacgaccag cccagcacgg
    38761 cagagagcgc catacccagc agcgtcgcca cggctatctg ggcgatcgca ccgggaatgg
    38821 cgatggcctt taccgccatc aaatccttca gcgaaaagtg caaaccgacg ccaaacatca
    38881 acagaatgac gcccagttca gccagttccg gcgcaagctt ggtatcggca acaaagcccg
    38941 gagtgaatgg tcctgccagc acacccgcta acagatatcc caccagagga gaaatacgta
    39001 gtttattggc cagcatgccg aggataaagg cgagcacaag gccgccaaca atggtggtga
    39061 taagcggggt ggcgtgatgc attccgtctc cttttcctgg tggttattgt ccatttttgg
    39121 ccgggaaaac caaaattaca ggtaatagtt tatgacaatt tcattgatga tgttcatgaa
    39181 taattattga attttgcaga aaaatggaat tagctgcaaa aaaagcacgg atcggaaaac
    39241 agaaggcggt ttaacagagg aaaggcttat ggcgactgta aaacgatgca gccaaagttt
    39301 gcctttggct gcaatgaaat cagtctttat gccggttatc aggcaggaat atggtcaaca
    39361 tccccaatag tggcaggaaa gcacagattt tatagactaa ctcgatg
//
LOCUS       DAJTZL010000016        39401 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.016, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000016 DAJTZL010000000
VERSION     DAJTZL010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39401
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.016"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(159..371)
                     /gene="rpmE"
                     /locus_tag="OO544_000736"
     CDS             complement(159..371)
                     /gene="rpmE"
                     /locus_tag="OO544_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HCR6766412.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            574..2772
                     /gene="priA"
                     /locus_tag="OO544_000737"
     CDS             574..2772
                     /gene="priA"
                     /locus_tag="OO544_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HCR6766413.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMAVKAGCRVRVPFGKQQERIG
                     VVVSVSDVSELPLNELKAVVEVLDVEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQTLRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPDVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIINGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            2928..3953
                     /gene="cytR"
                     /locus_tag="OO544_000738"
     CDS             2928..3953
                     /gene="cytR"
                     /locus_tag="OO544_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HCR6766414.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            4045..5004
                     /gene="ftsN"
                     /locus_tag="OO544_000739"
     CDS             4045..5004
                     /gene="ftsN"
                     /locus_tag="OO544_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HCR6766415.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            5097..5627
                     /gene="hslV"
                     /locus_tag="OO544_000740"
     CDS             5097..5627
                     /gene="hslV"
                     /locus_tag="OO544_000740"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HCR6766416.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            5637..6968
                     /gene="hslU"
                     /locus_tag="OO544_000741"
     CDS             5637..6968
                     /gene="hslU"
                     /locus_tag="OO544_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HCR6766417.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            7035..7961
                     /gene="menA"
                     /locus_tag="OO544_000742"
     CDS             7035..7961
                     /gene="menA"
                     /locus_tag="OO544_000742"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HCR6766418.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLVLITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            8054..8539
                     /gene="rraA"
                     /locus_tag="OO544_000743"
     CDS             8054..8539
                     /gene="rraA"
                     /locus_tag="OO544_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HCR6766419.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(8624..8869)
                     /gene="zapB"
                     /locus_tag="OO544_000744"
     CDS             complement(8624..8869)
                     /gene="zapB"
                     /locus_tag="OO544_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HCR6766420.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            9295..10140
                     /gene="glpF"
                     /locus_tag="OO544_000745"
     CDS             9295..10140
                     /gene="glpF"
                     /locus_tag="OO544_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HCR6766421.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            10163..11674
                     /gene="glpK"
                     /locus_tag="OO544_000746"
     CDS             10163..11674
                     /gene="glpK"
                     /locus_tag="OO544_000746"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HCR6766422.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDVM"
     gene            11804..12814
                     /gene="glpX"
                     /locus_tag="OO544_000747"
     CDS             11804..12814
                     /gene="glpX"
                     /locus_tag="OO544_000747"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HCR6766423.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            12911..13657
                     /gene="fpr"
                     /locus_tag="OO544_000748"
     CDS             12911..13657
                     /gene="fpr"
                     /locus_tag="OO544_000748"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HCR6766424.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLEE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(13662..14090)
                     /gene="uspD"
                     /locus_tag="OO544_000749"
     CDS             complement(13662..14090)
                     /gene="uspD"
                     /locus_tag="OO544_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HCR6766425.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(14117..14416)
                     /locus_tag="OO544_000750"
     CDS             complement(14117..14416)
                     /locus_tag="OO544_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HCR6766426.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(14628..15068)
                     /locus_tag="OO544_000751"
     CDS             complement(14628..15068)
                     /locus_tag="OO544_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCR6766427.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFVPTLILVMMLIDLGAFVGTQGENKYGKDTQDVKYKADNKSSN"
     gene            15169..15768
                     /locus_tag="OO544_000752"
     CDS             15169..15768
                     /locus_tag="OO544_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HCR6766428.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNSEKGLTFAVEPIKLALSESLEGLNK"
     gene            15876..16643
                     /gene="tpiA"
                     /locus_tag="OO544_000753"
     CDS             15876..16643
                     /gene="tpiA"
                     /locus_tag="OO544_000753"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HCR6766429.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(16698..17453)
                     /gene="cdh"
                     /locus_tag="OO544_000754"
     CDS             complement(16698..17453)
                     /gene="cdh"
                     /locus_tag="OO544_000754"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HCR6766430.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHQCEILR"
     gene            complement(17560..18549)
                     /gene="sbp"
                     /locus_tag="OO544_000755"
     CDS             complement(17560..18549)
                     /gene="sbp"
                     /locus_tag="OO544_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HCR6766431.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(18869..19831)
                     /gene="pfkA"
                     /locus_tag="OO544_000756"
     CDS             complement(18869..19831)
                     /gene="pfkA"
                     /locus_tag="OO544_000756"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HCR6766432.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(20012..20914)
                     /gene="fieF"
                     /locus_tag="OO544_000757"
     CDS             complement(20012..20914)
                     /gene="fieF"
                     /locus_tag="OO544_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HCR6766433.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(21062..21562)
                     /gene="cpxP"
                     /locus_tag="OO544_000758"
     CDS             complement(21062..21562)
                     /gene="cpxP"
                     /locus_tag="OO544_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HCR6766434.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            21712..22410
                     /gene="cpxR"
                     /locus_tag="OO544_000759"
     CDS             21712..22410
                     /gene="cpxR"
                     /locus_tag="OO544_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HCR6766435.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            22407..23780
                     /gene="cpxA"
                     /locus_tag="OO544_000760"
     CDS             22407..23780
                     /gene="cpxA"
                     /locus_tag="OO544_000760"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HCR6766436.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(23886..24560)
                     /gene="yiiM"
                     /locus_tag="OO544_000761"
     CDS             complement(23886..24560)
                     /gene="yiiM"
                     /locus_tag="OO544_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein"
                     /protein_id="HCR6766437.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWVREFPEQAELFVAPAFGENLSTDGLTESNVHMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(24709..25692)
                     /gene="kdgT"
                     /locus_tag="OO544_000762"
     CDS             complement(24709..25692)
                     /gene="kdgT"
                     /locus_tag="OO544_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HCR6766438.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(25952..26572)
                     /gene="sodA"
                     /locus_tag="OO544_000763"
     CDS             complement(25952..26572)
                     /gene="sodA"
                     /locus_tag="OO544_000763"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HCR6766439.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            26857..27891
                     /gene="rhaT"
                     /locus_tag="OO544_000764"
     CDS             26857..27891
                     /gene="rhaT"
                     /locus_tag="OO544_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HCR6766440.1"
                     /translation="MSNAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGIV
                     SWIILPWTISALLLPDFWAYYSSFSLSTLLPVFLFGAMWGIGNINYGLTMRYLGMSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLINTEGGRMTLLGVLVALIGAGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAMNAAKPMHEAAAALGVDPLYVALPSYV
                     VIMGGGAIINLGFCFIRLAKVKDLSLKADFSLAKPLIIHNVLLSALGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWNNTGRRPVTVLSLGCVVIIVA
                     ANIVGIGMAN"
     gene            complement(27888..28736)
                     /gene="rhaR"
                     /locus_tag="OO544_000765"
                     /pseudo
     CDS             complement(27888..28736)
                     /gene="rhaR"
                     /locus_tag="OO544_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
     gene            complement(28810..29646)
                     /gene="rhaS"
                     /locus_tag="OO544_000766"
     CDS             complement(28810..29646)
                     /gene="rhaS"
                     /locus_tag="OO544_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HCR6766441.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRAHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            29934..31403
                     /gene="rhaB"
                     /locus_tag="OO544_000767"
     CDS             29934..31403
                     /gene="rhaB"
                     /locus_tag="OO544_000767"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HCR6766442.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWNVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRSNGLMAQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPEAMCSEIQAACREMAQPLPESDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQLHSTRQTKELCA"
     gene            31400..32659
                     /gene="rhaA"
                     /locus_tag="OO544_000768"
     CDS             31400..32659
                     /gene="rhaA"
                     /locus_tag="OO544_000768"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HCR6766443.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQAIGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHPDDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLDP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKAILSQRG"
     gene            32901..33725
                     /gene="rhaD"
                     /locus_tag="OO544_000769"
     CDS             32901..33725
                     /gene="rhaD"
                     /locus_tag="OO544_000769"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HCR6766444.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAP
                     YHGNFHAQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     STLDETFGLIDTAEKSAQVLVKVYSMGGMKQTISREELIALGKRFGVTPLASALAL"
     gene            33735..34049
                     /gene="rhaM"
                     /locus_tag="OO544_000770"
     CDS             33735..34049
                     /gene="rhaM"
                     /locus_tag="OO544_000770"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HCR6766445.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFATVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            34350..34796
                     /locus_tag="OO544_000771"
     CDS             34350..34796
                     /locus_tag="OO544_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HCR6766446.1"
                     /translation="MAALTASCIDLNIQGNGAYSVLKQLATIALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQDFIHQLQQGDADQVLALLNQTLSS"
     gene            34807..36258
                     /locus_tag="OO544_000772"
     CDS             34807..36258
                     /locus_tag="OO544_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HCR6766447.1"
                     /translation="MESSLCIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISQALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            36248..37318
                     /locus_tag="OO544_000773"
     CDS             36248..37318
                     /locus_tag="OO544_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCR6766448.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVRHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GFKIPGVIGSVAPHALTEKQKQQPLSFDEMFIDIGANSRDEVEKRGVAMGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVAALKSCAAH
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RDFLTTLTAEKVNAFSQFRQVD"
     gene            37318..39066
                     /locus_tag="OO544_000774"
     CDS             37318..39066
                     /locus_tag="OO544_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative frv operon regulatory protein"
                     /protein_id="HCR6766449.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARIFASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPRLKQRYERTCCLASRPGLGHFIDETEEKRVILLANLLRKDPFLIPLA
                     GVTRDNFQHLSTACDNQHRWPLMQGDYLSSLILAIYALRNQLTDEWPQYPGNEIKQIV
                     EQSGMFLGDNAVRTLTGLIEKQHQQAQVISADHVLGLLQRVPGIASLNIIDTQLVENI
                     TGHLLRCLAAPVWIAEHRQSSMNNLKAAWPAAFDMSLHFITLLREQLDIPLFDSDLIG
                     LYFACALERHQNERQPIILLSDQNAIATINQLAIERDVLHCRVIIARSLSELIAIREE
                     IEPLLIINNSHYLLDDAVNNYITVKNIITAADIEQIKHFLATAFIRQQPERFFSAPGS
                     FHYSNVRGESWQHITRQICAQLVAQHHITADEAQRIIAREGEGENLIVNRLAIPHCWS
                     EQERRFRGFFITLAQPVEVNNEVINHVLIACAAADARHELKIFSYLASVLCQHPAEVI
                     AGLTGYEAFMELLHKG"
     gene            complement(39115..>39401)
                     /locus_tag="OO544_000775"
     CDS             complement(39115..>39401)
                     /locus_tag="OO544_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3829 domain-containing protein"
                     /protein_id="HCR6766450.1"
                     /translation="DVDAAMKKVSELETLVAQAKEADKGGMNFSFINSADQYQLETKK
                     YVRRVRDKVPYSDWDKEHLQDANTSWMVEDSFPRALREYNEMVDDYNSLR"
BASE COUNT         9587 a        10217 c        10223 g         9374 t
ORIGIN      
        1 gtaaaatcct tttgaaataa ataagaacca gaagcctagc gccagtcaac aacaggaaat
       61 gataatgagt taacaccccg gaatttttca gaatttataa aaggcaaatt acaggcacaa
      121 aaaaagcgcc gtgcggcgct tttttcggaa atccggtctt atttgctgcc cgggatgttg
      181 aaacgcttgt tgaagcggtc aacacggcca ccggtagcaa catcacgctg tttgccagtg
      241 aagaacgggt ggcacttgct gcacacgtcg aggttcaggt catgaccaac ggtggagcgg
      301 attttcatta cgttaccgca agagcagcta gcagtaattt cttcgtattt cgggtgaata
      361 tcttttttca tgggaaaacc tcggtttaag gccgcgtcgc tcttccagcc ctaacgccag
      421 acaccacgcg atgttaaaag tatagcttca atacgatcat ttcgtacgaa ggcgcgaaat
      481 catacagaaa ttaaccagcg tatgcaaact gatccgcact cttctacggc aatgtgtata
      541 ctaacccacc gaatttcaag tcaggatgat gctatgcccg ttgcccacgt tgccttgccc
      601 gttccgcttc ctcgtacctt tgactatctg ctgccagaag gcatggcggt taaagccggg
      661 tgtcgcgtgc gcgtgccgtt tggcaaacag caggagcgca tcggggttgt ggtatcagtt
      721 agcgatgtca gtgagctgcc gcttaacgag ctgaaagcgg tagttgaagt gctggacgtt
      781 gagccggtat ttactcactc cgtctggcga ttactgctct gggcggcaga ttactatcat
      841 catccgattg gcgatgtgct gtttcatgcc ttgccgattt tactgcgcca ggggcggcct
      901 gcggcgaacg cgccgatgtg gtactggttt gccactgaac aaggccaggc ggtggatctg
      961 aacagcctga aacgctcacc caagcaacaa caggcgctgg cggcgttacg gcaagggaaa
     1021 atctggcgcg accaggttgc cacgctcgaa tttaatgatg ccgcgttgca gacgctgcgc
     1081 aaaaaaggtc tgtgtgattt agcaagtgaa acaccagagt ttagcgactg gcgaacgaac
     1141 tatgccgttt ctggtgagcg gttgcgattg aataccgaac aggccaccgc cgttggcgca
     1201 attcatagcg cggcagatac tttttctgcc tggctgctgg cgggcgttac cggttccggt
     1261 aaaacggagg tttatctcag tgtactggaa aacgtgctcg ctcagggcaa acaggcgctg
     1321 gtgatggtgc cggaaatcgg cctgacaccg caaactatcg cccgttttcg tgaacggttt
     1381 aatgcgcccg tggaagttct gcattccggt ctgaacgaca gcgagcgtct ttcggcgtgg
     1441 ctgaaagcga aaaatggcga ggcggcgatt gtgatcggca cccgctccgc gctgtttacg
     1501 ccgtttaaaa atctcggcgt gattgtcatt gacgaagagc acgacagttc ctacaagcag
     1561 caggaaggct ggcgctatca tgcccgcgac ctggcggtgt atcgtgcgca tagcgagcaa
     1621 atcccgatta ttcttggctc cgcaacgccc gcgctggaaa cgttgtgcaa cgtccagcag
     1681 aaaaaatacc gcctgctgcg cctgacgcgc cgggcgggga atgcgcgtcc ggcaattcaa
     1741 cacgtgctgg atttaaaagg tcagaaggtg caggcaggtc tggctccggc gttaatcacc
     1801 cgtatgcgcc agcatttaca ggccaacaat caggttatcc tctttcttaa ccgccgtggc
     1861 tttgcgcctg cgctactgtg ccacgactgc ggctggattg ccgaatgccc acgttgcgat
     1921 cactactaca cgctgcatca ggcgcaacag catttgcgct gtcaccactg tgacagtcag
     1981 cgtccggtgc cgcgccagtg cccttcctgc ggttccacgc acctggtacc cgtggggctg
     2041 ggcaccgaac agcttgaaca gacgcttgcg ccgctgttcc ctgatgtgcc catttctcgt
     2101 atcgaccgcg ataccaccag ccgcaaaggg gcgctggaac agcaactggc ggaagtacat
     2161 cgcggcggtg cgaggatttt gatcggcaca caaatgctgg cgaaaggtca ccactttccg
     2221 gatgtgacgc tggtggcgtt actggatgtg gacggcgcgc tgttttctgc cgattttcgc
     2281 tcggcagagc gtttcgctca gctttacacc caggtcgccg gtcgtgccgg gcgtgcgggt
     2341 aaacagggcg aagtggtgct gcaaacgcac catccggaac atcctctgtt gcaaacgttg
     2401 ctctataaag gctacgacgc ctttgccgaa caggcgctgg ctgagcgacg catgatgcag
     2461 ctgccgccgt ggaccagcca tgtgattgtg cgtgcggaag atcataacaa tcagcatgcg
     2521 ccgctgttcc tgcaacaact gcgtaatctg atcctctcca gcccgctggc agacgacaaa
     2581 ttgtgggttc tcggtccggt tccggcactg gcacctaaac gtggcggtcg ctggcgctgg
     2641 cagatattgt tgcagcaccc ttcccgcgtg cgcttgcaac acatcattaa cggtacgctg
     2701 gcactcatca atacaatacc ggattcccgt aaggtgaaat gggtgctgga tgttgatccg
     2761 attgagggtt aaaccgctca cgatgcgagg cggatcgaaa aattcaatat tcatcacact
     2821 tttcatgaaa attctgtaac cgttttcacg cgctatctgc taaaaatgtt gccgatgtga
     2881 agtaaacatg gatgtagtac gcctgacgtg ccaggcgagg agtgagtgtg aaagcgaaga
     2941 agcaggaaac tgccgcgacc atgaaagacg ttgccctcaa ggcaaaagtc tctacagcga
     3001 ctgtctcccg agcattaatg aatcccgata aagtctccca ggccacccgt aatcgggttg
     3061 aaaaagcggc ccgggaagtg ggttatttac cgcagcctat ggggcgcaac gtcaagcgta
     3121 atgaatcccg caccattctg gtgattgtcc cggatatctg cgatcccttc tttagcgaaa
     3181 ttattcgcgg tatcgaagtt acggcggcaa atcacggata tctggtgctg attggcgact
     3241 gtgcgcatca aaatcagcag gaaaaaacct ttatcgattt gatcatcacc aagcaaattg
     3301 atggcatgtt gctgctgggt tcaaggctgc cgtttgatgc cagcattgag gaacagcgta
     3361 atctgccgcc gatggtgatg gcgaacgaat tcgcaccgga actggagcta cctacagttc
     3421 atatcgacaa tctgaccgcc gcatttgatg cagtaaatta tctatatgag caagggcata
     3481 aacggattgg ctgtatagcc ggtcccgaag agatgccgct gtgtcactac cgcctgcaag
     3541 gttacgttca ggcgctgcgt cgctgcggca ttatggttga tccgcaatac atcgcccgtg
     3601 gcgacttcac cttcgaagcc ggaagcaaag cgatgcagca gctgcttgat cttccacaac
     3661 cgcctactgc tgtcttctgc catagcgatg tgatggcgct cggcgcactt tctcaggcaa
     3721 aacgccaggg gctgaaagtc ccggaagacc tttccataat cggttttgat aacatcgacc
     3781 tgacgcaatt ttgtgatccg ccgctgacaa ccatcgcgca gccgcgttac gaaatcggtc
     3841 gggaagctat gctgttattg cttgatcaaa tgcaggggca acacgttggc agtggctctc
     3901 gtttaatgga ctgcgaactt atcatccggg gatcaacacg cgcgttacct taaagtaaac
     3961 ctttaagact gccgtgactg gtcaaaggcc cgccgcttaa gtaacatggc gggctgacga
     4021 acgaataaat acagcgaaac gatagtggca caacgagatt atgtacgccg cagccaaccg
     4081 gcaccttcgc ggcgaaaaaa gagcacctca cggaaaaagc aacgaaatct gcctgcggtt
     4141 tctcccgcta tggtcgctat tgctgccgcc gtgcttgtga cctttatcgg tggtctgtac
     4201 ttcattacgc atcacaagaa agaagagtcc gagacgctgc aaagccagaa agtgaccggt
     4261 aacggactac caccaaaacc agaagaacgc tggcgctaca ttaaagagct ggaaagtcgc
     4321 cagccgggtg tgcgtgcgcc cacagaacct tctgccggtg gtgaagtgaa aacgccggag
     4381 caactgacac cagaacaacg tcagcttctt gaacaaatgc aggctgatat gcgccagcag
     4441 ccaacgcagc tggttgaagt tccgtggaat gaacagacgc cggaacagcg tcagcaaacg
     4501 ctacaacgcc agcgtcaggc gcagcaatta gcggagcagc aacgcctggc tcagcagtcg
     4561 cgtacgactg aacaaagctg gcagcagcag acgcgtacgt cgcaagccgc gccggtacag
     4621 gcgcaaccgc gccagtctaa accggcttct actcagcagc cgtaccagga tctgctgcaa
     4681 acccctgcgc acacgaccgc gcagtcgaag ccgcagcaag ctgcgccggt tgctcgtgcc
     4741 gctgacgcgc caaaaccgac ggcggagaaa aaagacgaac gccgctggat ggtgcagtgc
     4801 ggttcgttca gaggcgcgga acaggcagag acggtacgtg ctcagctggc gttcgaagga
     4861 tttgactcga aaatcaccac caacaatggc tggaatcgtg tggtcattgg cccggtgaaa
     4921 ggcaaagaga acgcagacag caccctcaat cggttgaaga tggcgggtca tacaaactgc
     4981 attcggctcg ccgccggggg ttgaaaccct caaaatcccc cccatctata attgcattat
     5041 gccccgtact tttgtacggg gtttgtactc tgtattcgta accaaggggt cagctcgtga
     5101 caactatagt aagcgtacgc cgtaacggcc atgtggtcat cgctggtgat ggtcaggcca
     5161 cgttgggcaa taccgtaatg aaaggcaacg tgaaaaaggt ccgccgtctg tacaacgaca
     5221 aagtcatcgc gggctttgcg ggcggtactg cggatgcttt tacgctgttc gaactgtttg
     5281 aacgtaaact ggaaatgcat cagggccatc tggtcaaagc cgccgttgag ctggcaaaag
     5341 actggcgtac cgatcgcatg ctgcgcaaac ttgaagcact gctggcagtc gcggatgaaa
     5401 ctgcatcgct tatcatcacc ggtaacggtg acgtggtgca gccagaaaac gatcttattg
     5461 ctatcggctc cggcggccct tacgcccagg ctgcggcgcg cgcgctgtta gaaaacactg
     5521 aacttagcgc ccgtgaaatt gctgaaaagg cgttggatat tgcaggcgac atttgcatct
     5581 ataccaacca tttccacacc atcgaagaat taagctacaa agcgtaagga tctcccatgt
     5641 ctgaaatgac cccacgcgaa atcgtcagcg aactggataa gcacatcatc ggccaggaca
     5701 acgccaagcg ttctgtggcg attgctctgc gtaaccgctg gcgtcgcatg cagctcaacg
     5761 aagagctgcg ccatgaagtg accccgaaaa atatcctgat gatcggcccg accggtgtcg
     5821 gtaaaactga aatcgcccgt cgtctggcta agctggcgaa tgcgccgttc atcaaagttg
     5881 aagcgaccaa attcaccgaa gtgggctatg tcggtaagga agtggattct attattcgcg
     5941 atctgaccga tgccgccgtg aaaatggtac gcgtccaggc tatcgagaaa aaccgttatc
     6001 gcgctgaaga actggcagaa gaacgtattc tcgacgtgct gatcccacct gctaaaaaca
     6061 actggggaca gaccgaacag cagcaggaac cgtccgctgc tcgtcaggca ttccgcaaaa
     6121 aactgcgtga aggccagctt gatgacaaag aaatcgagat cgatcttgcc gcagcaccga
     6181 tgggcgttga aattatggct cctccaggca tggaagagat gaccagccag ctgcagtcca
     6241 tgttccagaa cctgggcggc cagaagcaaa aagcgcgtaa gctgaaaatc aaagacgcca
     6301 tgaagctgct gattgaagaa gaagcggcga aactggtgaa cccggaagag ctgaagcaag
     6361 acgctatcga cgctgttgag cagcacggga tcgtgtttat cgacgaaatc gacaaaatct
     6421 gtaagcgcgg cgagtcttcc ggtccggatg tttctcgtga aggcgttcag cgtgacctgc
     6481 tgccgctggt agaaggttgc accgtttcca ccaaacacgg gatggtcaaa actgaccaca
     6541 ttctgtttat cgcttctggc gcgttccaga ttgcgaaacc gtctgacctg atcccggaac
     6601 tgcaaggtcg gctgccaatc cgcgttgaac tgcaggcact gaccaccagc gacttcgagc
     6661 gtattctgac cgagccgaat gcctctatca ccgtgcagta caaagcactg atggcgactg
     6721 aaggcgtaaa tatcgagttt accgactccg gtattaagcg catcgcggaa gcggcatggc
     6781 aggtgaacga atctaccgaa aacatcggtg ctcgtcgttt acacactgtt ctggagcgtt
     6841 taatggaaga gatttcctac gacgccagcg atttaagcgg tcaaaatatc actattgacg
     6901 cagattatgt gagcaaacat ctggatgcgt tggtggcaga tgaagatctg agccgtttta
     6961 tcctataatc gcgttcaatc attttcatca ttgtttgatg gggctgaaag gccccatttt
     7021 tattggcgcg tattatgact gaacaacaaa ttagccgaac tcaggcgtgg ctggaaagtt
     7081 tacgacctaa aaccctcccc ctcgcctttg ctgcaattat cgtcgggacg gcgctggcat
     7141 ggtggcaagg tcacttcgat ccgctggtcg ccctgctggt actaattacc gccgggctat
     7201 tacagatcct ttctaacctc gccaatgatt acggcgatgc ggtaaaaggc agcgataaac
     7261 ctgaccgcat tgggccgcta cgcggcatgc aaaaaggggt cattacccag caagagatga
     7321 aacgggcgct cattattacc gtcgtgctca tctgtctctc cgggctggca ctggttgcag
     7381 tggcatgcca tacgctggcc gattttgtcg gtttcctgat tcttggcggg ttgtcgatca
     7441 ttgccgctat cacctacacc gtgggcaatc gtccttatgg ttatatcggt ctgggtgata
     7501 tttccgtact ggttttcttt ggctggttga gcgtcatggg gagctggtat ttacaggctc
     7561 atacattgat tccggcactg atccttccgg cgaccgcctg tggcctgctg gcaacggcag
     7621 tactgaacat taataacctg cgtgatatca atagcgaccg cgaaaatggc aaaaacacgc
     7681 tggtggtgcg cttaggtgaa gtgaacgcgc gtcgttatca tgcctgcctg ctgatgggct
     7741 ctctggtatg tctggcgctg tttaatctct tttcgctgca tagcctgtgg ggctggctgt
     7801 tcctgctggc ggcaccatta ctggtgaagc aagcccgtta tgtgatgcgg gaaatggacc
     7861 cggtggcgat gcgaccaatg ctggaacgta ctgtcaaggg agcgttactg actaacctgc
     7921 tgtttgtttt agggatattc ctaagccagt gggcagcata actgacaaat atcaattaac
     7981 aattgatgat tttgccaaca gcctacatag cgcgatatac tgaaaattct cgcagcaact
     8041 gaatgttaag cctatgaaat acgatacttc cgagctttgt gacatctatc aagaagatgt
     8101 taacgtcgtg gaaccgctgt tctccaactt tggcggacgg gcgtcgtttg gcggacaaat
     8161 aatcacggta aaatgtttcg aggacaacgg gttgctgtac gatctgctcg aacagaatgg
     8221 ccgtggtcgt gttcttgtcg tcgatggcgg tggttctgtt cgtcgcgcac tggtcgatgc
     8281 tgaactggcg cgtctggcag tacaaaatga atgggaaggt ctggttattt acggcgcggt
     8341 gcgtcaggta gatgacctag aagagttgga tatcggcatc caggcgatgg cggcaattcc
     8401 ggttggtgcc gctggcgaag gcattggcga aagcgatgtc cgcgtcaatt ttggcggtgt
     8461 aaccttcttc tccggcgacc atctttatgc cgacaatacc gggattattc tttcagaaga
     8521 tccgctggat attgaatgat aagaaaggca ccgcaaggtg ccttttttct gcgttacctg
     8581 ttggcctaca cagtaaagaa attacgcgaa agatgaagcg taatcagacc tcttccatgc
     8641 gacccagcag ggcctgcaga cgttcctgcc agccgttctg ctgttctttc agatggttgt
     8701 tctcacgctc cagctcttcg cgctgatgct gggcattttg aacttcctgc gacagtgagt
     8761 tgtttttttc tttcagctct tcgatttcca tctgcaacag agtgatggta tcaatcgcct
     8821 gctgtacttt tgcttccagt ttctcaaaca cttctaatga cattgtcata cctctcctga
     8881 attgcaaggc gttgatggat aaaaaatcct cgtcccgatt accggtgacg ccttaataaa
     8941 tacgagcgca ctttagttag ctccgattgt atgaagccgc gccatcgctg tccagcggca
     9001 cgccttgcag attacggttt gccacacttt tcatccttct cctggtgaca taatccacat
     9061 caatcgaaaa tgttaataaa tttgttgcgc gaatgatcta acaaacatgc atcatgtaca
     9121 atcagatgga ataaattgcg cgataacgct cattttatga cgaagcacac acattttaag
     9181 ttcgatattt ctcgtttttg ctcgttaacg ataagtttac agcatgccta caagcatcgt
     9241 ggaggtccgt gactttcacg catacaacaa acattaactc ttcaggatcc gattatgagt
     9301 caaacatcaa ccttgaaagg ccagtgcatt gctgaattcc tcggtaccgg gttgttgatt
     9361 tttttcggtg tgggttgcgt tgcagcacta aaagtcgctg gtgcgtcttt tggtcagtgg
     9421 gaaatcagtg tcatttgggg actgggggtg gcaatggcca tctacctgac cgcaggggtt
     9481 tccggcgcgc atcttaatcc cgctgttacc attgcattgt ggctgtttgc ctgtttcgac
     9541 aagcgcaaag ttattccttt tatcgtttca caagttgccg gcgctttctg tgctgcggct
     9601 ttagtttacg ggctttacta caatttattt ttcgacttcg agcagactca tcacattgtt
     9661 cgcggcagtg ttgaaagtgt tgatctggca ggcactttct ctacttaccc taatcctcat
     9721 atcaattttg tgcaggcttt cgcagttgag atggtgatta ccgctattct gatggggctg
     9781 atcctggcgt taacggacga tggcaacggt gtaccacgcg gccctttggc tcccttgctg
     9841 attggtctac tgattgcggt cattggcgca tctatgggcc cattgacggg ttttgccatg
     9901 aacccagcgc gtgacttcgg tccgaaagtc tttgcctggc tggcgggctg gggcaatgtc
     9961 gcctttaccg gcggcagaga cattccttac ttcctggtgc cgctttttgg ccctatcgtt
    10021 ggcgcgattg taggtgcatt tgcctaccgc aaactgattg gtcgccattt gccttgcgat
    10081 atctgtgttg tggaagaaaa ggagaccaca actccttcag aacaaaaagc ttcgctgtaa
    10141 tatgactacg ggacaattaa acatgactga aaaaaaatat atcgttgcgc tcgaccaggg
    10201 caccaccagc tcccgcgcgg tcgtaatgga tcacgatgcc aatatcatta gcgtgtcgca
    10261 gcgcgaattt gagcaaatct acccaaaacc aggctgggta gaacacgacc caatggaaat
    10321 ctgggccacc caaagctcca cgctggtaga agtgctggcg aaagccgata tcagttccga
    10381 tcaaattgca gctatcggta ttacgaacca gcgtgaaacc actattgtct gggaaaaaga
    10441 aaccggcaag cctatctata acgccattgt ctggcagtgc cgtcgtaccg cagaaatctg
    10501 cgagcattta aaacgtgacg gtttagaaga ttatatccgc agcaataccg gtctggtgat
    10561 tgacccgtac ttttctggca ccaaagtgaa gtggatcctc gaccatgtgg aaggctctcg
    10621 cgagcgtgcg cgtcgtggtg aattgctgtt tggtacggtt gatacgtggc ttatctggaa
    10681 aatgactcag ggccgtgtcc atgtgaccga ttacaccaac gcctctcgta ccatgttgtt
    10741 caacatccat accctggact gggacgacaa aatgctggaa gtgctggata ttccgcgcga
    10801 gatgctgcca gaagtgcgtc gttcttccga agtgtacggt cagactaaca ttggcggcaa
    10861 aggcggcacg cgtattccaa tctccgggat cgccggtgac cagcaggccg cgctgtttgg
    10921 tcagttgtgc gtgaaagaag ggatggcgaa gaacacctat ggcactggct gctttatgct
    10981 gatgaacacc ggcgagaaag cggtgaaatc agaaaacggc ctgctgacca ccatcgcctg
    11041 cggcccgact ggcgaagtga actatgcgct ggaaggtgcg gtgtttatgg caggcgcatc
    11101 cattcagtgg ctgcgcgatg agatgaagtt gattaacgac gcctacgatt ccgaatattt
    11161 cgccaccaaa gtgcaaaaca ccaatggtgt gtatgtggtt ccggccttta ccgggctggg
    11221 tgcgccgtac tgggacccgt atgcgcgcgg ggcgattttc ggtctgactc gtggggtgaa
    11281 cgctaaccac attatacgcg cgacgctgga gtctattgct tatcagacgc gtgacgtgct
    11341 ggaagcgatg caggccgact ctggtatccg tctgcacgcc ctgcgcgtgg atggtggcgc
    11401 agtagcaaac aatttcctga tgcagttcca gtccgatatt ctcggcaccc gcgttgagcg
    11461 cccggaagtt cgcgaagtga ctgcattggg cgcggcctat cttgctggtc tggcggtcgg
    11521 tttctggcag aacctcgacg agctgcaaga gaaagcggtg attgagcgcg agttccgtcc
    11581 aggcatcgaa accactgagc gtaattaccg ttacgcaggc tggaaaaaag cggttaaacg
    11641 cgcgatggcg tgggaagaac acgacgtaat gtaaatgccg gatgaagcgt ttatgccgca
    11701 tccggtagtc ccgaaacgtg cgggggcaac cccgcacaca tcaataatcc ctcccttccc
    11761 ctgtgctaca cttcgcgcca ttccttactg cttagagttt gctatgagac gagaacttgc
    11821 catcgaattt tcccgcgtca ccgaatctgc ggcgctggct ggctacaaat ggttaggacg
    11881 cggcgataaa aacaccgcgg acggcgcggc ggtaaacgcc atgcgtatta tgctcaacca
    11941 ggtcaatatt gacggcacca tcgtcattgg tgaaggtgaa atcgacgaag caccgatgct
    12001 ctacattggt gaaaaagtcg gtactggtcg cggcgacgcg gtagatattg ctgttgatcc
    12061 gattgaaggc acgcgcatga cggcgatggg ccaggctaac gcgctggcgg tgctggcagt
    12121 aggcgataaa ggctgcttcc tcaatgcgcc ggatatgtat atggagaagc tgatcgtcgg
    12181 accgggagcc aaaggcacca ttgatttgaa cctgccgctg gcggataacc tgcgcaatgt
    12241 tgcggcggcg ctcggcaaac cgttgagcga actgacggta acaattctgg ctaaaccacg
    12301 ccacgatgcc gttatcgctg aaatgcaaca actcggcgta cgcgtatttg ctattccgga
    12361 tggcgacgtt gcggcctcaa ttctcacctg tatgccagac agcgaagttg acgtgctgta
    12421 cggtattggt ggcgcgccgg aaggcgtagt ttctgcggcg gtgatccgcg cattagatgg
    12481 cgacatgaac ggtcgtctgc tggcgcgtca tgacgtcaaa ggcgacaacg aagagaatcg
    12541 tcgcattggc gagcaggagc tggcacgctg caaagcgatg ggcatcgaag ccggtaaagt
    12601 attgcgcctg ggcgatatgg cgcgcagcga taacgtcatc ttctctgcca ccggtattac
    12661 caaaggcgat ctgctggaag gcattagccg caaaggcaat atcgcgacta ccgaaacgct
    12721 gctgatccgc ggcaagtcac gcaccattcg ccgcattcag tctatccact atctggatcg
    12781 caaggacccg gaaatgcagg tgcacatcct ctgattgatt tgatcgattg agccttccag
    12841 tccttcggga ctggaatttt tttgttcgga gaacgaagat aaggcaagtc aatcaaaaca
    12901 ggagaaaaac atggctgatt gggtaacagg caaagtcact aaagtgcaga actggaccga
    12961 cgccctgttt agtctcaccg ttcacgcccc cgtgcttccg tttaccgccg ggcaattcac
    13021 caagcttggc cttgaaatcg acggcgaacg cgtccagcgc gcctactcct atgttaactc
    13081 gcccgataat cccgatctgg agttttacct ggtcaccgtc cccgatggca aattaagccc
    13141 acgactggcg gcactgaaac caggcgatga agtgcaggtg gttagcgaag cagctggctt
    13201 ctttgttctg gaggaagtac cagattgcga aacgctatgg atgctggcaa ccggtacagc
    13261 gattggccct tatttatcga ttctgcaact aggcaaagat ttagatcgct tcaaaaatct
    13321 ggtcctggtt cacgccgcac gttatgccgc cgacttaagc tatttgccac tgatgcaaga
    13381 actggaaaaa cgctacgaag gcaaactgcg cattcagacg gtggtcagtc gggaaacggc
    13441 agcggggtcg ctcaccggac ggataccggc attaattgaa agtggggaac tggaaagcgc
    13501 gattggcctg ccgatgaata aagaaaccag ccatgtgatg ctgtgcggca atccacagat
    13561 ggtgcgcgat acacaacagt tgctgaaaga gacccggcag atgacgaaac atttacgtcg
    13621 ccgaccgggc catatgacag cggagcatta ctggtaagcg gttacttatc gataaacggc
    13681 acgatgagca aatccgcact catcttattg atcatcccgc gatatgccgg catcaaacgg
    13741 ttgataaatg agtggtgatg accacagaca aggaggtcgc actgctcttt ttgcataatt
    13801 tccagcagtg tttccggcat ttctccgcgt tcaatacgca gttttgtctt cggccattga
    13861 atatttttcg tcagtttata cagcttgtta tccgacttat tcttcaacaa ttgaagaata
    13921 tcttctgttg cagggaagta gatacccgga tacaactcgc ttaagccatc atcaatatga
    13981 attaacgtca ggtgagcgtc attatgtctg gcgagctcca gggctttatt caccagtaag
    14041 gcatcttctt cattcccgga aattgccacg ccaatgtgtt tataagccat gtttaactcc
    14101 ttctaaagca actccatcaa gctaacgaac gcagtgataa ctcaaaaata atcatctcgg
    14161 cctggcatga gaaagtgaag gccgcatcaa gctcaacttg cccctccaga tccttaatct
    14221 tataggccac gctggcccct tcatctgcag aggatgcagc ggcggcaagc tctttcagtt
    14281 tggtcataaa agactctgct tcttcgcggg tggcaaaaaa gcgcgaaaac ttactggtac
    14341 agttgtcgtt atcaatcacc gtaccgatat caatcgcaca tcctttagta ctgcatttat
    14401 ctacgacatc tttcataggg cacctctgta tattcgcctc tctgtcgatt acgacttaag
    14461 aataatgcgc cgttaaggga agaacaaaca agtaatgatc ttatggatta ccattttttt
    14521 tgatattgct taaggtgaag agttcagaag gattttccgg gtattgtcat ttgcatcgtg
    14581 gaaataacaa tcccggaaaa aaatagcagt aagagatata ttaaaggtta attacttgat
    14641 ttattgtcgg ctttatactt cacatcctga gtatctttac catatttatt ttcgccttgt
    14701 gtgccaacaa acgcaccaag atcgataagc atcatcacca gaatcaacgt cgggacaaaa
    14761 cgccccaccg cccattgcca gacacccggt aaaatcgccc agttacccgc cagcagcatc
    14821 cacgccacaa tcatcagaaa tgcccatgcg ccggaacgcc cgcgatcatg caagcgctta
    14881 acagttactg ccgctgttgg ccaaagcaag cacacaaggc aaaacgccgc ggtctgaata
    14941 tcgagtaaat ttttacccgc cagtgagaaa agcaccagca tgcctgcgaa ccacaggcct
    15001 atccaaatcc agaaatcacg gcgtccaata cgccctttaa atgagaataa ccattgctgt
    15061 atggtcatgt aagttccttg atggttgtct tttccaggat tctacccttt tgacaagggc
    15121 gacagatatc gttttaatcg gagccagtca taacaaaagg tactgtcaat gaagccaggg
    15181 tgtacgctgt tttttctctt atgttctgca ttaaccgtta caacaacggc gcatgcacaa
    15241 acaccagata cggcaacgac cgcgccttat ctgctggctg gagcccctac tttcgatctc
    15301 tccatcagcc agtttcgaga agactttaac agccagaatc ctagcctgcc actgaacgaa
    15361 tttcgtgcca tcgacagcag tcccgacaaa gccaatctca ctcgtgctgc aagtaaaatt
    15421 aacgagaact tgtatgcttc tacagcgctg gagcgcggta ccttaaaaat caaaagcatt
    15481 caaatgacct ggctacccat ccaggggcca gagcaaaaag cagcgaaagc gaaagctcag
    15541 gaatacatgg cagcagtgat ccgcacactc accccattaa tgaccaaaac acaaagccag
    15601 aaaaaactgc agtcgctact aacggcgggg aaaaacaaac gttattacac cgagacagaa
    15661 ggtgcactgc gttatgttgt cgcggacaac agcgaaaagg ggctgacctt cgctgttgaa
    15721 ccgattaagc tggcgctatc tgaatcgctt gaaggtttga ataaatgaca aaaagcaaag
    15781 cctttgtgcc gatgaatctc tatactgttt cacagacctg ctgccctgcg gggcggccat
    15841 cttcctttat tcgcttataa gcgtggagaa ttaaaatgcg acatccttta gtgatgggta
    15901 actggaaact gaacggcagc cgccacatgg ttcacgagct ggtttctaac ctgcgtaaag
    15961 agctggcagg tgttgctggc tgtgcggttg caatcgcacc accggaaatg tacatcgata
    16021 tggcgaagcg cgaagctgaa ggcagccaca tcatgctggg tgcgcaaaac gtggacctga
    16081 acctgtccgg cgcattcacc ggtgaaactt ctgccgctat gctgaaagac atcggcgctc
    16141 agtacatcat catcggccac tctgaacgtc gtacttacca caaagagtct gacgaactga
    16201 tcgcgaaaaa attcgcggtg ctgaaagagc agggcctgac tccggttctg tgcatcggtg
    16261 aaaccgaagc tgaaaacgaa gcgggcaaaa ctgaagaagt ttgcgcacgt cagatcgacg
    16321 cggtactgaa aactcagggt gctgcggcat tcgaaggtgc ggttatcgct tacgaacctg
    16381 tatgggcaat cggtactggc aaatctgcaa ctccggctca ggcacaggct gttcacaaat
    16441 tcatccgtga ccacatcgct aaagttgacg ctaacatcgc tgaacaagtg atcattcagt
    16501 acggcggctc tgtaaacgcg tctaacgctg cagaactgtt tgctcagcca gatatcgacg
    16561 gcgcgctggt tggcggtgct tctctgaaag ctgacgcttt cgcagtgatc gttaaagctg
    16621 cagaagcggc taaacaggct taagtctgac aggtgccgga tttcatatcc ggcacttact
    16681 ttccttaact cttcgcctta acgcaaaatc tcacactgat gatcctgaat ttcctcggct
    16741 gaagcacggt taagcgtcag tagatttcgt tgtgtcgcca gcaatacaaa tgagttatca
    16801 ctctgccgta ccatcgccag cccgtagctt cccatatgtt cccgcgcctc aggtacttct
    16861 tctgccagca tcataaatgg gctgcgttgt accagttcgc tttccgttac ccgacgcgcc
    16921 aggtattcat gcccacgcaa accacctggc agtggcaacc agcggctgct gatgttcgcc
    16981 agattgttat ccagttgttc gcgcacatca ggacgaatac aagagatatg aatatgaaaa
    17041 tggttttgcg tacgcccggt gcgggagttg atcgccaaag aaaccgcgcg atcgggaacc
    17101 ggctggccgt attttttgct cataaaatca cgcgcctgcc aggccaacca aaagaagttc
    17161 ggcgttgaag gatcggtcaa caaaggactt tcagtaccgt taatacgata cgttggcatc
    17221 aacagatatt gcagtggacc attaagatct tttaaaacca cgtatccggc attgggtttg
    17281 acttccgcac atggcgaagg attttgattt tgctgctgat tgggcaaaca ttcctcaagg
    17341 acaatcttac gtaatgtatc cgactcttca ccggttaatt tccagtaacc aataccggca
    17401 gccacaacgg cgataactat catcaccaaa aaaagaagac ccgctttttt catctttttt
    17461 tccctgtacc tcaaagagat gcaagggtaa cgcaaaatcg tgacaaataa aaaacccggt
    17521 cgcgagaaac caccgggttt gataattatc ctggggaaat cagcgtttgc tgatctgatc
    17581 gaacgtaccg ccgttagcaa aatgctcttt ttgcgctttc gtccagccgc cgaactcttc
    17641 atcaatggtg aataacttca gctttggaaa cgcattttcg tactttttcg ctacttcagc
    17701 gtcgcgcgga cggtagtagt ttttcgcggc aatttcctga ccttctggcg agtagagata
    17761 tttcaggtag gcttccgcca cctctttggt gccttttttc tcgaccactt tatcgaccac
    17821 cgacacggtt ggctctgcga ggatagactc actcggcgtg acgatttcga atttatcttt
    17881 ccccagttca ttcgctgcca gcagagcttc gttttcccag gcgatcagta catcgccaat
    17941 cccgcgctcg acaaaggtgt tagtggagcc gcgcgcgcca gaatccagaa cttcgacgtt
    18001 tttatacagt gcccgaacaa aatcctgtgc ttttgcctga tcgttgttgt tgtgatgcag
    18061 cgcgtagccc caggcagcta ggtagttcca gcgtgcgcca ccagagcttt tcggattagg
    18121 cgtgatcacc gaaacacccg gtttaatcag atcgttccag tcatggatct gcttcggatt
    18181 gcccttacgc accaggaaaa caatggtgga agtgtacggt gcggagttat ccggcagacg
    18241 tttgatccac tctttatcaa tccgcccacg ttccgcaatc gcgtccacgt cataggccag
    18301 agccagcgtg acaacatcag cttcaatacc gttgattacc gacgtcgctt gtttacctga
    18361 accaccgtgt gactgacgaa tcaccacgtt atcaccagtt tgctgtttcc agtgggcgct
    18421 gaatgccttg ttgtactgtt cgtacaattc gcgcgttgga tcatatgaaa cgttaagaag
    18481 ctgaatatcc tttgccataa cgctggttgc cgccagcaaa aatgttaacc ctacgcccca
    18541 cttgttcatc gcccgactct cttatgttgt gttgtgatga gcaaagcgtg ccagaaggtt
    18601 aaccaaacat taaagaataa aaaaagattg gctataactt gcgggtatat gttgagggat
    18661 taaaaaggcg gaagaaatcc gcctcatatt gctgacaaag tgcgctttgt ccatgccgga
    18721 tgcggcgcga acgccttatc cggcctacaa aagtttgcaa attcaataaa ttgcagaatt
    18781 catgtaggcc tgataagcga agcgcatcag gcatttttgc ttctgtcatc ggtttcaggc
    18841 taaaggaatc tgcctttttc cgaaatcatt aatacagttt tttcgcgcag tccagccagt
    18901 cacctttgaa cggacgcttc atgttttcga tagcgtcgat gatgtcgtgg tgaaccagct
    18961 gttcgttctg gatacctaca caacgaccgc cgtaacctgc cagcagcaga tcgatagcgt
    19021 aagcgcccat acgggaagcc agaatacggt cgtaaggcac cggagaacca ccgcgctgga
    19081 tgtggcccag cacagttgcg cgggtttcac gaccggtttc tttctcgatg aaatgcgcca
    19141 gttcgtcaac atcacacata tgttcggtaa tcgccacgat cgcgtgtttt ttacctttcg
    19201 cgatacccgc tttgatttcg tttaccaggt cttcacggct gaattcaact tccggaacca
    19261 caacgaattc acagccaccg gcaatggccg cagccaacgt cagatcgcca caataacggc
    19321 ccatcacttc caccacggaa atacgctggt gagaagaaga ggtgtcacgc agacggtcga
    19381 tcgcttctac aacggtgctc agcgcagtga agaaaccgat agtgtagtca gtgcctttga
    19441 tgtcgttgtc gatagtgccc ggcagaccga tgcacgggaa gcccatttcg gtcagacgca
    19501 ttgcacccat gtaggaaccg tcaccgccga taaccaccag cgcgtcgata ccacgttttt
    19561 tcaggttttc gatagccacg gcgcggatgt tctcgtcgcg gaattccggg aagcgcgcag
    19621 aaccgaggaa cgtaccgcca cggttgatca tgtcagaaac gctgtaacgg tctagctgta
    19681 ccatacggtc ttcatacaga cccagatagc cgtcataaat gcccattact tccagacctt
    19741 ctgtcagcgc agaacgaaca accccgcgaa ttgcggcgtt catgcctggc gcatcaccgc
    19801 cgcttgtcaa cacaccgatt ttcttaatca tgactacctc tgaactttgg aatgcaaaat
    19861 aaaatctgtt gccggaagtc ttcttgcaca tcgaagtgat ccaacgaatg tgcaaatagt
    19921 ataacaatca cttcctgctg aattgattca ggtcaggcca aatggcggta ttttatacac
    19981 aaaatgcggg tctggctctc ttttatactg attatgaaag catagaccgt ttaccctccc
    20041 tgggtacgac ggaacagggg tcctgatgga taattacatc cgatcccgga aaacgccgta
    20101 aaatagcctg ctctacctga tccgccacca tatgtgcctg aaccaaaggc agagagtctt
    20161 ccatttccaa atgaatctga ataaagcggg tcggccctga ctgccgcgtg cgaagatcgt
    20221 gagcgccgct aacacccggc caggaagtca cgatatcaat aatttcttgc cgttcctcat
    20281 caggcaatgc gcgatccagt aatgactgta ccgcctcata tcccatgcgt aacgcgctat
    20341 ataaaatata gatgccgatt cccaatgcaa acagagcatc ggcgcgatgc cagccgtacc
    20401 aggacaaccc cagcgccagc agaattgcgc cgttcatcat aacatcagac tggtaatgta
    20461 gcatatcagc ccgcaccgcc tggctttgcg tccggcgcac cacccaccgc tgaaacgaga
    20521 caaggataat cgtacaaatt agcgccacaa ttgtcacgat aaccccgacg cctggatctg
    20581 tcatcggtgt tggagatacc agatgttgaa tacccgtcaa aaacaggaat agtgccgaac
    20641 cggagataaa catactttgc gccagcgccg cgagggactc agctttaccg tgaccaaacg
    20701 agtgattatc gtcggcaggt tgcagggaat atcgcaccac cagtaaattc gtcaacgacg
    20761 cgccgatatc caccagcgaa tccaccagcg cggcgagaat actcaccgac ccggtatacc
    20821 accatgcaaa aattttaatc agcaatagca gcgaagccat cgccgtcgca gcaatcgccg
    20881 cccgactgac cagccgtcca taagattgat tcataaatac tcccactatc aactgacgct
    20941 agtataacgg aagcaaatca tctgcaatgc attaagcagc aggcaaattg aggataaaaa
    21001 aaaccccaca tcatgtgggg gaagacaggg atggtgtcta tggcaaggaa aacagggttt
    21061 actactggga acgtgagttg ctactactca atagcttcaa cgatgaactt ttttgccatt
    21121 gcgtcacgtc acgcaactgc tccattcgtt gttgatgttt ctcgtttaaa accgcttgct
    21181 gctccggcgt taacaggcga tacatttggt tgcggacttt tgccatctca acctgacgag
    21241 caatttgctc attcgccatt ttttctgcct gtgcgcgcac agcgttttca tcaaaatttt
    21301 ctgcggtgac aaggcgatgc attgtctcca gttcgctaac attaacagga ggctgttcgt
    21361 gccgggcctg ttgcataaga tctcgcatct gctgacgctg atgttcggtt aaacttatgc
    21421 cgtcgaacat atggctctgc gtactgcgct gcgtaagttc ttcacccgga tgccagttat
    21481 cgcctgaacc gacttcagca gcgtggctta atgaactgac tgccagcgtt gaggccatga
    21541 cggcagcggt aactatgcgc atcatttgct cccaaaatct ttctgtcgcg attcaacgat
    21601 agagagttta cgattcaggc tgcaaacatg cgtcaggggg tgtaaaacaa cgtaaagtca
    21661 tggattagcg acgtctgatg acgtaatttc tgcctcggag gtatttaaac aatgaataaa
    21721 atcctgttag ttgatgatga ccgagagctg acttccctat taaaggagct gctcgagatg
    21781 gaaggcttca acgtgattgt tgcccacgat ggggaacagg cgcttgatct tctggacgac
    21841 agcattgatt tacttttgct tgacgtaatg atgccgaaga aaaatggtat cgacacatta
    21901 aaagcacttc gccagacaca ccagacgcct gtcattatgt tgacggcgcg cggcagtgaa
    21961 cttgatcgcg ttctcggcct tgagctgggc gcagatgact atctcccgaa accgtttaat
    22021 gatcgtgagc tggtggcacg tattcgcgcg atcctgcgcc gttcgcactg gagcgagcaa
    22081 cagcaaaaca acgacaacgg ttcaccgaca ctggaagttg atgccttagt gctgaatcca
    22141 ggccgtcagg aagccagctt cgacgggcaa acgctggagt taaccggtac tgagtttacc
    22201 ctgctctatt tgctggcaca gcatctgggt caggtggttt cccgtgaaca tttaagccag
    22261 gaagtgttgg gcaaacgcct gacgcctttc gaccgcgcta ttgatatgca catttccaac
    22321 ctgcgtcgta aactgccgga tcgtaaagat ggtcacccgt ggtttaaaac cttgcgtggt
    22381 cgcggctatc tgatggtttc tgcttcatga taggcagctt aaccgcgcgc atcttcgcca
    22441 tcttctggct gacgctggcg ctggtgttga tgttggtttt gatgttaccc aagctcgatt
    22501 cacgccagat gaccgagctt ctggatagcg aacagcgtca ggggctgatg attgagcagc
    22561 atgtcgaagc ggagctggcg aacgatccgc ccaacgattt aatgtggtgg cggcgtctgt
    22621 tccgggcgat tgataagtgg gcaccgccag gacagcgttt gttattggtg accaccgaag
    22681 gccgcgtgat cggcgctgaa cgcagcgaaa tgcagatcat tcgtaacttt attggtcagg
    22741 ccgataacgc cgatcatccg cagaagaaaa agtatggccg cgtggaactg gtcggtccgt
    22801 tctccgtgcg tgatggcgaa gataattacc aactttatct gattcgtccg gccagcagtt
    22861 ctcaatccga tttcattaac ttactgtttg accgcccgct attactgctg attgtcacca
    22921 tgttggtcag tacgccgctg ctgttgtggt tggcctggag tctggcaaaa ccggcgcgta
    22981 agctgaaaaa cgctgccgat gaagttgccc agggaaactt acgccagcac ccggaactgg
    23041 aagcggggcc acaggaattc cttgccgcag gtgccagttt taaccagatg gtcaccgcgc
    23101 tggagcgcat gatgacctct cagcagcgtc tgctttctga tatctctcac gagctgcgca
    23161 ccccgctgac gcgtctgcaa ctgggtacgg cgttactgcg ccgtcgtagc ggtgaaagca
    23221 aggaactcga gcgtattgaa accgaagcgc aacgtctgga cagcatgatc aacgatctgt
    23281 tggtgatgtc acgtaatcag caaaaaaacg cgctggttag cgaaaccatc aaagccaacc
    23341 agttgtggag tgaagtgctg gataacgcgg cgttcgaagc cgagcaaatg ggcaagtcgt
    23401 tgacagttaa cttcccgcct gggccgtggc cgctgtacgg caatccgaac gccctggaaa
    23461 gtgcgctgga aaacattgtt cgtaatgctc tgcgttattc ccatacgaag attgaagtgg
    23521 gctttgcggt agataaagac ggtatcacca ttacggtgga cgacgatggt cctggcgtta
    23581 gcccggaaga tcgcgaacag attttccgtc cgttctatcg gaccgatgaa gcacgcgatc
    23641 gtgaatctgg cggtacagga ttggggctgg cgattgttga aaccgccatt cagcagcatc
    23701 gtggctgggt gaaggcagaa gacagcccgc tgggcggttt acggctggtg atttggttgc
    23761 cgctgtataa gcggagttaa actccgcatt tgtaggcagg ataaggcgtt tacgccgcat
    23821 ccggcatttg agcaggatgc ctgatgcgac gctgatagcg tcttatcagg cctacactcc
    23881 gggggttatt ttccccacaa ccggcgggaa aaatcttcga tcttgccgct cagtcggcgc
    23941 ttttgcatcg tccgcgtcca gcttttcgat aacccggcgg cggagagtaa acggtgatac
    24001 tgatcgtcat caaacggcat atgccatgcg atggcggcag cctcctgcac ggtcacatcg
    24061 ctgacacggg aaaccaactc cagcggcgcg tccgcagaca ctttccctgg cgctatcacg
    24121 ctatacaacc accccacctt acctgtgttt tgcatcaact gcgctatatc gctgatatca
    24181 aaatggtaat tgagtttata gcaaggcgag cgcggctggc tgacctgaat taatgcctct
    24241 ccccagcgga aaatatcgcc catatgaaca ttactttccg tgagaccgtc ggttgagaga
    24301 ttttcgccaa acgcaggcgc aacaaacaac tccgcctgtt ccggaaattc ccgcacccag
    24361 tagagatagt gctcacgagg ataatgacac agcgctctgt ctggcccgcc gtgaaccttt
    24421 ttctccgcct gctcgtcacc ttccagcccc agctccgtca gcatcaactc accatcaacc
    24481 tggattttgg caattgcgct gggtttgcct tcgggataag cctgaatctt gcctgtgtat
    24541 acatcaaccg gatatcgcat ctgttccctc tccacgagaa atttccccat tagaccatat
    24601 ttattttatg tcaggcgtgc tgttttcgcg gtaagtatcc aggcaggaac tcaatagcaa
    24661 accggacgaa cgttagttca tccggctgtg gggattaagg aagcaggttt atttcaccgt
    24721 acctaaaatt tcgattttcg ctgctctggc tttgacttta cgtgaccaga tagaggtaag
    24781 aattggcacc agaatcgaag tcacaatgac cgccgtcgct accagcgaag ttgctgccgg
    24841 agccatcggt ttaaacgcag gcaccatttc tgcaatcagc acaggtgtcg ctaccgcggc
    24901 ccctgcggaa ctggaagcgg caattccggc agtgccatcg ccaccgccaa tcaatttatc
    24961 ggcgataatc aacggaatac cggtcacgat aattactgcc acacccaaca ggatccccag
    25021 caaaccagtc tgggcaatca cagtcaaatc aatggtattg cccagcgcga aggcaaagaa
    25081 tggaatcagc gtttgcactg ctttgctgaa aaattctcgt aattcagggt caagattccc
    25141 cagggcaaag cccaccagga acggtaatac tgcgccgacg aaaacatgcg gttcaaacga
    25201 ggcaatcccg gcagtgccca gaataatcat cgtcatgagc ggcccggact ccaacgacat
    25261 caacacaaat gccccagctt cttctttcgt accgtactgc tgcatgatgg aagcgtaaag
    25321 tccgccgttg gtcatatcca tcgccgccac cagcgccagc gttgaaagtc cggcaaagaa
    25381 tccaacttca acaccatgtt ccggaataat gcgcgaggca attgccgcaa ccacccacgc
    25441 gacggcaatt ttagttacca ccagcgtacc ggatttacgc agtaccgttc ccgtcgcgct
    25501 taattttatt gacgccccca tgcaaaaaaa ccacaccgcc agaatgggca ccgtaccggt
    25561 aatcatcccg ttggtgaatg atccaaaata tttccccgcc cccggcgaga aggtgtggca
    25621 cagtgcgcca aggaatagcg ggacgagcat catccccccc gggattttct caatcgagcg
    25681 ttttatctgc atttccatca cctgtcggca tatttttaag ggctgatgcg taattcctca
    25741 atggagcatc agtgttgatg gaaacgaaga tagacattca tttaatcaat aaaagtgatc
    25801 aaaaccacat caattgaaac gctgttttat ttttatcgga tcattgtttt tattttgaaa
    25861 ttgatcacaa aaaaaccacc gccgttggcg atggttcatt ttttaagctg atatgcggcc
    25921 tatacgcctc attgcagcag gcggcaaatg attatttttt cgccgcaaaa cgtgccgctg
    25981 cttcgtccca gttcaccacg ttccagaact ctttaatgta gtccggacgg cggttctgga
    26041 atttcaggta gtaagcatgt tcccacacat ccaggcccag aatcgggaag ccggaagcgc
    26101 cagaaatagc ttcacccatc agcggagaat cctggttagc agtagaaacc accgccagtt
    26161 tatcgccttt cagcaccagc catgcccagc cggaaccaaa gcgggaagct gccgcttttt
    26221 caaattctgc tttgaagtta tcaacggagc cgaagtcacg ttcgatagcc gctttcaggt
    26281 caccctgcag ggtggtgcct tttttcagac ctttccagaa caggctgtgg ttagcgtgac
    26341 cgccagcgtt gttgcgcagt acggttttct tgtctgctgg cagctggtcc agtttggtga
    26401 tcagctcttc aaccggcagg ttggcaaatt ctggcaggct ttccagcgcc gcgttggcgt
    26461 tgtttacgta ggtctgatgg tgtttggtgt ggtggatttc catggtctgc ttatcgaagt
    26521 gcggttccag ggcatcgtaa gcatacggca gggatggcag ggtatagctc atattcatct
    26581 ccagtattgt cgggcggccg attgttaatg ccgcgtaagc agttggttca ttatagttaa
    26641 ttaaatgata ttgaaaatga ttatcaatgc cgtacttttc gtaagggtat ggttttgcag
    26701 gaaaatgccc gagatgtgaa gcaaatcacc cacttaatgc cgtgattgcc agtaaatcga
    26761 caacggcggc aacaggcgaa aggttaatcg acagcacgat ttttacactc atctcgtcgg
    26821 agatgtgaag cgacgaaaaa tgatgaggat aagaagatga gtaacgcgat tacgatgggg
    26881 atattttggc atttgatcgg cgcggccagt gcagcctgtt tttacgctcc gttcaaaaaa
    26941 gtaaaaaaat ggtcatggga aaccatgtgg tcagtcggtg ggattgtttc gtggattatt
    27001 ctgccgtgga ccatcagcgc cctgttacta ccggatttct gggcgtatta cagctcgttt
    27061 agtctctcta cgctgctgcc tgtttttctg ttcggcgcta tgtgggggat cggtaatatc
    27121 aactatggcc tgaccatgcg ttatctcggc atgtcgatgg gaattggcat cgccattggc
    27181 attacgttga ttgtcggtac gctgatgacg ccaattatca acggcaattt cgatgtgctg
    27241 attaacaccg aaggcggacg catgacgttg ctcggcgttc tggtggcgct gattggcgca
    27301 gggattgtaa ctcgagccgg gcagttgaaa gagcgcaaga tgggcattaa agccgaagag
    27361 ttcaatctga aaaaagggct ggtgctggcg gtgatgtgcg gcattttctc tgccgggatg
    27421 tcctttgcga tgaacgccgc aaaaccgatg catgaagccg ctgccgcact tggcgtcgat
    27481 ccactgtatg tcgctctgcc aagctatgtt gtcatcatgg gcggcggcgc gatcattaac
    27541 ctcggtttct gttttattcg tctggcaaaa gtgaaggatt tgtcgctaaa agccgacttc
    27601 tcgctggcaa aaccgctgat cattcacaat gtgttactct cggcactggg cgggttgatg
    27661 tggtatctgc aattcttttt ctatgcctgg ggccacgccc gcattccggc gcagtatgac
    27721 tacatcagtt ggatgctgca tatgagtttc tatgtattgt gcggcggtat cgtcgggctg
    27781 gtgctgaaag agtggaacaa tacaggacgc cgtccggtaa cggtgttgag cctcggttgt
    27841 gtggtgatta ttgtcgccgc taacatcgtc ggcatcggca tggcgaatta atctttctgc
    27901 gaattgagat gacgccactg gctgggcgtc atcccggttt cccgggtaaa caccaccgaa
    27961 aaatagttac tatcttcaaa gccacattcg gtcgaaatat cactgattaa caggcggcta
    28021 tgctggagaa gatattgcgc atgacacact ctgacctgtc gcagatattg attgatggtc
    28081 attccagtct gctggcgaaa ttgctgacgc aaaacgcgct cactgcacga tgcctcatca
    28141 caaaatttat ccagcgcaaa gggacttttc aggctagccg ccaaccgggt aatcagctta
    28201 tccagcaacg tttcgctgga tgttggcggc aacgaatcac tggtgtaacg atggcgattc
    28261 agcaacatca ccaactgccc gaacagcaac tcagccattt cgttagcaaa cgacacatgc
    28321 tgactacttt catgctcaag ctgaccgata acctgccgcg cctgcgccat ccccacgcta
    28381 cctaagcgcc agtgtggttg ccctgcgctg gcgctaaatc ccggaatcgc tccctgtcag
    28441 tcaagattca gcttcagccg ctccgggcaa taaataatat tctgcaaaac cagatcgtta
    28501 acggaagcgt aggagtgttt atcgtcagca tgaatataaa agagatcgcc acgggtaatg
    28561 cgataaggac gatcgttgag tacatgcaga ccattaccgc gccagacaat caccagctca
    28621 caaaaatcat gtgtatgttc ggcaaagaca tcttgcggat aacggtcagc cacagcgact
    28681 gcctgctggt cgctggcaaa aaaatcatct ttgagaagtt ttaactgatg cgccaccgtg
    28741 gctacctcgg ccagagaacg aagttgatta ttcgcaatat ggcgtacaaa tacgttgaga
    28801 agattcgcgt tattgcagaa agccatcccg tccctggcga atatcacgcg gtgaccagtt
    28861 aaactctcgg cgaaaaagcg tcgaaaagtg gttactgtcg ctgaatccac agcgataggc
    28921 gatgtcagta acgctggcct cgctgtggcg tagcagatgt cgggctttca tcagtcgcag
    28981 gcggttcagg tatcgctgag gcgtcagtcc cgtttgctgc ttaagctgcc gatgtagcgt
    29041 acgcagtgaa agagaaaatt gatccgccac ggcatcccaa ttcacctcat cggcaaaatg
    29101 gtcctccagc caggccagaa gcaagttgag acgtgatgcg ctgttttcca ggttctcctg
    29161 caaactgctt ttacgcagca agagcagtaa ttgcataaac aagatctcgc gactggcggt
    29221 cgagggtaaa tcattttccc cttcctgctg ttccatctgt gcaaccagct gtcgcacctg
    29281 ctgcaatacg ctgtggttaa cgcgccagtg agacggatac tgcccatcca gctcttgtgg
    29341 cagcaactga ttcagcccgg cgagaaactg aaatcgatcc ggcgagcgat acagcacatt
    29401 ggtcagacac agattatcgg tatgttcata cagatgccga tcatgagcgc gtacgaaaca
    29461 gaccgtgcca ccggtgatgg tatagggctg cccattaaac acatgaatac ccgtgccatg
    29521 ttcgacaatc acaatttcat gaaaatcatg atgatgttca ggaaaatccg cctgcgggag
    29581 ccggggttct atcgccacgg acgcgttacc agacggaaaa aaatccacac tatgtaatac
    29641 ggtcatactg gcctcctgat gtcgtcaaca cggcgaaata gtaatcacga ggtcaggttc
    29701 ttaccttaaa ttttcgacgg aaaaccacgt aaaaaacgtc gatttttcaa gatacagcgt
    29761 gaattttcag gaaatgcggt gagcatcaca tcaccacaat gaagctaatt gtgaacatca
    29821 tcacgttcat ctttccctgg ttgccaatgg cccattttcc tgtcagtaac gagaaggtca
    29881 cgaattcagg cgctttttag actggtcgta atgaaattca gcaggatcac attatgacct
    29941 ttcgcaattg tgtcgccgtc gatctcggcg catccagtgg gcgcgtgatg ctggcgcgtt
    30001 acgagcgtga atgccgcagc ctgacgctgc gcgaaatcca tcgttttaac aatggcctgc
    30061 atagccagaa cggttatgtc acctggaatg tggatagcct ggaaagtgcc attcgccttg
    30121 gattaaacaa ggtgtgcgag gaagggattc gtatcgatag cattgggatt gatacctggg
    30181 gcgtggactt tgtgctgctc gaccaacagg gtcagcgtgt gggcctgccc gttgcttatc
    30241 gcgatagccg ctccaatggc ctaatggcgc aggcacagca acaactcggc aaacgcgata
    30301 tttatcaacg tagcggcatc cagtttctgc ccttcaatac gctttatcaa ctgcgtgcgc
    30361 tgacggagca acaacctgaa cttattccac acattgctca cgctctgctg atgccggatt
    30421 acttcagcta tcgcctgacc ggcaagatga actgggagta caccaacgcc acgaccacgc
    30481 aactggtcaa tatcaatagc gacgactggg acgagtcgct actggcgtgg agcggggcta
    30541 acaaagcctg gtttggtcgc ccgacgcatc cgggtaatgt cattgggcac tggatttgcc
    30601 cgcagggtaa tgagattccg gtggtcgccg ttgccagcca tgataccgcc agcgcggtta
    30661 tcgcctcgcc gttaaacggt tcacgcgctg cttatctctc ttctggcacc tggtcattga
    30721 tgggcttcga aagccagacg ccatttacca atgacacggc gctggcagcc aacataacca
    30781 atgaaggcgg ggcggaaggt cgctatcggg tgctgaaaaa tattatgggc ttatggctgc
    30841 ttcagcgagt gcttcaggag cggcaaatca acgatctccc ggcgcttatc gccgcgacac
    30901 aggcacttcc ggcctgccgc ttcatcatca atcccaatga cgatcgcttt attaatcctg
    30961 aggcgatgtg cagcgaaatt caggctgcgt gtcgggaaat ggcgcaaccg ctcccggaaa
    31021 gtgatgctga actggcgcgc tgcattttcg acagtctggc gctgctgtat gccgatgtgt
    31081 tgcatgagct ggcgcagcta cgcggtgaag atttctcgca actgcatatt gtcggcggcg
    31141 gctgccagaa cacgctgctc aaccagctat gtgccgatgc ctgcggtatt cgggtgatcg
    31201 ccgggcctgt tgaagcctcg acgctcggca atatcggcat ccagttaatg acgctggatg
    31261 aactcaacaa tgtggatgat ttccgtcagg tcgtcagcac caccgcgaat ctgaccacct
    31321 ttacccctaa tcctgacagt gaaattgccc actatgtggc gcagcttcac tcgacacgac
    31381 agacaaagga gctttgcgca tgaccactca actggaacag gcctgggagc tggcgaaaca
    31441 gcgtttcgcg gcggtgggga ttgatgtcga ggaggcgctg cgccaacttg atcgtttacc
    31501 cgtttcaatg cactgctggc agggcgatga tgtttccggt tttgaaaacc cggaaggttc
    31561 gctgaccggt gggattcagg ctattggtaa ttatccgggc aaagcgcgta atgccagtga
    31621 gctacgagcc gatctggaac aggctatgcg gctgattccg gggccgaaac ggcttaattt
    31681 acatgccatc tatctggaat cagacacgcc agtctcgcgc gaccagatca aacctgagca
    31741 cttcaaaaac tgggttgaat gggcgaaagc caatcagctc ggtctggatt ttaacccctc
    31801 ctgtttctcg catccgctaa gcgccgatgg ctttacgctc tcgcatcccg acgacagcat
    31861 tcgccagttc tggattgatc actgcaaagc cagccgtcgc gtttcggcct attttggcga
    31921 gcaactcggc acaccatcgg tgatgaacat ctggatcccg gatggtatga aagatatcac
    31981 cgttgaccgt ctcgccccgc gtcagcgtct gctggcagca ctggatgagg tgatcagcga
    32041 gaagctggat ccggcgcacc atatcgacgc cgttgagagc aaattgtttg gcattggcgc
    32101 agagagctac acggttggct ccaatgagtt ttacatgggg tatgccacca gccgccagac
    32161 tgcgctgtgc ctggacgccg ggcatttcca cccgactgaa gtgatttccg acaagatttc
    32221 cgccgccatg ctgtatgtac cgcagttgct gctgcacgtc agccgtccgg ttcgctggga
    32281 cagcgatcac gtggtgctgc tggatgatga aacccaggcg attgccagtg agattgttcg
    32341 tcacgatctg ttcgaccggg tgcatatcgg cctcgacttc tttgatgcct ctatcaaccg
    32401 cattgccgcg tgggtcattg gtacacgcaa tatgaaaaaa gccctgctgc gtgcgttgct
    32461 ggaacctacc gctgagctgc gcaagctgga agcggcgggc gattacactg cgcgtctggc
    32521 gctactggaa gaacagaaat cgttgccgtg gcaggcggtc tgggaaatgt attgccaacg
    32581 tcacgatacg ccagcaggta gcgaatggct ggagagcgtg cgggcttatg agaaagcgat
    32641 tttgagccag cgtgggtaaa cactgccgga tgcggcgcga gcgccttatc cggcctacag
    32701 gtcgaatgac catgcgtgct gccaccctca ccccggccct ctccctgaaa gggcgaggga
    32761 gaaaaacata gcctcgtgcg gcaacggagc tgaacaccgc tccggacagt cccctcgccc
    32821 ctacggggag agggttaggg tgaggggaac aagccgcacc atgtctaaac aaggcaacct
    32881 aatttacagg aacacagaac atgcaaaaca ttacccagtc ctggtttgtc cagggaatga
    32941 tcaaagccac caccgatgcc tggctgaaag gctgggacga gcgcaatggt ggtaacctga
    33001 cgctgcgcct ggatgacgcc gatatcgcgc cttatcacgg caactttcat gcacaaccgc
    33061 gctatattcc cctcagccaa cccatgcctt tactggcaaa tacgccgttt attgtcaccg
    33121 gctccggcaa attcttccgt aacgtccagc ttgatcctgc agctaactta ggcgtcgtaa
    33181 aagtcgacag cgacggcgcg ggctaccata ttctctgggg gttaaccaac gaagccgttc
    33241 caacttccga acttccggct cacttccttt cccactgcga gcgcattaaa gccaccaacg
    33301 gtaaagatcg ggtgatcatg cactgccacg ccaccaacct gatcgccctc acctatgtac
    33361 ttgaaaacga caccgcggtc tttactcgcc aactgtggga aggcagcacc gagtgtctgg
    33421 tggtattccc ggatggcgtt ggcattctgc cgtggatggt gcccggcacg gacgaaatcg
    33481 gccaggcgac cgcacaagag atgcaaaaac attcgctggt gttgtggccc ttccacggcg
    33541 tcttcggcag cgggtcgacg ttggatgaaa cattcggttt aatcgacacc gcagaaaaat
    33601 cagcacaagt attagtgaag gtttattcga tgggcggcat gaaacagacc atcagccgtg
    33661 aagagttgat agcgctcggc aagcgcttcg gcgttacacc actagctagt gcgctggcgc
    33721 tgtaaggagc aaacatgatc cgcaaagcct ttgtcatgca ggttaacccc gacgcccacg
    33781 aagagtatca gcgtcggcat aaccccatct ggccggaact ggaagcagtg ttgaaatctc
    33841 acggtgcgca taactacgcc atttacctcg acaaagcgcg taatctgctg tttgccacgg
    33901 tagagattga atctgaagaa cggtggaatg cggtagccag cactgatgtt tgccaacgtt
    33961 ggtggaaata tatgaccgat gttatgcccg ctaacccgga taacagcccg gtgagtagcg
    34021 agctgcaaga agtgttttac ctgccgtaat tgcccaatgt gccagagatg cctccggcac
    34081 aatattttaa atttcgattt gaaaaattaa acatattaca tctcccggta agttatattt
    34141 ccctgataca ttgtgagtaa atcacaaaaa taatgaataa cccattaatg attcatgtgg
    34201 tttatttaaa taaccattat gtgcattact ccgcaaatct gacctttcac tctgtctcaa
    34261 attgtcattg tttaccgaca tatcgcctct catcacttaa tcgctccacc attacaattg
    34321 attaaaatat attttatgga gtccgattaa tggcagctct tactgcaagc tgtattgacc
    34381 tgaatattca gggcaatggc gcttattccg ttctgaagca gttggcgaca atagcgttac
    34441 aaaacggttt tatcaccgac tcacaccagt tcctgcaaac cctgctgctg cgcgaaaaaa
    34501 tgcactctac tggatttggt tccggtgtcg ccgtgccgca cggtaaaagc gcctgcgtta
    34561 aacaaccgtt tgtattattc gcccgcaaag cgcaggctat tgactggaaa gccagcgatg
    34621 gcgaagacgt caattgctgg atctgcctcg gcgtgccgca aagcggcgaa gaggatcagg
    34681 tcaaaatcat cggcacactg tgtcgcaaaa ttatccacca ggattttatt catcaactgc
    34741 aacagggcga cgccgaccag gtgcttgcct tgttaaatca aaccctcagc tcataaggaa
    34801 gtggcgatgg agtcatcctt atgtattgtc gcgatcacca actgccccgc cgggatcgct
    34861 cacacctaca tggtggcgga agccctggaa cagaaagccc gttctctcgg ccataccata
    34921 aaagtggaaa ctcaagggtc cagtggcgtt gaaaaccgct tatccagcga agagattgcc
    34981 gctgccgatt acgtcattct cgctaccggg cgtggcctga gcggtgatga tcgcgcgcgg
    35041 tttgccggga agaaagttta tgagattgcc atctcccagg cgttgaaaaa tatcgaccag
    35101 attttcagcg aattaccgac aaactcgcag ctttttgccg cagatagcgg cgtgaagctt
    35161 ggcaaacagg aagtgcaaag cggcagcgtg atgagccacc tgatggcggg cgtctctgcg
    35221 gcgctgccgt ttgtcatcgg cggtggtatc ctggtggcgc ttgccaacat gctggtgcag
    35281 ttcggtttgc cgtataccga tatgtcgaaa ggcgcgccgt cgtttacctg ggtagttgaa
    35341 tccatcggtt accttggctt cacctttatg atccccatca tgggcgctta tattgcctcg
    35401 tcgattgccg ataaaccggc tttcgctccg gcgtttctgg tttgctatct ggcgaacgac
    35461 aaagcgctgc tcggcaccca gtcgggtgcg ggattcctcg gcgcggtggt gctggggctg
    35521 gcgattggct atttcgtctt ctggttccgc aaagtgcgcc ttggcaaagc attacaaccg
    35581 ctgctcggtt caatgctgat cccgttcgtt actctgctgg ttttcggcgt gctcacttac
    35641 tacgttatcg ggccggtgat gtccgacctc atgggcgggc tgctccactt cctgaatacc
    35701 attcctccat caatgaagtt tgcggcagcg tttctggtcg gtgcgatgct ggcgttcgat
    35761 atgggcggcc ccatcaataa aaccgcctgg ttcttctgct tctcactgct ggaaaaacac
    35821 atttatgact ggtacgccat cgtcggcgtt gtcgcgttga tgccgcccgt tgcggcgggt
    35881 cttgcgacgt tcattgcgcc caaactgttt acccgacagg aaaaagaggc tgccagtagt
    35941 gccattgtgg tcggtgccac agtcgcaacc gaaccggcta taccttacgc tctggcagct
    36001 ccgttgccga tgattaccgc caatacgctg gcgggtggca ttaccggcgt gctggtgatc
    36061 gctttcggaa tcaaacgtct ggcacctggt ttgggtatct ttgacccgct gatcggcctg
    36121 atgtcgccgg tgggttcgtt ttatctggtg ctagcgattg gcctcgcgct gaacatttca
    36181 ttcattatcg tcctgaaagg attgtggtta cgccgcaaag cgaaagccgc gcaacaggag
    36241 cttgtccatg aacattgagt tactgcaaca gttgtgcgaa gccagcgccg tcagcggcga
    36301 tgaacaggaa gttcgcgaca ttctgataaa cacgctggaa ccttgcgtta atgagatcac
    36361 ctttgatggt ctgggcagct ttgttgcccg taaggggaat aaaggtccaa aagttgccgt
    36421 tgtcaggcat atggatgaag tcggctttat ggtcacccac atcgacgaga gcggttttct
    36481 gcgctttacc accattggcg gctggtggaa tcagtcaatg ctcaaccacc gggtaaccat
    36541 acgcacacac aagggattta aaatccctgg tgtgattggt tccgtcgcac ctcatgcgtt
    36601 aacggaaaag caaaagcaac agccgctgtc atttgatgag atgttcattg atattggcgc
    36661 gaacagtcgc gatgaggtgg aaaaacgcgg tgtggcgatg ggcgatttta ttagcccgga
    36721 agccaatttt gcctgctggg gcgaagataa agtggtcggc aaggcgctgg ataaccgcat
    36781 cggctgcgca atgatggctg aactattgca gacggtgaat aatcccgaaa ttacgctgta
    36841 tggcgtcggc agcgtggaag aagaagttgg gctacgcggg gcacaaacct cggcggaaca
    36901 cattaaaccg gatgtggtga ttgtgctgga taccgctgtc gcaggtgatg ttccgggcat
    36961 tgataacatt aaatacccgc tgaaactggg ccaggggccg gggctgatgc tgtttgacaa
    37021 gcgctacttc cccaaccaga aactggtggc ggcgttaaaa agctgtgccg cacataacga
    37081 tttaccgctg caattttcca ccatgaaaac cggtgcgacg gatggcgggc gctacaacgt
    37141 gatgggcggc gggcgtccgg ttgttgcgct gtgcctgcca acccgctatc tgcacgctaa
    37201 cagcggcatg atttcaaaag ccgattacga tgctctgctc acgttgatac gggattttct
    37261 gaccacctta accgcggaga aagtcaacgc gtttagccag ttccgtcagg tggattaatg
    37321 ctaaacgaac gccagttaaa gattgtcgat ctgctggagc aacagccgcg cacgcctggt
    37381 gagctggcgc aacagactgg cgtttcaggc aggaccatcc tgcgtgatat tgactatctc
    37441 aactttaccc ttaacggcaa agcccgcatt ttcgccagtg gcagtgcggg ctatcagctg
    37501 gaaatcttcg agcgccgcag cttttttcag ttgctgcaaa agcacgataa cgacgatcgg
    37561 ctgctggcgc tgttattact gaataccttt actccccgtg cgcaactcgc ctcggcgctt
    37621 aatttgccag aaacctgggt agcagagcgt ctgccccggt taaaacagcg ttatgaacgc
    37681 acttgttgcc tggccagtcg ccccggtttg ggccatttca ttgatgagac agaagagaaa
    37741 cgcgttatct tgctggcgaa cttgctgcgc aaagatccgt ttttaattcc gctggcgggc
    37801 gtaacacgag acaattttca gcatttatcc acggcctgcg acaaccaaca ccgctggccg
    37861 ctcatgcagg gtgattatct ctccagcctg attctggcga tttacgccct gcgtaatcaa
    37921 ctgaccgatg agtggccgca atatcccggt aacgagataa aacaaatcgt tgaacagagc
    37981 ggcatgtttc ttggtgataa cgctgtaaga accctgacag gtttgataga gaaacagcat
    38041 cagcaagcgc aggtaatttc agccgatcat gtgctggggt tgctgcaaag ggtgccaggc
    38101 atcgcgtcat tgaatattat tgatacgcag ctggttgaga atattaccgg gcatttatta
    38161 cgttgccttg ccgcaccggt gtggattgcc gagcaccgcc aaagcagcat gaataacctg
    38221 aaagccgcct ggcctgcggc ctttgatatg agtctgcact ttattacgct actgcgtgaa
    38281 cagctcgata ttcccctttt cgatagcgat ctgatcggtt tgtattttgc ctgtgcgctg
    38341 gaacggcatc aaaacgaacg ccagccgatc attttgctct cggaccagaa cgcgattgcc
    38401 actattaatc agctcgccat tgagcgcgat gttttacatt gtcgggtgat tattgcccgt
    38461 agcttaagcg agcttattgc cattcgcgaa gagattgagc cgttattgat cattaacaac
    38521 agccattatt tactggatga cgcggtaaat aattacatca ccgtaaaaaa tatcattacg
    38581 gctgccgata tcgaacaaat aaaacacttt ctggcgacgg catttattcg ccaacagccg
    38641 gaacgctttt tctctgcccc cggaagtttt cattattcga atgtacgcgg tgaaagctgg
    38701 caacatatta cccggcaaat ttgtgcgcaa ttagtggcac aacaccatat taccgccgat
    38761 gaagcgcaac ggatcatcgc ccgcgaaggc gaaggtgaaa acctgattgt taatcgcctc
    38821 gcaatcccac attgctggag cgaacaggag cgacgttttc gtggattttt tattaccctc
    38881 gcccaaccag ttgaggtgaa taacgaagtc attaaccatg tcttgatcgc ctgcgccgcc
    38941 gccgatgcgc gtcatgagct aaaaatattt agctatctgg caagcgtatt gtgtcagcat
    39001 ccggcagaag ttattgccgg gttaacagga tatgaggcat ttatggagtt acttcacaag
    39061 gggtgaactg tgtcggatgc gccgcatccg acactgcatt ttcatcaaaa acgtttaacg
    39121 caggctatta tagtcatcaa ccatttcgtt gtactcgcgt aacgctcgtg gaaatgagtc
    39181 ttcgaccatc cagcttgtgt ttgcatcctg aagatgctct ttatcccagt cagagtacgg
    39241 gactttatct ctgacgcggc gaacatattt tttagtctcg agttgatact ggtctgccga
    39301 attaatgaac gagaaattca taccgccttt atccgcttct ttggcctgcg ctaccagcgt
    39361 ttccagttca gagactttct tcattgccgc atcaacatca a
//
LOCUS       DAJTZL010000017        39255 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.017, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000017 DAJTZL010000000
VERSION     DAJTZL010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39255
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.017"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..1112)
                     /gene="setA"
                     /locus_tag="OO544_000776"
     CDS             complement(<1..1112)
                     /gene="setA"
                     /locus_tag="OO544_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HCR6766451.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLPSVARVELPSENALSMQGGWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSELGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVAAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNSISTGVILAGVIQGAIAQSWGHFAV"
     gene            complement(1214..1345)
                     /gene="sgrT"
                     /locus_tag="OO544_000777"
     CDS             complement(1214..1345)
                     /gene="sgrT"
                     /locus_tag="OO544_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HCR6766452.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            1434..3089
                     /gene="sgrR"
                     /locus_tag="OO544_000778"
     CDS             1434..3089
                     /gene="sgrR"
                     /locus_tag="OO544_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HCR6766453.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     TTNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      3123..3222
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3253..4236
                     /gene="thiB"
                     /locus_tag="OO544_000779"
     CDS             3253..4236
                     /gene="thiB"
                     /locus_tag="OO544_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HCR6766454.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFTFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVYPAFQNAIP
                     TGNWMYPVANVTQPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            4212..5822
                     /gene="thiP"
                     /locus_tag="OO544_000780"
     CDS             4212..5822
                     /gene="thiP"
                     /locus_tag="OO544_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HCR6766455.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALIQMMCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQSVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDISARYSMLCQSLGIEGWSRL
                     NVVELRALKRPLVQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            5806..6504
                     /gene="thiQ"
                     /locus_tag="OO544_000781"
     CDS             5806..6504
                     /gene="thiQ"
                     /locus_tag="OO544_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HCR6766456.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDSVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTDELL
                     SGKASASALLGITG"
     gene            complement(6574..7338)
                     /locus_tag="OO544_000782"
     CDS             complement(6574..7338)
                     /locus_tag="OO544_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR6766457.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWMAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(7424..8302)
                     /gene="araC"
                     /locus_tag="OO544_000783"
     CDS             complement(7424..8302)
                     /gene="araC"
                     /locus_tag="OO544_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HCR6766458.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRA
                     YWHEWLNWPPIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            8641..10341
                     /gene="araB"
                     /locus_tag="OO544_000784"
     CDS             8641..10341
                     /gene="araB"
                     /locus_tag="OO544_000784"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HCR6766459.1"
                     /translation="MAIAIGLDFGSDSVRALAVECATGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKIVLAELSAEQRAAVVGIGVDTTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATNAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            10352..11854
                     /gene="araA"
                     /locus_tag="OO544_000785"
     CDS             10352..11854
                     /gene="araA"
                     /locus_tag="OO544_000785"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HCR6766460.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            12023..12718
                     /gene="araD"
                     /locus_tag="OO544_000786"
     CDS             12023..12718
                     /gene="araD"
                     /locus_tag="OO544_000786"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HCR6766461.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQSGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            12793..15144
                     /gene="polB"
                     /locus_tag="OO544_000787"
     CDS             12793..15144
                     /gene="polB"
                     /locus_tag="OO544_000787"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HCR6766462.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILQGKQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPQLLEKLNAWFANYDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHTSPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRETIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            15308..18214
                     /gene="rapA"
                     /locus_tag="OO544_000788"
     CDS             15308..18214
                     /gene="rapA"
                     /locus_tag="OO544_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HCR6766463.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPVQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRMEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            18226..18885
                     /gene="rluA"
                     /locus_tag="OO544_000789"
     CDS             18226..18885
                     /gene="rluA"
                     /locus_tag="OO544_000789"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HCR6766464.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(19002..19817)
                     /gene="djlA"
                     /locus_tag="OO544_000790"
     CDS             complement(19002..19817)
                     /gene="djlA"
                     /locus_tag="OO544_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HCR6766465.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            20072..22426
                     /gene="lptD"
                     /locus_tag="OO544_000791"
     CDS             20072..22426
                     /gene="lptD"
                     /locus_tag="OO544_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HCR6766466.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWQHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            22479..23765
                     /gene="surA"
                     /locus_tag="OO544_000792"
     CDS             22479..23765
                     /gene="surA"
                     /locus_tag="OO544_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HCR6766467.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            23765..24754
                     /gene="pdxA"
                     /locus_tag="OO544_000793"
     CDS             23765..24754
                     /gene="pdxA"
                     /locus_tag="OO544_000793"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HCR6766468.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTAGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLNELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            24751..25572
                     /gene="rsmA"
                     /locus_tag="OO544_000794"
     CDS             24751..25572
                     /gene="rsmA"
                     /locus_tag="OO544_000794"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HCR6766469.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            25575..25952
                     /gene="apaG"
                     /locus_tag="OO544_000795"
     CDS             25575..25952
                     /gene="apaG"
                     /locus_tag="OO544_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HCR6766470.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            25959..26801
                     /gene="apaH"
                     /locus_tag="OO544_000796"
     CDS             25959..26801
                     /gene="apaH"
                     /locus_tag="OO544_000796"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HCR6766471.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGTLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(26879..27358)
                     /gene="folA"
                     /locus_tag="OO544_000797"
     CDS             complement(26879..27358)
                     /gene="folA"
                     /locus_tag="OO544_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HCR6766472.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(27550..29412)
                     /gene="kefC"
                     /locus_tag="OO544_000798"
     CDS             complement(27550..29412)
                     /gene="kefC"
                     /locus_tag="OO544_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HCR6766473.1"
                     /translation="MDSHTLIQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLHWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLATSSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLLENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGTAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(29405..29935)
                     /gene="kefF"
                     /locus_tag="OO544_000799"
     CDS             complement(29405..29935)
                     /gene="kefF"
                     /locus_tag="OO544_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HCR6766474.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGKALHGKYLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(30043..31374)
                     /locus_tag="OO544_000800"
                     /pseudo
     CDS             complement(30043..31374)
                     /locus_tag="OO544_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414587.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(31433..31720)
                     /gene="fixX"
                     /locus_tag="OO544_000801"
     CDS             complement(31433..31720)
                     /gene="fixX"
                     /locus_tag="OO544_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HCR6766475.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQALELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(31717..33003)
                     /locus_tag="OO544_000802"
     CDS             complement(31717..33003)
                     /locus_tag="OO544_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR6766476.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFAESAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(33054..33995)
                     /locus_tag="OO544_000803"
     CDS             complement(33054..33995)
                     /locus_tag="OO544_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HCR6766477.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINTFVLNDADGAQA
                     IQLGANHVWKLNGKPDDRMIEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LKAAVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(34010..34780)
                     /locus_tag="OO544_000804"
     CDS             complement(34010..34780)
                     /locus_tag="OO544_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HCR6766478.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAEAA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            35254..36768
                     /gene="caiT"
                     /locus_tag="OO544_000805"
     CDS             35254..36768
                     /gene="caiT"
                     /locus_tag="OO544_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiport BCCT
                     transporter"
                     /protein_id="HCR6766479.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVLGLTASTWILWTVLGSNTLLLIDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            36799..37937
                     /gene="caiA"
                     /locus_tag="OO544_000806"
                     /pseudo
     CDS             36799..37937
                     /gene="caiA"
                     /locus_tag="OO544_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
     gene            38066..39255
                     /gene="caiB"
                     /locus_tag="OO544_000807"
                     /pseudo
     CDS             38066..39255
                     /gene="caiB"
                     /locus_tag="OO544_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
BASE COUNT         9394 a        10791 c        10280 g         8790 t
ORIGIN      
        1 acagcaaagt gcccccaact ttgtgcaatt gctccctgaa taacgccagc cagaattacc
       61 ccggtagaaa tactgttagt aaataaggtg gtagctgccc ccgctcttcc aggcattaaa
      121 tcctgaaacc atagcatccc aatacccgca acaatgccga taaatacagc gttaaaaagt
      181 tgcagcgtca tcaacgccat acggctatga aagaaaatca atccggtgta aaacagtact
      241 cctgccgcca ctgctatgac catcattcgc cgcttaccat aacgtttgac atagtagcca
      301 gccagaatca ttgctggtat ttccagtcca gctgccgtcc ccatcaggaa acccgccagt
      361 ttgtctggca atcctaactc gctactgatc cacaacggca tatcaataat gtacatggtg
      421 ttgcaggtcc acattaacgt cgaggcgaca aataacatcc gtacgttact atcctgccag
      481 ccaccttgca ttgataaagc attttccgac ggcagttcta cccgcgccac agacggaagc
      541 ataaatgcaa tcaataccag actgagtgtg aatatcccgg cggcaatcga aaacatcacc
      601 gtaaagccgt aattcaacgc cagcataaag gccaacggtg gaccgataac ccatgccaga
      661 gaaagctgcg cacgcatcac cgagctaaac atcaccactt ctcgcgccga gttatccgca
      721 tattcccgcg ccagagcaaa taactgtggc attgccgtat tggccagaga tgccagaagc
      781 acaccacagg tgataagcgt cagataatga cgattaaatg caaacaatag cgcattgccg
      841 atagccatca aacagcaaaa tataatcagt tttcgccgat cgccctgact gtcagaacgt
      901 tttgccaacc agaggcttac gccgatccca gcaatagcat tcaccgtata aaagagtccg
      961 atccagaaag gttgcgcgcc aacctcacga ctcagaaata agctcaatgt aggagcctgt
     1021 agcgccccgg ccacccccat cataaaagcg accagcataa atgccgcgta aacaccgttc
     1081 atacggcgag ccatcgtcat tatccagatc atacgttccc tttttagcgc ggcgagaata
     1141 aaaaaaacca gcaggtataa tctgctggcg ggtgatttta caccaatact cagtcacaca
     1201 tgatgcaggc aagtcaactt tcagaattgc ggtcatccca ctgcatcagt ccttcaagca
     1261 tggttaatcg ttgtgggacg cttaaccaac gcaaccagca caacttcgct gtcgcggtaa
     1321 aatagtgctg ataaaactga cgcatggggc acctccttgc ttcatcgtta ggaattattg
     1381 acttaatata gggaaaataa aattgctgtc ttttgcgcag gagttcccct tttatgccat
     1441 ctgctcgtct gcaacaacag ttcatccgcc tgtggcaatg ctgcgagggt aaatcgcagg
     1501 acacaacgct caacgaactg gcagcgttat tgagctgctc gcgtcgtcat atgcgtaccc
     1561 tgctcaacac catgcaggat cgcggctggc tgacgtggga agcggaagtc gggcgcggta
     1621 aacgctcgcg tctgacattc ctctataccg ggctggcgct tcagcaacag cgggcggaag
     1681 acctgctgga gcaggatcgt atcgaccaac tagtgcagtt ggttggcgac aaagcgactg
     1741 tgcggcaaat gctggtttct catctgggcc gcagcttccg ccaggggcgg cacatcctgc
     1801 gcgtgctcta ctatcgtccg ttgcgtaatc tgctacctgg cagcgcattg cgccgttccg
     1861 aaacccatat tgcccggcaa atcttcagtt ccctaacgcg cataaatgag gaaaatgggg
     1921 aactggaagc agacatcgcc caccactggc agcaaatatc accgcttcac tggcgtttct
     1981 ttttgcgtcc aggagtccat tttcatcatg gtcgtgaact ggaaatggac gacgtgatcg
     2041 cctctttaaa acgaatcaat acgctgccgc tctattcgca tattgcggac attgtgtcgc
     2101 cgacgccctg gacgctggat atccatctca cgcaaccgga ccgctggtta ccgttactgc
     2161 tggggcaagt tccggcgatg atcctgccgc gcgaatggga aaccctcagt aactttgcca
     2221 gccatcccat cggcaccggt ccgtatgcgg tgatccgcaa caccaccaat caactgaaaa
     2281 ttcaggcatt cgatgacttc ttcggttacc gggcattaat cgatgaagtt aacgtctggg
     2341 ttctgccgga aattgccgac gagccagctg gaggtctgat gctaaaaggt ccacagggtg
     2401 aggaaaaaga gattgaaagc cgcctggagg aaggttgcta ctatttacta ttcgacagcc
     2461 gcacccatcg cggggcgaat cagcaagtca gggactgggt aagctatgtg ctttctccaa
     2521 ctaatctggt ctatttcgct gaggaacagt accagcaact gtggttcccg gcttatggac
     2581 tgctcccccg ttggcaccat gcccgcacca taaagagcga aaaaccggct ggcctggaaa
     2641 gcctcaccct gaccttttat caggatcaca gtgagcatcg ggtgattgcc gggatcatgc
     2701 agcagattct ggcaagtcac caggtcacgc tggaaatcaa agagatcagc tacgatcagt
     2761 ggcatgaagg agagatcgag agcgatatct ggcttaacag cgccaacttt acgctgccgc
     2821 tggatttttc gctgttcgcg cacttgtgcg aggtgccgct gctccaacac tgtattccga
     2881 tcgactggca agccgacgcc gcccgctggc gcaatggcga aatgaacctg gcgaactggt
     2941 gccagcaact ggtcgccagc aaagcaatgg tgccgcttat ccaccactgg ctgatcattc
     3001 aggggcaacg cagtatgcgc ggcctgcgca tgaacaccct cggctggttc gattttaaat
     3061 cagcgtggtt tgcgccaccg gatccatgat tgctggtaga taacaaaatc actacactaa
     3121 cgccgttctc aacggggtgc cacgcaaacg cgtgcgctga gaaaataccc gtcgaacctg
     3181 atccggataa cgccgacgaa gggatttgag gctccttctc aagtcctttg ccactctttt
     3241 ttgaggtgca aagtgttaaa aaaatgtctt cccctgctgt tgctgtgcac agcgcccgtt
     3301 ttcgctaaac ccgttctgac tgtttatacc tacgattcct tcgccgccga ctgggggcct
     3361 ggtccaaaga tcaaaaaagc ttttgaagcc gactgtaact gcgaactgaa actggtggcg
     3421 ctggaagatg gcgtttcgct tctcaaccgt ctacggatgg aaggcaaaaa tagtaaagcc
     3481 gatgtggtgc tggggctgga taacaatctg ttagacgccg ccagtaaaac cggactattt
     3541 gccaaaagcg gtgtggcagc ggatgccgtt aacgttcccg gcggctggaa taatgacact
     3601 ttcgtcccgt ttgattacgg ctatttcacc ttcgtctatg acaagaacaa actgaaaaac
     3661 ccgccgcaaa gcctgaaaga attggttgag agcgatcaga actggcgggt gatttatcag
     3721 gatccgcgta ccagcacgcc ggggctgggt ctgctgttgt ggatgcaaaa agtctatggc
     3781 gatgacgccc cacaagcctg gcagaaactg gcgaagaaaa ctgtcacggt caccaaaggc
     3841 tggagcgaag cctatggcct gtttttaaaa ggcgaaagcg atctggtact gagttacacc
     3901 acctctccgg cttatcacat tctcgaagag aagaaagata actacgccgc cgcgaacttc
     3961 agcgaaggtc actatctgca ggtggaagtc gccgcccgca ccgctgccag caagcagccg
     4021 gagctggcgc aaaaattcct ccagtttatg gtttatccgg cttttcagaa tgcgatccca
     4081 accggcaact ggatgtatcc ggtggcaaac gtcacgcagc ctgccggttt tgaacaattg
     4141 accaaaccag caaccacgct ggagttcacg ccagccgaag tggcggcaca acgtcaggca
     4201 tggattagcg aatggcaacg cgccgtcagc cgttaattcc cggctggtta attccaggtg
     4261 taagcgccgc cacgctggtg gtagcggttg cgctcgcggc gtttctcgcc ctgtggtgga
     4321 acgcgccgca gggtaactgg gtagcagtct ggcaggacag ctacctgtgg catgtggtgc
     4381 gcttctcctt ctggcaggcg tttctctcgg cgctactctc tgtcgtaccc gcgatattcc
     4441 tcgcccgcgc cctctatcgc aggcgctttc cgggtcggct ggcgctgttg cgtctgtgcg
     4501 caatgacctt gatcctcccg gtgctggttg ctgttttcgg cattcttagc gtctatggtc
     4561 gtcagggctg gctggcatcg ctctgccaat cgctcggtct ggagtggacc ttttcgccct
     4621 acggcctgca aggcattttg ctggcccatg tgttttttaa tctgccgatg gcgagccgtt
     4681 tattacttca ggcactggaa aacattcccg gcgaacaacg tcaacttgcc gcccagcttg
     4741 gaatgcgtgg ctggcacttc tttcgtttcg tcgaatggcc gtggttacgg cgacaaatcc
     4801 cgccagttgc tgcgcttatc tttatgctct gtttcgccag cttcgccacc gtactgtcgc
     4861 taggcggcgg tccgcaagcg accactatcg agctggctat ttatcaggcg ctgagttacg
     4921 actacgatcc tgcccgcgcg gcgatgctgg cgctgatcca gatgatgtgt tgccttgggc
     4981 tggtgctgtt gagtcagcga ttgagtaagg ccattgcgcc cggcaccacg ctgctgcaag
     5041 gctggcgcga cccggacgat cgtctgcata gccgcatttg cgacacggtg ttaatagtgc
     5101 tggcgctgct gctgttgttg ccaccgttgc tggcggtgat cgtcgatggg gtaaatcgcc
     5161 agttgccgga agtgctggca caatcggtgc tgtggcaggc gctgtggacc tcgttgcgta
     5221 ttgcgctggc ggcaggtgta ttgtgcgtag tgctgaccat gatgctgcta tggagcagtc
     5281 gcgaacttcg ggcgcggcag aaaatgctgg cgggtcaggc gctggagatg agcggcatgt
     5341 tgatcctcgc catgccgggg attgtgctgg ctaccggctt ctttttactg ctcaacaaca
     5401 ctatcggcct gccacaatct gctgacggca ttgtgatttt caccaatgcg ttaatggcga
     5461 tcccttacgc gctgaaagta ctggaaaacc cgatgcgcga tatctccgcc cgctacagca
     5521 tgttgtgtca gtcgttgggc attgaaggct ggtcacgctt aaacgtggtg gagctgcgcg
     5581 ccctgaaacg cccactggtg caggcgctgg cctttgcatg cgtgctgtcg attggtgatt
     5641 ttggcgtggt ggcgttgttc ggtaacgatg atttccgcac cctgccgttt tatctctacc
     5701 agcaaattgg ctcctatcgc agccaggacg gcgcggtcac cgcgttaatt ctgctactac
     5761 tctgttttct gctgtttacc gtgattgaaa aactaccggg gcgaaatgtt aaaactgact
     5821 gatatcacct ggctttacca ccatttgccg atgcgtttta gcttaacggt agaacgcggc
     5881 gagcaggtgg cgatcctcgg gccaagcggc gcaggtaaaa gtaccctgct gaatttgatt
     5941 gccggttttc tgacgccagc cagcggttcg ctgactatcg acagcgttga tcacaccacg
     6001 acgccgccgt cacgccgtcc ggtatcgatg ctgtttcagg agaacaacct gttcagccac
     6061 ctgacggtcg cgcagaacat cgggctgggg cttaatccgg gattgaagct gaacgcggcg
     6121 cagcaggaga aaatgcacgc tatcgcccgc cagatgggga ttgataattt aatggcgcgg
     6181 ttaccgggcg agctttccgg cggtcagcga cagcgcgtgg cgttagcgcg ttgtctggta
     6241 cgcgagcagc cgattttatt gctcgatgaa ccgttctctg cgctcgatcc ggcgctacgc
     6301 caagagatgt tgacgctggt gagcacgagc tgccagcagc aaaaaatgac gttattgatg
     6361 gtgtcgcaca gtgtggaaga tgccgcgcgg atcgccacgc gctcggtggt ggtcgccgac
     6421 gggcgcatcg cctggcaggg taaaaccgat gaattgttaa gcggtaaggc gagtgcttcg
     6481 gcgctgttgg gaattacagg ttaaaagcgc acggacagtc ccctcgcccc tctggggaga
     6541 agggaacaaa ccagcgccga tgcaaactat cccctaaacc ccaaccactt tacgcaaaat
     6601 atcgatatac accggcatca acgggtggcg aattaacacc accagcgcca ccacgccgat
     6661 ggcagaaatc aacggcgtca gccacaacaa acgaccgcgg gacaaataat gactcaaacg
     6721 gtcagtcgct ttaccactgc gccataaccg ccagcacagc cagccaccaa cccacaaaaa
     6781 taccgccgtc gccagcagca accatttaaa ctcaccgctc tgcatcccgg caggaatatc
     6841 gatcgccgcg cccgccagaa tccctggcag gaagtaaaac ggcggccaca gcaggcagcc
     6901 gataatattc ggcgtgataa attttgccac tggcagatcc agcattcccg ccaccattgg
     6961 caccagcgga cgcgtcgggc caacaaaacg accgaccaga atggtgaaca tgctgtgttg
     7021 atgcaacgca tgttcagttt tatcaagtag tgctttgttt ttcttcagaa atgaccagcg
     7081 atgcaacggc tttttaaaac gccagcccag ccagaaggag atccagtcgc ccagcaggca
     7141 accgacaatc cccgccatcc acgcgtgcca gaaacttaac tcgccgctgc caatcagcgc
     7201 tcccagcccc gccatcagca ccgtaccggg tagaatcaaa ccgaccagcg ccagcgactc
     7261 cagaaaggcc accaacacca ccgccatcaa tgaatacacg gtggattggg taataaagtg
     7321 ttccagcaat gcttgcataa tgtgcctgtc aaatggacga agcagggatt ctgcaaaccc
     7381 tatgctactc cgtcaagccg tcaattgtct gattcgttac caattatgac aacttgacgg
     7441 ctacatcatt cactttttct tcacaaccgg cacggaactc gctcgggctg gccccggtgc
     7501 attttttaaa tacccgcgag aaatagagtt gatcgtcaaa accaacattg cgaccgacgg
     7561 tggcgatagg catccgggta gtgctcaaaa gcagcttcgc ctgactaatg cgttggtcct
     7621 cgcgccagct taagacgcta atccctaact gctggcggaa aagatgtgac agacgcgacg
     7681 gcgacaagca aacatgctgt gcgacgctgg cgatatcaaa attgctgtct gccaggtgat
     7741 cgctgatgta ctgacaagcc tcgcgtacac gattatccat cggtggatgg agcgactcgt
     7801 taatcgcttc catgcgccgc agtaacaatt gctcaagcag atttatcgcc agcagctccg
     7861 aatagcgccc ttccccttgc ccggcgttaa tgatttgccc aaacaggtcg ctgaaatgcg
     7921 gctggtgcgc ttcatctggg cgaaagaaac ccgtattggc aaatattggc ggccagttaa
     7981 gccattcatg ccagtaggcg cgcggacgaa agtaaaccca ctggtgatac cattcgcgag
     8041 cctccggatg acgaccgtag tgatgaatct ctcctggcgg gaacagcaaa atatcacccg
     8101 gtcggcagac aaattctcgc ccctgatttt tcaccacccc ctgaccgcga atggtgagat
     8161 tgagaatata acctttcatt cccagcggtc ggtcgataaa aaaatcgaga taaccgttgg
     8221 cctcaatcgg cgttaaaccc gccaccagat gggcgttaaa cgagtatccc ggcagcaggg
     8281 gatcattttg cgcttcagcc atacttttca tactcccgcc attcagagaa gaaaccaatt
     8341 gtccatattg catcagacat tgccgtcact gcgtctttta ctggctcttc tcgctaaccc
     8401 aaccggtaac cccgcttatt aaaagcattc tgtaacaaag cgggaccaaa gccatgacaa
     8461 aaacgcgtaa caaaagtgtc tataatcacg gcagaaaagt ccacattgat tatttgcacg
     8521 gcgtcacact ttgctatgcc atagcatttt tatccataag attagcggat cctacctgac
     8581 gctttttttc gcaactctct actgtttctc catacccgtt tttttggatg gagtgaaacg
     8641 atggcgattg caattggcct cgattttggc agtgattctg tgcgagcttt ggcggtggaa
     8701 tgcgctaccg gtgaagagat cgccaccagc gtagagtggt atccccgttg gcagaaaggg
     8761 caattttgtg atgccccgaa taaccagttc cgccatcatc cgcgtgacta cattgagtca
     8821 atggaagcgg cgctgaaaat cgtgcttgca gagcttagcg ccgaacagcg cgcagctgtg
     8881 gtcgggattg gcgttgacac aaccggctcg acgcccgcac cgattgacgc cgacggtaac
     8941 gtgctggcgc tgcgcccaga gtttgccgaa aacccaaacg cgatgttcgt attgtggaaa
     9001 gaccacactg cggttgaaga agcggaagag attacccgtt tgtgccacgc gccgggcaac
     9061 gttgactact cccgctatat tggcggtatt tattccagcg aatggttctg ggcaaaaatc
     9121 ctgcatgtga ctcgccagga cagcgccgtg gcgcaatctg ccgcatcgtg gattgagctg
     9181 tgcgactggg tgccagctct gctttccggt accacccgcc cgcaggatat tcgtcgcgga
     9241 cgttgcagcg ccgggcataa atctctgtgg cacgaaagct ggggcggctt gccgccagcc
     9301 agtttctttg atgagctgga cccgatcctc aatcgccatt tgccttcccc gctgttcact
     9361 gacacctgga ctgccgatat tccggtgggc accttatgcc cggaatgggc gcagcgtctc
     9421 ggcctgcctg aaagcgtggt gatttccggc ggcgcgtttg actgccatat gggcgcagtt
     9481 ggcgcaggcg cacagcctaa cgcactggta aaagttatcg gtacttccac ctgcgacatt
     9541 ctgattgccg acaaacagag cgttggcgag cgggcagtta aaggtatttg cggtcaggtt
     9601 gatggcagcg tggtgcctgg atttatcggt ctggaagcag gccaatcggc gtttggtgat
     9661 atctacgcct ggtttggtcg cgtactcggc tggccgctgg aacagcttgc cgcccagcat
     9721 ccggaactga aagcgcaaat caacgccagc cagaaacaac tgcttccggc gctgaccgaa
     9781 gcatgggcca aaaatccgtc tctggatcac ctgccggtgg tgctcgactg gtttaacggt
     9841 cgtcgcacgc caaacgctaa ccaacgtctg aaaggggtga ttaccgatct taacctcgct
     9901 accaacgctc cgctgctgtt cggcggtttg attgctgcca ccgcctttgg cgcacgcgca
     9961 atcatggagt gctttaccga tcaggggatc gccgtcaata acgtgatggc gctgggcggc
    10021 atcgcgcgga aaaaccaggt cattatgcag gcctgctgcg acgtgctgaa tcgcccgctg
    10081 caaattgttg cctctgacca gtgctgtgcg ctcggtgcgg cgatttttgc tgccgtcgcc
    10141 gcgaaagtgc acgcagacat cccatcagcc cagcaaaaaa tggccagtgc ggtagagaaa
    10201 accctgcaac cgcgcagcga acaggcacaa cgctttgaac agctttatcg ccgctatcag
    10261 caatgggcga tgagcgccga acaacactat cttccaactt ccgccccggc acaggctgcc
    10321 caggccgttg cgactctata aggacacgat aatgacgatt tttgataatt atgaagtgtg
    10381 gtttgtcatt ggcagccagc atctgtatgg cccggaaacc ctgcgtcagg tcacccaaca
    10441 tgccgagcac gttgttaatg cgctgaatac ggaagcgaaa ctgccctgca aactggtgtt
    10501 gaaaccgctg ggcaccacgc cggatgaaat caccgctatt tgccgcgacg cgaattacga
    10561 cgatcgttgc gctggtctgg tggtgtggct gcacaccttc tccccggcca aaatgtggat
    10621 caacggcctg accatgctca acaaaccgtt gctgcaattc cacacccagt ttaacgcggc
    10681 gctgccgtgg gacagtatcg atatggactt tatgaacctg aaccagactg cacatggcgg
    10741 tcgcgagttc ggcttcattg gcgcgcgtat gcgtcagcaa catgccgtgg ttaccggtca
    10801 ctggcaggat aaacaagccc atgagcgtat cggctcctgg atgcgccagg cggtctctaa
    10861 acaggatacc cgtcatctga aagtctgccg ttttggcgat aacatgcgtg aagtagcggt
    10921 caccgatggt gataaagttg ccgcacagat caagttcggt ttctccgtca atacctgggc
    10981 ggttggcgat ctggtgcagg tggtgaactc catcagcgac ggcgatgtta acgcgctggt
    11041 cgatgagtac gaaagctgct acaccatgac gcctgccaca caaatccacg gcgaaaaacg
    11101 acagaacgtg ctggaagcgg cgcgtattga gctggggatg aagcgtttcc tggaacaagg
    11161 tggcttccac gcgttcacca ccacctttga agatttgcac ggtctgaaac agcttccagg
    11221 tctggccgta cagcgtctga tgcagcaggg ttacggcttt gcgggcgaag gcgactggaa
    11281 aaccgccgcc ctgcttcgca tcatgaaggt gatgtcaacc ggtctgcagg gcggcacctc
    11341 ctttatggag gactacacct atcacttcga gaaaggtaat gacctggtgc tcggctccca
    11401 tatgctggaa gtctgcccgt cgattgccgt agaagagaaa ccgatcctcg acgttcagca
    11461 tctcggtatt ggtggtaagg acgatcctgc ccgactgatc ttcaataccc aaaccggtcc
    11521 agcgattgtc gccagcctga ttgatctcgg cgatcgttac cgtctgctgg ttaactgtat
    11581 cgacacggtg aaaacaccgc actccctgcc gaaactgccg gtggcgaatg cgctgtggaa
    11641 agcgcaaccg gatctgccaa ctgcttccga agcgtggatc ctcgctggtg gcgcgcacca
    11701 taccgtcttc agccatgcgc tgaacctcaa cgatatgcgc cagttcgccg agatgcacga
    11761 cattgaaatc acggtgattg ataacgacac ccgcctgcca gcgtttaaag acgcactgcg
    11821 ctggaacgaa gtgtattacg gatttcgtcg ctaagtagcc acatcaggta tgtaatgcct
    11881 gatgcgacgc tgacgcgtct tatctggcct acacgctgcg attttgtagg ccggataagc
    11941 aaagcgcatc cggcattgtt ttccccctcg cccctttggg gagagggccg gggtgagggg
    12001 aaacgacctc tcaggagtca acatgttaga agatctcaaa cgccaggtat tagaagccaa
    12061 cctggcgctg ccaaaacaca acctggtcac gctcacatgg ggcaacgtca gcgccgttga
    12121 tcgcgagcgc ggcgtctttg tgatcaaacc ttccggcgtc gattacagcg tcatgaccgc
    12181 tgacgatatg gtcgtggtta gcatcgaaac cggtgaagtg gttgaaggta cgaaaaagcc
    12241 ctcctccgac acgccaactc accggctgct ctatcaggca ttcccctcca ttggcggcat
    12301 tgtgcatacg cactcgcgcc acgccaccat ctgggcgcag tcgggtcagt cgattccagc
    12361 aaccggcacc acccacgccg actatttcta cggcaccatt ccctgcaccc gcaaaatgac
    12421 cgacgcagaa atcaacggcg aatatgagtg ggaaaccggt aacgtcatcg tagaaacctt
    12481 tgaaaaacag ggtatcgatg cagcgcaaat gcccggcgtt ctggtccatt cccacggccc
    12541 gtttgcatgg ggcaaaaatg ccgaagatgc ggtgcataac gccatcgtgc tggaagaggt
    12601 cgcttatatg gggatattct gccgtcagtt agcgccgcag ttaccggata tgcagcaaac
    12661 gctgctggat aaacactatc tgcgtaagca tggcgcgaag gcatattacg ggcagtaatg
    12721 actgtataaa accacagcca atcaaacgaa accaggctat actcaagcct ggttttttga
    12781 tggattttca gcgtggcgca ggcaggtttt atcttaaccc gacactggcg ggacaccccg
    12841 caagggacag aagtctcctt ctggctggcg acggacaacg ggccgttgca ggttacgctt
    12901 gcaccgcaag agtccgtggc gtttattccc gccgatcagg ttccccgcgc tcagcatatt
    12961 ttgcagggta aacaaggctt tcgcctgaca ccgctggcgt taaaggattt tcaccgccag
    13021 ccggtgtatg gcctttactg tcgcgcccat cgccaattga tgaattacga aaagcgcctg
    13081 cgtgaaggtg gcgttaccgt ctacgaggcc gatgtgcgcc cgccagaacg ctatctgatg
    13141 gagcggttta tcacctcacc ggtgtgggtc gagggtgata tgcacaatgg cactatcgtt
    13201 aatgcccgtc tgaaaccgca tcccgactat cgtccgccgc tcaagtgggt ttctatagat
    13261 attgaaacca cccgccacgg tgagctgtac tgcatcggcc tggaaggctg cgggcagcgc
    13321 atcgtttata tgctggggcc ggagaatggc gacgcctcct cgcttgattt cgaactggaa
    13381 tacgtcgcca gccgcccgca gttgctggaa aaactcaacg cctggtttgc caactacgat
    13441 cctgatgtga tcatcggttg gaacgtggtg cagttcgatc tgcgaatgct gcaaaaacat
    13501 gccgagcgtt accgtcttcc gctgcgtctt gggcgcgata atagcgagct ggagtggcgc
    13561 gagcacggct ttaaaaacgg cgtctttttt gcccaggcta aaggtcggct aattatcgac
    13621 ggtatcgagg cgctgaaatc cgcgttctgg aatttctctt cattctcgct ggaaactgtc
    13681 gctcaggagc tattaggcga aggaaaatct atcgataacc cgtgggatcg aatggacgaa
    13741 attgaccgcc gtttcgccga agataaacct gcgctggcaa cttataacct gaaagattgc
    13801 gagctggtga cgcagatctt ccacaaaact gaaatcatgc catttttact cgaacgagca
    13861 acggtgaacg gcctgccggt ggatcggcac ggcggttcgg tggcagcgtt tggtcatctc
    13921 tattttccgc gaatgcatcg cgctggttat gtcgcgccta atctcggcga agtgccgccg
    13981 cacaccagcc ctggcggcta cgtgatggat tcacggccag ggctttatga ttcagtgctg
    14041 gtgctggact ataaaagcct gtacccgtcg atcatccgca cctttctgat tgatcccgtc
    14101 gggctggttg aaggcatggc gcagcctgat ccagagcaca gtaccgaagg ttttctcgat
    14161 gcctggttct cacgggagaa acattgcctg ccggagattg tgactaacat ctggcacggg
    14221 cgcgatgaag ccaaacgtca gggtaataaa ccactttcgc aggcgctgaa gatcattatg
    14281 aatgcctttt atggcgtgct tggcaccacc gcctgccgct ttttcgatcc gcgcctggca
    14341 tcgtcgatca ccatgcgtgg tcatcagatc atgcggcaaa ccaaagcatt gattgaagcg
    14401 cagggctacg acgttatcta cggagatacc gactcgactt ttgtctggct aaaaggtgca
    14461 cattcggaag aagaggcggc gaaaatcggt cgtgcactgg tgcagcacgt taacgcctgg
    14521 tgggcggaaa cgctgcaaaa acaacggctg accagcgcat tagaactgga gtatgaaact
    14581 catttctgcc gttttcttat gccaaccatt cgcggagctg ataccggcag taaaaagcgc
    14641 tacgccgggc tgattcagga gggcgataaa cagcggatgg tgtttaaagg gctggaaacc
    14701 gtgcgcaccg actggacgcc gctggcccag cagtttcagc aggagctata cctgcgcatc
    14761 ttccgcaacg agccatatca ggaatatgta cgcgaaacca tcgacaaact gatggcgggt
    14821 gaactggatg cgcgactggt ttaccgtaaa cgccttcgcc gtccgctgag cgagtatcag
    14881 cgtaatgtgc cgcctcatgt acgcgccgct cgccttgccg atgaagaaaa ccaaaagcgt
    14941 ggtcgcccct tgcaatatca gaatcgcggc accattaagt acgtatggac caccaacggc
    15001 ccggagccgc tggactacca acgttcacca ctggattacg aacactatct gacccgccag
    15061 ctacaacccg tggcggaggg aatactccct tttattgagg ataattttgc tacacttatg
    15121 accgggcaac ttgggctatt ttgagcaaaa aaagagttcg ccagatacca ttttgatgcg
    15181 tgacgaatgc tttgccatcc agtaccatag cgccctttcc attcctggac ctgaataaca
    15241 ccactacccc ataagcacgg tagcgggtgg ttattgcctg caattaaaga tatagagccg
    15301 aacacatatg ccttttacac ttggtcaacg ctggatcagc gatacagaaa gcgaattggg
    15361 acttggaacc gttgtcgcgg tggatgcgcg aactgtcact ttacttttcc catctactgg
    15421 tgaaaaccgt ctgtacgcac gcagtgattc ccccgtgacc cgcgtgatgt tcaaccctgg
    15481 tgatactatt accagccatg acggctggca gatgcaagtc gaagaagtaa aagaagaaaa
    15541 tggcttgctg acctatatcg gtactcgcct ggatactgaa gagtccggcg tagccctgcg
    15601 tgaagttttc cttgatagca aactggtgtt cagcaaaccg caggaccgtc tgtttgccgg
    15661 gcagattgac cgtatggacc gctttgcgct gcgttatcgc gcgcgtaagt attccagcga
    15721 acagttccgt atgccgtaca gcggcctgcg cggtcagcgt accagcctga tcccgcatca
    15781 gctcaacatc gctcatgatg ttggtcgccg ccacgcgccg cgcgtcctgc tggctgacga
    15841 agtgggttta gggaaaacca ttgaagccgg gatgatcctg catcagcaac tgctctctgg
    15901 cgctgctgaa cgtgtgctaa ttatcgtccc ggaaacctta cagcatcagt ggctggtaga
    15961 aatgctgcgc cgtttcaacc tgcgctttgc gctatttgat gatgagcgtt atgccgaagc
    16021 tcagcacgat gcttacaacc cgttcgacac cgaacagctg gtgatttgct cgctggattt
    16081 tgcccgtcgt agcaaacagc gcctggaaca tctctgtgaa gccgaatggg acctgctggt
    16141 ggtcgatgaa gcgcatcacc tggtgtggag cgaagatgcg ccaagccgtg aatatcaggc
    16201 cattgaacaa ctggcagagc acgtgccggg cgttctgctg ctgaccgcga ccccggaaca
    16261 gctggggatg gaaagccact tcgcccgtct gcgtctgctg gacccgaacc gtttccacga
    16321 ttttgcgcag ttcgttgaag agcagaaaaa ttatcgtccg gttgcggacg ccgttgccat
    16381 gctgctggca ggtaacaaac tgagcaatga cgaactgaac atgctcggcg agatgatcgg
    16441 cgagcaggat atcgagccgc tgttgcaggc agcaaacagc gacagcgaag atgcccagag
    16501 cgcccgtcag gagctggttt cgatgctgat ggatcgccac ggcaccagcc gcgtgctgtt
    16561 ccgtaacacg cgtaacggtg tgaaaggatt cccgaaacgc gagctgcaca ccattaagct
    16621 gccgctgccg acgcagtatc agacggctat taaagtctcc ggcattatgg gcgcacgtaa
    16681 aagtgcggaa gatcgtgctc gcgatatgct ctacccggag cgtatttatc aggaatttga
    16741 aggtgataac gccacctggt ggaacttcga tccgcgcgtt gagtggctga tgggctacct
    16801 gaccagccat cgctctcaga aagtgctggt gatctgcgcc aaagctgcca ctgcgctgca
    16861 actggagcag gtactgcgcg aacgtgaagg tattcgcgct gcggtgttcc acgaaggtat
    16921 gtcgattatc gaacgtgacc gcgctgccgc ctggtttgcc gaagaagaca ccggcgcaca
    16981 ggtactgctg tgctcagaaa tcggttctga aggacgtaac ttccagttcg ccagccacat
    17041 ggtgatgttt gacctgccat tcaacccgga tctactggag cagcgtattg gtcgtctgga
    17101 tcgtatcggc caggcgcacg atattcagat ccatgtgcct tatctggaga aaaccgctca
    17161 gtcggtgctg gtgcgctggt atcacgaagg tctggatgca tttgagcaca cctgcccgac
    17221 cggacgcact atttacgata gcgtatacaa cgatctgatt aactatctgg cttcaccggt
    17281 tcaaaccgaa ggctttgacg atctgatcaa aaactgccgc gagcaacatg aagcgctgaa
    17341 agcacagctg gaacagggtc gtgaccgcct gctggaaatc cactccaacg gtggcgaaaa
    17401 agcccaggca ctggcagaaa gcattgaaga gcaggatgac gataccaacc tgatcgcctt
    17461 cgccatgaac ctgttcgata ttatcggtat caatcaggac gatcgcggcg acaacatgat
    17521 cgtgctgacg ccgtccgatc atatgctggt gccggacttc cctggcctgt cggaagatgg
    17581 catcaccatc acctttgatc gtgaagtggc gctggcgcgt gaagatgcac agtttattac
    17641 ctgggagcat ccgctgatcc gcaacggtct ggatctgatc ctttctggcg atactggtag
    17701 cagcacgatt tcactattga aaaacaaagc gttgccggta ggtacgctgt tggtggaact
    17761 gatttatgtc gttgaagccc aggctccgaa gcagttgcag ctcaaccgct tcctgccacc
    17821 gacgccggta cgtatgctgc tggataaaaa cggcaacaac ctggcggcgc aggtagagtt
    17881 tgaaaccttt aaccgtcagc ttaacgcggt taaccgtcac accggcagca aactggttaa
    17941 cgccgtgcag caggatgttc acgctatcct tcagctgggt gaagcgcaga tcgagaaatc
    18001 tgcccgtgca ttgattgatg ccgcacgtaa cgaagccgac gaaaaactgt ctgccgagct
    18061 gtctcgtatg gaagcgctgc gcgcagtgaa cccgaacatt cgtgacgacg aactgaccgc
    18121 cattgagagc aaccgtcagc aggtaatgga aagcctggat caggcaggct ggcgtctgga
    18181 tgccctgcgt ttgatcgttg taacgcatca gtaacggagc cgaaaatggg gatggaaaac
    18241 tacaatccac cgcaggaacc ctggttggtt atcctgtatc aggatgacca cattatggtg
    18301 gttaacaagc cgagcggttt gttgtctgtg ccggggcgtc tggaagagca caaagacagc
    18361 gtgatgacgc gcattcagcg tgattatccg caggcagaat cggtgcatcg tctggatatg
    18421 gctaccagcg gcgtgattgt agtggcgctg accaaagccg cggagcggga gttaaaacgc
    18481 cagttccgcg agcgcgagcc gaaaaagcag tatgtagccc gcgtctgggg gcatccatcc
    18541 cctgcggaag gtctggtgga tctgccgctg atttgcgact ggccaaaccg cccgaaacag
    18601 aaagtctgtt acgaaacggg taaacctgcg cagacggaat atgaagtggt ggagtatgcg
    18661 gcggataaca cggcaagagt ggtgttaaaa ccgattaccg ggcgttcgca tcagctgcgt
    18721 gtgcatatgc tggcgctggg tcatccgatt ctcggcgatc gtttttatgc atcaccagaa
    18781 gcgagagcga tggcaccgcg tttgttgcta catgcagaga tgttgacgat tacccatccg
    18841 gcgtatggca atagtatgac gtttaaagcg ccagcggatt tttaatcgtc acgctggcgc
    18901 agttcggtag gcggataagg cgttcacgcc gcatccggca attgtgcgcc gattgcctga
    18961 tgcgacgcgg gccgcgtctt atcaggccta cagtttaaaa cttatttaaa ccctttctgc
    19021 tgctttatca gctcatatgc ctgctgaatt tcctgcgctt tctgcttcgc catctccatc
    19081 atctcaggcg gcaaaccttt cgccaccagc ttatcgggat ggtgttcact catcagctta
    19141 cggtaggcac gtttgatggt ggtcgcatca tccgtcggct tcacgcccag cacattacag
    19201 gcatcttcca gcgtcgggcc acgctgcgct tgctgccagt taccaccgcc agattgctgc
    19261 tgataaccgc cgccaaactg tgcaccgccc tgcatcatgc gcaaaaactg gtcaaactgg
    19321 gcgcgggaga tccctaattc ttcagcaatg acatacagca ccgcccgttc attcgggtgc
    19381 agtgaaccat cagcaaacgc cgcctgaatc tggatttcca gaaacatacg aattaagtca
    19441 aaacgaccaa agcagacact gcgaaactgg cgcatctttt cgcgcagcgg gtaattatct
    19501 gattttccca cccggaacgc gttttgcgcc gcagtacggg aagcgccatg aaggttcatt
    19561 cggtccatca actggctggc gatatgaata tcagcctccg tgacgcgacc tttggatttg
    19621 gttaaatgcc ccatcacttc aaaagtggtg gcaaaaaaca gcgcctgacg ctcacgctgg
    19681 ttggcgaacc acgccatttt acggctacgg gctttatcaa acatatggcc aattaacagg
    19741 cctaacacta cgccccaaaa gccgccgccc atcagtaagg ccacggccac gccaatgatt
    19801 tttccccaat actgcatata ttccccaaat cgacacacgg atatcagggc tatctcccac
    19861 aatataaagg tgcttttacc gttttccggc tgcggtcaat tgtgggacat cgcctataat
    19921 ttgcattatc atacctgtca ttcaatgtcg tgcctaacac cactgacgct attcggacag
    19981 gattaacact agcgtagaga tgacgagtac gttagtctct gaccgtttgt cacgcgcaac
    20041 gttaccgatg atggaacaat aaaatcaacg tatgaaaaaa cgtatcccca ctctcctggc
    20101 caccatgatt gccaccgccc tttatagtca acagggactg gcagccgacc tcgcctcaca
    20161 gtgcatgttg ggcgtgccaa gctatgaccg tcctctggta cagggcgata ccaatgactt
    20221 acccgtgact atcaatgctg accacgcgaa aggggactac ccggatgacg ccgtgtttac
    20281 tggcagcgtg gatatcatgc agggtaacag ccgtctgcag gccgacgaag tgcagctcca
    20341 tcaaaaagag gcaccaggac aaccggagcc ggtacgtacc gttgatgcgc tcggtaatgt
    20401 ccattacgac gataaccagg tgatcctcaa agggccgaaa ggctgggcga atctgaacac
    20461 caaagatacc aacgtctggg aaggtgatta ccagatggtg ggtcgccagg gtcgcggtaa
    20521 agcggacctg atgaaacaac gtggcgaaaa ccgctatacc attctggata acggtagctt
    20581 tacctcctgt ctgccgggtt ctgacacctg gagcgtggta ggtagcgaaa ttattcatga
    20641 ccgcgaagaa caagttgcgg agatctggaa cgcccgcttt aaggtgggtc cggtaccgat
    20701 cttttatagc ccctatttgc agttgccggt gggtgacaaa cgtcgctctg gtttcttgat
    20761 cccgaacgcc aagtacacca ccaccaacta ctttgagttc tacctgccat attactggaa
    20821 catcgcgcca aatatggatg ccaccatcac gccgcattat atgcatcgtc gtggcaacat
    20881 catgtgggag aacgaattcc gctacctctc ccaggcgggc gctggcttga tggaactgga
    20941 ctatctgcct tcagataaag tctatgaaga tgaacacccg aacgatgaca gttcacgtcg
    21001 ttggttgttc tactggcaac actccggggt catggatcaa gtgtggcgtt tcaacgtcga
    21061 ctacaccaag gtcagcgatc ctagctactt caatgatttc gataacaagt acggttccag
    21121 tactgacggc tacgcaacgc aaaaattcag cgttggctat gcggtgcaaa acttcaatgc
    21181 caccgtttca accaagcagt tccaggtctt tagcgagcag aacaccagta gctactcggc
    21241 agagccgcag ttagacgtta actactacca gaatgatgtt ggtccgtttg atacgcgtat
    21301 ttacggccag gcagtgcact ttgttaacac cagagacgac atgcctgaag caacccgtgt
    21361 tcacctggaa ccgaccatca atttgccgct ctctaataac tggggcagca tcaataccga
    21421 agcgaagttg ctggcaaccc attatcagca aaccaatctt gactggtata actccagaaa
    21481 cacgaccaag ctggacgaat ccgttaaccg cgttatgccg caattcaaag ttgacggcaa
    21541 aatggtcttt gaacgcgata tggaaatgct ggctccgggt tatacccaaa cgctggaacc
    21601 gcgcgcgcag tatttgtacg tgccgtatcg cgatcagagc gacatctata actacgactc
    21661 gtctctactg caatctgact actctggcct gttccgggac cggacttacg gcggtcttga
    21721 ccgtattgcc tccgctaacc aggtgacgac cggtgtcaca tctcgcatat atgatgatgc
    21781 tgccgttgaa cgttttaata tttccgttgg tcaaatctac tatttcacgg agtctcgcac
    21841 tggcgatgac aacataacat gggagaatga cgacaaaacg ggctcactgg tgtgggcagg
    21901 cgatacttac tggcgtatct ccgagcgttg gggattgcgt ggcgggattc agtacgatac
    21961 acgtctggat aacgtagcga ccagtaactc cagcattgaa taccgtcggg atgaagaccg
    22021 tctggtacag ctgaattacc gttacgccag cccggaatat attcaggcta cgctgcctaa
    22081 gtactattcc actgctgagc aatataagaa tggtatttcg caggtaggtg ctgtcgccag
    22141 ctggccaatt gccgatcgtt ggtccattgt tggggcctac tactacgaca ccaatgcgaa
    22201 caagcaagcc gactctatgt taggtgtgca atacagctcc tgctgctatg caattcgcgt
    22261 cggttacgag cggaagctga acggttggga taacgataaa caacatgcgg tatatgacaa
    22321 cgcaatcggc tttaacatcg aacttcgcgg cctgagctcc aactacggtc tgggtacgca
    22381 agagatgctg cgttcgaaca ttctgccgta tcaaaacact ttgtgatttg ttgatttacc
    22441 acgtaatccg cagtgcggtt aattgaaatg gaaaaagtat gaagaactgg aaaacgctgc
    22501 ttctcggtat cgccatgatc gcgaatacca gtttcgctgc cccccaggta gtcgataaag
    22561 tcgcagccgt cgtcaataac ggcgtcgtgc tggaaagcga cgttgatgga ttaatgcagt
    22621 cggtaaaact gaacgctgcg caggcaaggc agcagcttcc tgatgacgcg acgctgcgcc
    22681 accaaatcat ggaacgtttg atcatggatc aaatcatcct gcagatgggg cagaaaatgg
    22741 gagtgaaaat ctccgatgag cagctggatc aggcgattgc taacatcgcg aaacagaaca
    22801 acatgacgct ggatcagatg cgcagccgtc tggcttacga tggtctgaac tacaacacct
    22861 atcgtaacca gatccgcaaa gagatgatta tctctgaagt gcgtaacaac gaggtgcgtc
    22921 gtcgcatcac catcctgccg caggaagtcg aatccctggc gcagcaggtg ggtaaccaaa
    22981 acgacgccag cactgagctg aacctgagcc acatcctgat cccgctgccg gaaaacccga
    23041 cttctgatca ggtgaacgaa gcggaaagcc aggcgcgcgc cattgtcgat caggcgcgta
    23101 acggcgctga tttcggtaag ctggcgattg ctcattctgc cgaccagcag gcgctgaacg
    23161 gcggccagat gggctggggc cgtattcagg agttgccggg tatcttcgcc caggcattaa
    23221 gcaccgcgaa gaaaggcgac attgttggcc cgattcgttc cggcgttggc ttccatattc
    23281 tgaaagttaa cgacctgcgc ggcgaaagca aaaatatctc ggtgaccgaa gttcatgctc
    23341 gccatattct gctgaaaccg tcgccgatca tgactgacga acaggcccgt gtgaaactgg
    23401 aacagattgc tgccgatatc aagagtggta aaacgacttt tgctgccgca gcgaaagagt
    23461 tctctcagga tccaggctct gctaaccagg gcggtgatct cggctgggct acaccagata
    23521 ttttcgatcc ggccttccgt gacgccctga cccgcctgaa caaaggtcaa atgagtgcac
    23581 cggttcactc ttcattcggc tggcatttaa tcgaactgct ggatacccgt aatgtcgata
    23641 aaaccgacgc tgcgcagaag gatcgtgcat accgcatgct gatgaaccgt aagttctcgg
    23701 aagaagcagc aagctggatg caggaacaac gcgccagcgc ctacgttaaa atcctgagca
    23761 actaatggtt aaaacccaac gtgttgtgat cactcccggc gagcccgccg ggattggccc
    23821 ggacttagtt gtccagcttg cacagcgtga gtggccggtc gaactggttg tttgtgccga
    23881 tgccactctc cttaccgacc gggcagcgat gctcggtttg ccgctcaccc tccgccctta
    23941 ttcccccaac tcccctgcac aaccgcaaac tgcgggcaca ttaacgctac ttcctgtcgc
    24001 gctacgcgaa tctgtcactg cggggcagtt agcgattgaa aatgggcatt acgtggtgga
    24061 gacgctggcg cgagcgtgtg atggctgtct gaacggtgaa tttgctgcgc tgatcacagg
    24121 ccccgtgcat aaaggcgtca ttaacgacgc aggtattccg tttactggtc ataccgagtt
    24181 tttcgaagag cgctcgcagg cgaaaaaagt ggtgatgatg ctggcgaccg aagaacttcg
    24241 cgtggcgctg gcaacgacgc atttaccgct gcgcgatatc gcagatgcta tcacccctgc
    24301 acttttgcac gaagtgattg ctattttgca tcacgatttg cgtacaaaat ttggtattgc
    24361 cgaaccgcgc attctggtct gcgggctgaa tccgcacgcg ggcgaaggcg gtcatatggg
    24421 tacggaagag atagacacca ttattccggt gctcaacgag ctgcgggcgc aggggatgaa
    24481 actcaacggg ccgctgcctg ccgataccct gtttcagccg aaatatctcg ataacgccga
    24541 cgccgtgctg gcgatgtacc acgatcaggg tcttcccgtg ctaaaatacc agggcttcgg
    24601 gcgcggtgtg aacattacgc tgggcctgcc ctttattcgc acatcagtgg accacggcac
    24661 cgcgcttgaa ctggcgggac gtggcgaagc cgatgtcggc agttttatta cggcgcttaa
    24721 tctcgccatc aaaatgattg ttaacaccca atgaataatc gagtccacca gggccactta
    24781 gcccgtaaac gcttcgggca aaactttctc aacgatcagt tcgtgatcga cagcattgtt
    24841 tctgccatta acccgcaaaa gggccaagca atggtcgaaa tcggccccgg tctggcggca
    24901 ttgaccgagc cggtcggcga acgtctggac cagctgacgg tcatcgaact tgaccgcgat
    24961 ctggcggcac gtctgcaaac gcatccattc ttaggcccga aactgacgat ttatcagcag
    25021 gatgcgatga cctttaactt tggcgaactg gccgagaaaa tgggtcagcc gctgcgtgtt
    25081 ttcggcaacc tgccttataa catctccacg ccgttgatgt tccatctgtt tagctatact
    25141 gatgccattg ccgacatgca ctttatgttg caaaaagagg tggtgaatcg tctggttgca
    25201 ggaccgaaca gcaaagcgta tggtcgatta agcgtcatgg cgcaatacta ttgcaacgtg
    25261 atcccagtgc tggaagtacc gccgtcagcc tttacaccac cacccaaagt ggattccgcc
    25321 gtcgtgcgcc tggttcctca tgcaacgatg cctcacccgg ttaaagatgt tcgcgtgttg
    25381 agccgcatca ccactgaagc ctttaaccag cgtcgtaaaa ccattcgtaa cagcctcggc
    25441 aacctgttta gcgtcgaggt gttaacggga atggggatcg acccggcgat gcgagcggaa
    25501 aatatctctg tcgcgcaata ttgccagatg gcgaactatc tggcggagaa cgcgcctttg
    25561 caggagagtt aacgatgatc aattcgcccc gagtgtgtat tcaggttcaa agcgtctaca
    25621 ttgaggcaca atcttcacct gataatgaac gttacgtttt tgcttatacc gtaaccatac
    25681 gcaatctggg gcgagcgcca gtgcagttgt tggggcgtta ctggctgatc accaatggca
    25741 atggccgtga aaccgaagtc cagggcgaag gagtggttgg cgtccagcca cttatcgcgc
    25801 ctggcgaaga gtaccagtac accagcggtg caatcattga aaccccgctg ggcaccatgc
    25861 agggtcacta cgaaatgatc gatgaaaatg gcgtcccttt cagcatcgac attcccgtat
    25921 tccgactcgc cgttcccaca ctcattcatt aaaagaatat ggcgacatac cttattggcg
    25981 acgttcatgg ttgttacgat gaactgatcg cattgctgca taaagtagaa tttacccctg
    26041 ggaaagatac cctctggctg acgggcgatc tggtcgcgcg cgggccgggt tcgctggatg
    26101 ttctgcgcta tgtgaaatcc ttaggcgaca gcgtacgtct ggtgctgggc aatcacgatc
    26161 tgcatctgct ggcggtattt gccgggatca gccgcaataa accgaaagat cgcctgacac
    26221 cgctgctgga agcgccggat gccgacgagc tgcttaactg gctgcgtcgc cagcctttgc
    26281 tgcaaatcga cgaagagaaa aagttggtga tggcccacgc agggatcacg ccgcagtggg
    26341 atctacagac cgccaaagag tgcgcgcgcg atgtagaagc ggtgctatcg agtgactcct
    26401 atcccttctt tctcgacgct atgtacggcg atatgccaaa taactggtca ccggaattgc
    26461 gggggctggg aagactgcgt tttatcacca acgcttttac ccgtatgcgt ttttgcttcc
    26521 cgaacggtca actggatatg tacagcaaag aatcgccgga agaggcccct gccccactga
    26581 aaccgtggtt tgcgattcct ggccctgtcg ctgaagaata cagcatcgcc tttggtcact
    26641 gggcatcgct ggagggcaaa ggtacgccgg aaggtatata cgcgctggat accggctgct
    26701 gctggggtgg tacattaacc tgcctgcgct gggaagataa acagtatttt gtccagccgt
    26761 cgaaccggca taaggatttg ggcgaagcgg cggcgtctta aacacagcct gatataggaa
    26821 ggccggataa gacgcgaccg gcgtcgcatc cggcactagc cgtaaattct atacaaaatt
    26881 accgccgctc cagaatctca aagcaatagc tgtgagagtt ctgcgcatca gcatcgtgga
    26941 attcgctgaa taccgattcc cagtcatccg gctcgtaatc cgggaaatgg gtgtcgcctt
    27001 ccacttctgc gtcgatatgc gtcagataca gtttctgcgc ttttggcagg aactgctcat
    27061 aaacgcgacc gccgccaatc accatgattt ctggtacgtc accacacgcc gcgatggctt
    27121 catccaccga cttcacccac gttacgcgat cgtccgtacc cggttgactg ctgaggataa
    27181 tatttttgcg tcctggcaac ggacgaccga ttgattccca agtatggcgg cccataatca
    27241 cgggtttatt taaggtgttg cgtttaaacc aggcgagatc ggcaggcagg ttccacggca
    27301 tggcgttttc catgccgata acgcgatcta ccgctaacgc cgcaatcaga ctgatcattg
    27361 agatttcccg ataaaaaaaa ttgtcgccac tatacgtaaa gcgtaaaccg tcgtcgactg
    27421 gtgcgaggat gatgttgagg aaaattttat attctgctgg cgagtccacg ctctctccct
    27481 ggactcgccg cattacaatg aaacaaaaac aaacagttag ctgtaaagtg tgatttacgt
    27541 cactctttat taggatgagg gtttcgtttc cggttcatcc gccatgttgc cggtatgttt
    27601 accttcttcg gttccctgcc agccatgtcg ttgaattaat gacagatgtt cgcggtcctc
    27661 ggtaatgatc tcacttaaca tcgcgctggt gcgtttatag accgccgcgc gggctttggt
    27721 gtcgttctca accattgcca tctcttccac catctgaata ttaaagcggc ggaacacatc
    27781 ggcacgttct cgcgcttcat acggccccag acctaaactt tccagtgcca gacgcccggt
    27841 tttcagcgca ccttcgaagg tttcacgctc cggcttttca acgcctgcct gacgcaaacg
    27901 aatgtagtgg tcgacatcgc gggcgcgggc aataatctgc aaatgcggga agtgttcttt
    27961 caccatctct gtcagttgca ggttggtttg cggatcgtcg atggcgttaa tcagcacttc
    28021 cgctttcgcc gctcccgcag attccagtaa atccatccgc gtggcatcgc cataaaacac
    28081 tttcatacca aatttacgca aggtttcgat atggtccgga tcgtgatcga gcaccaccat
    28141 tttcaccccg ctggagagca gtaaacgtcc ggtgatctgc ccaaaacgac cgaatccggc
    28201 aatgattacc cgcggctgtt cttcgtcgat ctcatcagct tcacgcgcct cttcagtaga
    28261 agattgctca aggcgattga ggatcaccag cagaatcggc gttgccgcca tcgacagcgc
    28321 caccgccagg gtcagcgatt tcgcccactc cggttccagc acattcgcca tctgcgccgt
    28381 gccaaatacc acaaaggcaa actcactgcc ctgccctaac aacaccgcaa accaacgacg
    28441 ctgtttattt ggcacttgca acggtcgggc aatcagccac agcatggcga ttttgatgat
    28501 gaggaaaccg agcagcaaaa tgacaatgcg caatgggttt tcaagcagcg tgccaaagtc
    28561 tatcgacatg ccaacaccga tgaaaaacag ccccaacagc aaacctttaa atggttcgat
    28621 atcgctctcc agcgcatgac ggtattccga gcttgccagc agtacgcccg ccagaaacgc
    28681 gcccatcgcc atcgacaagc cgacctcttc cagcagcaaa ccaaagccaa acacgaggaa
    28741 taacgccacg gcactaaaca cttcccgcaa gccagagcgg gctacaaaac gcagcgccgg
    28801 acgcgtgaca tagcgcccca gcaataccac cagcaccagc gcacccgcca cttttaacgc
    28861 cgagagagca aatgcgccca tcgtcgtcga ggcactgctc gttgccagta gcggaatcat
    28921 cgccaccagc gggatcgccg cgatatcctg gaacagcagc accgcaaagg cactgcgacc
    28981 catttgcgtc accatcagat tgcgttcatt catcgcctgc atggcaatcg ccgtagagga
    29041 gagcgccagc gtcatgccga tcaattccgc gacctgccag tgcagcccaa gtaacatgca
    29101 gaacagcccc agcaggccgc cgcaaatcac catctgcaat gcgccaccgc cgaacactgc
    29161 cgcacgcagc ttccacagcc tttgtggatc gagttcgagg ccgataataa acagcatcag
    29221 caccacccca atctcggcaa agtgcagaat agattcggca tcggtcacca gtcgcagccc
    29281 ccacgggcca ataatgcagc cggcgatcag gtagccaagt accgatccca gaccaagacg
    29341 taccgcaatg ggtacaatca gcgctgccga accgagataa atcagcgcct gaatcagcgt
    29401 atggctatcc atgatgggcc tcctgccatt ccagcagacg ttgcttatag tgacgcgcct
    29461 gcccttcgag ggtttcgtcg tcacaaataa aggtgcagtg catggcaaac ggtggcagcc
    29521 agttcagccc gcagtagatt gccgtcgcct gtagcggctg cgacagcaca tcaaagcccg
    29581 gatgcgcacc aatttcaaaa tggctttccc cgccgccggt cgtcaccgcc cacagcaaat
    29641 atttgccatg cagcgccttg ccgccgtgac cgtaagccca gccgtgcgac aaaactttat
    29701 cgatccaaag tttaagaagc ggaggaatgc tgtaccactg catcggatgc tgccagacga
    29761 tcagatcggc gcgagacagc gcctcctgct cggcggcaat atcgatattg aagtcaggat
    29821 agagttgata aagagagcga atttcgacgc cttccagcgt ccttgcctgt tcaagcatcc
    29881 gtttattcgc atgggaatga tgcggatacg gatgcgcata aattataaga atcataggta
    29941 gcccgcgtga ttacgccttt gttttcccac aaagtgtagt catatattca cgcggcttat
    30001 agtcaataat attgattcag aaaagagaga aatctgaaca atttacccca ttctctgccc
    30061 gcggatcgtc atattgctgg tttgcgccag tgacatccct cgagtctccg gggcaaacgc
    30121 tacggaaatc aacaagccaa acagcgagat acccgcctcc attagcatcg tgttactgat
    30181 accgtaatta ttgataaaga tcggcagtgc ccaggtcgaa acaatggtgc caatacggct
    30241 taaggacata atcacgccca cggcagaggc gcggatatct gtcgggaaga gttcattagg
    30301 atagagccac tgcaaattac ccggcccgcc agagaaaaag gcatacaccg caaaggccat
    30361 cactaccagc cagatcccca tatccgggat cagccccaaa accgccagcg ccagcgtcat
    30421 catggcaaag ctgccaatca acaatggacg ccgtccggca gtgtttaacc acagcatcgg
    30481 cggaatacag ccgagcataa agaacaggct aatcaccacg ttccccagcg ccgcgttttt
    30541 gccaacaccc aatcccaaca aaccaacgat ttgcgggcca aaggtgtaaa tggcgaacat
    30601 tgggatcacc tggcaggtcc agatggcggc aacaaacaga acaaaaggaa aatggcggcg
    30661 attaaacaga tcgcgaaaac gggtttgcta cggctgctct tcatcgaaag ccaccggttc
    30721 gccaaacagt tttatcatca tctcttcgca ctcttttact cgccctttgc gtaataacca
    30781 gcggggagat tcaggcagtt cgaatcgacc aatcaaaatc aacaaacagg ggatcgccgc
    30841 gctacccagc atccagcgcc agccgccttc cacatcataa agccagtagc cgaccagatc
    30901 ggcgcaggtc gcgccgacat accacatggc ggcgataaag ctgatggaaa atgcccgctg
    30961 acgggtactg gagaactcgg tgatcattga ggtggcgatg ggataatctg caccgatgac
    31021 aatgccgata agtacccgca tcaccaacag ttcgacgggg gatgaaacaa acatcgtcgc
    31081 caccgatatc acgccgatgg cgatgatatc aatgaggaac attttgcgcc gtcctacttt
    31141 atcggaaata tagccaaaca gcgatgtgcc aacgaacagc ccggcgagcg ttcccgcgcc
    31201 caacaagcca atccagtcag cgtccagttt tagcgccgga gtcagttgct ccagcgccac
    31261 gccaatcatt accagtacat aaccatccag aaacggacca ccgcttcccc acagcaaaat
    31321 gcggcggtga attgaggaga atttgagatc gtcaaagttt ctggacggtt gcatgggtta
    31381 ttcctgtttt tttgcaatga gagtcagtac ggcgttacgt atcaaaccaa catcagccgt
    31441 aacggaactc cacaccaaag gtgccgcgcg ggtattccca ctgttccagc gccgacccca
    31501 gccccagaat gcgacaggtg ccgcactcca gacatccggc gtaatcgaag cgcacactgc
    31561 cgtcatcctg cttcttgtac agacctgcgg ggcacgcttt caccagcagc tccagggcct
    31621 gtttatcggc atcggccttt acaacaatgt gcggatgctc ttcatcgaca ttgaatttat
    31681 tgacgcccag tttgacgtcc acattgacgg gagaagtcat aaaacggtca ctcctttcat
    31741 gccatccttg atcagattga tgaagcccac tttcttgccg tggcggagga ttttcttgcg
    31801 catcagttcc ggcgcactgc cgtcaatggt gaacaggtca cgcgccacgc ccaccgccag
    31861 ttccgggtag ccgctaaaca tgcgtgggtt atcaaggaag gccggtagtt tctggtacat
    31921 acgcatatcg cgcagcgggc cactctcaag atgctgacga tattccgcca gtttttgctt
    31981 actgaaatcg tcgcttttca tcgctgaaag cacggttttt gctgcggctt ccccggcggc
    32041 aatcgccaga tccataccgc gaatggtaaa accgaggttc atacacattc cggcggcatc
    32101 accggcaatc aatacgccgt caccaaccaa ctccggcagc atgttgatgc ctgcttccgg
    32161 cactacgtga gcggaatatt ccaccagctt gccgcccgcg atcagcggtg ccactgccgg
    32221 atgctgtttg aaatcttcca gcatttgcgg caccgatttt tttgcgtcat gcagatgatg
    32281 cagaccacaa accagcccca gcgacagggt attttcattg gtataaagga agccgccgcc
    32341 catcaggcca tcggtgggtg atcccgcaaa cagacaagcc gcgccctgat taccctgcaa
    32401 ctgaaaacgg tcttcgatta ccgacttcgg taactcgatc agttccttca cgccaaccgc
    32461 cacatccgtc ggtttaacgc gttttgccat ccccagcttt tcggcaagga tggagttcac
    32521 tccatcagca aggatcaccg ttttcgcttc aatcacatcg ccatcggctt ctacaccgac
    32581 gactttgcca tcgcgctgta cgaggttatc gacgcggatc ccggtaatta actgcgcgcc
    32641 cgcttcttcg gcctgctcca tcagccaggc atcaaattta ctgcgcaaaa cggagtaaga
    32701 acgctgggat ggcgaggttt cgtcaccgtt gcagtagtcc atagtcatcg ctgacttttc
    32761 cgtcataaac gcgagttttt catgggtgat caggcgttct accggggcgg attcggcgaa
    32821 accaggaata atgtgttcca ggctgtgggc atagagacgc ccgccggtga cgttcttggc
    32881 acctgcggaa ttgccacgct cgataactaa cacttgcgcc ccttcgcggg cgagcaccag
    32941 tgcggcaacc gaaccggcaa gcccagcacc gacgatgatg gcgtcaaaga tatcttcgga
    33001 cataagagct ccctgtcagc ggcagggcca aaatgcagcc ctgccagagt ggatcaacgc
    33061 gctaaagctg cggtcagcgc cggaaggatc ttcacggcgt caccaacaat gccgtaatcc
    33121 gcgtactgga agatcggcgc atttttatct ttattgatgg cgaaaatggt ttgcgatgca
    33181 ttagcaccaa ccatgtgctg gatctgcccg gagatcccca ccgccaggta cagttcaggt
    33241 ttcagcatca ggttggagat accgacatag cgttcgtgct ccatccattt ttcgttttcc
    33301 gccaccggac gagaacaggc caactccgca cctatcgcct tgcaaagctg ttctgccagc
    33361 gcaatgttct ctttgctgcc aataccgcga ccgacgctga ccaccagacg ggctttgtcg
    33421 agatcgacgc tgttgctctg gcgcgcctgg gttgccgtgc gggtaatcgc cacagccgga
    33481 gcctgccact ccacggtgtg cgtttcgcca gtgcgtgacg cgtctggctg agccgcatcg
    33541 aacgtgccgc tgctgatggt cagtaccgca tacggcgtgg caatgcgttc ttcgccaatc
    33601 gccagaccac cgtaaaccat atgtttcact gttgctttac cgtcctgtac gctgacggtg
    33661 ctggcatcgt tagacaccgc cgctttaagg cgataaccca gttttgccgc cagtaatttg
    33721 ccgcgacggg tgtttggcag cagcaccagg ccgtctgcgc cgtgctggcg aatagtgtca
    33781 gccatgacac cggcgtaatc ttcgatcatc cgatcgtccg gtttgccgtt taatttccag
    33841 acatgattag cgccgagctg gattgcctgt gcgccgtcgg catcattgag gacaaaggtg
    33901 ttgatttgat tagctaaagc ctgcgcaccg ttcatcagtt ccggcagacg agaaggggta
    33961 tcgctgaata cccagacttg agaaaacgtg ttcatagcat cccctgtaat taaatgactt
    34021 tgcgaagatt ttcagcaaat gcggcgatct gttcttcgcc gtcgccttca atcacgatgc
    34081 gctgacgttc gcgctgtttc ggcgcggcaa cctgttgttc tgaccaggct gcctctgcgt
    34141 taaaaccaat atccgccgcc gaccatacct ggacgggctt tttcgccgcg ccgagaatgg
    34201 ctttcatcga aggaatttgt ggggaattga tatcagtgga aacagcaaca accgcaggca
    34261 gcggaatgct taaggtttcg gtttcatctt ccagttcgcg ctcaacggtg agggtatctg
    34321 ccgtcaggga gataattttg ctgacgccgt taactgccgg aatattgagg atttcgccca
    34381 ccagcagacc aacctgctgg gcataaaggt cggaagaacc atcgccacag aggatcaaat
    34441 caaagcctgc tttctgggcg gctgcagcca gtgcgctcgc cgtttgttgc ggcagtgcct
    34501 gctcgaactg gtcatcaatc accacaatca gttcatccgg gccgcgcgat agtacatctt
    34561 tacgcccttt ggcgttggtc agggctttac cgcccacact taaggccgtc acctgcgcct
    34621 ctgctgcctg ttgctttagc tggcaagccg cttcaatagc gttcagatcg tattggctta
    34681 ttttggcatc ggctttgctg aagtctaatg aaccatcagc attattgacc gcaatatcct
    34741 gttcatcagg cacgcactta tagcaagtaa taatcttcat tgcatctcca gaaatcatga
    34801 aggaaacatc gttcatatcg gtggtgttaa ttacagatat cacgcccttt aatttggaaa
    34861 taaaacttta atcaccaata ttgaaaatgt cacacgcaga taaatccaac tttcaatatt
    34921 gttaagttcc tcaccaatat tgaaaacatg gcgtagaaaa aagaaatttt caatattgct
    34981 ttatggatca ccaatattga aagtcagatg ctattttttt actcacaaca caacataaca
    35041 aactgattaa taatcaaaat taacgagaaa agcgccctct attcacccga aaaaaaaatg
    35101 tgataacaat cacagaatgc agcttattga atacccatta tgagttagcc attaacgcgt
    35161 ccacgaggtt aataataatt atatcaaatg ttaacaaaaa taaaacaaac gggaacgcaa
    35221 aataaatatt cgttttcaca gtggaattaa ctcatgaaga atgaaaagag aaaaacggga
    35281 atagaaccga aggttttctt tccgccgtta ataatcgtcg gcatactttg ttggcttaca
    35341 gtcagagatc tggatgcagc gaatgtcgtt attaatgctg tattcagtta cgtcaccaat
    35401 gtatggggat gggcatttga atggtatatg gtggtgatgc ttttcggttg gttctggctg
    35461 gtgtttggcc cgtatgccaa aaagcgttta ggtaacgaac cacctgaatt tagtaccgcc
    35521 agttggatct ttatgatgtt cgcctcctgt acgtctgctg ccgtactgtt ctggggatcg
    35581 attgagatct actactacat ctccaccccg ccgtttggct tagaaccgaa ctcgacaggg
    35641 gcgaaagagt tggggctggc ttacagcttg ttccactggg gacctctgcc gtgggccact
    35701 tacagcttcc tttcagtcgc cttcgcttac ttcttctttg tccgcaaaat ggaagtgatt
    35761 cgccccagct cgacactggt gccgctggta ggtgaaaaac acgccaaagg gttgttcggc
    35821 actatcgtcg acaacttcta tctcgtcgcc ttgatcttcg cgatgggtac cagtctgggc
    35881 cttgccacgc cgctggtgac cgagtgtatg caatggttgt ttggcattcc gcataccctg
    35941 caactggacg ctatcatcat tacctgctgg attatcctca acgccatttg cgtcgcttgc
    36001 ggtctgcaaa aaggggtacg tatcgccagt gacgtgcgta gttacctgag cttcctgatg
    36061 ctgggttggg tgttcattgt cagcggtgcc agcttcatca tgaactactt caccgattcg
    36121 gtggggatgt tgctgatgta tctgccgcgc atgttgttct ataccgatcc catcgctaaa
    36181 ggcggcttcc cgcagggctg gaccgtgttc tactgggcat ggtgggtgat ttatgctatc
    36241 cagatgagta tcttcctcgc ccgcatctcc cgtggtcgta ctgtgcgtga actgtgcttc
    36301 ggcatggtgc tggggctgac agcgtcaacc tggatcctgt ggactgtact cggtagtaac
    36361 actctgctgt tgatagataa aaacatcatc aacattccaa atctgatcga acagtacggt
    36421 gtggcgcgcg ccatcattga aacctgggcc gctctgccac tcagcaccgc caccatgtgg
    36481 ggcttcttca tcctctgctt tattgccacc gttacactgg ttaacgcctg ctcttatacc
    36541 ctggcgatgt ccacttgccg cgaagtacgc gatggtgaag aaccacctct gctggtgcgt
    36601 atcggttggt caattctggt tggcattatc ggtattgttc tgctggcgct cggcggcctg
    36661 aaaccgattc aaaccgccat tatcgccgga ggatgcccgc tgttcttcgt caacattatg
    36721 gtgacgctct cctttattaa agacgcgaaa cagaactgga aagattaatt aacccccaaa
    36781 atatcaagag gttgaaagat ggattttaat ttaaatgatg agcaggaact gtttgtcgcc
    36841 ggtatccgcg aactgatggc cagcgaaaac tgggaggcct attttgccga gtgcgaccgt
    36901 gacagcgtct acccggaacg ttttgtcaaa gcactggcgg atatgggtat cgacagtctg
    36961 ctgatccctg aagagcacgg tggtctggac gcggggtttg ttactctcgc cgccgtgtgg
    37021 atggagctgg gacgtctggg ggcaccaacc tatgtgctgt accagttgcc gggcgggttc
    37081 aacaccttcc tgcgcgaagg cacacaagag cagatcgaca aaattatggc tttccgcggc
    37141 accggtaagc agatgtggaa ctcagcgatt accgaaccgg gcgcgggctc cgacgtgggt
    37201 agcctgaaaa cgacttatac ccgtagaaat ggtaagattt atcttaatgg tagtaagtgt
    37261 tttattacca gcagcgccta caccccgtac atcgtggtga tggcgcgcga cggggcttct
    37321 ccggacaaac ctgtctacac cgaatggttt gttgatatga gcaaaccggg catcaaagtg
    37381 accaaacttg aaaagctcgg tctgcgtatg gatagctgct gtgaaatcac ctttgacgac
    37441 gtggaactgg acgagaaaga catgttcggt cgggaaggta acggctttaa ccgcgtcaaa
    37501 gaagagttcg accatgaacg tttcctggta gccctcacca actacggtac ggcgatgtgc
    37561 gcttttgaag atgcggcgcg ctacgccaat cagcgcgtgc agtttggcga ggctattggt
    37621 cgtttccagt tgattcagga aaaattcgcc cacatggcga tcaaattaaa ctccatgaaa
    37681 aacatgctgt atgaagcagc gtggaaagca gacaacggca ccatcacctc tggcgatgca
    37741 gcggcaaata cttctgcgcc aatgcggcat ttgaagttgt ggatagcgca atgcaggtgc
    37801 tgggcggtgt cgggattgcg ggcaaccacc gcatcagccg cttctggcgt gatctgcgtg
    37861 tagaccgcgt ctccggggga tctgacgaaa tgcagatcct gacgctgggt cgcgcggtgc
    37921 tgaagcaata ccgctaagtc cgtgcgctgc ctgatgcgac gctaatgcgt cttatcaggc
    37981 ctacgggagc acgaatgtag gccggataaa gcgtttacgc cgcatccggc agtcatgcac
    38041 tacataaatt tttcaggagc taataatgga tcatctaccc atgccgaaat tcgggccgtt
    38101 ggccggattg cgcgttgtct tctccggtat cgaaatcgcc gggccgttcg ccgggcaaat
    38161 gttcgcagaa tggggtgcgg aagttatctg gatcgagaac gtcgcctagg ccgacaccat
    38221 tcgcgttcaa ccgaactacc cgcagctctc ccgccgcaat ttgcacgcgc tgtcgttaaa
    38281 tattttcaaa gatgaaggcc gcgaagcgtt tctgaaatta atggaaacca ccgatatctt
    38341 catcgaagcc agcaaaggtc cggcctttgc ccgtcgtggc attaccgatg aagtactgtg
    38401 gcagcacaac ccgaaactgg ttatcgctca cctgtccggt tttggtcagt acggcaccga
    38461 ggagtacacc aatcttccgg cctataacac catcgcccag gccttcagtg gttacctgat
    38521 tcagaacggt gatgttgacc agccaatgcc tgccttcccg tataccgccg attacttttc
    38581 tggcctgacc gccaccacgg cggcgctggc agcactgcat aaagtgcgtg aaaccggtaa
    38641 aggcgaaagt atcgacatcg ccatgtatga agtgatgctg cgtatgggcc agtacttcat
    38701 gatggattac ttcaacggcg gcgaaatgtg cccgcgcatg agcaaaggta aagatcccta
    38761 ctacgccggt tgcggtctgt ataaatgcgc cgacggctac atcgtgatgg aactggtggg
    38821 cattacccaa attgaagagt gctttaaaga tattgacctc gcacatctac tcggtacgcc
    38881 ggaaatcccg gaaggcactc agcttatcca ccgtatcgaa tgcccttacg gcccactggt
    38941 agaagaaaaa ctcgatgcct ggctggcggc acataccatc gcagaagtta aagaacgctt
    39001 tgccgaactg aatatcgcct gtgccaaagt actgaccgta ccggaactgg aaagcaatcc
    39061 acagtatgtt gcccgtgaat caatcactca gtggcaaacg atggatggtc gcacctgcaa
    39121 agggccgaac atcatgccga aattcaaaaa taaccccgga caaatctggc gcggaatgcc
    39181 ctcacatggc atggacacgg ctgccatttt gaaaaatatc ggctacagcg aaaacgacat
    39241 tcaggagttg gtcag
//
LOCUS       DAJTZL010000018        38341 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.018, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000018 DAJTZL010000000
VERSION     DAJTZL010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38341
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.018"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            63..1427
                     /locus_tag="OO544_000808"
     CDS             63..1427
                     /locus_tag="OO544_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6766480.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHMLLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSIVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPADIAANEALKVRLLETEGVKEVLIAEEEHSAYVKIDSKVTNRFEVEQA
                     IRQA"
     gene            complement(1555..2046)
                     /gene="yajQ"
                     /locus_tag="OO544_000809"
     CDS             complement(1555..2046)
                     /gene="yajQ"
                     /locus_tag="OO544_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HCR6766481.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            2214..3125
                     /gene="panE"
                     /locus_tag="OO544_000810"
     CDS             2214..3125
                     /gene="panE"
                     /locus_tag="OO544_000810"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HCR6766482.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            3088..3678
                     /gene="yajL"
                     /locus_tag="OO544_000811"
     CDS             3088..3678
                     /gene="yajL"
                     /locus_tag="OO544_000811"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HCR6766483.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKTHEVASQLVMAAGIYNYYE"
     gene            complement(3732..5180)
                     /gene="thiI"
                     /locus_tag="OO544_000812"
     CDS             complement(3732..5180)
                     /gene="thiI"
                     /locus_tag="OO544_000812"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HCR6766484.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDEKQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNR
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            5386..5628
                     /gene="xseB"
                     /locus_tag="OO544_000813"
     CDS             5386..5628
                     /gene="xseB"
                     /locus_tag="OO544_000813"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HCR6766485.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDTSLPPFTPDNE"
     gene            5628..6527
                     /gene="ispA"
                     /locus_tag="OO544_000814"
     CDS             5628..6527
                     /gene="ispA"
                     /locus_tag="OO544_000814"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HCR6766486.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDADMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            6552..8414
                     /gene="dxs"
                     /locus_tag="OO544_000815"
     CDS             6552..8414
                     /gene="dxs"
                     /locus_tag="OO544_000815"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HCR6766487.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVTAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPDAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            8469..9443
                     /gene="yajO"
                     /locus_tag="OO544_000816"
     CDS             8469..9443
                     /gene="yajO"
                     /locus_tag="OO544_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HCR6766488.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSINDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALKLQKQHGWAQFISMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHAVVGFK"
     gene            complement(9497..10015)
                     /gene="pgpA"
                     /locus_tag="OO544_000817"
     CDS             complement(9497..10015)
                     /gene="pgpA"
                     /locus_tag="OO544_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HCR6766489.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(9993..10970)
                     /gene="thiL"
                     /locus_tag="OO544_000818"
     CDS             complement(9993..10970)
                     /gene="thiL"
                     /locus_tag="OO544_000818"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HCR6766490.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFATP"
     gene            complement(11048..11467)
                     /gene="nusB"
                     /locus_tag="OO544_000819"
     CDS             complement(11048..11467)
                     /gene="nusB"
                     /locus_tag="OO544_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HCR6766491.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(11487..11957)
                     /gene="ribE"
                     /locus_tag="OO544_000820"
     CDS             complement(11487..11957)
                     /gene="ribE"
                     /locus_tag="OO544_000820"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HCR6766492.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(12046..13149)
                     /gene="ribD"
                     /locus_tag="OO544_000821"
     CDS             complement(12046..13149)
                     /gene="ribD"
                     /locus_tag="OO544_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HCR6766493.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGGVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSSEWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQAGLVDELIVYIAPKLLGSDARGLC
                     TLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(13153..13602)
                     /gene="nrdR"
                     /locus_tag="OO544_000822"
     CDS             complement(13153..13602)
                     /gene="nrdR"
                     /locus_tag="OO544_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HCR6766494.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            13753..14292
                     /locus_tag="OO544_000823"
     CDS             13753..14292
                     /locus_tag="OO544_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HCR6766495.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            14591..15475
                     /gene="tsx"
                     /locus_tag="OO544_000824"
     CDS             14591..15475
                     /gene="tsx"
                     /locus_tag="OO544_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HCR6766496.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYDAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(15652..15999)
                     /locus_tag="OO544_000825"
     CDS             complement(15652..15999)
                     /locus_tag="OO544_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease family protein"
                     /protein_id="HCR6766497.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(16128..17099)
                     /gene="secF"
                     /locus_tag="OO544_000826"
     CDS             complement(16128..17099)
                     /gene="secF"
                     /locus_tag="OO544_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HCR6766498.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(17110..18957)
                     /gene="secD"
                     /locus_tag="OO544_000827"
     CDS             complement(17110..18957)
                     /gene="secD"
                     /locus_tag="OO544_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HCR6766499.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(18985..19317)
                     /gene="yajC"
                     /locus_tag="OO544_000828"
     CDS             complement(18985..19317)
                     /gene="yajC"
                     /locus_tag="OO544_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HCR6766500.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(19340..20467)
                     /gene="tgt"
                     /locus_tag="OO544_000829"
     CDS             complement(19340..20467)
                     /gene="tgt"
                     /locus_tag="OO544_000829"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HCR6766501.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(20523..21593)
                     /gene="queA"
                     /locus_tag="OO544_000830"
     CDS             complement(20523..21593)
                     /gene="queA"
                     /locus_tag="OO544_000830"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HCR6766502.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            21686..22267
                     /gene="acpH"
                     /locus_tag="OO544_000831"
     CDS             21686..22267
                     /gene="acpH"
                     /locus_tag="OO544_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HCR6766503.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRSETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFV
                     CYAREQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQNVLNGMASRRPRLDALR
                     DSWYDLDAHYDALETRFWQFYPRMMAQASHKAL"
     gene            complement(22272..24089)
                     /gene="malZ"
                     /locus_tag="OO544_000832"
     CDS             complement(22272..24089)
                     /gene="malZ"
                     /locus_tag="OO544_000832"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HCR6766504.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVSMHKHRSQQQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLGLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWSMDGW
                     RLDVVHMLGEAGGARNNMQHVAGSTEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYQRMIALRKKSQALRHGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEACEV
                     VLPASPLLNAVQWQCKERHGQLTDGILALPAISATVWMN"
     gene            complement(24245..25618)
                     /gene="proY"
                     /locus_tag="OO544_000833"
     CDS             complement(24245..25618)
                     /gene="proY"
                     /locus_tag="OO544_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HCR6766505.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRCLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(25694..27013)
                     /gene="brnQ"
                     /locus_tag="OO544_000834"
     CDS             complement(25694..27013)
                     /gene="brnQ"
                     /locus_tag="OO544_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HCR6766506.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(27420..28715)
                     /gene="phoR"
                     /locus_tag="OO544_000835"
     CDS             complement(27420..28715)
                     /gene="phoR"
                     /locus_tag="OO544_000835"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HCR6766507.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEESGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(28773..29462)
                     /gene="phoB"
                     /locus_tag="OO544_000836"
     CDS             complement(28773..29462)
                     /gene="phoB"
                     /locus_tag="OO544_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HCR6766508.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDCAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            29652..30854
                     /gene="sbcD"
                     /locus_tag="OO544_000837"
     CDS             29652..30854
                     /gene="sbcD"
                     /locus_tag="OO544_000837"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HCR6766509.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETTQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            30851..33994
                     /gene="sbcC"
                     /locus_tag="OO544_000838"
     CDS             30851..33994
                     /gene="sbcC"
                     /locus_tag="OO544_000838"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HCR6766510.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVTLLTPEQV
                     QSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRQDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVASALAQHSEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTTSLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALTGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLLQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            34120..35304
                     /gene="araJ"
                     /locus_tag="OO544_000839"
     CDS             34120..35304
                     /gene="araJ"
                     /locus_tag="OO544_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HCR6766511.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGNLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISVFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKP
                     LG"
     gene            complement(35447..36355)
                     /gene="mak"
                     /locus_tag="OO544_000840"
     CDS             complement(35447..36355)
                     /gene="mak"
                     /locus_tag="OO544_000840"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HCR6766512.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVALNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            36480..37391
                     /gene="rdgC"
                     /locus_tag="OO544_000841"
     CDS             36480..37391
                     /gene="rdgC"
                     /locus_tag="OO544_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HCR6766513.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFALRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(37469..37924)
                     /locus_tag="OO544_000842"
     CDS             complement(37469..37924)
                     /locus_tag="OO544_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6766514.1"
                     /translation="MTQHPWSKLQREIYDLLTPTINLQIHCTRYLMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNAGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAADRRIGMRRLDQLRRKTHNIAALKIIARRSE"
     gene            complement(37921..38256)
                     /locus_tag="OO544_000843"
                     /pseudo
     CDS             complement(37921..38256)
                     /locus_tag="OO544_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
BASE COUNT         9474 a        10316 c         9728 g         8823 t
ORIGIN      
        1 actctgttta cccaacatgc gctccgcact acactatgtg ggcatttttt taaggggtgg
       61 taatgaacga ttataaaatg acgccaggtg agaggcgcgc gacctggggt ttagggaccg
      121 tattctcgtt gcgcatgctg ggcatgttta tggttctgcc ggttctgacc acgtacggca
      181 tggctctgca aggtgccagc gaagcattaa tcggtattgc cattggtatt tatggtctga
      241 ctcaggccgt ttttcagatt ccgtttggcc tgctttccga ccgcattggt cgcaaaccat
      301 taattgtcgg tgggctggcg gtgtttgccg ccggtagcgt tatcgctgcg ctttccgact
      361 ccatctgggg aattattctg ggccgggcgc tacaaggctc tggagcaatt gccgccgccg
      421 ttatggcgct gctttccgat ctcacgcgcg aacaaaaccg caccaaagcg atggcgttta
      481 tcggcgtgag ctttggcatt acctttgcca ttgcgatggt gcttggcccg atcatcactc
      541 acaaacttgg gctgcacgcg ctgttctgga tgatcgctat tctggcaaca accggcattg
      601 cgttgaccat ttgggttgtg cccaacagta gcactcacgt acttaatcgt gagtccggaa
      661 tggtgaaagg cagtttcagt aaagtgctgg cggaaccgcg gctgctgaaa ctcaactttg
      721 gcattatgtg tctgcacatg cttctgatgt ctacgtttgt tgccctgccc ggacagctag
      781 ctgatgcggg gttcccggcg gctgaacact ggaaggtcta tctggcgacg atgttaatcg
      841 cctttggctc gatcgtgcct ttcattatct acgctgaagt taagcgcaaa atgaagcaag
      901 tctttgtctt ctgcgtcggg ttgatcgtgg ttgcggaaat tgtgttgtgg aacgcacaaa
      961 cgcagttctg gcaactggtg gtcggcgtgc agcttttctt tgtggcgttt aatttgatgg
     1021 aagcccttct gccctcactt atcagtaaag agtcgccagc aggttacaaa ggtacggcga
     1081 tgggtgtcta ctccaccagc cagtttcttg gcgtggcgat tggcggttcg ctgggcggct
     1141 ggattgacgg catgtttgac ggtcaggggg tatttctcgc tggcgcaatg ctggccgcag
     1201 tgtggctggc agtcgccagt accatgaaag aaccgccgta tgtcagcagt ttgcgcattg
     1261 aaatcccggc ggacattgcc gcaaacgaag cgttaaaagt gcgtttgctg gaaactgaag
     1321 gcgtcaaaga agtgttgatt gcagaagaag agcattcagc ttatgtgaaa atcgacagca
     1381 aagtgacaaa tcgctttgag gttgaacagg caattcgtca ggcataaaaa acgcggcgaa
     1441 ttgcctgctg cgctacgctt accaggccta caaagttctt gagttacaaa ccgttgtagg
     1501 ccgaatcagg cgttcatgcc gcatctgaca tgaacaaaac gcacattgtc gcgattaatc
     1561 gcggaagttt ttgaactgga acggctggcc gagatcgcca ccacgtacca tcgccatgac
     1621 agcctgcaaa tcatcacgag atttgccagt cacacggatc tcatcgccct gaatttgcgc
     1681 ctgcactttc agtttgctgt ctttaatcat cttgacgatt ttcttctggg tcgcgctctc
     1741 aatgccctgt ttcagtttcg cttccacaaa ccaggtttta ccgctatgaa cgatattttc
     1801 cggtacatcc agtgaactgc cttcaatgcc gcgcttcagc agcttggcac gcagaatatc
     1861 cagcaactga ttgacctgga aatcggactc gctcaacact ttgatggttt tgctggcgtc
     1921 gttcagctca aatgaggctt caacattacg gaagtcaaaa cgggactcca cttcgcggct
     1981 cgcgttatcg accgcgttac gtgcttcctg aagatcaact tcagagacaa tatcgaaaga
     2041 tggcatcttt tcctctccct tcatttttga tgcgaagcat aatacccgca aagttaaata
     2101 accgacagtc ctgcaccagg acgtatccct taattgtgca tagtcacaac agcatgcaca
     2161 attaagggat agccttattg aacacccgga gtggttgcgg gtgaggagga acaatgaaaa
     2221 ttaccgtatt gggatgcggt gccttagggc aattatggct tacagcactt tgtaaacagg
     2281 gtcacgaagt tcagggctgg ctgcgcgtac cgcaacctta ttgtagtgtg aatctggttg
     2341 agactgatgg ttcgatattt aacgaatcgc tgaccgccaa cgatcccgat tttcttgcca
     2401 ccagcgatct gctcctggtg acgctgaaag catggcaggt ttccgatgcc gtcaaaagcc
     2461 tcgcgtccac actgcctgta accacgccaa tactgttaat tcacaacggc atgggcacca
     2521 tcgaagagtt gcaaaacatt cagcagccat tactgatggg caccaccacc catgccgccc
     2581 gccgcgacgg caatgtcatt attcatgtgg caaacggaat cacgcatatt ggtccggcac
     2641 ggcaacagga cggcgattac agctatctgg cggatatttt gcaaaccgtg ttgcctgacg
     2701 ttgcctggca taacaatatt cgcgccgagc tgtggcgcaa gctggccgtc aactgtgtga
     2761 ttaatccact gaccgccatc tggaattgcc cgaatggtga attacgtcat catccgcaag
     2821 aaattatgca gatatgcgaa gaagtcgcgg cagtgatcga gcgcgaaggg catcatactt
     2881 cagcagaaga tttgcgtgat tacgtgatgc aggtgattga tgccacagcg gaaaatatct
     2941 cgtcgatgtt gcaggatatc cgcgcgctgc gccacaccga aatcgactat atcaatggtt
     3001 ttctcttacg ccgcgcccgc gcgcatggga ttgccgtacc ggaaaacacc cgcctgtttg
     3061 aaatggtaaa aagaaaggag agtgaatatg agcgcatcgg cactggtttg cctcgcccct
     3121 ggtagtgaag agactgaagc cgtcaccact atcgatctgc tggttcgcgg cggtatcaaa
     3181 gtcaccactg ccagcgtcgc cagcgatggc aacctggcca ttacctgctc gcgcggcgtg
     3241 aagctgctgg cggatgcgcc gctggtcgag gtggctgatg gcgaatatga cgtgatcgta
     3301 ctgcctggtg gcattaaagg cgcagagtgt tttcgcgata gtaccctgct ggttgaaacc
     3361 gttaaacagt tccaccgttc cgggcgtatc gtcgcggcta tttgcgccgc gccagccacc
     3421 gtgctggtac cgcacgatat cttcccgatt ggcaatatga ccggcttccc gacgctgaaa
     3481 gacaaaattc ccgccgaaca atggcaggac aagcgcgtcg tctgggatgc acgggtaaaa
     3541 ttgctgacca gccaggggcc gggtacggct attgattttg gtctgaaaat tatcgacctg
     3601 ctggttgggc gcgagaaaac ccatgaagtg gcatcacaac tggtgatggc ggcagggatt
     3661 tataattatt acgagtagtg tcggatgcgg caaacgtcgc atctgaccag atgcgacgta
     3721 aaaaccacaa attatgggcg atacaccttc acattgttaa agccctgctc gcgcagatag
     3781 agcgcctgca gacggctcat caccccgcgc tcacaccaca gtagccaggt tctgttctgg
     3841 tcgagatcgc caaatttggt gctcagttta tagaacggca gagaaaccac gtcgatacct
     3901 tcaaccttca gcggcttatc ttcctgctca tcgacagaac ggatatcgag gatcacgtcg
     3961 ttcgggccaa agccattgac ggtttccact tccaccactt cctgctcggt ctgctgggcg
     4021 atttcgcgga tatcaacgtt attcgcttcc tcaaccactt tatcgagaat actgaagtcg
     4081 aacttctctt cttccgcttc aatcttcgat ttaaccgctt tcaccgtcgg gcttttggag
     4141 atcacgccac aatattctgg catcgtgcga gcaaagtctt cagtgccaat ctggcgggcc
     4201 aggttgatga tgtgctcttt gtcgtaagaa atcagcggac gcaggatcag cgtatcagag
     4261 acgttatcaa tcaggcgcag gttggtcagc gtctgactgg acacctggcc gagcgcttcg
     4321 ccggtgacca gcgcctgtac gccgtaacgt tcagccactt tagacgcggc acgcaccatc
     4381 atacgtttga ggataacgcc catctgaccg tcgtcgattt tctcgagaat ttccccgacg
     4441 accggttcga aattaatggc gacaaaacgc acgcggtggg agctgccaaa gcggttccac
     4501 agataatgcg ccacctgacg cacgccaatt tcatgcgctg cgccgccgag gttaaagaag
     4561 cagtaatgca cgcggcagcc gcgacgcatc aacatataac tggaaacacc ggagtcgaaa
     4621 ccaccggaaa tgagcgacag cacatcttcc tgggtgccaa tcgggaaacc gccaatacct
     4681 tcgtagcggc ctttaatcag cagaagacga tcgtcttcca cttccagatg gacagttaca
     4741 tccgggttag tcagcttcac gcgcgcggat tcaatatgct gatttaaacc gccgccgacg
     4801 taacgttcca catcaatcga gctaaaatca tgtttgccac ggcgcttcac gcgtacgcag
     4861 aaggttttgc cttccagctg atcgcgatat tgaaccaacg ctttctcgaa aatatcgtgc
     4921 atgtcggtaa acggcacgtc ttcgacttcg agaatatggt ggatacccgg aatacgggtc
     4981 agggcgtcgc gaatagccag acgctgtttt tcatcttttg cgcgaacttc gatgttatcc
     5041 cagtggcgga cgacagcgag cgtctcatca tagtgcttta aaacgttacg aatgttcccg
     5101 gtaaggattt ttataaagcg caagcgcaca gattggcttt tgatggtgat ttccgggaac
     5161 aatttaatga taaacttcat ggcggcaatg gttcgttggc aagccttaag cgacttgtat
     5221 agggaaaaat acagcagccc acacctgcgg ctgcatccag gcgcggaagt ataccactaa
     5281 catcgctttg ctgcgcacat caccttacca ttgcgcgtta tttgctattt gccctgagtc
     5341 cgttaccatg acggggcgaa aaatattgag agtcagacat tcattatgcc gaagaaaaat
     5401 gaggcgcccg ccagctttga aaaggcgctg agcgagctgg aacagattgt aacccgtctg
     5461 gaaagtggcg acctgccgct ggaagaggcg ctgaacgagt tcgaacgcgg cgtgcagctg
     5521 gcacgtcagg ggcaggccaa attacaacaa gccgaacagc gcgtacaaat tctgctgtct
     5581 gacaatgaag acacctctct accccctttt acaccggaca atgagtaatg gactttccgc
     5641 agcaacttga agcctgcgtt aagcaggcca accaggcgct gagccgtttt atcgccccac
     5701 tgccctttca gaacactccc gtggtcgaaa ccatgcagta tggcgcatta ttaggtggta
     5761 agcgcctgcg accttttctg gtttatgcca ccggtcatat gttcggcgtt agcacaaaca
     5821 cgctggacgc acccgctgcc gccgttgagt gtatccacgc ttactcatta attcatgatg
     5881 atttaccggc gatggatgat gacgatctgc gtcgcggttt gccgacctgc catgtgaagt
     5941 ttggcgaagc aaacgcgatt ctcgctggcg acgctttaca aacgctggcg ttctcgattt
     6001 taagcgatgc cgatatgccg gaagtgtcgg accgcgacag aatttcgatg atttcagaac
     6061 tggcaagcgc cagcggtatt gccggaatgt gcggtggtca ggcattagat ttagatgcgg
     6121 aaggcaaaca cgtacctctg gacgcactcg agcgtattca tcgtcataaa accggcgcat
     6181 tgattcgcgc cgccgttcgc cttggtgcat taagcgccgg agataaaggg cgtcgtgctc
     6241 tgccagtact cgacaagtac gcagagagca tcggccttgc cttccaggtt caggatgaca
     6301 tcctggatgt ggtgggagat actgcaacgt tgggaaaacg ccagggtgcc gaccagcaac
     6361 ttggtaaaag tacctaccct gcacttctgg gtcttgagca agcccggaag aaagcccggg
     6421 atctgatcga cgatgcccgc cagtcgctga aacaactggc tgaacaatca ctcgatacct
     6481 cggcactgga agcgctagcg gactacatca tccagcgtaa taaataaaca ataagtatta
     6541 ataggcccct gatgagtttt gatattgcca aatacccgac cctggcactg gtcgactcca
     6601 cccaggagtt acgactgttg ccgaaagaga gtttaccgaa actctgcgac gaactgcgcc
     6661 gctatttact cgacagcgtg agccgttcca gcgggcactt cgcctccggg ctgggcacgg
     6721 tcgaactgac cgtggcgctg cactatgtct acaacacccc gtttgaccaa ttgatttggg
     6781 atgtggggca tcaggcttat ccgcataaaa ttttgaccgg acgccgcgac aaaatcggca
     6841 ccatccgtca gaaaggcggt ctgcatccgt tcccgtggcg cggcgaaagc gaatatgacg
     6901 tattaagcgt cgggcattca tcaacctcca tcagtgccgg aattggtatt gcggttactg
     6961 ccgagaaaga aggcaaaaat cgccgcaccg tctgtgtcat tggcgatggc gcgattaccg
     7021 ctggcatggc gtttgaagcg atgaatcacg cgggcgatat ccgtcctgat atgctggtgg
     7081 tcctcaacga caatgaaatg tcgatttccg aaaatgtcgg cgcgctcaat aaccatctgg
     7141 cacagctgct ttccggtaag ctttactctt cgctgcgcga aggcgggaaa aaagttttct
     7201 ctggcgttcc gccaattaaa gagctgctca aacgtaccga agaacatatt aaaggcatgg
     7261 tagtgcctgg cacgttgttt gaagagctgg gctttaacta catcggcccg gttgacggtc
     7321 acgatgtgct ggggcttatc accacgctga agaacatgcg cgacctgaaa ggcccgcagt
     7381 tcctgcatat catgaccaaa aaaggtcgtg gttatgaacc ggcagaaaaa gaccccatca
     7441 ctttccacgc cgtgcctaaa tttgatccct ccagcggttg tttgccgaaa agtagcggcg
     7501 gtttgccgag ctattcaaaa atctttggcg actggttgtg cgaaacggca gcgaaagaca
     7561 acaagctgat ggcgattact ccggcgatgc gtgaaggttc cggcatggtc gagttttcac
     7621 gtaaattccc ggatcgttac ttcgacgtgg caatcgccga gcaacacgcg gtgacctttg
     7681 ccgccggtct ggcgattggt gggtacaaac ccattgtcgc gatttactcc actttcctgc
     7741 aacgcgccta tgatcaggtg ctgcatgacg tggcgattca aaagctcccg gtcctgttcg
     7801 ccatcgaccg cgcgggcatt gttggtgctg acggtcaaac ccatcagggc gcttttgacc
     7861 tctcttacct gcgctgcata ccggaaatgg tcattatgac cccgagcgat gaaaacgaat
     7921 gtcgccagat gctctatacc ggctatcact ataacgacgg cccgtccgcg gtgcgctacc
     7981 cgcgcggtaa cgcggttggc gtggaactga cgccgctgga aaaactgcca attggcaaag
     8041 gcattgtgaa gcgtcgtggc gagaaactgg cgatccttaa ctttggtacg ctgatgccag
     8101 acgcggcgaa agtcgctgaa tcgctgaacg ctacgctggt cgatatgcgt tttgtgaaac
     8161 cgcttgatga agcgttaatt ctggaaatgg ccgccagcca tgaagcgctg gtcaccgtag
     8221 aagaaaacgc cattatgggc ggcgcaggca gcggcgtgaa cgaagtgcta atggcccatc
     8281 gtaaaccagt acccgtgctg aacattggcc tgcctgactt ctttattcca caaggaactc
     8341 aggaagaaat gcgcgccgaa ctcggcctcg atgccgccgg tatggaagcc aaaatcaagg
     8401 cctggctggc ataatcccta ctccactcct gctatgctta agaaattatt catggcagga
     8461 gtggcagcat gcaatacaac cccttaggaa aaaccgacct tcgcgtttcc cgactttgcc
     8521 tcggctgtat gacctttggc gagccagatc gtggtaatca cgcatggaca ctaccggaag
     8581 agagtagccg tcccatcatt aaacgcgcgc tggaaggcgg cataaatttc tttgataccg
     8641 ccaatagcta ttccgatggc agcagcgaag agatcgttgg tcgcgcactg cgggatttcg
     8701 cccgtcgtga agacgtggtc gttgcgacga aagtgttcca tcgcgttggt gatttaccgg
     8761 aaggattatc ccgtgcacaa attttgcgct ctatcaacga cagcctgcgc cgtctcggca
     8821 tggattatgt cgatatcctg caaattcatc gctgggatta caacacgccg atcgaagaga
     8881 cgctggaagc cctgaacgac gtggtaaaag ccgggaaagc gcgttatatc ggcgcgtcat
     8941 ccatgcacgc ttcgcagttt gctcaggccc tgaagctaca aaaacagcac ggctgggcgc
     9001 agtttatcag tatgcaggat cactacaatc tgatttatcg cgaagaagag cgcgagatgc
     9061 tgcctctgtg ttatcaggag ggcgtggcgg tgattccgtg gagcccgctg gcgcgggggc
     9121 gactgacgcg tccgtgggga gaaactaccg cacgactggt gtctgatgag gtggggaaaa
     9181 atctctataa agaaagcgat gaaaatgacg cgcagatcgc agagcggtta acgggcgtca
     9241 gtgaagaact cggtgcaaca cgagcacaag ttgcgctggc ctggttattg agtaaaccgg
     9301 gcattgccgc accgattatc ggtacatcgc gggaagaaca gcttgatgag ctgctgaacg
     9361 cggtggatat cactttgaag ccagaacaga ttgccgaact ggaaacgccg tataaaccgc
     9421 atgcggtcgt aggatttaaa taagagtcca ggcctgatga gacgtgacaa gcgtcacatc
     9481 aggcatcggt gcacaactac gacagaatac ccagcggcca gtgatgaccg ataaagtaca
     9541 ggatgcctgc ggaaatcacc ccggcgacaa tatcgtcgat catgatcccc atgccgccat
     9601 gtacattgcg atcaaaccag cggatcggcc acggcttcca catatccaga atacggaaaa
     9661 tcacaaaccc ggcggcaacc cactgccagt cattggtcgg cagcgccatg agcgtgatcc
     9721 acataccaat aaattcgtcc cagacaatgc tgccgtgatc gtgcacgccc atatctttcg
     9781 ccgtttgatg acaaagatag acgccgatac agatccccaa catcaccacc agcgagtaga
     9841 gctgccaggg taaaaaggtc atcagatacc agaacggaat cgctgccagc gatcccatcg
     9901 tgccaggaac gattgggctt aatccactcc cgaatccgac agcaagcaga tgccacggat
     9961 tactcatctt caggcgactt ttcgcgacat ctttatggcg tggcaaaatg gtcatatcct
    10021 ttccagtcaa acgtgacagg ttcgccgtca cgaataaaac aaagcccttc gatatcggcg
    10081 gtcatttgcc cgatacaggt aaacggtacg ccaaggtgtc cgagagcaac atccagcgcg
    10141 ccacggttca gttccggcac cgtgaaacac aactcgtaat cttcaccgcc agagagcgcc
    10201 cagcgcagcg cctgttccgg ttcaacatgg cgagaaagcg catcagaaaa cggcagcaat
    10261 gccaggtcaa tacgtgcgcc gcagtcgctg gctttcacga tatgcccgag atcggaaatc
    10321 agaccgtcag agagatcgat agctgaattt gccagatcgc gcagtgcctg cccctgtaaa
    10381 atacgcggcg atggacggag atgacgtttg atcaagtagt cagcatcttt agcatcggca
    10441 acctgcaaac ggttttgcaa aatcgccagc ccggcggcgc tatcgcccgg tgtaccggtc
    10501 acatagatcc agtcacccgg tttcgcccca gagcgcgtta aggctcgtcc catcggaaca
    10561 aagccgtgga tacccaacgt cattgataat ggcccacgcg tggtatcgcc gccaatgagt
    10621 tgcatatcgt aataattgag aagatcaaac aaactgtcgc tgaaggactc aagccacgct
    10681 tcgtctacgt ccggtaaggt taatgccagc gtcagccagg ccggatcggc ccccatcgct
    10741 gccagatcgc ttaggttcac cgccagtgct ttataagcca gatcagcagg atcgatatca
    10801 gggaggaaat ggttacccgc caccagcgta tcagtgctga tcgccagggt ctgtttctcg
    10861 gggatattga gaagtgcgca atcgtcgccg atgcccagtt cgacatcaag acgagaactt
    10921 cttacacggt caaaataacg ggcaatcagg gagaactcgc cacatgccat acgttatgcc
    10981 tcagcaggta aaaaagaaaa ggccgacgat catggaacgg tcttccgtga atctaccggc
    11041 ctggatatca ctttttgtta gggcgaatca caggtgctgc tttatcgagt acgccgttga
    11101 cgaacttatg gctgtcttct gcgccgaacg atttcgccag ttcgatcgct tcgttaatgg
    11161 ccactttgta tggcacatcg ctacgtttag acagttcgta cagcgcaatg cgcagtactg
    11221 ctttttctac ctgtcccagt tcttccagca ggcgggacag gtatggcttc atcagtccgt
    11281 cgaggtatgc ggtattagtc gccaccccgg ccagcagctc acggaagtac aggacgtcaa
    11341 cgtcttttac atcctgttca gccaggaact ggtattcaac atcagcgatg tcgttctggg
    11401 acaactgcca ggagtagagc gcctggacgg cacactcacg agcgcggcga cgagcagcag
    11461 gtttcacgga tttcccctta ctaatttcag gccttgatgg ctttcaatac attaatcatt
    11521 tcaagcgcgg tcagtgcagc ttctgcacct ttgttgccag ctttggtgcc agcacgttcg
    11581 atcgcttgtt caatgctttc agtggtcaga accccaaaag caaccggaat ttcgctgtcc
    11641 tgggcaacat gcgccaggcc gttgcttgca ccaccagcga catattcaaa gtgggcagtg
    11701 ccaccacgaa taaccgtacc cagcgcaatc accgcgtcgt atttaccggt tttagccagt
    11761 gcacccgccg ccagcggcag ctcataggca ccaggcaccc aaacaacggt aatgttttca
    11821 tcttttacct gaccgatacg tttcagtgcg tcaattgcac cttccagcag gctgtcattg
    11881 ataaagttgt tgaaacgcgc gatggtgatg gcgacgcgag cgtccggggt agcaacgtta
    11941 gcttcaataa tgttcatttt cttccttcgg gttcgagtat ggccccgcag gggggcggat
    12001 tttagcataa tatttcgtgc gctgcttccc tttcgagccg ggagatcatg cacccactaa
    12061 atgcaggcaa acatccgggc ctacatgacg tatctctttg aatttaaatt ggggggcgtc
    12121 ggctaatttc tcaagccctg gcagcgtgca taatccgcgg gcgtcgctgc ctaatagttt
    12181 aggtgcgata tagacaatca gctcatcgac taaacccgcc tgcagcaatg cgccagcgag
    12241 cgttggcccc gcttccaccc agatgctgtt aatttgctgt ttacccagtt gcatcatcag
    12301 tacaaccaga tccagatgac ctttatgctc tggaatcagc aaggtacgca ccgtttccgg
    12361 ccactcacta gaatcttcct gcgtacgcgc gaaccaggtt tcgccgggct gctgcacaat
    12421 gcgatgttcc ggcgtcacgc gattttggct atcaatcaca atacgtactg gctggcggag
    12481 attttgttgc gggtagagcg cctgagtttg ttcatccagt tcagaccaac gcactgttaa
    12541 ggctggatca tccgccagca ccgtggcgct gctggttaaa atggcatgac tttgtgcgcg
    12601 ctgtcgttgt acgtcgcgcc gcgcctgagg cgaagtgatc cactggcttt cgccgctcgc
    12661 catcgccgtg cggccatcaa gcgatgcgcc aagttttaac tgaatataag gaaagccggt
    12721 gcgcatccgc ttgagaaagc ctttattcaa ttgctcggct tcactcatca tcagcccgtg
    12781 gctgacgtca atgccagcct gttgcagacg gtaaagtcca cgcccagcga cctgcgggtt
    12841 aggatcctgc atcgctgcga ccacgcgcgc tacgccagcg gcgatgagtg cgtcacagca
    12901 cggtggcgta cggccatgat ggctacaggg ttcgagtgtc acatacgccg ttgcgccttt
    12961 ggctttttca cccgccatac gcaacgcgtg tacttcggca tgtggttcac ccgctcgttg
    13021 gtggtaacct tcaccgacaa tttcgccatc tttgacaatg acgcccccga cattcgggtt
    13081 ggggtgggtg gtaaaacgtc ctcgttgcgc cagctttagc gcccgcgcca tgtaatactc
    13141 gtcctgcacg gcttagtcct ccaggcgcgc gatctcttcg ccaaattctt tgatatcttc
    13201 gaaactgcgg tagacagagg caaaacggat ataggcgact ttatcgagct ttttcaattg
    13261 ctccatcacc agattgccaa tcatcttgct cggtacttcg cgctcaccgg tggcgcgcag
    13321 ctgcgattta atatgattga ttgccatttc aacgtcatcg gaactcaccg gacgtttttc
    13381 cagcgcccgc agcattccgc tacgcaattt ctcttcatta aacggttcac gcacgtcgtt
    13441 gctttttaca acacgcggca taaccagctc cgccacttca aaggtggtga aacgttcatt
    13501 acacaccaga cactgccgac ggcggcgtac ggatgaaccc tcgcccacga gacgagagtc
    13561 aattacctta gtgtccacgg cgaaacagaa tgggcaatgc atacggcgtc ctgaccaggt
    13621 ggttaacgga aatctatttt accctgaact gacgatacaa caaaggcgca acgcttttga
    13681 gataaaggat cgatgctttc gctgctttta ccctctacca ttaggctaat tgcgacgaga
    13741 aagggacgac ctatgaatac taacgttttt cgactgctcc tgctgggaag cctgttcagc
    13801 cttagcgcct gtgtgcagca aagtgaagtg cgacagatga aacacagcgt cagcacgctg
    13861 aaccaggaga tgacgcaact caatcaagaa acagtcaaaa tcactcagca aaacaggctg
    13921 aatgcaaaat ccagcagtgg ggtttacctt ctgcctggag cgaaaacacc ggcaagactg
    13981 gaaagccaga tcggtacttt acgtatgtcg ctggtgaata ttacgcctga tacagatggc
    14041 accaccctta cactacgtat tcagggtgag tcgaatgatc ccttgcccgc attcagcgga
    14101 actgttgaat atgggcaaat tcagggaaca atagacaact ttcaggaaat caatgtgcag
    14161 aatcaattga ttaatgcccc tgccagcgtc ctcgccccca gcgatgttga tattccgtta
    14221 caattaaagg ggatttctgt ggatcagtta ggctttgtac gtatccacga cattcaacct
    14281 gttatgcagt aaacgtattt cgggacgatt ttgtgcgtcc cgcaacatct ttccccgtca
    14341 ttttgttact ctgcttacat cacctggatt gatagtaaaa gtttgcaaca agggcgaaag
    14401 tcagtacaat ccccgcccga atgtgtgtaa acgtgaacgc aatcgattac gtaaatgata
    14461 gaactgtgaa acgaaacata tttttgtgag caatgatttt tataataggc tcctctgtat
    14521 acgaaatatt tagaaacgca atttgcgcct ttttcactcc cgcaagggat tttcaaacag
    14581 tggcatacat atgaaaaaaa cattactggc agccggtgcg gtactggcgc tctcttcgtc
    14641 ttttactgtc aacgcagctg aaaacgacaa accgcagtat ctttccgact ggtggcacca
    14701 gagcgttaac gttgtcggaa gctatcacac ccgtttcgga ccgcagatcc gcaacgatac
    14761 ctaccttgag tacgaagcat tcgctaaaaa agactggttc gacttctatg gttatgcgga
    14821 tgcgccggta ttcttcggcg gtaactccga tgctaaaggt atctggaacc acggttctcc
    14881 gctgtttatg gaaatcgaac cacgtttctc catcgacaag ctgaccaata ctgaccttag
    14941 cttcggtccg ttcaaagagt ggtacttcgc gaacaactac atttacgaca tgggtcgtaa
    15001 taaagatggt cgccagagca cctggtacat gggtctgggt accgacatcg atactggtct
    15061 gccgatgagc ctgtccatga acgtctatgc gaaataccag tggcagaact atgacgcagc
    15121 gaacgaaaac gagtgggacg gttaccgttt caaaattaaa tactttgtgc cgattaccga
    15181 tctgtggggc ggtcagctga gctacatcgg cttcaccaac ttcgactggg gttccgattt
    15241 aggggatgac agcggtaacg caatcaacgg tattaagacc cgtactaata actctatcgc
    15301 ttccagccat attctggctc tgaactacga tcactggcac tactctgtcg tagctcgtta
    15361 ctggcacgac ggtggtcagt ggaacgacga tgcagaactg aacttcggca acggcaactt
    15421 caacgttcgc tctaccggct ggggtggtta cctggtagta ggttacaact tctgattatg
    15481 aaaatgccgg gatttattcc cggcatttct gattgttaac aacgtgtgct ttgttcatgc
    15541 cggatgcggc gtaaacgctt tgtccggccc acaaaagcac gtaaattcaa tatattgctg
    15601 aaataacgta ggcctgataa gcgtagcgca tcaggcaatt ttacgtttta atcacttctt
    15661 cttgttcatc atcgctttca ggtcagcgaa tgggttgtac ttcgcttcac cgacatcttt
    15721 ctgcgcatct tcccctgcga taacggtcgt accatactga tccgcttcgg tatatttcga
    15781 atgctcatga tcgtggcaat agagacaaag caattcccag ttactgccat cttccgggtt
    15841 attggtatgg tcgtgatcaa tgtggtgaac ggtaagttca cgcaggttgg aataaacaaa
    15901 ctcgcgggaa cagcgaccgc agacccacgg atagattttt aatgcttttt cgcgatagcc
    15961 gctttctaac cgcgcatagt tttttgggat gatagccatg aaaagttacc tggatgaaat
    16021 gtagggttac gcgcagtttc ccaagatgcg gggattttcg caatctccat acatcaaaaa
    16081 aaatcccgat cttctgaccg ggattttcaa catcaacggg aacttgatta cggcagaatt
    16141 gacggctgat ccgccccttc tttttccact ttctgctgca acatgtgttc gcgcttcata
    16201 cccagtttca gagccaacgc agatgctacg tagatggaag atgcagtgcc gatggaaaca
    16261 ccgataagca tggtcagcga gaagccttcc agtaccggac caccgaagag atacagcatc
    16321 aggataacca tcaaggtagt accggatgtg atcaaggtac ggtgcagcgt ctgggtcaag
    16381 gacacgttaa agatttcgta aggcgtaccg cgacggatct tgcggaagtt ttcacgaatt
    16441 cggtccgata ccacgatact gtcgttaagc gagtaaccga taaccgacat caacgatgcc
    16501 acaatggtca ggtcaatctc gatatggaat aacgacaaaa tacccagcgt aatgataacg
    16561 tcgtgcgcca gcgcaataac cacccctgcc gccagtcgcc actcaaagcg gaaacctacg
    16621 tacacgagga tagacagcag cgctgccatc aacgccatcg caccggtttg cgcaaggtct
    16681 gcccccacgc tcggaccgac gaactcaata cgcttcactg ctgcattctg attggtggat
    16741 tcgttaatca ccttcagaac ctggctgccc agcacctgac cgccggtttc gccttcagca
    16801 ggcggcatac ggaccatgat gtcgtggctg ctaccaaagt tttgcagcat tggctcttca
    16861 aaaccggctt tttgcaatgc atcacgcatt acgtcaattt cagccggttt ttcgagcgta
    16921 atttcgataa ccgtaccacc ggtgaaatcc agcccccagt taaagccgcg cacgcccata
    16981 ataacgatag cagcgattaa cagcagacca gagatgccga aagcccagta gtcccagcgc
    17041 ataaagtcat agactttacg gccgtggttt agttgttcaa cagtatattc ctgtgccaca
    17101 tcgcactcct cagattgaca gcttcttgac gcgcttgccg ccatatagca ggtttacgat
    17161 ggcacgcgta ccgacaatcg cggtaaacat cgacgtcgcc acaccgatac cggtagtaat
    17221 cgcgaaccct ttaattgccc cggtacccac tgcgtacagg atgatgactt taatcagcgt
    17281 ggtgatgttc gcatcgaaga tagaactgaa tgcgccacga taaccttcat caattgcctg
    17341 ttgaacagta cgtccgttgc tcaactcttc tttaatacgt tcgttgatca gtacgttcgc
    17401 atcgaccgct accgcaaggg ttaagacgat acccgcgata cctggcatac tcagtgttgc
    17461 gcctggcaac agcgacataa taccgacgat caagatcaag ttggcaatca gagcactggt
    17521 cgcaatcaga ccaaacttct tatagaagat gatcatgaac agaatagaaa ccagcagacc
    17581 ggcaaggcaa gcctccagcc cctgctcaat gttctgcata cccagggtcg ggccaattgt
    17641 acgttcttca acaatctgaa tcggcgcgat caacgcaccg gcacgcagca gcagtgacag
    17701 ctgacgggct tcgttcgggt tgttgatgcc ggtgatacgg aagctgttac ccagacgaga
    17761 ctggatgttg gcgatgttaa tcacctcttc ctgtttcacc agaaccgcac gaccatttgc
    17821 atctttctta ccgctgtctt tgtactccac aaacagggtt gccatcggtt tgccgatgtt
    17881 gtccttggtg aagttagaca taatgttgcc accagcgcta tcgagcgaga tgttaacctg
    17941 cggctggttg tattcgtcct ggctggaagt ggagtcggtg atatggtcac cggtcagaat
    18001 tacgcgtttg tacagcacaa ccggctgacc ttcgcgggtc tgtttcactt cagagtcgcc
    18061 cggtacgcga ccggatgccg cagcggcctg atcaacattg gtgtttacca gacggaattc
    18121 cagcgttgcc gtcgcgccca gaatctcttt cgcacgcgca gtgtcctgaa taccaggcag
    18181 ttcaacaacg atacggtcag caccctgacg ctgaaccacc ggctccgcca cgccaagttg
    18241 gtttacacgg ttacgcagga tattaatgtt ctgctgcacc gcatattcac gcgcttcact
    18301 cagacgagca tcgctcatta ccgcacgcag ctggttgctg ccctggctgc taatcaccag
    18361 gtccggatgg cgcttgctca gatacgcaat ggcttcatca cgagctttag catcgcggaa
    18421 agtgatgctc aggccgtagt tgttttcttt acgaacagtg gtatacggga tgcccttttc
    18481 gcgcaggtca ctgcgtaggc tatcgatatt ttgttcctgg agtttgccaa gcgcggtgtc
    18541 catatccact tccatcagga agtgaacgcc gccacgcagg tcaaggccga gcttcatcgg
    18601 ctcagcatga atagctgcca gccagcgcgg cgttgccggg gcaaggttaa gcgccacgac
    18661 gtatttgtca cccataacgc ccattaatgc ttcacgagcg cgcaactggg tgtcagtgga
    18721 gtcgaagcgc gcaagaatag cgccctcttc cagtgccaca gacttagcag ttattttttc
    18781 ttcttgtaac gttttctgga cctggatcag cgtttgctca ctggcggcga ctccgcgcgc
    18841 accagtgatc tgaacagccg gatcctcacc aaacaggttg ggaagcgcat acagcagacc
    18901 gatgacaatc accacgatca gcatgacgta cttccacaaa ggataacggt ttaacacggc
    18961 aattccctta gggaaaaatt ttaattacag cgccttcatg gtgcctttcg gcaggacggc
    19021 agctacgaag tcacgtttaa taactacttc agtggtgtca ttcagcgcga tagcaatgta
    19081 gccgttttcc gctactttgg ttacgcgacc aaccaggcca ccgttcgtca gaacttcatc
    19141 acctttggca atggagtcca tcagcttttt gtgttctttg gtgcgcttct gctgtggacg
    19201 caggatcatg aaatagaaaa tcagaccgaa taccaccagc atcaaaatca aagacatcgg
    19261 gctaccttgc gccggtgcac ccgttgccgc taccgcatca gaaataaaaa agctcattaa
    19321 atttccctca ttattaatat taatcaacgt tcaaaggtgg tacttctcgc ccctgacgct
    19381 ggtaaaaatc agttacgaag ctctctaatt taccctcttc aatagccttg cgtaaacccg
    19441 ccatcaaacg ctggtagtaa cgaaggttat gaatggtgtt gagtcgcgcg cctaatattt
    19501 cgttgcaacg gtcaagatga tgcaagtaag cgcgtgaata attgcgacag gtgtagcaat
    19561 cacactcagg atcaagtggg ccagtatcgc tcttatactt cgcattgcgg attttcacca
    19621 cgccatcggt cacgaacaaa tgaccatttc gggcgttgcg ggttggcatt acgcagtcaa
    19681 acatatcgat accacgacgt acgccttcaa ccaggtcttc tggtttacca acgcccatca
    19741 ggtaacgcgg tttgtctgcc ggaatttgcg ggcatacatg ctccagaatg cggtgcatat
    19801 ctgctttcgg ctcacccaca gccagaccgc cgacagcgta gccatcaaaa ccgatatcta
    19861 ccagaccttt aacagaaata tcacgtaaat cttcgtaaac gctgccctga atgataccaa
    19921 acagcgcatt tttgtttccg agactgtcaa aacgctcacg gctacgcttc gcccaacgca
    19981 gagacatctc catggagcgt tttgcgtaat cccagtcagc aggatacggc gtacactcat
    20041 caaagatcat gacgatatcc gaaccaagat cgtactgaat ctccattgat ttttcaggat
    20101 cgaggaaaat cggatcgccg ttgatcgggt tacggaagtg cacgccctgt tcggtgattt
    20161 tacgaatatc gccaaggctg aagacctgga agccgccgga gtcggtgagg atcggcccct
    20221 tccactgcat aaaatcgtgc agatcgccgt gcagtttcat gatttcctgg cccgggcgca
    20281 gccacaggtg gaaggtgttg ccgaggataa tttgcgcgcc agtggcttca acttcttccg
    20341 gcgtcatccc ttttacggtg ccgtaggtgc caacaggcat aaaacaaggc gtttccacta
    20401 cgccacgatc aaagaccagg cggccacggc gtgcgcgacc gtcggtggtg tccagttcaa
    20461 atttcatttt ttctccacta cgtcagaaaa acagtccaac gttttaaacc agcgccacgg
    20521 aattactccc cgacgcgctc attaattgcc tgcggattgt acgtgataaa catcgcatca
    20581 ccgtaactaa aaaagcgata tttctcttct accgctgctt tataggcgtt catggtgtgt
    20641 tgataaccgg caaaggccga aaccagcata atcagcgtcg attccggcag gtggaagttt
    20701 gtcaccagcg catcgaccac tttgtactgg aagcccggat agataaagat ttgggtatcg
    20761 tcaaagaacg gttcgataag atcgtttttc gccgcctgag ccgcgctttc cagcgaacgt
    20821 actgaggtgg tgccaaccgc aatcacccgg ttaccgcgcg ctttcgccgc cagtaccgcg
    20881 tctaccacat cctgcggcac ttcagcgtat tccgagtgca tgatgtgatc ttcaatggtg
    20941 tcgacgcgca ccggctggaa ggtgcccgca ccaacgtgca acgtcacaaa cgccatctcc
    21001 acgcctttgg cgcgtaattt ttccagcaaa ggttcgtcaa aatgcagacc tgcggtcggg
    21061 gctgcaaccg cgcccggttt ttcgctataa acggtttgat aaagttcgcg gtcagcgtct
    21121 tcgtccggac ggtcgatata cggcggcagc ggcatatggc cgatgctgtt gagaatatcc
    21181 agcaccgagc gttcatcatt aaattcgact tcaaacagtg cgccgtggcg cgcggtcatt
    21241 gttgcgttaa tactttcgtc atcgcccagc agcagttctg cgccaggttt tggcgctttc
    21301 gaggcgcgaa tatgcgcaag aatgcgtttg tcgtcgagca tccgttcaac cagcacttca
    21361 atcttgccgc cgctggcttt acgcccaaac aggcgcgccg ggatcacgcg ggtattatta
    21421 aaaaccagaa gatcgccggg gttgagctta tcaagtaaat cggtgaaagt accgtgcgtc
    21481 agcgcgcccg tcggcccgtc cagcgacagt aaacgacagc tactgcgttc aggcatggga
    21541 tagtgggcaa tcagggattc gggcaattca aaggagaaat cggtaacgcg catgacactg
    21601 actcagacta aaataagagg cgggtagtct agtgccgggg cgctctccct gcaaccttta
    21661 cccccttttg gatagaatat aatcgatgaa ttttttagct cacctgcatt tagcccatct
    21721 cgcggaaagc tcgctttccg gcaatttact ggctgatttc gtacgcggaa atcctgaaga
    21781 aagttttccg cccgacgtcg tggctggcat tcatatgcat cgacgtatcg acgtattaac
    21841 cgacaatctg ccggaagtcc gcgaagcacg ggaatggttt cgtagtgaaa ctcgccgcgt
    21901 tgcgcctatt acgctggatg tcatgtggga tcactttctt tcccgccact ggtcgcagct
    21961 gtcgccggac tttccgctac aggaatttgt ctgttatgcc cgcgagcaag tgatgacgat
    22021 tttgccggac tcaccgccac gttttatcaa tctgaacaat tacttgtggt cagagcagtg
    22081 gctggtgcgc tatcgcgata tggatttcat ccagaacgtg ttaaacggca tggcaagccg
    22141 ccgcccacgt ctggatgccc tgcgtgactc ctggtacgat ttagacgctc attatgacgc
    22201 cctcgaaacc cgcttctggc agttttatcc gcggatgatg gcgcaggcgt cacacaaggc
    22261 gttataaaac gttagttcat ccataccgta gccgaaatgg caggcaaagc cagaatcccg
    22321 tcagtcagtt gcccatgcct ttctttgcat tgccattgca cggcattgag caacggtgac
    22381 gcgggtagca cgacttcaca agcttcgcca cggttgattg ccaccagtac acgctgctga
    22441 ttcagcacgc ggacaaatac caccacgtta tcttccgcat acagcacctg acagccgcca
    22501 tgacgtagcg cctgactttt cttacgcagc gcaatcattc gctggtacag cgcgaataac
    22561 gccgtatcct gcttttccac ctgccagggg aacggtttac ggcaaaacgg atcgtttttg
    22621 ccatccagtc ctacttcatc accgtaataa atgcacggta caccaggcca ggtgaacagc
    22681 cagaccaccg ccagcggcag gcgcgcaata tcccgaccga gcagcgtttt aaatcgcgca
    22741 gtatcgtggc tgtcgagctg attaaacata cgtaattgtt gttgatgaga aagccctgcg
    22801 cggtaattat ccatccaggc catacaggtt tgggcatcaa tttgctgcgg atcgtaagag
    22861 atatcggtat tggcaagaaa tccccacaac gggaatgtga agccacgata gttcatggcg
    22921 gcatcttcca catcggcctg taaccattgc cgtgcatcgc caaaatgttc gccgacaata
    22981 tacgcttccg gctgggtttc tttcgccgct tcggtgctcc cagcaacgtg ctgcatatta
    23041 ttgcgcgccc cacccgcctc ccctagcata tgcaccacat ccagccgcca gccgtccata
    23101 ctccacggcg ctttcagcca atggcggaca atactgtctt ccccgcgata aatttcattc
    23161 accagacttt ccgactgata atccagcttc ggcaagctgg catagccaag ccagtcgagc
    23221 gccgtgccat catcactaaa cgagtaccag tcgcgccagg gcgattcagg gttgtgacaa
    23281 gctccccccg tgccacgatt gtgcctatca aaccaggcat gggaatcgcc actgtggtta
    23341 aacacgccat ccagccccag ccgcattccc agctgctgcg tattgtggcg taagcgcagc
    23401 aacgccccat caccgccaaa ctgcggatcg acatggcgat aatcctcggt atcgtattta
    23461 tgtacgctgg gagctttaaa caccggattg agatacagcg ccgtcacgcc aagctttttc
    23521 agatacggca gtttttcgct tatcccgtcc agatcgccgc catagaacgt tgatccgccc
    23581 gcctgcgccg tgaccggttc atcccagtca cgcaagatga tctcttgtcc ggctgcatga
    23641 tggtaataga catgatcctg ttcagcttca cgaggaagac tacgcgcaaa acgatcaggg
    23701 aagatctgat aaaaaatctg atccgcagcc cattgtgggc cgatatccgg tacatcgacg
    23761 gcaaactgct ccagtcgtgc cggcggcatt cggctgaagc cctgcggtgt aaaccaacgc
    23821 tggcgatcgt gccacagcag tttgaaactg taacgccgcc ggggttgtcc gctggagaga
    23881 tcaatcgccg cacgccatgc ggtgacgcca ggctgctgct gactgcgatg cttatgcatc
    23941 gatactgaca tttcttcgtt atcgtgttct gtacgcagca taatgcgctg cggtgggtct
    24001 tcgcccgtca gccacagtgt aatgagcagt tgatctttgc tttgtttaac aaatgggggc
    24061 accggcaggt gccatgcatt taacatcata attcccctgt gatgaaaatc tgatcacctt
    24121 gccatagcct aatgcaggat cactcatcat ctgaaggata tttgggggag gaggacgggg
    24181 gcggataact ttcggaatag ccggatcagg atttcctcct gaaccggctt acagaacgca
    24241 aggattactg gttttcagcc agctgacgat cgtggcgacg cttaaacatc cagccaatca
    24301 acagcacaac aatccacgcg aaaccgacat acagcgagat acgcgtatcc gggtgataac
    24361 caatcaaccc gataataaag agcaggaaaa tcagcccgcc gatggtcgtt gctaccccac
    24421 ccggcacttt aaatttcagc gccttaactt cttctggcgg caaacagcga cggaaggcaa
    24481 tttgcgacag caggatcata atccacaccc acaccgtggc gaaggttgcc agcgaagcga
    24541 tcaccaggaa gacgttttcc ggcatgatgt agttcagata caccgcaaac agcagcgcgg
    24601 tagtcatcac cagcaccgta acccacggaa taccgcgacg cgacgttttg ctgaaaattt
    24661 tcggcgcgct gccctgctct gccataccgt ggagcatacg gcctacgcca aagacgtcac
    24721 tgttaattgc cgatagcgaa gcggtcagca caacaaagtt aagaatgcta gcggcaaagg
    24781 taatgcccat atgctggaac gtcagcacga acgggctacc ggcagtgcca acctgattcc
    24841 acgggtagat agacataatg acgaacagcg taccgacgta gaacaccaga atacgcatcg
    24901 gcacggagtt aatcgcacgc ggtatcgatt tctcaggatc tttcgcttca ccggcggtaa
    24961 tcccgataat ttcgatccca ccgtaagcaa acatcaccat ttgcaacgac atcaccatcc
    25021 caagccagcc gttactgaag aagccgccgt tgctccacag attatgaata ccggtcggtt
    25081 gcccgccgtt gccaatcccc cagatgatga tgccgaaacc agcgacaatc atgatgatga
    25141 tggtggcgac tttaaagaac gagaaccaga attccagctc cccgaagacc ttcacgctca
    25201 tcaggtttac ggcgcaaatg atcagcacca cgctcagtac ccaaatccag tgcggcaccg
    25261 tcgggaacca gacacccata tagataccaa aagcggtcac atcggcgatg gcgacaataa
    25321 ggatttcaaa gcagtaggtc cagccggtaa tgtaacctgc cagcgggccg aggttttcct
    25381 gcgcataacg cgagaaagag ctggcggccg ggttatgtac cgacatttcc cccagcgcac
    25441 gcataatgat atacgccgcg ataccaccga taatataggc caacaacacg ctcggaccgg
    25501 ccattttgat ggcgtctgcc gaaccgtaaa acagcccggt accaattgct gaacccagtg
    25561 ccataaagcg tatgtggcgg gtacttagcc cacgctttag cttgttctta ctttccatca
    25621 attcctaacc catcaacaca ataaaaaacc acggggcatc gcagccccgt ggttaaaaca
    25681 aatgttcagt gatttagtga gcgctggagg tcacctgacg acctgccgcg cgatcccaga
    25741 taatggccag aaccaccatc accactgttg gcattaacca cgccagacct tgttcggcca
    25801 gcggtaaacg ctgcgcccag gacggtaaga tatcgctgaa tgcagatgct ttgatcccgt
    25861 cgagaatacc aaaaagcagg ctgataaaca tcggcggagc aatcacgcgg gacgaattat
    25921 gccaccatga gcgtgtaaaa cttaatacaa ccagtgcgat acacggcgga taaatagcgg
    25981 tcagcaccgg tacggagatc tggatcagct ggcttaagcc gaggttagaa accaccatcg
    26041 agaagccgcc gaggataaac accagcgtac gataagagag cggtacgtac tgggcaaaga
    26101 attctgcaca agcacaggtc aggccaactg ccgttaccaa gcaggcgatg aagattaacg
    26161 ccgccagcag gaagctaccg ccgccgccaa aggtgtgctg aacgtaagca tgcagaatag
    26221 cagcgccgtt tgcagactga tcgaccagcg acgcgctgtc tgaacccaga cggaacagcg
    26281 ccaggtacag cagagtcaga ccaacacccg ccatcaggcc agcccagacg gtataacggg
    26341 tcagcagacg cgcttcggta acgccacgag aacgcgccgc gttaacaata acgataccaa
    26401 acaccattgc gcccagcgta tccatggtca gatagccgtt aacgaagccg ttagaaaacg
    26461 cagcgttttg ataagcctca gtcgccgtgc tgatagaacc cgccggccag acaatcgcgg
    26521 caacagacag gatgaccagc gcgataattt tcagcggcgc aaggaagttg cccacggtat
    26581 ccagcagctt gcccggatag agcgaaacca gaataacgat agcgaaatag accaggctgt
    26641 agataaacag cggcagcgcg gaatcaccag tcagcggcgc aatccccact tcaaaggaaa
    26701 cggtagctgt acgcggcgta gcgaaaagcg gccccaccgc caggtaacaa accgttgcca
    26761 gcagcacgcc agcgacttta ccgattggcg tgctgaggct gtcaacaccg ccgccaactt
    26821 ttgccagcgc cactaccgtt aataccggca ggccaacggc agtaatgagg aagccgaatg
    26881 ccgcagtcca gacgtgttcg ccagcctgca agccgaccat tggagggaaa ataatgttac
    26941 ctgcgccgac gaacaacgca aatgtcataa agcccagagc gatgatatcg cgcgatctta
    27001 attgatgggt cataaatcat actgcctgtg gatgtggtgt tgtaacgtta aatttttgtg
    27061 ccaatccctg gggatgatac agcggtatat ttaaacaatt ttagcgggaa atactcccaa
    27121 ccaggcgcgg cgaagaatag tttttcgatt taccgcgtta atgcagtcag tatgacagca
    27181 cctgaagcgc aatttaaacg cttataacgt tttaaagcaa ggagggatct aaaaaccaga
    27241 ggtatatagc gataagaaat caccaaccag tataaaacac tatttccgtg aaaaagacat
    27301 ggctaatcat gcgaacaaaa aatcaccaac cgttgtaaaa tctaacgccc gtattttgac
    27361 tgcaaagaaa ataagccagc atcgactggc ttatggcaat aaaagatgac aaaggcggat
    27421 taatcgctgt ttttggcaat taaacgttcc gggataacaa aactgaaacg tgttcctttt
    27481 cctactgtac tctcaatatt caggcgactt tcgtgatgat tcacagcatg tttcacgatc
    27541 gctaacccta atccgctacc gccggtttgc cgggaacgcg ctttatcaac gcgataaaaa
    27601 cgctcggtca ggcgcggaat atgctccggt gcaatgcccg gtccgttatc ttcaacgcta
    27661 aattcggcac cgtgcggcac tcgctgccag cgtacggtga tatgcgtgcc ttctggagta
    27721 tgattcacgg cgttatagac caggttcgaa atcgcactgc gtagctgatc ttcgttgcca
    27781 gacactttga ggccgttatc tatctcaaag gtaaatgtct gttttttctg actcagagtc
    27841 tgagcctcgc gctcaacaac gcgcagcatc atcggcacat caaccttttc attgagcaat
    27901 tgcgtcggcg cggcttcaat tttcgacagc gttagcaact gcttcaccag cccttccatc
    27961 cgctgggtct gctcgcgcat ggtgtgcaac gctttttcgc gtaccgcgcc ttccagcggc
    28021 tgctcatcca tcatctccag gtaaccctgt aacacggtca atggcgtacg taactcatgg
    28081 ctcacgttgg caaaaaagtt acgccgcgcc ccttccagtt gatgcatttg cgtgacatca
    28141 cgcgccacca tcagcaactg tttgtgggta taaggcatga cgcgaatttc cagatgccgc
    28201 ccggtgttga gcaccagatt gagcgggcga gaaaaatcac gcgttttcag atattgcgta
    28261 aactccgggt aacgcagtag gttaaggatg ttctgcccgt tatcttccgg ccagcgcaaa
    28321 ccaagaattt gttgcgccag accgttacac cagaaaatac cgctctcttc cgtggtcagc
    28381 accaccgcgt cgggcagcga ctccgcgccg ctacgaaagc gtttaatcag attgcccagt
    28441 tcacggcggc gttttttatt tcgcagctgc atctggtgta agccgtatag tagcggttcc
    28501 cagctaccac gccccggtgg cggggtcata ctgcgatcca cccacagcca ccatgaaagg
    28561 cgcaataaat tccagaaatg ccagataagc agtcctgtta ccgatgccag caaaaaccag
    28621 ggcaggtaac caaaaaatgc acccaggatg aaagccggga ggcagcaaag tagcagctcc
    28681 agcaccagcc ttttccacga cagccgttcc agcacgtaag atactcccgt taagaaatca
    28741 taagccctgc tctgcgtccg atgagcaagg cgttaaaagc gggttgaaaa acgatatcct
    28801 gtaccgcgca cggtctgcac catgcggtca tgcccgccgg gctccagtgc tttacgcagg
    28861 cgacgaatgt ggacatcgac cgtgcggtct tccacataca cgttagttcc ccagacgtgg
    28921 tttaacagct gctcgcggct gtacacgcgc tcaggatgcg tcataaaaaa gtgcagcagt
    28981 ttaaattctg tcggccccat ctccagcggc tcttcgcccg ccatcactcg gtgagatgtc
    29041 gggtcgagac ttaatccctg catctcaatc acctcttcca ccgccattgg cgaaatacgg
    29101 cgcattaccg ctttgattcg cgccaccagc tccttcggcg aaaacggctt ggtgatatag
    29161 tcatccgcgc cggtttcaag gccgcgcacg cgatcttctt cttcccctct ggcggtcaac
    29221 atcaccactg gaatatcccg ggtcatcgac tcgcgcttaa ggtgtttgat gaactggata
    29281 ccggagccgc caggtaacat ccagtcaagg agaattaaat ccggccaggg ttcattcagt
    29341 tgattcacag cacagtcata atcttccgct tcgaccggct gaaagccatt ttgttcgagc
    29401 acgaagcaga ccatttcacg aattggagct tcatcttcta cgaccagaat acgtctcgcc
    29461 atgatttgcc ctgttgtaat aaataggttg cgatcattaa tgcgacgtca ttatgcgtca
    29521 gatttatgac agatttatga aaagctcgtc gcacatatct tcaggttatt gatttccgtg
    29581 gcgcagaaaa aagcaaatgg cacatctgtt tgggtataat cgcgcccatg ctttttcgcc
    29641 agggaaccgt tatgcgcatc cttcacacct cagactggca tctcggccag aacttctaca
    29701 gtaaaagccg cgaagctgaa catcaggctt ttcttgactg gctgctggag acaacacaaa
    29761 cccatcaggt ggatgcgatt attgttgccg gtgatgtttt cgataccggc tcgccgccca
    29821 gttacgcccg cacgttatac aaccgttttg ttgtcaattt acagcaaact ggctgtcatc
    29881 tggtggtact ggcaggaaac catgactcgg tcgccacgct gaatgaatcg cgcgatatca
    29941 tggcgttcct caatactacc gtggtcgcca gcgccggaca tgcgccgcaa atcttgcctc
    30001 gtcgcgacgg gacgccaggc gcagtgctgt gccccattcc gtttttacgt ccgcgtgaca
    30061 ttattaccag ccaggcgggg cttaacggta ttgaaaaaca gcagcattta ctggcagcga
    30121 ttaccgatta ttaccaacaa cactatgccg atgcctgcaa actgcgcggc gatcagcctc
    30181 tgcccatcat cgccacggga catttaacga ccgtgggggc cagtaaaagt gacgctgtgc
    30241 gtgacattta tattggcacg ctggacgcgt ttccggcaca aaactttcca ccagccgact
    30301 acatcgcgct cgggcatatt caccgcgcac agattattgg cggcatggaa catgttcgct
    30361 attgcggctc ccccattcca ctgagttttg atgaatgcgg taagagtaaa tatgtccatc
    30421 tggtgacatt ttcaaacggc aaattagaga gcgtggaaaa cctgaacgta ccggtaacgc
    30481 aacccatggc agtgctgaaa ggcgatctgg cgtcgattac cgcacagctg gaacagtggc
    30541 gcgatgtatc gcaggagcca ccagtctggc tggatatcga aatcactact gatgagtatc
    30601 tgcatgatat tcagcgcaaa atccaggcat taaccgaatc attgcctgtc gaagtattgc
    30661 tggtacgtcg gagtcgtgaa cagcgcgagc gtgtgttagc cagccaacag cgtgaaaccc
    30721 tcagcgaact cagcgtcgaa gaggtgttca atcgccgtct ggcactggaa gaactggatg
    30781 aatcgcagca gcaacgtctg cagcatcttt tcaccacgac gttgcatacc ctcgccggag
    30841 aacacgaagc atgaaaattc tcagcctgcg cctgaaaaac ctgaactcat taaaaggcga
    30901 atggaagatt gatttcaccc gcgagccgtt cgccagcaac gggctgtttg ctattaccgg
    30961 cccaacaggt gcggggaaaa ccaccctgct ggacgccatt tgtctggcgc tgtatcacga
    31021 aactccgcgt ctctctaacg tttcacaatc gcaaaatgat ctcatgaccc gcgataccgc
    31081 cgaatgtctg gcggaggtgg agtttgaagt gaaaggtgaa gcgtaccgtg cattctggag
    31141 ccagaatcgg gcgcgtaacc aacccgacgg taatttgcag gtgccacgcg tagagctggc
    31201 gcgctgcgcc gacggcaaaa ttctcgccga caaagtgaaa gataagctgg aactgacagc
    31261 gacgttaacc gggctggatt acgggcgctt cactcgttcg atgctgcttt cgcaggggca
    31321 atttgctgcc ttcctgaatg ccaaacccaa agaacgcgcg gaattgctcg aggagttaac
    31381 cggcactgaa atctacgggc aaatctcggc gatggttttt gagcagcaca aatcggcccg
    31441 cacagagctg gagaagctgc aagcgcaggc cagcggcgtc acgttgctca cgccggaaca
    31501 agtgcaatcg ctgacagcga gtttgcaggt acttactgac gaagaaaaac agttaattac
    31561 cgcgcagcag caagaacaac aatcgctaaa ctggttaacg cgtcaggacg aattgcagca
    31621 agaagccagc cgccgtcagc aggccttgca acaggcgtta gccgaagaag aaaaagcgca
    31681 acctcaactg gcggcgctta gtctggcaca accggcacga aatcttcgtc cacactggga
    31741 acgcatcgca gaacacagcg cggcgctggc gcatattcgc cagcagattg aagaagtaaa
    31801 tactcgctta cagagcacaa tggcgcttcg cgcgagcatt cgccaccacg cggcgaagca
    31861 gtcagcagaa ttacagcagc agcaacaaag cctgaatacc tggttacagg aacacgaccg
    31921 cttccgtcag tggaacaacg aactggcggg ttggcgtgcg cagttctccc aacaaaccag
    31981 cgatcgcgag catctgcggc aatggcagca acagttaacc catgctgagc aaaaacttaa
    32041 tgcgcttgcg gcgatcacgt tgacgttaac cgccgatgaa gttgctagcg ccctggcgca
    32101 acattccgag caacgcccac tgcgtcagcg cctggtcgcg ctgcatgggc agattgttcc
    32161 ccaacaaaaa cgtctggcgc agttaatggt cactatccag aatgtcactc tggaacagac
    32221 gcaacgtaat gtcgcactta acgaaatgcg ccagcgttat aaagaaaaga cgcagcaact
    32281 tgccgatgtg aaaaccattt gcgagcagga agcgcgcatc aaaacgctgg aagctcaacg
    32341 tgcacagtta caggcgggtc aaccttgtcc actttgtggt tccaccagcc acccggcggt
    32401 cgaggcgtat caggcgctgg agcctggtgt taatcagtct cgattactgg cgctggaaaa
    32461 cgaagttaaa aagctcggcg aagaaggtgc ggcgctgcgc gggcaactgg atgcattaac
    32521 gaagcagctt cagcgcgatg aaaacgaagc gcaaagcctc cgacaagatg agcaagcact
    32581 tactcaacaa tggcaagccg tcacgaccag cctcaatatc accttgcagc cacaggacga
    32641 tattcaaccg tggctggatg cacaagatga gcacgaacgc cagctgcggt tactcagcca
    32701 acgacatgaa ttacaagggc agattgccgc gcataatcag caaattatcc agtatcaaca
    32761 gcaaattgaa caacgccagc agcagctctt aacggcattg acgggttatg cactgacatt
    32821 gccacaggaa gatgaagaag agagctggtt ggcgacacgt cagcaagaag cgcagagctg
    32881 gcagcaacgc cagaacgaat taaccgcgct gcaaaaccgt attcagcagc tgacgccgat
    32941 tctggaaacg ttgccgcaaa gtgatgatct cccgcacagc gaagaaactg tggcgctgga
    33001 taactggcgg caggttcatg aacaatgtct cgcattacac agccagcagc agacgttaca
    33061 gcaacaggat gttctggcgg cgcaaagtct gcaaaaagcc caggcgcagt ttgacaccgc
    33121 gctacaggcc agcgtctttg acgatcagca ggcgttcctt gcggcgctaa tggatgaaca
    33181 aacactaacg cagctggaac agctcaagca gaatctggaa aaccagcgcc gtcaggcgca
    33241 aacgctggtc acacagacag cagaaacgct ggcacagcat caacaacacc gacctgacgg
    33301 gttggctctc actgtgacgg tggagcagat tcagcaagag ttagcgcaaa ctcaccaaaa
    33361 gttgcgtgaa aacaccacga gtcaaggcga gattcgccag cagctgaagc aggatgcaga
    33421 taaccgtcag caacaacaaa ccttactgca gcaaattgct caaatgacgc agcaggttga
    33481 ggactgggga tatctgaatt cgctaatagg ttccaaagag ggcgataaat tccgcaagtt
    33541 tgcccagggg ctgacgctgg ataatttagt ccatctcgct aatcagcaac ttacccggct
    33601 gcacgggcgc tatctgttac agcgcaaagc cagcgaggcg ctggaagtcg aggttgttga
    33661 tacctggcag gcagatgcag tacgcgatac ccgtaccctt tccggcggcg aaagtttcct
    33721 cgttagtctg gcgctggcgc tggcgctttc ggatctggtc agccataaaa cacgtattga
    33781 ctcgctgttc cttgatgaag gttttggcac gctggatagc gaaacgctgg ataccgccct
    33841 tgatgcgctg gatgccctga acgccagtgg caaaaccatc ggtgtgatta gccacgttga
    33901 agcgatgaaa gagcgtattc ctgtgcagat caaagtgaag aagatcaacg gcctgggcta
    33961 cagcaaactg gaaagtacgt ttgcagtgaa ataactattc agcaggataa tgaatacaga
    34021 ggggcgaatt atctcttggc cttgctggtc gttatcctgc aagctatcac tttattggct
    34081 acggtgattg gtagccgttc tggtggttgt gatggtggta tgaaaaaagt cattttatct
    34141 ttggctctgg gcacgtttgg tttggggatg gccgaatttg gcattatggg cgtgctcacg
    34201 gagctggcgc ataacgtagg aatttcgatt cctgctgccg ggcatatgat ctcgtattat
    34261 gcactggggg tggtggtcgg tgcgccaatc atcgcactct tttccagccg ctactcactc
    34321 aaacatatct tgttgtttct ggtggcgttg tgcgtcattg gcaacgccat gttcacgctc
    34381 tcttcgtctt acctgatgct tgccattggt cgcctggttt ccggctttcc gcatggcgca
    34441 ttttttggcg tcggcgcgat cgtgttatca aaaattatca aacccggaaa agtcaccgcc
    34501 gccgtggcgg ggatggtttc cgggatgaca gtcgccaatt tgctgggcat tccgctggga
    34561 acgtatttaa gtcaggaatt tagctggcgt tacacctttt tattgatcgc tgtttttaat
    34621 attgcggtga tggcatcggt ctatttttgg gtgccagata ttcgcgacga ggcgaaagga
    34681 aatctgcgcg aacaatttca ctttttgcgc agcccggccc cgtggttaat tttcgccgcc
    34741 acgatgtttg gcaacgcagg tgtctttgcc tggttcagct acgtaaagcc atacatgatg
    34801 tttatttccg ttttttcgga aacggcgatg acctttatta tgatgttagt tgggctaggg
    34861 atggtgctgg gaaatatgct aagtggcagg atttcaggac gttattcacc actgcgcatt
    34921 gcagcagtga ctgactttat cattgtactg gcactgctga tgctcttttt ctgcggcggc
    34981 atgaaaacaa cgtcgcttat ttttgctttt atttgttgcg cgggattatt tgccctttca
    35041 gcacctctgc aaatattgtt actgcaaaac gccaaaggcg gagagttatt aggtgccgca
    35101 ggtgggcaaa tagcgtttaa cctcggtagc gccgtcggcg catattgcgg aggtatgatg
    35161 ctgacgctgg ggctggcata taattacgtg gcgctgcctg ccgccctgct ttcgtttgct
    35221 gcgatgtcat cgttgctgct gtatggtcgc tataagcgcc agcaagcggc ggatagtccg
    35281 gtgctggcga aaccactggg gtagattact gtcacggtgc gcgttgctta ttgccggatg
    35341 cggcgtgaac gccttatccg ccctacgcgg ttctggcaca ttttgtaggc ctgataagac
    35401 gcggcaagcg tcgcatcagg catgatgcgc caattgccta cgttttttac tcttgtggcc
    35461 ataaccacgc agcgccgcgt acgccgctgg aatcaccgtg cttcgcctta cgcaccggcg
    35521 tttcacattc gccgccgaag acaaattgtt taatcaactg cccaaccgtt tgatataaac
    35581 ggtctacatt gctcatcccg ccccccagga caatcacatc cggatcgaga atattcacga
    35641 catgtgccag cgattttgcc agccgcagct cgtagcgacg caatgccagt tccgctaccg
    35701 gatcgctttc ttcaaccagg cggataattt cactgccttt cagcgcatgt ccgctcaaac
    35761 gacgataatc cgtcgcgaat cccgtgcccg aaataaaggt ttcaatacaa ccttgtttac
    35821 cgcaataaca agggacttcc tcgcgataac gcagctcgtc ttcatccatc cacggtagcg
    35881 gattgtgtcc ccactcgcct gccgtgccat tgccgccgat atgcgcccgc ccatttaatg
    35941 ccacgcccgc gccgcatccc gtgccgataa tcacggcaaa taccgtctgc gctcccgctg
    36001 ccgcgccatc tactgcttct gaaaccgcca gacagttggc atcatttgcc agccgcactt
    36061 cccgctgcaa cctcgcgctt aagtctttat cgaatggctg accgttgagc caggttgaat
    36121 tggcattctt caccacaccg gtgtagggcg aaatcgaacc tggaatgccc atacctaccg
    36181 ttccgcgctg ccctgtcgcc tgctccgcca tatcaaccaa cgtggcgatc gtttcaatag
    36241 tctgccggta atcatcacgc ggcgtgggca gacgatggcg gtacaactgc tcccctgcat
    36301 cgcccagtgc aatcacttca gttttggtgc cgcctaaatc gatacctata cgcactgtac
    36361 tctccttatt tttttcaata tcaatagcgt agagacggac aaccggattg gcaatgcaag
    36421 gccgccgaca attcgttatc atgcccgcta aatttaacga caaggccgtg gaaattatca
    36481 tgctgtggtt caaaaattta atggtttacc gtcttagccg cgagatttcg ctgcgtgcag
    36541 aagagatgga aaaacagcta gcctcgatgg catttacccc atgcggcagc caggacatgg
    36601 cgaagatggg ctgggttcct ccgatgggat cgcacagcga tgcgttaacg cacgttgcca
    36661 atggtcaaat tgttatctgc gcgcgcaaag aagaaaaaat cctcccgtct ccggtgatta
    36721 aacaggcgct ggaagcgaaa atcgccaaac tggaagcgga acaggcgcgt aagctgaaga
    36781 aaaccgaaaa agattcgctg aaagacgaag tgctgcactc tctgctgccg cgtgctttca
    36841 gccgttttag ccagacaatg atgtggatcg acacggttaa cggtttgatt atggtggact
    36901 gcgccagtgc caaaaaagcg gaagatacgc tggcattact gcgtaaaagc ctggggtcgt
    36961 taccggttgt accattgagc atggaaaacc cgattgaact gacgctgacc gaatgggttc
    37021 gctccggtag tgcggcacag ggcttccagc tgcttgatga ggccgagctg aaatcgttgc
    37081 tggaagatgg cggcgtgatc cgcgcgaaga aacaagatct gaccagcgaa gagatcacca
    37141 atcacattga agccggaaaa gtggtgacta aactggcgct cgactggcag cagcgcattc
    37201 agtttgtgat gtgcgacgat ggttcgctca agcgtcttaa gttctgcgac gagctgcgcg
    37261 atcaaaacga agatatcgac cgtgaagatt tcgccctgcg ttttgatgcc gatttcatcc
    37321 tgatgactgg tgaactggca gcgttaattc aaaacctgat tgaaggatta ggtggcgaag
    37381 cacaacgtta attgctgatt ttcctttaat gccggatgcg acgcctgccg cgtcttatcc
    37441 ggcgtacgaa gccacaccag gcatataatt attcgctacg gcgagcaata atttttagcg
    37501 cagcaatatt atgcgtttta cgccgtaact gatctaatcg cctcatccct attcgcctgt
    37561 cagcagcacg cagaatattc actaaccccc atttatcgct ggtaaattgt tttgttaaca
    37621 gctccgcttt tggggtatca atatattcac gcaataaatc agaaattgaa caaatatccg
    37681 tcaggtaagg ataccagcaa gtttcaccgt gaaaattacg cactccagcg ttacctgcaa
    37741 taaagtcttt cgggtaatcc cagatcacgt ttttatccaa cgtgatccaa taacgcggta
    37801 aatcggtact tccaccattt tggctgcgca tgagataacg ggtacagtga atttgcaggt
    37861 tgatcgttgg cgtcagtaag tcatagatct cccgctgtaa cttgctccat ggatgttgtg
    37921 tcattgtttt tcctcaagcc ggtgacgcgc ttcactgcga attttccgcg ttgccccggt
    37981 tttcactaaa tcacgtaact gcgaaagatc gggttgttcg acaaataaga gtaatgatag
    38041 atcctctttt aaccacgccg ttttcacatc ggcagccagc tgtggattat taatagccag
    38101 cgaccgatcc tgagactcag tcaatgtcgt taacaacgac gccggggtat gtgcgttgcg
    38161 taatgccgtt tggcgcaaat gatcattgcg actttgcagc aatgcacgcc accattcatc
    38221 atctgattta tcttttatcg tagccagttc cgcaaaaaat acctggagat cttcactgat
    38281 atttatatca accttgatga attatgaata tatcggaaat tgaaaaaatc gcagcaaagg
    38341 a
//
LOCUS       DAJTZL010000019        38142 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.019, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000019 DAJTZL010000000
VERSION     DAJTZL010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38142
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.019"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            19..93
                     /locus_tag="OO544_000844"
     tRNA            19..93
                     /locus_tag="OO544_000844"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:51..53,aa:Gln,seq:ctg)
     gene            complement(247..1422)
                     /gene="ubiF"
                     /locus_tag="OO544_000845"
     CDS             complement(247..1422)
                     /gene="ubiF"
                     /locus_tag="OO544_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HCR6766515.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPGSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQFQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            1568..2992
                     /gene="miaB"
                     /locus_tag="OO544_000846"
     CDS             1568..2992
                     /gene="miaB"
                     /locus_tag="OO544_000846"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HCR6766516.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDMAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGSTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            3145..4185
                     /locus_tag="OO544_000847"
     CDS             3145..4185
                     /locus_tag="OO544_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HCR6766517.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            4182..4649
                     /gene="ybeY"
                     /locus_tag="OO544_000848"
     CDS             4182..4649
                     /gene="ybeY"
                     /locus_tag="OO544_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HCR6766518.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            4739..5617
                     /gene="corC"
                     /locus_tag="OO544_000849"
     CDS             4739..5617
                     /gene="corC"
                     /locus_tag="OO544_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HCR6766519.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            5642..7180
                     /gene="lnt"
                     /locus_tag="OO544_000850"
     CDS             5642..7180
                     /gene="lnt"
                     /locus_tag="OO544_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HCR6766520.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDGDQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSVNGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEVQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     ARKR"
     gene            complement(7258..9135)
                     /locus_tag="OO544_000851"
     CDS             complement(7258..9135)
                     /locus_tag="OO544_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HCR6766521.1"
                     /translation="MSASSVKPLNVQLPAITLILFALCIGIFCYLAQWMSYEEVDQSA
                     LIHLGANVAPLTLSGEPWRLLSSIFLHSSVSHLLMNMFAFLVVGGVAEQILGKWRLLI
                     TWLFSGVFGGLISACYALRESEQIVISVGASGAILGIAGAAIATQFASGTGTYHKNQR
                     RVFPLLGMVALTLLYGARQTGIDNACHIGGLIAGGALGWLSARLSGQNRLVTEGGIIV
                     AGSLLLTGAIWLAQQQMDESVLQVRQSLREAFYPQEIEQERRQKKQQLAEERNALRET
                     LSAPVSREQASGDLLAEIADIHDMAISRDGNMLYAAIENTNSIVVFDLGQKKILHTFT
                     APIAKEKSVKHCGGCKDQGVRSLALSPDEKLIYATSFEANALSVINVATGEIIQSITT
                     GAHPDSLILSRDGTKAWVMNRTSNSVPAIDLVTYQHVADIPLEKYDGTGTSGKPGAWV
                     MALSPDERTLLVPGAGRGNIVRINTITHQKEDFPAGDARGTISAMRFRPENGEVIFAD
                     SQGISRISVGDQQASIMTQWCSRSVYSVEGISPDGQYLALVSYGLQGYVILLNINAGQ
                     IIGVYPASYVNHLRFSADGRKIFVMAKNGLIQMDRTRSLDPQAIIRHPQYGNVACIPE
                     P"
     gene            9590..10498
                     /gene="gltI"
                     /locus_tag="OO544_000852"
     CDS             9590..10498
                     /gene="gltI"
                     /locus_tag="OO544_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HCR6766522.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKDKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHSDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWEIVGKPQSQEAYGCMLRKDDPQFKKLMDDTTAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            10668..11408
                     /gene="gltJ"
                     /locus_tag="OO544_000853"
     CDS             10668..11408
                     /gene="gltJ"
                     /locus_tag="OO544_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HCR6766523.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAVIMLVMTLVERKVRLPGNMGGK"
     gene            11408..12082
                     /gene="gltK"
                     /locus_tag="OO544_000854"
     CDS             11408..12082
                     /gene="gltK"
                     /locus_tag="OO544_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HCR6766524.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            12082..12807
                     /gene="gltL"
                     /locus_tag="OO544_000855"
     CDS             12082..12807
                     /gene="gltL"
                     /locus_tag="OO544_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP binding
                     protein GltL"
                     /protein_id="HCR6766525.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            12925..13860
                     /gene="rihA"
                     /locus_tag="OO544_000856"
     CDS             12925..13860
                     /gene="rihA"
                     /locus_tag="OO544_000856"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HCR6766526.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYSQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            13952..15622
                     /gene="hscC"
                     /locus_tag="OO544_000857"
     CDS             13952..15622
                     /gene="hscC"
                     /locus_tag="OO544_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HCR6766527.1"
                     /translation="MDNAELAIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDEQRKHTRLAAELAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEVHASAGDNFLGGEDFTHMLVDDVLKRA
                     DVARTTLNESELAALYACVEAAKCSNQSPLHIRWQYQDETRECEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRSEDIEEVILTDICPYSLGVEVNRQGVSGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQDSITVNVYQGENHKVKNNILVESFDVPLKKTGAYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAKLEE
                     LWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRRASDYLAIEIP"
     gene            complement(15682..17133)
                     /gene="djlC"
                     /locus_tag="OO544_000858"
     CDS             complement(15682..17133)
                     /gene="djlC"
                     /locus_tag="OO544_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HCR6766528.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDATAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMAELIPVAKQWQAEEPESEDARYYQCAQRLYCGEGESLLADLCA
                     YWESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGKPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEQDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITQMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EDEKPQSITSIIKISLTVLMIGYALGKIAMLFS"
     gene            complement(17130..17837)
                     /locus_tag="OO544_000859"
     CDS             complement(17130..17837)
                     /locus_tag="OO544_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCR6766529.1"
                     /translation="MNKEEQYLLFALSAPMEILNQSCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWQRSSPEERKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRVGVLNNWLTEEESLWLQSRVYARAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNASLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
     gene            17939..18493
                     /locus_tag="OO544_000860"
     CDS             17939..18493
                     /locus_tag="OO544_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCR6766530.1"
                     /translation="MNKKSMYIFAIFIVAAITCISQPKKTTLRDKAMVNYAFDYLRSP
                     GSLPLTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLGWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGTGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLPAH"
     gene            complement(18503..19930)
                     /locus_tag="OO544_000861"
     CDS             complement(18503..19930)
                     /locus_tag="OO544_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HCR6766531.1"
                     /translation="MKNCWKILEIEETTDVDIIRRAYLALLLSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSAGRLDIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCD
                     YWREYPSTQADALILQWCRQHRVDYYPLVVMMIEARELVNDQGKQLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTGQETVEPEPQPQPMPVDDGGLGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(19927..20634)
                     /locus_tag="OO544_000862"
     CDS             complement(19927..20634)
                     /locus_tag="OO544_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCR6766532.1"
                     /translation="MDMGSQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRTIHRMIDNGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPALSWQP
                     LADYPTCPETLKDMSDL"
     gene            20798..21775
                     /locus_tag="OO544_000863"
     CDS             20798..21775
                     /locus_tag="OO544_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HCR6766533.1"
                     /translation="MVFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFASN
                     ALGWTLDRGEAPNYKEAVVWYQIAAESGMSYAQNNLGWMYRNGNGVAKDYALAFFWYK
                     QAALQGHSDAQNNLADLYEDGKGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEDLL"
     gene            complement(21845..22327)
                     /locus_tag="OO544_000864"
     CDS             complement(21845..22327)
                     /locus_tag="OO544_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HCR6766534.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            22562..25144
                     /gene="leuS"
                     /locus_tag="OO544_000865"
     CDS             22562..25144
                     /gene="leuS"
                     /locus_tag="OO544_000865"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HCR6766535.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNMLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVLVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            25159..25740
                     /gene="lptE"
                     /locus_tag="OO544_000866"
     CDS             25159..25740
                     /gene="lptE"
                     /locus_tag="OO544_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HCR6766536.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            25740..26771
                     /gene="holA"
                     /locus_tag="OO544_000867"
     CDS             25740..26771
                     /gene="holA"
                     /locus_tag="OO544_000867"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HCR6766537.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVTARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQTQLRQAVQLLTRTELTLKQDYGQSVWAELKGLSLLLCHK
                     PLADVFIDG"
     gene            26773..27414
                     /gene="nadD"
                     /locus_tag="OO544_000868"
     CDS             26773..27414
                     /gene="nadD"
                     /locus_tag="OO544_000868"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HCR6766538.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            27438..28049
                     /locus_tag="OO544_000869"
                     /pseudo
     CDS             27438..28049
                     /locus_tag="OO544_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415171.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
     gene            28309..28626
                     /gene="rsfS"
                     /locus_tag="OO544_000870"
                     /gene_synonym="rsfA"
     CDS             28309..28626
                     /gene="rsfS"
                     /locus_tag="OO544_000870"
                     /gene_synonym="rsfA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HCR6766539.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            28630..29097
                     /gene="rlmH"
                     /locus_tag="OO544_000871"
     CDS             28630..29097
                     /gene="rlmH"
                     /locus_tag="OO544_000871"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HCR6766540.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            29128..31029
                     /gene="mrdA"
                     /locus_tag="OO544_000872"
     CDS             29128..31029
                     /gene="mrdA"
                     /locus_tag="OO544_000872"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HCR6766541.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNAPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            31032..32144
                     /gene="mrdB"
                     /locus_tag="OO544_000873"
                     /gene_synonym="rodA"
     CDS             31032..32144
                     /gene="mrdB"
                     /locus_tag="OO544_000873"
                     /gene_synonym="rodA"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HCR6766542.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            32155..33243
                     /gene="rlpA"
                     /locus_tag="OO544_000874"
     CDS             32155..33243
                     /gene="rlpA"
                     /locus_tag="OO544_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HCR6766543.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDSVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            33382..34593
                     /gene="dacA"
                     /locus_tag="OO544_000875"
     CDS             33382..34593
                     /gene="dacA"
                     /locus_tag="OO544_000875"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HCR6766544.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            34703..34966
                     /locus_tag="OO544_000876"
     CDS             34703..34966
                     /locus_tag="OO544_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HCR6766545.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            35067..35708
                     /gene="lipB"
                     /locus_tag="OO544_000877"
     CDS             35067..35708
                     /gene="lipB"
                     /locus_tag="OO544_000877"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HCR6766546.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            35968..36921
                     /locus_tag="OO544_000878"
     CDS             35968..36921
                     /locus_tag="OO544_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HCR6766547.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHVLLL
                     PEGQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            37130..38095
                     /gene="lipA"
                     /locus_tag="OO544_000879"
     CDS             37130..38095
                     /gene="lipA"
                     /locus_tag="OO544_000879"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HCR6766548.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
BASE COUNT         9559 a         9734 c         9572 g         9277 t
ORIGIN      
        1 catcgtaagt gcaccagttg gggtatcgcc aagcggtaag gcaccggatt ctgattccgg
       61 cattccgagg ttcgaatcct cgtaccccag ccacattaaa aaagctcgct tcggcgagct
      121 ttttgctttt ctgcgttcat tcaatgtcga atgcgatgtt gacacgtctt atccttcaat
      181 gtcggatgcg acgctgccgc gtcttatccg acctacggtt ggcacgcatc cggcaatgtt
      241 gtaaggctac aaccctaacg catatttcag cgcctgacgt ttcaacacgc cagcacgctc
      301 tgccgccatt aacccgagat tacgcacaaa acgcagcggc ggcagattat tactgaatcc
      361 ggcataaaac agatccatac cactttgcat aatgaagtta tccgccatgc gccgcatctg
      421 gtaacgcttg aggacaggat aactggccca cgcttcaccg tagctgcggg cattcaccag
      481 aacatcaatc agggcatcga catcacgata accaagattc accccctgcc ccgccagcgg
      541 atggatggta tgcgcggcat cgcccaccag cgcaagcccc ggctgcacat actgcaacgc
      601 atgtcggcgc gtcagcggaa acgcaccagc ggcaagcggt gtaacgtaac ccagacgcga
      661 cgggaaatgc ttcgcgattt ccgcctggaa ttgcgccata ttcatattct gcaactggcg
      721 aatacgcgcc ggagagtcat accacaccag cgatgcccag ttatcaaaca acggcagaaa
      781 cgcacgcggt ccgtccgggg taaattgctg ccaggtgctg tcgccgggat cgttctcaca
      841 ctgtacgcta atcaacatgc acgactgcgc atactgccag gcatgaacgc caattcccgc
      901 catctgccgc acctgagaat ttgcgccgtc ggcaccaatc accagtttcg cgcggatcgt
      961 ttcaccgccc ttcagttcca gctcctgaag atcattatct cgatgtaatg caatcagcga
     1021 gcctggcaca cgtaacgtta ctttcggatg cgcttccagc gcctgccaca gcgcctgttg
     1081 caggacggtg ttttccacca tatagccaag caacggtagc ttaagttcag cggcgtcaaa
     1141 caccacatgc gccgtttccc actcccacgt ttccagtctg cggtaaggat ggcaacgcat
     1201 agcctgtact gcatcccaga cccctaaccc tttaagcaat gataccgatg cggcgctgat
     1261 cgccgagatc cgcacgtccg gttggctatc agcgacaaac ggtgcaggtt ctgcgtgctc
     1321 gatcaccgtt accgaaaatc cgtgctgtgc cagccccagc gccagcgcgc cgccgaccat
     1381 tcctccgccg acaatggcaa tttccgttgg ttgatttgtc atggtcaatc atcctgttag
     1441 caatatgctt taagtttaca ggatttttac ccccaagggc tgataacgct catactggtc
     1501 acaacggcag caaagcatta cactatacgc cctgcattcc tggctactat ttcgcaagag
     1561 caagtcgatg accaaaaaac tccatattaa aacctggggc tgtcagatga acgagtacga
     1621 ttcatcgaag atggccgatc tgctggatgc cacccacggc tatcaactga ccgacatggc
     1681 ggaagaagcg gatgtgctgc tgctgaacac ctgctcaatc cgcgagaagg ctcaggaaaa
     1741 agtcttccat cagttgggtc gctggaaact gttaaaagag aagaatccag acctgattat
     1801 cggcgtcggt ggctgcgtgg catcgcaaga aggcgagcac attcgccagc gcgcccacta
     1861 tgtcgatatt atttttgggc cgcaaacgct gcaccgtctg ccggagatga tcaactccgt
     1921 gcgcggcgac cgcagcccgg ttgtagatat cagcttcccg gaaatcgaga agtttgaccg
     1981 tctaccggaa ccgcgcgccg aagggccgac cgcgtttgtc tccatcatgg aaggctgcaa
     2041 taaatattgc acctactgcg tggtgcctta cacccgtggt gaagaggtaa gccgtccgtc
     2101 tgacgatatt ctgtttgaga ttgcccagct tgcggctcag ggcgtgcgtg aagtcaacct
     2161 gctcggtcag aacgtgaacg cctggcgtgg tgaaaactac gacggctcca ccggatcgtt
     2221 tgccgatctg ctgcgtctgg ttgctgcgat cgacgggatc gatcgtattc gctttaccac
     2281 cagccatccg atcgaattca ccgacgatat catcgaagtg tatcgcgaca cgccggagct
     2341 ggtgagcttc ctgcatctgc cagtacaaag cggttccgat cgcattctga acctgatggg
     2401 ccgtactcac acggcgctgg agtacaaagc gatcatccgt aaactgcgtg cggcgcgtcc
     2461 ggatattcag atcagttctg acttcattgt tggcttccct ggcgaaacca ccgaagactt
     2521 cgagaaaacg atgaagctga ttgccgacgt caatttcgac atgagctaca gctttatctt
     2581 ctctgcacgt ccgggtacac cagccgccga tatggttgat gatgttccgg aagaagagaa
     2641 gaagcaacgt ctgtatattc tgcaagaacg tatcaatcag caagcgatgg catggagccg
     2701 ccggatgctc ggcaccaccc agcgtattct ggtagaaggg acttcgcgta agagcatcat
     2761 ggagctttcc gggcgtaccg aaaataaccg cgtggtcaac ttcgagggca caccggatat
     2821 gatcggtaaa ttcgtcgatg tagaaattac cgacgtctac ccgaactctc tgcgcggtaa
     2881 agtggtgcgt actgaagatg aaatgggtct gcgcgtggca gaaacaccgg aatcggtgat
     2941 tgcccgtacc cgcaaagaaa acgaccttgg cgtgggttat tatcagccgt aattctcagg
     3001 cccgccgttc cggtgggcct tgttttttct ctctttatcc ccatctttat cgcaatgctt
     3061 gcgccttttt cctgtggcgt gaataatttc cttataggcc ggtgaatgtt cagcttccgg
     3121 cggcatacag ataagaggaa cggtttgaac atagacactc gcgaaatcac cctggagcca
     3181 gcagacaacg cgcgtctgtt gagcctgtgc ggcccgtttg atgacaacat caagcagctc
     3241 gaacgccgtc tcggcatcga gatcaatcgc cgcgacaacc actttaaact gaccggccgt
     3301 ccgatttgcg tcaccgctgc ggcagacatt ctgcgtagcc tgtatgtcga tacagccccg
     3361 atgcgcggtc agattcagga tatcgaaccg gaacagatcc accttgcgat taaagaagcg
     3421 cgagtactgg agcaaagtgc ggagagcgtg ccggagtacg gcaaagcggt caatatcaaa
     3481 accaaacgcg gcgtaattaa gccgcgcacg ccaaaccagg cgcagtacat cgccaatatt
     3541 ctcgaccatg acattacctt cggcgttggc ccggcgggta cgggtaaaac ctacctggca
     3601 gtggctgcgg cagttgatgc cctggagcgt caggagattc gccgtattct gctgactcgt
     3661 ccggcggtcg aagccggtga gaaactgggc ttcctgcctg gcgatttaag ccagaaagta
     3721 gacccgtatc tgcgcccact gtacgacgcg ctgtttgaaa tgctgggctt tgagaaagtt
     3781 gagaaactga ttgagcgcaa cgttattgaa gtcgcgccgc tggcctatat gcgtggtcgt
     3841 acgctgaacg acgcgtttat cattctcgat gagagccaga acaccaccat cgaacagatg
     3901 aagatgttcc tgacccgtat cggttttaac tcaaaagcgg ttatcaccgg cgacgtcaca
     3961 cagatcgacc tgccgcgtaa tactaaatca ggcttacgtc acgccatcga agtgctggcc
     4021 gatgtcgaag agatcagctt taacttcttc cacagcgaag acgtggttcg tcaccctgtg
     4081 gtggcgcgta tcgttaacgc ctatgaagcc tgggaagaag ccgaacaaaa acgaaaagcg
     4141 gcgctggcgg cagaacgcaa gcgcgaagaa caggaacaaa aatgagtcag gtgatcctcg
     4201 atttacaact ggcatgtgaa gataattccg ggttaccgga agagagccag tttcaaacat
     4261 ggctgaatgc ggtgatcccg cagtttcagg aagaatcgga agtgactatt cgcgtggtcg
     4321 ataccgccga aagccacagt ctgaatctga cctatcgcgg taaggataag ccgaccaacg
     4381 tgctctcctt cccgtttgaa gtgccgcctg gcatggaaat gtcgctactg ggcgatctgg
     4441 ttatctgccg tcaggtggtt gagaaggaag ctcaggagca aggcaaacca ctggaggcgc
     4501 actgggcgca tatggtggtg cacggcagtc tgcatttgtt aggttacgat cacatcgaag
     4561 atgacgaagc agaagaaatg gaagccctcg aaacagagat tatgcttgct ctgggctatg
     4621 aggatccgta cattgccgag aaagaataat ttgccaggca tttgactggc agacgtcccc
     4681 gccgttggtg attaacgacg acgttttgat taactaacat gagaacccat acgacgccat
     4741 gagcgacgac aattcacaca gtagtgacac gataagcaac aagaagggat ttttctccct
     4801 gttactcagc caacttttcc acggtgaacc gaaaaaccgt gacgaactgc tggcgctgat
     4861 ccgtgattcc gggcagaacg accttatcga cgaagatacg cgcgatatgc tcgaaggggt
     4921 gatggacatc gcagaccaac gcgttcgcga catcatgatc ccccgctccc agatgattac
     4981 cctgaaacgc aaccagacgc tggacgaatg ccttgatgtc atcatcgagt ccgcccactc
     5041 acgtttcccg gtgattagcg aagacaaaga tcacattgaa gggattctga tggcgaaaga
     5101 cttgctgccg tttatgcgca gcgatgctga agccttcagc atggacaaag tgttacgtca
     5161 ggcggttgtc gttcctgaaa gtaagcgcgt agaccggatg ctgaaagagt ttcgctctca
     5221 gcgttaccac atggcgatcg tcattgacga attcggtggg gtttccggtc tggtgaccat
     5281 tgaagacatc ctggaactga ttgttggtga gattgaagac gagtatgacg aagaagatga
     5341 tatcgacttc cgtcagctga gtcgtcatac ctggaccgtg cgtgcactgg cttccattga
     5401 agacttcaac gaagcgttcg gcacccactt tagcgatgaa gaggttgaca ctatcggtgg
     5461 tctggtgatg caggcatttg ggcatcttcc ggcgcgtggc gaaactatcg acatcgacgg
     5521 ttaccagttc aaagtggcga tggccgacag tcggcgtatt attcaggttc atgtcaaaat
     5581 cccggatgac tcaccccagc cgaagctgga tgaataaaac cgaaactgga tagataacta
     5641 catggctttt gcctcattaa ttgaacgcca gcgcattcgc ctgctgctgg cgttattatt
     5701 cggtgcctgc ggaacgctgg ccttctctcc ttacgacgtc tggcctgcgg cgattatttc
     5761 gttgatgggg cttcaggcgt tgacctttaa ccgccgtcca ctccagtctg ccgctattgg
     5821 cttttgctgg ggatttggcc tctttggcag cggtattaac tgggtctatg tcagcatcgc
     5881 gacctttggc ggaatgcctg gcccggttaa catcttcctg gtggtgctgc tggcggcgta
     5941 tttgtcgctg tataccggac tgtttgctgg cgtgctgtcg cgtctgtggc cgaaaaccac
     6001 ctggctgcgc gtggcgattg ccgcccctgc tctctggcaa gtgaccgagt ttctgcgcgg
     6061 ttgggtactg acaggcttcc cgtggttaca gttcggctat agccagattg atggcccgtt
     6121 aaaagggctg gcaccgataa tgggcgtgga agccattaac ttcctgctga tgatggttag
     6181 tggcctgctg gcactggcgt tggtcaaacg caactggcgt ccgctggtgg tggccgtcgt
     6241 gctgtttgcc ctacccttcc cgctgcgtta catccaatgg tttaccccgc aaccggagaa
     6301 aaccattcag gtttcgatgg ttcagggcga tattccgcaa tcactgaaat gggatggaga
     6361 tcagcttctt aatacgctga agatttacta caacgcaacg gctccgctga tgggcaaatc
     6421 atcgttgatt atctggccgg agtcggcgat aaccgatctg gaaattaatc agcaaccgtt
     6481 cctcaaagca ctggacggtg agttgcgcga taaaggtagc tcgctggtaa ccgggattgt
     6541 cgacgcgcgt ctcaataagc agaaccgcta cgatacctac aacaccatca tcacactggg
     6601 taaaggtgca ccatacagct acgaatcagc cgatcgctat aacaaaaacc atctagtgcc
     6661 gtttggcgag tttgtcccgc tggagtcgat tctgcgtccg ttagcaccgt tctttgatct
     6721 gccgatgtcg tcgttcagcc gtgggccata tatccagccg ccgctgtcgg taaatggtat
     6781 tgagcttact gcggctattt gctacgagat cattctcggc gagcaagtgc gcgataactt
     6841 ccgcccggat accgactatc tgctgactat ctccaacgat gcgtggtttg gtaagtctat
     6901 tggtccatgg cagcacttcc agatggcgcg aatgcgtgcg ctggagctgg cgcgcccact
     6961 gttgcgcagc accaacaacg gcattacggc ggtgattggc ccgcaggggg aggttcaggc
     7021 gatgatcccg cagttcaccc gcgaggtgtt gaccactaat gtgacaccga ccaccggact
     7081 cacaccgtat gcacgtaccg gcaactggcc actgtgggtg ctgacggcgc tgtttggttt
     7141 tgccgctgtg ttgatgagtc tgcgagcacg taaacgttga tgtgcctgat gtatcctgca
     7201 tgagcaaaac gtactttctc attgagcttt ggggcatggt tatcatgccc ctttttctta
     7261 cggttcaggg atacaagcca cattgccata ttggggatga cgaataattg cctgcggatc
     7321 aagcgagcgc gtcctgtcca tttggatcaa cccgttcttc gccatgacaa atatttttct
     7381 accatccgct gaaaaacgaa ggtgattaac gtagctggca ggataaacgc caataatctg
     7441 cccggcatta atattgagca ggatgacata accttgcagg ccatatgaca ccaacgctaa
     7501 atattgaccg tccgggctaa taccctcaac ggaataaacg ctcctgctac accattgcgt
     7561 cataatgctg gcttgttgat cccctacgct tatacgtgaa atcccctgac tatcggcaaa
     7621 aataacctcg ccgttttcag gtcgaaaacg catcgccgat atcgttccac gcgcatcacc
     7681 tgcgggaaag tcttctttct gatgcgtgat ggtattgatc cgcacgatgt tacctctgcc
     7741 tgctcccgga accagtaatg ttctttcatc cggggaaagt gccataaccc aggcgcctgg
     7801 tttaccgctc gtccccgtcc cgtcgtattt ctccagcggg atatccgcca catgctgata
     7861 agtcaccaga tcaatcgctg gcacactatt actggtgcga ttcatcaccc aggcttttgt
     7921 gccatcacgc gagaggataa ggctgtcagg atgtgcaccg gtggtaatcg actgaataat
     7981 ctcccctgtc gccacgttaa tgaccgataa cgcattcgct tcaaatgaag tcgcataaat
     8041 caacttttca tccgggctta atgccagcga tctgacgccc tgatctttac agccaccaca
     8101 atgtttgact gacttttctt tcgctatggg cgctgtaaag gtatgcagga ttttcttttg
     8161 tccgaggtcg aaaacaacaa tgctgttcgt gttttcaatt gcggcataca acatattacc
     8221 atcccgactg atcgccatat catggatatc ggcaatctca gcgagcaaat caccactggc
     8281 ctgttcacga cttaccggag cggataatgt ttccctgagg gcgttgcgtt cctccgctaa
     8341 ctgctgcttt ttttgccgtc gctcttgttc aatctcctgt ggataaaacg cttcacgcag
     8401 gctttgcctg acctgtaaaa ctgactcatc catctgctgc tgcgcaagcc agatagcccc
     8461 ggtcagaaga agactgcccg caacaataat cccgccttcc gtaacgagtc ggttttgccc
     8521 agataaacgc gcgctcagcc aacccaacgc gccacccgca atcaggccgc caatgtggca
     8581 agcgttatct attcctgttt gccgggcacc gtacaacagt gtcagcgcca ccatacccaa
     8641 cagaggaaat actcgccgct ggtttttatg gtatgtgccc gtacctgaag caaactgtgt
     8701 tgctatcgct gcaccagcta ttcccagaat cgccccggat gccccaacgc tgatgactat
     8761 ctgctcactt tcgcgtaacg cataacaggc gctgatgagc ccaccaaaga cgccggagaa
     8821 taaccaggta atcaggagtc gccatttccc cagaatctgt tccgccacgc ctcccacgac
     8881 caggaatgca aacatattca tcagcagatg agaaacacta ctgtgcagaa agatactgct
     8941 caataagcgc cagggttcac ccgacaaagt aagaggcgca acgtttgcgc cgagatggat
     9001 aagtgcggat tgatcgacct cttcataact catccattgt gcgagataac aaaatatccc
     9061 aatacagagt gcaaaaagga taagggttat tgcgggtaat tgaacattta acggcttaac
     9121 cgaagatgca gacataaggt gtttcttcct tgaaaagaca ggtaaaaagc atttttcccg
     9181 tgtgcagtta tcatcccggc aaacgtccca tgccgaaaag ataattctgt ccgaaatatg
     9241 actttttcag cctaaaatta caatcgtaat taattcgccc ctaagtattt tttggcatgt
     9301 aagttgcttt atttactgtg taagcgatga aatcagccgc gacgcaacca ggttgcacca
     9361 ccacaaatca gcggtagcac caaaaaggtg cggtttaaga attttgcttc ggaatggtgc
     9421 ggcgcatctc gcaaaaacaa acaataaaac agcaatatct ttacatttag ccaaactaaa
     9481 tgttacctaa caggcacaac actgcacaat aaagttgcag acgataacaa cacaaacact
     9541 cacaacgggt atccatgcgt tcttaacgca gaagataaag gagttggata tgcaattacg
     9601 taaacctgcc acagcaatcc tcgccctggc gctttccgca ggactggcac aggcagatga
     9661 cgccgccccg gcagcaggca gtacgctgga caaaatcgcc aaaaacggtg tgattgtcgt
     9721 cggtcaccgt gaatcttcag tacctttctc ttattacgac aatcagcaaa aagtggtggg
     9781 ttactcgcag gattactcca acgccattgt tgaagcagtg aaaaagaaac tcaacaaacc
     9841 ggacttgcag gtaaaactga ttccgattac ctcacaaaac cgtattccac tgctgcaaaa
     9901 cggcactttc gattttgaat gtggttctac caccaacaac gtcgaacgcc aaaaacaggc
     9961 ggctttctct gatactattt tcgtggtcgg tacgcgcctg ctgaccaaaa agggcggcga
    10021 tatcaaagat tttgccgacc tgaaagacaa agccgtagtc gtcacttccg gcactacctc
    10081 tgaagttttg ctcaacaaac tgaatgaaga gcaaaaaatg aatatgcgca tcatcagcgc
    10141 caaagatcac agtgactctt tccgcaccct ggaaagcggt cgtgccgttg cctttatgat
    10201 ggatgatgcc ctgctggccg gtgaacgtgc gaaagcgaag aaaccagaca actgggaaat
    10261 cgtcggcaag ccgcagtctc aggaagccta cggttgcatg ttgcgtaaag acgatccgca
    10321 gttcaaaaag ctgatggatg acaccaccgc gcaggtgcag acctccggtg aagcggaaaa
    10381 atggtttgat aaatggttca aaaatcccat tccgccgaaa aacctgaaca tgaatttcga
    10441 actgtcagac gaaatgaaag cactgttcaa agaaccgaat gacaaggcac tgaactaatt
    10501 acaagaacca ggggcggaaa ttccagccct ctcgattgtt acgtagcacg gacagactat
    10561 acgcctgatg gtcgttcccc atcgggcctg aaaaccgcaa tacgctgggt aacaatcttc
    10621 gagggtagca gttaacgctg ctaccctctt ttttctggag tagatttatg tctatagact
    10681 ggaactgggg tattttttta caacaagccc cgttcggcaa caccacctat cttggttgga
    10741 tctggagtgg ttttcaggtg acgatcgctt tgtcgatctg cgcctggatt atcgctttcc
    10801 tcgtcggttc atttttcggc attttacgta ccgttccaaa ccgttttctc tccggtctgg
    10861 gtacgttgta tgtcgaactg ttccgcaacg tgccgttgat tgtgcaattc tttacctggt
    10921 atctggtgat cccggagctg ttgccggaga aaatcggcat gtggtttaag gccgagctgg
    10981 atcccaatat tcagtttttc ctttcatcca tgctctgcct ggggctgttt accgccgccc
    11041 gtgtttgcga acaggtacgc gccgccattc agtcgttgcc gcgtgggcaa aaaaatgccg
    11101 cgctggcgat gggcctgacg ctaccgcagg cttatcgata cgtactgctg cctaatgctt
    11161 atcgcgttat cgtcccgccg atgacttccg agatgatgaa cctggtgaaa aactccgcca
    11221 tcgcctcgac tattggtctg gtggatatgg cggcgcaggc gggtaaattg cttgattact
    11281 cagcccacgc ctgggagtcg tttaccgcta ttacgctggc gtatgtgttg attaacgcgg
    11341 ttatcatgct ggtaatgacg ctggttgaac gtaaagttcg cctgcctggc aacatggggg
    11401 gcaaataatg tacgagtttg actggagttc cattgtccct tccctgccat atctgctcga
    11461 cgggctggtg atcacgctga aaatcaccgt cacggcagtc gtgattggta ttttgtgggg
    11521 caccatgctg gcggtgatgc gcttgtccag ctttgcgccc gtagcgtggt ttgccaaagc
    11581 ctacgttaac gtattccgct cgattccttt agttatggtt ttgctgtggt tttacctgat
    11641 cgtgccaggt tttctgcaaa acgtgctggg attatcacca aaaaatgata tccgtttaat
    11701 ttcggcgatg gtggcgtttt cgatgtttga agcggcctac tattcagaga ttatccgtgc
    11761 tggtattcaa agtatttctc gtggtcagtc gagcgccgcg ctggcgttgg gaatgactca
    11821 ctggcagtcg atgaaactga ttattctgcc gcaggcgttc cgcgcgatgg tgccgcttct
    11881 gctcactcag ggcatcgtac tgttccagga tacctcactg gtgtatgtgt tatctctggc
    11941 cgatttcttc cgtaccgcct caaccattgg tgagcgtgat ggtacgcagg ttgagatgat
    12001 cctgtttgcc ggatttgttt atttcgttat tagtcttagc gcgtcgttgt tggtcagcta
    12061 cttgaaaaga aggacagcat aatgattacc ctgaaaaatg tttcaaaatg gtatggtcac
    12121 tttcaggtgc tgaccgactg ctcaaccgaa gtgaaaaaag gcgaagtggt ggtggtttgc
    12181 ggcccgtctg gttccggcaa atcaacgctg attaaaaccg tcaacggcct cgaaccagtg
    12241 cagcaaggtg aaatcaccgt cgatggtatc gtggttaatg acaagaaaac cgatctggca
    12301 aagctgcgtt cccgcgtcgg gatggtattc cagcatttcg agctgttccc tcatctgtcg
    12361 attatcgaaa acctgaccct ggcgcaggtg aaagtgctta aacgcgataa agcgcccgct
    12421 cgtgaaaaag cattgaaatt gctggagcgc gtgggacttt ccgcacatgc caataaattt
    12481 ccggcgcagc tttccggcgg tcagcagcag cgtgtggcaa tcgctcgcgc attgtgtatg
    12541 gatcctattg cgatgctgtt cgacgaaccg acatcggcgc tggatccgga gatgatcaac
    12601 gaagtgctgg acgtgatggt ggaactggcg aacgaaggaa tgaccatgat ggtggtgacc
    12661 cacgaaatgg gctttgcccg caaagtggcg aatcgggtga tctttatgga cgagggtaaa
    12721 attgtcgaag actcgccgaa agacgctttc ttcgatgatc cgaaatcgga ccgcgcgaaa
    12781 gacttcctcg cgaaaatcct gcattaatca ctctggcgcg catacccaag tgcgcgccat
    12841 ctcacgcttt atcgtttcgc ccctcgtatc tgttacatcc tggcgttacc cttgtcgcaa
    12901 agaagcacac aacaaggagc aacaatggca ctgccaattc tgttagattg cgacccaggt
    12961 catgacgacg ctatcgcaat agttctcgcc ctcgcctcac cagagcttga tgtcaaagca
    13021 attacgtctt ccgccggaaa ccagacacca gaaaaaacct tacgcaatgt tctgcgtatg
    13081 ctgaccttgc ttaatcgcac cgatattccg gtagcaggcg gcgcggtaaa accgttaatg
    13141 cgtgagttga ttatcgcgga caatgtgcac ggcgaaagcg gtctggatgg cccggcatta
    13201 ccggaaccga cgttcgcacc gcaaaactgt acggcggtag agctgatggc gaaaacgctg
    13261 cgcgaaagtg cggaacctgt cacgattgtg tctaccggac cgcaaactaa cgttgccttg
    13321 ctgctcaata gccatccgga actgcatagc aaaattgccc gtatcgtgat tatgggcggc
    13381 gcaatggggc ttggtaactg gacgcctgcg gctgaattta atatttacgt tgacccggaa
    13441 gcggcagaaa ttgtcttcca gtcagggatc ccagtggtga tggccggtct ggatgttact
    13501 cacaaagcac aaatccacgt tgaagacacc gagcgtttcc gcgcgattgg caaccctgtt
    13561 tcaaccattg ttgccgaact gctggatttc ttcctcgaat atcataaaga cgaaaaatgg
    13621 ggctttgtcg gtgcaccgct gcatgaccca tgcaccatcg cctggctgtt gaaacccgag
    13681 ttgtttacca ctgttgagcg ctgggttggc gtggaaacac aggggaaata tagccagggt
    13741 atgacggttg ttgattatta ttatctgact ggcaataaac cgaatgccac tgtaatggtc
    13801 gatgttgatc gtcagggctt tgttgattta ctggctgatc gtctgaaatt ttacgcttaa
    13861 cacacgtcat tgattaagat caccacgcgt tacctgggtg gtcttaatct gtttatctgt
    13921 ttatctcttt ttctttcaag atataaatgc aatggataat gcagaactcg ccattggtat
    13981 cgatctcggc actaccaata gtttaattgc cgtctggaaa gacggtgccg cgcaattaat
    14041 tccaaataag ttcggtgaat atttaacacc atccataatt agcatggatg aaaataatca
    14101 tattttagtc ggaaaaccgg ctgtatcacg gcgtacttcg catccggata aaacggcagc
    14161 gttatttaaa cgtgcaatgg gcagtaatac caactggcgg ttaggcagcg acacatttaa
    14221 cgcgccagaa ctgtcctctt tggtattacg ctcattaaaa gaagatgccg aagaatttct
    14281 gcaacgtccg attaaagatg tggtgatctc cgttccggct tatttcagcg atgaacaacg
    14341 caagcatacc cgtttagcag cggagttagc cgggttaaat gcggtacgct taattaatga
    14401 acccacagca gctgcgatgg catatggcct gcatacgcaa caaaataccc gctcgctggt
    14461 ttttgatctc ggtggcggca cgtttgacgt cacggtgctc gaatacgcca cgccggtgat
    14521 tgaagttcac gcctccgctg gcgataactt cctcggtggc gaagatttta cccatatgtt
    14581 ggtcgatgac gttttaaagc gcgcggatgt cgccaggacc acgcttaacg agagtgaact
    14641 ggcagccttg tacgcctgtg tggaagcggc aaaatgtagc aatcaatcgc cattgcacat
    14701 tcgctggcag taccaggatg aaacgcggga gtgcgaattt tacgagaacg aactggaaga
    14761 tttgtggttg ccgctgctca atcgcttgcg agtgccgatt gaacaggcgt tgcgcgatgc
    14821 acgtctgaag ccgagtcaaa tcgacagtct ggtgctggtt ggcggcgcgt cacaaatgcc
    14881 gctggtgcag cgaatcgccg tgcgtctgtt tggcaaatta ccgtatcaaa gttacgaccc
    14941 gagcaccatt gtcgcgctgg gcgcagcaat ccaggccgcc tgccgcttac gcagtgaaga
    15001 tattgaagag gtgatcctca ctgatatttg cccttactcg ttgggcgttg aagttaaccg
    15061 ccagggcgtt tccggcattt tctcgccaat tattgaacga aacaccacag tacccgtgtc
    15121 gcgtgtagaa acttactcga ccatgcaccc ggaacaggat tctattacgg ttaacgtcta
    15181 tcagggagaa aaccacaaag ttaaaaataa cattctggtt gaatccttcg atgtgccgtt
    15241 gaagaaaacc ggggcttatc agtcgattga tattcgcttt agttatgata tcaacggctt
    15301 gcttgaagtt gacgtgcttc tggaagatgg cagcgttaag tccagagtca ttaatcatag
    15361 cccggtaaca ttgagcgcgc agcagattga agagagtcgg acgcggttat ccgcattgaa
    15421 aatttatccg cgcgatatgc taatcaaccg cacctttaag gccaaactgg aagagttgtg
    15481 ggcgcgggcg ctgggtgacg agcgagaaga gattggccgg gtgatcaccg attttgatgc
    15541 ggcgttgcag tcaaacgata tggcccgcgt cgatgaagtt cgtcgacggg cgagcgatta
    15601 tttggctatt gagatccctt aaacacggct gccggatgcg gcataaacgc tgcatccggc
    15661 atcggcgcaa aaaacacatc ctcagctaaa caacatcgcg atttttccga gcgcataacc
    15721 tatcatcagc accgttaagc tgattttgat aatactggta atgctctgtg gtttttcatc
    15781 ctcatcattt ttttcttcat ggttttcagg tgttggctca tcgacatctt catcctccgg
    15841 ctcgagtttc actttcgtca acagcatctg ggtgataatg gcctctaatg gatgagcatc
    15901 tccaccttcc agccttgtat taagacgggc cagcatctct tgtttcggga acgcttccag
    15961 cacaacctgt tccgctaccc gatacaaatc cagtaacggt gaatcttgct ctgtctcgct
    16021 accgactctc gatttccacc acgcccatgc tttgcgcttg aagaataacg actcaataaa
    16081 cgattgacct aacggcgata attttccgct atgtaaaatc tccgcccata acagccgcgt
    16141 ccgggcgctc tcaccaggaa catatttcaa cggtttacct tgcgcatcta ccatgcttcg
    16201 cgcttcgata accatcacta ataacgcgaa ataatccggg caatgacgct tgctccactg
    16261 caacaacaaa ttatcagctt gtgtagatgg gtaactttcc cagtacgcgc agagatcggc
    16321 aagcaggctt tccccctcgc cgcagtacaa acgttgtgca cactggtaat accgggcatc
    16381 ttcgctttct ggttcttccg cttgccactg tttggctaca ggaatgagtt ccgccatacc
    16441 tgtttgcacc gagctgtacc agcgcaacag tttgcgatgt aactttgcat catcaggaat
    16501 gaaccacggt ccttccatcg ccaaaaacgc agtgacatag tcaggatggt aacggcagca
    16561 acgttccagc gcgaagtaat attcaacagt ctggttctgt aacgcgacgg gatattccag
    16621 aaggctaagg aaatcgaaac aatcaccagc ctgaatggcc tcaagaaaag cctccctttc
    16681 ttcctcatcc attccttcgc tgtcattaaa cgaatgccag ttcaaacgct ctgccaacaa
    16741 agaagcacaa tttaatgaaa gatatcgcgc ttctatagcg attgcacaca gcggccagcg
    16801 taattgatcg acatcctcca tgttccaggt atttaattgc tgaataaatt tctgccaggc
    16861 ggagggctga aaacgatcac tttctgaatc cagtaatgtc ctgaatgcac gtagtatttc
    16921 atgttcagcg gtggcatctt tttcttcatc atcggcttcc tcgacaggat ttaccgccag
    16981 tcgcagcgcc tcttcatagg cctggcgtaa tgctttaaac ccctgcggat cggtttcggg
    17041 atgacacaac ggtaagcgag caagataagc ctggcggata atgtctattt gcgtcgtgct
    17101 ttcaatttcg aggatttgcc aacatgtttt cataggtcgc tcacatcctt aagcgttacc
    17161 gggcattcag gctggacagt taatggttgc cagggaagcg tggcatagaa acggtcgctt
    17221 cccgctgcca gctcttcgaa catccgactc ccggcgctat cgtatttgta cagggttagc
    17281 gcctcgcgta gcgaagcgtt gtcttcgcac tgagaagatt gccagtagag acgccctagt
    17341 gaataggcag aaaaatagtg catccagctg tgatagtaat ggtgagctcg tgcatagacg
    17401 cgcgattgca gccataaact ctcttcttcc gtcagccagt tgttgagcac accgacccgt
    17461 gacagaaacc ccattcgaac ataatcccag gctttgattc ccccttctcc acagcacata
    17521 gcggtttggg caacgaattg ggtataaatc aaccctctct ccgagccacc agcaattaat
    17581 gctttcctct cttcagggga agagcgttgc catgtcagat ataagccagc taaatcattg
    17641 gcatgaccat catcagtcat ctgataaata gtctgaatta aatcatcacg attatttatt
    17701 ccccaggatg atgagagatc gaattctttt atcccagtat acatttttgg cgagtcatgc
    17761 gcgggtttgc aactctgatt aagtatttcc atgggcgcag aaagcgcaaa aagaagatat
    17821 tgttcttcct tattcatttt ttaataatcc cttaagtgca ttaactgact acttttttat
    17881 tattgatgag tataaacttg acgctttcaa aataaaaaga aaatcgaagc attcacacat
    17941 gaataaaaaa tcaatgtata tattcgcaat ttttatagtt gcagcaatta cctgtattag
    18001 ccaacccaag aaaacgacgt tgcgtgataa agccatggtg aattatgcct ttgattattt
    18061 aagatcacca ggcagtctac cgttaaccac ggcagccacg gagctttccg cgattcatgg
    18121 tcactcaaca tcgcaatatc gccttggcga attttatctt catggtagcg acggtaaacc
    18181 actggattat acacaggcga gatactggta tgagcaatca gcagaacagg aaaatccgcg
    18241 cgcacaaagc aaactggggt ggatctacct gaaaggcctg ggagtcaaac ccgatacccg
    18301 taaagcaatt ctctggtata aggaagcagc tgaacaaggg tatgctcatg ctcaatatac
    18361 tttgggtttg atctacagaa atggcactgg tattaatgtt aaccattatg aatctcaaaa
    18421 atggttaaaa ctggccgcca aacaacatta caaaaatgcg gaaagattac ttgccggact
    18481 tcccgcacat taacgtcgac tgtcacccga agagatggag tatttttcct atcaaaccgg
    18541 caaagataaa aatgtagaaa ataattttga tgacgcccag acagcccagc ccgccatcgt
    18601 caacgggcat tggctgcggt tgcggctccg gctcgacggt ttcctgaccg gtaaaattcg
    18661 ccgcctgaat taataatcga cgaataatta atgcttccag tggacaacga tcttctgcat
    18721 caaggcggct cacaatcggc attaatgatt cttctgtcgg ttggctggcc tgcactaatt
    18781 gtttggcaac taaatataat ggccataagg gatgttctgt atcacgcgtg agtgagatcc
    18841 gcggcttacg tcctttgtgc aaaaccatct cgaccagtga acgccccagc gcagagagtt
    18901 tttcatcgct gagtatttcg tataaatgaa aacgcgtacg ggcgctgtcg ccggggacat
    18961 aaagtagctg tttaccctga tcgttaacca gttcacgagc ttcaatcatc atcaccacta
    19021 acgggtaata atcgacccga tgctgacggc accattgcaa tattaaagca tccgcctggg
    19081 tggagggata ctcgcgccag tagtcacaaa gttctgccag caagctttcg ccttctccac
    19141 aatagacgcg ttgcgcgtat tcataataac gcgcatcttc attatcaggt tcagcttccc
    19201 gccacgaccg ggcgtaatcc agaagttcgg ggatatccag gcgacctgcg ctataccagc
    19261 ggagtaaatt tctgtgcagt tttgcatcat cggggatgat cactgcgcga tgttgcgcta
    19321 aatacaatgt cagccattct ggatgcgatg accaaatccg agcgaacatt tggtaaaaat
    19381 cgatggtgtc attttgcacg gcaacgggca gatgcagaat ggtctggaag ttaaaaacat
    19441 tccccttcgc aatggcatat aaaaaggatt ccagttcttc ttcgtctatt tccccgacgt
    19501 tttcctcctg caaccaccgc aatctttctg ctaataacac cacgcactcg aaggataaat
    19561 gggcagtgtt catggcgatt gtgcacagcg accagcgtaa ttcatcaatc tcttccatcg
    19621 agcaataatt taattgctga atgaatcgct gccaggcgga gggcagaaaa cgttcgctat
    19681 cagaggcaag taacgcgcga aaggcgagca gaatttcatg ttctgctacc tcatattctt
    19741 ctggttgcca aacagattta gcaggcgact gcgcaatccg gagcgcctcc tcatacgcct
    19801 gacgaagttg tttaaaaccc tgcggatcgg tttctggatg aaaggacagt aacagcgcca
    19861 gataagcgcg gcggataata tcgacgtcag tcgtttcctc tatttcgagg atcttccagc
    19921 aatttttcat aggtcgctca tatccttaag cgtttccggg catgtggggt agtcagccag
    19981 tggttgccag gacaaggcag ggtaatacgc atcatcactg gcaaataatt gataaaacat
    20041 atcattgccg gaatcgtcgt attctttacg agctaagaat tcgcgtaata aaggcagatg
    20101 atcatcttcg ggagactgcc aatattgccg accaaaggta taaccggcaa agtattgccg
    20161 ccagttacta taataacgta gagcccgtag atgaatacgc gactggatcc atagactttc
    20221 ttcttcactc aaccagttat taagtacgcc cattcgactt aaaaaaccca tccggacata
    20281 atcccatgct ttaattcctc cctcaccaca gcattcggct gtgctggcga caaattgcgc
    20341 ataagcctga ccttgctcat ttagttcagc aagatagtca cgccattcac atggcgaata
    20401 acgaaaccag cggtgataaa accctgccaa ccgagcggca tgaccgttat cgatcattcg
    20461 gtgaattgtg cgcagcaggt catcacggtc atcaattccc catgaggaag aaaggtcaaa
    20521 aaagcatgta cctaaataca ttttggggga tgaatgcgaa ggtaaacagc attcattgcg
    20581 tatttccatc ggagtggaaa gtgcaaacaa tattttttgc gaccccatgt ccatataatt
    20641 tatccttaaa cattcattta atgaatgtaa actacatttc gagactcagt tatttatact
    20701 tttctgacgc aacataatac gcataaaata aggcttacgc aaaaatggaa cacataaaaa
    20761 tataatatgt tttatctaca accataagga aatgataatg gtttttacgt caagttgctg
    20821 cgataattta tcaatagatg agattatcga acgtgctgaa aaaggcgatt gcgaggctca
    20881 atatattgtt gggttttatt ataatcgcga tagcgcaatt gactctccgg acgacgaaaa
    20941 ggccttttac tggctgaagc tggccgctga gcaaggtcat tgtgaagcac agtattcctt
    21001 agggcaaaag tataccgagg ataaaagccg tcataaagat aatgagcaag ccatcttctg
    21061 gctgaaaaaa gctgccctac aaggccatac tttcgcttcc aacgcccttg gctggacact
    21121 ggatcgtgga gaagccccca attataaaga agcggttgtc tggtatcaga tagccgcgga
    21181 gagcggaatg tcttatgcgc aaaataatct tgggtggatg tacagaaatg gcaacggagt
    21241 cgcaaaagac tatgcgctgg catttttctg gtacaaacaa gctgcattac aaggccatag
    21301 tgacgcgcaa aacaatctgg ccgatcttta tgaagacgga aaaggcgttg ctcaaaacaa
    21361 gacactcgcc gcattctggt atttgaaaag cgcacagcag ggtaatcggc acgcccagtt
    21421 tcaaattgca tgggattata acgctggcga aggggtggac caggactata agcaagcgat
    21481 gtactggtat ctgaaggctg ccgctcagga aagcgtcggc gcttacgtca acatcggtta
    21541 tatgtataaa cacggacaag gcgttgagaa agattatcag gctgcctttg aatggtttac
    21601 gaaagccgct gaatgcaatg acgccactgc ctggtataac ctggccatta tgtatcacta
    21661 cggagaagga agacctgtcg atctccgaca ggctctcgac ctgtatcgta aagttcagtc
    21721 atccggaacc agagatgtca gtcaggaaat tcgtgagact gaagatttac tgtagagata
    21781 aaaacaaggg ccggggaatt tcattcggcc catattctct ttattgctgt gcgaaatcag
    21841 actattacgg ctcaaacggg cgtctctgga actggtcatg accacatttc gggcatagcg
    21901 tcagcacttc cggtgtgtag atcgggagat ggaagtgaca tttctcgcag accagatttc
    21961 ccagcccgac cacttctccg ctgtgataaa ccccatgatg attgaggtcc tggaaaactt
    22021 cgcgccattc aagctgcgtt ttatcggtga tatctgccag ctcctgccat aagctttctt
    22081 taatcacccg cataaagacg ctgtcagatt cttctttcag gctctcttca tagctcatgg
    22141 cgaactcttc caggtcacgt cttacagctc gcgtcagctc atcgacctcg gttcgcgtta
    22201 actcccctgt ttttattacg cgctcgcgcg cctgttccac cagtgcgtcg atatcacgtt
    22261 cgccattgcg caggcgttcg ctcagtgacg cgaccagttc acggtaatat tgagcaacct
    22321 tgttcatcgt ttcatctccc gggtcgttaa ctgactataa taatagacgt tctttgttcc
    22381 ttgctttgct aatacgccca caaactctgt aaattaatgt atgaataatc cgggcatcat
    22441 ttcaacgtcg agtgcgcgaa aggctgtttt gacgcggacg tttgggctat gctatgcgga
    22501 tctgaaaaac cacctcaacg ctacatttgt agccgtattg aaaacaggac cactggctgc
    22561 catgcaagag caataccgcc cggaagagat agaatccaaa gtacagcttc attgggatga
    22621 gaagcgcaca tttgaagtaa ccgaagacga gagcaaagag aagtattact gcctgtctat
    22681 gcttccctat ccttctggtc gactacacat gggccacgta cgtaactaca ccatcggtga
    22741 cgtgatcgcc cgctaccagc gtatgctggg caaaaacgtc ctgcagccga tcggctggga
    22801 cgcgtttggt ctgcctgcgg aaggcgcggc ggtgaaaaac aacaccgctc cggcaccgtg
    22861 gacgtacgac aacatcgcgt atatgaaaaa ccagctcaaa atgctgggct ttggttatga
    22921 ctggagccgc gagctggcaa cctgtacgcc ggaatactac cgttgggaac agaaattctt
    22981 caccgagctg tataaaaaag gcctggtata taagaagact tctgcggtca actggtgccc
    23041 gaacgaccag accgtactgg cgaacgaaca agttatcgac ggctgctgct ggcgctgcga
    23101 taccaaagtt gaacgtaaag agatcccgca gtggtttatc aaaatcactg cttacgctga
    23161 cgagctgctc aacgatctgg ataaactgga tcactggcca gacaccgtta aaaccatgca
    23221 gcgtaactgg atcggtcgtt ccgaaggcgt ggagatcacc ttcaacgtta acgactatga
    23281 caacatgctg accgtttaca ctacccgccc ggacaccttt atgggttgta cctacctggc
    23341 ggtagctgcg ggtcatccgc tggcgcagaa agcggcggaa aataatcctg aactggcggc
    23401 ctttattgac gaatgccgta acaccaaagt tgccgaagct gaaatggcga cgatggagaa
    23461 aaaaggcgtc gatactggct ttaaagctgt tcacccatta acgggcgaag aaattcccgt
    23521 ttgggcagca aacttcgtat tgatggagta cggcacgggc gcagttatgg cggttccggg
    23581 tcacgaccag cgcgactacg agtttgcctc taaatacggc ctgaacatca aaccagttat
    23641 cctggctgct gacggctctg agccagatct ttctcagcaa gccctgactg aaaaaggcgt
    23701 gctgttcaac tctggcgaat tcaacggtct tgaccatgaa gcggccttca acgccatcgc
    23761 cgataaactg actgcgatgg gcgttggcga gcgtaaagtg aactaccgcc tgcgcgactg
    23821 gggtgtttct cgtcagcgtt actggggcgc gccgattccg atggtgacgc tggaagacgg
    23881 taccgtaatg ccgaccccgg acgaccagct gccggtgatc ctgccggaag atgtggtgat
    23941 ggacggcatt accagcccga ttaaagcaga tccggagtgg gcaaaaacta ccgttaacgg
    24001 tatgccagcg ctgcgtgaaa ccgacacttt cgacaccttt atggagtcct cctggtacta
    24061 tgcgcgctac acttgcccag agtacaaaga aggtatgctg gattccgaag cggctaacta
    24121 ctggctgccg gtggatatct acattggtgg tatcgaacac gccattatgc acctgctcta
    24181 cttccgcttc ttccacaaac tgatgcgtga tgcaggcatg gtgaactctg acgaaccagc
    24241 gaaacagttg ctgtgtcagg gtatggtgct ggcagatgcc ttctactatg ttggcgaaaa
    24301 cggcgaacgt aactgggttt ccccggttga tgctatcgtt gaacgtgacg agaaaggccg
    24361 tatcgtgaaa gcgaaagatg cggcaggcca tgaactggtt tataccggca tgagcaaaat
    24421 gtccaagtcg aagaacaacg gtatcgaccc gcaggtgatg gttgaacgtt acggcgcgga
    24481 caccgttcgt ctgtttatga tgtttgcttc tccggctgat atgactctcg aatggcagga
    24541 atccggtgtg gaaggggcta accgcttcct gaaacgtgtc tggaaactgg tttacgagca
    24601 cacagcaaaa ggtgatgttg cggcactgaa cgttgatgcg ctgactgaag atcagaaagc
    24661 gctgcgtcgc gatgtgcata aaacgatcgc taaagtgacc gatgatatcg gccgtcgtca
    24721 gaccttcaac accgcaattg cggcgattat ggagctgatg aacaaactgg cgaaagcacc
    24781 aaccgatggc gagcaggatc gcgctctgat gcaggaagcg ctgctggccg ttgtccgtat
    24841 gcttaacccg ttcaccccgc acatctgctt cacgctgtgg caggaactga aaggcgaagg
    24901 cgatatcgac aacgcgccgt ggccggttgc tgacgaaaaa gcgatggtgg aagactccac
    24961 gctggtcgtg gtgcaggtta acggtaaagt ccgtgccaaa atcaccgttc tggtggacgc
    25021 aacggaagaa caggttcgcg aacgtgctgg ccaggaacat ctggtagcaa aatatcttga
    25081 tggcgttacc gtgcgtaaag tgatttacgt accaggtaaa ctcctcaatc tggtcgttgg
    25141 ctaagcgcgg gaggaagcgt gcgatatctg gcaacattgt tgttatctct ggcggtgtta
    25201 atcaccgccg ggtgtggctg gcatctgcgt gataccacgc aggttccttc cactatgaag
    25261 gtcatgatcc tggactcagg cgatccgaac gggccattaa gccgtgcagt gcgtaaccag
    25321 ttacgtctga atggtgtcga gttgcttgat aaagaaacca cgcgtaagga cgttccatcc
    25381 ctgcgtttgg gtaaagtgag catcgcgaaa gataccgcat cggtattccg taacggtcaa
    25441 acagcagagt atcagatgat catgacggtg aatgcgaccg tgttgatccc cggccgtgat
    25501 atctacccga ttagcgccaa agtcttccgt tcgttctttg ataacccgca aatggcgtta
    25561 gcgaaagata acgaacaaga catgatcgta aaagagatgt acgaccgtgc tgccgaacag
    25621 ctgattcgta agctgccaag catccgtgct gcggatattc gttccgacga agaacagacg
    25681 tcgaccacaa cggatactcc ggcaacgcct gcacgcgtct ccaccacgct gggtaactga
    25741 tgattcggtt gtacccggaa caactccgcg cgcagctcaa tgaagggctg cgcgcggcgt
    25801 atcttttact tggtaacgat cctctgttat tgcaggaaag ccaggacgct gttcgtcagg
    25861 tagctgcggc acaaggattc gaagaacacc acactttttc cattgatccc aacactgact
    25921 ggaatgcgat cttttcgtta tgccaggcta tgagtctgtt tgccagtcga caaacgctat
    25981 tgctgttgtt accagaaaac ggaccgaatg cggcgatcaa tgaacaactt ctcacactca
    26041 ccggacttct gcatgacgac ctgctgttga tcgtccgcgg taataaatta agcaaagcgc
    26101 aagaaaatgc cgcctggttt actgctcttg cgaatcgcag cgtgcaggtg acctgtcaga
    26161 caccggagca ggctcagctt ccccgctggg ttactgcgcg cgcaaaacag ctcaacttag
    26221 aactggatga cgcggcaaat caggtgctct gctactgtta tgaaggtaac ctgctggcgc
    26281 tggctcaggc actggagcgt ttatcgctgc tctggccaga cggcaaattg acattaccgc
    26341 gcgttgaaca ggcggtgaat gatgccgcgc atttcacccc ttttcattgg gttgatgctt
    26401 tgttgatggg aaaaagtaag cgcgcattgc atattcttca gcaactgcgt ctggaaggca
    26461 gcgaaccggt tattttgttg cgcacattac aacgtgaact gttgttactg gttaacctga
    26521 aacgccagtc tgcccatacg ccactgcgtg cgttgtttga taagcatcgg gtatggcaga
    26581 accgccgggg catgatgggc gaggcgttaa atcgcttaag tcagacgcag ttacgtcagg
    26641 ccgtgcaact cctgacacga acggaactca ccctcaaaca agattacggt cagtcagtgt
    26701 gggcagagct gaaagggtta tctcttctgt tgtgccataa acccctggcg gacgtattta
    26761 tcgacggttg atatgaaatc tttacaggct ctgtttggcg gcacctttga tccggtgcac
    26821 tatggtcatc taaaacccgt ggaaacgctg gcgaatttga ttggtctgac gcgggtcaca
    26881 atcatcccta ataatgttcc tccgcatcgt ccccagccgg aagcgaacag cgtgcagcgt
    26941 aaacacatgc ttgaactggc gattgccgac aagccattat ttactcttga tgaacgcgag
    27001 ctaaagcgca atgccccctc ttacactgcg caaacactga aagagtggcg gcaggaacaa
    27061 ggaccggacg tgccgctggc gtttattatt ggtcaggatt cactgctgac ctttccgacc
    27121 tggtacgaat acgaaacgat actcgacaat gcacatttga tcgtctgtcg gcgtccaggt
    27181 tacccacttg aaatggcgca accgcaatac cagcaatggc tggaagatca tttgacacat
    27241 aacccggaag atcttcacct tcagcctgcc ggtaaaattt atctggctga aacgccgtgg
    27301 tttaacatct cggcgaccat catccgcgaa cgtttgcaaa acggtgaatc gtgtgaggat
    27361 ttattgccgg aaccggtact gacttacatt aaccaacaag gcttgtatcg ctgatacctg
    27421 tcgttacgga gggcatgatg cgactgtggt taattcgtca tggtgaaacg caagcgaata
    27481 tcgatggtct ttacagcggt catgcgccca cccccctgac cgcgcgcggt attgagcaag
    27541 cgcaaaatct gcatacgctg ctacatggtg tttcctttga tctggtttta tgcagtgaac
    27601 tggaacgggc acagcatacc gcacgactgg ttctcagtga ccgccagctc cccgtgcaaa
    27661 tcatccctga actcaacgaa atgttttttg gcgactggga gatgcgacat catcgcgacc
    27721 tcatgcaaga agatgccgaa aactatagcg cgtggtgcaa tgactggcag catgcaatcc
    27781 ccacgaacgg tgaaggattt caggcatttt cgcaacgtgt ggaacgcttt atcgcaaggc
    27841 ttagtgaatt tcagcactat cagaatattt tagtcgtcag ccatcagggt gtactgagtc
    27901 tgttaatcgc ccgtttaatt ggcatgcctg ccgaggctat gtggcatttt cgcgttgact
    27961 aggggtgttg gagcgccatt gatatcaacc aaaaattcgc gacgctacgc gtcctcaata
    28021 gccgtgccat cggggtcgag aatgcatgac ttttctgttt ttttacgggt aagccgcaac
    28081 gaccattgac agacccgggc aggctgatat tctccgcagc cagacttttt tcgccagaca
    28141 cgactttgta gaaattgttt tacaaaaatg gcgatgcaat ctgcggcgcg gggtgggatg
    28201 atagcccact ttcgaaagcc gattcggcga caattgtccc gaaatcgcct ctggttcagg
    28261 tatactgaca gaccattttt atctatttga ttcacccagg gggaaaactt gcagggtaaa
    28321 gcactccagg attttgttat cgacaaaatt gatgacctca aaggtcagga catcatcgcc
    28381 ttagacgttc aaggcaaatc cagcatcacc gactgcatga tcatctgtac gggtacgtcc
    28441 agccgtcatg ttatgtccat tgctgaccac gttgtgcagg agtctcgcgc agcgggcctg
    28501 ttaccgctcg gcgtagaagg tgaaaacagc gccgactgga ttgtcgtgga tttgggcgat
    28561 gtgattgtcc atgtcatgca ggaagagagc cgtcgcctgt atgaactgga aaaactctgg
    28621 agttaatgcg tgaagctgca acttgtcgcc gtgggaacga aaatgccgga ctgggtacaa
    28681 accggtttta ccgagtacct gcgtcgtttt ccgaaagata tgcccttcga gctgattgaa
    28741 attccggccg gaaaacgcgg caagaatgcg gacatcaagc gcatactcga caaagagggt
    28801 gagcagatgt tggcggccgc aggcaaaaac cgcattgtca ccctcgatat tccaggcaag
    28861 ccctgggata cgccgcagtt agccgctgag ctggaacgct ggaagctgga tggtcgcgac
    28921 gtcagtctac tgattggcgg gcctgaaggg ttgtcgcctg cctgtaaagc ggcggcggag
    28981 cagagctggt cgctgtcggc gcttaccctc ccccatccgc tggttcgcgt gctggtcgca
    29041 gagagtctgt accgggcgtg gagcatcacc accaaccatc cttatcaccg tgagtgataa
    29101 gggagctttg agtagaaaac gcagcggatg aaactacaga actcttttcg cgactatacg
    29161 gcagagtccg cgctgtttgt gcgccgggcg ctggtcgcct ttttggggat tttgctgctg
    29221 accggcgtgc ttatcgccaa cctgtataat ctgcaaattg ttcgctttac cgactaccag
    29281 actcgctcta atgaaaaccg cattaagctg gtgcctatcg cgcccagccg cggcattatc
    29341 tacgaccgta atggtatccc tctggccctc aaccgcacta tctaccagat agaaatgatg
    29401 ccagagaaag tcgataacgt gcagcaaacg ctggacgctt tgcgcagcgt ggttgatctg
    29461 accgatgacg atattgctgc attccgaaaa gagcgcgcac gttcacaccg tttcacctct
    29521 attccggtga aaactaacct gaccgaagta caagtagctc gctttgccgt caatcagtac
    29581 cgttttccgg gtgtcgaagt taaaggctat aaacgtcgtt actatcctta cggttcggcg
    29641 ttgacccacg tcatcggcta tgtgtcgaaa atcaacgata aagacgtcga acgcctgaat
    29701 aatgacggca aactggccaa ctatgcggca acgcatgata tcggtaagct gggcattgag
    29761 cgttactatg aagacgtgct gcacggtcag accggttatg aagaggttga agttaacaac
    29821 cgtgggcgtg ttatccgcca gttaaaagaa gtaccaccgc aagccggaca cgatatttac
    29881 ctgacgctgg atctcaaact ccagcaatat attgaaacgc tgctggcggg tagccgcgca
    29941 gctgtggttg tcaccgatcc gcgtacaggt ggggtgctgg cgctggtttc cacgcctagt
    30001 tatgacccaa acctgtttgt tgacggtatc tccagcaaag attattccgc cttgttgaac
    30061 gccccgaata caccgctggt gaaccgcgcc acacaggggg tttatcctcc cgcgtctaca
    30121 gttaaaccct atgtggcggt ttcggcattg agcgccgggg tgatcacgcg caatacgacg
    30181 ctgtttgacc caggctggtg gcaactgcca ggttcggaaa aacgttatcg tgactggaaa
    30241 aaatggggcc acgggcgtct gaatgtcaca agatcgctgg aagaatctgc ggataccttc
    30301 ttctatcagg tggcctacga tatggggatc gatcgcctct ccgaatggat gggtaaattc
    30361 ggttatggtc attacaccgg tatcgacctg gcggaagaac gttccggcaa catgcctacc
    30421 cgcgaatgga aacagaaacg ctttaaaaaa ccgtggtatc agggtgacac cattccggtt
    30481 ggtatcggtc agggttactg gacagcgacc ccaatccaga tgagtaaggc actgatgatc
    30541 ctgattaatg acggtatcgt gaaggttcct catttgctga tgagcaccgc cgaagacggc
    30601 aaacaggtgc catgggtaca gccgcatgaa ccgcccgtcg gcgatattca ttccggttac
    30661 tgggagctgg cgaaagacgg tatgtacggt gttgctaacc gccctaacgg tacggcgcat
    30721 aaatactttg ccagcgcacc gtacaaaatt gcggcgaaat ccggtaccgc tcaggtcttc
    30781 ggtctgaaag cgaacgaaac ctataatgcg cacaaaattg ccgagcgttt acgtgaccac
    30841 aaactgatga ccgcctttgc gccatacaac aatccgcaag tggctgtcgc catgattctg
    30901 gagaacggtg gtgcgggtcc ggcggttggt acactgatgc gccagatcct cgaccacatt
    30961 atgctgggtg ataacaacac cgatctgcct gcggaaaatc cagcggttgc cgcagcggag
    31021 gaccattaat catgacggat aatccgaata aaaaaacatt ctgggataaa gtccatctcg
    31081 atcccacaat gctgctgatc ttactggcat tgctggttta cagcgccctg gttatctgga
    31141 gcgccagcgg tcaggatatt ggcatgatgg agcgtaaaat cggccaaatc gcgatgggtc
    31201 tagtcatcat ggtggtgatg gcgcaaattc ctccacgcgt ttatgaaggc tgggccccct
    31261 atctctatat catctgtatt attttgctgg tggcggtaga tgctttcggt gccatctcta
    31321 aaggtgctca acgctggctg gacctcggta ttgttcgttt tcagccgtcg gaaattgcca
    31381 aaatagccgt accactgatg gttgcgcgct ttatcaaccg cgacgtttgc ccgccatcgt
    31441 tgaagaacac tggcatcgcg ctggtgctga tatttatgcc cacgctgctg gtggctgcac
    31501 agcctgacct gggaacatca atcctcgttg cgctttccgg tctgtttgta ctgttcctct
    31561 ctggccttag ctggcgtctg attggcgtcg cagtagtgct ggtggcggcg ttcattccga
    31621 ttctgtggtt cttcctgatg catgattacc agcgccagcg cgtaatgatg ctcctagacc
    31681 cggaatcaga cccactcggc gcgggctatc acattattca gtctaaaatt gctattggct
    31741 ccggcggatt acgcggcaaa ggctggctgc acggcactca gtcacagctt gaatttctcc
    31801 ccgaacgcca taccgacttt atcttcgcgg tactggcgga agagctggga ttagtgggca
    31861 ttctgattct gctcgctctc tacattctgc tgatcatgcg cgggctgtgg atagccgcca
    31921 gagcgcaaac cacctttggt cgcgtcatgg ctggtggctt aatgctgata ttattcgttt
    31981 atgtcttcgt aaatattggt atggtaagcg gtattctgcc ggttgtaggg gttccgctcc
    32041 cactggtcag ttatggagga tcggcgctaa ttgtgctgat ggctgggttc gggattgtaa
    32101 tgtcaatcca cacccacagg aaaatgttgt cgaaaagcgt gtaagaggtg cgcaatgcgt
    32161 aagcagtggc tcgggatctg catcgcggca ggaatgctcg cggcatgtac aagcgatgat
    32221 ggtcagcaac agacggtaag tgtaccgcag cctgcggtat gtaacggccc catagttgaa
    32281 attagcgggg cggacccgcg tttcgaacca ctgaacgcga cggcaaatca ggattaccag
    32341 cgcgacggta aaagctacaa aatcgtgcag gatccgtctc gatttagcca ggcgggactg
    32401 gcggcaatct atgatgccga acccggcagt aacctgacgg cctctggcga agctttcgat
    32461 ccgacacagc tgacggcggc ccatccaacg cttccgatcc ccagctacgc cagaatcact
    32521 aacctggcta acgggcgaat gatcgtggtg cgcattaatg atcgcggccc ttacggcaac
    32581 gacagcgtta tttcactttc tcgcgcggca gctgaccgtc ttaacacgtc aaacaacacc
    32641 aaagttcgta tcgatccgat tattgtcgcc caggatggtt cgctttctgg tcctggtatg
    32701 gcttgtacca cagtcgccaa acagacttac gccctgccag cacctcccga tttaagcggt
    32761 ggcgcgggaa caagttcagt gtctggcccg cagggtgaca ttcttccggt cagtaattcg
    32821 acgctaaaaa gcgaagatcc gaccggcgcg ccggtaacca gtagcggttt cctcggcgct
    32881 ccaacgacct tagcgcctgg tgtactggaa ggcagcgaac cgacgcctgc gccacagccc
    32941 gttgttacag ctccgtcgac aacgcctgca acctcgcctg caatggtgac accgcaagcc
    33001 gcctcgcaaa gcgccagcgg caactttatg gtgcaagtcg gggccgtaag cgatcaggct
    33061 cgtgcgcaac agtaccaaca gcaactggga cagaagttcg gcgtccccgg tcgcgtaact
    33121 caaaatggcg cggtctggcg gatccagctt ggcccattcg ccagcaaagc cgaagccagt
    33181 accttgcagc aacgtttgca aaccgaagcc caattacagt catttattac taccgcgcag
    33241 tagcgtaaag caggcatctg atgtgtcaat ttctgtaaat gacgttaaca aagtcacgcg
    33301 gaaagtcaga tgcctgccgg tagtgcattt gctatagtag ggcacttttt taattccatc
    33361 acggatgtcg tagttcagac catgaatacc attttttccg ctcgtatcat gaagcgcctg
    33421 gcgctcacca cggctctttg cacagccttt atctctgctg cacatgccga tgacctgaat
    33481 atcaaaacta tgatcccggg tgtaccgcag atcgatgcgg agtcctacat cctgattgac
    33541 tataactccg gcaaagtgct cgccgaacag aacgcagatg tccgccgcga tcctgccagc
    33601 ctgaccaaaa tgatgaccag ttacgttatc ggccaggcaa tgaaagccgg taaatttaaa
    33661 gaaactgatt tagtcactat cggcaacgac gcatgggcca ccggtaaccc ggtgtttaaa
    33721 ggttcttcgc tgatgttcct caaaccgggc atgcaggttc cggtttctca gctgatccgc
    33781 ggtattaacc tgcaatcggg taacgatgct tgtgtcgcca tggccgattt tgccgctggt
    33841 agccaggacg cttttgttgg cttgatgaac agctacgtta acgcactggg cctgaaaaat
    33901 acccacttcc agacggtaca tggtctggat gctgatggtc agtacagctc cgcgcgagat
    33961 atggcgctga tcggccaggc attgatccgt gacgtaccga atgaatactc gatctataaa
    34021 gaaaaagaat ttacgtttaa cggtattcgc cagctgaacc gtaacggcct gttatgggat
    34081 aacagcctga atgtcgacgg catcaaaacc ggacacactg acaaagcagg ttacaacctt
    34141 gttgcttctg cgactgaagg ccagatgcgc ttgatttctg cggtgatggg cggacgtact
    34201 tttaaaggcc gtgaagccga aagtaaaaaa ctgctaacct ggggcttccg tttcttcgaa
    34261 accgttaacc cactgaaagt aggtaaagag ttcgcctctg aaccggtttg gtttggtgat
    34321 tctgatcgcg cttcgttagg ggttgataaa gacgtgtacc tgaccattcc acgtggtcgc
    34381 atgaaagatc tgaaagccag ctatgtgctg aacagcagtg aattgcatgc gccgctgcaa
    34441 aagaatcagg tcgtcggaac tatcaacttc cagcttgatg gcaaaacgat cgagcaacgc
    34501 ccgctggttg tgttgcaaga aatcccggaa ggtaacttct tcggcaaaat cattgattac
    34561 attaaattaa tgttccatca ctggtttggt taaaaattga acacttgaaa gtgtaatttc
    34621 cgtccccata tactaagcat cagtaaaaaa ctcccgcctt ctggcgggag ttgctattta
    34681 attacgttac gccggagctg acatgaaaac caaacttaac gaactgcttg aattccctac
    34741 tccttttact tacaaagtta tggggcaggc gttacctgag ctggttgatc aggtggttga
    34801 agtggtacag cgccatgcgc caggtgacta caccccaacg gtaaaaccaa gcagcaaagg
    34861 caactaccac tcggtatcta tcactatcaa cgccactcat atcgagcagg ttgaaacact
    34921 gtatgaagaa ctaggcaaaa tcgatattgt ccgcatggtt ctgtaactcg cttctccgtt
    34981 acccggcccc tggtcgggta actcccctct cctcgctgtg atatactttt cccccacttt
    35041 tactcattct ccacggagat gcagttttgt atcaggataa aattcttgtc cgccagctcg
    35101 gtcttcagcc ttacgagcca atctcccagg ctatgcatga attcaccgat acccgcgatg
    35161 atagtaccct tgatgaaatc tggctggtcg agcactatcc ggtattcacc caaggtcagg
    35221 caggaaaagc ggagcacatt ttaatgccgg gtgatattcc ggtgatccag agcgatcgcg
    35281 gtgggcaggt gacttatcac gggccggggc aacaggtgat gtatgtgttg cttaacctga
    35341 aacgccgtaa actcggtgtg cgtgaactgg tgaccttgct tgagcaaaca gtggtgaata
    35401 ccctggctga actgggtata gaagcgcatc ctcgggctga cgcgcctggt gtctatgtcg
    35461 gggaaaagaa aatttgctca ctgggtttac gtattcgacg cggttgttca ttccacggtc
    35521 tggcattaaa cgtcaatatg gatctttcac catttttacg tattaatcct tgtgggtatg
    35581 ccggaatgga aatggctaaa atatcacaat ggaaacccga agcgacgact aataatattg
    35641 caccacgttt actggaaaat attttagcgc tactaaacaa tcctgacttc gaatatatta
    35701 ccgcttaatt gcatacctca atggcccaat ttacactggg tcattactca taatacctgc
    35761 taatgcttta catctccttc gcgtttgtat actttcttta tcacttgcag aaatgcggtg
    35821 ctacgccttg ctccgtaact caccccattg attaacctat tgaaatagtt gattatggtg
    35881 agcaaaagcg ttccgttcca attagacata agattattgt aattttttta aatattattt
    35941 ttccatgaat aaaattggag taagtgcgtg gatagtaata atcaaattga accctgttta
    36001 agcagaaagt cgtcagaagg taaaccacaa atatttacaa ccttacgaaa tattgatctt
    36061 aaccttctga ctatttttga agctgtatat gtacataaag ggatcgttaa tgcagcgaaa
    36121 gtgcttaatc tgaccccctc ggcaatcagt cagtctattc agaaactgcg cgttatattc
    36181 cctgacccat tgtttattcg caaaggccag ggtgtcactc ctaccgcatt tgcgatgcat
    36241 ctacatgagt atatcagtca gggccttgag tccattcttg gcgcgctgga tatcgaagga
    36301 agctatgata agcaacgaac gataactatt gcgaccactc cctcggtcgg agccctggtc
    36361 cttcctgtca tctatcgggc gattaaaact cactatccgc agcttttact gcgcaacccg
    36421 cccatcagcg acgcggaaaa ccaactcagt cagtttcaaa ccgatctcat catcgataac
    36481 atgttttgca ccaatcgtac ggtgcaacat catgttctgt tcaccgacaa tatggtgtta
    36541 atttgccgcg agggaaatcc actactctct ttagaagatg acagagagac tatcgacaac
    36601 gctgcgcatg tactcctgtt accggaaggg caaaatttca gcggtctgcg gcagagagtt
    36661 caagagatgt ttccggaccg gcaaatcaat ttcaccagct acaacatttt gacaatcgct
    36721 gcactggttg ccaacagtga catgttagcg attattccca gccgttttta taacctgttt
    36781 agtcgttgct ggccgctgga aaaactgcct tttccgtcct taaatgagga gcaaatagat
    36841 ttctctatcc actacaacaa attcagcctg cgtgatccca tcctgcacgg ggtaatagat
    36901 gttatccgta atgcattttg aaaaagtatt gttacagcca gatcacagcg cagaacgata
    36961 aaatggcaca atccaacaac aattttacat tttcgcgacc gctttggctg ctttcaggtc
    37021 cgtttcaatg atatactgcc agtcgttaat tcaaaaatag ttgataatta caacaatcta
    37081 ttgaattgaa acgctttcct tcgtaattcg caactggaac acgcacgcta tgagtaaacc
    37141 cattgtgatg gaacgcggtg ttaaataccg cgatgccgat aagatggccc ttatcccggt
    37201 taaaaacgtg gcaacagagc gcgaagccct gctgcgcaag ccggaatgga tgaaaatcaa
    37261 gcttccagcg gactctacac gtatccaggg catcaaagcc gcaatgcgca aaaatggcct
    37321 gcattctgtc tgcgaggaag cctcctgccc taacctggcg gaatgcttca accacggcac
    37381 agcaacgttt atgatcctcg gcgctatttg tacccgccgt tgtccgttct gtgatgttgc
    37441 ccacggtcgc ccggtagctc ctgatgccaa tgaaccagtg aaactggcgc agaccattgc
    37501 cgatatggcg ctgcgttatg tggttatcac ctccgttgac cgtgatgacc tgcgcgatgg
    37561 cggtgcccag cactttgcgg attgcattac tgccattcgg gaaaaaagcc cgcaaatcaa
    37621 aattgaaact ctggtgccgg atttccgtgg tcgtatggat cgtgctctgg atattctgac
    37681 tgcaacgcca ccagatgtgt tcaaccataa cctggaaaac gtaccgcgta tttaccgtca
    37741 ggtacggcct ggtgcagatt acaactggtc gctgaagctg ctggaacgct ttaaagaagc
    37801 gcatccggaa atcccgacca agtctggtct gatggtggga ctgggtgaaa ccaatgaaga
    37861 aattattgag gtaatgcgcg acctgcgccg tcatggtgtg acgatgttaa cgctggggca
    37921 atatttgcag ccaagccgcc atcacctgcc ggttcaacgt tacgttagcc cggatgagtt
    37981 cgacgaaatg aaagccgaag cgctggcgat gggctttacc catgctgcat gcggtccgtt
    38041 tgtccgctct tcttaccacg ccgatttgca ggcgaaaggg atggaagtta agtaatcctg
    38101 taataattac ttacatctgt ctgataaaaa acccgctttt tg
//
LOCUS       DAJTZL010000020        37269 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.020, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000020 DAJTZL010000000
VERSION     DAJTZL010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37269
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.020"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..758)
                     /gene="aceF"
                     /locus_tag="OO544_000880"
     CDS             complement(<1..758)
                     /gene="aceF"
                     /locus_tag="OO544_000880"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="E2 component of pyruvate dehydrogenase multienzyme
                     complex; in Escherichia coli AceF contains three
                     N-terminal lipoyl domains; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HCR6766549.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAAPASAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAP"
     gene            complement(773..3436)
                     /gene="aceE"
                     /locus_tag="OO544_000881"
     CDS             complement(773..3436)
                     /gene="aceE"
                     /locus_tag="OO544_000881"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HCR6766550.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYIPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(3597..4361)
                     /gene="pdhR"
                     /locus_tag="OO544_000882"
     CDS             complement(3597..4361)
                     /gene="pdhR"
                     /locus_tag="OO544_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HCR6766551.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            4902..6275
                     /gene="aroP"
                     /locus_tag="OO544_000883"
     CDS             4902..6275
                     /gene="aroP"
                     /locus_tag="OO544_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HCR6766552.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAIVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(6318..7172)
                     /gene="ampE"
                     /locus_tag="OO544_000884"
     CDS             complement(6318..7172)
                     /gene="ampE"
                     /locus_tag="OO544_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HCR6766553.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(7169..7720)
                     /gene="ampD"
                     /locus_tag="OO544_000885"
     CDS             complement(7169..7720)
                     /gene="ampD"
                     /locus_tag="OO544_000885"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HCR6766554.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            7808..8701
                     /gene="nadC"
                     /locus_tag="OO544_000886"
     CDS             7808..8701
                     /gene="nadC"
                     /locus_tag="OO544_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HCR6766555.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVSTRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            8904..9344
                     /gene="ppdD"
                     /locus_tag="OO544_000887"
     CDS             8904..9344
                     /gene="ppdD"
                     /locus_tag="OO544_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HCR6766556.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            9354..10740
                     /gene="gspE"
                     /locus_tag="OO544_000888"
                     /pseudo
     CDS             9354..10740
                     /gene="gspE"
                     /locus_tag="OO544_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
     gene            10730..11932
                     /gene="hofC"
                     /locus_tag="OO544_000889"
                     /pseudo
     CDS             10730..11932
                     /gene="hofC"
                     /locus_tag="OO544_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414648.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
     gene            complement(11967..13010)
                     /gene="guaC"
                     /locus_tag="OO544_000890"
     CDS             complement(11967..13010)
                     /gene="guaC"
                     /locus_tag="OO544_000890"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HCR6766557.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVQEQENRIFNNL"
     gene            complement(13166..13210)
                     /locus_tag="OO544_000891"
     CDS             complement(13166..13210)
                     /locus_tag="OO544_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6766558.1"
                     /translation="MIIKTLKMSARKRN"
     gene            13235..13855
                     /gene="coaE"
                     /locus_tag="OO544_000892"
     CDS             13235..13855
                     /gene="coaE"
                     /locus_tag="OO544_000892"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HCR6766559.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            13855..14598
                     /gene="zapD"
                     /locus_tag="OO544_000893"
     CDS             13855..14598
                     /gene="zapD"
                     /locus_tag="OO544_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HCR6766560.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            14608..14805
                     /gene="yacG"
                     /locus_tag="OO544_000894"
     CDS             14608..14805
                     /gene="yacG"
                     /locus_tag="OO544_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HCR6766561.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(14905..15294)
                     /gene="mutT"
                     /locus_tag="OO544_000895"
     CDS             complement(14905..15294)
                     /gene="mutT"
                     /locus_tag="OO544_000895"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HCR6766562.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGEPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(15354..18059)
                     /gene="secA"
                     /locus_tag="OO544_000896"
     CDS             complement(15354..18059)
                     /gene="secA"
                     /locus_tag="OO544_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HCR6766563.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(18121..18633)
                     /gene="secM"
                     /locus_tag="OO544_000897"
     CDS             complement(18121..18633)
                     /gene="secM"
                     /locus_tag="OO544_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secA regulator SecM"
                     /protein_id="HCR6766564.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(18864..19781)
                     /gene="lpxC"
                     /locus_tag="OO544_000898"
     CDS             complement(18864..19781)
                     /gene="lpxC"
                     /locus_tag="OO544_000898"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HCR6766565.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(19882..21033)
                     /gene="ftsZ"
                     /locus_tag="OO544_000899"
     CDS             complement(19882..21033)
                     /gene="ftsZ"
                     /locus_tag="OO544_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HCR6766566.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(21094..22356)
                     /gene="ftsA"
                     /locus_tag="OO544_000900"
     CDS             complement(21094..22356)
                     /gene="ftsA"
                     /locus_tag="OO544_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HCR6766567.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(22353..23183)
                     /gene="ftsQ"
                     /locus_tag="OO544_000901"
     CDS             complement(22353..23183)
                     /gene="ftsQ"
                     /locus_tag="OO544_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HCR6766568.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(23185..24105)
                     /gene="ddlB"
                     /locus_tag="OO544_000902"
     CDS             complement(23185..24105)
                     /gene="ddlB"
                     /locus_tag="OO544_000902"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCR6766569.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(24098..25573)
                     /gene="murC"
                     /locus_tag="OO544_000903"
     CDS             complement(24098..25573)
                     /gene="murC"
                     /locus_tag="OO544_000903"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HCR6766570.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLIGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(25627..26694)
                     /gene="murG"
                     /locus_tag="OO544_000904"
     CDS             complement(25627..26694)
                     /gene="murG"
                     /locus_tag="OO544_000904"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HCR6766571.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(26691..27935)
                     /gene="ftsW"
                     /locus_tag="OO544_000905"
     CDS             complement(26691..27935)
                     /gene="ftsW"
                     /locus_tag="OO544_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HCR6766572.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(27935..29251)
                     /gene="murD"
                     /locus_tag="OO544_000906"
     CDS             complement(27935..29251)
                     /gene="murD"
                     /locus_tag="OO544_000906"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HCR6766573.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIAQAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDAECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     QVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQLGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(29254..30336)
                     /gene="mraY"
                     /locus_tag="OO544_000907"
     CDS             complement(29254..30336)
                     /gene="mraY"
                     /locus_tag="OO544_000907"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HCR6766574.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(30330..31688)
                     /gene="murF"
                     /locus_tag="OO544_000908"
     CDS             complement(30330..31688)
                     /gene="murF"
                     /locus_tag="OO544_000908"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HCR6766575.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(31685..33178)
                     /gene="murE"
                     /locus_tag="OO544_000909"
     CDS             complement(31685..33178)
                     /gene="murE"
                     /locus_tag="OO544_000909"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HCR6766576.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKAKDEATDGEIREMHGVPVIYLSQLNERLSALAGR
                     FYHEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENT
                     TGSAVDVQHELAGLVEQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHG
                     DMEHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWL
                     KAIDVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKT
                     AARLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDR
                     DKGKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAV
                     TCAVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(33165..34931)
                     /gene="ftsI"
                     /locus_tag="OO544_000910"
     CDS             complement(33165..34931)
                     /gene="ftsI"
                     /locus_tag="OO544_000910"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HCR6766577.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPGSIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(34947..35312)
                     /gene="ftsL"
                     /locus_tag="OO544_000911"
     CDS             complement(34947..35312)
                     /gene="ftsL"
                     /locus_tag="OO544_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HCR6766578.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(35309..36250)
                     /gene="rsmH"
                     /locus_tag="OO544_000912"
     CDS             complement(35309..36250)
                     /gene="rsmH"
                     /locus_tag="OO544_000912"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HCR6766579.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAVATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(36252..36710)
                     /gene="mraZ"
                     /locus_tag="OO544_000913"
     CDS             complement(36252..36710)
                     /gene="mraZ"
                     /locus_tag="OO544_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HCR6766580.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAVGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
BASE COUNT         8778 a        10403 c         9210 g         8878 t
ORIGIN      
        1 ggagccggga cctccataga agctttatcg ccttctacgg tgataagcga ttgttcagcg
       61 gcaactttgt cgcccacttt caccatcact tcggtcactt caacttcgtc accgccgata
      121 tccggaacgt taacttcttt cacgccagcc gctgatgcag gggccgctgc cggagctgct
      181 tcctgtttag ccgccggagc tgccgcgcct gcttcacccg cgacttcgaa gaccataatc
      241 agcgagccgg tagacacttt gtcacccacg ttcactttga tctctttcac ggtgccagca
      301 aacggagccg gaacttccat agaagcttta tcgccttcta cggtgatcag cgactgttca
      361 gcttcaactt tatcgccaac tttcaccagg atttcggtca cttcaacttc gtcgctgccg
      421 atatccggaa cgttaacgtc ttttgccgcc gcagccgcgg gtgctgctgc cggagctgct
      481 tctttcttct cttctgcctg agcaggtgca gcgtctgctg caccgtcggc ggaatcgaaa
      541 atcataatca gtgcgccggt ctgggtttta tcgccaacag agactttgat ctctttaacg
      601 atacccgcct gcggagacgg aacttccata gaggctttgt cgccttctac ggtgatcagc
      661 gactgttcgg cttcaacttt gtcgcccact ttgaccagga tctcggtgat ttcaacttca
      721 tcagccccga tgtccggtac tttgatttcg atagccatta ttcttttacc tcttacgcca
      781 gacgcgggtt aactttatct gcatcgatgt tgaatttggc gattgcgtca gcaaccactt
      841 tcttatcgat ttcgccacgt ttagccagtt cgcccagcgc cgcaaccacg acataagaag
      901 catcaacttc gaagtggtga cgcaggttct cacggctgtc ggaacgaccg aagccatcag
      961 tacccagtac gcggtagtcg tcagccggta cgtaagtacg gacctgctca gcgaacagtt
     1021 tcatatagtc ggtagatgcc actgccggag cgtcgttcat cacctgagcg atatacggaa
     1081 cgcgcggagt ttccagcgga tgaagcatgt tccagcgttc acaatcctga ccatcacgcg
     1141 ccagctcggt gaaggaggtc acgctataaa cgtcagaacc tacgccgtaa tctttcgcca
     1201 ggatctctgc cgcttcacgg acgtgacgca ggatagaacc ggagcccagc agctgaactt
     1261 tacctttgct accttcaatg gtttcgagtt tgtagatacc tttacggata ccttcctcag
     1321 caccttccgg cattgccggc atgtggtagt tttcgttcag cgtagtgatg tagtagtaaa
     1381 cgttctcttg tttttcaccg tacatacgct ccagaccgtc atgcatgatg acagcaactt
     1441 cgtaagcgta agccgggtcg taagagatac agttcgggat agtcagcgac tgaatgtggc
     1501 tgtgaccatc ttcgtgctgc agaccttcac cgttcagggt ggtacgaccg gaagtaccgc
     1561 cgatcaggaa gccacgcgct tgctggtcgc cagccgccca gcacagatcg ccgatacgct
     1621 ggaagccgaa catcgagtaa tagatgtaga acggaatcat cggcagattg ttggtgctgt
     1681 aagaggtcgc cgctgccagc caggaacacc ctgcgcccag ctcgttgatc ccttcctgca
     1741 gaatctgacc tttctcgtct tctttatagt aagcaacctg ctcgcggtcc tgcgggatgt
     1801 actgctgacc gttcgggctg taaataccaa tctgacggaa cagaccttcc ataccgaaag
     1861 tacgcgcttc gtcggcgatg atcggtacca gacgatcttt gatcgacttg ttcttcagca
     1921 ttacgttcag ggcacgaacg aaagcgatag tggtagagat ctctttgctc tgctcttcca
     1981 gcagcgcgcc gaagtcttgc aggctcggca gctcaagctt ctcggtgaag ttcggctgac
     2041 ggcttggcag ataaccgtgc agtttctgac gctgtgcgtg cagataggta tgctcttcag
     2101 aaccttccgg gaaggtgatg tacggcagtt tttcgatatc tgcatcagac accggcacat
     2161 tgaaacggtc gcggatgtga cgcacgccgt ccatgttcat tttcttaacc tggtgcgcga
     2221 tgtttttacc ttcagccgcg tcgcccatgc cgtaaccttt aatggtatga gcaaggatta
     2281 ctgtcgcttt gcctttggtt tcctgcgctt tcttgaatgc agcgtagatt ttcttcggat
     2341 cgtgaccacc acggttcagt gcccagatct gctcgtcagt ccagtctgca accagtgctg
     2401 cggtttcagg atatttaccg aagaagtgtt cacgaacgta cgcaccatct ttcgatttga
     2461 aggtctggta gtcgccgtca acggtttcgt tcatcagctg gatcagttta ccgctggtat
     2521 ctttacgcag cagttcatcc caacggctac cccacatcac tttgataacg ttccagccag
     2581 caccttcgaa gatgccttcc agttcgttga tgatcttgcc gttaccggtg accgggccgt
     2641 caagacgctg caggttacag ttgataacga agaccaggtt atccagtttt tcacgggtag
     2701 cgatggtgat cgcacctttg gattccggtt cgtccatttc accgtcgccg aggaacgcgt
     2761 aaacggtctg tttagaggta tctttcaggc cacggtgttc cagatatttc aggaatttag
     2821 cctggtaaat agcaccaatc ggccccagac ccatagatac ggtcgggaac tgccagaatt
     2881 ccggcatcag tttcgggtgc ggataggaag agaggccatt gccgtgaact tcctgacgga
     2941 agttatccag ctgctcttga gtcagacgac cttccaggaa agcacgagcg tacacgcccg
     3001 gggagatgtg gccctggaag taaaccaggt cgccgccatc ctgctcgttg cgtgcacgga
     3061 agaagtggtt aaagcacaca tcataaatgg ttgcggaaga ctggaaggac gccatatggc
     3121 cgcccagttc gaggtctttt ttcgacgcac gcagcaccgt catgatggcg ttccagcgga
     3181 tagctgaacg aatacggcgt tccagttcca gattacccgg atactccggt tgttcttcaa
     3241 cggggatggt gttgatgtag ttgctgatac ctgtgcctgc ggctacgttt acaccgcctt
     3301 tgcgggcttc agcaagcagt tggtcgatca gatactgagc acgctcaaca ccttcttcac
     3361 ggatgaccga ttcgatcgcc tggagccagt cgcgagtttc gatcggatcc acgtcatttg
     3421 ggaaacgttc tgacatgggt tattccttat ctatctaata acgttgagtt ttctggaacc
     3481 tgtcccattg aactctcgcc ggaaagctca ataagacagg ttctacgttt agttgccgcg
     3541 cttttatata cgcttgattt acaacatctt ctggataatt tttaccagaa aaatcactaa
     3601 ttctttcgtt gctccagacg acgcagagaa cgctcacggc ggctctcttc acgacttctg
     3661 tcgagcaaaa tttcttcgat aaaggccaga tggcgatgcg atgcttcgcg cgcttcttcc
     3721 ggcttaccgg ccataatcgc ttcaaatatg cgggtgcggt gactactcac cagcggcagc
     3781 atctcgcgac gcgaatagag caattcgaag ttctggcgga cattctgggc caacatcggc
     3841 tccatacacc ttagcagatg aagcagaacc acattgtggg ccgcttcggt gacggcaatc
     3901 tgatactgga gtacggcgtt tgattccgcg tccagatcgc cagactgctg cgccagctct
     3961 atggcgtggt ggagttcacg gatgcgttcc ttgtcttcat cggtactacg cagcgcggcg
     4021 taataagcgg cgataccttc cagggcgtgt cgtgtttcga gcaagtcata ctgtgattca
     4081 ggatggtcgg agagcagctc caccagcgga tcgctgaagc tttgccatag gctgctctgg
     4141 acaaaagtgc cgccaccctg gcgacgaagc aacaagccct tcgcttcgag acgttgaatc
     4201 gcctcacgca aggagggacg ggagacgtcg aactgttttg ccagttcgcg ttccggtggg
     4261 agtttttcgc ccgggcggag agtgccttcg agaatcaaaa actccagttg ctgctcaatc
     4321 acatcggaga gttttggttg gcggattttg ctgtaggcca tgagttcctg tcttaagcca
     4381 cttgccgaag tcaattggtc ttaccaattt catgtctgtg acgctaaagt aacaaagtat
     4441 tcaccttatg tccatacagg ttttgattga aatcatgaaa ctgtgcacat tttaacaact
     4501 tgacatatat aacgtttcaa aattgtaact atgcacaaat gtagacttta cgtaggaaag
     4561 ggatttttta acgattatta aactataaaa atccgaattg aaccgattca cttaccaatt
     4621 ttttgatttt taagtcaata aaaacgaatt taaattcatt ctgcatattg agaggggttg
     4681 aggctgagct ttacaaacgg tttcttttta agcaactcat cttcaaccat gcataaagcg
     4741 ggtgcattcg ctgccgcata ccattattct tgatctgacg gaagtctttt tgtaacaatt
     4801 caaacttctt tgatgtaaaa aaattaatac aacaaacgga attgcaaact tacacacgca
     4861 tcactgcgta gatcaaaaaa acaatcaccg cacgaggttt catgatggaa ggtcaacagc
     4921 acggcgagca gctaaagcgc ggccttaaaa accgccatat tcagcttatc gcgctgggtg
     4981 gcgcgatagg gacagggcta ttcctgggta gcgcctccgt aatacagtcc gcagggccag
     5041 ggattatcct gggttacgcc attgctggtt ttatcgcctt tctgatcatg cgtcagctgg
     5101 gtgaaatggt ggtcgaagaa cctgtcgcag gctcctttag ccactttgct tataaatact
     5161 ggggcagttt tgccggtttc gcctctggct ggaactactg ggtactgtac gttttagttg
     5221 ccatggcaga gctgactgcc gtgggtaaat acattcagtt ctggtatccg gaaatcccaa
     5281 cctgggtttc tgccgccgta ttctttgtgg tgattaacgc catcaacctg accaacgtta
     5341 aagtgtttgg cgagatggag ttctggtttg ccattatcaa agttattgcg atagtagcga
     5401 tgatcatctt cggcggctgg ctgctgttca gtggcaacgg cggcccgcag gcgagcgtta
     5461 gcaacctgtg ggatcaaggt ggtttcctgc cgcacggctt caccggactg gtgatgatga
     5521 tggcgattat catgttctcg ttcggtggtc tggaactggt ggggatcacc gcagcagaag
     5581 ctgataaccc ggagcaaagt atcccgaaag cgactaacca ggttatctac cgcatcctga
     5641 ttttctatat tggttcgtta gccgttctgc tctcactgat gccgtggacc cgcgttaccg
     5701 ccgataccag tccgtttgtg ctgatcttcc acgagttagg cgataccttt gtggcgaatg
     5761 cgctgaacat cgtggtactg actgcggcgc tctccgtgta caacagctgc gtatattgca
     5821 acagccgtat gctgtttggt ctggcacaac agggtaatgc gccaaaagcg ctggcgtctg
     5881 tcgataaacg cggcgtaccg gttaacacca ttctggtgtc tgcgctggtt acagcattgt
     5941 gcgtattgat taactatctt gctccggaat ccgcatttgg cctgttaatg gcactggtgg
     6001 tatccgcact ggtgatcaac tgggcgatga tcagtctggc gcatatgaag ttccgtcgcg
     6061 ccaagcagga acaaggcgtg gtaactcgct tccctgctct gctttatccg ctgggtaact
     6121 ggatctgcct gctgtttatg gcggcggtac tggtgattat gctgatgacc ccaggaatgg
     6181 cgatttcggt atacctgatc ccggtatggc tgatcgtgtt aggtatcggc tatctgttta
     6241 aagagaaaac cgccaaagcc gtaaaagcgc attaatctct ctacgccctc acccatgcag
     6301 ggtgagggca ataatcttta caccaacgct ccgtaaatcg tcagtagcgc aatcaccacc
     6361 acgaccacga acgaggtttt cttcgccatt gaaaccgctg ccttcggcgt ctccacctta
     6421 tcgacatgcg gttcacgcgc cagagagaac tgcgccagac gcgttaacac ctgatactgc
     6481 gaagtatgga agtcacccag cgaagcaaac caggccggta acgctttctc cccatgaccg
     6541 atcaaggcat ataccacacc cgcaagacga accggcaccc aatccagtac atgaagcacg
     6601 gcatcaatgc cggactgtaa acgatgatgc ggcgtctggt atcgcgccag ccagtattgc
     6661 catgcacgca aaaacgcata ccccatcagc gtaacgggtc cccaggttcc ccccacaatc
     6721 aaccagaaca gcggtgcaag ataaaaacga aagttaatcc acagcaatgc attttgcagc
     6781 tcacgcaaat actcacgttc gtcgcagcct gccgggacgc cgtgaatcat ggtgagttcg
     6841 ccagccatcg tggcacgggc atggctatca ttacgtgaag cagctgtcag ataagcatga
     6901 taatgaagac gaactttacc tgcgccaata cacagcaaac caatcagcag ccacaccagc
     6961 agcgtgggaa cgttgaacaa tactccctgc aatgcgcgta acagtaaaaa agtcacgccc
     7021 atcgcaataa tggtcatgcc taacgtgcgc ccgagagaaa aatgtttcac ccgccgaaag
     7081 aacgcttcaa gacgatgatc aagctgccag tgctcgccca acttaaacag gcgctcgaaa
     7141 attaacacca gtaaggttgt aaatagcgtc atgttgtctc cttgctgacc agcgcacgaa
     7201 accgtgccca atcaaatgca ggaccgggat cggttttccg atccggcgca atatcacaat
     7261 ggcccgtcat gttattagcg atatcgggat agcgatctat cagtgcccgc gtaaccgccg
     7321 caagctgttg atactgcgca tcggtatacg ccagagtatc ggtgccttca agctcaatcc
     7381 caatagaaaa atcattgcag cgttcacgcc cctgatactg agagactccc gcatgccagg
     7441 cacgtttatc gaaaggaaca tactggacta tttcaccatc acggcgaatc aaacagtgag
     7501 cggagacgcg caaatgggcg atctcagcaa agaaaggatg tgcctgcgga tcaatagttc
     7561 cagtgaataa tgcgtcgatc cacggaccgc caaactcgcc tggcggcagg ctaatattgt
     7621 gcaccaccag cagggtgggt gtttcgtcat ccgggcggca atcgtaatgt ggtgagggaa
     7681 cgcggcgcgc gccaaccagc cacccctgtt ctaacaacat gcaggatctc cttatatgtg
     7741 gtgctaatac ccggttcaga gtagcatgtt tctaccttat gattcgttag ctatctggag
     7801 ttttaacatg ccgcctcgcc gctataaccc tgacacccga cgtgacgagc tgctggaacg
     7861 cattaatctc gatatccccg gcgcggtggc ccaggcgctg cgggaagatt taggcggaac
     7921 agtcgatgcc aacaatgaca ttacggcaaa acttttaccg gaaaactctc gctctcatgc
     7981 cacggtgagc acccgcgaga atggcgtatt ttgcggcaaa cgctgggttg aagaggtgtt
     8041 tattcaactg gcaggcgacg atgtcaccat aatctggcat gtggatgacg gcgatgtcat
     8101 caacgccaat caacccttgt tcgaacttga aggcccatcc cgcgtgctgt taacgggcga
     8161 acgcactgcg cttaattttg tgcaaaccct ttcaggagtt gccagtaagg tacgccacta
     8221 tgtcgaatta ctggaaggca ccaacacgca gttgttggat acgcgcaaaa ccttacctgg
     8281 cctgcgttca gctctgaaat acgcggtact ttgcggcggc ggagcgaatc accgtctggg
     8341 gctttctgat gccttcctga tcaaagaaaa tcatattatt gcctccggct cagtgcgcca
     8401 ggccgtcgaa aaagcgtcct ggctgcaccc ggatgcgcca gtagaagtcg aagtagagaa
     8461 tctggaagaa cttgatgaag ccctgaaagc aggagccgat atcatcatgc tggataactt
     8521 cgaaacagaa cagatgcgcg aagccgtcaa acgcaccaac ggcaaggcgc tactggaagt
     8581 gtctggcaac gtcactgaca aaacactgcg cgaatttgcc gaaacgggcg tggactttat
     8641 ctccgtcggt gcgctaacta aacacgtaca agcactcgac ctttcaatgc gttttcgcta
     8701 attgctgaca gatttatccg ctccatcgcg ggcggataat ctccccttcc ccaactttct
     8761 tcgtaacgcc tcgcaaattt cttgtctttt gctgcaacga aataaaaaac ctcggaagct
     8821 gctttcccgt ttcgtttttt gcccctcgct tgtgctgatg gcgtttgcca aagtagcgcc
     8881 aaccaaatca aggagcgaaa cagatggaca agcaacgcgg ttttacactt atcgaactga
     8941 tggtggttat tggcatcatt gccattttaa gcgccattgg tattcccgct tatcaaaact
     9001 acctgcgcaa agctgcactc accgacatgc tacaaacctt tgtgccttac cgtacagccg
     9061 tagagttgtg cgcgctggaa catggtggat tagatacctg cgacggtggc agcaatggca
     9121 ttccctcgcc taccaccacc cgctatgttt cagccatgag tgtggcaaaa ggcgtggtgt
     9181 cgctgaccgg gcaagaaagt ctcaatggac taagcgtcgt catgacaccg ggttgggata
     9241 acgcaaacgg cgtcaccggc tgggcgcgca actgcaatat tcaaagtgac agcgcattgc
     9301 agcaagcctg cgaagatgtc ttccgctttg atgacgccaa ctaaggagcg gcaatgaata
     9361 ttccacagct caccgccctg tgtctgcgtt atcagggagt cttgctggat gccagcgaag
     9421 aggtggttca tgttgcggta gtcgatgcac cttcgcatga gttgctggac gcattgcatt
     9481 tcgctaccac caaacgtatt gagatcacct gctggacgcg ccaacaaatg gaaggccacg
     9541 ccagtcgcac acaacagaca ttgcccgtag ctgttcagga gaagcatcag cccaaagcag
     9601 agttgctgac tcgaacgtta caatctgcgc tggaacaacg tgcgtctgat attcatatcg
     9661 aaccagcgga caatgcctac cgcatccgct tgcgtatcga cggcgtattg catcctttac
     9721 cggatgtttc accggatgcc ggagtcgcat taaccgccag attaaaagtg ctgggaaacc
     9781 tggatattgc ggaacatcgc ctgccgcagg acgggcaatt cactgtcgaa ctggcaggaa
     9841 acgccgtctc atttcgtatt gcgaccttac catgtcgggg tggtgaaaag gtggtattaa
     9901 ggttgttaca gcaggtgaac caggcactgg atgttaacac gctgggaatg cagccgttac
     9961 aactggcgga ctttgctcat gccttgcaac aaccacaggg actggtgctg gtaactggcc
    10021 ctacaggcag cggcaaaacg gtcacgcttt atagtgccct gcaaaagctg aataccgctg
    10081 acattaatat ttgtagcgtc gaagatccgg ttgagatccc catagccgga ctaaaccaga
    10141 cgcaaatcca tccgcgtgcc gggctcacct ttcagggcgt tttgcgtgcg ttattgcgcc
    10201 aggatcctga cgtcatcatg atcggagaga tccgctatgg ctttaacggc agaaattgcc
    10261 attaaagccg cgcaaaccgg tcacctggtg ttgtctaccc tacacactaa ttccacctgc
    10321 gaaacgctgg tacgtttaca gcaaatggga gtcgcccgct ggatgctctc atcagcgctt
    10381 acgctggtaa tagcccagcg tctggtacgt aaactttgcc cacattgtcg ccagcagcaa
    10441 ggggagccca tccatattcc agtcaatgta tggccgtcgc cgctgcccca ctggcaggca
    10501 cccggttgtg tacattgcta ccacggtttt tatggtcgta cggccttatt tgaagttctg
    10561 cccataacgc cggtcattcg tcagcttatt tccgctaata ccgacgttga atcgctggaa
    10621 acgcacgcac gacaggcggg tatgcgtacg ctttttgaaa acggctgcct ggccgtggag
    10681 caaggcttaa ccacctttga agagttaatc cgcgtactgg ggatgccgca tggcgagtaa
    10741 gcaactctgg cgctggcatg gcataaccgg cgacggcaat gcgcaagatg ggatgctatg
    10801 agcagagagc cgtgctttgc tgctcatggc actacagcaa cagatggtta ccccacttag
    10861 cctgaagcga atcgccatca attctgcgca gtggcgagga gataaaagcg cggaagtcat
    10921 tcatcaactg gcgacgctac tcaaagccgg gttaacgctt tctgaagggc tggcactgct
    10981 ggcggaacag catcccagta agcaatggca agcgttgctg caatcgctgg cgcacgatct
    11041 cgaacagggc attgcttttt ccaatgcctt attaccctgg tcagaggtat ttccgccact
    11101 ctatcaggcg atgatccgca cgggtgaact gaccggtaag ctggatgaat gctgctttga
    11161 actggcgcgt cagcaaaaag cccagcgtca gttgaccgac aaagtgaaat cagcgttacg
    11221 ttatcccatc atcattttag cgatggcaat catggtggtt gtggcaatgc tgcattttgt
    11281 tctgccggag tttgccgcta tctataagac cttcaacacc ccactaccgg cactaacgca
    11341 ggggatcatg acgctggcag actttagtgg cgaatggagc tggctgctgg tgttgttcgg
    11401 ctttctgctg gcgatagcca ataagttgct gatgcgccga ccgacctggc ttatagtgcg
    11461 gcagaaattg ctgttacgca tcccgattat gggttcactg atgcggggac aaaaactcac
    11521 gcagatcttt acgattctgg cgctgacaca aagtgcaggc attacttttt tgcagggcgt
    11581 agagagcgtc agagaaacaa tgcgctgccc gtactgggtg caacttctga cacaaatcca
    11641 gcacgatatc agtaacggtc atcccatctg gctggcgcta aaaaataccg gggagtttag
    11701 cccgctctgt ttgcaattag tgagaacagg agaggcatcc ggctcgctgg acctcatgtt
    11761 agacaacctc gcccatcatc atcgggataa cacaatggcg ctggcggata acctcgcagc
    11821 cttactggaa ccgacgttgc tgatcataac gggaggaatt atcggtacgc tggtggtggc
    11881 gatgtatctg ccaattttcc atttaggcga tgcgatgagt gggatgggat aacgctggcg
    11941 tgttgctcca caccagcgtt ggaagattac aggttgttga agatgcggtt ttcttgttcc
    12001 tgcacacgga taaatgtggt gcgcttggtc agttctttca ggcgtgaagc cccaacgtat
    12061 gtacaagctg aacgcaggcc gcccaaaata tcacgcgcgg tattttcaac cgggcctcgc
    12121 agcggcagct taacggtttt accttctgct gcgcgatatt ccgcaacgcc gccaacgtga
    12181 cgtttcatcg cagactcgga gctcatgccg tagaacagca taaatttctc gccgttctcc
    12241 tcaacgatgc gaccgccgct ctcttcgtgg cccgccagca tgccgccaag catgacgaaa
    12301 tcggcaccgc cgccaaaggc ttttgcgaca tcgcccggcg tggtgcagcc accgtcgctg
    12361 acgatcattc cgcccagacc gtgcgccgca tcggcacatt cgattaccgc agaaagctgc
    12421 ggataaccga cgcctgtttt gacgcgagtt gtacaaacag aacctgggcc aatgccaact
    12481 ttaacgatat cagcaccgga gaggataagc tcctcacaca tttcaccagt cactacgttg
    12541 ccagcacaaa tggttttggt cggccacgct tcacgcgctt tcgcaacgaa ctgcacgaag
    12601 tgttcggaat aaccattcgc cacatcaata caaacgaagt ttaatgccgg gttcaggtcg
    12661 agaatctgtt tagttttttc gaaatccgca tcagacgtac cggtagaaac catcacatgt
    12721 ttcagcacat cagcggaaga attgttgata aacgcttgcc actcttcgac agaatagtgt
    12781 ttatgcacag cagtcaaaat atcaaaagaa gccagcgcag aggccataga aaatgtacct
    12841 acggtgtcca tatttgcggc gataatcggc acgccggacc agctctgacc tgaatgtttg
    12901 aaggtgaatt gacgttccag ttcaacatcg gaacggcttt taagagtgga gcgtttaggg
    12961 cggatgagaa cgtctttaaa acctaacttc agatcttctt caatacgcat gtgcggattc
    13021 ctggggttaa tggctataag ggtaagagcg caactccagt gacgctatca tacgcactaa
    13081 tcaatgcggc gcaagactgc gaaattgtct ttttttacgc tacaatccca taaatttacc
    13141 tggtaaatag tgagcgaacg tttcatcaat ttcgctttcg tgcactcatt tttaacgttt
    13201 tgataatcat gatgaattcc cgggaatata aattatgagg tatatagttg ccttaacggg
    13261 aggcattggc agtggcaaga gtaccgttgc caatgcgttt gctgatctcg gaattaacgt
    13321 cattgatgcc gatattattg cgcgtcaggt ggttgaacca ggtgcacctg cgctacatgc
    13381 cattgctgat cactttggcg ctaacatgat tgctgctgat ggaacattgc agcgccgggc
    13441 cttgcgcgag cggatcttcg ccaacccgga agagaaaaac tggcttaacg ccctgctgca
    13501 tccgctgatt cagcaagaga cgcaacacca gatccagcaa gctacttccc cctatgtact
    13561 gtgggttgtg ccattgctgg tagaaaactc actgtataaa aaagcgaatc gagtgctggt
    13621 ggtggatgtc agcccagaaa cgcaacttaa gcgcaccatg cagcgcgatg atgtaactcg
    13681 cgagcatgtc gaacaaatcc ttgctgctca ggcaacgcgc gaagcccgcc ttgccgtggc
    13741 agatgacgtc attgataata acggcgcacc ggatgctatc gcatcggatg ttgcccgcct
    13801 gcacgcacac tatttgcagc ttgcgtcgca gtttgtctca caggaaaaac cgtaatgcag
    13861 acccaggtcc tttttgaaca tccactaaat gaaaaaatgc gtacatggct gcgcattgag
    13921 tttttgattc agcaactcac cgttaattta cccatcgttg accacgctgg cgcgctgcat
    13981 ttcttccgta atgtcagtga attactggat gttttcgagc gcggcgaagt ccgcactgag
    14041 ctgttgaaag aacttgaccg gcagcaacgt aaactccaga cctggattgg cgtgcctggc
    14101 gtggaccaga gccgtattga agcattaatt cagcagttaa aagcggcggg gagcgtatta
    14161 atttccgcgc cgcgtatcgg gcaatttctg cgtgaagatc gtttgattgc tctggtgcgt
    14221 cagcgactga gcatcccagg cggctgttgc agctttgatt tacctacatt gcacatttgg
    14281 ctgcatctac cccaggcgca gcgcgacagc caggtagaaa cctggattgc cagcctgaac
    14341 ccgctcaccc aggcacttac catggtgctg gatttaattc gccagtcggc ccccttccgt
    14401 aaacaaacca gtctgaatgg tttttatcag gataacggtg gcgatgccga cttgctgcgc
    14461 ctgaatctgt cgctcgattc acagctttat ccgcaaattt ccggacataa gagccgtttt
    14521 gccattcgtt ttatgccgct ggacactgaa aacggacagg taccggaacg tctggatttc
    14581 gaactggcct gttgctaagg agtaaaaatg tcagaaacta ttacggtgaa ttgcccaacc
    14641 tgcgggaaaa cggtggtgtg gggtgaaatc agcccgtttc ggccattttg ctccaaacgt
    14701 tgtcagctga tcgacctcgg agaatgggct gctgaagaaa aacgaatccc cagcagtggc
    14761 gatctttccg aaagcgatga ctggagcgaa gagccaaagc agtgacattt gtctgatgcc
    14821 gcacgtaggc ctgataagac gcggacagcg tcgcatcagg catcttgtgc aaatgtcgga
    14881 tgcggcgtga acgccttatc cgacctacag acgcttaagc ttcgcaatta ccggttcatt
    14941 ggctggcgga aaatcatcgg cattaagacc gactagcgac atccactcac cgggttgccc
    15001 ttctttaccc cacggctccc cttcccagct ttcgaccagc cagaaccaca gagttatatg
    15061 cctgtccggg aattcatatt ccagtttttc aaatagcgaa aaatgttgtg gggtaatccc
    15121 gacttcttcc tgaagttcac gcactaccgc ctgttccggc gtttcgccca tttcaatttt
    15181 accgccggga aactccagtt tattcgccat gtgcgcatct gctgcgcgac gcgttataaa
    15241 gatttcattg ttctcgttgc gaataatacc taccgcaatt tgcagctttt tcattgtctt
    15301 aaacctataa aaaaggcgca gaatcctgcg ccttttactt caactgttag cttttattgc
    15361 aggcgaccat ggcactgctt gtatttttta ccagaaccgc acgggcaagg atcgttacgt
    15421 cctactttgc gctcaccggt ttgcgccgcc agtgcagctg cggctgcaga gtcgtcatcc
    15481 tgatggctaa gctgctgcat ttgcgctaaa cgctcggctt ccatacgacg ctgttgttcc
    15541 agctcctcaa cctcttcagg catacgtacc tggactttgc tcagcgtgct gataacttca
    15601 tattttaacg actccagcat cgctgcaaac atggagaacg attcacgttt gtattcctgc
    15661 ttcggatctt tctgtgcata gccacgcagg tggataccct gacgcagata gtccatcgcc
    15721 gctaggtgct ctttccacag agagtcgaga gtttgcagca tgacgccttt ttcgaagtga
    15781 cgcatcatct cagcaccaac cacttcttct ttacgctgat acacttcgat ggactgcgcc
    15841 agaattcgct cacgcagcgt ctcttcatgc agttctggtt ctttatccag ccactcggca
    15901 attggcaaat cgaggtcgaa atcgttcttc agacgttcct gcagccccgg aatatcccac
    15961 atttcttcca gtgactgtgg cggaatgtag gcatcaatgg tcgctttgaa cacatcttca
    16021 cgaatgctgt tgatggtttc gctcacatcg ctgacatcca gcagttcgtt acgctgggag
    16081 taaatggcgc gacgctgatc gttagccacg tcatcatatt ccagcagttg cttacgaatg
    16141 tcgaagttac ggctttcaac tttacgctgg gcgttggcaa tcgctttagt cacccacggg
    16201 tgttcaatgg cttcgcctgg cttcataccc agtttacgca tcatgccgga tactcggtcg
    16261 gaagcaaaaa tacgcatcag cgcatcttcc atcgacaggt agaaacggga agaaccagca
    16321 tccccctgac gaccagaacg accgcgcaac tggttatcga tacgacggga ttcgtgacgc
    16381 tcggtaccga tgatatgcag gccacctgct tccagtaccg catcgtgacg tacctgccag
    16441 tcggctttaa ttttttcaat ttgctctgcg gtcggatttt ccagcgcggc aacttctgcc
    16501 tgccagctac caccgagcac aatatctgta ccacgacccg ccatattggt cgcgatagtc
    16561 accgcagccg gataacctgc ctgagcaaca atcgccgctt cgttggcgtg gaatttggcg
    16621 ttcaggacgt tgtgcttaat accggctttg gtcagttcgt ttgacaccag ctccgatttt
    16681 tcgatggaga tagtacccac cagcaccggc tggcctttcg cagtacgttc tttgatatct
    16741 tcaatgatcg cctgaatttt ttccgcttca gtcatgtaga ccaggtccgg cagatcttta
    16801 cgaatcattg gacggttggt cggaacaacg acggtatcca gcttgtagat ggagctaaat
    16861 tcgaaagctt cggtatcagc agtaccggtc atccccgcca gtttttcata cagacggaag
    16921 tagttctgga aggtgatcga agccagcgtt tggttttcgt tctggatctg cacaccttct
    16981 ttcgcttcca cagcctggtg cagaccatcg gaccagcgac ggccctgcat ggtacgaccg
    17041 gtgtgttcgt caacgatgat aacttcacca tctttaacga tgtagtcgac gtcgcgggta
    17101 aacagcgcat gagcgcgcag cgccgccgtt acgtggtgca tcagcatgat gttggccgga
    17161 gagtacagag actccccttc atccatgatg ccctctttca ccagcagttc ttcaatcagc
    17221 accagaccac gttcggtcag gttcacctgg cgagattttt cgtccaccga gaagtggcct
    17281 tcgccctgga aggtttcgga gtcttctttt tcctgacgga tcaggtgcgg aataatttta
    17341 ttcacgcgtt tatacatttc cgagctgtct tctgccgggc cggaaatgat cagcggtgta
    17401 cgcgcttcat cgatcaggat ggagtccact tcgtccacca gcgcatagtg cagtttacgc
    17461 tgtacacgtt cttcagggct gaacgccata ttgtcgcgca ggtagtcaaa gccgtattcg
    17521 ttgttcgtac cgtaagtgat gtcagctgcg taagcttcgc gctttgccgg tgctggcatg
    17581 cccggcaggt tgataccgac agtcaggcca aggaattcaa acagcggacg gttgttttcg
    17641 gcgtcacgtt gcgccaggta gtcgttgacg gtaactacgt gcacgccttt accggttagt
    17701 gcgttcaggt aagcaggcag cgttgcggtc agggttttac cttcaccggt acgcatttcg
    17761 gcgatgcagc gttcgttaag aaccataccg ccgagtaact gaacgtcgaa gtgacgcata
    17821 ccaaagacgc gcttacttgc ctcacgtacc acggcgaaag cttccgggat cagattttcc
    17881 agcacttcgc ctttttccag acgtgcacga aactctgcgg ttttcccttt cagttcttcg
    17941 tcggagagtt tttccatctc cggttccatg gcattgatga tgttgaccac tttgcgcatc
    18001 cggcgcaggg tgcgatcgtt acgactaccg aaaactttag ttaacaattt gattagcata
    18061 ataaaatctc aaacgccccg cgttgcggag ttaataaaat gaagtaaagg tttattgttg
    18121 ttaggtgagg cgttgagggc cagcacggat gccttgcgcc tggcttatcc agacgggcgt
    18181 gctgaatttt gcttgtgggg taaaatgcgc ataatcaatg cgataaccct tttcagacgg
    18241 cgtgccttcc tgggtcagca gcgcgctgag cgtatccagt aatgcaagat gttgcgcctg
    18301 aagaggcaaa gactcttcag caacgggcag tgtttgcggt gccattgcga aagaaagatg
    18361 acggattacc gtgcgaatgg catgttgatg ccagtaatca acggaatagt tcgaattcgg
    18421 gcggcgtgtg ttcgcttcca gcaaggccaa ttgaccaaag ttaactttgg ctgaaggctc
    18481 gtggttgcgg gttgtcgctt ttgcgggcgc gtttggttcg gcggcgttgc tgagcgcagg
    18541 caaacctaaa ctcgccgcaa ccatccctaa taagagatgc ggccagaagt agcgtttacc
    18601 aaactgtcgc cagcgcgtca gtattccact cacgttattg ccatcccgat cgtattcaaa
    18661 cgtatttagc gcacaaatct tatcattaaa gcctgcagtc cacagcacga atgaagataa
    18721 gttgtgcgga aaagtgctta agaaaacagc gttcgcaccg ttttttatag ctcttataaa
    18781 attcaaaaaa aaatgactgg ttcacctggc cggagagagt gccagatttg ccagtcgaat
    18841 tttatacgac agtataaatg tcgttatgcc agtacagctg aaggcgcttt gaaggccaac
    18901 ggcagttctg cgtcgtcctg gaaggtcaca tattcccagg cttcctgttt cgccaggaca
    18961 gcctgcagca gtttgttatt cagtgcatga ccggatttat aagcggtaaa tgcaccaata
    19021 atattgtgac cacacatgaa caagtcaccg atcgcatcga gcattttgtg acgcacaaat
    19081 tcgtcttcaa aacgcaggcc gtcttcgttc agtacgcgat aatcgtcaac aacgatggca
    19141 caatcgaagc tgccgcccag gcacaaacca cgggactgca gatattcgat atcacgcatg
    19201 aaaccgaacg tacgcgcacg gctgatctgg cgcataaacg catcagcgga gaagttcatc
    19261 gcatagcgct ggttgctgga atcaatagcc ggatggttaa aatcgatggt gaaatccagc
    19321 gaaaaaccat tgtacggctt aaattcagcc cacttatcgc catcttcgac acgaacagtc
    19381 tctttgatgc gaacaaattt tttggcgcag ttcaactcgt cgataccggc gtcaagcagc
    19441 aggtatacaa acggagcggc gctgccgtcc atgatcggga tttccggcgc gttaacttcg
    19501 ataacaatgt tatcgatgcc caggcccgcg agagcagcat tgaggtgctc tacggttgaa
    19561 atccgtacat catgctcgtt gaccagacac gtacagagca tggtatcacg cacagatttg
    19621 gcatcggccg ggaaatctac cggtggattc aagtcggtgc gacgatagat gaccccggtg
    19681 ttggccggcg cagggcgtaa cgtcagagtg actttcttgc cggtatgtaa accgacaccc
    19741 gtcgcctgaa cgatacgttt aagtgtcctt tgtttgatca tcgtattatc tcgccaaatt
    19801 acctatccaa ccgaagtgta ctatacattc ggcgggccag tttagcacaa agagcctcga
    19861 aacccaaatt ccagtcaatt cttaatcagc ttgcttacgc aggaatgctg ggatatccag
    19921 ataatccggc tctttcgcag tttgcggcgc attgtcattc acgactttag caaccggctt
    19981 ctgctcctgg gtcagcggag ccatcccatg ctgctggtag cgatccatca ctggctgctg
    20041 aacctgctta ttggtcacca gagtgatttc aggacgtttg tccatgccga tacctgtcgc
    20101 aacaacggtt acgcgcagct cgtcattcat atccgggtca agagaagtac cgataaccac
    20161 agtcgcgttg tcggaagcaa atgcacggat ggtgttacct accgtttcga actcatccag
    20221 acgcaggtcg aagcccgccg tgatgttaac cagcacgccg cgcgcgccag acaggtcgat
    20281 atcttccagc agcggagaag agatagccat ttcagcagct tcttccgcac ggtcttcacc
    20341 gctcgccacg ccagaaccca tcattgcgta gcccatctca gacattacgg tgcgtacgtc
    20401 tgcaaagtcc acgttcatca aacccggacg agtaatcagt tcagcgatac cttgcacagc
    20461 gcctttcagt acatcgttcg ctgcgccaaa cgcatccagc agggagatac cgcggcccag
    20521 aactttcagc agtttgtcgt tcgggatagt gatcagagag tccacatgct tggacagttc
    20581 agtgatcccc tgctccgcga atgccatacg cttcttgcct tcaaagttga aaggcttagt
    20641 gacgacagca acggtcagga tacccaaatc ttttgccact tcagcgacga ctggtgctgc
    20701 acctgtaccg gtaccaccac ccatacccgc agcaataaag accatgtctg caccttccag
    20761 cgccgcacgc aatgcatcgc gatcctcatc agccgcattg cgaccaactt ctggattagc
    20821 gccagcgccc agtcctttgg tgataccgct accgatttga atcgtctgtc caaccgctgt
    20881 tttacgcagc gcttgtgcat cggtatttac cgcgaagaat tcaacacctt caatacgctc
    20941 gcgcaccatg tgttcaacag cattaccgcc gccgccgccg acgccgatga ctttaatcac
    21001 cgcgtcattg gtaagttcca ttggttcaaa catagtttct ctccgatttg tgcctgtcgc
    21061 ctgaggccgt aatcatcgtc ggcctcataa aaattaaaac tcttttcgca gccaactatt
    21121 gagtcgcttg atccacgagc caactgatgc tgtaacacgt ttttctactt cagcttcacc
    21181 gttaagatgt gactctttcc catagtgaag caatcccacc gccgtcgaat aatacggctc
    21241 ctgagcataa tccgttaaac cggtaatgtt cagcggcgcg ccgatacgca cttgcgtatg
    21301 aaacacgcgc tgagcacagg ctgcaagacc ttcgatctgc gctgcgccac cggttaatac
    21361 aatgcctgcc gccaggtgat gtttaacccc ttgttggcga agcttttcct gcaactgcaa
    21421 tatctcttcg ttgaccaggt tgagcagctc ggtatagcgc ggctcgatca cctctgccag
    21481 ggtctgacgt tgcagactcc gtggcggacg accgcctacg ctcggcactt ccacgctctc
    21541 atcttttcca acgatggaac ccagcgcaca accgtggcga actttaatcg cttcggcgtc
    21601 gcttggcggc gtgccaaagg cgtaagcgat atcactggtc acgacattgc cagcataagg
    21661 aattacctta gtgtggcgca atgccccacc ggtataaacg gcgatatcca ttgtaccacc
    21721 accgatatcg acgacgcaga cacccagttc acgttcatct tccgtcaata ccgaataact
    21781 tgatgccagt ccggcaaata tcagttggtc aactttcagc ccacaacgtt caaccgcttt
    21841 gacgatgttt ttcgccatat cgttgtgaca tgtgatcagg tgcacttttg cctgcatccg
    21901 cacgcccgaa agtcctaccg gattcttgat cccttcctga tagtcaatcg catactcttg
    21961 cgggatcaca tgcagcacac gatgttcatc gcgcacacgc accgatttcg cggtatggac
    22021 gacgttttcc acatcttctt gcgtcacttc ttcttcagaa ataggcacca taccaatttc
    22081 attctggcag ctgatgtgct taccagaaag cgccagatat accgaagaga tctgacaatc
    22141 tgccatcaat tctgcctggt caatggcgcg ttgtacgcac ttgaccacgg attcgaggtc
    22201 gttcaccccg cctttatcca taccacgtga cgggcagctg cccacgccaa tgatattgac
    22261 cataccgtcg ggcagaactt cccctactaa agcggcaacc ttcgcggtac caatctccag
    22321 tcctactacc agttttctgt ccgtcgcctt gatcattgtt gttctgcctg tgcctgattt
    22381 tgttgctgag tagattcctc tggcggcaag ggcgcccagc ctactgccgc tccagagtca
    22441 taacgcaaat caacgtagct aatccgtttg ccatcggttt gcgcctgctg ctgtaaaacc
    22501 ggataaagtt ctacaaagcg agccaaacgt ttcatcgtat cgccccggcc aagattgagc
    22561 ttaatatcgt tattcagcgt caactgccag gaacgccgcg cggtcatcgc cgcttccttc
    22621 agagtaaatc tgtcctttgc cagcatctgc cccatttcgc gatagccctg caacacttca
    22681 ttggcgctgc cttccgggcc atacagcatt ggaagcacct gcttgctggt gcgatctggc
    22741 ggcacgctga aggtgtttcc ttccgcgtct accatatgtt gatcattcca ccgcgcaatc
    22801 ggcacatatt caaccagatg aatcttcaat tcatcaggcc actgctttct gacgctcacc
    22861 tgcttaatcc acggcaggcg ttgttctatt tgcgtctgga tgatgttgac atcctgggtc
    22921 ataaaggtac ccggctcacc caatgccagg atcgactgcc ggatatcgtc attacgcgtg
    22981 taatggcgtt caccggtcaa caccagcttt gagagcggca ggcgttgcgc atcttccatc
    23041 cagcccaaca cgacccagcc gctcaccaac actgtcgtta aaacggtcag caggaaaagg
    23101 atccccgcca gacgcgttcc attattgcgg cgagaagaaa cctcttcttc gctatttcgc
    23161 gtgttcagag cagcctgcga catattagtc cgccagttcc agaattcgta ctaccaactg
    23221 cgagaagctc atacctgcct gacgtgccgc catcggcacc aggctgtggc tggtcatacc
    23281 cggtgaggta ttggcttcca gcagataaaa ctgtccatcg ctgtccagca taacgtcaat
    23341 acgtccccat cctttgcaac ctaacgtcgt ccatgctttc agcactaatg cctgcaaatt
    23401 ggcctcttgt gacgcttcca gacctgcggg gcagaaatac tgtgtctcat cagagagata
    23461 cttcgcctca taatcataga aggttccgga cggttgaata cgtattgacg gtaaaatttc
    23521 ttcaccgagt atcgcaaccg tgaactccgg cccacttagc catttttcaa tcaatacttc
    23581 ttcatcgtgc tgaaatgcca atcttaatgc atcttgtaga gcattttctg ctactacttt
    23641 tgacattccc acactggaac cttcgcggct cggcttaacg ataaccggca aacccagagc
    23701 agaaatttct gctaactgct tatcgctcag gcctttttca aactctgcgc gggttaacgc
    23761 tacccacggc gcgaccggta aaccggcacc ttgccacagc agtttgctgc gtagtttatc
    23821 cattgaaagc gcagatgcca tcactccgct gccggtataa ggcaagccca tcagctcaag
    23881 catgccctgc agcgtaccat cttcaccgcc gcgaccgtgt agcgcgataa acactttctg
    23941 aaaacccatc gacttcagtt gcgtcacgtc gacttctttc gggtcgacag gatacgcatc
    24001 aataccgcct tcacgcagcc cggctaacac cgctgcgcca gaattcagag aaacttcccg
    24061 ctcagcggaa gtcccgccca acaggaccgc aattttatca gtcatgttgt tcttcctccg
    24121 gagtttgcgg cttcagtttg atttcagcta aagaacgggc aatttttccg atattaccag
    24181 ccccctgaac gagaatcagg tcgttaccga ttaataccgg tgccagcatc tcggctacct
    24241 gcgcagggtc cggcaccaga atgggatcaa ttttcccacg tccacgaatt gtgcgacaca
    24301 gcgaacggct gtccgctccc ggaattggcg cttcgccagc cggatacact tccagcatca
    24361 acagggtatc aacctgcgtc agcacattgg cgaaatcatc atacaggtcg cgcgtacggg
    24421 taaaacggtg cggctgaaac agcattacta ggtttttatc cggccagcct gcgcgcgccg
    24481 ctttaatggt ggcgtcaact tccgtcgggt ggtggccgta gtcatcgacc agcattgccg
    24541 taccgctttt accattcact ggctccagcg ggaattcacc gaggaaatca aaacggcgac
    24601 cagtcccctg gaagctctcc agcgcacgca aaatagcctc gtcgtcaatt ccctcttccg
    24661 tagcaaccgc aaccgcagct gcggcgttca gcgcgttatg acggcctggc gcattcaggg
    24721 tgacgcgcat cggctctttg tcctggcgca gcagcgtaaa gtgcccctgc gggccaatct
    24781 gctgataatc ttctacacgc acgtcggcat cttcgctgaa gccgtaagtc gtggtctgac
    24841 gtcccacacg cggtaacaat tcgcggatca ccggatcatc aacacacatc accgcacgac
    24901 cgtaaaacgg caggttgtgc agaaaattaa taaaagtctg ttttaaattc tcaaagtcgc
    24961 cctggtaggt atccatgtgg tcggcttcga tattggtgac aatcgccacc atcggttgca
    25021 gatgcaggaa cgatgcatca ctctcatctg cttcggcaat caggtaccga ccatgcccca
    25081 aacgcgcatg aacccccgcc gcttttacca gcccgccgtt aacgaaggtt gggtcgagcc
    25141 ccgcttctgc gtagatgctg gaaaccatcg cggtggttgt cgttttgccg tgcgttcctg
    25201 caatggcgat gccatgacga aaacgcatta actcagccag catttcggca cgacggatca
    25261 ccggaatacg cgcttcatga gctgcgacaa tttccgggtt atcggcagaa atggcgctgg
    25321 aaacaacgac cacgctggca tcacgtacgt tttccgggcg atggttgaaa taaatcgtcg
    25381 ctcccagatt cattaactgc tgcgtgaccg gatttggcgc taaatcggaa ccactgatct
    25441 gataaccttc attggccaga acttcggcaa taccgcccat accggcacca ccgatgccga
    25501 caaaatgtat gtgccgaacg cgacgcattt cgggcacgat ggaacgcagt tttgccaatt
    25561 gttgtgtatt cattctttac gccattaact tcttaaattc atacgatgca aaaggcatcg
    25621 ctacaattac gcccgggcag cccggctcac ttcatttgcc actcgctcgg tggcatccgg
    25681 aatggatgca gcgcgggcgc gttctgccat ggttaataag gtttctcgcg accacccggc
    25741 cagggtgttg gcgacagcat ccacgctaag ctgtggctgc tcgataattt tggctgcgcc
    25801 cgctttttcc agcggtagcg cattccagta ttgctggcgg tctttatgtt gaaacggcac
    25861 aaacaacgcc ggtagtcctg ccgcggcgat ttcactcacc gttaaagcac cggagcggca
    25921 aacgacgaca tccgcccacg catacgccgc cgccatatca tcaataaatt ccgtcacttt
    25981 atgctgcggt tgccccgctt cggcatacgc ctgttcaacg gattgttgcg aacctttacc
    26041 gctctgatgc cagatagtga ctgaatcacc cagtttcgca gcaacctgcg gcattgtctg
    26101 gttaagaatg cgtgcgccct gggaaccacc cactaccagc acacgaaccg gaccttcacg
    26161 tccagccaaa cgttgctgcg gcaacggcag cgccagcaca tcggtacgca ccgggttacc
    26221 caccacttcc gcattaggga aagcgcctgg aaacgcctgc atcactttgg tggcaatctt
    26281 cgccagccat ttattggtta agcccgcaat accgttttgt tcatgaagta caaccggaat
    26341 gcctaacgac cacgcggcca gaccacctgg acctgataca tagccgccca taccgagtac
    26401 cacgtcaggt ttgtacgctt tcataatcgc ccgcgcctga cgccaggcgt tgaagatacg
    26461 cagcggcgca gctatcagtg cttttatacc ttttccacgc agaccagaga tacgaatgaa
    26521 atcaatttcg atgccatgtt ttggcactaa gtccgcttcc atacggtcgg cagtccccag
    26581 ccagcgaact tgccaaccct gagccattag atggtgcgca accgccagtc ccgggaatac
    26641 atgtccaccg gttccgcctg ccatcaccat taatcgcttt ccttgaccac tcatcgtgaa
    26701 cctcgtacaa acgcctgcgc tttctccaga cgcgtttcat aatcaatacg caacagcatc
    26761 atgatggctg tcgacataat cagtaagctc gaaccaccgt aactgatcag cggcaatgtc
    26821 agacctttgg tcggtaacat ccccgccgcc gcgcctacgt taaccagcgc ctggaagcta
    26881 aaccagatgc caatagaaca ggcgagaaaa ccggaaaaac ggtggtcaat ttctaacgct
    26941 ttgcggccaa tcgacatcgc gcgaaaagcg acgaagaata ccattaaaag tgccagcacc
    27001 acaccgacat accccagttc ttcgccgata atggcgaaaa taaagtcagt gtgcgcttcc
    27061 ggcagatact ccagtttttg taccgagtta cctaaacctt gtccccaaag ttcgccgcga
    27121 ccaaacgcca tcagcgattg cgttaactga tagccgctgc caaagggatc ttcccacggg
    27181 ttccagaatg cggtaacacg gcggatacgg tacggttcgg cgagtatcag caacacaacc
    27241 gctgaaatgc ccataccgat aatggcaatg aactgccaca atttcgctcc cgccaggaac
    27301 aacatcgcca gcgtagtcac aaacaacacc accaccgtac caaggtctgg ctgtgccagc
    27361 agtaacactg ccaacaccag aatcacgccc atcggtttca ggaagccgcg caggttatta
    27421 cgtacttcgt cgcctttacg caccagatag ttggcgatat aacaaaacag cgacagtttt
    27481 gtcagctccg caggctggat acgcagcaaa ccgagatcga tccaacgcga tgccccttta
    27541 accgagctac ccactaccag cacgatcatc agcaggatga tagatccgag cagcatcgtg
    27601 gcactgtagc gttgccagaa ctccatcggc agacgcagcg taatgatcgc cagaatgaat
    27661 gccaggatca gatagacgcc atcacgcttc gcgaagaaga acggatcgtt ggttaagcgt
    27721 tgccctatgg gcattgacgc cgaggtcacc atgataaagc caatcgccgc aaggccgaag
    27781 gtcagccaca gtaaggtgcg atcgtacatg atcaggctgt cggtatcttt ttcccgcgag
    27841 cccatcaccc agccttttag cgccgtggag atccagacca ggatactgaa tcctggcagg
    27901 cgcggcattt tcaggcgagg gagagataaa cgcatcaacc taactccttc gccagacggg
    27961 caaactcatt gcctcgttgt tcaaagttct tgaactgatc aaggctggca caggccgggg
    28021 agagcagaac catatcgccc agctgaacac gcggagccag caagcgcatc gcttgttcca
    28081 tggtttcggt ttgttctgcc acttccgggc gtagcgccgc cagctgcgca ccgtcacgac
    28141 caaaacagta caaacgcacg ttgtcgccat tcagatagcg cgccagtgga ctgaagtccg
    28201 ccgatttacc atcgccgccc agcaacaaat gcagcgtgcc gtctacctgc aagccattca
    28261 acgccgcttc cgtactgccg acgttagtgg ctttcgaatc gttaatccag cgtacgccat
    28321 tgtgctccag caccacttca aagcgatgcg gcagaccggt gaaggtggtt aacgctttca
    28381 ggctgctggc gcgcggcaaa ccagctgcgt ctgccagtgc cagtgctgcc agcgcattgg
    28441 tgtagttatg ctgcccggaa agtttcatct ctttcacatt cagcactttc tcgcctttaa
    28501 cccgcagcca ggtttcgccc tgctgatgat tcaggtgata gtcacccatg ttgacgccaa
    28561 agctgacgca gcgttcatca gcaccgcgaa tcggcattgt taaggcatca tcagcattga
    28621 cgacacaaac tttcgcattt tcgtaaatac gcaattttgc cgcacggtat tgctgcaaac
    28681 caaacggata gcggtccata tgatcttcag tcacgttcag aatggtcgcc gccaccgcct
    28741 gtaagctgga agtggtttcc agctggaagc tcgacagttc cagcacgtac agttcgcact
    28801 cagcatcaag cagcatcaac gctggcaggc caatattgcc acccacacca acgttaaccc
    28861 ccgccgcttt cgccatttca cccactagcg tggtgaccgt gcttttgccg ttagaaccgg
    28921 taatcgccac aatcggtgct tgtgcttcgc gacagaacag ctcgatatcg ccaacgattt
    28981 cgattccggc atcagcggca gcgcttaagg atggatgcgc ctgtgcaata ccgggactgg
    29041 cgacaatcag atctgccgcc atcagccatt catcattcag actgcccgtg tggcgttcta
    29101 cggcttcggg taatttatcc aggccaggcg gtgtcatacg cgtatccata acgcgcggcg
    29161 tcacaccgcg agcgaggaaa aagtccacgc aggaaagccc ggtgaggccc aggccgataa
    29221 tgacgacatt tttaccctga taatcagcca tgattaacgt accttcagcg ttgccagacc
    29281 aatcagaacc agcatcagcg aaataatcca gaaacgcaca atgacgcgcg gttccggcca
    29341 gcctttcagt tcatagtggt gatgaatcgg tgccatgcgg aaaatacgtt gtccgcgcag
    29401 tttaaaggag ccgacctgca ggatgacaga aagcgtttct accacgaaca cgccccccat
    29461 aatcaccagc aggaattcct gacgtagcag tacggcgata atgcctaacg caccacctaa
    29521 cgccagcgaa cctacatcgc ccataaagac ctgcgccgga taggtgttaa accacaggaa
    29581 gcccagtcct gccccgacta tcgccgtgca gacaatgacc agttcccctg cgtgtcgcag
    29641 atacggtata tgcaagtagc tggcaaagtt catattgccg gtcgcccacg ccaccagcgc
    29701 aaaaccaccg gcgacaaata cggtcggcat aattgccagg ccgtcgagac catcggtcag
    29761 gtttaccgcg ttgccagtac ccacaatgac gaagtaagcc agcagaatgt agaacagccc
    29821 cagctgcggc atcacatctt taaagaatgg gaccaccagc tgcgttgcgg gcgtgtcttt
    29881 gccggcaagg tacagggcga aggcgacacc cagcgcaatg accgacatcc agaaatactt
    29941 ccaacgagcg atcaaccctt tggtgtcttt acgcaccact ttgcgataat catcaacaaa
    30001 gccaataaca ccgtaaccta ccagcaccac caacacgcac cagacgtacg gattggacgg
    30061 gtaagcccac agcagtacgg agatcacaat cgccgtcagg atcataatcc cgcccatggt
    30121 cggcgtaccg cgcttgctga agtgtgattc aggaccgtcg ttacgcacca cctgaccaaa
    30181 ggaaagtttt tgcaaatgag ctatcatacg cgggcccatc cacaatgaga tgaacagcgc
    30241 ggtcagcagg ctgacgatgg cgcgaaacgt cagataggaa aagacgttaa agccggaata
    30301 atatttgacc aaatgttcgg ccagccaaac taacatgtcc cattctcctg taaagcgcgt
    30361 actacctctt ccatggcggc actacgtgaa cccttaacta aaatcgtaat tacctgttgc
    30421 tcagcaatca gtgatttaag acgcgtaatt aacgcagttt tatcagcaaa atgttcgcca
    30481 acgccgctgg cggtgctgat agcatggctt tgtttaccca cgcttaacac gcggtcaata
    30541 ccagcagctt ttgccgcctc gcccacctgt acatggcagg cttcgctttc agcgcccagt
    30601 tccgccatat cgcccaccac cagcacgcgg tagcccggca tttcagccag tacctgaaca
    30661 gcggcggtca ttgaaccgac attggcgttg taggagtcgt cgagcagcaa ctggttttct
    30721 gccagttgga tggggaacag acggcctgga acagctttca gatttgccag ccccgctttg
    30781 atagcatcaa gcgttgcgcc cacggacatg gagagcgcag cggctgccag cgcattcgca
    30841 atattgtgac gccccggcaa cggcagcaga acatcgacgc taccggttgg ggtttgtagg
    30901 gtaaattccg taccgtgcga ggtcacatgg atattggtgg cggtgaaatc gctgttggcg
    30961 gcattgggtg agaaacgcca cactttgcgt gagccaatta cgctctgcca gttcagccag
    31021 tcgttgttgt cggcgttcat aatggcgata ccgttttccg gcaggccgct aaagatttca
    31081 cctttcgctt tcgcgacacc cgcaagcgag ccaaaacctt ccagatgcgc cgctgccagg
    31141 ttgttgacca gcgcagcttc cgggcgagtc agactcacag tccaggctat ttcgccctga
    31201 tggttcgcgc caagttcaat aactgcgtaa tcgtattccg gcgtcaagcg caacaacgtc
    31261 atcggtacac cgatgtcgtt gttgagattg cctgccgtat aaagcgtgtt gccgcactgg
    31321 cttaaaatcg ccgccgtcat ctctttaacg gaggttttgc cggaggaacc tgtcagagca
    31381 accacgcgcg ccggaacttg ctggcgaacc catgcagcca gttcaccaaa cgccagacgc
    31441 gtatccttga cgattaactg cggcaggtcg atatccagcg gacggctgac cagtagtgcg
    31501 cccgcgccgc cagctttcgc ctggtcggca aaatcatgag catcgaaacg ttcgcctttc
    31561 agggcaacaa acaggcagcc cggcgtcagt tttcgggtat cagtggttac agcatcaagg
    31621 gtgatatctg caccttgcag ttcaccgttg agaatgtcgg taagttggct aagggttacg
    31681 ctaatcatgc aatcaccccc agcagacgcg ccaccgtgac gcgatcggag tagtccagac
    31741 gctgattgcc aacaatctgg taatcttcat ggcctttgcc cgcgaccagt accacatcat
    31801 tctctttagc ctgcataacg gcgcaagtca ccgcttcagc acggccttcc atcactttgg
    31861 catgtccggc atctaacatt cccgccagaa tatcgttgat gatggcacgc ggttcttcgg
    31921 tacgcgggtt atcgtccgtc accaccgcca cgtcagcaaa ctcttcggca attgcgccca
    31981 tcagtggacg cttaccttta tcgcgatcgc caccacagcc aaagacgcac cacagcttgc
    32041 ccgcacagtg caggcgcgcc gcctgtaagg ctttttccag tgcatccggc gtatgcgcgt
    32101 aatccaccac caccgtcggt ttgcctggcg cagtgaacac ttccatacgt ccgcaaaccg
    32161 gttgcagacg cgcggcggtt ttcagcagat cagccagtgg atagccgagt gccagcagtg
    32221 tcgccagcgc gagcagcagg ttgctgacgt taaaagcgcc catcagatgg ctttcaattt
    32281 cgccatcgcc ccaacttgag ctaaagcgaa tcgtcgcacc gctgtcgtga tagttcacgt
    32341 cgatcgcttt taaccagcgt ccgtgacagt tcggattaat atgatcttcc attgataccg
    32401 caaccgcgtc cggcagtttt gccagccagc ggcggcccac ttcatcgtcg gcgttaataa
    32461 tcgcctgacc gcaatgatgt tcagaataaa gcagccattt cgcagcttcg tagtgttcca
    32521 tatcaccatg ataatcaagg tgatcgcggc ttaagttggt aaagaccgac gccgcaaatt
    32581 tcaatgccgc cacacggtgc tgtaccagcc cgtgggagga aacttccatt gcgcaaaacg
    32641 tcgcgccctg ctccaccagc cccgccagct catgctgaac atcgactgcg gaaccggtag
    32701 tattttctgt cgggatcact ttcccaagca gaccgttacc aacggtgccc attaccgcgc
    32761 tggtttcgcc aagcagttgg ctccactgcg ccagcagctg ggtagtcgtg gttttgccgt
    32821 tggtgcccgt tacgcctacg agacgtaaat tgtcagaggg ttcatggtaa aagcggcccg
    32881 ccagtgcaga taaacgctca ttgagctggc tgagatagat gaccggtacg ccgtgcattt
    32941 cacggatttc accgtcggtc gcctcatctt tcgctttcgc ctctgcaata atggcagcca
    33001 caccttgcgc tatcgcctgc gggatatatc gacgcccgtc cgcctgatga cctactacag
    33061 ctacaaagag atcgcccgcc gcagccacac ggctgtcgag tgtcatctct cgcagtgctc
    33121 gcgaaggtgc gtctggcacc cacggagcaa gaaggtcgcg caaattacga tctgccacct
    33181 gtcccctcgc cttgattaat cacaaattca tttttatcgc ccgttgtcag cgcatccggc
    33241 tcgatgttca tggtacgcaa tacgccgccc atgatggcac caaagaccgg cgcggaaacg
    33301 gcgccgccgt agtatttacc cgcctgcgga tcgttgataa caacaaccag cgcgaagcgc
    33361 ggctgactcg caggcgcaac gcctgcggta taagcaatat atttattgat gtagcgaccg
    33421 tccggcccga cctttttcgc ggtaccggtt ttaatggcga tacgatagcc tttaatcgcc
    33481 gccttcacgc cgccgccgcc tggtagcgcc acgctttcca tcatatgcac cacagtgcgg
    33541 acaatggatc ccgggaagac acgttcaccg ggaaccgggg ggtcaacttt ggtaatcgac
    33601 agtgggcgat aaatgccgta gctgccgata gttgcgtaga ctcgcgctaa ctgtaacggt
    33661 gttaccatta gcccgtagcc gaaagagaag gtggccctct ctatgtcaga ccaccgttgt
    33721 ttttgaggat ataagccact gcgttctccg accaacccca aattggtcgc ttttcccagt
    33781 ccaaaacgtg agtaagtatc tactaacgct gaggacggca tcgctaacgc cagcttggaa
    33841 acaccgacgt tactcgactt ctgtaatacc ccggtcaggg ttaattcgct gtagcgtgcc
    33901 acgtctttga tttcgtggcc gttaattcga taaggaatgg tattgagtac cgagttttcc
    33961 cgcaccacgc cacgttgcaa cgcggtcatt accaccatcg gtttaaccgt tgagcccggt
    34021 tcaaacacgt cggtgatggt acggttacgc atcgcctctt tcggcgtgcc gctcagattg
    34081 ttagggttgt atgacgggct gttagccatc gccagcactt caccggtgtt gacatccacc
    34141 agcacggcgc taccagattc agccttgtta aaggccaccg cgttgttcag ttcgcgataa
    34201 accagcgcct gcaggcgttc atcaatactc agcgccaggt tgtgcgctgc ctggctgtca
    34261 gtagaagaaa tatcttcaat tacgcgacca tagcggtctt tacgcacaat gcgctcaccc
    34321 ggctgcccgg taagccattt atcgaaactc ttctcaacgc cctcaatccc ttgactatcg
    34381 acgttagtaa agccgatgag gtgagcagtc acttcgccgg acggatagta acggcgagac
    34441 tcttcacgca gatgaatccc cggcagtttc agttttttga tgtagtccgc catgtcaggg
    34501 ttcacctgac gcgccagata aataaaacgc cctttcgggt tggcgttaat gcgggctgaa
    34561 agctgatcca gcggaatatt gagcgcgtta gccagcgcct tccagcggtc accgacgctg
    34621 ataccgccag cgtcatgcac ttctttcggg tcagcccaaa tcgcttttac cggcacgctc
    34681 accgctaacg ggcgaccaga acggtcagta atcatgccgc gggaggtgga aacttgctga
    34741 acgcgaagag aacgcatgtc gccctctttc accagcatat ccggggagat aacttgtaac
    34801 cacgctacgc gtccgagcag aaaagccagc gccaggagaa tacagccgca taacaacgca
    34861 aaacgccaac tgataaagtt ggcatgttct tcctgacgtt ttggtttctg cgttttcgcc
    34921 gctgctttca tgcgtcgcgt ttatccttat ttttgcacta cgatattttc ttgtgacgga
    34981 tcaacatgct gcatttgcag cttttccgtg gcgatccttt ccacccggct atggtcgccg
    35041 agcgcattct cttcaaggat caggttgcgc cattcaatgt ctaaagcatc tcgctccagc
    35101 accagttgtt cgcgctgagc ggtcagtaaa cgggtatggt gcgccgtggt taccacagtc
    35161 accgccgtca aaataatgca aatgaacagg cagagtggca gcttcccaaa tcgcaaaaga
    35221 tcgtcaccga taacaccagg caatgcatgg cgctcgtggc ttcccatcga tcctttaact
    35281 ttgcttagag cttctgtcac tctgctgatc atgcattcgt cctctctgca atacgcagaa
    35341 ctgaactacg ggcacgaggg ttctcagcca cctcttcttc gcccggcatt aacttgccta
    35401 gtgctcgcag ctgacggcca cccagttttt tgagctgctc ttcagtcatc ggtaaccctg
    35461 ccggaacttg cggaccgcgg ctgttttcac gcataaaacg tttcacaata cggtcttcca
    35521 gcgagtggaa gctgatgatc gaaagccgcc cacccggggc cagcacgttg agcgagcttt
    35581 ttagcgcctg ctctatctcc tccagttcac tgtttaccca aatgcgcacc gcctggaagg
    35641 tacgggtcgc gggatgttta aacttatctt tcaccggcgt tgcaacagcc acgacttccg
    35701 ccagttcttt ggtgcgggtc atcggctgtt cgcggttacg ctcgacaatg gcgcgggcaa
    35761 tgcgtttggc aaaacgctct tcaccatagg ttttcaatac ccaagcgata tcggcttctt
    35821 ctgcggtttg tagccattca gcggctgact gcccacgggt tgggtccata cgcatgtcca
    35881 gcggaccatc gcgcataaag gaaaagccgc gctcagcatc atcaagttgc ggtgaagaga
    35941 cgccaagatc gaggagaatg ccgtcgatct tgccgataag atcgcgctcg gcaacgtatt
    36001 cgcccagcgc ggagaaaggt ccgtggatga tggagaagcg cggatcatca atagtcttcg
    36061 caacggcgat agcctgcggg tcgcgatcga tcgccagcaa acgcccctct tcgccaagct
    36121 gcgagaggat cagacgtgag tgaccaccgc gaccaaaagt cccatcaatg tagatgccat
    36181 caggacggat attgaggcca ttaacggctt catccagcag caccgtagta tgtttatagt
    36241 tttccatcat tttatagaga caagtcctgc agtcgctccg ataagtctcc ggtagccaac
    36301 tgctctgcgt cgatatcttc cttgacctgt tgatgccagg ttgtttcatc ccacagctca
    36361 aacttgttga actgtccaac cagcatcact tcttttgtca gcccggcatg ttgccgcagt
    36421 actggcgcga ttaacaatcg acctgcgcca tccatctgac attcgctggc atgacctaac
    36481 agtaggcgct gcacacggcg ctcaaccggg ttcatgctcg acagacgcga taatttttgc
    36541 tcgataattt cccattcagg cagggggtaa agcagcaggc acgggtgatg aatgtcaatg
    36601 gtgcaaacca tttgaccgac agcgttctca agcagctgtt cccggtaacg ggtaggcact
    36661 gataagcgcc ctttgctgtc gagattgact aacgttgctc cccggaacat gccagcctca
    36721 ccccttattc ccactttgcc ccacaatttc ccactgaaag gagtttacgg agcggaggaa
    36781 aagcttgtca agccagtcac aaggcatacc gaggtaaaaa acccgatgtt tacggctaat
    36841 atcagcgttg tttattttac gtccaacgtg cgcagcaaat taacgttatg aatagctgta
    36901 agaaaaaatc cctcatcgtc atcaccgttt taaaatgact cactattttc cacagcaaat
    36961 ataaagtgtc agtttgcgac gcgagcggca ttttaggaca tatcttcccc ggttaacagt
    37021 ccctgttgcg tctgtgtggc gcttgatgcc agcgggtttg cgcaaaagca aagaaaagtg
    37081 cttgttaaat cggcaattca tctctgcatg taacaaaata atgcaaaaac gcgctgtcga
    37141 tatcacttaa ttattgtgaa tttcagctgg tgagttaatt aacgggcggg tttaaaagaa
    37201 cttatcaata atagtcagga atagtcttat ttactttaag gatattgatg tccattcccg
    37261 tactctacg
//
LOCUS       DAJTZL010000021        36716 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.021, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000021 DAJTZL010000000
VERSION     DAJTZL010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36716
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.021"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..1092
                     /gene="dacC"
                     /locus_tag="OO544_000914"
                     /pseudo
     CDS             1..1092
                     /gene="dacC"
                     /locus_tag="OO544_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
     gene            complement(1139..1897)
                     /gene="deoR"
                     /locus_tag="OO544_000915"
     CDS             complement(1139..1897)
                     /gene="deoR"
                     /locus_tag="OO544_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HCR6766581.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFNPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(1955..2551)
                     /gene="ybjG"
                     /locus_tag="OO544_000916"
     CDS             complement(1955..2551)
                     /gene="ybjG"
                     /locus_tag="OO544_000916"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCR6766582.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRVCFALPIRKGWVRD"
     gene            2836..4063
                     /gene="mdfA"
                     /locus_tag="OO544_000917"
                     /pseudo
     CDS             2836..4063
                     /gene="mdfA"
                     /locus_tag="OO544_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
     gene            complement(4104..4388)
                     /locus_tag="OO544_000918"
     CDS             complement(4104..4388)
                     /locus_tag="OO544_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6766583.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYARHLASVGEGMVIEHQIDDHHH"
     gene            complement(4474..5289)
                     /gene="ybjI"
                     /locus_tag="OO544_000919"
     CDS             complement(4474..5289)
                     /gene="ybjI"
                     /locus_tag="OO544_000919"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HCR6766584.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLLSFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFTTVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            complement(5289..6497)
                     /locus_tag="OO544_000920"
     CDS             complement(5289..6497)
                     /locus_tag="OO544_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6766585.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTRTAMENS"
     gene            6581..7117
                     /gene="rcdA"
                     /locus_tag="OO544_000921"
     CDS             6581..7117
                     /gene="rcdA"
                     /locus_tag="OO544_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HCR6766586.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            complement(7292..8977)
                     /locus_tag="OO544_000922"
     CDS             complement(7292..8977)
                     /locus_tag="OO544_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HCR6766587.1"
                     /translation="MNINVAELLNGNYILLLFVVLTLGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            9247..9624
                     /locus_tag="OO544_000923"
     CDS             9247..9624
                     /locus_tag="OO544_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HCR6766588.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(9654..9911)
                     /gene="grxA"
                     /locus_tag="OO544_000924"
     CDS             complement(9654..9911)
                     /gene="grxA"
                     /locus_tag="OO544_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HCR6766589.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            10071..10358
                     /locus_tag="OO544_000925"
     CDS             10071..10358
                     /locus_tag="OO544_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HCR6766590.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            10342..11064
                     /gene="nfsA"
                     /locus_tag="OO544_000926"
     CDS             10342..11064
                     /gene="nfsA"
                     /locus_tag="OO544_000926"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HCR6766591.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQCEAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRRDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            11125..12027
                     /gene="rimK"
                     /locus_tag="OO544_000927"
     CDS             11125..12027
                     /gene="rimK"
                     /locus_tag="OO544_000927"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HCR6766592.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            12115..12591
                     /locus_tag="OO544_000928"
     CDS             12115..12591
                     /locus_tag="OO544_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HCR6766593.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            12942..14054
                     /gene="potF"
                     /locus_tag="OO544_000929"
     CDS             12942..14054
                     /gene="potF"
                     /locus_tag="OO544_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HCR6766594.1"
                     /translation="MTALNKKWLSGLAAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            14149..15282
                     /gene="potG"
                     /locus_tag="OO544_000930"
     CDS             14149..15282
                     /gene="potG"
                     /locus_tag="OO544_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HCR6766595.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIASRVHEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            15292..16243
                     /gene="potH"
                     /locus_tag="OO544_000931"
                     /pseudo
     CDS             15292..16243
                     /gene="potH"
                     /locus_tag="OO544_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415377.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
     gene            16240..17085
                     /gene="potI"
                     /locus_tag="OO544_000932"
     CDS             16240..17085
                     /gene="potI"
                     /locus_tag="OO544_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HCR6766596.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDGITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMVRAEKQRIRDIQ
                     RARHG"
     gene            17145..17633
                     /locus_tag="OO544_000933"
     CDS             17145..17633
                     /locus_tag="OO544_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HCR6766597.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            17674..18801
                     /gene="rlmC"
                     /locus_tag="OO544_000934"
     CDS             17674..18801
                     /gene="rlmC"
                     /locus_tag="OO544_000934"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HCR6766598.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSETKLAQLRKALPWLQE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIASEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGDVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVMTLLVKQ"
     gene            complement(18976..19707)
                     /gene="artJ"
                     /locus_tag="OO544_000935"
     CDS             complement(18976..19707)
                     /gene="artJ"
                     /locus_tag="OO544_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein ArtJ"
                     /protein_id="HCR6766599.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDSVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(19999..20667)
                     /gene="artM"
                     /locus_tag="OO544_000936"
     CDS             complement(19999..20667)
                     /gene="artM"
                     /locus_tag="OO544_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HCR6766600.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLALLLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(20667..21383)
                     /gene="artQ"
                     /locus_tag="OO544_000937"
     CDS             complement(20667..21383)
                     /gene="artQ"
                     /locus_tag="OO544_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HCR6766601.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(21390..22121)
                     /gene="artJ"
                     /locus_tag="OO544_000938"
     CDS             complement(21390..22121)
                     /gene="artJ"
                     /locus_tag="OO544_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6766602.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDGVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(22139..22867)
                     /gene="artP"
                     /locus_tag="OO544_000939"
     CDS             complement(22139..22867)
                     /gene="artP"
                     /locus_tag="OO544_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HCR6766603.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(23085..23600)
                     /locus_tag="OO544_000940"
     CDS             complement(23085..23600)
                     /locus_tag="OO544_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR6766604.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATLLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            24249..26018
                     /locus_tag="OO544_000941"
     CDS             24249..26018
                     /locus_tag="OO544_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCR6766605.1"
                     /translation="MINNISDQASSFPGTQLNQSDNFLDSLREFFAILNSSRKGELST
                     WDTIYLHLILAINADSDLIKNDVLLAENMPSANYQFNTFFSNAFETDVKKYLGKSEDN
                     EVEIKAGNERISIGIRNVSNGKLERQQFLFPLDYENKLQDQLDKHFTIESHPLLYRHT
                     IGSKIANVIFEKLYSRIDFNKEQYISFIKGAFIHFYDYSRRYAISENIEKDAVTNNIA
                     LMNTFYDSDNTSGEVLNNDFTEEEPFETALDVEHTIVLGFADDNFETKPVHYQDLLTR
                     FSAFQDTVFNLFPEMRSSHYHDICSVCVDMTKGTQCMIHLMVNEEVFMSLPVPVATMV
                     REDASNLVNLKTLLNDGFFIKYSHFNDVALIKQNISNLYLSHTVVNESILKKCCFENG
                     SLGDVKITNSNIINNVFKNVSFRSVKINNVNTHSLKFINCHFFNVDMIRVNFSKCLFH
                     DCSMHGVKIKPWLPVKWTKELINDYLYGCLLSLYSICARDIYNMSAGNNVKVAADAFL
                     EIIFSLKNKYCIKLLSAQERAFIYEFARMIFAYINDKSIEILLLSCFAASDQKAIQRY
                     IPQSQDGEDFRSHLQYKLPLSAH"
     gene            26229..26552
                     /locus_tag="OO544_000942"
     CDS             26229..26552
                     /locus_tag="OO544_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HCR6766606.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            26549..27379
                     /gene="amiD"
                     /locus_tag="OO544_000943"
     CDS             26549..27379
                     /gene="amiD"
                     /locus_tag="OO544_000943"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HCR6766607.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPTQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(27376..28389)
                     /locus_tag="OO544_000944"
     CDS             complement(27376..28389)
                     /locus_tag="OO544_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCR6766608.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENTVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPIITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(28488..29918)
                     /locus_tag="OO544_000945"
     CDS             complement(28488..29918)
                     /locus_tag="OO544_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HCR6766609.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPASEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWQVVNQIGGKERYFFGNILWQTRALMDRAIGHKLAKGRPEREYLQTGDAVD
                     SWKVIVVEPEKQLTLLFGMKASGLGRLCFSLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            complement(29929..30930)
                     /gene="ltaE"
                     /locus_tag="OO544_000946"
     CDS             complement(29929..30930)
                     /gene="ltaE"
                     /locus_tag="OO544_000946"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HCR6766610.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRKRN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(30967..32685)
                     /gene="poxB"
                     /locus_tag="OO544_000947"
     CDS             complement(30967..32685)
                     /gene="poxB"
                     /locus_tag="OO544_000947"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HCR6766611.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKADRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIVVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(32818..33786)
                     /gene="hcr"
                     /locus_tag="OO544_000948"
     CDS             complement(32818..33786)
                     /gene="hcr"
                     /locus_tag="OO544_000948"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HCR6766612.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(33798..35450)
                     /gene="hcp"
                     /locus_tag="OO544_000949"
                     /gene_synonym="priS"
     CDS             complement(33798..35450)
                     /gene="hcp"
                     /locus_tag="OO544_000949"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HCR6766613.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKACEYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGDDFSAVITQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            complement(35594..36493)
                     /gene="lysO"
                     /locus_tag="OO544_000950"
     CDS             complement(35594..36493)
                     /gene="lysO"
                     /locus_tag="OO544_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HCR6766614.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
BASE COUNT         9144 a         9061 c         9473 g         9038 t
ORIGIN      
        1 atgcgcgtgc atggatttta atggattacg ccagcggtaa agtgctggca gaaggcaacg
       61 cggatgagaa actggatccc gcgagcctga ctaaaatcat gaccagctat gtggttgggc
      121 aggcgcttaa ggccgataag attaaactca ccgatatggt gacggtcggt aaagatgcct
      181 gggcgacggg aaatccggca ctgcgtggtt catcggtaat gttcctcaaa ccgggcgatc
      241 aggtttcggt ggcagacttg aacaaaggtg tgattatcca gtccggtaat gacgcctgta
      301 ttgcgctggc cgattacgtt gccgggagcc aggagtcatt tattggtttg atgaatggtt
      361 atgccaaaaa aactgggtct gaccaacact accttccaga cggtgcacgg tctggatgcg
      421 ccggggcagt tcagtaccgc gcgcgatatg gcattgctgg gtaaagcatt gatccacgat
      481 gtgccggaag agtacgccat tcataaagag aaagagttca ccttcaacaa aattcgtcag
      541 cctaaccgta accgtctgct gtggagcagc aatctgaatg ttgatggcat gaagacagga
      601 accacggcag gtgcgggata taatctggtt gcttcggcta cccagggtga tatgcgttta
      661 atctccgtag tgctgggggc gaaaactgac cgtatccgtt ttaatgagtc tgagaaatta
      721 ttgacctggg gtttccgctt ctttgaaact gtgacgccaa ttaaacctga tgccaccttt
      781 gtgactcagc gcgtctggtt tggtgataag agcgaagtga atctcggggc aggcgaagcg
      841 ggctccgtga ccataccgcg tgggcagctg aaaaacctga aggcgagcta cacgttaacg
      901 gaaccgcagc ttaccgcacc gctgaaaaaa ggtcaggttg tcgggaccat tgatttccag
      961 cttaacggta aatccattga gcagcgtccg ctgatcgtga tggaaaatgt ggaagagggc
     1021 ggattctttg gtcggatgtg ggatttcgtg atgatgaaat tccatcagtg gttcggcagc
     1081 tggttctctt aatcttctga taaccggatg gcgcgaaacg tcatccggtt atacgtcatt
     1141 aatacatcaa cttaatacgg tgcgtctgcg cgtacttcac atactcatct tccgggcaac
     1201 aatcactcac cacaatatca aagcgtttca ggtcacccat gcgcgccgga cgcaccttgc
     1261 caaatttact gtggtcgaca accagcacat gcttttgcgc catcgacatg gcccagtgtt
     1321 ttaccggcaa ctcttcaaga ttaaaacagg tagcgccttt actgacatgc acgcccgccg
     1381 cggaataaaa agcgatatcc gggcaaaaat tattcaacgt ttgctgaaaa tcgatggggt
     1441 tgaaaatggc gttgctggcg tgaaattcac caccgcacag aaatgcgcgg caatggggtt
     1501 tctctttcag cgccagaaag gtatttagcg aataacaaac ggcggtaaaa gggatttcat
     1561 tatcaatcgc ttcaataatc cacggcgtgg tggtgccaca gtcaaaaaag agggtctgat
     1621 cgggttctac cagtgtcgca gccagttttg ccgcccggcg tttttcttcc accaggcggg
     1681 atttttgatc gcttaacagg taatggctgg cactgcgcgg ttccagaaca atatagccgc
     1741 cgagcaaaac gacgggcgca ctgtggttgt tcagatcgcg acgaatcgtc atctccgaaa
     1801 ccccaagcag ggcggcggcg tctttaagat gtaacttatc gctgcgtttt aattcttgca
     1861 gcagctgccc gatacgctct tcgcgacgtg tttccataat ccctctgaat agttattgaa
     1921 gcgagccgct caatactaca ctttttagca gagatcagtc acgcacccag cctttgcgga
     1981 tcggtaatgc aaaacagacg cgataccacg attgcagacg ttgatagagt ttatgcccca
     2041 tcgcttgcca gataatctgg gcactaaggc agccaatcat acctgccagc aatccaccga
     2101 gcatatccag cggccagtgg acgccaagat aaacgcgcga ccaggcaatg acgacggcca
     2161 gaaccattaa aagtgagccg gaccacaggc gatgccagca taaaaatgcc agtgcaaagg
     2221 tgaaaatcac cgtaccgtga tcgctcggga atgagtcatc cgccgcatga tgcaggaagt
     2281 tatagccgat attttcgaca aagggtcggt cgtgcggaaa aagatgtccc atcgtccagg
     2341 acacaaacag gctgaccgcc agcgcgatag cgatttttat caccagttgc cgttgtgctg
     2401 taagccccca caaccaaagt accgcggcca gcaacggcac cacggtaatc aaatctttag
     2461 caataaaaat cgccaacgag atcatccacg gagccgagtc tggcgtcgcg ttaataagag
     2521 agaatagaga gagattcaaa ttttccagca taacttcccg acgcaaagtg attaaaaggg
     2581 agccaataca ggcaagtcgt tgagaataaa gtgcaggtta aactgggtaa agcggcatcg
     2641 tcttatttcc ctcaagcggc ctgtttacgg tgggtgattg taacgggcat aggttaaata
     2701 aaacttaaag aaagcgtagc tatactcgca ataatgtaag aatgtgctta accgtggttt
     2761 cagctacaaa attcgctttc tcgttagctg cgcttttatt aaactctgcg cgattattat
     2821 tggcgaagaa attgcatgca aaataaatta gctttcggtg ccaggcttgg acgtcaggcg
     2881 ttacttttcc ctctctgtct ggtgctttac gaattttcaa cctatatcgg caacgatatg
     2941 atccaacccg gtatgttggc cgtggtggaa caatatcagg cgggcattga ttgggttcct
     3001 acttcgatga ccgcgtatct ggcgggcggg atgtttttac aatggctcct ggggccgctg
     3061 tcggatcgta ttggtcgccg tccggtgatg ctggcgggtg tggtgtggtt tatcgtcacc
     3121 tgtctggcaa tattactggc gcaaaacatt gaacaattca ccctgttgcg cttcttgcag
     3181 ggcataagcc tctgtttcat tggcgctgtg ggatacgccg caattcagga atccttcgaa
     3241 gaggcggttt gtatcaagat caccgcgctg atggcgaacg tggcgctgat tgctccgcta
     3301 cttggtccgc tggtgggcgc ggcgtggatc catgtgctgc cctgggaggg gatgtttgtt
     3361 ttgtttgccg cattggcagc gatctccttt ttcggtctgc aacgagctat gcctgaaacc
     3421 gccacgcgta taggcgagaa actgtcactg aaagaactcg gtcgtgacta taagctggtg
     3481 ctgaagaacg gccgctttgt ggcgggggcg ctggcgctgg gattcgttag cctgccattg
     3541 ctggcgtgga tcgcccagtc gccgattatc atcattaccg gggagcagtt gagcagctat
     3601 gaatatggct tgctgcaagt gcctattttc ggggcgttaa ttgcgggtaa cttgctgtta
     3661 gcgcgtctga cctcgcgccg caccgtacgt gataattatg ggcggctggc cgattatgat
     3721 tggtctgttg gtcgctgctg cggcaacggt tatctcatcg cacgcgtatt tatggatgac
     3781 tgccgggtta agtatttatg ctttcggtat tggtctggcg aatgcgggac tggtgcgatt
     3841 aaccctgttt gccagcgata tgagtaaagg tacggtttct gcggcgatgg gaatgctgca
     3901 aatgctgatc tttaccgttg gtattgaaat cagcaaacat gcctggctga acgggggcaa
     3961 cggacagttt aatctcttca accttgtcaa cggaattttg tggctgtcgc tgatggttat
     4021 ctttttaaaa gataaacaga tgggaaattc tcacgaaggg taaaaaaatg cctgactgct
     4081 ttgtgcgatc aggcattctc gaattaatgg tgatggtcgt caatctggtg ttcgataacc
     4141 atcccttcac ctacgctggc aagatggcga gcataaggat gcgggcggta agccggagct
     4201 ggcgcaggag ccacatagac ggtttgcggc acagtcgcga cgctgaccgc ttgtggaacg
     4261 ctgactgaat caggaactac aaccactttg tatccactcg gcacatcgac ggtgacactt
     4321 tgcgccatcg ccacaccagt aaagcccatt aaaagcgcgc ccaacagtag acaatttttc
     4381 ataatcattt cgcctgaaat aatgcagcgg gttaatttcc gcgaattatg cagccttcat
     4441 tcaggtattg tgttgccggg cgtgctgccg tttttattgg tcaaatggcg cttcgtgttt
     4501 aagcacttta tcgatcacat ccagtacgcc ttcacggtta ttggagcctg cccggtattt
     4561 tgccgctgcg acgaccgcgc tgccggcatt ttccattgca aaactaaagc ctgcctgacg
     4621 cagcatctca atatcgttac cgccatcgcc aaagaccacc acttcgctgt cgtctattcc
     4681 ccataatttc tgcagttggc gaaggccatt ggctttatgt acgccgggga taatcagatc
     4741 gatgctgccg ttgccggtgt ggactggcac cataatatcg ccgatggcct catgtaatgc
     4801 tttttgtact tgtggaatca gttcatcgga aagattcagg ccaaacttaa agaaaatatc
     4861 ttctaagttg tcaaagttat cgacgtattc cagacggtga taatacattt ctgccaccgt
     4921 tttcatggca tcgtcatatt ttttgagcgt ataggcgcta ttttttccgc aggcaataat
     4981 ttccacttcc gggcgcgtca gcaaatgttc cacgacagta gtaaaggcat ccttcgacag
     5041 ctcgccatta aaaacatctt tgccttcact cactacccag ccgccgtttt cagccacaaa
     5101 ggcaatttca ttagcaattt cagggaagaa agagagcaac tgataatatt gattcccgct
     5161 ggcgaccaca aagcgaatgc cttgtgcttt catttgctga tactgagcca taaaccgctc
     5221 acggttatag gttttttgat cgcttaagaa agtaccatcc atgtctaccg caattaattt
     5281 aatgctcatc aactattctc catcgccgtc cgcgttttgg tatcgggttt ggcgacggct
     5341 ttcgcgacaa tagccgcgag aataaccagc gccagtacaa ccagcattgc actacgtaat
     5401 ccataatgtt cgccgagata gcccagcagc ggcggcccga cgaggaaagc cagataaccg
     5461 gtcgttgcta ccacactgac gcgggttggt gcatcggggc cggtatcgct ggcggcagaa
     5521 atggtcagcg ggaagcccag cgaggcaccc agtccccaga gtacaacaga caccccagcg
     5581 acccaggcgc tatcgacaaa aataatcagc ccaataccca acgcccccat tagtgcactg
     5641 gcccgaacca cggcaacgcg actgtaacgg tcgatgaacc aaccgccagt gaagcgtcca
     5701 acggtcatcc ctagggtaaa accggcataa atcagcgagc cggaagtagg gctaaaaccg
     5761 tgaccgtcaa ccattaataa gggtaaccag tcgttggcag aaccttcggc aaaggccatc
     5821 gccagcacta caacaccaat caacagcaac tggatatcgc gataaaaagg aatgcctttt
     5881 tcgccatgct gggtgccatc ggcagcattt ttgcccgtac cgtcagggat tgcctgaatg
     5941 gcgatataaa taggcgcgat acctaccagc gccgccaata aaatgtgcac cgttgccgga
     6001 acgccaaagg ccgtcagtgc catcccgaca ccagcgcctg ccagcgtgcc taggctataa
     6061 aaaccgtgca tcatcggcaa aaccgtttta ttcatttctc gctcaacggc ggcaccttca
     6121 acgtttatcg ccacttccgc agaaccaaaa cttgccccaa agacgccgag accaacggca
     6181 aagagcaggg gcgatgtcag ccagagtgcc agacttaata tcatcatccc gatcaatgcg
     6241 caggacatcg tgaccaggat gacattacgt gtcccaaagc gtttcactaa ccacgccgag
     6301 cagagaatac cgctcatcga accgatcgac agaccaaaga gaacaccgcc catttcagcg
     6361 atcgagacag agagaatatc gcggatagca ggcgtacggg ttgcccagga cgccattaac
     6421 aggcctggca aaaagaagaa cataaacagc gcccaggttc ggcgtttcaa tgcattacgt
     6481 gaagaattta cggtcataga tcacgtcaaa ataagaagag ggaagacaac attagcaagg
     6541 ttgtgtacat ttgtacacaa ttgcagacag aggaaatgac atgcgtcgcg ctaacgatcc
     6601 gcaacggcga gaaaaaatta tccaggccac gctggaggcg gtgaaacttt acggaataca
     6661 tgctgttacg caccgcaaaa ttgctaccct tgccggggta ccgttgggat cgatgaccta
     6721 ctatttttca ggaattgatg agttgttgct ggaggcgttc agcagtttta ctgagattat
     6781 gtcccggcaa tatcaggcat tttttagcga tgttagtgat gctcaaggcg catgccaggc
     6841 tatcaccgat atgatctaca gctcacaggt tgcaacgccg gataatatgg agctgatgta
     6901 ccagctctac gcgctggcta gccgaaaacc gctattaaaa acggtaatgc aaaactggat
     6961 gcagcgcagt cagcaaacgc tcgaacaatg gtttgaaccc ggaaccgccc gcgcgcttga
     7021 tgcgtttatt gaggggatga cgctgcattt tgtcaccgac cgtaagccgc tatcgcgcga
     7081 ggagattttg aggatggttg agagggttgc agggtagtag ataaatttta gataacaaaa
     7141 aacccatcaa ccttgaaccg aaatggcggg gttgatgggc tccacaaaat ggggacatca
     7201 aagaaaagca gtggcactaa ttaagactga tgccctgcgg aaaagttctg cggttgtgca
     7261 aaaaaatttc attttcaggg caacttcagt tttatcctaa tcctggccat accatgacga
     7321 tgattgtccc tgccagcgtc agcaggacgt tggcgattgc gtaggtgccc gcatagccca
     7381 gcgcagggat gttactgcga gctgtatcac tgatgatctc cattgccggc gcgcaggtgc
     7441 gtgcgcccat cattgcgccg aacaacagtg cgcggttcat tcgcaataca taagcaccga
     7501 acaagaaaca gataaccacg ggcacaagac tgacaattaa tccggcaatc aacatctgac
     7561 cgccaatcgc gcccaggccg ttattaatac cgctaccggc gctcagacca acgcctgcca
     7621 taaacaccat caagccgaac tctttcacca tgcttaatgc accttgcgga atgtaaccga
     7681 aggtcgggtg gttagcacgc ataaagccca gcataattcc ggcgaataac aacccggcag
     7741 cgttccccat gccgaaactg aatgtgctga actggaaggt gatcatcccg atcatcagcc
     7801 caataacaaa gaaggcgcaa aatgccagca ggtcagtgac ctggctgtga atcgagataa
     7861 agccgatgcg atcggcgatg gtttttacgc gacgggcatc accgctgact tgtaaaacgt
     7921 cacctttgtt aagcacgacg ttgtcatcta tcggcatctc aatctggcta cgaatgacgc
     7981 ggttaaggaa gcaaccgtga tcggtcaact tcagttgtgc gagacgttta cctacagcgt
     8041 tatggttttt aacgaccact tcttcagtga cgatacgcat gtcgagaagg tcacgatcga
     8101 aaacttcttt accgttacgg aagctgggat cgagtcgggc atgggcgtcg ggatagccta
     8161 ccaacgctat ttcatcgccc atttgtagca cggcatcacc gtctggattt gccagaatcc
     8221 cgttacgtcg aatacgttca atgtagcagc cggtttgtcg ataaataccc agttcacgca
     8281 gatttttgcc gtcggtccag gccaccagct ccgggccgac gcgataggcg cggatcaccg
     8341 gtaaataaac cttacggttg gcatcagtgt ccaggccacg ttcgcgggcg atttgctggg
     8401 cgctggtctg taagtcctga tgctgcaatt tcggcaagta acgcgcacca acaatcaaac
     8461 tcaccagacc gattaaatag gttaaggcat acccgaggct cagattatcc agtgccagtg
     8521 agagctgcct gctttccatg ccggaatgac gcagtgtatc gccagcaccg accagaaccg
     8581 gtgtcgacgt catagagcct gctaacatac cggccgtcag gccaatatcc cagccaaaca
     8641 gcttacctaa ccctaaggcg atcaccagcg cactgccaac catcaccagt gctaacatta
     8701 ggtaattttt cccatcgcga aaaaaaatgg aaaaaaagtt cggtccggct tcgaccccga
     8761 cgcagaaaat aaacagcata aagccaagat taagcgcatc ggtgttaatg ctgaaatgtt
     8821 gttggcctaa taacagcgat acgactaaaa cgccaatgga attacccagt tggatcgaac
     8881 caagtcgtaa ctttccgaga catagcccaa gcgtgaggac cacaaataat aacagaatgt
     8941 aattcccatt taacaattcg gcgacgttta tattcacgga ggctaacttc ttgtttacta
     9001 gtaagctgtt gaaagaaatg gtaatttacg ataatgtttt ttaccagaat tcagggcgca
     9061 gattcattca gcgcacctaa acgatagtaa agtaacaata tattttacta gtgtaatcac
     9121 attaggtatc aacggctata tgaattgcgt tggcctatat tagcatggaa tgcgaagcgg
     9181 ctttatctta ctgaacgcca cactggcgaa aaatgtgttc gatagacgca gtgtcaggag
     9241 gaacgagtga aacataaaca acgttgggcg ggggcaatct gctgttttgt cctcttcatt
     9301 gtggtgtgcc tttttctggc gacgcacatg aaaggcgctt ttcgggctgc cgggcatcct
     9361 gaaatcggct tgctgttttt cattcttcct ggagcagttg ccagtttctt ttcacagcgt
     9421 agagaagtcc tgaaacctct atttggcgca atgctggcgg caccctgttc aatgctcatt
     9481 atgcggctgt ttttttcacc gacgcgctca ttctggcaag agctggcatg gttactaagc
     9541 gcggtgttct ggtgtgcgct gggggcactg tgtttcttat ttatcagtag tttgtttaaa
     9601 ccacagcaca gaaaaaatca gtaaagccct caacgcgagg gcttgtcaga cgatcaggcg
     9661 tccagatttt ctttcaccca tgcagcaaaa tcggtatagc cgccgatatg ttgctgatcg
     9721 acaaaaatct gcggcacggt ttctacgggt ttacctgcct tttgttgtag atcttcttta
     9781 gtgatccctt ccgcacgaat atctacatac tgatactgaa aatcatcgcg ttcattgctc
     9841 aatttctcag ccagatcttt tgcacgcaca cagtaagggc aacccgaacg accaaaaata
     9901 acggtttgca ttatttctct cctcatagat ttatgcctgt aatgatcacg ctaaaatgta
     9961 ttcgctgaaa gtaggtttaa cctgttgcat taattgctaa aagctataac tgttaaacac
    10021 aatacagtga aaagttttag actgaaggct cactttgcag agggaagcgt atgcgcgcga
    10081 tcggtaaatt gcctaaaggc gtgttgatac tggaatttat cggaatgatg ctactggcgg
    10141 tggcgctgct gtcggtaagc gactccctgt cgctgcctga gccattttct cggccagaag
    10201 tgcagattct gatgattttt ctcggtgttt tgctcatgct tcccgctgcg gtggtggtta
    10261 ttcttcaggt ggcaaaacgt cttgccccac agctgatgaa ccgtccaccg caatattcac
    10321 gttcagaaag agaaaaagat aatgacgcca accattgaac ttatttgtgg ccatcgctcc
    10381 attcgccatt tcactgatga acccatttcc gaagcgcagt gtgaggcgat tattaacagc
    10441 gcccgtgcga cgtccagttc cagttttttg cagtgtagta gcattattcg cattaccgac
    10501 aaagcattac gtgaagaact ggtgacgctg accggcgggc aaaaacacgt agcgcaagcg
    10561 gcggagttct gggtgttctg tgccgacttt aaccgccatt tacagatctg tccggatgct
    10621 cagctcggcc tggcggaaca actgttgctc ggtgtcgttg atacggcaat gatggcgcag
    10681 aatgcattaa ccgcagcgga atcgctggga ttgggcgggg tatatatcgg cggcctgcgc
    10741 aataatattg aagcggtgac gaaactgctg aaattaccgc agcatgttct gccgctgttt
    10801 gggctgtgcc ttggctggcc tgcggataat ccggatctca agccgcgttt accggcctcc
    10861 attttggtgc atgaaaacag ctatcaaccg ctggataaag acgcactggc gcagtatgac
    10921 gagcaactgg cggaatatta cctcacccgt ggcagcaata atcgccggga tacctggagc
    10981 gatcatatcc gccgaacaat cattaaagaa agccgcccat ttattctgga ttatttgcac
    11041 aaacagggtt gggcgacgcg ctaaaaccgc cacgtcgatg tatgatacgc gggcttttga
    11101 ccaggtctga cagagaggtg cagggtgaaa attgccatat tgtcccggga tggaacgctc
    11161 tattcgtgta agcggctgcg tgaagccgct atacagcgcg gtcacctggt tgaaattctt
    11221 gatccgcttt cttgctacat gaacataaat cctgcggcgt cttctattca ctacaaaggc
    11281 cgcaagttac cccattttga cgcagtgatc ccgcgtattg gcaccgccat taccttttat
    11341 gggacggcgg cactgcgcca gttcgagatg ctggggagct atccgctcaa tgagtcggtc
    11401 gccattgccc gggcgcgtga caaattgcgt tccatgcaac tgctggcgcg tcagggcatc
    11461 gacctgcctg tcacgggcat tgcgcattcg ccggatgata ccagcgattt aatcgacatg
    11521 gtcggtggtg cgccgctggt ggtcaagttg gttgaaggca cgcagggaat tggcgtcgtg
    11581 ctggcggaga cgcgtcaggc ggcggaaagc gtgattgacg ctttccgcgg tctgaacgcg
    11641 catattctgg tgcaggaata tatcaaagag gcgcaagggt gcgatatccg ctgtctggtt
    11701 gttggcgatg aagtggtcgc tgcgattgaa cggcgggcga aagagggcga ttttcgttcc
    11761 aatttgcatc gtggcggcgc ggcaagcgtc gccagtatca caccacagga gcgtgaaatc
    11821 gcgataaaag ccgcgcgaac gatggcgctg gacgttgctg gtgtggatat tctgcgtgct
    11881 aatcgcgggc cgttggtgat ggaagtgaat gcgtcgccgg ggctggaagg aatagaaaaa
    11941 accactggta tcgacatcgc gggtaaaatg atccgctgga tcgaacgcca cgctacgaca
    12001 gaatattgcc tgaaaacggg tggttagtcg caatcacatt actgatcatg gttttgcctg
    12061 cgctttttgc gtaagctgtg ccggtctttt tatcgaaaga ggttgtacaa aattatgaca
    12121 tcgctggtcg ttcctggtct ggatacgctg cgtcaatggc tcgatgacct ggggatgagt
    12181 ttttttgaat gtgataactg tcaggctctg catctgcccc atatgcagaa tttcgacggt
    12241 gtctttgatg ccaaaatcga tctgatcgat aacacgatcc tgttttctgc catggcggaa
    12301 gtccgacctt cagccgtatt gccgctggcg gcggatttat ctgccatcaa tgccagttcg
    12361 ctgaccgtga aagcatttct tgatatgcag gatgataatc tgccaaagct ggtggtttgc
    12421 cagtctttat ccgttatgca gggcgtaacc tatgagcagt ttgcatggtt cgtgcgtcag
    12481 agcgaagagc agatttcgat ggtcattctt gaagctaatg cccatcaact gctgttaccg
    12541 actgacgatg aagggcaaaa caacgttacc gaaaactatt tcctccactg ataactcctt
    12601 tcgagcacgc agtcgctggt gcagtggctg cgcgctgcaa aattatctgc tgtttttaac
    12661 cttttcttaa agattatttc acttctcttg tgtcgatttg gctttatcac atagagcaaa
    12721 tacgcataaa aatttgttaa ataccgtttt ttaatccgag ctatagtctc aaaccctggc
    12781 taaagttatt cttgcgatgc ttttatatag cgagcagtgc tggccgggag agagttctct
    12841 tttcttacac cccgccgata aaaaatatgc acgtttattg catatctttt agtgtgacaa
    12901 cttttgttcg tttgttaacg aactttcaga aggaaagaga tatgaccgcc ttaaataaaa
    12961 aatggctttc gggtctggct gcgggtgctc tgatggccgt ctctgtcggc acgctcgcgg
    13021 ctgaacaaaa aacactccac atttataact ggtctgatta tatcgccccg gacacggtgg
    13081 ccaattttga aaaagaaacc ggtattaaag tcgtctacga tgttttcgac tctaacgaag
    13141 tactggaagg caaattaatg gcgggtagca ccggctttga cttggtggtt ccatctgcca
    13201 gctttcttga acgccagttg actgcgggag ttttccagcc gctggacaaa agcaaattgc
    13261 cggagtggaa gaatctcgac ccggagctgc tgaagctggt cgccaaacac gatcccgaca
    13321 ataaatttgc tatgccctat atgtgggcga cgaccgggat tggctataac gttgataaag
    13381 ttaaagctgt gctgggcgaa aacgcgcccg tcgatagctg ggacttgatc ctcaaacctg
    13441 aaaatctgga aaaactgaaa agctgcggtg tctctttcct ggatgcgcca gaagaagttt
    13501 ttgctaccgt gttgaattat ctcggcaaag atcccaacag cactaaagcg gatgattaca
    13561 ccggaccggc aaccgatctg ctgttaaagc tgcgcccgaa cattcgttat ttccattcat
    13621 ctcaatacat taacgacctg gcaaacggcg atatttgcgt cgctatcggc tgggcaggtg
    13681 atgtctggca ggcgtcaaac cgcgcgaagg aagcgaagaa tggagtgaat gtctcgttct
    13741 cgattccaaa agaaggggcg atggcgttct ttgatgtatt cgccatgcct gcggatgcca
    13801 aaaacaaaga cgaagcctat cagttcctga attacctgct gcgcccggat gtagtagcgc
    13861 atatttccga ccatgtgttc tatgccaacg ccaataaagc agccacgccg ctggtgagtg
    13921 cggaagtccg tgagaacccg ggtatttatc ctccggcgga cgttcgtgcg aagctattca
    13981 ctctgaaagt acaggatccg aaaatcgacc gtgtgcgcac ccgcgcgtgg accaaagtga
    14041 agagcggaaa ataatccgca gccttagatg ccgggcgggc gcaccacacc agccggcaat
    14101 tcgcaccatc atggtgcgct tgcacacatt caatgccgga gagcagccgt gaatgacgct
    14161 atccctcgcc cgcaggcgaa aacccgtaag gcgctgacgc cgctattaga aatccgcaac
    14221 ctcaccaaat cctacgatgg tcagcatgcg gtcgatgatg tcagcctgac catctacaaa
    14281 ggtgaaatct tcgcgctgct gggcgcatcc ggctgtggca agtccacgct gctgcgtatg
    14341 ctggcaggtt ttgaacaacc ttctgccggg cagataatgc tcgatggcgt cgatttgtca
    14401 caggtgccgc cttacctgcg ccccatcaat atgatgtttc agtcttacgc gctgtttccg
    14461 catatgaccg tggaacagaa catcgctttt ggcctgaaac aggacaaact accgaaagcg
    14521 gaaattgcca gtcgggtcca tgagatgctc ggcctggtgc acatgcagga gttcgccaaa
    14581 cgcaaaccgc atcagctttc cggtggtcag cgacagcgtg tggccctggc ccgaagcctt
    14641 gccaaacgcc cgaaactatt actgctcgat gagccgatgg gcgcgctgga taaaaagctg
    14701 cgtgaccgga tgcagcttga agtggtggat attctggagc gcgtcggtgt gacttgtgtg
    14761 atggtcaccc acgatcagga agaggcgatg accatggcgg ggcgtatcgc cattatgaat
    14821 cgcgggaaat ttgtccagat tggcgagccg gaagagatct acgagcatcc aactacccgc
    14881 tacagcgctg aatttatcgg ttcggttaat gtctttgaag gtgtactcaa agagcgtcag
    14941 gaagatggcc tggtgcttga ttcgccgggg ctggtgcatc cactgaaagt cgatgcggat
    15001 gcttcagtgg tcgataacgt gccggtacac gtagcgctgc gcccggaaaa aatcatgctt
    15061 tgcgaagagc cgcccgccaa tggttgtaac ttcgcggtgg gggaggtgat acacattgcc
    15121 tatctcggcg atctttcggt gtatcacgtt cgactgaaaa gtgggcagat gatcagcgcc
    15181 cagctacaaa acgcccatcg tcatcgtaaa gggttaccga cctggggcga cgaagtgcgt
    15241 ttgtgctggg aagtggacag ctgtgtggtg ctgacggttt aaggagcaaa gatgagtaca
    15301 cttgaacctg ctgcccagtc gaaaccgccg ggcggattta agctgtggtt gtcgcagctg
    15361 caaatgaagc atgggcgcaa actggtcatt gcgttgctat atatctggtt gatcttgctg
    15421 tttctgctgc catttctgat tgtctttaaa ataagcctgg gagagatggc gcgctattcc
    15481 accttatacc gaactgatgg agtgggctga cgggcaactt tccatcactc ttaatctcgg
    15541 taattttctg caactgaccg acgatccgct ctattttgat gcttatctcc agtcgttaca
    15601 ggtggcggcg atttcgacat tttgctgttt actgatcggc tatccgctgg cgtgggcggt
    15661 ggcgcacagt aagccttcga cccgtaatat tttattacta ctggtgatcc tgccgtcgtg
    15721 gacctcgttt ctgatccgcg tttatgcctg gatgggaata ttaaaaaaca acggtgtgct
    15781 gaataatttt ctgctgtggc tgggggttat cgatcaaccg ctgaccattc tgcataccaa
    15841 tctggccgtt tatatcggca ttgtttacgc ttacgtgccg tttatggtac tgccgattta
    15901 taccgcgttg attcgtattg attattcgct ggtggaagca gcgctggatc tcggtgcacg
    15961 accgctgaaa acgttcttta ctgtgatcgt gccgctgact aaaggtggga ttattgccgg
    16021 atcgatgctg gtgtttatcc cggctgtggg cgagtttgtg atcccggaac tgctcggtgg
    16081 cccggacagc atcatgatcg ggcgcgtgtt atggcaagag tttttcaata accgcgactg
    16141 gccggtggcc tcggcggtag cgatcatcat gttgctgctg ctaattgtgc cgataatgtg
    16201 gttccacaaa caccagcaaa aaagcgtggg agaacacgga tgaataattt accggtagtt
    16261 cgttcgccct ggcggattgt gattttgctg ctgggcttta cctttctcta cgcgccaatg
    16321 ctgatgctgg ttatctattc gtttaacagc tcgaagctgg tgacggtgtg ggccggctgg
    16381 tcaacgcgct ggtatggtga gttattgcgc gatgacgcga tgatgagtgc ggttggttta
    16441 agcctgacaa ttgctgcctg tgcggcaacg gcggcggcga tcctcgggac tattgcggcg
    16501 gtggtgctgg tgcgttttgg caggtttcgc ggatcaaatg gttttgcctt tatgatcacc
    16561 gcgccgctgg tgatgccaga tggcatcacg ggcttgtcgc tgttgttatt attcgtcgcg
    16621 cttgctcatg ccattggctg gcctgcggac cgcggtatgc tcaccatctg gctggcgcat
    16681 gtcacttttt gtacggctta tgtggcggtc gttatttctt caagattgcg ggaactggat
    16741 cgctcgatag aagaagcagc gatggatctc ggtgcgacgc cgctgaaagt gtttttcgtc
    16801 attacgctac cgatgatcat gcccgcgatc atttctggct ggttactggc ttttactttg
    16861 tcgcttgatg atctggtgat cgccagcttt gtttccgggc cgggagccac cacgttaccg
    16921 atgctggtct tttccagcgt gcggatggga gtgaatccgg aaatcaacgc ccttgcaacg
    16981 ttaattctcg gtgcggtcgg aattgtcgga tttatcgcct ggtatctgat ggttcgcgca
    17041 gaaaaacagc ggatacgcga tatccagcgt gcaagacatg gctgaagaca ctaaaatttg
    17101 ccaacctggc tacataatgc cgcgcatgcc gcggcattgt tttcatggaa gacgaaacgt
    17161 tgggattttt taagaaaaca tcttcatctc atgctcgcct gaatgtgcct gcgctggtgc
    17221 aggtggcggc gctcgccatt attatgatcc gtggcctcga cgtgctgatg attttcaata
    17281 cgctgggcgt gcgcggtatt ggcgagttca ttcatcgcag cgtacaaacc tggagtttaa
    17341 cgctggtctt tttaagcagt ctggtgctgg ttttcattga gatctggtgt gcgttttcac
    17401 tggtgaaagg gcgtcgctgg gcgcgctggc tatatctgct gacacaaatc accgccgcaa
    17461 gttacttgtg ggcggcttcg ctggggtacg gttatccgga gctgttcagc attcccggtg
    17521 aatcaaaacg tgaaatcttc catagcctga tgctgcagaa gctgccggat atgctcatcc
    17581 tgatgctgct gttcgttccc tcgaccagtc ggcggttctt ccagttgcaa taatgtgtat
    17641 aatcgtcgcc cctgatgatg tgaaggtcaa tgtatgcagt gcgcacttta cgacgcgggt
    17701 cgctgtcgtt cctgtcagtg gataacgcag ccgattccag agcaactctc cgctaaaacc
    17761 gccgatctta aaaatctgct cgccgatttt ccggttgagg aatggtgcgc gccggtgtca
    17821 ggcccggaac aagggtttcg taataaagcc aaaatggtgg tgagtggtag cgttgaaaaa
    17881 ccactgctcg gtatgctgca tcgcgatggc acgccggaag acctttgtga ctgcccgctt
    17941 tatcctgcct cttttgcgcc cgtttttgcg gcgctaaaac cgtttatcgc ccgtgcgggg
    18001 ttaacgccct acaacgtggc gcgtaagcgt ggcgaactga aatacattct gctgactgaa
    18061 agccagagcg atggcggcat gatgctgcgc tttgtactgc gttctgaaac caaactggca
    18121 caactgcgta aggcgctgcc gtggttacag gaacaactac cgcaactgaa agttattacc
    18181 gtcaatattc agccggtaca tatggcgatt atggaagggg agacggagat ctacctgact
    18241 gaacaacagg cgctggcaga gcgttttaat gacgtaccgc tgtggatccg tccgcaaagt
    18301 ttcttccaga ctaatccggc ggtcgccagc cagctgtacg ctaccgcgcg cgactgggta
    18361 cggcagctgc cagttaaaca tatgtgggat ctgttctgcg gtgtgggtgg ctttggttta
    18421 cactgcgcga cgcctgacat gcagttaacc gggatcgaaa ttgcatcaga ggccattgcc
    18481 tgtgcaaagc agtcagccgc tgaactgggc ttaacgcgtt tgcaatttca ggcgctggac
    18541 tccactcagt ttgccaccgc tcagggggat gtgccggagc tggtgctggt taacccgccg
    18601 cgccgcggca ttggtaaacc gctgtgtgat tatctctcaa cgatggcacc gcgttttatt
    18661 atctactcca gctgtaacgc ccaaaccatg gcgaaagata tccgcgaact gccaggttac
    18721 cgcatcgaac gggtacagct ttttgatatg ttcccgcaca ccgcgcacta tgaagtgatg
    18781 acgctgctgg tgaagcaata aaaaagccgc aggtgcggct tcagattgct gatcaggtgc
    18841 gctttgttca tgccggatgc ggcgtgtacg ccttatccgg cctacaaaat catgctaaat
    18901 caatacattg cagagttgat gtgggccaga taagcgcagc gcatcaggca atcttacttt
    18961 tgtcatcagt ctcaattact gcgggaacca ctggtcattg attttctgat atgtaccgtc
    19021 agctttaatt gctgccagcg cgttattcag tttttccagc agagctttgt tatccggacg
    19081 tacagcgatg cccagaccgg tgccgaagta ttgcggatcg gtcacttttt cagtagcaac
    19141 gcccagttgc ggattggttt tcagccattc gtttaccacc gctgtgtcac caaatacccc
    19201 atcaatacga ccatttttca gatcgataaa ggcattctga taactgtcat aggagacagt
    19261 tttcacttcg gggtgctgat cctgaatata tttctggtgc gtggtgccgt tttccatccc
    19321 aatacgtttg cctttcagat cggcaaacgt tttgtaggta tcttttttgg caatcacgac
    19381 ggctgagttt tcatagtagg gcgtggtaaa cgatacctgc ttgctacgct cgggcgtgat
    19441 atccataccg gagattacgg agtcatattt tctgaatttc agtgacggga tcaggctgtc
    19501 gaacgcgtga ttagtaaaag tacattctgc ctgcatttgt ttgcacaagg cttttgccag
    19561 atcgatatca aagccgacaa tctcattatt agcacctata gattcaaagg gtggataggt
    19621 ggctgaaacg ccaaaattga ttttctctgc ggcagaagca ccgaaagtaa aggaagcaag
    19681 taaagcggca agaactaact ttttcatgat ggaactcccg tctgtcaatc ttatgatttt
    19741 tggccgtgtc tgcggcatgg gataacaatg ccatcaagtg aatttatatg caataaatat
    19801 gattaaataa tttaaatgaa ataaaaaaag acggataact tagtgagttg tccgtcttca
    19861 ttataagaat ttatgcacta tgtaggccgg ataaggcgtc cacgccgcat ccggcacagg
    19921 caccgtgctg atgtctgatg cgacgctggc gcgtcttatc agacctacaa acccccccgg
    19981 cgaatgtacg cagctacatt aattccgccg ttcgaatgcc agtgctttgc gctcgatcag
    20041 acgcatcatc agcgtcagca ggccgttgac gaccaggtaa ataatccctg ccgcaccgaa
    20101 caccattaca tcgtaggtgc gtccgtacaa caactggctg tatcccatca cttccatcag
    20161 cgtaatggtg taagccagag aggtactttt gaacaccagc accacttcgt tggaataaga
    20221 agagagcgag cgtttaaagg catacggcag caggagcgcc agcgtatctt ttttgctcat
    20281 tcccagggcg ctacaggact gccactgacc ttctgggatc gcacgaattg caccgtaaaa
    20341 cagctgcgtg gtatacgccg cactgttcag cgataacgca atcagcgcac ataaccacgg
    20401 ttctgacaac aaatgccaca gtgccggata ctcctgcaac gtcggaaact ggcccggccc
    20461 gtaataaatc aggaagatct gcaccagcag cggcgtaccg gtaaacagcg tgatataacc
    20521 ccgcaccagc cacaccagca ccggcgtttt cagcgtcagg atgatggtaa aaatcaatgc
    20581 cagaatcagt gccacaatca gcgaggcaac ggttagcgtc aggctggtgt gtagcccttt
    20641 catcagttcg ggtaaatact caaacattag ctgggcctcc gctcaaaacg tgtcgcgcgc
    20701 aggtcaatgc gtttgagaat gtactgactg agcagggtga tcaccaggta aatcgccgcc
    20761 gccacaatgt accaggtaaa tggttcctgg gtacgagtag cgatgctttt tgtttgcagc
    20821 attaaatcat tcacactaat caaactgacc agcgcggtat ctttcagcag caccagccac
    20881 tggttaccga ggccaggcag cgcatgacgc cacatctgcg gcatcaccag acggaaaaag
    20941 atagccgatt tcgacagccc cagcgcctga ccagactccc actgacccac cggcaccgct
    21001 ttcaacgcgc cccgaagcgt ttgcgaggca taggcggcat acagcagtga cagagcgatg
    21061 acaccacaaa ggaacgggct aacgtcgaag ttctcaatgt ccatctgcac tgggatctgc
    21121 acgaacccaa gattgatagt gaagccatcc gaaagcgtca gcagcagctg cgaggagcca
    21181 aaatagataa acagcaccac cagaatttct ggcaggccac gcagaatggt taccagcgct
    21241 gaacctgccc acgcgacagg acgccatttt gccgactccc ataccgcaaa gaacatcgcc
    21301 agcgccagcc cgacaatcaa tgcacaaacg gcaaggccga cggtcatccc ggcggcgctt
    21361 gctaaaggaa aaaattcatt catcaggaat tacttctgga accatttgtt gtagatggtt
    21421 tcgtaagtgc catctttctt cactttttcc agcgcagtgt tgagtttctg ctgcagctca
    21481 gtgttgccct gacgtaccgc gatgccgagg ccagtgccga agtaatcttt atcggtcact
    21541 ttgtcgccca ccgccgccag tttcgggtta tctttcagcc actcagtgac cactgcggtg
    21601 tcaccgaaga cgccgtcgat acgcccgttt tgcagatcca gttttgcgtt ctggtagctg
    21661 tcatacggaa cggtagtgat ttccgggtgc ttatccataa tgaatttctg gtgtgtcgtc
    21721 ccgttctgta cgccgacttt tttgcctttc agctgatcaa cactggtgta tttgccttgc
    21781 tgacccacaa acagggcaga gttgtcatag tacggggtgg taaacagcac ctgcttttca
    21841 cgctccggag tgatatccat gcccgccatc acggcttcta cgcgacggaa tttcaggctt
    21901 gggatcaggc tgtcaaacgc ctggttagag aaagtgcagg ttgcatcaat ctctttacac
    21961 agcgcttgtg ccaggtcgac gtcaaaacca acgatctggt tgtttgcatc aatcgattca
    22021 aacggaggat aggatgcttc ggtagcaaaa cgaatggttt cggcagctgt ggcggaaaga
    22081 ctaaaacctg caattaacgc ggcaatcaga acttttttca ttgttgttat cccgaatctt
    22141 agtgagagag atagttttta aatgcttcgg tttgcggctc ggtaaagcag ctcgcgtcgc
    22201 cttgttctac gatatgacca ttttccatat acaccactcg gctggcggtt ttacgcgcca
    22261 cttcaacttc gtgggtgacg atcacctggg taatattcgt ttctgccagc tcacgaatga
    22321 tgctgacgat ttgtgccgta atttccgggt ccagtgcggc ggtcggttca tcgaacagca
    22381 gtacctgcgg ttccatcatc aacgcacggg caatagcaac acgctgctgc tgaccaccag
    22441 aaagatgcag cgggtaacga tcgctataag gtttgagacg cagacgttcc agcagttttt
    22501 ctgcacgggc cagcgcctga tctttactca accccagtac acggcagggc gcttcaatca
    22561 ggttttgctg cacggtcaga tgcggccaca ggttgtattg ctgaaacacc atgccaacgt
    22621 tacgacgcaa atcgcgaatc gctttgtcag agggtgtttt ggtgaaatcg aaatggttgc
    22681 ctgcaatgtt gagcgtaccg gagcgtggca tctcaagcag attgagtaca cgcagcagcg
    22741 agcttttacc cgcgccgctg gggccaagta acaccagcgt ttcgccctgt gggcaatcca
    22801 gcgtgatatc gaacagcgcc tgatgcgcgc cgtagaagca attaatgccg tttaattgaa
    22861 tactcattga cactcgtata ctggcagtct gatagctatt gaggtcgaag atagtacctt
    22921 tgacagaata attatgcaat atttctgctt taaaagttaa aagcaaagcg cattattcaa
    22981 taaacatagc acaaaataac gggggcggtg gtcggcgagc ataaatgtcg gcattcctca
    23041 cgaaatgccg gacaatttac ggggtttatt ggttgatcaa ggcgttagcg attctcgatg
    23101 gactgacgga gcgtacccgc cgtggcatga acgctgccgc ctaagtagcg cacatcgtcg
    23161 atgacccaac actggccttc ctgaatcatt aacacttcat cctgccaacc ctggtcaccc
    23221 tgtttgagat ctacgcgcaa tggaatgtta cgggcatcac gattagggat agtcgatgca
    23281 ctggcaacgt gggcgctatc tggcaaggtg gttcgactgg agaacggatc gctgcttaac
    23341 aattcacgat gactattatc gcggctggca tcgctaagaa gggttgccag tttgtcactc
    23401 aaataggggc gcagggcggt aatgtcattg ctgcgatgtt gaatgcggta gtcatagaat
    23461 tgttgagcca cgctgtcagg ccccccctgg acacaggaac cggtgcgggg accgttatct
    23521 ttataagctg gcgtgacggt ggtacaggca ctcagcagca gtgcacatgg aataagtaga
    23581 gatagtgaac ggtagcgcat aatcatttcc ttattagctt gctgtctgcg tcacactacg
    23641 accaaacgct atcagattat ttaatacatt gtttttaata caattctgaa acaatctgaa
    23701 cacctgattg ggtttgagtc cattgctggc tatccactca aggttgctcg tagctgactt
    23761 acttaaatta cccaaacaat agacagtgta aaccatttgt gaggttgagc gtgttatatg
    23821 gctgaaatga tcatttaagg taaatatgat agttctgaaa tggtggtata aatcctcctg
    23881 tagaggttaa gagcatctgc aagaagccag tttcacattt ataacgcgtg ataaatgaac
    23941 gcggttattg tctaatcatt acaaaatttt tcacatgcga taagagatcc tgcgattaag
    24001 atgattgcca aaattacgtt gtatttttga aaatgagcga aatttgatag tcccagcttt
    24061 gactctcgat aatattattt atgctacaat tgtggctaaa gaatttaagc taatgaattt
    24121 tagcgaagaa taaattaagt tgtttcagta tctcgttaat taatatgaat ataaaaggcg
    24181 ttgtgctttt ataattataa agcacgagga gttcggtgga ttatcgtttt tcttgggtga
    24241 tttttaatat gataaacaat atatctgatc aagcatcatc atttccaggc acacaactaa
    24301 atcaaagtga taatttcctg gattcactga gggaattttt cgcgatatta aattccagtc
    24361 gtaagggtga gttgtctaca tgggatacca tctacttgca tctgatatta gcaataaatg
    24421 ctgacagtga tctcataaaa aatgatgtgt tgctcgcgga aaatatgcca tcagcgaatt
    24481 accaatttaa tacttttttt agtaatgctt tcgagactga tgttaaaaaa tatctgggta
    24541 aatcagaaga taacgaagtt gaaataaagg cgggtaatga aagaatctct attggaatac
    24601 gtaatgtatc gaatgggaaa cttgaaagac aacagtttct tttccctctg gactatgaga
    24661 ataaattaca agatcaatta gacaagcatt ttacgattga atcgcaccca ttattataca
    24721 ggcataccat tggtagtaaa attgcgaatg tgatttttga aaaattatac agccgtattg
    24781 actttaacaa agaacaatat atatccttta taaagggcgc gtttattcat ttttatgatt
    24841 atagcaggcg atatgcaatt agtgagaata tcgaaaaaga cgctgtaaca aacaatatcg
    24901 cattgatgaa cactttttat gacagtgata atacgtcagg tgaggtttta aataatgatt
    24961 ttacagaaga agagccattt gaaactgcgt tagatgtaga gcatacaata gtgcttggat
    25021 ttgctgatga taattttgaa acaaaacctg ttcattatca ggatttatta acacgattta
    25081 gtgcctttca agataccgtg tttaatctgt ttcctgaaat gcgctcttcg cattaccacg
    25141 atatttgtag cgtatgtgtg gatatgacaa agggaacgca atgcatgatt cacttgatgg
    25201 tgaatgaaga agttttcatg tctctgcctg ttccagttgc cacgatggta cgtgaagatg
    25261 caagtaattt agtcaatctt aaaacactac tcaatgatgg ttttttcatt aaatatagtc
    25321 attttaatga cgtggcatta ataaagcaaa acataagtaa tctctactta tcacacactg
    25381 ttgtgaatga aagcatattg aaaaaatgtt gttttgaaaa cggcagttta ggtgatgtta
    25441 aaataacaaa tagcaatata ataaataatg tattcaaaaa tgtttcgttt agatctgtta
    25501 aaattaacaa cgtaaatact cattctttaa aatttattaa ttgccatttt tttaatgttg
    25561 atatgataag agttaatttc agcaaatgtc tttttcatga ctgtagtatg catggtgtca
    25621 agataaaacc atggttgcca gtaaaatgga caaaagagtt aataaatgat tatttgtatg
    25681 gctgtttact atctctgtac tctatctgtg caagggatat atataatatg agcgccggga
    25741 ataacgtgaa ggttgctgcg gatgctttcc ttgaaattat tttctctctt aagaataaat
    25801 attgcattaa actattaagc gctcaggaac gggcgtttat ttacgaattt gcaagaatga
    25861 tcttcgctta tattaatgac aaaagcattg agatcttatt actttcttgt tttgcggcgt
    25921 ctgaccaaaa agcaatccag aggtatatac ctcagtcaca agatggggaa gactttagga
    25981 gtcatttaca atataagttg cctctttccg ctcattaatg ttgttggtgc aacataataa
    26041 gacaggtaag cggtacccta taatgaaacc aatatcgggt tatgcaacgg ataatcgtaa
    26101 agacttaagt gggtgttttt gacacaatga gatgatttcc taaactgatc aaaaccttct
    26161 tcccaacatg gcatatcagt tcgataatgc tttagagatc gaaggcttaa caaacaagga
    26221 gatcgatcat gcaattttct acaaccccaa ctctggaagg ccagaccatc gttgaatatt
    26281 gcggtgtggt gaccggcgaa gcgattttag gtgccaatat tttccgtgat ttctttgccg
    26341 gtatccgcga tatcgttggc ggacgttccg gtgcgtatga aaaagaactg cgtaaagcac
    26401 gggagatcgc ctttgaggaa ttaggctccc aggcgcgggc gctgggggcc gatgccgtcg
    26461 tcggtattga tatcgactac gaaacggtcg ggcaaaacgg cagtatgctg atggttagcg
    26521 tcagcggtac ggcggtgaaa acgcgtcgat gagaagagtc ttctggctgg tcgctgccgc
    26581 tctgttattg gcagggtgtg caggcgaaaa aggcattgtc gagaaagagg gatatcagct
    26641 tgatacccga cgccaggcgc aggcggcgta tccgcgcatt aaagtgctgg tgatccacta
    26701 caccgcagat gattttgata gctcgctggc gacactgacc gataagcagg tcagctcgca
    26761 ttatctggtc cctgcggtac caccgcgata caacggtaaa ccgcgcatct ggcaactggt
    26821 gccggaacaa gaactggcct ggcatgcggg gattagcgcc tggcgcgggg caacgcgcct
    26881 taacgacacc tctattggca ttgagctgga aaaccgtggc tggcaaaaat cggccggagt
    26941 gaaatatttt gccccgtttg aaccgacaca gattcaggcg cttattccac tggcgaaaga
    27001 tattattgcc cgttatcaca tcaagccgga aaacgtagtg gcacatgcgg atatcgcacc
    27061 gcagcgcaaa gacgatccgg ggccattatt tccctggcag caactggcgc agcaggggat
    27121 tggtgcctgg ccggatgcgc agcgggttaa cttttacctt gccgggcgcg cgccgcacac
    27181 accggtagat accgcgtcat tgctggagct tttggcgcgc tacggttatg acgttaaacc
    27241 tgatatgaca ccgcgcgagc agcggcgcgt gattatggca ttccagatgc atttccgccc
    27301 gacgttatat aacggcgaag cggatgcaga aactcaggcg attgccgaag cattgctgga
    27361 gaaatacggg caggattagc gcggcagttt tccgtggtcg cgtagccagg cggcagtttt
    27421 ctcgatacct tcatctaggg tgatgatcgg ctgataacct aactcttcct gcgcacgcgt
    27481 aatatccagc gtaaagtcaa aattcaactt ggagacgccg tagtgggtca gcggcggctc
    27541 ttttgctgac ttgcggccta aacgctccat gctgcgggcg atcatatcca gcatcgggta
    27601 ggggacggaa cgaatacgac agtcaatatt caactcgtcg atcagcttct gcacgatgct
    27661 gcgcagtgtg cgatgctcgc cgttggtgat attgtacaca cgcccggaag gtagcttatc
    27721 gcaggcttcc tggcttgcca gccacattgc gtgcacggta ttttcatagt aggtcatatc
    27781 gaccagcgca ctgccgccat gcggtaacag aatactgccg tagtggtgca tcatatgcgc
    27841 cagacgggga ataaagactt tatcgtgcgg tccgaacaga ctttgtgggc gcagaatagt
    27901 aaagcgcgtt tgtggattcg cctgcgaaag catattgatc acttcttcgc tggctgcttt
    27961 gctgcgggca aactcgttgg cgaagcggtg agggcgaaaa tcttctttaa tatcgcgatg
    28021 gtggtgataa tcgaagtaca gggaggggga agagatatga ataaagttac gcacacccca
    28081 ggcgacagcc cattcaccca ggcggcgagt ggcgcgaacg ttagccagat cgaaagcctg
    28141 ttgtgtcccc cagggtgagg taaagctgga gcagtgccac agcgtatcaa tgcccgcgag
    28201 catcacttta gcttgtgatg aaaccagctc ggtcagatcc gccggaacaa actctgcgcc
    28261 cattttttcc agcaatttgc ccattgcctc gttgcgaccg gtcgctcgca cgctgatgcc
    28321 tttctggcat aaaaactcta ccgcgtttcg acctaagccg ctggtggcgc cggtaaccag
    28381 taccttcata tcaatccact gttgttgaga aaatgacgtg cgcattcttc cgtgatttcc
    28441 cccatgatgc aatgggaaac atgaaagaat aacgcaggtt ttgtcgatta atctgtgctt
    28501 tgttctgcca gtctggcgat ttgttttgcc attccgcgaa aaataaacag atgcgcgggg
    28561 atcatcaata accagtaaaa cagccccggc ataccgtgcg gatgccagaa agcgcggaca
    28621 tcgatagtac gatagtcgcc tttatcttcc aggctaaaac acagtcgtcc cagccccgac
    28681 gctttcatgc caaataacaa cgtaagttgt ttttccggtt caacgacaat cactttccag
    28741 ctatccaccg catcgccagt ctgtaaatat tcgcgctccg ggcggccttt cgccagctta
    28801 tgaccgatcg cgcggtccat caacgcccgt gtctgccaca aaatattgcc aaagaaataa
    28861 cgctctttac cgccgatttg gttcactacc tgccataaag cagcaaggct ggcggacgtt
    28921 ttaacggtaa accccgcctg tttggcaaaa taaccgtact ccggtcgcca gcgggcaaag
    28981 gcctgagcgt cgtagcccca gtcgctggag ttgaccagtt tttcctcctc tttcaacgtg
    29041 ctacgtaccg cgtcatcgaa agcgatcagc cgttgtggga tgagtgcacg tagcgcggta
    29101 tcatccgcca gcagatcgtg tttcagcccc tgaatcaacg ccctggcggt ggtgggcggt
    29161 acggaagtaa tcacattgag aaaccacacc gaaatccagc gggtggggag ggggatgggg
    29221 atcaaccagc ggcgcttacc gctcaccgcc ataaaatgtt caaactgttg ctgataactg
    29281 agcacctctg gtccggcggc ttcgaagatg cggtgttcgc tggccggatg atctaacaac
    29341 gccaccagat agtgcagcaa gttttccagc gcgatgggcg tggtgcgtga acgtacccag
    29401 cgtggcggcg ttaacactgg caggttgtag accatatcgc gcatgacttc gaacgccgct
    29461 gaacctgcgc caacgataat tccggcccga agttcggtca caggtacatt cgcttcacga
    29521 agaatgtccg ccgtagcctg acgagcacgc agatgatccg actgctcatg tggcggggcc
    29581 tgcaacgaac tgagaaagat taattgctta actggtactt cacgtagcgc atcgcggacg
    29641 ttgagagcca cctggcgctc ctgagcgata aaatcgccgc cttcgcccat gctgtgcacc
    29701 agaaaataga cggtatcgat atcctgcaac agggccggaa ggttatccgg ccagctgaga
    29761 tcgactttat ggcaactgac gtttgccagt tgcagctttg caagcctgtc gacatgacgt
    29821 gccgccgcca ggatctgatg cccttgctgg ctgagtgtgc gcaccagatg ctgaccaatg
    29881 tagccactgg caccgagaac taaaatgcgt tgcggcacgt ctctctcctt aacgcgccag
    29941 gaatgcgcgc cagtgggcgg cgacttccgc cagttgttcg cgcgagacgt caagatgcgt
    30001 caccaggcgg acaatcggcg aggcgttaat cagcacgttt ctcgctttca tgtattcgcc
    30061 taacgcggca gcattttctt ccccgacgcg aacaaacagc atattagtgt cctgacgcat
    30121 cacatccgcg cctgcttcac gcagttgctc cgccatccag gcggcgttgt cgtgatcttc
    30181 ctgcaaccgt gcgacgttat ttttcagggc atacatcccg gcggcagcca gaatcccgga
    30241 ctgacgcatc ccgccaccgg ccattttccg ccagcggatg gcacgtttaa tgtaatcacg
    30301 attaccgacg agtaatgaac cgactggcgt cccaagacct ttcgacaggc aaatggtgaa
    30361 cgaatcacaa tattgcgtga tctctttcag ttcgcagccg taagccacca cggcattaaa
    30421 gatgcgcgca ccgtcaacat gcagcgccag attgcgcttg cgggtaaatt cccatgcttc
    30481 tttcaggtat tcacgcggca gcactttgcc gttgtgggtg ttttctagac tgagtaattt
    30541 ggtgcgggcg aaatggatat cgtcgggttt gattttcatc gccactttat ccagcggtag
    30601 cgtgccgtcg gcagccgcgt cgatgggttg cggttgaata ctgcccagca ccgctgcgcc
    30661 accggcttca aacagataat tatgcgcggc ctgaccgaca atatactctt cgccgcgttc
    30721 gcagtgactg agcagagcga ccaggttagc ctgggtgccg gtcggcagaa aaatggcggc
    30781 ttctttaccg gaaagctctg cggcgtagtc ctgcagagca ttaacggtag ggtcgtctcc
    30841 gtaaacgtcg tccccaaccg gggcggccat cattgcttcg agcatggcgc ggctcggtcg
    30901 ggtaacggta tcactgcgta aatcaatcat ggcatgtcct tattatgacg ggaaatgcca
    30961 ccctttttac cttagccagt tcgttttcgc cagttcgatc acttcatcgc cgcgtccgct
    31021 gatgattgcg cgcaacatat acaagctaaa tcctttggcc tgttcgagtt tgatctgcgg
    31081 tggaatggct aactcttctt tggcgaccac cacatccacc agcaccggac cgtcgatgga
    31141 gaaggcgcgt tgcagggctt catcaacttc agacgctttt tctacacgga tacccgtaat
    31201 gccgcacgct tcggcaatgc gggcaaagtt tgtgtcgtgt agttcggtgc cgtcagtcaa
    31261 atagccacca gctttcatct ccatcgccac aaagcccagc acgctgttgt taaagacgac
    31321 aattttcact ggcagtttca tctgcactac tgagaggaaa tcgcccatca acatgctaaa
    31381 accgccatcg ccgcacatgg cgaccacctg acgttctggc tctgtcgcct gcgcacccag
    31441 cgcctgcggc atggcgttag ccatcgaacc gtggttaaac gaacctaaca ggcgacgctt
    31501 gccgttcatt tttagataac gtgccgccca caccgttggc gtaccaacgt cacaggtgaa
    31561 aatagcgtca tcggcggcaa aatgactaat ttgctgcgcc agatattgcg ggtgaatggc
    31621 tttctcgctc ggtttagcta aatcgtccag ccctttgcgg gcgtcgcggt aatcttccag
    31681 cgctttatcc agaaacttgc gatcggcttt ttcttccacc aatggaagca atgcacgcag
    31741 agtcgacttg atatcgccga ccagtgccat atccaccttg ctgtgagcgc cgatgctggc
    31801 tgggttgata tcaatctgaa tgattttggc atcggtcggg tagaaggcgc ggtagggaaa
    31861 ttgcgtgccg agtagcacta acgtgtcggc gttcatcatg gtatggaaac ctgacgagaa
    31921 gccgattaac ccggtcattc caacatcata cggattatcg tattcgacat gttctttacc
    31981 gcgcagggca tgaacaatag gcgctttaat tttcccggca aactcaacta actctttatg
    32041 cgcccccgcg cagccgctgc cacacatcag ggcgatattg ctggaataac gcagcagttg
    32101 cgccagtttg cgtaactctt cttcttccgg cgtcacgact ggttgtggcg catgatacca
    32161 gtgcgtggtt gccccttctg gcgcaggttt taacgccacg tcgcctggta acacgacaac
    32221 cgaaacgcca cggttaagca ccgctttgcg catggcaatc gccagtactt gtgggatctg
    32281 ctccgggctg gaaaccagct cgcaatagtg actacattcg cggaatagct cttgtgggtg
    32341 ggtttcctgg aaatagccgc tgccaatttc gctggaggga atatgagcgg caatcgccag
    32401 taccggaacg tgattgcggt ggcaatcgaa caggccgttg attaagtgca ggttgccggg
    32461 gccgcacgat ccggcgcaga ccgccagttc tccgctaagt tgtgcttcag cgccagcggc
    32521 aaaggccgcc acttcttcgt ggcgggtgga catccactcg atggtgccca tgcgattaag
    32581 actgtcacta agaccgttca gagagtcgcc tgtgactccc cagatgcgtt tcacccctgc
    32641 cgattcgagt gttttggcga tataagctgc aaccgtttgt ttcatggttc tccatctcct
    32701 gaatgtgata acggtaacaa gtttagttca tctgacggag ggggaaggga tgggagagaa
    32761 aggaggcact aacggttaaa tagcccgatg aaaggaatat catcgggcat aaggcgatta
    32821 tgcgagaacc aaatccccct gcggatggca ggagcaggcc agtacgtaac cttcagcgat
    32881 ttcggcgtcg gtcagcgtca ttgtactgct caccgtatat tcaccggaaa ccacttttgt
    32941 cttacagcag ccgcaaacac ccgcacggca ggcagcgaca accggaacgt tattgctttc
    33001 cagcgcctcc agtagcgtgg tgccaaccgg ggcgtaaaat tctcgtgccg gttgcagttt
    33061 ggtgaatttc agaccgctgg tcgccgcttc tgctactgga gtgaagaatt tctctttaaa
    33121 gaaacgcgtc acgccgagcg ctttcacttc ctgctctacc caatccatat acggagccgg
    33181 gccgcaggtc atcacggtac gtgaagctaa gtcaggtaca cctgccagca gttcgcgagt
    33241 gagacgacca gcgataaagc cttcggtaac gttattttct gccaccagcg ttaccggata
    33301 gttacgccac tcatcggcga aaataacatc ctgcggcgta cgcacgttgt agatcacccg
    33361 cacatcggcc tgtggacggt tcttcgcaag ccagcgacgc atcgacataa tcggcgtgac
    33421 gccgcagcct gccgccagca acaggaattt atcttctgct ttatcgtcgc aggtaaattc
    33481 ccccatcgcg tccgaaagcc agagataatc accgcgtttt acatcgcgcg tcagccactg
    33541 ggagccgaca ccgtcatcaa tccgccgcac ggtcagggtg atatattcac tcacgcctgg
    33601 cgtggaggaa atggtgtaag cacgcagcgt ttccgctgag tttcgcacgc tgaccagtgc
    33661 atattgcccg gcgcgatatg ggtagtaatc gtggcaaatc agggaaatcg tccacacatc
    33721 cggcgtttct tgcgtaatgt gatgaacctg catccgccac gggcattgat tcgttggcat
    33781 cgtcatcgac aaactcctta cgcgctcaac agttgcttca tgtcttcttc aacagtggtg
    33841 atagaacgca ggccgaattt ctcgttcagc accgccagca ggtctggtgt caggaaacca
    33901 ggtgcagtcg ggccggtgac gatatttttc acgcccagag aaagcagcgt cagcagaatg
    33961 acgatcgctt tctgttcaaa ccaggagagc accagcgaca gcggcagatc gttcacacca
    34021 cagcccagtt tctctgccag agtgacagcc agaataatcg ctgagtaagc atcgttacat
    34081 tgacctgcat ctaccagacg cggcagacct tcgatatcgc caaactcaag tttgttaaag
    34141 cgatatttac cacaggcgag ggtcaggatc aggcagtcat ccggcacgct ggtggcgaaa
    34201 tcggtgaagt agtggcgctc gccgcgtgcg ccgtcacagc caccaagcag gaagatatga
    34261 cgcagttttt cacggctcac cagatcaatc agcgtatcag cagcgccaag cagcgtctgg
    34321 cgaccaaaac ccacggtgat aaggtgcgga atttcgctgt acgggaagcc tgccatctgt
    34381 tgcgcctggg tgataaccgc agagaaatca tcaccgtcca gatgacgcac gccaggccag
    34441 ccaacaatgc tgcgggtcca gatacgatcg tcataagcgc ctacggttgg gtcgatgatg
    34501 cagttcgagg tcatcacgat ggggccaggg aaacgagcga actccacttg ctgattctgc
    34561 cagccgctgc cgtagttacc gaccagatgc ttgaatttac gcagctccgg gtagccatgc
    34621 gcaggcagca tttcgccgtg ggtgtagaca ttaacgcccg tgccttcggt ctgttccagc
    34681 aggttgtaga gatctttgag atcgtgaccg gaaatcagaa tgcatttacc cgccgtcgct
    34741 ttgacgttga cctgggttgg cgtcgggtga ccgtatttac cggtttcgcc tgcatccaga
    34801 atgctcatca ctttgaagtt catctggccg atttccattg aacactcaag aagcgcgttc
    34861 atatcggcag gccaggtccc cagccacgcc atgattttat ggtactgggc ataaatatcg
    34921 ttgtcgtatt gaccgagaac atgcgcgtgt tccatatagg ccgccgcacc tttcaggcca
    34981 tacaggcaca gcagacgcag gccgagaatg ttttcgccaa tcgccgcttt atctttgtta
    35041 ggggtaaatt ctgctgcctg acgttgcagc tcgccgagat catcgctcac cagttgcagg
    35101 tcagccatcg ggttatcgac gcgcgcgttg gcatctacag ccaggcattg tgctttcagc
    35161 gcctcgcgca aggcaatcgc ttcacgagcg tagccgacaa tacgcggaga atcgaagtta
    35221 acgttggtca gggttgagaa aaaggcacgt ggcgcgaagc tgtcaacatc gtggttgatg
    35281 atgccgtatt cacacgcttt taccgcccag gcagaaagcc cttgcagcgc cgcgatgagt
    35341 aaatcctgaa ggtcagaagt ttccgccgtt ttaccacaca tcccctgcgc gtatgagcag
    35401 ccgtttcctg ccggagtacg gatagtttgt tcacattgca cacaaaacat gatcacacct
    35461 tttaaagtta tatttaatat acatgtttaa ggttaagacg cttaacgcgg ggataaaagg
    35521 gatttttcat gcaactttaa gggagattga tttagcgcaa ttttggcggc agggatctac
    35581 cgccagagag gtattacgca gagaaaaagg cgatgaggat cggcactaac aggctaagaa
    35641 taaaaccgtg aacaattgcc gccgggacca tatccagccc gccagtacgt tgaagaacgg
    35701 gcagggtgaa atccattgat gtggcaccgc ataagcccag tgcagtagag cggctgcggc
    35761 gaatcagccc agggatcaac ataatagcaa tcagttcacg ggccagatca ttaaaaaacg
    35821 ccgcgctccc gattaccgga ccaaaagatt cggtcaataa aataccggaa agagaatacc
    35881 agccgaaacc ggaggccatt gccagcgcgg tattgatggg gagatcaaga ataaaggcgt
    35941 taattaaacc accaattaat gaactgacaa ccaccaccac ggcgacaatc attccccggc
    36001 gattaaggac aatctgcttt aaggtcatgc cattattgcg caactgaata ccaacgagga
    36061 aaagtagcaa aattaacgtg tattcactgg cttcggtcgc gtgttgtaag aaagccagtc
    36121 cactcagacc aatggcaaaa ccaatcacta ctacgccgca cagttttagc gactccagcg
    36181 ccatcgcaat acgcgacggg agtttttctt gctgatggtg gttgcgccac ggcaggcctc
    36241 gctccagcca catcagggcg gcaatattac acagtaaaat aacggtaata ctgacggcag
    36301 aataatgcag aatcgccaac aggttactgg cgaggttatc gagaaacgcc agactgatac
    36361 ccataaaaaa gagaataagg taaaccatcc agcttaatag ctgattaata acttttaacg
    36421 cagcttgttg gcgaagcgga atgaggtaac ctacaatcag gggaaccaga atgattaaca
    36481 gcccagaaaa catgaaaacc cagtccttgc aaagatgaag tcgaaatgcg cgatgacaca
    36541 ctactgaaag cggaaggacg agtaaagttg caattaaaag gaaatgttat gcataaggag
    36601 cagtagagta ttcgttttca tttaaagata ttcttgcgct ttaattacaa actgcaccga
    36661 tgttggtggc gtcaaaatcg ccgaggcgtt ccctgaaggc cggggcagcc cacatg
//
LOCUS       DAJTZL010000022        36024 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.022, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000022 DAJTZL010000000
VERSION     DAJTZL010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36024
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.022"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            188..1003
                     /gene="mepH"
                     /locus_tag="OO544_000951"
     CDS             188..1003
                     /gene="mepH"
                     /locus_tag="OO544_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HCR6766615.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNKTASA
                     SFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPYRWGG
                     SSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFFRTQG
                     RGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            1131..1712
                     /gene="sodB"
                     /locus_tag="OO544_000952"
     CDS             1131..1712
                     /gene="sodB"
                     /locus_tag="OO544_000952"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HCR6766616.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(1858..3027)
                     /locus_tag="OO544_000953"
     CDS             complement(1858..3027)
                     /locus_tag="OO544_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6766617.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGTGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLATPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(3193..3282)
                     /gene="ynhF"
                     /locus_tag="OO544_000954"
     CDS             complement(3193..3282)
                     /gene="ynhF"
                     /locus_tag="OO544_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein YnhF"
                     /protein_id="HCR6766618.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            3581..4606
                     /gene="purR"
                     /locus_tag="OO544_000955"
     CDS             3581..4606
                     /gene="purR"
                     /locus_tag="OO544_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HCR6766619.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLVGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(4603..5535)
                     /locus_tag="OO544_000956"
     CDS             complement(4603..5535)
                     /locus_tag="OO544_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6766620.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     VVVPDWESSATCISAGLCIGMVPIHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            5648..6859
                     /locus_tag="OO544_000957"
     CDS             5648..6859
                     /locus_tag="OO544_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HCR6766621.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGMTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIIPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEAGCQNH
                     GNAEVAHSESH"
     gene            7150..8298
                     /gene="cfa"
                     /locus_tag="OO544_000958"
     CDS             7150..8298
                     /gene="cfa"
                     /locus_tag="OO544_000958"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HCR6766622.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(8338..8979)
                     /gene="ribE"
                     /locus_tag="OO544_000959"
     CDS             complement(8338..8979)
                     /gene="ribE"
                     /locus_tag="OO544_000959"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HCR6766623.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            9194..10567
                     /gene="mdtK"
                     /locus_tag="OO544_000960"
     CDS             9194..10567
                     /gene="mdtK"
                     /locus_tag="OO544_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HCR6766624.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRMPSAI
                     ILQRASR"
     gene            complement(10608..11864)
                     /locus_tag="OO544_000961"
     CDS             complement(10608..11864)
                     /locus_tag="OO544_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HCR6766625.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPSGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGAELQQSTITVQQGGVLILDGSTVKGDSVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVNGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            12172..12248
                     /locus_tag="OO544_000962"
     tRNA            12172..12248
                     /locus_tag="OO544_000962"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12206..12208,aa:Val,seq:gac)
     gene            12253..12329
                     /locus_tag="OO544_000963"
     tRNA            12253..12329
                     /locus_tag="OO544_000963"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12287..12289,aa:Val,seq:gac)
     gene            12437..12742
                     /locus_tag="OO544_000964"
     CDS             12437..12742
                     /locus_tag="OO544_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HCR6766626.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            12868..14472
                     /locus_tag="OO544_000965"
     CDS             12868..14472
                     /locus_tag="OO544_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HCR6766627.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPINCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFHRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EYGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYKMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(14484..15296)
                     /locus_tag="OO544_000966"
     CDS             complement(14484..15296)
                     /locus_tag="OO544_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6766628.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIVTAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGPGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(15300..16085)
                     /locus_tag="OO544_000967"
     CDS             complement(15300..16085)
                     /locus_tag="OO544_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HCR6766629.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGEKVYLYSKAVRLWHWSNALLFVLLLASGLINHFAMVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGDNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMCGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            complement(16082..16750)
                     /locus_tag="OO544_000968"
     CDS             complement(16082..16750)
                     /locus_tag="OO544_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HCR6766630.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQENKYYQYQLLGAGKPHLYRRFGQHLIK
                     KENV"
     gene            complement(16814..17452)
                     /locus_tag="OO544_000969"
     CDS             complement(16814..17452)
                     /locus_tag="OO544_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HCR6766631.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARTVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            complement(17465..19573)
                     /locus_tag="OO544_000970"
     CDS             complement(17465..19573)
                     /locus_tag="OO544_000970"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HCR6766632.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIRWDQLEAGDVNFIKIKDFYYRLAHRVGELSHLADGSYAIAER
                     WNLGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGL
                     PLKLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPM
                     TVSPLKSRNYRGDLALEAKFFKAITGEEMTQEKLDLAAERIFTLHRAYTVKLMQTKDM
                     RNEHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGCPTRETL
                     QRLGLEDIAADLAAHNLLPA"
     gene            complement(19594..20220)
                     /locus_tag="OO544_000971"
     CDS             complement(19594..20220)
                     /locus_tag="OO544_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HCR6766633.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            complement(20676..20885)
                     /gene="fumD"
                     /locus_tag="OO544_000972"
     CDS             complement(20676..20885)
                     /gene="fumD"
                     /locus_tag="OO544_000972"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HCR6766634.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
     gene            21442..22854
                     /gene="pykF"
                     /locus_tag="OO544_000973"
     CDS             21442..22854
                     /gene="pykF"
                     /locus_tag="OO544_000973"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HCR6766635.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
     gene            23164..23400
                     /gene="lpp"
                     /locus_tag="OO544_000974"
     CDS             23164..23400
                     /gene="lpp"
                     /locus_tag="OO544_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HCR6766636.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(23463..24467)
                     /gene="ldtE"
                     /locus_tag="OO544_000975"
     CDS             complement(23463..24467)
                     /gene="ldtE"
                     /locus_tag="OO544_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HCR6766637.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIVPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(24616..25032)
                     /gene="sufE"
                     /locus_tag="OO544_000976"
     CDS             complement(24616..25032)
                     /gene="sufE"
                     /locus_tag="OO544_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HCR6766638.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLTELRDEDRSP
                     QNSIQGCQSQVWIVMRQNAHGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVSFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            complement(25045..26265)
                     /gene="sufS"
                     /locus_tag="OO544_000977"
     CDS             complement(25045..26265)
                     /gene="sufS"
                     /locus_tag="OO544_000977"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HCR6766639.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEIITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNNIAEYEQNLMHYALSQLESVPDLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            complement(26262..27533)
                     /gene="sufD"
                     /locus_tag="OO544_000978"
     CDS             complement(26262..27533)
                     /gene="sufD"
                     /locus_tag="OO544_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HCR6766640.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGDISPQQRDALALTLDAVRLVFVDGRYVPALSDAIE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPLSHHFAHNDLLLAEDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKMAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            complement(27508..28254)
                     /gene="sufC"
                     /locus_tag="OO544_000979"
     CDS             complement(27508..28254)
                     /gene="sufC"
                     /locus_tag="OO544_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HCR6766641.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            complement(28264..29751)
                     /gene="sufB"
                     /locus_tag="OO544_000980"
     CDS             complement(28264..29751)
                     /gene="sufB"
                     /locus_tag="OO544_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HCR6766642.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            complement(29760..30128)
                     /gene="sufA"
                     /locus_tag="OO544_000981"
     CDS             complement(29760..30128)
                     /gene="sufA"
                     /locus_tag="OO544_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HCR6766643.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            complement(30676..30864)
                     /locus_tag="OO544_000982"
     CDS             complement(30676..30864)
                     /locus_tag="OO544_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HCR6766644.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSTELKEEIYFAWRLGVH"
     gene            complement(30964..31374)
                     /gene="menI"
                     /locus_tag="OO544_000983"
     CDS             complement(30964..31374)
                     /gene="menI"
                     /locus_tag="OO544_000983"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HCR6766645.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            complement(31371..34427)
                     /locus_tag="OO544_000984"
     CDS             complement(31371..34427)
                     /locus_tag="OO544_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCR6766646.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKTNTTIGRIYNTVY
                     QRCRLQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITPLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESHPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            34816..35928
                     /gene="ydiK"
                     /locus_tag="OO544_000985"
     CDS             34816..35928
                     /gene="ydiK"
                     /locus_tag="OO544_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HCR6766647.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGSIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
BASE COUNT         8879 a         9432 c         8521 g         9192 t
ORIGIN      
        1 atgccggcaa actggcgtat ttataaccat ttttagcttt cactgctgca tttttttgcg
       61 ctcgccaacg aaacgtattt tttaacaata aaagctatta actttctctt cttctatgca
      121 ttagaatcat caagttttgt aaatcagacg caggcatgat agacctgcct taacagaggg
      181 acgctcagtg gcgcggataa accgtatttc gatcacgctc tgtgctttac tttttaccac
      241 cctgccttta acgcctatgg cccatgcttc aaagcaagcc agggagagtt ctgctaccac
      301 tcatatcacc aaaaaagcag ataaaaagaa aagcacggca accaccaaaa agacccagaa
      361 aacagcgaaa aaagccgcca gtaaaagtac gaccaaaagc aaaaccgctt cttccgttaa
      421 aaaatcttcc attaccgctt ctaaaaacgc caaaactcgc agcaaacacg ccgtcaataa
      481 aacggcctca gccagcttta ccgaaaagtg taccaaacgt aagggctata aatcgcattg
      541 tgtgaaagtc aaaaatgccg cgtcaggaac tcttgccgac gcgcacaaag cgaaggtgca
      601 aaaagctacg aaagtggcaa tgaataaact gatgcagcaa attggtaagc catatcgttg
      661 gggtggcagc tcaccgcgta ccggttttga ttgcagcggc ctggtttatt acgcttataa
      721 agatttggtg aaaattcgta ttccgcgtac ggcgaatgaa atgtatcacc tgcgtgatgc
      781 agcgccaatc gaacgtagtg aactgaaaaa cggcgacctg gtctttttcc gtactcaggg
      841 acgcggcaca gccgatcatg tcggcgtgta tgtcggcaac ggcaaattta ttcagtcacc
      901 gcgcacaggt caggaaattc aaatcacttc tctcagtgaa gactactggc agcgccacta
      961 tgttggcgct cgtcgggtaa tgaccccaaa aacacttcgc taaaacttta ccctgttgtt
     1021 acggcaacag ggtaagttca tcttttgtct caccttttaa tttgctaccc tatccatacg
     1081 cacaataagg ctattgtacg tatgcaaatt aataataaag gagagtagca atgtcattcg
     1141 aattacctgc actaccatat gctaaagatg ctctggcacc gcacatttct gcggaaacta
     1201 tcgagtatca ctacggcaag catcatcaga cttatgtcac taacctgaac aacctgatta
     1261 aaggtaccgc gtttgaaggt aaatcactgg aagagattat tcgcagctct gaaggtggcg
     1321 tattcaacaa cgcagctcag gtctggaacc atactttcta ctggaactgc ctggcaccga
     1381 acgccggtgg cgaaccgact ggaaaagtcg ctgaagctat cgccgcatct tttggcagct
     1441 ttgccgattt caaagcgcag tttactgatg cagcgatcaa aaactttggt tctggctgga
     1501 cctggctggt gaaaaacagc gatggcaaac tggctatcgt ttcaacctct aacgcgggta
     1561 ctccgctgac caccgatgcg actccgctgc tgaccgttga tgtctgggaa cacgcttatt
     1621 acatcgacta tcgcaatgca cgtcctggct atctggagca cttctgggcg ctggtgaact
     1681 gggaattcgt agcgaaaaat ctcgctgcat aataactgat ggcaaatgca gcattgcctg
     1741 atgcgcttcg cttaccatgc caacgcggat gaggcgttaa cagcgcattc ggcaagataa
     1801 atcgcacgtt gtcagcaact gtaacgcaga aggttatcct tctgcgtttt tgtttaatta
     1861 gctgttggca acgcaaactg tttcaggttg ttttctggct gacataaaca ccagcaataa
     1921 tgccagtccc gcgacaatcg cccccatcac cggcacaaag ctgtatccca gcccagcgga
     1981 aattaccgca ccaccagccg ctgctcccag cgcatttcca agattaaagg caccaatatt
     2041 gactgatgaa gacagacctg gcgcttcact ggcgacacgc atcacgcgca tctgtaacgg
     2101 cggtacgacc gcaaaggttg ctgcgcccca caccaccatg ctaatagctg cgccgaactc
     2161 attgcgggcc aggaacgggg ttgccagcat aatcaccatc agcagcaaca aaaagccttt
     2221 caacgtgccg ttaactgaac gatctgccag tttgccgccg agatagttac cgatagagaa
     2281 tccgacacca atcagcacca gcattgccgt gacgaacacc ggtgttgcgt gggtaatact
     2341 ttgcagtacc ggagagatat aggtgtagag agtaaacatt gcgccagctc ccagtaccgt
     2401 cgtcagcaat gcagacagca cctgcggacg cattaatacc gccagctctt ttttcacttc
     2461 aggtcgtgcc cctgtaccac ctttaggtaa tgagaagaac agacttacca ttgaaatcac
     2521 tcccagcccc gccgttgcca gaaatgacat ccgccagccg atggtttcac ccaaccaggt
     2581 cgccgccggc acgccaccga tatttgccag ggttaacccc ataaacatag tggcaactgc
     2641 gctggcctgt ttatgttttg gcaccacgct tgcggccacg actgaaccca aaccaaaaaa
     2701 tgctccgtga ttcaggctgg tcaaaatgcg tgaaagcatc agggtcatat aatccggcgc
     2761 gatggcggaa agtacgttgc cgagcgtgaa aattgccatc aggaaaatca acgcactgcg
     2821 gcgggcacga tgagaaagta gaagcgtcat cagcggcgcg cccaccatta cgccaactgc
     2881 ataggcactg attaacattc cggcagcggg aatcgagaca tccacaccgc gcgcaatgac
     2941 gggcaacaag cccattggcg agaactccgt tgtcccgata ccaaacgcgc caatcgccag
     3001 cgccagcaac ggatagttaa ttttcatgcc ttatctccac ctcttcgcgt cattacgcga
     3061 tattcattaa agtggcgaaa gcatgacagc aatcacaaaa aaatgaaaat aacaaaaaga
     3121 gaaaacactt ttgccatttt gctaacaaac aggaaggaga tgcgagggag aacgcgctcc
     3181 ctcgagagga aatcagtgca gcgcggcagt caaacccacg gctacgatca aaccgaggac
     3241 gataatcgtt gttaccagtg aaaatttaag gtcggtgctc atcaagtttt ctcctttttt
     3301 attaccacac aaaaagtgat attacgcatt tttacacact gtgatgaaaa aatctcccgt
     3361 catttataat gataagtgtt tttaccactt ccccttttcg tcaagatcgg ccaaaattcc
     3421 acgcttacac tatttgcgta ctggccattg accccttcct gacgctccgt gtcgtttttc
     3481 cggcgtaccg caacactttt gttgtgcgta aggtgtgtaa aggcaaacgt ttaccttgcg
     3541 attttgcagg agctgaagtt agggtctgga gtgaaatgga atggcaacaa taaaagatgt
     3601 agcgaaacga gcaaacgttt ccactacaac tgtgtcacac gtgatcaaca aaacacgttt
     3661 cgtcgctgaa gaaacgcgca acgccgtgtg ggcagcgatt aaagaattac actactcccc
     3721 tagcgcggtg gcgcgtagcc tgaaggttaa ccacaccaag tctatcggtt tgctggcgac
     3781 cagcagcgaa gcggcctatt ttgccgagat cattgaagca gttgaaaaaa attgcttcca
     3841 gaaaggttat accctgattc tgggcaatgc gtggaacaat cttgagaaac agcgggctta
     3901 tctgtcgatg atggcgcaaa aacgcgtcga tggtctgctg gtgatgtgtt ctgagtaccc
     3961 agagccgttg ctggcgatgc tggaagagta tcgccatatc ccaatggtgg tgatggactg
     4021 gggtgaagca aaagctgact tcaccgatgc ggtcattgat aacgcgttcg aaggcggcta
     4081 catggccggg cgttatctga ttgaacgcgg tcaccgcgaa atcggcgtta tccccggccc
     4141 gctggaacgt aacactggcg caggccgcct tgtcggtttt atgaaggcga tggaagaagc
     4201 aatgatcaag gtgccggaaa gctggattgt gcagggtgac tttgaacctg aatccggtta
     4261 tcgcgccatg cagcaaatcc tgtcgcagcc gcatcgccct actgccgtct tctgtggtgg
     4321 cgatatcatg gcaatgggcg cactttgtgc tgctgatgaa atggggctgc gcgtcccgca
     4381 ggatgtttcg ctaattggtt atgataacgt gcgcaacgcc cgctatttta cgccggcgct
     4441 gaccacaatc catcagccaa aagattcgct gggtgaaaca gcgttcaaca tgctgttgga
     4501 tcgtatcgtc aacaaacgtg aagaaccgca gtccattgaa gtgcatccgc gcttgattga
     4561 acgccgctcc gtggctgacg gcccgttccg cgactatcgt cgttaatcac ccgttgcggg
     4621 agtctcttcc ggctcccgca gccactcctt attcagcgtc tcactatcgc cgagatactc
     4681 aagcaaccag gttaacgcag gcgacatatc attttgctgc catgtcagac aacatgccga
     4741 atccggaaag gggttttcca gttcaagtgc tacccacttc ccctcattaa gccacggttt
     4801 ggcgaaatgt attggcacca tccctatgca taatcctgcc gagatacagg ttgccgatga
     4861 ttcccagtca ggcacgacaa ctcttttttg gttatccagc aaccaggtaa tacgtttagg
     4921 tagcgttcgc gaggtgtctt cgcgcaccaa cgacggccag ttgcgcaacg tatcatcgct
     4981 gaacgggcca tccatcaacg ccagcgggtg atggctggca acaacgcaac tccagcttag
     5041 catccccata tcccggaagg cataacgacc gcctaccgga atcgcgcgtg ttgcgccaat
     5101 cgccagttcc acgcgcccgt cggaaagcgc atcccagaca ccgttgaaca cttcctgaaa
     5161 gacaagaagt tcgacatcat caaaatggcg ataaaaatca acgatcatct gccgtgtacg
     5221 ttctggcctg acaatattat ccactgcgat agctaactga ccgcgccagc cgttcgctat
     5281 ctgctgacat tgctggcggg tgatctgcat ttttttgaca acagagcgcc cttctttgag
     5341 aaaccacgct ccagcagcgg tcagctccac atcacggtgc cgtcgttcaa agagcggcac
     5401 cgccagccac tcttccagct gacgcacggt atagctgacc gcagaaggaa cgcgatgcag
     5461 ctcctgtgcc gcagcgctaa aactaccatt acgcgctacc gcatcaacaa cttcgagtga
     5521 atattctgac cacatagtct gcctgcaaaa tttttgaaac caatcatcaa atattaccgt
     5581 ttcacaacac taatttcact ccctacactt tgcggcggtg tttaattgag agatttagag
     5641 aatatacatg caacctggga aaagattttt agtctggctg gcgggtttga gcgtactcgg
     5701 ttttctggca accgatatgt atctgcctgc tttcgccgcc atacaggccg acctgcaaac
     5761 gcctgcgtct gctgtcagtg ccagccttag tctgttcctt gccggttttg ccgcagccca
     5821 gcttctgtgg gggccgctct ccgaccgtta tggtcgtaaa ccggtattat taatcggtat
     5881 gacaattttt gcgttaggta gtctggggat gctgtgggta gaaaacgccg ctacgctgct
     5941 ggtattgcgt tttgtacagg ctgtgggtgt ctgcgccgcg gcggttatct ggcaagcgtt
     6001 agtgacggat tattatcctt cacagaaagt taaccgaatt tttgcgacca tcataccgct
     6061 ggtgggtcta tctccggccc tggctcctct gttaggaagc tggctgctgg tccatttttc
     6121 ctggcaggcg attttcgcca ccctgtttgc cattaccgtg gtgctgattc tgcctatttt
     6181 ctggctcaaa cccacgacga aggcccgtaa caatagtcag gatggtctga cctttaccga
     6241 cctgctacgt tctaaaacct atcgcggcaa cgtgctgata tacgcggcct gttcagccag
     6301 tttttttgca tggctgaccg gttcaccgtt catccttagt gaaatgggct acagcccggc
     6361 agttattggt ttaagttatg tcccgcaaac tatcgcgttt ctgattggtg gttatggctg
     6421 tcgcgccgcg ctgcagaaat ggcaaggcaa gcagttatta ccgtggttgc tggtgctgtt
     6481 tgctgtcagc gtcattgcga cctgggctgc gggcttcatt agccatgtgt cgctggtcga
     6541 aatcctgatc ccattctgtg tgatggcgat tgccaatggc gcgatctacc ctattgttgt
     6601 cgcccaggcg ctgcgtccct tcccacacgc aactggtcgc gccgcagcgt tgcagaacac
     6661 tctacaactg ggtctgtgct tcctcgcaag tctggtagtt tcctggctta ttagtatcag
     6721 cacgccattg ctcaccacca ccagcgtgat gttatcaaca gtagtgctgg tcgcgctggg
     6781 ttacatgatg caacgttgtg aagaagctgg ctgccagaat catggcaatg ccgaagtcgc
     6841 tcatagcgaa tcacactgat ctatatcgat atacttatac ttaggctgct aacaaaattt
     6901 tgttgtatga ttgaaattag cggcctatac taatttcgag ttgttaaagc tacgataaat
     6961 attatgtttt tacggggaca ggatcgttcc cgactcacta tggatggtca tttcggcaag
     7021 ggttcctcct ttccctctgt tctacgtcgg attatagact cgcggttttt tctgcgagat
     7081 ttctcacaaa gcccaaaaag cgtctacgct gttttaaggt tctgatcacc gaccagtgat
     7141 ggagaaacta tgagttcatc gtgtatagaa gaagtcagtg taccggatga caactggtac
     7201 cgtatcgcca acgaattact tagccgtgcc ggtatagcca ttaacggttc tgccccggcg
     7261 gatattcgtg tgaaaaaccc cgattttttt aaacgcgttc tgcaagaagg ctctttgggg
     7321 ttaggcgaaa gttatatgga tggctggtgg gaatgtgacc gactggatat gttttttagc
     7381 aaagtcttac gcgcaggtct cgagaaccaa ctcccccatc atttcaaaga cacgctgcgt
     7441 attgccggcg ctcgtctctt caatctgcag agtaaaaaac gtgcctggat agtcggcaag
     7501 gagcattacg atttgggtaa tgacttgttc agccgcatgc ttgatccctt catgcaatat
     7561 tcctgcgctt actggaaaga tgccgataat ctggaatctg cccagcaggc gaagctcaaa
     7621 atgatttgtg aaaaattgca gttaaaacct gggatgcgcg tactggatat tggctgcggc
     7681 tggggcggac tggcacacta catggcatct aattatgacg taagcgtggt gggcgtcacc
     7741 atttctgccg aacagcaaaa aatggctcag gaacgctgtg aaggcctgga tgtcaccatt
     7801 ttgctgcaag attatcgtga cctgaacgac cagtttgatc gtattgtttc tgtggggatg
     7861 ttcgagcacg tcggaccgaa aaattacgat acctattttg cggtggtgga tcgtaatttg
     7921 aaaccggaag gcatattcct gctccatact atcggttcga aaaaaaccga tctgaatgtt
     7981 gatccctgga ttaataaata tatttttccg aacggttgcc tgccctctgt acgccagatt
     8041 gctcagtcca gcgaacccca ctttgtgatg gaagactggc ataacttcgg tgctgattac
     8101 gatactacgt tgatggcgtg gtatgaacga ttcctcgccg catggccaga aattgcggat
     8161 aactacagtg aacgctttaa acggatgttt acctattatc tgaatgcctg cgcaggtgct
     8221 ttccgcgccc gtgatattca gctctggcag gtcgtgttct cacgcggtgt tgaaaacggc
     8281 cttcgagtcg ctcgctaaag gctattctat cgccccctct ccgggggcga tttcagatca
     8341 ggcttctgta cctggttgat tcatggcatt ttctcgtgcc gccagcacac gttctaccgt
     8401 atctaccact gcctgagttt gtggatcaat ttcaatgttg acgcgtgcgc cgagtttttt
     8461 cttcccaaga gtcgtgcgtt ccagagtttc cggaattaaa tggacgcaaa aacgcgttgg
     8521 cgtgacttcg ccgacggtca ggctaatacc gtcgatgcca ataaatcctt tgtacaggat
     8581 atatttcatc aactgactat cctggacttt aaaccagatc tggcgattat tttccgaggt
     8641 taatattttc gccacttcag cggtggtcat aatatgacct gacattaagt gtccgccaat
     8701 ttcatcgctg aattttgccg cacgctcaac gtttacccaa tcccccactt ttaaatcgcc
     8761 aagattggta atgcgtaacg tttctttcat caggtcaaaa ctgacatggt tgccgttaat
     8821 ttccgtcact gtcaggcagc aaccgttatg cgccacggaa gcacctgttt ccaggccgtc
     8881 cagcatgtgg tcgggtaact ccaccacatg cgtacgaaaa tttggtttct cgtcaatcga
     8941 caccagtttt gcggtgccct gtacaatccc cgtaaacata cttacaactc ctgaaatcag
     9001 ttaagacatt ctgttcagca caatagcagg tggaaaacgc ccttaccagt gaaggggtaa
     9061 gaatgactat tttttcactg gagaattaat aaatcctcgc tacaatagac tgaatttccc
     9121 ctgcttcttc tttttgctgc ccattcaggc ggctttttag tctctcatat aactacaaat
     9181 aaaaggtgtt cacgtgcaga agtatatcag tgaagcgcgt ctgttattag cattagcaat
     9241 cccggtgatt ctcgcgcaaa tcgcccaaac tgcgatgggt tttgtcgata ccgtgatggc
     9301 gggcggctat agtgccaccg acatggcggc ggtcgctatc ggtacttcta tctggcttcc
     9361 ggcgatcctc tttggtcacg gactgctgct ggcattaacg ccggttatcg cgcaattaaa
     9421 tggttccggt cgacgtgagc gcattgcgca tcaggtgcga caaggtttct ggctggcagg
     9481 ttttgtttcc gttctcatta tgctggtgct gtggaatgca ggttacatta tccgctccat
     9541 ggaaaacatc gatccggctc tggcggacaa agccgtgggt tatctgcgtg cgttgttgtg
     9601 gggcgcgcca ggctatcttt tcttccaggt tgcccgtaac cagtgtgaag gtctggcaaa
     9661 aaccaagccg ggtatggtaa tgggctttat cggcctgctg gtgaacattc cggtgaacta
     9721 tatctttatt tatggtcatt tcggtatgcc tgagctcggt ggcgttggtt gtggcgtggc
     9781 tactgcggcg gtgtattggg ttatgttcct tgccatggtt tcttacatta aacgcgcccg
     9841 ctccatgcgc gatatccgta acgaaaaagg caccgcaaaa cccgatcctg cggttatgaa
     9901 acgactgatt caactcggtt tgccgattgc gctggcacta ttctttgaag tgacactgtt
     9961 tgccgtcgtg gctctgttag tgtctccgct cggtattgtt gatgtcgcag gacaccagat
    10021 tgccctgaac tttagttcac taatgttcgt gcttccaatg tcgctggcgg cagcggtaac
    10081 tatccgcgta ggttatcgtc tgggtcaggg ctcaacgctg gatgcgcaaa ccgctgctcg
    10141 gaccgggctt atggtgggtg tctgtatggc aaccctgacg gccattttca cggtttcact
    10201 gcgggagcaa atcgccctgt tgtacaacga caatcccgag gttgtaacgc tggctgcgca
    10261 tttgatgttg ctggcggcgg tatatcagat ttctgactca atccaggtga ttggcagtgg
    10321 gattttgcgt ggttataaag atacgcgttc cattttctat atcaccttta cagcttactg
    10381 ggtgctgggc ttgccaagcg gctatattct ggcactgacc gatctggtcg ttgaacctat
    10441 ggggccagca ggcttctgga taggctttat tatcggcctg acatcggcag ccattatgat
    10501 gatgctgcgt atgcggttcc tgcaacgtat gccgtcagcc atcattctgc aacgagcgtc
    10561 ccgctaataa agacaaggcg caaccttcac gggttgcgcc tgtgttttta cgcaggctgg
    10621 agcgttgcgc caatcccgtc ttcgtctggc tgtaatttca gagcgttaca cagagtttgc
    10681 cgactggcat ctgttatctc aacgcgtata tccccgttaa cttccccttt cacattgatg
    10741 aagtcaggtg aaatttcttt cgcactggtt tgcaggcaaa tcgctccctc tccgcgcagg
    10801 cgtttcactt tcagttgcac atgcgttgcc gcaccagtga tcagccacag ttttccgcca
    10861 ttcagattga tgttaccaac aatgaaagtg acgctgtcac cttttaccgt actgccgtcg
    10921 agaatcaaca caccgccctg ctgtacagta atcgttgatt gttgcagctc tgcgccataa
    10981 ccaataatca ttgaagcgcc gtccagtaat gcaacactcc cggtcagttc aaccggtgcg
    11041 cgatcttcct ctacgacaaa attttcgtcc gcgctggtgg gtgacaacag gatcactgtt
    11101 ccaccttgta ttaccgcatt ttccagcgta ccggcatgca cttcagcccg cccccccaca
    11161 ttcaccgaca ggttttgcgt cttaccggaa ctgtagagct gaaggccatc cgtccccaga
    11221 cgataaatgg atccattctc aacaatagtg tcagagtctt caccaccgag attattcatg
    11281 gtgccaccag caagcaacgt atcactggct ttcccggcca gcataaaaac gccgccctga
    11341 ttaatctcac aattgctggc ttctccactt ggatacacca acaattcacc accttcatct
    11401 accacgaccg ctgaggttgt cccattaacc aacaggccgc cctcttgatc gagaatgatt
    11461 ttttccgcgc gatgtccttc cagtacacgc aggttaccgc cattttccag caacaaaccg
    11521 caggcataac cctgatcaac gctgaattca ccttcgggat ggcggccatt aaccgtagcg
    11581 agcgtagaga gactaattgc gcctccctcg gactgaacaa tatctgtggc aatggcaccg
    11641 tcgctgaccg atagcgtgcc attgttaccg tactgtgtgc atttggcgat cccaccggtt
    11701 gccactttca accagccagc ccccgtgacc acggtatttt cggctcttcc gccagcttca
    11761 acaatcaact cgccttcagt aagttgcgtc gcgccaatga cctcgccggt cttaacaatt
    11821 tgcacattcc cgtcgctcat caagtttttc gcatcagatc ccatgattta ttcctttgct
    11881 gcatctgtgt gcctttattg ctacctaagt gtaaaggcta cggaggattt atccacgaca
    11941 gatttgagat ggtggcaaac aattctgttt aaactctgat acacgaatta ttgggttgta
    12001 tcagatgtaa atgcgatcct gaataaaaat cgcccttgca aatcaacaaa atatagccaa
    12061 tttgcttaaa agctctgcaa acggtcgaaa tagcgcagaa aattacgttt tgcctcttgc
    12121 cacctttcca ctctgccgct aatatttgtc cccgttgtca cctacaacgt tgcgttcata
    12181 gctcagttgg ttagagcacc accttgacat ggtgggggtc gttggttcga gtccaattga
    12241 acgcaccatc ctgcgtccgt agctcagttg gttagagcac caccttgaca tggtgggggt
    12301 cggtggttcg agtccactcg gacgcaccag attttcttaa tctggtcttt tcctttttcc
    12361 ctctgtttat tctctgtatc caatacgtta aaagatttac actgtcttcg tatgcgttat
    12421 cagaaggaga atcgctatgg caactttgtt acaacttcat tttgctttta atggcccatt
    12481 tggcgacgca atggctgagc agcttaaacc acttgctgag tcgattaatc aggaacctgg
    12541 ttttctgtgg aaggtatgga cagaaagtga aaagaaccac gaagccggtg ggatctacct
    12601 tttcactgat gaaaaaagcg cccttgccta tctggaaaaa catactgccc gactgaaaaa
    12661 tctcggcgtt gaggaagttg tcgccaaagt tttcgatgtc aatgagccac ttagtcaaat
    12721 caatcaggca aaactcgcct gacagaattt aatcaagggc ggttagcgcc cttttcatcc
    12781 ctgtctgaaa tttctcaaat tctaaaaatc tcaaccaaac ttatctgata acactaaatt
    12841 cgaaagaatg cgtacaggta agtaacaatg aaaaaaattg ctattgtggg tgccgggcct
    12901 acggggatct acaccttatt ctcgcttcta cagcaacaaa ctccactttc tatttctatc
    12961 ttcgagcagg ctgacgaggc cggtgtcggg atgccataca gtgatgagga aaactcaaaa
    13021 atgatgctgg caaatattgc cagtattgaa ataccgccga ttaattgtac gtatctcgaa
    13081 tggctacaaa agcaagaagc cagccatctc cagcgttatg gcgttaaaaa agaaaccttg
    13141 cacgatcgtc agtttttacc gcgaattctg ctgggcgaat atttccgcga tcaattttta
    13201 cgattagtag accaggcacg acagcaaaaa tttgcagtgg ctgtttatga atcatgccag
    13261 gttaccgatc tgcaaattac aaatgctggc gtcatgctcg ctacaaatca ggatttaccc
    13321 agcgagacgt ttgatttagc ggtgattgcc acgggtcacg tctggcctga tgaagaagaa
    13381 gcaaccagaa cgtattttcc cagcccgtgg tcaggcctga tggaagcaaa ggtcgatgcg
    13441 tgtaacgtgg gtattatggg aacatccttg agcggactgg atgcggcaat ggcagtggct
    13501 attcagcatg gttcgttcat tgaagatgat aaacaacacg tcgtttttca ccgcgataac
    13561 gcaagtgaaa agctaaatat cacgttgatg tcgcgcacgg gtattttacc cgaagccgat
    13621 ttctattgcc ctattcccta cgagccctta cacatcgtca ccgatcaggc attaaatgct
    13681 gagattcaaa aaggcgaata tggccttttg gatcgggtat ttagattgat agtagaggaa
    13741 atcaagtttg ctgatccaga ctggagccaa cgcatagcct tagagagcct gaatgtcgat
    13801 tcctttgctc aagcctggtt tgccgagcgc aaacaacgcg acccatttga ctgggcagaa
    13861 aaaaatctcc aggaagtcga acgcaataaa cgagaaaaac atactgttcc ctggcgttat
    13921 gtcattctgc gcctgcatga agccgtacag gaaattgttc cacatctgaa tgaacacgac
    13981 cataaacggt tcagtaaagg ccttgcccgg gttttcatcg ataattatgc ggcaatccct
    14041 tcagagtcta ttcgtcggct gctggcctta cgtgaagcgg ggatcattca tattctcgcc
    14101 ctcggtgaag actacaaaat ggaaattaat gagtcgcgca ccgtcctgaa aacggaagac
    14161 aacagctact cgtttgacgt ttttattgat gcccgcggac agcgtccgct taaagtgaaa
    14221 gatattcctt tccctgggct acgcgagcaa ttacagaaaa caggggatga aatccctgat
    14281 gttggcgaag attatacgtt acagcaaccc gaagatattc gtggacgcgt agcgttcggc
    14341 gcgttgccct ggttgatgca cgaccagcct ttcgttcagg gacttacggc atgtgcagaa
    14401 attggtgagg cgatggctcg ggcggtcgta aagcctgcat cccgtgctcg tcggcgtctt
    14461 tcgtttgatt aaaggtaaag ctatcagcac tcaacaaact gatgctgttc attaagacga
    14521 tagcggactc cggttcgaat attgttttca ccttcaatgg cgacggcgaa gcgaactctt
    14581 tcgccgtcat gatatgccag tgcggcgctt ccccctggcc cgagaataat tgagtcgacc
    14641 acgcccgtgc tcactaccgt tgaattttca ccgctggcaa tgatgtgtac attgtcgccg
    14701 ctgttggcaa ttctggcgtt tgcgccaaag ctggcgattt gcaccaggtc accattactg
    14761 gcgacatgac accgttcacc caatgtacaa acgcgtactc gcattccggt attcgcaatc
    14821 cgactactat ctccagcgct gcttatccgc gaagaattac cggttacagc aacccgtgcg
    14881 cgttcaccgg agctgccaat gtgactgtta aagccgacac ttccaatccg cacgctataa
    14941 cccatactgg caatttgcgc ggcataccca gcggttacga ttcgggcgtt atcctcacaa
    15001 cagccgatgc gagcatgatc gtcgctgcgg gcaaactgac tgcaaaatgg tatctctccg
    15061 gggtctgtgg ccagtttttg catagaggaa acttcctgat gcgcgaagtt ttcatccgcc
    15121 agccattttt tccacgcata ctcaaccaga ctctccgccc agtccgtata tccttcctca
    15181 atcagtgcat gatgaatatc ggcataacta ccgccgcgtg ggaaatggcg caggaaccag
    15241 cgatacatca ccgctccgat acgccattct cgcaggtctg ctcgtgtaat gatcattcgt
    15301 tagtgccggt gatagccatc gaccatgctt ttaaaggttt cgtgtggcgt acgccccgtg
    15361 gtgcaaagat aaagatgtcc gaagataaaa aagaggctta taaatgccag agcaaaatgc
    15421 gcctgcaata accagtatct tacgccagga aacacatctc ccacggcttg cggatagaga
    15481 cacaacagcc ccgtcaatag taacaacggc agcaatccac acatgacacc aacataggcg
    15541 acctgctgta agggattaaa tttagactgt gttgttgccg ggaaaggatg ttcttccccc
    15601 tgcataatgc caaacaaata aaatcgtgtt tgttttgccg ctcgttccag ccacccctga
    15661 cgacgaatgc gatagtggtg accattatcc ccaacggcat tgatcagcac aaagccgagc
    15721 cagcatgcca gtaacaaaaa tccgcaaact tcatgcaccg caaccagact tttaaccgca
    15781 gttgcgccca ccatcgcaaa gtgatttatc agcccacttg ccagcaacaa tacaaagagt
    15841 aacgcattcg accagtgcca taatctgacc gctttgctgt aaagataaac cttctcacca
    15901 tgatcggtgg ctgatttctt cacgccacga gcacgaagca aggcgtgcag ccccaacacc
    15961 aaccacatcc caaccagcaa tactccggca atgatcaacc acaccggcca aaattcggga
    16021 gtgaactgtg gcacatagtt cgctaactgg ctctgaaact gttcagcatg ttgcgacggg
    16081 ttcatacatt ttccttttta atcaaatgtt gaccgaaccg gcgatacaga tgcggtttcc
    16141 cggcaccaag tagctgatat tgatagtatt tattttcctg caaccacgcc tgaatttctg
    16201 ggctatcttc gcgaccaaag atcaatgcgt gttccggaca ggcgctgacg caaatgggcg
    16261 gaaagccttt tgccagtcgg gactcagcgc agaaatcaca tttatctgcc actttagtga
    16321 cagggttaag gtagcgaacc tggtacggac aagcgccaat gcaataactg cagccgatgc
    16381 actgcgattt ttctacccgc acgatcccct gctcatcacg ccacgacgcc cctgtcgggc
    16441 atacgtcgat gcacggagca tcttcgcagt gctgacatga ctggcgaaaa aagtgatact
    16501 gcgtttcgtt gtcgttatcg gtgactggaa tgtgtgctat cgataaacgg cttccctgag
    16561 cggggacgtg gttagtttta cggcatgcgc gggcgcaaat attacagcca ttgcaccgtg
    16621 actcgtcatg aatcatggca taccgaactt ccttttcttg cctcgtgttc gctaacagag
    16681 aagacgcaga accagtaaaa aagattactg ttcccatccc cagaacaaat ttgcgtcgag
    16741 tgaaggacat ctgattatcc cctaatgcag tggctgcaac tgtccaccct atccatgaca
    16801 tctccctgaa atttcagggg ttctgaaact gctctacctc gaaccattcg cataagccat
    16861 aagccttgcc ataggtcata aatcgctcgc taaacaagta caacaccgcc gtcgatgact
    16921 ccacctgccg tatatcggca tattcaggtg caacatccat cgcggcgatc gcggcttcaa
    16981 tctgcgcttc ctggaagtaa tatggcgctt gcatcagcat ggcgactttg taaggacgag
    17041 gatacgtctg gcactcaaaa cgaacggcgt gtgcaatggc gcgacaaatg tcctgctcca
    17101 caacttgcaa agacatcgcc gcgtagtttt cactcatggc ctgcgttgag taatagtaat
    17161 catcctgact tcccgtcata caagcgatat ccgcgtagtc tccgttctgc ttaatgcttt
    17221 ccagcaaaac tgacagttcc tcttctgcaa ccgaataggg tggcgagaga aacacggtgc
    17281 gcgccaccag ctgcccagcc gcagaatgct ggcgaatgaa ctcagccagg atttcagcag
    17341 gttccggttc ggttaccgta tcaacggggt gacgcatccc ctgcaaccat tgccgcttcg
    17401 cctcgtcaac ttcgctgact ttcgctaacg gcaattcatc ctgatggttc atcttaccca
    17461 ttccttacgc aggcagtaga ttgtgtgctg ccagatcggc ggcaatatct tccagcccca
    17521 gccgctgcaa tgtttcgcgg gttggacaac caagctgtgg gtcccagccc atttctttgt
    17581 agaacatcgt gagcgaagca tgcatatcgt cacgatccat tttgtcagta ccttcagtaa
    17641 agaccgggat ctgcggatcc ttgtcgaaaa cccaggaaca gataagatcg tgttcattac
    17701 gcatatcttt ggtttgcatc agttttaccg tgtaggcacg atgcaacgta aagatacgct
    17761 ctgcagctaa atctaatttt tcctgagtca tctcttcgcc ggtgatcgct ttgaagaatt
    17821 tggcttcaag cgccagatcg ccccggtaat tacggctttt cagcggcgaa acggtcattg
    17881 gccagaccca gttgcacagg gtgacggcgt tatgcaaaca gacccgcaac agcgaccact
    17941 ttgcatattt aatttttgcg tcattgattg gcgtgtagtt tttggtttca tcgtaagcat
    18001 cttcagaacc aaaaagttct ttcgccactt cacgttgcag tttcaatggc aagccggaac
    18061 caataaagtt gatatgggta tgcgtcatgc agtcacggtt gaacatacag ttaacaatgg
    18121 aaccgacctg cgctgacgct tcattggcat ggtgaaccgg atagccaaac ggcgaccaga
    18181 gtttattttt cgcgtagccc cagtactctt cacccaaatt ccagcgttct gcgatggcat
    18241 atgaaccatc agccaggtga ctcagctcac ccacacgatg cgccagacgg tagtaaaaat
    18301 ctttaatttt aatgaagtta acgtcacccg cttccagttg atcccagcga atttccgcat
    18361 actcttcagc aggcagaaca cgcttgaaca cacctttgct gtaacaatag gtaaagtcgc
    18421 gatgcaactg cccgtagtta caccataggc cgtagtcatc gaacagattc agacccacca
    18481 ggttaccaat cacacgacca tcgtctttat cttcaaaatc tttcggaccg ttcgggaaga
    18541 tggtggtatg gacaaagttg gctacacaag tgttaccacc tgtgctgggc acgccaaact
    18601 ctttcacccg aggaatattc atttgggtca tacaacggat cgggcaagag tggcagccac
    18661 tcatttttac tgtgtatttc tctgccgccg gacctaagtc aaaaacggat ttataggtac
    18721 gaaagccgac cgtgttctga ttgcctggcg gaatttcccc cgtttcaatc ggaccacctt
    18781 cagccgcgcc ccaaaacagc cctttacgtg ctgtccaacg tgacttggga tctgaatact
    18841 ctgcccacga ttgcggcgta cttggcacga catggttgtt attcgcacca ataagttcag
    18901 tcatcatgta atcgttgaga cgcttcatct cctgacgatc ggcaatgttg acccctttcg
    18961 tcccttcaac cgcaatcgct ttcaggtttt tcgaacccat tattgcgcca gttcccgcac
    19021 cgccgctgtg gttacggcta ttcaacatgc cagagagagg aacaaggttt tccccagcct
    19081 gaccaatagc cgccacacag gtttccggac tggtcaatcg acaaatttct tccgtcgttg
    19141 cgcgcgtccc ttttccccat aagaaatcgg ctttttccag gctaactttg tcatctttaa
    19201 tcttcagcca taccggtgat ttcgccttcc cttcgataat aatgacgtcg tatccagcga
    19261 atttcatttg cgctgcaaaa aagccaccca tatgggcatc gacgactaaa tttcctttgg
    19321 taaaagtaga aagtgaggtg atatttacgc gagaactaca gggggcacca gatccagtta
    19381 atgggccggt agcaaagact aatttattcg cttcatcgaa aggtttcgtg cctggcggta
    19441 cttcgtcata cataattttg tagccgaagc ccatgccacc gacaaaactt ttaaacttac
    19501 tggaatcttc gagggtaata tttcctgtcg tgagattgac tcttaatata ttacctgtcc
    19561 aaccgttagc catgattttt tcctttgcaa gattcacaca gtaatatctt tccactcgat
    19621 aatttttaac gcccctgttg ggcaggcgtt tgcgcattca ccgcataaca cacatttcga
    19681 ggatttttta ctttcggtat ttacggtggc catcatccac gggcatgccg tagtacaggc
    19741 gctacagcca atacagcgtt tatgatcgac ggtaatacag ccttctttct gctgccaggt
    19801 aatcgcacca atcgggcaga cgttcatgca ttgcggctct ttgcattgac ggcaggtgtc
    19861 cgcggtatag ttgagatcgc catacaggcc gccgccagag ccaaccccgt tgtcgccaaa
    19921 gaaataattg cgatggattt tgatacggga gaagaatgtc cctactgagc catcgttgaa
    19981 gttggtacaa gagatttcac agcggtggca acccgtgcat cgcgctcgct gggtcaccag
    20041 cacccctttg ggtgtattta tcaaccctac tgtgccgcta tcaatatctt cctgtttgca
    20101 acccaaaagt gacagtaacg caggggcaat tgttagacca gcaaggcctt ttcctgatat
    20161 acgcaagaat tctaatcgcg ttaagccaat atctaatagt ggacgatcaa ccgggttcat
    20221 ttatttcgca tcctcttcgc aaacacagtg ctgacaattc agcagcctga tattaagcat
    20281 tcaactaata cgctgctgac tctgtcccca acaccagata ttttccctaa cccgatagtg
    20341 tatgagaaaa gatttaacgc cgataaacca atcctttatg gttaggagaa aaaataatgt
    20401 gattattctg agcccttaac attgatcgtt atcaattaaa attacaaagc aagaaattat
    20461 aagtgaactg atatttatta tcaattgaaa taaatttaac ttaagcgaca attatttgtg
    20521 acgttcattg cagaaaataa aaataatatt atcgttatag attaaaatac ataccgcaat
    20581 tcgtataatt tcagcagttg ttatttagaa tgattaactg gaccgcaact gaagcaagaa
    20641 tatcaggatg aagcttaccg cctcatcctg aatggtcatt ttaccagtgc gttaatgacg
    20701 gtttccatcg cctgcttttc cagctcactg cgctgaatgc gtttattggc taatgcagta
    20761 cgtaatacgc ctgcgataac aatatgcttt ggttcctgcc ccagatcgcg catttccagc
    20821 acaactttac cgacaactct gcacatttct cggtacagtt catcctcttt tgtccgattt
    20881 cccattcttt atacctattt atcatgccaa ctatcagcat atatcaatct aaccaattaa
    20941 acaaaaatca aacaaaatca gacaaataac gcgataaatt attttaactg tagcaattga
    21001 gcgatgatat atttatacac cggatgaact ttcacttatc ctcacactga caacttcggc
    21061 accagacgtt gcgcaaacag cgaagttttt gcgtaacctt ttccctggaa cgttaaatct
    21121 ttgataacaa tttattgtct aacaagttgt atattttttg aaacgctgtt tttgttttcc
    21181 ttttggatta atttcagcgt ataatgcgcg ccaattgact cttgaatggt ttcagcactt
    21241 tggactgtag aactcaacga ctcaaaaaca ggcactcacg ttgggctgag acacaagcac
    21301 acattcctct gcacgctttt tcgatgtcac ctatccttag agcaaggcac caccactttc
    21361 gtaataccgg attcgctttc cggcagtgcg cccagaaagc aagtttctcc catccttctc
    21421 aacttaaaga ctaagactgt catgaaaaag accaaaattg tttgcaccat cggaccgaaa
    21481 accgaatctg aagagatgtt agctaaaatg ctggacgctg gcatgaacgt tatgcgtctg
    21541 aacttctctc atggtgacta tgcagaacac ggtcagcgca ttcagaatct gcgcaacgtg
    21601 atgagcaaaa ctggtaaaac cgccgctatc ctgcttgata ccaaaggtcc ggaaatccgc
    21661 accatgaaac tggaaggcgg taacgacgtt tctctgaaag cgggtcagac ctttactttc
    21721 accactgata aatctgttat cggcaacagc gaaatggttg cggtaacgta tgaaggtttc
    21781 actactgacc tgtctgttgg caacaccgta ctggttgacg atggtctgat cggtatggaa
    21841 gttactgcca ttgaaggtaa caaagttatc tgtaaagtgc tgaacaacgg cgacctgggc
    21901 gaaaacaaag gtgtgaacct gcctggcgtt tccattgccc tgccagcact ggctgaaaaa
    21961 gacaaacagg acctgatctt tggttgcgaa caaggcgtag actttgttgc tgcttccttt
    22021 attcgtaagc gttctgacgt tatcgaaatc cgtgagcacc tgaaagcgca cggcggcgaa
    22081 aacatccaca tcatctccaa aatcgaaaac caggaaggcc tcaacaactt cgacgaaatc
    22141 ctcgaagcct ctgacggcat catggttgcg cgtggcgacc tgggtgtaga aattccggta
    22201 gaagaagtta tcttcgccca gaagatgatg atcgaaaaat gtatccgtgc acgtaaagtc
    22261 gttatcactg cgacccagat gctggattcc atgatcaaaa acccacgccc gactcgcgca
    22321 gaagccggtg acgttgcaaa cgccatcctc gacggtactg acgcagtgat gctgtctggt
    22381 gaatccgcaa aaggtaaata cccgctggaa gcggtttcta tcatggcgac catctgcgaa
    22441 cgtaccgacc gcgtgatgaa cagccgtctc gagttcaaca atgacaaccg taaactgcgc
    22501 attaccgaag cggtatgccg tggtgccgtt gaaactgctg aaaaactgga tgctccgctg
    22561 atcgtggttg ctacccaggg cggtaaatct gctcgcgcag tacgtaaata cttcccggat
    22621 gccaccatac tggcactgac caccaacgaa aaaacggctc atcagttggt actgagcaaa
    22681 ggcgttgtgc cgcagctggt taaagagatc acttctactg atgatttcta ccgtctgggt
    22741 aaagaactgg ctctgcagag cggtctggca cacaaaggtg acgtcgtagt tatggtttct
    22801 ggtgcactgg taccgagcgg cactactaac accgcatctg ttcacgtcct gtaatattgc
    22861 ttttgtgaat taatttgtat atcgaagcgc cctgatgggc gcttttttat ttaatcgata
    22921 accagaagca ataaaaaatc aaatcggatt tcactatata atctcacttt atctaagatg
    22981 aatccgatgg aagcatcctg ttttctctca gtttttttat ctaaaaccca gcgttcgatg
    23041 cttctttgag cgaacgatca aaaataagtg ccttcccatc aaaaaaatat tctcaacata
    23101 aaaaactttg tgtaatactt gtaacgctac atggagatta actcaatcta gagggtatta
    23161 ataatgaaag ctactaaact ggtactgggc gcggtaatcc tgggttctac tctgctggca
    23221 ggttgctcca gcaacgctaa aatcgatcag ctgtcttctg acgttcagac tctgaacgct
    23281 aaagttgacc agctgagcaa cgacgtgaac gcaatgcgtt ccgacgttca ggctgctaaa
    23341 gatgacgcag ctcgtgctaa ccagcgtctg gacaacatgg ctactaaata ccgcaagtaa
    23401 tagtacctgt gaagtgaaaa atggcgcaca ttgtgcgcca tttttttgtc tgctatttac
    23461 cactactgcg tcgcgcgtaa catattccct tgctctggtt caccattctg cgctgactct
    23521 actgaaggcg cattgctggc tgcgggagtt gctccactgc tcaccgaaac cggataccct
    23581 gcccgacgat acaacgcttt atcgactaac ttctgatcta cagccttatt gtctttaaat
    23641 tgcgtaaagc ctgctggcag tgtgtatggc attgtctgaa cgttttgctg ttcttctgct
    23701 gatagtggtc gatgtacttc aacataacgc atcccgttag gttccacgga atatttcacc
    23761 ggttcgttga tcactttcac cggcgttccc gtccgcacgc tggagaacaa ggctttaata
    23821 tccggtgcat tcatgcgaat acaccctgaa ctgacgcgca aaccaacgct gtccggcgca
    23881 ctggtgccat gaatgaggta ttcgccatta ccatgcgcga ggcgcagtgc gtaacgtcct
    23941 agcgggttat ttggtccggc aggaacgact ggcggtaatt taatgccacg ctccagcgaa
    24001 cgctgacgaa tgcctgccgt aggcgtccag gttgggttag ggattttctg cccaacacgc
    24061 gtttccatca ccggcgtttc cagcccctgc aatccaatac ctattggata aacctgcaca
    24121 atattttctc ccggcggata ataataaagg cgcagctctg caaggttaac gataatcccc
    24181 tgacgcggtg catcaggtaa taacagttgt gaaggaatag ttatcgtcgt accaggtttt
    24241 ggcaccggga cgatagtgtt attggcttca aggatcaaca ttgccgcagt atcaaaacgt
    24301 cgggcaatag cctgaaggtt tttatcccct tcttgcaccg tatacgtttg attttgccca
    24361 accagtcggc ttccggttgg tggtagcgga taatcaaccg cccaggcagc ctggatggcg
    24421 ctaaaagcgc cgataagcgt gagtgtaagc aaagacgcgc gtttcattgt aaacctcctg
    24481 tatttgccgg agactcacgc tgaaacgtcg gatggcgctt atgttcacct gaaaccaaaa
    24541 cactcctgtg caggtcagtg taaacattga ccatccggca atgtgagcca accggatgaa
    24601 agctgtccct ttagtttagc taagtgcagc ggctttggcg cgaattgcgc gaatcatcgc
    24661 ttccagacct tgtgaacgag atggggtgag atgttgggtg agtgccattt tttcaaacca
    24721 cggacgcaca tcgaaactga caatatcctg cggcgtcatc tgatcgtaga gaataaagac
    24781 taccgcaata agccctttca caatcgccgc atcgctgtcg ccctgcaatt caataattcc
    24841 gtgcgcattc tggcgcatga caatccacac ctgactctgg cagccctgaa tgctattttg
    24901 tgggcttcgg tcttcgtcgc gtaattcagt cagacgctgg cccagctcaa taatgtagag
    24961 atatttctct tcccagttgg cgcagcgtaa aaaattacgc agcaattttt ctttatccgg
    25021 caataaagcc atagtgcctc cctgttatcc cagcaaacgg tgaatacgtt gcaggccggt
    25081 caccagacga tccacttctt catgggtgtt atacatggcc agcgacgccc gacacatcgc
    25141 agggacgttg taataggcca ttaatggcat agcgcagtga tgtccggtac gcacagcaat
    25201 gccgtaatta tcgagaaaac tgccaacatc ataggcgtgg tgtttaccga gattaaaagc
    25261 aataacgcca agcctgtttt gtgggccata gagagtgaga tccggtacag attccagctg
    25321 tgatagcgca taatgcatca gattctgttc atactcggct atgttattaa gccccagcgc
    25381 cgaaacatac tccagcgccg cgccaagacc aataatgccc ccggtattgg gtgtaccggc
    25441 ttcaaaccgc catggtgctt tggtccaggt agtgccttca ctcaggctga cggtggcgat
    25501 catagaaccg cccccttccc acggcggcat ctcctgcaac aaggcttctt tcacataaag
    25561 aatgccaatt ccggtggggc catacagttt atgcccggag aacacgtaaa agtcgcaatc
    25621 cagcgcctga acatccaccg gatgatgcat caccgcctga gcgccatcca ccagcacttt
    25681 tgcgccatgc tggtgcgcaa gcgtgattat ttccgccagc ggattttctg ttcccagcac
    25741 gttggagaca tgagtaattg ccagcaggcg agttttctca tcaaacagcg taggcagcgt
    25801 ctccagttgc agcgtaccgt cggggttgag agggatcaca cgcagctctg cgccaacgcg
    25861 tgcgcaaagc atctgccagg gaacaatgtt agcgtggtgc tccatctgac tgatgatgat
    25921 gttatcgccc gcccgcacgt tgctgttgcc ccaactattg gcgaccagat tgatcccttc
    25981 cgtcgtgccg cggacgaaca ccagctcttc cgccgaacgg gcattaataa acagcgatgc
    26041 ccgcttgcgc acgttctcca ttttctcggt cgcctgggcg cttaaggtat gaataccgcg
    26101 atgcaccgcc gcgtagccgt gacgataaaa ctcagcctcg gcgtcaatca cctggcttgg
    26161 tttctgcgca ctggcggcgc tatcgagata agccagcggc aaaccgttca cctcacgaga
    26221 aagcaccgga aagtcggccc gcactttgtc gacggaaaaa gtcatcttgc acctcctggc
    26281 agccgttgac cgattcgggc cagcacctgc tgtttaagcc cctcatcacg cagtgcttcc
    26341 gtcagttcgg cagcaaaggc gtaaatgatc atctgctggg catcctgctg attgatcccg
    26401 cgcgagcgca gatagaacat ctgttcatca tcaatacgcc ccaccgtcgc gccgtggctg
    26461 catttcacat catctgcata gatttccagc tgcggttttg tatccacttc cgccattttg
    26521 cccatcagca gattattgtt ggtcatctga ccatccgttt tgatggcgtg ctgcgcgacg
    26581 ttgatcaaac cgttaaatac cgcgcggcct ttgtcgctga cgatagtttt gtgcaactgt
    26641 cggctgttac aaaaaccttt attgtgttcc agccaggtac gggtatcaca cacctcgttt
    26701 ttcaccggca tcgccaggct attgatccgc agcgtgctgt tttcgccatt gagttgcgta
    26761 ctggtgttgt gtcgtaacac tgcgccaccc agcaggaaac tatggctaaa tgcggtggca
    26821 tcctcagcca gcaacaaatc gttatgagca aagtggtgac tgagcgggtt ttcaaacgcc
    26881 agcttgatat gctgcaagtg ggcgttcgct gcgacattga tagtgaaccg tgccccggta
    26941 aaatgacgag catcattaag gctgacaaaa tgttcgatca ccgttgcttc ggcaccttcc
    27001 gccagatcca gatgatgccg gtaatgggca gtgttcacct cttcacctgc cacgccctgg
    27061 gtgatatgca ttaacagcaa tggctttgcc ggccgttgac cgcgcttcac ggcgatatgc
    27121 gtcacgcttt gtgccaggct ttccgtcaaa tgcagaaaca cttccgcctg aatagcgtcg
    27181 ggtaacccct gacggtcgtc gttaatgctc acttcatatc cgctgccttc aattgcatcg
    27241 ctcagcgcgg gcacgtaacg tccatcgaca aacaccagcc gcacggcgtc taacgttaac
    27301 gctaaggcat cacgctgctg tggggatata tctcccgcaa tgctgacaaa ctgactattg
    27361 gtcagccctt ccagcggcgt atatttccag ttttcatgtt tacgtgtcgg cagtccggta
    27421 cgcagcaatt gttgtaaatg ctgctgtgct tgcggtgagc gttttgtccc ttcagcttca
    27481 aacaagtgat gccactgttg cagcgcatta ctgctgttcg gtaagccagc cataaccctg
    27541 ctcctccagt tgtttgacca acgtgaaatc gccggatttc acaattcgtc cctgatatag
    27601 cacatggacg taatcaggct tgatgtagtc gagaatgcgt tggtagtgcg taacaatgat
    27661 gaatgagcgc ttgccgtcac gcagcgagtt cacgccatcg gcaaccactt ttaatgcgtc
    27721 aatatccagc ccggagtccg attcatcaag aatgcataac tccggttcca gcaccgccat
    27781 ttgcaaaata tcgttgcgct ttttctcgcc gccggaaaaa ccaacgttta ccgaacgggt
    27841 taataaatct tccggcatct tcaggagagc gattttctct tccatcaaat cctgaaaatc
    27901 aaagcggtcg agcgtttcct ggccgcgata gctgcgcacc gcattaagtg ccgtttgcag
    27961 gaaaaactgg ttactgacgc cgggaatctc caccggatac tggaaggcca taaagatgcc
    28021 ttcgcctgca cgatcttccg gcgacagcgc aagcaaatct ttgcctttga actcaaccgt
    28081 gccgcccgtc acttcataat cttctcgccc ggcaagcgtt gccgataagg tacttttgcc
    28141 cgaaccgttt ggccccataa tggcgtgaac ttcgccggga cgaacgtcga ggcttaatcc
    28201 acgcaggata gctttatctt ccacgctgac gtgtaaatct ttaatactta acatgtttat
    28261 tccttatccg acgctgtgtt caagactgat ggcgaggagt ttttgtgctt caacggcaaa
    28321 ttccagcggc agttctgaga acacgtcttt gcagaaaccg ttaacaatca tcgagatggc
    28381 gtcttcttcg ctaatcccgc gttgcaggca gtaaaacagt tgatcttcac caatacgtga
    28441 tgtcgttgcc tcgtgctcca gttgtgcgct gttattgcga cattcgacat acgggaaggt
    28501 atgcgcccca caattagcgc caatcagcat tgagtcgcac tgagtgaaat tgcgcgcatt
    28561 ggttgccgtc ggcatgattt tcactaagcc gcgataactg ttctgactat gtccggcaga
    28621 gatccctttc gagataatgg tcgatttggt gtttttaccg atgtggatca tcttggtgcc
    28681 ggtatccgct tgctgatgac cgctggtcag cgccactgag taaaactcac caatggagtt
    28741 atcgccgcgc aaaatgcagc tgggatattt ccacgtaatc gctgacccgg tttctgattg
    28801 cgtccatgac attttgctgt tttcgccttc gcacaaagca cgcttggtga cgaaattgag
    28861 aataccgccg gtgttgttat cgccagggaa ccagttttgt accgtggaat atttcacctc
    28921 ggcgttttta tggatgatga cttccaccac cgccgcgtgt aactgatagc tgtcacgcac
    28981 cggagcggaa cagccttcaa tgtagctgac gtagctgtct tcgtcggcca ccagaatggt
    29041 gcgctcaaac tgcccggttt tttccgcgtt aatgcgaaaa taggtggaaa gttccatcgg
    29101 gcagcgcacg cctttaggca cataaataaa cgtaccgtca gaggctaccg ccgcattaag
    29161 cgcggcaaag aagttgtcat ttcccggcac cacagtgccg agatatttac gcaccagttc
    29221 cggatgatcg tggatcgcct caccaaagga acagaaaata attccctgct ccgccagttt
    29281 ttcgcgataa gtagtggcaa ccgaaacaga gtcgaaaatg gcatccaccg ccacctcttt
    29341 gccttcccgc acgggaacgc ccaactgctc aaacgccgcc tccacctctt tacttaaaaa
    29401 ggcgttcgcg ccagtttgct gcaccgcgcc aggttcagac gcgcaagtgt cgtcacaatt
    29461 accgcacgat ggtgctgagt agtaactgta atcctgataa ttcagcttgt cgtagtgcgc
    29521 tttcaaccag tgcggttctt ccatctccag ccatgcgcga taggcgttta gacgaaactc
    29581 cagcatccac tccggctcat tacgcttcgc cgaaattgcg cgcaccacct cttcgtttat
    29641 cccctttgcc agctcatcgg tggctaactg ggtgaagaat ccttctttat aattcagcgg
    29701 gccgccggtc caggttttga catcgtcagt tgcttcagta ttacgagaca tagtaccgcc
    29761 tataccccaa agctttcgcc acagccacat tcattctggg ctttagggtt gtgaaatttg
    29821 aatatctgat taagtccttc acgaacgaaa tcgacttccg tgccatcaat aaacggcatc
    29881 gcttgcagcg ggacaaacag cttcgcgccg tcgtgttcaa acagcagatc gtctttgtcc
    29941 ggctcgctaa cactgtcgag cacatagcca aagcccgcgc agcccgtttg cttcacgcct
    30001 aagcgcacgc cgaccatacc cggctgcttt gccaccagct cacggatgtg tatcgccgct
    30061 gcgggtgtca gcgttaagcc ttgccaggcg aaatcttgtg ggttaaaggt tcctgaatgc
    30121 atgtccatcg atttacctca cttcatcgct ttcagcgtat aacagcatgc tagtgataat
    30181 gattatcagt tcaacccagc aaacgcaggg gctttaccgt aaaacatgct ttttgcctgc
    30241 ttttaataag catagaccct gatgtgtggg ttaacaggca cgctaagaat taggtatctc
    30301 attgttagat aatggttatt atctaaggag cattaaggct gtgatgaaaa agaaaaagtt
    30361 gtattgaaaa tgactattta agagataggt aaaaaagtgc agcgttcaga aataagaaaa
    30421 cccttaagtc tgtgcgacac aggcttaagg gtttctaccc catccggcgc ttatctccgg
    30481 cactctcagt ggcttagctc ttgaaggggc gataagaata atctcataaa gctaacccgc
    30541 cgttttaaca caaactgcga ttagtattat ttttgaacaa tatcaggcgg tagataagca
    30601 gtattaagaa ggtcatcgaa cctggacgga ggttaatcca ggtcgatttg gcgaacttgc
    30661 ggcattaagt caggatcaat gcacgcccag acgccaggca aagtagattt cttcttttaa
    30721 ttcagtagaa gagagagtaa gcaggtcagc aaattcaagt tctagttgtt tcagacgttt
    30781 gagatattgg gcaggtgaaa gattgctctg gtcacggcgt aaaaattcaa tggccagctg
    30841 ggtgggatca agttgagtag acatagcatc ctcgctttta gacaagacgt gcacagtata
    30901 ccaccgttta ctgtgcagat aatgaccaaa agcaatatgc gtcacacttt tctggtgaca
    30961 acgtcacaaa atggcggtcg tcaatcgtga cgaacagcac aaacgccctt tctcatcgaa
    31021 gatttcaatc tgccagacct ggtgacgcga accgagatgc aacggtttac atatgccgcg
    31081 cacccgccct tctcgtgccg agcggacgtg attagcattg atttccagac caaccacttt
    31141 ttgctcacct tcggtacata aataaccggc aacggaaccg atactttcgg ccagtaccac
    31201 ggatgctcct ccatgcagca acccgaaagg ctgctttgtc cgcgagtcta ctggcattgt
    31261 cgcttcaagg gtgtcatcac caatatgttc aaagcgaata tccaggaacc ccaccatgtt
    31321 tccttcaccc atagcattca gtgcttccag ggtgattttc cgtttccata tcatttaata
    31381 atctccagta aagcctgcac aggatggcgt acccccgtgc cttcaacccg ttttacctgg
    31441 ctacggcagg aatatccggt cgccagacag cggttacgcg gcagtcgctg catagcctga
    31501 tgccaggata actcatagat cccgagcgaa ttttcatggt ttttcgcttc atgtccgtaa
    31561 gtccctgcca tgccgcagca acccacgctg acattttcca gtttcgcgcc aaaacgggca
    31621 aatatcgcgg cccattgtgc tggcgcgccc ggcaaagcgg taacctcggt acagtgacca
    31681 aagaaatacc atgattcacc gctgactgta gccactggat gtgactcaag tgcgcttgcc
    31741 agccattcat tcgcaagtaa gacgttaaac tcgccacgtt cctcgcccag cgccagttta
    31801 tattcatcgc gataacaaag taccagcgcc ggatcgacgc ccaccattgg catcccaagc
    31861 ttagccatac ggttgaggaa atccgccgtc tttttcgccg tcttcgcaaa acgattaaga
    31921 aagcctttga tatgctgggc tttgccattt ggcgaaaatg gcagtaacac aggctggaaa
    31981 cctaattttt caaccagacg gacaaaatcc gccaccactt gcgcatcgta atagctggta
    32041 aaggggtcct gcaccaccaa caccgtgcgc gctttctgct ctgcattgag agcttcaagc
    32101 tgttccagcg tcatgtttgc cgagcgatgc cccaccattt gttgttgtag cgaggggacc
    32161 gacagcagtg gcaaatcaac catgccgata tgtttttccg agagtttgcg caccagcggc
    32221 tggttaatga agaagttaaa ggttttcggc gcgcgtgcca tcagcggtgc gtagctctcg
    32281 accgtagcga cgaggtggtc gcgcagcggg cgtaaataac gggtgtgata gagctgcagg
    32341 aaacgagagc gaaactccgg cacatcaatt ttgatggggc actgggtcga acacgcttta
    32401 caggccagac agcccgacat cgcctctttg acttcgtgtg agaagtcata ttcgccttta
    32461 ttcgcatgcc agctattgcg cgtgcgggca attaacgtcc gtaaactgac gccggattca
    32521 ggcagttctt gttccagttt gagtggatca acgccgcgat ccgccaacaa acgcagccat
    32581 tcacgcacca gcgttgcgcg ccctttcggt gaatgaatcc ggttctgggt gatcttcatc
    32641 gacggacaca tcggactacg ggcatcaaag ttgaagcata aaccgttgcc gttacactcc
    32701 atcgcaccgc gccactgctg gcgtaccgca atggggatct gccgatcgaa tgtaccgcgc
    32761 ttcaccgcgt ccactttcat catcggcgca tcgagacctt ctggcgggca aatcttccct
    32821 gggttgagtc ggttatgcgg gtcaaatgcc gctttcactt tgcgcagttc tgcaaaaagt
    32881 tcctcaccga aaaacgccgg gctgtattca gcgcgaaaac ctttgccgtg ctcgccccac
    32941 aacaaaccac cgtatttcgc agtcagcgcc accacgtcat cagagatttg cttcatcaaa
    33001 atctcttgtt gcggatcgca catatccagt gccggacgga cgtgcaaaac acctgcgtcg
    33061 acgtgaccga acataccgta gcttaagccg tggctgtcga gcagcgcgcg aaattcagca
    33121 atataatcgg ccagatgttc cggcggtacg caggtatcct cagcaaacgg aattggctta
    33181 gcggcacctt tggcattgcc aagcagacca acggcttttt tgcgcattgc atagatacgt
    33241 tcaacccccg ccagctcgcg gcacacctgc cagccaatca cacctgcctg ttggctggca
    33301 atcagctcat caagccgcac acagagtgca ttaactcgct cgtcaatcag cgcctcatca
    33361 tcaccagcaa attccacaat gttcagcccg agcatctctt tgtcaggcac atcggtaatc
    33421 aactcgctga cggaatgcca gacaatatct tcccgcgcca gattcagcac ttttgagtcc
    33481 accgtctcta ccgaaagcgc ccgcgcctca accataaacg gcgcgttacg cagcgcggag
    33541 tcaaaagagt catatttgac gttcaccaga cggcgcactt taggcaaggg cgtaatatcc
    33601 agccgcgctt cggtaataaa ggccagcgtc ccttctgaac ccgtcagaat gcgcgtcagg
    33661 tcgaactcgg tcatctcatc gttaaagaca tgacgcagat cgtaaccggt aagaaagcgg
    33721 ttaagtttgg ggaagttgtc gatgattaac tggcgttgca gacggcaacg ttgataaacc
    33781 gtgttataaa ttcgcccgat tgtggtattg gttttaccca gcgtttccgc cagttcgacg
    33841 ggtaaaggtt gcgtatcgag aatatcgccc cccaacaaca ccgcgcgtac gccaagtacg
    33901 tgatctgacg ttttgccata gaccagcgat ccctgaccgg atgcatcggt attgatcatc
    33961 ccgccgagcg ttgcccggtt gctggtcgaa agttctggtg caaaaaagta gccgaacggt
    34021 ttcaggtact gattgagttg atcttttatc accccggcct caacgcgcac ccagccctct
    34081 tcagggttaa tttcgatgat gcggttcata tggcgggaca tatcaacaat aatcccctgg
    34141 ttgagcgcct gaccgttagt gccggtgccg ccgccgcggg gggtaaagat cagcgatgaa
    34201 tagcgttcct gcgcggcaag acgggcgatc agcgccacat ctgcggttga acgcggaaat
    34261 accaccgcat cggggagaag ttggtaaata ctgttgtcgg tcgacattgt cagacgatcg
    34321 gcataacttg tcgccgtatc gccggtaaaa ccttgttgct ccagctcttg caaaaaatta
    34381 agcaccagtt gaacgacgcc gggtgcctgg gaaatctgtg gaatcattat attgaccctt
    34441 tcctgcggtc tgtgatgtag gtcgatacac tattctttca ggcggctgca atagcgcact
    34501 gaaaggtgat gtttatttac tctatggatt tcgagttgca ggaaggcggc aagcgagtga
    34561 actccaggag cttacaatag taagtgactg gggtgaacga acgtagccgc agcgcatgca
    34621 acttgaaata cgacgagtaa atcgtttgcg tgttgcctga gttgttgtac cacatttttt
    34681 tctaacacgc ccatcagaat taagggcaga atcggcctgt taaaaaccgc tgaaattgct
    34741 catcattatg caggtgagtt tcgcgtgttc acgtcgcgtc gacgatttga cgcacaaaaa
    34801 aggtgaaaag tagttatggt aaatgttcgt cagcccaggg atgtcgcaca aattctgctt
    34861 tcggtgctgt ttttagccat catgattgtg gcatgtctgt ggattgttca accctttatt
    34921 ctcggctttg catgggccgg tacggtggtt atcgccacct ggccggtatt gttacgtttg
    34981 caaaagatca tgtttggccg ccgctctctc gccgttctgg tgatgacgct gttattagtg
    35041 atggtgttta tcatccctat tgctttgctg gttaacagta tcgtcgacgg cagcggcccg
    35101 ctaattaaag ccatttccag cggcgacatg acgttacccg atctggcgtg gcttaatacc
    35161 attccggtga ttggcgcgaa gctgtatgca ggctggcaca acttgctgga tatggggggt
    35221 acggcgatca tggcgaaagt ccgcccttat attggcacca ccaccacctg gttcgttggg
    35281 caggcggcgc atatcgggcg ctttatggtg cattgtgcgc tgatgcttct cttcagtgcc
    35341 ctgctgtact ggcgcggtga acaggtggca caaggcattc gccattttgc aacccgtctg
    35401 gcaggcgttc gcggagatgc cgccgtgctg ctggcggcac aggctatccg cgcggtggcg
    35461 ctgggtgtgg tggtgacggc gttagtacag gcagtgcttg gcagtatcgg cctcgccgta
    35521 tccggcgtac cttatgcaac gttgctaacg gtgttaatga tcctctcctg ccttgtccag
    35581 cttggcccgt tgccggtact gattccggcg attatctggc tctactggac cggcgatacc
    35641 acctggggaa cggtattgtt agtgtggagc ggtgtggttg gcacgctgga taacgtcatc
    35701 cgcccaatgt taattcgcat gggtgccgat ttaccgctga tcctgatcct ctctggcgtt
    35761 attggtggtt tgattgcttt cgggatgatc ggtctgttta ttggtccggt tctgttagcc
    35821 gtttcctggc gtctgtttgc cgcgtgggtg gaagaagtcc cgccgccgac ggaccaaccg
    35881 gaagaaattc tcgaagaact tggcgaaatc gagaaaccga ataagtaatt tctcatcagg
    35941 cggctctgcc gcctgattgt taatcaccgc taattgatat ttgcttgctc ttccccatat
    36001 tttcctgctt accgccattc agct
//
LOCUS       DAJTZL010000023        35777 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.023, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000023 DAJTZL010000000
VERSION     DAJTZL010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35777
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.023"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(1..581)
                     /locus_tag="OO544_000986"
                     /pseudo
     CDS             complement(1..581)
                     /locus_tag="OO544_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
     gene            complement(810..1730)
                     /gene="malM"
                     /locus_tag="OO544_000987"
     CDS             complement(810..1730)
                     /gene="malM"
                     /locus_tag="OO544_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HCR6766648.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTSDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPVPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            complement(1882..3222)
                     /gene="lamB"
                     /locus_tag="OO544_000988"
     CDS             complement(1882..3222)
                     /gene="lamB"
                     /locus_tag="OO544_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HCR6766649.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            complement(3294..4409)
                     /gene="malK"
                     /locus_tag="OO544_000989"
     CDS             complement(3294..4409)
                     /gene="malK"
                     /locus_tag="OO544_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HCR6766650.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            4774..5964
                     /gene="malE"
                     /locus_tag="OO544_000990"
     CDS             4774..5964
                     /gene="malE"
                     /locus_tag="OO544_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HCR6766651.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVAVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            6118..7662
                     /gene="malF"
                     /locus_tag="OO544_000991"
     CDS             6118..7662
                     /gene="malF"
                     /locus_tag="OO544_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HCR6766652.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ECAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGEIGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGADPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLLFLLVGALAIVNLKA
                     TRMKFD"
     gene            7677..8567
                     /gene="malG"
                     /locus_tag="OO544_000992"
     CDS             7677..8567
                     /gene="malG"
                     /locus_tag="OO544_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HCR6766653.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     AIGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            8939..10414
                     /gene="xylE"
                     /locus_tag="OO544_000993"
     CDS             8939..10414
                     /gene="xylE"
                     /locus_tag="OO544_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HCR6766654.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKIAAVLFFISGVGS
                     AWPELGFTSINPDNTVPIYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGWRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQAESILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQAPGIVALLSMLFYVAAFAMSWGPVCW
                     VPLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(10458..10868)
                     /gene="psiE"
                     /locus_tag="OO544_000994"
     CDS             complement(10458..10868)
                     /gene="psiE"
                     /locus_tag="OO544_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HCR6766655.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(10994..11137)
                     /locus_tag="OO544_000995"
     CDS             complement(10994..11137)
                     /locus_tag="OO544_000995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6766656.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPAWVRFVLPDAPPGAAYP
                     AYG"
     gene            11138..11416
                     /locus_tag="OO544_000996"
     CDS             11138..11416
                     /locus_tag="OO544_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6766657.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            complement(11463..13559)
                     /locus_tag="OO544_000997"
     CDS             complement(11463..13559)
                     /locus_tag="OO544_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HCR6766658.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYIDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETTLAQKRVEPVVPQSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTIGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDAYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(13559..14296)
                     /locus_tag="OO544_000998"
     CDS             complement(13559..14296)
                     /locus_tag="OO544_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HCR6766659.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSTDDAAAINALRQ
                     QIQALEVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISHPGNQP
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            complement(14293..14931)
                     /locus_tag="OO544_000999"
     CDS             complement(14293..14931)
                     /locus_tag="OO544_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HCR6766660.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            complement(15045..15287)
                     /locus_tag="OO544_001000"
     CDS             complement(15045..15287)
                     /locus_tag="OO544_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6766661.1"
                     /translation="MKKVLYGIFAISALAATSVWAAPVQVGEAAGAAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            complement(15749..17398)
                     /gene="pgi"
                     /locus_tag="OO544_001001"
     CDS             complement(15749..17398)
                     /gene="pgi"
                     /locus_tag="OO544_001001"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HCR6766662.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     gene            17923..19272
                     /gene="lysC"
                     /locus_tag="OO544_001002"
     CDS             17923..19272
                     /gene="lysC"
                     /locus_tag="OO544_001002"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HCR6766663.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
     gene            19524..19796
                     /locus_tag="OO544_001003"
     CDS             19524..19796
                     /locus_tag="OO544_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HCR6766664.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(19929..20801)
                     /gene="rluF"
                     /locus_tag="OO544_001004"
     CDS             complement(19929..20801)
                     /gene="rluF"
                     /locus_tag="OO544_001004"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HCR6766665.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            21149..21986
                     /locus_tag="OO544_001005"
                     /pseudo
     CDS             21149..21986
                     /locus_tag="OO544_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313031.3"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
     gene            22056..22862
                     /locus_tag="OO544_001006"
     CDS             22056..22862
                     /locus_tag="OO544_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR6766666.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEVHKGYQFWPTDISSAKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            22872..23279
                     /locus_tag="OO544_001007"
     CDS             22872..23279
                     /locus_tag="OO544_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIA"
                     /protein_id="HCR6766667.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGDANVEAVEFVPGENAGDIV
                     AKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATAEEDF"
     gene            23279..23773
                     /locus_tag="OO544_001008"
                     /pseudo
     CDS             23279..23773
                     /locus_tag="OO544_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
     gene            23839..24636
                     /locus_tag="OO544_001009"
     CDS             23839..24636
                     /locus_tag="OO544_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HCR6766668.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWRKAEPQLQATASTALDQLDD"
     gene            24647..25471
                     /locus_tag="OO544_001010"
     CDS             24647..25471
                     /locus_tag="OO544_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCR6766669.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRRHLVFFNTTPAVCGPVIGVTAAMEEARANGAEIDDGTINGIKVG
                     LMGPLAGVGDPLVWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQL
                     GFRKGVNIVSDMGGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGST
                     VIMTVQNILDQLCPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            25523..26751
                     /gene="sorE"
                     /locus_tag="OO544_001011"
                     /pseudo
     CDS             25523..26751
                     /gene="sorE"
                     /locus_tag="OO544_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
     gene            27134..27823
                     /gene="pepE"
                     /locus_tag="OO544_001012"
     CDS             27134..27823
                     /gene="pepE"
                     /locus_tag="OO544_001012"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HCR6766670.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TCDDYTAKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(27914..29545)
                     /locus_tag="OO544_001013"
     CDS             complement(27914..29545)
                     /locus_tag="OO544_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HCR6766671.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(29765..33448)
                     /gene="metH"
                     /locus_tag="OO544_001014"
     CDS             complement(29765..33448)
                     /gene="metH"
                     /locus_tag="OO544_001014"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HCR6766672.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDGLV
                     VAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFETLGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAEAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGEDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWEVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNEPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTANAQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            33648..34472
                     /gene="iclR"
                     /locus_tag="OO544_001015"
     CDS             33648..34472
                     /gene="iclR"
                     /locus_tag="OO544_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HCR6766673.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
     gene            34789..35777
                     /gene="espL4"
                     /locus_tag="OO544_001016"
                     /pseudo
     CDS             34789..35777
                     /gene="espL4"
                     /locus_tag="OO544_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL4"
BASE COUNT         8598 a         9230 c         9100 g         8849 t
ORIGIN      
        1 actatgacca agttgactct gcgagaagtc aactgtatca agaatatttt ggctaaaatc
       61 aacacattcg ccactcgttt tgagaaaaat cacattgtgt aaacgtcgac atttaaatat
      121 gacattatca actgttgcct tagtaaaatt gcaattctca atttctttac attcgaaagt
      181 tacatgttgt agatttgatg ataaaaaaga caccttagaa ataacgtcac ctttaaaatg
      241 acaatttatt aaatcaaaat cagacaggtc gatatacccc tgcgcatctt tatccatcag
      301 aaaattacaa gcgcattcta ttttttcatt tctgtagaat tttacactta tagtatcatt
      361 actaattgag acttcacgta acatatttat tgcatgattt tttcgttccg ttgctttata
      421 tttaaaaata tatcactgat ataaataaag ttatctctgt ttaaacctgt caccccattg
      481 aaattacgta gtaaatcaag atctactata ttatttcctt gaaccacatc atgtttcatc
      541 aaagagttat tgccgccaga atttgtgata tgacctatca taaaacctct gtaatacaga
      601 aataaaataa cctggaaaag tagcctgagc gtccagacac taccggcgaa aggaaaagaa
      661 tctctgataa ggcattgaga taatggacat tcttagtgaa gaagaagtga agtactataa
      721 aaaatcgctc atatcccagg aaggaagcat aaaaaaaggt gcgccaggag acgcaccagt
      781 tgttgcaaaa tcagcactgt ggggcgtaat taccccttgc cttttacact gctgataaag
      841 gtggaacggg cagatgtcga tcccaggcgt tcagcttcat caagcagttt taacgcctta
      901 tcaacatcac ctttcgcgac agcgtttttg atcgcggtat taaaataact ttccgtgtcg
      961 ttgagcatcg gctcgctttt cttcaccggc accggagcag gtgcagccac agctggtgcc
     1021 gccgtgttgc ctaccgtaac cggagctgga gcggaagaac caaatagtgg tcctaccaac
     1081 acgctggagc tggagttcgt tttgactttc agtttcagta agccatcgct ggtatgacga
     1141 gcaaccggat cgggaatatc cgggatcgag ttaccgacgc ctttggcata ggctttagcc
     1201 gggtcgagca gttgggtcgt ctgctggaga tctttttccg tggtaaagac cagaacataa
     1261 agtttttgct gccccaacgc cggtgtcagg cgcataacgc cttccagccg atctgcactc
     1321 atcacgcctg gttcctggta ggtgaaataa ctgctgggga agaaggctga tggggtcatg
     1381 ttctgatcaa gaatcagcac gttcggcgca aaaacgctgg tttgtttgtt cacttcgctg
     1441 gtcagcgtca gggtcagttc gccaatgttt gccgggacgc tgtacgcagc aaccggacca
     1501 ctgatgccgg gaacgttcag ttgttggccg ccggtcgcca gttgggtggt ctgggtttta
     1561 gattgatcga ccggtgtcca ggtgagttgt tgcagcgcgg cagatggaat ggctggcgcg
     1621 tcgctggtgt tttgcggtac gtagttaaca tcggcaaggc taattccagg cgcgcttgcc
     1681 agtaaccctg ctgataaaca gagggcgatg agacttttat tcattttcat tgttttcacc
     1741 tcaaaatctg gagctcagcg gtagccaggc aatagcgcgc taaacccgat aatcagaggg
     1801 gctttcgccc ctttcagata atgacaacct gtttttatgc cggatgcggc gtaaacgcct
     1861 tatccggccc aggttttgct attaccacca gatttccatc tgggcaccga aggtccactc
     1921 gtcgctgtcg ccacgaccga agctgccgcc gttgaaatca gcaggaacgg ctttgccgta
     1981 gttcgggtta accttgctat cgccgttgta gtcgtaaccc catttctcat cccacttggc
     2041 gtaggttgcg aagacacgaa tagccgggcg tgaccagatg ctgtcgccag cctgccattg
     2101 ttgtgcgagg gtaattttgt actgattgtt cttgtcgccg gtgcgctggg attcgacgtt
     2161 gtcgtagccg atttccatca cggtgctcat gattggcgtc cacttgtaca tcgggcgaat
     2221 accgacggtc caccacttgg tgccgttgtc gttatcccag ttgatatcct ggtacatacc
     2281 cacgtacatc atgtcccagt tgtcgcccat ggagatcgca ccgtggtcga ggatacgcag
     2341 catgtgaccg ttgttgttga tattgtaggc aaatttttcg ttatcaaacg cgacgccaga
     2401 accctgcgac agacctttac cctgcgaggt catcgagtca gtagcgtact gaacaacaaa
     2461 cttgttaaag cccttcagga cactctgagt atgttcagca gtgaataacc agccgtcttt
     2521 cgatgcgcca tcaaccagac gatagttatc acgcaggttg gcacgaccgt agtcgacacc
     2581 cagttctaat gtgccgcccg ggttgatttc catctgcgct aaacgcacat cgaaaacgtc
     2641 gttcgcggtt tcgttggtat agtcataaat attgttgctg gcaaaagagg aagaaccacc
     2701 agcttcagag gagcgggttg ctgccagaga gagtttaccg aagccaacat cgatgttttc
     2761 cagaccggca ccaggaccag aaatatccca gtagtagaag tcgatcatat gaacgtcatg
     2821 acgttggtag aagcgcttac ctgcccagat ggtggaacct ggcagccatt cgatcaggtt
     2881 tttaccctgc acgtttgctt cacggaaggc cggatcggta gcttcccagt cattctgttg
     2941 cgcgacggaa taggccacgt tagtgtcgaa atagaagctc ttatcgccct ctttccacac
     3001 ttcctgaccc aattttaatt cagcataagt ttcacattcg ttgccaagac ggtatttact
     3061 ttgagcaccg gtagtctgga aacactgttg ttcaccgccg ctacctgtcc agccaatacc
     3121 ggaacgtgca tagccgtgga aatcaacagc cattgcctga gcagacatta cgcccgctgc
     3181 gacggcaacc gccagaggaa gtttgcgcag agtaatcatc attctatctc ctgagtcatt
     3241 gcttttcttt tttcacatca cctgtgacag gctttgtgtg ttttgtgggg tgcttaaacg
     3301 cccggctcct tatgcagtcg acgacatgca gtgccatcct cacggaacag atggcaacgc
     3361 tctggcggca ggccgatagc gaatgtggca ccttcttcta ccaacaccac gtcgttctgg
     3421 cggtacacca ggttttgacg aatggaaggg atctggatat ggatttgggt ttcgttgccg
     3481 agttgctcga cgacctgaac ttcaccctca aggatgacgt cagcgatatc actcggcagt
     3541 agatgttccg ggcgaatacc cagcgacata ttggctccaa cctggacatc acggctttca
     3601 actggcagcc agacttgctg acgatttggc atcggcagct ccacctgcac ttgatcgatt
     3661 gcggtggcgg tcacttttac cggcaggaag ttcatctttg gcgaaccgat aaatccggcg
     3721 acaaaacggt ctgccggata gtggtacagc tccagcggtt tcccaacctg cgccacgcga
     3781 ccggcgtcca gcaccacgat tttgtcggcc agcgtcatcg cttcgacctg atcgtgggtg
     3841 acgtaaatca ttgtgcggcc caggcgttta tgcagacggg agatttcgat acgcatttgc
     3901 acacgcagtg cagcatcgag gttggagagc ggttcatcga gcaaaaatac gcttggctcg
     3961 gccaccagcg tacggccaat cgccacacgc tgacgctgac caccggagag cgctttcggt
     4021 ttgcgatcca gcaaatgcgc cagttgcagc acttccgcca cctggttgac gcgttggtta
     4081 atcacctctt tttttgcgcc agccagtttc aggccaaatg acatgttttc tgctactgac
     4141 aggtggggat agagcgcgta agactgaaac accataccaa cgccgcgttc tgctggcgga
     4201 gtgtcattca tccgtttctc accgatgaac aggtcgccgc tggtgatcgt ctcaagcccg
     4261 gcaatcatgc gcagtaaagt cgatttaccg cagccagacg gtccgacaaa caccacgaat
     4321 tcaccttcat ggatatcgag attgatatct ttcgatacca cgacctcgcc ccaggctttc
     4381 gttacatttt gcagctgtac gctcgccatg cccttctccc tttgtaacaa cctgtcatcg
     4441 acagcaacat tcatgatggg ctgactatgc gtcatcagga gatggcttaa atcctccacc
     4501 ccctggcttt tttatggggg aggaggcggg aggatgagaa cacggcttct gtgaactaaa
     4561 ccgaggtcat gtaaggaatt tcgtgatgtt gcttgcaaaa atcgtggcga ttttatgtgc
     4621 gcatctccac attaccgcca attctgtaac agagatcaca caaagcaacg gtggggcgta
     4681 ggggcaagga ggatggaaag aggttgccgt ataaagaaac tagagtccgt ttaggtgttt
     4741 tcacgagcac ttcaccaaca aggaccatag attatgaaaa taaaaacagg tgcacgcatc
     4801 ctcgcattat ccgcattaac gacgatgatg ttttccgcct cggctctcgc caaaatcgaa
     4861 gaaggtaaac tggtaatctg gattaacggc gataaaggct ataacggtct cgctgaagtc
     4921 ggtaagaaat tcgagaaaga taccggaatt aaagtcaccg ttgagcatcc ggataaactg
     4981 gaagagaaat tccctcaggt tgcggcaact ggcgatggcc ctgacattat cttctgggcg
     5041 cacgaccgtt ttggtggcta cgctcaatct ggcctgttgg ctgaaatcac cccggacaaa
     5101 gcgttccagg acaagctgta tccgtttacc tgggatgccg tacgttacaa cggcaagctg
     5161 attgcttacc cgatcgctgt tgaagcgtta tcgctgattt ataacaaaga tctgctgccg
     5221 aacccaccaa aaacctggga agagatcccg gcgctggata aagaactgaa agcgaaaggt
     5281 aagagcgcgc tgatgttcaa cctgcaagaa ccgtacttca cctggccgct gattgctgct
     5341 gacgggggtt atgcgttcaa gtatgaaaac ggcaagtacg atattaaaga cgtggccgtg
     5401 gataacgctg gcgcgaaagc gggtctgacc ttcctggttg accttattaa aaacaaacac
     5461 atgaatgcag acaccgatta ctccatcgca gaagctgcct ttaataaagg cgaaacagcg
     5521 atgaccatca acggcccgtg ggcatggtcc aacatcgaca ccagcaaagt gaattatggt
     5581 gtaacggtac tgccgacctt caagggtcaa ccatctaaac cgttcgttgg cgtgctgagc
     5641 gccggtatta acgccgccag tccgaacaaa gagctggcga aagagttcct cgaaaactat
     5701 ctgctgactg acgaaggtct ggaagcggtc aataaagaca aaccgctggg tgcagtggcg
     5761 ctgaagtctt acgaggaaga gttggcgaaa gatccacgta ttgccgccac catggaaaac
     5821 gcccagaaag gtgaaatcat gccgaacatc ccgcagatgt ccgctttctg gtatgccgtg
     5881 cgtactgcgg tgatcaacgc cgccagcggt cgtcagactg tcgatgaagc cctgaaagac
     5941 gcgcagactc gtatcaccaa gtaatgctgt gaaatgccgg atgcggcgtg aacgccttgt
     6001 ccggcctaca aaactgaaac gcatgtaggc ctgataagac gcatcagtgt cgcatcaggc
     6061 agttgttgtc ggataaggcg tgaaagcctt atccgtcctg gaatgaggaa gaaccccatg
     6121 gatgtcatta aaaagaaaca ttggtggcaa agcgacgcgc tgaaatggtc agtgctaggt
     6181 ctgctcggcc tgctggtggg ttaccttgtt gttttaatgt acgcacaagg ggaatacctg
     6241 ttcgccatta ccacgctgat attgagttca gcggggctgt atattttcgc caatcgtaaa
     6301 gcctacgcct ggcgctatgt ttacccggga atggctggaa tgggattatt cgtcctcttc
     6361 cctctggtct gcaccatcgc cattgccttc accaactaca gcagcactaa ccagctgact
     6421 tttgaatgtg cgcaggaagt gttgttagat cgctcctggc aagcaggcaa aacctataac
     6481 tttggtcttt acccggcggg cgatgagtgg caactggcgc tcagtgacgg cgaaatcggc
     6541 aaaaattacc tctccgacgc gtttaaattt ggcggcgaac aaaaactgca actgaaagaa
     6601 acgaccgccc agcccgaagg cgaacgcgcg aatctacgcg tgattaccca gaatcgtcag
     6661 gcgctgagtg acattaccgc cattctgccg gatggcaaca aagtgatgat gagctccctg
     6721 cgccagtttt ctggcacgca gccgctctac acactcgacg gtgacggcac gttgacgaat
     6781 aatcagagcg gcgtgaaata tcgtccgaat aaccaaattg gcttttacca gtccattacc
     6841 gccgacggca actggggtga tgaaaagcta agccccggtt acaccgtgac tactggctgg
     6901 aaaaacttta cccgcgtctt caccgacgaa ggcattcaga aaccgttcct cgccattttc
     6961 gtctggaccg tggtgttctc gctgatcact gtctttttaa cggtggcggt cggcatggtt
     7021 ctggcatgtc tggtgcagtg ggaagcattg cgcggcaaag cggtctatcg cgttctgctg
     7081 attctgccct acgcggtgcc atcattcatt tcaatcttga ttttcaaagg gttgtttaac
     7141 cagagcttcg gtgaaatcaa catgatgttg agcgcgctgt ttggcgtgaa gcccgcctgg
     7201 ttcagcgatc cgaccaccgc ccgcacgatg ctaattatcg tcaatacctg gctgggttat
     7261 ccgtacatga tgatcctctg catgggcttg ctgaaagcga ttccggacga tttgtatgaa
     7321 gcctcagcaa tggatggcgc agatccgttc cagaacttct ttaagattac gctgccgctg
     7381 ctgattaaac cgctgacgcc gctgatgatc gccagcttcg cctttaactt taacaacttc
     7441 gtgctgattc aactgttaac caacggcggc ccggatcgtc ttggcacgac cacgccagcc
     7501 ggttataccg acctgcttgt taactacacc taccgtatcg cttttgaagg cggcgggggt
     7561 caggacttcg gtctggctgc agcaattgcc acgctgctct tcctgctggt gggtgcgctg
     7621 gcgatagtga acctgaaagc cacgcgaatg aagtttgatt aagggagata acaaaaatgg
     7681 caatggtcca accgaaatcg caaaaagcac gtttgtttat tactcacctg ctactgctac
     7741 tttttatcgc ggcgattatg ttcccgctgc tgatggtcgt cgctatctca ctgcgccagg
     7801 ggaactttgc gatcggcagc ctgatcccgg agcaaatctc ctgggatcac tggaaactgg
     7861 ctttaggttt tagcgtggaa caggctgatg gtcgcattac gccaccgcca ttcccggtac
     7921 tgctgtggct atggaactcg gtaaaggtcg ccgggatttc cgcgattggc attgtcgcgc
     7981 tctctaccac ctgcgcctac gctttcgccc gtatgcgctt tccaggcaaa gcgacgctgc
     8041 tgaaaggaat gctgattttc cagatgttcc cggcagtact ttcactggtc gcgttgtatg
     8101 cgttgtttga tcgtctgggt gagtacattc cattcattgg cctgaatact cacggcggcg
     8161 taattttcgc gtatctgggt gggattgcgc tgcatgtctg gaccatcaaa ggctatttcg
     8221 aaaccatcga tagttcgctg gaagaagcgg ctgcgctgga tggtgcgaca ccgtggcagg
     8281 ccttccgcct tgtcctgttg ccgctgtcag taccgattct ggcggtggta ttcatcctgt
     8341 cgtttatcgc tgccattact gaagttccgg tcgcgtcgct gttactgcgt gacgtaaaca
     8401 gctacaccct ggccgtgggg atgcagcaat acctcaaccc gcaaaactac ctgtggggtg
     8461 actttgccgc cgctgccgtg atgtctgcat taccgatcac catcgtcttc ttgctggctc
     8521 aacgctggct ggtcaacggc ctgacggcag gtggtgtgaa aggttaaaga tgttgttctg
     8581 ccaatgttat gccgctgcac cctcaactta cgttatccca acttgtgact gttattcggc
     8641 gctccacgga gcgccttttt ttctttcgtc tgcaatctga atcgttcgcc ggttaatatt
     8701 tccatcatag agcttattat ttttacgtta tttgttttcc cacttacgat aattctcttt
     8761 cgtgctctga gtcacggcaa tagtattgtt ttcatcaatt tttgataatt atcacaatta
     8821 agatcacaga aaagacatta cgtaaacgca ttgtaaaaaa tgataattgc cttaactgcc
     8881 tgacaattcc aacatcaatg cactgataaa agatcagaat ggtctaaggc aggtctgaat
     8941 gaatacccag tataattcca gttatatatt ttcgattacc ttagtcgcta cattaggtgg
     9001 tttattattt ggctacgaca ccgccgttat ttccggtact gttgagtcac tcaataccgt
     9061 ctttgttgct ccacaaaact taagtgaatc cgctgccaac tccctgttag gattttgcgt
     9121 agccagcgct ctgattggtt gcatcatcgg cggtgccctc ggtggttatt gcagtaaccg
     9181 cttcggccgt cgtgattcac ttaagattgc tgctgtcctg ttttttattt ctggtgtagg
     9241 ttctgcctgg ccagaacttg gttttacctc tataaacccg gacaacacag tacctattta
     9301 tctggcaggt tatgtcccgg aatttgttat ttatcgtatt attggcggta ttggcgttgg
     9361 tttagcctca atgctttcac caatgtatat tgcggaactg gctccagctc atattcgcgg
     9421 aaaactggtt tcatttaacc agtttgcgat tattttcggg caacttttag tttactgcgt
     9481 aaactatttt attgcccgtt ccggtgatgc cagctggctg aatactgacg gctggcgtta
     9541 tatgtttgcc tcggaatgta tccctgcact gctgttctta atgctgctgt ataccgtgcc
     9601 agaaagtcct cgctggctga tgtcacgcgg caagcaagaa caggcggaaa gtatcctgcg
     9661 caaaattatg ggcaacacgc ttgcaactca ggcagtacag gaaattaaac actccctgga
     9721 tcatggccgc aaaaccggtg gtcgtctgct gatgtttggc gtgggcgtga ttgtcatcgg
     9781 cgttatgctc tccatcttcc agcaatttgt cggcatcaat gtggtgctgt actacgcgcc
     9841 ggaagtgttc aaaacgctgg gggccagcac ggatatcgcg ctgttgcaga ccattattgt
     9901 cggagttatc aacctcacct tcaccgttct ggcaattatg acggtggata aatttggtcg
     9961 taagccactg caaattatcg gcgcactcgg aatggcaatc ggtatgttta gcctcggtac
    10021 cgcgttttac actcaggcac cgggtattgt ggcgctactg tcgatgctgt tctatgttgc
    10081 tgcctttgcc atgtcctggg gtccggtatg ctgggtaccg ctgtcggaaa tcttcccgaa
    10141 tgctattcgt ggtaaagcgc tggcaatcgc ggtggcggcc cagtggctgg cgaactactt
    10201 cgtctcctgg accttcccga tgatggacaa aaactcctgg ctggtggccc atttccacaa
    10261 cggtttctcc tactggattt acgggtgtat gggcgttctg gcagcactat ttatgtggaa
    10321 atttgtcccg gaaaccaaag gtaaaaccct tgaggagctg gaagccctct gggaaccgga
    10381 aacgaagaaa acacaacaaa ctgctacgct gtaatcttcc tgtccagcac gccgcgccag
    10441 ttcggcgtgc tttcttttta ctcccgcttc agccgtttcg aattacacag ccacagagtg
    10501 atcaccagta acaggatcgc agccgagtag atcaacacat ccagtggcga tttatgatcg
    10561 acgatgatca agcgcacaat ggcggtgatc ccaatataga caaagtaacg tagcgggaag
    10621 tgaaaaccgg actgaaagta cttcacaatc agcgcgataa attcgaaata gagaaagtaa
    10681 accaccagtc cttctaccag ctcatatttg ctggtttgtt ctggcgcgaa cagcacatct
    10741 gccagatgca ccgtttcttt gccgaggaag acaaccaaaa tcaggccgag gcacagcagg
    10801 ccaagattga gtacggtctg caaaatggtg gagataaact ccacgcgcgg acgagagagt
    10861 gacgtcatag cagtacctcc ttcacagtgg cgagcttcct gtataacgca aaaatgtgac
    10921 ggagatctat attttttgtg tatgttttgt tcaacctgga tttggagcgc gcctccacca
    10981 aagccggata tacctacccg taggccggat aagccgcgcc tggcggcgca tccggcaaga
    11041 caaaccgcac ccaagccggt aattcaaaaa tcacccattc aggtgattcc cagccaccag
    11101 caatgcgcga aaaatcgaca atgttcctct ggaacaaatg aaacgtaacc tgataaaggt
    11161 ggtaaaaatg aagccttatt ttgctgcttt gatgttatca gtctctgttc ttcctgctta
    11221 tgctggtccg ctcggtactg ctgataaagc cgacctaccg caaagcaatg tctccagccc
    11281 aatgatggcc cagtcgctca ggcaaccaga cctgcaaccg atatcgacgg acaggaagac
    11341 agaatgtttt cgcctttaca caccagatcg caaaccgggg gtgaactgcg ttcctgatgg
    11401 ctcaacaggc cattaagaat gagggtgcca gtgattgcga aaactggcac ctttcccatg
    11461 acttagcgga aattgacgct acggtcgctg gtcatatcat acaactggaa cttacgccca
    11521 agttgctgac caccgtcacg cgtcagcggc gtccagccaa tcgccgcacg gctgcgtgtc
    11581 gggccagacg agaagagatc caacggtacc gagacataca cgcctttggt gaagtcccct
    11641 tcgccgtact cctcttttga aacattcgtg atcgtggcat agccacccac caccacgccg
    11701 ctatcaaagc gtttggcgat ctccagcgtg ccgcctttat cccctgccag atactgcccg
    11761 acgctggctt tcactaacac gtcctgggcg aaagatggcg tccagtaggc ggtcagatgt
    11821 ccggttttca cgctgtagtc ggtgaatttc atcatatctt ttgcactacg ccagtcgcgc
    11881 tgtttaacgt agttggcatc cagaccaaac gcccagttgc tgtccagcgg acgatacaac
    11941 acttcagccc ccgcaccgcc aaacatggtt tcgagataac ctccatagac ctgaccgtag
    12001 aagccgttgc ccagatgctg gaagtagttg gcttgcaggt tattcacata ggcatcgttc
    12061 tgcacatact cgcgcacatg ggtacgaacg cgcggtaagt gcgagtcctg tggcggattg
    12121 gtgtagttaa acttgtcgta gttgttggca agatttgcaa acaggctgcc gatggtcagc
    12181 agatggtccg tcagccacaa atctgccgtt cccatcacgc ccagctgata catgtaaaag
    12241 ttttccgggc caccgaccga ctggttcagc accggatcga tatggaaatc aaagcgtgat
    12301 ttgtcgatat accagccctg ctcggtggac tgcggaacca ctggctcgac gcgtttttgc
    12361 gccagcgtcg tttcgtggcc caacggctct ccggcgagat gatttttcag gctggcgaca
    12421 tcggtttccg tcgtcacctg cggcatgtta aggcgatttt ccgtaatgcg gatcgttttg
    12481 atcccatccg gcagatcgtt catcacgatc cgattagcac ggatgatccc ttcgcgcgaa
    12541 tcacgatatt tcacctgctc gccggtaaca tacagcgtat cgcctttcgc ctggatctgc
    12601 ggatcggcaa gtccggcatt gtatttcagc aacgttaact gattcgccac caccgagtgc
    12661 tgcaaaatgg catcctgcgg ctgcggttga tattgcgggc gggcgttatc gatgtaggac
    12721 gggcgcagat cgttaaagtt ggtgcgcagc gtgacgccaa acataaaggt gttgccacgt
    12781 tcatagctaa ggttaacgtc ggcccaatcg gtaacgcgat aaatcgcccc gacgttaaac
    12841 ttactttttt gctccagctt cccggcaaaa tcctgctgat aattattacc ttcatactcc
    12901 agtttcaggc gcagcggttg ccagggcgtc tggtattcca cgccgccaaa cagtgaggcc
    12961 gggccgtgga acatctgact gccgtcgata gatcccgctt gtttgtagct gttatcgcga
    13021 tagcaatatt tatcactggc tgaacagagc ggatttttca cattaccgct ggtgcccaga
    13081 tatccccagc ccaggccgag cgtaaaatcg aacggccccc aggctttgct ggcaacaaga
    13141 tattccgcat caaacagccc cgtaccgccg atatcccgcg caccaaccgc cacttgcggc
    13201 agccagtaac tctcttccca caaacgcagt ttgagatcga aggctttatc tttatacgtt
    13261 tgatcgccag agaacgcttc gacgctgctg tactgccggg tgcgcacgtc ggtgtaacgc
    13321 agcgttgttt ccagccacgg gaagagttgc actgaagctg agtaataacg gtactgatcg
    13381 ttatcgcgat agttcagact caactcccct tcccgtgcca tacgcgcggt gggagtttgt
    13441 aataatccta cgccaccgaa atccgactgc gacggaccaa tgggcgcagg atatgtttcg
    13501 ccgtagcaag ctgtgctaat gcccagcgcc agtaagctaa gcagatgtct ttttttcatt
    13561 attggggtat ccgctgcgtc agagtctgaa gaatgtcggc gttaagggca tcaggcgtct
    13621 cactccagac ggagtctgcg aggccaacat aaataatgct gccgggcatc ggctctacgt
    13681 gacgcttgtt ccagtaagcc accggcgctt tttgcgtgcg tccgtccggg taaacgaccc
    13741 acgcgtagct gcgatccgca ccgctgagca ggctctggtc agagagatag ctcgccacgt
    13801 cgcgacctgg agtgaatggc tgattgccag gatggctgat aagcccgaac aacgtgaccg
    13861 tggacggcgg tggtccgacc cacagcgtgt agttgccctg caacggcggg ttaccgcgtt
    13921 cggcaacgcg gacgatatcg ggatcaagat tgattttttg cctgcctgtc acttccaatg
    13981 cctggatttg ctggcgtaag gcgttaatgg cggcggcatc gtcggtgctg gaatctgcgc
    14041 cctgttctgc cagccgtgtc agcaacgcct gctgctgacg caatgctgcc gccgttgcca
    14101 gctcttcgct aatcaccgca ccaggccacc agctgtttgc cagtcgaggt tgccctacca
    14161 gatcgattaa atgctctgcg cccgttagcg ttttggcctc actgctgcca ttgctgaaca
    14221 ccttgacggt tcctgccgcg aagaccgatg acgctcccac actcaaaagc aacgcgacaa
    14281 tagtttgttt aatcattgtt ttgccgcctt gatgagagtg gttttcaccg ggaaataatc
    14341 cgcgccgaga tactgttccg actggcgaat ttgcccttcg ctgtcgatcc agtagcggtt
    14401 atgccagcgc gcctggtcgg tggagacttc ttcgtccagc acgcgaacca gggtttcatc
    14461 gctgccgacc ttgacggtat cggtgccatc ccatttgaag actgagcgtg cggtggcgta
    14521 gcgtacctgc tggtactcgg tccagcccat cgtgcgcgtc catgttgcac catcaacaat
    14581 ttgtgcgggt ttaatcagcg ggtcggcggc aaggttattc acttcaatca ggttgtcgcc
    14641 gccaagcaaa gtcttcacca gacgaccatg ttgtgtgaca agagtggctt gatcctgagt
    14701 gacccatttt tgttgtccgt cttcagcgaa ggccagcacc acaaacaatt gcggcccgcc
    14761 attaagctgc atgtactggc tggcgtaggg catattttga atatcatcgt ccgtcagctg
    14821 tacgcctggc gtgccgaaca gactgtccca cagtgaattg cccagctctt tagtcgtggc
    14881 tgaacaggcc tgtaataaca ggcagataag aatgagtgca ggtcgcttca cgactcttct
    14941 ccagaggggc agaataacca caccggagtg tggttatggt taatcccttc atatttcaag
    15001 ccacagatgc aatgactcga attattttgg ggatacatac tttattactg ggtactggtg
    15061 gttgtggttg ttgtggtccc ggtattagaa ccatcaccgc caccggttgc cgcgagcgcg
    15121 acacccaccg ccgagcttac ggtgctgacg ctggtcgcgg aggaactccc cgccgaaacc
    15181 gacgttgctg ccgcccctgc cgcttcgccc acctgtacag gtgcagccca cacagaagtc
    15241 gccgcaagcg cagatatggc aaaaatgcca tacagaactt ttttcataac aatttccctt
    15301 cattgaatga atggagattt acccaaaaac atttcgggcg ggatcgagta tacacaacta
    15361 aagcatgcga attacggcgc ggggaattag cgatggtgtt ttaggataat tggaatgaat
    15421 atcaaatata agtttcaact tatatttaaa taacaataaa aatcataaac ttaaaataaa
    15481 aacaaaagta attaatctgc gtattctccg aaataaacaa cgattaaaaa tctattttag
    15541 gattaacctc acggtatgat ttccgttaaa tcacagacaa ggggatttat ctgataaaac
    15601 aatgccgact tattgtcggc attgttttta aggttattta actcctcata ctatgcatta
    15661 atgtcgatat tggcatcagg ctccggttgc cggatgcggc gtaaacgcct tatccggcct
    15721 acatatcggc atcaggcacg catgatgatt aaccgcgcca cgctttatag cggttaatca
    15781 gaccattggt cgagctatcg tggctgctga tttctttgtc atctttcagc tctggcagaa
    15841 tacggttcgc cagttgttta cccaactcca cgccccactg gtcgaaggta aagatgttca
    15901 ggatcacgcc ctgagtaaag attttgtgct catacagcgc aatcaacgca cccaggctga
    15961 acggagtgat ttcgcgcagc aggatggagt tggtcgggcg gttaccttcg aacactttga
    16021 acggcaccac gtagtcaagc gttgccggat ctttaccctg atcgcgatat tcctgctcaa
    16081 ccacttcgcg ggatttacca aacgccagcg cttcggtctg ggcgaagaag ttagacagca
    16141 gtttctggtg gtgatcagag agcgggttat gggtgatagc cggggcgatg aaatcgcacg
    16201 gtaccatttt ggttccctgg tggatcagct ggtagaacgc gtgctgaccg ttagtgcctg
    16261 gttcacccca gataatcggg ccagtctggt aatccacaac gttaccgtta cggtcaacat
    16321 acttaccgtt ggactccata ttgccctgct ggaagtacgc cgcgaaacgg tgcatatact
    16381 ggtcatacgg cagaatcgct tcagtttccg caccaaagaa attattgtac cagatgccaa
    16441 tcagcgccag caggacaggc aggtttttct cggcaggcgt ggtggagaaa tgcttgtcca
    16501 tcgcgtgtgc gccagaaagc agttcaacga agttatcaaa gccgatggag agaacaatcg
    16561 acaggccaat cgctgaccac aaagagtaac ggccgccgac ccagtcccag aactcgaaca
    16621 tgttggcagt atcaatacca aactcgccaa cggctttggc attggtggaa agcgccgcaa
    16681 agtgttttgc aacgtgtttt tcatcacctg ccgctttcag gaaccagtca cgcgcgctat
    16741 gggcgttggt catagtttcc tgagtggtga aggttttaga tgctaccagg aacagcgtgg
    16801 tttccgggtt tacttttttc agcacttcag cgatgtgagt cccatcgacg ttagaaacaa
    16861 agtgcatgtt caggtgattt ttgtacggac gcagtgcttc ggtcaccatg tacgggccga
    16921 ggtcagaacc accgatcccg atgttcacta cgtcagtgat tgctttgccg gtataaccct
    16981 tccactcacc ggagataatc gcttccgaga aggttttcat cttctccagc accgcgttga
    17041 cttccggcat tacgtctttg ccatcaacca gaatcggggt attgctacgg ttacgcagcg
    17101 ctacgtgcag cacggcgcgg ttttcagtgc ggttgatctt ctcgccagag aacatcgact
    17161 taatcgcgcc cgccagatcg cactctttcg ccagatcctg taatttcgcc agcgtctctt
    17221 cagtgatgcg gtttttggag taatccacca gcatctgatc gtcgaatgtt gcggagaact
    17281 tagaaaaacg atcgccgtct ttagcaaaaa gatcggcgat cgtaacgtct ttcatttcat
    17341 cgaagtgttt ctgtagtgcc tgccaggcag cggtctgcgt tggattgatg tttttcatta
    17401 gcaatactct tctgattttg agaattgtga ctttggaaga ttgtagcgcc agtcacagaa
    17461 aaatgtgatg gttttagtgc cgttagcgta atgttgagtg taaaccctta gcgcagtgaa
    17521 gcatttatta gctgaactac tgaccgccag gagtggatga aaaatccgca tgaccccgtc
    17581 gttgacaacc gccccgctca ccctttattt ataaatgtac tacctgcgct agcgcaggcc
    17641 agaagaggcg cgttgcccaa gtaacggtgt tggaggagcc agtcctgtga taacacctga
    17701 gggggtgcat cgccgaggtg attgaacggc tggccacgtt catcatcggc tacaggggct
    17761 gaatcccctg ggttgtcacc agaagcgttc gcagtcgggc gtttcgcaag tggtggagca
    17821 cttctgggtg aaaatagtag cgaagtatcg ctctgcgccc acccgtcttc cgctcttccc
    17881 ttgtgccaag gctgaaaatg gatcccctga cacgaggtag ttatgtctga aattgttgtc
    17941 tccaaatttg gcggtaccag cgtagctgat tttgacgcca tgaaccgcag cgctgatatt
    18001 gtgctttctg atgccaacgt gcgtttagta gtcctctcgg cttctgctgg tatcactaat
    18061 ctgctggtcg ctttagcgga agggctggaa cctggcgagc gattcgaaaa actcgacgct
    18121 attcgcaaca tccagtttgc cattctggaa cgtctgcgtt acccgaacgt tatccgtgaa
    18181 gagattgaac gtctgctgga gaacattact gttctggcag aagcggcggc gctggcaacg
    18241 tctccggcgc tgacagatga gctggtcagc cacggcgagc tgatgtcgac cctgctgttt
    18301 gtcgaaatcc tgcgcgaacg cgatgttcag gcacagtggt ttgatgtacg taaagtgatg
    18361 cgtaccaacg accgatttgg tcgtgcagag ccagatgtag ccgcgctggc ggaactggcc
    18421 gcgctgcagc tgctcccacg cctcaatgaa ggcttagtga tcacccaggg atttatcggt
    18481 agcgaaaata aaggtcgtac aacgacgctt ggccgtggag gcagcgatta tacggcagcc
    18541 ttgctggcgg aggctttaca cgcatctcgt gttgatatct ggaccgacgt cccgggcatc
    18601 tacaccaccg atccacgcgt agtttccgca gcaaaacgca ttgatgaaat cgcgtttgcc
    18661 gaagcggcag agatggcaac ttttggtgca aaagtactgc atccggcaac gttgctaccc
    18721 gcagtacgca gcgatatccc ggtctttgtc ggctccagca aagacccacg cgcaggtggt
    18781 acgctggtgt gcaataaaac tgaaaatccg ccgctgttcc gcgcgctggc gcttcgtcgc
    18841 aatcagactc tgctcacttt gcacagcctg aatatgctgc attctcgcgg tttcctcgcg
    18901 gaagttttcg gcatcctcgc gcggcataat atttcggtag acttaatcac cacgtcagaa
    18961 gtgagcgtgg cattaaccct tgataccacc ggttcaacct ccactggcga tacgttgctg
    19021 acgcaatctc tgctgatgga gctttccgca ctgtgccggg tggaagtaga agaaggtctg
    19081 gcgctggtcg cgttgattgg caatgacctg tcaaaagcct gcggcgttgg caaagaggta
    19141 ttcggcgtac tggaaccgtt caacattcgc atgatttgtt acggcgcatc cagccataac
    19201 ctgtgcttcc tggtgcccgg cgaagatgcc gagcaggtgg tgcaaaaact gcatagtaat
    19261 ttgtttgagt aaatactgta tggcctggaa gctatatttc gggccgtatt gattttcttg
    19321 tcactatgct tatcaataaa cgagcctgta ctctgttaac caacgtcttt atcgagaata
    19381 attgccttta atttttttat ctgcatctct aattaattat cgaaagagat aaatagttaa
    19441 gagaaggcaa aatgaatatt atcagttctg ctcgcaaacg aattcccgta cactgaactc
    19501 tctggctcac tgagggcacc atcatggcac tcccccgtat tacccaaaaa gagatgactg
    19561 aacgcgagca gcgtgaactt aaaacactgc tggatcgggc gcggattgcg catggtcgcg
    19621 tactgaccaa ctccgaaacc aacagcatta agaaagagta catcgataaa ttaatggtcg
    19681 agcgtgaggc tgaagcgaaa aaagcccgcc aattgaagaa aaagcaggct tataaaccgg
    19741 atcctgaagc gtcatttagc tggtcagcaa atacgtcgac gcgcggcagg cgctaatgaa
    19801 caatcaacgc ctgagatttt cactcgtctg tgtgaacaca attttcccct caccctaacc
    19861 cttttcccga aggggcgagg ggactgtccg agcacttttg ggccgttttc atcagtctga
    19921 ggcgttaatc agcgcccctt ctttttacgc cccggcgagg taaaacgctt accgttggac
    19981 gccgggcgac cgcctttttc cgccgttttt tccatcttaa cgactggacg tttgatgccc
    20041 gctgttttcg gtttggcctt cgctttgggc ttcgcctcgg aagaggaatt ttcaatgagc
    20101 ttaaagagat cgataagctc gtcgtcggtt aaatcgcgcc attctcccag cggaatgccg
    20161 cttaagctga cgttcataat gcgcgtacgt tccagctttt tcacttcata gccgaaatgc
    20221 tcgcacatgc gccggatctg acggttcaac ccctgcacca gggtaatgcg gaagacaaac
    20281 ggtgcttctt ttttgacttt gcactttttc gtcaccgtcc cgaggatcgg cactcccgcc
    20341 cccataccac gaataaattc atcggtaatc ggtttatcga ccgtcaccag atactctttc
    20401 tcatgatcgt tacccgcacg gaggatctta ttcaccagat cgccgtgatt ggtgaggaaa
    20461 atcagcccct gggagtcttt atccaggcgg ccaatcggga acacgcgttt gctgtggtta
    20521 acgaaatcga caatgttatc gcgctcgcca tcttcggtgg tgctgacaat accgacgggc
    20581 ttgttcaggg cgataagtac caaatcttcg gcttcccgag gctcaatcaa ctgaccgttt
    20641 actttcacaa tgtcgccggg tttcacctga tcaccaatgg tggctcgctt gccattaagg
    20701 aacacattgc cttgctcgat atagcgatcc gcttcgcggc gtgagcaaat tccgctttcg
    20761 ctgatgtatt tatttaaacg gactgatgag tcgggcagca taaattctcc tgtaaaacgc
    20821 ggaatatacc gtacctctta gcggggaaaa acagggttat gcggcaacgt cgaaattaaa
    20881 tgtgatctgc cacgcctttt atcacaaaag tgtctccgga agtacgatgc aaatttgcct
    20941 ggcaaaataa ggataatcaa catattacga atcgctgcac acctgtgctg atgactacta
    21001 cgcagagaaa caaatgtgca agagaaagcg ctatttctcc ccatgcaaag agtgaaaaat
    21061 cgctttgaaa ggccctcata atggggttat atcagatcaa gcggtgagaa agcgtatttg
    21121 cacaaatgtg cagaaccagg aggcggcaat ggaaaacagt gacgatatcc gtttgattgt
    21181 gaagattgcc caactctatt acgaacagga tatgacgcag gcgcaaatcg cgcgcgaact
    21241 gggtatttac cgcaccacca tcagccgctt gcttaaacga ggccgcgatc agggaattgt
    21301 caccatcgcc atcaactatg actacaacga aaatctctgg ctggagcagc aactgaagca
    21361 aaagtttggc ttgaaagacg ttgtggtggt gtcgggaaat gatgaggatg aagagactca
    21421 actggcgatg atggggttac acggcgcgca actgctggat cgcttgctgg agcctggcga
    21481 tattgtcggt ttttcctagg gtcgcgcggt gagcgcactg gttgaaaact tgccgcaagc
    21541 ggggcaatcg cggcagttaa tctgcgtgcc aattattggc ggaccgtccg gtaaactcga
    21601 aagccgctat cacgtaaaca cattaaccta cagcgtggca gcgaagctga aaggggaatc
    21661 gcatctcgcg gattttccgg ctctgctgga taacccctta attcgtaatg ggatcatgca
    21721 gtctcagcac tttaaaacca tctctgccta ctgggataat ctggatgtcg ccctggtggg
    21781 aattggctca ccggccattc gcgacggcgc taactggcat gcgttttatg gtggtgaaga
    21841 gagtgacgac ctgaaagcag gcgcggtatt ccattggcat tgccatgagt gaagaaaaat
    21901 acagcggaat tgttggtgca ctgcgtggaa aatatattaa ttgtctggta acgaatagca
    21961 gcacagctga actattactg aaataaaaat cttcccgatg tttaccgtaa taaaaattac
    22021 ggcgggtccc tattatctaa aatcaggagt ctgttatgca aacgtggtta aatttgcagg
    22081 ataaaatcat tatcgtcacc ggcggcgcat ccggtattgg tctggcgatt gtagaggaat
    22141 tattagcaca aggcgcaaac gtacagatgg tagatattca cggtggcgat ggtcaatatg
    22201 aagttcataa aggctatcag ttttggccga ccgatatttc cagcgccaaa gaggtaaatc
    22261 atacggtagc ggaaattatc cagcgttttg gtcgcatcga cggtctggtc aataacgccg
    22321 gggtcaattt cccgcgtctg ctggtcgatg agaaagcgcc tgccgggcag tatgaactca
    22381 acgaagctgc attcgaaaaa atggtcaata tcaaccaaaa aggcgttttt ctgatgtcgc
    22441 aggcggtggc gcgccagatg gtcaaacaac atgatggcgt gattgtgaat gtttcctcag
    22501 aaagtggact ggaaggctca gaaggccaaa gctgttacgc cgctaccaaa gccgcgctca
    22561 atagcttcac gcgctcctgg tcgaaagagc tgggtaaaca cggtatccgt gtggtcggta
    22621 tcgcgccggg gattctggaa aaaaccgggc tacgcacgcc ggaatatgaa gaggcgctgg
    22681 cgtggacgcg caatatcacc gtcgagcagc tgcgtgaagg ctataccaaa aacgccattc
    22741 ctattgggcg cgccggaaga ttagcagaag tcgctgattt tgtttgttat ctgctgtctg
    22801 aacgcgccag ctacatcacc ggagtaacca ctaacattgc gggcggcaaa acgcgcggat
    22861 aaggaggctt tatggtcaac gccatttttt gtgcccacgg caaattagct tgcgccatgc
    22921 tggaatcggt tcagatggtg tacggcgacg ccaatgtcga ggcggttgaa tttgtaccag
    22981 gcgagaacgc cggagacatc gtagcaaagc tggaaaagtt agtaagcatt cacaatcaag
    23041 atgagtggct gattgccgtg gatttacagt gcggcagccc gtggaatgcg gcagcgatgc
    23101 ttgcgatgcg caatcctcgt ctacgggtaa tcagcggcct ttccctgccg ctggcgctcg
    23161 aactggttga taaccaggac agcatgaatg ttgatgaact gtgtgaacac ctgacgcaaa
    23221 tcgcaaagca aacctgcgtt gtctggaaac aactggcaac ggcagaggaa gatttctgat
    23281 gaacattacg ctcgcccgca ttgatgaccg tctgatccac ggccaggtca ctaccgtctg
    23341 gtcgaaagtg gctaacgccc agcgcattat tatctgcaat gacgaagttt ataacgatga
    23401 agtgcgccgg acattattgc gtcaggctgc gccaccggga atgaaggtta acgtggtcaa
    23461 tattgaaaaa gccgtcgcgg tttatcacaa cccgcaatat taggacgaaa ccgtttttta
    23521 tcttttcact cgcccacagg atgccttagc gatggttcgc cagggggtaa aaataggcac
    23581 cctgaatatt ggcggcatgg cctggcggcc cggtaaaaaa cagttaacca aagcggtttc
    23641 attagatgat gatgatatta acgcatttca tgagttaaat aatcttggcg tcataatgga
    23701 tttacgcgtc gtagcgtcag atccctcaat caatattatc gacaaaataa acgaacagtt
    23761 aatcgcaaat taaataatag cgcctgtgta catatgcttt aacaggcagg gatgacgaca
    23821 cttaaaaggt gacaaatcat ggaaataagt accctacaaa ttattgctat atttcttttt
    23881 tcctgtattg ccggaatggg cagcgtgctg gatgaatttc agactcatcg cccgttaatt
    23941 gcctgtacgg tgattggttt aattctcggt gatttaaaaa ccggaataat actcggtggt
    24001 acgctggaat tgatagctct cggctggatg aacgtcggcg cggcgcaatc tccggattct
    24061 gcactcgcca gcataatctc cgccattctg gttatcgttg gtcagcaaag catcgccacc
    24121 ggaatcgcca tcgcgttgcc tgtggctgcg gcaggccaag tgctgacggt gtttgcccgt
    24181 accatcaccg tagtgttcca gcacgcggcg gataaagcag cggaagaggc gcggtttcgc
    24241 actctcgata ttctgcatgt ctccgcgctt ggcgtgcagg cgctgcgcgt tgctattccg
    24301 gcactgattg tctcactgtt cgtcagcgcc gatatggtca gcaatatgtt aaacgccatt
    24361 cccgaatttg tgacccgtgg actgcaaatt gctggcggtt ttatcgtggt ggtcggttac
    24421 gccatggtgc tacgtatgat gggcgtgaaa tatttgatgc ctttcttttt cctcggtttc
    24481 ctcgcaggtg gctacctcga tctcagcctg ctggcgttcg gtggcgtcgg cgtgatcatg
    24541 gccctggtct acatccagct aaatccacag tggcgtaaag ctgaacctca actccaggcc
    24601 accgcctcca ccgcccttga ccaacttgac gattaacgga gccatcatgg aacagagaaa
    24661 aattacacgc agcgatctgg tgagcatgtt tctgcgctcc aacctgcaac aggcgtcctt
    24721 taactttgaa cgtattcacg gactgggctt ttgctacgac atgatccccg ccatcaagcg
    24781 actttaccca ttaaaagagg atcaggttgc ggcgctcagg cgacacctgg tgttcttcaa
    24841 taccacgcca gccgtatgtg gcccggtcat cggcgtcact gccgccatgg aagaggcgcg
    24901 ggccaacggc gcggaaattg atgacggtac cattaacggc atcaaagtcg gtctgatggg
    24961 accattggca ggagttggcg atccactggt ctggggaacg ctgcgcccga ttaccgctgc
    25021 gctcggcgca tctctggcac tttctggcaa catcctcggc ccgctgctgt ttttctttat
    25081 tttcaatgcg gtgcgtctgg cgatgaagtg gtatggccta cagctcggct ttcgcaaagg
    25141 ggtgaatatc gtcagcgata tgggcgggaa tgtgctgcaa aaactcaccg aaggcgcgtc
    25201 gattctcgga ctgtttgtga tgggcgtgct ggtcaccaaa tggacgtcaa tcaacgtacc
    25261 gttggtggtt tcacaaacgc atgccgccga tggctccacc gtcatcatga ccgtgcagaa
    25321 cattctcgac caactttgcc ctggtttgct ggcgctcggt ctgacgctac tgatggttcg
    25381 tctgctcaac aaaaaaatta acccggtatg gctgattttc gccctgtttg gcttagggat
    25441 tatcggcaat gcgctgggct tcctgtcctg attcttcgcc ccggcacgac tgccggggcc
    25501 atcgctcaac atgaggtggt ttatgaaaac aacagctctg cgtctttatg gtaaacgtga
    25561 tttacgcctg gaaacctttg accttcctga aatgcaggag gatgaaatcc tcgcgacggt
    25621 ggtcactgac agcctgtgcc tctcttcctg gaaagaggca acctgggtga aaaccataaa
    25681 aaagtacccg acgatgtggc gaccaaccca atcatcatcg gccacgagtt ttgcggcgat
    25741 attctggccg tgggtaaaaa gtggcagcac aaattccagc cgggtcagcg ttatgtgatt
    25801 caggccaacc tgaaactccc tgaccgcccg gactgccccg gctactcctt cccgtgggtg
    25861 ggcggcgaag ccacgcatgt ggttattccc aacgaggtca tggaacaaga ttgcctgctg
    25921 gcatacgacg gcgaaaccta ttttgaaggc tcgctggttg aaccgctttc ctgcgtgatt
    25981 ggcgcgttca acgccaacta tcatcttcag gaaggtagtt ataaccacac gatggggatt
    26041 cgcccgcaag ggcgcacgct gatcctcggc ggcaccggac caatgggatt gttggcgatt
    26101 gattatgcgc tgcatggacc agttaacccg tcgctgctcg tcattaccga taccgacaac
    26161 gacaaattga gttatgcgcg caagcactat ccatcagaac cgcaaacact gattcattat
    26221 ctcaatgcca ccgatgcagc atttgatacg ttaatggcgc tgagtggcgg tcacggcttc
    26281 gatgatattt tcgtctttgt gcctaatgaa ggactggtga ctctcgcctc ttccttgctg
    26341 gcgacagatg gttgcctgaa tttcttcgcc ggaccgcagg ataaacattt cagcgcgcca
    26401 attaatttct acgatgtgca ttatgcattt acccactacg tgggcacgtc aggcggcaat
    26461 accgacgaca tgcgcgcggc ggtcaaactg attgaagaga aaaaagtgca ggccgcaaaa
    26521 gtggtaacac atattcttgg gctgaatgcc gcgggcgaaa ccacgcttga attgcctgcc
    26581 gtcggcggcg gcaaaaagct ggtgtatacc gggaaatacc tgccgctgac gtcactcacg
    26641 cagattcagg atcaagcact ggcggcgatt ctggcgcgtc atcaggggat ctggtccggt
    26701 gaggcggagc aatatctgct cactcatgca gaggcaattt cccatgatta atcgcgctac
    26761 ttaactctgc atgtcgctgg ctggtcgccg cggcaacttc gcgacctgat gtgcggctga
    26821 ttgctgaagc ggcccaatgt gcacgagaag gataaaaaaa catcagcgcc ttaaggggcg
    26881 ctcaaattgg tggcaaagta cacattgttt atgccggatg cggaataaag ccttattcgg
    26941 cctacataat ctcgaaaatt caatatattg cagttatctg gtaggcctgg taagcgtagc
    27001 gcatcaggca atgctgtgtt tgtcatcagt ctcagcgccc ggtgcaatac gtgacatcgt
    27061 gagccatgtc agtaagtggt taaattcgca gtggtagcat gccttccgtg atttcttact
    27121 aaccggagaa gtaatggaac tgcttttatt gagtaactcg acgctgccgg gtaaagcctg
    27181 gctggaacat gcactgccgc taattgctga acagttgcag ggtcgccgct cagcggtgtt
    27241 tatccctttc gctggcgtaa cgcagacctg tgatgattac acagcgaaaa cggctgcggt
    27301 tctcgctccg ctgggtgttt ctgtcaccgg tattcatagc gttgtcgatc ccgttgccgc
    27361 gattgaaaat gctgagatcg tgattgtcgg gggcgggaat actttccagt tgctgaaaca
    27421 gtgccgcgag cgcgggctgc tggcaccaat tactgacgtg gttaaacgtg gtgcgctgta
    27481 tattggctgg agcgcaggcg ctaaccttgc ttgcccaacc attcgtacca ccaacgatat
    27541 gccgattgtc gatccgcaag gtttcgatgc gctaaatctg ttcccgctgc aaatcaaccc
    27601 gcacttcacc aacgcgctgc cggaaggcca taaaggtgaa acccgtgagc agcgtattcg
    27661 cgaactgctg gtcgtcgcgc cagaactgac gattattggt ctaccggaag gtaactggat
    27721 cacggtgagt aaaggtcacg ctacgctggg tggcccgaac accacttatg tgtttaaggc
    27781 cggtgaagaa gcggtgccgc tggaagcggg tcaccgtttt taagtgaatt gccggatgca
    27841 tgatccggca tcttgttcat agtgcctgat gcgacgcttg acgcgtctta tcaggcctac
    27901 aggattcaca atattaatac tcgtcccgtc cttcatcttc atccggctgt tccagcacac
    27961 tgtaagccac cgaacaaaac agcgagttca gacgctgcat atctcccagt aagcctaaat
    28021 gtagcgaact ggtttcaatg ctctgcacgt tttgctgatg caggcgatcg acgtgggcgt
    28081 gggaatagcg gcgattaaga atgcgaaagc gatgcttgct gcggcgcaaa cgacgagcgc
    28141 tggtgacatc gccagagaag aacaccgaca ttgccagctt taaattactg agcaattgct
    28201 catagagcgc atccagttcc ttcaacccat caagcgaaaa tgcccgccgt gctgccagcg
    28261 atttatcagc aatttcgctg cccatgcgct cgacgatatc ggaagcctgt tcaaggttga
    28321 gcgacatttc aataatctcc gcccagcgac gcgactcttc ttctgccagt tcctcttttg
    28381 gcattcgcgc cagatacagc ttaatggcgg tgtagagaac gttgatatca tctgccagct
    28441 tacgcagctc tttctcctgc cgtggctcgc cgtgcatcac tttattcagc ccttccatca
    28501 tctgttccat ggcgtcgcca atgcgcaggg tttcgcgcgc ggcgttcgcc agagcaagcg
    28561 tgggcgtatc cagcgcgctg acatccagat gtttaggccg tagctgggta tccagttccg
    28621 gttcatcgcg aataatcgtt ttgcaaaacc gtgccatcgg gtcaacaaat ggcagcatga
    28681 ccaggcaacg tacaaggttg tagaagacgt ggaaatagat caccagttcc gcttttggca
    28741 atgacaactt ccccattgtc tctgccagca aatggacaaa cggcaggata atcaggctac
    28801 ccaccagctt aaacagcaaa ctacccagcg cgacacggcg ggctgcggca ttggcggcac
    28861 tgttgttgag catcgccagc aggccggaac cgaggttagc accaatcacc agacagagcg
    28921 ccacggggaa ggagataatg cctgcggcgg tcagagtcgc ggtcagcagt acagcagcaa
    28981 ggctggagta gctgataatg gcgaacatcg cgccaatcag cgcatccagc agaatatcgc
    29041 cggtcagcga ggcaaagatc acctgaacgc cgtttgcctg ggtgatcggc gttacggcct
    29101 gcacaatcaa ctccagcgcc agcaaaatca gcccaagacc aataccgacg cggcccagtt
    29161 gcccggcgcg tgactgtttg cgtccgagga aaaaaatcac gccgataaaa ataagtaacg
    29221 gtgacagcca ggataagtcg aaggtgagaa tacgcgccat tagcgccgtc ccgacatctg
    29281 cacccagcac aatcaccaga gccggtgcga gggctaccag atcctgagcg acgaacgagg
    29341 tcaccagcat ggtggtggca ttactgctct gtaccagtgc ggtaacgccg atccccgcgc
    29401 aaaaggcgag cggctttttt tcgacgctcc ggctaaggac agtacgcaaa cgcgcgccga
    29461 agacgcgcat tacgccggtt cgaacaatat gagtccccca gaccagcagg gcgacggcag
    29521 aaagcaggtg aagcagcgtt aacacgagtt caggttctcc ttatcgttat atgttcctga
    29581 agcaattgaa tgaccaaagg accggaaaca cgctgactgg catctttact gtgccttttg
    29641 acattcgttt ttccggctaa gattattcca gcctgtttcc agtataagtg tttcatatta
    29701 ataaagagac agggcactca ttgagcgccc tgtttgttgt aaggaaaagt gacagatttg
    29761 tgaatcagtc cgcgtcatac cccagattcg gtgccagcca gcgctcaaca tcggagacgc
    29821 tcataccttt acggcgggca tagtcttcaa cctgatcgcg ctgaatttgc gccaccgcgt
    29881 agtacttgct gtccgggtgg ctgaaatacc aacccgaaac cgatgcacca ggccacatgg
    29941 cgaaagattc tgtgagtttc atgccagtat gtttttccac ttccagcagc tcccagatgg
    30001 tggctttttc cgtatgttcc gggcaggccg ggtagcccgg tgccggacgg atgccctggt
    30061 agttttcgcg gatcagttct tcgttgctga ggttctcgtt cggcgcatag ccccagtaga
    30121 ctttacgcac acgctcatgg agatactccg caaaggcttc ggctaaacgg tcggcaagcg
    30181 ctttcaccat gattttgttg taatcatcgt gctgcgcttc aaaggcatca gccagtgcgt
    30241 cctcttccag cccgccagtc acggcaaatg cgccgatgta atctgcttta ccagaaagct
    30301 tcggcgcaac gaagtcagcg agacagtagt tagcgaatcc cgttttttcg gtctgttgac
    30361 gcagatggtg gctgacgttg atcacatggg tgcgcgtttc gtcacggtag atttcaatgt
    30421 catcgcccac acggtttgcc gggaacaagc ccaccacgcc acgcggattc agcgttttct
    30481 cggcgcttaa tttatccagc atgtcgttgg cgtctttaaa cagccgctgc gcctcaacgc
    30541 ccaccacttc atcttccaga atgcgcggat acttcccggc cagcgaccag gtcataaaga
    30601 acggtgtcca gtcgatgtaa ttacgcagag tttcgatgct ggcttcaact tcctgcacgc
    30661 cgagacggtg cgccaccggc ggcgtgtaag cctgccagtc aaaagcgaag tcgttatcgc
    30721 gcgccgcttc cagcgtgacc ggtggtgtgc gcggtttctt gcgcccgtgc tgaatacgta
    30781 cggtttcata ctccttgcgg gtacgagcga caaaatcatc gcgctgagta tcggaaagca
    30841 gcgccgccac cacaccaacg gtgcgcgagg cattctgcac atacaccgtc gggccgctgt
    30901 agttctgctc aattttcacc gccgtgtgcg cttttgaggt cgtcgcgcca ccgatcagta
    30961 acggaatggt gaagccctga cgctccatct ctttcgccac gttaaccatc tcgtccagcg
    31021 acggcgtgat aagccccgaa agtccaatca gatcagcatt cacttcttta gcggtacgga
    31081 gaattttttc cgcaggcacc ataacgccga gatcgacaat ttcgtagttg ttacattgca
    31141 gcaccacacc aacgatattt ttaccgatgt cgtggacgtc gcccttcacg gtggcgatca
    31201 ccatcttgcc gttggttttg ccctgctctt tgctggcttc aataaacggt tcgaggtagg
    31261 ccactgcctg tttcatgacg cgcgccgatt tgaccacctg tggcaggaac attttcccct
    31321 cgccaaacag atcgccgacc acgttcatgc cgtccatcaa cgggccttca atcacttcaa
    31381 tcgggcgcgt agcctgctgg cgggcttctt cggtatcctg ctcgataaac tcagtaatgc
    31441 ctttgaccag cgagtattcc agacgtttat tcacttccca cgagcgccac tccgcctgct
    31501 gggcgttagc ggtgtcgtcg gttttgctgc cgcgatattt ctcggcaagc tccagtaaac
    31561 gttcagtgcc atcatcgcga cgattaagaa tcacatcttc caccgcgtcg cgcagttcag
    31621 cgggcaggtc gtcgtaaatc gccagttgcc cggcgttgac gatccccata tccatgccat
    31681 tgcgaatagc gtagtagagg aacaccgcgt gaatggcttc gcgcaccggt tcgttgccac
    31741 ggaatgagaa agaaacgtta gatacgccgc cggaaatcag cgcgtgcggc agttcgcgtt
    31801 tgatgtcttc gcaggcgccg ataaagtcct gcgcatagtt gttgtgctct tcaatgccgg
    31861 tggcgaccgc gaagatgttc gggtcgaaga tgatatcttc tggcgggaag ccaacctctt
    31921 cggtgaggat tttgtacgcc cgacggcaaa tctcgatttt ccgtgcgcgg gtatcggcct
    31981 gtccctgttc gtcaaaggcc attaccacca ccgctgcacc gtaacgacgc aacagtttcg
    32041 catgatggat aaaggcgtcg acgccctctt tcattgagat agagttaaca atgcctttgc
    32101 cctgaataca cttcagcccc ttttcgatga cctcccattt tgaggagtcg atcataatcg
    32161 gcacgcgagc gatatccggt tcaccggcaa tcagattgag aaaacgcacc atcgccgctt
    32221 cggcgtcgag catcccttca tccatattga tatcgataat ctgcgcgccg ttttccacct
    32281 gctggcgggc cacatccagt gcctcgctgt atttctcttc tttgatcagg cgcttgaact
    32341 tagcggaacc ggtgacgttg gtgcgttcac ccacgttcac aaacaggcta tcttcgccaa
    32401 tgttcagcgg ctccaggccg gacaaacggc aggctacggg gatttccggc agtttgcgcg
    32461 gcgctaatcc ttctactgca cgactcatcg ctgcaatatg ttgtggcgtg gtgccacagc
    32521 agccgccgac gatattgaga aagcccgctt ccgcccattc acgtatttgt tttgccatcg
    32581 tgtcggcgtc gagatcgtac tcaccaaagg cgttgggtag cccggcgttc gggtgcgcgg
    32641 tgacgtagca ttccgcaatc cgtgacagct cctgcacgta ctggcgcagt tcatcgggcc
    32701 ccagcgcaca gttcaggcca aaggtcagcg cttcggcgtg gcgcaatgag ttgtaaaatg
    32761 cttcggtggt ctgcccggag agcgtgcgcc cggaggcgtc ggtgatggtg ccagagatca
    32821 taatcggcag ctcaacgccc agcgtttcaa actccgtttt taccgcaaat accgccgctt
    32881 tggcgttaag ggtgtcgaaa acggtttcaa tcaggatcag atccgcacca ccttccacca
    32941 gcgctttggt ggactctcga taagccacca ccagcccgtc aaaagtgata ttacgaaatg
    33001 ccggatcgtt gacgtccgga gaaatagacg ccgtgcggtt ggttgggccg agaacaccgg
    33061 caacgtagcg cggtttctcc ggcgtgcgcg cggtccactc gtcagcacag gcgcgcgcca
    33121 gtttcgccgc cgcaaagttg atttccgccg acagggattc catctggtaa tccgccatcg
    33181 caatggtcgt ggagttgaag gtgttggttt cgatgatatc cgcgcccgct tcaaagtagg
    33241 cgttgtggat agcggcgatc acttccggtt tactgagtac cagcaggtcg ttgttgcctt
    33301 tgaggtcgca tggccagtcg gcaaagcgtt caccacgaaa atcggcttcg ttcagtcgat
    33361 agctctggat catggtgccc ataccgccgt ccagcaccag aatacgttca tttaactgcg
    33421 cacgcagttg ttccactttg ctgctcacac ttgctcccga cacgctcaac ccagatttaa
    33481 taaaaattca acaaaccata ctggcataaa cgcatctgtg gtaaaagcga ccaccacgca
    33541 acatgagatt tgttcaacat taactcatcg gatcagttca gcaactattg cattaactaa
    33601 caataaaaat gaaaatgatt tccacgatac agaaaaagga gactgtcatg gtcgcaccca
    33661 ttcccgcgaa acgcggcaga aaacccgccg ttgccaccgc accagcgact ggacaggttc
    33721 agtctttaac gcgtggcctg aaattactgg agtggattgc cgaatccaat ggcagtgtgg
    33781 cactcacgga actggcgcaa caagccgggt tacccaattc cacgacccac cgcctgctaa
    33841 ccacgatgca acagcagggt ttcgtgcgtc aggttggcga actgggacac tgggcaatcg
    33901 gcgcacatgc ctttatggtc ggcagcagct ttctccagag ccgtaatttg ttagcgattg
    33961 ttcaccctat cctgcgcaat ctaatggaag agtctggcga aacggtcaat atggcggtgc
    34021 ttgatcaaag cgatcacgaa gcgattatta tcgatcaggt acagtgtact cacctgatgc
    34081 gtatgtccgc acctatcggc ggtaaattgc cgatgcacgc ttccggtgca ggtaaagcct
    34141 ttttagccca actgagcgaa gaacaggtga cgaagctgct gcaccgcaaa gggttacatg
    34201 cctataccca cgcaacgctg gtgtctcctg tgcatttaaa agaagatctc gcccaaacgc
    34261 gcaaacgggg ctattcattt gacgatgagg aacatgcact ggggctacgt tgccttgcag
    34321 cgtgtatttt cgatgagcac cgtgaaccgt ttgccgcaat ttctatttcc ggaccgattt
    34381 cacgtattac cgatgaccgc gtgaccgagt ttggcgcgat ggtgattaaa gcggcgaagg
    34441 aagtgacgct ggcgtacggt ggaatgcgct gactttttct ggcgggcaga ggcaatattc
    34501 tgcccatcat acctgagtgg caatagaata agggtgtctg ttaatcgcat tgacgccaaa
    34561 ataacttaat gtcatacact tcacacactt ttattaactc agcattattt ttaaacatca
    34621 aaccacttaa ttattaacaa aatcgcaaga tctcattcca ccgaaacttt tacggctgca
    34681 ttatgagatt gagcgattta tgatatttct ttagttgtaa tcatattaaa ttttttcact
    34741 gttctgatag ttaaaattta agacatcaat aaacaatgag atatttaaat gattactcgt
    34801 attcctcgta gttctttctc tgcaaatatt aataatacag cccagacaaa tgaacaccaa
    34861 accctgagtg aattgtttta taaagaactc gaggataaat tttctggcaa ggagctggcg
    34921 acgcctctat taaaaagctt ctcagagaac tgtagacaaa atggtcgtca tatctttagc
    34981 aacaaggatt ttgtcattaa attttccacg tccgtcttac aagctgataa gaaagaaatt
    35041 acgataatta ataaaacgaa aacacgacac tcactcaaac cattgctcca atatttgaag
    35101 aatacctaat ggaaatttta cctcaacgct cagacactct tgataaacaa gaattaaacc
    35161 taaaatcaga tagaaaagaa aaagaattcc caagaattaa acttaatggt caatgttatt
    35221 ttccggggcg accccaaaac cgtatagtat gccgacacat tgctgcacaa tatattaatg
    35281 atatttatca gaatgttgat tacaaacccc atcaagatga ttattcttca gctgaaaaat
    35341 ttctcactca cttcaacaaa aaatgcaaaa accagacttt ggcgttgatt tccagccgtc
    35401 ctgaggggcg ttgcgttgct gcctgcggtg atttcgggct agttatgaaa gcatattttg
    35461 acaagatgga atcaaatggc atcagtgtta tggcagccat attactggtg gataaccatg
    35521 ctttgacggt ccggctaaga ataaagaaca caactgaagg atgtacccat tacgtggttt
    35581 cggtttatga tcctaatgta actaacgaca aaataagaat catgagcgaa agcaaagagg
    35641 atattaaaca ctattctctg atggatttta tgaatgtaga ttatagcctc ctgaaatggt
    35701 caaatgatca tgttattaac caatctgttg caataattcc agcacttccg aaagaacagc
    35761 tattgatatt aaaagga
//
LOCUS       DAJTZL010000024        34981 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.024, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000024 DAJTZL010000000
VERSION     DAJTZL010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34981
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.024"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..815)
                     /locus_tag="OO544_001017"
     CDS             complement(<1..815)
                     /locus_tag="OO544_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR6766674.1"
                     /translation="MKPAHNPSFFRSFCGLGCISRLSVEEQNITDYHRIWDNWAKEGA
                     ATEDRTQAVRLLKICLAFQEPALNLSLLRLRSLPYLPPHIQELNISSNELRSLPELPP
                     SLTVLKASDNRLSRLPALPPHLVALDVSLNRVLTCLPSLPSSLQSLSALLNSLETLPD
                     LPPALQKLSVGNNQLTALPELPCELQELSAFDNRLQELPPLPQNLRLLNVGENQLHRL
                     PELPQRLQSLYIPNNQLNTLPDSIMNLHIYADVNIYNNPLSTRTLQALQRLTSS"
     gene            907..993
                     /locus_tag="OO544_001018"
                     /pseudo
     CDS             907..993
                     /locus_tag="OO544_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1397..1873)
                     /locus_tag="OO544_001019"
     CDS             complement(1397..1873)
                     /locus_tag="OO544_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HCR6766675.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDIERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            complement(1932..3221)
                     /gene="bioA"
                     /locus_tag="OO544_001020"
     CDS             complement(1932..3221)
                     /gene="bioA"
                     /locus_tag="OO544_001020"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HCR6766676.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVVSAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKSQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKICDREGILLIADEIATGFGRTGKLFACEHAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQQ
                     QVADIEVQLREQLAPARDAEMVADVRVLGAIGVVETTHPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            3308..4348
                     /gene="bioB"
                     /locus_tag="OO544_001021"
     CDS             3308..4348
                     /gene="bioB"
                     /locus_tag="OO544_001021"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HCR6766677.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            4345..5499
                     /gene="bioF"
                     /locus_tag="OO544_001022"
     CDS             4345..5499
                     /gene="bioF"
                     /locus_tag="OO544_001022"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HCR6766678.1"
                     /translation="MSWQEKINAALDARRTADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWQQGAEKFGVGSGGSGHVSGYSVVHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFTHNDVAHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            5486..6241
                     /gene="bioC"
                     /locus_tag="OO544_001023"
     CDS             5486..6241
                     /gene="bioC"
                     /locus_tag="OO544_001023"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HCR6766679.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHAELQRRSADVLLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLVQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDEIEQSLNGVHYQHHIQPITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            6234..6911
                     /gene="bioD"
                     /locus_tag="OO544_001024"
     CDS             6234..6911
                     /gene="bioD"
                     /locus_tag="OO544_001024"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HCR6766680.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            7490..9511
                     /gene="uvrB"
                     /locus_tag="OO544_001025"
     CDS             7490..9511
                     /gene="uvrB"
                     /locus_tag="OO544_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HCR6766681.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(9703..10611)
                     /gene="yvcK"
                     /locus_tag="OO544_001026"
     CDS             complement(9703..10611)
                     /gene="yvcK"
                     /locus_tag="OO544_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HCR6766682.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     regulatory      10876..11020
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            11008..11997
                     /gene="moaA"
                     /locus_tag="OO544_001027"
     CDS             11008..11997
                     /gene="moaA"
                     /locus_tag="OO544_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HCR6766683.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEVGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     gene            12019..12531
                     /gene="moaB"
                     /locus_tag="OO544_001028"
     CDS             12019..12531
                     /gene="moaB"
                     /locus_tag="OO544_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HCR6766684.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            12534..13019
                     /gene="moaC"
                     /locus_tag="OO544_001029"
     CDS             12534..13019
                     /gene="moaC"
                     /locus_tag="OO544_001029"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HCR6766685.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIVGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            13012..13257
                     /gene="moaD"
                     /locus_tag="OO544_001030"
     CDS             13012..13257
                     /gene="moaD"
                     /locus_tag="OO544_001030"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HCR6766686.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADYPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            13259..13711
                     /gene="moaE"
                     /locus_tag="OO544_001031"
     CDS             13259..13711
                     /gene="moaE"
                     /locus_tag="OO544_001031"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HCR6766687.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            13848..14552
                     /locus_tag="OO544_001032"
     CDS             13848..14552
                     /locus_tag="OO544_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCR6766688.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            14757..15470
                     /locus_tag="OO544_001033"
                     /pseudo
     CDS             14757..15470
                     /locus_tag="OO544_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
     gene            complement(15535..16246)
                     /locus_tag="OO544_001034"
                     /pseudo
     CDS             complement(15535..16246)
                     /locus_tag="OO544_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308893.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
     gene            complement(16246..17487)
                     /gene="clsB"
                     /locus_tag="OO544_001035"
     CDS             complement(16246..17487)
                     /gene="clsB"
                     /locus_tag="OO544_001035"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HCR6766689.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DNVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGDAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRNFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            complement(17484..18245)
                     /locus_tag="OO544_001036"
     CDS             complement(17484..18245)
                     /locus_tag="OO544_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCR6766690.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            18378..18788
                     /locus_tag="OO544_001037"
     CDS             18378..18788
                     /locus_tag="OO544_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HCR6766691.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(18750..19856)
                     /locus_tag="OO544_001038"
     CDS             complement(18750..19856)
                     /locus_tag="OO544_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6766692.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(19867..21000)
                     /locus_tag="OO544_001039"
     CDS             complement(19867..21000)
                     /locus_tag="OO544_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6766693.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(20993..22729)
                     /locus_tag="OO544_001040"
     CDS             complement(20993..22729)
                     /locus_tag="OO544_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6766694.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGNATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKETTPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKHGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(22722..23717)
                     /gene="hlyD"
                     /locus_tag="OO544_001041"
     CDS             complement(22722..23717)
                     /gene="hlyD"
                     /locus_tag="OO544_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HCR6766695.1"
                     /translation="MKKPVVIELAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERYLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGNEAGHE"
     gene            complement(23720..24391)
                     /gene="cecR"
                     /locus_tag="OO544_001042"
     CDS             complement(23720..24391)
                     /gene="cecR"
                     /locus_tag="OO544_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HCR6766696.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELLLRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGSDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            24620..25984
                     /gene="rhlE"
                     /locus_tag="OO544_001043"
     CDS             24620..25984
                     /gene="rhlE"
                     /locus_tag="OO544_001043"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HCR6766697.1"
                     /translation="MSFDYLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGGRGQGGGRGQQQPRRGEGGAKSASAKPAEKPSRRLGDAKPAGEQQRRRRPRKP
                     AAAQ"
     gene            complement(26216..26698)
                     /gene="ybiA"
                     /locus_tag="OO544_001044"
     CDS             complement(26216..26698)
                     /gene="ybiA"
                     /locus_tag="OO544_001044"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HCR6766698.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAAWPIKVDGKTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRNRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRVLLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            26818..28968
                     /gene="dinG"
                     /locus_tag="OO544_001045"
     CDS             26818..28968
                     /gene="dinG"
                     /locus_tag="OO544_001045"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HCR6766699.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRVPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTANNQEEQKRCAKLK
                     GDLDTYRWDGLRDHTDIVIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRVE
                     PSIDNEEQHIAEMAAFFRKQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            28996..29958
                     /gene="ybiB"
                     /locus_tag="OO544_001046"
     CDS             28996..29958
                     /gene="ybiB"
                     /locus_tag="OO544_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HCR6766700.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGAALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDTAGSELLPQAKDPETTAQ
                     WIERCLAGSEPIPESLKIQMVCCLVATGEAATISDGLARVNQAF"
     gene            30099..31184
                     /gene="hcxB"
                     /locus_tag="OO544_001047"
     CDS             30099..31184
                     /gene="hcxB"
                     /locus_tag="OO544_001047"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HCR6766701.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKTVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQKQGIPLDAGS
                     WQAICDAARQIGMPEETLQAFCQQLAS"
     gene            complement(31413..31673)
                     /locus_tag="OO544_001048"
     CDS             complement(31413..31673)
                     /locus_tag="OO544_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR6766702.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPITASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(31938..32204)
                     /gene="ybiI"
                     /locus_tag="OO544_001049"
     CDS             complement(31938..32204)
                     /gene="ybiI"
                     /locus_tag="OO544_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HCR6766703.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLDECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDSQLR"
     gene            complement(32278..32955)
                     /gene="ybiX"
                     /locus_tag="OO544_001050"
     CDS             complement(32278..32955)
                     /gene="ybiX"
                     /locus_tag="OO544_001050"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HCR6766704.1"
                     /translation="MMYHIPGVLSPQDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSQHCV
                     PPVTRGVRVASFMWIQSMIRDDKKRAMLFELDNNIQSLKSRYGESEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(32997..34981)
                     /locus_tag="OO544_001051"
                     /pseudo
     CDS             complement(32997..34981)
                     /locus_tag="OO544_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
BASE COUNT         8274 a         8975 c         9488 g         8244 t
ORIGIN      
        1 gaagaggtta atctttgcag ggcttgcaga gtgcgagtcg acaatggatt gttataaata
       61 ttaacatctg cataaatgtg cagattcatg atactgtctg gcaatgtgtt cagctgattg
      121 ttagggatat atagtgattg cagacgttgt ggaagttcgg gcagtctgtg tagttggttt
      181 tccccaacgt ttaaaagcct cagattttga ggaaggggcg gtagctcttg taatctgtta
      241 tcaaaagcac ttagttcctg tagttcacat ggtaattctg gtaaggcagt aagctggttg
      301 ttgccaacag aaagtttttg tagagccggg ggaagatcag gtagcgtctc caggctattg
      361 agaagggctg agagtgactg caaggaagat ggaagagaag gcaaacatgt taaaactctg
      421 ttaagtgaaa catcaagagc gaccaggtga ggcggaagag ccgggagcct gctcagtctg
      481 ttatcgctgg ctttaagtac agttaaggac ggagggagtt ctggcagaga gcgtagctca
      541 ttgctagaga tgttaagttc ttgtatgtgc gggggcaggt atgggagaga gcgtaatctg
      601 agtaaactta aattgagggc tggctcttga aaagccagac atattttcag taatcgaact
      661 gcctgtgttc ggtcttctgt tgcagcacct tccttggccc agttatccca gatgcggtga
      721 taatccgtga tattttgctc ttctacggat aaacgggata tacatcctaa accacaaaag
      781 gagcggaaaa aagaaggatt gtgggcaggt ttcatttatt attgatcctc attgtttata
      841 tattaatatg ttcgtgcaaa tggagtgagg attctttaaa aaaacgcgcc tgtaaatagt
      901 taagaacagg aagaactgtt ggggatgtgg ctggccgaaa atgaaggggt gaagttctgg
      961 ctcaatgtgc tgactgaact gaaaaaacgc ggttggtcgc cctacctttt catcaagcca
     1021 gtagtctgcc caccactgca tcatttctct gcgcttatcg agatactgag catggttgta
     1081 aattcagcta acccagtgcg ttgacaaaaa attagcgcaa gaggacaaaa aatcaccttg
     1141 cgctaatgct ctgtatcagg tcactaatac catctaagtg gttgattcat agtgactgga
     1201 tatgttgtgt tttgcagtat tatgcagtct attttttaag ctaaatgtat tataatgcat
     1261 tgatattaat gatttttact gtttctcgtt cagcttttta tactaacttg agcgaaacgg
     1321 gaaggtaaaa agacaaaaag ttgtttttaa tacctttaag tgataccaga tggcattgcg
     1381 ccatctggca gagtgattaa ctaaacatcg cagtaatcga ggcgcttgcc agagagtgga
     1441 aatgaacgtt aaacccgacc atcgcgccgc tggcaccttc atcgacatca atacgttcta
     1501 tatccagcgc gtgaacggta aaaatgtagc gatgagtttc gcctttcggc ggtgctgcgc
     1561 catcgtaccc ggttttacca aagtcggtac gcgtctgcaa aacgccgtct ggcattgcta
     1621 ccagaccaga gccaaaccct tgcggtaata cgcgggtatc agcgggtaag ttaacaacta
     1681 cccagtgcca ccagccggag ccggttggcg catccgggtc gtagcaggtg acaacaaaac
     1741 ttttcgttcc cgcaggaaca tcatcccacg ccagatgcgg tgaaatatta tcgccatcgt
     1801 aacccatgcc gttaaagaca tgacgatgcg gcaatttatc gccatcgcgc agatcgttac
     1861 tgatgagttt catgaaccct cctttcttgt ttgcagaaag tgtagccaga aaccctcacg
     1921 cggacttctc gttattggca aaaaaatgtt tcatcctgta ccgcgcggtt aaccgctgcg
     1981 gtcagacgct gcaactgttg cgggagaata atatagggcg gcatcaggta aatcagtttg
     2041 ccaaaaggcc ggatccagac accctgttcg acaaagaatt tttgcagcgc cgccatattc
     2101 accggatgag tggtttcgac cacgccaatg gcccccagta cgcgcacatc ggcaaccatt
     2161 tcggcatcac gggcgggggc aagttgctcg cgcagctgta cttcaatatc cgccacctgt
     2221 tgctgccagt cgccagattc gagaatcgcc aggctggcgt ttgctgccgc gcaggccagc
     2281 ggattgccca taaaagttgg cccatgcata aagcaaccgg cttcaccgtt actgatggtt
     2341 tctgcaacct cgcgcgtggt gagtgtggcg gaaagggtca ttgtgccgcc ggttaaggct
     2401 ttaccgaggc acaaaatgtc cggcgcgatt tctgcatgtt cacaggcaaa cagtttcccg
     2461 gtacgaccaa atccagtggc gatctcgtcg gcaatcagca agataccttc gcgatcgcat
     2521 attttgcgga ttcgttttaa ccattccgga tggtacatgc gcatcccgcc tgcgccctgg
     2581 acaatcggct caatgatcac cgccgcgatt tcatgacgat gcgccgccat caggcgggca
     2641 aagcccacca tatcgcgctc atcccattcg ccatccatac ggctttgtgg ggcgggagca
     2701 aacaagtttt ctggcagata gcctttccac aggctgtgca ttgagttgtc cggatcgcac
     2761 accgacatcg cgccaaaggt atcgccatga taaccattgc ggaaggtcag aaaacgctgg
     2821 cgcgcttcgc ctttggcttg ccagtactgc aacgccattt tcatcgccac ttccaccgct
     2881 acggaaccgg agtccgcgag aaaaacgcac tccagcggtt gcggcgtcat cgccaccagt
     2941 ttgcggcaca gctcaatggc tggcgcatgg gtgataccgc caaacatcac atgcgacatg
     3001 gcatcaattt gcgacttcat cgccgcatta agctgcgggt gattgtagcc gtggatcgcc
     3061 gcccaccagg acgacatacc gtcaaccagg cgtctgccgt cagacaaaat cagctcgcaa
     3121 ccttcggcgc tcaccaccgg ataaaccggc agcggggagg tcatggatgt gtatgggtgc
     3181 cagatatggc gttggtcaaa ggcaagatcg tccgttgtca taatcgactt gtaaaccaaa
     3241 ttgaaaagat ttaggtttac aagtctacac cgaattaaca acaaaaaaca cgttttggag
     3301 aagccccatg gctcaccgcc cacgctggac cttgtcgcaa gtcacagaat tatttgaaaa
     3361 accgttgctg gatctgctgt ttgaagcgca gcaggtccat cgccagcatt tcgatcctcg
     3421 tcaggtccag gtcagcacgt tgctgtcgat taagaccgga gcttgtccgg aagattgcaa
     3481 atactgcccg caaagctcac gctacaaaac cgggctggaa gccgagcggt tgatggaagt
     3541 tgaacaggtg ctggagtcgg cgcgcaaagc gaaagcggca ggatcgacgc gcttctgtat
     3601 gggcgcggcg tggaagaatc cccacgaacg cgatatgccg tacctggaac aaatggtgca
     3661 gggggtaaaa gcgatggggc tggaggcgtg tatgacgctg ggcacgttga gtgaatctca
     3721 ggcgcagcgc ctcgcgaacg ccgggctgga ttattacaac cacaacctgg acacctcgcc
     3781 ggagttttac ggcaatatca tcaccacccg cacttatcag gaacgcctcg atacgctgga
     3841 aaaagtgcgc gatgccggga tcaaagtctg ttctggcggc attgttggct taggcgaaac
     3901 ggtaaaagat cgcgccggat tattgctgca actggcaaac ctgccgacgc cgccggaaag
     3961 cgtaccaatc aacatgctgg tgaaggtgaa aggcacgcca ctggcggata acgatgatgt
     4021 cgatgccttt gattttattc gcactattgc ggtagcgcgg atcatgatgc cgacctctta
     4081 cgtgcgcctt tctgccggac gcgagcagat gaatgaacag actcaggcga tgtgctttat
     4141 ggctggcgca aactcgattt tctacggttg caaactgctg accacgccga atccggaaga
     4201 agataaagac ctgcaactgt tccgcaaact ggggctaaat ccgcagcaaa ctgccgtgct
     4261 ggcaggggat aacgaacaac agcaacgtct tgaacaggcg ctgatgaccc cggacaccga
     4321 cgaatattac aacgcggcag cattatgagc tggcaggaga aaatcaacgc ggcgctcgat
     4381 gcacggcgta ctgccgatgc cctgcgtcgc cgttatccgg tggcgcaagg agccggacgc
     4441 tggctggtgg cggatgatcg ccagtatctg aacttttcca gtaacgatta tttaggttta
     4501 agccatcatc cgcaaattat ccgcgcctgg cagcagggcg cagaaaagtt tggtgtaggt
     4561 agcggcggtt ccgggcatgt cagcggttat agcgtggtgc atcaggcgct ggaagaagaa
     4621 ctggccgagt ggcttggcta ttcgcgggcg ctgctgttta tctctggttt tgccgcgaat
     4681 caggcagtca ttgccgcgat gatggcgaaa gaggaccgta ttgttgccga ccggcttagc
     4741 catgcctcct tgctggaggc tgccagttta agcccgtcgc agcttcgccg ttttactcat
     4801 aacgatgtcg ctcatctggc gcgactgctt gcttccccct gtccggggca gcaactggtg
     4861 gtgacagaag gcgtgttcag catggacggc gatagcgcgc cactggcgga aatccagcag
     4921 gtaacgcaac agcacaatgg ctggttgatg gtcgatgatg cccacggcac gggcgttatc
     4981 ggggagcagg ggcgcggcag ctgctggctg caaaaggtaa aaccagaatt gctggtggtg
     5041 acttttggca aaggatttgg cgtcagtggg gctgcggtgc tttgctccag tacggtggcg
     5101 gattatctgc tgcaattcgc ccgccacctt atctacagca ccagtatgcc gcccgctcag
     5161 gcgcaggcat tacgtgcgtc gctggcggtc attcgccgtg atgaggggga tgcacggcgc
     5221 gaaaaactgg tgtcactcat tgcgcgtttt cgtgccggag tacaggatct gccgtttacg
     5281 cttgctgatt catgcagcgc catccagcca ttgatcgtcg gtgataacag ccgtgcgtta
     5341 caactggcag aaaaactgcg ccagcaaggc tgctgggtca cggcgattcg cccgccaacc
     5401 gtacccgctg gtactgcgcg actgcgctta acgctaaccg cagcgcatga aatgcaggat
     5461 atcgaccgtc tgctggaggt gctgcatggc aacggttaat aaacaagcca ttgcagcggc
     5521 atttggtcgg gcagccgcgc actatgagca acacgcagag ttacagcgcc ggagtgctga
     5581 cgtcttactg gcaatgctcc cacagcgtaa atacacccac gtactggacg cggggtgtgg
     5641 gccaggctgg atgagccgcc actggcgaga gcgtcacgca caggtgacgg cgttagatct
     5701 ctcaccgcca atgcttgtgc aagcccgcca gaaagatgct gcagaccatt atctggcggg
     5761 agatatcgaa tccctgccgt tagcgactgc gacgttcgat cttgcatgga gcaatctcgc
     5821 ggtgcagtgg tgcggtaatt tatccacggc actccgcgag ctgtatcggg tggtgcgccc
     5881 cggaggcgtg gtggcgttta ccacgctggt gcagggatcg ttacccgaac tgcatcaggc
     5941 gtggcaggcg gtggacgagc gtccgcatgc taatcgcttt ttaccgccag atgaaatcga
     6001 acagtcgctg aacggcgtgc attatcaaca tcatattcag cccatcacac tatggtttga
     6061 tgatgcgctc agtgccatgc gttcgctgaa aggcatcggt gctacgcatc ttcatgaagg
     6121 gcgcgacccg cgaatattaa cgcgttcgca attgcagcga ttgcaactgg cctggccgca
     6181 acaacagggg cgatatcctc tgacgtatca tctttttttg ggagtgattg ctcgtgagta
     6241 aacgttattt tgtcaccgga acggataccg aagtggggaa aaccgtcgcc agttgtgcac
     6301 ttttacaagc cgcgaaggca gtaggttatc ggacggcagg ttataaaccg gtcgcctctg
     6361 gcagcgaaaa gaccccggaa ggcttacgta atagtgacgc gctggcgtta cagcacaaca
     6421 gcagcctgca gctggattac gcaacagtaa atccttacac cttcgcagaa cccacttcgc
     6481 cgcacatcat cagcgcgcaa gagggcaggc cgatagaatc attggtaatg agcgccggat
     6541 tacgcgcgct tgaacaacag gctgactggg tgttagtgga aggtgctggc ggctggttta
     6601 cgccgctttc tgacactttc acttttgcag attgggtaac acaggaacaa ctgccggtga
     6661 tactggtagt tggtgtgaaa ctcggctgta ttaatcacgc gatgttgact gcgcaggcaa
     6721 tacaacacgc cggactgact ctggcgggtt gggtggcgaa cgatgttacg cctccgggaa
     6781 aacgtcacgc tgaatatatg accacgctca cccgcatgat tcccgcgccg ctgctgggag
     6841 agatcccctg gcttgcagaa aatccagaaa atgcggcaac cggaaagtac ataaaccttg
     6901 ccttgttgta gccattctgt atttggttaa attgcgagcg agatcgcgtc ttcgattgac
     6961 tgcaatttaa ccaattaaat tctaaaataa tcacgaaaaa aattttactt ctgcctcatg
     7021 cggcgaatgt gggaattgcc caggcggcgg gggatagggg ctggagacag ttatccacta
     7081 ttcctgtgga taaccatgtg tattagagtt agaaaacacg aggcaagcga gagaatacgc
     7141 ggcttgcacg cgaattggcg ttaaagacgg ctcaaagaaa tatcttttat tttttaactg
     7201 gttagataaa tgcaatggca gtcactgaac aggcatctct tgccataaaa ctgtcatcac
     7261 tcatcttgac aaatgttaaa aaagccgttg ctttggggat aacccggtaa ggccggagtt
     7321 ttatctcgcc acagagtaaa ttttgctcat gattgacagc ggagtttacg ctgtatcaga
     7381 aatattatgg tgatgaactg tttttttatc cagtataatt tgttggcata attaagtacg
     7441 acgagtaaaa ttacatacct gcccgcccaa ctccttcagg tagcgactca tgagtaaacc
     7501 gttcaaactg aattccgctt ttaaaccttc tggcgatcag ccagaggcga ttcgacgtct
     7561 cgaagagggg ctggaagatg gcctggcgca ccagacgtta cttggcgtga ctggctcagg
     7621 gaaaaccttc accattgcca atgtcattgc tgaccttcag cgcccaacca tggtacttgc
     7681 gcccaacaaa acgctggcgg cccagctgta tggcgaaatg aaagagttct tcccggaaaa
     7741 cgcggtggag tatttcgtct cctactacga ctactatcag ccggaagcct atgtaccgag
     7801 ttccgacact ttcattgaga aagatgcctc ggttaacgaa catattgagc agatgcgttt
     7861 atccgccacc aaagcgatgc tggagcggcg tgatgtggtt gtagtggcct ctgtttccgc
     7921 gatttatggt ctgggcgatc ctgatttata tctcaagatg atgctccatc tcacggtcgg
     7981 tatgattatc gatcagcgcg cgattctgcg ccgactggcg gagctgcaat acgctcgtaa
     8041 tgatcaagca ttccagcgtg gtactttccg cgttcgtggc gaggtgatag atatcttccc
     8101 ggcagaatcg gatgacattg cacttcgcgt ggaactgttt gacgaggaag tggaacgatt
     8161 gtcgttattt gacccgctga ccgggcagat tgtttccact attccacgtt ttaccatcta
     8221 cccgaaaacg cactacgtca caccgcgcga gcgcatcgta caggcgatgg aggagatcaa
     8281 agaagaactg gccgccagac gcaaagtgct gttggaaaac aacaaactgc tggaagagca
     8341 gcggctgacc cagcgtaccc agtttgatct ggagatgatg aacgagctgg gctactgttc
     8401 ggggattgaa aactactcgc gcttcctctc cggtcgtgga ccgggtgagc caccgccgac
     8461 gctgtttgat tacctgcctg ccgatgggct gctggtggtc gatgaatctc acgtcaccat
     8521 tccacaaatt ggcggcatgt atcgcggtga ccgggcgcgt aaagagacac tggtggagta
     8581 cggcttccgc ctgccatcag cgctggataa ccgtccgctt aagtttgaag agttcgaagc
     8641 attagcgccg caaaccatct atgtttcggc gacgccgggt aattacgagc tggaaaaatc
     8701 cggcggcgat gtggtggatc aggtggtgcg tccaaccgga ttgcttgacc cgattatcga
     8761 agtgcggccg gtggcgacac aggttgatga tcttctttcg gagattcgtc agcgagcggc
     8821 aattaacgaa cgcgtactgg tcaccacact gaccaagcgg atggcggaag atcttaccga
     8881 atatctcgaa gaacatggcg agcgcgtgcg ttatcttcat tcagatatcg acaccgtcga
     8941 acgtatggag attatccgcg acttgcgtct gggtgagttc gacgtgctgg tagggatcaa
     9001 cttactgcgc gaaggtctgg atatgccgga agtgtcgctg gtggcgatcc tcgacgctga
     9061 caaagaaggc ttcctgcgtt ccgaacgttc gttgatccag actattggtc gtgcggcacg
     9121 taacgttaac ggtaaagcga ttctctacgg cgataagatc accccatcaa tggcgaaagc
     9181 gattggcgaa accgaacgtc gccgtgagaa acagcagaag tacaacgagg aacacggaat
     9241 tacgccgcaa ggcttgaaca agaaagtggt cgatatcctg gcgctggggc agaacattgc
     9301 caaaaccaaa gcgaagggca gaggaaaatc gcgcccgatt gttgagccgg ataatgtgcc
     9361 gatggatatg tcgcctaaag cgttgcaaca gaaaatccat gagctggaag ggttaatgat
     9421 gcaacacgcg cagaatctgg agttcgaaga agcggcacaa attcgtgacc agttgcatca
     9481 gctgcgtgag ctgtttatcg cggcttcgtg acaggataac gaagaagaca gatgacaaaa
     9541 gcaaaattgc ctgatgtgct acgcttatca ggcctacatg atctctgcaa tacattgaat
     9601 tagcgcggtt tgtaggccgg ataaggcgtt cacgccgcat ccggcataaa caacgcgcac
     9661 tttgtcttca ataggaagcc ggaatttttc ttcggatttc cgttaaccta aagcctgtaa
     9721 cgccttttcc agcgcgttat gtaacaactg gcggtcatga cgatacggaa tatcgctggc
     9781 ctccagtacc tcctgaatca caatccgctc tttcaccgcc gagacatcca cttttggccc
     9841 gacgatgacc gcatcaatga cctttttacc aacatactgc tccataattg ccagcttgct
     9901 ttccagcttc aaattagccg caggtaaact caactcacgc cccagattgc cgatatagac
     9961 catcggcgct ggcgtgcggc gtaatgcctg ggcgatttcc ttcagcagca gaattggcat
    10021 caggctggta taaaaactgc caggcccaat aatgatgaga tccgcttcat tgatagcgtg
    10081 aaccgcctca cgcgttgcgg gtacattcgg cgttaataat aactcttgaa tcggcgtagt
    10141 taactggtcg atattgacct cgccgtaaac ttcatgcccc tgatcgtcaa tcgccatcag
    10201 atcgacggga tgctctgaca ttggaatcaa atgcgtatcc actttcagca gattacgaat
    10261 taaattgatg gcttccagag gccgcacgct aaggtgatcc agcgccttta acatcaagtt
    10321 tccgagatta tgaccggaaa gttcgccatt gccgccaaaa cggtattcaa acatcgcgga
    10381 ggcgacgctc ggttccgtta tcagttggtt gaggcagttg cgcatatcgc cccaggcaat
    10441 gccgccttct gaacggcgaa tacgccccgt cgagccacca ttatccgtgg tggtgacgat
    10501 acccgttaaa cgagaaccca aagacgaaag tgatgagaga acgcgtccca gtccatgccc
    10561 tccgccgaga gcaacgacac gatcaagatc agccagcgta cgattgcgca tatagtttcc
    10621 tggagtcaga ttatccgcgc tacagtagcg caaattgcgg ggaaacagca attaacctgc
    10681 caatattgag gatacaaact attttcttct acctctaaag gacgatgcac gctatgcctc
    10741 cctgatgatg tatatcaaag taaaactgcc attttccctt attctgtagc gaaatagcac
    10801 gatcatgacg ctatatacat gattacatag cgaaagtgtg gatggtaaaa atctcattta
    10861 cacgctagta tcggcttaac cactaaacac tctagcctct gcacctgggt caactgatac
    10921 ggtgctttgg ccgtgacaat gctcgtaaag attgccacca gggcgaagga agaaatgact
    10981 tcgcctcccg tatctggaaa ggtgtacatg gcttcacaac tgactgatgc atttgcgcgt
    11041 aagttttact acttgcgcct gtcgattacc gatgtgtgta actttcgttg cacctactgc
    11101 ctgccggatg gctacaaacc gagcggcgtc accaataaag gctttcttac cgtcgatgaa
    11161 attcgccggg ttacgcgcgc cttcgccagt ctgggcaccg aaaaagtgcg cctgacagga
    11221 ggagagccgt ctttacgccg cgactttacc gatatcatcg ccgctgtgcg ggaaaacgac
    11281 gctatccgcc agattgcggt caccaccaat ggttaccgtc tggaacgcga tgtggcgaac
    11341 tggcgcgatg cgggacttac tggcattaac gtcagtgtcg acagtctgga cgcccgccag
    11401 tttcacgcta ttaccgggca ggataaattc aaccaggtca tggcagggat cgatgctgca
    11461 tttgaggtcg gttttgagaa ggtcaaagtc aataccgtgc tgatgcgtga tgttaatcat
    11521 caccagctcg acacctttct gaactggatc cagcatcgcc ctatccagct gcgtttcatc
    11581 gaactgatgg aaacgggcga gggcagtgag ctcttccgta agcatcacat ctctggtcag
    11641 gttctgcgtg acgagctact gcgtcgcggc tggatccacc aattacgtca acgcagcgac
    11701 ggtcctgcgc aagtcttttg ccatccagat tacgccggag agattggcct tatcatgccg
    11761 tatgaaaaag acttctgcgc cacttgcaac cgcctgcgcg tttcctccat tggtaaactc
    11821 catctctgcc tgtttggtga aggcggcgtt aacctgcgcg atctgctgga agacgatacc
    11881 cagcaacagg cgctggaagc gcgtatttca gcggcgctgc gggagaagaa acagacccat
    11941 ttcctgcatc aaaacaacac cggtattacg caaaacttat cgtacattgg cggctaaaac
    12001 gtcaaaagga gagatcagat gagtcaggta agcactgaat ttatcccgac ccgtattgct
    12061 attcttacgg tttctaatcg tcgcggtgaa gaagacgata cctccggtca ctatttgcgc
    12121 gattcggcgc aagaagcggg ccatcacgtt gtcgataaag ccattgtgaa agaaaaccgc
    12181 tacgctattc gcgctcaggt atctgcgtgg atcgccagcg acgatgtaca agtggtgttg
    12241 attacggggg ggactggcct gacggaaggt gatcaggctc ccgaagcatt gctgccgttg
    12301 ttcgaccgtg aagttgaagg ttttggtgaa gtgttccgga tgttgtcgtt tgaagagatt
    12361 ggcacttcca cgttgcaatc tcgtgcggta gcgggcgtcg ccaacaaaac gctgattttc
    12421 gccatgccgg gttcgaccaa agcgtgccgt accgcatggg aaaatatcat cgcgccgcag
    12481 ctggatgccc gtacgcgtcc gtgtaatttc catccacatt tgaagaaata agtatgtcgc
    12541 aactgaccca tatcaacgcc gctggcgaag cgcacatggt ggatgtctcc gccaaagcgg
    12601 aaaccgtgcg tgaagcgcgg gcggaagcct ttgtcaccat gcgcagcgag acgctggcga
    12661 tgattattga tggtcgccac cacaaaggcg acgtatttgc cactgcgcgt attgtcggta
    12721 ttcaggcggc aaaacgcacc tgggatctga tcccgctgtg tcacccgctg atgctcagca
    12781 aagttgaagt caatttacag gccgagccgg agcacaatcg ggtgcgtata gaaaccttat
    12841 gccgcctgac cgggaaaacc ggtgtcgaaa tggaagcgtt aaccgcggcc tccgtggcgg
    12901 cgctgaccat ttatgacatg tgcaaagcgg tgcaaaaaga tatggtgatt ggtccggtac
    12961 gtttgctggc gaagagcggc ggcaagtcgg gtgactttaa ggtggaagcg gatgattaaa
    13021 gttctttttt tcgcccaggt gcgcgagttg gtgggaacag atgcaaccga agtggctgcg
    13081 gattacccaa ccgttgaagc gttacgccag cacctggctg cgcagagcga tcgctgggcg
    13141 ctggcgctgg aagatggcaa attgctggct gccgtcaacc agacgctggt gagttttgac
    13201 catccgctga ctgacggcga cgaagtagct ttcttcccgc cggtaaccgg aggttaagat
    13261 ggcagaaacc aaaattgttg tcggcccgca gccgttcagc gtaggagaag agtacccgtg
    13321 gctggctgag cgtgacgaag acggtgcagt ggtcacgttt accggtaagg tgcgcaacca
    13381 taacctgggt gacagcgtca aagcattaac cctcgaacac tatccgggga tgactgaaaa
    13441 agcactggca gaaatcgttg atgaagcgcg taaccgctgg ccgctggggc gcgtcacggt
    13501 gattcaccgc atcggggaat tatggccggg cgatgaaatc gtttttgtcg gtgtcaccag
    13561 tgcgcatcgc agcagtgcgt ttgaagccgg gcagtttatt atggattatc tcaaaacccg
    13621 cgcaccgttc tggaagcgcg aagccacgcc ggaaggcgac cgctgggttg aagcccggga
    13681 gagcgatcag caggcggcaa aacgctggta gttttttgtt agccggataa ggcaccgctg
    13741 cgtccggcaa tttcaccgct cagtacatgc gtttacgctg cgtcgatgca cagcctcatc
    13801 attttgcagt atccttaaga tattccttat atcttcagga gatcgtcatg gacagattcc
    13861 cacgttctga ttcaatcgta caaccccggg ctggcttgca aacttatatg gcgcaagtct
    13921 atggctggat gaccgttggc ttgttgctga ccgcatttgt tgcctggtat gcggctaatt
    13981 ccgcggccgt gatggagctg ttgttcacta accgtgtctt tttaatcggt ctgatcatcg
    14041 cgcaattagc attggttatt gtgttatcag cgatgattca aaagctgagc gcaggtgtaa
    14101 cgacgatgct ctttatgctt tattcggcgc tgacgggtct tacgctttcc agtatattca
    14161 ttgtctatac cgctgcttct atcgccagta ctttcgtcgt tactgccggg atgttcggcg
    14221 caatgagcct gtacggttac accacgaagc gcgatttaag tggctttggc aatatgctgt
    14281 ttatggcgtt aatcggcatt gtgctggcat cgctggtcaa cttctggctg aaaagcgaag
    14341 cattgatgtg ggcagttacc tacatcggcg tgattgtctt tgtcggattg acggcgtatg
    14401 acactcagaa attgaaaaat atgggtgagc agattgatac ccgcgacacg tcgaacctgc
    14461 gcaaatattc cattcttggc gcgttaacct tgtatctgga cttcatcaac ctgttcctga
    14521 tgttgttacg gatcttcggc aaccgccgtt aattcttttt caccatcact atcccggcca
    14581 tgtgtcggga tttttttctt actaaaaacg acacttaaca agtaccaggt ataaaatatg
    14641 ccttatgagc gagcggaatc gttttccgta tccataaata caaaagtatt ttgtatgcgt
    14701 ggttttaaat ttattgatgg tgaatattaa tattttttct aaggacaact aaaagaatgg
    14761 aatcatactc gcaaaacagc aataaattag attttcagca cgaggccagg atataaaacg
    14821 gtatatggct cattaccgct ttaggtttgg tggcaaccgc aggactagcc tggggagcta
    14881 agtatctcga aattacggca accaaatatg attcaccacc aatgtatgtc gctatagggt
    14941 tattattact ttgtatgtat ggcttaagta aggatatcaa caagataaat gccgccatcg
    15001 cgggcgtaat atatctgttt ttactctctt tggtggcgat tgtcgttgca agtttagttc
    15061 ctgtatatgc cattatcatc gtgttcagca ctgcgggcgc gatgttttta atcagtatgc
    15121 tggccggttt attatttaat gttgagcctg gttctcaccg ttttatcatt atgatgacgt
    15181 tgacagggtt ggccctggta atcatcgtga atgcggcatt aatgagtgaa cggcccattt
    15241 gggtaataag ttgcttaatg attgtgttat ggccaggcat tatctcgcat ggacgaaata
    15301 agctccttga actggcagga aaatgccata gtgaagagtt gtggagtccg gttcgttgcg
    15361 cttttacagg tgcattaaca ctctattact attttatcgg cttctttggg atacttgccg
    15421 cgatagctat aacgcttgtc tggcaaaggc atacgcgttt ttttcattag ccgtaaatgc
    15481 acatactaaa gaacttaact aaacttcaca tcgccgcttc atttttcgcc cgtagcccag
    15541 tacaaagaaa tagttcacgc tttgaccaag taacagccag ataatcgccc ccattaccat
    15601 ccagttaacg ctggaaatca gcatctgagc gagggcgaat ttccatgaag gcagcaccag
    15661 cttttgccct ttgatggtca tatggcggtg cttcgcgaaa gcgcaaaacc acaaataaac
    15721 cgcgataatc atcagtaagc caatgccgag aatacgcagc gtagtttgat cgacatacca
    15781 gtgatccggc aactccacca cgcctgcggt aaagataatc cctgccagta aaatatagcc
    15841 cagccagttg gtggtaatac tgagcgagaa aatccgcgta atagtgctac cgggcaaccc
    15901 cagacgagag tacaaacgat agcgcatacc aatgccgccg acccaggtac tgagcgtcag
    15961 gttgaaggcg tagcagataa acgacaccag catcacctgg cgcttcgcca gtttgtgacc
    16021 gcagtaaaag cgggcgagca ggtcatagca gccgtaaatc agatagctga cgaccaccag
    16081 cgaccgcact aagcagcgca acgcgattgt agtcgcggat gaccttccag acctcttccc
    16141 agtccacttt tttggcgtag accaccagca acacaatcac cgcgataaaa aacagccagg
    16201 tgaggatctt ctttgctaag cgccagcgcg ggtgtgattt actcatcagg gttttacccc
    16261 cgtgttttca gtttctaccc gatcctgcgt ttccattgtc ggttgtgcgg gcggatcaac
    16321 ctgcgccaga cgtggcgtgt gtgccggaag ccagccaacc agcgccggga agtggcgtaa
    16381 aaagtggaac gccagcacgc ttttggtcag gttccaccag gtgcgtttgg gcagcatggt
    16441 ttcatccacc tgctgacaat ctgcggcgat aatgccgttc agattatcgc gcagcgtctg
    16501 gttaaaatta cgatcgtgga tgatgacatt tgcttcgaga ttcagtgaca aactgagcgg
    16561 atcgagatta ctggacccta ctgtcgccca gtgatcgtcc atcaatgcta ctttgccgtg
    16621 gagcgggcgg cggcggtact caaacacctg aacgccgcct ttaaccagat agttatacag
    16681 caagcgcgcg ccgactctga caatcggcat atccggttcg ccctgaatga tcagtttgat
    16741 ccgcaccccg cggcgtgccg ctttacgcaa ggcgtgtaaa aatcgatagc cggggaagaa
    16801 gtaggcgttg gcgataatga cttcccgccg cgcctgagtg agcattttca gatagtggcg
    16861 ttctatatca tcgcggtgtt cttcgttatc gcgccagacc agcaatacct gcgcgtctcc
    16921 cggctggcgg ttctcttccg ctttgtgatg acgtcgccac cagcgtcgtg ccgcgctctg
    16981 tccaggcagg ttttccagct caaactggag aatatcttcg acaatcggcc cttcaaggcg
    17041 taccgcgtaa tcctgtttag cctctggacc gtagctggac atatgctcgg cggagtaatt
    17101 cagcccgcca ataaaggcga tacgcgcgtc gatcaccaca attttgcgat gcatccggcg
    17161 aaacacattg gtgcgcatac caaaaaggcg agggcgggga tcgtagtagc ggaacactac
    17221 gccagctgcc gtcagttcat tgacaaactc atcgctgaga tccggcgaac cgtagccatc
    17281 cagcaagact tccgctttaa ccccgcgttg cgctgctgcc agtagtgccg catgcagctg
    17341 tttgccgacg ttatcctcaa accagataaa cgtttcaaga atgatgcgtt cttgtgcctc
    17401 gccaatcgcc ttaaacaccg cgggataata ttgctcgccg ttttccagca actggatctt
    17461 attgccttcg cgccagctac atttcataaa tgaatctccg cacttaaagg ggcatgatca
    17521 gaaaggtgtc gccatgtccg cagcggcaac gcggttggcg cgctggcgct ggcatttttg
    17581 acgtagatcc tgtccagtcg tagtagagga aattgcaccg gaaacgtgcg cgccgggcgt
    17641 ccgtgggcgc gggtaaaaat ctcatccagt ccggcctgca cttttaacgg atgattagct
    17701 ttttgccgcc agtcattgaa atcacccgcc accaataccg gttcgccgtc cggtagctca
    17761 ttcacccatt cggcgagcat cgcaagctgc gcctgacggt gcgcctcacg caggcccaga
    17821 tgtacgcaca tcacatgaat cgcttttccg gtcatcggcg gcacaatgcg gcagtagagc
    17881 acgccgcgct tttccgcacc atcgaccgaa acatcgcgat tctcataatg ttcaatggga
    17941 taacgcgaca gtacggcgtt gccgtgatgc ccttccgggt atacggcatt gcgaccgtag
    18001 gcaaaatcgc tccacatagt gtcggcgaga aactcgtagt gcgaggtatc gggccagttt
    18061 tccacatgca gcggatgaac ttcgtgcgcg cccatcactt cctgcaggca aacaatatcg
    18121 gcgctgacgg tacgcacggc atcgcgaagt tccggcaaaa tgaagcgtcg gttaaacgcg
    18181 gtaaagcctt tgtgaatgtt gatggtgagc accttgaacg aaaattgttg tgtttgatcg
    18241 ggcataattt tcctgtcttt gcctcttatc tcattgaaat agtgtagtcg gcgtcacaaa
    18301 aaggtgcggt cttacggaat tttccgtaaa gttcggtact ctgagtaagt agagataaat
    18361 tcttcaggag agaagccatg aagtggcaac aacgtgttcg tgtcgcaacg ggtctaagtt
    18421 gctggcagat tatgttgcat ttactggtag tggcgctgct ggtggtgggc tggatgagta
    18481 agactctggt tcacgtcggc gtgggattat gcgcactgta ttgtgtcacg gtagtgatga
    18541 tgctggtgtt tcagcgccac cccgagcaac gctggcgtga ggtggcagac gtgctggaag
    18601 agctgaccac gacctggtat tttggcgcag cgctgattgt gctgtggctg ttgtcccgcg
    18661 tactggaaaa caactttttg ctggcaattg cagggctggc gatccttgct ggcccggcgg
    18721 tagtgtcttt gctggcgaaa gataagaagt tacatcacct tacgtctaaa catcgcgtac
    18781 gccgctgacc ctgtcgtggc cgttatcacc agtagcggcc acaaactatt ccacacaata
    18841 tccagactcg catccttcaa ataaatctgc ttggtaatgt ccgtaaagtg gcgaataggg
    18901 ttaatccacg tcaggttttg cagccatact ggcatgtttt ccaccggaga aacgtaaccg
    18961 gaaaggagaa tggcgggcat cataaaaaca aacacgccga taaacgcctg ctgttgtgtc
    19021 gagcagagtg atgaaatcaa cagaccgaat cccaccagcg ataaaccgta aatcaccatc
    19081 gtaaagtaga acagcgccag cgatccggcg aaggggattt gatacgccca gataccaatc
    19141 gccagcacaa tggtggcctg gaaggtggcg acaattaacg ccggtacggc tttgccgatg
    19201 aagatctgcc aggtggtgag cggcgaaacc agtagctgat cgagcgtacc ttgttcacgt
    19261 tcgcgggcga cggaaagtga agtgacgatc attacgccga tagtggtgat catggcgatc
    19321 agcgacggca ccacaaacca tttgtagtca agattcgggt tataccagtt gcgtaccacc
    19381 agctcgctgt tgttaggttt cggttttcct tccagcagct cctgctgata atttttgacg
    19441 atctgttgca ggtagttggc ggcaatttgc gcactgttgg agttacgccc gtcgaggatc
    19501 aactgcaacg gcgcggtctg gaaggtatcc agtttgcgcg agaagtcagc cgggaaacgc
    19561 accagtagta acgccttttg tgtgtcgatg gttgggcgga tctcctgtgg gcttttcagc
    19621 agcagcacat gggtaaaggc gctggcgcgg gcaaaacgtt gggtcagctc caccgaatgc
    19681 tcgccgttat cttcatcgta gatggcgatg gtggcgttag tcacttccag cgtggcggcg
    19741 aacgggaaca ggatcacctg aataagcaca ggtaaaatca gaatcgcgcg ggtttgtggt
    19801 tcgcgcagca acgactgcaa ctctttgcgg attaacgtcc ataagcgatg aaacatgctc
    19861 ttctccctaa tccagccgac gtttggtttt cagccacgtc aggccgataa acatcaccgc
    19921 cgaagcgatc aaaaacagca cgtttaccac cagcaccact ggaatattcc cggcgaggaa
    19981 caggctttgc agggtgctga cgaaataacg agcgggaata atgtacgtca ccgcgcggat
    20041 caccgcgggc atactgtcga tctgaaaaat aaagccggaa agcataatcg acggcaggaa
    20101 cgcggcgttc agggcgacct gagcggcatt gaactggttg cgggtaatcg tggaaatcag
    20161 cagccccatc cccagggtac tgagtaaaaa caggctggag ataaaaaaca gaataagcag
    20221 cgaaccgcga tacggcacgc cgagaataaa cactgacacc agcatacaca gcagcatcgc
    20281 cagcatcccg agaaagtaat aagggatcag cttacacagc agcagttccg tgcgggtaat
    20341 ctccgtagag agcagagcct ccatggtgcc gcgttcccat tctcgcgcca ccaccagcga
    20401 ggtgagaatc gcgccgatga ccgtcatgat gatggtcact gcaccgggga taatgaagtg
    20461 ctggctaatc gccgccgggt taaaccagta gcgggtttgt acatcaataa gcggttcaaa
    20521 agtctgcccg ttgtcctccg ctcgctgcat ttgccagatc tgccagatcc cttcgacata
    20581 cccctgtaca aagttagcgg tattcggctc actgccgtcg gtgatcacct gaatcggtgc
    20641 ggtggcgttg gcgcgctcca tctgttccgc aaaatccacc ggaataacca ccagaccgcg
    20701 aattttcccc gcctgcattt tggcgatcag ttcctgacgg ttatcgctga tggtggcgtc
    20761 gatgtagggc gaaccggtca tggtgtgggt gaaatccagc gccgcttcgc tacgctgttc
    20821 cagcaagatc ccgacccgca gcttgctgga gtcgaggtta atgccataac caaaaataaa
    20881 cagcagtagc agtgggatca ctaccgcaat cagccagcta ctcggatcgc gaacgatctg
    20941 ccgcgtctct ttgacgcaca gcgcccgcac gcgacgccag gaaaggatcg ggttactcat
    21001 tgctatgctc cttatcccag tcgtggatca actgaataaa ggcttgctcc atggtgggat
    21061 cgggttgctc atcgttagcc gactgtgctt tcaaatcgtc cggcgtaccg ctggcgatta
    21121 atttcccacg gtacaccagg ccgatgcggt cgcaatattc cgcttcatcc ataaagtggg
    21181 tggtgaccat caccgtgacg cctttctcta ccatgctgtt gatatgtagc caaaattcac
    21241 ggcgggtgag gggatcaacg ccggacgtcg gttcgtcgag aaacagaatg tccggttcat
    21301 gcatcagcga acaggccagc gccagccgct gtttaaaacc taatggcagt tcatcggtgg
    21361 cgtgggaggc gatacttttc aggccgaacg cctcgctcat gcgggagatt ttttcgttct
    21421 gcgcccgacc gcgtaagcca tacacaccag agaaaaagcg taaattctgt tcgaccgtca
    21481 ggttaccgta gagcgaaaac ttttgcgcca tatagccgag atgctggcgc gctttaccag
    21541 aactctcttt cagatccatc cccagtacca gcgcctgacc agaggtcggc accagcaaac
    21601 cgcacatcat cttaaaggtg gtcgatttgc ccgcgccgtt tggccccagc aaaccaaaaa
    21661 tctccccgtg tttaacggca aagttgacgt gatcggtggc ggcaaaatca ccaaatttct
    21721 tggtcagttc tttcgcttcg atcaccgtct cgccgggtgt gccttctacc gtatgtaata
    21781 ttgcgcccag cggcgattcc gaggttccgg caccgcccag caaatcaata aacgcatctt
    21841 caaaacgcgg cgtagtttcg ttgatgttga tttccggcat cccgtcggca tggcgaatat
    21901 cgtctggtgt ggtctctttt ttgaggatca gacgtaccga tttcccctga atcatgccgt
    21961 cgctgacctg cggcagtttc aatgcccgtt gcaacagttt gcggttgccc tcgtgtggac
    22021 ttgtcatcag aaagctgcgt ccggccatgg tttgtgtcag ggcttttggt tccccctgat
    22081 acagcaactc gccttcattc atcagtaaca cgtcacggca ctgctcggct tcgtcgagat
    22141 acgaggtact ccagaggatt aacatccctt cgcctgccag ctcatgcacc atctgccaca
    22201 gttcgcgccg tgagataggg tcaacgccga cgccgggttc atcgagcagc aacactttcg
    22261 gttcgcccac cagggtacag gccaggccga gtttttgttt catcccaccg gagagcttgc
    22321 ccgccaggcg tccggtaaac ggcccaagag acgtaaactc cagcaggcga gcaaaagttt
    22381 gcttacgtgc ctcgccggtg acgctgcgca aatccgcgta cagattgagg ttctccatca
    22441 ccgtgagatc ttcatacaga ccaaatttct gcggcatata gccgagcacg gcgtgcagcg
    22501 cgccgtcgtt tttgattgga tcaaagccaa tcaccgtggc attgccgctg tcgggtttca
    22561 gtaatcccgc cagcatccgc attagcgtgg ttttacctgc accatccggc cccaccagcc
    22621 ccgtcacata accggcgtga atggtacaat cgagcggcgc gacggcgggc ttgtccatgc
    22681 caggaaagcg tttttccagg ccgttcagcg tgataacggc atcattcatg tcctgcctcg
    22741 ttaccgaatt gcaccgtcac cggcattccc tggcgtaacg catcatcggc gtcggtcacc
    22801 acaatacgca ggcgatagac gaggtcggta cgcagatccg gtgtttcgac ggttttcggg
    22861 gtaaattcag cagtcggcga aacgaaacca atctgcccgt gatacggctt gtccgggcga
    22921 ccatcggtat aaagcagcac tttgcgcccc ggctgggcct ggtcaagata ccgttcatca
    22981 acataagcgc gcacccacac cggacgcgtt agtgaaacgg taaacaccgt gccaccttca
    23041 ttgaggaccg tgcctggctc caccgcgcgc gttaacagcg tgccatcaga cggggctatc
    23101 aacgttgagt cctgtaaatt caactccgcc tgcgccagtt gcgcctgcgc ctgttcgagg
    23161 ctggctttcg cctgggcgat gtcctgttca cggttaccgg aacggtactg acgcaattta
    23221 tcctgtgctg atttcagcgt tgcctgcgcc tggtcgcgcg aggagcgggc attttccagg
    23281 tcatttgccg aaatagtgcg gcttttccac agtccttgct ggcggttata gaagttctgc
    23341 gcatagtcat aggcggcttg cgcctgtttc accgctgcgg cggcctgagc gatttcttca
    23401 tcgcgatacc cggcaagcat cagatcatac tgcgcctgtg ccaccgagac gcccgctttc
    23461 gcctgcatca gggcaatctc atacggcttg tgatccagtt cgcccagcac ctggcccgct
    23521 ttgatagcat caccttcgtc caccgccagc gattcaacgc gccccccaac acggaaacta
    23581 agattcaccg tacgaatatc cacgttgcca tacagcgtca ggccgttatc ctggcggctt
    23641 tgataccacc agtagcctcc ggcaaccacg gcggcaagta ccactaccgc cagttcgatc
    23701 acgacaggtt ttttcatcac tacaaactcc tttgcgataa tccttgcaaa atcaggtcga
    23761 tatgacaggt caccgtctgg ttgatcagtt cggtcttttc ttcatcgaac gcggtccagc
    23821 cggtacgtaa cagaattgtt tctttgccaa gacggaacgc cagaatctca ccaatcaacg
    23881 catgggtatg aaggatcatg cgggtgtcat tggcgtcgct gccggtccag gcggcaatca
    23941 ggcgtgtcag gtggctgtgt agcggactaa tcacctgttc gtgcaccagg tggtaggctg
    24001 ccgtgggaga gagctgctca cgggagataa acttgctgag gttgacggtg tcatcctggg
    24061 tgagcagctt aatcatgttc ctgcaggcgc gaaggagcag ttcacggatg gcagcccgat
    24121 caggctgtgg ttgggcgaac aagcgttcgg cttcctcggc atgcggacgg aactgctcgc
    24181 caataaaatc ggcaatccac tgggcgcagg cgaggtacaa atcttctttc gaaccgaagt
    24241 agtaggtgat ggcagcgata ttctgcccgg cttgggcggc tatctcgcgg gtggtggcgt
    24301 tcattccata ttcaccaaac tgcgccagtg cggcagcaat cagctgtttt ttcgcctgtt
    24361 cacccttgat tgtcatggca ggattattca tcgcacagtc cattcttaat caaatgattg
    24421 attaagatta tgactccata tgggagttgt ccagtatggc taagaatttt agctacgcca
    24481 gtcacaggga taatttatgc gctgcgtcac aaaaactgct acactccgct cgctcatgac
    24541 attgtggttt ttgtcatttt ccttttcagt atctccctga aaactacacc ggtaacggtc
    24601 ggggcggttc ggagtagtca tgtctttcga ttatttgggt ttaagccctg atatcctgcg
    24661 cgccgttgcc gagcagggtt accgtgaacc cacccctatt cagcagcagg cgatccctgc
    24721 ggtgctggaa ggccgcgacc tgatggctag cgcccagacc ggcaccggca aaacagcggg
    24781 ctttacgctg ccgctgttgc aacacctgat cactcgccag ccgcacgcca aagggcgtcg
    24841 tccggtacgt gcgctcattc ttaccccgac ccgtgaactg gcggcgcaga ttggcgaaaa
    24901 cgtccgtgat tacagcaaat acctgaacat tcgttcgctg gtggtgtttg gtggtgtgag
    24961 tattaacccg cagatgatga aactgcgtgg cggcgttgat gtgctggtgg caacgccggg
    25021 acgtttgctg gacctggaac atcagaatgc agtgaagctg gatcaggttg aaatcctcgt
    25081 cctcgatgaa gctgaccgca tgctcgacat gggctttatc cacgatatcc gtcgcgtgtt
    25141 aacaaaacta cctgcgaagc gccagaacct gttattctcc gcgaccttct ctgacgatat
    25201 taaagccctg gcggaaaaac tgttgcacaa cccgctggaa atcgaagtgg cacgccgcaa
    25261 taccgcgtct gatcaggtga cccagcacgt tcactttgtc gataagaaac gcaaacgcga
    25321 attgctgtcg cacatgattg ggaaagggaa ctggcagcag gtgctggtgt ttacccgtac
    25381 caaacacggc gctaaccatc tggctgaaca gctcaataaa gatggcatcc gtagtgcggc
    25441 gatccacggc aataaatcgc aaggtgcgcg tactcgtgcg ctggctgatt ttaaatcggg
    25501 cgatattcgt gtactggtgg caactgacat cgctgcgcgc ggcctggata ttgaagagct
    25561 gccgcacgtc gtcaactatg aactgccaaa cgtaccggaa gattatgtcc atcgtatcgg
    25621 gcgtaccggt cgtgcggctg ctaccggtga agcgttgtcg ctggtgtgtg ttgatgaaca
    25681 caaactgctg cgtgatatcg aaaaactgct gaaaaaagag atcccgcgca ttgcgattcc
    25741 gggctatgag ccggacccgt caatcaaagc cgaaccgatc cagaacggtc gccagcaacg
    25801 tggcggcggc ggtcgtgggc aaggtggtgg tcgcggtcaa cagcaaccac gccgtgggga
    25861 aggtggcgca aaatctgcaa gcgcgaaacc tgcagaaaaa ccgtctcgcc gcctcggcga
    25921 tgccaaaccg gcaggcgaac aacaacgtcg ccgccgtccg cgtaaacctg ccgctgcgca
    25981 gtaatctttt atgccgggct atgcccggca tcaggctgat gaacaaacgc aaaactgcct
    26041 gatgcgctac gcttatcagg cctacgtgaa ctctgcaata tattgaattt gcatgctttt
    26101 gtaggccgga taaggcgttt acgccgcatc cggcatttca caacaagcac ttgtcagcaa
    26161 tttgagaaca cgggaaaaaa tttatctgtt ttaccgccca taagccaccg agacgttact
    26221 tctctatagc caattgttct cgcaactcca ttaaaaggta gcccagtcga ttcttgccct
    26281 taccattacc accgtctccc cagtaagcat cgttttccgt atgctcaacc agttttgcgg
    26341 gcgcggttgc cagcaagagc acacgcagtt ctgcatgctg ttcgaattta gcacgaagtg
    26401 ctttacgcat cacctgttct ttaaccgact cccagttttt acgaagtggc ttggaacgat
    26461 tacgccccat gcgagcggcg accatggggg aagaaacccg acggatctct tcgcggtatt
    26521 tttcatcaag gaatttttgt gcctgaaaat agtgttcgga ggtgggccag gttttcccgt
    26581 caaccttgat gggccaggcg gcaaagttag aaaaatcacc gtagtcatcg ctggtgctgt
    26641 agaaatttat gatggtgtct tgcatgacgt gttggatcct ttgtgctcga acgggcatta
    26701 aaccgcatta tgttggtggt tattgcgagc cgctttccag aaacagaaaa accattaccc
    26761 ctgaaaaccg aaaaatgcca caatattggc tgtttataca gtatttcagg ttttctcatg
    26821 gcattaaccg ccgcgcttaa agcgcaaatt gccgcctggt ataaggcgct tcaggaacag
    26881 atccccgact ttattccccg tgtgccgcag cggcagatga ttgcggacgt cgccaaaacg
    26941 ctggccggag aagaaggccg gcatctggcg attgaagccc ccaccggcgt tgggaaaacg
    27001 ctctcctatt tgatccccgg catcgccatt gcccgcgaag agcaaaaaac gctggtggtg
    27061 agtaccgcca acgtggcgtt gcaggatcag atttacagca aagatttacc gctgctgaaa
    27121 aagatcattc ccgatcttaa attcactgcc gctttcgggc gtgggcgcta cgtttgtccg
    27181 cgtaatctga cggcgctcgc cagcactgaa cccacgcaac aggatctgct ggcgtttctt
    27241 gacgacgaac tgacggcaaa taatcaggaa gagcaaaaac gttgtgcgaa gctgaagggc
    27301 gatctcgaca cttatagatg ggatggtctg cgcgatcata cggatatcgt tatagatgat
    27361 gatctctggc gtcgtttgag taccgacaaa gccagctgcc tcaaccgtaa ctgttactac
    27421 tatcgtgaat gcccgttttt tgttgctcgt cgggagattc aggaagcgga agtggtggtg
    27481 gcaaaccatg cgctggtgat ggcggcgatg gaaagcgaag ccgtactgcc tgacccgaaa
    27541 aatctgctgc tggtgctgga cgaaggtcat cacctgccag atgtggcccg ggatgcgctg
    27601 gagatgagcg ctgaaatcac cgcgccctgg tatcgactac agctggactt gttcacgaaa
    27661 ctggtcgcta cctgcatgga gcagtttcgc ccgaagacca tcccaccgct ggcgatccct
    27721 gaacgtttga atgcgcattg tgaagagttg tatgagctta tcgcctcgtt aaacaacatt
    27781 ctcaatctct acatgcctgc cgggcaggag gcagagcacc gttttgcgat gggcgaactg
    27841 cctgatgaag tgctggagat ctgccagcgg ctggcaaaac tcaccgagat gctgcgtggt
    27901 ctggcggagt tatttcttaa cgatttaagt gagaaaaccg gcagccatga cattgtacgc
    27961 ctgcatcggt tgattttgca gatgaaccgc gcgttgggga tgttcgaggc gcaaagcaaa
    28021 ctctggcggc tggcgtctct ggcgcaatct tccggtgcgc cggtgaccaa atgggcgacg
    28081 cgggaagagc gcgaagggca actgcatctc tggtttcact gcgtggggat acgtgtcagc
    28141 gatcagctgg aaaggctgct gtggcgcagt attccgcaca ttattgtcac ctccgcaacc
    28201 ttgcgttcgc tgaatagttt ttcgcgtttg caggagatga gtggtctgaa agagaaagcg
    28261 ggcgaccgtt ttgtggcgct ggattccccc tttaaccact gcgaacaggg caaaattgtt
    28321 attccccgga tgcgcgttga gccttccatc gacaacgaag agcagcatat tgccgaaatg
    28381 gcggcctttt tccgtaagca ggtggagagc aaaaaacatc tcggtatgtt ggtactgttt
    28441 gccagcggac gggcgatgca gcgctttctc gactatgtga cggatttacg tctgatgttg
    28501 ctggttcagg gcgatcagcc gcgttaccgt ttagttgaac tgcaccgcaa acgcgtcgcc
    28561 aacggtgagc gcagcgtgct ggtgggctta cagtcatttg ccgaagggct tgatttgaaa
    28621 ggtgatctgc tcagccaggt gcatatccac aaaatcgctt ttccgcccat cgacagcccc
    28681 gtggtgatca ccgaagggga atggctgaaa agcctcaacc gctatccgtt tgaggtgcaa
    28741 agcctgccga gcgcctcgtt taacctgatt cagcaggttg ggcgactgat tcgaagccac
    28801 ggttgctggg gcgaagtggt tatctacgac aaacgcttgc tgaccaaaaa ttatggcaag
    28861 cgactactgg atgcattacc ggtatttccg atagagcaac cggaagtccc tgaaggtata
    28921 gttaaaaaga aagaaaaaac gaaatcccca cgccgtcggc ggcgttaatg atgtgagtca
    28981 ggtaaggagt cgtaaatgga ctatcgcaaa atcattaaag agatcgggcg cgggaaaaac
    29041 cacgcgcgcg atttagaccg ggatactgcg cgcggtctgt atgctcatat gctcaatggt
    29101 gaagtccctg accttgagtt gggcggcgta ttgattgcgc tgcgtatcaa aggagaaggg
    29161 gaagcagaga tgctcggctt ttacgaagcc atgcaaaatc acaccatcaa gctgacgccg
    29221 ccagcaggca agccgatgcc gattgtcatc cccagttaca acggcgcgcg taaacaggcc
    29281 aacctgacgc cgttgttggc gattctcctg cacaaactcg gttttcctgt ggtggttcac
    29341 ggggttagcg aagatccaac ccgcgtgctg actgaaacca tttttgaatt gatgggtatt
    29401 acgccaacgt tgcacggcgg gcaggcgcag gcgaagctcg acgaacatca accggtgttt
    29461 atgccagtcg gcgcgttttg cccgccgctg gaaaaacaac tggcgatgcg ctggcggatg
    29521 ggcgtgcgta acagtgcaca taccctggcg aaactggcga cgccatttgc cgaaggtgcg
    29581 gcgctgcgtc tttccagcgt ttcgcatccg gaatatattg gacgcgtcgc gaagttcttt
    29641 agcgatatcg gcgggcgggc gctactaatg catggtactg aaggtgaagt gtatgctaat
    29701 ccgcagcgct gcccgcagat caatctcatt gaccgtgaag ggatgcgggt gctgtatgaa
    29761 aaacaggaca ctgctggtag cgagttactg ccacaagcaa aagatccgga aaccacggcg
    29821 cagtggattg agcgttgcct tgctggcagc gaaccgattc ccgaatcgct gaaaatccag
    29881 atggtttgct gcctggtggc tacgggtgaa gcggcaacta tcagcgacgg cctggcgcgc
    29941 gttaatcagg cattttaatt cttttctcag ccggatgagc catgtcatcc ggcttttacc
    30001 ccaccgcaat atgaaattcc tgcatcttta ttgaccttcc cacgcccggc gtgcagcata
    30061 aaaatacaac aaacacataa cataaacagg agttaaccat ggaaagtggt catcgctttg
    30121 atgctcagac gctgcacagt tttattcagg ctgtatttcg tcagatgggt agcgaggaac
    30181 aagaagcgaa attagttgcc gatcatttaa tcgcggcaaa cctggcaggg catgattcac
    30241 atggtattgg catgatccca agctatgtac gctcctggag tcaggggcac ctgcaaatta
    30301 accatcatgc caaaaccgtt aaagaggcgg gggcggcggt cacgctcgat ggcgatcgcg
    30361 catttggtca ggtcgcggca catgaagcga tggcgctggg gattgagaaa gcgcatcagc
    30421 acggtattgc cgccgtggcg ctacataact cgcatcatat cggccgtatc ggttactggg
    30481 cggagcagtg tgcagcggcg gggtttgtct ctatccactt tgttagcgtg gtcggtattc
    30541 caatggtcgc gccgttccac ggtcgcgaca gccgctttgg caccaatccg ttctgtgtgg
    30601 ttttccctcg taaagataat ttcccgctgt tgcttgatta cgccaccagc gccattgcat
    30661 ttggcaaaac ccgcgtcgcc tggcataaag gcgtccccgt gccgccaggt tgcctgattg
    30721 acgttaacgg cgtgccgacg accaatccgg cggtaatgca ggagtcgccg ttgggttcgc
    30781 tgttgacctt tgccgaacat aaaggctacg cccttgcagc gatgtgtgaa attcttggcg
    30841 gggcgctttc cggcggtaaa acgacgcatc aggaaacgtt acaaaccagt cccgatgcca
    30901 ttcttaactg catgaccact atcatcatca acccggaact cttcggcgcg ccggattgta
    30961 acgcgcagac cgaagccttt gccgagtggg tgaaagcctc gccacatgat gatgataagc
    31021 cgattttgct accgggcgag tgggaagtga acacgcgtcg cgaacggcag aagcagggga
    31081 ttccactgga tgcgggaagc tggcaggcca tttgtgatgc agcgcggcag attggtatgc
    31141 cggaagagac gttgcaggct ttctgtcagc agttagccag ctaaaaaaaa gcccgtccag
    31201 tggcggacgg gcaaacaagg gtaacatagg atcaatgagg gttagagcat atgcgtctgt
    31261 cggcaaacag acagggaaat acttgtgctg gacgtagcgt aaacgcctga tccgtccaac
    31321 ggttcggatt ttgtaggcct gataagacgc ggtagcgtcg catcaggcat ctgttgccgg
    31381 atgcggcgta aacgccttat ccgtcctacg aattacttat aaattaccgc agtaccgctt
    31441 aatttattgt tgttggtggc ggaagtgatg ctgtatccac tggcaccggc tgccgcggct
    31501 ttctcagcca gtttcgcttc cagggcatcg agggtggatg cgccatcggc agaaaccacg
    31561 ccgattttat tcatgttctg tgcctgggat gccgttatcg gttccgccgc gaacacgcca
    31621 aatgacagag ttgaaagagc catagcagca acaacagtat tgatagtttt cataattaat
    31681 ctctcgcagg tgatttttgt ataagtgacg ttgtttcgtc gatgtgatga gtatcacgtt
    31741 tttttacgag agataaaatc gaagagaatt gaccgccttg ttcaaataaa ttgattgata
    31801 tctaacatac tgataaacca tttatttgtt gtacttatga acttgtttta taccgcgtgg
    31861 cagtcacagc agcgttaaag gcacggaatg ccacgcaaaa taaaaattat actcgctatg
    31921 catctcgcca gttgtagtta acgtaactgg ctgtctttcg aacctctgcg attatatcct
    31981 gtataagctg gtttttgtaa atctttctcc tgctgacaat gaatacataa gcgcacgcca
    32041 ggaatggctt cccgacgggc ctgcgggatg ggggcaccgc actcttcaca ttcatccagg
    32101 ctttcgccgc gcggaatttc accccgagcg cgggcaatcg catcttcaat tgtactgttg
    32161 atctgttcgt tgacggcgtc atcgttagcc caaccggatg ccatatcgac ctccccatat
    32221 caatacttgt acagttaagt gtagctaatc cagggacgaa ctcgggcagt tcaagcatca
    32281 gatctccgac cattcccgca gcagattatg ataaagatta agcagcgaca ggatctcttc
    32341 actttcgccg tagcggcttt tcagcgactg aatattgttg tccagttcaa acagcatggc
    32401 gcgcttttta tcatcgcgga tcatcgactg gatccacata aatgatgcca ctcgtacgcc
    32461 gcgggttacg ggtggcacgc aatgctggct gctggaggga tacaacacga gatcgcctgc
    32521 cgggagtttt acccgatgtt gtccgaaggt gtcattaacg accagttcgc cgccgtcgta
    32581 gctttgtgga tcgcttaaaa acagcgtggc agaaaggtca gtacgcatcc agccgttttg
    32641 tggatggctg cgtactgcgc catccacatg gaaaccatag gtttcattgt tctgatagcg
    32701 attaaacagc ggcgtggaaa gggtacgcgg caaggccgcg gcaaagaaca aagcatgttg
    32761 gttaaccgcg ttcagcacct cattttgcag ggcggcgtat aacgtgctgc gggtgtcgac
    32821 ctgttgattg ttcttaactt gcgcaccttg tgcgccggtg gtgacgcgtc catccaccca
    32881 ttcggcttgt tccagttgtt cgcgaaaccg ggcgacgtcc tgtggcgata acacgccggg
    32941 aatgtggtac atcatacaag ttctccaaaa agtggggcct gcgccccaca tctgaatcag
    33001 aaatgcatat tggctgtgag caagaaggtt cttggctcgc ccgggtgata acggtagccg
    33061 ctcttgttga ttgaggcgac gtaatcagta tcaaacaggt tgtagacgtt tagctggaag
    33121 tcgagattgc gattaacccg ataccccagt ttggcatcgg cgacccagta accttcggta
    33181 aatgctggcg ttcccaccgc gccgtctgaa cctttatgca tactgccgat atagcgtgcg
    33241 cccgcgccaa cagagatatc gtcggttgcc tgatattggc tccataaggt gaaggcgtgc
    33301 tccggggtat acggcagcga tgaggaacca tcctgggcaa catctttgcc gtttttgatg
    33361 gttgcttttt gctgggtata gccgccaatc acctgccacg cgggagtgat attcccggcc
    33421 acggatatct catagccttc gacgcgtttc ttaccgtatt gcgagtaagt tccgtcatca
    33481 ttttgctcaa cttcattttc gatatcagta cggaacagcg cggcggtgag caacagacgt
    33541 ttatccagaa cctgccattt ggtgccaatc tcgctggtgt tggctttttg cggtttaaaa
    33601 tcggtgcggt tggcactgtt accgctgcca gactgcgcaa gggcgaagtt gttgccgccc
    33661 ggaggctgct gggaaacggc atagttaata tagacattgc cgttttccgt caggtgatac
    33721 agcgccccgg ctttccagtt catcagattg cccgacttgg cggtgtcgac ggtggtgacc
    33781 ggagaacctt ttgccacacc agttgggcag gtgatggcac cgcgtccgct gccgccgcag
    33841 gcggtggcac tgtcatattc agtatgataa ttatccagac ggatcccgcc gttcagctca
    33901 aaatcacggg tgatttgcag cgtatcaaag gcgtaaattg cgaaggtatc cgtctgacca
    33961 ttggcgtttg cgccgttgcg cgtcaggccg ccgggatgaa tgctgctgtc aggatgataa
    34021 atatttaccg cgggtaacgt caccggatta acgccgtagt tagtctgcgt ttcacgggta
    34081 aattccacgc cggtactgac atcatgaccg atagaaccgg tatagaacgt cgaggtcagg
    34141 ttggtctggt tggtgagaat tttattactc acatctttgg tattcgccgt gcgtgaccag
    34201 gttcagctat tcacatcgct ggtgggctga gtaatattcg acgccccgcc cataatcgcc
    34261 gtcatcaggt aatcctgctt tacgcgcgac caacgggtag tattgcgaat ggtggtgtta
    34321 tcgttgatgt cgtgctcaaa acgcatggtg gcggtgtcgg tggtcgaatc gtcgtaatcg
    34381 gaatccgtgc cgtaaaagtt atgagtatca acttttccgg aatgattcag ggccgccgtt
    34441 cccgcagatg gggcagaata gcccggcaaa ccgatggtcg gaatgccgcc gtctggcgtg
    34501 ttgtgctggg tgacatgcag ataattaaga tacaaacgat tcgctgtacc aaggccaaaa
    34561 gcgacagaag gggcgacgcc gtaacgctca tttttgactt tgtcgcgtcc ggcatcgtgc
    34621 gttttttcgc ccattacatt caggcgcacc gcggtggtat caccaatgac ctgattgacg
    34681 tccagcgtgc cgcggcggaa ccaggcgctg ccaatactgg cggaggcgtc aatgccggaa
    34741 tcattgcgcg gctgtttgct gatcatattg atcgagcccg tcggtgcgct gcgcccgtag
    34801 tcggtgccgg acggcccttt aatcacttcg acctgctcgg tattgaaggt gtcgcgcgag
    34861 acgctgccga tatcgcgaat gccatcaata taaatactgt tagaggtatc ggcaccacgc
    34921 atataaatgg cgtcgccagt ggtggagtta ccgttctcac ccgcaaaaaa cgcacccacg
    34981 c
//
LOCUS       DAJTZL010000025        33999 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.025, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000025 DAJTZL010000000
VERSION     DAJTZL010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33999
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.025"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            12..514
                     /locus_tag="OO544_001052"
                     /pseudo
     CDS             12..514
                     /locus_tag="OO544_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
     gene            complement(581..2038)
                     /gene="nuoN"
                     /locus_tag="OO544_001053"
     CDS             complement(581..2038)
                     /gene="nuoN"
                     /locus_tag="OO544_001053"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HCR6766705.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPMGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLYSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSASGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(2045..3574)
                     /gene="nuoM"
                     /locus_tag="OO544_001054"
     CDS             complement(2045..3574)
                     /gene="nuoM"
                     /locus_tag="OO544_001054"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HCR6766706.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRLGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     AEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     EIASQELPGMSLRELFMILLLVVLLVLQGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(3738..5579)
                     /gene="nuoL"
                     /locus_tag="OO544_001055"
     CDS             complement(3738..5579)
                     /gene="nuoL"
                     /locus_tag="OO544_001055"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HCR6766707.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEILHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAIVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLSTWWYNAWGFDWLYDKVFVRPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(5576..5878)
                     /gene="nuoK"
                     /locus_tag="OO544_001056"
     CDS             complement(5576..5878)
                     /gene="nuoK"
                     /locus_tag="OO544_001056"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HCR6766708.1"
                     /translation="MIPLQHGLILAAILFILGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(5875..6429)
                     /gene="nuoJ"
                     /locus_tag="OO544_001057"
     CDS             complement(5875..6429)
                     /gene="nuoJ"
                     /locus_tag="OO544_001057"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HCR6766709.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(6441..6983)
                     /gene="nuoI"
                     /locus_tag="OO544_001058"
     CDS             complement(6441..6983)
                     /gene="nuoI"
                     /locus_tag="OO544_001058"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HCR6766710.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPIYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(6998..7975)
                     /gene="nuoH"
                     /locus_tag="OO544_001059"
     CDS             complement(6998..7975)
                     /gene="nuoH"
                     /locus_tag="OO544_001059"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HCR6766711.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(7972..10698)
                     /gene="nuoG"
                     /locus_tag="OO544_001060"
     CDS             complement(7972..10698)
                     /gene="nuoG"
                     /locus_tag="OO544_001060"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HCR6766712.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     HVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(10751..12088)
                     /gene="nuoF"
                     /locus_tag="OO544_001061"
     CDS             complement(10751..12088)
                     /gene="nuoF"
                     /locus_tag="OO544_001061"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HCR6766713.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(12085..12585)
                     /gene="nuoE"
                     /locus_tag="OO544_001062"
     CDS             complement(12085..12585)
                     /gene="nuoE"
                     /locus_tag="OO544_001062"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HCR6766714.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(12588..14390)
                     /gene="nuoC"
                     /locus_tag="OO544_001063"
     CDS             complement(12588..14390)
                     /gene="nuoC"
                     /locus_tag="OO544_001063"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HCR6766715.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(14484..15146)
                     /gene="nuoB"
                     /locus_tag="OO544_001064"
     CDS             complement(14484..15146)
                     /gene="nuoB"
                     /locus_tag="OO544_001064"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HCR6766716.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(15162..15605)
                     /gene="nuoA"
                     /locus_tag="OO544_001065"
     CDS             complement(15162..15605)
                     /gene="nuoA"
                     /locus_tag="OO544_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HCR6766717.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(16234..17172)
                     /gene="lrhA"
                     /locus_tag="OO544_001066"
     CDS             complement(16234..17172)
                     /gene="lrhA"
                     /locus_tag="OO544_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HCR6766718.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPIEMMSPYLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            18087..19304
                     /gene="alaA"
                     /locus_tag="OO544_001067"
     CDS             18087..19304
                     /gene="alaA"
                     /locus_tag="OO544_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HCR6766719.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            19388..19987
                     /gene="yfbR"
                     /locus_tag="OO544_001068"
     CDS             19388..19987
                     /gene="yfbR"
                     /locus_tag="OO544_001068"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HCR6766720.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVNMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(20046..21878)
                     /locus_tag="OO544_001069"
     CDS             complement(20046..21878)
                     /locus_tag="OO544_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HCR6766721.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(21965..22613)
                     /gene="hxpA"
                     /locus_tag="OO544_001070"
                     /pseudo
     CDS             complement(21965..22613)
                     /gene="hxpA"
                     /locus_tag="OO544_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416796.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
     gene            complement(22624..23118)
                     /locus_tag="OO544_001071"
     CDS             complement(22624..23118)
                     /locus_tag="OO544_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HCR6766722.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(23201..23656)
                     /locus_tag="OO544_001072"
     CDS             complement(23201..23656)
                     /locus_tag="OO544_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HCR6766723.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            23994..25196
                     /gene="ackA"
                     /locus_tag="OO544_001073"
     CDS             23994..25196
                     /gene="ackA"
                     /locus_tag="OO544_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HCR6766724.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            25271..27415
                     /gene="pta"
                     /locus_tag="OO544_001074"
     CDS             25271..27415
                     /gene="pta"
                     /locus_tag="OO544_001074"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HCR6766725.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            27605..29125
                     /gene="yfcC"
                     /locus_tag="OO544_001075"
     CDS             27605..29125
                     /gene="yfcC"
                     /locus_tag="OO544_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HCR6766726.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATMIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGTRMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(29158..29700)
                     /gene="yfcD"
                     /locus_tag="OO544_001076"
     CDS             complement(29158..29700)
                     /gene="yfcD"
                     /locus_tag="OO544_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HCR6766727.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(29758..30309)
                     /gene="yfcE"
                     /locus_tag="OO544_001077"
     CDS             complement(29758..30309)
                     /gene="yfcE"
                     /locus_tag="OO544_001077"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HCR6766728.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(30365..31009)
                     /gene="yfcF"
                     /locus_tag="OO544_001078"
     CDS             complement(30365..31009)
                     /gene="yfcF"
                     /locus_tag="OO544_001078"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HCR6766729.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLKPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RRIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            31145..31792
                     /gene="yfcG"
                     /locus_tag="OO544_001079"
     CDS             31145..31792
                     /gene="yfcG"
                     /locus_tag="OO544_001079"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HCR6766730.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLTEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            31849..32211
                     /gene="folX"
                     /locus_tag="OO544_001080"
     CDS             31849..32211
                     /gene="folX"
                     /locus_tag="OO544_001080"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HCR6766731.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            32232..33125
                     /locus_tag="OO544_001081"
     CDS             32232..33125
                     /locus_tag="OO544_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HCR6766732.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVFISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNDLRG
                     PFNMVSPYPVRNEQFAHALGQALHRPAILRVPATAIRLLMGESSILVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(33173..>33999)
                     /gene="rpnB"
                     /locus_tag="OO544_001082"
     CDS             complement(33173..>33999)
                     /gene="rpnB"
                     /locus_tag="OO544_001082"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HCR6766733.1"
                     /translation="TARDFIDIHLPAPLRKLCDLTTLKLESNSFIDEDLRQYYSDLLW
                     SVKTQEGVGYIYVVIEHQSKLEELMAFRMMRYSIAAMQNHLDAGYKELPLVIPMLFYH
                     GCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDITVVPDDEIMQHRKMALLELIQKH
                     IRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQTGDAQRFRAFIGEITERAPQEK
                     EKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLDRELVMMVTRLSPDDLIAQSH"
BASE COUNT         8480 a         9277 c         8564 g         7678 t
ORIGIN      
        1 aggaaaaata aatgggaatg attggttatt ttgcagaaat tgactcagaa aagattaacc
       61 aactgcttga gtctactgaa aaacctttga tggataatat acatgacacg ctttcaggat
      121 tacgccgact ggatattgat aaacgctggg attttttaca ctttgggctg acaggcaccc
      181 ctgcttttga tcccgcgaag aatgatccgc tgagtcgtgc cgtgcttggt gaacacagcc
      241 tcgaagatgg catagatggc ttcctgggat tgacctggaa tcaggagtta gccgcgacaa
      301 tcgaccgact ggaatcactg gatcgaagtg agctgcgtaa acagttttcc attaagcgac
      361 tcaatgaaat ggagatttat ccgggagtga ccttttcaga agagttagag gggcaactct
      421 ttgcttcgat catgctgata tggaaaagct gattagcgcg tatcgacgta tgctgcgaca
      481 agggaatcat gcgctgacgg ttattgttgg gtgatggtgt cagggcacca aaaaaaaccg
      541 ccgaacatgt cggcggcttt ctgacttaca aactgatagc ttacatcagc ggcattgcta
      601 accgcacaat gctaatcagc ggttgtggcc atacacccag caccagtacc aacagtgcag
      661 agatcagcac cacgataccg cctgcgctgt actgccagtt tgacggtgca tcgcgacccg
      721 gttgttccgg ggcgtgcagg tacaggctca ccgccacgcg caggtagtag taaaggccgc
      781 ttgccgaacc gacaaccacg gcgcccacca gccaccacaa gtgtgcctgg acaccgaccg
      841 ccagcacgta gaacttacca ataaagccca gcgtcatcgg aataccggcc agagacagca
      901 tcatcaccgt catcaccgcc gcgaggatcg ggcgatgcca gaacagtccg cggtaagagt
      961 acagggaatc agcatccggg ccacgatacg ggctggacat caggctgacc acgccgaacg
     1021 cgccgaggct gctaaacaga taaccggcca ggtaaacccc taccgcttcc atcgacatct
     1081 cgccagtttg cagcgcaatc agcgccacca gcagatagcc gaggtgagag atagatgagt
     1141 aaccgagcag acgtttgata ttggtctggc tcagcgccat caggttaccg aagatgatgg
     1201 aggcaaaggc gataatcgcc agcaccacgc gaatcgcttc gctgtcaccc atcggtgcgt
     1261 agaggaacag acgcatcacc acaccgaaga tagcgatttt gctcgccgtc gccaagaaag
     1321 tggaaaccgg cgcaggcgcg ccctggtata cgtctggcgt ccacaggtgg aacggcacca
     1381 gagagagttt gaagccgagg ccaacaatca tcaggccgaa acctgccagc aacagcggct
     1441 cgttgagcat accgtcgcca aggtttttac ccaacgcgac aaacgacagg tcgccagact
     1501 gcgcatacac cagcgccata ccaaacagca ggaaagaaga cgctgcggca gaaaggatgg
     1561 tgtatttgat actggcttcc agtgaacgtt tctggcggaa agcgtaaccg accaggccaa
     1621 acagcggcaa agagatcagt tcgataccga ggaacagaga cgccagatgg ttggcattcg
     1681 ccagcaggat cccgcccagc gcggcaatta acaccaacag gtagaactcg tccttgttgt
     1741 cgttatagcc ttcaagccac gggtaggcga aagtacaggt ggcgaggctc gccaacaata
     1801 ccagcccggt gtaaagcatg gcgaaaccat caacgcgcat cagcggcgta acgtccatag
     1861 cgcctgcctg gccgacaaac cagagcgaaa ccagcgccgc gttaagccca ataaccgaga
     1921 gcgtagcgtt gaggaaatga ttgcgtcgcc acgcaatgga gagcatcaca accaccaccg
     1981 tcaagccgac gatcagcaac ggtagcagtg cgatcaggtt ttgtggagtt attgtcatgg
     2041 cgatttacgg ccttgtagta gtaacggaat taacaaacca ctgctggata ttgccaatcg
     2101 cggagtgcga ggtatccaga atcggctgcg gatagaagcc ctgcagtacc agcagcacca
     2161 ccagcaacag gatcataaac agctcacgca gcgacatccc tggcagttcc tggctggcaa
     2221 tctcgctttt cgctttaccg aagtaagcgc gatgcaacat cgccagcgaa taaacagatg
     2281 caaagaccag cccaaaggta gagataacgg taatcaccgg gacaacctgg aagctgccga
     2341 acagaatcat aaattcgccg acgaagttac cggtgccagg catcccaagc gttgccaccg
     2401 caaagaacag cgacagtgct ggcagccatt tcatcttgct ccacagaccg cccatcatgc
     2461 gcatgtcgcg ggtatggata cgttcataaa gctgaccaca aagaataaac agacccgccg
     2521 ccgacaagcc gtgcgcaatc atctggatta ccgcgccctg gtaggccaac tggctgccgg
     2581 tgtagatagc aatcagcacg aagcccatgt gggaaaccga ggtgtaggcg atcagacgtt
     2641 tgatatcggt ctgggcgaag gccatccacg caccgtagaa gatgccgata acacccagcc
     2701 acatagcaat tggcgcgaac tctgccgacg cgttcgggaa cagcggcagg gagaaacgca
     2761 gcaaaccgta agcggcagtt ttcagcaaga tccccgcgag gtcaacggaa ccggcggtcg
     2821 gagcctggga gtgcgcatcc ggcagccagc catgcagcgg aaccaccggc attttgactg
     2881 cgaaggcgat gaagaagccc agcattaaca ggtattccgc accactggac attggcgtat
     2941 tcagcagctc ttcatagttg aaggtccaga cgccggtcgc attgtagtga acaaaaacca
     3001 gcgccaggat ggcaatcaac atcaccagac cactcgcctg ggtgtaaatg aagaacttgg
     3061 ttgccgccgt gatacgcgtt ttaccgtcag aggctttatg cccccacagt gcgatcagga
     3121 agtacatcgg caccagcatc atttcccaga agaagaagaa caggaacatg tcgatggcaa
     3181 ggaacacgcc gataacgccg cccaggatcc acatcaggtt gaggtggaag aagccctgat
     3241 atttttcgat ctctttccac gaacatagta ccgccagcac accgagcaga ccggtcagca
     3301 cgaccatcag cagcgacagc ccgtcaatgg cgagatgaat agagataccc aaacgcggga
     3361 tccacggcat gtcgaattca gactgccact gcggaattcc ggcggattgc gtcagtgaat
     3421 aaccgccctg caaccacagt tgcagcgaca gcgccagcgt caatcccatg gtgatcagcg
     3481 cgatccagcg tggcaccttg acgccaaagc gttcggtctg ccagcacagg aagccgccaa
     3541 taaagggaat taatattagc cagggtagta acatggcgat ctttattcct tgtaaaagtc
     3601 ccgtcaggac cggattttca acgaattctc acgacagaaa attctcttcg gactgggggt
     3661 agtgtcggat gcaccgcgaa ggcggcttat ccgacctacg ttcttatatg cccaggggca
     3721 atcccacaat ccttaactca acgcagtacc atcaacagtg ccagcaccac gaccgcaccg
     3781 atgctcatgg atgccacata ccagcgcaga tagccgttct cacttaacag cagaccttta
     3841 cctgcaaagc gggaaaggac agccggaatg ttcatcattg agttcagcgg atcgcgtttc
     3901 agcaaccagg caatacccag gaacggcctg acgaacactt tgtcgtacag ccagtcaaag
     3961 ccccaggcgt tgtaccacca ggtactcagc aggcggcccg gcgcactgtt ggcgatggag
     4021 gtcaccagag tacgtttacc cagccacagc caagctgcca gcagaatgcc gacaatcgcg
     4081 accacgccag aggtaatttc cagggtcaac atgctgccgt gcgccagttc cgtcgtttgc
     4141 ggaagcacgc cctgcagcgg cggtacaatc agtgcgccaa cgaaggtgga aaggatcagc
     4201 agcacaatca gcggcaggct gtgagttacc cctttcacgg cgtgagcgtg aatttgttct
     4261 tttccgtgga agacgatgaa aatcatacgg aaggtgtaga gcgaggtcat aaacgcaccg
     4321 accagacctg ccaccatcag attgatatga ccattcgcca tcgcacccgc gaggatctca
     4381 tccttactga agaagcccgc agtgaccagc ggcagtgccg acagtgctgc gccgcccacc
     4441 aggaagcaga gataaaccag cggaatagat ttacgcagac cgcccatctt gaagatgttc
     4501 tgttcgtgat ggcaggccag aatgacggaa ccggatgcca ggaacagcag cgctttaaag
     4561 aacgcgtgag tcatcaagtg gaaaatcgcc gcatcccatg cctgcacgcc aagcgcgagg
     4621 aacatgtagc caatctggct catggtagag taagcgagaa cacgtttgat gtcggtctgt
     4681 accagcgcgg caaaaccggc cagcagcagc gtaaccgccc cgacaatgcc caccagatgc
     4741 agaatttccg gcgtcatcag gaacaggccg tgggtacggg cgatcaggta gacacccgcg
     4801 gttaccatgg ttgcggcgtg gatcagcgcg gagacaggcg tcgggcccgc catcgcgtcg
     4861 gcaagccatg tctgcaacgg caactgcgca gatttaccga ccgcaccgcc cagcagcatc
     4921 agcgtcgccc acatcagcat gttattgccg tcagcaaagt gcgctggtgc cagttccacc
     4981 atttcgcgga agttcagggt gcccagttcg ttgtaaagaa tgaacagtgc gaaagcgagg
     5041 aaaacgtcac ccacacgggt cacgacgaac gctttcattg ccgctgcgcc attcttcgga
     5101 tcggtgtaat agaaaccgat cagcagatag gagcacaggc ccacgccttc ccagccgagg
     5161 tacatcagca gcaggttgtc ggcaagcacc agaactacca tgctggcgat gaacaggttg
     5221 gtgtaggcga agaagcgaga gtagccctct tcaccgcgca tgtaccagga ggcgtacatg
     5281 tgaataagga aacccacacc agtgaccacc gagagcatgg tcagcgacag gccgtccagc
     5341 accaggttaa aaccgatgtt aaagtcgcct accgacatcc acgtccacag cggctggctg
     5401 tatgcctgct cgccgttagc gaagaaatca acgccgataa aggcggttac cagcgccgcc
     5461 aggcccacag agccaacgcc gacgatcgcc gaaacgtttt cagaccagcg cccacgggag
     5521 aacgccagca ggacgaagcc aatcaatggc aaaataatgg ttaaggcaag catgttcatc
     5581 cgcgcatctc acttactgaa tcgatgttca ggttctggcg acgacggtga agttgcagca
     5641 gcagcgcaag gccgatactc gcttctgccg ccgcgaggct gatggcgaga atgtacatca
     5701 cctgaccgtc ggtctgaccc cagtagcttc cggcgacaac aaaggccagc gcggaggcgt
     5761 taatcatgat ttccagacca atcagcataa acagcagatt gcgacggata accagaccgg
     5821 ttaagccaag aatgaataag attgccgcga ggatcagtcc atgttgtaag gggatcatgc
     5881 gtgctcctcc gtttttcttt tcgcgctgtc gtctttacga ttgctcagca cttcacccgc
     5941 acgctcttca cgaccgacgt ggaaggccac aaccagacct gcgagcagca gcatagaagc
     6001 cagttccacc gccagtacgt aaggcccgaa cagcgtaata cccactgctt tagcactgat
     6061 tggcgtaccg tcgataccct gatcgttaac accgaggatg gcgtaaacaa tcaccaccag
     6121 catgatggcc gacaaaattg ccggaccaat ccacacctgc ggtttcagcc actggcgttc
     6181 ctgttcgatt tctgaaccgc ccaggttgag catcatcacc acgaacacga acagcaccat
     6241 aatggcaccc gcgtagacga taatttccag cgcaccggcg aagtaagcgc ccagtgagaa
     6301 gaacaccccg gaaatcgcca gcagcgaaat aatcaggtac agcagtgcgt gtaccggatt
     6361 ggtatgggtg atcactcgca aggtcgcaag tatggctatc aggccacaga tataaaaagc
     6421 gaactccatt gcccctctcc ttacggtaac aggctcttga cgtcgatagg cttggcttcg
     6481 ttctctgctt cgcccttatc tttgccgtcg attgccatac ctgccatccg gtagaagtta
     6541 tattccgggt atttgcccgg accggagatc agcagatcct ctttttcgta aaccagatcc
     6601 tggcgcttgt attcccccat ttcgaaatcc ggggttaact gaatcgccgt ggtcggacag
     6661 gcttcttcgc acagaccaca gaaaatgcag cgtgagaagt tgatgcggaa aaattccgga
     6721 taccagcgac cgtctttggt ttctgctttt tgcagcgaga tacagccgac cgggcaggct
     6781 accgcgcaga ggttacaggc tacgcaacgc tcttcgccgt ccgggtcgcg ggtcagaacg
     6841 atacgaccac gataacgggg cggcagatag atcggctctt ccgggtacat tcgcgtttcg
     6901 cgtttggcga acgcgtgcag gccgatcatc cagatactac gaacctgggt gccgaaacct
     6961 actaacaatt cttttaaggt catggtctta ttgcccctta ttgcgcctgc cagagaatga
     7021 cagccgccgt taccagcaag ttgatcagcg tcagcggcag gcagattttc cagccgaagg
     7081 acattacctg gtcataacgc ggacgcggta acgacgcacg aatcaaaatg aacatcatca
     7141 taaagaacgc ggttttcagc gcgaaccaga tgaatggcgg taacaacggg ccttgccagc
     7201 caccgaagaa cagcgtcacc atcaatgcag agatggtcac aatcccgatg tactcaccca
     7261 cgaagaacag accgaacttc ataccggaat attcaatgtg gtaaccatcc gccagttcct
     7321 gctcggcttc cggctggtca aacgggtgac ggtgacatac cgccacgccc gcgatggcaa
     7381 aggtaataaa accaaagaat tgcgggataa cgttccacac atgcgcctgg ctgttgacga
     7441 tgtcggtcat gttgaatgaa ccggcctgcg ccaccacgcc catcagggaa agcccgagga
     7501 acacttcgta gctcagggtc tgcgcagaag cacgcatcgc acccagcaac gagtatttgt
     7561 tgttacttga ccagcccgca aacagcaccg cgtaaaccgc cagacctgcc atcatcagga
     7621 agaacaaaat cccgatgttc aggtcggcaa ccacccaacc cggactgact ggcacaatcg
     7681 caaaggccag cagcagcgag gtaaaggcaa tcatcggtgc cagggtaaag atgacgcgat
     7741 ccgagaattt cgggatccag tcttctttaa agaacatttt gatcatgtcc gcaaccagct
     7801 ggagcgaacc gccccagcca acacggttag gtccgtaacg gttctggaac agacccagca
     7861 ggcgacgttc gccaaagctc atgaatgccc cgcaggtgac aaccaccagc aggatcacca
     7921 ccgctttgag gatggtcagc aggatctcaa tcagttccgg tgatatccaa ctcattgttg
     7981 tgcctccttg agatcctcaa gatgcgcgcc agccagcacc ggagcaatac cggacatacc
     8041 catcggcaag cccacctgcc ctgccgtcag cccttcagcg atttcaaccg gcagcgtgac
     8101 cgtattgcca tcgtaactaa aggagacgtg tgtacctgcg ttcacaccta acttcgcggc
     8161 atccgctggg ttgagtttga tgtacggctg cggcatacgg ctctggaaga ccggagcacg
     8221 ctgtgacaat tcatcgctgc caaacaggtg gtaatacggc gcgatacgcc atttcccgtc
     8281 ctgcggctgg aagcgtgccg gtacgctggt gaagtaatcc agaccatttt cgctggtttc
     8341 aaacagacgc acgcccggat cgccaaagcg cagtttgccg cccacttcgt cctggaattt
     8401 gttccacgcc tgcggggagt tccagcccgg cgcccaggca aacggcactt gcgaacggtg
     8461 cgcagtcggc tggttgttac cttccatcga gaaggtgaac atggtgtcaa tatcctgcgg
     8521 ctgacgcggc tcatgaacgc tgatattggc gcgcatggcg gtacgaccgc tgtaacggtg
     8581 cggttcacgg gccagtttct gcccacgaat acggaatgtc gcatccggcg cagcatcttt
     8641 gatacctgcc agttccggga ttttcgccac aacagcgtca atcacatggt cgagctgcgt
     8701 ccagtccact tcacggctca gcagggtgct gtgcagcgag tgtaaccagc gccagctttc
     8761 cagcatgaca gttttgctgt cgtaataagc aggatcgtaa acctggaaga aacgttgggc
     8821 gcggccttcg ttgttgatca ccgtaccgtc gctttcagca aaactggcag cagaaagtac
     8881 cagatgggcg ttttccataa tcgctgtgcg ttgatgatca accaccatca ccagcggcgc
     8941 tttagccagc gcagcattca cgcgggtagc ggaagcgtga cgatgcagat cgttttccag
     9001 caccaccacc gcgtcggcgc gtccggtttc cagttcggtc aacgcttctt caagcgaacc
     9061 gccacccata atgcccagcc ccatgctgtt gacggaacgg gcaatcatgg tgataccaac
     9121 gtcagcgccg cgacctttca gggctttcgc gacgttagcc gcagcctgaa tcacctctat
     9181 gctaccggcg ttcgtcccgg agataatcaa cggtttcttc gcacctgcca gtgcctgcac
     9241 gatgacgtcg attttgcttt gcagctcagg ttcgataccg tcaaccgctg gcgcagagtt
     9301 atccagcgca tgggcgatgg caaaacctaa acgcgcctga tcttcaaccg gtgcgcggta
     9361 agtccacgcc gcgatatcat ccagacgggt gtcatcaacg ttggtaacaa acagcggatg
     9421 cttcgcacgt tgaccgatgt tgaggattgc cgcaatctgc cagtcagcca ctttctgtgc
     9481 tgccgccatt tcgcgcgctt tacctttcac agcctgacgc actgccagcg cgacgcgcgc
     9541 gccggtctgg gtaacgtctt cgcccagcac cagtaccgca tcgtaagatt cgatttcgcg
     9601 cagagccgga gtataaatgc cgccttcacg cagcactttc agcgccagtt gcagacgttc
     9661 ctgctcaccg tgagcgatac cggtgtagaa gttttcttcg cccaccagtt cacgcagcgc
     9721 aaagttgctt tccacgctgg cacgcggaga accaataccg atcactttct tcgactgacg
     9781 cagaatatct gccgcgccct gcattgcctg ttcggcgttg agggtaatga aatcatcgcc
     9841 acgacgctgt actggctgac gcggacgatc cttcaggttg acgtaaccgt aaccgaaacg
     9901 accacggtcg cagaggaagt agtggtttac cgtaccgttg taacggtttt cgatacgacg
     9961 cagttcgccg taacgttcac cggggctgat gttacagccg atggaacatt gctggcagat
    10021 gctcggcgca aactgcatat cccatttacg gttgtaacgc tcggagtgcg ttttgtcggt
    10081 aaatacgccg gtcgggcaaa tttcgaccag gttaccagaa aattcgcttt ccagcgtgcc
    10141 gtcttccggg cgaccgaagt agacgttgtc gtgcgcaccg taaacgccca gatctgtacc
    10201 gtcagcgtaa tctttgtagt aacgcacaca gcggtagcag gcgatgcagc ggttcatttc
    10261 gtgagagatg aatggcccca aatcctgatt acggtgggta cgtttggtga aacggtagcg
    10321 acggaagctg tgtccggtca tcacagtcat atcctgaaga tggcagttac cgccttcttc
    10381 acataccgga cagtcgtgcg ggtggttggt cattaaccac tcgaccacac tttcacggaa
    10441 ctgtttcgct tcttcgtcgt caatggaaat aaaggtgcca tcggaagccg gtgtcataca
    10501 ggacatcacc aggcgaccac gcgtgtcttc cgcgttttgg tattgcttca ccgcacactg
    10561 gcggcaagca ccgacgcttc ccagcgccgg atgccagcaa aagtaaggaa tatcaaggcc
    10621 cagagacaga caagcttcca gcaggttgtc cgctccgttg acctcgtatt ctttgccgtc
    10681 tacatgaatt gtagccatta gcatgcttcc agttttctcg gtcagggacc gagcgttaat
    10741 cgaaattcgg ttaccagcgc tctttcagca ggttcggctg aatcccatta atcaaatggg
    10801 tattgctgaa cggctgtttg attcccgcct caaattcttc gcggaaatat ttgatggcgc
    10861 tctgtaacgg ctccactgca ccaggtgcgt gggcacagaa agttttaccc gggcctaaga
    10921 atcgacacag ttgctcaagt gtttcgatat cgcccggctg gccttcgcca cgctccagcg
    10981 cacgcagaat tttcacgctc cacggcagac cgtcgcggca cggcgtacac cagccgcagg
    11041 actcacgggc gaaaaactct tccaggttac gcaccagcga caccatgttg atctcatggt
    11101 caaccgccat cgccagcgcc gtacccagac ggctgcccgc tttaccgata ctttcgaatt
    11161 ccatcggcag atcaaggtgc gcttcggtca ggaagtcagt ccccgcgccg cctggctgcc
    11221 aagctttaaa tttcagacca tcacgcatac caccggcgta atcttcgagg atctcgcgtg
    11281 cggtggtgcc gaacggcagt tcccacagtc ccggattttt cacccgaccg gagaagccca
    11341 tcagcttggt gccagcatct ttacttttcg agatgttctg ataccactcc acgccgttag
    11401 cgaggatcgc cggaacgtta cacagggttt cgacgttgtt gacacaggtc ggtttgcccc
    11461 atacgccgga ggttgccggg aaggggggtt tcgagcgtgg gttagcacga cgcccttcca
    11521 gggagttgat taacgctgtt tcttccccgc agatgtagcg ccctgccccg gtatggacga
    11581 acagttcgaa gtcgaaacct gttcccataa tgtttttgcc aagcaagccc gcttcagtgg
    11641 cttcggcaat ggcgcggcgc agatttactg ccgcttcgat atattcgcca cgcaggaaga
    11701 tgtagccacg gtaagctttc agcgcaaacg cggagatgag cataccttcc accagcaggt
    11761 gcggcagttg ctccatcaac aggcggtctt tataggtgcc cggctccatt tcatcggcgt
    11821 tacacagcag gtaacggatg ttcatggatt cgtctttcgg catcaggctc cacttcaggc
    11881 cagtcgaaaa gcccgctccg ccgcgccctt tcagaccagc gtcttttacc tgattaacga
    11941 tttcgtccgg agacagcccg gtcagcgcct tacgcgcccc ttcgtaaccg tttttgctgc
    12001 ggtattcgtc cagccacact ggctgtttgt catcgcgcag acgccaggtc agcggatgcg
    12061 tttcgggagt acggataatg tttttcattt ataccgctcc agcagttcag ggatcgcttc
    12121 cggggtcaga tgcgcgtgag tgtcctcatc gatcatcatg tttggccctt tatcacagtt
    12181 ccccaggcag caagttggca gcagcgtaaa gcggccatca aatgtcgttt gccctggttt
    12241 gatgttcagc tttttctcga gcgccgcctg aataccctga tacccgttga tatgacagac
    12301 cacgctgtcg caataacgga tcacatggcg accaaccggc tggcggaaga tctgactgta
    12361 gaacgttgcc acaccttcga cgtcgcttgc cggaataccc agtacatcgg cgatcgcgtg
    12421 gatcgcacca tccggcaccc agccacgctg cttctgaacg attttcagcg cttcaatgga
    12481 cgccgcacgc gggtcttcgt agtggtgcat ctcgtgctca atcgcttcac gctctgccgc
    12541 actcagctca aaagcctcgg tttgtggttg ttgattctcg tgcataatta gcggtccaca
    12601 tctgacataa caaaatcgat actgcccaga taaacaatca ggtcagacac caggctgccg
    12661 cggatagccg ccggaatttg ctgcaaatgc gcataactcg gcgtgcggat acgagtgcga
    12721 taactcatgg tgctaccgtc gctggtcagg tagtaactgt tgatcccctt ggtcgcctca
    12781 atcatctgga aagattcatt ggcaggcatc accggccccc acgacacttg caggaagtgg
    12841 gtgatcaggg tttcgatatg ttgcagcgtg cgctctttcg gcggcggcgt ggtcagcggg
    12901 tgatccgctt tgaacgggcc ttccggcatg ttgttgaggc actgctcaag aatgcgcaga
    12961 ctctggcgca gctcttccac tttcagcatt acgcgggtgt agcagtcaga aacaccacct
    13021 ccaaccggga tttcaaagtc gaagttttca tagccagaat aaggacgcgc cttacgcacg
    13081 tcgaagtcga tcccggtagc acgcaggccc gcgccagtgg tgccccactc cagcgcctct
    13141 ttcgcgccat aggcggcaac gccctgggaa cgacctttca gaatggtatt ttgcagcgcc
    13201 gctttctcgt aagacgccag acgtttcggc atccagtcga ggaactcacg cagcaggcga
    13261 tcccagccgc gcggcaggtc gtgcgctacg ccgccaatac ggaaccacgc cgggtgcata
    13321 cggaaacccg tgatcgcttc caccagatcg taaattttct gacgatcggt aaaggcgaag
    13381 aacacgggcg tcattgcgcc gacgtcctga ataaaggtcg agatgtacag caggtggctg
    13441 ttgatacgga acagttcgga gagcataacg cgaatgacgt taacgcgatc cggcacggtg
    13501 atcccggcca gtttctctac cgccagcacg taaggcattt cgttaacgca gccgccgagg
    13561 tattcaatac ggtcggtata cggaatgtag ctgtgccagg actggcgctc gcccattttc
    13621 tccgcaccac ggtggtggta gccgatgtct ggtacgcagt cgacaatctc ttcgccgtcg
    13681 agttgtaaaa cgatacggaa agccccgtgc gccgacgggt ggttcggacc gaggttgagg
    13741 aacatgaagt cctcgttttc ggtgccgcgc ttcatccccc actcttccgg tttgaaggtc
    13801 agggcttcca tctccagatc ctgtttggct ttggtcagct caaacggcga gaattcggta
    13861 gcgcgcgccg ggtaatcttt acgcagcggg tgacctttcc aggtttgcgg catcatgatg
    13921 cggcgcaggt tcgggtgacc gtcgaaagta atgccaaaca gatcccaggt ttcacgctca
    13981 taccagttag cgttcgggaa cagtttggtg aaggtcggta cgtgcaggtc gttttctgcc
    14041 agcgccacct tcagcatgat gtcgcggtta cgatcgatag aaatcagatg gtagaaaacg
    14101 gaaaaatccg cggcaggtaa cccttcgcgg tgtgtgcgca gacgttcgtc catgccgtgt
    14161 aagtcaaaca gcatgacgta aggtttcggc agtttcttta agaaatcgcc aacttccagt
    14221 aattgttcac gcttgatcca cacaacggga accccggtgc gagtcgcctg aacagtaaag
    14281 gcatccggcc caaaacggtt gcgcagttcg ccaatcaccg gatcatcaag atgatcacgg
    14341 gtctgccagg cgggttcttg cgcggttaag tcggtcatat tgttcaccat tgcaaatggt
    14401 ccgtggtgac tgtcggcgca gtaagcttcg cgaaatagat ttaggaataa gcgaagaaaa
    14461 atccctttgc cgacaggcgc aaattaaatc tcgtcaggtg tacgcaggtt ggttacggca
    14521 atgcgttcac cgcgcttgcg ttcgcgctct gattgcatat tggcgcgata aacgccctga
    14581 tcgccaacca cccaggagag cggacgacgt tctttgccga tagattcctg caacagcatc
    14641 agtgcctgca tgtacgcttc aggacgcggc gggcagcccg ggatatacac atcaaccggg
    14701 atgaatttat cgacgccctg cacaacggaa taaatatcgt acataccacc agagttggca
    14761 caggcaccca ttgagataac ccattttggt tccagcatct ggtcatacag acgctgaata
    14821 accggtgcca ttttggtaaa gcaggttcct gcaaccacca tcaggtcagc ctgacgcggc
    14881 gaagcacgca atacttctgc gccaaaacgc gccacgtcat gcaccgcggt aaacgaagtc
    14941 accatctcaa cgtaacagca ggaaagaccg aagttatacg gccaaattga gtttttacga
    15001 ccccagttaa ccatgtcatt gagcttgccc ataaacacgt ttttgttaac ttcttgctcc
    15061 agagggtcgg ttacgatctc ctgcttttgc agggggtaac ggtcgttctc accgttggga
    15121 tctatgcggg tgagcgtata atccatctta atgcctcgcg gttagcgttg acgattagcg
    15181 atactgttcg tttccgggtt catacgctcg cggcgtgaac gcgcgggcgt ccagtccagc
    15241 gcgccaatac gcaccagata aaccagacct gccagtaaca caaaaataaa aattgcagct
    15301 tccacaaagc ctacccagcc gctttcgcgg atagaggttg accatgcgaa cagatacagc
    15361 gcttcaacgt cgaagataac gaagaacatg gccaccagat aaaacttggc ggacaggcgt
    15421 aagcgggcgg agccgaccga gtcgataccg gattcaaacg gcacgttttt cgacctcgcg
    15481 cgtgcgcgac cgcctaaaaa ccaaccgcct accagcatca ggcaacacag gccaatggca
    15541 acgataagaa agatagcgaa tgcccagtga tgagcgatga cttcagtgga tgttgacata
    15601 ctcattgctt actcatcaaa agtagcgcca gattcactgc tcttcacggc agatggacgc
    15661 cacatcgatt catggggagg aataaaaaaa accttacaat cactgtagaa attcttttat
    15721 acagctaatt gatgtggtct tttactcctt tctataacct tttgtcaact ttaacaaaag
    15781 tttcttcaca ttagtttaca taatatcaac accattagca tttaatgccc tttcacgcca
    15841 gatccttgac gactccagga taattagatg ttgttgaatc gtgtccgttg tgaagcaatg
    15901 gaaaaaatac gggtctattt tgacaggaat ttgtgtcgat tcctcccccc aaaagagagt
    15961 attttcttga tctgtgacac gcttttgtca ttccataaca aaaacgcagc aacaaattta
    16021 cgcattttta acatcattgt agcaggtgat ttttttcagg cgattatttg tgcgttcggg
    16081 acgtgaatct ctggtgattg aaaaatgaac agttttgtac gttctgcact atgcgaggaa
    16141 ggatttttac taaaaaaaag ccgctggggt ttaaaacacc cccagcggct cgtttttaca
    16201 ctattgtctc aggaattatc tatcgtccgt cgattactcg atatcccttt caatcaacaa
    16261 tgaatcatcc ccttccggag cagacattgg gctgtattgc cacggattat ggtagctctc
    16321 catcgcctga tagatcacct gggccagctc attattactg gacgggtcat agcacagcaa
    16381 atattcggta tcaggtagcg gcggtaagcc atctactcca ctcagcacac gcaggtacgg
    16441 gctcatcatt tcaatcggcc ttgccgtcac gccaagaccg gctttcactg ccgcacgaac
    16501 ggccggaagc gtcgaggcga cataagccag tcgccatgga atatctgctt tattaagcgt
    16561 cgccagcacc atatcgcgaa acggacttgg atcatccagc aatacaagag ggatcggctc
    16621 gcctttttgc aatacgtatt ccgctgcgca gtaccagtgt gttggcgagg tacgcaggtt
    16681 caatgcctta aacgctgaag gacgatgggt ggttaccatc aaatccactt cctgggattc
    16741 cagcatttct gccatatagg cattacgttt aacgcggaca tccagcgcta atttcggata
    16801 aaccgaactc acgcgattta acaggaaagg taagatcgta tcggcagatt catctgaagc
    16861 accgatagtt aacacgccct gaagattact gaacattaat gatgagcagg cctcatcatt
    16921 aaaacgcagt attttcctgg cgtaaccaag aagttgaatg ccatgttcag ttaacagttt
    16981 gttgcgaccg tgacgagcga acagttcttt cccaacgagt tgttccagac gctgcatttg
    17041 ctgacttacg gcggactgag tacgacacac agcggcagct gcggcagcaa aagtgttcag
    17101 atcggcaaca gcaacaaatg ttctcagcag atcgaggtcg aggttaatta tcggacgatt
    17161 tgcacttatc atatattatc acttactggc ggctcatact gagctggtta atgctgtgca
    17221 cacacaaaca agcaattcca tttgtaatgt gcctccctgg cagtttcatc cggaacccgg
    17281 acgaaagtaa aaatgcatat gagttgcact aaaaaagcga ctcacattgt tccgttataa
    17341 tgcctgaagt agatcacaga atatatcttc agggatcgca tatctattaa gttactcact
    17401 cttttctatt tatgacatgc gcgtgtttgt ataaatgtaa atgtgagtcc ttgttccact
    17461 ctcgtgcagc atcgctggtc atacgcgaac acgtacaaac agcaatggtg aggcggcatc
    17521 aagagcaggg atccgtcatt tatgcgagca ttttacccca aaaacctttt atttataagg
    17581 gtcattgcga attatctgat gcaaagttat gttaggcaaa gtaatcttct attattaata
    17641 agcacatcaa aacctttttg aatattaaat aataattaat tagcatcatc ctcattcatt
    17701 aattccgctt aacaatagtt tcacaccttg cccccattgc gacacctcgg aaatcatcac
    17761 gctgttaaac tctccgtaat gcggaaatat catcatcgcg tttcattagt gaattcttaa
    17821 atgaggcatt ttcacacaat tatcttacag ataaaaaaac cagacttgca attaagaatc
    17881 agaacaattc actatataac attgcatgca aagcatatac gcctcattat tttgtcatta
    17941 ttaagttatt aacagcacaa tcgagccttc ccctctggca aaatcttatt ctgcagacct
    18001 tcaaaacacc gtcctggggg agtacattgt tctaagctga cttccacggc agggagtggc
    18061 gataacagca aaaaaggtca agattcatgt cccccattga aaaatccagc aaattagaga
    18121 atgtctgtta tgacatccgt ggtccggtgc tgaaagaagc aaaacgcctg gaagaagaag
    18181 gtaacaaggt actgaaactg aacatcggca acccagcccc attcggtttt gacgcgccag
    18241 atgaaatcct cgttgacgtg atacgcaacc tgcctaccgc tcaagggtat tgcgattcca
    18301 aagggcttta ctccgcgcgt aaagccatca tgcagcacta ccaggctcgt ggcatgcgtg
    18361 atgttaccgt ggaagatatt tacatcggca atggtgtatc ggagcttatc gttcaggcaa
    18421 tgcaggcatt gctgaacagc ggggacgaaa tgttggttcc tgcaccagat tacccactct
    18481 ggaccgcggc ggtttcgctt tccagcggta aagcggtgca ttatctttgc gatgaatcct
    18541 ctgactggtt cccggacctc gatgatattc gcgctaaaat tacgcctcgt acgcgtggga
    18601 tcgttattat caacccaaat aacccaaccg gcgcggtata ttccaaagag cttttaatgg
    18661 agattgtgga gattgcacgt cagcataatc tcattatctt cgccgatgaa atttatgaca
    18721 aaattctcta cgacgatgct gagcatcact caattgcgcc actggcacct gacctgctga
    18781 ccattacctt taacggactg tcgaaaacgt accgcgttgc aggcttccgt caggggtgga
    18841 tggtgttgaa cgggccgaaa aaacacgcca aaggctacat cgaaggtctg gaaatgctgg
    18901 cttcaatgcg cctgtgtgct aacgttcctg cgcaacacgc cattcagacc gcgctgggtg
    18961 gttatcagag catcagtgaa tttattaccc ctggcggtcg tctttatgag cagcgtaacc
    19021 gcgcgtggga actgatcaac gatattccgg gcgtttcctg cgtgaaacct cgtggtgcgc
    19081 tgtatatgtt cccgaaaatc gacgccaaac gctttaacat tcacgacgat cagaaaatgg
    19141 tgctggattt cctgttgcag gaaaaagttc tgttggtgca agggacggca ttcaactggc
    19201 cgtggccgga tcacttccgc attgtcacgc taccgcgtgt cgatgatatc gagctgtctt
    19261 tgagcaagtt cgcgcgtttc ctttctggtt atcatcagct gtaatcttaa tttcactgcc
    19321 ggagattgca tccggcagcg ttatcccgcc acaatgacct gatgatgtca tcatacgtaa
    19381 ggtcactatg aaacagagcc atttttttgc ccatctctcc cgcctgaaac tcattaaccg
    19441 ctggccgcta atgcgcaacg tgcggacgga aaatgtgtcc gaacacagtt tgcaggtagc
    19501 gatggtcgcc catgcgctgg cagctatcaa aaatcgaaaa tttggcggta atgtcaacgc
    19561 cgaacgtatc gctttactgg cgatgtacca cgatgcctca gaagtgctca ccggcgatct
    19621 ccctactccg gtgaaatact tcaattcgca aatcgctcag gagtacaagg ctattgaaaa
    19681 aatcgctcag caaaaactgg tcaatatggt tccggaagag ctgcgggata tctttgcgcc
    19741 gttaattgac gagcatgcat atagcgatga agaaaaatcg ctggtgaaac aggcagatgc
    19801 actgtgtgca tatctgaaat gtctggaaga actcgcggcc ggaaataatg aattcttgct
    19861 ggcaaaaacg cgactggaag cgacgcttga agcgcgtcgc agccaggaga tggactactt
    19921 catggaagta tttgttccca gcttccatct ttcgctcgat gagattagcc aggattcacc
    19981 gctgtaagca gccggagtct gcgtcgcatc aggcaataag cgccggatgc gacatcaggc
    20041 tcttgtcaaa acggaaacag catcgggatc atcaccacac aaaccgccat cacgataatg
    20101 gtgaacggta cccccaactt cacaaagtca ctgaagctgt aatttcccgg acctaaaacc
    20161 agtgtgttaa caggtgaaga aaccggtgtc ataaaggcgg cggatgctgc catcgccacg
    20221 accatcgcga atggataagg cgacaccccc atcgttttgg cagcagccag cgcaatcgga
    20281 gccatcaaca ccgccgtcgc ggtattagag ataaatagcc caataaccgc cgacaagaca
    20341 aacaaacacc ccagcatcat atgtggcccg taaccaccgc caatgtccat cagccctttc
    20401 accgccagcg cgacacctcc cgttttctgt aatgccacag caaacggcat catcccaacg
    20461 atcaaaataa tgctcggcca gtgaatggat ttataggcgc tttcagcatc tatacagcgg
    20521 aatttcccca tcagcaggca ggcgatgata gcggcaacag gattaggaat ttcatctgtc
    20581 agcattaacg ccaccattag caccagacag aaaatggcat ggggtgcctg gctgtgcgcg
    20641 ggcgatgctt cactcacctc ttccggtaag ttcagcgcta cgaagtcgcg gccctgtttg
    20701 gccagcatac cgatcagttt ccagttaccc acaaccagga tgatatcgcc cagcagcaga
    20761 ggctcatccg ccagcgaacc ttccagcgcc acgccattgc gctttagccc caccacattc
    20821 agtccgtagc gggtacgaaa accaatttcg cgcaccgatt taccaatcag ttctgactca
    20881 ggaattaatg aaatctctgc catgcccaca tcaagggcct ggtcagaaaa atactcgccg
    20941 cgcagtacca tcggctccag caattgctca ctacaaaatt gccggagatc gacatcagcc
    21001 gcagacatat caataagcaa aacgtcacgc gcgcgaaatt cagaaacccc attaacgttc
    21061 acgataacgc gacgaaaacg ccgccagcgt tcaacaccga tgacgttagc gccataacgc
    21121 tcacgtaatt tgagatcatc cagccgttga ccaatcattg gcgatccggg gcgaatagcc
    21181 agacgtcgcg cacgcccggt cagtcgatat tcacggataa gatcgcgaaa ggttcgacgc
    21241 gtccagcctt cgcgctgcgg ggtctgggta tcccctttca gcatgaaacg catcactaac
    21301 atatacaaaa tacccagcac cagcacgacc aggccaatag gtgttacgct aaagaaactg
    21361 aagccgtgat agccttcacg cagcaattca ctgttgacta ccaggttcgg cggcgtcgcc
    21421 accagcgtca tcatgccgct tatcagcccg gcaaaactca acggcatcat cagacgtgac
    21481 ggcgaagttt gcatacgcat ggcaacgctt aacaccacgg gaataaagat agcgacaacg
    21541 ccggttgaac tcataaacgc gccaagcccc gcgacggtca gcatcaacaa aaccaacatt
    21601 ttgatttcac tgttgcccgc aactttgacc agccatgttc ccattacggt ggcaacaccg
    21661 gtacggacca aaccatcgcc aataataaac aaggcggcaa tcaggacaac gttaggatca
    21721 gaaaagccgg aaaatacttc ggggactgtc agcgttccgc ttaatgcaaa cgcgacaata
    21781 acaaacaaag cgaccgcatc catacgcact ctgcccgtcg caaacaagac aatggcaacg
    21841 gccagtaatg aaagaaccca aatcaattca ccgttcacaa cttatccttg ttaattgagg
    21901 gagatgtctt gattctgcca taaaaaagcc ccgacgagac ggggctaaat catgatcagg
    21961 tgtttcactg aataataaca tcgccatttg gctgtttggt cacagtaatt tgctccagac
    22021 tgtggaggac caaatcgacc tcattcaggc gcggggtatc tgccggagcg ttaaccgcaa
    22081 tgacatgaca acccgccgcc aggccagaaa gcacgccagc ggagcatctt ccaccaccac
    22141 acactcctgc ggcgcaagcc ccagcagctg cgcgcctaac agatacgcat caggttctgg
    22201 ttttccgcgc ttcactcgct cagcggttac aaacacctct ggtgcgggaa gcccagctat
    22261 tttatggcgc gctcgcgcta ccggcatgga gccagaagtc acaatggccc acggaatacc
    22321 tgctttattc aaatgactga gtaaggcgat tgcccccgga agcgggtaat accttcggtt
    22381 tccgtggcct cgatgtgctc cagacgcgta aactcggcgg caatatcagc ctcggatttg
    22441 cccgccataa aatggcgcag agaggtgatc gcctgtttac cgtgaatgaa agccagcacc
    22501 tcttccggcg ctaacccatg acgtctggcc cagttgctcc acgcccgttc taccgcaggc
    22561 agggaatcca ccagcgttcc atcaagatca aacagaaaac ctttgcaccg cacgcgggcc
    22621 tcctcaggca ttgataattt gattaatttc gttggcgctc aaatggtact gacgcgggca
    22681 ggcatgccac acattaagca tgcgctgata tttttcccac attggcgtct gggcgttaaa
    22741 accgtgagtt ccggcgtcaa aatgggtata gcgcccttcc acattaacca taaagcggac
    22801 ataaccgagg taacgtgctt cagtggcggc gtcaaagccg aggaaggtga cacgacgttc
    22861 atcgatggat tgctggtcct gcaaattaga ccaggaaaca tgcaacgcat gatacatctc
    22921 cataatgtcg atgatagtgc ggcaggtttc ttctttcagc tcgccaaact cgcgatccaa
    22981 ttcacgcatc tgtaatccgt aacctcgctc aataattgtt tgcaggcgac ggtaacgttc
    23041 agcatttgcc ggatcgagca tagtcatcat cttgtactga ttagacaaaa taagacgttg
    23101 cgcgttggtc atttccattg ttgactcctg tatcactcta ctacggtgaa aaaaaagaag
    23161 gctgagtatg ccttctttta tatgcgtaat caggggtcaa ttacaaatca tcaaggaaag
    23221 ttttatccag ttgtttgaag gcgcgcttaa gcgtgtcagc taatgcctgg taatcaggct
    23281 tgccttcaac gggtgccaac acctgtccag actcctgcaa tttaccgcga acttcataaa
    23341 accagttaag gattgcaggg ggtaatggcg ttacagaacg cttgcccagc caccacaatc
    23401 cctgcatggg taaacttaag gcgaacagtg cagtggcaac tgccggccca agctgaccgc
    23461 ccagggcaat ctgccagcag agagtaaata cggcgatcgg cggcataaaa cggatcgcat
    23521 aacgcgtcat cttgataacg cgattttcga caaagaccgg ggcaaggcgt ttttccagtg
    23581 gccacgtctt tgagtaatgc tgtccgcggc gaaacaagct aaaaaaatta acagaacgat
    23641 tatccggcgt tgacatgctt cacctcaact tcacatataa agattcaaaa atttgtgcaa
    23701 attcacaact cagcgggaca acgttcaaaa cattttgtct tccataccca ctatcaggta
    23761 tcctttagca gcctgaaggc ctaagtagta catattcatt gagtcgtcaa attcatatac
    23821 attatgccat tggctgaaaa ttacgcaaaa tggcatagac tcaagatatt tcttccatca
    23881 tgcaaaaaaa atttgcagtg catgatgtta atcataaatg tcggtgtcat catgcgctac
    23941 gctctatggc tccctgacgt ttttttagcc acgtatcaat tataggtact tccatgtcga
    24001 gtaagttagt actggttctg aactgcggta gttcttcact gaaatttgcc atcatcgatg
    24061 cagtaaatgg tgaagagtac ctttctggtt tagccgaatg tttccacctg cctgaagcac
    24121 gtatcaaatg gaaaatggac ggcaataaac aggaagcggc tttaggtgca ggcgccgctc
    24181 acagcgaagc gctcaacttt atcgttaata ctattctggc acaaaaacca gaactgtctg
    24241 cgcagctgac tgctatcggt caccgtatcg tacacggcgg cgaaaagtat accagctccg
    24301 tagtgatcga tgagtctgtt attcagggta tcaaagatgc agcttctttt gcaccgctgc
    24361 acaacccggc tcacctgatc ggtatcgaag aagctctgaa atctttccca cagctgaaag
    24421 acaaaaacgt tgctgtattt gacaccgcgt tccaccagac tatgccggaa gagtcttacc
    24481 tctacgccct gccttacaac ctgtacaaag agcacggcat ccgtcgttac ggcgcgcacg
    24541 gcaccagcca cttctatgta acccaggaag cggcaaaaat gctgaacaaa ccggtagaag
    24601 aactgaacat catcacctgc cacctgggca acggtggttc cgtttctgct atccgcaacg
    24661 gtaaatgcgt tgacacctct atgggcctga ccccgctgga aggtctggtc atgggtaccc
    24721 gttctggtga tatcgatccg gcgatcatct tccacctgca cgacaccctg ggcatgagcg
    24781 ttgacgcaat caacaaactg ctgaccaaag agtctggcct gctgggtctg accgaagtga
    24841 ccagcgactg ccgctatgtt gaagacaact acgcgacgaa agaagacgcg aagcgcgcaa
    24901 tggacgttta ctgccaccgc ctggcgaaat acatcggtgc ctacactgcg ctgatggatg
    24961 gtcgtctgga cgctgttgta ttcactggtg gtatcggtga aaatgccgcg atggttcgtg
    25021 aactgtctct gggcaaactg ggcgtgctgg gctttgaagt tgatcatgaa cgcaacctgg
    25081 ctgcacgttt cggcaaatct ggtttcatca acaaagaagg tacccgtcct gcggtggtta
    25141 tcccaaccaa cgaagaactg gttatcgcgc aagacgcgag ccgcctgact gcctgatttc
    25201 acaccgccag ctcagctggc ggtgctgttt tgtaacccgc caaatcggcg gtaacgaaag
    25261 aggataaacc gtgtcccgta ttattatgct gatccctacc ggaaccagcg tcggtctgac
    25321 cagcgtcagc cttggcgtga tccgtgcaat ggaacgcaaa ggcgttcgtc tgagcgtttt
    25381 caaacctatc gctcagccgc gtaccggtgg cgatgcgccc gatcagacta cgactatcgt
    25441 gcgtgcgaac tcttccacca cgacggccgc tgaaccgctg aaaatgagct acgttgaagg
    25501 tctgctttcc agcaatcaga aagatgtgct gatggaagag atcgtcgcaa actaccacgc
    25561 taacaccaaa gacgctgaag tcgttctggt tgaaggtctg gtcccgacac gtaagcacca
    25621 gtttgcccag tctctgaact acgaaatcgc taaaacgctg aatgcggaaa tcgtcttcgt
    25681 tatgtctcag ggcactgaca ccccggaaca gctgaaagag cgtatcgaac tgacccgcaa
    25741 cagcttcggc ggtgccaaaa acaccaacat caccggcgtt atcgttaaca aactgaacgc
    25801 accggttgat gaacagggtc gtactcgccc ggatctgtcc gagattttcg acgactcttc
    25861 caaagctaaa gtaaacaatg ttgatccggc gaagctgcaa gaatccagcc ctctgccggt
    25921 tctcggcgct gtgccgtgga gctttgacct gatcgcgact cgtgcgatcg atatggctcg
    25981 ccacctgaat gcgaccatca tcaacgaagg cgacatcaat actcgccgcg ttaaatccgt
    26041 cactttctgc gcacgcagca ttccgcacat gctggagcac ttccgtgccg gttctctgct
    26101 ggtgacttcc gcagaccgtc ctgacgtgct ggtggccgct tgcctggcag ccatgaacgg
    26161 cgtagaaatc ggtgccctgc tgctgactgg cggctacgaa atggacgcgc gcatttctaa
    26221 actgtgcgaa cgtgctttcg ctaccggcct gccggtattt atggtgaaca ccaacacctg
    26281 gcagacctct ctgagcctgc agagcttcaa cctggaagtt ccggttgacg atcacgaacg
    26341 tatcgagaaa gttcaggaat acgttgctaa ctacatcaac gctgactgga tcgaatctct
    26401 gactgccact tctgagcgca gccgtcgtct gtctccgcct gcgttccgtt atcagctgac
    26461 tgaacttgcg cgcaaagcgg gcaaacgtat cgtactgccg gaaggtgacg aaccgcgtac
    26521 cgttaaagca gccgctatct gtgctgaacg tggtatcgca acttgcgtac tgctgggtaa
    26581 tccggcagag atcaaccgtg ttgcagcgtc tcagggtgta gaactgggtg cagggattga
    26641 aatcgttgat ccagaagtgg ttcgcgaaag ctatgttggt cgtctggtcg aactgcgtaa
    26701 gaacaaaggc atgaccgaaa ccgttgcccg cgaacagctg gaagacaacg tggtgctcgg
    26761 tacgctgatg ctggaacagg atgaagttga tggtctggtt tccggtgctg ttcacactac
    26821 cgcaaacacc atccgtccgc cgctgcagct gatcaaaact gcaccgggca gctccctggt
    26881 atcttccgtg ttcttcatgc tgctgccgga acaggtttac gtttacggtg actgtgcgat
    26941 caacccggat ccgaccgctg aacagctggc agaaatcgcg attcagtccg ctgattccgc
    27001 tgcggccttc ggtatcgaac cgcgcgttgc tatgctctcc tactccaccg gtacttctgg
    27061 tgcaggtagc gacgtagaaa aagttcgcga agcaactcgt ctggcgcagg aaaaacgtcc
    27121 tgacctgatg atcgacggtc cgctgcagta cgacgctgcg gtaatggctg acgttgcgaa
    27181 atccaaagcg ccgaactctc cggttgcagg tcgcgctacc gtgtttatct tcccggatct
    27241 gaacaccggt aacaccacct acaaagcggt acagcgttct gccgacctga tctccatcgg
    27301 gccgatgctg cagggtatgc gcaagccggt taacgacctg tcccgtggcg cactggttga
    27361 cgatatcgtc tacaccatcg cgctgactgc gattcagtct gcacagcagc agtaacctcg
    27421 tcatcatccg cagctttgcg ctgcggatat ctgaaccgga aataatcact atttccggtt
    27481 ttttattctc ttaatctgca ttaatccttt ctgattatct tgcttaactg cgctgcatca
    27541 atgaattgcg ccatttcact ttacatactt accactttgt tttgtgcaag ggaatatttg
    27601 cgctatgtcc gcaatcactg aatccaaacc aacaagaaga tgggcaatgc ccgatacgtt
    27661 ggtgattatc ttttttgttg ctattttaac cagccttgcc acctgggtag ttccggtggg
    27721 gatgtttgac agtcaggaag tgcagtatca ggttgatggt caaacaaaaa cacgcaaagt
    27781 cgtagatcca cactcatttc gcattctgac taacgaagca ggcgaacctg agtatcaccg
    27841 cgtacagctg ttcacgacgg gcgatgaacg cccgggcctg atgaacttcc cgtttgaagg
    27901 attaacctca ggatcgaaat acgggacagc tgttggcatc atcatgttta tgctggtgat
    27961 tggcggcgcg tttggcattg tgatgcgtac aggaaccatt gataacggta tcctggcgct
    28021 tattcgccat acccgcggga atgaaattct ctttattcct gcgctgttta ttctgttttc
    28081 acttggcggc gcggtatttg gtatgggaga agaggccgtc gcctttgcca ttatcatcgc
    28141 accgctaatg gtccggctgg gctatgacag tattaccacc gtcctggtga cctatattgc
    28201 cacgcaaatc ggttttgcca gttcgtggat gaacccgttt tgtgtggtcg ttgctcaggg
    28261 gattgccggc gttccggtgc tttctggctc cgggttgcgc atcgtggtgt gggttatcgc
    28321 cactatgatt ggcctgatct ttaccatggt gtacgcctca cgagtgaaaa agaatcctct
    28381 tctgtcacgc gtgcatgagt ccgaccgctt ctttcgtgaa aagcaggcgg atgttgaaca
    28441 acgtccgttt acctttggtg actggctggt attgattgtc ctgaccgccg taatggtctg
    28501 ggtgatttgg ggcgtgatcg ttaatgcctg gtttattcca gaaattgcca gccagttctt
    28561 caccatgggt ctggtgattg gcatcatcgg cgttgttttc cgccttaacg gcatgacggt
    28621 taataccatg gcttcatcct ttaccgaagg gacgcgaatg atgatcgccc ctgccctgct
    28681 ggtgggtttc gccaaaggga ttttgctgct ggtcggtaat ggtgaagcgg gtgatgccag
    28741 cgtgttaaat accatcctca acagcattgc caatgccatt agcggtctgg acaacgcggt
    28801 cgcggcctgg tttatgttgc tcttccaggc ggtatttaat ttcttcgtga cgtccggttc
    28861 tggtcaggcg gcgttaacca tgccgttact ggcaccgctt ggcgatctgg tcggtgttaa
    28921 ccgtcaggtt accgtgctgg cattccagtt tggtgatggc ttcagtcaca tcatttaccc
    28981 aacctcagct tcgttaatgg caactctcgg tgtgtgcagg gtggacttcc gtaactggct
    29041 gaaggtgggc gcgaccctac ttggactgct gtttattatg tccagcgtcg tagtgatcgg
    29101 cgctcagttg atgggctacc actaaaaatg ttaagagccg caattgcggc tcttttttca
    29161 ttctgccgtt tcagtctcta cggcttcatt tttggcattg cgcttcatcc acaacgccag
    29221 cgctttcagc gagtctggag tgaactcatc gcagcgtgcg gtgatttctt ccggcgtcag
    29281 ccagcaaact tcactgactt catcttcctg tagggcgaag ggaccgtgag agacgcagct
    29341 gaacaacgcg ccccagacac ggcaattttt atcttcgaaa tagaactgcc cgtgctcggc
    29401 aaaggggaca ccggcaatgc ccaactcttc ttccgcttcg cgacgcgcgg attccagcag
    29461 ttgctcatcg gcctgaacca caccgcctgc ggtcgcatct aacatgccgg gtaaaaagtc
    29521 ttttgtctcg gtacgacgct ggaccagaat tttgcccatg ccatcatgca cgacgatgta
    29581 agttgcacga tgacgcagac actgtgcccg catttgttcc cggctggctt gtgcaatgac
    29641 ttcgttctct tcattgacaa tatccaccca ttcagtactt gccaaacgac gctgttccat
    29701 tatcaggaaa ccttcttttt ctggcgctct tacggcgcat tttgagttgt gggtaaatta
    29761 cggattaatc gcgacctgcg caatgatact ttgatcattg agtgcgatta cactaagtac
    29821 gtcattatcc agcatgccat aactcgccgg attaccgcct ttcggaatac tcaccgagcc
    29881 ggggttgaag tggaaaatct ccccccgctg ttctgccact ggcagatggg tatgaccgta
    29941 caccagcaca tcgttctggt ttaaagcagg tagattttcc gggccaaaaa gatgaccatg
    30001 cgtcaaaaac agacgttgtt tttccagtaa tacctgttgc cacggcgcgg ttatcgggaa
    30061 atgcagcagc atttgatcca cttcgctgtc gcagttgccg cgcacagcga taaccttatg
    30121 tgccacttca ttaagccgtt cagcgacttt ggctggcgcg taaccctccg gtaaagcatt
    30181 acgcgggccg tgattcaaca cgtcgccaag gatcaccagc cactgggcac cgctttgggc
    30241 aaacaactcc agaacacgtt ccgtcgccgg taacgaccca tgaatgtccg atgcaaacat
    30301 cagtttcatc actcactcct cgtcgaaaaa acgtaagcta tgatactgga ttctgtcgtt
    30361 gctatcagcc agattgcttc gccgaaagtg caataaaacg ctggacagac gctcgctgcc
    30421 actggaatgt cgcataatcc accaggcgtt ctggcacctc atcgccatgt agtaccaggc
    30481 ggttaatcat tagcgccaga tcagtatcag caatgcacca ttcaccaaat aaattcggct
    30541 gacccagtgc taacaaatgt tctgccatcg cgaacagttt ctctgcactg gcttttccct
    30601 cggccgttag tggcgctttt ttcgcccccg caaagacaac atccgtcgga cgctcttcgc
    30661 ggatgggcat cagatcgctg cgcagccagg cctgaatccg tcgtgcacgc gcacgatttt
    30721 ctaaatcaag cggataaata cgttcccagg tcggtggcgc aaatcgatct tccagatact
    30781 ccgcaatggc agaagattca ctcaactcaa aatcatcgat ttgtaataac ggcacacggc
    30841 gtgtctgacc gtaaccttgc cacgtcggct tcaaatgttc accgctgtcg aggtcgatgg
    30901 tcttgatatg aaacgacagg cctttttcct gcaacgccac ccaggcggat aacacataag
    30961 gggagaaaaa gtgggcatct gaccaaagcg tgatagcggg tttactcatt atgtcctcgg
    31021 caataaaggg ggctggctat caaactatag agcctttttg tcactgtcac gtgacgaata
    31081 ctcacgtaag tcgccgcgct ttctatactt gtggtgttca gatgattaaa caaccggagc
    31141 tgcaatgatc gatctctatt tcgccccgac acccaatggt cacaaaatta cgctgtttct
    31201 cgaagaagca gagcttgatt atcgcttgat taaggtagac ctggggaaag gcggtcagtt
    31261 tcgtccggaa tttttgcgca tttcgcccaa caacaaaatt ccggcaattg ttgatcattc
    31321 tcctgccgat ggcggcgaac cgctaagcct ctttgagtct ggtgccattt tgttgtatct
    31381 gactgagaaa acaggactct ttttgagtca tgaaacgcgt gagcgcgccg ccacattaca
    31441 gtggttattc tggcaggtag gcggactggg gccgatgctt gggcaaaatc atcattttaa
    31501 tcacgcagcc ccccaaacca ttccttacgc tattgaacgt tatcaggttg aaactcagcg
    31561 tctttaccat gtactaaaca agcggctgga aaactcgccc tggctgggag gcgagaacta
    31621 cagcattgcg gatattgcct gctggccgtg ggttaatgcc tggactcgcc agcgaattga
    31681 cctcgcaatg tatccggcag tcaagaactg gcatgagcgg atccgttcgc gccctgccac
    31741 cgggcaggca ctgctaaaag cacaactcgg tgatgagcgt tcggatagtt aacagaaaca
    31801 ggttctcgtg tattatttca tcctaagtaa aacaacggag aacctgcaat ggcacaacct
    31861 gccgctatta ttcgtataaa gaaccttcgt ttacgtacgt ttatcggaat taaggaagaa
    31921 gaaattaaca accgtcagga tattgttatc aatgtgacga tccactaccc cgccgataaa
    31981 gcgcgcacta gcgaagatat caacgatgcg ctgaattatc gcaccgtaac gaaaaacatt
    32041 attcagcatg tagagaataa ccgtttctct ttgttggaaa aattaactca ggatgtgctc
    32101 gatatcgcac gtgaacatca ctgggtgacg tatgctgaag tggagatcga taaactgcac
    32161 gcgctgcgct acgccgattc ggtatccatg accttaagct ggcagcgtta accgccatat
    32221 cgggaggctg catgaacata gtgatcaccg gagggacggg attaattggt cgccatttga
    32281 ttccgcgttt gctggagctg ggccatcaaa tcacggtagt gacgcgtaac ccacagaaag
    32341 ccagttccgt tctcggccct cgggtgacgc tatggcaagg gctagccgat caaagcaacc
    32401 tcaacggcgt tgatgcggta atcaacctgg ccggagaacc gattgctgat aaacgctgga
    32461 ctcacgagca aaaagagcgt ctctgccaaa gccgctggaa tatcacgcaa aaactggtcg
    32521 atttgattaa tgccagcgac acgccgccgt cggtattcat ttccggctcg gcaacgggct
    32581 attatggcga cttaggtgaa gtggtggtta ccgaagagga accgccgcat aacgaattta
    32641 ctcataaact ctgcgcccgc tgggaagaga ttgcctgccg ggcgcaaagt gacaaaacgc
    32701 gagtgtgcct gctgcgaacc ggcgtggtac tggcaccgga cggcggtatt ctcggtaaaa
    32761 tgctgccacc gtttcgtctt ggcctgggcg ggccgattgg ttccggtcgg cagtatctgg
    32821 cctggattca tattgatgat atggtcaacg gcattctctg gctgctggat aacgacctgc
    32881 gcgggccatt taatatggtt tcgccctacc cggtacgcaa tgaacaattt gcccatgcgc
    32941 tcggtcaagc gctgcatcgc ccggccattt tgcgcgtccc cgcaaccgcc attcggctgt
    33001 taatgggcga atcttcaata ctggtattag gtggacaacg cgcgctgcct aaacggctgg
    33061 aagaagcggg ttttgcgttt cgctggtacg atttagaaga ggcgctggcg gatgtcgttc
    33121 gctgatgtgg tttacagcaa acatccgcca gttaactccc ggtgttacag gattagtggc
    33181 tttgcgcgat aagatcgtct ggtgaaagtc gggtcaccat cataactaac tctctgtcta
    33241 aacctctatc cagcatctcc tgagcaatac gcagggcttc ttcgtgtttg ccctgcattg
    33301 cgccttcttc acgtaatctg tcagcaatgg tcatcagttt ctccttttct tgtggtgcgc
    33361 gttctgttat ctcaccaata aatgcacgga aacgctgggc atcccctgtt tgtaatacgt
    33421 aattaaacag ggcttttagc tgtctgtcat tagtgttccc tgtaactagc agcgaaacaa
    33481 tttggtcgac taatcccaac agatcgcgct gacgaatatg tttctgaatt aactccaaca
    33541 gcgccatttt gcggtgttgc ataatctcgt catccggcac cacggtaata tccaccaacg
    33601 gaaacgctga tgaatatatt ttgcgggcta tagcaggctc ggcaaattca tcaagccagc
    33661 agagtgaata aggataagga cttctgcaac catgataaaa cagcatcggg atcaccaatg
    33721 gaagctcttt atagcccgca tcaagatggt tttgcattgc cgcaatggaa taacgcatca
    33781 tgcgaaaagc cattaattct tccagcttac tttggtgctc tatcactaca taaatataac
    33841 ccactccctc ctgcgttttc acagaccaca agaggtcgga ataatattgc cgcaggtctt
    33901 catcaataaa actgtttgat tctagtttaa gcgtcgttaa atcacacagt ttgcgcagcg
    33961 gcgcgggaag atgaatatca ataaaatccc gcgcggtgt
//
LOCUS       DAJTZL010000026        33995 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.026, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000026 DAJTZL010000000
VERSION     DAJTZL010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33995
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.026"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(1..2601)
                     /gene="acrF"
                     /locus_tag="OO544_001083"
                     /pseudo
     CDS             complement(1..2601)
                     /gene="acrF"
                     /locus_tag="OO544_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
     gene            complement(2613..3770)
                     /gene="acrE"
                     /locus_tag="OO544_001084"
     CDS             complement(2613..3770)
                     /gene="acrE"
                     /locus_tag="OO544_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HCR6766734.1"
                     /translation="MTKHARFFLLPSFILISAALIVGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     NYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     ATVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDECTGSITLR
                     AVFPNPQHTLLPGMFVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNDKSQVEAR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
     gene            4169..4831
                     /gene="acrS"
                     /locus_tag="OO544_001085"
     CDS             4169..4831
                     /gene="acrS"
                     /locus_tag="OO544_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HCR6766735.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTFNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQEHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDGMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIIDGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHQTNELS
                     VM"
     gene            complement(4834..5013)
                     /locus_tag="OO544_001086"
     CDS             complement(4834..5013)
                     /locus_tag="OO544_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HCR6766736.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLTTETDKCRNMNSVYP
                     LKLVNCDELNFQDRM"
     gene            complement(5097..5981)
                     /gene="yhdJ"
                     /locus_tag="OO544_001087"
     CDS             complement(5097..5981)
                     /gene="yhdJ"
                     /locus_tag="OO544_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HCR6766737.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKLPTESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIVECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            complement(6067..6363)
                     /gene="fis"
                     /locus_tag="OO544_001088"
     CDS             complement(6067..6363)
                     /gene="fis"
                     /locus_tag="OO544_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HCR6766738.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(6389..7354)
                     /gene="dusB"
                     /locus_tag="OO544_001089"
     CDS             complement(6389..7354)
                     /gene="dusB"
                     /locus_tag="OO544_001089"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HCR6766739.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(7683..8564)
                     /gene="prmA"
                     /locus_tag="OO544_001090"
     CDS             complement(7683..8564)
                     /gene="prmA"
                     /locus_tag="OO544_001090"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HCR6766740.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     IVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYVDSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(8576..10027)
                     /gene="panF"
                     /locus_tag="OO544_001091"
     CDS             complement(8576..10027)
                     /gene="panF"
                     /locus_tag="OO544_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HCR6766741.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(10017..10259)
                     /locus_tag="OO544_001092"
     CDS             complement(10017..10259)
                     /locus_tag="OO544_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HCR6766742.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVAAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(10368..11717)
                     /gene="accC"
                     /locus_tag="OO544_001093"
     CDS             complement(10368..11717)
                     /gene="accC"
                     /locus_tag="OO544_001093"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HCR6766743.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     RGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(11728..12198)
                     /gene="accB"
                     /locus_tag="OO544_001094"
                     /gene_synonym="fabE"
     CDS             complement(11728..12198)
                     /gene="accB"
                     /locus_tag="OO544_001094"
                     /gene_synonym="fabE"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HCR6766744.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(13176..14150)
                     /locus_tag="OO544_001095"
     CDS             complement(13176..14150)
                     /locus_tag="OO544_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR6766745.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            14302..16242
                     /gene="csrD"
                     /locus_tag="OO544_001096"
     CDS             14302..16242
                     /gene="csrD"
                     /locus_tag="OO544_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HCR6766746.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSTLRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGSSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIIVMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIAGQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTHVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            16547..17590
                     /gene="mreB"
                     /locus_tag="OO544_001097"
     CDS             16547..17590
                     /gene="mreB"
                     /locus_tag="OO544_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HCR6766747.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            17656..18759
                     /gene="mreC"
                     /locus_tag="OO544_001098"
     CDS             17656..18759
                     /gene="mreC"
                     /locus_tag="OO544_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HCR6766748.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPTTGNAATA
                     PAAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            18759..19247
                     /gene="mreD"
                     /locus_tag="OO544_001099"
     CDS             18759..19247
                     /gene="mreD"
                     /locus_tag="OO544_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HCR6766749.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            19256..19849
                     /gene="yhdE"
                     /locus_tag="OO544_001100"
     CDS             19256..19849
                     /gene="yhdE"
                     /locus_tag="OO544_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HCR6766750.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            19839..21308
                     /gene="rng"
                     /locus_tag="OO544_001101"
     CDS             19839..21308
                     /gene="rng"
                     /locus_tag="OO544_001101"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HCR6766751.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            21376..25176
                     /gene="yhdP"
                     /locus_tag="OO544_001102"
     CDS             21376..25176
                     /gene="yhdP"
                     /locus_tag="OO544_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HCR6766752.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWHYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQYITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            25332..26777
                     /gene="tldD"
                     /locus_tag="OO544_001103"
     CDS             25332..26777
                     /gene="tldD"
                     /locus_tag="OO544_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HCR6766753.1"
                     /translation="MSLNLVSEQLLAANSLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(26905..27770)
                     /locus_tag="OO544_001104"
                     /pseudo
     CDS             complement(26905..27770)
                     /locus_tag="OO544_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
     gene            27953..28156
                     /locus_tag="OO544_001105"
     CDS             27953..28156
                     /locus_tag="OO544_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AaeX family protein"
                     /protein_id="HCR6766754.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            28164..29096
                     /gene="aaeA"
                     /locus_tag="OO544_001106"
     CDS             28164..29096
                     /gene="aaeA"
                     /locus_tag="OO544_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HCR6766755.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGGRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            29102..31069
                     /gene="aaeB"
                     /locus_tag="OO544_001107"
     CDS             29102..31069
                     /gene="aaeB"
                     /locus_tag="OO544_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HCR6766756.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVFAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            31161..31433
                     /locus_tag="OO544_001108"
     CDS             31161..31433
                     /locus_tag="OO544_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HCR6766757.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(31489..31752)
                     /gene="yhcN"
                     /locus_tag="OO544_001109"
     CDS             complement(31489..31752)
                     /gene="yhcN"
                     /locus_tag="OO544_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HCR6766758.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDTAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(32117..32587)
                     /gene="argR"
                     /locus_tag="OO544_001110"
     CDS             complement(32117..32587)
                     /gene="argR"
                     /locus_tag="OO544_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HCR6766759.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            33022..33960
                     /gene="mdh"
                     /locus_tag="OO544_001111"
     CDS             33022..33960
                     /gene="mdh"
                     /locus_tag="OO544_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HCR6766760.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
BASE COUNT         8254 a         8453 c         8900 g         8388 t
ORIGIN      
        1 tcctgatacg acatacctgt ccagtcataa ccaatgcccg caggtaattt tgacgcaagg
       61 ttttccatca atgccatggc atcgccggaa ctggttcctg gcgcggcttc cccctgaatc
      121 tccattgacg gcagaccgtt gtagcgttcc agtcgcggag agccatacac ccaatgtgaa
      181 gtggtaaagg cagagaatgg caccatttcg ccgttggcgc tgcggacata aagtttatcg
      241 acatcttctg gcagcatacg gaatttggca tccgcctgaa catacacctt tttcacgcga
      301 ccacggtcga tgaagtcgtt aacgtaagtc ccacccagcg ccgttgaaat ggtctgattg
      361 atgtcagaaa gtgaaacacc taatgcctgc gccttttcct ggtcaacttc cagtttaaac
      421 tgcgcggtgt cttccagacc attaggacgc acgctgacta agctggcagg atgttgcgcc
      481 gccatgccaa gcaactggtt acgggcctgg gtcagggcat cgtgacccag cccagcctga
      541 tcaattaact caaagtcgaa acccgttgcc gtgcccagtt caacaatggc tggcatattg
      601 aatggaatga caaaaccgtc gcggatcttg cccaattcca ttttggcacg atggattacc
      661 gcttccgcac tgttttcgtc accactacgc tcttcccacg gtttcagact gacgaaagcc
      721 ataccggcgt tttgtgcctg gccgctgaag ctaaagccgt taaccgtaaa gacactttct
      781 acgttcgctt tctcgttatt cagatagtaa tccgtcactt gatccaacac tttttgcgtc
      841 cgctcttgcg tcgcgccagc gggtaactga atcatggtca gaaagacacc ctgatcctct
      901 tcaggtaaga aggaagaagg aagacggaag acgtaaaaac aacaccacca ttcctgcaac
      961 aatcagcgca tagatcagta aatatcgtcc tgtggaaccg aggattttgc cgacgctgtt
     1021 ggtgtagtgg ttaacgctat gatcgaaggt ggtattaaac cagccgaaga aaccgccctt
     1081 attttcgtga tgctcagcag agacgggttt aagcagcgtt gcacataacg caggggtaag
     1141 aatcaatgcc accagaacag aaagcgccat tgccgaaacg atggtgatag agaactggcg
     1201 ataaattgcc ccagtagaac cgccgaagaa tgccatcgga ataaataccg ctgacagcac
     1261 catcgcgata cccaccagtg cgccctgaat ttgcgacatc gatttttccg tcgcttcttt
     1321 tggcgggagc ttatcctcca tcatcacgcg ctcgacgttc tccaccacca ctatcgcatc
     1381 atcgacgagc agcccgatgg caagcaccat cccgaacatc gttagtgtgt tgatggagta
     1441 accaaaagcg gcgaggatgg caaacgtccc taacaacacc acgggtaccg caatggtggg
     1501 gatcagcgtt gctcgcatat tctgcaagaa cagatacatc accaggaaca ccagcataat
     1561 ggcttcgaac agcgttttta ccacttcgtg aatagaaagc tggacgaatg gcgtggtgtc
     1621 ataagggtag agaaccttca ttccctgcgg gaagaatggc tgtaattccg ccagttttgc
     1681 cttaatggct ttcgcggtat tgagagcgtt cgcgccggtt gctagcttaa tccccaggcc
     1741 cgccgccggt tttccgttga tacgagcgat aacgttatag ttttcaccgc caagttcaac
     1801 tcgtgcgaca tctttcaggc gtaccaccga gccgtcactg tttacgcgca gggttacttt
     1861 gccgaattct tccggatttt ttaaccgcgt ctgagcaata atcgaggcgt tcaactgttg
     1921 ccctggtaac gctggcgttc cgcccaactg cccggcagcg atctgatcgt tctgtacctt
     1981 caactggtta atcacatcaa ccggtgtcag tttatatttg tttagcagat cggcgtccag
     2041 ccagatacgc atcgcatact gtgcgccgaa aagctgtacg tcaccgacgc cattcagacg
     2101 gctaagcgta tctttaacgt tagaggccac atagtccgag atatcgtcct gtgtggtgtc
     2161 tgggttatca gagacaaagc ccgccaccat caaatagctg ctactggact tttcaacact
     2221 gatcccctgc tgctgaacct cctgcggcag caacggcgtg gcgagctgga gtttgttctg
     2281 cacctgcact tgcgcgatat caggatcggt cccggactgg aaggtaaggg taattgtcac
     2341 gctaccggcg gaatcgctgg tggaggacat atacatcagg ttatcgatac cattcatatt
     2401 ctgttcgata acctgcgtca ccgtatcctg cacggtctgc gcatcagcac ctggatagtt
     2461 tgcctaaaca gaaaccgcag gcggtgcaat ggttggatac tgagcgacgg gcaattgtag
     2521 gatcgccagt gcgcccgcca tcatcagaat aatggccagc acccatgcaa atatcggtcg
     2581 tcgaataaaa aagtttgcca tgtcagatta ccttacttcg atgcagtatc tgcgggggta
     2641 tcggtagtgg ctttaacctg ctctcccgga cgcgcttttt gcaggccgct gacaatgact
     2701 tgatcgccag atttcagacc ttcgctaatc aaccatttat cgccaatcgc ctgactggca
     2761 acgacagggc gcgcttccac ctgactttta tcgttaacaa tcagcacggt tgcatcacca
     2821 cgcggtgtgc ggctaacgcc ttgttgcggg ataagaatgg cgtcaggttg gacgccttca
     2881 tcaatccgtg cacgcacaaa catacccggc aaaagcgtat gttgcgggtt agggaagaca
     2941 gcacgtaggg ttatggagcc ggtgcattca tcaacggtca catcagagaa ttgcagcgta
     3001 cctttcaggg gataggtttg accgttttcc atgaccaact ctacgttgct ggtggcgttt
     3061 tccttatgca aatttccttg ctctacggat tgcttcagcc tcataaaatc gttgctggat
     3121 tgggtcacat caacgtagat aggatcgagc tgctggacag tcgccagttc agtcgtttgc
     3181 ccattagtga caagagcgcc ttcggtcacg gtcgatttgc cgatacgtcc gctaattggc
     3241 gcagtcactt tggtataagc gagattgatg cgagcgcttt cgactgtggc ttttgcggca
     3301 atcacggcgg catcggcctg gcgagcatca gcaatggcct ggtcgtactc ctgctggctg
     3361 atgtatttcg tacccacgag cggaacgtaa cgttttaccg tcaaatgcgc gatggcggcg
     3421 gcggcttcac ttttcgccag ttcgcctttc gcgctgtcat aatttgcctg ataggtcgcg
     3481 ggatcgatct ggtacaggga ctggcctgct tgtacatcgc tgccttcagt gaaattacga
     3541 ttcagtacga tcccgctaac ctgtgggcga acttcggcta tacggtaagc attggtgcgg
     3601 cctggtaatt cagtcttaac ttctaacggg gccgttttca caatatgaac ggtaacctgc
     3661 ggttcaccga cgtgagcttt ctcttctccc ttatcgttac aaccgacgat taaagccgcg
     3721 gagatcagaa taaaggaggg caggaggaaa aacctggcat gtttcgtcat tactattcct
     3781 caaaaaaaca aaagcgcgtt atttacccag aaaggcaacg cgttaactcg cagaaagaaa
     3841 aatacagttt gctatcctac aaattatcat tcgtcgatgt aaggaatagt tatgaatact
     3901 ggcgtctcaa ggcacataaa cacaaaaaaa gattaatatt ctactgtttt attttgacgc
     3961 gggttgaaag aggcagaatt aaaacctcgt aaattgaaat atatattcat gtggcgaatg
     4021 tattttaggt aaataatata tattatttat ttgacgattt atagcaactc aatgttaccc
     4081 tctcgtataa atacacatta gatgatagat taaccttcgc tattttctca ccctgtgtcg
     4141 aatatattta tttcctgaat aattaatcat ggcaaaaaga accaaagccg aagctctgaa
     4201 gacccggcaa gaactgattg aaactgccat cgcccagttt gcgcaacatg gcgtaagcaa
     4261 gacgacgttc aacgacattg ccgatgccgc taacgttacg cgtggcgcta tctactggca
     4321 cttcgagaac aagacccaac tgtttaatga gatgtggttg caacagcctt cattgcggga
     4381 gttaatccag gaacacttga cggctggatt agagcatgac ccgtttcaac aattgcgtga
     4441 aaaattgatt gtcggcttgc aatatattgc caaaattccc cgccagcagg cgttgctgaa
     4501 aatcttatat cacaaatgtg aatttaatga tgggatgctg gccgagggag tgatacgcga
     4561 aaagatgggc tttaatccgc agactctccg cgaagtattg caggcgtgtc agcaacaagg
     4621 ttgtgtagca aataacctcg atttagatgt tgtgatgatt attattgatg gtgccttcag
     4681 cggaattgtt caaaactggt taatgaatat ggcgggttat gatctttata aacaagcacc
     4741 cgcgctggtc gataacgtat taagaatgtt catgccagat gaaaacataa cgaaattaat
     4801 tcatcaaaca aatgaattaa gtgtcatgta aattcacatt ctgtcctgaa aattcagttc
     4861 gtcacagtta accagtttta gtggataaac tgagttcata ttacggcatt tgtctgtttc
     4921 tgttgtcagc agttcaatcc actgcatcag taaagtatca aacagaaaaa cgaagacagc
     4981 gaaaacgacg agccaccaat acttgcgaat cattacctga gcctgtgcat gtttaaccaa
     5041 gtctggcagg aaatatacac gaaatgaata atctgaaaac caggcttaca cttcctttac
     5101 tttgcaatga gatcggggtc aacttcgctt aaccggcttc gttttgacag gttgccggtc
     5161 tttctttttt tcactttcgc cagttcttcc gcggagtaat gcgacgcgac atccagccgt
     5221 cgaagcccca ttttgatgta ctcgctgttg atctcaatac caatgaattt tcgtccgctg
     5281 gcgatggcta cggcaccggt agtaaagcta ccggcaaacg ggtcgagaac gatatcgcct
     5341 gggttggaag aggcgagaat aatgcgtttc agtaaggctt ccggtttttg cgtcgggtgg
     5401 ttctcatatt catccatcaa ataacgcacg cgcggaaaat cccagacgtt acccggtact
     5461 ttttgatgat tgtatggctg tggaggattt ttgcgataat cgatcaacgc gcgctgcgat
     5521 ccggttttgg cttctaccag aatagcatca ccgttgaatg tgtagttctt tgcgtctttc
     5581 accatcatca ggatgggttc gtacatggag ccgtagtgtt ttttcgcctg tactccagaa
     5641 ctgtcatatg accagacgat gcgacttttg atggtaaaaa gcttgcggca ctggagatcg
     5701 ataaagggca tgttttccgt actgttcata atgtacatgc tgccctgctt tttcagaacg
     5761 cggtggcact ctacaatcac ttcaaacagc cagtcgataa acagatcttc tttccaggct
     5821 tcgatcagac catcaaaatt tttaccgatg ttatacggtg ggtcggcaaa gatcagatcg
     5881 acgctttcag taggtagctt tttaagttcg gcaagggcat caccgtgaat aatggtctta
     5941 gcttcattac caaaccgggt cggttcacat cctgttctca tggtcactcc ctttgtgaca
     6001 cctataaaaa aggcgcttcc ccatgccgag tagcgccttt ttaatcaagc atttagctaa
     6061 cctgaattag ttcatgccgt attttttcaa ttttttacgc agcgtaccac ggttgatgcc
     6121 catcatcagc gcagcacggg tctggttacc acgggtgtat tgcatcacca tgtccaacag
     6181 gggctgttct acttcagcca gtaccagctc atagaggtca ttcacatcct gaccattcag
     6241 ttgagcaaaa tagttcttca gtgcctgttt aaccgagtca cgcaggggtt tttgggttac
     6301 ctgatcctga gagttaacgg tagaaacggt cagtacgtca gaatttacgc gttgttcgaa
     6361 catagttctg tcagctcttt atttctgttt acgcaaaatt ttcgaagtat gcctccaacg
     6421 cctccagctg ttcgctggca tcctcaatgg cgttgaatgt gcgccgaaac tggtcatttg
     6481 gagcgtgttc ctggagatac caggaaacgt gtttacgtgc aattcggtac ccttttgccg
     6541 gaccataaaa gtcatgcagt tcccgaacgt gcgcgcaaag caagcgctta acctctgcca
     6601 aaggcagcgg gggcagcaac tccccagtgt ccagataatg ctggatttcc cgaaagatcc
     6661 agggtcttcc ctgagctgcg cggcctatca tcagggcatc cgcccctgta tagtcgagca
     6721 cagctctggc tttaagcggg tcagtaatgt cgccattcgc gataaccgga atggaaactt
     6781 tctgcttaac tgcccgaata ctgtcgtact cagcttctcc attgaacaaa caggcgcgtg
     6841 tacggccatg aatggtcaga gcctgaatgc cacagtcttc agccagttgg gcaatctctt
     6901 cgcagttacg gtgttccggt gcccagccgg tgcgaatctt cagggtaaca ggaacgtcca
     6961 ctgcattgac gacctcggta aggatcgatt taacgacatc cgggtactgc aagagggctg
     7021 aacctgcgag cttgcgattc acttttttag ccgggcaacc catattgata tcaataatct
     7081 gggcaccgct ttccacgtta atacgtgctg catctgccat ttctttcgga tcgctaccag
     7141 caatttgcac ggtgcgaata ccgggttcat caatgtgcac catccgtaaa cgagatttgt
     7201 cgctttccca aacctgtggg ttagaagaca tcatctcgga tactgtcaat ccggctccca
     7261 tctcgtagca caacgtccga aaaggtctgt ctgtaatgcc agccatgggc gctgcgatca
     7321 ggcgatttct gagctgatat tgtccgatgc gcatgagtta agaaatgacc atactgtgac
     7381 tgcaaggcgg cgtatattac gcattttttg cacgagatga aaggccaaac tttgaccaat
     7441 cctctgagat ggatcaaaga attgcattta aaatgagcgt ggtgagataa ttactcataa
     7501 aaatcatcat attagaaaat agtgactaaa atttacactc aaagaaattt gagtaagttc
     7561 tcaatttttc tttatgaatg aaaattttgg cacgcaaatt tgcgtaaata accggcaaat
     7621 ttacgtgcct tttgtgagct tgctcgcact tcgccccgcg tcaccctacg gcgatgcgaa
     7681 ggttaattct tacgaccggt aatgcggcac cactcttctt tttccacgac cgggtccagt
     7741 gcgaagctgt cgacataagc ttcacaaacg ctctctgcct ggcttgccag aataccggaa
     7801 aggcccagca aaccgcctga aaccggcagg acgctgatta acggtgccag ttcacgtaat
     7861 gggcctgcaa ggatgttagc gaccaccacg tcggctttca tttcttctgg ctgatctttc
     7921 ggtaagtaga gttccagacg gtcagaaacg ccattacgtt cggcgttgtc gcggctagcc
     7981 tgaatcgcct gcggatcgat atcaatacca atggcttttg ctgcacccag tttcagcgcc
     8041 gcgatagcca gaatgccgga accacagcca aagtcgatga ctattttacc ggttaaatcg
     8101 aggctgtcga gccactgcag gcacagagag gtagttggat gggtacccgt accaaacgcc
     8161 agccctggat ctaacatcac gttgacggcg ttttcgtccg gcacatcacg ccagctaggg
     8221 cagatccaca gtcgttcacc aaagcgcatc gggtggaaat tatccatcca ttcgcgctcc
     8281 cagtctttat cttccagttg ttcgatttta tgcgcgaagc ctgcgccgag cagcggatgg
     8341 ttttccagaa tcgccaccac gtcgttcata tcggtttcag cgtcgaacag accaatcaca
     8401 tcggtgtcgc cccacaggcg cgtttcgccc ggcagcggct caaatactgg cgtatcgtgg
     8461 gtatcctgaa aagtgataga aacggcaccc gcttccatca gcgcatcgct aagatcttca
     8521 gcgttcgcgc cggtggtgtt cagtttcagt tggatccaag gcatggcaaa actctttatt
     8581 tatcagtagt caaaacggta gcttgcggga cggatgtacc gaaacggttt ccgaccagga
     8641 aagccagcaa acttagtagt aacgagggca cgatagggtg gaagcccagg tactgaatat
     8701 tcagcgtcgc gagtacggca tacagcacgc cgccaacgat catcgcactt agcgcgcctt
     8761 tggcgttggc gcgttcccag taaagaccca gcaccagcgg ccacaggaaa acggcttcca
     8821 gcccaccgaa ggccagcaaa ttcagccaga tgatcatttc tggcggcttc caggcggcaa
     8881 gcagcagcaa cgcgccgaga actaacgtaa ttaccgccga catccgcttc agacgcgtct
     8941 cgttttgcat ttgatccgga cggatattca gatagagatc tttaatgatc gtagcggaac
     9001 tttgtagcaa ttgggcgtta attgtcgaca tgatcgcagc cataggtgca gccagaaaga
     9061 tcccggcagc aaacggtggc agcactttta ccattaacgt cgggatcacc agatccggta
     9121 cggtgagatc ggggatcact gcacgaccta acgctccggc taggtgcata ccgaacatca
     9181 ggattgcgac tacaatcgta ccgatgatga tcccccggtg taccgctttg ctgtctttat
     9241 aagagataca gcgcaccgca gtatgcggca ggccaatcac gccaaagcac accagtaccc
     9301 agaacgacgt cataaaggca ggcgacagaa tatcgtcagc gccttgtggc gtaaccagtt
     9361 gcggatcgat ggtttgcaag gtctgtactg cgttgcttaa gccgccagcg gcatgtacca
     9421 cgccaataag cagcacaacg gtgccaatca gcatcacaag cccttgcatg gtgtcgttca
     9481 gcacgctggc gcgaaagccg ccaaaggcgg tatacaacgc aatgctgata ccgaaaatca
     9541 gcagcccggt ttcataagga atacccgccg cggtttccag caggcgcgca ccgccgataa
     9601 actgcacggt cattgcccca acgaacgcaa ccagcaaact caaactcgcc agccacacca
     9661 gaagacgact ctggtagcgg gcaaacagca tgtcgttcag cgtcactgca ttgtagcggc
     9721 gcgcaagaat cgcaaacttc ttgccgagaa taccgagtga aagccagact gcaggaagct
     9781 gaatcatcgc cagcaatacc cagcccagcc cgtatttata agcagctcct ggcccgccga
     9841 taaacgaact ggcactgata taggtcgcgg tgagcgtcat cgccagcaca ataccgccca
     9901 tagagcggct gccgaggaaa tactcattaa ggaaggtgcc ggtgctccgt ttacgcatcg
     9961 cataaaccga gataccgaac accaccacca gataggcgac cagcggtaga attacttcaa
    10021 gctgcatcgt catcctccag tgggatatcg cgatagataa atttcaccat cgcccagcac
    10081 agtccaataa acagtagcgg cgtcaggatg caggccatct caaaccagcg cggaaagccg
    10141 gtaaaaccgg gggcaacgcc aggtaagtaa gcggctacta accaaactgc cagatacaaa
    10201 agggtcagcc ccagcgccca gcgcgcctct ttatgggcct gaacaaaacg agtgtccatt
    10261 ttttgtccct gatgggtgaa gaaagcgagg attgtacctt atgggggttg tcgatcccca
    10321 gtaataaaaa aggccggaaa atccggcctt ttggcgcttt tgcagtctta tttttcctga
    10381 agaccgagtt ttttctccag atagtggatg ttagtgccac catgctggaa gttctcgtca
    10441 ttcatgatgc ggatctgcag atcaacgttg gttttgatac cgtcgatgat cagctcctgc
    10501 agcgcattct tcatgcgggc aatcgccacg tcacggtttt caccgtagca aatcagctta
    10561 ccgatcattg agtcatagta cggcggtacg gtgtagcccg cgtagatatg agactcccaa
    10621 cgtacgccaa aaccgccagg tgcgtggaaa cgggtgattt tgcctgggct tggcaggaag
    10681 gtgttcggat cttcggcgtt gatacgacat tccaccgcat ggccgcgaac gtgaacttct
    10741 tcttgcttga tcgacagcgg ttgaccggca gcgatacgga gctgttcttt gatcaggtca
    10801 acgccagtga tcatttcggt aaccgggtgt tctacctgaa tacgggtgtt catttcgatg
    10861 aaatagaact cgccgttttc gaacagaaac tcgaaagtac ctgcaccgcg atagccgata
    10921 tcaacacacg ctttagcgca acgttcgccg atgtagcgac gcagttccgg ggtaatgccc
    10981 ggtgctggcg cttcttcgac cactttctgg tggcggcgct gcatggagca gtcacgttcc
    11041 gccagataga tagcgttgcc ccgaccgtca gccagtacct gaatctcgac gtggcgagga
    11101 ttttccaggt atttctccat gtaaaccata tcgttgctga aagcagcttt cgcttccgca
    11161 cgggtcatgg agatggattg tgccagttca gcgtcgccgc gcactacgcg cataccgcga
    11221 ccaccgccgc cgccggaggc tttgataatc accggataac caatgcgttt agcaatggca
    11281 cggtttttat ccatatcgtc gcccaacggg ccgtcagaac ccggtacgca agggacgccc
    11341 gcttttttca tcgccgcgat tgcggatact ttgtcgccca tcaggcgaat ggtttctgct
    11401 ttcgggccga tgaagataaa gccggagcgt tcaacctgct cggcaaagtt ggcgttctcg
    11461 gagaggaagc cgtaacccgg atggattgct actgcgccgg tgatttcagc ggcgctgatg
    11521 attgccggga tgttcagata actttttact gacggagcag ggccaataca gaccgtttca
    11581 tctgccagta atacgtgttt tagatcgcga tccgcgctgg agtgcacagc gacagtcttg
    11641 atgcccagtt ctttacaggc acgaagaata cgcaatgcaa tctcgccgcg gttggcaata
    11701 acaattttat ccagcatgtt cgcctcgtta ctcgatgacg accagcggct cgtcaaattc
    11761 taccggttgt ccactttcga ccagaattgc tttcacggta ccggatttgt ccgcttcgat
    11821 ctggttcatc attttcatgg cttcaacgat gcacagggta tcgcccacgt tgactttctg
    11881 acccacttcg atgaacgctt ttgcgtccgg gcttggggtg cggtagaaag taccaaccat
    11941 cggggaacgt acgatgtgac cactgatttc cgctgctgct ggcgcttcca tggaaggaac
    12001 ggtcgccgga gcggctgcgt tagattgagc tggctgctgc atcattggtg cagcgtaagc
    12061 ttgttgcatc acagggaaac ttgcggcagg agctgcacgg ctaatgcgta ctgactcttc
    12121 gccttcagaa atttccagtt cggagatgcc tgattcttca accagctcga tcagtttttt
    12181 aatcttacga atatccatga gtgggttccg tactctttgt ttagtgtgat tgtgacaggc
    12241 gtttcaccgc cgtctgtaaa gcgtatgccc cgacagctgt atgcatagcg ataaattcca
    12301 gcaggccggg acgccggtct attttgcccc agagtatcaa tgttagactt gacggacatt
    12361 gtgcagcgat ctgctgctat cctccggcaa aaaacaaaat ataccttcgt tgcgccaatg
    12421 tcaccttttc cgcagcgcaa aaactgcgtc agggaggacg aggtcgcaca ttataacgat
    12481 ttcgtagcaa ttggcagcta aatactggtc tcatcaggga agataatcaa cagctaacat
    12541 gtaaataacc ttcaacaccg tgtaatttgc aacaagccgc acaattcacg aaattagcgc
    12601 catcatcgac ggaacttctt ataacgtaag gtgaaaagct gtaaagccag ccctgcgaag
    12661 atgaggggct gcaatgagat aatcttcaca gaccacaaat aatgtaggga tgccaggatc
    12721 gcgacaagat agacgacatt acgcagcggt tgctggggat tacccaattt tcactgcatc
    12781 gtctggatag atgtgaatgc taaagcacgc aaaatcaacc gactgacaat tctcagcatt
    12841 aaataggttc gagtggttaa tttgtagtca aataaaatca gattattcac cccatggtac
    12901 cagaaatcag taacaggtta agcgtagtgt cgcccaggca aagcaccata aacctaacag
    12961 gcgacggagg cgtatcaata atggctgttt agaggagtat caatgcgcat caatttgtgg
    13021 tcagtcgaaa tcatgacttt ccagtttgga tgatgttgta caacttcctg accaaggtgt
    13081 tcgacgaaag ctgtccggct aaccgctttt cccgttaacc gattgaataa tgatgtcaaa
    13141 atgagttccc tctcttatta ttccttgcta aatggttagt taaccttcac cagcgtgcga
    13201 ccctggatct ggttattaat gatggcctcg gcaaagttcg gtgcctctga cagagatatc
    13261 tcttttgccg cctgggtata gaatgattcc ggtaaatcgg cgaccagtcg ctgccaggct
    13321 tgtgcgcggc gttctggtgg cgtcattact gaatccaccc cttgcaaacg gacattacgc
    13381 agaataaatg gcatgaccgt ggttggcaga gtaaaaccac ccgccagacc acaggccgcc
    13441 acgcagccgc cgtaattcat ttgcgccagc acttttgcca gcactttgtc gccaacagtg
    13501 tcaattgccc cagcccagac ttgtttttcc agaggacggg attcggcaaa ctcatcacga
    13561 gggagaatac ggctggcacc taaacttttc agatattcat gggtactttc gcgaccggaa
    13621 acggcaacga cctgataacc caacttatgc agcagcgcca cggcggtact gccgacgcca
    13681 ccactggcac ccgtcacgac aatctccccg tcctgcgggc gaacaccggc atcttccagc
    13741 gccatcacac acagcatggc ggtaaaaccg gcagtaccga taatcattgc tttacgcgcg
    13801 tccagccctt gcggcatggc aaccagccag tcacctttca ctcgcgcctg ctccgccagc
    13861 ccaccccagt ggttttcacc aacgccccag ccagtgagta acacctcctg accggcatga
    13921 aaacgcggat cttcgctggt gcgtacagtt ccggcaaaat cgatcccagg aatcatcgga
    13981 aaattacgga tgatttttcc cttaccggta atcgccagcg catctttata gttcaggctc
    14041 gaccagtgaa catcgaccgt gacatcgccc tccggcaggc gactttcgtc cagagtctgt
    14101 actgatgcga gagttttgcc gtcctgctgt tctaaaagta acgcctgcat aagtggtcct
    14161 catgtgcatg atggattgga aaataatttc tgaagattat actcgctaat ggaaaagcaa
    14221 agccgatgaa gcgcaataaa ttgcgagata aatctgattt gctagtatgc ccgcttcctc
    14281 actatcggag ttaacacaag gatgagatta acgacgaaat tttcggcctt tgttacgctg
    14341 ctcaccgggt taacaatttt tgtgactttg ctgggctgtt cgctaagttt ctacaacgca
    14401 attcagtata agtttagtca tcgcgttcag gcggtggcga cggcgattga tacccacctt
    14461 gtgtcgaatg acttcagcac attaaggcca caaattaccg aattaatgat gtcggcagat
    14521 atcgttcgtg tagacctgct ccatggtgat aaacaggttt ataccctggc cagaaatggt
    14581 agttatcgtc cggttggctc cagcgatctg tttcgcgaac tgagtgttcc gttgataaag
    14641 catccgggga tgtcgctgcg tctggtttat caggatccga tgggcaacta tttccattct
    14701 ttaatgacta ccgcgccgct cacgggggcg attggcttta tcattgttat gctcttcctg
    14761 gcggtacgct ggttacaacg gcaacttgcc gggcaagaat tgctggaaac ccgggctact
    14821 cgtatcttaa acggtgagcg tggctctaat gtgttgggaa ccatctatga atggccgccc
    14881 agaaccagca gtgcgctaga tacgctgctt cgtgaaattc agaacgcacg cgaacaacac
    14941 agccgtcttg atacgctgat ccgctcttat gccgcccagg acgtgaaaac cggcctcaat
    15001 aaccgactct ttttcgataa tcagttagca acgttactgg aagatcagga gaaagtaggt
    15061 acccacggga tcgtgatgat gattcgtctg ccggatttca atatgttgag cgatacttgg
    15121 gggcacagcc aggttgaaga acagttcttc actctgacta atctgctgtc gacatttatg
    15181 atgcgctacc ctggcgcact gctggcgcgt taccaccgca gtgattttgc tgcgctgtta
    15241 ccgcaccgga cgttaaaaga ggcagagagc atcgccggtc agttaatcaa agccgtcgat
    15301 accttgccga acaataaaat gctcgatcgc gacgatatga tccacattgg tatctgcgcc
    15361 tggcgtagtg gtcaggatac cgagcaggta atggaacatg cagagtctgc cacgcgtaat
    15421 gcgggattgc agggcggcaa tagctgggct atttacgatg actcgttgcc tgaaaaagga
    15481 cgcggtaatg ttcgctggcg tacgcttatc gagcaaatgc tcagtcgcgg cggcccgcgc
    15541 ctttatcaaa aaccggcggt tactcgcgaa ggtcaggttc atcatcgcga actcatgtgc
    15601 cgcatcttcg atggtaatga agaggttagc tcggcggagt atatgccgat ggtcttgcag
    15661 tttggcttat cggaagagta tgaccgtctg caaatcagcc gtcttattcc actattgcgt
    15721 tactggccag aggaaaatct ggcgattcag gttaccgttg agtcgctgat tcgcccgcgt
    15781 tttcagcgtt ggctgcgcga tacgttaatg caatgtgaaa aatcacaacg aaaacgcata
    15841 attattgaac ttgcagaggc cgatgtaggt caacatatca gtcgtttaca acctgttatt
    15901 cgtttagtga atgctttagg ggtacgggta gccgtcaacc aggctggttt gacgctggta
    15961 agcaccagtt ggatcaaaga acttaatgtt gagttactca agctccatcc ggggctggtc
    16021 agaaacattg agaagcgaac ggagaaccag ctgctggttc aaagcctggt ggaagcctgc
    16081 tccgggacca gcacccacgt ttacgccacc ggcgtgcgtt cgcgaagcga gtggcagacc
    16141 ctgattcagc gcggtgttac aggcgggcaa ggggattttt tcgcgtcctc acagccactt
    16201 gatactaacg tgaaaaaata ttcacaaaga tactcggttt aacctgccgt ttaatccgtt
    16261 ttcacgtaga ataacgcgcg ctgcgtctca tgggagtgtg cttgtctgct cgccagattg
    16321 ttgcagcaca tatgcagatg aatgacctta cgcggttgca aacaggcgag gaatgctgct
    16381 gatgcattaa gcctttctgg actcaggcag agatttgtaa caaaggaaac gaactgcact
    16441 aattttcacc gtagcagatg atttttgcgc cttgtcgctg ctgcgtgtgg ttggtaaagt
    16501 aagcggattt tcttttccgc cccagctttc aggattatcc cttagtatgt tgaaaaaatt
    16561 tcgtggcatg ttttccaatg acttgtccat tgacctgggt actgcgaata ccctcattta
    16621 tgtaaaagga caaggcatcg tattgaatga gccttccgtg gtggccattc gtcaggatcg
    16681 tgccggttca ccgaaaagcg tagctgcagt aggtcatgac gcgaagcaga tgctgggccg
    16741 tacgccgggc aatattgctg ccattcgccc aatgaaagac ggcgttatcg ccgacttctt
    16801 cgtgactgaa aaaatgctcc agcacttcat caaacaagtg cacagcaaca gctttatgcg
    16861 tccaagcccg cgcgttctgg tttgtgtacc ggttggcgcg acccaggttg aacgccgcgc
    16921 aattcgtgaa tccgcgcagg gcgctggtgc ccgtgaagtc ttcctgattg aagaaccgat
    16981 ggctgccgca attggtgctg gcctgccggt ttctgaagcg accggttcta tggtggttga
    17041 tatcggtggt ggtaccactg aagttgctgt tatctccttg aacggcgtgg tttactcctc
    17101 ttccgtgcgc attggtggtg atcgtttcga cgaagctatc atcaactatg tgcgtcgtaa
    17161 ttacggttct ctgatcggtg aagccaccgc agaacgtatc aagcacgaaa tcggttcggc
    17221 ttacccgggc gatgaagtcc gtgaaatcga agttcgtggc cgtaacctgg cagaaggtgt
    17281 tccacgcggt tttaccctga actccaacga aatcctcgaa gcactgcagg aaccgctgac
    17341 cggtattgtg agcgcggtaa tggttgcact ggaacagtgc ccgccggaac tggcttccga
    17401 catctccgag cgcggcatgg tgctcaccgg tggtggcgca ctgctgcgta accttgaccg
    17461 tttgttaatg gaagaaaccg gcattccagt agttgttgct gaagacccgc tgacctgtgt
    17521 ggcgcgcggt ggcggcaaag cgctggaaat gatcgacatg cacggcggcg acctgttcag
    17581 cgaagagtaa tcggatgcag gcaggggaaa tgtctgttta ccctgcctgg tctgatacga
    17641 gaatacgcat aacttatgaa gccaattttt agccgtggcc cgtcgctaca gattcgcctt
    17701 attctggcgg tgctggtggc gctcggcatt attattgccg acagccgcct ggggacgttc
    17761 agtcaaatcc gtacttatat ggataccgcc gtcagtcctt tctactttgt ttccaatgct
    17821 cctcgtgaat tgctggatgg cgtatcgcag acgctggcct cgcgtgacca actagaactt
    17881 gaaaaccggg cgttacgtca ggaactgttg ctgaaaaaca gtgaactgct gatgcttgga
    17941 caatacaaac aggagaacgc gcgtctgcgc gagctgctgg gttccccgct gcgtcaggat
    18001 gagcagaaaa tggtgactca ggttatctcc acggttaacg atccttatag cgatcaagtt
    18061 gttatcgata aaggtagcgt taatggcgtt tatgaaggcc agccggtcat cagcgacaaa
    18121 ggtgttgttg gtcaggtggt ggccgtcgct aaactgacca gtcgcgtgct gctgatttgt
    18181 gatgcgaccc acgcgctgcc aatccaggtg ctgcgcaacg atatccgcgt aattgcagcc
    18241 ggtaacggtt gtacggatga tttgcagctt gagcatctgc cagcgaatac ggatattcgt
    18301 gttggtgatg tgctggtgac ctccggtctg ggcggtcgtt tcccggaagg ctatccggtc
    18361 gcggttgtct cttccgtaaa actcgatacc cagcgcgctt atactgtgat tcaggcgcgt
    18421 ccgactgcag gactgcaacg tttgcgttat ctgctgctgc tgtggggcgc ggatcgtaac
    18481 ggcgctaacc cgatgacgcc ggaagaggtg catcgtgttg ctaatgaacg tctgatgcag
    18541 atgatgccgc aggtattgcc ttcgccagac gcgatggggc caaagttacc tgaaccggca
    18601 acggggatcg ctcagccgac tccgcagcaa ccgacgacag gaaatgcagc tactgcgcct
    18661 gctgcgccca cacagcctgc tgctaatcgc tctccacaaa gggctacgcc gccgcaaagt
    18721 ggtgctcaac cgcctgcgcg tgcgccggga gggcaatagt ggcgagctat cgtagccagg
    18781 gacgttgggt aatctggctc tctttcctca ttgcgctgtt gctgcaaatc atgccctggc
    18841 cggataacct gattgttttc cggccaaact gggtgttact catcttgttg tattggatcc
    18901 tggccttgcc tcatcgcgta aatgtgggca caggttttgt gatgggtgcc atactggatc
    18961 tgatcagcgg ctcgacgctt ggcgtacgcg tattggcgat gagcatcatt gcttacctgg
    19021 tggcgctgaa ataccagctt ttccgcaacc tcgcattatg gcagcaggcg ctggtcgtca
    19081 tgttgctttc gctggtggtg gatattattg ttttctgggc agagttttta gtgattaacg
    19141 tctctttcag accggaagtg ttctggagta gtgtagtcaa tggggtgctc tggccgtgga
    19201 ttttcttgct gatgcgtaaa gtccgtcagc agtttgcagt gcaataaagg tttctatgac
    19261 ttctctgtat ttagcttccg gttctccgcg tcgtcaggag ttacttgcgc aacttggtgt
    19321 gacctttgaa cgtattgtta cgggcattga ggagcagcgt cagccgcagg agagcgcgca
    19381 gcagtatgtt gtgcgtctgg cgcgcgagaa agcacaggcg ggtgtcgcgc aaacggcgca
    19441 ggatctcccg gtgctgggtg cggatactat cgttatcctg aatggagaag tgctggagaa
    19501 accgcgcgac gcagagcatg cggcgcagat gttgcgcaaa ttatcgggtc agacccatca
    19561 ggtgatgacg gcagtggcgt tggccgacag ccagcacatt ctcgattgcc tggtggtcac
    19621 cgatgtgact ttcagaacgt taacagacga agacatcgcg ggctatgtcg ccagcggtga
    19681 accgttagat aaagcaggtg catacggtat tcaggggctg ggtggctgtt ttgtcaggaa
    19741 gataaatggc agctatcacg ccgtagtcgg cttacctctg gttgaaacgt atgaattatt
    19801 aagtaatttt aacgcactgc gtgagaaaag ggataaacat gacggctgaa ttgttagtaa
    19861 acgtaacgcc ttcggaaacg cgagtggcgt atattgatgg cggtattctg caggaaattc
    19921 atattgaacg tgaggcgcga cgcggaatag taggcaatat ctacaagggt cgtgtaagtc
    19981 gtgtacttcc gggtatgcag gcggcttttg tagatattgg gctggataaa gccgcgtttc
    20041 ttcatgcatc cgatatcatg ccgcacaccg aatgtgtggc gggtgaagaa caaaagcaat
    20101 tcacggtgcg cgacatctcg gaactggttc gtcaggggca agatctgatg gtgcaggtgg
    20161 tgaaagatcc gcttggcact aaaggtgcgc gcctgaccac cgatatcacg ctgccttctc
    20221 gctatctggt ctttatgcca ggggcttctc acgttggggt ttcccaacgt attgaaagcg
    20281 aatcagaacg tgaacgcctg aaaaaagtgg tcgcagagta ttgcgacgag cagggcgggt
    20341 ttatcatccg taccgcagcg gaaggggttg gcgaggctga actggcctcc gatgccgctt
    20401 atctgaaacg cgtctggacc aaagttatgg agcgtaaaaa gcgcccgcag acccgttatc
    20461 agctgtacgg cgaactggcg ctggctcagc gtgttctgcg tgatttcgcc gacgccgaac
    20521 tggaccgcat tcgcgttgac tcacgcctga cttacgaagc attacttgag ttcacctcgg
    20581 agtacattcc cgagatgacc agcaagctgg agcattacac cggacgccag ccgattttcg
    20641 atctctttga tgtcgaaaac gaaatccagc gagcgctgga acgcaaagta gaactgaaat
    20701 ccggtggtta tctcattatc gaccagaccg aagcgatgac caccgtggac atcaataccg
    20761 gagcgtttgt cggtcatcgc aatctggacg acaccatttt caataccaat attgaagcga
    20821 cgcaggctat cgctcgccag ttacggttgc gtaatctggg cgggattatc attattgatt
    20881 tcatcgatat gaataatgaa gatcaccgcc gccgcgtgct gcactcgctg gagcaggcgt
    20941 tgagcaaaga ccgggtgaaa accagcgtta atggtttttc ggcgctgggg ctggtggaga
    21001 tgacgcgtaa acgcacccgc gaaagcattg agcacgtact gtgtaacgaa tgcccaacct
    21061 gccacggtcg cggaacggtg aaaaccgtgg aaacggtatg ctatgaaatc atgcgcgaga
    21121 ttgttcgtgt ccaccatgct tacgactccg accgtttcct ggtctatgct tctccggcag
    21181 tagccgaagc cttgaaaggc gaagagtcac actcgctggc ggaagtggaa attttcgttg
    21241 gcaaacaggt taaagtacaa attgaaccgc tctataacca ggagcagttt gacgtcgtaa
    21301 tgatgtaaac agatgctggg ccgccatccg gcaaagggtt tttgagtcac atttttagca
    21361 gacaaggagt gacgggtgag gcgattgccg gggattttac tgcttactgg agccgcgctc
    21421 gttgtgatcg ctgccctgct ggttagcggc ctgcgtattg ctttaccgca tcttgacgcc
    21481 tggcgtccgg aaatcctcaa caaaatagaa tccgcgactg gcatgccggt agaagccagt
    21541 cagctctcag ccagctggca gaattttggc ccgacgcttg aagcacacga catccgtgca
    21601 gaactaaaag atggcggcga attttcggtt aaacgcgtta ctctggcgct ggatgtctgg
    21661 cagagcctgt tacatatgcg ctggcagttt cgcgacctca ctttctggca gctgcgcttt
    21721 cgcaccaaca ctcctatcac cagcggtggt agcgatgaca gtctggaagc cagtcacatc
    21781 agcgatctgt ttcttcgtca atttgaccat ttcgatctcc gcgacagtga agtcagtttc
    21841 ctgacgccat ccggtcagcg tgccgagctg gcgatcccac aactcacctg gctgaacgat
    21901 ccacgtcgac accgtgcgga aggcctggta agcctctcca gccttaccgg acagcacggc
    21961 gtgatgcagg tgcgcatgga tttgcgcgat gatgaggggt tgttaagcaa tggtcgcgtc
    22021 tggctacagg cggatgacat cgacctgaag ccgtggctcg gtaaatggat gcaggacaat
    22081 attgctctgg aaacggcaca gttctccctt gaaggctgga tgacgatcga caaaggcgat
    22141 gtaaccggcg gtgacgtctg gctgaaacag ggcggtgcca gctggttggg cgagaagcaa
    22201 acgcatacgc tgtcggtgga taatctgacc gcgcatatta cgcgtgaaaa tccgggctgg
    22261 cagttctcta ttcccgatac acggatcacg atggacggca aaccctggcc gagcggagca
    22321 ttgacgctgg cctggatacc ggaacaggac gttggcggca aagacaataa acgcagtgac
    22381 gaactccgga ttcgcgccag taatctggag ctggcaggcc tggagggcat acgcccgctg
    22441 gccgcgaaac tttcacctgc actgggtgat gtttggcgct ccacacaacc gagcggcaag
    22501 attaacactc tggcgctgga tatcccgctt caggcggcag acaagacccg ttttcaggca
    22561 tcgtggagcg atctggcctg gaagcaatgg aaattattac cgggtgcgga acacttctcc
    22621 gggacgcttt ccggcagcgt tgaaaatggt ttgcttaccg cgtcgatgaa gcaggcaaag
    22681 atgccttacg aaacggtatt ccgtgcgcca ctggaaatcg ccgacggcca ggcaactata
    22741 agctggctga acaatgacaa aggtttccag ctggatgggc gtaatattga cgttaaagcc
    22801 aaagccgtcc atgcgcgcgg cggttttcgt tacctgcaac ctgctaacga tgaaccctgg
    22861 ctgggtattc tggctggcat cagtaccgat gatggttcac aagcctggca ctatttcccg
    22921 gaaaacttga tgggtaaaga cctggttgat tacttaagtg gcgcgattca gggcggtgaa
    22981 gcggataacg cgacgctggt ttatggtggc aatccgcaac tcttccccta taaacacaac
    23041 gaaggtcagt ttgaagtgct ggtgccgctg cgcaacgcga agtttgcctt ccagccggac
    23101 tggcctgcat taactaacct tgatattgaa ctggacttta ttaacgacgg tttatggatg
    23161 aaaaccgatg gcgttaatct gggcggcgtg cgcgcgagta atctcaccgc agtgatccct
    23221 gactactcga aagaaaaact gctgattgac gctgacatta aaggtccggg taaagccgtt
    23281 ggcccttact ttgatgagac accgctgaaa gattctctgg gtgcgaccct gcaagaactc
    23341 cagctcgacg gcgatgtgaa tgctcgctta catcttgata tcccgctgaa cggcgagctg
    23401 gtaaccgcga aaggtgaagt gacgctgcgt aataacagtc tgtttatcaa accactcgac
    23461 agcaccctga aaaatttgag cggtaaattc agctttatca atggcgatct gcaaagtgaa
    23521 ccactgacag caagctggtt taatcagccg ttgaacgtgg atttttccac caaagaaggg
    23581 gcaaaagcct accaggttgc ggtgaatctc aacggtaact ggcaaccggc gaaaaccggc
    23641 gttctgcctg aagcggtgaa cgaagcattg agtggcagcg tggcgtggga tggtaaagtg
    23701 ggcattgatc tgccttatca tgctggcgcg acctataacg tagagctgaa cggcgatctg
    23761 aataatgtga gtagtcactt accttcaccg ttagccaaac ctgcgggtga accactagcg
    23821 gtaaacgtta aggttgatgg caatctcaac agctttgaat taaccggaca ggctggtgcg
    23881 gataaccatt tcaatagccg ctggttgctt ggtcaaaagc tgacgctcga tcgtgctatt
    23941 tgggcggcag acagtaaaac gctcccgccg ttgccggaac aaagtggcgt tgaactcaat
    24001 atgccgccga tgaatggtgc cgagtggctg gccctgttcc agaaaggcgc tgcggagagt
    24061 gtcggtggtg cagcgagttt cccacaatac ataacgttac gtacgcctat gttgtcactg
    24121 ggaaatcagc aatggaataa cctgagtatt gtttcgcaac cgacggcaaa tggcaccctg
    24181 gttgaggcgc aagggcgtga aatcaacgcc acgctagcga tgcgtaataa cgcgccgtgg
    24241 ctggcgaata tcaaatatct ttattacaac ccgagcgtgg cgaaaactcg tggtgattca
    24301 acgccgtcat cacctttccc gacaacggag cgcattaact tccgtggctg gccggacgca
    24361 caaatacgat gcacagagtg ctggttctgg gggcaaaaat tcggtcgcat tgacagtgat
    24421 atcaccattt ctggcgatac gttaacgctg accaatggac tgattgatac tggtttctcg
    24481 cggcttactg ccgatggtga atgggttaat aatccgggga atgaacgtac ctcgctgaaa
    24541 ggaaaactgc gcgggcagaa aattgatgcc gccgcagaat tttttggtgt cacgacgccc
    24601 atacgccagt cgtcatttaa tgtggattac gatttacact ggcgcaaagc accctggcag
    24661 ccagatgaag cgacgttgaa tggcatcatt catactcaac tgggtaaagg cgaaattacc
    24721 gaaatcaata ccggacatgc cgggcaattg ctgcgcttat tgagcgtaga tgccctgatg
    24781 cgtaagctgc gttttgattt cagagacact tttggcgaag ggttctattt tgactccatt
    24841 cgcagcaccg cgtggattaa agacggggtt atgcacaccg acgacacgct ggtggatggc
    24901 ctggaggcgg atatcgccat gaaagggtcg gtaaatctgg tacgtcgcga cctgaatatg
    24961 gaagcggttg tcgcaccaga gatttctgcg acggtgggcg tggctgcggc ttttgcggtt
    25021 aaccccattg ttggcgcggc agtttttgcc gctagtaaag tgctggggcc gctgtggagc
    25081 aaagtctcca ttttgcgcta tcacatttcg ggtccgctgg acgatccgca aatcaacgaa
    25141 gtgttgcgcc aaccgcgtaa agaaaaagcg caatgatttg acgagggcgc gtaattgccc
    25201 caatctcata tgataatcgt tgccaaaggc caacgagcca gaacataacc gtaggccgga
    25261 tagggcgttc atgccgcatc cggcagccgt aaaaaatcct ctactgcagt aactaacgag
    25321 tagcaaaaac gatgagtctg aacctggtaa gtgaacaatt gctagcggcg aacagcctga
    25381 aacatcagga cttgttcgcg atcctcggtc aactggccga acgtcgcctt gattatggcg
    25441 atctctattt tcagtcgagc tatcacgaat cctgggtttt agaagaccgc attattaaag
    25501 atggttctta caacatcgat cagggcgttg gcgtgcgtgc aatcagcggt gaaaaaaccg
    25561 gatttgctta cgctgaccaa atcagcctgc tggcgctgga acagagtgcg caagcggcgc
    25621 gcaccatcgt ccgtgatagt ggtgatggca aagtacagac gctgggcgcg gtagagcata
    25681 gcccgttgta tacctcggta gatccgctgc aaagcatgag ccgtgaagag aagctggata
    25741 tcctgcgtcg cgtcgataag gttgctcgcg aagcggacaa gcgcgtacag gaagtgactg
    25801 ccagcctcag tggtgtctat gaattaattc tggttgcggc caccgacggc acgctagcgg
    25861 cggatgtccg tccgctggtg cgtctttccg tgagcgttct ggtcgaagaa gatggcaaac
    25921 gcgaacgcgg tgccagtggc ggcggcggtc gttttggtta tgagttcttc cttgccgatc
    25981 ttgacggcga agtccgtgcg gatgcatggg caaaagaagc agtgcgtatg gcgctggtca
    26041 atctttctgc cgttgctgca ccagcgggca ccatgccggt agtacttggc gcaggttggc
    26101 cgggcgtgct gttgcatgaa gcggtaggtc acggtctgga aggcgacttc aaccgccgcg
    26161 gcacttcagt atttagtggg caggtcgggg agctggtggc ttcagaactg tgtaccgtgg
    26221 ttgatgacgg cacgatggtc gatcgccgtg gttcggtggc gattgatgac gaaggtacgc
    26281 caggccagta caacgtgctg attgagaacg gcattctgaa aggctacatg caggataaac
    26341 tcaacgcgcg tttgatgggg atgacgccga ctggcaacgg tcgccgtgaa tcctacgccc
    26401 atctgcccat gccgcgtatg actaacacct atatgctgcc gggtaaatcg accccgcagg
    26461 aaattattga atccgttgag tacggtatct atgcaccgaa ctttggtggc ggtcaggtgg
    26521 atatcacctc cggcaaattc gttttctcca cttcagaagc atatctgatt gaaaacggta
    26581 aagtaacgaa gccggtgaaa ggcgcaacgt tgattggttc cggtatcgaa accatgcagc
    26641 agatttcgat ggttggcaac gacctgaaac tggataacgg cgtgggtgtc tgcggtaaag
    26701 aagggcaaag tttgccggtt ggcgtgggcc agccaacgct gaaagttgat aacctgactg
    26761 ttggcggtac tgcgtaataa tttcattatt ttcagaagga taattaatct ttctacgtgc
    26821 acgaacggtc ccctcgctcc tctggggtta gggtgagggg aacccgttgg cacaggtttg
    26881 tagaacgtaa cagtacaata tgaattactt ctctttcccg cgcccgtgca tctcctgaaa
    26941 caatttaccg acctcaacaa aataatccgt cagcgagttg atcacgacct gtaccttcag
    27001 cggcagctta tctttttcgg tatataacgc ataaaccggg cgtggatctg actgataacg
    27061 cggcagcagg atctccagct caccgcgatt gatctcgttg atcacccaca tcagcggcac
    27121 gtaggcgatc ccggcacccg ccgtcagcca gcgcaccagc gtcatcggat cattagtcac
    27181 aaatcttcct tgtgggatca ggcgagtcga gatcccttcc ggtgcgatca gttcaaattc
    27241 attgtcgggc cgcacgctgt attcaagcca tgaatgacta ctcaaatcgg cgggtttttc
    27301 cggtatgccg tattgtgtga gatagctttt cgcggcgcac accaccatcg gcatcgcgcc
    27361 cagacggcgg gaaaacaggc tggaattccg gtaaccagat tgacgctcaa acctgggtat
    27421 tctttcagca tttttgctgt cagcccggcg agaacatttt gtgccatagt tgaagaacag
    27481 ccaatgcgca gcgtcccgat gggggtgtta ttgaaggcat acagttgctc atgaacatcc
    27541 tgcacttcat gaagcatccg acggcagccc tggtagtaaa ttctgccggc ttcggtcagg
    27601 ccaatgctgc gtgtgctacg gtttaacagc tttacctgca actcatcttc cagttttgac
    27661 accgtctgac tgatggacga aacgctcatc tgtagctgtc tggcggcggc ggtaaaagag
    27721 ccaaattcaa ctactttggc aaacaccgac atgcgtttta gtcgttccat tattcactct
    27781 gacttaaaag tgatttagat cacataatat agataacagc ataacagtta cgctaatata
    27841 ttaaatatca atctacagca atgttgctct cgcccggctt tccatgccct tctctgcggc
    27901 gacagatgct gaaaataata acgcctgctc tctctttaca accaaggtca acatgagtct
    27961 gtttcccgtt atcgtggtgt ttggactgtc cttcccaccg atattttttg aattgctttt
    28021 atcactggcg attttctggc tggtgcgccg ggtacttgtg ccaacaggta tctacgactt
    28081 tgtctggcat ccggcgttgt tcaacaccgc gctctattgc tgcttgtttt atttgatatc
    28141 gcgactgttc gtttgaggtt gaagtgaaaa cactaataag aaaattctcc cgtacggcca
    28201 tcacggtcgt attagtcatt ctagccttca tcgcaatttt taatgcctgg gtctattaca
    28261 ccgaatcccc ctggacgcgt gacgcgcgct ttagcgctga cgtcgttgcg atcgcgccgg
    28321 acgtttctgg actcattacc caggtgaatg ttcatgataa ccagctggtg aaaaaaggac
    28381 agatactgtt caccatcgac cagccgcgct atcaaaaggc gcttgaggaa gcgcaagccg
    28441 atgttgctta ttatcaggta ctggcacagg agaaacgcca ggaggccgga cgtcgtaacc
    28501 gtctcggtgt gcaggcgatg tctcgcgaag agatcgacca ggccaacaac gtactacaaa
    28561 cggttctgca tcagttagcg aaagcgcagg cgacccgcga tctggcaaaa ctggatcttg
    28621 aacgcacggt gatccgcgcg ccagcagatg gctgggtgac caacctcaac gtctataccg
    28681 gtgagtttat tactcgagga tcaacggcgg ttgcgctggt gaaacagaac tccttctatg
    28741 tactggccta tatggaagaa actaagctgg aaggggggcg tccggggtat cgtgcagaga
    28801 tcacgccgct tggcagtaac aaagtgctga aagggactgt tgatagtgtt gccgcagggg
    28861 tcaccaacgc cagcagcacg cgtgacgaca aagggatggc gactatagac tctaatcttg
    28921 aatgggtgcg tcttgcgcaa cgtgttccgg ttcgtattcg tctcgacaac cagcaagaga
    28981 acatctggcc tgcgggcacc actgctacag tggtggtcac tggcaaacaa gatcgcgacg
    29041 aaagccagga ttcgttcttc cgtaaaatgg cccatcgcct gcgtgagttt ggttaatcac
    29101 gatgggtatt ttctccattg ctaaccaaca tattcgcttt gcggtaaaac tggcgaccgc
    29161 cattgtactg gcgctgtttg ttggctttca cttccagctg gaaacgccac gctgggcggt
    29221 actgacagcg gcgattgttg ccgccggtcc ggcctttgct gcgggaggtg aaccgtattc
    29281 tggcgctatt cgctatcgtg gctttttgcg catcatcggc acatttattg gctgtattgc
    29341 cggactggtg atcatcattg cgatgatccg cgcaccatta ttgatgattc tggtgtgctg
    29401 tatctgggcc ggtttttgta cctggatatc ctcgctggta cgaatagaaa actcgtacgc
    29461 gtgggggctg gccggttata ccgcgctgat cattgtgatc accattcagc cggaaccatt
    29521 gcttacgccg cagtttgccg tcgaacgttg tagcgagatc gttatcggta ttgtgtgtgc
    29581 gattatggcg gatttgctct tttctccgcg atcgatcaaa caagaagtag atcgagagct
    29641 ggaaagtttg ctggtcgcgc aatatcaatt aatgcaactc tgtatcaagc atggcgatgg
    29701 tgaagttgtc gataaagcct ggggcgacct ggtgcgacgc accacggcac tacaaggcat
    29761 gcgcagcaac ctgaatatgg aatcttcccg ctgggcgcgg gccaatcgac gtttaaaagc
    29821 gatcaatacg ctatcgctga cgctgattac ccaatcctgc gaaacttatc ttattcagaa
    29881 tacgcgcccg gaattgatca ctgatacttt ccgcgaattt tttgacacgc cggtagaaac
    29941 cgcgcaggac gtccacaagc agctcaaacg cctgcggaga gttatcgcct ggaccgggga
    30001 acgggaaacg cctgtcacca tttatagctg ggtcgcggcg gcaacgcgtt atcagcttct
    30061 caagcgcggc gttatcagta acacaaaaat caacgccacc gaagaagaga tcctgcaagg
    30121 cgaaccggaa gtaaaagtag agtcagccga acgtcatcat gcaatggtta acttctggcg
    30181 aaccacactt tcctgcattc tgggcacgct tttctggctg tggacgggct ggacttccgg
    30241 cagtggtgca atggtgatga ttgcggtcgt gacgtcactg gcaatgcgtt tgccgaatcc
    30301 acgcatggtg gcgatcgact ttatctatgg gacgctggcc gcgctgccgt tagggctgct
    30361 ctactttttg gtgattatcc ctaataccca acagagcatg ttgctgctgt gtattagcct
    30421 ggcagtgctg ggattcttcc tcggtataga agtacagaaa cggcgactgg gctcgatggg
    30481 ggcactggcc agcaccataa atattatcgt gctggataac ccgatgactt tccatttcag
    30541 tcagtttctc gacagcgcat tagggcaaat cgtcggctgt gtgctcgcgt tcaccgttat
    30601 tttgctggtg cgggataaat cgcgcgacag gactggacgt gtactgctta atcagtttgt
    30661 ttttgccgct gtttccgcga tgactaccaa tgtggcacgt cgtaaagaga accacctccc
    30721 ggcactttat cagcagctgt ttttgctgat gaataagttc ccaggggatt tgccgaaatt
    30781 tcgcctggcg ctgacgatga ttatcgcgca ccagcgcctg cgtgatgcgc cgatcccggt
    30841 taacgaggat ttatcggcgt ttcaccgaca aatgcgccgc acagcagacc atgtgatatc
    30901 tgcccgtagc gatgataaac gtcgtcggta ctttggccag ttgctggaag aactggaaat
    30961 ctaccaggaa aagctacgca tctggcaagc gccaccgcag gtgacggaac cggttcatcg
    31021 gctggcgggg atgctccata agtatcaaca tgcgttgacc gatagttaag tcaaaaccga
    31081 cgccaaaagc gtcggttttt tcatggctat acttagcgat gcacggcaga actcgccgcg
    31141 aaacgtgacg gtggcaacag atgaatattt atacctttga ttttgatgag attgagagtc
    31201 aggaggattt ttatcgtgac tttagccaaa cctttggtct ggctaaagat aaggtacgcg
    31261 atctcgactc actatgggat gtgttaatga acgatgtcct gccgctacca cttgagattg
    31321 aatttgttca tctgggagag aaaacgcgtc gccgttttgg cgcgttaata ttgctgtttg
    31381 atgaggcaga ggaagagctg gaagggcatt tgcgttttaa tgttcgtcat tagcgcaaaa
    31441 aaaagccccc gaaccggggg caatatcgtc ggacaagacg atgagggttt atttgtacag
    31501 ttcagccgta gcgtgccagg tgtcaccgct acgagcttca gtaatctggt aggccgttgc
    31561 gcctttctct tccgcttttt tgttcagcat ttcacgcata tccattggcg aagacgccac
    31621 accacttacg gatacggtcc cgattgcttc acgattttgt gcttgtgcag tatcaatgga
    31681 gtcggcagcg aatgcaccga aagagagaac agaaagtacg cttaatgcag ctacagtggt
    31741 tttgattttc atgattttta cctcgacata atcttttagc tgggcctttg tttcgtgatc
    31801 catatcacaa aatcaagcat acactaatta ttgaactaat taataccaca ctaactgttt
    31861 cagttgatac taagtgttaa aaatgtgaat tttaataaca aaaaagaatt aaaaacacgg
    31921 ttatttattg ttaattctag tattttcaat tagataatga attttacata atgtgctttt
    31981 tttaccgtat gtattcattg tgtgaatgat atgtcgcggt aaaacgcact attcgttaaa
    32041 ttaatcgcag gggaaagcag ggggttgaga ggaataagca acattttccc cgccgccaga
    32101 agcgacgggg cagagattaa agctcctggt cgaacagctc taaaatcgct tcgtacaggt
    32161 ctttgacggt gaaaccgtta gcaggggtgg taaagatggt gtcatcgcca gcgatggtgc
    32221 ccagaatacc ttctgctttg cccagtgagt ccagcaggcg agcaattaac tgcgccgcgc
    32281 cagggctggt atgaatcacg acaactgcat cgttgtagtc gatatccagc accagattct
    32341 tcaatggact ggaggtggtt ggtacaccca gttcagctgg caggcagtaa accatttcca
    32401 ttttggcatt gcgtgtacgt acagcaccaa acttggtcaa catccgcgag actttagact
    32461 gattaatatt gtcaaagcct tgctcctgca acgcggcgac gatttcgccc tgggagctaa
    32521 atttctcttc tttaagtaat gctttaaatg ctttaactag ttcttcttgc ttagccgagc
    32581 ttcgcataag tcacccgata tggtggttga tacaacatta ttgtgcatac agatgaattt
    32641 ttatgcaaac agtcagccct gaagaaggct gaaataatgt tatgaaagag cgggatttta
    32701 tcaaatttcg ttattgagaa acatgcctgc gtcacggcat gcaaattctg tttaaaagta
    32761 aattaattgt tatcaaattg atgttgtttt ggctgaacgg tagggtatat tgtcaccacc
    32821 tgttggaatg ttgcgctaat gcataagcga ctgttaatta cgtaagttag gttcctgatt
    32881 acggcaatta aatgcataaa cgctaaactt gcgtgactac acattcttga gatgtggtca
    32941 ttgtaaacgg caattttgtg gattaaggtc gcggcagcgg agcaacatat cttagtttat
    33001 caatataata aggagtttag gatgaaagtc gcagtcctcg gcgctgctgg cggtattggc
    33061 caggcgcttg cactactgtt aaaaacccaa ctgccttcag gttcagaact ctctctgtat
    33121 gatatcgctc cagtgactcc cggtgtggct gtcgatctga gccatatccc tactgctgtg
    33181 aaaatcaaag gtttttctgg tgaagatgcg actccggcgc tggaaggcgc agatgtcgtt
    33241 cttatctctg caggtgtagc gcgtaaaccg ggtatggatc gttccgacct gtttaacgtt
    33301 aacgccggca tcgtgaaaaa cctggtacag caagttgcga aaacctgccc gaaagcgtgc
    33361 attggtatta tcactaaccc ggttaacacc acagttgcga ttgctgctga agtgctgaaa
    33421 aaagccggtg tttatgacaa aaacaaactg ttcggcgtta ccacgctgga catcattcgt
    33481 tccaacactt ttgttgcgga actgaaaggc aaacagccag gcgaagttga agtgccggtt
    33541 attggcggtc actctggtgt taccattctg ccgctgctgt cacaggttcc tggcgttagt
    33601 tttaccgagc aggaagtggc tgatctgacc aaacgcatcc agaacgcggg tactgaagtg
    33661 gttgaagcga aagccggtgg cgggtctgca accctgtcta tgggccaggc agctgcacgt
    33721 tttggtctgt ctctggttcg tgcactgcag ggcgaacaag gcgttgtcga atgtgcctac
    33781 gttgaaggcg atggtcagta cgcccgtttc ttctctcaac cgctgctgct gggtaaaaac
    33841 ggcgtggaag agcgtaaatc tatcggtacc ctgagcgcat ttgaacagaa cgcgctggaa
    33901 ggtatgctgg atacactcaa gaaagatatc gccttgggcg aagagttcgt taataagtaa
    33961 ttgattagcg aataataaaa aaccggagca cagac
//
LOCUS       DAJTZL010000027        32530 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.027, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000027 DAJTZL010000000
VERSION     DAJTZL010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32530
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.027"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(134..1210)
                     /gene="glpQ"
                     /locus_tag="OO544_001112"
     CDS             complement(134..1210)
                     /gene="glpQ"
                     /locus_tag="OO544_001112"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCR6766761.1"
                     /translation="MKLKLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRGHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMDLNLVQLIAYTDWNETQQKQPDGSWVNYSYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTTDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(1215..2573)
                     /gene="glpT"
                     /locus_tag="OO544_001113"
     CDS             complement(1215..2573)
                     /gene="glpT"
                     /locus_tag="OO544_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HCR6766762.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFLYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            2846..4474
                     /gene="glpA"
                     /locus_tag="OO544_001114"
     CDS             2846..4474
                     /gene="glpA"
                     /locus_tag="OO544_001114"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HCR6766763.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            4464..5723
                     /gene="glpB"
                     /locus_tag="OO544_001115"
     CDS             4464..5723
                     /gene="glpB"
                     /locus_tag="OO544_001115"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HCR6766764.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVTDIHSGLESLRQQAPAHPYSLLEPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRVTPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            5720..6910
                     /gene="glpC"
                     /locus_tag="OO544_001116"
     CDS             5720..6910
                     /gene="glpC"
                     /locus_tag="OO544_001116"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HCR6766765.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGIGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRKIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            7103..8005
                     /locus_tag="OO544_001117"
     CDS             7103..8005
                     /locus_tag="OO544_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HCR6766766.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVKGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIEEISERSPLQKERLMTIAERLRQEGHQIGWQKGMHEQAIKIALRMLE
                     QGIDRDQVLAATQLSEADLAANNH"
     gene            complement(8046..8849)
                     /gene="yfaU"
                     /locus_tag="OO544_001118"
     CDS             complement(8046..8849)
                     /gene="yfaU"
                     /locus_tag="OO544_001118"
                     /EC_number="4.1.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxy-L-rhamnonate aldolase"
                     /protein_id="HCR6766767.1"
                     /translation="MNALLTNPFKERLRKGEVQIGLWLSSTTSYMAEIAATSGYDWLL
                     IDGEHAPNTIQDLYHQLQAVAPYASQPVIRPVEGSKSLIKQVLDIGAQTLLIPMVDTA
                     DQARQVASATRYPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESKTALDN
                     LDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAAGFLAV
                     APDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(8867..10156)
                     /locus_tag="OO544_001119"
     CDS             complement(8867..10156)
                     /locus_tag="OO544_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6766768.1"
                     /translation="MSTALLDAVVKKNRVRLIPFMLALYVLAFLDRSNIGFAKQTYQI
                     DTGLSNEAYALGAGIFFVVYAFLGVPANLLMRKLGARTWIGTTTLLWGFLSAAMAWAD
                     TEAKFLIVRTLLGAAEAGFFPGMIYLTSQWFPQRNRASIMGLFYMGAPLALTLGSPLS
                     GALLEMHGFMGHPGWFWMFVIEGLLAVGAGVFTFFWLDDTPEQARFLSKQEKTLLINQ
                     LASEEQQKVTSRLSDALRNGRVWQLAIIYLTIQVAVYGLIFFLPTQVAALLGTKVGFT
                     ASVVTAIPWVAALFGTWLIPRYSDKTGERRNVAALTLLAAGIGIGLSGLLSPVMAIVA
                     LCVAAIGFIAVQPVFWTMPTQLLSGTALAAGIGFVNLFGAVGGFIAPILRVKAETLFA
                     SDAAGLLTLAAVAVIGSLIIFTLRVNRTVAQTDVAHH"
     gene            complement(10213..11417)
                     /gene="rhmD"
                     /locus_tag="OO544_001120"
                     /pseudo
     CDS             complement(10213..11417)
                     /gene="rhmD"
                     /locus_tag="OO544_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311162.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnonate dehydratase"
     gene            complement(11432..12214)
                     /locus_tag="OO544_001121"
     CDS             complement(11432..12214)
                     /locus_tag="OO544_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HCR6766769.1"
                     /translation="MLESSKVPALTRAIDILNLIARIGPCSAATIIDTLGIPKSTAYL
                     LLNELRRQRFLSLDHQENFCLWTRLVELSGHALSKMDLRELARPRLTQLMDTTGLLCH
                     LGIIDNGSAYYILKVESSATISVRSHEGKSLSLYRSGIGKCLLAWQPAAVQQSIIEGL
                     VWEQATPTTITHPQQLHEELARIRRQGWSYDNGEDYPDVRCVAAPVFNANNELTAAIS
                     VVGTRLQINEEYRDYLAGKAIACARDISRLLGWKSPFDLQAS"
     gene            complement(12434..13636)
                     /locus_tag="OO544_001122"
     CDS             complement(12434..13636)
                     /locus_tag="OO544_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HCR6766770.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVRLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQSPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            complement(13736..14278)
                     /locus_tag="OO544_001123"
     CDS             complement(13736..14278)
                     /locus_tag="OO544_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6766771.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIKSYEEANAGARYTIMHPVSIEAGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
     gene            14557..14982
                     /gene="nudI"
                     /locus_tag="OO544_001124"
     CDS             14557..14982
                     /gene="nudI"
                     /locus_tag="OO544_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HCR6766772.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            complement(15021..15623)
                     /locus_tag="OO544_001125"
     CDS             complement(15021..15623)
                     /locus_tag="OO544_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCR6766773.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            15931..17070
                     /gene="arnB"
                     /locus_tag="OO544_001126"
     CDS             15931..17070
                     /gene="arnB"
                     /locus_tag="OO544_001126"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HCR6766774.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLAKLEHLNTHRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNRERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            17074..18042
                     /gene="arnC"
                     /locus_tag="OO544_001127"
     CDS             17074..18042
                     /gene="arnC"
                     /locus_tag="OO544_001127"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HCR6766775.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            18042..20024
                     /gene="arnA"
                     /locus_tag="OO544_001128"
     CDS             18042..20024
                     /gene="arnA"
                     /locus_tag="OO544_001128"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HCR6766776.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPAEKAFYGS
                     VARLAAERGIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGHRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQMLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NARRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            20021..20911
                     /locus_tag="OO544_001129"
     CDS             20021..20911
                     /locus_tag="OO544_001129"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HCR6766777.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            20911..22563
                     /gene="arnT"
                     /locus_tag="OO544_001130"
     CDS             20911..22563
                     /gene="arnT"
                     /locus_tag="OO544_001130"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HCR6766778.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFASLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGFTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            22560..22895
                     /gene="arnE"
                     /locus_tag="OO544_001131"
     CDS             22560..22895
                     /gene="arnE"
                     /locus_tag="OO544_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HCR6766779.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            22895..23281
                     /gene="arnF"
                     /locus_tag="OO544_001132"
     CDS             22895..23281
                     /gene="arnF"
                     /locus_tag="OO544_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HCR6766780.1"
                     /translation="MGLMWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(23275..23541)
                     /gene="pmrD"
                     /locus_tag="OO544_001133"
     CDS             complement(23275..23541)
                     /gene="pmrD"
                     /locus_tag="OO544_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HCR6766781.1"
                     /translation="MEWLVKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCKVAGKTQ"
     gene            complement(23585..25006)
                     /gene="menE"
                     /locus_tag="OO544_001134"
     CDS             complement(23585..25006)
                     /gene="menE"
                     /locus_tag="OO544_001134"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HCR6766782.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQRVEGAHAATWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     DYEIISFTSQFCHSIAVDNRFIC"
     gene            complement(25003..25965)
                     /gene="menC"
                     /locus_tag="OO544_001135"
     CDS             complement(25003..25965)
                     /gene="menC"
                     /locus_tag="OO544_001135"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HCR6766783.1"
                     /translation="MRSAQVYRWQIPMDAGMVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQIVLLAWVNNWLAGDGELPQMPSVAFGVSCALAELADTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSPLPLVDADVLEQLL"
     gene            complement(25965..26822)
                     /gene="menB"
                     /locus_tag="OO544_001136"
     CDS             complement(25965..26822)
                     /gene="menB"
                     /locus_tag="OO544_001136"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HCR6766784.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNVGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(26837..27595)
                     /gene="menH"
                     /locus_tag="OO544_001137"
     CDS             complement(26837..27595)
                     /gene="menH"
                     /locus_tag="OO544_001137"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HCR6766785.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VYLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILNFWLVGYSLGGRVAMMAACQGMPG
                     LCGVVVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(27592..29262)
                     /gene="menD"
                     /locus_tag="OO544_001138"
     CDS             complement(27592..29262)
                     /gene="menD"
                     /locus_tag="OO544_001138"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HCR6766786.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     SGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLILIVVNNNGGQIFSLLPTPKSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETALADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(29351..30646)
                     /gene="menF"
                     /locus_tag="OO544_001139"
     CDS             complement(29351..30646)
                     /gene="menF"
                     /locus_tag="OO544_001139"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HCR6766787.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIKAREFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADNVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(30725..31030)
                     /gene="elaB"
                     /locus_tag="OO544_001140"
     CDS             complement(30725..31030)
                     /gene="elaB"
                     /locus_tag="OO544_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HCR6766788.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(31085..31546)
                     /locus_tag="OO544_001141"
                     /pseudo
     CDS             complement(31085..31546)
                     /locus_tag="OO544_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311182.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            31611..32528
                     /gene="rbn"
                     /locus_tag="OO544_001142"
     CDS             31611..32528
                     /gene="rbn"
                     /locus_tag="OO544_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HCR6766789.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPMEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
BASE COUNT         7897 a         8803 c         8139 g         7691 t
ORIGIN      
        1 cgttgttggc taaagaaaag ccggtagaag gcagtccact gtttttacta caaatgtgca
       61 tggaacaatt agtggcgatt ggaaaaattt gatatgagac aggatggcgc atgaacgcca
      121 tcctgcagta gcattactct ttattaagga attttactgc cttatcaggg aaatcagtaa
      181 acagcccatt tacacccgct ttgttataca gagcatcata taactgattc acatcagtag
      241 tgtattcagg cagtttatcc gaccgcacgg tataaggatg cactaccagt ttattctgct
      301 gagcatcttg caccatgcca gtgagtttga tattacccgg ctgcgatgtt tcctcaatca
      361 acatatggta atccggcccg ataccgtcgg catattccgc gacttgcttc atggcacctg
      421 gcttaaacat ccagtcatag ctgtaattaa cccagcttcc gtccggctgt ttctgctgcg
      481 tttcattcca gtcggtatag gcaatcagct gtaccagatt gagatccatg cccattttag
      541 gttccagctc attcttaata cgcttcagct catcagcatc aaaacattgc aaataaactt
      601 tatcgtcctt accggtgtaa ccatatttct tcagcacttc cagcgttttt gccgcaatat
      661 ccttcccttc ctgatgatgg aaccacggcg ctttgatttc tggatagata ccgatatttt
      721 tcccggtaga gtgatttaac ccctgaacaa attcaatctc ttcttcaaag gtgtgccccc
      781 ggaagtcgga cttacccatt gggaaacgcc ccggataagt ctgtactttt ttaccgtttt
      841 caatatcgaa accttcggta aatttcagcg acttaatttc atccagcgtg aaatctatcg
      901 cgtagtaacg accgtctttg cgcgcccgat ccgggaaacg atcggcaaca tcagtaacac
      961 gatcgagata atggtcatgc agaacaacca gatggtcgtc tttggtcatc accaaatcct
     1021 gttccagata atccgctccc tgcgcatacg ccatcgcttt tgctggcagc gtatgctccg
     1081 gcaaatatcc actggcaccg cgatgggcga tgactatttt ttcgttgctg tccgccgcca
     1141 ttgcactgct tcccatgact atagtgctca tcatgatcgc catgctaagg tttttcagct
     1201 tcaatttcat gccattagcc tccgttgcgt ttttgcagta attgttcatg gcgacgtttt
     1261 tcgccaatca tcacaacaat caacaagata accgccagaa tgctgccgcc aatcattacc
     1321 ataaagccgc catcccagcc gaagaagtcc acggtgtagc caacaatcgc gctcgccgcc
     1381 accgaaccgc ccaggtaacc aaacagcccg gtaaagcccg ctgccgtacc tgccgctttt
     1441 ttcggtgcca gttccagcgc atgcagaccg atcagcatca caggaccgta gatcaggaag
     1501 ccgataacaa tcatacaaat catatcgacg gttgggttac ctgccgggtt catccagtaa
     1561 acgatagtcg cgatggtcac cagtgtcata aagaaaacgc cggttgcccc acggttgcca
     1621 cggaagactt tatccgacat ccagccgcac agcagagtgc ccggaatacc tgcatattca
     1681 taaaggaagt aggcccagga ggatttatcc agcgcgaaat gcttaacctc tttcagataa
     1741 gtcggtgacc agtcgaggat gccgtaacgc agcagataaa cgaacacgtt ggcgatggcg
     1801 atataccaca gcagtttgtt cggcagtacg tactgcatga agatttgctt cgccgtcagc
     1861 tcctgttccg ctttttcgtt atagtcgtcc ggataatcat ttttgtactc ttcgatcggc
     1921 ggcaagccac aggattgcgg ggtatcgcgc atcatcgcaa aggcgaataa tgccaccaga
     1981 atggcgcaga aagcaggcat atagagcgcc gcatgccagt cattgaacca ggccatcccc
     2041 agcaggaaca gcagcggcgg aataccacca ccgacgttgt gcgcacagtt ccacactgac
     2101 acaatgccgc cacgttcttt ctgcgaccac cagtgcacca tagtacgacc acacggcggc
     2161 caccccatcc cctggaacca accgcagagg aacaacagta caaacatcac cgcaatgctc
     2221 gacgtcgccc atggcacaaa gcccataaac aacatcactg ccgccgccag aatcaaacct
     2281 gcgggcagga aaacgcgcgg attcgagcga tccgataccg aacccatgat gaatttcgaa
     2341 aatccataag caatcgagat ccccgaaagg gcaaaaccta aatcaccgcg tgagaatccc
     2401 tgctcaacca gataaggcat agcaagcgca aagttcttac gaaccaaata gtaagccgca
     2461 tagccaaaga atatccccag gaaaatttgc cagcgcaatc gacgataagt cggatcgatc
     2521 tccgcggcag gtaagcgcgc tttgtgtggc gctggtttaa aaatactcaa cattgatagc
     2581 ctccgtggcc cgtggtctta tttatgatta acagcctgat tcagtgagag aacctgccgt
     2641 ttcttgagtt gccgcgatgt taagaaaaca ttcataaatt aaatgtgaat tgccgcacac
     2701 attattaaat aagatttaca aaatgttcaa aatgacgcat gaaatcacgc ttcactttcg
     2761 aatcatgagc gaatatgcgc gaaatcaaac aattcatgtt tttactatgg ctaaatggta
     2821 aaaaacgaac ttcagaggga taacaatgaa aactcgcgac tcgcaatcaa gtgacgtgat
     2881 tatcattggc ggcggcgcaa cgggagccgg gattgcccgc gactgtgccc tgcgcgggct
     2941 gcgcgtgatt ttggttgagc gccacgacat cgcaaccggt gccaccgggc gtaaccacgg
     3001 cctgctacac agcggtgcgc gctatgcggt aaccgatgcg gaatcggccc gcgaatgcat
     3061 tagtgaaaac cagatcctga aacgcattgc acgtcactgc gttgaaccaa ccaacggcct
     3121 gtttattacc ctgccggaag atgacctctc cttccaggcc acttttattc gcgcctgcga
     3181 agaagcaggg atcagcgcag aagctataga cccgcagcaa gcgcgcatta tcgaacctgc
     3241 cgttaacccg gcattgattg gcgcggtgaa agttccggat ggcaccgttg atccatttcg
     3301 tctgaccgca gcaaacatgc tggatgccaa agaacacggt gccgttatcc ttaccgctca
     3361 tgaagtcacg ggactgattc gtgaaggcgc gacggtgtgc ggtgttcgtg tacgtaacca
     3421 tctcaccggc gaaactcagg cccttcatgc gcctgtcgtg gttaatgccg ccgggatctg
     3481 ggggcaacac attgccgaat atgccgatct gcgcattcgc atgttcccgg cgaaaggatc
     3541 gctgctgatc atggatcacc gcattaacca gcatgtgatc aaccgctgcc gtaaaccttc
     3601 cgacgccgat attctggtgc ctggcgatac catttcgctg attggcacca cctctttacg
     3661 tattgattac aacgagattg acgataatcg agtgacggca gaagaggttg atattctgct
     3721 gcgtgaaggg gaaaaactgg cccccgtgat ggcgaaaacg cgcattttgc gggcctattc
     3781 tggcgtgcgc ccgctggttg ccagcgatga cgacccgagc ggacgtaacg tcagccgtgg
     3841 catcgtgctg ctcgaccatg ctgaacgcga tggtctggac ggatttatca ccatcaccgg
     3901 tggcaaactg atgacctatc ggctgatggc tgaatgggct accgacgccg tgtgccgcaa
     3961 actgggcaac acgcgcccct gtacgaccgc cgatctggca cttcctggtt cacaggaacc
     4021 cgctgaagtt accttgcgta aagtcatctc cctgcctgcc ccgctgcgcg gttctgcggt
     4081 ttatcgtcat ggcgatcgca cgcctgcctg gctgagcgaa ggtcgtctgc accgtagcct
     4141 ggtatgtgag tgcgaagcgg taacagcggg tgaagtgcag tacgcggtag aaaatttaaa
     4201 cgttaatagc ctgctggatt tacgccgtcg tacccgtgtg gggatgggta cctgccaggg
     4261 cgaactctgc gcctgccgcg ctgccggact gctgcaacgt tttaacgtca cgacgtccgc
     4321 gcaatctatc gagcaacttt ccaccttcct taacgaacgc tggaaaggcg tgcaacccat
     4381 cgcctgggga gatgcactgc gcgaaagcga atttacccgc tgggtttatc agggactgtg
     4441 tggtctggag aaggagcaga aagatgcgct ttgatactgt cattatgggc ggcggcctcg
     4501 ccggattact ctgtggcctg caactgcaaa aacacggcct gcgctgtgcc attgtcacgc
     4561 gtggtcaaag cgcgctgcat ttctcatccg gttcgctgga tttgctgagc catctgcctg
     4621 atggtcaacc ggtgacagac attcacagtg gactggaatc tttgcgtcag caggcaccag
     4681 cccatcctta cagtcttctc gaaccacaac gcgtgctcga tctcgcttgc caggcgcagg
     4741 cattgatcgc tgaaagcggt gcgcaattgc agggcagcgt ggaactggcc catcagcgcg
     4801 ttaccccgct cggcactttg cgtgcaacct ggttaagttc gccagaagtt cccgtctggc
     4861 cgctacccgc gaagaagata tgtgtagtgg ggataagcgg gctgatggat ttccaggcgc
     4921 atcttgcggc ggcttcgttg cgcgaactgg atctttcggt tgaaaccgca gaaatagagc
     4981 tgccggaact ggatgtcctg cgcaataacg ccaccgaatt tcgcgcggtg aatatcgccc
     5041 gtttccttga taatgaagaa aactggccgc tgttacttga tgcgcttatt cctgtcgcca
     5101 atacctgcga aatgatcctg atgcccgcct gcttcggcct ggccgatgac aaactgtggc
     5161 gttggttgaa tgaaaaacta ccttgttcac tgatgctttt gccaacgctg ccgccttccg
     5221 tgctgggcat tcgtctgcaa aaccagttac agcgccagtt tgtgcgccag ggtggcgtgt
     5281 ggatgccggg cgatgaagtg aaaaaagtga cctgtaaaaa tggcgtagtg aatgaaatct
     5341 ggacccgcaa tcacgccgat attccgctac gtccacgttt cgcggttctc gccagcggca
     5401 gtttctttag tggcggactg gtagcggaac gtaacggcat tcgcgagccg attctcggcc
     5461 ttgatgtgct acaaaccgcc acgcggggtg aatggtataa gggcgatttt tttgcgccgc
     5521 aaccgtggca gcagttcggt gtaaccactg atgagacgct acgcccgtcg caggcagggc
     5581 aaaccattga gaacctgttt gccatcggtt cggtgctggg cggatttgat cccatcgccc
     5641 aaggatgcgg cggcggtgtt tgtgccgtca gtgctttaca tgccgctcaa cagattgccc
     5701 aacgcgcagg aggccaacaa tgaacgacac cagcttcgaa aactgcatta agtgcaccgt
     5761 ctgcaccacc gcctgcccgg tgagccgggt gaatcccggt tatccagggc caaaacaagc
     5821 cgggccggat ggcgagcgtc tgcgtttgaa agatggcgca ctgtatgacg aggcgctgaa
     5881 atattgcatc aactgcaaac gttgtgaagt cgcctgcccg tccgatgtga agattggcga
     5941 tattatccag cgcgcgcggg caaaatatga caccacgcgc ccgtcgctgc gtaattttgt
     6001 gttgagtcat accgacctga tgggtagcgt ttccacgccg ttcgcaccaa tcgtcaacac
     6061 cgctacctcg ctgaaaccgg tgcggcagct gcttgatgcg gcgttaaaaa tcgatcatcg
     6121 tcgcacgcta ccgaaatact ccttcggcac gttccgtcgc tggtatcgca gcatagcggc
     6181 tcagcaagca caatataaag accaggtcgc tttctttcac ggctgcttcg ttaactacaa
     6241 ccatccgcag ttaggtaaag atttaattaa agtgctcaac gcaatgggta tcggtgtaca
     6301 actgctcagc aaagaaaaat gctgcggcgt accgctaatc gccaacggct ttaccgataa
     6361 agcacgcaaa caggcaatta cgaatgtaga gtcgatccgc gaagctgtgg gagtaaaagg
     6421 cattccggtg attgccacct cctcaacctg tacatttgcc ctgcgcgacg aatacccgga
     6481 agtgctgaat gtcgacaaca aaggcttgcg cgatcatatc gaactggcaa cccgctggct
     6541 gtggcgcaag ctggacgaag gcaaaacgtt accgctgaaa ccgctgccgc tgaaagtggt
     6601 ttatcacact ccgtgccata tggaaaaaat gggctggacg ctctacaccc tggagctgtt
     6661 gcgtaaaatc ccggggcttg agttaacggt gctggattcc cagtgctgcg gtattgcggg
     6721 tacttacggt ttcaaaaaag agaactaccc cacctcacaa gccatcggcg caccactgtt
     6781 ccgccagata gaagaaagcg gcgcagatct ggtggtcacc gactgcgaaa cctgtaaatg
     6841 gcagattgag atgtccacaa gtcttcgctg cgaacatccg attacgctac tggcccaggc
     6901 gctggcttaa actcctttct gatgcccggt aagcatgtgg ttaccgggca tttttgcgta
     6961 cacgattccg tgcccaatgt atgcgttgca acgcagtgaa aattcctctg aaaacgtctc
     7021 gcaaaggctg aaactggcag atgtcaaagg cctgggataa ccgtaatgtc gcgtcatcat
     7081 aaatatcagg tgacggacaa ccatgaccga atcaacaacc tcctccccgc atgatgcggt
     7141 atttaaaacc tttatgttca cacccgaaac cgcacgggat tttctcgaaa tacatttgcc
     7201 agaaccactg cgcaagcttt gcaacctgca aaccttacgc ctggaaccca ctagttttat
     7261 tgaaaaaagt ttacgcgctt actactcgga tgttttgtgg tccgtggaaa ccagcgacgg
     7321 tgacggctat atctactgcg tgattgaaca tcaaagctct gcagaaaaga atatggcttt
     7381 tcggctaatg cgctatgcca ctgccgccat gcagcgtcac ctggataaag gctatgacag
     7441 agttccgctg gtggtgccat tgctgtttta tcatggcgaa acatcgccct acccgtactc
     7501 acttaactgg ctggatgagt ttgacgatcc gcaacttgcc cggcagttgt acaccgaagc
     7561 ttttccgttg gtggatatca ccatcgtacc tgacgatgag atcatgcaac atcggcgtat
     7621 agctctgctt gaactgattc aaaagcatat tcgcgaccgc gatttaatcg gtatggtcga
     7681 caggatcacc acgcttttgg ttaaaggctt cactaatgac agccagctac aaacactgtt
     7741 taattatctg ctgcaatgcg gcgatacctc ccgtttcacc cgttttattg aggagatttc
     7801 cgaacgttca ccactacaaa aggagagatt gatgactatt gctgagcggc tacggcagga
     7861 aggacaccaa attggctggc agaaaggtat gcatgaacaa gccattaaaa ttgctttgcg
     7921 catgctggaa cagggcattg atcgtgacca ggtgctcgcg gccacccagc taagcgaagc
     7981 cgatctggca gcgaataacc actaattaac acaggcccac agccgatccc catgggcctt
     8041 tgatatcaat aactaccttt tatgcgtggc ccatttttgc ctgatttaaa catcgccagt
     8101 cgttgatcca gggcatcgct gtagagcatc gtgtcaacgc caacagcgac aaagttcgct
     8161 ccccacgcca ggcattgctg cgccatatca ggagccacag ccagaaaacc agccgcttta
     8221 cccgcagcac ggatccgccg aatgctggtt tcaataattc gctgcacttc cgggtgcccg
     8281 gcgttatccg ggtagcccaa cgacgcagaa agatccgctg gtccaataaa cacgccatca
     8341 atcccttcga cgtcgaggat ctcgtccagg ttatccagtg ccgttttact ttccacctgc
     8401 actaacagac aaagcgaatc gttgacttgc gccatgtaat tctcaatgcg tccccagcgc
     8461 gcagcccgtg ccacactggc cccaacacca cgctcaccgt agggaggata gcgcgtggca
     8521 gacgccacct gacgagcctg atcggcagta tcgaccatcg ggatcaatag agtttgcgcg
     8581 ccaatatcca gaacttgttt aatcagcgat ttactgcctt ccaccggacg gatcacgggt
     8641 tggctggcat agggcgctac cgcctgtagc tggtgataaa gatcctgaat ggtgtttggc
     8701 gcgtgctccc cgtcaatcag caaccagtca taaccggaag tagcggcaat ttctgccata
     8761 taagaagtag tcgagcttaa ccacagacca atttgcactt cgcccttgcg taaacgttct
     8821 ttaaagggat tggttaataa tgcgttcatc gtgttcctta acctatttaa tgatgtgcca
     8881 cgtcggtctg cgcaacagta cgatttacac gcagagtgaa aataatcagc gaaccgatga
     8941 ccgccaccgc tgccagtgtc agtaatcctg ccgcatcgct ggcaaataac gtttctgctt
     9001 tcacgcgcag gatcggggca ataaacccgc ccactgcacc aaacaggttt acaaaaccaa
     9061 tccccgcagc cagcgccgta ccggaaagca gttgtgtcgg catcgtccag aacaccggct
     9121 gcacggcaat aaacccgata gctgcaacac acagcgctac gatcgccatt actggagaaa
     9181 gcagcccgga cagaccaata ccaatgcccg ccgccagtaa tgtcagcgct gcgacattac
     9241 gccgttcgcc ggttttatcg gaatagcgcg gaataagcca ggtcccaaac aaggccgcaa
     9301 cccacggaat ggcggtgacc accgacgctg taaagcccac ttttgttccc agcaatgccg
     9361 caacctgggt cggcaggaag aaaattaatc cgtaaaccgc cacctgaatg gtcaggtaga
     9421 taatcgctag ttgccagact cggccattac gcagcgcatc gctcagccga gaagtcactt
     9481 tctgttgttc ttcacttgcc agttgattga taagcaacgt tttttcttgt ttactcagaa
     9541 aacgtgcctg ctccggtgtg tcatcaagcc aaaagaatgt gaatacccca gcaccgactg
     9601 ccaacaatcc ttcaatcaca aacatccaga accagccggg atgccccata aatccatgca
     9661 tctccaacag cgcgccggaa agcggtgatc ccagtgttaa cgccagcggt gcgcccatat
     9721 agaacagccc cataatgctg gcgcgattac gctgcggaaa ccattgcgag gtgagataaa
     9781 tcataccagg gaaaaatccg gcctccgcag caccaagcag agtgcgaact atcagaaatt
     9841 tcgcttcagt atcggcccat gccatggctg ccgaaagaaa tccccacagc agtgttgtcg
     9901 taccaatcca ggttctggcc cccagtttgc gcatcaaaag attcgccgga acacccagaa
     9961 acgcatatac cacaaagaaa atgcctgctc ccagcgcata agcttcatta ctcaacccgg
    10021 tatcaatctg gtaggtctgt ttggcaaaac cgatattcga acggtcgaga aacgccagca
    10081 catacagcgc caacataaac ggaattaaac gcacacggtt tttcttcacc acggcgtcaa
    10141 gcaaagcggt gctcataaaa agctccttag aatatgtggg cgttaccgtt gtcacagcaa
    10201 cgcccggagt gattagtggc tgtaggggcg tttcaaattg cagtcacgat tgagttcgac
    10261 gccaaaaccg ggtttatcaa gcactgattt atgaatacga ccattcaccg gaaccggctc
    10321 attgagcaga atcgggtcaa actgcggacg catcgttgaa caatccgggc tggtcatcag
    10381 gaattcgctg aatggcgtat tggtgaaggt gatcaccgca tggtgagagt aaacagacga
    10441 accgtgcggc accaccagtt gccccctgga tttggcgatt gcggcaattt ccaccagcgt
    10501 ggttaaacca ccgcaccagc caacatccgg ctgcataatg tcgataccgg tttctgaaag
    10561 cgtacgaaaa gattgcagtg tgccgtggtg ctcaccgctg gtgaccatca ttccgactgg
    10621 cgcgttgcgt ttcagttcgc gataaccttc atactgctgt ggcggcaggc actcttcgat
    10681 ccatttcagg ttatagggcg cgcaagcgtg ggccagtttg gtggcatagt tcacgtcctg
    10741 actcatccag cagtcgagca ttaaccagaa atcctcaccg catttttcac gcatatccgc
    10801 gaccataacg gcatctttgc ggatccccgc atcgccatca tgtggccccc agtgcgtcgg
    10861 cattttgcca ccgataaagc ccatctcttt tgccagatcc ggacgcgcgc ctgtggcgta
    10921 gaactgaatc tcatcacgaa ctgcgccgcc taaaagttta taaaccggaa gcccgaccac
    10981 tttgccgaac agatcccaca gagccagatc gacacaggaa atcgtgttca tcaccaggcc
    11041 accagagccg gagtaataca gggtggcatt gagcatttga tcatggatca gtttgatatc
    11101 actgacacat ttaccctcaa tgaaacggtt aagatgtttt tcgacaataa aacagcccat
    11161 ttcaccggct gtcgaaacag cgaatccggt ctggccgttt tctgcttcga cttcaacaat
    11221 caacgtgcca agaacgttaa tgccaaatga ctggcgtgac tgctcgtaat cgcggtattt
    11281 actcatcggg gtggcaatat gatcgtcaat ccagtgattc gccccctggt cgtgataatc
    11341 accgcgccag cacctttttc tgctgtcgca ccgccagtaa accaggcgcg aacctgtttg
    11401 atttttggta gggtcatgat gttctccatt gttatgaggc ctgtaagtca aagggacttt
    11461 tccatcccaa cagacgtgaa atatccctgg cgcaggcaat ggccttgccc gccagataat
    11521 cacggtattc ttcattgatt tgtaagcggg taccgaccac cgagatcgca gcggtaagct
    11581 cgttattggc gttaaacacc ggcgcagcga cacaacggac atcggggtaa tcttcgccgt
    11641 tgtcatagct ccagccctga cggcgaatac gcgccagttc ttcgtgaagt tgctgtggat
    11701 gagtaatcgt tgtgggtgtc gcctgctccc agaccagccc ttcgataata ctttgctgta
    11761 ccgctgcagg ttgccaggca agcaggcatt tacctattcc ggagcgataa agcgaaaggc
    11821 tttttccttc atgggaacgc acgctgatag tggctgatga ctccactttt aaaatgtaat
    11881 aagcgctgcc gttatcaata atccccaggt gacataacag cccggtggta tccatcagtt
    11941 gcgtcaaacg cggtcgagcc agttcccgga gatccatctt gcttaacgca tgaccagaaa
    12001 gctccaccag cctggtccac aaacaaaaat tttcctggtg atcaaggcta agaaaacgct
    12061 gacgcctgag ttcattaagc agcaaatagg ccgtgctttt agggattccc aatgtgtcaa
    12121 tgatcgtcgc agcactacag gggccaatgc gggcaatcaa attgagaata tcaatagccc
    12181 gtgtcagggc gggaactttg cttgattcca acatactgga ctccagtctt agatactgga
    12241 atcagtctcg ttgctcaggg ggagtaaaat tgtgaagcgc atcaaatccc gctcctcgtc
    12301 cagcggactg gagaaattac gcacaaattt gtgaatcagt gcacggattt tttcttattg
    12361 agtacaacag gttggacaag ttttgccgga gggggaatgt gccggtgcag taaaccggca
    12421 cagagtggcg gaatcagaca gataaggtca tggactcaac aacctcaatc cagccatgct
    12481 cactggcgat atcctggccg tttaaccaac ggcgcagcat attcagtgcc atcattgcgc
    12541 acacttcctg acggatagcc aggctgtagc gtgtagtgct gaaacgcaca cgcagagcga
    12601 aagtgccgtc tggcgtggct agcgcaaagt tgagatgctc gttctcgaaa cccgaaacag
    12661 ccagtgccag cccggcaaaa tggttggccc gccgttctgt aatccagtgc gcagtttgcg
    12721 ccagggtttc ttcctgtgaa ggaaccactt cacacgccag caatggagca cctgcgcgag
    12781 aaagttgcaa agccaataaa ccaccggtga attgctcgct caacgtcagg ctgaactggc
    12841 ggttttgcaa ttcgcgactg atctgcgcgg gcagtccttc ggtgccttcg aaaatcacgc
    12901 tctgtccggc aacacgttta acgtccagcc acagtttttc catcgcctgt tgctcgcttg
    12961 ccggtccggt gagtttcagt tcgatgatcg gcattgagga gcgatagccc attgttacgc
    13021 ccggcggcag ttgtagagtg tccaggcttt gtgccagatc gctttccgaa cgaccaaagg
    13081 tagttaaacg cagacaaacc ggcgactgtg gtaaagaaaa acgctcgcgc aggcgcggca
    13141 ggatttcgtg ctcgaccatc accttaaatt ctgacggtac gccgggagta aagaacatca
    13201 ggcaacgatt aagccgcacg gcaaaaccac aggcggtgcc taccgggtta ttgataaatt
    13261 cagcactggc aggcagctcc gcttgtttac ggttgctcgg tgccattact cgtccacgtt
    13321 cgtggaaata gcgttccatc tctttgagcc aggcttcatg cagcaccagg ccttcacctt
    13381 ttgctgtcgc agcggcgagt gcacttaaat catcactggt cggtcccagc ccgccgttaa
    13441 cgatcagcac atcggcgtgc tgactacgtt cgcgaagaat ggtgactaag tcatcaaggt
    13501 tatcccccac cgtattgcgg cgagataatg gcaacccctg atgaaagaaa aaatcggcca
    13561 gccaggcagc gttagtgtca acgatttgcc cgtgtaacac ttcatccccg gtggataaca
    13621 tttccacttt taacatggtt atctcctgct tttgttatcg aatcactata acgcaaaggg
    13681 agataaggcg tagtaagaaa ctgaatggcg cgacagaatg ccgcgccgga agggatcaga
    13741 aactggcgtt aaccccaaca tacgggccgt cagccacggc gttgtcgcgg ttaccgtctt
    13801 tacccgacag attcaggtag cgataacccg cctcaatact gactggatgc ataatggtgt
    13861 aacgcgcgcc agcattcgct tcctcataac ttttaatacc gctggagagt gaatccggag
    13921 agtagtaata ctcgccaaac aaacggaagc tgttgccaat tttccactgc aaaccacctc
    13981 ctaccgctgc ggcataacct tcatcgccgt aattcgggtt ggtgtaaacg ccttttccgc
    14041 caacggtcgc cattaacggc ccgagaggca gattcaaccc cagccccacg ccagcgacgt
    14101 caccgtcgtc gtcgttatgt gtccagttac cgcttaacgc caggcccgtc gattcagtcc
    14161 caaaaccgac accaatattg gtgtattctt tacccgcctg accgctgatg ctcattgcat
    14221 tgaccgatgc agaaaccaac agcattccgg ccagacctgc tagcgcaatt tttttcatta
    14281 tcccctttcc cacaagcaat taatcaaaat tcccaaagct gcgggattgt actgcctggc
    14341 acagaggatt caatgagcga aaaaagggat ctagtcactt attgtaacta aatacttctt
    14401 aaataattaa acgccgaaaa aataaacaca ctttaccgat acacacaaaa ataataataa
    14461 gtgcagttat catcgcaaaa gagattactg tcactttcac atatttctga gttaccgtat
    14521 tctcatggtt tctttttatt tataaggaat acccctgtgc gacaacggac tattgtatgc
    14581 cctttgattc aaaatgatgg tgcttatttg ctgtgcaaaa tggccgacga tcgcggcgtt
    14641 ttccccggtc aatgggcgct ttcgggtggc ggcgtggagt ctggcgaacg aattgaagag
    14701 gcactacgcc gcgaaattcg cgaagaactg ggagaacagc tgcttttgac agaaatcacg
    14761 ccgtggacct tcagcgatga tattcgcacc aagacgtatg cagatggtcg caaggaagag
    14821 atttatatga tttacctgat ttttgactgc gtttctgcca accgagaagt gaaaataaac
    14881 gaagagtttc aggactacgc gtgggtaaaa cctgaagatc tggtgcatta tgatttaaat
    14941 gttgccaccc gaaaaacgtt acgtttgaaa ggtcttcttt aacaatgatg acagccatca
    15001 ctggctgtca gattgaaaat ttagtggtta acgaattccc catccagata aactttgcct
    15061 ttttccacat gcatgactaa accatccaga taatcaggtt taaatgtcgc atcacgctta
    15121 tttttagcaa tatatgtcag gcaatgatta tgggtgaaaa taacgatatt tttatcaggc
    15181 gctttgcttt gtaagtcctt aattgcacta taaatctcat taccgcactg aagaagtcgt
    15241 ttatctaccg tcagtttttt acccgcggaa aaccaggtcg ccgactgaat ggtccggacg
    15301 gtattactgg aataaagatc gaaatcaggg atgtcagcgc taaaagcgtt gcccagttca
    15361 cgggcatcct gggtaccttt aaccgtaata cctgttttat ctgacaagca ttgattggtt
    15421 gaacgatcgc aacgttcagc atggcgaaac aaaacgacaa ccgggtgctg ctgtgccagt
    15481 ctggccaatg ttttattgtc gatacgtggc aaaccattgc tactccaggc ggcatgagta
    15541 cccagtccgg caattgcagc gagcgccagt aaaatgatga aatatttttt tgacttcaat
    15601 gatgagcggc aaaaagctaa cacgtaaact ccacctatag acaagcgcaa ccagacaatt
    15661 accgtgaaat tgagcgacat ttctggcgat aattagcagt tggtgtaata ttaaaaatcc
    15721 tacgatgtcg gcaaaatgcc tcaaaatttt gccaaatgca aagcctaaat aagaaaaaat
    15781 ataaaaattt caatatttac gtctaatatt agtttcttaa ggttaagtta atattctatc
    15841 cttaaaattt cgctccaaat ggcaaaatat acacaacact ctttatagca aatataagtg
    15901 gacaggtatt caatggcgga aggaaaagca atgtcagaat ttttgccttt ttcgcgacca
    15961 gcaatgggcg tggaggaact cgctgcagtt aaagaggttc tcgaatccgg ttggatcaca
    16021 accggtccga agaatcaggc gcttgagcaa gccttttgcc agttgacggg aaatcagcat
    16081 gccatcgcgg tcagttcagc caccgccgga atgcatatca cgctaatggc gttggaaatt
    16141 ggcaagggcg atgaagtgat tacgccttcc ctgacctggg tttcaaccct caatatgatt
    16201 tccttgttgg gtgcaacgcc ggtaatggtg gatgtcgacc gcgatacgct gatggtcacg
    16261 cctgaagcga tcgagtcagc cattacgcca cgcactaaag ccatcattcc ggtgcattat
    16321 gccggtgcgc cagcagatat tgacgccatt cgcgccattg gcgaacgtta cggcatcgca
    16381 gttatcgaag atgctgccca tgccgtcggt acgtattaca aagggcgaca tattggcgca
    16441 aaaggtaccg ctattttttc atttcatgcc attaaaaata ttacctgtgc ggaaggtggc
    16501 ctgattgtaa ctgataatga aaaccttgcc cgccagctac ggatgctgaa atttcacggt
    16561 ctgggtgtcg atgcctatga cagacaaacc tggggccgtg caccgcaggc tgaagtctta
    16621 acaccgggct ataagtacaa tctgaccgat attaacgccg cgattgccct gacacagtta
    16681 gcaaaattag agcacctcaa cacccatcgg cgcgaaattg cccagcaata tcagcaagca
    16741 ctggcagctc tcccctttca gccattaagc cttcccgcct ggccgcacgt tcacgcctgg
    16801 catctgttta ttattcgtgt cgatgaacaa cgttgtggta tcagtcgcga tgcgttgatg
    16861 gaagcgttaa aagaaagagg cattggtacc gggttacatt tccgcgccgc tcacacacaa
    16921 aaatattatc gcgagcgttt tcccacgttg tcgttaccga ataccgaatg gaatagagaa
    16981 cgcatctgtt ctttgccgct gttcccggat atgactaccg ccgatgccga ccgtgtcatc
    17041 acagcccttc agcaactcgc aggacaataa gccatgtttg aaatccaccc tgttaagaaa
    17101 gtctcggtgg ttattcccgt ttataacgag caggaaagct taccggaatt aatcaggcgc
    17161 accaccacag cctgtgaatc gttggggaaa gagtatgaga tcctgctgat tgatgacggc
    17221 agtagcgata attccgcgca tatgctggtc gaagcctcac aagcggagaa cagccatatt
    17281 gtgtctattt tgcttaaccg caattacggg caacattcag cgattatggc gggattcagt
    17341 cacgttactg gcgacttaat tattaccctt gatgccgatc tccagaatcc gccagaagaa
    17401 atcccccgcc tggtggcaaa agccgatgaa ggttacgacg tggtagggac tgtacgccag
    17461 aaccgccagg acagctggtt tcgtaaaaca gcttcgaaga tgattaacag gcttattcag
    17521 cgcaccaccg gcaaagcgat gggtgactac ggttgtatgc tgcgcgccta tcgccgtcat
    17581 attgtcgatg cgatgttgca ctgccatgaa cgtagcacct ttatcccgat tctggcgaat
    17641 atcttcgccc gccgtgccat tgaaattcca gtacatcatg ccgagcgtga gtttggtgaa
    17701 tccaaataca gttttatgcg cctgattaat ttgatgtacg acctggtgac ctgccttacc
    17761 acaacgccgc tacgtatgct gagtctgctc ggcagcatta ttgcgattgg aggttttagc
    17821 attgcggtgc tgctggtgat tttacgcctg accttcggac cacaatgggc ggcagaaggc
    17881 gtctttatgc tatttgccgt gctgtttact tttattggcg ctcagtttat cggcatggga
    17941 ttgctcggtg aatatatcgg caggatctac accgatgtcc gcgcccgccc ccgctatttt
    18001 gttcagcaag ttatccgtcc atccagcaag gaaaatgaat aatgaaaacc gtcgtttttg
    18061 cctaccacga tatgggatgc ctcggtattg aagctctgct ggctgccggt tacgaaatta
    18121 gcgccatttt tacccatacc gataatcccg ctgaaaaagc cttttatggt tcggtggctc
    18181 gtctggcggc ggaaagaggc attccagttt atgcgccgga taacgttaat catccgctgt
    18241 gggtggaacg cattgcccaa ctctcaccag aggtgatttt ctctttttat tatcgccatc
    18301 ttatttgcga cgagattttg cagctcgctc ccgcaggtgc atttaatctg cacggttcgc
    18361 tgttaccaaa atatcgtggt cgcgcgccgc tgaactgggt gctggtgaac ggtgaaacgg
    18421 aaacgggcgt tacattgcac cgaatggtaa aacgtgccga tgctggggcc attgtggcgc
    18481 aactgcgcgt tgccattgcg ccagacgata tcgctattac gctgcatcat aaattgtgcc
    18541 atgccgcgcg ccagctactg gaacagacat tacccgccat taaacacggt aatattctgg
    18601 aaattgccca gcgcgaaaac gaagccacct gttttggtca cagaacgcca gatgacagtt
    18661 tccttgaatg gcataaaccg gcatccgtac tgcacaacat ggtacgtgcc gttgccgatc
    18721 cgtggccggg tgccttcagc tatgttggca atcagaaatt caccgtctgg tcgtcgcgtg
    18781 ttcatcctca tgccagcaaa gcacagccgg ggagcgtgat ttctgttgcg ccactgctga
    18841 ttgcctgtgg cgatggcgcg ctggaaatcg tcaccggaca ggcgggcgac ggcattacta
    18901 tgcagggctc gcaattagcg cagatgctgg gcctggtgca aggttcacgc ttgaatagcc
    18961 agcctgcctg caccgcccga cgccgtaccc gggtactcat cctcggggtg aatggcttta
    19021 ttggcaacca tctgacagaa cgcctgctgc gcgaagatca ttatgaagtt tacggtctgg
    19081 atattggcag cgatgcgata agccgttttc tgaatcatcc gcattttcac tttgtcgaag
    19141 gcgatatcag tattcattcc gaatggattg agtatcacgt caaaaaatgt gatgtcgtct
    19201 tgccgctggt ggcgatagcc acgccgattg aatatacccg caacccgctg cgcgtatttg
    19261 aactcgattt tgaagagaat ctgcgcatta tccgctactg cgttaagtac cgtaagcgaa
    19321 tcatcttccc gtcaacttca gaagtttatg ggatgtgtag cgataaatac ttcgatgagg
    19381 accattctaa tttaatcgtc ggcccggtga ataaaccacg ctggatttat tcggtgtcaa
    19441 aacaattact tgatcgggtg atctgggcct atggcgaaaa agagggttta cagttcaccc
    19501 tcttccgccc gtttaactgg atgggaccac gactggataa ccttaatgca gcgcgaattg
    19561 gcagctcccg cgctattacg caactcattc tcaatctggt agaaggttca ccaattaagc
    19621 taattgatgg cggaaaacaa aaacgctgct ttactgatat ccgggacggt atcgaggcgt
    19681 tgtaccgcat tatcgaaaat gcgggaaatc gctgcgacgg cgaaattatc aacattggca
    19741 atcctgagaa cgaagcgagc attgaggaac tgggcgagat gctgctggcg agcttcgaaa
    19801 aacatccgct gcgccatcac ttcccaccgt ttgcgggctt tcgtgttgtc gaaagtagca
    19861 gctactacgg caaaggatat caggacgtag agcatcgtaa accgagcatc cgcaatgccc
    19921 gccgctgcct ggactgggaa ccgaaaattg atatgcagga aaccatcgac gaaacgctgg
    19981 atttcttcct gcgcaccgtt gatcttacgg ataaaccatc atgaccaaag tcggcttacg
    20041 cattgatgtc gatacctttc gtggcacccg tgaaggcgtg ccacgtctgc tggaaatctt
    20101 gagtaagcat aatattcagg ccagcatttt tttcagcgtc ggcccggaca atatgggccg
    20161 ccatctctgg cgactggtga agccacagtt tttgtggaag atgctgcgct caaacgcggc
    20221 atcgctttat ggctgggata ttttactggc aggtacggcc tggccaggta aagagattgg
    20281 tcatgccaat gccgatatca ttcgtgaagc ggctaaacat cacgaagtcg gcctgcacgc
    20341 ctgggatcac catgcctggc aagcccgtag cggtaactgg gatcggcaaa caatgatcga
    20401 cgatattgca cgcggtcttc gcactctgga agagattatc ggtcaaccgg taacctgttc
    20461 tgccgctgcg ggctggcgtg ccgaccagaa ggtgatcgaa gcaaaagaag cgttccattt
    20521 gcgctacaac agcgattgtc gtggggccat gccgttccgt ccactgctcg aatcaggaaa
    20581 ccctggcact gcgcaaattc cggtgacctt acccacctgg gatgaagtga ttggtcggga
    20641 tgtgaaagca gaagatttta acggttggtt actcaaccgc atcctgcgag ataaaggcac
    20701 gccggtttat accattcatg cagaagttga aggctgcgct tatcagcata attttgtgga
    20761 tctcctcaaa cgcgcagctc aggaaggcgt gacattttgc cctttaagcg aactgttatc
    20821 agagacgttg ccgctcggac aagttgttcg cggaaatatt gccggacgtg aaggctggct
    20881 gggttgccaa caaattgcgg gtagtcgctg atgaaatcgg tacgttacct tatcggcctc
    20941 ttcgcgttta ttgcctgcta ttacctgtta ccgatcagca cgcgtctgct ctggcaaccc
    21001 gatgaaacgc gttatgcgga aatcagtcga gaaatgctgg catccggcga ctggattgtg
    21061 ccccatctgt tagggctacg ttatttcgaa aaacccattg ccggatactg gattaacagc
    21121 attgggcaat ggctatttgg cgcgaataac tttggtgtgc gggcaggcgt tatctttgcg
    21181 accctgttaa ctgccgcgct ggtgacctgg tttactctgc gcttatggcg cgataaacgt
    21241 ctggctctac tcgccacagt aatttatctc tcattgttta ttgtctatgc catcggcact
    21301 tatgccgtgc tcgatccgtt tattgcattc tggctggtgg cgggaatgtg cagcttctgg
    21361 ctggcaatgc aggcacagac gtggaaaggc aaaagcgcag gatttttact gctgggaatc
    21421 acctgcggca tgggggtgat gaccaaaggt tttctcgccc ttgccgtgcc ggtattaagc
    21481 gtgctgccat gggtagcgac gcaaaaacgc tggaaagatc tctttattta cggctggctg
    21541 gcggttatca gttgcgtact gacggttctc ccctggggac tggcgatagc gcagcgggag
    21601 cctgacttct ggcactattt tttctgggtt gagcatattc aacgctttgc actggatgat
    21661 gcccaacata gagctccgtt ctggtactac gtgccggtca tcattgccgg tagcctgccg
    21721 tggctgggat tactccccgg tgcactgtac acaggctgga aaaaccgcaa gcattccgca
    21781 accgtctatt tgttgagctg gacgataatg ccgctgctgt ttttctccgt cgctaaaggt
    21841 aaattgccca cctatattct ttcctgcttt gcatctctgg caatgctgat ggcgcattac
    21901 gctttgctgg cagcaaaaaa taatcctctg gcgctgcgga ttaatggctg gattaacatc
    21961 gcttttggct tcactggcat tattgccaca tttgtggtct ccccgtgggg accaatgaac
    22021 acgccggtgt ggcaaacctt cgagagctat aaagtctttt gtgcctggtc gattttttcg
    22081 ctatgggcat ttttcggctg gtacacctta acaaacgtcg aaaagacctg gtcttttgcc
    22141 gcgctttgcc cgctggggct ggcgttgctg gtaggatttt caattcctga cagagtcatg
    22201 gaaggaaaac atccgcaatt ttttgtcgag atgacacaag aatcacttca gccaagccgc
    22261 tatattctta ctgatagcgt cggtgttgcc gcaggtctgg catggagcct gcaacgcgat
    22321 gacatcatca tgtatcgcca gacaggtgag ttgaaatacg gccttaatta tccggatgcg
    22381 aaagggagat ttgtcagcgg tgatgagttc gcaaactggc ttaatcaaca tcgtcaggag
    22441 gggattatta cactcgtgct ttcggttgac cgcgatgaag atatcaacag tctcgccatt
    22501 ccgcccgcag atgccatcga tcgtcaggag cgtctggtgc tgattcagta tcgtcccaaa
    22561 tgatctggct aacattagtc tttgccagct tgcttagcgt tgccgggcag ttgtgtcaga
    22621 aacaggcaac ctgctttgtg gcgataaaca aacggcgcaa acatatcgtg ctgtggctgg
    22681 gactggcgct ggcttgtctt ggtcttgcca tggtgctctg gctgctggtc ttgcagaacg
    22741 taccggtagg cattgcctac ccgatgttaa gtctgaattt tgtctgggtg acgctggctg
    22801 cagtaaaact gtggcacgaa ccggtatcgc cgcgtcactg gtgtggggtg gcgttcatta
    22861 ttggcggcat tgtgatcctc gggagtacgg tgtaatgggc ctgatgtggg gattattcag
    22921 cgtcattatt gcctcagttg cgcaattaag cctgggtttt gcggcgagtc atctgccgcc
    22981 gatgacgcac ttatgggatt ttatcgcggc tctgttagct tttggcctgg atgcccggat
    23041 actgttactc gggctgctgg gttatctgct ttccgttttt tgttggtata aaacgctaca
    23101 taaacttgcg ctaagcaaag cctatgcctt gctcagtatg agttatgtgc tggtatggat
    23161 tgcttcaatg gtacttcccg gctgggaagg gactttttcg ctgaaagcac tactgggagt
    23221 agcctgtatt atgagcgggt tgatgctgat tttcctgccc acgacaaaac aacgttactg
    23281 agttttccct gccactttac actgccgttc ccactcatcg gggctataac tgcttgccga
    23341 gaggactttc accgtatgca gcttttcacg attaatacaa taaagcgcat tctgcaaagg
    23401 cgagagtaaa tcccccactt tcacggcgaa gtcactcttc acttcggcga tcatttttat
    23461 cgcgccgcca gcatcgcaga gcataagtac atgtctgtta tcttgtttat tgcagcacga
    23521 ttttttgacc agccattcca ttgcattatc ctgtttgcta agagttttca catcaattta
    23581 cagattaaca gataaaccta ttatcaacgg caatggaatg gcaaaattgc gaagtgaaac
    23641 taatgatttc gtaatcctgg cgttgcaccc actcttttaa cgcctgacgt gaaattttaa
    23701 taccgccgtt tttcagctcc ggcggtaaag ttagccagcg caccggttgt tgaaaacgag
    23761 ccagcttatc tttcacccat tcgccaagat caacggtttg ctggtcatac tccaccaccg
    23821 ctaccggtcg atgaccaaac tccttgtcgg caacggggac gataaacacc tgcaaaaccg
    23881 caggatgtgc agcaattacg cgctcgactt cttccggctg aataccctct ccgccactga
    23941 agaacagatt gtctaaacgt ccgacaatgg tcagcttgcc attatgcatc tcaccgcgat
    24001 cgcgcgtagc gtaccagcct tcgtcattaa ccagtgaaac cagttgcccg ttacgccagt
    24061 aaccttctgc catactggca gcccgcagcc acacttcatt attaacgatt ttcacttccc
    24121 gacccggcag cggcgaacca acgtctgcca ggccgtcggc ttctttcgca cacaccgtgg
    24181 aggcaaactc ggtcagacca tagccgcaaa agcaacgaat cccctgctcg cgcgcctgtt
    24241 ccgtcaactc gaccgggata gccgcgccgc caagtaacac cgctttcagg gaaacggaac
    24301 tgcggttaac cagcaaacgc cagagttgtg ttggcaccag tgaagcgtga gtacagcctg
    24361 ccagcatttg ctccaatggc tgtttatcac gcaccgtcat ccgcgcacca gcgtataacc
    24421 agcgccacat aattccctga ccggagacgt gaaacagcgg taaagagagc aaccagtcat
    24481 cgtgatcgcc aaacggaatc agcgataaca caccttgcgc actggcaaga tgggcctgat
    24541 aagtatggac agcggctttc ggcaaaccgg tagaacccga ggtcaacgtc attgagcaca
    24601 gacgcgtcgg ctgccacgta gcggcatgtg cgccttcaac ccgctgaatg tgcagcgacg
    24661 ttaatgccgg aaacgtgttt tccccatccg gcaccagcgc aaattgcagc gtcagattgg
    24721 gcagcaattc ttcaagcaac ggttgcggca gctgagggtt cacgggcaac acccgcgccc
    24781 cgcattgcag taacgccagc caggcgagca gcgtttgcgg cgtattccac gcccgcaaca
    24841 tcacgccgct gccctcaacc acaccctgcg ccgcaaagcc cgtagctaac tcatcgacgc
    24901 gagcacaaag ctcgcgccag ttgagttgct cgtcattaag acgtaaggcg atggcttctc
    24961 cccgcacttg ccgccagtga cgccacggcc agtcagagaa gatcataaca actgctccag
    25021 tacatcagcg tccactaacg gcagtgggct ccccggccag cgacgaacct gctgcgcctg
    25081 catcagatcc agcgtgtcca gtcccggaat ggtgtctggc gttaaccagg cagcaatccg
    25141 cgccagttgc gttagaccta agctcgactc aatggaagaa ctgatcaccg ccgtcagccc
    25201 cagcgcatgc gccgcctgta cctgctcgcg tactttttcc agactgcccg tgagcgtggg
    25261 tttgataact accgcgcgca cgccctcttc agccacaaag gcaaaatccg gctcgcgcag
    25321 gctttcatcc caggcaatgg caatgccggt ttcacgggca aacgctcgcg aatcatcgcg
    25381 ggttttgcac ggctcttcga gaaacgcgat gcggtggcga taatccgggt taacatattt
    25441 ggcaaactgc tgacctttca gcggtgtcca ggcgcgattt gcgtcaagac gcaaatgcag
    25501 atccggaatt gcctccagca acagattaac caccatgccg tcgcgcacgg cttcgtacaa
    25561 tcccactttg accttcgcca ctttctcgcc tggcatatca gcaagtttga ggatcagatc
    25621 gtcaggatcg ccattacaca gtggtgccgc acggtaattg gccgcctgcg ggagcgtatc
    25681 tgccagctcc gccaatgcac agcttacgcc aaaggcaacg gaaggcatct gcggtagctc
    25741 gccatcgcct gccagccagt tatttaccca agcaagcagc acaatttgcg cctcttccca
    25801 ggtttcctga ctgaagcccg gcagtgggga gatctccccc cacccttcgc gctcgccttc
    25861 acgcaggcaa acatacagcc cgtcgcgggt ttttaaccgc ctgtcgcgca gaaccatccc
    25921 cgcgtccatg gggatctgcc agcggtatac ctgcgcgcta cgcattacgg attccgttta
    25981 aatttgctga aatcaggctg acgtttctgg ttgaaagcgt tgcgaccttc ctgaccttct
    26041 tccgtcatgt agaacagcat ggtggcgttg cccgccagct cctgcagacc cgcctgaccg
    26101 tcacagtcgg cgttcagcgc agctttcagg cagcgcagcg ccatcgggct gttttgcagc
    26161 atttcgcggc accaacggac ggtttctttt tccagatccg ccagcggtac cacggtgttg
    26221 acaaggccca tatccagcgc ctgttttgca tcgtactgac ggcacaggaa ccagatttca
    26281 cgcgcttttt tctgtccgac gatgcgagcc atgtaggaag cgccccagcc gccgtcgaag
    26341 gaaccgactt tcgggccagt ctgaccgaag atggcattat ctgccgcgat agtcaggtcg
    26401 cacatcatgt gcagaacgtg accaccgccg atggagtagc cagccaccat cgcgacaacc
    26461 ggtttcggac aggtacggat ctgacgctgg aagtccagca cgttcaggtg atgtacgccg
    26521 gaatcatctt tatagccgcc gtaatcacca cgcactttct ggtcaccacc ggagcagaac
    26581 gctttatcgc ctgcgccagt cagaatgatc acgccgacgt tgtcgtcata acgcgcatct
    26641 gccagtgcct ggatcatctc ttttaccgtc agaggacgga aggcattgcg cacctgcgga
    26701 cgattaatgg tgatttttgc gataccgtcg gtggattttt cataacgaat gtcctcaaaa
    26761 ccttcggagc agtcgtgcca ttcaaccggt gcgtaaagca ttgcttcatc aggataaatc
    26821 atattgtgtc ctttagtcag aaacgcaaga tctgcgccag acttgcgata acgccagcgg
    26881 gattttcccg atgcgcgtta tgtccggcgc gaggaatgac atggcagtcg gcagccagtt
    26941 ccgccgccaa ggcgcggaat ttgctgtcac gttcaccaca taaataataa aacgcaaatg
    27001 tgcgggcgct aaggttagca cgtaaatcag gctggacggc gagagaagtc gcctccagca
    27061 tggcggcaag ggttgcgcca ttattgttac tgcgcagcgc caccagctcc cggcgttgat
    27121 cgtcattgag cgaggcaaaa acaggctgtt gataccagtc ggcaaatacc gccgttaacg
    27181 gttctgtgcg aaaacgctgc gcccattggc gatcggaacg ctgacgttcc gcgcgttgtt
    27241 cagcattttg caacccagga tgcccgcctt cgacaacaac cccacaaagc cccggcatcc
    27301 cctggcaagc cgccatcatc gccacccgtc ccccaagcga gtaccccacc aaccagaagt
    27361 taaggatgtt gtaactaacc agggttttac gcagtaagtc ggtgacatca tcaaatccat
    27421 cgacgctaat cgccgccgaa ccaccgtgac ctgggagata aacatacaac cgtgagtagt
    27481 cggcaaacgc ctcgcccact tcttgccatt cgtggcaatc gccggaaaaa ccgtgcaaaa
    27541 acaccagcca gggtaaacct ggtttgccgt gttttgcctg cgcgtgcagg atcataaatg
    27601 gcttacctgc gccagaagtt gctggagcgt ttgcgcacca tcggtgtcgt taaccaccat
    27661 ttcaattacc gtggtggttg gcgtgcgcca ggcatcggca agtgccgttt caagttcctg
    27721 ccagttttgc ggacgatgat atttcagctc gaacatcgcg gcggcgtgct caaaatggac
    27781 gttttgcggc atcagataga aacgctcgcg ctcgcttttc ggcgttggca gcagcgagaa
    27841 aatttgcccg ccgttgttgt tcaccacaat taatatcagc ggcgcggaaa cctgacgcaa
    27901 taacgccagc gcgttgagat cgtaaagtgc ggagagatcg cccacaatcg ccagcgtcgg
    27961 tttgccgctt gcccgctgaa cgccggcggc agtcgagagc agcccgtcga taccgctggc
    28021 cccacggttg ctgtacaccg ggtaacctgc cggaagttgc gaaagcgcat caatcagacg
    28081 taccaccagg ctgttaccga caaacaattg cccctgttca ggcagatagt cgctgatgcg
    28141 atgcgccagt tgcgcttcgc caaacgcatc gcgacgggca ataaccgcct gcattgcctg
    28201 ttccgccagg cgcgggattt caacgcacca gggctggcgt ttttctgccg gatgcagctc
    28261 cagccagtcg gcaatattgg caattaagcg gcgtccacga tggtgcgccg gatcaagccg
    28321 cccttcgatg tcatccacaa tccagtactc ttcgggttca cagcttgcct gccattgcag
    28381 gagccgtttg cccgtcaggc tgcttcccag ttgcaccaca atttgcgcct gttgcagttc
    28441 gctggtcgct ttggcattgc ctaaccagag atcggcacac ggcagcggct gtccggtttg
    28501 cgacagcacg tcgccaatca gcggccagcc aagagtttgc gcccacagtg caactttttt
    28561 gccctcttcc gcactcatgc gcccggcaac caccacgccg cgtttttgtc gccagaagaa
    28621 ccagtcgcgc tgtttttcac tttccagacg aggcgcttca cgcagccacg gtttgtcgtc
    28681 ctgccaccag tcgcccagcc gctgttgcca gctaagcccg gtgtcgtcca tttcgccata
    28741 cagcggttcg gcaaacgggc agttgatatg gactccacca gaatgaagcg taccgagagc
    28801 atggtcgatg gtagaaacca gccaacgtgc ggggatatcc tgggtcgggc gcggcaatga
    28861 aatactgtgc gtggggtgag aggcgaacat tcccggctga cgaatcgcct gattcgcgcc
    28921 gcagtcaatc agctccggcg ggcgatcggc ggttaacagg atcagttttt ctccggttaa
    28981 cccagcttca atcagtgccg gatagagatt tgctaccgcc gtaccggagg tcacaatcac
    29041 cgccaccggc tgcttgctga ctttcgccag ccccagcgcc agatgcccca acccacgctc
    29101 atcgaaatgg gtgtggtgaa tgaatgcgga attctccgcc gccgctaacg ttaacggtgt
    29161 agaacgcgag ccaggggcga tacagatgtg tctgacgccg tgacgcgtta atgcttccag
    29221 aatgaccgcc gcccagcgtc ggttaaatgc gcttactgac atgagtttgt ccggtatcaa
    29281 tattgcggct aagtataagg agctacaaaa atagattatt gatatgaatc ggtaatgatg
    29341 cgactcatta ctattccatc tgtaataaag tacgcagccc tgccgctttg ttgtcgattt
    29401 cctgccactc ttgctcgggg tcggaaccac ggacaatgcc cgcgccagca tataatcgca
    29461 cgacattgcc gctaattttt gctgagcgca gggaaacgca gaattcgctt tgctgtaatg
    29521 agagatagcc cgcagaaccg gcgtaccatt ctcgggtgaa cggttcgtga cgggcgataa
    29581 actgtcgcgc cagatcgcgc ggtaagccag caactgctgc cgttggctgc aactgatgta
    29641 aacagatcac attatccgct ttgttgagtg aagtccagat acagcggcga agatgctgca
    29701 ctttacgcag acgcagtacc tgcggcggta aaacatccag cgtctgggta tcggcctgta
    29761 accgttggca gatatcttcc accaccagca tattctcgcg ctggttttta tcatccgcca
    29821 tcagccactc acctaactgc tgcgcctgct tatcatcagg gtgatttgct accgttcccg
    29881 ccagcgcttc agtacgcagc gctttgtcac gccgccgcca taaccgttcc ggtgaagagc
    29941 caagaaaagc attttcgcca tcaaaggcca tgtaaaaatg gtagcaattc agattcagtc
    30001 gacgactggc agccatcatc gccgccgcgt tgaccggact tgcgaaatgc aggtcagttg
    30061 cccgagcgag caccactttg tcgagctcac cttcggcgat ggttttcgtt gccagttcga
    30121 ttaattgcgt ccagcccgtt ttgtccggcc agtgttgttc tcgcgtggtg gttaaatgta
    30181 acccaggcaa gggcttgata ctcaccagtg tggcgataaa ttctcttgcc ttaatcgcat
    30241 cgtgctgaag ggagctttcg ctgaatagcg tcagccgcag cgtggcttta ccgccacagc
    30301 gtcgccattc caggcgtggt aaaagtaaat tgccctgcga cgggtcaaac gcattcagcc
    30361 cccaaatgcg taagtcggcg tgttccgggt gctggcgaag aaaacgttgt gcctggtcca
    30421 acgacgtaaa acgggtaatc gcgcccagga cggcagcttc ttcatcacca ttacgttgtt
    30481 gccagtagaa ttgcgggtat gtttgctgac tggccagcca gctcaaggca tcaaaagcgt
    30541 ctttgagagg gaaaggaata tcgataaccc gaatgccggg tgtcgccgga atctcttgcg
    30601 acaaatggcg cagtagattt tccagcgccg tagtaagtga ttgcacgcgg acctctccct
    30661 gttaaaagcc tcacattata cggggtacta caaaaaaaat gcagtacccc ggtgtaggga
    30721 ggttttaacg gcgtgccagc aacagtccta gtaccagccc aacggccgcg cccacaccaa
    30781 ttccttgcca gggtttttcg tggacgtagt catcagcacg ataaactgcc tgcttcgccc
    30841 gatagtaata actgtctgaa gcctggctaa cccgtttttt cacatcatcc agcgcttttt
    30901 ctgcacgcgc tttcagctca acatatttct gatcggcggg atcgccagag gagcggagca
    30961 cctcttccag tgtttcacta agcagcgtca ggtcgtcatc gatacgtgta tcaccaaact
    31021 gattagacat tctcgttctc catttgcgta aaacctgtca ctaactatag acaagggttg
    31081 ttgattacgc ctgaattacc tcgcgtgcca tcccaatgtg tgggatacca tcttcttcat
    31141 agacctccgt cacagggata aaaccgaaac tctggtagaa gttttgcaga tgcgcctgcg
    31201 ccccgaggta aacaggctta tcaggccagt gatgcgtgca ggtgtcgagg gttttactca
    31261 tcagttgctg ccccactttt tcgccacgca acgcttcact gacaatcacc cgaccaataa
    31321 cgaccggctc aagatcatca tcacttttca gaatcctcgc atatgccacc agttcatcat
    31381 ttttccaacc gagaatatgg cgattatccc ccgtcaaatc atcaccatcg atatcctgat
    31441 aagggcaatt ctgctcgaca acaaacaccg cgcaacgcag ttgtaataag gcataaagct
    31501 gggaaaccga taattcagag tgatgcagat cttaccattc aatcataatg ctctcctttt
    31561 agatgtatgc cttatactag ccggaatatt ttttgaaacg tgatgaactc atggaattaa
    31621 tttttttagg tacttcagcc ggtgtgccaa cccgcacgcg caatgtcacg gcaatattgc
    31681 ttaacctgca acatccgacc cagagcggac tttggttgtt tgactgcggt gaaggcaccc
    31741 agcatcagct actgcatacc gcctttaacc ctgggaaact ggacaagatt tttatcagtc
    31801 accttcatgg cgatcatctc tttggtttac ccggcttgct gtgcagtcgt tctatgtcag
    31861 gcattatcca acccttaacg atttatggtc cgcaaggtat ccgtgaattt gtggaaaccg
    31921 cgctgcggat tagcggctca tggaccgatt atccgctgga aattgtcgaa attggcgctg
    31981 gcgaaattct cgatgatggc ctgcgcaaag tgaccgctta tccgatggaa cacccactgg
    32041 aatgttatgg ctatcgtatt gaagaacatg ataaaccggg tgcattaaat gcccaggcat
    32101 taaaagctgc tggcgtgccg cctggcccac tgtttcagga attaaaagcg ggcaaaacaa
    32161 tcacgctgga agatggaagg cagattaacg gcgcagatta cttagctgct ccagtgccag
    32221 gtaaagcgct cgctattttc ggcgataccg gcccctgcga tgccgcactt gacctggcta
    32281 aaggtgtcga tgtcatggtg cacgaagcga cgctggatat aaccatggaa gccaaagcca
    32341 atagtcgcgg ccatagctct acacgccagg ctgcgacact agcccgtgag gctggagtcg
    32401 gcaagctaat cattacccac gtcagctcgc gctatgatga caaaggttgt caacacctgt
    32461 tacgtgaatg caggtcaatt ttcccggcga ctgaactggc gaatgatttc accgtgttta
    32521 acgtttaact
//
LOCUS       DAJTZL010000028        31358 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.028, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000028 DAJTZL010000000
VERSION     DAJTZL010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31358
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.028"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..2328
                     /gene="torZ"
                     /locus_tag="OO544_001143"
     CDS             <1..2328
                     /gene="torZ"
                     /locus_tag="OO544_001143"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HCR6766790.1"
                     /translation="DGKILTAERWGAMNVEVKDGKIVSSTGALAKTIPNSLQSTAADQ
                     VHTTTRIQHPMVRKSYLDNPLQPTKGRGEDTYVQVSWEQALKLIHEQHDRIRKANGPS
                     AIFAGSYGWRSSGVLHKAQTLLQRYMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVY
                     EQQTSWPLILENSQVVVLWGMNPLNTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPI
                     RSETIDFFGDNATWIAPNMGTDVALMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYL
                     RGKSDNTPKSAAWAAEITGVPEAQIVKLAELMAANRTMLMAGWGIQRQQYGEQKHWML
                     VTLAAMLGQIGTPGGGFGFSYHYSNGGNPTRVGGVLPEMSAAIAGQASEAADDGGMRA
                     IPVARIVDALENPGGKYQHNGKEQTYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEM
                     IVVSECYWTAAAKHADIVLPITTSFERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDF
                     DVFADLAELLKPGGKEIYTEGKDEMAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKL
                     IEMRRSEKNEQYVRYGDFRADPVKNALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAP
                     DEWKGTADEKQLQLLTAHPAHRLHSQLNYAELRKKYAVADREPITIHTEDAARFSIAN
                     GDLVRVWNKRGQILTGAVVTDGIKKGVVCVHEGAWPDLENGLCKNGSANVLTADIPSS
                     QLANACAGNSALVYIEKYTGNAPKLTAFDKPAVQA"
     gene            complement(2493..3464)
                     /gene="cmoB"
                     /locus_tag="OO544_001144"
     CDS             complement(2493..3464)
                     /gene="cmoB"
                     /locus_tag="OO544_001144"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HCR6766791.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(3461..4204)
                     /gene="cmoA"
                     /locus_tag="OO544_001145"
     CDS             complement(3461..4204)
                     /gene="cmoA"
                     /locus_tag="OO544_001145"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HCR6766792.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCSRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            complement(4245..4640)
                     /locus_tag="OO544_001146"
     CDS             complement(4245..4640)
                     /locus_tag="OO544_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HCR6766793.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            complement(4693..4932)
                     /locus_tag="OO544_001147"
                     /pseudo
     CDS             complement(4693..4932)
                     /locus_tag="OO544_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310605.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5182..5712)
                     /locus_tag="OO544_001148"
     CDS             complement(5182..5712)
                     /locus_tag="OO544_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6766794.1"
                     /translation="MSQGKEPVIERIGMLTKRYPSRSAAARAWGININTLNSYFKGGQ
                     TQPAPRESILIRISESEGVSLEWLKEGKGELPNDIGIYGNFGCSGDKGDQLTQMLSFL
                     TKDEREQLTNLLARKGVETVLTLLNKENQDLLQLEGRKRLAALMIVDMPDDCVREILE
                     KTETSRQSNPVESKAG"
     gene            5903..6151
                     /locus_tag="OO544_001149"
     CDS             5903..6151
                     /locus_tag="OO544_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6766795.1"
                     /translation="MSRREVSDHDWPPEIIKARLHMAGLSLRSLSLKAGYSRDSLKSV
                     LRTPCRPYQQIIADALGVSPEEIWPSRYQVKSYMRKAS"
     gene            6153..8243
                     /locus_tag="OO544_001150"
     CDS             6153..8243
                     /locus_tag="OO544_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6766796.1"
                     /translation="MYVIAKELIGAPGMPATTKGIRQALQRYVQGKSCCSRRRSGSKA
                     TEYSIDCLPEVTQQALRERYALQLMTQKADESPAPVVTKARRSPAVVDAVEAYRGSPQ
                     LMVERLNALTENQRKVAEARIAIVSEVLKIAQQPGFSCAKAIRFLVNSLARSQLDERI
                     VAMVETANAKKGNSRALSEITLKRWIAAFNKAQNAAERLLLLAPGKRQEIKAEDINWL
                     PEFLAQYRQSNGRPMTEAYEDFVAEWQHRHADEPYMLDIMPSYDTIRRAMKKLPEVVK
                     QKGRVTGSEYRQLEGFTRRDWSRMPVNYVWIGDGHGMKLKCAHPVHGRPFAPEVTFVI
                     DGGTRFVVGWSLDLAENVFAVAGAIQHGIRHHGKPFLYYSDNGSGETADVLDKEIVGI
                     LPRLGINHPTGIAGNPQGRGIIERLNRTLPMRIARKYRTYFGKGADRETLRKTNRDLR
                     SAFSALQQGKRLNARQQSAMRDLPSWSELIDAIRDGVEWYNNRPHDELPMKPNGKHYS
                     PAEFRKKRLAEEDTEIEWLSDVELRDMFRPMVERPVRRCEIRWLNNIYYAPELRDEHG
                     RKVLISYDIHDAERITVRRPDGSVICEAVWDGNKREAFPVSAEYYKQQQRLKGMRKRA
                     EEKIRDAEDEVVNVLEHKPQEPWLENIYRPVGNTVTVQQPVADDEPDEEYERNFQRGL
                     QLLEAKLKESDPLA"
     gene            8326..9258
                     /locus_tag="OO544_001151"
     CDS             8326..9258
                     /locus_tag="OO544_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR6766797.1"
                     /translation="MTDINDVIKTIDELIDGGVLTQYAIAREAGISDGTLSAFRKGKY
                     KGDNAAVAASLRSWYENWNKQSALPEPPQFVETQTVQELRALFQAVRLMGCINVIVGV
                     PGVGKTATARNYCQEQPNTWMITLSPAHSSVTECLLELADALGIDYTRANKGALSRAI
                     RRRLMGTRGLVIVDEADHLGIDGLEQLRAIQDATGIGMVLIGNPRGLFKGGRRAFDDL
                     SRLFSRLARTKQLRKAKKADVLAIAMAWGISGEAELAVMQAIAEKPGALRVLTHTLNQ
                     AWLTASGEGAALTEKHINAAFKEVYTNPELLSQV"
     gene            9261..9482
                     /locus_tag="OO544_001152"
     CDS             9261..9482
                     /locus_tag="OO544_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6766798.1"
                     /translation="MAVFNIPDIYGRFYLVNFDNVKVISLAENKECGDLLFEFNDRTR
                     MVISAGLDREGATEVYSGICRSVGAKQVS"
     gene            9495..9749
                     /locus_tag="OO544_001153"
     CDS             9495..9749
                     /locus_tag="OO544_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6766799.1"
                     /translation="MNMQSCGNKMNLFDSLNSARRLTELAGAVLERSKRYPQRFALKT
                     TPPVGNVQGTGEIEITIQTNGLRRRVKATRISGCTVYWEV"
     gene            9751..10032
                     /locus_tag="OO544_001154"
     CDS             9751..10032
                     /locus_tag="OO544_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host nuclease inhibitor protein"
                     /protein_id="HCR6766800.1"
                     /translation="MKKNLIAWAWSSGLIEFGYVLPEGALPIVAGKPATVRHVIEVMA
                     RHGRDEQEQLLVPGIPEAVTEEEAFNAMIRFCREVRRRVSYPNRTRTRG"
     gene            10029..10301
                     /locus_tag="OO544_001155"
     CDS             10029..10301
                     /locus_tag="OO544_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6766801.1"
                     /translation="MSKVVRIIFEYKEDVICKNSDGSTHRGVSLDIRSTGIKKKDNGP
                     AMIFGVVMLAESRNFAEFVAMKASAFMKDRGMASGVINGNEFNQQG"
     gene            10306..10599
                     /locus_tag="OO544_001156"
     CDS             10306..10599
                     /locus_tag="OO544_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6766802.1"
                     /translation="MSKVRVIFEFNHVSHDEKLAGNDCVEVHEKIGVDVKTERDTDNR
                     PTSLCDVYASILQYHSPAIIQFLSAEFQASAQSFGADAIIKRHRVHKASGTLQ"
     gene            10611..11141
                     /locus_tag="OO544_001157"
     CDS             10611..11141
                     /locus_tag="OO544_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HCR6766803.1"
                     /translation="MAKRVTKLKAAAEAAPQTREEVSRDIRTLGDIQREALRLETAMN
                     DEVAEITARYTPQIENLKKQIKVLFKGIQDWCKTNRDELTNGGKTKTANLTTGTVSWR
                     LGTPSCSVSRDVEGVIEMLRRMGLERFIRTKEEVNKEAVLAEPDAVKGIAGIKVNKGA
                     ESFYVEPFEQDAGLNK"
     gene            11287..11820
                     /locus_tag="OO544_001158"
     CDS             11287..11820
                     /locus_tag="OO544_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6766804.1"
                     /translation="MIDAKVLEGVKNWLCIYGRLTCGVLAEKMNMPPSSMVYFLRDAV
                     DAGVLTECNGFYDIPRPRPVQPVRRKCSQESAADDVQWCGFRKSLPWIEGHDIPSMAW
                     EFAQGVLTCETVYVVAEVDEQAMKEGVPQFVMAYIDIRLGVIICGLSGWNITEHVLRY
                     LIVDRTAAPAGISAEVA"
     gene            11820..12335
                     /locus_tag="OO544_001159"
     CDS             11820..12335
                     /locus_tag="OO544_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6766805.1"
                     /translation="MFFKTSNPSALAAWQKYQQDCQKVKDEAKRLEAVLNVACRSVFV
                     SGISGFYFKGLRFTEDKYPFHRDLWRKPTASNGWSCTPRTSRIPKTLRVASDELNSLW
                     REYSPVTYARTDALLFWLGIDFSAILFGPVKWFCVDDVIYLQCGVKPAKQKMTEILSD
                     EFYAAEKRVRG"
     gene            12339..12890
                     /locus_tag="OO544_001160"
     CDS             12339..12890
                     /locus_tag="OO544_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SANT/Myb domain-containing protein"
                     /protein_id="HCR6766806.1"
                     /translation="MMKLQPMGLKGRAPAHVRPWTPEEDALLIALYSSTPVKDIAARI
                     KRTVWAVYNRTGVLRSSYPELLKYKHPRFTPDEDKFIRKNARTMTCQQMGEYLGRNKD
                     SVRCRAGMIGAGLTKCGELRPGTHISDDDVRLIRALRDSDYPRRLSFREIGEKFGISE
                     HSAHAVYYRRRTAEDAVLRELTP"
     gene            12887..13216
                     /locus_tag="OO544_001161"
     CDS             12887..13216
                     /locus_tag="OO544_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6766807.1"
                     /translation="MITTLFVESDEPLVCAAGMPLCGGTLTGVYFGDLRGYPWHSLND
                     AFPPDMEAAVLIVQYGHRQELRIGHMGYEGFFVDEETGACLEDEDGQVTHWCHISALP
                     ELREVNA"
     gene            13213..13563
                     /locus_tag="OO544_001162"
     CDS             13213..13563
                     /locus_tag="OO544_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6766808.1"
                     /translation="MRSYIDNEKLETISDCLSLLAKIKETIEEIKFQLEYAPCGDDAW
                     RNAARKALAVFQKQRRAVEYRLAVLRQEEKERNIRRHERVNDFLVRELKERVPESVFF
                     ECEAIARSKALETD"
     gene            13579..13887
                     /locus_tag="OO544_001163"
     CDS             13579..13887
                     /locus_tag="OO544_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6766809.1"
                     /translation="MSIELRSSYEYRKILIAGGMKPEDAEKFVSFMDKECDKRDMPEI
                     IMDDMILDSAVALSPLWIVHTLAEIAKRSDKQAAVAALRLLAELSCSVNPNTFFDEPG
                     "
     gene            complement(13849..14259)
                     /locus_tag="OO544_001164"
     CDS             complement(13849..14259)
                     /locus_tag="OO544_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000687318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2513 domain-containing protein"
                     /protein_id="HCR6766810.1"
                     /translation="MKIDYDEVLSILTTFQDAETPFLTLQDLGMAEAEGEEKDKKVFH
                     LMLLADNGSIVNGDMQSETPKYIGFFFHSLGVGFRNTPIMLTQQGHDLANALRKKPIL
                     ERIKKEFTDAPFDLIKEVTKSMLTRFVKERIGID"
     gene            14324..14836
                     /locus_tag="OO544_001165"
     CDS             14324..14836
                     /locus_tag="OO544_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp16 family protein"
                     /protein_id="HCR6766811.1"
                     /translation="MNRASLITLIHVAKRDLQLDHETYTSVLLAATGKPSCRDMSPGE
                     LSRVLDVFKKRGFKVRQKPVNRALKPGTVTAKIRAIWKVMHRQGFISDGGETALNRWV
                     KSQTAAQNDGEGVANWQWLEQQPALASDVLERLKRWHRRKMLAALDMSERAVMSYERV
                     CRLYEKSFPR"
     gene            14907..15329
                     /locus_tag="OO544_001166"
     CDS             14907..15329
                     /locus_tag="OO544_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR6766812.1"
                     /translation="MAETQMSMFGDSEQLHALIDRLDDIPDDELKKNWPGTLRDLVDV
                     ISAELHRQGIEPAQAGMLARKVAAAQAGYMGGRGYYLPVGESLFTELRNNEIFSRWSQ
                     GEKIETLRRHYQMSETQIYSVIRNQRRLHQERTQPRLF"
     gene            15401..15901
                     /locus_tag="OO544_001167"
     CDS             15401..15901
                     /locus_tag="OO544_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HCR6766813.1"
                     /translation="MPKLPAPLRKKLIALVLAGAGTFTIATHYTGYWEGKENTTYIDP
                     TGTPTICYGHTGPDVKPGMTKTDEECLELLEKDMKWAFAAIDRHVQVPLTRGQTVALA
                     SWIFWAGETNFRNSTLLRLINAGQMPASCKQYIRWIYSKGVKLPGLEARRSADEWLCR
                     YDLPKV"
     gene            15936..16364
                     /locus_tag="OO544_001168"
     CDS             15936..16364
                     /locus_tag="OO544_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001388992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6766814.1"
                     /translation="MCRCAAAGCAVSAVNNPLPALIAGGCMAALGVMSVLAAVVWGMH
                     QKTQRLEDNNHVLVRERDEARLVLANQQRTLQLISQISKAATNEKQQNMQHSEEQQSI
                     VRRSLATVPAAAVPVPDDVADRVRRAVCEIRAGEAGADPR"
     gene            16348..16566
                     /locus_tag="OO544_001169"
     CDS             16348..16566
                     /locus_tag="OO544_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6766815.1"
                     /translation="MPIPAEWLADCLVPPAPQPFTFGASVTYNLQLLAVIKNCNVDKA
                     SIRRLETQRQHEFTDMAGTTAVPAGKTK"
     gene            16577..16804
                     /locus_tag="OO544_001170"
     CDS             16577..16804
                     /locus_tag="OO544_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HCR6766816.1"
                     /translation="MDDSDCAQAVMERATERALCDRLTRKRRVAETPGRRVCADCGGD
                     IPAARLVAVPDAIRCVNCQNIMEAGHVGQHR"
     gene            16785..17096
                     /locus_tag="OO544_001171"
     CDS             16785..17096
                     /locus_tag="OO544_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000264993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2730 family protein"
                     /protein_id="HCR6766817.1"
                     /translation="MWVSIVKDYVVPILSATATAGGIFMALMRKTFVPREAFEKLSDR
                     VERVESRISGLPTQQEVNRLNIEITTLRGELKATNATLKSVSHQNELLLEQAVRKKHN
                     E"
     gene            17089..17379
                     /locus_tag="OO544_001172"
     CDS             17089..17379
                     /locus_tag="OO544_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HCR6766818.1"
                     /translation="MSDFITEDQRLVILRSLADYNGELGESVLQDCLDDYGHRVSRDT
                     VHTHIAWLAEQGLVRKRTLVNGYFIAELTGRGQDVAEGRATVPGVKKPRARG"
     gene            17382..17963
                     /locus_tag="OO544_001173"
     CDS             17382..17963
                     /locus_tag="OO544_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3486 family protein"
                     /protein_id="HCR6766819.1"
                     /translation="MDKPARGRVRKVDLLPDSIRKPLLEMLREKRLTQVQIREEINRL
                     IREAGLPEEQQLSPAAISREASRNELIARNLRDLREQTKAMMAELGDKPTGETTALIL
                     EMSRALMYRRLRAATESLNSDSDVDMRLIKDILLSAQRAESAAERSIKREKEIRAAFA
                     EEMANAVTDELRGVDGMSEQIEFRIRQMLLGKA"
     gene            17974..18222
                     /locus_tag="OO544_001174"
     CDS             17974..18222
                     /locus_tag="OO544_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6766820.1"
                     /translation="MATDNRSLMSSAHQKAFSLATQLPPETIKVCRELTELSIAGEIS
                     LEQLHSLLAEAATDELSGVDVMTEELESRIKSILTGKA"
     gene            18222..19886
                     /locus_tag="OO544_001175"
     CDS             18222..19886
                     /locus_tag="OO544_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6766821.1"
                     /translation="MNKARETSPSLMQMTPPRKIDLSGEKALLGVDVPDSLDLPGDMP
                     VFLDYQARWFADESQVCIAEKSRRTGLTWAEAGRNVITAAKPKRRGGRNVFYVGSKQE
                     MALEYISACALFSRAFNQLADADVYEQTFWDRDKKEEILTYMIRFPNSGFKIQALSSR
                     PSNLRGLQGDVVIDEAAFHESLDELLKAAMALTMWGARVRIISTHNGVDNLFNQYIQE
                     AREGRKDYSVHRITLDDAIADGLYRRICYVTDQEWSPESEQKWRDDLYKNAPTREDAD
                     EEYGCIPKKSGGAYIPHALIEMAMIRDIPILTFEAPDNFISRAAWLRESEVLTWCEEH
                     LKPLLEKLNPRSRFSFGEDFARTGDLSCFVLLEITESLAKREVFRVELRNLPYAQQEQ
                     VMMYILTRVPALVGAAFDATGNGGYLAEAALLAFGPDIIDCVMLSPKWYGEWMPKLKA
                     EFEDQNILVARHQTTLDDLRHVKVVNGIPQIDKGRTKDQNATAANARRHGDFAVALCM
                     ANRASYMEGFILDESACQALQERSRAMEGGYRDDDEAYHEFDRGGW"
     gene            19886..21475
                     /locus_tag="OO544_001176"
     CDS             19886..21475
                     /locus_tag="OO544_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF935 domain-containing protein"
                     /protein_id="HCR6766822.1"
                     /translation="MGRIIDTDGKPFSFDPEMQSAALDIPQIASRYIEHPASGITPNR
                     AAQCLRGAERGDLIAQSDLAADIEEKDTHLFAELGKRRLAIQGVPWSIEPPPNASANE
                     KKDAEMLDEYLHSADWFDAMLFDATDAILKGYSCMEIEHGMLGKMHIIRAIRWRDSGH
                     FCLNPDDLSELRLRDGSHSGVAFQPFGWIVHQSRSRTGYGGATGLVRTLIWPFIFKNY
                     SVRDLAEFLEVYGLPMKVGKYPSGATPEQKSALMRAVMDIGRRTGGIIPAGMSLEFQA
                     AANGQADPFETMISWGERSISKAILGGTLTTEAGDKGARSLGEVHNEVRREIRDSDLR
                     QLAATLNRDLVYPLYALNTTHTIDIRRLPRICFQTKEPGDITKITSAVMQLSTGMDIS
                     DPWIREQTGIPQPTPGEAIFRVRQSGNEPAQTDRETPPEKQEKTEQTALSARLPEAKS
                     TPRDELDDMGDAVPARRLQEAIDPVLEPVIDAIRTRGLADALADLPALYREMDDSRLM
                     TLLSDAMFAAEMKGMLDDTGD"
     gene            21459..22790
                     /locus_tag="OO544_001177"
     CDS             21459..22790
                     /locus_tag="OO544_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HCR6766823.1"
                     /translation="MTQGIDLGYAATLPSKEAVAYFRVKGAHISWNWFETAADVHARS
                     FTAAKAARLDVVTTLQAEVQRAIDEGISQKAFIRTLTPRLQKLGWWGKQIVVDSDGNA
                     EEVQLGSPRRLALIYNVNTRVAYNAGRYTQMMNNTDTHPFWQYVAVMDSRTRPSHSAL
                     NGLVFRYDDPFWKTHYPPNGWNCRCRVRPLSQARLDAMGLSVSSGEDHLSTRNVEAGV
                     DKQTGEVREMPVTTYSDGTRTMTPDVGWSYNPGSAAFGTDQALIRKLIEVKSPALREM
                     VVQEMNNSPERQLAFRIWAKNIMQTRRGGNDIRTLGFMTESIAQAVESRTGTPPARLL
                     AMSGKNVLHADSVKHQNDGIALMPEDFGRLPAMLAKPKAVLWDKRHKNLMYIVENKDG
                     SVQIAVNAPYSLKRQPDKLDVIVNAYRVINMDKLKSDIRGGMLEVLEGQID"
     gene            complement(22792..22871)
                     /locus_tag="OO544_001178"
     tRNA            complement(22792..22871)
                     /locus_tag="OO544_001178"
                     /product="tRNA-OTHER"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
     gene            22912..23385
                     /locus_tag="OO544_001179"
     CDS             22912..23385
                     /locus_tag="OO544_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HCR6766824.1"
                     /translation="MSSIDAAVVVDVTRLQRVFARLQFVGGGKDLARSVAASLLSSSE
                     MAFEQEKEPDGERWHDWSDPYRKWRTRKGYTPGKILTLNGDLARRLTTDYGDTWALIG
                     SNEPYAAIHQWGGLPGMPPGPAAIGARPYMGFDQVAEQEIMDEIRKRFKKATETP"
     gene            23563..24687
                     /locus_tag="OO544_001180"
     CDS             23563..24687
                     /locus_tag="OO544_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease"
                     /protein_id="HCR6766825.1"
                     /translation="MKTKNTPKLAYAILNAISLSADGDGDWCQIMPAGRVKARDGRPE
                     KPAEGWLINHAAVERMVSRVVALNQPVKIDYNHQTLIKGHPAPAAGFVMASPENFRFS
                     EERGFEVRPDWNPPAIEHLRNNEFPWFSPVIGYDEITGEPVELRMLAITGDPGLTGMN
                     PVAALSADDLYNALNPPLKDTSMNEQLRQLLTALGLTVADGDEFTPELGTAALSALTG
                     IKTRADAHDTLKTQVASLSAELETAKGTPTGGTIDLTKYVPVETYNALRTEYVALSAQ
                     HGSTTLEQLLDKAESEGRIFKSERGYMEGLGQQIGVAALSAQLDARQPVAALTTLQTD
                     TVTVPDKKTATAALSAEDIAAAHLLGKTEAEFLKMKEEMQ"
     gene            24687..25634
                     /locus_tag="OO544_001181"
     CDS             24687..25634
                     /locus_tag="OO544_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu-like prophage major head subunit gpT family
                     protein"
                     /protein_id="HCR6766826.1"
                     /translation="MPTPITPAMITALMTGYRSDFQAGMSMAPSQYQKIAMTVPSTSK
                     SNTYGWLGQFPQFREWIGSRVIEKMKAYGYAIVNKTFEGTVAISRDDFEDDNLGIYSP
                     LFQEMGRAAAAQPDELVFAALRDGLNAACYDGQNFFDTEHPVYPKVDGSGDAQMVSNM
                     FVAKTGSVGAQADYSGPAWYLLDCSRAVKPLIYQDRRKAELVAQTKVDEGRAFTDNEF
                     VFGASARRNVGYGFWQMAYMMQSPLTLDALWHGWSAMRDFKADGGRKLGIKPTHIVVP
                     TSLEKQAVQLLERELFADGNTTVSNEMKGKLELVVADYL"
     gene            25678..26031
                     /locus_tag="OO544_001182"
     CDS             25678..26031
                     /locus_tag="OO544_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6766827.1"
                     /translation="MNEHHTVAATDDSGLQVSGDSPVTVLAEVRCSRAQFRRAGFLFM
                     RGRQQVDVTPEQLARLEAEPCLTVRILQTPGDDAGAVAGVVHAETGADLAEAGQDAPR
                     KKTGNNARQAKKARA"
     gene            26028..26447
                     /locus_tag="OO544_001183"
     CDS             26028..26447
                     /locus_tag="OO544_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp436 family protein"
                     /protein_id="HCR6766828.1"
                     /translation="MNYATETDMRARYREDLLRPLLAVPRSDEPDARKLNRALTDASA
                     LIDSYLSARYTLPLAVVPAVLVQHCCTIAFYYLCDQRASDQARDRYREALAWLKDVMN
                     GNVPVGVDTDGAAPESGDLPQVQSDAAVFGRNQKGFI"
     gene            26444..27007
                     /locus_tag="OO544_001184"
     CDS             26444..27007
                     /locus_tag="OO544_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1834 family protein"
                     /protein_id="HCR6766829.1"
                     /translation="MITETEQAYIARIREYFGNELVSVDTHPGDWSDSVLRTMLISAP
                     AIYVAWLGAGEGRTRGRLVSHWVFYVIGDMLNGREVSRPGLYQIVARLITVLDGFRTE
                     KTSPLYFEKAVNGYTETQADSGAVMYALYFSCEEMIAPLTDISSLDDFLRHYETFTEP
                     EGTPEFKAHISLPGATAAGEKAGPSEE"
     gene            27011..27298
                     /locus_tag="OO544_001185"
     CDS             27011..27298
                     /locus_tag="OO544_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000207437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HCR6766830.1"
                     /translation="MTTIFIKPAPGCLIRDPDTMKPLAQEGEEKPFTPFWCRRLNDGD
                     VIVVEKAAEAAPAAASATTDAAGKPAGAAAEKPATEKPATEKPAASDKGTS"
     gene            27298..28800
                     /locus_tag="OO544_001186"
     CDS             27298..28800
                     /locus_tag="OO544_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HCR6766831.1"
                     /translation="MINFDYIGDNNRIPLVQIEINNSMAVTGTPPQRQAVLLFGQAAM
                     KDTTIQGRGQLDVPVRITRASQARELWGRGSMIALMVEEFIAINPDTELYAIAQGVGT
                     GQSRACVMNIIGTALADGVLSVYIGGRRYLLPVSKGKKGKELVEELAKVINADTDAPF
                     TAIAAEVSGDNAEGLKGSMGVNARFIGECSAHDLRVNYYDGETTPAGLTLELVAPTQK
                     ATNPDITRSVSGMGNRQYNYIVMPYKDAANLKILADELLKRWGPVKMSDGMVWMAHTG
                     TFGTATSFGESRNDFLFTCTSIPKAPEPDYVWAASICAVCAPSLSTDPARPLQTLALP
                     ARMAPEAPARLTREERNSLLYTGMSTVTVAAADVVQIERQVTMYRQNAYGESDPSYLD
                     VETIYTLSYLRYSLRTFITQRFPRHKLADNGTPVRPGQNIVTPEIMTLQLIALGEEWV
                     ELGLVENLDTFKKNLLVERNTKDRNRLDVMCTPDLVNQFRFMAAQIRFIL"
     gene            28809..29174
                     /locus_tag="OO544_001187"
     CDS             28809..29174
                     /locus_tag="OO544_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HCR6766832.1"
                     /translation="MSGKQHQGTATIRVNGQEYATLEGATFSPSGFEREVVKGAKVYG
                     YREKPREATLDCKFPAGGEGSPAADEINTWTAVTIEFVADTGEVHMMTKAWSSEPASL
                     DGGGDISAKFASATSTRVQ"
     gene            29189..29668
                     /locus_tag="OO544_001188"
     CDS             29189..29668
                     /locus_tag="OO544_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HCR6766833.1"
                     /translation="MTTRKKKTAVSDEAVMEAIRDVLEDGDPRTAGLAEQLANGYVDL
                     LDGLPFGESREYRVTFRELTAKDSIDAETEAERLMETRNGGPVLIASPALRGIALLRR
                     QIAAVGQIEGPLSPRQIGQLSERDLSRLMAAVSLLDSAMAGKLVAERGRPDAVPGSD"
     gene            29796..>31358
                     /locus_tag="OO544_001189"
     CDS             29796..>31358
                     /locus_tag="OO544_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HCR6766834.1"
                     /translation="MAKNLKASLIVDLLGNISAKSRQWSQELGAFSRSGRTGLGGLGN
                     AARRAGQETELVGSRMQRTLAGVRGSIRTVTSDFDRLQGSITGTIGRISNLYGMLAGG
                     AAVYGFNKAFIRPAAEMENYILRLNAINHGDKAKTEAVKAWAVQNAKDTTWGLAGVMQ
                     EYASSRGFGMSDREARRFITMLQDQGGYHGWSLSEAQGASLQLKQMFARQSIQAADAN
                     ILTGYGINVYQLLADKLGVNQKIIREKGEKGKLGPDSIRLLFQVMAEQAKGAQKNAMN
                     SWTGMTSMMGDVWDQFARDVMAKGPFDSLKKSLKGFLDYADAAQKSGLQDKLATQTAS
                     ALNQGFEYARDAATGFYRAIQKVRETLQALRDAGYGDALDRIGQGAQTAAKYLMYMYL
                     ASRALKVLRFAGTGALRLGATPLRYGMAMTSVLTSPFRKPQTTVPGTQPGRAGRFLNF
                     LTGVNPAAVQPVLVTNWPAGGLASGGGDVVVSGDGKTVRGRKKRGPGRGRGVTTVVTA
                     GEQLAESAGKQGF"
BASE COUNT         7749 a         7873 c         8859 g         6877 t
ORIGIN      
        1 gacggtaaaa tcctcaccgc ggaacgttgg ggagcgatga acgtagaagt gaaggacggc
       61 aagattgttt cttcaacagg cgcgctggcg aaaaccatac cgaattcctt acagtctacg
      121 gcggcggatc aggtacacac cacgacgcgt attcagcatc cgatggtgag aaaaagctat
      181 ctcgataatc cactgcaacc gacgaaaggt cgtggcgaag atacctatgt acaggtgagc
      241 tgggaacaag cattaaaact gattcatgag caacacgatc gcattcgcaa agctaacgga
      301 ccatcggcta tttttgccgg atcatacggc tggcgctctt ctggcgtgtt gcataaagcg
      361 caaaccttac tgcaacgtta tatgaacctg gcgggcggtt attccgggca tagcggcgat
      421 tattcaaccg gggcggcaca ggtgatcatg ccgcatgtgg tcggttctgt agaggtgtat
      481 gaacagcaga cctcctggcc gctgattctg gaaaacagcc aggttgtggt gctgtggggg
      541 atgaacccgc tgaatacctt aaaaattgcc tggagcagta ccgatgagca agggctggaa
      601 tacttccatc agctgaaaaa atctggcaaa ccagtgattg ccattgatcc tatccgctcc
      661 gaaaccatcg acttctttgg cgataatgcc acctggatcg cgccgaatat gggcaccgac
      721 gtggcactga tgttagggat tgcgcatacc ctgatgacac aaggcaaaca cgataaagtg
      781 ttccttgaaa aatacactac cgggtatccg cagtttgaag agtatctgag aggtaagagc
      841 gataatacgc cgaagagcgc ggcctgggcg gcggaaatta ctggcgtacc ggaagcgcag
      901 atagtcaaac tcgctgaact gatggcggcg aatcgcacaa tgctgatggc aggctgggga
      961 attcagcgcc agcaatatgg tgaacagaaa cactggatgc tggtgacgct ggcggcgatg
     1021 ttagggcaga ttggtacacc gggcggcggt tttggtttct cttatcatta ctccaatggt
     1081 ggcaacccga cacgtgttgg cggcgtgctg ccggaaatgt ctgcggcgat tgccggacag
     1141 gccagcgagg ccgctgatga tggcggaatg agggcgattc ctgttgcccg tatcgtcgat
     1201 gcgctggaaa atcctggcgg caaatatcag cataacggta aagagcaaac gtatccgaat
     1261 atcaaaatga tctggtgggc gggtggcggt aactttaccc atcaccagga tactaatcgt
     1321 ctgattaaag catggcagaa accggagatg atcgtcgttt ctgaatgcta ctggaccgca
     1381 gctgctaaac acgcagatat cgtattaccg atcaccacat cgtttgagcg caatgacttg
     1441 acgatgaccg gtgattacag caaccagcat attgtgccga tgaagcaggc tgttgctccg
     1501 caatttgaag cgcgaaacga ttttgatgtg tttgccgatc ttgcggaatt actcaaacct
     1561 ggcggaaaag agatctatac cgaaggtaaa gatgaaatgg cgtggctgaa atttttctat
     1621 gatgccgctc agaaaggggc ccgtgcgcaa cgcgtcacta tgccaatgtt taatgccttc
     1681 tggcagcaaa ataaactgat cgaaatgcgc cgcagcgaga agaacgaaca gtacgttcgt
     1741 tatggtgatt tccgcgccga tccggtgaaa aatgcgctgg gtacgccaag tggcaaaatt
     1801 gagatttact ccaaaacgct ggaaaaattt ggctataagg attgcccggc acacccaacc
     1861 tggcttgcgc ctgatgagtg gaagggtacc gccgacgaga agcagttgca gcttctgacc
     1921 gcacatccgg cacaccgttt acacagtcag cttaactatg cggaactgcg taaaaaatat
     1981 gcggttgccg atcgtgaacc gatcactatt cacaccgaag atgctgctcg atttagtatt
     2041 gcgaatggcg atctggtgcg tgtctggaac aaacgtggtc agattttgac aggcgcggtg
     2101 gtgactgacg ggatcaaaaa aggcgtggta tgcgtgcatg aaggtgcatg gccagatctg
     2161 gaaaacggct tgtgtaaaaa cggcagtgcg aacgtgttaa cggcggatat ccccagctcg
     2221 cagctggcaa atgcctgtgc cggtaactct gcgctggtgt atatcgaaaa atatacgggt
     2281 aacgcaccga agttaacggc atttgataag cctgctgttc aggcgtaaga agtgaatagc
     2341 gcccggaaac gggcgctgag gttatgcaaa cgtaaaactg cctgatgcgc tatgcttatc
     2401 aggcctacat agtccctgca atagattgaa ttagcaaggg tttgtaggcc ggataaggca
     2461 ttcacgccgc atccggcagt aataccagac ctttacggct tgcgcgcaat cagcaccgcg
     2521 cgtttaggcg caggataacc ttcaactgtc ttactcggat catgtgggtc gaggaaatcg
     2581 gacagagatt cggtaaccat ccattcagtg cgtcgctgct cttcagtagt ggtaacgcac
     2641 acatccacaa tgcggatatc tacaaaacca cacttcttca gccagttttt cagcgccagc
     2701 gcggaaggga tgaaatagac attacgcatt tgcgcgtaac gatcgcctgg taccagcacc
     2761 gtgttctcat cgccatcaat aaccagcgtt tccagcacca gttcaccctc attcaccagt
     2821 tgatctttca actgccagag atgctccagc ggcgaacggc gatggtaaag cacgcccatc
     2881 gaaaagacgg tatcaaaggc tttcagtgcc ggaagctgtt caatacctaa cggtaacaaa
     2941 tgtgcgcgct gatcgttacc cagtagttta cgcaccgctt caaactggca cagaaacagt
     3001 tgcgtagggt cgatccccac cgcgaggtgc gcgcctgcgc caatcatgcg ccacatgtga
     3061 taaccgctgc cgcagccgac atccagaatg gtgcgtccgg ttaaatcaga aagatggggc
     3121 agaacacgat cccatttcca gtcggaacgc cattcggtat cgatgttgac gccatacagt
     3181 gagaacggcc ctttgcgcca tggcatcagg ttccgcataa gcgtttcaat gcgtttaatt
     3241 tgcccggtgc tcagtggctc ttcgctttcg gcggttacgc tatgcaataa atccagacga
     3301 tacggtttaa tttcaggcag aaattccacc gcgttggacc actgcttaaa cagcccgtgc
     3361 tgctgctcgc gctgccagtt agcaatctgc gcgggcagcg tttcgagcca gtgtgaaaga
     3421 tgatttttgg caatcagaga ataaaagtta ccaaagtcga tcatgcagcg tcctctgctt
     3481 ttaatgccac cagtgaacca aagttaaagc actggaacca cagctcgcta tgctcaaaac
     3541 cggctttatg caggcgtgct ttatgggttt ccacggaatc ggtcagcatc acgttttcca
     3601 gcatgctgcg cttctggctg atctccagtt cgctgtaacc gttggcacgt ttaaagtcgt
     3661 ggtgcatgtt gaacaacagt tcaccaactt tggcatcttc gaaactgaat ttttccgaaa
     3721 gcaccagcgc gccgcccggg ttcagtcctt gataaatttt atccagtaat gcctggcgct
     3781 cggaaggttc caggaattgc agggtaaaat tcagcaccac catcgatgcg ttttcaatgg
     3841 cgatatcgcg aatatcacct tcaatgacgt ctactggcgt aggggcttta taggcgtcaa
     3901 tatgacgact gcagcgttca atcatcgccg gggagttgtc gatggcaata attttgcaat
     3961 tatcatgatg aatgttgcga cgcaccgaga gcgtcgccgc gcccagagaa caacccagat
     4021 cgtaaacctg cgtaccaggt tgaacgaagc gctcggctaa cataccaatc atggaaataa
     4081 tattggaata gccgggaacg gaacgctgga tcatatccgg gaagacttca gctacccgtt
     4141 catcaaaggt ccagtcgccc agtctggcga taggggcaga aaatagcgtg tcgcggtgag
     4201 acataacgta aaaatccggg aaaaagaaag tggcgtattg tgcgctaacg cagggagaaa
     4261 accaactccc agggcatata ccaaagattc gccagcacca tcagcaacag cgcacaccag
     4321 gtggcgctca tgccggaacg tcgccagcga aacagacggt gatgaaaacc gtaataatgc
     4381 atcagacgac cagcaagcaa aacgatgccg caaatatgca ccatccaggt ttctgcgcca
     4441 ttcatttcca taaacagcat cagcacaatc gcgatgggaa tatattccac cgcgttacca
     4501 tgaatgcgaa tagcgctttg cagttcgcta aaaccaccgt caccataggc aacgcggtac
     4561 tgcattcgca ggcgaacgac atcaaaagag aacttcatta ataacaacgc acttaaaacg
     4621 gcatatagcg cgcttaccat acaaactccc tttaaaatgg ccgatggcac ctgtctatga
     4681 taggtggcaa attcagaaaa gagaagattg ctgtggaata gccggaactg ctccgatctc
     4741 tggaagcgtt ttacgtaagt cttcccacag ggtatgtacc agttccgggg cctgggcaat
     4801 gtctggcgta tgtaaaaaaa gataaggcgt agtggtctga tgccactgcg ctaatttctg
     4861 taaccagacc tgaaataatt cccggttttg cgtcatatca tcactaccga taaaacgaat
     4921 cagcggattt gtgtaaccag gtgaatcatt tgataccaac gagggtttta gtgaaaggag
     4981 gtgaaatcac ctgtaagata ctgaaataaa acaggtgatg tctggatgag cgcttaaagt
     5041 ctatttgata tcgattgtgc aataattaat cgtttattct aaaatcggca aaacttttga
     5101 tactgcgatc tcaacttttt aaagcgttta aaacttcatt aaatctatat ggcaactaac
     5161 atacgggcca gcctcgccaa actaacccgc tttagattct accggatttg attgcctact
     5221 tgtctcggtt ttttccaaaa tctctctgac gcaatcgtct ggcatatcta caatcatcaa
     5281 cgcagccagg cgcttcctgc cttctagttg taataaatcc tggttttctt tattcagcag
     5341 tgtcagaacc gtctctacac ctttgcgagc aaggagattg gttaactgtt ctcgttcgtc
     5401 tttcgtaagg aatgaaagca tctgtgtaag ctgatctccc ttatcaccag aacaaccaaa
     5461 attaccataa ataccaatat cgtttggtaa ttcacccttg ccctctttaa gccactcaag
     5521 actaacgcct tcactttcag aaattcgtat aaggatactt tctctggggg ctggttgtgt
     5581 ttgtcctccc ttgaaatagc tgtttaatgt gttgatattg attccccacg ctctggcagc
     5641 tgcacttctt gaagggtagc gctttgtaag cattccaata cgttcaataa caggttcttt
     5701 tccctgcgac ataaagatac ctcttggtgc ttttctaagg aaaggtttct ttctatttat
     5761 ccaattgaaa aataaggata aaagcaaaaa ttagccaaaa taccaaaaat accttgattt
     5821 ggtatttttg gtgatgcatt atttacccgt acagatatcc gcacgaatat ccgcgcgggt
     5881 aactttttag aatagaggaa gtatgagtag aagagaagtt tcagatcacg attggccgcc
     5941 tgaaatcatc aaggcgcgct tacacatggc tggcttatca ctgcgctctt tatctttaaa
     6001 ggctggttac agcagggatt cgctgaaaag cgtattacgt actccttgcc gaccgtatca
     6061 gcaaataatt gctgatgctc ttggggtatc acctgaagaa atctggccca gtcgatacca
     6121 ggttaagagc tatatgagaa aggcgtcatg atatgtatgt cattgcaaaa gaactgattg
     6181 gcgcgcccgg aatgcctgct acaacaaaag gtattcgcca ggcattacaa cgttacgtac
     6241 aagggaaaag ctgttgttcc cgtcgtcgct caggctctaa agcaactgaa tacagcatcg
     6301 actgtttacc tgaagtgacg cagcaggcat tacgtgaacg ctacgccctg caactgatga
     6361 cgcaaaaagc cgatgaatca ccggctccgg tggtgacaaa ggccagacgc tcacctgccg
     6421 tggttgatgc ggtggaggca tatcgcggat caccccaact gatggtcgaa cgcctcaatg
     6481 ccctgactga aaaccaacgt aaagtggcag aggcacgaat agcgatcgtc agcgaggtgc
     6541 tgaaaatcgc gcaacaaccc ggctttagtt gcgctaaggc tatccggttc ctcgttaaca
     6601 gcctggcacg atcacaactg gacgagcgca ttgtggcaat ggttgagacg gcgaacgcca
     6661 aaaagggaaa cagccgcgcg ttgagtgaaa tcacgctgaa acgctggatt gcggccttta
     6721 acaaggcaca gaacgccgct gaacgcctgc ttttactggc accgggtaaa cgccaggaaa
     6781 taaaagccga agatattaac tggctgcccg aatttctggc gcagtatcgc cagtcaaacg
     6841 gccgaccaat gaccgaggct tacgaggatt ttgttgctga atggcagcac cggcacgctg
     6901 atgagcctta tatgctcgat atcatgccct cttatgacac cattcgccgc gcaatgaaga
     6961 aactgccgga agtggtgaaa caaaaaggcc gggtaactgg cagtgaatac cgtcagcttg
     7021 agggattcac gcgccgcgac tggtccagaa tgccggtgaa ttatgtctgg attggtgacg
     7081 gtcacggcat gaagctgaaa tgcgcgcacc cggttcacgg gcggccattc gcaccggaag
     7141 tgacctttgt tatcgacggt ggcacgcgct ttgtggtggg ctggagcctt gacctggctg
     7201 aaaatgtttt cgccgtagcc ggtgccatac agcacggtat tcgccatcat ggcaaaccgt
     7261 ttctgtatta ctcggataat ggctccgggg aaaccgccga cgtcctggat aaggagattg
     7321 tggggatact gccacgactg gggattaatc acccgacagg gattgccggt aatccgcagg
     7381 gacggggcat tatcgaacgg cttaaccgca cattaccgat gcgcatagcc cgtaaatacc
     7441 gcacctattt cgggaaagga gcagatcgcg agacgttacg taaaaccaac cgcgatttac
     7501 gctcggcatt cagtgccctg caacagggca aacggctgaa cgcccggcag cagtcagcga
     7561 tgcgtgattt accgtcctgg tctgaactga ttgatgccat tcgtgacggt gttgagtggt
     7621 acaacaaccg gccgcacgat gaattaccca tgaagccgaa cggtaaacat tacagcccgg
     7681 cggagttcag aaaaaaacgc ctggcggaag aggacacgga aattgaatgg ctgtccgatg
     7741 ttgaactgcg ggatatgttc cggccgatgg tggagcgccc tgtaagacgc tgtgaaatac
     7801 gctggctgaa taatatttac tacgcgcccg agctgcgtga cgagcatggc cgtaaggtac
     7861 ttatcagcta tgacattcat gatgccgaac gaattaccgt gcgccgcccg gatggcagcg
     7921 tgatttgcga ggcggtatgg gacggcaata aacgcgaagc cttccctgtc agcgcggaat
     7981 actacaaaca gcagcagcgc cttaaaggca tgcgtaaacg cgcagaggaa aaaatccgtg
     8041 atgccgagga tgaggttgtc aacgtgctgg agcacaagcc gcaggagccc tggctggaaa
     8101 acatataccg ccctgtgggt aatacggtga ccgttcagca accggtcgct gacgatgaac
     8161 ctgatgaaga atacgagcgt aatttccagc ggggattgca actgctcgaa gcgaaattaa
     8221 aagaaagtga tccgctggcc tgaaataaaa aaataacccg aggggtgact caggttattt
     8281 gaggtttcag tctcggagaa tgctgtctca agcgaggtga ataatatgac cgatattaac
     8341 gatgtaatca agaccattga tgaacttatt gatggcggcg tactgacgca gtatgccatc
     8401 gccagagagg cgggaatttc cgacggcaca ttatcggctt tccgtaaggg aaaatataaa
     8461 ggcgataacg ctgctgtggc tgcttccctg cgctcctggt atgagaactg gaataaacaa
     8521 agcgcactgc cggaaccgcc gcagtttgtg gaaactcaga cagtccagga gctgcgcgca
     8581 ctgtttcagg cggttcgcct gatgggctgt attaacgtta ttgtgggcgt accgggtgtg
     8641 ggtaaaacgg ccactgcccg taattactgc caggagcaac caaacacctg gatgatcacc
     8701 ctgtcacccg cgcactccag cgtcacggag tgtctgctgg agctggccga tgcgctgggg
     8761 attgattaca cccgcgcgaa caaaggggca ttatcccgcg ccatccgccg tcgcctgatg
     8821 ggaacgcgtg gactggtgat tgtggatgag gcggatcatc ttggtattga cggtctggag
     8881 caactccggg caattcagga cgccacgggg atcgggatgg tgcttattgg taacccgcgc
     8941 ggattgttta aaggtggacg ccgcgccttt gatgatttat cgcgcctgtt cagccgtctt
     9001 gcccgtacaa aacaacttcg caaggccaaa aaggcggatg tgctggccat tgccatggcg
     9061 tgggggatca gtggtgaggc cgagctggcc gtcatgcagg ctatcgctga aaagccggga
     9121 gcgttacgcg ttctgacaca tacgcttaac caggcgtggc tcaccgccag cggtgaaggc
     9181 gcggcgctga cagaaaaaca tattaatgcg gcctttaaag aggtttatac caaccctgaa
     9241 ttactctcac aggtgtgatt atggcggtat ttaatattcc tgatatttac ggacgctttt
     9301 acctggttaa tttcgataac gtgaaggtga tttcactggc cgaaaataaa gaatgtggcg
     9361 atttactttt tgaatttaat gaccgcacac gaatggtgat atctgccgga cttgatcgcg
     9421 aaggtgcgac agaagtttac agcggaatat gccgttctgt tggtgcgaaa caagtcagct
     9481 aaatgaggtg ttatatgaat atgcaatcct gcggtaacaa aatgaattta ttcgactccc
     9541 tgaacagcgc gcgccgtctg accgaacttg ccggtgcggt actggaacgc agtaagcgct
     9601 atccgcaacg ttttgcactg aaaaccacgc cgccggtagg caacgtgcag ggaaccggtg
     9661 aaattgaaat caccatacag accaacggcc tgcgccgccg tgtgaaggcc acccgcatca
     9721 gcggctgcac ggtttactgg gaggtgtgag gtgaaaaaaa atctcattgc atgggcgtgg
     9781 tcgagtggtc ttattgagtt tggttacgtc ctgccggaag gtgcattgcc gatagttgcc
     9841 ggaaagcctg ccacggtacg gcatgtgatt gaggttatgg cgcgtcatgg acgtgatgaa
     9901 caggagcagt tactggttcc ggggataccg gaagcggtga cggaggaaga agcctttaat
     9961 gccatgattc ggttctgccg tgaggttaga cgccgggtca gttatccaaa cagaacgagg
    10021 accagaggat gagtaaagtc gtacgcatta ttttcgaata caaggaagat gttatctgca
    10081 aaaactctga tggcagcacg cacagagggg taagtctgga tatacgttca accggaataa
    10141 agaagaaaga taatggaccc gccatgattt ttggggtggt tatgctggca gaaagcagaa
    10201 acttcgcaga gtttgtggca atgaaagcca gtgcattcat gaaagacaga ggcatggctt
    10261 ccggggttat taacggtaat gaatttaatc aacaggggta attccatgag caaagtacgc
    10321 gttatttttg aatttaatca tgtttcgcat gacgaaaagt tggcaggcaa tgactgtgtt
    10381 gaagtgcatg aaaagattgg agtggatgtg aaaacagaac gtgatacgga taacaggccg
    10441 acgtcactct gtgacgttta tgcaagtatt ctccagtatc acagtcctgc aattattcag
    10501 tttctctcag cggaatttca ggcatctgca cagtcttttg gagcggatgc catcattaaa
    10561 cgccaccgcg tgcataaagc atcaggcaca ctgcaataag gaaaaacaaa atggctaaac
    10621 gcgttacaaa attaaaggcc gcagccgagg cggcaccgca gacccgggaa gaagtcagcc
    10681 gtgatatccg caccctggga gatattcagc gagaggcgct gcgcctggaa acggcgatga
    10741 atgacgaagt ggcagaaatc accgcccgtt atacgccgca gattgaaaat cttaaaaagc
    10801 aaatcaaagt gctttttaaa gggattcagg actggtgcaa aaccaaccgt gatgagctga
    10861 cgaacggcgg caaaaccaaa acagccaatc tgaccaccgg aacggtgtca tggcggctgg
    10921 gaacgccatc atgcagcgtc agccgtgatg tggaaggtgt gattgaaatg ctgcgccgta
    10981 tggggcttga gcgtttcatc cgcacgaaag aggaagtgaa caaggaagcc gtcctggcgg
    11041 agccggatgc tgtgaaaggc attgccggta tcaaggtgaa taaaggcgct gaaagttttt
    11101 atgtcgagcc ttttgaacag gacgccggac tgaataaata acaccgcatt aatcaattaa
    11161 atatcacttc attttaatta tggcgctcgc gtcaggggac tgcttgcgcc tgaaaacaga
    11221 aaataagggt taagaaatat gaaatacgtt tatatcgtca ttaataatgt ggtggggaaa
    11281 taaatcatga ttgatgcaaa agtgcttgaa ggggttaaaa actggctgtg tatttatggc
    11341 cgcctgacct gcggtgtact ggctgaaaaa atgaatatgc cgccatcctc catggtttat
    11401 tttttgcgtg atgcggttga tgcaggcgtg ctgacggaat gtaacggttt ttatgatatt
    11461 ccgcgtcccc gcccggtgca gccggttcgt cgcaaatgca gccaggaatc tgcggctgat
    11521 gatgttcagt ggtgcggctt cagaaaatcc ctgccatgga ttgaggggca tgatattccg
    11581 tcgatggcgt gggaatttgc tcagggcgtt ctgacctgtg aaaccgttta tgtggtggct
    11641 gaagttgatg agcaggccat gaaagaaggc gtgccccagt ttgtgatggc gtatatcgac
    11701 atacgcctgg gcgtcattat ctgcggttta agcggctgga atatcactga acatgttctg
    11761 cgctacctga ttgttgaccg gacagcagcg cctgccggga tatctgcgga ggtggcgtaa
    11821 tgttctttaa aacatcaaac ccttccgcgc tggctgcgtg gcaaaaatac cagcaggact
    11881 gccagaaagt taaggatgag gcaaaacgcc ttgaggccgt gctgaatgtt gcgtgccggt
    11941 cggtatttgt atccggtatc agtggatttt actttaaggg actgcgtttt acggaggaca
    12001 aatatccttt tcatcgcgac ttatggcgaa aaccgactgc gtcgaatggc tggagctgca
    12061 caccgcgcac atcacgtatc cctaaaaccc tgcgtgttgc ctctgatgaa ctgaacagtc
    12121 tgtggcgtga atattcgccc gtcacgtatg ccagaaccga tgcgctgttg ttctggttgg
    12181 gtattgattt ctcggcaatc ctgtttggcc ctgtgaagtg gttctgcgtt gacgatgtga
    12241 tttaccttca gtgcggcgta aaacccgcaa aacagaaaat gaccgaaata ctgtctgatg
    12301 agttttatgc tgctgaaaag cgagtcaggg ggtgatgcat gatgaaatta caacccatgg
    12361 gactaaaagg tcgcgccccc gctcatgtcc gtccgtggac acctgaagaa gatgcgctgc
    12421 tgattgcgct ttattcatcc accccggtta aggatattgc tgccagaata aaaagaactg
    12481 tctgggctgt atataaccgg actggtgtat tgcgcagttc atacccggag ttactgaaat
    12541 ataaacaccc aagatttaca cctgatgaag ataagtttat tcgaaaaaat gccagaacaa
    12601 tgacctgcca gcaaatggga gaatatctcg gacgtaataa agattctgtc agatgtcggg
    12661 caggaatgat tggtgctgga ttaacaaagt gcggagagtt acgccccgga acgcacatat
    12721 ctgatgatga tgtacgtctt atacgtgcgc tgcgtgattc cgattaccca cgccgtctgt
    12781 cattccggga aattggcgaa aagtttggaa tatctgaaca ttctgctcac gcagtttatt
    12841 accgtcgccg gactgccgag gacgctgtat tacgggagtt aacgccatga taacgacctt
    12901 atttgttgaa tcagatgaac ctcttgtgtg tgccgccgga atgccgctct gtggcggaac
    12961 gctgaccggt gtttatttcg gggatttacg cggttatccc tggcattcac tgaatgatgc
    13021 tttcccacct gatatggagg ctgccgtgct gattgttcag tatggtcacc gtcaggagct
    13081 gcgcatcggc catatggggt atgaaggctt ttttgttgat gaagaaaccg gagcctgcct
    13141 tgaagatgaa gacggacagg tgacgcactg gtgtcatatt tcggctttac cggaattacg
    13201 ggaggtgaat gcgtgagaag ttatattgat aatgaaaagc tggagacaat aagcgactgt
    13261 cttagcttgc tggcaaaaat caaagaaact attgaagaga ttaaatttca gcttgaatac
    13321 gcaccatgtg gcgatgatgc atggcgtaat gctgccagaa aagcactggc tgtctttcag
    13381 aaacaacgcc gggctgttga ataccgcctt gctgtattgc gccaggagga aaaggaaaga
    13441 aatataagac gtcatgaacg cgttaatgat ttccttgttc gtgaattaaa agagcgcgtg
    13501 ccggagtcgg tgttttttga atgtgaggct atagcccgta gcaaagcact ggaaacagat
    13561 taagcaggca ggtgaataat gagtattgaa ttaagatcgt cttatgaata tcgcaaaatc
    13621 cttatcgccg gaggcatgaa accggaagat gcagagaaat tcgtttcttt tatggataaa
    13681 gaatgtgaca agcgggatat gccagaaatt attatggatg acatgattct ggattcagcc
    13741 gtggcgttaa gtccgttatg gattgtgcat actcttgcgg aaatagctaa aagaagtgac
    13801 aagcaggcgg ctgtcgccgc cctgcgatta ctggctgaat taagttgttc agtcaatccc
    13861 aatacgttct ttgacgaacc tggttaacat tgatttggta acttctttaa ttagatcaaa
    13921 tggtgcgtct gtaaactcct ttttgattct ttcaaggatt ggtttttttc tcagggcgtt
    13981 agctaaatca tgcccctgct gggtaagcat aatgggcgtg tttctgaaac caacgcccag
    14041 agaatggaaa aagaaaccaa tatatttggg tgtttctgac tgcatatcac cattaacaat
    14101 tgagccatta tcagcgagaa gcataagatg aaaaactttt ttatcctttt cctctccttc
    14161 tgcctcagcc atgcccagat cctgtagtgt aaggaaaggt gtttcagcat cctgaaaagt
    14221 tgtgagaata cttaatactt catcgtaatc tattttcata tcgatacctt tagttttatc
    14281 cgggcggcgt tgccatgccg cctgttgtta cggagacacg ataatgaatc gcgcttccct
    14341 gattacctta atacatgtcg caaaacgcga tctacagctt gaccatgaga cctacacatc
    14401 cgtactactg gcagccaccg gcaaacccag ttgccgtgat atgtcacccg gtgagttatc
    14461 ccgcgtgctg gatgttttca aaaaacgcgg ttttaaagtg cgtcagaagc cggttaaccg
    14521 ggccttaaaa ccgggtacgg tgaccgccaa aattcgcgcc atctggaaag tgatgcaccg
    14581 gcagggattt atctctgatg gcggggaaac ggcccttaac cgctgggtga aatcgcagac
    14641 ggccgcgcag aacgacggcg aaggtgtggc taactggcag tggctggagc aacaacccgc
    14701 gctggcctct gatgtgctgg agcgtctcaa acgctggcac cgccgcaaaa tgctggcagc
    14761 cctggatatg tcggaacgtg cagtgatgag ttatgagcgt gtttgcaggc tgtatgaaaa
    14821 atcattcccc cgttaacccc aaatcccgcc acagcgcggg atttttattt taaacttgcc
    14881 ctgtgtaatg atgaccggag gcgggtatgg ctgaaacaca gatgagcatg tttggtgaca
    14941 gcgaacagct gcacgcgctg attgaccgtc tggatgatat tcctgatgat gagctgaaaa
    15001 agaactggcc cggcacgctg cgggacctgg ttgatgtgat cagtgctgaa ctgcaccgtc
    15061 aggggattga acctgctcag gcgggaatgc ttgcccgtaa ggttgcagcc gcacaggccg
    15121 ggtacatggg cgggcgtggt tattatctcc cggtcggtga atcccttttc accgaactgc
    15181 gcaataatga aattttttcc cgctggagcc agggcgaaaa aattgaaacc ctgcgccgcc
    15241 actaccagat gtcagagacg cagatttact ccgttatccg caaccagcgt cgcctgcatc
    15301 aggagcgcac gcagccccgg cttttctgat attccgcaaa tcctgccccc ttttcctcac
    15361 ggttacgctg actcagagaa caccatgaga cagcgtaaca atgccaaaac tccccgcacc
    15421 cctgcgtaaa aagctgattg cccttgttct ggccggagcc gggacgttca ccattgccac
    15481 gcactacacc ggctactggg aagggaaaga aaacaccacg tacattgatc ccaccggcac
    15541 ccccaccatc tgttacggcc ataccggccc ggatgtgaaa ccggggatga caaaaactga
    15601 tgaagagtgt ctggagctgc tggaaaagga catgaaatgg gcctttgcgg ctatcgatcg
    15661 tcatgttcag gtgccgctta cccgtggtca gacggtggcg ctggcctcct ggattttctg
    15721 ggccggtgaa acgaactttc gcaactccac gctcctgcgc cttatcaatg ccgggcagat
    15781 gcccgcgtcc tgtaagcagt atatccgctg gatttattca aagggggtaa aactccccgg
    15841 tcttgaggcc cgccgttcgg cggatgaatg gttatgtcgt tacgatttgc cgaaagtctg
    15901 aaccgcttct ggcgaccgct catgattgcg ctgctgtgtg ccgttgtgct gctgcggggt
    15961 gtgctgtgtc tgcggtgaac aatcccttac cggccctgat tgccggaggc tgtatggcgg
    16021 cgctgggggt gatgagcgtg ctggctgccg tggtatgggg aatgcaccag aagacgcagc
    16081 gccttgagga caataatcat gtgcttgtgc gtgaacggga cgaagcccgt ctggtgctgg
    16141 caaaccagca acgcaccctg caactcattt cacaaatcag caaggcggcc acgaatgaaa
    16201 aacagcagaa catgcagcac agcgaggaac agcagagcat tgtccgccgg tcactggcaa
    16261 cggtgcctgc tgccgctgtg cctgtgcctg atgatgtggc tgaccgggtg cggcgggccg
    16321 tctgtgaaat acgtgccggt gaagccggtg ccgatccccg ctgaatggct ggctgactgc
    16381 ctggtccctc ccgcgccgca gccgttcacg tttggtgcat cggtcactta caacctgcaa
    16441 ctgctggcgg tgattaaaaa ctgcaacgtg gacaaggcca gcatccgccg tctggagaca
    16501 cagcgacagc atgaatttac tgatatggcc ggaacgactg ctgttccggc aggaaagacg
    16561 aagtaaggaa aaaggtatgg atgattcaga ttgtgctcag gcagtcatgg agcgtgcaac
    16621 agaacgcgcc ctttgtgacc ggctgacacg taagcgccgg gtggcggaga caccgggcag
    16681 gcgtgtatgc gcggactgcg ggggtgacat tccggctgcc cgtctggttg ccgtgccgga
    16741 tgccatccgt tgtgtgaact gccagaacat catggaggcc ggtcatgtgg gtcagcatcg
    16801 ttaaagatta tgtcgtgccg atcctgtcgg caacggccac agccgggggg atcttcatgg
    16861 cgctgatgcg caaaaccttt gtcccccgcg aggcctttga aaaactctcc gaccgggttg
    16921 agcgggtgga gagccggatt tccggcctgc ccacgcagca ggaggtgaac agactcaata
    16981 tcgaaatcac caccctgcgc ggtgagctta aggccaccaa tgccaccctg aaatccgtct
    17041 cccatcagaa cgaactgctg ctggagcagg ctgtaaggaa aaaacacaat gagtgatttc
    17101 attactgaag atcagcgtct tgtcattctg cggtcgctgg cggattacaa cggcgaactg
    17161 ggcgaatccg tgcttcagga ctgcctggat gattacggtc accgcgtgtc ccgcgacacc
    17221 gttcataccc acattgcctg gcttgccgag cagggactgg tgcgtaagcg cacgcttgtt
    17281 aacggttatt tcatcgctga actgaccggc cggggtcagg atgtggcgga aggccgcgcc
    17341 accgttccgg gtgtgaagaa accacgcgca agggggtaac gatggataag ccagcgcgtg
    17401 gccgtgtacg caaggtggat ttgctccccg acagcatccg taagccgctg ctggaaatgc
    17461 tgcgtgaaaa acgtctgacg caggtccaga tccgcgagga aatcaaccgt ctgatccgtg
    17521 aagcaggtct gccggaagag cagcagctct caccggcggc catcagccgg gaagcctccc
    17581 gtaatgagct gattgcccgt aatctgcgtg atttacgcga gcagacaaaa gccatgatgg
    17641 ccgaactggg ggataagccg accggtgaaa ccacggcact gattctggag atgtcccgtg
    17701 ccctgatgta ccgccgtctg cgtgccgcca ccgaatccct gaacagcgac agcgatgtgg
    17761 atatgcgcct tattaaggac atcctcctgt cggcacagcg tgcagaaagt gccgccgagc
    17821 gcagcattaa gcgtgaaaaa gaaatccggg ccgcatttgc tgaagagatg gcgaatgcgg
    17881 tcaccgatga gttgcgcggc gtggatggca tgagcgagca gatcgaattc cgtatcaggc
    17941 agatgctgct gggtaaggcg taggaggtga atcatggcaa cagataaccg ctcactgatg
    18001 agcagtgccc atcagaaagc attcagtctt gcaacacagt tacctcctga aaccataaag
    18061 gtatgtcgtg agttgactga actcagcatt gccggggaaa tatccctgga acaactgcat
    18121 tcgctgctgg cagaggctgc cactgatgag ttgagcggcg tggacgtgat gactgaagag
    18181 cttgagtccc gaatcaaaag cattctgaca ggtaaagcat gatgaacaaa gccagagaaa
    18241 cgtcgccttc actgatgcaa atgacgcctc cccgcaaaat tgatctgtca ggtgaaaaag
    18301 ccctgctggg cgtggatgtg ccggacagcc ttgatttgcc cggtgatatg ccggtgtttc
    18361 tggattatca ggcccgctgg tttgcagatg aaagtcaggt ctgcatcgcg gagaaatccc
    18421 gccgcaccgg tcttacctgg gcggaagccg ggcgtaacgt cattactgct gcaaaaccga
    18481 agcgtcgtgg tggccgcaat gtgttttatg tgggatcaaa gcaggagatg gcgctggaat
    18541 atatttctgc ctgcgcgctg ttctcccgtg cctttaacca gctggcagat gctgatgtgt
    18601 atgagcagac attctgggat cgggataaaa aagaagaaat tttgacctac atgatccgct
    18661 ttccgaacag tggattcaaa attcaggcat tgtcttcccg tccgtcaaac ctgcgcggcc
    18721 ttcaggggga tgtggtgatt gatgaagccg cgttccacga gtcactggat gagcttctca
    18781 aggcggcaat ggcgctcact atgtggggcg cgcgcgtgcg tattatctcc acgcataacg
    18841 gcgtcgataa tctgtttaat cagtacattc aggaagcccg cgaggggcgc aaggattaca
    18901 gcgtccaccg cataaccctg gatgatgcca ttgcggacgg gctgtaccgt cgtatctgtt
    18961 acgtcaccga tcaggaatgg tcaccggaaa gcgagcagaa atggcgtgat gatctctaca
    19021 aaaacgcccc gacccgtgag gatgccgacg aggaatacgg ctgtatcccg aaaaaatccg
    19081 gcggtgccta tatcccccac gcgcttattg aaatggcgat gatccgcgac atcccgattc
    19141 tgacgtttga agccccggac aacttcatca gccgtgccgc atggctgcgt gaatcggaag
    19201 ttttaacctg gtgtgaagaa catttaaaac cgcttttaga gaaattaaat ccccgttccc
    19261 gcttcagctt cggggaagac tttgcccgca ccggtgacct gtcctgcttt gtgctgctgg
    19321 aaatcaccga atccctggca aagcgcgagg tgtttcgcgt ggagctgcgc aacctgccgt
    19381 atgcccagca ggaacaggtg atgatgtaca tcctgacccg cgttccggcg ctggtcggtg
    19441 cggcgttcga cgccaccggt aacggcggct atctggcaga ggccgcactg ctggcctttg
    19501 gcccggacat catcgactgc gtgatgttgt cgccgaagtg gtacggcgag tggatgccaa
    19561 aactgaaagc cgagtttgaa gatcagaata tcctcgtcgc ccgccatcag accacgcttg
    19621 atgatttgcg ccatgtgaag gtagtgaatg gtatcccgca aatcgacaag gggcgcacga
    19681 aggaccagaa cgcaacggct gcgaacgccc gccgccatgg tgattttgcc gtggcgctct
    19741 gtatggcaaa ccgggcgtca tacatggagg gcttcatcct ggatgaatca gcctgtcagg
    19801 cgctacagga gcggtcgcgg gcaatggagg gcggttatcg tgatgacgat gaggcatatc
    19861 atgaatttga tcgggggggc tggtagtggg acgcatcatt gatactgacg gaaaaccttt
    19921 ctcctttgac ccggagatgc agagtgccgc gctggatatt ccgcagattg ccagccgtta
    19981 tattgaacat ccggcttcgg gtatcacccc gaaccgggcg gcgcagtgcc ttcgcggggc
    20041 tgaacgtggc gatctgattg cccagtccga tctggccgct gatattgaag aaaaggacac
    20101 ccaccttttt gcggagctgg gcaagcgacg tcttgccatt cagggggtgc catggagtat
    20161 tgagccgcca ccgaacgcca gcgcgaatga gaaaaaggac gcggaaatgc tcgacgaata
    20221 tctgcattcc gcagactggt ttgatgccat gctgtttgac gccacggatg ccatcctgaa
    20281 gggctattcc tgcatggaaa ttgagcacgg gatgctcggt aaaatgcaca tcatccgcgc
    20341 catccgctgg cgtgacagcg ggcatttctg ccttaacccg gatgatttga gcgaactgcg
    20401 gctgcgtgac ggcagccatt ccggggtggc gtttcagccc tttggctgga tagtgcatca
    20461 gtcgcgttca cgcaccggtt acggtggtgc aaccgggctt gtccggacgc ttatctggcc
    20521 gttcattttc aaaaactatt ccgtgcgcga tctggcggaa tttctggagg tgtacggcct
    20581 gccgatgaag gtcgggaaat acccgtccgg ggcaacaccg gagcagaaaa gtgccctgat
    20641 gcgggcggtg atggatatcg ggcgacgtac aggcgggatc atcccggccg ggatgtcgct
    20701 ggagtttcag gcggcagcga acggtcaggc cgatccgttt gaaaccatga tttcgtgggg
    20761 ggagcgttcc atctccaaag caatactcgg cggcacgctg accacggaag ccggagacaa
    20821 aggcgcgcgc tcactgggtg aggtacataa cgaggtgcgc cgggaaatca gggattctga
    20881 tttacgacag ctggctgcaa ccctgaaccg cgatctggtg tatccgctgt acgccctgaa
    20941 caccacgcac actattgata tccgccgtct gccacgtatc tgcttccaga caaaagaacc
    21001 aggtgatatc accaaaatca ccagtgcggt gatgcagctc agtacgggga tggatatttc
    21061 tgatccctgg atacgtgagc agaccggtat tccgcagccc acgcccggtg aagccatatt
    21121 ccgtgtccgt cagagtggca atgaaccggc tcagaccgac agggaaacac caccggaaaa
    21181 acaggaaaaa acagagcaga cggcgctgtc agccagactg ccggaagcga aaagcacgcc
    21241 gcgtgatgaa ctggatgata tgggggacgc ggtgcctgcc cgccgcttac aggaggccat
    21301 cgacccggta ctggagccgg tcattgatgc catcagaacg cgggggctgg cggatgcgct
    21361 ggcagacctg cctgcccttt atcgtgaaat ggatgattcc cgcctgatga cgctgctcag
    21421 tgatgccatg tttgctgcgg aaatgaaggg gatgctggat gacacagggg attgatttag
    21481 gttatgccgc cacgcttccc tcaaaagagg cggtagcata cttccgtgtc aaaggggcgc
    21541 atatcagctg gaactggttc gaaacagccg cggatgttca tgcccgctca ttcacggcag
    21601 caaaagcggc acgcctggat gtggtgacca cgttacaggc cgaagtgcag cgggccattg
    21661 atgagggaat ttcacagaaa gcatttatcc gcacgctgac gccacgcctg caaaagctgg
    21721 gatggtgggg aaagcagatt gtggtggaca gcgacggtaa cgcggaagaa gtgcaactgg
    21781 gcagtccccg tcgtctggcg ctgatttaca acgtgaacac ccgggtggct tacaatgccg
    21841 ggcgttacac gcagatgatg aacaacacgg acacgcatcc gttctggcag tatgtggcgg
    21901 tcatggacag ccgtacccgc ccgtcgcatt ccgcccttaa cggtctggta ttccgctatg
    21961 acgatccgtt ctggaaaaca cactacccac ctaatggctg gaactgccgc tgccgtgtgc
    22021 ggccattgtc tcaggctcgt ctggatgcaa tggggttatc cgtttcatcc ggtgaggatc
    22081 atctctccac ccgcaatgtt gaggctggcg tggataaaca gaccggagaa gtcagagaaa
    22141 tgccggtgac cacatattca gatggcacca gaaccatgac accggatgtg ggctggtcat
    22201 ataaccccgg ctcggcagcg ttcggcacag accaggcgct gatccgcaaa ctgatcgagg
    22261 tgaaaagccc ggcgttacgg gaaatggtgg ttcaggagat gaacaacagc ccggagcggc
    22321 agctggcgtt ccgcatctgg gcaaaaaaca tcatgcagac ccggcgaggt ggtaacgata
    22381 tccgcacgct gggctttatg actgaaagca ttgcgcaggc agtggaaagc cggacgggaa
    22441 cgccaccggc ccgcctgctg gcgatgagcg gtaaaaatgt gctccatgca gacagtgtga
    22501 aacatcagaa tgacggcatc gccctgatgc cagaggactt cgggcgctta ccggcaatgc
    22561 tggcaaagcc aaaagcggtg ctgtgggaca aacggcacaa aaatctgatg tatatcgtgg
    22621 aaaataagga tggcagtgtg cagattgccg ttaatgcacc gtacagtctg aaacgtcagc
    22681 ccgacaaact ggatgtgatt gtgaatgctt accgggtgat caatatggat aagctgaaat
    22741 cggatatccg gggcggaatg cttgaggtgc tggaaggaca aattgattaa cggtcagtgg
    22801 cgggggtcga acccgcatac atgatccacc cggaggcagg caccgacttt accagttaag
    22861 cgtacactga ccgcctgttt attttatatt catcctttat gcggaggcaa tatgtcatcc
    22921 attgatgccg ctgtcgttgt tgacgttacg cgtctccagc gggtgtttgc ccggcttcag
    22981 tttgtgggcg gcggaaaaga cctggcccgc agtgtggcgg cgagtcttct gtcatcgtca
    23041 gaaatggcgt ttgaacagga aaaagagccg gacggcgaac gctggcatga ctggtcagat
    23101 ccttaccgca agtggcgtac ccgtaaggga tacacgcccg gcaaaatcct gacactgaac
    23161 ggcgatctgg cccgccgcct gaccacggat tatggcgata cctgggcgct gattggatca
    23221 aatgaacctt atgcggccat tcatcagtgg ggtggcctgc ctggtatgcc accgggaccg
    23281 gcggccattg gcgcacgtcc gtatatgggc tttgatcagg tggcagaaca ggagatcatg
    23341 gacgaaatca gaaaacgctt taaaaaggcc acagaaacgc cgtaatggtt taaggtatac
    23401 aaacgcatta ccttaccctc ttcaggcgcg tgtcgtgatt ttctaacctg tatttaacgg
    23461 gctttaaaat ctgcgtgaag cgcctttctc atttttcccc tgtcagcact ttctgaagtc
    23521 ccgcaaaacc ggccccgaaa aacaccggct tatgctgccg gaatgaagac gaaaaacacg
    23581 cccaaacttg cttatgccat tctgaatgcc atcagcctgt ccgcagacgg ggacggtgac
    23641 tggtgtcaga tcatgccagc cggtcgggta aaggcccgtg acggtcgccc ggaaaaaccg
    23701 gcggaaggct ggctgattaa ccatgcggcc gttgagcgca tggtatcccg ggttgtggcg
    23761 ctcaatcagc cggtaaaaat tgactacaac caccagaccc tgattaaggg gcatccggca
    23821 ccggctgccg gttttgtcat ggcctcaccg gaaaacttcc gcttcagtga agagcggggt
    23881 tttgaagtgc gtccggactg gaaccctccg gccattgaac acctgcgcaa taacgaattt
    23941 ccctggtttt ccccggtgat tggttatgac gaaatcaccg gtgaacctgt tgagcttcgg
    24001 atgctggcta ttaccggtga ccccggtctg actggcatga atcctgtcgc cgcactgtcg
    24061 gcggatgacc tttataacgc cttaaaccct cctttaaagg acacctccat gaatgagcaa
    24121 ttacgccagt tgctgacggc gctcggtctg accgtggccg acggtgacga atttacaccg
    24181 gaactgggca cggcggcgct gtctgccctt accgggatca aaacccgtgc ggatgcgcac
    24241 gacaccctga aaacgcaggt tgccagcctg tcggcagagc tggaaaccgc aaaaggcaca
    24301 ccaactggcg gcactattga tctgacgaaa tacgtgcccg ttgagacgta taacgccctg
    24361 cgtaccgaat atgtcgcgtt gtcggcacaa cacggcagca ccacgctgga gcagttgctg
    24421 gataaggccg agtccgaagg acgcattttc aaaagcgaac gcggatacat ggaaggactc
    24481 ggacaacaga ttggtgttgc ggcactttct gcacagcttg acgcccgcca gccggttgcg
    24541 gctctgacca ctcttcagac cgacaccgta accgtgccgg ataaaaagac cgccaccgct
    24601 gcgctgtcgg ctgaagatat cgccgccgcc catctgctgg gtaaaaccga agccgagttc
    24661 ctgaaaatga aagaggaaat gcaataatgc ctaccccgat cacaccggcg atgatcaccg
    24721 ccctgatgac gggctaccgt tctgattttc aggccgggat gtccatggct ccgtctcagt
    24781 accagaaaat tgcgatgacc gtaccgtcca cgtcgaaatc caacacctac ggctggctgg
    24841 ggcagttccc gcagttccgt gagtggatcg gctcccgcgt catcgagaag atgaaagcct
    24901 atggctacgc catcgtgaac aaaacctttg aaggcaccgt tgccattagc cgcgatgact
    24961 ttgaagacga caatcttggc atctactccc cgttgttcca ggagatgggg cgcgctgcgg
    25021 cggcacaacc ggatgaactg gtctttgctg cactgcgtga tggtctgaat gcggcctgct
    25081 atgacggcca gaacttcttt gataccgaac accctgttta ccccaaagtg gatggctccg
    25141 gtgatgcaca gatggtcagc aatatgtttg tggcaaaaac cggctctgtc ggtgcacagg
    25201 cggattacag cggcccggcc tggtatctgc ttgactgctc ccgcgcggta aaaccgctga
    25261 tttatcagga tcgccgcaag gccgaactgg ttgcccagac aaaagtcgat gaaggccgtg
    25321 cgtttactga taacgagttt gtgttcggtg cttccgcccg tcgcaatgtg ggctacggct
    25381 tctggcagat ggcctacatg atgcagtcac cgctgacgct ggatgcgctg tggcacggct
    25441 ggtcagccat gcgtgacttt aaggctgacg gtggccgcaa actcggcatc aaacccaccc
    25501 atattgttgt ccccacctcg ctggaaaaac aggcggtgca gctgctggag cgcgagctgt
    25561 tcgcggacgg taacaccacc gtctctaacg agatgaaggg caagctggag ctggttgtcg
    25621 cggattatct gtaaacggtg agccgggctt acggcccggc ccctgcggag ggcaaatatg
    25681 aatgaacatc acactgtggc agcaacagat gattcaggcc ttcaggtcag tggtgacagt
    25741 cctgtcacgg tactggcaga agtgcgttgc agtcgtgcgc agtttcgtcg tgcggggttc
    25801 ctgttcatgc ggggacgcca gcaggttgac gtcaccccgg agcagcttgc ccgactggaa
    25861 gcggagccat gcctcactgt acgaatacta cagacgcctg gtgatgatgc gggagccgtg
    25921 gcgggtgtgg ttcatgcaga gaccggtgca gatttagccg aagccggaca ggatgctccc
    25981 cggaaaaaga ccggcaataa cgccagacag gcgaaaaagg ctcgcgcatg aattacgcca
    26041 ccgaaaccga tatgcgggca cgttaccgcg aggatttgct caggccatta ctggccgtgc
    26101 cccgttcgga tgagccggac gcgcgcaagc tgaaccgggc actgacggac gcgtctgccc
    26161 ttatcgacag ttacctgtct gcccgttaca cgctgccgct ggcggtggtt ccggcggtcc
    26221 ttgttcagca ttgctgcacg attgcctttt attacctgtg cgatcagcgg gcatccgatc
    26281 aggcgcgtga ccgttaccgt gaggcgctgg cctggctgaa agatgtcatg aacggtaacg
    26341 tgccggtcgg cgtggataca gacggtgcgg cccctgaatc cggggattta ccgcaggtgc
    26401 agtctgatgc ggcggtgttc gggcgcaacc agaagggctt tatatgatta cggaaaccga
    26461 gcaagcgtac atcgcccgta tccgtgagta tttcgggaat gaactggtgt cagttgacac
    26521 gcatcccggc gactggagcg acagtgtgct gcgcaccatg ctgattagcg ccccggcgat
    26581 ctacgttgcc tggctgggtg ccggtgaagg ccgtacccgt gggcgtctgg tcagccactg
    26641 ggtgttctac gtcatcggcg acatgctcaa tggccgtgag gtcagccgtc ccgggcttta
    26701 tcagattgtg gcccgcctga ttaccgtgct ggacggcttc agaaccgaaa aaacctcacc
    26761 gctttacttt gaaaaggcgg tcaacggtta caccgaaacg caggccgaca gtggcgcggt
    26821 gatgtatgcg ctttatttct cctgcgagga aatgatcgca ccgctgactg atatcagctc
    26881 gctggatgat ttcctgcgcc attacgaaac cttcaccgaa ccggaaggca caccggaatt
    26941 taaggcgcac atcagcctgc caggtgcaac cgccgccggt gaaaaagccg gaccgtcaga
    27001 ggaataacag atgacaacca tttttatcaa acccgcgccg ggatgcctga tccgcgatcc
    27061 ggacaccatg aaaccgctgg cgcaggaggg agaggaaaaa cccttcaccc cgttctggtg
    27121 ccgccgtctt aatgacgggg acgtcattgt ggtggaaaaa gcggctgaag cagcaccggc
    27181 agcagcgtct gcaacgactg atgccgccgg aaaacccgcg ggagcggcag cagaaaaacc
    27241 tgcgactgaa aaacccgcga ctgaaaaacc cgcagcatca gacaagggga cgtcctgatg
    27301 attaactttg attatatcgg cgataacaat cgcattccgc tggtacagat tgaaatcaat
    27361 aattcaatgg ccgtgaccgg cactccacca cagcgtcagg cggtactgtt gtttggtcag
    27421 gctgcaatga aagacaccac cattcagggg cgcggtcagc ttgatgtacc agtacgtatt
    27481 acccgagctt cacaagcaag agaactctgg gggcgaggtt ccatgattgc actgatggta
    27541 gaggaattta tcgccattaa ccctgacaca gagttatatg ccattgcaca gggggttgga
    27601 acaggtcagt ccagggcatg cgtgatgaat atcattggaa cagcattggc agacggggtg
    27661 ttaagtgttt acatcggcgg tcgccgttac ctcctgcccg tcagtaaggg gaaaaaaggt
    27721 aaagagcttg tggaagagct ggctaaggtc attaacgctg acactgatgc gccattcact
    27781 gctatcgcag ctgaagtcag tggtgataat gctgaaggac ttaaagggag catgggggtt
    27841 aacgcccgct ttatcggtga atgctcagct catgatttac gggtgaacta ttatgacggt
    27901 gaaactacac cggccggtct tacgctggag ctcgtcgcgc caacacagaa ggcaacaaac
    27961 cccgatatca cccgtagcgt ctcagggatg gggaatcgtc agtacaacta catcgtcatg
    28021 ccctataagg atgcggcaaa tctcaaaatt ctggcggatg aactgctgaa acgctgggga
    28081 ccggtaaaaa tgtcggacgg tatggtctgg atggcgcata ccggcacctt cggtacggca
    28141 accagttttg gtgaatcccg taatgacttc ctgtttacct gcacttctat tcctaaagca
    28201 ccagagccgg attatgtctg ggcggcctcc atttgtgcgg tctgtgcgcc ctccctgtca
    28261 acggatccgg cccgaccatt acagacactg gcgctgcctg cccgtatggc acctgaagca
    28321 ccggctcgtc tgacgcggga agagcgaaac agcctgcttt ataccggcat gtccacggtg
    28381 accgttgcgg ccgctgatgt ggtgcagatt gaacgtcagg tgacgatgta ccgtcagaat
    28441 gcatacggcg aaagcgatcc cagctatctg gatgtagaaa ccatctacac cctgtcatat
    28501 ctgcgttatt ccctgcgcac ctttatcacg cagcgtttcc cgcgccacaa actggccgat
    28561 aacggtactc ccgtccggcc cggtcagaat atcgtgacgc cggagattat gacgctccag
    28621 ctgattgcac tgggggaaga atgggtggaa ctcgggctgg tcgaaaatct ggacaccttt
    28681 aaaaagaatc tgctcgtgga gcgcaacaca aaagaccgta accgtctgga tgtgatgtgt
    28741 acaccggatc tggttaacca gttccgcttc atggccgcgc agatccgttt cattttgtga
    28801 ggtaaggcat gagcggaaaa cagcatcagg gcacggccac catccgtgtg aacggacagg
    28861 agtacgccac ccttgaggga gccacgttct ccccgtccgg ctttgagcgt gaagtggtga
    28921 aaggcgcgaa agtctatggc tatcgtgaga aaccccgtga ggcgacgctg gactgtaaat
    28981 ttccggctgg cggagaaggt tcaccggctg ccgatgaaat caacacctgg actgcggtca
    29041 cgattgagtt cgtggccgat accggtgaag tccatatgat gacgaaggcc tggagcagtg
    29101 aaccggcctc gcttgacggt ggcggtgata tctccgcgaa gttcgccagc gccaccagta
    29161 cccgtgtcca gtaatcagga aaaatgcaat gaccacacgt aagaaaaaaa cggcggtttc
    29221 tgacgaggcc gtgatggagg ctatccggga tgtacttgaa gacggcgatc cccgcaccgc
    29281 cgggctggct gaacagctgg cgaacggcta tgtggatctg ctcgacggtc tgccgttcgg
    29341 ggaatcccgt gaataccgcg tcaccttccg cgaactgacg gcgaaagaca gcattgacgc
    29401 ggaaaccgag gccgagcgtc ttatggaaac ccgaaatggc ggcccggttc tgatagcctc
    29461 gccggcactg cgcggcattg cactgctgcg ccgtcagatt gccgctgtcg gccagattga
    29521 ggggccattg tctccgcgtc agatcggaca gctcagcgag cgcgatctct cccgtctgat
    29581 ggcggcggta agcctgcttg attccgcgat ggcaggaaag ctggtcgctg aacgggggcg
    29641 accagatgca gtgccgggat cagattgaag aagccgcaat tgtgctgggg atggtgacaa
    29701 aaagcggccc ggagtgggcg cttaacctcc ccttatcgca gctttaccgg cactgccgac
    29761 agaccgaaaa aattctcaga acgaagcagt aacccatggc aaaaaacctg aaagcctccc
    29821 tgattgtcga tttactcggc aatatttctg ccaaatcccg ccagtggtca caggagctgg
    29881 gggcattctc ccgttccggc aggacggggc tgggtggtct tggaaatgca gcacgtcgtg
    29941 ccggtcagga gacagaactt gtcggctccc gtatgcagcg aacgcttgcc ggtgttcgtg
    30001 gcagtatccg cacggtgacg tcagattttg accgccttca gggcagcatt accggcacca
    30061 tcgggcggat aagcaacctt tacgggatgc tggcgggagg ggctgcggtc tacgggttta
    30121 ataaagcctt tatccgcccg gccgcagaga tggagaacta tattctgcgt ctcaacgcca
    30181 ttaatcatgg cgataaggca aaaacggaag cagtgaaagc ctgggcggta cagaacgcca
    30241 aagacaccac ctgggggctg gcgggggtta tgcaggaata cgcctccagt cgcgggtttg
    30301 gcatgagtga cagggaagcc cgtcgcttta ttaccatgct tcaggatcag ggcggctatc
    30361 acggctggtc actgtcagag gcacagggtg cgtccctgca actcaaacag atgtttgccc
    30421 gccagagtat ccaggcagcg gacgccaata ttctgaccgg atacgggatt aacgtttacc
    30481 agttactggc cgataagctg ggtgtaaacc agaagatcat ccgtgagaaa ggcgagaaag
    30541 gaaaactggg accggacagc attcgcctgt tatttcaggt gatggccgaa caggcaaaag
    30601 gtgcccagaa aaacgccatg aactcatgga cgggaatgac gtccatgatg ggtgacgtct
    30661 gggatcagtt tgcccgtgac gtgatggcga agggaccgtt tgacagcctg aaaaagagcc
    30721 tgaaaggctt tctggattat gccgatgcgg cacagaaaag cggcttacag gataagctgg
    30781 ccacacagac ggcctcagca ctgaatcagg gatttgagta tgccagagat gctgcgaccg
    30841 gcttttaccg ggccattcag aaagtcaggg aaacgcttca ggcactgcgc gacgccggtt
    30901 atggcgatgc gctggatcgt attggtcagg gtgcgcagac cgccgcaaag tacctgatgt
    30961 atatgtatct cgcctcccgt gcgctgaagg tgctcaggtt tgcagggacg ggggcattac
    31021 gtctcggcgc aaccccctta cgctatggca tggcgatgac ctccgtgctg acgtcgccct
    31081 tccgcaaacc gcagaccact gtaccgggaa cgcagcccgg acgcgctggc cggttcctga
    31141 atttccttac cggggtaaat ccggctgccg ttcagcccgt gctggtcacc aactggcctg
    31201 cgggtggcct ggcctcaggt ggcggggatg tggtggtcag cggtgacggc aaaaccgtgc
    31261 gcggccgtaa aaaacgcggc cccggacgtg gacggggtgt cacgaccgtc gtgactgccg
    31321 gtgagcaact ggcggagagt gccggtaaac agggcttc
//
LOCUS       DAJTZL010000029        31179 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.029, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000029 DAJTZL010000000
VERSION     DAJTZL010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31179
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.029"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(1..870)
                     /gene="gabT"
                     /locus_tag="OO544_001190"
                     /pseudo
     CDS             complement(1..870)
                     /gene="gabT"
                     /locus_tag="OO544_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309906.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
     gene            complement(908..2188)
                     /gene="puuB"
                     /locus_tag="OO544_001191"
     CDS             complement(908..2188)
                     /gene="puuB"
                     /locus_tag="OO544_001191"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HCR6766835.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTAKGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARDPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPYYPFPGGRTLRVPFTAMGAAWYSLRDRLGV"
     gene            complement(2190..3680)
                     /gene="puuC"
                     /locus_tag="OO544_001192"
                     /pseudo
     CDS             complement(2190..3680)
                     /gene="puuC"
                     /locus_tag="OO544_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415816.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
     gene            complement(3830..4387)
                     /gene="puuR"
                     /locus_tag="OO544_001193"
     CDS             complement(3830..4387)
                     /gene="puuR"
                     /locus_tag="OO544_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HCR6766836.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(4414..5178)
                     /gene="puuD"
                     /locus_tag="OO544_001194"
     CDS             complement(4414..5178)
                     /gene="puuD"
                     /locus_tag="OO544_001194"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HCR6766837.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPGSPSNVQPHLYGENGDEPDADPGRDLLSMAIINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITACQHHIAEKQRL"
     gene            5390..6751
                     /gene="puuA"
                     /locus_tag="OO544_001195"
     CDS             5390..6751
                     /gene="puuA"
                     /locus_tag="OO544_001195"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HCR6766838.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRDAEGNLQPPC
                     APGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIPADGAVAEASPGQFEINLYH
                     TDNVLEACDDALALKRLVRLMAGKHKMHATFMAKPYEEHAGSGMHIHISMQNNRGENV
                     LSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRRFQPGMYVPTQASWGHNNRT
                     VALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILHGLDNELPLQEEVEGNGLEQ
                     EGLPFPIRQSDALGEFIENDHLRRYLGKRFCHVYHACKNDELLQFERLITETEIEWML
                     KNA"
     gene            6900..7064
                     /locus_tag="OO544_001196"
     CDS             6900..7064
                     /locus_tag="OO544_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6766839.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            7054..8439
                     /gene="puuP"
                     /locus_tag="OO544_001197"
     CDS             7054..8439
                     /gene="puuP"
                     /locus_tag="OO544_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HCR6766840.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            8573..8818
                     /locus_tag="OO544_001198"
     CDS             8573..8818
                     /locus_tag="OO544_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HCR6766841.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            9131..10774
                     /gene="sapA"
                     /locus_tag="OO544_001199"
     CDS             9131..10774
                     /gene="sapA"
                     /locus_tag="OO544_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein SapA"
                     /protein_id="HCR6766842.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            10771..11736
                     /gene="sapB"
                     /locus_tag="OO544_001200"
     CDS             10771..11736
                     /gene="sapB"
                     /locus_tag="OO544_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapB"
                     /protein_id="HCR6766843.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            11723..12613
                     /gene="sapC"
                     /locus_tag="OO544_001201"
     CDS             11723..12613
                     /gene="sapC"
                     /locus_tag="OO544_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HCR6766844.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            12613..13605
                     /gene="sapD"
                     /locus_tag="OO544_001202"
     CDS             12613..13605
                     /gene="sapD"
                     /locus_tag="OO544_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HCR6766845.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            13607..14413
                     /gene="sapF"
                     /locus_tag="OO544_001203"
     CDS             13607..14413
                     /gene="sapF"
                     /locus_tag="OO544_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HCR6766846.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            14481..14834
                     /locus_tag="OO544_001204"
     CDS             14481..14834
                     /locus_tag="OO544_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HCR6766847.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWNNEIDCNEEAV
                     PEIILQELNRRSPSP"
     gene            15202..15990
                     /gene="fabI"
                     /locus_tag="OO544_001205"
     CDS             15202..15990
                     /gene="fabI"
                     /locus_tag="OO544_001205"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HCR6766848.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            16135..17261
                     /locus_tag="OO544_001206"
                     /pseudo
     CDS             16135..17261
                     /locus_tag="OO544_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309887.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
     gene            17329..19263
                     /gene="rnb"
                     /locus_tag="OO544_001207"
     CDS             17329..19263
                     /gene="rnb"
                     /locus_tag="OO544_001207"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HCR6766849.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAKKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWQPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTGTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRGIIARPVA"
     gene            complement(19341..19444)
                     /locus_tag="OO544_001208"
                     /pseudo
     CDS             complement(19341..19444)
                     /locus_tag="OO544_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            19498..21488
                     /gene="pdeR"
                     /locus_tag="OO544_001209"
                     /pseudo
     CDS             19498..21488
                     /gene="pdeR"
                     /locus_tag="OO544_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
     gene            21635..21808
                     /locus_tag="OO544_001210"
     CDS             21635..21808
                     /locus_tag="OO544_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6766850.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            21898..22647
                     /locus_tag="OO544_001211"
     CDS             21898..22647
                     /locus_tag="OO544_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR6766851.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            22916..23134
                     /gene="osmB"
                     /locus_tag="OO544_001212"
     CDS             22916..23134
                     /gene="osmB"
                     /locus_tag="OO544_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HCR6766852.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(23260..23586)
                     /gene="yciH"
                     /locus_tag="OO544_001213"
     CDS             complement(23260..23586)
                     /gene="yciH"
                     /locus_tag="OO544_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HCR6766853.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGVVRIQRQTSGRKG
                     KGVCLITGVDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(23586..24323)
                     /gene="pyrF"
                     /locus_tag="OO544_001214"
     CDS             complement(23586..24323)
                     /gene="pyrF"
                     /locus_tag="OO544_001214"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HCR6766854.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLVDLGMTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALSAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(24516..25685)
                     /gene="lapB"
                     /locus_tag="OO544_001215"
     CDS             complement(24516..25685)
                     /gene="lapB"
                     /locus_tag="OO544_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HCR6766855.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(25692..26000)
                     /gene="lapA"
                     /locus_tag="OO544_001216"
     CDS             complement(25692..26000)
                     /gene="lapA"
                     /locus_tag="OO544_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HCR6766856.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGVAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            complement(26149..26913)
                     /gene="pgpB"
                     /locus_tag="OO544_001217"
     CDS             complement(26149..26913)
                     /gene="pgpB"
                     /locus_tag="OO544_001217"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HCR6766857.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFCIKAAFVLFAILAAVIIVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            27083..27673
                     /gene="ribA"
                     /locus_tag="OO544_001218"
     CDS             27083..27673
                     /gene="ribA"
                     /locus_tag="OO544_001218"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HCR6766858.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            complement(27737..30412)
                     /gene="acnA"
                     /locus_tag="OO544_001219"
     CDS             complement(27737..30412)
                     /gene="acnA"
                     /locus_tag="OO544_001219"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HCR6766859.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVEKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSTLELDMNDVEASLAGPKRPQDRVALPDVPKV
                     FAASNELEVNATHKDRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVILGLKRQPWVKASLAPGSKVVSDYLAKAKLTPFLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(30576..30671)
                     /locus_tag="OO544_001220"
     CDS             complement(30576..30671)
                     /locus_tag="OO544_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6766860.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            complement(30785..30952)
                     /locus_tag="OO544_001221"
     CDS             complement(30785..30952)
                     /locus_tag="OO544_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6766861.1"
                     /translation="MVGQEQLESSPLCQHSDNETETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            complement(30955..31119)
                     /locus_tag="OO544_001222"
     CDS             complement(30955..31119)
                     /locus_tag="OO544_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HCR6766862.1"
                     /translation="MLISDGDYMRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVV
                     VALLIWKVWG"
BASE COUNT         7329 a         8151 c         7930 g         7769 t
ORIGIN      
        1 cggcgcgggc gcgtccataa tattcgcgtt accgaccacg cccgaaagcg gcatcccgcc
       61 cgcgaggctt ttcgccatcg tcattaagtc cggcttatcg gcgtaatgat ccatggcaaa
      121 cagtttaccg gtacgcgcaa agccgctttg cacttcatcg gcaatcatca caataccgtg
      181 ctcgtcgcac aggcggcgaa tggcggcaac cagctctttt ggcgcaacgt tgaaaccgcc
      241 ctcgccctgc accggttcga aaataatggc cgcgacctgc ttcgcttcga tgtctgattt
      301 aaacaagcgt tcgatggcgt cgagggagtc ctatgttgaa atgccgtgta aatctgacgg
      361 ataaggtacg tgatacaccg aaccagggaa cgggccgaag ccgattttgt acggcgcaac
      421 ttttccggtc agcgccatgg tcatatatgt acgaccgtgg aagccaccgc taaacgcaat
      481 cacgccaggg cgtccggtat gggcgcgagc aattttcacc gcgttttcca ccgcttccgc
      541 accggtagtg aagaacgcgg ttttggcctg cccgctcacc ggggcaaggg cgttgatttt
      601 ctccgccagg gtgacgtagc tttcatacgg cacaatctga tacgcggtgt gggtaaactg
      661 ttgcagttgc tgctccaccg ccgcgaccag atcagggtgg cgatgtccgg tattcagcac
      721 cgcaatgcct gcggcgaaat cgatgtactc gttgccctca acatccttca gagtagcgtt
      781 ttcagccgac tgggcgaaga agttacacat cacgccaacc ccgcgtgggg tagcagaaag
      841 acgacgctga tggaattcat tgttgctcat gtttcacacc ttttgactct tcactgttaa
      901 tcggaaatta aacgcccaga cgatcgcgca ggctgtacca ggctgcgccc atcgcggtaa
      961 acggtactcg cagcgtgcgc ccgccgggga acgggtaata cggcagattg gcgaaggcat
     1021 cgaaacgttc ggcgtcgccg cgcagcagtt cggcaatcaa acgtccggcg agatgagtac
     1081 aggtgacgcc gtggccgctg taaccctgca tgtaatagat gttggtatca aggcgaccaa
     1141 actgcggcat gcgcgacagg gtcagcagga agttgcccgt ccagcggtag tcgattttga
     1201 cgcccttcag ttgcgggaag gttttcagca gtttcggcac aacaagacgc tcaacgtcat
     1261 ccgggtcgcg cgcgccgtag accacgccgc cgccgtacag caaacggttg tcggcggtaa
     1321 gacggtagta atcaagcaga taattacagt cttccacaca gtagtttttc gggatcagcg
     1381 aacgggctaa atcttccgac agccgttcag tggtgatcac ctgcgtgccg cacggcatgc
     1441 tgcgtttcgc cagttccggc tctactttat cgcccagata cgcattcccg gcgacaatca
     1501 catacttcgc cgtcacctga cctttggcag tgcgcaccac cgctggcgtg gtgtgctgga
     1561 tctgcgtcac ggcagaaagc tcatacacgc gcccgccgtt caggcggatg gcatcggctt
     1621 caccaatggc gaggtttagc gggtgaatat gcccaccgct gtgatccaac agtgcgccag
     1681 tgtagcgatc gctggcgacc tcgcggcgaa tggcgttagc gtccagcagc tccagctgtt
     1741 tattgccgta gcgttcccag ttctctttct gctcttcgag tgtggcgagc tgtttatcat
     1801 tcatcgccac aaacaggccg ccggggcggt agtcgcagtc aatctgatag cgtttgatac
     1861 gttcgcggat gatctcgcca ccctcgaaca tcatgctgcc gagcatacgg gcggtgtcca
     1921 tgccgtagct tttttcgatc acgtcgatgt cgcggctgta ggagttcaca agctgcccgc
     1981 cattgcgccc gcttgcgcca aagccgatgc gtgaggcttc gagaaccact acgtcaaagc
     2041 ccgcttccgc cagatgcagc gcggaggaga gcccggtata gccgccgcca accacgcaaa
     2101 cgtcgcaggt gattgactca ttcagtgtgt cgaatggtgc atatttattc gcactggcgg
     2161 cgtagtaact gctggtatgt tcggtcattt caggcctcca ggcttatcca gatggttttc
     2221 agttcagtga atttttcaag ggcatgcagg gatttgtcgc gaccgttgcc gctctgctta
     2281 tagccgccaa acggcacggt catatcgccg tcgttgtagt tattgacgaa gacggaaccg
     2341 gctttcaggc ggcggctcat acggtgcgcg cgggagaggt cgcgcgtcca taccgccgcg
     2401 ccaaggccgt actggctgtc gttggcaagc tgtagcgcct ggtcttctga tgtgaaacgc
     2461 gtgaccacca gcaccggacc gaaaatctct tcgcggctta aggacgcatt cgggtcaaca
     2521 tccacaaaga tggtcgggcc gatggcggca gccagctcag cgttacgacc atccagcagc
     2581 agttgccctt tgctttcgcc ttcacgaata aagctatgga ccgagtcggc gtgggcgcaa
     2641 tcgattaagg tgcccatggt ggttacggta tcaagtggat ggcccggctg ccagttttgc
     2701 gcctgctgtt ttaacagggc taagaattca tcggcgatgc tctcttccag taacaaacgc
     2761 gttccggcga tgcacacctg gccctggttg tagaagatgc ctgcggcggt ggcgcttgcc
     2821 gctttttgca gatccggaca atcggcgaaa acgatgttgg cgcttttgcc gcccgcttcc
     2881 agccagacgc gtttcatgtt gctatcaccc gcgtctttca gcagttgttt acccgtacgc
     2941 gtggagccgg taaaggcgat ggcatcgata tcgttatgac gcgaaagcgc ctgcccggct
     3001 tcatgaccat aaccgctcac tacgttcagc acgccatccg gcagccccgc ttcttctttt
     3061 gccagcccgg cgagacgaat cgcactgagt ggtgattttt cagacggttt tagaatcacg
     3121 ctgtttcccg ccgccagcgc cgggccgagt ttccagcaag tcagcagcag cgggaagttc
     3181 cacggcacga tggcggcaat cacgccgacc ggttcacgca cgatcatcgc cagctcatgg
     3241 ctactggtgg tcgccacttc gccatacact ttgtcgatcg cttcggcgta ccagcgaatg
     3301 gcgcgcgccg cgccgggaat atcatcacgc agactgtgac gaatcggttt gccggtgtcg
     3361 agagtttcca gcagtgccag ctcttcggcg tgggcttcca ttaaatcggc gagtttattc
     3421 agtaccgctt tacgtttagc cggagaagag agtgaccagt cgccgcgttc aaatacgccg
     3481 cgtgctgcgc tcaccgcatg gtcgatatcg acgctcttgc cgcgggcaat tttcgccagc
     3541 ggtgcctagg tgaccggatc aacggtttca aaggtttcat tttccgccgc agcagtatat
     3601 tcaccgttaa taaataagcg gttttcaatg gcgagactta acgctttatc ctgccagtaa
     3661 gccagatgat gaaaattcat tatgactcct gtttcacgtc tatcagatat atgcagtttg
     3721 tgaacgtcag ggcgcagaga tccccgggcc gcatgcccgt ttatttctgg cgacattctt
     3781 tatggattgc cgggcgcatt gcgcttaccc ggcttacaga acaaaaagat taaaacgtgg
     3841 tgggcgtatg ggcgctgata attcggcaaa tacctgccga cgtattactg aaactgtgcg
     3901 ggatgccggt attaatggca taactttgcc ccgcgacgag gtggtaatcc tgaccattaa
     3961 tcgtcagaac aatttcacct tccagtactg tgcctatttc ctcaccctga tgcttaattc
     4021 tttccccagt ggttgtgccc ggctggtacg tttcaaagat catcgccagc gtgcgattcg
     4081 ggttaccgtt atgaaccagc ttcattgaca caccctgact gcccatctca attaagtcgt
     4141 cctgattaat gacgacctgc ggctcatcag gtttttccgg ctcggaaaag aattccgaga
     4201 gtgacagacc atacaccttc agcagctttt gcagcgtact gatggcaggg ctgactttat
     4261 cttgttctat cgtactgata gcactgtgag tcagcccgga gagttcggcg gcacgacgtt
     4321 gtgaaagccc ctgctgctgg cggatttccg acaagcgttt tcctggcgcc agtccctcat
     4381 cactcatatt tgcatttcct taaactgtag tggtcagagt cgctgttttt cagcgatatg
     4441 gtgctgacaa gcggtgataa agccctcgaa caatatacgc gaaagcgcgt actcgctact
     4501 gttccattcc gggtgccact gtacgcccag cgcaaaagga tgattgatga cgctaaccgc
     4561 ctcaactaag ccatccggcg agcgagcttc aacacgcaac cgtgggctaa cgaccttcgc
     4621 cccttgtcca tgtagagagt ttacccaaaa gttgctacat tcaggtaaca acgcagacag
     4681 taatcccccc tcttcaacct gaacttcatg cgacggtgca tattgctgtt ccaccggcag
     4741 ttcgggatct tcccgatgtt ccagcaattc aggctgttcg cacagcttgc gatgcaacga
     4801 accaccggtt gccaccacca gttcttgtaa accccggcag atggcgaaaa tggggatgcg
     4861 cctttcgagt gcggcattaa ttatcgccat gctcagaaga tcacgcccgg gatcggcgtc
     4921 aggctcatcg ccgttttcac catataggtg cggctgcaca ttgctgggac taccaggaag
     4981 ataaatgcca tcgagtttcg gcaaaagttg ttcaagtaac gacggttccg ccagcgcatg
     5041 tggtagcgca attggcaagc cgcctgcgtg gatgatggca ttcaggtact tttcttgcag
     5101 agtctgggtc gcatgaccct taagcctgtt cctgcacatt acgacaccga taaccggatt
     5161 gttcattata ttttccatgc tcaatctcac aaagtggact aaattatcgc cattactgcg
     5221 ctttaaatga cctttatgtt caatattttt tcaatctagc agtgggtttt catttttgca
     5281 aactcaattt aacatttgac aaacatttag tttgcataca gattcgaatg gtggtcatta
     5341 tattttacgc tttgataacg agcggaaaac aaaccaaagg cgaagaatca tggaaaccaa
     5401 tatcgttgaa gtagagaact ttgttcagca gtcagaagag aggcggggta gcgcctttac
     5461 gcaggaagtg aaacgctacc tggagcgcta cccgaatacg caatatgttg atgttttact
     5521 tactgatttg aatggttgct tccgtggaaa acgtatccct gtttcaagcc tgaagaagct
     5581 cgagaaaggg tgttatttcc cggcctcggt atttgcaatg gatattctgg gcaacgtagt
     5641 agaagaagca ggtctgggtc aggaaatggg cgagccggat cgtacctgtg ttcctgttct
     5701 cggttcctta actccttctg ccgccgatcc agagtttatc ggtcagatgc tcctgaccat
     5761 ggtcgatgaa gatggcgctc cctttgacgt tgagccgcgg aacgttctta accgcctgtg
     5821 gcagcagctg cgccagcgcg acgctgaagg gaatctgcaa ccgccctgcg cgcctggcac
     5881 cgatgaccgc aatacacaaa gccaggttta ctccgttgat aacctcaacc acttcgccga
     5941 cgtgctcaat gatattgatg aactggcgca gttgcagctg attccggcag atggcgcggt
     6001 cgctgaggcc tcgccgggtc agtttgaaat caacctttac catactgata acgtgctgga
     6061 agcctgcgat gatgcgctgg cactaaaacg cctggtgcgt ctgatggcag gaaagcataa
     6121 gatgcacgcc acttttatgg cgaagccgta tgaagagcac gcgggcagcg ggatgcatat
     6181 ccatatcagt atgcaaaata atcgtggcga gaatgtgctt tctgacacag aaggcgaaga
     6241 ttcgccgctg ctgaaaaaga tgctcgccgg gatgattgac ctgatgccgt cgtcgatggc
     6301 gttgctggca ccaaacgtga actcgtatcg ccgcttccag ccgggaatgt atgtgccgac
     6361 gcaggcgtcg tggggccata acaaccgcac cgtcgccctg cgtattccgt gcggcgaccg
     6421 tcataatcac cgcgtggaat atcgcgtggc gggtgccgat gccaacccat atctggtgat
     6481 ggcagcgatt tttgccggta ttttgcatgg ccttgataac gagctgccgt tgcaggaaga
     6541 agtcgaaggc aacgggctgg aacaggaagg cttacccttc ccgattcgcc agagcgatgc
     6601 cctgggtgag tttatcgaga atgatcacct gcgccgctat ttaggcaaac ggttttgcca
     6661 tgtgtatcac gcctgtaaaa atgatgagct gttgcagttt gagcgcctca tcacagaaac
     6721 agaaattgag tggatgttga aaaacgcgta attccccttg cggggttgtt tcatcggcct
     6781 gagcggagcc aggcctgaca tttgctggta atccagacat ttcccggatg tcgcgttatg
     6841 gagcgcaggc ggcaacgggc ttccgttaac gattaagact gtgacgagga aatgagatga
     6901 tgccgatgat taaatccccg catggcgagg gaggctgcgt atgcgcccct cctgctactg
     6961 actggactcc gccccctttg cttcccttgc taaacaggtt tgatttcagg tcgacacgac
     7021 cgcaaacctt attacgcagg ggaggcagca attatggcta ttaattcacc actgaatatt
     7081 gctgcgcaac ccggcaaaac ccgtctgcga aaatcactga aattgtggca ggtggtaatg
     7141 atgggtctgg cctatctcac gccgatgacc gtatttgata cttttggtat tgtctccggc
     7201 attagcgacg gtcacgttcc ggcgtcctat ttgctggcgc tggcgggcgt gctgtttacc
     7261 gctatcagct acggcaaact ggttcgccag tttccggagg ccggttcggc ctatacctac
     7321 gcgcaaaagt cgattaaccc gcacgtcgga tttatggtcg gctggtcatc actgctggat
     7381 tatctctttt tgccaatgat caacgttttg ttggcgaaaa tctatctctc cgccctcttc
     7441 ccggaagtgc cgccgtgggt gtgggtggta accttcgtcg ccattttaac cgccgcgaat
     7501 ctgaagagcg tcaacctggt cgctaacttc aataccctgt ttgtactggt gcaaatctcc
     7561 atcatggtgg tgtttatctt cctggtggtt cagggactgc ataaaggaga aggcgttggc
     7621 accgtctggt cacttcagcc gtttatcagc gagaacgcgc acctgatccc gattattacc
     7681 ggtgcgacga ttgtctgttt ctcgttcctc ggtttcgatg cggtgaccac gctttcggaa
     7741 gagacgccgg acgccgcacg cgtgatcccg aaagccatct tcctgacggc ggtatacggc
     7801 ggcgttatct ttatcgcggc gtcgttcttt atgcagctgt tctttcccga tatcagccgc
     7861 tttaaagacc cggacgccgc actgcctgaa attgcgctgt acgtcggcgg caagctcttt
     7921 cagtcgattt tcctctgcac cacgtttgtg aacacattag cgtctggcct ggcttcacat
     7981 gccagcgtgt cgcgtctgct gtatgtgatg gggcgtgaca atgtgtttcc ggagcgcgtg
     8041 tttggctatg tgcacccaaa atggcggact ccggcactca acgtcattat ggtcgggatt
     8101 gtcgcgctgt cggcgctgtt ctttgattta gtcaccgcga cagcactgat taacttcggt
     8161 gctctggtgg cgtttacctt cgtgaatctg tcggtgttta atcatttctg gcggcgtaaa
     8221 ggaatgaata aaagctggaa ggatcacttc cactatttgc tgatgccgct ggttggcgcg
     8281 ctgacggtgg gtgtgctgtg ggttaaccta gagtcaacgt cactgacact cggtctggta
     8341 tgggcttcgt taggcggcgc atatttgtgg tatctgatcc gccgctatcg caaagtgccg
     8401 ctgtacgaag gtgacagaac gccggtgagc gaaacgtaat agccggatgc gcggcgcatt
     8461 atcctgcctg agggtaacgc gccgcgccat taactataaa aagatgtata acccggatca
     8521 cgagtgctat catccgccct tatgcttagc gcattaaccc caggggcaaa taatgaacca
     8581 cgatatcccg ctaaaatatt ttgatattgc cgatgagtac gcgaccgaat gcgcagagcc
     8641 tgtcgcagag gctgaacgca caccgctggc ccactacttc cagttactgc tcacccgttt
     8701 gatgaacaat gaagagatca gcgaagaagc ccaacatgaa atggctgctg aagcggggat
     8761 taatcccgta cgcattgatg agatcgcaga gtttttgaat caatggggta atgagtaatt
     8821 atttaattgc agacgatcgg tcagtatcgt tgtccggata aagcgttcac gccgcatccg
     8881 gctaacaatg acacgcactg cagcgtgctt taatgggttt ctcacgatcg taccgctctg
     8941 ctcctcgcta agttgttctg tgagttatag ttatagagaa atcacgggta cgaatgttga
     9001 acagatggcg acgccacctg ttcaacgacc aaccacacgg ccctcattac gacccctgaa
     9061 cacaaacggt aatggcaaat gtcattaaat attagtaaga tagcctcttt acataaaatc
     9121 ccttaatatt atgcgccagg tattatcgtc tcttttggtg attgctggac ttgtgagtgg
     9181 tcaggcaatc gccgcgcctg aatccccccc gcatgctgat atccgcgaca gcggttttgt
     9241 ctattgcgtc agcgggcaag tcaacacctt taacccatcc aaagcgagca gtgggttaat
     9301 tgtcgatacc cttgccgccc agttttatga tcgactgctg gatgtcgatc cctataccta
     9361 tcgcctgatg ccggaacttg ccgaaagctg ggaagtactc gacaacggcg cgacctatcg
     9421 cttccacctg cgtcgcgatg ttccgtttca aaaaaccgac tggtttactc ccactcgtaa
     9481 aatgaatgcc gacgatgtgg tgtttacctt ccagcgaatt tttgaccgca acaacccgtg
     9541 gcataacgtc aacggcagca acttccccta cttcgacagc ctgcaatttg ccgataacgt
     9601 gaaaagcgtc cgcaaactgg ataatcatac cgttgagttc cgactcgccc agccggatgc
     9661 ttcttttttg tggcacctcg caacccatta tgcttcggtc atgtcggcag aatatgcccg
     9721 gaagttagag aaagaagatc gccaggagca actcgaccgt caaccggtcg gcaccggtcc
     9781 gtatcagttg tcggaatacc gcgccgggca atttattcgc ctacaacgtc atgatgactt
     9841 ctggcgcggt aaaccgttaa tgccgcaggt agtggtggat ttaggctccg gcggcaccgg
     9901 acgtctgtcg aaactcctga ccggggaatg cgacgttctg gcctggcctg ctgccagcca
     9961 gctatccatt ttgcgtgacg acccgcgctt gcgtttaacg ctgcgtcctg ggatgaacgt
    10021 cgcctatctg gcatttaaca ccgccaaacc gccgctaaat aatcccgctg tccgccatgc
    10081 actggcactg gcgattaata accagcgcct gatgcaatcc atctattatg gtacggctga
    10141 aacggcggcc tctattttac cgcgcgcctc gtgggcctat gacaacgagg ctaaaattac
    10201 tgaatacaat ccggcgaaat cgcgcgaaca gttgaaggcg ttggggctgg aaaacttaac
    10261 gctgaaactg tgggtgccca cacgttcgca ggcgtggaac cccagtccac tgaaaactgc
    10321 cgaactgatt caggcggata tggcgcaggt tggcgtaaaa gtggtgattg tgccggtaga
    10381 aggtcgcttt caggaggcgc ggttgatgga tatgagccat gatctgacat tatccggttg
    10441 ggcgacggac agtaacgacc cggacagttt cttccgtcct ttactgagct gcgcggcaat
    10501 tcattcacag accaacctcg cccactggtg cgatccgaaa ttcgacagcg tgttgcgtaa
    10561 ggcgctctcc tcgcagcagc tggcggcgcg tattgaagcc tatgacgaag cgcagagtat
    10621 tctggcgcag gaattaccta ttctgccgct ggcgtcgtca ttgcgtttgc aggcctatcg
    10681 gtacgatatc aaaggtctgg tacttagccc gtttggtaac gcctcctttg ctggggtgta
    10741 tcgcgagaaa caggatgagg tgaaaaaacc atgattatct tcaccttacg ccgcattttg
    10801 ttattgattg tcaccttgtt cctgctgacc ttcgttggct tcagcttgag ctatttcacc
    10861 cctcatgcgc cgttacaagg cgcgtcactg tggaatgcct gggtgttctg gtttaacggc
    10921 ctgatccact gggattttgg tgtttccagc attaacggtc aaccgattgc cgaacagtta
    10981 aaagaggtct tcccggcgac gatggaactg tgcatcctcg ccttcggttt tgcgctgatt
    11041 gttggcatcc ccgtggggat gattgccggg attacgcgcc ataagtggca ggataacttg
    11101 atcaacgcca tcgccttact gggcttttca atcccggtgt tctggctggc gcttctgttg
    11161 acgctgtttt gttcactcac gctaggttgg ttgcccgttt cagggcgttt cgatctgctc
    11221 tacgaagtga aaccgattac cggttttgcg ttgattgatg cctggctttc ggactcacca
    11281 tggcgggatg aaatgatcat gagcgcaatc cgccatatga tattgcccgt gatcaccctg
    11341 tcggttgcgc caacaactga agtgatccgc ctgatgcgta tcagcactat cgaagtgtat
    11401 gaccagaact atgtaaaagc ggcggcgacc cgcggtttgt cgcgctttac tattttgcgt
    11461 cgccacgttc tgcataacgc gttaccgccg gttattcctc gcctgggctt acagttttcc
    11521 accatgctga cgctggcgat gatcaccgaa atggttttta gctggccggg tttagggcgc
    11581 tggttaatta acgccatccg ccagcaggat tacgcagcca tttccgccgg agtgatggtg
    11641 tgtggctcac tggtcattat tgttaacgtg atttctgata ttttgggtgc catggctaac
    11701 cctctgaaac ataaggaatg gtatgcctta cgatagcgta tacagcgaaa agcgcccgcc
    11761 gggcacgctg cgtaccgcct ggcgcaaatt ttatagtgac gcctctgcga tggtcggcct
    11821 gtacggctgc gcggggctgg ctgtactgtg tatttttggc ggctggtttg cgccttacgg
    11881 catcgaccag caatttctcg gttatcaatt attgccgcca tcatggtcgc gctatggcga
    11941 agtttctttc ttcctgggga ctgacgacct cggacgcgat gttttaagcc gtttactgag
    12001 cggagctgcg ccaaccgtgg gtggcgcatt tgtggtgacg cttgccgcga cgatctgtgg
    12061 cctggtactg ggcaccttcg ccggggcgac acacggcctc cgctcagcgg tgcttaacca
    12121 tattctggat accttactgg cgatcccttc gctgctgttg gcaattatcg ttgtagcgtt
    12181 tgccggaccg agtttgtccc acgctatgtt tgctgtctgg ctggcattgc tgccgcgtat
    12241 ggtacgttcg atttacagca tggtgcatga cgaactggaa aaagagtacg ttatcgccgc
    12301 ccgtctggat ggcgcatcaa cgctgaatat tctctggttt gctgtgatgc caaacatcac
    12361 cgccgggctg gtcaccgaga tcacccgcgc actgtcgatg gcaattctcg atatcgccgc
    12421 gctgggcttt ctcgatctcg gcgcacaact cccctcgcct gaatggggag cgatgctcgg
    12481 tgatgcgctg gaactgattt atgtcgcccc gtggactgtc atgctgccag gtgcggcaat
    12541 tatgattagc gtgttgttgg ttaacctcct tggcgatggc gttcgtcgtg cgattattgc
    12601 gggggtggaa taatgccatt actcgatatt cgtaacctga ccattgaatt taaaaccggt
    12661 gatgagtggg ttaaagccgt cgaccgggta agtatgacct taaccgaagg tgaaatccgc
    12721 ggtcttgttg gtgaatccgg ttccggcaag agtttgattg cgaaagcgat ttgtggggtg
    12781 aataaagata actggcgtgt tactgctgac cgtatgcgtt ttgatgatat cgatttgctg
    12841 cgtctctccg cacgcgaacg gcgcaaactg gttggccata acgtgtcgat gattttccag
    12901 gaaccgcagt cgtgtcttga cccttcagaa cgtgtgggtc gccagttgat gcaaaacatc
    12961 ccagcctgga cctataaagg ccgttggtgg cagcgttttg gctggcgcaa acgccgtgcc
    13021 attgaactgc tgcaccgcgt ggggattaaa gatcacaaag atgcgatgcg cagttttccc
    13081 tatgagttga ccgaaggtga atgtcagaaa gtgatgatag ccattgcact ggcgaatcaa
    13141 ccgcgtctgc tgattgctga cgaaccgacc aactcaatgg agccaacaac ccaggcgcaa
    13201 atctttcgcc tgctgacgcg tctcaaccaa aacagcaata ccactatttt gcttatcagc
    13261 catgacttac aaatgcttag ccaatgggcg gataaaatta acgtgcttta ctgcggtcaa
    13321 acagtggaaa ccgcgccgag taaggagttg gtgacgatgc cacatcatcc ttatacccag
    13381 gcgctgatcc gcgcgatacc agacttcggc agcgcgatgc cgcataaaag tcgcctcaat
    13441 acgctgcccg gcgctatccc actgctggaa cagttaccga ttggttgtcg tctggggcca
    13501 cgttgcccgt atgcacaacg agaatgcatt gtgacgccac gtttgacggg ggcgaaaaat
    13561 catctctatg cctgtcattt cccgctgaac atggagaaag agtgagatga tcgaaacgct
    13621 gcttgaagtg cgtaatttaa gtaaaacgtt ccgctaccgg accggctggt ttcgtcgtca
    13681 gaccgtagaa gcggtaaaac ccttgagctt tacgctacgt gaaggccaga cactggcgat
    13741 tattggcgag aatggttcgg gtaaatccac gctggcaaaa atgctggcgg gaatgataga
    13801 gcctaccagc ggcgagttat tgattgacga tcatccactg cattttggcg attattcctt
    13861 ccgcagtcag cgtattcgca tgatttttca ggatccttcg acctcgttga atccccgtca
    13921 acgtatctcg caaattctgg attttccact gcgcctgaac accgacctgg aaccggaaca
    13981 gcgtcgcaaa cagattattg aaacgatgcg tatggttggg ctgctaccgg atcacgtcag
    14041 ttactatccg catatgttgg cacccggaca aaaacagcgt ctgggtctgg cgcgcgcgct
    14101 aatattgcgc ccaaaagtca ttattgccga tgaagccctc gcttcactgg atatgtcgat
    14161 gcgttcgcag ttgattaatc tgatgctgga gttacaggaa aaacagggca tttcgtatat
    14221 ttatgttacc cagcatatcg gaatgatgaa gcacattagc gaccaggtgc tggtgatgca
    14281 tcagggcgag gttgtcgagc gaggcagcac cgcggatgtg ctggcatcgc cgctgcatga
    14341 actcaccaaa cggctgattg ccgggcactt tggcgaagca ttgacagcgg atgcgtggcg
    14401 taaagatcgc taattttctg aatcctcctt ccagattatc cacctcattc tgacaagaga
    14461 ttaaatcttg tcagaatgag gtgatggata aaataaaatc aaatgcgcgt gatttaagac
    14521 gcaatctcac tttgcaggaa cgaaagctct ggcgatatct tcgcagccga cgttttggtg
    14581 atttcaaatt tcgccgtcaa catccggtgg ggagctacat tctcgatttt gcttgctgct
    14641 cggcgcgcgt agtcgttgag ctggatggtg ggcagcatga tttggcagtt gcctatgata
    14701 ccaggcgcac tagctggctt gagtcgcagg gctggaccgt gctgcgtttc tggaataacg
    14761 agattgattg taatgaggag gcggtgccgg agattattct gcaggaactg aaccgccgat
    14821 caccctcacc ctgaaagggc gagggggccg accgagccga atagctgttg tggtgaaaac
    14881 atggagacag tgctggagaa tattcggcaa ggtctgaacc atcccagcca tcgccataaa
    14941 agggttaggg gctgtatgag cctgtttgtt gctggggtaa caatatttgc acaatatggt
    15001 cccctcgccc ctctggggag agggttaggg taaggggaaa agcgcccacc ctgccgcagc
    15061 ctgctccggt cggacctggc aactatagct actcacagcc aggttgatta taataaccgt
    15121 ttatctgttc gtactgttta ctaaaacgac gaatcgcctg attttcaggc acaacaagca
    15181 tcaacaataa ggattaaagc tatgggtttt ctttccggta agcgcattct ggtaaccggt
    15241 gttgccagca aactatccat cgcctacggt atcgctcagg cgatgcaccg cgaaggagct
    15301 gaactggcat tcacctacca gaacgacaaa ctgaaaggcc gcgtagaaga atttgccgct
    15361 caattgggtt ctgacatcgt tctgcagtgc gatgttgcag aagatgccag catcgacacc
    15421 atgttcgctg aactggggaa agtttggccg aaatttgacg gtttcgtaca ctctattggt
    15481 tttgcacctg gcgatcagct ggatggtgac tatgttaacg ccgttacccg tgaaggcttc
    15541 aaaattgccc acgacatcag ctcctacagc ttcgttgcaa tggcaaaagc ttgccgctcc
    15601 atgctgaatc cgggttctgc cctgctgacc ctttcctacc ttggcgctga gcgcgctatc
    15661 ccgaactaca acgttatggg tctggcaaaa gcgtctctgg aagcgaacgt gcgctatatg
    15721 gcgaacgcga tgggtccgga aggtgtgcgt gttaacgcca tctctgctgg tccgatccgt
    15781 actctggcgg cttccggtat caaagacttc cgcaaaatgc tggctcattg cgaagccgtt
    15841 accccgattc gccgtaccgt tactattgaa gatgtgggta actccgcggc attcctgtgc
    15901 tccgatctct ctgccggtat ctccggtgaa gtggtccacg ttgacggcgg tttcagcatc
    15961 gctgcaatga acgaactcga attgaaataa tcattctgtt ggtaaatatt gggcggcgtt
    16021 ctgccgcccg ttatctctgt tatacctttc tgatatttgt tatcgccgat ccgtctttct
    16081 ccccttcccg ccttgcgtca ggataacgat ttcctttacg accaaggagc gcccatggaa
    16141 caacgccaca tcaccggcaa aagccactgg tatcatgaaa cgcaatccag tactgcggag
    16201 tatgacgttc tgcctctggt cccggaagcc gcaaaggtca gcgatccctt tctgctcgac
    16261 gtgatccttg atgaagaaac gctggctccc ttcctttcat ggctggtccc tgcgcgcgtt
    16321 cttgcagtgg aattgttccc tgaccagctt accgtgaccc gttcacagac cttcaccgct
    16381 tatgaacgct tgtctacggc cctgacggtt gctcaggttt gcggcgtcca gcggttatgt
    16441 aactactatt cggcgcgact tacgccgctc cccgggcctg attcctccag ggaaagcaat
    16501 cataggttgg cacaaatcac gcaatatgcc cgccaactgg ctagctcgcc ttctattatc
    16561 gacaaccgat cgcgccagca tctgaatgac gtcggtctta ctgcctggga ctgtgtgatc
    16621 attaaccaaa tcattggttt tattggcttt caggcgcgga ccattgcgac atttcaggct
    16681 tatctcgggc acccggtacg ctggttaccc ggtctggaaa tacaaaacta cgccgacgcg
    16741 tcactgtttg ctgatgaatc aatacgctgg cgaagcagct atgaggtgga aaaactacct
    16801 gaagagtaca caaaaagttc aacagcagaa ctttgccaac tggccgaaac actctctctc
    16861 caccctattt cactttccct tctcgaaagg ttgttaaaca gtacacgggt taatacacag
    16921 ccggataata agcttgcggc gttgttatgc gcgcggataa atggcagtcc tgcttgtttt
    16981 gccgcctgta tggattcatc aaatgaatat aaaaaatcag cccccttctg cgcaagggcg
    17041 aaaatgaaat taaccaatgg gctgaccgtc attctgttga gcacgctacc gttcaggcga
    17101 tacaatggct gacccgagca cccgatcgct ttagcgccgc ccagttcagc cctttactcg
    17161 gacacgaaaa atcatcaacg cagattatta atctgctggt atggagcggg ctgtgtggct
    17221 ggataaatcg cttaaaaatc gcgttgggtg agacatatta accttgccgc gtcagacaga
    17281 ttcgcgtaaa actgtcagcc gctctaatgg ccaccaaaat agacaattat gtttcaggac
    17341 aacccgctgc tagcgcagct taaacagcaa ctgcattccc agacgccacg cgctgaaggg
    17401 gtggtaaaag ccacagaaaa aggctttggc ttcctggaag tcgacgcgaa aaaaagttat
    17461 ttcattccgc cgccgcagat gaaaaaagtc atgcatggcg accgaattat cgcggtgatc
    17521 cacagtgaaa aagaacgtga atccgcagag ccagaagaac tggttgaacc gttcctgact
    17581 cgtttcgtgg gtaaagttca gggcaaaaat gaccgtctgg ccatcgttcc tgatcatcca
    17641 ctcttaaaag acgccattcc ttgccgcgca gcccgtggcc tgaaccacga gtttaaagaa
    17701 ggcgactggg cggttgccga aatgcgccgt catccgctga aaggcgatcg ttctttctat
    17761 gctgaactga cacaatacat cacttttggt gacgaccact ttgtaccgtg gtgggttacc
    17821 cttgcgcgcc ataatctgga aaaagaagca ccagacggcg tcgctaccga aatgctcgat
    17881 gaaggtctgg ttcgtgaaga tctgaccgcg ctggattttg tcaccatcga cagtgccagc
    17941 acagaagata tggatgacgc ccttttcgct aaggcgttgc cggatgacaa acttcagctg
    18001 attgtggcga ttgccgatcc aaccgcgtgg attgctgaag gcagcaagct ggacaaagcc
    18061 gcgaaaattc gcgcattcac caactatctg cctggcttca acatccctat gcttcctcgc
    18121 gagctttctg acgatctctg ctcactgcgc gccaatgaag tccgcccggt actggcatgc
    18181 cgcatgacgc tctccgctga tggcaccatt gaagataata tcgaattctt tgccgccacc
    18241 atcgaatcca aagcgaagct ggtgtatgac caggtttctg actggctgga aaataccggt
    18301 gactggcagc ctgaaagtga agcaattgcc gaacaagtcc gtttgctagc gcaaatttgc
    18361 caacgccgcg gcgagtggcg tcataaccac gcactggtgt ttaaagatcg cccggattac
    18421 cgctttattc tcggtgaaaa aggtgaagtg ctggatatcg tcgccgagcc tcgtcgcatt
    18481 gccaaccgta tcgtcgaaga agcgatgatt gccgctaaca tttgtgcggc ccgcgttctg
    18541 cgcgataagc tcggttttgg catctataac gtgcatatgg gctttgatcc ggcgaatgcc
    18601 gacgcgctgg cagcgttgct gaaaacgcac ggtctgcatg tcgatgccga agaagtgctc
    18661 acgctggacg gtttctgcaa actgcgtcgt gaactggatg cgcaaccaac tggtttcctc
    18721 gacagccgca ttcgtcgctt ccagtcattt gctgaaatta gcactgaacc cggtcctcac
    18781 tttggcctcg gtctggaagc atacgccacc tggacttcgc cgatccgtaa atatggcgac
    18841 atgatcaacc accgtctgct gaaagcggtt atcaaaggcg aaactgcgac gcgtccgcag
    18901 gatgaaatca ctgtccaaat ggccgaacgt cgccgtctca accggatggc agaacgtgat
    18961 gttggcgact ggttatacgc acgcttcctg aaagataaag ccgggaccgg cacccgtttc
    19021 gcggcggaaa ttgtcgatat cagccgtggc ggcatgcgtg tgcgtttggt tgataacggc
    19081 gctatcgcct ttattccggc acctttctta cacgctgtgc gcgatgaact ggtttgcagc
    19141 caggaaaacg gcactgtaca aattaaaggt gaaacggttt acaaagtaac tgacgttatt
    19201 gacgtcacca ttgccgaagt ccgcatggaa acccgcggca ttattgcgcg cccggtcgcg
    19261 taatctcctt tcacggccca ttcctcatgg atgggccgtt tatttccccg ctctaccttc
    19321 gttatatcca ggcagatttt ttaactgact ttcgtttgaa aactggcgtt tttccaaaac
    19381 tggattacaa ataaatatat aaaaaaacaa tgcgtttagc cgccgttaat ttcatgtaag
    19441 tcatgttgtt ccttttattt aaccgcggac tccgcagtta accggaggat atgcatcatg
    19501 aaaaccgtta gggagtccac aacgttgtac aactttctcg gatcgcacaa tccatactgg
    19561 cggttgacgg aaagcagcga tgttttgcgc ttttttacca ccgaaaccac agaacctgat
    19621 cgtactttgc agttatctgc cgaacaggct gctcgcatca gggaaatgac ggtcatcacc
    19681 tccagcctga tgatgagtct gaccgtcgat gagagcgatc tttctgtgca tctggtagga
    19741 cgaaaaatca ataaacggga atgggctggc aacgcgtctg catggcatga cacaccggca
    19801 gttgctcgtg atttatcaca cgggctttcc tttgctgagc aggtagtttc tgaagcacat
    19861 tccgcaatag tgattctcga cagccggggg aatatccaac gcttcaatcg gttatgtgaa
    19921 gattacactg ggttgaaaga acacgacgtc attggccaaa gcgtgtttaa actgtttatg
    19981 agccgtcgtg aagctgcggc atccaggcgc aataaccgtg tattttttcg aagcggcaat
    20041 gcatatgaag tcgaactgtg gataccaaca cgtaaaggcc agcggctgtt tctgtttcgc
    20101 aataaaataa atttgtccac agcggcagtg gcaaaaacga gattttttta atctgttccg
    20161 gtaccgacat taccgaagag cgccgcgctc aggagcgact gcgtattctg gcaaataccg
    20221 acagtatcac cggactgccg aatcgtaacg caatgcagga gttaatcgat cacgctatta
    20281 atcaggctga taacaataaa gttggggttg tgtatcttga tttggataat tttaaaaagg
    20341 tcaacgacgc ctatgggcat ttgtttggtg accagttatt acgcgacgtg tcattggcta
    20401 ttttaagctg tctcgaacat gaccaggtgt tggcgcgtcc aggtggcgat gagtttctgg
    20461 tactggcatc caatacctca caaagcgcgc tggaagcaat ggcatcacga attttgaccc
    20521 gcttacgcct cccctttcgt attggtttaa ttgaagttta taccagctgt tcagtaggta
    20581 tttcgctctc tcccgaacat ggttcggaca gcgcgactat tattcgtcac gccgacacag
    20641 caatgtacac agcgaaggaa ggcggacgag gacaattttg cgtttttact ccagaaatga
    20701 atcagcgggt atttgaatat ctctggctgg ataccaactt gcgtaaagca ctggaaaacg
    20761 atcagttggt tattcactat caaccgaaaa tcacctggcg tggcgaagtg cgcagtctgg
    20821 aagcactagt acgttggcag tcacctgaac gtgggttgat tccaccgttg gacttcattt
    20881 cctacgccga agagtcaggg ctaattgtgc ctttaggccg ttgggtgatt ctcgatgtcg
    20941 tacgccaggt ggcaaagtgg cgggataaag gcataaacct gcgagtggcg gtaaatattt
    21001 ctgcacgtca gctcgccgat caaaccattt tcaccgccct gaaacaggtt ctccaggaac
    21061 tcaattttga atactgccct atagatgttg aactgacaga gagttgtctg attgagaatg
    21121 atgaactggc actgtctgtt attcaacaat ttagccaact aggtgcgcaa gtgcatctgg
    21181 atgattttgg taccggctac tcttcacttt cgcaactggc gcgctttccg atcgatgcca
    21241 tcaaacttga ccaggttttt gttcgagata ttcacaaaca acctgtctcg cagtcactgg
    21301 tccgggcgat cgtcgctgtg gcccaggcat tgaatcttca ggtgatcgcc gaaggtgtag
    21361 agagtgcaaa ggaagatgct tttttaacca agaacgggat caatgagcgg caaggatttt
    21421 tgtttgccaa accgatgccc gccgtcgcct tcgaacgctg gtataaacgc tatctgaagc
    21481 gcgcataagt agatgaatat atatacttag cgctatatcc cgtcattata tatcgtattc
    21541 ctggatgata attagccgtt gctgttaaac acttctcttt tagcggcaat ttttgcatta
    21601 ttcattttcc acttttgcta taaggatgtc cttgatgtct gaatttgatg cccagcgcgt
    21661 tgccgaacgt attgatattg tgttagatat ccttgtggca ggcgattacc actctgcgat
    21721 ccataatctc gagatactta aagcagaact cttgcgccag gttgctgaat ctacgccaga
    21781 catcccgaag gcaccgtggg agatttaaca atcgtgcctt caggttttcc tatttgagga
    21841 tgcccggcgc gtcagtattt ttttatttag tattataacg ttataagaat tacagcgatg
    21901 aattcccgac aacaaactat tctacagatg gtcattgacc agggtcaggt tagcgtaacc
    21961 gatctggcaa aagccactgg agtttctgaa gttaccattc gccaggatct caacaccctc
    22021 gaaaaactga gttacctccg ccgtgcacat ggctttgcag tttcgcttga tagtgatgac
    22081 gtcgaaaccc gtatgatgag caactatacg ctgaagcgtg aactcgccga gtttgccgcg
    22141 tcactggttc aaccgggcga aaccatcttt atcgaaaatg gcagcagcaa tgccctgctc
    22201 gcccggactc tgggggagca gaagaaaaat gtcactatca tcacggtcag cagctacatc
    22261 gcgcatttgc tgaaagacgc gccttgtgaa gtcattttgc tcggtggcgt gtaccagaaa
    22321 aaaagcgaaa gtatggttgg cccgttgaca cgccagtgca tccaacaggt gcatttcagc
    22381 aaagcattta ttggtattga tggctggcaa cctgaaactg gattcaccgg tcgcgatatg
    22441 atgcgtaccg atgtggtcaa tgccgtgctg gaaaaagagt gcgaagcgat agtcctgact
    22501 gacagctcga aatttggtgc tgtacattca tactccatcg gtcccgttga gcgattcaat
    22561 cgcgtgatta ccgattcgaa aatacgcgcc agcgatctga tgcatcttga gcacagtaaa
    22621 ctcaccgttc acgtcgttga catttaacca tttcccttct ctacggatga tttgcagttt
    22681 ggcaaatcat ccgctctaag atgattcctg gttgataatt aagactattt acctgttatt
    22741 aacacgctta agatataaaa ttattatcag cgatataaca ggaagtcatt atcacctgcg
    22801 tgatataacc ctgcgcgcga gcagatttca cggaataatt tcaccaggct tattcttagc
    22861 tattatagtt atagagagct tacttccgtg aatcataaat tcaggagaga gtattatgtt
    22921 tgtaacgagc aaaaaaatga ccgcggctgt tctggcaatt actttggcaa tgtctctgag
    22981 tgcctgttct aactggtcta aacgggaccg caacaccgca atcggcgcgg gtgcaggggc
    23041 attaggcggt gcagtactga ccgatggcag tacgttgggt acattaggtg gtgcagctgt
    23101 cggtggtgtt attggtcacc aggtcggtaa ataatcgtaa ataaatgtcg attatcgact
    23161 cgcaattata atacgcttga ttctgttcag tacgaaataa ctgaataata cgcacagtaa
    23221 aatattcgaa agccacggat atatccgtgg ctttttatgt taaccgcctg cgagttttac
    23281 cttcatccct ttcgcttcaa gtagtgattt taataaatca cgcttatcgc cctggatttc
    23341 aataactcca tctttaactg ctccgccgca gccgcatttt ttcttcagtt ccgctgcaag
    23401 ttttgtcagt tcggcatcat cgagatcgac accggtaatc aggcaaacgc ccttaccttt
    23461 acgtccactg gtctgacgct gaatacgcac cacaccgtcg cctttagggc gtacaggggc
    23521 cgctttgggc tcatcaatac gtccggtttc cgttgagtag accagacggc tgttggaatc
    23581 actcatcatg cactccgctg taaagaggcg ttgatcgctt tcagcgtctg cgctggatct
    23641 accgattgcg ttaccgggcg accaatcacc atataatcaa caccagccga caacgcctgt
    23701 tctggcgtca taatgcggcg ctggtcacca gcttcactcc cctgcggacg aatgcccggc
    23761 gtaaccagtt tgaactcctg accgaatacc tgtttaaagc gcacagcttc ctgagcagaa
    23821 cacaccacac catcaaggcc acatttttgc gtcagtgccg ccagacgttc tgcataatct
    23881 gcaggtgaca gtgtcatgcc aagatcgacc aggtcgctgg cttccatgct ggtcaacact
    23941 gtcacagcaa tcaaaagcgg tgcatctttg ccaaacggaa ccagtgcctc acgcgctgcg
    24001 gtcatcatac gcgccccacc agaggcatga acattcacca tccacacgcc taagtcagct
    24061 gcagcagcga cagcgtgcgc tgcagtgtta ggaatatcgt ggaatttcag gtcaagaaag
    24121 atatcaaaac cacgctgttg aagttcgcgc acaaactgtg gcccaaacaa tgtaaacatc
    24181 tctttgccga ccttcagacg acaatcgcgt gggtcgatct tgtcgacaaa ggacagcgcg
    24241 tcatcacgat tatgataatc aagggcaaca accacaggag aattcgtaac agcgcgggaa
    24301 gaagatgaag cagttaacgt catgaccaga ccttcttgat gatgggcacc ggaagtggcg
    24361 cgaaacaggt aaacggcgag cattctaccc gtggaaataa aaaattaaca gttgcgattt
    24421 ttcccctggc aggctttttc tgctccgtcg ttgccggagc acaatggaac atcaataact
    24481 agtatgttgt aactaaagtc ggattttttt aaaaattaca ggccatcaag accgcgaatc
    24541 ggtttaatgg ttgaccaggc ccgacaagac ggacaatgcc agtagagggt gtatgcggta
    24601 aaaccacatt tctggcagcg ataacgaggc ttactacgta ccttttcgcc aaccatgtca
    24661 cgcagcacca tcaggctctc tttggcacgc ccttcttccg cttcatttaa gtggtaatcc
    24721 attaacttat ggaacacacg catggtcgga tgacgctgaa gctggcgcgt aatatagacc
    24781 tgtgcggcct cactaccgtc gcgcgcttcg atgatatcag caagcatcaa ttcagcatcg
    24841 gcaccggtgt tctcttccac cgcgcgttgc aggaattctg cccattcggc agttttaccc
    24901 aactgctgat agcaggtttg caacatttcc agcgtttcgc tgaccagttc tctgtcctgg
    24961 gatatcacgc gttgcaaact ttcgacggct ttggcgtatt ctcctttcgc cataaacacg
    25021 cgtcccatca tgatggatac gcgggcgctg tttttatctg ccgccgcccc tttttttagc
    25081 aaggtcatgg cacgatcgag atcgtcgctg gccatatgct gcagggctaa ctcacagtag
    25141 aaatgggcaa tttcgacgcg ctgtttatct ttacccagct tcaccaggcg ttcggcaaca
    25201 tcaattgctt tctgccactc gctggtagcc tggtagattt gtagcaactg ttgcagcgcg
    25261 ccaatgcgga agtcagtttc atcggtcagc tgattgaaca tgtcttccgc gcggtcgtat
    25321 aacccggcgg ccatgtaatc acgccccagt tgttgaatcg ccaacagacg ctgttcatag
    25381 gtcagtgagg cgctttccat tagggtctga tggatgcgaa tagcgcgatc aacttcgcca
    25441 cgcgaacgga acaggtttcc gagcgtaagg tgggcttcaa cggtgcctgt atcctcttta
    25501 agcatatcga gaaacaggtc taccgcttta tcctgttgat tactaagcag gaagttaacc
    25561 cccgctacgt aatcacgcga caagcggttg gcttcatctt gcttgttttg ttgcgcactt
    25621 ctgcggccca tataccagcc ataggcagcg gctacaggca aaagcagaaa caacaactcc
    25681 agcatagaaa gttattcctt cgccgctgac gagtgcggca ctacagccac gtcagtcgcg
    25741 ggtgaaagct ggttttccag tcgctttatt ttacgttcag cgcgcgccag ggaaacacga
    25801 actcgcagcc agaacaggcc acaaatcaac caaccgatag caacccccgc agcaaacaat
    25861 accgccagca atgtggaaat acggtactcc ccttgcgcta acagataatt aaacgtcacc
    25921 tgttgatcgt tctgcgcacc caacgtgacc gaaatcacga agatcgctaa caccagtaag
    25981 aaaatgagta aatatttcac attacttccc gttatgtggt taaagcgaat aaattatggt
    26041 caacttaccg caatagccct ttaaagtacc attttcgcgc cgtctgcgaa atggcatcca
    26101 cgcgcattgt tgatgattta agggctaaat tcggaaaatc aaccagcgtt aactttcttg
    26161 ttctcgttgc gctatttcgc gattttcttc cgcaggtggt gttaatggcc cacaaattcg
    26221 ttgcgcaagc cacgttgcca ccgccaccag cgcccacgaa atcaacgtag ctactaccag
    26281 atcgcgtggc caatgcatcc cgagcagcag gcggcttccc atgactcccg ttgcccagac
    26341 cagcaagata gcaatggtta acgttcgccg acgcggccac agcaaaccaa cggccagcag
    26401 tgcccaactg gcagcaaaca tcgtgtgacc ggaaggaaag gcaaaccccg tctctttctg
    26461 ccagtgtgaa cgcaaatatt gtgggatatt tttctcttca gccaactgtt ctttcactag
    26521 atttccgcgt tctgctcgct ttaaagtgta gaactcatca accggaatat gatgtgtttt
    26581 ttccagccag ataacaaaag gtcgtggttc ctggactttg tctttgatcc aggatttaac
    26641 gccttgtccc acaatgatta cggccgccag aatggcaaat aatacaaagg cagccttaat
    26701 gcaaaaacgc agacaccaga gaaaccagcc gaataaaatc aaatgtgtaa tgacgcccca
    26761 gggctgggtg acagtttcag taacccaaaa agccgctttt agtagccaac tttgttctcc
    26821 aggttgccaa cgccagccag aaatccatac ggctactggc atgacaagca atagtgcagc
    26881 tcccactgcg gtacgtctgg caatcgaacg catggcctct cctttttgat aagtcccaca
    26941 atcataactg aaaacgccag ttccaggaaa aattgacaga tttgtgccat tccgtgaacg
    27001 atcgacgcgt cgtgattagg tgaacccctt ctcgttatgg caaaataagc caatacagaa
    27061 ccagcattat ctggagaatt tcatgcagct taaacgtgtg gcagaagcca aactgccaac
    27121 cccatggggc gatttcctga tggtgggatt tgaagaactg gcaaccggac acgatcatgt
    27181 cgcgctagtc tatggcgata tttccgggca taccccggta cttgcgcgcg tccattccga
    27241 atgtctgacc ggtgacgccc tgttcagctt gcgctgcgat tgtggcttcc agctcgaagc
    27301 ggcattgacg caaattgccg aggaaggccg tggtattttg ctgtatcacc gtcaggaagg
    27361 tcgtaacatt ggtctgctga ataaaatccg cgcttacgca ctgcaggatc aaggttacga
    27421 taccgtagag gctaaccatc agttaggctt cgccgctgat gagcgcgact tcactctttg
    27481 cgctgatatg ttcaaactcc ttggcgtcaa tgaagtccgc ttgttaacca ataacccgaa
    27541 aaaagtcgaa attctgaccg aagcagggat taatattgtt gaacgcgtac cattgattgt
    27601 aggtcgtaac cccaataacg aacattatct cgataccaaa gccgagaaaa tgggtcattt
    27661 gctgaacaaa taaccctctc gcattgtgga attaatttgc ttgccggaag caaaataacc
    27721 ggcaagcaaa tatttgttac ttcagcatat tacgaatgac ataatgcaaa atgccgtcgt
    27781 tctggtagta ggtcaactcc gtcgcggtgt cgatacgaca acggcagggt acgacctcct
    27841 ggctaccatc cgcgcgcgta agcgtcaccg gaaccgtcgc accgggttgt agattttgca
    27901 gatcgccaat atcaatcttc tcttccccgg ttagtcctaa cgttttacgc gttacgcctt
    27961 gcggaaattc cagcggcagg atgcccatgc caattaaatt cgaacggtga attcgttcaa
    28021 acgattcggc aatcaccaca cgaattccaa gcagacgagg accttttgcc gcccagtcac
    28081 ggcttgagcc tgaaccatac tctttcccgg caatcaccgc cagcggcgtt tgttcctgct
    28141 tatagcgcat cgcagcatca taaatagaga ctacgtcgct gtcaggtaaa tgccgcgtca
    28201 tccccccttc aacgccaggc accatttcat tacggatgcg aatattggcg aaggtgccgc
    28261 gcatcatcac ttcatggtta ccacgccgcg aaccgtagga gttaaagtct tttcgctcaa
    28321 caccccgacc ttgtagatat cgacccgctg ggctgtcggg cttaatactg cccgccggag
    28381 agatatggtc agtggtgact gaatccccca gcattgcgag gatccgcgca ccgtgaatat
    28441 cttccactgg tgctggtgtt gcctgcattt catcaaagaa aggcgataag cgaatatagg
    28501 ttgagtcctc ctgccaaccg taggtatcgg atcgtgtgac gttaatttcc ttccactctg
    28561 ctgtgccttc aaaaacttct gcgtactctt tgcggaacat ttctgtggag acttgttcta
    28621 ccgcacgggc aatttcttgt gccgatggcc agatatcttt cagataaacc ggatcgcctt
    28681 tacgatcatg accaataggc tcagaagcca ggttgatatt catatttccc gccagcgcat
    28741 aggcaaccac cagcggcggc gaggccagcc agttggtttt aaccagcgga tggatacggc
    28801 cttcaaagtt acggttgccg gacagcactg caccgacggt taaatcgcct tttttgattg
    28861 ccgtttcgat aggatcgggc agcggcccag agttaccaat acaggtagta caaccgtatc
    28921 ccacaaggtt aaaccccagt tcgtcgagaa acggtgtcag tttcgctttt gccagatagt
    28981 cagaaacaac tttcgagcct ggtgccagcg acgctttgac ccacggttgc cgcttgaggc
    29041 caagaattac ggcttttttc gccagcaagc ctgcagccat cagcacactt gggttagagg
    29101 tgttggtgca cgaggttatc gcagcaatga ccacagcgcc atcaggtaac tgatactgat
    29161 gtccgttcat aacataatcg accggctggc gatctttatg cgtggcattc acttccagtt
    29221 cgttactggc ggcaaatact tttggtacat cgggcagtgc aacgcgatcc tgtgggcgtt
    29281 ttggccctgc caggctcgct tcaacgtcat tcatatccag ttctaacgta ctggtaaaaa
    29341 tcggttcatc gcccgggtta cgccacatgc cctgcgcttt ggcatatttt tcgaccaact
    29401 caacctgatc ttcgctgcgc ccgcttaaac gcatgtaatc gagggttaca gcatcgattg
    29461 ggaagaagcc acaggtggca ccatattctg gcgacatatt ggcaatggtg gcgcgatccg
    29521 ccagcggtag tgaatccaga ccatcaccat aaaattcgac gaatttctcc accacgccat
    29581 gtttgcgcag catttgggta acagtgagaa ccaggtctgt ggcggtaata ccttcacgta
    29641 atttaccggt aagtttaaag cccactacgt ccgggataag catggaaacc ggctggccta
    29701 acattgcggc ttctgcttcg atcccaccca cgccccaccc cagcacgcca aggccgttga
    29761 tcatagtggt gtgcgagtca gtaccaacga gtgtatccgg ataagcaatc cattcaccgt
    29821 cctgcaattc actccacact gctttgccga gatattcgag gttaacctga tggcaaatgc
    29881 ctgtgcctgg cggcacgacg ctaaaccgac tgaacgcttg ctttccccat ttcaggaaca
    29941 cataacgttc gtggttgcgc tccatttcca ggcgtacgtt ttcttcaaat gcctcatcat
    30001 caccaaaacg atcgacggtc accgagtggt caatgaccag gtcgaccggt gagagcgggt
    30061 taacctttgc agtatcgccg ccgaggcgtt taaccgcttc gcgcattgcc gccagatcaa
    30121 caacggcagg tacgccggta aagtcctgca tcaacaccct tgccgggcgg taggcaattt
    30181 cacggtcagc atgggcattt ttcagccatc ctgccagcgc gtggatatcc tcttcggtaa
    30241 ccgagttacc atcctgccag cgcagcaggt tttcgagcaa aactttgagt gacttgggta
    30301 gacgggtgat atcgcccagt gatttagcag caagcggcag gctgtagtag tggtaagttt
    30361 tatctttggc ctgcaacgtg tccttactgg cttctcgtag ggttgacgac atagctcctc
    30421 cttaatgaca gggttgcgaa gccctgattc tcttcagggt gaatattaaa gataacacaa
    30481 acatcgcgta acgatttgat aacaacccaa attgataaaa gaggagaaac cttgtggcaa
    30541 agcaaatgac aaccccgccg cagcggggtc aaggatcagt gagtaatgat ccagaggagt
    30601 tcggcccaga agagcatcga cagagaaaac gcgcccatcc aggaccaata tttcatattt
    30661 gtgttgatca taggctacac ccttaacggt tattattttg tgttgagata actcaagctg
    30721 ttattgctgc ggtgtattaa aagcccttta attcattaaa aatgggtaat aagaatgatg
    30781 ataattattg tttcggctga tcatcttcag caaacagttc tataaaggct tgctgctgcg
    30841 atgttagttg ccagtcgtct ggaataacga cggaacattc ccgtttcgtt tctgtttcat
    30901 tgtcactatg ctggcataat ggtgacgact ccagttgctc ctgaccgacc atagttatcc
    30961 ccacactttc caaatcagca gtgcgacaac cacccagaaa agtgcagaac caagaaaaac
    31021 cgccagccag gctttacgtt ttaattcagg atcgcggcgg ggttcctgat ttccagaagg
    31081 cattgctaac ctcatataat cgccatcact tatcagcaag acgttgaacg attggtgtaa
    31141 ttaatcatac gatgagacaa atcctaaaga aactttagt
//
LOCUS       DAJTZL010000030        31084 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.030, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000030 DAJTZL010000000
VERSION     DAJTZL010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31084
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.030"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..278
                     /locus_tag="OO544_001223"
     CDS             <1..278
                     /locus_tag="OO544_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005153149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HCR6766863.1"
                     /translation="SVMCDGLLVGNAEIIPFSPRRYLYHAYLAYMRAHGFGKPVTLTR
                     FGKDMPGAMAEYGREYMKRKTKHGLRSNVTLTEDSEDWMPSCAIGHK"
     gene            582..827
                     /locus_tag="OO544_001224"
     CDS             582..827
                     /locus_tag="OO544_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6766864.1"
                     /translation="MTTKHAKKPQSHALDLTEHWLRVAIKIIDRNAGEGYAKAHPELI
                     SAFMTTTAANFATLTEREIAEAEQVTTINVKTGEQTA"
     gene            824..1246
                     /locus_tag="OO544_001225"
     CDS             824..1246
                     /locus_tag="OO544_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001758993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCR6766865.1"
                     /translation="MTAQIAAYGRLVADPQLKTTSKGTQMTMASMAVPLPCSQADDGT
                     ATIWLSVLAFGRQADALAKHQKGELVSVAGNMQVSQWTGQNGETRQGWQVIADSVISA
                     RTARPGGKKGQQGQATDALNRAKQQAGNDDPYGDNIPF"
     gene            1906..3773
                     /locus_tag="OO544_001226"
                     /pseudo
     CDS             1906..3773
                     /locus_tag="OO544_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000860399.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
     gene            4032..4313
                     /locus_tag="OO544_001227"
     CDS             4032..4313
                     /locus_tag="OO544_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6766866.1"
                     /translation="MTEAEILGLIRRVAGISQQVDEQAMQPDSVTAENYARVVDEVMR
                     RDGIELNGVDMRNIRTRVLELLAYRRRSQQRRESAKNTYQWRKPEHLRR"
     gene            4953..5273
                     /gene="clpS"
                     /locus_tag="OO544_001228"
     CDS             4953..5273
                     /gene="clpS"
                     /locus_tag="OO544_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HCR6766867.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            5304..7580
                     /gene="clpA"
                     /locus_tag="OO544_001229"
     CDS             5304..7580
                     /gene="clpA"
                     /locus_tag="OO544_001229"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HCR6766868.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(7986..8073)
                     /locus_tag="OO544_001230"
     tRNA            complement(7986..8073)
                     /locus_tag="OO544_001230"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8037..8039),aa:Ser,seq:gga)
     gene            complement(8327..8545)
                     /gene="infA"
                     /locus_tag="OO544_001231"
     CDS             complement(8327..8545)
                     /gene="infA"
                     /locus_tag="OO544_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HCR6766869.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(8830..9534)
                     /gene="aat"
                     /locus_tag="OO544_001232"
     CDS             complement(8830..9534)
                     /gene="aat"
                     /locus_tag="OO544_001232"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HCR6766870.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGAPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWHEDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFISHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQK"
     gene            complement(9576..11297)
                     /gene="cydC"
                     /locus_tag="OO544_001233"
     CDS             complement(9576..11297)
                     /gene="cydC"
                     /locus_tag="OO544_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HCR6766871.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGGAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTVGLSFLDF
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRITDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALSEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(11298..13064)
                     /gene="cydD"
                     /locus_tag="OO544_001234"
     CDS             complement(11298..13064)
                     /gene="cydD"
                     /locus_tag="OO544_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HCR6766872.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWAVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEVELASTDPVTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEHELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGQIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(13187..14152)
                     /gene="trxB"
                     /locus_tag="OO544_001235"
     CDS             complement(13187..14152)
                     /gene="trxB"
                     /locus_tag="OO544_001235"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HCR6766873.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            14697..15191
                     /gene="lrp"
                     /locus_tag="OO544_001236"
     CDS             14697..15191
                     /gene="lrp"
                     /locus_tag="OO544_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HCR6766874.1"
                     /translation="MVDSKKRLGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            15326..19393
                     /gene="ftsK"
                     /locus_tag="OO544_001237"
     CDS             15326..19393
                     /gene="ftsK"
                     /locus_tag="OO544_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HCR6766875.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTAAQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQPQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPVQQPYYAPAAEQPVQQPYYAPAPEQPVAGNAWQAE
                     EQQSTFAPQSTYQTEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPVVEETKPARPPLYY
                     FEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVETAAAVSPLAS
                     GVKKATLATGAAATVAAPVFSLANSGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYG
                     IKLPSQRAAEEKAREAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQH
                     DVPVNAEDADAAAEAELARQFAQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGP
                     HEPLFTPIVEPVQQPQQPVAPQQQYQQPQQPVAPQQQYQQPQQPVAPQPQYQQPQQPV
                     APQQQYQQPQQPVAPQPQYQQPQQPVAPQQQYQQPQQPVAPQPQDTLLHPLLMRNGDS
                     RPLHKPTTPLPSLDLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGP
                     VITRFELNLAPGVKAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTV
                     YLREVLDNAKFRDNPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAM
                     ILSMLYKAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMER
                     RYKLMSALGVRNLAGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLV
                     DEFADLMMTVGKKVEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAF
                     TVSSKIDSRTILDQAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKA
                     RGRPQYVDGITSDSESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIG
                     YNRAARIIEQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            19548..20159
                     /gene="lolA"
                     /locus_tag="OO544_001238"
     CDS             19548..20159
                     /gene="lolA"
                     /locus_tag="OO544_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HCR6766876.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            20170..21513
                     /gene="rarA"
                     /locus_tag="OO544_001239"
                     /gene_synonym="mgsA"
     CDS             20170..21513
                     /gene="rarA"
                     /locus_tag="OO544_001239"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HCR6766877.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLGTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVNDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            21604..22896
                     /gene="serS"
                     /locus_tag="OO544_001240"
     CDS             21604..22896
                     /gene="serS"
                     /locus_tag="OO544_001240"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HCR6766878.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            23135..25579
                     /gene="dmsA"
                     /locus_tag="OO544_001241"
     CDS             23135..25579
                     /gene="dmsA"
                     /locus_tag="OO544_001241"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HCR6766879.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSAIPTKSDEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPEWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLVNQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGINNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDAKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            25590..26207
                     /gene="dmsB"
                     /locus_tag="OO544_001242"
     CDS             25590..26207
                     /gene="dmsB"
                     /locus_tag="OO544_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HCR6766880.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            26209..27072
                     /gene="dmsC"
                     /locus_tag="OO544_001243"
     CDS             26209..27072
                     /gene="dmsC"
                     /locus_tag="OO544_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HCR6766881.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDSWAMRLLPAISVLALVVSGVVSVMQGAELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(27108..27734)
                     /locus_tag="OO544_001244"
     CDS             complement(27108..27734)
                     /locus_tag="OO544_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR6766882.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            28049..29197
                     /locus_tag="OO544_001245"
     CDS             28049..29197
                     /locus_tag="OO544_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6766883.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITAMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            29407..30837
                     /locus_tag="OO544_001246"
                     /pseudo
     CDS             29407..30837
                     /locus_tag="OO544_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            complement(30838..>31084)
                     /locus_tag="OO544_001247"
     CDS             complement(30838..>31084)
                     /locus_tag="OO544_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6766884.1"
                     /translation="DVDAELDAVLMGAGIGYLLYEQIKEYLDTGRLECVLEDWITERP
                     GFQIYYPNRQYMSCGLRAFLDYVKTGQICQSQRHRPQ"
BASE COUNT         7870 a         7502 c         8287 g         7425 t
ORIGIN      
        1 cgtcggtaat gtgtgatggt ctgttagtgg gtaatgccga aattattccg ttcagtccgc
       61 gcaggtatct ttatcacgcc tatctggcat atatgagggc acacggattc ggtaaacctg
      121 taacactgac gcgcttcggt aaagatatgc cgggggcaat ggcggaatat ggcagggagt
      181 atatgaaacg gaaaacgaag cacggtttgc gttcaaatgt gaccctgaca gaggattcag
      241 aagactggat gccatcatgt gcaataggtc acaaatgacg atggaaaaaa ttaaacttat
      301 ggaataactg ttcaccactg ttcaccctgc aataaatgtc ttttatatca gtatattata
      361 gggtgaacag ttatttatga actgttcacc aaactattca ctgttcacct ttttgattct
      421 ttattgagct ttaagggtga acagtggtga acagttggtg aatagttttt gtgaaactgt
      481 taacccatta acatcatgaa taaaaaggga aaattgcaaa aggtgaacag gtgaagggtt
      541 aaaatgcaaa aattttattt tattgctgtg aggtaaagcc tgtgacaacg aagcacgcca
      601 aaaaaccaca atctcacgcc cttgatttga cagaacactg gctgagggtg gcgataaaaa
      661 tcatcgaccg caacgccggg gaaggatacg cgaaagcaca tcccgaactg ataagcgcat
      721 tcatgaccac gacggcggca aactttgcca cgctgacaga acgggagatt gccgaagcgg
      781 aacaggtgac aaccatcaac gttaaaaccg gagagcagac agcatgacag cacagatagc
      841 ggcttacggg cggctggtgg ctgacccgca gttaaagacc accagcaagg gtacgcaaat
      901 gacgatggcg agtatggcgg ttcccctgcc gtgcagccag gcagatgacg gaacggcgac
      961 gatatggtta tccgtcctgg cgtttggcag acaggctgac gcactggcaa agcaccagaa
     1021 aggcgagctg gtgagcgtgg cgggtaacat gcaggtaagc cagtggacag gccagaacgg
     1081 agaaacgcgg cagggctggc aggttatcgc agacagtgta atcagtgcca gaacggcgcg
     1141 accgggcggt aaaaaaggcc aacaggggca ggccactgat gcactgaaca gggccaaaca
     1201 acaggcgggg aatgatgatc cgtacgggga taacataccg ttttaagcaa cgagtgacag
     1261 aagccggagc aatccggctt ttttgtaggt actcctggtg ggggtggcct gtccacgggg
     1321 cgggcggttc gcggaaaaag gcgtattttt tgatttttat gtcaccatca ccaccagttt
     1381 aagttattga tatatggcaa aataaaaatt tttagtgtcg aattaggttg ttttttgttc
     1441 gacgccggaa cgttcccgaa agcatttaca aaaaacaggc gcaaaaaaaa gcgccccgat
     1501 ggtgccaccg gaggcgcttt gtcagcgaca aaggatactg ccactaccga aatgatgagt
     1561 cttaattgtg ctggcgtggc aataattagt caattctttt gctggtatga caaaaatcag
     1621 aaaacacata atctgatttt tagatagaaa atgatttatt taccagtttt atcgatcaat
     1681 aatgatgccc gttaatcata acggaggcag acttatgcca gagaacaaca gcagaaaacc
     1741 ggataaaagt gcaacagtgc acatagacgc cggaactatg gagaagctcg aacgctatca
     1801 gcagttcatt aaaagtcatc accctggaat gccagtaccc acgaaagggc agattgcgag
     1861 aagcgcggtt gaatactggt acagggcaac gttaggagcc tggctatgaa aacatggttt
     1921 tccattaagg ctatggctga tgttgtacat gtacgcattt atgacgagat cggcgggtac
     1981 ggtgtaaaag catcggcact tactgacgag atcaacgcgt gcggtaatgt gcctgaaatc
     2041 catcttcgca tccattcacc tggtggcgac atctttgaag ggctggctat ctataacgcc
     2101 ctgaaaaatc atccggcaaa gaaaattgta cacattgaag gcatggcggc ttctatggcc
     2161 tcgtttattg ccatgtgtgg cgatcacatc gttatgcctg aaaacgcgat gatgatgata
     2221 catgcccccc gtggtgttac tgccggagtg tcgggcgacg ttcgccgctt tgctgacctg
     2281 atggacaagc tgggcgacac gatggcggaa acctacgccg gaagaacggg caggagcaaa
     2341 caggaaatca ccgccatgat ggaggcggaa acctggatgg atggcaatga gtgtaaggct
     2401 aatggcttcg cagatgaggt tatacccgcg attacagcaa tggccagaat tgaatcaaaa
     2461 cgaatcggag atttttcgaa tatgccggaa aatattaaaa gcatgatcag ccagaaaact
     2521 ggcagcggag aacaggaacg actcaacggg atacgtgaat tgtttggcac gttcaatgga
     2581 agatataacg acctggctat aagttgtctt gctgattcag gctgtagcgt tgaggatgca
     2641 cgcgaacgcc ttttactcac tatgggtaaa gaatcgacac cgacaaacaa aaccaccccc
     2701 gcaaatcttt actacgcgta cacggataac ggcaacataa ccggcgatgc aatgcgtcag
     2761 gggcttaatg cgcgtctggg acatgaacag gcagaacgcg gcaaccctta cgccatgatg
     2821 agccttttcg atatggcaca ggcatcatta acccatcgcg gcataagcac aggcagctac
     2881 agcacacgct cgcagatagt aaacgcggca ttcacccaca gcagcagcga ttttacttat
     2941 atccttgctg gtggtgctga aaaatccgtg aacacagcgg cgaaacgttc cgccagtgga
     3001 cgaaaaaagg ttcattgtca aacttccatg aggcacgtcg tgttggcctg aatggcttct
     3061 ctacattaaa caaagtgccg gaaggcgcgg aatataaata catcaccacc agtgaccgtg
     3121 gcgaaccaat cgcgctggca acttacggaa atattttcag cattacccga caggcgatta
     3181 ttaacgatga cctgaatcag ttaacagtta tcccacaggc aatggggcga gcagccgcca
     3241 gaacggtggg taatctggtt catctggtgc tgacaggaaa cacaaagctt tctgacggta
     3301 taccgctgtt cagcaaaaaa cacggaaatg atattgataa cggagtcagc actacagggc
     3361 ttggcattgc acgccgtgca atgcgcacac agagggatga aaacggtgag gtgctgaata
     3421 ttgtgccaaa attccttctt gttcctgcgg ctctggaaga cagagcgttg caaatggtta
     3481 actcaaccac gcctttcggt gccgatacaa acagtggttc atataacccg taccacaaac
     3541 aactggatat tattgtcgat ccccgccttg atgaagtcag tgataagcaa tggtacatgc
     3601 tttcagcaca gggaacggac acaattgagg tggcttatct tgacggtaat gacgagcctt
     3661 accttgagca gcaggagggc tttattgttg acggtgtggc ctggaaggtc cgcattgatg
     3721 ctggcgttgc tgctctggat tatcgcggaa tggtcagagc gatttcttca tgagaacaag
     3781 gcggcagcag ccgccttttt tgcgggtcct cctggtgggg ggggcctgtc cacggggcgg
     3841 gtcggcgcgg aaaaaagcgc atttttgtgg ttttatcgtc atcatcatca tgtgtgtaac
     3901 ctgttgtttt taatgtagtt gatgcaaaaa agatgatgat tgtggttaat ttttgttcga
     3961 catcttttag cgtgacagat tctttacaaa aaatctgagc ttgttttctt aaccagcgcg
     4021 atgggggcac aatgacagaa gccgaaatac tgggattaat ccgccgcgtc gccggaatca
     4081 gccagcaggt tgacgaacag gccatgcaac cggacagcgt gaccgctgaa aattatgcgc
     4141 gtgtggtgga tgaggtaatg cgccgtgacg gtattgagct taacggcgtg gatatgcgca
     4201 acatacgaac cagagttctt gagttgctgg cataccgtcg ccgttctcaa caacggaggg
     4261 agagcgcgaa aaatacttac cagtggagga agccggagca cttacggcgg taactggttg
     4321 atattaccga aagtgcaaaa atgcacttag caactggtgg cgagttgcag atctgcaact
     4381 cgactatgaa actacggaaa ctacccgtag tttgggtagt aagagtaaca cccagatttt
     4441 ggggcttact cgcgataccc aaataaaggg tatcggtgga agaaatatcg tttctcatat
     4501 gtgagtaccg agggcggaat tccgccttcg gttacttatt gtgcgcatgc acagggaggg
     4561 gcgggtcaaa tctctgtacc ctgacgtctt ccggactgcc agccccatcg attttttata
     4621 cccgcgaaaa atgaaaaacg cttcacgctg gtgggcctga tgccgataac tggtttcatg
     4681 actgatagcc ggaaaatgat aacgattaaa gcgtgttgat ctttgttcgt ggttgttcga
     4741 tattgttcgt ggaacggtgt agttaatggt gtagttaatt ttactgtttt tgacgagctt
     4801 attgtttgca actcaatgaa aaataaaggc taataagcca aatgctataa atgatagtta
     4861 tctatcatgt ggagtagatt ggtcaggcaa ataagctctt gtcagcggca gggcgttctg
     4921 ccgataaccg taaccgaaga tgataactga caatgggtaa aacgaacgac tggctggact
     4981 ttgatcaact ggcggaagaa aaagttcgcg acgcgctaaa accgccatct atgtataaag
     5041 tgatattagt caatgatgat tacactccga tggagtttgt tattgacgtg ttacaaaaat
     5101 tcttttctta tgatgtagaa cgtgcaacgc aattgatgct cgctgttcac taccagggga
     5161 aggccatttg cggagtcttt accgccgagg ttgcagaaac caaagtggcg atggtgaaca
     5221 agtacgcgag ggagaatgag catccattgc tgtgtacgct agaaaaagcc tgaatgcagg
     5281 cataaaaatt gggggaggtg cctatgctca atcaagaact ggaactcagt ttaaatatgg
     5341 ctttcgccag agcgcgcgag caccgtcatg agtttatgac cgtcgagcac ttgttactgg
     5401 cgctgctcag taacccatct gcccgggagg cgctggaagc gtgttctgtg gatttggttg
     5461 cgctccgtca ggaactggaa gcctttattg aacaaaccac acccgttctg cctgccagtg
     5521 aagaggagcg cgacacacag ccgacgctga gttttcagcg tgtactgcaa cgtgcggtct
     5581 tccatgtcca gtcctccggt cgcaatgagg ttaccggtgc aaacgttctg gtcgctatct
     5641 ttagcgaaca ggagtcgcag gcggcatatc tgttgcgtaa acatgaagtc agccgtctcg
     5701 atgtggtgaa ttttatctct catggcacgc gtaaagacga gccgacacag tcttctgatc
     5761 ctggcagcca gccaaacagc gaagaacaag ctggtgggga ggaacgtatg gagaatttca
     5821 cgacgaacct gaatcagctt gcgcgcgtgg gcggaatcga cccactgatt ggtcgtgaga
     5881 aggagctgga gcgtgctatt caggttctct gccgtcgccg taaaaacaac ccgctgctgg
     5941 tgggggaatc tggtgtcggt aaaaccgcga ttgcggaagg tcttgcctgg cgaattgttc
     6001 agggcgatgt gccggaagtg atggctgact gtacgattta ctctctcgat atcggttctc
     6061 tgttagcggg cacaaaatat cgcggcgact ttgaaaaacg ttttaaagcg ttgctcaagc
     6121 agctggagca ggacactaac agcatcctgt ttattgatga gatccacacc attatcggtg
     6181 cgggggcagc gtctggtggt caggttgatg cggctaacct aatcaaaccg ttgctctcca
     6241 gcggtaaaat tcgtgtaatt ggttcgacaa cctatcagga gttcagcaac attttcgaga
     6301 aagaccgtgc tctggcgcgt cgcttccaga aaattgatat tactgaaccg tcaatcgaag
     6361 aaactgttca aatcatcaat ggcctgaaac cgaagtatga agcgcaccac gacgtgcgtt
     6421 ataccgcaaa agcggtgcgt gcggcggtag agctggcggt gaaatacatc aatgatcgtc
     6481 atctgccgga taaagcgatt gatgttatcg acgaagcggg cgctcgcgca cgcctgatgc
     6541 cggtaagcaa acgcaagaaa accgttaatg tggcggatat tgagtccgtg gtggcccgta
     6601 ttgcacgcat tccagagaag agtgtttctc agagtgaccg cgataccctg aaaaacctcg
     6661 gcgatcgcct gaaaatgctg gtcttcggtc aggataaagc cattgaggcg ctgactgaag
     6721 ccattaagat ggcgcgtgca ggtttaggtc acgaacataa accggtcggt tcgttcctgt
     6781 ttgccggtcc taccggggtc gggaaaacag aggtgacggt acagctttcg aaagcgttgg
     6841 gcattgagct tctgcgcttt gatatgtccg agtatatgga acgccatacc gtcagccgtc
     6901 tgattggtgc gcctccggga tacgttggtt ttgatcaggg cggtttgctg accgatgcgg
     6961 taatcaagca tccacatgcg gtgctgctgc tggacgaaat cgagaaagcg catccggacg
     7021 tgttcaatat tctgttgcag gtgatggaca acggtacgct gaccgataac aacggacgca
     7081 aagcggactt ccgtaacgtg gtgctggtga tgaccaccaa cgccggggta cgggaaactg
     7141 agcgcaaatc cattggtctt atccaccagg ataacagcac cgatgcgatg gaagagatca
     7201 agaagatctt tacaccggaa ttccgtaacc gtctcgacaa cattatctgg tttgatcatc
     7261 tgtcaaccga cgtgatccat caggtggtgg ataaattcat cgtcgagttg caggttcagc
     7321 tggatcagaa aggtgtttct ctggaagtga gccaggaagc gcgtaactgg ctggccgaga
     7381 aaggttacga ccgggcaatg ggcgctcgtc cgatggcgcg tgtcatccag gacaacctga
     7441 aaaaaccgct cgccaacgaa ctgctgtttg gttcgctggt ggacggcggt caggtcaccg
     7501 tcgcgctgga taaagagaaa aatgagctga cttacggatt ccagagtgca caaaagcaca
     7561 aggcggaagc agcgcattaa tctaattgtt aggtaggttg gtgaagtccg taatctcgaa
     7621 agaggttacg gactttttgt ttatggggtg gaacggtgaa aaccctattt ttggaggtga
     7681 aggtaatttg ttgataatta gtgctgctgg aaaaggtaag gataaaaaag ggtgacagca
     7741 ggaaaatgag atggttttgc tttattaaca acggactaaa cgtgtagtat ttgagttcac
     7801 tgccgtacag gcagcttaga aatcgacggg gtgcggtaaa acctttgcga acgcgttcac
     7861 tgccgtacag gcagcttaga aattcacagg taacatactc cacccgccca ccatgttcac
     7921 tgccgtacag aaagataaaa tgcgaaaaaa aagctcgcac tttcgtacga gctcttcttt
     7981 aaatatggcg gtgagggggg gattcgaacc cccgatacgt tgccgtatac acactttcca
     8041 ggcgtgctcc ttcagccact cggacacctc accaaattgt tttgctacca aacctcatgg
     8101 gtggcaacgg ggcgctacta tagggagttg gagtaaaacg gtcaagaaga attttaatga
     8161 taattattgt ttgctcatac tgtaaacaag ttgtgcagta tatctacatc gagacaagtt
     8221 acggacttat acttccaaag tacttcatac atatcacaaa ataaaaaggc cggttaaacc
     8281 gaccttttac tcgttctttc tcttcgccca tcaggcggta aaacaatcag cgactacgga
     8341 agacaatgcg gcctttgctc aggtcgtacg gggtcagttc aacagtcact ttgtcgcccg
     8401 tcaggatgcg gatgtagttt ttgcgcattt taccggagat gtgtgcagta accacgtgac
     8461 cgttttctaa ctctacgcgg aacatggtat taggcaacgt ttcaagaacg gtaccttgca
     8521 tttcaatatt gtcttctttg gccatctaat cctctggggt atcactaccg taatttgaac
     8581 cggcaagata atgccgaagt tctgtaaata agtaaagatt tgcgcgctaa atcgcaacaa
     8641 acaggttcgg cacattactc cgaaaacaca cggctaagcc gcaccaaaag cgcaacgtat
     8701 aagggagcgg tgagataaac gatgggcgtt acctgacgcg aaaaattcct tatcggcagc
     8761 ggggtaatga gcataaccaa ctctgcgacc gcaattataa cactctgggg agaaatgtgc
     8821 cgaaaacatt catttttgtg gtgaaaacaa gcatcgtggc acccagaaat tattcggcaa
     8881 tcgtccgagg cgcatttgat tgagataatt cagataatcc cgacggggaa tttcgcaggc
     8941 accaagcgat gctgtgtgat cgttaaggac ctggcagtcg ataagcttac cgccatgact
     9001 gataaattcc tcacagaata ccagaagcgc cgttttagac gcattttcca tccggctgaa
     9061 catggactcg ccacaaaata gcgttccctg ggccacgccg tacataccgc cgacaagctc
     9121 atcttcatgc cagacttcaa tggagtgggc atgaccgagt tcgtgaaggc gatggtaggc
     9181 ttcgaccacg ccacgcgtga tccaggttcc ttcttcgcga tcgctggcac aaccttcaat
     9241 gacctgacca aaagcgtaat tcatcgtgac acgatagggc gagcgtttat gaaatcgctt
     9301 catactacgg ctgatatgca gtgattcagg ccaaagcacc gcgcgaggat cgggcgacca
     9361 ccagaggatg ggggcgcctg gagaaaacca cggaaaaata ccacgctggt aagccattaa
     9421 caggcgcgca gggctaagat cgccccccag agccagcagg ccgttaggct cacgtaatgc
     9481 gccttccggg gaagggaagg ctattgaatg gcgagaaagc tgaaccaggc gcatgaccgc
     9541 aaaactccac gcaagtcgga tcgttcaata atagcttaca aaccctgctt aaactggtaa
     9601 taacgcccct gtctggcaag cagttctgcg tgagtacctt gctcaataat ttgcccgttg
     9661 tccatcacta ttatttgttg gaaacgagag agtccgcgaa gtcgatgggt gaccattaac
     9721 accgttttct cacgcatcat ttctgcaagc aattcaagga tctggctttc ggttgtggca
     9781 tctaagcctt cggtaggttc atccagcaac accagtggcg catcatgtaa cagcgcacgg
     9841 gcgatagcca gacggcgcag ttcaccaccg gagagctggc gtccgccttc acctaaccaa
     9901 ctgttgagac ctgcgtcctc gagcagcttc tccagaccaa cgcgacgcaa gatctccgac
     9961 agagcctcat cactactgcc aggcgaggcg agtaacagat tatcacgcag cgtggcgcta
    10021 aacagatgca ctcgctgagg aacaacgctg atggtctgtc gtagagccgc ttcattcagg
    10081 ctggctatgg ggctatcgtt aagcaaaatc tcgccctgtt gcgggtccca agcgcgggtc
    10141 agcagttgta acagtgttga tttgccgcat ccggttcgcc cgagaatcgc aatatgttcc
    10201 ccggcgttta cctgaagaga aatcccttta agtgcctgtt gggattgctc cggataagtg
    10261 aactgaacat cccgtaatgt cagcgaaacg cgatcggcaa cacgagtttg ggtatcaggg
    10321 aaggtgactt ccggtttttg atccgttaaa tcagtgatcc gaacggcaga ggcaatgact
    10381 tgccccagat gctgaaatgc acccgttact ggtgccagtg cttcaaacgc ggctaacgcg
    10441 cagaagacaa acagggcaat taacgcgccg ggttgagcat tgccgccaac gccgccagac
    10501 gccatccaca gcatcaggat caccgctaac gcgccaatga gcagcattat cgcttgcgac
    10561 aatgcggtca gttcagattg acggcgttgc gcttccagcc attgaatttc tgtattctct
    10621 agttgcgtgc gataacgatc gctggcacca aaaatggtca gctcagcttg cccttgcagc
    10681 caggccgtca gttgttggcg atactgtccg cgaagatgag tcagattttg cccggtgctt
    10741 tttcccgcac gataaaacag cggtggcatc aggaaaagcg tcagtaacat aatgccgccc
    10801 agcgtaaagg cgagggtgaa atcaaggaaa cttaacccga ctgtcaccac cataatcacc
    10861 acaaaagcgc ccaccagcgg cgagataacg cgcaggtaaa gatgatcgag cgtatcaaca
    10921 tccgccacca cgcgattgag caattcgccc tgacgatagc gcgccagtcc ggcaggggag
    10981 aggggcaaca atttgctgaa ggtgtaaatg cgcagatgct gcaacacgcg gaaagtcgcg
    11041 tcgtgactta ccagacgttc aaaataacgc ccggcagtac gggtgattgc ggcaccacgc
    11101 acgcccgcag cgggtagcat atagttgaag ctgtacagtc cggcaccccc cgcaaccgct
    11161 gaggccgaga ggaaccagcc ggaaagtgtc aacagaccga tactggcgag cagcgtcaca
    11221 attgccagca caataccaag acttaacatc catttatgac gtttatacag tgccagatag
    11281 ggtagcaaag cgcgcattta aatctcctcc tgacgatggg ccagtaatgt ggcgaatggg
    11341 ccaccagcca cacttaattc cgcgtaacgt ccttgctcaa taatctgacc atcctgcatt
    11401 acccaaatga catcccagtc agcaagatct tctaactggt gggtgaccat taacgttgtc
    11461 tggcgcagag aggcggcatt cagcgcctcc attacgcgct gttcactgtg agcatcaagg
    11521 ctggcagcgg gttcatccaa cagtaatagc gaacagggat ttagtaacgc acgggccacc
    11581 gccacgcgct gcgcctgccc cacggaaagg cgggcagcct ggtcgccaac aggcgtatca
    11641 acgccttgtg gcaggagcgg tagaaactcg ctgacccagg cgttatccag cgctgcttgt
    11701 aattcatgtt cgctggcatc aggtcgcgcc agtagtacgt tatcccgtaa tgttgctgcc
    11761 ggtaattgtg gattttgccc aacccaggag agatgtttac gccatgattc tggtgataaa
    11821 tcgcgtaatt ctatcccgtt gattcgtagc gatccctgat atgagagaaa accagaaagc
    11881 gcgttcaaca gtgaactttt acctgaaccg ctgcgaccaa ccaacacggc acgttggcct
    11941 gctggcaaag taaagttcag cggtccggcc agcgttttac cttccggcga cgtgataaac
    12001 agatcctcgg cctcaatggt caccggatcg gtcgatgcta attccacctc gccacgctgc
    12061 ggatgggcga gcggggtttc cataaacgtt ttcagactgt cagctgcacc aacagcctgg
    12121 gctttagcat gataaaacgt accgagatcg cgtaatggct ggaaaaactc tggcgcaagg
    12181 atcagggcca gaaaacccgc agccagcgtc acgccagtat cgtagtgacc aaaatccagc
    12241 tcgccgagat aggaaaaacc aaagtagacc gccaccagag caattgacag cgaggtaaaa
    12301 aattcgagaa tgccggagga taaaaatgcc agccgtagca cttccattgt ccgttggcgg
    12361 aaatcttccg aagcagaacg aatactttca atttcagctt caccacgacc aaaaatacgc
    12421 aatgtttcca tgccgcgcag gcgatcgagg aaatgcccac ttaagcgagc aagagcgaga
    12481 aagttacgtc ggttagcatc ggcagccccc attccaacca gcgccataaa caacggaatt
    12541 aacggtgcag tgcccagcag aatgagcgcc gcagcccagt ttgaggggaa gattgccacc
    12601 acaatcagca acggcaccga cactgccagc gccatttgcg gcaggtagcg tgcatagtaa
    12661 tcatgcatat cgtcaatttg ctcgagtacc agcgtcgccc agctccccgc aggtttaccc
    12721 tgaatccacg ctggccctgc ttgttgcaga cggtcgagaa cctgacggcg gatggcaaag
    12781 cggatatgct gcccggcgtg ataacccacc cgttcgcgta accagaccgc ccatgcgcgc
    12841 agtacaaagg tcagaaccag taacgtaaag ggaagcagca gggcttcacg gggaatattc
    12901 tccataatca tatgttgcag aattcgcgcc atgaaccagg cctgggcaat gatcaatatg
    12961 ccgcttacaa agcccagcag acgagaaata ttcagccaac gttgggagat gacgctttgc
    13021 tgttttaacc agcgggttaa ctctttttga cgagatttat tcattgcacg cttagcaggt
    13081 gagttatcag aattatttgc agagcaatgt tacaacgggg aaaaaataaa ggcgacccat
    13141 agtcgcaagg tgtcgccttc tttacttttg ttactgattt gtaaaattat tttgcgtcag
    13201 ctaaaccatc gaggtagcgt tccgcatcaa gtgctgccat gcagccagta ccggccgaag
    13261 taatggcctg gcgataaatg tgatccatca cgtcgcctgc ggcaaagacg ccaggaatgc
    13321 tggtctgggt ggcattacca tgaatacccg actgtacttt gatgtagccg ttttccagtt
    13381 ccagctgccc ttcgaaaatc gcagtattcg ggctgtgacc gatagcaaca aacagaccgg
    13441 caacgtcgag tgactcgatg ttatcgctgt tttgcgtatc gcgcagacga acgccagtga
    13501 cacccatttg atcgccggtc acttcttcca gcgtacggtt ggtgtgcaga atgatgttgc
    13561 cgttctccac tttatccatc agacgcttaa taaggatttt ttccgcgcgg aaaccgtcac
    13621 ggcggtgaat cagatgcact tccgaagcga tgttagacag atacagcgcc tcttcaaccg
    13681 cggtattgcc gccgccgatg accgcaactt tctggttgcg atagaagaaa ccgtcgcagg
    13741 ttgcacaagc agaaacccca cggcctttaa aggcttcttc agagggcagg ccgagatagc
    13801 gtgcagaagc tccggtggca ataatcagcg cgtcgcaagt gtattcgccg ttatcgccat
    13861 tcagacggaa cggacggttt tgcagatcca ccttgttgat atgatcaaaa atgatctcag
    13921 tttcaaactt ggtggcatgt tcgtgcatgc gctccattaa taacggaccg gtcagatcgt
    13981 ttggatcacc aggccagttt tccacttccg tggtggtggt cagttggccg cctttttcca
    14041 tgccggtaat cagcacaggt tgcaggttgg cgcgcgccgc gtagacagca gcggtgtatc
    14101 ccgccgggcc tgaacccagg ataagcagtt tactgtgttt ggtcgtgccc atgagatccc
    14161 catagttgtt ggcagacaat gggcaggatt gtagggaatt tacagacgta aaaaaagagt
    14221 atgacgattt tgttaacaat ttgtgcaatc ggcagcatcg ataagcaggt caaattctcc
    14281 cgtcattatc acctctgcta cttaaatttc ccgctttata agccgattaa atgatgaata
    14341 aacgcccctg ttaatgaata tctggcatgt tgtactaaaa atcgatgttt tgctttgaca
    14401 atcccctggt gttttgcgaa aacattcgag gaagaaaaaa aacagtattc ttatatgcgc
    14461 ataaccatgc atgtaaatac catgtttacc gtgctagtga aatctacgta tggcgtggac
    14521 agacgccatt cgtgatgtcg atagctgcca caaggcaacg gtcttctcac cgtagaccca
    14581 ggcattgcgc gccgtgaatc ttcatgattt cggtctatcg tgacgggtag cgactctgaa
    14641 cagtgatgtt tcaggggcag acaggagtag ggaaggaata cagagagaca ataataatgg
    14701 tagatagcaa gaagcgcctt ggcaaagatc tcgaccgtat cgatcgtaac attcttaatg
    14761 agttgcaaaa ggatgggcgt atttctaacg tcgagctttc taaacgtgtg ggactttccc
    14821 caacgccgtg ccttgagcgt gtgcgtcggc tggaaagaca agggtttatt cagggctata
    14881 cggcgctgct taacccccat tatctggatg catcacttct ggtattcgtt gagattactc
    14941 tgaatcgtgg cgcaccggat gtgtttgaac aattcaatac cgctgtacaa aaacttgaag
    15001 aaattcagga gtgtcattta gtatctggtg atttcgacta cctgttgaaa acacgcgtgc
    15061 cggatatgtc agcttaccgt aagttgctgg gggaaaccct gctgcgtctg cctggcgtca
    15121 atgacacacg gacatacgtt gttatggaag aagtcaagca gagtaatcgt ctggttatta
    15181 agacgcgcta acacggaaca ggtgcaaaat cggcgtattt tgattacact cctgttaatc
    15241 catacagcaa cagtactggg gtaacctggt actgttgtcc gttttagcat cgggcaggaa
    15301 aagcctgtaa cctggagagc ctttcttgag ccaggaatac actgaagaca aagaagtcac
    15361 attgacaaag ttaagtagcg gccgccgcct tctggaggcg ttgctgatcc ttattgtcct
    15421 gtttgccgtc tggttgatgg ctgccttact aagctttaac ccttcggacc ccagctggtc
    15481 gcaaacggcc tggcatgaac ctatccataa tttaggtggg atgcccggtg cgtggttggc
    15541 agatacgctg ttctttattt ttggcgtgat ggcttacacc attcccgtca ttattgtcgg
    15601 cggttgttgg tttgcctggc gtcatcagtc cagcgacgaa tacattgatt attttgccgt
    15661 ttcgctacgc atcattggcg ttttggcgct catccttacc tcctgtggtc tggcggcaat
    15721 caacgctgac gatatctggt attttgcctc cggtggcgtc attggcagct tactaagcac
    15781 tacgctacaa ccactgctac acagtagcgg gggaactatt gcgctgctct gcgtttgggc
    15841 agcgggcctg acgttgttca ccggttggtc atgggtgacc attgctgaaa aactcggcgg
    15901 ctggatttta aacattctca ccttcgccag taatcgtacc cgtcgcgatg atacctgggt
    15961 cgatgaagat gagtatgaag acgacgaaga gtatgaagat gaaaatcacg gcaaacagca
    16021 tgaatcacgc cgtgcccgta ttcttcgcgg cgcgctagcg cgtcgtaaac ggttggcgga
    16081 aaaattcatt aatccgatgg ggcggcaaac agacgctgcg ttgttctccg gtaagcggat
    16141 ggatgatgac gaagagatta cctacactgc acgcggtgtg gctgccgacc ctgacgacgt
    16201 cctattttcg ggcaatcgtg caacgcagcc agaatatgac gaatacgatc cattattaaa
    16261 cggtgcgcca attaccgaac ctgtcgctgt agcagctgct gctaccacgg cggcacaaag
    16321 ctgggctgcg ccggttgaac ctgtgactca gacgccgcct gttgcctctg ttgatgttcc
    16381 accttcgcaa cctacagtag cctggcagcc tgtaccgggt ccacaaacgg gagagccggt
    16441 tattgctcct gcaccggaag gttacccaca gcagccacaa tatgcgcagc ctgcagtgca
    16501 atataatgag ccgctgcaac aaccagtaca gccacagcag ccgtattatg cacctgcagc
    16561 tgaacaacct gtgcaacaac cttactacgc tcctgcggcg gaacaacctg ttcagcaacc
    16621 gtattacgcc cctgcgccag aacaaccggt ggcaggtaac gcctggcaag ccgaagagca
    16681 gcaatccact tttgctccac agtctacata ccagactgag caaacttatc agcaaccagc
    16741 cgctcaggag ccgttgtacc aacagccgca acccgttgaa cagcagcctg ttgtggagcc
    16801 tgaacccgtt gtagaagaga caaaacccgc gcgtccgcca ctttactatt ttgaagaagt
    16861 ggaagagaag cgagcccgtg aacgtgaaca acttgcggcc tggtatcaac cgattccaga
    16921 accggttaaa gaaccagaac cgatcaaatc ttcgctgaaa gcaccttctg ttgcagcagt
    16981 acctccagta gaaaccgctg ccgctgtttc tccgctggca tctggcgtga aaaaagcgac
    17041 actggcgacg ggggctgccg caaccgttgc cgcgccagtc ttcagtctgg caaatagcgg
    17101 tggaccgcgt ccgcaggtca aagaggggat tggcccgcag ttgccacgac cgaaacgtat
    17161 ccgcgtgcca actcgccgtg aattggcgtc ttacggtatt aagctgccct cacagcgtgc
    17221 ggcggaagaa aaagcccgtg aagcccagcg caatcagtac gattctggcg atcagtacaa
    17281 cgatgatgaa atcgatgcga tgcagcagga tgaactggca cgtcagttcg cccagacaca
    17341 gcagcaacgc tatggcgaac agtatcaaca tgatgtgccc gtaaacgcag aagatgcaga
    17401 tgctgcggca gaggctgaac tggctcgtca gtttgcgcaa actcaacaac aacgttattc
    17461 cggcgaacaa ccggctgggg cgaatccgtt ctcgctggat gattttgaat tttcgccaat
    17521 gaaagcgttg ctggatgatg gtccacacga accgttgttt acgccaattg ttgaacctgt
    17581 acagcagccg caacaaccgg ttgcaccgca gcagcagtat cagcagccac aacagccggt
    17641 tgcgccacag cagcaatatc agcagccgca acagccggtt gcgccgcagc cacaatatca
    17701 gcagccgcaa caaccggttg cgccacagca gcaatatcag cagccgcaac aaccagttgc
    17761 gccgcagccg cagtatcaac agccgcaaca accggttgcg ccgcagcagc agtatcagca
    17821 gccacaacag ccagttgcgc cacaaccgca ggataccctg cttcatccgc tgttgatgcg
    17881 taatggcgac agccgtccgt tgcataaacc gacgacgccg ctgccttctc tggatttgct
    17941 gacaccgccg ccgagcgaag tggagccggt agataccttt gcgcttgaac aaatggctcg
    18001 cctggtggaa gcgcgtctgg ctgatttccg tattaaagcc gatgttgtca attactctcc
    18061 ggggccggtt atcactcgct ttgaattgaa cctggcaccg ggcgtaaaag cggcgcgcat
    18121 ttctaacttg tcacgggacc ttgcccgttc actttcgacg gtggcggtgc gtgtcgttga
    18181 agttattcct ggcaaaccct atgtaggtct ggagttaccg aataaaaaac gacaaaccgt
    18241 ttatctgcgc gaagttttgg ataacgccaa attccgcgat aatccgtcgc cattaaccgt
    18301 ggtgctgggt aaagatatcg ccggtgagcc ggtggttgcc gatctggcga aaatgccgca
    18361 cttgttggtt gcggggacta ccggttccgg taaatctgtc ggtgtgaacg cgatgatcct
    18421 gagcatgctt tataaagcac agccagaaga tgtgcgtttc atcatgatcg acccgaaaat
    18481 gctggagctt tcggtttatg aaggcattcc gcatctgtta acggaagtcg ttactgatat
    18541 gaaagatgcc gccaacgcgc tgcgctggtg tgttaacgag atggagcgtc gttataaact
    18601 gatgtctgcg ctgggtgtgc gtaatctggc gggttataac gaaaaaattg ctgaagccga
    18661 tcgcatgatg cgtccgattc cagacccgta ctggaagccg ggtgacagta tggatgccca
    18721 gcatccggtg ctgaaaaaag aaccatacat tgtggtgttg gttgacgaat ttgccgacct
    18781 gatgatgacg gtaggtaaaa aagtggaaga gctgatagca cgtctggcgc aaaaagcccg
    18841 tgccgcgggt atccacctcg tactggcaac tcagcgtcca tcggttgatg ttattactgg
    18901 tctgattaaa gcgaatattc cgacccgtat cgcctttacc gtatccagta agattgactc
    18961 acgtaccatt cttgatcagg ctggcgcgga atcactgctg gggatggggg atatgctcta
    19021 ctctgggccg aactccacgt tgccggtacg tgtccatggt gcttttgttc gcgatcagga
    19081 agttcatgcc gtggtgcagg actggaaagc gcgtggtcgc ccacagtatg ttgatggcat
    19141 cacttccgac agtgaaagcg aaggtggcgc gggcggtttc gatggcgctg aagaactgga
    19201 tccgttgttc gatcaggcgg tgcagtttgt cactgaaaaa cgcaaagcgt caatttctgg
    19261 cgtacagcgt cagttccgca ttggttataa ccgtgcagcg cgtattatcg aacagatgga
    19321 agcgcagggg attgtcagcg aacaggggca caacggtaat cgtgaagtgc tggctccacc
    19381 gccgtttgac taattaatgc attgccggat aaggcgcggt agcgtcgcat ccggcactct
    19441 atcaactgaa aattcagtat tttcttcttt cctcaagctg attattagcc tggaatagag
    19501 agtagaggga actcccgatc gggagtgacg taatttgagg aataatgatg aaaaaaattg
    19561 ccatcacctg tgcattactc tcaagcttag tagcaagcag cgtttgggct gatgccgcaa
    19621 gcgatctgaa aagccgcctg gataaagtca gcagcttcca cgccagcttc acacaaaaag
    19681 tgactgacgg tagcggcgcg gcggtgcagg aaggtcaggg cgatctgtgg gtgaaacgtc
    19741 caaacttatt caactggcat atgacacaac ctgatgaaag cattctggtt tctgacggta
    19801 aaacactgtg gttctataac ccgttcgttg agcaagctac ggcaacttgg ctgaaagatg
    19861 ccaccggtaa tacgccgttt atgctgattg cccgcaacca gtccagcgac tggcagcagt
    19921 acaatatcaa acagaatggc gatgactttg tcctgacgcc gaaagccagc aatggcaatc
    19981 tgaagcagtt caccattaac gtgggacgtg atggcacaat ccatcagttt agcgcggtgg
    20041 agcaggacga tcagcgcagc agttatcaac tgaaatccca gcaaaatggg gctgtggatg
    20101 cagcgaaatt taccttcacc ccgccgcaag gcgtcacggt agatgatcaa cgtaagtaga
    20161 ggcacctgag tgagcaatct gtcgctcgat ttttcggata atacttttca acctctggcc
    20221 gcgcgtatgc ggccagaaaa tttagcacag tatatcggcc agcaacattt gctggctgcg
    20281 gggaagccgt tgccgcgcgc tatcgaagcc gggcatctgc attctatgat cctctggggg
    20341 ccaccgggta ccggcaaaac aaccctcgct gaagtgattg cccgctatgc gaacgctgat
    20401 gtggaacgta tttctgccgt cacctctggc gtgaaagaga ttcgcgaggc gatcgagcgc
    20461 gcccggcaaa accgcaatgc aggtcgccgc actattcttt ttgttgacga agtgcaccga
    20521 ttcaacaaaa gccagcagga tgcatttctg ccacatattg aagacggcac catcactttt
    20581 attggcgcaa ccactgaaaa cccgtcgttt gagcttaatt cggcactgct ttcccgtgcc
    20641 cgtgtctatc tgttgaaatc cctgggtaca gaggatattg agcaagtact aactcaggcg
    20701 atggaagaca agacccgtgg ttatggtggt caggatattg ttctgccaga tgaaacacga
    20761 cgcgccattg ctgaactggt gaatggcgac gcgcgccggg cgttaaatac gctggaaatg
    20821 atggcggata tggccgaagt caatgatagc ggtaagcggg tcctgaagcc tgaattattg
    20881 accgaaatcg ccggtgagcg aagcgcccgc tttgataaca aaggcgatcg cttttacgat
    20941 ctcatttccg cactgcataa gtcggtacgt ggtagcgcac ccgatgcggc gctctactgg
    21001 tatgcgcgaa ttattaccgc tggtggcgat ccgttatatg tcgcgcgtcg ttgcctggca
    21061 attgcttccg aagatgtcgg caatgccgac ccacgggcga tgcaggtggc aattgcggcc
    21121 tgggattgct ttactcgcgt tggcccggcg gaaggtgaac gcgccattgc tcaggcgatt
    21181 gtttacctgg cctgcgcgcc aaaaagcaac gctgtctaca ctgcgtttaa agctgcgctg
    21241 gcggatgctc gcgaacgccc ggattatgac gtgccggttc atttgcgtaa tgcgccgacg
    21301 aaattaatga aggaaatggg ctacgggcag gaatatcgtt acgctcatga tgaagcaaac
    21361 gcttatgctg ccggtgaggt ttacttcccg ccggaaatag cacaaacacg ctattatttc
    21421 ccgacaaaca ggggccttga aggcaagatt ggcgaaaagc tcgcctggct ggctgaacag
    21481 gatcaaaata gccccataaa acgctaccgt taatgttatc gttgcggtaa tgttgttact
    21541 gtatccctgt ggtcgcaggc tgtggccaca tctcccattt aattcgataa gcacaggata
    21601 agcatgctcg atcccaatct gctgcgtaat gagccagacg cagtcgctga aaaactggca
    21661 cgccggggct ttaagctgga tgtagataag ctgggcgctc ttgaagagcg tcgtaaagta
    21721 ttgcaggtca aaacggaaaa cctgcaagcg gagcgtaact cccgatcgaa atccattggc
    21781 caggcgaaag cgcgcgggga agatatcgag cctttacgtc tggaagtgaa caaactgggc
    21841 gaagagctgg atgcagcaaa agccgagctg gatgctttac aggctgaaat tcgcgatatc
    21901 gcgctgacca tccctaacct gcctgcagat gaagtgccgg taggtaaaga cgaaaatgac
    21961 aacgttgaag tcagccgctg gggtaccccg cgtgagtttg actttgaagt tcgtgaccac
    22021 gtgacgctgg gtgaaatgca ctctggcctc gactttgcag ctgcagttaa gctgactggt
    22081 tcccgctttg tggtaatgaa agggcagatt gctcgcatgc accgcgcact gtcgcagttt
    22141 atgctggatc tgcataccga acagcatggc tacagtgaga actatgttcc gtacctggtt
    22201 aaccaggaca cgctgtacgg tacgggtcag ctgccgaaat ttgctggcga tctgttccat
    22261 actcgtccgc tggaagaaga agcagacacc agtaactatg cgctgatccc aacggcagaa
    22321 gttccgctga ctaacctggt gcgcggtgaa atcatcgatg aagatgatct gccaattaag
    22381 atgaccgccc acaccccatg cttccgttct gaagccggtt catatggtcg tgacacccgt
    22441 ggtctgatcc gtatgcacca gttcgacaaa gttgaaatgg tgcagatcgt gcgcccggaa
    22501 gactcaatgg cggcgctgga agagatgact ggtcatgcag aaaaagtcct gcagttgctg
    22561 ggcctgccgt accgtaaaat catcctttgc actggcgaca tgggctttgg cgcttgcaaa
    22621 acttacgacc tggaagtatg gatcccggca cagaacacct accgtgagat ctcttcctgc
    22681 tccaacgttt gggatttcca ggcacgtcgt atgcaggcac gttgccgcag caagtcggac
    22741 aagaaaaccc gtctggttca taccctgaac ggttctggtc tggctgttgg tcgtacgctg
    22801 gttgcagtaa tggaaaacta tcagcaggct gatggtcgta ttgaagtacc agaagttctg
    22861 cgtccgtata tgaacggact ggaatatatt ggctaatacc caatttttct gaatctaaaa
    22921 agcgcctgcg ggcgcttttt ttgtctccct ttgataccga acaataatta ctcttcactt
    22981 acacgtaata ctactttcga gtgaaaatct acctatctct ttgattttca aattattcga
    23041 tgtatacaag cctatatagc gaactgctat agaaataatt acacaatacg gtttgttact
    23101 ggaatcaatc gtgagcaagc ttgagtgagc cattatgaaa acgaaaatcc ctgatgcggt
    23161 attggctgct gaggtgagtc gccgtggttt ggtaaaaacg acagcgatcg gcggcctggc
    23221 aatggccagc agcgcattaa cattaccttt tagtcggatt gcgcacgctg tcgatagcgc
    23281 cattccaaca aaatcagacg aaaaggttat ctggagcgcc tgtacagtta actgtggtag
    23341 tcgctgcccg ctacgtatgc acgtcgtgga cggtgaaatc aaatatgtcg aaacggacaa
    23401 taccggcgat gacaattacg acggcctgca ccaggttcgc gcctgcctgc gtgggcgttc
    23461 catgcgtcgc cgtgtctaca atccggaccg cctgaaatat ccgatgaaac gagtcggggc
    23521 gcgcggtgaa ggcaaattcg agcgcattag ctgggaagaa gcctacgaca tcatcgcgac
    23581 caatatgcag cgcctgatca aagagtacgg caacgagtct atctatctga actatggcac
    23641 cggtacgctg ggcggcacca tgacccgctc ctggccgccg ggaaataccc tggtcgcgcg
    23701 gctgatgaac tgctgcggcg gctatctgaa ccattacggc gactactcct ccgcgcaaat
    23761 tgcggaaggt ttgaactata cctacggcgg ctgggcagat ggcaacagcc cgtcggatat
    23821 cgaaaacagt aagctggtag tgctgtttgg taataaccct ggcgaaacgc gaatgagtgg
    23881 cggtggggtg acttactatc ttgaacaggc acgccagaaa tctaatgccc gcatgatcat
    23941 catcgatccg cgctataccg acaccggtgc cgggcgcgaa gatgagtgga tccctattcg
    24001 tccgggaaca gatgccgcac tggttaacgg tctggcgtac gtcatgatca ctgaaaacct
    24061 ggtggatcag gcattcctcg ataaatattg cgttggctac gatgagaaaa ccctgccagc
    24121 cagtgcgccg aaaaatggcc actataaagc ttatattctg ggtgaagggc cagatggcgt
    24181 ggctaaaaca ccggaatggg cctcacaaat caccggtgtt ccggcagaca aaatcatcaa
    24241 actggctcgt gaaatcggca gtaccaaacc ggcgtttatc agccagggat ggggcccgca
    24301 gcgtcacgct aacggtgaaa tcgcaacccg tgctatctcg atgctggcga ttctgaccgg
    24361 taacgttggt attaatggag ggaacagcgg cgcgcgtgaa ggttcataca gcttaccgtt
    24421 tgtccgtatg ccgaccttgg aaaacccgat ccagaccagc atttcgatgt ttatgtggac
    24481 cgatgccatt gaacgtggcc cggaaatgac ggcgctgcgt gatggtgtac gcgggaaaga
    24541 taagctggat gtgccgatca aaatgatctg gaactatgcc ggtaactgcc tggttaacca
    24601 gcattctgaa atcaaccgta cccatgaaat ccttcaggat gataagaagt gcgagctgat
    24661 tgtggttatc gactgccaca tgacctcatc ggcgaaatat gctgacatcc tgctgcctga
    24721 ctgcaccgct tccgaacaga tggactttgc gctggatgca tcctgcggga atatgtctta
    24781 cgtgattttc aacgatcagg tgattaaacc gcgctttgaa tgtaagacca tctatgaaat
    24841 gaccagcgaa ctggcaaaac gtcttggcgt tgagcaacag tttactgaag gccgtaccca
    24901 ggaagagtgg atgcggcatc tgtatgccca gtcgcgggaa gcgattcctg aactgccaac
    24961 gtttgaagag ttccgcaagc aggggatctt taaaaagcgc gacccacaag ggcatcacgt
    25021 tgcttataaa gccttccgtg aagatccgca ggcaaaccca ctgactacgc catcgggcaa
    25081 aattgagatt tattcgcagg cgctggctga cattgccgct acctgggaat tgccagaagg
    25141 cgatgtgatc gatccactgc cgatctacac gccgggcttt gaaagttatc aggatccgct
    25201 gaacaaacag tatccgctgc agcttacggg tttccactat aaatctcgcg ttcactcaac
    25261 ttacggcaac gttgatgtgc tgaaagcagc ttgccgtcag gaaatgtgga tcaacccgct
    25321 tgatgcgcaa aaacgcggta tcaacaacgg cgataaagtc aggatcttta acgatcgtgg
    25381 tgaagttcat attgaggcga aagtgacgcc acgaatgatg ccaggtgtgg tcgcactggg
    25441 tgaaggtgcc tggtatgacc cggatgcaaa acgtgtcgat aagggtggtt gtattaacgt
    25501 actgaccact caacgtccgt ctcctctcgc taaggggaat ccgtcacata caaaccttgt
    25561 tcaggttgaa aaggtgtaag gagtaaccga tgacaaccca gtatggattt tttattgatt
    25621 ccagccgttg caccggttgc aaaacctgcg agctggcttg taaagactac aaagatttga
    25681 cgccagaagt cagcttccgc cgcatttatg aatatgctgg cggcgactgg caggaagata
    25741 acggtgtctg gcaccagaac gtgtttgcct actatctgtc gatttcgtgc aaccactgcg
    25801 aagatccggc ttgtactaaa gtctgcccga gcggtgcgat gcataaacgt gaagatggtt
    25861 ttgttgtggt cgatgaagat gtgtgcattg gctgccgcta ctgccatatg gcttgcccgt
    25921 atggcgcacc gcaatataac gaaacgaaag gccatatgac caaatgcgat ggttgttatg
    25981 accgtgttgc ggagggtaaa aagccgatct gtgttgaatc ttgtccgctg cgggcgctgg
    26041 atttcggccc tatcgacgag ctgcgtaaaa aacatggcga tctggcggcc gttgcgccgt
    26101 tgccgcgagc tcactttacc aaaccgaata ttgtgatcaa acccaatgcc aatagccgcc
    26161 cgaccgggga taccactggc tatctggcaa acccgaagga ggtgtgagat gggaagtgga
    26221 tggcatgaat ggccgctgat gatcttcacg gtcttcgggc aatgtgtggc gggtggtttt
    26281 atcgttctgg ctttggcgct gctcaaaggc gacctgcgag cagaagccca gcagcgtgtt
    26341 atcgcctgca tgtttggctt atgggtgctg atgggcattg gctttatcgc ttctatgctc
    26401 catcttggtt caccgatgcg cgcttttaac tcgctcaacc gggtaggggc ttcagcactc
    26461 agtaacgaaa tcgccagcgg ttcgatcttt tttgccgtag gcggcatcgg ctggttgctg
    26521 gcaatgctga aaaagctgtc accggcattg cgtacgcttt ggctggtggt gacgatggtt
    26581 cttggcgtcg tctttgtctg gatgatggtg cgtgtgtata acagcattga taccgttccg
    26641 acctggtaca gcatctggac gccgatgggc ttcttcctga cgatgttcat gggcggcccg
    26701 ttactggggt acctgttgct gagtctggct ggcgtcgata gctgggcgat gcgtctgctg
    26761 ccagccattt ccgtactggc actggtggtg agtggcgtgg tgtcagtgat gcagggcgca
    26821 gagctggcga ctattcatag ttctgtgcag caggccgcag cgctggtgcc ggactatggt
    26881 gcgctgatgt cctggcggat cgtgcttttg gccgttgccc tgtgcttgtg gattgcacca
    26941 cagctaaaag gttatcagcc tgcggttccg ctactatccg tctcattcat tctgcttctg
    27001 gcaggggaat taatcggtcg cggcgtattc tatggtttgc atatgaccgt ggggatggcc
    27061 gtcgcaagct aatcagtaac aaccggggtt tcggccccgg ttctctttta tttctgcttc
    27121 gttaacgtgt cataactggt catcaaatta cgataatcag gaatatggtt agagaacaac
    27181 gtcgccagtc cttcaatatc attacgccag tcacggtgca gctcgcaagc cacgccgaac
    27241 catgtcatca attgcgcacc tgcttgcgac atgcgatccc atgccgaatg ccgggtaatt
    27301 tcattaaagg tccctgacgc gtcagtcaca acaaacacat caaacccttc ttcaatggct
    27361 gaaagcgccg ggaatgccac gcaaacttcg gttaccacac cggcaataat taactgtttt
    27421 ttacctgtcg ctttgactgc ttttacaaaa tcttcgttat cccaggcgtt aatattgccc
    27481 gggcgagcaa tgtaaggtgc atcggggaat tgtgctttca gttctggaac taatgggccg
    27541 ttaggaccgg tttcaaaact ggtggtaaga atagttggta agttgaaata cttagccagg
    27601 tcacccagcg ccagcacgtt atttttaaac ttatcgggtt cgatatcccg tacaagggaa
    27661 agtaaaccag cctggtgatc aacaagcaaa acagcagcat catttttatc aagacgaaca
    27721 tacggtttgg tcatcctctt ctcctttcga gagtcagtgg tacgcctgaa atcggtcaga
    27781 cgtgatgcat tgctctgaaa gcatagacgg gaaatatgag tttgctgtga ccatgaaatt
    27841 tttcgactga acgaagcgta tgaaatttgt gttagatcaa taaaaacaat cagatgagat
    27901 taatcatctc ttcagtatta gcgttatgaa tgagtaatat aaatcgataa taccgccgtt
    27961 gcgatatgcg cgtattttca cccattgaca atgtttttgg cggtggcatg atgcgcatga
    28021 aatttgaact tcctcacggt tttaattcat gtccacgtat acccggcctg tcatgctttt
    28081 gctgtctggc ctgcttttgt tgactctggc gatcgcggtg ttaaatacac tcgtgccgct
    28141 ttggctcgcc caggaacaca tgtccacatg gcaggtaggc gttgtcagct catcctattt
    28201 taccggcaac cttgtcggta cattgctgac agggtatgtc attaaacgca ttggttttaa
    28261 ccgcagctat tatctggcct ccttcatttt tgccgctggc tgtgccggcc ttggcctgat
    28321 gattggattc tggagttggt tggcgtggcg ttttgtcgcg ggcgtcggct gtgccatgat
    28381 ttgggtagtc gttgagagcg cgctgatgtg cagtgggacg tcacgtaacc gtgggcgttt
    28441 gcttgctgca tacatgatgg tttattacgt gggaacgttt ttaggccagt tactggtcag
    28501 caaggtctca accgagctga tgagcgtatt gccgtgggtt acaggtttga cattggcagg
    28561 gatcttaccg ctgttgttta cgcgtgtcct gaatcagcag gctgaaaacc atgattcgac
    28621 gtcaattacg gcaatgctaa aactccgtca ggcgcggctt ggcgtgaatg gctgcattat
    28681 ctcaggaatc gttctgggat ctctatatgg cctgatgcct ctgtacctca atcacaaagg
    28741 ggtgagcaat gccagtattg gtttctggat ggcggtactg gtcagtgcgg gtatcctcgg
    28801 acaatggccg attggacgtc tggcggataa gtttggtcga ctgctggttt tgcgtgttca
    28861 ggtctttgtc gtcattctcg gcagtattgc gatgcttagc caggcggcga tggccccagc
    28921 gttattcatc ctcggtgccg ctggctttac gctatatccg gtggcgatgg catgggcttg
    28981 cgagaaagtt gaacatcatc aactggtggc gatgaaccaa gccttactgt tgagctatac
    29041 tgtgggaagt ctgcttggcc cgtcatttac cgctatgcta atgcagaatt tctccgataa
    29101 tttattgttt atcatgatcg ccagcgtatc gtttatctat ttgctgatgc tgctgcgcaa
    29161 cgccggtcat acgccgaaac ccgttgctca cgtgtaaatg aattcaagca gagtgtgaac
    29221 ttactgtttc acactctgct tttttgtttc ttctatctga cttgctttat tccaaatttt
    29281 attcgtttaa aaataaaatg tgtagcaggt tataattttg catttcgcta tttccgcact
    29341 tcttatttgc cgcgcataat ccctcgtttt acagatgccc ctataatttt ggcgaaggat
    29401 ttgtctatgg ctgggaatgt tcaggaaaaa cagctgcgat ggtacaacat tgcgctgatg
    29461 tcttttatca ctgtctgggg ttttggcaac gttgttaata actacgccaa ccaggggctg
    29521 gtggttgttt tttcatgggt gtttatcttt gcgctctatt tcacacctta tgcgctaatt
    29581 gttggtcagt taggctcgac cttcaaagat gggaagggcg gggtcagtac ctggattaaa
    29641 cacacgatgg gacccggact ggcttatctc gccgcgtgga cctactgggt ggtgcatatt
    29701 ccctatctgg cacaaaaacc ctaggcaatt ctgattgcgc tcggttgggc gatgaaaggc
    29761 gacggttcgc taatcaaaga atattcagtc gtagcgttac aggggttaac gctggtgctg
    29821 tttatcttct ttatgtgggt tgcttcacgc ggtatgaaat cgctgaaaat cgtcggttct
    29881 gtggcaggga ttgctatgtt tgttatgtcg ctcctgtatg tggcgatggc ggtaaccgcg
    29941 cctgcaatta ctgaagtgca tattgcgacc acaaacatta cctgggaaac gttcattcct
    30001 catatcgact ttacctacat taccactatt tcaatgctgg ttttcgcggt tggcggagca
    30061 gagaagattt ctccttacgt taatcaaacg cgcaacccag gaaaagaatt tccaaaaggg
    30121 atgttatgcc tggcggtgat ggttgcggtt tgtgccattc tgggctcgct ggcgatgggg
    30181 atgatgtttg attcgcgtaa tatcccggat gacttaatga ccaacggtca gtattacgcc
    30241 tttcagaagc tgggcgagta ttacaacatg ggtaatactt taatggtgat ttacgccatt
    30301 gcgaataccc tgggacaagt agcggcgctg gtattctcga ttgatgcccc gcttaaagtg
    30361 ctattaggtg atgctgacag caaatatatt ccagccagtt tatgtcgtac caacgcttct
    30421 ggtacgcccg ttaatggcta ttttctgacc ctggtactgg tggcgattct gattatgctg
    30481 ccgactctcg gcattggtga tatgaacaat ctctataaat ggctgttgaa ccttaattcg
    30541 gtagtgatgc cgctgcgtta tttatgggta tttgttgcat ttattgcagt cgttcgcctg
    30601 gcgcagaaat ataaaccaga atatgtcttt attcgtaata agcctctggc aatgaccgtc
    30661 gggatttggt gttttgcctt taccgccttt gcctgtttga cggggatctt cccgaaaatg
    30721 gaagccttca ctgcagagtg gaccttccag ttggcgctga atgttgcaac gccgtttgtg
    30781 ctggtaggat taggactgat attcccgctg ctggcgcgta aagcgaatag taaataatta
    30841 ttgtggtcgg tgacgctgac tctggcagat ctgacccgtt tttacataat cgagaaatgc
    30901 gcgcaagccg caggacatat actggcggtt tgggtaatag atctggaagc ctggacgctc
    30961 tgtgatccag tcttccagta cacattctaa acgaccggta tcaaggtact ccttgatctg
    31021 ttcatagagc agatacccga taccagcacc catcaaaacg gcatccagtt cagcatcaac
    31081 atca
//
LOCUS       DAJTZL010000031        30963 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.031, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000031 DAJTZL010000000
VERSION     DAJTZL010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30963
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.031"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..642)
                     /locus_tag="OO544_001248"
     CDS             complement(<1..642)
                     /locus_tag="OO544_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE9 sensor domain-containing protein"
                     /protein_id="HCR6766885.1"
                     /translation="MVKKPQQGTIFAAAQRSDSYFVKCINMLSLYEKIKIRLIILFLL
                     AALSFIGLFFIINYQLVSERAVKRADSRFELIQKNVGYFFKDIERSALTLKDSLYLLK
                     NTEEIQRAVILKMEMMPFLDSVGLVLDDNKYYLFSRRANDKIVVYHQEQVNGPLVDES
                     GRVIFADFNPSKRPWSVASDDSNNSWNPAYNCFDRPGKKCISFTLRINGKDHDL"
     gene            complement(742..1653)
                     /gene="gsiD"
                     /locus_tag="OO544_001249"
     CDS             complement(742..1653)
                     /gene="gsiD"
                     /locus_tag="OO544_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HCR6766886.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(1656..2576)
                     /gene="gsiC"
                     /locus_tag="OO544_001250"
     CDS             complement(1656..2576)
                     /gene="gsiC"
                     /locus_tag="OO544_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HCR6766887.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGLEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(2594..4132)
                     /gene="gsiB"
                     /locus_tag="OO544_001251"
     CDS             complement(2594..4132)
                     /gene="gsiB"
                     /locus_tag="OO544_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HCR6766888.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAVAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(4152..6023)
                     /gene="gsiA"
                     /locus_tag="OO544_001252"
     CDS             complement(4152..6023)
                     /gene="gsiA"
                     /locus_tag="OO544_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HCR6766889.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLQRRSREVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQAPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(6010..6975)
                     /gene="iaaA"
                     /locus_tag="OO544_001253"
     CDS             complement(6010..6975)
                     /gene="iaaA"
                     /locus_tag="OO544_001253"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCR6766890.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFARGMERVSPEIFSTPLRYEQLLAARKEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCIGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            7179..8414
                     /gene="moeA"
                     /locus_tag="OO544_001254"
     CDS             7179..8414
                     /gene="moeA"
                     /locus_tag="OO544_001254"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HCR6766891.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVGEWVEVEPFNALFGGL"
     gene            8414..9163
                     /gene="moeB"
                     /locus_tag="OO544_001255"
     CDS             8414..9163
                     /gene="moeB"
                     /locus_tag="OO544_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HCR6766892.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLIVGLGGLGC
                     AASLYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     TSGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(9342..10004)
                     /gene="fsa"
                     /locus_tag="OO544_001256"
     CDS             complement(9342..10004)
                     /gene="fsa"
                     /locus_tag="OO544_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HCR6766893.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVAKFEQDWQGAFGRT
                     SI"
     gene            10135..11034
                     /locus_tag="OO544_001257"
     CDS             10135..11034
                     /locus_tag="OO544_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HCR6766894.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMTTVLRDKPFYDRSGGGLTLSGGEPFMQPEMAMALLQASHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            11040..13472
                     /locus_tag="OO544_001258"
     CDS             11040..13472
                     /locus_tag="OO544_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HCR6766895.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALAREMAATETRESRRDELLAIAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQTLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            13618..14433
                     /gene="ybiV"
                     /locus_tag="OO544_001259"
     CDS             13618..14433
                     /gene="ybiV"
                     /locus_tag="OO544_001259"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HCR6766896.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKAVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            14585..15850
                     /locus_tag="OO544_001260"
     CDS             14585..15850
                     /locus_tag="OO544_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003570920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HCR6766897.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLDRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRRPPGTTSKGLGAHTDSGALERWLLPAYQ
                     RVFANVFNGNLAQYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSNMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGIDS"
     gene            complement(15817..16977)
                     /locus_tag="OO544_001261"
     CDS             complement(15817..16977)
                     /locus_tag="OO544_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000505360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR6766898.1"
                     /translation="MGFFAHENSVISELEADHKMDKKLKIADIAARTGLSPSTVSRVL
                     AGKANTSARAREQVLACARELGVMDGMAAGRMLLNNLIIFAPQRAFDQRSDIFYYRVI
                     QSISKALSPHEVRLRYCALEEFDSDPALFLSRMNEADTQAAILLGIDDPHIHDLAADL
                     AKPCVLINCRDERMRLPAIAPDHRLIGAFAARYLFEMGHREVMNVMCLRRYTMEQRFA
                     GIKESWRQHNLAFHHDRHLLNISSFSAKEAEEKVGAWLDAANGKTLPTAFLVGGDFMA
                     AGTVNALHRRGLRVPQDVSVMSIDGFNLAAIEDVPLTAVHVPRDELGNEAVQMLQQRL
                     IRPDAPVGSLLLHGRLAVRESVRRVRPGKGNTAVEREGLYDTQLSIPNARLP"
     gene            17215..18516
                     /locus_tag="OO544_001262"
     CDS             17215..18516
                     /locus_tag="OO544_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6766899.1"
                     /translation="MSQGINNDMTASKPRHIVKNLRWWILVLFLLGVTVNYITRNSLG
                     ILAPELKSSLGITTEQYSWIVGAFQLAYTLFQPLCGWLIDVIGLKLGFMICAGLWALA
                     CLMHAGAGNWGHLALLRFFMGGAEAAATPANAKTIGEWFPKSERPIAAGWAGVGFSIG
                     AMLAPPIIYFAHASFGWQGAFMFTGILAMLWVVLWWAFYHSPDKHPNLSKAELDFIRQ
                     DNEAPPVKLPFLTALKTVGKNKRFYGIAIPAFMAEPAWAVMSFWVPLYLSKTYGMDLK
                     QIALFAWLPFLAADLGSIASGYLTKLYTRLFGLSRVNSVVASSVSGAFLMVSLAVMAF
                     TNNPYIAIVLISIGGFGHQIISCMLSALVVESFDKGQMATVNGMRGSAAWIASFLFSL
                     IIGVTADKIGFNPLFIAMGFFDLIGAFFLVTFIAERRAKRA"
     gene            18531..20894
                     /locus_tag="OO544_001263"
     CDS             18531..20894
                     /locus_tag="OO544_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HCR6766900.1"
                     /translation="MKTLKNWTLHKQSAHHVELLVDGQHILCLYVLEENLFRVLLKRK
                     GELALDRTWSIAPAQDVPWEGRSREDLSGFSLPAWTVIQHDDTVVIATESLRATVHQP
                     LWLEWHYRNNEGEWLPLVNDRPTSAYLLNAHGDGVAHYQSRRKDERFYGLGDKAGDLQ
                     RNGKRYEMRNLDAMGYNAVSTDPLYKHIPFTITRRDDISFGLFYDNLSSCWLDLGNEI
                     DNYHTAYRRWQAEAGDIDYYLFTGERVLDITKAFVRLTGKTLFGPKWSLGYSGSTMHY
                     TDAPDAQNQLMNFIRLCEEHAIPCDSFQLSSGYTSINGKRYVFNWNYDKVPQPKVMSQ
                     AFHNAGLHLAANIKPCLLQDHPRYNEVAERGLFIRDSETDEPERSSFWDDEGSNLEFT
                     NPQTIQWWQEGVTTQLLEMGIDSTWNDNNEFEVWDGEARCHGFGQTIAIKHIRPVMPL
                     LMMRASMEAQQRFAPEKRPYLISRSGCAGMQRYVQTWSGDNRTSWDTLRYNTRMGLGM
                     SLSGLYNLGHDVGGFSGDKPDPELFVRWVQNGVMHPRFTIHSWNDDHTVNEPWMYPGV
                     TPAIRSAIELRYRLLPYFYTLMWQAYADDEPMLRPTFLDHEHDVQTFEECDDFLLGRD
                     ILVASVVEQGERQRRVWLPDNETGWYDFYNGEWFSGGQWITLDAPLEKLPLMVRAGAG
                     LPLSKRITYVSAEQDDTRELKLFPLKGVGTTSGLLFEDDGESWGYQTGNALWVEWEMV
                     CDGATINLKVNARGDYRPAWSALKVSLPLEEKRTLLVNGVEGSEWMR"
     gene            complement(21074..22669)
                     /locus_tag="OO544_001264"
     CDS             complement(21074..22669)
                     /locus_tag="OO544_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HCR6766901.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPEILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERAIDFSGNYEDYLRSKGIDA"
     gene            22888..23808
                     /gene="ldtB"
                     /locus_tag="OO544_001265"
     CDS             22888..23808
                     /gene="ldtB"
                     /locus_tag="OO544_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR6766902.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(23867..24985)
                     /locus_tag="OO544_001266"
     CDS             complement(23867..24985)
                     /locus_tag="OO544_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HCR6766903.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWSCLGLYIVFLTALE
                     FKQELWGLVIVAAGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLVWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVILPAN"
     gene            complement(24982..25449)
                     /gene="mntR"
                     /locus_tag="OO544_001267"
     CDS             complement(24982..25449)
                     /gene="mntR"
                     /locus_tag="OO544_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HCR6766904.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            25635..25763
                     /gene="mntS"
                     /locus_tag="OO544_001268"
     CDS             25635..25763
                     /gene="mntS"
                     /locus_tag="OO544_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HCR6766905.1"
                     /translation="MNEFKKCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            26035..27618
                     /gene="opgE"
                     /locus_tag="OO544_001269"
     CDS             26035..27618
                     /gene="opgE"
                     /locus_tag="OO544_001269"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HCR6766906.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(27667..28182)
                     /gene="ompX"
                     /locus_tag="OO544_001270"
     CDS             complement(27667..28182)
                     /gene="ompX"
                     /locus_tag="OO544_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HCR6766907.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(28235..28300)
                     /locus_tag="OO544_001271"
     CDS             complement(28235..28300)
                     /locus_tag="OO544_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6766908.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            28535..29422
                     /gene="rhtA"
                     /locus_tag="OO544_001272"
     CDS             28535..29422
                     /gene="rhtA"
                     /locus_tag="OO544_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HCR6766909.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            29721..30224
                     /gene="dps"
                     /locus_tag="OO544_001273"
                     /gene_synonym="pexB"
     CDS             29721..30224
                     /gene="dps"
                     /locus_tag="OO544_001273"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HCR6766910.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            30628..>30963
                     /locus_tag="OO544_001274"
     CDS             30628..>30963
                     /locus_tag="OO544_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HCR6766911.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGL"
BASE COUNT         7560 a         8415 c         7718 g         7270 t
ORIGIN      
        1 caaatcgtga tctttgccgt tgatacgtag cgtaaaagag atacattttt tacccggacg
       61 atcaaagcaa ttgtatgccg gattccagct gttgttagag tcatctgaag ccaccgacca
      121 cggtcgtttc gatgggttaa aatcggcaaa aataacccgc cctgactcgt cgacaagcgg
      181 tccatttact tgttcctgat gataaacaac gattttatca ttcgccctcc gcgaaaagag
      241 ataatattta ttatcatcaa gtaccagtcc caccgagtct aaaaatggca tcatttccat
      301 tttcagaatc acggcgcgtt gaatctcctc tgtatttttt aataaataca gtgagtcctt
      361 taatgtcagg gccgaacgtt caatatcttt aaagaaatag ccaacgtttt tctgaataag
      421 ttcaaagcgg ctatcggcac gttttaccgc gcgttccgat accagttgat agttaatgat
      481 gaaaaaaaga ccaataaatg acagtgctgc caataaaaat aaaattatca gccttatctt
      541 tatcttttcg tataaactca gcatgttaat acatttaaca aaataactat ctgaacgctg
      601 tgccgctgca aaaatagtcc cttgctgcgg ctttttaacc aaattacccg attctacaaa
      661 atcagcagaa agttacaaca cttgcttagg gcaataattt cctgtgaggt gattaccctt
      721 tcaagcaata ttcaaacgta actatccttt aattttcgga tccagcgcat cgcgtaaacc
      781 atcgcccaac aaattgaacg ccagtacggt cagaaaaata gccagggccg gaaaaacagc
      841 gacatgcggc gcgataacca tatccgctcg tgcctcattg agcattgctc cccactctgg
      901 tgtcggcggc tgcgcaccga ggccgagaaa tgacaggctg gcggcagaga taatcgaggt
      961 gccaatgcgc atggtgaaaa acaccacgat agacgagacg gtcccaggca agatatgacg
     1021 caataaaatg gtcatatcgc tggcaccaat actgcgtgct gactcaataa aggtttgctg
     1081 tttcaacacc agcgtgttgc cgcgcaccag tcgggcaaac gcggggatgg aaaaaatggc
     1141 gactgcgata atcacgttag caatgccgct tcccaacacc gcaacaacag cgatcgccag
     1201 taaaataccc gggaaggcaa acagcacatc gcaaatgcgc atgatcagcc gatcccacca
     1261 gccttcataa tatccagcca acaagcccag caacgtcccg atcgccgcac cgataaacac
     1321 ggcaaacacg cccgccgcca gcgagatttg cgcaccaacc aggacacggc tgaaaatgtc
     1381 gcgccccagt gaatcgacgc caaaccagtg ctgcaaagaa ggtccgttat tcagattgtc
     1441 ataatcaaaa taattttcgg cgtcataggg agcgatccag cgtgcaaaaa tggccaccac
     1501 aatcaataaa ataacgaata atgcggcggt catcgccata tgctgacggc gaaatcgtcg
     1561 ccagaattca tgccacggtg tacgcacctg gtcaggtttg accagtggca tggcgtttaa
     1621 caccgcctga cgtcgccagt taaatagtcg catccttact tgtacctgat agccgggtta
     1681 atggcggcgt aaagcacatc caccactaag ttgataagaa taaattccag cgagaaaagc
     1741 agaatttccg cctgaatcac cgggtaatcg cgcatttcta cggagtcaac gagtaagcgt
     1801 ccaagtccgg gccagttgaa aactttctcc accacgatag aaccaccgag caaaaagcca
     1861 aactgtaagc ccatcatggt cactaccggg atcatcgcgt tacgtagccc gtgtttgagg
     1921 acaacccagg tttcgctcac ccctttcgcc ctcgcggtac gcatataatc ttcgcttaaa
     1981 acatcgacaa acgacgcgcg ggtaaagcgc gccatcacgg ctgccactgc cgcgccgagc
     2041 gtcagggagg gtaaaatgta gtgctgccaa ctgtctgctc ccacggtagg cagccagccc
     2101 agttcaacgg agaatacctg aattaaaagc atccccagcg caaatgccgg aaacgagatc
     2161 cccgacaccg caatggtcat actcaatcga tccggccaac gattacgcca gacggcggcg
     2221 ataattcccg ccgccatacc aaatatcacc gcccagacca tactggttat ggtcagccac
     2281 agcgttggca taaagcggct ggcaatctca tcggcaaccg gacgacgcga caccatcgac
     2341 aggccaaaat ccccctgcac agcattgctg atatagtgcc agaactggtg atacagcggc
     2401 tgatccaacc ccagctgctg acgcaccagt tctataacct gcgcatcagc ttcgagcccg
     2461 gcaatcaatc gcgccggatc gccgggcagc atatgcacaa ataaaaacac cagcaccgag
     2521 acgataaaca gcgtcggaat caaccccagt aagcgtttga taacgtaatt aagcattcca
     2581 ctccctgcgt tgcttattgc aaatccgcgt cttcaaagct gaagccggtg tctggcatga
     2641 tccaaaaacc ggtcaggttt ttactgtgtg ccgacaccag tttttctacc accagcggga
     2701 tccacggcga ctcttgccag atgatatcct gcgccgcctt atacaagcgg gtcttttccg
     2761 ccggatcatt agttttcagt gcctgagcca ggaagtcgtc cacctgttta ttgctgtaaa
     2821 atgcggtatt aaacagcgtc ggtggccagt tttgcgaggc gaacagtggc gatagcgccc
     2881 agtccgcttc gccggttgaa gccgaccagc cagtgtagaa catccgcacg ccgctctctt
     2941 tttgcccttt accttcaact tctgccgccc gctgtccggc atccatcgcg gtcacctggg
     3001 ctttaatccc gacctgcgct aactgctgct gggtaaattg cagcactttc tgcgcggtgc
     3061 tgtggttatg tgacgaccac agcgtggtac tgaaaccgtt gggatatccc gcctctttca
     3121 gtaattcgcg cgctttcact ggatcgtaag gccacggttt ataactttgc gcgtaggcga
     3181 tacttggcgg taccacacca gtagctggcg ttgcatagcc cgcaaaggca actttcacca
     3241 gcgccggacg gttaatggcg taattcagcg cctcacggac cttcgggtta tcgaacggct
     3301 tttgcgtcac gttcatactg atataacgct gcataattga cggactggcc atcaactcga
     3361 tatttttgtt tttctccagc agtgtggcct gctcgtaggg aatggggaaa gcaaactgcg
     3421 cttcaccggt ttgcagcatt gccgcgcggg tgttgttatc cgccaccgga cgccaggtta
     3481 tgctgtccag tttgggcaat cctggctgcc agtaacccgc gaattttttc accttcacaa
     3541 aatcggtctg attccaggta tccagttcat acggtccggt tcccaccgga tgaaaaccaa
     3601 tctccttgcc atatttttcc agcgctgccg gtgaaatcat cgcggtcgcc ggatgggcaa
     3661 gaatattaat aaacgctgag aacggctgtt tgagggtaat ctttaccgtt gtcggatcga
     3721 tcgcctccgt tttggcaata ttcttataca ggttatagcg tttaagatga ttcgccggat
     3781 cgctggcccg gtccagattc gcttttaccg ccactgcgtt gaaatcggtg ccatcctgga
     3841 atttaatgcc ttcccgcaat ttcacggtgt aagtaatgcc gtcatcggaa acggtataac
     3901 tctccgccag cacgtttttc agtttcatct ctttatccag accgaacagc ccctggtaaa
     3961 acgatttcgc tacggcctga gataacgtat cattcgcgtc atacggatcg agcgtggtga
     4021 aattcgatcc taccgccacc accacatctt tggcagcgaa tgcacaagat gccatcaacg
     4081 ctgtcgcaat gcccagcgcc actaacccac tacggtgtac agctcttgcc attttattct
     4141 ccgcctgaat gttatctacg catgaatgcg tattctgatt gtggttgtgc gacgtaatgc
     4201 cccggcccga cgcattgcaa cgagacggct gccacctctt cgccacgcag atgaatattg
     4261 ctgggaagat cgtccgacag cagtacacgc tgcggtcgtt gtcgggacgg ttcagcgacc
     4321 ggaactgccg ccagtaattt acgcgtataa ggatgctgcg ggttttcgaa gaccgcgcgc
     4381 cgtggaccaa tttcaacaat ttgcccgaga tacatcaccg ccacacgatg actaatccgc
     4441 tctaccacgg ccatatcgtg ggagataaac agatacgcaa tgccgaaatc acgctggaga
     4501 tcgagcaaca agttgataat ctgcccgcga atagaaacat ccagcgccga aacggcttcg
     4561 tcggcaatga tcacttttgg attcaatgcc aacgcgcgag caatgcagat gcgctggcgc
     4621 tgaccgccgg aaaactcatg cgggtaacgc caggcatgtt caggtaacag gcccacgcgc
     4681 tccagcaacc acgcaacgcg tgcagccgcg tctttacctg gcaataaacc gtgtacacgc
     4741 agcggttcga taatcgaatc accgatggtc tgacgtgggt ccagcgaagc gtaagggtcc
     4801 tgaaaaataa actgaatatc ccggcgcaat gcctgaagtt tgccgggtga caaggtatcg
     4861 attctctgac cgttaaagat aatttcgccg ccctgcgatt cgaccaggcg cagcaacgcc
     4921 cgcccggtag tggatttacc gctgccagac tcgcccacca gcgatagcgt ttcgccaggc
     4981 cagagatcaa aactgacttt ctcaacggca tgcacttccc gcgttacgcg attcaacaaa
     5041 ccgctgcgca aagggaaacg ggtgacaaga ttacgcactc gtaaaacagg ttcgccatcc
     5101 accaccgttt tctgctcgat ggggggggcc tgtttcgctg gatgttcaag cgatatcaac
     5161 gggaaacgtc ggggataatc caaccctttc atcgcaccaa gttgcggaac agcagctaac
     5221 agcgcacggg tgtaaggatg ttgcggtgca tgaaaaatct gttcgacggt acccgtttcc
     5281 accgcctcgc cctgatacat caccagtacc cgatcggcaa tctctgccac cacgcccata
     5341 tcgtgagtga taaagataac gcccatcgac atctcttttt gcaatacttt gattaattgc
     5401 aggatctgcg cctgaatagt gacatccagc gcggtggttg gctcatcggc aatcagcacc
     5461 gccgggcggc atgacagcgc catcgcaatc atcactcgct ggcgcatccc gccagagagt
     5521 tgatgcggat aacgtgaaag aatggtttgt gcctcaggaa tgcgtacctg atccagcatc
     5581 cgcttcgcct cgaccatcgc ttcttcacga ctggcgttct gatgcagacg aattgattcg
     5641 gcaatctgtt cacccacagt aaataccggg ttcagcgatg tcatcggctc ctgaaatatc
     5701 atcgccatat ccgcaccgcg aacatggcgc atttgtgcag cgctctgctc gctaagttca
     5761 atcacttcgc gactgcgccg ctgcaacagc attttatcgc actgtactaa accgcccgcc
     5821 tgttccaaca ggcgcatcaa tgccaacgca gtcactgact taccggagcc ggattcgcca
     5881 acaattgcca gcgtctcacc gcgttgcaga ctaaaagaga gattgcggac cgcagctatt
     5941 ttctgctggt cctgcataaa ggcaatattc agattttcaa ccgccagcac attaccggca
     6001 tcaagttcat cactgtgtgg caacggtgtc ccctttttca cggtagatac cggtggttgg
     6061 cgtatcgcct gcgtagcccc aggcgcgata cattccttcg gtgttaaacg gtagcgcgac
     6121 attcccttca tggtcgatag cgattaagcc accgctaccg ccaagcgcag ggagtttttc
     6181 cattactacc cgctcgcagg cttccgcgag acttaatccg ccgtaatcca ttaacgcggc
     6241 gatgtcatat gccgccagcg cgcggatgaa gacttcgccc gtgccgatac aagaaaccgc
     6301 cacactggcg ttattggcgt agcatccggc acccactaag ggactatcgc caactcgtcc
     6361 gggtaattta ttggtcattc cgcctgtgga cgtggctgcc gccaaattgc cgtctaaatc
     6421 caacgccacg gcccccacgg tgcccatttt ctgtttttca tccagtggcg caccgctatg
     6481 gtcgaggacg gttgcccctt ccttgcgcgc tgccagtagt tgttcataac gcaaaggcgt
     6541 ggagaaaatc tccggcgaga cgcgctccat gccacgagca aacgcaaaat tttctgcccc
     6601 ttcgccaatc atcatcacat gcgggctttg ctccatcacc agccgggcgg caagaaccgg
     6661 attacgcaga tgactaacgc ccgccaccgc accggctttc agggtgttac catccatcac
     6721 acaggcgtcc agttcatggg tttcatcacg cgtaaagaca gcgccaattc cggcgttaaa
     6781 cagtggacac tcttccagca gacgcaccgc ttccgtcacc acatccagcg cactttcgcc
     6841 cgcttccagc attttctgcc cggtttcaac aatggcagac aacgcctcaa tgtagcgtaa
     6901 ttcctgttgc agactcatct gcgcgcggct aattgcacct gcgccaccat gaattgcaat
     6961 gactgctttg cccatagtat tcgtccatta agggtggctc gccacttttg ctataaatat
     7021 cagaattttt atgaattact cattcaattt ttgaatatag aaagatggat atggcatgta
     7081 aaggcaggag ccaacgctac gcccgaggtg gcatacagtc tgtggcgttt ttctgacata
     7141 ataggcaaat tcgattttgc ctccgcagga gtgttttcat ggaatttacc accggattga
     7201 tgtcgctcga caccgcgctt aatgagatgc tttctcgcgt caccccactg accgcccagg
     7261 aaacgctgcc actggtacag tgttttggtc gtattctggc gagcgatgtc gtttcgccgc
     7321 ttgatgttcc ggggtttgat aactccgcaa tggacggcta cgcggtgcgt ttagccgata
     7381 ttgcctccgg gcaaccgctg cccgttgccg gtaaatcctt tgccggtcag ccgtatcatg
     7441 gtgaatggcc tgcgggcacc tgcattcgta ttatgaccgg tgcgccggtg ccggaaggct
     7501 gcgaagcggt ggtgatgcag gagcagactg aacaaacgga caatggcgtg cgttttactg
     7561 ctgaagtgcg tagcgggcaa aatattcgcc gtcgcggtga agatatctct gcaggtgcgg
     7621 ttgttttccc ggcgggaact cgcctgacta ccgcagagct gccagtaatt gcttcactgg
     7681 ggattgccga agttccggtg attcgtaaag tgcgtgtagc gcttttttct accggtgatg
     7741 aactccagtt gcccggtcag ccgctgggcg acggccaaat ctacgacacc aaccgtctcg
     7801 ccgtacacct gatgttagaa cagctgggtt gcgaggtaat caacttaggg attatccgcg
     7861 acgatcccca tgccctgcgc gccgcattta ttgaagccga cagccaggcg gatgtagtga
     7921 tcagttccgg cggtgtttca gtgggtgaag cggattacac caaaacgatt cttgaagagc
     7981 tgggggagat cgccttctgg aagctggcga ttaaaccagg taaaccgttc gcgttcggta
     8041 aactcagcaa tagctggttc tgcggcctgc cgggcaaccc ggtttcagcg acgctgacct
     8101 tctatcaact ggtacagcct ttgctggcaa aactaagcgg caacaccgcc agcggcctgc
     8161 ccgcgcgcca gcgcgtacgc acagcgtccc gcctgaaaaa aacgccagga cgtcttgatt
     8221 tccagcgcgg cgtgctgcaa cgcaacgccg acggtgaact tgaagtgacg accaccggac
     8281 atcagggttc acatatattt agctccttta gtctcggcaa ctgctttatc gtgctggaac
     8341 gcgatcgcgg caatgtggac gtgggcgaat gggtggaagt agaaccgttt aacgcgttgt
     8401 tcggaggcct gtaatggcgg aactcagcga tcaggagatg ctgcgctaca accggcaaat
     8461 cattctgcgc ggttttgatt ttgacggtca ggaggcgcta aaagattctc gtgtgctgat
     8521 cgtcggcctc ggtggactcg gctgcgcagc ttcgctgtat ctggcaagcg ccggtgtcgg
     8581 caacctgacg ctgctcgact tcgacacggt ttcgctctcg aatctgcaac gccagacact
     8641 gcacagtgat gccacggtcg ggcaaccgaa ggtggaatcc gcccgtgacg ccctgacgcg
     8701 gatcaaccca catatcgcga ttacgccagt caatgcactg ctagatgacg cagaacttgc
     8761 agcattgatt gctgaacacg atctggtgct cgactgtacg gataacgttg cggtacgtaa
     8821 tcaactgaac gcaggctgtt ttgccgcgaa ggtgccgctg gtttccggcg cggcaattcg
     8881 tatggaaggg caaatcacgg tcttcactta tcaggacggc gaaccgtgct atcgctgcct
     8941 tagccgtttg tttggcgaga acgcattaac ctgcgtagaa gcaggcgtga tggcaccgtt
     9001 gatcggcgta attggttcgt tgcaggcaat ggaagcgatc aaactgctgg caggttatgg
     9061 caaaccgacc agcgggaaaa tcgtcatgta cgatgcgatg acctgtcagt ttcgcgaaat
     9121 gaaactgatg cgcaatccgg ggtgtgaggt gtgcgggcag taattgccac ctccaggcct
     9181 gataagacgc gacaagcgtc gcatcaggca tttgctccag acgccagatg cggcgtgaac
     9241 gccttatctg gcctacggtt cgggcacaga tttccaagcg cggcaggcat cggtgcactc
     9301 cgccacgtca tcagcaattt atgaggatgc agaacgtccg gttaaatcga cgttctgcca
     9361 aacgctccct gccagtcctg ctcaaacttc gccacagcgg cttccaccgc cggatagcta
     9421 atcatctgtt gtgccacatc cagtggcaga gtaattgatt cacatcctgc cagtaagcag
     9481 tccagcgcct gacgcggggt tttgaaactc gctgccagca ctttcgcctg cggcgcatgc
     9541 attttcaata actggtgtaa gtcggtcaca gtctgaatgc cgctaccgcc ctgagcatca
     9601 atacgattaa cgtaaggcgc aacatattcc gcacctgcca gcgccgacag caacccttgt
     9661 gctgcgccat ataccgcggt tcccagcgtt ggaatccctt ccgcttttaa catcttaata
     9721 gctgccagcc cctcggcggt caccggaact ttcaccacga tatccgcaat aatagaacgc
     9781 aatttacgtg cgtcattcac catcccttca gcagtggtag ccattacctg ggcaaacaga
     9841 cgcccctgac cgcccatcgc ttcatgaagt tgcggaagca caacatccag cggtttttta
     9901 cccgcggcga taatgcttgg gttagtggtc acacccgcca gcggaaaaat acgtgacagc
     9961 gccttcaccg caacaacgtc tgaagtatcc agatacagtt ccataaccat cctcaaaatg
    10021 tactttttgt ctgaatgtag actatcacgg gaaaactctc tgaacagttg acgcacatca
    10081 atctaacttt cattcgaaag tattttaatg tttgaacgaa acagagaggc gactatgatt
    10141 ttcaatattc agcgctactc gacccatgat ggccccggta tccgcacggt cgtatttctt
    10201 aaaggctgtt cgctgggctg ccgctggtgt cagaacccgg aaagccgcgc ccgcacgcag
    10261 gatctgctgt atgacgcacg actgtgtctg gaaggctgcg agctgtgcgc taaggccgcg
    10321 ccggaagtga ttgagcgcgc gctgaatggt ttgcttattc atcgggaaaa gttaaccccg
    10381 gagcatctga cggcgttaac cgactgctgt ccgacacagg cattaaccgt gtgtggtgaa
    10441 gtgaaaagcg ttgaggagat catgacgacc gttctgcgcg ataaaccgtt ttacgatcgc
    10501 agcggcggcg gtttaacgct ttcgggtggt gagcccttta tgcagccgga aatggcgatg
    10561 gcgctactgc aagccagcca cgaggcaggc attcatactg cggtagaaac ctgtctgcat
    10621 gtgccgtgga aatatatcgc cccttctctg ccctatatcg atctgtttct tgccgattta
    10681 aaacacgttg ccgacgcgcc gtttaaacag tggaccgacg gtaacgccgc cagagtgctg
    10741 gataacctga aaaaactcgc cgcagcgggc aaaaaaatca ttatccgcgt gccgctgatt
    10801 cagggcttta atgccgacga aacctctgta aaagctatta ccgattttgc cgccgacgag
    10861 ctgcacgttg gcgaaattca tttcctgccc taccacacgc taggcatcaa caaatatcac
    10921 ttacttaatc tgccctatga cgccccggaa aaaccgcttg atgcgccaga actgctcgac
    10981 tttgcccagc agtatgcctg ccagaaaggg ttaaccgcga ccttacgagg ataacaacca
    11041 tgaccacact gaaactggac acgctcagcg accgcattaa agcgcacaaa aatgcgctgg
    11101 tgcatattgt gaaaccgcca gtctgtaccg agcgcgcgca gcactatacc gagatgtatc
    11161 aacaacatct cgataagccg atcccggtac gtcgcgcgct ggcactggcg catcacctgg
    11221 cgaatcgcac catctggatc aaacacgatg agttgatcat tggcaaccag gcaagcgaag
    11281 ttcgcgccgc gccgatcttc ccggaatata ctgtctcgtg gatcgaaaaa gagattgatg
    11341 atctggcaga tcgtcccggt gctggctttg cggtgagcga agagaacaaa cgcgttctgc
    11401 atgaagtgtg cccgtggtgg cgcggtcaga ccgtacagga tcgctgctac ggcatgttta
    11461 ccgatgagca aaaaggtctg ctggcgaccg gaatcattaa agcggaaggc aatatgacct
    11521 ccggcgatgc gcacctggcg gtgaatttcc cgctgctgct ggaaaaaggt cttgatggtc
    11581 tgcgcgagaa agtagcggaa cgtcgctcgc gcatcaatct gacggtactg gaagatttgc
    11641 acggcgaaca gttcctgaaa gcgattgata tcgtgctggt ggcagtcagc gaacacattg
    11701 aacgttttgc agccctggcg cgtgaaatgg ccgcgaccga aacccgcgaa agccgtcgcg
    11761 atgaactgct ggcgatagca gaaaactgcg atcttatcgc ccaccagccg ccgcagactt
    11821 tctggcaggc gctgcaactg tgttacttca tccaattgat attgcagatc gaatctaacg
    11881 gtcactcggt atcgtttggt cgtatggacc agtatctcta cccgtactat cgccgcgacg
    11941 ttgaactcaa ccagacactg gatcgcgaac acgccatcga attgctgcat agctgctggc
    12001 tgaaactgct ggaagtgaac aagatccgct ccggctcaca ctcaaaagcc tctgcgggaa
    12061 gtcctctgta tcagaacgtc accattggcg gacaaactct ggttgatggt caaccaatgg
    12121 acgcggtgaa tccactctct tacgctatcc tcgaatcctg cggtcgcctg cgttccactc
    12181 agcctaacct cagcgtgcgt taccatgcag gaatgagtaa tgatttcctc gacgcctgcg
    12241 tacaggtgat ccgttgcggc ttcgggatgc cggcgttcaa caacgacgaa atcgtgatcc
    12301 cggaatttat taaactcggt attgaaccgc aggacgctta cgactacgca gcgattggtt
    12361 gtatcgaaac cgccgtcggt ggcaaatggg gctatcgctg taccggcatg agctttatca
    12421 acttcgcccg cgtgatgctg gcggcgctgg aaggcgggcg tgatgccacc agcggcaaag
    12481 tgttcctgcc acaagaaaaa gcgttgtcgg caggcaactt taacaatttc gatgaagtaa
    12541 tggacgcgtg ggatacgcaa atccgttatt acacgcgtaa atcaatcgaa atcgaatatg
    12601 tcgtcgacac catgctggaa gagaacgtgc acgatattct ctgctcggcg ctggtggatg
    12661 actgtattga gcgagcgaaa agtatcaagc aaggcggcgc gaaatatgac tgggtttctg
    12721 gcctgcaggt tggcattgcc aacctcggca acagcctggc ggcagtgaag aaactggtat
    12781 ttgaacaggg cgcgattggt cagcaacagc tggctgccgc actggcggat gacttcgatg
    12841 gcctgactca tgagcaactg cgtcagcggc tgattaacgg tgcgccgaag tacggcaacg
    12901 acgatgatac cgtcgatacg ctgctggctc gcgcttatca gacctatatc gacgaactga
    12961 aacagtacca taacccgcgc tacggtcgtg gtccggttgg cggcaactat tacgcgggta
    13021 cctcgtctat ctccgctaac gtaccgtttg gcgcgcaaac tatggcaaca ccggacgggc
    13081 gtaaagccca caccccgctg gcagaaggcg caagcccggc ctccggtact gaccatcttg
    13141 gccctactgc ggtcattggc tcagtgggta aactgcctac ggcagcgatt ctcggcggcg
    13201 tgttgctcaa ccagaaactg aatccggcaa cgctggagaa cgaatctgac aagcagaaac
    13261 tgatgatcct gctgcgtacc ttctttgaag tgcataaagg ctggcatatt cagtacaaca
    13321 tcgtttcccg cgaaacgctg ctggaggcga aaaaacatcc ggatcagtac cgcgatctgg
    13381 tagtgcgtgt cgcgggctat tccgccttct tcaccgcgct ctctccagac gcacaggacg
    13441 atatcatcgc ccgtactgaa catatgctgt aatctcctcg gcccggcggc gtcgtcgccg
    13501 ggctaaatca ttcacatcat caatttcatc cttactttca ttcgaaatat aatttgtgct
    13561 ctgcgtcaca ttgttattac actgttacgg tttcattttc gagccaggag cacacctatg
    13621 agcgtaaaag ttatcgtcac agacatggac ggtacttttc ttaacgacgc caaaacgtac
    13681 aaccaaccac gttttatggc gcaatatcag gaactgaaaa agcgcggcat taagttcgtt
    13741 gtcgccagcg gtaatcagta ttaccagctt atttcgttct ttcctgagct aaaggatgag
    13801 atctcttttg tcgcggaaaa cggcgcactg gtttacgaac atggcaagca actgttccac
    13861 ggcgaactga cccgacatga atcgcggatt gttattggcg agttgctaaa agataagcaa
    13921 ctcaattttg tcgcctgcgg tctgcaaagt gcatatgtca gcgaaaatgc ccccgaagca
    13981 tttgtcgcac tgatggcaaa acactaccat cgcctgaaag ctgtaaaaga ttatcaggag
    14041 attgacgacg tactgttcaa gttttcgctc aacctgccag atgaacaaat cccgttagtg
    14101 atcgacaaac tgcacatagc gctcgatggc attatgaaac ccgtcaccag tggttttggc
    14161 tttatcgacc tgattattcc cggtctacat aaagcaaacg gtatttcgcg gttactgaaa
    14221 cgctgggatc tgtcaccgca aaatgtggta gcgattggcg acagcggtaa cgatgcggag
    14281 atgctgaaaa tggcgcgtta ttcctttgcg atgggcaatg ctgcggaaaa cattaaacaa
    14341 atcgcccgtt acgctaccga tgataataat catgaaggcg cgctgaatgt gattcaggcg
    14401 gtgctggata acacatcccc ttttaacagc tgacctctct accggagccg tgcgctccgg
    14461 ttttcccttc tttttacatc ctgtacatca catcaaatca ttaaacttgc attaacttat
    14521 ttataactta tattgtcgca tgcaaatcag aatggtttcg aatgaaagtt aatgagaggt
    14581 ggttatggct tctactttta ccagcgacac attgcctgcc gatcacaaag cagctatccg
    14641 tcagatgaag cacgcgctgc gggcgcagct tggcgacgtc cagcagatct ttaatcagct
    14701 aagcgatgac attgccacgc gagtggctga aatcaacgca ctcaaagcac agggcgatgc
    14761 cgtctggccg gtgctgtctt atgccgatat caaagcaggt catgttactg cagagcagcg
    14821 cgaacagatt aaacgtcgcg gttgtgcggt gataaaaggc catttccccc gcgaacaagc
    14881 gctaggctgg gatcagtcga tgctggacta tctggaccgc aaccgctttg acgaggtcta
    14941 caaaggcccc ggcgataatt tcttcgggac gctcagcgct tcacgtcccg agatttaccc
    15001 catctactgg tcgcaggcgc aaatgcaggc ccgccagagt gaagaaatgg cgaatgcgca
    15061 gtcgtttctc aatcgtctgt ggacatttga aagtgatgga aagcaatggt ttaacccgga
    15121 tgtgagcgtc atctaccctg accgtatccg ccgccgtccg cccggaacga cctccaaagg
    15181 tcttggagcg cataccgact ccggggcgct ggaacgctgg ctgcttccag cgtatcagcg
    15241 cgttttcgcc aacgtcttta atggcaatct ggcgcaatat gatccctggc atgcggcaca
    15301 tcgtacggaa gttgaagagt acacggtgga caacaccacc aaatgttccg tgtttcggac
    15361 attccaaggc tggacagcgc tctctaatat gctgcctggt caggggctgc tgcacgttgt
    15421 gcccattcct gaagccatgg cgtacgtact gttacgtccg ctgcttgatg atgtgccgga
    15481 ggatgaactg tgcggcgtag cgcccggaag agtgttgccg gtatcagagc aatggcatcc
    15541 actgttaatt gaggcgttaa ccagcattcc aaaactcgag gccggagact ccgtctggtg
    15601 gcactgcgac gtcatccatt ccgttgcccc cgttgaaaat caacagggtt ggggcaacgt
    15661 gatgtacatt cctgcggcac caatgtgcga gaaaaatctt gcctacgcgc acaaggtgaa
    15721 ggccgcactg gaaaaaggcg catcgccggg cgacttcccg cgcgaggact atgaaacaaa
    15781 ctgggaagga cgctttacgc ttgccgacct caacattcac ggtaagcgag cgttgggaat
    15841 cgacagctga gtgtcgtaca gcccttcccg ttccacggcg gtgttccctt ttcctgggcg
    15901 aacccgccgc actgactccc gcaccgccaa tcgtccgtgc agcagcagcg agcccaccgg
    15961 agcatccggg cgaatcagcc gctgttgcaa catttgcacc gcttcattgc ctagctcatc
    16021 gcgtggcaca tgcacagccg tcaggggaac atcctcgatt gccgcaaggt taaagccgtc
    16081 gatactcatc accgagacat cctgtggtac gcgcagacca cgacgatgta gcgcatttac
    16141 cgttcccgcc gccataaaat ccccgccgac cagaaacgcc gtcggcaacg tcttgccatt
    16201 ggcggcatcc agccacgctc cgaccttttc ttccgcttct ttagcgctaa aactgctgat
    16261 attcagcaaa tgacggtcat ggtgaaaggc cagattatgc tgacgccatg attctttaat
    16321 ccctgcaaac cgctgctcca tagtgtagcg gcgcaggcac atcacattca tcacctcccg
    16381 gtgacccatt tcaaacagat agcgggcggc gaacgcgcca attaaccgat gatccggcgc
    16441 aatcgccggg aggcgcattc gttcatcacg gcagttgatc agcacacaag gcttagcgag
    16501 atcggcggcc agatcatgga tgtgcggatc gtcaatcccc agcaggatcg ctgcctgcgt
    16561 atctgcttca ttcatccgcg agagaaagag cgcgggatcg ctgtcaaact cctccagcgc
    16621 gcagtaacgc agccgcactt catgcggcga taacgctttg ctgatgctct ggatcacacg
    16681 gtaataaaag atatcagatc gttggtcaaa agcgcgttgc ggcgcgaaaa taatcaggtt
    16741 gttcagcaac atacgacctg ctgccatgcc atccatcacg cccaactcac gcgcgcaggc
    16801 cagcacctgt tcgcgagcgc gtgcactggt attcgctttg ccagccagca cccgcgaaac
    16861 cgtactcggc gatagcccgg tacgtgcggc gatatcggcg attttcagct tcttgtccat
    16921 tttgtgatct gcctctaatt cggaaatgac agaattttca tgagcaaaaa agcccattct
    16981 gtctggcatt aaatccattt cagtaatgta tatcagtcac tcatggaaaa acgtgcacaa
    17041 aacccgcttt ctttcgcttt tagggtctct tactgtgaat tggtagaaca acttccatac
    17101 aaaaataatg gaaaaacgaa caataaagac tttatttatc aaaaagttaa aagtggtgta
    17161 acaactccat tctgacgttg ccgcacaaac cctataccat tctgtggagt gcatatgagt
    17221 cagggtatca ataacgatat gactgccagt aaaccgcgcc atatcgtcaa aaatctgcgt
    17281 tggtggatac tggtcctttt tttgctcggt gttactgtca actacatcac gcgtaactcg
    17341 ctgggtattc tcgcccccga actgaaaagc agcctaggga ttaccaccga acaatattcc
    17401 tggattgtag gtgctttcca gctcgcctat accctattcc aaccgttgtg cggatggctg
    17461 attgacgtca ttggcctcaa actgggcttt atgatctgcg cgggactgtg ggcactggcc
    17521 tgtctgatgc atgcgggggc gggaaactgg ggacatctgg cgctgttgcg cttctttatg
    17581 ggcggtgcgg aagccgcggc aaccccagca aacgccaaaa ccatcggtga atggtttcca
    17641 aaatcagaac gcccgatcgc cgcaggctgg gcaggcgtcg gtttttccat cggtgccatg
    17701 ctggccccac cgattatcta ttttgcccac gcttcattcg gctggcaggg tgcatttatg
    17761 ttcactggca tcctggcgat gctgtgggtg gtgctgtggt gggcgttcta tcacagcccg
    17821 gataagcatc ctaacctcag caaagccgag ctggacttta ttcgccagga taacgaagcg
    17881 cccccggtca agttaccctt tctgaccgca ctgaaaaccg tgggcaaaaa taaacgcttt
    17941 tacggtatcg ctatcccggc ctttatggca gaaccggcgt gggcggtgat gagtttctgg
    18001 gtgcctctat acctgtccaa aacctatggc atggacctga agcagatcgc attattcgcc
    18061 tggttgccgt tcctcgccgc tgatcttggc agtattgcca gcggctatct gaccaaactt
    18121 tacacccgcc tgtttgggct gagtcgtgtg aactccgttg tcgcatcatc ggtcagtggc
    18181 gcattcttga tggtttcgct ggcggtgatg gcctttacca ataacccgta tatcgccatc
    18241 gtccttatct ccatcggcgg cttcggtcac cagatcatct cctgcatgct gagcgcactg
    18301 gtggtggaat ccttcgacaa aggtcagatg gccactgtta acggcatgcg cggttctgcc
    18361 gcgtggatcg caagtttcct gttctccctg attatcgggg ttaccgctga caaaatcggc
    18421 tttaaccccc tgtttatcgc catgggcttc tttgacctga ttggcgcttt cttcctggtc
    18481 acctttattg ctgaacgtcg cgcaaaacgc gcctgagagt ggatgtaaac atgaaaacgc
    18541 tgaaaaactg gaccctgcac aaacagtctg cccaccacgt tgaactgctg gtcgatggcc
    18601 agcatatcct gtgcctgtac gtgctggaag aaaatctgtt ccgcgtgctg ttaaaacgca
    18661 aaggcgagct ggcgctagat cgcacctgga gcattgctcc ggcgcaggat gtgccatggg
    18721 aagggcgcag ccgcgaagat ctcagcggct ttagcctgcc cgcgtggaca gtgatccagc
    18781 acgacgacac cgtggttatc gccaccgagt cactgcgtgc aaccgttcac cagccgctgt
    18841 ggctggagtg gcactatcgt aacaacgaag gcgaatggct gccgttggtg aatgaccgcc
    18901 cgaccagcgc ttatctgctg aatgcccacg gcgatggcgt ggcacactat cagagccgtc
    18961 gtaaagatga acgcttttat ggcctcggag ataaagccgg cgatctgcaa cgcaacggta
    19021 aacgttacga gatgcgtaac ctcgacgcga tgggctacaa cgccgttagt actgacccgc
    19081 tgtacaaaca cattccattc accatcaccc gccgcgatga cattagcttc ggcctgtttt
    19141 atgacaacct cagcagctgc tggctggatc tgggcaacga gatcgacaac taccacaccg
    19201 cctatcgccg ctggcaggcg gaagcgggtg atatcgacta ctacctgttt accggcgaac
    19261 gcgtacttga tatcaccaaa gccttcgtac gcctgaccgg aaaaaccctg ttcggaccga
    19321 aatggagcct gggctacagc ggttcaacta tgcactacac cgatgcgccg gacgcacaaa
    19381 atcagttgat gaactttatc cgcctgtgtg aagaacatgc aattccctgc gattcgttcc
    19441 agctctcttc cggttatacc tcaattaacg gcaaacgcta tgtgttcaac tggaactacg
    19501 acaaagtgcc gcagccgaaa gtgatgagcc aggcatttca caatgctggt ttgcacctgg
    19561 ccgccaatat caagccgtgc ctgttgcagg atcacccacg ctataacgaa gtggcagaaa
    19621 gaggactgtt catccgtgat tcagaaacag atgaaccaga acgttccagc ttctgggatg
    19681 acgaaggttc taacctcgag tttactaatc cgcagaccat ccagtggtgg caggaaggcg
    19741 tgaccacaca gcttctggag atgggtatcg actccacctg gaacgacaac aacgaattcg
    19801 aagtatggga cggtgaagca cgctgtcacg gctttggtca gacgatcgcg atcaaacata
    19861 ttcgcccagt gatgccactg ctgatgatgc gagcctcgat ggaagcccag cagcgtttcg
    19921 ctccggaaaa acgtccgtac ttgatctccc gctccggctg cgcgggaatg cagcgctacg
    19981 tgcagacctg gagtggcgat aaccgcacta gctgggacac tctgcgctac aacacccgca
    20041 tgggtctggg gatgagcctc tccgggctgt ataacctcgg tcacgacgtc ggcggtttct
    20101 ccggtgataa gccggacccg gaactgtttg tacgctgggt acaaaacggc gtgatgcatc
    20161 cgcgctttac gattcactca tggaacgatg accataccgt aaacgaaccg tggatgtatc
    20221 cgggcgtcac tccggcgatc cgcagcgcta ttgagttgcg ctatcgcctg ctgccctact
    20281 tttataccct gatgtggcag gcatacgccg atgatgaacc aatgctacgt ccgactttcc
    20341 ttgaccacga gcacgacgtt cagacttttg aagagtgtga tgacttcctg cttggtcgcg
    20401 atattttggt tgccagcgtc gtagagcaag gcgagcgcca gcgccgtgta tggctgccag
    20461 ataacgaaac tggctggtac gatttctaca acggcgaatg gttcagtggc ggtcagtgga
    20521 tcaccctcga cgcaccgctg gagaaactgc cgctgatggt tcgtgccggg gctggtctac
    20581 cgctcagcaa acgcatcacc tatgtgagcg cagaacaaga cgatacccgc gaactgaaac
    20641 tgttcccgct gaaaggtgtg ggcacaacgt ccggcctgct gtttgaagac gatggcgaaa
    20701 gctggggcta ccaaactggt aatgcgcttt gggtggaatg ggaaatggtt tgcgatgggg
    20761 cgaccatcaa cctgaaagtc aacgcgcgcg gggattatcg tcctgcatgg agtgcactga
    20821 aagtgtcatt accattggaa gaaaaacgca cgctgctggt caatggtgtt gaagggagtg
    20881 aatggatgcg ttaaatcatt accggggccg ggaggcgact gcaccttacc cggtccagcc
    20941 atcgaatcct ttacctcagt atttcacgct gcaggaaggc agcaaacctg taaaccccgg
    21001 gagcttaaat aagtaagtga ccggggtgaa caggtgaagc caatgcatct gccgcatgaa
    21061 agacggcggg gaattaagcg tcgatccctt tactacgcag gtaatcttcg taattaccgc
    21121 taaagtcgat cgcgcgttcc ggggtgattt ccagaatgcg ggtcgccagg gagcttacga
    21181 actcacggtc gtgtgaaaca aagatcagcg tgccctgata cagttccagc gccatgttca
    21241 gtgactcgat ggattccata tccaggtggt tggtcggttc gtccatgatc agaatgttcg
    21301 gcttctgcat cattaactta ccaaacagca tccgcccttt ttccccaccg gaaagcactt
    21361 tagctggctt tttgatgtcg tcctggctga acagcaaacg accgagaatg ctgcgtaccg
    21421 cctgctcgtc atcgccttcc tgcttccact gactcatcca ttcgaacacg gtcagatcat
    21481 tttcaaactc atattcgtgg tcctgggcgt agtaaccaat gcgcgcgttt tcagaccatt
    21541 ttacggtgcc gctgtccggt tgcagatcgc ccaccagcgt tttcagcagc gttgatttac
    21601 cgacgccgtt ggtacccagt accgcaagtt tttcaccgac ctccagcagc aggttgagat
    21661 ttttaaacag tggaccgtta tcaaaccctt tggtcagacc ttccacttcc agcgcgttac
    21721 ggaacagttt cttatcctgt tcaaaacgga tgaacgggtt ctgacggctg gaggctttca
    21781 cctcttccag tttgatttta tcaatctggc gcgcgcgcga agttgcctgg cgagatttcg
    21841 aggcgttggc gctaaagcgg ctaacgaaag attgcaactc agcaatctgc gctttcttct
    21901 tggcgttatc ggccagcaga cgttcacgcg cctgggtcgc cgccgtcatg tactcatcgt
    21961 agttacccgg ataaacgcgc agctcgccgt aatccagatc cgccatgtgg gtacagacca
    22021 tgttaaggaa gtgacggtcg tgcgagatga tgatcatggt gctgtcacgc tcgttcagca
    22081 cctgttccag ccagcgaatg gtgtcgatgt cgaggttgtt ggtcggttcg tcgagcagga
    22141 gaatctccgg atcagcaaac agcgcctgcg ccagaagcac acgcagtttc cagccaggag
    22201 caacttcact catcgggccg tagtgctgtt ccactggaat tcccacgcca agcaacagtt
    22261 caccggcgcg agcttccgca gagtaaccgt ccatttcgcc gtatttaact tccagatcgg
    22321 ccactttata gccgtcttcc tcactcattt ccggcaaagc atagatgcgg tcacgctcct
    22381 gcttcacttc ccacaactct ttatgcccca tgatcaccgt atccagcaca gtgaactctt
    22441 caaaggcaaa ctgatcctga cgcagcttac caatgcgctc gttgggatcg agggaaacgt
    22501 tacccaaagt cggctctaag tcgccgccga ggatcttcat aaaggtggat ttaccactac
    22561 cgttcgcgcc aatcaggccg taacggttgc cgccgccgaa tttgacggaa atgttttcaa
    22621 acaacggctt actgccgaac tgcatggtga cgttactgga aactaacaca ggcgtatcct
    22681 gaaaagagat atgacaaacc gcgcattatg ccataagtct gaaagaagat cctgctcctc
    22741 gcttaaggtc tacactcaag ccaaaggcaa aaaatgtgat ttcgtacaca tctgatttca
    22801 ctgtgagctg gaatgaactt ataatgcgct tccaatactc taatattctc aacccaatgg
    22861 cctgccaggc acaaaatctc gcttaacatg aatatgaaat tgaaaacatt attcgcagcg
    22921 gccttcgctg ttgtcggctt ttgcagtacc gcctctgcgg taacttatcc tctgccaacc
    22981 gacgggagtc gcctggttgg tcagaatcag gtgatcacca ttcctgaagg taacactcag
    23041 ccgctggagt attttgccgc ggagtaccag atggggcttt ccaatatgat ggaagcgaac
    23101 ccgggtgtgg ataccttcct gccgaaaggc ggtactgtac tgaacattcc gcagcagctg
    23161 atcctgccgg ataccgttca tgaaggcatc gtcattaaca gtgcagagat gcgtctgtat
    23221 tactatccga aagggaccaa caccgttatc gtgctgccga tcggcattgg tcagttaggc
    23281 aaagatacgc ctatcaactg gaccaccaaa gttgagcgta agaaagcagg cccgacctgg
    23341 acgccgaccg ccaaaatgca cgcagagtac cgcgctgcgg gcgaaccgct tccggctgtc
    23401 gttccggcag gtccggataa cccgatgggg ctgtatgcac tctacatcgg tcgcctgtac
    23461 gctatccatg gcaccaacgc caacttcggt atcggcctgc gtgtaagtca tggttgtgtg
    23521 cgtctgcgta acgaagacat caaattcctg ttcgagaaag taccggtcgg tacccgcgta
    23581 cagtttattg atgagccggt aaaagcgacc accgagccag acggcagccg ttatattgaa
    23641 gtccataacc cgctgtctac caccgaagcc cagtttgaag gtcaggaaat tgtgccaatt
    23701 accctgacga agagcgtgca aacagtgacc ggtcagccag atgttgacca ggttgttctt
    23761 gatgaagcga ttaaaaaccg ctccgggatg ccggttcgtc tgaattaatc ttcaaaactt
    23821 aaagcaaaag gcggactcat aatccgcctt ttttatttgc cagaccctag ttggccggga
    23881 gtataactaa caaaacatat cccaccaacg ccgcccacaa cagcatcggt attgaataga
    23941 gatggaaacg ccaccagatg cggcgatcgt tcgccatacg tagcgcaatc aaatttgcca
    24001 gcgatccggg taataaccca aagccaccta cgtttaccgc ccataccagt aataaagacg
    24061 gcggcacata gttcagcaac aatatggtac tcggcacgtt actgatcacc tgcgataaac
    24121 cgattgccgt taaccataac ccgggttcag atagatgact cacgttaccc aacacgcctt
    24181 gcaacgctgg aagctgggtc agtaaatgga cgtcgataaa catcgccata aacaccagca
    24241 gcagcgtcca gtccacactg agcaccacgc gacgtgccag cagcgcaaag cctgccgcca
    24301 caatcaccag tccccacagc tcttgtttga actccagcgc cgtcagaaag acgatataca
    24361 gccccagaca actccacacc agccgcggtt tccactccgg tgtttgcacc cccgtatggt
    24421 attgcagcgc ctttccaggg aaacaacacc agcacaggag caggagcgtc agcatcattg
    24481 cgccagccag cggtgccatt tgggcaataa atccggcaaa cgaaagacca gaacgtcccc
    24541 agataagaat attttgcggg ttgccaattg gcgtcagtag cgaaccagcg ttgactgcca
    24601 gcgcctcaaa aataatcagc cgattaaccg ggatctcaca cagtcttttt agcgtgatag
    24661 tcagcggaac aacaataaac agcgcgacat cgttggtcag aaaggtagaa agcagcgccg
    24721 ccgccagcac cataaacatc gccagccgac gctccgtagc aaagcggcgc accattttgc
    24781 gccccagcac atcaaaataa ccgcttaact ccacaccttt ggtcagcagc atcaggccgc
    24841 ttaaggtgat gatggtgtgc cagtcgatag cagcaggcca ggatttcggt gcaaagggca
    24901 cgaaaaagct taatccgata ccaacaagaa ttaataactg aaaaaaacga tcgccttgca
    24961 gcgtgcgtaa aaaaggcagg ctcatttggc accgtgtttc tgggtaaaca aacgaaaagc
    25021 gtcgagcgtc tcttcactaa catggtgctc catgccttcc gcgtcgcgac gggcgatttc
    25081 cggactgacg cccaacacca acaagaaatt ttcgactatc tgatgtcgct cgcggctttc
    25141 ctgcgccagc ttctctcctt ctgccgttaa aaacacgcca cgccagggga tcatttcaat
    25201 cagccccatg gttgccagcc gcttaagcat tttagccacc gtcggttgcg aaactcccag
    25261 acgagcagcc atgtccacct gacgagcttc ccccacttcc ctgatcaagt cagaaatcag
    25321 ctcaacgtaa tcatcaataa gctcgcgccg atgcgcctct ctgacctggc ggaacccttc
    25381 aacgtgctct tccacgttca ctaactgcgt cacttttttt gctgttggcg tacctgcgcg
    25441 acgactcatt gtgcttcctc attatggtga cgcatacata gcgttctcat ttcgtgcgca
    25501 cattgtaaac cagagttgcg aaggtacaaa aaattaacgt tttagcaata gctacataat
    25561 atagcctgtg ctatatctgt atgtaatgca atcatccctc aaggatcgac gggattagca
    25621 agtcaggagg tcttatgaat gagttcaaga agtgtatgcg cgtgtttagt cattctccct
    25681 ttaaagtacg gttaatgctg ctctctatgt tgtgcgatat ggtcaacaac aaaccgcagc
    25741 aagataaacc ttccgataaa tagcggcgtc gcggtacgcc gcttcactcc tgcttttatg
    25801 caggcataac gcgttttggt ctgaaaaacc ccactttttg tcggatttgc aatccccttc
    25861 gcaaaagatt tgttcgtcag tagttgacct gaacggcggc tcgctctatc ttcttgcagc
    25921 cctacgtata ttgcggctcg cggatgcgga cccctttccc ctcttcacgc actcttgcag
    25981 gtattgaccc ttgacgccag ggtaagcaca tggcgtttgt tacgatagtg gcatatgaat
    26041 ttaaccctca aagaatcgct tgttacccgt agccgggtat ttagcccgtg gactgcgttc
    26101 tactttttac agtcgctatt aattaacctc ggcttaggtt accccttcag tttgctctac
    26161 accgctgcgt ttacggctat tttgcttttg ctatggcgaa cattgcctcg cgtacaaaaa
    26221 gttctggtcg gtgtcagttc gctggtggcg gcttgttatt tcccttttgc tcaggcctac
    26281 ggcgcgccta atttcaatac attgctggca ttgcactcca ccaatatgga agagtcgacc
    26341 gaaatcctga cgatttttcc gtggtacagc tacctggtcg gcttatttat ttttgcgctc
    26401 ggcgtaatag caatcaggcg aaaaaaagag aatgaaaaag cgcgctggaa taccttcgac
    26461 agcctgtgtc tggtattcag tgtggcgaca ttttttgttg ctcccgtgca aaacctggcc
    26521 tggggtggcg tatttaaact gaaagatact ggctatccgg tatttcgttt tgctaaggat
    26581 gtcatcgtca ataataacga ggtgattgaa gagcaagaac ggatggcaaa actttccgga
    26641 atgaaagata cctggacggt cactgccgtt aagccgaagt atcagaccta tgtggtggtg
    26701 atcggcgaaa gcgcgcgtcg cgatgccctc ggtgcctttg gcggtcactg ggacaatacc
    26761 ccgtttgcca gcagcgttaa cggtttgata tttgctgact acattgccgc cagtggctcc
    26821 acgcagaaat cacttggctt aacgctcaat cgcgtagtcg atggcaaacc acagtttcag
    26881 gataactttg tcaccctggc aaatcgcgcg ggcttccaga cctggtggtt ttccaaccag
    26941 ggccaaatcg gcgaatacga tactgctatc gccagcatcg ccaaacgtgc agatgaagtg
    27001 tacttcctga aagaaggtaa tttcgaagca gataaaaaca cgaaagacga agcgttactg
    27061 gatatgaccg ctcaagtgct ggcgcaagag cactcgcaac cgcagctgat tgttctgcat
    27121 ctgatgggct cgcatccgca ggcctgcgac aggacacaag gcaaatacga aacctttgtg
    27181 caatcgaaag aaacgtcgtg ctatctctat accatgacgc aaacggacga tttactgcgc
    27241 aagctgtacg atcagttacg caacagcggc agcagcttct ctctggttta cttttctgac
    27301 cacggtctgg cctttaaaga gcgcggcaaa gacgtgcaat accttgccca tgatgataaa
    27361 tatcagcaaa atttccaggt gccttttatg gtcatttcca gcgacgataa agcgcatcgg
    27421 gtgattaaag cccgccgctc agccaatgac ttcttaggct ttttctcgca gtggacggga
    27481 attaaagcga aggaaataaa tatcaaatac ccgtttatat ctgagaagaa agccgggccg
    27541 atatacatca ccaacttcca gttacagaag gtggattaca accatctcgg aaccgatatt
    27601 ttcgacccga aaccttaaaa caaaaaatcc gccccgagag gcggattttt tatatcacca
    27661 aagtgattag aagcggtaac caacaccggc aatccaggtg cctacgtcaa cgctacgaat
    27721 acggctctgc tcgtaagaga agtccagagc aacgttttcc atcgggttga actgcagacc
    27781 agcaccgtag gagaaaccgt agtcgctggt gtcgtgtttg taggtcgggt attcagtggt
    27841 ctggaattta ccataaccca cccccactac accgtagatg cttgcccagt cgttaatgcg
    27901 gtaagccgga ccagcagtga tgccgtagta ctggtttttg ttgtagtcac cagagcttgc
    27961 agtacggctt ttctcggtgt aagtgaaaga accgatcaca cccagcgggc tgttgtcttc
    28021 ttcatagcgg tatttcaggt tgaaaccgcc cattttgttc atttggccct gagcgtcgct
    28081 ctgtgcgtaa ccgccagtta cggtagaagt cgcagctacg gaagtacctg cggtgaaagc
    28141 cagaactgcg gccagtgctg aaagacatgc aatttttttc ataaccacct caaatgtgat
    28201 tcaaataagt cctaagtttt aaatatatca aaaattaatg ggaaactctt cgcgatttgt
    28261 gatgtctaac gggccatttc atgtaacaga acgtttccat acaccgctat ccatctaaat
    28321 ttaaatcact ttttcagaga actgcgtaag tattacgcat gttttccctg tcattcatcc
    28381 agattattcc taatcaccag actaatgatt ccatcaatcc tggcgcattt tagtcaaaac
    28441 gggggaaaat tttttcaaca aatgctcaac cagcattggg tatatccagt acactccacg
    28501 ctttacttaa gtctagatat ttgtgggaga aaggatgcct ggttcattac gtaaaatgcc
    28561 ggtctggtta ccaatagtca tattgctcgt tgccatggcg tctattcagg gtggagcctc
    28621 gttagctaag tcactttttc ctctggtggg cgcaccgggt gtcactgcgc tgcgtctggc
    28681 attaggaacg ctgatcctca tcgcgttctt taagccatgg cgactgcgct ttgccaaaga
    28741 gcaacggtta ccgctgttgt tttacggcgt ttcgctgggt gggatgaatt atctttttta
    28801 tctttctatt cagacagtac cgctgggtat tgcggtggcg ctggagttca ccggaccact
    28861 ggcggtggcg ctgttctctt ctcgtcgccc ggtagatttc gtctgggttg tgctggcggt
    28921 tcttggtctg tggttcctgc taccgctggg gcaagacgtt tcccatgtcg atttaaccgg
    28981 ctgtgcgctg gcactggggg ccggggcttg ttgggctatt tacattttaa gtgggcaacg
    29041 cgcaggagcg gaacatggcc ctgcgacggt ggcaattggt tcgttgattg cagcgttaat
    29101 tttcgtgcca attggagcgc ttcaggctgg tgaagcactc tggcactggt cggttattcc
    29161 attgggtctg gctgtcgcta ttctctcgac cgctctgcct tattcgctgg aaatgattgc
    29221 cctcacccgt ttgccaacac ggacatttgg tacgctgatg agcatggaac cggcgctggc
    29281 tgccgtttcc gggatgattt tcctcggaga aacactgaca cccatacagc tgctggcgct
    29341 cggcgctatc atcgccgctt caatggggtc tacgctgaca gtacgcaaag agagcaaaat
    29401 aaaagaatta gacattaatt aaatttacat ttctgcatgg ttatgcataa ccatgcagaa
    29461 tttctcgcta cttttcctct acaccgtctt tatatatcga attatgcaaa agcatattta
    29521 ttccgaaaat tcctggcgag cagataaata agaattgttc ttatcaatat atctaactca
    29581 ttgaatcttt attagttttg tttttcacgc ttgttaccac tattagtgtg ataggaacag
    29641 ccagaatagc ggaacacata gccggtgcta tacttagtct cgttaattac tgggacataa
    29701 catcaagagg atatgaaatt atgagtaccg ctaaattagt taaatcaaaa gcgaccaatc
    29761 tgctttatac ccgcaacgat gtctccgaca gcgagaaaaa agcaacagta gagttgctga
    29821 atcgccaggt tatccagttt attgatcttt ctttgattac caaacaagcg cactggaaca
    29881 tgcgcggcgc taacttcatt gccgtacatg aaatgctgga tggcttccgc accgcactga
    29941 tcgatcatct ggataccatg gcagaacgtg cagtgcagct gggcggtgta gctctgggga
    30001 ccactcaagt tatcaacagc aaaaccccgc tgaaaagtta cccgctggac atccacaacg
    30061 ttcaggatca cctgaaagaa ctggctgacc gttacgcaat cgtcgctaat gacgtacgca
    30121 aagcgattgg cgaagcgaaa gatgacgaca ccgcagatat cctgaccgcc gcgtctcgcg
    30181 acctggataa attcctgtgg tttatcgagt ctaacatcga ataaatccat cgctgatggt
    30241 gcagaacttt agtacccgat aaaagcggct tcctgacagg aggccgtttt gttttgcagc
    30301 ccacctcaac gcacttattt agtgcatcca tctgctatct ccagctgatt aagtaaattt
    30361 tttgtatcca catcatcaca caatcgttac ataaagattg ttttttcatc aggttttacg
    30421 ctaaataatc actgtgttga gtgcacaatt ttagcgcacc agattggtgc cccagaatgg
    30481 tgcatcttca gggtattgcc ctataaatcg tgcatcacgt ttttgccgca tctcgaaaaa
    30541 tcaaggagtt gcaaaactgg cacgattttt tcatatatgt gaatgtcacg caggggatcg
    30601 tcccgtggat agaaaaaagg aaatgctatg aagtctgtat taaaagtttc actggctgca
    30661 ctgaccctgg cttttgcggt ttcttctcat gccgcggata aaaaattagt tgtcgcgacg
    30721 gataccgcct tcgttccgtt tgaatttaaa cagggcgata aatatgtggg ctttgacgtt
    30781 gatctgtggg ctgccatcgc taaagagctg aagctggatt acgaactgaa gccgatggat
    30841 ttcagtggga tcattccggc actgcaaacc aaaaacgtcg atctggcgct ggcgggcatt
    30901 accatcaccg acgagcgtaa aaaagcgatc gatttctctg acggctacta caaaagcggc
    30961 ctg
//
LOCUS       DAJTZL010000032        29940 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.032, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000032 DAJTZL010000000
VERSION     DAJTZL010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29940
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.032"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            248..631
                     /gene="secE"
                     /locus_tag="OO544_001275"
     CDS             248..631
                     /gene="secE"
                     /locus_tag="OO544_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HCR6766912.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            633..1178
                     /gene="nusG"
                     /locus_tag="OO544_001276"
     CDS             633..1178
                     /gene="nusG"
                     /locus_tag="OO544_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HCR6766913.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1337..1765
                     /gene="rplK"
                     /locus_tag="OO544_001277"
     CDS             1337..1765
                     /gene="rplK"
                     /locus_tag="OO544_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HCR6766914.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1769..2473
                     /gene="rplA"
                     /locus_tag="OO544_001278"
     CDS             1769..2473
                     /gene="rplA"
                     /locus_tag="OO544_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HCR6766915.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            2886..3383
                     /gene="rplJ"
                     /locus_tag="OO544_001279"
     CDS             2886..3383
                     /gene="rplJ"
                     /locus_tag="OO544_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HCR6766916.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3450..3815
                     /gene="rplL"
                     /locus_tag="OO544_001280"
     CDS             3450..3815
                     /gene="rplL"
                     /locus_tag="OO544_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HCR6766917.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKSLEEAGAEVEVK"
     gene            4135..8163
                     /gene="rpoB"
                     /locus_tag="OO544_001281"
     CDS             4135..8163
                     /gene="rpoB"
                     /locus_tag="OO544_001281"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HCR6766918.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8240..12463
                     /gene="rpoC"
                     /locus_tag="OO544_001282"
     CDS             8240..12463
                     /gene="rpoC"
                     /locus_tag="OO544_001282"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HCR6766919.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(12705..13838)
                     /gene="thiH"
                     /locus_tag="OO544_001283"
     CDS             complement(12705..13838)
                     /gene="thiH"
                     /locus_tag="OO544_001283"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HCR6766920.1"
                     /translation="MKTFSDRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEPLAQRAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYTRHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(13835..14605)
                     /gene="thiG"
                     /locus_tag="OO544_001284"
     CDS             complement(13835..14605)
                     /gene="thiG"
                     /locus_tag="OO544_001284"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HCR6766921.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAEMLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSHFAHATSPLTGFLEASA"
     gene            complement(14607..14807)
                     /gene="thiS"
                     /locus_tag="OO544_001285"
     CDS             complement(14607..14807)
                     /gene="thiS"
                     /locus_tag="OO544_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HCR6766922.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(14791..15546)
                     /gene="thiF"
                     /locus_tag="OO544_001286"
     CDS             complement(14791..15546)
                     /gene="thiF"
                     /locus_tag="OO544_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HCR6766923.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDIDRPKSQVSHQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVARADVVLDCTDNMATRQEINAACVVLNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQEPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(15539..16174)
                     /gene="thiE"
                     /locus_tag="OO544_001287"
     CDS             complement(15539..16174)
                     /gene="thiE"
                     /locus_tag="OO544_001287"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HCR6766924.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     NEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(16174..18069)
                     /gene="thiC"
                     /locus_tag="OO544_001288"
     CDS             complement(16174..18069)
                     /gene="thiC"
                     /locus_tag="OO544_001288"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HCR6766925.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTRTRLADDGLDELRFSGLLTPKRAKTGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAVHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(18150..18250)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18303..18779)
                     /gene="rsd"
                     /locus_tag="OO544_001289"
     CDS             complement(18303..18779)
                     /gene="rsd"
                     /locus_tag="OO544_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HCR6766926.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            18874..19647
                     /gene="nudC"
                     /locus_tag="OO544_001290"
     CDS             18874..19647
                     /gene="nudC"
                     /locus_tag="OO544_001290"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HCR6766927.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLVQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMTEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            19687..20751
                     /gene="hemE"
                     /locus_tag="OO544_001291"
     CDS             19687..20751
                     /gene="hemE"
                     /locus_tag="OO544_001291"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HCR6766928.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            20761..21432
                     /gene="nfi"
                     /locus_tag="OO544_001292"
     CDS             20761..21432
                     /gene="nfi"
                     /locus_tag="OO544_001292"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HCR6766929.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGVDVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLMVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            21475..22065
                     /locus_tag="OO544_001293"
     CDS             21475..22065
                     /locus_tag="OO544_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HCR6766930.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGESNIGIIFQQ"
     gene            22252..22524
                     /gene="hupA"
                     /locus_tag="OO544_001294"
     CDS             22252..22524
                     /gene="hupA"
                     /locus_tag="OO544_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-alpha"
                     /protein_id="HCR6766931.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            22537..23232
                     /locus_tag="OO544_001295"
     CDS             22537..23232
                     /locus_tag="OO544_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HCR6766932.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALLLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(23234..23659)
                     /gene="zraP"
                     /locus_tag="OO544_001296"
     CDS             complement(23234..23659)
                     /gene="zraP"
                     /locus_tag="OO544_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HCR6766933.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            23897..25273
                     /gene="zraS"
                     /locus_tag="OO544_001297"
     CDS             23897..25273
                     /gene="zraS"
                     /locus_tag="OO544_001297"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HCR6766934.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLDAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGATFTLWLPVT
                     ITRKGPQG"
     gene            25270..26595
                     /gene="zraR"
                     /locus_tag="OO544_001298"
     CDS             25270..26595
                     /gene="zraR"
                     /locus_tag="OO544_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HCR6766935.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVSASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADRRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAVHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIAGTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(26592..27881)
                     /gene="purD"
                     /locus_tag="OO544_001299"
     CDS             complement(26592..27881)
                     /gene="purD"
                     /locus_tag="OO544_001299"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HCR6766936.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGMKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVAGGKVFHAGTKLADNEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(27893..29482)
                     /gene="purH"
                     /locus_tag="OO544_001300"
     CDS             complement(27893..29482)
                     /gene="purH"
                     /locus_tag="OO544_001300"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HCR6766937.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7015 a         7804 c         8245 g         6876 t
ORIGIN      
        1 gcaaaagttc tgagctaatt gccgataaca tttgacgcaa tgcgcactaa aagggcatca
       61 tttgatgccc tttttgcacg ctttcgtacc agaacctggc tcatcagtga ttttctttgt
      121 cataatcatt gctgagacag gctctgttga gggcgtataa tccgaaaagc taatacgcgt
      181 ttcgatttgg tttgcctcgc gatcgcgggg tgaaaatgtt tgtagaaaac ttctgacagg
      241 ttggtttatg agtgcgaata ccgaagctca aggaagcggg cgcggcctgg aagcgatgaa
      301 gtgggtcgtt gtggtggcat tgctcctggt ggcgattgtc ggcaactacc tttatcgcga
      361 cattatgctg ccgctgcgtg cgctggccgt agtaattctg attgctgcag cgggtggtgt
      421 cgcgctgtta acgacaaaag gtaaagctac cgttgctttt gcccgtgaag cgcgtaccga
      481 agtccgtaag gtcatttggc cgactcgcca ggaaacattg cacaccacgc tgattgtggc
      541 tgcggttacc gcagtaatgt cactgatcct gtggggactg gatggtattc tggttcgcct
      601 ggtatccttt atcactggcc tgaggttctg agatgtctga agctcctaaa aagcgctggt
      661 acgtcgttca ggcgttttcc ggttttgaag gccgcgtagc aacgtcgcta cgtgagcata
      721 tcaaattaca caacatggaa gatttgtttg gtgaagtcat ggtaccaacc gaagaagtgg
      781 ttgaaatccg tggcggtcag cgtcgcaaaa gcgaacgtaa attcttccct ggctacgtcc
      841 tcgttcagat ggtgatgaac gacgcgagct ggcacctggt gcgcagcgta ccgcgtgtga
      901 tgggcttcat cggcggtact tccgatcgtc ctgcgccaat cagcgataaa gaagtcgatg
      961 cgattatgaa ccgcctgcag caggttggtg ataagccgcg tccgaaaacg ctgtttgaac
     1021 cgggtgaaat ggtccgtgtt aatgatggtc cgttcgctga cttcaacggt gttgttgaag
     1081 aagtggatta cgagaaatct cgtctgaaag tgtctgtttc tatcttcggt cgtgcgaccc
     1141 cggtagagct ggacttcagc caggttgaaa aagcctaacc cagcgatcaa aaaagcggcg
     1201 atttaatcgt tgcacaaggc gtgagattgg aatacaattt cgcgcctttt gtttttatgg
     1261 gccttgcccg taaaacgatt ttttatatca cggggagcct ctcagaggcg ttattaccca
     1321 acttgaggaa tttataatgg ctaagaaagt acaagcctat gtcaagctgc aggttgcagc
     1381 tggtatggct aacccgagtc cgccagtagg tccggctctg ggtcagcagg gcgtaaacat
     1441 catggaattc tgcaaagcgt tcaacgcaaa aactgattcc atcgaaaaag gtctgccgat
     1501 tccggtagta atcaccgttt acgctgaccg ttctttcact ttcgttacca agactccgcc
     1561 ggcagcagtt ctgctgaaaa aagcggctgg tatcaagtct ggttccggta agccgaacaa
     1621 agacaaagtg ggtaaaattt cccgcgctca gctgcaggaa atcgcgcaga ccaaagctgc
     1681 cgacatgact ggtgccgaca ttgaagcgat gactcgctcc atcgaaggta ctgcacgttc
     1741 catgggcctg gtagtggagg actaagaaat ggctaaactg accaagcgca tgcgtgttat
     1801 ccgcgagaaa gttgatgcaa ccaaacagta cgacatcaac gaagctatcg cactgctgaa
     1861 agagctggcg actgctaaat tcgtagaaag cgtggacgta gctgttaacc tcggcatcga
     1921 cgctcgtaaa tctgaccaga acgtacgtgg tgcaactgta ctgccgcacg gtactggccg
     1981 ttccgttcgc gtagccgtat ttacccaagg tgcaaacgct gaagctgcta aagctgcagg
     2041 cgcagaactg gtaggtatgg aagatctggc tgaccagatc aagaaaggcg aaatgaactt
     2101 tgacgttgtt attgcttctc cggatgcaat gcgcgttgtt ggccagctgg gccaggttct
     2161 gggtccgcgc ggcctgatgc caaacccgaa agtgggtact gtaacgccga acgttgctga
     2221 agcggttaaa aacgctaaag ctggccaggt tcgttaccgt aacgacaaaa acggcatcat
     2281 ccacaccacc atcggtaaag tggactttga cgctgacaaa ctgaaagaaa acctggaagc
     2341 tctgctggtt gcgctgaaaa aagcaaaacc gactcaggcg aaaggcgtgt acatcaagaa
     2401 agttagcatc tccaccacca tgggtgcagg tgttgcagtt gaccaggctg gcctgagcgc
     2461 ttctgtaaac taatgccttt acgtgggcgg tgattttgtc tacaatctta cccccacata
     2521 taatgcttaa tgcagacgta tatccgagat attcgggttg tggcaaggcg gcaactgagt
     2581 gagtcgccag gagcatagct aactatgtga ctggtgcgaa tgaaggaagc caacgccgcc
     2641 acaagttgaa tagcgacgga tagaaaagat ttgttcgttg gagcctggcc tatccaggcc
     2701 tccgtcgaag accgcaggag tttcgcaaga aacttaatcc cctgcgtaga cggtgacaga
     2761 acgctaagat tattctttta tattctggct tgtttctgct caccgtaatt aagacgctct
     2821 ctccgcttgg aggagtgaag tgagttccag agattttctc tggcaaacat ccaggagcaa
     2881 agctaatggc tttaaatctt caagacaaac aagcgattgt tgctgaagtc agcgaagtag
     2941 ccaaaggcgc gctgtctgca gtagttgcgg attcccgtgg cgtaactgta gataaaatga
     3001 ctgaactgcg taaagcaggt cgcgaagctg gcgtatacat gcgtgttgtt cgtaacaccc
     3061 tgctgcgccg tgctgttgaa ggtactccgt tcgagtgcct gaaagacgcg tttgttggtc
     3121 cgaccctgat tgcatactct atggaacacc cgggcgctgc tgctcgtctg ttcaaagagt
     3181 tcgcgaaagc gaatgcaaaa tttgaggtca aagccgctgc ctttgaaggt gagctgatcc
     3241 cggcgtctca gatcgaccgc ctggcaactc tgccgaccta cgaagaagca attgcacgcc
     3301 tgatggcaac catgaaagaa gcttcggctg gtaaactggt tcgtactctg gctgctgtac
     3361 gcgatgcgaa agaagctgct taatcgcagt tatcttttta acgcattcgc ttacgtataa
     3421 acttattctg atattcagga acaatttaaa tgtctatcac taaagatcaa atcattgaag
     3481 cagttgcagc tatgtctgta atggacgttg tagaactgat ctctgcaatg gaagaaaaat
     3541 tcggtgtttc cgctgctgct gctgtagctg tagctgctgg cccggttgaa gctgctgaag
     3601 aaaaaactga attcgacgta attctgaaag ctgctggcgc taacaaagtt gctgttatca
     3661 aagcagtacg tggcgcaact ggcctgggtc tgaaagaagc taaagacctg gtagaatctg
     3721 caccggctgc tctgaaagaa ggcgtgagca aagacgacgc agaagcactg aaaaaatccc
     3781 tggaagaagc tggcgctgaa gttgaagtta aataagccaa cccttccggt tgcagcctga
     3841 gaaatcaggc tgatggctgg tgactttttg gtcaccagcc tttttgcgct gtaaggcgcc
     3901 agtagcgttt cacactgttt gactactgct gtgcctttca atgcttgttt ctatcgacga
     3961 cttaatatac tgcgacagga cgtccgttct gtgtaaatcg caatgaaatg gtttaagcgt
     4021 gatagcaaca ggcattgcgg aaagtgttcc attttccggt caacaaaata gtgttgcaca
     4081 aactgtccgc tcaatggaca gatgggtcga cttgtcagcg agctgaggaa ccctatggtt
     4141 tactcctata ccgagaaaaa acgtattcgt aaggattttg gtaaacgtcc acaagttctg
     4201 gatgtacctt atctcctttc tatccagctt gactcgtttc agaaatttat cgagcaagat
     4261 cctgaagggc agtatggtct ggaagctgct ttccgttccg tattcccgat tcagagctac
     4321 agcggtaatt ccgagctgca atacgtcagc taccgccttg gcgaaccggt gtttgacgtc
     4381 caggaatgtc aaatccgtgg cgtgacctat tccgcaccgc tgcgcgttaa actgcgtctg
     4441 gtgatctatg agcgcgaagc gccggaaggc accgtaaaag acattaaaga acaagaagtc
     4501 tacatgggcg aaattccgct catgacagac aacggtacct ttgttatcaa cggtactgag
     4561 cgtgttatcg tttcccagct gcaccgtagt ccgggcgtct tctttgactc cgacaaaggt
     4621 aaaacccact cttcgggtaa agtgctgtat aacgcgcgca tcatccctta ccgtggttcc
     4681 tggctggact tcgaattcga tccgaaggac aacctgttcg tacgtatcga ccgtcgccgt
     4741 aaactgcctg cgaccatcat tctgcgcgcc ctgaactaca ccacagagca gatcctcgac
     4801 ctgttctttg aaaaagttat ctttgaaatc cgtgataaca agctgcagat ggagctggtg
     4861 ccggaacgcc tgcgtggtga aactgcatct ttcgacatcg aagctaacgg taaagtgtac
     4921 gtagaaaaag gccgccgtat cactgcgcgc cacattcgcc agctggaaaa agacgacgtc
     4981 aaactgatcg aagtcccggt tgagtacatc gcaggtaaag tggttgctaa agactatatt
     5041 gatgagtcta ccggcgagct gatctgcgca gcgaacatgg agctgagcct ggatctgttg
     5101 gctaagctga gccagtctgg tcacaagcgt attgaaacgc tgttcaccaa cgacctggat
     5161 cacggcccgt atatctctga aaccttacgt gtcgacccaa ctaacgaccg tctgagcgca
     5221 ctggtagaaa tctaccgcat gatgcgccct ggcgagccgc cgactcgtga agcagctgaa
     5281 agcctgttcg agaacctgtt cttctccgaa gaccgttatg acctgtctgc ggttggtcgt
     5341 atgaagttca accgttctct gctgcgcgaa gaaatcgaag gttccggtat cctgagcaaa
     5401 gacgacatca ttgatgttat gaaaaagctc atcgatatcc gtaacggtaa aggcgaagtc
     5461 gatgatatcg accacctcgg caaccgtcgt atccgttctg ttggcgaaat ggcggaaaac
     5521 cagttccgcg ttggcctggt acgtgtagag cgtgcggtga aagagcgtct gtctctgggc
     5581 gatctggata ccctgatgcc tcaggacatg atcaacgcca agccgatttc cgcagcagtg
     5641 aaagagttct tcggttccag ccagctgtct cagtttatgg accagaacaa cccgctgtct
     5701 gagattacgc acaaacgtcg tatctccgca ctcggcccgg gtggtctgac ccgtgaacgt
     5761 gcaggcttcg aagttcgaga cgtacacccg actcattacg gtcgcgtatg tccaatcgaa
     5821 acccctgaag gtccgaacat cggtctgatc aactctttgt ccgtgtacgc acagactaac
     5881 gaatacggct tccttgagac tccgtatcgt aaggtgaccg acggtgttgt aactgacgaa
     5941 attcactacc tttctgctat cgaagaaggc aactacgtta tcgcccaggc gaactccaac
     6001 ctggatgaag aaggccactt cgtagaagac ctggtaactt gccgtagcaa aggcgaatcc
     6061 agcttgttca gccgtgacca ggttgactac atggacgtat ccacccagca ggtggtatcc
     6121 gtcggtgcgt ccctgatccc gttcctggaa cacgatgacg ccaaccgtgc attgatgggt
     6181 gcgaacatgc aacgtcaggc cgttccgact ctgcgcgctg ataagccgct ggttggtact
     6241 ggtatggaac gtgctgttgc cgttgactcc ggtgtaactg cggtagctaa acgtggtggt
     6301 gtcgttcagt acgtggatgc ttcccgtatc gttatcaaag ttaacgaaga cgagatgtat
     6361 ccgggtgaag caggtatcga catctacaac ctgaccaaat acacccgttc taaccagaac
     6421 acctgtatca accagatgcc gtgtgtgtct ctgggtgaac cggttgaacg tggcgacgtg
     6481 ctggcagacg gtccgtccac cgacctcggt gaactggcgc ttggtcagaa catgcgcgta
     6541 gcgttcatgc cgtggaatgg ttacaacttc gaagactcca tcctcgtatc cgagcgtgtt
     6601 gttcaggaag accgtttcac caccatccac attcaggaac tggcgtgtgt gtcccgtgac
     6661 accaagctgg ggccggaaga gatcaccgct gacatcccga acgtgggtga agctgcgctc
     6721 tccaaactgg atgaatccgg tatcgtttac attggtgcgg aagtgaccgg tggcgacatt
     6781 ctggttggta aggtaacgcc gaaaggtgaa actcagctga ccccagaaga aaaactgctg
     6841 cgtgcgatct tcggtgagaa agcctctgac gttaaagact cttctctgcg cgtaccaaac
     6901 ggtgtatccg gtacggttat cgacgttcag gtctttactc gcgatggcgt agaaaaagac
     6961 aaacgtgcgc tggaaatcga agaaatgcag ctcaaacagg cgaagaaaga cctgtctgaa
     7021 gaactgcaga tcctcgaagc gggtttgttc agccgtatcc gtgctgtgct ggtagccggt
     7081 ggcgttgaag ctgagaagct cgacaaactg ccgcgcgatc gctggctgga gctgggcctg
     7141 accgacgaag agaaacaaaa tcagctggaa cagctggctg agcagtatga cgaactgaaa
     7201 cacgagttcg agaagaaact cgaagcgaaa cgccgcaaaa tcacccaggg cgacgatctg
     7261 gcaccgggcg tgctgaagat tgttaaggtg tatctggcgg ttaaacgccg tatccagcct
     7321 ggtgacaaga tggcaggtcg tcacggtaac aagggtgtaa tttctaagat caacccgatc
     7381 gaagatatgc cttacgatga aaacggtacg ccggtagaca tcgtactgaa cccgctgggc
     7441 gtaccgtctc gtatgaacat cggtcagatc ctcgaaaccc acctgggtat ggctgcgaaa
     7501 ggtatcggcg acaagatcaa cgccatgctg aaacagcagc aagaagtcgc gaaactgcgc
     7561 gaattcatcc agcgtgcgta cgatctgggc gctgacgttc gtcagaaagt tgacctgagt
     7621 accttcagcg atgaagaagt tatgcgtctg gctgaaaacc tgcgcaaagg tatgccaatc
     7681 gcaacgccgg tgttcgacgg tgcgaaagaa gcagaaatta aagagctgct gaaacttggc
     7741 gacctgccga cttccggtca gatccgcctg tacgatggtc gcactggtga acagttcgag
     7801 cgtccggtaa ccgttggtta catgtacatg ctgaaactga accacctggt cgacgacaag
     7861 atgcacgcgc gttccaccgg ttcttacagc ctggttactc agcagccgct gggtggtaag
     7921 gcacagttcg gtggtcagcg tttcggggag atggaagtgt gggcgctgga agcatacggc
     7981 gcagcataca ccctgcagga aatgctcacc gttaagtctg atgacgtgaa cggtcgtacc
     8041 aagatgtata aaaacatcgt ggacggcaac catcagatgg agccgggcat gccagaatcc
     8101 ttcaacgtat tgttgaaaga gattcgttcg ctgggtatca acatcgaact ggaagacgag
     8161 taattctcgc tcaaacaggt cactgctgtc ggggtaaaac ccggcagcgg attgtgctaa
     8221 ctccgacggg agcaaatccg tgaaagattt attaaagttt ctgaaagcgc agactaaaac
     8281 cgaagagttt gatgcgatca aaattgctct ggcttcgcca gacatgatcc gttcatggtc
     8341 tttcggtgaa gttaaaaagc cggaaaccat caactaccgt acgttcaaac cagaacgtga
     8401 cggccttttc tgcgcccgta tctttgggcc ggtaaaagat tacgagtgcc tgtgcggtaa
     8461 gtacaagcgc ctgaaacacc gtggcgtcat ctgtgagaag tgcggcgttg aagtgaccca
     8521 gactaaagta cgccgtgagc gtatgggcca catcgaactg gcttccccga ctgcgcacat
     8581 ctggttcctg aaatcgctgc cgtcccgtat cggtctgctg ctcgatatgc cgctgcgcga
     8641 tatcgaacgc gtactgtact ttgaatccta tgtggttatc gaaggcggta tgaccaacct
     8701 ggaacgtcag cagatcctga ctgaagagca gtatctggac gcgctggaag agttcggtga
     8761 cgaattcgac gcgaagatgg gtgcggaagc aatccaggct ctgctgaaga gcatggatct
     8821 ggagcaagag tgcgaacagc tgcgtgaaga gctgaacgaa accaactccg aaaccaagcg
     8881 taaaaagctg accaagcgta tcaaactgct ggaagcgttc gttcagtctg gtaacaaacc
     8941 agagtggatg atcctgaccg ttctgccggt actgccgcca gatctgcgtc cactggttcc
     9001 gctggatggt ggtcgtttcg cgacttctga cctgaacgat ctgtatcgtc gcgtcattaa
     9061 ccgtaacaac cgtctgaaac gtctgctgga tctggctgcg ccggacatca tcgtacgtaa
     9121 cgaaaaacgt atgctgcagg aagcggtaga cgccctgctg gataacggtc gtcgcggtcg
     9181 tgcgatcacc ggttctaaca agcgtcctct gaaatctttg gccgacatga tcaaaggtaa
     9241 acagggtcgt ttccgtcaga acctgctcgg taagcgtgtt gactactccg gtcgttctgt
     9301 aatcaccgta ggtccatacc tgcgtctgca tcagtgcggt ctgccgaaga aaatggcgct
     9361 ggagctgttc aaaccgttca tctacggcaa gctggaactg cgtggtcttg ctaccaccat
     9421 taaagctgcg aagaaaatgg ttgagcgcga agaagctgtc gtttgggata tcctggacga
     9481 agttatccgc gaacacccgg tactgctgaa ccgtgcaccg actctgcacc gtctgggtat
     9541 ccaggcattt gaaccggtac tgatcgaagg taaagctatc cagctgcacc cgctggtttg
     9601 tgcggcatat aacgccgact tcgatggtga ccagatggct gttcacgtac cgctgacgct
     9661 ggaagcccag ctggaagcgc gtgcgctgat gatgtctacc aacaacatcc tgtccccggc
     9721 gaacggcgaa ccaatcatcg ttccgtctca ggacgttgtt ctgggtctgt actatatgac
     9781 ccgtgactgt gttaacgcca aaggcgaagg catggtgctg actggcccga aagaagcaga
     9841 acgtctgtat cgctctggtc tggcttctct gcatgcgcgc gttaaagtgc gtatcaccga
     9901 gtatgaaaaa gatgcaaacg gtgaattagt agcgaaaacc agcctgaaag acacgactgt
     9961 tggccgtgcc attctgtgga tgattgtacc gaaaggtctg ccttactcca tcgtcaacca
    10021 ggcgctgggt aaaaaagcaa tctccaaaat gctgaacacc tgctaccgca ttctcggtct
    10081 gaaaccgacc gttatttttg cggaccagat catgtacacc ggcttcgcct atgcagcgcg
    10141 ttctggtgca tctgttggta tcgatgacat ggtcatcccg gagaagaaac acgaaatcat
    10201 ctccgaggca gaagcagaag ttgctgaaat tcaggagcag ttccagtctg gtctggtaac
    10261 tgcgggcgaa cgctacaaca aagttatcga tatctgggct gcggcgaacg atcgtgtatc
    10321 caaagcgatg atggataacc tgcaaactga aaccgtgatt aaccgtgacg gtcaggaaga
    10381 gaagcaggtt tccttcaaca gcatctacat gatggccgac tccggtgcgc gtggttctgc
    10441 ggcacagatt cgtcagcttg ctggtatgcg tggtctgatg gcgaagccgg atggctccat
    10501 catcgaaacg ccaatcaccg cgaacttccg tgaaggtctg aacgtactcc agtacttcat
    10561 ctccacccac ggtgctcgta aaggtctggc ggataccgca ctgaaaactg cgaactccgg
    10621 ttacctgact cgtcgtctgg ttgacgtggc gcaggacctg gtggttaccg aagacgattg
    10681 tggtacccat gaaggtatca tgatgactcc ggttatcgag ggtggtgacg ttaaagagcc
    10741 gctgcgcgat cgcgtactgg gtcgtgtaac tgctgaagac gttctgaagc cgggtactgc
    10801 tgatatcctc gttccgcgca acacgctgct gcacgaacag tggtgtgacc tgctggaaga
    10861 gaactctgtc gacgcggtaa aagtacgttc tgttgtatct tgtgacaccg actttggtgt
    10921 atgtgcgcac tgctacggtc gtgacctggc gcgtggccac atcatcaaca aaggtgaagc
    10981 aatcggtgtt atcgcggcac agtccatcgg tgaaccgggt acacagctga ccatgcgtac
    11041 gttccacatc ggtggtgcgg catctcgtgc ggctgctgaa tccagcatcc aggtgaaaaa
    11101 caaaggtagc atcaagctca gcaacgtgaa gtcggttgtg aactccagcg gtaaactggt
    11161 tatcacttcc cgtaacaccg aactgaaact gatcgacgaa ttcggtcgta ccaaagaaag
    11221 ctacaaagtg ccttacggtg cggtactggc gaaaggcgat ggcgaacagg ttgctggcgg
    11281 cgaaaccgtt gcaaactggg acccgcacac catgccggtt atcaccgaag taagcggttt
    11341 tgtacgcttt actgacatga tcgacggcca gaccattact cgtcagaccg acgaactgac
    11401 cggtctgtct tcgctggtgg ttctggattc cgcagaacgt accgcaggtg gtaaagatct
    11461 gcgtccggca ctgaaaatcg ttgatgctca gggtaacgac gttctgatcc caggtaccga
    11521 tatgcctgcg cagtacttcc tgccgggtaa agcgattgtt cagctggaag atggcgtaca
    11581 gatcagctct ggtgacaccc tggcgcgtat tccgcaggaa tccggcggta ccaaggacat
    11641 caccggtggt ctgccgcgcg ttgcggacct gttcgaagct cgtcgtccga aagagccggc
    11701 aatcctggct gaaatcagcg gtatcgtttc cttcggtaaa gaaaccaaag gtaaacgtcg
    11761 tctggttatc accccggtag acggtagcga tccgtacgaa gagatgattc cgaaatggcg
    11821 tcagctcaac gtgttcgaag gtgaacgtgt agaacgtggt gacgtaattt ccgacggtcc
    11881 ggaagcgccg cacgacattc tgcgtctgcg tggtgttcat gctgtgactc gttacatcgt
    11941 taacgaagta caggacgtat accgtctgca gggcgttaag attaacgata aacacatcga
    12001 agttatcgtt cgtcagatgc tgcgtaaagc taccatcgtt aacgcgggca gctccgactt
    12061 cctggaaggc gaacaggttg aatactctcg cgtcaagatc gcaaaccgcg aactggaagc
    12121 gaacggcaaa gtgggtgcaa cttactcccg cgatctgctg ggtatcacca aagcgtctct
    12181 ggcaaccgag tccttcatct ccgcggcatc gttccaggag accactcgcg tgctgaccga
    12241 agcagccgtt gcgggcaaac gcgacgaact gcgcggcctg aaagagaacg ttatcgtggg
    12301 tcgtctgatc ccggcaggta ccggttacgc gtaccaccag gatcgtatgc gtcgccgtgc
    12361 tgcgggtgaa gctccggctg caccgcaggt gactgcagaa gacgcatctg ccagcctggc
    12421 agaactgctg aacgcaggtc tgggcggttc tgataacgag taatcgttaa tccgcaaata
    12481 acgtaaaaac ccgcttcggc gggttttttt atgggtgtta ttcagagggc aaattcttca
    12541 cggaaaatat taagaaaggc agggcgaaac gaggaagaag cttttcgccc tggacaacat
    12601 tcctgctgac ggcactacca taaaaaaaca caacgctttc aacaaccttg cgtttttcat
    12661 cttccgtagc ggctcggaat aaatttacgt tcttacattc cgtctcatag tctttgcgag
    12721 gcgcgtccca gatagctgtc ccagtctttc cataccggct gcaaaccctg agcggttaac
    12781 gcggcagcaa ccgcttccgg tctgcgatcg tcgtgcggtg agaactgttc caactcgggg
    12841 tgattatcgg catagccacc tggctgcgtt ttcgagaagg cgctgacgtt attgatcgcc
    12901 agcggaataa cgcgatcgcg aaaccacggt gattcccgcg tggagagtga cagttcaatc
    12961 tccggtgcaa gcagtcggaa ggcgcagatg gtttgcacta actggcgttc atccataatc
    13021 gacgcaggct caatgccgcc agtacacggg cgcaggcgcg gaaaggagac agagtaacgg
    13081 ctttgccagt aatgctgttg cagccatagc aaatgttcgg caaccatata gcagtcaacg
    13141 cgccagttgt cggaaaggcc aattagcgcg ccgaggccta tcttatcaat ccccgcacgc
    13201 cccagccgat ccggcgtttc cagccgccag aagaagtcct gttttttgcc tttcaaatga
    13261 tggcgggtat aagtcgcctc gtgatatgtc tcctgataaa ccatcacccc atccagacca
    13321 agttgcttta actcggcgta ttccgtctcc gccagcggtt gcacttccat ttgtagtgaa
    13381 gagaactgtt cacgcagggc ggggagatga cgacgaaagt aatccatccc cactttcgcc
    13441 tgatgttcac cagtgactaa tagcagatgt tcaaagccca tctcccgtat agcggcgctt
    13501 tccctggcaa tatccgcttc atccagcgtt ttgcgcttga tgcgattact catggaaaat
    13561 ccgcagtacg tgcagtcgtt agcgcaaaga ttggaaagat aaagcgggac gtagaaacta
    13621 actgtgttgc caaatcgctg acgggtcaga cgctgcgccc gttgggccag tggttccaga
    13681 tagccactgg cggctggcga taacagcgcc atcatatcgt cgcgggtgag ttgcgaggca
    13741 tttagcgccc gttctacgtc agcagccgtt ttgccgttga tacgcaggcg gatgtcgtcc
    13801 cagtccagtt gtcgccagcg atcgctgaag gttttcatgc cgatgcctcc agaaatccag
    13861 tcagcgggct ggtggcatga gcaaaatgac tgcggctgcc cggtccggac tgacgtgcca
    13921 gcaggcctgc ttctaccgcc agacgaaatg ccttcgccat gttgacggga tcgtccgcta
    13981 cggcaatcgc cgtattcact aacaccgcgt cggcccccat ttccagggcc tgcgcggcat
    14041 ggctgggaac gccgatgcca gcatcgacaa ccaccggaac tgtggcctgc tggataataa
    14101 tctccagcat ggcacgggtt tccagtccct gattcgagcc aatcggcgcg ccgagcggca
    14161 tcaccgctgc acagccgact tcttccagac gtttacacaa taccggatcg gccccgcagt
    14221 aaggcagcac gacaaatccc tgttgtacca gcatttcggc ggctttcaga gtttcgatgg
    14281 gatcgggcaa cagccagcgg gcgtcagggt gaatctctaa ttttaaccag tttgtgccta
    14341 acgcttcacg tgccagatgg gcggcgaaaa tggcttcttc cgctgttttc gccccggaag
    14401 tatttggcag cagggtcaca cccgccgcga taagcggttc gaggatagcg tcgttgtgct
    14461 ggcgcaagtc gacacgtttc atcgccagtg tcaccagctg gctgccggaa gcgcggatcg
    14521 cctccaccat cagttgtgaa gaagcgaatt tccctgtgcc ggtaaacaga tgtgaatcaa
    14581 acgttttgtc cgcaatacgt aacatctcaa ccccctgcaa taacctgaaa aagcaggatc
    14641 tggtcgccat cctgcacgat atgttgcgtc cactgctcac gcgggacgat ttgctgatta
    14701 atcgccagag ccgcgcccgc ttgtcgttgg tcgagttgct ccagtagttc gtgaacagtt
    14761 tgcccggcgg cgcactgcat cggttgatcg ttaaacagga tctgcattgc ttcctccgca
    14821 taccgggcaa ccactggcgc ggcgcaacgc caggctgcgc cactggctcg atttaccgtc
    14881 gaacagtcgg agttctcccg caggtgtctc tataccgctt aataacttaa tggcttccag
    14941 tgcctgcaaa gtgcccataa ccccgaccac cgggccaacc acgcccgccg tgcggcagtt
    15001 gcgttctggc tcctggttat ctggccacag gcagcggtaa cacccctgct cccagggcgg
    15061 cgtcagtacc atcaactgac cgccaaatcc gaccgcgctg gcggtgataa gcggcgtgtt
    15121 gagtaccacg caggcggcat taatctcctg gcgagtcgcc atattgtcgg tacagtcgag
    15181 caccacatcg gcccgtgcaa ccgcatcttt taacgcctca cccgttaacc gttgttgtaa
    15241 tgctgtcagt tgaatatcgg gattcaactg tgtcagtcgc tgatggctga cctgcgattt
    15301 cgggcgatcg atatcttcag tggtaaagag gatttgtcgt tgcagattgc ttaaatgcac
    15361 atcgtcgtca tctgccagta ccagcgtccc gacgccagcc cccgccaggt atagcgcagc
    15421 aggtgtaccc agcccgccaa gaccaataat cagcacctgg ctgtcgagca gtttttgctg
    15481 cccgtccaga gcgatatcgt cgagcagaat ttggcggcta taacgcataa agtcacggtc
    15541 attcatcgcc aactcctgca atttccagca actgtgccgt tgccaaacgc cagtctgcgg
    15601 cttgagtaat ggcgctgacg acggcgatac tgccgacacc cgttgctatc accgcaggcg
    15661 cgcgcgccag actgataccg ccaatcgcca cggtgggata atccgccagt cgctcaacat
    15721 gccgtgccag ctgttccagc ccctgcggtg cagaaggcat ctgtttggtt tgcgtcggga
    15781 acacatgtcc cagcgcgata taagaggggc gtgctgccag cgcgacgtcg atttccatat
    15841 cgtcatgtgt cgaaacgccc agccgcaggc ctgccgcgcg gatggcattg agatcggtgg
    15901 cttgcaaatc ttcctgcccc aaatggacgc catacgcctg atgcttgatc gccagccgcc
    15961 agtaatcgtt gataaacaat cgcgcgttat agcggcggcc cagcgcaatt gccgccacga
    16021 catcggcttc cacctcttca ttgcgccgat ctttgatgcg tagctggagg gtacgtacgc
    16081 ctgcatccaa cagacgttcg atccactgta cgctgtccac caccgggtac agtcctaaac
    16141 gaaaaggtac aggaggaaaa tcaggctgat acatcacgct tcctccttac gcaggtagat
    16201 ttctccgcct ctggcgcgga agttctccga catatccgcc attcccactt caatggtttg
    16261 cgcggcggcg taatcacgta cttcctggct gattttcatc gagcagaatt tcggcccaca
    16321 catggagcaa aaatgggcga ctttacctga ctcttgcggc agggtttcat cgtgataagc
    16381 gcgggcggta aacgggtcga gggccagatt aaactggtct tcccagcgaa attcgaagcg
    16441 ggctttcgac atggcgttat cgcgaatttg cgcgcctgga tgccctttcg ccaggtcagc
    16501 ggcgtggacg gcaatcttat aggtgataag cccctgctta acatcttctt tattaggcag
    16561 acccagatgc tcttttggcg ttacgtaaca gagcatcgcg cagccaaacc agccaatcat
    16621 cgccgcacca atccccgacg tgaagtggtc atagcccggc gcaatatcgg tagttagcgg
    16681 ccccagagtg taaaacggcg cttcgtggca gtgctctaac tcctcggtca tattgcggcg
    16741 gatcatctgc atcggcacgt ggcctgggcc ttcaatcatc acctgcacgt catattccca
    16801 ggcaattttg gtcagttcgc ccagcgtatg cagctcggca aactgcgctt catcgttggc
    16861 gtcctgaata gaaccggggc gcagaccgtc gcccagcgac agcgaaacgt cataagcggc
    16921 acagatttca caaatttcgc ggaagtgttg atagaggaaa ttttcctgat gatgggagag
    16981 gcaccatttc gccataatcg aaccgccgcg agagacgata ccggtcaggc gtttcgcggt
    17041 catcggcaca tagcgcagca gtacgcccgc atggatagtg aagtaatcca caccttgctc
    17101 ggcctgttcc agcagcgtgt cgcggaacgc ttcccaggta agatcttcgg cgatcccgtt
    17161 aaccttctcc agcgcctggt agatcggcac tgtaccgatc ggcaccgggc tgttacgcaa
    17221 aatccactcg cgggtttcgt gaatatagcg accggtggag agatccatca ccgtatccgc
    17281 tccccagcgc gtggaccata ccagcttttc cacttcttct tcgatggaag aggtgaccgc
    17341 cgagttgccg atattggcgt taacttttac caggaaattg cgaccaataa tcatcggctc
    17401 cgattccgga tgattaatgt tggccgggat aatcgcacgt ccggcagcaa cttcatcacg
    17461 gacaaattcc gcagtgatat tttccggcag atgtgcgcca aagctcattc ccggatgctg
    17521 gtggcgtaaa acctcgctac ggatgcgctc gcggcccata ttctcgcgga tggcgatgaa
    17581 ttccatttcc ggcgtgatga tgccctggcg ggcgtagtgc agttgggtga cacggcgtcc
    17641 tgttttggcg cgttttggcg ttaataagcc gctaaaacgt agctcgtcga gaccatcatc
    17701 tgccagccgc gttctggtgt aatcggaact gcgcacggta agttcttcgg tatcgccgcg
    17761 cgcatcgatc cacggctggc gtagttttgc cagcccttgc tgcacgttaa tggcgatctg
    17821 cggatcacca tacgggccgg aggtgtcgta gaccggaatc gcttcgtttt cttcgtactg
    17881 cggctgttct ttgctaccgc caattagcgt cgggctaagc tggatctcac gcatcggcac
    17941 gcgcacgccg gggtgtgtgc cagtgatata aatgcgtttt gagttgggaa aggcggtgcc
    18001 ttccagggtg tcgataaaat gttgggcccg ggcgcgttgt tcgcggcggg tcagttttgt
    18061 tgcagacata gctcattcca aaaagtcaag gacgtggctt gtcagacgac ggatgaagca
    18121 agagacgatc gccgcaaagg cgatgcgata gcagattaac tcttgttccc ttcgcaggta
    18181 ttagcctgat caggttccgc ggatcccgaa taaacggtct cagccagtta aggcactccg
    18241 acaagaaatt agcccccaca aacggggcgt tgaatgtaaa ttacgcgtta acagcgcaga
    18301 actcaagcag gatgtttgac gcgggcggcg tcaagcacca gcagaatcag cttatcttcc
    18361 agcacaaagc gcgcttccag cgcctcgccg atgtcagata aaacctgttg aaactcaagg
    18421 taattatcat gatcgatggc ggtttccagg ctggaatcgt agtaatccat aatctgttgg
    18481 gtattggctt cgagttgcgg ccaaatcttc gcggcgcgcg cgagttgccc gttgccttcc
    18541 agcttatgaa gaatgcgctc ataaatactg aaatgtccgg cagacaagta atcgaccagg
    18601 ctctgacaaa aatcatcaag ggctttttcg tttagcctca tgtacgattc tttgccaggc
    18661 ttaatgccaa ccagattgta gtaagccacg agcagatgct tacgtacatg tagccagcga
    18721 tcaaccagtt tgttacttcc tctgacgcgc tccgtcaggt tatcgagctg gttaagcatg
    18781 attgactccg caagtttgta ttcaaaaact gctcagtgag aaatgtaaaa accatgttaa
    18841 acatgccagt gatgcaaagg tagtgcaaga gctatggatc gtataattga aaaattagat
    18901 cacggctggt gggtcgtcag ccatgaacaa aaattatggt tgccgaaggg agaattgcca
    18961 tatggcgaag cggcaaattt cgatcttgtg ggtcagcgcg cactacaaat cggcgaatgg
    19021 cagggggaac ctgtttggtt agtacaacag cagcggcgtc acgatatggg gtcggtacgt
    19081 caggtcattg atctcgatgt tgggctgttt caactggccg gacgaggcgt acaactggcg
    19141 gagttttacc gatcgcataa atactgtggt tactgcgggc atgaaatgta tccgagcaaa
    19201 accgaatggg cgatgctgtg cagccattgc cgtgagcgtt actacccgca aatcgccccc
    19261 tgcattattg ttgccatccg tcgcgatgat tcgatcctcc tcgcccagca tacccgccat
    19321 cgtaacggtg tccatacagt acttgccgga ttcgtcgaag tgggcgaaac cctcgagcag
    19381 gcagtcgcgc gggaagtgat ggaagagagc ggaattaaag ttaaaaactt gcgttacgtg
    19441 acttctcagc cgtggccgtt tcctcagtct ttaatgaccg cgtttatgac ggaatatgac
    19501 agcggcgaca tcgtgatcga cccgaaagaa ttgctcgagg cgaactggta tcgctatgac
    19561 gatttgccgt tactcccgcc gcccggcacc gtagcgcgcc gtctgataga agatactgtg
    19621 gcgatgtgtc gggcagagta tgagtgatga tacactgacc gcctgacgca ctaaggaaca
    19681 gccaaaatga ccgaacttaa aaacgatcgt tatctgcggg cgctgctgcg ccagcccgtt
    19741 gatgtcactc cagtatggat gatgcgccag gcgggtcgct atctaccgga atataaagcc
    19801 acgcgcgccc aggcgggcga ttttatgtcg ctgtgcaaaa acgccgagct ggcgtgcgaa
    19861 gtgactttgc aaccgctgcg tcgctacccg ctggatgcgg cgatcctctt ttccgatatc
    19921 ctcaccgtgc cggacgcgat ggggttaggg ctctattttg aagccggaga aggtccgcgt
    19981 tttacctcgc cagtcacctg caaagccgac gtcgataaac tgccaattcc ggacccggaa
    20041 gatgagctgg gttacgtgat gaacgcggtg cgtaccattc gtcgcgaact gaaaggcgaa
    20101 gtgccgctga ttggtttttc cggcagcccg tggacgctgg cgacctacat ggtggaaggc
    20161 ggcagcagca aagcgttcac cgtgatcaaa aaaatgatgt atgccgatcc gcaggcgctg
    20221 cacgctctac tcgataaact ggcgaaaagc gtcactttgt atctgaatgc gcagattaaa
    20281 gccggtgctc aggcagtgat gattttcgac acctggggcg gtgtgcttac cgggcgcgat
    20341 tatcaacagt tctcgctcta ttacatgcat aaaattgttg atggtttact gcgtgaaaac
    20401 gacggtcgcc gcgtgccagt cacgctgttt accaaaggcg gcggacagtg gctggaagcg
    20461 atggcagaaa ccggttgcga tgcgttgggc ctcgactgga caacggatat cgccgatgcg
    20521 cgccgccgtg tgggcaataa agtcgcgttg cagggtaata tggatccgtc gatgctgtac
    20581 gcgccgcctg cccgcattga agaagaagta gcgactatac ttgcaggttt cggtcacggc
    20641 gaaggtcatg tctttaacct tggtcacggc attcatcagg atgtgccgcc agaacatgct
    20701 ggcgtgttcg tggaggcagt gcatcgactg tctgaacaat atcaccgcta aggagtgatt
    20761 atggatctcg cgtcattacg cgctcaacaa atcgaactgg cttcttctgt gatccgcgag
    20821 gatcgactcg ataaagatcc accggatctg atcgccggag tcgatgtcgg gtttgagcag
    20881 ggcggagaag tgacgcgagc ggcgatggtg ctgctgaaat atccctcgct tgagctggtg
    20941 gagtataaag ttgcccgcat cgccaccacc atgccttaca ttccaggttt tctttccttc
    21001 cgcgaatatc ctgcgctgct ggcagcgtgg gagatgctgt cgcaaaagcc ggatttagtg
    21061 tttgtcgatg gtcatgggat ctcgcatcct cgccgtcttg gcgtcgctag ccattttggt
    21121 ttgatggtgg atgtgccaac gatcggcgtg gcgaaaaaac ggctctgcgg taaattcgaa
    21181 ccgctctcca gcgaaccggg cgcgctggcc ccactgatgg ataaaggcga gcagctggcc
    21241 tgggtctggc gcagcaaagc gcgctgtaac ccgttgttta tcgctaccgg ccatcgggtc
    21301 agcgtggaca gcgcgctggc gtgggtacaa cgctgcatga aaggctatcg tctgccggag
    21361 ccaacgcgct gggcggacgc ggtggcctcg gaacgtccgg cgttcgtgcg ctatacagca
    21421 aatcagccct aattcaggta aactgcggat aatttccgta tttgagaact caacatgtta
    21481 caaaacccaa ttcatctgcg tctggagcgc ctggaaagct ggcagcatgt cactttcatg
    21541 gcttgcttat gcgaacgcat gtaccccaat tacgccatgt tctgccagca aaccggtttt
    21601 ggtgatgggc aaatttaccg tcgtattctc gatctcatct gggaaacgct gaccgttaaa
    21661 gacgcaaaag taaatttcga cagccaactg gagaaatttg aagaagcgat tccttcagcc
    21721 gacgatttcg atctgtacgg cgtttatccg gcaatcgatg cctgcgtggc gttaagtgaa
    21781 ctggtccatt cgcgtttgag tggtgaaacg ctcgaacacg cggtggaagt gagtaagacc
    21841 tccatcacga ccgttgcgat gctggaaatg actcaggctg gtcgcgaaat gagcgatgaa
    21901 gagcttaaag aaaacccagc tgtagagcaa gaatgggaca ttcagtggga aatattccga
    21961 cttttagctg agtgcgaaga acgcgatatc gagctgataa aaggccttag ggcagacctg
    22021 cgtgaggcgg gtgagagcaa tattggtata atttttcagc aataagacca gaaaacgtga
    22081 tttaacgcct gatttgtcgt acctggagtc ttccctttcg ccccccgtct ggtctacatt
    22141 tggggggcga aaaaaagtgg ctatcggtgc gtgtatgcag gagagtgcta ttctggcatt
    22201 tccgtcgcac tcgatgctta gcaagcgata aacacattgt aaggataact tatgaacaag
    22261 actcaactga ttgatgtaat tgcagagaaa gcagaactgt ccaaaactca ggctaaagct
    22321 gctctggagt ccactctggc tgcaattact gagtctctga aagaaggcga tgctgtacaa
    22381 ctggttggtt tcggtacctt caaagtgaac caccgcgctg agcgtactgg ccgcaacccg
    22441 cagaccggta aagaaatcaa aattgccgca gctaacgtac cggcatttgt ttctggcaag
    22501 gcactgaaag acgcagttaa gtaagattgc gtggcagtga acagttttaa cgaaggggtg
    22561 gtttcacccc ttttgtcttt ctggcgtcga tcattgatgc tggctggcgc tctgcttctc
    22621 actgcctgta gtcataactc ttcacttcct ccctttaccg ccagtggatt tgctgaagac
    22681 cagggcgcgg tacgcatctg gcgaaaagac agcggcgata atgtgcatct gcttgccgtg
    22741 tttagcccgt ggcgcagtgg cgataccacg acgcgagagt atcgctggca gggcgataac
    22801 ctcacgctca tcaatatcaa tgtttacagc aaaccgccgg tgaatattcg cgcgcgtttt
    22861 gacgatcgcg gtgatctgag ctttatgcaa cgtgaatccg atggggaaaa gcagcagctt
    22921 tctaacgacc aaatcgattt ataccgttat cgtgctgatc agatccgcca gattagcgat
    22981 gccttacgtc aggggagagt cgtgctgcgc caggggcgct ggcatgcgat ggaacagacc
    23041 gtgaccacct gcgaagggca aaccattaaa cctgatttag attcgcaggc gatagcgcat
    23101 atcgagcgcc gccagagccg ctcttctgtt gatgtcagcg tggcatggct ggaagcgccc
    23161 gaaggttcgc aattactgtt agtggcaaac tctgatttct gtcgctggca acccaacgag
    23221 aaaacgttct gatttaccag tggcccatac ccatatgacc gccaccaccg cagccgccgt
    23281 agcccatgcc cattccggct ccgcgcggaa tacccgcttc agccatcgcg atatctcgtt
    23341 tcacccgtaa ctcatctaac gactgacgca aattctccat ctctttggcg accgcgttaa
    23401 ttttgctgct atccggtggg ttcgcggcta acagggcatt gtattcataa cgcttcgtca
    23461 ccagttgctg ttgcagtgcg ctgctttgag cgtaaaagtc attatggatt ttctgccacg
    23521 ctgtctgttg ttcgctggtc aaaggcgcgg cattttgctg ccacataccg tgtccgccgt
    23581 gagcaaatgc agatgtcgat cccatcgcca ttgctgaaag cgccatcatt accagggcaa
    23641 ttttcgtgtt ccgtttcatg gttaatcctc cagtggttgt cttacttcgg gtattgcatc
    23701 ttccgtgcca acgatgaaac gctgatatga cgggtaatct ggcatgataa acgagtaaaa
    23761 atgactcgcc tgctgcgggt agcgagtcat ttttactcat tgaaacttaa gcctttgtgt
    23821 tacagcgcag ggtaagcgct gataaaagat ggcatgattt ctgctgtcag aaagggatga
    23881 gcaggcaaag aagaagatgc gttttatgca acgttctaaa gactccttag ctaaatggtt
    23941 aagcgcgatc ctccccgtgg tcattgttgg gctggtaggg ttgtttgcgg tgacggtgat
    24001 tcgcgattat gggcgcgaga ctgccgccgc cagacaaacg ctgctggaaa aaggcagtgt
    24061 acttattcgc gctcttgaat ccggctcgcg cgtcggcatg gggatgcgca tgcatcatgc
    24121 gcagcagcag gccttactgg aagaaatggc cgggcagcct ggagtacgtt ggtttgcggt
    24181 cacggatgaa caaggaacaa tcgtgatgca tagcaactcc ggcatggtgg gaaaacagct
    24241 ttattccccg caggaaatgc agcagttaca tccgggagat gaagaagcgt ggcggcggat
    24301 cgatagcgca gacggcgagc ctgttctgga aatttatcgc cagtttcaac cgatgtttgc
    24361 tgctggaatg caccggatgc gccatatgca gcagtatgcc gcgacaccac aagcaatttt
    24421 catcgctttc gacgccagta atattgtgag tgccgaagat cgtgagcaga gaaacaccct
    24481 gattatcctc ttcgccctgg cgacggtctt gctggcaagc gtattgtcat tcttctggta
    24541 tcgccgctat ttgcgctcgc gccagcttct acaagatgaa atgaagcgca aagagaagct
    24601 ggtggcgctg gggcatcttg cggcaggcgt tgcccacgaa atccgtaacc cactttcctc
    24661 gattaaagga ctggcgaaat actttgccga gcgcgcgcct gcagggggag aagcgcatca
    24721 actggcgcag gtgatggcga aagaggccga ccgtttaaac cgcgtggtaa gcgagctgct
    24781 ggaactggtt aagccaacgc atctggcttt gcaggcggtg gatctcaaca cgctgattaa
    24841 ccactcatta cagctggtaa gccaggatgc gaacagccgg gagatccagt tacgctttac
    24901 cgccaacgac acattaccgg aaattcaggc cgatccggac aggctgactc aggtcctgtt
    24961 gaatctctat ctcaatgcta ttcaggcgat tggtcagcat ggcgtgatta gcgtgacggc
    25021 cagcgaaagc ggcgcgggcg tgaaaatcag cgttaccgac agcggtaagg gaattgcggc
    25081 agatcagctt gatgccatct tcactccgta cttcaccact aaagccgaag gcaccggact
    25141 ggggctggcg gtcgtgcata atattgttga acaacacggt ggtacaattc aggtcgcaag
    25201 ccaggaggga aaaggcgcaa cgttcaccct ctggcttccg gtcactatta cgcgtaaggg
    25261 cccacaagga tgacgcacga taatatcgat attctggtgg tggatgatga cattagccac
    25321 tgcactattt tgcaggcttt actgcgcggc tggggctata acgtcgcgct ggcgaacagc
    25381 gggcgacagg cgctggagca ggtgcgggaa caggtttttg atcttgtgct ttgcgatgtg
    25441 cgaatggcgg agatggacgg cattgccacg ctaaaagaga tcaaagcgtt aaatccggcg
    25501 attccggtgc tgattatgac cgcatactcc agcgtcgaga cggcggtaga agcgttgaaa
    25561 accggggcgc tggattatct catcaagccg ctggatttcg ataacctgca ggcgacgctg
    25621 gaaaaggcgc tcgcgcatac gcacagtatt gacgctgaaa cgcctgcggt gtctgccagc
    25681 cagttcggta tggtcggtaa aagcccggcg atgcaacacc tgctcagtga aatcgccctc
    25741 gtcgcgccat cggaagccac ggtgctgatc cacggcgatt ccggcaccgg taaagagctg
    25801 gtcgccaggg cgattcacgc cagtagcgca cgtagcgaaa aaccgctggt aacgctcaac
    25861 tgtgcggcac tcaacgaatc cttgctggaa tctgaattgt tcggtcacga aaaaggggcg
    25921 ttcactggag ccgatagacg ccgggaaggg cgctttgttg aggcggacgg cggcacgctg
    25981 tttctcgatg aaattggcga tatctcgccg atgatgcagg tgcgtctgct gcgtgcgatt
    26041 caggagcgcg aagttcagcg tgtcggtagc aaccagacta tctcggttga tgtccggttg
    26101 attgcggcga cccatcgcga tcttgccgca gaggtgaatg ccgggcgttt tcgccaggat
    26161 ctctactatc gcctgaatgt ggtggcgatt gaagtaccat cgctgcggca acggcgggaa
    26221 gatatccctc tgctggctgt ccattttctt cagcgctttg ccgagcgtaa tcgcaaggcg
    26281 gtaaaaggct ttacgccaca ggcgatggat ttgctgatcc actacgactg gccgggaaat
    26341 attcgtgaac tggaaaacgc ggtggaacgg gcggtggtgc tgctgaccgg ggaatatatt
    26401 tccgaacgcg agctgccgct ggcgattgcc ggtacgccga tcccactggg gcaaagtcag
    26461 gatattcagc cgctggtgga agtcgaaaaa gaagtgattc tggcggcact ggagaaaacg
    26521 ggcggcaaca aaaccgaagc cgcccgtcag ttagggatca cgcgcaaaac gctattggca
    26581 aaactgtcgc gttagttctg ctcgcgttcg atagcgcgcc agccgatatc tttccggcag
    26641 aagcagtcgt cccagtgaat atcggtcatt aaggcataag cgcgtttctg cgcttctgcc
    26701 acggtatgac ccagcgcggt gacgcacagt acgcgcccgc cgctggtcac tacctgctcg
    26761 ttatccgcca gtttcgtgcc cgcgtggaac actttgccgc ctgccacttc ttccagcggc
    26821 aggccgtgga tcacatcgcc ggtgcggtaa tcgcccggat atccacccgc agccatcacc
    26881 acgccgagag aagcacgctc atcccactct gacgttttct cgtccagttt gccttcacag
    26941 gccgccaggc agagctcaac cagatcggac ttcatgcgca gcataatcgg ctgggtttct
    27001 ggatcgccaa agcggcagtt aaattcgata accttcggat tgccctgttt gtcgatcatc
    27061 aggcccgcgt agagaaaacc ggtgtaggtg ttgccttccg ccgccatgcc tttcacggtt
    27121 ggccagatga tacgttccat ggtgcgctga tgaacttcat cggttactac cggcgcgggg
    27181 gagtaagcgc ccatcccgcc cgtgtttggt ccggtatctt tatcgcctac gcgtttgtga
    27241 tcctggctgg tggccatcgg cagcacatgc tcgccgtcta ccatcacgat aaagctcgct
    27301 tcttcgccgt cgaggaactc ttcgataacg atgcgatgac ccgcgtcgcc aaaagcgttg
    27361 cccgccagca tatcgtgaac agccgcttcg gcttcttcca gcgtcatcgc cacgataacg
    27421 cctttcccgg ctgccagacc gtccgcttta atgacgatcg gcgcaccttt ctcacgcaga
    27481 tacgccagcg caggttctac ctcggtgaag ttctggtatt ccgctgtagg gatcttatgg
    27541 cgggccagga aatctttggt aaacgctttc gaaccttcca gttgcgcggc acctgcggtt
    27601 gggccgaaga ttttcatccc ggcggcgcgg aaggtatcga ccacgccttt caccagcggc
    27661 gcttccgggc cgacgatggt cagatcaatc ttttcgtttt gtgcgaaatc caacagcgcc
    27721 gggatatcgg tcacgccgat agcaacgttt tgcagcgcgg gttccagcgc agtgcctgca
    27781 ttacccggag caacaaaaac agtttcaacc agcggcgact gcgccgcttt ccaggccagc
    27841 gcgtgctcgc gcccgccgtt accaatcact aatactttca tctattgctc cattaatggc
    27901 ggaagtggcg catgtcggtg aagagcatcg caataccgtg ctcgtcggcg gcggcaatca
    27961 cttcgtcatc acggatagaa ccgccaggct ggattacgca ggtcacgcct gcagcggcgg
    28021 cggcatcaat accgtcgcgg aacgggaaga acgcgtcaga agccatcgag gaacctttca
    28081 cttccaagcc ttcatcggca gctttaatac cggcgatttt cgcggagtac acgcggctca
    28141 tctggcccgc gccaatcccg atggtcatat tgtttttggc atagacgata gcgttggatt
    28201 tcacgaactt cgccactttc cagcagaaca gcgcatcacg cagttcctgt tcggtcggct
    28261 gacgttgggt gacgacgcgc agttcttccg caccgaccat gcccaggtca cgatcctgaa
    28321 ccagcagacc gccgttcacg cgtttgaaat caagacccgg aacacgctcg ccccactgac
    28381 cgcaggtcag aacgcgtacg ttctgcttgg cggcggtgat tttcagggct tcttcgctgg
    28441 cggaaggcgc aataatcact tcgacaaact gacgagaaat gatggcctgt gcggtttccg
    28501 catccagctc gcggttaaag gcaatgatgc cgccgaatgc ggaggttggg tcggttttgt
    28561 acgcgcgatc gtaagcatca agaatggaat tgccgatagc cacgccgcaa gggttggcgt
    28621 gcttcacaat cacacatgcc ggctcggcga actctttcac gcactccagc gccgcatcgg
    28681 tatcggcgat gttgttataa gagagggctt taccctgaac ctgggttgcg gtagcaacgg
    28741 aggcttcttt cacattctct tctatataga aggcagcctg ctggtggctg ttctcgccgt
    28801 aacgcatatc ctgcttctta atgaagttca ggttcagcgt gcgtgggaag cgaccggcgg
    28861 cttctttgct ttcaccgtgg taagccggaa ccatgctgcc gaagtagttg gcaatcatgc
    28921 tgtcgtaggc ggcagtgtgt tcgaaggctt tgatggcgag gtcgaaacgg gttgcaagcg
    28981 tcagcgatcc ttcgttgtca tccatctctt taataatggc gtcgtagtcg ctgctcttca
    29041 ccacgattgc gacatcttta tggttcttag cggcggagcg caccatggtc gggccgccga
    29101 tatcgatgtt ctcaaccgca tcttccagcg agcagccttc acgagcaaca gtctgggcga
    29161 acggatacag gttaacaacc accatatcga taggctggat ctgatgttct tccataatgg
    29221 catcgtcctg gccgcgacgg cccagaatgc caccatgtac tttcggatgc agggtcttca
    29281 cgcgtccatc catcatctcc gggaaaccgg tgtaatcgga aacttcggtt accggcagac
    29341 ctttttctgc taacagacgg gcagtgcccc ctgtagacag cagctccaca ccgcgtgcgg
    29401 aaagtgcctg ggcgaattcg acgataccgg ctttgtcaga aacactgagc agagcgcggc
    29461 ggactggacg acgttgttgc atggtaaatc ccctggattt gactattaca gagagcgtta
    29521 gctgaatttt tcgcgaaaaa ctcagctaac gcccctaacg gggcatcctt atttttcgcc
    29581 cgcattgtaa cgaaaacgtt tgcgcaacgc tcgcgaattt ttctctttca atggtgatca
    29641 caattttgac agtggttacc gtgggaaaaa tacagaaatt acattgatga ttgtggataa
    29701 ctctgtgcgt aaaaaggtat aaagcggggt tttgctgggg aatgcagcag tcagtcattt
    29761 ttctgcaatt tttctattgc ggcctgcgga gaaatcccta taatgcgcct ccatcgacac
    29821 ggcggatgtg aatcacttca cacaaacagc cggttcggtt gaagagaaaa atcctgaaat
    29881 tcagggttga ctctgaaaga ggaaagcgta atatacgcca cctcgcgaca gtgcgctaaa
//
LOCUS       DAJTZL010000033        29882 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.033, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000033 DAJTZL010000000
VERSION     DAJTZL010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29882
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.033"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            417..1865
                     /gene="uacT"
                     /locus_tag="OO544_001301"
     CDS             417..1865
                     /gene="uacT"
                     /locus_tag="OO544_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HCR6766938.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKDAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPFIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFLHTSFSQNVGLVSVTRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
     gene            1867..1992
                     /locus_tag="OO544_001302"
     CDS             1867..1992
                     /locus_tag="OO544_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6766939.1"
                     /translation="MNFLMRVIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(1989..2060)
                     /locus_tag="OO544_001303"
     CDS             complement(1989..2060)
                     /locus_tag="OO544_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6766940.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            2115..2663
                     /gene="idi"
                     /locus_tag="OO544_001304"
     CDS             2115..2663
                     /gene="idi"
                     /locus_tag="OO544_001304"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HCR6766941.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTRLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESNEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPNGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLHGIDATPWAFSP
                     WMVMQAANSEARKLLSAFAQHN"
     gene            complement(2706..4223)
                     /gene="lysS"
                     /locus_tag="OO544_001305"
     CDS             complement(2706..4223)
                     /gene="lysS"
                     /locus_tag="OO544_001305"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HCR6766942.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(4233..5331)
                     /gene="prfB"
                     /locus_tag="OO544_001306"
     CDS             complement(join(4233..5255,5257..5331))
                     /gene="prfB"
                     /locus_tag="OO544_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HCR6766943.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            complement(5422..7155)
                     /gene="recJ"
                     /locus_tag="OO544_001307"
     CDS             complement(5422..7155)
                     /gene="recJ"
                     /locus_tag="OO544_001307"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HCR6766944.1"
                     /translation="MKQQIQLRRRAVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGETLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEADKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            complement(7161..7871)
                     /gene="dsbC"
                     /locus_tag="OO544_001308"
     CDS             complement(7161..7871)
                     /gene="dsbC"
                     /locus_tag="OO544_001308"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HCR6766945.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     ALQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKEMKEFLDEHQKMTSGK"
     gene            complement(7896..8792)
                     /gene="xerD"
                     /locus_tag="OO544_001309"
     CDS             complement(7896..8792)
                     /gene="xerD"
                     /locus_tag="OO544_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HCR6766946.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            8904..9425
                     /gene="fldB"
                     /locus_tag="OO544_001310"
     CDS             8904..9425
                     /gene="fldB"
                     /locus_tag="OO544_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HCR6766947.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            complement(9465..9872)
                     /locus_tag="OO544_001311"
     CDS             complement(9465..9872)
                     /locus_tag="OO544_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin CptA"
                     /protein_id="HCR6766948.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(9853..10119)
                     /gene="sdhE"
                     /locus_tag="OO544_001312"
     CDS             complement(9853..10119)
                     /gene="sdhE"
                     /locus_tag="OO544_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HCR6766949.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            10362..11342
                     /gene="ygfZ"
                     /locus_tag="OO544_001313"
     CDS             10362..11342
                     /gene="ygfZ"
                     /locus_tag="OO544_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HCR6766950.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDEHVLLGVAGFQARAALANIFSELPSKEKQVVKEGATTLLWFEYPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            complement(11538..12197)
                     /locus_tag="OO544_001314"
     CDS             complement(11538..12197)
                     /locus_tag="OO544_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HCR6766951.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(12361..12672)
                     /locus_tag="OO544_001315"
     CDS             complement(12361..12672)
                     /locus_tag="OO544_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HCR6766952.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLTELIKVIADIYPGQTQFYVIEFKC
                     L"
     gene            12717..14150
                     /gene="bglA"
                     /locus_tag="OO544_001316"
     CDS             12717..14150
                     /gene="bglA"
                     /locus_tag="OO544_001316"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HCR6766953.1"
                     /translation="MKKLTLQKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDDMFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHENPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYCCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVITSNGEKL"
     gene            complement(14207..14950)
                     /locus_tag="OO544_001317"
     CDS             complement(14207..14950)
                     /locus_tag="OO544_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR6766954.1"
                     /translation="MAIALVTDGSRGIGRATALLLAKEEYTVAVNYQQNLHAAQEVVN
                     LITQAGGKAFVLQADISDENQVIAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNMTGYFLCCREAVKRMALKNGGSGGAIVNVFSVASRLGSPGEYVDYAASK
                     GAIDTLTTGLSLEVAAQGVRVNCVRPGFIFTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(15216..18089)
                     /gene="gcvP"
                     /locus_tag="OO544_001318"
     CDS             complement(15216..18089)
                     /gene="gcvP"
                     /locus_tag="OO544_001318"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HCR6766955.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAAVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            complement(18208..18597)
                     /gene="gcvH"
                     /locus_tag="OO544_001319"
     CDS             complement(18208..18597)
                     /gene="gcvH"
                     /locus_tag="OO544_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HCR6766956.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            complement(18621..19715)
                     /gene="gcvT"
                     /locus_tag="OO544_001320"
     CDS             complement(18621..19715)
                     /gene="gcvT"
                     /locus_tag="OO544_001320"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HCR6766957.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPADRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVLEGI
                     GETAIVQIRNREMSVKVTKPVFVRNGKAVA"
     gene            20005..20088
                     /locus_tag="OO544_001321"
     CDS             20005..20088
                     /locus_tag="OO544_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6766958.1"
                     /translation="MSKNTKSKNNDIRKYNAKTEVKLVYFK"
     gene            complement(20162..21364)
                     /gene="ubiI"
                     /locus_tag="OO544_001322"
                     /gene_synonym="visC"
     CDS             complement(20162..21364)
                     /gene="ubiI"
                     /locus_tag="OO544_001322"
                     /gene_synonym="visC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HCR6766959.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLVLVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGTNPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(21387..22565)
                     /gene="ubiH"
                     /locus_tag="OO544_001323"
                     /gene_synonym="visB"
     CDS             complement(21387..22565)
                     /gene="ubiH"
                     /locus_tag="OO544_001323"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HCR6766960.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADYATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLESGETLTGRVLVA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTRAARA
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            complement(22562..23887)
                     /gene="pepP"
                     /locus_tag="OO544_001324"
     CDS             complement(22562..23887)
                     /gene="pepP"
                     /locus_tag="OO544_001324"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HCR6766961.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNELEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIMNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            complement(23913..24491)
                     /gene="ygfB"
                     /locus_tag="OO544_001325"
     CDS             complement(23913..24491)
                     /gene="ygfB"
                     /locus_tag="OO544_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HCR6766962.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            24659..24988
                     /gene="zapA"
                     /locus_tag="OO544_001326"
     CDS             24659..24988
                     /gene="zapA"
                     /locus_tag="OO544_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HCR6766963.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            25030..25213
                     /gene="ssrS"
                     /locus_tag="OO544_001327"
     ncRNA           25030..25213
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="OO544_001327"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            25234..25836
                     /locus_tag="OO544_001328"
     CDS             25234..25836
                     /locus_tag="OO544_001328"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HCR6766964.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(25937..25996)
                     /locus_tag="OO544_001329"
     CDS             complement(25937..25996)
                     /locus_tag="OO544_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCR6766965.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(26328..26387)
                     /gene="ibsC"
                     /locus_tag="OO544_001330"
     CDS             complement(26328..26387)
                     /gene="ibsC"
                     /locus_tag="OO544_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HCR6766966.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            complement(26616..27848)
                     /gene="serA"
                     /locus_tag="OO544_001331"
     CDS             complement(26616..27848)
                     /gene="serA"
                     /locus_tag="OO544_001331"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HCR6766967.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            complement(28104..28763)
                     /gene="rpiA"
                     /locus_tag="OO544_001332"
     CDS             complement(28104..28763)
                     /gene="rpiA"
                     /locus_tag="OO544_001332"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HCR6766968.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            29193..>29882
                     /gene="argP"
                     /locus_tag="OO544_001333"
     CDS             29193..>29882
                     /gene="argP"
                     /locus_tag="OO544_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017374530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HCR6766969.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQL"
BASE COUNT         7110 a         8049 c         7497 g         7226 t
ORIGIN      
        1 tgagttatgt cttctcaaat ttcgcgatgc aataaacggg catatattag gtagcatgac
       61 cgttttaatt ataattttgc gactgagttc aaattattgc ccctcaatct atgccgcttt
      121 cattatttca acattgccga tcaaaagagc gctatccaga taaaaacgat taaatgcgag
      181 agtgcgacat gccagaatga ttaatgaaat atcaatatga gaaaattaca gataattcca
      241 ttgtcaggtt ctcttgtcat tttcgtggtt taagcacgat tatgtgattg cctccgcaat
      301 tcctgtctct aactcccctt cctcgcaaaa actggcactc cacgagcatg tgtttagaca
      361 gtttcattaa cgtaaacggt tgctttttac tctggcgggc gaaaggagaa acactgatga
      421 gcgccataga ttcccaactt ccctcatctt ctgggcaaga ccgcccaact gatgaggttg
      481 accgcatatt atcaccagga aagctgatca tactcggtct gcaacacgtc cttgtcatgt
      541 acgcaggtgc agtcgctgtt cctcttatga ttggtgaccg actgggcctc tcaaaagatg
      601 ctattgcgat gctcattagc tcggatctct tttgctgcgg gatcgtcaca ttattgcaat
      661 gtatcggtat cggccgcttt atggggatcc gcctgccggt gattatgtcg gtgacctttg
      721 ctgctgtaac accaatgata gccattggga tgaacccgga tatcggcctg ctggggatat
      781 ttggtgccac tatcgccgcg ggttttatca ccacattatt agcgccattt attggtcgct
      841 tgatgccttt attcccgcca ctggttaccg gtgtggttat tacttctatt gggcttagca
      901 tcattcaggt gggtattgac tgggccgccg gaggtaaagg gaatccgcaa tatggtaatc
      961 ccgtttattt aggtatctcc tttgctgtct taatttttat cttgctcatt actcgctatg
     1021 cgaaaggatt tatgtccaac gtcgccgtat tactggggat tgtatttggc tttttacttt
     1081 cgtggatgat gaatgaagtc aatttatccg ggctacatga tgcttcatgg tttgcgattg
     1141 tcacgccgat gtcatttggt atgccgattt tcgatcccgt ttccattctg accatgactg
     1201 ccgtgttaat catcgtgttt atcgagtcga tggggatgtt cctggcactg ggtgaaatag
     1261 tcggtcgtaa actctcttca cacgatatta ttcgcgggct gcgtgtcgat ggcgtaggga
     1321 caatgatagg cggaacgttt aacagcttcc tccacacgtc attttcacaa aacgttggcc
     1381 tggttagcgt gacgcgcgtt catagccgct gggtgtgtat ttcttcggga attatattaa
     1441 tcctgtttgg catggtgcca aaaatggcgg tgctggtcgc ctccattccg caatttgtgc
     1501 tgggcggtgc tgggctggtg atgttcggca tggtactggc gacagggatt cgaattctgt
     1561 cgcgctgtaa ctacaccacc aaccgttaca acctctatat tgtggcgatc agtctcggcg
     1621 ttggcatgac tccgacgctc tctcacgatt tcttttctaa gttaccggcc gtactgcaac
     1681 cgttgctgca tagcggcatt atgctcgcaa cccttagcgc cgttgtgctg aatgtcttct
     1741 ttaatggcta ccagcatcat gctgacctgg tgaaggaatc cgtctctgat aaagatttaa
     1801 aagtcaggac agtacgtatg tggcttctga tgcgcaagct gaagaaaaat gagcatggag
     1861 aataatatga attttttaat gcgcgttata ttcagtctgc tgttgctttt tactctctct
     1921 attcctgtca tttctgactg tgttgcaatg gccattgaaa gtcgcttcaa atatatgatg
     1981 ctactttttt aaatggtttt tacctgtcgg catccgctca aaacgggcgg ttgtcgataa
     2041 acgctcactt ggttaatcat ttcactcttc aattatctat aatgatgagt gatcagaatt
     2101 acatgtgaga aattatgcaa acggaacacg tcattttatt gaatgcacag ggagttccca
     2161 cgggtacgct ggaaaagtat gccgcacaca cggcagacac ccgcttacat ctcgcgttct
     2221 ccagttggct gtttaatgcc aaaggacaat tattagttac ccgccgcgca ctgagcaaaa
     2281 aagcgtggcc tggcgtgtgg actaactcgg tttgtgggca cccacaactg ggagaaagca
     2341 acgaagacgc ggtgatccgc cgttgccgtt atgagcttgg cgtggaaatt acgcctcctg
     2401 aatctatcta tcctgacttt cgctatcgcg ccaccgatcc gaatggcatt gtggaaaatg
     2461 aagtgtgtcc ggtatttgcc gcacgcacca ccagtgcgtt acagatcaac gatgatgaag
     2521 tgatggatta tcaatggtgt gatttagcag atgtattaca cggtattgat gccacaccgt
     2581 gggcgttcag tccgtggatg gtgatgcagg cagccaatag tgaagcaaga aaattgttgt
     2641 ctgctttcgc gcagcacaat taaaaaaccc cgacatttgc cggggttgtg agcataacgt
     2701 aatgcttatt ttaccggacg catcgccggg aacagaataa cgtcgcggat ggtatggctg
     2761 ttggtgaaca gcattaccat acggtcgatg ccaatcccca gacctgctgt cggcggtaag
     2821 ccatgttcca gtgcggtaac gtaatcttca tcgtagaaca tcgcttcgtc gtcacctgcg
     2881 tctttcgcgg caacctgatc caggaagcgt tgcgcctgat cttccgcgtc attcagctcg
     2941 ctaaagccgt taccgatttc acgaccaccg atgaagaact caaagcggtc tgtgatttcc
     3001 gggttaatgt cgttacgacg cgccagcgga gaaacttctg ccggatattc agtaatgaag
     3061 gtcggctgaa tcagatgtgc ttctgccact tcttcgaaga tctcggtaac gatacggccc
     3121 agaccccagc tcttctcaac gtggataccg atagattcag caatcgcttt cgcagagtcg
     3181 aagttatcca gatccgccat gtcggtttcc gggcgatatt tcttgatcgc ttcacgcatg
     3241 gtcagttttt cgaacggttt accgaagtcc agcgtcacgt cgccgtaggt cacttccgtc
     3301 ttaccgagaa tatcctgtgc cagagtacgg aacagcgatt cggtcagctc gatcagatct
     3361 ttgtaatctg cgtaagccat gtagagttcc atcatggtga actctgggtt atgacgtacg
     3421 gaaatacctt cgttacggaa gttacggttg atttcgaata cacgctcgaa gccaccgacc
     3481 accagacgct tgaggtacag ttccggcgcg atacgcaggt acatgtcgag atccagcgcg
     3541 ttatggtggg tgataaacgg acgcgcagcg gcaccgcctg ggatcacctg catcatcggc
     3601 gtttcaactt ccataaagcc gcggttcacc atgaactggc gaataccaga gaggatctgc
     3661 gagcgtactt taaaggtgtt acgggattca tcgttagaga tgagatccag ataacgctga
     3721 cgatagcgcg cttcctgatc ctgcaagccg tggaatttat ccggcagcgg acgcagtgct
     3781 ttggtcagca gacgcagctc ggtacagtgg atagacagtt cgccggtttt ggttttgaac
     3841 agtttacctt tcgcgccgag gatgtcgccg aggtcccatt ttttgaactg ctcgttgtaa
     3901 acgccttccg gcagatcgtc acgggcaacg tacagctgaa tgcggccacc cacatcctgc
     3961 agggtaacga aagacgcttt acccataata cggcgggtca tcatgcggcc agcaacggcg
     4021 acttcgatgt tcagcgcttc cagttcttcg ttctctttac cgtcgaattc tgcgtgcaat
     4081 tggtcagagg tatgatcgcg acggaaatcg ttcgggaagg caatcccctg ctcgcgcagg
     4141 ttcgccagct tctcacgacg cgttttcagt tcattgttaa gatcgactac cgcgtcagcg
     4201 ccctgtgcgt gttgttcaga catgttggtt cctcataacc ctgctttcaa acttgcttcg
     4261 ataaattgat ccaggctgcc gtccagcacg gcctgcgtgt tgcgagtttc taccccggta
     4321 cgcagatctt taatgcggga gtcatcaagg acataagaac gaatctggct gccccagccg
     4381 atgtcagatt tgttatcttc catcgcctgt ttctcggcat ttttcttctg catctccagt
     4441 tcataaagct tcgctttcat ctgcttcatg gcctgatctt tgttcttgtg ctgggaacgg
     4501 tcgttctggc actgggtcac gatcccggtc gggatgtggg taatacgcac cgcagattcg
     4561 gtacggttaa cgtgctgacc gcccgcgccg gacgcgcgat aaacgtcaat gcgcagatcc
     4621 gccgggttga tttcgatatc aatatcatca tcaacttccg gataaacaaa cgcagagctg
     4681 aacgacgtgt ggcgacgacc gccggagtca aacgggctct tacgcaccag gcggtgaacg
     4741 ccagtttctg tacgcagcca gccgtaagcg taatcgccgg agattttgat cgtcacggat
     4801 ttaatacccg ccacttcacc ttccgactct tcgatgattt cagttttgaa accacgcgat
     4861 tctgcccagc gcagatacat acgctcaagc atgctcgccc agtcctgtgc ttccgtaccg
     4921 ccagaccccg cctgaatatc gaggtagcag tcggcgctgt catattcgcc agagaacata
     4981 cggcggaact caagctgcgc cagtttttct tccagggcgt cgagttcagc aacggcttcg
     5041 ttaaaggttt cttcgtcgtc agcttctaca gccagttcca gcagaccaga aacatcttcc
     5101 agcccctgtt tcatttggtc gagggtgtcg acaacggctt cgagggagga acgctcttta
     5161 cccagcgcct gtgcgcgttc gggttcattc cagacatccg gctgttccag ctcggcgttt
     5221 acttcttcca gacgctcttt cttggcgtcg tagtcaaaga taccccctaa gaacgtcgga
     5281 gcgttccgtg aggtcctgaa tgcgattatt taccggatta atttcaaaca tggtctgatt
     5341 tcttttattg agctagtcaa aatgcggtga taagagcggg attgtaccca atccacgctc
     5401 ttttttatag agaagatgac gctaaattgg ccagatattg tcgatgataa tttgcaggct
     5461 gcggttgccg cgaaactcgt tgatatcgag cttgtaagcc agttgcactt cgcgcacgcc
     5521 gttatccggc cagagggcgg tatcgacatt aaaagcaata ccatccagca gcggaccgcc
     5581 gccgaccggt tcgaccatga ctttcaaatg acgttcgccc accagccgct gttgcagcag
     5641 ccggaaatga ccgtcaaaca gcggctccgg gaacatctgc ccccacgggc cagcatcgcg
     5701 cagcagctgc gccacttcca tggtcatttc ggccgggctt aacggaccgt ctgacaccac
     5761 ttcgccttgc aatagcgaag ggtccagcca ctcagtaacc agttcgccaa accgttgttg
     5821 aaagagttcg aatttatccg cttccagcga caaacccgcc gccatcgcat gaccgccaaa
     5881 cttcagcatc atgccagggt agagtgtgtc taatcgctcc agcgcatcac gcatatgcag
     5941 cccctgaatg gagcgaccag aacctttcag cgtaccatca cccgcgggcg caaaggcgat
     6001 aaccggacgg tgaaaacgct ctttaatacg ggaagccaga ataccgacaa ccccctgatg
     6061 ccattcgggg tgatacattg ccagcccgcc gggtagcgtg tcacggctgc gctccagttt
     6121 ctcgcacagg gtcagggctt caacctgcat tccttgttcg atctcttttc gcgtctggtt
     6181 tagcgcatcg agttcatttg ccagcacgcg cgcttcgccg atgttgtcgc acagcaagag
     6241 cgccacaccg acggacatat cgtccagtcg tccggcagca ttgagacgtg gccccagcgc
     6301 aaaacctaaa tcgctggcgg cgagtttttg tgcatcacgg tttgccactt caagcagcgc
     6361 tttaatcccc ggacggcact ttccggcacg gatgcgactc atcccctgcc aggtcagaat
     6421 gcgattatta gcgtccagcg gtacgacgtc cgccactgtc cccagcgcta ccagatccag
     6481 cagttctgcc aggttaggaa ttgcgatgcc gcgctcatca aaccagccct gatcgcgcaa
     6541 aaaggtgcgc agcgccagca tcagataaaa cgccacaccc acgcctgcca gtgatttcga
     6601 cgggaaatta cagtcgcgca agttagggtt aatgatcgct tccgccgcgg gtaatgtttc
     6661 gcccggcaaa tggtgatcgg taacaataac cggaatgccc aacgagcgag cgtgttcaac
     6721 ccccgcatgg gaggaaatac cgttatccac cgtgacaatt aactgcgcac cacgggcatg
     6781 ggcctgatcg accacttccg ggcttaagcc gtaaccgtct tcgaaacggt ttggaaccag
     6841 gtagtcgata ttgctgcaac caagcgagcg catcgccagc acgcttagag ccgtgctggt
     6901 ggcaccgtcg gcgtcgaaat caccgaccac aataatccgc gttccttcgc gaaaggcgtt
     6961 gtaaaggatc tcaacggcct tttcgacgcc gctcagttgc tgccagggca gcatcccttt
     7021 aacactgcgt tccagttctt gcgcactgcg cacgccccgg ctggcgtata aacggcgcag
     7081 caagggaggc aattcagcgg gcaagtctgc cgtttcatcg actgcacggc gacgaagttg
     7141 tatctgttgt ttcacgcgaa ttatttaccg ctggtcattt tttggtgttc gtcgaggaat
     7201 tctttcatct ctttcggcgg ctggtaaccc ggaacaagtg tgccattgct cagcacaact
     7261 gccggagtac cgctaacgcc aagctggacg ccaagtgcgt aatggtcggc aatatccacg
     7321 tcgcagctgg ctggtgcgac gcttttacct gccatcacat catcaaacgc tttgttttta
     7381 tctttcgcac accagatagc tttcatttct ttctctgcat cgctgtccag cccctggcgc
     7441 gggaaagcaa gataacgcac ggtgatcccc agcgcgttat agtctgccat ttgctcatgc
     7501 agtttgtggc agtaaccaca ggtaatatca gtaaacacgg tgatgacgtg tttttcctgc
     7561 agcgctttat aaacgatcat ctctttttca agcgcattca actgctttaa cagcatctta
     7621 ttggtgacat tgaccggagc cgtgccgcta acgtcataca ttggcccctg aatgatatgt
     7681 ttaccatcat cggtgatgta caacacgccg ctgttagtca gaactgtctt catgccagct
     7741 acgggcgcgg gctgaatatc gctgcttttg atgcccattt tggctaacgt ttgttgaatt
     7801 gccgcgtcat cagcctgagc aaaacctgaa aacgccgcta acaaagtaaa caacataaaa
     7861 cctttcttca taaatcttcc cgttcttttc agacatcacg cccgcgggtg atgctgttga
     7921 tgaagttgcc gcagacgctc ggtagcgaca tgcgtataaa tttgcgtggt ggaaagatcg
     7981 ctgtggccca gtagcatctg caccacgcgt aaatccgcac catgatttaa taaatgagtg
     8041 gcaaaagcgt gacgcaacac atgcggtgac agcttttcgc tgtcgatacc cgccagcacg
     8101 gcataatgtt taatgcggtg ccagaaggtc tgtcgcgtca tctgctgcgc acgctggctg
     8161 ggaaacaaca cgtcaattga cacaccattc agcagccacg gacgcccatg ttccagatag
     8221 gtttccagcc agtaaaccgc ctcttcacct aacggtacca ggcgctcttt gttgccttta
     8281 ccaatgaccc gcactacgcc ctgacgcagg ctgatatcac tcattgtcag cccgaccagt
     8341 tcagagacac gcagcccggt agcatacaac acttcaagca tggctttatc gcgtagctcc
     8401 agtggctgat caattaatgg cgcctgtaat aaacgttcga cctgcgcttc gcttaaatct
     8461 tttggcaaac gctggggcaa tttcggtgaa gcgagatgcg cactgggatc gtcttcacga
     8521 aacttttcgc gataaagata ctggaacaat cggcgcactg cactcagcaa acgcgctgag
     8581 ctggtggctt tatacccgcc ctccagccgt tctgccagta atgcctgcaa atcgtcactt
     8641 tgcgccgtcg ccagcgtcaa cccgcggtga tgcaaccact ccaccatcat tgacagatcg
     8701 cgacggtaag cgttcaacgt attttcagcc agattttttt ccagccacag agcatcaaga
     8761 aactgctcga tgcgtgccag atcctgtttc acttgcgccc cttatggtca ctcatttgat
     8821 ccattatgcc ttattgtgcc gtgactaaag cgattctgat acactagctg caaaagccac
     8881 agcagaatcg agaagcttac gttatgaata tgggtctttt ttacggttcc agcacctgtt
     8941 acaccgaaat ggcggcagaa aaaatccgcg atattatcgg cccagaactg gtgaccttac
     9001 ataacctcaa ggacgactcc ccgaaattaa tggagcagta cgatgtgctc attctgggta
     9061 tcccgacctg ggattttggt gaaatccagg aagactggga agccgtctgg gatcagctcg
     9121 acgacctgaa ccttgaaggt aaaattgttg cgctgtatgg gcttggcgat caactgggat
     9181 acggcgagtg gttcctcgat gcgctcggta tgctgcatga caaactctcg accaaaggcg
     9241 tgaagttcgt cggctactgg ccaacggaag gatatgaatt taccagcccg aaaccggtga
     9301 ttgctgacgg gcaactgttc gtgggtctgg cgctggatga aactaaccag tatgacctta
     9361 gcgacgagcg tattcagagc tggtgcgagc aaatcctcaa cgaaatggca gagcattacg
     9421 cctgatctca ctgacggctt agcgcatatg ctttgccgtc atctttatct ttgcgtctct
     9481 tgttgcaaca aaatccgccg taaatcccgc cattcggctt cgtccatgct gtcggctgcc
     9541 agccataaat gttgccgttt accgccatca gaacgtaaac gcagcatcat gccgctctta
     9601 atcatccacg gtgctttgac gatgctccac tcctgccctt gccaacgcaa acgcccgtcc
     9661 atcaacaagc gaatttcccc ctggcgagca ttaatacgcc gctggctgcg aacgcaatca
     9721 aacaccacca gcgaaagtaa caccatccat aacggggtgt aactgagtgg ccagggcatg
     9781 agtaaaataa cagcggcaac cagcccatga atcagcaagg aaagccactg tgcgcgccag
     9841 gagacgcgca aatcagattg ccacaggacc acgttcccgg ttccgtgtct ggatgagtcg
     9901 gaccatcatt tccagttctg catcggccgg tttaccgtga ttcatcagcc agttaaacag
     9961 gtccggatcg tcacattcca gcagacgaat aaagatgcgt ttttcgtcat cgcttaagct
    10021 gtcgtactca tgttcgaaaa acggcatgat tgaaatatcg agttcgcgca taccacggcg
    10081 gcatgcccaa tgaatgcggg ctttgttgtt aatgtccatc ttcttcctgt ctcacgaaaa
    10141 tccagtaccc cgctattgta acgtgttttt cgccttcttt tacgggaata tcagtaaaca
    10201 ccatcgcgat cacgaaatta attcacaaca attcaatggc ttcatttttt tggaagtcgc
    10261 ctcgcagaag gcacagatcg cgtagtgaaa gcacttgcat tgcctcatag ctcttttacc
    10321 attagtcatt aatacgccgt taagcaactc aggactctat tatggctttt acaccttttc
    10381 ctccccgtca gcctacggct tctgcccgtt taccgctgac gctgatgacg cttgatgact
    10441 gggcgcttgc caccattact ggcgcggaca gcgaaaaata tatgcagggt caggtgacag
    10501 cagatgtcag ccagatggca gaagatcagc acctgctcgc cgcccattgc gacgccaaag
    10561 gcaaaatgtg gagcaattta cgtctgttcc gcgacggcga tggctttgcg tggattgaac
    10621 ggcgcagcgt gcgtgaaccg cagctgactg aactgaaaaa atatgcggtg ttctctaaag
    10681 tgaccatcgc gccagacgac gagcatgtgc tgcttggtgt tgccggtttt caggcgcgcg
    10741 ccgcgctggc aaatatcttt agtgaactgc cttcgaaaga aaaacaggta gttaaagaag
    10801 gcgcgaccac tttgctatgg tttgaatacc cggcagaacg tttcctgatc gtaaccgatg
    10861 aagctactgc taatatgctg accgataaac tgcgcggtga agcggaactg aacaatagcc
    10921 aacagtggct ggcattaaac attgaagcgg gtttcccggt gattgatgcc gccaacagcg
    10981 ggcagtttat cccacaggcg accaatctcc aggcgctggg cggtatcagc tttaagaaag
    11041 gctgttatac cggacaagag atggtggcgc gagcaaaatt ccgtggtgcc aataaacgtg
    11101 cgctctggtt gctggcaggt agcgccagcc gactgccgga agctggtgaa gacttagagc
    11161 tgaaaatggg cgagaactgg cgtcgtaccg gtacggtgct ggctgcggta aaactggaag
    11221 atggtcaggt cgtggtacag gtcgtcatga ataacgatat ggaaccggat agcatcttcc
    11281 gcgtacgcga cgatgcgaat acattgcata ttgagccgct gccgtattcg ctcgaagagt
    11341 aaatctcttt atcgcatcag gcatttatcg cctgatgcga cgctgacgcg tcttatcatg
    11401 tctgggattt gatgccttta tcacaacagg gacgaggccc gtagatcgga taaggcgttc
    11461 acgccgaatc cggcagttgt gctccgatgc ctgatgcgac gctgacgcgt cttttcaggc
    11521 ctacaaaggc atacccatta cgcctgccca atatacaaat agatcgccag aaagtggcac
    11581 acactgccgc cgagcacgaa gccgtgccag atggcatgat tgtagggaat gcgtttgcag
    11641 acatagaaaa tcactccgag cgaataaacc acgccgccta ccgccaataa ggtaacgctg
    11701 cccgccgcga gcttaactgc catttcataa attaccacca gcgacagcca gcccatcgcc
    11761 agataggtca ccagagataa aattttgaat cggtgcgcga tggtcagttt aaacagaata
    11821 cccagcaatg ccaggctcca gataacaatc atcaacccgc gcgctaacgg agaatccagc
    11881 cccaccagca aaaacggcgt gtaggttccg gcaatcaaca ggtaaatagc gcaatggtca
    11941 aatttcttca gccacatttt tgcccgttga tgaggaatgg cgtgatagag cgtcgaagcg
    12001 aggaacagca ggatcatact gccgccatag aggctgtaac tggttatcgc tgtggcgctg
    12061 gcattgagat ccaccgcctg aaccagtagc aacaccagcc caacgatacc aaacaccaac
    12121 ccaatgccgt gactgacgct gttggcaatt tcctctgcca gcgaatatcc ctgcttaatg
    12181 aggggcttct gaaccataac ttactccgga gaaacgtaca cgcacatgta tacctctcca
    12241 gcgtaactga gaatagttcc agtgaacacc tgttagctaa aataaattct gatttgagac
    12301 taatctccta aaaatcatga aattaaatgc gaaatttcaa ctaacaggcg tgagttcaat
    12361 ttaaagacat ttaaattcaa tcacataaaa ctgtgtctga ccgggataga tgtcggcaat
    12421 gacctttatc agttcagtca gggtcatatt ttcctgctct gcatgttttt ccgtcagcgt
    12481 atccagcgtt acggttgagg ttgcggtgac ttcaatcgtg caaaaataac cgtcatcttc
    12541 aaaacgcccg acacgaagca catcacccgt tttgaagtgc gattcagact cgtcgcggat
    12601 ggtgatggtt ttacgcccag ccagaatgtc atcctggaaa cgttgaaaaa aagtgatgtc
    12661 gtttggctgc atggtactat ttcctgtaag aattgactca tctggagcct atgatagtga
    12721 aaaaactcac cttacagaaa gatttcttat ggggcggcgc agttgccgct catcaggtcg
    12781 aaggcggctg gaacaaaggc ggcaaagggc cgagcatttg tgacgttctg accggtggcg
    12841 cacacggcgt gccgcgcgaa atcaccaaag aagtcttgcc aggcaaatac tatccaaacc
    12901 atgaagccgt tgatttttat ggtcactaca aggaagacat caagctattt gccgaaatgg
    12961 gcttcaaatg ttttcggact tccatagcct ggacgcgcat ttttccaaaa ggcgatgaag
    13021 ctcagccaaa cgaagaaggg ctgaagttct acgatgatat gttcgatgaa ctgctgaaat
    13081 acaacatcga accggtgatc accctctccc actttgaaat gccgctgcat ctggtgcagc
    13141 aatacggtag ctggaccaac cgtaaagtgg ttgatttctt tgtccgtttc gcggaagtgg
    13201 tgtttgaacg ctataagcat aaagtcaaat actggatgac cttcaacgaa attaacaacc
    13261 agcgtaactg gcgtgcaccg ctgttcggtt actgctgctc cggcgtggtt tataccgagc
    13321 atgagaaccc ggaagagacg atgtatcagg tgctgcatca ccagtttgtc gccagcgccc
    13381 tggcggtgaa agctgcgcgt cgcattaacc cggagatgaa agtcggctgt atgctggcga
    13441 tggtgccgct ctatccctac tgctgtaacc cggacgatgt gatgttcgct caggagtcga
    13501 tgcgcgaacg ctacgtcttt accgatgtgc agctacgcgg ctattacccg tcctatgtgt
    13561 tgaacgagtg ggagcgtcgc ggatttaaca tcaaaatgga agacggcgat ctcgatgtac
    13621 tgcgcgaagg cacctgcgat tatcttggtt tcagctatta catgaccaac gcagtgaagg
    13681 ccgaaggcgg caccggcgat gcgatctctg gtttcgaagg cagcgtaccg aacccgtatg
    13741 ttaaagcgtc tgactggggc tggcagattg atccagtagg tctgcgctat gccctttgcg
    13801 aactgtatga gcgttatcag aggccgctgt ttattgtcga aaacggtttt ggcgcttacg
    13861 acaaagtgga agaagatggc agcatcaacg acgactaccg cattgactac ctgcgcgccc
    13921 atatcgaaga gatgaaaaaa gcggtgactt acgatggcgt ggatctgatg ggctacacac
    13981 cgtggggctg catcgactgc gtatcgttca ccaccgggca gtacagcaaa cgctacggct
    14041 ttatctatgt gaataaacat gacgacggta ctggcgatat gtcgcgttca cgtaagaaga
    14101 gctttaactg gtacaaagag gtgattacca gcaacggcga gaagctttaa gtcgatgaag
    14161 taccggatgc aatacttgtt gcatccggtc agacaacttc cctgttttat ttcccgcccg
    14221 ccaaatcgat aaaacttccc gtgacgtaag aggctttatc acttagtagc cagacaatgg
    14281 cctgcgcgac ctcttctgcc tgtccaccac gctgcatggg gatgttcgac ttaacgcgat
    14341 cgacgcgtcc aggctcgccg ccgctggcgt gcatttcggt aaaaataaac cctggccgca
    14401 cgcagttaac gcggaccccc tgcgcggcga cttccagcga tagtccggtg gttaacgtat
    14461 caatcgcccc tttcgatgcc gcgtaatcaa catattcccc tggcgaaccc aaccgtgagg
    14521 ccaccgaaaa gacattgacg atagcgccgc cactgccacc atttttaagc gccatgcgtt
    14581 ttaccgcctc gcggcagcag agaaaatatc ccgtcatgtt ggtggaaagt actcggttga
    14641 ttcgctctgc ggtaaggttt tcaacggtgc actgggtaaa caagatcccg gcgttattga
    14701 ccagcgctgc tagcggttca tcgtgctgat cgattgctgt aaacattgcg atgacctgat
    14761 tttcgtcgct gatatccgcc tggagcacga atgccttgcc accggcttgc gtgattaagt
    14821 tcaccacttc ctgcgccgca tggaggtttt gttgataatt aaccgccacc gtatactctt
    14881 ctttcgccaa cagtaatgca gttgcccgcc cgatgccgcg actgccatcg gtcacaagtg
    14941 ctatagccat ttgtttttcc gagaaaaatt aattcgttga cgtatctttg agattgctaa
    15001 caaactggct caatcttcaa tgttggcacg cttttcccct cgccctttca gggagagggc
    15061 cggggtgagg gtaaatattc gcgccagtgc cggcctgttc ccctcaccct aaccctctcc
    15121 ccaaaggggc gaggggactg tctgagcact tttgtacttt gtcatctgac taaaaaggcg
    15181 ccgaagcgcc tttagaaaat agtcgaatca gtgaattact ggtattcgct aatcggtacg
    15241 caggagcaga acaggttacg gtcgccgtaa acatcatcca gacgtttcac cgtcggccag
    15301 tatttgtctg ccacgcctgc cgggaatacc gcaacttcac ggctgtacgg atgcgcccac
    15361 tcggcgacca gttcgctctg aatgtgcggc gcgttcacca gcgggttatc ttccagcggc
    15421 cagacagcgg ctttcacctg gtcaatttct gcgcggatag ccagcatcgc gtcgataaag
    15481 cgatccagtt ccactttgct ttcagattca gtcggttcaa ccatcagcgt acccgccacc
    15541 gggaacgaca tcgtcggcgc gtggaaaccg tagtcgatca ggcgcttggc aatatccagc
    15601 tcgctgatgc cggtttcttc tttcagcggg cgaatatcga gaatacattc gtgcgccacg
    15661 cgaccgtcgc gaccggtata cagcaccggg aaggcatcct gcaggcggct ggcaatatag
    15721 ttggcgttga ggattgccac ctggcttgct tttttcagcc cttctgcgcc catcatgcgg
    15781 atgtacatcc agctgattgg caggatagag gcgctaccga acggtgccgc agaaactgcg
    15841 ccctgacggg ttaacatgcc ttcgatttgc accacgctat gacccggtac aaacggtgcc
    15901 agatgcgctt tcacaccgat cggtcccata cccggaccac caccgccgtg cggaatgcag
    15961 aaagttttat gcaggttgag gtgcgaaacg tccgcgccaa taaagcccgg cgaggtgatg
    16021 ccaacctggg cgttcatgtt cgcgccatca aggtaaacct gaccgccgaa ctgatgcacg
    16081 acttcacaca cttcacggat cgtttcttca tacacgccgt gggtagaagg ataagtcacc
    16141 atgatacagg agaggttatc gcccgcctgt tccgctttcg cgcgcagatc agtcagatcg
    16201 atgttgccgt ttttatcaca cgccacaacc accacctgca ttcctgccat atgtgcagaa
    16261 gcggggttag ttccgtgcgc agaagccggg atcaggcaga tatcgcgatg cccttcgttg
    16321 cggctttcat gataatgacg aatcgccagc aggcccgcgt attcgccctg tgcgccggag
    16381 ttcggctgca tacaaacggc gtcgtaaccg gtcagtttca ccagccagtc agccagctgc
    16441 gcaatcatct gctgataacc ttcagcctgt tccggcgggc aaaacgggtg cagttcggca
    16501 aattccggcc aggtgattgg gatcatctcg gcggcggcgt tcagtttcat ggtgcaggaa
    16561 cccagcggga tcatcgcctg attcagcgcc agatctttac gctccagcga gtgcatatag
    16621 cgcatcattt cggtttcgct gtggtagcga ttaaacaccg gatgggtgag gatttcgtcg
    16681 tcgcgcagca tcgcaggctg gatagagcgg ctgtcgtgag ccacgtcttt gtccagcgtg
    16741 tcgatctcca gcccgtggtt atcgcccagc agcacgctga aaagctgcat tacgttttca
    16801 cgcgtggtgg tttcatcaag ggtgatcccc acggcgttca ggatatcgct acgcaggttg
    16861 atttcagccg cttcggcacg cgtcagtacg cccgctttgt cggccacttc cacacacaag
    16921 gtgtcgaaat agtgcgcatg gcgcagtttc agaccttttt gttgcaggcc cgccgccaga
    16981 atatcggtca gacggtgaat gcggttagcg atacgtttca ggccaaccgg gccgtgataa
    17041 acggcataca ggctggcgat gtttgccagc agtacctggg aagtacaaat gttggagttc
    17101 gctttctcac gacgaatatg ttgctcgcga gtctgcatcg ccatgcgcag cgcggtattg
    17161 ccagctgcat ctttcgatac accgataata cggcccggca ttgagcgttt gtattcatct
    17221 ttcgccgcaa agaatgccgc gtgtgggcca ccgtagccca tcggcacgcc gaagcgttgc
    17281 gccgaaccaa aaacaatatc cgcgccctgt ttacccggcg cagttaacag caccagcgcc
    17341 ataatatcgg cggcaacgct gaccacaatt ttgcgtgatt tcagttcgct aataagcgca
    17401 gtgtagtcgt gaatttcacc ggtagtgcct acctgctgta acagcacgcc gaagacgtcc
    17461 tgatggtcga gcactttttg cgcgtcatcg acaatcactt caaaaccaaa ggtttcggca
    17521 cgagtacgga ccacatccag cgtttgcgga tgcacatcgg aagccacgaa gaagcggttg
    17581 gcatttttca gtttgctgac gcgtttcgcc atcgccattg cttcggcggc agcggtggcc
    17641 tcgtccagaa gagaagcaga ggccatatcc agtccagtca aatccagcgt tacctgctgg
    17701 aagttgagca gtgcttcaag gcggccctgg gagacttcag gttgatacgg agtgtacgcg
    17761 gtataccagc ccggattttc cagcatgtta cgcaggataa ccggcggtag ctgcacggcg
    17821 gtgtaaccca tgccgatgta agacgtgaag cgtttattgc gactggcaat agccttgagt
    17881 tctgccagtg cggcgtattc ggtcgccggt gcgccaacct gcggcggggt cgcaagctga
    17941 atatctttcg gtacaatctg gccggtcagc gcgtttaacg attgtgcgcc aacggcattc
    18001 agcatttctt gctgttgcgc ggcgtccggt ccgatatggc gttcaataaa agcgccgctg
    18061 ttttcaagct ggcttaacgt ctgtgtcatg agcgatggtt cctgaaacgt gcagtgaatt
    18121 gtgaacctct ctccttacga agagagtgag ggtgaggcat aaattttcct caccctgatc
    18181 ctctcccgca gaagaggaat aaagccgtta ctcgtcttct aacaatgctt cgtatgcggt
    18241 cgcatccagc agtgattcca gttcgctttc atcgctggct ttgattttaa agatccagcc
    18301 acctgcatac ggttcgctgt tcaccagttc cggggaatcg ctcagtgcgt cgtttaccgc
    18361 cacgatttca ccgcttactg gcgcataaat gtctgacgcc gcttttactg attcggcaac
    18421 cgcgcagtca tcgcccgcgc taaccgttgc gcccacttcc ggcaggtcaa caaacaccat
    18481 atcgcctaac agctcctgag catgttcggt aataccaacg gtgtaagtgc cgtcggcttc
    18541 tttacgcagc cattcgtgtt ctttgctgta tttcagttct gctggtacgt tgctcatcaa
    18601 tcaatctcca aaaaagtaaa tcacgcgacg gctttgccgt tacgcacaaa aacaggtttt
    18661 gtcactttaa ccgacatttc acggttgcga atttgcacaa tcgccgtttc gccaatacct
    18721 tccagcacgc gcgccagcgc aatgctgtaa cccagcgtcg gggagaaagt accgctggtg
    18781 ataatgcctt catgctggtt gccctgcgca tcggtaaagc gtaccggcag ttcattacgc
    18841 agcacgcctt tttcggtcat caccagacca accagttttt ctgtaccatg ctcacgctgc
    18901 acttccaggg cttcacgacc gataaagtca cgatctgccg gttcccaggc gatagtccag
    18961 cccatgttgg cggctaaagg agaaatagtt tcgtccatct cctgaccata aagattcatg
    19021 cccgcttcca gacgcagcgt gtcacgcgcg cccaagccac atggcttaac acccgcttcc
    19081 accagcgcac gccagaaatc ggccgctttt tcattgggca gcgcaatttc atagcccgct
    19141 tcaccggtat aaccagtggt ggcaataaac agatcgcccg cctgcacgcc aaagaacggt
    19201 ttcatccctt ccaccgcctg acgctgggcg tcattaaaca gtgtggcagc ttttgcctgc
    19261 gcattcggcc cttgcacggc aatcatggaa aggtcatcac gaacggtaat ttcgatgccg
    19321 aaaggttcag cgtgttgggt aatccaggag aggtcttttt cgcgggtggc ggagttaaca
    19381 acgaggcgga agaaatcttc agtaaagtag tagacgatga ggtcatctat cacaccgcca
    19441 gaggcattca acatccccga gtaaagggct ttgccgcttt tggtgagctt cgccacatcg
    19501 ttcgccagca gataacgcag aaactcccgg gtgcggctgc cgcggagatc gacgatggtc
    19561 atatgtgaca catcaaacat tccggcatcg gtacgtaccg catgatgttc gtcgatttgc
    19621 gaaccgtaat gcagcggcat catccagccg tggaaatcca ccatgcgagc gccgcaaagc
    19681 gtgtgttgtt cgtacaaagg agtctgttgt gccatcttgt cctcattgaa taagcggggc
    19741 tgacaacttt tcatggtgaa attatcacca cgaaacccag catcggagcc actcccggtc
    19801 cccaacgcaa tcgttctctt ttgcctgaac ttaccaccga aacagactgt taaccataag
    19861 gtaaaattga tcatcacatt agcttatggt taaaaaacgc caaaatcgcg acagaataaa
    19921 aaaccaaaaa acacaccagt ttctatataa agatgatgcc attagaaagc taatttgaat
    19981 aaggcaatat taagagctaa aaaaatgtca aaaaacacta aatcaaaaaa taatgacatt
    20041 agaaaatata atgcgaaaac ggaggtgaaa ttagtttatt tcaaatgagg aaaatctccc
    20101 ggcgaaaaaa ccgggagatg aaagtgtgat gggtatcaaa taaacagcag aagagaaatt
    20161 tttaacgcag ccattcaggc aaatcgttta atcccattgc ctggcggata agttgtggtt
    20221 taacgccagg aagcgtgtcg gccagtttca aaccaatatc acgcagcagt tttttcgccg
    20281 gattggtacc ggaaaacaga tcgcggaatc cctgcatacc agccagcatc agcgccgcac
    20341 tgtgcttgcg gctacgctca taccggcgca ggtaaatgta ctgcccgatg tctttcccct
    20401 gacgatgcaa ccgtttcagt tcagcaatca gctctgcagc atccataaag ccaagattta
    20461 ctccctgccc cgccagcggg tgaatggtat gcgcggcgtc gcccaccagc accagacggt
    20521 gcgcggcaaa ctggcgcgca taacgccccg tcagtgggaa cacctgacgc gcgctctcaa
    20581 ccttgcataa gcccaggcga ttatcaaaag cgatatttaa cgcgcgatta aattcatctt
    20641 cacttgcctg ctgcatccgc tgcgcttcct ctggcgacag tgaccagaca attgagcaaa
    20701 gatgcggatc gctaagcggt aaaaaggcca gaatgccttc gccatggaaa acctgccgcg
    20761 ccaccgcatc atgcggttct tccgtgcgaa tggtcgctac cagcgcgtga tgctgataat
    20821 cccagaaagt cagcggaata tctgctttgt tgcgcaacca ggaattagca ccgtccgcgc
    20881 caatcaccag acgcgccgtt aacatgctgc catctttcag cgtcaggaaa gtttcatttt
    20941 ctccccaggc gacctgctgt aattctgcgg gggccaacag agtgatatct gacgactgct
    21001 gcgctttgtt ccacagcgcg tagtgaatca ctgagttttc aacgatatgc ccaagatggc
    21061 tatagcccat gctttgatcg tcaaacgaaa tgtgaccaaa actgtctttg tcccacactt
    21121 ccataccgtg ataacaactg gccctacgag agagaatgtc ctgccagacg ccaagacggg
    21181 tgagtaattt ttcgctggcg gcattgatag ccgaaacgcg cagttgtggt ggtgcatccg
    21241 ccgccagagg ttccggtacg cgctgctcca gtacggcaac gcgtaagccg ctcccctgta
    21301 agccgcaggc aaccgccagc cccaccatgc cgccaccaac aatggctaca tcaacacttt
    21361 gcattgttta ttccttaaaa ccgccttcaa cgcgccaccc aaccgagggt gcgctgcgcc
    21421 agcacatcgc gtgccggggt gaataattcc atcgtcatca gtccgatgtt gcgcccgaca
    21481 accagcggtg cccaacggtt ggcaaaaaga tgtaccaggc tgtccgtgac gccgatggtt
    21541 gcttcgcgat cgctctgtcg acgctgctga tagcggcaca atatgccgta atcaccgatg
    21601 tcttctccgc gctcatgcgc ctgagtcagg gtttccgcaa ggctcatgac atctcgcata
    21661 ccgaggttaa acccttgccc ggcaatcggg tgcagagttt gcgccgcatt gcccaccagc
    21721 acggtacgat gggtaatagc tctggcggcg cgggttaacg ccagcggata agcactgcgt
    21781 ttaccggcgt gggtaatttt tccaagtcgc cagccaaagg ccgactggag ttcacggcaa
    21841 aacttctcgt cgctccacga cagcacctct tcgcgccgtt ccagtggatg acaccagacc
    21901 agcgaacagc gtccgtcaga catcggcaac atcgccagcg ggccatgttg cgtgaagcgt
    21961 tcaaaagcgc ggccttcgtg cgcaacggaa gtcgcaacgt tggcaatcac ggccagttgt
    22021 tcgtaaggct cctgctgcca gtcaacgccg cacgcggtgg ctaacgctga atgggtgcca
    22081 tcagctgcta ccagcacgcg gcccgtcagc gtctcgccac tctccagcgt cacttcaacg
    22141 tgactctgag tacgggcaac gttagccacg cgatcagggc aatgcagcgt tacgccaggt
    22201 gctttacgca gcaatgcaaa cagccgttgc ccgacattgt gcaattcgac aacctgtccc
    22261 agcgccgcca gttggtaatc ttctgcggcg agggtgacaa atccagcgtg accacgatcg
    22321 ctgacatgca cggtagtgat ggcggttgcg taatccgcca gagattgcca gacgccgatg
    22381 cgcgccagtt gctgacaggt ccccgcagcc agcgcaatag ctcgtccatc aaagcccgga
    22441 tgagcatgtg actctggcgc ggtcgcttca atcaaatgta ccggcagcgc cccgtgactt
    22501 aaccgggaaa tagccagcgc cagcgtcgcg cccgccatgc cgccaccgac gatgattacg
    22561 ctcattgctt tctcgcagct gccatcaacg cttcgatttc ttccggcttt ttcaccacgc
    22621 tggcggtgag gttttcgttg ccggtttcgg taatcacaat gtcgtcttca atacgaatgc
    22681 cgataccgcg atattgttct ggcacatccg catccggcgc gatatacaga ccaggctcta
    22741 cggtcagtac catccccggt tccagaatgc gcgagcgatc ctgaccataa acgccaacgt
    22801 catggacatc cagtcctaac cagtggctaa ggccatgcat aaagaaagga cgatgggcgt
    22861 tctgagcgat cagttcatca acatcacctt tcaggatgcc gagttttacc aggccgctaa
    22921 ccatgatgcg caccacttca ccagtgactt ccagaatgga agttcccgga cgatacaggc
    22981 gcaggctggt ttcgagagac tccagcacaa tgtcgtagat ttcacgctgg gcctgggtga
    23041 atttgccgtt gaccgggaag gtgcgggtaa tatcgcccgc gtaacctttg tattcacaac
    23101 ccgcgtcaat caacaccagg tcaccgtcgc gcagttcact ctcgttttcg gtgtagtgca
    23161 gaatgcagcc gttttcaccg ctgccgacaa tggtgttata ggacggatag cgcgcaccgt
    23221 ggcggttaaa ttcgtggtga atttcgcctt ccagatggta ctcgaacatt cccggacggc
    23281 atttttccat cgcccgtgta tgtgccatgg cggtgatttc tcccgcgcgg cggagtacgg
    23341 caatctcttc tggcgatttg aacaggcgca tttcatgaac aacaggacgc cagtcgatca
    23401 tcgttgccgg tgcggtgaga ttttgccgcg aacctttacg cagtttttcc agcgcactgt
    23461 tcatgatttc atcagcatat gcatattcgc cctgggcatg gtaaaccaca tccaggccgt
    23521 taagtagttg ataaagttgc tgattgattt cgctgaatgc cagtgcgcgg tcaacgccca
    23581 gtttctctgg cgcggcatcc tggcctaagc gacggccaaa ccagatctcc gccgtcaggt
    23641 cgcgaacgcg gttaaacaga acgctgtggt tatgagtgtc atcgctttta atcagcacca
    23701 gcaccgcttc caattcgtta aagccggtga agtaccagaa gtcactgttc tgacgatagg
    23761 ggtattcgct gtcggcgcta cgtgttactt ctggtgcagc aaaaatcagc gcggcgctgc
    23821 cgggttgcat ttgctccacc agggcctgac ggcgacgctg aaactcttgc cgggatatct
    23881 cactcataac actctcctta cgttttttgt ttttagtgta gagtcggttt ttgtacttct
    23941 ggcgcggtcg gttgcggatg agtaaaggtg tcgtggcata acagcgcggc aacacgaacg
    24001 tattcgatga tctcttcaag cgacatttca agctcttcct gatcttcgtc ttcgtcgtaa
    24061 cccagttgcg caatgttacg cagatcgtcg atagcttcac cggtttcgcc ggtcacttta
    24121 tccagcttcg gttgcgtaac gccaagacca agcaggaagt gattgaccca acctgccaat
    24181 gcatcagccc gatcgaaaac gctgacatca tcgccatcag gcagataaag ctgaaaaagg
    24241 aagccgtcat cctgcagggc atcgctggtg gcagagtgca ttttacgcag tgcctgtgcc
    24301 agctcatgac cgaaagccat gccttcgttc gtcaggtcgt gaagtagcgg cagccatgag
    24361 ctgtcatcgt taccgccaca tatcatccca ctgattaaac catgcatctc ggcaggggtc
    24421 agccccgtcc cttgttggtt cagatactgg ttcatttcgt tgtaaccagg catttcgttc
    24481 tgtatagaca taagcattcg tcgtcaaagg gaggaatatt catgatatgc taccactttg
    24541 ggccctggtg gaccagaaaa gggcttgtct cttctcatca gggtagctat agtgtcgccc
    24601 cttcgcagac catgggtcta aagacgaagg cagcgcagtc aatcagcagg aaggtggcat
    24661 gtctgcacaa cccgtcgata tccaaatttt tggccgttca ctgcgtgtga actgcccgcc
    24721 tgaccaaagg gatgcgttga atcaggcagc ggacgatctg aaccaacggt tgcaagatct
    24781 gaaagaacgc actagagtca caaatactga acagttggtc ttcattgccg cattgaatat
    24841 cagctatgag ttagcgcaag aaaaagcgaa gactcgtgac tacgcggcaa gtatggaaca
    24901 gcgtattcgg atgctgcagc agaccataga acaagcgtta cttgaacaag gtcgcatcac
    24961 cgaaaaaact aaccaaaact ttgaatgaca cttttcggtt tactgtggta gagtaaccgt
    25021 gaagacaaaa tttctctgag atgttcgcaa gcgggccagt cccctgagcc gatatttcat
    25081 accacaagaa tgtggcgctc cgcggttggt gagcatgctc ggtccgtccg agaagcctta
    25141 aaactgcgac gacacattca ccttgaacca agggttcaag ggttacagcc tgcggcggca
    25201 tctcggagat tcccttctta tctggcacca gccatgacgc aactaccaga actcccactg
    25261 acattatccc gacaagaaat ccgcaaaatg attcggcaac gtcgtcgtgc gttaacgccg
    25321 gaacaacagc aggaaatggg tcaacaagcc gctacccgga tgatgactta tcccccggtg
    25381 gtgatggcgc atacggtcgc tgtattcctc tcttttgatg gtgaactcga cacccagccg
    25441 ctaatagaac aactctggcg cgccggtaag cgcgtatatc ttccagtttt gcatcccttt
    25501 agtgccggta atttgctgtt cctgaattac catccgcaaa gcgaactggt gatgaacagg
    25561 ttgaagatcc atgagccaaa actggatgtg cgtgacgtgc tacccctttc ccgattagac
    25621 gtgctgatca caccgctggt cgcctttgat gagtacggtc agcgcctggg aatgggcggt
    25681 ggtttttatg atcggacctt acaaaactgg cagcactata aaacgcaacc ggtgggttat
    25741 gcgcatgatt gtcagttggt ggaaaaactc cccgttgaag agtgggatat ccctcttcct
    25801 gcggtggtta caccgtcgaa agtctgggag tggtaagggc gatacacctg catcgccctg
    25861 attgacatcg ttgattcttt aacctaattt agtgagtaag ggtaagggag gattgctcct
    25921 cccctgtgac tgactgttaa taagctggga aacttatgag taacagtaca atcagtatga
    25981 tggcaagtcg catcataacc cttctccttc aagccctcgc ttcggtgagg gctttaccgt
    26041 tacagcccca tgctgcccag ctatcgtaag ctctctttaa ataaccacaa cgcattgatc
    26101 tacctttaaa tcttttcttc gagcgtgctc gaaaaaccca tgcaaaacag agataatttt
    26161 tgggaaaaag gatctattac ggcagtaaac gtgggcgatg aagtggctat caaaaagttc
    26221 ggttcagggc gatgcacccc gcatcgccct gattgacatc gttgattctt tgacctaatt
    26281 tagtgagtaa gggtaagaga ggatgactcc tcccctgaga ctgactgtta ataagctccg
    26341 aaacttatca gtaacagtac aatcagtatg atgacaagtc gcatcataac ccttctcctt
    26401 taagccctcg cttcggtgag ggctttaccg ttacagcccc atgctgtccc gccatcgtaa
    26461 atccccatta aataaacaca acgcattgat ctgagtttga tttattttct ggagcagact
    26521 cgcaaagtag aatgcgcaac gcggcaacgg tgtggagaag ggataaaaaa acgggcaagt
    26581 cagtgacctg cccgttaatt ttcagagaag gggaattagt acagcagacg ggcgcgaatg
    26641 gtacccggaa tagctttcat tgcctgcagc gctttttcgg caacgtcttc gtcagcttca
    26701 atatcaataa ctacataacc catctgggcg gaagtttgta gatattgcgc ggcgatgttg
    26761 acgccctgct cggcgaagat tttgttcagc gcagttagca cgcccggacg gttttcgtgg
    26821 atgtgcatca gacgacgacc accatgcagt ggcagcgaga cttccgggaa gttcaccgca
    26881 gagagcgttg agccattgtc agaatacttg atcaatttac ccgcaacttc caggccgata
    26941 ttctcctgcg cttcctgagt cgaaccgcca atgtgtggcg tcagaaggac gttgtcgaat
    27001 tcacacagtg gagaggtaaa tggatcgcta ttggtcgccg gttccgtcgg gaatacgtcg
    27061 attgccgccc ccgccagatg tttgctcgcc agcgcatcac acagcgccgg aatatccacc
    27121 acagtaccgc gcgaggcatt aatcagcagc gagccgggct tcattagcga aatctctttc
    27181 gcgcccatca tatttttggt ggacggattc tctggtacat gcagactcac cacatcgctc
    27241 atattcagca ggtcagaaag atgctgtacc tgagtggcgt tgcccagcgg cagtttgttt
    27301 tcaatatcat aaaagtaaac atacattccc agcgattcag ccagaatgcc caattgcgta
    27361 ccaatatgac cgtagccgat gatacccagc tttttgccgc gcgcttcaaa agaacccgcc
    27421 gccagtttgt tccacacgcc acggtgcgct ttagcattgg cttccggcac gccgcgcaat
    27481 agcagcagca gttcgccaat caccagctcc gcaacagagc gcgtatttga gaacggtgcg
    27541 ttaaataccg ggatcccgcg ctttgccgcc gcatccagat caacctggtt tgttccgata
    27601 cagaaacagc caatagcgac cagtttttct gcggcgttga tcacgtcttc agtcagatgg
    27661 gtacgggatc gcaggccgat gaagtgggca tcgcggatgg attcttttaa ttgttcatca
    27721 tccagcgcgc ctttgtgaaa ttcgatgttg gtgtaaccag ctgcacgaag gctttccagc
    27781 gccttttggt gcacgccttc taccagcaga aacttaatct tgtctttctc cagcgatacc
    27841 tttgccattt acccaatcct gtcttttgaa atgttgtgtg cggatttgca tccgcctttc
    27901 aacatatcaa aaaataatat tgcggcaata tgaacgtttg cgtcgcgatg ttgaggaaat
    27961 atcacgcaaa ggtatttctt ggttgaatat gctggtttag aggatttatt tagagcaatc
    28021 gacaatagcc aagtaaaaac gtgacacatg tcaccaaatt taatgaagag aattttttta
    28081 acgggggagg ttcccccgtc agatcatttc acaatggttt tgacaccgtc aggtgtgcca
    28141 atcagcgcaa cgtccgcgcc acggttagca aacaagccaa cagtcaccac gccaggaatc
    28201 gcatttatgg cgttttccat cgctatcggg tcaaggattt ccatgccgtg gacgtcgagg
    28261 atcacgttgc cgttatcggt caccacgccc tgacggtatt ccggacgacc gcccagtttc
    28321 accagctggc gcgccactgc actacgtgcc atcgggataa cttctactgg cagcgggaat
    28381 ttgcccagaa tatcaacctg cttggaagcg tcagcaatac agataaattt ttccgcaacc
    28441 gaagcaatga ttttttcacg ggtcagcgcc gcgccgccgc ctttgatcat ttgcatgtgg
    28501 ccgttgattt catctgcgcc atcaacgtag atgccaaggc tgtcgacttc gttgagatca
    28561 aaaacgtgaa tgccgaggct tttcagtttt tcagtggaag catctgaact ggaaacggcc
    28621 ccttcaatct ggcctttcat tgtaccgagc gcgtcaataa agtgtgcggc ggtggaacct
    28681 gtacctacac caacaatggt gccgggctga acatactgaa gtgccgccca tcctactgct
    28741 tttttcaatt catcctgcgt catgatcgtt tcgcctgtgg tatgaaattt cacacgcatt
    28801 atatacaaaa aaagcgattc agaccccgtt ggcaagccgc gtggttaact catccataaa
    28861 atatcgcgca atggcgggca tcccctttcg ccccgcaaat aaagcataca acggtctggg
    28921 tatgccgctc cacggtgcga acaggcgcac cagttccccc cgttcgcaag cccctgttta
    28981 caggcaaatt gaggcaataa cgccacccca ttcaacacaa ccagggcacg aactcgctga
    29041 gcgaaatgca ttagcgcaaa tccctgatct tttaagctgc gcgtttaacc acggatacaa
    29101 atgttacccg ccgacggctt cggtatatgc aacctgacac aaaattgtgt catagtgcag
    29161 gaaaaagcat ttaccaggag cagacaacag caatgaaacg cccggactac agaacattac
    29221 aggcactgga tgcggtgata cgtgaacgag gatttgagcg cgcggcacaa aagctgtgca
    29281 ttacacaatc agccgtctca cagcgcatta agcaactgga aaatatgttc gggcagccgc
    29341 tgttggtgcg taccgtaccg ccgcgcccga cggaacaagg gcaaaaactg ctggcactgc
    29401 tgcgccaggt ggagttgctg gaagaagagt ggctgggcga tgaacaaacc ggttcgactc
    29461 cgctgctgct ttcactggcg gtcaacgccg acagtctggc gacgtggttg cttccggcac
    29521 tggctcctgt gttggctgat tcgcctatcc gcctcaactt gcaggtagaa gatgaaaccc
    29581 gtactcagga acgtctgcgc cgcggcgaag tggtcggcgc ggtgagtatt caacatcagg
    29641 cgctgccgag ttgtcttgtc gataaacttg gtgcgctcga ctatctgttc gtcagctcaa
    29701 aaccctttgc cgaaaaatat ttccctaacg gcgtaacgcg ttcggcatta ctgaaagcac
    29761 ctgtggtcgc ctttgaccat cttgacgata tgcaccaggc ctttttgcag caaaacttcg
    29821 atctgcctcc aggcagcgtg ccctgccata tcgttaattc ttcagaagcg ttcgtgcaac
    29881 tt
//
LOCUS       DAJTZL010000034        29548 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.034, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000034 DAJTZL010000000
VERSION     DAJTZL010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29548
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.034"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..689
                     /gene="dgt"
                     /locus_tag="OO544_001334"
     CDS             <1..689
                     /gene="dgt"
                     /locus_tag="OO544_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dNTP triphosphohydrolase"
                     /protein_id="HCR6766970.1"
                     /translation="EDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRS
                     NSLSRSTEDQFFMYLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLL
                     KLYKNVAVKHVFSHPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRF
                     PIESRLFHKLSTRHRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWD
                     EYRRLMAVEQ"
     gene            819..2243
                     /gene="degP"
                     /locus_tag="OO544_001335"
     CDS             819..2243
                     /gene="degP"
                     /locus_tag="OO544_001335"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HCR6766971.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            2398..3555
                     /gene="cdaR"
                     /locus_tag="OO544_001336"
     CDS             2398..3555
                     /gene="cdaR"
                     /locus_tag="OO544_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
                     /protein_id="HCR6766972.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDAEDHRKRVEQLITRMKEYGQLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIHRNTLEYRLNRISELTGLDLGNFDDRLLLYVALQLDEER"
     gene            complement(3644..4030)
                     /locus_tag="OO544_001337"
     CDS             complement(3644..4030)
                     /locus_tag="OO544_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HCR6766973.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(4192..5004)
                     /gene="yaeI"
                     /locus_tag="OO544_001338"
     CDS             complement(4192..5004)
                     /gene="yaeI"
                     /locus_tag="OO544_001338"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase YaeI"
                     /protein_id="HCR6766974.1"
                     /translation="MISRRRLLQATAATIATSSGFGYMHYWELGWFELIRHRLAFFKD
                     NAAPFKILFLADLHYSRFVPLSLISDAIALGIEQKPDLILLGGDYVLFDMPLNFSAFS
                     DVLSPLAECAPTFACFGNHDRPVGTEKNHLIGETLKSVGITVLFNQATVIATPNRQFE
                     LVGTGDLWAGQCKPPPASEANLPRLMLAHNPDSKEVMRDEPWDLMLCGHTHGGQLRVP
                     LVGEPFAPVEDKRYVAGLNAFGERHIYTTRGVGSLYGLRLNCRPEVTMLELV"
     gene            complement(5059..5883)
                     /gene="dapD"
                     /locus_tag="OO544_001339"
     CDS             complement(5059..5883)
                     /gene="dapD"
                     /locus_tag="OO544_001339"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HCR6766975.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(5914..8586)
                     /gene="glnD"
                     /locus_tag="OO544_001340"
     CDS             complement(5914..8586)
                     /gene="glnD"
                     /locus_tag="OO544_001340"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HCR6766976.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCVWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLHYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(8648..9442)
                     /gene="map"
                     /locus_tag="OO544_001341"
     CDS             complement(8648..9442)
                     /gene="map"
                     /locus_tag="OO544_001341"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HCR6766977.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFIEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            9810..10535
                     /gene="rpsB"
                     /locus_tag="OO544_001342"
     CDS             9810..10535
                     /gene="rpsB"
                     /locus_tag="OO544_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HCR6766978.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFEKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            10793..11644
                     /gene="tsf"
                     /locus_tag="OO544_001343"
     CDS             10793..11644
                     /gene="tsf"
                     /locus_tag="OO544_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HCR6766979.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            11791..12516
                     /gene="pyrH"
                     /locus_tag="OO544_001344"
     CDS             11791..12516
                     /gene="pyrH"
                     /locus_tag="OO544_001344"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HCR6766980.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            12808..13365
                     /gene="frr"
                     /locus_tag="OO544_001345"
     CDS             12808..13365
                     /gene="frr"
                     /locus_tag="OO544_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HCR6766981.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            13457..14653
                     /gene="ispC"
                     /locus_tag="OO544_001346"
     CDS             13457..14653
                     /gene="ispC"
                     /locus_tag="OO544_001346"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HCR6766982.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            14842..15600
                     /gene="ispU"
                     /locus_tag="OO544_001347"
                     /gene_synonym="uppS"
     CDS             14842..15600
                     /gene="ispU"
                     /locus_tag="OO544_001347"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HCR6766983.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGRIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            15613..16470
                     /gene="cdsA"
                     /locus_tag="OO544_001348"
     CDS             15613..16470
                     /gene="cdsA"
                     /locus_tag="OO544_001348"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCR6766984.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            16482..17834
                     /gene="rseP"
                     /locus_tag="OO544_001349"
     CDS             16482..17834
                     /gene="rseP"
                     /locus_tag="OO544_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HCR6766985.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVMALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            17864..20296
                     /gene="bamA"
                     /locus_tag="OO544_001350"
     CDS             17864..20296
                     /gene="bamA"
                     /locus_tag="OO544_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HCR6766986.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            20418..20903
                     /gene="skp"
                     /locus_tag="OO544_001351"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             20418..20903
                     /gene="skp"
                     /locus_tag="OO544_001351"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HCR6766987.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            20907..21932
                     /gene="lpxD"
                     /locus_tag="OO544_001352"
     CDS             20907..21932
                     /gene="lpxD"
                     /locus_tag="OO544_001352"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HCR6766988.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            22037..22492
                     /gene="fabZ"
                     /locus_tag="OO544_001353"
     CDS             22037..22492
                     /gene="fabZ"
                     /locus_tag="OO544_001353"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HCR6766989.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            22496..23284
                     /gene="lpxA"
                     /locus_tag="OO544_001354"
     CDS             22496..23284
                     /gene="lpxA"
                     /locus_tag="OO544_001354"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HCR6766990.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            23284..24432
                     /gene="lpxB"
                     /locus_tag="OO544_001355"
     CDS             23284..24432
                     /gene="lpxB"
                     /locus_tag="OO544_001355"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HCR6766991.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKEHVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNAARDVLGIPHDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            24429..25025
                     /gene="rnhB"
                     /locus_tag="OO544_001356"
     CDS             24429..25025
                     /gene="rnhB"
                     /locus_tag="OO544_001356"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HCR6766992.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEHGATEHHRRSFGPVKRALGLAS"
     gene            25062..28544
                     /gene="dnaE"
                     /locus_tag="OO544_001357"
     CDS             25062..28544
                     /gene="dnaE"
                     /locus_tag="OO544_001357"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HCR6766993.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERVKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEAHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            28557..29516
                     /gene="accA"
                     /locus_tag="OO544_001358"
     CDS             28557..29516
                     /gene="accA"
                     /locus_tag="OO544_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HCR6766994.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAVSLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
BASE COUNT         7159 a         7120 c         8160 g         7109 t
ORIGIN      
        1 ttgaagatgc ggtagagaaa agaatattta ccgttgagca gctttatcat catttgcacg
       61 aggcgtgggg tcagcatgag aaaggttcgc tcttttcgct agtggttgaa aatgcctggg
      121 aaaaatcacg ctcaaatagt ttaagccgca gtacggaaga tcagtttttt atgtatttac
      181 gggtaaacac cctaaataaa ctggtaccct acgcggcaca acgatttatt gataatctgc
      241 ctgcgatttt cgccggaacg tttaatcatg cattattgga agatgccagc gaatgcagcg
      301 atcttcttaa gctatataaa aatgtcgctg taaaacatgt gtttagccat ccggatgtcg
      361 agcagcttga attgcagggc tatcgggtca ttagcggatt attagagatt tatcgtcctt
      421 tattaagcct gtcgttatca gactttactg aactggtaga aaaagaacgg gtgaaacgtt
      481 tccctattga gtcgcgctta ttccacaaac tctcgacgcg ccatcggctg gcctatgtcg
      541 aggctgtcag taaattaccg tcagattctc ctgagtttcc gctatgggaa tattattacc
      601 gttgccgcct actgcaggat tatatcagcg gtatgaccga cctctatgcg tgggatgaat
      661 accgacgtct gatggccgta gaacaataac caggcttttg taaagacgaa caataaattt
      721 ttaccttttg cagaaacttt agttcggaac ttcgcgctat aaaacgaatc tgaagaacac
      781 agcaattttg cgttatctgt taatcgagac tgaaatacat gaaaaaaacc acattagcac
      841 tgagtgcact ggctctgagt ttaggtttgg cgttatctcc gctctctgca acggcggctg
      901 agacttcttc agcaacgaca gcccagcaga tgccaagcct tgcaccgatg ctcgaaaagg
      961 tgatgccttc agtggtcagc attaacgtag aaggtagcac aaccgttaat acgccgcgta
     1021 tgccgcgtaa tttccagcag ttcttcggtg atgattctcc gttctgccag gaaggttcac
     1081 cgtttcagag ctctccgttc tgccaggggg gccagggcgg taatggcggc ggccagcaac
     1141 agaaattcat ggcgctgggt tccggcgtca tcattgatgc cgataaaggc tatgtcgtca
     1201 ccaacaacca tgttgttgat aacgcgacgg tgattaaagt ccagctgagc gatggtcgta
     1261 agttcgacgc gaagatggtt ggcaaagatc cgcgctctga tatcgcgctg atccaaatcc
     1321 agaacccgaa aaacctgacc gcaattaaga tggcggattc tgatgcactg cgcgtgggtg
     1381 attacaccgt agcgattggt aacccgtttg gtctgggcga gacggtaact tccgggattg
     1441 tctctgcgct ggggcgtagc ggcctgaatg ctgaaaacta cgaaaacttc atccagaccg
     1501 atgcagcgat caaccgtggt aactccggtg gtgcgctggt taacctgaac ggcgaactga
     1561 tcggtatcaa caccgcgatc ctcgcaccgg acggcggcaa catcggtatc ggttttgcta
     1621 tcccgagtaa catggtgaaa aacctgacct cgcagatggt ggaatacggc caggtgaaac
     1681 gcggtgagct ggggattatg gggactgagc tgaactccga actggccaaa gcgatgaaag
     1741 ttgacgccca gcgcggtgct ttcgtaagcc aggttctgcc taattcctcc gctgcaaaag
     1801 cgggcattaa agcgggtgat gtgatcacct cactgaacgg taagccgatc agcagctttg
     1861 ccgcactgcg tgctcaggtg ggtactatgc cggtgggcag caaactgacc ctgggcttac
     1921 tgcgcgacgg taagcaggtt aacgtgaacc tggaactgca gcagagcagc cagaatcagg
     1981 ttgattccag ctccatcttc aacggcattg aaggcgctga gatgagcaac aaaggcaaag
     2041 atcagggcgt ggtagtgaac aacgtgaaaa cgggcactcc ggctgcgcag atcggcctga
     2101 agaaaggtga tgtgattatt ggcgcgaacc agcaggcagt gaaaaacatc gctgaactgc
     2161 gtaaagtgct cgacagcaaa ccgtctgtgc tggcactgaa cattcagcgc ggcgacagca
     2221 ccatctacct gttaatgcag taatctccct caaccccttc ctgaatacgg gaaggggttc
     2281 tccttacaat ctgtgaactt cgccacaact ccatacatct tcatcatcct ttgggcattt
     2341 gcacaatgcc gtacgttacg tacttcctta tgctaagccg tgcataacgg aggacttatg
     2401 gctggctggc atcttgatac caaaatggcg caggatatcg tggcacgtac catgcgcatc
     2461 atcgatacca atatcaacgt aatggatgcc cgcgggcgaa ttatcggcag cggcgatcgt
     2521 gagcgtattg gtgaattgca cgaaggtgca ttgctggtac tttcacaggg acgagtcgtc
     2581 gatatcgatg acgcggtagc acgtcatctg cacggtgtgc ggcaggggat taatctaccg
     2641 ttacggctgg aaggtgaaat tgtcggcgta attggcctga caggtgaacc agagaatctg
     2701 cgtaaatatg gcgaactggt ctgcatgacg gctgaaatga tgctggaaca gtcgcggttg
     2761 atgcacctgt tggcgcagga tagccgtttg cgggaagaac tggtgatgaa cctgattcag
     2821 gctgaggaga atactcccgc acttactgaa tgggcgcaac ggctggggat tgatctcaat
     2881 caaccgcgag tggtggctat tgttgaggtc gacagcggtc agcttggcgt ggacagcgca
     2941 atggcggagt tacaacaact gcaaaatgcg ctgactacgc ccgagcgtaa taatctggtg
     3001 gcaattgttt cgctgaccga aatggtggtg ttgaaaccgg cgttgaactc ttttgggcgc
     3061 tgggatgcag aagatcatcg taagcgagtt gaacaactga ttacccgcat gaaagagtac
     3121 ggccagctgc gttttcgcgt ttcactgggc aactatttta ccggtcctgg cagtattgcc
     3181 cgatcctatc gtacggcgaa aacgacgatg gtggtgggta aacagcggat gccagaaagt
     3241 cgctgctatt tttatcagga tctgatgtta cctgtgttac tcgacagttt gcgtggcgac
     3301 tggcaggcca acgaactggc gcgaccgctg gcgcggctga aagcgatgga caataatggc
     3361 ttgctgcgac gaacgctggc ggcgtggttt cgccacaatg tgcaaccgct ggctacgtca
     3421 aaggcgctgt ttattcatcg taatactctg gagtatcggc ttaatcgtat atcggaactg
     3481 accgggcttg atttgggtaa ttttgatgac aggttgctgc tgtatgtggc gttacaactg
     3541 gatgaagagc ggtaggttat gcgttaaggt cgaggcggcg ctggctcatt cccctcagcc
     3601 cgaagggtag aggggaatgt ccgagttaac cgtcagttcg gcattattta cgcgttagtt
     3661 tttccagatc ggcttcaatc tcgctgatct tattggttac gactgactcc aggtgacgta
     3721 agtcgtcgag gatcttacgc ttaagatcaa cttcgctgcg gtcacgctgg cagatttgat
     3781 caagctcatc aatgatatac cgtagattcg ggctgatttc ctggacttct ttataaccct
     3841 gacccacacc atcagcgacg accgttttac gctgacgcgg atatttaaac ttcacgctct
     3901 tggcgaaaaa ctcgcctttg tctttctgga aatagatttt cagaatatcg ttgttggctt
     3961 cctgccggag gctgtaacga tcaatttctt caggattggt aatacccaga cttttcagat
     4021 tgtcgtacat agcgttacct caaaatgagt cagtaaattg tgcttatttt agcatttggc
     4081 atggcccgcc cggctcgact gtgatcgcag aaagcctttt tcttccagac ttctatcatg
     4141 gcgtaaaaat caaaaaatta cctgctttat tgtggtgata aaattcacgt tctacaccag
     4201 ttccagcatc gttacttccg ggcggcagtt cagacgcaaa ccatacaaac tgcccacgcc
     4261 acgggttgtg tagatgtgtc gttcgccaaa ggcatttaat ccggcgacgt aacgtttatc
     4321 ttcgacaggg gcaaaaggtt caccaaccaa cggtacgcgc agttgcccgc cgtgagtatg
     4381 gccgcacagc atcagatccc agggttcatc acgcatgact tctttgctgt cgggattatg
     4441 tgccagcatc agtcttggca gatttgcttc gctggcggga ggtggtttgc attgtccggc
     4501 ccataagtca ccggtgccta ccagttcgaa ttgcctgttc ggcgtggcga tcaccgtagc
     4561 ctggttaaac aacaccgtga tgcccactga tttcaacgtc tcgccaatta agtgattttt
     4621 ttctgtacca acagggcgat cgtggttgcc gaagcaggca aacgtcggcg cacattcggc
     4681 aaggggggag aggacatcac taaacgccga aaaattcagc ggcatatcaa ataatacgta
     4741 atcgccgccc agtaatatca aatcgggctt ttgttctatg ccaagagcaa tcgcgtcaga
     4801 aatcaggctt aaaggaacaa aacgagagta atggagatcg gccagaaaaa gaattttgaa
     4861 tggtgctgcg ttgtctttaa aaaaggcgag gcggtgacgg attaattcaa accagcctag
     4921 ctcccagtaa tgcatataac cgaaacctga gctcgtggct atcgtcgcgg cagtagcctg
     4981 caacaatcgg cggcgtgaaa tcatcgctca tccctgcaaa aaaaatcggg cagcgtcgtg
     5041 ctgcccgtgt gcatactttt agtcgatggt acgcagcagt tcgttaatgc cgactttgcc
     5101 gcgagttttc gcgtcaactt tcttaacgat aaccgcacag tagaggctgt atttgccatc
     5161 ttttgacggc agattacctg aaacaaccac agaccccgcc ggaacgcgac cgtagtggat
     5221 ttcgccggtt tcacggtcgt aaatacgggt gctctgacca atgtatacgc ccatggaaat
     5281 gacggaacct tcttcgacaa tcaccccttc aaccacttca gagcgtgcgc caatgaagca
     5341 gttatcttca atgatggttg ggttagcctg cagcggttcc agcacgccgc cgatgcccac
     5401 gccaccggaa aggtggacgt ttttaccaat ctgcgcacaa gaaccgacgg tcgcccaggt
     5461 atcaaccatg gtgccttcat caacatatgc gccgatgttg acgtaagacg gcatcagcac
     5521 ggtgttacgg gcaataaacg caccctgacg taccgccgct ggtggcacaa cgcggaagcc
     5581 ttctttctgg aaacgtgctt cgtcgtagtc ggcgaatttc atcggcactt tgtcgaagta
     5641 gcggctttct gccccttcga tcacctgatt atcattaata cggaaagaga gcagcaccgc
     5701 ttttttcaac cactgatgcg tcacccactg accgtcaatt ttttccgcta cacgcagtgc
     5761 accggaatcc agcagggcga tcacctgatt taccgcttcg cgggtaacgg tgtctgcgtt
     5821 ggctggcgtg atctcggcac ggcgttcaaa agcggtttca ataatgttct gtaactgctg
     5881 cattgttaaa ctcttttcat atcagtaaac acatcaccct ttatcgtttg gattgagggc
     5941 ctctgtcaac cgctgatgca cttcctgctg caactcgtta ttaagtgcac gccggtcagc
     6001 ggtggcaatt atgaataaat cttctactcg ctcgccaatg gttgtaattc gggcaccatg
     6061 aagcgaaatt cccagatcgg caaaaatttt cccgactctc gccagcagtc ctggctggtc
     6121 gagcgcgata agctcaagga acgatttgcg gtcggtatgg gttggcaaaa acgtcacttc
     6181 ggtttcaaca gtaaagtgac gtagtttagc cggttgacga cgcggctgtg gtggctgcca
     6241 gctactttgt gtcagtacct gctccagacc aaaccgaata acctcatgac gatctgcgga
     6301 tagcgggcta ccatcgggtt ccagcacgat gaaggtatcc atcgccatac cgtcgcgagt
     6361 ggtgaaaatt tgtgcgtcgt gaacacttaa attgcggcgg tctaattcgg cacagacggc
     6421 ggcaaacaga taggggcggt ccgggctcca gataaaaatc tcggtgcctc cacgcgtagc
     6481 ctgcgggcta agcaatacca gcggtttgct taaatcatgc tgtaataaat ggcgggcatg
     6541 ccaggccagt tgatttgggc tatggcggac aaaatagtta gcacgacagc gtgaccaaat
     6601 ttggtgcagc gcctcttcgt cgatgttatc catgcgcagt agtgccagtg cctggagttg
     6661 gtgatggcga acccgttcgc gcatatccgg cgtgttttgc attccgcgtc gtagctgctt
     6721 ttcggtggca aaatagagtt cgcgcaacag actttgcttc cagctattcc acagcgtttc
     6781 gttggtggcg caaatgtcag ccactgtcag gcataccaga tagtgcagac gattttccgt
     6841 ttgaacttct tcggcaaact gcttgatgac ttcagggtcc tgaatatcgc ggcgttgtgc
     6901 tgtcaccgac atcagcaagt gctggcgaac cagccaggca accagctgtg tttcgcgtga
     6961 gttcagcccg tggagttctg caaaatgcac tacatcctga gcaccgagaa tggagtggtc
     7021 gccgccgcgt cctttggcga tatcgtgaaa cagcgcggcg atgaaaatca gctcagttga
     7081 cggcaggcgc ggccagacgt ccacacacaa cggatggcgc tggcgcgttt cttcactggc
     7141 aaaactctcc agtttcagca tcacgcggat agtatgttca tccaccgtgt aggcgtggaa
     7201 cagatcaaac tgcatctgcc cgacgatatg cgaccattgc ggcatatacg cgccgagcac
     7261 gctatggcga tgcattggca atagcccgcg ccgcaccgct ccggggtgac gcagaatgct
     7321 caaaaacagt tttcgtgctt ccggaatatt acacagcggt tgttgcagat ggcgacgggc
     7381 atggcgtaac tggcgcagcg tggtggagta aatgccggtg atcgcactgt tgcgcaccat
     7441 ggtgtagaac atacgcaaga tggcttccgg ctggcgcata aatagtgttt catcacgcag
     7501 gtcgattagc gtaccgcgta gctgaaactc atcgtcgatt ggacgtggtt tttcgtcggc
     7561 gggaagggcg aggatggctt catcgaacag ttgcagcagc atctggttga gttcactgac
     7621 gcggcgtgta acgcggaagt aatccttcat catccgctcg accggttcgt tgccttcacc
     7681 actgtaattc agacgctgag cgacgctaag ttggcgatcg aataacaggc gattatcgta
     7741 acggctgacg accagatgca gggcaaagcg aatacgccac agtatatgca gacattcgtt
     7801 taattccgcc cgctccgctg aggttaagaa gccaaaccca accatttcat ccagcgatgt
     7861 tgcgccaaaa tgacggcggg ccacccattg cagagtgtgg atatcgcgca agccgccagg
     7921 gctacttttg atgtctggtt caagattgta gctggtgccg tggtaacgct gatggcgctg
     7981 gttctgttct tcaactttcg ccgcgtagaa cttgtcggaa ggccagaatc cttcgctaaa
     8041 aatatgtttt tgcagttcga ggaagagcgc aacatcgcca attaataagc gggattcgat
     8101 taaattggtg gcgacggtta aatccgataa cccttccagc atgcactctt caagcgtgcg
     8161 cacactatga ccgacttcca gctttacatc ccagagcagc gttaacagct cgcccacttt
     8221 ttgcgcctga tcgtccggga gctttttacg gcttaaaatc agtaaatcga tgtcagaaag
     8281 tggatgcagc tcgccacgac cgtagccacc gacggcgacc aatgccaggt cggcaatctg
     8341 gctgaatccc gcttcaatcc ataatcgttg caggagctgg tcgataaact cggtgcgcgc
     8401 ctcaatcaac tgttctgcag agatcccatt gtcaaaggca tcacccagcc aacgctggaa
     8461 agtatcgata tgggctttta tcccaccgac ggttaattca tcacggggcc agacgcatgg
     8521 attttgcggt tgaccgggca gggtggggag agcggtgttt gcgtactgtt ctggaagggt
     8581 attcattgtg cgccacccat aagattaaat tatcgcatta aaaaagccgg cattcgccgg
     8641 cttcatctta ttcgtcgtgc gagattatcg ccgggatggt gtcatccttg cgtagcgtca
     8701 gaatttcgca gccgttatca gtcaccacaa tagtatgctc atattgtgca gacaagctgc
     8761 gatctttggt ttttaccgtc cagccatctt tcatggtgcg gatctctttt ttacccgcgt
     8821 tgaccattgg ctcgatggtg aacgtcatcc caggtttcag tacgacgttg gtttcacggg
     8881 agtcatagtg cagcacctgc ggttcttcat ggaagccgcg accaataccg tgtccgcaat
     8941 attcacgaac gacggagaag ccttctgctt cgataaactt ctgaatcgcc gcaccgattt
     9001 cgcgcaggtt aatgcctggt ttgaccatgc gcagcgccag gtacaggctt tcttgcgtga
     9061 tgcggcacag acgttcgccc atgatggtcg gcttaccgac gataaacatt ttcgaggtat
     9121 cgccgtggaa accatctttg attacggtga catcaatgtt aacgatatcg ccatctttca
     9181 gcagcttcgc atcgtccggg ataccgtggc acaccacttc attaatagag atgcaaacgg
     9241 atttcggata gccgtgatag ccgaggcagg cagaaaccgc gtgttgttca ttaacaatgt
     9301 aatcattaca gatgcgatcc agctcgccgg tgctgacgcc cggtttaaca tacggttcga
     9361 tcatctccag cacttcggca gccagtcggc cagcgacgcg cattttttcg atatcttctg
     9421 gggtcttgat tgagatagcc attaattctg tccatcagcg tcggtgatac cgacaatata
     9481 tatgtaagtg ccgtcaatgg tatcacaccc gggcaaattg agaatcattc tgaatttcgc
     9541 caaacgtgcc actgaacgtt ttctataata gaaaattcaa cgtctgatgc tgtacacagc
     9601 gccaacaatt attggtgtcc atgacgtatt tgtggtataa agcgcgccgg acttccgatc
     9661 catttcgtat acacagactg gacggaagcg acaatctcac tttgtgtaac aacacacacg
     9721 tatcggcaca tattccgggg tgccctttgg ggtcggtaat atgggatacg tggaggcata
     9781 accccaactt ttatatagag gttttaatca tggcaactgt ttccatgcgc gacatgctca
     9841 aggctggtgt tcacttcggt caccagaccc gttactggaa cccgaaaatg aagccgttca
     9901 tcttcggtgc gcgtaacaaa gttcacatca tcaaccttga gaaaactgta ccgatgttca
     9961 acgaagctct ggctgaactg aacaagattg cttctcgcaa aggtaaaatc cttttcgttg
    10021 gtactaaacg cgctgcaagc gaagcggtga aagacgctgc tctgagctgc gaccagttct
    10081 tcgtgaacca tcgctggctg ggcggtatgc tgactaactg gaaaaccgtt cgtcagtcca
    10141 tcaaacgtct gaaagacctg gaaactcagt ctcaggacgg tactttcgaa aagctgacca
    10201 agaaagaagc gctgatgcgc actcgtgagc tggagaaact ggaaaacagc ctgggcggta
    10261 tcaaagacat gggcggtctg ccggacgctc tgtttgtaat cgatgctgac cacgaacaca
    10321 ttgctatcaa agaagcaaac aacctgggta ttccggtatt tgctatcgtt gataccaact
    10381 ctgatccgga cggtgttgac ttcgttatcc cgggtaacga cgacgcaatc cgtgctgtga
    10441 ccctgtacct gggcgctgtt gctgcaaccg tacgtgaagg ccgttctcag gatctggctt
    10501 cccaggcgga agaaagcttc gtagaagctg agtaataagg cttgataact cccccaaaat
    10561 agttcgagtt gcagaaaggc ggcaagctcg agaattcccg ggagcttaca tcagtaagtg
    10621 accgggatga gcgagcgaag acaacgcatc tgcggcgcga aatatgaagg gggagagccc
    10681 ttatagacca ggtagtacac gtttggttag ggggcctgca tatggccccc tttttcactt
    10741 ttatatctgt gcggtttaat gccgggcaga tcacatctcc gaggatttta gaatggctga
    10801 aattaccgca tccctggtaa aagagctgcg tgagcgtact ggcgcaggca tgatggattg
    10861 caaaaaagca ctgactgaag ctaacggcga catcgagctg gcaatcgaaa acatgcgtaa
    10921 gtccggtgct attaaagcag cgaaaaaagc aggcaacgtt gctgctgacg gcgtgatcaa
    10981 aaccaaaatc gacggcaact acggcatcat tctggaagtt aactgccaga ctgacttcgt
    11041 tgcaaaagac gctggtttcc aggcgttcgc agacaaagtt ctggacgcag ctgttgctgg
    11101 caaaatcact gacgttgaag ttctgaaagc acagttcgaa gaagaacgtg ttgcgctggt
    11161 agcgaaaatt ggtgagaaca tcaacattcg ccgcgttgct gcgctggaag gcgacgttct
    11221 gggttcttat cagcacggtg cgcgtatcgg cgttctggtt gctgctaaag gcgctgacga
    11281 agagctggtt aaacacatcg ctatgcacgt tgctgcaagc aagccagaat tcatcaaacc
    11341 agaagacgta tccgctgaag tggtagaaaa agaataccag gtacagctgg atatcgcgat
    11401 gcagtctggt aagccgaaag aaatcgcaga gaaaatggtt gaaggccgca tgaagaaatt
    11461 caccggcgaa gtttctctga ccggtcagcc gttcgttatg gaaccaagca aaactgttgg
    11521 tcagctgctg aaagagcata acgctgaagt gactggcttc atccgcttcg aagtggggga
    11581 aggcatcgag aaagttgaga ctgactttgc agcagaagtt gctgcgatgt ccaagcagtc
    11641 ttaattatcg aaaaggagcc gcctgagggc ggcttctttt tgtgcccatc ttgtaaattc
    11701 agctaaccct tgtggggctg cgctgaaaag cgacgtacaa tgtcgctagt attaattcat
    11761 ttcaatcgtt gacagtctca ggaaagaaac atggctacca atgcaaaacc cgtctataaa
    11821 cgcattctgc ttaagttgag tggcgaagct ctgcagggca ctgaaggctt cggtattgat
    11881 gcaagcatac tggatcgtat ggctcaggaa atcaaagaac tggttgaact gggtattcag
    11941 gttggtgtgg tgattggtgg gggtaacctg ttccgtggcg ctggtctggc gaaagcgggt
    12001 atgaaccgcg ttgtgggcga ccacatgggg atgctggcga ccgtaatgaa cggcctggca
    12061 atgcgtgatg cactgcaccg cgcctatgtg aacgctcgtc tgatgtccgc tattccattg
    12121 aatggcgtgt gcgacagcta cagctgggca gaagctatca gcctgttgcg caacaaccgt
    12181 gtggtgatcc tctccgccgg tacaggtaac ccgttcttta ccaccgactc agcagcttgc
    12241 ctgcgtggta tcgaaattga agccgatgtg gtgctgaaag caaccaaagt tgacggcgtg
    12301 tttaccgctg atccggcgaa agatccaacc gcaaccatgt acgagcaact gacttacagc
    12361 gaagtgctgg aaaaagagct gaaagtcatg gacctggcgg ccttcacgct ggctcgtgac
    12421 cataaattac cgattcgtgt tttcaatatg aacaaaccgg gtgcgctgcg ccgtgtggta
    12481 atgggtgaaa aagaagggac tttaatcacg gaataattcc cgtgatggat aaataagggt
    12541 aagattccgc gtaagtatcg cgggggcgta agtctggtta taaggcgtta ttgttgcagg
    12601 cagtttggtc acggccagcg cgcagcaacc ggagcgtaca aaagtacgtg aggatggcga
    12661 gcactgctcg gggccaaaat ggcaaataaa atagcctaat aatccagacg attacccgta
    12721 atatgtttaa tcagggctat acttagcaca cttccactgt gtgtgactgt ctggtctgac
    12781 tgagacaagt tttcaaggat tcgtaacgtg attagcgata tcagaaaaga tgctgaagta
    12841 cgcatggaca aatgcgtaga agcgttcaaa acccaaatca gcaaaatacg cacgggtcgt
    12901 gcttctccca gcctgctgga tggcattgtc gtggaatatt acggcacgcc gacgccgctg
    12961 cgtcagctgg caagcgtaac ggtagaagat tcccgtactc tgaaaatcaa cgtgtttgat
    13021 cgttcaatgt ctccggccgt tgaaaaagcg attatggcgt ccgatcttgg cctgaacccg
    13081 aactctgcgg gtagcgacat ccgtgttccg ctgccgccgc tgacggaaga acgtcgtaaa
    13141 gatctgacca aaatcgttcg tggtgaagca gaacaagcgc gtgttgcagt acgtaacgtg
    13201 cgtcgtgacg cgaacgacaa agtgaaagca ctgttgaaag ataaagagat cagcgaagac
    13261 gacgatcgcc gttctcagga cgatgtacag aaactgactg atgctgcaat caagaaaatt
    13321 gaagcggcgc tggcagacaa agaagcagaa ctgatgcagt tctgatttct tgaacgacaa
    13381 aaacgccgct cagtagatcc ttgcggatcg gctggcggcg ttttgctttt tattctgtct
    13441 caactctgga tgtttcatga agcaactcac cattctgggc tcgaccggct cgattggttg
    13501 cagcacgctg gacgtggtgc gccataatcc cgaacacttc cgcgtagttg cgctggtggc
    13561 aggcaaaaat gtcactcgca tggtagaaca gtgcctggaa ttctctcccc gctatgccgt
    13621 aatggacgat gaagcgagtg cgaaacttct taaaacgatg ctacagcaac agggtagccg
    13681 caccgaagtc ttaagtgggc aacaagccgc ttgcgatatg gcagcgcttg aggatgttga
    13741 tcaggtgatg gcagccattg ttggcgctgc tgggctgtta cctacgcttg ctgcgatccg
    13801 cgcgggtaaa accattttgc tggccaataa agaatcactg gttacctgcg gacgtctgtt
    13861 tatggacgcc gtaaagcaga gcaaagcgca attgttaccg gtcgatagcg aacataacgc
    13921 catttttcag agtttaccgc aacctatcca gcataatctg ggatacgctg accttgagca
    13981 aaatggcgtg gtgtccattt tacttaccgg gtctggtggc cctttccgtg agacgccatt
    14041 gcgcgatttg gcaacaatga cgcccgatca agcctgccgt catccgaact ggtcgatggg
    14101 acgtaaaatt tctgtcgatt cggctaccat gatgaacaaa ggtctggaat acattgaagc
    14161 gcgttggctg tttaacgcca gcgccagcca gatggaagtg ctgattcacc cgcagtcagt
    14221 gattcactca atggtgcgct atcaggacgg cagtgttctg gcgcagctgg gggaaccgga
    14281 tatgcgtacg ccaattgccc acaccatggc atggccgaat cgcgtgaact ctggcgtgaa
    14341 gccgctcgat ttttgcaaac taagtgcgtt gacatttgcc gcaccggatt atgatcgtta
    14401 tccatgcctg aaactggcga tggaggcgtt cgaacaaggt caggcagcga cgacagcatt
    14461 gaatgccgca aacgaaatca ccgttgctgc ttttcttgcg caacaaatcc gctttacgga
    14521 tatcgctgcg ttgaatttat ctgtactgga aaaaatggat atgcgcgaac cacaatgtgt
    14581 ggacgatgtg ttatctgttg atgcgaacgc gcgtgaagtc gccagaaaag aggtgatgcg
    14641 tctcgcaagc tgaggataat ccggctacag agagtcgcgc tatttgttag cgtagggctt
    14701 cagtgatata gtctgcgcca tctgatcgta agtagttggc tttataaggt caggtatgcc
    14761 gtggttttac acggcttttt tttgtatagg cttcagtatt cctgagtacc gtaaaccctg
    14821 tcagggaata aaaaacgcgt gatgttgtct gctactcaac cacttagcga aaaattgcca
    14881 gcgcatggct gccgtcatgt tgcgatcatt atggacggca atggtcgctg ggcaaaaaag
    14941 caagggagga ttcgtgcctt tgggcataaa gccggggcaa aatccgtccg ccgggctgtc
    15001 tcttttgcgg ccaacaacgg tattgaggcg ttaacgctgt atgcctttag tagtgaaaac
    15061 tggaaccgac cagcgcagga agtcagtgcg ttaatggaac tgtttgtgtg ggcgctcgat
    15121 agcgaagtaa aaagtctgca ccgacataac gtgcgtctgc gtattattgg cgataccagt
    15181 cgctttaact cgcgtttgca agaacgtatt cgtaaatctg aagcgctaac agctgggaat
    15241 accggtctga cgctgaatat tgcggcgaac tacggtggac gttgggatat agtccaggga
    15301 gtcaggcaac tggctgaaaa ggtgcagcaa ggaaacctgc aaccagatca gatagatgaa
    15361 gagatgctaa accagcatgt ctgtatgcat gaactggccc ctgtagattt agtaattagg
    15421 actggggggg agcatcgcat tagtaacttt ttgctttggc aaattgccta tgccgaactt
    15481 tactttacag atgttctctg gcctgatttc gatgaacaag actttgaagg ggcgttaaat
    15541 gcctttgcta atcgagagcg tcgtttcggc ggcaccgagc ccggtgatga aacagcctga
    15601 tggggggcgc ttttgctgaa gtatcgcctg atatctgctt ttgtgttaat acccgtcgtc
    15661 atcgcggcgt tgtttctgtt gccgccggtg gggttcgcca tcgtaacgct ggtggtctgc
    15721 atgctggcag cgtgggaatg gggacagctt agcggtttta ccactcgttc gcagcgagta
    15781 tggttggcgg tgttatgcgg gttattgttg gcgctgatgc tttttctgtt gccggaatat
    15841 caccgaaata ttcatcaacc gctggttgaa atctcacttt gggcttcgtt gggttggtgg
    15901 attgtcgcgc tattgctggt gctgttttac ccaggttccg cagcaatctg gcgtaactct
    15961 aaaacattgc gccttatttt tggcgtgcta accattgttc ccttcttctg gggcatgctg
    16021 gcgttacggg cctggcacta tgacgagaat cattacagtg gcgcaatatg gctgctctat
    16081 gtcatgatcc tggtatgggg cgctgactcc ggcgcatata tgtttggcaa attgtttggt
    16141 aaacataagc tggcaccgaa ggtttctccg ggtaaaacct ggcaaggctt tataggtgga
    16201 ctagcgaccg cagcggtaat ctcatggggt tatggcatgt gggcgaatct cgacgtcgct
    16261 cccgtcacct tactcatttg ctctattgtc gcagcgttag cctcagtgct cggcgatctg
    16321 accgagagta tgtttaagcg tgaagcagga attaaggaca gcggtcattt aattccagga
    16381 cacggtggta ttttagatcg tattgatagc ctgacggctg cggtaccggt ctttgcttgc
    16441 ttgttgttac tggtattcag gacgctttaa cggaaggtaa tatgctgagt tttctctggg
    16501 atttggcttc gttcatcgtt gcactgggtg tacttatcac cgtgcatgaa tttggtcatt
    16561 tctgggttgc ccggcgttgt ggtgttcgcg ttgagcgttt ctcaataggg tttggtaagg
    16621 cgctctggcg gcgaactgat aagctcggca ccgaatatgt tatggccctg attccgctgg
    16681 gcggttatgt caaaatgctg gatgagcgcg cagaaccggt cgttccggaa ctccgccacc
    16741 atgccttcaa taataaatct gttggccaac gagcggcgat tattgccgca ggtccggttg
    16801 caaacttcat ttttgctatc tttgcctact ggctggtttt tattattggt gtgcctggcg
    16861 tacgtccggt ggttggtgaa atagcagcca attcgatagc tgcggaagca caaattgcac
    16921 caggtacgga actaaaagcc gtagatggta tcgaaacgcc tgattgggat gccgtgcgtt
    16981 tgcagttggt cgataaaatt ggcgatgaaa gcaccaccat tacagtagcg ccatttggca
    17041 gcgaccaacg gcgggatgta aagctcgatt tacgtcactg ggcgtttgag cctgataaag
    17101 aagatccggt atcttcgctg gggattcgtc ctcgtgggcc gcaaattgaa cctgtactgg
    17161 aaaatgtgca gccaaactcg gcggcaagca aggcaggttt gcaagcaggc gacaggatcg
    17221 ttaaagtcga tggtcagccc ttaacgcagt gggtgacctt tgtgatgctt gtccgggata
    17281 acccgggtaa atccttagcg ttagaaatcg aaaggcaggg gagtcccttg tctttgacat
    17341 taatcccgga gagtaaaccg ggtaatggta aagcgattgg ttttgtcggt attgagccga
    17401 aagtcattcc tttgccagat gagtataaag ttgtacgcca gtatgggccg ttcaacgcca
    17461 tcgtcgaagc cacggacaaa acgtggcagc tgatgaagct gacggtcagt atgctgggaa
    17521 aattgatcac cggtgatgtg aaactgaaca acctcagtgg gccgatctct atcgccaagg
    17581 gggctgggat gacagcggaa ctcggggtag tttattacct gccgtttctt gcgcttatta
    17641 gcgtgaactt agggataatt aacctgtttc cgttgcccgt acttgacggg gggcatctgc
    17701 tgttccttgc gatcgaaaag atcaagggcg gaccggtatc cgagcgggtt caagactttt
    17761 gttatcgcat tggctcgatt ctgctggtgc tgttaatggg gcttgcactt ttcaatgatt
    17821 tctctcggtt atgagagtta gttaggaaga acgcataata acgatggcga tgaaaaagtt
    17881 gctcatagcg tcgctgctgt ttagcagcgc caccgtatac ggtgctgaag ggttcgtagt
    17941 gaaagatatt catttcgaag gccttcagcg tgtcgccgtt ggtgcggcac tcctcagtat
    18001 gccggtgcgc acaggcgaca cggttaatga tgaagatatc agtaatacca ttcgcgctct
    18061 gtttgctacc ggcaactttg aggatgttcg cgtccttcgt gatggtgata cccttctggt
    18121 tcaggtaaaa gaacgtccga ccattgccag cattactttc tccggtaaca aatcggtgaa
    18181 agatgacatg ctgaagcaaa acctcgaggc ttctggtgtg cgtgtgggcg aatccctcga
    18241 tcgcaccacc attgccgata tcgagaaagg tctggaagac ttctactaca gcgtcggtaa
    18301 atatagcgcc agcgtaaaag ctgtcgtgac cccgctgccg cgcaaccgtg ttgacctaaa
    18361 actggtgttc caggaaggtg tgtcagctga aatccagcaa attaacattg ttggtaacca
    18421 tgctttcacc accgacgaac tgatctctca tttccaactg cgtgacgaag tgccgtggtg
    18481 gaacgtggta ggcgatcgta aataccagaa acagaaactg gcgggcgacc ttgaaaccct
    18541 gcgcagctac tatctggatc gcggttatgc ccgtttcaac atcgactcta cccaggtcag
    18601 tctgacgcca gataaaaaag gtatttacgt cacggtgaac attaccgaag gcgatcagta
    18661 caagctttct ggcgttgaag tgagcggcaa ccttgccggg cactccgctg aaattgagca
    18721 gctgactaag atcgagccgg gcgagctgta taacggcacc aaagtgacca agatggaaga
    18781 tgacatcaaa aagcttctcg gtcgctatgg ttatgcctat ccgcgcgtac agtcgatgcc
    18841 tgaaattaac gatgccgaca aaaccgttaa attacgcgtg aacgttgatg cgggtaaccg
    18901 tttctacgtg cgtaagatcc gttttgaagg taacgatacc tcgaaagatg ccgtcctgcg
    18961 tcgcgaaatg cgtcagatgg aaggtgcatg gctgggaagc gatctggtcg atcagggtaa
    19021 ggagcgtctg aatcgtctgg gcttctttga aactgtcgat accgataccc aacgtgttcc
    19081 gggtagcccg gaccaggtag atgtagtcta caaggtgaaa gaacgtaaca ccggtagctt
    19141 caacttcggg attggttacg gtactgaaag tggcgtgagc ttccaggctg gcgtgcaaca
    19201 ggataactgg ttaggtacag gttatgctgt tggtatcaac gggaccaaaa acgattacca
    19261 gacctatgct gaattgtcgg taaccaaccc gtacttcact gtagatggtg taagcctcgg
    19321 tggtcgtctc ttctataatg acttccaggc agatgacgcc gacctgtccg actataccaa
    19381 caagagttat ggtacagacg tgacgttggg cttcccgatc aacgaatata actcgctgcg
    19441 tgcaggtctg ggttatgtac ataactccct gtccaacatg cagcctcagg ttgcgatgtg
    19501 gcgttatctg tactctatgg gtgaacatcc gagcacctct gatcaggata acagcttcaa
    19561 aacggacgac ttcacgttca actatggttg gacctataac aagcttgacc gtggttactt
    19621 cccgacagat ggttcacgtg tcaacctgac cggtaaagtg accattcctg gatcggataa
    19681 cgaatactac aaagtgacgt tagacacggc gacttatgtg ccgatcgatg acgatcacaa
    19741 atgggttgtt ctggggcgta cccgctgggg ttatggtgat ggtttaggcg gcaaagagat
    19801 gccgttctac gagaacttct atgccggtgg ttccagcacc gtgcgtggct tccagtccaa
    19861 tacaattggt ccgaaagcag tttacttccc gcatcaggcc agtaattatg atccggacta
    19921 tgattacgaa tgtgcgactc aggacggcgc gaaagacctg tgtaaatcgg atgatgctgt
    19981 aggcggtaac gccatggcgg ttgccagcct cgagttcatc accccgacgc cgtttattag
    20041 cgataagtat gctaactcgg ttcgtacttc cttcttctgg gatatgggta ccgtttggga
    20101 tacaaactgg gattccagcc aatattctgg atatccggac tatagtgatc caagcaatat
    20161 ccgtatgtct gcgggtatcg cattacaatg gatgtcccca ttggggccgt tggtgttctc
    20221 ctacgcccag ccgttcaaaa agtacgatgg agacaaggca gaacagttcc agtttaacat
    20281 cggtaaaacc tggtaagtgt tctccacaaa ggaatgtagt ggtagtgtag cgatgacttt
    20341 aggcgatcaa tataagatcg ccgggccacg caaagaactg caccctccgg tgcaaatggg
    20401 atggtaagga gtttattgtg aaaaagtggt tattagcggc aggtctcggt ttagcactgg
    20461 caacttctgc tcaggcggct gacaaaattg caatcgtcaa tatgggcagc ctgttccagc
    20521 aggtagcgca gaaaaccggt gtttctaaca cgctggaaaa tgagttcaaa ggccgtgcca
    20581 gcgaactgca gcgtatggaa accgatctgc aggctaaaat gaaaaagctg cagtccatga
    20641 aagcgggcag cgatcgcact aagctggaaa aagacgtgat ggctcagcgc cagacttttg
    20701 ctcagaaagc gcaggctttt gagcaggatc gcgcacgtcg ttccaacgaa gaacgcggca
    20761 aactggttac tcgtatccag actgctgtga aatccgttgc caacagccag gatatcgatc
    20821 tggttgttga tgcaaacgcc gttgcttaca acagcagcga tgtaaaagac atcactgccg
    20881 acgtactgaa acaggttaaa taagtaatgc cttcaattcg actggctgat ttagcgcagc
    20941 agttggatgc agaactacac ggtgatggcg atatcgtcat caccggcgtt gcgtccatgc
    21001 aatctgcaca aacaggtcac attacgttca tggttaaccc aaaataccgt gagcatttag
    21061 gcttgtgcca ggcgtccgcg gttgtcatga cccaggacga tcttcctttc gcgaaaagtg
    21121 ccgcgctggt agtgaagaat ccctacctga cttacgcgcg catggcgcaa attttagata
    21181 ccacgccgca gcccgcgcag aacattgcac ccagtgcggt gatcgacgcg acggcgaagc
    21241 tgggtaacaa cgtatcgatt ggcgctaacg cggtgattga gtccggcgtt gaactgggcg
    21301 ataacgtgat tatcggtgcc ggttgcttcg taggtaaaaa cagcaaaatc ggtgcaggtt
    21361 cgcgtctctg ggcgaacgta accatttacc atgagatcca gatcggtcag aattgcctga
    21421 tccagtccgg aacagtggta ggcgcagacg gctttggtta tgccaacgat cgtggtaact
    21481 gggtgaagat cccacagatt ggtcgcgtaa ttattggcga tcgcgtggag atcggtgcct
    21541 gcacaaccat cgatcgcggc gcgctggatg acactgttat tggcaatggc gtgatcattg
    21601 ataaccagtg ccagattgca cataacgtcg tgattggcga caatacggcg gttgccggtg
    21661 gcgtcattat ggcgggcagc ctgaaaattg gtcgttactg catgatcggc ggagccagcg
    21721 taatcaacgg gcatatggaa atatgcgaca aagtgacggt tacgggcatg ggtatggtga
    21781 tgcgtcccat cactgaacca ggcgtctatt cctcaggcat tccgctgcaa cccaacaaag
    21841 tctggcgcaa aaccgctgca ctggtgatga acattgatga catgagcaag cgtctgaaat
    21901 cgcttgagcg caaggttaat caacaagact aacgttccat cttttgttcg ccaaacttta
    21961 cggcctgtct cattcttacg attgcggcag gccgtgttat tattgtcgtt tcttatattt
    22021 tgacaggaag agtatcttga ctactaacac tcatactctg cagattgaag agattttaga
    22081 acttctgccg caccgtttcc cgttcttact ggtggatcgc gtgctggatt ttgaagaagg
    22141 tcgttttctg cgcgcagtaa aaaatgtctc tgtcaatgag ccattcttcc agggccattt
    22201 ccctggaaaa ccgattttcc cgggtgtgct gattctggaa gcaatggcac aggcaacagg
    22261 tattctggcg tttaaaagcg taggaaaact ggaaccgggt gagctgtact acttcgctgg
    22321 tattgacgaa gcgcgcttca agcgcccggt cgtgcctggc gatcaaatga tcatggaagt
    22381 cactttcgaa aaaacgcgcc gcggcctgac ccgttttaaa ggggttgctc tggtcgatgg
    22441 taaagtagtt tgcgaagcaa cgatgatgtg tgctcgtagc cgggaggcct gatacgtgat
    22501 tgataaatcc gcctttgtgc atccaaccgc cattgtggaa gagggcgcgt caattggcgc
    22561 gaacgcacac attggtcctt tttgtatcgt tggaccccat gtcgaaattg gtgagggtac
    22621 cgtactgaaa tctcacgttg tcgtgaatgg tcatactaaa attggccgcg ataatgagat
    22681 ttatcagttc gcctccatcg gcgaagttaa ccaggatctg aaatatgctg gcgaaccgac
    22741 ccgtgtggaa atcggcgatc gtaaccgcat tcgcgaaagc gtcaccattc atcgtggcac
    22801 agtccagggc ggtggattga cgaaggtggg cagcgacaac ttactgatga tcaacgcgca
    22861 cattgcgcac gattgtacgg taggtaaccg ctgtattctc gccaacaacg caacgctggc
    22921 gggtcacgta tcggttgacg acttcgcgat catcggcggc atgaccgcag tccatcagtt
    22981 ctgcatcatt ggtgcgcacg tgatggttgg cggctgctcc ggtgtggcgc aggacgtccc
    23041 tccttatgtc attgcgcagg gtaaccacgc aacgccgttc ggtgtcaata tcgaagggct
    23101 gaagcgccgc ggattcagcc gtgaggcgat taccgctatc cgcaatgcgt ataagctgat
    23161 ttatcgtagc ggtaaaacgc tcgatgaagt gaaaccggaa attgctgaac tggcggaaac
    23221 atatccggaa gtgaaagcct ttaccgattt ctttgcacgc tcaacgcgcg gtctgattcg
    23281 ttaatgactg aacagcgtcc attaacgatt gccctggtcg ccggagaaac ctccggcgat
    23341 atcctggggg ccggtttaat ccgcgctctg aaagaacatg tgcccaacgc ccgctttgtt
    23401 ggtgttgccg ggccacgaat gcaggctgaa ggctgcgaag cttggtacga aatggaagaa
    23461 ctggcggtaa tgggcattgt tgaagtgctc ggtcgtctgc gtcgcttact gcatattcgt
    23521 gccgatctga caaagcgttt tggcgaactg aagccagatg tttttgttgg tattgatgcg
    23581 cccgacttca atattactct tgaaggtaac ctcaaaaagc agggtatcaa aaccattcat
    23641 tacgtcagtc catccgtctg ggcgtggcga cagaaacgcg ttttcaaaat aggcagagcc
    23701 accgatctgg tgctcgcatt tctgcctttc gaaaaagcgt tttatgacaa atacaatgta
    23761 ccgtgccgct ttatcggtca taccatggct gatgccatgc cgttagatcc agataaaaat
    23821 gccgcccgtg atgtgctggg gatccctcac gatgcccact gcctggcgtt gctaccgggg
    23881 agccgtggtg cagaagttga aatgcttagt gccgatttcc tgaaaacggc ccagcttttg
    23941 cgccagacat atccggatct cgaaatcgtg gtgccactgg tgaatgccaa acgccgcgag
    24001 cagtttgaac gcatcaaagc tgaagtcgcg ccagaccttt cagttcattt gctggatggg
    24061 atgggccgtg aggcgatggt cgccagcgat gcggcgctac tggcgtcggg tacggcagcc
    24121 ctggagtgta tgctggcgaa atgcccgatg gtggtgggat atcgcatgaa gccttttacc
    24181 ttctggttgg cgaagcggct ggtgaaaact gattatgtct cgctgccaaa tctgctggcg
    24241 ggcagagagt tagttaaaga attattgcag gaagagtgtg agccgcaaaa actggctgcg
    24301 gcgctgttac cgctgttggc gaacgggaaa accagccacg cgatgcacga taccttccgt
    24361 gaactgcatc agcagatccg ctgcaatgcc gatgagcagg cggcacaagc cgttctggag
    24421 ttagcacaat gatcgaattt gtttatccgc acacgcagct ggttgcgggt gtggatgaag
    24481 tcggacgcgg gccgttagtt ggcgcggtcg tcaccgctgc ggtgatcctt gacccggcgc
    24541 gcccgattgc cgggctgaat gattccaaaa agctgagcga aaaacgccgt ctggcgctct
    24601 atgaagagat caaagagaaa gcgttgagct ggagtctggg ccgcgcggaa ccccacgaaa
    24661 tcgacgagct gaacattctt catgcgacca tgctggcgat gcagcgtgcc gtcgctgggc
    24721 tgcatattgc gccggaatat gtgttgattg atggtaaccg ctgcccgaaa ttaccgatgc
    24781 ctgcgatggc tgtggtgaaa ggcgatagcc gcgtaccgga aatcagtgcc gcgtctatcc
    24841 tggcgaaagt gacgcgtgac gccgaaatgg cggcgctgga tattgttttc ccgcaatatg
    24901 gttttgccca acacaaaggg tacccaaccg cttttcatct ggaaaaactg gctgaacacg
    24961 gcgcgaccga acaccatcgg cgcagctttg ggcctgtcaa acgcgcactg ggacttgcgt
    25021 cctgattctt gtgtcgagat taagtaaacc ggaatctgaa gatgtctgaa ccacgtttcg
    25081 tacacctgcg ggtgcacagc gactactcga tgatcgatgg cctggccaaa accgcaccgt
    25141 tggtaaaaaa ggcggcggcg ttgggtatgc cagcactggc gatcaccgat ttcaccaacc
    25201 tttgtggtct ggtgaagttc tacggagcgg gacatggcgc agggattaag cctatcgtcg
    25261 gggcagattt taacgtccag tgcgacctgc tgggtgatga gttaacccac ctgacggtac
    25321 tggcggcgaa caataccggc tatcagaatc tgacgttgct gatctcaaaa gcgtatcagc
    25381 gcgggtacgg tgccgccggg ccgatcatcg atcgcgactg gcttatcgaa ttaaacgaag
    25441 ggttgatcct tctttccggc ggacgcatgg gcgacgtcgg acgcagtctt ttgcgtggta
    25501 acagcgcgct ggtagatgag tgtgtcgcgt tttatgaaga acacttcccg gatcgctatt
    25561 ttctcgagct gatccgcacc ggcaggccgg acgaagaaag ctatctgcac gcggcggtgg
    25621 aactggcgga agcgcgcggt ttgcccgtcg tggcgaccaa cgacgtgcgc tttatcgaca
    25681 gcagcgactt tgacgcacac gaaatccgcg tcgcgatcca cgacggcttt accctcgacg
    25741 atcctaaacg cccgcgtaac tattcgccgc agcaatatat gcgtagcgaa gaggagatgt
    25801 gcgagctgtt tgccgacatc cccgaagctc ttgccaacac cgttgagatc gccaaacgtt
    25861 gtaacgtaac cgtgcgtctt ggtgaatact tcctgccgca gttcccgacc ggggacatga
    25921 gcaccgaaga ttatctggtc aagcgtgcaa aagagggcct ggaagagcgt ctggcctttt
    25981 tattccctga cgaggaagaa cgtgttaagc gccgcccgga atatgacgaa cgtctggaga
    26041 ctgaacttca ggttatcaac cagatgggct tcccgggcta cttcctcatc gttatggaat
    26101 ttatccagtg gtcgaaagat aacggcgtac cggtagggcc aggccgtggc tccggtgcgg
    26161 gttcactggt ggcctacgcg ctgaaaatca ccgacctcga tccgctggaa tttgacctgc
    26221 tgttcgaacg tttccttaac ccggaacgtg tctccatgcc tgacttcgac gttgacttct
    26281 gtatggagaa acgcgatcag gttatcgagc acgtagcgga catgtacggt cgtgatgcgg
    26341 tatcgcagat catcaccttc ggtacaatgg cggcgaaagc ggtgatccgc gacgtaggcc
    26401 gcgtgctggg gcatccgtac ggctttgtcg atcgtatctc gaaactgatc ccgcccgatc
    26461 cggggatgac gctggcgaaa gcgtttgaag ccgagccgca gctgccggaa atctacgaag
    26521 cggatgaaga agttaaggcg ctgatcgaca tggcgcgcaa actggaaggg gtcacccgta
    26581 acgccggtaa gcacgccggt ggggtggtta tcgcgccgac caaaattacc gattttgcgc
    26641 cgctttactg cgatgaagag ggcaaacatc cggtcaccca gtttgataaa agcgacgttg
    26701 aatacgccgg actggtgaag ttcgacttcc ttggtttgcg tacgctcacc atcatcaact
    26761 gggcgctgga gatgatcaac aagcggcggg cgaagaatgg cgagccgccg ctggatatcg
    26821 ctgcgatccc gctggatgat aagaaaagct tcgacatgct gcaacgctcg gaaaccacgg
    26881 cggtattcca gcttgaatcg cgcggcatga aggacctgat caagcgtctg caacctgact
    26941 gcttcgaaga tatgatcgca ctggtggcac tgttccgccc cggcccgttg cagtcaggga
    27001 tggtggataa ctttatcgac cgtaaacatg gtcgcgaaga gatttcctat ccggacgtac
    27061 agtggcagca tgaaagcctg aaaccggtac tggagccaac ctacggcatc atcctgtatc
    27121 aggaacaggt catgcagatt gcgcaggtgc tttctggtta taccctcggt ggcgcggata
    27181 tgctgcgtcg tgcgatgggt aagaaaaagc cggaagagat ggctaagcag cgttctgtat
    27241 ttgctgaagg tgcagaaaag aacggaatca acgccgaact ggcgatgaaa atcttcgacc
    27301 tggtggagaa attcgcgggt tacggattta acaaatcgca ctctgcggcc tatgctttgg
    27361 tgtcatatca aacgttatgg ctgaaagcgc actatccggc ggagtttatg gcggcggtaa
    27421 tgaccgccga tatggacaac accgagaagg tggtgggcct ggtggatgag tgctggcgga
    27481 tggggctgaa aatcctgcca ccagatataa actccggtct ttaccatttc cacgtcaacg
    27541 acgacggcga aatcgtgtat ggtattggcg ccatcaaagg ggtaggtgaa ggtccgattg
    27601 aggccatcat cgaagcccgt aataaaggcg gctacttccg cgaactgttt gatctctgcg
    27661 cccgtaccga caccaaaaag ttaaaccggc gggtgctgga aaaactgatc atgtccgggg
    27721 cgtttgaccg tcttgggcca caccgcgcgg cgctgatgaa ctcgctgggt gatgcgttaa
    27781 aagcggcaga tcaacacgcg aaagcggaag ctatcggtca ggccgatatg ttcggcgtgc
    27841 tggccgaaga gccggaacaa attgaacaat cctacgccag ctgccaaccg tggccggagc
    27901 aggtggtgtt agatggggaa cgtgaaacgt taggtctgta ccttacggga caccctatca
    27961 accagtattt aaaagagatt gagcgttatg tcggaggcgt aaggctgaaa gacatgcacc
    28021 cgacagaacg tggtaaagtc atcacggctg cggggctcgt tgttgccgcg cgggttatgg
    28081 tcaccaagcg cggcaatcgt atcggtatct gcacgctgga tgaccgttcc gggcggctgg
    28141 aagtgatgtt gtttactgac gccctggata aataccagca attgctggaa aaagaccgca
    28201 tacttatcgt cagcggacag gtcagctttg atgacttcag cggtgggctt aaaatgaccg
    28261 ctcgcgaagt gatggatatt gacgaagccc gggaaaaata tgctcgcggg cttgctatct
    28321 cgctgacgga caggcaaatt gatgaccagc ttttaaaccg actccgtcag tctctggaag
    28381 cccaccgctc tgggacaatt ccagtacatc tctactatca gagggcggat gcacgcgcgc
    28441 ggttgcgttt tggcgcgacg tggcgtgtct ctccgagcga tcgtttatta aacgatctcc
    28501 gtggcctcat tggttcggag caggtggaac tggagtttga ctaatacagg aatactatga
    28561 gtctgaattt ccttgatttt gaacagccga ttgcagagct ggaagcgaaa atcgattctc
    28621 tgactgcggt tagccgtcag gatgagaaac tggatattaa catcgatgaa gaagtgcatc
    28681 gtctgcgtga aaaaagcgta gaactgacac gtaaaatctt cgccgatctc ggtgcatggc
    28741 agattgcgca actggcacgc catccacagc gtccttatac cctggattac gttcgcctgg
    28801 catttgatga atttgacgaa ctggctggcg accgcgcgta tgcagacgat aaagctatcg
    28861 tcggtggtat cgcccgtctt gacggtcgtc cggtgatgat cattggtcat caaaaaggtc
    28921 gtgaaaccaa agaaaaaatt cgccgtaact ttggtatgcc agcgccagaa ggttaccgca
    28981 aagcactgcg tctgatgcaa atggctgaac gctttaagat gcctatcatc acctttatcg
    29041 acaccccggg ggcttatcct ggcgtgggcg cagaagagcg tggtcagtct gaagccattg
    29101 cacgcaacct gcgtgaaatg tctcgcctcg gcgtaccggt agtttgtacg gttatcggtg
    29161 aaggtggttc tggcggtgcg ctggcgattg gcgtgggcga taaagtgaat atgctgcaat
    29221 acagcaccta ttccgttatc tcgccggaag gttgtgcgtc cattctgtgg aagagcgccg
    29281 acaaagcgcc gctggcggct gaagcgatgg gtatcatcgc tccgcgtctg aaagagctga
    29341 aactgatcga ctccatcatc ccggaaccgc tgggcggcgc tcaccgtaac ccggaagcga
    29401 tggcggtatc gttgaaagcg caactgctgg cggatctggc cgatctcgat gtgttaagca
    29461 ctgaagattt aaaaaatcgt cgttatcagc gcctgatgag ctacggttac gcgtaattcg
    29521 caaaagttct gaaaaagggt cacttcgg
//
LOCUS       DAJTZL010000035        28709 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.035, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000035 DAJTZL010000000
VERSION     DAJTZL010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28709
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.035"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(301..507)
                     /locus_tag="OO544_001359"
     CDS             complement(301..507)
                     /locus_tag="OO544_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HCR6766995.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            820..909
                     /gene="kdpF"
                     /locus_tag="OO544_001360"
     CDS             820..909
                     /gene="kdpF"
                     /locus_tag="OO544_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HCR6766996.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            909..2582
                     /gene="kdpA"
                     /locus_tag="OO544_001361"
     CDS             909..2582
                     /gene="kdpA"
                     /locus_tag="OO544_001361"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HCR6766997.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFAFIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGALQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2605..4653
                     /gene="kdpB"
                     /locus_tag="OO544_001362"
     CDS             2605..4653
                     /gene="kdpB"
                     /locus_tag="OO544_001362"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HCR6766998.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGAMPGNALFSAAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVDEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHIEANGGHFPADVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAATYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            4662..5234
                     /gene="kdpC"
                     /locus_tag="OO544_001363"
     CDS             4662..5234
                     /gene="kdpC"
                     /locus_tag="OO544_001363"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HCR6766999.1"
                     /translation="MSGLRPALSTFLFLLLITGGVYPLLTTALGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKLIAARV
                     AALRAANPDASASIPVELVTASASGLDNNITPQAAAWQIPRIAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5227..7911
                     /gene="kdpD"
                     /locus_tag="OO544_001364"
     CDS             5227..7911
                     /gene="kdpD"
                     /locus_tag="OO544_001364"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HCR6767000.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLEGLAVLPPKRLAYRGRHICEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPSEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSPQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANALERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGIDAHFEGENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEDFHEDM"
     gene            7908..8585
                     /gene="kdpE"
                     /locus_tag="OO544_001365"
     CDS             7908..8585
                     /gene="kdpE"
                     /locus_tag="OO544_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HCR6767001.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVFEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSPVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGYRFML"
     gene            8860..8964
                     /locus_tag="OO544_001366"
     CDS             8860..8964
                     /locus_tag="OO544_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2618 domain-containing protein"
                     /protein_id="HCR6767002.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            9275..11473
                     /gene="speF"
                     /locus_tag="OO544_001367"
     CDS             9275..11473
                     /gene="speF"
                     /locus_tag="OO544_001367"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HCR6767003.1"
                     /translation="MSELKIAVSRSCPDCFSTHRACVNIDESNYIDVAAIILSVNDVE
                     RGKLDEIDATGYGIPVFIATENEERVPAEYLPRISGVFEHCESRKEFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGETLFRSDLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELIAEVAPQR
                     AKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCS
                     PLLLDLNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMH
                     ASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELV
                     DGKPWQSYETAQIAVDLRFFQFVPGEHWHSFEGYAENQYFVDPCKLLLTTPGIDARNG
                     EYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLL
                     ESDAPLAEVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQLQKEMFRKAHFPHV
                     SMNPQEANYAYLRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFS
                     ALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSTLLKGEKL"
     gene            11470..12789
                     /gene="potE"
                     /locus_tag="OO544_001368"
     CDS             11470..12789
                     /gene="potE"
                     /locus_tag="OO544_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HCR6767004.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(12845..14485)
                     /gene="pgm"
                     /locus_tag="OO544_001369"
     CDS             complement(12845..14485)
                     /gene="pgm"
                     /locus_tag="OO544_001369"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HCR6767005.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGHVKEQDLVQQFVEGLADI
                     VDMAAIQKTGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(14511..15056)
                     /gene="seqA"
                     /locus_tag="OO544_001370"
     CDS             complement(14511..15056)
                     /gene="seqA"
                     /locus_tag="OO544_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HCR6767006.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            15241..16005
                     /gene="ybfF"
                     /locus_tag="OO544_001371"
     CDS             15241..16005
                     /gene="ybfF"
                     /locus_tag="OO544_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR6767007.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPRDPVMNYPAMAQDLVDTLDAQQIDKATFIGHSMGGKAVMALTALA
                     RDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMHQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            16145..16438
                     /gene="ybfE"
                     /locus_tag="OO544_001372"
     CDS             16145..16438
                     /gene="ybfE"
                     /locus_tag="OO544_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HCR6767008.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            16578..17108
                     /gene="fldA"
                     /locus_tag="OO544_001373"
     CDS             16578..17108
                     /gene="fldA"
                     /locus_tag="OO544_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HCR6767009.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            17318..17404
                     /gene="uof"
                     /locus_tag="OO544_001374"
     CDS             17318..17404
                     /gene="uof"
                     /locus_tag="OO544_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HCR6767010.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            17397..17843
                     /gene="fur"
                     /locus_tag="OO544_001375"
     CDS             17397..17843
                     /gene="fur"
                     /locus_tag="OO544_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HCR6767011.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(17928..18254)
                     /locus_tag="OO544_001376"
     CDS             complement(17928..18254)
                     /locus_tag="OO544_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6767012.1"
                     /translation="MKKLILIAIMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQHFADQESVRVLDYQQYLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(18304..19710)
                     /gene="chiP"
                     /locus_tag="OO544_001377"
                     /pseudo
     CDS             complement(18304..19710)
                     /gene="chiP"
                     /locus_tag="OO544_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
     gene            complement(20288..21952)
                     /gene="glnS"
                     /locus_tag="OO544_001378"
     CDS             complement(20288..21952)
                     /gene="glnS"
                     /locus_tag="OO544_001378"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HCR6767013.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVESIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(22211..24157)
                     /gene="nagE"
                     /locus_tag="OO544_001379"
     CDS             complement(22211..24157)
                     /gene="nagE"
                     /locus_tag="OO544_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HCR6767014.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLTQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDAMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVSDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGNVVAGQTPLYEIKK"
     gene            24490..25290
                     /gene="nagB"
                     /locus_tag="OO544_001380"
     CDS             24490..25290
                     /gene="nagB"
                     /locus_tag="OO544_001380"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCR6767015.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            25350..26498
                     /gene="nagA"
                     /locus_tag="OO544_001381"
     CDS             25350..26498
                     /gene="nagA"
                     /locus_tag="OO544_001381"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCR6767016.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLARHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTSFTPDFKITKTIVNGNEVVTQ"
     gene            26507..27727
                     /gene="nagC"
                     /locus_tag="OO544_001382"
                     /gene_synonym="nagR"
     CDS             26507..27727
                     /gene="nagC"
                     /locus_tag="OO544_001382"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HCR6767017.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            27775..28527
                     /gene="nagD"
                     /locus_tag="OO544_001383"
     CDS             27775..28527
                     /gene="nagD"
                     /locus_tag="OO544_001383"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HCR6767018.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
BASE COUNT         6731 a         7315 c         7522 g         7141 t
ORIGIN      
        1 acaatgctac cgccatactg cgtcatgtcg ccctgacgcg ccgccggttt tccgctcatt
       61 tctcatcctt gttttatcgg gttatttgtc cgctgacagg caagacccta cagatttata
      121 agaaagcctt aagtgagata tccgtcagat agaaacaatt aggaaaatat ttatatatat
      181 ttcatgaata tatgtttcga ttggtataac ggtgtctaaa tattcctctg aacatcttcc
      241 tgatacaaaa aaccgccagc gtttccgctg gcggttttca atgtattcag aactcagcaa
      301 tcagtaaaaa tcaccggttg ccttttcggc ctgatccatc cacaccggtt tatcgctggt
      361 tttcgaccag acgcggtgca ggtagctgta aaagcgggcg cggtctttcc agaaaagcat
      421 gaaaggcagc gccagaacgc ccgctgcaac cgcatatgta cggcgtaaaa agataagcca
      481 tgcaggatat tctctgtaga gttccatact tttctccccc ttttgtatct acccggtgaa
      541 tagcaccgga aaaatgaatt tgtttatctg atgaaaatag taccgccttt tgtgtaattt
      601 tactactcat ccgaccactt atttttgctt attgatggtt tatttacatt cactctgtaa
      661 ttaagttaca caaaagttaa attaatacta aacattagtt aaatcatggc ttttgccatt
      721 tttatacttt ttttacaccc cgcccgcaga tttttgcgaa atctttgcag ccagaattct
      781 actcttccgg tatcactttt aggccactgg aggtgcactg tgagtgcagg cgtgatagcc
      841 ggcgtgttgc tggtgttttt attactgggt tatttggttt atgccctgat caatgcggag
      901 gcgttctgat ggctgcgcaa gggttcttac tgattgccac gtttttactg gtattaatgg
      961 tgctggcgcg tcctttaggc agcgggctgg cgcggctgat taatgacatt cctcttccag
     1021 gtacagcggg cgttgaacgt atactctttc gtttacctgg cgtctctgac catgagatga
     1081 actggaagca atatctttgt gccattctcg gcctgaacat gctggggctg gcggtgctgt
     1141 tttttatgtt gctcggtcag cactatctgc cgcttaatcc acagcagttg ccggggctgt
     1201 cgtgggatct ggcgctgaat accgccgtca gctttgtcac gaataccaac tggcaatctt
     1261 atagcggtga aaccacgttg agctatttca gccagatggc aggcttaacg gtacaaaact
     1321 ttctttctgc cgccagcggg attgcggtga tttttgcctt catccgtgcg tttactcgcc
     1381 agagcatgag cacactcggg aatgcctggg tcgatctgct acgcatcacg ttatgggtgc
     1441 tagtccctgt ggcgttgttg atcgcactgt tttttattca acaaggtgcg ctgcaaaact
     1501 ttctgcccta tcaggcagtg aataccgttg aaggagcgca acagctgtta cccatgggac
     1561 cagtagcttc tcaggaagcg atcaagatgc tcggtactaa cggcggtggc ttctttaatg
     1621 ccaactcgtc gcatccgttt gaaaacccaa ccgcactgac caacttcgtg cagatgctgg
     1681 cgatcttctt gatcccaaca gcgctgtgct ttgcctttgg tgaagtgacg ggcgatcgcc
     1741 gccaggggcg catgttgctg tgggcgatgt cagtgatttt tgtcatctgc gtaggcgtgg
     1801 ttatgtgggc agaagttcag ggtaatcctc atctgctggc gctcggcgca gacagcagca
     1861 tcaatatgga aggtaaagag agccgtttcg gcgtgctggt cagtagcctg tttgcggtcg
     1921 tgactacggc ggcgtcctgt ggcgcggtga ttgcgatgca tgattcgttt accgctctcg
     1981 gcggcatggt gccgatgtgg ctaatgcaaa ttggtgaagt ggtgttcggc ggtgtcggtt
     2041 ctggtcttta cggcatgatg ctgtttgtcc tgctggcggt gtttattgcc gggctgatga
     2101 ttggtcgtac accggaatat ctgggtaaaa aaatcgacgt acgcgagatg aaactgactg
     2161 cactggcgat tctggttact ccaacactgg tactgatggg cgcggcgttg gcgatgatga
     2221 ccgacgccgg acgtagcgcc atgcttaacc ctggcccgca tggttttagc gaagtgctgt
     2281 acgccgtgtc gtccgccgct aacaacaacg gcagcgcctt tgccggatta agcgccaact
     2341 ctccgttctg gaactgttta ctggcgttct gcatgtttgt cggtcgcttc ggggtgatta
     2401 tcccggtgat ggcaattgcc ggttcgctgg tgagtaaaaa gagccaaccc gccagctccg
     2461 gcacgctgcc aacgcacggc ccgctgtttg ttggcctgtt aatcggcacc gtgttgctgg
     2521 ttggcgcact gacctttatc cctgccctgg cgcttggtcc ggtggcggaa tatctctcct
     2581 gatgatattg agcgagcact gaatatgagt cgtaaacaac tggcgctatt cgaaccaaca
     2641 cttgtcgttc aggcgctgaa agaagcggtg aaaaaattaa acccgcaggc gcaatggcgc
     2701 aatccggtga tgtttatcgt ctggatcggc agtctgctga ccacctgtat tagcatcgcg
     2761 atggcaagcg gtgcgatgcc tggcaatgcg ctgtttagcg cggcaattag cggctggctg
     2821 tgggtcaccg tactgttcgc taatttcgcc gaggcgctgg cagaaggccg cagtaaagcg
     2881 caggccaaca gtcttaaagg ggtgaaaaaa actgcctttg cccgcaagct gcgtgagccg
     2941 aaatatggcg ctgcggcgga caaagttcct gccgatcaac tgcgtaaagg tgacatcgtg
     3001 ctggtagaag ctggcgatat tatcccctgc gatggtgaag tcattgaagg cggcgcatcg
     3061 gtcgatgaaa gcgccatcac cggggaatcg gcaccggtga tccgtgaatc cggcggcgat
     3121 tttgcctccg tcaccggcgg cacgcgtatt ctttctgact ggctggtgat tgagtgtagc
     3181 gttaaccccg gtgagacatt tctggatcgg atgatcgcga tggtggaagg cgcacagcga
     3241 cgcaaaacac cgaacgagat tgccctgact atcctgctga ttgctctgac catcgtcttt
     3301 ttactggcaa ccgccacgct gtggccgttt tccgcgtggg gcggtaatgc cgtgagtgta
     3361 acggtactgg tggcgctgct ggtctgtctg atcccaacca ctattggcgg cctgttgtca
     3421 gcgatcggcg tcgccgggat gagtcggatg ctgggcgcga atgtgattgc caccagcgga
     3481 cgtgcagttg aagcggcagg tgacgttgac gttctgctac tggataaaac cggcaccatc
     3541 acactcggta accgtcaggc gtcggagttt atccccgcgc agggcgtgga tgaaaaaacg
     3601 ctggctgacg ccgcacaact ggcttcgctg gctgatgaaa cgccggaagg tcgcagtatt
     3661 gtgatcctcg ccaagcaacg ttttaacctg cgcgagcgcg atgtgcagtc gctccatgcc
     3721 acctttgtac cgtttactgc gcaaagccgg atgagcggga tcaacatcga caaccgcatg
     3781 atccgtaaag gttctgtcga tgccattcgc cgccatattg aggctaacgg cggtcacttc
     3841 cctgccgatg ttgatcaaaa agtcgatcag gttgcgcgtc agggagccac gccgctggtg
     3901 gtggtggaag gttctcgtgt gctgggcgtt attgcgctga aagatatcgt caaaggcggt
     3961 attaaagagc gcttcgccca gctgcgcaaa atgggcatta aaacggtgat gattaccggc
     4021 gataaccgtc tgactgccgc cgcgatagct gcggaagcgg gtgtcgatga ttttctcgcc
     4081 gaagcaacac cggaggccaa gctggcattg atccgtcagt atcaggcgga aggtcgtttg
     4141 gtagcgatga ccggcgacgg caccaacgat gctccggcgc tggcgcaggc agatgtcgcg
     4201 gtggcgatga actccggcac ccaggcggcg aaagaggcgg gcaatatggt ggatctcgac
     4261 tctaacccga ccaagttgat cgaggtggtg cacattggca aacagatgct gatgacccgt
     4321 ggctcgctga ccaccttcag cattgccaac gatgtggcga aatacttcgc cattattccg
     4381 gcggcgttcg cggcaacata tccgcagtta aatgcgctga acatcatgcg cctgcattcg
     4441 cccgactccg ccattctcag tgcggtcatt ttcaacgcct tgattatcgt ctttttgatt
     4501 cctctggcgt taaaaggcgt gagttataaa ccgcttaccg cttctgccat gttgcgccgt
     4561 aacttatgga tttacggtct gggtgggctg ctggtgccgt ttattggtat caaagtcatt
     4621 gatttactgc tgaccgtttg cggtctggtg tgaggtttac catgagtgga ttacgtccgg
     4681 cattatcgac atttctcttt ctgttattga ttactggcgg cgtttacccg ctgctgacca
     4741 ccgcactggg gcaatggtgg tttccctggc aggccaacgg ttcgttgatc cgtgaaggtg
     4801 atacggtgcg tggctcggca ttaatcgggc agaattttac cggcaacggc tattttcatg
     4861 gtcgcccgtc agcaacggct gaaatgccct ataatccaca ggcttctggc gggagcaatc
     4921 tggcggtcag taaccctgag ctggataaac taatagccgc acgcgttgct gcattacggg
     4981 ccgctaaccc ggatgccagc gcgagcattc cggttgaact ggtgacggca tcggcaagcg
     5041 ggctggacaa taatatcacc ccgcaagcgg cggcctggca gatcccacgc atcgcgaaag
     5101 cgcgtaatct cagcgttgaa cagctcacgc agctgatcgc aaaatacagc caacaaccgc
     5161 tggtgaaata tatcggccag ccggtcgtca acattgtcga actcaatctg gcgctggata
     5221 agcttgatga ataacgaacc cttacgtccc gaccccgatc gtctgctgga acaaactgcc
     5281 gcgccgcatc gggggaagct gaaagttttc ttcggtgcct gtgcaggcgt cgggaagacc
     5341 tgggcgatgc tggcagaagc ccagcgactg cgggcgcaag ggctggatat tgtggttggc
     5401 gtggtagaaa cccacgggcg aaaagatacc gccgcaatgc tggaagggct ggctgttctg
     5461 ccgccaaaac gcctggcgta ccgtgggcgg cacatctgcg agtttgacct cgatgccgcc
     5521 ctcgcccgcc gtccggcgct gatcttaatg gacgaactgg cgcacagtaa tgcgccaggt
     5581 tctcgtcacc ctaaacgctg gcaggacatc gaagagctgc tggaagcagg cattgatgtt
     5641 ttcactaccg tcaacgttca gcatctggaa agtctgaatg atgtggtcag cggcgtcacc
     5701 ggaattcagg tacgggaaac cgtgcccgat ccttttttcg atgccgccga cgacgtggtt
     5761 ctggtggact tgcccccgga cgatctgcgc cagcggctga aagaaggcaa agtctatatt
     5821 gccgggcagg cggagcgcgc cattgaacat tttttccgca aaggcaatct gatcgccctg
     5881 cgcgaactgg cactgcgccg tactgccgat cgcgttgatg agcaaatgcg cgcctggcgg
     5941 gggcatccta gcgaagagaa agtctggcac acgcgcgacg cgatcctttt atgcatcggc
     6001 cataacaccg gtagtgagaa gctggtccgc gcggcggcgc ggctggcgtc acggctgggt
     6061 agcgtctggc acgcggtgta tgttgaaacc cctgccctgc atcgcttacc ggagaaaaaa
     6121 cgtcgtgcaa ttctcagcgc cttacgtctg gcgcaggaac tgggcgcgga gacggcaaca
     6181 ctttctgatc cagcggaaga gaaagcggta gtgcgttatg cccgtgaaca taatctcggc
     6241 aagattattc tcggtcgccc ggcctcgcgc cgctggtggc gtcgggaaac gtttgctgac
     6301 cgactggcgc gcatcgcccc cgatctcgat caggtgctgg tcgcgcttga tgaaccaccc
     6361 gcccgcacga ttaacaacgc gccggatagc cgctctttta aagacaagtg gcgggtacag
     6421 attcagggat gcgtggttgc cgccgcgtta tgcgccgtta tcaccttaat tgccatgcag
     6481 tggctgatgg cgtttgatgc cgccaacctg gtgatgctgt atctgcttgg cgtggtggtg
     6541 gtggcgctat tttatggacg ctggccttcg gtggttgcca ccgtcattaa tgtggtgagt
     6601 ttcgatctct tttttatcgc cccacgcggc acgctcgccg tctctgatgt gcaatatctg
     6661 ctgaccttcg cggtgatgtt aaccgtcggg ctggtgatcg ggaaccttac tgctggcgtg
     6721 cgttatcagg cgcgggtagc ccgttaccgc gagcaacgca cacggcactt atatgaaatg
     6781 tcgaaagctc tggcggtggg ccgcagtccg caggacatcg ccgccaccag cgaacaattt
     6841 attgcctcca cgtttcatgc ccgcagtcag gtgttgttgc ccgatgacaa cggtaaattg
     6901 cagccgttaa cacatccgca aggaatgacg ccatgggacg atgccatcgc gcagtggagt
     6961 tttgataaag gcctgcctgc gggcgcgggc accgacacgt tacccggtgt accgtaccag
     7021 attttgccgc taaaaagcgg cgagaaaacc tacgggctgg tggtggtgga gccggggaat
     7081 ctgcgccagt tgatgatccc ggaacagcag cgcctgctgg agacgtttac gctgttagtc
     7141 gccaatgccc ttgagcggct gacgctaacc gccagcgaag aacaggcgcg gatggcaagc
     7201 gaacgtgaac agatccgcaa cgccctgctg gcagcgcttt cgcatgattt acgcacgccg
     7261 cttacggtgc tgtttggtca ggcagaaatc ttaacgctcg atctggcaag cgaaggatca
     7321 ccccacgccc gccaggccag cgagattcgt cagcatgtgc tgaacactac ccgactggtg
     7381 aataatctac tggatatggc gcgaattcag tccggcggct ttaatttgaa gaaagagtgg
     7441 ttaacgctgg aagaagtagt cggcagcgcg ctgcaaatgc tggaaccggg tttatcgtcg
     7501 cccatcaatc tttctctgcc agaaccgctg accttaatcc acgttgacgg gccactcttt
     7561 gaacgggtgc tgattaatct gctggagaac gcggtgaaat atgcgggtgc gcaggccgaa
     7621 attggtatcg atgcccactt tgagggcgaa aatctacaac tggatgtctg ggataacggc
     7681 cccggtcttc cgccaggcca ggagcagacg atatttgata agtttgctcg cgggaataaa
     7741 gagtcggcgg tgccgggtgt cgggcttggg ttggcaattt gtcgggcgat tgtggatgta
     7801 cacggcggca ccattaccgc gttcaaccga ccggaaggtg gtgcctgttt tcgtgttaca
     7861 cttccccagc aaaccgctcc tgaacttgaa gactttcatg aggatatgtg acaaacgttc
     7921 tgattgttga agatgaacag gctattcgtc gctttctgcg cacggcgctg gagggcgacg
     7981 ggatgcgcgt ctttgaggcc gaaactctgc aacgcggctt gctggaagcg gcaacccgta
     8041 agccagattt gattattctc gatctcggcc tgcccgatgg tgatgggatt gagtttatcc
     8101 gcgacctgcg ccagtggagc ccggtgccgg tgattgtgct ttccgcacgc agcgaagaga
     8161 gcgacaagat cgccgcgctg gatgccggag cggatgatta tctgagtaag ccgtttggca
     8221 ttggcgaatt gcaggcccgt ctgcgcgtcg ctttacgccg ccactccgcc accgccgcgc
     8281 ccgatccgct ggtgaaattt tccgatgtta ccgtcgattt agccgcccgc gtgattcacc
     8341 ggggtgagga agaggtgcat ctcacaccaa ttgagttccg cctgctggcg gtgctgctca
     8401 acaatgccgg aaaagtactc acccagcgcc agctccttaa ccaggtgtgg gggccaaacg
     8461 cggtcgaaca cagtcactat ttgcgtattt atatgggaca tctgcgacaa aaactggaac
     8521 aggatccggc ccggcctcgc cacttcatta ctgaaaccgg tattggatat cggtttatgc
     8581 tttgaattgt tattcaataa attcgcttaa atagtgatta atttattatt aaacctgcgc
     8641 cgaatattat tatttggcag gtttaatttc ttaacgcaaa tataaaacac gaaattacaa
     8701 catttaagta acaaaacagc atctaatatg gcgatattca taattaatga tttattatct
     8761 ggggtatgat cgttttttgt tgatcttctt cacagattat agccatttca tggatagaat
     8821 aactctacct tcaactgaca cagcaagagg taaaggtaaa tggaaaataa taaccgctta
     8881 atgcctcata taaggcggac aacccatatc atgaagtttg cccatcgtaa tagttttgac
     8941 tttcatttct ttaatgcccg ctagtcttct gactaaaggg caccccaacg tacaggtctc
     9001 cctgacttta agcattacag gttaatacct gtattcctcg gtgctcatat actgctaacc
     9061 cttttaaact ctaaataatt cgagtcgcag cacttgcaac ttgaggtatg acgagtatag
     9121 ccagttaccg ggctggtctg ggttattgca tctgcaaaaa gcaaactact gatttattta
     9181 tcagcggtgg agctttgctt tttttccggc gtgatcgatt tctcctttga gaaattgagg
     9241 acctgctatt acctgaaata aagagatgaa taaaatgtca gaattaaaaa ttgccgttag
     9301 ccgttcttgc ccggattgtt tttccactca tcgtgcatgc gtgaatattg acgaaagtaa
     9361 ttatattgac gttgccgcca ttattttatc agtcaatgat gttgaacgtg gaaaactcga
     9421 tgaaatagac gctactggct atggcattcc tgtttttatt gcaacggaaa atgaagaacg
     9481 tgtaccagca gaatatctgc cacgtatttc tggtgtcttc gagcattgcg aatcacggaa
     9541 agagttttat ggacgtcagt tagaaaccgc tgctagccat tatgaaacgc aactgcgccc
     9601 gcccttcttc cgcgcactgg tcgattatgt caatcagggc aatagcgctt ttgactgccc
     9661 gggtcatcag ggaggtgagt ttttccgtcg tcatccggca gggaatcagt tcgtggagta
     9721 cttcggagaa acgctgttcc gttccgactt atgcaacgca gacgtagcaa tgggcgatct
     9781 gctgattcac gaaggcgcgc catgcattgc acagcaacat gcggcaaaag tgtttaatgc
     9841 cgataaaaca tacttcgttc taaatggcac ttcatcttct aacaaagtgg ttttaaacgc
     9901 cctgctaaca ccgggtgatc tggtgctgtt tgatcgcaat aaccacaaat ctaaccacca
     9961 cggagcgttg ctacaggctg gtgcaacacc ggtttatctg gaaacggcac gtaacccgta
    10021 tggctttatc ggtggcattg atgcgcactg ttttgaagaa agttacctgc gtgagctgat
    10081 cgcggaagtc gcaccgcagc gggcaaaaga ggctcgtcct ttccgcctcg ctgtgattca
    10141 gttaggcacc tacgacggta cgatttataa cgcccgccag gtggtggata aaattggtca
    10201 tctgtgtgac tacattctgt ttgactccgc atgggttggc tatgagcaat ttatcccgat
    10261 gatggctgac tgttcgccgc tgttgctgga tcttaatgag aacgatccgg gtattctggt
    10321 tacgcaatcc gtacataaac agcaggctgg tttttctcaa acttcacaaa tccataaaaa
    10381 agacagccac atcaaagggc aacagcgtta tgtaccgcac aaacgcatga acaacgcctt
    10441 tatgatgcac gcctccacca gcccgttcta tccgctgttt gccgcactgg atatcaacgc
    10501 caaaatgcat gaaggtgtca gcggtcgtaa tatgtggatg gattgtgtgg taaatggcat
    10561 taatgcccgc aaactgatcc tcgataactg tcagcatatt cgtccgttcg tacctgaact
    10621 ggtggatggt aaaccctggc agtcgtatga aacagcgcaa attgcggttg atctgcgctt
    10681 cttccagttt gtaccagggg aacactggca ttcttttgaa ggctatgcag agaatcaata
    10741 ctttgtcgat ccttgcaaac tgttgctgac aaccccaggt attgatgcac gtaacggcga
    10801 atatgaagcg ttcggtgtac ccgcgacgat tcttgctaac ttcctgcgcg aaaatggcgt
    10861 agtgccggaa aaatgcgatc ttaactccat cctcttcctg ctgactccgg cagaagatat
    10921 ggccaaactt cagcaacttg ttgccctgct ggtacgcttc gaaaaactgc ttgagtccga
    10981 cgcgccatta gcagaagtgc tgccttccat ctacaaacag catgaagagc gctacgccgg
    11041 ttataccctg cgtcagttgt gccaggaaat gcatgatttg tatgcccgcc acaacgtgaa
    11101 acaactgcaa aaagagatgt tccgcaaagc gcacttccca cacgtcagca tgaatccgca
    11161 agaagccaac tacgcctatt tacgcggtga agtggagctg gttcgtctgc cggatgccga
    11221 aggtcgtatc gctgccgaag gggcgcttcc ttatcctccg ggtgtgctgt gtgttgttcc
    11281 gggtgaaatc tggggtggtg ctgttttgcg ttacttcagc gctctggaag aagggatcaa
    11341 cctgctgcca ggttttgcac cggagctgca gggtgtctat atcgaagaac atgatggtcg
    11401 taagcaagtt tggtgctatg tcatcaagcc tcgtgatgcg caaagcaccc tgttgaaagg
    11461 ggaaaaatta tgagtcaggc taaatcgaac aaaatgggcg tcgttcagtt aaccatactg
    11521 acgatggtca acatgatggg ctccggtatc atcatgctgc cgacaaagct tgccgaagtt
    11581 gggacaattt caattatctc ctggctggtg acagccgtag gctcaatggc actggcatgg
    11641 gcattcgcaa aatgcggtat gttcagccgt aaatcaggtg gtatgggcgg ttatgccgag
    11701 tatgctttcg gtaaatccgg taactttatg gcgaactata cctacggcgt ctcactgctg
    11761 attgctaacg tcgcgattgc tatttcggcg gttggttacg gcaccgaact gctcggcgcg
    11821 agtttgtcgc cagtgcagat tggtcttgcg accatcgggg tgctgtggat ttgtaccgtg
    11881 gctaactttg gtggtgcgcg cattaccggg caaatcagta gcattaccgt gtggggggtc
    11941 attattccgg tggtaggcct gtgcattatc ggctggttct ggtttagccc gacgctttac
    12001 gttgattcct ggaatccgca tcatgcaccg ttcttcagtg cagtaggttc ttccatcgct
    12061 atgacgctgt gggcttttct cggtctggag tctgcgtgtg cgaatactga tgtagtggaa
    12121 aacccggaac gtaatgtgcc aatcgcggta ctcggcggta cgttaggtgc ggcggtgatt
    12181 tatatcgtct ccaccaacgt gattgcaggg attgtgccta atatggagct ggcaaattca
    12241 acggcaccat ttggcctggc cttcgcacag atgttcacac cagaagtggg taaagtcatt
    12301 atggcgctga tggtgatgtc ctgctgcggt tcgctgcttg gctggcagtt caccattgcc
    12361 caggtgttta agtcttcatc tgatgaaggc tacttcccta aaattttctc ccgtgtaacc
    12421 aaagtggatg caccggtgca gggaatgttg accattgtga ttatccagag cgggttggcg
    12481 ctgatgacga ttagcccgtc gctaaacagt cagttcaacg tgctggttaa cctggccgtg
    12541 gtgaccaata tcattccgta tattctgtca atggcggcat tagtcattat tcagaaggtc
    12601 gctaatgtgc ccccctcaaa agcgaaagtt gcaaactttg ttgcttttgt tggcgcgatg
    12661 tatagctttt atgcgctgta ctcatccggg gaagaagcca tgctgtacgg ttccatcgtg
    12721 accttccttg gttggacact gtatggtctg gtatcaccac gctttgaact gaaaaataaa
    12781 cacggttaaa aaagcaaagt aaaaaagggc gatcttgcga ccgccctttt tttattaaat
    12841 gtgtttacgc gtttttcaga acttcgctaa caatctcaac cgcttctttc tcaatctgct
    12901 tgcgatgttc ttcaccgagg aagctttcgc agtagatctt atatgcgtct tccgtgcctg
    12961 acggacgcgc ggcgaaccag ccgttgtcag tcatcacttt cagaccgccg atagaagcac
    13021 cattgcccgg tgccgccgtc aggcgcgcgg tgatcgggtc gcctgccagg gtgctggcgc
    13081 tcaccatttc cggagacagc ttagacagcg ccgctttttg tgcggaagtc gcagctgcct
    13141 gcaaacggtt gtagctcggc gcaccaaagc gttttgccag ttcgttgtag tgttcctgcg
    13201 ggttcttacc ggtgacagcg gtgatttccg ccgccagcag acacatgatg atgccgtctt
    13261 tgtcggtgga ccacggcgtg ccgtcgaaac gcaggaagga agcccctgcg ctctcttcgc
    13321 cgccaaagcc gaagctgccg tcgaacagac catcaacaaa ccatttgaaa cctaccggga
    13381 cttctaccag cttgcggccc aagtcattga ccacacggtc gatcatcgca gaagaaacca
    13441 gcgttttacc gacagcaaca tctttgcccc actgcggacg atgctggaac aggtagttga
    13501 tcgccaccgc caggtagtgg ttcggattca tcaaacctgc cggagtgacg ataccgtgac
    13561 ggtcataatc cgggtcgtta gcaaacgcca gatcgaactt atcacgcaat gccagcaggc
    13621 ccgccatcgc acactcggag gagcagtcca tacggatcgc gccgtcttta tcgaggtgca
    13681 taaagcggaa ggtttgatcg acctgatcgt taacgatggt caggttgagg ttgtaatact
    13741 cgccaattcg cttccagtat tcgataccgg aaccacccag cggatcaacg cccagcgtca
    13801 ggcccgtttt ctgaatcgcc gccatatcaa cgatatcggc cagaccttcc acaaactgct
    13861 gcaccagatc ctgctctttc acatgaccgg atgccatcgc ttcgtcgagg gagatacgct
    13921 tcacgccttt caggccatcg gccatcagtg cgttggcacg atcttccacc actttagtga
    13981 cgttggtatc agccgggcca ccatttggcg gattgtactt gataccacca tcttccggcg
    14041 ggttatggga gggtgtaatc acgataccgt ctgccagcgg gccacctttt ttattatgaa
    14101 ccaggatggc attggaaacg gcaggcgtcg gagtgaagcc attgttttcc tgcacaatga
    14161 catcaacgcc gttcgctgcc agcacttcca gtacggaaat gaacgcaggc tcggacaggg
    14221 cgtgagtatc tttacccaca tagcaagggc cagtgatgcc gtttttcgca cgttcttcag
    14281 caattgcctg agcgatcgcc agaatgtgcg gctcgttaaa gctgtggcgc gctgcactgc
    14341 cacggtgacc ggaagtaccg aatttcaccg cgtgctccgc attccctgct tctggtttca
    14401 gtacataata ttgcgccgtc agttgggcga cgttaatcaa atcactctgt tgtgcaggtt
    14461 ggcctgcacg attgtggatt gccattgctt tgtcctttgt ctgcaacgtt ttagatagtt
    14521 ccgcaaacct tctcaatcaa ttccgccggg aattgcatcg actgcatgat gtgttcgatc
    14581 atgctgcatt tacggccggt gttggtgttg gtgatcaccc aatacggcgt gcctggcaca
    14641 tgtttcggct tggtctgatt accatttttc agcagcgttt gttcatctgc cgcaaagtaa
    14701 acgcgtgtac gaccgtgcaa cgattccgtt gcttcggcaa acgcctgggc gtcaagagaa
    14761 tatagtgtag acaacagcag cataaagcga ttgaccgctc gcttttgctc tgcgtattcg
    14821 tcagaaagca gaagttcacg cattgcgcga accttgtctt taatcgtttt gaccggcttc
    14881 gcttcgacga tagcaggtga cgcaacgcga acctctttcg tcaccggagc agcaggctgt
    14941 gatgcggcgg aaaatttcaa catacgccgt aaaatgtcgg atgcgctctc gccgatatgc
    15001 ttagtgtggc tggcaatata gctgtagagt tcatcatcaa cttcaatcgt tttcatctta
    15061 atccagtaca gtgtcttagc tgaatacaag tcgccaagag tatatgaata aatcccggca
    15121 gcggatagcg tcaagcctgg cttgcagaaa aactcggaat aaaccttatt tgttgcagcc
    15181 ctgtggcaca atggtcaaca taatacccta acccgacaac gcgaaaaaaa gaactttgcc
    15241 atgaaattga atatccgcgc gcaaactgca caaaaccagc acaataattc tcccatcgtt
    15301 cttgtccatg gtctgtttgg tagcctcgac aaccttggcg tactggctcg cgatctggta
    15361 aacgatcaca atatcatcca ggttgatatg cgtaaccacg gtctttcacc gagagatccg
    15421 gtaatgaatt acccggcgat ggcgcaggat cttgttgata ctctggatgc acagcagatc
    15481 gacaaagcaa catttatcgg tcactccatg ggcggtaaag cggtaatggc acttacggca
    15541 ctggcccgcg atcgcatcga taaactggtg gcgatcgata tcgcaccggt cgactatcac
    15601 gtacgccgtc atgatgagat tttcgcggct atcaacgcgg tcagtgaatc ggacgcacaa
    15661 actcgccagc aagcagcagc aataatgcac cagcatctta atgaagaagg ggtgattcag
    15721 tttctgttga aatcttttgt tgacggggag tggcgcttta acgtgccggt attgtgggat
    15781 cagtatccgc atattgtggg ttgggagaaa atcccggcat gggatcaccc tgccctgttt
    15841 atccctggcg gcaattctcc gtatgttagc gagcagtacc gtgatgattt actggcacaa
    15901 tttccacagg cacgggcgca tgtgattgca ggcgcgggtc actgggtcca tgctgaaaaa
    15961 ccggatgcgg tattacgcgc tatccgtcgc tatctcaatg attaacgcaa ctgattaaaa
    16021 acccagcgtc cgcgtcgctt tccgcgcgtg ggcgttggcg tgactcatcg cgctgatgta
    16081 ttatggcgcg ctatccatcc gggcagaggc ctggctgatt gtttcccccg aagtcaccaa
    16141 gatcatggcc aaagaacaaa cggaccgtac gacattagat ctgttcgcgc acgagcgtcg
    16201 accgggacga ccgaaaacta atccgctttc gcgcgatgaa cagctgcgta ttaataaacg
    16261 caaccagcta aaacgcgaca aagtacgtgg ccttaagcgt gtcgaactga agctgaacgc
    16321 ggaagctgtc gaggcgctga acgagctggc ggagtcgcgc aatatgagcc gtagcgaact
    16381 gatcgaagag atgttaatgc agcaactggc ggcgctgcgt agtcagggca tcgtttaaat
    16441 ttccactttc atgtagcaca gtgtgcagtc ctgctcgttt gctgattccg caagactgcc
    16501 tgttctgcta tgattgcctt tatccgtggg caattttcca cccccatttc aataagtttc
    16561 aagaggttat ttcactcatg gctatcactg gcatcttttt cggcagcgac accggtaata
    16621 ccgaaaatat cgcaaaaatg attcaaaaac agcttggtaa agacgttgcc gatgtccatg
    16681 acattgcaaa aagcagcaaa gaagatctgg aagcttatga cattctgctg ctgggcatcc
    16741 caacctggta ttatggcgaa gcgcagtgtg actgggatga cttcttcccg actctcgaag
    16801 agattgattt caacggcaaa ctggttgcgc tgtttggttg tggtgaccag gaagattacg
    16861 ccgaatattt ctgcgacgca ttgggcacca tccgcgacat cattgaaccg cgcggtgcaa
    16921 cgatcgttgg tcactggcca actgcgggct atcatttcga agcatcaaaa ggtctggctg
    16981 atgacgacca ctttgtcggt ctggctatcg acgaagaccg tcagccggaa ctgaccgctg
    17041 aacgtgtaga aaaatgggtt aaacagattt ctgaagagtt gcatctcgac gaaattctca
    17101 atgcctgatg tgatgcggcg tagactcatg tctacgccgt attaatagat aatgccaatc
    17161 aaaataattg ctacaaattt gtaacttttg ctgttgtacc tgtacaatgt cccggtgttc
    17221 aagtggcctt gccgttgtaa atgtaagctg tgccacgttt ttattaacaa tatttgccag
    17281 ggacttgtgg ttttcattta ggcgtggcaa ttctataatg atacgcatta tctcaagagc
    17341 aaattctgtc acttcttcta atgaagtgaa ccgcttagta acaggacaga ttccgcatga
    17401 ctgataacaa taccgcccta aagaaagctg gcctgaaagt aacgcttcct cgtttaaaaa
    17461 tcctagaagt tcttcaggaa ccggacaacc atcacgtcag tgcggaagat ttatacaaac
    17521 gtctgatcga tatgggtgaa gaaattggtc tggctacggt atatcgcgta ctgaaccagt
    17581 ttgacgacgc tggtatcgtc acccgccaca attttgaagg cggtaaatcc gtatttgaac
    17641 tgacacagca acatcaccac gatcacctga tctgcctcga ctgcggcaag gttatcgaat
    17701 ttagtgatga ttccatcgaa gcgcgtcagc gtgaaattgc cgcaaaacat ggcattcgcc
    17761 tgactaacca cagtctctat ctttacggtc actgtgccga aggcgattgc cgcgaagatg
    17821 aacatgcgca cgaaggcaaa taagccagcc tgaatgagaa aaagccaacc tgcgggttgg
    17881 cttttttatg caagggaaat aagaaaagcg agagttacag ctctcactta tttgttatta
    17941 ctgcgaattt cctgccaaac tttatcacaa tcggctttca ccgcctgatc atttccggtt
    18001 tgcgttgcac gcagatactg ctgataatcc agtacgcgga cactttcctg gtcggcaaaa
    18061 tgctgatgtt gtttctcttt cttcagcacg tttaacacgc tctggcaggc ttcaattttt
    18121 tctggcgaac cttgtgcggt gttgatacag gcgctgtatg cctctttcag acgactgtct
    18181 tcctgtggcg cggttgattg cgcacaagct accagccccg atgccattat ggcgatgaga
    18241 atcagttttt tcatagcaaa ttcctcaaac gtgcggcagg cacaaaacct gccgcgtcgg
    18301 gcatcagaag atggtgaatg gtgcgattac cataaatttc acgtcacgct catcctggaa
    18361 gatgttgccg taaccaccgc cccagcttgg gatgtcggag tggttgtcgt attcggtgaa
    18421 gtgcagtttg aacatcgtgc ctttggcgcg accgtcctga atggtgtaga ccgcatccag
    18481 gctgtatgca gactcttcaa tagtccggtt tttgtcgtag tacgcatccg gattgctctg
    18541 ctaggtcgca ggtttagcat cccatgcgta aacgtaggaa gcgccgatgg cgaagcctgg
    18601 aagattccag tttttcaggt catacatcgc accgaagaag accgcttttt cgccgttagc
    18661 gttgaagtcg gaacggttat cccaccagat atccaggcgg ccgttggagg aagcgtaggt
    18721 tggagtcata cgttgcagga agtatccctg ctgaccgtca gccttaaccc aggtgccttc
    18781 gaggcgcaaa tccactacgt cagccgcccg gtaaccaaag gtcaacgctt gcagccaggc
    18841 ggtgccgtca taaaggtcgt tgacgctgcg atcgtcaact ttatcgcgcg taccatagaa
    18901 ctggtagctg gtggttaacg ggctaccggc gatatcaaat ttgtagctgg ctttggcaaa
    18961 atattgatcg atataccctt ccgcctgacc aaatgccgct tccagtacga agttattttt
    19021 gaagtcgtat ttcgccccaa gggagtgcag ataatcaact ttggtggttt tatcgttctg
    19081 ataaaactca tccatttcca gatgccacgg cgctttgtat tcgttggtcc acatgtagga
    19141 gaaactcaac gcaccagcat cgccgtaatc aaaattcgcc ccggcttccg ccccctgata
    19201 agtacctggc ataaagctcc agtgcggcgc taacagcgtt tgaccagttg gctgaatgta
    19261 acctgccctc gcccaaaccg gaccgtattt aaatttggcc gcagctttat acaggcttat
    19321 accgcttttg tcgccggacc agtcttcgtc ataggcttta ttactttttg aaaacgcgat
    19381 ttcgttcggg tgggagctgt cgccgttttc cgccatttca atcgccgtaa acgcagcaat
    19441 atcaaggccg aacatatcag cagcataacc ggactgaaaa tcgaggttgg cattccaggt
    19501 ggagtgagaa aggttggttt tgtatttgtc gccgtcggta acatctttac ggtcgcgttc
    19561 acgctgccag taatatatac cgccggttaa ggttgaatcg tcgatgaatc cttcggcgcg
    19621 agcctccggc attgccataa agcccgacat tgctgtaaca ccggcgatag ccagcgccag
    19681 cgtactacgt ttgccactaa acgtacgcat gggttaatcc tctttgacgt ataaattgct
    19741 gcaccaaagg tgaacagcga aaaaataaaa attaaaaaag tagcgttgag aaaagtctcg
    19801 ctgactacag gaacagacta tttttcgcga cgcgaattat caatctcttt ctgtaaccag
    19861 aatgtaagaa catgagcaac cgcacatatt ttgatgattt ttgttacaag gcttaagttt
    19921 gcggttgaaa ggcttgcaga agaataaaag cgtgaaattc cagcaagatg aaagcggtta
    19981 cattttattt aattatgttt tgttatttta aatttatttt atggataaat tgcgaagcgg
    20041 aattaattcg cgaagaaaag cagaaaaaac gccgctgaac gcggcgtttt ggattgtttg
    20101 catatttctt gccggatgcg gcgtgaacgc cttaaccggc ctacaaaagc acgcaaactc
    20161 aatatattgc agagatcatg taggcctgat aagcgtagtg catcaggcaa tttagcgttt
    20221 gaaactgttt gtctgttcct tgccggatgc gacgtaaacg ccccatccgg catagcgaaa
    20281 cttaaaatta ctcgcctact ttcgcccagg tatcacgcag cccaacggtg cggttaaata
    20341 ccggtttttc cgccgtagaa tggcggctat cgaggcagaa gtaaccttca cgctcaaact
    20401 ggaatgcttt acccgcaacc gcatctttca gcgacggttc agcaaagccc tgtttgatca
    20461 ccagcgattc cgggttaatc accgacagga aatcatccgc agcaccaggg ttcggcacgc
    20521 tgaacagacg gtcgtacaga cggatttcaa ccggcagcgc atgtgccgcg ctcacccagt
    20581 gaataacacc tttgacttta cgaccatctg ccggatcttt gcttaaggtg tcggcgtcat
    20641 aagtacagaa gatggtggtg atattgcctt cggcatcttt ctccacgcgt tctgccttaa
    20701 tgacgtaagc attacgcaga cgcacttctt tacccagcac cagacgtttg tactgcttgt
    20761 tagcttcttc gcggaaatcg gcgcgatcga tccaaatctc accgctaaac ggcacctggc
    20821 ggctacccat ttccggtttg ttcggatggt tcggcatggt aaccatttcg ccttcgccct
    20881 gatagttttc gataaccagt ttcaccggat cgataaccgc cattgcgcgc ggggcgtttt
    20941 cgttgagatc ttcacggatg caggattcca gcgacgccat ctcaatggtg ttgtcctgct
    21001 tggtcacgcc gatgcgtttg cagaactcac gaatagaagc agcagtgtaa ccacgacgac
    21061 gcagaccgga aatggtcggc atacgcgggt catcccagcc ttcaacgtgc ttgtcggtca
    21121 ccagcaggtt cagcttacgc ttggacatca cagtgtattc cagattcagg cgcgagaatt
    21181 cgtactggcg cgggtgaaca ggaatggtga tgttgtccag cacccagtcg tacaggcgac
    21241 ggttgtcctg gaactcaagc gtacacagag agtgcgtaat accttccagg gcatcgctga
    21301 tgcagtgggt gaagtcgtac atcgggtaga tgcaccactt gttgccagtc tggtggtgtt
    21361 cagcaaattt aatgcggtac agcaccggat cgcgcatcac gataaacggt gaagccatgt
    21421 cgattttcgc acgcaggcag gctttacctt cttcaaaacc accggcacgc attttttcga
    21481 acagcgccag gttctcttca acgctgcggt cgcggtacgg gctgttttta cccggttgcg
    21541 tcagggtgcc gcggtattcg cggatctgtt ccggcgtcag ttcatcaacg tacgccaggc
    21601 ctttattgat cagttcgatc gcataggcgt ggagctgatc aaaataatcg gaggagtaac
    21661 ggacgttacc agaccagtga aaacctaacc actctacgtc gtttttgatc gactcaacat
    21721 actcgatatc ttcttttacc gggttagtgt cgtcgaaacg caggttgcac tggcctttat
    21781 agtcctgggc gatcccgaag ttcaggcaga tagatttcgc atggccaata tgcagatagc
    21841 cattcggctc cggcgggaaa cgggtgtgta ctgtggtgtg cttaccactg gccagatctt
    21901 catcgatgat ctgacggata aagttagtcg ggcgggcttc tgcctcactc atcgtggatt
    21961 cctcaaagcg taaacaacgt ataacggcgt atgatcttat aagcgggaca ggctgacaac
    22021 tgttagttag cgaaaatcga aaatctcact gagattatgg ggatgattga tgaaaaaaaa
    22081 agcggcagag aagatctcca ccgcttttgc cagtcccgcg tcattgttgg atgcgcgtct
    22141 tatccaacca ggactgagct tttgtaggcc tgataagacg cgtcaagcgt cgcatcaggc
    22201 ataaagcaga ttactttttg atttcataca gcggtgtttg acccgccaca acattgccct
    22261 gagctttaat gatcaagcca ctgaaatcgt cgatattgct gcaaaccacc gggctaatca
    22321 tcgaacgggc gttagcgttc aggtaatcca gatccatttc cagaatcggt tgccctgcgc
    22381 tcacctgcgc gccctcttcc accagacgtt taaagccttt accttccagc gctacggtgt
    22441 cgatacccat gtggacgaca atctccgcgc ctttttcggt ttctaggcag aacgcgtggt
    22501 tggtgttgaa gattttcacg attgtccctg cggctggtga tacgacgatt ttatctgtcg
    22561 gtttcaccgc cacaccgtca cccaccgctt tgctggcgaa tgcttcgtca gaaacctgat
    22621 ccagtgccac gacatcaccg gtaatcggcg ataccagctc cgcgatagat accgcgtttg
    22681 gtacagcctg cggttttgct acaggagcgg cagttgccgg agttgcttca gctgacgcag
    22741 ctgctaccgg gccacgggca acgactttct tcatcgcatc gccaatggat tctgctttcg
    22801 cgccaacaat cacctgaatg gtctgtttgt tcagttttac tacaccagaa gcacccagac
    22861 gtttacacat cgcatcgttg acgcgagctg agtcagccac ggtaaggcgc agacgggtaa
    22921 tacaggcgtc aatcgctttc aggttgtcag taccgccaac cgcagcaata tagttggttg
    22981 ccagttgagt cagaccttct tcagtattgc tgttagcctc ttcagttacg atctcgtctt
    23041 ctttatcttc acgacccggc gttttcaggt tgaacatgcg gataaccaaa ctgaacacca
    23101 cgaagtagat agcgaagaag acaacaccca tcaccagcag catccagacg ttctggctgg
    23161 cggccggcag gttatacatc aacgcgtagt cgatagcccc cgcagagaaa gagaagccag
    23221 catggatacc cagcagcgtt gccacaaaca ggctgatacc ggtcagcagt gcgtgcagga
    23281 ggtacagcag cggagcaagg aacatgaaca ggaattccag cggctcagtc acaccggtca
    23341 ggaacgcagt aacagcaaca gaaagcagca tcccgccaac catcggacga cgctctttcg
    23401 gtgctgcgaa gtacatcgcc agcgccgcac ccggcagacc gaacatcatg atcgggaaga
    23461 agccggacat gaacatcccc gcggtgccgt caccagcgta gaaacggttg atgtcgccgt
    23521 ggaaaaccgt acccgccgcg ttggtgaatt caccaatctg gaaccaggcg atggtgttca
    23581 gcacctgatg cagaccggtt gggatcagca gacggttgat gaaaccaaag ataccggaac
    23641 ccagcgcgcc tgcagaaacg atccactcgc cgcctgcatg gatagcgtgc tgtactggcg
    23701 gccagacgta accaaaaatg gccgccagca ccaggcagaa aaagccggtg gcaatcggca
    23761 caaagcgttt gccgccgaag aagctcagga agtccggcag tttaatatcg gaccaacggt
    23821 tataggctgc gccaccaacc agaccggtaa tgatacccgc cagtacaccc atgttaattt
    23881 ctgggttgat ggtcaccatc gctttggtta acacaaagta acctaccgca cccgccagcg
    23941 ccgccgcacc tgcgctgtct ttcgaccagc tggatgccac accgatggcg aagattaatg
    24001 cgaggttatc aaaaatcgca ccgcccgcct gggcaataaa cgcaacgtta agtaaatctg
    24061 gctgaccgaa tcgcagcaac agtgccgcca ccggcagcac cgcgataggg agctgtaacg
    24121 ccctaccgag tcgctggaaa aaacctaaaa tattcatctt attcccccta cgagaaccct
    24181 atttggctcg tttaaagccg tatttttatt ttgctgcaaa ttgtactgcc gatgttctgt
    24241 aatcagattg ttagatcatc tgctacagag tgtgtgaaaa tttaattcgt atcgcaaatt
    24301 aaacgcgtgc cttttgtgag ttttgtcacc aaatatcgtt atcatcactc ccttttactg
    24361 gctaaagcag aaaacttatt ttatcattca aaaaatcagg tcggattgac gcctgtctgc
    24421 gcaaatccag gttacgctta aagatgccta atccgccaac ggcttacatt ttacttattg
    24481 aggtgaataa tgagactgat ccccctaact accgctgaac aggtcggcaa atgggctgct
    24541 cgccatatcg tcaatcgtat caatgcgttc aaaccgactg ccgatcgtcc gtttgttctg
    24601 ggcctgccga ctggcggcac gccgatgacc acctataaag cgttagtcga aatgcataaa
    24661 gcaggccagg tcagctttaa gcacgttgtc accttcaaca tggacgaata tgtcggtctg
    24721 ccgaaagagc atccggaaag ctactacagc tttatgcacc gtaatttctt cgatcacgtt
    24781 gatattccag cagaaaacat caaccttctc aacggcaacg ccccggatat cgacgccgaa
    24841 tgccgccagt atgaagaaaa aatccgttct tacggaaaaa ttcatctgtt tatgggcggt
    24901 gtaggtaacg acggtcatat tgcatttaac gaaccggcgt cttctctggc ttctcgtact
    24961 cgtatcaaaa ccctgactca tgacactcgc gtcgcgaact ctcgtttctt tgataacgat
    25021 gtcaatcagg tgccaaaata tgccctgact gtcggtgttg gtacattgct ggatgccgaa
    25081 gaagtgatga ttctggtgct gggtagccag aaagcactgg cactgcaggc tgcagttgaa
    25141 ggttgcgtga accacatgtg gaccatcagc tgtctgcaac tgcatccaaa agcgatcatg
    25201 gtgtgcgatg aaccttccac catggagctg aaagttaaga ctttaagata tttcaatgaa
    25261 ttagaagcag aaaatatcaa aggtctgtaa ttgttatccc tgccctggct ccttgctcag
    25321 ggcaatattt tttaaaatcg gggggcagaa tgtatgcatt aacccagggc cggatcttta
    25381 ccggccacga atttcttgat gaccacgcgg ttgttatcgc tgatggcctg attaaaagcg
    25441 tctgtccggt agcggaactg ccgccagaga tcgaacaacg ttcactgaac ggggccattc
    25501 tctcccccgg ttttatcgat gtgcagttaa acggctgcgg cggcgtacag tttaacgaca
    25561 ccgctgaagc ggtcagcgtg gaaacgctgg aaatcatgca gaaagccaat gagaaatcag
    25621 gctgtactaa ctatctgccg acgcttatca ccaccagcga tgagctgatg aaacagggcg
    25681 tgcgcgttat gcgcgagtac ctggcaagac atccgaatca ggcgttaggt ctgcatctgg
    25741 aaggtccgtg gctgaatctg gtaaaaaaag gcacccataa tccgaatttt gtgcgtaagc
    25801 ctgatgccgc gctggtcgat ttcctgtgtg aaaacgccga cgtcattacc aaagtgaccc
    25861 tggcaccgga aatggttcct gcggaagtca tcagcaaact ggcaaatgcc gggattgtgg
    25921 tttctgccgg tcactccaac gcgacgttga aagaagcgaa agccggtttc cgcgcgggga
    25981 ttacctttgc cacccatctg tacaacgcga tgccgtatat taccggtcgt gaaccgggcc
    26041 tggcgggcgc gatcctcgac gaagctgaca tttattgcgg tattatcgct gatggcctgc
    26101 atgttgatta cgccaacatt cgtaacgcta aacgccttaa aggcgacaaa ctgtgtctgg
    26161 ttaccgacgc caccgcgcca gcaggtgcca acattgaaca gttcattttt gcgggtaaaa
    26221 caatatacta ccgtaacgga ctttgtgtgg atgagaacgg tacgttaagc ggttcatcct
    26281 taaccatgat tgaaggcgtg cgtaatctgg tcgaacattg cggtatcgca ctggatgaag
    26341 tgctgcgtat ggcgacgctc tatccggcgc gtgcgattgg cgttgagaaa cgtctcggca
    26401 cactcgccgc aggtaaagta gccaacctga cctcattcac acctgatttt aaaatcacca
    26461 agaccatcgt taacggtaac gaggtcgtaa ctcaataaga gaaagtatga caccaggcgg
    26521 acaagctcag ataggtaatg ttgatctcgt aaaacagctt aacagcgcgg cggtttatcg
    26581 cctgattgac cagtacgggc caatctcgcg gattcagatt gccgagcaaa gccagcttgc
    26641 ccccgccagc gtaaccaaaa ttacgcgtca gcttatcgaa cgcgggctga tcaaagaagt
    26701 tgatcagcag gcctccaccg ggggccgccg cgctatctcc atcgtcaccg aaacccgcaa
    26761 tttccacgca atcggcgtac ggcttggtcg tcacgacgcc accatcaccc tgtttgatct
    26821 cagcagcaaa gtgctggcag aagaacatta cccgctgccg gaacgtaccc agcaaacgct
    26881 ggaacatgcc ctgttgaatg ccattgctca gtttattgat agctaccagc gcaaactgcg
    26941 cgagctgatc gcgatttcgg tgatcctgcc agggcttgtt gacccggaca gcggcaaaat
    27001 tcattacatg ccgcatattc aggtagaaaa ctgggggctg gtagaagctc tggaagaacg
    27061 ttttaaagtg acctgtttcg ttggtcacga tatccgtagt ctggcgctgg cggagcacta
    27121 cttcggtgca agtcaggatt gcgaagactc cattctggtg cgtgtccatc gcggaaccgg
    27181 ggccgggatt atctctaacg ggcgcatttt tattggccgc aacggtaacg tcggtgaaat
    27241 tggccatatt caggtcgaac cgctgggtga acgctgccac tgcggcaact ttggctgcct
    27301 ggaaactatc gccgccaacg ctgccattga acaacgggtg ttgaatctgt taaagcaggg
    27361 ctaccagagc cgcgtgccgc tggacgactg caccatcaaa actatctgca aagccgcgaa
    27421 caaaggcgat agtctggcgt cggaagtaat tgagtatgtc ggtcgtcatc tgggtaaaac
    27481 catcgccatt gctatcaact tatttaatcc gcaaaaaatt gttattgccg gtgaaatcac
    27541 cgaagccgat aaagtgctgc tccctgctat tgaaagctgc attaataccc aggcgctgaa
    27601 ggcgtttcgt actaatctgc cggtggtacg ttctgagctg gatcaccgct cggcaatcgg
    27661 cgcttttgcg ctggtaaaac gcgccatgct caacggtatt ttgctccagc atttgctgga
    27721 aaattaatgt gcttttatag tggcgcttat tgttgtcaat attctgggta gtccatgacc
    27781 attaaaaatg taatttgcga tatcgacggc gtgctgatgc acgataacgt cgccgtaccg
    27841 ggtgcagcgg aatttttgca cgggattatg gataaaggcc tgccgctggt gttgctgacc
    27901 aactatcctt cgcagaccgg gcaagatctg gcgaaccgct ttgccactgc cggtgtcgat
    27961 gtgccggata gcgtgtttta tacctctgcg atggcgaccg ccgatttcct gcgtcgccag
    28021 gaaggcaaga aagcatatgt ggtgggcgaa ggcgcactga ttcatgaact gtacaaagcc
    28081 ggtttcacta ttaccgatgt gaaccctgat ttcgtgattg ttggcgaaac gcgttcctac
    28141 aactgggaca tgatgcataa agcggcctat ttcgtcgcta acggtgcacg ctttatcgcc
    28201 accaacccgg acacccacgg gcgcggtttt tatcccgctt gtggcgcgtt gtgtgcaggg
    28261 attgagaaaa tctccgggcg caaaccgttc tatgttggta agcccagccc gtggatcatc
    28321 cgcgcagcat taaacaaaat gcaggcgcat tcggaagaaa cggtgattgt cggcgataac
    28381 ttgcgcaccg acattctggc gggcttccag gcaggtctgg aaacgattct ggtgctttct
    28441 ggtgtttcat cgctcgacga tatcgacagt atgccgttcc gcccaagctg gatttacccg
    28501 tcagtcgctg aaatcgacgt tatctgaata ccacccacgt ctgcggacgt ggttggctct
    28561 gatgacaaac gccaaactgc ctgatgcgct acgcttatca ggcctacgca gctcctgcaa
    28621 tatattgaat tttcatgctt ttgtaggccg gataaggcgt tcacgccgca tccggcatga
    28681 acaaatcgca ctttatcagc aatttggcc
//
LOCUS       DAJTZL010000036        28678 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.036, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000036 DAJTZL010000000
VERSION     DAJTZL010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28678
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.036"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..1046)
                     /locus_tag="OO544_001384"
     CDS             complement(<1..1046)
                     /locus_tag="OO544_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HCR6767019.1"
                     /translation="MREINMLRNISSCLFPHISTITSPNHYLSEWDDWEKQGLPEEQR
                     TEAVRRLRACLTSKGHKLDLRALALSSLPVLPACIKKLDVSCNKLTILTDLPENIKEL
                     IARDNFLTHISALPHYLITLDVSENQLENLPLLPDTIKSLSAEYNRLSTLPSLPLNLK
                     KLEVRNNELQTLPSLPSNLKILKVAHNHLTELPPLPRRLQLLFAYSNRLSNLPNIQEN
                     IIMRRFFYFENNQITTIPTNLFRLDPHITIEIANNPLSDQTLLFLIQQTSVPNFNGPQ
                     FRISLSDQNRLFLRQMLPQNLHSRHIRVITEGGQNFQIPPLPETVAAWFPEADRREVS
                     TQWTSFSTEENSRA"
     gene            1537..2094
                     /gene="pinE"
                     /locus_tag="OO544_001385"
                     /pseudo
     CDS             1537..2094
                     /gene="pinE"
                     /locus_tag="OO544_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364430.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA recombinase"
     gene            complement(2433..3281)
                     /locus_tag="OO544_001386"
     CDS             complement(2433..3281)
                     /locus_tag="OO544_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCR6767020.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            3490..4125
                     /gene="yfhb"
                     /locus_tag="OO544_001387"
     CDS             3490..4125
                     /gene="yfhb"
                     /locus_tag="OO544_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HCR6767021.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            4150..4686
                     /gene="tadA"
                     /locus_tag="OO544_001388"
     CDS             4150..4686
                     /gene="tadA"
                     /locus_tag="OO544_001388"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HCR6767022.1"
                     /translation="MRRAFITGVFFLSEVEFSHEYWMRHALTLAKRAWDEREVPVGAV
                     LVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCA
                     GAMIHSRIGRVVFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFF
                     RMRRQEIKAQKKAQPSTD"
     gene            complement(4683..6239)
                     /gene="mltF"
                     /locus_tag="OO544_001389"
     CDS             complement(4683..6239)
                     /gene="mltF"
                     /locus_tag="OO544_001389"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HCR6767023.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVSQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKDTKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQCLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            6497..10384
                     /gene="purL"
                     /locus_tag="OO544_001390"
                     /gene_synonym="purI"
     CDS             6497..10384
                     /gene="purL"
                     /locus_tag="OO544_001390"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HCR6767024.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFALDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFKMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNTLLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGINADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            10942..12369
                     /gene="qseE"
                     /locus_tag="OO544_001391"
     CDS             10942..12369
                     /gene="qseE"
                     /locus_tag="OO544_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HCR6767025.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSS
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            12534..13247
                     /gene="qseG"
                     /locus_tag="OO544_001392"
     CDS             12534..13247
                     /gene="qseG"
                     /locus_tag="OO544_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HCR6767026.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPTGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            13237..14571
                     /gene="glrR"
                     /locus_tag="OO544_001393"
     CDS             13237..14571
                     /gene="glrR"
                     /locus_tag="OO544_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HCR6767027.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            14632..14970
                     /gene="glnB"
                     /locus_tag="OO544_001394"
     CDS             14632..14970
                     /gene="glnB"
                     /locus_tag="OO544_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HCR6767028.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(15015..16205)
                     /gene="hmpA"
                     /locus_tag="OO544_001395"
     CDS             complement(15015..16205)
                     /gene="hmpA"
                     /locus_tag="OO544_001395"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HCR6767029.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWHRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            16533..17786
                     /gene="glyA"
                     /locus_tag="OO544_001396"
     CDS             16533..17786
                     /gene="glyA"
                     /locus_tag="OO544_001396"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HCR6767030.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARFPVYA"
     gene            complement(17984..19177)
                     /locus_tag="OO544_001397"
                     /pseudo
     CDS             complement(17984..19177)
                     /locus_tag="OO544_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311443.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
     gene            19295..22572
                     /locus_tag="OO544_001398"
                     /pseudo
     CDS             19295..22572
                     /locus_tag="OO544_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
     gene            22669..23652
                     /locus_tag="OO544_001399"
     CDS             22669..23652
                     /locus_tag="OO544_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCR6767031.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDTLGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYISAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLNKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            23675..25186
                     /locus_tag="OO544_001400"
     CDS             23675..25186
                     /locus_tag="OO544_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCR6767032.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKITRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            25211..26209
                     /locus_tag="OO544_001401"
     CDS             25211..26209
                     /locus_tag="OO544_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6767033.1"
                     /translation="MSASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            26242..27336
                     /locus_tag="OO544_001402"
     CDS             26242..27336
                     /locus_tag="OO544_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCR6767034.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGVMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYALMASGKCGKVVINFPD"
     gene            27348..28220
                     /locus_tag="OO544_001403"
     CDS             27348..28220
                     /locus_tag="OO544_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HCR6767035.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(28268..>28678)
                     /locus_tag="OO544_001404"
     CDS             complement(28268..>28678)
                     /locus_tag="OO544_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCR6767036.1"
                     /translation="RYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQYMAS
                     LGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDMTGDA
                     VGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
BASE COUNT         6818 a         7092 c         7790 g         6978 t
ORIGIN      
        1 aatgcccggg aattctcctc ggtggaaaaa gaagtccatt gtgtagaaac ctcccgacga
       61 tctgcttcag gaaaccaggc tgccacagtt tcgggaagag gggggatctg aaagttctgc
      121 cccccttcag tgatgactct gatatggcgc gaatgtaaat tttgcggcaa catctggcgt
      181 aaaaacagtc tgttttggtc tgacagggaa atacgaaact gaggcccgtt aaaatttgga
      241 accgaagttt gctgtattaa gaatagcaga gtttgatctg ataaggggtt atttgcaatc
      301 tcaatagtta tatgaggatc taaacgaaaa agatttgtcg ggattgtagt tatttggttg
      361 ttttcaaaat aaaaaaatct tctcatgata atattttctt ggatgtttgg taagttgctt
      421 aatctattgc tatatgcaaa aagaagttgc agtctcctag gtaaaggggg cagttcagta
      481 agatggttgt gcgcaacctt aagtatctta agattagaag gcagagatgg aagagtttgc
      541 agttcgttgt tcctaacctc aagttttttt aaattcaagg gtaatgaagg cagtgtggat
      601 aacctattat actctgcgct tagtgatttg atggtgtctg gtaataacgg cagattctct
      661 aattgatttt cggacacatc caaagttatt agataatgtg gtaatgcaga tatatgtgtt
      721 aagaaattat ctcttgcaat aagttcttta atattttcag gtaaatcagt aaggatggtt
      781 aatttattac agctcacatc aagcttttta atgcaagcag ggagtacagg taacgaggaa
      841 agcgccaagg ctcgcaggtc cagtttatgc cccttagagg taagacatgc acgaagtctt
      901 cttaccgcct cagtacgctg ttcttccggt aacccctgtt tctcccaatc atcccattcg
      961 gacaaataat ggttggggga tgtaattgtg ctgatatgtg gaaataaaca ggatgaaata
     1021 tttctgagca tattaatttc tcgcatatgg atatggaatt agattgtgaa taaattgtat
     1081 ttaatgtatc tctcctaatg aggagggtga ttcagaatat tcgccataag ggaatgagtg
     1141 ttatatcatg ctttctaccc tggtaagtat atttgctttt ttcgtatatt tggttcatct
     1201 ttttttgatg ttttacatat tcacaatgca gaggatttat accagaagac attagtcttg
     1261 agtaagatac tacaacatct ccaactattt tagccagcct gactacttga cttggtaata
     1321 tttgttgcta ataatcatgt ataaaataag cttatataca tagtaacttc agcaaaggat
     1381 ttagtaatga tccaaatcat atctcaatgt actgtttttt tctttaaaaa aacagtacat
     1441 tctactttat aatgactgtg tattcccatg gaaggagatc acccaattct cccccccgac
     1501 ttttgacgca gtgtcttgtt aacaggggga tttaccatgc ttattggcta tgtacgcgta
     1561 tcaacaaatg accagaatac cgatctacaa cgtaatgcgt tgaactgtgc aggatgcgag
     1621 ctgatttttg aagacaagat aagtggcaca aagtccgaaa ggccggggct gaaaaaactg
     1681 ctcaggacat tatcggcagg cgacacactg gttgtctgga agctggatcg gctggggcgc
     1741 agtatgcggc atcttgtcgt gctggtggag gagttgcgcg aacgaggcat caactttcgt
     1801 agtctgactg attcaattga taccagcaca ccaatgggac gctttttctt ccatgtgatg
     1861 ggtgccctgg ctgaaatgga acgtgaactg attgttgaac gaacaaaagc aggactggaa
     1921 gctgctcgcg catagggacg aattggcggt cgccgtccca aactttcacc agaataatgg
     1981 gcgcaggccg ggcgactaat tgcatcagga gtttctcgcc agaaggtggc gatcatctat
     2041 gatgttggcg aatcgacttt tttgtataag aggtttccag ccagggttat ataaaaccgc
     2101 agacacggcg tatacaagaa cgtgctacgg ttgactgaaa agttaatgtg gtatgtcaca
     2161 ggccttttgc gccgtaaagc cccgtccttt agggcgcgga ggacgttaaa gaaagtggtg
     2221 ttctttgtgt tgctgaactt atgcaaaatt tttttgcacc ataaatccaa caacacattc
     2281 aataacacat tgctgtatca ctatgaaaat aaatgtgttt ttatttgatt tcacacatat
     2341 cacaattgat gcatttttta gtgattaaca acgccataag gaaactctca aaacaacaca
     2401 aaccgggcgg gcattgtacg cgcattcttt actcagacca gttttttcac cagcgcttca
     2461 ctatgacgaa tacgttctgg tgccagttcc agatcctgct gaatcagcgc aatgaacagc
     2521 aaatccgtta acattccctg agcgtgacaa gcagagattg aagcactgtt tgtcgcttgt
     2581 tcttcggcaa tggtatacag gcaatgagaa gcacgctgtt gcagggcatt cggagtaaag
     2641 ccggtaatcg ccagcacttt tccgcccact cgcagcatct catctgccgc caggtttaac
     2701 tcgcgtcgta caccggtgta ggaaatggct aataacagat cgtcagggga cgacgcctgt
     2761 actgttgcga gtagcgcatg catatcgcgc actgcggcag cattgaagcc aatcttcatc
     2821 agcttccagg caaagttttg cgccaccaga cccgaagcgc caataccggt cagaattatc
     2881 cgccgcgcag agcgcaacat tgttacgcat tcatgcagtt tctcttcact attaacgttt
     2941 agcgttgcgt acatagcggc agtattttct ttaatcagtt tttcgccgac cagccgtaac
     3001 ggatcatcac cgcggatttg gttatgaatg ggcacggagg gcgattccgg ctggcttgcc
     3061 agcgcttcac tcaacgccaa cttaagcgcc ggaaaacctt tatagccgag tttttgcgcg
     3121 aacttcacga cgctggactg actgactccg gcttcgttgg ccagttgctg agagcttaaa
     3181 tggcgcgccg tatcaggttg tagcagcaga taatccgcca gttttttatc actctgggca
     3241 agcccctggt aacgctgacg gatacgaagt aagccgttca tatctttcct cgcgatgaaa
     3301 tagtggcgac aacaggcaat atgagacaca cgcgagtgaa ttttatattc cattgccgct
     3361 aattgccatg aattaaaaat tccagttagc catgatgcgg tcgattgttg ccggaaaagt
     3421 gatgatctat ctgttaggct atgtagtagc gaaaattaat caggatattt cagtccagag
     3481 gagtatggtt tggcaactca cgagcgtcgt gtggtgtttt ttgacttaga tggaacattg
     3541 catcagcagg atatgttcgg cagttttctg cgctatttac tgcgtcgcca accgctgaat
     3601 gcgttacttg tcctgccgtt gttaccgatt atagccattg cgttattgat aaaaggtcgt
     3661 gcggcacgct ggccgatgag tctgcttctg tgggggtgca cttttggtca cagcgaagca
     3721 cgtttacaga cgttgcaggc cgatttcgtg cgctggtttc gcgacaatgt taccgccttt
     3781 ccgctggttc aggagcgatt aaccacctac ctgttaagtt ccgatgctga tatctggttg
     3841 attaccggct ctccgcagcc gctggttgaa gcggtttatt tcgatacgcc ctggctgccg
     3901 cgggttaatc ttatcgccag ccaaattcag cgtggctatg gtggttgggt attgacgatg
     3961 cgttgtctgg gacatgaaaa ggtcgcacaa ctggagcgca aaatcggcac tccgctgcgg
     4021 ctgtacagtg gctatagcga cagtaatcag gacaatccgc tgctttattt ctgtcagcat
     4081 cgttggcgag taaccccgcg cggtgaactc cagcaactgg aatagagtaa agcatagcgt
     4141 ccgtgtataa tgcgccgcgc ttttataacc ggagttttct ttttgtctga agtcgaattt
     4201 agccacgaat actggatgcg tcacgcgctg acgctggcga aacgtgcctg ggatgagcgg
     4261 gaagtgccgg tcggcgcggt attagtgcat aacaatcggg taatcggcga aggctggaac
     4321 cgcccgattg gtcgccatga tcccaccgca catgcagaaa tcatggccct gcggcagggt
     4381 ggtctggtga tgcaaaatta tcgtctgatc gacgccacgt tgtatgtcac gcttgaacca
     4441 tgtgtaatgt gtgccggagc gatgatccac agtcgcattg gtcgcgtggt ctttggtgcg
     4501 cgtgacgcga aaactggcgc tgcgggatct ttaatggatg tgctgcatca tccgggtatg
     4561 aatcaccgag tggaaattac ggaaggaata ctggcggatg agtgcgcggc gttgctcagt
     4621 gacttctttc gcatgcgccg ccaggaaatt aaagcgcaga aaaaagcgca accctcgacg
     4681 gattaatttt gtttctcttc actccctttc ctggaaaaca gcagagaggg agaatgggtc
     4741 aaatagtttg atgacgactg ggaaagaaac gagagaaaag gaaatttctc tttgcccaac
     4801 tccgtaggcg ataccgccgg ataatcctgc gccagttgca tcgccgcttc tgtagcctgc
     4861 ttctctttct cttgcagata acccaccagg ctaatctgat acttacgaat attttcgaca
     4921 taagcgtagg cttcatgtcc acgagcgtag ccgtaagtca gcttgctgta atagggtttc
     4981 tggctaagta aaggcagaca ctgttttacg tcagcccaac tgtcaggatt ccctttggtt
     5041 tttgccgtca gggcgcgggc atccagcata tgcgcatagc ccatattgta cgcagcgagg
     5101 gcaaaccaga tccgctcgtt ctccggcaca ctttccggca ctttactcat catatcctgc
     5161 aaataacgca cgccaccgct gatgctctgt tcggcatcgg tacgatccgt aatgccgagg
     5221 ctttgcgcgg tatttttggt taacatcatc atgccgcgca cacccgtcgg tgaagtggcc
     5281 tgtgcatccc agtgcgattc ctgataagca atagcggcca gcaaacgcca gtcaatttct
     5341 tcggcgtatt tctcaaacag gggctttaac tgcggcagta ccgcatcgac ggcgcgtaaa
     5401 aatgtgcgcg tatcgacgta atcaaaatca tcgccatgcc ccaggtattt ctcttcaatg
     5461 cgtgccagcg taccgtcttc attcatttcg ttgaagaagt cgagcagggc ggcggaaagg
     5521 gtattatcgc catctaacgg gctaaaccaa gtcaccggtt gttcatcggt gatatcgagc
     5581 gctacggcga gctccgggtg aacgcgctga aacaggctga tggcgacaga atcagcaatg
     5641 gtgtaatcga gttttccttc gatgacatct tccattaatt ccgcagagcc ttttttgtcg
     5701 tctaccttcc agcttaattc cgggaatttt gtgtctttca gggtttggag atcgttaacc
     5761 accacgtgac ccggtgcaac ggtgagttgc tccgccgtca ggttgcccag tgtgcgcggg
     5821 cgatactgac tcactttata aaccagttgt tgtgacacgg aataataggt agggccaggc
     5881 tgataatttt ttacccgctc actgttatag accagtcctg ccgccagcag gtcggcgtta
     5941 ccattatcga ggtcgtcaaa cagctggctg atattctgcc gcacggtcac tttcagtttt
     6001 acaccgaggt aatcggcaaa ctgtttcgcc agttcgtaat ccaggccaaa aggtttcccg
     6061 ttgatttcgt tataagtcag gggagtatga atggtgctca cacgcaactc tccccgcgct
     6121 tgaatggcgg cgatacggtt gtcggcttta ccaaaccagg gaatggatgg ccagagagcg
     6181 accgcgagca gcagtgccag aatgccgatg aacagataat taatctttaa ttttttcaat
     6241 tagttaattc tctgtgtcgt gcgcgtccca gcttgaaaaa acgtaataat agtgaaaggt
     6301 ttactcataa atgagcggca ttttgcgtaa acctgcgcca gatggcaact tattacagcc
     6361 attggcggca cgcgttgcta attcacgatg gtgattttat ttccacgcaa acggtttcgt
     6421 cagcgcatca gattctttat aatgacgccc gtttcccccc cttgggtaca ccgaaagctt
     6481 agaagacgag agacttatga tggaaattct gcgtggttcg cctgcactgt cggcattccg
     6541 aatcaacaaa ctgctggcac gttttcaggc tgccaggctc ccggttcaca atatttacgc
     6601 cgagtatgtc cattttgctg acctcaatgc gccgttaaac gatgatgagc acgcacaact
     6661 tgaacgcctg ctgaaatatg gcccggcact cgccagccac gccccgcaag gcaaactcct
     6721 gctggtgacc ccgcgtcctg gcaccatctc tccctggtct tcgaaagcga ccgatattgc
     6781 ccataactgc gggctacaac aggtaaaccg ccttgagcgc ggcgttgctt actatataga
     6841 agccggtacg ctgaccaatg aacaatggca gcaggttacc gctgaactgc acgaccgcat
     6901 gatggaaacg gtcttttttg ctttagatga tgcagagcag ttgtttgccc accatcaacc
     6961 gactccggtt accagcgttg atttgctggg gcagggccgt caggcgctga tcgacgctaa
     7021 cctgcgtctt ggcctggctc tggcggaaga tgaaattgac tatctgcagg atgctttcac
     7081 aaagcttggt cgtaacccga acgacatcga gctgtatatg ttcgctcagg cgaactctga
     7141 gcactgccgc cacaaaattt ttaatgccga ctggattatc gacggtgaac agcagccgaa
     7201 atcgctgttc aagatgatca aaaatacttt cgaaaccacg ccagatcacg ttctctctgc
     7261 ttataaagat aacgccgccg taatggaagg ttctgaagtg ggccgctact ttgctgacca
     7321 cgaaacgggc cgctacgatt tccatcagga accggcgcat attctgatga aagtcgaaac
     7381 tcacaaccac ccgacggcga tttctccgtg gccgggcgcg gcgacgggtt ccggcggcga
     7441 aattcgtgat gaaggcgcca ccggacgcgg tgcgaagccg aaggctggtc tggttggttt
     7501 ctccgtttcc aacctgcgaa ttcctggctt cgaacagccg tgggaagaag atttcggtaa
     7561 gcctgagcgc attgtcaccg cgctggacat catgaccgaa ggcccgctgg gcggcgcggc
     7621 gtttaacaac gaatttggtc gtccggcact gaatggctac ttccgtactt atgaagaaaa
     7681 agtgaacagc cacaacggcg aagagctgcg cggttatcac aaaccgatca tgctggcggg
     7741 cgggatcggc aacattcgcg ccgatcacgt acaaaaaggc gagatcaacg tcggtgcgaa
     7801 gctggtcgtt ctcggcggcc cggcaatgaa catcggtctt ggcggcggag cggcgtcttc
     7861 catggcgtca ggtcagtctg atgccgacct cgactttgct tccgtacagc gcgacaaccc
     7921 ggagatggag cgtcgctgcc aggaagtgat cgaccgttgc tggcagcttg gtgatgccaa
     7981 cccaatcctg tttatccacg acgtcggcgc tggcggtctt tctaacgcga tgccggaact
     8041 ggtgagcgac ggcgggcgcg gcggtaaatt tgaactgcgc gatattctaa gcgacgaacc
     8101 gggcatgagc ccgctggaaa tctggtgtaa cgaatcccag gaacgctacg tgctggcggt
     8161 tgctgccgat caattaccgc tgtttgacga actgtgtaag cgtgagcgcg caccctacgc
     8221 ggtgattggt gaagcgaccg aagaactgca tctttctctg cacgatcgtc attttgataa
     8281 tcagccgatc gatctgccgc tggacgtcct gcttggtaaa acgccgaaga tgacccgcga
     8341 tgtacaaacg ctgaaagcga agggcgatgc gctggtccgt gaagggatca ccattgcaga
     8401 cgcggtgaaa cgcgtactgc atctgccgac cgtggcagag aaaaccttcc tggtgaccat
     8461 cggcgaccgc agcgtaaccg gcatggtagc gcgcgatcag atggtggggc cgtggcaggt
     8521 gccggtcgct aactgcgcgg tcactaccgc cagcctcgac agctactacg gtgaagcgat
     8581 ggcgattggc gagcgtgcgc cggttgcact gctggatttc gccgcctctg cccgtctggc
     8641 ggtcggtgaa gcgttaacca atattgccgc aacacagatt ggcgatatca aacgcattaa
     8701 actttccgcc aactggatgg cggcggcagg ccaccctggt gaagatgcgg gcctgtatga
     8761 agccgttaaa gccgtgggcg aagagctttg tccggcgctg ggcctgacga tcccggtggg
     8821 taaagactct atgtcgatga aaacccgctg gcaggaaggt aacgaagagc gcgaaatgac
     8881 gtcgccgctg tcgctggtga tttctgcatt tgcccgcgtg gaagatgtgc gtcacaccat
     8941 cacgccacag ctttctaccg aagataacac actgctgctg attgatttgg gcaaaggcaa
     9001 taacgcgctg ggcgcaacgg cgctggcgca ggtttatcgt cagcttggcg acaaaccggc
     9061 agatgtacgc gacgtcgcgc aactgaaagg cttctatgac gcgattcagg cgctggttgc
     9121 acagcgtaag ctgctcgcat accacgaccg ctctgatggc ggcctgctgg taacgctggc
     9181 ggaaatggcc tttgctggtc attgcggcat taacgcggat attgcctctc tgggcgacga
     9241 tcgcctggcg gcgttgttta acgaagaact gggtgcggtg attcaggttc gtgccgctga
     9301 ccgtgaagcg gtcgagtccg tactggcaca gcatgggctt gctgattgtg tccattatgt
     9361 agggcaggcg gtttccggtg accgttttgt gattaccgcc aacgggcaga ctgtattcag
     9421 cgaaagccgc accacgttgc gtgtctggtg ggcagaaact acctggcaga tgcagcgcct
     9481 gcgtgacaac ccggagtgtg ctgaccagga gcaccaggcg aaatcaaacg acgccgatcc
     9541 gggcctgaat gtgaaactgt cgttcgatat caacgaagat gtggcagcac cgtatattgc
     9601 cactggcgca cgtccgaaag ttgctgtgct gcgtgagcag ggcgtgaact cgcatgttga
     9661 aatggcggca gctttccacc gtgcaggctt tgatgctatc gacgtgcata tgagtgacct
     9721 gctgaccgga cgcacgggcc tggaagattt ccacgccctg gtcgcgtgcg gtggtttctc
     9781 ctacggtgat gtgctgggtg ccggtgaagg ttgggcgaag tcaatcctgt tcaatgaccg
     9841 tgtacgcgat gagtttgcaa ccttcttcca ccgtccgcaa acgctggcgc tgggggtatg
     9901 taacggttgc cagatgatgt ctaacctgcg tgaactgatc ccggggagcg agttgtggcc
     9961 acgttttgtg cgtaacacct ccgatcgctt tgaagcgcgt ttcagcctgg ttgaagtaac
    10021 ccaaagcccg tctctgctgt tgcaggggat ggtgggctcg caaatgccga ttgctgtctc
    10081 tcatggtgaa gggcgcgtgg aagtgcgtga tgcggcgcat ctggcggcac tggaaagcaa
    10141 agggctggtg gcactgcgct atgtcgataa cttcggcaaa gtcactgaaa cctacccggc
    10201 taacccgaac ggttcaccga acggtattac ggcggttacg actgaaagtg gtcgagtcac
    10261 cattatgatg ccgcacccgg aacgtgtttt ccgtactgtc agcaactcct ggcatccgga
    10321 aaactggggc gaggatggcc catggatgcg cattttccgc aatgcgcgta agcagttggg
    10381 gtaagtcatc agttcattgg ttttgcagcc cggatgcggc attaagcacc aaccactatc
    10441 ataagctccc aactccggga gcttttttgt gtctgtaaat cacgacaatg ggtggtttac
    10501 cgtgtcgctt tctggcgaca cttaaatgat tgattttatt gcaatctaat aagtttatct
    10561 taagttgtct ctttttagcg acacagtggc tgatttacat tcaaatagcg aagaagccat
    10621 aaatccctca aagcaacgta taatcagtac gttaccaaac tattttcttt attggcacag
    10681 ttactgcata atagtaagca gtggctcatt caccgactta tgtcagcccc ttcgggacgt
    10741 gctacataaa attcgaatga cgcacaacaa ggtgcctgcc gtccaacttc tgatatcagc
    10801 gtagctatat caaccatcgg gcgaaacgtc gagttaggca ccgccttatt ccataacaaa
    10861 gccgggtaat tcccggcttt gttgtatctg aacttcccct cggttagcat caggctattc
    10921 gcgtctgacg agagtaacac cttgaaacgc tggcccgttt ttccccgctc attacgacaa
    10981 ctggtaatgc tggcattttt gctaattcta ctacccctgt tggtgctggc atggcaagcc
    11041 tggcaaagcc tgaatgcgct tagcgatcag gcggcgctgg ttaaccgcac tacgcttatt
    11101 gatgcccggc gtagtgaagc gatgaccaac gcggcgctgg agatggagcg tagctaccgt
    11161 cagtattgcg tgctggacga ccctacgctt gcgaaggttt atcaaagcca gcgcaagcgt
    11221 tacagcgaaa tgctcgatgc ccacgcaggc gtactgccag acgataaact ttaccaggca
    11281 ttgcgtcagg atttgaacaa tctggcccaa cttcagtgta acaacagtgg tcccgatgct
    11341 gccgccgccg cgcgtcttga agcctttgcc agtgccaata ccgaaatggt gcaggccacg
    11401 cgcacagtgg tgttctctcg tgggcagcaa cttcagcgtg aaatcgccga acgtgggcaa
    11461 tattttggtt ggcaatcgct ggtgctattt ctggtgagtc tggtgatggt gctacttttc
    11521 acgcggatga ttatcgggcc ggtgaaaaat atcgagcgca tgatcaaccg tctgggggaa
    11581 gggcgttctc tgggcaatag cgtttcgttc agtggaccga gcgagttacg ctcggttggg
    11641 caacgtattc tttggttaag tgagcgcctg tcatggctgg aatcccaacg ccatcaattt
    11701 ttaagacatt tatctcatga attaaaaacg ccactggcga gtatgcgcga gggcactgaa
    11761 ttactggctg accaggttgt cgggccgctt acgccagagc aaaaagaggt ggtgagcatt
    11821 cttgatagca gcagccgcaa tttgcaaaaa ctgatcgaac aactgcttga ttacaaccgt
    11881 aaacaggcgg acagtgcggt ggaactggag aatgttgaat tagcaccgct ggtggagaca
    11941 gtggtttctg ctcatagcct gcccgcacgg gctaaaatga tgcataccga cgtcgatctc
    12001 aaagcaacag cttgcctggc ggagccaatg ctgctgatga gcgtactgga taatctttac
    12061 tccaatgcgg tgcactacgg ggctgaatcc ggtaacattt gccttcgcag cagttcacat
    12121 ggtgcgcggg tttatattga tgtcatcaat acaggcacgc ccattccgca agaggaacgc
    12181 gccatgatct tcgaaccctt ttttcaggga agccaccagc gaaaaggggc ggtgaagggc
    12241 agcggtctgg gattaagcat tgccagggat tgtattcgcc gtatgcaagg ggaactgtat
    12301 ctggtcgacg agagcgggca agacgtttgt ttccgcattg aattaccgtc gtcgaaaaac
    12361 acgaaataat gctaacagcc tatcctatca ggctatttta tttgtcagtt cggccccggg
    12421 cagtgctcga aatcttcacg tacgatatgt acgctccggt ttctccgcgc tgtccatgtc
    12481 cagactgtct gcaacaatta cgcccactgg gataggcttt aagtctggtg aatatgcgac
    12541 acatttttca acgattattg ccccgacggc tgtggctggc agggctgcca tgtctggcat
    12601 tgctgggttg cgtgcagaat cacaataagc cagccattga tacgccagca gaagaaaaaa
    12661 ttccggttta tcaactggct gactatcttt ctactgaatg tagcgacatc tgggcgctgc
    12721 aaggtaaatc aaccgaaacc aatccgcttt actggctgcg ggcgatggat tgtgctgatc
    12781 gtttaatgcc tgcgcagtcg cgtcagcaag ctcgccaata tgacgacggc agctggcaaa
    12841 atacctttaa acaagggatt ttgctcgccg acgccaaaat tacgccgtat gagcgccgcc
    12901 agctggtcgc gcgtattgaa gcgttaagta ccgagatccc ggcacaggtt cgcccactgt
    12961 atcaactctg gcgcgacggt caggcgctgc aactgcaact ggcggaagag cgccaacgtt
    13021 acagtaaact ccagcagtcc agcgacagcg agctggatac attgcgccag caacatcacg
    13081 ttctacagca gcaactggaa ctcaccaccc gcaagctgga aaacctgacc gatattgaac
    13141 gccagctctc gacccgcaaa ccgactggaa atttctcgcc ggatacgccg cacgagagtg
    13201 aaaagcccgc gccatccacc catgaggtca ctcctgatga gccataaacc tgcgcattta
    13261 ttattggtcg atgacgatcc gggattgctg aaactgcttg gcctgcgcct gaccagcgaa
    13321 ggctacagtg tggtcacggc ggaaagtggc gctgaaggat tacgggtact gaatcgcgaa
    13381 aaagtagatt tagttatcag cgacctgcgg atggatgaaa tggacggtat gcagctattt
    13441 gctgaaatcc agaaagtgca gccgggaatg ccagtaatta ttcttactgc gcatggttct
    13501 atccccgatg ccgttgctgc aacacagcag ggcgttttta gtttcctcac caagcctgtc
    13561 gataaagatg cgctatatca ggcaattgac gatgcgctgg agcaatccgc gccagccacc
    13621 gatgaacgct ggcgcgaggc aattgtcacc cgcagcccgc tgatgctgcg tttgctggaa
    13681 caggcgcggc tggtggcgca atcagacgtc agcgttttga ttaacggtca gagcggcacc
    13741 gggaaagaga ttttcgccca ggctatccac aacgccagcc cgcgcaacag caaaccattt
    13801 attgctatta actgtggcgc attgcccgag caattgctgg agtcggagct gtttggtcat
    13861 gcgcgtggcg cgtttactgg cgctgtcagc aatcgcgaag gtttattcca ggcggcggaa
    13921 ggcggaacgc tatttctcga tgaaattggc gatatgcctg cgccgttaca ggtcaaactg
    13981 ctgcgcgtgt tgcaggagcg taaagtgcgc ccgctgggca gtaaccgcga tattgatatc
    14041 aatgtgcgga ttatttctgc cactcaccgt gatctgccaa aagcgatggc gcgcggggaa
    14101 ttccgcgaag atctctatta ccgcctcaac gttgtcagcc tgaaaattcc ggcactggcg
    14161 gagcgcacag aagacattcc gctactggca aatcacctgt tgcgccaggc ggcagagcga
    14221 cataaaccgt ttgtccgcgc gttctctacc gatgcgatga aacgcctgat gaccgcgagc
    14281 tggccgggta atgtgcgcca gttggtcaac gtgattgaac agtgcgtggc gctgacctca
    14341 tctccggtga ttagtgatgc gctggtggag caggcgctgg agggtgaaaa tacggcgctg
    14401 ccaacctttg ttgaggcacg taatcagttt gaactcaact atttgcgtaa gctgctgcaa
    14461 atcaccaaag gcaacgtcac ccacgcggcg agaatggcgg ggcgcaaccg gacagaattt
    14521 tataaactgc tttcccgaca cgagctggat gcaaacgatt tcaaggaatg aattggcgtt
    14581 atgtgttacg tttagcagat caaaagacag gcgacctttt caaggaatag catgaaaaag
    14641 attgatgcga ttataaaacc cttcaagctg gacgatgtcc gcgaagcact ggccgaagtc
    14701 ggtattaccg gcatgacggt gaccgaagtg aaaggctttg gtcgccagaa aggccatacc
    14761 gagctgtacc gcggcgcgga gtatatggtg gattttctgc cgaaagtgaa aattgagatt
    14821 gtcgtaccgg acgacattgt cgatacctgt gtcgatacca ttattcgcac ggcgcaaacc
    14881 ggcaaaatcg gtgacggtaa aatcttcgtc tttgacgtgg cacgggtcat tcgcatccgt
    14941 accggtgaag aggacgacgc ggcaatttaa accgcgtcaa gggttgccgg atgtttccat
    15001 ccggcaacat caaattacag caccttatgc gggccaaagc attcgtaatg aatgttttcc
    15061 tgcttcacgc ccagatccac taactgtttc gcggtaaact gcatgaagcc aaccgggccg
    15121 catagataga actgcattgt cggatcgctg aacgcacctt ccagtttgct caaatccatc
    15181 agaccttcgc tatcaaactg acctttagcg cgatcggctt cgctcggctg acgatgccag
    15241 gtgtgcgcgg taaagcgcgg cagtgactgc cccagttcct taacttcatc ggcaaaggcg
    15301 tgaacatcgc cattttctgc cgcatggaac cagttcactt gtgctgtgtg gcctgctttt
    15361 gccagcgtgt cgagcattgc cagcattggc gtttgaccaa caccggcaga gattaacgtc
    15421 actggtgtgt catctgcgac agccataaag aaatcacctg ccggagcgac cagtttcacg
    15481 acatcgccaa cattggcgtg attgtgcaac cagttggata cctgcccacc ctcttcgcgt
    15541 ttcaccgcaa tacgatagcc tttgccatcc ggtttgcgag tcaaagagta ctgacgaatt
    15601 tcctgatgcg ggaaaccttc cggcttcagc cagacgccga gatattgccc cggacggtat
    15661 tctgccactg cgccaccgtc gaccggctcc agttcgaagc tggtgataag cgcgctgcgc
    15721 ggtgttttag ccacaatgcg gaaatcgcga gtaccttccc aaccaccggc tttgctggcg
    15781 ttttcgttat agatttccgc ctcgcgattg ataaatacat tagccagtac accataggct
    15841 ttaccccacg cgtccagcac ttcctgcccc gggctgaaca tttcgtccag cgttgccaac
    15901 aggtgttcac cgacgatgtt gtactgttcc ggtttgatct ggaagctggt gtgcttctgc
    15961 gcgatttttt ctaccgctgg cagcagcgca ggcaggtttt caatattact ggcgtaggcg
    16021 gcaatagcgt taaacagggc ttcacgttga tcgccattac gctggttact catgttaaaa
    16081 atttctttga gttctgggtt atgagtaaac atacggtcgt agaaatgggc ggttaacttt
    16141 ggccccgttt ccaccagtaa agggatggtg gcttttactg tagcgatggt ttgagcgtca
    16201 agcatatggt cttccttttt ttgcatctta attgatgtat ctcaaatgca tcttataaaa
    16261 aatagccctg caatgtaaat ggttctttgg tgtttttcag aaagaatgtg atgaagtgaa
    16321 aaatttgcat cacaaacctg aaaagaaatc cgtttccggt tgcaagctct ttattctcca
    16381 aagccttgcg tagcctgaag gtaatcgttt gcgtaaattc ctttgtcaag acctgttatc
    16441 gcacaatgat tcggttatac tgttcgccgt tgtccaacag gaccgcctat aaaggccaaa
    16501 aattttattg ttagctgagt caggagatgc ggatgttaaa gcgtgaaatg aacattgccg
    16561 attatgatgc cgaactgtgg caggctatgg agcaggaaaa agtacgtcag gaagagcaca
    16621 tcgaactgat cgcctccgaa aactacacca gcccgcgcgt aatgcaggcg cagggttctc
    16681 agctgaccaa caaatatgct gaaggttatc cgggcaaacg ctactacggc ggttgcgagt
    16741 atgttgatat cgttgaacaa ctggcgatcg atcgtgcgaa agaactgttc ggcgctgact
    16801 acgctaacgt ccagccgcac tccggctccc aggctaactt tgcggtctac accgcgctgc
    16861 tggaaccagg tgataccgtt ctgggtatga acctggcgca tggcggtcac ctgactcacg
    16921 gttctccggt taacttctcc ggtaaactgt acaacatcgt tccttacggt atcgatgcta
    16981 ccggtcatat cgactacgcc gatctggaaa aacaagccaa agaacacaag ccgaaaatga
    17041 ttatcggtgg tttctctgca tattccggcg tggttgactg ggcgaaaatg cgtgaaatcg
    17101 ctgacagcat cggtgcttac ctgttcgttg atatggcgca cgttgcgggc ctggttgctg
    17161 ctggcgtcta cccgaacccg gttcctcatg ctcacgttgt tactaccacc actcacaaaa
    17221 ccctggcggg tccgcgcggc ggcctgatcc tggcgaaagg tggtagcgaa gagctgtaca
    17281 aaaaactgaa ctctgccgtt ttccctggtg gtcagggcgg tccgttgatg cacgtaatcg
    17341 ccggtaaagc ggttgctctg aaagaagcga tggagcctga gttcaaaact taccagcagc
    17401 aggtcgcgaa aaacgctaaa gcgatggtag aagtgttcct cgagcgcggc tacaaagtgg
    17461 tttccggcgg cactgataac cacctgttcc tggttgatct ggttgataaa aacctgaccg
    17521 gtaaagaagc agacgccgct ctgggccgtg ctaacatcac cgtcaacaaa aacagcgtac
    17581 cgaacgatcc gaagagcccg tttgtgacct ccggtattcg cgtgggtact ccggcgatta
    17641 cgcgtcgcgg cttcaaagaa gcagaagcga aagaactggc tggctggatg tgtgacgtgc
    17701 tggacagcat caatgatgaa gccgttatcg agcgcatcaa aggtaaagtt ctcgacatct
    17761 gcgcacgttt cccggtttac gcataagcga aacggtgatt tgctgtcaat gtgctcgttg
    17821 ttcatgccgg atgcggcgtg aacgccttat ccggcctaca aaactttgca aattcaatat
    17881 attgcaatat ccgtgtaggc ctgataagcg tagcgcatca ggcaatctta ggtatgtgat
    17941 catcaaggct tccttcggga agcctttcta cgttattgcg ccatcaaatc tgtcgtaact
    18001 gcgcctcaac atacaaatag ccaatcccca acacctgttg tgcgcggctt aattgcccaa
    18061 agccaatttg cgttgctttc acgctcggcc cttcgtcgcg gtcgaacggg ttaaatcccg
    18121 tctggcgaat aatggtatta agccagtgct caccaaaggc acaactgcga ccgtacaacc
    18181 aaatctgatt aatattgagg atgttgagga agttatacag actcaaacca atggcattgg
    18241 cactgcgatc aacccaactg gtgatccacg gttctccact ctaccaggca gcgattaact
    18301 gcgctgtagt cagtttttcc ggatcaagct gagtattaac cggttgtgat tttagccata
    18361 cccgcgcctg tttttttaat gcacttaacg aggcgacggt ttccaggcag ccgtaacgtc
    18421 cacagtcgca gacgacgcca tccggattaa cgatggtgtg actaatctgt ccgctaccat
    18481 acaaactgcc gcgataaatt tgctcgttaa tcacgaacga cgagccaatg ccgtaatcaa
    18541 cgttgatcac gcagaaatcc cgtttctgcg aattattttg ccatttctcc gccagcgcca
    18601 gcatcacgca gtcattatcg accatcaccc gaatgccgag cttctcttcc agcagatact
    18661 tcacctcaac cggcgttgtc cacggcgctt gcggcatggt ttgcgacacg ccggtcaccg
    18721 gatcaacctg accgtggatt gccagcgcca gattgatggt gcggtccggc cacaatttac
    18781 ggtggcgatg ccagcatttt tcgatttcgg acagcagcgc ctgcggcgtc ggtgcatcaa
    18841 tctgtaaata ctcaaattca cctttcggac ttaaacaagc attagcaacc tgacactcaa
    18901 tactggtggg cgtcacgttc aggcacagcg tccagtcacc ttccggcgca atcagccatg
    18961 taccgctact atgcccgcgc gtctggcttt catcgtcgat attcaccacc cgtttttcgc
    19021 tttccagttc ctgcaaaata ttactgactg ccggaatcga aatttgcgcc agccgggcca
    19081 gcgttgattt attggcgcgc ttttgccggt acagcagttc cagaatcacg catttgttat
    19141 ggtggcgaat ctgttgattg ttaatgcagg ctctcatctt cactaaacct tgtttagtta
    19201 attgcgtgat tattccgcga aaacattgcc agattgttca atacactgcc acaaatcttt
    19261 taattcagta tgtcttgtta atattgaggg caccatgact ccagtaaaag tgtggcaaga
    19321 gcgcgttgag atcccgacct atgaaaccgg gccgcaggat atacatccca tgttcctgga
    19381 aaatcgcgtt tatcagggat cgtccggcgc ggtttatccc tacggcgtga ccgatacgct
    19441 gagcgagcag aaaaccctga aatcctggca ggcggtgtgg ctggaaaacg actacatcaa
    19501 agtgatgatc ctgccggaac tgggcggtcg ggtgcatcgc gcgtgggata aagtgaaaca
    19561 gcgcgatttt gtttatcaca atgaagtcat taaacctgcg ctggtggggc tgctggggcc
    19621 gtggatttcc ggtgggattg agtttaactg gccgcaacat catcgcccga ccacctttat
    19681 gcccgttgat ttcaccctcg aagcccatga agacggtgca cagacggtgt gggtaggcga
    19741 aacggagccg atgcatggtt tacaggtgat gacaggtttc accctgcgcc ctgggcggcg
    19801 ctggaaatcg ccagccgcgt ctataacggc aacgccacgc cgcgtcattt cttgtggtgg
    19861 gccaacccgg cagtgaaagg gggtgaaggg catcagagcg ttttcccacc ggatttgact
    19921 gcggtgtttg atcacggcaa acgggccgtc tccgctttcc ccatcgctac cggcacttac
    19981 tacaaagtgg actactccgc tggagtggac atttctcgct ataaaaatgt gcccgttcca
    20041 acctcatata tggccgaaaa atcgcagtac gattttgtcg gcgcgtggtg tcacgatgaa
    20101 gatggcggtt tgctgcacgt tgccaaccac catattgcgc caggtaaaaa acagtggagt
    20161 tggggacaca gtgaatttgg ccaggcgtgg gataagagtc tgaccgacaa taacggcccg
    20221 tatatcgaac tgatgaccgg tatttttgcc gataaccagc ccgattttac ctggcttgat
    20281 gcttacgaag agaagcgttt tgagcagtat ttcctgcctt atcattctct gggcatggtg
    20341 caaaatgcct cccgcgatgc ggtgataaaa ctccagcgta gtgagcaggg gattgagtgg
    20401 gggctgtatg ccatctctcc gttgaacgga taccgcctgg cgatccacga aatcggcaaa
    20461 tgcaacgcgt tacttgatga tgccgtggca ctgatgcctg cgaccgccat ccagggcgtg
    20521 ttgcacggta tcaatcctga taggctgacc attgagcttt ccgatgccga cggcaatatt
    20581 gtactgagtt atcaggaaca tcagccgcaa gcgttgccgt taccggacgt cgccaaagcg
    20641 ccactggcag cacaagacat taccagtaca gatgaagcct ggtttatcgg tcagcatctg
    20701 gagcaatatc atcacgccag ccgttcaccg ttcgattact acctgcgcgg cgtggcgctg
    20761 gacccgctgg attatcgctg taacctggcg ctggcgatgc tggaatataa ccgcgcagat
    20821 ttcccgcaag cggtggcgta tgccagtcag gcgctgaaac gcgcacatgc gctgaacaaa
    20881 aacccgcagt gtggacaagc aagtttgatt cgcgccagtg cttacgaacg tcagggacaa
    20941 tatcaacaag ccgaagagga tttctggcgt gcggtctgga gcggcaacag caaagccggt
    21001 ggctattatg gcctggcacg actggcggcg cgtaatggta acttcgacgc tggtctggat
    21061 ttttgccaac aaagtcttcg cgcctgccca accaatcagg aagtgctttg cctgcataat
    21121 ctgctgctgg tgttaagtgg tcgtcaggac aacgcgcgtt tgcagcgcgc gaaactgctg
    21181 cgtgattatc cgctgaacgc cattctgtgg tggctgaact ggttcgatgg tcgtagcgaa
    21241 tcagccctcg cgcagtggcg cggtctgtgt cagggacgcg acgttaacgc cctgatgacc
    21301 gccgggcaac tgattaactg gggaatgccc actctggcga cagagatgct gaacgcactg
    21361 gactgtcagc gcacgttatc gctttatcta caagccagct tgctgccgaa agccgaacgc
    21421 ggcgaactgg tcgcaaaagc cattgatgtc ttcccgcagt ttgtccgttt cccgaatacg
    21481 ctggaagaag tggcggcgct ggagagtatt gaagagtgct ggtttgcccg tcatttactg
    21541 gcctgtttct actacaacaa gcgcagctac aacaaagcca ttactttatg gcaacgttgc
    21601 gtagagatgt cgccggagtt tgccgatggc tggcgcgggt tggcgatcca tgcgtggaat
    21661 aagcaacacg attatgaact ggccgcgcgt tatcttgata atgcttatca gcttgcgccg
    21721 caggatgcac gtctgctttt cgaacgggat ttgctggata agttaagtgg agccacaccg
    21781 gagaaacgac tggcgcgtct ggaaaataat ctggaaattg cgctgaaacg cgacgacatg
    21841 accgcagaac tgctcaattt gtggcatctc acgggtcagg cagacaaagc ggcggacatt
    21901 ctcgccacgc gcaaattcca cccgtgggaa ggcggggaag ggaaggtcac cagtcagttt
    21961 atcctcaacc agttattacg cgcctggcag catcttgatg ccagacagcc gcagcaggcc
    22021 tgcgaactgc ttcatgccgc gctgcattat ccggagaatt taagcgaagg ccgtttaccg
    22081 gggcaaactg ataacgacat ctggttctgg caggcgatat gcgccaacgc gcagggcgat
    22141 gaaactgaag cgacgcgttg tttacgtctg gcggcgaccg gcgatcgcac cattaacatc
    22201 cacagttatt acaacgatca gccggttgat tatctcttct ggcaaggaat ggcgctgcga
    22261 ctgctgggcg aacaacaaat cgcacagcaa ctgtttagtg aaatgaaaca gtgggcgcaa
    22321 gagatggcga aaaccagtat tgaggcggat ttctttgctg tttcacaacc tgacctgttg
    22381 tcgctgtatg gcgatttaca acagcagcat aaagaaaaat gcctgatggt ggcgatgctg
    22441 gcgtccgcgg gactcgggga ggttgcgcaa tatgaatctg ctcgcgctga attgacggcg
    22501 attaatccgg cctggccgaa agcggcatta ttcaccaccg tgatgccttt tatttttaac
    22561 tacgttcact aaaccctatt aactgtaata tttgagcggc acgcgctgcc gctcatcaca
    22621 aaataattag aataagccgt tgttaataaa aactctacag gaggcactat gcctaaaaaa
    22681 atgagaacaa cccgtaatct attattaatg gctacgctat taggtagcgc actatttgcc
    22741 agggctgcgg ataaagaaat gaccattggt gcaatatacc ttgataccct gggatattac
    22801 gctggagtgc gtcagggcgt tcaggatgcg gcaaaagatt cttcagtaca ggtacagtta
    22861 attgaaacta acgcccaggg tgatatttcg aaagaaagta cctttgttga taccctcgtg
    22921 gcgcgtaatg tcgatgccat tattttatcg gcagtgtctg aaaatggcag tagccgtacc
    22981 gttcgtcgcg ccagtgaagc gggtattccg gtgatttgct acaacacctg tattaatcaa
    23041 aagggtgtcg ataaatatat ctcggcgtat ctggtcggcg atccactgga atttggtaaa
    23101 aaactgggta acgctgccgc cgattatttt attgccaata aaattgacca gccgaaaatt
    23161 gccgtcatca attgcgaagc ctttgaagtt tgtgtgcagc gacgtaaagg atttgaagaa
    23221 gtattaaaat cccgcgttcc cggcgcgcaa attgtcgcta atcaggaagg gactgtttta
    23281 aataaagcga tttccgttgg tgaaaaactg attatctcca cgccggatct caacgccatt
    23341 atgggggagt caggcggtgc gacactcggc gcggtaaaag cggtacgtaa tcaaaatcag
    23401 gccggaaaaa ttgctgtttt cggttcggat atgacaaccg aaattgctca ggagctggaa
    23461 aacaatcagg tgctgaaagc ggtagtggat atttccggta agaaaatggg caatgctgtt
    23521 ttcgcgcaaa cattgaaggt tatcaataaa caagccgacg gtgaaaaagt gattcaggtg
    23581 ccaatcgatc tctataccaa aacggaagat ggtaaacagt ggctggcaac gcacgttgat
    23641 ggtctgccct aatcgctgat tcgcagaggt ccctatgttc acggcaacag aggcagtccc
    23701 ggtagcaaaa gtggtggcag gaaataagcg ttaccccggc gtcgttgcgc tggataacgt
    23761 taacttcacg ctcaataaag gcgaagttcg tgcgctgtta ggtaaaaacg gcgcgggcaa
    23821 atcgactctc attcgaatgc ttaccggcag cgaacgcccg gatagcggtg atatctggat
    23881 tggcgagacg cgactggaag gtgacgaagc tacgttgact cgccgtgccg ctgaactggg
    23941 ggttcgtgcg gtttatcagg aattaagtct ggtggaaggg ctgacggtgg cagaaaacct
    24001 ctgccttggt caatggcctc gccgcaacgg catgattgat tacctgcaaa tggcgcagga
    24061 tgcccaacgt tgcttacagg cgctgggcgt tgacgttagt cctgaacaac ttgtttcaac
    24121 gctaagcccg gcgcaaaagc aactggtgga aattgcacgg gtgatgaagg gcgagccgcg
    24181 cgtggtcatt cttgatgaac ccaccagctc gcttgccagt gcagaagttg aactggtgat
    24241 cagtgcggtg aaaaagatgt cggcactggg cgtggcggtg atttatgtca gccaccggat
    24301 ggaagaaatc cgccgcattg cctcctgtgc caccgttatg cgcgatggtc aggtggcggg
    24361 cgatgtgatg ctcgaaaaca cctccacgca tcatattgtg tcgctaatgc tagggcgtga
    24421 tcacgttgat attgcgccgg ttgcccctca ggaaattgtg gatcaggccg tactggaagt
    24481 ccgtgcgtta cgccataagc ccaagctgga ggatatcagc tttacgttac gtcgcggcga
    24541 agtgcttggc attgctggcc tgctgggggc agggcgcagt gaattgctga aggcgattgt
    24601 tgggctggag acgtatgaac agggcgaaat tgttatcaac ggcgagaaaa tcacgcgccc
    24661 cgattacggc gacatgctga aacgcggcat tggctatacg ccagaaaacc gcaaagaagc
    24721 ggggatcatt ccctggttgg gcgttgacga aaatacagtg ctgaccaatc ggcaaaaaat
    24781 cagcgccaac ggagtgctgc aatggtccac catccgccgc ctgaccgaag aggtgatgca
    24841 gcggatgacg gtcaaggccg ctagtagcga aacgcccatc ggcacgcttt ctggtggcaa
    24901 tcagcaaaaa gtggtgatcg gtcgttgggt ctatgccgcc agccagattt tgttgctcga
    24961 cgagccaacg cgcggcgtcg atatcgaagc caaacagcag atttaccgta ttgtccgtga
    25021 gctggctgcc gaaggaaaaa gcgtggtgtt tatctccagt gaagtggagg agttgccgct
    25081 ggtgtgtgac cgcatcctgt tattacagca cggcacgttc tcgcaggagt ttcactcgcc
    25141 ggtcaatgtg gatgagctga tgtccgccat tctgtctgtg cactgatgaa ttttaccgag
    25201 gaacctgaag atgtctgctt cgtcattacc attgccgcag ggcaagagcg tctcgctcaa
    25261 acaatttgtc agtcgccata ttaatgagat cggtttgctg gtggtgattg ccattcttta
    25321 tctggtcttc tccctgaacg cgcctggctt tatctcattg aataaccaga tgaacgtgct
    25381 gcgcgatgcc gccaccattg ggattgccgc ctgggcgatg acgctgatta ttatctccgg
    25441 tgaaattgat gtcagcgttg ggccgatggt ggcttttgtc tcggtgtgcc tggcattttt
    25501 gctgcaattt gaagttccgt tggcgattgc ttgtctgctg gtgttgctgt taggcgcgct
    25561 gatgggaacg ctcgccgggg tgctgcgcgg cgtgtttaac gtaccaagtt tcgttgccac
    25621 actggggctg tggagcgccc tgcgcggaat ggggctgttt atgaccaacg cactgccagt
    25681 gccgattgac gaaaacgagg tgctggactg gctgggcgga caatttctcg gtgtgccagt
    25741 atccgcgctg atcatgatgg tgttgtttgc gctgtttgtg ttcattagcc gaaaaaccgc
    25801 cttcgggcgc tcggtttttg ccgttggcgg taatgccacg gcggcgcagt tgtgcggtat
    25861 taacgttcgt cgggtgcgca ttcttatctt taccctttcg ggattattag cggcggtgac
    25921 cggcattttg ctggcggcac gcctcggttc cggtaacgca ggtgccgcaa acggtctgga
    25981 gtttgacgtc atcgccgcgg tcgtggtcgg cggtacggca ctttccggcg gtcgcggttc
    26041 cctgttcggt acattgcttg gtgtgctggt gattacgcta atcggtaacg gtctggtgct
    26101 gctcggtatt aactcctttt tccagcaggt ggtacgcggc gtcatcatcg tggtggcggt
    26161 gctggcgaat atcttgctga cccagcgaag cagtaaagcg aaacgctaaa ccttaacgcc
    26221 cctggcgcgg gtgcggcagg gatgaaaatt aatcgtattg ttaatgaggg atttatgaaa
    26281 acgatgctgg cagcttattt accaggaaat tcaaccgtcg atctgcggga agttgcggtg
    26341 ccgacgccgg ggattaacca ggtactgatc aaaatgaaat cctccgggat ttgcggaagc
    26401 gatgtccact atatctatca tcaacaccgt gccacggcgg cggcacccga taaaccgtta
    26461 taccagggat ttatcaacgg tcatgaaccg tgcgggcaga ttgtggcgat ggggcaaggc
    26521 tgccgccatt ttaaagaggg cgaccgcgtg ctggtgtatc acatttctgg ctgtggtttt
    26581 tgcccgaact gccgccgcgg ctttcctatc tcttgtactg gcaaaggaaa agcggcttac
    26641 ggctggcagc gtgacggcgg tcatgccgaa tatctgctgg cggaagaaaa agatctgatc
    26701 ctcctgccgg atgcgctgag ctacgaagat ggtgcgttta tcagttgcgg cgttggcacg
    26761 gcctatgaag ggattttgcg cggcgaagtt tccggcagcg ataacgtgct ggtggtcggt
    26821 ctggggccgg tcggcatgat ggcgatgatg ctggcgaaag gtcgcggtgc aaaacggatc
    26881 atcggcgttg atatgctgcc ggaacgtctg gcgatggcaa aacagttagg ggtgatggat
    26941 cacggctatt tagcaaccac cgaaggtctg ccgcagatta tcgccgaact cacccacggt
    27001 ggcgcggatg ttgcgctcga ttgttctggt aatgccgcag gtcgcttgct ggcactgcaa
    27061 tccaccgctg actggggacg ggtggtttac attggtgaaa ccggaaaagt ggaattcgag
    27121 gtcagcgccg atctgatgca ccatcaacgg cggattatcg gctcctgggt gaccagtctg
    27181 ttccatatgg aaaaatgcgc ccacgatttg actgactgga agctgtggcc gcgtaacgcc
    27241 attacccatc gcttctcgct ggaacaggca ggtgatgcct atgcgctgat ggcgagcggc
    27301 aaatgcggga aagttgtgat taacttcccg gattaaggag ttagcggatg accatctata
    27361 ccttatccca cgggtcgcta aagctggacg tttctgacca gggcggcgta atcgaaggct
    27421 tctggcgcga tacgacgccg ctcttacgtc ctggtaaaaa aagcggtgtg gcaaccgatg
    27481 cctcatgttt tccgctggtg ccatttgcaa accgggtgag cggcaaccgt tttgtctggc
    27541 aggggcgtga gtatcaactg caaccgaatg ttgagtggga tgcgcactat ctacacggcg
    27601 atggctggct gggcgagtgg caatgtgtct cgcacagcga tgatagtttg tgtctggtgt
    27661 atgaacatcg cagcggtgtc tatcactatc gggtaagtca ggcgtttcat ttaacggcgg
    27721 atacgctgac ggtgacgctc tctgtcacca atcaaggggc agagacgctg ccatttggta
    27781 ccggctggca tccttatttt ccgttgtcac cacaaacgcg gattcaggcg caggcgagcg
    27841 gttactggct ggaacgggag cagtggctgg cgggtgagtt ttgcgagcag ctaccgcagg
    27901 aactggattt taaccagctc gcgccgttgc cgcgccagtg ggtgaacaat ggttttgccg
    27961 gatggaatgg tcaggcccgc atcgagcagc cgcaggaagg ttatgccatc atcatggaaa
    28021 cgacgccacc tgcaccgtgt tatttcatct ttgtttccga ccctgcgttt gataaaggat
    28081 atgcgtttga tttcttctgt ctggaaccga tgagccatgc gccggatgat catcatcgcc
    28141 cggaaggggg tgatctcatt gcgctagcac caggggaatc aacaacttca gagatgtcgt
    28201 tgcgggtaga gtggctgtaa tttattaaat gccggataag gcgttcacgc cgcatccggc
    28261 atctttccta acgccgatcg agagaaattg cccccggccc ggtaattgcc aatagcaaga
    28321 acgcgccagc gatactgaca ttcttccaga aattaatcat atttggccca accgcatcgc
    28381 cggtcatatc ccagtaatgg tgaccaatca ccgccgtacc cagcgtgtag aaaataaaca
    28441 gcaccgccag cggacgggtg aaaaagccaa gcacgattaa tatcgcggcg ggcacttcca
    28501 taactaccgc aataatcgct gccagcatcg gcattggcgc gcccaacgag gccatatatt
    28561 ggaccgtacc gtcaaagccc atcattttgg gaaaaccaaa aataatgaaa attaagacca
    28621 cggcgatacg ggcaattaat aacaaaacgg ggcgggcagc tccaaaatca aaataacg
//
LOCUS       DAJTZL010000037        27932 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.037, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000037 DAJTZL010000000
VERSION     DAJTZL010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27932
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.037"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            30..461
                     /gene="tnpA"
                     /locus_tag="OO544_001405"
                     /pseudo
     CDS             30..461
                     /gene="tnpA"
                     /locus_tag="OO544_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(592..1915)
                     /locus_tag="OO544_001406"
                     /pseudo
     CDS             complement(592..1915)
                     /locus_tag="OO544_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
     gene            complement(1915..3531)
                     /locus_tag="OO544_001407"
     CDS             complement(1915..3531)
                     /locus_tag="OO544_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR6767037.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQTGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFILGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            3827..4543
                     /locus_tag="OO544_001408"
     CDS             3827..4543
                     /locus_tag="OO544_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR6767038.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLTPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEYQVEYHLRRLHPEKS"
     gene            complement(4540..6201)
                     /locus_tag="OO544_001409"
     CDS             complement(4540..6201)
                     /locus_tag="OO544_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HCR6767039.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKDTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGLLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(6397..6825)
                     /gene="ibpB"
                     /locus_tag="OO544_001410"
     CDS             complement(6397..6825)
                     /gene="ibpB"
                     /locus_tag="OO544_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpB"
                     /protein_id="HCR6767040.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(6937..7350)
                     /gene="ibpA"
                     /locus_tag="OO544_001411"
     CDS             complement(6937..7350)
                     /gene="ibpA"
                     /locus_tag="OO544_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpA"
                     /protein_id="HCR6767041.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(7344..7416)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            7656..7988
                     /locus_tag="OO544_001412"
     CDS             7656..7988
                     /locus_tag="OO544_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HCR6767042.1"
                     /translation="MIRNVLLAFMICSGMALLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(7990..9204)
                     /locus_tag="OO544_001413"
     CDS             complement(7990..9204)
                     /locus_tag="OO544_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HCR6767043.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYCASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYDFHHRRGVIAE
                     GGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERVSFTYNDHVMHELDPALDLRNV
                     GVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHALAF
                     IGDTLSPKGEKVPELFIVELPQDEAGWKAAGDAPLSGTETTLPAPPRGVVQRRLTFTH
                     HRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQ
                     SAFNWHPSGEWLGFVLDNRIACAHAQSGEVEYLTENHANPPSADAVVFSPDGQWLAWM
                     EGGQLWITETDR"
     gene            9305..10369
                     /gene="cbrA"
                     /locus_tag="OO544_001414"
     CDS             9305..10369
                     /gene="cbrA"
                     /locus_tag="OO544_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance protein"
                     /protein_id="HCR6767044.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQHGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSADILRSVLLKQPEKLNTAYWRATRKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKD"
     gene            complement(10366..11703)
                     /locus_tag="OO544_001415"
     CDS             complement(10366..11703)
                     /locus_tag="OO544_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6767045.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASTLWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(11778..12926)
                     /gene="dgoD"
                     /locus_tag="OO544_001416"
                     /pseudo
     CDS             complement(11778..12926)
                     /gene="dgoD"
                     /locus_tag="OO544_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
     gene            complement(12923..13540)
                     /gene="dgoA"
                     /locus_tag="OO544_001417"
     CDS             complement(12923..13540)
                     /gene="dgoA"
                     /locus_tag="OO544_001417"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HCR6767046.1"
                     /translation="MQWQTKLPLIAILRGIKPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPSDIAVFAVGGVTPE
                     NLAQWIDAGCAGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(13524..14402)
                     /gene="dgoK"
                     /locus_tag="OO544_001418"
     CDS             complement(13524..14402)
                     /gene="dgoK"
                     /locus_tag="OO544_001418"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HCR6767047.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPAHFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFTAGLERGLNTPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVAHQHAITLVAGTSLTARYQQAFQAMGCDVTAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(14399..15088)
                     /gene="dgoR"
                     /locus_tag="OO544_001419"
     CDS             complement(14399..15088)
                     /gene="dgoR"
                     /locus_tag="OO544_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HCR6767048.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            15057..15689
                     /locus_tag="OO544_001420"
                     /pseudo
     CDS             15057..15689
                     /locus_tag="OO544_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312658.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(15735..16547)
                     /gene="yidA"
                     /locus_tag="OO544_001421"
     CDS             complement(15735..16547)
                     /gene="yidA"
                     /locus_tag="OO544_001421"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HCR6767049.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(16662..17060)
                     /locus_tag="OO544_001422"
     CDS             complement(16662..17060)
                     /locus_tag="OO544_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HCR6767050.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQGNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSPQANNDLLSAGMELLKGKLFR"
     gene            complement(17300..19714)
                     /gene="gyrB"
                     /locus_tag="OO544_001423"
     CDS             complement(17300..19714)
                     /gene="gyrB"
                     /locus_tag="OO544_001423"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HCR6767051.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(19743..20816)
                     /gene="recF"
                     /locus_tag="OO544_001424"
     CDS             complement(19743..20816)
                     /gene="recF"
                     /locus_tag="OO544_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HCR6767052.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(20816..21916)
                     /gene="dnaN"
                     /locus_tag="OO544_001425"
     CDS             complement(20816..21916)
                     /gene="dnaN"
                     /locus_tag="OO544_001425"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HCR6767053.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(21921..23324)
                     /gene="dnaA"
                     /locus_tag="OO544_001426"
     CDS             complement(21921..23324)
                     /gene="dnaA"
                     /locus_tag="OO544_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HCR6767054.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            23621..23701
                     /locus_tag="OO544_001427"
     CDS             23621..23701
                     /locus_tag="OO544_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6767055.1"
                     /translation="MTIDKNWLNRSNKDLGRSLRFTHQPV"
     gene            23931..24071
                     /gene="rpmH"
                     /locus_tag="OO544_001428"
     CDS             23931..24071
                     /gene="rpmH"
                     /locus_tag="OO544_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HCR6767056.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            24088..24447
                     /gene="rnpA"
                     /locus_tag="OO544_001429"
     CDS             24088..24447
                     /gene="rnpA"
                     /locus_tag="OO544_001429"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HCR6767057.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            24411..24668
                     /gene="yidD"
                     /locus_tag="OO544_001430"
     CDS             24411..24668
                     /gene="yidD"
                     /locus_tag="OO544_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HCR6767058.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            24671..26317
                     /gene="yidC"
                     /locus_tag="OO544_001431"
     CDS             24671..26317
                     /gene="yidC"
                     /locus_tag="OO544_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HCR6767059.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISTFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            26423..27787
                     /gene="mnmE"
                     /locus_tag="OO544_001432"
     CDS             26423..27787
                     /gene="mnmE"
                     /locus_tag="OO544_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HCR6767060.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGLKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVEVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
BASE COUNT         6722 a         7550 c         7123 g         6537 t
ORIGIN      
        1 taaatgcttg tagtcgtttc acaggccaaa tgatactttg gtctgtgaaa aaagaaaccg
       61 atatccggcg tggaaggcat tgtgttttcc tgatgcatgt tcacctggtc tttgtcacca
      121 aataccgacg tcagattttt gactatgacg cgacagaaaa actacgcact tacttttcaa
      181 atgtatgtgc tgattttgaa gctgaactgg tttaaatgga tggcgaacca gatcacgtcc
      241 atttgttaat taactatcct cccaaactgg cgatatccag tctggtaaac agcctcaaag
      301 gcgtatcggg taggttactg cgacgagatc gaccagatat tgcagtcagg tattactaca
      361 aaggcgtttt gtggagtcct ggctattttg ccaatagctg cggaggtgcg ccaatatccg
      421 tcatccgcca atacattgaa caacagcaaa cacctggtta ggtggaaaac cgcgccttat
      481 atccccgccc tgaaggacgg ggttttacgg cgcaccggat aaatttggtt atgcgccgtc
      541 agccgtgcta aagaatacgg accagcaccc aacggatgcc agtccacaca attaacgtaa
      601 ctctggccag tacgctttgt tggcctccag caactcatcc agaatagctt tggcgaccga
      661 ggcgctcggt accgttttcg ataacgtaat cgcctgccac aggtgctgat atgaacgctg
      721 ttcccaggcg tccacgacca gtttttccac cgccacttgc tgactcatta accctttctg
      781 aaactgcggg atatcgccga ccaccagtgg ttctggtccg ttgtgcccga ccagacacgg
      841 gatctcgacc atcgcttcat catcaaagtt atggattgcc ccgttgttag gcacaatcag
      901 caacatccgc tcctgagtgt tgaaggcaat tgccgccgcc agatcgacga tgtatgacgc
      961 atgttcgtca atctccagct tggccgccgc tgaatttcct gccgctgtaa tggcgcggca
     1021 agcatcgaaa acctgtttct cgcgatgttc cattcacctc attcgcgcgg gtatgttcag
     1081 ggttggaatg ctgaacaaca taatccggga agagataata tttcagatag gtgttcggca
     1141 atgtatccgg gtccagtgcc tggacatccc gcgcttttgc ataggtgtcg ttccagctcg
     1201 cttcagtatg tgtatcttgc tgcggtggaa cataaccgtg cttcgagaca tattgccgca
     1261 gctggggcat caggtcgttg ccctgcaaat cttcaattga tgtccaccag ccaaagtggt
     1321 tcaggccgta gtagcgcacg cgcatctgtt tgcgatcttg cagcccaaca atttgcgcca
     1381 tccggctttc aataccgatt ggcatgtcac agatgttgag gattttcgca ttcgggcgca
     1441 gacgacgcgt ggcttctgcg acaatggctg ccgggttgga gtagttgagc atccaggcat
     1501 ttggtgaata tttttccata taatccacca gttccaggac gccgccaatg gaacgcatgc
     1561 cgtacgctat tccgccgggt ccgcaagttt cctgaccaac aacaccgtgg cgcagtggga
     1621 ttttttcatc cagttcgcgc atcgggtact tgccgacgcg gatgtgcgcc atgacaaaat
     1681 caacgtcgct gaacgccact tcaggatcgg tggtgtaact aaacgcaatg tccggcgctt
     1741 tctctttaag gatgacttta caggcttcgg caatcacttc ctgccgcgcg ccatcgttat
     1801 cataaaattt cagtgcacga agcgggaaac gctcctgatt cgccaggagc atcaacacga
     1861 tgcctggcgt aaaggtgctt ccaccgcctg cgacaaccac tgagaatttg gtcattatac
     1921 tgcctccgta atggcaacat tttctgctga ttgatgagaa ttaattaagc tatcgagctg
     1981 ttcacgcagc tgggatacat gcagaccaat gatcacctga atggcatcgc cactacggaa
     2041 gacgccgtgc gctcccagct ttttaaagac ttcgtcatcc agcgtttgtg acatgtcatg
     2101 cagtgcaatg cgtagacgcg tcgcacaatt gttaatgctg gagatattgc cgaccccgcc
     2161 cagggcttgc aggataccgg cagcctgatc cagctctttt tttggctctg ccgcggtggt
     2221 ttggcctcgc gaggctttgt attcggcttt tgagtagagt ttcacttccg catcttcacg
     2281 tcccggcgtg cacatgttga actgcagaat cagggtgcgg aaaaccacga agtacagcag
     2341 ggtaaagcac aacccaatgg cgatttgggt cagcatcata tccgcgtggt tgctgaacat
     2401 cgggatccag ttttgcggta aaacctggtc aatcagacct ccgcccatgt tgcccaccac
     2461 accaaagaga tacatcacgg tcgacattga ggccgcaagc acagcgtgta ccgcaaacag
     2521 caacggtgaa atgaacagga aggtaaattc cagcggttcg gtaattccca ccagcatggc
     2581 ggttaaggtt gcgggaatca gcaagcccgc cacttttacc cgattttccg gtgcggcagt
     2641 gaagtacatc gctaaagaaa tgcccacggc accaaagatt tttgagttac cgtgcagggc
     2701 aaaaccgcct tccgggaaca acgatttcag cggctcggcg ctcaaactga actcttgcag
     2761 atgctgcgcc cagtacatct gaatgccgcc ttcaacagct gccggaccca agataaacgg
     2821 tccgtagatg aagtggtgta aaccggttgg gatcagaata cgttcgagga aggtgtaaac
     2881 ccagacccca agcgcacccg ccgaacgcag gaacgcttgc agagattcaa tccccgtttg
     2941 tacttttggc cagccgagca atgtcagcca ggcacagggg atcatcacca ggaaggcgat
     3001 aatcaccaca taagacgtcc cctggaaaat gccgagaaaa accggcagtt ttttatcgaa
     3061 cagacggtta tgcagcgccg tcacaatgcc ggaaatgata attgcgccga taatgctggt
     3121 atcgagggtt ttaatcccgg ccatcattgt cagaccgcta cctgccaccg cgtcctgagt
     3181 gaaatcgacg ccgaagtagc ttccccaggt cattcccatc gcgttgatga aatagttcca
     3241 ggtcaggaaa ctcaccatca ccgccagaca agcacgtccc tgcgcttgct tagcaaggcc
     3301 aatgggtaaa ccgacagcaa aaatcagcgg catattacgg aataccgtcc aaccgccctc
     3361 ttcaataatg tgtacgattt gcgcgaataa actgttcgga tcggtcagtg attccccgac
     3421 aaacatcggg ttttgcagca agatggcaag acccaccaca atcccggcga agggaaacag
     3481 cagcactggc gtaaacatcg cgccgccaaa gcgttgaatt tgactgagca tttccacatc
     3541 ctcttttctc aattctgtaa gtaggaagcc tgattggtta tgcggcaggc ctggagcaag
     3601 gataggaaga tgcgatcggc agaacatgac gacccaatgg gattcgtgat cgctttcatg
     3661 cttttcgttg ggcggtagag atctacgttt tcgcgaaaaa gtagacatct gtgagcacag
     3721 gtacacagca tcgaacacat ctttaaaaaa aagatgtttt ttcaatcgat taagcagaac
     3781 ttgtgtgcgc attacccggg cttgcaggca aaaaagagat ctagagatga tctacaaaag
     3841 cattgcggag cggttaagaa ttcgacttaa ctccgcagat ttcacgctaa acagccttct
     3901 tccgggtgaa aaaaagctgg cggaagagtt tgcggtatcg cggatgacca tccgtaaagc
     3961 cattgacctg ctggtagcgt gggggctggt ggtccgccgc cacggtagtg gcacttacct
     4021 ggtgcgcaaa gatgtactgc atcaaaccgc cagtctgacc gggctggtgg aggtgttaaa
     4081 acggcaggga aaaactgtca ccagccaggt gctgattttt gaaatcatgc ctgcgcctcc
     4141 ggccattgcc agccagttac ggattcaaat caacgagcag atctacttct cccgtcgcgt
     4201 tcgttttgtg gaagggaaac cgctgatgct ggaagacagc tatatgccgg taaaactgtt
     4261 ccgtaatctt tcgctgcaac atctggaagg gtcgaagttt gaatatattg aacaagagtg
     4321 cgggattttg attggcggta attatgaaag cctgacgccg gtgctcgccg atagactgct
     4381 ggcgcggcaa atgaaggtag cggaacacac gccactgctg cggatcacct cgttgtcata
     4441 tagcgagagc ggggagtttt tgaattattc agtgatgttc agaaatgcca gcgaatacca
     4501 ggtggagtac catttacggc gactccaccc ggaaaagagt taaccgatac tccagaagag
     4561 caccgccagt aattgggggg tgataatgcg caggaacatc actaacggat agacagtggc
     4621 gtaagagagc gccgccgcac cgctagttgg atgaagatta ttagcaaacg ccagcgccgg
     4681 aggatcggtc atggaacctg ccagcatccc gcacatggtc aggtaattca ttttggctaa
     4741 catccgcgcc agaatgccaa cagtaatcag cggaacggcg gtgatcaggg caccataacc
     4801 aatccagctt agcccttcgc cattgaccag ggtattcaca aaatccccac cagatttcag
     4861 accaacgacc gagaggaaca gcacgatccc cagctcccgc agcgcgaggt tggcacttgg
     4921 cggcataaac cagtacagct tgccgatact gccgatacgc ccgaggatca acgccataat
     4981 cagcagaccg cccgccagcc ccagtttcaa cgcggccggg aatcccggca caaagacggg
     5041 aatagaacct aacaatacgc caagcccgat gccaataaac actggcagca tctgaacctg
     5101 ttgcagtttt tgctgcgcat tccccagcac attggcaacg gcatcaattg cggacggacg
     5161 ccccaccagg ttgaggatat cgccgaactg caggctgata tcgccactgg cgaccagttc
     5221 gaccccggca cggttcaggc gcgagataac aacgtcatag cgttctttaa agtgcaggtc
     5281 gcgaatacgt tttccgagca cgttttcatt ggtgaccacc acacgctcga cgcgcaaatc
     5341 agtgtctttc gtggacagcg aagtatcgac ctcctgacca atcaccagtt gcgcattatg
     5401 taaatccgct ggctgaccca ccagatgcag caaatcgccc agttggataa tggtatctgg
     5461 cgaaggaact tttagggttt cttcgcgttt cagacgcgag cagataattt tgtcgccgtt
     5521 gagaatcggt acatctttaa tggctaaatc atgcaggtta gggttctcaa cgcgaatatt
     5581 gatagtcttg atcagcgcgc cgccattggt gcgtgaagac tcgtgctgct gagcttctgt
     5641 ctcgacattg acgcggaaaa taacccgcaa catccacatg gtgaacaaaa tcccgcaaat
     5701 gccgaatgga tacgccatcg cgtaactcat ccccatctga tcgaccattt ccattggtgt
     5761 acccaggtcg cgcaaaatct gctgccctgc ccccagcgct ggcgtattgg taaccgcacc
     5821 ggagaaaatc cccagcacta ccggcagtgg aatatcaaac agtttatgca ggatggcggt
     5881 aaccagacca ccgatgatga cgatcagaac agcaaacagg ttgaggcgta atccggagac
     5941 gcgcaatgag gcaaagaagc ccggccctac ctgaatcccg atggtataaa cgaacaggat
     6001 caggccaaat tcctgaataa catgcagcat atcgctactt aatgtcatcc ccgcctgaga
     6061 aacaaaatgg ccgacgatga tcccaccaaa cagcacgccg ccaataccta atcctatgcc
     6121 gcgaaatttg acgttgccga taaacaaacc gacgactgcc accaaagcca gaatactgac
     6181 cgttaatgct atatcactca tcgtccattt ctcttgagat aacattttaa ttgctacgga
     6241 ttatggctga atcggtgaag cagatttgcc ttatagcgca cattattacg ggaaagaacg
     6301 tgccgaaata tcttaaacag tcgaaattta acgcattgtt gaagaagaaa gacaaaaaaa
     6361 gccccaccag aatggcgggg caaagagaat agctagttag ctatttaacg cgggacgttc
     6421 gctgatagcg atacgctgcg ctgcgatggg ttcaggctca ttacgaatta aatcaatatg
     6481 cagtaaaccg tttacgaaag ttgcgccaga gacttccata ttttcagcca gcgtaaagct
     6541 caggctaaat ggctgattca taagcccttg atgcagccat tttttctctt cttttggctg
     6601 ctccggcgtg ccttttacgc tcaggcgcgt accttccagt tgaatctcta aatcttcctg
     6661 acggaaacct gccagcgcaa gggtaatgcg gtagtggtta tcgtcgcttt tctcaatgtt
     6721 gtacggcggg aagctctgac tttcaccggc gttttgcagt gcgttggcca gtttgtcaaa
     6781 accgatccat tgacgcatca gtggggataa atcgaagtta cgcatagtca tttctccttc
     6841 taagaagcga gtaagtacct gcaaatccga agattcgcat atgctccctg acggcgagca
     6901 tggagatgtc aggccgcgcc aggcggcctt aaggaattag ttgatttcga tacggcgcgg
     6961 ttttttcgct tccggaatca cgcgttcgag atcgatatac agcaaaccat ttaccaggtt
     7021 agcgccacga acatgaatgt tctcagctaa ctggaatttg cgttcaaagt tgcgttcagc
     7081 gatgccctgg tacagatagg tgcgctcttt ttgttcgtcg gcgtgagcac ctttcaccac
     7141 cagcagatta tcctgggcgg taatttccag ttcgctctca gcaaaaccag ccacagcgat
     7201 agcaatgcgg taatggtttt cgtctaccag ttcaacgtta tacggagggt agccgccatt
     7261 actctggctc tggttgtttt ctaagtggtt aaacaaacgg tcaaatccaa tagcagaacg
     7321 gtaaagcggg gataaatcaa agttacgcat aatcaatagc tcctgaaatc agcgagaatg
     7381 taagaccttc cacaatggac aggtcaggta gccagaacac ccatcaggcg cgtcctcatc
     7441 gactacgatg taaaaatggg tctggaaatg aacttttcaa gccttatcag cggacttttt
     7501 tttgcagttt atggtctatt gcatagactg agggggcagc acgaaacgtt aaagtgtgat
     7561 agccgccaca gagcgacgta atgatgatgt tattttcagc aagggatcgc tataactcat
     7621 caggccaaca cccacgatca acagatgaat ttattatgat aagaaatgtg ttgttagcgt
     7681 tcatgatatg cagcggaatg gcattactcg gaggatgctc cagcgtgatg tcccataccg
     7741 gagggaaaga aggaacgtat cccggcaccc gcgccagcgc caccatgatt ggcgatgatg
     7801 aaaccaactg gggcactaag tcgctggcga ttctcgatat gccgtttacc gctgtgatgg
     7861 acacgctttt attaccgtgg gatgtgttcc gcaaagacag ctcggtgcgc tcacgcgttg
     7921 aaaaaagcga agccaacgcg caagccacca atgcggttat tccaccggcc agaatgcctg
     7981 ataactgatt tagcgatcag tttcggtgat ccacagctgg ccaccttcca tccacgccag
     8041 ccattgacca tccggcgaga agaccacggc gtccgcagaa ggtggattgg cgtggttttc
     8101 ggttaaatac tcaacctcgc cgctttgcgc atgggcacaa gcaattcgat tatccagcac
     8161 aaagcccaac cattctcctg acggatgcca gttaaatgca gactgaatat ccgttttgtt
     8221 atgggttaac tggcgcggct cgccgccctg tggcgagata agccacagtt gcacaatgcc
     8281 gttatcatca cgcattaaaa acgcgatttg cgtaccctgc ggattacagc gcacccagtg
     8341 gcgcgggacg ttgactaacc ccggataagc ccgatggtgg gtaaaggtta aacgtcgctg
     8401 cacgacgcca cgcggtggcg cgggcagggt ggtttccgtt ccacttaacg gcgcatcacc
     8461 tgccgctttc cagccagctt catcttgcgg caactcaacg ataaacagct ccggcacttt
     8521 ctcacccttt ggcgaaagtg tgtcgccaat aaacgccagc gcgtgatttc ctacccatcc
     8581 ttcttcataa gcacgattga tttcatcact gccaggctgc ggcgtgggcg tggttttact
     8641 caccagcaca caccagtggc tgccgctgta ttcacgcgga tgctgctttt gtacgttgac
     8701 cggaccaaac ggcgcagcaa caccgacgtt tcgcaaatcc agcgccggat cgagttcatg
     8761 cattacatgg tcgttatagg taaagctcac cctttcaccg ttcgggctaa agacatgcac
     8821 atggctgccg ccgcgcagcg ctcctggcgt atacggcgcg gtaatatcca ttgcatcgag
     8881 attgctcacc ttgccgcctt cagcaatgac tccgcgccga tgatggaagt cgtaatgcca
     8941 tgtttcatca ggattttccg ggccgtgaat aaagacatat ttctctgact tcggatgaac
     9001 ggtcaccacg ccgacgtgtg cgccctgtga cgcacagtag ataacctcga cctcaccggt
     9061 atggatattc acacgctcaa tggtctcgcc ggtaaacgac gccccagaag gacgcacgtc
     9121 aaataccagc cactggctgt cgggcgtcca ggtattggta ttggtgagca ggtgattacg
     9181 gggagcaaag gttatctgtt tcatggcgcg gtcctgatac aaaacgggtc cagccatcat
     9241 acttcacaag gacttcactt tcagacaata gtcttttgcc atcgtgatga atgtgagtga
     9301 aaagatggaa catttcgacg tggcgattat tggcctcggc ccggcagggt cggcgttggc
     9361 acgaaagtta gccggcaaaa tgcaggtgat cgcgctggat aaaaagcacc agcatggtac
     9421 tgaaggtttc agcaaacctt gtggcggtct gctggcaccg gacgcgcagc gatcttttat
     9481 tcgcgatggg ctgacgcttc ctgtcgatgt gatcgctaat ccgcagattt tcagcgtcaa
     9541 aaccgtcgac gtcgccgcat cgctcacgcg taactaccag cgaagctata tcaatattaa
     9601 tcgccatgct ttcgacttgt ggatgaaatc gctgatcccc gccagcgttg aggtttatca
     9661 cgacagcctg tgtcgaaaaa tctggcgtga ggatgataaa tggcatgtca tttttcgtgc
     9721 agacggctgg gagcagcata ttaccgcccg ctatctggtc ggtgccgatg gtgccaactc
     9781 gatggtgcgg cgacatctct acccggatca tcaaatccgt aaatatgtcg caatccagca
     9841 gtggttcgca gagaaacatc cggtgccgtt ctactcctgc atctttgata atgcgataac
     9901 tgactgttat tcatggagta tcagcaaaga cggttatttt atctttggcg gtgcctatcc
     9961 aatgaaagac ggtcagacgc gtttcacgac tctgaaagag aaaatgagcg cctttcagtt
    10021 ccagtttggc aaagcggtga aaagcgaaaa atgcacggtg ctgtttccct cacgctggca
    10081 ggattttgtc tgcggtaagg acaacgcctt tctgattggt gaagcggcgg gatttatcag
    10141 cgccagctcg ctggagggga ttagctatgc gctggatagc gcagacattc tgcgttcggt
    10201 gttactgaag cagccagaga agctcaatac cgcttactgg cgcgccaccc gcaaactgcg
    10261 tttaaaactc ttcggcaaga tagtaaaaag ccgatgcctg accgcaccgg ctttaagaaa
    10321 gtggattatg cgcagtggtg tggcacatat tccacagttg aaggattagc caacgcgctt
    10381 cacatcgccc accagcagga tataagagag cgcgccaatc aacgcgacgg cggagatata
    10441 aaccagtgca ggtgcgaaac cgtaaccctg cgccaggtac cccaccacca gcggaacggt
    10501 gatgccgccc agaccaccgg cgaagttaaa cacgccgccg gttaaaccaa tcaggcgcat
    10561 cggtgccaga gaagaaacca gcgaccaggt aattgaagca aaaccgttac cgaagaatgc
    10621 cagcgccatc aggcacataa tcatcatcgg atcgttagtg tagttagcgc ccataatgca
    10681 ggtggagatc agcaagccgc agataatcgg cgttttacgc gcaaagccca gtgaaaagcc
    10741 cttacgtacc agcagatccg ctacccagcc agagagcagg acgccgacaa acgccgcgag
    10801 gaatggcacc gtggtcataa agcctgcttt cagcgccgtg attccttttt cctgggttaa
    10861 atagttcggg aaccaggtta agaaaaacca cagtgtagaa gccaccgcaa attgcccaag
    10921 atagacgccg atcagtttac gatggaacac cagtttccag tctttggctg ttaacggctg
    10981 acgtgcctct ttcttcactg gcgcatcacc atccaccaga ccgccgccat cacgaatgta
    11041 atccagttca gctttgctga tgcctttggt caggcgcggc ggctgataaa ccttaaacca
    11101 aatcagcgac cagataatgc cgataccacc agtcacaatg aacacccagt gccagctcaa
    11161 catctcctga atccagatca gcagcggagt caaaaacgcc agaccgacaa actgaccaga
    11221 cgtataaaaa ccaacggcgg aggcgcgttc atgttccggg aaccagctgg tcaccatccg
    11281 gttattggtc gggaaagcgg gcgcttcgaa aataccggtt atcgcacgca gaccaattaa
    11341 tgacattaat cccgtggcaa agccctggaa taaagtcgcc actgaccagc caaatatcgc
    11401 aataaaataa gtcacgcgag aacctacgcg atctaaaaac caaccgccgg ggatctggca
    11461 tagcgtataa agccaggcga aggccgaaaa tacatagccc atttccgctt tcgtaatgcc
    11521 gaactcttcc tgaatatggg cggaagccac ggccaggtta gcgcggtcaa cataacaaat
    11581 gaccaccgta ataaagatca tcaccagcgt cagataacgc cgacgccccg gctttgctgc
    11641 attaacggga atatccatag cgagctttct ccagattttg ggcatagcga agccgctcac
    11701 catgccctgt aatttacaga gggttatatt tttgtattgc tgttttagtg cccgatgagg
    11761 ggcttacgtg gcaggaatta ccactctgct acgctgttat cttcatgacg ccagagtgga
    11821 ttacgccagt ccggggcatt tttactgaac tcaataactt tagcttcgtc gatttccacg
    11881 cctaagcccg gtttcgttag cggtttaaag aagccgccaa ccatgctgaa gtcttctttg
    11941 tttttcacaa agtcgagtaa ctccgcgcct ttgttgtaat gaattcccat actttgttcc
    12001 tgaagtaccg cgttatagga aacaaagtcg atatgcagac aagccgccag tgcaatcggt
    12061 ccgagcggac agtgcggcgc aagggtcacg tcataggctt ctgccattcc ggcaattttg
    12121 tagcattcgg taataccgcc tgcatgggag agatccggtt gtagaatcga aataccgcct
    12181 gcctccagca cgcgtttaaa atcgaaacgt gagaacatgc gctcacccgc cgccagtgga
    12241 atatgcgttt gtgccgccaa tttcgggtag tattcggctt gttccgccag caccggctct
    12301 tcaataaaca gcgggcgata cggctccagt tctttaatca gcactttcgc cattggcgcg
    12361 ctgacgcgac cgtggaaatc aagaccaaac tcaatctgat tgccaaaagc ttcacgaatt
    12421 tgtgccacgg tgttgactgc cgcatctacc gcgcgggagt tatcaattag ccccagttct
    12481 tcacaaccgt tcagtttgaa ggtatcgaag ccgatttcgc gcagggtttt aataccgtcg
    12541 atcacatccg ccggacgatc gccgccgacc caactgtagg ctttaatttt gtcgcgaacc
    12601 aggccgccca tcagttgcca gaccgacgca ttcagcactt tacctttgat atcccataac
    12661 gcctggtcga tcccggcaat ggcgctcatc aggattggac cgccacgata aaatccggcg
    12721 cgatacatca cttgccataa gtcattgatg cgctatggat cctgaccaat caaatagtca
    12781 cccagctcgt gaactgccgc ttccaccgtg cgggcgcggc cttcaatcac tggctcgccc
    12841 cagccgacga cgccttcatc ggtttcaatt ttcaggaaca tccagcgggg aggtaaacga
    12901 tacgtggtaa ttttggtgat tttcattgca ctgcctctcg atacgcctta acaaatgctg
    12961 ctgcctgctg cgcggtgcgt tctacggact gcccggcgcg atagagatcg ctgcctaagc
    13021 ccgccccagc acaacctgcg tctatccact gcgccaggtt ttctggcgtc acgccgccaa
    13081 cggcaaagac tgcgatgtcc gatggcaata ccgcttttaa cgctttgatg tattgcggac
    13141 caaaagccga tgacggaaat attttcagcg cctgcgcgcc cgcttcgagc gcggtaaagg
    13201 cttcggtcgc cgtcgcgcag ccggggcaga cggtcatgcc gtagcccacc gcacggcgga
    13261 tcacttcact atggatattg ggcgtaacga tgagctgaca gcccatcctg gcgagcgcat
    13321 cgacctgttc aggtttcagt accgtacctg cgccaatcaa cgccttgtcg ccatacgcat
    13381 caacgatggc gggaatgctt tgctcccatt gtggggaatt cagcgggatt tcaaccgcgt
    13441 cgaacccggc gtcaatcacc gcgccaacat gcgccagcgc ctcgtcgggc ttaatgccgc
    13501 gcaaaatggc gatcagcgga agtttagttt gccactgcat gagcgatgct ccttatacca
    13561 gcctgaaatg ccgtgtcgcc cgccaccgcc gtcacgtcgc aacccatcgc ctgaaaggct
    13621 tgctggtagc gcgcggtcaa cgatgttccg gcgacaaggg tgatggcgtg ttgatgggcc
    13681 acatagtcgc gcatactggc gacctctgcg ccaatcaaca aaccagagag aaattcactg
    13741 acctgttcac ggggaagtgt tcccagcaca tgcgaggcac gaacttcaaa aagctgcggc
    13801 aatatggcgg gcgtattaag accacgctca aggccagccg tgaaggcatc ggcagagttt
    13861 tcctgcggcg gcaaacctgc accaatcaat gagtgattta acagtaaatg atgtaattca
    13921 ccggtcatca cggtgcgaaa atcgttgatt tgctggctat cggcctgcac ccatttgcaa
    13981 tgcgtaccgg gcatgacata aagagaggaa ggagccagag ctcgcgcgcc gatcaattga
    14041 gtttcttcgc cgcgcatcac attgtggtta tcatcatgag agacgcataa tccgggaata
    14101 atccagatat tgtcgccaac agacgttaat tgttcgccaa tagacgaaaa atgggcagga
    14161 acagataaat acggagcaac tttccagccg acattgctgc cgaccattcc tgccattacc
    14221 actggcgtat tctcttcacg ccagtcggtc gtgacttctg ctaacaccgc agccggagat
    14281 tttccgttca ggcgcgtgac gcctgcttct gattgcctgc tctccaggca gtggtcgccc
    14341 tgataaagcc aggcgcgcag attggtcgat ccccagtcaa ttgcgatgta gcgagctgtc
    14401 atgtgatttc ctttaacctt cgtgtcgagc tggcgatcat ggtaagcgcc gcctgctctg
    14461 ccgcatcgcc gtcctgatgc cgtatcgcat cgaacagcgc cttatgttcc tggagcgttt
    14521 gcggcatgtt ggcctcatcg cccatccagg ttcgttcaaa aaccgcccgc tgcagcgaac
    14581 tgatcgcaat gctaagttgc tgtaacaccg ggttatgcac cgactgcagc accgcctcgt
    14641 ggtagcgaat atccgcttcg ttaaacgctt cgcggtcctg attgttggca atcatctcgt
    14701 tcagcgccga ttcaatctgc gccagatcgc tggaggtcgc gcgctctgct gcccaacggg
    14761 caatcgccgg ttccaccaga tttcgcactt cgctcatggc actgataagc cgtgggtcgt
    14821 agtcgttttc cagcacccat tgcagtacgt cagtgtcgag gtaattccac tggttacgcg
    14881 gtgccacaaa cgcgccgcga taacgtttca tttcaatcag ccgtttcgcc atcaacgaac
    14941 ggaacacctc acggatgatg ttgcgcgagg ttgcaaactc ctcacagagt tccgcctcag
    15001 ccggaagcgg cgagcctggt acgtatttgc cgtgaacgat ctgtttaccc agcgtaatga
    15061 cgatgcgatc ggttttattg agagtcatgg agagtccttg tgctctgtat gttcttctct
    15121 actttacccc gatcgatgca taatcaggaa agtatcaatc gcagtcagac accatcacgg
    15181 ggctatcgca ggcaaaggat agtaatggaa caaaaggcta tgtttttgta ggggaatcgc
    15241 tggattacct tatcacagat ggtgccgatg acatcgttaa gatgctcaag gatccagctc
    15301 ttaaccggca caatattcag gttgctgatg acgcaagatt tgttttaaat gcggggaaaa
    15361 agaaatttac cggcacaata tcactctatt acaactggag cacagaagaa gaaaaagcac
    15421 gagtgacaca atatcgtttt gcttgtggtg ttcagcactg tacccggaca gtagaaaacc
    15481 tggaaggtac tatccatgag aaaaataaaa acatggatta ctcaaacgta atggctttct
    15541 accatccatt taaagtgcga ttttatgaat accattcacc cagaggcatt ccggatggtg
    15601 tttccgcagc attactgcca gtgactgtta cgctggacat cattactgca ccgctgcaat
    15661 ttctggttgt atatgcagta aaccaataat cagtaagcgg gcaaacgcgt ttatgctgtt
    15721 tgcccgccca cagattaatt cagcacatac ttctcaatag caaacgccac gccatcttca
    15781 aggttagatt tggtgacaaa gttcgccact tctttcaccg aaggaatagc gttatccatc
    15841 gccacaccga cgcctgcata ttcaatcatt gcgatgtcgt tttcctgatc gccaatcgcc
    15901 atgatttctt ccggtttaat acctaacacg tcggccagtg atttcacacc cgtacctttg
    15961 ttaacgcgtt tatcgaggat ttcgaggaag tacggcgcac ttttcagcac ggtatatttc
    16021 tctttcactt cctgcggaat acgcgcgata gcctggtcga ggatggcggg ttcatcaatc
    16081 atcatcactt tcaggaactg ggtattgggg tccattttct ccgcttcgca gaacaccagc
    16141 ggaatagtgg caacgaaaga ttcatgcact gtgtagtagc tgatatcgcg gttggcggtg
    16201 tacagcgtgg tgcggtccag ggcgtggaaa tgagaaccga cttcgcgaga gagtttttcc
    16261 aggaaacgat agtcgtcata gctgagagca gtttgcgcca cggtgctacc atcagctgcc
    16321 ttctgtacta gcgcgccgtt ataagtaatg cagtagtcgc ccggctgttc catatgcagc
    16381 tctttcaggt agttgtgcac acctgcatac gggcgacccg tcgttagcac gacattcacg
    16441 ccacgggcgc gagctgcggc aatcgcattt ttaacggcgg gtgaaatggt gtgatcgggc
    16501 agcagaaggg tgccatccat atcgatagca atgagtttga tagccatgag ttccccaggt
    16561 agattggttc ctgacccatg ctaacgcgat tccgctcaaa aatcagtaca acacccgagg
    16621 gaaaaggggg atgcaacgcg ggttgcatgc tccccatttg cttagcggaa gagtttccct
    16681 ttcagcagtt ccatacctgc ggaaagcaga tcgttattgg cttgtggtga cacttcacct
    16741 tgcggtgaga gcgcatcaat aatcttcggc aattgctctg ccagtaaact ggaagctgta
    16801 ctggtatcca cgccaagttt ttgcccgaga tcggacaccg catttgtgcc gagcgccgat
    16861 tccagttgct cgccactaac cgattgattg ccctgttgat tactcagcca ggttgagaga
    16921 atggccccta agccgccact ttgcagtttt tccagcagca cctgaatgcc gccctgctcc
    16981 tcaacccaac ttaaaatagc ctgatatttc cccgcatcgc ctttcagaaa ggcaccgaca
    17041 acttcatcaa aaagccccat gataatcacc tgtaaagcgt tacgtgttga cccaaaaagt
    17101 atagatttgc gggatgataa ttgcggattg cagaaataaa aagggcggag atgatctccg
    17161 cccttttctt atagcttctt gccggatgcg gcgtgaacgc cttatccggc ctacaaaatc
    17221 atgaaaattc aatacattgc aagattttcg taggcctgat aagcgtagcg catcaggcac
    17281 gctcgcatgg ttagcgccat taaatatcga tattcgccgc tttcagggcg ttctcttcaa
    17341 taaacgcacg gcgcggttca acggcgtcgc ccatcagcgt ggtgaacaac tggtcggcag
    17401 caatcgcatc tttaacggta acgcgcagca tacgacggct ttccgggtcc atggtggttt
    17461 cccacagctg ttccgggttc atctcgccca gacctttata acgctggatg gagaggccgc
    17521 gacgggactc tttcaccagc cagtccagcg cctgctcgaa gctggctacc ggctgacgac
    17581 gctcgccacg ttcgataaac gcatcttctt ccagcaagcc acgcagtttc tcacccagcg
    17641 tgcagatacg acgatattcg ccaccggtga taaactcgtg atccagcgga tagtcagtat
    17701 ccacaccgtg ggtacgcacg cgaacaatcg gttcaaacag gttttgttcg gcattggtgt
    17761 ggacatcaaa cttccactgg ctgccgtgct gttctttgtc gttcagttcg ctgaccagcg
    17821 cgttcaccca gcgggtaacg gtctgctcat cagagaggtc ggcttccgtc agcgtcggct
    17881 gatagataag ctctttcagc attgctttcg gataacgacg ctccatgcgg ttgatcattt
    17941 tctgcgtcgc gttgtactca gacaccaatt tctctaacgc ttcgccagcc aatgccggtg
    18001 cgctggcgtt ggtgtgcagc gttgcgccat ccagcgcgat agagatctgg tactgatcca
    18061 tcgcttcgtc gtctttaatg tactgttctt gcttgccttt cttcactttg tacagcggcg
    18121 gctgagcgat gtagacgtga ccgcgttcaa cgatttccgg catctgacga tagaaaaagg
    18181 tcaacagcag cgtacgaatg tgcgagccgt cgacgtccgc atcggtcatg atgatgatgc
    18241 tgtgataacg cagtttgtcc gggttgtact cgtcacgacc gataccacag ccaagcgcgg
    18301 tgataagcgt cgccacttcc tgagaagaga gcatcttatc gaagcgcgct ttctcaacgt
    18361 tgaggatttt acccttcagc ggcagaatcg cctggttctt gcggttacgc ccctgcttcg
    18421 cagagccgcc cgcggagtcc ccttccacca ggtacagttc ggaaagcgcc ggatcgcgtt
    18481 cctggcagtc tgccagtttg cccggcaggc ccgctaagtc gagcgcacct ttacggcggg
    18541 tcatttcacg cgcgcgacgc gccgcttcac gggcacgggc agcatcgata attttgccaa
    18601 ccacgatttt cgcgtcggtt gggttttcca gcaggtattc tgccagcagt tcgttcatct
    18661 gctgttcaac cgctgatttc acctcagaag aaaccagttt gtctttggtc tgggaggaga
    18721 atttcgggtc cggcactttc acggaaacga ccgcaatcag gccttcacgc gcatcgtcac
    18781 cggtggcgct aactttggct tttttgctgt agccttcttt gtccatgtag gcgttcaggg
    18841 tacgggtcat cgccgcacgg aagcctgcca ggtgagtacc gccgtcacgc tgcggaatgt
    18901 tgttggtaaa gcagtagatg ttttcctgga agccatcgtt ccactgcaac gccacttcga
    18961 cgccaatacc gtctttttca gtggagaagt agaagatatt cgggtggatc ggcgttttgt
    19021 tcttgttcag atattcaacg aacgccttga tgccgccttc atagtggaag tggtcttctt
    19081 tgccgtcgcg cttgtcgcgc agacgaatgg aaacgccgga gttgaggaac gacaactcac
    19141 gcagacgttt cgccagaatt tcatattcga actcggtcac attggtgaag gtttcgaggc
    19201 tgggccagaa acgcaccatg gtgccggttt tttcagtctc gccggtaacc gccaacggtg
    19261 cctgcggtac accgtgttcg tagatctgac ggtgaatttt accctcgcgc tggataacca
    19321 gctccagttt ttgcgacagg gcgtttacta ccgaaacacc aacgccgtgc agaccgccgg
    19381 acactttata ggagttatcg tcaaatttac cgcctgcgtg cagaacggtc atgatcactt
    19441 ccgccgccga tacgccctct tccgggtgaa taccggtcgg aatgccgcgc ccgtcatcct
    19501 gtacagagac agagttatcg gcgtgaatgg tgacgataat ttctttacag tgacccgcga
    19561 gcgcttcgtc gatagcgtta tctaccacct cgaataccat gtggtgcaga ccggtgccgt
    19621 catccgtgtc gccgatatac atacccgggc gcttacgcac cgcatccagc cctttcagga
    19681 ctttgatact ggaggagtca taagaattcg acatcaacgt ttctcgctca tttatacttg
    19741 ggttaatccg ttattttacc cttttccacg gtaaacatct tcgaattttc gtccgacatg
    19801 tctataacgt gttcagcact gatcgcgctg acaaagacct gtgattgcgt cgcttttaag
    19861 cggctggcaa gcagcccgcg acgctcatca tcaagctcag aggcaaaatc atctatcagg
    19921 tagagacacc gccgcccgct ttcacgggtg aggaactctc cttgcgccag acgtaaggcg
    19981 cacatcaaca gcttaagctg cccacgcgat aaggtatctt ccaccggcgc accgtcggcg
    20041 cgaatgcgta agtccgcttt atgcgggcca tgcgcggtgt aggttagctg gcgatcgcgt
    20101 tcaaaattac gttccagcac ctcagcatat tctgtctctt tctcccagcc gcgctggaaa
    20161 gagaaagtca gagaaaactc agggagaaat tgcttacaag tatcggccat atcagccgcg
    20221 ataccggcgc tatactccgc gcgccaggtg ctgatttgct ccgccagcgg gatcagctct
    20281 ttatcccacg ggcgtagctg ttcgtaacgt gtcacctggc gcagcgccgc attgcgctgc
    20341 ttgagcaatc gcttgagatt gctccaggcg gtgaaaaatc cgggttcgtt gtgaaagcat
    20401 ccccagtcga ggaatgctct tctgtatttg gggccgccgt tgagtaaagt aaacccttct
    20461 ggcgttatca actgcattgg catcaggtgc gccagttccg cgaccttatg cccgtctgta
    20521 ccgtcgatgc ggactttgct gtcgccctgt ttgtctttgg ttaagccaat cgctgtctcg
    20581 cgctcttcgc cctgtaatcg cccgtggaga acaaacgcct cctgctcatg gcgaatgacg
    20641 cgaccaatct gcaaactgcg aaacgcccga ccatggccga gcgtatagat ggcttccagc
    20701 acgctggttt tgccactgcc gttggcacct accagaaagt taaagccggg agataaagcg
    20761 agatccgcgg tttcaatgtt gcggaaatcg cggatcaaca agcgggtgag ggacattaca
    20821 gtctcattgg catgacaaca taggcagccg actgtgatgc ggcatcttca atctgcacgc
    20881 tggaaaccga atcggtcagc atcatgcgga cgttttcaca tttcagcgcg ttcagaacat
    20941 ccagcacgta gctgacgttg aagccgattt ccatctccgc accgctatag gtaacgtcga
    21001 ggatctcttc cgcttcttcc tgttccgggt tgttggcggt gattttcagc tggttttcgc
    21061 tgacataaag acgtacgccg cggaatttct cgttagagag aatcgccgcg cgagcaaacg
    21121 cctgcttgag cagatcgcag ccagcttcca gatgtttgtc cgggttcttc ggcagaacgc
    21181 ggcgataatc cgggaagcga ccatccacca gtttggaggt gaagataaag tcgccaacgt
    21241 gggcgcgaat gttgttgctg ccaatctgta cgcgcagcgg attgtcgccg ccgtcgagca
    21301 tacgcatcag ttcaatcacg cctttacgcg gcacgatcac cgaatggctt ggcaaagatt
    21361 gaccaattgg cattgaacag actgccagac ggtggccgtc ggtcgccacg gtgcgcagtt
    21421 cttcgccttc ggtttcaaac agcataccgt ttaagtaata gcgaacgtcc tgatgcgcca
    21481 tagaaaactg ggtcgcttca atcagacgct tcatcgtcgc ctgcggcagg gtaaattcga
    21541 cttcgctctg ccagtcatcg aggttcggga aatccgccgc tggcagggta gacagcgaaa
    21601 aacggctacg cccggagcgc accagcatcc gttcaccttc cagctgtacg gcaatttccg
    21661 cgccttcagg cagaccacgg cagatatcaa agaatttgcg cgccggaacg gtcgtcgctc
    21721 ctggctcgtg tggctgaacc agcgcaacgc gtgccaccat ttccatctcg agatcagtac
    21781 cggtcagcga caacgtaccg tcagcaacct gtaacagcag attaccgaga atcggtagcg
    21841 taggacgacc acctaacggc ccgctcacct gttgtagcgg ttttaataaa tgctcacgtt
    21901 ctacggtaaa tttcataggt ttacgatgac aatgttctga ttaaatttga aaaatcttct
    21961 ttgatatcgt ggctctcttc acgcaactgc tcgatcttac ggcaggcatg aagcaccgtc
    22021 gtgtggtcac gaccaccaaa cgcatcgcca atctccggca gactgtggtt agtcagctct
    22081 ttcgccagcg ccatcgccat ctggcgcgga cgcgccaccg agcgggatcg acgcttggaa
    22141 aggagatccg cgactttgat cttgtagtac tccgccaccg tcttctgaat attgtcgatg
    22201 gtgaccagtt tttcctgcaa tgccagcaag tcgcgcagtg cctcacgcac gaagtcgatg
    22261 gtgatcgccc gtccggtaaa gttggcgtta gcaataacgc ggttcagcgc cccttccagc
    22321 tcacgtacgt tagatcgtag acgcttggcg ataaagaacg ccacttcgcc tggcaaacga
    22381 atgtcatttt cgtcggcctt tttcatcagg atcgccacgc gggtttccag ctctggcggt
    22441 tcgatcgcca cagtcagtcc ccaaccgaag cgggatttca aacgatcctc aacgccgttg
    22501 atctctttcg gatagcgatc cgaggtgaga atgatctgtt gattaccttc cagcagggcg
    22561 ttgaaggtgt ggaaaaactc ttcctgagat cgttctttat tagcaaaaaa ctgaatatcg
    22621 tcgatcagca gtgcatctac ggaacggtag tagcgtttaa actcttcgat cgcgttgttt
    22681 tgcagggctt taaccatgtc ctgaacaaag cgctcggagt gcatataaac cactttggca
    22741 ttcggcttgc gcgccataat gccgttaccc accgcatgca gcaggtgagt tttacccaga
    22801 cccgtgccgc cataaaggaa caatgggtta taggcaccgc cagggttatc cgccacctgg
    22861 cgagccgccg cgcgcgccag ttggttagat ttaccttcaa cgaagttatc aaacgtgtgt
    22921 ttgacgttta cgttagaacg ataggtcggt tctgccgggg ccgggacgtt atcccaacct
    22981 gagcgcgtag aaggcgcagc acgttgcggc tgcgtttgcg ccacctgtgc aggggccgcg
    23041 acgttgctcg tcactgccgc ttgtggcgtt tgcgtcaccg gtttggtgcc gacttcaaaa
    23101 cgcagctgtg gggcatccgc tccgcagaaa ctggttagca gtccattgat attattaagg
    23161 tacttgtccc gtacccaatc gaggacaaaa cggtttggcg cgtacagggc cagcgtgtta
    23221 tcgctcagtt ccgcctgcaa tgggcgtatc cacatactga attctgtggc tggtaactca
    23281 tcctgcaatc gggcaagaca ctgctgccaa agcgaaagtg acacggcgga ctccactcga
    23341 acaaaagtcg ataatgacta aggctgaaac attcatgatt gttgacgtac gtcgaaaaga
    23401 ccctgcttga gggtgacgca cgaaccgctg tctgcggtta tatgcccgat caagatcctg
    23461 caaaacgatc gggaccgcgg atcatagcct aaactgcgca agagatcttc tgtttctcac
    23521 agattttccc gatttatcca caggactttc cagacctcgc taagtgtaaa cgatcctgcc
    23581 gcgaggcggg cacgatttac gccgcatatt ggaaaattta atgaccatag acaaaaattg
    23641 gcttaatcga tctaataaag atctaggacg atccttgcgc tttacccatc agcccgtata
    23701 atcctccacc cggcgcgcca tgctggtttt cactggtgtg aggtcgtaca ttttccctgc
    23761 gaaaaggtgc ggaaaagcgc ggtaaataag gaaagagaat tgactccgga gtgtacaatt
    23821 attacaatcc ggcctcttta atcacccatg gcttcggtgt ccatcgtttc atttttcggc
    23881 ggatatccaa taaagccatt gaatttattc aagtttaggt agaaatcgcc atgaaacgca
    23941 cttttcaacc gtctgtactg aagcgcaacc gttctcacgg cttccgtgct cgtatggcta
    24001 ctaaaaatgg tcgtcaggtt ctggcacgtc gtcgtgctaa aggccgcgct cgtctgaccg
    24061 tttctaagta ataaagctaa cccctgagtg gttaagctcg catttcccag ggagttacgc
    24121 ttgttaactc ccagtcaatt cacattcgtc ttccagcagc cacaacgggc tggcacgccg
    24181 caaattacca ttctcggccg cctgaattcg ctggggcatc cccgtatcgg tcttacagtc
    24241 gccaagaaaa acgttcgacg cgcccatgaa cgcaatcgga ttaaacgtct gacgcgtgaa
    24301 agcttccgtc tgcgccaaca tgaactcccg gctatggatt tcgtggtggt ggcgaaaaaa
    24361 ggggttgccg acctcgataa ccgtgctctc tcggaagcgt tggaaaaatt atggcgccgc
    24421 cactgtcgcc tggctcgcgg gtcctgatag ccctcattcg ggtctatcaa cgcctgatta
    24481 gtccgctact cgggccgcat tgtcgtttca ctccaacctg ttcaagctac ggaattgagg
    24541 cattgcgcag gtttggagtg ataaaaggca gttggttgac ggtgaaacgc gtattaaaat
    24601 gccacccttt acaccctggt ggtgacgatc ccgtcccgcc cggaccattt gataccagag
    24661 aacactaacg atggattcgc aacgcaatct tttagtcatc gctttgctgt tcgtgtcttt
    24721 catgatctgg caagcctggg agcaggataa aaacccgcaa cctcaggccc aacagaccac
    24781 gcagacaacg accaccgcag cgggtagcgc cgccgaccag ggcgtaccgg ccagtggcca
    24841 ggggaaactg atctcggtta agaccgacgt gcttgatctg accatcaaca cccgtggtgg
    24901 tgatgttgag caagctctgc tgcctgctta cccgaaagag ctgaactcta cccagccgtt
    24961 ccagctgctg gaaacttcac cgcagtttat ttatcaggca cagagcggtc tgaccggtcg
    25021 tgatggcccg gataacccgg ctaacggccc gcgtccgctg tataacgttg aaaaagacgc
    25081 ttatgtgctg gctgaaggtc aaaacgaact gcaggtgccg atgacgtata ccgacgcggc
    25141 aggcaacacg tttaccaaaa cgtttgtcct gaaacgtggt gattacgctg tcaacgtcaa
    25201 ctacaacgtg cagaacgctg gcgagaaacc gctggaaatc tccacctttg gtcagttgaa
    25261 gcaatccatc actctgccac cgcatctcga taccggaagc agcaacttcg cactgcacac
    25321 cttccgcggc gcggcgtact ccacgcctga cgagaagtac gagaaataca agttcgatac
    25381 cattgccgat aacgaaaacc tgaacatctc ttcgaaaggt ggttgggtgg caatgctgca
    25441 acagtatttc gcgacggcgt ggatcccgca taacgacggt accaacaact tctataccgc
    25501 taatctgggt aacggcatcg ccgctatcgg ctataaatct cagccggtac tggttcagcc
    25561 tggtcagact ggcgcgatga acagcaccct gtgggttggc ccggaaatcc aggacaaaat
    25621 ggcagctgtt gctccgcacc tggatctgac cgttgattac ggttggttgt ggttcatctc
    25681 tcagccgctg ttcaaactgc tgaaatggat ccatagcttt gtgggtaact ggggcttctc
    25741 cattatcatc atcaccttta tcgttcgtgg catcatgtac ccgctgacca aagcgcagta
    25801 cacctccatg gcgaagatgc gtatgctgca gccgaagatt caggcaatgc gtgagcgtct
    25861 gggcgatgac aaacagcgta tcagccagga aatgatggcg ctgtacaaag ctgagaaggt
    25921 taacccgctg ggcggctgct tcccgctgct gatccagatg ccaatcttcc tggcgttgta
    25981 ctacatgctg atgggttccg ttgaactgcg tcaggcaccg tttgcactgt ggatccacga
    26041 cctgtcggca caggacccgt actacatcct gccgatcctg atgggcgtaa cgatgttctt
    26101 cattcagaag atgtcgccga ccacagtgac cgacccgatg cagcagaaga tcatgacctt
    26161 tatgccggtc atcttcaccg tgttcttcct gtggttcccg tcaggtctgg tgctgtacta
    26221 tatcgtcagc aacctggtaa ccattattca gcagcagctg atttaccgtg gtctggaaaa
    26281 acgtggcctg catagccgcg agaagaaaaa atcctgattc ggtgagtttt cgctaaaata
    26341 agggcggtca gttgaccgcc ttttttcttt tcgtagggtg gataagcacc gcgcatccgc
    26401 cacacaaagc aacaggaaca tcatgagcga taatgacact atcgtagccc aggccacgcc
    26461 tccgggacgt ggcggcgttg gcatcctgcg catctccggc ctaaaagccc gtgaagttgc
    26521 cgaaaccgtg ctgggtaaac tgcctaagcc gcgctacgcc gattatcttc cgtttaaaga
    26581 cgccgacggc agcgtgctcg atcaggggat tgcgctatgg ttccctggcc cgaactcgtt
    26641 caccggcgaa gatgtgctgg aactgcaggg tcatggcggt ccggtgatcc tcgacctgct
    26701 gttaaaacgc attctgacca ttcccggcct gcggattgct cgccctggtg agttttccga
    26761 acgcgcgttt cttaacgata aactcgactt agcccaggcc gaggcgattg ccgatcttat
    26821 cgacgccagt tcggaacagg cggcccgttc ggcacttaac tcgctgcaag gcgcattctc
    26881 cgcacgggtc aaccatctgg tggaagccct cacccacttg cgcatttacg tcgaagcggc
    26941 aattgatttc cctgatgaag agatcgattt cctctccgac ggcaaaattg aagcccagct
    27001 taatgacgtg attgccgatc tcgatgcagt gcgtgctgaa gcacgtcagg gtagtttgtt
    27061 gcgcgaaggg atgaaggtgg tgattgccgg acgtcctaac gccggtaaat cgagcctgtt
    27121 aaacgcgctg gcgggccgtg aagcagcaat cgtaactgat atcgccggaa ccacacgtga
    27181 cgtgctgcgt gagcatatcc acattgacgg aatgccgctg catatcatcg ataccgctgg
    27241 gctacgtgaa gccagtgacg aagtggaacg tattggtatc gagcgcgcgt ggcaggaaat
    27301 tgaacaggcc gaccgcgtgc tgtttatggt cgatggcacc acaacagacg ccgtggatcc
    27361 ggcagagatc tggccggaat ttatcgcccg tctgccagcg aaactaccga tcaccgtggt
    27421 gcgcaataaa gccgatatca ccggcgaaac gctgggaatg agtgaagtga acggtcacgc
    27481 gttaattcgt ctctcggcaa ggacaggtga aggcgtggag gtgctgcgta accatctcaa
    27541 acagagcatg ggctttgaca ccaacatgga aggcggcttc ctggcgcgtc gtcgccacct
    27601 acaggcgctg gaacaggcag cggagcattt gcagcaaggt aaagcgcaac tgttaggtgc
    27661 ctgggcgggt gaactgctgg cggaagagtt gcgcctggca cagcagaact taagcgaaat
    27721 caccggggaa tttacctctg atgatttgct ggggcggatt ttctccagct tctgtattgg
    27781 taagtaaccg cgcttacgaa gccgtattct gactgtcaga tgcggcttcg cttcattgtt
    27841 agcactcctg ttattcctca accctttttt taaacattaa aattcttacg taatttataa
    27901 tctttaaaaa aagcatttaa tattgctccc cg
//
LOCUS       DAJTZL010000038        27502 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.038, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000038 DAJTZL010000000
VERSION     DAJTZL010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27502
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.038"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(36..119)
                     /locus_tag="OO544_001433"
                     /pseudo
     CDS             complement(36..119)
                     /locus_tag="OO544_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 domain-containing protein"
     gene            complement(237..1676)
                     /gene="rsmF"
                     /locus_tag="OO544_001434"
     CDS             complement(237..1676)
                     /gene="rsmF"
                     /locus_tag="OO544_001434"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HCR6767061.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQISARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNQEENEAVCLWLKETYPDAVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKD
                     REAGQIRQAAAGVGLNWDENLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALATPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTGNA"
     gene            complement(1757..4390)
                     /gene="yebT"
                     /locus_tag="OO544_001435"
     CDS             complement(1757..4390)
                     /gene="yebT"
                     /locus_tag="OO544_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HCR6767062.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVNPSVVTL
                     LRENTRIELLNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLHEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            complement(4359..5642)
                     /gene="yebS"
                     /locus_tag="OO544_001436"
     CDS             complement(4359..5642)
                     /gene="yebS"
                     /locus_tag="OO544_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HCR6767063.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDTITGAMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERYY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            5772..6269
                     /gene="msrC"
                     /locus_tag="OO544_001437"
     CDS             5772..6269
                     /gene="msrC"
                     /locus_tag="OO544_001437"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HCR6767064.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            6366..7064
                     /gene="proQ"
                     /locus_tag="OO544_001438"
     CDS             6366..7064
                     /gene="proQ"
                     /locus_tag="OO544_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HCR6767065.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDALRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            7084..9132
                     /gene="prc"
                     /locus_tag="OO544_001439"
     CDS             7084..9132
                     /gene="prc"
                     /locus_tag="OO544_001439"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HCR6767066.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLAGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FGPELLKEHNARIAKDPEFQNIMKDIARFNAMRDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            9324..10205
                     /gene="htpX"
                     /locus_tag="OO544_001440"
     CDS             9324..10205
                     /gene="htpX"
                     /locus_tag="OO544_001440"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HCR6767067.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(10251..11314)
                     /locus_tag="OO544_001441"
                     /pseudo
     CDS             complement(10251..11314)
                     /locus_tag="OO544_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            11491..12282
                     /gene="kdgR"
                     /locus_tag="OO544_001442"
     CDS             11491..12282
                     /gene="kdgR"
                     /locus_tag="OO544_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HCR6767068.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDLDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            complement(12425..12664)
                     /locus_tag="OO544_001443"
     CDS             complement(12425..12664)
                     /locus_tag="OO544_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6767069.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            12823..12966
                     /gene="mgrB"
                     /locus_tag="OO544_001444"
     CDS             12823..12966
                     /gene="mgrB"
                     /locus_tag="OO544_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HCR6767070.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            13041..13328
                     /locus_tag="OO544_001445"
     CDS             13041..13328
                     /locus_tag="OO544_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HCR6767071.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVDDEDIIRLVAER"
     gene            13998..14141
                     /locus_tag="OO544_001446"
     CDS             13998..14141
                     /locus_tag="OO544_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobF family protein"
                     /protein_id="HCR6767072.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            14154..14363
                     /gene="cspE"
                     /locus_tag="OO544_001447"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             14154..14363
                     /gene="cspE"
                     /locus_tag="OO544_001447"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HCR6767073.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            14529..15338
                     /gene="rlmA"
                     /locus_tag="OO544_001448"
     CDS             14529..15338
                     /gene="rlmA"
                     /locus_tag="OO544_001448"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HCR6767074.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLSVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDDKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTFCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFTWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(15335..15901)
                     /gene="mntP"
                     /locus_tag="OO544_001449"
     CDS             complement(15335..15901)
                     /gene="mntP"
                     /locus_tag="OO544_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HCR6767075.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
     gene            complement(16330..16788)
                     /locus_tag="OO544_001450"
     CDS             complement(16330..16788)
                     /locus_tag="OO544_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 domain-containing protein"
                     /protein_id="HCR6767076.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLVSTQ"
     gene            complement(16843..17694)
                     /gene="manZ"
                     /locus_tag="OO544_001451"
     CDS             complement(16843..17694)
                     /gene="manZ"
                     /locus_tag="OO544_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HCR6767077.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            complement(17707..18507)
                     /gene="manY"
                     /locus_tag="OO544_001452"
     CDS             complement(17707..18507)
                     /gene="manY"
                     /locus_tag="OO544_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HCR6767078.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            complement(18570..19541)
                     /gene="manX"
                     /locus_tag="OO544_001453"
     CDS             complement(18570..19541)
                     /gene="manX"
                     /locus_tag="OO544_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HCR6767079.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            20004..21554
                     /gene="yoaE"
                     /locus_tag="OO544_001454"
     CDS             20004..21554
                     /gene="yoaE"
                     /locus_tag="OO544_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HCR6767080.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPIPE
                     GAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLFPV
                     CRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRARGS
                     FVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQQA
                     LDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIVKE
                     QPAHDEDE"
     gene            complement(21558..23156)
                     /locus_tag="OO544_001455"
     CDS             complement(21558..23156)
                     /locus_tag="OO544_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCR6767081.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVMEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQRFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDMLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMPLR"
     gene            complement(23287..24651)
                     /gene="sdaA"
                     /locus_tag="OO544_001456"
     CDS             complement(23287..24651)
                     /gene="sdaA"
                     /locus_tag="OO544_001456"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HCR6767082.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            complement(24835..25413)
                     /locus_tag="OO544_001457"
     CDS             complement(24835..25413)
                     /locus_tag="OO544_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HCR6767083.1"
                     /translation="MEYRSLMLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            complement(25417..26778)
                     /gene="pabB"
                     /locus_tag="OO544_001458"
     CDS             complement(25417..26778)
                     /gene="pabB"
                     /locus_tag="OO544_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HCR6767084.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITAQLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            26852..27031
                     /locus_tag="OO544_001459"
     CDS             26852..27031
                     /locus_tag="OO544_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HCR6767085.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
     gene            complement(27151..27474)
                     /locus_tag="OO544_001460"
     CDS             complement(27151..27474)
                     /locus_tag="OO544_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001442732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HCR6767086.1"
                     /translation="MVNFKDKNMPAVIDKALDFIGAMDVSAPTPSSMNESTAKGIFKY
                     LKELGVPASAADITARADQEGWNPGFTEKMVGWAKKMESGERSVIKNPEYFSTYMQEE
                     LKALV"
BASE COUNT         7080 a         6775 c         6943 g         6704 t
ORIGIN      
        1 cataaaacac tatcaataag ttggagtcat tacctggtta acgttcgatc acccggcgat
       61 tcaccgtgta attcgccgtc gtcgatttca aaagcgccta tgcgcacact ggttttcatc
      121 ttcgtgctct ccatttagct gccggttgtg gtacgaccag tccgatcgca ccatttttca
      181 gcctagttga ggcagccgaa tgtgccagtc aaaaagtgcg ctttttttgt gcgctgtcag
      241 gcgttaccgg taaaaagttt cccatcgcgc accagttcac gcggatagct gtttttcaat
      301 cgcgaaccaa tccgtttggc taaaccaatc ggttgatgct ggaaagtaac caacacgtca
      361 tccgccactg gcgcggcttg cgggtaaaca tcgcgcccgc gataccactc ctccgcttcc
      421 tgcggtgtca gttcaaaagc gttcacattg tcgggggtgg caagggcaat aactgcttca
      481 tgctgccagc gataaccttt gttgtgcgtt tcggcaagtt taatccccaa gcgagaaaat
      541 cggactttac cgatcagggc ttcaatgccc accgggaaca accacagttc tttgtcacgc
      601 tgccagaggc gcaggttttc atcccagttt aagccaacac ctgcagccgc ctgacgaatt
      661 tgtccagctt ctcgatcttt cactgggctg aacggaaaat taccgacttt gtatttgggg
      721 gcgggtaagg cgggaatcgc ctgagtttta cgcagacgag caacgaagaa gccttcgcag
      781 tcgtaaattt gtgggaaaac atgcaaaaag ccttcctcgg tcagcgcctt gtttgcacca
      841 gggaagagat caccaagcgg taaaaactct actgcgtcgg ggtaagtctc tttcagccac
      901 aggcaaacgg cttcgttttc ttcctggttt aaggtacagg tcgagtaaac cagcgtaccg
      961 ccagggcgta atgcatgaaa ggcgctgtcg atcagttccc gttgggtagc tgcgatttcc
     1021 tgattgcttt ctggtgacca gttttttagc gcatcgggat ctttacgcac cacgccttcg
     1081 ccggagcagg gagcgtccag caaaatggca tcaaacattt ctggcactgc cgcaccaaac
     1141 acgcggccat caaaatgtgt gagcgcaaca ttactgatac cacagcggct gatattggca
     1201 tgtaacactt ttacccgact ggcggaaaac tcattggcaa gaattgcccc ttcgttattc
     1261 atccgcgcgg aaatttgcgt cgttttggag ccaggcgcgg cagcgacatc catcacccgc
     1321 tgtggtgcat taccgtcagc aaacaaggcg gcgacgggca acattgaact ggcttcctga
     1381 atataaaaca ggccacttaa atgctcggcg gtactaccca atggcaatgc atcttcattg
     1441 tcgcgttcaa tccagaaacc ttcttcacac cacggaattg gcgtaagcgt ccagccataa
     1501 ggagcggtta attgcaggaa atcagcaacg gagattttca gcgtattaac gcgaatgctg
     1561 cggcgcaacg ggcgctgaca ggcggcaaga aaatcatcaa aagagagcgt cgaaggcatc
     1621 gcttcgcgca tttgcgtcag aaaggcgtcc gggaaataaa cggtgtgttg ggccacgagc
     1681 atataccact ggcaaaaaaa acaggcgcgc agtttagcat aaacgctccg gcgcaggcac
     1741 gccggagcag tgggcattat ttgggaagcg cagtacccca ttcacgccac tctttcggtt
     1801 cactttcctg caacaggaag tgcttgcctt cctgggcttt cggtgccagt ggcgtacccg
     1861 gaggcgtggc gaaggcgatg ccgccacgga taaactggtt aaaggtgccg gttttcacta
     1921 cgccgcccgt cagaccaaag tccagactgt aacccgatgc caaccagaag acggaattgt
     1981 tacgcaccag gtgttgatag cgtttactga tgcgcatcgc aatcatcacg cgatctgaca
     2041 atgtccccag cgtcattccg gtaaccgtac ctacttccag accacggaac agcacaggcg
     2101 taccaatacc taacgaaccg gcttccggtg cttcgacaat aatgcttaag ccatccaggt
     2161 aacgcgaatc agtaatagta gcttcttgca actcaaagtc acggcgagga ttaccccgac
     2221 ctggttcgac attgatatac ggctggagga tagtatctag atgctcaacg cccgcggctg
     2281 aaatttgcgg tgtgaccacc gagaagcgcg taccgccgcg ggcgaaggtc tggacatact
     2341 ccggatagag caccgccttt gcctgtactt cattgcgcgc ggtaatcaga tccagcgtct
     2401 ggatttgccc gatatcaata ccgagatagc gaattggcat cccgaccgcc agttttccgg
     2461 catcgaaagc gtgaagcgta atctgcccac caaccgcacg ggccgctgtt tcggaagcat
     2521 acagaattcg tttgtcgcct ttacgctgac tggcgctggc accgctgagg ttatcgaagc
     2581 taatggctcc ctttaatgct ctggagagcg gggatgcctg tacggtcagg ccactaccat
     2641 tcagctgaac tttcgccccg ccttctgccc agaacacgct attgctggtc agaaggttgc
     2701 gatactccgg cttaatatgc agatcgatat caaacgcgtt agctcgcgga cgcacggtaa
     2761 tcacttcacc aacttcaaat ttacggtaca gcactaccga tcctgcctgc acatccggca
     2821 gcgtctctgc actcaaactc acggttgtgg tgggtaaatc gctaaggctg ttctccagcg
     2881 ctttttccag attggcatac agtggatagc tggctttcat ctcaccttta tcgcctggca
     2941 gaatacgtat cccgccgtta atccattctg aggcgctggc accaagaaac tcaacgccat
     3001 ccagccccac cttcacgtcg acacggctgt tgacgacaaa tttgctatcg ccttttacca
     3061 gttcgcgatg ctgaggctcg atggcgacgg taaaggtaac gcctttgctg gtgagtttac
     3121 gatcaataac ctggcctacc tgcacgccgt gaagaatgag cggctgacca gcatcaatac
     3181 cgtaactttc cggcgcggtc agggtcagcg tcagaacatc aggttcatgc agcagtgctt
     3241 tttcgcctgg cacaacaacg aactctttgc gtggctcgcc atcgccgggt accaactcga
     3301 aggttttgcc agtcagcagg gcgctgagat tggcatcgct aagggataat ttcggattaa
     3361 gtaattcgat gcgggtattt tcacgaagca gggtaacgac gctgggatta actgtcattt
     3421 ccccagtgac tttaccacca ggatttaaat ccagtttagt tagctgtccg acttccagtc
     3481 cctgatacat taatggcgtc gagtcggcgg ttaatccggc cccactcggc agttccagtt
     3541 tgattattac gccacgctgg ctatgggcga ggtcttcata cagaccaaag gtatcttccg
     3601 cctcggcagg tttcgactct tcaggtgaat cgaaggcaat cgcaccgtta accagtgccg
     3661 ccagactttc cagtttcacc ttcgcaccac tgatactgac gttggcatca acgccggaaa
     3721 cgttccagaa acggctacct tttttcacta ggtcggtaaa acgccgctcg atcaggacat
     3781 caatcaccac gccttgcttg ttgggattga tggcatagtc gtagactttt cccacaggga
     3841 tcttgcggaa atagaccaat gaaccgctgt tcagcgaacc gagatcgggg gcttgcaggt
     3901 ggatcatcag atcgccattg tccagtcgat atttcggttg ggtatcgagt gcgacaaagt
     3961 gatcctgctc tttaccttta cctggcatca tgccgatata gttaccaccg acgagggcgt
     4021 ccagcccgga gacacctgcc aacgatgctt ttggcgtcac cagccagaac tgagtctctt
     4081 cgcgcagcgc atctttcata tcggacttga tgctgacctt gacttcaatc ttacgaagat
     4141 cgtcgctgag gctgatatcc tgcactgttc cgacttcaac gccctgataa cgaacaggcg
     4201 tacggcccgg gacaatacca tccgccgaca taaagtcgat ggtgacggta ttaccccggt
     4261 cctgataact gtcccaaatc agccaaccgg caatcattag cgcgatgaaa ggcagcagcc
     4321 agaaaggtga gatacggcgt ttatttttaa tctgcgcttc agtcgtcgaa gcgggcgttt
     4381 cctgactcat gtgcatccca aagtaagcgg ctgtccagcc attccacagc aagaatagtc
     4441 aaaattacgg ctgcgccaaa ataaaacgca gccggtccca tagtaaaagc gaggatctga
     4501 tcgcgattaa tcagcgacat ggttaaagat atgacaaaca ggtcgagcat cgaccagcgt
     4561 ccaatccagg tcaccatccg cagtaacaga atgcgtgtgc gtaaaccttg ctggcattta
     4621 aaatgaatgc tcaacagtaa agtgaacatg acgatcactt tagtaaacgg taccagaata
     4681 ctggcgataa acacgattcc ggcgaccgca atgttgctac ttgccagcga cataatcccg
     4741 gaaagaatcg tatcttcctg ccggccacca ttcagataaa tgatagaaat aggcaatagg
     4801 ttggctggta acaacaaaac gattgacgct aacagcgccg cccagcattt ttgcaaacta
     4861 tgacgacgac gcaggcgtag cgggatatgg caacgcgggc agcgaccacg ctgatcagga
     4921 tagccggtaa aatggcaccc aagacagaca cgaagtttct cgtccctacg cgtagcgggg
     4981 cgctgcggat aatatcgctc ccacagctcc tcaacattaa gatgtgacaa cgtcaccgtc
     5041 gttaaaatca ccaacgccac aaaagagaac aagccgacac ccgcctggat atgggcataa
     5101 tcctgtacct ttatagaagc aacgccaatg ccgaccaggt agatatccag cattacccac
     5161 tctttaagac gctcaagcat cagcagtacc ggacgtaaat tcattcccag tcggttacca
     5221 aaccataaat atgctatgga ggacaccaga atgagggggg caccaataac gcagaaaaag
     5281 accatcgccc ccgttatcgt atcgccctgt ttggtcattt gccagatgcc ttgcatgacg
     5341 ttggcgtcga tacgaatacc taacagccag atatgcaaca gcggttcgcc ccaggcaaac
     5401 ggcatcagca acagcatggt gaaagccatt gccgccaggc gcgttagcga ccagtcgcgc
     5461 ccgtcgcgaa tttttgcctg acagcgcgga caataggcac tctgatgaga atttatctcg
     5521 ggcaggctaa acagcatgtc acattgcggg caacgttggt aatcaccacg cggcagttcc
     5581 tcgccgattg ccctcactgt tatctttttt gtcggcgtaa tttgtggtgt gttaagagcc
     5641 atgacgtgcc aaatgtaata agaattaaca ttaatcttaa cccatgatgg gaatgatctt
     5701 gagcattaac gcatttaatg cttattttaa taggctggca gttccaggta agacaaacaa
     5761 gtgattatat aatgaacaaa acagaatttt acgcggattt aaatcgcgac tttaacgcgc
     5821 tgatggcggg agaaaccagt tttctggcaa cgcttgcgaa caccagtgcg ttgttatacg
     5881 agcgtctcac tgacgtaaac tgggcaggtt tttatttgct tgaggacgat acactggtac
     5941 tcggaccatt tcagggcaaa attgcctgtg tccggatacc cgtcgggcgc ggggtgtgcg
     6001 gcactgcggt tgcccgcaat caagtgcagc gtatcgagga tgttcacgcg tttgacgggc
     6061 atattgcctg tgatgcggcg agtaattctg aaattgttct gccgctggtg gtgaaaaatc
     6121 agattattgg tgttcttgac atcgatagta ccgtcttcgg tcgctttaca gacgaggacg
     6181 agcaaggctt acgtcagctt gtggcacagc ttgaaaaagt gcttgcaacg acggattaca
     6241 aaaaattctt tgcgagcgtc gcaggataat caacggataa cgtagcattt actggtagcg
     6301 tcattataat gacgcctgtt catgcctgcg cttgttggct acgtccgttg taatcaggaa
     6361 atttcatgga aaatcaacct aagttgaata gcagtaaaga agtaatcgcg tttctggccg
     6421 aacgttttcc ccactgtttc agtgcggaag gtgaagcgcg tccgctgaaa atcggtattt
     6481 ttcaggattt ggtcgatcgt gttgccgggg aaatgaacct gagcaaaacg caattacgat
     6541 ccgctttacg tctctacact tcgagctggc gttatcttta cggtgttaaa cccggcgcaa
     6601 cgcgtgtcga tcttgacggc aacccatgcg gtgagctgga cgagcaacat gtagagcatg
     6661 ctcgcaagca gcttgaagaa gcgaaagcgc gtgttcaggc acagcgtgct gaacagcaag
     6721 cgaaaaaacg cgaagctgcc gcagctgctg gtgagaaaga agacgcactg cgccgcgaac
     6781 gcaagccacg tccgaccacg ccacgccgca aagaaggcgc tgaacgtaaa cctcgtgcgc
     6841 aaaagccggt agagaaagcg ccaaaaacag taaaagcacc tcgcgaagaa cagcacaccc
     6901 cggtttctga catttcagct ctgactgtcg gacaagccct gaaggtgaaa gcgggtcaaa
     6961 acgcgatgga tgccaccgta ttagaaatca ccaaagatgg cgtccgcgtc cagctgaatt
     7021 cgggtatgtc tttgattgtg cgcgcagaac acctggtgtt ctgaaacgga ggccgggcca
     7081 ggcatgaaca tgttttttag gcttaccgcg ttagctggcc tgcttgcaat agcaggccag
     7141 accttcgctg tagaagatat cacgcgtgct gatcaaattc cggtattaaa ggaagagacg
     7201 cagcatgcga cggtaagtga gcgcgtaacg tcgcgcttca cccgttctca ttatcgccag
     7261 ttcgacctcg atcaggcatt ttcggccaaa atctttgacc gctacctgaa tctgctcgat
     7321 tacagccaca acgtgctgct ggcaagcgat gttgaacagt tcgcgaaaaa gaaaaccgag
     7381 ttaggcgatg aactgcgttc aggcaaactc gacgttttct acgatctcta caatctggcg
     7441 caaaagcgcc gttttgagcg ttaccagtac gctttgtcgg tactggaaaa gccgatggat
     7501 ttcaccggca acgacactta taaccttgac cgcagcaaag cgccctggcc gaaaaacgag
     7561 gctgagttga acgcgctgtg ggacagtaaa gtcaaattcg acgagttaag cctgaagctg
     7621 gcaggaaaaa cggataaaga aattcgtgaa accctgacac gccgctacaa atttgccatt
     7681 cgtcgtctgg cgcaaaccaa cagcgaagat gttttctcgc tggcaatgac ggcgtttgcg
     7741 cgtgaaatcg acccgcatac caactatctt tccccgcgta ataccgaaca gttcaacact
     7801 gaaatgagtt tgtcgctgga aggtattggc gcagtgctgc aaatggatga tgactacacc
     7861 gttatcaatt cgatggtggc aggtggtccg gcagcgaaga gtaaagctat cagcgttggt
     7921 gacaaaattg tcggtgttgg tcaaacaggc aagccgatgg ttgacgtgat tggctggcgt
     7981 cttgatgatg tggttgcctt aattaaaggg ccgaagggca gtaaagttcg tctggaaatt
     8041 ttacctgctg gtaaagggac caagacccgc actgtaacat tgacccgtga acgtattcgt
     8101 ctcgaagacc gcgcggttaa aatgtcggtg aagaccgtcg gtaaagagaa agtcggcgtg
     8161 ctggatattc cgggcttcta tgtgggtttg acagacgatg tcaaagttca actgcagaaa
     8221 ctggaaaaac agaatgtcag cagtgtgatc atcgacctgc gtagcaatgg cggtggggcg
     8281 ttgaccgaag cggtatcgct ctcaggtctg tttattcctg cgggtcccat tgttcaggtc
     8341 cgcgataaca acggcaaggt tcgtgaagac agcgataccg acgggcaggt gttctataaa
     8401 ggcccgctgg tggtactggt tgaccgtttc agtgcttcgg cttcagaaat ctttgccgcg
     8461 gcaatgcagg attacggtcg tgcgctggtt gtgggtgaac cgacgtttgg taaaggcacc
     8521 gttcagcaat accgttcatt gaaccgtatt tacgatcaga tgttacgtcc tgaatggcca
     8581 gcgctgggtt ctgtgcagta cacgatccag aaattctatc gcgttaacgg cggcagtacg
     8641 caacgtaaag gcgtaacgcc agacatcatc atgccgacgg gtaatgaaga aacggaaacg
     8701 ggtgagaaat tcgaagataa cgcgctgccg tgggatagca ttgatgccgc gacttatgtg
     8761 aaatcaggcg atttaacggc ctttgggccg gagctgctga aggaacataa tgcgcgtatc
     8821 gcgaaagatc ctgagttcca gaacatcatg aaggatatcg cgcgcttcaa cgctatgagg
     8881 gacaagcgca atatcgtttc tctgaactac gctgtgcgtg agaaagagaa taatgaagat
     8941 gatgcgacgc gtctggcgcg tttgaacgaa cgctttaaac gcgaaggtaa accggagttg
     9001 aagaagctgg atgatctacc gaaagattac caggagccgg atccttatct ggatgagacg
     9061 gtgaatatcg cactcgatct ggcgaagctt gaaaaagcca gacccgcgga acaacccgct
     9121 cccgtcaagt aatatcaatc aggcacaaga aattgtgcct gattttttaa cagcggcaag
     9181 atgccgtaaa tcagatgcta caaaatgtaa agttgtgtct ttctggtgac ttacgcacta
     9241 tccagacttg aaaatagtcg cgtaacccat acgatgtggg tatcgcatat tgcgttttgt
     9301 taaactgagg taaaaagaaa attatgatgc gaatcgcgct cttcctgcta acgaacctgg
     9361 ccgtaatggt cgttttcggg ctggtactga gcctgacagg gatacagtcg agcagcgttc
     9421 aggggctgat gatcatggcc ttgctgttcg gttttggtgg ttccttcgtt tcgcttctga
     9481 tgtccaaatg gatggcatta cgatctgttg gcggggaagt gatcgagcaa ccgcgtaacg
     9541 aaagggaacg ttggctggtc aatactgtag caacccaggc tcgtcaggca gggatcgcta
     9601 tgccgcaagt ggctatctac catgcgccgg acatcaacgc ttttgcaacc ggtgcgcgcc
     9661 gtgatgcctc tctggttgct gtcagcaccg gtttgctgca gaacatgagc ccggatgaag
     9721 ccgaggcggt aattgctcac gaaatcagcc acatcgccaa tggtgatatg gtcaccatga
     9781 cgctgattca gggcgtggtg aacaccttcg ttatctttat ttcccgtatt ctggcgcagc
     9841 ttgccgcggg ttttatgggc ggaaatcgtg atgaaggtga agagagcaac ggcaacccgc
     9901 tgatctactt tgcggttgca acggttctgg aactggtgtt tggtattctg gcgagcatta
     9961 tcaccatgtg gttctcgcgt catcgtgaat tccatgctga tgccggttcg gcaaaactgg
    10021 ttggtcgcga gaaaatgatt gccgcgctgc agcgcctgaa aaccagctat gaaccgcaag
    10081 aagcaaccag catgatggct ttctgcatta acggtaagtc gaaatcgctc agtgagttgt
    10141 tcatgactca cccgccgctg gataaacgaa ttgaagctct gcgtacgggt gaatacctga
    10201 agtaatatga agtaaatact aaaaagcgcg tcctgatcga cgcgcttttt ttatgccttg
    10261 gatcgtggct gagtgatacg taaaccactg acacaggcag caatcactgc cagaatagcc
    10321 gcagccatca gcgaaacgtg cgtaccatta tcaccgaact gatttagcat cagcgccacc
    10381 agtgccgcgc cgctactctg acccagtaga cgagccgttc ctaacatgcc actggctcca
    10441 ccgctacgtt cgcgaggagc ggaggtaata atggtgtggt tattgggtga ctggaacaag
    10501 ccaaatccag cgccacataa gatcatcggc cagataatat tgatatccgc aggtgacgcg
    10561 ggcagcagaa ccaggggaaa aagccccgca gccatgatga acaaccctag agcacccagc
    10621 aatcctgcat gtacgcgttc aatcaaatag cctgccaacg gagccatcac catcgttgct
    10681 aacggccacg gtgtcagaag taaacctgtt tcgacttcac tacgcccgag cacggtttgc
    10741 aggtaaaagg gcagggaaac cattgccagc atttgtgcgc agaaagagca aacagatgtg
    10801 caaatagaaa gtgaaaacag cgggatacgc agtaaatcca ccggtagcag cggtacggga
    10861 agagaaagct ggcggcgaat aaagaaaata ccaacaacaa ccattaccac cagttccgca
    10921 gcaattaacg tcagcgattg cccctgagcg aaaccactca acgcagtgat aagcaggccg
    10981 aaggttaacg cgttcatcac ggcgctgggc aggtcgaaac ggggtttact ggcgcgagaa
    11041 ccattgggtg gcagaaaacg catcgccaga agcagggcga taatacctaa cggtacgttg
    11101 attaaaaata accatttcca ggatgcgatg gagaggattg ctgcagcaat tgtcggcccg
    11161 gcagcagaag agacggcaac aatatggcga ttgttggcag ggcgacgttg gcgattgcgc
    11221 cgtcaaggac agccatcgaa atgccaatca caatggttaa tatcgcaccg tatcgctggg
    11281 gcaatggcag gccgtcggcc tgaacttttg gcataggaat tttatatctt tggtgaataa
    11341 tcaacagggg tatgattcta gcactgatat tcacggtgga tgtcgcagaa ttgtaactaa
    11401 ccggcaaaag atggattgcg accaccctgg tgcgaaatta taataaaaaa cagttctgat
    11461 ttttataaaa cactcgcaat gaggtgataa atggctaacg cagatctgga taaacagcct
    11521 gattctgtat cttccgtgct aaaagttttt ggcattttgc aggcgctggg tgaagagcgc
    11581 gaaataggga taaccgagct gtcgcagcgc gtcatgatgt caaaaagcac cgtttatcgc
    11641 tttttacaga ccatgaaaac cttaggttat gtggcgcagg aaggggagtc ggagaaatat
    11701 tccctgaccc tgaaattgtt tgaactgggc gctcgcgcgt tacaaaacgt cgatttaatt
    11761 cgtagcgcag atatccagat gcgtgagatc tcccgcctga ccaaagaaac tatccacctc
    11821 ggcgcactgg acgaagacag tattgtttac attcacaaaa ttgactctat gtacaatttg
    11881 cgcatgtatt cacggattgg gcgtcgtaat ccgctgtaca gcaccgcgat tggtaaggta
    11941 ctgctggcat ggcgcgatct cgatgaagtg aagcaaattc ttgagggcgt ggagtataaa
    12001 cgcagtaccg agcggaccat caccagtaca gaagcgttat tacccgttct ggaccaggtg
    12061 cgcgagcagg ggtatggcga agataacgaa gagcaggaag aagggctgcg atgcattgcg
    12121 gtaccggtat ttgatcgctt tggcgtggtc attgccggtt tgagcatctc cttcccgacg
    12181 ttgcgtttct ctgaagagcg tttacaggaa tatgtcgcaa tgttgcatac cgcagcgcgc
    12241 aaaatttctg cccaaatggg ttatcacgac tatccgttct gatgagagta agaacctgtc
    12301 ggaatatcaa acagacaggt tctttattta gcatgagaaa aataaagttg aaggtgcgtt
    12361 atattaaacg cgcttgctat aagaatattt tactcatgag tgagaatctg gttgtttatt
    12421 acccttaacc attatcgacc acgatattgc ttttacgtaa cagtgggcaa tctgttatcc
    12481 ccaagaaacc acttttagtg tgcaagtatt gtaccgtgct ggtgcctctg gcagtcagat
    12541 aggtacattg caaacctaat cctgcggcat tctctttgct tccaatcaaa acgccatatc
    12601 cgctgagtaa taatcctatc cataccagtg ctatcagcat aactgtgcga atgatgaatc
    12661 gcattacaac ctcttctctt tttatgttcg cttaatcgta gcggcaatat gcgctgaagc
    12721 aagctactca ttccgaaaaa gcacgaatat cgacatagtt aggcgctgtt taactaacgc
    12781 atgctagttt aatgacataa ggtaggtgaa gcggagattg gagtgaaaaa gtttcgatgg
    12841 gtcgttctgg ttgtcgtggt gttggcttgc ttgctgcttt gggcgcaggt attcaacatg
    12901 atgtgcgatc aggatgtaca atttttcagc ggaatttgtg ccattaacca gtttatcccg
    12961 tggtgatatc attttttcag cactagattt tcttcctctc cagcagtggt agaatagcgc
    13021 ctctactcag aggtggtgaa atgaacgaag ttgtaaattc aggcgtgatg aacattgcgt
    13081 ctttggttgt atcggtggtg gttcttctta tcgggctcat cttgtggttt tttatcaatc
    13141 gtgccagttc acggactaac gaacagattg aactgcttga ggcgttgctg gatcagcaaa
    13201 aacgtcaaaa tgcactgtta cgtcgtttgt gcgaagcaaa cgaaccagag aaagcagata
    13261 aaaagaccat tgagagtcaa aaatcggttg atgacgaaga tattattcgc ctggtcgccg
    13321 aacgataatc cttcctcaat cttgagtaac cccgtcgttg aagggttact caattcatcc
    13381 tgattatacg cactgtcatg gtgcaactgc ttcaaattaa aactctggtc tgacttcaaa
    13441 taattatcat attatttatt tgcgtaacgg gatatcagaa aaagatattt tcctcattaa
    13501 tttattagga tgtttacatc ggatttgtga ttaagcgtgg tattatttat tagcgaaacg
    13561 tttctctctt gatttttttg ctcattcatc aatttttctt attttaaatt ttcaatccct
    13621 ttggggattg acttctcttt agggtaattg atagccgtta accgactgtt ttatgagaaa
    13681 aagtgatata actttttatt cattgcatag caaaaaatgt gatattgcac gcactatgta
    13741 ataacttctc ccactggcct ggaacaactg aacttattga actatgttag aaaatacgcc
    13801 agtttaagta tctgcctgaa ctggcaaggt taagcacaat gatatatcgg cgcgtattcc
    13861 gttgcataag tgtgcaaaaa aagtggaaga cgtatcgaga tttgtgcgtc tgatcgagac
    13921 atgtttaaaa atggcttgcc ataattaacg ttgtatgtga taacagattt cgggttaaac
    13981 gaggtacagt tctgtttatg tgtggcattt tcagtaaaga agtcctgagt aaacacgttg
    14041 acgttgaata ccgcttctct gccgagcctt atattggtgc ctcatgcagt aatgtgtcag
    14101 ttttatctat gttatgcctg cgggcgaaga aaacaatcta aggaattttt caaatggcaa
    14161 agattaaagg tcaggttaag tggttcaacg agtctaaagg ttttggcttc attactccgg
    14221 ctgatggcag caaagacgtg ttcgtacact tctccgctat ccagggtaat ggcttcaaaa
    14281 ctctggctga aggtcagaac gttgagttcg aaattcagga cggccagaaa ggtccggcag
    14341 ctgttaacgt aacagctatc tgatcgaatc cactgatctg aagtgtgaat acgcttcaat
    14401 ctcgctataa agcctcgtcg aatgcgaggc tttttactat gctttatctt cgctcctggc
    14461 gttcggatat ttgcccgccg cgtgattcgc gttacacttg cggcctttag tatcctgccg
    14521 gagttgtcat gtctttttcc tgtccacttt gccatcagcc tctttcgcgt gaaaaaaaca
    14581 gctatatctg tccccagcga catcagtttg atatggcgaa agaagggtat gtcaatctgc
    14641 tgtccgttca gcataaacgg tctcgtgatc cgggcgacag cgcggaaatg atgcaagcac
    14701 gccgcgcatt cttagatgcc ggacattatc agccgctgcg tgatgcaatt gtcgcccaac
    14761 tgagggaacg gcttgatgat aaggccacgg cggtgctgga tattggctgt ggtgaagggt
    14821 attacacaca cgcatttgcc gatgcgttgc ccgaaatcac cacgtttggt ctggatgttt
    14881 cgaaggtagc gataaaagcg gcggcgaaac gctatccgca ggtcactttt tgtgtcgctt
    14941 ccagccaccg tttgccgttt tccgatacca gtatggacgc cataatacgt atttacgcgc
    15001 cgtgtaaagc agaagaatta gcacgagtag tgaagcccgg cggctgggtc attactgcca
    15061 cgccgggacc gcgacatttg atggagctga aggggctgat ttacaatgaa gtacatcttc
    15121 atgcacctca tgcagaacaa ctggaaggtt ttacattaca gcagagtgcg gagttgtgtt
    15181 atccgatgcg tcttcgcggt gatgaagccg tcgcattatt gcagatgacg ccgtttacct
    15241 ggcgtgcgaa gccagaagtc tggcaaacac tggcagcaaa agaagtgttc gactgccaga
    15301 cggactttaa tattcacctc tggcagcgtt cttattaacc gtggaagtgc gtccagagga
    15361 tctggacgcc gatgccgatc agtaccagcc cgccgagaat ttccgctttt ttcccaataa
    15421 ttgagccgat aaagcgacca accatcatcc ctaatgttga cataatcaag gttgcacaac
    15481 caatggccaa tgcggtcgcg ataatgttga cctgcaggaa agcaagacca acacccacag
    15541 ccatggcatc caggctggtg gcaatcgcgg tggttaccag tagccagaaa ccgtgtcgac
    15601 ggcgcggctc ttcatcttca tcatctgcgc cacgaaaacc ctcaataatc attcgcccgc
    15661 cgaggaatat cagcagcaca aacgcaatcc agtggttcca ttcaaggaca aaccggctgg
    15721 ctaacatgcc cattccccag ccgatcagcg gcgtcagggt ttcgacggca ccaaaaataa
    15781 ggccggttcg caatgcttca gaaaatttcg gtttatggag ggtggcacct ttaccgattg
    15841 atgcagcaaa tgcatccatc gacataccaa acgcaagaag aacagtagca gtgatattca
    15901 tgacaatgtc ctgaccgggg tatccataaa acacatcatc gcccccagca aacatgcata
    15961 catgaacaac aagccgtgat ggttttcgcg ttcatgcagg catagcgatt gatgtgcata
    16021 tggtctcgcc tgactgaaag tattcagtcc gtacgtgcca cgttttgcaa cgagtatgtt
    16081 gacacgtaca tttccggaat ctggaaatcg gctactcccc aaatgagggc gcaaccttaa
    16141 catattttga gaatataaaa caactgttgg aatatattat ttcattgctt tattgataac
    16201 gattttcatt tggatttata tatcaatgaa acgttaaaat aaataacaaa taagggaaga
    16261 ggtaatatag cctaagctat gtctgattat taaaaataca ctaaacatag ctttggctaa
    16321 attcataact tattgagttg aaacgagaag cttataaatt ttttccagat cgtcgatatt
    16381 tcgaacgcgg attaacagcc gacgctgttc taattgcatc accaacacgc catcttccga
    16441 caagttcatc gctttgattc ggctatattc aatccagaca ttggcgaaga aaaaaccttt
    16501 ttgtttaaag atgatcttcg gaacgcggat ccagaatata taaaaaccca tcagagccag
    16561 tgcgcttaat aaccatgtcg ttattaacgc accatgattc gtgacgttgt tatagataag
    16621 aatgacaatc aatccgacga agataacgct atcgatgcga ccacgccgga gcaaaggaat
    16681 tgccagcagg gtggggccgt tacggcgggg catgatgaac tgatcgtaga tcgcgaaggc
    16741 caggagtgcg gcgatgaaaa gaatcagtac caggtccgtg attgtcatta atcctccaga
    16801 taaaaaaacg gggccaaaag gccccggtag tgtacaacag tcttacagtc ccagcaggcc
    16861 gcaagcgtaa ccagcgatac cgatgacgaa gaagccaacg atgatccaca gcgggttaac
    16921 ttttttgcgc agtagccaca tacaagcaaa ggtcagcagc agtggtacca ggcctggcat
    16981 taactggtcc agaatagtct ggacagtagt aacgtgttct ttgcccgtct ggtcagtaat
    17041 gcgagagaca accagcggga tgttgacatg tgtccacttg ttaaccaatg cccccatgac
    17101 aaacaggccg aggatagacg ccccttccgt cagtttttgc aggaagccac cacccatatc
    17161 tttaacgata tcgatacctt tggagtaacc atacgctacg ccgtagtaac gggttgccag
    17221 acgcaccagg ttaaacagga tgaagaacag cagcggacct aacaggctgc cgctcatcgc
    17281 gataccggca cccagtgctg caaataccgg acgtacggtt ccccagaaga tcgggtcgcc
    17341 tacaccagcc agcggcccca tcaaaccgac tttgataccg ttgatagcac cgtcgtcgat
    17401 ctctgcgcca ttagcacgct gttcttccag cgccagggtt acgccgagaa tcggcgcagc
    17461 cacgaacggc tgggtgttaa agaactccag gtgacggcga atagcttgtt tacgagcttc
    17521 gttgttctca gggtagaggc gacgaattgc cggtaccata gagaagcaga aacccagtgc
    17581 ctgcatacgt tcgaagttcc atgaaccctg gaagaggtta gaacgcagga agacgccacg
    17641 aatatcactt tgagtgagtt ttttctcggt ggtagtttga gttgtatcaa ccatttcgct
    17701 cacctgttag tccagttcgt tatcgagatc gttgttacca gctgcctgag caggcgcacc
    17761 ggctacgcgg ttgtatttcg ggctaagttg gatgtagagc actgccataa cagtaccaat
    17821 cacacccaga gcaaccaggt taaagttggt gaatgctgcg gttacgaagc cgaggtagaa
    17881 gaacggcatc aggtagccag cacgcatcat gttgataacc atcgcataac caaccaccac
    17941 gatcatgcca ccggcgatat tcagaccatt ggtcaccact tccggaatcg cattcagcat
    18001 gttctgtact tcgctggtac caacagacag cgcaacgatg acggccggaa tagccacacg
    18061 cattgcttgc aggaacagag aagaaacgtg gatccaggaa atcgctgtca ggttgccgtt
    18121 atcagcagcc ttatccgcag cgtgctggaa agcaacggta atagtacgaa cgatgatggt
    18181 cagtacctgg cccgcagcgg ccagagggat tgccagtgcg atacctgcac caatgctctg
    18241 atgacctgcg ataaccagaa tggtagaaat gatagaagcc agagcggcgt caggcgcaac
    18301 tgcagcaccg atgttcatcc agcccagcgc gatcatttcc agcgtaccac cgataataat
    18361 accggttttc atatccccaa gaacgatacc caccagggta cacgcgatta gcggacggtg
    18421 aaactgaaat tcatcgagga ttgatcccat acctgcgata caggctacga taaataccag
    18481 cacaatttga agagtggtaa tctccattgt acttctcctg tttacgtaag acttaagtgt
    18541 gaaaactgag tgataatcaa cacaatacgt tacttatcga ttttgctgat cagatccatc
    18601 attttcagtt tcggatcggt ggaaacctta cggacttcca gctcaatacc gcgcgcattc
    18661 agtttcttga acgcctcgat atctttttca tcaaccgaaa ccgcgttatt cacctgggtt
    18721 ttaccctgac ggaatgccat accaccgacg ttaacagagg tgattttcac gccgccttca
    18781 acgagacgct ctacatctgt tgggttggta aataacagca ttacgcgttc gccagcatat
    18841 ttcgggttgt tgtagacgcg aatcattttg gcaacatcaa ctacgtgtgc tgttacgccc
    18901 ggaggtgcaa cctgggtgag cagtgtctta cgaacggtat ccgcagccac ttcatcacta
    18961 acaacaataa tacgggagac attggtttct ttggtccagc gggtggcgac ctgaccgtga
    19021 atcagacggt cgtcgatacg cgcaaggcca ataaccatgt agtcgtttgg ccccattggt
    19081 tttgccggag ttggagccgc ttttggtgct gcggcagcgg gtgctggcgc ggctttttca
    19141 accggtttgg ctttcagtgc tttcacgcct tcacggcctg tttctactgc cagcgcaacc
    19201 agttcatcaa agcttgggtc atcatcacga gccattaacg tttccacgag cattggaatg
    19261 ttaacgcctg caatgacttc ataatgctct ttgtcgacga caatgcggct ggcagcattg
    19321 aacgggctgc ctccccatgt atcaacgaga aacagcacgc ctttagtggt gtcgagtttt
    19381 gccaactgag cgttgtactt ttcaatcagc gtttcggcat tttcacctgg aacgaaatcg
    19441 atccagccga cgttttcctg ctcgcctaac agcatttctg ccgttttaag caactgctct
    19501 gcagcccaac catgtgtgcc tataacaata gcaatggtca cttgctacct cctttattat
    19561 cgttaacacc tcaacgtgcc agatgtattt ttgaatcgtc gtccacaatc gaatcgattc
    19621 agataagggc tacaaccaaa aacccctgcg tttcgcgatt tattttagat atcgaaaaaa
    19681 ttattttatg tgatgaagat ccgtaattta actttcgatt agcagaaatt tcgaaaggta
    19741 aaatatcctt gtcacattcg tttgcaaagg aaggtaaatc tttgccaaat cagaggcgtc
    19801 tctgatatgt ttaactcccg tttaacaacc atggagtata gggcagtagc ccgcagtatg
    19861 gatcgtcacc gacgtcattt cagcatcagg ccttttaacg cctgcctttc tggcactcta
    19921 tgccgcacct ttcgtttgca ttttgtcgtt acgcctgcat tatttctggc gtcgaatagc
    19981 tattccttaa gcaggagctt gtcatggaat tcttaatgga cccctcaatt tgggcggggc
    20041 tactcacgct tgttgttctc gaaattgtgc tgggtatcga taacctggtc ttcatcgcca
    20101 ttcttgctga caaactgccg ccaaaacaac gcgataaagc gcgtttgctg gggttatcac
    20161 tggcgctgat tatgcgtctg gggctgctgt cgctgatttc atggatggtc acgctgacca
    20221 aaccgctatt taccgtcatg gatttctcct tctccggacg cgacctgatt atgttgttcg
    20281 gggggatatt cttgctgttc aaagcaacaa ccgaactgca tgaacggctg gaaaaccgcg
    20341 atcatgattc cggccacggt aaaggctacg ccagtttctg ggtggtcgtc acacagattg
    20401 tcatccttga cgccgtcttc tcgttggatg cggtaattac tgcagtaggg atggttaacc
    20461 atctgccggt gatgatggcg gcggtagtga ttgcgatggc ggttatgttg ctggcatcca
    20521 aaccgctgac gcgattcgtt aaccagcacc ccacggtggt ggtgctctgt ctgagcttcc
    20581 tgttaatgat tggtctgagt ctggtggcag aaggtttcgg tttccacatt ccgaaaggtt
    20641 acctgtatgc cgcgattggc ttctcgatca tcatcgaagt gtttaaccag attgcgcgtc
    20701 gcaactttat tcgccaccag tcgactttgc cgctgcgagc gcgtactgcc gatgccatcc
    20761 tgcgtttgat gggcgggaaa cgtcaggcca atgttcagca cgatgccgat aacccgatgc
    20821 cgatcccgga aggtgcattt gccgaagaag aacgttacat gattaacggc gtactgacgc
    20881 ttgcgtcgcg ttctctgcgc gggatcatga cgccgcgcgg tgaaataagc tgggttgacg
    20941 ctaatctcgg ggtcgatgaa atccgcgagc aactgctctc ttcaccgcac agtctgttcc
    21001 cggtatgtcg cggtgaactg gatgaaatca tcggtattgt acgtgctaaa gaactgctgg
    21061 tggcgctgga agagggcgtt gatgtggcgg cgattgcttc ggcgtctccg gcgattatcg
    21121 tcccggaaac cctcgatccg atcaacctgt tgggcgtgct gcgtcgtgct cgcgggagct
    21181 ttgttatcgt gaccaacgag tttggtgtgg tacaaggtct ggtcacgccg ctggatgtgc
    21241 tggaagccat tgcgggtgaa ttcccggacg ctgacgaaac gccggaaatc attactgatg
    21301 gtgacggctg gctggtaaaa ggcggtacag atttgcatgc cttgcagcag gcgcttgatg
    21361 ttgagcacct tgccgatgac gatgatatcg cgacggtcgc gggcctcgtg atctcggcaa
    21421 atggtcacat tccccgtgtg ggcgatgtga ttgatgtagg gccactgcat atcaccatca
    21481 ttgaagccaa tgattatcgt gttgatctgg ttcgcattgt taaagagcaa ccggcgcacg
    21541 atgaagatga gtaagcatta acgtaacggc ataatgggcg tgatatgtcc attatgccgg
    21601 gcgggcggcg gttggctgcc cgccagccat ttgggaaaat cacgtagcgg catcggctgt
    21661 gcgtacaaat acccttgcaa aatatgcacg ccatgacggc gcaaatactt cgcctgttct
    21721 tgcgtttcca cgccctccgc caccagttca atattcagtc tttgccccag cgcgatgatg
    21781 atatcggtca ccgtcgagtt aaccgcgtca gaacctatag ctgcggtaaa tgacttatca
    21841 attttcagca tgtcaggacg taatgtttca agccaggaaa atgaactgtt gccggtgccg
    21901 aagtcatcaa tcgccagttt gacgttttta cgatgtagct cgcgggcaat ccgataatca
    21961 acatccagta aggcatcgcg ttcggtgatt tcgaggatca gttgctgaat cgggtgagcg
    22021 ctaaaccagt actgattgag atcttttatc agcacaccac ggcgaaaatg gctgggtgcg
    22081 acgttaatgc caacatgaaa ctgactactc atcgggaaaa catggcgctg acgaatggtt
    22141 tctgccatca catagcgggt cagtggcaca attaaatgat gttcttccgc gataggaata
    22201 aacacatccg gtgaaatcca gccctgacgc ggattgttcc agcgcagcaa aatctctaca
    22261 ccaatacatt gctggctgcg cgcattaagc aaaggctgac agaacaattc gaactcatgt
    22321 tgcgccaggc ccagattgat ttcgcgggaa aagctcatcc ggtaagcggt cgccagccag
    22381 gcgatgtagc ccaccagtaa acttagcagc accgccagcg gtaattgtgt tggaagatag
    22441 tgccatgcca gcgccgtagc ccccggacca ttaacgttaa tggtaaaagg aaagcgttgc
    22501 gaagaaacct gatagttttc attgtcttca ggttgcggaa gggaatctac cagcccatta
    22561 ccatagagca aatgccgttt gtccaccgtc aggctggcgc tactgatttg cggcagttgt
    22621 ggctcaagga gcattgccgt cagtaagtcg atgttaatca tctccatgac cccagcattg
    22681 ctactgcccg ctgcaggcgt ccattgaatc aaaaccggac tgcctttaat cagggcacgg
    22741 tcgatcgtca ggcgtaaaag tggttgcggt gcaggaagtt cagccagaat gtccacgacg
    22801 ggcacattgc gataaccaaa aatgctggag caatagagtg ttccgtcttg caccaggcca
    22861 atggatcgca cagtttggag ttttgccgcc tgtttacgta atggcaaatg ggcgacagag
    22921 cagggcagac caatcagcgg aagcaagact tcgctacctg cctgtagggg aagcagtact
    22981 ttatctaatt cctctacagc gtgattggcg aattgtacta cccgttgttg atttaagtta
    23041 cgctgtgaaa taaatcgcac gctcagggtc gaagcgagcg taacgagtgc gcaaatcgta
    23101 caaacaatca ttcgattacg gcgataggtt ttaatgatcc gttgtgcttt ttgcatgcgg
    23161 gccaccctgt aagcgtatgg cgacaaacgc ctgacgcaac agtggaagtg tagtggggaa
    23221 tattacagca gacgagaaag cgggtataaa ttcgcccatc cgttgcagat gggcgagtaa
    23281 gaagtattag tcacactgga ctttgattgc cagaccaccg cgtgaggttt cgcggtattt
    23341 ggcgttcatg tccttaccag tttcgtacat cgtttcgatg accttatcca gcgagacgcg
    23401 cggtgcactg gtgcggcgca gagccatccg tgcggcgtta atcgccttca cagaggcaat
    23461 ggcattacgc tcaatgcacg gcacctgaac ctgccctgca accgggtcgc aggttaaacc
    23521 aaggttgtgt tccatgccaa tttccgccgc cacgcaaacc tgttccgggc taccgcccag
    23581 cagttctgca agacccgcag cagccattga acaggcaaca cccacttcgc cctggcaacc
    23641 aacttccgca ccggaaatag aggcgttcat tttatacaat gcaccaatcg cgcccgctgc
    23701 cataaagtaa cgggtataga tgtccgggct gaccgattca ataaagtggt catagtaagc
    23761 cagcactgcc ggaacgatac cgcaggcacc gttggttggc gcagttacca cacgaccacc
    23821 ggcggcgttt tcttcgttaa ctgccagcgc aaacatgttt acccagtcaa tgacattcat
    23881 cggatcgtta gacagtttat cgctggaaac taacatccgg cgcagggcag acgcacgacg
    23941 tggcacgcgc agcgggcctg gcagtacacc ttcggtgttc atcccgcgat cgatacatgc
    24001 ctgcatggtt tgccagacat gcgcgaaata ctcgtcgatc tctttcttgc tgtgcagcgc
    24061 cagttcgttc tgcatagcga gaccagacag cgaatagccg gtttcattac agtacgcgag
    24121 cagttcggtg gcagatttga acggatacgg cacgctcact tcgttggcag catcctgacc
    24181 aaagtgttct tcatcgacga taaaaccgcc gccgatggaa taataagttt tgctgtagac
    24241 gacttcatcg ccgttatagg cgtggatttg cataccgttt tcatgcagcg gcaggttgcc
    24301 gttatgaaaa cgcatcccgt tgtcgcgcgg gaaatccact tcatgccgtc cctgtgccag
    24361 cagcagacgt tcgcgctctt ctacgtcgcg aataaaaccg ggaatactgt cgatatccac
    24421 ggtggcaggt tcgttacctg caagacccat aataatggcg atatcggtgt ggtggccttt
    24481 acccgtcagc gacagtgaac cataaacgtc cacggcaacg cgagtaacgc tatccagtaa
    24541 gcctttttcg accagatcat cgacgaactg tttacctgcc ttcataggcc ctacggtatg
    24601 ggaagatgag ggaccaatcc ccaccttaaa catgtcgaat agactaatca cgataatact
    24661 cctgacaagg tgactggact tccagtaacg aactaataac agcgcatagt gtaagaggga
    24721 acgcggcgac tggcttaact attcacatga attaaactaa tcaaaaaccg cgatttaact
    24781 aatattgcga tgattgtctc aagtagtgtt ttgtagatgt aaagataagt atagtcaggg
    24841 tttcacacca atttgcagcg ccagctcacg aattatgcct gcggtcattc cccatacaaa
    24901 atactgttcg taccaggaca gccatacccg atgtgaatcg ccacggcggt agatatctaa
    24961 agggtgataa cgacccagat gtaatgcctg ggcgagcggc atttcaaaca ccgccgagac
    25021 ttcatcttca ctggcgcgat acggcagatc gggcgggata atgccgacca ctggggttac
    25081 ctgatagcca gttacgctat cgacgggcgg cagcacgccg ataacttcaa cggcggaagg
    25141 cggtatagcg acctcttctt cagcttcgcg cagcgcggcg gcgataactg atgcgtccgt
    25201 gtcatcgact gcacctccag ggaatgccac ttgtccagcg tgtttacgca gatgaattga
    25261 acgctgagtc agcaacaacc ccggttgcgg tcgacggacg atggggatta acacggcagc
    25321 ctgacgatga tttagggttt cccggttaat ttgcgggcgc aaaagttgaa agcgcgataa
    25381 aaaatcatca agcatcaggc tacggtattc cacgtcttac ttctccagtt ggcgtaatat
    25441 cttattaact ttatcaaaag tttcctgata ttccgcttct tcctggctat cggcgacaat
    25501 tccaccgccc gcagagcagt atatttgtcc gttaatggca gtcagagtgc ggatagtaat
    25561 gctggtatcc atgttgccgc aaaagctcaa atagccaatg ctgccgcacc aggcattacg
    25621 tcgctgcggt tccagttcgt cgataatttc catagcccgt actttcggag ccccggttat
    25681 tgagccacca ggaaaagctg cgcgcagcag atcgctggcg tgtaactgtt ctggtagttg
    25741 tgccgttatg gtgctgacca gatgatgcac ggcagggaag ggttccacca cgaacagctc
    25801 tggtactttt accgaacctg ctacggcaac acgaccgata tcattacgca ttaaatcgac
    25861 aatcatcaga ttttcggcac gatctttcgc tgagttcgcc agtttttctg cttgtttgct
    25921 atcttcctga ggatcgggca ggcgtggtag cgtgccttta atagggcggg tctggatttc
    25981 actattatca caaagaataa accgctctgg cgaaaggctt aaaattgcac cctgttcaag
    26041 acgtaaaaaa gcgctaaatg gcgcgcggtt ggcctgatta agctgaagga atgcctgcca
    26101 ttcatcgcca gaataggtcg catgaaaacg ctgggcgaga tttacctgat agcaatcacc
    26161 gctgtgcaga tattcctgta cctggcgaaa tttttcgccg tactgctcgc gggtcatatt
    26221 ggattgccag tcggaagtaa gcgtgaaatc ttcctgcggc gagaattgct ggctttccag
    26281 ccaggcccga cgggcattga catcattatg actcagcaaa gaaactgtat gacgctggtg
    26341 gtcgacaatg agcgcccaat cgtagatacc cactgccata tccggcagaa cgatatcttg
    26401 ttccgcaatt tctggcagtg actcaaaacg gcggcccaga tcgtagccaa acaaccccag
    26461 tgcgccgccc tgaaatggca aatcttcgtt atgcgttggg cgaatgtctg cgcgatccag
    26521 cacctgctgg agcacctgta gcgggtcatc agtggtcgtt gtgcgttttt cgctttcact
    26581 aacaacggtt tctttaccta aagtggttaa agtgcaaatc ggatcggcga ccacaatatc
    26641 aaagcggcta tacggatgat cggcatagcc ggagtgtaaa agcatcgccc acggcaggtg
    26701 gcttaagcgg gagaaataaa attcagcggc gtcctgacgc cagggtaaag taatcacagc
    26761 gggagataac gtcttcatca gtcctgactc tactggctat gtgccgggta gcatgaaata
    26821 attagcgcca acaatttagc aggagttaac aatgtttgca ggtttacctt cactcaccca
    26881 tgaacagcag caaaaagctg tcgagcggat ccaggaactg atggcacaag ggatgagcag
    26941 cggccaggca atcgcgttgg tggcagaaga gctgcgtgcc aaccattccg gcgagcgaat
    27001 cgtggcgcgt tttgaggatg aagacgagta acacactaat ccgtattgat ttcgttatac
    27061 aagccgatga ttatttgatt gtgagaaaaa ggacgctttt tatatgtgat taaaagcgtc
    27121 ctgatccatt tatgctgatg taaattttac tcagaccagt gcttttagtt cttcctgcat
    27181 atatgttgaa aagtattcag gatttttaat cacagagcgt tcacctgact ccattttttt
    27241 tgcccatcca accatttttt ccgtaaaccc agggttccag ccttcctggt cagctcgcgc
    27301 cgtaatatca gcggcactcg caggtactcc cagttctttt aaatatttaa agattccctt
    27361 cgccgtgctt tcattcatcg aacttggtgt tggcgctgat acatccatgg caccaatgaa
    27421 atccagggct ttatctatta cagcaggcat attcttatcc ttaaaattaa ccatgttatc
    27481 gataaggcta tatatgacct ta
//
LOCUS       DAJTZL010000039        26772 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.039, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000039 DAJTZL010000000
VERSION     DAJTZL010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26772
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.039"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            110..382
                     /locus_tag="OO544_001461"
     CDS             110..382
                     /locus_tag="OO544_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid shock protein"
                     /protein_id="HCR6767087.1"
                     /translation="MGLSSAAFAAETATTPAPTATTTKAAPAKTTHHKKQHKAAPAQK
                     AQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHSHQQPAKPAAQPAA"
     gene            475..558
                     /locus_tag="OO544_001462"
     CDS             475..558
                     /locus_tag="OO544_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6767088.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            658..1479
                     /locus_tag="OO544_001463"
     CDS             658..1479
                     /locus_tag="OO544_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HCR6767089.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTNDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(1518..1847)
                     /gene="mdtI"
                     /locus_tag="OO544_001464"
     CDS             complement(1518..1847)
                     /gene="mdtI"
                     /locus_tag="OO544_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HCR6767090.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(1834..2199)
                     /gene="mdtJ"
                     /locus_tag="OO544_001465"
     CDS             complement(1834..2199)
                     /gene="mdtJ"
                     /locus_tag="OO544_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HCR6767091.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            2611..3645
                     /gene="tqsA"
                     /locus_tag="OO544_001466"
     CDS             2611..3645
                     /gene="tqsA"
                     /locus_tag="OO544_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HCR6767092.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLISVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            complement(3670..5058)
                     /gene="pntB"
                     /locus_tag="OO544_001467"
     CDS             complement(3670..5058)
                     /gene="pntB"
                     /locus_tag="OO544_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HCR6767093.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(5069..6601)
                     /gene="pntA"
                     /locus_tag="OO544_001468"
     CDS             complement(5069..6601)
                     /gene="pntA"
                     /locus_tag="OO544_001468"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HCR6767094.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            7125..8069
                     /locus_tag="OO544_001469"
     CDS             7125..8069
                     /locus_tag="OO544_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR6767095.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEILGVATTA
                     SPSSEVGRFFETQSSKGGRYTVMLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            8255..9637
                     /locus_tag="OO544_001470"
     CDS             8255..9637
                     /locus_tag="OO544_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR6767096.1"
                     /translation="MKKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            9674..10396
                     /gene="folM"
                     /locus_tag="OO544_001471"
     CDS             9674..10396
                     /gene="folM"
                     /locus_tag="OO544_001471"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HCR6767097.1"
                     /translation="MGKAQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLIKAGAQCIQADFSTNDGVMAFADEVLKSPHGLRAILHNASAWMAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHIAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(10393..10728)
                     /locus_tag="OO544_001472"
     CDS             complement(10393..10728)
                     /locus_tag="OO544_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HCR6767098.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVSCWGISAWV
                     LIICWIKLH"
     gene            10857..11576
                     /gene="rstA"
                     /locus_tag="OO544_001473"
     CDS             10857..11576
                     /gene="rstA"
                     /locus_tag="OO544_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HCR6767099.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            11580..12881
                     /gene="rstB"
                     /locus_tag="OO544_001474"
     CDS             11580..12881
                     /gene="rstB"
                     /locus_tag="OO544_001474"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HCR6767100.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALITFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLAQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            12957..13886
                     /gene="tus"
                     /locus_tag="OO544_001475"
     CDS             12957..13886
                     /gene="tus"
                     /locus_tag="OO544_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HCR6767101.1"
                     /translation="MARYDLVDRLNTTFRQMEQELAAFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(13883..15286)
                     /gene="fumC"
                     /locus_tag="OO544_001476"
     CDS             complement(13883..15286)
                     /gene="fumC"
                     /locus_tag="OO544_001476"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HCR6767102.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQVKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(15429..17075)
                     /gene="fumB"
                     /locus_tag="OO544_001477"
     CDS             complement(15429..17075)
                     /gene="fumB"
                     /locus_tag="OO544_001477"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HCR6767103.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGCDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            17274..18449
                     /gene="manA"
                     /locus_tag="OO544_001478"
     CDS             17274..18449
                     /gene="manA"
                     /locus_tag="OO544_001478"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416130.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HCR6767104.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     RNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPNAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            18550..20058
                     /locus_tag="OO544_001479"
     CDS             18550..20058
                     /locus_tag="OO544_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HCR6767105.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATEFMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYTNGQITLNGQKMPLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(20239..21549)
                     /gene="uidC"
                     /locus_tag="OO544_001480"
     CDS             complement(20239..21549)
                     /gene="uidC"
                     /locus_tag="OO544_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HCR6767106.1"
                     /translation="MRKIVAMAVICLTAASGLISAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKSGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVN
                     YRLTPQFTLTFAQIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLKSEITPERKTSGVSDC"
     gene            complement(21588..22961)
                     /gene="uidB"
                     /locus_tag="OO544_001481"
     CDS             complement(21588..22961)
                     /gene="uidB"
                     /locus_tag="OO544_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HCR6767107.1"
                     /translation="MNQQLSWRTIVGYSLGDVANNFAFAMGVLFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHSRKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQVQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKVQQQ
                     LISDITN"
     gene            complement(22958..24769)
                     /gene="uidA"
                     /locus_tag="OO544_001482"
     CDS             complement(22958..24769)
                     /gene="uidA"
                     /locus_tag="OO544_001482"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HCR6767108.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            complement(25158..25748)
                     /gene="uidR"
                     /locus_tag="OO544_001483"
     CDS             complement(25158..25748)
                     /gene="uidR"
                     /locus_tag="OO544_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HCR6767109.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIKGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTSMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            complement(25969..26736)
                     /gene="hdhA"
                     /locus_tag="OO544_001484"
     CDS             complement(25969..26736)
                     /gene="hdhA"
                     /locus_tag="OO544_001484"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005153726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HCR6767110.1"
                     /translation="MRHAPCAMRHAPCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFACRCDITSEQELSALADFAISKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRL
                     GQPQDIANAALFLCSPAASWVSGQILTVSGGGVQELN"
BASE COUNT         6716 a         6939 c         6435 g         6682 t
ORIGIN      
        1 ccattcccag ggatatagtt atttcaacgg ccccgcggtg gggttaaatg aaaaaacaaa
       61 ttgagggtat gacaatgaaa aagtattagc tctggttgtt gccgctgcta tgggtctgtc
      121 ttctgccgcc tttgctgcag agactgcgac cacacctgct ccgactgcga cgaccaccaa
      181 agcagcgccg gcgaaaacga cacatcataa aaaacagcat aaagcagcac ctgcccagaa
      241 agcgcaggcg gctaaaaagc atcataaaaa tacgaaagct gaacagaaag cccctgaaca
      301 aaaagcgcag gcagcgaaga aacacgccgg gaaacacagc catcagcaac cggcaaaacc
      361 tgctgcacaa cccgcagcgt aagttttcaa cagtaatgct ggcgcgcccc ctcgcgcctg
      421 aaaattacgg tgctaagcgg gtaacgttta gcaccgcctt tagcggaggg tagtatggtg
      481 ggccgttatc gctttgagtt cattcttatc atccttattt tatgcgcact gattgccgcc
      541 cgtttttatc tttcctgact gtagttatct gattttactc ccactttcat cccgtcccgt
      601 ctatagtatt tatgagggtt tgcttttaat aatcataatt acccaccaga gtgtgatatg
      661 cgtacaacca ttgctgtagt gttgggtgca attagtttga cgtctgcttt tgtgtttgca
      721 gataaaccag acgttgccaa atcggcaaac gatgaggtca gcaccctgtt ttttggtcat
      781 gatgatcgtg tgccagtgaa tgacacgacc caatcaccgt gggatgcggt tgggcaactg
      841 gaaacggcca gcggcaattt atgtacggcg acgctgattg cacccaatct ggcattaacg
      901 gcaggacact gtttattgac acctccaaag ggtaaagccg ataaagcagt ggcgctgcgt
      961 tttgtgtcaa ataaaggtct ttggcgctat gagatccacg acatagaagg ccgcgttgat
     1021 ccgacactgg gaaagcggtt aaaagcagat ggggatggtt ggattgtacc tcccgcagcc
     1081 gcgccgtggg acttcggatt gattgtgcta cgtaatcccc cttctggcat tacaccgttg
     1141 ccgttatttg agggagataa agccgcgctt actgccgcat taaaatcggc tggccgtaaa
     1201 gtaactcagg cgggataccc tgaagatcat ctcgatacgt tgtacagtca tcaaaactgt
     1261 gaagtgactg gctgggcgca aacgtcggtg atgtcacatc agtgcgatac cttgccgggt
     1321 gacagcggtt cgcctctgat gttgcatacc aatgacggct ggcaattaat tggggtgcaa
     1381 agttcggctc ctgccgcgaa agatcgctgg cgcgccgata accgggccat ttctgttacc
     1441 ggttttcgcg acaagctgga tcaactgtcg caaaaataat gttcaaacgc tgcccgactg
     1501 tgcgggcagc gtgttcatca ggcaagtttc accatgatca ttccagccaa cagcaagacc
     1561 aggccaatcc agcctttacg atttaaccgt tgaccaaaca agatccaacc tgcggctaac
     1621 gtggcggcaa taccaaaccc gccccacaat gcataagcga cagacaagtc gatcccttta
     1681 acggcctgag aaagcgcact aaaggcagcc agcaccgccg ccagggagag caagccaaat
     1741 attttgcgac gaaagccgtc agaaaatttc aaaaagacgt tagcaacgat ttccagcacg
     1801 attgccaatg ccagccaggc ggcgtgaacc cattcaaact gcgccatggt tcacctccag
     1861 ttcaggttta cgcgctttac gggtacctga ttttatcaac acaatcccgg cgaccagggt
     1921 ggttaacccg gcaattttca tcagcgataa actttcgtcg aataacaaaa cgctaaacaa
     1981 ggtaataaat aaaataccga taccttccca cagcgcataa gctacgccta aggcgatctt
     2041 tttaacggcg aaagagagaa atatatacga cagagaaatc atcaccagca ttaaaataaa
     2101 gccgccattt ccctcactga cgctcgccca tttcattgac agcgtaccgg taatttctgt
     2161 agcaatagcc aggcctaata aaatccaata aatatacatt gtccttctcc tgcaagagaa
     2221 ttattttaat tttcgcttaa ttcagcgaaa ccaaagtaaa gtggtcaagc cccgcgcata
     2281 gggcaagctt aggcagaaga aaggactaaa gcgcgttgcg ccagtgctgc tcacctacga
     2341 gcaagatagt ggatgaggta cgaagatggg taaatgttga aaataacgtc ctgaacaaat
     2401 tgtccataat attacaatta tccgcagtgt tgcttctcgt catcgcggat gataattgtc
     2461 ctcggtagtt gaacacgcct gatttgtatc atagcttaag aattaactca aaatattttc
     2521 acttctttac ctgagcggtt tgattttcgt tatgatgacg gagcgaaaaa gacattatta
     2581 ttagcaaagg aagaaaaaac ggggacaagc atggcaaagc cgatcatcac gctcaatggc
     2641 ctaaaaatcg tcattatgtt gggaatgctg gtcattattc tctgcggtat ccgttttgcc
     2701 gccgagatca tcgtgccgtt tattctcgca ttatttattg ctgttattct taacccgctg
     2761 gtgcaacaca tggtccgctg gcgagtgccg cgtgtactgg cggtgtcgat tttgatgacc
     2821 atcatcgtga tggcgatggt gttgctatta gcttatctgg gttccgcgct caacgagttg
     2881 acgcggacgt taccgcaata tcgcaactct attatgacgc cgctgcaagc tcttgagccg
     2941 ttgttgcaac gcgtagggat tgacgtctca gttgaccagc tggcgcatta cattgatccg
     3001 aacgcggcga tgacgttgct caccaactta ttgacgcagt tatctaatgc catgtcatca
     3061 atatttttat tgctgctgac ggtgctgttt atgctgctcg aagtgccaca attgcccgga
     3121 aaatttcagc aaatgatggc gcgtccggtt gaagggatgg cggcgattca acgtgcaatt
     3181 gacagtgttt ctcattatct ggtgctgaaa acagccatca gcatcatcac cggcctggtc
     3241 gcctgggcga tgctcgccgc actcgatgtt cgcttcgctt ttgtctgggg attgctggcc
     3301 tttgcgctta attacatccc taatattggt tcagttctcg cggcaatccc ccctatcgct
     3361 caggtactgg tgtttaatgg cttctacgaa gcgttgctgg tgctggcggg atatctgctg
     3421 attaatctgg tcttcggcaa tattctggag ccgcgcatca tgggacgtgg gctggggctt
     3481 tccacattgg tggtattttt gtcgttgatt ttttggggat ggttgttagg accggtgggt
     3541 atgctgattt ccgtgccgtt aacaattatt gtcaaaattg cgcttgaaca aacagcggga
     3601 ggtcaaagca tcgccgttct gttaagcgat ctcaataaag agtgacggcc tccgcagagg
     3661 ccgtcagggt tacagagctt tcaggattgc atccacgcta gctttggcgt caccaaacag
     3721 catgtgggtg ttttccttga agaacagcgg gttttgcaca ccagcatagc cagtgttcat
     3781 cgaacgttta aagacaatca cgttctgcgc tttccacact tccagcacag gcataccagc
     3841 aataggactc ttcggatcat cctgcgccgc cgggttaacc gtatcgttag caccaatcac
     3901 cagtacggta tcggtatcag caaagtcatc gttgatctcg tccatttcca gcacgatgtc
     3961 atacggtact tttgcttcag ccagcaatac gttcatatgt ccaggcaaac gccccgcaac
     4021 cgggtggata ccgaaacgca cgttgatacc acgagcgcgc agtttctcgg taatttcagc
     4081 gacaggatat tgcgcctgcg cgactgccat gccgtacccc ggagtaatga tcactgaatg
     4141 ggagtttttc agcagttccg ctgtctcttc tgcggtgatt tcgcggtgct cgcccacttc
     4201 ctgatcatcg ccagtagaag agccgtcggt gccgaaacca cccgcaataa cgctgataaa
     4261 ggaacggttc atcgccttac acataatgta agaaaggata gcccccgaag aaccgaccag
     4321 cgcaccggtc acaatcagca ggtcgttgct gagcataaag cccgcagccg cagccgccca
     4381 gccggagtac gagttcagca tcgacaccac cactggcata tctgcaccac cgatggaggc
     4441 gactaaatgc cagccgaata ccagcgcaat ggcggtcatt attagcaatg ccagcacttg
     4501 caggccgacg ctgtccgtgc gaacaaatac aatcagcagc aggaaggaaa cgaccagagc
     4561 cgccaggttc attttgtgac ggtttggcag catcaacggt ttagacgaaa tcttgccaca
     4621 cagtttgccg aacgccacca ccgaacccgt gaacgttacc gccccgatga agataccgag
     4681 gaacacttcc gtcaggtgaa tattgaccag aatcggtgcc attcccgcgt catgatgcag
     4741 atagctgtta aagccaacca gcactgccgc cagacccacg aagctgtgca ggatcgccac
     4801 cagttctggc atttcggtca tttcaacttt cttcgccaga cggataccaa ttgccccacc
     4861 aatgaccatc gccagcaaga tccagccaac attacccgta tccggtccaa aaatggttgc
     4921 gattaacgca atcgccatcc cggcgatacc gaagttgtta ccctggcgag acgtttcatg
     4981 tttcgaaaga ccggccagac tgaagataaa caggatcgcg gcaacaatgt atgcagctgt
     5041 aactaatcct ccagacatat gttacccctt aatttttgcg gaacattttc agcatgcgct
     5101 gagtcacggt gaagccaccg aaaatattaa tgctggctat aagcaccgcg ataaaactaa
     5161 ggaagctaac ccagccgccc tggccaatct gcaacagtgc tccgacaaca ataatccctg
     5221 aaatcgcgtt ggtgaccgac atcaacggtg tatgcagcgc gtgcgataca ttccacacca
     5281 cgtaataacc gacaacgcag gccagcgcga aaacggtgaa gtgcccaaga aactctttcg
     5341 gcgcaacgct tgccagccaa ccgaaaagga tgattgccag cgccatcaac gcgtatttac
     5401 gccacggtga gcaggcacat ttttcctcag ttttcacttc cggtgccgct ttttgtgccg
     5461 cctgcggctg agctgatacc tgaatcggcg gtgccggcca ggtaatttcg cccgcacgga
     5521 tcacggtcac gccgcgaatc accacatcat caaaatcaac agtgatattg ccgtctttct
     5581 ctttgcacaa cagtttcagc agattaacga ggttagtacc gtaaagctgc gaggattgcg
     5641 tcggcagacg gcccggaaga tcggtataac caatcacttt gacaccattt tccgtagtga
     5701 agatttcacc cggcacggtg tattcacagt tgccaccgtt ttgggctgcc aggtcgacaa
     5761 tcacactgcc cgccttcatg gagtcaacca tttcacgggt aattagcttc ggcgctggtt
     5821 tgcctggaat aagcgcggtg gtgacaatga tatcgacctc ttttgcctgg gcggcaaaga
     5881 gttccatttc cgctttaatg aacgcgtccg acatcacttt ggcatagcca tcgccactgc
     5941 ccgcttcctc tttaaaatcc agctcgagga attccgcgcc catactttga acttgttctt
     6001 tcacttccgg acgggtgtcg aatgcacgca caatcgcgcc gagactgttt gctgcgccaa
     6061 tggcggccag acctgcaaca cccgcaccaa tcaccatcac ttttgccggt ggcactttcc
     6121 cggccgcagt aatttgcccg gtaaagaagc gcccaaattc atgtgccgct tcaacaatgg
     6181 cgcgataacc ggcgatgttc gccatcgagc ttagtgcgtc cagcgattgt gcgcgtgaga
     6241 tacgcggcac agaatccatc gccatcacgg tcacgttacg ttccgcaagt ttttgcatta
     6301 attccggatt ctgcgcaggc cagataaaac tcaccagcgt tgtcccagga ttcagtaacg
     6361 caatttcatc atctaacggc gcattgacct tcagaatgat ctctgactgc cagacgctat
     6421 tcccttctac aatttcagcg cccgcttgca caaacgcttt atcgtcaaaa cttgccagtt
     6481 gacccgcgcc gctctctacc gcgacggtaa aacccagttt cagcagctgt tccactgttt
     6541 ttggcgttgc tgcaacacgg gtttcattgg ttaaccgttc tcttggtatg ccaattcgca
     6601 tgatattccc ttccatcggt tttattgatg atggtttgcc tgtgtcagga gccacacaag
     6661 ccgctcatgt acgagctaaa tgttactccg ttaaaataaa ttagtaacaa acgccctata
     6721 acgtactgaa aattatgcct gtgatctagc gccaaaaatc agtatttcgg cgtgaactcg
     6781 caaaatatta acgattcagc cgtgatagtg ggataaacac cttagaacgc cggataaaga
     6841 ctgataattg tcttcgacgg ccgggtaaaa cgagacatcg ccccggcacg attcactact
     6901 taacattaaa ttaacttata caattcagtt gcttcagtag taatgatgct gatacggctg
     6961 ttttttaagc atagacggtc atttgagcag gattaaaatt ggcttaagga atgtgatatg
     7021 aaaaatgacg cagacagtta caccgtttaa atgcaataat cagccacgtt tctcgttaat
     7081 aacaatacca gtacctggtt tgcgcaaggc gaaggattat ttttatgaag cttaagaaca
     7141 ccctcctggc gtcggcactg ctttctgcta cggcattctc cgttaacgca gcaacagaac
     7201 tgacaccgga gcaagcggca gcggttaaac cttttgaccg tgtagtggtt accggtcgtt
     7261 ttaatgctat tggcgaagcg gtgaaagccg tttctcgtcg cgcagataaa gaaggtgccg
     7321 cctcttttta tgttgtcgac acttctgatt ttggtaacag cggtaactgg cgtgtggtcg
     7381 ctgacctcta taaagccgat gctgaaaaag cagaagaaac aagtaatcgc gtaattaacg
     7441 gtgttgtcga actgccgaaa gatcaggctg ttctgattga accgtttgac acggtcaccg
     7501 tccagggctt ctatcgtagc cagccagaag tcaatgatgc catcaccaaa gcggcaaaag
     7561 cgaaaggtgc ctactctttc tacatcgttc gtcaaatcga tgccaaccaa ggcggcaacc
     7621 agcgtattac tgcattcatc tataaaaaag atgctaagaa acgtatcgtc cagagcccgg
     7681 atgtgatccc ggcagattcc gaagcaggac gtgcagctct ggctgccggt ggcgaagccg
     7741 cgaagaaagt tgagatccta ggtgttgcga ctaccgcatc accaagttct gaagtcggtc
     7801 gtttctttga aacccagtca tcaaaaggcg ggcgttacac cgtcatgctc ccggatggca
     7861 ctaaagtcga agaactgaac aaagcgaccg cagcgatgat ggtcccgttc gacagcatca
     7921 aattctctgg caactacggc aacatgaccg aagtctccta tcaggttgcg aaacgtgccg
     7981 cgaagaaagg tgctaagtat taccacatca cccgccagtg gcaggaacgt ggtaataacc
     8041 tgaccgtcag cgcagatctg tataaataac tattcagtta ctcctataag gcggcttgat
     8101 gccgcctttc gcatttttag tcacaaattt ccccgcgacc attgcatgca gacacatctc
     8161 ttcgtaaaat cccgcgcttc agtggcaaac tccattttta tgcgttttgc cctaatattt
     8221 attcttctta tcacgtttta atcactggat atcgatgaaa aagaaactgg gactgagcgc
     8281 actcaccgcg ctggtattaa gctcaatgct gggcgcgggt gttttcagtc tgccgcaaaa
     8341 tatggcggca gttgccagcc cggcagcact gctcatcggc tggggtatta ctggcgctgg
     8401 cattttattg ctggcctttg ccatgctgat cctcacgcgc attcgtcccg aacttgacgg
     8461 cggtatcttt acctatgccc gcgaagggtt tggcgagcta atcggctttt gttccgcatg
     8521 gggatactgg ctgtgcgcag tcatcgccaa cgtctcctat ctggtgatcg ttttttccgc
     8581 gttaagcttt tttacggaca cgccggaatt gcgcctgttc ggcgatggta atacctggca
     8641 atcgatcgtc ggtgcatcgg ctttattgtg gattgttcac tttttgatcc tgcgcggggt
     8701 gcaaaccgct gccagcatta acctggtggc gacactggca aaattgttgc cgctgggtct
     8761 gtttgttgtg ctggcgatga tgatgttcaa actggatacc ttcaagctcg acttcaccgg
     8821 acttgcactt ggcgtacccg tttgggaaca ggtgaaaaac accatgctga tcaccctgtg
     8881 ggttttcatt ggtgtggaag gtgccgtggt tgtttctgcg cgtgcgcgta ataaacgtga
     8941 tgttggcaaa gcgacactgc tggcggttct ctccgctctg ggcgtttact tgctggtaac
     9001 gctgctttcg ctgggtgtgg tggcacgtcc tgaactggct gaaattcgta acccgtcaat
     9061 ggccggtctg atggtggaaa tgatgggacc atggggcgaa atcatcatcg ctgccggttt
     9121 gatcgtttcc gtttgcggcg cgtacttaag ctggaccatc atggcggcag aagttccgtt
     9181 cctggctgcc actcataaag cattcccacg catttttgcg cgccagaacg cacaagctgc
     9241 gccatccgcc tcactgtggt tgaccaacat ctgcgtgcaa atttgtctgg tactcatctg
     9301 gctgaccggt tccgattaca acacgttgct gaccatcgcc tcagaaatga ttctggtgcc
     9361 ctatttcctg gtcggcgcgt tcttgctgaa aatcgcaacc cgtccactgc ataaagcggt
     9421 aggtgtcggt gcctgcattt atggcttatg gttattgtac gcctctggtc caatgcactt
     9481 gctgttatcc gttgttctgt atgcgccggg gctgctggtt ttcctgtatg cgcgcaaaac
     9541 gcatacacat gataatgtac tgaaccgtca ggaaatggtg ctgattggca tgctgttaat
     9601 tgcctcagtt ccggcgacct ggatgctggt gggataacgt tcatcccatc gttggatagc
     9661 aaaggagata acgatgggta aagcccagcc cttgccaata ttaattactg gcggaggtcg
     9721 tcgcatcggc ctcgccctcg cctggcattt cattaatcaa aagcaaccgg tgattgtcag
     9781 ctatcggacg cactatccag ccattgatgg actgattaag gcaggtgcgc agtgtattca
     9841 ggctgatttt tcgaccaacg acggtgtgat ggcgtttgcc gatgaagtac taaaaagccc
     9901 ccatggtctg cgtgctattt tgcataacgc cagtgcgtgg atggcggaaa aaccgggtgc
     9961 gccactggcc gacgtactgg cttgcatgat gcagatccac gttaataccc catacctgct
    10021 caaccatgcg ctggaaagat tactgcgtgg gcacggacac gccgccagcg atatcattca
    10081 ctttaccgat tatgtggtgg agcgcggtag cgacaagcat attgcgtatg ctgcaagcaa
    10141 agcggcactg gataatatga cccgctcgtt tgcccgcaag ctggcaccgg aagtgaaagt
    10201 gaactctatt gctccatcgc tgatcctgtt taatgaacat gatgatgccg aatatcgaca
    10261 acaggcgctg aataaatcac tgatgaaaac cgcgcctggc gagaaagaag tgatcgacct
    10321 ggtcgattac ttacttacca gttgctttgt caccggacgc agtttcccac ttgatggcgg
    10381 tcgtcatctg cgttaatgca gctttatcca gcatataatc aacacccatg cgcttatccc
    10441 ccaacacgaa acagaaccaa caaaagcggc gggcaagcgc atcattccgg tgaaatacca
    10501 cagcgacacc aggtaaacaa aatagggaat aatcgaccac atactaaaaa tgatggttgc
    10561 gcgtaaggct tcaatgccgc gttcgctggc aacaatataa tgcgcgataa gcgcaaaggt
    10621 cgggaaaagt ggaatcagcc cggcgatata ataatttttc gtttttgcta aaacaccaat
    10681 caacagcact accagcgcac cgagcgcggc tttgattacc agccccattt ttttacctta
    10741 acacttccat aacaagtcat cagtagaata cctgatgaaa acttgtttag aaacgattga
    10801 tagtaagtaa aaacagcgcg gtgtattgtg acgtttttat atctaccgtg aatgttatga
    10861 acactatcgt atttgtggaa gatgatgcgg aagtcggttc actgattgcc gcgtacctgg
    10921 caaaacatga tatgcaggtt accgtagagc cgcgcggcga ccaggccgaa gaaaccattt
    10981 tgcgagaaaa tccggatttg gtgttactcg acatcatgct accaggcaag gacggcatga
    11041 ccatttgtcg tgatttacgc gcaaagtggt ctggaccgat tgttcttcta acctctctcg
    11101 atagcgatat gaaccacatc ctggcactgg aaatgggtgc ctgcgactat attctcaaaa
    11161 cgacgccccc tgctgttttg ctagcgcgtt tacgtttgca tttgcgtcag aatgagcaag
    11221 ccacactgac caaaggtctt caggaaacgt ctctgactcc ctacaaagcc ctgcatttcg
    11281 gcacgttgac catcgatccc atcaaccgcg tagtcaccct ggctaacact gaaatctcgc
    11341 tctcgacagc tgatttcgaa ttattgtggg aattagctac ccatgccggg caaatcatgg
    11401 accgcgatgc attgctgaaa aatttacgcg gcgtcagtta tgacggactg gatcgtagcg
    11461 tggacgtggc tatttcgcgg ttaagaaaaa aactgctcga taacgccgca gaaccttatc
    11521 gcattaaaac tgtgcgtaac aaaggctatc tttttgcgcc tcatgcatgg gaataagcga
    11581 tgaaaaaact gtttatccag ttttacctgt tattgtttgt ctgcttcctt gtgatgtctc
    11641 tgctggttgg gctggtgtac aaatttaccg ccgaacgcgc gggcaaacag tcgctggatg
    11701 atttgatgaa cagttcactg tatctgatgc gcagcgaatt gcgtgagatc cccccacacg
    11761 actggggtaa gacgctgaaa gagatggatt taaatctctc tttcgatctg cgtgtcgagc
    11821 cactgagtaa ataccatctt gatgatattt ccatgcaccg actgcgtggc ggcgaaattg
    11881 tcgccctgga cgatcagtac acgtttttgc agcgtatccc gcgcagccac tacgtgctgg
    11941 cagttggtcc tgttccttat ctttattatc tccatcagat gcgattgctg gatatcgccc
    12001 tgatcacttt tattgctatt tccctcgcct ttccggtgtt tatctggatg cgtccgcact
    12061 ggcaggatat gttaaaactg gaagcagcgg cgcaacgatt tggcgatggg catctcaatg
    12121 aacgtatcca ctttgatgag ggttcgagct ttgaacgact tggtatcgca tttaaccaga
    12181 tggcggacaa tatcaacgcc ttgattgcca gcaaaaaaca gcttattgac ggtatcgctc
    12241 acgaactgcg aacaccgtta gtgcgcctgc gttatcgact ggagatgagc gataacctga
    12301 gcgccgccga atcccaggcg ttgaatcgtg atatcagtca acttgaagct ttaattgaag
    12361 agctgctgac ttatgcccga ctcgatcgcc cacaaaacga gcttcatctt agcgaaccag
    12421 acctgccgtt gtggctgtca acgcatctgg cagacattca ggcagtaacg cccgataaaa
    12481 cggtacggat taaaacgctc gcgcaaggcc attatgcggc gttggatatg cgcttaatgg
    12541 agcgcgtgct ggataatttg ctcaataacg ccctgcggta ctgccattca acggttgaaa
    12601 ccagcctgct actgtcgggg aatagagcga cattaattgt tgaggatgat ggcccaggga
    12661 ttgccccaga aaaccgcgaa catatctttg aaccttttgt tcgcctcgat cccagccggg
    12721 atcgctcaac cggcggctgc gggctggggc tggcaattgt ccactctata gcactggcaa
    12781 tgggcggtac ggttaattgt gacaccagcg aactgggtgg tgctcgcttc tcgtttagct
    12841 ggccgttatg gcataacatc ccgcaattta cctctgcctg acactacgcg cacgatggtc
    12901 aagtcaccac cactgtgcta taaaataagt atgttgtaac taaagtggtt aatattatgg
    12961 cgcgttacga tctcgtagac cgactcaaca ctacctttcg ccagatggaa caagagctgg
    13021 ctgcatttgc cgctcatctt gagcaacaca agctattggt tgcccgcgtg ttctctttgc
    13081 cggaggtaaa aaaagaggat gagcataatc cgcttaatcg tattgaggta aaacaacatc
    13141 tcggcaacga cgcgcagtcg ctggcgttgc gtcatttccg ccatttattt attcaacaac
    13201 agtccgaaaa tcgcagcagc aaggccgctg tccgtctgcc tggcgtgttg tgttaccagg
    13261 tcgataacct ttcgcaagca gcgttggtca gtcatattca gcacatcaat aaactcaaga
    13321 ccacgttcga gcatatcgtc acggttgaat cagaactccc caccgcggca cgttttgaat
    13381 gggtgcatcg tcatttgccg gggctgatca cccttaatgc ttaccgcacg ctcaccgttc
    13441 tgcacgaccc cgccacttta cgctttggtt gggctaataa acatatcatt aagaatttgc
    13501 atcgtgatga agtcctggca cagctggaaa aaagcctgaa atcaccacgc agtgtcgcac
    13561 cgtggacgcg cgaggagtgg caaagaaaac tggagcgaga gtatcaggat atcgctgccc
    13621 tgccacagaa cgcgaagtta aaaatcaaac gtccggtgaa ggtgcagccg attgcccgcg
    13681 tctggtacaa aggagatcaa aaacaagtcc aacacgcctg ccctacaccg ctgattgcac
    13741 tgattaatcg ggataatggc gcgggcgtgc cggacgttgg tgagttgtta aattacgatg
    13801 ccgacaatgt gcagcaccgt tataaaccac aggcgcagcc gcttcgtttg atcattccac
    13861 ggctgcacct gtatgttgcg gattaacgcc cggctttcat actgccgacc atctgttctg
    13921 gccgtaccca gctgtcaaac tcggcttcgc taagataccc cagcgcaagg gccgcagctt
    13981 ttaaggtcag cccttcttta tgcgcttttt tggcgatctc cgcggctttg tcataaccaa
    14041 tgtgggtgtt aagcgcagtc accagcatca gcgattcatt gagtaattga ttgattcgct
    14101 cacgattcgg ttcaataccc actgcgcagt gtttgttaaa actttccatg ccatctgcca
    14161 gcaagcgcac cgattgcagg aaattgtgga tcaccattgg acggaagacg ttcagttcaa
    14221 agttaccgga agcgccaccc atgttgatcg ccacgtcgtt ccccattacc tgacagcaga
    14281 gcatggttaa tgcttcacac tgggttgggt tcactttccc cggcatgatt gagctgcccg
    14341 gctcattttc cgggattgag atttcaccaa ttccgcagcg cgggccagag gccagccagc
    14401 ggacatcatt ggcgattttc atcagtgacg cagccaaccc tttcaacgcg ccgtgcgcct
    14461 gaaccagggc atcacaggtc gcaagcgctt caaatttgtt cggcgcggta acaaacggtg
    14521 cacaggtaat gactgccagt tcatctgcta cgcgacgcgc atactccgga tgggtattta
    14581 gtccagtacc caccgctgta ccgccgagag ccagttccgc tacgtgaggc aggctgtatt
    14641 cgatatgttt gagattatgc tcgagcatcg ctacccagcc ggaaatctcc tgccccagtg
    14701 ttagcggcgt ggcgtcctgc aagtgggttc gaccgatttt gacgatatcg gcaaatgcac
    14761 gcgatttttc actcagtgtc tgtgtcaggg ttttaacctg cggaatgagt tgcttgcgca
    14821 gcgccagcag cgccgcaacg tgcatcgccg tcggaaagac atcgttggaa ctttggcttt
    14881 tgttcacgtc gtcgttaggg tgaactttac gttccatccc gcgcacgccg ccgagtaatt
    14941 cactggcccg gttagccagc acttcgttca tgttcatgtt actttgcgtg ccggagccgg
    15001 tctgccagat agccagcggg aattcgtcgt catgctgtcc tgccagtact tcatccgccg
    15061 cctgccgaat ggcgctcgct ttctcttcag acaacaagcc taaatcttca ttaacttttg
    15121 ccgctgcgcg cttggttagc gccagcgcat gaatcagtga ggtgggcatt ttctccgtcg
    15181 aaatgcggaa atgctccagc gagcgttgag tttgtgcgcc ccacagctta tctgccggga
    15241 catcaatcgc ccccatcgaa tctttttcgc tgcgtactgt attcatggcc tgctcctcac
    15301 ctgattaatt ttttctttct gttttgcctt cgttaagcaa cttttagctc acttattatt
    15361 tactatttga taacaaatgt ttggtctttc gtgccatgta aaaaaaccgc cccgaagggc
    15421 ggctctgttt atttcacaca gcgagtgcac tgtgtgagtt gtatctgctg gaagaagtca
    15481 tttcctttat catccacaag gataaacgcc gggaaatctt ccacttcaat tttccagatg
    15541 gcttccattc ccagttccgg atattcaaca cattccaggc tcttaatact tccctgcgcc
    15601 aatacagcgg ccggaccacc gatactgcca aggtagaagc cgccgtgttt tttacaggca
    15661 tccgtcacct gctggctgcg gttgcctttc gccagcatga tcatacttcc gccctgcgct
    15721 tgcagttgat cgacataaga atccatccgt ccggcggtcg ttgggccaag agaaccggag
    15781 gcataacctt ccggcgtttt ggccggaccc gcgtagtaaa tcggatgatc tttaatgtac
    15841 tgcggcagcc cttcaccgtt atccatccgc tctttcagtt tggcgtgagc aatatcacga
    15901 ccgacgataa tcgtgccgtt aagcgataag cgtgtagaaa cgggatactg cgacaactgt
    15961 gcgaggatct ctttcatcgg acggttaagg tcaacgcgca ccgcttcgcc ttctcccgct
    16021 ttacgcagct cttcagggat atatttacct ggattatgtt ccagtttttc gatccagatc
    16081 ccctgacggt tgatcttcgc tttgatatta cggtcagcag agcaggaaac acccatacca
    16141 accggacagg atgcgccgtg acgtggcagg cgaatcacgc gtatgtcgtg agcgaagtat
    16201 ttaccaccaa actgcgcacc cagaccgaga ttttgcgctt cgatcagcaa ttctttttcc
    16261 agttccacat cgcggaatgc ctgaccgtgc tcattccctt ccgttggcag ttcgtcatag
    16321 tatttagcgg aagccagttt cactgtttta aggttagttt ctgcagaagt tccaccaatt
    16381 acgaacgcaa tatgatacgg aggacaggcc gccgtaccca gcgtgcgcat cttctcaacc
    16441 aggtaatttt tcagtttccc cggcgttagt aacgctttgg tttcctgata gagatacgtc
    16501 ttgtttgccg aaccaccacc tttggcgata cagaggaatt tgtactcgtc gccatcaacg
    16561 gcataaagat cgatctgcgc tggcagattg gtgccggtat tcacctcttt atacatatcc
    16621 agcggcgcgt tttgcgagta gcgcaaatta tcttcgatat aagtgttata gacaccgcgc
    16681 gccagcgccg cttcatcaca gccaccggtc catacacgct gccctttttt accaacaata
    16741 atcgcagtgc cggtatcctg acaggttggc agaacacctt tcgccgcgat gtcggagttg
    16801 cgcaggaatt gcagcgccac atatttatca ttttcgctgg cttccgggtc acgcagaatg
    16861 tcggccacct gttgttggtg agccggacgc agcataaatg acgcatcatg aaacgcctga
    16921 cgcgccaaca gagttaacgc ttcgggtgcg actttcaaaa tctcctgccc ttcaaattca
    16981 gatacgctaa cgtgttcgct ggttagcagg taatactcag tatcatcttt tttgagtgga
    17041 aaaggagcct gataatgaaa gggtttgttt gacattgttc tctcacttac tgcctggttt
    17101 ggttatgctc tgggcgggtg ttccgttgcc ctgttaaaag cgagtaacaa tatcctacac
    17161 acttttttaa caaaaactga gactagtacg actttttgcg gctccaggtt acttcccgta
    17221 ggattcttgc tttaatagtg ggattaattt ccacattaaa acagggattg atcatgcaaa
    17281 aactcattaa ctcagtgcaa aactatgcct ggggcagcaa aacggcgttg actgaacttt
    17341 atggtatgga aaatccgtcc agccagccga tggccgagct gtggatgggc gcacatccga
    17401 aaagcagttc acgagtgcag aatgccgccg gagatatcgt ttcactgcgt gatgtgattg
    17461 agagtgataa atcgactctg ctcggagagg ccgttgccaa acgctttggc gaactgcctt
    17521 tcctgttcaa agtattatgc gcagcacagc cgctctccat tcaggttcat ccaaacaaac
    17581 gcaattctga aatcggtttt gccaaagaaa atgccgcagg tatcccgatg gatgccgccg
    17641 agcgtaacta taaagatcct aaccacaagc cggagctggt ttttgcgctg acgcctttcc
    17701 ttgcgatgaa cgcgtttcgt gagttttccg agattgtctc cctactccag ccagtcgcag
    17761 gtgcacatcc ggcaattgct cactttttac aacagcctaa tgccgaacgt ttaagcgaac
    17821 tgttcgccag cctgttgaat atgcagggtg aagaaaaatc ccgcgcgctg gcgattttaa
    17881 aatcggcgct cgacagtcag cagggtgaac cgtggcaaac gattcgttta atttctgaat
    17941 tttacccgga agacagtggc ctgttctccc cgctgttact gaatgtggtg aaattgaatc
    18001 ctggcgaagc gatgttcctg ttcgctgaaa caccgcacgc ttacctgcaa ggcgtggcgc
    18061 tggaagtgat ggcgaactcc gataacgtgc tgcgtgcggg tctgacgcct aagtacattg
    18121 atattccgga actggttgcc aatgtgaaat tcgaagccaa accggctaac cagttgttga
    18181 cccagccggt gaaacaaggt gcagaactgg acttcccgat tccagtggat gattttgcct
    18241 tctcgctgca tgaccttagt gataaagaaa ccaccattag ccagcagagt gccgccattt
    18301 tgttctgcgt cgaaggcgat gcaacgttgt ggaaaggttc tcagcagtta cagcttaaac
    18361 cgggtgaatc agcgtttatt gccgccaacg aatcaccggt gactgtcaaa ggccacggcc
    18421 gtttagcgcg tgtttacaac aagctgtaag agcttactga aaaaattaac atctcttgct
    18481 aagcttatta aaggcttata acaccttcag gcggccagtc cgcctgattt cattttatgg
    18541 ataatcatta tgaataaatc gctggtagcg gtaggcgtca ttgttgcgct aggcgtagtc
    18601 tggacaggcg gcgcatggta tacaggcaag aagattgaaa cccatctcga agacatggtc
    18661 gcgcaggcga acgcgcaact caaactgaca gctcctgaat ccaacctgga agtgagttat
    18721 caaaactatc atcgcggcgt attcagcagc cagttgcaac tgttggtgaa acccattgcc
    18781 gggaaagaaa atccgtggat taaaagcggt cagagcgtca tcttcaacga atcggttgat
    18841 catggtccct tcccgcttgc ccagcttaaa aaactgaacc tgatcccgtc gatggcatca
    18901 attcaaacca cgctggttaa taacgaagta agcaaaccac tgtttgatat ggcaaaaggt
    18961 gaaacgcctt ttgagattaa ctcgcgcatt ggttacagcg gtgattccag ttccgatatt
    19021 tcgctcaagc cactgaatta cgagcaaaag gatgaaaaag tcgcctttag cggcggcgag
    19081 ttccagttaa atgctgacag agacggcaaa gccatctccc tttccgggga ggcgcaaagt
    19141 ggtcggatag acgcagttaa cgaatacaac cagaaagtgc agttgacctt taataatctg
    19201 aaaaccgacg gttccagcac gctggcaagt tttggtgagc gcgtaggaaa ccaaaaactg
    19261 tcactggaaa aaatgaccat ttcagtggaa ggcaaagaac tggcactgct ggaaggcatg
    19321 gagatcagcg gtaaatcgga tctggtcaat gacggtaaaa cgatcaatag ccaactggat
    19381 tactcgctaa acagcctgaa ggtacagaat caggatctgg gcagcggcaa gctgacttta
    19441 aaagtcggcc agattgatgg tgaagcctgg catcagttta gccagcaata taacgcgcaa
    19501 actcaggcgc tgctggcaca gccagaaatt gccaacaatc ccgaacttta tcaggagaaa
    19561 gtgacggaag ccttctttag cgccctgccg ctgatgttga aaggcgatcc ggtgattact
    19621 atcgcgccgc taagctggaa aaacagtcag ggtgaaagtg cgctgaatct gtcgctgttc
    19681 ctgaaagatc cggcaacgac taaagaagcg ccgcaaacgc tggcgcagga agtagatcgt
    19741 tcggttaaat ctctggatgc gaaactgacc attccggtgg atatggcaac tgagtttatg
    19801 actcaggtag cgaagctgga aggttatcag gaagatcaag cgaaaaaact ggcgaaacag
    19861 caagttgaag gtgcatcagc aatggggcag atgttccgtc tgaccacctt gcaggacaat
    19921 accatcacca ccagcctgca atatactaac ggtcagataa cgttaaacgg gcagaaaatg
    19981 ccactggaag atttcgttgg tatgtttgcg atgccagctc ttaacgttcc ggctgtaccg
    20041 gcaattccgc agcagtaatt cacggccaaa acgccggaga attttcactt cggcgttttc
    20101 atctgaaaac aaaccagaga ttgcctgatg cgctacgctt atcaggccta ccttttcctg
    20161 caacactttg aatttatgag tttttgtagg ctggataagg cgtttacacc gcatccggca
    20221 taaaaaacgc gcactttgtc aacaatctga aacgccggag gtttttctct ccggcgttat
    20281 ttctgacttc agcataaagt catactgaat actcattttc acatcccagt aattcaggcg
    20341 atcgcccagc gtactgccat cgcgcttttc ccgcccttta tcggcagcaa gaaagaaatt
    20401 catcccttgt aacggccctt cgctgacccg atagttcata ccacctaaga tacccgcgcc
    20461 ttcaatccgc acatctcgtt tactggattc atagccgcga gtcacaatct gtgcaaatgt
    20521 cagtgtaaat tgcggcgtta aacgatagtt gacgcccaat tgccaggact gcattccttc
    20581 acggttacga tcaagactta atgaaaaggg atagccaatg tcggtatcca ccacggcacc
    20641 attcattgca tggcgcaaat aaccaccact gccaaatacg cttccccacg gcgcattcca
    20701 ggtcaattgt ccactaacca acgccgtttc attgggctgg ctggtattac gacttaaacc
    20761 ttcgagctgc gcgtaaaagc cgagcaactc ccctttcaac gtataattcg ggtcgagcgg
    20821 tatgcttttc tcgagaatac tttgtatctg attggaaaca tctttctgta ccgatgcccc
    20881 cagagaatag cgactggtat catcatgcca actggcggct aaaaaagaac gtgcgcgatg
    20941 tttatcgtat tttcccgtct ggctgttata caccccttca tcacgaatac cggttgttga
    21001 atcagtccat aaaaatactc gtgaacgcca cattccccac agatcaatat ttggtgttaa
    21061 agcccaataa cctaatgctc cttcagaaac tgtcggtaat gtacgttgcg aactgccagc
    21121 cattaacgga atacgataag gtaagctacc ataactataa tccgtcccga agcgacctga
    21181 tttaagcagg ctattttcac tgggttttat ttttactgcc cccagggcaa agccaaaact
    21241 tttatcacca tcaagatacc accgggtact catatcagca cgagcaccta atttataaac
    21301 ataatatgcc ccgccttcaa cgccaataat attggaataa taaccactat tgaaatcgag
    21361 caatcctccc tgtacccatg cgtaaatatc tcttcccgcg ccagcactgg gcttatccgc
    21421 cctgcgcaat tcgtttttca gtctgatgcg tagtcccgcc tcatcgtcag ccagttgcgc
    21481 cgcataagca gagataaggc cagaggcagc cgtcaggcaa ataacggcca tggccactat
    21541 ttttctcata gttgcacctt tgatgttctg attattttta ttgattatta attagtgata
    21601 tcgctgatta attgctgctg cactttttta cgattatcaa tttcaaccac gatttctttg
    21661 aattttttat ccgtaagcgg ataaaaccag ataataacga atgccagtag cataaatccg
    21721 caaggtacta aggcaattga tgtgcggatg cccataataa cttccggcgt ttgcacctga
    21781 ttggcaatat atccgcttaa ccccaaaata aaggcaggaa ttgaacctcc gattgcctga
    21841 ccgcatttac gggtaaatga gaatagtgaa taggtgagcc cttcaattcg cacgccggtc
    21901 aggtattcac cgtattctac ggtatcagct tccagtgccc acatcactgt catagtaacg
    21961 ccctgaccaa ttgaagcgat ggccaacgca accaacgcca ccggcagtga ccagacggaa
    22021 acccagaaga atagcagata accgcaggtt cccagcaaag cgccaatcag gaaggtattc
    22081 tttttaccga tcctcgcgac catccccggc acaagcggtg ccgatgccac agtaccaacc
    22141 aggttttgca ccagtaccag cacagtgaac agcccggtat catttaacac atagcgcacg
    22201 tagaacaacg acgaggcgct gaccgcaaag gtcgaaatca gcacacacag cgcaccgatg
    22261 cacaacataa acagcgggcg attccgtttc agggtttgca aactgatctt caatgacggc
    22321 tgcgcaacga tacgtaccac attctcacgc gtcgatttga agcagatgaa gtaaagcacc
    22381 attccggcaa tcgccagcac aatcgtccag aaatggtata ccgacaccat ctcttccggg
    22441 ctggagttct taatgctcgg ccctatcaga aatgccaggc agacaaaggt caatgaagcg
    22501 gcaatcccac gagccgcgcc cagacgggcg cgggattgtg gttgttgggt catcgcggta
    22561 gcaagtgaac cataaggaat attcaccagg ctgtagcaaa gcccgaggcc catgtaggtc
    22621 aaatatgcat acactacttt gcggctatgg ctccagtcgg tcggtaccca gaataccagc
    22681 acgctgaaga tcattaacgg cgcagtaccg aagagtaaaa acgggcggaa ttttccccag
    22741 cgggtattca cactgtccac cactcgtccg gcaaagacgt cggcgaaggc atcgaatacc
    22801 cgcaccagta acagcatggt gcccgccgca gcggcaccga cgccagcgac gtcggtgtag
    22861 taactcaaca ggaagagcac ccccattgcg aaggcgaagt tattggcgac gtcaccgagg
    22921 ctgtagccga cgatggtgcg ccaggagagt tgttgattca ttgtttgcct ccctgctgcg
    22981 gtttttcacc gaagttcatg ccagtccagc gtttttgcag cagaaaagcc gccgacttcg
    23041 gtttgcggtc gcgggtgaag atccccttct tgttaccgcc aacgcgcaat atgccttgcg
    23101 aggtcgcaaa atcggcgaaa ttccatacct gttcaccgac gacggcgctg acgcgatcaa
    23161 agacgcggtg atacatatcc agccatgcac actgatactc ttcactccac atgtcggtgt
    23221 acattgagtg cagcccggct aacgtatcca cgccgtattc ggtgatgata atcggctgat
    23281 gcagtttctc ctgccaggcc agaagttctt tttccagtac cttctctgcc gtttccaaat
    23341 cgccgctttg gacataccat ccgtaataac ggttcaggca cagcacatca aagagatcgc
    23401 tgatggtatc ggtgtgagcg tcgcagaaca ttacattgac gcaggtgatc ggacgcgtcg
    23461 ggtcgagttt acgcgttgct tccgccagtg gcgcgaaata ttcccgtgca ccttgcggac
    23521 gggtatccgg ttcgttggca atactccaca tcaccacgct tgggtggttt ttgtcacgcg
    23581 ctatcagctc tttaatcgcc tgtaagtgcg cttgctgagt ttccccgttg actgcctctt
    23641 cgctgtacag ttctttcggc ttgttgcccg cttcgaaacc aatgcctaaa gagaggttaa
    23701 agccgacagc agcagtttca tcaatcacca cgatgccatg ttcatctgcc cagtcgagca
    23761 tctcttcagc gtaagggtaa tgcgaggtac ggtaggagtt ggccccaatc cagtccatta
    23821 atgcgtggtc gtgcaccatc agcacgttat cgaatccttt gccacgcaag tccgcatctt
    23881 catgacgacc aaagccagta aagtagaacg gtttgtggtt aatcaggaac tgttcgccct
    23941 tcactgccac tgaccggatg ccgacgcgaa gcgggtagat atcacactct gtctggcttt
    24001 tggctgtgac gcacagttca tagagataac cttcacccgg ttgccagagg tgcggattca
    24061 ccacttgcaa agtcccgcta gtgccttgtc cagttgcaac cacctgttga tccgcatcac
    24121 gcagttcaac gctgacatca ccattggcca ccacctgcca gtcaacagac gcgtggttac
    24181 agtcttgcgc gacatgcgtc accacggtga tatcgtccac ccaggtgttc ggcgtggtgt
    24241 agagcattac gctgcgatgg atcccggcat agttaaagaa atcatggaag taagactgct
    24301 ttttcttgcc gttttcgtcg gtaatcacca ttcccggcgg gatagtctgc cagttcagtt
    24361 cgttgttcac acaaacggtg atacgtacac ttttcccggc aataacatac ggcgtgacat
    24421 cggcttcaaa tggcgtatag ccgccctgat gctccatcac ttcctgatta ttgacccaca
    24481 ctttgccgta atgagtgacc gcatcgaaac gcagcacgat acgctggcct gcccaacctt
    24541 tcggtataaa gacttcgcgc tgataccaga cgttgcccgc ataattacga atatctgcat
    24601 cggcgaactg atcgttaaaa ctgcctggca cagcaattgc ccggctttct tgtaacgcgc
    24661 tttcccacca acgctgatca attccacagt tttcgcgatc cagactgaat gcccacaggc
    24721 cgtcgagttt tttgatttca cgggttgggg tttctacagg acgtaacata agggactcct
    24781 cattaagata aaaatactgg tcaaccttta atctgattag ttaaccaatt ctgtgttgca
    24841 cacagttatc tgtgagaaac ccatggaaga gaaatggaaa taaaaagaac tgacaccgcg
    24901 atcaaaaaac caaaaactgc aatacccaca gcgtgatatt gatcgcatta atctttaaaa
    24961 cggcgttgac agaagccata aaataagcaa ccgaacgttc gttcgagcat attgggaacg
    25021 ataaactctc atgacatatg gctacggtga atatttttgg gggggaattc tccgatcccg
    25081 gctagattgt attttgtaca gggaaattaa agtatgaaac gcgggcacag ttatcaccag
    25141 cccgcgaaat gagaggatca ggatgcggtt aagataccgc caatcatcgc gcgtaatccc
    25201 tgagcaaacg acgcttcacg cgccagatct tccgcttcga tatcggccag tacaccgtag
    25261 gtcagatcca gcagtaaacg tgaagtcatt gacgtgttga tgtctgggct tatctcgcct
    25321 ttttgctggg catcacgcat ccgctgggca acaaattccg tgatcgtcat atgcttattt
    25381 ttaagcatgg cggcgacctg tgggttacgc atcccttccg ccataatttc gaccaccagc
    25441 gcccgttgcc caaaggcttc atgggtgaga gagacaatag actcgaccat atagtcaaca
    25501 aaatgaatcc ctttaatcgg ttcccggaaa cgagccagcg ccctctcctg gtcctgtaag
    25561 ataatcgcct gaatcaaggc ttctttggag atgaaatggt gatagagcgt cccgggacta
    25621 atggcgcaag atttacagat ggctttcatc gaggcactgt gaaatccatt ttctgaaaaa
    25681 atctctctgg cagcattgag gatccgggtc cgtgtcggtt gtgcttcagt ctgcatgtta
    25741 tccatcatta aaccattata gttagcgtga ataactagtt taccgcgttg tcaggtgagc
    25801 gcctatcatt gctgttgaat agtgcgaagg cacactctat tcgtatactt ttcactctga
    25861 aattggttaa ccaatagccg tgatttggct gccagttatt tagcagagac gttgatgagt
    25921 ttatcccggt aaacaacacg cacggttgtt taccggtccg ttagtgtatt aattgagctc
    25981 ctgtacccca ccaccggaga cggtgagaat ttgtccgctt acccagctcg cagcaggcga
    26041 gcaaaggaac agcgctgcgt tagcaatatc ttgcggttgg cccagacgtc tgatcggcgt
    26101 gtgctgtaac attttttgtt caatttctgg tgtaataacg gatttcaggg catcggttaa
    26161 tattgccccc ggcgcaatgc catttacccg aatatttttt tcacccaggt caaacgccat
    26221 atttctgacc agatgactgg ctgcagcttt agatgatgca taggaagtca tgtttatatt
    26281 tttattttct gccgccatag aagtgatggt cagaataacg ccaccgccat ttttttccat
    26341 ttctggcgca acaagttgtg acagatggaa aaaagaaaac acattcagtt cataagcacg
    26401 gcgaaaatcc gccattggca tatcaaacgg tttaggtcca ccgccaccgg cgttgttaac
    26461 cagaatatca actttaccca gcttactgat agcaaagtct gccagtgcag agagttcctg
    26521 ttcggaagta atatcacaac ggcaggcaaa tgcctgacca cccagttgtt gaatttcgtc
    26581 tacaacatgg ttagctgcgt cggcgttaat atcactgacc accacagatg cgccagctgt
    26641 cgcgaatgta atggcgattt ctttaccaat acctgcaccc gcacctgtga tgatggcgca
    26701 tggcgcatgg cgcatggcgc atggcgcatg gcgcatggcg catggcgcat ggcgcatggc
    26761 gcattttccg tc
//
LOCUS       DAJTZL010000040        25494 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.040, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000040 DAJTZL010000000
VERSION     DAJTZL010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25494
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.040"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            15..485
                     /locus_tag="OO544_001485"
     CDS             15..485
                     /locus_tag="OO544_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HCR6767111.1"
                     /translation="MVDQNLPWSASLKVGDKKLPVVVVRVGEASVDVLVGQQTWTLTH
                     KWFESVWTGDYLLLWKMSPEGESTITRDSSEEEILWLETMLNRALHISTEPSAEWRPL
                     LVEKIKQFQKSHHLKTDGVVGFSTLVHLWQVAGESAYLYRDEANISPETTVKGK"
     gene            487..906
                     /gene="gspB"
                     /locus_tag="OO544_001486"
     CDS             487..906
                     /gene="gspB"
                     /locus_tag="OO544_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative general secretion pathway protein GspB"
                     /protein_id="HCR6767112.1"
                     /translation="MFEFYIAAREQKETGHPGIFSRQKHSTIIYVICLLLICLWFAGM
                     VLVGGYARQLWALWIVKAEVTVEAETPAFKQSTQHYFFKKQPLPVVESVEEEDDPDVA
                     VENAPSSSEDEENTVKESDEKAGLRERVKNALNELER"
     gene            1144..1455
                     /gene="rpsJ"
                     /locus_tag="OO544_001487"
     CDS             1144..1455
                     /gene="rpsJ"
                     /locus_tag="OO544_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HCR6767113.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            1488..2117
                     /gene="rplC"
                     /locus_tag="OO544_001488"
     CDS             1488..2117
                     /gene="rplC"
                     /locus_tag="OO544_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HCR6767114.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     EVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            2128..2733
                     /gene="rplD"
                     /locus_tag="OO544_001489"
     CDS             2128..2733
                     /gene="rplD"
                     /locus_tag="OO544_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HCR6767115.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            2730..3032
                     /gene="rplW"
                     /locus_tag="OO544_001490"
     CDS             2730..3032
                     /gene="rplW"
                     /locus_tag="OO544_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HCR6767116.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3050..3871
                     /gene="rplB"
                     /locus_tag="OO544_001491"
     CDS             3050..3871
                     /gene="rplB"
                     /locus_tag="OO544_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HCR6767117.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            3888..4166
                     /gene="rpsS"
                     /locus_tag="OO544_001492"
     CDS             3888..4166
                     /gene="rpsS"
                     /locus_tag="OO544_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HCR6767118.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            4181..4513
                     /gene="rplV"
                     /locus_tag="OO544_001493"
     CDS             4181..4513
                     /gene="rplV"
                     /locus_tag="OO544_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HCR6767119.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            4531..5232
                     /gene="rpsC"
                     /locus_tag="OO544_001494"
     CDS             4531..5232
                     /gene="rpsC"
                     /locus_tag="OO544_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HCR6767120.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            5245..5655
                     /gene="rplP"
                     /locus_tag="OO544_001495"
     CDS             5245..5655
                     /gene="rplP"
                     /locus_tag="OO544_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HCR6767121.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            5655..5846
                     /gene="rpmC"
                     /locus_tag="OO544_001496"
     CDS             5655..5846
                     /gene="rpmC"
                     /locus_tag="OO544_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HCR6767122.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            5846..6100
                     /gene="rpsQ"
                     /locus_tag="OO544_001497"
     CDS             5846..6100
                     /gene="rpsQ"
                     /locus_tag="OO544_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HCR6767123.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            6265..6636
                     /gene="rplN"
                     /locus_tag="OO544_001498"
     CDS             6265..6636
                     /gene="rplN"
                     /locus_tag="OO544_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HCR6767124.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            6647..6961
                     /gene="rplX"
                     /locus_tag="OO544_001499"
     CDS             6647..6961
                     /gene="rplX"
                     /locus_tag="OO544_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HCR6767125.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            6976..7515
                     /gene="rplE"
                     /locus_tag="OO544_001500"
     CDS             6976..7515
                     /gene="rplE"
                     /locus_tag="OO544_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HCR6767126.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            7530..7835
                     /gene="rpsN"
                     /locus_tag="OO544_001501"
     CDS             7530..7835
                     /gene="rpsN"
                     /locus_tag="OO544_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HCR6767127.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            7869..8261
                     /gene="rpsH"
                     /locus_tag="OO544_001502"
     CDS             7869..8261
                     /gene="rpsH"
                     /locus_tag="OO544_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HCR6767128.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            8274..8807
                     /gene="rplF"
                     /locus_tag="OO544_001503"
     CDS             8274..8807
                     /gene="rplF"
                     /locus_tag="OO544_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HCR6767129.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGVDKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            8817..9170
                     /gene="rplR"
                     /locus_tag="OO544_001504"
     CDS             8817..9170
                     /gene="rplR"
                     /locus_tag="OO544_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HCR6767130.1"
                     /translation="MDKKFARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            9185..9688
                     /gene="rpsE"
                     /locus_tag="OO544_001505"
     CDS             9185..9688
                     /gene="rpsE"
                     /locus_tag="OO544_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HCR6767131.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            9692..9871
                     /gene="rpmD"
                     /locus_tag="OO544_001506"
     CDS             9692..9871
                     /gene="rpmD"
                     /locus_tag="OO544_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HCR6767132.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            9875..10309
                     /gene="rplO"
                     /locus_tag="OO544_001507"
     CDS             9875..10309
                     /gene="rplO"
                     /locus_tag="OO544_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HCR6767133.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            10317..11648
                     /gene="secY"
                     /locus_tag="OO544_001508"
     CDS             10317..11648
                     /gene="secY"
                     /locus_tag="OO544_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HCR6767134.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFVGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            11680..11796
                     /gene="rpmJ"
                     /locus_tag="OO544_001509"
     CDS             11680..11796
                     /gene="rpmJ"
                     /locus_tag="OO544_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HCR6767135.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            11943..12299
                     /gene="rpsM"
                     /locus_tag="OO544_001510"
     CDS             11943..12299
                     /gene="rpsM"
                     /locus_tag="OO544_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HCR6767136.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            12316..12705
                     /gene="rpsK"
                     /locus_tag="OO544_001511"
     CDS             12316..12705
                     /gene="rpsK"
                     /locus_tag="OO544_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HCR6767137.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            12739..13359
                     /gene="rpsD"
                     /locus_tag="OO544_001512"
     CDS             12739..13359
                     /gene="rpsD"
                     /locus_tag="OO544_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HCR6767138.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            13385..14374
                     /gene="rpoA"
                     /locus_tag="OO544_001513"
     CDS             13385..14374
                     /gene="rpoA"
                     /locus_tag="OO544_001513"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HCR6767139.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            14415..14798
                     /gene="rplQ"
                     /locus_tag="OO544_001514"
     CDS             14415..14798
                     /gene="rplQ"
                     /locus_tag="OO544_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HCR6767140.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            14905..15273
                     /locus_tag="OO544_001515"
     CDS             14905..15273
                     /locus_tag="OO544_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HCR6767141.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILKGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLDKINDN"
     gene            15284..15709
                     /gene="zntR"
                     /locus_tag="OO544_001516"
     CDS             15284..15709
                     /gene="zntR"
                     /locus_tag="OO544_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HCR6767142.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            15765..15983
                     /gene="arfA"
                     /locus_tag="OO544_001517"
     CDS             15765..15983
                     /gene="arfA"
                     /locus_tag="OO544_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HCR6767143.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGAC"
     gene            complement(15980..16390)
                     /gene="mscL"
                     /locus_tag="OO544_001518"
     CDS             complement(15980..16390)
                     /gene="mscL"
                     /locus_tag="OO544_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HCR6767144.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPAPTKEEVLLAEIRDLLKEQNNRS"
     gene            complement(16520..17896)
                     /gene="trkA"
                     /locus_tag="OO544_001519"
     CDS             complement(16520..17896)
                     /gene="trkA"
                     /locus_tag="OO544_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HCR6767145.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(17918..19207)
                     /gene="rsmB"
                     /locus_tag="OO544_001520"
     CDS             complement(17918..19207)
                     /gene="rsmB"
                     /locus_tag="OO544_001520"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HCR6767146.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRQQEELLAEFNTSDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRVNRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVHDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGAEEGDGFFYAKLIKK"
     gene            complement(19253..20200)
                     /gene="fmt"
                     /locus_tag="OO544_001521"
     CDS             complement(19253..20200)
                     /gene="fmt"
                     /locus_tag="OO544_001521"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HCR6767147.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEEKGLPVFQPVSLRPQESQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLA"
     gene            complement(20215..20724)
                     /gene="def"
                     /locus_tag="OO544_001522"
     CDS             complement(20215..20724)
                     /gene="def"
                     /locus_tag="OO544_001522"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HCR6767148.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            20854..21978
                     /gene="dprA"
                     /locus_tag="OO544_001523"
     CDS             20854..21978
                     /gene="dprA"
                     /locus_tag="OO544_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HCR6767149.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSQIDAVGLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLLVTTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLLEHGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQRREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            21950..22423
                     /locus_tag="OO544_001524"
     CDS             21950..22423
                     /locus_tag="OO544_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HCR6767150.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFEREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            22452..22994
                     /locus_tag="OO544_001525"
     CDS             22452..22994
                     /locus_tag="OO544_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HCR6767151.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RLLKSSADGHIVKVLEGQVCPACGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            22999..23571
                     /gene="tsaC"
                     /locus_tag="OO544_001526"
     CDS             22999..23571
                     /gene="tsaC"
                     /locus_tag="OO544_001526"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HCR6767152.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDAQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            23576..24394
                     /gene="aroE"
                     /locus_tag="OO544_001527"
     CDS             23576..24394
                     /gene="aroE"
                     /locus_tag="OO544_001527"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HCR6767153.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKLLQQELSA"
     gene            24391..24648
                     /locus_tag="OO544_001528"
     CDS             24391..24648
                     /locus_tag="OO544_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HCR6767154.1"
                     /translation="MNQAIQFPDREEWDENKRCVCFPALVNGMQLTCAISGESLAYRF
                     TGGTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(24624..25178)
                     /locus_tag="OO544_001529"
     CDS             complement(24624..25178)
                     /locus_tag="OO544_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HCR6767155.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         6492 a         6022 c         6679 g         6301 t
ORIGIN      
        1 cttctttaca aacattggta gatcagaatt tgccctggag cgcatcgctt aaagtcgggg
       61 acaaaaaatt gcctgttgtg gtcgttcgcg tcggtgaggc tagcgttgat gtcctcgtag
      121 gtcagcaaac gtggacgctg acacataaat ggtttgagtc ggtatggacg ggtgattatc
      181 ttctgttgtg gaaaatgtcg ccggaagggg agagtaccat tacgcgtgat agcagcgagg
      241 aagagattct ctggctggaa acgatgttaa accgtgcatt gcatatttcg acagaacctt
      301 cggcggagtg gcgtcccttg ttggtagaaa aaataaagca atttcaaaaa agccaccact
      361 tgaaaactga cggtgttgtt ggcttttcaa cgttagtaca tctctggcag gtggcagggg
      421 agagtgccta cttatatcgg gatgaagcaa atatttcccc ggaaacaaca gtgaagggga
      481 aatgacatgt ttgaattcta tatcgccgcg cgtgagcaga aagaaacagg tcaccctggt
      541 atcttctccc gacaaaaaca cagcaccata atatatgtca tttgtttatt gctgatttgc
      601 ctgtggtttg caggaatggt gttggttggt gggtatgcca ggcagctatg ggcgctttgg
      661 atagtaaaag cagaagtcac tgtagaggct gaaacgcccg cttttaaaca atcaacacaa
      721 cactatttct tcaaaaaaca gccactcccc gtcgtcgaat ccgtcgagga ggaagatgac
      781 ccagacgtcg cggttgagaa tgctccctct tcttctgaag acgaagaaaa tactgtaaaa
      841 gaaagcgacg agaaagccgg tttaagagag cgtgtcaaaa atgcgctgaa cgaacttgag
      901 agataacccg aaggctgttt actttactag caatacgctt gcgttcggtg gttaagtatg
      961 tataatgcgc gggcttgtcg tagttgacag caggttcaat ctgaacctca gttagccgaa
     1021 tttggctacc taacaatgct cccaatcggg gagctacgta agaacggtta cactctccca
     1081 tcaatcgtaa tgggtctgag gagtaatcat tttcgtttat aaaataattg gagctctggt
     1141 ctcatgcaga accaaagaat ccgtatccgc ctgaaagcgt ttgatcatcg tctgatcgat
     1201 caagcaaccg cggaaatcgt cgagactgcc aagcgcactg gtgcgcaggt ccgtggtccg
     1261 atcccgctgc cgacacgcaa agagcgcttc actgttctga tctccccgca cgtcaacaaa
     1321 gacgcgcgcg atcagtacga aatccgtact cacttgcgtc tggttgacat cgttgagcca
     1381 accgagaaaa ccgttgatgc tctgatgcgt ctggatctgg ctgccggtgt agacgtgcag
     1441 atcagcctgg gttaatcagg tcattgagcg attgagaggt tgaaacaatg attggtttag
     1501 tcggtaaaaa agtgggtatg acccgtatct tcacagaaga cggcgtttct atcccagtaa
     1561 ccgtaatcga agttgaagca aaccgcgtta ctcaggttaa agacctggct aacgatggct
     1621 accgtgctat tcaggtgacc accggtgcta aaaaagctaa ccgtgtgacc aagcctgaag
     1681 ctggccactt cgctaaagct ggcgtagaag ctggccgtgg tctgtgggaa ttccgcctgg
     1741 ctgaaggcga agagttcact gtaggtcaga gcattagcgt tgaactgttt gctgaggtta
     1801 aaaaagttga cgtaactggc acctctaaag gtaaaggttt cgcaggtacc gttaagcgct
     1861 ggaacttccg tacccaggac gctactcacg gtaactcctt gtctcaccgc gttccgggtt
     1921 ctatcggtca gaaccagact ccgggcaaag tgttcaaagg caagaaaatg gcaggtcaga
     1981 tgggtaacga acgtgtaacc gttcagagcc ttgacgtagt acgcgttgac gctgagcgca
     2041 acctgctgct ggttaaaggt gctgtcccgg gtgcaaccgg tagcgacctg atcgttaaac
     2101 cagctgtgaa ggcgtaagga gatagcaatg gaattagtat tgaaagacgc gcagagcgcg
     2161 ctgactgttt ccgaaactac cttcggtcgt gatttcaacg aagcgctggt tcaccaggtt
     2221 gttgttgctt atgcagctgg tgctcgtcag ggtactcgtg ctcagaagac tcgtgctgaa
     2281 gtaactggtt ccggtaaaaa accgtggcgc cagaaaggca ccggccgtgc gcgttctggt
     2341 tctatcaaga gcccgatctg gcgttctggt ggcgtgacct ttgctgctcg tccgcaggac
     2401 cacagtcaaa aagttaacaa gaagatgtac cgcggcgcgc tgaaaagcat cctgtccgaa
     2461 ctggtacgtc aggatcgtct gatcgttgtc gagaagttct ctgtagaagc gccgaaaact
     2521 aagctgctgg cacagaaact gaaagacatg gctctggaag atgtgctgat catcaccggt
     2581 gagctggacg aaaacctgtt cctggctgcg cgcaacctgc acaaggttga cgtacgcgat
     2641 gcaactggta tcgacccggt tagcctgatc gccttcgaca aagtcgtaat gactgctgat
     2701 gctgttaagc aagttgagga gatgctggca tgattcgtga agaacgtctg ctgaaggtgc
     2761 tgcgtgcacc gcacgtttct gaaaaagcgt ctactgcgat ggaaaaatcc aacaccatcg
     2821 tactcaaagt tgctaaagac gcgaccaaag cagaaatcaa agctgctgtg cagaaactgt
     2881 ttgaagtcga agtcgaagtc gttaacaccc tggtagttaa agggaaagtt aaacgtcacg
     2941 gacagcgtat cggtcgtcgt agcgactgga aaaaagctta cgtcaccctg aaagaaggcc
     3001 agaatctgga cttcgttggc ggcgctgagt aagtcggagg agtaatacaa tggcagttgt
     3061 taaatgtaaa ccgacatctc cgggtcgtcg ccacgtagtt aaagtggtta accctgagct
     3121 gcacaagggc aaaccttttg ctccgttgct ggaaaaaaac agcaaatccg gtggtcgtaa
     3181 caacaatggc cgtatcacca ctcgtcatat cggtggtggc cacaagcagg cttaccgtat
     3241 tgttgacttc aaacgcaaca aagacggtat cccggcagtt gttgaacgtc ttgagtacga
     3301 tccgaaccgt tccgcgaaca tcgcgctggt tctgtacaaa gacggtgaac gccgttacat
     3361 cctggcccct aaaggcctga aagctggcga ccagattcag tctggcgttg atgctgcaat
     3421 caaaccaggt aacaccctgc cgatgcgcaa catcccggtt ggttctactg ttcataacgt
     3481 agaaatgaaa ccaggtaaag gcggtcagct ggcacgttcc gctggtactt acgttcagat
     3541 cgttgctcgt gatggtgctt atgtcaccct gcgtctgcgt tctggtgaaa tgcgtaaagt
     3601 agaagcagac tgccgtgcaa ctctgggcga agttggcaat gctgagcata tgctgcgcgt
     3661 tctgggtaaa gcaggtgctg cacgctggcg tggtgttcgt ccgaccgttc gcggtaccgc
     3721 gatgaacccg gtagaccacc cacatggtgg tggtgaaggt cgtaactttg gtaagcaccc
     3781 ggtaactccg tggggcgttc agaccaaagg taagaagacc cgcagcaaca agcgtactga
     3841 taaattcatc gtacgtcgcc gtagcaaata attttagagg ataagccatg ccacgttctc
     3901 tcaagaaagg tccttttatt gacctgcact tgctgaagaa ggtagagaaa gcggtggaaa
     3961 gcggagacaa gaagcccctg cgcacttggt cccgtcgttc aacgatcttt cctaacatga
     4021 tcggtttgac catcgctgtc cataatggtc gtcagcacgt tccggtattt gtgaccgacg
     4081 aaatggtcgg tcacaaactg ggtgaattcg caccgactcg tacttatcgc ggccacgctg
     4141 ctgataaaaa agcgaagaag aaataaggta ggaggaagag atggaaacta tcgctaaaca
     4201 tcgccatgct cgttcttctg ctcagaaggt tcgccttgtt gctgacctga ttcgcggtaa
     4261 gaaagtgtcg caggctctgg atattttgac ctacaccaac aagaaagcgg ctgtactggt
     4321 caagaaagtt ctggaatctg ccattgctaa cgctgaacac aacgatggcg ctgacattga
     4381 cgatctgaaa gttacgaaaa ttttcgtaga cgaaggcccg agcatgaagc gcattatgcc
     4441 gcgtgcaaaa ggtcgtgcag atcgcatcct gaagcgcacc agccacatca ctgtggttgt
     4501 gtccgatcgc tgagactctg gagactagca atgggtcaga aagtacatcc taatggtatt
     4561 cgcttgggta ttgtaaaacc atggaactct acctggtttg cgaacaccaa agaattcgct
     4621 gacaacctgg acagcgattt taaagtacgt cagtacctga ctaaggaact ggctaaagcg
     4681 tctgtatctc gtatcgttat cgagcgtccg gctaagagca tccgtgtaac cattcacact
     4741 gctcgcccgg gtatcgttat cggtaaaaaa ggtgaagacg tagaaaaact gcgtaaggtc
     4801 gtagcggaca tcgctggcgt tcctgcacag atcaacatcg ccgaagttcg taagcctgaa
     4861 ctggacgcaa aactggttgc tgacagcatc acttctcagc tggaacgtcg cgttatgttc
     4921 cgtcgtgcta tgaagcgtgc tgtacagaac gcaatgcgtc tgggcgctaa aggtattaaa
     4981 gttgaagtta gcggccgtct gggcggcgcg gaaatcgcac gtaccgaatg gtaccgcgaa
     5041 ggtcgcgtac cgctgcacac tctgcgtgct gacatcgact acaacacctc tgaagcgcac
     5101 accacttacg gtgtaatcgg cgttaaagtg tggatcttca aaggcgagat cctgggtggt
     5161 atggctgctg ttgaacaacc ggaaaaaccg gctgctcagc ctaaaaagca gcagcgtaaa
     5221 ggccgtaaat aaggagcgtc gctgatgtta caaccaaagc gtacaaaatt ccgtaaaatg
     5281 cacaaaggcc gtaaccgcgg tctggcgcag ggtacggatg ttagcttcgg cagcttcggt
     5341 ctgaaagctg ttggccgtgg tcgtctgact gcccgtcaga tcgaagcagc acgtcgtgct
     5401 atgacccgtg cagttaagcg tcaaggtaag atctggatcc gtgtgttccc ggacaaaccg
     5461 atcactgaaa agccgctggc agtgcgtatg ggtaaaggta aaggtaacgt ggagtattgg
     5521 gttgccttga ttcagccggg taaagtcctg tatgaaatgg acggtgttcc ggaagagctg
     5581 gcccgtgaag cattcaagct ggcagcagcg aaactgccga ttaaaaccac ctttgtaact
     5641 aagacggtga tgtaatgaaa gcaaaagagc tgcgtgagaa gagcgttgaa gagctgaaca
     5701 ccgagctgct gaacctgctg cgtgagcagt tcaacctgcg tatgcaggct gcaagtggcc
     5761 agctgcaaca gtctcacctg ttgaagcaag tgcgtcgcga tgtcgcacgc gttaagactt
     5821 tactgaacga gaaggcgggt gcgtaatgac cgataaaatc cgtactctgc aaggtcgcgt
     5881 tgttagcgac aaaatggaga aatccattgt tgttgctatc gaacgttttg tgaaacaccc
     5941 gatctacggt aaattcatca agcgtacgac caaactgcac gtacatgacg agaacaacga
     6001 atgcggtatc ggtgacgtgg ttgaaatccg cgaatgccgt ccgctgtcca agactaaatc
     6061 ctggacgctg gttcgcgttg tagagaaagc ggttctgtaa tacagtacac tctctcgata
     6121 cgaataaacg gctcagaaat gagccgttta ttttttctac ccatatcctt gaagcggtgt
     6181 tataatgccg cgccctcgat atggggattt ttaacgacct gattttcggg tctcagtagt
     6241 agttgacatt agcggagcac taaaatgatc caagaacaga ctatgctgaa cgtcgccgac
     6301 aactccggtg cacgtcgcgt aatgtgtatc aaggttctgg gtggctcgca ccgtcgctac
     6361 gcaggcgtag gcgacatcat caagatcacc atcaaagaag caattccgcg tggtaaggtc
     6421 aaaaaaggtg atgtgctgaa ggcggtagtg gtgcgcacca agaagggtgt tcgtcgcccg
     6481 gacggttctg tcattcgctt cgatggtaat gcttgtgttc ttctgaacaa caacagcgag
     6541 cagcctatcg gtacgcgtat ttttgggccg gtaactcgtg agcttcgtag tgagaagttc
     6601 atgaaaatta tctctctggc accagaagta ctctaaggag cgaatcatgg cagcgaaaat
     6661 ccgtcgtgat gacgaagtta tcgtgttaac cggtaaagat aaaggtaaac gcggtaaagt
     6721 taagaatgtc ctgtcttccg gcaaggtcat tgttgaaggt atcaacctgg ttaagaaaca
     6781 tcagaagccg gttccggccc tgaaccaacc gggtggcatc gttgaaaaag aagccgctat
     6841 tcaggtttcc aacgtagcaa tcttcaatgc ggcaaccggc aaggctgacc gtgtaggctt
     6901 tagattcgaa gacggcaaaa aagtccgttt cttcaagtct aacagcgaaa ctatcaagta
     6961 atttggagta gtacgatggc gaaactgcat gattactaca aagacgaagt agttaaaaaa
     7021 ctcatgactg agtttaacta caattctgtc atgcaagtcc ctcgggtcga gaagatcacc
     7081 ctgaacatgg gtgttggtga agcgatcgct gacaaaaaac tgctggataa cgcagcagca
     7141 gacctggcag caatctccgg tcaaaaaccg ctgatcacca aagcacgcaa atctgttgca
     7201 ggcttcaaaa tccgtcaggg ctatccgatc ggctgtaaag taactctgcg tggcgaacgc
     7261 atgtgggagt tctttgagcg cctgatcact attgctgtac ctcgtattcg tgacttccgt
     7321 ggcctgtccg ctaagtcttt cgacggtcgt ggtaactaca gcatgggtgt ccgtgagcag
     7381 atcatcttcc cagaaatcga ctacgataaa gtcgaccgcg ttcgtggttt ggatattacc
     7441 attaccacta ctgcgaaatc tgacgaagaa ggccgcgctc tgctggctgc ctttgacttc
     7501 ccgttccgca agtaaggtag ggttactaaa tggctaagca atcaatgaaa gcacgcgaag
     7561 taaaacgcgt agctttagct gataaatact tcgcgaaacg cgctgaactg aaagcgatca
     7621 tctctgatgt gaacgcttcc gacgaagatc gttggaacgc tgttctcaag ctgcagactc
     7681 tgccgcgtga ttccagcccg tctcgtcagc gtaaccgctg ccgtcaaaca ggtcgcccgc
     7741 atggtttcct gcggaagttc gggttgagcc gtattaaggt ccgtgaagcc gctatgcgcg
     7801 gtgaaatccc gggtctgaaa aaggctagct ggtaattgtc accaattgaa tcacgggagt
     7861 aaagacagat gagcatgcaa gatccgatcg cggatatgct gacccgtatc cgtaacggtc
     7921 aggccgcgaa caaagctgcg gtcaccatgc cttcctccaa gctgaaagtg gcaatcgcca
     7981 acgtgctgaa ggaagaaggt tttattgaag attttaaagt tgaaggcgac accaagcctg
     8041 aactggaact tactctgaag tatttccagg gcaaagctgt tgtagaaagc attcagcgtg
     8101 tcagccgccc aggtctgcgc atctataaac gtaaagatga gctgccgaaa gttatggcgg
     8161 gtctgggtat cgcagttgtt tctacctcta aaggtgttat gactgatcgt gcagcgcgcc
     8221 aggctggtct tggtggcgaa attatctgct acgtagccta atcggaggaa aaaatgtctc
     8281 gtgttgctaa agcaccggtc gttgttcctg ccggcgttga cgtaaaaatc aacggtcagg
     8341 ttattacgat caaaggtaaa aacggcgagc tgactcgtac tctcaacgat gctgttgaag
     8401 ttaaacatgc agataatacc ctgaccttcg gtccgcgtga tggttacgca gacggttggg
     8461 cacaggctgg taccgcgcgt gccctgctga actcaatggt tatcggtgtt accgaaggct
     8521 tcactaagaa gctgcagctg gttggtgtag gttaccgtgc agcggttaaa ggcaatgtga
     8581 ttaacctgtc tctgggtttc tctcatcctg ttgaccatca gctgcctgcg ggtatcactg
     8641 ctgaatgtcc gactcagact gaaatcgtgc tgaaaggcgt tgataagcag gtgatcggcc
     8701 aggttgcagc ggatctgcgc gcctaccgtc gtcctgagcc ttataaaggc aagggtgttc
     8761 gttacgccga cgaagtcgtg cgtaccaaag aggctaagaa gaagtaaggt aacactatgg
     8821 ataagaaatt tgctcgtatc cgtcgtgcga cccgcgcacg ccgcaagctc caggagctgg
     8881 gcgcaactcg cctggtggta catcgtaccc cgcgtcacat ttacgcacag gtaattgcac
     8941 cgaacggttc tgaagttctg gtagctgctt ctactgtaga aaaagctatc gctgaacaac
     9001 tgaagtacac cggtaacaaa gacgcggctg cagctgtggg taaagctgtc gctgaacgcg
     9061 ctctggaaaa aggcatcaaa gatgtatcct ttgaccgttc cgggttccaa tatcatggtc
     9121 gtgtccaggc actggcagat gctgcccgtg aagctggcct tcagttctaa ggtagaggtg
     9181 taagatggct cacatcgaaa aacaagctgg cgaactgcag gaaaagctga tcgcggtaaa
     9241 ccgcgtatct aaaaccgtta aaggtggtcg tattttctcc ttcacagctc tgactgtagt
     9301 tggcgatggt aacggtcgcg ttggttttgg ttacggtaaa gcgcgtgaag ttccagcagc
     9361 gatccagaaa gcgatggaaa aagcccgtcg caatatgatt aacgtcgcgc tgaataacgg
     9421 cactctgcaa caccctgtta aaggtgttca cacgggttct cgcgtattca tgcagccggc
     9481 ttccgaaggt accggtatca tcgccggtgg tgcaatgcgc gccgttctgg aagtcgctgg
     9541 ggttcataac gttctggcta aagcatatgg ttccaccaac ccgatcaacg tggttcgtgc
     9601 aactattgat ggcctggaaa atatgaattc tccagaaatg gtcgctgcca agcgtggtaa
     9661 atccgttgaa gaaattctgg ggaaataaac catggcaaag actattaaaa ttactcaaac
     9721 ccgcagtgca atcggtcgtc tgccgaaaca caaggcaacg ctgcttggcc tgggtctgcg
     9781 tcgtattggt cacaccgtag agcgcgagga tactcctgct attcgcggta tgatcaacgc
     9841 ggtttccttc atggttaaag ttgaggagta agagatgcgt ttaaatactc tgtctccggc
     9901 cgaaggctcc aaaaaggcgg gtaaacgctt gggtcgtggt atcggttctg gcctcggtaa
     9961 aaccggtggt cgtggtcaca aaggtcagaa gtctcgttct ggcggtggcg tacgtcgcgg
    10021 tttcgagggt ggtcagatgc ctctgtaccg tcgtctgccg aaattcggct tcacttctcg
    10081 taaagcagcg attacagccg aagttcgtct gtctgacctg gctaaagtag aaggcggtgt
    10141 tgtagacctg aacacgctga aagcggctaa cattatcggt atccagatcg agttcgcgaa
    10201 agtgatcctg gctggcgaag taacgactcc ggtaactgtt cgtggcctgc gtgttactaa
    10261 aggcgctcgt gctgctatcg aagctgctgg cggtaaaatc gaggaataag tagcagatgg
    10321 ctaaacaacc gggattagat tttcaaagtg ccaaaggtgg cttaggcgag ctgaaacgca
    10381 gactgctgtt tgttatcggt gcgctgattg tgttccgtat tggctctttt attccgatcc
    10441 ctggtattga tgccgctgta cttgccaaac tgcttgagca acagcgaggc accatcattg
    10501 agatgtttaa catgttctct ggtggtgctc tcagccgtgc ttctatcttt gctctgggga
    10561 tcatgccgta tatttcggcg tcgatcatta tccagctgct gacggtggtt cacccaacgt
    10621 tggcggaaat taagaaagaa ggggagtctg gtcgtcgtaa gatcagccag tacacccgct
    10681 acggtactct ggtgctggca atattccagt cgatcggtat tgctactggt ctgccgaata
    10741 tgcctggtat gcaaggcctg gtgattaacc cgggctttgc attctacttc accgctgttg
    10801 taagtctggt cacaggaacg atgttcctga tgtggttggg cgaacagatt actgaacgag
    10861 gtatcggcaa cggtatttca atcattatct tcgtcggtat tgtcgcggga ctcccgccag
    10921 ccattgccca tactatcgag caagcgcgtc aaggcgacct gcacttcctc gtgttgctgt
    10981 tggttgcagt attagtattt gcagtgacgt tctttgttgt atttgttgag cgtggtcaac
    11041 gccgcattgt ggtaaactac gcgaaacgtc agcaaggtcg tcgtgtttat gctgcacaga
    11101 gcacacattt accgctgaaa gtgaatatgg cgggggtaat cccggcaatc ttcgcttcca
    11161 gtattattct gttcccggcg accatcgcgt catggttcgg gggcggtact ggttggaact
    11221 ggctgacaac aatttcgctg tatttgcagc ctgggcaacc gctttatgtg ttactctatg
    11281 cgtctgcaat catcttcttc tgtttcttct acacggcgtt ggttttcaac ccgcgtgaaa
    11341 cagcagataa cctgaagaag tccggtgcat ttgtaccagg aattcgtccg ggagagcaaa
    11401 cggcgaagta tatcgataaa gtaatgaccc gcctgaccct ggttggtgcg ctgtatatta
    11461 cctttatctg cctgatcccg gagttcatgc gtgatgcaat gaaagtaccg ttctacttcg
    11521 gtgggacctc actgcttatc gttgttgtcg tgattatgga ctttatggct caagtgcaaa
    11581 ctctgatgat gtccagtcag tatgagtctg cattgaagaa ggcgaacctg aaaggctacg
    11641 gccgataatt ggtcgcccga gaagttacgg agagtaaaaa tgaaagttcg tgcttccgtc
    11701 aagaaattat gccgtaactg caaaatcgtt aagcgtgatg gtgtcatccg tgtgatttgc
    11761 agtgccgagc cgaagcataa acagcgccaa ggctgatttt ttcgcatatt tttcttgcaa
    11821 agttgggttg agctggctag attagccagc caatcttttg tatgtctgtg cgtttccatt
    11881 tgagtatcct gaaaacgggc ttttcagcat ggaacgtaca tattaaatag taggagtgca
    11941 tagtggcccg tatagcaggc attaacattc ctgatcataa gcatgccgta atcgcattaa
    12001 cttcgattta tggcgtcggc aagacccgtt ctaaagccat cctggctgca gcgggtatcg
    12061 ctgaagatgt taagatcagt gagctgtctg aaggacaaat cgacacgctg cgtgacgaag
    12121 ttgccaaatt tgtcgttgaa ggtgatctgc gccgtgaaat cagcatgagc atcaagcgcc
    12181 tgatggatct tggttgctat cgcggtttgc gtcatcgtcg tggtctcccg gttcgcggtc
    12241 agcgtaccaa gaccaacgca cgtacccgta agggtccgcg caaaccgatc aagaaataat
    12301 cggggtgatt gaataatggc aaaggcacca attcgtgcac gtaaacgtgt aagaaaacaa
    12361 gtctctgacg gcgtggctca tatccatgct tctttcaaca acaccatcgt gactatcact
    12421 gatcgtcagg gtaacgcgtt gggttgggca acagccggtg gttccggttt ccgtggttct
    12481 cgcaaatcca ctccgtttgc agctcaggtt gcagcagagc gttgcgctga cgccgtgaaa
    12541 gaatacggca tcaagaatct ggaagttatg gttaaaggtc cgggtccagg ccgcgaatct
    12601 actattcgtg ctctgaacgc cgcaggtttc cgcatcacta acattactga tgtgactccg
    12661 atccctcata acggttgtcg tccgccgaaa aaacgtcgcg tataacgcct cgttttccag
    12721 gtttgttgga gaaagaaaat ggcaagatat ttgggtccta agctcaagct gagccgtcgt
    12781 gagggcaccg acttattcct taagtctggc gttcgcgcga tcgataccaa gtgtaaaatt
    12841 gaacaagctc ctggccagca cggtgcgcgt aaaccgcgtc tgtctgacta tggtgtgcag
    12901 ttgcgtgaaa agcaaaaagt tcgccgtatc tatggtgtgc tggagcgtca gttccgtaac
    12961 tactacaaag aagcagcacg tctgaaaggc aacaccggtg aaaacctgtt ggctctgctg
    13021 gaaggtcgtc tggacaacgt tgtataccgt atgggcttcg gtgccactcg tgcagaagca
    13081 cgtcagctgg ttagccataa agcaattatg gtaaacggtc gtgttgttaa catcgcttct
    13141 tatcaggtta gtccgaatga cgttgtaagc attcgtgaga aagcgaagaa gcagtctcgc
    13201 gtgaaagccg ctctggagct ggctgagcag cgtgaaaagc caacttggct ggaagttgat
    13261 gctggcaaga tggaaggtac gtttaagcgt aagccggagc gttctgatct gtctgcggac
    13321 attaacgaac acctgatcgt cgagctttac tccaagtaaa gcttagtacc aaagagagga
    13381 cacaatgcag ggttctgtga cagagtttct aaaaccgcgc ctggttgata tcgagcaagt
    13441 gagttcgacg cacgccaagg tgacccttga gcctttagag cgtggctttg gccatactct
    13501 gggtaacgca ctgcgccgta ttctgctctc atcgatgccg ggttgcgcgg tgaccgaggt
    13561 tgagattgat ggtgtactac atgagtacag caccaaagaa ggcgttcagg aagatatcct
    13621 ggaaatcctg ctcaacctga aagggctggc ggtgagagtt cagggcaaag atgaagttat
    13681 tcttaccttg aataaatctg gcattggccc tgtgactgca gccgatatca cccacgacgg
    13741 tgatgtcgaa atcgtcaagc cgcagcacgt gatctgccac ctgaccgatg agaacgcgtc
    13801 tattagcatg cgtatcaaag ttcagcgcgg tcgtggttat gtgccggctt ctacccgaat
    13861 tcattcggaa gaagatgagc gcccaatcgg ccgtctgctg gtcgacgcat gctacagccc
    13921 tgtggagcgt attgcctaca atgttgaagc agcgcgtgta gaacagcgta ccgacctgga
    13981 caagctggtc atcgaaatgg aaaccaacgg cacaatcgat cctgaagagg cgattcgtcg
    14041 tgcggcaacc attctggctg aacaactgga agctttcgtt gacttacgtg atgtacgtca
    14101 gcctgaagtg aaagaagaga aaccagagtt cgatccgatc ctgctgcgcc ctgttgacga
    14161 tctggaattg actgtccgct ctgctaactg ccttaaagca gaagctatcc actatatcgg
    14221 tgatctggta cagcgtaccg aggttgagct ccttaaaacg cctaaccttg gtaaaaaatc
    14281 tcttactgag attaaagacg tgctggcttc ccgtggactg tctctgggca tgcgcctgga
    14341 aaactggcca ccggcaagca tcgctgacga gtaaccggat cacaggttaa ggttttactg
    14401 agaaggataa ggtcatgcgc catcgtaaga gtggtcgtca actgaaccgc aacagcagcc
    14461 atcgccaggc tatgttccgc aatatggcag gttcactggt tcgtcatgaa atcatcaaga
    14521 cgactctgcc taaagcgaaa gagctgcgcc gcgtagttga gccgctgatt actcttgcca
    14581 agactgatag cgttgctaat cgtcgtctgg catttgcccg tactcgtgat aacgagatcg
    14641 tggcaaaact gtttaacgaa ctgggcccgc gtttcgcgag ccgtgccggt ggttacactc
    14701 gtattctgaa gtgtggcttc cgtgcaggcg acaacgcgcc gatggcttac atcgagctgg
    14761 ttgatcgttc agagaaagca gaagctgctg cagagtaatc tgaagcaatg taaaaaaacc
    14821 cgccccggcg ggttttttta tacccgtagt atccccactt atctacaata gctgtactct
    14881 ttttgttcat cccctggagt atttatgtgg ttacttgacc agtgggcaga gcgccatata
    14941 gcagaagcgc aagcgaaagg tgagtttgat aacctggcag gtagcggcga accattgata
    15001 ctggatgatg attctcacgt gccaccggaa ttacgtgcgg ggtatcgctt gctgaagaat
    15061 gccggttgct taccgccaga acttgagcaa cggagagaag caattcagct tctggatatt
    15121 ctcaaaggta tccgtcacga tgatccgcaa tatcaagagg ttagccgtcg attgtcatta
    15181 ctggaattga agctgcgaca agctggattg agtaccgatt ttttacgcgg cgattatgct
    15241 gacaagttgt tggacaaaat caacgataac tagtggagta tgtatgtatc gcattggtga
    15301 gctggcaaaa atggcggaag taacacctga cacgattcgt tattacgaaa aacagcagat
    15361 gatggagcat gaagtgcgta ctgaaggcgg gtttcgcctg tataccgaaa gcgatctcca
    15421 gcgattgaaa tttatccgcc atgccagaca actaggtttc agtctggagt cgatccgcga
    15481 gttgctgtcg atccgcatcg atcctgaaca ccatacctgt caggagtcaa aaggcattgt
    15541 gcaggaaaga ttgcaggaag tcgaagcacg gatagccgag ttgcagagta tgcagcgttc
    15601 cttgcaacgc cttaacgatg cctgttgtgg gaccgcccat agcagtgttt attgctcgat
    15661 tcttgaagct cttgaacaag gggcgagtgg cgttaagagt ggctgttgat tttttgcact
    15721 gacaggatta cactcgcgcc gttaaataac caactggagt ttttatgagc cgatatcagc
    15781 atactaaagg gcagataaag gataatgcga tagaagcatt actacatgat cccttattcc
    15841 gacagcgagt agagaaaaat aagaaaggga aaggcagtta catgcgaaaa ggaaaacatg
    15901 gcaatcgggg gaactgggag gccagtggca agaaagtaaa tcactttttt accactggtc
    15961 ttctgctttc aggcgcttgt taagagcggt tattctgctc tttcagcaaa tcacgaattt
    16021 ctgccagtaa tacttcttct ttagttggtg caggtgcggc agctggttct tcttttttcc
    16081 gattcagttt gttgattagc ttaatcgcca taaagatggc aaaggccaca atcagaaaat
    16141 caaagacgtt ttgaatgaag acaccgtaat gcatcacaac agcagggata tccccctgcg
    16201 catcgcgtag cgtgacagca aactgtttaa agtcgatccc gccaattaat aagcccagag
    16261 gtggcatgat gatatcggca accagtgaag atacaatctt cccgaatgcc gcaccgataa
    16321 tgacacccac cgccaaatcc accacgttcc cgcgcatcgc aaattcgcga aattctttaa
    16381 taatgctcat gttattctcc ctatgaagcc gacaaccata agtctaacaa atgttaagcc
    16441 attttcctgc caggaatgaa tttaattaat ccttgataat caaagggaaa taataaggcg
    16501 tcattagacg ccttattaat tacaagaaga aagggcttgg ctggaagagt ctttcgacgt
    16561 cggtaataaa ctttttatct gtgaggaaca taattacgtg atcgccttgc tcaatgcgca
    16621 gattgtcatt ggcaatcatc acgtcgtttc cacgtaccac cgctccaata atcgttcctg
    16681 gcggtagctt gatttcgtca atgactctgc cgacaacgcg tgaggtgctt tcatcaccgt
    16741 gagcaacggc ttcaatagct tctgctacgc cgcggcgcaa tgaggaaaca ccaacaatat
    16801 ctgcttttcg cacatggcta agcaacgcag aaatagttgc ttgttgtggt gaaattgcaa
    16861 tatcgatcac gctcccctga accagatcca cataagcgcg acgctggatc aataccatca
    16921 cctttttcgc acccatacgt ttggcaagca tggcggacat gatattggcc tcgtcatcgt
    16981 tggtgacggc aataaacaga tcaacttgat cgatatgttc ttcggccagc agttcttgat
    17041 ccgacgcatc accaaaaaag acgatcgtat tctgcaactt ttccgccagt tcggcagcgc
    17101 gctgctgatt acgttcgatg agtttaacgc tgtaatcttt ttccagacga cgcgccagcc
    17161 ctgcaccgat attaccgcca ccaaccagca tgatccgctt atacggtttt tccagtcgct
    17221 gtaattcact catcaccgcg cggatatgct gtgaagcggc aataaagaac acttcatcac
    17281 cagcttcaac aatggtcgaa ccttgtggac gaatggggcg atcgtggcgg aaaattgctg
    17341 ccacacgagt atcgatatgt ggcatatgtt cgcgcatggt cgaaagtgca ttaccaatca
    17401 gcgggccgcc ataataggct ttaactacag cgaggctgac tttaccctca gcgaagttca
    17461 ccacctgcaa tgcgcctgga tactcaatca gtcggtaaat attatcgata accaactgct
    17521 ctggtgcgat cagatgatca atcggtacag catctgaatg aaatagctta tcggcatcgc
    17581 gtacgtagtc tggtgagcgg atgcgagcga tgcgattagg ggtgttgaaa agtgagtagg
    17641 ctacctggca ggcaaccata ttggtttcat ctgaactggt tacagcaacc agcatatcgg
    17701 cgtcgtcggc acctgcctcc cgcaatacgc gtggatgaga gccatgcccc tgcacgactc
    17761 gcaggtcaaa tttatcctgc aaggtccgca gacgctcacc gttggtatcg acaacagtaa
    17821 tatcgttgtt ctcgccaacc aggttttccg ccagtgtgcc gccaacctgg ccggcaccca
    17881 gaatgataat tttcatcagt ctcgacccgt tatctcatca ctttttgatt agcttagcgt
    17941 aaaagaagcc gtcgccctct tcggcaccag gcagattttg tttacccggt tgctctggtg
    18001 ttcctgtttc gcaaagttcg gcatcagcgg tacgttgcaa aaaagcttta atctgcaggc
    18061 tattctcttc cggtaacacc gaacaggtgg catagaccag ggttccaccg gattttaaat
    18121 gcggccaaat ggcgtcgaga atttcagact gcaattgcgc gagttccggg atatcgcgat
    18181 cgcgacgtaa ccatttaata tccggatggc gacgaatcac ccccgttgct gaacaaggcg
    18241 catctaataa aatgcgatca aactgttgct cgccacacca ttgggaaggg taacggccat
    18301 caccttgttt cacggtcgcc ttcataccaa ggcgttttaa attgtcgtga acgcgagaga
    18361 ggcgctgttc gtcaatatca accgcaacaa cctgcgcttc tggtgccacc tcaaggatat
    18421 gcgttgtttt accgccgggg gcggcacaaa gatccagaat gtgttcaccg ttttgtggcg
    18481 caagccaggt catgcaacct tgtgctgatg catcctgaac ggtaacccat ccgtcttcaa
    18541 aaccaggtag cgcatgaaca ggtgcaggtg tttccagccg tacagcatca gggtaatccg
    18601 catgcgggaa acctttcatt cctgcttcat ccaacaatgc aagccagctg tcgcgggaat
    18661 gatgcgtacg attaacgcgc agccacattg gcggacgctg gttattggct tcgacgatgg
    18721 attgccactg ctctggatac gctttttgca gacgcttcag caaccaggaa ggatgcagat
    18781 aacgtgcatc actggtatta aactcggcta ataactcttc ttgctgacgc tggaactggc
    18841 gtaatacacc gttaatcaac cctttaagtt gcggacgctt aattgcgata gcgccttcaa
    18901 ccgtttcagc cagcgcagca tgaggtggaa tgcgggtata aagcagttga tacaaaccaa
    18961 ccataatcag gtaatgcaca gtgcgctgtt tgccggtcat cggacgggcc attaacttat
    19021 taatcagcca gtctaactgc gaaagcgtac gcagtacgcc aaagcacaac tcttgaagaa
    19081 gtgctttgtc tttatcagaa actttttgct ggagcggtgg cagaatgttg cttaatgatt
    19141 gcccttgctc gacgacttgt tcaacggcct gggccgccat gctacgtaaa ttacgttgtt
    19201 ttttcataag tataaaaatg cccggtaaga ccgggcttag aagagtggac tatcaggcca
    19261 gacggttgcc cggaacaaac cattcccgac gagaattcag gaggtcttgc gcgctcatcg
    19321 ctttcttacc cgcaggttgt aacgagagca ggttcaggat gccatcacca gtcgcaacct
    19381 gaatgccttg tttgttggct tcaaggatcg ttcctggtgc agcgttggtt gccgtatcaa
    19441 tgaccgacgc tttccagact ttaaccggtt gtccttcaat ttccagccag ctcattggcc
    19501 atgggttgaa agcgcgaatg cagcgttcaa gctgcgctgc cgaaagcgac cagtcaatac
    19561 gcgcttcttc tttactcaac ttctcggcgt aagtgacaag agtttcgtcc tgaacttctg
    19621 gtttcgccgt gccgtctgct agttgtttca acgtggtgat tagcccttgt gggccaagct
    19681 ctgccagctt gtcgtacagc gtaccactgg tatcttctgc agtaatcggg caggagagct
    19741 tatagagcat atcaccggtg tctaaaccga catccatttg cataatggtc acaccagttt
    19801 ctgcatcacc cgcccatagt gagcgttgga ttggtgcagc accgcgccag cgtggcagca
    19861 gtgaaccatg aacgttgata cagccaagac gcggcatctc cagcactgct ttaggcagaa
    19921 ttaagccata ggcgacgacg accataacat cagcctgcag atcggcgacc agttgctggc
    19981 tttcttgtgg acgcagggaa acaggttgaa aaacgggcag acctttttcc tcagccagaa
    20041 ctttaaccgg gctgggcatc agttttttac cgcgtcctgc cggtcggtct ggctgggtga
    20101 acacgccaac gacgttatga ccagaagaca acagcgcgtc gagatgacgc gctgcaaagt
    20161 caggtgtacc cgcaaaaata atacgtagtg attctgacac gttagttctt atccttaagc
    20221 ccgggctttc agacgatcta gtttttcaac tttctgacga atacgttgtt gtttcagcgg
    20281 tgacagataa tccataaaca gtttgccgac caggtgatcc atctcatgct gaatacagat
    20341 ggctaacagc ccctctgctt ccagttcaaa tggtttaccg tcgcggtcaa gggcgcgaat
    20401 tttaactttc tctgcgcgcg gcactaaagc acgttgttca gggatcgaca ggcaaccttc
    20461 ttcaatgcct gtttcgccgc ttttttctaa aagctccgga ttgattaaca ccagccgttc
    20521 gtcacggttt tccgaaacat caataacaat gatacgttga tggatatcaa cctgggttgc
    20581 cgccaggcca ataccttctt ctgcgtacat cgtctcgaac atatcatcga cgatacgctg
    20641 tatttctgca ttcacttctt ctaccggttt agcaactttg cgaagccgct cgtccggaat
    20701 atgtaacact tgcaaaactg acataaatct ccagagatgt gttcaggggt tagaaagatt
    20761 atttcttcta ttctagacaa atccccctct gattgacagc atcactgacc aatcgcaaag
    20821 attgctaagg ctgcttatgg cagggagata aggatggtcg atacagaaat ttggctgcgt
    20881 ttaatgagta tcagcagctt gtacggcgat gatatggtcc gtatagctca ctggctggca
    20941 aaacagtcgc aaattgatgc ggttggattg cagcaaacag ggcttacatt gcggcaggca
    21001 caacgctttc tttcatttcc gcgaaagagt atcgaaagct cactttgttg gttggagcaa
    21061 cccaaccatc atttaatccc tgcggacagc gaattttatc ctcctcaact tctggtgacg
    21121 acagattacc ccggcgcact gtttgttgaa ggagaactgc acgcgctgca ttcatttcag
    21181 cttgccgtag tggggagtcg ggcgcattca tggtatggcg agcgatgggg acggttattt
    21241 tgcgaaactc tggcgacgcg tggagtgaca attacgagtg gactggcgcg tggaatcgat
    21301 ggtgtggcgc ataaagctgc cttacaggta aatggcgtca gcattgctgt attggggaat
    21361 ggacttaata ccattcatcc ccgccgccat gcccgactgg ctgccagtct gcttgaacat
    21421 ggcggagctc tcgtctcgga atttcccctc gatgttccac cccttgctta caatttccca
    21481 cgaagaaatc gcattatcag tggtctaagt aaaggtgtac tggtggtgga agcggctttg
    21541 cgcagtggtt cgctggtgac agcacgttgt gcgcttgagc agaggcgtga agtttttgcc
    21601 ttgccaggtc caatagggaa tccgggaagc gaagggcctc actggttaat aaaacaaggt
    21661 gcgattcttg tgacggaacc ggaagaaatt ctggaaaact tgcaatttgg attgcactgg
    21721 ttgccagacg cccctgaaaa ttcattttat tcaccagatc agcaagacgt ggcattgcca
    21781 tttcctgagc tcctggctaa cgtaggagat gaggtaacac ctgttgacgt cgtcgctgaa
    21841 cgtgccggcc aacctgtgcc agaggtagtt actcaactac tcgaactgga gttagcagga
    21901 tggatcgcag ctgtacccgg cggctatgtc cgattgagga gggcatgcca tgttcgacgt
    21961 actaatgtat ttgtttgaaa cctatattca cactgaagct gagttgcgtg tggatcaaga
    22021 caaacttgaa caggatctta ccgacgcagg ttttgagcga gaagatatct acaatgccct
    22081 gctatggctg gaaaaacttg ctgattatca ggaagggttg gcagaaccga tgcaactggc
    22141 ctcggatcct ctctccatgc gtatttatac accggaagag tgtgaacgac tggatgccag
    22201 ctgccgtggg tttctgcttt tccttgagca gattcaggtg ctcaaccttg aaactcgtga
    22261 aatggtgata gagcgagtgc tcgcgctgga taacgcagag ttcgaactgg atgatctgaa
    22321 atgggtgatc ctgatggtgt tgttcaacat cccggggtgc gaaaatgcgt accagcaaat
    22381 ggaagaatta ctctttgaag tgaatgaagg tatgctgcat taatcatttc tttaattcag
    22441 cataagttgt tatggcaaaa tcagcactgt tcacggtgcg taataatgag tcctgcccaa
    22501 agtgcggggc tgaactggtt attcgatccg ggaaacacgg tccgtttctt ggatgctcac
    22561 agtatccggc gtgtgactac gtccgtcttc tgaaatcttc agcggatgga catattgtca
    22621 aagttctgga ggggcaggtt tgccctgcat gtggcgcaaa tctggtatta cgccagggac
    22681 gctttggtat gtttattggt tgcagtaact accctgaatg cgaacatacc gaacttatcg
    22741 ataaaccgga cgaaacagca attacatgcc cccaatgtcg gacgggccat ctggtccagc
    22801 gccgctcccg ttatggcaaa acatttcact cctgtgatcg ctacccggag tgtcaatttg
    22861 ccattaactt caaaccaata gctggagaat gccctgagtg tcattatccg ctactcatcg
    22921 aaaagaaaac cgcgcagggt gtaaaacact tttgtgccag taaacaatgt ggaaagccgg
    22981 tttcggcgga ataataacgt gaataataac ctgcaaggag acgctatcgc agctgcgata
    23041 gatgtcctca atgaagaacg tgtcatcgcc tatccaacgg aagccgtttt cggtgttggg
    23101 tgcgatcctg atagcgaaac agcagtgatg agactgttgg agttaaaaca gcgtccggtt
    23161 gataaggggc tgattttaat cgcggcaaat tacgagcagc ttaaacccta tattgatgac
    23221 accatgctga ctgatgcgca gcgtgaaacc attttttccc gctggccagg tcctgtcacc
    23281 tttgtctttc ccgcgcctgc gacaacaccg cgctggttga cgggccgctt tgattcgctt
    23341 gctgtacgag tcaccgacca tccgttggtg gttgctttgt gccaggctta tggtaaaccg
    23401 ctggtttcta ccagtgccaa cttgagtgga ttgccacctt gtcgaacagt agacgaagtt
    23461 cgcgcacaat ttggcgcggc gttcccggtt gtgcctggtg aaacgggggg gcgtttaaat
    23521 ccttcagaaa tccgcgatgc cctgacgggt gaactgtttc gacaggggta acataatgga
    23581 aacctatgct gtttttggta atccgatagc ccacagcaaa tcgccattca ttcatcagca
    23641 atttgctcag caactgaata ttgaacatcc ctatgggcgc gtgttggcac ccatcaatga
    23701 tttcatcaac acactgaacg ctttctttcg tgctggtggt aaaggtgcga atgtgacggt
    23761 gccttttaaa gaagaggctt ttgccagagc ggatgagctt actgaacggg cagcgttggc
    23821 tggtgcagtt aataccctca agcggttaga agatggacgc ctgctgggtg acaataccga
    23881 tggtgtaggc ttgttaagcg atctggaacg tctgtctttt atccgccctg gtttacgtat
    23941 tctgcttatc ggcgctggtg gagcatctcg cggcgtacta ctgccacttc tttccctaga
    24001 ctgtgcggtg acaatcacta atcggacggt atcccgcgcg gaagagttgg ctaaattgtt
    24061 tgcgcacact ggcagtattc aggcgttggg tatggacgaa ctggaaggtc atgagtttga
    24121 tctcattatt aatgcaacat ccagtggcat cagtggtgat attccggcga tcccgtcatc
    24181 gctcattcat ccaggcattt attgctatga catgttctat cagaaaggaa aaactccttt
    24241 tctggcatgg tgtgagcagc gaggctcaaa gcgtaatgct gatggtttag gaatgctggt
    24301 ggcacaggcg gctcatgcct tccttctttg gcatggcgtt ctgcctgatg tagaaccggt
    24361 catcaaacta ttgcaacagg aattgtcagc gtgaatcagg ccatccagtt tccggatagg
    24421 gaagagtggg acgagaataa aagatgtgta tgttttcctg ctctcgtgaa tggtatgcaa
    24481 ctgacatgtg cgatctctgg cgagagtctg gcgtatcgct ttactggagg tacgccagaa
    24541 cagtggttag cgagttttcg tcagcatcgc tgggacctgg aagaagaagc ggaaaactta
    24601 attcaggaac aaagtgaaga tgatcaaggt tgggtctggt taccttgatc cagatattcg
    24661 tccttccatt tcacgtagtt atttgcggaa tagcgtaacc cagccttctc ttcatcactt
    24721 aacgggcgga tctgtttgac gggactaccg agatacagat atccgctctc cagccgttta
    24781 ttttgtggga ccaggctacc cgcaccaatc atcacatcat cttctactat tgcgccatca
    24841 agtaaaattg agcccatccc aaccaaaact cgattgccaa tggtgcagcc gtggagcatc
    24901 accttgtgac caacagtgac atcttcgcca atggttaatg ggttgccatc tgggttgtac
    24961 gaggatttat gagtgacatg caacatactg ccatcctgga tattggtgcg tgctccgatc
    25021 tgtacataat gtacatctcc acgaatcaca acgagcggcc agatccccac atcatcagcc
    25081 agacgaacgt caccaatcac gacgctgcta tcgtcgatca ttacgcgctg gccgatttgt
    25141 ggaaaaagat cgcggtatgg gcgtaaaaca tcagacatac tcacctcaac aataaatgat
    25201 ttcctaatga ctttgggtgc attattagcc ttgtgcaagt cttttagtat gcaaaaaagc
    25261 accgttttgt gtgcgaatgc agcaaaaagg gtgaaaaaac aacaaacaga aaaaaagatc
    25321 aaaaaagtac ttgtgcaaaa aattgggatc cctataatgc gcctccgttg agacgacaac
    25381 gtgaaacact tcacaggatg gtcggaacaa cgaagagaaa aaattctgaa attcagggtt
    25441 gactctgaaa gaggaaagcg taatatacgc cacctcgcga cagtgagctg aaag
//
LOCUS       DAJTZL010000041        25204 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.041, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000041 DAJTZL010000000
VERSION     DAJTZL010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25204
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.041"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..849)
                     /gene="melB"
                     /locus_tag="OO544_001530"
     CDS             complement(<1..849)
                     /gene="melB"
                     /locus_tag="OO544_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HCR6767156.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQPSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTL"
     gene            complement(952..2307)
                     /gene="melA"
                     /locus_tag="OO544_001531"
     CDS             complement(952..2307)
                     /gene="melA"
                     /locus_tag="OO544_001531"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HCR6767157.1"
                     /translation="MMTAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALQDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
                     YARYLHIKQVGLCHSVQGTAEELARDLNIDPSTLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            2590..3498
                     /gene="melR"
                     /locus_tag="OO544_001532"
     CDS             2590..3498
                     /gene="melR"
                     /locus_tag="OO544_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HCR6767158.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGNITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            3697..5964
                     /gene="adiA"
                     /locus_tag="OO544_001533"
     CDS             3697..5964
                     /gene="adiA"
                     /locus_tag="OO544_001533"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HCR6767159.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGASGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLSVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            6289..7050
                     /gene="adiY"
                     /locus_tag="OO544_001534"
     CDS             6289..7050
                     /gene="adiY"
                     /locus_tag="OO544_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HCR6767160.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            7187..8524
                     /gene="adiC"
                     /locus_tag="OO544_001535"
     CDS             7187..8524
                     /gene="adiC"
                     /locus_tag="OO544_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HCR6767161.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            8628..10271
                     /gene="eptA"
                     /locus_tag="OO544_001536"
     CDS             8628..10271
                     /gene="eptA"
                     /locus_tag="OO544_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HCR6767162.1"
                     /translation="MLKRLLKSPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWLKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQQAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPGQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDVLQTCRRVSE"
     gene            10268..10936
                     /gene="basR"
                     /locus_tag="OO544_001537"
     CDS             10268..10936
                     /gene="basR"
                     /locus_tag="OO544_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HCR6767163.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYTCDGVTTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            10937..12037
                     /gene="pmrB"
                     /locus_tag="OO544_001538"
     CDS             10937..12037
                     /gene="pmrB"
                     /locus_tag="OO544_001538"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HCR6767164.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISIFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQYVANQI"
     gene            complement(12013..12102)
                     /gene="pmrR"
                     /locus_tag="OO544_001539"
     CDS             complement(12013..12102)
                     /gene="pmrR"
                     /locus_tag="OO544_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HCR6767165.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATY"
     gene            complement(12214..13716)
                     /gene="proP"
                     /locus_tag="OO544_001540"
     CDS             complement(12214..13716)
                     /gene="proP"
                     /locus_tag="OO544_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HCR6767166.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDDIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(13980..14846)
                     /locus_tag="OO544_001541"
     CDS             complement(13980..14846)
                     /locus_tag="OO544_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HCR6767167.1"
                     /translation="MTKTLLDGPGRVLESVYPRFLVDLAQGDDARLPQAHQQQFRERL
                     MQELLSRVQLQTWTNGGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVVLLLGDLREKATQHLAESINAAPTTR
                     HYYHQWFASSGGEHADFLSWLGKWSTADKQPVCWSVTQRWQTVALGMPRLCSAQRLVG
                     AMVEEIFSVNLA"
     gene            complement(14843..17071)
                     /gene="crfC"
                     /locus_tag="OO544_001542"
     CDS             complement(14843..17071)
                     /gene="crfC"
                     /locus_tag="OO544_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HCR6767168.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELSKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARHELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDALNAQLAALFQD
                     DSEPLSEIRTCCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGVHFPVFHGVVPHVNTRQLFSEVISRQDATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFCAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHGWIYTDAQLLRDDIQTLFTAE
                     RY"
     gene            17484..17819
                     /locus_tag="OO544_001543"
     CDS             17484..17819
                     /locus_tag="OO544_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phnA family protein"
                     /protein_id="HCR6767169.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            17977..18420
                     /gene="yjdN"
                     /locus_tag="OO544_001544"
                     /pseudo
     CDS             17977..18420
                     /gene="yjdN"
                     /locus_tag="OO544_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418531.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
     gene            18553..19341
                     /gene="phnC"
                     /locus_tag="OO544_001545"
     CDS             18553..19341
                     /gene="phnC"
                     /locus_tag="OO544_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HCR6767170.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVALNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTREQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQQFDNERFDHLYRSINRIEENAKAA"
     gene            19366..20382
                     /gene="phnD"
                     /locus_tag="OO544_001546"
     CDS             19366..20382
                     /gene="phnD"
                     /locus_tag="OO544_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6767171.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLSPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEIQGVKSNNGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            20508..21287
                     /gene="phnE"
                     /locus_tag="OO544_001547"
     CDS             20508..21287
                     /gene="phnE"
                     /locus_tag="OO544_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HCR6767172.1"
                     /translation="MQTITIAPPKRSWFSLLSWAIVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            21308..22033
                     /gene="phnF"
                     /locus_tag="OO544_001548"
     CDS             21308..22033
                     /gene="phnF"
                     /locus_tag="OO544_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HCR6767173.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGIGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            22034..22486
                     /gene="phnG"
                     /locus_tag="OO544_001549"
     CDS             22034..22486
                     /gene="phnG"
                     /locus_tag="OO544_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HCR6767174.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLNALNITADYEVIRTAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHAERCALIDALM
                     QQSRHFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            22483..23067
                     /gene="phnH"
                     /locus_tag="OO544_001550"
     CDS             22483..23067
                     /gene="phnH"
                     /locus_tag="OO544_001550"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HCR6767175.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALYQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLATPLSNDIVNQSLRFHTNAPLVSQPEQATFAVTDEAIS
                     SEQLNALSTDTAVAPEAGATLILQVASLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            23067..24131
                     /gene="phnI"
                     /locus_tag="OO544_001551"
     CDS             23067..24131
                     /gene="phnI"
                     /locus_tag="OO544_001551"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HCR6767176.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLTKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEQNHG"
     gene            24124..24969
                     /gene="phnJ"
                     /locus_tag="OO544_001552"
     CDS             24124..24969
                     /gene="phnJ"
                     /locus_tag="OO544_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HCR6767177.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTECTDDAT
                     LIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKSQ"
     gene            24966..>25204
                     /locus_tag="OO544_001553"
     CDS             24966..>25204
                     /locus_tag="OO544_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR6767178.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIHYENRSLYAMSEADRRRL"
BASE COUNT         6226 a         6889 c         6346 g         5743 t
ORIGIN      
        1 taacgtcacc aggttagcag ctcccgcata cgacagataa taggggaaca aatccgcatc
       61 accgataaca tatgagaaat aatagatagc aaatccggta ataatgttgc tggctacatt
      121 ataagcaaga gccataccca agaggcatga aagctgatcg tttttataaa ttagcgcaac
      181 gatggcttta agtgtcagat ggcttccttc agcagacggt tgattgtctg acgaaaagac
      241 ttcatgcaca ttgcgcagag tgatgatggt tgaaacaata aaaaaggcga tcagtaccag
      301 tgtgaacatc tgaaagccaa atccccgatc gccaccgccg acataattaa caaatggtag
      361 cgtcacacct gccgtaacaa agcccgccag actggcaaaa aaacgcggat aaggaaccag
      421 ttgttcacgc tcacgtttat cgagcgtgat ggttggaacc agcgaccaga agggaatatc
      481 cataatggtg taagtcatgc cccagaggat gtaggtcacg caaacaaaga caatctgagt
      541 agtaccttca aacagatgcg cactaaagag gagaaataag attacagagt ttgccaacgt
      601 accgatcagg atccagggtt tgaacttacc ccatcgcgat cgcgtagcat ttacaatcca
      661 tcccataatc ggatcgttaa tagcatccca gatcctcgcc accagaaaca aagtaccgac
      721 caaacccaca gacagcccaa cgacatcggt gtagtaatac atgaggtaca tatacacaat
      781 gccgatcgca aaatccttcc cgaacgctcc aaatccataa ctgagttttg tagtcattga
      841 aatgctcata gggtatcggg tcgctgttac caacgcccgc cttctgttat tggtatcgtc
      901 agaggtgtgc gccggaataa atcccccggc gcagtagcgt ttagtcgcgt tttaacggtg
      961 caaccagcct ggcagccagt cgccgtgggc ggcaatcagg tcgtcaacaa gagcatatat
     1021 ttcgtcaatg cccagcacgg cggcagtatg cgggtccatc atcgcggcgt ggtaaacacg
     1081 gtcgcgattt tccgtaagaa tagcttcggt cagcagcgtc tgtacattga tgttggtttg
     1141 catcagggcg gccagatgcg aaggtagcgt accgacttta gtcggctgaa tgccattagc
     1201 atcaaccaga caggctactt ccacgcaaca tccttgtggc aggttatcaa tcaaaccatc
     1261 gttacggacg ttgccgtaaa tcacactcgg ctcgccagtc cagatagcgt tcatgattgt
     1321 gctggcatat tctcgtgacg gtttaatatc aatccgggag gcgtttttat actcctccag
     1381 ctctttatgc cagttcgcca gctgctcgac gcagcgtttc gggtactcat ccagcggtac
     1441 tttataacgc tcaatcaaat cctcacgacc tggcttaata aaccacggcg tgtattcagc
     1501 aaaatgttct gacgattccg taacgaagta gcccagcttt ttgaacattt cgtagcgcac
     1561 aatattctgg cagcgagtat taccatgaat atttggcttc ggtgcctgcc ctgcttcata
     1621 agccgccagc agttccgggt agagattcac gtaactgccg tcggcggttt tacgctccag
     1681 ttccaggtaa aacgccatat ggttgatacc agcgcaacgg taacgcagcg tggacggatc
     1741 gatattgagg tcacgcgcca gctcttccgc cgttccctgc accgaatggc acagccctac
     1801 ctgtttgata tgcagatagc gggcatacat cgcccaggta ttcatcgcca ttgggttaac
     1861 gtagttgagc atggtggcat cggggcagac ttccgtcatg tcctcgcaaa tttgccacag
     1921 atgcggaatg gtacgtagcg cgcgcataat accgcccggc cccaacgtat cggcaatggt
     1981 ttgttccaga ccatgccgct tacagacctc gaaatcagtc accgtgcaag gttcataacc
     2041 gccaatctga aatgccacca cgacaaaatc ggcatcctgt aaggcttctt tctgttgggt
     2101 gtggcaggtg attttgccgc tggcccctgc tgaatccatc agcttacgca ccacaatatg
     2161 cgactcttcc aggcgggtgg gatcaatgtc catcagggca atatgcgccg ttttcagcgc
     2221 ctcgcgatgg aacacatcac caagaatatt tttaacgaaa atcgtcgaac cagcgccgat
     2281 aaatgtaatt ttgggtgcag tcatcatgca gatctcctgg cttgcttgaa taacttcatc
     2341 atggcaggcg aatctgcaga aaacctccgg cttccaggtt gagcattccc ataaactcag
     2401 atcctgcgtg aagcagcagt aaatctgagt ttatggtaat aagttacctg aaaagcagag
     2461 ttttcggcct catgatgctc ggacgctccc ctcgcctctc tgggaagagg gtgaaaaccg
     2521 tgctcccact cgcagtcatc ctccctcact cctgccataa ttctgatatt ccaggaaaga
     2581 gagccatcca tgaatacaga tacgtttatg tgcagcagcg acgaaaaaca gacccgcagc
     2641 ccgctgtcgc tgtattcgga ataccagcgg atggagattg agtttcgcgc accgcatatc
     2701 atgcccacca gccactggca tggtcaagtc gaagtgaatg tgcctttcga tggcgatgtg
     2761 gaatacctga tcaacaatga aaaagtgaat atcaatcagg gtaatatcac gctgttctgg
     2821 gcctgtacac cgcaccaact aacagatacc ggaacctgtc agagcatggc gatttttaat
     2881 ctgccgatgc atctgtttct ctcctggccg ctggataaag acctgattaa ccacgtcact
     2941 cacggcatgg tgatcaaatc actggccaca cagcaactta gcccgtttga agtgcgccgc
     3001 tggcagcagg aattaaacag tccgaacgag caaattcgcc agctcgccat tgatgaaatt
     3061 ggcctgatgc tcaagcgatt tagcctctct ggctgggaac cgattctggt caataaaacc
     3121 tcgcgcacac acaaaaacag cgtctcgcgc catgcgcaat tttatgttag ccagatgctg
     3181 ggctttattg ccgaaaacta tgatcaggcg ctgaccatca acgatgtggc tgagcacgtc
     3241 aaacttaacg ccaactatgc aatggggata ttccagcggg tcatgcaatt gacgatgaaa
     3301 cagtacatta ccgcgatgcg catcaaccac gttcgcgcgt tactgagcga taccgataaa
     3361 agtattctcg atattgccct gacggcaggc tttcgttcaa gtagtcgttt ttacagcacg
     3421 ttcggcaaat atgtcggcat gtcgccgcaa caataccgca aacttagcca acagcgccgc
     3481 cagacgtttc ccggctaaag acgatatcag tatcagccaa aaaaatagtt tagccgtcga
     3541 taataagcag tgaaaataat ttaaaataat cacataacgc attgtttata aaaataaata
     3601 tttcaaaaat gtttgttttt cacgcgcttt acagcccgaa aaggccggaa gatacttgcc
     3661 cgcaacgaag attccttcat aaccgggtaa gcaatgatga aagtattaat tgttgaaagc
     3721 gagtttctcc atcaagacac ctgggtcggt aacgccgttg agcgtctggc agatgcttta
     3781 agccagcaaa atgttaccgt gattaaatcc acctcctttg atgatggttt tgccattctc
     3841 tcttcaaacg aagccattga ctgcctgatg ttcagctatc aaatggaaca tccggacgaa
     3901 catcaaaacg tcagacaatt gatcggtaag cttcatgagc gccaacaaaa cgtgccggtc
     3961 ttcctgttgg gcgatcggga aaaagccctc gccgcaatgg atcgcgacct gctggagctt
     4021 gtcgatgaat tcgcctggat tctggaagat accgccgact ttatcgccgg acgcgctgtt
     4081 gccgcgatga cccgctaccg ccagcagctg ttgccgccac tgttcagcgc gctgatgaaa
     4141 tatagcgaca tccatgaata ttcctgggca gcgccaggcc accagggcgg cgttggtttt
     4201 accaaaacac ccgccggacg tttctaccat gactactatg gtgaaaatct gttccgcacc
     4261 gatatgggca tcgaacgaac ttccctcggt tctttgcttg accatactgg cgcatttggc
     4321 gaaagcgaaa aatatgccgc acgcgtattt ggtgccgatc gctcctggtc ggtagtcgtc
     4381 ggtgcttccg gctctaaccg caccatcatg caggcttgca tgaccgataa cgatgtcgtg
     4441 gtcgttgacc gtaactgcca taaatccatc gaacaaggct tgatgctgac aggcgcgaaa
     4501 ccggtctaca tggtgccaag ccgcaaccgc tacggcatta tcgggccaat ctatccgcag
     4561 gaaatgcaac ctgaaacctt gcagaagaaa atcagtgaaa gcccgctgac taaagacaaa
     4621 gccgggcaaa aaccgtctta ctgcgtggtg accaactgca cctatgacgg cgtgtgttat
     4681 aacgctaaag aagcgcagga tctgctggaa aaaacctccg atcgtctgca ctttgacgaa
     4741 gcctggtacg gctatgcacg tttcaacccg atctatgccg atcactatgc catgcgcggc
     4801 gaacctggcg atcacaacgg tcctaccgtt ttcgccaccc actccaccca caaactgctg
     4861 aatgcgcttt cacaggcttc ttatattcat gtacgtgaag gtcgtggggc gattaacttc
     4921 tcccgcttca accaggccta catgatgcat gccaccacct ccccgctgta tgccatctgc
     4981 gcatccaacg acgtggcggt gtcgatgatg gacggcaaca gcggcctgtc actgacgcag
     5041 gaagtgatcg acgaagcggt tgatttccgt caggcgatgg cgcggctgta taaagagttc
     5101 accgctgacg gtagctggtt cttcaaaccg tggaacaaag aagtcgtcac cgacccacaa
     5161 accggcaaaa cctatgactt tgctgacgca ccaaccaaac tgctgaccac cgttcaggac
     5221 tgctgggtaa tgcatccggg cgaaagctgg cacggcttca aagatattcc ggataactgg
     5281 agtatgctcg acccgattaa agtcagcatc cttgctccgg gaatgggtga agatggtgaa
     5341 ctggaagaaa ccggtgttcc ggcggcgctg gtcactgcct ggcttggtcg ccacggcatt
     5401 gtgcctaccc gcaccactga cttccaaatt atgttcctgt tctctatggg cgtaacccgt
     5461 gggaaatggg gaactctggt taacaccctt tgctccttca aacgccacta tgacgccaac
     5521 acaccgctgg cgcaggtgat gccggaactt gttgaacaat atcctgacac ttacgcgaac
     5581 atggggattc acgatctggg tgacaccatg tttgcctggc tgaaagaaaa caaccctggc
     5641 gcacggttga acgaagccta ttccggcctg tcggtggcgg aaatcacccc gcgtgaagcg
     5701 tacaacgcga ttgtcgacaa caatgtcgaa ctggtatcca ttgaaaatct gccaggacgc
     5761 atcgcggcaa actcagttat cccatatccg ccaggaatcc cgatgctgct gtctggtgaa
     5821 aacttcggcg ataaaaacag tccgcaagta agttatttac gctcgctgca atcctgggac
     5881 caccatttcc ctggatttga acacgaaact gaagggactg aaattattga cggtatttac
     5941 cacgttatgt gtgtgaaagc gtaaccacta ttccgctgaa ggcgtaattg tttaaataac
     6001 attacgccgc ctggccttag gccttttgag tatggcaacg ttttcataaa aattgctgca
     6061 aacaaaaatg tcatactttt tgcgcggccc caccccgcgc ttttttttgc ctgttattta
     6121 tcctgtaaaa aaatatgtac atgagaaatt actataaaaa tttgtaatat tagtaaaact
     6181 cgttattttt atgcatgttt atattcatca tacaattata taaccatttc ccggtatcgc
     6241 tttgctttag cgagaactgg tcttttattc gcactcagga gtacatgtat gaggatttgc
     6301 agcgaccaac cttgtattgt tttattgact gaaaaagatg tctggataag ggtgaatggg
     6361 aaagaaccta ttagccttaa agctaaccat atggcgttat taaattgtga aaataatatt
     6421 atcgacgtct cctctcttaa caacactttg gttgctcata ttagtcacga catcatcaaa
     6481 gattacctcc ggtttctgaa taaagatctc tcgcaaatac cagtatggca acgtagcgct
     6541 acgcccatac tcaccctgcc atgcctgacg ccagacgtct ttcgcgttgc cgcgcaacac
     6601 agcatgatgc ccgcagaaac tgagtcagaa aaggaacgaa cacgcgcatt attattcacg
     6661 gtgctatccc gttttctcga cagtaaaaaa ttcctttcat taatgatgta tatgttacgt
     6721 aattgtgtaa gtgacagcgt ttatcaaatt attgaaagcg atattcacaa agactggaat
     6781 cttagtatgg tagccagttg tttatgtctt agcccaagtc tgttaaagaa aaagctgaaa
     6841 agcgaaaaca ccagttatag ccaaataatc accacctgcc gcatgcgtta tgccgtaaat
     6901 gaattaatga tggacggtaa aaatatctct caggtatcac agtcctgcgg ctacaacagt
     6961 acgtcgtact ttatttctgt ctttaaagac ttctacggta tgacgccgct gcattatgtt
     7021 agtcagcaca gagaacgaac tgtcgcctga tttttaacct taacgaagag ctatattaat
     7081 aacggcatca gcgataaccc ggtcgataat aattcaacta tcgaatgcag gcgtatgata
     7141 tgacgtaatt tattgtcacg aagctcgcct tcgcaggagt ttaattatgt cttcggatgc
     7201 tgatgctcac aaagtgggct taatccccgt caccctgatg gtgtcgggga atattatggg
     7261 gtcaggtgtt tttctgttac ctgcaaacct ggcctccact ggcgggattg ccatttatgg
     7321 atggttggtg acgattatcg gtgcgctggg gctctcgatg gtatacgcca aaatgtcgtt
     7381 cctcgatcca agtcctggtg gttcttacgc ttacgcccgc cgctgctttg gcccgtttct
     7441 cggttatcaa accaacgtcc tctactggct ggcctgctgg atcggcaata tcgctatggt
     7501 ggtcattggc gtaggatatt taagttactt cttcccgatt ctgaaagatc cattggtatt
     7561 aaccatcacc tgcgtcgtgg tgctgtggat cttcgtcctg ctgaacattg tcggtccgaa
     7621 aatgatcacc cgtgtgcagg ccgttgccac cgtgctggcg ctgatcccca tcgtcgggat
     7681 tgccgtattt ggctggttct ggttccgtgg tgaaacctat atggcggcat ggaacgtcag
     7741 cggcctgggc accttcggtg cgattcaaag tacccttaac gttacgctgt ggtcgttcat
     7801 cggtgtggaa agtgcctccg ttgccgcagg tgtggtgaaa aacccgaaac gcaatgtccc
     7861 tatcgccacc attggtgggg tattgattgc cgccgtttgc tatgtgctct ccaccaccgc
     7921 gattatgggg atgatcccta atgccgcact gcgcgtttct gcctcgccat tcggtgatgc
     7981 cgcacggatg gcgttgggtg acaccgctgg ggccattgtt tccttctgcg cagctgcggg
     8041 ttgcttaggt tcactgggcg gctggacgtt gctggcgggt caaacggcga aagccgctgc
     8101 cgatgacgga ctgttcccac cgatttttgc ccgtgtaaat aaagcgggta cgccagtggc
     8161 ggggctgatt atcgtcggta ttttgatgac catcttccag ctcagtagca tttcgccaaa
     8221 cgcaaccaaa gagttcggtc tggtatcttc cgtgtcggtc atctttacac tggtgccata
     8281 tctttacacc tgtgcggcgt tactgctgct cggccacggt cactttggta aagcacgccc
     8341 ggcatatctg gctgttacta ccattgcctt cctctactgc atctgggccg tggtggggtc
     8401 cggggcgaaa gaggttatgt ggtcatttgt caccctgatg gtcatcaccg ccatgtatgc
     8461 gctgaattac aaccggctac ataaaaaccc gtatccctta gatgcaccaa taagcaaaga
     8521 ttaattcccc ttaatccagc aaacataaaa gccaacctta agaacttaag gttggcttaa
     8581 ttttgctttg cgagcatatg cgcactttgt tcgatggaaa caccgtgatg ttgaagcgcc
     8641 tactaaaaag cccctctttg aatttactcg cctggctatt gttggccgct ttttatatct
     8701 ctatctgcct gaatattgcc ttttttaaac aggtgttgca ggcgctgccg ctggattcgc
     8761 tgcataacgt actggttttc ttgtcgatgc cggtcgtcgc tttcagcgtg attaatattg
     8821 tcctgacact aagctctttc ttatggctta atcgaccact ggcctgcctg tttattctgg
     8881 ttggcgcggc tgcacaatat ttcataatga cttacggcat cgtcatcgac cgctcgatga
     8941 ttgccaatat tattgatacc actccagcag aaagttatgc gctgatgaca ccgcaaatgt
     9001 tattaacgct gggattcagc ggcgtgcttg ctgcgctgat tgcctgctgg ctaaaaatca
     9061 aacctgccac ctcgcgtctg cgcagtgttc ttttccgtgg agccaatatt ctggtttctg
     9121 tactgctgat tttgctggtc gccgcactgt tttataaaga ctacgcctcg ttgttccgca
     9181 ataacaaaga gctggtgaaa tccttaagcc cctctaacag cattgttgcc agctggtcat
     9241 ggtactccca tcagcgactg gcaaatctac cgctggtgcg aattggtgaa gacgcccacc
     9301 gcaacccgtt aatgcagaac gaaaaacgta aaaatttgac catcctgatt gtcggcgaaa
     9361 cctcgcgggc ggagaacttc tccctcaacg gctacccgcg tgaaactaac ccgcggctgg
     9421 cgaaagataa cgtggtctat ttccctaata ccgcatcttg cggcacggca acggcagttt
     9481 cagtaccgtg catgttctcg gatatgccgc gtgagcatta caaagaagag ctggcacagc
     9541 accaggaagg cgtgctggat atcattcagc aagcgggcat caacgtgctg tggaatgaca
     9601 acgatggcgg ctgtaaaggt gcctgcgacc gcgtgcctca ccagaacgtc accgcgctga
     9661 atctgcctgg tcagtgcatc aacggcgaat gctatgacga agtgctgttc cacgggctgg
     9721 aagagtacat caataacctg cagggtgatg gcgtgattgt cttacacacc atcggcagcc
     9781 acggtccgac ctattacaac cgctatccgc cgcagttcag gaaatttacc ccaacctgcg
     9841 acaccaatga gatccagacc tgtaccaaag agcaactggt gaacacttac gacaacacgc
     9901 tggtttacgt cgactatatt gttgataaag cgattaatct gctgaaagaa catcaggata
     9961 aatttaccac cagcctggtt tatctttctg accacggtga atcgttaggt gaaaatggca
    10021 tctatctgca cggtctgcct tatgccatcg ccccggatag ccaaaaacag gtgccgatgc
    10081 tgctgtggct gtcggaggat tatcaaaaac ggtatcaggt tgaccagaac tgcctgcaaa
    10141 aacaggcgca aacgcaacac tattcacaag acaatttatt ctccacgcta ttgggattaa
    10201 ctggcgttga gacgaagtat taccaggctg cggatgatgt tctgcaaact tgcaggagag
    10261 tgagtgaatg aaaattctga ttgttgaaga cgatacgctg ttattgcagg gactgattct
    10321 ggcggcgcaa accgaaggct acacgtgcga tggcgtaaca accgcgcgga tggcggaaca
    10381 aagccttgag gccggtcatt acagcctggt ggtactggat ttagggttac ccgacgaaga
    10441 tggactgcat tttctcgccc gtatccggca gaaaaaatac accctgccgg tactgatcct
    10501 caccgctcgc gatacgctga ccgacaaaat cgccgggctg gatgtcggtg ccgatgacta
    10561 tctggtgaag ccttttgcgc tggaagagtt acacgcccgt atccgcgccc tgctacgacg
    10621 ccataataat cagggcgaaa gtgagctgat tgttggcaat ctgacgctga atatgggtcg
    10681 ccgtcaggta tggatgggcg gtgaagagtt gattctgacg cccaaagaat atgctctgct
    10741 gtcacggtta atgctcaaag caggcagtcc ggtacatcgg gaaattctct acaacgacat
    10801 ctataactgg gacaatgaac cctcgaccaa caccctggaa gtgcatatcc acaatctgcg
    10861 cgacaaagtg ggcaaagccc gtatccgcac cgtgcgcggc tttggctata tgctggtcgc
    10921 gaatgaggaa aactaattga atctgatgcg ttttctgcgc cgaccaatat cgctgcgcca
    10981 acggctgata ttgaccatcg gggccatttt gttggtgttt gagctgatca gcatcttctg
    11041 gctatggcat gaaagtaccg agcagattca gctgtttgag caggcgctac gcgacaatcg
    11101 caacaacgat cgccacatca tgcgtgagat ccgcgaggcg gtcgccagcc tgattgtccc
    11161 cggcgtcttt atggtcagcc tgacgctatt tatctgctat caggcggtac gccgcatcac
    11221 ccgcccgctg gcggagctgc aaaaagagct ggaagcgcgc accgccgaca acttaacgcc
    11281 cattgccatt cacagcgcca ccctcgaaat cgaagcggtg gtttcggcgt tgaacgatct
    11341 ggtcagtcgc ctgacgagca cgctggataa cgaaaggtta tttaccgctg acgtcgcgca
    11401 cgaactgcga acgccactgg cgggggtgcg tttgcatctg gaactgctgg cgaaaacgca
    11461 tcacattgat gtagcaccgt tagtggcacg gcttgatcag atgatggaga gcgtctccca
    11521 gctgctgcaa ctggcgcgtg ccggacagtc attttcttcc ggtaattatc aacatgtaaa
    11581 actgctggaa gatgtcattc tcccctcgta tgacgaactc agtaccatgc tcgaccagcg
    11641 acagcaaacc ctgctattgc cagagagcgc cgccgacatc accgttcagg gtgatgcaac
    11701 cctgctgcgg atgttattgc gaaacctggt agaaaacgcc catcgttaca gcccacaagg
    11761 cagcaacatt atgattaagc tgcaagaaga tggcggagcg gtcatggccg ttgaagatga
    11821 aggaccgggt attgatgaga gtaaatgcgg ggagttgagt aaagcgtttg tacgtatgga
    11881 cagccgttat ggcgggattg gtctggggtt aagtattgtc agccgcatta cacagttgca
    11941 tcacgggcag tttttcctgc aaaaccggca agagacttcc ggcacgcggg cctgggtacg
    12001 gctgaagaaa gatcagtacg tagcaaacca gatataaaga aagctgctga ccaccagcac
    12061 actgaacacg gtagttaaac tttcataaac acggtttttc attgcactct cctccgttaa
    12121 cctggaggag agtatgcgcg tcagagatta agtgaacctt aagagttcaa ttggcctgat
    12181 aagacagcgt cacatcaggc catccgtttc agcttattca tcaattcgcg gatgttgctg
    12241 caccaggcgg gtacgtttcg cctgcaaatc ggcaatctcg tggtcaatat catcgatttt
    12301 ctgctcgata ttatcgtaat gctcgacgag aatttccttc gcttcctgta tatctgacgc
    12361 cgccggtgtc gcacctttca acggacgatt tgccgtctct ttcatggtta cgccggtgat
    12421 taaaccaatc accgccacta ccatcaggta ataggcaggc atcatcagat tctgcgagct
    12481 ttcgaccagc caggccgcca gtgttggcgt cagaccggca accagcaccg aaatattaaa
    12541 tgccgccgcc agcgcgctgt aacggatatg cgtcgggaac atcgctggca aggtagaagc
    12601 cataacgccc gtaaagcagt taaggatcac cgccagcatc agtaacccgg caaaaatcag
    12661 gccgatgacg ttactgttaa tcagaataaa cgccgggatc gccaacacaa acagggcaac
    12721 actaccaagt agcacaaacg gacgacggcc aaaacggtca ctcagcaagc ccatcaccgg
    12781 ctggacaaac agcataccga tcataatggc gataataatc agcaccccgt ggtcttcgga
    12841 gtaatgcagg ttatgcgaca aataactcgg catataggtc agcaacatgt agtaagtcac
    12901 gttggtggca attaccagac caatacatgt caacaggctg cgccagtatt tagtggcaat
    12961 ctctttaaac gagactttcg ggccatcctg caaaccttcg cggtcgccct gttccagttt
    13021 atcaacatgc tgctggaacg ccggagtttc ttccagcgca tggcgcaggt aaagcccgat
    13081 aatccctaac ggcagagcaa taaagaacgg aatacgccag ccccagtcga ggaagttcgc
    13141 ttcgccgaca atggtcgaaa ttaacaccac cacgcccgca cccagcacaa acccggcaat
    13201 agaaccgaag tccagccagc tgcccataaa gccacgttta cggtcagggg agtattccgc
    13261 aacaaatatc gacgccccgg tatattcacc gccgaccgag aaaccttgtg ccatcttaca
    13321 gatcaacagc agaatcggtg cccaaatacc aatcgtgtcg taggacggta ttaagccaat
    13381 acagaacgta ctgatcgaca taatcacaat agtgatagcg aggatcttct ggcgaccata
    13441 tttatcgccc aacataccaa agaagagccc gccaagcggt cgaatcagaa agggaacgga
    13501 gaaagtggca agtgcagcaa ccatctgcac gctggggtca gcccccggga aaaaaacttt
    13561 acctaatgcg taagcaacaa aaccataaac accaaaatcg aaccattcca ttgcattacc
    13621 cagtgatgct gcggtaatgg ctttacgcag tttaccgtca tcaataatgg tgacatcacg
    13681 aagggtaatc ggttttactt ttttcctttt cagcatagct ttcctcgcag agttggccca
    13741 tttccgcaaa cgccagaaaa atcagcgcga cggccgcaaa aggggagcgg gaattgcagg
    13801 atgcaatctc tgtaacaagc gtagcgggtt tgcttacacc ctccggttaa ggaatgtaca
    13861 atcaaaccag ttgacgcctg aattgggcag ttaatgacct ttttacccta ccagtgttta
    13921 aatttgtgat caacttcaca catggatttc gtttaggact cattgatgta actgattatt
    13981 tacgccaaat ttacagagaa gatttcctcg accattgccc ccaccaaacg ctgcgctgaa
    14041 cagagtcgtg gcatccccag cgcgactgtt tgccagcgtt gggtcactga ccagcaaacg
    14101 ggctgtttat ctgccgtgga ccactttccc agccaactta aaaaatcagc atgctcccct
    14161 cccgaagagg caaaccactg atggtaatag tgacgcgtgg tcggtgccgc attgatactc
    14221 tctgccagat gttgtgtcgc tttctcgcgc aaatcaccca gtaacaacac caccgcttgc
    14281 gggcttccca gacggcaggc atcgccaaat gcaccccagc gcaactcctc cagaacgata
    14341 aaacagcgcc caggcaacga ccaggcgtta tacgccccag cacgccagcg ggtaaaaatt
    14401 tgctcgctct gatcacctgc ctgcaccagt aggcctcgtt gcgttaacgc cttttccttc
    14461 cagcgacaac gggctgaaaa ccactcatac aaggcggcaa tttgctgggg gagctccggg
    14521 aaagcagagt gctggcggtg atccattttt tgcagcagcg ccagatgctg cgcgatctgc
    14581 gtcagcgcca gatgaccggg atccagcatc gacgccagtt tttccaccat tgtcagtcgc
    14641 aggctaagcg gcgcatttaa catgccgccg ttcgtccatg tctgaagctg cacacgcgaa
    14701 agaagttcct gcatcagtcg ttcacgaaac tgctgctgat gggcttgtgg aaggcgggca
    14761 tcatcgccct gcgccagatc cactaaaaag cggggataaa ccgactccag cacgcgaccg
    14821 gggccgtcca gtaacgtctt ggtcaatatc gttctgccgt gaaaagtgtt tgaatatcat
    14881 cgcgtaacag ctgggcgtcg gtgtaaatcc agccgtgagt catcacagtc tgctgcaatt
    14941 gctgctgcat cagcctgacc accgattcat tttgttgacg cagagccagg ctttcgcgta
    15001 aacgcgtctg taattccgtc aaacatgaag cgaactcagc gcaaaaagta ttcatgcctg
    15061 ccgtaacaga ttcatcgacc tgctctgcca gaactttagc catttgttgg caataaagat
    15121 cgacttctgc gcttaaagct cgttgcaaca cactgtaatc aaccgtctct gtcggggatt
    15181 tctcatttcc ccgtccccag tcgggctgat tcaaccagcg cgaaaaagtc tcacgcacaa
    15241 cgcctaaacg cgtgctctgc tcgtccgttg catcctggcg cgaaatgact tcactgaaca
    15301 gctggcgagt gttgacgtgg ggaactactc cgtgaaaaac aggaaaatga accccaggac
    15361 gaaaccctga ctcgctcaat tccattttga cttgttgctc aatgggccga ataacatcgg
    15421 ttaacacccg gcaaagggtg gattcaagct cggcaaaacg cagcgtaaag tcgcgactga
    15481 tggtgttctg cgccgtctgt aacagtgtct cacagcaggt acgaatctcg cttaacggct
    15541 ccgaatcatc ctgaaacaag gcggctaact gcgcattcag cgcatcttgc tgttgacgca
    15601 gaaagtggtt ggcggaggtc agggccagct cgatttcatg tttaatctcg ccgctcacct
    15661 gcgcctgatt gagttgcaat agctgcaaac tttcttcgac ctgatggata ttttgccgca
    15721 attgttcaca agcgacgttt aacccgtgcg cacgaaaatc caggtattcc cgcgcctgct
    15781 gcgcgtaatt caacagttta tgcgcagcag atcgcagagc atacaacgag gcgttagcgt
    15841 aagcggcatg aagcaacgcc tgaattggct gggcgaacag cgaatcttcc cacaactgat
    15901 cggcagcatg acgaatatgt tcgaggtccg ccagatcggc atgacgccag cgcctgccga
    15961 gcgcggcatg ggcaaaatct tccacccagc gttgttgctc tggcgctggt aacttaccgt
    16021 tgttggctaa ctcatggcgc gctcgatttg ccaagtagcc ccacatcgac gacaccggaa
    16081 atatctgctg tggcgtaata cagcctttca tcagcgtccc ggaaatcagt gcccgcacct
    16141 ggtcggcgtc gtcactgtta cgatcctgtt gatcgaactt attgaccagc acatacagtg
    16201 gcaccgattg ccccaccgcc aaaatcgcct cacggacctc ttcatcggag atcgatttca
    16261 gttgcgtata atccagcacc gccagtaccg ccgaggcgcg tgccagctgc tggttaagca
    16321 ttttttgcag atgcggttgc ccggcttcat ttggcccggg ggtatccagt aacgtcaact
    16381 gaccgggata actctccagc cccgccagat ggacaaactc cacttcaatc acgggaatat
    16441 gctcaatggc ggcgtaagca gaaaaaggaa aatcgacgtc cagcgccttc gccagtcgca
    16501 ctaaatcatt caaacttttc agacaatgaa aaataggctg ggcacccaga taatattttt
    16561 cgaaagcgac gccattttcg atccgctgca taagcgcacg catatcttta tctatttcca
    16621 gcacatcggt cagatgctta atatcgcaat cacgcaggcg ctgttgtaat tgttgaatta
    16681 aacaatcgat tggcgcgaca tgtgaaaaat gcagtaccgg ttccttttgc ccgggcgtat
    16741 ggcgaataag cgtcggcagc gcagtcattg ggcgattacg attaggcaga acctccgtac
    16801 caacaatggc attaatggtg gttgatttcc ctgctttcat agtaccgaca atcgccagca
    16861 ccatttccag tcgggaaatt ttactcaact cattattcag catcgcgtga cgttcggcga
    16921 tattaggctg actccagggt aaagccagtt gtggcgcgtc gtctccgggt acagagagag
    16981 gcattttttc cagtaactgc aactgttggc gagaaagctg taacaggcgt tcagcctcct
    17041 gacttaactc atacagggtc tgtgtgtaca tagaaaattc ttccttaaag caaattttgt
    17101 tacttttatt tagccagatt gtttttgagt tctgtttgag ttctgttttt ggcttttata
    17161 attactgcaa gaaataattt tatatttagt gtgttgtttt ttatcagaat aaataacgtc
    17221 ttctgatgcg tttaaaacgt cagaaagata aaaatatcat gtgaataaaa aaaagaacaa
    17281 gtagagcatt aacattatct taaataataa atagaggcaa aaagattatt ttctttttgc
    17341 gtttcctttc aaatgaaaac gatcgtcgtc taaaatcagc agtacccccg acaaactcag
    17401 ggattttgtg tataattgcg gcctttttcg gcaatctgcc gttttttggc gcttttgccc
    17461 tgctgacttt tgaggaaatc cacatgtcat taccacactg cccaaaatgc aactccgaat
    17521 acacttacga agataacggc atgtacatct gcccggaatg tgcctacgaa tggaacgacg
    17581 cagaacctgc acaggaaagc gacgagctga tcgttaaaga tgctaacggc aatctgctgg
    17641 ctgacggcga cagcgttacc atcattaaag atctgaaggt gaaaggtagc tcttcgatgc
    17701 tgaaaattgg caccaaagtg aaaaacatcc gcctggttga aggcgaccat aacatcgatt
    17761 gcaaaatcga cggttttggt ccgatgaaac tgaaatctga gtttgtgaaa aagaactgat
    17821 tgtattgtga tcggtaagcc ggataaggcg ctcgcgccgc atccggcaac gatgccgact
    17881 gcctgatgcg acgcttgcgc gtcttatcag gcagcctctc atctgtataa atttcgaact
    17941 acacttaatt ggcttctctt aactgaggtc accatcatgc cgttaagtcc ctacctctct
    18001 tttgccggta actgtgccga cgcaattgct tattatcaac gtacgttggg cgctgaactg
    18061 ctctataaaa tcagcttcgg cgaaatgcca aaatcagcgc aggacagcgc cgagaactgc
    18121 ccttccggaa tgcaatttcc cgataccgcc atcgcccacg ccaatgtgcg cattgccgga
    18181 agcgacatca tgatgagcga tgccatgccg tcaggaaaag ccagctactc cggctttacg
    18241 ctggtgctcg attcgcaaca ggtcgaagaa ggaaaacgct ggtttgacaa tcttgccgct
    18301 aacggaaaaa tcgaaatggc ctggcaggaa actttctagg cgcatggctt tggcaaagtc
    18361 accgataaat ttggcgtacc gtggatgatt aatgtcgtca aacaacaacc aacgcaataa
    18421 cccgccggga ggcccgccct cccgcactgt catcgaattc ccgttaactc ttcatctgtt
    18481 agtcactttt aattaaccaa atcgtcacaa taatccgcca cgatggagcc acttttttag
    18541 ggaggctgca tcatgcaaac gattatccgt gtcgagaagc tcgccaaaac cttcaatcag
    18601 catcaggcgc tgcatgcggt tgctctgaac attcatcacg gtgaaatggt ggctctgctt
    18661 gggccgtcgg gttccggcaa atccaccctt ttacgtcact taagcggttt gattaccggc
    18721 gataaatccg ccggcagcca tatcgagctg ctgggccgca cagtccagcg cgaaggccgt
    18781 ctggcgcgcg atatccgcaa aagccgcgcc aacaccggct acatcttcca acaattcaac
    18841 ctggtgaacc gcctgagcgt actggagaac gtgctgattg gcgcgctcgg cagcacgccg
    18901 ttctggcgca cctgttttag ctggtttacc cgcgagcaga aacaacgcgc gttacaggcg
    18961 ctgacccgcg ttggcatggt gcattttgcc catcaacgcg tttccaccct ctccggcgga
    19021 cagcagcagc gtgtggcgat tgcccgcgcg ctgatgcagc aggcgaaggt gattctggcc
    19081 gatgaaccca tcgcctcgct ggacccggaa tccgcccgca tcgtgatgga caccctgcgc
    19141 gacatcaatc agaacgacgg catcaccgtg gtcgtcacgc tgcatcaggt ggattacgcc
    19201 ctgcgctact gcgaacgcat cgtcgccctg cgccaggggc acgttttcta cgacggcagc
    19261 agccaacagt ttgataacga acgttttgac catctctacc gcagcattaa tcgcatcgaa
    19321 gagaacgcga aagctgcctg acatccccat cattgaggaa aacgaatgaa cgctaagata
    19381 attgcctcgc tggccttcac cagcatgttc agcctcagca ccctgttaag cccggcacac
    19441 gccgaagagc aggaaaaggc gctgaatttc ggcattattt caacggaatc acagcaaaac
    19501 ctgaaaccgc aatggacgcc attcttacag gatatggaga agaagctggg cgtgaaggtg
    19561 aacgccttct ttgccccaga ctacgcaggc attatccagg gaatgcgctt caataaagtg
    19621 gatatcgcct ggtacggcaa cctgtcggca atggaagcgg tggatcgcgc caacggccag
    19681 gtcttcgccc agacggtcgc ggcggatgga tcgccaggtt actggagcgt gttgatcgtc
    19741 aacaaagata gtccgatcaa caacctgaac gatctgctgg cgaagcggaa agatctcacc
    19801 ttcggcaatg gcgatcctaa ctccacctct ggcttcctcg tccccggtta ctacgtcttt
    19861 gccaaaaaca atatctccgc cagcgacttt aagcgcaccg tcaacgccgg gcatgaaacc
    19921 aacgcgctgg ccgtcgccaa caagcaggtg gatgttgcca ccaacaacac cgaaaacctc
    19981 gacaagctga aaacctccgc gccagagaag ctgaaagaac tgaaggtgat ctggaagtcg
    20041 ccgctgatcc caggcgatcc gatcgtctgg cgcaagaatc tctccgaaac caccaaagac
    20101 aagatctacg acttctttat gaactacgga aaaacgccgg aagagaaagc ggtgctggaa
    20161 cgcctgggct gggcaccgtt tcgcgcctcc agcgacctgc aactggtgcc gattcgccag
    20221 ctcgcactgt ttaaagagat tcagggcgta aaaagcaata acggactgaa tgagcaggac
    20281 aagctggcga aaaccaccga gattcaggcg cagctggatg acctggaccg cctgaacaac
    20341 gcgttaagcg cgatgagttc ggtgagtaaa gcggtgcagt aatgtgctcg atcggtcccc
    20401 tctcccttga gggagagggt tagggtgagg gggaaatgcg cacgctgatg ccctcacccc
    20461 ggccctctcc cacagggaga gggagaaaac attaaacgga gccaaacatg caaaccatca
    20521 ccatcgcccc acccaagcgc agctggttct cgcttctgag ctgggccatt gttctcgccg
    20581 tgttggtcgt ctcgtggcag ggcgcggaaa tggccccgct tacgctgatc aaagacggcg
    20641 gcaacatggc gaccttcgcc gccgacttct tcccgcccga tttcagccag tggcaggatt
    20701 acctcaccga aatggccgtc acgctgcaaa tcgccgtctg gggcaccgcg ctggcagtgg
    20761 ttctctccat ccccttcggc ctgatgagcg ccgaaaacct ggtgccgtgg tgggtttacc
    20821 agcccgttcg ccgcctgatg gacgcctgcc gcgccattaa cgaaatggtc ttcgccatgc
    20881 tgttcgtggt cgccgtcggt ctcggaccgt tcgctggcgt gctggcgcta tttattcaca
    20941 ccaccggcgt gctctcgaaa ctgctttccg aagcggtgga agcgattgaa cccggcccgg
    21001 tggaaggcat tcgcgccacc ggcgccaaca agctggaaga gatcctttac ggcgtgctgc
    21061 cacaggtaat gccgctgctg atctcctact ccctctatcg cttcgaatcc aacgtccgct
    21121 cggcgaccgt cgtcggcatg gtcggtgcag gcgggatcgg cgtcaccctg tgggaagcga
    21181 ttcgcggttt ccagttccaa caaacctgcg ccctgatggt gcttatcatc gtcacggtca
    21241 gcctgctgga tttcctctct caacggttgc gtaagcactt tatctgataa gtgaggcatt
    21301 gatatctatg cacttgtcta cacatccgac cagctaccca acacgctatc aggagatagc
    21361 cgcaaaactt gagcaggagc ttcgtcaaca ctatcgctgc ggcgactatc ttcccgccga
    21421 gcagcaactg gcggcgcgct ttgaggtaaa ccgccacacc ctgcgccgcg ccatcgacca
    21481 actggtggaa aaaggctggg tacagcgccg tcagggcatc ggcgtgctgg tgctgatgcg
    21541 cccgttcgat tacccgctca acgcccaggc gcgttttagc cagaacctgc tggaccaggg
    21601 cagccatccc accagcgaaa aactgctgtc ggtattgcgc cccgcgtccg gtcacgttgc
    21661 tgacgcgctg gggattaccg agggtgagaa cgttatccat ttgcgcaccc tgcgtcgcgt
    21721 caacggcgtc gcgctctgtt taatcgacca ctactttgcg gacctcaccc tctggccgac
    21781 actgcaacgt ttcgacagcg gttcgctgca cgattttctg cgcgagcaaa ccggaattgc
    21841 gctgcgccgc agccagacgc ggatcagcgc ccgccgcgcc caggccaaag agtgccagcg
    21901 ccttgaaatc ccgaatatgt cgccgctgct gtgcgtgcgc acccttaacc accgtgacgg
    21961 tgaaagcagc ccggcggagt actccgtcag cctgacgcgc gccgacatga ttgaattcac
    22021 tatggagcac tgaatgcacg cagataccgc gacccgccag cactggatgt ccgtactggc
    22081 gcacagccaa ccggctgaac tggcagcccg cctgaacgcg ctgaacatca ccgccgacta
    22141 tgaagtgatc cgcactgccg aaactggcct ggtgcagatt caggcgcgaa tgggcggtac
    22201 cggcgaacgc ttttttgccg gcgacgccac gctgacccgt gccgccgtac gcctgactga
    22261 cggcaccctc ggctacagct gggtgctggg gcgtgataaa cagcacgccg aacgctgcgc
    22321 gctgattgat gcgctgatgc agcaatctcg ccactttcaa aacttatcag aaacccttat
    22381 tgccccgctg gacgctgacc gtatggcacg cattgccgca cgccaggccg aagtgaacgc
    22441 cagccgggtc gacttcttta cgatggttcg cggagacaac gcatgaccct ggaaaccgct
    22501 tttatgcttc ccgtgcagga tgcccagcac agttttcgtc gcctgttaaa ggccatgagc
    22561 gagccgggcg tgattgtcgc cctgtatcag ctcaaacgcg gctggcagcc gctgaatatc
    22621 gccaccacca gcgtgctgct gacgctggcc gataacgaca cgccggtgtg gcttgccacg
    22681 ccattaagta acgatatcgt caatcagagc ctgcgttttc ataccaacgc gccgctggtc
    22741 agccagccgg aacaggcgac cttcgcggtg acggatgagg cgatttccag cgaacagctc
    22801 aacgcccttt ccaccgacac cgccgttgcg ccggaagcgg gcgcgacgct gattttacag
    22861 gtcgccagcc tgagcggcgg acgcatgttg cgtctcaccg gcgcgggcat tgccgaagaa
    22921 cgaatgatcg ctccgcagct gccagagtgc attctgcacg aactcaccga gcgcccgcac
    22981 ccgttcccgc tcggcatcga cctaatcctg acctgcggcg agcgcctgct ggctatcccg
    23041 cgaaccactc atgtggaggt gtgctgatgt acgttgccgt gaaagggggc gaaaaggcga
    23101 tcgacgccgc ccacgccctg caagagagcc gacgccgggg cgataccgat ttgcccgaac
    23161 tgagcgtcgc ccagattgaa cagcagctta acctcgcggt ggatcgcgtg atgacagaag
    23221 gcggcattgc cgaccgcgaa ctggcggcgc tggcgctgaa acaggccagc ggcgataacg
    23281 tcgaagcgat tttcctgcta cgcgcctacc gcaccacgct gacgaaactg gcggtaagcg
    23341 agccgctcga caccacggag atgcgtctcg aacggcgtat ttcagcggtt tataaagata
    23401 ttcccggcgg ccagctgctt ggcccaacct acgactacac ccatcgcctg ctcgatttca
    23461 ctctgctggc aaacggcgaa gcgccgacgc tgaccaccgc cgacagcgaa caacagccgt
    23521 cgccgcacgt tttcagcctg ctggcgcgtc aggggctggc gaagtttgaa gaggatagcg
    23581 gagcacagcc ggatgacatc acccgcacgc cgccggttta cccctgttca cgttcttccc
    23641 gtttgcagca gttaatgcgc ggcgacgaag gctatttgct ggcgctggct tactccaccc
    23701 agcgcggtta cgggcgcaac cacccgttcg cgggcgagat ccgcagtggt tacatcgacg
    23761 tgtcgattgt gccggaagag ctgggatttg cggtaaacgt cggcgaactg ctgatgaccg
    23821 agtgtgaaat ggtcaacggt tttatcgacc cgccggatga gccgccgcac ttcacgcgcg
    23881 gctacgggct ggtattcggc atgagcgagc gcaaagcgat ggcaatggcg ctggttgacc
    23941 gtgcgttgca agccccggaa tacggcgagc acgcgacagg cccggcgcag gatgaagagt
    24001 tcgtgctggc gcatgccgac aacgtcgaag ccgcaggctt tgtctcgcac ctcaaactcc
    24061 cgcactacgt cgatttccag gccgaactgg agctactcaa acgtctgcaa caggagcaga
    24121 accatggcta atctgagcgg ctacaacttt gcctacctcg acgagcagac caaacgcatg
    24181 atccgccgcg ccatcttaaa agcggtggcg atccccggtt atcaggtacc gtttggcggg
    24241 cgcgagatgc cgatgccgta cggctgggga actggtggta tccagctcac cgccagcgtg
    24301 attggcgaaa gcgacgtgtt gaaggtgatt gaccagggtg cggatgacac caccaacgcc
    24361 gtgtcgattc gcaacttctt taagcgcgtg accggggtaa acaccactga atgtacggac
    24421 gatgcgacgc ttatccagac gcgtcaccgc atccccgaaa cgccgctgac cgaagatcag
    24481 atcattatct tccaggtgcc aatcccggaa ccgctgcgct ttatcgagcc gcgcgaaacg
    24541 gagacccgca ctatgcacgc gctggaggag tacggcgtga tgcaggtgaa actgtatgaa
    24601 gatatcgccc gcttcggtca tatcgccacc acctatgctt atccggtgaa ggtgaacggg
    24661 cgctacgtga tggacccgtc gccgatcccg aaattcgata acccgaaaat ggacatgatg
    24721 cccgccctgc agctgttcgg cgcagggcgc gagaagcgca tctatgcggt gccgccgttt
    24781 acccgcgtgg aaagtctcga tttcgacgat cacccgttca ccgttcagca gtgggatgag
    24841 ccatgcgcca tctgcggatc gacccacagc tatcttgatg aagtggtgct ggatgacgcc
    24901 ggaaaccgca tgtttgtctg ctccgatacc gattattgcc gccaacagag cgaggcgaaa
    24961 agccaatgaa tcaaccgtta ctttcggtca ataacctgac ccacctttac gcgccgggca
    25021 aaggctttag cgatgtctct tttgatttat ggccggggga agtgctgggc attgtcgggg
    25081 aatccggctc cgggaagacc acgctgctga agtcgatctc cgcgcgtctg acgccgcagc
    25141 agggggagat tcactatgaa aaccgttccc tctatgcaat gagcgaggcc gaccgccgtc
    25201 gcct
//
LOCUS       DAJTZL010000042        25103 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.042, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000042 DAJTZL010000000
VERSION     DAJTZL010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25103
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.042"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            194..382
                     /locus_tag="OO544_001554"
     CDS             194..382
                     /locus_tag="OO544_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjR family protein"
                     /protein_id="HCR6767179.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            394..1146
                     /gene="bcsQ"
                     /locus_tag="OO544_001555"
     CDS             394..1146
                     /gene="bcsQ"
                     /locus_tag="OO544_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HCR6767180.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCDHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            1143..1307
                     /locus_tag="OO544_001556"
     CDS             1143..1307
                     /locus_tag="OO544_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000025864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
                     /protein_id="HCR6767181.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPR"
     gene            1500..3761
                     /gene="bcsA"
                     /locus_tag="OO544_001557"
     CDS             1500..3761
                     /gene="bcsA"
                     /locus_tag="OO544_001557"
                     /EC_number="2.4.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HCR6767182.1"
                     /translation="MNELPERVSHKTQHLDEKKELGHLSAGARRLILGIIVTFSLILA
                     LICVTQPFNPLAQFIFLMLLWGVALIVRRMPGRFSALMLIVLSLTVSCRYIWWRYTST
                     LNWDDPVSLVCGLILLFAETYAWIVLVLGYFQVVWPLNRQPVPLPKDMSLWPSVDIFV
                     PTYNENLNVVKNTIYASLGIDWPKDKLNIWILDDGGREEFRQFAQNVGVKYIARTTHE
                     HAKAGNINNALKYAKGEFVSIFDCDHVPTRSFLQMTMGWFLKEKQLAMMQTPHHFFSP
                     DPFERNLGRFRKTPNEGTLFYGLVQDGNDMWDATFFCGSYAVIRRKPLDEIGGIAVET
                     VTEDAHTSLRLHRRGYTSAYMRIPQAAGLATESLSAHIGQRIRWARGMVQIFRLDNPL
                     TGKGLKFAQRLCYVNAMFHFLSGIPRLIFLTAPLAFLLLHAYIIYAPALMIALFVLPH
                     MIHASLTNSKIQGKYRHSFWSEIYETVLAWYIAPPTLVALINPHKGKFNVTAKGGLVE
                     EEYVDWVISRPYIFLVLLNLVGVAVGIWRYFYGPPTEMLTVVVSMVWVFYNLIVLGGA
                     VAVSVESKQVRRSHRVEMTMPAAIAREDGHLFSCTVQDFSDGGLGIKINGQAQILEGQ
                     KVNLLLKRGQQEYVFPTQVARVMGNEVGLKLMPLTTQQHIDFVQCTFARADTWALWQD
                     SYPEDKPLESLLDILKLGFRGYRHLAEFAPSSVKGIFRVLTSLVSWVVSFIPRHPERS
                     ETAQPSDQALAQQ"
     gene            3772..6110
                     /gene="bcsB"
                     /locus_tag="OO544_001558"
                     /pseudo
     CDS             3772..6110
                     /gene="bcsB"
                     /locus_tag="OO544_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
     gene            6117..7223
                     /gene="bcsZ"
                     /locus_tag="OO544_001559"
     CDS             6117..7223
                     /gene="bcsZ"
                     /locus_tag="OO544_001559"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase"
                     /protein_id="HCR6767183.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVIA
                     PSDARKITTSEGQSYGMFFALAANDRVAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPIGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            7205..10697
                     /gene="bcsC"
                     /locus_tag="OO544_001560"
                     /pseudo
     CDS             7205..10697
                     /gene="bcsC"
                     /locus_tag="OO544_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsC"
     gene            10779..12767
                     /gene="hmsP"
                     /locus_tag="OO544_001561"
     CDS             10779..12767
                     /gene="hmsP"
                     /locus_tag="OO544_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HCR6767184.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPIMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFAHPLPIEIFEESYLEEK"
     gene            12950..14236
                     /gene="dctA"
                     /locus_tag="OO544_001562"
     CDS             12950..14236
                     /gene="dctA"
                     /locus_tag="OO544_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HCR6767185.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            14456..15952
                     /locus_tag="OO544_001563"
     CDS             14456..15952
                     /locus_tag="OO544_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCR6767186.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNIGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAAATSVATDDVHPEVTDIPPAQ"
     gene            complement(16048..16977)
                     /gene="kdgK"
                     /locus_tag="OO544_001564"
     CDS             complement(16048..16977)
                     /gene="kdgK"
                     /locus_tag="OO544_001564"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HCR6767187.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVCLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            17209..17976
                     /gene="pdeH"
                     /locus_tag="OO544_001565"
     CDS             17209..17976
                     /gene="pdeH"
                     /locus_tag="OO544_001565"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HCR6767188.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFVEHDLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPMETLNTAVLAL"
     gene            18046..20106
                     /locus_tag="OO544_001566"
     CDS             18046..20106
                     /locus_tag="OO544_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HCR6767189.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWFKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIATNIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPTAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(20288..21610)
                     /locus_tag="OO544_001567"
     CDS             complement(20288..21610)
                     /locus_tag="OO544_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HCR6767190.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVASI
                     LGASVAPYIAAWLQTNYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(22021..23034)
                     /gene="yhjD"
                     /locus_tag="OO544_001568"
     CDS             complement(22021..23034)
                     /gene="yhjD"
                     /locus_tag="OO544_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HCR6767191.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(23083..23982)
                     /locus_tag="OO544_001569"
     CDS             complement(23083..23982)
                     /locus_tag="OO544_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6767192.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPSGI
                     AKNLLLPRLSEFLYQHPGIELELSSHNRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAMVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGQLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEYLD"
     gene            24502..>25103
                     /locus_tag="OO544_001570"
     CDS             24502..>25103
                     /locus_tag="OO544_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR6767193.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
BASE COUNT         5894 a         6365 c         6991 g         5853 t
ORIGIN      
        1 ttagcttgtg aatcaattta gaaggtcaca agacgtgtga ttttagtctt ttcgttttta
       61 actttttctg ttgcgtaacg gaaagtcaaa aagtgagcaa attcccgtct cgccgtaaac
      121 aataaaccgg cgaaatgctc acggttagaa ttgtttcatg aacggtacgg ttatttcata
      181 gggatcaagc aaaatgaata acaatgaacc agatactctg cctgatcccg cgataggcta
      241 tatcttccag aatgatattg tggcgttaaa gcaggcattt tcactgcctg atattgatta
      301 tgccgatatt tcccaacgcg aacagttggc cgcggcatta aaacgctggc cgttgctggc
      361 agagtttgcg caacaaaagt aggggattgg tgaatggccg tactgggatt gcagggggtg
      421 cggggaggcg tggggacaac aaccatcacc gccgcattag cctggtcatt acaaatgttg
      481 ggagaaaatg tcctggtggt cgatgcctgc ccggacaact tgttgcgcct gtcatttaat
      541 gttgatttta cccaccgtca gggctgggcc agagcgatgc tggatggcca ggactggcgt
      601 gacgctgggt tgcgctacac ctcgcagctc gatttgctgc cttttggtca gttatccatt
      661 gaagaacaag aaaatccaca gcactggcaa acccggctga gcgatatttg ctccggctta
      721 cagcaactaa aagccagcgg gcgttaccag tggattttaa tcgacttacc gcgtgatgcc
      781 tcgcagataa cccaccagct gctgagtttg tgcgatcact cgctggcaat cgtcaatgtg
      841 gatgccaact gccatatccg actgcatcag caagcgctgc cggatggcgc acatattttg
      901 attaatgact tccgtattgg cagtcaggtt caggacgata tttaccagct ttggttgcaa
      961 agccagcgcc gattactgcc gatgctcatt catcgtgatg aagcgatggc tgaatgcctg
     1021 gcggctaagc aaccagtagg tgaatatcgc agtgatgcgc tggcggctga agagatactg
     1081 acgctggcga actggtgcct gttgaactac tccgggctga aaacgccagt cgggagtgca
     1141 tcatgagtat cctgacccgg tggttgctta tcccgccggt caacgcgcgg cttatcgggc
     1201 gttatcgcga ttatcgtcgt cacggtgcgt cggctttcag cgcgacgctc ggctgtttct
     1261 ggatgatcct ggcctggatt tttattccgc tggagcaccc gcgctagcag cgtattcgcg
     1321 cagaacataa aaacctgtat ccgcatatca acgcctcgcg tccgcgtccg ctggacccgg
     1381 tccgttatct cattcaaaca tgctggttac tgatcggtac atcgcgcaaa gaaacgccga
     1441 aaccgcgcag gcgggcattt tcaggtctgc agaatattcg tggacgttac catcaataga
     1501 tgaacgagct gcctgagcgc gttagccata aaacacagca tcttgatgag aaaaaagagc
     1561 tcggtcattt gagtgccggg gcgcggcggt tgatcctcgg tatcatcgtc accttctcgc
     1621 tgattctggc gttaatctgc gttactcaac cgtttaaccc gctggcgcag tttatcttcc
     1681 tgatgctgct gtggggtgta gcgctgatcg tacggcggat gccggggcgc ttctcggcgc
     1741 taatgttgat tgtgctgtcg ctgaccgttt cttgccgtta tatctggtgg cgttacacct
     1801 ctacgctgaa ctgggacgat ccggtcagcc tggtgtgcgg gcttattctg ctcttcgctg
     1861 aaacgtacgc gtggattgtg ctggtgctcg gctacttcca ggtagtatgg ccgctgaatc
     1921 gtcagccggt gccattgccg aaggatatgt cgctgtggcc gtcagtggat atctttgtcc
     1981 cgacttacaa cgaaaatctc aatgtggtga aaaataccat ttacgcctcg ctgggtatcg
     2041 actggccgaa agacaaactg aacatctgga tcctcgatga cggcggcagg gaagagtttc
     2101 gccagtttgc gcaaaacgtg ggggtgaaat atatcgcccg caccactcat gaacatgcga
     2161 aagcgggcaa catcaacaat gcgctgaaat atgccaaagg cgagttcgtg tcgattttcg
     2221 actgcgacca cgtaccaacg cgatcgttcc tgcaaatgac catgggctgg ttcctgaaag
     2281 agaaacagct ggcgatgatg cagactccac accacttctt ctcgccggac ccgtttgaac
     2341 gcaacctggg acgttttcgt aaaacgccga acgaaggcac gctgttctat ggtctggtgc
     2401 aggatggcaa cgatatgtgg gacgccactt tcttctgcgg ttcctatgcg gtgattcgtc
     2461 gtaagccgct ggatgaaatt ggcggcattg ctgtcgaaac cgtgactgaa gatgcgcata
     2521 cttctctgcg tttgcaccgt cgtggctata cctccgcgta tatgcgtatt ccgcaggcgg
     2581 cggggctggc gaccgaaagt ctgtcggcgc atatcggtca gcgtattcgc tgggcgcgcg
     2641 ggatggtaca aatcttccgt ctcgataacc cgctcaccgg taaaggactg aagtttgctc
     2701 agcggctgtg ctacgtcaac gccatgttcc acttcttgtc gggcattcca cggctgatct
     2761 tcctgactgc gccgctggcg ttcctgctgc ttcatgccta catcatctat gcgccagcgt
     2821 tgatgatcgc cctattcgtg ctgccgcata tgatccatgc cagcctgacc aactcgaaga
     2881 tccagggcaa atatcgccac tctttttgga gtgaaatcta cgaaacggtg ctggcgtggt
     2941 atatcgcacc accgacgctg gtggcgctga ttaacccgca caaaggcaaa tttaacgtca
     3001 ccgccaaagg tggactggtg gaagaagagt acgtcgactg ggtgatctcg cggccctaca
     3061 tcttccttgt tctactcaac ctggtgggcg ttgcggtagg catctggcgc tacttctatg
     3121 gcccgccaac cgagatgctc accgtggtcg tcagtatggt gtgggtgttc tacaacctga
     3181 ttgttcttgg cggcgcagtt gcggtatcgg tagaaagcaa acaggtacgc cgatcgcacc
     3241 gcgtggagat gacgatgccc gcggcaattg cccgcgaaga tggtcacctc ttctcgtgta
     3301 ccgttcagga tttctccgac ggtggtttgg ggatcaagat caacggtcag gcgcagattc
     3361 tggaagggca gaaagtgaat ctgttgctta aacgcggtca gcaggaatac gtcttcccga
     3421 cccaggtggc gcgcgtgatg ggtaatgaag ttgggctgaa attaatgccg ctcaccaccc
     3481 agcaacatat cgattttgtg cagtgtacgt ttgcccgtgc ggatacatgg gcgctctggc
     3541 aggacagcta cccggaagat aagccgctgg aaagtctgct ggatattctg aagctcggct
     3601 tccgtggcta ccgccatctg gcggagtttg cgccttcttc ggtgaagggc atattccgtg
     3661 tgctgacttc tctggtttcc tgggttgtat cgtttattcc gcgccacccg gagcggagcg
     3721 aaacggcaca accatcggat caggctttgg ctcaacaatg atgataacgc gatgaaaaga
     3781 aaactattct ggatttgtgc agtggctatg aggatgagtg cgttcccctc tttcatgacg
     3841 caggcgacgc cagcaacgca accactgatc aatgctgagc cagctgtagc cgcccagacg
     3901 gaacaaaatc cgcaggtggg gcaagtgatg ccgggcgtgc agggcgctga tgcgccagtc
     3961 gtggcgcaga acggtccttc gcgtgatgtg aagctgacct ttgcgcaaat tgcgccgccg
     4021 ccgggcagca tggtgctacg tggcattaac ccgaacggca gcattgagtt tggtatgcgc
     4081 agcgatgaag tggtgacgaa ggcgatgctc aacctcgaat acacgccatc gccatcgtta
     4141 ctgcctgtcc agtcgcagtt aaaggtttat ctcaatgatg agctgatggg cggctgccag
     4201 tgaccaaaga acagttgggt aaaaaaacgc tggcgcaaat gcccattaac ccactgttta
     4261 ttaccgactt caaccgtgtg cggctggagt ttgtcggcca ttatcaggac gtgtgcgaaa
     4321 acccggccag caccacgctt tggctggatg ttgggcggag cagtggactg gatctgacct
     4381 atcagaccct gaatgtgaag agtgacctgt cacacttccc ggtgccattc tttgacccgc
     4441 gcgataaccg tactaacacc ttgccgatgg tctttgcggg tgcgccggat gttgggctgc
     4501 aacaagcatc tgccattgtc gcctcgtggt ttggttcgcg ttctggctgg cgtgggcaga
     4561 acttcccggt gctctataac caattgccgg atcgcaatgc cattgtcttt gcaaccaacg
     4621 acaaacggcc ggacttcctg cgcgatcatc cggcggtaaa agccccggtg attgagatga
     4681 ttaaccatcc gcagaatcct tacgtcaaac tgctggtggt gtttggtcgt gacgacaaag
     4741 acctgttgca ggcagcgaaa ggtatcgctc agggtaacat tctgttccgt ggtgaaagcg
     4801 tggtagtgaa tgaagtgaaa ccgctgctac cgcgtaagcc gtacgatgcg ccgaactggg
     4861 tacgtaccga tcgtccggtc acatttggcg aactgaaaac ctatgaagaa cagttacaat
     4921 ccagcggtct tgagccagca gcgattaacg tttcgctaaa cctgccgccg gatctctacc
     4981 tgatgcgcag taccggcatt gatatggata ttaattaccg ctacaccatg ccgccggtga
     5041 aagacagttc gcggatggat atcagcctga ataaccagtt cctgcaatcc ttcaacctga
     5101 gcagtaaaca ggaggcgaac cgcctgctgc tgcggattcc ggtattacaa ggtttgctgg
     5161 atggcaaaac ggatgtctct attccggcgc tgaaactggg cgcgaccaac cagctgcgct
     5221 tcgactttga gtatatgaac ccgatgccgg gcggttcggt ggataactgt attaccttcc
     5281 agccggtgca gaatcatgtg gtgattggtg acgattccac catcgacttc tcgaagtatt
     5341 accacttcat cccgatgccg gatctacgcg cctttgctaa cgcgggcttc ccgttcagcc
     5401 ggatggcgga tctgtcgcaa accatcaccg tgatgccgaa aacgcctaac gaagcacaga
     5461 tggaaacgtt gctgaatact gttggtttta tcggcgcaca gacgggcttc ccggcgatta
     5521 atctgacggt gaccgatgat ggcagcacca ttcagggcaa agatgccgac atcatgatca
     5581 tcggtggtat cccggacaaa ttaaaagacg ataagcagat cgacctgttg gtgcaggcga
     5641 ccgaaagctg ggtgaaaaca ccgatgcgcc agaccccgtt ccccggcatt gtgccggacg
     5701 agagcgatcg tgcggcagaa acccagtcaa cgctgacctc ttccggtgcg atggcggcgg
     5761 tgattggctt ccagtcgccg tataacgacc agcgcagcgt gattgcgctg ctggcagata
     5821 gcccacgcgg ttatgaaatg cttaacgatg cggtgaacga tagcggcaaa cgcgccacca
     5881 tgttcggttc ggtcgcggtg atccgcgagt ccggtatcaa cagcctacgt gttggcgacg
     5941 tttattacgt aggtcatctg ccgtggttcg agcgcttgtg gtatgcgctg gcaaaccatc
     6001 cgattctgct ggcggtgctg gcggcaatca gtgtgatatt gctggcatgg gtactgtggc
     6061 gtctgctgcg aattattagt cgtcgtcgtc ttaacccgga taacgagtaa ttgaagatga
     6121 atgtgttgcg tagtggaatc gtgacgatgc tgctgttggc tgcctttagt gttcaggcag
     6181 cctgtacctg gcctgcctgg gagcagttta aaaaggatta catcagtcag gaagggcgcg
     6241 tcatcgcccc cagcgacgcg cgcaaaatca ccacctccga agggcaaagt tacggtatgt
     6301 tctttgccct ggcggctaac gaccgtgtag ctttcgataa tattctcgac tggacgcaga
     6361 acaatctcgc tcagggctct ttaaaagaac gtttgcccgc ctggctgtgg ggcaagaaag
     6421 agaacagtaa gtgggaagtg ctggacagca attcggcctc cgatggtgat gtctggatgg
     6481 cctggtcgtt gctggaggcg gggcgtttgt ggaaagagca gcgttatacc gacatcggca
     6541 gcgcgttgct aaaacgtatc gcgcgggagg aagtggtgac ggtgcctggg ctgggttcca
     6601 tgttgttacc gggcaaagtg ggttttgctg aggataacag ctggcgtttt aaccccagct
     6661 acctgccgcc gacgctggcg cagtatttca cccgctttgg cgcgccgtgg accacgctgc
     6721 gcgaaaccaa tcaacgttta ttgctggaaa ccgccccgaa aggtttttcg ccagactggg
     6781 tgcgctatga gaaagacaaa ggctggcagc taaaagccga aaaaacattg atcagcagct
     6841 acgacgctat ccgcgtttac atgtgggtag gcatgatgcc tgacagcgat ccgcaaaaag
     6901 cgcggatgct caaccggttt aaaccgatgg cgacattcac tgagaaaaac ggttatccgc
     6961 cggaaaaagt ggatgtggct acggggaaag cgcagggtaa aggaccgatc ggtttttctg
     7021 ccgccatgct gcccttttta caaaaccgcg atgcgcaggc cgttcagcgc cagcgcgtgg
     7081 ccgataactt tcccggcagc gatgcctatt acaactatgt gctgaccctg tttggacaag
     7141 gctgggatca acaccgtttc cgcttctcga caaaaggtga gttattacct gactggggcc
     7201 aggaatgcgc aaattcacac taaacatatt cacgctttcc ctcggtctgg ccgtcatgcc
     7261 gatggtcgag gcagcaccaa ccgctcagca acagttgctg gagcaagttc ggttaggcga
     7321 agcgacccat cgtgaagatc tggtgcaaca gtcgttatat cggctggaac ttattgatcc
     7381 gaataacccg gacgtcgttg ccgcccgttt ccgttctttg ttacgtcagg gcgatattga
     7441 tggcgcgcaa aaacagctcg atcggctgtc gcagttagcg ccgagttcaa atgcgtataa
     7501 atcgtcgcgg actacgatgc tactttccac gccggatggt cgtcaggcac tgcaacaggc
     7561 acgattgcag gcgacgaccg gtcatgcaga agaagctgtg gcgagttaca acaaactatt
     7621 caacggtgcg ccgccggaag gtgacattgc tgtcgagtac tggagtacgg tggcgaaaat
     7681 tccggctcgc cgtggcgaag cgattaatca gttaaaacgc atcaatgcgg atgcaccggg
     7741 caatacgggc ctgcaaaaca atctggcgct attgctgttt agtagcgatc gccgtgacga
     7801 aggttttgcc gtcctggaac agatggcaaa atcgaacgcc gggcgcgaag gggcctctaa
     7861 aatctggtac gggcagatta aagacatgcc cgtcagtgat gccagtgtgt cggcgctgaa
     7921 aaaatatctc tcgatcttta gtgatggcga tagcgtggcg gctgcgcaat cgcaactggc
     7981 agaacagcaa aaacagctgg ccgatcctgc tttccgcgct cgtgcgcaag gtttagcggc
     8041 ggtggactct ggtatggcgg gtaaagccat tcccgaacta caacaggcgg tgcgggcgaa
     8101 cccgaaagac agtgaagctc tgggggcgct gggccaggcg tattctcaga aaggcgatcg
     8161 cgccaatgca gtggcgaatc tggaaaaagc cctcgcactg gacccgcaca gcagcaacaa
     8221 cgacaaatgg aacagtctgc tgaaagtaaa ccgctactgg ctggcgatcc agcagggcga
     8281 tgctgcgctg aaagccaata atcctgaccg ggcagaacgc ctgttccagc aggcgcgtaa
     8341 tgtcgataac accgacagtt atgcagtgct ggggctgggc gatgtggcga tggcgcgaaa
     8401 agattatccc gccgccgaac gttattatca gcagaccttg cgtatggaca gcggcaacac
     8461 taacgccgtg cgcgggctgg caaatattta ccgccagcaa tcgccagaaa aagctgaagc
     8521 gtttatcgcc tcgctctctg ccagtcagcg gcgtagcatt gatgatatcg aacgcagcct
     8581 gcaaaacgac cgtctggcac agcaggcaga ggcactggaa aaccagggca aatgggcgca
     8641 ggcggcagca cttcagcggc aacgactggc gctggacccc ggcagcgtat ggattactta
     8701 ccgactttcg caggatctct ggcaggccgg acaacgcagc caggccgata cgttaatgcg
     8761 caatctggcg cagcagaagt cgaacgaccc agagcaggtt tacgcttacg ggctgtacct
     8821 acgcttacgg gctgtacctc tctggtcatg accaggacag agcggcgctg gcgcatatca
     8881 acagcctgcc gcgtgcgcag tggaacagca atattcagga gctggttaat cgactgcaaa
     8941 gcgatcaggt gctggaaacc gctaaccgcc tgcgagaaag cggcaaagag gcagaagcgg
     9001 aagcgatgct gcgccagcaa ccaccttcca cgcgtattga cctcacgctg gctgactggg
     9061 cgcaacaacg acgtgattac accgccgccc gcgctgcata tcagaatgtc ctgacgcggg
     9121 agccagctaa cgccgacgcc attcttggtc tgacggaagt ggatattgct gccggtgaca
     9181 aagcggcggc acgtagccag ctggcgaaac tgcccgctac cgataacgcc tcgctgaaca
     9241 cacagcggcg cgtggcgctg gcacaggcgc agcttggcga taccgcagca gcgcagcgga
     9301 cgtttaataa gttgatcccg caggcaaaat ctcagccacc gtcgatggaa agcgcgatgg
     9361 tgctgcgtga tggtgcgaag tttgaagcgc aggcgggcga tccaacgcag gcgctggaaa
     9421 cctacaaaga cgccatggtc gcatccggtg tgactacgac gcgtccgcag gataacgaca
     9481 cctttacccg actgacccgt aacgacgaga aagatgactg gctgaaacgt ggcgtgcgca
     9541 gcgatgcggc ggacctctat cgccagcagg atcttaacgt cacccttgag cacgattact
     9601 ggggttcgag cggcaccggt ggttactccg atctgaaagc gcacactacc atgttgcagg
     9661 tggatgcgcc gtattctgac gggcggatgt tctttcgcag tgatttcgtc aatatgaacg
     9721 tcggcagttt ctccactaat gccgatggca aatgggatga caactggggc acctgtacat
     9781 tacaggactg tagcggcaac cgcagccagt cggattccgg tgccagcgtg gcggtcggct
     9841 ggcgaaatga cgtctggagc tgggatatcg gtaccacgcc gatgggcttc aacgtggtgg
     9901 atgtggtcgg cggcatcagt tacagcgatg atatcgggcc gctgggttac accgttaacg
     9961 cccaccgtcg gcccatctcc agttctttgc tggcctttgg tgggcaaaaa gactccccga
    10021 gcaataccgg gaaaaaatgg ggtggcgtac gtgccgacgg tgtggggcta agtctgagct
    10081 acgataaagg tgaagcaaac ggcgtctggg catcgcttag tggcgaccag ttaaccggta
    10141 aaaatgtcga agataactgg cgcgtgcgct ggatgacggg ctattactat aaggtcatta
    10201 accagaacaa tcgccgcgtc acaatcggcc tgaacaacat gatctggcat tacgacaaag
    10261 atctgagtgg ctactcactc ggtcagggcg gttactacag tccgcaggaa tacctgtcgt
    10321 ttgccatacc ggtgatgtgg cgggagcgca cggaaaactg gtcgtgggag ctgggggcgt
    10381 ctggctcgtg gtcgcattca cgcaccaaaa ccatgccgcg ttatccgctg atgaatctga
    10441 tcccgaccga ctggcaggaa gaagctgcgc ggcaatccaa cgatggcggc agcagtcagg
    10501 gcttcggcta cacggcgcgg gcattacttg aacgacgtgt tacttccaac tggtttgttg
    10561 gcacggcaat tgatatccag caggcgaaag attacgcacc cagccatttc ctgctctacg
    10621 tacgttattc cgccgccgga tggcagggtg acatggattt accgccgcag ccgctgatac
    10681 cttacgccga ctggtaagtt ttcagatagc gcctctctta atgccgctgc gatcgggtat
    10741 actcgggcgg caatctggga tttccggggg gagacaattt gcgcgtaagt cgctcgttaa
    10801 caatcaagca gatggcaatg gtggcagccg ttgtcctggt gttcgttttt attttttgca
    10861 ccgttttgct gttccatctg gtccagcaga atcgctataa cacggctacg caactggaaa
    10921 gcattgctcg ctctgtccgc gaacccttat cttcagctat tttgaaaggc gatattcccg
    10981 aagcggaagc tattcttgcc agcattaaac cggcaggcgt ggtcagccgt gccgatgtag
    11041 tgctgcctaa ccagttccag gcgctgcgta aaagttttat tccagagcgc ccggtgccga
    11101 taatggttac tcgcctgttt gagctaccgg ttcaaatctc gctgggcgtt tactcgctcg
    11161 aacgtccggc aaacccgcag ccaattgcct atctggtact acaggcggat tccttccgta
    11221 tgtataagtt cgtgatgagc accctctcaa cgttagtgac catttactta cttttgtcgc
    11281 ttatcctgac cgtcgccatc agctggtgca ttaaccgcct gattttgcat ccgttacgca
    11341 atattgctcg cgaacttaac gccatcccag cccaggagct tgttggtcac caactggcat
    11401 taccgcgtct gcatcaggac gatgaaatcg gtatgttggt gcgcagttac aacctcaacc
    11461 agcaattgct gcagcgccat tatgaagaac agaacgaaaa tgcgatgcgc ttcccggtgt
    11521 cggatttgcc gaacaaagcc ttgctgatgg agatgctgga gcaggttgtc gcgcgtaaac
    11581 aaaccaccgc gctgatgatc atcacctgtg aaaccctgcg tgatactgcg ggcgtgctga
    11641 aagaggcgca acgagaaatt ctgctgctga cgctggtgga aaaactcaaa tcggtactgt
    11701 cgccacgtat gatcctcgcg cagattagcg gttatgactt tgctgtcatt gccaacggtg
    11761 tacaggaacc gtggcacgca atcaccttag gtcagcaagt gctcaccatc atgagcgagc
    11821 gcctgccgat tgaacgtatt caactccgtc cgcactgtag cattggcgtg gcgatgttct
    11881 acggcgatct caccgccgaa cagctttaca gtcgcgctat ttctgcggca tttaccgctc
    11941 gccataaagg caagaatcag attcagttct ttgatccgca gcagatggaa gccgcccaga
    12001 agcggttgac ggaagagagc gatatcctta atgcactgga aaatcatcag tttgctattt
    12061 ggttacagcc acaggtcgag atgaccagcg gtaaactggt cagtgcggaa gtgttactgc
    12121 gtatccagca accggatggc agttgggacc tgccggatgg cttaatcgat cgcattgagt
    12181 gctgtgggct gatggttacc gtcggtcact gggtgctgga agagtcctgt cgattgcttg
    12241 cagcctggca agagcgcggc attatgctgc ccttgtcggt aaacctctct gcgctgcaac
    12301 tgatgcaccc aaatatggtg gcggatatgc tggaactgtt aacccgctat cgcattcagc
    12361 cgggaacact gattctggaa gtgacagaaa gccgacgtat tgacgaccct catgctgcgg
    12421 tggcaatcct ccgtccgctg cgcaatgccg gagttcgggt ggcgctggat gatttcggca
    12481 tgggctacgc agggctgcgt cagctgcagc atatgaaatc gttgccaatc gacgtactga
    12541 aaatcgacaa aatgtttgtt gaaggcttgc cggaagatag cagcatgatt gctgcaatta
    12601 tcatgctggc gcagagcctg aacttacaaa tgattgccga aggcgtggag actgaagcac
    12661 aacgcgactg gctggcaaaa gcgggcgttg gtattgccca gggcttcctt tttgctcacc
    12721 cactccctat tgaaatcttc gaagagagtt acctggaaga aaagtagcta ccccaaactg
    12781 attacaaaac tttaaaaagt gctggtttgt gcgagccagc tcaaactttt taaccttttt
    12841 gtttcaatta tgatccaggt acatttctgt gatgttgtct gggtgttatt ttaaggccgc
    12901 aggtacccca taaccttaca agacctgtgg ttttactaaa ggacacccta tgaaaacctc
    12961 tctgtttaaa agcctttact ttcaggtcct gacagcgata gccattggta ttctccttgg
    13021 ccatttctat cctgaaatag gcgagcaaat gaaaccgctt ggcgacggct tcgttaagct
    13081 cattaagatg atcatcgctc ctgtcatctt ttgtaccgtc gtaacgggca ttgcgggcat
    13141 ggaaagcatg aaggcggtcg gtcgtaccgg cgcagtcgca ctgctttact ttgaaattgt
    13201 cagtaccatc gcgctgatta ttggtcttat catcgttaac gtcgtgcagc ctggtgccgg
    13261 aatgaacgtc gatccggcaa cgcttgatgc gaaagcggta gcggtttacg ccgatcaggc
    13321 gaaagaccag ggcattgtcg ccttcattat ggatgtcatc ccggcgagcg tcattggcgc
    13381 atttgccagc ggtaacattc tgcaggtgct gctgtttgcc gtactgtttg gttttgcgct
    13441 ccaccgtctg ggcagcaaag gccaactgat ttttaacgtc atcgaaagtt tctcgcaggt
    13501 catcttcggc atcatcaata tgatcatgcg tctggcacct attggtgcgt tcggggcaat
    13561 ggcgtttacc atcggtaaat acggcgtcgg cacactggtg caactggggc agctgattat
    13621 ctgtttctat attacctgta tcctgtttgt ggtgctggta ttgggttcaa tcgctaaagc
    13681 gactggtttc agtatcttca aatttatccg ctacatccgt gaagaactgc tgattgtact
    13741 ggggacttca tcttccgagt cggcgctgcc gcgtatgctc gacaagatgg agaaactcgg
    13801 ctgccgcaaa tcggtggtgg ggctggtcat cccgacaggc tactcgttta accttgatgg
    13861 cacatcgata tacctgacaa tggcggcggt gtttatcgcc caggccacta acagtcagat
    13921 ggatatcgtc caccaaatca cgctgttaat cgtgttgctg ctttcttcta aaggggcggc
    13981 aggggtaacg ggtagtggct ttatcgtgct ggcggcgacg ctctctgcgg tgggccattt
    14041 gccggtagcg ggtctggcgc tgatcctcgg tatcgaccgc tttatgtcag aagctcgtgc
    14101 gctgactaac ctggtcggta acggcgtagc gaccattgtc gttgctaagt gggtgaaaga
    14161 actggaccac aaaaaactgg acgatgtgct gaataatcgt gcgccggatg gcaaaacgca
    14221 cgaattatcc tcttaatctc accacttatg cccgcactcc cttccccgag tgcgggtgtt
    14281 tgcgcataat tgacccctgt gcctgaactt accacctggg tgagttcagg tttatttcac
    14341 ttcttccgtg acattttcat gttcttgcgg tctaacacga agtgttttta acgtcattta
    14401 gactgactga caacgggcag ttttttatta ccaggatagt tgatcagggg ttcacatgca
    14461 gggcacaaaa attcgacttt tagcgggcgg tttgctgatg atggccactg ctggctatgt
    14521 gcaggcagat gcgctccagc ctgatccagc atggcaacag gggacgcttt ccaacggttt
    14581 acagtggcaa gtgctgacca ccccccagcg tcccagcgat cgtgttgaaa ttcgcctgct
    14641 ggttaatatc ggttcgctcg ccgaaagtac acaacagagc ggttacagtc acgccatccc
    14701 tcgtattgcg ctaacgcaaa gcggtggcct tgacgcagca caggcgcgtt cattgtggca
    14761 gcaggggatc gaccctaaac gcccgatgcc gccggtaatt gtctcttatg acaccacgct
    14821 gtttaatctg agtttgccca ataaccgtaa cgatttgctg aaagaagcgc tctcttatct
    14881 ggcaaatgcc actggcaaat tgaccatcac accagaaacc atcaaccacg cgctgcaaag
    14941 tcaggacatg gtggcaacct ggcctgccga tactaaagag ggctggtggc gctatcgtct
    15001 gaaagggtca accttgttag gtcacgatcc tgccgatccg ctgaaacaac ccgttgaagc
    15061 ggaaaaaatt aaagatttct atcagaaatg gtacaccccg gatgcaatga cgctactggt
    15121 ggtgggaaac gtggatgcgc gctcggttgt cgaccaaatc aacaaaacgt ttggcgaact
    15181 gaaaggcaaa cgtgaaacgc cagctccggt gccgacgctt tctccgctgc gtgcggaagc
    15241 ggtgagtatt atgactgacg cggtgcgtca ggaccggtta tctatcatgt gggatacgcc
    15301 gtggcagccg attcgtgaat cagccgcact gctgcgctac tggcgtgcgg acctggcccg
    15361 cgaggcgctg ttctggcatg ttcagcaagc gttaagcgcc agtaacagca aagacatcgg
    15421 tcttggattt gactgccgtg tgctgtatct gcgtgcgcag tgtgccatca acatcgaatc
    15481 accaaacgac aagctgaaca gcaaccttaa tctggtggcg cgtgaactgg cgaaggttcg
    15541 cgataaaggt ctgccggaag aagagttcaa tgcgttagtg gcgcaaaaga aactggagct
    15601 gcagaaactg tttgccgcct atgcgcgggc tgataccgat attctgatgg gtcagcggat
    15661 gcgttcgctg caaaatcagg tcgtcgatat cgcgccggag cagtatcaga aactgcggca
    15721 ggatttcctt aacagcctga cggtggagat gttaaatcag gatctgcgtc agcagttgtc
    15781 gaatgatatg gcgttaatac tgctgcagcc gaaaggcgag ccggaattta acatgaaagc
    15841 gttgcaggcg gcctgggatc aaatcatggc cccatcgact gctgctgccg ccacctctgt
    15901 cgccacggat gacgtacatc ctgaagtgac ggatattcca cctgcacagt aatgcctgat
    15961 ggtgatgagt tagcacaacc atcaggtatt tatggatgag ctggatagag gggtaacact
    16021 ttatccagcc ttttgcatat gctgcgttta cgctggcatc gcctcacgcg ggataatcgc
    16081 gccgcgatac tgaataacgg tacttgcggt caggtgcccg cgtttcgccg cgttttccgc
    16141 actgccgccc gtcaaacata ccgccagata accggcgctg aaagagtcgc cagctgcggt
    16201 ggtatcgatc actttttctt tcggcagttt caccgccgga acatccacta acccttcgcc
    16261 agcaatggac accaggcaag aatccgcccc gcgtttcacc accacttctt tcacgcccgc
    16321 gttatgggtg cgcgcaatga cgtcttccac cggctgttga ccccacagcg cgtcttcatc
    16381 gtccagcgtc aggaaggcga tatccgtgca ttccagcatt tgttggtaca cctgctgtgt
    16441 ctcttctttg ctggcccaca ggcgcggacg atagttattg tcgaaaatca cttttccgcc
    16501 gttggcgcgg cattcgcgca gcagggaaag cagcttttcg cggctggtcg ggcttaagat
    16561 cgccaggcta atcccgctca ggtagagata atcgaaattc gccagctctt cgcaaatcgc
    16621 cgcagactgc tcactctcca gccagaattt ggcggcggct tcgttccgcc agtagtagaa
    16681 cgtacgctcg ccggtgctgt cggtttcaat gtagtaaagg cccggcagac ggttttccat
    16741 ccgttgggtc agggaagtat caacgttctc gccgtgccag gcgtccagca tttgctggct
    16801 aaaactgtcc gttcccagcg ccgttacgta atgaacggtt aatgccgcag gatcgacctg
    16861 acgggcgata tagacggaag tgttcagggt atcgccgccg aaaccgcgct taacgtccgc
    16921 gcctttctcg gaaagctcaa tcatgcattc gccaatcacg gcaatctttt tggacatagt
    16981 cgtgaacctg atctgtaaaa aattagcgtt agtttgcgct gtggtggttt gctggtcaac
    17041 catattaaaa cagtgttcca ttattttttt gagccagaac gtattcctga aagattccgt
    17101 tgtggagtga ggaaatttac cccggccagg cggtaaagtt ctgcccttgc gcgccgataa
    17161 tctttgtcga gtccgggcag catcactttt aaacacagga catctttgat gataaggcag
    17221 gttatccagc gaataagcaa ccctgaagca agcatcgaga gcttgcagga acggcgtttt
    17281 tggttgcagt gtgagcgtgc ttacacctgg cagccgatct atcaaacatg cggacggtta
    17341 atggccgtgg agctattaac ggtggtcacg catcccttga acccttcgca acgcctgccg
    17401 ccggatcgct attttactga aatcaccgtc agccatcgga tggaggttgt gaaagagcag
    17461 attgatttgc tggcgcaaaa agccgacttc tttgtagagc acgacctgct ggcatcggtc
    17521 aatattgatg gccctacgct catcgccctg cgtcagcaac caaaaatcct gcgccaaatt
    17581 gagcgtcttc cctggctgcg tttcgaactg gtggaacaca tccgcctgcc gaaagattca
    17641 acctttgcct cgatgtgtga atttggcccg ctgtggctgg atgattttgg caccgggatg
    17701 gcaaatttct ctgcgctaag tgaagtgcgt tatgactaca tcaaaatcgc gcgagagctg
    17761 ttcgtgatgc tgcgtcagtc gccggaaggc cgcacactct tttctcagct tttacatcta
    17821 atgaatcgct attgtcgcgg ggtgattgtc gagggcgtag aaacaccgga agagtggcgt
    17881 gatgttcaga actcgcccgc attcgccgca caaggctggt ttctttcacg cccggcaccg
    17941 atggaaacgc tgaatacggc ggttctggcg ctataagctg cctcattttc cgctgggctg
    18001 gactatcttt aggactggca caggaagaat atgaggaagc gaacgatgag caaggcaggc
    18061 aaaataaccg ctgcgatttc aggggctttc ttgttgttga ttgtcgtggc gatcattttg
    18121 attgcaactt ttgactggaa tcgactcaaa ccgaccatca accagaaagt ctctgcggag
    18181 ttgaatcgtc cgttcgctat ccgtggcgat ctgggcgtgg tgtgggagcg gcaaaaacaa
    18241 gaaactggct ggcgcagctg ggtgccgtgg cctcatgtac atgcagaaga catcattctt
    18301 ggtaatccac cggatattcc cgaagtcacg atggtgcatt tgccgcgagt agaggcaacg
    18361 ctggcaccgc tggcgctgct gaccaaaacg gtctggctgc cgtggttcaa gctcgaaaag
    18421 cccgacgcgc gcctgattcg cctctccgaa aagaacaata actggacgtt taatcttgcc
    18481 aacgatgaca acaaagacgc gaatgcaaag ccgtcggcat ggtcgtttcg gctggataat
    18541 attcttttcg atcaagggcg gatcgccatt gatgacaaag taagcaaagc ggatctggaa
    18601 atttttgttg atccgttagg caagccgctg ccgttcagcg aagttactgg atcgaaaggt
    18661 aaagcggata aagaaaaggt gggcgattac gtttttggcc tgaaggcgca gggacgttat
    18721 aacggtgaac cgctcacggg tacgggaaaa ataggcggta tgctggcgct gcgtggcgaa
    18781 gggacgccgt ttccggtaca ggctgatttc cgttcaggta atacccgtgt tgcttttgat
    18841 ggcgtcgtga atgacccaat gaagatgggc ggtgtcgatt tacggcttaa attttctggc
    18901 gattcactgg gtgatctcta tgaactgacg ggcgttctgc tgcccgatac cccgccgttt
    18961 gaaacggatg gtcggctggt agcgaaaatc gacactgaaa aatcgtcggt ctttgattat
    19021 cgcggtttta atgggcgaat tggcgatagc gatatccacg gttctctggt ctacaccacc
    19081 ggaaagccac gaccaaaact ggaaggtgat gtcgagtcgc ggcaattgcg gctggcggac
    19141 ctgggaccgt tgattggcgt tgattccggg aaaggggcag aaaagtcgaa acggtctgaa
    19201 cagaagaagg gcgaaaaaag cgttcagcct gcgggcaaag tgctgcctta tgaccgcttc
    19261 gaaaccgata aatgggacgt tatggatgcc gatgttcgct tcaaagggcg gcgcattgag
    19321 catggcagta gcctgccgat tagcgatctt tctactcata tcatcctcaa aaatgctgac
    19381 ctgcgcctgc aaccgctgaa atttggcatg gcgggcggca gcattgcgac gaatattcat
    19441 ctggaaggcg ataaaaagcc gatgcagggg cgggcagata ttcaggctcg tcgactgaaa
    19501 ctgaaagaac tgatgcccga tgtggaactg atgcagaaga cgctggggga aatgaacggt
    19561 gacgcggaac tacgcggtag cggtaactcg gtggcggcgc ttttaggcaa cagtaacggc
    19621 aacctgaaac tgttgatgaa tgacgggctg gtgagccgca acctgatgga gattgttggg
    19681 ctgaatgtcg gcaactacat tgtcggtgcg atatttggtg acgatgaggt gcgggtgaac
    19741 tgcgcggcgg cgaatctgaa tattgccaac ggcgtggcgc gcccgcagat ttttgctttc
    19801 gatactgaga acgcgttgat taatgttacc ggcacggcaa gttttgcttc agaacagctg
    19861 gatttgacta ttgatccgga gagtaaaggg attcggatta tcacactgcg ttcgccgctg
    19921 tatgtgcgtg ggacgtttaa aaatccgcag gctggggtga aagccggacc gctgattgcc
    19981 cgtggtgctg ttgctgcggc actggcaacg ctggtaacac cgacggcggc gttactggca
    20041 ctgatctcac cttccgaagg ggaggctaat cagtgtcgga cgatattgtc gcagatgaag
    20101 aagtgatgat aattgccgga tgtctgacat ccggcaatta aacagattta gatttttggc
    20161 aaagtgtgct ttgttcatgc cggatgcggc gtgaacgcct tatccggcct acaaatcctt
    20221 gtgaattcaa tatattgtat ggaaattcag tcctgataag cgtagcgcat caggcttttc
    20281 acacttatta caacgactga tgtcgcgtct catgggtcag cagcagggcg attaacgtca
    20341 agccagccat cgccgccaga tataacccca ccgcacctaa cccgtagtta gtctgcaacc
    20401 aggctgcgat atacggtgca acggaagccc cgagaatcga cgctacgttg taagagaatg
    20461 atgctccggt gtaacgcact tctgttggga acagctctgg taacagcgcg cccatcggcc
    20521 cgaaggtcag ccccatcaga cttaacccca gcagcaggaa ggcaaaaacc agaatcgggt
    20581 tgccagaacc gagcagcggg ttaaaggcga acagcgcgaa caggatgatc agcgtggtga
    20641 taattaccat gcttttacgg cgaccaaagg catcagccag taatccggcg actggcacca
    20701 tcacgccaaa accaataact gccatcatca gcatccacaa cacttcgtta cgcggcaggc
    20761 caagcccaac tggcgcggcg gcggtactga aggtcataga gtagaccgtc atgatgtaaa
    20821 acagcgtata ggttgccagc atgatgaacg tacccagtac ggttacgcga acatgtttgg
    20881 tcagcagcgt acccagcggg atcttcacct gttttttcgc tttagcgact ttctcaaaca
    20941 ccggcgactc atgcagcgac acgcgaacat acaggccgat aatgaccagt accgccgaga
    21001 agataaatgg cacacgccag ccccagctca taaactgctc gtcggtcagt agccaggaaa
    21061 gcagcaagaa agtgccgttg gcaaagaaga agccaatcgg tgcgcccagc tgtgggaagg
    21121 agccatacag tgcacgtttg cgcggtgggg cgttttcagt cgccagcagc gccgcgccgc
    21181 cccattcacc gcctaagccc agaccctgac caaatcgagc cagcgccagc agcagcgggg
    21241 cgaaaatacc aatcgtggca tagcccggca gcagaccaat caccacggtt gaaatcccca
    21301 tcgttagcaa cgaggcgacc agcgtcgctt tacgcccaac gcgatcgcca aaatgaccaa
    21361 aaacggcaga gccaatgggg cgcgcgacga aggcgatggc gaaggtggcg agcgactgta
    21421 gcgttgctgc cgttggatcg ccctgcggga agaagatatg cggaaacaca ataacggccg
    21481 cagtggcgta aatgtaaaag tcgaagaact caatggccgt gccaatgaga gaggcgacaa
    21541 ggactttatt acgcgagttg atcggcgtgt attcttgctc gtggtcgagt gttgtggctg
    21601 ttgcttgcat aatcttttct tattttttgg ctaacgaata gccatattac gcacagcaaa
    21661 agtgatattt caatgtgtaa caaatcgcgg aaatggtcaa aaaccaggca aaaaggggat
    21721 aatgtttagg ccagtgattt ttcttctatg aaatgcttca cataatttta taaatagtgg
    21781 ataaaactgg tttcaggtta aataaaagtt accaactgga tttatatgct ggggttatga
    21841 gttggaatga aattgtttgc tcccaaatag atggctgaag aggctctggg gaagagtttc
    21901 aagaggttat gtgcgcatta tagccggatg cgatgctggc gcatcttatc cggcctacgg
    21961 ggagccaggc cgaataaaat cgtatgccgg ataaggcgtt tacgccgcat ccggcctatt
    22021 ttaaggctgc gttttccctg gcattcgcgg gtcgtcttta tattcggcgg tggcaatcca
    22081 cgccgcgcaa aacagcgtca aacgggcgaa gaagtagaaa aatgccatca gccccagcac
    22141 ggaaccaaat gctgcgccag agggggattt catcagcgat ggcagggtgt aggtcatcac
    22201 gattttaatc acttcaaaac caatagcagc cagaaatgtt ccgcgaatca gggctttttt
    22261 gcgcggacgg tggcgcggta accgccagaa gatccagaag aaaagcagat agttggcgaa
    22321 gatggaaatc gccaggccaa tcaaccgcca cgtcggtttc agccactcaa tgctgttcag
    22381 gtgcagggcg ctaataatca tttgctgcgc cgaaccggca accgaggtga tcgaaagcgt
    22441 cacaatcagc gcaatcaaca aaccaatcag cgaaataaaa tcacgtagat attttaccca
    22501 gaacttctcc tgatcttgcg gcgagcgttc ccagacatcg cgcgactggg cgcgaatcgc
    22561 ttcacgcagg ttacccatcc agttgatgcc ggaataaagc gccacagcca ggccgacaag
    22621 ccctacagtc gtacgctgct gaacggcggt gttgatggtg tttttcaacg tggcggctag
    22681 cgtcggatcg ctgatgtttt gcagaatttt gtcgaagata tcctgtagca acatcggatg
    22741 ggaggccagc acaaagcccc ctgcggcaaa cgacaccatc aaaatcggga tcatcgacaa
    22801 aaatgagaaa taggtgatag ccgcaccaaa ctggttaccc agccgatcgt taaagcgttc
    22861 tgtcgcgcga atcaggtgcg caataaccgg ttggcgttgg actttttcgg cagtggtggt
    22921 gacggtttcc agcgcctggc taactttgcc gcctttctcg ctattatcca actgcttaat
    22981 cggctcgtgc tccagatcct gggtgggacg tttgatctcg ttttcctgcg tcatccgctc
    23041 ttttgtcctt ctgttgtggt cattcttaaa aagtatagtc ggtcagtcca ggtactcttt
    23101 cattacgcca gccagccact gcataaacag gtttacacgc cgggaaagct cccgacgctg
    23161 cggataaacc agcgaaaggg agagcggctc ggcacggtag cctggcaaca cttcaataag
    23221 ctgcccggca cgcagggctt cgcgcacggc aatgcgcggg atctgaataa tccccagacc
    23281 ggcaagaccg gcggcgagat agttttcgct actgtttacc gtcaacatgc cgccagactt
    23341 aaaccactgg acgccattaa cgctggcaac ctcaaaacct aacggatgta cacccaggtg
    23401 cggtgtataa cgcaccattg cgtgtgaagt cagatcatcg gggctttgcg gataaccaaa
    23461 gcgcgtcaga tagtgcggac tggcacagtt gaccatggtc agtttgccga ggggacgggc
    23521 gataacgcca tcttccggta acgcgccagt gcgtatcacg caatcaaaac catcatgaag
    23581 aatatctacc ggacggttat ggctactcag ttccagctca attcccggat gctgatagag
    23641 aaattccgac agacgcggca gtaacaggtt tttcgcgatt cctgacggga tgtcgatgcg
    23701 taatttaccg ctgatactgg tggcatcctg ttgaaacaga ccgtccagtt cgctgagatt
    23761 actcaacaca tcttttgccc gttgataata ggtcatgcct tctggcgtga gtttgacccg
    23821 tcgcgtagtg cgctggagaa gctgggtgcc aagttgatgt tccagtgcct gtatctggcg
    23881 cgaaacactt ccctttggca aagcaaagaa atccgccgcg cgggaaaaac tttccagctc
    23941 cgcgactttg atgaacaact gcattgcgtg aattttatcc atttctttga ccgattgttg
    24001 ttcacagcga aacagtaacg cgcatatcgc attgtttatt gttttttcta acatctaatg
    24061 ccgggtttca caatgttgtc atgacgaatg tggcgatttc tcagcaggaa aagggcggaa
    24121 tagctttatg agaaaggtga atccccttaa acagagcgta tttttcagtg attcccttta
    24181 aagtgaatag aggacgtatt ggctatttgt gcttattttt gtctcattgc catttgttat
    24241 tttggcttgt agtcgcggaa tttcattttt aattgcatgc gtactaacca ccaatataag
    24301 gaaatcctca aaattgtttt aaatagtgcc ctcgacatgt aatttcaggc gtttagctgt
    24361 aactatttat atcggtaact attttctacg cgtcatctct gtagaatggc caccgccaaa
    24421 tggcgaattc ttattttttc tcgcgggtaa tacccttcca ctacttaagt ctttttaacg
    24481 tattcaggtg gagattcagt tatgcaaata gtcatgtttg acaggcagtc aatatttatt
    24541 catggaatga aaatcagttt acagcagcgt attccaggag tgagtattca gggggccagt
    24601 caggcagacg agttatggca aaagctggaa agttaccctg aagccttagt tatgctcgat
    24661 ggcgatcagg atggtgagtt ttgctactgg ttgctgcaaa aaaccgtggt gcagtttcct
    24721 gaggttaagg tgttaatcac ggcgacggat tgcaataaac ggtggttaca ggaagttatt
    24781 cattttaatg tgctggccat tgtgcctcgt gattcaaccg tcgagacatt tgcgttggcg
    24841 gtaaatagtg cggcgatggg gatgatgttt ctgcctggag actggcggac gacgccggaa
    24901 aaggacatta aagatctcaa atcgttaagc gcccgccagc gggagatttt aaccatgtta
    24961 gcggcgggcg aatcaaataa ggagatcggc agagcgctga atatcagtac cggaacggta
    25021 aaagcgcatc tggaatccct gtatcgccgt ctggaagtga aaaatcgcac ccaggcggca
    25081 atgatgttaa atatctcctc ctg
//
LOCUS       DAJTZL010000043        24621 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.043, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000043 DAJTZL010000000
VERSION     DAJTZL010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24621
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.043"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(131..1780)
                     /gene="actP"
                     /locus_tag="OO544_001571"
     CDS             complement(131..1780)
                     /gene="actP"
                     /locus_tag="OO544_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HCR6767194.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKLVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMMGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSITVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(1777..2090)
                     /locus_tag="OO544_001572"
                     /pseudo
     CDS             complement(1777..2090)
                     /locus_tag="OO544_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
     gene            complement(2290..4248)
                     /gene="acs"
                     /locus_tag="OO544_001573"
     CDS             complement(2290..4248)
                     /gene="acs"
                     /locus_tag="OO544_001573"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HCR6767195.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINVPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGKIDWQEGRDLWWHDLVEQASDQHQAEKMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            4642..6078
                     /gene="nrfA"
                     /locus_tag="OO544_001574"
     CDS             4642..6078
                     /gene="nrfA"
                     /locus_tag="OO544_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HCR6767196.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            6123..6689
                     /gene="nrfB"
                     /locus_tag="OO544_001575"
     CDS             6123..6689
                     /gene="nrfB"
                     /locus_tag="OO544_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HCR6767197.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASDDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            6686..7357
                     /gene="nrfC"
                     /locus_tag="OO544_001576"
     CDS             6686..7357
                     /gene="nrfC"
                     /locus_tag="OO544_001576"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HCR6767198.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            7354..8310
                     /gene="nrfD"
                     /locus_tag="OO544_001577"
     CDS             7354..8310
                     /gene="nrfD"
                     /locus_tag="OO544_001577"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HCR6767199.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            8330..10048
                     /locus_tag="OO544_001578"
     CDS             8330..10048
                     /locus_tag="OO544_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HCR6767200.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWEFKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDAASARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFCH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRAQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDE"
     gene            10041..10424
                     /gene="nrfF"
                     /locus_tag="OO544_001579"
     CDS             10041..10424
                     /gene="nrfF"
                     /locus_tag="OO544_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HCR6767201.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVSEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            10421..11017
                     /gene="nrfG"
                     /locus_tag="OO544_001580"
     CDS             10421..11017
                     /gene="nrfG"
                     /locus_tag="OO544_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HCR6767202.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTCAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            11359..12672
                     /gene="gltP"
                     /locus_tag="OO544_001581"
     CDS             11359..12672
                     /gene="gltP"
                     /locus_tag="OO544_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HCR6767203.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(12751..13440)
                     /locus_tag="OO544_001582"
     CDS             complement(12751..13440)
                     /locus_tag="OO544_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCR6767204.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(13534..15681)
                     /gene="fdhF"
                     /locus_tag="OO544_001583"
     CDS             complement(13534..15681)
                     /gene="fdhF"
                     /locus_tag="OO544_001583"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(15262..15264),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HCR6767205.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVTERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            complement(15879..17345)
                     /gene="mdtP"
                     /locus_tag="OO544_001584"
     CDS             complement(15879..17345)
                     /gene="mdtP"
                     /locus_tag="OO544_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HCR6767206.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            complement(17342..19393)
                     /gene="mdtO"
                     /locus_tag="OO544_001585"
     CDS             complement(17342..19393)
                     /gene="mdtO"
                     /locus_tag="OO544_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HCR6767207.1"
                     /translation="MSALNSLPLPMVRLLAFFHEELSERRPGRVPQIVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGRMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAISQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQHAVAEGQCWQSDWRISESEAVAARECNLENICQTLLQLGQMNPNTPPTPA
                     AKPPSMVADAFTNPDYIRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVSNPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLD
                     SEERALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGL
                     ATALSRSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGAT
                     Q"
     gene            complement(19393..20424)
                     /gene="mdtN"
                     /locus_tag="OO544_001586"
     CDS             complement(19393..20424)
                     /gene="mdtN"
                     /locus_tag="OO544_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HCR6767208.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSINATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            complement(20443..20718)
                     /locus_tag="OO544_001587"
     CDS             complement(20443..20718)
                     /locus_tag="OO544_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6767209.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILMLITRRVIQRANINLAFVGIIYTALFALYAMLFWLAFF"
     gene            complement(20927..22912)
                     /gene="yjcS"
                     /locus_tag="OO544_001588"
     CDS             complement(20927..22912)
                     /gene="yjcS"
                     /locus_tag="OO544_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HCR6767210.1"
                     /translation="MNNSRLFRLSRIVIALTAASGMMVNTANAKEEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKPYYRGEDYKFDINAPAPETV
                     NPSLWRQSQLNGISGLFKVTDRMYQVRGQDISNITFIEGDTGIIVIDPLVTPPSAKAA
                     LDLYFQNRPQKPIVAVIYTHSHTDHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSRRALYSYGLLLAHNPQGNIGNGLGVTLASGYPSIIAPNKTITKTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVWGNKHINDYIGKYRDTIKYIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVQALGGSARVINLAQEANKQGDYRWSAELLKQVIAANPGDQVAKNLQANNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTIGSPDTIRGMSVEMLFDFMSVRLDSAKA
                     AGKNISLNFNMSNGDNLNLTLNDSVLNYRKTLQSQADASFYISREDLHAVLTGQAKMA
                     DLVKAKKAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
     gene            23165..23482
                     /locus_tag="OO544_001589"
     CDS             23165..23482
                     /locus_tag="OO544_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HCR6767211.1"
                     /translation="MQPVFLFKKRTGAMGGHVAGTAGFCVAVPAFYSWSMSEVIDKDT
                     RSSERTDYPHYASEVALAFGSRVVGLELAKMIVDAWLGAQYEGGRHQQRVEAITAIEQ
                     RRN"
     gene            23551..23880
                     /gene="yjdP"
                     /locus_tag="OO544_001590"
     CDS             23551..23880
                     /gene="yjdP"
                     /locus_tag="OO544_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjdP"
                     /protein_id="HCR6767212.1"
                     /translation="MKRFPLFLLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNPDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(24027..>24621)
                     /gene="phnP"
                     /locus_tag="OO544_001591"
     CDS             complement(24027..>24621)
                     /gene="phnP"
                     /locus_tag="OO544_001591"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HCR6767213.1"
                     /translation="GLHDLTDRWSPGSFQQFLLTHYHMDHVQGLFPLRWGVGDPIPIY
                     GPPDEQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHS
                     RVAWLSDTAGLPEKTLKFLRNNQPQVMVMDCSHPPRADAPRNHCDLNTVLALNQVIRS
                     PRVILTHISHQFDAWLMENALPSGFEVGFDGMEIGVA"
BASE COUNT         5587 a         6449 c         6708 g         5877 t
ORIGIN      
        1 tatcattttc tacgactgtc aaaaatcgct cattttttaa tgagtttatt tgtttaatat
       61 tttgggaaaa ggtgatgcat gctggagagg aagagtattc cccggtcagg cgaccgggga
      121 aggggagaga ttaatgtgcg cggccttgct caacgccaaa gccggtctgg gagcggataa
      181 actgcgcgcg gaacagttca cgctcgcgcg ccccttccgc tgagttatcg gttgccgaga
      241 agaaccagat gccgaggaat gccacggtga tagagaacag cgccgggtat tcatacggga
      301 agatggcttt ttcgtgacca aggatctgta cccaaatcgt cgggccgagg atcatcagta
      361 ctactgcggt aatcagcccc agccagccac ccatcatcgc gccacgcgtg gtcagtttcg
      421 accagtacat agaaagcaga atgatcggga agttacagct cgccgcgatg gcaaacgcca
      481 gccccaccat aaaggcgatg ttctgattct caaacagcac gccgaggata atcgcaatca
      541 cgccgaggat cagtacggtg atttttgata cccgcagctc ttcacgttcg gtcgcgcctt
      601 ttttgaagac gttagcgtac aagtcatgcg aaaccgcgga tgcgcccgcc agcgtcagac
      661 ccgcaaccac cgcgaggata gtggcgaaag caaccgctga aataaaaccg aggaacaggt
      721 tgccgcccac tgcattcgcc aggtgaacgg ccgccatgtt gttaccacca atcagatggc
      781 ccgccgcgtc tttatattcc ggattcgcac caaccagcat gatcgcgccg aagccgataa
      841 taaaggtcag aatatagaag tagcccataa acccggtggc gtagaacacg ctcttacgtg
      901 cttcgcgggc atcgctgact gtaaagaagc gcatcagaat gtgcggcaag cccgccgtac
      961 caaacatcag tcccagaccc agagagagtg cggagatcgg atctttcacc agcccgcccg
     1021 gcttcatgat gtcgacacct ttcgggtgta ccgccatcgc ttcactgaac agattgttga
     1081 agctaaagcc gacgtgtttc atcaccataa aggccataaa gctggcaccg aacagcaaca
     1141 gcacggcttt gataatctgt acccaagtgg tcgccagcat gccgccgaac aggacgtaca
     1201 tcatcatcag cacgccgacc agcaccaccg caatgtgata gttaaggcca aacagcagct
     1261 cgatcagttt acctgcgccc accatctggg cgataaggta aagcgccacc accaccagag
     1321 aaccacaggc cgaaagaata cgaatcggcc cttgtttcag acggtaagag gccacatcgg
     1381 caaaggtgta gcgccccagg ttacgcagac gttcggcaat caggaacaaa atgatcggcc
     1441 agcccaccag gaagcccagc gagtaaatta agccgtcata gccggaggta aacaccagcg
     1501 cggagatccc caagaatgag gcggcggaca tatagtcccc ggcaatcgcc agcccgttct
     1561 ggaagccagt gatattgccg cctgcggtgt agtagtcgct acgagaacgt acgagttttg
     1621 atgcccagta ggtaatgccg agcgtaaaca cgacgaaaat caggaacata ataatcgcct
     1681 gccagttcgt tggctggcgc tctacggccc cgctaatagc atccgcggcg ttagctgcga
     1741 aagggagtgt ggcggcaagc gccgtcagaa ctcttttcat gatgcttgta cctcatgcag
     1801 gacttcatta ttaagacggt cgaattcgcc gttcgcccgc cagatgtaga taccggtgag
     1861 aacaaaggag atcacaatca ctccaacacc aattggaata ccgcgtgtga cgctggtgtt
     1921 cggattcagc ggcgtgccag ccagccgggc gcgaaggcga tcagtaaaat aaagccgata
     1981 taaactgcca gcataataat cgacaggatg gtggcaaacc gttgccgttt ttcgactaac
     2041 tccctgaaat gcgcattgtc ttctatccgc tgataaatag tgccattcat cacagattct
     2101 ccagaggtaa tgtagggatt gttttaattc ccgctccctt atgggagaag gttaacgctc
     2161 gggtaaccct tgccgaatgt aggccggata aggcgtttac gccgcatccg gcaatcaatg
     2221 cctgatgcga cgctgtcgcg tcttatcagg cctacaaacc gttaccgact cgcatccggc
     2281 aattgtgggt tacgatggca tcgcgatagc ctgcttctct tcaagcagct tctcgactac
     2341 gccaggatcg gcaagcgtcg aggtatcgcc caggttgctg gtatcgcccg ccgcaatttt
     2401 gcgcagaata cggcgcataa ttttgccgga gcgggtttta ggcagggagt cggtccagtg
     2461 cagcacgtct ggcgtcgcca gcgggccaat ctctttacgc acccagttgc ggacttctgc
     2521 gtacagttct ggtgacggtt cctccccgtg attaagcgtg acgtaggcgt agatcgcctg
     2581 acctttaata ttgtgcggaa tacctactac ggcggcttcg gcaatcttcg gatgcgccac
     2641 cagcgccgac tcaatctctg ccgtccccag acggtgaccg gagacgttca gcacgtcgtc
     2701 cacacgcccg gtgatccagt aatagccatc ttcatcgcga cgcgcgccgt cgccgctgaa
     2761 atacatattt ttgaaggtgg agaagtaggt ctgttcaaaa cgttcgtgat cgccaaacag
     2821 cgtacgcgcc tgacccggcc aggagtcggt gatcaccagg ctgccttcgg tagcgccttc
     2881 cagcgggtta ccttcgttat cgaccagcgc cggttgcacg ccgaagaacg gacgtgttgc
     2941 cgaaccggct ttcagctcgg tagcgccagg cagcggcgtg atcatgaaac cgccagtttc
     3001 ggtctgccac caggtatcga ccaccggaca tttctcgttg ccgatttttt tccagtacca
     3061 ctcccacgct tccgggttaa ttggctcgcc cacggaaccg agaatgcgca gcgacgaacg
     3121 gtcggtaccc tcgatcgctt tatcgccttc cgccatcagc gcgcggatcg ccgtgggtgc
     3181 ggtatagaga atattgacct gatgcttgtc caccacctgc gccatacggg caggcgtcgg
     3241 ccagttgggt acgccttcaa acatcagcgt ggtcgcaccg caggccagcg ggccgtacag
     3301 caagtaactg tgtccggtca cccagcccac atcggcggtg caccagtaga tatcacccgg
     3361 atgataatca aagacatatt taaaggtcag cgccgcgtac accagataac cgccggtagt
     3421 atgcagcaca ccttttggct taccggtaga accggaggtg tagagaataa acagcggatc
     3481 ttcggcgttc atcttttccg cctggtgctg atcgctcgct tgctcaacca ggtcgtgcca
     3541 ccacaggtcg cgcccttcct gccagtcaat tttcccgcca gtacgcttca gtaccaccac
     3601 atgctctacg ctggtgacgt tcgggttttt cagcgcgtca tcaacgtttt tcttcagcgg
     3661 aatactgcgc ccggcacgca caccttcgtc ggaagtgatc accagtcgtg agttggaatc
     3721 aataatgcgc ccggcaacgg cttccggcga gaagccgccg aaaatcaccg aatgcaccgc
     3781 gccaatgcgg gcgcaggcca gcatcgcaac cgcggcttcc ggcaccatcg gcatataaat
     3841 cgccaccaca tcaccttttt taatgcccag ctcgagcagg gtattggcga agcggcagac
     3901 gtcgcggtgc agctctttat agctgatatg tttgctctgg ctggcgtcgt cgccttccca
     3961 gatgatggcg gtacgatcgc cgttttcttg cagatggcgg tcaaggcagt ttgccgccag
     4021 attcagcgtg ccgtcctcgt accatttaat ggacacatta ccgggggcaa aggaggtgtt
     4081 tttcaccttc tggtaaggtt tgatccagtc aagaattttt ccctgttcgc cccagaaggt
     4141 atcaggtacg ttaatagatt gttgatacat cgcctcgtac tgctgagggt ttatcaggca
     4201 acggtctgcg atgttggcag gaatggtgtg tttgtgaatt tggctcatgc ttttgttctc
     4261 cttgtagggt gttaataata tgtggcataa gcgttaaatg taggggtatt ggcagttttg
     4321 tttagtattt gggcgacaga tcacgcaaaa gtagaattgt gcaaataaac ggcagggtaa
     4381 tttttgaagg tcagcaacaa aagttgatta attctttgag gaacatgcag ttatgcattg
     4441 ctgttgaaaa agaggaagat actaactaac tctaaagtgg tattttacat gcacttacaa
     4501 ttgattaaag acaacattca cagtgtggtt atttgttaca cataggggcg agcaatgtca
     4561 tgacagtgta ggtgcggtta ctgtcgtgaa aaacaataaa aaccgccatt gtaacaatgg
     4621 cgcaattcgg atgaagcccc tatgacaagg ataaaaataa acgcacgccg tatcttcagc
     4681 ttattgattc cttttttctt tttcacttct gttcacgctg aacaaacggc agctcccgca
     4741 aaacctgtaa ctgtggaagc gaagaatgaa acctttgccc cgcagcatcc cgatcaatat
     4801 ctctcctgga aagccacctc ggaacagtca gagcgtgttg acgccctggc ggaagatcca
     4861 cggctggtga tcctgtgggc ggggtatccc ttctcgcgcg attacaacaa gccgcgtgga
     4921 catgcctttg ctgtgaccga tgtgcgtgaa accctgcgta ccggtgcgcc gaaaaacgct
     4981 gaagatggtc cgctaccgat ggcgtgctgg agttgtaaaa gcccggatgt ggcgcgtctg
     5041 atccagaaag acggcgaaga tggctacttc cacggtaagt gggcgcgcgg cggcccggaa
     5101 atcgtcaaca acttaggttg tgccgactgc cataacaccg cctcaccaga gttcgccaaa
     5161 ggcaaaccgg agttaaccct ttcccgtccg tatgcggctc gcgcgatgga agccattggt
     5221 aaaccttttg agaaagccgg acgtttcgac cagcaatcga tggtttgcgg tcagtgccat
     5281 gtggagtatt acttcgacgg caaaaacaaa gcggttaaat tcccgtggga tgacggcatg
     5341 aaagtcgaaa atatggagca gtattacgac aaaattgcct tctctgactg gactaactcc
     5401 ctgtcgaaaa cgccaatgct gaaagcgcag cacccggaat atgaaacctg gacagcgggc
     5461 attcacggta aaaacaacgt gacctgtatc gactgccata tgccaaaagt gcagaacgcc
     5521 gaaggcaaac tctataccga ccataaaatt ggtaatccgt ttgataactt cgcccagact
     5581 tgtgcgaact gccataccca ggacaaagct gccttgcaaa aagtggtcgc ggaacgtaag
     5641 cagtcgatta acgacctgaa aatcaaggtt gaagatcaac tggttcacgc tcacttcgaa
     5701 gcgaaagcag cgctggatgc aggcgcgacg gaagctgaaa tgaagccaat tcaggacgat
     5761 atccgtcatg cccagtggcg ctgggatctg gcgatcgctt cccacggcat tcatatgcac
     5821 gcaccggaag aaggtttacg gatgctcggt acggcgatgg ataaagcggc ggatgcacgc
     5881 accaaactgg cgcgcctgct ggcgaccaaa ggcatcaccc atgaaatcca gatcccggat
     5941 atctcaacca aagagaaagc ccagcaggcc attggcctga acatggaaca aatcaaggcc
     6001 gagaagcagg acttcatcaa aacggtgatc ccgcagtggg aagaacaggc acgtaaaaac
     6061 ggtctgttaa gccaataacc ccgttccgcc tcgcaagggg cggaaaacac aatggagtga
     6121 atatgagcgt attacgttcg ttattaactg ccggggtgct ggcgtcgggc ctgttgtgga
     6181 gcctgaacgg aattaccgct acccctgcgg cgcaggcatc tgatgatcgc tacgaagtta
     6241 cccagcagcg taacccggat gccgcctgtc tggactgtca taaaccagat accgaaggta
     6301 tgcatggcaa acatgcttcc gttatcaacc cgaataacaa actgccggtc acctgcacca
     6361 actgtcacgg ccagccatca ccgcaacacc gcgaaggggt gaaagatgtg atgcgcttta
     6421 acgagccgat gtacaaggtt ggagagcaga acagcgtctg tatgtcctgt catctgccgg
     6481 aacagttgca aaaagcgttc tggccgcacg atgtccacgt aaccaaagtg gcgtgcgcca
     6541 gctgccattc cctgcatcca cagcaagata cgatgcagac gttaagtgac aaaggacgga
     6601 ttaagatttg cgtcgattgc cacagcgatc agcgcaccaa tccgaacttt aacccggcgt
     6661 ctgttccgtt gcttaaggag cagccatgac ctggtctcgt cgccagtttc tcaccggcgt
     6721 cggcgtgctg gcagccgtca gcggcaccgc agggcgcgtg gtggcgaaaa cgttgaatat
     6781 caatggggtg cgttacggca tggtgcatga cgagtcgtta tgcatcggct gtaccgcctg
     6841 tatggatgct tgtcgggaag tgaacaaggt gccggaaggc gtctcgcgcc tgacgattat
     6901 tcgtagcgag ccgcagggcg aatttcctga tgtgaaatat cgcttcttcc gtaagtcttg
     6961 ccagcactgc gatcatgcgc cgtgcgttga cgtctgcccg accggtgcgt cttttcgtga
     7021 tgccgctagc gggattgtcg atgttaatcc tgatctctgc gtcggttgtc agtactgcat
     7081 cgccgcctgt ccgtaccgcg tgcgctttat ccatccggtc acgaagacgg cggacaaatg
     7141 cgatttctgc cgtaaaacca atttgcaggc cggtaagctg cccgcgtgtg ttgaggcttg
     7201 cccgacgaag gcgctgacgt ttggcaatct ggacgatcct aacagtgaga tttcgcaact
     7261 gctgcgccag aagcctactt accgctacaa gctggcgctg ggaaccaaac cgaagctgta
     7321 ccgcgtaccg tttaaatacg gggaggtgag ccaatgacgc agacttccgc atttcatttt
     7381 gaatcgctgg tgtgggactg gccgattgcc atctacctgt ttttgattgg tatttctgcc
     7441 ggtctggtga cgctggccgt gctgttacgt cgcttctacc cgcaggcggg cggtgcagac
     7501 agtacgttgc tgcgcaccac gctgattgtc gggccgggcg cggtgatcct cggtctgttg
     7561 atcctcgtct tccacctgac aagaccgtgg accttctgga agctgatgtt ccactacagt
     7621 tttacctcgg tgatgtcgat gggggtgatg ctgtttcagc tctacatggt ggtgctggtg
     7681 ctgtggctgg cgaaaatctt tgaacatgat ttgcttgccc tgcaacaacg ctggttgccg
     7741 aagctgggga tcgtgcaaaa ggttctgagc ctgctgacgc ccgttcatcg cggactggaa
     7801 acgctgatgc tggtgttggc ggtgctgctg ggggcttata ccggctttct gctgtcggcg
     7861 ctgaaatcgt atccgttcct caataacccg atcctgccgg tgctgttcct cttctccggc
     7921 atctcgtccg gtgcggcggt ggcgctgatc gccatggcga tacgccaacg cagtaacccg
     7981 cattccacgg aagcgcactt tgttcaccgg atggaaatcc ccgtggtatg gggtgaaatc
     8041 ttcctgctgg tggcgttttt tgtcggtctg gcgctgggcg atgacggtaa agttcgtgcg
     8101 ctggtggcgg cattaggtgg cggtttctgg acgtggtggt tctggcttgg tgtcgccggg
     8161 ctggggctga ttgtgccaat gttgctcaaa ccgtgggtca atcgcagttc cggcattcct
     8221 gccgtgctgg cggcgtgtgg ggccagtctg gtcggcgtgt tgatgctgcg ctttttcatt
     8281 ctctacgccg gacagttgac ggtggcgtaa gccagaaaag aggtggtttc tggacgtatt
     8341 ccttcctgaa gtcggttttc tggcgttgtt gttaagtctc ggggtcaacg tgttgacccc
     8401 gttgacggcc tttgcgggag tgcggttgcg ctggcctgcc atgatgcgac tcacttgcat
     8461 cggcattctg gcgcagttcg cgctcctgct gctcgccttt ggcgtactga cgtattgttt
     8521 tctcatcagc gatttctcgg tcatttatgt cgcgcaacat agctatagcc tgctgtcgtg
     8581 ggaattcaag ctggcagcgg tgtggggcgg ccatgaaggt tcgctgctgc tttgggtgct
     8641 gctgctttcc gcctggagcg cgctgtttgc ctggcattat cggcagcaaa ccgatccgct
     8701 atttccgctg acgttagccg ttttatctct catgctcgcc gcactgctac tgtttgtggt
     8761 gctgtggtcc gatcccttcg tgcggatatt tccaccagca atcgaaggcc gcgatctcaa
     8821 tccgatgctg caacatcccg gtcttatctt tcatccaccg ctgctttacc ttggctatgg
     8881 cggtttgatg gtagcggcga gcgtggcgct ggcgagtcta ctgcgcggcg agtttgatgc
     8941 ggccagcgcc cgaatttgct ggcgctgggc gttacctggc tggagtgcat taacggcggg
     9001 gatcatcctc ggttcctggt gggcctactg cgaactcggc tggggcggct ggtggttctg
     9061 ggatccggtg gaaaatgcct ctttattacc ctggctttct gccactgcgc tgctgcacag
     9121 tttatccctg acacgccagc gggggatttt ttgccactgg tcgctgttgc tggcgatagt
     9181 tactctgatg ctgtcgctgc tgggcacctt aattgtccgt tctggcattc tggtttcggt
     9241 tcatgcgttc gcactggata acgtccgcgc cgtgccgttg ttcagcctgt ttgcactgat
     9301 tagccttgcg tctttggctc tgtatggctg gcgagcgcgg gacggtggcc cggcggtgcg
     9361 tttttcgggg ttatcgcggg aaatgttaat cctcgctacg ctgttgctgt tttgcgcagt
     9421 gctactgatc gtgctggtgg gaacgcttta tccgatgatt tacggtctgc taggctgggg
     9481 acgcctctcc gttggcgcgc cgtattttaa ccgcgcgacg ttaccgtttg gtctgttgat
     9541 gctggtggtg attgtgctgg cgacgtttgt ctctggcaaa cgcgcgcagc ttccggcgct
     9601 ggtcgctcat gcgggcgtgc tgttatttgc cgcagggatc gtggtttcca gcgtcagccg
     9661 tcaggagatc agcctgaatt tacagccggg tcagcaggtg acgctggcag gatacacctt
     9721 ccgttttgag cgcctcgatc tgcaagccaa aggcaattac accagcgaaa aagcgatagt
     9781 ggcgctgttt gaccatcagc aacgcattgg tgaattaacg ccggagcggc gtttttatga
     9841 agcacgccgt cagcaaatga tggaaccgtc aattcgctgg aacggcatcc atgactggta
     9901 tgcggtcatg ggtgaaaaaa ccggagcgga tcgttacgct tttcgtttgt atgtacaaag
     9961 cggtgtgcgc tggatctggg gtggaggatt gttgatgatt gcgggcgcat tgttaagcgg
    10021 atggcggggg aagaagcgcg atgaataaag ggcttctcac gctactgctg ttatttacct
    10081 gttttgcgcg cgctcaggtc gtagacacct ggcaattcgc caatccgcaa caacagcaac
    10141 aggcgttaaa tattgccagc cagttacgtt gtccgcagtg ccagaatcaa aacttactcg
    10201 aatccaatgc gccggtggcg gtcagtatgc gccatcaggt ttatagcatg gtgtcggagg
    10261 ggaagaacga agtcgaaatc attggctgga tgaccgaacg ctacggagat tttgttcgct
    10321 ataacccacc gttaacgggt cagacgctgg tgttgtgggc gctgccagtg gtgttgttat
    10381 tgctgatggc actgatcctc tggcgagtga gggctaagcg atgaaacagc ctcaaatacc
    10441 ggtgaaaatg ctgacaacac tcacgatatt gatggtattc ctctgtgtcg gcagttatct
    10501 gttaagccca aaatggcagg cggtacgtgc ggagtatcag cgtcagcgcg atccgctaca
    10561 tcagtttgcc agccagcaaa ccccggaagc gcagcttcag gcattgcagg ataaaatccg
    10621 cgctaatcca caaaacagcg aacaatgggc gttactgggc gagtactatc tgtggcaaaa
    10681 cgattacagc aattcgctgc tggcgtatcg tcaggcgttg caactacgtg gtgagaacgc
    10741 tgaactgtat gcggcgctgg cgacggtgct ttattaccag gccagccagc atatgaccgc
    10801 ccagacttgc gcaatgatcg acaaagccct cgcgctggac tctaacgaaa tcaccgctct
    10861 gatgctgctg gcttccgatg cgtttatgca ggcgaattac gcgcaagcca tcgaactatg
    10921 gcaaaaagtg atggatctca actcaccccg gatcaaccgg acacagctgg tcgagtcgat
    10981 taatatggca aaactgttgc agcggagatc ggattaatca tctggtgatg aagattatgt
    11041 cattgagctg cataaaaaat aatcgaatga acatatgcca aaaataatca ctaatcagta
    11101 ttattgcaga ttaaacaaat aaaaatcttt ccataacaaa tggttattca ttaatcctgc
    11161 cataaactcg cgttattatg cattaatgca gcgaaaagct ctgttgttaa agggttgcgc
    11221 aacataccgc gcaaatgata ctgatcataa gcgttaaaaa aatctacaaa ccaacgcaac
    11281 acaattcatg ccctggcagt atgtcacgtt ctcgcgtttc tgaacgggga acggcgctcc
    11341 attgaggaag ttattctgat gaaaaatata aaattcagcc tggcctggca gattctgttt
    11401 gctatggtgc tgggcattct cctgggaagc tacctgcact accatagcga cagccgcgac
    11461 tggctggtcg tcaatttgct ctctccggcg ggtgatatct tcatccatct gatcaaaatg
    11521 attgttgtgc cgattgtgat ctccacgctg gtggtgggta tcgcgggtgt tggtgatgcc
    11581 aaacagctcg ggcgtattgg cgcgaaaacc attatttact tcgaggtgat caccaccgtc
    11641 gccatcattt tggggatcac tctggcgaac gtcttccagc ccggtgccgg ggtggatatg
    11701 tcgcagctgg cgaccgtcga tatctcgaaa tatcagagca ctacggaagc ggtacaaagc
    11761 agttcccacg gcattatggg cacgattttg tcgctggtgc cgacgaacat tgtggcgtcg
    11821 atggcgaaag gcgaaatgct gccgatcatc tttttctcgg tgctgtttgg tctggggctt
    11881 tcttccctgc ccgcgacgca tcgtgaaccg ctggtgaccg tgttccgctc catctctgaa
    11941 accatgttta aagtgactca catggtgatg cgttatgcac cggtgggtgt gtttgcgctg
    12001 attgcggtga cggtggctaa ctttggtttc tcttctctgt ggccactggc gaaactggtg
    12061 ctgctggtgc atttcgccat tctgttcttc gcgctggtag tgctgggaat tgtggcgcgc
    12121 ctgtgcgggt taagcgtctg gatcctgatt cgtattctga aagatgagct gattctggcg
    12181 tactccactg ccagctctga aagcgtactg ccgcgaatta ttgagaagat ggaagcctac
    12241 ggagcaccgg cgtcgatcac cagcttcgtg gtgccgaccg gttactcttt taaccttgat
    12301 ggttcgacgc tgtatcaaag tattgccgct attttcatcg ctcagctgta tggcattgac
    12361 ctgtccatct ggcaggaaat cattctggtg ctgacgctga tggtgacctc gaaagggatt
    12421 gctggcgtgc cgggcgtgtc gtttgtggtg ctgctggcaa cgctgggtag cgtaggtatt
    12481 ccgctggaag gtctggcgtt tattgctggt gttgaccgta tcctcgacat ggcgcgtact
    12541 gcgctgaacg tggtgggtaa tgcgctggcg gtgctggtga ttgccaagtg ggaacacaaa
    12601 tttgaccgca aaaaggcgct ggcttatgag cgtgaagtgc tgggcaaatt tgataaaact
    12661 gcggatcaat aattgaagat tgccggggat atccacccgg caatgtgtga atgcctgatg
    12721 cgacgcttgc cgcgtcttat caggcctacc ttaaccgttg gttaattttt caaactcttc
    12781 acacccggta tcaaaccctt ccatacagct caggttcaac cagtgcagcg ccttttgctt
    12841 gttcttctcg ataaaacctt cttcaccgtt taagaacatc attcccgccc agtactcgga
    12901 ataaccggtt cgggaaattg cagagctgcg tttgaaatac caggttgcct tgtcatcgtc
    12961 ggccttaatg cccacgccgt tggcgtaaat caaaccaagc agcatttggg catcgacggc
    13021 agagtcgttc tccagatctt ccgaggcgtt ttctaacagc gaaatagcct ttggataatc
    13081 cggtttaccc gcttgagtat ttaccagaat atgcgccagc gttacttcac cttctttact
    13141 gcccgcttgc gccgctttct ctgcaagaac ttttgcttgt ggatagtcca gactgaccgg
    13201 attggtgatt ttgatctgcg ccagcagcgc gcaggcatca gcatcaccgc tgtcggcggc
    13261 tttctgtgcc caatattcgg ctttgctcaa atcgccggag ctaaaccagc tgtcggcaag
    13321 aaaatattgt gcgcgtcggt ccccggcctc tgctgccttt aaatactggc tgccaggctc
    13381 ggagtcgttg gcgtgggcaa agaatgtcaa aaacaacatc aatgcgataa tttttttcat
    13441 tttgataata cgattgggta gggagtaacc agtataaaga gttcgaatgg ataaaaaaac
    13501 agcctccgaa aggaggctgt agaaaggact gtattacgcc agtgccgctt cgcgcaggcg
    13561 agttttcaac ttgttgtact cgtcaatcac gtactgctcg gcggcgcgct gatcggcgat
    13621 cggctcgacg cgcacggcgc agtatttgta ttccggcgtt ttcgtaatcg ggcttaagtt
    13681 ttcggtaacc agctcgttac aggcaccaat ccaccactgg taggtcatgt aaatcgcccc
    13741 tttgttcgga cgatcgctga cctgcgcacg ggtgataatt ttgcctttac gcgagtgcac
    13801 ccaaaccaat gcctcatctt caatacccag acgtttggcg tcttcggtat tgatttgtgc
    13861 gtagccaggt tcatcagcca gcgccgccag tgccgcacag ttaccggtca tcgaacggca
    13921 agagtagtga ccaacttcac gcaccgttga cagtaccatc gggtactcgt cggtgagttt
    13981 gtcgattggc gctacccagt cgcaggtgaa gaactgcgcc agaccgttcg gggtatcaaa
    14041 cttctcttta aacagataag aagtcccctg atcggcatct gaagtatcgc ggcaaggcca
    14101 ctgaatgaag cccagttcgc ccattttctc gtaagtcgca ccgtagaaat ccgggcacag
    14161 atgacgcaac tcatcccaga tctcctgggt gttgttgtag tgcatcggat aacccatacg
    14221 ggtggcgatt tcactgatga tttgccagtc cgttttcaga tcccatttcg gttcaactgc
    14281 tttgaagaaa cgctggaagc cacggtcagc cgcagtaaac acgccttcat gctcgcccca
    14341 cgacgttgac ggcaaaatga catccgccgc cgacgcggtt ttggtcataa agatgtcctg
    14401 aacgataacc agttccagat cttcaaaggc tttacgtact gccgacagct ccgcgtcagt
    14461 ttgcagcgga tcttcgccca taatgtacgc ggcacgcact tcgccatgcg ctgcgcggtg
    14521 cggcagctcg ctgatgcgat aaccggtatg agcaggcagg ctttccacgc cccaggcttt
    14581 ggcgaatttc tcgcggttag ccggatcttt cacgtactgg tatcccggat acgtatccgg
    14641 cagcgcgccc atatcgcagg caccctgcac gttgttctga ccacgaaccg ggtttacacc
    14701 cgcatgcggc ttaccgaggt taccagtcag catcgcgagg ctggtcagag aacgcacggt
    14761 ttccacgccc tggtagaact gggttacacc catgccccac aggatggcgg cgcttttcgc
    14821 ctgggcatac atccgtgccg cctgacgaat ctcactggcg ctgacgccgg tgatatcttc
    14881 aaccgactcc ggcgtgtagc cttcaacgat tttacgatac tcttcaaagc cttctgtacg
    14941 ggaagcgacg aacgctttgt cgtacagatt ttcttcaata atgacatggc ccatcgcatt
    15001 caacagcgcg atgttcgagc cgtttttcag tgcaatgtgc atgtcagcaa tgcgcgcggt
    15061 ttcaattttg cgcggatcgc agacgataat tttcgccccg ttacgtttag cgttaattac
    15121 gtgattcgcc acgattgggt gggaatcagc cgggttgtac ccgaaaacga acactaaatc
    15181 ggtattatca atttcgttaa tagcattgct cattgcgcca ttaccgaccg attggtgcag
    15241 acctgcaacc gatgggccgt gtcagacacg agcgcagcag tcaacgttat tggtaccaat
    15301 aacggcgcgc gcaaattttt gcattacata gttggtttcg ttacccgtac cacgcgagga
    15361 gccggtcgtc tggatggcat ccggaccgta cttctctttg atggcgctca ggcgctcggt
    15421 aacgtaattc agtgcctcat cccaggaaac aggttcgagt ttgccgccac gctggcgacg
    15481 gatcatgggg gttttcaggc gcggggtcag gatctgggta tcgttaatga aatcccagcc
    15541 ataataaccc ttcagacaca gggtaccctg gttggttttc ccctgcgctg cctccgcccg
    15601 gacgattttg ccgttatcga cgaccaggtt gattttgcaa cctgatgcgc aataggggca
    15661 aaccgtgacg acttttttca tcggtctcgc tccagttaat caaatcacgc atacgcgctc
    15721 tcgactacag tatgcatctt ttatgccaca ttttatttgg ggtcattccc tgatattacg
    15781 ggcactattt attcaaaact ctgacgaaaa acaggctgtc gtcagttttg acgtgacgaa
    15841 acgaaatacc gcgtgacagc catcacgcgg cagacatttt attttttctc gacgacggga
    15901 gccgcctgat acccgccgcc cagcgatttc atcaactgaa tgctttggat cacccggcgg
    15961 ctgtccagca tcagtaatga catctcttcg gcaagtactg gcaaccgggc ttcagtggcc
    16021 tgtaagcggc tggttaagcc gcgctgatag gcggcctcgg cagcgcgctg ggtgaagcgc
    16081 gttgcttcca cgcgttcagc ctgcatttct cgctcgtcgt tgagcgtttg cagacgcgtg
    16141 ccgttgacgg caacgtcacg caccgcgttc agtactgact ggttgtaacg ttcaatcatc
    16201 atgttgctgg cggcgcgcgt gccttcgaga ttggcattca accgtccacc gtcaaacagc
    16261 ggcaatttca gacccgggat gaagttgaac tggcgactgg tttttttgaa taaggtatcc
    16321 aggtggatgg catcaaggcc gaaaaacgct ttgatatcaa agctcgggta gaacagcgcc
    16381 cgcgcggaat ccacctgatc taatgacgcc tgaacatacc agcgcatggc ttgcagatcc
    16441 gggcgtctgg cgagcaactc ataagagagt gtcgccggaa tgccggtctg gactcgtggt
    16501 aatgccaccg gtttgatctc cggcatatcg ctggcccccg cgccaatcaa tgcacgcaga
    16561 gattctcgcg tttcggtgat ttgccctttg acggcagcaa tttgtttatc gaccgccaga
    16621 atctgtgccc gcgcgccgtg gaaaggcact tgtgcttcca gaccgtgcgc cactttactc
    16681 tggtgcgctt tcaccgcgta atcaatcaca tcgcgagttt gttctaacag atcgagcatc
    16741 tgatagctgg cctgcatact gtaataaagc tgcgctacgc ccgtggtcag cgatagctct
    16801 actgctgcgg tttctgccag cgcggcatta tgcgcgccaa tggcggcggc aaccgctgag
    16861 cgatgcacac cccacaaatc aagatccagt ccggcaaaca aacctactgt ggcttccgta
    16921 tagtacggcc cgtccatacc cagtgcgggc gcatccatcg cataagggct taaaaagccg
    16981 ttcgccgaga cgcgttggcg gttgagcatc cctaacgctg cgacctgtaa ttgtgaaccg
    17041 gcatctaaca aatcggcctg ggactgcgct ttttcttccc gcagtttcgc ttcggcgagg
    17101 gtgtgtgaac cacttagcgt ccgttggatc agcgcatcca gctgcgggtc attgagttgt
    17161 ttccaccact gcgcctgcgg ccagccggag ctggcaagat gaatatcatc ggccagtttg
    17221 atttgttccg gtttgagctg ttgatgaggc gcagaatcct tgcgtaccaa ggcacagccg
    17281 gaaatcagcg tcgtgctgcc gagaatgctg cacaacagca gacgtgaaag ttgacgattg
    17341 atcattgcgt ggctccttgt gcctgttccg tggcgggcgt taatgccggg gctgtccagt
    17401 ctggcaagcg ggcgaaaagc tggcagacgt gttgctcctg ttttaataag gtgggcagga
    17461 tggcctgcga ggcgggtgtc tcttcaagcg ttatttgagg agaacggctg agtgcggttg
    17521 ccagaccggc agcgtatttt tccagggcgt cggcaaactg acctgctctg tccggctgta
    17581 gtgcgttatc cagcgcctgc gccgaattcc aggtggcatc ccaggcgtga aggatatcgc
    17641 ggccctgacg aataaccgtt tgcgaacgtt caatcagtaa ggcgcgttct tcgctgtcca
    17701 gttgacgctc cagcgccacg cgttggcaca tctcttcaca ggcattaaac gccgcatgca
    17761 gaccgatacg aatttgcaga taagtgcgca gagccgtgac ttcctgctgg cgtgggatcc
    17821 gcattacttt acttaacata cccagcgcgc cagccagttt ttgcggcagc gtgcgcgctt
    17881 cactttcagg ccagacaaag gtgtaaatca ccgcggacac cacggtacca atgatgatac
    17941 ccagggcgcg atcgcgaatt tccaccaggt cgtacactgg gccaaaaacg ttttcgagcg
    18001 tggcgagcgc gaaggtgacc accatctgtg tgccgatata agaagagcgt tcagagctgg
    18061 tggcaatcca tgcgcccaac aggaaaatcg gtgccagcac aaacagcaat tcgacaatat
    18121 tgtccagcca gggcatgacc agtagcgtga ataacagcgc cagaatcgcg ccgcaaaagg
    18181 ccccgccaaa acgcagcacc atcttctggt acgacgaacc gacatttgga ttagagacga
    18241 tcacgcaggt cagcatacag gtgtgaatgc cttcccagtc cacgccgctg taaaaggtgt
    18301 aacagatcaa acaggcgagc aatgttttta ccgcgtagcg tatatagtct ggattggtaa
    18361 aagcatcggc gaccattgat ggcggtttgg cggcgggcgt tggcggcgta ttcgggttca
    18421 tctggcccag ttgtaacaac gtctggcaga tattctccag gttacattcc cgtgccgcca
    18481 ccgcttcact ttcactgatc cgccagtcgc tttgccagca ctgaccttca gcaacggcat
    18541 gctgcagctt gttgatttct gaggctaatt tttgccgaaa ttcaataatt gcctgagaat
    18601 cagcaaaaga ggtgggatcg tagcgattca gcgtcgagta aatgtaggtt accgttgcca
    18661 cgcagctttg ccaccatgcg ctttgagttc gccagttggc atcgtccgcg agacaaaaga
    18721 cattcagttt ttgcagcgcc agcgcctctc tttcaatccg cgtttcgggt agcggtgcga
    18781 ggctgtctgt caggtgacta atggcatcat caagccgatc attaagcgcc tgatgcattt
    18841 gcgaaatggc acgactggga aaccacagca cgccgattaa cgtcatcagc aaggttggat
    18901 agaggccaac aacgatacac cacagcgtta agcgcacgac cacttccgga tagtcgagca
    18961 tggcggggaa ggtttgcccg taaatggcga caatggcgac agcgaaaaag accagtccca
    19021 gccgatgggt gcgcatcaaa aacatgcggc ccatcaggat cggcccggcg atgatcaatc
    19081 ggatcaacgg ttcgccgtat gaccatttgt agatcaaaaa caggctgccg atctccagca
    19141 ccgtggcaac cacaaacaag atcgccacaa atttggtgta aaacgcgttc gactgaatac
    19201 cgtaaaacag cactgccagc gataacgcca caaaagggat ctcaaaagtc atcgagatca
    19261 gaatcaccag caggcagcct acccagagtt gcacgatctg cggcacgcgg ccgggtcgcc
    19321 gctcgcttaa ctcttcatga aagaacgcca gcagcctgac catcggtaat ggcagggagt
    19381 tgagcgcgct cattattgcg gctcaagatt agcgactgcc gaagcgccga tgcggaacat
    19441 ttccgggtca ggtttatcga ccatgatttt gaccggaaaa cgctgggcaa cgcggaccca
    19501 gttaatagag cgagaaactt tcggcaggcc gcccagcacc aggccgccgt catccggtag
    19561 cacgccgtag ccaatcgaat ccactttacc ctcgaaggtt ttgccgctgt cactcatcag
    19621 gcgaatcgtt gcgggtgtac ctgagcgaat atttttcaga tcggtttcgc ggaagttggc
    19681 gatcacgtac cagtgacgag tgtcgattag ggtaaaaata gggcgcatgg cagaagcaaa
    19741 ttgcccgacg gaggttttga gggaaatgac ccggccatca aacggcgcgc gaacggtcgc
    19801 catttccaga tgcagtttgg tcagggcaat atccgcttcg accgccgcac gttgcgccac
    19861 cagcgcatcg acgccgctga cggcgctggc ggctgactgc gcctgtaaca atacggcatt
    19921 aagatcggct tctgcggcgc gctgcgcagt tcttgcacgg tcaacgtctt ccgctgagac
    19981 aaaaccctct ttcagtaatg gctcggtacg gcgtaatgta tctgtggcct gtttcgcggc
    20041 ggcacgggct ttttctaccg tggcattaat cgagtcggca ccaaactgtt gcgcatcaac
    20101 gctacgctgg gtgagcataa tttgcttatc cagcgccgcg agggaggctt cagctttcgc
    20161 cagattggct tcgtacgggc gcgggtcaat gcggaacagc aaatcgccct gtttgactgc
    20221 ctggttgtcg gtgaccgcca gttctacaat gcggccgctg acttccggca ccacatcaat
    20281 ggtatctgct gacgcgtaag cgtcattagt tgatggcgca ctgtctacgc gccagataac
    20341 gaaaacaagg gcaaccagcg ccaatgcaac cactaacaga gcagggaatt tactgcgagg
    20401 agcttttttc ggcgtacttt ccatgagcgc gtctcaaata gattagaaaa atgccagcca
    20461 gaacagcatg gcgtagagag caaaaagggc ggtataaata attccgacaa atgccagatt
    20521 gatatttgcc cgctgaataa ctcgcctcgt gatgagcatc aaaataaggc tggcaatggc
    20581 gcagaaaaac cagctgggat aataagcacc gatcaccggg attgccggag ataatgagca
    20641 tcccgaaacc atgaagacgg gaattatcca ggctgttttt ttgagttgca tcgccatgcg
    20701 agagagaact gttggcatat taaatacatc ctgtctgctg cgaacaatat ctgtagtgaa
    20761 tatcaatatc cattgaaggc gtcagtatgg ctattttctc gatgaataaa aatagcttta
    20821 ttcagtcacc gcattcataa cttatatatt ttatttatta attggctgta tatattttta
    20881 aaataaatgc ttttgggggc ttcagcgccc ccaaaagcga gtgacattaa tttggggtta
    20941 cgatattcac ccacaaatcg aaattatcca gacaggcgat aatttcttcc agtttcgcgc
    21001 cattgccaat aattttggct ttcttcgctt ttaccagatc cgccattttg gcttgcccgg
    21061 tcagcacggc gtgcagatct tcacggctga tgtagaaaga ggcgtcagct tgggattgca
    21121 gtgttttacg gtagttaagc acgctatcgt tcagcgtcag gttgaggtta tcgccattgc
    21181 tcatattgaa gttcaggctg atatttttac ccgcggcttt cgcgctatcg aggcgaacgg
    21241 acataaagtc gaacagcatt tcgaccgaca tcccgcgaat ggtgtccggg gaaccgatgg
    21301 tgccgtggct gaacttatgc accccttcgc gcagctcttt cgcgccggtc aggtagaaac
    21361 cgcgccaggt ggcggactcg gcctgatagc ccagctgttc aaagttattc gcttgcagat
    21421 tcttcgcgac ctggtcaccc gggttggcgg caatcacctg tttcagcagt tctgccgacc
    21481 agcggtaatc accttgcttg ttcgcttctt gcgccaggtt gatgacacgg gcagaaccgc
    21541 ccagcgcctg cacgtaacgt ttacccatct ccacctgacc atacggatgc aggttagccg
    21601 ggttaccgtc gtaatagcca agatagaagt tatacaccgc gcgggcgttg tggctgacag
    21661 aaccgtaata gccacggctg gcccagttat tggcaagtgc aggcggcagt ttaatcatgt
    21721 cgccgatttc attcatggtg tagccctggt tcgccaggtg cagggtctgg tcgtgaatgt
    21781 acttgatagt atcgcgatat ttaccaatat aatcattgat atgcttattg ccccagaccg
    21841 gccaggtgtg cggcataaac aggacttccg cgtcgttacc ccacatatcc agcgtttcgt
    21901 tcagatactc ggtccacttg ctggtgtcgc gggttttcgc gccgcgcaga gtgtagaagt
    21961 tgtgcagggt atgcgtggcg ttctcggcgg tacacagcgc tttcagggcc ggaatataga
    22021 agtgcatttc ggctggtgct tcgctacctg gggtcatcag gaagtcaaac tccaggccgt
    22081 cgataatcat cttctcaccg gttttggtga tggttttgtt cggtgcgatg atgctcgggt
    22141 agcccgatgc cagcgttacg ccaagaccat tgccgatgtt accctgaggg ttatgcgcca
    22201 gcagcaggcc gtaggagtaa agtgcacggc ggctcataat attacccgcc agtacgtttt
    22261 cgctgatggc ttcgtccata aagccagcag gggcgataac ctgcacttta ccggatttca
    22321 catcagcttc agagataatg cctttcacgc cgccatagtg gtcggtatgg ctgtgggtat
    22381 aaataaccgc aacaatcggt ttttgcgggc gattttggaa gtaaaggtca agggcggctt
    22441 ttgcgctcgg tggcgtcacc agcgggtcga tgacaataat cccggtatca ccttcgataa
    22501 aggtgatgtt ggagatatcc tgaccgcgaa cctggtacat tctgtcggtg actttaaaca
    22561 ggccggaaat accgtttaac tgcgactgac gccacaggct agggttaacg gtttcgggcg
    22621 cgggcgcatt gatatcaaat ttataatctt ccccgcgata atatggtttg ccattagcat
    22681 cgcgcagaat accttcatcc agcagcggag cgataaatcc acgctgagcg tcgtcaaaat
    22741 cctgacgatc gctaaacggt aatgatttgg cgtaattctg attaacctgt tgggtatatt
    22801 gagtggcggc tttcgcttcc tctttcgcgt tagcggtatt taccatcatg ccgctggcgg
    22861 cagttaacgc aataacaatt ctgctcaaac ggaataaccg agagttattc atgtaaatct
    22921 ccatttatca tttatgtcgt aaatatgtaa tccatgcaaa atacgtcatt taaagctggg
    22981 gttatcataa ttgggaattt gcgactttaa taagtggaag tgtgagctga acgatccatt
    23041 ttattaggca tttgtgctga agagggaaaa ttacgcgaat ttattatctc tgtaaataac
    23101 atgaggtgtt ttatatattt attatatata ttgtacttcg gcaaatactg agcacaattt
    23161 atcaatgcaa ccggtgtttc tttttaaaaa acgtaccgga gcaatgggcg gtcatgtcgc
    23221 tggcaccgca ggattctgcg ttgctgtgcc agccttttat tcatggtcaa tgagtgaggt
    23281 gattgataaa gacaccaggt cgtcagagcg cactgattat ccgcattatg ccagtgaagt
    23341 tgcactggct tttggttcac gagtggttgg cctcgaactg gcaaaaatga ttgtggatgc
    23401 gtggctgggc gcacagtacg aaggcggtcg tcatcaacaa cgcgtggagg cgattacggc
    23461 aatagagcag cggagaaatt gagattcatc cactacttgc atggatgagt aatgactaat
    23521 gtggatagag tttctttttg aggttggcta atgaaacgct ttccgctatt tcttttattc
    23581 accctgctca cgctgtccac cgttccggcc caggctgata ttatcgacga tactattggt
    23641 aatattcagc aggcgattaa tgacgcttat aaccctgacc gtgggcgaga ctatgaagat
    23701 tcgcgcgacg acggctggca gcgtgaagtg agcgacgatc ggcgcagaca atatgacgac
    23761 cgccgccgcc agtttgaaga tcgccgcagg cagctggacg atcgccagcg ccagcttgat
    23821 caggagcgtc ggcaactgga ggatgaagag cggagaatgg aagatgagta tgggcgatga
    23881 ggttgggtat ggagcaggca tcgcccctca ccccaatggg gagagggaga aaacgagcgc
    23941 aatattcaat attgagcaca atcggtcccc tcgcccctct ggggagaggg ttagggtgag
    24001 ggggccagcg cgcacattcc ctctcatcac gccaccccaa tctccatccc atcaaacccc
    24061 acctcaaacc ctgacggtag tgcgttttcc atcagccacg catcaaactg gtggctgata
    24121 tgggtcagaa tcacccgtgg cgagcggata acctgattca gcgcaagcac ggtatttaaa
    24181 tcacagtgat tacgcggtgc atccgcgcgc ggcgggtgac tgcaatccat caccattacc
    24241 tgcggctgat tattgcgtaa aaatttcagc gttttttccg gcaagccagc ggtgtcagac
    24301 agccacgcca cccggctgtg cgccgtttcc agtaaatagc cgaaagtgag ttttgagtgg
    24361 ttaagcggca gcggcgtgac ctgcaacccc tgcaaatcaa acaccacaaa cggttccacc
    24421 gtgtggctga aatcaagcag cccgggatgt ttaaacagat cgtcgcagcc ctgttcatcc
    24481 ggcgggccgt agatcgggat cggatcgccc acgccccagc gcagcggaaa cagcccctgg
    24541 acgtgatcca tatgataatg cgtcagcaaa aactgctgga acgatccggg agaccagcga
    24601 tcggtgagat cgtgcagccc g
//
LOCUS       DAJTZL010000044        24485 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.044, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000044 DAJTZL010000000
VERSION     DAJTZL010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24485
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.044"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..348)
                     /gene="rrrD"
                     /locus_tag="OO544_001592"
     CDS             complement(<1..348)
                     /gene="rrrD"
                     /locus_tag="OO544_001592"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HCR6767214.1"
                     /translation="MSPSLRKAVAAAIGGGAVAIASVLITGPSGNDGLEGVSYIPYKD
                     IVGVWTVCYGHTGKDIMLGKTYTEAECKALLNKDLATVARQINPYIKVDIPETTRGAL
                     YSFVYNVGAGNFRT"
     gene            complement(348..563)
                     /gene="essD"
                     /locus_tag="OO544_001593"
     CDS             complement(348..563)
                     /gene="essD"
                     /locus_tag="OO544_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HCR6767215.1"
                     /translation="MKSMDKLTTGVAYGTSAGSAGYWFLQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGE"
     gene            complement(631..1695)
                     /locus_tag="OO544_001594"
     CDS             complement(631..1695)
                     /locus_tag="OO544_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HCR6767216.1"
                     /translation="MKNTVKINSVDLINADCLHFIQSLPDDSIDLIVTDPPYFKVKPN
                     GWDNQWKGDEDYLKWLDHCLAQFWRVLKPAGSLYLFCGHRLASDIEIMMRERFNVLNH
                     IIWAKPSGRWNGCNKESLRAYFPATERVLFAEHYQGPYRGKSDGYAAKESQLKQHIMA
                     PLISYFRDARAELGITAKQIAEATGKKNMVSHWFGASQWQLPNEADYRKLQALFSRIA
                     AEKFQEQQLEQQLEQPHHQLVASYDSLNRKYSELLDEFKTLRRYFSVSVSVPYTDVWT
                     HKPVQFYPGKHPCEKPADMLRQIINASSRPGDLVADFFMGSGSTIKAAMALGRRALGV
                     ELESERFNQTVKEINELVGK"
     gene            complement(1845..2039)
                     /locus_tag="OO544_001595"
     CDS             complement(1845..2039)
                     /locus_tag="OO544_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001317671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HCR6767217.1"
                     /translation="MLKQQDMTETAKVVFNELSIEPATVGEIAQNTYLSRERCQLILT
                     QLVMAGLADYQFGCYRRLQQ"
     gene            2312..3643
                     /locus_tag="OO544_001596"
     CDS             2312..3643
                     /locus_tag="OO544_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTPase"
                     /protein_id="HCR6767218.1"
                     /translation="MLDTLSFTERDEFQRRNIAENIIKLLKPEADISPLVIDGAWGTG
                     KTEFSIKLKNLIIEQETESKVVYIDAFKGDHAESPLLLITSAIASILPEEEKQHFIKR
                     SLPAIRFGLKTVLKAGAGWFLRQEASEVAEEFQDAMKKASNAAIDGTIENLLEDHMDS
                     EKNINSLKSCIESISKNQKIVIIIDELDRCKPSFSTNVIETIKHIFDINNVFFILVTN
                     TEQLKASINHIYGYSINSQKYLDKFIKYTITLPDTCLINGHNVCKASVIYWDYLVEKT
                     RLLNKINRLSGYFIRDLIQRTNLSLRETQTFSRNLNIFQLLNDDENKSSDPLINMIFV
                     VAAFIHCFGNKEKLKQEITAESISYLADLLNIKEIPYSYERRSQIPEISIVFFGIIKD
                     SITLNERFAPKSDEELKKFTNVYTDYEHINFWSTTPRELLIKYINQMSFIQ"
     gene            complement(3672..4040)
                     /locus_tag="OO544_001597"
     CDS             complement(3672..4040)
                     /locus_tag="OO544_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein Q"
                     /protein_id="HCR6767219.1"
                     /translation="MMRDIQMVLERWGAWAASDSSGVDYSPIAAGFKGLLPYTSKKRL
                     ACSDSDALIIEGCLARLKQKRPDEHSLLVAHYLYGISKRKLAKARKKDEKLIRIEIQL
                     AEGFIDGCLSMLDLTLDLDV"
     gene            complement(4055..5044)
                     /locus_tag="OO544_001598"
     CDS             complement(4055..5044)
                     /locus_tag="OO544_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCR6767220.1"
                     /translation="MRALLTPEIAPRMGIVLFRPGSELMPLFMQGRVLLEPEPERYSS
                     FASGAVPAASQPLADDPAVRAVFRNEAVIRRAGGVECLESWLLREKGCQWPHSNWHSE
                     NMTTMRHAPGAIRLCWHCDNQLRDQFTERLESMATDNCARWVLSVVRRDLGFDDSHVV
                     TMPELCWWLVRNDLADALPESAARKALRLPKPVVPSVTRESDLVPSVPATSIMQDKAK
                     KVLALKVEPESPESFMLRPKRRRWVNEKYTRWVKTQPCACCGKPADDPHHLIGHGQGG
                     MSTKAHDLFVLPLCRKHHDELHADTVAFEEKYGSQLELIFRFIDRVLAIGVLA"
     gene            complement(5052..5861)
                     /locus_tag="OO544_001599"
     CDS             complement(5052..5861)
                     /locus_tag="OO544_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HCR6767221.1"
                     /translation="MNNLMVIDGIEVRRDAYGRYSLNDLHRAAVASGANARTKEPGKF
                     LSSQQTVELVHELTNTQNLGVDPVSVIHGGNERGTYVCKELVYAYAMWISPSFHLKVI
                     RTFDMVTSAPEKLSGQAADKMQAGVILLDFMRRELNLSNSSVLGACQKLQEAVGLPNL
                     APRYAIDAPADAPDGSSRPTLSLSALLKQYGIRLTANQAYHQMAKLGIVEQRERYSRT
                     AINNIKKFWSLTAKGCMFGKNITSPANPRETQPHFFESRFPELLKLLDTVH"
     gene            complement(5881..6270)
                     /locus_tag="OO544_001600"
     CDS             complement(5881..6270)
                     /locus_tag="OO544_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCR6767222.1"
                     /translation="MKLILPFPPSVNTYWRHPNKGAFAGKSLISAAGRKFQSAACAAI
                     VEQLRRLPKPTSAPASVEIVLFPPDNRIRDLDNYNKALFDALTHAGVWEDDSQVKRML
                     VEWGPVIPEGKVEITISKYEKTAGAAA"
     gene            complement(6267..6593)
                     /locus_tag="OO544_001601"
     CDS             complement(6267..6593)
                     /locus_tag="OO544_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HCR6767223.1"
                     /translation="MTTLTQCQQQVLDMLISYQKERGFPPTNQEVATMLGYRSVNAAV
                     EHLRALEKKGVITIKRGVARGITLHTAVKDDDSEAVGIIRSLLAGEENARLRATHWLH
                     KRGLKV"
     gene            complement(6590..7243)
                     /locus_tag="OO544_001602"
     CDS             complement(6590..7243)
                     /locus_tag="OO544_001602"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001378271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HCR6767224.1"
                     /translation="MSNKYCQALVELRNKPAHELKEVGDQWRTPDNIFWGINTLFGPF
                     VLDLFTDGDNTKCAAYYTAEDNALAHDWSERLAELKGAAFGNPPYSRASQHEGQYITG
                     MRYIMKHASAMRDKGGRYVFLIKAATSEVWWPEDADHIAFIRGRIGFELPVWFIPKDE
                     KQVPTGAFFAGAIAVFDKTWKGSAISYIGRDELEACGEAFLAQVRQQAEKLVREMAA"
     gene            complement(7243..7737)
                     /locus_tag="OO544_001603"
     CDS             complement(7243..7737)
                     /locus_tag="OO544_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HCR6767225.1"
                     /translation="MSMSLLNDVQKFIEAHPGCTSGDIADAFAGYSRQRVLQSASKLR
                     QSGRVAHRCEGDTHRHFPRLTERAQDPEPQPVRETRPVRNFYVGTNDPRVILCLTRQA
                     EELESRGLYRRAATVWMAAFRESHSQPERNNFLVRRERCLRKSSKRAASGEEWYLSGN
                     YVGA"
     gene            complement(7734..8675)
                     /locus_tag="OO544_001604"
     CDS             complement(7734..8675)
                     /locus_tag="OO544_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6767226.1"
                     /translation="MSTKLTGYVWDGCAASGMKLSSVAIMARLADFSNDEGVCWPSIE
                     TIARQIGAGMSTVRTAIARLEAEGWLTRKARRQGNRNASNVYQLNVAKLQAAAFSQLS
                     DSDPSKSDASKSDPSKFDASKSGKKAGFHPSESGGDPSVKSKHDPSDKKPSRPDASQP
                     DTQTAEQYFLTRHPDAVVFSPKKRQWGTQDDLTCAQWLWKKIIALYEQAAECDGEVVR
                     PKEPNWTAWANEIRLMCVQDGRTHKQICEMYSRVSRDPFWCRNVLSPSKLREKWDELS
                     LRLSPSVSTYTEKREDPYFKASYDNVDYSQIPAGFRG"
     gene            complement(8665..8844)
                     /locus_tag="OO544_001605"
     CDS             complement(8665..8844)
                     /locus_tag="OO544_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001761574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HCR6767227.1"
                     /translation="MRQVNRWFKDHYGVPVRVIRWEPETQRVIYLREGYEHECFSPLE
                     QFRRKFREIEVGHEH"
     gene            complement(9020..9577)
                     /gene="ymfL"
                     /locus_tag="OO544_001606"
     CDS             complement(9020..9577)
                     /gene="ymfL"
                     /locus_tag="OO544_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmfL"
                     /protein_id="HCR6767228.1"
                     /translation="MGNHHWKVEKQPEWYVKAVRKTIAALPGGYAEAAEWLDVTENAL
                     FNRLRADGDQIFPLGWAMILQRAGGTHFIADAVAQSANGVFVSLPDVEDVDNADINQR
                     LLEVIEQIGSYSKQIRSAIEDGVVEPHEKTAINDELYLSISKLQEHAALVYKIFCVSE
                     SNDARECAAPGVVASIASGCGETNA"
     gene            complement(9570..9830)
                     /locus_tag="OO544_001607"
     CDS             complement(9570..9830)
                     /locus_tag="OO544_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001191680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6767229.1"
                     /translation="MQSPLRNVRKAHGFTLQHVAAGVQVNPATLSRIERLEQIPSIDL
                     AERLANFFKGEISEMQILYPARFQSSQNQNGFKPQEQEVSRG"
     gene            9928..10620
                     /locus_tag="OO544_001608"
     CDS             9928..10620
                     /locus_tag="OO544_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001358750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6767230.1"
                     /translation="MNIGNRVRQLRQAKNMKIADLAEAIGVDAANISRLETGKQKQFT
                     EQALSNIARSLGVDIADLFTSDVKSNTVCKNSISEDVAQVKDVFRIEMLDVSASAGNG
                     LIQGGDVIDVIHAIEYRTDNAVSMFGGRPANHIKVINVRGDSMCPTIEPGDLIFVDVS
                     INQFDGDGIYVFGFDDKIYVKRLQMIPDKLLVISDNQIYREWGITSENEHRFMVFGKV
                     LISQSQTLKRHN"
     gene            11305..11685
                     /locus_tag="OO544_001609"
     CDS             11305..11685
                     /locus_tag="OO544_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6767231.1"
                     /translation="MMRSSKMASERSTDVQAFIGELDGGVFETKIGAVLSEVASGVMN
                     TKTKGKVSLNLEIEPFDENRVKIKHKLSYVRPTNRGKISEEDTTETPMYVNRGGRLTI
                     LQEDQGQLLTLAGEPDGKLRAAGH"
     gene            11751..12575
                     /locus_tag="OO544_001610"
     CDS             11751..12575
                     /locus_tag="OO544_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2303 family protein"
                     /protein_id="HCR6767232.1"
                     /translation="MSQNLDATAINQIHALISAQGVNEIISKIGADAVALPENFRIHD
                     LEKFNLNRFRFRGALSTASIDDFTRYSKDLADEGTRCFIDADNMRAVSVLNLGTIDEP
                     GHADNTATLKLKKTAPFSALLSVNGERHSQKSLAEWIEDWADYLVGFDANGDAIQATK
                     AAAAVRKITIEANQTADFEDNDFSGKRSLMESVEAKTKDIMPVAFEFKCVPFEGLKER
                     PFKLRLSIITGDRPVLVLRIIQLEAVQEEMANEFRDLLVEKFKDSKVETFIGTFTA"
     gene            12703..13239
                     /gene="yfdR"
                     /locus_tag="OO544_001611"
     CDS             12703..13239
                     /gene="yfdR"
                     /locus_tag="OO544_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HCR6767233.1"
                     /translation="MSFIKTFSGKHFYYDRINKDDIVINDIAVSLSNICRFAGHLSHF
                     YSVAQHAVLCSQLVPQEFAFEALMHDATEAYCQDIPAPLKRLLPDYKRMEEKIDAVIR
                     EKYGLPTVMSTPVKYADLIMLATERRDLGLDDGSFWPVLEGIPATEMFKVIPLAPGHA
                     YGMFMERFNELSELRKCA"
     gene            13230..13592
                     /locus_tag="OO544_001612"
     CDS             13230..13592
                     /locus_tag="OO544_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6767234.1"
                     /translation="MRMNVFEMEGFLRGRCVPRDLKVNETDAEYLVRKFDALEAKCAA
                     LENKVIPVSTELPPANESVLLFDANGEGWLIGWRSLWYTWGQKETGEWQWTFQVGDLE
                     NVNITHWAVMPKAPEAGA"
     gene            13592..13897
                     /locus_tag="OO544_001613"
     CDS             13592..13897
                     /locus_tag="OO544_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6767235.1"
                     /translation="MTTFTDKELIKEIKERISSLDVRDDIERRAYEIALLSLEVEPDE
                     RESYELFMEKRFGDLVDRRRAKNGDNEYMAWDMTLGWIVWQQRAGIHFSTMSQQEVK"
     gene            14124..15287
                     /locus_tag="OO544_001614"
     CDS             14124..15287
                     /locus_tag="OO544_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR6767236.1"
                     /translation="MSLFRRGEIWYASFTLPNGKRFKQSLGTKDKRQATELHDKLKAE
                     AWRVSKLGEIPDITFEEACVRWLEEKAHKKSLDDDKSRIGFWLQHFAGMQLRDITESK
                     IYSAMQKMTNRRHEENWKLRAEACRKKGKPVPEYTPKPASVATKATHLSFIKALLRAA
                     EREWKMLDKAPIIKVPQPKNKRIRWLEPHEAQRLIDECPEPLKSVVEFALATGLRRSN
                     IINLEWQQIDMQRRVAWINPEESKSNRAIGVALNDTACRVLKKQIGNHHRWVFVYKES
                     CTKPDGTKAPTVRKMRYDANTAWKAALRRAGIDDFRFHDLRHTWASWLVQAGVPLSVL
                     QEMGGWESIEMVRRYAHLAPNHLTEHARQIDSILNPSVPNLSQSKNKEGTNDV"
     gene            complement(15302..15377)
                     /locus_tag="OO544_001615"
     tRNA            complement(15302..15377)
                     /locus_tag="OO544_001615"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15342..15344),aa:Thr,seq:cgt)
     gene            complement(15492..16745)
                     /gene="proA"
                     /locus_tag="OO544_001616"
     CDS             complement(15492..16745)
                     /gene="proA"
                     /locus_tag="OO544_001616"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HCR6767237.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESVEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALAQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            complement(16757..17860)
                     /gene="proB"
                     /locus_tag="OO544_001617"
     CDS             complement(16757..17860)
                     /gene="proB"
                     /locus_tag="OO544_001617"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HCR6767238.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            18148..19197
                     /gene="phoE"
                     /locus_tag="OO544_001618"
     CDS             18148..19197
                     /gene="phoE"
                     /locus_tag="OO544_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HCR6767239.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVMHYMSD
                     NASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGLKY
                     KDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFFGV
                     IDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQNLQ
                     SRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQFDF
                     GLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNKLN
                     INNDDIVAVGMTYQF"
     gene            complement(19236..19637)
                     /gene="crl"
                     /locus_tag="OO544_001619"
     CDS             complement(19236..19637)
                     /gene="crl"
                     /locus_tag="OO544_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HCR6767240.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(19695..20939)
                     /gene="frsA"
                     /locus_tag="OO544_001620"
     CDS             complement(19695..20939)
                     /gene="frsA"
                     /locus_tag="OO544_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HCR6767241.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     IQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            complement(21031..21489)
                     /gene="gpt"
                     /locus_tag="OO544_001621"
     CDS             complement(21031..21489)
                     /gene="gpt"
                     /locus_tag="OO544_001621"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCR6767242.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            21750..23207
                     /gene="pepD"
                     /locus_tag="OO544_001622"
     CDS             21750..23207
                     /gene="pepD"
                     /locus_tag="OO544_001622"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HCR6767243.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKSVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            complement(23264..23878)
                     /locus_tag="OO544_001623"
     CDS             complement(23264..23878)
                     /locus_tag="OO544_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HCR6767244.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALERLIKEAARQDVAVTVLETET
                     GRYSDTLRSALVSLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(23875..24485)
                     /locus_tag="OO544_001624"
                     /pseudo
     CDS             complement(23875..24485)
                     /locus_tag="OO544_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
BASE COUNT         6379 a         6218 c         5788 g         6100 t
ORIGIN      
        1 tgttctgaaa tttccagcac caacgttgta aacgaacgag taaagagcgc cgcgcgttgt
       61 ttccggtata tcgactttga tgtacgggtt aatttgtctg gcgaccgtgg caaggtcttt
      121 attcaggaga gctttgcatt ctgcttcggt atacgtttta ccgagcatga tgtcttttcc
      181 agtgtgtccg taacatacag tccatacgcc aacaatatct ttgtatggta tgtaactgac
      241 accttccaga ccatcgttac cactcggacc agtgatgagc acagacgcta tggcaacagc
      301 cccaccacca atagcagcag caacagcctt gcgtaatgat ggcgacatta ttcacctctc
      361 gcagccttac gcttatcttc tttaatcttg aaataaaggt ttgtcaggta cgtcagcagg
      421 ccaaatacca gactacccag cacacctatt gccgcccact gtgatggcgt gactttatcg
      481 agcaactgta aaaaccagta gccagcactg cctgcagagg tgccataggc gacacctgtt
      541 gttaacttat ccattgattt cataacccca cctcgcagat gcgggtgctg tgtaatggaa
      601 ataaaaaggc cacctgacgt ggccaccaga ttatttcccc accagctcgt ttatctcttt
      661 caccgtctgg ttaaaccgct ctgactcaag ctcaacacct aaggcccgac gccccagcgc
      721 cattgctgct tttattgtgg aaccggatcc cataaagaaa tcagcaacca gatcgcctgg
      781 tcgactactg gcattgatta tttgccggag catatccgca ggcttctcac acggatgttt
      841 acccgggtag aactgaacgg gtttatgcgt ccagacgtcg gtataaggca cggaaactga
      901 tacggagaaa tagcgccgga gagttttaaa ctcatccagc aattcagaat atttgcgatt
      961 cagtgaatca taagatgcca ccagctggtg gtgtggttgt tccagttgtt gttccagttg
     1021 ttgttcctga aacttctctg ccgctatacg ggaaaacagt gcctgcaact tccggtagtc
     1081 agcctcattc ggcaactgcc actgactggc accaaaccag tgggaaacca tgtttttctt
     1141 accagtggct tcggcaatct gttttgccgt tatacccagt tcggcacgag catccctgaa
     1201 atacgaaatc agcggtgcca ttatgtgctg tttgagttga ctttcttttg ccgcatagcc
     1261 gtcacttttg ccgcgatatg gcccctggta atgttcagca aacagaacgc gttctgttgc
     1321 gggaaaatat gcgcgcagac tttctttatt acacccgttc catcgtccgg acggcttcgc
     1381 ccagataata tggttaagaa cgttgaaacg ttcacgcatc atgatctcga tatcagatgc
     1441 gaggcgatgt ccacagaaca ggtaaaggct tccggcaggt ttcagcaccc gccagaactg
     1501 agccagacag tggtccagcc acttcaggta atcttcgtcc cctttccact gattgtccca
     1561 gccgttgggt ttcaccttga agtacggcgg atcggtaaca atcaggtcaa tggaatcatc
     1621 aggcagggac tgaataaaat gcaggcaatc agcgttgatt aaatcaacac tgtttatttt
     1681 tacagtattt ttcatggatc agtaagcgta actctggtag gctcactctg cttttgcgct
     1741 aaagcagtgg gccgtggttc gcttgtgacc agtaagcatg agcgaatggc tggcaggtgc
     1801 taccaacacc caccagccgc ccattttcac aaattaaaag cccttcattg ctgaaggcgt
     1861 ctgtaacagc cgaactggta atctgccagc cccgccataa ccaactgggt cagtattaac
     1921 tgacagcgtt cgcgtgaaag gtatgtgttt tgtgcaatct ccccgactgt tgccggttcg
     1981 atgcttaatt cattaaaaac aactttcgcc gtttctgtca tatcttgctg ttttagcatg
     2041 tctttttacc ttcatggtta acatgacata ccaataactc ttgtctaaaa agccagcaag
     2101 ataaaaaatc agtattcact accaccagcg tgtttaccgt actgcaccag gtttacaggt
     2161 acaaaaaaac ccgctcgaca gcgggtttaa gctgtgtggc gaagtaacca ctcttaacag
     2221 attacaagaa tttttgcgga ccgcgttaat gatttttaat cccaaagtcg tattattcgt
     2281 tttttactgt aggagttaat aggtttatat tatgttagat acactatctt tcactgaacg
     2341 tgacgagttc caacgaagaa acatcgctga aaatatcatc aagttgctaa aaccagaggc
     2401 agacatttca ccactggtaa tagacggcgc atggggaaca gggaaaacag aatttagtat
     2461 caaactgaaa aatctcatta ttgagcaaga aactgaatct aaagttgttt atattgatgc
     2521 ctttaaaggg gatcatgcag aatctccatt acttctcata acctctgcaa ttgccagcat
     2581 tttgcctgag gaagaaaaac aacacttcat taagagatct cttcctgcaa ttcgctttgg
     2641 tttaaaaacg gtactaaaag ctggtgcagg ttggttttta cgacaggagg ctagtgaagt
     2701 tgccgaagaa ttccaagatg cgatgaagaa agcaagcaat gcagcaatag atgggactat
     2761 tgaaaatttg cttgaagacc atatggactc agagaaaaac ataaattcac ttaaatcctg
     2821 catcgaaagc atatcaaaaa atcaaaaaat cgtaattata attgatgaat tagaccgatg
     2881 taagccgagt ttttcaacaa atgtcattga aacaataaaa cacatttttg acatcaataa
     2941 tgtctttttt attttagtta caaacacaga acaattaaaa gcatctataa atcatattta
     3001 tggttatagc attaactcac aaaagtatct tgacaagttt ataaaatata ccatcacgct
     3061 tccagataca tgtttgataa atggtcacaa cgtatgtaaa gcctctgtta tatattggga
     3121 ttaccttgta gaaaaaacaa ggttattgaa taaaatcaat agattatcag gttactttat
     3181 ccgtgattta attcaacgaa ccaacttatc attacgtgaa acacaaacat tttcacgcaa
     3241 tcttaacatt ttccaactgt taaacgacga tgagaacaaa agcagtgatc ctttaataaa
     3301 tatgatcttt gtcgtagctg ccttcataca ttgctttggt aacaaggaaa aactaaaaca
     3361 agaaattacc gctgaatcta tatcttattt agcagacctg cttaacataa aagaaatacc
     3421 ttattcttat gagagaagat cgcaaatccc tgaaatatcg attgtattct tcggaataat
     3481 taaagacagt attactctta atgagcgatt tgcccctaaa agtgatgaag aacttaaaaa
     3541 attcacaaat gtttatactg attatgaaca cataaatttt tggagtacta cacccagaga
     3601 gttattgata aaatatatta atcaaatgtc attcatccag taaataatac gccccatgca
     3661 ggggcgtatt attaaacgtc caaatctaat gttagatcta gcatggaaag gcagccatca
     3721 ataaatcctt cggctagctg tatttctata cgtatcaatt tctcatcttt tttacgagcc
     3781 ttggcgagct ttcttttaga gataccgtat aggtaatggg caacaagaag cgaatgttcg
     3841 tccggccttt tttgctttag acgagcaaga caaccttcaa taattaaggc atcactatcc
     3901 gaacaagcca agcgtttctt gcttgtatag ggaagaagtc ccttaaaccc agcggctata
     3961 ggtgaataat caacgcctga actatcactc gccgcccatg ccccccaacg ctccagaacc
     4021 atctgaatat cacgcatcaa ctttctccac caaatcaggc tagcacacca attgccaaca
     4081 cacggtcgat aaaacgaaat atcagctcca gctgggagcc atacttctct tcaaatgcca
     4141 cggtatccgc atgcagctcg tcgtgatgct ttctgcacaa aggcaacaca aagaggtcat
     4201 gcgcttttgt actcattccc ccctgaccgt ggcctatcag gtggtgggga tcatcagcag
     4261 gctttccaca acatgcacac ggctgtgtct taacccagcg cgtgtacttt tcattaaccc
     4321 agcggcgacg tttggggcgt aacataaaag actccggcga ctccggttcc actttcagcg
     4381 ccagcacctt tttcgcttta tcctgcatga tgctggtggc tggaaccgaa ggcacaaggt
     4441 cactttcccg ggtgacagac ggcacaacag gcttcggtaa tctcagtgcc ttacgggctg
     4501 cgctttccgg taaggcatcc gccagatcat tacgaaccag ccaccagcac agttccggca
     4561 ttgtcacaac gtgactatca tcaaaaccga gatcccgacg cacaacagac aacacccagc
     4621 gggcacagtt atccgttgcc attgattcca gccgttccgt gaactgatcg cgcagttggt
     4681 tatcgcagtg ccagcacaga cggattgcgc caggcgcgtg tcgcattgtg gtcatgttct
     4741 cgctgtgcca gttggaatga ggccactggc agcctttttc acgaagtaac cagctttcaa
     4801 gacattccac gccaccagca cgacggatca ctgcctcatt gcggaacacg gcccgaacgg
     4861 caggatcatc cgccagcggt tgtgatgccg ccggaacggc accactggcg aaagatgaat
     4921 aacgttccgg ctcaggctcc agcagtacac gcccctgcat aaacaggggc atcaactctg
     4981 aacctggcct gaacaatacg atccccatac gcggggcaat ttcaggggtc agtagtgctc
     5041 tcacggtcac ctcaatgaac ggtatcgagc agctttaaca gctcagggaa tcgggattcg
     5101 aagaaatgcg gctgcgtctc gcgcggattt gcaggactgg tgatgttctt gccgaacatg
     5161 cagcctttcg ccgtcagcga ccagaatttt ttgatgttgt taatcgctgt acggctgtat
     5221 cgttcgcgct gttcgacgat ccccagcttc gccatctggt gatatgcctg attagccgta
     5281 aggcggatac catactgttt cagcagtgca ctcagcgaca gtgtcgggcg acttgagcca
     5341 tcaggcgcgt cagcaggagc atcaatggca tagcgcggtg ccagattcgg taagccaaca
     5401 gcctcctgga gtttctgaca ggccccaagc actgaagagt tagacaggtt taactccctg
     5461 cgcataaagt ccagcagaat cacgccagcc tgcatcttgt cagcagcctg cccggataat
     5521 ttttccggtg cgctggttac catatcgaaa gtacggatca cctttagatg gaatgacggg
     5581 ctgatccaca ttgcataggc atacaccagt tctttgcaga catacgttcc ccgttcattt
     5641 cccccatgaa tcacactcac cgggtcaaca cccaaattct gggtgttggt caattcatga
     5701 acaagttcaa cagtttgttg gctggaaaga aactttcctg gctccttggt tctggcattt
     5761 gcaccagatg ctactgctgc gcgatgcaga tcgttcaggc tgtaacgtcc ataagcatca
     5821 cgacgaactt caataccatc aatgaccatc agattattca tacttcgttt ctcctcttga
     5881 tcaggcggct gcacccgccg ttttctcgta cttactgata gtgatctcga ccttcccttc
     5941 cgggataacc ggtccccact ccaccagcat tcttttcacc tgactgtcgt cttcccacac
     6001 ccccgcgtgg gtcagggcgt caaacagcgc cttgttatag ttgtccagat cgcggatcct
     6061 gttatccgga ggaaacaaca cgatctccac tgaagcaggt gccgacgttg gtttcggcag
     6121 acgacgtaac tgttcaacta ttgctgcgca cgccgcgctc tgaaattttc gccccgccgc
     6181 gcttatcagg ctcttacctg caaacgcccc tttgttgggg tgtcgccagt acgtgttcac
     6241 gctgggcggg aaaggcagga tcagcttcat actttcaggc ccctcttatg taaccagtgg
     6301 gttgcacgca gcctggcgtt ttcctcaccg gcaagcagtg agcggataat cccgaccgcc
     6361 tcgctgtcgt cgtccttcac cgcggtatga agcgtgatgc cccgggccac gccacgcttt
     6421 atcgtgatga cgcctttttt ctccagtgcg cgaagatgct ccaccgctgc attcactgaa
     6481 cggtatccca gcatggttgc cacctcctga ttggttggcg ggaagccacg ttctttctga
     6541 taagaaatca gcatatccag cacctgctgc tggcattgag ttaacgtcgt catgccgcca
     6601 tctccctgac cagtttttcc gcctgctggc gaacctgcgc cagaaaggcc tcaccacatg
     6661 cctcaagttc atcgcgcccg atatagctga ttgccgatcc cttccaggtc ttatcgaaaa
     6721 cagcaatagc accagcgaag aaagcgcctg tcggcacctg cttctcatcc ttcgggataa
     6781 accagacagg cagttcaaaa ccaatacgcc cgcgaataaa agcaatatgg tctgcatctt
     6841 ccggccacca cacttcgctg gtggcagctt tgatcaggaa aacataacgc ccgcccttat
     6901 cacgcatggc actggcatgt ttcatgatgt aacgcatgcc ggtgatgtat tgcccctcat
     6961 gctgactggc gcggctgtat gggggattac caaaggcagc acctttaagc tccgcaagac
     7021 gttctgacca gtcatgcgcc agcgcgttgt cttccgccgt gtaatacgca gcacatttgg
     7081 tgttatcacc gtcagtgaac agatccagaa caaacgggcc aaacagggtg ttaattcccc
     7141 agaaaatgtt gtccggcgtg cgccactgat cgcccacttc cttcagttca tgggctggtt
     7201 tgttccgcag ttccaccagc gcctggcaat atttattact cattaagccc ccacgtaatt
     7261 ccctgacaga taccactctt cacccgatgc agcgcgcttg ctgcttttcc gtaagcaccg
     7321 ctcacgacgc accagaaaat tgtttcgttc tggctgggag tggctttcac ggaatgccgc
     7381 catccacacg gttgcagcac gacggtataa gcccctggac tccagttctt ccgcctggcg
     7441 ggtcaggcac aaaatcaccc gcgggtcgtt agtgccgaca tagaaattgc gcacaggtct
     7501 ggtttcacga actggttgtg gttccggatc ctgcgctctc tcagtcaggc gcgggaaatg
     7561 tctgtgtgta tctccttcac aacggtgagc cacacgccca ctctgacgta acttgcttgc
     7621 tgactgcaga acgcgctgcc gtgagtaacc tgcaaaagca tccgcaatgt ctccggaagt
     7681 acagcccgga tgggcttcaa tgaatttctg aacgtcattc aaaagactca tgctcacccc
     7741 ctgaatcctg ccgggatctg gctgtagtcc acattgtcgt aactggcttt gaagtacggg
     7801 tcttcgcgtt tttctgtgta cgtgctgacg gacggcgata agcgcagaga aagctcatcc
     7861 catttttccc gcagcttcga cgggctgagc acgttacggc accagaacgg atcgcggctg
     7921 acgcggctgt acatctcgca gatttgtttg tgagtacgac catcctgcac acacatcagg
     7981 cgaatttcgt ttgcccaggc tgtccagttc ggttctttgg gacgaaccac ctcgccgtca
     8041 cattcggcgg cctgctcgta cagggcgatg atttttttcc agagccactg tgcgcaggtc
     8101 aaatcatcct gcgtccccca ctggcgcttt ttagggctga atacaaccgc atcaggatgg
     8161 cgagttaaaa aatactgttc agccgtctgc gtgtccggtt gcgaagcatc cggacgagaa
     8221 ggttttttat ctgacggatc atgttttgat tttactgacg gatccccgcc agattctgac
     8281 gggtgaaaac ccgctttttt gccagatttc gacgcatcaa attttgacgg gtcagatttt
     8341 gatgcgtcag attttgacgg gtcagaatct gacagttgag aaaatgccgc tgcctgaagc
     8401 ttcgcaacgt taagctgata aacattcgac gcattgcggt taccctggcg acgcgcctta
     8461 cgcgttaacc agccttctgc ttccagccgt gcgatagccg ttctgacggt actcatcccc
     8521 gcgccaatct ggcgggcaat ggtttcaatt gatggccagc acacaccttc gtcattactg
     8581 aaatcagcca ggcgggccat aattgccacg ctggataatt tcatgcctga cgctgcgcaa
     8641 ccatcccata catagccggt taatttagtg ctcatgaccg acctctattt ccctgaattt
     8701 acgacgaaac tgttcgagcg gactgaagca ctcatgctca tagccttcgc ggaggtagat
     8761 aacccgttgt gtttccggct cccaacgaat gactctgacg ggcactccgt agtgatcttt
     8821 gaaccagcgg ttaacttgtc gcaaaggact gtctccttct gccggttgaa atcccccaca
     8881 gcccactcag caaagctgtg ggttacaatt tccctgtcac ctggtacatt tactgcatag
     8941 caatactcca ccttcgcttt tccacccggt acaggaagcg caatcagttg cgagcgacgg
     9001 tagtgtgttg ttaaactgtt catgcgttag tttctccaca accagaagca atcgacgcca
     9061 cgacgcccgg agctgcacac tcgcgggcgt cattactttc tgaaacgcaa aaaattttgt
     9121 agacaagtgc tgcatgctcc tgcagcttcg aaattgagag gtacagctcg tcgttaattg
     9181 ctgtcttctc atgcggttcc actacaccgt cttcgattgc cgaacgaatc tgttttgaat
     9241 aactgccgat ctgttcaatg acttccagta aacgctggtt aatatcggca ttgtccacat
     9301 cctcgacgtc aggaagagac acaaagacgc catttgcaga ctgcgccaca gcgtcggcaa
     9361 tgaagtgagt gccaccagca cgttgtaaaa tcattgccca tcccagcggg aaaatctgat
     9421 cgccatcggc acgaaggcgg ttaaataatg cgttctctgt tacatccagc cactcagcag
     9481 cttcagcgta accccccggc aacgccgcga tagtttttct gacagctttc acgtaccact
     9541 caggctgttt ttccactttc cagtgatgat tacccacggc ttacctcctg ttcctgtggt
     9601 ttaaacccat tctggttttg gctagattga aaacgtgccg gataaagaat ctgcatttcg
     9661 ctgatttcac ccttaaaaaa attggccaga cgttctgcaa gatcgataga tggaatttgt
     9721 tccagtcttt caatacgact cagcgtcgct ggattgacct gaacgccagc agcaacatgc
     9781 tgcaaagtaa atccgtgcgc cttacgcaca ttccgtaatg gtgattgcat atgacctcca
     9841 catattgcgt gatgagcata ttatttcacg caaatatttt gcgcaagttg atttgcttaa
     9901 cgcgcaataa agaaatgtaa taaacgcatg aacataggaa accgagtcag acaacttcgc
     9961 caggcgaaga acatgaaaat cgccgatctc gctgaagcaa taggagtgga tgcggcgaat
    10021 atctcacgcc tggaaacagg taagcagaaa caattcactg aacaagccct gagtaatatt
    10081 gccaggagct taggtgttga tattgctgat ctctttacct cagacgtcaa aagtaatact
    10141 gtatgtaaaa acagtattag tgaggatgtt gcgcaggtga aggatgtatt ccgtattgaa
    10201 atgctggatg tcagtgccag tgcgggaaat ggccttatcc agggcggtga tgtcattgat
    10261 gtgattcatg ccattgaata cagaactgat aatgctgtat cgatgtttgg cggacggcca
    10321 gccaatcaca ttaaagttat caacgttcgt ggggacagta tgtgtccaac cattgagcca
    10381 ggagatctca tcttcgttga tgtcagtatc aatcagtttg atggagatgg tatctatgta
    10441 tttggttttg atgataaaat ttatgtcaaa cgactgcaaa tgatacctga caaactactg
    10501 gtgatttctg ataaccagat ttaccgtgaa tggggaatta ccagcgaaaa tgaacaccgg
    10561 tttatggtct ttggaaaggt cttaatcagc cagtcacaaa cccttaagcg acacaattaa
    10621 cccttacctc ctcatcaatt agccacccaa aggtggcttt tcattaccct ttaaattgca
    10681 tatctcgcaa caaaaacact tgcataatgc gcaacttcat tttatctttc tttccagaca
    10741 aacaaacaag gtactaacaa aatttggttg taacacggcg tatggcacat gcgtcgttag
    10801 cggtctgggg acgttaaagg ggacaatcca ctccttgctc gggcaaacaa accaggtagc
    10861 cggaatgtgc aagtcaatga tgatgctgat aagacgccta accagcgtgg cgattcggtt
    10921 tgacgcctgg gaagagacca gggtgcaacg atgagggcat ttatggagcc gcgacaaagt
    10981 gtggtgccgt aactggctaa gtgctctcag cgttgtggta atccgcgaaa tggcgcggcg
    11041 gtaagtatgg cggggttact ctttccccgt tgaggacacc ggattgtcag gttgaccata
    11101 cgcctgagtg acaaccccac cacaacagcc actgctttgg cggtaccagt ttgtaccctt
    11161 gcttccggct ggtaccgctc tttttacaaa acagagaaga gcatcaccgg acgacgggct
    11221 cataacccaa tccatccggg cggcagtcac cgcaggtgtt cttctctgtt ttgtggagaa
    11281 accaaccgac cttgcagggt cgatatgatg aggagcagca aaatggctag cgaacgcagt
    11341 actgatgtgc aggcatttat cggggagctg gacggcggcg tatttgaaac caaaatcggc
    11401 gctgttctca gtgaagtcgc ttccggtgtg atgaacacga aaaccaaagg taaggtctcg
    11461 ctcaacctgg aaatcgaacc gtttgatgag aaccgtgtga aaatcaaaca caaactctca
    11521 tatgttcgcc cgactaaccg cgggaaaatt tctgaagaag acaccaccga aacgccgatg
    11581 tatgtcaatc gcggtggtcg cctgactatt ctgcaggaag accagggaca attactgact
    11641 cttgccggtg aacctgacgg aaaacttcgc gcagcaggtc attaatatcg ttcttaatta
    11701 actgattatt tatctcatca ctgaatatct ttatatagtg aggacttatt atgtctcaga
    11761 acttagacgc aaccgcaatt aatcaaatcc atgctcttat ttctgctcag ggtgttaatg
    11821 aaattatcag taagattggt gccgatgctg tggcattgcc tgagaatttc cgcattcatg
    11881 atctggaaaa atttaattta aatcgcttcc gtttccgtgg tgcgctttcc actgccagca
    11941 tcgatgactt tacccgttat tctaaagatc ttgcagatga aggcacccgc tgctttatcg
    12001 atgccgataa tatgcgagcc gtcagtgtgc ttaacctggg tactattgat gaaccaggtc
    12061 acgcagataa caccgccact ctcaaactga aaaagacagc accgttctct gctctgttgt
    12121 ctgttaatgg cgagcgtcat tcccagaagt cactggcaga atggattgaa gactgggccg
    12181 actaccttgt gggctttgat gctaatggtg acgctattca ggcaacaaaa gcggctgcgg
    12241 ctgtccgtaa aatcacgatt gaagcaaacc agaccgctga ttttgaagat aatgacttca
    12301 gcggcaaacg ctccctgatg gagtctgtcg aagcgaaaac caaagatatt atgccagtgg
    12361 catttgaatt taaatgcgtt ccgtttgaag gtctgaaaga acgtccattt aaattacgcc
    12421 tcagcattat cactggcgat cgtcctgtac tggttctgcg cattattcag ctggaggcgg
    12481 tgcaggaaga aatggctaac gaatttcgtg atctgcttgt tgagaaattc aaagacagca
    12541 aagtagaaac ctttattggt actttcaccg cctgatttca ttactgcaaa tgcccctgcg
    12601 ggggcattta tggaaacgta attgactcaa taatcgccgg atggtgaggg cttcctttta
    12661 ccagaattca gcgtggtgca gcacatatac gcggagaaca aaatgtcatt tattaaaact
    12721 ttttccggga agcattttta ttatgacagg ataaataaag acgacatcgt gattaacgat
    12781 atcgcggttt ccctttcaaa tatctgtcgc tttgccggtc atctttcaca cttctacagt
    12841 gtcgcccaac atgcggtgct ttgcagccag ctggtgccgc aggaatttgc ttttgaagcg
    12901 ttaatgcatg atgcaacaga agcgtattgc caggacatcc ccgcgccgct gaaacgcctt
    12961 cttcctgact ataaacggat ggaagaaaaa atagacgccg taatccgtga gaaatacggg
    13021 ttacccacgg ttatgagcac gcctgtgaaa tatgccgatc tcatcatgct ggcaaccgaa
    13081 cgccgcgatc tcgggcttga tgatggctct ttctggccag tactggaagg tatcccggca
    13141 acagagatgt tcaaagttat tccactggct ccgggccatg cctacgggat gtttatggaa
    13201 cgttttaacg agttatcgga gttacgcaaa tgcgcatgaa tgttttcgaa atggaagggt
    13261 ttcttcgcgg gagatgtgta ccacgagacc tgaaagtgaa tgaaacggat gctgaatacc
    13321 tagtacgtaa attcgatgcg cttgaagcta aatgtgcagc actggaaaac aaagtaatac
    13381 cagtgtcaac tgaactgcca ccagcaaatg aaagtgttct gttatttgat gctaacggag
    13441 aaggctggct gattggctgg cgttctctct ggtacacctg gggacaaaaa gaaaccggag
    13501 aatggcagtg gacatttcag gtcggggacc ttgaaaacgt caatatcact cactgggcag
    13561 taatgccaaa agcaccggag gctggagcat aatgaccact tttaccgaca aagaactgat
    13621 taaagaaatt aaagagcgta tcagcagcct tgacgtgcga gacgatattg agcgccgtgc
    13681 ttatgaaatc gcactcctat ctctggaagt agaaccagat gaacgcgaat cttatgaatt
    13741 attcatggaa aagcgtttcg gtgacttagt agatcgtcgg agagcaaaaa acggcgataa
    13801 cgaatacatg gcatgggata tgactctcgg ttggatcgtc tggcagcaac gagctggtat
    13861 ccatttttca acaatgtcac agcaagaggt gaaataatgg agccatacag cctcacactc
    13921 gatgaggcct gtcattttct caaaatatcc agaccgactg ccattaactg gatacgcaca
    13981 gggcgtcttc aggcaacacg caaagatccc actaagaata aatctcctta cctcacaact
    14041 cgacaagcct gcattgcggc tcttcagtct ccgctgcata ctgtccaggt gagcgcgggt
    14101 gatggcataa cagaggaaag aaaatgtcac tcttccgcag aggtgaaata tggtacgcca
    14161 gtttcacatt gccgaacggt aaaagattta aacagtctct tggaacaaag gacaaaaggc
    14221 aggcgacaga actccatgac aagctaaagg ctgaagcatg gcgggtcagc aaacttggtg
    14281 aaatacctga tataacgttc gaggaagcgt gtgtcaggtg gcttgaagag aaagcacata
    14341 aaaaatcact ggacgatgac aaaagccgga tcggattctg gcttcaacat ttcgcaggaa
    14401 tgcaactaag agacattact gaatcaaaaa tttattcagc aatgcagaaa atgacgaacc
    14461 ggcgtcatga ggaaaactgg aaactcaggg cagaagcatg cagaaaaaaa gggaaacctg
    14521 ttccagaata cacgccaaaa ccagcgtccg ttgcaacgaa ggctacgcat ctttcattta
    14581 taaaggccct actaagagcc gcagagcgtg aatggaaaat gctggataag gcaccaatta
    14641 ttaaagtgcc tcaaccaaag aataaacgga tccgctggct ggagccccat gaagcacaaa
    14701 ggctgattga tgaatgtccg gagccattaa agtctgttgt tgaatttgca ctggcaacag
    14761 gtttaagacg ctcgaacatc atcaaccttg aatggcaaca aatagatatg cagcgccggg
    14821 tggcatggat aaacccggaa gagagtaaat caaaccgcgc aatcggcgtt gcgctgaatg
    14881 atactgcatg tcgcgttttg aaaaaacaaa tcgggaatca tcaccgttgg gtatttgtgt
    14941 acaaggaaag ctgtaccaaa ccagacggaa cgaaagcgcc aacagtaagg aagatgcggt
    15001 atgacgcaaa cacagcctgg aaagcggcgc tgagacgagc tggtattgat gatttcagat
    15061 ttcacgactt gagacacacc tgggcaagtt ggctggttca agccggagtc ccgttgtcag
    15121 tgttacagga aatgggtggc tgggagtcta tcgaaatggt tcgtcgatat gctcacctcg
    15181 cgcctaatca ccttaccgaa cacgcacggc aaatagactc gattctgaac ccatcggtcc
    15241 caaatttgtc ccagtcaaaa aataaggaag gtactaatga tgtgtaactt attgatttaa
    15301 atggtgccga taataggagt cgaacctacg accttcgcat tacgaatgcg ctgctctacc
    15361 aactgagcta tatcggccct gaaaggacat gttcacgaac gtgaatcacg gtggacaagg
    15421 ttaaaactaa ccgggcgatg cgtcaatggc cttatgaatc aaatggctac ttttgcatca
    15481 cccggtttta tttacgcacg aatggtgtaa tcaccaatgc cgatccactt gtaagtggtc
    15541 agtgcttcca gccccattgg gccacgcgcg tggagttttt gtgtgcttac cgccacttcc
    15601 gcacccagac caaactggcc gccgtcggta aaacgcgtag aggcgttaac gtaaacagcg
    15661 gacgaatcca cttcgttaac aaaacgctgg gcgttgcgca tatcgcgggt caggatcgca
    15721 tcggagtgtt gtgtgccgtg ttcacgaata tgggcgatgg catcgtcaag atcgctgacg
    15781 attttgacgt tcaaatctaa tgacagaaac tcatcgtcat actcttcggc tttaacagca
    15841 accaccttcg cagggcctgc ctgcaactgc gccagtgcag ctgcatctgc gtgtaatgtc
    15901 acgccgcttt ccgccatttg tttgcttaat gcgggcagga agctatcggc gatgttttta
    15961 ttcaccagca acgtttcaac cgtattacat gtgctcggac gctgagtttt cgcgttgacg
    16021 atcactttta atgcttcagc gatctctaca ctttcatcaa cgtaaatatg gcatacgcct
    16081 ataccacctg tgatcaccgg gattgtcgac tgttcacggc acagtttatg caaaccagcg
    16141 ccaccacgcg ggatcagcat gtcgatgtat ttatccatac gcagcatttc actgaccagc
    16201 gcacggtcag gattatcaat cgcctgcacg gcacccgccg gtaagccgca ggatttcagg
    16261 gcgtcctgaa tcaccgccac cgttgcagcg ttagtgcgac acgtttcttt gccaccgcgc
    16321 aggatcaccg cattaccggt tttcaggcac agcgaagcga catcaaccgt cacgttcggg
    16381 cgcgcttcat aaatcacgcc aataaccccc agcggtacgc gacgacgctc aagacgcagg
    16441 ccgctgtcca gtacgccgcc atcgattacc tgccccaccg gatcggcgag gttgcacacc
    16501 tgacgtacat cgtcggcaat gcctttcagc cgtgcgggcg tcagtgccag acggtcaagc
    16561 atcgcttcgc taaggccatt ggctcgcgcg tcagcaacat cctgggcgtt agcgttgagg
    16621 atgatttcgc tttgtgcttc cagttcatcg gcgatttttt ccagcacgcg atttttttcg
    16681 cggctggaga gttgcgctaa tttatacgag gcttgcttcg cagcaatgcc catttgttcc
    16741 agcatcggcc agctccttaa cgggtaatca tgtcatcacg gtgaacggca accgggccgt
    16801 attcatatcc cagtattgca tcaatttctt gcgagtggtg tccggcaata cggcgtaatg
    16861 catcgctgtt gtaacgactg acgccgtggg cgatatcgcg gccttcgagg ttgcaaatgc
    16921 ggatgacttc accacgcgag aaattgccag tcacgctttt aatgcctttc ggcaacaggg
    16981 agctgccgcg ttccagaatg gcggcagttg ccccttcatc taccgtgatt tcacccgccg
    17041 gcggcgcacc gaaaatccag cgtttacggt tttcaagcgg agtcgcctgg gcatggaaca
    17101 gcgtaccgac ggaaatgcct tccatcacat caccaataac gcccggcttg ctgcccgcgg
    17161 caataatggt gtcgataccc gcacggcaag ccacgtcagc ggcctgcaat ttggtactca
    17221 tgccgccagt tccgaggcct gaaacgctgt caccggcaat cgcgcgcagt gcgtcatcaa
    17281 tgccgtaaac atctttaatc agttctgcct gcggattgct gcgcgggtca gcggtataca
    17341 aacctttttg atcggtcagc agcaacagtt tatcggcacc cgccagaatc gccgccagcg
    17401 cagaaaggtt atcgttatcg ccgactttaa tctctgccgt agcgacagca tcgttctcat
    17461 tgattaccgg aacgatattg ttatcgagca acgcacgcag ggtgtcgcgg gcgttcagga
    17521 agcgttcacg gtcttccata tcagcacggg tcagcagcat ttgcccgacg tgaatgccat
    17581 aaatcgaaaa cagctgttcc cacagttgaa tcagtcgact ctgccctacc gctgccagca
    17641 gttgtttcga ggcgatagtc gctggcagtt ccgggtaacc caggtgctca cgtccggcgg
    17701 cgatcgcgcc cgacgtcaca ataacaatcc gatgcccggc ggcatgtaac tgcgcgcact
    17761 ggcgaacaag ttcaacaata tgggcacggt tcagacggcg cgatccgcct gttagcacac
    17821 tggtgccgag ttttaccacc agcgtctggc tgtcactcat gattctctgc cattcaattt
    17881 taggaaaaat gatatcaaac gaacgtttta gcaggactgt cgtcggttgc caaccatctg
    17941 cgagcaaagc atggcgtttt gttgcgcggg atcagcaagc ctagcggcag ttgtttacgc
    18001 ttttattaca gatttaataa attaccacat tttaagaata ttattaatct gtaatatatc
    18061 tttaacaatc tcaggttaaa aactttcctg ttttcaacgg ggctctcccg ctgaatattc
    18121 gcgcgttaat taaaatcagg aatgaaaatg aaaaagagca ctctggcatt agtggtgatg
    18181 ggcattgtgg catctgcatc tgtacaggct gcagaaatat ataataaaga cggtaataaa
    18241 ctggatgtct atggcaaagt tatgcattat atgagtgata acgccagtaa agatggcgac
    18301 cagagttata tccgttttgg ttttaaaggt gaaacacaaa ttaacgatca actgactggt
    18361 tatggtcggt gggaagcgga gtttgccgga aataaagcgg agagtgatac tgcacagcag
    18421 aaaacgcgtc tcgcttttgc cgggttgaaa tataaagatt tgggttcttt cgattatggt
    18481 cgtaacctgg gcgcgttgta tgacgtggaa gcctggaccg atatgttccc ggaatttggt
    18541 ggcgattcct cggcgcagac cgacaacttt atgaccaaac gcgccagcgg tctggcgacg
    18601 tatcggaaca ccgacttctt cggcgttatc gatggcctga acttaaccct gcaatatcaa
    18661 gggaaaaacg aaaaccgcga cgttaaaaag caaaacggcg atggcttcgg cacgtcattg
    18721 acatatgact ttggcggcag cgatttcgcc attagtgggg cctataccaa ctcagatcgc
    18781 accaacgagc agaacctgca aagccgtggc acaggcaagc gtgcagaagc atgggcaaca
    18841 ggtctgaaat acgatgccaa taatatttat ctggcaactt tctattctga aacacgcaaa
    18901 atgacgccaa taactggcgg ctttgccaat aagacacaga actttgaagc ggtcgctcaa
    18961 taccagtttg actttggtct gcgtccatcg ctgggttatg tcttatcgaa agggaaagat
    19021 attgaaggta tcggtgatga agatctggtc aattatatcg acgtcggtgc tacgtattat
    19081 ttcaacaaaa atatgtcagc gtttgttgat tataaaatca accaactgga tagcgataac
    19141 aaattgaata ttaataatga tgatattgtc gcggttggca tgacctatca gttttaatgg
    19201 atattgccgg atgtgataca tccggcacat ttcactcacg ccgttaactt caccggttca
    19261 tcacgaaaat catccgccgg ttccagcttc agattcagcg tcgtcagcag ttcacgcagc
    19321 ttctcgtgaa actcacgtag ggtgtgctcc agtcgttcaa ccacttcagt gtcttttacc
    19381 ggaacactct tccagtcgcc tgctttatcg aacagaccaa actggtaact gtaggtaaaa
    19441 cgggattcct gcgcttcaag ctccatccac cagccccaga actcacgcac ttccggtgcc
    19501 ggtttcacgt tgacgcatac agccagacaa tcgaaaaaga atcgattatc ttcgcactta
    19561 ccttcacgaa tatacgggcc tagtgcggta aattttttga tcaatctgct cttcgggtgt
    19621 ccactcggta acgtcattgc aatctcctgt tgtgatgcaa ctgttttacc aaattggcaa
    19681 attttagcaa atttttaaca caagcgtttt tcgatccagc cggtgatttc ctgaagacct
    19741 ttgtcaaaat tccgatacac cgggttaaat gggatctcta ataatttacc gtcagcagat
    19801 gatgaggtga ttaagcgtga gtcctcttcc gggctgaacg gatcgttctt ccagtagcct
    19861 gataacattg gcgttgggca gcgacgcccc agcaatcctt gcacttttaa tgaatagcga
    19921 ttcagctcca cgcgtaacgc ttcgtcggaa gcatcatgca tccccaaacg actcgccaga
    19981 acgtcaagat acatttccgg cacctgttgc tggcacttaa aatcactcaa cagggtatga
    20041 actaccggac caagacaggc aaccgctttc agacgcggcg attcaagata tgccagacgc
    20101 acggcaacgt tagcgccgaa acggaaacca aaggccgcga cgcgagtgtg atccacccac
    20161 ggtacgttag gcagagcctt taaaacgtgc tgatgcaaca ggctggagtc ctgggtgagc
    20221 ttccattttg atgaaaagcc caccgacggc atatcaatag tcagcatcgc aatgccgcgc
    20281 ggcgcaaaat aacgttcata caggctgtaa tagtccgtct gcatcgcatc cagaccacca
    20341 cacattaata ctgtcgggaa cgggccatcg cctttcggca tatgcaaaaa gccggtgatg
    20401 ggcgcaccgc cgggtacggt aaactccatc tgccgcatcg tgcccggcag acgctgggcg
    20461 gcttcttcat aggcacggtt tgacaaagcc tgcgcttgct cggccaggtc atctcctttc
    20521 agatgaggat aggcagcaat gttgtacaac gtagccgcat gcagccagtg acgaccactg
    20581 agttgcggat cttcttccgc acaggctttt tgttgccaca ccatcgcctg gatggcccac
    20641 tcataaatcc agttgccacc acggtagcca atcaccgtat cgtataaatc atcgtcggta
    20701 cgttcggcat cgctcatcac aatacgcgcc tggacgtcga ggatttcacg tgggtcaatg
    20761 ccgcgccaga tccacatcag acggttaatc atgcgatacc agtgagggat ggttttacca
    20821 tcaagggccg actgcacagg cggttgtgcg ccgtgattaa agcggcggac tagcgtcgag
    20881 gtttcaggat gtttaaagcg gggtttgaac agggtttcgc tcaggtttgc ctgtgtcatg
    20941 gatgcagcct ccagaatact tactggaaac tattgtaacc cgcctgaagt taaaaagaac
    21001 aacgcccggc agtgccaggc gttgaaaaga ttagcgaccg gagattggcg ggacgaatac
    21061 gacgcccata tcccacggct gttcaatcca ggtatcttgc gggatatcaa caacatagtc
    21121 atcaaccagc ggacgaccag ccggttttgc gaagatggtg acaaagtgcg cttttggata
    21181 catttcacga atcgcaaccg cagtaccacc ggtatccacc aggtcatcaa taacgatgaa
    21241 gccttcgcca tcgccttctg cgcgttttag cactttaagc tcgcgctggt tgtcgtgatc
    21301 gtagctggaa atacaaacgg tatcgacatg acgaataccc agttcacgcg ccagtaacgc
    21361 acccggtacc agaccgccac ggcttacggc aataatgcct ttccattgtt cagaaggcat
    21421 cagtcggctt gcgagtttac gtgcatggat ctgcaacatg tcccaggtga cgatgtattt
    21481 ttcgctcatg tgaagtgtcc cagcctgttt atctacggct taaaaagtgt tcgaggggaa
    21541 aataggttgc gcgagattat agagatctgg cgcactaaaa accagtattt cacatgagtc
    21601 cgcgtctttt tacgcactgc ctctccctga cgcgggataa agtggtattc tcaaacatat
    21661 ctcgcaagcc tgtcttgtgt tgacaacatt ttctgctaac cctgtgacct gcaatactgt
    21721 tttgcgggtg atcgacaagg agacttaacg tgtctgaact gtctcaatta tctccacagc
    21781 cgctgtggga tatttttgcc aaaatctgtt ctattcctca cccgtcctat catgaagagc
    21841 aactcgctga atacattgtt ggttgggcaa aagagaaagg tttccatgtc gaacgcgatc
    21901 aggtaggtaa tatcctgatt cgtaaacctg ctaccgcagg tatggaaaat cgtaaatcgg
    21961 tcgtcttaca ggcccacctc gatatggtgc cgcagaaaaa taacgacacc gtgcatgact
    22021 tcacgaaaga tcctatccag ccttatattg atggcgaatg ggttaaagcg cgcggcacca
    22081 cgctgggtgc agataacggc attggtatgg cgtctgcgct ggcggttctg gctgacgaaa
    22141 acgtggttca cggcccgctg gaagtgctgc tgaccatgac cgaagaagcc ggtatggacg
    22201 gtgcgttcgg cttacagagc aactggttgc aggctgatat tctgattaac accgactccg
    22261 aagaagaagg tgaaatctac atgggttgtg cggggggtat cgacttcacc tccaacctgc
    22321 atttagatcg tgaagcggtt ccagctggtt ttgaaacctt caagttaacc ttaaaaggtc
    22381 tgaaaggggg tcactccggc ggggaaatcc acgttggcct gggtaatgcc aacaaactgc
    22441 tggtgcgctt cctagcgggt catgcagaag aactggatct gcgccttatc gatttcaacg
    22501 gcggcacact gcgtaacgcc atcccgcgtg aagcctttgc gaccattgct gtcgcagctg
    22561 ataaagtcga cgtcctgaaa tctctggtga atacctatca ggagatcctg aaaaacgagc
    22621 tggcagagaa agagaaaaat ctggccttgt tgctggactc tgtagcgaac gataaagctg
    22681 ccctgattgc gaaatctcgc gataccttta ttcgtctgct gaacgccacc ccgaacggtg
    22741 tgatccgcaa ctcagacgtg gcaaaaggtg tggtcgaaac ctccctgaac gtcggtgtgg
    22801 tgaccatgac tgataataac gtagaaattc actgtctgat ccgttctctg atcgacagcg
    22861 gtaaagacta cgtggtgagc atgctggatt cgctgggtaa actggctggc gcgaaaaccg
    22921 aagcgaaagg cgcatatcct ggctggcagc cggacgctaa ttctccggtg atgcatctgg
    22981 tacgtgaaac ctatcagcgt ctgttcaaca aaacgccgaa catccagatt atccacgcgg
    23041 gcctggaatg tggtctgttc aaaaaaccgt atccggaaat ggacatggtt tctatcgggc
    23101 caactatcac cggtccacac tctccggatg agcaagttca catcgaaagc gtaggtcatt
    23161 actggacact gctgactgaa ctgctgaaag aaattccggc gaagtaatta tttgatttgc
    23221 tgccggatgg cgtttaatcg ccttccggca gtttcatcct tcattatcct tcgataaaag
    23281 ccatccctgt aaatgtccgt ctcgggttgc cacgttcaat ctggtgatgg aacattcgcc
    23341 gctgcgattt cagcaccgcg ctattttcct gttgctgctg ctccagcttc caggcaatca
    23401 gcaatcgtgc cagccgcttg ttagcatgct gactacgctc tgactgaacc ttcacgctaa
    23461 taccggatgc caaatgcgtg gcgcgtaccg ccgagtcggt tttattgaca tgttgaccgc
    23521 ccggccccga cgaacgcaac gtctcataac ggattgcatc cgattgttcc tgctcatcag
    23581 cggtaaaacg cccaatgccc agaaaccagt ttttgcgccc atgatgaggc cgatacggac
    23641 tcggacaaat ccactgaata gtgccgcacc agctttcgct taatgcccat gcgttatcgc
    23701 catccagaga aaccagagct gaacgcagcg tgtcagagta gcggcccgtt tctgtttcca
    23761 acaccgttac cgcgacgtct tgtcgggcag cttctttaat taatcgctcc agcgcctttt
    23821 tcactgcgag acaacattct tccggcccct gcgctgagga gagttgtagc aagatcatcc
    23881 ttttttccct ccactgtttt tgagcgttag caccggacgc agccgcgcga caggaataat
    23941 taaaccagcc tgcaccagac attgcaccac gctttcagcc gatttatagg cttgtggcgc
    24001 ttcttcaaag atgagttgtt tatcgcgaca aattacccga ctgccaagtt cagtacgtga
    24061 gagctgcgtc gctgtgtatt tcgctgccag acgcccttta cactcggtgc gcccccattt
    24121 acgcccagcc ccatgcgcca gcgagtggag cgttttttcg ttcgcaacag gctgtacaag
    24181 ctaggagtaa tcaccgagtg aaccgggaat aatcaccaga ccgttgtcat ccggcgtggc
    24241 acctttacga tgcaaccagc cctgttgatc gccgatttga cacgcgctaa caaagttatg
    24301 cgccacatcc agaaccggac ttccggtggc cttaacctgt tgcattatgc gtaaagagat
    24361 cagctggcga ttaatacgcg caaacgccag cgcatcgtca tgttccgcaa tataccgtag
    24421 cgcgtcgtca ctgccttcag gcaaaccatg atgcgaaaac gaggcaatat gccgctgtaa
    24481 aatag
//
LOCUS       DAJTZL010000045        24278 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.045, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000045 DAJTZL010000000
VERSION     DAJTZL010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24278
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.045"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..200)
                     /locus_tag="OO544_001625"
     CDS             complement(<1..200)
                     /locus_tag="OO544_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator"
                     /protein_id="HCR6767245.1"
                     /translation="MRNFPVPYSNELIYSTIARAGVYQGIVSPKQLLDEVYGNRKVVA
                     TLGLPSHLGVIARHLHQTGRYAV"
     gene            complement(203..1870)
                     /locus_tag="OO544_001626"
     CDS             complement(203..1870)
                     /locus_tag="OO544_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR6767246.1"
                     /translation="MSATRIQAVYRDTGVEAYRDNPFIEALPPLQESVNSAASLKSSL
                     QLTSSDLQKSRVIRAHTICRIPDDYFQPLGTHLLLSERISVMIRGGYVGRNPKTGDLQ
                     KHLQNGYERVQTGELETFRFEEARSTAQSLLLIGCSGSGKTTSLHRILATYPQVIYHR
                     ELNVEQVVYLKIDCSHNGSLKEICLNFFRALDRALGSNYERRYGLKRHGIETMLALMS
                     QIANAHALGLLVIDEIQHLSRSRSGGSQEMLNFFVTMVNIIGVPVMLIGTPKAREIFE
                     ADLRSARRGAGFGAIFWDPIQQTQRGKPNQEWIAFTDNLWQLQLLQRKDALLSDEVRD
                     VWYELSQGVMDIVVKLFVLAQLRALALGNERITAGLLRQVYQDELKPVHPMLEALRSG
                     IPERIARYSDLVVPEIDKRLIQLQLDIAAIQEQTPEEKALQELDTEDQRHLYLMLKED
                     YDSSLLIPTIKKAFSQNPTMTRQKLLPLVLQWLMEGETVVSELEKPSKSKKVSAIKVV
                     KPSDWDSLPDTDLRYIYSQRQPEKTMHERLKGKGVIVDMASLFKQAG"
     gene            complement(1867..3975)
                     /locus_tag="OO544_001627"
     CDS             complement(1867..3975)
                     /locus_tag="OO544_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000267723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HCR6767247.1"
                     /translation="MWQINEVVLFDNDPYRILAIEDGQVVWMQISADKGVPQARAELL
                     LMQYLDEGRLVRTDDPYVHLDLEEPSVDSVSFQKREEDYRKILPIINSKDRFDPKVRS
                     ELVEHVVQEHKVTKATVYKLLRRYWQRGQTPNALIPDYKNSGAPGERRSATGTAKIGR
                     AREYGKGEGTKVTPEIERLFRLTIEKHLLNQKGTKTTVAYRRFVDLFAQYFPRIPQED
                     YPTLRQFRYFYDREYPKAQRLKSRVKAGVYKKDVRPLSSTATSQALGPGSRYEIDATI
                     ADIYLVDHHDRQKIIGRPTLYIVIDVFSRMITGFYIGFENPSYVVAMQAFVNACSDKT
                     AICAQHDIEISSSDWPCVGLPDVLLADRGELMSHQVEALVSSFNVRVESAPPRRGDAK
                     GIVESTFRTLQAEFKSFAPGIVEGSRIKSHGETDYRLDASLSVFEFTQIILRTILFRN
                     NHLVMDKYDRDADFPTDLPSIPVQLWQWGMQHRTGSLRAVEQEQLRVALLPRRKVSIS
                     SFGVNLWGLYYSGSEILREGWLQRSTDIARPQHLEAAYDPVLVDTIYLFPQVGSRVFW
                     RCNLTERSRQFKGLSFWEVWDIQAQEKHNKANAKQDELTKRRELEAFIQQTIQKANKL
                     TPSTTEPKSTRIKQIKTNKKEAVTSERKKRAEHLKPSSSGDEAKVIPFNAVEADDQED
                     YSLPTYVPELFQDPPEKDES"
     gene            complement(3962..4783)
                     /locus_tag="OO544_001628"
     CDS             complement(3962..4783)
                     /locus_tag="OO544_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heteromeric transposase endonuclease subunit
                     TnsA"
                     /protein_id="HCR6767248.1"
                     /translation="MAKANSSFSEVQIARRIKEGRGQGHGKDYIPWLTVQEVPSSGRS
                     HRIYSHKTGRVHHLLSDLELAVFLSLEWESSVLDIREQFPLLPSDTRQIAIDSGIKHP
                     VIRGVDQVMSTDFLVDCKDGPFEQFAIQVKPAAALQDERTLEKLELERRYWQQKQIPW
                     FIFTDKEINPVVKENIEWLYSVKTEEVSAELLAQLSPLAHILQEKGDENIINVCKQVD
                     IAYDLELGKTLSEIRALTANGFIKFNIYKSFRANKCADLCISQVVNMEELRYVAN"
     gene            complement(4942..6771)
                     /gene="glmS"
                     /locus_tag="OO544_001629"
     CDS             complement(4942..6771)
                     /gene="glmS"
                     /locus_tag="OO544_001629"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HCR6767249.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLDGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(6933..8303)
                     /gene="glmU"
                     /locus_tag="OO544_001630"
     CDS             complement(6933..8303)
                     /gene="glmU"
                     /locus_tag="OO544_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HCR6767250.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKVGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(8654..9073)
                     /gene="atpC"
                     /locus_tag="OO544_001631"
     CDS             complement(8654..9073)
                     /gene="atpC"
                     /locus_tag="OO544_001631"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HCR6767251.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(9094..10476)
                     /gene="atpD"
                     /locus_tag="OO544_001632"
     CDS             complement(9094..10476)
                     /gene="atpD"
                     /locus_tag="OO544_001632"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HCR6767252.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(10503..11366)
                     /gene="atpG"
                     /locus_tag="OO544_001633"
     CDS             complement(10503..11366)
                     /gene="atpG"
                     /locus_tag="OO544_001633"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HCR6767253.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKSVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(11417..12958)
                     /gene="atpA"
                     /locus_tag="OO544_001634"
     CDS             complement(11417..12958)
                     /gene="atpA"
                     /locus_tag="OO544_001634"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HCR6767254.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(12971..13504)
                     /gene="atpH"
                     /locus_tag="OO544_001635"
     CDS             complement(12971..13504)
                     /gene="atpH"
                     /locus_tag="OO544_001635"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HCR6767255.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(13519..13989)
                     /gene="atpF"
                     /locus_tag="OO544_001636"
     CDS             complement(13519..13989)
                     /gene="atpF"
                     /locus_tag="OO544_001636"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HCR6767256.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(14051..14290)
                     /gene="atpE"
                     /locus_tag="OO544_001637"
     CDS             complement(14051..14290)
                     /gene="atpE"
                     /locus_tag="OO544_001637"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HCR6767257.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(14337..15152)
                     /gene="atpB"
                     /locus_tag="OO544_001638"
     CDS             complement(14337..15152)
                     /gene="atpB"
                     /locus_tag="OO544_001638"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HCR6767258.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(15161..15541)
                     /gene="atpI"
                     /locus_tag="OO544_001639"
     CDS             complement(15161..15541)
                     /gene="atpI"
                     /locus_tag="OO544_001639"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HCR6767259.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAYTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            complement(16158..16781)
                     /gene="rsmG"
                     /locus_tag="OO544_001640"
     CDS             complement(16158..16781)
                     /gene="rsmG"
                     /locus_tag="OO544_001640"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HCR6767260.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            complement(16845..18734)
                     /gene="mnmG"
                     /locus_tag="OO544_001641"
     CDS             complement(16845..18734)
                     /gene="mnmG"
                     /locus_tag="OO544_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HCR6767261.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLVGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            complement(19113..19556)
                     /gene="mioC"
                     /locus_tag="OO544_001642"
     CDS             complement(19113..19556)
                     /gene="mioC"
                     /locus_tag="OO544_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HCR6767262.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLIISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            complement(19646..20104)
                     /gene="asnC"
                     /locus_tag="OO544_001643"
     CDS             complement(19646..20104)
                     /gene="asnC"
                     /locus_tag="OO544_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HCR6767263.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLAEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            20256..21248
                     /gene="asnA"
                     /locus_tag="OO544_001644"
     CDS             20256..21248
                     /gene="asnA"
                     /locus_tag="OO544_001644"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HCR6767264.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRENVPSLL"
     gene            complement(21253..22704)
                     /gene="viaA"
                     /locus_tag="OO544_001645"
     CDS             complement(21253..22704)
                     /gene="viaA"
                     /locus_tag="OO544_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HCR6767265.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(22698..24194)
                     /gene="ravA"
                     /locus_tag="OO544_001646"
     CDS             complement(22698..24194)
                     /gene="ravA"
                     /locus_tag="OO544_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HCR6767266.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRLQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSAQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIHQAQQC"
BASE COUNT         6003 a         6312 c         5775 g         6188 t
ORIGIN      
        1 acagcgtaac gtcctgtttg atgtagatgt cttgctatca cacctaaatg cgagggcaga
       61 cctaaggtag cgaccacctt gcggttgcca tacacctcat ccaacagctg cttaggacta
      121 acaatccctt gataaacgcc tgcccgtgca atagtgctat aaatcagctc attcgagtac
      181 ggaacaggaa agtttctcat ggctaacctg cttgtttaaa taagctcgcc atatccacta
      241 ttaccccttt cccttttaac cgttcatgca tggttttttc aggttggcgt tgtgaataga
      301 tataacgtaa atccgtatca ggcaagctat cccagtcgct gggcttgact acctttatag
      361 ccgaaacctt tttactcttg gagggctttt ctagttctga cactaccgtt tcgccttcca
      421 tcaaccactg caaaacaaga ggcagtaact tttgtcttgt catcgttgga ttctggctaa
      481 acgctttttt aatagtggga attaacaggc ttgaatcgta atcctctttc agcatcagat
      541 ataaatgacg ctgatcttcg gtatctaact cttgaagggc tttttcttct ggtgtttgtt
      601 cttgtatcgc tgcgatatct agctgaagtt ggattaaccg tttatcaatc tcgggaacga
      661 ctagatcaga ataacgagca atgcgttctg ggatacccga gcgtaatgcc tctagcatgg
      721 ggtgcacagg ctttaactca tcttgataca cttgccgcaa taaaccagcg gtaatacgct
      781 cattgcctaa agctagcgca cggagctgag cgagtacaaa aagttttact acaatgtcca
      841 tcactccttg gcttagctca taccacacat cacggacctc atccgataac agcgcatctt
      901 tgcgttgtaa aagctgtaat tgccaaagat tatccgtaaa agcgatccac tcttgattgg
      961 gctttccacg ttgcgtttgt tgtataggat cccagaatat agctccaaac cctgcccctc
     1021 tacgtgcaga ccgcaaatca gcctcaaaaa tctctcgtgc tttaggggta ccaatcaaca
     1081 tcactggtac gccaataata ttcaccatcg tcacaaaaaa gttcagcatc tcttgagatc
     1141 cacccgaacg agagcggctt aaatgctgaa tttcatcaat aaccaacaac cctaaagcat
     1201 gtgcattggc tatttgcgac atcaaagcca acatggtttc tataccatga cgttttaagc
     1261 cataacgacg ctcatagttc gagcccaagg ctcgatccaa cgctctgaaa aaattcaagc
     1321 agatttcttt tagcgaacca ttatgcgagc agtctatttt caaatacacc acctgctcta
     1381 cattgagttc acgatggtaa atcacctgag gatacgtggc tagaatacga tgaagagagg
     1441 tcgtcttccc actaccagaa caaccaatta acaataagct ttgtgccgta gatcgtgcct
     1501 cctcaaagcg aaatgtctcc aactctcccg tttgaacacg ctcataacca ttttgtaaat
     1561 gcttttgtaa atctcctgtt ttaggatttc tgcctacgta gccacctcga atcatgaccg
     1621 aaatacgctc acttagtagc aaatgcgtac ctaatggctg aaaatagtca tctggaatac
     1681 gacaaatggt atgagctctg ataacacggg acttttgcaa gtcagaggaa gtaagctgta
     1741 aagaggattt cagtgatgca gcactattca ctgactcttg taatggtggt aaggcctcga
     1801 taaaaggatt atcacgataa gcctctaccc ccgtatcacg ataaactgct tgaatccggg
     1861 tagcactcat gactcatcct tttctggtgg atcctgaaat aattcaggca cgtatgtggg
     1921 taggctgtaa tcttcttgat catccgcttc cactgcgttg aaaggaataa ctttagcctc
     1981 atcacctgaa gagcttggct tcaaatgctc cgcacgtttt ttacgctccg aggtcacggc
     2041 ttctttttta ttagttttaa tctgcttaat gcgtgttgat ttgggctcag tagtactggg
     2101 cgttaactta ttcgctttct gaatggtttg ctgaataaac gcctcaagct ccctgcgttt
     2161 agttaactca tcctgcttcg cattggcttt attgtgtttt tcttgtgctt gtatatccca
     2221 aacctcccaa aatgagagac ctttaaactg ccgactacgt tccgtcagat tacagcgcca
     2281 aaatacacgg ctgccaactt gcggaaacaa ataaatcgta tcaaccagca ctgggtcata
     2341 agccgcttct aaatgttgag gtctagctat atcagtgctc cgctgcaacc aaccctcacg
     2401 cagaatctct gaccccgagt aatacaaacc ccacaaatta acgccaaatg aagaaataga
     2461 gacctttcgg cgaggcagta acgctactcg caactgctct tgctccacag cccttaaact
     2521 acctgtacga tgctgcatac cccattgcca tagctggaca ggaatagacg gtaaatctgt
     2581 aggaaaatca gcatctcgat cgtatttatc catcaccaga tggttatttc tgaataagat
     2641 cgtacgcaaa ataatttgtg tgaactcaaa taccgacaga gatgcatcta acctatagtc
     2701 tgtttcacca tggcttttga tccgactgcc ctctacaatg ccaggtgcaa aggacttaaa
     2761 ctcggcttgt agtgttctaa aagtgctttc cactatgcct ttagcatcgc cacgtctagg
     2821 tggagcactt tccactcgca cattaaaact agaaactaag gcttcgacct gatgactcat
     2881 taattcgcca cggtccgcta gcaacacatc tggcaaacct acacacggcc agtctgagct
     2941 actaatctca atatcatgct gggcacaaat ggccgttttg tcagagcaag catttacaaa
     3001 agcctgcatc gccaccacat aagacggatt ttcaaagccg atataaaagc ccgtgatcat
     3061 ccgactaaac acatcaatca caatgtaaag cgttggtctt cctatgattt tttggcgatc
     3121 atgatgatcc actaaataaa tatcagcaat cgtggcatca atctcataac gactcccagg
     3181 gcctaacgcc tgagaagtgg ctgtactact taagggtcgt acgtcttttt tatatacccc
     3241 tgctttaact ctagacttta agcgctgagc tttagggtat tctcgatcat aaaaataacg
     3301 aaactgacgt agtgttgggt aatcctcttg gggaatgcga ggaaaatact gagcaaacaa
     3361 gtccacaaat cgtctatagg caacggtggt ctttgtacct ttttgattta acaggtgctt
     3421 ttctatggtc aacctaaaaa ggcgttcaat ctcgggcgtt accttggttc cttcaccctt
     3481 accatattct ctggctcggc caatctttgc tgttcctgtc gctgaacgtc tttcccctgg
     3541 tgcaccgctg tttttgtagt caggaattaa tgcattaggc gtttgaccac gctgccagta
     3601 acggcgtaac aacttataaa ctgtagcctt agtaacctta tgttcttgga ccacatgctc
     3661 aacgagttcg cttctgactt tagggtcgaa acgatcctta ctattaataa taggaagaat
     3721 ttttcgataa tcctcctcgc gcttctggaa gctgacagaa tctacagacg gctcttctaa
     3781 atcaagatgt acataagggt catcagttct aactaagcgg ccttcatcta aatactgcat
     3841 tagcaacaac tcagccctag cttgtggaac tcctttatca gcgcttattt gcatccagac
     3901 aacttggcca tcctctatag ccaaaatgcg atacggatca ttatcaaata gcacaacctc
     3961 attaatttgc cacatagcgc aactcctcca tattcactac ttggctaata cagagatctg
     4021 cacacttatt tgccctgaaa gacttataaa tattgaactt aataaaacca tttgcggtta
     4081 aggctcgtat ctcactcaat gttttgccta actccaaatc ataagcaata tcaacctgct
     4141 tacagacatt gataatgttt tcatctcctt tttcttgcag gatatgggcc aatggggata
     4201 gttgtgctaa aagctccgca gaaacttctt ctgttttcac tgaataaagc cattcaatat
     4261 tttcttttac tacgggattt atttctttat cagtaaaaat gaaccaagga atttgctttt
     4321 gctgccaata gcgacgctct agttctagtt tttctaaggt acgctcgtct tgtaaggctg
     4381 ctgcaggttt gacttgaata gcaaactgct caaaaggacc atctttgcag tccactaaaa
     4441 aatcagtaga cataacctga tctacaccac gaataacagg atgcttaata ccactatcta
     4501 ttgcaatctg cctggtatca ctaggtaata aggggaactg ctcgcgtata tctagcacgc
     4561 tgctctccca ctcaagactg agaaaaacag caagctctaa gtcagatagc aaatgatgga
     4621 ctcgtcccgt cttatgagaa taaatacggt gggaacgacc tgaagaagga acttcttgta
     4681 ctgttagcca tggaatatag tctttaccat gcccttggcc acgcccctct ttaatacgac
     4741 gggcaatttg cacttcagaa aatgaagagt ttgctttagc cataacaaaa gtccagtatg
     4801 ctttttcaca gcataactgg actgatttca gtttacaact attctgtcta gtttaagact
     4861 ttattgtcat agtttagatc tattttgttc agtttaagac tttattgtcc gcccacagcc
     4921 cttttttatc tatgatagtg attactcaac cgtaaccgat tttgccaggt tacgcggctg
     4981 gtcaacgtcg gtgcctttga tcagcgcgac atggtaagcc agcagctgca gcggaacggt
     5041 gtagaagatc ggtgcaatca cctcttccac atgcggcatc tcgatgatgt gcatgttatc
     5101 gctacttaca aaacccgcat cctgatcggc gaagacatac aattggccgc cacgcgcgcg
     5161 aacttcttca atgttggatt tcagtttttc cagcaattcg ttgttcggtg caacgacgat
     5221 aaccggcata tcggcatcaa taagcgccag cggaccgtgt ttcagttcac cagcagcgta
     5281 ggcttcagcg tgaatgtagg agatctcttt cagcttcaat gcgccttcca gtgcgattgg
     5341 gtactgatcg ccacggccca ggaacagtgc gtgatgtttg tcagagaaat cttctgccag
     5401 agcttcaatg cgtttgtcct gagatagcat ctgctcaata cggctcggca gcgcctgcag
     5461 accatgcaca atgtcatgtt caatggaggc atccagacct ttcaggcgag acagcttcgc
     5521 caccagcatc aacagcacag ttaactgagt ggtgaatgct ttagtggacg ccacgccgat
     5581 ttccgtaccc gcgttggtca tcagcgccag atcggattcg cgcaccagag aagaacccgg
     5641 aacgttacag attgccagcg aaccaaggta acccagctct ttcgacagac gcaggcccgc
     5701 cagggtatcc gcggtttcgc cagactgtga caaggtgatc atcaggctgt tacgacgcac
     5761 ggcagattta cgatagcgga attcagaggc gatttcgacg tcgcacggaa tacctgccag
     5821 cgattcaaac cagtagcggg aaaccatacc ggagttataa gaagtaccac aggcgaggat
     5881 ctgaatatgc tcaaccttcg acagcagttc gtcggcgttc ggtcccagct cgcttaaatc
     5941 aacctgaccg tggctgatgc gcccggtaag ggtgtttttt atcgcgttcg gctgttcgta
     6001 gatctctttc tgcatgtagt gacggtaaat acctttatcg cccgcgtcat attgcagatt
     6061 ggattcgata tcctgacgtt ttacttccgc gccagtttta tcgaagatgt ttaccgaacg
     6121 gcgagtgatt tccgcaatat cgccctcttc aaggaagata aagcgacggg tcaccggcaa
     6181 cagcgccagc tggtcagaag cgataaagtt ttcgcccata cccaggccaa tcaccagcgg
     6241 actaccagaa cgtgccgcca gcagggtatc cgggtgacgg gagtccatga tcactgtacc
     6301 gtacgcaccg cgcagctgcg ggatagcacg cagaacagcc tcacgcagag tcccgccttg
     6361 tttcagctcc cagttcacca gatgggcaat cacttcggtg tcggtttcag aaacgaaggt
     6421 atagccacgc gcttttaact cttcacgcag cggttcatgg ttttcgatga tgccgttatg
     6481 caccaccaca atgtgttcag aaacatgcgg atgcgcgtta gcttctgaag gttcaccgtg
     6541 ggtcgcccag cgagtgtgag caataccggt gccgccatcc agaggatgtt cttccgctgc
     6601 ctgagccagc atctggactt taccgaggcg acgcaggcgg gtcatatgac cttccgcatc
     6661 aacaacggcc agaccggcag agtcatatcc gcggtattcc agacgacgta aaccttcaag
     6721 aaggatttct gctacatcac gttgcgcgat cgcgccaaca attccacaca tagtttttga
     6781 ttccgattta tatcgttgtc ggtcaacctg tatgcccgtt ttttcgggcg ccccgagcct
     6841 tgtagagagt ggggttattt ttatagttac tgcttgtggg agggagatta tgttatctcc
     6901 tcatcccaga tgaccggatt acccggccag aattactttt tctttaccgg acgacgccag
     6961 ccttctttct gagtctgcgg cacacggctg atagctaatg cattttcgcc gacattacgc
     7021 gtcacagttg tacccgcagc aatggtcgcg cctttgccta ctgttaccgg ggccaccagc
     7081 tgagtgtcgg aaccgacaaa cacatcgtcg ccgataatgg tcttaaattt attcgcacca
     7141 tcgtagttgc aggtaatggt tcccgcgccg atgttaacgt tatcgccaat ttccgcatcg
     7201 cccaggtaag tcagatgccc gactttcgag cctttaccca gacgcgcttt tttcatctca
     7261 acgaagttac caacgtgtgc accttccagc aactcagcac caggacgcaa acgagcaaac
     7321 gggccaatag tacaggccgc tgccagattc gcgtcttcca caacggtata cggactgatt
     7381 tcgcaatcat cgccaatcac gctgttttta atcacgcaac cggtgccgat tttcacgcga
     7441 tgaccgagag tcacgttgcc ctcgatgata acgttagtat caatttcaac atcgcgcccg
     7501 tgagtaagcg taccgcgcag atcaaaacgc gccggatcgc gcagcataac gcctgctaac
     7561 agcagttttt cagcctgttc ggactggtaa acgcgctcca gacgggagag ttgcaggcgg
     7621 ttattcacac cttctacttc gcttaaacgt tgcggatgaa cggcgacgat ttcacgccct
     7681 tcctgatacg ccagcgcaat aatgtcggtg atgtagtatt cgccctgagc attattgttg
     7741 gtcagcttcg ccagccagcg tttcatatct gcgccgttag cgatcagaat gccggtgttg
     7801 atctcctgaa tctgacgctg ctcgtcggtg gcatctttgt gctcaacaat gccggtaact
     7861 ttgccatttt cacgggtgat acgtccataa ccggtcggat catccagttt caccgtcagc
     7921 agaccaatgc caccctgcgg tttagcatca cgcaggcgct ggagtgtttc gacagagatc
     7981 agcggcacgt cgccgtagag cattaaaatg tcttcatcat cggcaaagaa aggtgcggcc
     8041 tgctgcattg catgacccgt acccagctgc tctgcctgaa gcacccagtt aaggttgtcg
     8101 tctttcagcg cctgtttaag cagatcgccg ccgtgaccgt acaccaagtg aacgtgcgct
     8161 gcgcctaatt cattcgcagc atcaatgaca tgctgaacca tcgctttccc ggcaagggta
     8221 tgcagcactt tcggaagatc ggaatacatg cgcgtgcctt tgcctgcggc aaggatcact
     8281 acgctcatag cattattcaa catacgcgtc ctgactgtaa tttgagaacg aatttaaacc
     8341 gcttcacctt gaaaaaacta catttttttc atcgtgaaat ggacagagga taaattgttc
     8401 aataacggat tatccccgct gacgaaagtg ccattttcga ccatcgtcac gccttttgtc
     8461 tgcagaaaat taaggcagta ccttcattta agcgtcaggt ggatgttttt gctcttattt
     8521 cgatcaatga ataaacggga aaacaggggt gttttatatc agcaggatct atgtgaacgc
     8581 tcttcaggac ggatcacgcg taaaaaaaag ccagcctgtt tccagactgg cttttgtgct
     8641 tttcaagccg gtgttacatc gcttttttgg tcaactcgat aacgcgcagc tgcgcgatcg
     8701 ctttggccag ttccgcagac gcctgagcgt aatctacgtc gccgtgagag ctgctaatgt
     8761 gctcttcagc cttacgtttc gcttccatgg ctcgcgcttc gtcgagatcc tggccgcgaa
     8821 ttgcggtgtc ggccagaacg gtcacgttgc caggctgcac ttcaagaatg ccgccagaca
     8881 gatagataaa ctcttcgtga ccgtgctgtt tcacgatgcg aatcatacca ggcttaatgg
     8941 cggtgagcag cggtgcgtgg ccagggtaga tccccagttc accttcgcta cccgttacct
     9001 ggattttctc gaccagacca gagaacattt gttgctctgc gctgacgacg tccaggtggt
     9061 aagtcattgc catatcaccc tccgattaag gcgttaaagt tttttggctt tttccacagc
     9121 ttcttcgatg gaaccgacca tgtagaacgc ctgctccggc aggtgatcgt attcgccttc
     9181 catgatgcct ttaaagccac ggatggtgtc tttcagggag acgtatttac ccggagaacc
     9241 ggtgaatact tctgccacga agaacggctg ggacaggaag cgctggatct tacgagcacg
     9301 cgctaccacc agtttgtctt cttcagacag ttcatccata cccaagatgg cgatgatgtc
     9361 tttcagttcc tgataacgtt gcaggatgga ctgaacgcca cgcgcggtgt cgtagtgttc
     9421 ctgaccaacc accagcgggt ccagctgacg gctggtggag tccagcgggt caacggccgg
     9481 gtagataccc agagacgcga tctgacggct cagtaccacg gttgcgtcaa ggtgcgcaaa
     9541 ggtggttgcc ggagacgggt cagtcaagtc atccgcaggt acgtataccg cctgtacgga
     9601 ggtgatagaa ccagttttgg tggaggtgat acgttcctgc agaacgccca tctcttccgc
     9661 cagggtcggc tgataaccta ccgctgaagg catacggccc agcagtgcgg atacttccgt
     9721 accggccagg gtgtaacgat agatgttgtc aacgaacagc agaacgtcac gaccttcgtc
     9781 acggaatttc tcagccatgg tcagaccggt cagagcaacg cgcagacggt ttcccggcgg
     9841 ctcgttcatc tggccataca ccagggatac tttgtcgata acgttggagt cggtcatttc
     9901 gtggtagaag tcgttaccct cacgagtacg ttcacctacg cccgcaaaca cagagtaacc
     9961 ggagtgctcg atcgcgatgt tacgaatgag ctccatcatg tttacggttt tacctacacc
    10021 cgcaccaccg aacagaccaa ctttaccgcc cttagcgaac ggacacatca ggtcgataac
    10081 tttgataccg gtttccagca gttcctgaga gtttgacagc tcttcgtagg aaggtgctgc
    10141 gcggtgaatc gcccaacgct cttcttcacc gatctcgcct ttcatgtcga ccggttcacc
    10201 cagtacgttc atgatacggc ccagagtcgc tttacctacc gggacttcga tcgggtgttc
    10261 gaggtctttt acatccagac cgcgacgcag accgtcggag gaacccattg cgatggtacg
    10321 cacgataccg ccgccgagct gctgctgaac ttccagcacc agacgctcat taccattttg
    10381 cacctcaaga gcatcgtaca cgcgcggtac ggcatcctga gggaattcga cgtcaactac
    10441 ggcgccgatt acctggacaa tctttccagt agccatctta aatcctctac gaaataacct
    10501 gtttaaaccg cggcggcccc cgagacgatc tcggtgagtt cctgagtaat gctggcctga
    10561 cgagctttgt tgtataccaa ctgcagctct ttaatcaggc tgccgccatt gtcggtcgcg
    10621 gctttcatcg ccaccatacg ggcggcctgc tcgctggcca ggttttcaac cacgccctga
    10681 taaacctgag attcgacata acgacgcagc agggtatcca gcaacgcctt cggatcgggt
    10741 tcgtacaggt aatcccagga tttatgtttc agatcatcat catctgatgc cggtaacggc
    10801 agcagctggc tgatggtcgg aacctgagac atggtgttaa taaatttgtt gctgacaatg
    10861 taaagcttgt ccagacggcc ttcgtcgtaa gcctgcaaca tcacttttac cggaccgatc
    10921 agttcggaca gggaagggtt atcccccatg ccggtgacct gggcaacaac attgccgccc
    10981 acggagttga agaacgacac gcctttcgag ccgatcattg cgaggtcgca ttgaacgctt
    11041 ttgtcggtcc aggtcttcat ttccgccagt agttttttga acaggttaat gttcaaacca
    11101 ccgcacaaac cacggtcggt cgacaccacc aggtagccca cgcgtttaac gtcgcggtct
    11161 tccaggtaag ggtgcttata ttccagatta ccgtgtgcaa ggtgaccaat cactttgcgc
    11221 atggtttctg cataaggacg gctggccgcc atgcgatcct gcgatttacg cattttggaa
    11281 gcggcgacca tctccatcgc tttagtgatc ttttgcgtgt tctggacgct tgcgatctta
    11341 ctacgtatct cttttgcgcc ggccatgagc ttctcctcaa tgccttgcgg cctgccctaa
    11401 ggcaagccgc cagacgttac caggattggg ttgctttgaa ggaatcgagg atgcctttca
    11461 gcttgccttc gatttcgtcg ttgtagccac cggtctggtt gatctcttgc atcaacggag
    11521 cgtgatcacg gtcgacgtaa gccagcagag cggcttcgaa gctgccaatt ttcgacagtt
    11581 caacatccgc caggtaacca cgttctgctg cgaacagaac cagagactgc tgcgcaacgg
    11641 acatcggcgc atactgtttc tgtttcagca gttcggtcac tttctgaccg tggtcaagct
    11701 gcttacgtgt tgcatcgtca aggtcggatg caaactgaga gaacgctgcc agttcacgat
    11761 actgtgccag agcggtacgg ataccaccgg acagtttttt catgatcttg gtctgtgctg
    11821 caccaccaac acgggatacg gaaatacccg ggttaaccgc aggacgaata ccggcgttga
    11881 acaggttggt ttccaggaag atctgaccat cggtaatgga gattacgttg gtcggaacga
    11941 acgcagaaac gtcacccgcc tgagtttcga taatcggcag tgcggtcaga gaaccggttt
    12001 tccctttcac ttcacctttg gtgaaggctt caacgtattc ggcgttaaca cgtgcagcac
    12061 gctccagcag acgagagtgg aggtagaaaa cgtcgcccgg gaatgcttca cgtcctggcg
    12121 gacgacggag cagcagggag atctgacggt aagcaacagc ctgtttagac aggtcatcgt
    12181 aaatgatcag cgcatcttca ccgcggtcac ggaagtattc gcccattgcg caaccggcat
    12241 acggtgccag gtattgcagt gcagcggatt cagacgcggt tgctaccaca acgatggtgt
    12301 tagccagtgc gccgtgctct tccagtttac gtaccacgtt agaaatggtg gacgctttct
    12361 ggccgatagc gacatagata catttgatac cggaatcgcg ctggttgatg atggcatcga
    12421 tagccagtgc ggttttacct gtctgacggt caccgatgat caattcacgc tgaccacgac
    12481 cgattgggat catggagtca acggctttat aaccggtctg taccggctga tctacggact
    12541 gacgttcgat aacgcccgga gcgattgctt ctacagcaga gaagccgtcg tgatccagcg
    12601 gacctttacc gtcgattggt gcacccagag tgttaaccac acggcccagc aggccacggc
    12661 caaccggaac ttccaggata cggccagtac acttaacttt catgccttcg gcaaggtcag
    12721 cgtacggacc cataacaacc gcacctacag agtcgcgctc aaggttcagt gcgatagcgt
    12781 aacggttacc cggcagggag atcatttcac cctgcataca atcggccagg ccgtgaatgc
    12841 ggataacacc gtcacttaca gaaacaatag taccttcgtt gtgagcttca ctcacaacat
    12901 tgaactgagc aatgcgctgc ttgatcagtt cgctgatttc ggtggaattc agttgcatgc
    12961 tccagtcccc ttaagactgc aagacgtctg caaggcgctc aagacgaccg cgtacgctgc
    13021 catcaatgac catatcaccc gctcggatga taacgcctgc cattacagac ttatcgattt
    13081 tgcaattcag cttaactttg cgtgacagac gtttttccat cgcagcagaa attttcgcga
    13141 gctgttgttc actcagtgcg gcagcggaaa tgacgtctac ctcagcggta gcctcactca
    13201 cggcacgaag gtgaataaac tgctccagaa catccgggag cgcgttaaga cgaccatttt
    13261 cagccattac ccgaatcagg ttctgaccgt tttcgtccag ttgctcacca caaactgcga
    13321 taaacgactc ggcgagcgtt tctggcgcaa gcgcgccaga gagaagctct gccatttgtt
    13381 cgtttttggt tacctcggcg gcaaacgcca gcatgtcctg ccagcgttct acactttggt
    13441 gttcgacggc aaagtcaaaa gctgctttgg cgtaggggcg agctaccgta ataaattcag
    13501 acatcagccc ctccctcctt acagttcagc gacaagttta tccacgatgt cgctgttagc
    13561 agcttcatcc acggaacgtt cgatgatctt ctcggcgcca gcaacagcca ggatagcaac
    13621 ttgcttacgc agctcttcac gggcacgttt acgctcggct tcaatttctg cctgcgcctg
    13681 ggccacgatt ttagtacgtt cctgttctgc ctcagctttc gcttcgtcca gaatctgcga
    13741 gcggcgtttg ttcgcctgct cgatgattac ctgggcttcc gctttcgctt ttttcagctg
    13801 gtcggtcgcg ctggcctttg caaggtcaag gtccttatgt gctcgttctg cggaagcaag
    13861 gccgtcagca atttcttttt gacgtttttc gatggctgcc attaatggcg gccatacgta
    13921 cttcatgcag aacagaacga acaggacaaa cgcgatggcc tggccgagga ttgttgcgtt
    13981 aagattcaca gcacaatgcc tctatttagt taacgttctg atattgctct ttaaataaaa
    14041 gcaacgctta ctacgcgaca gcgaacatca cgtacagacc cagacctaca gcgatcatcg
    14101 ggatagcatc caccagaccc ataacgataa agaactgagt acgcagcaga ggaatcagat
    14161 caggttgacg cgctgcgcct tccaggaatt tacccccgag gatgccgata ccgatcgcag
    14221 caccgattgc cgccagaccc atcatcacag cggcagccat gtacagcaga tccatattca
    14281 ggttttccat gacagtctcc agtttgtttc agttaaaacg tagtagtgtt ggtaaattaa
    14341 tgttcttcag acgccatcga cagatagacg atcgtcagaa ccatgaagat gaaggcttgc
    14401 agcgtaatga tcaggatgtg gaaaatggcc cacggcacat tcaggatcca ctgtgaccac
    14461 cacggcaaca gaccagcaat cagaatgaaa atcagctcac cggcatacat gttaccgaac
    14521 agtcgcaaac cgagtgaaac tggtttggac agcaggctta ccccttcaag gattaagttg
    14581 acaggaatga acgcccagtg attgaacggc tgcagcgtca actctttcgt gaagccgccg
    14641 atgcctttca ttttgatgct gtagaacaga atcaggataa atacgcccag tgccatagac
    14701 agcgttacgt tcacgtccgc agacggaacc acacgcagtg caggcagacc cagtacatgt
    14761 tcagcaatgt acggcagcag gtcgataggc agtaaatcca tcaggttcat caggaatacc
    14821 cagacgaaga tcgtcagggc cagcggagca atcagcttgc ttttgccatg gtacatgtct
    14881 ttcacgctac cattaacaaa gccgatcacc agctcaatcg cggtctgaaa cttacctggc
    14941 acaccgctgg tcgccttttt ggctacgcta cggaataaaa ccaggaacaa cagacccagc
    15001 accaccgaga agaacatgga gtcaatattg attgtccaga aggtggctgg ggggttttgt
    15061 ggatccacca gcgagaatgt acgcaggtcc agctgaaggt tattcaggtg gtgtcctatg
    15121 taatcctgcg gcgtcatatt ttctgaagcc atgatgcctt ttaccctttg ttgttaatta
    15181 cagccggtgc cagtatctga accaccagca ccaaaaccca cgtaacgatc agcggcaaga
    15241 ataccgcctt taaaaccgcc aacgccacca ccagtaacac caacatcgcc agaactttga
    15301 aagcttcgcc aaatgcgaat gtccaggcca cccggccttt cgctggtgta tacgcctggt
    15361 gacgccaggc aaatatcata aacaaaacgt taggcagaaa gactgccagg cccccgctta
    15421 ttgcagagac gccccagaag gggtctttga ggctgaacag caatccactt gctatcacca
    15481 ccagtaactg aacgagcaga agcttccgag caacgtttcg actcacgagc gacacagaca
    15541 tcacgttttt cactcctgct cccttcgagg tatgccgcgt gtcgtataaa actttcttta
    15601 aggcttagag tcaagcatca aaaagcggtc aaattatacg gtgcgccccc gtgatttcaa
    15661 acaataagta gccaaaaggt gaataaatgt ttaaatattt ttccagtgca tacaattgcg
    15721 acttttccgc taaccctgtt cgatcatgaa aaactgtaaa taacgcgtaa acactagtga
    15781 taaagcgtgc ttcagatcac atattgcgca tgttcgcgca cagtatattt atttacttgg
    15841 caaatgatgc ctttgcaagt ttatgatatt tcagtctaaa aacagatact gttttaataa
    15901 atgacattta cacaacaaaa accacccatt gacattttta ataatgtttt aacagccaat
    15961 gatggttctt agcgccgatt tttagcagac tgatattttc actaatgact tattttctgc
    16021 ttaccaaaaa aagccacgtt atcttgttga tacaaaagag tgaacgtggc gttaaatgta
    16081 accagttata tcagtagaaa acttggttgt tgttaacagt ctaaccggtc aattttttat
    16141 gatttttttg ataaaaatta aattttattt gctttaatca ccaccagatg acgttcgcca
    16201 tccagagctg gaacctgaag tttaaccact gattcgacct gatattcttc gggcaacaaa
    16261 gcgatttcat cttccggcat ttgccctttc agcgcgtaga aacggccttg ctcaccagga
    16321 agatggtggc accagctcac catatcgttc agagaggcaa aagcgcggct aattacgcca
    16381 tcaaatggcg gctctgaagg aaactcttct accctgctct gtactggctc aatattctcc
    16441 agtttaagct catgttgcac ctgacgaagg aaacgcacgc gtttaccaag gctatccaac
    16501 agagtgaaat gggcttcagg acgcacgata gagagtggaa tgcctggcag tcctggtccg
    16561 gtgccgacat cgataaaccg ttcaccttgc agatacggtg ccaccacaat gctatcgaga
    16621 atatggcgta ccagcatctc attaggatcg cggaccgaag tcaggttgta cgctttgttc
    16681 catttatgca gcatattcac gtaggcaata agctggtttt tctggtgatc ggtaagcgaa
    16741 atacctgcgt ctttcagcag taaggagagt ttgttgagca cggtgattac ctgttcttga
    16801 tgcgttgcct ggtaagcggg tgcttaccag gcatttttaa tgcgttatgc gctacgacgc
    16861 agcataccct gttttttcag ccacaccagc agaatggaga tagccgcagg cgtgacgcca
    16921 gaaatacgcg aagcctggcc gatagaggcc ggtttgtgat cgttaagttt ggcgatcact
    16981 tcgttagaaa gaccggatac ctggcggtaa tccagtgtcg cgggtagcag ggtgttctcg
    17041 ttacgcagct gcttttcgat ctcatcttgc tggcgcgcga tataaccttc gtatttaacc
    17101 tgaatctcaa cctgttccgc cgcctgttcg tctgtcaacg caggggcaaa cggcgtcagc
    17161 gtggttaatt tttcataagt catttccgga cgacgcagca gatcttcacc actggcttca
    17221 cgggaaagcg gcgcagtcag gtgagcattc acttcggctg cagcttccgc cgacggggtt
    17281 acccaggtcg atttcagacg ctgacgctca cgctcgatat tctcaagttt ctcgttaaag
    17341 cgcgcccaac gttcgtcatc caccaggccc agttcacgac cgatttcagt caaacgcaga
    17401 tccgcattat cttcgcgcag catcagacga tattctgcgc gcgaagtaaa catacgatac
    17461 ggttctttgg ttcctaaagt gcacaggtca tcaaccagta cgccgagata cgcctgagaa
    17521 cgcgccggag cccaaccttc tttgtcagca gacagacggg cagcgttaag accgaccagc
    17581 aaaccttgcg cagcggcttc ttcgtaaccg gtagtgccgt taatctgacc agcaaagaac
    17641 agcccctgga taaacttgct ctccagcgtc ggtttcaggt cgcgtggatc gaagaagtca
    17701 tactcaatgg cataacccgg acgcacgatc ttcgcgtttt ccatcccctg catggagcgg
    17761 acgatttgca tctgcacatc gaacggcagg ctggtggaga taccgttcgg ataaatttcg
    17821 ttagaggtca gcccttccgg ttcaaggaag atctgatgct gatttctgtc ggcgaagcgc
    17881 atgactttgt cttcgatcga tgggcagtag cgtgggccga caccttcgat cacccctgcg
    17941 tacattgggc tacgatcgag gttactgcgg atcacatcat gggttttctc gttggtatga
    18001 gtgatataac acggcacctg ctggggatgc tgggacgcat tgcccataaa cgagaatacc
    18061 ggcattgggt tatcgccatg ctgttgcgcc agcacactaa agtcgatagt tcgagcatca
    18121 atacgcggtg gtgtcccggt tttcagacga ccaacgcgca gcggcagttc acgcaaacgg
    18181 cgagaaagcg gaatggacgg cggatcacca gcacgaccac cgctgtaatt atccagaccg
    18241 atatgaattt taccgtcgag gaacgtccca acggtgagca cgacggcttt ggcacggaac
    18301 ttcagtccca tttgggtaac agcaccgacc acgcgatcgt tttcgacaat aagatcttca
    18361 accgcctgct ggaagatcat caggttcggt tggttctcca gcgccgtacg taccgcctga
    18421 cggtagagca cacgatccgc ctgagctcgg gtagcgcgaa ccgccggtcc tttgcttgcg
    18481 tttagtatcc taaactggat acccgcttga tcgatcgctt tcgccatcag accgccgagt
    18541 gcatccactt cttttaccag atgtcccttc ccaataccgc cgatcgccgg gttgcagctc
    18601 atctgcccca gagtgtcgat attgtgtgtc aaaagcagag tctgttgacc catacgcgcc
    18661 gcggccatcg cggcctcggt gcctgcatga cccccgccaa tgatgatgac gtcaaaagga
    18721 tccggataaa acatggtgat tgcctcgcat aacgcggtat gaaaatggat tgaagcccgg
    18781 gccgtggatt ctactcaact ttgtcggctt gagaaagacc tgggatcctg ggtattaaaa
    18841 agaagatcta tttatttaga gatctgttct attgtgatct cttattagga tcgcactacc
    18901 ctgtggataa caaggatccg gcttttaaga tcaacaacct ggaaaggatc attaactgtg
    18961 aatgatcggt gatcctggac cgtataagct gggatcagaa tgaggggtta tacacaactc
    19021 aaaaactgaa caaccgttgt tccttggata actaccggtt gatccaagct tcctgacaga
    19081 gttatccaca gtagatcgca cgatctgtat acttatttga gtaaattaat ccacgatccc
    19141 agccattctt ctgccggatc ttccggaatg tcgtgatcaa gaatgttgat cttcagtgtt
    19201 tcgcctgtct gttttgcacc ggaatttttg agttctgcct cgagtttatc gatagcccca
    19261 caaaaggtgt catattcacg actaccaata ccgattgcgc caaagcggac cgcagaaaga
    19321 tcgggcttct gttcctgcaa tgcttcatag aaaggagaaa ggttgtccgg aatatctccg
    19381 gcaccgtggg tggagctgat aatcagccag atccccgagg cagataaatc ttccaacagc
    19441 ggaccgtgca gcgtttcggt ggtaaaaccc gcctcttcca gcttttcagc caggtgttct
    19501 gctacatatt cggcaccgcc gagggtgctg ccgctgataa gagtgatatc tgccataaac
    19561 cgccaccttt attaagagtg gcgtattgta cgctgtgaac gcgttgggat ctacctgtgg
    19621 aaaagtatgg gattaaaaaa gccgattatg gcttgatggt acgcatgatc gggttttgca
    19681 ggacgatcaa tgtctcggtg gactgaattt catcaattgt ttggatcttg ttgataagta
    19741 catgctggag agcgtcgatc gaacggcaca ttacttttat aaagatgctg tagtggcctg
    19801 ttgtgtaata ggcttcagtg acttcagcaa ggctttccag ctttgccagc gcggaagggt
    19861 agtctttggc gctctttaat ataatgccga taaagcagcc tacgtcataa ccgagctgct
    19921 tcgggctgac atcaatacgc gccccggtaa tgatccccgc ctgcttcatt ttctctactc
    19981 gaacgtgaat cgtccccgga ctgacgccaa attgtttcgc cagttcggcg taagcggtgc
    20041 gcgcattgcc cattaatgct tccaggatgc cacggtccag attgtcgatc agataatttt
    20101 ccataggatt ttcttatgca gattgatgat tcattctatt ttagccttct tttttaatga
    20161 atcaaaagtg tgttaggctt tttattgaat gattattgca tgtttgtcgg tttttgttgc
    20221 ttaatcataa gcaacaggac gcaggagtat aaaaaatgaa aaccgcttac attgccaaac
    20281 aacgccaaat tagcttcgtg aaatctcact tttctcgcca actggaagaa cgtctggggc
    20341 tgatcgaagt ccaggcgccg attcttagcc gtgtggggga tggcacgcag gataacttgt
    20401 cgggctgtga aaaagcggtg caggtaaaag tgaaagctct gcctgatgcc cagttcgaag
    20461 tggttcattc actggcgaag tggaaacgtc agaccttagg gcaacacgac ttcagcgcgg
    20521 gcgaagggct gtacacgcac atgaaagccc ttcgccccga tgaagaccgt ctttctccgt
    20581 tgcactcggt ctatgttgac cagtgggact gggaacgcgt aatgggcgac ggtgagcgtc
    20641 aattctcgac tctgaaaagc acggtagagg cgatctgggc gggaattaaa gcaaccgaag
    20701 ctgcggttag cgaagagttt ggcctggcac cgttcctgcc ggatcagatc cacttcgtac
    20761 acagccagga gttactgtct cgttatccgg atctcgatgc caaagggcgt gagcgggcga
    20821 tagcgaaaga tcttggcgcg gtattccttg tcgggattgg cggcaagctg agcgatggtc
    20881 atcgccacga cgtgcgcgca ccggattatg atgactggag caccccgtca gagctgggtc
    20941 atgcgggtct gaacggcgat attctggtgt ggaaccctgt actggaagat gcgtttgagc
    21001 tttcttccat ggggatccgc gtggatgccg acacgttgaa gcatcagttg gcgctgaccg
    21061 gtgatgaaga tcgtctgcag ctggaatggc atcaggcgct gctgcgcggt gaaatgccgc
    21121 agaccatcgg cggcggtatc ggccagtctc gtttgaccat gctgctgctg caactgccgc
    21181 atatcggcca ggttcagtgt ggtgtatggc cagctgctgt tcgcgagaac gtcccttctc
    21241 tgctgtaata atttatcgcc gccagcgtct gagcaggcgg cttcgcatcc cggtatcaaa
    21301 gcgccagata tgatcgaaaa tgcgcatgat gccgggtttg ccgtgtgccg acatcgccac
    21361 ggcatgaaag cgatgctgat gtacccgctg cagctctttc actttactcg tcacgtcgtc
    21421 aggcaaccgc tgagcgataa aatcagaaat caccaccgca tcggcatcaa accattccct
    21481 gctttgcaag cgttccataa tggcgcgaaa acaactggca agatcggtgc cgccacgaaa
    21541 ctgctggctt aaaaaacgga ttgcttgttc gatgccttgg gggcctgaaa gctcataacg
    21601 gacgatctcg gtggaaaata gcataatata gcagcgccgg ttttcagcga gagcaatgcg
    21661 catcaaggcc aggcagaacg ctttcgcaca ctgttcatta aagccgccca ttgagccgga
    21721 agtatccaca cagacaataa acggcccgcg cggctgttca tcgtaatctt tatgtaccac
    21781 cggacgttcg atcacttttt cacgccacga ctcaccgtgc aggcgatagg tgagcaactg
    21841 tttttccacc agccgacggt aaaactcata ctccagttcc gttatcccta gtgtcgccag
    21901 ttctggcggc agaagacgta aaatatcatc gctttgttgc agaccatcaa cctgctcagg
    21961 aaccgtcgcc ggttcgcgca ccatggtgcg gaaggtttcc atctgcgcat cgttgcgcgg
    22021 tattgatttg gcttcccgag aacgccccag ctgctctgcc aggcgtttca gttccggctg
    22081 ttcgttaaga aattcaccgt atttcacaat caactgatag tcgccacgtt taagctgacc
    22141 ggcgctcata tcccacagac gaccagctgc agtattgtta tctgcgagaa tcggttcaag
    22201 ttgtccgctc agcgtcatgc gttcttgaac ttcactcaac agttgttcgc gttcttcttc
    22261 taatagctgt tgatttaacg tcgttgcttg cacgatcaga cttaaacgcc aacgctggag
    22321 aaaaagcgtg tgtaacgctg aagttattgt gctgttagca tcaaccaact gccgggcttg
    22381 ttctgcccat ggggaattca gacgatgcag taagtccagg atctgtggta gctgcacaat
    22441 aaactgcggc gtggagagga gctggctttg ctgatagcac atcacctctt cggtgagttc
    22501 cggcgggact cgggcatctt tcagccgact gcgcagcgcc tcacgccagc ggggaacatc
    22561 atcagtgatt gccgccttca ggcgtgggaa tttttcaaag aagaccgcca gctgcggtga
    22621 ggccagcagt gcgatgatca tctcttcgat caatccctct tcgctgacgg ccagcatcac
    22681 attaagcgta tccagcgtta gcattgttgt gcctggtgaa tctgtgcacc gacatcctgt
    22741 aggctggctt caatgcgacc taaccagtcg ccagggataa acagacattt ttgctgttcg
    22801 ctgaataaag cgtgttgctt gcgccagtcg ctttcaagct cttccagttg ttgttttatt
    22861 tcaccaggca gaccttcagc cgatgaaccg ggaagtgcca gcgtactgcc ttgtaaactt
    22921 acatcgcgaa cgacaagatg ttgggcgcta tcaacttcca gattcagttt ctgggcaaag
    22981 ccgataccgt tcagtttacc gcgaatttca ccacctttgc tcagccactg ttccagcgcg
    23041 ctacgctcaa aggagatatg aaccacttcc atatcatgca gttttaacgg tttttgcagc
    23101 agtagagtca gagtggaagc agtaacgtta acaggaagtt gatactgctg acgacggctg
    23161 aaaatgccgc ccagacgaat taccgttaag gctgttttat cgctttgttg ctgctggagt
    23221 tgcaggtgac gttgcacaat cgcgccaagg cgagtcaaca tcccttgctg ttgccaggca
    23281 tgaccggtca tcaatacatc aatttgttgt tgtatcaaat tcaggctttg cgcgtcatac
    23341 cacaggcaat ctttcagcaa aatgagatct accggggcaa cagcactgcg accgctaaaa
    23401 aaggcgctgg cctgcaataa tcggatcgct tttttccagc gacgatccga gacataaggc
    23461 gcatccggta atttatccag ttgcaggcgc agcataaaaa tcagctcaaa tacatgatcg
    23521 ggcagcgtaa tttcaccaat ctctttctgc cagcgttcat attcttcatc tgtgacctgc
    23581 aaggcatcag gaaccggatt gtcgttttca tcctgttgac tggtcagcat ggagcggaaa
    23641 ttcgctttat cctgcacttt atctaaccac agacgaatca gcatgcggtc atataacgct
    23701 tccagactgc tgtctgcttc cggcagctcg ttggaggccg ccaccagcag acgcatcggg
    23761 attttttcta cgtgtgcacc gttgcggaac tggcgctcgt taatggcggt gagcaaggta
    23821 ttaagaattg ccgggcccgc tttccagatc tcatccagaa agacaatttc tgcttccggc
    23881 aggtaaccgc tggttaaacg ttcatagcgc ccttcatctt ttagcgcctg aatagaaagg
    23941 ggaccaaaaa cttcttccgg cgtggagaag cgggtcatca gatattcaaa cgcgcgggca
    24001 ttctgaaagg cgaattttaa gcgccgggcg atcaaacttt tggcaatacc tggcgggcca
    24061 aggaggaaca cactttcacc acttaatgcc gctaataaac acaagcggat ggcgtggcta
    24121 cgttcataaa gccccttttc caacgaactg ctcaggcggg aaattctttc cgctaataaa
    24181 tgagggtgag ccataatgaa gtggcgtcct ttcgtcaaaa gttctgcgta aattgcgagt
    24241 atagacgttt cttgctggtg gctaaaatag tctcaaag
//
LOCUS       DAJTZL010000046        24191 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.046, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000046 DAJTZL010000000
VERSION     DAJTZL010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24191
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.046"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..2518
                     /gene="rbbA"
                     /locus_tag="OO544_001647"
     CDS             <1..2518
                     /gene="rbbA"
                     /locus_tag="OO544_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HCR6767267.1"
                     /translation="GDMRDPRHRRDVCPRIAWMPQGLGKNLYHTLSVYENVDFFARLF
                     GHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCCALIHDPELLILDEPT
                     TGVDPLSRSQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWLVAMNAGEVLATGSAE
                     ELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIAIEARDLTMRFGSFVA
                     VDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAWLFGQPVDPKDIDTRR
                     RVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMSERFKLNDVEDVLPES
                     LPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLMVDLSRQDKVTIFIST
                     HFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFIAYLQEAAGQSNEAEA
                     PPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALMGTVILMLIMGYGISM
                     DVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDELDRRMRAGDITVAIEI
                     PPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWLQDVASRQSTPASQSG
                     LMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVREKELGSIINLYVTPT
                     TRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTLTLAALLYIIIATGMG
                     LLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGRWIGEVYPTSHFLTIA
                     RGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            2518..3642
                     /locus_tag="OO544_001648"
     CDS             2518..3642
                     /locus_tag="OO544_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6767268.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMAKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            3715..3988
                     /locus_tag="OO544_001649"
                     /pseudo
     CDS             3715..3988
                     /locus_tag="OO544_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
     gene            3985..4344
                     /locus_tag="OO544_001650"
     CDS             3985..4344
                     /locus_tag="OO544_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HCR6767269.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(4464..4865)
                     /gene="nikR"
                     /locus_tag="OO544_001651"
     CDS             complement(4464..4865)
                     /gene="nikR"
                     /locus_tag="OO544_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HCR6767270.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(4871..5677)
                     /gene="nikE"
                     /locus_tag="OO544_001652"
     CDS             complement(4871..5677)
                     /gene="nikE"
                     /locus_tag="OO544_001652"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HCR6767271.1"
                     /translation="MTLLNVSGLSHHYAHGGFNGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPAQGNISWRGEPLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQVVGDKLTFSSDAGRVLQNAVLPAFPVRRRTTEKV"
     gene            complement(5674..6438)
                     /gene="nikD"
                     /locus_tag="OO544_001653"
     CDS             complement(5674..6438)
                     /gene="nikD"
                     /locus_tag="OO544_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HCR6767272.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKAVYPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHAVTRSLVSAHLALYGMELAS"
     gene            complement(6438..7271)
                     /gene="nikC"
                     /locus_tag="OO544_001654"
     CDS             complement(6438..7271)
                     /gene="nikC"
                     /locus_tag="OO544_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HCR6767273.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(7268..8212)
                     /gene="nikB"
                     /locus_tag="OO544_001655"
     CDS             complement(7268..8212)
                     /gene="nikB"
                     /locus_tag="OO544_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HCR6767274.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(8212..9786)
                     /gene="nikA"
                     /locus_tag="OO544_001656"
     CDS             complement(8212..9786)
                     /gene="nikA"
                     /locus_tag="OO544_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HCR6767275.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTPQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKELLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(9897..10484)
                     /gene="acpT"
                     /locus_tag="OO544_001657"
     CDS             complement(9897..10484)
                     /gene="acpT"
                     /locus_tag="OO544_001657"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HCR6767276.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(10539..11588)
                     /locus_tag="OO544_001658"
     CDS             complement(10539..11588)
                     /locus_tag="OO544_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR6767277.1"
                     /translation="MEIPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLELM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            11720..12937
                     /locus_tag="OO544_001659"
     CDS             11720..12937
                     /locus_tag="OO544_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6767278.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVNY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKVGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            complement(12941..13498)
                     /gene="dcrB"
                     /locus_tag="OO544_001660"
     CDS             complement(12941..13498)
                     /gene="dcrB"
                     /locus_tag="OO544_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HCR6767279.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(13571..14236)
                     /locus_tag="OO544_001661"
     CDS             complement(13571..14236)
                     /locus_tag="OO544_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HCR6767280.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSILGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNILLKRLADKSEINAL
                     QAS"
     gene            14457..14702
                     /gene="tusA"
                     /locus_tag="OO544_001662"
     CDS             14457..14702
                     /gene="tusA"
                     /locus_tag="OO544_001662"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HCR6767281.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            complement(14804..17002)
                     /gene="zntA"
                     /locus_tag="OO544_001663"
     CDS             complement(14804..17002)
                     /gene="zntA"
                     /locus_tag="OO544_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HCR6767282.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(17076..17702)
                     /locus_tag="OO544_001664"
     CDS             complement(17076..17702)
                     /locus_tag="OO544_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HCR6767283.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIIRSLYL"
     gene            17843..18202
                     /locus_tag="OO544_001665"
     CDS             17843..18202
                     /locus_tag="OO544_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HCR6767284.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(18205..18474)
                     /locus_tag="OO544_001666"
     CDS             complement(18205..18474)
                     /locus_tag="OO544_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HCR6767285.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(18464..19060)
                     /gene="rsmD"
                     /locus_tag="OO544_001667"
     CDS             complement(18464..19060)
                     /gene="rsmD"
                     /locus_tag="OO544_001667"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HCR6767286.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            19210..20706
                     /gene="ftsY"
                     /locus_tag="OO544_001668"
     CDS             19210..20706
                     /gene="ftsY"
                     /locus_tag="OO544_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HCR6767287.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     VKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVVESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVKAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            20709..21377
                     /gene="ftsE"
                     /locus_tag="OO544_001669"
     CDS             20709..21377
                     /gene="ftsE"
                     /locus_tag="OO544_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HCR6767288.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSHRMLTLSDGHLHGG
                     VGHE"
     gene            21370..22428
                     /gene="ftsX"
                     /locus_tag="OO544_001670"
     CDS             21370..22428
                     /gene="ftsX"
                     /locus_tag="OO544_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HCR6767289.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINDLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            22673..23527
                     /gene="rpoH"
                     /locus_tag="OO544_001671"
     CDS             22673..23527
                     /gene="rpoH"
                     /locus_tag="OO544_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HCR6767290.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            23798..>24191
                     /gene="livJ"
                     /locus_tag="OO544_001672"
     CDS             23798..>24191
                     /gene="livJ"
                     /locus_tag="OO544_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HCR6767291.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTA"
BASE COUNT         5672 a         6354 c         6738 g         5427 t
ORIGIN      
        1 cggcgatatg cgcgacccga ggcatcgccg cgacgtctgc ccgcgcatcg cctggatgcc
       61 gcaggggctg ggcaaaaacc tctaccacac cttgtcggtg tatgaaaacg tcgatttttt
      121 cgctcgcctg ttcggtcacg acaaagcgga gcgggaagtg cgaattaatg agctgctgac
      181 cagcacgggg ttagcaccgt ttcgcgatcg tccggcaggg aaactctccg gcgggatgaa
      241 gcaaaaactt gggctgtgct gcgcgttaat ccacgacccg gaactgttga tccttgatga
      301 gccaacaacg ggggttgacc cgctctcccg ctcccagttc tgggatctga tcgacagtat
      361 tcgccagcgg cagagcaata tgagcgtgct ggtcgccacc gcctatatgg aagaggccga
      421 acgcttcgac tggctggtag cgatgaatgc cggagaagtg ctggcaactg gcagcgccga
      481 agagctacgg cagcaaacgc aaagcgctac gctggaagaa gcatttataa atctgttacc
      541 gcaagcgcaa cgccaggcgc atcaggcggt agtgatccca ccgtatcaac ctgaaaacgc
      601 agagattgcc atcgaagcgc gcgatctgac catgcgtttt ggttccttcg ttgccgttga
      661 tcacgttaat ttccgcattc cacgcgggga gatttttggt tttcttggtt cgaacggctg
      721 cggtaaatcc accaccatga aaatgctcac cggactgctg cccgccagcg aaggtgaggc
      781 gtggctgttc gggcaaccgg ttgatccaaa agatatcgat acccgccgtc gggtgggcta
      841 tatgtcgcag gcgttttcgc tctataacga actcaccgtg cggcaaaacc ttgagttaca
      901 tgcccgtttg tttcacatcc cggaagcgga aattcccgca agagtggctg aaatgagcga
      961 gcgttttaag ctcaacgacg ttgaagatgt tctgccggag tcattgccgc tcggcattcg
     1021 ccagcggctt tcgctggcgg tggcggtgat tcatcgcccg gagatgttaa tcctcgatga
     1081 gcctacttct ggtgtcgatc cggtggcgag ggatatgttc tggcagttga tggtcgatct
     1141 ctcgcgccag gacaaagtga ctatcttcat ctctacccac tttatgaacg aagcggaacg
     1201 ttgcgaccgc atctcactga tgcacgccgg aaaagtgctt gccagcggta caccgcagga
     1261 actggttgag aaacgcggag ccgccagtct ggaagaggca tttatcgcct atttgcagga
     1321 agcggcaggg cagagcaacg aagccgaagc gccgcccgtg gtacacgaca ccacccacgc
     1381 gccgcgtcag ggatttagcc tgcgccgtct gtttagctac agccgccgcg aagcgctgga
     1441 actgcgacgc gatccagtac gttcgacgct ggcgctgatg ggaacggtga tcctgatgct
     1501 gataatgggt tacggcatca gtatggatgt ggaaaacctg cgctttgcgg tgctcgaccg
     1561 cgaccagacc gtcagtagcc aggcgtggac actcaacctc tccggttccc gttactttat
     1621 cgaacagccg ccgctcacta gttatgacga gcttgatcgt cggatgcgtg cgggcgatat
     1681 cacggtggcg attgagatcc cgcccaattt cgggcgcgat atcgcgcgtg gtacgcctgt
     1741 ggaactcggc gtctggatcg acggagcgat gcccagtcgt gccgaaacgg taaaaggtta
     1801 cgtgcaggca atgcaccaga gttggcttca ggatgtggcg agccgacaat cgacacccgc
     1861 cagccaaagc gggctgatga acattgagac gcgctatcgc tataacccgg acgtaaaaag
     1921 cctgccagcg attgttccgg cggtgatccc gcttctgctg atgatgatcc cgtcaatgct
     1981 aagcgccctt agcgtggtgc gggaaaaaga gctagggtcg attatcaacc tttacgtgac
     2041 ccccaccacc cgtagcgaat ttttgcttgg caaacagctg ccgtacatcg cgctggggat
     2101 gctgaacttt ttcctgctct gcggcctgtc ggtgtttgtg tttggcgtac cgcataaagg
     2161 cagtttcctg acgctcaccc tggcggcgct gctgtatatc atcattgcca ccggaatggg
     2221 gctgctgatc tccaccttta tgaaaagcca gattgccgcc attttcggaa cggcgattat
     2281 cacgttgatc ccggcgacac agttttccgg gatgatcgat ccggtagctt cgctggaagg
     2341 gcctggacgt tggatcggcg aggtttaccc gaccagtcat tttctgacta tcgcccgcgg
     2401 aacgttctcg aaagcgctgg atctgactga tttgtggcaa ctttttatcc cgttgctgat
     2461 agccatcccg ctggtgatgg gcttaagcat cctgctgctg aaaaaacagg agggatgatg
     2521 cgccatttac gcaatatttt taatctgggt atcaaagagt tgcgcagtct gctcggtgat
     2581 aaagcgatgc tgacgctgat tgtcttctcg tttacggtgt cggtgtattc gtcagcgacc
     2641 gttacgccag gatcgttgaa cctcgcgccg atcgccattg ccgatatgga tcaatcgcag
     2701 ttatcgaacc ggatcgttaa cagcttctat cgtccgtggt ttttgccacc ggagatgatc
     2761 accgccgatg agatggatgc cggactggac gccggacgct ataccttcgc gataaatatt
     2821 ccgcctaatt ttcagcgtga tgtcctcgcc ggacgccagc cggatattca ggtgaacgtc
     2881 gatgccacgc gcatgagcca ggcatttacc ggcaatgggt atatccagaa tattatcaac
     2941 ggtgaagtga acagctttgt cgcgcgctac cgtgataaca gcgaaccgtt ggtatcgctg
     3001 gaaacccgga tgcgctttaa cccgaacctc gatcccgcgt ggtttggcgg ggtgatggcg
     3061 atcatcaaca acattaccat gctggcgatt gtattgaccg gatcggcgct gatccgcgag
     3121 cgtgaacacg gcacggtgga acacttactg gtgatgccga taacgccgtt tgagatcatg
     3181 atggcgaaga tctggtcgat ggggctggtg gtgctggtgg tatcgggatt atcgctggtg
     3241 ctgatggcga aaggtgtact gggcgtaccg attgaaggct cgatcccgct gtttatgctg
     3301 ggcgtggcgc tcagtctgtt tgccaccacg tcaatcggca tttttatggg gacgatagcg
     3361 cgttcaatgc cgcaactggg gctgctggtg attctggtgc tgctgccgct gcaaatgctt
     3421 tccggtggat ccacgccgcg cgaaagtatg ccgcagatgg tgcaggacat tatgctgacc
     3481 atgccgacga cacactttgt tagcctcgcg caggccattc tctaccgggg tgccggattc
     3541 gaaatcgtct ggccgcagtt cctgacgctg atggcaattg gcggcgcgtt tttcaccatt
     3601 gcgctgctgc gattcaggaa gacgattggg acaatggcgt aaggaaggga aacttgactt
     3661 catactgtga tgtcatacta ttgatgtcat aaagtgaagt cataaggaca tgccatgaga
     3721 accaaggtac aggctttacg gaagaaaaaa aaatacattg gatcaaatat ttaaaactcc
     3781 tgttcctcaa gggatcaagt ggtctgatat agagtcactg gttaaagcat taggcggaga
     3841 aattaaggaa ggaagaggtt cgcgttgtaa gttcatacta aatatgagcg ttgcgtgttt
     3901 ccatcggcct catccgtcgc cagataccga taaaggcgct gtagaaagcg tgcgtgactg
     3961 gttactaagt ataggggtaa aaccatgatc aaactgaaga cgccaaactc tatggagatt
     4021 gccgggcaac cagctgttat tacctacgtg ccagaactga atgcatttcg tggcaaattc
     4081 ctgggcttgt caggttattg tgattttgtc tctgacagca ttcaggggct gcaaaaagaa
     4141 ggggaacttt ccctgcgaga gtatcttgag gattgtaaag ctgcgggtat tgagccgtat
     4201 gcgcgtacag aaaagattaa gacctttacg ttacgctatc cagaatcgct gagtgaacgc
     4261 cttaataatg cggctgcgca gcaacaagtc tctgttaata cctatattat tgaaacactg
     4321 aatgagcgtt tgaatcattt ataaatttta gcggcgtaca ttacgcagaa tatctgcctt
     4381 tacaggatct atctatgcaa ctgaacttaa ttttataaat taagttcagt taatatcttt
     4441 tctttcctcg tttcagaaat aactcaatct tccttcggca agcactgcaa atgcccatgc
     4501 cgcacgccgc gctgggcgat aacgtcatcg gcaaaatgct gcacgtcgcc catatcacct
     4561 ttcagcacgg cgatttccag acagtcgtcg tgattgatat gcacatgcag cgtggcgacg
     4621 gagagatcgt ggtgatggtg ctgggtggag acaatgcggc tggctaagtc gcgtttttcg
     4681 tgttcataca cataggagag caccgcgaaa ccttgcgtgc cgtgctgctg agtggcctcc
     4741 tgggccaggg cgctacgcag aatatcgcgg atagcttcgg aacggttgtt ataaccacga
     4801 cgctggctca ggctgtccag cgtctccagt aaatcgtcat caagcgtgat ggtgactcgt
     4861 tgcatttgcg ttaaaccttt tctgtggtgc gacggcgcac ggggaatgcg ggtaataccg
     4921 cgttttgtag cacacgtccg gcgtcagagg aaaaggttaa tttatctccc accacctggg
     4981 tttcgacgat ttgtccgttg tccatcacca tcacccgctg acagaagcgt tccaccaggc
     5041 gtaagtcgtg ggtgatgaac aggcaggcgg tgccaaactg ttgttgtagc tttttcagca
     5101 ggcgaatgac gcccgcctgt aacacgagat caaggttaga aacggcttca tccagaatca
     5161 gtagtttcgg ttcgaccgcc agcgcgcgag ccaggcagac gcgctggagc tggccgccgc
     5221 ttaactgcgg tgggcgtttg tcgagaacgc tgtcatcgag atcgaccgcc ttcagcattt
     5281 cgctggcgcg cgccagttgt tcggattttt tcagtgatag caggtggcgc atcggttcac
     5341 gcaggatctc gcgcacggtt ttgcgcggat tcacggcgct gatggagtcc tgaaatacca
     5401 tctgaatatc gcggcggaac gctttacgct gggcgcgatt gagtttcgcc agcggttcgc
     5461 cacgccagct aatattcccc tgcgcgggcg attctaaacc caccagcaac cgcgcgaggg
     5521 tacttttccc gcagccgctg cgccccagca gggcgacggt ttcgccgctt ttcagggtca
     5581 gggaaacgtt attcagcacc gcctgatgtt gatgttttcc gttaaatcca ccgtgggcat
     5641 agtgatggga aaggccggag acgttaagta aagtcatgat gccagctcca taccgtagag
     5701 ggcgagatga gcggaaacca ggctgcgcgt taccgcatgt ttgggggcgt taaacagcgt
     5761 ttctacatcg ccctgttcga caattttacc ttgtgacatc accgccacgt catccgccag
     5821 acgcgccaca acgcccatat catgggtgac cagcagcatt cccggcgctt gtttttgcat
     5881 aatgctttcc agcagatcga ggatgcgcgc ctgtgctacc acgtcgaggt cggtggtcgg
     5941 ttcatcggcg atgataaacg gtgattcaca cagcaccgcc atcgcaatca tcatgcgctg
     6001 caacatgccg ccgctcatct cgaacgggta cagcttcagc acgcgcgcgg cgttttccag
     6061 ccccaccgct tctatggcag cggtaagcgt agcgtcatcg gcgggtttcc ctaacgccag
     6121 gcaggtttcc cgcgcgtggg tgtgcatggt gtgtagcgga ttaaaggcgc tgcgcgggtt
     6181 ctgcatgatg gtggcaattt tgatgccgcg cagggcgcag ggataaaccg ctttgccatc
     6241 ggctaaaatt tcccccgccg tctggcgaac gccagcgggc aaaatgccca gcgtcgcggc
     6301 gcaggttaat gatttcccgc tgccgctacc gccgactaac gccagcacgc gcccgcgttg
     6361 cagggttaac gatacaccgt gtaccagcgg ctgcgcggcc tgtagcgcga tattacgtag
     6421 ttcaatctgt tgcggcatta gtgtgcgtgc tccgtcacca gatgaggatc cagatgatcg
     6481 cgcagtgcgt cacccaccag gttaaaggcc atcacgctga taaacagcgc cagccccggc
     6541 cagaacattt gcagcggctg ggtccagata tactggcgcg cgtcgttaat catcacgccc
     6601 cattcggcgg tcggcgcggt cacaccgagg ccaaggaaag acatccccgc gacgtgcagc
     6661 atcatatggc cgatatccag cgttgccagc accagcagcg aagggatcac cgcgcctgcc
     6721 agatgatcga caaacacccg cacatggccc gcaccggaaa gccgtgacgc cagcacaaac
     6781 tcgcgttggc gtagtgaaat caccaagctg cgcaccatgc gtgcatacca cgcccagtgc
     6841 gacagggcga tggcgataat tacgttggtc agcccggtgc cgagcacgcc aaccataaag
     6901 aacgacagaa tcgaggtcgg gaaggtcata aacatatcgg cgacgcgcat ggtggcctga
     6961 tcaacgcgcc cgccaatcaa cccggcgctg ccgccaataa ccagccctaa tgtcagcacc
     7021 agcagcaggc aggccattac cgaaccgagc gacacgcggg tcgctgccat cagccgcgag
     7081 aaaatatcgc gacctaagtg atcggtgccc agccagtgct gcgcatccgg cgaaagcagg
     7141 cgcgacggca aatcaatcgc ctgggggtca tacggcagcc accactggct ggtgagcgca
     7201 atcagcgcca gcagggcgat aatgatcagc gccaggcgta ccgaccagcg ggaagagagg
     7261 aaaaagttca cgcgtgcgct ccttcatgac gacgaatgcg cgggtccagc gcggcgttga
     7321 gcaaatcgac aatcaaatta cagaccacaa aaaccaccac catcatcagc gtaaagcact
     7381 gaatcaccgg atagtcacgg ttaaaaatcg ccgacaccgc atagcgcccg acgcccggcc
     7441 aggcaaagat gttttcgata atcatcgtcc cgccaatcag ttcgccgatg tgcatcccca
     7501 cggcggtgat catcggcagc gaggcattgc gcagaatatg gcggcgttcg gtctgtttgt
     7561 cgttcaggcc gcgcagacgc gcccaagtga cgtgacgctg accggcgacg tccagcatac
     7621 tggcgcgcag cagtcgcgcg ttaatcgcca gcgacataaa ggcaatggaa accgcaggca
     7681 aaatgatgtg ctgccagccg ccgtaaccca tcgcgggtag ccattgcaga tacaccgaaa
     7741 acgccatcac cagtaaaaac gccagccaga agttaggcat cgacacgcca agaaacgcga
     7801 tgaaacgtac ggcgaaatcc ggcagacggt cgcgatggcg cgccgcccag ataccgagcg
     7861 gtacggaagt gagcagaatt aataccagcg ccgcacctgc aagttccagc gtggcgggca
     7921 ggaagttcag catatcgtcc agcaccgggc gttggctggc gaatgagata ccaaagtcaa
     7981 gatgcagcgc cttccacaac caggtaccgt actggacgta cagcggctga tccagcccga
     8041 gcatggtgcg ggtagaggcc agcatctccg gcgtcggcgg caggttagat aaacgcagat
     8101 aatcgagcgc cgggtcgccg gtaccgaggc gcagcatcag aaaaatgatc accgaggcgg
     8161 cgagcaccat cgggatcagc agcagaaagc ggcgtaatac gtaacgcaac attaaggttt
     8221 caccggttta atctgttcga acggaatttc ggtggcgatc ggcgcgtagg ggatattacc
     8281 cagctccggt tttgatacca ccatcatcga gatgtagctg ataggcagat aaaccgcctc
     8341 gtcatgcaga cgggtcaaaa tgtcgcgata cagcgcctga cgttgcgttt cgtcatgggt
     8401 cgccagcact tcgccgatct ctttatcaat cagcggcttg tcggctaatc cttgctgtgc
     8461 ctggaagtca gcgtgcgacg gtacgcgcat tgaactcagg aaggcgtgtg gatcgtatgg
     8521 cgcgccccag gtgcggtgga aaatcatgcc aaaacgaccg tcgcgctgac gagcatagat
     8581 actgctctct tcttcgccaa tcagcgagac atctgcgcca atctggcgca tatcagcctg
     8641 aatgatttcc gccatcgatt tgcttaacgc atcggtgccg atgaacgaca gttcaatgcg
     8701 cagcggctga ccatttttct cgcggatgtc tttgcccgca ggcagcgtcc aaccggcttt
     8761 ttccagcaac tctttcgctt tctgcggatc gtactggcgc ggtttcaggc cgagattagc
     8821 gtaaggcaca gaaggggcaa acagggtgtc ggcgacctgc ggggtgccat ataacgcgtt
     8881 atcaatcagc gattttttgt ttaccgcgta attaagagct tcacgtactg ccagctcgtt
     8941 ggtgggggct ttggcggtat tgagcgccag catcacggtt tcgatcggct gtgacagttg
     9001 ggtgtggtaa gccggattct ggctaaagcg ggcgaaggta tcgagcggta ataacccttc
     9061 gtttccgtac agcaggtcga tatcgccagt ttcaaacgcc accgcgcggg tagtcgggtc
     9121 cgggatgacg ttaaaggtga tctttttaat cgctggcttt tcgccccagt agttttcgtt
     9181 acggacgaag acatcgtact gattcagttt cgattcctgc aaaatccacg gtccggtgcc
     9241 aatcggcgct ttaattccgt tcatggtttc atggttttta aactgcgagg gagcgataaa
     9301 acggaaagga cggggcaggg ccagttcttg caggaaagga tagtaggcgc ttttcagggt
     9361 aatttgcagc tctgttttac tgagtgcttt aacatcaaca atctggtttg ccagctccag
     9421 ccaggcgtga cgttgacggt tatcgagcac tgcgcggaag ttttctgccg ccgcctcggc
     9481 atcgaacggt tcaccgttgg agaatttcac gtcatcacgc agggtgaagg tccaggtttt
     9541 accatcttct gaatgagtcc agctttttgc cagccacggg atcaccgaac cgtctgcctg
     9601 atatttcacc aatggttcat aaaccatgct ctgggcgaac atctggttag gcgtgtaaag
     9661 gtgcgggttt agtggcccga cattcaccgg ccaggcggta gtgatttcat ctggtgcagc
     9721 ggcatggacg ataaaagacg cacaagccag cagcgcaaat agagtgcggc ggagtgtgga
     9781 gagcatggta accccaatgg attaaaatag atggcggaaa taagtatgac gattttaagt
     9841 attcgtcata ctgattacct gttctggatc aatagatggg cagtcgggtg ggcggatcag
     9901 ttaactgaat cgatccattg cacactgtcg gcggtgagcg taaagggggt gggggtgcag
     9961 atcgcaaggc ttaaattttc tagctgacaa tggctgactg acagcgagga gtgataggtg
    10021 ctatctacgc tgacgatttg ccaggcgctg ccgccacgct gtttaacgat ggcttctttg
    10081 cgcgtccaga tgcgccagaa catttccagt tgctgatcag gatgcacggc gtccatttca
    10141 gcgtgttccc cgaggctgaa cacggcgttc gccagccagc gccagttggc gcgcgggcga
    10201 atcacttcga tatcgcagcc gacttcgcct tcatcgctca acagcagagc gatatcgtca
    10261 ccgctatggc ttaagttgaa ccatagcggc gtttccggcg cgaatgcagg tttgccttgt
    10321 tcgccataga tgatctccgg tagcggggaa agcgtgtgcg aaagcaatgc acgccccgcc
    10381 agccagcgtt cgcgtcgtgg accttgcggt gcttgctcgc gtaaacccgg tggcagtgga
    10441 gctgcgctta aggtcgaaac tttccccaga actatccgat acatatcagg gccaacgttt
    10501 aatggaaaat gaaagtgcgt atcgtatcac ttgtcgcctc atcccggtaa ccgacttttc
    10561 ggtctgcctg gccccagtaa aatcgccagt ttgctaccgc ctttggtggt ttccatccag
    10621 attttacaca cactggttaa tggcaccgaa agtagcatcc ctaccgggcc gagcagccat
    10681 ccccaaatta acaatgaaag aaataccacc atggtggaca tccccaggcg atggcccatc
    10741 atccgtggtt ctaaaatatt gccgatgacc atatggacca ctaaaaacaa tgcgccgacc
    10801 agaatacatt cgtaaacacc attaaacagc agcacctgaa tcattggcgg tacggcggaa
    10861 attaccgcgc cgatattggg cacgtagttg agcaaaaacg ccagtactgc ccacatcagc
    10921 gcaaactgca cccccatcag ctccagcccc agccagacga tgacacctgt ccataaactg
    10981 agtagcgtct tcaatgcaag atagtgcgaa acgcctttaa gtgcgcggtg taatcctgcg
    11041 atgtgaatct gtgggttatt cagcgcaaaa cgcattttgt aagggacgtg gcgcacttca
    11101 aacagcataa aaactacggt catcaccagc aaaagcacgc tcgccattgc cccggaaagc
    11161 ccggtcatta gcgctgtggt gaaggtaacc actttttccg agtccatccg ctgcagcatt
    11221 cgctccggcg acatatgcaa attaagaaaa ggcaacatct cctgcaattt aaaaagtttg
    11281 cgcgtcagct ccttattaaa cttcggcagc atagagataa attcgttaaa cgatgccgcc
    11341 agtacgccga ccagcgcggt tagtgcgatc agcatcacca ccactacaat cgtaatggca
    11401 acggggcgtt gtactccccg acgaataaac caggtaacga gcgggttgag gacgatggca
    11461 aaaaacagcg ccagtaacag ctgcacaatg atatctgccg ctgcgtgaat gcccgcgagg
    11521 atcactacca gcgaggccag cttgagcaga atgtgcatgc ccgttttatc gggttgaggg
    11581 atttccattg gggcttcctt gtgacttttt gtattaagtg tagcgggagt cacgccagca
    11641 atattctgat tcttactgct gattttcacg tcagcacgtg gtaaaaatga aacactgttg
    11701 taaaaatgtg gtgatcctca tgcccgaacc cgtagccgaa cccgcgctaa acggattgcg
    11761 cctgaatttg cgcattgtct ccattgtcat gtttaacttc gccagctacc tcaccatcgg
    11821 gttgccgctc gctgtattac cgggctatgt ccatgatgtg atgggcttta gcgctttctg
    11881 ggcaggattg gttatcagcc tgcaatattt cgccaccttg ctgagccgtc ctcatgccgg
    11941 acgttacgcc gatttgctgg gccccaaaaa gattgtcgtc ttcggtttat gcggctgctt
    12001 tttgagcggt ctgggatatc tgacggcagg attaaccgcc agtctgcccg tcatcagcct
    12061 gttattactt tgcctgggac gcgtgatcct tgggattggg caaagttttg ccggaacggg
    12121 atcgaccctg tggggcgttg gcgtggttgg ctcgctgcat atcgggcgag tgatttcgtg
    12181 gaacggcatt gtcaattacg gggcgatggc gatgggtgcg ccgttaggcg tcgtgtttta
    12241 tcactggggc ggcttgcagg cgttagcgtt aatcattatg ggcgtggcgc tggtggccat
    12301 tttgttggcg atcccgcgtc cgacggtaaa agccagtaaa ggcaaaccac tgccgtttcg
    12361 cgcggtgctt gggcgcgtct ggctgtacgg tatggcactg gcactggctt ctgccggatt
    12421 tggcgtcatc gccaccttta tcacgctgtt ttatgacgct aaaggttggg acggtgcggc
    12481 tttcgcgctg acgctgttta gctgtgcgtt tgtcggtacg cgtttgttat tccctaacgg
    12541 cattaaccgt atcggcggct taaacgtggc gatgatttgc tttagcgttg agataatcgg
    12601 cctgctactg gttggcgtgg cgactatgcc gtggatggcg aaagtcggcg tcttactggc
    12661 gggggcaggg ttttcgctgg tgttcccggc attgggcgta gtggcggtaa aagcggttcc
    12721 gcagcaaaat cagggggcgg cgctggcaac ttacaccgta tttatggatt tatcgcttgg
    12781 cgtgaccgga ccactggctg ggctggtgat gagctgggcg ggcgtaccgg tgatttatct
    12841 ggcggcggcg ggactggtcg caatcgcgtt attactgacg tggcgattaa aaaaacggcc
    12901 tccggaacac gtccctgagg ccgcctcatc atcttaaaac ttactgaata accagcgtat
    12961 taatgatgtt ttctgcggtg gtctgcgctt tttgctgatc gtcagctggc agagtaattt
    13021 gcatggtcag cagttgatta cccacgttac ccagaataac ggaagagtac gccgtctggc
    13081 ctttcgcgga gataatactg tctaactgct gcattttgtg acctttcagc tcaatggctt
    13141 tgttggttac cacttgcagc tgcggatcgc ggctacgttg ctgatcttcc agacgcttcg
    13201 ccaacaccgc cagatcttct ttcggatcat cgcccatgat gacgatgact gctttctgcc
    13261 cggtggcgtc ggaccagaca tgcatgttat tggcctgcgt tcccagctta ccgctctggt
    13321 cggtcatatc cgctggcagc gagaaactta acttgccatc aagcaggttg acgggattcc
    13381 cggtagcgtt actttccgcg accgaaccct gcgccgtagc gttagtgtct ttatcatcac
    13441 aggccgcaag ccccataacc agcaggccaa ttccgacata tttaaccaga ttgcgcattg
    13501 acttcttcct ttcgataaac ggccataacg gctcattcat ccatcttatc acaactctga
    13561 taacgaacct ttaactcgcc tgcaaagcgt tgatttcgga tttatctgcc agtcttttca
    13621 acagaatatt gagtaatacg ccatacattg gcaggaagaa aacgatactg attaacactt
    13681 tgaaacagta atcgaccagc gcgatttcca tccagtgttc agccataaag gcatccgggc
    13741 tacgccagaa ggcaatgaag aaaaaggcca gcgtgtcgct gacgttaccg aacagtgtgg
    13801 acgctgtcgg tgccagccac cagcggcgac tctgacgcag gcggttaaaa acgtgcacgt
    13861 cgaggatctg tcccagcgcg taggccatga aactggcggt ggcgatacgg gcgacaaaca
    13921 ggttgaagtg ggcgagtgcg ccgaatccct gccaggaacc catatagaat agcgacgaga
    13981 tgacgtagga gatcagcaac gccgggatca tcaccgcaaa gatgatacga cgggccagcg
    14041 gtgcgccaaa aatacgcacg gtcaggtcgg tagcaaggaa aataaacggg aagctaaacg
    14101 cgccccaggt ggtatggaaa cccaaaatgg agacgggaag ctgaaccaga tagttactgg
    14161 aggtgatcac cagcagatga aataacgata accagaacaa cgccttatag cgttgagttt
    14221 gcgagaaaac gttcatattg tacctttttg attaaccatt ggggtgaggg aacccaatac
    14281 gtacgacacg tctgttacct taacataata acgtgcttac ccttttttcg agccgccgca
    14341 tgatactgct ttacgttgac aatgcaatgg ttaattttca cgcaatcgtt aacctggttt
    14401 gcttacggac tcacagggcg taaactagcg ccgttttttt atgtgatgag aagaaaatga
    14461 ccgatctctt ttccagccct gaccacacac tcgacgcact tggcctgcgc tgcccggaac
    14521 ccgtaatgat ggtgcgcaaa actgtgcgca atatgcagcc tggcgaaacg ttgctgatta
    14581 tcgccgacga tccggccact acccgcgata ttcctgggtt ttgtaccttt atggaacacg
    14641 aactggttgc gaaagagacg gatggactgc cttatcgtta tttgattcgt aaaggcggtt
    14701 gatggggaaa gattggcttc gatgccgcct tttcccctca ccctaaccct ctccccagag
    14761 gggcgagggg accgatcgcg ctcaatgttg cgatcggttt gccttatctc ctgcgcaaca
    14821 atcttaacgc attcgctgtc accagcaccg tcgcacccgt atctgccagc accgccagcc
    14881 acaggccggt catccctaac agcgtggtga cgaggaagat ccctttcagc cccagcgcaa
    14941 tggtgatgtt ctggcggata ttggcgtgag tggcgcgtgc cagttcaatc atttgcacca
    15001 ggccgcgcag gtggttatgg gttaatgctg catcagcggt ttccagcgcc acgtctgtgc
    15061 cactgcccat tgcaatccca atggcggcag ctttcatcgc tggcgcgtcg ttaataccat
    15121 caccgaccat cgccaggggc gcatgttgat tcagcttggt caccgctttg actttatctt
    15181 ccggcaatag gcccgcttta aactccagcc ccagctcccc ggcgattgcc gccgctgcac
    15241 gtggattatc gccggtgagg atcactcctt tgacccccag cgcgttcagt tcactgatgg
    15301 cagttgcagc atcggcgcgc agggtatcct gcaatgcaat gatacccagc acgtcatcat
    15361 tacgtactac cagcacaacc gtttgcccgg cgctttccag ttcattaatc aaaccagcaa
    15421 atgcatcagc gggatgtttc ccggcagcgc aaatcaatac gcgctcaccg ttaacctgcg
    15481 cttcaatgcc agacccgacc agcgcccgct gtgattcggc ggtgggaatg gcgagttcag
    15541 caacctgtgc ttcgcgtacg atggcttgcg ccagtggatg cgtcgcgcct tgctcgaccg
    15601 ccgccgccag tgtcagcagt tcagattcac taatacccgt tgccggatga atcgcggtaa
    15661 cgcgcggttt accgacggtc agcgtaccgg ttttatcaaa cgccacctga gtaacacgac
    15721 ctagttgttc cagcgccgct ccgcctttaa tcaacgcccc acgacgcgct gccgccgcca
    15781 gcccggaggt aatcgccgca ggcgttgaga taactaacgc acacgggcag ccaatcagca
    15841 gcagcgtcag ccctttataa atccactcct gccagctggc ggcaaacagc agcggtggca
    15901 ccagcgtcac cagcagagcg acggccataa tcgcgggcgt atagatacgg ctgaaacggt
    15961 cgataaaccg ctcaatgggg gcgcgacgct cttcggcttc ttcgatcagt ttcagaatcc
    16021 ggtcaatggc gctggcaccc ggttctgaca gcacttccag cgttaccaga cggtctacgc
    16081 tggtggctcc tgcaggaact ttatcgcccg tcgcgcgctc caccggaatg gattctccgg
    16141 tcagggcgct ttcatcaaaa ctggcaaacg gtgagagcag tttaccgtcg gcaggcaaac
    16201 gcccacctgc ggcgacttca atcacatcgc caggccgcag gctgttaatc gccacctctt
    16261 cccgctcacc gttacgcagg cgcgtggcgg tttctggttt cagcgccatt aacgcgctaa
    16321 cgccctgacg cgcgcggctg gcggcccagc cttccagtcg ttcaccaatc aaaaacagca
    16381 gcaacaccat cgcagcttca gccgttgcgc caataaacag tgcaccaata gcggctacgc
    16441 tcattaaggt ttcaatggcg aagtagctgc cggatttgat cagccgtaat gcctgacgag
    16501 caatcgggta cagcccaacc agcgtggtcg cgataaacgc cagttgcccg aacggatgat
    16561 tgaactgctc cagaccccag ctgattgcca tcatcacgat tagtgtaatc agcggcagat
    16621 tctctttcag gcgcgatgct tgtggctcgt cggcggcctg ctcatcgcgc agggaatagc
    16681 ccgctttttg caccgcagat tcaacttgtg cacgaatgtc attgtcggca tcaaccacca
    16741 gtttttcggt ggcgaacaac acctgcacct gattcacacc tgcaagctgg cgcacggcat
    16801 tttctacctt gcgcgcacag gcggcgcagt ccatgccact gactttccag ctatagcggg
    16861 tgccggagac gttttcagag agcgttggcg agctggagca tgcgccgtcg cagcaacagt
    16921 cgttggcgtt ctgtaccgtg gttagcggtt tgaacgcagc aaattgaggg gctttcttgc
    16981 cgtgattgtc aggtgtcgac atggcatact ccggttaagt tttttctcat taaccgaagg
    17041 atacactctg gagtcgactc cagagtcaag ttttatcaga gatacagtga gcggatgatg
    17101 aggaagtgac cggcaaagta gcaggccgca gcaatcgcgt tatccgcacg gaagcgtcgg
    17161 cgatagtggc tccccagcca gacaaagtta ctgataaaca gcaacgaagc gccaacaaac
    17221 gcagagagcg ccggagcggt cggacggaag aaccacagtt cacctgccag ccacaccatc
    17281 accagcgtca tgccgataaa cgtgcagata ggccaacgat actcttccag ccgcgtccag
    17341 ataatcgcca gtaacagcgc acccagaacc agcaacacca gtggcagagg ccagaagaaa
    17401 gagagcgtca tctgactggc gaaatagatg gtgtacagca ggtgcgagag gaaaaacgcg
    17461 ccgatggcgt acatcagacg ttgacgtggc aacagggtta gcgcatcgcc cagcagtgag
    17521 gcgcacagcc ctgccagcac cagatagcta atggcgtcga acatcggcgc ttgccaggcc
    17581 agtaacagca ggagaagaag ggttaacggt ttaaacaccc agcgttgcca ggttggccca
    17641 cgatacgatg catccacaga tagccatgcg gaaagacaga cagcgataaa cgaccaaagc
    17701 atcttagttc cttgaattcg ttattttttc gtaagcggaa tgcctacggt cttgcttcca
    17761 gtttagtggc gagggtggac ggatgacaac accataactg gcagggtatg cttatttccg
    17821 cattttccga tgagggataa agatgagcaa accacctctt ttctttattg ttatcattgg
    17881 cttaattgtc gtcgccgcat cgtttcgttt tatgcaacag cgacgggaaa aagctgataa
    17941 tgatatggct ccgctccagc aaaagctggt ggtggtgagc aacaagcggg aaaaaccgat
    18001 taacgatcgc cgttcgcgcc agcaggaagt gactccggca ggcaccagta tgcgctacga
    18061 ggcaagcttc aaaccgcaaa gcggaggaat ggagcagacg tttcgcctcg acgcccagca
    18121 gtaccacgcc ctgacagtgg gcgataaagg tacgctgagc tataaaggaa cgcgctttgt
    18181 tagctttgtt ggcgaacaat aaggttattt tttaacttta aatttcttct gccagaccag
    18241 cagttcaaac acgccgaaaa ggaaaatccg taccttttcg gcggtggtca tctgcgggcc
    18301 atctttcggt aaagtggatt tcaatagcgc cagctgcata ccatgcatca gcacggtaaa
    18361 aatcagcgcc acgttaacga aaatatttag cgggcgcggg aagggatgca ccagattgag
    18421 gattaaaaat ccccaaacgc agagcattaa caaacgacca atattaatca gcatcacttt
    18481 ctccttgtgc ttcgcgttga tacagccgat aagccacctg acccgccact ttttcccgat
    18541 gtaacgacca gtttgccgga acggtgggca gaccgttttc gacttcgctt tcgacataaa
    18601 tcagggcttc gtcagccagc cagccgttat cttccagtaa atttatcgtc tcttctaaca
    18661 agccacggcg gaacggtgga tcgacaaaca cgatattatg cggtgtgcct ttttgcgcca
    18721 ggaatgacat cgcgttgctg ttcaccacgc gtgcattgcc tgcttttagt gtcgccagat
    18781 tcttaattaa ctgctgagaa actgcacgat ccatctcaat taacgtggcc cccgcagcgt
    18841 agcgcgataa cgcttccagc cccagcgcgc cgctcccggc gaagcaatcc agacattggg
    18901 cgtcaacaat gaccggagcc agccagttaa acaacgtttc gcgtacgcgg tcggtggtgg
    18961 ggcgcagacc tgggctatca ggaaccggga gtttacggcc tcgccactgc ccgccaataa
    19021 tgcggatttg gccgctgccg gaatgattcg gttttttcat gataaattgc tcaatccgcc
    19081 agataacaca tacttgcagg cggtgatgtg aattaagtta agtgatagac tatttcatca
    19141 tttttttagc tgctatgtac atagcgttaa cgctgtgcca tgaagcaaca gcgaggagtg
    19201 tagtcgcaaa tggcgaaaga aaaaaaacgt ggcttttttt cctggctggg ctttggtcaa
    19261 aaagagcaga ccccggaaaa agagacagaa gttcagaatg aacaaccggt tgtagaagaa
    19321 atcgttcagg cgcaagagcc tgtgaaggct tcggaacaag ccgttgaaga gcagccgcag
    19381 gcgcatactg aagccgaggc ggaaactttt gctgccgacg ttgtggaagt cactgaacag
    19441 gttgttgaaa gtgaaaaagc gcagcctgaa gcggaagttg ttgcacagcc ggaaccggtc
    19501 gtagaagaaa cgccggagcc agtggctatc gaacgtgaag agctgccgtt gccggaagac
    19561 gtcaacgccg aagaggtttc gccagaagag tggcaggctg aagcggaaac cgtagagatt
    19621 gtcaaagcgg cggaagaaga agcggctaaa gaagaaatca ccgacgaaga gctggaagca
    19681 caggcgctgg cagccgaagc agcagaagag gcggtgatgg tggttcctcc ggcagaagaa
    19741 gagcagccgg tggaagaaat cgctcaggag caggaaaaac caaccaaaga aggtttcttc
    19801 gcgcgcctga aacgcagcct gttaaaaacc aaagaaaatc tcggttccgg atttatcagc
    19861 ctgttccgcg gtaaaaaaat cgacgatgat ctgtttgagg agctggaaga gcagcttttg
    19921 atcgccgatg tgggcgtgga aaccacacgt aaaattatca ccaatctgac ggaaggcgca
    19981 tcccgcaagc agcttcgtga tgccgaggcg ctctatggcc tgctgaaaga agagatgggt
    20041 gagattctgg cgaaagtcga tgagccgctg aatgttgaag gtaaaacgcc gttcgtgatc
    20101 ctgatggtgg gcgtcaacgg tgtgggtaaa accacgacga ttggtaagct ggcacgtcag
    20161 tttgagcagc agggtaaatc ggtgatgctg gcggcgggcg atactttccg tgcagcagcg
    20221 gttgaacagc ttcaggtctg gggtcagcgc aacaatattc cggtgattgc ccagcatacc
    20281 ggggcggatt ccgcctctgt tatcttcgac gccattcagg cagctaaagc gcgtaatatc
    20341 gacgtcctga ttgccgatac cgccggacgc ctgcagaaca aatcgcacct gatggaagag
    20401 ttgaagaaaa tcgtccgcgt gatgaagaaa ctcgacgttg aagcgccgca tgaagttatg
    20461 ctgactattg atgccagcac cgggcagaac gcggtaagcc aggccaaact gttccacgaa
    20521 gctgttggct taaccggcat cacgctaacg aaactggacg gcacggcgaa aggcggggta
    20581 attttctcgg tggctgacca gtttggtatc cctatccgct acattggtgt cggcgaacgt
    20641 attgaggatt tgcgtccgtt taaggcggac gactttatag aggcactttt tgcccgagag
    20701 gattaacaat gattcgcttt gaacatgtca gcaaggctta tctcggtggg agacaggcgc
    20761 tgcagggcgt tacgttccat atgcagccgg gtgagatggc gtttctgacc ggtcattccg
    20821 gcgcagggaa aagtaccctc ctgaagctga tctgtgggat tgagcggccc agcgccggga
    20881 aaatctggtt tagcggccat gacatcacgc gtctgaaaaa ccgtgaagtt ccgtttctgc
    20941 gccgccagat tggcatgatt ttccaggatc accatctact gatggaccgt actgtctacg
    21001 ataacgtggc gatcccgctg attatcgctg gtgccagcgg tgacgatatt cgtcgccggg
    21061 tgtcggcggc actggataaa gtcgggctac tggacaaagc gaagaacttc cctattcagc
    21121 tttccggcgg tgaacaacag cgtgttggca ttgcccgcgc ggtggtgaat aagcccgcgg
    21181 tactgctggc ggacgaaccg accggtaacc tggacgacgc gttgtcggaa gggattctac
    21241 gtctgtttga agagtttaac cgcgttgggg taaccgtatt gatggcaacg cacgacatca
    21301 acctgatttc acggcgttcc catcgcatgc tcaccctgag cgatggtcac ttgcatggag
    21361 gcgtgggcca tgaataagcg cgatgcaatc aatcatattc ggcagtttgg tgggcgtctg
    21421 gatcgcttcc gtaaatcggt cggcggctca ggcgacggcg gtcgtaacgc accgaaacgc
    21481 gcgaaatcct cgccaaaacc ggtaaatcgc aaaaccaacg ttttcaacga acaggtgcgc
    21541 tatgccttcc acggcgcatt gcaggatctg aaaagcaaac cgttcgccac gtttttaacg
    21601 gtgatggtta tcgccatctc cctgacgctg cccagcgtct gttatatggt gtacaaaaac
    21661 gttaatcagg cggcgacgca gtattatccg tcaccgcaaa tcactgttta cttgcaaaaa
    21721 acgctggacg atgacgccgc tgcgggcgtg gtggcacagt tgcaggccga gcaaggcgtg
    21781 gagaaagtga actatctttc tcgtgaagac gcactgggcg agttccgtaa ctggtctggt
    21841 tttggtggtg cgctggatat gctggaagaa aacccgcttc cggcagtggc ggtggtgatc
    21901 ccgaaactcg attttcaggg gacggaatca ctcaatacgc tgcgtgatcg tatcacgcag
    21961 attaacggca ttgacgaagt gcggatggat gacagctggt ttgcccgtct ggcggcgttg
    22021 accgggctgg tcgggcgcgt ttcggcgatg atcggcgtgt tgatggtggc ggcagtgttc
    22081 ctcgtcatcg gcaacagcgt acgtctgagc atctttgctc gccgtgactc catcaacgtg
    22141 cagaaactga ttggtgcgac ggatggtttc atccttcgcc cgttcctgta tggtggcgca
    22201 ctgctgggat tttctggcgc attgttgtca ttaattttgt cagaaattct ggtgctgcga
    22261 ttgtcatcgg cggttgcgga agtggcacag gttttcggaa cgaagtttga tatcaatgat
    22321 ttatcgttcg atgaatgcct gctattgctg ctggtatgct cgatgattgg ctgggtggca
    22381 gcgtggcttg ccacggtaca acatttacgc cactttacgc ctgaataata aaagcgtgtt
    22441 atactctttc cctgcaatgg gttccgtagc agggaaagag atcccgttgt ctcttcccgg
    22501 tatttcatct ctatgtcaca ttttgtgcgt aatttattca caagcttgca ttgaacttgt
    22561 ggataaaatc actgtctgat aaaacagtgg atgataacct cgttgctctt aagctctggc
    22621 acagttgttg ctaccactga agcgccagaa gatatcgatt gagaggattt gaatgactga
    22681 caaaatgcaa agtttagctt tagccccagt tggcaacctg gattcctaca tccgggcagc
    22741 taacgcgtgg ccgatgttgt cggctgacga ggagcgggcg ctggctgaaa agctgcatta
    22801 ccatggcgat ctggaagcag ctaaaacgct gatcctgtct cacctgcggt ttgttgttca
    22861 tattgctcgt aattatgcgg gctatggcct gccacaggcg gatttgattc aggaaggtaa
    22921 catcggcctg atgaaagcag tgcgccgttt caacccggaa gtgggtgtgc gcctggtctc
    22981 cttcgccgtt cactggatca aagcagagat ccacgaatac gttctgcgta actggcgtat
    23041 cgtcaaagtt gcgaccacca aagcgcagcg caaactgttc ttcaacctgc gtaaaaccaa
    23101 gcagcgtctg ggctggttta accaggatga agtcgaaatg gtggcccgtg aactgggcgt
    23161 aaccagcaaa gacgttcgtg agatggaatc acgtatggcg gcacaggaca tgacctttga
    23221 cctgtcttcc gacgacgatt ccgacagcca gccgatggca ccggtgctct atctgcagga
    23281 taaatcatct aactttgccg acggcatcga agatgataac tgggaagagc aggcggcaaa
    23341 ccgtctgacc gacgcgatgc aaggtctgga cgaacgcagc caggacatca tccgcgcgcg
    23401 ctggctggac gaagacaaca agtccacgtt gcaggaactg gctgaccgtt acggtgtttc
    23461 cgctgaacgt gtacgccagc tggaaaagaa cgcgatgaaa aaactgcgcg ctgctataga
    23521 agcgtaattt ccgctattaa gcagagaacc ctggatgcga gtccggggtt tttgtttttt
    23581 gggcctctac aataatcaat ttcccctccg gcaaaacgcc aatccccacg cagattgtta
    23641 ataaactgtc aaaatagcta ttccaatatc ataaaaatcg ggtatgtttt agcagagtat
    23701 gctgctaaag cacgggtagc catgcataaa acgaaataaa gtgctgaaca acaacatcac
    23761 aacacacgta ataaccagaa gaatggggat tctcaggatg aacataaagg gtaaagcgtt
    23821 actggcagga tgtatcgcgc tggcattcag caatatggct ctggcagaag atattaaagt
    23881 cgcggtcgtg ggcgcaatgt ccggtccggt tgcgcagtac ggtgaccaag agtttaccgg
    23941 cgcagagcag gcggttgcgg atatcaacgc taaaggcggc attaaaggca acaaactgca
    24001 aatcgtaaaa tatgacgatg cctgtgaccc gaaacaggcg gttgcggtgg cgaacaaagt
    24061 cgttaacgac ggcattaaat atgtgattgg tcacctctgt tcttcatcaa cgcagcctgc
    24121 gtctgacatc tacgaagacg aaggcatttt aatgatcacc ccagcggcaa ccgcgccgga
    24181 gctgaccgcc c
//
LOCUS       DAJTZL010000047        24126 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.047, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000047 DAJTZL010000000
VERSION     DAJTZL010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24126
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.047"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            71..223
                     /gene="hokE"
                     /locus_tag="OO544_001673"
     CDS             71..223
                     /gene="hokE"
                     /locus_tag="OO544_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HCR6767292.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            complement(345..965)
                     /gene="entD"
                     /locus_tag="OO544_001674"
     CDS             complement(345..965)
                     /gene="entD"
                     /locus_tag="OO544_001674"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HCR6767293.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAVYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGTTALAVVSRQ
                     PIGIDIEEIFSVQTARELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASER
                     QTEAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(1140..3380)
                     /gene="fepA"
                     /locus_tag="OO544_001675"
     CDS             complement(1140..3380)
                     /gene="fepA"
                     /locus_tag="OO544_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HCR6767294.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDSYTRSKYGDETNRLYRQNYSLTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGANTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYQQGNDDLKAETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSEMVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SVNTHF"
     gene            3623..4825
                     /gene="fes"
                     /locus_tag="OO544_001676"
     CDS             3623..4825
                     /gene="fes"
                     /locus_tag="OO544_001676"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HCR6767295.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWIYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQAPLQPGWDCPQV
                     PEIAAKEIIWKSERLKNSRRVWIFTTGDVTAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKAIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRDGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            4828..5046
                     /gene="ybdZ"
                     /locus_tag="OO544_001677"
     CDS             4828..5046
                     /gene="ybdZ"
                     /locus_tag="OO544_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HCR6767296.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCLLSAGWDIVCQPQ
                     SQASCQQWLEVHWRTLTPANFTQLQEAQ"
     gene            5043..8924
                     /gene="entF"
                     /locus_tag="OO544_001678"
     CDS             5043..8924
                     /gene="entF"
                     /locus_tag="OO544_001678"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HCR6767297.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGDVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDALIFEQPEIIDLRTNIDPHGTAQALML
                     ADLQQDLRVDSGKPLVFHQLIHVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCTW
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLPGR
                     SASADILRLKLEFTDGEFRQLATQLSGVQRTDLALALAALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTASGPVLNVLPLGIHIAAQETLPELATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGDEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHGDLSI
                     EILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPDAPALADARYLFSYREMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     SDVPNLTSLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQSCATLKQVFCSGEALPADLCREWQQLTGA
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNMGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKTQASGRAP
                     KAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTPGQVM
                     VASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DPQWSIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAEL
                     DIYRQDCAHVDIISPGAFVKIGPIIRATLNR"
     gene            9140..10273
                     /gene="fepE"
                     /locus_tag="OO544_001679"
     CDS             9140..10273
                     /gene="fepE"
                     /locus_tag="OO544_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HCR6767298.1"
                     /translation="MSSLNIKQGSDAHFSEYPLASPSNNEIDLLNLIEVLWRAKKTVM
                     AVVFAFACAGLLISFILPQKWTSSAVITPAEAIQWQDLEKTFTKLRVLDLDVNIDRGG
                     AFNLFIKKFQSVSLLEEYLRSSPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDEPSLYTSWTLSFTAPTSKEAQTVLSGYIDYISALVVKESIENVRNKLEIKTQFE
                     KEKLAQDRIKTKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKANVNDVNFTPFKYQLSPSLPVKKD
                     GPGKAIIVILSALIGGMVACGSVLLRYAMASRKQDAMMADHLV"
     gene            complement(10270..11085)
                     /gene="fepC"
                     /locus_tag="OO544_001680"
     CDS             complement(10270..11085)
                     /gene="fepC"
                     /locus_tag="OO544_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HCR6767299.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATRITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTAPSTANS"
     gene            complement(11082..12074)
                     /gene="fepG"
                     /locus_tag="OO544_001681"
     CDS             complement(11082..12074)
                     /gene="fepG"
                     /locus_tag="OO544_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HCR6767300.1"
                     /translation="MIYVSRRLLITCLLLISACVVAGIWGLRSGAVTLEISQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIVTSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAAALLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            complement(12071..13075)
                     /gene="fepD"
                     /locus_tag="OO544_001682"
     CDS             complement(12071..13075)
                     /gene="fepD"
                     /locus_tag="OO544_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HCR6767301.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFIGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            13186..14436
                     /gene="entS"
                     /locus_tag="OO544_001683"
     CDS             13186..14436
                     /gene="entS"
                     /locus_tag="OO544_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HCR6767302.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDS"
     gene            complement(14517..15482)
                     /gene="fepB"
                     /locus_tag="OO544_001684"
     CDS             complement(14517..15482)
                     /gene="fepB"
                     /locus_tag="OO544_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HCR6767303.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAVVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYTLGTETFRLDYYSAMQVLDRLNSLLKKD"
     gene            15857..17032
                     /gene="entC"
                     /locus_tag="OO544_001685"
     CDS             15857..17032
                     /gene="entC"
                     /locus_tag="OO544_001685"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HCR6767304.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QTSAHCFTRSQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            17042..18652
                     /gene="entE"
                     /locus_tag="OO544_001686"
     CDS             17042..18652
                     /gene="entE"
                     /locus_tag="OO544_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HCR6767305.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVTEHSSIRVVQLLNDSGE
                     HNLQDAINHPADGFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIAKHQVNVT
                     ALVPPAVSLWLQALAEGESRAQLASLKLLQVGGARLSATLAARISAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            18666..19523
                     /gene="entB"
                     /locus_tag="OO544_001687"
     CDS             18666..19523
                     /gene="entB"
                     /locus_tag="OO544_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HCR6767306.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEGLLPAPVPARKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            19523..20269
                     /gene="entA"
                     /locus_tag="OO544_001688"
     CDS             19523..20269
                     /gene="entA"
                     /locus_tag="OO544_001688"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HCR6767307.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFAQEQY
                     PFATEVMDVADAAQVAQVCQRLLAETERLDVLVNAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAVSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            20272..20685
                     /gene="entH"
                     /locus_tag="OO544_001689"
     CDS             20272..20685
                     /gene="entH"
                     /locus_tag="OO544_001689"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HCR6767308.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPMSEGKVRGVC
                     QPLHLGRQNQSWEIIVFDEQGRRCCTCRLGTAVLG"
     gene            20866..22971
                     /gene="cstA"
                     /locus_tag="OO544_001690"
     CDS             20866..22971
                     /gene="cstA"
                     /locus_tag="OO544_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein CstA"
                     /protein_id="HCR6767309.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLAIFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFAITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKSALAALKDPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            23153..23350
                     /locus_tag="OO544_001691"
     CDS             23153..23350
                     /locus_tag="OO544_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HCR6767310.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(23360..>24126)
                     /gene="hcxA"
                     /locus_tag="OO544_001692"
     CDS             complement(23360..>24126)
                     /gene="hcxA"
                     /locus_tag="OO544_001692"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HCR6767311.1"
                     /translation="LGLPFVAVPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMV
                     LVEPEIILNAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLL
                     NSSEQALSDQQNQQLTQSFCDVVDAIIAGGGMVGDLGDRFTRVAAAHAVHNGLTVLPQ
                     TEKFLHGTKVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEMDINNHAEIDK
                     VITHTLRPVEYIHYLPVTLTPDTLRAAFEKVESFKA"
BASE COUNT         5272 a         6331 c         6836 g         5687 t
ORIGIN      
        1 cctggggctg ccactccgaa cctcaacaac tcggatggta gtcccttctt cgcatggagg
       61 caatataaac atgctgacga aatatgccct tgtggcagtc atagtgctgt gtttgacggt
      121 gctgggattt acgcttctgg tcggagactc gctgtgtgag ttcaccgtaa aggaacgtaa
      181 tattgagttt aaggctgttc tcgcttacga accgaagaag tagccgttgt gcggggagca
      241 atccctgcat atccggttgt caggtcagag tggtaaggca cctgctttac actttcgccc
      301 gcagtcagtg atggctgcgg gcgaatcgta ccagatgttg tcaattaatc gtgttggcac
      361 agagttatga ctattttttc ttttatctgc cagtgcacag caaacatctc attctcacga
      421 tgaatgatga cctgctgttt attccagcta attatctgat aatccagaaa acctgcttca
      481 gtttgtctct ccgatgcctt aaatgcgctc tctttggcgg aaaatgccag tgtcagcgcc
      541 agagaaaagg ctaaaccaca gtccgcgagt cgctcgtgtt ctgctggtgt aataatattg
      601 tctgtcaatt ctcttgcggt ttgtacagaa aaaatttctt ctatatcaat gccaatcggt
      661 tgacgagata ccacggctaa tgccgtagtc ccacagtggc taatactgcc gtatacctcc
      721 gcaggccaga caggttggcg tagctcgccg attgcgggca cacatttata gccatattcc
      781 cgtaaggcat aaacagcagc gatccgtccg gctaaatgct ctgttttacg tttacgtcca
      841 gcgtgttgca attgtgcgta gtgcggcagc cagagtaaat cctgctcaca aaaattcgcc
      901 ggatcgaact caacaaaatg cagcgtatgt ccggcaaagg ggagggaggt atgcgtagtt
      961 ttcatatcga ccatattcga gactgatgac aaacgcaaaa ctgcctgatg cgctacgctt
     1021 atcaggccta catggtcctg caatatattg aattggcaag atttttgtag gccggataag
     1081 gcgttcacgc cgcatccggc atgaacgacg cgcactttgt caacaatctg acgttagcat
     1141 cagaagtgag tgtttacgct catgtaccac gtacgtcccg gctcgttata ggtatacgcc
     1201 ccggcacccg cgatatagtt ggcccctgcc agatcgcccg tggtctgggc attacccgca
     1261 cgccacaaac gtttgtcgaa caggttgtcc acgccgccgg tcagactgac attcttcgtc
     1321 acatcccagg tcgcgctcag acccacaatg ctgtaaggac tgatttcttt ggtttccggg
     1381 ccaaccgctg gctgaccttt gtagttgtat tttttcggct gctgcttacc gtaccaggtg
     1441 aaggtcgttt gcatcgacaa atcttcccgc gcctgccagc tcagcgttga gttcaacgtg
     1501 tactccggga tgatcgacaa acggtcgccc gtggttttgt tttcactctt cagcatataa
     1561 gtgatgttat tggtccacat caccatttcg ctaactggta cgtttaacga tccttccaga
     1621 ccttcaacca ctgctttcgg cacgttatcc cactgataga gatcggtgcc gactgcgttt
     1681 tgccctacag ccacatagcc cgcttcaatc ttattgcgat aatcgttacg gaaccaggtg
     1741 acgcccgcca gccagccgtc gcgtttgaac tccagaccaa tctctttgtt gatgctggtt
     1801 tctgctttca ggtcatcgtt accttgctga tagcagccgc ccgcgctggc atagcagccc
     1861 tgacctttac tgtagagaat gtagttcggg ttagtctggt acaggctcgg tgctttataa
     1921 gcacgggcaa tgcccatttt gagcgtgaag tcatcgccta aaccttgcga tatgttcagc
     1981 gccgggctcc agttattgcc gacaatactg tgatgatcga aacgcagccc cggcgttacg
     2041 atggtgctgt cagtcagctc catgttgttt tcggcaaaca gcgagaaaat ttctgctttt
     2101 gaatacgggc tacggtcggt ggcactcaca ccatcaatag cgccgccggt gttcgccccg
     2161 gtcagcgcct gggtattgga actcaagtcc ttcatccgtt gctgattcca ctccgtaccc
     2221 agcgtcagcg tttggttaac gaggaaatca atcggcaggt taacttcgct gtgcagcatc
     2281 acgtcatcaa gatcgatatc gacgaaatcc tgtgtcgctt tttcgttaaa tttcccttcg
     2341 gtaccgcctg ccagaccttc cggaatacgc gagttacggg tgtgttcata ctgcacccag
     2401 ttgctggtgg tcactccgtt atcccagccg ccgttccagg tcagcgagta gttctggcga
     2461 tacagacggt tggtttcatc gccatatttc gagcgggtat aggaatcgga gttggtgttc
     2521 tgggtgtcgc ccgcatacag gttgccctgg cggctgtaac ctgcttccag ttccagcgat
     2581 tgcaatggcg cgaaatccca gcgcaccacg ccattaatat ctttgttgat caccccttcg
     2641 cgcccggctg gtaacgtcgt ggcatacgtt ccggcacgcg cggactgatg gccctggttg
     2701 atatcccacg cgtcagcctg ggttttgtcg aggttgccat acaagcggaa gctgaattcg
     2761 tcgcccagcg gaccggtcag gctgaagttg gtgcgtttag tggcaccttc ctctttatgt
     2821 tctggtgcat tgaaataagc gtcccaggag ccgtgccact caccgctgcc ttttttggta
     2881 atgatgttaa ccacgccgcc cgccgcgccg ttgccataac gcgcagctgc cggaccacgc
     2941 agaacttcaa tacgttcaat catttcaggt ggcacccagg aggtatcacc acgggtatcg
     3001 cgctcgccac gccagccctg acgcaccgag ttacggctgc ttaccggctt gccgtcaatc
     3061 aaaatcagcg tgttttccgg acccataccg cgaatatcaa tctgtcggtt attcccacgc
     3121 tgaccactgg tggagttacc ggtcaggtta acgcctggca tggtacggat gatctccgac
     3181 acatcgcggg caacggggtt tttgcggatt tcatctgcgg tgatggtcga aacgccaggc
     3241 gcctgtaagt tctgctcggc ggcggtaacg acaatggtat cgtcatgtga aacaggagta
     3301 tcggtcggct cttgtgcctg cgctacccca taaatcccca gattgaccaa caaggccagg
     3361 gaatgaatct tcttgttcat tgttttattc ctgcattttt gccacgaatt gcaactgtcg
     3421 ggcatggtcg tcctcaacac gacgcatccc gctaccgcga aaacctttga tcctgaaaga
     3481 cacgcagtgc agttggttaa taaatgtccg cgcttcccac ggcgcgccat tacgctattg
     3541 caaatgcaaa tggttatcaa taatattatc aatatatttc tgcaatcaat gaaaaattgc
     3601 acagtaaaca tggggttatg gtgtgacggc gttaaaagta ggaagtgaga gctggtggca
     3661 gtcgaaacat ggcccggaat ggcagcgtct gaatgacgaa atgtttgagg tcactttctg
     3721 gtggcgtgat ccccaaggtt ctgaagaata ctcgacgata aagcgcgtat ggatctacat
     3781 cactggtgtg accgatcatc atcagaacag ccagccccag tcgatgcagc gaattgcagg
     3841 tactgatgtc tggcagtgga cgacacaact caatgccaac tggcgcggca gctactgctt
     3901 tattcccacc gaacgcgatg acattttttc tgcaccatcc cccgatcgcc tcgaattacg
     3961 tgaaggctgg cgaaaactat taccccaggc aatagccgat ccgctgaatc cacaaagctg
     4021 gaaaggcggg cgagggcacg ctgtttctgc actcgaaatg ccgcaagcgc ctctgcaacc
     4081 gggatgggat tgtccgcaag tgccagaaat agcagccaaa gaaattatct ggaaaagtga
     4141 acggttgaaa aattcacgtc gtgtatggat ttttaccacc ggcgatgtaa cagcagaaga
     4201 acgtccgctg gcagttttgc tcgatggcga attttgggcg caaagtatgc ccgtctggcc
     4261 tgtgctgact tcgctgaccc atcgtcagca acttcctccc gccgtgtatg tgttgatcga
     4321 cgctatcgac accacgcacc gcgcccacga actgccgtgt aatgcggatt tctggctggc
     4381 agtacagcaa gagttattac ccctggtgaa agctattgcc ccttttagcg atcgcgccga
     4441 tcgcaccgtg gtcgccgggc agagttttgg tgggctgtcc gcgctgtatg ccggactgca
     4501 ctggcctgaa cgctttggct gtgtattaag ccagtcagga tcgtactggt ggccgcatcg
     4561 ggacgggcag caagagggcg tgttacttga aaagctaaaa gctggtgaag ttagcgccga
     4621 aggtctgcgc attgtgctgg aagcgggtat tcgcgagccg atgatcatgc gggccaatca
     4681 ggcgctgtat gcgcaattgc accctataaa agaatccatt ttctggcgtc aggttgacgg
     4741 cggacatgat gcgctttgtt ggcgcggtgg cttgatgcag gggttaatcg acctctggca
     4801 accacttttc catgacagga gttgagtatg gcattcagta atcccttcga tgatccgcag
     4861 ggagcgtttt acattttgcg caatgcgcag gggcaattca gtctgtggcc gcaacaatgc
     4921 ctcttatcgg caggctggga cattgtgtgt cagccgcagt cacaggcgtc ctgccagcag
     4981 tggctggaag tccactggcg tactctgaca ccggccaatt tcacccagtt gcaggaggca
     5041 caatgagcca gcatttacct ttggtcgccg cacagcccgg catctggatg gcagaaaaac
     5101 tgtcggattt accctccgcc tggagcgtgg cgcattacgt tgagttaact ggagatgttg
     5161 atgcgccatt actggcccgc gcggtggttg ccggactagc gcaagcagat acgttgcgga
     5221 tgcgttttac ggaagataac ggcgaagtct ggcagtgggt cgatgatgcg ctgatattcg
     5281 aacagccaga aattatcgac ctgcgaacca atattgatcc gcacggtact gcgcaggcat
     5341 taatgctggc ggatttgcaa caagatttgc gcgtcgatag cggtaaacca ctggtctttc
     5401 atcagctgat acatgttgcg gataaccgct ggtactggta tcagcgttat caccatttgc
     5461 tggtcgatgg cttcagtttc ccggccatta ctcgccagat cgccaatatt tactgcacat
     5521 ggctgcgtgg cgaaccaacg cctgcttcgc cgtttacgcc tttcgctgat gtagtggaag
     5581 agtaccagca ataccgcgaa agcgaagcct ggcagcgtga tgcggcattc tgggcagaac
     5641 agcgtcgtca actgccgccg cccgcgtcac tttctccggc acctttaccg gggcgcagcg
     5701 cttcggcaga tattctgcgc ctgaaactgg aatttaccga cggggaattc cgccagctgg
     5761 ctacgcaact ttcaggtgtg cagcgtaccg atttagccct tgcgctggca gccttgtggc
     5821 tggggcgatt gtgcaatcgc atggactatg ccgccggatt tatctttatg cgtcgactgg
     5881 gctcggcggc gctgacggct agcggacccg tgctcaacgt tttaccgttg ggtattcaca
     5941 ttgcggcgca agaaacgctg ccggaactgg caacccgact ggcggctcaa ctgaaaaaaa
     6001 tgcgtcgcca tcaacgttac gatgccgaac aaattgtccg tgacagcggg cgagcggcag
     6061 gtgatgaacc gctgtttggt ccggtactca atattaaggt atttgattac caactggata
     6121 ttcctggtgt tcaggcgcaa acccataccc tggcaaccgg tccggttaat gaccttgaac
     6181 tggccctgtt cccggatgaa cacggtgatt tgagtattga gatcctcgcc aataaacagc
     6241 gttacgatga gccaacgtta atccagcatg ctgaacgcct gaaaatgctg attgcccagt
     6301 tcgccgcgga tccggcgctg ttgtgcggcg atgtcgatat tatgctgcca ggtgagtatg
     6361 cgcagctggc gcagatcaac gccactcagg ttgagattcc agaaaccacg ctgagcgcgc
     6421 tggtggcaga acaagcggca aaaacaccgg atgctccggc gctggcagat gcgcgttacc
     6481 tgttcagcta tcgggaaatg cgcgagcagg tggtggcgct ggcgaatctg ctgcgtgagc
     6541 gcggcgttaa accaggggac agcgtggcgg tggcactacc gcgctcggtc tttttgaccc
     6601 tggcactcca tgcaatagtt gaagctggcg cggcctggct accgctggat accggctatc
     6661 cggacgatcg cctgaaaatg atgctggaag atgcgcgtcc gtcgctgtta attaccaccg
     6721 acgatcaact gccgcgcttt agcgatgttc ccaatttaac aagcctttgc tataacgccc
     6781 cgcttacccc ccagggcagt gcgccgctgc aactttcaca accgcaccac acggcttata
     6841 tcatctttac ctctggctcc accggcaggc cgaaaggggt aatggtcggg cagacggcta
     6901 tcgttaaccg cctgttatgg atgcaaaatc actacccgct tacaggcgaa gatgtcgttg
     6961 cccaaaaaac gccgtgcagt tttgatgtct cggtgtggga gtttttctgg ccgtttatcg
     7021 caggggcaaa actggtgatg gctgaaccgg aagcgcaccg tgacccgctc gctatgcagc
     7081 aattctttgc cgaatatggc gtaacgacca cgcactttgt gccgtcgatg ctggcggcat
     7141 ttgttgcctc gctgactccg caaaccgctc gccagagttg cgcgacgttg aaacaggttt
     7201 tctgtagtgg tgaagcctta ccggctgatt tatgccgtga atggcaacag ttaaccggtg
     7261 cgccgttaca taatctgtat ggcccgacgg aagcggcggt agatgtcagc tggtatccgg
     7321 cttttggcga ggaactggca caggtgcgcg gcagcagtgt gccgattggt tatccggtgt
     7381 ggaacatggg gctgcgtatt cttgatgcga tgatgcatcc ggtgccgccg ggtgtggcgg
     7441 gtgatctcta tcttaccggc attcaactgg cgcagggcta tctcggacgc cccgatctga
     7501 ccgccagccg ctttattgcc gatccttttg ctcctggcga acggatgtac cgtaccggag
     7561 acgttgcccg ctggctggat aacggcgcgg tggagtacct cgggcgcagt gacgatcagc
     7621 taaaaattcg cgggcagcgt atcgaactgg gcgaaatcga tcgcgtgatg caggcgctgc
     7681 cggatgtcga acaagccgtt acccacgcct gtgtgattaa ccaggcggca gccactggtg
     7741 gtgatgcgcg tcagttggtg ggctatctgg tgtcgcaatc gggcctgccg ttggatacca
     7801 gcgcattgca ggcgcagctt cgtgaaacat tgccaccaca tatggtaccg gtggttctgc
     7861 tgcaacttcc acagttacct cttagcgcca acggcaagct ggatcgcaaa gccttaccgt
     7921 tgcctgaact gaagacacaa gcgtcgggac gtgcaccgaa agcgggcagt gaaacgatta
     7981 tcgccgcggc attcgcgtcg ttgctgggtt gtgacgtgca ggatgccgat gctgatttct
     8041 ttgcgctggg cggtcattcg ctactggcaa tgaaactggc agcgcagtta agtcgacagt
     8101 ttgctcgtca ggtgacgccg gggcaggtga tggtcgcgtc aaccgtcgcc aaactggcaa
     8161 cgattattga tggtgaagag gacagctccc ggcgcatggg attcgaaacc attctgccgt
     8221 tgcgtgaagg taatggcccg acgctgtttt gtttccatcc ggcgtccggt tttgcctggc
     8281 agtttagcgt actctcgcgt tatctcgatc cacaatggtc gattattggc attcagtcgc
     8341 cgcgccctca tggacccatg cagacagcga caaacctgga tgaagtctgc gaagcgcatc
     8401 tggcaacgtt acttgagcaa caaccgcacg gcccttatta cctgctgggg tattcccttg
     8461 gcggtacgct ggcgcagggt attgcggcgc gactgcgtgc ccgtggcgaa caggtggcat
     8521 ttcttggctt gctggatacc tggccgccag aaacgcaaaa ctggcaggaa aaagaagcta
     8581 atggtctgga cccggaagtg ctggcggaga ttaaccgcga gcgcgaggcc ttcctggcgg
     8641 cacagcaggg aagtacttca acggagttgt ttaccaccat tgaaggcaac tacgctgatg
     8701 ctgtgcgcct gctgacgact gctcatagcg taccgtttga cggtaaagcg acgttgtttg
     8761 ttgctgaacg cacgcttcag gaaggtatga gtcctgaacg cgcctggtcg ccgtggattg
     8821 cggagctgga tatctatcgt caggattgtg cgcatgtgga tattatctct ccaggggcgt
     8881 ttgtaaaaat tgggccgatt attcgcgcaa cgctaaacag gtaaattaat attatctata
     8941 aacccataat tacagaaaat aattatgggt tttttatttg tttgatttat aggtttgatg
     9001 aatatttctc ttaaatagag tgaatattgc attcagtgga aggaatttac aaacttcagc
     9061 ttcggataat tctcaccaac tggtaaagtg agcgccgtgg agattttccc ctattggact
     9121 cattttcagg ttatacccca tgtcatcatt gaatattaaa cagggaagtg acgctcattt
     9181 ttctgagtat cctctggcgt cgcccagtaa taatgaaatt gatttactta atctaatcga
     9241 agttttatgg cgggccaaaa aaacggtcat ggcggtcgtt tttgcatttg cctgcgcagg
     9301 tttactgatc tctttcatcc tgccgcaaaa atggaccagc tcagccgtta ttactcccgc
     9361 cgaagccatt cagtggcagg acctggagaa aacctttacc aaactccgcg ttcttgatct
     9421 cgatgttaat atcgaccgtg gcggtgcatt taacctgttt atcaagaagt ttcagtctgt
     9481 tagcttgctg gaagagtacc tgcgttcatc accttatgtg atggaccaat taaaagaggc
     9541 gaaaatcgat gaactggatt tgcatcgcgc tattgttgca ttgagcgaaa aaatgaaagc
     9601 ggttgatgac aatgccagta agaaaaaaga tgaaccgtca ctgtatacct cctggacgct
     9661 aagttttacc gcgccaacca gtaaagaggc gcagaccgtt ttgagcgggt atatcgatta
     9721 tatctctgcg ttggtggtga aagagtcgat agaaaacgtc cgtaataaac tggagatcaa
     9781 aacccagttt gaaaaagaaa aactggctca ggatcgcatt aaaacgaaaa atcaacttga
     9841 tgcaaacatt cagcgcctca attattcact cgacattgcc aacgcggcag gaattaaaaa
     9901 gcccgtatac agtaatggtc aggccgttaa agatgacccc gatttctcta tttctctcgg
     9961 cgcagacggt attgaacgca aactggaaat tgaaaaagcg gttactgacg ttgcggaact
    10021 gaacggtgaa ttacgtaatc ggcagtatct tgtcgagcaa ttaacaaaag caaatgtcaa
    10081 cgatgtgaat tttacgccgt ttaaatatca gttaagcccg tcattgccag tgaaaaaaga
    10141 cggtccgggt aaggcgatta ttgtgatcct ttccgcgctg atcggcggga tggtggcttg
    10201 tggtagcgtg ttattgcgct atgcgatggc atccagaaaa caggatgcca tgatggcaga
    10261 ccacttagtt taactatttg cggttgaagg tgccgttcgt ccgagcggca ccacaagcgg
    10321 cgttccggct accggatcgt caatgatcat gcatcgcaga ccataaatgc gctcaatcag
    10381 ttcagcagtg acaatctcct tcggcgctcc ctgagcaaca attttccctt cccgcaatgc
    10441 aatcaaatgg ctggcgtaac gacaggcctg attaagatcg tgcagcaccg ccgccagggt
    10501 atagcctttc tcgcggttca gttcgcttaa cagttccagt agatcaatct gatgactgat
    10561 atccagccag gtcgtgggtt cgtcgagcag cataattgcc gtttcctggg ctagcaccat
    10621 cgcgatccac gctcgctggc gttgtccgcc agaaagggta tccacgcttt gatctgccag
    10681 atgagttatt ctcgtggcct gcatcgcttt cgttaccgct tcttcatcct ctttgcgcca
    10741 gcgggtaaac agcggttgat gcggataacg tccacgcgcc accagctcct gcacggtgat
    10801 atcgcccggc gtggtagcat tttgcgccaa cagaccaatc cggcgtgcaa cttctttact
    10861 ggcgtaatgt tgaatgtgct cgccatccag ccagacatgc ccgtgagcag gcgtcatcag
    10921 gcggcttaag gtacgcagta acgtggattt accacagcca tttggtccga taattgccgt
    10981 gaagtgacca tcaggaattt ctacagtcag attttccgca acggtatatt tgccatatcc
    11041 cagggttaac tgttcgccgc gcaaacgggc tactgattcg gtcatttttt gcgagactcc
    11101 tgaattaaca agacgataag gtaaataccg ccgaggctga cggtaacgac gccaaccgga
    11161 agttgatacg gcataaacag ttgttgggcg cagagatcgg ccgccagcag taacagcgct
    11221 ccgcacagcg ccgcctgggt cagcccccag cgagcggtgc cgctaatgcg tcgggcaatg
    11281 tgcggcgcga ctaaagcaat aaaggaaatc ggcccggcaa gcgccgttgc cgcagcggta
    11341 agcaccactg caaccagcat cattaacaga cgcgaacgct cgacgctgac gcccagcgcg
    11401 caggcggtat catcgcccat ctccagcaag cgcattcgcc gcaccagcaa ggcggcggca
    11461 atgagcatca atatgatgat gggtgcggaa ggcgaagttt ttgcccacgt caggccattg
    11521 agcgatccgg cattccacaa acccgctgtt agcgccgttt ctaaagacgc tttcagcaac
    11581 agccaagtat taaaggccac cagcatggcg cgaacgccga tgccgataat aatcaaccga
    11641 aaggtgtcga taccgttacg ccaggcgagc agccagacca acagagaagt gacaatgccg
    11701 cccaccattg ctgccagcgc gatagccgtc aggtcctgac caaacagcac catcgccacc
    11761 agcacgccgc tccacgcccc ggtgttaaag cccattacgt cagggctgcc gagcgggtta
    11821 cgcatcagcg actgaaaaat cgcgccgctg acgcccagcg ccgcgccaat caacagcgcc
    11881 atcagcacgc gtggtaaacg ccattcggtg accaccatcg tcatactgcg cggcgcatcg
    11941 cccatcaacg cggcgaatac ctgcgagatt tccagcgtga cggcaccgct gcgtaatccc
    12001 cagatacctg caaccacaca ggcggaaatc agcagcaaac aggtgatgag taatcggcga
    12061 gagacgtaaa tcatgcacca cctcgcgttt tacgtcgcac gaggaagatc agcaccggtg
    12121 caccaataaa cgcactgacc acagaaacgc gcagttcgcc gggcacaatc acccgcccga
    12181 tgatatcggc aaacagcagc agggcagggg tagcaagtag cgtgacgggc agcgaccagc
    12241 gatgatcggc acctaccagc cagcgcgcca tgtgcggcat catcaggccg ataaaggcaa
    12301 tcgggccaac taccgccgtc gcactaccac aaagcacggt aatcgccagc agaccaatca
    12361 actgtgtgcg cgccacgcga ctgcccagcg ccgtcgcggt gtcactgccg aggctcaaac
    12421 tgttcagcgc gcgactcagt aatagcgcag ttgctccggc gatcagaacc gggatcagca
    12481 ccacttttaa ggtatgtaga ttgcgaatat ccagcgaacc ggcttgccag aaacgcagtt
    12541 gatcgtagac gtcaggatta agcagggcga tgccgctggt cagcccttcc agcaccgccg
    12601 ccagcgccac gcccgccagg gttaaacgca ccggacttaa ctgcccgccg ccctgactgc
    12661 cgataaaggc aacaatcaat gaggccacca gcgccccggc gaaggccatc gccagttgtt
    12721 cctgcgcgga tgagtaacca aacagcgccg cacccagcac aatggcaaag ctggctccgg
    12781 cgttcacgcc aagcaagccg gggtcggcaa gtggatttcg ggtgagggtt tgcattaacg
    12841 ccccagcaag cccaagcgca ccgcctgcaa gtaaaccggc aagggtgcgc ggcagtcgcg
    12901 cgtcgagcac gatggtgcag tcggcgctct ggcaggtgcc tgagaaggcc tccagcacta
    12961 ctgacgcggg gagtgatttt gccccaatga gcaggcttaa tgccgtcgcg ataatcagta
    13021 ataacagcaa tccgggcacg gcaatggcgc gtgtcacggc aacagaacca gacatacgaa
    13081 cttccatgat aatgaaatta attatcgtta tcgatcttat ttggatatgt tagcatgtgc
    13141 agcctaagaa taggtattta aaatatttga tggcaaggca ttgtaatgaa taaacaatcc
    13201 tggctgctta acctcagcct gttgaaaacg cacccggcgt ttcgcgcagt attcctcgct
    13261 cgttttatct caattgtgtc tctgggtttg ctcggcgtcg cggtgccggt gcagatccag
    13321 atgatgacgc attctacctg gcaggtgggg ctttcggtga cgctgaccgg cggcgcgatg
    13381 tttgttggcc tgatggttgg cggtgtgctg gcggatcgct atgagcgcaa aaaagtgatt
    13441 ttgctggcgc gcggcacctg tggcattggc ttcattggac tgtgcctgaa cgcgctgctg
    13501 ccggagccgt cattgctggc aatctattta cttggtttat gggatggttt tttcgcatca
    13561 cttggtgtta cggcgctact ggcggcaaca cctgcactgg tagggcgtga aaacttaatg
    13621 caggccgggg cgatcaccat gttgaccgtg cgtctggggt cggtgatttc gcccatgatt
    13681 ggcggtttat tgctggcgac cggcggcgta gcctggaact acgggctggc ggcggcgggc
    13741 acgtttatta ccttgctacc gttgttaagc cttccggcgt tgccaccgcc accgcagccg
    13801 cgcgagcatc cgttgaaatc attactggca ggatttcgtt ttctgctcgc cagcccgctg
    13861 gtaggaggga ttgcgttgct gggtggttta ttgacgatgg cgagcgcggt gcgggtactg
    13921 tatccggcgc tggctgacaa ctggcagatg tcggcggcac agattggttt tctctatgcg
    13981 gcgatcccgc tcggtgcggc tattggtgcg ttaaccagcg ggaagctggc acatagtgcg
    14041 cgaccagggt tattgatgct gctctccacg ctgggatcgt tcctcgccat tggtctgttt
    14101 ggcctgatgc cgatgtggat tttaggcgtg gtttgtctgg cgctgttcgg ctggctgagc
    14161 gcggttagtt cgttgctgca atatacaatg ctgcaaacgc aaaccccgga agcgatgtta
    14221 gggcggatta acggtttgtg gacggcgcag aacgtgacgg gcgatgccat aggcgcggcg
    14281 ctgttgggtg gtttgggcgc gatgatgaca ccggttgctt ccgcaagcgc gagcggtttt
    14341 ggtttgttga ttatcggcgt gttgttattg ctggtgctgg tggagttgcg acgttttcgc
    14401 cagacgccgc cgcaggtgac agcgtctgac agttagctaa aggtgcattt tcccctcacc
    14461 ctaaccctct ccccaaaggg gcgaggggat tattagtgct ctaattgtat gttttatcaa
    14521 tcttttttta acagtgaatt aagtctgtcc agcacttgca tggcgctgta gtaatccagg
    14581 cggaacgtct cggttcccag cgtataaacc tgcttgtttt gtactgcagg caggtgcgcg
    14641 agcagtggat tggcataaat cgcatcggcg tctttctgat caccggcgaa caggaatagc
    14701 gattcaccgt ttaacccagc agccagattt tccccaccaa gctgaatgat gtcatggcgt
    14761 ttgccctggc tttggctggc gtttaaccct gcgggcaact tcgccagcgt aaagccgagt
    14821 tgttccagca tctgcccttg tgctgattcc ggcgtccaga gattggcact gtgtgcagcg
    14881 gcggtataga caatggcagt gaccggctgc ggcggtaatt tgatttgctc tttcgccgct
    14941 gccagttgct tatcaaactg tgcaatccgc tctgccgctt gtttctcatg cccggtaatt
    15001 tcgccaagct gcgttaacag cgactgccag cttttgtcgt cgtaattgat gattaatgtc
    15061 ggggcgatgg tggaaagctg atcatacagc gccagcgccg aatccccgcc ggttgcgcta
    15121 attaaaatca gatccggcat ttgcgcggca acggcttcgg cgctcggttc gccgatatag
    15181 agccgttgca gtttgcgttc tttcgccacc ttgctccact ggcgtaaaaa gccctggtca
    15241 tctgcgacgc ggttattcgg cgtggtcgcg ccgctggcga tcaccggagc atcaatcgcc
    15301 agcagtgatc cggtcagggt gacgctggtg gaaacaatac gctgcggctg gctttccagg
    15361 gtatgagtgc cccggctgtc agtaatctga cgcggccagt cggcggcctg aactacggct
    15421 attcctgaaa gcaaaagtcc tgttaataga agggcgttgc ggtagagcgg ggcgagtctc
    15481 acaagtcagc ttcctgttat taataaggtt aagggcgtaa tgacaaattc gacaaagcgc
    15541 acaatccgtc ccctcgcccc tttggggaga gggttagggt gaggggaaca gccagcactg
    15601 gtgcgaacat taaccctcac cccagccctc accctggaag ggagaggggg caaaacggcg
    15661 caggacatca cattgcgctt atgcgaattc atcaataatg cttctcattt tcattgtaac
    15721 cacaaccaga tgcaaccccg agttgcagat tgcgttacct caagagttga catagtgcgc
    15781 gtttgctttt aggttagcga ccgaaaatat aaatgataat cattattaaa gcctttatca
    15841 ttttgtggag gatgatatgg atacgtcact ggctgaggaa gtacagcaga ccatggcaac
    15901 acttgcgccc aatcgctttt tctttatgtc gccgtaccgc agttttacga cgtcaggatg
    15961 tttcgcccgc ttcgatgaac cggctgtgaa cggggattcg cccgacagtc ccttccagca
    16021 aaaactcgcc gcgctgtttg ccgatgccaa agcgcagggc atcaaaaatc cggtgatggt
    16081 cggggcgatt cccttcgatc cacgtcagcc ttcgtcgctg tatattcccg aatcctggca
    16141 gtcgttctcc cgtcaggaaa aacagacctc agcccactgt tttacccgca gccagtcgct
    16201 gaacgtggtg gaacgccagg caattcctga acaaaccacg tttgaacaga tggttgcccg
    16261 cgccgccgca cttaccgcca cgccgcaggt cgacaaagtg gtgttgtcac gattgattga
    16321 tatcaccact gacgccgcca ttgatagtgg cgtattgctg gaacggttga ttgcgcaaaa
    16381 cccggttagt tacaacttcc atgtcccgct ggctgatggt ggcgtcctgc tgggggccag
    16441 cccggaactg ctgctacgta aagacggcga gcgttttagc tccattccgt tagccggttc
    16501 cgcgcgtcgt cagccggatg aagtgctcga tcgcgaagcg ggtaatcgtc tgctggcgtc
    16561 agaaaaagat cgccatgaac atgaactggt gactcaggcg atgaaagagg tactgcgcga
    16621 acgcagtagt gagttacacg ttccctcctc tccacaattg attaccacgc cgacgctgtg
    16681 gcatctcgca actccctttg aaggtaaagc gaattcgcaa gaaaacgcac tgactctggc
    16741 ctgtctgctg catccgaccc ccgcgctgag cggtttcccg catcaggccg cgacccaggt
    16801 tattgctgaa ctggagccgt ttgaccgcga actgtttggc ggcattgtgg gttggtgtga
    16861 cagcgaaggt aacggcgaat gggtggtgac catccgctgc gcgaagctgc gggaaaatca
    16921 ggtgcgtctg tttgccggag cggggattgt gcctgcgtcg tcaccgttgg gtgagtggcg
    16981 cgaaacaggc gtcaaacttt ctaccatgtt gaacgttttt ggattgcatt aaggagcgag
    17041 gatgagcatt ccattcaccc gctggccgga agagtttgcc cgtcgctatc gggaaaaagg
    17101 ctactggcag gatttgccgc tgaccgacat tctgactcgc cacgctgcga gtgacagcat
    17161 cgcggttatc gacggcgagc gacagttgag ttaccgggag ctgaatcagg cggcggataa
    17221 cctcgcgtgt agtttacgcc gtcagggcat taaacccggt gaaaccgcgc tggtacaact
    17281 gggtaacgtc gctgaacttt acattacctt tttcgcactg ctgaaactgg gcgttgcgcc
    17341 ggtgctggcg ttgttcagcc atcagcgtag tgaactgaac gcctatgcca gccagattga
    17401 accggcgtta ctgattgccg atcgccaaca tgcgctgttt agcggggatg atttcctcaa
    17461 tactttcgtc acagaacatt cctccattcg cgtggtgcaa ctgctcaacg acagcggtga
    17521 gcataacttg caggatgcga ttaaccatcc ggcagacggt tttactgcta cgccatcacc
    17581 tgctgatgaa gtggcctatt tccagctttc cggcggcacc accggtacac cgaagctgat
    17641 cccgcgcact cataacgact actactacag cgtgcgtcgt agcgtcgaga tttgtcagtt
    17701 cacacaacag acacgctacc tgtgcgcgat cccggcggct cataactacg ctatgagttc
    17761 gccaggatcg ctgggcgtct ttcttgccgg aggaacggtt gttctggcgg ccgatcccag
    17821 cgccacgctt tgcttcccgt tgattgcaaa acatcaggtt aacgttaccg cgctggtgcc
    17881 gccggcagtt agcctgtggt tgcaggcgct ggctgaaggc gaaagccggg cgcagcttgc
    17941 ctcgctgaaa ctgttacagg tcggcggcgc acgtctttct gccacccttg cggcgcgtat
    18001 ttccgctgag attggctgcc agttgcagca ggtgtttggc atggcggaag ggctggtgaa
    18061 ctacacccga cttgatgata gcgcggagaa aattatccat acccagggtt acccaatgtg
    18121 tccggacgat gaagtatggg ttgctgatgc tgaaggaaat ccactgccgc aaggggaagt
    18181 tggacgcctg atgacgcgcg ggccgtacac cttccgcggt tattacaaaa gtccgcagca
    18241 caatgccagt gcctttgatg ccaacggttt ttactgttcc ggcgatctga tctctattga
    18301 tccagagggt tacatcaccg tgcaggggcg cgagaaagat cagatcaacc gtggcggcga
    18361 gaagatcgct gccgaagaga tcgaaaacct gctgctgcgc catccggcgg tgatctatgc
    18421 cgcactggtg agcatggaag atgagctgat gggcgaaaaa agctgtgctt atctggtggt
    18481 aaaagagccg ctgcgcgcgg tgcaggtgcg tcgtttcctg cgtgaacagg gtattgccga
    18541 atttaaatta ccggatcgcg tggagtgtgt ggattcactt ccgctgacgg cggtcgggaa
    18601 agtcgataaa aaacaattac gtcagtggct ggcgtcacgc gcatcagcct gaaggagaga
    18661 acacgatggc tattccaaaa ttacaggctt acgcactgcc ggagtctcac gatattccgc
    18721 agaataaagt tgactgggcc tttgaaccgc aacgtgccgc gttgttaatc catgatatgc
    18781 aggactattt tgtcagcttc tggggcgaga actgcccgat gatggagcag gtgatcgcga
    18841 atattgctgc gctgcgcgac tactgcaaac agcacaatat cccggtttat tacaccgccc
    18901 agccgaaaga gcagagcgat gaagatcggg cgctgttgaa tgatatgtgg gggccgggcc
    18961 tgacccgttc gccggaacag caaaaggtgg tggatcgcct gacgccagat gccgacgaca
    19021 cggtgctggt gaagtggcgc tacagcgcgt ttcatcgttc tccgctggag caaatgctca
    19081 aagagagtgg acgtaaccag ctgattatta ccggggtata tgcccacatt ggctgtatga
    19141 ccaccgcaac cgacgcattt atgcgcgata ttaaaccgtt tatggtggcg gatgcgctgg
    19201 ccgatttcag ccgtgacgag catttgatgt cgctgaaata tgtggccggg cggtctggcc
    19261 gggtggtgat gactgaagga ttactgccag cacctgtccc cgccaggaaa gcggcgctgc
    19321 gtgaggttat cctgccgttg ctggacgagt ccgatgaacc gttcgatgac gacaacctga
    19381 tcgactacgg tctggattcg gtgcgcatga tggcgctggc ggcgcgctgg cgcaaagtgc
    19441 atggcgatat cgactttgtc atgctggcga aaaacccgac catcgacgcc tggtggaagc
    19501 tactctcccg cgaggtgaaa taatggattt cagcggtaaa aatgtctggg taaccggcgc
    19561 aggtaaaggt atcggctacg ccacggcgct ggcgtttgtt gaagcgggag cgaaagttac
    19621 cggttttgat caagcgttcg ctcaggagca atatcccttt gcgaccgaag tgatggatgt
    19681 tgccgatgct gcgcaggtcg cgcaggtatg tcagcgactg ttagctgaaa cggagcgact
    19741 ggacgtgctg gtcaatgcgg cgggaatttt gcgcatgggc gcgaccgatc agctcagtaa
    19801 agaggactgg cagcagactt ttgcggttaa cgtcggcggt gcgtttaacc tgtttcagca
    19861 aaccatgaac cagtttcgcc gtcagcgggg cggggcgatt gtcactgtgg cgtccgacgc
    19921 cgcacacacg ccgcgtattg gtatgagtgc ttatggcgca tcgaaagcgg cgctgaaaag
    19981 cctggcgttg agcgtcgggc tggaactggc ggttagcggc gtgcgctgta atgtggtttc
    20041 gcctggctcc accgacaccg atatgcaacg cacgctgtgg gtgagcgatg acgccgaaga
    20101 acagcgtatt cgcggctttg gcgagcagtt taaactcggc attccgctgg ggaaaatcgc
    20161 ccgtccacaa gagatcgcca acacgatttt gttcctcgcc tctgacctcg ccagccatat
    20221 caccctacag gatattgtgg tcgatggcgg ctcaacgctg ggggcataag catgatctgg
    20281 aaacgccatt taacgctcga cgaactgaac gccaccagcg ataacacaat ggtggcgcat
    20341 ctgggaattg tgtatacccg tctgggagat gatgtgctgg aagccgaaat gccggttgat
    20401 acccgtactc atcagccgtt tggcctgctg catggcggcg cgtcggcggc gctggctgaa
    20461 acgctgggat cgatggccgg atttatgatg acccgtgacg ggcagtgtgt ggtgggtacg
    20521 gaacttaacg ccacccatca tcgcccgatg tctgaaggca aggtacgcgg cgtctgccag
    20581 ccgctgcatc ttgggcggca aaatcagagc tgggaaatca tcgttttcga tgaacagggg
    20641 cggcgttgct gcacttgtcg gctgggtacg gcagttttgg gatgagcccg acagaattaa
    20701 atgagattgc aggaaaactc ggttaacgga gtgatcgagt taacattgtt aagttaaata
    20761 ttggtttcaa ctccgattta catggttacc gtgttgttaa atcgtacaaa gatgttatag
    20821 aaacaaaatg taacatctct atggacacgc acacggataa caactatgaa caaatcaggg
    20881 aaatacctcg tctggacagt gctctctgta atgggagcat ttgctctggg atacattgct
    20941 ttaaatcgtg gggaacagat caacgcgctg tggattgtgg tggcgtcggt ctgtatctat
    21001 ctgatcgctt accgttttta tgggctgtat atcgccaaaa atgtgctggc ggttgacccg
    21061 acgcgtatga cgccagcggt gcgccataac gacgggctgg actatgtgcc gacggacaag
    21121 aaagtgctgt tcggtcacca ttttgcggcc attgccggag cagggccgct ggtggggccg
    21181 gtgctggcag cgcaaatggg ctacctgccg gggatgatct ggctgctcgc cggggtggtt
    21241 ctcgccggtg cggtgcagga tttcatggtg ctgtttgttt ctacgcgccg tgacggtcgc
    21301 tcgctgggtg agctggtcaa agaagagatg gggccaactg ccggggtgat tgcgctggta
    21361 gcctgcttta tgatcatggt cattatcctt gcagtactgg cgatgatcgt ggtgaaagcc
    21421 ctgactcata gcccgtgggg aacgtatacc gttgcgttca ccattccgct ggcgattttt
    21481 atgggaatct acctgcgcta tctgcgtccg gggcgtattg gcgaagtgtc agtgattggc
    21541 ctggtgttcc ttatttttgc cattatctct ggcggctggg tggcagaaag cccgacctgg
    21601 gcaccgtact ttgactttac cggcgtgcag ctgacctgga tgctggtggg ctacggtttt
    21661 gtggcggcgg tgctgccggt gtggttactg ctggccccgc gtgactacct ctctaccttc
    21721 ctgaaaatcg ggactatcgt tggtctggcg gtaggcattt tgattatgcg tccgacgctg
    21781 accatgcctg cgctgaccaa atttgtcgat ggcactggcc cggtatggac cggtaatctg
    21841 ttcccgttcc tgtttatcac catcgcctgt ggcgcggtgt caggcttcca tgcgctgatc
    21901 tcttccggca ccacgccgaa gatgctggcg aacgaagggc aggcgtgctt tatcggctac
    21961 ggtggaatgt taatggaatc cttcgtggcg attatggcac tggtttccgc ctgtatcatc
    22021 gatccgggcg tgtacttcgc catgaacagc ccgatggcgg tgctggctcc ggcagggacg
    22081 gcggatgtgg tcgcttctgc cgcgcaggtg gtgagtagct ggggctttgc gattactccg
    22141 gatacgctca accagattgc cagcgaagtg ggtgaacagt cgatcatttc ccgtgcgggc
    22201 ggtgcgccga cgctggcggt ggggatggct tacatcctgc acggtgcgct gggtggtatg
    22261 atggatgtgg cgttctggta tcacttcgcc attctgtttg aagctctgtt tattctgacg
    22321 gcggtagatg cgggtacgcg tgctgcgcgc tttatgttgc aggatctgct gggcgtggtg
    22381 tctcctggcc tgaagcggac cgattcactg cctgctaacc tgctggcaac agcgctgtgc
    22441 gtactggcgt ggggctactt cctccatcag ggcgtggtcg atccgctggg cggcattaac
    22501 actctgtggc cgctgtttgg tattgccaac cagatgctgg cagggatggc gctgatgctc
    22561 tgtgccgtgg tgttgttcaa gatgaaacgt caacgttacg cctgggtggc gctggtacca
    22621 acggcctggc tgctgatttg taccctgacc gcaggctggc agaaagcgtt tagcccggat
    22681 gcgaaagtcg gcttcctggc cattgctaat aagttccagg caatgataga cagcggcaat
    22741 attccgtcgc agtatactga gtcacagctg gcgcaactgg tgttcaacaa ccgtctggat
    22801 gccgggttaa ccatcttctt tatggtggtc gtggtggttc tggcgctgtt ctcgattaag
    22861 tcggcactgg cggcattgaa agatccgaag ccaacggcga aagaaacgcc gtatgagcca
    22921 atgccggaaa atgtcgagga gatcgtggcg caggcaaaag gcgcacacta aagtcagagt
    22981 gaggggggat gttggcgaat gttggcatag tgccgagggt tcctcctcac cctaaccctc
    23041 tccccgatgg ggcgagggga ctgaccgagc gcgttgatag catttgtagg ccggataagg
    23101 cgttcacgcc gcatccggca ctctttcagc aacatggtta gcggaggcca agatgtttga
    23161 ttcactggca aaagccggaa aatatttagg tcaggcggcg aagctgatga ttggtatgcc
    23221 tgattacgac aactatgtcg aacatatgcg ggttaaccat cccgatcaaa cgccgatgac
    23281 ctacgaagag tttttccgtg agcggcagga cgcgcgctac ggtggaaaag gcggcgcgcg
    23341 ctgctgctaa atttgcacgt caggctttaa acgattccac tttttcgaac gctgcacgca
    23401 acgtatctgg tgtcagcgtg actggcaggt aatgaatgta ttccaccgga cgcagggtgt
    23461 gggtaatcac tttgtcgatc tccgcatgat tattgatatc catttccagc tccgccagcg
    23521 tggtcggcag atgaaaacgc tgatacgccc cggttaattg cgccagcaca tcatcctgac
    23581 ctagcaaggc gctttgtacc agaatgccat aagcgacttt ggtgccgtgg agaaatttct
    23641 cggtttgcgg caggacagtc agaccgttat gcacggcatg agccgccgcc acacgcgtaa
    23701 aacgatcgcc cagatcaccg accatcccac caccagcaat aatagcatcc accacatcgc
    23761 aaaatgattg cgttaactgt tgattttgct gatcgctcag cgcctgttcg ctactgttta
    23821 acaatacgtc gcgaatggcc tgcgcattat tgatccccag tcgcacggtt agcggcaacg
    23881 tttctggttg cggagccagc accaccgctt cataccattt cgccagcgtg tcaccgatcc
    23941 ccgccagcag atattgttgc ggcgcgttga ggataatctc tggttccacc agcaccataa
    24001 aattggcgtc gtcgaaaatc tcataatgca gcgcctgtcc ggcatcgtta taccagacgg
    24061 agagcggtgt ccaggcggcg caggtagcgg cgatcgtcgg aacggcaaca aatggcagac
    24121 cgagac
//
LOCUS       DAJTZL010000048        23844 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.048, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000048 DAJTZL010000000
VERSION     DAJTZL010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23844
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.048"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(70..1323)
                     /gene="amiC"
                     /locus_tag="OO544_001693"
     CDS             complement(70..1323)
                     /gene="amiC"
                     /locus_tag="OO544_001693"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HCR6767312.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            1555..2886
                     /gene="argA"
                     /locus_tag="OO544_001694"
     CDS             1555..2886
                     /gene="argA"
                     /locus_tag="OO544_001694"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HCR6767313.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IRRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQLDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(2948..4774)
                     /gene="recD"
                     /locus_tag="OO544_001695"
     CDS             complement(2948..4774)
                     /gene="recD"
                     /locus_tag="OO544_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HCR6767314.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEESLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERAGQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHSHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPIVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRG"
     gene            complement(4774..8316)
                     /gene="recB"
                     /locus_tag="OO544_001696"
     CDS             complement(4774..8316)
                     /gene="recB"
                     /locus_tag="OO544_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HCR6767315.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYEHLLE
                     EVDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIDKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDRHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(8309..11197)
                     /gene="ptrA"
                     /locus_tag="OO544_001697"
     CDS             complement(8309..11197)
                     /gene="ptrA"
                     /locus_tag="OO544_001697"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HCR6767316.1"
                     /translation="MPRSTWFKALLLLVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPAMPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISEQTFADWQQKAANIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFSSEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(11373..14741)
                     /gene="recC"
                     /locus_tag="OO544_001698"
     CDS             complement(11373..14741)
                     /gene="recC"
                     /locus_tag="OO544_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HCR6767317.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFSIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHQLGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIRAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLSAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(14754..15077)
                     /locus_tag="OO544_001699"
     CDS             complement(14754..15077)
                     /locus_tag="OO544_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HCR6767318.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVMIVTALSGIQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(15062..15469)
                     /locus_tag="OO544_001700"
     CDS             complement(15062..15469)
                     /locus_tag="OO544_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HCR6767319.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSIESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDARVCLRLLADNEALLVAGYEGVSL
                     WRTGEVIDGNIVFSPRGWSDFCPLKERALCQLP"
     gene            complement(15466..16029)
                     /locus_tag="OO544_001701"
     CDS             complement(15466..16029)
                     /locus_tag="OO544_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HCR6767320.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(16020..16490)
                     /locus_tag="OO544_001702"
     CDS             complement(16020..16490)
                     /locus_tag="OO544_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HCR6767321.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISVIREGTLWCLVSSAAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIRFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(16675..17469)
                     /gene="thyA"
                     /locus_tag="OO544_001703"
     CDS             complement(16675..17469)
                     /gene="thyA"
                     /locus_tag="OO544_001703"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HCR6767322.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(17476..18351)
                     /gene="lgt"
                     /locus_tag="OO544_001704"
     CDS             complement(17476..18351)
                     /gene="lgt"
                     /locus_tag="OO544_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HCR6767323.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(18502..20748)
                     /gene="ptsP"
                     /locus_tag="OO544_001705"
     CDS             complement(18502..20748)
                     /gene="ptsP"
                     /locus_tag="OO544_001705"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR6767324.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDYAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(20761..21291)
                     /gene="rppH"
                     /locus_tag="OO544_001706"
     CDS             complement(20761..21291)
                     /gene="rppH"
                     /locus_tag="OO544_001706"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HCR6767325.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNTSAYRRKRG"
     gene            21976..22665
                     /gene="mutH"
                     /locus_tag="OO544_001707"
     CDS             21976..22665
                     /gene="mutH"
                     /locus_tag="OO544_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HCR6767326.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            22734..23447
                     /locus_tag="OO544_001708"
     CDS             22734..23447
                     /locus_tag="OO544_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCR6767327.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            23585..23803
                     /gene="ygdR"
                     /locus_tag="OO544_001709"
     CDS             23585..23803
                     /gene="ygdR"
                     /locus_tag="OO544_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HCR6767328.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
BASE COUNT         5698 a         6526 c         5960 g         5660 t
ORIGIN      
        1 tgaacaccct aacgggtgtt tttttgtttc tactctccca taaaaaaagc gccattcagc
       61 gcctttttat catccccttc tcgccagcgt tgccccatcg gcaaaatacg ctttaatccc
      121 cgcaagaata gactccgcaa cttcctgctg gaaagtcgcc gttttcagtt tacgctcttc
      181 ctcaacgtta ctgataaacg ccgtttcgac cagaatggag ggaatatctg gtgcctttag
      241 tacggcaaac ccggcctgtt caacttgatt tttatgcagc ttgttgattt taccgagctt
      301 attcagcact gctttaccaa acttaaggct gtcagcaatg gtcagcgact gcaccatatc
      361 gaacatggtg tggtcgacat agcggtcacc gcttttgctc acgccaccaa tcaagtccga
      421 ggcgttctgg gtttgtgcca gatattttgc cgcagtactg gttgcacctt tggttgagag
      481 cgcaaacacc gaggaaccgc tcggctggcg actggtaaag gcgtcggcat ggatagagac
      541 aaacaggtca gcacgctgtt tctgggcttt tgctacgcgc acttgcaacg gaatgaagat
      601 gtcttcattg cgcgtcatgt acaccttcat attgccctct ttctcgatca gagagcgcaa
      661 acggcgagct atttgcaata ccacatcttt ttcgcgcgtt ttgtatttcc ccaccgcacc
      721 ggagtcttcg ccaccgtggc cagggtcaag cataatgaca atcggacgat cgcgccctgc
      781 tttacctggt tgtggaccac tttgcgctgg cggcacctgc ttttcgaggt cgcctttgtt
      841 gtaatcctcc agcagcgcca gcagcgggtc ctgcatatcc tgtgcattgg ccggatagag
      901 gtccatcacc agacgctctt taaacccggc gaccggcgca agggcaaaca gctgcggttt
      961 tacgttttgc tttaattcaa aaaccatccg tacggtttgc gggtcaaatt gcccgacgcg
     1021 cgccgacttg atgaacgggt cgtctgcacg aatttgtgcc gccatcccct tgagtaccga
     1081 gttcaggttt acatcttcga tatccaccac cacgcgttca gggttactca acgcgaactg
     1141 cttatatttc agctgacgat ttgattctac cgtcacgcgg gtgtagctgg acgcaggcca
     1201 gacgcgcacc gccacgacct ggctaaccgc agccaggctg acctgactta cgctcaatag
     1261 ccacatagcg cccgcgcctt gcagtaaacg acgacggctg attgcagtgt tggatcctga
     1321 catgcctctc ccgagcaaaa gaaatctaag ctgtgtaaca agtaaacgac taatttgacc
     1381 ggaaacttta gcgaaagacg cataatctgt catctaataa acggtaaaca ttctttttat
     1441 attcacggca ttactgataa aaaagtcgct ctcgcataaa atttacactt gcaccctgcg
     1501 aaaaaacaga ataaaaatac aataatttcg aataatcatg caaaggggtg tgccgtggta
     1561 aaggaacgta aaaccgagct ggtcgaggga ttccgccatt cggttcccta tatcaatacc
     1621 caccggggaa aaacgtttgt catcatgctc ggcggtgaag ccattgagca tgagaatttc
     1681 tccagtatcg ttaatgatat cgggttgttg cacagcctcg gcatccgtct ggtggtggtc
     1741 tatggcgcac gtccgcagat cgacgcaaat ctggctgcgc atcaccacga accgctgtat
     1801 cacaagaata tacgtgtgac cgacgccaaa acactggaac tggtgaagca ggctgcggga
     1861 acattgcaac tggatattac tgctcgcctg tcgatgagtc tcaataacac gccgctgcag
     1921 ggcgcgcata tcaacgtcgt cagtggcaat tttattattg cccagccgct gggcgtcgat
     1981 gacggcgtgg attactgcca tagcgggcgt atccggcgga ttgatgaaga cgcgatccgt
     2041 cgtcaactgg acagcggtgc aatagtgcta atggggccgg tcgctgtttc agtcactggc
     2101 gagagcttta atctgacctc ggaagagatt gccactcaac tggccatcaa actgaaagct
     2161 gagaagatga ttggtttttg ctcttcacag ggcgtcacta atgacgacgg tgatattgtc
     2221 tcagaacttt tccctaacga agcgcaagcg cgggtagaag cccaggaaga gaaaggcgat
     2281 tacaactccg gtacggtgcg ctttttgcgt ggcgcagtga aagcctgccg cagcggcgtg
     2341 cgtcgctgtc atttaatcag ttatcaggaa gatggcgcgc tgttgcaaga gttgttctca
     2401 cgcgacggta tcggtacgca gattgtgatg gaaagcgccg agcagattcg tcgcgcaaca
     2461 atcaacgata ttggcggtat tctggagttg attcgcccgc tggagcagca aggtattctg
     2521 gtgcgccgtt ctcgcgagca gctggagatg gaaatcgaca aattcaccat tattcagctc
     2581 gataacacga ctattgcctg cgccgcgctc tatccgttcc cggaagagaa gattggggaa
     2641 atggcctgtg tggcagttca cccggattac cgcagttcat cacggggcga ggttctgctg
     2701 gaacgcattg ccgctcaggc gaagcagagc ggcttaagca aattgtttgt gctgaccacg
     2761 cgcagtattc actggttcca ggaacgtgga tttaccccgg tggatattga tttactgccc
     2821 gagagcaaaa agcagttgta caactaccag cgtaaatcca aagtgttgat ggcggattta
     2881 gggtaagcga tgaaaatcgc cggatgcggc aggcgtaaca ctcgtgcgtc gcatccggca
     2941 attacgttta tccccgcgaa ctaaacaacg ccgccagacc actgcgccgc tcagtacgag
     3001 tggcgattgc cgcacttaat atacgctcat cggcatacag cgacagacgg cgacgcgcgc
     3061 gggtcaccgc ggtgtatacc agctctcgcg ttactatcgg cgtgcgttgg ctcggtaaaa
     3121 tcaacgccgc atggtcgaac tccgatccct gcgatttatg taccgtcatc gcccacgtag
     3181 tttcgtgctc tggcaagcga ctcggttgca cagacttaat attgccgtcc ggcatcgcaa
     3241 accagacgcg cgtcccctgc ccgcgatcca gcgcaatacc gatatcgcca ttaaacaacc
     3301 caagcgcgct gtcattacgg gcaatcatca ccggtcgacc ttcgtaccaa cgagagtgcg
     3361 aatgacgatg aattttgcgc ttctgttgca taaactgctc aattcgctca ttcagtccag
     3421 ccacgccaaa tggcccttcc cgcagggcgc acaaaagttg gtactcattg aacgcctgaa
     3481 tgattaaatc cggctcggca cgcgcttgca gcagatccag ataacgtcca taacccgcaa
     3541 gagcttcctc aagcatcgca atataatctt cgccgctctg taaaagccgt ttttcaatat
     3601 cagtaaaatc ctgctgaaaa acggttttca ctgccgtttt atcaccacgg ttgatcgccg
     3661 cagctaactg accaatgcca gaatcgctgc cgaaacgata gcttttttgc agcaggcaga
     3721 gactgtcgcg caaagatgcc gcttctgtgc cggttcctgc cggaacgtga gttcccgtca
     3781 ggcggcttag ctgcccggca cgctcggcgg taaagccggc attggcataa gcgcagatat
     3841 cgcccagcac agccccagcc tcaaccgagg ccagttgatc acgatcgccg agaaagatca
     3901 ctcgcgcatg atcgggcaag gcgtcgatca gtctcgacat cataggcaga tcgatcattg
     3961 acgcttcatc taccaccagc acatcaagat gcagcgggtt accggcatga tgacgtaaac
     4021 gctggctacc cggctgcgcg cccagcaatc ggtgcaaagt gctggcatct tccggaatgc
     4081 gtttcttttg ttcatcggtc agcggtaact gtcgcaaagc cttgccgaga gattcggtta
     4141 agcgcgcggc agctttaccc gttggtgcag ccagacggat acggcagcgt tcgccgtcgg
     4201 ccatttgaat taacgctgcc agcaacttcg ccacggtggt cgttttaccg gtgcctgggc
     4261 cgccggaaat caccgagatc cgccgcgtca gcgccactgc tgccgccact ttttgccagt
     4321 taatttcatc gcttactgga aaaagtttgt ccagggtttg cgccagtaga gcttcatcaa
     4381 cctcaatggc atgattcacc tcattgaaga agcgcgctac cgtccgctcg ttacaccaca
     4441 tgcgattcaa ataaagacga tcgccacaga ggatcatcgg cgtgggttca tctcccctgc
     4501 tgaccgcttg agaagccagc aaggattctt cccaattttg tagctcaccg atttcactga
     4561 cacaggtcgc caacagcgga tgcgacgcct cgttattttc cagtcgtgaa agcggcaaac
     4621 agacgtgtcc ctctccggca tcatgactta acagtgccgc cgcgagggtg acggcaggat
     4681 gttcatctcc cgccacggtc agggcaaatt gcacatccag cgggcgtagc tgtttgtgct
     4741 ccacagcttc cagtaattgc ttttgcaatt tcattacgcc tcctccaggg tcataccggc
     4801 aaacatctca tccatcaggg caatcaaccc ggcgttgggt cgggttgtgt aaatcccttg
     4861 ttgcggatgt tctttatcaa cgccacgcag gaacagatag ataacgccgc caaagtgacg
     4921 atcatagtcg taatcagcaa tgcgatggcg cagatagcga tgcagcgcca gggtataaag
     4981 ctgatattgc agatcatagc ggtgtgcctg catcgctgct gccatagcct gttgggtgta
     5041 agccgaactg tcttcaccca gccagttgga tttatagtcg agcaggtaat aacgcccttc
     5101 gtggcggaat accaggtcga taaagccttt taacatgccg cgtacctgca tgaactccag
     5161 cggcgggcag ccagcggata gcgggtcaaa ctggcgaatt agtgcatcaa gctggctggc
     5221 gatgagcggt tcgctaatcg gcaggtaaaa ctccatctcc acctgtttat cgcggtcgga
     5281 aagctgactc aggctaacac ccgtttcatt gagaggtgcc tggaggacag ccgtgatcca
     5341 ctcggtcaac accggttccc actgcgattc aaagccgccg agttccagtt tttcctgtac
     5401 ccagttcggg tcaaccggct gggtaaaatc gaggtcttca aacaaactgt gcaagaatgt
     5461 ccccggtgac gcaccgcgcg ggaactgatg cggtgttaac gtcggttctt caacgacgct
     5521 gaccacgcct gcggcatcaa catccagccg tggcatcaga tcctgagcga taccgtgacc
     5581 acgctgctgc aagccggagt agctggtgac acgccagtta tcgccgggca atcgttgtag
     5641 tgtcctcgca ttcagttctg cagttaaagc atcattaacc tgccagggct ggttatcacc
     5701 agtttgtgcc gtttgccagg caatatcatc atcgcataac gcttcaatac aggtgcgaag
     5761 ccctgccgca tcttgcggtt cccctttttg cagcaaacgc ccgagcgcac tttggtggac
     5821 gtcggtgtca ccttttttat cgccacgacg gcgcaccagc ggtgcaacgc cgagactgca
     5881 atgccaaacc gaacgcgtca gcgccacgta aagcaaacgc agatcttccg ccagacgttc
     5941 gacctccgcg aggtcgacgc tttctggcgc agcattaaga tccaaaactg cctcaaacga
     6001 gtggcgatcg tgataaaacg cctgatcctg gacgcggaaa ttggtaataa atggcaacca
     6061 gaccagcgga tattccagcc ctttcgattt atggatcgtg acaatctgca ccagatgttt
     6121 atcactttcg aggcgcattt gttggctgga ggcattactg tctggctcga ggatatgttg
     6181 cgataaccag cgtaccagcg catgttcact ttccagctgc gttccggctt cttgaagcag
     6241 ttcgctgata tgcaagatat cggtaagacg ccgctcaccg cctgccgttg ccagcaagtt
     6301 ttctgcaatg ttacgcgccg acatcagcgc ccgcagcatc ggcataacgc cacgtttgcg
     6361 ccagatttgc cgataaccat cgaactcttc gactaccgca tcccacgcat gttcgtcatt
     6421 attcagcgtt tcaatatcca gcgcattcag ccccatcatt gacgttgcca gcgcactgcg
     6481 cagggtgttc tcacgttcgg gcgtcatcac cgcctgcaac agccaaagca tttcctgcgc
     6541 ttccagagtt tcaaaaacac tatcgcggtt cgaaaggtaa acggaaggga tttccagcaa
     6601 cgttaaggca tcgcgcacct gggcggcctc ctggcggctg cgcaccagca cgctgatgtc
     6661 cgaagcacgc accggacgcg cgtcgtcgcc gttcatcagc aacgcttcgc cccgctgtcc
     6721 ggcttgtagc cagtcgcgga tttgcgcagc acatacctgc gccatggtac tttgatagtc
     6781 gccaacgccg cagctttcgc cttccatcag ccacattttc atcgcaggct gcgtttcacc
     6841 tttaaagaca aaacgtaacg cctgattttt cccggctgat ttcactggaa taaacggtat
     6901 ttcgcgaaac atgaacgcgt catcagtctg gctgaaaagc ttgttcacac tgttcaccat
     6961 tccaggtgcg gagcgccagt tggtgtctaa agtgtagtgg gcgtgaactt cactacgcgc
     7021 cttcatataa gtgaagatat ccgcaccccg gaatgcatat atggcctgct tcgggtcgcc
     7081 aatcagcaac aatgcggttt ctggctgatg gtgccagata cggcgaaaaa ttcggtactg
     7141 ctgggggtcg gtatcctgaa attcatcgat cattgccacc gggaatcgcg tacggatcgc
     7201 cgctgccaac acctcgccgc tttcgctacg cagcgcggaa tcgagccgac ttaacatgtc
     7261 atcaaaaccc aattcgccac ggcggcgttt ttcacgcgct actgtttcgc ggatctcagc
     7321 caatgcgcgg gtgatcacca gatcacggat cgacaatggt tctgcaagca gttgatcgat
     7381 cgcctcaaac agtggatgtc gcggggtttc ccccccggct ttcgtgcgat cttctaagaa
     7441 acgctgggag aatttttcca gcgactccgg caactgataa ctgtttgtct cttcttctgc
     7501 ccaggcgctg atcttgtcga tccatttagc ctgattgcta cggttaaact tgcgtcgatc
     7561 aataccagaa gattcgatca gcgcatccag ttcacccact gcgtcgcgcc actgctgttt
     7621 taccgtatca atacgcgcca caatttgcgc gtgacgggaa gctagcgttt catcatcggg
     7681 cggcggtgct ttgataaccg gcgcttcgcc ttgcagataa cgattaatat cgcgcagcaa
     7741 cgcctgcggc cctttccagg tttcaaagac gacctgggct atttcacgcg gcagcgggta
     7801 gcagtggcga cgccagaaat cggcgcaggc ctggtagcgt agcagagact catcttcaat
     7861 cagctgctgc tcaaacagca tgccggattc aaaggcattc aggttgagca tgcgctggca
     7921 aaagccgtga atagtaaaga ctgccgcttc atccatctgc cgttcggcta acaacaacca
     7981 ctgcgcggct tgcgctttat cgtcgacctc ttccagcagg tgttcgtaca atggattgtc
     8041 ggtggtttca cgcagacagg cgatgcgcaa ctcgtggata ttgctacgga tacgaccgcg
     8101 caattctgcc gtggcagcct cggtaaaggt gaccaccagc agttcttcaa cggtcagcgg
     8161 gcggggaaag gcggcggaac cgcctagtcc aagtaacagg cgcaaataaa gcgccgcaat
     8221 cgtaaaggtt ttgcctgtgc cggcagaggc ttcaatcagg cgctcgccct gtaagggcaa
     8281 gcgcaaagga tctagtgtct cggcgacatc actcattctt ttcactcatc aggggcattg
     8341 tttgctgcaa cgcgctgacg ttctcccaca ctttccagcc ttcaggatgc acatattcgg
     8401 ctttcccgtt ttggctgccg gaaatctgcg acaggatagc catgccttgc ggctcgacta
     8461 ccgcctgatg gaagaaatcg gccagttttt gcggcgtcag cagtttaatc tgggccacga
     8521 ttttatcacg cgaatcgaag cgcatattgc cgcgatcgaa atctttactt aacttcgatg
     8581 cttcttcgcc gagcgtttgc ggtgcctgca gcatctgggt aattaccgcc tgctggattt
     8641 gcgcaaactc atctggcttc atcgctcgca attttgcctc tgcggttggg aaaaacgcct
     8701 tgtaacgctc ccacaagaat gaaggctgtt tatcattgct ttgcaaaagg aagcccatgc
     8761 cccactgacg ccccacgctc attggaaacg caaacacggc atagcccaat tgttcttcgg
     8821 tacgcaactg attgtagaac cacggctgta cgatctgccc caacagagag ctataggctg
     8881 agctggtgta ttcatcgtag ccagtcggta caaataccgc tgccagtgcg gagtcggtgc
     8941 tgttaccggc tttttcaaag atgacggatt gttttttatc gaccactaca tctttgtttc
     9001 gacaccactc tgaaccatca gcgcccaact gtttttgcac atcgcgtgcc agcgttgttg
     9061 cctgggcctc ggtcatgttg ccgataacca taaactctgg tcgagcccct gattttaagg
     9121 cgtcgcgata agccagcacc tctttcaacg taatggaggg caaaatttta cgccgttcat
     9181 ctcgcgagaa gtacggcact tgcgagagca tctgcgcggg cataatcgcc tgctcaaacg
     9241 ctttaccctt ttctgcggaa tccatcatct ggttatacca ggacttcgcc tgctcaagct
     9301 gatcttccgt agcggtatag ctaaagtacc cctcgagcaa tgcctggaac agctgcggca
     9361 gacgctgggt gtaaccatta gcattaacca taaggccgtt gttagcgttg gtggaaaaac
     9421 ttatgccacc aaccgacgcc tggttgctta actgatcaag cgccagccct gcgagataat
     9481 cattgagcgc aaacatcacc tgattgcggg cgctgtccat ggctttcgga ttacgcaaaa
     9541 tcaggctgac atcagctttg ggttcgctgg aaaaataacg gcttggcgca tacaccacgc
     9601 gcagattcga ctcatcaaca atcagctctg gatggtcata tttcttctct gacttaatca
     9661 gcgagaaatc gtcaggaata taggggttaa gctccggtaa ggagagcgca atattggcag
     9721 ctttttgctg ccagtcagcg aaagtttgtt cgctgatttt atcgacctga tacggcgcat
     9781 cgacaaaata agccgtttta ttgtgcggct ctttcgggct gatataccag atacgcgcat
     9841 tctgcggcgt catcatcgcc agacgttctt ttactgcttt agcatcgtac cgatcggcaa
     9901 tattgactgc atccagcgta tgctcaaccg gaacgcgaat catggtatct gccagccatt
     9961 cgacgtaatc catatcacgg gtgatcgacg gataacggaa gtcgatatcc agcacattcg
    10021 ccagttcatc gaagtattgt ttatcaatgc ctttttcacg taacagattg agatagctaa
    10081 aaattgccgc cacaacctga tcgcgattag ccaggccttt atcggttaaa gacgcagaga
    10141 tcgctaatac gccgctgttg ccgttgacga taggatcgga gttggcgcta atgccctcaa
    10201 ctaatccctg cttttgcagc cagtcggaaa gtgtacctgg gctgcgatta ccaatcagat
    10261 aggtaatcaa ttcatccgtt ttactacgga acttcgctga gttgttatcg atgcgaaact
    10321 caacgcgcag aactttacgc ggcattgccg ggacgtaatg aatgataatg cccttttgcg
    10381 cgtcggtgac taccggcacg gtgatttccg gttttttgct ctctttgttc ggcacgcgac
    10441 caaaggtgtc cgccgccatt tttgccaact ccggcagcgg tttattgctg taaataaccg
    10501 ccttcatcag attggcggaa tagtacttct cgtggaaatc tttcagcgcc tgctgcaccg
    10561 gattaccagg tttatcgctt aaagtttcga ggttaccacc agaaaacttt gaaccggggt
    10621 gtgccgggtt aatggtttct gcgctgacct gtgccatgcg catcccgtca cgcgtacgcg
    10681 ccatggttaa ttcagcgttc accgcattac gctcacgttc ggcatatttc ttgtcgagca
    10741 aaggttcagc aatagcatcg gccaggcggt ctaccgcacc aggcaaggcg tcgttctcaa
    10801 cttccagata gaaagccgtg cgatacggcg cagtgctggc attgtgacta ccgccgtgca
    10861 ttttgagata ttcggccaga ctgtcagcct gcgggtactt tttcgacccc atcagactca
    10921 tatgttcaag gtaatgtgcc agcccctggt acgcctcggg atcttccagc gacccaacgg
    10981 gcaccaccag cgccgagagc gatttaactg cctgcggatc agaaaccagc aagaccacca
    11041 taccgttatc cagacgtata gcctgatact ggcggttatc tttatcactt ttacggatgg
    11101 tttcctgaat cggctgccat cccgtttctg cctgacttaa gggtgcccaa agggcaacta
    11161 acaacaataa tgctttgaac caggtgctgc ggggcattct cggacctcat aatcaactta
    11221 attttctgtc cagattcaac acgttaacgc aaatcatctg ccagaattta atcttgtgct
    11281 gcacgagtca gcctatgttt atataaccat cagtccgtga ctggtgcgca tcataaagta
    11341 agcggataga ttgcgcaatt tttatacagc actcatgact gattaaagcg aaacagcggt
    11401 aacaggaaac gttgcgactg ttcaacgatg gcctccattg tctctggtgt taattgccgc
    11461 cagagccttt gataccagat atcatcacct tcgccacgca ccatcatgtt accttcgtaa
    11521 gcctgaagga atttcgtacg ggctttttgc aacgtggaat cgtcatccag catggcatcg
    11581 ttttgcgcgt cataacaggt ttttagccac gcgccgccac tttcaggtaa caccagcaat
    11641 ggcgcggaca tcccttcacg atacccctca atcagttgtg agaggtaatg caaagcctgt
    11701 tcggctgcaa gcggcggaaa acgccactcg ccgtctttgc gtagaaaaag gcgactttca
    11761 ccattaccac cgctggcaca gtagacaagg tgttccagcc aaagttgcat tccctgcgcc
    11821 acacttaata aagagggacg ccagcgcaac aggccatccg gctgcacctg cggcaaccag
    11881 ccagttatct gcacaccgtt gcaggcgaga tcaatttcca tactctgccc cggctggcga
    11941 caggcaatga ctctgtcggc aagctgctgc atctcctggc actgagtttc ccagaaaatt
    12001 tcaccaaaag cgccatacgg taaatcccct gccgcccgga agcgacggaa caagcgttcg
    12061 gcatcatcct gctcaaccag tgcattcaat aactgctgat tgatttgata acggctaagc
    12121 ccttccagaa taaatggctc ggtgtcgggg atttcgctgt cttcagtacg gaagttcacc
    12181 tgcaaacgca tctggaagaa cgcccgcacc ggatgtgccc agaatcgttg tagcgtttcc
    12241 agcggcacgg tttccggtaa ggtaaacggc agcggctgaa caaattcaga atgtgcttta
    12301 ccagcctggc tggccgcagg tagccattca cgagcatagc tttgtcgttc gcctggctga
    12361 tagttttgtg gatcaaacgg cattcgggta tggaggcaag taagatgcgc ttttaccctt
    12421 gcctcgcttt catcacagtt gagcgcttca tcgcccggta gataatgact ttgcccgatg
    12481 tagtcgatca gttcctgcac cagtaccgac gggaaacgtt cactgttatc ctgaatggaa
    12541 cgcccgatat agctgatata gagtttttgc tgcgcggaaa ttaacgcttc caggaacaga
    12601 tagcggtcgt catcgcgacg gctacggtcg ccacgcttcg gtttctggct catcaggtca
    12661 aagcccaatg gcgcaagctg acgtggataa acgccgtcgt tcattcccag caggcaaacc
    12721 actttgaacg gaattgaacg cattggcatc agagtacaaa tgttaaccgg tccggcgaga
    12781 aaacgctggc tgatacgttc ttgatccaga cgctgtgcca gttcatcacg caatagtgac
    12841 agcggcaccg cgtcgccata ctgcgcacct aaaccttcgg cgataatcgc ctgccattgt
    12901 tgttcgatca gcgtcatcgc cgcttcggtt tccgcatccg gcaggaagaa ggcgctgagc
    12961 atatcgcgac aaaccggcaa ccactcttct agcggacgct cctgtgccag cccgcgacgc
    13021 cagatgttta gctgcattag cagtgaagcc agatgcccca ccagttctgc aattaagccg
    13081 ctcgattcat cataaggtag aaccgattgc cactcgccct gcgcgctctc catcgcgtag
    13141 cccagcaaca tacgcgtcag gccaaatcgc caggtgtgtt gtccggtggc ggggagttcc
    13201 agctcgcgaa cgttgtcgtc atctatcccc caacgaatgc cggattcgtt gacccactgg
    13261 cgtaaataac gcagcccttc ttcggtgatg tcaaaccgcg ccgccagcac cggcacatcc
    13321 agtaatgcca gcacgtcttc cgacacaaag cggctgtctg gcaacgataa caggctgata
    13381 aacgcttcca gtaccggatg cgactgccgc gcccgacggt cggaaatggc gtaaggcagg
    13441 taacgatccg caggtgcgct accaaacaca gcccgaataa acggactgta gctgtcgata
    13501 tcagccacca tcacgatgat gtcgcgcgga gtaagtgtcg ggtcttcttc cagcatcgcc
    13561 agcaggcgat cgtgcaaaac ttcaacttca cgctgcgggc tatggcaaac gtggaaggtg
    13621 atactgctat ccagtggatc aagcgggcgt ttgttatcgc tacgggaaaa ctcttcgatg
    13681 ttcacaccag caacggcacg gttttccagt tccagaatgt cagactgaat gttatgcagc
    13741 aggttatctg gcgtgacatc gacaaaggcg tccagctcct ggctgctctc caggtcagaa
    13801 aggagataaa tgtagtcgcg cccaagctta ccccatgaag ccagcagcgg gttgccgaca
    13861 tcctgttcac catcgctgtt aaagagctgc ccggcatttt cgctatcacg aaatagcggc
    13921 aattcgcgat cttcaaaact gtgtcgacgc tgacgggtca gcagtttcgc cagataagca
    13981 ggatctttaa tatcgcccca gtaataacgg caggggttgg taaacaggag atggatttca
    14041 atatgtttac ccagcgcctg tagcgcctgg agataaacag gcggtaacgc ggaaataccg
    14101 catataaaga cgcgcgaagg taaccccggc gggcaggtcg tcgcggactc cagcgtttcg
    14161 ataaagcgct gatagagatt ggcgcggtgc cagcgcggtt gcccgagttg atgggtatat
    14221 tccaccagcg ccttccacaa cggggcttgc caggcctgtg cttctcccag cccttcaacc
    14281 aaatgtcctg tttcccactg tgccagccag tccggacgat agaccagata ctggtcaaac
    14341 aggtccgccg cttttgagga aagctggaac agttttcgct tgtcgctatc gtcagtcaga
    14401 taatgccgca acagggtaaa gtcttcgcgc tccagcaatt gcggcagcag agtcatcagt
    14461 ttccagctca tgctctgttt gttaaaggcg ctctctttgg ggatttccgg taacacccgg
    14521 acgaacatat cccagataaa gctcgctggc agcggaaaat caatgtttgc cgcaatacta
    14581 aacttttgcg acagggtcat ttgcagccac tgcgccatac cggtactttg caccagaatc
    14641 atctctggtt cgaaaggatc gtccagccgt tcgcgttcga caataaactc catcaacgct
    14701 tccagcacgt ccagacgatt ggaatggtag acccttaaca tagcggctcc tgactactga
    14761 cgattcgggc aatgcaggcg ggtcatctcg ccttctctgc ccccgggtga aactagcgta
    14821 acgctgatgc tgacacatcc cgcctgcgat gtctgcattc ggttgacctg ccagttggca
    14881 ggtggcgaaa tcgcgcgcag ttgcgtttgc tgccaaccat gccgccagag ctgttggtac
    14941 tggtttctgc tggcgagact gttcattaat gttcgctgga tacccgataa cgcagtgaca
    15001 atcatcacca tcagcaccat cgcaaccatt acctccggca ggctaaagcc ttgttgattc
    15061 ttcagggaag ctgacataac gccctctctt tcagcggaca aaaatcgctc cagccgcgtg
    15121 gcgaaaaaac aatgtttcca tcaatgactt cgcctgttcg ccacaacgaa acgccttcat
    15181 aaccggcaac caataaggct tcattatctg ccagtaaacg caaacaaacc cgggcatcgg
    15241 ttccagcgta ctgcgagcac tgaactgcgg gctgcgtctg ccaggagtgc attttccccc
    15301 acgccagcgc cgactgaacg atggcctggc ggcgcaatga ctgactttct atgctcacgc
    15361 gagaggcaaa actgcgatcc tgctgactca ttccttgcaa tagcaagcta cccaaaacca
    15421 gcagcatcag gaccagagcc agtgacgaaa cacctttttc gcggttcaca ggttgaatcc
    15481 tgtcacgcta tagctggcat ccaccacggt ttgcggttca gacttactgg cagcacgcat
    15541 attaaccgtc aacaccggcg agaagccgct gacatcctga cgtacgacct gaaaagtgtc
    15601 gataatgatg gcatccggat tagtgacttt atcccagccc ttaccttcac aggatgtcgc
    15661 accgcgtagc gtttccagca catgctcctt cagacgaaat ccaatctggt cagactcttt
    15721 taccggttcg cgatcccaga taccgttact gttcgcatcc cactgcacaa tgatacagtc
    15781 accctgtccg acaatttcca gcccttcgcc ggtacagctg ccatgacaat aacccgccct
    15841 ctggagatgc ttcgcgacgg taaatacccg cagccagatt tcatcttcca gcgccagctt
    15901 acgggtgctc gttaaacttt cacgctgtaa cgcaggcaga aagcgtgcag cccccagcaa
    15961 caatacgcta ctgatcgcca tagcaatcaa cacttccagc agagaaaaac cttgctcttt
    16021 tacaggcatc cttctgtttc tccttgctga caaagccgga gtcttcccca cggcgaaacc
    16081 accagccacc actcgcccgt tgagtttttg aagcgaatat gcccggccca tgcggtattg
    16141 cgcaggccaa agaaagcaag cgaaggtgtc aggtcgctca tttcgacttc gggccagcgt
    16201 ggcacaaaga ccaatggtga actgccatga caggtattgg ccccagcagc ggaactcaca
    16261 aggcaccata acgtcccctc cctgataacg ctgatactgt ggtcgcggtt atgccagttg
    16321 gcatcttcac gtaaatagag caaatagtcc cgcgcctggc tggcggtttg ccatagccgt
    16381 tgcgactgct gccagtattg ccagccatag agtccacttg cgcttagcat gaccaaaatc
    16441 agcatcgcga ccagcgtttc aatcagcgta taaccacgtt gtgttttcat gccggcagta
    16501 tggagcgagg agaaaaaaag acgagggcca gtttctattt cttcggcgca tcttccggac
    16561 tatttacgcc gttgcaggac gttgcaaaat ttcgggaagg cgtctcgaag aatttaacgg
    16621 agggtaaaaa aaaccgacgc acactggcgt cggctctggc aggatgtttc gtaattagat
    16681 agccaccggc gctttaatgc ccggatgtgg atcgtagcct tcaatctcaa agtcttcgaa
    16741 acggtagtcg aagatggatt cgggtttacg tttgataatc aacttcggca gcggacgcgg
    16801 ttcgcggctt aattgcagat gagtttgatc catatgattg ctgtacaggt gcgtatcgcc
    16861 accggtccag acaaaatcac ccacttccag atcgcactgc tgcgccatca tatgcaccag
    16921 taacgcgtag ctggcaatgt tgaacggcag gccgaggaag acgtcacagg agcgctgata
    16981 aagctggcaa gagagtttgc cgtctgccac atagaactgg aagaatgcat ggcacggtgc
    17041 cagcgccatt ttatccagtt cgcctacgtt ccacgctgaa acaataatgc ggcgggaatc
    17101 cgggtcgttt ttcagctggt tcagtaccgt agtgatctgg tcaatatgac gaccatctgg
    17161 cgttggccag gcgcgccact gtttaccata cactggtccg aggtcgccgt tttcatcggc
    17221 ccattcgtcc cagatggtga cattgttttc gtgtagataa gcaatgttag tgtcgccctg
    17281 cagaaaccac agcagttcgt ggatgatgga acgcaggtgg caacgtttag ttgtcaccag
    17341 cgggaatcca tcctgcaggt taaaacgcat ctgatgacca aaaatggaaa gcgttccggt
    17401 tccggtacgg tcgtttttct gtgtgccttc gtcgagcact ttttgcatca gttctaaata
    17461 ctgtttcatg gttcctcagg aaacgtgttg ctgtgggctg cgacgatatg cccagaccat
    17521 catgatcaca cccgcgacaa tcatcgggat ggaaagaatt tgccccatgc tgatgtactg
    17581 cacccaggca ccggtaaact gcgcgtcggg ctggcggaaa aactcaacaa tgatgcgaaa
    17641 cgcgccgtag ccaatcagga acaagccaga gacagctccc attgggcgcg gtttacgaat
    17701 atacaggttg aggataataa acagcaccac accttccagc agcagctcgt aaagctgtga
    17761 tgggtggcgc ggcagcacac cgtaagtgtc gaaaatggat tgccactgcg ggttggtttg
    17821 cagcagcaaa atatcttctg tacgggagcc tgggaacagc atggcaaacg ggaagttcgg
    17881 gtcaacgcgg ccccacaatt caccgttaat aaagttgccc agacgcccgg caccaagacc
    17941 aaacggaatg agtggtgcga taaaatcaga aacctggaag aaggaacgtt tagtacggcg
    18001 ggcaaagata atcatcacca cgataacgcc aatcaggccg ccgtggaaag acatgccgcc
    18061 gtcccagaca cggaacagat acagcggatc ggccataaac tgcgggaaat tgtagaacag
    18121 aacataacca atacgtcccc cgaggaagac gccgaggaag cccgcataga gtaagttttc
    18181 aacttcattt ttggtccagc cgctgcccgg acgattcgcc cgtcgtgttg ccagccacat
    18241 tgcaaaaatg aaacccacca gatacatcag gccgtaccag tgaagcgcca cgggtcctat
    18301 tgagaaaatg accggatcaa actccggaaa atgcagatag ctactggtca tctgtcacca
    18361 caagttcttg ttatttcgct gaaagagaac agcgattgaa atgcgcgccg caggtttcag
    18421 gcgctccaaa ggtgcgaata atagcacaag gggacctggc tggttgccgg ataccgttaa
    18481 aagatatgta tatgatccgc gctataaccc tccgcgaatc agcccgccca tgccgcgacg
    18541 ctccataaag gctgcaacct gatggcgaac ttcggtcgcc agttgcgctt ccagactacg
    18601 ctgcgcaaga ttttctgctt cggcataatc aatgcgccgc agcaggtatt ttacccgcgc
    18661 tacagaacgt ccgttcatag acagatggcg atacccaagc ccaatgagga ttgccacgca
    18721 catgggatcg cccgccattt caccgcacaa acggagatcg attccatgta tttccgcttc
    18781 ccgggcgatc atcgccagag ctcgtaacat tgcaggatga agactgtcat aaatgttcgc
    18841 cacccgggta ttgttgcgat caacggccag aatgtattga gtcagatcgt tggtgccaac
    18901 agagatgaaa tcgacccgct ttgccagatg cggcagcata aataccattg acggcacttc
    18961 cagcatgatg ccgatacgtg gtttgggaat ttcgtaaccg atcatctcct cgacttcacg
    19021 tccggcacgt tcaatcaggc ggcgtgcttc atcaacttca tcgaggcttg tgaccatcgg
    19081 caacagaata ttcaggttgc ccgtagcggc attagcacgc agcatcgccc gcacctggat
    19141 caagaagatc tccggctgat cgagcgtaat gcgaatccca cgccaaccca ggcatggatt
    19201 ctcttcgctg atcggcatgt aaggcagctg cttatctgct ccgacatcca gcgtacgcaa
    19261 ggtgacgggt ttatcattga acatttgcag catcccctga tactgcgcca cctgttcttc
    19321 ttccgacggg aaaccacttt gcagcatgaa tgggatttca gtgcgataaa gaccgatgcc
    19381 atcaatacgg ctgcccagtt tttcttcatg ttccgggctt aaaccagcat tgagcatgac
    19441 ttttatacgc tcaccgcttt ttaactgcgc gggtaaattg acgtcatctt ccgccagacg
    19501 gctaagctca atctcttcac taattagccg ctgatattct tgcagcagta ccggctccgg
    19561 atcgaccagc aattcaccgc gatagccatc aacgatcagc gtcctacgat gcagcaccga
    19621 aggctgaata tccgcgccca tcacggtagg gatccccagc gcacgtacca tgatcgcagc
    19681 atgggagttg gctgcgccat ctcgcacgac aacaccgact aagcgatcct ggggcagctc
    19741 agcaagagtt gtcgctgaca gttcatctgc caccagaatg aaacgttccg gccaggcgtt
    19801 cggcccttga ttagcgtcat caagatgaaa cagcaatcgc tgacccagcg cacgtaaatc
    19861 gccagcccgc tctttgagat agttatcgct tagcgcggca aactgttcgg caaatttttc
    19921 aatgaccgtt tttaccgccc actctgccac cgagccttta tcaacctcgg caaacaattc
    19981 gcgacgcagc cgggtgtccg aaagtaggtg cgagtaaaga tcgaaaatag ccgccgtttc
    20041 tttttgtgct ccggcggcaa agcgtttgct gtagcggcga aactcgtttg ccgcttcttc
    20101 cagcgccccg gtcagtcgtt cgcgttccag agccggatcc agcgttgatg cctgatacac
    20161 ctgttccatt aaaggtaacg tggcatcctg ccagccttcg gcaatcgcca caccaggtgc
    20221 tgccggtaat gcgcggattc gcgtctggcg atattgccca aacaaggcag tcaactgcga
    20281 ctgagaaaga atagctgcca tctgggtggc aagcgtcacc aggaaggatt cttcactttc
    20341 gtcatactgg cgcaactctc gttgctgtac caccagtaca ccaagcaact ggcgacgttg
    20401 aataattggt acgcctaaaa acgcgcggaa acgttcttct tttacggagg ggatgtattt
    20461 gaagctgggg tgcttttgcg catctgcaag gtttatcggt tccgccagcc tgccaaccag
    20521 gccgacgatc ccttcatcaa acgcgagcgt tacagtgcga ccgcgtggtt ttttcagccc
    20581 ccgggtcgcc atcaggtagt aacaacgtcg atcatgatcg gccaggtaga ccgaacagac
    20641 ctcggtatcc atcgcaagac agatgtcggt aaccagaata tttaacgcct cattcaggcg
    20701 tggtgcgctg gctacctttt cgactatttc gcgcaggcga gtgagcataa ttggcgtgac
    20761 ttaacctctt ttacgtcgat aagcagatgt gttttgtggt tttggcgtat tttcctgcag
    20821 tgacatcacc acactcgcga actctttcat taccctacgg tagacatcac gtttaaatga
    20881 caccacctgt ctgaccggat accagtaact tacccatcgc cagccgtcaa actctggtgt
    20941 actgctggtt tgcatattga tttctgcatc gccgctcacc agctgcaaga gaaaccattt
    21001 ttgtttttgg ccgatacaaa ccggcttcgt gtcccaacgc accaaacgtt tcggtaattt
    21061 gtagcgcaac cagttacgcg ttgaagcaag gattcgaacg tctttgcggc ttaatcctac
    21121 ttcttcaaac aattcacggt acatcgcctg ctctgcggat tctccggggt tgattccgcc
    21181 ttgcggaaat tgccaggagt gctgaccaaa tcgccgggcc cacattacct gcccctggcg
    21241 attacaaatc acgataccta cgtttgggcg gtagccatcg tcatcaatca ccggactacc
    21301 tcaaaataaa gctttatata cgaatgattg tttcatactc caagaagacg gtaaaccact
    21361 ctctgcaggg cattacacac taataacaat tgaataactc acagttatgt gcagagttat
    21421 aaacagagga aggggtggat agccgttttt attcaccttt tctgtggata gagttgtgaa
    21481 gaactacgga attactacgg gaaaacccgg agcattctga ataagcccga tatagacatt
    21541 ttaaaatatt cattattagt caatgcatta catgtaattt gagtaatgaa aaagagtaat
    21601 tcgtgaccca ggtcacacct ctcatttacg ggttggcgaa agatcaacca atgccgtatt
    21661 tatccacaga atgtgccact aagttaagca ctgaaccact aaaaactgga gtttcgtcgc
    21721 acgtcaaggc tgtaaatgga aacagtagtg gaggtttttc acagttatcc cagctttctg
    21781 tggataacat ggtgtaagat cctgtttatt ttcagtgacc agatttggaa aacctattgc
    21841 agtgttgcgc aactcgatta ccggcagcct aaaaagcaat ataaatcaga gaattgaaca
    21901 acacatgtgg aaaaagttac actgcgaata ttcggcacat aattgctgtt tgttttttaa
    21961 ccaaggtatc atgacatgtc ccaacctcgc ccactcctct ctcctcccga aactgaagaa
    22021 cagttgttag cgcaagcaca gcaactttct ggttatacat tgggagaact ggcggcactt
    22081 gccgggctgg ttacgccaga gaatttaaaa cgcgataagg gctggattgg cgtgttactg
    22141 gagatctggc taggtgccag cgcagggagt aaacctgagc aagattttgc tgctctgggc
    22201 gtggaactta aaactatccc tgtggatagt cttggtcgtc cgctggaaac aacattcgtt
    22261 tgtgttgccc cgttaacggg taatagcggg gtgacctggg aaaccagcca tgtgcgccac
    22321 aagctcaaac gcgtactgtg gataccggtt gaaggtgagc gcagcatccc gctggcgcag
    22381 cgtcgcgtag gatcaccgtt gctgtggagc ccgaatgaag aggaagaccg acagctacgc
    22441 gaagactggg aagaattaat ggatatgatt gttctcggtc aggttgagcg gatcaccgct
    22501 cgtcacgggg agtatttaca gatacgaccg aaagcagcga atgcgaaagc gcttaccgaa
    22561 gccattggtg cccggggcga acggattctg acgctgccac gcggctttta tttgaagaag
    22621 aatttcacca gtgcactact ggcccgtcat tttctgatcc agtagccatc gctttgacct
    22681 gccgctttcc gggcatataa ttaccgcttc atttttttgg cagggctttt tagatgttat
    22741 ttgcatggat aaccgatcct aacgcctggc ttgcgctcgg tacgctgacg ctgctggaga
    22801 tcgttcttgg gatcgacaat attattttcc tttctctggt ggtggcaaag ctccccacag
    22861 cacaacgtgc tcatgcgcgc cgtctggggt tggcgggagc catggttatg cgtctggcgc
    22921 tgctggcatc aatcgcctgg gttacgcgcc tgacaaatcc gctttttaca atattcagtc
    22981 aggaaatttc cgcccgtgat ttgattctgc ttctgggtgg cttgttcctt atctggaaag
    23041 ccagcaagga aatccacgaa tccattgaag gtgaagaaga agggctgaaa acacgcgttt
    23101 catcattcct cggcgctatc gtgcagatta tgctgctgga tattatcttc agcctcgact
    23161 cggtgattac cgctgtgggt ctgtcagatc acctgtttat tatgatggcc gccgtggtga
    23221 ttgccgtagg cgtgatgatg ttcgccgcgc gctcgattgg tgattttgtc gaacgccatc
    23281 cttcggtaaa aatgctggcg ctctctttcc tgattctggt cggctttacc ctgattctgg
    23341 aaagtttcga catccacgta ccgaaaggtt acatctactt cgcgatgttc ttctctattg
    23401 cggttgaaag cctcaacctg attcgcaaca aaaagaaccc gctctgatac tccgtacgct
    23461 ctcctgcggg agggcgtact cactcctgtt accctcacag tttaagattt tcctgctttc
    23521 aagatatata acgtcggttt ataaacagac tattatcata ggtgggccaa atgaggataa
    23581 ccggatgaaa aaatgggcag taataatttc tgcagtcgga ctggcgtttg ctgtttccgg
    23641 gtgttccagt gattacgtca tggcgaccaa agatggccgt atgattttga ccgatggaaa
    23701 acctgaaatt gatgatgata ccgggctggt gagttatcac gatcagcaag gtaacgcgat
    23761 gcaaattaac cgtgatgatg tttcgcaaat tattgaacgt taacaaataa ggtcagcatc
    23821 cggctgtaat gccgatcagt taag
//
LOCUS       DAJTZL010000049        23776 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.049, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000049 DAJTZL010000000
VERSION     DAJTZL010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23776
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.049"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            103..1329
                     /gene="eutH"
                     /locus_tag="OO544_001710"
     CDS             103..1329
                     /gene="eutH"
                     /locus_tag="OO544_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HCR6767329.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     CIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVIIVAILVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMK
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKEDATTAKTEAEAQS"
     gene            1326..2729
                     /gene="eutA"
                     /locus_tag="OO544_001711"
     CDS             1326..2729
                     /gene="eutA"
                     /locus_tag="OO544_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HCR6767330.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKTLILEQYQAAGIAPESVDSGAIIITGESAKTRN
                     ARPAVMVLSQSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQL
                     VQVTRRMAELIVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDETDLVSAWQQALLQLDLDPKTDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            2741..4102
                     /gene="eutB"
                     /locus_tag="OO544_001712"
                     /pseudo
     CDS             2741..4102
                     /gene="eutB"
                     /locus_tag="OO544_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
     gene            4123..5010
                     /gene="eutC"
                     /locus_tag="OO544_001713"
     CDS             4123..5010
                     /gene="eutC"
                     /locus_tag="OO544_001713"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HCR6767331.1"
                     /translation="MDQKQIEEIVRSVMASMGQAAPAPSEAKCATTNCAAPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRTGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            5020..5679
                     /gene="eutL"
                     /locus_tag="OO544_001714"
     CDS             5020..5679
                     /gene="eutL"
                     /locus_tag="OO544_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HCR6767332.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            5692..6192
                     /gene="eutK"
                     /locus_tag="OO544_001715"
     CDS             5692..6192
                     /gene="eutK"
                     /locus_tag="OO544_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HCR6767333.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVRQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            6238..7290
                     /gene="eutR"
                     /locus_tag="OO544_001716"
     CDS             6238..7290
                     /gene="eutR"
                     /locus_tag="OO544_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HCR6767334.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEDAQPMVTAESISHQSYRRLLSRAREYVLENMSELVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(7296..8195)
                     /gene="hemF"
                     /locus_tag="OO544_001717"
     CDS             complement(7296..8195)
                     /gene="hemF"
                     /locus_tag="OO544_001717"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HCR6767335.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(8199..9068)
                     /gene="amiA"
                     /locus_tag="OO544_001718"
     CDS             complement(8199..9068)
                     /gene="amiA"
                     /locus_tag="OO544_001718"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HCR6767336.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            9282..9707
                     /locus_tag="OO544_001719"
     CDS             9282..9707
                     /locus_tag="OO544_001719"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HCR6767337.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            9694..10143
                     /locus_tag="OO544_001720"
     CDS             9694..10143
                     /locus_tag="OO544_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HCR6767338.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRATFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            10204..10779
                     /locus_tag="OO544_001721"
     CDS             10204..10779
                     /locus_tag="OO544_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HCR6767339.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            10875..11774
                     /gene="yfeX"
                     /locus_tag="OO544_001722"
     CDS             10875..11774
                     /gene="yfeX"
                     /locus_tag="OO544_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HCR6767340.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(11832..13136)
                     /gene="pbp4b"
                     /locus_tag="OO544_001723"
     CDS             complement(11832..13136)
                     /gene="pbp4b"
                     /locus_tag="OO544_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
                     /protein_id="HCR6767341.1"
                     /translation="MKRTMLYLSLLAVSCSVSAAKYPVLTESSPEKAGFNVERLNQMD
                     RWISQQIDVGYPGVNLLIIKDNQIVYRKAWGAAKKYDGSVLMEQPVKATTGTLYDLAS
                     NTKMYATNFALQKLMSEGKLHPDDRIAKYIPGFADSPNDTIKGKNTLRISDLLHHSGG
                     FPADPQYPNKAVAGALYSQDKGQTLEMIKRTPLEYQPGSKHIYSDVDYMLLGFIVESV
                     TGQPLDRYVEESIYRPLGLTHTVFNPLLKGFKPQQIAATELNGNTRDGVIHFPNIRTS
                     TLWGQVHDEKAFYSMGGVSGHAGLFSNTGDIAVLMQTMLNGGGYGDVQLFSAETVKMF
                     TTSSKEDATFGLGWRVNGNATMTPTFGTLASPQTYGHTGWTGTVTVIDPVNHMAIVML
                     SNKPHSPVADPQKNPNMFESGQLPIATYGWVVDQVYAALKQK"
     gene            complement(13141..13314)
                     /locus_tag="OO544_001724"
     CDS             complement(13141..13314)
                     /locus_tag="OO544_001724"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylglucosamine transporter subunit
                     IIBC"
                     /protein_id="HCR6767342.1"
                     /translation="MGLNSAFGPSGLVALPLMTSAQGILPAMAVYAGGILVAWVCGFI
                     FTTLFGCRNVNLD"
     gene            complement(13327..14565)
                     /gene="murP"
                     /locus_tag="OO544_001725"
                     /pseudo
     CDS             complement(13327..14565)
                     /gene="murP"
                     /locus_tag="OO544_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040446.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
     gene            complement(14569..15465)
                     /gene="murQ"
                     /locus_tag="OO544_001726"
     CDS             complement(14569..15465)
                     /gene="murQ"
                     /locus_tag="OO544_001726"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HCR6767343.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGTEDSREGGINDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            15629..16486
                     /gene="murR"
                     /locus_tag="OO544_001727"
     CDS             15629..16486
                     /gene="murR"
                     /locus_tag="OO544_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HCR6767344.1"
                     /translation="MLYLTKIRNAESEFTENEQKIADFLRANVSELKSVSSRKMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVAYEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVESLKMIQR
                     SSELTQRLK"
     gene            16615..17406
                     /gene="ucpA"
                     /locus_tag="OO544_001728"
     CDS             16615..17406
                     /gene="ucpA"
                     /locus_tag="OO544_001728"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HCR6767345.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DDDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPL
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            17577..18593
                     /gene="cysP"
                     /locus_tag="OO544_001729"
     CDS             17577..18593
                     /gene="cysP"
                     /locus_tag="OO544_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HCR6767346.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWTKENGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQTRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRN"
     gene            18593..19426
                     /gene="cysT"
                     /locus_tag="OO544_001730"
     CDS             18593..19426
                     /gene="cysT"
                     /locus_tag="OO544_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HCR6767347.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            19426..20301
                     /gene="cysW"
                     /locus_tag="OO544_001731"
     CDS             19426..20301
                     /gene="cysW"
                     /locus_tag="OO544_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HCR6767348.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            20291..21388
                     /gene="cysA"
                     /locus_tag="OO544_001732"
     CDS             20291..21388
                     /gene="cysA"
                     /locus_tag="OO544_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HCR6767349.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRDRPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPEQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            21522..22433
                     /gene="cysM"
                     /locus_tag="OO544_001733"
     CDS             21522..22433
                     /gene="cysM"
                     /locus_tag="OO544_001733"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HCR6767350.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(22436..22804)
                     /locus_tag="OO544_001734"
     CDS             complement(22436..22804)
                     /locus_tag="OO544_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HCR6767351.1"
                     /translation="MKKIICLVITLLMTLPAYAKLTVHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAQ"
     gene            22909..23760
                     /gene="pdxK"
                     /locus_tag="OO544_001735"
                     /gene_synonym="thiJ"
     CDS             22909..23760
                     /gene="pdxK"
                     /locus_tag="OO544_001735"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HCR6767352.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
BASE COUNT         5414 a         6101 c         6760 g         5501 t
ORIGIN      
        1 gcttgccgcg tctgatccgg ccttcgggat tgcacatgta gggcggataa ggcgtttacg
       61 ccgcatccgc caataaataa caactaacag ggagtaaagg cgatgggaat taacgaaatc
      121 atcatgtaca tcatgatgtt ctttatgctg atagctgccg tagacaggat cctgtcgcag
      181 ttcggcggtt ctgctcgttt cctcggtaag ttcggtaaaa gtatcgaagg atcaggcggt
      241 cagttcgaag aaggctttat ggcaatgggc gctctgggtc tggcgatggt cggtatgacc
      301 gcgctggcac cggtactggc tcacgtactc gggccggtaa ttatcccggt ttacgaaatg
      361 ctcggcgcaa acccgtcaat gttcgccgga acgctgctgg cgtgcgatat gggcggcttc
      421 ttcctcgcca aagagctggc gggcggcgac gtagcagcgt ggctatactc tgggttaatt
      481 cttgggtcga tgatggggcc aacgattgtg ttttccattc cggtggcgct cggcattatc
      541 gaaccttctg accgtcgtta tctggcgctc ggcgtgctgg cgtgcattgt gaccattccg
      601 attggctgta ttgctggtgg tctggttgct atgtactccg gtgtgcagat caacggtcag
      661 ccggtggaat tcactttcgc cctgatcctg atgaacatga tcccggtgat cattgttgcg
      721 attctggtgg cgctggggct gaaattcatc ccggaaaaaa tgatcaacgg cttccagatc
      781 ttcgccaaat tcctcgttgc attgatcacc ctcggtcttg ctgccgcggt agttaagttt
      841 ctgcttggct gggaactgat ccccggtctg gaccctatct ttatggcccc tggcgataaa
      901 cccggtgaag tgatgcgcgc cattgaagtt atcggttcta tctcctgcgt tctgttaggg
      961 gcgtatccaa tggtgctgct gctgacccgc tggtttgaaa aaccgctgat gagcgtcggt
     1021 aaagtgctga atatgaacaa catcgcggca gccggcatgg tggcaacgct tgccaacaac
     1081 atcccgatgt tcggcatgat gaagcagatg gatacccgcg gcaaagtcat caactgcgcc
     1141 ttcgccgttt ccgctgcttt cgccctgggc gaccacttag gcttcgccgc tgccaacatg
     1201 aacgccatga tcttcccgat gattgtcggc aagttgatcg gcggcgtaac ggcgattggc
     1261 gtggcgatga tgctggtgcc aaaagaagac gcgaccacgg ctaaaaccga agcggaggca
     1321 caatcgtgaa cactcgccag ctattgagcg tcggtatcga tatcggcacc accaccaccc
     1381 aggtgatttt ctcccacctg gagctggtta accgtgcggc ggtgtcgcag gtgccgcgct
     1441 acgaattcat taaacgcgaa attagctggc aaagcccggt gttctttacc cctgtcgata
     1501 aacagggtgg tttaaaagaa gcggaactga aaaccttaat cctcgagcaa tatcaggctg
     1561 cgggcattgc gccggaaagc gttgattctg gtgccatcat catcaccggt gaaagcgcga
     1621 aaacccgcaa tgctcgtccg gcggtgatgg tgctctccca gtcgctgggc gattttgtcg
     1681 ttgccagcgc cgggccgcac ctcgaatccg tcattgccgg tcacggtgcc ggggcacaaa
     1741 ccctttctga acaacggctg tgtcgggtac tgaatattga catcggcggt ggcactgcga
     1801 actacgccct gttcgatgcc ggaaaaatca gcggcactgc ctgcctcaac gtcggtggtc
     1861 gcctgctgga aaccgacagc caggggcgcg tggtttacgc tcataaaccg gggcagatga
     1921 ttgtggatga gtgcttcggc gcaggcaccg acgcccgttc gctgaccggc gcgcagctgg
     1981 tgcaggttac ccggcggatg gcagaactga ttgtcgaagt gattgacgga acgctctcgc
     2041 cgctcgcgca ggcattgatg caaaccggtt tgctgcccgc aggtgttacg cccgaaatca
     2101 ttacgctttc tggcggcgtg ggcgaatgtt atcgccacca gcccgccgac ccgttctgtt
     2161 ttgccgatat tggcccgctg ctggcaacgg cgctgcatga ccatccgcgc ctgcgtgaga
     2221 tgaacgtaca gtttccggcg caaaccgtgc gcgccacggt gattggcgcg ggcgcgcata
     2281 ccctttcgct ctctggcagc acaatctggc ttgagggcgt acaactgccg ctgcgcaatt
     2341 tgccggtggc gatcccgatt gatgaaacgg atctggtgag tgcctggcaa caggcgctgc
     2401 ttcagctgga tcttgatccc aaaactgacg cgtacgtgct ggcgcttccc gcctcgctgc
     2461 ctgtgcgtta cgccgcggta ctgacggtca tcaacgcgct ggtcgatttc gtcgcgcgtt
     2521 ttccaaatcc gcatcccctg ctggtggtgg ccgggcagga ctttggtaaa gctctgggca
     2581 tgttgttgcg cccacagcta caacaactcc cgttggcagt cattgacgaa gtgattgtcc
     2641 gcgcggggga ctatatcgac attggtacgc ctctttttgg cggatcggtt gtgccggtga
     2701 cggtgaaatc actcgcattt ccttcctgag ggaacgactt atgaaactaa agaccacatt
     2761 gttcggcaat gtatatcagt ttaaggatgt aaaagaggtg ctggctaaag ccaacgaact
     2821 gcgttcgggg gatgtgctgg cgggcgttgc agcggcaagc tcacaggagc gcgtggcggc
     2881 aaagcaggtg ttgtcggaaa tgaccgtagc ggacatccgc aataatccgg tgattgccta
     2941 tgaagatgac tgcgtgacgc ggctgattca ggacgatgtt aacgaaacgg cctacaacca
     3001 gattaaaaac aggagcatca gcgaactgcg tgagtatgtg ctgagcgatg aaaccagcgt
     3061 ggacgacatt gcctttaccc gcaaagggct gacctcggaa gtggtcgcgg cggtagcgaa
     3121 gatttgctcc aacgcggacc tgatctacgg cgcgaagaaa atgccggtaa tcaaaaaggc
     3181 caataccacc atcggtattc cgggcacctt cagcgcccgt ttgcagccaa atgacacccg
     3241 tgacgacgtg caaagtatcg ctgcacaaat ctacgaaggg ctttccttcg gggtgggcga
     3301 tgcggtgatc ggcgttaacc cggtaactga cgacgtggaa aacttaagcc gcgtgttgga
     3361 caccatttac ggcgtgatcg acaaattcaa catcccaact cagggctgcg tactggcgca
     3421 cgtcaccacc cagatcgaag cgattcgtcg cggcgcgcct ggcggactga ttttccagag
     3481 tatctgtggc agcgaaaaag ggctgaaaga gtttggcgtg gaactggcga tgctcgacga
     3541 agcgcgcgca gtgggtgctg agttcaaccg tatcgccggg gaaaactgcc tctacttcga
     3601 aaccgggcaa ggttcggcgc tgtctgctgg cgctaacttc ggcgcagact aggtgacgat
     3661 ggaagcacgt aactatgggc tggcgcgtca ttacgatccg tttatcgtca acaccgtggt
     3721 cggctttatt gggccggagt atctctacaa cgaccgccag attatccgtg ctggcttaga
     3781 agatcacttt atgggcaagc tgagcggcat ctctatgggc tgtgactgct gttacaccaa
     3841 ccacgctgat gctgaccaga acctcaacga aaacctgatg atcctgctcg ccaccgcagg
     3901 ctgcaactac atcatgggga tgccgctggg cgatgacatc atgctcaact atcagaccac
     3961 cgcattccac gacactgcca ctgtgcgtca gttactcaac ctgcgcccgt caccggagtt
     4021 tgaacgctgg ctggaaagca tgggcattat ggcaaacggt cgcctgacca aacgggcggg
     4081 cgatccgtca ctgttcttct gatgacgcgg agataacaca tcatggatca aaaacagatt
     4141 gaagaaattg tacgcagcgt gatggcgtca atgggacaag cggcccccgc gccgtcagaa
     4201 gcaaagtgcg ccaccactaa ctgtgcggca ccggtgacct cggaaagctg cgcgctggat
     4261 ttaggttctg cggaagcaaa agcgtggatt ggtgttgaaa acccgcatcg cgcagacgta
     4321 ttaacagaac tgcgccgcag caccgtggcc cgcgtttgta ccggtcgtac cggtccgcgt
     4381 ccacgtacgc aggcgctgct gcgtttcctg gccgatcact ctcgttcgaa agataccgta
     4441 ctgaaagaag tgccggaaga gtgggtgaaa gcgcagggct tgctggaagt acgctccgaa
     4501 attagcgaca aaaatctcta cctgactcgc ccggatatgg gccgccgcct gtgtgcagaa
     4561 gctgttgaag cgctgaaagc acagtgtgtt gctaatccgg acgtacaggt cgttatttct
     4621 gatggcctgt caactgatgc catcaccgtg aactacgaag agatcctgcc gccgctgatg
     4681 gcgggcctga aacaggccgg gctgaaagtc ggcacgccat tctttgttcg ttatggtcgc
     4741 gtgaagattg aagatcagat cggtgagatc ctcggcgcga aagtggtgat cctgctggtg
     4801 ggggaacgtc cggggttggg tcagtcagaa agcctctcct gttacgccgt ttactcgccg
     4861 cgtatggcga ccaccgttga ggccgatcgc acctgcatct ctaacattca ccagggcggc
     4921 acgccgccgg ttgaagccgc agccgtcatt gtggatttgg cgaaacgtat gctggagcag
     4981 aaagcatccg gcatcaacat gacccgataa ggaggcatca tgccagcttt agatttgatt
     5041 cgaccgtcgg taaccgctat gcgggtgatt gcctccgtta acgctgaatt tgcgcgtgaa
     5101 ctaaaattgc cgccgcatat tcgtagcctc gggctgattt ctgctgattc cgatgacgtc
     5161 acctacattg cggctgacga agcgaccaag caggcgatgg tcgaagtggt atatggccgc
     5221 tcgctgtatg cgggagcagc acacggaccg tcaccgactg ccggtgaagt gctgattatg
     5281 cttggtgggc caaacccggc ggaagtgcgt gctggtctgg atgcgatggt tgcgcatatt
     5341 gaaaatggcg ctgctttcca gtgggctaat gacgcagaaa acaccgcatt cctggcacat
     5401 gtagtttcgc gtaccggttc ttatctttca tcaaccgccg ggatcacgct tggcgatccg
     5461 atggcgtatc tggtggcacc gccgctggaa gcgacctacg gcattgatgc agcgttgaaa
     5521 tctgccgacg tacagctggt gacctatgtc ccgccaccgt ctgaaaccaa ctactcggca
     5581 gcatttttaa ccggtagcca ggccgcgtgt aaagcagcct gtaacgcctt taccgatgca
     5641 gtgctggaaa tcgcgcgtaa tccaatccag cgtgcgtaac ggaggttgcc gatgatcaat
     5701 gcactgggat tgctggaagt ggacggcatg gtcgccgcga tagatgcagc ggatgccatg
     5761 ctcaaagcag ctaacgttcg tctgctcagt cacgaagtgc ttgaccctgg tcgcttaacg
     5821 ctggtggtgg aaggcgatct ggcggcgtgt cgtgcggcgc tggacgcagg ttgtgctgcc
     5881 gcgatgcgta ccgggcgtgt catcagccgc aaggagatcg gtcggccaga cgatgacacc
     5941 cagtggctgg ttactggctt taaccgccag ccgaagcaac ccgtaaagga acccgacgcg
     6001 ccagttatcg tcgcggaatc tgctgacgag ttgttggcgc tgttaacatc agtacgtcag
     6061 ggaatgacgg caggagaagt ggctgcccac tttggctggc cgctggaaaa agccagaaat
     6121 gcgctcgaac agctcttttc tgccgggacg ttacgtaaac gcagtagtcg ttatcgtctc
     6181 aagccccatt aacctgtcgg aggtgccggg tgtcctacaa cacccggcat taacatcatg
     6241 aaaaagaccc gtacagccaa tttgcaccat ctttatcatg aacccttacc cgaaaacctg
     6301 aagctcacgc cgaaggtcga agtggataat gttcatcaac gacagacaac ggatgtctat
     6361 gaacatgctt tgacgattac cgcctggcag cagatttacg atcagctgca tccgggcaag
     6421 tttcatggtg aatttacgga aattctgctc gatgatattc aggtttttcg tgaatacacc
     6481 ggtctggcgc tgcgtcagtc gtgcctggtc tggccgaact cgttctggtt tggcattccg
     6541 gcgacgcgcg gtgagcaggg atttatcggt tcgcaatgtc tgggaagcgc agaaatagcg
     6601 acgcgccccg gtggaaccga gtttgaatta agtacgccgg acgattatac gatcctgggc
     6661 gtagtgctgt ctgaagatgt catcacccgt caggctaact ttttgcacaa tccggatcgg
     6721 gtattacata tgctgcgtag ccagtcggcg ctggaagtga aagagcagca taaagccgcg
     6781 ctgtggggct ttgtccaaca ggcgctggcg acgttttgcg agaacccgga aaatctccat
     6841 cagcctgcag tgcgaaaagt gctgggggat aatttgctaa tggcgatggg ggcgatgctg
     6901 gaagacgcgc aaccaatggt gacggcggaa agcatcagtc atcagagtta ccgtagatta
     6961 ctttcccgcg cccgtgaata tgtgctggaa aatatgtctg agctggtgac ggtgctggac
     7021 ttgtgtaatc aactgcatgt cagtcgccgc acgctacaaa acgcgtttca cgctatttta
     7081 ggcattggcc caaacgcgtg gctgaaacgc attcgcctga acgccgtacg ccgcgaactg
     7141 ataagcccgt ggtcgcaaag cacaacggta aaagacgccg ccatgcagtg gggattctgg
     7201 catctggggc aatttgccac ggattaccag cagctgtttg ccgagaagcc gtcgttgacg
     7261 ttgcatcagc ggatgcggga atgggggtga gggagttaca cccaatccct gaccttaata
     7321 aactcactta acgccgcttc tgggctgcca tcttttggct gataatcata ctcccagcgt
     7381 accagtggcg gcattgacat caggatcgac tcggtgcgcc cgccagtttg caggccaaac
     7441 agagtgccgc gatcccagac cagattgaac tcgacataac gaccgcgacg gtagagctga
     7501 aaattgcgct cgcgctcgcc gtaggccatc gctttacgtc gctctacaat tggtaaataa
     7561 gcgtcggtgt agcctttgcc taccgcctgc ataaaggcaa aacagtggtc gaaatctggc
     7621 gtgttcagat catcaaagaa cagcccgcca ataccgcgct gttcgttgcg atgtttgagg
     7681 tagaagtaat cgtcgcacca ctttttgtaa cggggataaa cgtcttcacc aaatggcagg
     7741 cacaggtcac gggcggtgcg gtgccagtga atggcgtctt cttcaaaacc atagaaaggg
     7801 gttaaatcga agccgccgcc aaaccaccag acgggctctg cacccggttt ttcggcaata
     7861 aaaaaacgca cattcgcgtg gctggtggga acatacgggt tatgcggatg cactaccagt
     7921 gaaacgccca tcgcctcgaa actgcgcccg gcaagttccg ggcgatgagc ggtggcggaa
     7981 gcaggcatcg cctcgccgtg gacgtgagaa aagttgacgc ctgcctgttc gaaaacacca
     8041 ccattacgca acacccgact acgcccgccg ccgccagctt cgcgctgcca actatcttcg
     8101 acaaattctg cgccatcgac ggcggtcagc tgctgacaaa tcgtatcctg aaggttgagc
     8161 agaaactgtt taacctggtg tgcgtcgggt ttcataactt atcgcttttt cgaatgtgct
     8221 ttctggttgt cgaaccagtg gaaataactg atcacgcctt cagcaatcgc tgtggcgatt
     8281 ttctgacgaa acgccgccgt gcctaacagc cgctcttctt ccgggttggt gataaacgag
     8341 gtttccacca gcaccgaagg aaccgacggc gatttcaaca ccacaaacgc ggcttgttcg
     8401 gtgttgcggc tgtgcagttt atgcaccggc ttaatctttt tcagaatatg cgagccaagc
     8461 gtcaggctgt ttttaatagt atcggtttgc accagatcaa acagcacttg ctgcaatagg
     8521 tgatccttgt cagtcgcctt tttaccggca acttcatcgg cgcggttttc gcgttcagac
     8581 aggtatttcg ccattgcgct acttgcccca cggttagaga gggcaaatac cgaagcaccg
     8641 gcagctttcg ggttggtaaa gccatcggca tgaattgaca taaacagatc tgcgccatgt
     8701 ttatgggcga tttcaacgcg atcgtaaagt gggataaacg tatcgccaga acgcgttaaa
     8761 cgcgcatcaa tcccatgatt acgcaaaatg gaacggacgt ttttagcaat cgccagcacc
     8821 acatgttttt ctttcgaacc gttgcgtccg atcgctccgg tatcaattcc gccgtggcct
     8881 ggatcgagaa caacgacacg tttgccgcca gattttttgg ctttcggctt gctgtgtccg
     8941 ttgctggttt ttaaaggttc gtctttggcg atggcttgcg acattcctga caacgtcagg
     9001 gcagccaaac cggctttcag cacctggcgg cgcgaagtga gtgtttttag tggtttaaaa
     9061 gtgctcatac ggcctgagtt gtaataataa agttccagat gttatatcgt atcgcgtaat
     9121 ccgttacgac ggtttgatgt tgagaattgt tttacttttc atttcaatag ttgacaactc
     9181 cccattatgc cactttttca accgattttc gtcagatatt ggctaattcg accgttcacg
     9241 ccataatcag ccttttaacc cgcaaaaagg taacgaatac catggagata cgcgtatttc
     9301 gccaggaaga tttcgaagag gtcatcaccc tttgggagcg ttgcgacttg ctgcgtccgt
     9361 ggaacgatcc ggaaatggac atcgagcgta agatgaacca tgacgtcagt ttgtttttgg
     9421 tcgctgaggt aaacggtgaa gtggtcggaa cggtgatggg cggttatgac gggcatcgcg
     9481 ggtctgctta ttatcttggc gtgcatccag agtttcgtgg gcgtgggatt gccaatgcgt
     9541 tgcttaatcg gctggagaaa aagctgattg ctcgtggctg cccgaaaatt cagatcaatg
     9601 tgccggaaga taacgacatg gtgctcggaa tgtatgaacg cctgggatat gaacacgccg
     9661 acgtgctgag tctgggtaag cgtttgattg aagatgaaga gtactgagtt ccatcctgtc
     9721 cattatgatg cgcacggacg cctgcgttta cccttgctct tctggcttgt gctattgctt
     9781 caggcgcgaa cctgggtgct gtttgtcatt gccggtgcgt cgcgcgagca gggtactgcg
     9841 ctgctaaatc tgttttatcc cgatcacgat aatttctggc tggggctgat tccaggcatt
     9901 ccggcggtgc tggcgttttt gttgagcggt cggcgggcta catttcctcg cacttggcat
     9961 gtgctctatt ttctgttgct gttggcgcag gtggttttac tttgctggca accgtggctg
    10021 tggctgaacg gtgaatccgt tagcggtatc ggtctggcgc tggtagtggc ggatattgtg
    10081 gcgttaatct ggctgctgac caatcgacgt ttacgcgctt gttttaatga agaaaaagaa
    10141 taacggcact ttttggtgaa tttgcactcc aagcaacgtt attgaataac caaaggaagt
    10201 gacatgaaat cgctgcgttt aatgttatgc gctatgccgt tgatgctgac cggctgttcc
    10261 acgatgtcgt cagttaactg gtcggccgct aacccgtgga actggtttgg atcgtccacc
    10321 aaagtgagcg agcagggcgt gggtgaatta acggcgtcca caccactgca agaacaagcc
    10381 attgctgatg cgcttgatgg cgattatcgc ctgcgcagcg gaatgaaaac cgcgaacggc
    10441 aacgtggtgc gcttttttga agtgatgaaa ggcgacaacg tggcgatggt gattaacggc
    10501 gatcaaggca cgatcagtcg cattgacgtg ctggatagcg atattcctgc tgacaccggt
    10561 gttaaaatcg gtacaccgtt tagcgacctt tacagcaaag catttggcaa ttgccaaaaa
    10621 gctgatggtg atgataatcg tgccgtcgaa tgtaaagccg aaggcagtca acatattagc
    10681 taccagttca gcggggaatg gcgtggtcct gaagggttaa tgccttcgga cgataccctg
    10741 aaaaactgga aagtcagtaa aattatctgg cggcgttaat ttgcgtctga acaaaccgcc
    10801 gccgcgaaaa aacacgtaaa atagcgctca acaatgccac ggattgcgtg gcattcttat
    10861 tttcaggagg aacaatgtct caggttcaga gtggcatttt gccagaacat tgccgcgcgg
    10921 cgatttggat cgaagccaac gtgaaagggg aagttgacgc cctgcgtgcg gccagtaaaa
    10981 catttgccga caaactggca acttttgaag cgaaattccc ggacgcgcat cttggtgcgg
    11041 tggttgcctt tggtaacaac acctggcgcg ctctgagcgg cggcgttggg gcagaagagc
    11101 tgaaagattt tccgggctac ggtaaaggcc ttgcgccgac cacccagttc gatgtgttga
    11161 tccacattct ttctctgcgt cacgacgtaa acttctctgt cgcccaggcg gcgatggaag
    11221 cctttggtga ctgcattgaa gtgaaagaag agatccacgg cttccgttgg gtggaagagc
    11281 gtgacctgag cggctttgtt gacggtacag aaaacccggc gggtgaagag acgcgtcgcg
    11341 aagtggcggt tatcaaagac ggcgtggatg ccggcggcag ctatgtgttt gtgcagcgtt
    11401 gggagcacaa cctgaagcag ctcaaccgga tgagcgttca cgatcaggag atgatgatcg
    11461 ggcgcaccaa agaggccaac gaagagattg acggcgacga acgtccggaa acctctcacc
    11521 tcacccgcgt tgatctgaaa gaagatggca aagggctgaa gattgttcgc cagagcctgc
    11581 cgtacggcac cgccagtggc actcacggtc tgtacttctg cgcctactgc gcgcgtctgc
    11641 ataacattga gcagcaactg ctgagcatgt ttggcgatac cgatggtaag cgtgatgcga
    11701 tgttgcgttt caccaaaccg gtaaccggcg gctattactt cgcgccgtcg ctggacaagt
    11761 taatggcgtt gtaagcgttt ctgattcgat gattaaaggc cagccagtaa caaaactgac
    11821 tggccttttt attacttttg cttcaacgcc gcatacacct gatcaactac ccaaccataa
    11881 gttgcaatcg gtaactgacc gctttcgaac atattgggat ttttttgtgg atcggcaact
    11941 ggcgaatggg gcttgttgct taacatcaca atcgccatat gattgaccgg atcgataacg
    12001 gttactgtcc cggtccagcc agtgtgaccg taggtttgcg ggcttgccag cgtgccaaac
    12061 gtcggcgtca tggtggcatt accattcacg cgccagccga ggccaaaagt ggcatcttcc
    12121 ttagagctgg tggtgaacat cttcaccgtt tccgcactga acagctgcac atcgccatag
    12181 cccccgccgt tcagcatcgt ttgcattaac accgcaatat cgccggtatt ggaaaacaaa
    12241 cctgcgtgcc cggaaacgcc gcccatcgaa taaaaggctt tttcatcgtg cacctgaccc
    12301 cagagagtgg aggtgcggat attcgggaaa tggatcacgc catcgcgggt attgccgttc
    12361 aattccgtgg cggcaatttg ttgcggttta aagcctttca gcagcgggtt aaataccgta
    12421 tgtgtcaggc cgagcgggcg ataaatcgac tcttcaacat agcggtcaag cggctgaccg
    12481 gtaaccgact cgacgataaa tccaagcagc atataatcga catcgctgta gatatgtttg
    12541 ctgccgggct gatattccag cggcgtgcgc ttgatcatct ccagcgtctg gcctttatcc
    12601 tgggaatata acgcgcccgc gaccgcttta ttcgggtatt gcggatctgc cgggaaaccg
    12661 ccgctgtgat gcagcaggtc agaaatccgc agggtgtttt tgcctttaat ggtgtcattt
    12721 ggactatcgg caaaccccgg aatatatttc gcaatccgat cgtcaggatg cagtttgcct
    12781 tcgctcatca gcttttgcag ggcgaagttc gtggcgtaca ttttggtgtt tgaggccagg
    12841 tcatacagcg tcccggtggt ggctttgaca ggctgttcca tcaacacgct gccatcgtac
    12901 tttttcgccg ctccccaggc tttgcgatac acaatctgat tatctttaat gatcagcagg
    12961 tttacgccgg gataaccgac atcaatttgc tggctaatcc agcgatccat ctggttaagc
    13021 cgttcgacgt taaaccctgc tttctctggc gagctttctg tcagaacagg gtattttgcg
    13081 gcgctgacgc tacaactaac agccagcaga gaaagataga gcattgtccg tttcatgata
    13141 tcagtccaga ttgacgttac ggcagccaaa gagcgtggtg aaaataaacc cgcaaaccca
    13201 tgccaccaga atcccgccag cataaaccgc catggccgga aggatgcctt gtgcggaagt
    13261 catcagcggc agcgctacca gaccagacgg cccaaaggcg ctgtttaagc ccatcggcag
    13321 accccatcag gctatcaggc cgataaacaa accgcccgcc gcgccgccta aacaggcggt
    13381 aacaaacggc ttcatgcggg gcagggtgac accgtaaatc agcggttcac caacgcccag
    13441 caggccggga ataatcgccc cgcgtacctg actgcgtagc gcactgtgcg gttgcgcccg
    13501 ccagtagagt gccagcgccg cgcccacctg gcccgcgcct gccattgaaa ggatgggaaa
    13561 taagctgttg aatccctagc tgtccattaa cgcgaggtaa acaggaataa agccctgatg
    13621 cacgccaaat accacggcga tcaggaacag cccggctaaa accgcacaac cgaacggatt
    13681 actgttcagg tgcataaaca gccatgacat accttcgaat aaccagccac ccagcggcat
    13741 aatgatcagg tacgcaagcg tagcggtgat cagcagggtg attaacgagg tcagcagcat
    13801 gtcgagatcg tccgacataa agcggcgcac catgccttca atgcgggcgc aggcccacgc
    13861 ggcaatcagc acgccgataa tattgccgcg cggatcgatg ggcagaccaa agaaatcgtg
    13921 aaaaccggcg tagtcaccgg tggttgcagc ggggttataa ccgagcaaaa agagtgcggc
    13981 aataattgcg ccattgacgc ccgtgccgcc aaatgcctgg gcggcgttat agcccaccag
    14041 aatcaccagg aaagtgaaca aacctttgct gaacaccttc ataaaattca gcgcgtcggg
    14101 tagcgtgccc tgagcatctg ccggaacgtg catcaccgtg gcaattaacg tcgctatccc
    14161 cagcagcaga ccggcggcaa taaaaccggg gatcagcggc gtgaagatgg tggcgaattt
    14221 ggcgagaaat tgttgcacgc cggaggtttg tttggctttt aactgacgtt tgttctgggc
    14281 ggcgatttct gcggcatcct gtaccggtgc ctcgcccagc agctcgctca tcgcttttgc
    14341 ggcacgatgc gctttaccag gcccaaaaac gacctgcacc tggtcgctgg tcaaaatgac
    14401 gcccttcacg ccttccagcg ttttgatatt gggatcaacc agtgaactgt catgtacacc
    14461 cagacgcagg cgcgtcatac agttaccaca actggcgata tttcccagtc cgccgacacg
    14521 ggtaagaatg gtgttcagaa gttcactgct gatctctttg gccatgggtt attccttgtc
    14581 taaaacctga cgaataaagc cgccgtgttg atccaggcgt tttttagctt ctgcggcatc
    14641 gagatttttc agcaccatca caatggccgt tttacagttg cgctcgcagg caattaacgc
    14701 cgcttccgct tgttcggcat tacatccggt ggcgttttta acgatattga cctggcgcac
    14761 atgcagtttt tcgttggtgg cgaccacatc gaccatcagg ttgccgaaca ctttgccgga
    14821 tttaatcatc agcccggtgg aaagcatatt gagcaccagt ttctgcgctg tgcctgcttt
    14881 cattcgcgaa gaaccagtga ccacttcggc acctacaatc ggtgtaatgg caaactcggc
    14941 ggtggtggaa acggcactcc ccggattaca ggaaattccc actgtgcggc aaccgagctg
    15001 gcgggcgtat tccagtccgg caatcacata cggcgtacga ccgctggcgg caataccgac
    15061 caccacatcc tgtgccgtta aatttatatt tttcagatcg ttaatgccgc cttctcggct
    15121 atcttccgtg ccttccaccg cgtgctgaat ggcatattcg ccgccagcaa tcaaaccaac
    15181 caccagaccc ggtttcacgc cgtaggtggg cggacattcg ctggcatcca gaatccccag
    15241 acgaccggat gttcccgcac cgagataaat cagacgaccg ccgccgctta cctgggcgtg
    15301 gataacatcg atcgccgcgg cgatatccgg caggacgcgc tcaacggcaa gcggtacggt
    15361 tttatcttca tcgttgataa tccggcacat ttccagcgtc gatacgcggt caatttcagc
    15421 cgaggcggtg ttcgagcctt cagtaatcat cttttcaagt tgcatcggtg gtgaccttac
    15481 tatttacgat caaataattg cagatgtatg gaatcatata tcccgaattg acagacttaa
    15541 aggaatcatt gagtctacac ttgcagagtg tgaagagcgt cataagaact taagcggaat
    15601 ttttgctgcc agcccgggag agtggaagat gttgtacctg acgaaaatac gcaatgcgga
    15661 aagtgaattt accgagaacg agcaaaaaat tgctgacttt ctgcgggcca acgtgagtga
    15721 gctgaaatcc gtctcttcgc gcaagatggc gaaacagctc ggcatcagtc agtcgagcat
    15781 tgtgaaattt gcccagaagc tcggcgcgca aggatttact gaattgcgta tggcgttaat
    15841 tggcgaatac agtgccagcc gggagaaaac gaatgccacg gcgctgcacc tgcacagttc
    15901 gattaccagt gatgattcgc tggaagtgat tgcccgcaaa ctgaatcgcg aaaaagagct
    15961 ggcgctggag caaacctgtg cgttgttcga ctacgctcgg ctgcaaaaaa tcattgaggt
    16021 catcagtaaa gcaccgttta tccagataac gggcctgggc gggtcggcgc tggtggggcg
    16081 cgatctgtcg ttcaaactga tgaaaattgg ctatcgcgtg gcctatgaag ccgacacaca
    16141 cgtacaagcc accgtttccc aggcgttgaa aaaaggcgat gtacaaatcg ctatctctta
    16201 cagcggcagt aaaaaagaga ttgtcttgtg cgcagaagcg gcacgaaagc agggggcaac
    16261 ggtgattgcc attacttctc tggctgactc tccgctgcgg cggctggcgc atttcacgct
    16321 cgataccgtt tccggtgaaa ccgaatggcg tagctcgtcg atgtctaccc gcacggcgca
    16381 aaattctgtt accgatttac tgtttgttgg tcttgtgcaa ctcaatgatg tggagtcatt
    16441 gaaaatgatt cagcgcagca gtgagctaac acaacgcctg aaatgatgca taaagcagcg
    16501 actggattga gattttcctg aattagtgag ctgatccgca gcaatatttt gtttatcctg
    16561 tattttcaga gggaatggag tgtaacgctc tgtattaaca aggagagcat taaaatgggt
    16621 aaactcacgg gcaagacagc actgattacg ggcgcattgc agggaattgg cgaaggaatt
    16681 gccagaactt ttgcgcgtca tggcgcaaac ctaatcttgc tggatatctc ccctgagatc
    16741 gaaaagctgg cggacgaact gtgtggtcgt ggtcatcgct gtacggcggt tgtcgccgat
    16801 gtgcgtgacc cggcgtcggt agccgcagct atcaaacgcg cgaaggaaaa agaagggcgc
    16861 attgatatcc tggtgaataa cgcaggcgtt tgtcgtctgg gcagtttcct cgatatgagc
    16921 gatgacgatc gcgatttcca tattgacatc aatattaaag gcgtatggaa cgtcacgaag
    16981 gcggtgctgc cggagatgat tgcccgcaaa gatggtcgca ttgtgatgat gtcttcagtc
    17041 actggtgata tggtggccga tcctggcgaa acggcgtacg ccttaacgaa agcggcgatt
    17101 gttggcctga caaaatcgct ggcggtggag tacgcgcagt ctggtattcg cgttaacgcc
    17161 atttgcccgg gatacgtgcg cacaccaatg gcggaaagca ttgcccgcca gtcgaacccg
    17221 gaagatccag agtcggtgct gactgaaatg gcgaaagcaa tcccgctgcg tcgcctcgcc
    17281 gatccgctgg aagtcggcga actggcggcc ttcctcgcat cggatgaatc cagctattta
    17341 accggtacac agaatgtgat tgatggcggc agcacactgc cggagacggt tagcgtcggt
    17401 atctgattca cctctgtttc ctccctgcat ttgtggggag gatttcgttt taaacccggt
    17461 tcttattccc ttttcaactt ccaaatcacc aaacggtata taaaaccgtt actcctttca
    17521 cgtccgttat aaatatgatg gctattagaa agtcattaaa tttataaggg tgcgcaatgg
    17581 ccgttaactt actgaaaaag aactcactcg cgctggtcgc ttctctgctg ctggcgggcc
    17641 atgtacaggc aacggaactg ctgaacagtt cttatgacgt ctcccgcgag ctgtttgccg
    17701 ccctgaaccc gccgtttgag caacaatgga ccaaagaaaa cggcggcgac aaactgacaa
    17761 taaaacaatc tcatgccggg tcatcaaaac aggcgctggc aattttgcag ggcttaaaag
    17821 cagacgttgt cacttataac caggtgactg acgtacaaat cctgcatgac aaaggcaagc
    17881 tgatcccggc ggactggcaa acgcgcctgc caaataatag ctcgccattc tactccacca
    17941 tgggcttcct ggtgcgtaag ggcaacccga agaatatcca cgactggaac gacctggtgc
    18001 gctccgatgt gaagctgatt ttcccgaacc cgaaaacgtc gggtaacgcg cgttacacct
    18061 atctggcggc atggggcgca gcggataaag ctgacggtgg cgataaagcc aaaaccgaac
    18121 agtttatgac ccagttcctg aaaaacgttg aagtgttcga tactggcggt cgtggcgcga
    18181 ccaccacttt tgccgagcgc ggcctgggcg atgtgctgat cagcttcgag tcggaagtga
    18241 acaacatccg taaacagtat gaagcgcagg gttttgaagt ggtgattccg aaaaccaaca
    18301 ttctggcgga attcccggtg gcgtgggttg ataaaaacgt gcaggccaac ggtacggaaa
    18361 aagccgccaa agcctacctt aactggctct acagcccgca ggcgcaaacc atcatcaccg
    18421 actattacta ccgcgtgaat aacccggaag tcatggataa actgaaagac aaattcccgc
    18481 agaccgagct gttccgcgtg gaagacaaat ttggctcctg gccggaagtg atgaaaaccc
    18541 acttcaccag cggcggcgag ttagacaagc tgttagcggc ggggcgtaat taatgtttgc
    18601 agtttcatca agacgcgtgc tgccgggctt taccttaagc ctcggcacca gtctgctgtt
    18661 tgtgtgcctg attttgctgc tgccgctctc cgcgctggtg atgcagctgg ctcagatgag
    18721 ctgggcgcag tactgggagg tgatcaccaa cccgcaggta gtagcggcct acaaagtaac
    18781 gctgctgtca gcgtttgtgg catcgatttt taacggcgtt tttggcctgc tgatggcgtg
    18841 gatcttaacc cgctatcgct ttccgggccg cacgctgctt gatgcgctga tggatttacc
    18901 ctttgcgctg ccaacggcag tcgccggttt aacgctagcc tcgctctttt ctgtaaacgg
    18961 tttttacggt gaatggctgg cgaagtttga tatcaaagtc acctatacct ggctggggat
    19021 tgcggtggcg atggccttta ccagcattcc gtttgtggta cgtaccgtgc agccggtgct
    19081 ggaagagtta ggcccggaat atgaagaagc ggcagaaacg cttggcgcaa cgcgctggca
    19141 gagtttctgc aaagtggtgc tgccggaact ttctccggcg ctggtggcgg gcgtggcgct
    19201 gtcgtttacc cgtagtcttg gtgaatttgg cgcggtgatt tttatcgccg gaaatatcgc
    19261 gtggaagacg gaagtgacgt cgctgatgat ttttgtgcgc ttacaggagt ttgattaccc
    19321 ggcagcgagc gcgattgctt cggtgatcct cgcggcatct ctgctgctgc tgttctcaat
    19381 taacactctg caaagtcgct ttggtcggcg tgtggtaggt cattaatggc ggaagttacc
    19441 caattgaagc gttatgacgc gcgcccgatt aactggggca aatggtttct gattggcatc
    19501 gggatgctgg tatcggcgtt catcctgctg gtgccgatga tttatatctt cgtacaggcg
    19561 ttcagcaaag ggctgatgcc ggttttacag aatctggccg atccggacat gctgcacgcc
    19621 atctggctga cggtgatgat cgcgctgatt gccgtaccgg taaacctggt gttcggcatt
    19681 ctgctggcct ggctggtgac gcgctttaac ttccctggac gccagttact gctgacgcta
    19741 ctggacattc cgtttgccgt atcgccggtg gttgccggtc tggtgtatct gctgttctac
    19801 ggctctaacg gcccgctggg cggctggctg gatgaacata atctgcaaat tatgttctcc
    19861 tggcccggaa tggtgctggt caccatcttt gtgacgtgtc cgtttgtggt gcgcgagctg
    19921 gtgccggtga tgttaagcca gggcagccag gaagacgaag cggcgatttt gcttggcgcg
    19981 tctggctggc agatgtttcg ccgcgtcaca ttgccgaaca ttcgctgggc gctgctttat
    20041 ggcgtggtgt tgaccaacgc ccgcgcaatt ggcgagtttg gcgcggtgtc ggtggtttcc
    20101 ggctcgattc gcggcgaaac cctgtcgctg ccgttacaga ttgaattgct ggagcaggac
    20161 tacaacaccg tcggctcctt taccgctgcg gcactgttaa cgctgatggc gattatcacc
    20221 ctgtttttaa aaagtatgtt gcagtggcgc ctggagaatc aggaaaaacg cgcacagcag
    20281 gaggaacatc atgagcattg agattgccaa tattaagaag tcgtttggtc gcacccaggt
    20341 gctgaacgat atctcactgg atattccttc aggtcagatg gtcgcgttgc tggggccgtc
    20401 cggttccggg aaaaccacgc tgctgcgcat tatcgccggg ctggagcatc aaaccagcgg
    20461 gcatattcgc ttccacggca ccgacgtcag ccgcctgcac gcgcgcgatc gcaaggtcgg
    20521 tttcgtgttc cagcattacg cgctgttccg ccatatgacg gtattcgaca atatcgcttt
    20581 tggcctgacg gtgctgccgc gtcgcgatcg cccgaatgcc gcagccatca aagcgaaagt
    20641 gacaaaattg ctggagatgg tgcagcttgc tcatctggcg gatcgttatc cggcacagct
    20701 ttccggcggc cagaaacagc gcgtggcgct ggcgcgcgcg ctggctgtag aaccgcaaat
    20761 tctgctgctt gatgaaccgt ttggcgcgct ggatgcgcag gtgcgtaaag agctgcgtcg
    20821 ctggctgcgt caactgcatg aagagctgaa attcaccagc gtgttcgtga cccacgacca
    20881 ggaagaagcg acggaagtag ctgatcgtgt agtggtgatg agccagggca atattgaaca
    20941 ggctgatgcg ccggagcagg tatggcgcga accagcgacc cgttttgtgc tcgaatttat
    21001 gggcgaagtg aaccgcctgc agggaaccat tcgcggcggg cagttccatg ttggcgcaca
    21061 tcgctggccg ctgggctaca cacctgctta tcaggggccg gtggatctct tcctgcgccc
    21121 gtgggaagtg gatatcagcc gccgtaccag tctcgattcg ccgctgccgg tacaggtact
    21181 ggaagccagc ccgaaaggtc actacaccca attagtggtg cagccgctgg ggtggtacaa
    21241 cgaaccgctg acggtcgtga tgcatggcga cgatgccccg cagcgtggcg agcgtttatt
    21301 cgttggtctg caacatgcgc ggctgtataa cggcgacgag cgtatcgaaa cccgcgatga
    21361 ggaacttgct ctcgcacaaa gcgcctgata ggttgagtga atgttaaacg cccggaggcg
    21421 cttcccgcga tccgggcttt ttaatggcaa ggtttgtaac ctgtagacct gataagacgc
    21481 gcaagcgtcg catcaggcaa caccacgtat ggatagagat cgtgagtaca ttagaacaaa
    21541 caataggcaa tacgcctctg gtgaagttgc agcgaatggg gccggataac ggcagtgaag
    21601 tgtggttaaa actggaaggc aataacccgg caggttcggt gaaagatcgt gcggcacttt
    21661 cgatgatcgt cgaggcggaa aagcgcgggg aaattaaacc gggtgatgtc ttaatcgaag
    21721 ccaccagtgg taacaccggc attgcgctgg caatgattgc cgcgctgaaa ggctatcgca
    21781 tgaaattgct gatgcccgac aacatgagcc aggaacgccg tgcggcgatg cgtgcttatg
    21841 gtgcggaact gattcttgtc accaaagagc agggcatgga aggtgcgcgc gatctggcgc
    21901 tggagatggc gaatcgtggc gaaggaaagc tgctcgatca gttcaataat cccgataacc
    21961 cttacgccca ttacaccact accgggccgg aaatctggca gcaaaccggc gggcgcatta
    22021 cccattttgt ctccagcatg ggaacaaccg gcactatcac cggcgtttca cgctttatgc
    22081 gcgaacaatc caaaccagta acgattgttg gcctgcagcc ggaagaaggc agcagtattc
    22141 ccggcattcg ccgctggcct gcggaatatc tgccggggat tttcaacgct tctctggtgg
    22201 atgaggtgct ggatattcat cagcgcgatg cagaaaacac catgcgcgaa ctggcagtgc
    22261 gggaaggaat attctgtggc gtcagctccg gcggcgcggt tgccggagca ctgcgggtgg
    22321 caaaagctaa ccctggtgcg gtggtggtag cgatcatctg cgatcgtggc gatcgctacc
    22381 tttccaccgg ggtgtttggg gaagagcatt ttagtcaggg ggcggggatt taagattatt
    22441 gcgcaggcac cgttttatcc gtctgcgcaa ttaaggcatg taaaaaaggc tgtgcgttct
    22501 catcgctctt accggggatc ttcacaatat acggcttccc ggtaatcgat gacgacgaag
    22561 caaccttatc aataaactgc tcggcagtgt caatgcggtt acgggtattg ccgagcttca
    22621 gacgcagatg agaaaccgct tcatcgcagg tatgttcatc accgttgcgc acaaatatca
    22681 aatccttttt ctgtgctaat ccctccagca tggcgttgat gcgggcttct tcatgaacag
    22741 ttaacttcgc gtaagcgggg agtgtcatta gtagtgtaat gaccagacaa atgattttct
    22801 tcattgaatt accttatcct tgttttgtga tagccgattt gactgccaga atcaggtaaa
    22861 gtttcggttg gaatacgatg aggatcatcg gcaaggagaa aaaattaaat gagtagtttg
    22921 ttgttgttta acgataagag tagggcgctg caggcggata tcgtcgccgt gcagtcgcag
    22981 gtagtgtacg gcagcgtagg caacagcatt gcggtgcctg ctataaaaca gaacggcttg
    23041 aatgtctttg ccgtgccgac ggtattgctg agcaatacgc cgcattatga cactttctac
    23101 ggtggtgcga ttccggacga atggtttagc ggctatttgc gtgcgcttca ggagcgtgat
    23161 gcgctgcgcc aacttcgtgc tgtaaccacg ggctatatgg gaacggcatc gcaaatcaaa
    23221 atccttgccg agtggctgac tgcgctacgc aaagaccatc ctgacctatt gatcatggtc
    23281 gatccggtga ttggcgatat tgatagcgga atttatgtca aacctgacct tcccgaggcg
    23341 tatcgacaat atttactgcc gctggcgcaa ggaattaccc ccaatatctt tgagctggaa
    23401 atcctgaccg gtaaaaattg ccgcgatctc gacagtgcca ttgctgccgc aaaaagtctg
    23461 ctttcagaca cattaaaatg ggtggtgatt accagcgcct ccggtaatga agaaaatcag
    23521 gagatgcagg tggtggtggt cagtgccgac agcgtgaatg tcatttccca ttcacgggta
    23581 aaaaccgacc tgaaagggac tggcgacctg ttttgtgctc agcttatcag tggtttgctg
    23641 aaagggaagg cgttaaacga tgcggtgcac cgtgcggggt tgcgcgtact ggaagtgatg
    23701 cgctataccc agcagcatga gagcgatgaa ttgattttgc cgccgctggc ggaagcataa
    23761 aaaatggcgc cgatgg
//
LOCUS       DAJTZL010000050        23561 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.050, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000050 DAJTZL010000000
VERSION     DAJTZL010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23561
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.050"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..468
                     /locus_tag="OO544_001736"
     CDS             <1..468
                     /locus_tag="OO544_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6767353.1"
                     /translation="SGTSVTGSTVKMGEYSAEQIRNGATPVPFDISLQNCVRVTNIET
                     KLVSTKVGTENGQLLGNTLTGNDAAKGVGVLIEGLATSKNPLMTLKPNDSNSVYKDYD
                     PRGKDDTTGGVYPDQDTGITYPLHFQATLQQDGTIPIEAGEFKATSTFQVTYP"
     gene            581..1375
                     /gene="panB"
                     /locus_tag="OO544_001737"
     CDS             581..1375
                     /gene="panB"
                     /locus_tag="OO544_001737"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HCR6767354.1"
                     /translation="MKPTTIASLQKCKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTASVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAG
                     DQLLSDALALEAAGAQLLVLECVPIELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            1387..2238
                     /gene="panC"
                     /locus_tag="OO544_001738"
     CDS             1387..2238
                     /gene="panC"
                     /locus_tag="OO544_001738"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HCR6767355.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIIAIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKIVELA"
     gene            complement(2530..3426)
                     /gene="rpnC"
                     /locus_tag="OO544_001739"
     CDS             complement(2530..3426)
                     /gene="rpnC"
                     /locus_tag="OO544_001739"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HCR6767356.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMVFRMMRYSIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDIFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGESMMTLAQWFEEKGIEKGIQQGRQEERQEFAQRFLSKG
                     MSREDVAEMTNLSLAEIDRLIN"
     gene            3700..4080
                     /gene="panD"
                     /locus_tag="OO544_001740"
     CDS             3700..4080
                     /gene="panD"
                     /locus_tag="OO544_001740"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HCR6767357.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(4084..5313)
                     /locus_tag="OO544_001741"
     CDS             complement(4084..5313)
                     /locus_tag="OO544_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCR6767358.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLVTWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQVRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFVVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLVSNQPQG"
     gene            complement(5377..5817)
                     /locus_tag="OO544_001742"
     CDS             complement(5377..5817)
                     /locus_tag="OO544_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR6767359.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRERIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(5922..6692)
                     /locus_tag="OO544_001743"
     CDS             complement(5922..6692)
                     /locus_tag="OO544_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6767360.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(6689..7615)
                     /locus_tag="OO544_001744"
     CDS             complement(6689..7615)
                     /locus_tag="OO544_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6767361.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            7724..8386
                     /gene="can"
                     /locus_tag="OO544_001745"
     CDS             7724..8386
                     /gene="can"
                     /locus_tag="OO544_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HCR6767362.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(8427..8963)
                     /gene="hpt"
                     /locus_tag="OO544_001746"
     CDS             complement(8427..8963)
                     /gene="hpt"
                     /locus_tag="OO544_001746"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HCR6767363.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILRDE"
     gene            9169..11559
                     /gene="gcd"
                     /locus_tag="OO544_001747"
     CDS             9169..11559
                     /gene="gcd"
                     /locus_tag="OO544_001747"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HCR6767364.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWCSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGSILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHNLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VSAPEKPVPQGAAKGDYVTPTQPFSELSFRPKKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKINEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(11606..13156)
                     /gene="cueO"
                     /locus_tag="OO544_001748"
     CDS             complement(11606..13156)
                     /gene="cueO"
                     /locus_tag="OO544_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HCR6767365.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLAEETTLHW
                     HGLEVPGDVDGGPQGIIPPGGKRTVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFNADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGM
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHNAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            13322..13669
                     /locus_tag="OO544_001749"
     CDS             13322..13669
                     /locus_tag="OO544_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HCR6767366.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            13775..14641
                     /gene="speE"
                     /locus_tag="OO544_001750"
     CDS             13775..14641
                     /gene="speE"
                     /locus_tag="OO544_001750"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HCR6767367.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGIVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSYRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            14657..15451
                     /gene="speD"
                     /locus_tag="OO544_001751"
     CDS             14657..15451
                     /gene="speD"
                     /locus_tag="OO544_001751"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HCR6767368.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(15489..15851)
                     /locus_tag="OO544_001752"
     CDS             complement(15489..15851)
                     /locus_tag="OO544_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HCR6767369.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(16026..18623)
                     /gene="acnB"
                     /locus_tag="OO544_001753"
     CDS             complement(16026..18623)
                     /gene="acnB"
                     /locus_tag="OO544_001753"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HCR6767370.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            18978..20735
                     /locus_tag="OO544_001754"
     CDS             18978..20735
                     /locus_tag="OO544_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HCR6767371.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASAGLYIKSRTVEAPVETQSTQ
                     QTAPDITAVTLPATVSAPPVTPAVVKSAFSTAQIDQWVAPVALYPDSLLSQVLMASTY
                     PANVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKAVPVKQTVTAPVIPSN
                     TVLTANPVITEPATTVISIEPANPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FVDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGGHRDGNGWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQAAASQFQQRTHAAPVITRDTQRQAAAQRFNEAEHYGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVREKMQTNPQNQQRREAARERIQSATPEQRQ
                     VFKEKVQQRPLNQQQRDNARQRIQSASPEQRQVFREKVQESRPQRLNDSNHTVRLNNE
                     QRSAVRERLSERGARRQER"
     gene            complement(20806..22230)
                     /gene="lpdA"
                     /locus_tag="OO544_001755"
     CDS             complement(20806..22230)
                     /gene="lpdA"
                     /locus_tag="OO544_001755"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HCR6767372.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(22555..>23561)
                     /gene="aceF"
                     /locus_tag="OO544_001756"
     CDS             complement(22555..>23561)
                     /gene="aceF"
                     /locus_tag="OO544_001756"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HCR6767373.1"
                     /translation="PAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIRRLAREF
                     GVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKVDFSKFG
                     EIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAKRKLDVK
                     LTPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVVPVFKDV
                     NKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVNAPEVAI
                     LGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSDIRRLVM
                     "
BASE COUNT         5793 a         6409 c         5761 g         5598 t
ORIGIN      
        1 agtggaactt ctgtgactgg ttctacggta aaaatgggtg aatatagtgc agaacaaatc
       61 agaaacggtg ccacaccggt tccttttgat atttcacttc aaaactgcgt tcgtgtgact
      121 aatattgaga caaaattagt ttcaacaaag gttggtactg aaaacgggca actccttggt
      181 aatactctca caggtaatga cgcagcaaaa ggagtcggtg tactcataga aggtttagca
      241 actagtaaaa atcctctaat gacattgaaa cctaatgatt caaattctgt ttataaagat
      301 tacgacccga gaggcaaaga cgatacaaca ggaggagttt acccggatca agatacaggt
      361 ataacatacc ctctccattt ccaggccacg ctacaacagg atggaactat accaatagaa
      421 gctggtgagt ttaaagccac cagtactttc caggtaacct acccttaata agtccaccgc
      481 accgccatca tctggcggtg cggtaattga taaatcattg cccccaccaa tgtgactgat
      541 aaaatgccag gcaacacgac atcatttatc aggacacgtt atgaaaccga ccaccattgc
      601 ttcacttcag aaatgtaagc aggataaaaa gcgtttcgcg accatcaccg cttacgacta
      661 cagcttcgcg aaactatttg ctgatgaagg acttaacgtc atgctggtag gcgactcgct
      721 gggcatgacg gttcagggtc acgactccac cctgccggtg accgttgccg atatcgccta
      781 ccacactgcc tccgtacgtc gtggtgcgcc aaactgcctg ctgttggctg acctgccgtt
      841 tatggcgtat gccacgccgg aacaagcctt tgaaaacgcc gcaacggtta tgcgtgccgg
      901 tgccaatatg gtcaaaattg agggcggtga gtggctggtc gaaaccgtaa aaatgctgac
      961 cgaacgtgcc gttcctgtat gtggtcactt aggtttaacc ccacagtcag tgaatatttt
     1021 cggtggctac aaagttcagg ggcgcggcga tgaagcgggc gatcaactgc tcagcgatgc
     1081 attagcctta gaagccgctg gggcacagct gctggtgctg gaatgcgtgc cgattgaact
     1141 ggcaaaacgt attaccgaag cactggcgat cccggttatt ggcattggcg caggcaacgt
     1201 cactgacggg cagatcctcg tgatgcacga cgccttcggc attaccggcg gtcacattcc
     1261 taaattcgct aaaaatttcc tcgccgaaac gggcgacatc cgcgcggctg tgcggcagta
     1321 tatggctgaa gtggagtccg gcgtttatcc gggcgaagaa cacagtttcc attaaggagt
     1381 cacgttgtgt taattatcga aaccctgccg ctgctgcgtc agcaaattcg ccgcctgcgt
     1441 atggaaggca agcgcgtggc gctggtgcct actatgggta atctgcacga tggccatatg
     1501 aagctggtcg acgaagccaa agcccgcgcc gatgtggtcg tcgtcagtat tttcgttaac
     1561 ccgatgcagt tcgaccgccc ggaagatctg gcacgttatc cacggacctt gcaggaggac
     1621 tgcgagaagc taaacaaacg taaagtggat ttagttttcg ccccttcggt aaaagagatc
     1681 tacccgaacg gtactgaaac ccatacttac gttgacgttc ccgggctttc gaccatgctg
     1741 gaaggcgcga gccgtccggg gcattttcgc ggcgtttcga ctatcgtcag caagctgttt
     1801 aacctggtcc agccggacat cgcctgcttc ggtgaaaagg actttcagca actggcgcta
     1861 atccgcaaaa tggttgccga tatgggcttc gatattgaaa ttgtcggcgt cccgattatg
     1921 cgcgccaaag atggccttgc gctcagttcc cgtaatggtt atctgacggc ggaacaacgc
     1981 aaaattgcgc ccggtctgta caaagtttta agttcaattg ccgacaaatt gcaggctggc
     2041 gagcgggatc ttgatgaaat tattgccatt gcagggcaag aactgaatga aaaaggcttc
     2101 cgcgccgatg atattcagat tcgcgatgcc gacacgttgc tggaagtctc tgaaaccagc
     2161 aaacgagctg taattctggt agccgcctgg cttggcgatg cccgcctgat cgacaacaaa
     2221 atcgtcgaac tggcgtaatg cttttactct aaataattca agttgcagga aggcggcaag
     2281 cgagtgacaa attcgtcagg aacgaatttg cccagccgca ggctggcctt cggtgagaga
     2341 catggatgtc tctcattaat ccccaggagc ttacataagt aagtgactgg ggttagcgaa
     2401 cgcagacgca gcacatgcaa tttaaagtat gacgagtata actggcgcta cggctgatgg
     2461 cgccaattat taatttgccc cacgtcatgg cgatggcgtc tgtaagcaat tattcataac
     2521 gcagacaact tagttaatca gcctatcaat ctcagcaaga gataaatttg tcatctctgc
     2581 aacgtcttcc cgagacatcc ctttactcag aaaacgctgg gcgaattctt gcctttcctc
     2641 ctgtcttccc tgctgaatcc ccttctcaat ccctttctct tcaaaccact gcgccagcgt
     2701 catcatagac tcccctcccg tttccctgtc tctcagcaca ccataaaaca aatccgcttg
     2761 ttcagtatga ccgcgttgca gcatatagtt ttgcatggca actaactgac ttccgctagt
     2821 gtacccttcg tcaatcagcg tgaccagttg ctccagcaat aacattaagt cgcgctggcg
     2881 aatatgtttt tgcagtagtt cgagaatcgc aatccgccga tgttgcatga tttcgtcatc
     2941 cggtgtgatg gtgatatcca ccagcgggaa aggactgtta tagacgcgtc gtgccagctc
     3001 cggcgagtaa aatatatcaa accagcacat tgagagcgga taaggtgtgg cctcgccctg
     3061 ataaaacagt atcggcacca ccagcggcag cttatcgtga tccgcctcca gatgccggtg
     3121 catggcagca atagaataac gcatcatgcg aaagaccatt ttcttatccg gcttgctttg
     3181 gtgttcaatc acaacatgca gataaccggg attgccctgc atttgcacgg aatagagcac
     3241 gtccgtgctg tgtcctttca ggctctcttc aatgaaactc cccgactcta aatgaagcgt
     3301 gttgaggtca caaagttctc gtaattccac tggcaaatgt atctccagaa agtcgcgagc
     3361 cgtctccgcg tgcattaaaa attgtttaaa cactgcatca tgcggtgtgg tactcggtgc
     3421 atccatcttc cctgccttcc gtgttgtctt gcgtgaaggc agtatccagt caaggcgtgc
     3481 catcgtcagc cacctcttta gtgtttaccg agcagcgttc agagaaattt caattggtta
     3541 cagccgttac atggcacgct tcggcacggt acgcaaactg ctgatgtaaa tgccgtcaga
     3601 tgagtagaca ctaaacaaaa atcgggcaat actgcgtgag aatttcctca aagcaggcat
     3661 cgcctgcttc gttaacgaca gggtagaaag gtagaagtta tgattcgcac gatgctgcag
     3721 ggcaaactcc accgcgtgaa agtgactcat gcggacctgc actatgaagg ttcttgcgcc
     3781 attgaccagg attttcttga cgcagccggt attctcgaaa acgaagccat tgatatctgg
     3841 aatgtcacca acggcaagcg tttctccact tatgccatcg cggcagaacg cggttcgaga
     3901 attatttctg ttaacggtgc ggcggcccac tgcgccagtg tcggcgatat tgtcatcatc
     3961 gccagcttcg ttaccatgcc agatgaagaa gctcgcacct ggcgacccaa cgtcgcctat
     4021 tttgaaggcg acaatgaaat gaaacgtacc gcgaaagcga ttccggtaca ggttgcttga
     4081 ttgttatccc tgcggctggt tactcaccag ccgcgacatc gtctccagcg aatccgttct
     4141 taagatataa agtcgtttta gtaacaacgg attatccccc ggttttactt tgcctttcat
     4201 ggttgtcacc gccaggtgaa atccggcatc gtttgctgcc ttcacggctt tgtcattaaa
     4261 tccgccaaac ggatacgaaa gataccagac atgcggatta aattgcgcca gagcgcggcg
     4321 tgaacgtgca aaatcaaaca gaatattgtg ctcactacgg ctcagtaata tgggtcggcg
     4381 atagccatcc acccgatgca aaaaatgggt atgtgactga aaatcaaaaa catcgcgaat
     4441 ttcgttaagc tcagaaacgc tcataaattg cagcgatttt gggttccact tctgcgggtg
     4501 acgtttgatg cgcgaggtaa caacaaacgc cgtcgccttc atgccatatt gtttcaatac
     4561 aggatacgca tagcggctca ccgacttgag gccatcatca aaggtaatca ccaccgctcg
     4621 cgcagggaga ttgatcttat tcttcacgta accttccagc tgcgccatgc tcagtgtcgc
     4681 gtatcccctg tcgcgcagcc aggccatttg gttattgaaa gcgcgtaccg aggtggtcgt
     4741 cgaagtatgg cgaaaacggg tgttttcttc gtcgcgcaga atatggtgat aagttagcac
     4801 cggaaggcca ttatcgggtt gggcatccag cgcgctgata taggccagtc gatcgccaat
     4861 acggacctga taccaggttt gatttaacct gtctttcagt ttatgcaaaa tcgggtagcg
     4921 caaattgtcc gccaataccc caaatggcgc actgcccaca ctcggcgcgt tatagaccgg
     4981 cgtatctttc caggtaacta agttctgatt actcagcggc ttgttgaggt cacccaaacc
     5041 gtcttcaact ttttgtcgcc cctgaaccgg ctcgagatgc cctttatcga taaacccttt
     5101 accaaagcca aaattaaatg cgtaataact tgcggcagtg ggctccacgg caatgatttg
     5161 tccggcccga atattcccca cggtcaccat cttgtcgcca atttgcgccc agaccgcagc
     5221 attttcgatg gtttgcatat aacgggcagg taacgcggca ctgacacttg cggtaaacag
     5281 catcagcagg agaataacag cttgtttgta catgaaaaaa taaccgaggg tgaaacaacg
     5341 cttttattgt agcaaaagcg ctataagaat cgtgggttaa tactcataca aattatgttt
     5401 gaggacgaaa ggcggatttt tttgttgttg gtgccaaaga ctactcacct ccggcccacc
     5461 cagttcgaca atacgctcgc gaaactctga actggtcatg gtttcacggc aagccatcat
     5521 ctgttcaatt aacggtaacg tgacaccaga aatcacttcg caacgggagt gtttgtggct
     5581 taataatgaa gccacgcggt acggtggcgc tccggctata tcggttaaga agatgacacc
     5641 ctcaccagag tccgcttcat gcagagcatc gcacatcatg cggctgagca tattagagct
     5701 taacccacgc cagaaattca cggcccggca ctgaagaagc gccccatgtt ttttctccag
     5761 cgcatccagt atctcttgcg cccggtcatc gtgacaggta attacccaac ctaacatcgc
     5821 cttcctcctt agccgaaaat cagtctaccc tgaccattgt tgataaaggg tgatagccgt
     5881 caaaactcct ctcccccaaa tccagaggag aggaaatagc cttagctacg caaaccacgt
     5941 ccacgttgga tcagcgacca acagatcaaa taaaacgcca caataaagac caccagtacg
     6001 ccaaaggtag tgaccagcgg aacatcattg ataccgagga agccgtagcg gaaaccactg
     6061 atcatataaa cgattgggtt caggtgcgac agcccttgcc agaacggcgg caacaaagtc
     6121 agtgagtaaa agaccccacc caaatacgtg agtggcgtta acacaaaggt tggcaccagg
     6181 ctgatgtcat cgaacgtttt ggcaaacaca ccgttcagca aacccgcaag ggagaacaac
     6241 accgccgtga gcaccagggt taaggcaacg aatacccacg aatgcacctg aaacggcaca
     6301 aaaaacagtg aaattgccgt caccagaatg ccaacaaaca gaccacgcgc cacgccaccg
     6361 ccgacatatc cggcaataat gacgtgagtc ggaaccggcg ctaccagcag ctcttcaata
     6421 ttacgctgga acttggcacc aaaaaatgat gacgcaacgt tggcgtaggc attggtgatc
     6481 accgacatca tgatcagccc cggtacgatg aactgcatat agctgaagcc atgcatatcg
     6541 ccaatacgcg aaccaatcag gttaccgaag ataataaagt aaagggtcat ggtgatgact
     6601 ggcggcacca gcgtctgcac ccagatacgc ataaagcgat ggatctcttt cgcccagatg
     6661 ctttttagcg ccacccagta aagatgcatc atgcgcgatc tccttgtttt tcattaacca
     6721 gtgaaacaaa cagctcttcc agacggttag ctttgttacg catacttaat acctgaatgc
     6781 cctgctcact taactgcgta aatacgctgt tgatcccctg ctcacgcagc acttcaactt
     6841 ccagcgtcgc ggtatcgacc agtcgatact gatagccatc gagcttcggt aacggacttt
     6901 tcggtgcgag atcgagaata aaggtttccg atttcagctt cgccagcagc gccttcatcg
     6961 aggtattttc caccagctca ccgtgttgaa taatgccgat attgcggcac agcatttctg
     7021 cttcttccag gtagtgtgtg gtgagaatga tggtggtgcc tttgtcgttt aaatccttca
     7081 aaaagcccca cattgagcgg cgaagttcaa tatccacgcc tgcggtcggt tcgtcgagaa
     7141 tcagtagttt aggttcatgc attaacgcac gggcaatcat taaacggcgc ttcatcccgc
     7201 cagataacat acgcgcacgt tcgttgcgtt ttccccatag atcgagttgt ttaagatatt
     7261 tttcgctgcg gatgtacgcc tctttgcgct ccacaccgta gtaccctgcc tgattcacca
     7321 caatttgctg cacggtttca aacgggttga agttaaattc ctgcggcacc agtcccaact
     7381 gacgtttagc gttcacgaca tctttctcga gatcgtaacc aaatacgctg acccgcccgg
     7441 aggttttatt taccagagag ctgataatac cgatagtggt cgatttcccg gccccgttcg
     7501 gcccgagaag cgcataaaaa tcacccgctt cgacctgcaa atctattcca cgaagcgcct
     7561 gaacgccgcc tggataggtt tttttaagct gttgaagttc cagtgcaatg gtcataaatt
     7621 tttacttacc ttacgttctt acactttatc tgtggtttaa atcgtcccgg agttgcccta
     7681 tattagccaa acgtaattat ttggttacag gtcgttaacc tccatgaaag acatagatac
     7741 actcatcagc aacaatgcac tatggtcaaa aatgctggtg gaagaggatc ccgggttttt
     7801 tgagaaactg gcacaagcgc aaaaaccgcg ctttctatgg attggatgtt ccgacagtcg
     7861 cgttcctgca gaacgtttaa ccggtcttga gccgggcgaa ctctttgttc accgtaatgt
     7921 tgctaacctg gtcattcaca ccgacctgaa ctgcctttcc gtggttcagt atgcagtgga
     7981 tgtactcgaa gttgaacaca ttattatctg tggccactac ggttgcggcg gcgtacaagc
     8041 cgccgttgaa aacccggaac tggggcttat caacaactgg ctgctgcata tccgcgatat
     8101 ctggttcaaa catagctcat tgctcggcga aatgccgcaa gagcgccgtc tggatacctt
     8161 gtgtgaactg aacgtcatgg aacaggttta taacctaggc cactccacca ttatgcaatc
     8221 agcgtggaaa cgcgggcaga aagttaccat tcacggctgg gcctacggca ttcacgacgg
     8281 cttgctgcgt gatctggatg tcacagcgac caaccgcgaa acccttgagc aacgttaccg
     8341 tcacgggatt tccaacctca agctgaaaca cgccaaccac aaataaaaat gccatgccgg
     8401 atgcaacaca tccggcaact tcacacttac tcgtcccgca gaatcacttt gccgatatac
     8461 ggcagatgac ggtaacgctg tgcgtaatca atgccgtaac ccaccacaaa ctcatccggg
     8521 atcgagaaac cgataaattc taccgggacg ttcacttcac gacgggacgg tttatccagc
     8581 agcgtacaaa tcgccagcga cttcggttcg cgcaggctta agatctcacg cactttcgac
     8641 agtgtattcc ccgagtcgat gatatcttca acaatcagca cgtccttgcc acggatatct
     8701 tcatccagat ctttgaggat tttcacatca cgggtggtgg acatgccgct accgtagctg
     8761 gaggcggtca taaagtcgac ttcatgagat acctgaactt cacggcacag gtccgccata
     8821 aacataaatg agccacgcag cagacccacc agcaccatat cgctgccgct gtctttgtaa
     8881 cgctcagtaa tctgacgacc cagttcggcg atacgcgctt taatctccgc ttcggggatc
     8941 attacttcta cagtatgttt catatctcta accatatgat ttaaaaacaa atcacttcgc
     9001 acgggcaggt tttggcaccg caacaaagcg caagacacgc agtataccag taaaactatt
     9061 cattcgcgga atctgttaat aaagctcgaa ttgtgatgac ggtcacacat gttaaaacct
     9121 ataattaatt gctactaaaa tattaatgaa ttgaaatggt gtctctttat ggcaattaac
     9181 aatacaggct cgcgacgatt actcgtcacg ctaacagccc tttttgcagc gctttgcggg
     9241 ctgtatctac tcattggcgg aggctggctg gtcgcgattg gcggctcctg gtactaccct
     9301 atcgctggcc ttgtgatgct cggcgtcgcc tggatgctgt ggtgcagtaa acgcgccgcg
     9361 ctttggctat acgcagccct gctgctcggc accatgattt ggggcgtctg ggaagttggt
     9421 ttcgacttct gggcgctgac tccgcgcagc gatattctgg tcttcttcgg catctggctg
     9481 atcctgccgt ttgtctggcg tcgcctggtc attcctgcca gcggcgcagt tgccgcactg
     9541 gtggtggcac tgctgattag cggtagtatc ctgacctggg ccggatttaa cgatccgcag
     9601 gaaatcaacg gcaccttaag cgccgatgcc acacctgcgg aagctatctc acccgttgcc
     9661 gatcaggact ggcctgccta tggtcgcaat caggaaggtc aacgcttttc acctctgaaa
     9721 caaattaacg ccgataacgt ccataatctg aaagaagcct gggtgttccg tactggcgat
     9781 gtgaagcagc cgaacgatcc gggcgaaatc accaatgaag tgacgccgat taaagtgggc
     9841 gacacccttt acctgtgtac cgctcaccag cgcctgtttg cgctcgatgc cgccagcggc
     9901 aaagagaaat ggcattacga tcctgagctg aaaactaacg agtctttcca gcacgtaacc
     9961 tgccgtggcg tctcttatca tgaagccaaa gcagaaacgg cttcgccgga agtgatggcg
    10021 gattgcccgc gtcgtatcat tcttccggtc aatgatggtc gactgattgc gattaacgct
    10081 gaaaacggca aactgtgcga aaccttcgcc aataaaggcg tgctcaatct gcaaagcaat
    10141 atgccagaca ccaaaccggg tctgtatgaa ccgacttcgc caccgattat caccgataaa
    10201 accatcgtga tggccggttc agttaccgat aacttctcaa cccgcgaaac gtctggcgtg
    10261 atccgtggtt ttgatgtcaa caccggggag ctgctgtggg cttttgatcc cggcgcgaaa
    10321 gatccgaacg caatcccgtc tgacgaacac acctttacct ttaactcgcc aaactcctgg
    10381 gcaccagcgg cctatgacgc gaagctggat ctggtctatc tgccgatggg cgtgaccacg
    10441 ccggatatct ggggcggtaa ccgcacaccg gaacaggaac gttatgccag ctcgattctg
    10501 gcgctgaatg ccactaccgg gaaactggcg tggagctacc agaccgttca ccacgacctg
    10561 tgggacatgg atcttccggc acagccgacg ctggcggaca tcaccgttaa tggtcagaaa
    10621 gtgccagtta tttacgctcc ggcgaaaacc ggcaacattt ttgtgctcga tcgtcgcaat
    10681 ggcgaactgg tggtttcggc cccggaaaaa ccggtgccgc aaggtgctgc gaaaggcgat
    10741 tacgttactc cgacccagcc gttctctgaa ctgagcttcc gtccgaagaa agatttgagc
    10801 ggtgcggata tgtggggagc caccatgttt gaccaactgg tgtgccgcgt gatgttccac
    10861 cagatgcgct atgaaggcat tttcacgccg ccatctgaac agggcacgct ggtcttcccg
    10921 ggtaacctgg gaatgtttga atggggcggg atttccgttg atccaaatcg tgaagtggca
    10981 attgccaacc caatggcact gccgtttgtt tcgaaactga tcccacgcgg tccgggtaac
    11041 ccgatggagc agccgaaaga tgccaaaggc acgggtacgg aatccggcat tcagccacag
    11101 tacggtgtac cgtatggtgt cacgctcaac ccgttcctct caccgtttgg tctgccgtgt
    11161 aaacagccag catggggtta tatctcggcg ctggatctga aaatcaatga agtggtgtgg
    11221 aagaaacgta ttggtacgcc gcaagacagt atgccgttcc cgatgcctgt accggtgccg
    11281 ttcaatatgg gtatgccgat gctgggcggg ccaatctcca cggcgggtaa cgtgctgttt
    11341 atcgctgcta cggcagataa ctacctgcgc gcttacaaca tgagcaacgg tgaaaaactg
    11401 tggcagggcc gtttaccagc gggtggtcag gcgacgccaa tgacctatga agtgaatggc
    11461 aagcagtatg tggtgatctc cgcaggcggt cacggttcat ttggtacgaa gatgggcgac
    11521 tatattgtgg cttatgccct gccggatgat gtgaaataac cataatgccc ggagagatcc
    11581 gggcatattt ccgaatacgg tctttttata ccgtaaaccc taacatcatc cccgtatctt
    11641 catgctccag cagatggcag tgcgccatat aagcatgttc tttcggtgcg ttgtgattaa
    11701 acttcaccag cacttcactg acattgcctt ctaccttaac ggtatctttc cagcccgcgc
    11761 gatgagccgc tggcggtttg ccattttctg acaagatacg gaactgcatg ccgtgaatat
    11821 ggaacggatg cagcatcatg tcgcccacgc cagagataac ccaacgttcg tactgccctt
    11881 tcgccgccgc aaacatcggc ttattcatat caaacgcctg accgttgatt ttattggcat
    11941 ggtggaaatc gaacttcccg ccgtggttca tatgattcat attgccgtgc cccatatggc
    12001 ccatcatctg gctgtgatcc attccggcca tcgcctgatc gccatatttc tccattagca
    12061 tctgcatccc catcatatcg agcatcgggt ccatagaaag ttgcagcttg cgtaccgtca
    12121 gcccttccag cgaaggtaac gcaggcaggc tacttaatgt gtcaggcaaa gcaccggagg
    12181 cactaatagc aatcggctga atccgcatta ccggatgcgg cttgtcaaac ggcgcaatcg
    12241 ccatccccat ctggctgacc ggtagcgtca ccagatcaaa gggtttgttg tcgttaacct
    12301 ccaccagcac ttcaaaacgc tcgcccatca gcaccggcag ctcgctcacc ttcaccggtt
    12361 caggtagcag accaccgtcg ctggcaatca catacagcgg gcgattgtcg ctggtggcga
    12421 aattgagcga gcgggcgtta cagccattga gcaaacgcag gcgcagccag ccacgcgggg
    12481 cagcgtgttg tgggtagatt gcaccgttgg tcagcagcgt atcgccaaac cagcccacgg
    12541 cggcggtcat cacatccagt tgataatcaa tctgcccatc agcgttaaat tttttatcct
    12601 gaacaatcac cggaacatca tcgatacccc actgttttgg cagcattaat ttcaggatct
    12661 cgtcatcttc aattaccacc agcccagcca accccatcgc cacctgtcgc ccggtcttgc
    12721 catgttgatg tggatggaac cagcaggtag cggcaggttg atcaacgttc aacgtcaccg
    12781 tacgtttgcc gcctggcgga ataattccct gcgggccgcc gtcgacgtca cccggtactt
    12841 ccagcccgtg ccagtgcaac gtcgtctctt ccgccagttg gttatagata tcaaccgtta
    12901 ccgctttgcc gcgctgtaat ttcaccgccg gccccagcag attgccgtta tagccccagg
    12961 tagttgcagt tttctcgcca aaggtagact gacctgcgcc aatagttaac tgaatgcgat
    13021 tacgggcatc ggtcgtgagc aaatcaggga tcggtaacgt tgggcgttcc gccgcaaaga
    13081 ctgcgcggct ccacagcggc aaggctgaag ccacacccag cgcgacggaa tattttaaga
    13141 aatcacgacg ttgcatagtt atttccttat tcttaagcag gcgaaacaaa atcaaacgtt
    13201 gagcatagtc cttcccctta cgggaaggtc aagccgcaga cacaatttaa tcgacaataa
    13261 tggtcgtcgc ctcgctcgca gtgtgctaac gtttatcttc tttaagcccc tggtagaagc
    13321 aatgaagacg tttttcagaa cagtgttatt cggcagcctg atggccgtct gcgcaaacag
    13381 ttacgcgctc agcgagtctg aagccgaaga tatggccgat ttaacggcag tttttgtctt
    13441 tctgaagaac gattgtggtt accagaactt acctaacggg caaattcgtc gcgcactggt
    13501 ctttttcgcc cagcaaaacc agtgggatct cagtaattac gacaccttcg acatgaaagc
    13561 cctcggtgaa gacagctacc gcgatctcag cggcattggc attcccgtcg ctaaaaagtg
    13621 caaagccctg gcccgcgatt ccttaagcct gcttgcctac gtcaaataat ccctgatacc
    13681 tttttgtaga aatattgacc gtgcatccgc ggtcaatgtt agctattatg ttgcgccctt
    13741 tttttacggg tgttaacaaa ggaggtatca acccatggcc gaaaaaaaac agtggcatga
    13801 aacgctacac gaccagtttg ggcagtactt tgcggtagat aacgttctgt atcatgaaaa
    13861 gaccgatcac caagatctga tcatttttga gaacgctgca tttggtcgcg taatggcgct
    13921 ggatggcgta gtacaaacca ccgagcgcga cgagtttatc tatcatgaga tgatgaccca
    13981 tgttccgcta ctggcccacg gtcacgcgaa acatgtgctg attatcggcg gcggcgacgg
    14041 tgccatgctg cgtgaagtaa cccgacataa aaacgttgag tcaatcacga tggtggaaat
    14101 cgatgcgggt atcgtgtcgt tctgccgtca gtatctgccc aaccataacg ccggtagcta
    14161 cgacgatccg cgctttaagc tggtgatcga cgatggcgtc aatttcgtta atcaaaccag
    14221 ccagaccttt gatgtcatta tctccgactg caccgatcct atcggtcccg gcgaaagtct
    14281 gttcacctca gcattttatg aaggctgcaa acgttgcctg aatcctggcg gtatcttcgt
    14341 cgcacaaaac ggcgtctgct ttttacagca ggaagaagcc atcgacagct atcgcaaact
    14401 cagccattac ttcagcgacg ttggctttta tcaggcggcg atcccgacct attacggcgg
    14461 tatcatgact tttgcatggg cgacagataa cgacgcctta cgccatctct caaccgaaat
    14521 tattcaggcg cgttttctcg cttctggcct gaaatgccgt tattacaatc cggcaatcca
    14581 tacggcagct tttgccttac ctcagtatct gcaagacgca ctggcttcac agccgtccta
    14641 aggagaagat aagaaattga aaaaactgaa actgcatggc tttaataatc tgaccaaaag
    14701 tctgagtttt tgtatttacg atatctgcta cgccaaaact gccgaagagc gcgacggtta
    14761 tattgcttat atcgatgaac tctataatgc caaccgtctg accgaaatcc tgtcagaaac
    14821 ctgttccatt atcggggcca atattcttaa catcgcccgc caggattacg aaccacaggg
    14881 tgccagcgtc actattctgg tgagtgaaga accggttgac ccgaaactca tcgacaaaac
    14941 agaacacccc ggcccactgc cagaaacggt cgttgcccat ctcgataaaa gtcatatttg
    15001 cgtacatacc tacccggaaa gtcatcctga aggcggttta tgtaccttcc gcgccgatat
    15061 tgaagtctct acctgcggcg tgatttctcc gctgaaggcg ctgaattacc tgatccacca
    15121 gcttgagtcc gatatcgtaa ccattgatta tcgcgtgcgc ggttttaccc gcgacattaa
    15181 cggtatgaag cactttatcg accatgagat taattcgatt cagaacttta tgtctgacga
    15241 tatgaaggcg ctgtatgaca tggtggatgt gaacgtctat caggaaaata tcttccatac
    15301 caagatgttg cttaaagagt tcgaccttaa gcactacatg ttccacacca aaccggaaga
    15361 tttaaccgac agcgagcgcc aggaaattac cgctgcgctg tggaaagaaa tgcgcgagat
    15421 ttattacggg cgcaatatgc cagctgttta acggctctgg cggagctccc aggctccgcc
    15481 agatttattt acttctgctg cacgaaattg cggtaagccg ccacgacctg cagaaaatcc
    15541 tcaacgccgc atagcgacag gctttcttcg tcgtagtagt tcatcccttc ttccatttca
    15601 tcgccagcga attccagttg attggcgcga accatcacct cttcaccgtc catccacagg
    15661 gtgtattcat gccctgcccg ttgccaggac cgttcgctac ctttcagtgc gtgcgctgct
    15721 tgttccactt catcaagcaa agccaggttt tctttcacct cttcattaaa ccagtgcccg
    15781 accacttcat gccccatgga catacgcacc tttaccactc cggtaatatc gcgcagaaat
    15841 tcgtaatcca tagtgtgttc ctctgctccc ggcaatgcgc cgaaatcgca ttgcgctatt
    15901 gatgtaatta ttgcagcctt accggagaaa aacagcggaa cggcgatggt ttagaaataa
    15961 aaaatgcccg ggaatctcat tcaccgggca ttgtgtcgtt tatgcgcagc gcgtgcgctt
    16021 acttattaca ccgcagtctg gaaaatcacc ccatcggctt tttcggtgta ctgagaaagc
    16081 tggttgaagt tcagataacg ataagtatca acggcagttt tatctacttg cgcaacgtag
    16141 gtctggtact cttccggcgt cggcagtttg ccaatcagcg ccgcaacagc cgccagttct
    16201 gcagaagcca ggaagacatt cgcgccagtc cccagacggt tcgggaagtt acgggtagag
    16261 gtggaaacca ccgtcgcacc gtctgctaca cgcgcctggt tacccataca cagagaacag
    16321 cccgggatct cgatacgagc cccgctctta ccgaagacgc tgtagtagcc ttcttcagtc
    16381 aactgtgcgg cgtccatgcg ggttggcggt gccacccaca ggcgggtcgg caactggcct
    16441 ttgtgcgcat ccagcagttt acccgcagca cggaagtgac cgatgttagt catgcaagaa
    16501 ccgataaaca cttcgtcgat cttctcgccc tgtaccgcag acagcggacg tgcgtcgtcc
    16561 gggtcgttcg gtgcacagag gattggctct ttaatatccg ccagatcgat gtcgatcact
    16621 gccgcgtatt cagcatctgc atcggcttcc agcagctcag gattcgccag ccatttttcc
    16681 atgccctgaa tacgacgttc gagggtacga cgatcgccat aaccttccgc gatcatccac
    16741 ttcagcagga cgatgttaga gttcaggtat tcgatgatcg gttctttgtt cagcttgata
    16801 gtacaaccag cggcagaacg ctcagcggac gcatcggtca gttcaaatgc ctgctcaact
    16861 ttcagatccg gcagaccttc aatttccaga atgcgaccag agaagatgtt tttcttgcct
    16921 ttcttctcaa cggtcagcag accttgtttg atcgcataca gcgggatcgc gtgtaccaga
    16981 tcgcgcaggg tgatgcccgg ctgcattttg cctttgaagc gcaccagaac ggattccggc
    17041 atatccagcg gcattacgcc tgttgcggct gcaaatgcca ccagaccaga acccgccggg
    17101 aaagagatac cgatcgggaa acgggtatgg gagtcaccac cggtaccgac ggtatccggc
    17161 agcagcatac ggttcagcca ggagtggatt acgccgtcac ccggacgcag cgacacacca
    17221 ccacggttca taatgaagtc cggcagcgtg tggtgcgtgt tcacgtcaac tggcttcgga
    17281 tacgccgcgg tatggcagaa agactgcatc accaggtcag ccgagaagcc caggcacgcc
    17341 aggtctttca gttcatcacg ggtcatcggg ccggtggtgt cctgtgaacc tacagaagtc
    17401 attttcggtt cgcagtacgc gcccggacga atacctttca caccgcaggc acgacctacc
    17461 attttctgag ccagcgagaa gccgcgatcg ctctcagcga catctttcgc ctgacggaac
    17521 acatcactgt gcggcagacc aagtgcttca cgcgctttgg tggtcaggcc acgcccgata
    17581 atcagcggaa tacggccacc agcacgcact tcatcaatca gcacgtcggt tttcagttcg
    17641 aaggtcgcca gcagttcgcc ggtttcgtgg ttacgcactt cacctttgta cgggtaaacg
    17701 tcaatcacgt cgcccatgtt caggttagag acgtcgactt cgattggcag tgcacccgcg
    17761 tcttccatcg tgttaaagaa gatgggtgca attttaccgc cgaggcacaa accaccgccg
    17821 cgtttgttcg gcacatgtgg aatatcatcg cccataaacc acagaacgga gttcgtggcg
    17881 gatttacgcg atgaacccgt acccacaacg tcaccgacgt acgccagcgg gaaacctttc
    17941 tgttgcagag cttcgatttg cttgatcggg ccaacaacac ctggctggtc tggctcaata
    18001 ccttcacggg cgtttttcag catcgccagc gcgtgcagtg ggatatccgg gcgcgaccac
    18061 gcatccggtg ccggagaaag gtcatcagtg ttggtttcgc cagtgacttt gaagacggta
    18121 acggtcagtt tttcagccag cgccgggcga ttcaggaacc attcggcatc cgcccaggac
    18181 tgcattacct gcttcgcata ttcgttgcct gctttggctt tctcttctac gtcatagaag
    18241 ttatcgaaca tcagcagtgt gtgagaaagt gctttggcag cgatcggtgc cagtttggca
    18301 tcatccagcg cgtcgatcag cggatgaatg ttgtaaccac cctgcatggt acccagcagt
    18361 tcgatggctt tttccggagt cagcagaggg gatttggctt cgcctttagc gatagcagcc
    18421 aggaagcctg ctttgacata ggcggcttca tcgacgccag ggggaacacg gttggttaat
    18481 agatctaaca ggaattcttc ttcgcccgcg ggcgggtttt tcagcagctc tacaagtgcg
    18541 gccatttggt ttgcatccag gggtttgggc gcaatcccct cagcggcacg ctcagctacg
    18601 tgcttacggt attcttctag cacgacggtt ctcctcgctc tcattgtcat agtgcggcag
    18661 gcgattctct tcacgctcct gtgagacagc agtttgtagg gtaaatgccc agtaccgcaa
    18721 cgggcagaat agcaggattt tgacgacgtg ttaatctgtt tacaaaaaag caacataaaa
    18781 aagtggctga atcgttaagg atggtctaga gattgtttcc ctccgtaaaa acttcgttaa
    18841 ttcgcatggt gataatcacc tttctcaacg aatcccaaga ttagtcaaaa tttaaactac
    18901 cgcctcttta tactcggatt aacagcacct gcgggtggca gttcgccgac cattgcgatt
    18961 tccttgagat ccgaattatg aaaatgactt tgccgtttaa accccatgtg ctggcactaa
    19021 tttgcagtgc cgggctttgt gccgcctctg ccgggctata tataaaaagc cgcacagtgg
    19081 aagcgcctgt ggaaacgcaa tcgacacaac agactgcgcc tgacatcacc gcagttacgc
    19141 ttcctgcaac ggtttccgca ccgcccgtaa cgcccgccgt cgtcaaatcc gcattcagca
    19201 ctgcacaaat cgatcaatgg gttgcgcctg tcgcgctgta ccccgattct ctgctttcac
    19261 aagtgttaat ggcatcaacc tatccggcaa acgttgctca agcagtgcaa tggtcgcacg
    19321 ataatccact taaacaaggc gatgctgcta ttcaggcggt atctgaccag ccgtgggacg
    19381 ccagcgttaa atcactggtg gcctttccac aattgatggc attgatgggc gaaaacccgc
    19441 aatgggtgca aaacctgggc gatgcttttc tggcgcagcc gcaggacgtg atggactcgg
    19501 tacagcgatt gcggcaactg gcgcaacaaa ccggctcgct gaagtcatca accgaacaga
    19561 aagttattac cacaacgaag aaagctgtac cggtaaaaca gacagtcacg gcacccgtca
    19621 taccatccaa taccgtttta actgccaacc ccgtcattac agagccggca acaaccgtca
    19681 tttccattga gcccgccaat cctgatgtgg tctatattcc caactacaac ccaaccgtgg
    19741 tttacgggaa ctgggccaat actgcgtatc cgccggttta tctgccacca ccagccggag
    19801 aaccgtttgt tgacagcttt gtgcgcggat tcggctatag catgggcgtt gctaccacgt
    19861 acgcactatt tagcagcatc gactgggatg acgacgatca tgaccatcat catcatgacg
    19921 atgatgatta tcatcaccac gatggcggtc atcgtgacgg taatggctgg caacacaacg
    19981 gcgacaacat caatatcgac gtcaacaatt tcaaccgtat caccggtgag catcttactg
    20041 ataagaatat ggcatggcgg cacaatccaa actaccgtaa tggtgtgccc tatcatgatc
    20101 aggatatggc aaagcggttt catcaaaccg atgtcaacgg cggaatgagt gccacgcagc
    20161 tacctgctcc aacacgcgac agccagcgtc aggcggcagc aagtcagttt cagcaacgaa
    20221 cacacgccgc ccccgtcatt acacgagata cccaacgtca ggcagcggca cagcggttta
    20281 atgaagctga acactatggg agctatgacg acttccgcga cttcagccgt cgccaaccac
    20341 tgacccagca acaaaaggac gccgctcgtc agcgttatca atcggcctct cctgagcagc
    20401 gccaggcggt tcgcgagaaa atgcagacta acccacagaa ccagcagcga agagaggcag
    20461 cgcgtgagcg tattcagtct gcaactcccg aacagcgcca ggtgtttaag gaaaaagtac
    20521 agcagcgccc actgaaccaa cagcaacgtg ataacgcccg ccagcgtata caatcggcat
    20581 cacctgaaca acgtcaggtt tttcgggaaa aagttcagga gagccgccca caacgtctaa
    20641 acgacagtaa ccatactgtc aggctgaata acgagcaacg gtcagcagta cgcgaacgtc
    20701 tctctgagcg cggagcaagg cgacaggaaa ggtaaattgc agacgtaaaa aaagcggcgt
    20761 ggttagccgc ttttttaatt gccggatgtt ccggcaaacg aaaaattact tcttcttcgc
    20821 tttcgggttc ggcaggtcgg taatgctacc ttcgaacact tctgccgcca ggcccacaga
    20881 ctcgtgcaga gtcgggtgcg cgtggatggt cagtgcgatg tcttcagcat cacaacccat
    20941 ttcgattgcc agaccgattt cacccagcag ctcaccgccg ttagtaccga caatcgcacc
    21001 accgatcaca cggtgagatt ctttgtcgaa aatcagcttg gtcataccgt ctgcgcagtc
    21061 ggaagcgata gcacgaccag aagcagccca cgggaaggtg gcggtttcat agctgatgcc
    21121 tttctctttc gcttctttct cagtcagacc tacccatgca acttctggtt cggtataggc
    21181 gatagacggg ataactttcg gatcgaagta gtgtttttta ccggcgataa cttcagcggc
    21241 aacgtgacct tcgtgaacac ctttgtgtgc cagcatcggc tgaccaacga tatcgccgat
    21301 agcaaagatg tgcggtacgt tggtacgcag ctgtttgtca acgcggatga aaccacggtc
    21361 gtccacttcc acgccagctt tgcctgcgtc gaggttttta ccgttcggca cacgaccaat
    21421 cgctaccagc acggcgtcgt aacgctgcgg ttcagcgggt gcttttttgc cttccatcgt
    21481 cacataaata ccgtcttctt tcgcttcaac ggcggtaact ttggtttcca gcatcaggtt
    21541 gaatttcttg ctgatacgct tggtgaagac tttaacgatg tctttgtcag ctgccgggat
    21601 aacctggtcg aacatttcaa ccacgtcaat ctgtgaaccc agcgcgtggt atacggtgcc
    21661 catttccaga ccgatgatac cgccacccat taccagcagg cgttctggta cttctttcag
    21721 ttccagcgcg tcagtggagt cccagatacg cggatcttca tgcggaataa acggcagttg
    21781 gatcgggcga gaacccgctg caatgatcgc attgtcgaag ttgatcacgg ttttgccgtt
    21841 ctcaccttca acttccaggg tgttagcccc ggtgaattta cccagaccgt tgaccacttt
    21901 gactttgcgg cctttcgcca taccagccag accaccggtc agctgattga tcactttctc
    21961 tttccaagta cgaatcttgt caatatcggt tttcggttcg ccgaagacga taccgtgttc
    22021 agccagcgct ttggcttctt cgataacttt tgctacgtgc agcagtgctt tagaagggat
    22081 acagccgacg ttcaggcaaa caccgccaag ggtgttgtaa cgttctacga ttacggtttc
    22141 cagacctaaa tcagcgcaac ggaaggcagc ggagtaacct gcggggcctg ccccaagtac
    22201 cacgacctga gttttgattt cagtactcat catgacctct atatatttat ctccggcggt
    22261 catacccgtc gtctttcagg ccgcagcggc gttagcttca cgccctcacc ccagtcactt
    22321 acttatgtaa gctcctgggg attctggcgc ttgctacctt gctgcaccca gaaatccata
    22381 gggtatgtga agtaataccc taaccaccac cgggtcgttc tatccgtcgg tattttacaa
    22441 aattgttaac aatttttaaa caacaaacgg caaccgattt gtctattcgc taataacctc
    22501 aatacattca tgagattacc agaaaaaagc cggccgttgg gccggctctt ttacttacat
    22561 caccagacgg cgaatgtcag acagcgtgtt gttaatgatg gtaatgaaac gggcaccatc
    22621 agcaccgtcg atcacgcggt ggtcgaagga gagagaaatc ggcagcatca gacgcggcac
    22681 gaactcttta ccattccaca ccggctccat cgcggacttg gaaacgccga ggatagccac
    22741 ttccggcgcg ttcacaatcg gcgcgaagtg ggtagtaccc aggccgccga tgctggagat
    22801 ggtgaagcaa ccgccctgca tttcgcccgc agtcagctta ccgtcacgcg ctttcttaga
    22861 aatagtcatc agctcgcgag acagctcgat gatgcctttc ttgttgacgt ctttgaatac
    22921 cggaacaacc agaccgttcg gggtatccac cgccacaccg atgttgatgt atttcttcag
    22981 ggtcagacgc tgaccgtctt ccgacagcga actattgaag cgaggcatct gctcaagagc
    23041 tgcagcaacg gctttcatga tgaagacaac cggggtgagc ttcacatcca gcttacgttt
    23101 cgccgcttct tcgttctgct gtttacggaa cgcttccagc tcggtgatat cggttttgtc
    23161 gaagtgggta acatgcggga tcatcaccca gttacggctc aggttagcac cagaaatttt
    23221 ctggatacgg cccagttcca cttcttcgat ttcgccaaac ttgctgaagt ccaccttcgg
    23281 ccacggcagc atgccaggga taccaccgcc agtcgccgcc ggagctgctt ctgcacgttt
    23341 gatagcttct ttcacgtaag cctgaacgtc ttcgcgcagg atacgacctt tacgaccagt
    23401 gcccttcact ttcgccaggt taacgccaaa ctcgcgtgcc agacggcgga tcagcggagt
    23461 cgcgtgaacg taagcgtcgt tttcagcaaa ttcagatttg ccttcggctt tcgcagccgg
    23521 agctgctgcc ggggcttcag ctttcgctgc cggagccggc g
//
LOCUS       DAJTZL010000051        23237 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.051, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000051 DAJTZL010000000
VERSION     DAJTZL010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23237
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.051"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(40..240)
                     /gene="glgS"
                     /locus_tag="OO544_001757"
     CDS             complement(40..240)
                     /gene="glgS"
                     /locus_tag="OO544_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HCR6767374.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
     gene            509..1138
                     /locus_tag="OO544_001758"
     CDS             509..1138
                     /locus_tag="OO544_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1449 family protein"
                     /protein_id="HCR6767375.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEILALICGHMLSGALDAHLD
                     HYNSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHACIMVWQSPLSNLF
                     VVPVSLLFTIIAVHYTGKIVAPWIPRDHSSAITEEEYIGSMALITGHQATSGNPCEGK
                     LTDQFGQIHYLLLEPEEGKIFTKGVKVLIICRLSATRYLAENNPWPQIL"
     gene            1165..2826
                     /locus_tag="OO544_001759"
     CDS             1165..2826
                     /locus_tag="OO544_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HCR6767376.1"
                     /translation="MDDIVNSVPSWMFTAIIAVCILFIIGIIFARLYRRASAEQAFVR
                     TGLGGQKVVMSGGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFF
                     VRVKPSVEGIATAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENF
                     VQGVQNTVAEDLSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLTQETERRRR
                     ERNEVEQDVEVAVREKNRDALSRKLEIEQQEAFMTLEQEQQVKTRTAEQNARIAAFEA
                     ERRREAEQTRILAERQIQETEIEREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKS
                     IAIAAKSEQQSQAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETK
                     AVELTVRAKAEKEAAEMQAAAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLK
                     FKLALLQALPAVIEKSVEPMKSIDGIKIIQVDGLNRGSAAGDANTGNVGGGNLAEQAL
                     SAALSYRTQAPLIDSLLNEIGVSGGSLAALTSPLTSTTPVAENVE"
     gene            3065..3124
                     /locus_tag="OO544_001760"
     CDS             3065..3124
                     /locus_tag="OO544_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCR6767377.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            3441..3500
                     /gene="ibsE"
                     /locus_tag="OO544_001761"
     CDS             3441..3500
                     /gene="ibsE"
                     /locus_tag="OO544_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HCR6767378.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(3620..5053)
                     /gene="hldE"
                     /locus_tag="OO544_001762"
     CDS             complement(3620..5053)
                     /gene="hldE"
                     /locus_tag="OO544_001762"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HCR6767379.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPSSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKQGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(5101..7941)
                     /gene="glnE"
                     /locus_tag="OO544_001763"
     CDS             complement(5101..7941)
                     /gene="glnE"
                     /locus_tag="OO544_001763"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HCR6767380.1"
                     /translation="MKPLSSPLQQYRQTVVERLPEPLAEEPLSAQAKSVLTFSDFVQD
                     SIIAHREWLTELESQPPQADEWQHYAAWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSCSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMNNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLISDFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALNHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNDREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSTEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGRTLQTEVREMREKMRAHLGNKHRDRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSGDCFTAERDLVRASWQKWLVEE"
     gene            complement(7964..9265)
                     /gene="ygiF"
                     /locus_tag="OO544_001764"
     CDS             complement(7964..9265)
                     /gene="ygiF"
                     /locus_tag="OO544_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HCR6767381.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRRHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQFPSEV
                     WPNGELPADLASRVQPLFSTDFYREKWLVAVDGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELALAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSHHAAELKSVFCQPLGDLYRDQLPRLTRDIDSILLLAGYYDPVVAH
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            9507..10127
                     /locus_tag="OO544_001765"
     CDS             9507..10127
                     /locus_tag="OO544_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HCR6767382.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            10191..11429
                     /gene="cca"
                     /locus_tag="OO544_001766"
     CDS             10191..11429
                     /gene="cca"
                     /locus_tag="OO544_001766"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HCR6767383.1"
                     /translation="MKIYLVGGAIRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTDFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(11592..12413)
                     /gene="bacA"
                     /locus_tag="OO544_001767"
     CDS             complement(11592..12413)
                     /gene="bacA"
                     /locus_tag="OO544_001767"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCR6767384.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDISMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(12504..12872)
                     /gene="folB"
                     /locus_tag="OO544_001768"
     CDS             complement(12504..12872)
                     /gene="folB"
                     /locus_tag="OO544_001768"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HCR6767385.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAM
                     ARAANVGVIIERGNNLKENN"
     gene            12977..13594
                     /gene="plsY"
                     /locus_tag="OO544_001769"
     CDS             12977..13594
                     /gene="plsY"
                     /locus_tag="OO544_001769"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HCR6767386.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(13607..14539)
                     /gene="ttdR"
                     /locus_tag="OO544_001770"
                     /gene_synonym="dan"
                     /pseudo
     CDS             complement(13607..14539)
                     /gene="ttdR"
                     /locus_tag="OO544_001770"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TtdR"
     gene            14746..15657
                     /gene="ttdA"
                     /locus_tag="OO544_001771"
     CDS             14746..15657
                     /gene="ttdA"
                     /locus_tag="OO544_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HCR6767387.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATVKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIIPDNDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLTFENLSHTRSAL"
     gene            15654..16259
                     /gene="ttdB"
                     /locus_tag="OO544_001772"
     CDS             15654..16259
                     /gene="ttdB"
                     /locus_tag="OO544_001772"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HCR6767388.1"
                     /translation="MKKILTTPIKAEDLQDIRVDDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            16308..17771
                     /gene="ttdT"
                     /locus_tag="OO544_001773"
     CDS             16308..17771
                     /gene="ttdT"
                     /locus_tag="OO544_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HCR6767389.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALIPVPAGLESHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLIVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(17814..18827)
                     /gene="tsaD"
                     /locus_tag="OO544_001774"
     CDS             complement(17814..18827)
                     /gene="tsaD"
                     /locus_tag="OO544_001774"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HCR6767390.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            19064..19279
                     /gene="rpsU"
                     /locus_tag="OO544_001775"
     CDS             19064..19279
                     /gene="rpsU"
                     /locus_tag="OO544_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HCR6767391.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            19390..21135
                     /gene="dnaG"
                     /locus_tag="OO544_001776"
     CDS             19390..21135
                     /gene="dnaG"
                     /locus_tag="OO544_001776"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCR6767392.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            21330..23171
                     /gene="rpoD"
                     /locus_tag="OO544_001777"
     CDS             21330..23171
                     /gene="rpoD"
                     /locus_tag="OO544_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HCR6767393.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
BASE COUNT         5653 a         6332 c         5906 g         5346 t
ORIGIN      
        1 ctaatacaaa cactaaaacg ggccatcagg cccgttatat cagtgctcta actccagctc
       61 tcttgcctgc atcatctgtt ggcaatacca ggcataacgt gcgcaaaacc aggttctatg
      121 ttcttcatcc atgttaccag taacggcaag aatatcgacc gggcgacctt ctgcgtgcat
      181 tctggcaaaa ctacgcgcca ggaaatcgaa attatttaaa gaattaagac tatgatccat
      241 aagcacatcc tccggttatc gcataccttt taaggccata tgctttttct gatttcagta
      301 ttccccaatc agtgtagtgc ctttaaacat aacgtgcgta aatatattgt ccccgatcac
      361 actttttagt gaaaaataaa aaactatatt aaaaaacttt ctcttccaga acttctctaa
      421 catgtgttat tattaacaaa gataaatgta agcaaggaaa ctatcccaaa gtaaatattg
      481 cgtcactaaa tggacattgg agtgacatat gattttattc gccgactata atacccctta
      541 tcttttcgct atttcctttg tactgttgat cggcctttta gaaattttag cgcttatctg
      601 cggtcacatg ctctccggtg ctcttgatgc acatcttgac cattataatt ccataaccac
      661 aggtcatatt agccaggcac ttcattatct caatatcgga agattgccag ctctcgtcgt
      721 tctctgcttg ctggctggtt tttttggtct tattggcatc ctgttgcagc atgcctgcat
      781 catggtctgg caatcaccac tgtcgaacct gttcgtggtt cctgtcagtt tactgtttac
      841 gattattgcg gtgcattata ccggtaaaat agttgcgccc tggattccac gcgaccatag
      901 ttcagctatt acagaagaag aatatattgg tagcatggca ttaattaccg gtcatcaagc
      961 cacttcaggt aatccctgcg aaggaaaact caccgatcaa tttggccaaa tacattattt
     1021 actactggag cctgaagaag gaaaaatttt cactaaagga gtcaaagtgt taattatttg
     1081 ccgactttct gcaacgcgat atttggcgga aaacaatccc tggccacaaa tattgtaatt
     1141 tattaatcaa aaggaaattt aataatggat gatattgtta attctgtgcc ctcctggatg
     1201 tttaccgcga ttattgccgt atgcattctg tttattattg gaattatttt cgccaggctc
     1261 tatcgtcgcg cttcggcaga gcaagctttt gttcgtactg gtttaggtgg gcaaaaagtg
     1321 gtgatgagcg gtggcgcaat cgtgatgccg atctttcatg aaataatccc catcaatatg
     1381 aatactctga agctggaagt cagccgctca accattgata gcctgattac gaaagatcgt
     1441 atgcgtgtcg atgtagtagt cgctttcttt gtacgggtaa aaccgtctgt agaaggcatc
     1501 gccactgcgg cgcagacgtt ggggcaacgc acgctatcac cagaagactt acgtatgttg
     1561 gttgaagata aatttgtcga tgccctccgt gcaacagctg cacaaatgac catgcatgag
     1621 ttacaggata cccgcgagaa cttcgtgcag ggagtacaaa atactgtcgc agaagaccta
     1681 tcgaaaaacg gtcttgagct ggaaagcgtt tcacttacca actttaacca gacctcgaaa
     1741 gaacatttca atccgaacaa tgcctttgac gccgaaggtt taaccaaact gactcaggag
     1801 acggagcgcc gtcgtcgcga acgtaacgaa gttgaacagg atgtagaagt tgcggtgcgt
     1861 gagaaaaacc gtgatgcact ttcgcgcaag ttggagattg aacaacaaga agcgtttatg
     1921 acgcttgagc aggagcagca ggttaaaacc cgtactgccg aacagaatgc acgtattgcg
     1981 gcttttgaag ctgaacgtcg tcgtgaagca gagcagacgc gaattctggc tgaacgacaa
     2041 attcaggaaa cggagatcga gcgcgagcag gccgtccgct caagaaaggt tgaggctgaa
     2101 cgtgaagttc gtattaaaga gatcgaacag cagcaggtca ccgaaatcgc caaccagacg
     2161 aaatcgatcg ctattgccgc caaatcggaa caacagtccc aggcagaagc gcgtgctaac
     2221 cttgcactcg cagaagcagt aagtgcgcaa caaaacgtag aaaccactcg ccagaccgca
     2281 gaagccgatc gtgctaaaca agttgcccta atcgctgccg cgcaggatgc agaaaccaaa
     2341 gcggttgaac tgaccgtgcg ggcgaaagca gaaaaagaag ccgcagagat gcaggcggcg
     2401 gctatcgttg agttagccga agctacacgt aaaaagggtc tggcggaagc agaagcacaa
     2461 cgtgcgctga acgatgctat caacgtactt tctgatgaac aaaccagcct taaattcaaa
     2521 ctggccttgt tgcaggcgct acctgcggta atagagaaat ccgttgagcc gatgaagtcc
     2581 attgacggta tcaagattat tcaggtcgat ggtctgaatc gtggcagcgc tgcgggtgat
     2641 gcaaacacgg gtaatgtggg gggcggaaac ctagcggaac aagcattatc agccgctctc
     2701 tcttaccgca cacaggcacc gctgattgac tccttgctca atgaaattgg cgtttcaggc
     2761 ggctcactgg cggcattgac ttcaccctta acctcaacaa ctcccgtcgc cgaaaacgta
     2821 gaataaattt tcagctatga acgtccctgg cacaaaaagc cggggatttt ttatatctgc
     2881 gttccgctaa aaactgcaaa tgatcaggcc gttgcaacgt tttgcgtgac cgctcgggga
     2941 aggcaaaact gcctctggga aagcgttgcg cgaggtccgg cgctcatcaa caatcgggga
     3001 gtcagcaaag ggctgaaacg ggaaagcccc tcccgaggaa ggggccttac agaaggaaag
     3061 ggttatgatg aagctcgtca tcatactgat tgtgttgtta ctcgtaagtt tcgcagctta
     3121 ttaacagcca atcagagggg ggagaaatcc tccctcaccc ttgttccttt actttaggtt
     3181 gaaaaaaaat cagcgtcaag aaggtttcat taacacatat ccctggccca aaaagccggg
     3241 gattttttat atctgcgttc cgctaaaagg tgcaaatgct caggacgttg cagcgttttg
     3301 cgtgatcgct cggggaaggc aaaactgctt cagggaaagc gttgcgcggg gtccggcgct
     3361 catcaacaat cggggagtca gcaaggggct gaaacgggaa agcccctccc gaggaagggg
     3421 ccttatagaa ggaaagggtt atgatgaagt tcgtcatcat actggttgtg ttgttactgt
     3481 taagtttccc gacttactaa caactgatca gagggggaag aaatcctccc ttacccttgt
     3541 tcctttactt taggttgaaa aaacaacagc gtcaataggc ctgcctgcta caaagcgaga
     3601 tctgtgaacc gctttccggt tagccttttt tatcctgttg gatcttcttg atgatgttag
     3661 tggtcgagca accgtcttcg aagttgagca ccagcacttc gccgccgttg gcccagactt
     3721 ctttactccc ggcgatctct tctggtttat agtcgccgcc cttcaccagt aaatctggca
     3781 ggatcccggc gatcaagcgc tgcggcgtgt cttcttcaaa cgacaccacc cagtcgaccg
     3841 cttccagtgc gcccagcaca atcatacgct gttcgagcgg gttaacgggg cgggaatccc
     3901 ctttcagccg tttggtggag gcatcgctgt tcacagcgac aatcagacgg tcaccctgtt
     3961 tgcgagcatt tgccagatag gagacgtgcc cggcgtgcag gatgtcaaag acaccgttgg
     4021 tcattaccac cttttcgccg cgtttacgcg ctgccgctac ggccagcttc agttcctctt
     4081 cggtcatcac gccaaagccg gtatctgcgc gcccacgcac tgcattttcc agctcgatcg
     4141 gcgaaaccgt ggaggttccc agtttgccga ccaccacgcc agccgccgca ttggcaaaga
     4201 agcaggcttc ttccagcgaa ttacccgctg ccagcgttgc cgccaggacg ccaatcaccg
     4261 tgtcgcccgc accggtaacg tcatacactt cctgcgcctg ggttggcata tgcagcggcg
     4321 ctttacccgg ttgcagcagc gacataccct gttcggaacg ggtcactaac agagccgaga
     4381 gttcgtaatc ggcaatcagt ttcatgccgc gctcaacaat ctcttcttcg gtcttacatt
     4441 taccgacaac agcttcaaat tccgagagat tcggcgttaa cagcgtagcg ccgcggtagc
     4501 gctcaaaatc ggtacctttt ggatcaatca gcaccggaac gcccgcttta cgcgccagtt
     4561 ggatcatctg ctgtacgctt gccagcgcac ctttggcgta gtcagaaagc accagcgcgc
     4621 caatcgaact cagcgcctga ttaatccgct cgtgcagcgg ctgcggatca acgccttcga
     4681 aaccttcttc gaaatccaga cggatcagct gttggttgcg ggaaagtacc cgtaatttgg
     4741 tgatggtcgg atgcgtcggt acagaaacga agtcgcattt gacgttgacg tcggccagag
     4801 atttactcag cgcgcgcgct gcatcgtcaa tgcccgtcaa cccgaccagg cgtgcattag
     4861 caccgagaga agcgatattc atcgccacgt tagccgcgcc gcccggacgt tcttcgatgg
     4921 tattcacttt aaccacgggc accggcgctt ccggcgagat acggctggag ggaccgtacc
     4981 agtaacgatc cagcattaca tcaccaacca ccatcactcc tgcacgttca aactctggca
     5041 gcgttacttt cattcctgtc tcctgagaga ttcaaaattt gcgcgcgata ataccatact
     5101 tcattcttcc accagccact tctgccagct tgcccgcacc agatcacgct ctgcggtgaa
     5161 gcaatccccc gacacatggc ccggcaattc ctgtaatgcc agatgatgaa gttcatcgcg
     5221 cagcgtcgtg taagcacggg tcagcgccat cgcttcctgc tcttccataa tgtcgttttg
     5281 cgccagcagc tccagaatac gcacgttgtc tgaccagcgc gttaacttcg gtttttcatg
     5341 agcgtagcgc aacaccagat attgggtaat aaattcgata tcggtaattc cgccttcatc
     5401 agctttgata tcaaagcgat cgcgatgttt attgccgaga tgagcgcgca ttttctcgcg
     5461 catttcccgc acttccgttt gcagagttct accttcacgc ggcagcgtca taatctcgcg
     5521 acgcactgcg tcaaagtgcg cggtgagctg cggatcgccg tacactaccc gcgcacgcac
     5581 cagcgcctga tgttcccacg tccaggcctc gtttttctga taatcggcaa atgcttctgt
     5641 ggaggtaacc agcattcccg ccgccccgga cgggcgcagc cgcgcatcca cttcgtacaa
     5701 aatgccggaa gaggttcgcg tgctgaacag gtgcataatg cgttgcgcca gacgaagata
     5761 aaactgtcgt ccgtcgattt cccgctcgcc gtcagtcatc gcatccattg ggcaatcatg
     5821 gaggaagatc aggtcaagat cggaactgta gcctaactcc cagccgccca gcttgccgta
     5881 gccgaccact gcaaaaccac gcccttcgcg atcgttgagg tgattcggct taccgtagcg
     5941 ggcaaccatt tgaacccacg cctgctgaac gacggcatct atcatggctt ccgccagcca
     6001 ggttaagtga tcgctcactt tcatcaccgg tagcgtacca gcgatatccg ctgcggcgat
     6061 gcgtaacagc tgcgcctgtt tgaactgacg cagcgcctca agctgttgct cttcgtcatc
     6121 ttccggcacg cgcagcaaat actggcgcaa ctcatcgcgg taggcatcgg tcgccgtcgg
     6181 ctggtaaaga gtgtttggat cgagcaattc atccagcaat aatggataac gcgccagctg
     6241 gctggcaatc atcggcgatg cggcacacag agaaatcaaa tggttaagcg ccgcagggaa
     6301 ttcactgagc aattctaaat aggtggtgcg ggtaacaatc cccaccagca aggcggtaat
     6361 gcgcgacagc gtgacagcag cgtcttcacg cgcacagaca tcacttagca gatgcggcat
     6421 cagatggtcg agcacctgac gtcctcgcgg cccgatggtg cgcttatcca gctctttgcg
     6481 gaaatcggaa atcagcgtta gcacctgttt gcgatcatcc tcgctaagat gcgccagcac
     6541 tggcgtagtg tcatcttcct gcaacgcatc ctgccacagc tcacgccact gttccgacag
     6601 cgactcttcc tgagtttcac tttcgtcgtc gccaatcaat tcattaaaca cccggcgcac
     6661 attgttcata tgtgcggtca gcgccccggt cagttgcggc cagtcagcaa agtccatcgc
     6721 ccacgccagc cgcgcacggt taagctcatc agaaggaagc gtctgggttt gttcgtcgtt
     6781 aatgctttgc agcaggtttt ccagacgccg caggaacaga tacgccactc gcaattgttc
     6841 agcatcgttt tcagaaagca gatgcagcgc agcaatggcg ctgagcgttg gcagtaaaga
     6901 gcacgattgc agcgacggtt cgcgcccgcc gcgaatgagc tggaatacct ggacgataaa
     6961 ttcaatttcg cgaataccac ccgcgccaag tttgatgttg tcggtcaaac cgcgccgacg
     7021 cacttcacgg gcaatcattc ctttcatgtt gcgcagcgac tgaatcacgc tgaaatcgat
     7081 gtaacgacgg aaaacaaacg ggcgtagcat cgcacgcaac tcgttagcat agacgccttc
     7141 gctatcgccc ataatccgcg ctttgaccat cgcgtaacgc tcccagtcgc gcccctgctc
     7201 ctggtaataa tcctccaacg cggcaaagct cagcaccagc gggccacttt cgccaaacgg
     7261 acgcagtcgc atatccacgc gatagacgaa gccatcctgc gttggttgat ccagcacttt
     7321 aatcagccgc tgccccatgc gggtaaaaaa ctgcgcgtta tccagttccc ggcgtccgcc
     7381 ctgcgtacaa ccatgttccg gccaggcaaa aatcagatcg atatcagagg agaaattcag
     7441 ttccccgccg cccagcttac ccatgcctaa gatcagcagc ggttgcgctt cgccctgcgc
     7501 attgcacggc gttccccact cgcggcagca ggcgtcatac agccagtcac gcgccgcaac
     7561 aatcagcgtc tccgccagat aactgagctg ctgcaatatg ctctcttcag taaccagtgc
     7621 cagcgtttgc gcccatgcga tgcgcaccat aatgcgccgc cggaatagcc gcagctcgcg
     7681 cattaacccg gcttcgtcac tcacattact gagcgcctcc tgcaaccatg cagcgtaatg
     7741 ctgccattca tcggcctgcg gcggttggct ttccagttcc gtcaaccact ctcgatgcgc
     7801 aattatgctg tcctgcacaa aatcactgaa agtaagtact gacttcgcct gtgcacttag
     7861 tggttcctcg gctaaaggct ctggcagccg ctcaacaacg gtctgcctgt actgctgtaa
     7921 cggtgaagag agcggcttca taagcgattt tatccttgcc tgattaacgt tttccgctgt
     7981 gcaaccagaa cggttcctga ttgtttgcct cattacggaa atgctcaatt tcaatgcgtt
     8041 gcccggtcgc aatagcgtga cgcagcccct gccagttctc cagccaggca tgcgcgacga
     8101 caggatcata gtaacccgcc agcaacagta ttgagtcaat gtcacgcgtc aggcgtggca
     8161 actggtcacg gtagagatcg cctaacggct ggcagaaaac gcttttcagt tcagcggcat
     8221 ggtgggaaag atggatatcg gcaaagcgtt tgaaggagtc gctgattttg ccctgcgctt
     8281 tggcatctaa aaatggctgc catgctttgc ttaccaacca ttcggtcaac gccagcttcg
     8341 ccattgccgt ttcggtagag tagaccgccg tcacggcaga aaccgcagaa gcaatggtcg
     8401 cttcgcattg ggtcagcaaa tcacgtaagt gagtgctcgc tttacgcggc acaataccac
     8461 cgaacagcat cagcgtatga cggaccaggc taatggctgc cagcacctgt tctttcgccg
     8521 catcgttacc gcgtacccac agttcttcat gatactgcca ttgcgctaac gccagctcga
     8581 gcgccgcttc cagcccctgt tctacatcgg cttttgccgc aacatgcaaa atggtggtcg
     8641 gtttgatttc acgcgccgga ttgccctgcg ccagatgata gccacgcgcc gctttgctca
     8701 ggctgccctg gcgtaatccg gtttgcgata ccagttggtt cgccagtttc agcaccgcgc
     8761 gcgtgtcgcc gctaagcagt tccagttcca gctcacagat aggttcagca aattcccccg
     8821 ctttcacctc cccctgatcg agagcgattt caattcggct gccatcgacc gccaccagcc
     8881 atttttcgcg ataaaaatcg gtgctgaaca gcggttgtac gcgggaagcg agatcggcag
     8941 gcaattcacc gttcggccag acttccgagg gaaactgcgc caggtcgagc gtcggttcgc
     9001 tcaacgccac gttatattcc gggcgctggt gtaagccgcc tgtcactctt cctgcaactt
     9061 tcatggtcat ctcatagcga ccgttttcgc cacgaatacg taagcccata tcgtgcctac
     9121 gcagccagtt atccggcgtt tcgtagtaaa tattcagcaa ctgcacgggg tcatggtgct
     9181 cgccgcccag cgtattgaga tggtcacgca acgcctcaac ggcactgtga ttaacaataa
     9241 actttaattc gatttcctga gccatggcct ttacttatcg gttatgtcac ataagtgacg
     9301 atgaacggcg aacttaatgc gatctttttt gtcagtagat agtattttgc gccaaattgc
     9361 catgcaacgg gcaatttgac gggcgtaaaa gtttgaagca gtggcaagac ctgacacaga
     9421 ggattccgat tgatgtcgaa tgctttgcgt agcgacactg atgccactac tatcgttcca
     9481 ctttccatga caataacgac agcctgatgc caaaattacg cctgatcgga ttaactttac
     9541 tcgcacttag cgcgactgcc gtctcacacg ctgaagaaac gcgctatgtt tctgacgaac
     9601 tgaatacctg ggtccgtagc ggtccgggag atcattatcg cctcgtgggc acggttaacg
     9661 ccggcgagga agtgacctta ttacaaactg acgccaacac caattatgcc caggtgaaag
     9721 acagctctgg ccgtaccgcc tggatcccgt tgaaacaact tagcactgag ccaagcctgc
     9781 gctcccgtgt gccagatctg gaaaatcagg tcaaaaccct gaccgataaa ctcaccaata
     9841 tcgataacac ctggaatcag cgcaccgcag aaatgcagca aaaagtggcg cagagcgaca
     9901 gcgtgatcaa cgggttaaaa gaagaaaatc agaaactgaa aaacgagctg attgtcgcgc
     9961 agaaaaaggt cgatgccgcc agcgtacagc tggatgacaa acagcgcacc atcatcatgc
    10021 aatggtttat gtatggcggt ggcgtgctgg ggcttggctt gctgctcggt ctggtactgc
    10081 cgcacctgat cccaagccgc aaacgcaaag atcgctggat gaactaaatc gccttcttcg
    10141 ttgcactgtc ttattattag gaattgtgta tttgacgaag ggatgatggc gtgaagattt
    10201 atctggtcgg tggtgctatt cgggatgcat tgttagggct accggtcaaa gacagagatt
    10261 gggtggtagt cggcagtacg ccacaggaga tgctcgacgc gggctaccag caggtaggcc
    10321 gcgattttcc tgtgtttcta catccgcaaa cgcatgaaga gtatgcgctg gcacgtaccg
    10381 aacggaaatc cggttccggt tacaccgatt ttacttgcta tgccgcaccg gatgtcacgc
    10441 tggaagatga tcttaagcgt cgcgatctga ccattaatgc gctggcccag gacgataacg
    10501 gcgagattat cgacccgtac aacggtctgg gcgatctgca aaatcgtctg ttgcgccatg
    10561 tttcccccgc ttttggcgaa gatccgttac gcgtactgcg cgtggcgcgt tttgctgcgc
    10621 gttatgccca cctcggtttt cgtattgccg atgaaactct gacgctgatg cgcgagatga
    10681 cccatgcggg tgaactggaa cacctgacgc ctgaacgggt atggaaagag acggaaagcg
    10741 ccctcaccac ccgcaatcca caggtgttct tccaggtact gcgcgattgc ggcgcactgc
    10801 gcgttttatt cccggaaatt gacgcactgt ttggcgttcc ggcccctgcc aggtggcatc
    10861 cggaaatcga cacgggtatt cataccttaa tgacgctctc aatggcggcg atgctgagtc
    10921 cgcaggtcga tgtccgtttc gcaactttat gccacgatct cggtaaagga ctgacgccgc
    10981 cagaactttg gccgcgtcat catggtcacg gtccggcggg tgtgaaatta gtggaacaac
    11041 tctgccagcg tctgcgcgtg ccaaatgaaa ttcgcgattt agccagactg gtggctgagt
    11101 ttcacgatct catccacacc ttcccaatgc tgaacccaaa aactatcgtc aaattgtttg
    11161 attccatcga cgcctggcgt aaaccgcagc gtgtcgagca actggcgctg accagcgagg
    11221 ctgacgtacg cggcagaacc ggttttgaat cagcggacta cccgcaaggc cgttggttgc
    11281 gcgaagcctg ggaagtggcg cagtcagtgc cgacaaaagc ggtcgttgaa gcgggattta
    11341 aaggtgtgga gattcgcgag gagctgaccc gacggcggat tgcggcggta gccagttgga
    11401 aggaacaacg ttgcccaaag cctgactgag tataaaacta aacgttaaca cgaaccatcc
    11461 cctcgcccct tcggggagag ggttagggtg aggggaacag accagcaccg gtgcgaacaa
    11521 tcacccccac cccggccctc tccctacaga gcgagggaga aaaccaggcc tgctcccgaa
    11581 ccttttctca atcaaaagaa cacgacgtac accgcagccg ccacaataaa gcgataaatg
    11641 gcgaacggga taaacgaaat gcgcttaatc aattgcagga aggttttaat cgctatcagc
    11701 gccaccacaa aagcggtgat aaacccaacg gcaaacatcg agatatcgcc ggttgtcagg
    11761 aagccccagc ttttgtagag atcgagcgcc gttgcgccca tcatcatcgg taccgccagc
    11821 aggaacgaga actcggaagc agcgtaacgg ctcacgccca tcagcatccc acctgaaatg
    11881 gtggccccgg aacgggagaa acccggccac agcgccagac actggaaaca gccaatcata
    11941 aacgcctgac gataggtcat atcatcaaga cccggcgcac gcggctcttt cggcttcagg
    12001 cattcggcgg caatcagcaa caaaccgcca acgaccagtg catacatcac atttatcggg
    12061 ttaaacaatg acttaatcgt gtcgtggaac aacagcccca gtaccaccgc cggaatcatc
    12121 cccagcaaaa tgtggatcag cgttaaacga cctttgcttt ctccttcgtg ctgcaacggg
    12181 cggccaaagt ggatgccaat caggccaaac agacgccgcc agaacatcac tactaccgcc
    12241 agaattgatc ctaactggat cacaacttca aaggttttcg ccgtgtcgcc ctcgaacccc
    12301 aacaagtgac cgacaataat catatggccc gtgctggata ccggcagaaa ttctgtcaat
    12361 ccttcgacca cacccaatat tgccgctatc agcagcgagt gcatatcgct catcaataaa
    12421 cccctaaatt attaaaatgt accgcttgtc cggactactg cagtaagacc aggttataac
    12481 cgtttggttt aacagctgta aaattaatta ttttctttca gattattgcc acgctcaatg
    12541 attacaccaa cattcgccgc ccgcgccatt gcgcctggct tgctgagttt gatgcgcacc
    12601 cacggcgagt tgaagcgtgc taacagcagc tccgccacct cttcagccac gcgttccacc
    12661 agcgcaaaac gcgccccctc gacgtggctg accaccgttt ctgcaatgtc agcgtaactg
    12721 aggcaatccg ccacatcatc acttttcgcc gctttacggt tatcccatgc catttcgata
    12781 tcgaacacta acttctgttc gatggtctgt tcccagtcgt aaacaccaat agtggtgatt
    12841 accgaaagtt gctctataaa tacaatatcc atcacgtcct gcctgctttt tggctaaccg
    12901 gataccactt ccggcgaaat gtgcgtatta tccacagatt catcgttgaa cacgaatttt
    12961 caaaacggaa cagcttatga gtgcaatcgc gcctggaatg atcctcatcg cgtacctctg
    13021 cggctccatt tccagtgcca ttctggtttg ccgcttgtgt gggctgcccg atccgcgaac
    13081 cagcggctcc ggcaatccag gcgcaaccaa tgtgttacgt atcggtggca agggagcagc
    13141 cgtagcagta ctgattttcg acgttctgaa aggaatgttg cccgtctggg gcgcgtatga
    13201 attaggtgtc agccccttct ggttaggctt aattgccatc gccgcctgtc ttggacacat
    13261 ctggcccgtt ttcttcggat ttaaaggagg aaaaggcgtt gctaccgctt ttggtgccat
    13321 cgcacccatt ggctgggatc tcaccggagt aatggcggga acctggttac tgaccgtgct
    13381 attgagcgga tactcgtcgc tgggagcgat tgtcagtgca ctgattgctc cgttttatgt
    13441 ctggtggttt aagccacaat tcaccttccc ggtttcgatg ctctcttgcc tgatcctgct
    13501 gcgtcatcat gacaacatcc agcgtctgtg gcgtcgtcag gagacaaaaa tctggacgaa
    13561 attcaaaaga aagcgcgaaa aggatcccga gtgatttctg gtggatctac atgacctgat
    13621 agccttcatc gggcttgccc agccgttgct ggcaccatgc cgccagaaat tccacgcaga
    13681 cacgtaattt catgctgcga tagagcggct cccggtaaac agcccagata ttggcgcttt
    13741 gtgcatactc cggcaatact tgcaccagtt tgccactctc cagaaacggc aacacatccc
    13801 actcggaacg cagcataatc cctttgccct ccagcgccca ttgcaacact atctcgccgc
    13861 tattggagga aagatgcccg ctaaccttca ccgatttttt ctcctgaccg ttccccaact
    13921 cccatattcc gtgggtcata tcgcgctctt tggtcaccag acagtcatga cgacttaatt
    13981 cctgtaatga ttgtggctgc ggatattttt gcagatactc aggtgctgcg cataatattc
    14041 ttttattttt cgttaacaga tgcgcaatat aataatcagg aatttcgtca ttaatacgaa
    14101 tatccagatc aatattatcc tgcactaaat caatttgccg atcgaacaat tcaaaatgca
    14161 cctgtaactc aggataattg cgcataagtt cggtaatggc tggcgcaata tggctgcgcc
    14221 caaaaccaaa actgcaacca atacgaatca tcccctccgg gcgcgttttg atttacgtga
    14281 cgtcatcgac cagtcgctga tattgggtga ggatttccag cgcatgttcg taacaacgtt
    14341 ggccactttc agtcagcgcc acgccgcgag cggagcggtt cagcagcgtt gttgccagcg
    14401 tattttcaag gatttggatc cgcttggtga caaatgcagg cgtctgccca agcgtcgccg
    14461 ctgcggcgct aaaactcccg ctgtggacga tttctaccag tacctgcaag tctttggcta
    14521 aaggccagct attcagcatc gttaattatc cgcagttgtg ataagcgcag tgtatttcgc
    14581 aaaacattgc cattccctgc tctggcgcaa tattcattct tattttcgcg ttgattcacg
    14641 aattgttaat tacatattca cggtggcaaa aaatataaaa ccacattttt agtggtagtt
    14701 tgtggcggtg aatttttcca gacaaataca aaaactggag ttgccatgat gagcgaaagt
    14761 aataagcaac aggcagtgaa taagttgacg gagattgtcg ctaactttac cgccatgatt
    14821 tctacccgaa tgcctgatga cgtggtggat aaactaaaac agctaaagga tgccgaaacg
    14881 tcgtcgatgg ggaaaattat ctaccatacg atgttcgaca acatgcaaaa agcgattgac
    14941 ctgaatcgtc ctgcctgtca ggacaccggg gagattatgt tcttcgttaa agtcggttcc
    15001 cgcttcccac tgcttggcga gctgcaaagc atactcaaac aagccgtgga agaggcaacc
    15061 gtcaaagcgc cactacgtca caatgcggta gaaatttttg acgaagtaaa caccggcaaa
    15121 aataccggta gcggcgtacc gtgggtcacc tgggacatca tccccgacaa tgacgatgcg
    15181 gaaatcgaag tttacatggc aggcggcggc tgcacgctac ctggccgctc gaaagtgtta
    15241 atgccgtcag aaggctacga aggcgtggtg aaattcgtct tcgaaaatat ctccaccctc
    15301 gccgtaaacg cctgtccacc ggtactggtg ggcgtgggca tcgccacctc ggtggaaacc
    15361 gccgccgtac tctcgcgtaa agccattttg cgcccgattg gcagccgcca tcccaatcca
    15421 aaagcggcag aactggagct acgcctggaa gaaggactca accgtctggg gattggtcca
    15481 caagggctga ctggcaacag ttcagtgatg ggcgtacata tcgaatctgc cgcccgccat
    15541 ccgtcaacca tcggcgttgc tgtctctacc ggctgctggg cgcatcgtcg cggcacactg
    15601 ctggttcatg ccgatctcac ctttgaaaat ctgtctcaca cccggagcgc gttatgaaaa
    15661 agatcctgac aaccccgatc aaagctgaag atctgcaaga tattcgcgtc gacgatgtga
    15721 tctacctgac cggtacgctg gtgacctgcc gtgacgtttg tcaccgccgc ttgatcgaac
    15781 tgaaacgtcc gatcccttac gatctcaacg gcaaagcgat tttccacgct ggccccatcg
    15841 tgcgcaaaaa cggcgataaa tgggagatgg tttccgttgg cccgacaacc agtatgcgta
    15901 tggaaagttt tgaacgtgaa tttatcgagc agaccggcgt gaaactggtg gttggcaaag
    15961 gcggaatggg gccgctgacc gaagaaggct gccagaaatt caaggcgcta catgtgattt
    16021 tcccggcagg ctgcgcggtg ctggcggcaa cccaggtgga agagattgaa gaagtgcact
    16081 ggacagagct cggaatgccg gagtcgctgt gggtctgtcg ggtcaaagag ttcggcccgc
    16141 tgattgtctc tattgatacc cacggcaaca acctgatagc cgaaaacaaa aagctgttcg
    16201 ccgaacgccg cgatcccatc gtggaagaga tctgcgagca cgtccattac atcaaataac
    16261 cctcccggag aggctcgccc cctctccttt ttcgcaggca taacacgatg aaaccttcca
    16321 ctgaatggtg gcgatacctt gcgccgctgg cggtcatcgc cattattgct ctaattccgg
    16381 ttcccgcagg gctggagagt catacctggc tctactttgc cgtttttact ggcgtgatcg
    16441 ttggactgat cctcgaaccc gtgccgggtg ccgtggtggc gatggtgggt atatccatca
    16501 tcgccatact ctctccctgg ctgctgttca gcccggagca gctcgctcag ccaggcttta
    16561 aattcactgc aaaatccctc tcgtgggccg tttccggttt ttctaattcg gttatctggc
    16621 tgattttcgc cgcctttatg tttggcacag gctatgaaaa aaccgggctt ggacgccgca
    16681 ttgcgctgat tctggtgaaa aagatgggac atcgcacgct gtttctcggc tatgcggtga
    16741 tgttctccga gctgatcctc gcacctgtaa caccgtccaa ctcggcgcgt ggtgcgggga
    16801 ttatctatcc catcatccgt aacctgccac cgctctatca atcacaacca aacgacagca
    16861 gttcgcgcag cattggctcg tacatcatgt ggatggggat tgttgccgac tgcgtgacca
    16921 gcgccatttt cttgacggcg atggcaccaa acttgctgtt aattggactg atgaaaagcg
    16981 catctcacgc cacgctgagt tggggcgact ggttcctcgg gatgttgccg ctcagtattt
    17041 tgctggtgct gctggttccc tggctggctt atgtgctgta cccgccggta ctgaagtctg
    17101 gcgatcaggt gccgcgctgg gcagagacgg aactgcaggc aatgggcccg ctctgttcgc
    17161 gtgaaaaacg gatgctgggg ctgatagtag gcgcgctggt gctgtggatt ttcggcggtg
    17221 attatatcga tgccgcgatg gtcggttaca gcgtggtggc actgatgctg cttctgcgca
    17281 ttatcagttg ggacgacatt gtcagtaata aagcggcgtg gaacgttttc ttctggctgg
    17341 cctcgcttat caccctcgct accggactca acaacaccgg ttttattagc tggtttggca
    17401 aactgttagc aggcagctta agcggttatt cgccaacgat ggtgatggtg gcgttgattg
    17461 tggtgtttta tctgctgcgc tactttttcg ccagcgccac ggcgtatacc tccgccctcg
    17521 caccgatgat gattgccgct gcgctggcga tgccggaaat cccgctgccg gtgttctgcc
    17581 tgatggttgg cgcggcaatt ggtctgggga gcattcttac accatacgcc accggaccca
    17641 gtccgattta ctacggtagt ggttatctgc caacggcgga ttactggcga ctgggggcga
    17701 tttttgggct gatattcctc gtgttgctgg tgattaccgg cttactgtgg atgcccgtgg
    17761 tgttgcttta agtgaggacg gtgccggaaa ttgctctccg gcacccaata gttttacgca
    17821 gccggtaact ccgccagcgg ccagcgcgga cgcacgctaa cgccgagatc cgccgtcgcg
    17881 cctgctttaa accgcaccat tccggcatag gcgatcatcg cgccgttatc agtacaaaac
    17941 tccggacgcg cgtagaacac ttcgccgcgg cgttttttca tcatttcagc cagcttcgcc
    18001 cgtaacgtgc ggttagcact cacgccgccc gccatgacca gtcgcttaaa gcccgtctga
    18061 tccaacgctc gcttacactt aatcatcaac gtatcgacca ccgcatcttc aaaggcgcgg
    18121 gcgatatcag cacgcgtctg gtcgtcggtg ccgttgtcac gaatcgtgtt cgccgcaaag
    18181 gttttcagac cagaaaagct gaaatccagc cccggacggt cggtcatcgg acgcgggaaa
    18241 acaaagcgcc cggcagtacc ctgagccgcc attttcgaca gtaacggtcc gccaggataa
    18301 tccagcccca gcagcttcgc ggttttatca aacgcttcac cggcggcatc atcgatagac
    18361 tcgccgagca gctcgtactg accaatgcca gtcacgctga ttaactgcgt atgaccgccg
    18421 gacaccagca gcgcgacaaa cggaaattcc ggcgggttat cttccagcat cggcgctaac
    18481 agatgccctt ccatatggtg taccgggatt gccggaacgt tccaggcaaa cgccagagaa
    18541 cgccccacgg ttgcgccaac cagcagtgcg ccgactaatc cagggcctgc ggtataggcc
    18601 acagcatcaa tgtcttttgc cgttaagcca gactccttta gcgccgcctg gatcaacggt
    18661 acggttttgc gcacgtgatc gcgggaggcc agttcaggca cgacgccgcc atagtcagcg
    18721 tgcaatttca cctgactata caattggttg gctaacaaac ctttttcatc gtcgtaaatg
    18781 gcgatgccgg tttcatcgca ggaagtttca atacccagta cacgcatgac ttgttttacc
    18841 tcgctttatt accgcgcagt gtaggaccaa tgcgggttga tgtaaaactt tgttcgcccc
    18901 tggagaaagc ctcgtgtata ctcctcaccc ttataaaagt ccctttcaaa aaggccgcgg
    18961 tgctttacaa agcagcagca attgcagtaa aattccgcac cattttgaaa taagctggcg
    19021 ttgatgccag cggcaaaccg aattaatcaa aggtgagagg cacatgccgg taattaaagt
    19081 acgtgaaaac gagccgttcg acgtagctct gcgtcgcttc aagcgttcct gcgaaaaagc
    19141 aggtgttctg gcggaagttc gtcgtcgtga gttctatgaa aaaccgacta ccgaacgtaa
    19201 gcgcgctaaa gcttctgcag tgaaacgtca cgcgaagaaa ctggctcgcg aaaacgcacg
    19261 ccgcactcgt ctgtactaat tccccgagag cgttgctctc cgatcagacc gagttgtagt
    19321 tgtaaggccg tgcttccgaa aggaatgcgc ggcttatttt cgtttatgaa ttgctaaaaa
    19381 tcggggccta tggctggacg aatcccacgc gtattcatta atgatctgct ggcacgcact
    19441 gacatcgtcg atctgatcga tgcccgtgtg aagctgaaaa agcagggcaa gaatttccac
    19501 gcgtgttgtc cattccacaa cgagaaaacc ccgtcattca ccgttaacgg tgagaaacag
    19561 ttttaccact gctttggatg tggcgcgcac ggcaacgcga tcgacttcct gatgaactac
    19621 gacaagctcg agttcgttga aacggtcgaa gagctggcag caatgcacaa tcttgaagtg
    19681 ccatttgaag caggcagcgg ccccagccag atcgagcgcc atcagaggca aacgctttat
    19741 cagttgatgg acggtctgaa tacgttttac caacaatctt tacaacaacc tgttgccacg
    19801 tctgcgcgcc agtatctgga aaaacgcgga ttaagccacg aggttatcgc tcgctttgcg
    19861 attggttttg cgccccccgg ctgggacaac gtcctgaagc ggtttggcgg caatccagaa
    19921 aatcgccagt cattgattga tgcggggatg ttggtcacta acgatcaggg acgcagttac
    19981 gatcgtttcc gcgagcgggt gatgttcccc attcgcgata aacgcggtcg ggtgattggt
    20041 tttggcgggc gcgtgctggg caacgatacc cccaaatacc tgaactcgcc ggaaacagac
    20101 attttccata aaggccgcca gctttacggt ctttatgaag cgcagcagga taacgctgaa
    20161 cccaatcgtc tgcttgtggt cgaaggctat atggacgtgg tggcgctggc gcaatacggc
    20221 attaattacg ccgttgcgtc gttaggtacg tcaaccaccg ccgatcacat acaactgttg
    20281 ttccgcgcga ccaacaatgt catttgctgt tatgacggcg accgtgcagg ccgcgatgcc
    20341 gcatggcgag cgctggaaac ggcgctgcct tacatgacag acggccgtca gctacgcttt
    20401 atgtttttgc cggatggcga agaccctgac acgctggtac gaaaagaagg taaagaagcg
    20461 tttgaagcgc ggatggagca ggcgatgccg ctttccgcat ttctgtttaa cagcctgatg
    20521 ccgcaagttg atctgagtac ccctgacggg cgcgcacgtt tgagtacgct ggcactgcca
    20581 ttgatatcgc aagtgccggg cgaaacgctg cgaatatatc ttcgtcagga attaggcaac
    20641 aaattaggca tacttgatga cagccagctt gaacgattaa tgccaaaagc ggcagagagc
    20701 ggcgtttctc gccctgttcc gcagctaaaa cgcacgacca tgcgtatact tatagggttg
    20761 ctggtgcaaa atccagaatt agcgacgttg gtcccgccgc ttgagaatct ggatgaaaat
    20821 aagctccctg gacttggctt attcagagaa ctggtcaaca cttgtctctc ccagccaggt
    20881 ctgaccaccg ggcaactttt agagcactat cgtggtacaa ataatgctgc cacccttgaa
    20941 aaactgtcga tgtgggacga tatagcagat aagaatattg ctgagcaaac cttcaccgac
    21001 tcactcaacc atatgtttga ttcgctgctt gaactgcgcc aggaagagtt aatcgctcgt
    21061 gagcgcacgc atggtttaag caacgaagaa cgcctggagc tctggacatt aaaccaggag
    21121 ctggcgaaaa agtgatttaa cggcttaagt gccgaagagc gatcgggaag cccccgacag
    21181 ccgcactgag aggcagcggc aaatatataa gtacgccctc gtaattatcg ttggcggtaa
    21241 acaaccgttg gatttcagcg ttaacggctg aaggacatcg ggtcaatcgc ccaacaccaa
    21301 cctcatgaaa taagtgtgga taccgtctta tggagcaaaa cccgcagtca cagctgaaac
    21361 ttcttgtcac ccgtggtaag gagcaaggct atctgaccta tgccgaggtc aatgaccatc
    21421 tgccggaaga tatcgtcgat tcagatcaga tcgaagacat catccaaatg atcaacgaca
    21481 tgggcattca ggtgatggaa gaagcaccgg atgccgatga tctgatgctg gctgaaaaca
    21541 ccgcggacga agatgctgcc gaagccgccg cgcaggtgct ttccagcgtg gaatctgaaa
    21601 tcgggcgcac gactgacccg gtacgcatgt acatgcgtga aatgggcacc gttgaactgt
    21661 tgacccgcga aggcgaaatt gacatcgcta agcgtattga agacgggatc aaccaggttc
    21721 aatgctccgt tgctgaatat ccggaagcga tcacctatct gctggaacag tacgatcgtg
    21781 ttgaagcaga agaagcgcgt ctgtccgatc tgatcaccgg ctttgttgac ccgaacgcag
    21841 aagaagatct ggcacctacc gccactcacg tcggttctga gctttcccag gaagatctgg
    21901 acgatgacga agatgaagac gaagaagatg gcgatgacga cagcgccgat gatgacaaca
    21961 gcatcgaccc ggaactggct cgcgaaaaat ttgcggaact acgcgctcag tacgttgtaa
    22021 cgcgtgacac catcaaagcg aaaggtcgca gtcacgctgc cgctcaggaa gagatcctga
    22081 aactgtctga agtattcaaa cagttccgcc tggtgccgaa gcagtttgac tacctggtca
    22141 acagcatgcg cgtcatgatg gaccgcgttc gtacgcaaga acgtctgatc atgaagctct
    22201 gcgttgagca gtgcaaaatg ccgaagaaaa acttcattac cctgtttacc ggcaacgaaa
    22261 ccagcgatac ctggttcaac gcggcaattg cgatgaacaa gccgtggtcg gaaaaactgc
    22321 acgatgtctc tgaagaagtg catcgcgccc tgcagaaact gcagcagatt gaagaagaaa
    22381 ccggcctgac catcgagcag gttaaagata tcaaccgtcg tatgtccatc ggtgaagcga
    22441 aagcccgccg tgcgaagaaa gagatggttg aagcgaactt acgtctggtt atttctatcg
    22501 ccaagaaata caccaaccgt ggcttgcagt tccttgacct gattcaggaa ggcaacatcg
    22561 gtctgatgaa agcggttgat aaattcgaat accgccgtgg ttacaagttc tccacctacg
    22621 caacctggtg gatccgtcag gcgatcaccc gttctatcgc ggatcaggcg cgcaccatcc
    22681 gtattccggt gcatatgatt gagactatca acaaactcaa ccgtatttct cgccagatgc
    22741 tgcaagagat gggccgtgaa ccgacgccgg aagaactggc tgaacgtatg ctgatgccgg
    22801 aagacaagat ccgcaaagtg ctgaagatcg ccaaagagcc aatctccatg gaaacgccga
    22861 tcggtgatga tgaagattcg catctggggg atttcatcga ggataccacc ctcgagctgc
    22921 cgctggattc tgcgaccacc gaaagcctgc gtgcggcaac gcacgacgtg ctggctggcc
    22981 tgaccgcgcg tgaagcgaaa gttctgcgta tgcgtttcgg tatcgatatg aacaccgacc
    23041 acacgctgga agaagtgggt aaacagttcg acgttacccg cgaacgtatc cgtcagatcg
    23101 aagcgaaggc gctgcgcaaa ctgcgtcacc cgagccgttc tgaagtgttg cgtagcttcc
    23161 tggacgatta atcggtaggc cggatcaggc gttacgccgc acccggcact agtccctctg
    23221 cacaaacgcc acctttt
//
LOCUS       DAJTZL010000052        23157 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.052, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000052 DAJTZL010000000
VERSION     DAJTZL010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23157
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.052"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            231..1664
                     /gene="cscA"
                     /locus_tag="OO544_001778"
     CDS             231..1664
                     /gene="cscA"
                     /locus_tag="OO544_001778"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HCR6767394.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDCAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWTFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRTISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLVLWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(1672..2730)
                     /locus_tag="OO544_001779"
     CDS             complement(1672..2730)
                     /locus_tag="OO544_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR6767395.1"
                     /translation="MDLASDVCFCYSARFTANGLGMASLKDVARLAGVSMMTVSRVMH
                     NAESVRPATRDRVLQAIQTLNYVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDIL
                     LAIEQTASEFGWNSFLINIFSEDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYG
                     ENIVLANCVADDPALPSYIPDDYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQ
                     GFEQAWRDAGRDLAEVKQFHMATGDDHYTDLASLLNAHFKSGKPDFDVLICGNDRAAF
                     VAYQVLLAKGVRIPQDVAVMGFDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGR
                     EGGRVTRIPCPLLIRCST"
     gene            2898..3116
                     /locus_tag="OO544_001780"
                     /pseudo
     CDS             2898..3116
                     /locus_tag="OO544_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            3134..4462
                     /gene="dsdA"
                     /locus_tag="OO544_001781"
     CDS             3134..4462
                     /gene="dsdA"
                     /locus_tag="OO544_001781"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HCR6767396.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFAETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNTTHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(4570..6108)
                     /gene="emrY"
                     /locus_tag="OO544_001782"
     CDS             complement(4570..6108)
                     /gene="emrY"
                     /locus_tag="OO544_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HCR6767397.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRLLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(6108..7271)
                     /gene="emrK"
                     /locus_tag="OO544_001783"
     CDS             complement(6108..7271)
                     /gene="emrK"
                     /locus_tag="OO544_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HCR6767398.1"
                     /translation="MEQINSNKKQSNRKKYFSLLVIVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLALKRTDIRSPVTGY
                     IAQRSVQVGETVSPGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKDEDIAEMPELASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            7687..8301
                     /gene="evgA"
                     /locus_tag="OO544_001784"
     CDS             7687..8301
                     /gene="evgA"
                     /locus_tag="OO544_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HCR6767399.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGILVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            8306..11899
                     /gene="evgS"
                     /locus_tag="OO544_001785"
     CDS             8306..11899
                     /gene="evgS"
                     /locus_tag="OO544_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HCR6767400.1"
                     /translation="MKFLPYIFLLCCGLWSTISFADEDYIEYRGISSNNRVILDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQRVRGINADYLNLLKRTLNIKLTLRE
                     YADHQKAMDALEEGEVDIVLSHLVASPPLNDDIAATKPLIITFPALVTTLHDSMRPLT
                     SSKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGQNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVVLNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPDLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLNPGGWDILPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIKVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDHAVYI
                     CGWQDITETRDLIHALEVERNKAINATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYSTGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALICSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLV
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEHPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFQALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPVSDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(11955..13100)
                     /gene="yfdE"
                     /locus_tag="OO544_001786"
     CDS             complement(11955..13100)
                     /gene="yfdE"
                     /locus_tag="OO544_001786"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HCR6767401.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     PGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLAKIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(13174..14118)
                     /gene="yfdV"
                     /locus_tag="OO544_001787"
     CDS             complement(13174..14118)
                     /gene="yfdV"
                     /locus_tag="OO544_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HCR6767402.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(14188..15882)
                     /gene="oxc"
                     /locus_tag="OO544_001788"
     CDS             complement(14188..15882)
                     /gene="oxc"
                     /locus_tag="OO544_001788"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HCR6767403.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(15936..17186)
                     /gene="frc"
                     /locus_tag="OO544_001789"
     CDS             complement(15936..17186)
                     /gene="frc"
                     /locus_tag="OO544_001789"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HCR6767404.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDSAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(17699..18322)
                     /locus_tag="OO544_001790"
     CDS             complement(17699..18322)
                     /locus_tag="OO544_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HCR6767405.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQTQKTAAAER
                     ISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKTNLN
                     DDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVMENQY
                     LMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            18619..18894
                     /gene="ypdI"
                     /locus_tag="OO544_001791"
     CDS             18619..18894
                     /gene="ypdI"
                     /locus_tag="OO544_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis lipoprotein YpdI"
                     /protein_id="HCR6767406.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDAPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(18971..19213)
                     /locus_tag="OO544_001792"
     CDS             complement(18971..19213)
                     /locus_tag="OO544_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HCR6767407.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            19566..20486
                     /gene="lpxP"
                     /locus_tag="OO544_001793"
     CDS             19566..20486
                     /gene="lpxP"
                     /locus_tag="OO544_001793"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HCR6767408.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNPKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            20842..20913
                     /gene="ypdK"
                     /locus_tag="OO544_001794"
     CDS             20842..20913
                     /gene="ypdK"
                     /locus_tag="OO544_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HCR6767409.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(20978..22216)
                     /gene="alaC"
                     /locus_tag="OO544_001795"
     CDS             complement(20978..22216)
                     /gene="alaC"
                     /locus_tag="OO544_001795"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HCR6767410.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDITEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
     gene            22593..>23157
                     /gene="ypdA"
                     /locus_tag="OO544_001796"
     CDS             22593..>23157
                     /gene="ypdA"
                     /locus_tag="OO544_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HCR6767411.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSDGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVSILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIV"
BASE COUNT         6485 a         5420 c         5044 g         6208 t
ORIGIN      
        1 tactttggct gacatttttt tcccttaaat tcatctgagt tacgcatagt gataaacctc
       61 tttctcgcaa aatcgtcatg gatttactaa aacatgcata ttcgatcaca aaacgtcata
      121 gttaacgtta acatttgtga tattcatcgc atttatgaaa gtaagggact atatttttat
      181 aaaagttaac gttaacaatt caccaaattt gcttaaccag gatgattaaa atgacgcaat
      241 ctcgattgca tgcggcgcaa aacgcactag caaaacttca tgagcaccgg ggtaacactt
      301 tctatcccca ttttcacctc gcgccacctg ccggatggat gaacgatcca aacggcctga
      361 tctggtttaa cgatcgttat cacgcgtttt atcaacatca cccgatgagc gaacactggg
      421 gaccaatgca ctggggacat gccaccagcg acgatatgat ccactggcag catgagccta
      481 ttgcgctagc gccaggagac gataatgaca aagacgggtg tttttcaggt agtgctgtcg
      541 atgacaatgg tgtcctctca cttatctaca ccggacacgt ctggctcgat tgtgcaggta
      601 atgacgatgc aattcgcgaa gtacaatgtc tggctaccag tcgggatggt attcatttcg
      661 agaaacaggg tgtgatcctc actccaccag aaggaatcat gcacttccgc gatcctaaag
      721 tgtggcgtga agccgacaca tggtggatgg tagtcggggc gaaagatcca ggcaacacgg
      781 ggcagatcct gctttatcgc ggcagttcat tgcgtgaatg gaccttcgat cgcgtactgg
      841 cccacgctga tgcgggtgaa agctatatgt gggaatgtcc ggactttttc agccttggcg
      901 atcagcatta tctgatgttt tccccgcagg gaatgaatgc cgagggatac agttaccgaa
      961 atcgctttca aagtggcgta atacccggaa tgtggtcgcc aggacgactt tttgcacaat
     1021 ccgggcattt tactgaactt gataacgggc atgactttta tgcaccacaa agctttttag
     1081 cgaaggatgg tcggcgtatt gttatcggct ggatggatat gtgggaatcg ccaatgccct
     1141 caaaacgtga aggatgggca ggctgcatga cgctggcgcg cgagctatca gagagcaatg
     1201 gcaaacttct acaacgcccg gttcacgaag ctgagtcgtt acgccagcag catcaatctg
     1261 tctctccccg cacaatcagc aataaatatg ttttgcagga aaacgcgcaa gcagttgaga
     1321 ttcagttgca gtgggcgctg aagaacagtg atgccgaaca ttacggatta cagctcggca
     1381 ctggaatgcg gctgtatatt gataaccaat ctgagcgact tgttttgtgg cggtattacc
     1441 cacacgagaa tttagacggc taccgtagta ttcccctccc gcagcgtgac acgctcgccc
     1501 taaggatatt tatcgataca tcatccgtgg aagtatttat taacgacggg gaagcggtga
     1561 tgagtagtcg aatctatccg cagccagaag aacgggaact gtcgctttat gcctcccacg
     1621 gagtggctgt gctgcaacat ggagcactct ggctactggg ttaacataat atcaggtgga
     1681 acaacggatc aacagcggac aagggatccg cgtcactctt cccccttcac gaccttcaat
     1741 aatatgcaat gcagcttccc gcccgataat gtcatgtgga agctgaattg tggtcagcgg
     1801 cggtaaaaac agatgcccga cgccaaccag attatcaaag cccattacgg cgacatcctg
     1861 cgggatacgt acccccttcg ccaggagaac ctgataagcg acaaaggctg cacgatcgtt
     1921 accacatatc agaacatcaa aatctggttt gcccgatttg aagtgggcat tgagtaaact
     1981 tgcgagatcg gtgtagtgat catcacctgt tgccatgtga aattgtttca cctcagccag
     2041 atctcgtcca gcatcacgcc aggcctgctc aaatccctgc cgacgatacc ctgttgccaa
     2101 cgcactttcc ggtagccaga agcataacgg ttgacgatag cccgccgcga gcaaatgctg
     2161 tgttgattca tattgtgcag tgtaatcatc agggatataa ctgggtaacg ctgggtcatc
     2221 cgccacacag ttcgccaata caatattttc accatacaga gactcaggca gcgtgatatg
     2281 tcgcagcccc attgtagtat agataatgcc atccggacgg tgggcaagca gctgacgtgc
     2341 cgcgcgggca gcgtcatctt cagaaaaaat attgattaaa aaactattcc agccgaactc
     2401 gctggcggtt tgctcaatgg caagcagaat atcaacagag aaaggagtgg tagccgtgtc
     2461 ctgcgccagc acggcgagag tcgacggctt acgtccttga gcgcgcatct tacgggcgga
     2521 aagatcagga acataattca gggtctggat tgcctgcaat acgcggtcac gcgttgcagg
     2581 acgcacagat tctgcattat gcatcacccg ggagactgtc atcatcgaca ctcccgccag
     2641 gcgtgcgaca tcctttaatg aagccatacc caagccgttt gccgtaaaac gggcactgta
     2701 gcagaaacag acgtcactgg cgagatccaa cgccctatca cctgacacag caatacaata
     2761 aaaaataaca ataattcccg gacaattgtc cccaattccg cctctgttct cgccaacgag
     2821 taaccatata ggcaagtaag taaaacatgc caactggcat cgccaacctg gagaagaaaa
     2881 tataaatatg gatcattgtg ctgccgctgt atcccgacat cagcccggaa attattgcga
     2941 ttgctatcgg ttcaggtgca attggctgca cgatcgttac ggactcgctt ttctggctag
     3001 tgaagcaata ttgcggcgct acgctcaatg aaacatttaa atactatacg acagcgacat
     3061 ttatcgcttc agtcgtcgct ctggcgggca cattcctgct gtcatttatc atctaagcgc
     3121 aaagagacgt actatggaaa acgctaaaat gaactcgctc atcgcccagt atccgttggt
     3181 aaaggatctg gttgctctta aagaaaccac ctggtttaat cctggcacga cctcattggc
     3241 tgaaggttta ccttatgttg gcctgaccga acaggatgtt caggacgccc atgcgcgctt
     3301 atcccgtttt gcaccctatc tggcaaaagc atttgctgaa actgctgcca ctggggggat
     3361 tattgaatca gaactggttg ccattccagc tatgcaaaaa cggctggaaa aagaatatca
     3421 gcaaccgatc agcgggcaac tgttactgaa aaaagatagc catttgccca tttccggctc
     3481 cataaaagca cgcggcggga tttatgaagt cctggcacac gcagaaaaac tggctctgga
     3541 agcggggttg ctgacgcttg atgatgacta cagcaaactg ctttctccgg agtttaaaca
     3601 gttctttagc caatacagca ttgctgtggg ctcaaccgga aatctggggt tatcaatcgg
     3661 cattatgagc gcccgcattg gctttaaggt gacagttcat atgtctgctg atgcccgggc
     3721 atggaaaaaa gcgaaactgc gcagccatgg cgttacggtc gtggaatatg agcaagatta
     3781 tggtgttgcc gtcgaggaag gacgtaaagc agcgcagtct gacccgaact gtttctttat
     3841 tgatgacgaa aattcccgca cgttgttcct tgggtattcc gtcgctggcc agcgtcttaa
     3901 agcgcaattt gcccagcaag gccgtatcgt cgatgctgat aaccctctgt ttgtctatct
     3961 gccgtgtggt gttggcggtg gtcctggtgg cgtcgcattc gggcttaaac tggcgtttgg
     4021 cgatcatgtt cactgctttt ttgccgaacc aacgcactcc ccttgtatgt tgttaggcgt
     4081 ccatacagga ttacacgatc agatttctgt tcaggatatt ggtatcgaca accttaccgc
     4141 agcggatggc cttgcagttg gtcgcgcatc aggctttgtc gggcgggcaa tggagcgtct
     4201 gctggatggc ttctataccc ttagcgatca aaccatgtat gacatgcttg gctggctggc
     4261 gcaggaagaa ggtattcgtc ttgaaccttc ggcactggcg ggtatggccg gacctcagcg
     4321 cgtatgtgca tcagtaagtt accaacagat gcacggtttc agcgcagaac aactgcgtaa
     4381 taccactcat ctggtgtggg cgacgggagg tggaatggtg ccggaagaag agatgaatca
     4441 atatctggca aaaggccgtt aataacgttt caacgcagca tcgcaatcct ttccctgggt
     4501 gagcgatgct gccgatggcg cagacttaag atccccggtc ttacccgcta taaccccctc
     4561 cttttatcat cacccaacgc ctttcgctgt aaacggcggt ttcgcaaacc aaaccaaaac
     4621 cgtaagtaag ataaaagcaa tagccgccat acggaaaatc tcatttgcag aaatagaaag
     4681 tgactgctgg gttatttcat tattaatttc attaagaact cctgaaagcg aaccataata
     4741 tttatccata atttgtgacg atgaattaaa cacggggtta aattgatcga tggttgctgt
     4801 caactgacta tggtgtaacg attcgcgtcg tccccacagc gtcattgtca acgacgtacc
     4861 aactgatcct gacaaggtac gaaaaaaatt actcatactc gaggcattgg caaatttatt
     4921 atctggcaag cctgaaaacg aaatcgttgt taagggtaaa aagaaacagg caacggcgaa
     4981 tccctgaaaa aactgcggca aaatgatgcc tgtaaaatca atcgttggca taaatgtcac
     5041 agaacgccag taatagcaaa ccgcatacat caaaaaacta aatgtcacta acaaccgcat
     5101 gtctattttg ttgccataac gtcctatcaa aggtgaaatt aatagtggca tgatgccgat
     5161 gggcgcataa gcaagtccgg cccatatcgc attatacccc atcgtttcct ggagtaactg
     5221 cggcataagg acgatcgctc cagagtaaaa taaatacgcg catgtgatac tcacaatacc
     5281 aatggtgaag ttacgggact taaacaaact gagatcaaga atcgggttct ctgaggtcga
     5341 ctcccaaatg actaaagaga tcagagaaat aactgatact actgttaata ttattattgt
     5401 actcgagttg aaccaatcca gatcgcgccc tttatcaagc ataatttgca agccaccaac
     5461 accgagcact aacagggtca gtcctggtag attcattttg accggtgaag tctcagtttc
     5521 tcttccttta agtaaggtta agcataatgt caggacgata atccccatag ggacattgat
     5581 taaaaatatc caaccccagc taaagttatc acaaatataa ccgcccaata tcggcccaca
     5641 tatcggagcg ataatcacgg tcattgacca taatgccaga gcaaatgttc ttttttctgg
     5701 cggataattc cttaataata aactctgtga cagtggaatt aacggccccg ccattaaccc
     5761 ctgaacgact ctaaaaaata tcagcacatc aagattggtc gataggctac acattaatga
     5821 agacagcgaa aaaaaggtta ctgaaagtaa aaataatctt aattcgccta ttctttgtgc
     5881 caacctgcca gtaacaggga tcgcaatggc atttgctaca ccaaacgagg tgataaccca
     5941 ggtgccttcg tctgttgatg ctcccagaaa gccagatatt gtcggtattg cgacgttaga
     6001 aatagtggaa tccaacattt gcataaatgt cgctaatgac aatgcaatag tgacgcacca
     6061 taacgtccca ccggttaatg gtgccggagt tgatttagtg attgccatta aagttgtcca
     6121 ttatgcgaaa taatgttgct aatttctttt tctatcggac tggtatcgat aactaaagcc
     6181 ttactggtat aagccggcat ggaggtcacg gttgaagcca gctcaggcat ctcagcaatg
     6241 tcttcgtcct tcgtatcaat agttgcagtc atcgataaac caatgcgcaa ggggtgttcc
     6301 atgagttctt ttggatcaag agaaacttca accggtacac gctgaacgat tttgatccag
     6361 ttccctgtcg cattttgtgc aggtaataag gagaacgcat tgccggttcc catattgatc
     6421 cctgtcaccc gaccatgaaa cacaacattt tcaccataaa gatcgctgat aatattgact
     6481 gattgaccaa tccgtacatc cgtgagttgt gtttctttaa agttggcatt aacccacatt
     6541 tgacgtgccg gtacgacagc cattaacgat tgtccgggac tcactgtttc gccgacctga
     6601 acacttcgct gggcaatata gccagtaacc ggactcctta tatccgtacg tttaagcgcc
     6661 aaccaggctt ctttggttgc atccgccgct tcaactactt gtggctgacg gtttaatggt
     6721 gtgttcatta ctaaagcttt attcgcttta taagcctgga tagcggcatt cagtgccgct
     6781 ttgctactta ttaacgtatc tttggtatgc tccagcgttt cttttgaaat aaccccctgc
     6841 ttcgctaaag gcacacgacg gttataatct tctaaggatt gttgatactg aatacgtgct
     6901 gaagcgactt cggcactgta ttgtttatcc tgtaagtaca gtttattcgt ttgccgaaca
     6961 atatttgcca gattattttt agctttattg agtgcgatag tggcatcagt tttatccagt
     7021 gaaactaaaa tgtcaccctg tcgaacgtag ttcgtatctt tatgattaac tacagtgaca
     7081 ctacctgaga cttgtgcaga aattggatct gcattccccg tgacataggc gtcatctgta
     7141 ctaatcatgt cttctaattc cattgaccaa taggcatagg cacctgaaaa cgcaataaat
     7201 aaaactatca ccaataaaga aaagtatttt tttctgttag actgtttttt atttgaatta
     7261 atctgttcca ctattatctc tcatttcgca tagatgaaat ttatgaattg aatgaaaaga
     7321 gtattcattc ttcatatcaa acacagtgca tgtgttgcac attatcccag gaacgttgga
     7381 ggcctaattt aaatgaggtg caaaaaaaac aacagaggac taaaccgtgg cttttgcaat
     7441 acaattctta cgcctgtagg attagtgaga agacttatag tgctaacttg aaactataaa
     7501 tcatcggtat aatccctgat tttattgttg acatttcatt tatgccgact atttatatgg
     7561 tatacttgtc gaattatctt aaaggaagct cagattttct tatttttatt gagaaaatga
     7621 gatgacgcct tatgtctgta ttactacagg gagaagggag atgcttcatt gcaaagggaa
     7681 taatctatga acgcaataat tattgatgac catcctcttg ctatcgcagc aattcgtaat
     7741 ttattgatca aaaacgatat tgaaatctta gcggagttaa ctgaaggcgg aagcgccgtt
     7801 cagcgggtgg aaacacttaa gcctgatatc gtcatcattg atgtcgatat ccccggagtt
     7861 aacggtatcc tggtgttaga aacgctgagg aagcgccaat atagcggaat tattattatc
     7921 gtctccgcta aaaatgacca tttttacggg aaacattgtg ctgatgctgg cgctaatggt
     7981 ttcgtgagta aaaaagaagg catgaacaat atcattgcgg ctattgaagc tgcaaaaaat
     8041 ggctactgct atttcccctt ctctctcaac cggtttgttg gaagtttaac gtccgaccag
     8101 caaaaactcg actccttatc gaaacaagaa attagtgtca tgcggtatat tcttgatggc
     8161 aaggataata atgacattgc ggaaaaaatg ttcatcagca acaaaactgt cagcacttat
     8221 aaaagtcgcc tgatggaaaa attagaatgt aaatcactga tggatcttta cacattcgca
     8281 caacgtaaca aaatcggcta accacatgaa gtttttaccc tatatttttc ttctctgttg
     8341 tggtctttgg tcgaccataa gtttcgcaga cgaagattac atcgaatatc gtggcatcag
     8401 tagtaacaac cgtgtcatac ttgatccagt acgtctgagc aacaaggaat tacgttggtt
     8461 agcgagcaaa aaaaatcttg tgattgcagt acataagtcc caaacggcta cgttgttgca
     8521 taccgattcg cagcaacgag ttcgtggtat taatgctgat tatttaaatc ttttaaaaag
     8581 aacgttaaat atcaaattaa cactccggga atacgccgat catcaaaaag caatggacgc
     8641 gctggaagaa ggtgaagtcg atatagtgtt atcacattta gtagcttcgc cgcctcttaa
     8701 tgatgacatt gctgcaacca aaccactgat aattaccttt ccggcgctgg taaccaccct
     8761 tcacgattca atgcgaccgc ttacctcatc aaaaccagta aatattgctc gagtagcaaa
     8821 ttatccccct gacgaggtaa ttcatcaatc atttccaaaa gcaacaatta tctcttttac
     8881 aaatttatat caggcattag catccgtctc agccggacag aatgattact ttattggtag
     8941 taacatcatt accagcagta tgatttcccg ctatttcact cactccttaa atgtagtgaa
     9001 atattataac tcaccgcgtc aatataattt tttcttgacc agaaaagaat ctgtcgttct
     9061 taatgaagta ctcaatcgat ttgttgatgc tttgacaaat gaagttcgct atgaagtatc
     9121 acaaaattgg cttgatacag gaaacctggc ctttctgaac aaaccattag aactcactga
     9181 acatgaaaaa caatggatta agcagcatcc cgatttaaag gtgctggaaa atccttactc
     9241 ccctccctat tctatgacgg atgaaaatgg ctcggttcgg ggcgttatgg gggatattct
     9301 taatattatt actttgcaaa caggtttaaa tttttctccg atcaccgttt cacacaatat
     9361 ccatgctgga acacagctga atcccggcgg atgggatata ttacccggtg ctatttatag
     9421 tgaagataga gaaaataatg ttttatttgc tgaagccttc ataacaacgc cttacgtttt
     9481 tgtcatgcaa aaagcgcctg acagtgaaca aacattaaaa aaaggaatga aagttgccat
     9541 tccatattat tatgagcttc attcgcaatt aaaagagatg tatccggagg tagagtggat
     9601 aaaagtcgat aacgccagcg ctgcatttca caaggtcaag gaaggcgaac ttgatgctct
     9661 ggtcgcgaca cagttaaatt cacgttacat gatcgaccat tactatccta atgaacttta
     9721 tcattttctt attcccggcg ttccgaatgc atcgctttcg ttcgcttttc ctcgcggaga
     9781 accggaactt aaggatatta ttaataaagc actgaatgca atccccccaa gcgaagttct
     9841 gcgcctgacc gaaaaatgga ttaaaatgcc caatgtgacc attgacacat gggacctcta
     9901 tagcgagcaa ttttatattg ttacgacatt atccgtttta ttagttggca gtagcctttt
     9961 atggggattt tacctgttac gctcagttcg tcgtcgtaaa gtcattcagg gtgatttaga
    10021 aaaccaaata tcattccgaa aagcactctc ggattcctta ccgaatccaa cttatgttgt
    10081 aaactggcaa ggtaatgtca ttagtcataa tagtgctttt gaacattatt tcactgcgga
    10141 ttactacaaa aatgcaatgt taccattaga aaacagtgac tcccccttta aagatgtttt
    10201 ttctaatgcg catgaagtca cagcagaaac gaaagaaaat cgaacaatat acacacaggt
    10261 ttttgaaatt gataatggca tcgagaaaag atgcattaat cactggcata cattatgcaa
    10321 tctgccagcc agcgatcatg ctgtttatat ttgtggttgg caagatatta cagagacgcg
    10381 tgatttaatt catgcactcg aagtagagag aaataaagcg atcaatgcaa ctgtcgcaaa
    10441 aagtcagttt ctggcaacaa tgagtcacga aataagaaca ccaataagct ccattatggg
    10501 cttcctggaa cttctgtcgg gttctggtct tagcaaggag caacgggtgg aggcgatttc
    10561 acttgcctac tccaccggac aatcactcct cggcttaatt ggtgaaatcc ttgatgtcga
    10621 caaaattgaa tcgggtaact atcaacttca accacaatgg gtcgatatcc ctactttagt
    10681 ccagaacact tgtcactctt tcggtgcgat tgctgcaagc aaatcgatcg cattaatttg
    10741 cagcagtaca tttcctgatc attacctggt taagatcgac cctcaggcgt ttaagcaggt
    10801 cttatcaaat ttactgagca atgctctcaa atttaccacc gagggggcag taaaaattac
    10861 gacctccctg gttcacattg atgacaacca cgctgttatc aaaatgacga ttatggattc
    10921 tggaagtgga ttatcgcagg aagaacaaca acaactcttt aaacgctaca gccaaacaag
    10981 tgcaggtcgc cagcaaacag gatctggctt aggcttaatg atctgcaaag aattaattaa
    11041 aaatatgcag ggcgatttgt cattagaaag tcatccaggc ataggaacaa catttacgat
    11101 cacaatcccg gtagaaatta gccagcaagt ggcgactgtc gaggcaaaag cagaacaccc
    11161 catcacacta cctgaaaagt tgagcatatt aatcgcggat gatcatccga ccaacaggct
    11221 attactcaaa cgccagctaa atctattagg atatgatgtt gatgaagcca ctgatggtgt
    11281 gcaagcgcta cacaaagtca gtatgcaaca ttatgatctg cttattactg acgttaatat
    11341 gccgaatatg gatggttttg agttgactcg caaactccgt gagcaaaatt cttccttacc
    11401 catctggggg cttacagcca acgcacaggc taacgaacgt gaaaaagggt taagttgcgg
    11461 catgaactta tgtttgttca aaccgttgac cctggatgta ctgaaaacac atttaagtca
    11521 gttacaccag gttgcgcata ttgcacctca gtatcgccac cttgatatcg aagccctgaa
    11581 aaataatacg gcgaacgatc tacaactgat gcaggagatt ctcatgactt tccagcatga
    11641 aacgcataaa gatctacccg ctgcgtttca agcactagaa gctggcgata acagaacttt
    11701 ccatcagtgt attcatcgca tccacggtgc ggctaacatc ctgaatttgc aaaagttgat
    11761 taatattagc catcagttag aaataacacc tgtttcagat gacagtaagc ctgaaattct
    11821 tcagttgctg aactctgtaa aagaacacat tgcagagctg gaccaggaga ttgctgtttt
    11881 ctgtcagaaa aatgactaaa tagcggctcc cacaatgttc taatgtggga gctatttacc
    11941 agcacatctt acatttatga tgagaactcc tggcgaattt gttccccatg ctggtcgagc
    12001 gttgccgctc ccggcataac atgcgggtcc gcgcagccgc tgatttttat cggattaccc
    12061 ggcatcatta ttcctccggc ttcaatcaac atatttctcg cctgagtttg tggcaaatta
    12121 atggcctcag ccacacttaa taacggcgcg acgggcacac caacttcatg tattttggct
    12181 aaccaaactt cagctgcctg cgtttttaac gttcgctcaa tatattgttt aagaatagcc
    12241 tggttttgta cgcgtaaaat attgctgcta aatcggggat cattaaccag ttccgtaagc
    12301 tccagtgcct ggcataacgc agaaaaaagc ttgtcattac cacaacaaat cgtaatcggc
    12361 ttatcctgag tatcgaaaac atcaaaaggt gccatgtagg gatggcgatt tcccaggcgt
    12421 tgtggtgact tccctgtcgc aatatatgcc atcagaccat gctccagaaa actcagcgtg
    12481 gcatcaaaca tcgctatatc gacatgcgcc cctctctggc tcttttcgcg gccataaagt
    12541 gcactcacta ttccgctgaa taaatagaca ccgccgcata gatccgcaag agaggtacca
    12601 acacgcactg gcggagcatc agggtaccct gtttccatca ttatcccact cattgcctga
    12661 atgatggtat cgtaggcagg agcatctttt agcggaccgg tatgtccgaa acctgacgat
    12721 gaagcatata tgaggcgcgg gttgatttct tgtaaccttt cccatgaaaa ccccagtttt
    12781 tccattgtac ctgggcgaaa attctcagct aatacatcag cttgtttgag catatttata
    12841 aatatacttt tatcgtgatc attctttaaa tcaagaacca cactctcttt gccatgatta
    12901 ataaaactgt aatagagtga ctgtccatcc acatagggac caaatgtgcg ggtatcatca
    12961 ccatgacctg gtggctcaac tttaattacc cttgcgccca tattacaaag aagttgagtt
    13021 ccgaaaggtc cattaaggac atgtgtcata tcgataacta ataagccttc aaacggccct
    13081 ttgctttcat tatttgtcat ttgcttatgc cttgaaaaaa gccgaaggtg aaaagacact
    13141 tcggcattta tgtaaaatca ataacatgat taattatgaa accagtcgac tgacataaat
    13201 ccacaaggga gccgtgacga caaagcccag tacgctcaca gccaatgacg ctgtaccagt
    13261 acgggtgtag acattaaacc ggctggcaat aatgattccg gagaatgccg gcggtaatgc
    13321 gcctgccagt accatcatct gcaaatgttc gctgttcaaa tgacatgcca taccaacgag
    13381 aagcagcgcc agtggcatca gaatcaactt caggaaggtg ttataagcaa tttcggcact
    13441 gaactcgaat ttatgtgctg ccagagtcaa ccccgcagcg aataccgcta cccctgagtt
    13501 agccttcgca atcagattaa aggtcgggtc ccatgctgcc ggaattttta ccccaaccag
    13561 caccaggatc gttgccagaa caggtgccca tactactggc tcctttgccg cagaaattaa
    13621 tgcgctcaga ttactattct tcttaccatc cgctcctgag gaaggattca gcaaatacag
    13681 accaatagga atagtaattg cgttaacaat aatagaaata attgctacca ctaaacctgt
    13741 tgatacggaa tcaccataaa taggatcgag aactgcaaac cccaggaatc caatggtagg
    13801 tgaacccgca attaatgcac atacagcggc ttctgcatgg gtacgtttaa aaaatttgta
    13861 gcaaccaaac caagagaaaa agaaacatcc gacaataacc acaagtgata caagggtcag
    13921 acgagtgtcc gcaaaaatca tttccctgtt tgcccgggta atagaaacaa atagagccgc
    13981 aggaagagcg tagtttaata ccagtttatt aaatgcgcgg gcttgatctt ctgaaaaagt
    14041 ttctcgtctg ccgctaaaat aacccaacag catgattacg ataataggca ataaatcgcc
    14101 aataaaaaat gttagcataa tagtatttcc gataagggtc ccctcaccat cgcataatga
    14161 gttggcaaga ggagacgatg tcagtattta attaccagcg acttgttttg ggttaagttt
    14221 ggtaatatgg ccactttcag ttcctgcagc agggtcgatg accacattaa taatggtcgg
    14281 tttgcgcgac tgaataccgg tggttaaagc atgacgaagt tcatctgtcg tggtgacgtt
    14341 atagccaacg ccacgaaacg catccattaa tttgtcatac cttgcatggt gcaacagatc
    14401 cgttggtgat ggtgcaccag cgccactgag atcaacaccg tctcctctgt agatgccgcc
    14461 attattaaaa ataacgatcg tcaccggcag gttatatcga caaatcgttt caatttccat
    14521 cccactgaaa ccaaaagcac tatcaccttc aatggcgaca accggagaac cagaagtcac
    14581 gctagcaccg atggcatagc ccataccgat gcccatgaca ccccaggtac cacaatccag
    14641 acgacgacgt ggtttataca tatcaataat atttcgtgca ttatccaggg tatttgcacc
    14701 ttcattaact aaataaatat cctggttctc gcgcaatacg tcgcgcacag cacttaatgc
    14761 attaaagtaa tttaatggtt gagtatctgt acttaatttt tcatgcattt tttgtgcatt
    14821 ttgctgcttg tggatattta aaatatcgcg ccataccagt ggagtcgtaa atgtgttttg
    14881 tttcagttct gccagcatac cttgcatact ggatgcaata tcaccaacga ctggcacagc
    14941 aatggggcgg ttgctgtcaa tttcctgcgg ttcaatatcc agttgaataa actgtgtatc
    15001 tgccgcccat ccttttttac cgtgtgccaa taaccaattc agtcgtgcac caacaagcat
    15061 gacaacgtca gcatttgcca gggcaaacga acgcgcagct gccgcagaaa gtggatgcgt
    15121 atcttcaagg atccctttcg ccatagacat tggcaggaat ggaatctgag cactttcaat
    15181 aaattcacga agctgttcat cagcttgtga atacgccgcg cctttgccaa ggataattaa
    15241 tggccgttca gcttttgcta aaagcgaaat tgcgctagtg actgacttcg ggcatggcaa
    15301 taatgctggc gacggatttt caactttaac aatcgtggtt aacgcttcgt ctttttccat
    15361 cgtcgcggcc aggacatttg ctggcaaatc aagataaact ccgccagggc gacccgatac
    15421 agagacccgg atagcgcgtg ccaatgcaat gccaagatcc tgcggctgat taacgcgaaa
    15481 tgctgctttg gcatacggtt ttgccgcatt catttggtcc agctcttcat aatcaccttg
    15541 ctgtaggtcg acgatcgcgc ggtcgctgga gccgctaatc atgatcatcg gaaaaccatt
    15601 taccgttgcg ttggccaatg cggtcaaacc attgaggaat cctggcgcag aaactgtcag
    15661 gcagatcccc ggtttttggg taagaaaacc gcttgccgca gcggcatagc ctgccgactg
    15721 ctcatgacga aaaccaatat aacgaatgcc ttccgcctgg gcatggcgtg ccatatccgt
    15781 cacaggaata cctacaacac cataaatagt gtcaatatta ttctgtttta atgcttcaac
    15841 gatgatatgc ataccatctg tcatttgaag ttgatctgac atgattaata acctcgaaat
    15901 ttttataaat tgggagccag aagcccccgt taatatcaga tggcgtggtt ttgcttcatt
    15961 gcagcaattt catcgtcgct ataacccagc tcctgcaata cagcagcggt atgttcacct
    16021 aatagcggcg cagctttaat atccggcgta aaggcagaga atttcattgg acaaccaacg
    16081 gtcagatatt ttccacgcaa cggttgttcc acttcaacaa cactgccact ttggcgcaaa
    16141 gagggatcaa gtgaaatttc tttcatactt aaaaccggtg cacaaggaat atcaaactga
    16201 gtcaaatagg ccaccgcttc atgtttatca atagtgacag tgtatttttc gatttcagca
    16261 aaaatatcga aaatatgtgg ctgtcgggca tgggctgtac tgtatgccga atcggtaatc
    16321 cattctggtt tgccgatggc tttacaggtg ttttcccagt tttgctcctg aatagtgaaa
    16381 taaatatagg cgttaggatc ggtttcccag cctttacatt tcaggatcca gccaggctga
    16441 ccgccaccac ctgcattacc accgcgggga actgcatcac caaatgtacc attcggatac
    16501 tgcgggtatt cttccagata acccaattta tcgagacgct gctggtcacg taatttcacg
    16561 cggcaaaggt tcaatacggc atcctgcatt gacatggtga ctcgttgccc acgccccgtt
    16621 ttttcgcgat gcagcaaagc agcaagtaaa ccgatcagca aatgcattcc ggtgttgctg
    16681 tcacccaacg ctgcagcgct taccagcggc ggaccatccc aaaaacccgt agtggatgcc
    16741 gcgccacccg ctgcctgagc aacgttttca taggctttta cattcacata aggcgaacac
    16801 tcatcaaacc ctttgatcga accaaaaatc agacgtggat tgatttcttg aatatgctcc
    16861 caggtgaagc ccatgtgatc aatggcccct ggatgaaagt tctcgactaa gatatcagct
    16921 tcgcggatca gcttttccat tacctctttg ccttccgctg ttttggtatt taactcaata
    16981 gaacgtttgt tactgttaag catggtgaag taaagcgcat cgatatcagg aatatctcgc
    17041 agttggtgac gcgttacgtc accaacgccg ggacgttcaa ttttaataac gtcagcgcca
    17101 aaccaggcca gcatttgagt acaagatggg ccagattgca caccggtgaa atcgagaact
    17161 ttaattcctt gaagtggagt tgacatagca atacctatta gttaattaaa tatgtgttaa
    17221 tttagcgttt atttaaggca taacgccctc tataaataat ctgcaaaata tcttcgtagc
    17281 ggcactcgat atttttaaat tcctgtatca agtactgaaa attatttcat cgccacttcc
    17341 aattcctttc tgcatggtaa gtagtgagga cgaaaagaag aatatgataa atatttattc
    17401 gtggtaatca gagagagtcg cgacatttgt gatgttattc ttacaactgt aagaagcgcc
    17461 ggtgatttac aagtaagagt atgattacgc tttttaaagc gtaccataaa gcataacata
    17521 ataaatgatt tgtgatgaca ctggattatt gcggtttact ttgaggaaaa taaaatgcgt
    17581 gtttatagaa ccgcgagcgc caaataattt tatgaccaca acctgtcgta ttgtggtcat
    17641 aaaaaattgt cggctggagg gagttaaatc ctttacctca atatactgcc tgtcagcatt
    17701 agttcacaaa cagtgcttcg ctatcgacga tgattccatc ttccgcacct ttaagcgcca
    17761 ggtttgcctc gtagtattgc tttttatcaa gcagtttctg cgcatcagca agtgcattac
    17821 gagtctgttt aagcggcatc agatattggt tttccattac gccaacaccg gccaggcgaa
    17881 gctcttccat tgcgcccttt ttatcccctt tcgccatttt ttcattggca atttttatcg
    17941 cggcttcttt ttcgggtgtt gctacataac tctcagaaat tccgacagat gcattaatga
    18001 caatatattg gtcatcattg agattggtct ttttacctgg cttagcgaac tttgcccatt
    18061 cggtactgtc atctgacagc aaagcggaag cttcattggt cagttctttt gccttttccg
    18121 gatcgccatg aaacagagcc aggcgcgcta cctgcacgtc acgcatcgca tataaacctt
    18181 gttcagagat acgctctgct gctgccgttt tctgggtttg ctgagtttgc tgttgtgccg
    18241 ctaccggtgc attatcagca gcccatacag ttgaagatgc cagaattgct gtgaccatcg
    18301 tagccataat taaacgtttc atacttacct cattctttaa cagttttaga aaacgccgcc
    18361 atcatattgg gtgagcatga aatgagaaat cagagagcaa cggtcatttt gaaagggtta
    18421 tcttacaatt gtagttatta tcgcaaagcg gcatttagtc ttacagttgt aggaatatgc
    18481 ttcctgtcgc agagaactat tcttatatac cacaaagaga tgtcatttat tatattgatg
    18541 taaatattga ttaattatat gtaaatacag ccataacatc gtatggttct cagtgaacca
    18601 ttttcaggat aactttctat gaaagtaaac ttaatacttt tcagcttatt tttattggtc
    18661 tctattatgg catgcaatgt ttttgcattt tccatttctg gtggtgtaag tgagaggagc
    18721 tataaagaga ctgaaaaaac atcagcgatg acaaccacac actctacaaa acttcagcca
    18781 tcacaggcga ttttgtttaa gatgagagaa gatgcgccac cattaaacct cacagaagaa
    18841 atcacgccta cttatccgac aaaggcgaat tatcttattc atcctgtgcg atagcgtctg
    18901 tcacatggca ggtgaacaat catgtctgat gctgatgtga acacctgccc aaatgagcca
    18961 tcctgaccca ctatcgcctg ccatacccca ccataaagcg aatcatcaag ccacaagccg
    19021 gggcaagaaa cataaatatc cccgtcacca gttcattacc gccagttaac cacatggtga
    19081 agtaataaat gagtgcagcg aggatcacca tgccgaagaa agaagagacc gccgatacca
    19141 cgttcagtac ctgaccgatg tagccgctta cgcagacaat acacagggcg agaaacatcc
    19201 ataaataaat catagctcaa cctcctgccg cgctaaaaaa cctgtaactt cagtcacttt
    19261 aatagtaaga caatccttaa cctctggcaa tttttgctaa gcatcaatga aaacagcacg
    19321 ttaaatgcaa cgctattgcg tgcggaaaat tttttacttt gcacgattaa taatcagtag
    19381 ctgaaagcag tcagccagtg ataaagtaga gccaccgcat tcatgctcct cgtggttatg
    19441 tcctgactag tctttacact ctttacagga accattgtcg tacatgatgg cccaaccaat
    19501 tgagtgtacc gctgtcgaca tggaatcttc tgcgatacaa caattcgtat ctacagaagg
    19561 taactatgtt tccacaatgc aaattttccc gcgagtttct acatcctcgc tactggctca
    19621 catggtttgg gcttggtgta ctctggcttt gggtacagct tccttatcct gttctctgct
    19681 ttctcggcac gcgtattggc gcaatggcgc gaccattcct gaaacgtcgt gaatctatcg
    19741 cccgtaaaaa cctggaactt tgtttcccgc agcattctgc ggaagaacgc gagaagatga
    19801 ttgccgaaaa ctttcgttca ctcggcatgg cgctggtaga aaccggcatg gcatggttct
    19861 ggcccgacag tcgcgtacgt aaatggtttg atgttgaagg gttggataac cctaaacgcg
    19921 cacaaatgca aaatcgcggc gtaatggttg tcggcgtcca ttttatgtcg ctggaactgg
    19981 gcggccgcgt gatgggactg tgccaaccaa tgatggctac ctatcgtcca cataataatc
    20041 agctgatgga atgggtgcag acccgtgggc gcatgcgctc taacaaagcg atgatcggca
    20101 gaaataatct gcgcggcatt gtcggtgcac tgaagaaagg tgaagcggta tggtttgctc
    20161 ccgatcagga ttatggtcgt aaaggcagct ccttcgcgcc gttctttgcg gtggaaaatg
    20221 tcgccacaac caatggcacc tatgttctct cccgtctctc tggcgcagcc atgttgaccg
    20281 taacgatggt aagaaaagcg gattacagcg gatatcgttt gtttatcacc ccagagatgg
    20341 aaggctaccc gacagatgaa aatcaagccg ctgcctatat gaacaagatt atcgagaaag
    20401 agatcatgcg cgcaccggag cagtacctct ggatccaccg tcgctttaaa acgcgcccgg
    20461 tgggagaatc gtcgttgtac atttaacaaa ctgctcttac tctttccctc ctcaaatcgg
    20521 ccataataac ctcatggttt ttatggcttt tttcttaagg ttttttcctt cgctgaatat
    20581 ctaattatta ccgtcagaaa ttaaactgtc gccgatatga gacacttgta agttactaaa
    20641 agtaaataag aaaatgcctc ttgtctcgtt tcagttttag gcgtacatta acgccgtctt
    20701 gagcagttta gctcagaatt ctggggtgat gcccacggta aaatacaggg cttttaaggt
    20761 cgctttgccc ggtgtcaact cactgtatca ggtgtaatga agtcattcag gcgtaacagt
    20821 aattacgcgg agagatgtaa agtgaaatat ttctttatgg gcatttcttt tatggtcatc
    20881 gtttgggccg gtacttttgc cctgatgatc taaaagcaaa ctgtgcaatg aaaaacagga
    20941 gccatcaggc tcctgtttgc atttctgacg acggtgttta ttccgcgttt tcgtgaatat
    21001 gtttgctgct ggcgggtagt aaaccgtcgg cacggaacat cgctttaatc cctctgatgg
    21061 cctgacgaat acggtcgcgg ttttcaatca gtgcaaagcg aacatgcgta tcaccatagt
    21121 cgccaaagcc aatccccggc gagacacaaa ccttcgcttc gttaaggagc ttcttggcaa
    21181 attccagcga tcccatggcc gcatatggtt ccgggatttt cgcccagaca tacatcgaag
    21241 ccttcggcat ttcgaccatc cagcccgctt catgcagccc tttaaccaat acatcacggc
    21301 ggcgtttgta ctgttcggta atgtcgcgca cgcactgttg atcgccctcg agtgccgcaa
    21361 tcgccgccac ctgcaacggc gtaaaagtgc cgtaatcgtg atagcttttg attcgcgcca
    21421 gggcactgac cagcgttttg ttgccgacca taaagccgat acgccagccc gccatgttgt
    21481 agcttttcga cagcgtaaag aactcgaccg ccacatcgcg tgcacccggc acctgcataa
    21541 ttgacggcgc tttccagcca tcgtagacga tatcggcata ggccaggtca tggaccacca
    21601 gcacatcgta gcgtttcgcc agcgccacaa ccttttcgaa gaactccagt tccacgcatt
    21661 gcgcggttgg gttcgacggg aagccgagga tcatcatctt cggtttcgga taactttcac
    21721 gaatggcgcg ctccagttcg ttgaagaaat cgacaccttc caccagcggc actgagcgta
    21781 cctgcgcccc ggcaatcacc gcgccataaa tatggatcgg gtaacttgga ttcggcacca
    21841 gcaccgtgtc accatgatcc agcgtcgcca gcatcagatg cgccaggccc tctttcgaac
    21901 caatagtgac gatggcttct gattccgggt cgatttcaac gtcgtagcga tcctgatacc
    21961 agcgggaaat ggcgcgacgt aaccgcggaa tgccgcgtga agtggagtaa ccatgcgtgt
    22021 ccgggcgctg ggccacagta cataattttt cgacgatatg cggcggagtc gcaccgtccg
    22081 ggttacccat gctgaaatcg ataatatctt cgccgcgccg acgcgcagcc attttcagtt
    22141 cagcggtgat gttaaaaacg tagggcggga gacgatcaat gcgcgtaaag cgacgttcag
    22201 ggcgagtgtc agccataatt tcctcagatt aacgttagcg cccggaccgt ccgagcgacg
    22261 ctgccacaat ggtggctgtt ttgaaaatag cctgattaat ttctgtctgt ccagcgtttt
    22321 agaacatttt tatccatggc tttaaatgag gaactccact tcaaaaacag gaaatttttc
    22381 tacgctaaca agaaaattac caccaatttt attaacatta taatttcaat aaatttacca
    22441 gccagaagtg cattatcaag agatacattg ctaatacacc atttatcttt caatcccctt
    22501 ttaaatttat ggtttttcct caaaaacggc ctgctggtca tcagccttca ggttacctat
    22561 catagaggtt taatccttat tcagagtcac ccgtgcacga aatattcaac atgctgctgg
    22621 cggtcttcga tcgggcagcg ttaatgctta tctgcctgtt ctttctcatc cgtatccgcc
    22681 tgtttcgcga actgttgcac aagtcggcgc actccccaaa agaattgctc gccgttaccg
    22741 ccattttctc gctgttcgcc ctgttcagca cctggtccgg cgttcccgta gaaggctcgc
    22801 tggtgaacgt acgtattatc gcggtgatgt ccgacgggat tctgtttggc ccgtgggtag
    22861 gcatcattac cggcgtgatt gcgggtattc accggtattt aattgatatc ggcggcgtga
    22921 cggcgatccc ctgctttatc accagcattc tggcgggttg tatatcaggc tggataaacc
    22981 tgaaaatccc caaagcacag cgctggcgcg tcagtattct cggcggcatg ttgtgtgaga
    23041 cgttgaccat gattctggta attgtctggg caccaactac cgcgttgggg atcgatatcg
    23101 tctctaaaat cggcattcca atgatcctcg gtagcgtctg tatcggcttt attgtgc
//
LOCUS       DAJTZL010000053        23120 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.053, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000053 DAJTZL010000000
VERSION     DAJTZL010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23120
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.053"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(268..885)
                     /gene="tdk"
                     /locus_tag="OO544_001797"
     CDS             complement(268..885)
                     /gene="tdk"
                     /locus_tag="OO544_001797"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HCR6767412.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            1490..1903
                     /gene="hns"
                     /locus_tag="OO544_001798"
     CDS             1490..1903
                     /gene="hns"
                     /locus_tag="OO544_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator H-NS"
                     /protein_id="HCR6767413.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(2047..2955)
                     /gene="galU"
                     /locus_tag="OO544_001799"
     CDS             complement(2047..2955)
                     /gene="galU"
                     /locus_tag="OO544_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HCR6767414.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(3157..4170)
                     /gene="rssB"
                     /locus_tag="OO544_001800"
     CDS             complement(3157..4170)
                     /gene="rssB"
                     /locus_tag="OO544_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HCR6767415.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(4262..5167)
                     /gene="rssA"
                     /locus_tag="OO544_001801"
     CDS             complement(4262..5167)
                     /gene="rssA"
                     /locus_tag="OO544_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HCR6767416.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            5280..5738
                     /locus_tag="OO544_001802"
     CDS             5280..5738
                     /locus_tag="OO544_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HCR6767417.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMAGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            5788..6630
                     /gene="purU"
                     /locus_tag="OO544_001803"
     CDS             5788..6630
                     /gene="purU"
                     /locus_tag="OO544_001803"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HCR6767418.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMAEAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            6790..6874
                     /locus_tag="OO544_001804"
     tRNA            6790..6874
                     /locus_tag="OO544_001804"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6824..6826,aa:Tyr,seq:gta)
     gene            6922..7053
                     /locus_tag="OO544_001805"
     ncRNA           6922..7053
                     /ncRNA_class="other"
                     /locus_tag="OO544_001805"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(7237..7914)
                     /gene="narI"
                     /locus_tag="OO544_001806"
     CDS             complement(7237..7914)
                     /gene="narI"
                     /locus_tag="OO544_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HCR6767419.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(7914..8624)
                     /gene="narJ"
                     /locus_tag="OO544_001807"
     CDS             complement(7914..8624)
                     /gene="narJ"
                     /locus_tag="OO544_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HCR6767420.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(8621..10159)
                     /gene="narH"
                     /locus_tag="OO544_001808"
     CDS             complement(8621..10159)
                     /gene="narH"
                     /locus_tag="OO544_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HCR6767421.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLEVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(10156..13899)
                     /locus_tag="OO544_001809"
     CDS             complement(10156..13899)
                     /locus_tag="OO544_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HCR6767422.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVSTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFLDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(14292..15683)
                     /gene="narK"
                     /locus_tag="OO544_001810"
     CDS             complement(14292..15683)
                     /gene="narK"
                     /locus_tag="OO544_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HCR6767423.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            16022..17818
                     /gene="narX"
                     /locus_tag="OO544_001811"
     CDS             16022..17818
                     /gene="narX"
                     /locus_tag="OO544_001811"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HCR6767424.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDEQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLLDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            17811..18461
                     /gene="narL"
                     /locus_tag="OO544_001812"
     CDS             17811..18461
                     /gene="narL"
                     /locus_tag="OO544_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HCR6767425.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(18462..19856)
                     /gene="ychO"
                     /locus_tag="OO544_001813"
     CDS             complement(18462..19856)
                     /gene="ychO"
                     /locus_tag="OO544_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HCR6767426.1"
                     /translation="MSRFVPRIIPFYLLLLAAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDDGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVRVEQYFGERVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            20041..20394
                     /gene="ychN"
                     /locus_tag="OO544_001814"
     CDS             20041..20394
                     /gene="ychN"
                     /locus_tag="OO544_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HCR6767427.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(20438..21133)
                     /gene="chaC"
                     /locus_tag="OO544_001815"
     CDS             complement(20438..21133)
                     /gene="chaC"
                     /locus_tag="OO544_001815"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HCR6767428.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPNE
                     GPVWIFGYGSLMWNPALEFTELCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(21291..21521)
                     /gene="chaB"
                     /locus_tag="OO544_001816"
     CDS             complement(21291..21521)
                     /gene="chaB"
                     /locus_tag="OO544_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HCR6767429.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            21791..22891
                     /gene="chaA"
                     /locus_tag="OO544_001817"
     CDS             21791..22891
                     /gene="chaA"
                     /locus_tag="OO544_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HCR6767430.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSVWHAIWLIIHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
BASE COUNT         5795 a         6052 c         5556 g         5717 t
ORIGIN      
        1 tctggggccg tttttttgtt tacccaaagc aacttttcca taaaccgaca gcattagcct
       61 tcatcatatt tgcgacgatg tataacgcct aaacacaggg atattgtact ttacaggtca
      121 caagtcaacg tcggtgctta agagccctgt gaggcgtata gcggcgttaa aaaactgccg
      181 agaagggtat atagcccgga agaagtgcgt aaaacgaact gacaggataa aagtgccctg
      241 ctcaccctgt cagtaaagaa attcttatta atcgtggcga tgcctttcct gaatagccgt
      301 taatgagccg acttgtaacg cctctttata gtgtttacgg catacagaaa cgtatcgttc
      361 attaccacca attaccacct gctcaccttc gttataaggt ctgcctgctt gatcaagacg
      421 cagcaccatg cttgctttac ggccacaaaa acagatggtt tttaattcaa ccagtttgtc
      481 ggaccatgcc agtaagtatt ggctgccaat aaataattca cctcgaaaat cggtacgtaa
      541 accataacaa agtacgggta tatcgagttg atcgacaacc tccgataatt catatacttg
      601 ttgtctggtt aaaaactggc attcatcaac cagtacgcaa tgaattgcct gctgttcatg
      661 ttccgcacga atctcatcaa ataatgatga attttggtta aataattttg caggcgatga
      721 caaacctata cgcgaactga ctttcccggc accaaagcga tcatcaattt ctgccgtata
      781 tacgacagtg cgcatgccgc gttcctggta attgtatgaa gattgcaaca atgctgtaga
      841 cttacccgca ttcattgcgg aatagtagaa atatagctgt gccatcagcc acaggccctc
      901 aatgatatga aataaagttg gctggagttt atcataattc gtcagttttt cagctcaaca
      961 gtatgcacag aagtgggtat atgcgttctc ccttacgaag ccttgcataa tccttctgag
     1021 ctatcattac aactgcctcg cttgttataa gcgggtaaat gactactggt aactattcac
     1081 aatctttaac ctgttgcgca agtaatagcc ctctgttgac ctccaggaga tagtgcaata
     1141 ctaagtccat gctcttattg cgactgttct acttttcatc attcgcttaa tagggaattc
     1201 tcgtaaacac aactaataca gaagactgaa aggtcgtcag cctacgataa tctccccata
     1261 aaatgtgaca tgaatcagga agttttaacc tcacgtgctg cgaaatcatc ggtgtaaata
     1321 gggctatatg ccgcgtcttt tctggctaat tttatgaaaa gatatttatt ggcggcacaa
     1381 aataaagaac aattttgaat tccttacatt cctggctatt gcacaactga atttaaggct
     1441 ctattattac ctcaacaaac caccccaata taagtttgag attactacaa tgagcgaagc
     1501 acttaaaatt ctgaacaaca tccgtactct tcgtgcgcag gcaagagaat gtacacttga
     1561 aacgctggaa gaaatgctgg aaaaattaga agttgttgtt aacgaacgtc gcgaagaaga
     1621 aagcgcggct gctgctgaag ttgaagagcg cactcgtaag ctgcagcaat atcgcgaaat
     1681 gctgatcgct gacggtattg acccgaacga actgctgaat agccttgctg ccgttaaatc
     1741 tggcaccaaa gctaaacgtg ctcagcgtcc ggcaaaatat agctacgttg acgaaaacgg
     1801 cgaaactaaa acctggactg gccaaggccg tactccagct gtaatcaaaa aagcaatgga
     1861 tgagcaaggt aaatccctcg acgatttcct gatcaagcaa taatcttttg tagattgcac
     1921 ttgcttaaaa tcccgccagc ggcgggattt tttattgtcc ggtttaagac aatttaataa
     1981 gaataagcta taaaaaaacg gcgtcgattg ctcaacgccg tttcgtggat aacaccgata
     2041 cggatgttac ttcttaatgc ccatctcttc ttcaagccag gctttaaatt ccgtgccaag
     2101 ggtgttatga cgaataccgt attcaacgaa ggcctgcatg taacctaatt tattaccgca
     2161 gtcatggctc ttccctttca tatgataggc ttccaccgtt tctttttcga tcagcatatc
     2221 aattgcgtcg gtgagctgaa tttcatcacc agctcccgga ggggtttttg ccagcaacgg
     2281 ccaaatatcc gcgctaagta cgtaacgacc cacaatagcg agattagacg gcgcaacatc
     2341 cgctttcggt ttttctacca caccaaccat cggtacgctt tcacccggcg ctaattcaac
     2401 gcctttgcaa tccacaacgc catatgcggt cacatcagca accggttcaa ccatgatctg
     2461 gctatgaccc gtttcatcaa agcggcggat catctctgcc aggttatcct gtgacaaatc
     2521 ggattcatat tcatccagaa taacatcagg caaaataaca gctaccggtt catcacccac
     2581 taccgggtga gcacacaata ccgcgtgtcc caggcctttc gccagaccct gacgaacttg
     2641 cataatagtc acgtgcggtg gacaaataga ctgcacttca tcaagcagtt gacgttttac
     2701 acgtttttcc agcattgctt ccagttcaaa actggtatca aagtggtttt caatagagtt
     2761 tttagatgag tgtgtaacca gcacaatttc agtaatgcca gccgcaatac attcattcac
     2821 gacgtattga attaatggct tatcgacaag tggcagcatc tctttcggga tggctttcgt
     2881 cgccggcaac atcctggttc ctaatcccgc aacggggata acggcttttt tgactttcgt
     2941 attaatggca gccatttaaa ttctcctgga ctgttcgtgt tttgaacgtg ttcatatttc
     3001 tgtatcgcat cccagtatat cagcaccccg aaggggctaa gttctgaaaa ggaggcgagt
     3061 tagcataatt aagacaatta cttatacgaa tggcgacgat agtaccacca gccgacatta
     3121 gcaggtaatg caaatttagc ccgcgttatc gtttgctcat tctgcagaca acatcaagcg
     3181 cagtcgacca ccggttcccc atatttggca ttgccaggca tcgcatcgct ggctcaattg
     3241 attcaaataa gcgttaccta aagtgcctaa cggaacacca ttactgattt gcacctggtg
     3301 ttcgccggta tttaacgtcg cattcagacc cgcagaaacc agaatgagat ttttcagttc
     3361 gcgatgataa tagccaacta atagcggaaa ctgccccggc agattggcct gacggagtaa
     3421 atggtttacc tgcttcaata acgcgcccaa ctctggcaac cgttgatttt ggtgtgcaag
     3481 ctgttcctgt aataatccgt taaacaatgc gcgtaataac aaggcagcaa gtacgccatt
     3541 atgtccagct cgggtgacat caaggcaata aaatgccaga tcgttttccg aaagtgcggc
     3601 aatatcaagc accaggccgg gtttgtccgc ggcaaccaat tgacgataat taacccggca
     3661 atgggaaatc acctgctgaa ccggcggttg tagttcctgt aataatttcg ccgctgcggc
     3721 agggttatca accattgcat cccagtcgcg aaaaagcctt tcctcttcct caacgcgcga
     3781 attaaacatg ctgggataga gacaggcaaa aaccatctcg cgcaagcgat tcagatcttt
     3841 aactggtttc agcaaaacat cttcaacgcc cagacgtaac gctttggcaa tatctgccat
     3901 attttcagtg gcagatatca ccagaactgg ggtctggtcg cctctgttac gtatatgctc
     3961 cagcagttta agcccgttca ttcgtggcat cgcgatatca catatcatca ggtctggagt
     4021 gaaacctccc agcaactcaa gggcatccac cccatcagcc gccagtaccg ttgtcgctcc
     4081 caatgaggaa aaccatgaat ccagaagcga gcgaaatacc tgctcatctt caacaatgag
     4141 aatctgtttt ccgaccaatg gctgcgtcat gttctctccc ctgactggct ttactcaata
     4201 gtggcatgct attgccacct gcgcctgtca gaattaactt aagtgtagat aaaaattctg
     4261 gtcaaatgtt ggtgcgtacc aacggcaaaa gttcgtccat tttcctttcc actgccagct
     4321 gtccggccgc aatggcagcg tgcgcgcgat ggaaatcaag cgtagatatt tgcgggcaaa
     4381 caggttgaat cagaatatcg ggcggatcac ctgccatgcg gttcctttta aggcggttct
     4441 ccaacacctg gatagacgtg gtcataatct ctgttgccgt tggcgctgtc acagcccgac
     4501 gtgtcgttat gctgcctaac ctttctttca gacgcgcatg ccacggtaga gaatcaccat
     4561 tctcgctatt ttcttcactg acattaaagg agagcagatc ttgttgcatc aaatgagcat
     4621 cgtgctgcag gtcgaccgct atcacaatat cagcccccaa tgcacgcgta agagaaatag
     4681 gaattgggtt aactactgcc ccatcaacca gccagtagcc gttatgtgca acgggtgcca
     4741 tgagtcctgg aatactgcat gatgcgcgaa tagcaagatg gagatcgcct tcagtaaacc
     4801 ataattcacg ccccgtactt aaattggtgg caacagccgc aaagcgacgg gaacaatttt
     4861 cgatctctgt ttccggcatt atttcgcgat attgattgaa gacacgctcg ccgcgcagta
     4921 acccaccgcg ctgccaggag aggtccatca ggcgtaaaac gtcccaataa ctgaaagagg
     4981 ttacccaatc ttccagcgca gataatcgat cgcatgcata ggccgcgccc accagcgaac
     5041 caattgaaca tcctgcaacg atatcaattt caatacccac tttttttagc gcattaataa
     5101 cgccaatatg cgaccaacct ctcgccgcgc cagatcccag cgccagccct atttttatct
     5161 ttctcattat tcccgcaaga ttcccctgga atgcaatcgt agccacatcg ctactgctcg
     5221 gttaacatag tgctactcag gcggttaata cgccgttatt gtttccaggg agattatttg
     5281 tgtctcagct ttgtccctgt ggtagtgctg tcgagtatag cctatgttgc cacccttatg
     5341 tgtctggtga aaaggttgca cctgatccag aacatctcat gcgttcgcgt tactgcgctt
     5401 ttgtgatgca agacgcagat tatttaataa agacctggca tccctcttgt ggagcagcgg
     5461 cgttacgtgc cgaattgatg gccggatttg cgcatactga atggttggga ttgaccgtat
     5521 ttgaacattg ttggcaggac gccgacaata tcggatttgt cagttttgtt gctcgattta
     5581 ccgaaggtgg caaaacaggt gcaataattg aacgttcgcg atttttgaaa gaaaacggtc
     5641 aatggtacta tattgacggt acacgtccgc agtttggtcg caacgatccc tgcccttgtg
     5701 gttcaggtaa aaaatttaaa aagtgctgcg gccaataatg gttgacggta cggtttagca
     5761 aacactctca acaaggtttt tccagcaatg cattcactcc aacgtaaagt tctgcgtact
     5821 atttgtccgg accaaaaagg tctgatcgca cgtattacca atatttgcta caagcacgag
     5881 ttaaatatcg tacagaacaa tgaatttgtt gatcaccgta ccgggcgctt ttttatgcgc
     5941 acggaactgg aagggatttt taatgattcc accctgctgg cggatctcga tagcgcattg
     6001 ccagaaggct ccgtgcgtga gctgaatcct gccggtcgtc gccggatagt gattctggtc
     6061 actaaagaag cgcattgcct tggcgatttg ttgatgaaag ccaattacgg cggcctggat
     6121 gtcgaaatcg cggcagttat tggtaaccac gatactttac gttctctggt tgagcgtttt
     6181 gatattccgt ttgagctggt aagccatgaa gggttaagcc gcaacgagca cgatcaaaag
     6241 atggcggaag ccattgatgc ttatcaacct gactatgtgg tgctggcgaa gtatatgcgg
     6301 gtattaacgc cggaatttgt ggcacgcttc ccgaataaaa tcatcaatat tcaccattca
     6361 ttcctgcctg cgtttatcgg cgcacgtcct tatcaccagg cctatgaacg tggtgtgaag
     6421 attattggcg caaccgctca ctatgtgaat gacaatctgg acgaaggccc aatcatcatg
     6481 caggacgtta ttcatgtcga tcatacctac acagctgaag atatgatgcg cgcaggtcgt
     6541 gacgtcgaga aaaacgtctt aagtcgtgcg ctatacaaag tactggcaca gcgcgtcttt
     6601 gtttacggta atcggacgat tattctttaa tcgccagcaa aaataactgg ttacctttaa
     6661 tccgttacgg atgaaaatta cgcaaccagt tcatttttct caacgtaaca ctttacagcg
     6721 gcgcgtcatt tgatatgatg cgccccgctt cccgataagg gagcaggcca gtaaaaagca
     6781 ttaccccgtg gtggggttcc cgagcggcca aagggagcag actgtaaatc tgccgtcatc
     6841 gacttcgaag gttcgaatcc ttcccccacc accatcactt ttcaaaagtc cgtaaactct
     6901 caaacgaatc cgcaattaaa tattctgccc aagcggggaa ggatgagaag cttcgaccaa
     6961 ggttcgactt gagcgccagc gagagagcgt tgccgcaggc aacgacccga agggcgaagc
     7021 gcgcagcgct gagtaatcct tcccccacca ccatcgcttc cttaaataat caaaacctgt
     7081 tactccgagc taattttgcc cgatggcacc acgtttaccg ggcctacaat atcctcccct
     7141 gtaggtcgga taagcgaagc gcatccgaca actcaccccg caccgtgccc ataaaaaaac
     7201 cccgccgaag cagggtctat gtgaactaaa attcgcttag tgacgagcgc gcaccagctg
     7261 gtacttacgt gtcagatact ccaccggtac gctccagatg tgtaccagac gcgagaacgg
     7321 gaacagcagg aataacgtca tccccagcac caggtgcaga cggaagataa acgccacacc
     7381 atcgaggtgt tgagaagcgc caccgtggaa ggtcaccacc gactgcgccc agccaaccag
     7441 tttcatcatc tcgctaccgt ccatatgctg agcggagaac ggaatggtca acaggcccag
     7501 cgcgcactgg ataacgagca gcgacaggat caggatatcc gctccggtag tggttgcacg
     7561 cacgcgtggg ctgaacagac gacgcttcag caacagcacg ccgccaatca gacacagcac
     7621 gccgctggca ccaccagcaa acatcgccat tttctgtttc acttcaatcg gcagccaggc
     7681 ttcatacatc cagtgcggcg tcagcatacc gaagaagtga ccaacaaaaa tccccagaat
     7741 accgatatgg aacaggttcg acgccaggtt catccctttg cgatccagca tctggctgga
     7801 cgccgcgcgc caggtgtact gcccgtagtc ataacgcagc cagctaccaa tcaggaagac
     7861 cgccccggct atgtacgggt agatatcaaa gaagaacata ttcaggaatt gcattagtgc
     7921 tgtcctccgg tggtgatatt cagatattgc ggcgcgacgg caccggcaaa gcgacgctga
     7981 tgagcagtga ttgctgaatc accgcagcct ttgtcagcaa agaatttaac ctgctcttct
     8041 tcccagacag catccagcgc ctgcggcgta tcatcgcgcg cttcgtcggc aattttttcc
     8101 gccactttgt cgctgtcgat agcggtattg gccaatttca gcagcagatc aaacatcacg
     8161 gcataacggc tttcacgctg ttgcagacgc gcgctcagca atgccagaat cggcgcgata
     8221 tctttcaaac cttccacggc ttcgctttgc ggcagctgcg acaggtactc cagatacagc
     8281 ggcagatggt ccggtaattc gcggctgttt aactgcaagc cgtgctgctc gtactgcgcc
     8341 agcaggtcca ccatcgcctg gccgcggtca cgggattcac cgtgaacatg ttcaaatagc
     8401 aacagtgacg tggcgcgacc acggtcgaac agttcgctgt actgggcctg ggcatcgagc
     8461 ggatccatcg ccgttaaatc gcgcaggaaa atgcccagcg catgggcatc ttctttcgac
     8521 agatttttcg acgcagcaat cgcctcaaac atctcttgtt gatgctgcca taaggcagca
     8581 tccggatatt caaggagacg cgatacaatc acgagttcga tcatggatgc ggctccgttt
     8641 tgctggtcac atcgatggca tcgatacgac ggctgttgaa cagattgaat ttggtatctg
     8701 aaccgtggca gccatcacca aaggtaaagc cgcagccatt tttctccggg aaggcttccc
     8761 gtgccagttc acgatgacta ctcggcacca caaagcgatc ttcgtagtta gcaatcgcca
     8821 gataacggta catctcctgt gcctgggctt cggtcagacc gacctcttcc agcgcacggg
     8881 tatcaacttt accgtcaacg gtttcagcac gtttgtaatg acgcatcgcc agcatacgtt
     8941 tcagtgcgcg cagtaccggt ttggtatcac cggcggtcag cagattcgcc agatactgta
     9001 ccggaatacg caagctttcg acgtctggca gaatgccgtt gctacccagc tcacccgcgt
     9061 ctgctgcaga ctgaatcgga gacagaggcg gcacgtacca gaccatcggc agtgtgcgat
     9121 attctggatg cagcggcagc gccagtttcc attccattgc cattttataa accggcgact
     9181 gctgtgcggc ttcaataacg ctcagcggaa taccgtcttt aatcgcctgc tcgatgactt
     9241 tcggatcgtt cggatcgagg aacacctcca gctgacgctg gtaaagatct ttctcgttct
     9301 cggtgctggc tgcacgttca atcgcgtcgg catcgtacag cagcacgcca agataacgga
     9361 tacgaccgac acaggtttct gagcacacgg tcggctgacc cgcttcaata cgcggatagc
     9421 agaagatgca cttctcagac ttaccgctct tccagttgaa gtagattttt ttgtacgggc
     9481 atccggtgat gcacatacgc cagccacggc atttatcctg gtcgatcagg acgatgccat
     9541 cttcttcacg cttgtaaatc gcaccgctcg ggcaggtcgc cacacatgcc gggttcaggc
     9601 agtgttcgca caggcgcggc aaatacatca taaaggtgtt ttcgaactgg ctatacatcg
     9661 ccttctggat gttgtcgaag ttcttgtctt tcgccagttt gtcaaactca ccacccaggt
     9721 catcttccca gttcggccct ttttcgattt tcgccatccg ttccccggta atcagcgaac
     9781 gcggacgggc aatcggctgc gatttgctgc cttccggcgc ggtatgcagg ttctgatagt
     9841 caaaatcgaa cggctcgtaa taatcgtcga tccccggcag atgcgggtta gcgaagattt
     9901 tacccagcaa catggcacgg ttacccatgc gcggctgcag tttgccgttg attttacgga
     9961 tccagccgcc tttgtatttt tcctggtttt cccagtcagt cgggaagccc tgacccggct
    10021 tggtttccac gttgttgaac cacgcgtatt ccacgccttc acggctggtc cagacgtttt
    10081 tacaggtaac tgaacaggtg tggcagccga tgcacttatc gagattcagc accatgccga
    10141 cttgtgaacg aattttcatt ttacgctctc ctgtacctgg tcattgcctt cgccatctaa
    10201 ccagtcaatg ttcttcatct tacgcactac aacaaactca tcgcggttag aacctacggt
    10261 gccgtagtag ttaaagccgt atgccagatg ggcatagccg ccgatcatat gcgtcggttt
    10321 cggcgtgata cgggtgaccg agttatggat accaccacgc tgttgggtaa tttccgaacc
    10381 aggcaggtta acgatacgtt cctgcgcgtg gtacatcatg gtcatccctg ccggaacacg
    10441 ctggctgaca accgcacggg cagtcagagc accgttgctg ttgaatactt caatccagtc
    10501 gttatcggcg atacccagat ctttggcatc ggcttcactc aaccagacca ccggaccacc
    10561 gcgacctaaa gtcagcatca gcaggttgtc gctgtaggtg gagtggatac cccacttctg
    10621 gtgcggcgtc aggaagttga gcgctttttc cgggttgccg ttggatttct ggcctatcac
    10681 ttctttcacc gaacgggtgt cgatcggcgg acgataaacc agcaggcttt caccgaaatc
    10741 acgcatccac tggtgatcct gatacagttg ctgacgacca gagagcgtac gccatgggat
    10801 cagctcgtga acgttggtgt aaccggcgtt gtaagaaacg tgttcatctt ccagaccaga
    10861 ccaggtcggg ctggagataa ttttgcgcgg ctgtgcctga atatcgagga agcggatctt
    10921 ctcgtcttct ttattcagcg ccagatgcgt atggtcacga ccggtaaatt cgctcagggc
    10981 agcccaggct ttcacggcta cctgaccgtt ggtttccggt gccagtgtca ggatcatctc
    11041 tgccgcatca attgcggtgt tcagcatcgg ctggcctttc gccggacctt ccgctttggt
    11101 gtagttgagc ttacgcagca gatccatctc gctctgggtg ttccaggcaa tccctttacc
    11161 gccattaccg attttctcca tcaacgggcc aatagaggta aagcgttcgt aagtcgccgg
    11221 ataatcgcgc tctacgacca taatgtgtgg cgcggtttta cctgggatca ggtcgcactc
    11281 gccttttttc cagtctttca catccagcgg ctgcgccagt tcagcggcag agtcatgctg
    11341 gataggcagc gtgacgatgt cggtttcttt acccagatgg ccgacgcaca cttcggagaa
    11401 tttcttcgcg atggctttgt agatttccca gtcgcttttc gcttcccagg ccggatcgac
    11461 cgccgcagac agcgggtgaa taaacggatg catatccgaa gtattcatgt cgtctttttc
    11521 gtaccaagtc gccgtcggca aaatgatgtc ggaatagaga caggtgctcg acagacggaa
    11581 gtccagcgta accaccagat ccagcttgcc ttccagacca ttgtcctgcc agtccacttc
    11641 ttccggcttc acgccgccct gttgccccag atctttaccc tggataccgt gctccgtccc
    11701 cagcaggtac ttgagcataa actcatgacc tttaccggaa gaaccgagca ggttagaacg
    11761 ccagatgaac aggttgcgcg ggtggttttt accgttttct ggttgttctg ccgcaaaacg
    11821 gatggaaccc tctttcaggg atttcaccgt atagtccacc ggattcatcc cggctttttc
    11881 cgcttcgcca gcgatagtca gcgggttagt gcctaactgc ggtgcagacg gcagccagcc
    11941 catgcgttcc gcacggacgt taaagtcgat caagtgtccg gtatagcggg atttgtccgc
    12001 catcggtgac agcaactctt ccgccgtgac ggtttcataa cgccactggc tggagtggtt
    12061 atagaaataa gaagtgctgt tcatgtgacg cgccggacgc tgccagtcaa gggcaaacgc
    12121 cagcggctgc cagccggttt gcggacgcag tttttcctga cctacatagt gcgcccagcc
    12181 gcccccgctc tgaccgacac agccgcagaa aatcagcatg ttgatcagac cacgatagtt
    12241 catatcgagg tgataccagt ggttcagccc cgcaccgacg ataatcatcg aacgaccgtg
    12301 cgttttatca gcgttatcgg caaattcacg ggcgatgcga ataatctggc tgcgagaaac
    12361 gccggtaatc tgctcggccc aggccggggt ataagctttc acatcgtcat agctggttgc
    12421 acagttaacg tcgttcaggc cacgttccag accgtagttt gccagcgtca gatcataaac
    12481 ggtgctcacc agggcggtgc tgccatcagc cagttgcagg cgtttcaccg gcagtttgtg
    12541 cagcagcacg ttttccagtt ccactttgtt gaagtgttcc gtgccgtcgc caccaaagta
    12601 cgggaagcct acctcagcga tctcatcctg gctacccagc aggctcagtt gcagctcggt
    12661 ttcttcgcca gttttgccgt cgcgctgctc aagattccat ttgcccttct cgccccagcg
    12721 gaagccaata gaaccgttcg gcgcaaccat ttcgccattg gtattaaagg cgacagtttt
    12781 ccattccgga ttgttttcct ggcccagcgc atcaaccaga tcagcagcgc gcagcatacg
    12841 acctgcagcg tagtaaccgt cgcgttcttc cagcatcacc agcatcggca tgtcggtgta
    12901 gcgacgcaca tagtcggtga aatactggct tgggttgtcg aggtggaatt cacgcagcat
    12961 tacgtggccc atcgccagcg ccattgccgc atcggtgccc tgtttcggtg ccagccacag
    13021 atcgcacagt ttggcgattt cagcgtagtc tggtgtgacg gcaacagttt tggtgccttt
    13081 gtaacgcact tcagtaaaga agtgagcatc cggggtacgc gtctgcggca cgtttgaacc
    13141 ccaggcgatg atgtagctgg agttatacca gtcagcagat tccggtacgt cagtttgctc
    13201 gccccaggtt tgcggagacg caggaggcag gtcgcagtac cagtcgtaga agcttaagca
    13261 agtaccgcca atcagcgaga gatagcgtgc acccgatgcg taagaaacca tcgacattgc
    13321 cggaattggc gagaaaccag caacacggtc cgggccgtag tttttgatgg tgtaaacgtt
    13381 agatgcggcg atcagttcgt tcacctcctg ccaggaagaa cgaacaaatc caccgcgtcc
    13441 acgcgcctgc ttaaagcttt tcgctttatc ggcgtcttca atgatagaag cccatgcctc
    13501 aaccggatcg ctatgcagcg ccttcgcttc acgccacatt ttcatcaggc gtttgcgcat
    13561 catcgggtat ttcaggcggt tggcactgta aagataccag gagtagctgg caccgcgcgg
    13621 gcagccgcga ggttcatggt ttggcagatc cggacgggta cgcggatagt cagtctgctg
    13681 ggtttcccag gtgaccagac cgtttttgac gtagattttc cagctgcagg agccggtgca
    13741 gtttaccccg tgggtagagc ggacgatttt gtcatgctgc caacgctggc gatatccatc
    13801 ctcccagtca cggttggtat tgagaagctg gccatgccca tcggcaaagg tttcaccctt
    13861 ctgtttgaag tagcgaaacc ggtccaggaa tttactcatc ggttttctcc tgtgggagcc
    13921 tgtcggccct ctgataaatc gacattgcta atgtttaagg gcaaggtaac gctctgaaac
    13981 agcgtgggaa ttgataacga tcaagagtga tggggatgaa aaataaagta aattcctaag
    14041 ataccccttt aggataatta acagccacca cataacagac tgtaaattaa gacgttttat
    14101 acctgtagtt atttaaacta ttaagatttt tatgcagatg ggtattaagg agtattcccc
    14161 atgggtaaca tattaatggc tcttaatgaa atccggcgaa ataagccata taatatgagt
    14221 atgttgtaac taaatgacgc ataaaaaaag cgcgacatca tgccgcgcaa aggataatca
    14281 aatattaaga tttatttttt agaatgccga ccatataccg cccaggtaat cactacgcag
    14341 gcgatataga aaatcaaaaa taccttcatt gcgccgactg gcgaacccgt taatgccagc
    14401 gagctaccaa acgctttcgg gataaagaag ccaccaatag cgccaatcgc agagatgaaa
    14461 cccagcgccg ctgccgtgtc ggttgccgct tcacgcatcg cacgttcgtc agaaccccct
    14521 tctgctttca cgcgatccat tgtcagttta cggaagatca ctgaaatcat ctggaaagtg
    14581 gaaccactac ccagcccagc tgtcaggaac agcgccagga agaccgcgaa gaacgccatg
    14641 aagcttccgc cctgcccgtc agtcggtaag gtcaggaaca gcaggccgct gaaaatcgcc
    14701 atcagaataa agttcaccag cgtgacacga gttccgccca gacggtcaga taatgcacca
    14761 cctgcagaac gcgccagcgc accaataaac ggcccgaaga aagcgtattg cagaatctga
    14821 acatccggga actgcgtttt tgacagcatc gcaaagcccg cggagaagcc gataaaggag
    14881 ccgaaggttg ccagatacag caggctcata atccacagat gaccccgttt gagtaccggc
    14941 aactgctcct tgatggaggc tttcgaggta gcaagatcgt tcatgccaaa ccacgccgca
    15001 atggtgaaga tggcaaggaa cggcacccat atccaggacg cattcgccag atacagctca
    15061 gtcccatccg gctgtttgac gccctggcta ccaaatactg cgaaaatcga cagtgatacc
    15121 accagcggag caaccaactg catgacgctg acgcccatgt ttcccagacc accattcaga
    15181 cccagcgcgc caccctgctt ctgtttcgga aagaagaagc tgatgtttgc catactggat
    15241 gcgaagttcg cgccagcaaa gccgcatagc agagagatga tgatgaagac gctataaggc
    15301 gtggaggtat cctgcacggc aaaacccagc cagacgcaag gaataatcag aataccggtg
    15361 ctgaacgccg tccagcgacg accaccgaag ataggaacca taaaggagta tggaacacgt
    15421 aataacgcgc cagaaaccga aggcagcgca gtcaacataa atagctgatc ggtcgtaaaa
    15481 ttaaaaccga ctttcggtaa gttcaccgca acagcgctga acaacatcca tacgcaaaac
    15541 gccagcagca gacagggaac ggaaatccac aggttacggc tggcaatacg ttgaccgcgt
    15601 tgttgccaga acgcaggatc ttccggtcgc caatctgtaa tgacagctcc agtagccctt
    15661 tcgggggcgg atgagtgact catagacacc tctgatactc gtttcgatgt ctgccacctt
    15721 agtgtctgta gctaaaggca atttgatgta aatcaaacga taagataact ttatctatca
    15781 ttgatattta tcattaccca tagtgagtac agtgatttca taaaaattat gagatttttc
    15841 acggtgctgt aaaatcccta cccttaccga tgtaaagcga ctaaccacac ggcaaataag
    15901 gagtaactct ttccgggtat gggtatactt cagccaatag ccgagaatac tgccattcca
    15961 gaatgtatcg tcacattcat taaggttatt gctcatttaa agcctgaagg aagaggtttg
    16021 catgcttaaa cgttgtctct ctccgctcac cctggttaat caggttgcgc ttattgtgtt
    16081 gctttctact gctattggac tggcagggat ggcggtttct ggctggctgg tgcaaggcgt
    16141 tcagggcagc gcccatgcga tcaacaaagc gggatcgctg cgcatgcaaa gttaccgtct
    16201 gttggcggca gtgccattaa gtgagaaaga caagccctta attaaagaga tggaacaaac
    16261 ggcatttagc gccgagttaa ctcgagcagc agaacgagac gaacaactgg cgcaattaca
    16321 gggtttacaa gattactggc gtaatgaact gatccctgca ctgatgcgtg cacaaaaccg
    16381 cgaaacggtg tcagcggatg tcagccagtt tgttgccggg cttgatcagc tggtatctgg
    16441 ttttgaccgc accacggaaa tgcgcattga gacagtggta ctggtccatc gggtaatggc
    16501 ggtatttatg gcacttttac tggtgttcac tattatctgg ttgcgggcgc gactgctaca
    16561 accgtggcgg caactgctgg caatggcgag tgccgtcagt catcgcgatt ttacccaacg
    16621 cgcgaacatc agcgggcgca acgaaatggc gatgcttgga actgcattga acaatatgtc
    16681 tgcagaactg gccgaaagtt atgccgtact tgagcagcgg gttcaggaga aaaccgccgg
    16741 gctggagcat aaaaatcaga tcctctcttt tttatggcag gctaaccgcc gtttgcattc
    16801 ccgcgccccg ctgtgtgaac gcctgtcacc tgtactcaac ggcttacaga atttaaccct
    16861 gctacgtgat atcgaattgc gggtgtatga cactgatgat gaagagaatc atcaggagtt
    16921 tacctgccag ccagatatga cttgtgatga taaaggctgc cagctctgcc cgcgcggcgt
    16981 attacccgtt ggtgatcgcg gcacgaccct gaagtggcgg ctggctgact ctcatacgca
    17041 gtacggtatt ttgctggcga ccctgccgca ggggcgtcat cttagccatg atcaacaaca
    17101 actgctggat accctggttg aacaactcac cgccacgctg gcgctggatc gccatcagga
    17161 acgtcagcaa cagttgatcg tgatggaaga gcgtgccacc attgcgcgcg aactgcatga
    17221 ttctattgcc caatctctct cttgcatgaa gatgcaggtg agttgtttac agatgcaggg
    17281 cgatgcgctg ccagaaagca gccgcgaact gttaagtcag atccgtaacg aactgaatgc
    17341 atcctgggcg cagttgcgtg aattgctcac cacattccgc ttgcagctca ccgagcctgg
    17401 attacgtccg gcgctggagg cgagttgcga agagtacagc gccaaatttg gcttcccggt
    17461 gaagctggat tatcaattgc cgcctcgcct ggtgccttcg catcaggcaa tccacttgtt
    17521 gcaaattgcc cgtgaggcat taagtaacgc cctcaaacat tcgcaagcga gtgaagtcgt
    17581 ggtgacggtg gcgcaaaacg ataatcaggt caaactgacc gtccaggata acggctgcgg
    17641 cgtgcctgaa aatgccatcc gcagcaatca ctacggcatg ataataatgc gcgaccgtgc
    17701 gcaaagttta cgaggcgatt gccgcgtccg ccgtcgtgaa tcaggtggca ccgaagtggt
    17761 ggtcaccttt attcccgaaa aaactttcac agacgtccaa ggagataccc atgagtaatc
    17821 aggaaccggc tactatcctg ctgattgacg atcacccgat gctgcgaact ggcgtaaaac
    17881 agcttatcag tatggcacca gatatcaccg tggttggcga agcgagtaat ggcgaacagg
    17941 gtattgaact ggcggaatct cttgatcccg atctgatcct gttagatctc aatatgcccg
    18001 gcatgaacgg tctggaaacg ctggataaac tgcgcgaaaa gtccctctca gggcgcattg
    18061 tggtattcag cgtctctaac catgaagaag atgtggtcac cgcactgaaa cgcggcgcgg
    18121 atggttatct gttaaaagat atggaaccgg aagatctgct gaaagcattg catcaggcag
    18181 ctgctggcga aatggtatta agcgaagcat taacgcctgt tctggccgcc agcttgcgcg
    18241 ctaaccgtgc cactactgag cgcgatgtta accagttaac cccacgcgag cgcgatattc
    18301 tcaagctgat tgcccagggt ttgccgaaca agatgattgc ccgccgcctg gatatcaccg
    18361 aaagcacagt aaaagtgcac gtcaagcaca tgctgaagaa aatgaagctc aagtctcgcg
    18421 tggaagcagc ggtatgggtg catcaggagc gcattttctg attacggttc ccagcgcagt
    18481 tcgtcgtttg acaatgcatc gaacggttcg acaagcgtta gcgtgatctc attggaggag
    18541 acacgctgcc cctggttatc ttccaccacc accgacaatc gccagtgatt gctcgcccct
    18601 tccccgttct gccagtcagg catgatcagc gtccagccct ccgcgctgtt ggcttgtgcg
    18661 cccggcgtca aacttaatat ctgcgtatcg ccctgccaaa tcagttgccg aataccgtaa
    18721 cgactgcgga tttgtaattt cagcggcact gtttcgccag gttttagatc ccacggcggt
    18781 gtcgccagaa acaccgttaa cgtttttcgc tgtcggtact caagagtcgg tagattattt
    18841 cgctgcggat tatcatagcg actaccacgt aacgactgac tttcggcaac ctcgcccgca
    18901 gaaagttgtt ttttgagcgg cacaccaaag cggtaattaa gattcagtcc gaggttattt
    18961 tgattttcgc cactttcacc ctgtttatgc tgggccgtca cagtgactaa tggcacaggg
    19021 gtgtaattta atcccagact caacgcgacg ggattgtgat aacccgtacc agagttaaac
    19081 aaatcaacac gctcaccaaa atactgttct accctgacac tggtattgag gtgttgatag
    19141 aacggcatgc gcatccgggc tgtcaggtca tacccgcgcg ccatccgttg ttcctgcgtg
    19201 gctgtctgtt catgccatgc agcaaacggc tgataaaagt ttgccgatag tcgcaaatat
    19261 tcgccccacg cttcggcacc aaagcccgct cgctgaagat tttcgtccag caagttgtca
    19321 taaaaagtgt tataacccac cagccagttg ccgcgcgccc agcgttgccc aacgcccaca
    19381 ttgctcacca acccatcatc ctgctgagta agaccaagct ggctccaggt gagataacga
    19441 tcattatctt gtaacggcac aaaccagctt ccacgactgc cggtgaaatg tccttcgtta
    19501 tccactttga cgtcaacact ggcatttccc cacggtgata gccaggactc tacgtgctga
    19561 ttaacctgtt gactaagcgc gtcgcggact tttcccaatg cgaaagcttt tgcctgctcg
    19621 ccagtatcca gcccgttatc attcatactg gtttcgccga aatctttcac aatttcagca
    19681 aagtgttttt ccccatcatg gttttcggga gccatgccta aatccggcag accatcattg
    19741 ttattatcaa agggatttgc cgctttttgc tcgaaggtag attgtgcgtt agctgtaccg
    19801 cctgccgcca gcaagagtaa ataaaacggg ataatgcgag gaacgaaacg gctcaaaacg
    19861 tcggtaactt ccggtaataa tgaaaaacca tagccttaaa gaataacgat tactggcaac
    19921 ttttgtcgta ttttctcgcg ctttcaggaa aatccttaaa aaggtgaccg attggtcaga
    19981 cgctgcagaa gcagtaagat agaagatgag tgtctaattc cctgaataca ggagtaaata
    20041 atgcaaaaaa tcgtgatcgt tgccaatggc gcaccttacg ggagcgaatc cttgtttaac
    20101 agcttgcggc tggccattgc gttacgagag caggagagca atctggatct gcgtctgttc
    20161 ctgatgtctg atgcggtcac agccgggttg cgcgggcaaa aaccagggga aggctacaac
    20221 attcagcaaa tgctggagat ccttaccgct cagaatgtac cggtgaaatt gtgcaaaacc
    20281 tgtaccgacg ggcgcgggat tagcacactt cctcttattg atggggtgga aatcggtact
    20341 cttgtggaac tggcgcaatg gacgctgtca gccgataaag tgctcacatt ttaatgtttg
    20401 agggggcgtc tgacgcccca ctatgaggga agtttactca ggcgaatccc ggccgtaaca
    20461 caccatccgg ataattctcc gccagcagtt tttttaccga taccagcaaa tcattcagcc
    20521 catcgtcctg cattcccagt ttgataagct cctgttccag tgaaaacagg tattgtgcgt
    20581 tggttcccag cggaccgctc gccgccgcaa tcaacggcgc gatgacctga gcgcgagtgt
    20641 cagattcata ttctggatgt cgcgggtcca taataaacac aatggcgttt actgtgcgtc
    20701 catcatcaag atcaagctga caccaggttg gcagataaca gccggtaatc atctcgcgct
    20761 tccacaacag ggttagttcc tgctccagcg tctcttctgg tagtcgatag gcgacgcctg
    20821 tggtgcgtcc gccctctttc agtgcaagca tccgtcccgg ctggtgcgca gtcccgcgcc
    20881 cggcggtcag gcgcaggcaa aacgcgcgat gccatccaac cagtgtaccg gtgcataatt
    20941 cggtaaactc cagtgccgga ttccacatca acgatccata accgaagatc cacaccggtc
    21001 cctcattagg tcgacaagcc agcgtcgccg ccagcgaagc cgcccgttgt tctgctgacc
    21061 ataagagtga ttcttcaatg gcaccaaatg ccgttttaca atcggcattc atcaagaaat
    21121 cacgcgttat caccttacac ctccgccact gcatacttcc ctgcgacaac tttttggtgt
    21181 cttccggaca tttttaccgc tcattttgaa accactaacg ggacaatatg caatccacgg
    21241 accacttgca aggcaacacc gcacgaatag ctttaactaa gctaccggtt ttacgatttt
    21301 ttatgccatt tatcatcatc ccctttggca tattcatgct tcacagcagc ccaggccact
    21361 ttatgcgcgg tttcttcgcg actggcgtca tcacgccgat cttctttatc tttatattga
    21421 tcccatgcgc tgttgaacgc ttctttatag atatcctggg catgagacgg tagaacgtgc
    21481 tttacgcttt ccggcagatc gcttttcgtt ttatacggca tcgttgactc cttttacaaa
    21541 gttgaacatt aagtgtggtc aacaatatct actcttgcca aaacataaga attttcatgt
    21601 cagactgttc ttaagaaacg ctaaaacaaa cattgtctaa acgtaatact tatttttatg
    21661 ccccaatgag taaaaaatcg acagcggggt aaaaattcgt aaaatgttac cgatatccag
    21721 acactttctg ttatttttcc tacctatttt ttacattttc gataaattca tctgcaaagg
    21781 gataaccata atgtcaaatg ctcaagaggc ggtaaaaacc cgccacaagg agacttcgct
    21841 tattttcccg gttctggcgc tggtagtgct gttcctgtgg ggaagcagcc agacactacc
    21901 agtggtcatt gccatcaatc ttcttgcgct tattggtatt ttaagtagcg cctttagtgt
    21961 tgtccgtcat gcggacgtat tagcccatcg cctgggagaa ccttacggtt cgcttattct
    22021 tagcctttca gtggttattc ttgaagtcag tttgatttca gctttaatgg caaccggcga
    22081 cgccgcgcca acgctaatgc gtgatacgct ctattcaatc attatgattg ttaccggtgg
    22141 gctggttggc ttttcattat tgttgggcgg tcgtaagttt gccacccaat atatgaatct
    22201 gtttggtatc aagcagtatt taattgcgct gttccccctg gcgataatcg tactggtatt
    22261 tccaatggct ctgcctgcgg cgaatttttc aaccggtcag gcgttactgg tagcattaat
    22321 ttctgcggca atgtatggcg tatttttgct gatccagacc aaaacgcatc aaagtttgtt
    22381 tgtctacgag cacgaagatg acagtgatga cgacgacccg caccacggta aaccgtctgc
    22441 ccatagcagc gtgtggcatg ctatctggtt gattatccat ctgattgccg ttattgcagt
    22501 caccaaaatg aatgccagcc cgctggagac attgctcgac agcatgaatg cccctgtcgc
    22561 ctttactggc ttcctggtgg ctctgttgat tctgtcgccg gaaggtttag gtgcgttaaa
    22621 agcagtgttg aacaaccagg tccagcgcgc aatgaatctg ttctttggtt cagtgttagc
    22681 aaccatttcg cttaccgtac ctgtcgtcac gctaattgcc tttatgacgg gtaacgaatt
    22741 acagtttgca cttggtgcgc cagaaatggt ggtgatggtg gcctctttag tgctgtgcca
    22801 tatctccttc tccaccggac gtactaacgt gctcaatggc gcagcgcatc tggcactgtt
    22861 tgccgcctat ttgatgacga tatttgcctg acagttatat ctcctccgat gacggaaatc
    22921 gggggagata agataaattc ctgcttattg tatttcttca tactacctgg acagctcgtt
    22981 aatcagaatt gcgagcctgt accacaacct gcacgaaacc actgcaacag ctgttgaaaa
    23041 gccggaatgt actctttatt gcaaacattt cagggatgaa gtgaagatga attgagacgt
    23101 agtatccaca ccaagccgga
//
LOCUS       DAJTZL010000054        22467 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.054, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000054 DAJTZL010000000
VERSION     DAJTZL010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22467
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.054"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..418)
                     /gene="ssb1"
                     /locus_tag="OO544_001818"
     CDS             complement(<1..418)
                     /gene="ssb1"
                     /locus_tag="OO544_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HCR6767431.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQP"
     gene            672..3494
                     /gene="uvrA"
                     /locus_tag="OO544_001819"
     CDS             672..3494
                     /gene="uvrA"
                     /locus_tag="OO544_001819"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HCR6767432.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(3529..3885)
                     /locus_tag="OO544_001820"
     CDS             complement(3529..3885)
                     /locus_tag="OO544_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCR6767433.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(3889..4305)
                     /locus_tag="OO544_001821"
     CDS             complement(3889..4305)
                     /locus_tag="OO544_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HCR6767434.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PLHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(4416..5129)
                     /gene="aphA"
                     /locus_tag="OO544_001822"
     CDS             complement(4416..5129)
                     /gene="aphA"
                     /locus_tag="OO544_001822"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HCR6767435.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            5531..5753
                     /locus_tag="OO544_001823"
                     /pseudo
     CDS             5531..5753
                     /locus_tag="OO544_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165333.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6257..7450)
                     /gene="tyrB"
                     /locus_tag="OO544_001824"
     CDS             complement(6257..7450)
                     /gene="tyrB"
                     /locus_tag="OO544_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HCR6767436.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNSVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     SAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(7703..8782)
                     /gene="alr"
                     /locus_tag="OO544_001825"
     CDS             complement(7703..8782)
                     /gene="alr"
                     /locus_tag="OO544_001825"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HCR6767437.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSIGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            complement(8835..10250)
                     /gene="dnaB"
                     /locus_tag="OO544_001826"
     CDS             complement(8835..10250)
                     /gene="dnaB"
                     /locus_tag="OO544_001826"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCR6767438.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            10333..11316
                     /locus_tag="OO544_001827"
     CDS             10333..11316
                     /locus_tag="OO544_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HCR6767439.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREEDLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            complement(11482..11724)
                     /gene="pspG"
                     /locus_tag="OO544_001828"
     CDS             complement(11482..11724)
                     /gene="pspG"
                     /locus_tag="OO544_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HCR6767440.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKVPKYQRYDRWRY"
     gene            complement(11858..12895)
                     /gene="dusA"
                     /locus_tag="OO544_001829"
     CDS             complement(11858..12895)
                     /gene="dusA"
                     /locus_tag="OO544_001829"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HCR6767441.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            complement(13258..14243)
                     /locus_tag="OO544_001830"
                     /pseudo
     CDS             complement(13258..14243)
                     /locus_tag="OO544_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560258.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 domain-containing protein"
     gene            14561..15076
                     /gene="zur"
                     /locus_tag="OO544_001831"
     CDS             14561..15076
                     /gene="zur"
                     /locus_tag="OO544_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HCR6767442.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            complement(15118..15327)
                     /locus_tag="OO544_001832"
     CDS             complement(15118..15327)
                     /locus_tag="OO544_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HCR6767443.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(15443..16768)
                     /gene="dinF"
                     /locus_tag="OO544_001833"
     CDS             complement(15443..16768)
                     /gene="dinF"
                     /locus_tag="OO544_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HCR6767444.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMSKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            complement(16841..17449)
                     /gene="lexA"
                     /locus_tag="OO544_001834"
     CDS             complement(16841..17449)
                     /gene="lexA"
                     /locus_tag="OO544_001834"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HCR6767445.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            complement(17559..17927)
                     /gene="dgkA"
                     /locus_tag="OO544_001835"
     CDS             complement(17559..17927)
                     /gene="dgkA"
                     /locus_tag="OO544_001835"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HCR6767446.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            18098..20521
                     /gene="plsB"
                     /locus_tag="OO544_001836"
     CDS             18098..20521
                     /gene="plsB"
                     /locus_tag="OO544_001836"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HCR6767447.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRPLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            complement(20676..21548)
                     /gene="ubiA"
                     /locus_tag="OO544_001837"
     CDS             complement(20676..21548)
                     /gene="ubiA"
                     /locus_tag="OO544_001837"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HCR6767448.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSILVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(21560..22057)
                     /gene="ubiC"
                     /locus_tag="OO544_001838"
     CDS             complement(21560..22057)
                     /gene="ubiC"
                     /locus_tag="OO544_001838"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HCR6767449.1"
                     /translation="MSHPALTQLRALRYFKEIPALDSQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            complement(22280..>22467)
                     /locus_tag="OO544_001839"
     CDS             complement(22280..>22467)
                     /locus_tag="OO544_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6767450.1"
                     /translation="MLNPTKDTNWNSTCIYKSRHKMLPINLTQETLFSSKSHGKYALF
                     PIFTASWRATRIKNIGI"
BASE COUNT         5771 a         5841 c         5430 g         5425 t
ORIGIN      
        1 gaggctgccc ccaaccgccc tgcggctgac caccaccgat attaccacct gccggagcgc
       61 caccaccctg acgaccaccc agcatctgca tggtgccgcc aacgttcacc acgacttctg
      121 tggtgtagcg atcctgaccg gattgatcgg tccatttacg ggtacgcagc tgaccttcga
      181 tataaacctg agaaccttta cgcagatatt cgctggccac ttctgccagt ttgccgaaca
      241 gcacaacgcg gtgccattca gtctgttctt tcatctcgcc ggtcgcttta tcacgccagg
      301 attcggaagt agccagcgta atgttggcaa ctgcgccacc atttggcatg tagcgtactt
      361 ccgggtcctg acccagatta ccaacgagaa taaccttgtt tacgcctctg ctggccatgt
      421 tcgtgtctcc tgaaaaaaat cgttctgaat aagtgtaaac gcgcgattgt accattacca
      481 atagcgcttt tactatgttg tgacctcggt tccgggaaac aaacctggcc agacattgtt
      541 acacaacact ccggataatg cattccaata ctgtatattc attcaggtca atttgtgtca
      601 taattaaccg tttgtgatcg ccggtagcac catgccaccg ggcaaaaaag cgtttaatcc
      661 gggaaaggtg aatggataag atcgaagttc ggggcgcccg cacccataat ctcaaaaaca
      721 tcaacctcgt tatcccccgc gacaagctca ttgtcgtgac cgggctttcg ggttctggca
      781 aatcctcgct cgctttcgac accttatatg ccgaagggca gcgccgttac gttgaatccc
      841 tttccgccta cgcgcggcag tttctgtcac tgatggaaaa gccggacgtc gatcatattg
      901 aggggctttc tcctgccatc tcaattgagc agaaatcgac gtctcataac ccgcgttcta
      961 cggtggggac aatcaccgaa atccacgact atttgcgttt gttgttcgcc cgcgtcggcg
     1021 aaccgcgctg tccggaccac gacgtcccgc tggcggcgca aaccgtcagc cagatggtgg
     1081 ataacgtgct gtcgcagccg gaaggcaagc gtctgatgct gctcgcgcca atcattaaag
     1141 agcgcaaagg cgaacacacc aaaacgctgg agaacctggc aagccagggt tacatccgtg
     1201 ctcgtattga tggcgaagtc tgcgatcttt ccgatccgcc gaaactggaa ctgcaaaaga
     1261 aacacactat tgaagtggtg gttgatcgct tcaaggtgcg tgacgatctt acccaacgtc
     1321 ttgcggagtc gtttgaaacc gcgctggagc tttccggtgg tacagcggta gtggcggata
     1381 tggacgaccc gaaagcggaa gagctgctgt tctccgccaa cttcgcctgc ccaatttgcg
     1441 gctacagtat gcgtgaactg gagccgcgac tgttttcgtt taacaacccg gcaggtgcct
     1501 gcccgacctg tgacggcctt ggcgtacagc aatatttcga tcctgaccgc gtgatccaaa
     1561 accccgagct gtcactggct ggcggtgcga tccgtggctg ggatcgccgc aacttctatt
     1621 atttccagat gctgaaatcg ctggcagatc actataagtt cgacgtcgaa gcgccgtggg
     1681 gcagcctgag cgcgaacgtg cataaagtgg tgttgtacgg ttctggcaaa gaaaacattg
     1741 aattcaaata catgaacgat cgtggcgata cctccattcg tcgtcatccg ttcgaaggcg
     1801 tgctgcacaa tatggagcgc cgttataaag agacggaatc cagtgcggta cgtgaagaat
     1861 tagccaagtt tatcagcaat cgcccatgcg ccagctgcga aggaacgcgt ctgcgtcggg
     1921 aagcgcgcca cgtgtatgtc gagaatacgc cgctgcctgc tatctccgac atgagcatcg
     1981 gtcatgcgat ggaattcttc aacaatctca aactcgcagg tcagcgggcg aagattgcag
     2041 aaaaaatcct taaagagatc ggcgatcgtc tgaaattcct cgttaacgtc ggcctgaatt
     2101 acctgacact ttcccgctcg gcagaaacac tttccggcgg tgaagcccag cgtatccgtc
     2161 tggcgagcca gattggtgcg ggcctggttg gcgttatgta tgtactggac gagccgtcta
     2221 tcggcctgca ccagcgcgat aacgagcgcc tgttgggtac gcttatccat ctgcgcgatc
     2281 tcggtaatac cgtgattgtg gtggagcatg acgaagacgc aattcgcgcc gctgaccatg
     2341 tgatcgatat cggtcctggt gcgggtgttc acggcggtga agtggtcgca gaaggtccgc
     2401 tggaagcgat tatggcggtg cctgaatcgt tgaccgggca gtacatgagc ggtaaacgca
     2461 agattgaagt gccgaagaaa cgcgttccgg cgaatccaga aaaagtgctg aagctgacag
     2521 gtgcacgcgg caacaacctg aaggacgtga cgctcacgct gccagtcggt ctgtttacct
     2581 gcatcacagg ggtttcaggt tccggtaaat cgacgctgat taacgacaca ctgttcccga
     2641 ttgcccaacg ccagttgaat ggtgcgacca tcgccgaacc ggcaccgtat cgcgatattc
     2701 aggggctgga gcatttcgat aaagtgatcg atatcgacca aagcccaatt ggtcgtactc
     2761 cacgttctaa cccggcgacc tataccggcg tgtttacacc tgtgcgcgaa ctgtttgcgg
     2821 gcgtaccgga atcccgtgcg cgtggttata cgccaggacg tttcagcttt aacgtccgtg
     2881 gcgggcgctg cgaagcctgt cagggcgacg gtgtgatcaa agtggagatg cacttcctgc
     2941 cggatatcta cgtgccgtgc gatcagtgca aaggtaaacg ctataaccgt gaaacgctgg
     3001 aaattaagta caaaggcaaa accatccacg aagtgctgga tatgaccatc gaagaggcgc
     3061 gtgagttctt tgatgcggtg ccagctctgg cgcgtaagct gcaaacgttg atggacgttg
     3121 gtctgacgta cattcgcctg gggcagtccg caaccacact ttctggtggt gaagcccagc
     3181 gcgtgaagct ggcgcgtgag ctgtcaaaac gcggcaccgg gcagacgctg tatattctcg
     3241 acgagccgac caccggtctg cacttcgccg atattcagca actcctcgac gtgctgcata
     3301 aactgcgcga tcagggcaat accattgtgg tgattgagca caatctcgac gtgatcaaaa
     3361 ccgctgactg gattgtcgac ctgggaccag aaggcggcag tggcggcggc gaaatcctcg
     3421 tctccggtac gccagaaacc gtcgcggagt gcgaagcttc gcatacggca cgcttcctca
     3481 agccgatgct gtaatcgtta aggccgcttt ctgagcggcc ttttcctttc agagttgtac
     3541 cagcaattta cgcttttctt ccggtagtaa attcactgcc tgctgataag acgcatccac
     3601 cagataatag atttgcgaat ccggcagcga accgtcgaga tacacggtgc tccagtgcgc
     3661 tttattcaga tggcggctag gacgcacatc gctgtgctgc tgacgtagca gctccgccag
     3721 ctccgggctg gttttcagcg aaacagctgg gcgattttct acttctttca ccatcgcaaa
     3781 cagtacatct tccactttga tctgcgtcgc tttccagtcg ttatgcacgc tctgttctgc
     3841 gcctggtttt gccatgcaat attgtagcaa ctccgaaatg gtcatttttt actccccttg
     3901 tagtgtcgcg atgatgcgac gcgatccgcc gtggatgcga tgttccccca gccagatgcc
     3961 ttgccaggtg ccggtctgaa tacgcccttt atgtaacggc aatacaagcg atgttcccag
     4021 cattgaggat ttgatatgag aaggcatatc gtctgctccc tcatagtcat gctcataatt
     4081 tccgttgtcg ggaacggtgc ggaggaaaaa acgctccatg tcgtggcgta cggtgggatc
     4141 gcagttctca ttaagtgtca gagaggcgga ggtatgttgc agcaacagat gcagtaagcc
     4201 gatgttaacg cgcggcatat cagccagctg attcagaatt tcatccgtta ccagatgaaa
     4261 cccacgagat ttggcgctaa gcgtgagcgt cttttgatac cacatgtgct gctccttgat
     4321 aaaactctct taatcagttt gcagcaagac agcgaaagga taaaggtgtg attaaaaaaa
     4381 cagcattcgg gagagcatca cgctctcccg ctctgtcagt attctgaatt gacgatcacc
     4441 tcttcaccaa acgcacccgc ttgtggcaag ggtttgtagg tagagttgga ggcgcgcaga
     4501 atgcggatac cacgagcgcc gacatcgcgt gcggcggtaa tatcgttatc ggaatcgcca
     4561 taaaaaattc ggatattttt atcctgcagc cattgcgatt ttgtattttg ccctggttta
     4621 tcgcccgcaa agattaccgg attcatgttg gtggcaggaa tatgaaaatt atccgccagc
     4681 gtttttgaaa ccgtttctgt tttcgtcggg ctacgaccag tcacaaagaa gatcgcgtca
     4741 ccgcggcgta catgcatatc aatcagctgg cgagcgacct cttttggaat gctgaattca
     4801 tcccagccat tattcatttt ttcccagaac acaggatttt tcagataatc ttcgctttct
     4861 ggcgagaagg tttttttgcc gcgccagaac cccggactgg aaaaaagtac cgtgtcatcg
     4921 atatcaaacc ccaccgccat tggcggacgc cctgcgaggc tattttcaat ttgtgcgacc
     4981 gaaacccaat gaattggtgc ctgttcagca agcctggcaa cgttagtccc agggttaagc
     5041 ggtgaaggag atgaggccag ggcaacagcg gaactgttta gagcgaacaa taagcaaacg
     5101 gcactgattg cctgtgtgat cttgcgcata tttttcccta aatagtcagt atgttgaaac
     5161 tttttattgt gagatttgtt gcaaaaacca tctgaccata acgccagctg ccggggaaag
     5221 gaaggattat ctgcggtttt tgtgatgaaa agcaggatga ttatatttaa cccagtaaat
     5281 acgttcctgt ctttttttgt ataaaaaatg tttattactc gatgtaagga tacattaatt
     5341 ttacgtttat tatgatgaat cctctgcaaa tgagcgaaat ttgaaagaac atttatctgc
     5401 tgtacgattt aatgaactta atgatacagc attacaaaaa caatcgaagt ttataaagat
     5461 gattcctgat tgaccagaac cctatcctac aatggtgact aattttatta ctcccgatgg
     5521 agatgatgat atgaatatat catatgtgaa tagcaacaaa acgacatctt tacctgttga
     5581 gctagatgca ctaaataata aagacatctc ttatgccaaa gatttttctt atgctaaaga
     5641 tttcttttta tacatagaaa cacagttaaa aattgcaaaa gatttttgta gacctggaga
     5701 agaagtatca agttctattg caagtaaagt ttttcacgca tttatcgatt tagttaacaa
     5761 aatcaggggt aagaaagact ttatgtatat ttgcacgctt tgctgttttg ccgaggaggt
     5821 taaaggtgat tattctcatt acaggacctt tttatttgat attggtaatc aatataaggt
     5881 taaacttaca caaagcggaa aaaaagagtt ctctttaaca ttagaattta acgatactat
     5941 aattgaatct cagaaagtca caggcaataa ggcaaagtat attcttgagg atatagaaat
     6001 attctatcgt aacaaacccg atacttatta ttaaatttaa taatggttag atgtgcagaa
     6061 attattactc cacacgaaaa aatagggaaa ggaattccag ataattggga taaacttaac
     6121 aataaaagaa ctgacaacgt tttattatct ctcacgtaga acaacggcat caaaaaaatc
     6181 atctacaaag aaatagtgaa agcgacgaca gtttaatttc actgcaggct gggtaactcc
     6241 agcctgcttt cctgcattac atcaccgcag caaacgcctt tgccacacgt tgtacatttg
     6301 ccgtatttaa cccggcgaca cacatgcgac cgctggcgat gagatagaca ccaaattctt
     6361 cacgtagtcg gtcaacctga gcggcactta aaccggtata actgaacatg ccgcgctgat
     6421 taagcagata atcgaaattg cgttctggca tctctgtgct taataccttc accaattcct
     6481 gacgcattgc cagaatgcga gtacgcatct cttctacttc cgccagccag ctggctttca
     6541 atgcctcgtc attcagcact gcagccacca cctgcgcacc aaaattcggc gggctggagt
     6601 agttgcggcg aactgttgct ttcaattgcc ccagtacgcg gccagcggct tcggcatctt
     6661 cacacagaac agaaagtccg ccgacgcgct cgccgtaaag ggagaaaatt ttcgagaacg
     6721 aattgctcac cagagcgggt aatccagcgc tggcaatggc gcgaatagcg taggcatcct
     6781 cttccatacc ggcactaaat ccttgatagg caatatcgag gaatgggata agctcgcggg
     6841 ctttgagaat ttcaatcacc gcatcccact gatcattagt gagatcggca cccgttgggt
     6901 tgtggcaaca tggatgcagc aacacaatac tgcgggcagg taatgttttc agcgtcgcca
     6961 acaggtcatt aaagcgcacg ctgttagtcg cttcgtcata ccaggggtaa gtactcactt
     7021 cgaatccagc cccggcgaat attgctacgt ggttttccca ggtaggatcg ctgacccaga
     7081 cgcctgattc cgggaagtag cgtttcagga aatccgcgcc cactttcaat gcccctgagc
     7141 cgccaagggt ttgaatggtt gctacgcgct gttgttgcag taccggatgg tccgcaccaa
     7201 acagcagcgg cgcaatggca tggcgatagc tgttaagccc ttccatcggt aaataaagcg
     7261 aagcgccatg gggctgcgca ttcaggcgcg cttccgcctc cgccacggct ttcagttgtg
     7321 gaataattcc gtcttcgttg tagtacagac cgatacttaa attcactttg tcgctgcgag
     7381 ggtcttcttt aaaacgctcc ataagcgtaa gaatcggatc gccagcgtag gcgtcaactt
     7441 tttgaaacac gcgatggttc tccaggttta cgggcaggtg gttaaaacac aataaaccgg
     7501 aagaaggcga agatcgagtg gatgttcagg agcgaacggc aattagcaac agagtgagac
     7561 tcatgacaaa cgaacatccg ccagaggcac gacctttata agagcatgaa agaacatcaa
     7621 cttattgaat ttttaggatt ttattggccg gataaggcat tcacgccgca tccggcacag
     7681 acaatcaaat attacagaac gattaatcca cgtatttcat cgcgaccctt gaagtcaggc
     7741 gcgtaataag ttcgtaagcg ctcacttttg tcatttcagc gatacgttct acgggcaaac
     7801 cttcgcccca taaaatgacc ggatccccgg ctttgtcctg cgcctgtgga cctaagtcta
     7861 cgcagatcat atccatcgcc acgcgtccga caatcggtac ttcgcgaccg ttcaccagca
     7921 ctggcgtacc ggatggagcg gcgcgcggat aaccatcgcc atagcccatc gcgactacgc
     7981 caagacgggt atcacgttcg cttacccagg ttccaccata accaacaggc tctccggctt
     8041 tatgctcacg cacggcaatc aggctggagg ttagtgacat cactggctga cagccaaaat
     8101 cggcaccgat ggagcgatct tccagcggcg agacgccata aagaatgatg cccgggcgca
     8161 cccagtcaaa atgcgattgt ggccacagca gaatgccacc cgacgcggca atggaacgtt
     8221 gaccaggttt gccttcgcaa aaggtattaa agatagcgag ttgtttctcg gttgcgccac
     8281 attttggttc atccgcgcgc gcaaaatggc tgacgatatt caccggctga cgaacgtttt
     8341 tgcactgtgt caggcgatga taaaacgcct cagcctgttc cggccttacg cccagacggt
     8401 gcataccggt atcgagtttc atccagacgg taaccggctc gtccaggcta gcctcttcca
     8461 gcgcagccag ctgttcttcg ttatgcacgg cggtatgaaa atgttgcgca gaaatcgtcg
     8521 gcagatctct ggcatcaaaa aagccttcga gtaacagtac aggtttggtg attccccccg
     8581 cacgcagtcg cagagcttct tcgagacggg ctacgccaaa ggcgtcagca tcggggagcg
     8641 ttcgcgcggt ctcaagaaga ccgtgaccat aagcgttcgc tttcaccacc gcaaccattt
     8701 tactggcagg ggccagttca cgaagacgtt gcaggttgtg tcgcagagcg cggcggttaa
     8761 tcacaacagt tgccgcttgc atttgtgttc cttgataagt gtttgcttta attacctaat
     8821 tcataaaata attattattc gtcgtcgtac tgcggccccg catagttgtc gaagcgcgac
     8881 cattgaccgt taaaggtcag gcgtaccgtc ccgattgggc cgttacgttg tttaccgata
     8941 ataatttccg cgatgccttt taaatcactg ttttcgtgat acacctcatc acgatagata
     9001 aacataatca agtccgcatc ctgctcgata gagccagatt cacgcaggtc ggagttgacc
     9061 gggcgtttgt cggcacgttg ttccagagaa cggttcaact gggacagcgc caccaccggc
     9121 acgttcagtt ctttcgccag tgctttcagc gagcgagaga tttctgcaat ttccagcgta
     9181 cggttatcgg aaagcgccgg tacgcgcatc agttgcaggt agtcgatcat gataagcccg
     9241 atgccgccgt gttcacgggc aatacggcgt gcgcgggaac gcacttccgt tggcgtcagg
     9301 ccggaggagt catcgatata gatattgcgt ttttcgagca aaatacccat ggtgccggaa
     9361 atgcgcgccc agtcttcatc atcgagctgc ccggtacgga ttttagtctg gtcaacgcgc
     9421 gacagcgacg ccagagaacg catcataatc tgttctgaag gcatctccag cgagaagata
     9481 agtactggtt tatcctgcaa catcgccgcg ttttcgacga ggttcatcgc aaatgttgtt
     9541 ttacccatcg acggacgcgc ggcgacgatg atcaaatccg acggctgcaa gccagcggtt
     9601 tttttgttga gatcgtcata accggtgttt actccggtaa cgccatcgtg tggctgctga
     9661 aacaactgct caatacgcgc cacggttgcg tcgagcacat cggcgatgtt cttcggccct
     9721 tcgtctttgt ttgcacgact ttcggcaatt ttaaagacgc gggattcagc aaggtccagc
     9781 agatcttcgc tggtacgccc ctgcggatca aaaccggctt cagcaatctc attcgcaacc
     9841 gagatcatct cacggacaac ggcacgttca cgcacgatgt cagcataagc actgatgttc
     9901 gccgcacttg gcgtattttt tgacagctct gccagataag caaaaccacc gacgctatcg
     9961 agttgcccct ggcgttccag cgattccgca agggtaatca gatcgatagg actaccgctt
    10021 tcctgcaaac gcgccatttc agtaaagata tggcggtgtg ggcgggtgta aaaatcgtct
    10081 gccaccacac gctcggctac atcgtcccag cgttcgttat ccagcattaa accgcccaac
    10141 accgactgct ccgcttcgat cgagtgcgga ggcactttca gcccggcaac ttgtggatcg
    10201 cgttcgcggg gttcagcctg ctgtttgttg aagggtttat ttcctgccat agtgaatgga
    10261 gttaccgaga taaagaatgg gtcgaaactt taccatatga agcagaccct gacgatacgt
    10321 tctggaggac acatggcaac acgaattgaa tttcacaagc acggtggccc ggaagtactt
    10381 caagccgtag agttcactcc tgccgatccg gcagagaatg aaatccaggt cgaaaataaa
    10441 gccatcggca tcaattttat cgacacgtat atccgcagcg gcctttaccc gccgccatca
    10501 ctacccagcg gattaggtac cgaagcggca ggcatcgtga gtaaagtcgg cagtggtgta
    10561 aagcatatta aggcaggcga tcgtgtagtc tatgcgcagt cggcgttagg cgcttacagc
    10621 tctgtgcata acattaatgc ggataaagcg gcgattctgc ctgcggcaat ttcttttgag
    10681 caagctgcgg catccttcct gaaaggctta acggtttatt atctgctgcg caaaacctat
    10741 gaaattaaac ccgatgaaca gttcctgttc cacgcagcgg ctggcggcgt tggcttaatt
    10801 gcctgccagt gggcaaaagc cctgggcgcg aaacttatcg gcaccgtagg aaccgcgcaa
    10861 aaagcgcaga gcgcgctaaa agcgggcgcg tggcaggtta ttaactatcg tgaagaggat
    10921 ctggtcgagc ggttaaaaga gatcaccggc ggtaagaaag tgcgcgtggt gtacgattcc
    10981 gtgggcagag acacctggga acggtcgctg gattgcctgc aacgccgcgg cttaatggtc
    11041 agttttggca actcatcagg tgcggttacc ggtgtgaact taggcattct caatcaaaaa
    11101 ggctcgttgt atgtgacacg cccttccctg caaggctata tcaccacgcg ggaggaatta
    11161 accgaggcca gtaatgaact gttctctttg attgccagcg gtgtgattaa ggtcgatgtc
    11221 gccgagcagc agaaatatcc gctgaaggat gcgcagcgtg cgcatgagat tctggaaagc
    11281 cgggcgacgc aaggttccag cctgttgatt ccataaaaga aatagggctt ccacctggga
    11341 agccctttct ttttatagtt cggctgtatg tagggtacag cacgatgaat ctgttagagg
    11401 cgcaatagtg acagatttga ttatcaattc ctattttgtt ctaaggataa aaccttaggt
    11461 tgtgatcatc cgcacaatcc cttagtaacg ccagcggtca taacgctgat atttcggcac
    11521 ttttggtgct ttaatcgcct taataaccca caccaccgca atcgccagta gtaaccacgg
    11581 cagcaactta atcatcaatg ccagcatacc gccgaggaac ataatggccg tcgccacaac
    11641 cagcgcggcg ataatgccca gcaacgaaac gccggtgacc atcagcatga caaaaaagcc
    11701 aatcacaaaa agtagttcca gcatgatgct ctcccaaata tgaaatctct tgctggcatt
    11761 acaagaatca tgccaaaaat aatctattga tttaacagca aaacgccccg cgacggtgcg
    11821 cagggcgtgg tgaatttgac tactttttgg tgaaaagtta acgcttatcc gccaccagtt
    11881 tgagcgcgtg ttccagcaca ttaatgtctg cacccgcttt atgggcattt tcacttaaat
    11941 aacgccgcca ctgccgcgcg ccaggaatac cctggaacaa gcccaacata tgccgggtaa
    12001 tatggccgag atacgtcccc tggctgagtt cacgctcaat gtacggatac atggcgcgca
    12061 ctaccgccac cggatcggca tcggtatccg aggaaccaaa gatctcccgg tctaccgccg
    12121 ccagaatacc cggattctga tacgcctcgc gcccgaccat cacgccatcc atatgttgca
    12181 ggtgtgcttt ggcctcttcc agcgacttga taccaccgtt aatcgacatt gtcagatgcg
    12241 gaaagtcacg cttcagttga tacacacgcg gataatcgag cggcgggatt tcacggtttt
    12301 ctttcgggct taaccccgaa agccaggctt tacgtgcgtg gatgataaac atctcacact
    12361 cgcctttgcc ggaaacggtg ttgatgaaat cgcagagaaa ttcatagctg tcctggtcat
    12421 cgatgccaat acgcgttttc accgtcactg gaatcgacac cacatcgcgc atcgctttca
    12481 cgcagtcggc aaccagctgc gcattaccca tcagacacgc accaaacatg ccgttctgca
    12541 cccggtcaga cgggcagccg acattcaggt tgatctcatc atatccacgc gcttctgcca
    12601 gcttcgcaca ctgtgccagc gccgccggat cgctaccgcc gagttgcaac gctaccggat
    12661 gttcttcttc actgtacgcc aggtaatcac ctttaccgtg aataatcgcc cctgtggtca
    12721 ccatttcggt atacagcaac gtattgcggg aaagcagacg caagaaatag cggcaatgtc
    12781 tgtccgtcca gtcgagcatt ggtgcaacgc taaaccgacc actccagtga acgtcagttt
    12841 tttcaggcat tgcgctggtt tggttgattt tttgcatttc agaattaccg tgcattttca
    12901 aatgtagaga ttattttatc gatatatcat ggggttatgt tattcagcat cactattcag
    12961 gaggctcaat agcggggtac tataccataa caacaggaag cgcctgtctc attgcaaaag
    13021 aaaattgaga tcctctcaag gcatgaagct ctcacgaagt gatggaaata atcttattag
    13081 ccgttaactt tgttaaggcc aatgataaac aatccaggtt cgacgataaa taaaaaatca
    13141 cacattaaac tctggtgata tatctccctg ctaatagcat tgatagagaa aaaagaaccc
    13201 aataaatatt gggtcctttt atataatgcc ttccatacta tcgaagaact tcacatatta
    13261 tttctccgat ttaaccccga acaaatcata aattaattta gaagtgtctg taagtatttt
    13321 aatctcttcc tttgaggttg ggtcaaacga cttcgcgaag ctaattaatt gtggtgcagc
    13381 atctcgcatt ttgcttaaaa tatcaggatc gagcgttcct tcattcacca gatgtgacat
    13441 cttatccaga tagccatcaa aattcatttc tcccccatca gccagacgct tcatctctcc
    13501 caggtacttc ttcatatcac gttgagataa tttttcaaac tgagctttca ggtagctctc
    13561 attattttca ttaatataca ctgtttctga taaatctgcg taggcactag aatatgaaag
    13621 tgaaattaac agtgaagcaa ataattttaa gctgttgtca tcatgttcct ggcaggcatt
    13681 aacaaacgtt aaaaaaccag aacctattgc ttgaaaatgt atattcgcta aaggttcatt
    13741 atccttagat tcctcataaa atagctgaac cgtggaggga aggccattct tagtttctac
    13801 atcaaaagta cattgttcaa tgggagtcgc atctttatta tccccaggat tacatatact
    13861 attaatcgcg aaatgaaaaa tagcccggtc aacaactgat gcaataaagt cacgatcgtt
    13921 taaatcctca tcagtgcaat cgtcaatata cttattgacc cacattttga gatcatttat
    13981 ttgctcaatt aaaaacacat ttttttacgt gcatgccata aattatgcgt gattcgttga
    14041 ataaacaaat ctatttttgc aataattata tcccgatgac gcagcgctgc ataatcaata
    14101 gatttaccat atatattatt taatttacct ttgattgaac ctctattatc taatttatct
    14161 ttgattgcat cttcattatc gaattgattt gaattgagtg ttttattaaa gtatccagtt
    14221 gcaccgggta ttatcttcaa catgattcat ctaccttttt tgtcgtaccg tgtcctcctt
    14281 ataattcttt tgccatctgg tcactatcaa aaatcgctca taatttctga actacaggta
    14341 tgcgtttaaa cccagttaaa taaatagcga gttaacttga agcaatttat ttaaaaatga
    14401 attattaatt attatcgtgt tgataatggt acgacaaggc aacataacac cttacctaca
    14461 aaacagaaaa tcaacattac ggcaacaata agggttctcg gtgtttttcc tggaacatgg
    14521 taaagtaagg acattcttaa cccccacttt gaggtgcccg atggaaaaga ccacaacgca
    14581 ggagttatta gcgcaggctg aaaaaatctg cgcgcagcgt aatgtgcgcc tgaccccaca
    14641 gcgcctggaa gtgttgcgcc tgatgagtct gcaggatggc gctatcagcg cttatgatct
    14701 gcttgattta ctgcgcgaag ctgaaccgca agccaagccg ccaacggttt atcgcgcgct
    14761 ggattttctg cttgagcaag gttttgtgca taaggtggaa tccaccaaca gttatgtgct
    14821 ctgtcatctg ttcgaccagc ccacgcatac gtcagccatg tttatatgcg atcgctgcgg
    14881 cgcggtgaaa gaagagtgtg cagaaggcgt ggaagacatt atgcatacgc tggcggcaaa
    14941 aatggggttt gccctgcggc ataatgtgat tgaagcacat gggctctgtg cggcatgcgt
    15001 ggaagtggaa gcgtgtcgtc atcctgaaca gtgccagcat gatcactctg tgcaggtgaa
    15061 aaagaaaccg cgttaagagg gcgtacatcc ttgtacacgt cgggcaggag ggattaatta
    15121 ccagcgatat tcattgcggg tttcccaatc cacgacctct ttttctgcct gatctttctg
    15181 ataaccataa cgttcctgga ttttaccgac cagttgatca cgtttacctt caatgatcgt
    15241 catatcatca tcggtcagtt tgccccattg ctctttcact ttacctttaa actgtttcca
    15301 gttaccgccg gcttcatctt tattcataat caagacctca tcgttaggtt gtgaatgaga
    15361 gtacgttcac ttttcttctg aacgtgagat taagtgtagt cagcaatttg gcttaggata
    15421 atttattcag aatttttaac cgtcacgttg cggcaaacca ggtaccgtta cgccagtgac
    15481 gccgccagat agccgccaga gaaagcccgc gcaacgccag aaagacggtt agtgccagcc
    15541 acaaaccatg attacccagc cacggcagcg taaggagcgt cagcgcaaaa cctgcggcgg
    15601 ccaccgccat actgttacgc atttcggcgg cacgcgttgc gcctataaac atgccgtcca
    15661 gcagataaca ccagacgcca accaacggca aaatcacctg ccagataaga tagcggtcag
    15721 ccagctgctg aatctgggtt aacgacgtca gcaacgcaat gatgtgttcc cctgccagca
    15781 aataaaccac cgaaaatagt aacgctacga tccccgactg gcggcacgct gcccgccaga
    15841 catccagcaa ctggctaccg tcgcgcgcac cgtatgcctg accggaatgt gcttcaaccg
    15901 cgtaggcaaa accatccagc gcataggcgg taaaggtgag tagcgtcatc agaaccgcgt
    15961 ttacagcgat aatgtcactc cccagtcgcg cgccaagtac ggtgatcgcg ccgaaacaga
    16021 gttgcaacaa cagcgaacgc agcatgatat cgcggttaag cgccagcaag cgacggaagt
    16081 ttcctcgcca ggcagttttc gacatttcgc cggaaattcc gcgtagtttg aggattttac
    16141 gcaccattag cagaccaatc agcaatgttg catattccgc aataaccgtc gccagagccg
    16201 cgccctgcac gttcatatgc agccccatca ctagccagac atccagcaca atgttgagga
    16261 tattgccgac cactaacaaa attactggcg cacgggcata ttgcacgccg agtaaccaac
    16321 cgagtaatac cagattcgcc agcgacgccg gtgcgcttaa ccagcggatt tcaagaaagc
    16381 gccgcgcctg ttccaggact gcttcactac cgccaacaat atgcagagcc agatcgataa
    16441 tcggtgtacg cagcagcgca attaacgccc cagcccccaa cgccaacagc aacggttgca
    16501 ccagcgcacg ggctaatgcc tgaggatttt tggcaccata agcctgcgca gtcagcccgg
    16561 tggtgctcat gcgtaaaaac agcaacagca taaagagaaa gctggtcgcc gttgcaccaa
    16621 ccgccacgcc gcccaaataa accgggctat caagatgacc aattaccgcc gtatcgacca
    16681 gtcccagcaa cggaacggtg atattggaga aaatcatggg taaggcgaga tgccagagtg
    16741 ctttatcaga tgaagtgagg aatgccatgc agacaagcct gatgaagaga gatgaaaaac
    16801 aaaccgcgac gcctggcggc atcgcggtct cagagatatg ttacagccag tcgccgttgc
    16861 gaataacccc aaccgccagc ccttcaatgg tgaagctctg ctgacgaagg tcaacgacaa
    16921 ttggtttaaa ctcgctattt tctggcaaca gttcgacttt attaccctgt tttttcaggc
    16981 gcttaacggt aacttcgtca tcaatacgtg cgacaacgac ctgaccgtta cgtacatcct
    17041 gagttttatg cactgccagc aagtcaccat ccataatgcc gatatctttc atcgacatcc
    17101 cgctgacgcg cagcaggaaa tcagcattcg gcttgaacaa ggaaggatcg acctgataat
    17161 gaccttcaat atgctgctgc gccagaagcg gttcaccggc agccacacga cctaccagcg
    17221 gcaacccttc ttcctcttcc tgcaacagac gaatcccgcg tgatgcgccg gaaacaattt
    17281 caataacgcc tttgcgtgcc agcgccttca gatgttcttc agccgcgttt ggggaacgga
    17341 accccaaacg ctgcgcgatt tccgcacgcg tcggcggcat acctgtctgg ctgatgtgat
    17401 cacggatgag atcaaacacc tcttgttgcc tggccgttaa cgctttcatt ccgccccctg
    17461 ggtgtatata cagttatgct gtgagtatat acagcaaaag gcgattttgg aaccataaac
    17521 tgcacaataa accagagatt tatcgaaatc tggaagggtt atccaaaatg cgaccataac
    17581 aggatgcacc aggttatcac ggcgacgata atcgcaatca gcaccgcggc ggatcccata
    17641 tctttagcgc gtccggaaag ctcatggtat tcagagccaa ttcggtcaac cactgcttcg
    17701 atggcgctat tgagaatttc cacaatcatc accagcatca cggagctgat aagcagcacg
    17761 cgggtaattg cgtccacatc cagccagcag gcgatgacca ccgccaacaa taccgctacg
    17821 ccttcctgac ggaatgccgc ttcgttgatc catgcagcgc gtaaaccttt ccaggaatag
    17881 ccagcagctt tgataattcg ggtgaatcca gtggtattat tggccattga aagaaccttt
    17941 ttacattatg agcgtcaata tcagtgtacc gttaaatccg atgattcggg tttgcgtaac
    18001 gccgcgcgaa acatgagcgg ataccacaga atttcccatg actttctgct atccttgccg
    18061 cgcatttgca ttattaacca gaggctttac atcgtttatg tccggctggc cacgaattta
    18121 ctacaaatta ctgaatttac cattaagcat cctggtaaaa agcaagtcta ttccggcaga
    18181 tcctgccccg gaactggggc tggatacctc tcgtccaatt atgtacgttt taccgtacaa
    18241 ctcgaaagca gatttgctga cgttacgcgc ccagtgtctg gctcatgact tgcctgaccc
    18301 gttagagccg ctagaaatcg acggcacgct actgccgcgc tatgtgttca ttcacggcgg
    18361 gccgcgtgta ttcacctatt acacgccgaa agaagagtct attaagctgt tccacgacta
    18421 tctcgatttg caccgtagca acccaaatct ggatgtgcag atggtgccag tatcggtgat
    18481 gtttggtcgc gcgccggggc gtgaaaaagg cgaagtaaac ccgccgctgc gtatgcttaa
    18541 cggcgtacag aaatttttcg ccgtactgtg gctcggtcgc gacagttttg tgcgtttctc
    18601 gccgtcagtt tcgctgcgcc gtatggcgga tgaacacggc acggataaaa ctatcgcaca
    18661 gaaactggcg cgcgtggcgc gtatgcactt tgcccgtcaa cgtctggctg ccgtaggccc
    18721 acgtctgcct gctcgtcagg atctgtttaa taagctgctc gcctcccgcg ccattgccaa
    18781 agcggtagaa gatgaagcgc gcagcaaaaa aatctcccat gaaaaagcgc agcagaacgc
    18841 gattgcgctg atggaagaga ttgcggcgaa tttctcttac gagatgatcc gcctgactga
    18901 ccgcattctg ggcttcacct ggaaccgact ttatcagggc atcaacgtcc ataacgccga
    18961 gcgcgttcgc cagctggccc acgacggtca tgagctggtg tatgtgcctt gccaccgcag
    19021 tcacatggac tacctgctac tttcttacgt gctgtaccac caggggctgg tgccgccgca
    19081 tatcgccgcc gggatcaacc tgaacttctg gccggccggg ccgattttcc gccgtctggg
    19141 ggcgttcttt attcgccgta cttttaaagg caataaactt tattccaccg ttttccgtga
    19201 gtatctcggc gaactgttca gccgtggtta ttccgtcgag tacttcgtgg aaggcggtcg
    19261 ttcccgcacg gggcgtttgc tggatccgaa aaccggtacg ctgtcgatga ccattcaggc
    19321 gatgctgcgt ggcggcacgc gtccgattac gctgattccg atctatatcg gttatgagca
    19381 cgtcatggaa gtgggtactt acgccaaaga actgcgcggc gcgacgaaag agaaagagag
    19441 cctgccgcag atgctgcgcg gtttaagcaa gctgcgtaat ctcggtcagg gttacgtcaa
    19501 cttcggtgaa ccaatgccgt tgatgaccta ccttaaccag cacgtaccag actggcgtga
    19561 atctatcgat cccatcgaag cggtgcgtcc ggcctggtta acgccgacgg tcaataatat
    19621 tgctgccgat ctgatggtac gcattaacaa cgcaggcgcg gcaaacgcca tgaacctgtg
    19681 ttgtactgcg cttctggcat cacgtcagcg ctcactcacc cgcgagcagt taaccgagca
    19741 actcaactgc tacctggatc tgatgcgcaa cgtaccttac tccacggact ctaccgttcc
    19801 ttcagccagc gccagcgagc ttatcgatca cgcgctgcaa atgaacaagt ttgaagtcga
    19861 gaaagacact atcggcgaca tcatcattct gccgcgcgag caagcggtgc tgatgaccta
    19921 ctatcgcaac aacattgcgc atatgttggt gctgccttcg ctgatggcgg caattgtcac
    19981 ccagcatcgc cacatctccc gcgacgtatt gatggagcac gtcaatgtgc tttacccaat
    20041 gctgaaagcg gagctgttcc tgcgctggga tcgcgacgag ttgccggacg ttattgatgc
    20101 gctggcaaat gagatgcaac gtcaggggct gattaccctg caagatgatg agttgcatat
    20161 caacccggcg cattctcgcc ccctacagct gctggccgca ggcgcgcgcg aaacgctgca
    20221 acgttatgcc atcaccttct ggttgttgag tgccaacccg tcgatcaacc gcggtacgct
    20281 ggagaaagag agccgcaccg ttgcgcaacg tctctccgtg ctgcacggca tcaacgcgcc
    20341 ggagttcttc gacaaggcgg tgttcagttc tctggtgctg acgctgcgtg atgaagggta
    20401 tatcagcgat agcggcgacg ccgaaccggc agaaacgatg aaggtttatc agttgctggc
    20461 ggagttgatt acatcagacg tgcgtttgac gattgagagt gcgacgcagg gcgaagggta
    20521 atcagagaga attgccggat gcggcgaaaa cgccttatcc ggccttccat aatctgcaaa
    20581 ttcaataaat tgcgatgtgt tgtgtaggcc tgagaagcgc agcgcatcag gcaacccaga
    20641 agaaagccgg atgatcatcc ggctttttta catcatcaga aatgccagta actcattgcc
    20701 agtcctaaaa acagtaccag accaacatag ttattattca taaatgcttt aaagcaggct
    20761 tcacgctcgc ggttggcaat cagtttttgt tgataaacaa acagcgcgcc agccaccaga
    20821 attgaccagt aatatcccca gcctaagcca tttaactcac cgatgatcgc catcagtgcc
    20881 agtacgccaa tctgcaaaat accaataatc aatttatcgt attggccgaa caggattgcg
    20941 gtggatttaa taccaatctt cacatcatcg tcacggtcaa ccattgcata ctgcgtgtcg
    21001 taagccaccg cccagagaat attggcgagg aacattaacc agcaactcaa tggcaccgac
    21061 tcactcacag cggcaaaagc cattggaatc gaccagccaa acgccgcgcc cagcaccact
    21121 tgcgggagat gggtataccg cttcataaac gggtacaccc acgccaacgc cagagcggca
    21181 atcgacaaca gaatggtcat cgtattcagc gtcagcacca gtaaaaatga aatcaatacc
    21241 agcactacaa acagcgcgcg cgcctctttc tccgttaccg cgccgctggg aagcggtcgg
    21301 ttcgccgtgc gcttaacatg accatcaaac ttgcggtcag cataatcatt caccacacat
    21361 ccggcagcgc gcatcagcca gacacccgcg acaaacaccg ccaggatcca gagctgggga
    21421 acgcccggtg tcgccaccca caacgcccat aatgttggcc agagcagcag taacgcgcca
    21481 attggcttat ccgtacgcat taagcgatga aacgccagca gcttattctg cgtcagactc
    21541 cactccatat tttttcctct tagtacaacg gtgacgccgg taaaaacagt tctgttagca
    21601 acagcggttt accgcttaat cgcaggcggg aacgtcgccc ccacagtccg gcatcacggc
    21661 ctatctcaat aaagtcccgg gttaatgtcg atgatgtgaa cagatagcgt cctagcggcg
    21721 ttttacccaa tttttgtaac gccagctccg gcccgcttaa cgttgacaca ggaacgacgg
    21781 tacgaccggc aagccacggt tcaccatcgg cacataacaa aatttcacgt aaccagtaac
    21841 gagactcttt cggcagcagc ggcagttctt cggggatttc attctgctcg acaaaccctt
    21901 cgcggatcat cgtcacgctc accgtttttc cctgctgttc aaaacgtttt gtcatggaat
    21961 cttccagcaa cagccagtcg agcagttgcg aatccagggc agggatctct ttaaaatagc
    22021 gcagcgcacg cagttgcgtt aacgcggggt gtgacatgcc gaactctccg ttacataacg
    22081 taaaggcatt gtatcgcaga aaagtgttta cgggagatgt aaagcaaatt aagtgatcta
    22141 atgcgcaaca aaaacgcaac aatcgcatga agtggtaaaa cgctgttgaa ccttacgctt
    22201 tgccgatgcc cgtgctggcg agggcaaccg taggggcatc accgcccatg atgccaggaa
    22261 cggtgatgtt ttcctttact taaataccta tattctttat acgagtagct cgccaactcg
    22321 cagtaaaaat tgggaaaagc gcatatttac catgagattt ggagctaaaa agtgtttcct
    22381 gagtaagatt aataggcaac atcttatgtc tggatttata gatacacgtg gaattccaat
    22441 tggtatcttt tgtaggattc aacatat
//
LOCUS       DAJTZL010000055        22233 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.055, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000055 DAJTZL010000000
VERSION     DAJTZL010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22233
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.055"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            73..1461
                     /locus_tag="OO544_001840"
     CDS             73..1461
                     /locus_tag="OO544_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HCR6767451.1"
                     /translation="MSAYLRYIVQKADSSFLYDKYQNQSIAAHVMRALAAEQSEVSPE
                     QRRAICEAFESANNTHGLNLTAHKYPGLRGTLQTASTDCDTIVEAAALLPAFDQAVEG
                     NRHQDDYGSGLGMAEEKFHYYLDLNDRYVYFYEPVNVEYFAMNNWSFLQSGSIGIDRK
                     DIEKVFTGRTVLSSIYQDQRTKQNVMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTH
                     DRPLLWRFLNVTLTDTDSGRDIIINQSEDNLFQYVSYVHDLPGGIRVSLSIDILYFIT
                     SSWKSVLFWILTALILLNMVRMHFRLYQNVSRENISDAMTGLYNRKILTPELEQRLQK
                     LVQSGSSVMFIAIDMDKLKQINDTLGHQEGDLAITLLAQAIKQSIRKSDYAIRLGGDE
                     FCIILVDSTPQIAAQLPERIEKRLQHIAPQKEIGFSSGIYAMKENDTLHDAYKASDER
                     LYVNKQNKNSRS"
     gene            1504..2007
                     /gene="yeaK"
                     /locus_tag="OO544_001841"
     CDS             1504..2007
                     /gene="yeaK"
                     /locus_tag="OO544_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HCR6767452.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTTDYLRIAQPEL
                     LNFRRTA"
     gene            complement(2008..2112)
                     /locus_tag="OO544_001842"
     CDS             complement(2008..2112)
                     /locus_tag="OO544_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6767453.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            2282..2728
                     /locus_tag="OO544_001843"
     CDS             2282..2728
                     /locus_tag="OO544_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HCR6767454.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(2685..3506)
                     /gene="nimR"
                     /locus_tag="OO544_001844"
     CDS             complement(2685..3506)
                     /gene="nimR"
                     /locus_tag="OO544_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HCR6767455.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVEHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RTMVEMMAKGPVEWGSLGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            3603..4784
                     /gene="nimT"
                     /locus_tag="OO544_001845"
     CDS             3603..4784
                     /gene="nimT"
                     /locus_tag="OO544_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HCR6767456.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFIGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLATPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMIVPLA
                     LNGFGWQGALLMLMCFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGINSLIYYVIIGWLPAILISHGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGDWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            4839..5186
                     /locus_tag="OO544_001846"
     CDS             4839..5186
                     /locus_tag="OO544_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HCR6767457.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYLAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(5208..5462)
                     /gene="yoaF"
                     /locus_tag="OO544_001847"
     CDS             complement(5208..5462)
                     /gene="yoaF"
                     /locus_tag="OO544_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HCR6767458.1"
                     /translation="MKIISFALPCLLVLAGCSTSSQPEVPKPPQIGMANPASVYCQQK
                     GGTLIPVSTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            5645..6670
                     /gene="dgcP"
                     /locus_tag="OO544_001848"
     CDS             5645..6670
                     /gene="dgcP"
                     /locus_tag="OO544_001848"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HCR6767459.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGISTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLREANAALIAQSYTDSLTGLPNPRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHSAPHC"
     gene            6703..6801
                     /locus_tag="OO544_001849"
     CDS             6703..6801
                     /locus_tag="OO544_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HCR6767460.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            6804..6878
                     /gene="yoaJ"
                     /locus_tag="OO544_001850"
     CDS             6804..6878
                     /gene="yoaJ"
                     /locus_tag="OO544_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HCR6767461.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(6937..7185)
                     /locus_tag="OO544_001851"
     CDS             complement(6937..7185)
                     /locus_tag="OO544_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCR6767462.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(7333..7515)
                     /locus_tag="OO544_001852"
     CDS             complement(7333..7515)
                     /locus_tag="OO544_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HCR6767463.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYDTENEHDVCGW"
     gene            complement(7519..7878)
                     /gene="yeaR"
                     /locus_tag="OO544_001853"
     CDS             complement(7519..7878)
                     /gene="yeaR"
                     /locus_tag="OO544_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein"
                     /protein_id="HCR6767464.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGVYPRLS
                     VMHGAVKYLGYADEHSAEPDQVILIEAGQFEVFPPEKWHNIEAMTDDTYFNIDFFVAP
                     EVLMEGAQQRKVIHNGK"
     gene            complement(8051..8689)
                     /gene="leuE"
                     /locus_tag="OO544_001854"
     CDS             complement(8051..8689)
                     /gene="leuE"
                     /locus_tag="OO544_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HCR6767465.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGVATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGK
                     NNEAKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
     gene            complement(8816..9739)
                     /gene="dmlR"
                     /locus_tag="OO544_001855"
     CDS             complement(8816..9739)
                     /gene="dmlR"
                     /locus_tag="OO544_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DmlR"
                     /protein_id="HCR6767466.1"
                     /translation="MNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLE
                     QTLNVVLLHRTTRRVTITEEGERIYEWAQRILQDVGQMMDELSDVRQVPQGMLRIISS
                     FGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARK
                     LATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGHHAIKV
                     TGPLSSNHGEIVHQWCLDGQGIALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVS
                     RLATSAKVRITIEFLRQYFAEHYPNFSLEHA"
     gene            9842..10927
                     /gene="dmlA"
                     /locus_tag="OO544_001856"
     CDS             9842..10927
                     /gene="dmlA"
                     /locus_tag="OO544_001856"
                     /EC_number="1.1.1.83"
                     /EC_number="1.1.1.85"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional
                     D-malate/3-isopropylmalate/tartarate dehydrogenase"
                     /protein_id="HCR6767467.1"
                     /translation="MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFDLSFEQME
                     WASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YVNLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQES
                     VFTRRGVDRILRYAFELAQSRPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRW
                     DKQHIDILCARFVMQPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERTFP
                     SLFEPVHGSAPDIYGKNIANPIATIWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIA
                     HGPKTPDMKGSATTPQVADAICKIILR"
     gene            11178..12788
                     /gene="yeaV"
                     /locus_tag="OO544_001857"
     CDS             11178..12788
                     /gene="yeaV"
                     /locus_tag="OO544_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter YeaV"
                     /protein_id="HCR6767468.1"
                     /translation="MMSNVKKKDVPLISISLVAILFIAAALSLFPQQSADAANTIYTF
                     VTRTLGSAVQVLVLLAMGLVIYLATSKYGNIRLGEGKPEYSTLSWLFMFICAGLGSST
                     LYWGVAEWAYYYQTPGLNIAPRSQQALEFSVPYSFFHWGISTWATYTLASLIMAYHFH
                     VRKNKGLSLSGIIAAITGVRPQGPWGKLVDLMFLIATVGALTISLVVTAATFTRGLSA
                     LTGLPDNFTVQAFVILLSGGIFCLSSWIGINNGLQRLSKMVGWGAFLLPLLVLIVGPT
                     EFITNSIINAIGLTTQNFLQMSLFTDPLGDGSFTRNWTVFYWLWWISYPPGVAMFVTR
                     VSRGRKIKEVIWGLILGSTVGCWFFFGVMESYAIHQFINGVINVPQVLETLGGETAVQ
                     QVLMSLPAGKLFLAAYLGVMIIFLASHMDAVAYTMAATSTRNLQEGDDPDRGLRLFWC
                     VVITLIPLSILFTGASLETMKTTVVLTALPFLVILLVKVGGFIRWLKQDYADIPAHQV
                     EHYLPQTPVEALEKTPVLPAGTVFKGDN"
     gene            12820..13944
                     /gene="yeaW"
                     /locus_tag="OO544_001858"
     CDS             12820..13944
                     /gene="yeaW"
                     /locus_tag="OO544_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaW"
                     /protein_id="HCR6767469.1"
                     /translation="MSNLSPDFVLPENFCANPQEAWTIPARFYTDQNAFEHEKENVFA
                     KSWICVAHSSELANANDYVTREIIGESIVLVRGRDKVLRAFYNVCPHRGHQLLSGEGK
                     AKNVITCPYHAWAFKLDGNLAHARNCENVANFDSDKAQLVPVRLEEYAGFVFINMDPN
                     ATSVEDQLPGLGAKVLEACPEVHDLKLAARFTTRTPANWKNIVDNYLECYHCGPAHPG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTDEQKSLIEWYRDVFRPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            14000..14965
                     /gene="yeaX"
                     /locus_tag="OO544_001859"
     CDS             14000..14965
                     /gene="yeaX"
                     /locus_tag="OO544_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaX"
                     /protein_id="HCR6767470.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHII
                     VQMSDGDNQYSNAYSLLSSPHDTSCYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDVDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALNEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(15019..16134)
                     /gene="rnd"
                     /locus_tag="OO544_001860"
     CDS             complement(15019..16134)
                     /gene="rnd"
                     /locus_tag="OO544_001860"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HCR6767471.1"
                     /translation="MITTDDALASLCEAVRVFPAIAQDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEEALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(16216..17901)
                     /gene="fadD"
                     /locus_tag="OO544_001861"
     CDS             complement(16216..17901)
                     /gene="fadD"
                     /locus_tag="OO544_001861"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HCR6767472.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLIDMFEQSVVRYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCCRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(18106..18687)
                     /gene="yeaY"
                     /locus_tag="OO544_001862"
     CDS             complement(18106..18687)
                     /gene="yeaY"
                     /locus_tag="OO544_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HCR6767473.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MNAPDLYVGQEARFGGKVVVVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGTVDGKVNNAPYKFMVMQVTGYKRWHITQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVETIVTE"
     gene            complement(18727..19422)
                     /gene="tsaB"
                     /locus_tag="OO544_001863"
     CDS             complement(18727..19422)
                     /gene="tsaB"
                     /locus_tag="OO544_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HCR6767474.1"
                     /translation="MRILAIDTATEVCSVALWNDGNVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLAAIDARMGEVYWAEYQRDENGIWRGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLRKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(19480..21390)
                     /locus_tag="OO544_001864"
     CDS             complement(19480..21390)
                     /locus_tag="OO544_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HCR6767475.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGCAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDLLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            21522..21866
                     /locus_tag="OO544_001865"
     CDS             21522..21866
                     /locus_tag="OO544_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCR6767476.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
BASE COUNT         5442 a         5698 c         5403 g         5690 t
ORIGIN      
        1 tttatcgccg caagtgtcat tgtgctgaca tcttccttcc ttatttttga acttgtcgcc
       61 agcgacagag caatgagtgc ctatctgcgc tatatcgtgc agaaagcaga ctcctccttt
      121 ctttatgata agtatcagaa tcagagtatt gccgcgcatg tgatgcgcgc tctcgctgct
      181 gagcagtcgg aagtgtcgcc agaacagcgg cgcgccatct gcgaggcttt tgagtctgcc
      241 aataataccc atggcttaaa cctgactgcc cataaatacc cgggcttacg cggcacacta
      301 caaaccgcat ccactgactg cgacacaatt gtggaagctg cagcactatt acccgctttt
      361 gatcaggcag tggaaggcaa ccgccaccag gatgattacg gttcaggtct tgggatggcc
      421 gaagagaaat ttcactatta tctcgatctc aatgaccgct atgtctattt ttatgagccg
      481 gttaatgttg aatactttgc gatgaataac tggtccttcc tgcagtcagg aagtattggc
      541 atcgatcgca aagatattga aaaggtattt accgggcgta ccgtattgtc gagcatttac
      601 caggatcagc gtactaaaca gaacgtgatg agtttgctga cgccggtata tgtcgcaggg
      661 cagctaaaag ggattgtgct gctggatatt aacaaaaaca atttgcggaa tatcttttac
      721 actcatgacc gccctctcct ctggcgtttt ctcaatgtca cgctaaccga taccgattcg
      781 gggcgcgaca ttatcatcaa ccagagcgaa gataatctgt tccagtatgt cagttacgtc
      841 catgacttac cgggcggcat tcgtgtctcg ttatccattg atattcttta ctttatcacg
      901 tcttcgtgga aaagcgttct gttctggatt ttgacggcgt taattttgct gaatatggtc
      961 cgaatgcact tccgtttata ccaaaatgtg tcgcgagaaa atattagtga tgcgatgact
     1021 ggactgtata atcgcaaaat tttaacccct gaactggagc agcggttgca gaaactggtg
     1081 caatccggtt cttcggtgat gtttattgca attgacatgg acaagttaaa gcaaataaat
     1141 gacaccctcg gtcatcagga gggggattta gcgattacgt tattagctca ggcgattaaa
     1201 caatcgattc gtaaaagtga ttatgccatc cgactcggtg gcgatgaatt ctgcatcatt
     1261 cttgtcgatt cgacgccgca aattgcagca caactgcctg aacgtatcga aaaacgtctg
     1321 caacatatcg cgccgcagaa agaaatcggc ttctcttccg gtatttacgc gatgaaagaa
     1381 aacgatacgt tacatgatgc gtataaagct tccgatgagc gtttatatgt caataagcag
     1441 aacaaaaaca gccgttcatg ataaccttct gtggttgttt gcttgtaatc tcaggagcgt
     1501 gaaatgactg aaatggctaa aggaagcgtg acgcatcagc ggttaatcgc tttattatca
     1561 caagaaggtg ctaacttccg cgtcgttacc catgaagcgg taggtaaatg tgaagcggtg
     1621 tcagaaattc gtggcaccgc tttaggccag ggagctaagg cactggtatg taaagtcaaa
     1681 ggcaatggcg taaatcaaca tgttctggcg atcctcgctg ccgatcaaca ggccgatctg
     1741 agccaacttg ccagccatat aggcggatta cgcgcttcgt tagccagccc tgctgaagtc
     1801 gatgaactca caggctgtgt cttcggcgcg atcccccctt tcagcttcca tccaaaactc
     1861 aaactggttg ccgaccctct actcttcgaa cgatttgatg aaatcgcctt caatgcaggg
     1921 atgctggata aatccgttat tttgaaaacc acggattatt tgcgcattgc gcaaccagaa
     1981 ctgctcaatt tccgccgcac tgcgtaacta gcctgtccgt tcaataagca gaacggacaa
     2041 aaccagcaca acagcaatgg caaaaaacga tgacgtgata atcagtgtct cgacgaacat
     2101 ttgatcgttc atagcatgcc cccgggagat agctgttttc acgttattgt tagcgtgcac
     2161 aaatggcagt ttgatgacaa ttcgcctgat tttttatgca aaaaacgtaa agattttcta
     2221 cttccttcct gcagcaaacg taaagtaagc aggcttatta ttttttggca aggaaaccac
     2281 gatgtttgat gtcactttgc tgatcctgct cgggttagct gcgctgggct ttatcagtca
     2341 taacaccact gtcgccgttt caattctggt gttgatcatt gtccgcgtca caccgttaag
     2401 cacctttttc ccgtggattg aaaaacaggg gctaagtatc gggataatca ttctgacgat
     2461 tggcgttatg gcccccatcg ccagcgggac gctaccgccc tcaaccttga ttcactcgtt
     2521 tctgaactgg aaatcgctgg tggcaattgc agtaggcgtg attgtctcct ggctgggtgg
     2581 gcgcggcgtg accttaatgg gcagccagcc gcaactggtc gccgggctgc tggtaggcac
     2641 tgttttaggc gtggcgctgt ttcgcggcgt accggtcggc ccgcttattg ccgcgggtct
     2701 ggtttcgctg attgtgggga aacagtagtt aatcccgcaa tatatcggcc tggcgtttgc
     2761 cccaggcctt tcttaaacat agtaataaag gccgttgttg aatcataccc cagcgtatga
     2821 gcaactttct gtacagtatc gcctttcact aatccctgaa gtgccataat cagctgtaac
     2881 tgctgacgcc actggcgaaa actcaacccc gtctctttta ctatcagtcg cgcaagattg
     2941 cgttcactca tcgcaaaaaa gccagcccat tgccctaatg acccccactc gacgggccct
     3001 ttcgccatca tctccaccat cgtgcggatt ttaggatgag aagaaacggg caggtgcagc
     3061 tgttgttgtg gctgctgagg gagttcatca aacaggacct gaattaatct gcgggtcatc
     3121 ggctcagcac gctgagtcgt cgtcctgttt gccagggtta aaatcaattc ccggcataag
     3181 ggcgatattt tcaatgtgca acatgttgtc ggcattgtca ctgcgctggg ttcgataaac
     3241 agaaaacaga gttcagcgtt agctgtcact tgattgctat gttccacccc gcccggtatc
     3301 caaaccgcat attgcggcgg caccatccat aaagcatttt ccaccgtaca ggtaattgca
     3361 ccatgtaatg ctagtatcag ctgtcctttg cggtgctgat gtacaggact ggttaactca
     3421 tccgttccgg cgtgaataca aaatgcgacc gcggcttcat ggtgtcggtc gggttcatag
     3481 ccattgagat tcaacctgtg catcattttg tccgaattta gcgataattt gtcattttag
     3541 cttgattcaa cataacaata aaaacggtaa ggtacagcct cgtttgtaac aatgagaagc
     3601 atatgacctg ttcaacttca ttaagcggca aaaacaggat tgtccttatc gctggcattc
     3661 tgatgattgc cacaacatta cgcgtcacct ttatcggcgc agcaccgtta ctggatacga
     3721 ttcgttccgc ttactcgctg acgacagcgc aaaccggctt attgaccacc ctgccattat
     3781 tggcctttgc gctaatctca cctttggcca ccccggtagc gcgacgtttt ggtatggaac
     3841 gtagcctgtt tgccgcgtta cttttgatct gtgctggtat cgcaattcgc tctctccctt
     3901 cgccttactt attatttggc ggtacagcgg tcattggcgg tgggattgca ttaggcaatg
     3961 tcttactgcc aggattaatt aaacgcgatt tccctcattc cgtcgccaga cttaccggcg
     4021 catattccct gacaatggga gctgcagcgg cactgggatc ggctatgata gtgccgctgg
     4081 ctttgaacgg ttttggctgg caaggcgcgt tgctcatgct gatgtgtttt cctctgctgg
     4141 ctcttttttt atggctgcca cagtggcgaa gccaacaaca tgcaaatttg agtacctcgc
     4201 gcgccttaca tactcggggt atctggcgtt cgccgcttgc ctggcaggta acattgtttc
     4261 ttgggatcaa ctcactgatc tattacgtga ttattggctg gcttccggcg atcctcatca
     4321 gtcacggcta tagcgaagca caggcgggtt cactgcatgg tttgctgcaa ctagccacag
     4381 cagctcccgg tttgctgatc cctcttttct tacatcatgt gaaagatcag cgtggtattg
     4441 cagcgttcgt tgccttgatg tgcgcagtgg gcgctgttgg gctctgcttt atgccagcgc
     4501 acgcgatcac ctggactctg cttttcggtt ttggttccgg cgcaacaatg atactggggt
     4561 tgacgttcat tggtcttcgg gcaagttctg cgcatcaggc ggcggcactc tcggggatgg
     4621 cacaatccgt cgggtatttg ctggcagcct gtgggccgcc gctaatgggt aaaatacacg
     4681 atgctaacgg tgactggtcc gtaccactga tgggtgttgc aatactttca ctactgatgg
     4741 cgattttcgg actttgcgcc gggagagaca aagaaattcg ctaatatccg gtgctatagt
     4801 gacgtgacaa atcatgcgtg aaagggagaa caaacactat gaatattcag tgcaaacgcg
     4861 tttatgatcc ggctgaacag agcgatggtt atcgcgtact ggtcgaccgc ctctggccgc
     4921 gcggtatcaa aaaaaccgat ttagcccttg atgagtggga taaagaaatc acgccgtcaa
     4981 cggaactgcg caaagccttt cacggcgaag tcgtcgattt tgcaaccttt cgcgagcaat
     5041 atcttgcaga actggcgcaa cacgagcaag aaggaaagcg gctggcggac atcgccaaaa
     5101 aacagccgct gaccctgctc tacgcagcaa aaaacaccac gcagaaccat gcgctggtgc
     5161 tggccgactg gctacgtagc ttgtgatttt agtacagcat ccggcggtta tttttcacca
     5221 gccggatggt cccgtcgcca caatgcccat tcatcaatcg tttcaccgcc cggtaatttg
     5281 caattgttgc tgaccccttg cgctgtcgac actggaatga gcgtcccgcc cttctgctgg
     5341 caatagaccg acgccggatt tgccatacca atctgcggcg gtttaggtac ttctggctga
     5401 gaagaggttg aacaaccagc caggaccagc aagcaaggca aagcaaaact gataattttc
     5461 atttattgat ctcactcatt tatctacgct tagagtatcg cggtatcgtt ttgttttgca
     5521 gcactatttt tattacattc actcaaaaca tattacgtct tgtttcatct ttgttggtga
     5581 tgttttatca tgcctgcaaa gattaaataa tcagcattta cccgccgtat cctggagttg
     5641 tttcgtgtca gatcagatta tcgcccgcgt ctcgcaatcc cttgccaaag aacagtcact
     5701 ggaaagtctg gtccgacagc ttctggagat gctggaaatg gtcactgata tggaatcaac
     5761 ctacctgacc aaagtggatg tcgaagcgcg cctgcagcat attatgtttg cccgtaacag
     5821 ccagaaaatg cacatcccgg agaattttac cgtctcgtgg gattactcgt tatgcaaacg
     5881 cgccattgat gaaaactgct ttttcagcga tgaagtccct gaccgttggg gcgactgtat
     5941 tgcggcacgc aatcttggca tctccacatt tctgagcaca ccaattcact taccggatgg
     6001 atcattctat ggcacgcttt gcgccgccag cagtgagaag cgccagtgga gtgaacgcgc
     6061 ggaacaggtt ttacagttat tcgccggact gattgcacaa tatattcaaa aagaggcact
     6121 ggttgaacag ttgcgcgaag ccaatgctgc gctgattgcg caatcgtata ccgactcgtt
     6181 aaccgggttg ccgaatccgc gggcgatttt tgaaaatctg acgacgctgt tttctctcgc
     6241 ccggcatctt aaccataaga taatgatcgc gtttatcgat ctggataact tcaaattaat
     6301 caatgatcgt tttggtcata atagtggcga tctgtttctc attcaggttg gcgagcgcct
     6361 taatacgctc cagcaaaatg gcgaagttat tggtcgtctc ggcggtgatg agtttttggt
     6421 tgtttcacta aacaacgaga atgcggatat ttcgtcgctg cgagaacgca ttcagcagca
     6481 aatacgtgga gaatatcact taggtgatgt tgatttgtat tatcccggtg ccagtcttgg
     6541 catagtagaa gtcgatcctg aaacaaccga tgcagacagt gccctgcatg ctgccgatat
     6601 tgcgatgtat caggagaaaa aacacaaaca gaaaacacct tttgtcgcgc attcagcgcc
     6661 acattgctga ggcgtattca catccttttg attggtgata acatgcgaat cggtattatt
     6721 tttccggttg taatctttat tacagcggtc gtatttttag catggttttt tattggcggc
     6781 tatgctgccc cgggggcata aagatgaaaa aaacaacgat tattatgatg ggtgtggcga
     6841 ttattgtcgt actcggcact gagctgggat ggtggtaacg tcacctctaa aaaatagcaa
     6901 aggctgcctg tgtgcagcct ttgtgcaatt taagcgttaa cttttaatct tcctgtagat
     6961 aaatagcacg acaatcgcac caataacggc aaccacgaag ctgccaaaat tgaagccatc
     7021 gactttacca aagccaaaca gcgtgctgat ccatccgccg actacggcac cgactatccc
     7081 cagcaggata gtcataaaga atccaccacc atctttacct ggcatgatcc acttcgccag
     7141 aataccggca ataagcccaa aaataatcca tgacagaatg cccattgttt cctcacttat
     7201 ctgttttgca ttagcgggtt agtcgctgat aaaaagcata gcacaacatc gggagggcaa
     7261 gatttgtgac gagcatcacg gaggattttt ttgcgatggc gcagaaattg cgccatcaac
     7321 gatcagtgat aattaccaac cacaaacatc atgttcgttt tccgtgtcat aagaacgcac
     7381 ggtattcacc agatctttta tcacttcagc cgccacttct ggcaccagca aagtcatcgg
     7441 cgtctctgtt tcataatcga cagaaacgcc attactgtta ttggtgacgg tcacggtata
     7501 cgttgctttg cccatgattc atttcccgtt atgaatgact ttccgttgtt gcgcaccttc
     7561 catcaggact tcaggagcca cgaagaagtc aatgttgaaa taagtatcgt cagtcatggc
     7621 ttcaatgttg tgccactttt ctggagggaa cacctcaaac tgccccgctt cgataaggat
     7681 cacctgatca ggctctgcac tgtgttcatc agcgtagccg agatatttga ccgccccatg
     7741 cataacggaa aggcgtgggt aaacccccgg gcgcgttcct ttatcaagat gacgttcgaa
     7801 tattccggca ggcgcagttt gtttattcca gaaaggcgtt gagcgcgtat gaatataatt
     7861 ctgtgggatt tgcagcatcc ttttccctcc ttcggtgaat gcgctgaaaa cggtttattc
     7921 cagccgtttc agggtacgcc tgataatttg cattttaaat accatttatt ggttactttt
     7981 tagcaccata tcagagaaaa atcagggagg attacaagtg ggaagcccgc gacaacgcgg
     8041 gctgaatgta tcaggattgc agcgtcgcca gtcgggcggc gaaacccacg aacatcaaac
     8101 caatcagtga gttgccaact ttagccagtt tctttttggt acgtatgtac tgcgtgacaa
     8161 aagcaccaga aataatcagg aagctcaaat agcagaaact caccagttcc agcgtcgtcg
     8221 ccagaataaa gaatgaaatt cccgtatgtg gggcattaac atcgataaac tgtacgaaaa
     8281 acgacacata gaacaaaatg gctttcggat tagtcaggct caaaattaac gcgcgtttaa
     8341 aaatggcccc gtattggggc tcatcggatt tggcctcgtt atttttaccc ttcagggtcg
     8401 cgtagagaat tttactcccc agatagagca aataaaacgc accaagataa cgtacaatgt
     8461 tgaataatat cggggtggtc ttaattaatg tcgccactcc agcccatgcc agaaacatca
     8521 atactgcatc gccaataaat acaccgcagg ccgcaagata accgcctttc ataccgctac
     8581 tgacgctatt tttgagtaca aacagggtat ttggccctgg caccaacaca ataaaaatgg
     8641 ccccaaccag ataggtccag taattcagaa ccccgtattc agcgaacaca ttaacctctt
     8701 taattatctt tcgatcatgc gcgattaaag gtgaatatgc taaccaatct gtagcggctt
     8761 agaaaggaga aaatcaggtt ttaacccgat atcaacccga taattgaata attaatcagg
     8821 catgctccag tgaaaaattc gggtagtgct cggcaaaata ctggcgtaaa aattctatcg
     8881 ttatccgcac tttcgctgac gtcgccagcc ttgaaacata aacggcccag acgttcgctg
     8941 gctggtaata ttccggtagc acttgcacta aatgaccact ggcaatgttt tcgctaacat
     9001 cccaccagga gcgcagagca atcccctgcc catcgagaca ccactgatgg acaatttctc
     9061 catgattaga cgacagtggg ccggtgactt taatcgcatg gtggccttct ttattgcgta
     9121 attgccagac accaaaagga tgatcgcgct ctttaatgac caggcacggc aatgcggaaa
     9181 gatcggttaa atgtttcggc gcgccatgtt gcgcaataaa ttccggcgac gcgcagagaa
     9241 tgcggtaatt agtcgccagt ttgcgggcga ttaaattagg ggcgatgtca tcgccaatgc
     9301 gaatatcgag atcaacacct tcattgacca aatcgaccaa ccggtcttcc acatcaaaac
     9361 gtaattccag ttgcggatac gccttagcca gcgctgataa cgccggagcc accacctgtc
     9421 gtccaaaacc aaagctgctg ataatacgca gcatcccctg cggcacctga cgcacgtcag
     9481 aaagttcgtc catcatctga ccgacatcct gcaaaatccg ctgcgcccat tcataaatcc
     9541 gctctccttc ttcggtaatg gtgacgcggc gggtggtgcg gtgtaacaac accacgttta
     9601 gcgtttgctc cagcaaggcg atgcgcttgc tgacgaacgc cggtgaaacg cccagttctt
     9661 ccgccacggc ggcaaaacca gcccggcgag ccaccagcat aaagacgcgc aaatcgttca
     9721 gcagcggtaa attattcatg attcgtgttt tatgtttcac cagttacggg gattaattcc
     9781 ttttcagtca attataggat ggtgatgttg tcaattttga tggtcaggaa gtgagaaccc
     9841 aatgatgaaa acgatgcgta ttgctgcgat cccgggagat gggattggca aagaagtcct
     9901 tcctgaaggg attcgcgtat tacaggctgc cgctgagcgc tggggcttcg acttgagttt
     9961 tgagcaaatg gagtgggcga gctgcgagta ttacagccat cacggtaaaa tgatgccgga
    10021 cgactggcat gagcaactta gccgtttcga cgccatctat tttggtgccg tcggctggcc
    10081 ggataccgtt ccggaccata tttcgttgtg gggttcgctg ctgaaatttc gtcgtgaatt
    10141 cgaccagtac gtcaacctgc gcccggttcg tctctttcct ggcgttccct gcccgctggc
    10201 gggaaaacag cctggcgaca tcgattttta cgtggtcagg gaaaacaccg aaggcgaata
    10261 ttcctcgctc ggcggtagag tgaatgaagg tacagagcat gaagtcgtca ttcaggaatc
    10321 ggtatttacg cgtcgtggtg tcgatcgcat tttgcgttat gccttcgaac ttgcgcaaag
    10381 ccgcccacgt aagacgctaa cttctgccac taaatcaaac ggtttagcca tcagcatgcc
    10441 gtactgggat gagcgagtgg aagcaatggc cgagaattac ccggagatcc gctgggacaa
    10501 gcagcatatt gatattctct gcgcgcgttt tgtgatgcag ccggaacgct tcgatgtggt
    10561 ggtggcgtcc aatttgtttg gcgatatcct ttccgatctt ggcccggcct gcaccggcac
    10621 cattggcatt gccccatccg ccaacctgaa tccggaacgc actttcccgt cgctcttcga
    10681 gcctgtccac ggttccgcgc cggatatcta cgggaaaaat attgctaacc ctatcgccac
    10741 aatttgggcc ggtgcgatga tgctcgattt tctcggcaat ggcgatgagc gtttccagca
    10801 agcgcataac ggtattctgg cagcgattga agaagtgatt gctcacgggc cgaaaacacc
    10861 ggatatgaaa ggcagtgcca ccacgccaca ggttgccgac gcgatttgca aaattatttt
    10921 gcgttaaggt caaaccagtt tatttgaacc gcgtcactga cgcggttttt ttattcgttc
    10981 tttgcagtta ataacctgcg tcatttcacc ttttattgtt tccgtttcgt gttttatggc
    11041 tttccgtatt cttaattgtt taatttatgt aacatgcaaa taatcaattt gttaaacaca
    11101 acatacaact cattaccctg caaataatta caactggcaa aagccagaga cgggctttta
    11161 tgcccaggag atgactcatg atgagcaatg ttaagaaaaa agatgtgccg ctgataagca
    11221 tcagcctggt ggccattctt ttcatcgcag ctgcattaag ccttttccca caacaatcgg
    11281 ccgacgcggc caacacaata tacacttttg ttacgcgtac gttaggttcc gccgtacagg
    11341 tattggtttt gctggcaatg ggactggtga tttatttagc caccagtaaa tacggcaata
    11401 ttcgtcttgg cgaaggaaaa ccggaataca gcacgctctc ctggctgttt atgtttattt
    11461 gtgccggttt aggttcttct acgctttatt ggggggttgc tgaatgggcc tattattatc
    11521 aaacgcctgg attaaatatc gcaccacgtt cacaacaggc actcgaattt agcgtccctt
    11581 actccttctt ccactggggc atcagcacct gggcaactta tacgctggcc tcattaatca
    11641 tggcttatca ctttcatgtg cgtaaaaaca aaggtctgag tctttccggc attattgccg
    11701 cgattaccgg cgttcgcccg caaggcccat ggggaaaact ggttgatttg atgtttctga
    11761 tcgccactgt cggcgcactg actatttccc ttgttgtcac cgcagcaacc tttactcgtg
    11821 gactttccgc gctgaccggt ttacccgata acttcaccgt gcaggcattt gtgatcctgc
    11881 tttccggcgg cattttttgc ctaagctcgt ggattggtat caacaacggt ttgcaacgtc
    11941 tgagcaaaat ggttggctgg ggcgcgttcc tgctgccatt actggtgctg attgtcggtc
    12001 caaccgaatt tattaccaac agcatcatca atgccatcgg cctgaccacg caaaacttcc
    12061 tgcaaatgag cttattcacc gatccgcttg gcgatggttc atttacccgc aactggaccg
    12121 ttttctactg gctgtggtgg atctcatacc cccctggcgt agcaatgttt gtcacccgcg
    12181 tttcccgcgg tcgtaagatt aaagaagtta tctggggact gatcctcggc agcaccgtcg
    12241 gttgctggtt cttcttcggc gtaatggaaa gctatgccat tcatcagttt atcaatggcg
    12301 taatcaacgt cccacaggtg ctggaaacac tgggcggcga aacagctgta cagcaagttc
    12361 tgatgtcgtt gccagccggt aaattgttcc tcgccgcata cctgggcgtg atgattattt
    12421 tccttgcctc gcatatggat gcagtggcct acaccatggc ggcgaccagt acgcgtaatc
    12481 tccaggaagg tgacgatcct gaccgtgggc tgcgtctttt ctggtgcgtg gtgatcactc
    12541 tgatcccgct ttccatcttg tttaccggtg cttcgctgga aacgatgaaa accaccgtcg
    12601 tgctcacagc ccttcccttc ctcgtcattt tactggtgaa agtcggcgga tttattcgct
    12661 ggctgaaaca ggattatgcc gacattccgg ctcatcaagt tgaacattat ctcccgcaga
    12721 caccggttga agccctggaa aaaacaccag tgctccctgc gggaaccgta ttcaaaggcg
    12781 acaactgagc gccatcatcc taacgataaa ggtatcccta tgagcaatct gagccctgac
    12841 tttgtactac ccgaaaattt ttgcgctaac ccgcaagagg cgtggaccat tcctgcccgt
    12901 ttttataccg atcagaacgc gtttgaacac gaaaaagaga acgtcttcgc caaaagctgg
    12961 atttgcgtcg ctcacagcag cgaactggcg aatgccaatg attatgtgac gcgtgagatc
    13021 attggcgaaa gcatcgtgct ggtacgcggt cgtgataagg ttttgcgcgc gttctataac
    13081 gtgtgtccgc accgtggtca tcagttgttg agcggtgaag gaaaagcaaa aaatgtgatt
    13141 acctgcccat atcacgcatg ggcattcaaa ctcgatggca atctggccca tgcacgtaac
    13201 tgcgaaaacg tcgccaattt cgatagcgac aaagcgcaac tggttccggt gcgtctggag
    13261 gaatatgccg gattcgtctt catcaacatg gaccccaacg ccaccagcgt agaagatcag
    13321 ttacccggtc tgggcgcgaa agtgctggaa gcctgcccgg aagtccacga tctgaaactg
    13381 gcggcccgct ttaccacccg cacgcctgcc aactggaaga acattgtcga taactatctc
    13441 gagtgctatc actgtggtcc ggcgcatcca ggtttctccg actccgtaca ggttgatcgc
    13501 tactggcaca ccatgcacgg taactggacg ctgcaatacg gtttcgccaa accgtccgaa
    13561 cagtcgttta aatttgaaga gggtacggat gcggcattcc acggtttctg gctgtggccg
    13621 tgcacgatgc tgaacgtcac cccgatcaaa gggatgatga cggtcattta tgaattcccg
    13681 gtggattctg aaactaccct gcaaaactac gatatttact tcaccaatga agagttaacc
    13741 gacgagcaaa aatcgctgat tgagtggtat cgcgatgtgt tccgtccgga agatttacgt
    13801 ctggttgaaa gcgtacagaa agggctgaaa tcgcgtggct atcgtggtca ggggcgcatc
    13861 atggccgaca gtagcggtag tggcatttcc gaacatggta tcgcccattt ccataatctg
    13921 ctggcgcagg tgtttaagga ctaatgacat cggcggcggt attttccgcc gctgggctga
    13981 tttttgatgg agtacagcaa tgtcagacta tcaaatgttt gaagtacagg tgagtcaggt
    14041 ggaaccgctt accgaacagg tgaaacgctt cacgctggtg gcaaccgatg gcaaaccgct
    14101 gcctgcgttc accggaggaa gtcacatcat tgtgcagatg agcgatggtg ataaccagta
    14161 cagcaatgcg tattcactac tgagttcgcc gcatgacacc tcttgttatc agattgccgt
    14221 tcggctggag gaaaactcgc gcggcggttc ccgctttttg catcagcagg taaaagtggg
    14281 cgatcggtta acgatttcaa cgcctaataa cctgtttgcg ctaattccct cagccagaaa
    14341 gcatctgttt atcgcgggcg gtattggtat cacccctttc ctgtcgcaca tggcagagtt
    14401 gcaacacagc gacgtcgact ggcagctaca ttactgctcg cgaaatccag aaagttgcgc
    14461 atttcgtgat gagctagtcc agcatccgca ggctgagaaa gtccatttgc atcattcgtc
    14521 aaccggaaca cgactggaat tagcgcgatt attggcggat atcgaacctg gcacacacgt
    14581 ttatacctgt ggccccgagg cgctaaatga agcggtaaga agtgaagctg cgcgtctgga
    14641 catcgccgcc gatacgctgc actttgagca atttgctatc gaagacaaaa ccggcgatgc
    14701 ctttaccctg gtgcttgccc gttccggaaa agagtttgtg gtgccggaag agatgactat
    14761 tttgcaggtt attgaaaata ataaagccgc gaaagtggaa tgtttatgtc gtgaaggggt
    14821 atgcggaacc tgcgaaacag caatactgga aggtgaagct gaccatcggg atcagtattt
    14881 tagcgatgaa gagcgtgcca gccagcaaag tatgttgatc tgttgttcgc gtgcgaaggg
    14941 taaacgcctg gtgttggatt tgtagtttgc caaagccgga tgtggcgctg agcgcgccca
    15001 gtccggcttc ggaagatttt actgcggata ttcctgcaat aaattgtgta atgcttccgc
    15061 catcagctca ccacgccagc cggaaatcag ctccggcaaa ttgttctgcg gtttcagttt
    15121 ccagtgccag ttcagcagtt ggttgatttg ccgacgcgat gccagcaatt cggcgctgat
    15181 cttatgcgtt tcgctcacgt cagtaatcag cgacttaatc gctttaaacg ctttacgata
    15241 acccggcatg tccatcaggt taagcatcgg ctgcggtaag gcctcttccg gcaatgtctg
    15301 cgctttttcc accagcgcca gcagcgtttt accgtgaaag cggatttcgc taccggataa
    15361 acccaggctg tccagttcgc ctaaacttcc cggcatataa cgcgcgaccg accacaaatg
    15421 ctcttcacgc acgacaaagt tcaccgccag atcacgctca cgagccttgc gcagtcgcca
    15481 gtcggctaac agttgcagac aggccagttg gcgtgtgcgt aattgccagg cattggtgat
    15541 atcacgccag gcatcttccg gcgcaacgac ttcctgacga cgcatttgca tcaggcggca
    15601 ttcatccagc gccgcaggta gccagccgga ggcctccgtt tctaccataa gcttggcggt
    15661 gatcggtaac agataccaga catccgccgc tgcgtattca cactgacgtt cggtcagcgg
    15721 tctggccagc cagtcggtgc gcgattcact cttgtccagc gtaacgccgg aatactcttc
    15781 caccatggaa gcgaaacccc atgacatcgg gcgtccgcag aaggcagcaa ggatttgcgt
    15841 gtcaatcagg ggttgtggta attcgccaaa gacattgagg aacacttcca gatcttcact
    15901 gcctgcatgg agaaattttg tgatggacgg atcgcgcagg atcgctttca gcggtgacca
    15961 gtcggtgatc ccgagaggat caattagcgc cagatgctca ccatcgaaaa gttgaatcaa
    16021 ccccagctgc gggtaataag tgcgcgtacg aacaaattca gtatcctggg ctatcgccgg
    16081 aaagacacgg acggcttcac acaaagaagc cagcgcatcg tccgtggtaa tcatttggta
    16141 attcaaattg tattctcttt agtgggcgtc aaaaaaaacg ccggattaac cggcgtctga
    16201 cgactgactt aacgctcagg ctttattgtc cactttgccg cgcgcttcgt cacgtaattc
    16261 tcgtcgcaaa atttttccga cgttagattt cggtaactca tcacgaaact ccaccagctt
    16321 cggtactttg tagcccgtga gctgacggca gcaaaaggtc accagtgact cttcggtaag
    16381 cgatggatct tttttcacta cgaagatttt caccgcttca ccactggagc cggaaggtac
    16441 gccaacagcc gcgacttcct gtacgccagg atgctgcatg acgacatctt caatctcgtt
    16501 gggatagacg ttaaaaccgg aaaccagaat catgtctttt ttacgatcga caatgcgcag
    16561 gaacccttct tcatccatca ccgcgatgtc gccggtgtgt aaccagccat ttttgatgat
    16621 ctcatcggta gcatcgggac gctgccagta acccagcatc acctgcggtc ctttgacaca
    16681 aagctcaccc ggttgacccg gtggtacttc attatcgtcg tcatccacca gtttggcttc
    16741 cgtcgacggc accggcaaac cgatgctacc actatgataa tcaatatcat atgggttaac
    16801 gctgaccagc ggcgcacact cggtaagacc atagccttcc agcagatact gtcctgtcag
    16861 tttcacccaa cgctctgcca ccacttgctg cactggcatc ccaccgcctg cggaaagatg
    16921 cagactggag aaatccagct gctggaactc tttattgttc agcaacgcat tgaacaaggt
    16981 gttaacgcca gtgatagcgg taaacggata tttcgctaac tcttttacca accctggaat
    17041 atcgcgcggg ttagtgataa gcaggttctg cccacccagt tcgataaaca gcaggcagtt
    17101 aatggtcagg gcaaaaatgt gatacagcgg cagtgccgtt actaccagtt ctttacctgg
    17161 atgcagcaac ggcccatagg tggcgttgac ctgttccagg ttcgccagca tattgcggtg
    17221 agtcagcatc gcgcctttcg ccacacctgt ggtgccgccg gtgtattgca ggaaggctaa
    17281 atcttccggc accagttcgg gtttgacgta ctgcatccgg tagccgttat gcagtgcgct
    17341 acgaaatgaa atggcatctg gcagatggta tttcggcacc aaacgcttga tgtatttaac
    17401 aacgaaattg actaccgtgc cttttgccgt agatagctga tcgcccatac gggtcagaat
    17461 tacgtgctga acggcggttt tatcaaccac tttttccagt gtgtgagcaa agttagacac
    17521 gataacaatc gccgatgcgc cgctatcgtt aagctgatgc tcaagctcac gcggggtata
    17581 caacgggtta acgtttacga cgatcatccc ggcacgcaaa atgccaaaca gcgccaccgg
    17641 atattgcaat aaattaggca tcatcaacgc aacgcgatcg cctttcttca gccccaaccc
    17701 ttgttgcaaa taagcggcaa acgcgcgact gcgttcttcc agcttgcgga aggtcattac
    17761 ctcccccata ttcacaaacg caggctgatc ggcgtagcgg acgaccgact gctcaaacat
    17821 atctatcaga gattgataac ggtcagggtt gatctccgtc ggaacatccg cgggataacg
    17881 gttaagccaa accttcttca attcttcacc tctaaaatgc gtgttcgtcg tcatcgcaac
    17941 cccaagtgat aaacatgttg ttaacataac attaactcat cttaccagct tgataattag
    18001 acaacgaaaa ggttgcgaag cgcgtcacta tttattttta tagttgccgt atgaacgcag
    18061 aaacagcgga ccagccgctg tttctttttc tttaaaaaca acttgttact cggttacaat
    18121 tgtttcaact cttgcagggc caggattata ccaaccccat ccgccgtaac catagggcca
    18181 gccgcgtcca ccataaaacc atggatcaat tggctgcggc ggcataatca cctgttgagt
    18241 gatatgccag cgtttgtaac ctgttacctg cataaccata aatttatatg gtgcattatt
    18301 tactttgcca tccactgtgc cagtgattgg cccgacaacc gtcaccagtt gtccacgaaa
    18361 atcgaccgga tcaagaaaac cgttcacatc ggcataaatg cgcccgcgtg aaggttctcc
    18421 caaggttggt cttgctccgc tgtccagcgg tacggtagca atttccaggc gggttttccc
    18481 ttgctggttt tgtaccacaa ccactttgcc gccaaagcgc gcctcctgac caacatataa
    18541 atctggcgca ttcatcacgc gtaccagatc ttgctgtggt gttggactgc tgcctttaat
    18601 ggcgtccggc acagtgacac aaccgctcag cattagcgca aacgtacctg ccagtatgcc
    18661 tttgataaca ttcttttgaa ccgccatgat gcgactcctt tttttctcag ggcatactct
    18721 taagattcat tctttgcccg gaagtttctt ccatgcgacg ttgttacgta aataaaccgg
    18781 ttcggcatgt tccaccgcca ccgttttacc ctcggcaaac atctgacacg caatcggcag
    18841 catatcttcc gccgctggca gtaaaacttc tccgtcgcgc aaaaccagcc cgctctcttt
    18901 acggagatcc ggccaggctt gccagcccgt ccctaccgtt acccattcgc cggaaagctg
    18961 ttgcattcgt tcatgaacga gttcgggttt gagtacggct tcggtttctt caccgcgcca
    19021 gataccgttt tcatcacgct gatattcggc ccagtaaact tcgcccattc gcgcgtcaat
    19081 ggctgccagc acgcgggttg cgccgttttt gcgccacgcc ccctgcgcca tcgtcattag
    19141 cgtagagaca ccaatcatcg gtaactccgc gccaagcgcc agcccttgcg caataccaat
    19201 accaatgcgc acgccagtaa agctaccggg gccgcgcccg taagccagag cgtttatgtc
    19261 agtcagggaa gttccgctgg tggtcaggat atcctgcacc atcggtaaga ttcgttgagt
    19321 atgttcacga gggcaaagct caaaatgagc gttgacatta ccgtcgttcc acagagcgac
    19381 agagcagacc tctgtcgcgg tatcgatagc cagaattcgc atgggtcttc gtgcttagat
    19441 caataaaaag gcgcgcatca taccatactc cgtaacaaat tacctggagg atggtatcgc
    19501 aaggaaacga accgcacggg caatgtcacg ggtgcgtggc gcgggcggca gactggcgag
    19561 aaacgtcgcg ccgtaaggac gcatcaccag ccgattgtca caaattacca gcacgccgcg
    19621 atcgtcggcg tcgcgaatca gtcgccctac tccctgcttg agagtaatga ccgcatccgg
    19681 cagctgcact tcatcgaacg ggtcgccacc gcgcaaacga caatcttcca tgcgcgcttt
    19741 taacagtaga tcatccggcg aggtaaacgg caatttgtcg ataattacca atgacaatgt
    19801 atcgccacgc acgtccaccc cttcccagaa actactggtt gccacaagaa gcgcgttacc
    19861 ggcgctgaca aattgctgca acagttgccc tttgctggtt tccccctgca acaaaacggg
    19921 aagcgtcatg gtagcgcgga actgctcggc cagatcgcgc atcatggcat gcgaggtaca
    19981 aagcataaaa caacgaccgt tgttagcttc gatgatcggt cgcagcattg ccgccagttg
    20041 ccgtgcacaa cccggttgat tggtttgcgg caggttgcgc ggcacacaga gtaacgcctg
    20101 gcggctgtaa tcaaacgggc ttggcaatag caacgactcc gcctgttcta tcccaagccg
    20161 cgaggtgaaa tgatgcagat cgtcgttcac cgacagcgtt gctgaggtga agatccagct
    20221 accgggcttt tgcgccatta actctttgaa tttatccgcc acgctgagcg gcgtgagagc
    20281 cagagtaaaa tggcgcgaag tgcattcgta ccagtagctg tagcccggct gattgatctc
    20341 ttttagccgc ttcagccgtg tgcgatacaa cgtggcgcgc tcaaatgccg catccagcaa
    20401 ggcggaacgc cccagtgaca gtttcgccac gtcataacaa agttccaggg tgtcatcgag
    20461 cagtaaaaat gcccgctgaa tttgcggatt agctaacagc tcacgcaggt taccgcgata
    20521 acctggctca ccgagttgca gacgaaaatc ctgtgcactc tgggcaagac gatcagcgca
    20581 cttttgtaac tgctgggtgt cttttaattc ggtgcggtag gcgatggtga tgtcttttgc
    20641 caggtcgagc agttgtcgac tggagagtga ctgaccaaaa tactggctgg caatgtccgg
    20701 tagctggtgg gcttcgtcga agatcatgac gtccgcttca gggatcaact cgccaaatcc
    20761 actctcttta accaccatat ccgccagaaa gagatgatgg tttaccacca ccacatcggc
    20821 gtccatcgct tttttacgtg ctttgaccac aaagcaatct ttatacatcg ggcagtcgct
    20881 gccaagacag ttgtcgttgg tgctggtgac cagcggccac gcctgtgaat cttccgccac
    20941 gctgacgcag gtgctgatat caccatcgac tgtttgatta gaccaggagc gcagcaggat
    21001 cacatcgctt aagatttgta ccggcagatc gccccccgcc agcgcctgct gttcgagacg
    21061 ttcgaggcag aggtagtttg agcgcccttt cagcagcgcc acgttgcccg tatatttcaa
    21121 tgcctttgag actgttggca aatcgcggct gtagagctga tcctgcaacg cttttgagcc
    21181 ggtcgagata atgactttct ttttcgcccg cagcgcagga gccaggtaag cgtaggtttt
    21241 gcccgtaccg gttcctgctt ccaccaccag cggctggcct ttttctatcg cctgggtgac
    21301 ggctaccgcc atctgtcgct gtggttctcg cggcttaaag cctggtatcg ctttcgccag
    21361 ctgaccgtct ggtgcaaaat cgtccgtcac actaccccct gttgatttga acagggatta
    21421 tgtcaggatg agggcgcttt cgccagttga agtggtgacg gcaacctcac attgtggcag
    21481 tctttgcagc acgaaaatgg aagtataatg aggacaaaat gatgactatc gttcgtatcg
    21541 atgctgaagc ccgctggtct gatgtagtaa tccacaacaa cacgctctac tacactggtg
    21601 taccggaaaa cctcgacgcc gatgcctttg agcaaaccgc caacacgctg gcacagattg
    21661 acgccgtgct ggaaaaacag ggcagcaata aatcgagcat tctggatgcc accattttcc
    21721 tggccgataa aaacgacttc gcggcgatga ataaagcgtg ggatgcctgg gttgtcgcgg
    21781 gtcatgcgcc agtgcgctgc acggtacaag cgggtttgat gaacccgaag tataaagttg
    21841 aaattaagat tgtggctgcg gtgtaagctt tatcgaagca aaataagtca gacgataatt
    21901 tatcgataat actggtcggt tttacataaa tcgaccagag aataagatat tactgttgat
    21961 gcttctgcta attcattatt tatatttata atttcaattt tatctataaa actaactata
    22021 taaataaaca taaccatgcc gttgtgttcg ccctttgatt agcaactctg gttttctttc
    22081 ttagctgaac aatctggtta tagcgtgcgg actgaccgtt aattttctcc ttcttactgg
    22141 ataacacacc atgagctgct acctttaaaa cacctgaatg tcaacaggtt aactcgtgcc
    22201 atatcgttat aatccctttg atattagatg caa
//
LOCUS       DAJTZL010000056        22134 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.056, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000056 DAJTZL010000000
VERSION     DAJTZL010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22134
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.056"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..524)
                     /locus_tag="OO544_001866"
     CDS             complement(<1..524)
                     /locus_tag="OO544_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCR6767477.1"
                     /translation="MVAKINLLSRLTPLLFVFAPFLAQSNMTVYPMAVSINSQGEGNV
                     RVISKSNEVQYIKATVFRIDNPSTPQENEVEIKSGDANHLVVMPPKFALPAGSSKTVR
                     FVAMEPEQKEKNYRVKFEAVPSIDDVATDKKDLSMQLTVNLIWGIVVSVPPQQPIAKL
                     EVNAAQKLVNAGNQR"
     gene            820..1596
                     /gene="exuR"
                     /locus_tag="OO544_001867"
     CDS             820..1596
                     /gene="exuR"
                     /locus_tag="OO544_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HCR6767478.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            1941..2603
                     /locus_tag="OO544_001868"
     CDS             1941..2603
                     /locus_tag="OO544_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR6767479.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPIFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            2607..2990
                     /gene="mzrA"
                     /locus_tag="OO544_001869"
     CDS             2607..2990
                     /gene="mzrA"
                     /locus_tag="OO544_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HCR6767480.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            3137..3505
                     /locus_tag="OO544_001870"
     CDS             3137..3505
                     /locus_tag="OO544_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 domain-containing protein"
                     /protein_id="HCR6767481.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHHKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            3543..3848
                     /locus_tag="OO544_001871"
     CDS             3543..3848
                     /locus_tag="OO544_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HCR6767482.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            3851..4255
                     /locus_tag="OO544_001872"
     CDS             3851..4255
                     /locus_tag="OO544_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HCR6767483.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            4245..4544
                     /locus_tag="OO544_001873"
     CDS             4245..4544
                     /locus_tag="OO544_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HCR6767484.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            4730..5122
                     /locus_tag="OO544_001874"
     CDS             4730..5122
                     /locus_tag="OO544_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCR6767485.1"
                     /translation="MKKLEDVGVLVARILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSNFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            5192..6205
                     /gene="yqjG"
                     /locus_tag="OO544_001875"
     CDS             5192..6205
                     /gene="yqjG"
                     /locus_tag="OO544_001875"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005140183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HCR6767486.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYISLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESTEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPQDEMFASVKKRHLT
                     KCL"
     gene            6470..6835
                     /locus_tag="OO544_001876"
     CDS             6470..6835
                     /locus_tag="OO544_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCR6767487.1"
                     /translation="MDWYLKVIKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEP"
     gene            7077..7433
                     /locus_tag="OO544_001877"
     CDS             7077..7433
                     /locus_tag="OO544_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCR6767488.1"
                     /translation="MQWYLSVLKNYVGFSGRARRKEYWMFTLINAIVGAIINVIQLIL
                     GLELPYLSMLYLLATFLPVLALAIRRLHDTDRSGAWALLFFVPFIGWLVLLVFFCTEG
                     TSGSNRYGNDPKFGSN"
     gene            complement(7484..8380)
                     /gene="yhaJ"
                     /locus_tag="OO544_001878"
                     /pseudo
     CDS             complement(7484..8380)
                     /gene="yhaJ"
                     /locus_tag="OO544_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
     gene            8485..9186
                     /locus_tag="OO544_001879"
     CDS             8485..9186
                     /locus_tag="OO544_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HCR6767489.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMGKQQLIASPEGTMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            9209..9373
                     /locus_tag="OO544_001880"
     CDS             9209..9373
                     /locus_tag="OO544_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6767490.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(9507..10817)
                     /locus_tag="OO544_001881"
     CDS             complement(9507..10817)
                     /locus_tag="OO544_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HCR6767491.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVRIETHNG
                     VVFTQQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(10845..12176)
                     /locus_tag="OO544_001882"
     CDS             complement(10845..12176)
                     /locus_tag="OO544_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HCR6767492.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSPFLAFS"
     gene            complement(12451..13815)
                     /gene="tdcG"
                     /locus_tag="OO544_001883"
     CDS             complement(12451..13815)
                     /gene="tdcG"
                     /locus_tag="OO544_001883"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HCR6767493.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAAWMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(13887..14276)
                     /locus_tag="OO544_001884"
     CDS             complement(13887..14276)
                     /locus_tag="OO544_001884"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HCR6767494.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQACLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(14290..16584)
                     /gene="tdcE"
                     /locus_tag="OO544_001885"
     CDS             complement(14290..16584)
                     /gene="tdcE"
                     /locus_tag="OO544_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HCR6767495.1"
                     /translation="MKIDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(16618..17826)
                     /gene="tdcD"
                     /locus_tag="OO544_001886"
     CDS             complement(16618..17826)
                     /gene="tdcD"
                     /locus_tag="OO544_001886"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HCR6767496.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVVIDTEMNNRSNSFGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(17852..19183)
                     /gene="tdcC"
                     /locus_tag="OO544_001887"
     CDS             complement(17852..19183)
                     /gene="tdcC"
                     /locus_tag="OO544_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HCR6767497.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLVLKFGYKGDKTKVSLGKLNTISMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(19205..20194)
                     /gene="tdcB"
                     /locus_tag="OO544_001888"
     CDS             complement(19205..20194)
                     /gene="tdcB"
                     /locus_tag="OO544_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HCR6767498.1"
                     /translation="MHITYDLPIAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(20293..21231)
                     /gene="tdcA"
                     /locus_tag="OO544_001889"
     CDS             complement(20293..21231)
                     /gene="tdcA"
                     /locus_tag="OO544_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HCR6767499.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
     gene            21420..21764
                     /gene="tdcR"
                     /locus_tag="OO544_001890"
     CDS             21420..21764
                     /gene="tdcR"
                     /locus_tag="OO544_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HCR6767500.1"
                     /translation="MTGITIFYGDNIIRYVVNTKKGLRPYFKQLPDNYQAKFELNLMS
                     KFSNFIINKPFSAINTAARHIFSRYLLENKHLFYQYFKISNTGIDHLEQLINVNFFSS
                     DRTSFCECNRFP"
BASE COUNT         5803 a         5650 c         5168 g         5513 t
ORIGIN      
        1 cgttgattcc ctgcattaac taatttttgg gcagcattta cttctaactt agcaataggt
       61 tgctgaggtg gaacactaac aacaatcccc caaattaagt taactgttaa ctgcatagag
      121 agatcttttt tatctgtggc aacgtcatca atactgggaa ccgcttcaaa tttaacgcga
      181 taattttttt ctttttgctc tggttccatt gcaacaaaac gcacagtttt gctactaccg
      241 gctggtaaag cgaatttagg tggcataaca accagatgat ttgcatcacc ggatttaatc
      301 tcaacttcat tttcctgagg cgtcgatgga ttatcgatac ggaatactgt cgccttgatg
      361 tactgaactt cattactttt agatattacg cgaacgttgc cttcaccctg actattaatg
      421 gaaacagcca taggataaac agtcatattt gactgtgcca aaaaaggagc gaacacgaat
      481 aaaagcggcg taagccgtga taagagatta attttcgcca ccatataaat ttgatcttac
      541 aatattgttt ccaaaaagtt ggtgtattag agatgtagat ttattgtaag tcaatataaa
      601 aaagcatttt tagttaatat taaatttaaa ggctgggaat taacttactg aaatttagta
      661 gctttcacaa tgacgatact tattttttag gaatttataa atattcacaa tattaattca
      721 atacattaat tcaatcacca aatttatata tgaccaatgg catacccatg aataaagtgg
      781 tatgataact tcgtccgcat tccactccgg gagacgcata tggaaatcac tgaaccacgc
      841 cgtttgtatc aacaacttgc cgctgacctg aaagagcgca ttgaacaggg cgtctatctg
      901 gtgggtgata aactgcctgc cgaacgcttt attgccgatg aaaagaacgt cagccgaacg
      961 gtagttcgtg aagccatcat catgctggaa gttgaaggct atgtggaggt gcgtaaaggt
     1021 tcaggtattc atgtggtttc caaccagccg cgccatcagc aggcggctga caataatatg
     1081 gaattcgcca attacggtcc gtttgagttg cttcaggctc gccagctcat cgaaagtaat
     1141 attgccgagt tcgcggcaac tcaggtaacg aaacaagaca tcatgaaact gatggccatt
     1201 caggaacagg cgcgcggcga acaatgcttc cgtgattccg agtgggattt acagttccac
     1261 attcaggtag ccctggcgac gcagaactcc gccctggcgg ccatcgttga aaaaatgtgg
     1321 acccagcgta gccataaccc gtactggaaa aaactacacg aacacattga ttcccgtacc
     1381 gttgataact ggtgtgatga ccacgatcaa attctcaagg cgttaattcg caaagatccc
     1441 catgctgcta agctggcaat gtggcagcat ctggaaaaca ccaaaatcat gttatttaac
     1501 gaaaccagcg acgatttcga gttcaatgcc gaccgctatc tattcgccga aaacccggta
     1561 gtgcatctcg atacggccac cagtggcagt aaatgaaact cctgtccgac aggcgctgcg
     1621 acgcgcctgt tcgcttcgtc ggtaagcgaa aagtataaag tgtcagcctg tgtaaatcct
     1681 ctcgccaccc tcccctgcat tcagcaaaat cagactccac ggacatgtaa ttttgataac
     1741 gaacaacgtt gacctttgtt acaattagat tcaatttgaa tttatgtttt tgaatgcttt
     1801 cttatctcac gatttaacag ggaatagttc aggctgtgtt gatgtatcaa acccgcagaa
     1861 cataccaaaa cagcaataac attgcggtag tgcatctttt aaaaccagcg tggcgttaac
     1921 cgattcacca ggaataatga atggaacttt tgacccaatt gctgcaagcc ctgtgggcgc
     1981 aggattttga aaccctggcc aatccatcga tgattggcat gttgtatttt gtcttgtttg
     2041 taattttgtt ccttgaaaac ggcttgcttc cagcggcctt tttaccgggc gacagtttac
     2101 tggtattggt cggcgtgttg attgcgaaag gcgcgatggg ttacccgcaa acgattctgc
     2161 tgctgaccgt tgccgccagc ctcggctgct gggtcagcta tattcagggg cgatggctgg
     2221 gcaatacccg caccgtacaa aactggctat ctcatttacc cgcgcattat catcaacgcg
     2281 cacaccatct ttttcataaa cacggtttat cggcgctgtt aattggtcgc tttattgcgt
     2341 ttgtcagaac actgctgccg acgattgccg ggttatcagg gctgaataac gcgcgctttc
     2401 agtttttcaa ctggatgagc ggtctgctgt gggtattgat cctgacaact ctgggttaca
     2461 tgctcggcaa aacgccgata tttttaaagt acgaggacca gctgatgtca tgcctgatgc
     2521 tgctcccggt ggtgctgctg gtgtttggcc tggcaggttc tctggtcgtg ttatggaaaa
     2581 agaaatatgg aaatcggggg taagggatgc aaatacctcg catgtcgctt cgccagctag
     2641 cctggtccgg cactgtttta cttctggtcg gcacgctgtt gctggcctgg tccgcggttc
     2701 gccagcaaga gtctacgctg gcgattcgtg ccgttcatca aggcacaacg atgccagacg
     2761 gtttttcgat ctggcatcac cttgacgctc atggcattcc tttcaaaagt atcaccccca
     2821 aaaacgacac tctgttaatt acatttgact ccagcgacca gagcgccgcc gcaaaagcgg
     2881 tcctcgacag aacattgccc catggctaca tcattgcgca gcaggacaat aacagtcagg
     2941 ctatgcagtg gctgacccgg ttacgggata actctcatcg cttcggataa cttcctgtta
     3001 tccgaaacat atcactcact ttggtgattt caccgtaact gtctatgatt aatgaggcgg
     3061 tggttaacac taccgcttcg ttgtctggac aatcgttcct ttgtaatagg tccgaatcac
     3121 aatggaaggt tcaagaatga aataccgcat cgctttagct gtttctctct ttgctcttag
     3181 tgccggtagt tatgccacta ccctgtgtca ggaaaaggag caaaatatcc ttaaggagat
     3241 cagctatgcc gaaaaacacc aaaaccagaa tcgtattgac ggtctgaata aagccctgag
     3301 tgaagtccgg gccaactgtt cagatagcca gctgcgtgcc gatcatcaca agaaaatcgc
     3361 aaagcagaaa gatgaggtgg cggaacgcca gcaagattta gccgaggcga agcaaaaagg
     3421 cgatgccgat aagattgcca aacgcgaacg gaaactggca gaagcgcagg aagagctgaa
     3481 aaagctggaa gcgcgcgact actaactcac aatagtcact acttactcac ctggagaaaa
     3541 ctatgtcgaa agaacacact acggaacatc tgcgtgctga gttgaaatcc ctttccgata
     3601 cgctggaaga ggtgcttagc tcatctggcg agaagtcgaa agaagagttg agtaagattc
     3661 gtagcaaagc ggagcaggca ctgaaacaga gccgttatcg cctgggtgaa accggtgatg
     3721 ccatcgccaa acaaacccgt gtcgcggcgg cgcgtgctga tgagtatgtg cgtgaaaatc
     3781 cgtggaccgg cgtgggaatt ggcgctgcaa tcggtgtagt gctcggcgtt ctgctgtcgc
     3841 gtcgttaatt atggcggaca ctcatcacgc gcaagggccc ggtaaaagcg ttctgggcat
     3901 cgggcagcga attgtttcca tcatggttga aatggtggaa acacgtctgc ggctggcggt
     3961 ggtggagctg gaagaggaaa aagctaatct ctttcaactt ttactgatgc tgggcctgac
     4021 gatgcttttc gctgcatttg gtcttatgag cctgatggtg ctaattattt gggcggttga
     4081 tccgcaatat cgcctgaatg cgatgattgc caccaccgtg gtgttgctgc tactggcact
     4141 gattggcggt atctggacgt tacgtaaatc gcgtaaatcc accttgctgc gccatacacg
     4201 ccatgagctg gcaaacgatc ggcaactgct cgaggaggag tcccgtgagc agtaaagtcg
     4261 aacgtgaacg acgtaaggcg caactgctta gccagatcca gcaacaacgg ctggatcttt
     4321 ccgccagtcg tcgtgaatgg ctggaggcaa caggcgctta cgatcgtcgc tggaatatgc
     4381 tgctaagtct gcgctcctgg gcgctggttg gcagtagcgt gatggcgatc tggacgattc
     4441 gccatcctaa tatgctggtc cgctgggcca gacgcggttt tggcgtatgg agcgcctggc
     4501 gtctggttaa aacgaccctc aagcagcaac agcttcgcgg ttaacttccc tctggccgga
     4561 gcaattccgg ccttatccct caaatttttt gaagattttt gacagttttc cttgctaaca
     4621 atcatcattc accacgttta tgattctctc catcgacagc aacgacgcta ataccgcgcc
     4681 cttgcacaaa aaaacaatca gcagcctgag tggcgcagtg gagagtatga tgaaaaaatt
     4741 agaagatgtt ggtgtactgg tagcgcgcat tttaatgccg attctgttta ttaccgcagg
     4801 ctggggaaaa atcaccgctt actctggtac ccaacaatat atggaagcaa tgggtgtccc
     4861 gggttttatg ctgccgctgg tgattctgct tgagttcggt ggtggtctgg caatcctgtt
     4921 cggtttcctg actcgcacca cagccctgtt tactgcgggc tttacgctgc tgacggcatt
     4981 tttatttcac agcaactttg ctgaaggcgt caactcgctg atgttcatga aaaacctgac
     5041 aatttctggc ggattcctgc tgctggcaat taccggtccg ggcgcgtata gcatcgaccg
     5101 cctgctgaat aaaaagtggt aagcacgcgc tatacttaac gataaaaaac gaggaggaag
     5161 ctcctcgttt ttgctattgg aggagagaaa aatgggtcaa ctgattgacg gcgtctggca
     5221 tgacacctgg tacgatacca aatctaccgg cggtaaattt caacgttcag cttccgcatt
     5281 tcgtaactgg ctcactgccg atggcgcacc tggccccact ggcacaggcg gttttatcgc
     5341 agagaaagat cgttatcatc tctatatttc actcgcctgc ccgtgggcgc accgcacgct
     5401 gatcatgcgc aaactcaaag gactggaacc gtttatttcc gtttccgtag tgaacccgct
     5461 gatgctggaa aacggctgga cctttgatga cagttttccg ggagcaaccg gagatacgct
     5521 ctatcaacat gaatttctgt atcagcttta tctccacgcc gatccacact acagcggacg
     5581 agttactgtt cccgtactgt gggacaaaaa gaaccacacc atcgtcagca acgaatcaac
     5641 agaaatcatc cgcatgttca ataccgcgtt tgatgcgctg ggcgcgaaag cgggtgatta
     5701 ctacccacca gccctgcaaa cgaaaattga cgaacttaac ggctggattt atgacaccgt
     5761 taacaacggc gtgtataaag ctggttttgc caccagccag caagcctacg acgaggcggt
     5821 ggcgaaagtg tttgaatcgc tggcgcgact ggagcagatt ttaggccagc atcgttacct
     5881 gaccggcaac cagctaaccg aagccgatat tcgcctgtgg actacgctgg tgcgttttga
     5941 tccagtgtat gtgacccact tcaagtgtga taagcaccgc atcagcgatt acctgaatct
     6001 gtatggcttc ctgcgcgata tctaccagat gccggggatc gccgaaactg tcaatttcga
     6061 tcacatccgt aatcattact tccgcagcca taagaccatc aaccctacgg ggattatttc
     6121 aattggtccg tggcaggatc tcgatgaacc gcaggacgag atgttcgctt cggttaaaaa
     6181 aaggcacctt acgaagtgcc tctgataatt agctaagtgc atgttgttca tatcggatgc
     6241 ggcgtgaacg ccttatcaaa cctacaaaac atttatattc aatatgttgc aatgaccgcg
     6301 caggccagat aagcgtagcg catctggcga ttttgcgctt gtcatcagtc ttagcgcgcc
     6361 ttcacagcac tttattaatt ccttgaacga atatttactg ccatttcatt cacgtctatt
     6421 cttaatttgc tgctttaaaa acaagtgatt gagcaacatt gaggcaaaaa tggactggta
     6481 tctgaaagta ataaaaaatt atgtcggttt ccggggtcgt gcgcggcgca aagagtactg
     6541 gatgtttatt ctggtcaaca tcatctttac gttcgtgctg gggctgctgg ataaaatgtt
     6601 aggctggcaa cgcgctggtg gcgaaggcat cctgacgaca atctacggta ttctggtgtt
     6661 tttaccgtgg tgggcagttc agttccgccg cctgcacgac accgaccgct cggcgtggtg
     6721 ggcactgcta ttcttaatcc cgtttatcgg ctggcttatt atcatcgtct ttaactgcca
     6781 ggcgggtacg ccaggcgaaa accgctttgg acctgatcca aaactggaac cttaaaacaa
     6841 atctgctcgc cggatgtctg actcatccgg cttttatcat catgttgatg aatgaataat
     6901 ttactaatgt ttatttaaaa tatttcaatg tcgttatata aattacctat aaaaaataac
     6961 catgtattta tcttaaatat caaaaccttt ataacatccg aaaaagaaag atacgaatag
     7021 catttacaca atacccggaa tagactttcg tcacttttaa ttaaagggat gtttttatgc
     7081 agtggtattt atccgtatta aaaaattatg ttggtttctc tggtcgcgca cgtcgtaaag
     7141 agtactggat gtttactctg attaacgcca tcgtcggcgc tattatcaat gtcattcaat
     7201 tgattttagg tctggagctt ccatatctgt ctatgcttta tttgctggca accttccttc
     7261 ccgtccttgc gcttgctatc cgtcgcttac acgataccga ccgttcaggt gcctgggcct
     7321 tgctcttttt tgttccgttt attggctggc tcgtgcttct ggtttttttc tgcacagaag
     7381 gtacttctgg cagcaatcgt tacggaaacg atccgaagtt tggttcaaat taatcttaga
     7441 attggggcga tatttcgccc ctttttatta acaataataa atattatttt ccgctaaaaa
     7501 gtttgggaat ttcccgcaga caccaggatt tcgcttcccc catactgtca cgacgccagg
     7561 ccataataat atcgatctca ctggtcgatt ccgggctgac gacacgcaac cgcccttccg
     7621 caatatcttt ttcgaccatc ggatacggca tcgtcgccac gccaagcccc gccagtaatg
     7681 cctgacgttt atcttcaatc gtgctcaccg ttaagcgcgg ctgtttgtcc agcagttgta
     7741 cggtcaacac cgggcgctca cgagcggtat ccgctaccgc aattccacga tatttcacgc
     7801 gcgtcacttc agataacggt tccggctcct gatgaatcgg gtgatcaggc gcggcaacgt
     7861 agacgttcat taacgtatag agcttgcgcg agttgatctc cgacgaggaa cgaaaatgca
     7921 tatccggcgc gataacaata tccgcccgcc cctgctccag tcgttcctac gcccccgcca
     7981 gcacttcggt gatgattgcc agttgggtat tggcttttgc cgccagtttg tcgattaacg
     8041 ggaaaaaggc aggtgtcggt accagcgctt cggtcacaat ggtgagatgc gtttcccaac
     8101 cgcgcgcgag ggcttccgca tcggtagtca gtttatctgc ggcttccagc aaaacgcgcc
     8161 ctcgctccag caacatcctc ccgacattgg tgaatttggt gcgatggccc gagcggtcaa
     8221 acagcaccac atccagctct tcttccagtt tttgcatggt gtagctaagt gcggaaggca
     8281 cgcgtcccag ctcatccgcc gccgccgcaa aactgccccg gcgatcgatc gcatccataa
     8341 cccgtagtgc ttccagcgtt aatgcccttt ctttggccat ttcgttctca ttcaggaaat
     8401 ttgaacatac cgggcagaat atctggctaa caatgtagcg tccagcccct taccataaaa
     8461 ggaagtaaag agaggtcaag aattatgatt actacccgaa ctgccaggca gtgtggacaa
     8521 gcagactacg gatggttgca ggcccggtat actttttcct ttggacacta cttcgacccg
     8581 aaattgttag gctatgcctc cctgcgtgtg cttaaccagg aagtgctggc cccaggtgcc
     8641 gcctttcagc cgcgaaccta tcccaaagtc gatattttaa atgtgattct ggatggagaa
     8701 gcagagtatc gcgacagcga aggcaatcat gttcaggcca gcgccggtga ggcgttgctg
     8761 ctctctaccc agccgggtgt cagctacagc gaacacaatc tcagcaaaga caaaccgtta
     8821 acacgaatgc agctttggct ggacgcctgc ccgcagcgag agaatccgct gattcaaaaa
     8881 ctggcgctta atatgggcaa gcagcaatta atcgcctcgc cagagggtac gatgggaagc
     8941 ctgcaattac gccagcaagt gtggctgcac catatcgtgc tcgacaaagg cgaaagtgcg
     9001 aatttccagt tgcatgggcc acgcgcgtat ttgcaatcga ttcacgggaa atttcatgcg
     9061 cttacgcatc atgaagagaa agcggcgctg acctgcggtg atggggcgtt tattcgtgac
     9121 gaggctaaca ttacgctggt tgccgattcc ccactgcgcg ctttgctgat agatttgcct
     9181 gtctagttgt ttttacaggg agatgatgat gagtaaaaaa tcggccaaaa agcgccagcc
     9241 agtgaagccc gtggtggcga aagaacctgc tcgcaccgcc aaaaattttg gctatgaaga
     9301 gatgttgagc gagctggaag ccatcgtcgc ggatgctgaa acgcgtttag ccgaggatga
     9361 agctaccgcg taaagacagg ttgccggatg cggcgaaaac gccttatccg gcctacaaaa
     9421 tccaacaaca ttaacgaatt aaacaacccg taggccggat aaggtgcgcc agcatcgcat
     9481 ccggcacaat actcaaaacc tggcgtttat ctggccttgc tcgccataat ctcgataatc
     9541 tgccgatccg tttgctgcat cgaatggctt gctaacgcac acaggttggc aatcgactgc
     9601 tcaacatcat gcgccacgat cccttcattg ccggtcacgg cggtatcatc cagcgccatt
     9661 aacaccgctt tccacgcagc cgaagcactg gtcgaaacct tcatcgcgca gctgttcgac
     9721 gcaccatcgc aaatcatgcc gctgacatcg ccgatcatac tgctgatcgc catcgagata
     9781 gtttcataac gcccatccac cagccatgcc atcccggcgg cggcccccat tgctgcggtc
     9841 gttgcggcac aaagtgcaga caaacgcggt aactggttat ggatgtaaat tgcgctcaaa
     9901 tgagaaagca tcagcgcacg cgccagtcgt tcatcatccg ctccgaagtg ttctgctaca
     9961 accaccacag gcattgttgc ggtgatcccc tggttacccg agccggagtt actcatagcc
    10021 ggaagcgtag cgccgcccat acgcgcatcg gatgccgcgc tggtacgaat cacaatggat
    10081 gaagagagat ctttcgccag caaaccgcgc tcgcactgtt tttccagcgt cgcgccaata
    10141 tgcagccccc acttaccgct caaaccttcc tgcgataacg cacaatttag cttcgcggaa
    10201 tcgagaataa agcggatcgc cgcaaacggg acttcattga cgaacttcag gatctcagcc
    10261 agcgtcgttc tggaaagcac cgtcagcgga gactcttgct cgccctctgc cacacacgcc
    10321 tgctgggtaa acaccacacc attgtgcgtc tcaatacgca caatgttggt atgcccgccg
    10381 acgatggtga cacacgccca cttctcaccg ttccagactt tggcgcgtga gaagaggatt
    10441 tcattgcaag gttcctggat cttaacggag actttccccg ccgccagcag tgctttggca
    10501 tcggcaattg cctgcgcagt tgcgtctttc agcacttcca gcccggcgtt ggcatttcca
    10561 cctaacgccc ccagcgccgc cgcaatcggc agccccacca ttcccgtgcc gggaacggtg
    10621 acgcccagac cgttcttcat cagatttggc gaaacccagg cttctacacg ttcaaccgga
    10681 ccttccagtt ctgccgcagc aaccgccgcc gccagcgcca gtgaaatcgg ttcagtacat
    10741 cccagcgccg gttttacttc ctcctgaacg gcgaggatgt aacgctgcca taacggattt
    10801 aaagtcgaat caaacatatt aaaaacctta aaatttcagg taaatcaaga aaatgccagg
    10861 aacggagaaa cacaaagcaa caaaccagtg acgataatca ggtacagaga catccctttg
    10921 tatttgtgca atgccggtac tttgtaaacc agccacgccg ggatcaggca ccctaccatg
    10981 ccgaaaatcg ggctacagat agaggtgaag ctcaacaccg gtgcgttcag tacgatagcg
    11041 ctccaggcca gcaaaatggc gaaaatcatg atgccgcgct gaacaagatt ttcgttaatc
    11101 ttctcggcag gcatcttgcg acgcaggatg ttcattacga tcccttgcgt tgcttcgcga
    11161 aagcctaaat agacgccaaa gaatgcagtc atgacagcaa agatattgag aatgacgctg
    11221 accactttca cccacgctgc gccgtcaccg ctaataaact gtgcggcaat cgccagcgcg
    11281 gaaatattct gctcgtaggc ttttaccgct tcgtcatgcc ccatcgccag cgtgaacgac
    11341 acggcgtaga aaaagacggt gacaaacaaa atgccaaacg cgatattcat tgcccgcaat
    11401 gctttatgcc gcgccacttc aatagatttt tcccgcgagc gataagagat caccatcggg
    11461 cttaacgtct ggataaacaa aatcgacgtc agggtaaacg gcagcgtaat aatggcgttt
    11521 ttcaccagca gccccagcgg cggtagcgaa ccgacgttat acagatgcca cattcccacc
    11581 atcgacacgc ccagcgccgc gaccaccagc agcttggtca gcaccatgcc ggtcgaaatt
    11641 ttgaataaca atttctcgcc gcgtgaagag atcgccacca gaatgcaaat cagtaccaga
    11701 ccataaaagg gactgtctga cagcaaccct tccgtcacgc cgaaggtatg cagataggaa
    11761 gcactatcgt tggtgatggc ggtggaatag acgaacatcc aaatcaccag cattacgaaa
    11821 tagagcgcac ctaacaggat gccccagttt ttacctaaat aaccgctaat gacactcggg
    11881 taatctttac attctggtga ttctgccagc gtattaataa acaaccgctg aaacagatac
    11941 attgccgggt aaccaatcac cgatgagagc aaaaataccc acaatcccat caaaccgacc
    12001 tgcaccggga gaaaaacaat ccccgcgcca atcgccatcc cgatactcat aatcacccag
    12061 ccggtgtcag tactgtcgaa tttgatcgct tctcgccact cgctctcact cattccagca
    12121 cgacccgccg gggtcgaagc gtctgcaatg acgcctttat tcgatgcgat ttccataatt
    12181 tctcgctcaa tattttgtag ggcttattat ttttttccga gccgcatcaa ggcgatatgc
    12241 ggtctacgtt tttgcaggcg agttattaga ataaagaaat gatacgcgcg aggtcagaga
    12301 aaatcttcac aatcaagact ttgaaataac ggagatggat aagaattttc tactaaatta
    12361 atcgcaagag aatattcatt ctctatttgc ggcagatcac aaaaaaaagg tgcacatttg
    12421 tgcacccaag gatgaaagct gacagcaatg tcagccgcag accactttaa tggccagtcc
    12481 tccgcgtgat gtttcgcggt atttatcgtt catatctttg ccggtttcat acatcgtctc
    12541 gatcacttta tcgagtgaaa cacgcggtgc cgaggtgcgg cgcatcgcca tccacgcggc
    12601 gtttactgct ttcacggcat taatggcatt acgttcaatg cacgggattt gtacctgtcc
    12661 ggcaaccgga tcgcaggtca gcccaaggtt atgctccatc gcgatttccg ccgcattgca
    12721 tacctgcgcc ggactgccgc ccagtagttc agttaaccct gccgccgcca ttgaacaggc
    12781 cacgccaatc tccccctgac agccgacctc cgcgccagag atggaggcgt tcattttata
    12841 cagcgcgcca atagcccccg ctgccagaaa atagcgggca attgaccgct cgtttaccgg
    12901 acgacggaac ttatcgtaat aagccagcac tgccggaata atgccgcacg caccgttagt
    12961 cggtgccgtt accacgcgcc cgccagctgc gttttcttca ctaaccgcca gcgcgtacat
    13021 gttgatccag tcgatgacat tcatcggatc gttagagatg ttatcgctag aaaccagctg
    13081 acgacgcagc gctacggcac gacgcggcac attgagcgga ccaggcagca cgccttcagt
    13141 gttcatcccg cgttcaatac cgtcatgcat cacttgccag atacgggcaa atcctgcgtc
    13201 aatttccgct ttgctgcgca gcgccagctc gttgtgcatc atcaggccag atatagacag
    13261 gccgttgtaa tcacacattt tcagcagttc acctgctgag tggaaatcgt aaggtacgga
    13321 cgtttcgaca tcgtgcgaca ggccgaagtg ttcttcttcg acaataaacc cgccgccgac
    13381 agagtaatag gttttactta atagcgcttc ctgtcccttc caggcagtga tccgcattcc
    13441 gttctcatgg cgaggcaaca tttcgggatg gaagataatg ttctttgcta caggaaaatc
    13501 aacaatatgc gcaccagatg ccactggcag ccgcccgctg cgcgttacta actctataaa
    13561 tgcagggatc tcatcaatga caacatcctg cggactgttt cctgccagtc ccatgataat
    13621 ggcgacatcc gtggcatggc ctttgcccgt cagtgacaac gacccataca gatcgaccac
    13681 aatatggctc gtcgcggtta ataagccgct actttccagc cgatcaataa aactttttcc
    13741 ggcattcatt ggccccacgg tatgcgaact ggagggacca atcccaattt tgaaaatatc
    13801 gaatgcacta atcatatcca caccctcgga ttgccgttca gtgaagtgga gcggaacgac
    13861 cttgcgaccg tcccgctcac gaggctttac gcactacgta ctgcgatagc ttcaatttcc
    13921 agcttcacat ctttcggcaa acgcgcaacc tgcacacagc tccgggtcgg gtaggtcgcc
    13981 tgatgctcat cgaagaactg cttatagact tcattgatgg tggcaaaatc attcagatcg
    14041 gtgatgaaca cggtcatctt gatgatatcg cccacgctca gcccggcagc aaccacgatc
    14101 gctttgacgt tttcgaggct taaacacgcc tgatcttgca catcagccgg gatctcaccg
    14161 gtctgcgggc aaaccggaat ttgcccggag gtgaagacca tgctgcctaa atcaacgccc
    14221 tgaacataag ggccgattgc gcctggggca cgttgcgttt cgataatctt tttcatacat
    14281 cctccggcgt cagagcgcct gggtaaaagt acgtgaaata acatcctgtt gctgttcacg
    14341 ggtcagtgcg ttgaagcgca cggcgtagcc agagacacgg attgtcaggt taggatattt
    14401 ttccgggtgc tcgatggcat ccagcagcat ttcccgattc attacgttga cgttgaggtg
    14461 ttgaccgcct tcgacatccg cttcgtggtg gaaataccca tccagcaggc cgacaaggtt
    14521 ggttttacgt actggatctt ctttgcccag cgccgcagga acgattgaga aggtgtacga
    14581 gatcccatct ttggcgtagg tgaacggcag tttcgccacc gacgtcaatg aggccacggc
    14641 acctttgcgg tcacgaccat gcatcgggtt agcgcccggc gcgaacggtg ttccggcgcg
    14701 acgaccgtcc ggcgtattac cggttttctg gccgtacacc acgttagaag tgatagtcag
    14761 aatcgactgg gtagggacgg cgttgcgata ggttggcagc gctttaattt tcttcataaa
    14821 gcgttcaacc aggtcgcagg caatgctgtc tacgcgctcg tcgttgttgc cgtactgcgg
    14881 atattcaccg tcgatttcaa agtccaccgc caggccgttt tcgtcacgga ttggtttcac
    14941 gcgggcatat ttgatggcag acagggagtc cgtcgccacc gacaggcccg cgatgccgca
    15001 tgccatagtg cgatagacat cacgatcgtg cagcgccatc agcgaagctt cgtagctgta
    15061 cttgtcgtgc atgtagtgaa tgatattcag cgcgctgatg tactgcaccg ccagccagtc
    15121 catgaagtga tcgaggctgt ccatcacttt gtcgtaatcc agcacgtcgt ccatcagcgg
    15181 tgctgttttc ggcccgacct gaatcttcag cttctcgtcc accccgccgt taattgcgta
    15241 cagcagtgtt ttcgccaggt tagcgcgtgc accaaagaac tgcatttgct taccaatcac
    15301 cattgggctg acgcaacaag caattgcgta atcgtcgctg ttgaagtcag tacgcatcag
    15361 atcgtcgttt tcatactgca aggaagaggt gacgatcgac acctgcgcgg catatttttt
    15421 gaaggcaatc ggtaattctt ccgaccaaag aatggtcagg ttaggttccg gtgccggccc
    15481 catagtgtgc agggtgtgca aatagcggaa ggagtttttg gtcaccagcg tacgaccgtc
    15541 cagccccatc ccgccgatca cttccgtcgc ccagattggg tcgccggaga acagcgaatc
    15601 aaattccggc gtacgcaaga agcgcaccat acggatcttc ataatgaagt gatcgatcag
    15661 ttcctgtgcc tgctgctcat tgagtacgcc tgctttaaag tcgcgctcaa tgtagatatc
    15721 gaggaacgac gccgtgcgcc ccagcgacat cgcgccgccg ttctgtgatt tcactgccgc
    15781 cagataagcg aagtagagcc actgcaccgc ttcctgcgca ttctgcgccg ggcgagagat
    15841 atcaaagcca tatttcgccg ccatttcctg aatctgcaac agcgcatgac gatgctctgc
    15901 cagctcctca cgcagacgga tggtggcttc cagatcttcg cctttttcca gacgggactg
    15961 gagatcggca aattgcagtt cgcgttcacg caccagataa ctgatgccat acagcgctac
    16021 gcggcgatag tcaccgataa tgcgcccacg gccatagcca tctggtaaac cggtcagcac
    16081 gccagattta cggcagcgca gcatatccgg tgagtaaaca tcaaatacgc cctggttatg
    16141 ggttttacgc agatcggtaa acagatattc aaattcactg tccatttcac ggccataggc
    16201 gtggaatgaa cttttaatca tattaatgcc accgaacgga tgcagcgcgc gctttaatgg
    16261 cgcatccgtt tgcagaccaa caattttttc cagcggctgg ttaatatatc ccgcatcatg
    16321 agcggtaatt gtggtggcaa tattggtatc gaaatcaacc ggcgcgtggg ttgcgttttc
    16381 gatacggatg ccttccatta ctttttccca caattccgtg gtggcaggcg tcgcttcggc
    16441 gaggaaagat tcatcgcctt catacggtgt atagttatgt tgaataaaat cgcggacatt
    16501 aatttcgttt ttccagtccg tacctttaaa gccaagccat gcgtcggcgt acagcttatc
    16561 gctggtatca atatctatct tcatgaaaaa taatctctct acaatacttc aactaaatta
    16621 tgcaaattct gcgggcgcgt taactttgcc taaatgaatg gcatccaaag caatcatttt
    16681 ttcttcgtta gtcggaataa cggcacaaat gacacgcgca ttttcactgg aaacaattcg
    16741 ctctccaaaa gagttagagc gattattcat ttctgtatca atcactacgc ctaatacagc
    16801 caaatgttcc atgaccagac gacgaattaa acttgaattc tctcctattc cgccggtgaa
    16861 tataattcca tccaggcgac gtaatgaagc tgcgtgtccg gcaatatgac gggcaattcg
    16921 gtgaacaaag gttttaattg ccagttgcgc gcgttcgtga ccttcatgcc aggctttttc
    16981 cagaacacgt aaatccgaag aaagaccgga aatacctaat aatcccgact ctttattcac
    17041 tacgcgttcc aggtcaccca ggctctggtt ggtttggctg gcgacccagg acatcgcacc
    17101 aaagtcgaca tcgccactgc gggttcccat catcaagcct tccagcggcg tcattcccat
    17161 tgaagtatca acactctgac cgttgcgaac cgcgcagatt gacgcgccat tgccaagatg
    17221 cgccacaacc aggccggagt catcttccgc cagattcagc agagaatgtg cgcgctggga
    17281 aacatagcgg tgcgacgtgc cgtggaaacc ataacggcgt acacctaact cttcataata
    17341 tttccacggc aggccgtata aataagcttc cggagccatc gtctggtgga aactggtatc
    17401 aaataccgcc acctgagtta cgcccggaaa taattgctgc gccgattcaa taccacttaa
    17461 attggcgtaa ttatgcaggg gtgccagtgg agaaacgcga cggatattat caatgacttc
    17521 atcggtaata atggcggact cggtaaaaat actgccgccg tgagcgatgc ggtggccaat
    17581 taaggccaca ctgtcattta aattccgttt ttccagttca aatgcaattg ccttcaatgc
    17641 accttcgtag ctgtggtgag ccagcggtgc tggctctccc ccatttacgg ataagaatgc
    17701 attttccgag ttaataccgt cggcaatacc tgacattaat acttcacagt cgctggcatc
    17761 gagcacggaa aacttaatcg aagacgaacc acagttaata accaaaacaa ccggaaattc
    17821 attcatctct tttctcatcc tgagttacgg attaaaacag tttgtatacg atgttcagga
    17881 tggtcagcag accaatcacg gtaacaaaca cgttatccag acgaccacgg tatttcgcca
    17941 gagacggcgc tttacggatg gcatacatcg gcaacaggca cagcagggat gcgataatcg
    18001 gtgcgcccat ggcttcaatc aggtcgagga tgttcgggtt ggcgtaggca acaacccagg
    18061 tggagcccat gatgaagatc atgctgatag tgttcagttt acccagcgac actttagttt
    18121 tgtcgccttt ataaccaaac ttcaggacca ggccattcag accttccagc gttcccagat
    18181 agtgaccgaa gaaagatttg aagatagcca cgagtgcgat gatggaagcc gcatattcca
    18241 gtgtaatcgc gaacgttgtt ttggtaccgg tcatggacgc aaagtggtta gccagataag
    18301 aaagcactgg aatattctgc gctttggctt ccgccatgtt ggccggagac agcgtaaata
    18361 ggcagctaaa ggcaaagaac atcaccactg caaccatcag catgctggca cgagaaatga
    18421 tttgggaaca tttacgttcg gtgaagtcgc gaccgaagtc tttctcatac tcttcacgtt
    18481 tagaaaccac gaaggaagag acgattggcg agaaattaaa ggagaaaacc atgatggaaa
    18541 tccccagcca gacggtgatc aggataccgt catgaccggt taacgacagc gaaccgaggt
    18601 caacctggtc gataactgca gagttccagt aagggatcag cgacagagaa atcagcacca
    18661 ggctggcgat aaacggccat accaggtagc tcatcacttt aaccatcaga tccttaccaa
    18721 accagatgac gaaagccatc agcagcagca ggaacagcgc cacaaagccg cgattcagcg
    18781 gtgcaaagcc gagctggttt tcccagaacg tcataaaggt attggtaata gtaacgccat
    18841 aaatccacag cagtgggcaa atcgcgaaga agtacaggaa cgtgataacc acgccgccag
    18901 ttttaccaaa atgctcttcc accgtttccg taatgttgcc ggaagggtta gagccagaaa
    18961 gacatagacg cgccagcgcc cggtggcaat aaaatgcgat ggggtatgcc aataccaaca
    19021 tcagaagaat cgggatcagt ccgccaaaac ctgcgcggat agggaagaac agcaccccgg
    19081 cgccgattgc cgtaccaaac aagcctaacg tccatgtggt atctgattta cgccaggacg
    19141 attgttttgt ctggctggat acaatgctat ctgaagtact catatcctat cctcaacgaa
    19201 ttaattaagc gtcaacgaaa ccggtgattt gagagacgcg agaaagatcg atattgccgc
    19261 cggaaataat actgacggtt tttctgtttt gaatatattg gtctaattta ccgcttaata
    19321 atgcagcaca tgccagagcg cctgcgcctt cggtgacgac tttattgcgc tgaattaagg
    19381 caatcatact gtttctgatt tcgtcttcgc tgaccagcac gatgtcatcg actaattcac
    19441 gaacgatttc gtaagttaaa ttacccgggc gggagacatc acaaccatcc gccagggtgc
    19501 cggtagttcg gtgcgtggtt atttctccgg agtggaaaga agccgccatg ccgtgaacgt
    19561 tttcagactg tacgccaata acacgaatgg tcgggttaat agatttaatt gccaccgcaa
    19621 taccagcaat taaaccgcca ccaccaattg gcacaatcac gttatcgaca tcatagagat
    19681 cttccataat ttccagacca atcgttccct ggccagcaat cactttcgga tcatcgtaag
    19741 gtgggataaa aatacggcct tccatttcga caatttcgct cactttagcg atagtgtcgt
    19801 tgaagttatc accatgcaga acgacttctg cggagtagtc gcacgttgcc gctactttgg
    19861 attttggcgc accttttggc atcaccactt taccgtcgat acccagcatc gcgcaggaga
    19921 gcgaaacccc ttgcgcatgg ttgcccgcag aacaggccac cacgcctttg cgtttttccg
    19981 catcggtcag tgaacttaat ttattaaatg cgccacgaat tttaaatgaa cccgtacgct
    20041 gcatattttc aaacttcagg aatatttcac ctttgcaacg ttcactaaaa tagttggagc
    20101 gaggcatgcc tgttttataa attcgcccag ccagtcgttg tttcgcttca ataatatcat
    20161 caatagcaat cggcagatcg tatgtaatat gcattataaa acctcttcgc ctgaattaaa
    20221 tatgtaggta accgtaaccg acacctgcaa aacagacagg tggattattt atgtttaaat
    20281 aaaaacaaat aactaaccaa cttctattaa ttgccttcgt ctacacccat tataagatga
    20341 atactctttg gctaattcca ccaaaaccga tgctgctttt ttaatacgat aatttttcga
    20401 ccataccgcg gcatattgtg ccacaggtaa tgtttcttca accggaatag taataaattg
    20461 attagaacca aaaggtgacg tcatatcaca aggaattaca gttaagaaat cagcattgag
    20521 aacaagatta taaattgtca cgactgagtc ggttttaacg atgttttcaa tactgatgcc
    20581 atttctttgt aacgtagtaa gcagttcgct gtagtacccc atattagttt gtggcaacac
    20641 ccactgttcg ttcttcaacg actccagcgt ggtggtgccg gtgcatgttc gggacttact
    20701 ggctaccagc acaaactcgg actcgaacag cggctcaaca tgtaaatcct gaagcttcat
    20761 ttctgcactt aatgtaccaa tcgcaaaatc cagtcgaccg tcgcggattg ccggtaagaa
    20821 cgaagacagt tgcgcttcat acatagaaac ctgcgctttc gggaacacct ctttgaactt
    20881 gttgatcatc cctgacataa aagtaaaacc aatcaatgaa ggaaaaccaa atgagacttc
    20941 caccaccgcc tcagaagaca taccgcttat ctcattaacc atatttttca tttcacgggt
    21001 aatggattcg gaacgggaga gtaacaattg accggcaggt gttaatgtta caccggtatt
    21061 tttccgcacc actaattcca caccaaaata atcttcaata tcgttaatga ttttactgac
    21121 ggccggttga gttaacccta attcttttgc agccgagccg atagagccac ttctaatgac
    21181 ttcctgaaag actaccaggt gctgcgtttt cggaagaaga atagtgctca taacgaccta
    21241 cgttaattac ctcattgacg gcatgaagtg tatcaaaatg aaatgaacag gatatgtgcg
    21301 accactcaca aattaacttt caatactttc cagggtatcg tcattacaat taaatacctt
    21361 taaatatcaa caagttaaag cataaaaatc agcataaacc ctgatttttg tcaaaaaaaa
    21421 tgacggggat aaccatattt tatggcgata acatcattcg ttatgtggtt aatacaaaaa
    21481 aggggctgag accatatttc aagcaattac cggataatta ccaggcaaaa tttgagttaa
    21541 atttaatgag caagttctca aattttatta taaataaacc attttcagcg ataaatactg
    21601 cggcacgtca cattttttca cgctatttgt tggagaacaa acatttattt tatcaatatt
    21661 ttaaaatttc gaatacaggt attgatcatc tcgaacaatt gattaacgtc aactttttct
    21721 cttctgacag gacgtcattt tgtgaatgca atcgttttcc ataaattctt ctcccctcat
    21781 aggcgacgaa tagcattttg tgttgaggat cacaaaacga ataattgctg atcgccgcga
    21841 taaggtcaga caaagacaac aagggaaatt ttcacagagc ttttgatcgt cgtaggccac
    21901 agaatgttgc ttcgtttact gtaacgccgg gtaaatgagc gttttttgat agtgcgaaag
    21961 aaccctgcga ggaaaataag catctattat tgtttgtcat gacgacaagg caggcatcaa
    22021 tgaaaggctt cccaattgca catatttttc acccttcaat cccgccaatg cacgcagtgg
    22081 ttaacaatca caatagaaat attgattatt ggacggtaaa aagaaagttt gcag
//
LOCUS       DAJTZL010000057        22053 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.057, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000057 DAJTZL010000000
VERSION     DAJTZL010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22053
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.057"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..180)
                     /locus_tag="OO544_001891"
     CDS             complement(<1..180)
                     /locus_tag="OO544_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR6767501.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKNVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISG"
     gene            complement(177..1244)
                     /locus_tag="OO544_001892"
     CDS             complement(177..1244)
                     /locus_tag="OO544_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR6767502.1"
                     /translation="MDKSKRHLAWWVVGLLVVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQSELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELSWPDDLVVRLPQ"
     gene            complement(1610..3232)
                     /locus_tag="OO544_001893"
     CDS             complement(1610..3232)
                     /locus_tag="OO544_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HCR6767503.1"
                     /translation="MKIGTVAGTNDSTTTITTNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIQDDKISFTIQTNHSEKYSMLSGMRAHILRR
                     NNCYQFIVTINSKNYGCPLDNTDINWCSIVYLLNNMTVNDNANDVAVTESYKPIWNWK
                     ISQYNVFDIKFETMIKPQFADRTYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFAT
                     GHLGIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDTNVNIIISILNRRDIVLESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGDKFIIDLLKRIMSINENVHVLAGNHETNC
                     NGNYMQNFTRMKPLDEDTYSGIKDYPVCFYDPKYKIMANHHGITFDDQRKRYIIGPIT
                     VSSDEMTNALDPVELAAIINKKHHAVINGKKFKNSRAISCRSFNRYFSVSTDYRPKLE
                     ALLACSQMLGINQVVAHNGNGGRECIGETGTVLGLNARDSKHAGRMFSMHNCQINPGA
                     GPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            complement(3494..5101)
                     /locus_tag="OO544_001894"
     CDS             complement(3494..5101)
                     /locus_tag="OO544_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000643692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HCR6767504.1"
                     /translation="MKVFHNIFKHFSSNHQDKHSDKVNSHQHHGKVDKKYRSNTVEVN
                     MLNDFSQIDNDLGLNIIYFLQHGHWRVNEHSLQMEKIWFYNSEPSIDIQEYNSFADNT
                     TNTFIFTIIPDNNHVIKLSSPITVKIECKDGYYFINFSGDKSDIIYKSDITYKVNGLS
                     IIPENFFTLLSGNFKADWRWDVSKETFPKDKFDSYVKSVFSKIDFYKQCGVINPQNAN
                     TAYFGDTDGRVGAVLYALLVSGHIGIREKGWSLLCELLKHENMASSAYIHKNNKLLYN
                     LLNTRDMILNELHQHVFLKDDAITPCIFLGDHTGDRFSTIFGDKYILTLLNSMRNMDG
                     NKDSRINKNVIVLAGNHEINFNGNYTARLENYKLSAGDTYNLIKTLDVCHYDSETKVL
                     TSHHGIIRNEENECYCLGALQVPFNQMKDPIDPEELANIFNKKHKQHMDDRLIHLIRS
                     NAVASTPVYENYFNNTTAFRPKPEDIFKCGQTLKKTKQKYGHYGLGVDQHQKIDNYTM
                     GLNSWKIAPNERGDKKGVPGLSCFQPQ"
     gene            5501..6484
                     /locus_tag="OO544_001895"
                     /pseudo
     CDS             5501..6484
                     /locus_tag="OO544_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 family protein"
     gene            complement(6800..8002)
                     /locus_tag="OO544_001896"
     CDS             complement(6800..8002)
                     /locus_tag="OO544_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCR6767505.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAEDETGFTLDLNGMTVGCEKLVMATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGIVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPNVSLKQLNVRDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLIAAKSS"
     gene            8234..9733
                     /gene="pitA"
                     /locus_tag="OO544_001897"
     CDS             8234..9733
                     /gene="pitA"
                     /locus_tag="OO544_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HCR6767506.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            complement(9977..10312)
                     /gene="uspB"
                     /locus_tag="OO544_001898"
     CDS             complement(9977..10312)
                     /gene="uspB"
                     /locus_tag="OO544_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HCR6767507.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            10703..11137
                     /gene="uspA"
                     /locus_tag="OO544_001899"
     CDS             10703..11137
                     /gene="uspA"
                     /locus_tag="OO544_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HCR6767508.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            11455..12924
                     /gene="dtpB"
                     /locus_tag="OO544_001900"
     CDS             11455..12924
                     /gene="dtpB"
                     /locus_tag="OO544_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HCR6767509.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGMSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADRYGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFKPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            complement(12973..13725)
                     /gene="rsmJ"
                     /locus_tag="OO544_001901"
     CDS             complement(12973..13725)
                     /gene="rsmJ"
                     /locus_tag="OO544_001901"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HCR6767510.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(13733..15775)
                     /gene="prlC"
                     /locus_tag="OO544_001902"
     CDS             complement(13733..15775)
                     /gene="prlC"
                     /locus_tag="OO544_001902"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HCR6767511.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTQALNDCRENVARVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAGLAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELEQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKSVNGLFEVVKRIYGITAKERKEVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            15978..16820
                     /gene="rlmJ"
                     /locus_tag="OO544_001903"
     CDS             15978..16820
                     /gene="rlmJ"
                     /locus_tag="OO544_001903"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HCR6767512.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            16892..18244
                     /gene="gorA"
                     /locus_tag="OO544_001904"
     CDS             16892..18244
                     /gene="gorA"
                     /locus_tag="OO544_001904"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HCR6767513.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(18298..18381)
                     /locus_tag="OO544_001905"
     CDS             complement(18298..18381)
                     /locus_tag="OO544_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Damage inducible protein"
                     /protein_id="HCR6767514.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            18409..18582
                     /locus_tag="OO544_001906"
     CDS             18409..18582
                     /locus_tag="OO544_001906"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6767515.1"
                     /translation="MHPLTHPLPVTAHVSLLDDDYLTPARASVNGTTRTSDQDFESVY
                     AHYQSENASELTG"
     gene            19121..19474
                     /gene="arsR"
                     /locus_tag="OO544_001907"
     CDS             19121..19474
                     /gene="arsR"
                     /locus_tag="OO544_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HCR6767516.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKVQAIVRN
                     LARQNCSGDSKNICS"
     gene            19528..20817
                     /gene="arsB"
                     /locus_tag="OO544_001908"
     CDS             19528..20817
                     /gene="arsB"
                     /locus_tag="OO544_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HCR6767517.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            20830..21255
                     /gene="arsC"
                     /locus_tag="OO544_001909"
     CDS             20830..21255
                     /gene="arsC"
                     /locus_tag="OO544_001909"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HCR6767518.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(21389..21685)
                     /locus_tag="OO544_001910"
     CDS             complement(21389..21685)
                     /locus_tag="OO544_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6767519.1"
                     /translation="MQLEEVAKALKELGHPTRLFIFKHLVKAGEQGLPVGELQKQLGI
                     PGSTLSHHISALVSVGLVKQNRESRTLMCVSQYAMLEAIIEFLREECCVNSKIA"
     gene            21792..>22053
                     /locus_tag="OO544_001911"
     CDS             21792..>22053
                     /locus_tag="OO544_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCR6767520.1"
                     /translation="MSSWLAMLQDAAEMFVFLAVELSLLFIVISAGVSLIRQKVPDHK
                     IQQMMGARKGRGYLLAALLGAVTPFCSCSTIPMLRGLLSAKAG"
BASE COUNT         5353 a         5526 c         5277 g         5897 t
ORIGIN      
        1 accggaaatc aacgacaata agctcgactt cccgacgcca tccggaccaa tcagcccgac
       61 catacagcgg gccggaatat cgagagtaat attgttcagc gcaacgtttt ttccataatg
      121 ctggctcacg cccgccagtt gcgcgacagg cgggacggga accagttcca gatgcgtcat
      181 tgcggcaacc tcaccacgag gtcgtcaggc cacgaaagtt cttcattcac ccgcacccac
      241 gctacgcccg gtaaaccggt tttgacatat tccagatgct gctggagtaa ttccggtggg
      301 atacgcgctt tgacgcggaa catcagtttc agccgttcat cgctggtttc gacggttttt
      361 ggcgtgaact gggcgacact ggcgacaaaa ctgatggtcg ccggaatacg cagatctggc
      421 gcggcatcga ggatcagccg ggcttcaccg ccaagtttca gcgtgcccgc ctgttcggtt
      481 ggcaggaaga aagtcatata gacgtcgctg agatcgacca tattcagcac ccgaccgcct
      541 gccgccagca cttcccctgg ctcagcaacc cgatactgca cgcgtccgtc acgcggggct
      601 ttcagttcac tgtcatcgat atctgcggca atacgccgtt cagtggcttg cgcggcttcg
      661 acgcgagttt gggcctgaat gatattggta cgggccgctt ctatagccgc tttagaagcc
      721 gatacctgag cttttgccga ttccagcgca gctcgggcgc tctcagcggc ggcgcgatcg
      781 tcatccagct gttgcgcaga aatagcccct cgttgggcga gcgagcggga acgcgtatga
      841 cgtttagcca cggagtccag ttcggactgg cgttgattaa ccagcgactg cgcggcacga
      901 gtttcgcttt gccgttgctc cagcaaagcc tgcgcggcag caacggcact ttgtgcttct
      961 ttgatttgcg cgatggcttc cagtcgctgc tcctgcaaca cgcgagtatc catcttcgcc
     1021 agcacttcgc cttcgcgaac aaactggcct tctttcacca gaatggtgtc gatacgcccg
     1081 gcaattttgc tggcgatatc cacttccgtc gcttcaatgc gcccattgct gacagcaaag
     1141 ccttccggca cacccgccgg gcgcaacagc caccacgcca cgacggcagc caccaccagt
     1201 aacccgacaa cccaccacgc cagatggcgc ttactcttat ccataatcga cccgccataa
     1261 tccctgcaag caaacgacgc gtgttacaac tgccgccgaa cgacgttcag cggattcttc
     1321 catccgggat aagaaggaaa cgacaaatac gaccagcgcg cagagcattg agcgccaacg
     1381 gggaaggctt agtgaaaacg ggtagtaatg ttgtcatcat cagcattcct ggccgtaaat
     1441 gaaacacgca tactcataag tgtgatctat aaaactggct gacaaaacgg tatccatcct
     1501 gttggatagc gtttcacgcc tgttcagttg caggaacaga gcattccttt ttacgtttta
     1561 tcaacagtct caaccagata gtaacattaa tattcatgtt gtctatggtt cagagctgta
     1621 acattgtgcg ccttctgatc aaaggcataa gtccatttct gtttttttca tgctggtaag
     1681 acttccaggg agtggttatc tcaggccctg caccagggtt aatttggcaa ttatgcatgc
     1741 tgaacattct tccagcatgt ttactgtcac gggcgttaag acctaaaact gtccccgttt
     1801 cacctataca ttctcttcca ccattaccgt tatgtgcaac aacttgattt atacctaaca
     1861 tttgtgagca tgctaatagt gcttcaagtt taggtctgta atctgtcgaa acagaaaaat
     1921 aacgattaaa ggatcgacaa gagatagccc ggctattttt aaactttttg ccattaatta
     1981 cagcatggtg ctttttattg ataattgccg ctaattcgac cggatcgaga gcattggtca
     2041 tttcatcact agagactgtt atcggtccta taatgtaacg ttttcgctga tcatcaaaag
     2101 ttatcccatg atgatttgcc attattttgt atttggggtc atagaaacat acaggataat
     2161 cttttatccc agagtaggta tcctcatcaa gtggtttcat gcgcgtgaag ttctgcatat
     2221 agttaccatt gcaattcgtt tcatgatttc cagctaatac atgaacgttt tcattgatac
     2281 tcattatgcg ttttaataaa tcgatgataa atttatcgcc acagatatta ctaaatcgat
     2341 cgccagtctg gtcgcccaga aacgtacatg gcgtgactgc gtctttatta ataaccaaat
     2401 aaggctgaag actttctaaa acaatgtctc ttctgttcag gatactgatt attatattaa
     2461 cattagtgtc aagttgctca ttaaagttct cccttagcaa tacgttgaga acttcatctt
     2521 ctatattaag caactggcta aggaaatttt ctccttcagc catgattccc aaatgtcccg
     2581 ttgcaaaaag cgcgtacaaa actgcaccga ctgagccatc ggtatcgcca aaataggtcg
     2641 gtctggtact tgtgggatca acaggtgaac aattggagaa ataggttctg tcagcgaact
     2701 ggggttttat cattgtttcg aatttaatat caaatacatt gtattgcgat attttccagt
     2761 tccagatggg tttgtacgac tcagtaactg caacatcatt agcattatca tttactgtca
     2821 tattattaag taaataaaca atgctgcacc aattaatatc ggtattatca agagggcaac
     2881 cgtaattttt actattaatt gtcacaataa actgataaca gttattccta cgtagaatat
     2941 gagcgcgcat gcccgatagc atactatatt tttctgaatg attcgtttgt atggtaaatg
     3001 aaattttgtc atcttgaata gattgttgga accgttctat ttgccaatca tctatttttt
     3061 ccacaacctc accgagcatt gtcctggcaa tacttgatat atcatcacct ataccatttg
     3121 caatataccc aaacagttct ttagtgaagt tggtaacatg ctcttgcacc atatcatttg
     3181 ttgtgattgt cgttgtactg tcgttagtgc ctgccacagt tccaattttc atcttttacc
     3241 tcatattatt taccgcgagg aatacatagt taacatttaa tcttatgaca tacacctcat
     3301 gttcgatggg aaaataatta tattttgcac aaatcattca gccttaaagc ataatatgaa
     3361 atttcttatc tcatagcaga gaaagtccct aaagattgta gaggcgtcat cagaacatgc
     3421 ctctacaatc gattaagaaa acctatgaaa tactggcaaa attaaaaaga gataccacag
     3481 cactcatgaa gttttattga ggttgaaaac aactaagccc tgggacacct tttttatcac
     3541 cacgttcgtt aggtgctatc ttccaggaat ttagccccat ggtataatta tcaatttttt
     3601 gatgctgatc tacccctaat ccataatggc catatttctg tttagttttt ttgagcgtct
     3661 gtccacattt gaagatatct tcaggttttg gtctgaaagc agtagtgtta ttaaaataat
     3721 tttcatatac aggtgtggat gctacagcat tgcttctaat tagatggata agacgatcat
     3781 ccatgtgttg tttatgtttt ttattaaata tatttgctaa ttcctcagga tcaattgggt
     3841 ctttcatctg attaaatgga acctgcaaag caccgagaca ataacattca ttttcttcat
     3901 ttcttattat tccgtgatga ctggttaaaa ctttcgtttc tgagtcataa tgacaaacat
     3961 cgagcgtttt aattaagtta tacgtatcac cggcagagag tttataattt tctagtcgtg
     4021 cagtatagtt accattaaaa ttgatttcat gatttccagc cagaacgatg acatttttat
     4081 taatacggct atctttatta ccatccatat ttcgcataga atttagcaaa gtaaggatat
     4141 atttatcacc aaaaatagta ctaaatctat cgcctgtatg atccccaagg aagatacaag
     4201 gggttatcgc atcgtccttt aaaaaaacat gttgatgcaa ctcattcaat atcatgtctc
     4261 tcgtatttaa taaattgtag agcaatttat tatttttatg tatgtatgca gatgaagcca
     4321 tgttttcgtg ttttaataat tcacataata aactccaacc cttttccctg atgcctatat
     4381 gccctgaaac gagcaatgca taaagtacag cgcctactct tccatctgta tcgccaaaat
     4441 aagccgtgtt ggcattttgt gggttaatca caccacattg tttataaaag tctattttag
     4501 aaaacacaga tttaacatag ctatcaaact tatccttagg aaacgtctct ttagagacat
     4561 cccaacgcca gtcagcttta aaattaccgc taagaagagt aaagaaattt tcaggtatga
     4621 tagaaagccc attaacttta taagttatgt ctgatttata aattatgtct gatttatctc
     4681 cggagaaatt aataaaataa taaccatcct tacactcaat cttaactgta atgggagacg
     4741 ataattttat cacatggtta ttgtctggta taattgtaaa gataaacgtg ttagtagtat
     4801 tatcggcgaa actattatat tcttgaatat ctatagatgg ctcactattg taaaaccata
     4861 ttttctccat ctgaagacta tgttcattta ccctccagtg accatgttgc aggaaataga
     4921 tgatattcag gcctaaatcg ttatctattt gcgaaaagtc attcagcata ttaacttcaa
     4981 ctgtgtttga tcgatatttt ttatcaacct tgccatggtg ttgatgactg ttaactttat
     5041 ctgaatgctt atcttgatgg tttgaagaga agtgcttaaa tatattgtga aatactttca
     5101 taaataacct gtaatatttt tacttgttag taacaaaata aacaatacat gtctgaaaga
     5161 catatttacg atgatacaaa gcaataatac ttatatgttc gatgatttta agcaccgaga
     5221 ctatccggtc ttttactaaa taatggaaaa caatccatcc ctggaagata attgtaaaaa
     5281 aaacaacaga ataagaatta atcttaggat aaatatttat ttatcatggc cttttaaccg
     5341 gtaaaaaatg gcactcggca cagtaaaccc cgctatagtt atgctatgca tttgtcttta
     5401 atgatatctc gtcgttatat taacaggctg aaattatcat taatgcacat ttcattatta
     5461 gcagggatac ctgaggagtt taaagtgaac atatatatcg ggtggctttg tgctggaaag
     5521 ttcagggcaa agcgagtatt tcgtcgcggg tcatgtgctg atttctctgg cggccatatg
     5581 cctggcatta ttcactaccg catttattat catttcgcag ctcacgcgcg gcgttaatac
     5641 gttttacaat acattgttcc ccattattgg ctatgcgggg tcaattatca ccatgatatg
     5701 gggttgggca ctgttagcag gcaatgatgt gatggcagac gagtttgtcg ccggccatgt
     5761 tattttcggc gttggtatga ttgccgcctg tgtatcgacg gtggcagcgt catccggtca
     5821 ctttctgctc attcccaaaa atgcagcggg gagcaagagc gatggaacgc cggtacaggc
     5881 ttattcttca ttaatcggta attgcctcat tgccgttccc gttttactca ccctgctcgg
     5941 tttcatttgg tctattacgc tgttacgtag tgctgacata actccgcatt atgtcgcggg
     6001 tcacgtattg cttgggttaa ccgcaatctg tgcctgtcta attggccttg ttgccacaat
     6061 tgtccatcaa acacgtaata cgttttcaac taaagaacac tggctgtggt gttattgggt
     6121 tatttttctc ggctcaatca cggtactgca ggggatatac gtcttagtca gttccgatgc
     6181 aagcgcccga ctggctcccg gcattattct tatttgcctc ggaatgatct gttacagcat
     6241 attctcaaaa gtctggctac tggcactggt ctggagacgt acctgttcgt tagccaacag
     6301 aataccgatg atacccgtct tcacctgcct gttttgcctt ttcctggcat catttcttgc
     6361 ggaaatggcg cagaccgaca tgggatattt tattccttcg cgagttctgg tcggtttggg
     6421 agcggtatgc tttacgttgt tctcaatcgt ttcaatatta gaagcgggtt ctgctaaaaa
     6481 ataattgcaa cgtaccggat aaaaccagcg ttgaccattt gcgtaacgct ggtttttctt
     6541 aggcatcatg aaataacgca gcattaatgc atagtggtta aggataaaaa aacaacaaag
     6601 ttactgtctt tttcagcctg ttcatatttt ataaagatct ggccactggg tgagttttca
     6661 atcacctttc cttccacctt atattaagcc tggagggttt cagttcacgg gctcattaga
     6721 aaataatcac aagatagcca ctatcaaaat tagctcattt ttaatgcggc tctaataatt
     6781 ttcatcttta ggtaacaggt caggacgact tcgctgcaat caaatcctgc gcacaagccc
     6841 acgcactcga ccacgcccac tggaagttat agccccccag ccagccggta acgtccatca
     6901 cttcgccgat gaagtacagc ccaggcactt tgcgcgcttc catcgtccgt gaagagagtt
     6961 cgttggtgtc cacgccgccg agcgtcactt cggcagtgcg atagccttca gtgccgttgg
     7021 gttgtacacg ccagtcggtc aatgtgctaa tcagtgcctg ttggtcacgc acgttgagct
     7081 gttttagcga aacattcggg atttgcccga gttgctgtaa gcgttcaacc aaccgcttcg
     7141 gtagatgaac cgccagtgtg tttttcaggc tctgattcgg atgtgcgtta cgctgctcat
     7201 tcaggaaggt ttcgagatcc acatccggta gcagattgat actgacaaat tcacccggtt
     7261 gccagtagct ggaaatctgc aacaccgccg gtccagacaa gccgcgatgg gtgaagagta
     7321 agttctcacg gaaaacgatg ccgttttcag cggtaatcac ggaaggcacc gccacgcccg
     7381 ccagcacctg taactcttcg agcaacggtt tatgcagagt gaatggcacc agacccgcac
     7441 gggtcggcag cacgttgagg ccaaattgtt cggcaatctt ataaccaaac ggcgacgcgc
     7501 ccagccccgg cattgacagc ccaccggtcg ccatgaccag cttttcgcaa ccgacagtca
     7561 tgccgttcag atcaagcgtg aagcctgttt catcctccgc cacactcagc acttcgctac
     7621 gcaatctgaa ggtcacattg cccttctcgc actcatccac cagcatgtcg acaatctgct
     7681 gcgcggagtc atcgcagaag agttgcccta acgttttctc gtgccatgcg atgccgtgtt
     7741 tattgaccag atcaatgaaa tcccactggg taaaacgtgc gagtgcagac ttacaaaaat
     7801 gcggattctg gctcagataa gcgcctggtt cgacataaag gttggtaaag ttgcagcgcc
     7861 caccgccaga cataaggatt ttgcgccctg gttttttacc attatcgatc agcagaaccc
     7921 ggcgtcctgc ctgacctgcc agcgcagaac agaacatacc cgccgcacca gcgcctataa
     7981 taatggcatc aaacctttcc acgttgcgct cctcttagaa aaaacgggcg tgaattgtaa
     8041 agattcctca gtggtcgcac cagcatcaat attactaaaa ggaagtattt gcctgaatta
     8101 tataagataa ttattttttg agtgaaatcc atacaggagg caaatcaaaa aaagtctata
     8161 tttcactttg cccgcgccgc gaaagtcact gataatgcgc cgcgttcatg tcctcaaaat
     8221 ggcgtaacgt cctatgctac atttgtttgc tggcctggat ttgcataccg ggctgttatt
     8281 attgcttgca ctggcttttg tgctgttcta cgaagccatc aatggtttcc atgacacagc
     8341 caacgccgtg gcaaccgtta tctatacccg cgcgatgcgt tctcagctcg ccgtggttat
     8401 ggcggcggtg ttcaactttt tgggtgtttt gctgggtggt ctgagtgttg cctatgccat
     8461 tgtgcatatg ctgccgacgg atctgctgct taatatggga tcgtctcatg gccttgccat
     8521 ggtgttctct atgttgctgg cggcgattat ctggaacctg ggtacctggt actttggttt
     8581 acctgcatcc agctctcata cgctgattgg cgcgatcatc gggattggtt taaccaatgc
     8641 gttgatgacc gggacgtcag tggtggatgc actcaatatc ccgaaagtat taagtatttt
     8701 cggttctctg atcgtttccc ctattgtcgg cctggtgttt gctggcggtc tgattttctt
     8761 gctgcgtcgc tactggagcg gcaccaagaa acgcgcccgt atccacctga ccccagcgga
     8821 gcgtgaaaag aaagacggca agaaaaagcc gccgttctgg acacgtatcg cgctgatcct
     8881 ttccgctatc ggcgtggcgt tttcgcacgg cgcgaacgat ggtcagaaag gcattggtct
     8941 ggttatgttg gtattgattg gcgtcgcgcc agcaggcttc gtggtgaaca tgaatgccac
     9001 tggctacgaa atcacccgta cccgtgatgc catcaacaac gtcgaagctt actttgagca
     9061 gcatcctgcg ctgctcaaac aggctaccgg tgctgatcag ttagtaccgg ctccggaagc
     9121 tggcgcaacg caacctgcgg agttccactg ccatccgtcg aataccatta acgcgctcaa
     9181 ccgcctgaaa ggtatgttga ccaccgatgt ggaaagctac gacaagctgt cgcttgatca
     9241 acgtagccag atgcgccgca ttatgctgtg cgtttctgac actatcgaca aagtggtgaa
     9301 gatgcctggc gtgagtgctg acgatcagcg cctgttgaag aaactgaagt ccgacatgct
     9361 tagcaccatc gagtatgcac cggtgtggat catcatggcg gtcgcgctgg cgttaggtat
     9421 cggtacgatg attggctggc gccgtgtggc aacgactatc ggtgagaaaa tcggtaagaa
     9481 aggcatgacc tacgctcagg ggatgtctgc ccagatgacg gcggcagtgt ctatcggcct
     9541 ggcgagttat accgggatgc cggtttccac tactcacgta ctctcctctt ctgtcgcggg
     9601 gacgatggtg gtagatggcg gcggcttaca gcgtaaaacc gtaaccagta ttctgatggc
     9661 ctgggtgttt acccttccgg ctgcggtact gctttccggc gggctgtact ggctctcctt
     9721 gcagttcctg taatcgtacg caccaaaacg agcgggtcag ctggcccgct tcagattgtg
     9781 acatagtgcg ctttgttcat gccggatgcg gcgtgaacgc cttatccggc ctacaaaaac
     9841 atgcaaattc aatagattgc agagattatg taggcctgat aagcgtagcg catcaggcaa
     9901 ttttgcgttt gtaatcagtc tcgagcgggt cagtttactg gcccgctttt tttatgcgtt
     9961 attattgctg aagtgcttaa tgccaaatca tcaatgcaat caggctgacc accaccagac
    10021 cacacaatgc gctggtcaga ataaactgcc gacgcacccg ctcacagcgg cgaataaatt
    10081 catcatcgtg atgatcgcga taacgttggg catagatata ccaaacgaga cgcacctgtt
    10141 tgttgggttg gccatgtgag gtaaaaaagc cccctccatc aacatattga tagagcaatg
    10201 gatcgcagtt acgcagtacc actaacaacg cgcgtagtga tgagaaatag cgcgccatgt
    10261 taacaatgca aacgacacat aaagcccaaa ataatgcgac ggtgcttatc atacctcctc
    10321 cccggcgacc tgcccgcgga gttccacccc ggggctaccg ctcccgatac gctgccaatc
    10381 agttaacacc aggtcctgga gaaaccgctt ttgtggtgac caacatccga gcggctctat
    10441 agatagtgta ggagatcagg ttgttttttt tccagaaggt taaccactat caatatattc
    10501 atgtcgaaaa tttgtttatc taacgggtaa gcaaggcgga ttgacggatc atccgggtcg
    10561 ctataaggta aggatggtct taacactgaa tctttacggc tgggttagcc ccgcgcacgt
    10621 agttcgcagg acgcgggtga cgtaacggca caagaaacgc tagctggcca gtcatcgaca
    10681 actttatgga aggagtaaca ctatggctta taaacacatt ctcatcgcgg tcgatctctc
    10741 cccggaaagc aaagttctgg tagagaaagc agtctctatg gctcgcccct acaatgcgaa
    10801 agtttctctg atccacgtag atgtaaacta ctctgaccta tacaccgggc ttattgatgt
    10861 gaatctgggt gatatgcaga aacgcatctc tgaagagaca catcatgcgc tgaccgagct
    10921 ttccactaat gcaggctacc caatcactga aaccctgagc ggcagcggcg acctgggcca
    10981 ggttctggtc gatgcaatca agaaatacga tatggatctg gtggtttgtg gtcaccacca
    11041 ggacttctgg agcaaactaa tgtcttccgc acgtcagctg atcaacaccg ttcacgttga
    11101 tatgctgatt gttccgctgc gcgacgaaga agaataatca tccccccacg acgtgttcct
    11161 gaacgcccgc atatgcgggc gttttgcttt ttggcgcgcc ttgttacctg atgcagcgta
    11221 aaccccttat ctggcctacg atctgcgtac gcaatcaaaa tctcctgcca atacaacatt
    11281 taacactatc atattttcca tcattagtgt gatcagctgg ttattttctg ttgtaatagt
    11341 gtattaatct attcaccgca tcaatattaa gaatctctga cagatgtaaa ctttttcgcg
    11401 cgttatccct tacgcgttca tacttttcag gatggtattg gaaggttaat aaatatgaat
    11461 acaacaacac ccatggggat gctgcagcaa cctcgcccat ttttcatgat cttttttgtc
    11521 gagttatggg agcgattcgg ctactacggc gtgcagggcg tactggcggt tttcttcgtt
    11581 aaacagcttg gattctcgca agagcaggct tttgtcactt ttggtgcttt tgctgcgctg
    11641 gtctatggcc tcatttccat tggcggctat gtcggcgacc acctgctggg gaccaaacgc
    11701 accattgttc tcggagcact tgtgctggcg attggctact tcatgaccgg catgtcgcta
    11761 cttaagcctg acctgatttt catcgccctg gggactatcg ctgtcggtaa cggcctgttt
    11821 aaagctaacc cagccagctt gctttcgaag tgctatccgc cgaaagatcc gcggcttgat
    11881 ggcgcattca ccctgttcta tatgtcgatc aatatcggct cgttgatagc gttatcgctg
    11941 gcccctgtga tcgctgatag atacggttat tcagtcacct acaacctgtg cggggcgggg
    12001 ttaattatcg cattactggt ttacatcgcc tgtcgtggaa tggtgaaaga cattggttct
    12061 gaacccgact tcaagccgat gagcttcagc aaactgttgt acgtattact tggcagcgtg
    12121 gtgatgatct tcgtatgtgc atggctgatg cacaacgtag aagtcgccaa tctggtgctg
    12181 attgttctct ccatcgtcgt caccatcatc ttctttcgtc aggcattcaa gctggataaa
    12241 accgggcgca ataaaatgtt tgtcgccttt gtcctgatgc tcgaagcggt ggtgttttac
    12301 attctctacg cccagatgcc aacgtcgctg aacttctttg ccatcaacaa cgtgcatcat
    12361 gaaattctcg gcttttccat aaacccggtc agcttccagg cgcttaaccc gttctgggtg
    12421 gtactcgcca gcccaatact ggcaggcatt tacacgcatc tgggtaacaa aggcaaagac
    12481 ctctcgatgc cgatgaaatt tactctcggc atgtttatgt gctcactggg ctttttgacg
    12541 gcggcagctg cgggaatgtg gtttgcggat gcacaagggc tgacatcgcc atggtttatc
    12601 gtgctggtgt atttattcca gagcctgggt gaattgttta ttagcgccct tggcctggcg
    12661 atgattgctg ccctggtgcc gcagcatttg atgggcttta ttctcgggat gtggttcctg
    12721 acgcaggctg ccgcattctt gctgggcggc tatgtggcaa catttaccgc agtgccggac
    12781 aacattaccg atccgcttga gacgttaccc gtctatacca acgtgtttgg taagattggc
    12841 ctggttacgc tgggcgttgc agtagtgatg ctgttgatgg tgccgtggct gaaacgcatg
    12901 attgcgacgc cggaaagcca ttaattattc ttgcagaaag cagggtagcg ttatcgctac
    12961 cctgctttac ttttacacgg gcgtacctgc atagatatca aagcgatgcc ctttagtcac
    13021 aaccgcgttt ggcgtggcga cattcgctag cggtggcgcg tagtccgggc gctttactac
    13081 cacgcgtttg gtcgccagca agcgtgcagg ctccagcaat ccatcggcat caagatccgg
    13141 ccccaccagc gactgaaaga cacgcatctc ttttttcacc agcgcgcttt tctgcttatg
    13201 cgggaacatc gggtcgagat agaccacctg cgggcgtggg gtaatatcgg ttagcgccgt
    13261 caggctggag gcgtgaatta actgcaaacg ctcctgcaac cagccgccga tttccgcatc
    13321 cgcataacca cgcgccaggc cgtcgtcgag cagcgcggca acaactggat tacgctccag
    13381 catccgcacg cggcaaccga ctgaagccag cacaaaggca tcgcgcccca gtcctgcagt
    13441 ggcatccacc acatccggca aataatcgcc tttaatgccc accgctttcg ccaccgcctc
    13501 accgcgacca ccgccgaatt tgcgtcggtg cgccatcgct ccgccaacaa aatcaacaaa
    13561 gatgccgcca agttttggct catcacgctt gcgcaattcc agatgttccg gcgttaacac
    13621 cagcgccatc aggttgtctt catcgtgctc cagcccccag cgggccgcca gaacagataa
    13681 ggcgccgtct ccggcgcctg tttcatcaat taagcagatt ttcactgaat gatcagccct
    13741 taatgccgta atgctccagc atcgcatcca gctgcggttc acgaccacgg aagcgtttga
    13801 acagatccat cggctcttct gaaccgccac ggctcagaat gttgtcgagg aacgactgcc
    13861 cggtttcacg gttgaaaatg ccctcttcct caaagcgcga gaaagcatct gccgccagta
    13921 cgtcagccca caggtagctg tagtaacctg ccgcataacc accggcgaaa atatggctga
    13981 aagcgtgcgg gaaacggccc caggacggag atggcaccac ggcaaccagt ttcttgattt
    14041 ctgccagagt ttcgaggatt tttgccccct gatccgggcg gaactcggca tgaaggcgga
    14101 aatcaaacag gccgaactcc agctgacgca gaataaacag cgccgcctgg tagttcttcg
    14161 ccgccagcat tttatccagc aactctttcg gcagcggttc gccggtttca tagtgaccag
    14221 agataaacgc cagcgcctcc ggctcccagc accagttttc cataaactga ctcggcagtt
    14281 cgaccgcatc ccacggcaca ccgctgatac cggaaacacc agcggtttcg atgcgggtca
    14341 gcatatggtg caggccgtga ccgaactcgt ggaacagggt gatcacttcg tcgtgagtga
    14401 acagcgccgg tttaccattt accgggcggt tgaagttaca agtcaaatac gcgaccggtt
    14461 tttgcagaga accatcagct ttacgcatct ggcctacgca gtcatccatc cacgccccgc
    14521 cgcgcttgtt ttcacgggca tacagatcga ggtagaagct gccgcgcagt tcgttatttt
    14581 cgtcatacag ttcgaagaaa cgtacatccg gatgccagac atcaacctct ttacgctctt
    14641 tagcggtgat gccgtaaata cgtttcacca cttcaaacag gccgttaacc gatttgtttt
    14701 ccgggaagta cggacgcagc tgctcgtcgc tgatgctgta gaggtgctgt ttctgttttt
    14761 cgctgtagta agcgatatcc cacggctgca actcatcgac accaaattcg gctttagcga
    14821 aggcacgcag ttgctccagc tctttttcgc cttgcggacg cgcgcgtttt gccagatcgg
    14881 ttaagaaatc cagcacctgc tgcgggtttt ctgccatttt agtggcaagg gatttaaagg
    14941 cgtagttttc aaagcccagc agttgcgcca gttcgtgacg cagcgcgagg atctcttcca
    15001 tcaccttgct gttatcccac ttaccggcgt tcgggccttg atcggaggcg cgggtgctgt
    15061 aagcgcgata catctcttcg cgcagagcct ggttgtcgca gtaggtcatt accggcaggt
    15121 agcttgggat atccagcgtc agcaggtagc cttccagctc tttcgcttcg gcctgggctt
    15181 ttgccgcagc cagcgcgctt tctggcatcc ccgccagccc cgcttcgtcg gtaacgagtt
    15241 tggtccagcc catcgtcgca tcgaggacgt tgttgctgta ctggttgccc agttcagaaa
    15301 gacgggtagc aatttcgccg taacgctgct gtttctcttt cggcagacct atgccagaga
    15361 gttcaaaatc acgcagggcg ttatcaaccg ctttcttctg cgccgtgttc agcgtggcgt
    15421 aatgatcgcc atcgcgcagg tcgcggtacg ctttatacag cccttcatgt tgccctaccc
    15481 aggtgctgta ttccgacagc agcggcaggg tttgctcgta ggcttcacgc agttccgggc
    15541 tatttttcac cgagttcagg tggctgaccg gggagaagat acgccccaac acatcgtcca
    15601 cttccgccaa cggctggcag agattttccc aggtgtacgg tgccccttgc gctactacgc
    15661 gcgccacatt ttcgcggcaa tcgttcagcg cctgagtcac ggctggaacg acatgttccg
    15721 ggagaatttt agaaaacgga ggcaattcaa agggagtcag taacggattc gtcattcgcg
    15781 cagtcctggt taaagaggtt aaggaagcgc tcaacaggcg ctttacataa tgtgtgtagc
    15841 atggggttaa gtgtagtgaa tttcaatgag aaacgttacg ctttcgcggc ggcggcctct
    15901 tttcggtata ctgtcctgat acgcttttgt gcgccccgaa tacgggccga tttttactta
    15961 ccggaacacc tttacccatg ctcagttatc gccacagctt tcacgctggc aaccacgccg
    16021 acgtccttaa acataccgtt cagagcctga tcatcgagtc gctgaaagag aaagataaac
    16081 cgtttctcta tctcgatacc cacgcagggg ccgggcgtta tcagttaggc agcgaacatg
    16141 ccgagcgtac cggcgaatat ctcgaaggta tcgcccgtat ctggcagcag gacgatttgc
    16201 ccgcagaact ggaggcgtac atcaatgtgg taaaacactt caaccgtagc ggtcagttgc
    16261 gttactaccc cgggtcgccg ttgattgctc gccagctact gcgcgaacaa gacagcctgc
    16321 aactgaccga actgcacccg agcgattacc cgttgctgcg ttctgaattt cagaaagaca
    16381 gccgtgcgcg cgtcgaaaaa gccgacggtt tccagcagct taaggccaaa cttccgccgg
    16441 tttcccgccg tggtttaatc cttatcgacc cgccgtatga aatgaaaacc gactatcagg
    16501 cggtggtcag cgggatagca gaaggttaca aacgtttcgc cactggtact tacgcactgt
    16561 ggtatccggt ggtgctgcgt cagcaaatta agcgcatgat ccacgatctg gaagcgaccg
    16621 gtattcgcaa aattctgcaa attgaactgg cggtactgcc agacagcgat cgccgtggca
    16681 tgaccgcttc cggcatgatt gtgattaacc cgccgtggaa actggaacag cagatgaata
    16741 acgtgctgcc gtggctgcac agcaaactgg ttccggcagg caccgggcac gccaccgtaa
    16801 gctggatcgt gccggagtaa ttgcagccat tgctggcacc tattacgtct cgcgctacaa
    16861 tcgcggtaat caacgataag gacactctgt catgactaaa cactatgatt acatcgccat
    16921 cggcggcggc agcggcggta tcgcctccat caaccgcgcg gctatgtacg gccagaaatg
    16981 cgcactgatt gaagccaaag agctgggcgg cacctgcgtg aacgtcggct gcgtgccgaa
    17041 aaaagtgatg tggcacgcgg cgcaaatccg tgaagcgatc catatgtacg gccctgatta
    17101 tggtttcgat accaccatca acaagttcaa ctgggaaacg ttgatcgcca gccgtaccgc
    17161 ctatatcgac cgtattcata cttcctatga aaacgtgctc ggtaaaaata acgttgatgt
    17221 aatcaaaggc tttgcccgct tcgttgatgc caaaacgctg gaggtaaacg gcgaaaccat
    17281 cacggccgat catattctga tcgccacagg cggtcgtccg agccacccgg atattccggg
    17341 cgtggaatac ggtattgatt ctgatggctt cttcgccctt cctgctttgc cagagcgcgt
    17401 ggcggttgtt ggcgcgggtt acatcgccgt tgagctggcg ggcgtgatta acggcctcgg
    17461 cgcgaaaacg catctgtttg tgcgtaaaca tgcgccgctg cgcagcttcg acccgatgat
    17521 ttccgaaacg ctggtcgaag tgatgaacgc cgaaggcccg cagctgcaca ccaacgccat
    17581 cccgaaagcg gtagtgaaaa atgccgatgg tagcctgacg ctggagctgg aagatggtcg
    17641 cagtgaaacg gtggattgcc tgatttgggc gattggtcgc gagcctgcca atgacaacat
    17701 caacctagaa gccgctggcg ttaaaactaa cgaaaaaggc tatatcgtcg tcgataaata
    17761 tcaaaacacc aatattgaag gtatttacgc ggtgggcgat aacacgggtg cagtggaact
    17821 gacaccggta gcagttgcag cgggtcgccg tctctctgaa cgcctgttta ataacaagcc
    17881 ggatgagcat ctggattaca gcaacattcc gaccgtggtc ttcagccatc cgccgattgg
    17941 tactgttggt ttaacggaac cgcaggcgcg cgagcagtac ggcgacgatc aggtgaaagt
    18001 gtataaatcc tctttcaccg cgatgtatac cgccgtcacc actcaccgcc agccgtgccg
    18061 catgaagctg gtgtgcgttg ggccagaaga gaagattgtc ggtattcacg gcattggctt
    18121 tggtatggac gaaatgttgc agggcttcgc ggtggcgctg aagatgggcg caaccaaaaa
    18181 agacttcgac aataccgtcg ccattcaccc aacggcggca gaagagttcg tgaccatgcg
    18241 ctaaatgtta aagggctaag agtagtgtgc tcttagccct taattacgtt tccgctatca
    18301 gttcagaagc tgaagcagaa agcggatcag ttccagcagc gcaattaacg ctccaagaac
    18361 gatgattgct ttatcaatca cccgttttct ccatgcgatg gagtgagaat gcatccgctt
    18421 actcatccac tgcctgtcac ggcgcatgtc tcattgttag atgacgacta tctcactccg
    18481 gccagagcat cagttaacgg caccacccgt acttctgacc aggactttga aagcgtttat
    18541 gcgcattacc agagtgaaaa tgcctcagag ctaactggat aatcatacag tacattcagg
    18601 ttataaaacc agcatgtcct tgcaatagtt tcagtatggt attagcattg atgcgttaga
    18661 tgatgactat ctcactccag ccagagccac caactcaggg ctgaagagta aaaaaaccga
    18721 cgcaaagtcg gtttttttac atccggattc ggacaaggct taatatgacg atgacccagt
    18781 gaaagtatat aaatcgtcat tgcgatatat accgaagtgc cccctccgcc agctgaagaa
    18841 atcgctaatt cttgcaatgt tagccactgg ctaatagtat tgaaccgtta gatgtgggca
    18901 ttctcactcc agccagagcc accaactcag gctgaagagt aaaaaaccga cgcaaagtcg
    18961 gtttttttac gtcctgattc aaaccgcctt tcaaatgaat agccaactca aaattcacac
    19021 ctattacctt cctctgcact tacacattcg ttaagtcata tatgtttttg acttatccgc
    19081 ttcgaagaga gacactacct gccacaatca ggagcgcaat atgtcatttc tgttacccat
    19141 ccaattgttc aaaattcttg ctgatgaaac ccgtctgggc atcgttttac tgctcagcga
    19201 gctgggagag ttatgcgtct gcgatctttg tactgccctc gatcagtcgc agcccaagat
    19261 ctcccgccac ctggcattgc ttcgtgaaag cgggctattg ctggaccgca agcaaggtaa
    19321 gtgggttcat taccgcttat caccgcatat tccagcatgg gcggcgaaaa ttattgatga
    19381 ggcctggcga tgtgaacagg aaaaggttca ggcgattgtc cgcaacctgg ctcgacaaaa
    19441 ctgttccggg gacagtaaga acatttgcag ttaaaaattt agctaaacac atatgaattt
    19501 tcagatgtgt tttatccggg aggcattatg ttactggcag gcgctatctt tgtcctgacc
    19561 atcgtattgg ttatctggca gccgaaaggt ttaggcatcg gctggagtgc aacgctcggc
    19621 gcagtactgg cgttagttac gggcgtggtt catccgggtg atattccggt ggtgtggaat
    19681 atcgtctgga acgcgacggc tgcgtttatc gccgtcatta tcatcagcct gctgctggat
    19741 gagtccggct tttttgaatg ggcggcgctg cacgtctcac gctggggtaa tggtcgtggt
    19801 cgcttgctgt ttacctggat tgtcctgctc ggtgctgccg ttgccgccct gtttgccaat
    19861 gatggcgcgg cgcttatttt gacgccgatt gtcatcgcca tgctgctggc tttaggattc
    19921 agtaaaggca cgacgctggc gttcgtgatg gcggccggat tcattgccga taccgccagc
    19981 ctgccgctta ttgtctccaa cctggtgaat atcgtttccg ctgatttctt tggcctcggc
    20041 tttcgcgaat acgcctcggt gatggtgccg gtggatatcg ccgcgattgt tgccacgctg
    20101 gtgatgctac atctctattt tcgcaaagat attccgcaga actacgatat ggcgctgctg
    20161 aaatctcccg cagaagcgat caaagatcct gctacgttca aaactggctg ggttgtttta
    20221 ctgcttctgc tggtgggatt tttcgtcctg gaaccgctcg gcattccggt gagcgccatt
    20281 gcagctgtgg gcgcgctgat attatttgtc gtcgctaaac gcggtcatgc gattaatacg
    20341 ggtaaagtcc tgcgcggtgc cccctggcag attgtcatct tctcgctcgg catgtatctg
    20401 gtggtttatg gcctgcgcaa tgccggatta acggaatatc tttctggcgt actcaacgtg
    20461 ctggcggata acggcctgtg ggccgcgacg ctcggcaccg gattcctcac cgccttcctc
    20521 tcttctatta tgaacaatat gccgacggta ctggttggcg cgttgtccat tgatggcagc
    20581 acggcatctg gcgttatcaa agaagcgatg gtttatgcca atgtgattgg ctgcgatttg
    20641 ggaccgaaaa ttaccccaat tggtagcctg gctacgctac tctggctgca cgtactttcg
    20701 cagaagaata tgactatcag ctggggatat tacttccgta cagggattat catgaccctg
    20761 cctgtgctgt ttgtgacgct ggctgcgctg gcgctacgtc tctctttcac tttgtaatga
    20821 gatactgata tgagcaacat taccatttat cacaacccgg cctgcggcac gtcgcgtaat
    20881 acgctggaga tgatccgcaa cagcggcaca gaaccgacca ttatccatta tctggaaact
    20941 ccgccaacgc gcgatgaact ggtcaaactc attgccgata tggggatttc cgtacgcgcg
    21001 ctgctgcgta aaaacgtcga accgtatgag gagctgggcc ttgcggaaga taaatttact
    21061 gacgatcggt taatcgactt tatgcttcag cacccgattc tgattaatcg cccgattgtg
    21121 gtgacgccgc tgggaactcg cctgtgccgc ccttcagaag tggtgctgga aattctgcca
    21181 gatgcgcaaa aaggcgcatt ctccaaggaa gatggcgaga aagtggttga tgaagcgggt
    21241 aagcgcctga aataaagcgg cgatatccac tgccgatagt tggtaaagtg cgctttgttt
    21301 atgtcggatg cgacgctaac gcgtcttatc cgacctacaa aaccgtgcaa attcaatata
    21361 tggcatgaaa tatgtagagc gtagcgcatc aggcaatttt gctatttacg cagcactcct
    21421 cgcgtaagaa ttcaattatc gcttccagca ttgcgtattg cgaaacacac atcagcgtcc
    21481 gactctcacg gttttgtttg accagtccta ccgataccag tgctgatata tggtggctga
    21541 gtgtggagcc aggtatccca agctgctttt gcaactcacc gaccggcaac ccttgttccc
    21601 cggctttgac aagatgttta aatatgaaca aacgggtcgg gtgacctagc tctttcagcg
    21661 ctttagcgac ttcttccaac tgcatatgac acccctctgt ctggttgata tttcgataat
    21721 accagaaata tcttgatctg aatttcattt cgattattct ggaaatatag atttatccaa
    21781 tgaggttacg tatgagtagt tggcttgcga tgctgcaaga tgccgcagag atgtttgtgt
    21841 ttctcgccgt cgagctttcg ttgctgttta tagtgattag tgccggtgtc agcctgataa
    21901 gacaaaaggt gccagaccat aaaatccagc agatgatggg ggccagaaaa gggagaggct
    21961 atctcctggc tgctctgttg ggagccgtta ccccgttctg tagttgctcg acaataccca
    22021 tgttacgtgg attgttgtca gcgaaagccg ggt
//
LOCUS       DAJTZL010000058        21995 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.058, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000058 DAJTZL010000000
VERSION     DAJTZL010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21995
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.058"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..986)
                     /gene="cmtA"
                     /locus_tag="OO544_001912"
     CDS             complement(<1..986)
                     /gene="cmtA"
                     /locus_tag="OO544_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HCR6767521.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYVDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     LVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWVFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVATLVSFA"
     gene            complement(1014..1457)
                     /gene="cmtB"
                     /locus_tag="OO544_001913"
     CDS             complement(1014..1457)
                     /gene="cmtB"
                     /locus_tag="OO544_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HCR6767522.1"
                     /translation="MRLSDYFPESSISVIHSAKDWQEAIDFSMVSLLDKNYISENYIQ
                     AIKDSTINNGPYYILAPGVAMPHARPECGALKTGMSLTLLEQGVYFPGNDEPIKLLIG
                     LSAADADSHIGAIQALSELLCEEEILEQLLTAASEKQLADIISRG"
     gene            complement(1771..3762)
                     /gene="tkt"
                     /locus_tag="OO544_001914"
     CDS             complement(1771..3762)
                     /gene="tkt"
                     /locus_tag="OO544_001914"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCR6767523.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIIGMTTFGESAPAELLFEEFGFTVDNVVAKAKELL"
     gene            4040..4798
                     /gene="loiP"
                     /locus_tag="OO544_001915"
                     /gene_synonym="yggG"
     CDS             4040..4798
                     /gene="loiP"
                     /locus_tag="OO544_001915"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HCR6767524.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGIK"
     gene            complement(4941..5861)
                     /gene="speB"
                     /locus_tag="OO544_001916"
     CDS             complement(4941..5861)
                     /gene="speB"
                     /locus_tag="OO544_001916"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HCR6767525.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLTFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(5999..7975)
                     /gene="speA"
                     /locus_tag="OO544_001917"
     CDS             complement(5999..7975)
                     /gene="speA"
                     /locus_tag="OO544_001917"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HCR6767526.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(7984..8115)
                     /gene="yqgB"
                     /locus_tag="OO544_001918"
     CDS             complement(7984..8115)
                     /gene="yqgB"
                     /locus_tag="OO544_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HCR6767527.1"
                     /translation="MKKKPVAQLERQHSLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            8251..8466
                     /locus_tag="OO544_001919"
     CDS             8251..8466
                     /locus_tag="OO544_001919"
                     /inference="COORDINATES: protein motif:HMM:NF037550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6767528.1"
                     /translation="MGITSAGMQSRDAECGERIFTRTVRQVKQQTTVHYFVSPPRPPV
                     KTNPQAKTLISTRLEVATRKKRRVLFI"
     gene            8770..9924
                     /gene="metK"
                     /locus_tag="OO544_001920"
     CDS             8770..9924
                     /gene="metK"
                     /locus_tag="OO544_001920"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HCR6767529.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            10360..11754
                     /gene="galP"
                     /locus_tag="OO544_001921"
     CDS             10360..11754
                     /gene="galP"
                     /locus_tag="OO544_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HCR6767530.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            11831..12328
                     /locus_tag="OO544_001922"
     CDS             11831..12328
                     /locus_tag="OO544_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HCR6767531.1"
                     /translation="MKTSRLPIAIQQAVMRGLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            12423..13130
                     /gene="endA"
                     /locus_tag="OO544_001923"
     CDS             12423..13130
                     /gene="endA"
                     /locus_tag="OO544_001923"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HCR6767532.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMYNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            13210..13941
                     /gene="rsmE"
                     /locus_tag="OO544_001924"
     CDS             13210..13941
                     /gene="rsmE"
                     /locus_tag="OO544_001924"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014226833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HCR6767533.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGKIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            13954..14904
                     /gene="gshB"
                     /locus_tag="OO544_001925"
     CDS             13954..14904
                     /gene="gshB"
                     /locus_tag="OO544_001925"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HCR6767534.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTENGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            15013..15576
                     /locus_tag="OO544_001926"
     CDS             15013..15576
                     /locus_tag="OO544_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HCR6767535.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            15576..15992
                     /gene="ruvX"
                     /locus_tag="OO544_001927"
     CDS             15576..15992
                     /gene="ruvX"
                     /locus_tag="OO544_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HCR6767536.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(16167..17147)
                     /locus_tag="OO544_001928"
     CDS             complement(16167..17147)
                     /locus_tag="OO544_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HCR6767537.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFEAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRHGISLALRLLPSHCPQ
                     LEQLGAPTVLPELLKSENGLILVTGATGSSKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YIYASQRCLIQKREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSFQQRVGEGRL"
     gene            17165..17869
                     /gene="yggS"
                     /locus_tag="OO544_001929"
     CDS             17165..17869
                     /gene="yggS"
                     /locus_tag="OO544_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HCR6767538.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            17887..18453
                     /gene="yggT"
                     /locus_tag="OO544_001930"
     CDS             17887..18453
                     /gene="yggT"
                     /locus_tag="OO544_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HCR6767539.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            18450..18740
                     /locus_tag="OO544_001931"
     CDS             18450..18740
                     /locus_tag="OO544_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggU family protein"
                     /protein_id="HCR6767540.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQGNSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            18748..19341
                     /locus_tag="OO544_001932"
     CDS             18748..19341
                     /locus_tag="OO544_001932"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HCR6767541.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARYAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            19334..20470
                     /gene="hemW"
                     /locus_tag="OO544_001933"
     CDS             19334..20470
                     /gene="hemW"
                     /locus_tag="OO544_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HCR6767542.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            20781..21767
                     /locus_tag="OO544_001934"
                     /pseudo
     CDS             20781..21767
                     /locus_tag="OO544_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            21812..>21995
                     /locus_tag="OO544_001935"
     CDS             21812..>21995
                     /locus_tag="OO544_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HCR6767543.1"
                     /translation="MIFNRLKLAVDRVIAAFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLG"
BASE COUNT         5442 a         5715 c         5596 g         5242 t
ORIGIN      
        1 gcaaaggaca ccagggtagc taccgtaaca ccggcaattg tcgccaggaa cgagcctttc
       61 ggcgtcagtg ccaggtaagc aaagatagac cccggacttg ggccagccac cagaccaccg
      121 tccagtaagt taaacaccca ggtgccagac ataccgcccg caatcatggc aataatggtc
      181 agcggcttca tcagcacata cgggaaatac agttcgtgga tcccaccgag gaagtgaata
      241 atcatcgccc cgggcgcaga acgtttactc atccctttac caaacaaggt aaacgccagc
      301 agcagcccta agccaggccc aggattggac gccaccataa agaagatgga tttaccgtta
      361 accgaagcct gttgcattcc cagcggataa tagacgccct gatcgatcgc attattgagg
      421 aaaagcactt tcgccggttc attgatgact gacagcagag gcagataacc cgcatgtacc
      481 agcgcctcaa tacactcttt gacgagagta ttggcaatta acaccgccgg gccgataact
      541 tcaaaaccca gcagacaaag gagcatcccc gcgataccta atgagaagtt attgataacc
      601 atctcaaaac cggcaggtat gcgtttttcc agtgccttat caacatattt tatgaccagc
      661 ccaccgagcg gccccataat cattgagcca aggaacatcg ggatctctgc gccgacgatc
      721 acaccaatag tacctattcc gcccatgacc gcgccgcgtt taccgccgac cagatgacca
      781 ccggtagaac caatcatcac gggcaataaa taggtaatca tcgggccgac aattttggcg
      841 aaatgttcgt taggcagcca accggtggga ataaataacg cagtaataaa accccaggca
      901 ataaaagcac caatattggg gatgaccatt gccgtcaaaa agcccccaaa agcctggacc
      961 tttgcacgag cagacttgtt ttccataata ttatcctgta gaggagagag taattatccg
     1021 cggctgatta tgtccgctaa ttgtttttct gatgctgctg ttaagagttg ttcgagtatt
     1081 tcttcttcgc acagtaattc acttaacgcc tgaatagcgc caatgtgcga atcggcatcg
     1141 gcagcagaaa ggccgatgag taattttatt ggttcgtcat tccccggaaa ataaacacct
     1201 tgttcaagta atgtcaaaga catcccggtt ttaagcgccc cacattccgg tcgcgcatga
     1261 ggcattgcca cgcctggtgc gagaatataa taagggccat tgttaatggt ggaatcttta
     1321 attgcctgaa tgtaattctc gctgatatag tttttatcca gcaatgatac catcgagaaa
     1381 tcgatagctt cctgccaatc ttttgctgaa tgtataactg agattgatga ttctggaaaa
     1441 tagtcactaa gccgcataaa ttatccttat tttattttta gggtaccgtc cggcggtaaa
     1501 aataaaatac tcgtgcggtt taatgaaagc aattgagcag ggaaaccggt gttttgaaaa
     1561 cagtgaatta ttaatttctt atataacatt gagttataga tataacaaca aatgtcaata
     1621 cgcatatcgt gatgcatatt acataattga tatttattgc atataaatat ttgtgatcta
     1681 caacacgctt tatctattgc ttgtccgcaa acggacatat caaggtaata aaaaaggtcg
     1741 ccgaagcgac ctttttaccc gaaatgctaa ttacagcagt tcttttgctt tcgcaacgac
     1801 gttgtcgacg gtgaagccga actcttcgaa cagcaactct gccggagctg actcaccgaa
     1861 ggtggtcata ccgatgatag cgccattcag gccaacatac ttgtaccagt agtcagcaat
     1921 acccgcttct accgcaacgc gtgcagtaac cgctttcggc agtacggatt cacggtaagc
     1981 cgcatcctgc ttgtcgaatg catcggtaga cggcatggaa accacgcgcg ccttcacgcc
     2041 ttcggcagtc agtttttcgt aggcggcaac agccagttca acttccgaac cggtcgcgat
     2101 gaagatcagt tccggctgac cagcacagtc tttcagcaca taaccaccgc gcgcgatgtt
     2161 tgccagttgc tcttcagtac gttcctgctg cgccagattc tgacgggaga ggataagcgc
     2221 ggtcggaccg tcctgacgct caacaccgta tttccacgcg accgcggatt caacctggtc
     2281 acacggacgc catgtagaca tgttcggggt cacgcgcaga gaagcgacct gctcaaccgg
     2341 ctggtgagtc gggccgtctt cgcccagacc gatggagtcg tgggtgtaaa ccatcacctg
     2401 acgctgtttc atcagcgcag ccatacgtac agcgttacgt gcgtattcga caaacatcag
     2461 gaaggtggag gtgtacggca ggaagccacc gtgcagggag ataccgttag caatcgcggt
     2521 cataccgaac tcgcgaacac cgtagtggat gtagttaccc gcagcatctt cgttgattgc
     2581 tttagaacca gaccacaggg tcaggttaga cggtgccagg tcagcggagc cgccgaggaa
     2641 ttccggcaac agcgggccga acgcttcaat agcattctga gacgctttac ggctggcgat
     2701 tttcgccgga ttagcctgca gtttagcgat aaactctttc gctttggcgt cgaagtcaga
     2761 cggcatttcg cctttcatac ggcgggtaaa ttcagccgct tcctgcggat aagctttcgc
     2821 gtaagccgca aacttctcat tccatgcaga ttctttcgcc tggcctgctt ctttcgcatc
     2881 ccactgcgca tagatttcag acgggatttc gaacggtgcg tatttccagc ccagctgttc
     2941 gcgggtcaga gcgatttctg catcacccag cggcgcaccg tgggagtcgt gagtaccggc
     3001 tttgttcggg gaaccgaaac cgatgatggt tttgcacatc agcagggacg gtttgtcagt
     3061 cactgcgcgc gcttcttcta ctgcgcgttt gatggatgct gcgtcatgac cgtcgatgtc
     3121 gcgaataacg tgccagccgt aagcttcgaa acgcattgcg gtgtcgtcgg tgaaccagcc
     3181 ttcaacgtga ccatcaatag agataccgtt gtcatcgtag aacgcaatca gtttacccag
     3241 cttcagcgta cccgccagag agcaaacttc gtgggagatg ccttccatca tgcagccgtc
     3301 gcccatgaag gcgtaggtgt agtggtcgac aatgtcgtgg cccggacggt taaactgcgc
     3361 cgccagcgtt ttttctgcaa tcgccatacc gactgcgttg gcaataccct gacccagcgg
     3421 accggtggtg gtttccacac cagcggtata acctacttcc gggtggcccg gagttttaga
     3481 gtgcagctga cggaagtttt tcagttcttc catcggcaga tcgtaaccgg tgaggtgcag
     3541 caggctgtag atcagcatgg agccgtggcc gttggacagc acgaagcggt cacggtcagc
     3601 ccaggacgga ttctgcgggt tgtgtttcag gaaatcacgc cacaggactt cggcaatgtc
     3661 agccataccc ataggggcac ccgggtgacc ggatttggct ttctgtactg cgtccatgct
     3721 cagcgcacga atagcattgg caagctcttt acgtgaggac attttgactc cagatcggat
     3781 gatgaagggc acgcccttaa cgacttgacg acagcgcgtt ttgggctacg ccggaaaatt
     3841 tgccaacaat ttaccgcaag ccgcgcgtca tgtacatgga acatcctttt gccgcttcag
     3901 aaatctctgg atcatgctcg catgttgcgc aatctactcg cccgtccgct gcgcttttcc
     3961 ttatactgag actgagcgtc gattcacctg caaacggcgc atttttagaa taatcctgac
     4021 cttgtgcgga agagaaaaca tgaaaattcg cgccttattg gtagcaatga gcgtggcaac
     4081 ggtactgact ggttgccaga atatggactc caacggactg ctctcatcag gagcggaagc
     4141 ttttcaggct tacagtttga gtgatgcgca ggtgaaaacc ctgagcgatc aggcatgtca
     4201 ggagatggac agcaaggcga cgattgcgcc agccaatagc gaatacgcta aacgtctgac
     4261 aactattgcc aatgcgctag gcaacaatat caacggtcag ccggtgaatt acaaagtgta
     4321 tatggcgaag gatgtgaacg cctttgcaat ggctaacggc tgtatccgcg tctatagcgg
     4381 gctgatggat atgatgacag ataacgaagt cgaagcggtg atcggtcacg agatggggca
     4441 cgtggcgtta ggccatgtga agaaagggat gcaggtggcg cttggtacaa atgccgtgcg
     4501 agtagctgcg gcctctgcgg gcgggattgt cggcagttta tctcaatcac aacttggtga
     4561 tctgggcgag aaattagtca attcgcaatt ctcccagcgc caggaagcag aagccgatga
     4621 ttattcttac gatcttctgc gccaacgcgg catcagcccg gcaggtcttg ccaccagctt
     4681 tgaaaaactg gcgaaactgg aagaaggccg ccaaagctca atgtttgacg accatccggc
     4741 atccgccgaa cgcgcccagc atattcgcga tcgcatgagc gcggatggga ttaagtaaag
     4801 cctggtggtg tcgaacgtag gtcagataag gcgttcacgc cgcatctgac atcaatggca
     4861 catttgttac cctgtgcgca ttgccggatg cgatgctgac tgcatcttat ccggcctacg
     4921 ggtggcatcg catctggtgc ttactcgccc tttttcgccg cttggatata cagcatctcc
     4981 agcgccagcg tcgccgcagc cagagcggtg atttccgact gatcgtatgc cggagccact
     5041 tccactacgt ccatcccaac aatgttgaga tctttcaggc cgcgtaccag tttaatagcg
     5101 cgatcggagg tcaggccgcc aatcactggc gtaccggtgc ctggtgcaaa agcaggatcc
     5161 aggcagtcga tatcaaaagt caggtaaacc ggcatatcac ccacaatctg tttcacttgg
     5221 gcgataacgt catccacgct gcgatcgttc acctggcagg cgtccagcac ggtaaagccg
     5281 ttgtctttat caaactcggt acgaatacca atctgcacgg aatgattcgg gtcgatcaga
     5341 ccttctttcg gcgcggtata gaacatagtg ccgtggtcaa attcacaacc gttcgcatag
     5401 gtatcggtgt gggcgtcaaa gtgtaccagc gccattttgc cgaaatgctt cgcatgagca
     5461 cgcagcagcg gcagcgtaac aaagtggtca ccaccgaagg tgagcatacg cttaccggca
     5521 gccagcagct tctcggcgtg cgcctgcagc ttttcgctca tctcacgggc atcgccaaag
     5581 gcatatacca gatcgccgca gtccacgacg ttcagacgct cacgcatgtc gaaattccac
     5641 gggaagcggt tgtgttccca ggccagattc gtcgaaacct gacggatcgc tgccggaccg
     5701 tggcgaccac ccgcacgacc agaagtggcc atatcgaacg gcacgccagt aatcacccag
     5761 tctgcatcgc tgtcatacgg ctggaagttc atcggcaggc gtaaaaaacc aaaggcattg
     5821 gaaaccagtg agttatcgta ttgatgacct aaggtgctca tggatggact cctattacaa
     5881 agtcgatata aaaaaaccct tccgcgtcgt taggcccgac gaggaagggt tggatttgtc
     5941 acaataaatt gtggcggatt atcaccgcta aattaagcgg attcaagtaa cacaggactt
     6001 actcatcttc aagataagta taaccgtaca aacctgcctc gaactcttca aggaactgtt
     6061 gttgcagttc agcatcaaga tcggttttct tcacttgatc gcggaactgg gttaacagcg
     6121 ttttcggatc gagctgtaca tattgcagca tgtccgccac ggtatcgcct tcgtcagaca
     6181 gttctacttc tacgctaccg tcagggaaca cgaacacgtc aaccgcttcg gtatcaccga
     6241 acaggttgtg catattgcca aggatctcct gatatgcgcc gaccataaag aaaccgagca
     6301 tcggcggatt ctctggatcg tactccggca ttggcattgt cgtggcaata ccatcaccat
     6361 caatatagtg gtcgatagca ccgtcagagt cacaggtaat atccagcagc acagcgcgac
     6421 gttccggcac ttgatccagc ccttccagcg gcagaaccgg gaacaactgg tcgatccccc
     6481 atgcgtccgg cattgactgg aacagcgaga aattgacgta cattttgtcc gccatacgtt
     6541 cctgcagttc gtcgattatc ggacgatgcg cacggttttg cggatccagt tgcttctgca
     6601 cttcatggca catgctcaga tagagttgtt ctgcccatgc acgttcttgc aggctaaagg
     6661 tgccggaaga gtagccgata tgaatgtcgt gcagatccat ctggctgtcg tgtaaccatt
     6721 cacgcaaaga acggcgagtt cccggttcgt gcatttcctg ccaggtttcc cacatgcttt
     6781 gcagcgcgcg cggcgcatct tctgcgggcg cggtcggcac cgtatattcg ttacgttcca
     6841 cgccgatgat attcgacacc agcacggtat ggtgcgcagt caccgcacgg cccgattcgg
     6901 tgattaccgt cggatgcggc agaccatttt cttcacacgc atcgccaatc gcccagatga
     6961 tgttgttggc gtattcattg aggccatagt ttacggagca gtcagactgc gaacgggtgc
     7021 cttcatagtc cacgcccaga ccgccgccga cgtcgaagca ctgaatattg acgcccagct
     7081 tgtgcagttc cacatagaaa cgcgcggatt cacgaacgcc cgtggcgata tcgcgaatat
     7141 tcgccatctg cgaaccgagg tggaagtgca gtagttgcag gctgtcgaga cgcccggctt
     7201 cacgcagggt ttcaaccagt tgcagtacct gagtcgcagc caggccgaac ttcgattttt
     7261 ccccgccgga ggactgccat ttacccgagc cctgcgaagc cagacgtgca cgcacgccca
     7321 gacgaggaac gacattcaga cgttctgctt catccagcac aatggcgatt tctgacatct
     7381 tctcaatgac cagatagacc ttgtgcccca tcttctcgcc aattaatgcc aggcggatat
     7441 attcgcggtc tttataaccg ttgcagacga tgacgctacg ggtcatgcca gcatgtgcca
     7501 gtactgccat caactcggct ttggaaccgg cttccagacc cagcggttcg cccgaatgaa
     7561 tcagggactc aatcacgcgg cggtgctggt taactttgat cggataaaca aggaagtaat
     7621 cgccgttata gccgtaggat tccctcgcac gtttgaacgc ggcgttaatg gaacgcaaac
     7681 ggtgctgcag gatctgtggg aaacagaaca gtgcaggcag acgctgaccc tgtgcttcac
     7741 gagttttcac taactgcgcg agatcgacgc gagcttccgg gacgtccggg tccgggcaca
     7801 cgctgatgtg gcccagctcg ttaacgtcat agtagttatt gccccaccag gcaatattgt
     7861 aagtacgcag catcttgctg gcttcctggg agctcattgc aacctcctgc atggagcgta
     7921 gtacaccgtg ttcgcccgct gacgaaggca aacccataga catgtcgtca gacatagcga
     7981 acctcaaatt attttattaa gtgtaaaaca gttaacgact atcgcagcct ggaactgcga
     8041 taacaaccca taagcacatg gattttccag cagtgaatgc tgacgctcca actgcgcaac
     8101 cggtttcttt ttcataacat tattaagcac ataaccgaac gtaagtgtga cagttcggcg
     8161 aaaccacgag aaaactcttg tttttacaag agcgcccttg ttcagtcctc agtaactgta
     8221 accagctctt gaatcctgag aagcgccgag atgggtataa catcggcagg tatgcaaagc
     8281 agagatgcag agtgcgggga acgaatcttc accagaacgg tgagacaggt taagcagcag
     8341 acaacggttc attatttcgt atcacctcca cggccgcctg ttaagacgaa cccacaagcc
     8401 aaaactctga tttcaacccg gctggaagtg gcaacacgaa agaaacgtcg tgtgcttttt
     8461 atttaagccg cgcgccgcgt tttatacccc acaatggcag aaaattgcaa aagataaata
     8521 cgcagaatgc cggcattgtc aggaaaaatt tccagccacg tttttaacag aatgagacac
     8581 gattcaaaaa aaagtggaaa tcgggtgaag aattgaccta aaatagccat ccagatgtta
     8641 atccatccat accgattaac actcagactg ccagtgtttt taacctgcag agtcgtggta
     8701 ggatccgcta ccacagaaaa tccacacaac agtttgagct aaccaaattc tctttaggtg
     8761 atattaaata tggcaaaaca cctttttacg tccgagtccg tctctgaagg gcatcctgac
     8821 aaaattgctg accaaatctc tgatgccgtt ttagacgcga tcctcgaaca ggatccgaaa
     8881 gcacgcgttg cttgcgaaac ctacgtaaaa accggcatgg ttttagttgg cggcgaaatc
     8941 accaccagcg cctgggtaga catcgaagag atcacccgta acaccgttcg cgaaattggc
     9001 tatgtgcatt ccgacatggg ctttgacgct aactcctgtg cggttctgag cgctatcggc
     9061 aaacagtctc ctgacatcaa ccagggcgtt gaccgtgccg atccgctgga acagggcgcg
     9121 ggtgaccagg gtctgatgtt tggctacgca actaatgaaa ccgacgtgct gatgccagca
     9181 cctatcacct atgcacaccg tctggtacag cgtcaggctg aagtgcgtaa aaacggcact
     9241 ctgccgtggc tgcgcccgga cgcgaaaagc caggtgactt tccagtatga cgacggcaaa
     9301 atcgttggta tcgatgctgt cgtgctttcc actcagcact ctgaagagat cgaccagaaa
     9361 tcgctgcaag aagcggtaat ggaagagatc atcaagccga ttctgcccgc tgaatggctg
     9421 acttctgcca ccaaattctt catcaacccg accggtcgtt ttgttatcgg tggcccgatg
     9481 ggtgactgcg gtctgactgg tcgtaaaatt atcgttgata cctacggcgg catggcacgt
     9541 cacggtggcg gagcattctc tggtaaagat ccatcaaaag tggaccgttc cgcagcctac
     9601 gcagcacgtt atgtcgcgaa aaacatcgtt gctgctggcc tggccgatcg ttgtgaaatt
     9661 caggtttcct acgcaatcgg cgtggctgaa ccgacctcca ttatggtaga aactttcggt
     9721 actgagaaag tgccttctga acaactgact ctgctggtac gtgagttctt cgacctgcgc
     9781 ccatacggtc tgattcagat gctggatctg ctgcacccga tctacaaaga aaccgcagca
     9841 tacggtcact ttggtcgtga acatttcccg tgggaaaaaa ccgacaaagc gcagctgctg
     9901 cgcgatgctg ccggtctgaa gtaatctttc ttcacctgct ttcaaaggcc agcctcgcgc
     9961 tggccttttt cttttacctc accgcggtgg ataggcattc acgccgcatc cggcaaaaag
    10021 ctgcccgcac aataacatca ttcttcctca acacgtttta tcgcaaatta tcatcactac
    10081 tgaaaccgat tacaccaata acaccagcca aatatttgta atattttcat attattattc
    10141 agttttcaca gttgttacat ttcttttcag taaagtctta attgcagata acagcgttta
    10201 atctatgatg atataactca attattttca tgcacttaaa ccataactaa gataaatgtt
    10261 agtgtaagcg attacactga tgtgatttgc ttcacatctt tttacgtcgt actcacctat
    10321 cttaattcac aataaaaaat aaccatattg gagggcatca tgcctgacgc taaaaaacag
    10381 gggcggtcaa acaaggcaat gacgtttttc gtctgcttcc ttgccgctct ggcgggatta
    10441 ctctttggcc tggatatcgg tgtaattgct ggcgcactgc cgtttattgc agatgaattc
    10501 cagattactt cgcacacgca agaatgggtc gtaagctcca tgatgttcgg tgcggcagtc
    10561 ggtgcggtgg gcagcggctg gctctccttt aaactcgggc gcaaaaagag cctgatgatc
    10621 ggcgcaattt tgtttgttgc cggttcgctg ttctctgcgg ctgcgccaaa cgttgaagta
    10681 ctgattcttt cccgcgttct actggggctg gcggtgggtg tggcctctta taccgcaccg
    10741 ctgtacctct ctgaaattgc gccggaaaaa attcgtggca gtatgatctc gatgtatcag
    10801 ttgatgatca ctatcgggat cctcggtgct tatctttctg ataccgcctt cagctacacc
    10861 ggtgcatggc gctggatgct gggtgtgatt atcatcccgg caattttgct gctgattggt
    10921 gtcttcttcc tgccagacag cccacgttgg tttgccgcca aacgccgttt tgttgatgcc
    10981 gaacgcgtgc tgctacgcct gcgtgacacc agcgcggaag cgaaacgcga actggatgaa
    11041 atccgtgaaa gtttgcaggt taaacagagt ggctgggcgc tgtttaaaga gaacagcaac
    11101 ttccgccgcg cggtgttcct tggcgtactg ttgcaggtaa tgcagcaatt caccgggatg
    11161 aacgtcatca tgtattacgc gccgaaaatc ttcgaactgg cgggttatac caacaccaca
    11221 gagcaaatgt gggggacagt gattgtcggc ctgaccaacg tacttgccac ctttatcgca
    11281 atcggccttg ttgaccgctg gggacgaaaa ccaacgctaa cgctgggctt cctggtgatg
    11341 gctgctggca tgggcgtact cggtacaatg atgcatatcg gtatccactc tccgtcggcg
    11401 cagtatttcg ccatcgccat gctgctgatg tttattgtcg gttttgccat gagtgccggt
    11461 ccgctgattt gggtactgtg ctccgaaatt cagccgctga aaggccgcga ttttggcatc
    11521 acctgctcca ctgccaccaa ctggattgcc aacatgatcg ttggcgcaac gttcctgacc
    11581 atgctcaaca cgctgggtaa cgccaacacc ttctgggtgt atgcggctct gaacgtactg
    11641 tttatcctgc tgacattgtg gctggtaccg gaaaccaaac acgtttcgct ggaacatatt
    11701 gaacgtaacc tgatgaaagg tcgtaaactg cgcgaaatcg gcgctcacga ttaatctcct
    11761 caggcttcct cccgtcgcgg aggaagccgc ctcttgcagt catcttttct tcgctctatc
    11821 ctctgccgct atgaaaacat cccgtctccc tatcgccatc caacaggccg ttatgcgcgg
    11881 cctgcgggaa aaactcgccc aggccaacct gaagctaggg cgtaactacc cggagccaaa
    11941 actctcttac acccagcgcg gaacctccgc cggaacagcc tggctggaaa gctatgaaat
    12001 ccgcctcaat cccgttttgc tgttggaaaa cagtgaagct tttattgaag aagtggtacc
    12061 gcacgaactg gcacatttgc tggtatggaa acatttcggc cgcgtagcgc cacatggcaa
    12121 agagtggaag tggatgatgg aaagcgtgct gggtgttccc gcccgtcgta cgcatcagtt
    12181 cgaactgcaa tccgtgcgtc gcaacacctt cccctaccgc tgcaagtgcc aggagcatca
    12241 gcttaccgta cgccgccata atcgcgtagt tcgtggcgag gccgtctatc gctgtgttca
    12301 ctgcggtgaa cagctggttg cgaaataacc atctgaacta tcaggaactt tcctgatctg
    12361 gctgattgca taccaaaaca actttcgcta cgttgctggc tcgttttaac acggagtaag
    12421 tgatgtaccg ttatttgtct attgctgcgg tggtactgag cgcagcattt tccggcccgg
    12481 cgttggccga aggtatcaat agtttttctc aggcgaaagc cgcggcggta aaagtccacg
    12541 ctgacgcgcc cggtacgttt tattgcggat gtaaaattaa ctggcagggc aaaaaaggcg
    12601 ttgttgatct gcaatcgtgc ggctatcagg tgcgcaaaaa tgaaaaccga gccagccgcg
    12661 tagagtggga acacgtcgtt cccgcctggc agttcggtca ccagcgccag tgctggcagg
    12721 acggtggacg taaaaactgc gctaaagatc cggtctatcg caagatggaa agcgatatgt
    12781 ataacctgca gccgtcagtc ggtgaggtga atggcgatcg cggcaacttt atgtacagcc
    12841 agtggaatgg cggcgaaggc cagtacggtc aatgcgccat gaaggtcgat ttcaaagaaa
    12901 aagctgccga accaccagcg cgtgcacgcg gtgccattgc gcgcacctac ttctatatgc
    12961 gcgaccaata caacctgaca ctctctcgcc agcaaacgca gctgttcaac gcatggaaca
    13021 agatgtatcc ggttaccgac tgggagtgcg agcgcgatga acgcatcgcg aaggtgcaag
    13081 gcaatcataa cccgtatgtg caacgcgctt gccaggcgcg aaagagctaa cctacactag
    13141 cgggattctt tttgttaacc cctaccccac gcgtacaacc gcgtggggag acgacgcgga
    13201 tttttaacta tgcgtatccc ccgcatttat catcctgaac cactgaccag ccattctcac
    13261 atcgcgcttt gcgaagatgc cgccaaccat atcgggcgcg tactgcgcat ggggccgggg
    13321 caggcgttgc aattgtttga cggtagcaac caggtctttg acgccgaaat taccagcgcc
    13381 agcaaaaaaa gcgtggaagt gaaggtgctg gaaggcaaga tcgacgatcg cgaatctccg
    13441 ctgcatattc acctcggtca ggtgatgtcg cgtggtgaaa aaatggaatt tacgatccag
    13501 aaatcgatcg aactcggtgt aagcctcatt acgccacttt tttctgagcg ctgcggcgtt
    13561 aaactggata gtgaacgtct gaacaagaag ctccagcagt ggcagaagat tgcaattgct
    13621 gcctgtgagc agtgtggtcg taaccgggtg ccggaaatcc gtccagcgat ggatctggaa
    13681 gcctggtgtg cagagcagga tgaaggactg aaactgaatc ttcacccgcg cgccagtaac
    13741 agcatcaata cgttgccgtt accggttgaa cgcgtccgcc tgctgattgg cccggaaggc
    13801 ggtttatcgg cagatgaaat tgccatgact gcccgctatc aatttactga tatcctgttg
    13861 ggacctcgcg ttttgcgtac agagacaact gcgctcaccg ccattaccgc gctacaagta
    13921 cgatttggcg atttgggcta acggagaaga ataatgatca agctcggcat cgtgatggac
    13981 cccatcgcaa acatcaacat caagaaagat tccagtttcg ctatgttgct ggaagcacag
    14041 cgtcgtggtt acgaacttca ctatatggag atgggcgatc tgtatctgat caatggcgaa
    14101 gcccgcgccc atacccgcac gctgaacgtg aagcagaact acgaagagtg gttttcgttc
    14161 gtcggtgaac aggatctgcc gctggccgat ctcgatgtga tcctgatgcg taaagacccg
    14221 ccgtttgata ccgagtttat ctacgcgacc tatattctgg aacgtgccga agagaaaggg
    14281 acgctgatcg ttaacaagcc gcagagcctg cgcgactgta acgagaaact gtttaccgcc
    14341 tggttctctg acttaacgcc agaaacgctg gttacgcgca ataaagcgca gctaaaagcg
    14401 ttctgggaga aacacagcga catcattctt aagccgctgg acggtatggg cggcgcgtcg
    14461 attttccgcg tgaaagaagg cgatccaaac ctcggcgtga ttgccgaaac cctgactgag
    14521 aatggcactc gctactgcat ggcacaaaat tacctgccag ccattaaaga tggcgacaaa
    14581 cgcgtgctgg tggtggatgg cgagccggta ccgtactgcc tggcgcgtat tccgcagggg
    14641 ggcgaaaccc gtggcaatct ggctgccggt ggtcgcggtg aacctcgtcc gctgacggaa
    14701 agtgactgga aaatcgcccg tcagatcggg ccgacgctga aagaaaaagg gctgattttt
    14761 gttggtctgg atatcatcgg cgaccgtctg actgaaatta acgtcaccag cccaacctgt
    14821 attcgtgaga ttgaagcaga gtttccggtg tcgatcaccg gaatgttaat ggatgccatc
    14881 gaagcacgtt tacagcagca gtaacccacc ttagcgagaa ggatctcgtt gagactctga
    14941 gtgacagcgc ccttctttcc acgcatactg ggcgctgttg cttttttgaa ccaggaaaca
    15001 gaacctctga caatgaattt acagcatcac tttcttattg ccatgcctgc tctccaggat
    15061 ccgattttcc gtcgttccgt ggtctacatt tgcgaacata ataccaatgg tgcaatgggg
    15121 atcatcgtca acaagccgct ggaaaatctc aaaattgaag ggattctgga aaagctgaag
    15181 atcacgccgg agccgcgtga tgaatcaatc cgtctggata aaccggttat gctcggcggt
    15241 ccgctggctg aagatcgcgg gtttattttg catactccgc cctccaattt tgcttccagc
    15301 attcgcattt cagacaacac agtgatgacc acttcccgcg atgtgctgga aacgctcggc
    15361 accgataaac aaccgtctga cgtattggtg gctctaggtt atgcctcctg ggaaaaaggt
    15421 caactggaac aagaaattct cgataacgcg tggctaacgg ccccggcgga tctgaatatt
    15481 ctgttcaaaa cgccgattgc cgaccgctgg cgcgaggcgg caaaactgat tggtgtggat
    15541 attctcacca tgcctggtgt ggcagggcac gcctgatgag tggaacctta ctcgccttcg
    15601 acttcggcac caaaagcatt ggcgtagcgg tcggccaacg cattaccggc actgctcgcc
    15661 ctttgcctgc aattaaagca caggacggta cgccggactg gaacctcatc gaacgtttac
    15721 tgaaagagtg gcagccggac gaaatcatcg tcggtttgcc gctgaatatg gacggcaccg
    15781 agcaaccatt gactgccaga gcgcgtaaat ttgccaaccg tattcatggt cgtttcggtg
    15841 ttgaagtaaa gctccatgac gaacgtctta gcaccgtgga agcccgttcc ggtctgtttg
    15901 aacagggcgg ctatcgggcg ctcaacaaag gcaaagtcga ctctgcctct gcggttatta
    15961 ttctcgaaag ctatttcgag cagggatatt aaggcgattt aaaagcctgg cggatagtcg
    16021 ttaccgccat cttgccggat gcggcgtgaa cgcctatccg gcctacatgt ggcagcgatt
    16081 gtaggtctga taagacgcgc aaacgtctca tcagacgttt attgcctttg taggcccgat
    16141 aagcttgcac atcgggcatg gcaacgtcac aaacgccctt cccccactcg ctgctgaaaa
    16201 ctctgctgaa acgttatcat ccccacctgc tgcccggttt gaataacatg cggtaactgg
    16261 tgggttttcc cttcgcgaat caaattcccc accgcgggag tgttaatcaa taattcaaac
    16321 agcgccacgc gtccttcctg tttatccact tccagctttt gtgacaacac ggcccgtaaa
    16381 ctacctgcca gttgattacg tacgggatct ttttcctgcg ccggaaatga atccaccagt
    16441 cgctcaactg cctgcgcggc accacgcgta tgtaatgttg ccagcaccaa atgcccggtt
    16501 tctgccgccg tcagtgccag acggattgtc tcgctgtcac gcagctctcc gagcaaaatc
    16561 acatcaggat cttcccgcaa tgcggcccgc aatcccgatg cgaacgtcat acagtgcaaa
    16621 ccaatttccc gcttctggat caaacatcgc tggctggcat agatatattc aacaggatct
    16681 tccagcgtca gaatatgcgc atcggcatgt tgattgagat agccaaccat cgccgccagc
    16741 gtggtagatt tgctgctccc cgtcgccccc gtcaccagaa tcaggccatt ctcactcttg
    16801 agtaattccg gcaataccgt tggtgcgcca agctgttcga gctgcgggca gtgcgaaggt
    16861 aacagccgta acgccagcga aatgccatgc cgttgcgcaa atgcgctgcc acgcaatcgc
    16921 tggttttccg ccagcgacac ggcaaaatcc agctggccat tttccagcaa tattgcccgc
    16981 tgatcgtcat ccagccactc ccgcagtagc tcttcgacgt ccggcgcctc aaacggcgca
    17041 gcttccatta gcccgcgaat gcgccatcgt gcgggccagg cgctgcacag gtgtagatcc
    17101 gagacgttat gctttacact aagggccaca atttcttcca tattcatact aagatcctcg
    17161 gaaaatgaac gatattgcgc ataacctggc acaggtccgg gacaaaatct cagcggctgc
    17221 aacgcgttgc ggccgttctc cagaagaaat tacgctgctt gcagtcagta aaacaaaacc
    17281 tgcgagcgcc atcgcagaag ccattgatgc cgggcagcgt caatttggtg aaaactacgt
    17341 tcaggaaggg gtagataaaa ttcgccactt tcaggaactg ggcgtaacag gattagaatg
    17401 gcattttatt ggcccgttgc agtctaataa aagccgcctg gtggcagagc atttcgactg
    17461 gtgtcatacc atcgaccgtt tgcgcattgc tacccgcctc aacgatcagc gcccggcaga
    17521 acttccccct cttaacgtac tgattcaaat taatattagt gatgaaaaca gtaagtccgg
    17581 gattcaactg gcagaactgg acgagctggc agcggcggtc gctgaactac cgcgtttacg
    17641 tctgcgcggg ctgatggcaa tccctgcgcc tgagtcagaa tatgtaaggc agtttgaagt
    17701 tgcacgccaa atggctgtag catttgccgg actgaaaacg cgctacccgc atatcgacac
    17761 gctctctctg ggaatgtcgg acgatatgga agccgccatt gcggcaggta gcacgatggt
    17821 tcgtatcggc actgcaattt ttggtgcgcg tgattactct aaaaaataag gaattaaagg
    17881 aacgccatga atacgttgac tttcctgctt tcaacggtca ttgagctgta taccatggtg
    17941 ctgttattac gcatctggat gcagtgggct cattgtgatt tttacaaccc cttctcacag
    18001 tttgtagtga aggtaacgca gccaattatc gggccactgc gccgcgttat tccggcaatg
    18061 ggccccattg acagcgcctc gctgctggtt gcctatattc tcagttttat caaagccatc
    18121 gtgctgttta aagtggtgac cttcctgcca atcatctgga ttgctggttt actgattctg
    18181 ctgaaaacca tcggcctgct gattttctgg gtcctgctgg tgatggcgat tatgagctgg
    18241 gtaagccagg gtcgtagtcc gattgaatac gtgctgattc agctggccga tccgctgctg
    18301 cgcccgattc gccgcctgct accggcaatg ggtgggattg atttctcgcc gatgatcctc
    18361 gttctgctgc tgtatgtcat caatatgggt gtcgcagaag tattacaggc aaccggaaac
    18421 atgctgctgc cggggctgtg gatggcgtta tgagtgccgt aacagttaat gatgacggtc
    18481 tggttttacg gctctatatt cagccgaaag ccagccgtga ttctattgtc ggtttacatg
    18541 gcgacgaagt taaagtcgcc attaccgcgc cgccggttga cggccagggc aacagtcatc
    18601 tggtgaagtt tctcggtaag caattccggg ttgccaaaag ccaggtggtg attgaaaaag
    18661 gcgaacttgg ccgccacaaa caaattaaaa tcattaatcc gcaacaaatc ccgccagaaa
    18721 tcgcggcgtt aattaattag gtatcctatg caaaaagttg tcctcgcaac cggcaatgcc
    18781 ggtaaagtgc gtgagctggc gtcgctgctt agcgacttcg gtcttgatat cgtggcccaa
    18841 acagacctcg gcgtagattc cgctgaagaa accggcctga cctttatcga aaacgcgatt
    18901 ctgaaagcgc gctatgcggc aaaagtgacc ggtttaccgg caattgccga cgactctggt
    18961 ctggcggtag atgcgctggg tggcgcgccg ggtatttact ccgcgcgtta ttccggtgaa
    19021 gacgcgaccg atcaaaagaa tctgcaaaaa ctgttggaaa cactgaaaga cgtaccggac
    19081 gaccaacgtc aggcgcgttt ccactgcgtg ctggtatatc tgcgtcacgc ggaagatccg
    19141 actccgctgg tatgccacgg cagctggccg ggcgtgatta ctcgcgaacc agcgggcact
    19201 ggtggctttg gttatgatcc aatcttcttc gtaccttccg aagggaaaac cgccgccgaa
    19261 ctgacccgcg aagaaaagag cgccatttcc caccgtggtc aggcgttgaa actgctgctg
    19321 gacgctttac gtaatggtta aattaccgcc gctgagtctc tacattcaca tcccgtggtg
    19381 cgtgcagaaa tgcccgtact gcgatttcaa ctctcacgcg ttgaaaggag aagtgccgca
    19441 cgacgattat gttcagcatc tgctttgcga tctggacaat gatgtggctt acgctcaggg
    19501 ccgtgaagta aagacaatct ttattggcgg tggtacgccg agcctgcttt ccggcccggc
    19561 gatgcaaacg ctgctggacg gcgtgcgtgc gcgtttgccg ctggcagcgg atgcagaaat
    19621 tactatggaa gcgaaccctg gaacggtaga agccgatcgc tttgtcgatt atcagcgtgc
    19681 tggtgtgaac cgcatctcta ttggtgtgca gagttttagc gaagaaaagc tgaaacgact
    19741 tgggcgtatt catggcccgc aagaagcgaa acgcgcggca aagctggcga gcgggctggg
    19801 gctgcgtagc tttaacctcg atttgatgca tggactaccg gatcaatcac tggaagaggc
    19861 gcttggcgat ctacgccagg ccattgaact gaatccgccg catctttcct ggtatcaact
    19921 gaccatcgaa cccaatacgc tgtttggttc gcgaccaccg gtgctgccgg acgatgacgc
    19981 gctgtgggat attttcgaac aggggcatca gttattaacc gcagcgggtt atcagcaata
    20041 tgaaacttcc gcttacgcca aacccggtta tcagtgccag cacaatctca actactggcg
    20101 atttggtgac tacatcggta ttggctgcgg cgcgcacggc aaagtcacct tcccggatgg
    20161 gcgcattctg cgtaccacta aaacgcgtca tcctcgtggt tttatgcaag gaaggtatct
    20221 ggaaagccag cgtgatgtcg aagccgcaga taagccgttt gagttcttta tgaatcgctt
    20281 ccgtctgctg gaggccgcgc cgcgcgtgga gtttattgcg tataccgggc tttgcgaaga
    20341 tgtgattcgc ccacagttag acgaggcgat tgcccagggt tatctcaccg aatgtgcgga
    20401 ttactggcag ataaccgagc acggtaaatt attcttaaac tcacttcttg aattattcct
    20461 cgctgagtaa tcattaatgc gcctgcgggc gcattaatca cttcttttcc ccccgttaat
    20521 taacttcggc aaaatatatt tcttactgaa taaacatcga tgatatttat tagatatatc
    20581 tagtgaatat ttactcatca tttattgatt ataaacatgt acaacctggc gcacaaattt
    20641 aatatttaat ttctgacttt atttcaagtt gtgataaaca tagacaagtt gtcagatagg
    20701 ttaaccaatt tgtcgcacaa agcactgata agcacaatta gatatcaacc aatttccatc
    20761 cttcaacagg gagttttttt atgaaaatga ggtcatttac ccgctcatta gtctgcgcat
    20821 ccttgctggc tctggttagc actggcgtaa acgccgcaga aaaagtgacg ttaaagttag
    20881 cacacaacct ggaaagaagt catgttgtac atcagagttt tgaagagctg gcgaaagagg
    20941 taaaacagct gtcagaaggt aatatggtga tccgtattta tcccagcagc cagatgggta
    21001 atgcgcgata aactatggaa ttgttgcaaa atggcgcact ggatatgacc aaaggttccg
    21061 ccagtgatct ggagtctttt gataatattt atgctatcta taatttgccg ttcttattta
    21121 aagatcaggc tcacttcaac aaagttgttt ttggtgaagt tggtaaagaa attatggact
    21181 caacaaaaga caaaggcttt tttgctttat cagcttatgt agcgggtaca cgcagtttct
    21241 atgcgaaaaa accgattact aagccagaag atcttaaagg gctgaaaatt cgtgttcagc
    21301 caagtccgac caccatcaaa atgattgaac tgatgggtgg ttcacccact ccaatttcat
    21361 ttggcgaagt ttatactgcg atgcagcaag gtgtagtaga tggcgcagaa aataacgtgc
    21421 cttcctgggt gcagactcga catattgaaa ttgccaaagt tttttctgaa gatgaacacg
    21481 cctcaattcc tgactttttg gttatctcca ccaaaacatg gaataaatta accccagaac
    21541 agaaacaaat cctcgaaaca gcagcgaaga aatccgaagc ttatcaacaa aaattgtggg
    21601 aaaaaattga cgctgacaca cgtgctcagg cgaaagctat ggggggtgaa attgtaaaag
    21661 tggataaagc accattccgt gccgcagttc agcctctgtt tgacgacttc aaaaaagatc
    21721 caaaacaagc tgctttgtta gagaagttcg acaacgctgc tcaataatat tccgggccag
    21781 tctctggccc tgattttcca agatgcttac aatgatattt aaccgcctga aacttgcggt
    21841 agaccgcgtc attgccgcgt tttctgtcgc cgtcatgctt gctctggttg tatgcgtagt
    21901 ctggcaagta tttagccgct acgtacttaa ccagccaagt acattaaccg atgagctggc
    21961 acgatttttg atgatctggg tagggttatt aggtg
//
LOCUS       DAJTZL010000059        21745 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.059, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000059 DAJTZL010000000
VERSION     DAJTZL010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21745
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.059"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..608)
                     /locus_tag="OO544_001936"
     CDS             complement(<1..608)
                     /locus_tag="OO544_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HCR6767544.1"
                     /translation="MIIERCRELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLARAGEKTPPDALEKLTD
                     PLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSSIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRALTGEEPRVAMLS"
     gene            complement(605..1408)
                     /gene="eutT"
                     /locus_tag="OO544_001937"
     CDS             complement(605..1408)
                     /gene="eutT"
                     /locus_tag="OO544_001937"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HCR6767545.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLGC
                     QAIVGLSDEDLHRLSHQPLRYLDHDHLVPEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITRSFEVLRPDILQALNRLSSTVYVMMILSVTKPPLTVKQIQQRLGETQ"
     gene            complement(1405..2106)
                     /gene="eutQ"
                     /locus_tag="OO544_001938"
     CDS             complement(1405..2106)
                     /gene="eutQ"
                     /locus_tag="OO544_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HCR6767546.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEARKVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGAMQPSFKSVTGKGGIKVIDGSSVKFGRFDGAQPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGELHVRHEGETMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            complement(2081..2560)
                     /gene="eutP"
                     /locus_tag="OO544_001939"
     CDS             complement(2081..2560)
                     /gene="eutP"
                     /locus_tag="OO544_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HCR6767547.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(2573..2908)
                     /gene="eutS"
                     /locus_tag="OO544_001940"
     CDS             complement(2573..2908)
                     /gene="eutS"
                     /locus_tag="OO544_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HCR6767548.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGVIGIM
                     TLTPGETAMIVGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            complement(3201..5480)
                     /gene="maeB"
                     /locus_tag="OO544_001941"
     CDS             complement(3201..5480)
                     /gene="maeB"
                     /locus_tag="OO544_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HCR6767549.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRERAPELMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            5769..6719
                     /gene="tal"
                     /locus_tag="OO544_001942"
     CDS             5769..6719
                     /gene="tal"
                     /locus_tag="OO544_001942"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCR6767550.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQYAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            6739..8742
                     /gene="tkt"
                     /locus_tag="OO544_001943"
     CDS             6739..8742
                     /gene="tkt"
                     /locus_tag="OO544_001943"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCR6767551.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKDIYHAWDAREKGEKAQQRWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPLLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEITRGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(8838..9881)
                     /locus_tag="OO544_001944"
     CDS             complement(8838..9881)
                     /locus_tag="OO544_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HCR6767552.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAALAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQMIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(10007..10582)
                     /gene="nudK"
                     /locus_tag="OO544_001945"
     CDS             complement(10007..10582)
                     /gene="nudK"
                     /locus_tag="OO544_001945"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HCR6767553.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKTVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQTSHLMD"
     gene            complement(10650..12629)
                     /gene="aegA"
                     /locus_tag="OO544_001946"
     CDS             complement(10650..12629)
                     /gene="aegA"
                     /locus_tag="OO544_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HCR6767554.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHLTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRLVPIEG
                     SEFVMPADAVIMVFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            12835..14535
                     /gene="narQ"
                     /locus_tag="OO544_001947"
     CDS             12835..14535
                     /gene="narQ"
                     /locus_tag="OO544_001947"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HCR6767555.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSSVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYLSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGENWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            14699..17812
                     /gene="acrD"
                     /locus_tag="OO544_001948"
     CDS             14699..17812
                     /gene="acrD"
                     /locus_tag="OO544_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HCR6767556.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGIGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNKIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVA
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(17911..17970)
                     /gene="ypfM"
                     /locus_tag="OO544_001949"
     CDS             complement(17911..17970)
                     /gene="ypfM"
                     /locus_tag="OO544_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HCR6767557.1"
                     /translation="MIERELGNWKDFIEVMLRK"
     gene            18352..18708
                     /locus_tag="OO544_001950"
     CDS             18352..18708
                     /locus_tag="OO544_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HCR6767558.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
     gene            18712..19839
                     /gene="dapE"
                     /locus_tag="OO544_001951"
     CDS             18712..19839
                     /gene="dapE"
                     /locus_tag="OO544_001951"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HCR6767559.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIDRLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            19867..20067
                     /locus_tag="OO544_001952"
     CDS             19867..20067
                     /locus_tag="OO544_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HCR6767560.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(20177..20875)
                     /gene="ypfH"
                     /locus_tag="OO544_001953"
     CDS             complement(20177..20875)
                     /gene="ypfH"
                     /locus_tag="OO544_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR6767561.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIEMVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(20949..>21745)
                     /gene="tmcA"
                     /locus_tag="OO544_001954"
     CDS             complement(20949..>21745)
                     /gene="tmcA"
                     /locus_tag="OO544_001954"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HCR6767562.1"
                     /translation="ALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLAA
                     TLRGRRVSRIAVHPARQREGVGQQLIASALQYRPGLDYLSVSFGYTGELWRFWQRCGF
                     VLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGETLPVDP
                     LNNAVLSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNTSDAQL
                     CTTLKLSGRKMLLVRQREEAAQALFALNEVRTERLRDRITQWQFFH"
BASE COUNT         5090 a         5816 c         5646 g         5193 t
ORIGIN      
        1 gacagcatcg ccacgcgcgg ctcttctccg gtgagggcgc gccaggtttc ggcactggca
       61 agcgcgatat ccgccagttg cgccgccgtc ggctgtggca ccacgctgca atcggcaaag
      121 cccaacgccg gaccgctgta ctgtggcagc atcaggaaaa tggatgagag cgttttacag
      181 cctggctgca agccgataat acgtaatccg gcacgcagca cattcgccgt ggaagagagg
      241 ttgcccgcga tacagacatc cgctttaccg gcgctgacca ttgctgcggc gaacatcagc
      301 ggatcggtaa gtttttccag cgcatccggc ggcgtttttt cgcccgcgcg ggccagccag
      361 cgatgagcaa attcttcccg cattgcgagg ttgccatgcg gatcaatcac ctgtagcccg
      421 tccatcgcca cgccgtgact gagcgcaaac tgacgaagtt caaacggatt agcgaccaga
      481 atgggcgttg ccagaccttg ttgatgtaaa tattgcgcag ctttcagcac acgttgatct
      541 aacgcatccg gaaaaaccac tctggcgggc gctcgcaacg ccagttcacg acaacgttca
      601 ataatcattg cgtttccccc agtcgttgtt ggatctgctt caccgttagc ggcggcttcg
      661 ttacgctcag gatcatcatt acgtagaccg tgctcgaaag gcggttcagc gcctgcaaga
      721 tgtccgggcg taatacttca aagctgcggg tgataaacac ctgcgccgct acggtttcgg
      781 tttcccgcac tttggtacgt agcagattga gcaacgccgc atcgcgcccg tggctggctt
      841 ccggcaccag atgatcgtgg tcgagatagc gcagcggctg gtgagaaagt cgatgcagat
      901 cttcgtcaga aagcccgaca atcgcctggc agcccagcgg ttcccccagc gcatcggcgc
      961 gcataatgtt gccaagacgc gaacggatat ccgccagcca cggctgccac ggttccgcca
     1021 gttcaatttg cagccacact gccagcgcaa tggtgctgtc gagaaccgcg cgaaaaccca
     1081 gacgcggatc gcttttggcg accatttttt ccgccgacag gtgagtcagc gtatccggct
     1141 tttttgccac cggctggcgg cacagttcgc agcacgcctg cggatgttca tcgctactgg
     1201 tcagcccatg aacgggctgc ggctgctgct gttcatcgtc aacaaacagg cggccctgct
     1261 cgtcaataaa cttgatgcgc agatggcggc tttccagcaa ctcccgggca gagggcgtca
     1321 ggcgactgtc cgcagggaga tggatctctg cgccttcgct gagcgtatgg ttcgctctta
     1381 gccatgcttc ggtgatgaaa tctttcatag ggattgccag ttagccggcc aggcgacata
     1441 caggaatttc acgctggatg tcgtaccaaa ttcgatgctg gagcctttcg ggataaacat
     1501 cacgtcgcct gctttggcga tcatggtttc gccttcgtgg cgaacatgga gctccccttc
     1561 cagcaccatg tcgatttcgt cgtagttcag ggtccacggg aagaatgcgt tctcccactg
     1621 cataaaaccg gcggccatgc tgctgccgtc gtctccggtc acaagatcag tcaagcccac
     1681 gcaatgcggt tgtgcgccat caaaacgacc aaacttgacg ctgctgccat caatcacttt
     1741 gatgccgcct ttgccggtca ccgatttgaa gctcggctgc attgcgccct gctccagcga
     1801 ctgtttttcc ttcatcactt tttccatcag ctgtgcgacc aggctttcgg taaactggcc
     1861 ttccggcagc tgggcaatga tggtttcgcg gatgcgctgg ctttcggttt tgtcggctgg
     1921 cacgctggcg ggcacggacg ccgttaccgg gatcgattca tcacattcgg tgatggtaaa
     1981 acccagtaga tccgccactt tacgggcttc cggggtaata atgctggcgc gcagaaccac
     2041 ggacattgcc tgttcgccgc gcgcgtgtgc ttcacgaata tcattcgctg tgatgagttt
     2101 tttcacctgc ttcctcctgt ttggtgagag atgccagata atccaccagt tgctgcacgc
     2161 tttgcgggtc gtggctgttt agctcgaaaa tcggctcttc gaaacccgtc tcaagcaata
     2221 actttcgcgt tgcggcgacg tcagcatctg gcatgtccgt tttactgatg acggcgattt
     2281 gccgtttgct gacgccaata tccagcaacc cggcaggcaa ccgactttcc gggtcatttg
     2341 cgccatggac atagatcagc atgtctacat cctgtagcgt ggtaattaag gcgtgatacc
     2401 agcggggatg gctaaaatat tcacccggcg tatcaatatc gcccttatca ttaaattcca
     2461 cggcctgtgt ttttctggcg agggtataat tcccctgcaa cgcattaaat aacgttgttt
     2521 ttcctgcccc gacgctaccc acaaacgcaa ttcgtttcat gaatgcctcg tattaacttt
     2581 tggtcatttc acaaagcgta taatttaata agcgacctaa accgctgacg gtttgtgata
     2641 acgcttcttc tactgcgccc accgagccat aaatcaccaa tgcaccgctg aagcggtcga
     2701 gaaaaccgat atgcacatca gcggctttca gcgctaaatc accgacgatc atcgccgttt
     2761 cgccgggagt tagcgtcata atgccaatta cgcccgcatc cggaacgccg attttcttcg
     2821 ccagttcctc accgggatgc gcgatgagat gcgccagtgt gacctgtttg ccgggcacaa
     2881 attcctgaat gatgcgttct ttatccatga gtcgccgcct gtgaaaaact ttcgtgtatc
     2941 gtgacaattt ttcgtcagag taaataacaa aattcggcaa gcgcgtttaa aaggtgaggt
     3001 agatcactaa gaatggagag atattcgctg tggtgcataa actttcatat gcaacgcatg
     3061 aatataaata aaataatatc gagatattat atagactgct ttttttatta ttcgcaaact
     3121 cagtaataag ctttactaat atattgcagc caaaccgctt accctgaata ttcagggtaa
     3181 gcgtgagagt taaaaaagaa ttacagcggt tgggtttgcg cttcaaccac ggccagcgcc
     3241 accatgttga cgatacgacg caccgatgcg atcggcgtta acacgtgaac cggtttcgcc
     3301 acgcccatca gcaccggacc gacagtcaca ccttccgagc tggaaacgcg cagtaagttg
     3361 taactaatgc gggcagcttc catgttcggc atcaccagaa tattggcaga acctttcaaa
     3421 gggctgtccg gcatacggtc gttgcgaatc gcttccacca gcgccgcatc gccgtgcatt
     3481 tcaccatcaa tcatcagttc tggcgcacgt tccctgacca gttccagcgc ctgacgcatt
     3541 ttgctcgacg acgggcagtc agaagaacca aagttggagt gcgacaacag agcaacgcgc
     3601 ggttcaatac cgaaacgacg gacagtttct gccgccatca aggtgatctc cgccagctct
     3661 tctgcgtctg gttcgtcatt gacgtaggta tcggcaataa aggtgttacc actcggcagc
     3721 agcagcgcgt tcattgcacc tgcggtgtga acgccatcgc gataaccaaa gacatttttc
     3781 accacgctaa aatgttcatg ataatcaccc accgtaccgc aaatcattgc atcggcttcg
     3841 ccacgctgaa ccatgatcgc gccgatcact gtcgggttac tgatcagcgc ccgctgcgcc
     3901 tgttcctgag tgacgccgcg acgcttcatg atctggaagt attcggtcca gtactcttta
     3961 aagcgcggat cggattcgtt attgacgatc tcaaaatcaa cgcccgcttt gatctgcaag
     4021 cccagtttct gaatgcgcat ttcgatcacg ttcggacgac cgataaggat cggtttcgcc
     4081 agtcccagcg ttaccagttc ctgagtggca tgcagaacgc gcgcctcttc cccttccggc
     4141 agaactacgc gcttcggcgc tttgcgagcc tgggagaaaa tcggcttcat aaacaggttg
     4201 gttttgtaaa cgaactcagt cagcttgtcg atgtagacgt cgaaatcagc aatcggacgc
     4261 gtcgccacgc ccgactccat cgccgctttc gcgaccgcag gagcgatctt aacgatcaag
     4321 cgcggatcaa acggttttgg aatgatgtat tccggaccaa agctcagatc ctgatcgcca
     4381 tacgctgaag ccaccacttc gctctgttcc gcatgggcca gttctgcaat cgcgcgtacc
     4441 gccgccagtt tcatctcttc gttgatggcg gttgcgccaa cgtccagcgc gccacggaag
     4501 atgaacggga agcaaaggac gttgttgacc tggtttggat agtcagaacg accggtgcag
     4561 atgatggcat ccggacgcac ttctttcgcc agcggcggca gaatttccgg ttccggattc
     4621 gccagcgcca gaatcattgg tgcccgagcc attttcttca ccatttcctg ggtcagcact
     4681 ttcgggccgg aacagcccag gaaaatatcc gcaccttcaa tcacatcatc gagggtacgt
     4741 ttgccgtcat ccaccaccgc atatgcggct ttggtttccg ccatgtttgg ctcacggcct
     4801 tgatagataa cgccttttga atcgcaaacc acgatgttat gtttttgcag acccagcgct
     4861 accagcagat tcatacaggc gattgctgcg gcacccgcgc cggaaaccac catccgcacg
     4921 tcggagatgt ttttctccac cacgcgcaag ccgttgagga tggcggcagt gctgatgatt
     4981 gccgtgccgt gctgatcgtc gtggaatacc ggaatattca tccgctcgcg cagtttctgt
     5041 tcaatataga aacattctgg cgctttaatg tcttcgaggt tgattccgcc gaaggtcggt
     5101 tcgagcgcag cgacaacttc aataaatttg tccgggtcga gttcatcaac ttcaatgtca
     5161 aatacatcaa tcccggcgaa tttcttaaac agaacgccct tgccttccat caccggtttg
     5221 ccagccagcg cgccaatgtt gcctaacccc agcaccgccg taccgttaga gatcaccgcc
     5281 accaggttac cgcgggcggt atatttgtag gcttttaacg ggtctttttc gatttcaaga
     5341 caaggtgcgg caacgccggg tgagtaggcc agcgccagat cgcgctgcgt tgccagaggc
     5401 ttggttggag aaacctggat tttccctgga actggaaatt catggaaatc aagtgcactt
     5461 tgttttaact ggtcatccat ttggttgttc ctttcacgta acgttcacaa ataaagtgtg
     5521 tgggcaacac cccctgccca caacgtggcg cacattatta ccttgccgga gtctacagac
     5581 tttgagcaag tccaaactct caccattaat ataatgtttt ggtaataatc ctataacact
     5641 gatgttacct gcttaatcca gcaataccat gcctgtctgc tatgcttttt tgatgcgttt
     5701 agcgaaattt ctcagaagtg tgaattaacg cactcatcta acactttact tttcaaggag
     5761 tatttcctat gaacgagtta gacggcatca aacagttcac cactgtcgtg gcagacagcg
     5821 gcgatattga gtccattcgc cattatcatc cccaggatgc caccaccaat ccttcgctgt
     5881 tactcaaggc tgccggatta tcacaatatg agcatttaat agacgatgct atcgcctggg
     5941 gtaaaaaaaa tggcaagacc caggaacaac aggtggtcgc agcgtgtgac aaactggcgg
     6001 tcaatttcgg tgctgaaatc ctcaaaatcg tacccggtcg cgtgtcaaca gaagttgatg
     6061 cacgcctctc ttttgataaa gaaaagagta ttgagaaggc gcgccatctg gtggacttgt
     6121 atcagcaaca aggcgttgag aaatcacgca ttctgatcaa gctggcttcg acctgggaag
     6181 gaattcgcgc ggcagaagag ctggaaaaag aaggtattaa ctgcaacctg acgctgctgt
     6241 tttcttttgc acaggcacgg gcctgtgcgg aagcaggcgt ttttctgatt tcgccgtttg
     6301 tcgggcgtat ttatgactgg tatcaggcac gcaagccgat ggacccgtat gtggtggaag
     6361 aagatccggg cgttaaatcg gtgcgcaata tctacgacta ctataagcaa caccactatg
     6421 aaaccattgt gatgggcgcg agcttccgtc gcaccgaaca aatcctcgcc ttaaccggct
     6481 gcgatcgact gactatcgca ccgaatttgc tgaaggagct gcaggaaaaa gtttcgccag
     6541 tggtacgtaa attaatccca ccttctcaga cgttcccacg cccagctccc atgagcgaag
     6601 cggagttccg ttgggagcac aatcagtatg cgatggcggt agaaaaactg tctgaaggca
     6661 ttcgtctgtt cgccgttgat caacgcaaac tggaagatct tcttgccgcc aaactataaa
     6721 ccagccacgg agtgttatat gtcccgaaaa gaccttgcca atgcgattcg cgcactcagt
     6781 atggatgcgg tacaaaaagc caattctggt catcccggcg cgccgatggg catggctgat
     6841 attgccgaag tgctgtggaa cgattttctt aaacataacc ctaccgaccc aacctggtat
     6901 gatcgcgacc gctttattct ttccaacggt cacgcgtcga tgctgctcta cagtttgctg
     6961 catctgaccg gttacgacct gccgctggaa gagctgaaga actttcgtca actgcattcg
     7021 aaaaccccag gccacccgga gattggttat acgccaggcg ttgaaaccac caccggtcca
     7081 cttggacaag gtttggcgaa cgccgtcggg ctggcgatag cagagcgtac actggcggcg
     7141 cagtttaacc agccagacca tgagatcgtc gatcacttca cctatgtgtt tatgggcgac
     7201 ggctgcctga tggaaggtat ttcccacgaa gtctgttcgc tggcaggcac gctgggactg
     7261 ggcaagctga ttggttttta cgatcacaac ggtatttcca tcgacggtga aacagaaggc
     7321 tggtttaccg acgatacggc aaaacgtttt gaagcctatc actggcatgt gatccatgaa
     7381 atcgacggtc acgatccgca ggcggtgaag gaagcgatcc ttgaagcgca aagcgtgaaa
     7441 gataagccgt cgctgattat ctgtcgtacg gtgattggct ttggatcgcc gaataaagca
     7501 ggtaaggaag aggcgcacgg cgcaccgctg ggggaagaag aagtggcgct ggcacggcaa
     7561 aaactgggct ggcaccatcc gccatttgag atccctaaag atatttatca cgcctgggat
     7621 gcccgcgaaa aaggcgaaaa agcgcagcag aggtggaatg agaagtttgc cgcctataaa
     7681 aaggctcatc cgcaactggc agaagagttt acccgtcgga tgagcggtgg tttaccgaag
     7741 gactgggaga aaacgactca gaagtatatc aatgagttgc aggcgaatcc ggcgaaaatc
     7801 gccacccgta aggcttcgca aaatacgctt aacgcttatg gccccttgct accggagctg
     7861 ctcggcggtt cggcggatct ggctcccagc aacctgacca tctggaaagg ttctgtttcg
     7921 ctgaaggaag atccggcggg caactacatt cactacgggg tgcgtgaatt tggcatgacc
     7981 gctatcgcca acggcatcgc gcaccacggc ggctttgtgc cgtataccgc gacgttcctg
     8041 atgtttgttg aatacgcccg taacgccgcg cggatggcgg cactgatgaa agcgcggcag
     8101 attatggttt atacccacga ctcaattggc ctgggcgaag atggtccgac gcaccaggct
     8161 gttgagcaac tggccagcct gcgcttaacg ccaaatttca gcacctggcg accgtgcgat
     8221 caggtggaag cggcggtggg ctggaagctg gcggttgagc gccacaacgg accgacggca
     8281 ctgatcctct caaggcagaa tctggcccag gtggaacgta cgccggatca ggttaaagag
     8341 attactcgtg gcggttatgt gctgaaagac agcggcggta agccagatat tattctgatt
     8401 gccaccggtt cagagatgga aatcaccctg caagctgcgg agaaattagc aggagaaggt
     8461 cgcaatgttc gcgtggtttc cctgccctcg accgatattt tcgacgccca ggatgaggaa
     8521 tatcgggagt cggtgttgcc ttctaacgtt gcggctcgcg tggcggtgga agcaggtatt
     8581 gccgattact ggtacaagta tgttggtctg aaaggggcaa ttgtcgggat gacgggttat
     8641 ggggaatctg ctccggcgga taagctgttc ccgttctttg gctttaccgc cgagaatatt
     8701 gtggcaaaag cgcataaggt gctgggagtg aaaggtgcct gatagtgatc gccggatgct
     8761 gattgccgga tgcgacgctg acgcgtctta tccggcctac agcgtcttat ccggcctaca
     8821 tgtccccgcc attttgttta acgggtgatc cacaacgtgg gccaggcgtc tggcccatgc
     8881 cagttatcgc aggtgggttc tgcggcgtaa cgcaccaggc gaaaacgctg accgtcaaag
     8941 cgccagcgcg cctgaatgcc acaatcgctt aatccgcgcc ctttcgctaa ggtcaccagt
     9001 tcacgcgatt tctcatcaaa tgttgcgttc atcagttcca gttcattcgt ctcctgcccg
     9061 ttgttgaacg gcaaacgcaa ccgaaccggg cgcgaggcta gcggcttttt acgcgacaca
     9121 atccatgcca aatcaatggt gttataggcc cctgcctcac agctgatcat catcagcgct
     9181 ttatcatcag tcagcgcagt gacattcacc tcacgacgta acggatcaag cgagcagcgc
     9241 agaccattca tccgccagtt tccataatcc agcaaatcgt tgcgttcttc gagtgagagt
     9301 ggcgtcggcg ttgggttaac caccgcgacc tctttcagcg caggcgcggg cggtacgctg
     9361 agcggcggtt cgtccccttt cttgatccac gcggtttcac tgccaacgcg cttttgctga
     9421 gcatcaataa acaacaacgc cgcttttaag ccactcagag aaatggtctg attgccgtcg
     9481 cgtaaggtga tcgccttccc ttcctgaatc atctgcaaaa acgcggtgat ggttgccgta
     9541 tcatcggtta ctaataacca tggtgaaatc cgccacttgt caccacttaa cgccaacggc
     9601 tcaccatcta acagcagccg tggcgctatc tctccttcag acgcatccgg cgacttcaac
     9661 ccgccgcgct caatacgtaa aacggcatcg gtatgcgccc cggcgctgcg gctcagggtc
     9721 atcaccagtc cattatgatc gcccgtattg cgcgccacgc agaagttttg gttattacag
     9781 gtgacctgcc agtcggaaaa cgcccgttgc gctagtgccg cccacaccaa agacgtcggc
     9841 aacaaagcga aaaaaaagag aaggaaaatg cgatagcgca tggacggtac gaccccagaa
     9901 gttcaaacaa acagcacaag tcgtatcttc gttgcgcagg tagcgcggct caatcagatt
     9961 tgtcggattg agccaataat aaacaacgaa atattattgt tatttttcag tccattaaat
    10021 gtgacgtttg caaatagtta agcaataaca ccgtcttacc gtcacgtatc tcgccggttt
    10081 tgatcatctc cagcgcctgg ctgaacggca gctcaagcac ttcaatatct tcatcttcaa
    10141 caccgccacc ggcgttagcg cgctgattgt cactgtattc ggcgataaaa aagtggatta
    10201 gctcagtcac accgccaggc gacatataca gttcaaataa tttgcgcact tcgcccactt
    10261 catagcccgt ctcttcaatc gcttctttac gaatgcacac ttccggttcg tcgttatcca
    10321 gcagcccggc gcaggtttca atcagctgcc cgctttcatt gccattaacc caggtagcga
    10381 cacggaactg acgaatcaga accacggtct ttttcttcgc gttgtacagg aggatcgtcg
    10441 caccattacc gcgatcgtaa acttcacgtt tatggcggat aacttcgcca tctttgcgcg
    10501 tgagatcgta agtaatgttg tgcagggtga aatagttatc ggagagaatt ttgtctttaa
    10561 tgagggtgat ttgttgcgtc ataccgactc cacagcgcga aatgaacaat tatattacgc
    10621 tgtgaagtcg ggtttgtctg cgcaggctat cagtgagatt tgactgattt tacccccagc
    10681 cagtcaataa tcccctgtgc cgcatgacgt ccttctgcca ttgcggtaac caccagatcc
    10741 gcaccacgca cggcgtcgcc accggcgaag attttcggat tggtggtctg gtaacggtac
    10801 tggctttcca catccgcgat gatgcggccc catttgtcta ccgttacacc gtgcgactcc
    10861 agccacggca tcccgtgtgg attgaagcca aacaccataa tcaccgcatc ggctggcatg
    10921 acaaattcac tgccctcaat cggtacaagt cgccggcgcc cctgggcatc cggctcgcca
    10981 agacgcgtgc gcaggaaacg aattccgcaa acgtgacctt gctcattcag ctcaagcgcc
    11041 accggctgga cgttaaattc gaagttggcc ccctcttcgc gggcgttctt cacctctttc
    11101 ttcgagcctg gcatgttagc ttcatcacga cgataagcgc aggtgacgtt actcgcgccg
    11161 tggcgcagcg cggtacgcac acagtccatc gcggtgtcgc cgccgcccag taccacgacg
    11221 ttgagtccgg cggtattgat aaacggctct tccggtagct cttcgagtcc catcacctgt
    11281 ttagtgttgg cgatgaggaa cggcagcgcg tcataaacgc ccggcgcatc ttcattgggt
    11341 aaacccgctt tcatggaacg gtaagtgcct acgccaacga agaccgcgtc gtattgttcc
    11401 aaaagcgaat ccaaagagac atctttaccc acttcacaat tgagttcgaa gtgaatcccc
    11461 atcgcgctga agatttcccg acggcgtgcc agcagggatt tatccagttt gaaagaagga
    11521 atgccgaaag tgagcaagcc accaatttct ggatggcgat cgtacaccgt caccgccacg
    11581 ccattgcggg tcagaacatc cgcacaggcc agccctgccg gacccgcacc gataatcgcc
    11641 acccgcttgt ccactttggt gacatggctt aagtcaggac gccaaccttt cgccaacgcc
    11701 tgatctgaaa tgtagcgttc aatgttgccg atagttaccg cgccgtgctc atcgcgaata
    11761 gtacaggcac cttcacacaa acggtcctgc ggacaaacgc gtccggtaat ttccggcagg
    11821 gtgttggtca ggtgagaaag ctcgacggcg gcgtcgatgt ttccggcttt caccagttca
    11881 atccactgcg gaatatggtt atgcagcggg caggtccatt cacaaacgct gtgctcgccg
    11941 catttaaggc agcgcgaggc ttcccgttgt gcctgatcgg cacggaatgg cagataaatt
    12001 tcatcaaaac cggttttgcg cgcttcaatc gccagtttat ccggctcgcc acgcgcgggc
    12061 gttgcctgca tttgttcgac tttactcatt accggcattt cttgtgcagc ggtactggca
    12121 tgccacggtt gatgctcctg acgtgcggtg cgcaagcggc gggatttcgc catgccggag
    12181 agtgcgacgt cagtgaccag ttgcagcgcg tccgccgggc aattctcgac acaggcagga
    12241 ccgttttcgc gccctgcaca aaggtcgcat ttatgcgccg tggcttttac ttttcctgcc
    12301 gcgacgggtg tcaggacgat ttgcatcgta ccaaacggac aggccaccac gcaggattta
    12361 cagccaatac acttttgctg attgacctga atgctgtcat caacgtggct gattgcgcca
    12421 ttagggcagc tacgggcgca gggcgcatct tcacaatggt gacaggtcac tgcactacgt
    12481 tgctgctgat gtttgataac cgtaattcgg ggatgaaaat ggtgttggct caggacatgt
    12541 tgctcatcat tgtgagccat gacacaggcg atttcacaag catgacaacc cagacattgc
    12601 tgactgttgg ccataataaa acgattcata acgaccttct tttttggttg taaaaacctt
    12661 attctttata tgagtgttgt tattacccga cttacagggg aatcggcaat gttcatgtgc
    12721 ccagaataag taactatttc gctataaact gtggcagatc aaataatccc atcactgact
    12781 aaattgcgtt ttagcgaact ggaacattaa tgattttttg tggagaagac gcgtgtgatt
    12841 gttaaacgac ccgtctcggc cagtctggcc cgggcctttt tttacattgt gctgctgtcg
    12901 attctttcca cgggtatcgc tctgctaact ctggcgagca gtttgcgcga cgctgaggct
    12961 atcaatattg ccggatcgct gcgtatgcag agttaccgcc tgggctacga cttgcaaagt
    13021 ggcagtccac aactcaatgc acatcgccag ttgtttcagc aggcactgca ttcatcggta
    13081 ttaaccaatc tcaacgtctg gtatgtgcca gaagcagtaa aaactcgcta tgcgcatctg
    13141 aatgccaact ggctggagat gaataatcgg ctcagcaagg gcgatttgcc gtggtatcag
    13201 gccaatatta ataattatgt taatcagata gacctgttcg tgctggcttt acagcactac
    13261 gctgaacgca aaatgctgct ggtggtggcg atttccctgg ctggcggtat cggtattttc
    13321 acgctggtct tttttacttt gcgccgtata cgccatcagg tggttgcccc gctgaatcag
    13381 ctggttaccg ccagtcaacg tattgaacac gggcagttcg actcgccgcc gctggatacc
    13441 agtctgccga atgagcttgg tctgcttgca aaaaccttta accagatgtc gagcgagctg
    13501 cataaattgt acctttcgct ggaggcgtca gtagaagaaa agacccgcga tctccacgag
    13561 gccaagcgtc gtctggaggt gttgtatcaa tgttcgcagg cgctgaacac aagccagatt
    13621 gatgtgcatt gtttccgcca tattttgcag attgttcgcg acaatgaagc ggctgaatat
    13681 ctggagttaa atgtcggtga aaactggcgg attagcgaag ggcaaccaaa cccggaattg
    13741 ccgatgcaga ttttaccggt gacaatgcaa gagacggttt acggcgaact gcactggcaa
    13801 aatagtcacg tttcatcatc agaaccgctg cttaacagcg tttcgtcgat gctgggacgc
    13861 ggtttgtact ttaatcaggc gcaaaagcat tttcagcagt tattgttgat ggaagaacgt
    13921 gcaaccatcg cccgcgaatt gcacgactcg ctggctcagg tactttctta cttacgtatc
    13981 cagttgacgt tactgaagcg ttcgataccg gaagataacg ccaccgcaca aagtatcatg
    14041 gccgactttt cccaggcgtt gaatgatgct tatcggcagt tacgcgagct gttgactact
    14101 ttccgcctga cgctgcagca ggcggatctc ccctccgcgt tgagggaaat gctggatacg
    14161 ttacaaaatc aaaccagcgc caaactgacc ctcgactgcc gtctgccaac cctggcgctg
    14221 gatgcgcaaa tgcaggtgca tttgttgcaa attattcgcg aagcggtgct gaatgcgatg
    14281 aagcacgcca acgccagcga aatcgccgtc agttgcgtca ccgcgccgga cggcaatcac
    14341 acggtttata tccgtgataa cgggattggt atcggtgaac cgaaagaacc cgaaggtcat
    14401 tatggtctga atatcatgcg cgaacgcgcg gaacggctgg gtgggacgct gactttttca
    14461 caaccttccg gcggcggcac gttagtgagt attagctttc gctctgcgga gggtgaggaa
    14521 agtcagttga tgtaatgcct cctactgacc aaagaatact tgcacttaag gttcagtata
    14581 aaagggcatg ataatttaca ttaactcctt tttttctcca cgattggctc gtaccttgcc
    14641 gctacagtga agcaagtcaa gcctacaacg atacgcagaa acacgaggtc ctcttttaat
    14701 ggcgaatttc tttattgatc gccccatttt tgcctgggtg ctggcaatcc tgttgtgtct
    14761 gacagggacc ctggcgattt tttcattgcc cgttgaacaa taccccgatc tcgcgccacc
    14821 gaatgtgcga gtgaccgcta actatcccgg cgcatcggcc cagacgctgg aaaacaccgt
    14881 gacccaggtt atcgagcaaa atatgaccgg cctcgataat ctcatgtata tgtcatctca
    14941 gagcagtggc atcggtcagg catctgtcac tttaagtttt aaagcaggca ccgatccgga
    15001 cgaagccgtg cagcaagtac aaaaccagct gcaatcagcc atgcgaaagt taccgcaggc
    15061 ggtgcaaaat cagggcgtga cggtgcgtaa aaccggcgat accaatattc tgaccattgc
    15121 tttcgtctct accgatggtt cgatggataa acaggatatt gccgattatg ttgccagtaa
    15181 tattcaggac ccgttaagcc gcgtgaatgg cgtcggggat atcgatgcct atggttcgca
    15241 atattccatg cgtatctggc tggacccggc gaaactcaac agtttccaga tgacggctaa
    15301 agatgtcact gatgccattg agtcacagaa cgcgcagatt gcggttgggc aacttggtgg
    15361 tacaccttcc gtcgataagc aggcgctcaa cgccaccatt aacgcccagt cactgctgca
    15421 aacaccagaa cagttccgcg atatcacctt gcgggtgaat caagacggct cagaggtaag
    15481 gctgggcgat gtcgccaccg tcgaaatggg ggcggagaaa tacgattatc ttagccgctt
    15541 caatggtaag ccagcctccg ggctgggggt aaaactggcc tccggcgcaa acgaaatggc
    15601 gacagcggag ctggtgctca atcgtctcga cgagctggcg cagtacttcc cgcacggact
    15661 ggaatacaag gtggcgtatg aaaccacctc gtttgttaaa gcctccattg aagacgtggt
    15721 gaaaacgctg ctggaagcta tcgccctggt tttcctcgtt atgtatctgt tcctgcaaaa
    15781 cttccgcgcc acgctgatac ccactatcgc cgtgccggtg gtgttgatgg gaaccttctc
    15841 cgtactttac gccttcggtt acagcgtcaa caccttaacc atgttcgcga tggtactggc
    15901 gatcggtctg ctggtggatg acgcaatcgt ggtggtggaa aacgtcgaac gtattatgag
    15961 tgaggaagga ctcactcctc gcgaagccac acgtaaatcg atggggcaga tccagggggc
    16021 actggtcggg attgcgatgg tgctttcggc ggtatttgta ccaatggcct tcttcggcgg
    16081 caccaccggt gccatctatc gccagttctc tattaccatt gttgcggcga tggtgctgtc
    16141 agtactggta gcgatgatcc tcactccggc tctgtgtgcc acactactta agccactgaa
    16201 aaaaggtgag catcacgggc aaaaaggctt ttttgcctgg tttaaccaga tgtttaaccg
    16261 caacgccgaa cgctacgaaa aaggggtggc gaaaattctc caccgcagcc tgcgctggat
    16321 tgtgatttat gtcctgctgc ttggcggcat ggtgttcctg ttcctgcgtt tgccgacgtc
    16381 gttcttaccg ctggaagacc gtggcatgtt tactacctcg gtacaattgc ccagcggttc
    16441 aacccaacaa cagaccctga aagtcgttga gcaaatcgag aaatattact tcacccatga
    16501 aaaagacaac atcatgtcgg tgtttgccac cgttggttct ggccctgggg gtaacgggca
    16561 aaacgtggcg cgaatgttta tccgcctgaa agactggagc gaacgcgaca gtaagaccgg
    16621 cacctcgttt gccattatcg agcgtgcaac aaaggcgttt aacaagatta aagaagctcg
    16681 cgttatcgcc agcagcccgc cagcaattag cggtcttggt agttctgcgg gttttgatat
    16741 ggagttgcag gaccacgctg gagcaggtca cgatgcgctg atggcagcac gtaaccagtt
    16801 gctggcgctg gcggcggaaa acccggagct aacccgtgtg cgccataacg gcctcgacga
    16861 cagtccgcag ttgcagattg atatcgacca gcgtaaagct caggcgctgg gcgttgctat
    16921 cgacgatatt aacgacacac tgcaaaccgc ctggggttcg agctatgtga atgactttat
    16981 ggatcgcggt cgcgtgaaga aagtctatgt gcaggcagct gcgccgtatc gcatgctgcc
    17041 agatgacatc aatctctggt atgtccgaaa taaagatggc ggcatggtgc ccttctctgc
    17101 tttcgcgacc tcacgctggg aaacaggctc gccgcgtctg gaacgctata acggttattc
    17161 tgcggttgag attgttgggg aagccgcacc gggggtcagt accggtacgg cgatggatat
    17221 tatggaatcg ttagtgaagc agctgccaaa cggctttggt ctggagtgga cggcgatgtc
    17281 gtatcaggag cgactttccg gcgcgcaggc tccggcgctg tacgccattt ccttgctggt
    17341 ggtattcctg tgtctggctg cgttgtatga aagctggtcg gtgccgttct cggtaatgct
    17401 ggtcgtgccg ctgggggtaa tcggcgcgct gctggcaacc tggatgcgcg ggctggaaaa
    17461 cgacgtttac ttccaggtgg gcctgttaac ggtcattggt ttatcggcga aaaacgccat
    17521 cctgatcgtc gagtttgcta acgagatgaa ccaaaaaggc cacgacctgt ttgaagcgac
    17581 gctccacgcc tgccgtcagc gtttacgccc gattctgatg acctcgctgg catttatctt
    17641 cggcgtattg ccaatggcaa ccagcacggg tgccggttcc ggtggtcagc atgcggtggg
    17701 tactggcgta atgggcggga tgatttcagc cactattctg gctatttact tcgtgccgct
    17761 gttctttgtg ctggtgcgcc gccgcttccc gctaaagccg cgcccggaat aagcaataaa
    17821 aaaggcgaca tgccaatgtg tcgccttttt caactttccg attaagaacc tgctcagcgg
    17881 gttcttgctg tttatacttt gtctcaggaa ttacttacga agcataactt cgataaagtc
    17941 tttccagttc cccagttcac gttcaatcat aacaacctct cttataatta tgggtattct
    18001 acggaaacaa tataccgtgg tgaagctaat ttactcgatt gctgcgatga ctacctccgg
    18061 ggggacaaat cttatgtaaa tactatggtc ctgcggtgat gatttgtatg tgatacacag
    18121 caacatttcg agatattcat acggcatcta atactgattt aattcaggtt aaaatacaga
    18181 cagataacaa gatgaatatt cttaatgttt acgttaaaaa tgtttaatat tatttaatag
    18241 ttgttaattt gaatacttcg ataatgttat atttcctgat aatcatttgc aggcaaaatg
    18301 ttttcaccct taaatgagta tttattctca taaatcgaaa aaggattcat tatggttaca
    18361 ctttacggta tcaaaaattg tgacaccatt aaaaaggctc gccgttggct ggaagccaat
    18421 aacatcgact atcgttttca tgattaccgc gtcgatgggc tggacagcga attattgaac
    18481 ggttttatca acgaattagg ttgggaggcg ttactcaaca cccgtggtac aacctggcgt
    18541 aaactggacg aaaccacccg caataaaatc accgatgcgg cctcagcggc ggcattaatg
    18601 actgaaatgc ctgcaattat caaacgtcca ttgctctgcg cgcccggtaa gcctatgctg
    18661 ctgggtttca gtgattccag ttatcagcaa tttttccatg aggtgtagtc tatgtcgtgc
    18721 ccggttattg agctgacaca acagcttatt cgccgccctt ccctgagtcc tgatgacgca
    18781 ggatgccagg ctttgttgat tgatcgtttg caggcgatcg gttttaccgt tgaacgcatg
    18841 gactttgccg atacgcagaa tttttgggca tggcgtgggc aaggtgaaac attggccttt
    18901 gccgggcata ccgacgtggt gccgcctggc gacgccgatc gttggatcaa tccgccgttt
    18961 gaacccacca ttcgtgatgg catgttattc gggcgcggtg cggcagatat gaaaggctcg
    19021 ctggcggcga tggtggtagc agcagaacgt tttgtcgcac aacatcccaa ccatacggga
    19081 cgactggcat ttctgatcac ctctgatgaa gaagccagtg cccacaacgg tacggtaaaa
    19141 gtcgtcgaag cgttaatggc gcgtaatgag cgtctcgatt actgtctggt cggcgaaccg
    19201 tcgagtatcg aagtggttgg tgatgtggtg aaaaatggtc gtcgcggatc gttaacctgc
    19261 aaccttacca ttcatggcgt gcaggggcat gtggcctacc cacatctggc tgacaatcca
    19321 gtacatcgcg cagcaccttt ccttaatgaa ttagtggcta ttgagtggga tcagggcaat
    19381 gaattcttcc cggcgaccag tatgcagatt gccaatattc aggcgggaac gggcagtaac
    19441 aacgttattc cgggtgaact gtttgtgcag tttaacttcc gcttcagcac cgaactgact
    19501 gatgagatga tcaaagcgca ggtgcttgcc ctgcttgaaa aacatcaact gcgctatacg
    19561 gtggattggt ggctttccgg gcagccattt ttgaccgcgc gcggtaaact ggtggatgcg
    19621 gtcgttaacg cggttgagca ctataatgaa attaaaccgc agctactgac cacaggcgga
    19681 acgtccgacg ggcgctttat tgctcgcatg ggggcgcagg tggtggaact cgggccggtc
    19741 aatgccacta ttcataaaat taatgaatgt gtgaacgctg ccgacctgca gctacttgcc
    19801 cgtatgtatc aacgtatcat ggaacagctc gtcgcctgat gagtggttct gcaagaggaa
    19861 ataagcatgg actggctggc taaatattgg tggattctgg tgattgtctt tttggtaggc
    19921 gtcctgctga acgtgattaa agatctcaag cgcgtcgacc acaagaaatt tctcgccaac
    19981 aagccggagc ttcccccgca tcgtgatttc aacgataagt gggacgatga cgacgactgg
    20041 ccgaaaaagg atcaaccgaa gaagtaagct gagtgctcaa ccgtaggccg gataaggcgt
    20101 taacgccgca tccggcaatg gtgaacgatg cctgatgcga cgctcacgcg tcttatcagg
    20161 ccaggtaatc tgtcttttac atcatttcaa tcacatcatc atcgccaggc ttaccgccac
    20221 ttagcgcttc gtcgaaataa tgtttcggaa tggtataacg caagtgatcc agggcaaatt
    20281 gcatactgcg attgtcgatc gcatggccta aatcctccac gatatccagc gtcacgtcac
    20341 caccggcact gattaacgct tcctgcgccg caaccgcatg cgccagatcg ataaccggat
    20401 cttcaccgcc atgaatcagg tgaattgtcg tggctgtaga cgcagtttcc ggcaggctcg
    20461 catagcgtcc gttaaaagca atgacgcgtg aagcaaggcc cggttcggct ttaatgctct
    20521 ccagcgccat aatcgcgcct tgagagaaac cgattagcgc tgtggcgtta gcccctactc
    20581 cgctctgttt ctgccagtag cgcaccatct caataaacgt cggcatgatg gcatccacac
    20641 gcgcctggcg attatcttcc gtaataccct gcaccgaaaa ccactgacgc cccgccggat
    20701 taccgctcgg ctccgcgcct ccgacactca ccaccagcgc atcaggaaat agcggcgcaa
    20761 accagctgcc tatttccccc attgccaccg ggttatcccc gacaccgtgg aaaagcagta
    20821 acaactgttg cgcaggttta tccgggcttt gaacaacaaa atggtcatgt ttcatggcga
    20881 actccttaac tgatgtcatt aattttacgc cgctgactca caatgaccat gggagaaaat
    20941 tgaaggagtc agtgaaaaaa ttgccattgc gttatgcgat cgcgcagacg ctcagtgcga
    21001 acctcattaa gtgcgaacag cgcctgcgcg gcctcttccc gctggcgaac cagtaacatc
    21061 ttgcggcctg aaagtttaag tgtggtacat aactgcgcgt cactggtgtt tttctgtaaa
    21121 cgcccacgca gcgccggtaa tgccagttca ctggtttgta gcagacgcaa taagcaacct
    21181 aacgacgtta atagcggacg atgagcgaaa gcaaaaccgg ccagttcaag ccagtcgtcg
    21241 tcagaaagga cggcattgtt tagtggatca acaggaagcg tttcgccatt ccactgcgcg
    21301 agagcttgcg catcgcgacg taaacggtaa tgctcacgtt cagccagctg tttacccgca
    21361 tcactcatcg gtaacagtgc catcgccgta tagcaaccgc tgctggcttc acgatgatta
    21421 cccattcgca ccagcacaaa accgcagcgt tgccagaaac gccataactc cccggtgtaa
    21481 ccaaaactca ccgaaagata gtcgaggcca ggcctatatt gcaaagcgct ggcaatgagc
    21541 tgttgcccaa cgccttcgcg ctgacgcgcc ggatggactg caatccggct gacccgccgt
    21601 ccgcgcaatg tcgccgccag tggattgctg ccgtgcgccg ccagcgactg ggccaccaga
    21661 ttaccccgcg ggcgacgaaa acctgcccat accgcctgac tgagttcttg agataatccg
    21721 ccctcatcca ccagccacaa cgccc
//
LOCUS       DAJTZL010000060        21642 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.060, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000060 DAJTZL010000000
VERSION     DAJTZL010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21642
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.060"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..1126)
                     /gene="fsr"
                     /locus_tag="OO544_001955"
     CDS             complement(<1..1126)
                     /gene="fsr"
                     /locus_tag="OO544_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HCR6767563.1"
                     /translation="MAMSEQPQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIAD"
     gene            1344..2996
                     /gene="ushA"
                     /locus_tag="OO544_001956"
     CDS             1344..2996
                     /gene="ushA"
                     /locus_tag="OO544_001956"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HCR6767564.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTREDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMIGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(3033..3512)
                     /gene="ybaK"
                     /locus_tag="OO544_001957"
     CDS             complement(3033..3512)
                     /gene="ybaK"
                     /locus_tag="OO544_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HCR6767565.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMAAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(3716..4510)
                     /locus_tag="OO544_001958"
     CDS             complement(3716..4510)
                     /locus_tag="OO544_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HCR6767566.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPTCEALEERINEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQITMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            4648..4989
                     /locus_tag="OO544_001959"
     CDS             4648..4989
                     /locus_tag="OO544_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HCR6767567.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(5204..7708)
                     /gene="copA"
                     /locus_tag="OO544_001960"
     CDS             complement(5204..7708)
                     /gene="copA"
                     /locus_tag="OO544_001960"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HCR6767568.1"
                     /translation="MSQTIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARVVTADGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGESVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTIVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNEQQVGTKAIEAEITA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKTGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            7970..8902
                     /gene="glsA"
                     /locus_tag="OO544_001961"
     CDS             7970..8902
                     /gene="glsA"
                     /locus_tag="OO544_001961"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HCR6767569.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENVEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            8905..10197
                     /locus_tag="OO544_001962"
     CDS             8905..10197
                     /locus_tag="OO544_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR6767570.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGSLLLSLIVAMVMKRNKTV"
     gene            10322..10729
                     /gene="cueR"
                     /locus_tag="OO544_001963"
     CDS             10322..10729
                     /gene="cueR"
                     /locus_tag="OO544_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HCR6767571.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(10730..11188)
                     /locus_tag="OO544_001964"
     CDS             complement(10730..11188)
                     /locus_tag="OO544_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HCR6767572.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(11185..12102)
                     /locus_tag="OO544_001965"
     CDS             complement(11185..12102)
                     /locus_tag="OO544_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HCR6767573.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGTSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            12248..12925
                     /gene="fetA"
                     /locus_tag="OO544_001966"
     CDS             12248..12925
                     /gene="fetA"
                     /locus_tag="OO544_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HCR6767574.1"
                     /translation="MQENSPLLQLQNVGYLAGDAKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNRQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            12912..13691
                     /gene="fetB"
                     /locus_tag="OO544_001967"
     CDS             12912..13691
                     /gene="fetB"
                     /locus_tag="OO544_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HCR6767575.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(13754..14608)
                     /gene="cnoX"
                     /locus_tag="OO544_001968"
     CDS             complement(13754..14608)
                     /gene="cnoX"
                     /locus_tag="OO544_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HCR6767576.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNFTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGMVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(14669..15478)
                     /gene="ybbO"
                     /locus_tag="OO544_001969"
     CDS             complement(14669..15478)
                     /gene="ybbO"
                     /locus_tag="OO544_001969"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HCR6767577.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNSMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            complement(15468..16091)
                     /gene="tesA"
                     /locus_tag="OO544_001970"
     CDS             complement(15468..16091)
                     /gene="tesA"
                     /locus_tag="OO544_001970"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HCR6767578.1"
                     /translation="MNFNNVFRWHLPFLFLALLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            16062..16748
                     /locus_tag="OO544_001971"
     CDS             16062..16748
                     /locus_tag="OO544_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6767579.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            16745..19159
                     /locus_tag="OO544_001972"
     CDS             16745..19159
                     /locus_tag="OO544_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6767580.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGNENQLDGYDKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLTLSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMQLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVAPLKAF
                     LAEHHIVPESFYPVVRARLTAINDKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFAG"
     gene            complement(19393..20487)
                     /gene="mnmH"
                     /locus_tag="OO544_001973"
     CDS             complement(19393..20487)
                     /gene="mnmH"
                     /locus_tag="OO544_001973"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HCR6767581.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMDAWRAACLQNPQGILCCARGGQ
                     RSHIVQSWLYAAGIDYPLVEGGYKALRQAAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTDSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKTQ"
     gene            complement(20556..21482)
                     /gene="allS"
                     /locus_tag="OO544_001974"
                     /pseudo
     CDS             complement(20556..21482)
                     /gene="allS"
                     /locus_tag="OO544_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
BASE COUNT         5015 a         5684 c         5799 g         5144 t
ORIGIN      
        1 gatcggcgat aagccccaga actgccgctc ccagacctcc catgccaaaa gcaaaaccga
       61 aaaagagtcc agaaaccata ccgatacgtc ccggaagtag ctcctgagcg tagaccagaa
      121 tggcagagaa tgccgaagcg aggataaatc caataatcac cgttaaaacc cccgtccagt
      181 gcaggctggc gtagggtaaa atcagcgtaa acggcgcaac gccgaggata gagccccaaa
      241 tcacatattt ccgtccaatt ttatccccta caggcccgcc gatcaccgta cctgccgcaa
      301 cggcaaacag gaaggcaaac agatgaagct gggcattctg gatagataat ccgaattttt
      361 gcatcagata aaaggtgtaa tagctgctga tgctcgccat atagaaatat ttcgagaaaa
      421 tgaggattaa cagaatgctg actgccagta ccactttgtt gcgcggcagt ggattgatta
      481 tcgtcgcttt gggttttcct ttattcattc ggtgctgtgc cgagtaccaa cggctaattt
      541 gcgccaacac cacgatcgcc agcagtgccg caagcacaaa ccaggcaacg ttgcctttgc
      601 cataaggcgc gataatcacc gccgccagca agggtcccag ggaactgcca aagttgccgc
      661 cgacctgaaa gatagattgc gccaggccat gccgcccgcc ggaagccata cgggctacgc
      721 gagaagattc cggatgaaag accgatgaac cggtaccgac cagcgccgcc gccagcagaa
      781 ctgcgccaaa actgcccgcc agcgcaagca gcaccagacc acttaaggta aagcacatgc
      841 caattggcaa cgaccacggc atcggatatt tatcggtcca gtagccgacc actggttgca
      901 gtagcgaaga ggcgagctgg aaggtgaggg ttatcatgcc aatctgcata aatgtcagag
      961 aaaattctga ctgaagcagc ggataaatcg ccagaatcag cgattggatc atgtcgttca
     1021 gcagatgtga gaggctgata gcacctaaaa taccaaacga tgttcgggcc ttggtcgttg
     1081 acgcagccgc gcccgccaca ggctggggtt gttcactcat tgccatagga aagtcacttt
     1141 ttcagggttg cgatgtaaag aatgatctta tttgtgatta ttacccgact aacatacctg
     1201 tatgcgtcgt ctgaaggaag tctcaacgcc gaatacagaa tttctaatct ggatgcagat
     1261 ttatcttcac cggacgcaga cttgtctatg atgtcgcgtc atactatttt tcaacacgtt
     1321 gaaatcaggt cagggagaga agtatgaaat tattgcagcg gggcgtggcg ttagcgctgt
     1381 taaccacatt tacactggcg agtgaaactg ctctggcgta tgagcaggat aaaacctaca
     1441 aaattacagt tctgcatacc aatgatcatc atgggcattt ttggcgcaat gaatatggcg
     1501 aatatggtct ggcggcgcaa aaaacgctgg tggatggtat ccgcaaagag gttgcggctg
     1561 aaggcggtag cgtgctgcta ctttccggtg gcgacattaa cactggcgtg cccgagtctg
     1621 acttacagga tgccgaacct gattttcgcg gtatgaatct ggtgggctat gacgcgatgg
     1681 cgatcggtaa tcatgaattt gataatccgc tcaccgtatt acgccagcag gaaaagtggg
     1741 ccaagttccc gttgctttcc gcgaatatct accagaaaag tactggcgag cgcctgttta
     1801 aaccgtgggc gctgtttaag cgtcaggatc tgaaaattgc cgttattggg ctgacaaccg
     1861 atgacacagc aaaaattggt aacccggaat acttcactga tatcgaattt cgtaagcccg
     1921 ccgatgaagc gaagctggtg attcaggagc tgcaacagac agaaaagcca gacattatta
     1981 tcgcggcgac ccatatgggg cattacgata atggtgagca cggctctaac gcaccgggcg
     2041 atgtggagat ggcacgcgcg ctgcctgccg gatcgctggc gatgatcgtc ggtggtcact
     2101 cgcaagatcc ggtctgcatg gcggcagaaa acaaaaaaca ggtcgattac gtgccgggta
     2161 cgccatgcaa accagatcaa caaaacggca tctggattgt gcaggcgcat gagtggggca
     2221 aatacgtggg acgggctgat tttgagtttc gtaatggcga aatgaaaatg gttaactacc
     2281 agctgattcc ggtgaacctg aagaagaaag tgaccaggga agacgggaaa agcgagcgcg
     2341 tgctgtacac ccctgaaatc gctgaaaacc agcaaatgat ctcgctgtta tcgccgttcc
     2401 agaacaaagg caaagcgcag ttggaagtga aaataggcga aaccaatggt cgtctggaag
     2461 gcgatcgtga caaagtgcgt tttgtacaga ccaatatggg gcggttgatt ctggcagccc
     2521 aaatggatcg cactggtgcc gactttgcgg tgatgagtgg aggaggaatt cgtgattcta
     2581 tcgaagcagg cgatatcagc tataaaaacg tactgaaagt gcagccattc ggcaatgtgg
     2641 tggtgtatgc cgacatgatc ggtaaagagg tgattgatta cctgaccgcc gtcgcacaaa
     2701 tgaagccaga ttcaggtgcc tacccgcaat ttgccaacgt tagctttgtg gcgaaagacg
     2761 gcaaactcaa cgacctgaaa atcaaaggcg aaccggtcga tccggcgaaa acttaccgta
     2821 tggcgacatt aaacttcaat gccaccggcg gtgatggcta tccgcgcctt gataacaaac
     2881 cgggctatgt gaataccggc tttattgatg ccgaagtgct gaaagcgtat atccagaaaa
     2941 gctcgccgct ggatgtgagt gtttatgaac cgaaaggtga ggtgagctgg cagtaatccg
     3001 aaagtgccgg atatttgcat ccggcacaat gcttaatcgc ggcgggcgat atcagcaaat
     3061 ttggcatcga ggatcttcgc cagatcgcct gccgccagtt cgatatccag tccgcgcttg
     3121 ccgccagaaa cataaatagt ggcaaattct tgtgcggggg cgtcgataat cgtcggcaga
     3181 cgttttttct gccccaatgg gctaatcccc ccaaccaggt atcccgtcga acgctgcgcg
     3241 gccatcggat cggccatctc aactttcttg gcacccagcg cttttgctac ttttttaaga
     3301 tccagttgac ctgcgaccgg cgtaacggcc actgccagat gtttcatatc accgttcact
     3361 gccaccagta gcgttttgta gacttgatcc ggattcaaac ctaatttttt gacgacttca
     3421 tcgccaaaat tggtttcagc cggatcgtgc tcgtaggtat ggatttgaaa cgaaatcttg
     3481 tttttttcga gtaatttaac tgcgggtgtc atggtaattt tcctgcctta aacaaaaaaa
     3541 cgaagcaagc atacgcctga tggcggtcca aaaaaaagag tcatcttgcc taagagtatt
     3601 ggcaggatgg tgagattgag cgacaatcga gttacaccgt cgcttaaagt gacggcataa
     3661 taataaaaaa atgaaattcc tctttgacgg gccaatagcg atattggcca tttttttagc
     3721 gcaacatttg cggcaaattc ccttctccat acaggtgtag tgcaccgacc gcgaccacat
     3781 atcgccccgg cggcatggcg cgtaatttat cccgccaggc gagatttcgc tgatgcatca
     3841 gcacatcgta cagcgactga ctgaacgtat tgggcagcgt tatatcatta ttttgcggag
     3901 gtgcattcag ccaccagctc atcatttgtt gcagcaaccg tgcgttggta tgccagtggg
     3961 tcagcgtatc gtccagcagc gccagtcctt tgtcagggag ctggagcaac atggtaatct
     4021 ggttttcagc cccttccagt tcaatcacgg gtttatgttg ttgcttcgcc gcctgcaata
     4081 actggtaatc gataccgtat tctgcccgca gccccagttt ttgtgcctgc gtcgcctgaa
     4141 gaaccatcgc gatttgccac agcggttgag tagaaaagag cgacggagaa atgcccatct
     4201 cctggctaat gtgctgcaga ttttggagtt gctcctcgtt aatgcgctct tccagcgcct
     4261 cgcaagtagg caaattagca aaaggcgtat cgctggtgga aacatccgcc tcgacgatca
     4321 gcgcatcggc gtttttgagc tttttgagca aacgggtggg caggggagcc atatcgtggc
     4381 tacccatatg aatactgcca atcagatgaa aatggcgatt gccgggaagg gtgatatcga
     4441 tggcaggcca ggtgtaatga ttaccgcgca gcgcggccca aagcgttttt acccggtaca
     4501 acagatccat acgacctccc tttgtgaaat atcatgctag cgcgcggtga gggatggcgc
     4561 aaatggagtt ggggacttgt ttaatgtgtt tgtatgattc agtatgttct tgcatcgcta
     4621 ttcacaggga agcaacagtt aaaaaccatg aaacaggcaa caagaaaacc gacgacaccc
     4681 ggagatattc ttctctatga atatctggaa ccgctcgatt tgaaaatcaa tgagttagca
     4741 gagttgctgc atgttcatcg taatagcgtc agtgcactga tcaataacaa tcgtaaactc
     4801 accactgaga tggcattccg tctggcgaaa gtttttgata ccacagtcga ttttaggcta
     4861 aacctccagg cggcagttga tctttgggaa gttgaaaaca acatgcgcac ccaggaagaa
     4921 ttgggacgga ttgaaacagt ggctgaatat ttggcacgcc gtgaagagcg tgcaaaaaag
     4981 gtcgcgtaag gccaaaacat cgcttcatct cgtaggccgg ataaggcgtt cacgccgcat
     5041 ccggcatccg agcaccattg cctgatgcga cgcttacccg tcttatcagg cctacaaacc
     5101 tgtgcctgaa ccgtaggccg gataaggcgt ttacgccgca tccggcagtc atgcgtcgat
     5161 gccaaatgcg ccgccctaaa gcagcgcatc cgcaatgacg tacttattcc ttcggtttaa
     5221 accgcagcaa ccggttggcg ttactcacta cggtaatcga cgagagcgcc attgccgctc
     5281 cggcaactac cgggttaagc agtgttcccg taaacggcca caaaataccg gcggcgaccg
     5341 gaataccgat gctgttgtag ataaaggcac cgagcaggtt ctgcttcatg ttgcgcagcg
     5401 ttgcgcggga aatcgcgagc gcatccgcaa cgcccatcag gctatggcgc atcagggtaa
     5461 tcgccgcggt ttcaatggca acatcactgc cgccacccat cgcaatgccg acatccgcct
     5521 gggccagcgc tggcgcgtcg ttaatgccgt cgcccaccat tgccacctga cgtccttcac
     5581 tttgcagacg tttgatcgct tcggctttac cgtccggcag cactccggca atcacttcat
     5641 caattccagc ttctttggcg atcgcattgg cggtggttgg gttatccccg gtcaacatca
     5701 ccagacgata tcccgtttta tgcaggcgtt gcagcgccgc cacgctatca ctacgcaacg
     5761 gatcgcgtac agccagcagg gctactgctt tcccgtcaat cgccagcagc acaggcgttg
     5821 ctccttgcga tgcctgagca gtaatctccg cttcgatagc tttggtacca acctgttgct
     5881 catttaacag cgcctgattg cccagcaata acgcatgacc ttcagcttca ccgctcacgc
     5941 ccagcccgcg caatgtgcgg aaaccgttga cctgcggcag ctgcatatca cctgctttat
     6001 cgaggatcgc ccgtgccagc ggatggctgg aaccttgctc cagtgccgcc gccagacgca
     6061 atgcctgcgc ttcatcaaca tcagcaaacg ttttcactgc gacaacctgc ggcttccctt
     6121 cggtcagcgt cccggtttta tcgaacacga tggtatcgag tgtactggcg cgttgcagcg
     6181 cgtcggcgtc ccgcaccagc acgccaaact cagccgcccg cccgacgccg gaaataatcg
     6241 acatcggcgt cgccagcccc agcgcacacg gacaggcgat aatcagtacc gtggtggcaa
     6301 tcaccagggt ataaacaatt tgcggtgccg gaccaaagaa gtaccagatt gccgcactga
     6361 ccagcgcaat caccaccacc acaggtacaa atacggctga gattttatcc gccagctgac
     6421 cgatttctgg cttgctgctc tgggcctggc gcaccatgcg aatgatccgt gacagcgtgg
     6481 tatggctgcc aaccgcactg gcacgaaaca gcacactgcc gtcctgtacc actgtcccgg
     6541 catggacgct ttcgccttcg cctttttgct gtgggattgg ttcgcccgtc agcatcgctt
     6601 catccagcca tgcttcgccc tgggtaatct cgccatctac cggcacgcga tcgccggtcg
     6661 tcaggcgcag taacatccct ggctgaactt ccgccagcgg cacgcttttt tcaccatctg
     6721 cggtgaccac tcgcgccgtc ggtggggtta aatcgagtaa tttctccagc gccttagaag
     6781 aacgctgacg tgcgcgcgct tccagcatat ggccgagatt aatcagaccg ataatcatcg
     6841 cactggcttc atagtaaaga tgccgcgctt ccatcgggaa ccactgcggc cacaggttga
     6901 cgctcatcga atagagccac gccacacccg ttcccagcgc caccagcgta tccatcgtcg
     6961 ctgcaccgtt cagcaggctt ttccatgcac tgcggtaaaa atggccgccg gcgaaaacca
     7021 tcactgccag ggttatcagg ccgataacta accacaagct gcggttgtca gcggtgacca
     7081 tcatgttatc gccgatcatc ccccagacca ttaccgggat acccacagcc agtgcaacaa
     7141 ttgcctgcca gcggaagcgc ttcatcgtag ctacggcggt ttcttgctgg cgctcgcggc
     7201 gtttagcgtc atcttcaatc gcttccgcac cgtagcccgc tttttccacc gcctgcacta
     7261 aatcttgtgg ggaggcactg cccatcacca gcgcagtacg ctccgccagg tttacccgtg
     7321 cctgagtgac gcccggtacg ctttgcagcg cattttgtac gcgggtgaca cagctggcgc
     7381 agctcatgcc gctcagcagc aactgctggc tgtcatcgtc atcggcggtc gctgccggaa
     7441 gcgcctcaga aaccgctgtc agtgcttccg acgggattga tgactccgcc agcggtttag
     7501 cctttgggtg gcttacagat gcgtcataac ccgcttgttt gatggtttcg atcagctgtt
     7561 ctgcactggc agtcccggta acgtgcgctt cagtgataga cacatccgcc tgctcaacat
     7621 ccgggcgctg ttcaagactt tctttcacgc gtttaacgca gtgaccgcag gacaggccgt
     7681 ccagggtcag gtcgatagtt tgtgacataa aacactcctt taagacagtt ttgactggct
     7741 gtgataaagg ttaaaccttc cagcaagggg aaggtcaaga aattaataaa ccaggcgggt
     7801 aaaagtccgt aaagattaaa aaatcggctc gatttgcatc aggattagac atttatttct
     7861 ttgttttcct gtagttaagt tgcgggtgct aagttaaatc aggatgcctg aaaatcggca
     7921 ccggggtgag gaattacctc ccgcatctat aaaaaggagt taacaaaaga tgttagatgc
     7981 aaacaaatta cagcaggcag tggatcaggc ttacacccaa tttcactcac ttaacggcgg
     8041 acaaaatgcc gattacattc cctttctggc gaatgtacca ggtcaactgg cggcagtggc
     8101 tatcgtgacc tgcgatggca acgtctatag tgcgggtgac agtgattacc gctttgcact
     8161 ggaatccatc tctaaagtct gtacgttagc ccttgcgtta gaagatgtcg gcccgcaggc
     8221 ggtacaggac aaaattggcg ctgacccgac cggattgccc tttaactcag ttatcgcctt
     8281 agagttgcat ggcggcaaac cgctgtcgcc actggtaaat gctggcgcta ttgccaccac
     8341 cagcctgatt aacgctgaaa atgttgaaca acgctggcag cgaattttac atatccaaca
     8401 gcaactggct ggtgagcagg tagcgctctc tgacgaagtc aaccagtcgg aacaaacaac
     8461 caacttccat aaccgggcca ttgcctggct gctgtactcc gccggatatc tctattgtga
     8521 tgcaatggaa gcctgtgacg tgtacacccg tcagtgctct acgctcatca atactgttga
     8581 actggcaacg cttggcgcga cgctggcggc gggtggtgtg aatccgttga cgcataaacg
     8641 cgttcttcag gccgacaacg tgccgtacat tctggccgaa atgatgatgg aagggctgta
     8701 tggtcgctcc ggtgactggg cgtatcgcgt tggtttaccg ggcaaaagcg gtgtaggtgg
     8761 cggtattctg gcggtcgtcc ctggcgtgat gggaattgcc gcgttctcac caccgctgga
     8821 cgaagaaggc aacagtgttc gcggtcaaaa aatggtggca tcggtcgcta agcaactcgg
     8881 ctataacgtg tttaagggct gatcatgatg aacacggaag gtaataacgg taacaaaccc
     8941 ctcggtctat ggaacgtcgt ttccatcggt attggggcaa tggtgggggc ggggatcttc
     9001 gcgctgctgg ggcaggctgc gttgctaatg gaagcctcga cctgggtcgc ctttgctttt
     9061 ggcggtattg tggcgatgtt ttccggttat gcctatgcgc gtctgggggc gagctatccc
     9121 agcaatggcg gtattatcga cttttttcgt cgcggattag gcaacggcgt cttttcgcta
     9181 gcgctctcgt tgctgtacct gttgacgctg gcggtgagca tcgccatggt tgcccgtgct
     9241 tttggcgctt atgccgtgca gtttttgcat gaaggcagcc aggaggagca ccttattttg
     9301 ctctacgcgt tggggatcat tgcggtgatg acgcttttta actccttaag caaccatgcg
     9361 gtagggcggc tggaagtgat cctcgtcggc attaaaatga tgatcctgtt attgctgatt
     9421 attgccggtg tctggtcgct gcaaccggcg catatttccg tctctgcgcc ccccagctcc
     9481 ggtgcgttct tctcctgtat tgggataacc ttccttgcct atgcgggctt tggcatgatg
     9541 gcgaacgcgg cggataaagt gaaagatccg caggtcatta tgccacgggc gtttctggtg
     9601 gcgattggcg ttaccacgtt gctttatatc tcgctggcgc tggttttgct tagcgatgta
     9661 tcggcattag agttagaaaa atatgctgat accgccgtag cgcaggctgc ttctccgctg
     9721 ctcgggcatg tgggttatgt gatcgtcgtc atcggcgctt tactggcgac ggcttcagcc
     9781 attaacgcga acctgttcgc cgtgtttaac atcatggaca acatgggcag cgaacgcgaa
     9841 ctgccgaagc taatgaataa atccctgtgg cggcagagta cctggggcaa cattattgtc
     9901 gtggtgttga ttatgctgat gacggcggcg ctaaatttag gctcactcgc cagtgttgcc
     9961 agcgccacct ttttgatttg ctacctggcg gtgtttgtgg tggcgatccg cctgcgtcat
    10021 gatattcacg cctcgttgcc gattcttatc gttggtacgt tggtgatgtt gttggtgatc
    10081 gttggcttta tctacagtct gtggtcccag ggtagccgtg cgttgatatg gattattggc
    10141 tcactcttac tcagccttat tgtggcaatg gtcatgaagc gcaataaaac cgtataacat
    10201 ctctctgtgc gcagtacttc ctgtattatt gtggtggcgg tcgatattcg cactggcaaa
    10261 aaaacgtgct tgaatatctg ttgaaaccct ttaacaaagc acaggaggcg ttgcgcgaac
    10321 gatgaacatc agcgatgtag caaaaattac cggcctgacc agcaaagcca ttcgcttcta
    10381 tgaagagaag gggctggtga cgccgccgat gcgcagcgaa aacggttatc gcacctacac
    10441 gcagcagcat ctcaacgaac tgaccttact gcgccaggca cggcaggtgg gctttaacct
    10501 ggaagagagc ggcgagctgg tgaatctgtt taacgacccg cagcggcaca gcgccgacgt
    10561 caaacggcgc acgctggaga aggtggcgga gatcgaacga cacattgagg agctgcaatc
    10621 catgcgcgac cagctgctgg cactggcgaa tgcctgccct ggcgatgaca gcgccgactg
    10681 cccgattatc gaaaatctct ccggctgctg tcatcatcgg gcagggtgat taagacgaga
    10741 cggctcggat atgtagggtt atcccttcta tcgcaatgac ttcaacatgc gtacctgcac
    10801 cgagatcctc gctggcgctg acaggccatg aactgtcacc gacgcgcata tgaccgcgcc
    10861 cgttgaccag cggagattcc agcacaaaac gtcggccaat cagctgctgc ccgcgctggt
    10921 ttaaatgact atcgctgtgc ttttgttcgc gcacccgccg cgacaaccat ttccaccaca
    10981 gccaggcggc gagcagcgtc aggatggcaa acattacccc ttgccactcc caacccagcg
    11041 gcaccagcca gaccaccagg ccggtaatca ccgctgccac gccgctccac aacaaataac
    11101 catttccgcc cagcatctcg gctgccagca gcaaaccgcc gagactgagc cagaaaatat
    11161 gtggatgaac gaccattaac tccatcatgg ctgagtccgt ttgttggcgc tgtctttcac
    11221 cagctcggca atcccggcaa tcgaccccat caggctgctg gcctctaatg gcatcatcac
    11281 tactttgctg ttactggagg taccgatctg ctgtaacgct tcggtgtatt tctgcgctac
    11341 gaagtagttc accgcctgaa tatcaccgga ggcgatagct tcagacacca ttttggtggc
    11401 gcgggcttct gcttcggcgg aacgttcacg cgcttcagcc tgtaaaaacg ccgactgacg
    11461 ttcgccttcc gctttcagga tttgcgactg tttttcacct tcggctttga ggatttccgc
    11521 ctgacggatc ccttccgctt caagaatgta agcgcgtttg gtacgttccg ctttcatctg
    11581 cgcgttcatt gaagagataa gctctgccgg tgggcgcacg tcgcgaattt caatacgggt
    11641 gactttaatc ccccacgggt tggtggcttc atcgacaata cgcagcaggc gtgagttgat
    11701 gctgtcgcgc tgagagagca tttcgtcaag ttccattgaa cccaacacgg tacggatgtt
    11761 agtcatggtc aggttgatga tcgccagctc cagattgctg acttcataag ccgcgcgcgg
    11821 cgcgtcaatc acctgaataa aacagactgc gtcgatggta acgttggcgt tatctttcga
    11881 gataacttcc tgggaaggga tatcgagcac ttgctccatc atattgatct tgcgaccaat
    11941 gcgatccata aacggcacca ccagactgag ccccggctgt aacgttttgg tatagcgacc
    12001 aaaacgttct accgtccatt gatagccctg cggcacgatt ttgacgcccg cgccgacaat
    12061 gaccagcgcg acaaaaatga gaatcgggat aaagataagc atcggaaaaa cctcctgttg
    12121 taccgtccat aatcagcaaa attgctgctt gattaaacaa attatacctg attactgaaa
    12181 gagagttccc ccttattcct gcgaaggata aactgttttt agtaaaaatc agaaaaaggg
    12241 aacagcgatg caggaaaata gtcctttgct tcagctacaa aacgtaggat atctggcggg
    12301 tgatgcgaag attcttaata acatcaattt ttcgctgcgt gctggcgaat ttaagttaat
    12361 taccggtcct tctggttgtg gcaaaagtac gctgctaaaa atagttgctt cattgatcag
    12421 cccaaccagc ggaacgttac tgtttgaagg tgaggatgtc agcacactaa agccagaaat
    12481 ctaccgccaa caagtctctt actgcgccca gacaccgacg ctgtttggcg atacggtata
    12541 cgataatctg atctttccct ggcagatccg taaccggcag cctgacccag ccatttttct
    12601 cgattttctc gaacgcttcg ccttgccgga cagcattttg acgaaaaata tcgccgagct
    12661 atctggtggt gaaaaacaac gcatctcact gattcgtaac ctgcaattta tgccgaaggt
    12721 tttattgctg gatgaaatca ccagtgcgct ggatgaaagt aataaacata acgttaatga
    12781 gatgatccat cgttatgtgc gcgagcaaaa tattgccgtg ctgtgggtga cacacgataa
    12841 agacgaaatt aatcatgcgg ataaagtgat tacactgcaa ccgcatgccg gagaaatgca
    12901 ggaagcacgc tatgaactcg cataatatta ctaacgaatc attagcactg gcattaatgc
    12961 tggtggtggt ggcaatctta attagccata aagaaaaact ggcgctggag aaagatattc
    13021 tctggagcgt cggacgagcg ataattcagc tgattattgt cggctatgtg ctgaagtata
    13081 ttttcagcgt ggatgatgcc agcctgacat tattgatggt gttatttatc tgctttaatg
    13141 cggcgtggaa cgcgcaaaaa cgcagtaaat atattgctaa agcttttatc tcatcgttta
    13201 ttgccattac ggtcggggcg ggaattaccc tggcggtgct gattctctca gggtcgattg
    13261 aatttatccc gatgcaggtg atccctatcg ccgggatgat tgccggtaac gccatggtag
    13321 cggtggggtt gtgttacaac aatttagggc aacgggtcat tagcgaacag caacagattc
    13381 aggagaaact gagtcttggt gcgacgccga agcaggcttc agcgatattg attcgcgaca
    13441 gtattcgcgc ggctttaatt ccgacggtcg attcagcaaa aacggttggc ttagtgagtt
    13501 taccaggaat gatgtccggg ctgatatttg ccgggattga tccggtgaag gcgattaaat
    13561 atcagattat ggtgaccttt atgctgctct caaccgccag cttgtcgacc attattgcct
    13621 gctatttaac ctatcgtaag ttttataatt cgcgccacca gttggtggtg acgcaattga
    13681 agaagaaatg atgcgatgcc ggatgcgcag catcgcatcc ggcgttgtgg ttcatgtgcc
    13741 gatcaacgga atatcaatac aacaatgcat acagctggcg gcgatacttc gacgccagtg
    13801 catcacccgt acccagcgca gcgaggatct cctggaacgt tttacgcgtc tgaccgtctg
    13861 cggcggtgag atctttacgc agatgcccga acagcaactc cagcgcctct tcattgcgcc
    13921 caacctgatg cagttgcagc gccagttgcg tcgccagtgc ggcatcttct ggattctccg
    13981 ccacctgctg ttgcaactgt tgaatttccg gcgtatcagc cgcctgcttc agcagttcga
    14041 tttgcgccac catcccctgg tagcgggtgt cctgatcctg taacggaatg gttttcagca
    14101 ccgcttctgc atcttcagaa cggttcagcg caatcagcgt ttctgccaac agcaggccga
    14161 tctccccgtt ctgattcgac aactgccagg cgtctttcag caatggcagg gcatcggtga
    14221 aattgccttc ctgcatcagt tgcatcgcct gctgcgcttt cagctcttct tcgcgcggca
    14281 gcactttatc cagcagggcg cggatcgcct cttccggttg cggcccctgg aagccatcta
    14341 ccggttgccc gttctggaac agatacaccg tcggaatcgc gcgcagacca aactgggcgg
    14401 caatcatctg ctccgcatcg cagtccagct tcgccagaat aaattgcccg ttgtactgcg
    14461 ccgcaaggct ttccagaatt ggggttaact gcaaacagtg ctggctacgt tcagaccaaa
    14521 aatagaacag caccggagtg gtcatcgact gttcaagaac ctgttgcagg ttagattcgt
    14581 taatgttgac aatattttct acggacatgg agtcgctctc tgttgtcgat attttctttg
    14641 acatgggggc ttaagcgcgc gcttcaactc acccctgcaa tattttgtcc atcacgcgcc
    14701 ccggcagcag gcgcttaagc accattactg cccaagtcac cagcgttacc ggatagcgca
    14761 tcttcggctt ctcgctaata aaagcatggc gtactttgtc caccaccgct tccggtccca
    14821 acgtaaagcg ggcggcgatg ccgggatttt cgactggttt atcactttgc gtctggttga
    14881 cgttgtcggt gaagcgagta cgaatgggac cgggttcgat caggctgact ttaattccgc
    14941 tgtggcgtag ctccatgcgc agcgcgtctg accatgcctc cagcgcatat ttactggccg
    15001 cgtaagcgcc acgacccggt gtggagatta atcccatcac cgatgatgtc atcacaatac
    15061 gcccttcacc gtgcggtaac atcgcgggta acaggcgcat ggtgagctgg tgtgcgccga
    15121 aaaagttggc ggaaaactgc tgttccatct gcgcgcggct gatggtggaa agggggccat
    15181 acatgccgaa tccggcattg ttaaagatcc catacagaca attatcggtc agggcgatca
    15241 cttcgtcggc tgcgcgatca acactttctg gcgaatccag atcgatcaac acgccggtaa
    15301 atcccatgct gttcatacgc tcaacatcat ccggtttccg gcaacctgcc agcacatgaa
    15361 aaccctggcg ttttaattcg agcgcgcttt ccaggccaat tccactggaa catccggtaa
    15421 ttaagaccga tttttgcata actttacctg tcaggatctc cgttgcttta tgagtcatga
    15481 tttactaaag gctgcaactg cttcgccatc cagtcggcaa taaacggctg ggcgtcgcgg
    15541 ttgggatgaa taccgtcatc ctgcatccat tgtggcttga ggtagacctc ttccataaaa
    15601 aagggcagca gcggaacatc aaactctttg gcgagtttgg ggtaaatggc gctaaaggct
    15661 tcattataac ggcgaccata gtttgcaggc agacgtattt gcattaacaa tggttcagcg
    15721 ttggcggctt tgacatcctg caaaatctgg cgcagcgttt gctcggtttg ctgtggctga
    15781 aaaccacgca aaccgtcatt gccgcccagt tcaaccagca cccaacgcgg ctgatgctgt
    15841 ttcagcagag ccggaaggcg cgccagtcct tgttgcgagg tgtcgccgct gatgctggca
    15901 ttaactaccg acgttttact ctgccactta tcattcaaca aggcaggcca ggccgcgctg
    15961 gcagacattc gatacccggc gctcaggcta tcacccagaa tcaataacgt gtccgctgcg
    16021 gcggcacgga aggttaacag ggccaggaac aggaagggca aatgccagcg gaaaacattg
    16081 ttgaagttca tcatcttaag aagtccgtcg gtcaggggga gcatgaactc tccatcctca
    16141 ccggagttga gctggttgtc aaacgtggcg agaccatcgc actggtgggc gagtcgggat
    16201 cgggtaagtc aaccttgctg gcgatcctcg ccgggcttga tgacggcagc agtggcgaag
    16261 tgagtctggt gggacaaccg ctacataata tggacgaaga agcgcgggca aagttgcgcg
    16321 cgaagcacgt cggctttgtt tttcagtcat ttatgttaat tcctaccctt aacgcgctgg
    16381 aaaacgtcga gcttccggct ctgctgcgcg gtgagagtag cgcggaaagt cgtaacgggg
    16441 cgaaagcgtt gctcgaacag ttagggctgg gtaaacgtct ggatcatctt ccggcacagc
    16501 tttccggcgg tgaacagcaa cgagtggcgc tggcacgagc ctttaatggt cgacctgatg
    16561 tgctgtttgc cgacgaaccc accggcaacc ttgaccgcca gacgggcgat aaaattgccg
    16621 acctgctgtt ttccctcaac cgtgaacatg gcaccacgtt gattatggtg acccacgacc
    16681 tgcaactggc ggcacgctgc gaccgctgct tacggctggt gaacgggcag ttgcaggagg
    16741 aagcatgatt gcacgttggt tctggcgcga atggcgttcg ccgtcgctat taattgtctg
    16801 gctggcgcta agcctggcgg tggcctgcgt gctggcgctg ggcaatatca gcgatcgcat
    16861 ggagaagggc ttaagccagc aaagccgtga gtttatggcg ggcgatcggg cgttgcgcag
    16921 ttcacgcgaa gtgccgcaag cgtggctgga ggaagcgcaa aagcgcggcc tgaaagtcgg
    16981 caagcagctg actttcgcca caatgacctt tgcaggcgac acaccgcagc tggcgaacgt
    17041 caaagcggtg gatgatatct acccgatgta tggcgatctg caaactaatc cccctggcct
    17101 gaaaccgcag gcgggcagcg tattgctggc cccacgcctg atggcactgc ttaacctgaa
    17161 aacgggcgac accattgacg tgggcgatgc caccttgcgg attgccggag aagtgattca
    17221 ggaaccggat tccggtttta accccttcca gatggctccg cgtctgatga tgaatctggc
    17281 ggatgtcgat aaaaccggag ccgtgcagcc ggggagtcgg gtcacctggc gttataaatt
    17341 cggcggcaac gagaaccagc tcgacggcta tgataaatgg ttgttacctc agcttaaacc
    17401 cgaacaacgc tggtacggtc tggaacagga cgaaggcgcg ctggggcgat cgatggaacg
    17461 ctcgcaacag ttcctgctgc tttcggcgct tctgaccttg ctgctggcag tggcagcggt
    17521 ggcggtagcg atgaatcatt actgtcgcag tcgctacgat ctggtggcga tcctcaaaac
    17581 gctgggggca gggcgagcgc aactgcgtaa gctaatcgtc ggtcagtggt tgatggtgct
    17641 gacgctttca gccgttaccg gtggggccat aggcctgttg ttcgaaaacg tgttgatggt
    17701 gctgctcaag cccgttctgc ctgctgcact accgccagcc agcctctggc cgtggctgtg
    17761 ggcgcttggc accatgacgg tcatctcgct gctggtgggg ctacgaccat accgcttgtt
    17821 gctggcaacg cagcctttac gcgtattacg taatgatgtg gtagcgaacg tctggccgct
    17881 gaagttttat ctgccgattg tcagtgtggt ggttgtgctg ctgctcgccg gattaatggg
    17941 tggcagcatg ctgctttggg cggtgctggc gggcgcggta gtactggctt tgctgtgcgg
    18001 tgtgctgggc tggatgcagc tgaatgtact tcgccgcatg acgctgaaat cgctacctct
    18061 gcgcctggcg gttagccgcc tgttacgtca gccgtggtca acgttaagcc agctttcggc
    18121 attttcgctc tcctttatgc tgctggcgct gttgctggta ttgcgtggcg atctgctcga
    18181 ccgctggcag cagcagctac cgccagaaag cccgaactac tttttaatta acatcgccac
    18241 agaacaggta gcaccgctaa aagcgttcct cgcggaacat catatagtcc cggaatcatt
    18301 ttatccggta gtgcgggcgc ggctgacggc gattaacgat aagccgacag aaggtaatga
    18361 agatgaggcg cttaatcgcg aactcaatct tacctggcaa aatacgcggc ccgatcataa
    18421 tccgattgtc gccggtaact ggccgccaaa agccgatgaa gtgtcgatgg aagaggggct
    18481 ggcgaaacgc ttaaacgttg ccctcggtga taccgtgact tttatgggcg atacccagga
    18541 gttccgcgct aaagtgacca gcctgcgcaa agtggactgg gaaagtctgc ggcctaattt
    18601 ctattttatt ttcccggaag gggcattaga cgggcaaccg cagagctggc ttaccagttt
    18661 ccgctgggag aatggcaacg gcatgttgac gcaactcaac cgccagttcc cgaccattag
    18721 cctgttagat attggcgcga ttttaaaaca ggtcggtcag gtgctggagc aggtaagtcg
    18781 ggcgctggag gtgatggtgg tgctggtcac cgcctgcggt atgttgctgt tgctggcaca
    18841 ggtgcaggtg ggaatgcgtc agcgtcatca ggagctggtg gtgtggcgca cactcggtgc
    18901 ggggaaaaaa ctgctgcgta ccacgttgtg gtgtgagttc gccatgcttg ggtttgtttc
    18961 tggcctggta gccgcaattg gtgcggaaac ggcgctggca gtgttgcagg cgaaagtgtt
    19021 tgatttcccg tgggagccag actggcgatt gtggattgtt ctgccgtgca gcggtgcact
    19081 gctgctgtcg cttttcggcg gctggctggg tgcgcgactg gttaagggta aggcgctgtt
    19141 caggcagttt gcggggtgat gacaggtgtt agattgcctg atgcgctacg cttatcaggc
    19201 ctacgaagag cgtgcaatat attgattttg caagattgtg taggccggat aaggcgttta
    19261 cgccgcatct ggcatgaaca gtgtgcattt tggcaataaa tatgaaacca actgtcatgt
    19321 cggtttttta tatctgtatt ctggtgcaac cattctgttc tcacgacaat cgccgtttca
    19381 gaataccgcc catcactgcg tcttaaccca ttccgccact tccgcccact caccgcgaaa
    19441 gacaactttt tccgcttttt tctcaagctg atagcgatac atcgggtcgt aatattcttt
    19501 aagtaacggc accagccagg ccagatgacc gtcggtgctg tcggtggtga gttgcgttgt
    19561 cagcgccgta tccagctggg cagcgagttc gttatagcgc tgtaacccca gccgacgctt
    19621 aatagccgaa agtccgtgat gcaggtattc gcaatattcc tgccagccct gttcgtcgcc
    19681 gtacgcgtgg gtaaaatcat gatgcatacg caagaaatac tcttcgttca ggcgctcaag
    19741 acggatctca aacggatctt ctaccaccgc aatcgccgcc tgagtcattc gctcgcgcag
    19801 gcattccggc aggtgattcg aaccgatcat ccggctttcg tcttccagca cccacaggcg
    19861 caaatcctga cgggcgtcgg tttttagcat ttcggcagcc agcaggtttt caaaactcgc
    19921 ctggctaagt tgtggttgta aggtgcgacc aaacgccgaa ccgcgatgac gcgctaaccc
    19981 ttccagatca acaccgttcg gctgttgctg cactaacagc gttttaccgc tgccggtaca
    20041 accgccaatc agcactatcg gtttttgtgc cagttcaata gtcgcctgaa tcgcggcttg
    20101 gcgcagtgcc ttataaccgc cttccaccag cggataatca atccccgctg catacaacca
    20161 gctttgcaca atatgtgagc gctgaccgcc acgggcgcag cagagaatac cttgcggatt
    20221 ttgcaggcac gctgcccgcc aggcgtccat gcgctgctga cgaatttcac ccgccaccag
    20281 tttatgcccc agcgccagcg ccgcgtctga gccttgctgt ttataacaag tgccaacggc
    20341 ggcgcgttca tcgttattca ttaacggcag gttgatagcg gcgggcattg cgccttgctc
    20401 aaactcgata ggggcgcgaa catcaataat gggcgtatca gcaatcagca gggcacgata
    20461 gtcctgttcc gtgtgtctct cttgcatagt taaaagtgaa cctcaaatca gcttgcgcgc
    20521 tattttacgc gccaacgcgc aaggaaactt gatttttaac tgcgtgggtt gccgaaaatt
    20581 tctaaaaatc cgctgatttc cggcctgcgc tgggtaaaca aggtcacaat atcttctacc
    20641 gctttgccgc tgccaaattt gcgccatgcc agactcaatg gcgaaggagg gcgcatcgtt
    20701 gggattaccc ggctgaccag ttgttgatta tcgatcattg actggcaaag tgattttggc
    20761 aaaaaaccaa tgccaacacc cgccagatgg gcggcgattt tcgtttccat atcgggaaca
    20821 ataatctctt tttgccccgg caatcgccag gcgacgcgtt tggttaaggt tcgggcgctg
    20881 tcttcaatat tgaccgccgg aaagcgccgc aactgcgctt ctgttagcgg ctcttcaacg
    20941 ttcgccagcg gatgatccgc cgccatgaca aagcgccatt gcaccgatcc taagggatca
    21001 agactaaagg tatttgccag cgcctcagtt cccgtgaccc cgatagccag cgaaaaacct
    21061 tcgtacaata gcgagtccca gacgcccata tagatttgtc gggagatgtg aaactgggta
    21121 aaggggtaac gctcattcag ccacgccagc aactgggcga cggcctgggg gttatagagc
    21181 aggttgttga tgacaatatt cacctagcgt tccacgccat cattcacctg ttgcagctcg
    21241 ctgggcatac tttccagcca gctcagccag tctctggcct gggaaagtag atgctcgcca
    21301 gccgctgtca acgtcacgct gcgagtcgtg cggaaaaaca gcgccactcc ggtattctct
    21361 tccagaagtt taatgcgata actgatcgtc gccgtggttt tacataatcg ttctgccgct
    21421 tttgaaaaac ttcctgtttc agcaaccgca atgaaagtcc gcaaggtttc tggatcgaac
    21481 atcttcaggt atcccctttt aaatccgcaa gttgcgtgat tttcttatcc tctgatttat
    21541 cagtattttt acatgataac cctgttcaat ttgtggacta aatctagttt tggaaaaata
    21601 ttccaacttt tgtattgatg ttgttctctt aaggttttag at
//
LOCUS       DAJTZL010000061        21555 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.061, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000061 DAJTZL010000000
VERSION     DAJTZL010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21555
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.061"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..1367
                     /gene="espL1"
                     /locus_tag="OO544_001975"
                     /pseudo
     CDS             1..1367
                     /gene="espL1"
                     /locus_tag="OO544_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
     gene            1539..1646
                     /locus_tag="OO544_001976"
     CDS             1539..1646
                     /locus_tag="OO544_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6767582.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(1699..2457)
                     /locus_tag="OO544_001977"
     CDS             complement(1699..2457)
                     /locus_tag="OO544_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HCR6767583.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            2744..3673
                     /gene="pfkB"
                     /locus_tag="OO544_001978"
     CDS             2744..3673
                     /gene="pfkB"
                     /locus_tag="OO544_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416237.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HCR6767584.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISASQKQG
                     IRCIVDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            3774..4064
                     /gene="ghoS"
                     /locus_tag="OO544_001979"
     CDS             3774..4064
                     /gene="ghoS"
                     /locus_tag="OO544_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HCR6767585.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            4170..5030
                     /locus_tag="OO544_001980"
     CDS             4170..5030
                     /locus_tag="OO544_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HCR6767586.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQLQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(5071..5607)
                     /locus_tag="OO544_001981"
     CDS             complement(5071..5607)
                     /locus_tag="OO544_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HCR6767587.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            5754..6422
                     /gene="hxpB"
                     /locus_tag="OO544_001982"
     CDS             5754..6422
                     /gene="hxpB"
                     /locus_tag="OO544_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HCR6767588.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLRDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            6585..7175
                     /locus_tag="OO544_001983"
     CDS             6585..7175
                     /locus_tag="OO544_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR6767589.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            7308..8699
                     /gene="tcyP"
                     /locus_tag="OO544_001984"
     CDS             7308..8699
                     /gene="tcyP"
                     /locus_tag="OO544_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HCR6767590.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLAA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGILLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHR"
     gene            complement(8703..9519)
                     /locus_tag="OO544_001985"
                     /pseudo
     CDS             complement(8703..9519)
                     /locus_tag="OO544_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843061.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9796..10059)
                     /gene="cedA"
                     /locus_tag="OO544_001986"
     CDS             complement(9796..10059)
                     /gene="cedA"
                     /locus_tag="OO544_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HCR6767591.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            10242..12503
                     /gene="katE"
                     /locus_tag="OO544_001987"
     CDS             10242..12503
                     /gene="katE"
                     /locus_tag="OO544_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HCR6767592.1"
                     /translation="MSQHNEKNLHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSADGSFMDELLTLMAAHR
                     VWSRIPKIDKIPA"
     gene            complement(12761..13519)
                     /gene="chbG"
                     /locus_tag="OO544_001988"
     CDS             complement(12761..13519)
                     /gene="chbG"
                     /locus_tag="OO544_001988"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HCR6767593.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMYFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNSG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDRSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELDVLTSASLKGAIAQRGYRLGSYRDV"
     gene            complement(13532..14884)
                     /gene="celF"
                     /locus_tag="OO544_001989"
                     /gene_synonym="chbF"
     CDS             complement(13532..14884)
                     /gene="celF"
                     /locus_tag="OO544_001989"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCR6767594.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEDG
                     KEKLDIIFELCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIHDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(14989..15831)
                     /gene="chbR"
                     /locus_tag="OO544_001990"
     CDS             complement(14989..15831)
                     /gene="chbR"
                     /locus_tag="OO544_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HCR6767595.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDVPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIINEIRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLT
                     EFNQ"
     gene            complement(15839..16189)
                     /gene="chbA"
                     /locus_tag="OO544_001991"
     CDS             complement(15839..16189)
                     /gene="chbA"
                     /locus_tag="OO544_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HCR6767596.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(16240..17598)
                     /gene="chbC"
                     /locus_tag="OO544_001992"
     CDS             complement(16240..17598)
                     /gene="chbC"
                     /locus_tag="OO544_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HCR6767597.1"
                     /translation="MSNVIASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAM
                     FVLINNVFLSFGEGAFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFI
                     GMALAEERKVDALAAGLLSVAAFMTVTPYSVGEAYVVGANWLGGANIISGIIIGLVVA
                     EMFTFIVRRNWVIKLPDSVPASVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQII
                     MDTISTPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQ
                     YGSVEAALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLAL
                     PSGIFQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTM
                     PTGLGAFFNTNGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEEREEDIANAL
                     KF"
     gene            complement(17683..18003)
                     /gene="chbB"
                     /locus_tag="OO544_001993"
     CDS             complement(17683..18003)
                     /gene="chbB"
                     /locus_tag="OO544_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HCR6767598.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(18302..18640)
                     /gene="osmE"
                     /locus_tag="OO544_001994"
     CDS             complement(18302..18640)
                     /gene="osmE"
                     /locus_tag="OO544_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HCR6767599.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            18842..19669
                     /gene="nadE"
                     /locus_tag="OO544_001995"
     CDS             18842..19669
                     /gene="nadE"
                     /locus_tag="OO544_001995"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HCR6767600.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSIDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRLETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLAALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPEQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            19899..20786
                     /gene="cho"
                     /locus_tag="OO544_001996"
     CDS             19899..20786
                     /gene="cho"
                     /locus_tag="OO544_001996"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HCR6767601.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQTIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(20746..21321)
                     /gene="ves"
                     /locus_tag="OO544_001997"
     CDS             complement(20746..21321)
                     /gene="ves"
                     /locus_tag="OO544_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HCR6767602.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFAADQVVKAKLTEGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
BASE COUNT         5738 a         5315 c         4939 g         5563 t
ORIGIN      
        1 cgcccttgat tcattgagaa atggaaaagg caaagtcaac ctgattaaac actactcctc
       61 cgttgaatcc atacagcagc atgtcccctt agtccgggac gcggagttca gagcattact
      121 tcgccatcct cctgcaggga gtcgcgttat cgcgagtaag gattttggct tcgctttaga
      181 tattttcttt tgtcgaatga tggcaaacaa tgtcagtcat atgtccgcga ttttatatat
      241 agacaatcat actttgtcag taaggctacg aataaagcag tcagtgtatg gacaattaaa
      301 ttatgttgtg tctgtttacg acccgaacga taccaacgtt gccgtcagag gcacccacag
      361 ggcagcacgg ggctttctct cgcttgataa gttcatcagt tcagctcccg atgctcagac
      421 ctgggctgat aggtatgttc gcaactgtgc aattgctatt cttcctctat tacctgaggg
      481 agttccagtt gctattttag cgggtatcac gacacgaatg ccatttgccc ctatacatcc
      541 atcggcaatg ttgttaataa tggccacagg ccagactcaa tagcttatta cactattcaa
      601 acagttgccc atactccctg aaaaagaaat cattgaaata ataactgcgc agaatagcgt
      661 tggtacacct gctttatttc tggctatgat gaacggacaa actgacaacg taaaaatatt
      721 tatgcaagaa attcagtcac tggtatacaa tcatatcatt catgaagata atctggttaa
      781 attactgcaa actaaaagtg ctaacgaaac acctggactc tatatctcca tgttgtatgg
      841 attcgatgaa ataatcgata tctttctgaa tgcattaacc actccaatag cacaagaact
      901 tttaaacaaa aaaaatggtg atgagtattt tagccatgaa aatacatgat ggtgagccag
      961 gattatacgc cgcaatggaa aataatcacc ctttgtgtgt cacacggttc ctctctaaaa
     1021 ttaatggcat cgcctttaaa tacaagttga gcaaagctaa catcatggat ttattaaaag
     1081 gagctacagc acagggaacc cctgcattat acatcgccat gagcaagggt aatgaagacg
     1141 tcgtgttatc ttatatatcg acgctgggtg cttttgcaaa aaaacattct tttagtcaac
     1201 atcagttatt tacactattg gccgctaaaa atcatgacaa catgtcagct gttcatatag
     1261 ccattcatca taagcattat aaaactgtag aaacatatta tgctgctata aatgtcatca
     1321 gccaaagcct gagttttagt gctgatgaaa taaagacgta tttataacca gctatttacg
     1381 atatatatcg atacataatt ttattttttt cataaaacgc agaaacagtg accatgtgca
     1441 caacgccacc cgacggctgt aataacaaaa aatcccttga tgcctatccc ttttgttaca
     1501 ctccgttatc acgcacaaga gatatgcagg acactggtat gccgactaaa cgctttgata
     1561 aaaaacactg gaagatggtg gtggtgctac tggcaatctg tggcgctatg ttgttgctac
     1621 gttgggcagc aatgatttgg ggctgagcag tgacaaacga agacagccag accgcataac
     1681 acggtccggc agataatttt acatgctgta gcccagcgat aaggttgtcc gacgatcggt
     1741 atgttccggt gcagactctg gaggttcaga gttccatgtg acgttgtaag ccaccttcag
     1801 tccaaaatgt tcattaatag caacatttaa tgcgctttcg gagttcagcg ttgtatcttc
     1861 cgcgccaaag accgaaacac cctgcgtaaa tttagcgttg tcagtcaact gccatgcata
     1921 agccccggag gcatagccca gcggctgggt ttcactggca ttgtcggtat atttgtcgta
     1981 acgcacacct gggccgaatt caaagcggaa actgtgtacc gggccattca aaaactggcg
     2041 accataaccc gcggtcaaca catcgcgctc tcgatagccg ttataacggt ctgtcagcca
     2101 gcttgcctga ccaaataaat agtcataatc agttaaatta aaacggctac gcccgcccgc
     2161 cgcatatttt tctgaagaac gctcatcatt agaagaagta ttactggcgt tcccccacag
     2221 cgaccaggca gtggtttgtc cataccaggt catggtggtg tcagccgtaa gtgaggagct
     2281 tttcgtattg cctgattgtg caagatatcc tgcgttcaga ttaccttcga aaggtttttt
     2341 agcgctggca ggatcgtcca tgacagtaaa aacggaatca tcggcagctg cattcagtga
     2401 cgcaaacatg ccccccgcca acataacgat ggcaggaact gtcttcaaaa gcttcattta
     2461 tcaagagtcc gtacaacaaa aaaagagacc atcgcggtcc cggaaacttt cttaaggatc
     2521 aaagattagc gtccctggaa aggtaacgaa ttataaaaag gcgcgaataa cttagcaatg
     2581 tattcttatt tcattttttg aataagcatg tggcgaaaac agatttttat ttatatatat
     2641 ttatctgcaa aattttaaat aaagctccaa taaatcatat tgttaatttc ttcactttcc
     2701 gctgattcgg tgccagactg aaatcagcct ataggaggaa atgatggtac gtatctatac
     2761 gttgacactt gcgccctctc tcgatagcgc aacaattacc ccgcaaattt atcccgaagg
     2821 aaaactgcgc tgtaccgcac cggtgttcga acccgggggc ggcggcatca acgtcgcccg
     2881 cgccattgcc catcttggag gcagtgccac agcgatcttc ccggcgggtg gcgcgaccgg
     2941 cgaacacctg gtttcactgt tggcggatga aaatgtcccc gtcgctactg tagaagccaa
     3001 agactggacc cggcagaatt tacatgtaca tgtggaagca agcggtgagc agtatcgttt
     3061 tgttatgcca ggcgcggcat taaatgaaga tgagtttcgc cagcttgaag agcaagttct
     3121 ggaaattgaa tccggggcca tcctggtcat aagcggaagc ctgccgccag gtgtgaagct
     3181 ggaaaaatta acccaactga tttccgcttc gcaaaaacaa gggatccgct gcatcgtcga
     3241 cagttctggc gaagcgttaa gtgcagcact ggcaattggt aacatcgagt tggttaagcc
     3301 taaccaaaaa gaactcagtg cgctggtgaa tcgcgaactc acccagccgg acgatgtccg
     3361 caaagccgcg caggaaatcg ttaatagcgg caaggccaaa cgggttgtcg tttcccttgg
     3421 tccacaagga gcgctgggtg ttgatagtga aaactgcatt caggtggtgc caccaccggt
     3481 gaaaagccag agtaccgttg gcgctggtga cagcatggtc ggcgcgatga cactgaaact
     3541 ggcagaaaat gcctctcttg aagagatggt ccgttttggc gtagctgcgg ggagtgcagc
     3601 cacactcaat cagggaacac gtctgtgctc ccatgacgat acgcaaaaaa tttacgctta
     3661 cctttcccgc taacaaaaac attcccccag cattggggga atcaccacca acctgtcggc
     3721 aacgcgtttc tccgactttg ctcaaaagtc atgtgataac aaaggggtga actatggcca
     3781 gtggcgatct tgtccgttat gtcataaccg tcatgttgca tgaggataca ttgactgaaa
     3841 ttaacgagtt gaataattac ctgactcgcg acggtttttt gctcaccatg acggatgatg
     3901 agggaaatat ccatgagctg gggactaaca cttttggact tatcagtacc caaagtgaag
     3961 aagaaattag agaactggtt tcggggctta cccaaagtgc aaccggcaaa gatcctgaaa
     4021 tcaccatcac gacttgggag gaatggaata gcaacagaaa ataaatggtt tttgggcaat
     4081 aatcagtctg tggtgtgcgt tagctcgtgt ttttacaccg cattcttgcg ctaaccttat
     4141 gatctggcag acaacatggg agagacatca tgtggcaggc aatcagtcgt cttttgagcg
     4201 agcagttagg tgaaggcgaa atcgaactgc gtaatgaact gcctggcgga gaagtccatg
     4261 ccgcatggca tttgcgctat gcaggacatg actttttcgt caaatgtgat gaaagggaac
     4321 tgcttcccgg ttttaccgcc gaagccgacc aactggagtt actgtcgcgt agtaaaaccg
     4381 tcaccgtgcc taaggtttgg gcagtaggcg ctgaccgtga ctacagtttt ctggtgatgg
     4441 attatctccc acctcgtccg ctggatgcgc atagcgcatt tattcttggt cagcaaattg
     4501 cgcgtttaca tcaatggagt gaccaaccac aatttggcct cgattttgat aactcgctct
     4561 ccacaactcc acagcccaac acctggcaac gtcgctggtc aacgtttttt gctgaacaac
     4621 ggattggctg gcagttggaa ctggcagcag agaaagggat cgctttcggc aatatcgacg
     4681 ccatcgtcga gcatattcag cagcgtctgg cctcgcatca actgcagcct tctctgttgc
     4741 acggcgattt atggtccggc aactgtgcgc tgggtccgga tggcccgtac attttcgacc
     4801 ctgcctgcta ctggggtgac cgagagtgcg acctggcgat gttaccgctg catactgaac
     4861 agccgccaca aatctatgac ggctatcagt cagtatcccc gctacctgcc gatttccttg
     4921 aacgtcaacc ggtttaccaa ctctacacgc tgttaaatcg tgcaagatta tttggcggtc
     4981 agcatttggt tattgctcag cagtcattgg atagattatt agcagcatga tatgggttga
     5041 ggataatggc cgctccgtgc ggccttttga ttaaataaac ccaagcagag aaaagaacac
     5101 ataacctgca gcaatacaaa taaccggcag gatatatagt gagaaaaact gcaggaaaat
     5161 agtatggtgc ggaacaacaa tacgggactc aatttgttca cgcgttagcc cctcttcccc
     5221 tttggctttt tccagaatga gttgatcttc aacaccttcc cgcaagaagc gcgcctggcg
     5281 actcatccgg gcaccggaat cctgcaacgc cagtccgaca aaaatcagga tgaaaatcac
     5341 ccagaacata acgttcacgc caccattaaa attaggggtc ggggagttat accagaacag
     5401 attcaaaaaa ggcgtattcg cctgcatcat atcgatcatg acatgagtaa agtcgagcat
     5461 gaccgcatta atgccttcct ggttttcctg ccgggtattc ataaatttca gcaaagaaat
     5521 caatgtggag atcaatgcag gtataaaaat cacccaaccc aaaatccttt tcaaaacagc
     5581 aatgcgtcca gcttgttgat acgtcatgag ttctccttga ttaagacgcg tcgtttcact
     5641 tagtttacct gtagatatct gttttcgccc attctttaaa ggcgatatga taggcgctta
     5701 atcataagca cggcttaata ccttacacat aatgctctaa aggagaggtt gtaatgtcaa
     5761 ccccgcgtca gattcttgct gcaatttttg atatggatgg attacttatc gactcagaac
     5821 ctttacggga tcgagccgaa ctggatgtga tggcaagcct gggggtggat atctcccgtc
     5881 gtaacgagct gccggacacc ttaggtttac gcatcgatat ggtggtcgat ctttggtacg
     5941 cccggcaacc gtggaatggg ccaagccgtc aggaagtagt agaacgggtt attgcccgtg
     6001 ccatttcact ggttgaagag acacgtccat tattaccagg cgtgcgcgaa gctgttgcgt
     6061 tatgcaaaga acaaggttta ttggtgggac tggcctccgc gtcaccacta catatgctgg
     6121 aaaaagtgtt gaccatgttt gacttacgcg acagtttcga tgccctcgcc tcggctgaaa
     6181 aactgcctta cagcaagccg catccgcaag tatatctcga ctgcgcagca aaactgggcg
     6241 ttgacccact gacctgcgtg gcgctggaag actcggtaaa tggcatgatc gcttctaaag
     6301 cagcccgcat gcgttccatc gtcgttcctg cgccagaagc gcaaaatgat ccacgttttg
     6361 tattagcaaa cgtcaaactc tcatcgctga cagaactcac cgcaaaagac cttctcggtt
     6421 aatgaccagg ggcagtgatc acgctgcccc tggttcttta tctgaatttg cattcaactg
     6481 acggattaat cgtcaattta agagaaagag ttacaccgtc accacttccg tgcactgtat
     6541 aaaaatccta tactgtacgt atcgacagtt tagtgagttt tatcatgacg gcggaaggtc
     6601 accttctctt ttctattgct tgtgcggtat ttgccaaaaa tgccgagctg acgcccgtgc
     6661 tggcacaggg tgactggtgg catattgtcc cttccgcaat cctgacgtgt ttgttaccgg
     6721 acatcgatca cccaaagtcg tttcttgggc agcgattaaa atggatatca aaaccgatcg
     6781 cccgcgcttt tgggcatcgt ggttttaccc acagtctgct ggcggtattt gcgctgctgg
     6841 caacctttta ccttaaggtt ccggaaggct ggttcattcc ggctgatgcg ctacaaggaa
     6901 tggtgctggg ttatttgagc cacatacttg ccgatatgct gacacccgcc ggtgttcccc
     6961 tgctctggcc atgccgctgg cgtttccgct tgcctatcct ggttccccaa aagggcaacc
     7021 aactggaacg ttttatctgc atggcattat ttgtctggtc ggtatggatg ccccattcat
     7081 tacccgagaa cagcgctgtt cgttggtcat cgcaaatgat caataccttg cagatccagt
     7141 ttcatcggct tattaagcat caggttgaat actaaaaagg caaaaatcac ctttctggaa
     7201 taagcaattc catttgaata taagagccag ctcacagttc tgttaatctt gcgccaacac
     7261 tatgactgct acgcagtgat agaaataata agatcaggag aacggggatg aactttccat
     7321 taattgcgaa catcgtggtg ttcgttgtac tgctgtttgc gctggctcag acccgccata
     7381 aacagtggag tctggcgaaa aaagtgctgg tgggtctggt gatgggtgtg gtttttggcc
     7441 ttgccctgca taccatttat ggttctgaca gccaggtact taaagattct gtacagtggt
     7501 ttaacatcgt tggtaacggc tatgttcaac tgctgcaaat gatcgttatg ccgttagtct
     7561 tcgcctctat tctgagcgcg gttgcccgtc tgcataacgc atctcagtta ggcaaaatca
     7621 gttttctgac catcggtacg cttttgttta ccacgctgat tgcggcgctg gtcggtgtgc
     7681 tggtcaccaa cctgtttggt ttgacggctg aaggtctggt tcagggtggt gcagaaactg
     7741 cacgtctgaa cgccattgaa agtaactatg ttggtaaagt ctctgatctg agcgttccgc
     7801 agctggtctt gtcctttatc ccgaaaaacc cgtttgccga tcttaccgga gccaatccga
     7861 cgtcaattat cagcgtggta atttttgccg cattcctcgg cgtagctgca ttgaaactgc
     7921 tgaaggatga tgcgccgaaa ggtgaacgcg tcttagccgc tatcgatacc ctacaaagct
     7981 gggtgatgaa actggttcgc cttgtcatgc agttgacccc ttacggcgtt ctggctctaa
     8041 tgaccaaagt ggttgcaggt tctaacttgc aagacatcat caaactggga agtttcgttg
     8101 tcgcgtccta cctcggtctg ctgattatgt ttgcagtgca tggcattctg ctgggcatta
     8161 atggcgtgag tccgctgaag tacttccgta aggtatggcc tgtgctgacg tttgccttta
     8221 ccagccgttc cagtgctgcg tctatcccac tgaatgtgga agcacaaacg cgtcgtctgg
     8281 gcgttcctga atccatcgcc agtttcgccg cctctttcgg tgcaaccatt ggtcagaacg
     8341 gctgcgccgg tttgtatccg gcaatgctgg cggtgatggt tgcgcctacg gttggcatta
     8401 acccgctgga cccgatgtgg attgcgacgc tggtcggtat tgttaccgtt agttccgcag
     8461 gcgttgccgg tgtcggtggt ggtgcaactt tcgccgcact gattgtgctg cctgcgatgg
     8521 gcctgccagt aaccctggtg gcgctgttaa tctccgttga accgcttatc gacatgggcc
     8581 gtacggcgct aaacgttagt ggctcgatga cagctggcac gctgaccagc cagtggctga
     8641 agcaaaccga taaagccatt ctggatagcg aagacgacgc cgaactggca caccgttaat
     8701 tcttatgctg gcaacggtcc gttttgtata ggggccgttg ctttactttt taaatgttcc
     8761 atcgcggcga tgcatactga ctcatattta aacagatcgt cagcgaaaaa aatgtccaac
     8821 ttatcaatac attcctggct caatgaatgt gcctgtagtg agaagcttaa aacctcatac
     8881 ccgagacgtt ttctaagtga actatctgga atactcataa tatcttcaag cacaccactt
     8941 cccattcccg gaagatttag tggattaaaa agaagattga tattaagcat tgatattatc
     9001 gaaggaaact ttaggagact ctcatttttt ttgaatcgca gcaaaaatgc tcccggcagg
     9061 atgtatataa tttttttcat agtatatagt ccttaaataa tgaataacca acctattttt
     9121 tatattgatc aaattcgtca ttttttcggg tcggaattca caggtaaaat ccatcccaac
     9181 aaaattctca ttaatatctc ttatccgaaa ctcactgcca taaagattat taattatcac
     9241 tctatattta acattgtcct gcagatacgt atcaaaacta agtgctggag ctcctggctt
     9301 tgaggcatca tagttaatgt tagacatata ggcaatcata cgattgaagt tcgactctgc
     9361 tatttcatcg cagtcacatt gacccgttga tataaagaaa gtgagtgccg ccatggtaga
     9421 ctcaagcctt tctttaaaag agtcgaaagc caggctttta atatttaaat caccataatt
     9481 actctgtatt aagtttgtag aaaacatctc ccgccccata ttgttaacaa aattattatc
     9541 tcatttaaat ctaagtcact tacaatataa gtttaagagc gacgccacag gatgaactat
     9601 caaaaatagc tcatcatgat tagcaaaact taaccatttt aaaataaata aacaattaaa
     9661 gaaaaaagat cacttattta tagcaatagc tcgtcaaagg cagctttttg ttacaggtgg
     9721 tttgaatgaa tgtagcaacg aaatgcagaa tttcaggtca tgtaactccc ggcaaaaccg
     9781 ggaagtatgt aatccttact cagtcacttc cccttcctgg cggatctgat ttgcccaacg
     9841 ttgggcagat tcaggcacag taaacgccgg tgagcgcaga aatgactctc ccatcagtac
     9901 aaacgcaaca tatttgccac gcagcatcca gacatcacga aacgaatcca tctttatcgc
     9961 atgttctggc ggcgcgggtt ccgtgcgtgg gacatagcta ataatctggc ggttttgctg
    10021 gcggagcggt ttcttcatta ctggcttcac taaacgcata ttaaaaatta gaaaaactgt
    10081 agtttagccg atttagcccc tgtacgtccc gctttgcgtg tatttcataa caccgtttcc
    10141 agaatagtct ccgaagcggg atctggctgg tggtctatag ttagagagtt ttttgaccaa
    10201 aacagcggcc ctttcagtaa taaattaagg agacgagttc aatgtcgcaa cataacgaaa
    10261 agaacctaca tcagcaccag tcaccactac acgattccag cgaagcgaaa ccggggatgg
    10321 actcactggc acctgaggac ggatctcatc gtccagcggc tgaaccaaca ccgccaggtg
    10381 cacaacctac cgcccctggg agcctgaaag cccctgatac acgtaacgaa aaacttaatt
    10441 ctctggaaga cgtacgcaaa ggcagtgaaa attatgcgct gaccactaat cagggcgtgc
    10501 gcatcgccga tgatcaaaac tcactgcgtg ccggtaaccg tggtccaacg ctgctggaag
    10561 attttattct gcgcgagaaa atcacccact ttgaccatga gcgcatcccg gaacgtattg
    10621 ttcatgcacg cggatcagca gctcacggtt atttccagcc atataaaagc ttaagcgata
    10681 tcaccaaagc ggatttcctc tcagatccga acaaaatcac cccagtattt gttaggttct
    10741 ctaccgttca gggtggcgct ggctctgcag ataccgtgcg tgatatccgt ggttttgcca
    10801 ccaagttcta tactgaagag ggtatttttg acctcgttgg caataacacg ccaatcttct
    10861 ttatccagga tgcgcataaa ttccccgatt ttgttcatgc ggtaaaacca gaaccgcact
    10921 gggcaattcc acaagggcaa agtgcccacg atactttctg ggattatgtt tctctgcaac
    10981 ctgaaactct gcacaacgtg atgtgggcga tgtcggatcg cggcatcccc cgcagttacc
    11041 gcaccatgga aggcttcggt attcacacct tccgcctgat taatgccgaa gggaaggcaa
    11101 cgtttgtacg tttccactgg aaaccactgg caggtaaagc ctcactcgtt tgggatgaag
    11161 cacaaaaact caccggacgt gacccggact tccaccgccg cgagttgtgg gaagccattg
    11221 aagcaggcga ttttccggaa tacgaactgg gcttccagtt gattcctgaa gaagatgaat
    11281 tcaagttcga cttcgatctt ctcgatccaa ccaaactgat cccggaagaa ctggtgcccg
    11341 ttcagcgtgt cggcaaaatg gtgctcaatc gcaatccgga taacttcttt gccgaaaacg
    11401 aacaagcagc attccatcca ggtcatattg ttcctggtct ggacttcacc aacgatccgc
    11461 tgttgcaggg acgtttgttc tcctataccg atacacaaat cagtcgtctt ggtgggccga
    11521 atttccatga gattccgatt aaccgtccga cctgccctta ccacaacttc cagcgtgatg
    11581 gcatgcatcg tatggggatc gacactaacc cggcgaatta cgaaccgaac tcgatcaacg
    11641 ataactggcc gcgcgaaaca ccgccgggac cgaaacgcgg cggttttgaa tcatatcagg
    11701 aacgcgtgga aggaaataaa gttcgtgagc gcagtccatc gtttggcgaa tattattccc
    11761 atccgcgtct gttctggcta agtcagacgc cattcgagca gcgccatatt gtcgatggtt
    11821 tcagttttga gttaagcaaa gtcgttcgtc cgtatattcg tgagcgcgtt gttgaccagc
    11881 tggcgcatat tgatctcact ctggcccagg cggtggcgaa aaatctcggt atcgaactga
    11941 ctgacgacca gctgaatatc accccacctc cggacgtcaa cggtctgaaa aaggatccat
    12001 ccttaagttt gtacgccatt cctgacggtg atgtgaaagg tcgcgtggta gcgattttac
    12061 ttaatgatga agtgagatcg gcagaccttc tggccattct caaggccctg aaggccaaag
    12121 gcgttcatgc caaactgctc tactcccgaa tgggtgaagt gactgcggat gacggtacgg
    12181 tgttgcctat agccgctacc tttgccggtg caccttcgct gacggtcgat gcggtcattg
    12241 tcccttgcgg caatatcgcg gatatcgctg acaacggcga tgccaactac tacctgatgg
    12301 aagcctacaa acaccttaaa ccgattgcgc tggcgggaga cgcgcgcaag tttaaagcaa
    12361 caatcaaggt cgctgaccag ggtgaagaag ggattgtgga agctgacagc gctgacggta
    12421 gttttatgga tgaactgcta acgctgatgg cagcacaccg cgtgtggtca cgcattccta
    12481 agattgacaa aattcctgcc tgatgggagg cggcgcaatt gcgccgcctc aaatgattta
    12541 catagtgcgc tttgtttatg ccggatgcgg cgtgaacgcc ttatccggcc tacaaaactg
    12601 tgcaaattca atatattgca ggaaacacgt aggcctgata agcgaagcgc atcaggcagt
    12661 tttgcgtttg tcagcagtct caagcggcgc agttacgccg cctttgtagg aattaatcgc
    12721 cggatgcaag gttcacgccg catctggcaa acatcctcac ttacacatcc cgataactcc
    12781 ccaaccgata accacgctga gcgatagcac ctttcaacga cgctgatgtc aacacatcca
    12841 gctccgttaa gcgtgggaaa cagtaagcac tctgacggat agtattatcg ataaacgccg
    12901 gatgacacat cacctccagc gaacgatcac cccgatggct tgcgtcatcc agcacttgca
    12961 ggaacagtgc ctcgctaatc tcttcaccat aaaacgcgct actgaatccc tgagaactgc
    13021 gcagattagc cggtaaatca ccagagttag atagcggctg acggtcgatg cgcaacgcaa
    13081 tcccctcttc agccgcaaac ctggcaacaa tcgggaaaat ctgcgggaac atatgcacat
    13141 gatgatggct atcaagatgc gtaggtttgc gtccaaatag ctcaatgaaa cgcaaatact
    13201 gactggcaag ctcctgagta atttcttcca gcggtaaagc atcttcttct gccaactgcc
    13261 agatccattt tcccagcaca ccatcgcggg ttaaccccgg catagctgtc agtggcttgc
    13321 ccatagtaag gacaaagtac atccctatgg ccagactcgg ttcatcacga ctcaactgca
    13381 ccgcatggtc aatagcctgc ccattcacca gcgccgtcgt cgatgtgaca atcccattgc
    13441 gacaggcctc gataatgccg tagttctgac ctttgcttaa gccaaaatca tcggcattaa
    13501 caatcagtaa gcgttccata atcagcctcg gttaatgtgc ttttttaagc tctgcgatgc
    13561 agtcggcaaa atttggcagc catttctcgt gcgccagaat catctcgcgt gccagcagct
    13621 cagcatcgcg atcggaatgc accaacggac taaggtttag cgccagtaac acatcgttaa
    13681 attctccgct aagtgcggcg ttgctggcag caatctcgaa gcctttaatg gtgtgaatca
    13741 gccccatcac tttatcatcg aaatgcgtaa tgcgcggatg tggcgtcgcg ccatcgcgcc
    13801 ccagcttaca ggtcatttct accgcccagt ctgccggaat attatcaata tgcccatgat
    13861 gcgggatatt aacgtaatgt tcagcttgct tgtcgttgta gatagcgttg atcacttcgc
    13921 acgctgcatc agagtaataa gccccaccgc gctgttccag ttctttcggc ttaactttca
    13981 actccgggtt tttatacagc tcaaaaagtt gtttctcgac tttctgtact acctgcgctc
    14041 gtgcgccgcc tttgtagtat tcgcccattt caatagccag catctctttt tgcttgaagt
    14101 aataaagcaa ataagaacac ggcaacagat tcagagaacg aattaagcct tcgctaaatg
    14161 gcagatcgaa aatattttta acgccagatg cttttaactg cccggatgcc acaccatcaa
    14221 gcaattcggc aaagcgcgac ttgccattta ccagcacatc cttaatgaac accatatggt
    14281 tgaggccgaa caaatcgata gataaatcat cgctgtcttt cagcatcaga acatcgtgaa
    14341 taaacatctt catgccgatc ggaatattac acacgccgat aaagcgttta aatccggtat
    14401 gacgataaac ggcttcagta accattcccg ccgggttagt gaagttaatc acccatgcat
    14461 tcggacaaag ttcttcgaca tcttttacga tgtcaaaaat caccggaatg gtacgcagac
    14521 ctttaaacag accgcccgcg ccgttggttt cctggccaag ataaccatga cttaatggaa
    14581 tacgttcatc cagttcgcgc gccggtaatt ggcctacgcg cagttgggta gtaacgaaat
    14641 cagcatcttt caatgcttcg cggcgatcca gcgttttata aagcttcatc gggacgccag
    14701 cgttatcaat catccgttgg cagagttcaa aaatgatatc cagtttctct ttaccatctt
    14761 cgacatccac cagccataat tcgctgaccg gcaattcgtg ataacgctta ataaatcctt
    14821 ccagtaactc cggggtatag ctgctcccgc caccaatagt gacgactttt aatttctggc
    14881 tcataatttc tcccttcagt acagaatact gatatctggc atatctgccc ccccggacat
    14941 aaataatcca gcaacaggac agatatgtga attgtcaggt ataacgactt actgattaaa
    15001 ttcagtcaat ttcttacgat agctcttagg cgtaaaggac gttaatttct taaacgtttt
    15061 aataaacaaa ctcgggctac tataaccggc ctcaaacgca atatccgtca cggaatagtt
    15121 ggtcatttcc agttgttttt tggcaaaatt aatacggatc tcattaataa tctgcattgg
    15181 tgttttgcca taatatcgtt gagtcgctcg cgtcaaatat tcctgtgatt tggctgacaa
    15241 cgtcaccata ttctccagcg ccgattcact aaactgctct ttatcatgca tcttttctac
    15301 cgtacttttc agccactgcg gtacatcatc aatcacctgt tcttcgcggt aatgacgtaa
    15361 acggttaatg acataaaaag taaccatctc aacaaactct tccagccctg tttcgcggaa
    15421 attcaatgaa gaaatcactg tttccacata ggtgagaaaa gcgttattgg tccggtatac
    15481 ctgcgaagcg acaaagcaat aaggcaacaa cggcaggtaa tgctgctcaa aaaagcgttt
    15541 actgatccca acgttcaata tacgcgtggc accaaactca taaaaacttt gatggtgcga
    15601 acctaacgga ataaaaacaa aatcgccccg ttccagtaac acgcgcttac cgttaatctc
    15661 ctggaaataa cgcccggtta ataccagagt aaattcataa tagtcgtgct gatgcagccc
    15721 gctgatactc tcagttttgt tatagataaa cacatggaag tttttgccat taaacaactg
    15781 ctgttctcgg gcagtggcaa tttccggcgc gttaatcact ggctgcatca tcgactcctt
    15841 atgccttcag tttttcatga agctcaatta attcagtaat cagttcacgc gcaagcatgg
    15901 acgtcattaa atgatcctga gcgtggacca gcaccagact cactttcatc ttaccttcgc
    15961 ccgcatcgcc ttcaatcagt ttcgtctgta ccagatgcgc ttcattcaat gccatccgtg
    16021 actgatccat catggctttt gctgcagcaa aatcgccctg cttcgcctgt ttcagtgccg
    16081 catacgccag gctgcgcgct tgtccggagt tgatgataag ccccatcacc acttcttcca
    16141 gctcttcagc ttccgtttgc gtatcgggaa tgttatcgag atccatcata catcgttcct
    16201 ctctattctt accggcacga ttacccgtac cggcatcgat taaaatttca gggcgttagc
    16261 gatatcttct tcgcgctctt ctttatcaat cgcgttttgt gctttgttag ccaccacaac
    16321 aaagggcaga taaattaacg ttgcgatacc aaggttgaag agtgcaacca gcaatgcggc
    16381 gacgctaccg ttggtgttaa agaaggctcc cagaccggtt ggcatggtcc acggtgcaat
    16441 attggtcacc ggaggaatga tgcccatgta gtacgctgcg agggtgattg ccgccagaat
    16501 cggttgtacc agtacaaacg ggataaacat caccgggttc atgataattg gcagaccaaa
    16561 cagaatcggt tcgttaatct ggaagatgcc ggacggcagc gccagttttg ccacctgacg
    16621 ataatcagca cggcgagagg cgataaagat agccaggatc aggcctaaag tcgcaccact
    16681 gcccccaagg aaaataaagg agtccagcat cggcttggcc cagatatgga aggtcttacc
    16741 ggctgccagc gccgcttcga cggaaccata ttgctgatag gtcgcgatat tttccagcgc
    16801 ccacggcgtc ataatgccgt tgtccagtgc ggtcagcgcc agcgcgccat gaataccgaa
    16861 gaaccagagc agtggaacaa agatcacata ggcccagccc accacgctac ccaacgatgc
    16921 cagtggggtt gagatggtat ccataatgat ctgatggaag ttggtgcccc aggtattcaa
    16981 cgcccaggca ataatcccca tcacggaaag aataataaag ccgggaatta atgccgagaa
    17041 ggaacgcgat actgaagcag gtacgctatc gggcaattta atgacccaat tgcggcggac
    17101 aataaaggta aacatttctg ccaccaccag gccaataata atcccggaga tgatattcgc
    17161 tccacctaac cagtttgcgc caaccacata ggcctcaccg acactatatg gggtgacggt
    17221 cataaatgct gcaacggata acaacccagc cgccagcgca tcgactttac gctcttctgc
    17281 cagcgccatg ccaataaaga acggtgccat taaagacatt atccctaatg ttccgttata
    17341 tacgttgccg ccaatacctt tcaggccatt aagtgtttca atggttgagg cgtcgaggcg
    17401 aatacctaag gaataaaaaa acgccccctc cccaaagctt agaaaaacgt tgttaattaa
    17461 tacaaacatg gcccccgcaa gggttaacgg cattaagcga ataaagccat ttttgattgc
    17521 attaacgtgt ggctgctttc ctattttaac tgcaaaaggg aggagtacct tttcaagcga
    17581 tgcaataaca ttactcatag aaaaataccc ttaaaaaccg caatttaaat attgcggtat
    17641 tgatttatga aataactctt tgacgggaaa atttaaaata aattaatttg ctgcggcttt
    17701 tttaatcgct gcaaccgcag ccttaagcac gcctaaacca tcgactttgc cataaagcag
    17761 cgagtcaatt acttcaaccg gtttgttggg taacaaacgc tggatttcgg gcaacatata
    17821 agcaatctgc ggccctaata acacgacatc ggcattctga cctttttcac cagccagtgt
    17881 ttccggaaat gcttcaataa tgaccggaac ttcatatttt tctgcctgtg cgcgcatttt
    17941 tgataccagt aaagaggtag acatgcccgc agaacaaaac agataaatgt gtttcttttc
    18001 cataaaactg ccctcatcga cgattatctg tcagccagac actccgcaag ccttaacctg
    18061 cttccatgct ctgggtaact tgcgaaacca acatgacttt ttttgtggct gaaaggagta
    18121 tacggtatag acctgagcaa tagtatttct ttgaccttat aaattgtttc tccttgacct
    18181 gtggttatga ccctcttcac atttcgggca aataattcgc gaagataatt aagaactcag
    18241 gccaaaaaaa accggcgcaa tggccggttt ccgttgttac tcaagctttc agacgaattg
    18301 attacttcgc agcctgtgga tcagtgtcgt attcagcaca ggtctgataa ccggagttga
    18361 tgacatgtcc ggtatcatct aacgcgacaa agtaggtttc tgctttacca tcacgttgac
    18421 ccaggatgta ggtctggcaa gtaccacgag catggatcat gctcacttca gacgaaggtt
    18481 tacccgcaat ttgtgcaacc tgcgcccggc tcatgccttt tttgacgtct ttcaccacag
    18541 gctgtacaaa ctggtcttta gtacgatcat aagccgtaca acccgccagc atggttaata
    18601 ccgccgctgc actcagaatt cctgccatat tcttgttcat attccgtcct cttgtttatc
    18661 agcgtgttag ataagcctgg aatacattgg gcgctttttc aagcccgcga acgaaacggc
    18721 tccgctttca gaggattcct gtatgacgtt ttaaccacca ttcagcccgc tgtcgcttgt
    18781 cgtttcagtg gcaacgggtt agctttaagg aagttttgtc ttttctgtct ggaggggttc
    18841 aatgacattg caacaacaaa taataaaggc gctgggcgca aaaccgcaga ttaatgctga
    18901 agaggaaatt cgtcgtagta tcgattttct gaaaagctac ctgcaaactt atccgttcat
    18961 taaatcactg gtgctcggga tcagcggcgg tcaggactcc acgcttgccg gaaagctgtg
    19021 ccagatggcg attaatgagc tgcgcctgga aaccggcaac gaatcactgc aatttattgc
    19081 cgtacgcctg ccctatggtg ttcaggccga cgaacaagat tgccaggatg ccattgcctt
    19141 tattcaaccg gatcgcgtat taaccgttaa tatcaagggc gcggtattgg ccagcgaaca
    19201 ggcattgcgg gaagcaggca ttgaactgag cgattttgtc cgtggcaatg aaaaagcgcg
    19261 tgagcggatg aaagcacaat atagcattgc gggtatgacc agcggtgtcg tggtgggcac
    19321 cgatcatgca gcagaagcca ttaccggatt cttcactaaa tatggtgacg gcggtacaga
    19381 cattaatccg ctgtatcgtc tcaacaaacg tcagggtaaa cagttactgg cggcattagg
    19441 ttgcccggaa cacctttata agaaagcgcc aacggccgat ctggaagatg atcgcccttc
    19501 tctgccagat gaagtggcac tcggcgtgac ctatgacaat atcgacgact atctggaagg
    19561 gaaaaacgta cctgaacagg tcgccagaac aatagagaac tggtatctga aaaccgaaca
    19621 taaacgccgt ccgccaatta ccgttttcga tgatttctgg aaaaagtaat aatttgcaca
    19681 taatggtttg tgcgaaaaag agtaatttgt tcacgccgga tgcggcgtga acgccttatt
    19741 cgacctacaa aactatgcaa attcaatata ttgcaggagt cgaataggcc tgacaggcgt
    19801 agcacgtcag acggtgtaac ctttgtcatc gacccgcctc ttttttaatc gctccccgcc
    19861 tgttacactg gatagataac cagcattcgg agtcaacagt ggtacggcgt ttaacttctc
    19921 cgcggctcga atttgaagct gcggcaattt atgaatatcc cgaacattta cgttcattcc
    19981 ttaatgactt acccacccga ccaggggtgt atctgtttca tggcgaaagt gacaccatgc
    20041 cgctctatat cggcaaaagc gttaatatcc gcagtcgggt gctttctcat ttacgcaccc
    20101 cggatgaagc cgccatgcta cggcaatccc ggcgaattag ctggatctgt accgcaggcg
    20161 aaatcggcgc tctgctcctt gaagcgcgat taatcaaaga acaacagccg ctgtttaata
    20221 aacggttgcg ccgcaatcgc cagctctgtg ccctgcaatt aaatgaaaag cgcgtcgatg
    20281 tggtgtatgc caaagaggtg gatttttcac gagcccccaa cctgtttggc ctgtttgcca
    20341 ataggcgcgc agctttgcaa gcattgcaga ccatcgctga tgaacaaaaa ctttgttatg
    20401 gcctgctggg actggaaccg ttaagtcgcg gtcgtgcatg ttttcgttca gcgctaaaac
    20461 gttgcgccgg agcatgctgc ggtaaagaga gccatgagga acatgcgcta cgcttgcgcc
    20521 agtctctgga gcgtttgcgg gtggtgtgtt ggccttggca aggtgcggtg gcactgaaag
    20581 aacagcaccc ggaaatgact caatatcata ttattcaaaa ctggctgtgg ctgggggcgg
    20641 ttaattcgct ggaagaagcg acaacgttaa ttcggacacc cgccgggttt gatcacgacg
    20701 gttataaaat tctttgtaag ccgctgcttt ccggtaacta tgaaattact gaacttgatc
    20761 cggcgaatga ccagcgagcc agttgatttc actgaacaac agtttacctt gcggttgtaa
    20821 aagccgcagg gtgtgcctgc catcgaacca gcacgcgccc tggtcggtag tgagcaattt
    20881 atcgcccagt tgccacgccc cgttaatcac aaacaccacc ccgccgcgtg agccaaaggt
    20941 agtaaacgta cgttcggcaa ttctgacttt cgccttacag acatccaggc gtgtcatgat
    21001 gttgaaatcc atcgacatct gcccttccgt cagtttcgct tttaccacct ggtccgctgc
    21061 aaaggcaaaa ggctgtaacg gttttaaggt atggttaaag cggtctgcgc tttcaaggag
    21121 catctcgccg ccttccagca acgtcactat cctttccatg ccgggaaaca aagaaaactc
    21181 cccattcgcc gcgatggatg caatgctggc acgccagtaa aaatcacgtt ttgccggtgg
    21241 gaaagtgcaa atttcgcgcg tttcaccggc agcatttcgc cacagattca ccgacatttt
    21301 acgcatatca aagtattcca tactcgctcc cggcccgttc ttttcattgt tatcgttcag
    21361 gtaaccgtgg atggttatcg gcaactcggg tgatttacgt tagtggtgat caggcttttt
    21421 ttacaactgt ttgagaagag aaaagaaaac cgccgatcct gtccaccgca ttactgcaag
    21481 gtagtggaca agaccggcgg tcttaagttt tttggctgaa agattattca gcagttgcag
    21541 gcattttacc ttttg
//
LOCUS       DAJTZL010000062        21455 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.062, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000062 DAJTZL010000000
VERSION     DAJTZL010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21455
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.062"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(331..876)
                     /gene="hemG"
                     /locus_tag="OO544_001998"
     CDS             complement(331..876)
                     /gene="hemG"
                     /locus_tag="OO544_001998"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HCR6767603.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAQLTNKPTLK"
     gene            complement(888..2339)
                     /gene="trkH"
                     /locus_tag="OO544_001999"
     CDS             complement(888..2339)
                     /gene="trkH"
                     /locus_tag="OO544_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HCR6767604.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            complement(2378..2992)
                     /locus_tag="OO544_002000"
     CDS             complement(2378..2992)
                     /locus_tag="OO544_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HCR6767605.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITSVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYHQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            complement(2992..4323)
                     /gene="pepQ"
                     /locus_tag="OO544_002001"
     CDS             complement(2992..4323)
                     /gene="pepQ"
                     /locus_tag="OO544_002001"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HCR6767606.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVELLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHASVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            4513..6702
                     /gene="fadB"
                     /locus_tag="OO544_002002"
     CDS             4513..6702
                     /gene="fadB"
                     /locus_tag="OO544_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HCR6767607.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLVEGAKAVLRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            6712..7875
                     /gene="fadA"
                     /locus_tag="OO544_002003"
     CDS             6712..7875
                     /gene="fadA"
                     /locus_tag="OO544_002003"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HCR6767608.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQAGLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSGAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVSGTVTAGTSSALSDGAAAMLVMSESRARELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMEHKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(8065..8766)
                     /gene="fre"
                     /locus_tag="OO544_002004"
     CDS             complement(8065..8766)
                     /gene="fre"
                     /locus_tag="OO544_002004"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HCR6767609.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(8813..10306)
                     /gene="ubiD"
                     /locus_tag="OO544_002005"
     CDS             complement(8813..10306)
                     /gene="ubiD"
                     /locus_tag="OO544_002005"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HCR6767610.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            10473..10961
                     /gene="rfaH"
                     /locus_tag="OO544_002006"
     CDS             10473..10961
                     /gene="rfaH"
                     /locus_tag="OO544_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HCR6767611.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASLAIVPSAVIHQLSVYKPK
                     DIVDPATPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            complement(10958..11740)
                     /gene="tatD"
                     /locus_tag="OO544_002007"
     CDS             complement(10958..11740)
                     /gene="tatD"
                     /locus_tag="OO544_002007"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HCR6767612.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAADLNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVAHGIYIGITGWVYDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTRFGIAF"
     gene            complement(11782..12558)
                     /gene="tatC"
                     /locus_tag="OO544_002008"
     CDS             complement(11782..12558)
                     /gene="tatC"
                     /locus_tag="OO544_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HCR6767613.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            complement(12561..13076)
                     /gene="tatB"
                     /locus_tag="OO544_002009"
     CDS             complement(12561..13076)
                     /gene="tatB"
                     /locus_tag="OO544_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HCR6767614.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            complement(13080..13349)
                     /gene="tatA"
                     /locus_tag="OO544_002010"
     CDS             complement(13080..13349)
                     /gene="tatA"
                     /locus_tag="OO544_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HCR6767615.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            complement(13428..15068)
                     /gene="ubiB"
                     /locus_tag="OO544_002011"
     CDS             complement(13428..15068)
                     /gene="ubiB"
                     /locus_tag="OO544_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HCR6767616.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVATLEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            complement(15065..15670)
                     /gene="ubiJ"
                     /locus_tag="OO544_002012"
     CDS             complement(15065..15670)
                     /gene="ubiJ"
                     /locus_tag="OO544_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HCR6767617.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            complement(15684..16439)
                     /gene="ubiE"
                     /locus_tag="OO544_002013"
     CDS             complement(15684..16439)
                     /gene="ubiE"
                     /locus_tag="OO544_002013"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HCR6767618.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            complement(16534..17961)
                     /gene="rmuC"
                     /locus_tag="OO544_002014"
     CDS             complement(16534..17961)
                     /gene="rmuC"
                     /locus_tag="OO544_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HCR6767619.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQNEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            complement(18102..18863)
                     /gene="udp"
                     /locus_tag="OO544_002015"
     CDS             complement(18102..18863)
                     /gene="udp"
                     /locus_tag="OO544_002015"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HCR6767620.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGIMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESQAVKIVVEAARRLL"
     gene            19125..19937
                     /locus_tag="OO544_002016"
     CDS             19125..19937
                     /locus_tag="OO544_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HCR6767621.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTLDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHAASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPILGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGEKRS"
     gene            20331..21392
                     /gene="hcp"
                     /locus_tag="OO544_002017"
                     /pseudo
     CDS             20331..21392
                     /gene="hcp"
                     /locus_tag="OO544_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278761.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
BASE COUNT         5264 a         5740 c         5377 g         5074 t
ORIGIN      
        1 cactcttgag acttggtatt catttttcgt cttgcgacgt taagaatccg tatcttcgag
       61 tgcccacaca gattgtctga taaattgtta aagagcagtg ccgcttcgct ttttctcagc
      121 ggcgcggggt gtgcataata cgctttcccg ctacagagtc aagcatttat ttttgctttc
      181 tctgtcggaa ttctcaggag aaccccgctg acccggcggc gtgtttgccg ttgttccgtg
      241 tcagtggtgg cgcattatag ggagttattc cggcctgaca agaggaaatt taaaataatt
      301 ttctgaccgc gcatttttta ttctttatgc ttatttcagc gtcggtttgt tggttaactg
      361 ggcgatttct cgggcgaaat tcgccacctg ctcccaatcg gtatagacaa cttctttgcg
      421 cgtatccgtt tcaccgcctg acatcttcat aattagcttg atcataaaac gatcgtacca
      481 gcgataacgt gggtaacgca gcgccccggc aatgaccgcg cagcgatcgg gacgccattg
      541 cgagttcatc agaaacttcc gcgcgtagct gttggtctgt ggagtacgct tctccggttt
      601 gcgcgccacc aggtttacgg aataaaaggc gctcggcatc gaattcagtc gcgtcgcatg
      661 ttttttgaca aattcctgga acgcagagtg gtagtgacca tagcgaatag aagcaccaat
      721 gaccacacgg tcataatttt cccattgtgg ttcttcaatg cggtgcacat tggcgacatc
      781 cgtctggatc cccagttctt tcagttccga agccaggtag gaggcaatct cgcgcgtttg
      841 tccgtccctt gttgagaaaa gaattaatgt tttcacgtat tactccatca ttcacgccag
      901 aaagtcgggg taaagagcac cagcaatgta aagacctcga gacgaccaaa cagcatgttg
      961 gcaatcagga tccatttagc caccgggttc atactggtaa agttatcagc aaccacgcca
     1021 agccctggcc ccaggttatt caatgtcgca acaactgaag caaaggcaga aaagtcatcc
     1081 acgcccgtgg cgataatcgc cagcatactg acaataaaca ccaatgcata ggcggagaaa
     1141 aatccccaaa cggcttcgag gatacgttcc ggcagtgcgc gattccccag cttaatgcta
     1201 tagacggcgt tcggatgcac cagtcgtttc agctcacggt tcccctgctt aaacagcagc
     1261 aggatgcgga tcactttcag gccaccgccc gttgacccgg cgcaaccgcc gataaaagct
     1321 gaacataaaa gcagtaccgg caaaaagagc ggccagcggg caatgctgtc agtcgtaaac
     1381 ccggcggttg tcgccatcga taccacctgg aaaaacgcct ggttaattgt catcagcgcc
     1441 gaactgtaga cattatgaaa ccacagtacg agtgtacaaa taaccaccag cgtaaactgt
     1501 acgccgataa acatgcgaaa ttccggatcg cgccaataaa ccttcagact acgcccactt
     1561 aacagtgaaa agtgcagacc gtagttacag ccggagatca gcaggaagat agcaatgata
     1621 gtgttaatag tcgggctgtc gaaataaccg atactggcat catgtgtcga gaagccgcca
     1681 atagcgatag tcgcaaagct atggccgatg gcatcaaagg catccattcc ggcaaaccac
     1741 aacgccagcg cacaggcgac ggtcagcaag acataaatca accacagggt tttcgccgtt
     1801 tccgcaattc gcgggcgcat tttgttatct ttcagcgggc cgggcatttc tgcgcgatag
     1861 agctgcatcc cacccacgcc gaggataggc agtatcgcaa ccgctaacac gatgatcccc
     1921 atcccgccaa accattgcag catctggcga taaaagagga tggcatgagg gagcgaatcc
     1981 agccccacca gcgtagtggc cccggtggtg gtcaggccag agaaagattc aaaaaacgca
     2041 tcggtaatcg tgaggttcgg gctttccgag aagataaaag ggagcgcacc gacactaccc
     2101 agcacggtcc agaacagcac cactatcaga aacccctcac gggatttcag ttcgcccttc
     2161 tctttacggt tcggccacca cagcatagaa ccaatggcga gggcgacaaa aaaggtctgg
     2221 gtaaaagcgc ggcccgctcc atcccggtag atgagtgcta ccagcccagg gataatcatg
     2281 gtccctgaaa ataagatgac cagtagtcca acgattcggg taatggcgcg aaaatgcatc
     2341 tctgccgctt ccttagttct gcaaaacgaa gtggggatta ttcttcaatc gctaacaatt
     2401 gcaatgaacc acggctaaaa tccgccagct ttgcggaaaa ttcagccact ttcgccgccg
     2461 gaagcgccac ccgcagcaga acgaatgcct gataatcact gttgataatt ttgccgtcac
     2521 actgccccag caacgcttca atgccggtta actgatgata ttcacattgc aaagtatatt
     2581 cggttaatgg cgtcttgcgt tgggtcgtta gctggcgcag cgcctgattc acgccgccgc
     2641 catacgcttt cactaaccca ccggtgccaa gcaatatgcc gccgtagtag cgcactacca
     2701 cagaggtaat ttccccgacg ccgctgccca ttagctgggc gagcatcggt ttacctgccg
     2761 ttcctgccgg ctccccgtcg tcagagaaac ccagctgttg agaatcatcc ggcgcacccg
     2821 ccacccacgc cacgcaatgg tggcgggcat cggggtgttc tgcccgcacc gattcaacaa
     2881 acgctttcgc cgcctcaacg ccatcggtat gcgccaacat cgttatgaag cggctttttt
     2941 tgatctcttc aacgaccgtg accggtgccg caggaattaa ccagctttcc atcacgccag
     3001 tttcagatcc cgggtcatgt tttccacgtt gttttcgtgg atcaccacgt tgtcttcgat
     3061 acgaatgccg ccgaacggtt tcagtgcttc aattttctgc cagttgaagt gcttgctgaa
     3121 ctgcccttca cgccacggtg ccagtagcga ttcaatgaag tagatacccg gttcgatggt
     3181 taacaccata cccggctgga gaatacgggt gcagcgcagg tacggatatt ttgccggtgc
     3241 cgcgaggtgt gtaccgctat catcctgcat aaaaccggct acgtcatgca cctgcaggcc
     3301 cagcggatga ccgataccat gcggcataaa cggtccggtg agatcgtttt cgaccatcgc
     3361 ttcttcactc atatcggtga tgatttgatg tttacgcagc agtttggcga tgcgctgatg
     3421 gaactggatg tggtaatcca catagctaac gcctgctttc atggtggcga tcagcgccag
     3481 ttgttcatca tttacgtctt tcaccagctg tgcgtagtcg ttgtcgcttt ttgccgacca
     3541 ggtacgagtc aggtcagccg catagccgtt atattcggcc ccggcatcca gcaggaagct
     3601 gcgcatctct tccggtgcct gatgatccag tttggtgtaa tgcagcaccg aagcgtgttc
     3661 gttaagcgcc acaatgttgc tgtaaggtac gtcggtatca cgatgaccgg tcgcggtcag
     3721 ataggcgatg ttgatatcga actcgctcat gccagaacgg aacgcttctt ctgccgcacg
     3781 atgaccgttg accgccattt tctgcgcttc acgcatacag gccagttcgt actcggtttt
     3841 gaaggagcgg tagtaatgca ggtagtcgat cacccctttc gggttgatgt tgctggcctc
     3901 aatacccagt tgcagcgcac gttccggcac cggaccgata taaccgatat tgccgcgcgc
     3961 agcgggcagc aggctaccaa tgccatcggc tttcggcagt gcgatcactt ccacatcttc
     4021 agtccagaag gaggtcggca gcagttcgac gttgtgccag taatcaaccg gcagatagaa
     4081 ccacagtttc ggcttgttca cgccatccac cagcaaccag cagtttggca cctgagttac
     4141 cggcacccac gctttgaatt gcgggttcac tttaaacgga tagggatgat cgtcgagaaa
     4201 aacgttgaac agctcgccgg agtgaataag taatgcatcc agcttgaagc gcgccagcgc
     4261 atcgcgagtc cgttcttgta aggtagctat atgattttta tagagcgagg ccagtgattc
     4321 cattttttac ccttctgttt ttttgacctt aagtctccgc atcttagcac atcgttcatc
     4381 cagagcgtga tttctgccga gcgtgatcag atcggcattt ctttaatctt ttatttgcat
     4441 atttttaaca caaaatacac acttcgatgc atctggtacg accagatcac cttgcggatt
     4501 caggagactg acatgcttta caaaggcgac accctgtacc ttgactggct ggaagatggc
     4561 attgccgaac tggtatttga tgccccaggt tcagttaata aactcgacac tgcgaccgtc
     4621 gccagcctcg gcgaggccat cggcgtgctg gaacagcaat cagatctaaa agggctgctg
     4681 ctgcgttcga acaaagcagc ctttatcgtc ggtgctgata tcaccgaatt tttgtccctg
     4741 ttcctcgttc ctgaagaaca gttaagtcag tggctgcact ttgccaatag cgtgtttaat
     4801 cgcctggaag atctgccggt gccgaccatt gctgccgtca acggctatgc gctgggtggt
     4861 ggctgcgaat gcgtgctggc gaccgattat cgtctggcga cgccggatct gcgcatcggt
     4921 ctgccggaaa ccaaactggg catcatgccg ggctttggcg gttctgtacg tatgccacgt
     4981 atgctgggcg ctgacagtgc gctggaaatc attgccgccg gtaaagatgt cggcgcggat
     5041 caggcgctga aaatcggtct ggtggatggg gtagtcaaag cagaaaaact ggttgaaggc
     5101 gcaaaggcgg ttttacgcca ggccattaac ggcgacctcg actggaaagc gaaacgccag
     5161 ccgaagctgg aaccgctaaa actgagcaag attgaagcca ccatgagctt caccatcgct
     5221 aaaggaatgg tcgcgcaaac ggcggggaaa cattatccgg cccccatcac cgcagtaaaa
     5281 accattgaag ctgcggcccg ttttggtcgt gaagaagcct taaatctgga aaacaaaagt
     5341 tttgtcccgc tggcgcatac caacgaagct cgcgcactgg tcggcatttt ccttaacgat
     5401 caatatgtaa aaggcaaagc gaagaaactc accaaagacg ttgaaacccc gaaacaggct
     5461 gctgtgctgg gcgcaggcat catgggtggc ggtatcgctt accagtccgc gtggaaaggc
     5521 gtgccggttg tcatgaaaga tatcaacgac aagtcgttaa ccctcggcat gaccgaagcc
     5581 gcgaaactgc tgaacaagca gcttgagcgc ggcaagatcg atggtctgaa actggctggc
     5641 gtgatctcca cgatccaccc aacgctcgac tacgccggat ttgaccgcgt ggatattgtg
     5701 gtagaagcgg ttgttgaaaa cccgaaagtg aaaaaagccg tactggcaga aaccgaacaa
     5761 aaagtacgcc aggataccgt gctggcgtct aacacttcaa ccattcctat cagcgaactg
     5821 gccaacgcgc tggaacgccc ggaaaacttc tgcgggatgc acttctttaa cccggtccac
     5881 cgaatgccgt tggtagaaat tattcgcggc gagaaaagct ccgacgaaac catcgcgaaa
     5941 gttgtcgcct gggcgagcaa gatgggcaag acgccgattg tggttaacga ctgccccggc
     6001 ttctttgtta accgcgtgct gttcccgtat ttcgccggtt tcagccagct gctgcgcgac
     6061 ggcgcggatt tccgcaagat cgacaaagtg atggaaaaac agtttggctg gccgatgggc
     6121 ccggcatatc tgctggacgt tgtgggcatt gataccgcgc atcacgctca ggctgtcatg
     6181 gcagcaggct tcccgcagcg gatgcaaaaa gattaccgcg atgccatcga cgcgctgttt
     6241 gatgccaacc gctttggtca gaagaacggc ctcggtttct ggcgttataa agaagacagc
     6301 aaaggtaagc cgaagaaaga agaagacgcc gccgttgaag acctgctggc agaagtgagc
     6361 cagccgaagc gcgatttcag cgaagaagag attatcgccc gcatgatgat cccgatggtc
     6421 aacgaagtgg tgcgctgtct ggaagagggc attatcgcca ctccggcgga agcggatatg
     6481 gcgctggtct acggcctggg cttccctccg ttccacggcg gcgcgttccg ctggctggac
     6541 accctcggta gcgcaaaata cctcgatatg gcacagcaat atcagcacct cggcccgctg
     6601 tatgaagtgc cggaaggtct gcgtaataaa gcgcgtcata acgaaccgta ctatcctccg
     6661 gttgagccag cccgtccggt tggcgacctg aaaacggctt aaggagtcac aatggaacag
     6721 gttgtcattg tcgatgcaat tcgcaccccg atgggccgtt cgaagggcgg tgcttttcgt
     6781 aacgtgcgtg cagaagatct ctccgctcat ttaatgcgta gcctgctggc gcgtaacccg
     6841 gcgctggaag cggcggccct cgacgatatt tactggggtt gtgtgcagca gacgctggag
     6901 cagggtttta atatcgcccg taacgcggcg ctgctggcag aagtgccgca ctctgtcccg
     6961 gcggttaccg tcaatcgctt gtgtggttca tccatgcagg cactgcatga cgcagcacga
     7021 atgatcatga ctggcgatgc gcaggcaggt ctggttggcg gcgtggagca tatgggccat
     7081 gtgccgatga gtcacggcgt cgattttcac cccggcctga gccgcaatgt cgccaaagcg
     7141 gcgggcatga tgggcttaac ggcagaaatg ctggcgcgta tgcacggtat cagccgtgaa
     7201 atgcaggatg cctttgccgc gcggtcacac gcccgcgcct gggccgccac gcagtcgggc
     7261 gcatttaaaa atgaaatcat cccgaccggt ggtcacgatg ccgacggcgt cctgaagcag
     7321 tttaattacg acgaagtgat tcgcccggaa accaccgtgg aagccctcgc cacgctgcgt
     7381 ccggcgtttg atccagtaag cggtacggta acggcgggta catcttctgc actttctgat
     7441 ggtgccgcgg ccatgctggt gatgagtgaa agccgcgccc gtgaattagg tcttaagccg
     7501 cgcgcccgtg tgcgttcgat ggcggtcgtt ggttgtgatc cgtcgattat gggttacggc
     7561 ccggttcctg cctcgaagct ggcgctgaaa aaagcggggc tttctgccag cgatatcggc
     7621 gtgtttgaga tgaacgaagc ctttgccgcg cagatcctgc cgtgcattaa agatctcggg
     7681 ctaatggagc agattgacga gaagatcaac ctcaatggtg gtgcgatcgc gctgggtcat
     7741 ccgctgggtt gttccggtgc gcgtatcagc accacgttgc tgaatctgat ggaacacaaa
     7801 gacgttcagt ttggtctggc gacgatgtgt atcggtctgg gtcagggtat tgcgacggtg
     7861 tttgagcggg tttaaccact tgccggatgc ggcgtgaacg ccttatccgg tctaccgatc
     7921 ctgcaccaat gtaggcctga taagacgcgc cagcgtcgca tcaggcatcg tgcaccgatt
     7981 gccggatgcg acatagatct aaatgccact gaataaagtt ttagttgccg ttcttcccgc
     8041 ctgtcagggg cgggttttta tatctcagat aaatgcaaac gcatcgccaa acagacgatc
     8101 ttcccgcgca ttacgctcac tgcaaaacag gtcacgagca attttcgcca tctcaaaacg
     8161 tccggcaata tagatatcat gctctgccag cgtaccatga tcctgcaaga ccgccgttaa
     8221 cacggtgcca gtacgcccac gccagcccgc ttccggttgt tcaaccaccg gcaccacttg
     8281 cagaccagga tgcttcagcg aaagcgcctc aagctcgcag agatcataca gatgttgctc
     8341 ttcacgccca ccccagtaaa tggtgatatc gcgattggga ttacgcgcca acgccgtcaa
     8401 taaaatcgag cgcgcatagg agaacccggt gccgcccgca atcaaaatca tcggacgctc
     8461 ttcatcatcg cgcagccacg cttctccgtg gggaatatcg accacgattt gatgatcttt
     8521 gaggatgcgg tccatgactg ctttcgcgta aaggttgatt tcagaagcgc caatatgcag
     8581 ctcgataaac cctttttcat ccggcgtcga agccattgaa aacggacgtt tgtcgcgctc
     8641 atccatcact accatcaaat actgaccagc acgaaaagaa aaggccgcgt ctggtacgat
     8701 tcggacacgg tatacagtat ccgtgatagc ttctaccgag gtcactttac agcttaaggt
     8761 tgtcatgcgc tttctctgtc ggatcaaata aatagggcaa aactaacgcg catcaggcgc
     8821 ttttaccgtt gttaaaaata gccagttcat cccagatggc gtcaatatgc gcgacgacat
     8881 caggatcttt tttgatggga cgtccccatt cacgctgggt ttcccccggc catttattcg
     8941 tggcatccag ccccattttt gaacccagcc cggagacagg cgaggcaaaa tccagataat
     9001 caataggcgt attttctacc agaacagtat cccgcgccgg gtccatacgg gtggtaatcg
     9061 cccaaatcac atcgttccag tcgcgtgcgt tgacgtcatc atcgcaaacg atcacaaatt
     9121 tagtgtacat aaactggcgt aagaacgacc agacgcccat catgacgcgc ttcgcgtgtc
     9181 cggcgtactg ttttttgatt gtcactaccg ccagacgata agaacagcct tccggtggca
     9241 ggtaaaaatc gacaatttcc gggaactgtt tttgcagaat cggcacaaac acttcgttca
     9301 gtgcgacacc cagcaccgcg ggttcatccg gcggacgacc agtataggtg gagtggtaaa
     9361 tcgcatcttc acgctgggta atatgcgtca cggtaaatac cgggaaacta tcgacttcgt
     9421 tatagtaacc ggtgtggtcg ccatacggcc cttccggcgc agtttcgcct tgttcgatat
     9481 acccttccag cacaatctcc gcactggcgg gcacttcaag gtcattggag atacacttca
     9541 ccacttcggt cttggtaccg cgcagcaatc cggcaaacgc atactctgaa agtgtatccg
     9601 gaacgggggt gactgcaccg agaatcgtgg cgggatcggc acccagcgcc acagaaaccg
     9661 ggaaacgttc gcccggatgc gccgcacacc actcctgata atccagcgcg ccgccgcgat
     9721 gcgacagcca gcgcataatc agtttgtttt taccaatcag ctgctggcga taaatgccca
     9781 gattctgccg ctctttatgc gggccgcgcg ttacggtcag cccccaggta atcagcggcg
     9841 cggcatcttc cggccagcag gtcataatgg gaatgcgatt gagatcgacg tcatcgccag
     9901 agacgatttt ttgttggcag ggcgcaccac gcagccgctt tgtcggcatg ttcaatactt
     9961 gcttaaactg cggcagttta tcaaacaggt cgcggaaacc ttttggcggc tcgggctctt
    10021 tcagaaacgc caataattta ccaacttcac gcagcgccga aacatcttcc tgccccatgc
    10081 ccatcgccac gcgctttggc gtaccgaaca ggttgcacag caccggcatt gagtagcctt
    10141 tagggttttc gaacaacagc gcaggcccac cggcacgcaa agtgcggtca gcaatttcag
    10201 tgatttccag atgcggatcc accgggagcg tgatacgttt tagctcaccc tgctgttcaa
    10261 gcagcgtcaa gaagtcgcgt aaatcgttat atttcatggc gtccattgta gcctcttaat
    10321 ctacgcccat tatacggcgt tcatctttgc gatgctgtaa atttgttaaa ttagcgtgaa
    10381 ctctgacggt ataacgcaaa ccggggaata taattaactt agcgtaaagc ttttgctatc
    10441 cttgcgcccc gattaaacgg ataagagtca ttatgcaatc ttggtattta ctgtactgca
    10501 agcgcgggca acttcaacgt gcccaggaac accttgagag acaggctgtg aattgcctgg
    10561 caccgatgat caccctggaa aaaatcgtgc gtggaaaacg tactgcagtc agtgagccat
    10621 tgtttcccaa ctacctgttt gtcgaatttg atccagaagt gattcatacc acgactatca
    10681 acgcgacccg cggtgtcagc cacttcgtgc gctttggcgc gtcgctagcg atagtcccat
    10741 cggcggtgat tcatcagcta tcggtatata aaccgaaaga cattgtcgat ccggcaaccc
    10801 cttatccggg agataaggtg attattaccg aaggcgcgtt cgaaggcttt caggccattt
    10861 tcaccgaacc cgatggtgag gctcgctcca tgctattgct taatcttatt aataaagaga
    10921 ttaagcacag tgtgaagaac accgagttcc gcaaactcta aaacgcaatc ccaaaccgtg
    10981 ttttgacatt agcatccgtg gtggcagcca gccatgcggc atcttctcca cgccagtgcg
    11041 caatacgttg caaaatatgg ggcagatggg ctggctcgtt gcgccgggat gatggctttg
    11101 gcgtgagatc gcgagggagc agatacggcg catcagtttc gatcagtaat ttttccgccg
    11161 gaatcaacgg caaaagttcc cgcagctcca gtccgcgtcg ttcatcgtaa acccaaccgg
    11221 taatgccgat ataaattcca tgcgccacgc acgcctgcat ctcttcgcgt gtgccggtaa
    11281 agcaatgaag aaccgcacca ggcagtttat ccagccacgg ctccagcaat gtcataaacc
    11341 gctcgtgggc atcgcgacag tgcataaata ccggcatgtt taaatctgcg gcaatgcgta
    11401 gctgggcaac aaaagcgcgt tcctgctctt ccggcgtcga aaagttgcgg ttaaagtcga
    11461 gaccacattc accaatcgcc accacttctg gctgcgcggc cagctcaata atcgcttctt
    11521 cagtcgcagc ttgccactgg ctgctgtcgt gaggatgtac gcccgccgtt gaccaacagg
    11581 acgaatactg acgcgccagc ttttgcgcct gctggctttc acgcaggtta gtgccggtga
    11641 tgagtagccc attaactccc gcgtcaaaag cgcgcgctac aacatcatca cggtctttcg
    11701 caaattgcga actggtcaaa ttaacgccga tatcaaacat cctgtactcc atatgacaac
    11761 cgccctgacg ggcggttgaa tttattcttc agttttttcg ctttctgctt cagcgtcgtt
    11821 ttcctcttcc cgatttcgcc ctttaccaac gtaaaagcgt gagaagaaga caccgatttc
    11881 aaacagacag tacatcggga tcgccaatag cgtttgcgag aagacatccg gcggcgtcag
    11941 caacatcccg acaacgaatg caccaaccag cacatacggg cgttttttgc gtaagtcttc
    12001 tggcgaggta atccccatcc agcacagcag cacaattgcc accggcactt caaaggagac
    12061 accaaacgcc ataaacagcg ccataacgaa gcttaaatag ctggcgatgt cggtggatac
    12121 ctgcacccct tccggcgcgg tattggcaag gaagccaaat gccagcggaa agaccacaaa
    12181 gtaggcgaat gccatgccga tataaaacag cagagagctg gaaaccagca gcggcaccac
    12241 caggcgacgt tcatgcttat acagcgctgg ggcgataaat gcccacacct gatagagaat
    12301 caccggcgct gacagaatca gcgacaccat aaaggtcagc ttgatcggcg taaagaacgg
    12361 cgaggccacg tcggtggcga tcatcgttga accttgcggc aactgcttga tcaatggcgc
    12421 ggataccagg tgatagatgt cattggcgaa atagaccaga cacaggaata tcacgatcac
    12481 cgcgataatg cagttcagca gacgcttacg cagctcaatt agatgcgtga taagcggttg
    12541 agtatcttct acagacatgt ttacggttta tcactcgacg aaggggaagg tgcagcggtt
    12601 ttcggttcag cgtccgcagc aggttttacc accggctctg gcgtggtttc tggcttctgt
    12661 tccggcgaac tggcctgcgt ttgtgcagcg gcaggcgtta cgccctcatg cgcagcttca
    12721 ttatctttca ccaccgggtt atggatggtg tgcgcttcat cgctcgcctt ttcaggatcg
    12781 tttgcaacgt aggaacgctt catcgactcc gcggcctggc gtagttcatc catcgacgct
    12841 ttcagttcgg gcgtcaggtt agtgaggctc gccttttcaa cctttttcag actgtcctga
    12901 aactcctgga gttttaactc ctgggtcagt tcgttctgca ccgttgtcgc cagtgaacgc
    12961 aacgcgcgaa tccagcccgc taccgttttt accgccacag gcagtcgttg cggccccaga
    13021 acgacgaggc cgatgatgaa caccaatagc agttcgctaa aaccgatatc aaacacggat
    13081 tacacctgct ctttatcgtg gcgcttcgcg tcttctgttt tagcctgttc ctgattcgta
    13141 tccgcctgct tatcggcgat agttttcgca gtaaaatcag catcctgact ggttttatcc
    13201 tgctttggtt catcatcgct cattgctttt ttaaagcctt tgatcgacgc accaagatcg
    13261 gaaccgatgg agccgagctt tttggtgcca aaaagcagta caacgatgac ggcaataatc
    13321 aataactgcc aaatactgat accacccata catgttcctc tgtggtagat gatgattaaa
    13381 caaggccgca ttatacgtta cacgacccgc cttgagcgat gaaaaaatca gcgtgttttg
    13441 cgccaaccga caaaccaggc gatcagacca cctgccatta accagccggg catcagcccc
    13501 cattcaggtc ggctgaccaa caagaatgtg ccacttaata ctaacgtagc gccaattccg
    13561 agaaaataac gcgattgtcc ctgacgtaca tgatttgact gaagctcgcg ggcaatctta
    13621 tcaacactgt gctgtaaata cttgccctgg cgcaaactgt cgtaaaccaa ttcaggcagt
    13681 tctggcattt tttcgaccca gaacggcgct ttttctttaa atgctctcac cagggcagga
    13741 ataccgacct gatctttaat ccacgactcc aggaaaggct tcgctgtttt ccataaatcg
    13801 agctgcggat aaagctggcg tcctacccct tcgacataga gcagggtttt ctggagtaac
    13861 accagttgcg gctgcacttc catattgaag cgacgcgccg tattaaacag atttaacagt
    13921 acatgtccaa acgaaatttc ggccagcggt ttctcaaaga taggttcaca gaccgtacga
    13981 atggcaaatt cgaactcttc aacgttggta tctggtggca cccagccaga atcgacgtgt
    14041 agctctgcca ctttgcgata gtcgcgatta aagaaggcga taaagttttc tgccagatag
    14101 cgtttatctt ctttgtttag cgagccaaca atcccgcaat caatgccgat atatttcggg
    14161 ttttccgggt gttcatagct tacgaaaatg ttgccagggt gcatatcggc atggaaaaag
    14221 ctgtcgcgaa agacctgagt gaagaacacc tgcacgccac gttccgccag caatttcatg
    14281 ttggtgccat ttttctccag cgtcgcaaca tcagacaccg gaatgccgta aatgcgctcc
    14341 atcaccatca tcccttcact acaatagtca gggtaaactt ccgggatgta gagcatcggg
    14401 ctgtcttcaa aatttcgccg aagctgaatg gcgttggcag attcccgcag caaattcagt
    14461 tcatcaatca aggtcttttc gtactcgcgc accacttcgg ttgggcgcag acggcgacca
    14521 tccggcagca aacgcggcac ccagcgagcc agacggtaga taagtttcag atccgcttta
    14581 ataaccggca aaatatccgg gcggatgact ttaatcacca cctctttacc attcgatttc
    14641 aatcgcgcgg tatgaacctg ggcgatagaa gcagaagcca gtggcttgat ttcaaaatcg
    14701 tcaaaccacg cttctaccgg caagccgccc attgcagctt caatctgctg cttcgccagc
    14761 ttgccatcaa acggagcaac tttgtcctgc aataacgcca gctgatcggc aatatgcggt
    14821 ggaaaaagat cgcggcgggt tgataacatt tgcccgaact tgatccaaac cggccccagt
    14881 tcttgcaggg ccagtcgtag tcgctcacct aaaagtttgt ctttatgccg atttggcatc
    14941 cagaataatg agtatcgcca tagccgtagc ggcagggtga tacgcatttt ggggatcagt
    15001 tcatcaagtc cgtagcttaa aaaagtgcga atgatgaaat ataggcgccg tacttcacct
    15061 ggcgtcattt agcctccagt ttttccagcc gtttggtcag ggcatcaaca gcacgctcga
    15121 cggcagccgt ctcttccgca aaccaggcca cttcaagcgg accgggtgcc atacgccact
    15181 cttcagtaat ggcttccgcc acataacgtt gctggcgctt aatgccgtga tgcaggaact
    15241 ttgcgcctcc gcgcatggct ttgctgattc cttcagcggc gatatcaccg gtataagggg
    15301 ccagcagttc cgcagggtcg aactctgcca gatctgccag cgcaacgaag ttttgcacca
    15361 cctgaatatc gccctgcact tccagctcac cactgcgaat cagtgcggta agctgctggc
    15421 gatcgcgaag tttcggcaac acactggcgt aggcgataac ggtgcagtca gcatcgcctg
    15481 cccattcgcc cagtacatca acctggcgtt cgctgaacac cagaatcaat gacgtcgaaa
    15541 agccttttac ctccacgcgc aatactttac ccagcagacg cgagcgggct gttttcagcg
    15601 cgggtgagcg atacaggaag gtgttgagca gactttcaat tcctgccgtc actaaaggtt
    15661 taaaaggcat ttccggtctc ctgtcagaac ttataaccac gatgcagcgc cacaacccct
    15721 gccgtcagat tgtagtagtc gacactttcg aatccggcat cctgcatcat ggctttcagg
    15781 gtatcctgat cgggatgcat acggatggat tctgccagat aacggtagct gtcggcgtcg
    15841 ttcgcgacca gtgagccaat acgcggcagc acatggaagg agtatgcatc ataggctttg
    15901 ctcagcggct cgataattgg cttcgagaac tcaagcacca gcaggcggcc gccgggtttc
    15961 agcacgcgat acattgaacg cagtgcttta tctttgtcgg tgacgttacg cagaccaaac
    16021 gaaatggtga tgcaatcaaa ggtgttatcc gggaacggca gcgcctcagc gttcgcctga
    16081 acatactcaa cgttgccaat cacaccgata ttacgcagct tctcgcgtcc cattttgagc
    16141 atggattcat tgatatcagc aaggaccact ttgccagttt ctccgaccag gcgggagaat
    16201 ttcgctgtca ggtcgccggt gccaccagcc agatccagca cggtctgccc acggcgtacg
    16261 ccgctgcaat caatcgtgaa tcgcttccac aaacgatgaa taccaaatga catcaaatca
    16321 ttcatgacat cgtattttga tgccacggaa tggaaaacgt gggcgaccat atccgctttt
    16381 tgttccttcg cgacggtctg aaaaccaaag tgcgtcgttt cttgtgactt atccaccatc
    16441 tcaatgcctg ctcatcaaaa aattgttcca gaagtgtaac agattgggcg tcgatgccct
    16501 agatttctac ccggcttaac tacccctaat gggctagcgc gactgctgat tatattcatc
    16561 atcgcgttga taagcttcat cattcggctg ctccggaacc gaccgaagtc gatactcttc
    16621 attctggctc accgcctgtt cagccaaatc cggattaatc tcgcgtttaa tttctactcc
    16681 taaaccgcga aacgcttctg cctgcgccag cacatttccg cgacctgaag agagtttttt
    16741 cattgcctgg cggtagttat cctgcgcttt gtcgagactt tgaccaatcg cggacatgtc
    16801 atcgatgaac aaacgcatct tgtcgtacag cttgctggca cgatcggcga tttgctgggc
    16861 gttgcggctt tgatgctcat aacgccacag gttggcaata gtacgcaacg ccaccagcag
    16921 cgtagtcggg ctaaccagca tgatattgtt tttcaacgct tcggtgatca gctccggctg
    16981 gcggtcaagc gccagtaaaa aagcgggttc aacgggaata aacatcagca cgtaatccag
    17041 agttcgcagc cccggcagct gttgataatc tttgcgtccc agcaaacgga tatggttacg
    17101 caccgacgcg atatgttcct gtagcgcgct ttcgcgggtg tagtcgtctt cggcgttaaa
    17161 atagcgttca taggcgacca gcgtcatttt ggcgtcgatc accacatctt ttccctgcgg
    17221 cagccgcacg atgacatccg gctgcatccg cgagcgggcg tcattttcga tgctgacctg
    17281 ggtttcatat tcataccctt cacgcagacc ggaagcctcc agcacccgcg tcaatactac
    17341 ctcgccccag ttgccctggg ttttattgtc gcctttcagc gcgcgcgtca ggttgatcgc
    17401 ttcctgggcc atttgcgcgt tgagttgctg gagattgcga atttcgtggg tcagggtatg
    17461 gcgttcttgt gcttctttac cgaagctgtc ctgaacctga cggcggaaac cgtccagttg
    17521 ttcacgtagc ggcgacaaca ggctgttcag actctgacgg ttttgctcat caacccggcg
    17581 attgctgtgc tcaaaaatac ggttggcgag gttttcaaac tgctcactga ggcgctgctc
    17641 gctgttaatc atctggcgaa ttttatcgtc agcatgttgc tgtgcggctt ccatccgcgt
    17701 ggttacttca cgcagatcgg cctccagaga ggtgttaata ctttgcaggc tgcgcacttc
    17761 gttattgagt aactcgcact ctgcacgcca gtgctcgctt tgggtaattt gttgttttgc
    17821 cgcgcttaac tccgcgacca tctcttcacg ttcagctaat tgctcggctt tttgctgcgc
    17881 atgttgataa ctggcaaaca gccagccaat tgccacaccc accaacgcaa taactgcgta
    17941 aaccatgatt gagaaatcca caacgcctcc tgacgcaaca acttacccgc acaaaataga
    18001 attgtgctgt acaaacgtcc agttggaaag gcatttcctg cgaggcgcta cgcaaaaata
    18061 caaaaggccg aacgcgtcgg ccttcagaca ggagaagaga attacagcag acgacgcgcc
    18121 gcttccacca cgattttcac cgcttggctt tcagtttgtt tcatcgtctc agcattcggg
    18181 atctcttgct gggtgcggtt aacgataaca cccgctacca taccggcacg caggccctga
    18241 cttgcacaca tggtcagcag ggttgcagat tccatttcat agttcattat gcccatcgcc
    18301 tgccactctt ccatagaacc tttaaagtga cgaactacgc gaccagagta agtatcgtaa
    18361 cgttcctgac ctgggtagaa ggtatcagaa gaagctgtca cgccaacgtg agttgtcgcg
    18421 ccaatggatt tcgcagcttc aaccagcgca gtcgtacatt cgaaatcagc gacagccggg
    18481 aattccagcg gtgcgaagtg caggctcgcg ccatccagac ggacagacgc cgtggtaacc
    18541 aggacatcac ccacattaat atgcggctga atagcgcccg ttgtaccgat acgcaggaag
    18601 gtgcgaatgc ccagctgtgc cagctcttca acagcaatag aggtagacgg gccgccgata
    18661 ccggtagagc agacgataac aggtttacca tccagctctg cacgccaggt agtgaattcg
    18721 cggtgagatg ccagcttaac cggcttatcc atcagcgcgg cgatcttttc cacacgatcc
    18781 gggtcgccag ggacgatggc aagcgtagcc ccttgtaaat cgtttttagt gaggccgaga
    18841 tgaaaaacat cagacttgga catatacaac tcctctgtga atcggtttag tcagaagaag
    18901 caaaaagaca ctttaccgaa gggtttaaca ttttttcgtg atactcatca ccatgacgca
    18961 aatgcgttgc ataaatttac ccaccaaaag tgattcaaat cacataaatc actcttcatt
    19021 ctgcaggact gcataaaaga tgagccgatt caggcaatgt gatttgttgc atgctgacta
    19081 tagttaacat tgcgcaacat ttttataagg gtacggagaa taccatggca acaacacaac
    19141 aatctggatt tgcacctgct gcatcgcctc tcgcttcgac catcgttcag accctggacg
    19201 acgcgattgt ggcgggcttc acctctatcc cttcacaagg ggataacatg cctgcttacc
    19261 atgccagacc aaagcaaagc gatggcccac tgccagtggt cattgtagtg caggaaattt
    19321 ttggcgtgca tgaacatatc cgcgacattt gtcgccgtct ggcgctggag gggtatctgg
    19381 ctatcgcacc tgaactttac ttccgcgaag gcgatccgaa tgattttgcc gatatcccca
    19441 cgttgcttag cggtctggta gcaaaagtgc ctgactcgca ggtgctggcc gatctcgatc
    19501 atgccgccag ttgggcttcc cgcaacggcg gcgatgttca tcgtttaatg atcaccggat
    19561 tctgctgggg tggacgtatc acctggctgt atgccgcgca taatccacag ctaaaagctg
    19621 cagtggcgtg gtacggcaaa ctgacgggcg acaagtcgct gaattcaccg aaacaacctg
    19681 ttgatatcgc aaccgatctt aacgcgccga ttctcggctt atatggcggt caggataaca
    19741 gcattccgca ggagagcgtt gaaaccatgc gccaggcgct gcgggctgct aatgcgaaag
    19801 cagagattat cgtgtacccg gatgccgggc atgcattcaa cgctgattat cgcccgagct
    19861 atcatgccga atctgcgaaa gatggctggc agcgtatgct ggaatggttt aagcagtatg
    19921 gagagaagcg gagttagttt cctcttagtc tatatttgag atggtgagta aacgagtgcg
    19981 ttttgctgcc atctctgtcc cccaaaaaat aatcagtatg aattgattgg agattacgac
    20041 tccgctattt gaccttaaga aagtcggcaa gtcatagtca taaatgagtc tcttcaatgg
    20101 aagacacctt agagatttac catcaactcc cattcacaaa caaacattaa ttattatcaa
    20161 cacattcaca ttaattataa atcatctcag accttcctga taaaaagata ttaaagtgaa
    20221 acattttgtt tttcacattt tagttcatca cttttaggcg taaataaagg taaaaaaaac
    20281 aacaatacta tttctctttc acaaaagaat taacaaaagg tttttcaagg atgagtgata
    20341 ttatttacct gaccataacc ggagaacaac aaggatgtat ttcctctcgt tgtggaacga
    20401 gcgcatcaat aggtaatcgc tggcagattg gccatgatga tgaaatcttc gcgttctcgc
    20461 tctctaatag tattacgaat acaggcaaag ggtcataatt gcatggcctt agtttctgta
    20521 aattgattga caaaagctcc ccgttactta ttaacgctat taacaataac gagcagttgt
    20581 tcatggagtt tgacttctat cggatcaata ggtttggtcg atgggagaaa tattacaaca
    20641 ttcaactccg cggcgcgctc ttatcagcaa tcaatcacct gtttactgag aacaatctgg
    20701 atacagaagc catcactgtc agttattaat atatactctc caggcatctg attgctaata
    20761 ctgaatttag ttattgggca tttcccgata actacaaccg tctgtttata ccacggccaa
    20821 aaactaaagc tggtaatggt ttcaaaacac ttaatagtaa agcggttggc agactgcttg
    20881 ctgctgtcgg catatacaat gggaatatcg aaggattcag ggaaacagca gacaagttgg
    20941 ggggagatgc acctgctggt tatgatcaat ttatggataa caaagggtta ctcatcgctg
    21001 gtgcttcaat agttgctgga gcagttctgg ggagaatgag actccctgat ttggaatcac
    21061 ttgagcattt cggagccaga ggagcagtat caggaagacc atttaatccc gagcttgccg
    21121 gtggtccaat agaaaacctg acgacagatg gcgtaacaat caacagggaa ggtatcgcta
    21181 ttgttgaaaa acatatagcc agatttggtc atgatcctgt aaatgaagtg atgatcaata
    21241 gactgaaaga cattgagaaa ggaaaaatcc ccccagaaca agtagatctt aacttctata
    21301 ctcatgagtg tcgggaatat cagcgttact gtaatcttgg ttgggaaaca ggacaacctg
    21361 atggtgacgc aggctatgca ctctggaatc atactggatt tgcccctata tttccagaca
    21421 tctgttatca cttaacccat tacaagcccg ctgcc
//
LOCUS       DAJTZL010000063        21419 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.063, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000063 DAJTZL010000000
VERSION     DAJTZL010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21419
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.063"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            270..1388
                     /locus_tag="OO544_002018"
     CDS             270..1388
                     /locus_tag="OO544_002018"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HCR6767622.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWADD"
     gene            1454..1702
                     /locus_tag="OO544_002019"
                     /pseudo
     CDS             1454..1702
                     /locus_tag="OO544_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
     gene            1767..2135
                     /locus_tag="OO544_002020"
     CDS             1767..2135
                     /locus_tag="OO544_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCR6767623.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            2229..2882
                     /gene="nfsB"
                     /locus_tag="OO544_002021"
     CDS             2229..2882
                     /gene="nfsB"
                     /locus_tag="OO544_002021"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HCR6767624.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNERKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            2990..4237
                     /gene="ybdG"
                     /locus_tag="OO544_002022"
     CDS             2990..4237
                     /gene="ybdG"
                     /locus_tag="OO544_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HCR6767625.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(4305..5681)
                     /gene="pheP"
                     /locus_tag="OO544_002023"
     CDS             complement(4305..5681)
                     /gene="pheP"
                     /locus_tag="OO544_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HCR6767626.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPDKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(5783..8926)
                     /gene="cusA"
                     /locus_tag="OO544_002024"
     CDS             complement(5783..8926)
                     /gene="cusA"
                     /locus_tag="OO544_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HCR6767627.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKARENGVPVYLRDVAKVQIGPEMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIASVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPSVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRK"
     gene            complement(8938..10161)
                     /gene="cusB"
                     /locus_tag="OO544_002025"
     CDS             complement(8938..10161)
                     /gene="cusB"
                     /locus_tag="OO544_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HCR6767628.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWFAKAEPPAEKTSTAERKILF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGTPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWLQLNTASEPMLLIPSQALID
                     TGNEQRVITVDADGRFVPKRVAVFQASQGVTALHSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATNAH"
     gene            complement(10177..10509)
                     /gene="cusF"
                     /locus_tag="OO544_002026"
     CDS             complement(10177..10509)
                     /gene="cusF"
                     /locus_tag="OO544_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HCR6767629.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEVQPQVISATGVV
                     KGIDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(10533..11915)
                     /gene="cusC"
                     /locus_tag="OO544_002027"
     CDS             complement(10533..11915)
                     /gene="cusC"
                     /locus_tag="OO544_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HCR6767630.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRTAMPVPQQFSLSQNGLVN
                     AADNYQNVGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGNTATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSGSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLNDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEISL
                     YTALGGGWQQ"
     gene            12072..12755
                     /gene="cusR"
                     /locus_tag="OO544_002028"
     CDS             12072..12755
                     /gene="cusR"
                     /locus_tag="OO544_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HCR6767631.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            12745..14193
                     /gene="cusS"
                     /locus_tag="OO544_002029"
     CDS             12745..14193
                     /gene="cusS"
                     /locus_tag="OO544_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HCR6767632.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFTRDAIPDKDAQGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTEPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDKCQVAGDPLMLRRALSNLLSNALRYTP
                     TRETIVVRCQTVDHQVQVTVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVIMLPERE"
     gene            14930..16831
                     /locus_tag="OO544_002030"
     CDS             14930..16831
                     /locus_tag="OO544_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HCR6767633.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTIWQGDEVQRRVKGVVTWFELGENDKNQMLYSMKVHPPLWRA
                     GLRQNFRIFQNEDIKSILGTMLQENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAYKSTDQSLVLCDTVRHLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFEQEGQHQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MDGWRNNAETARGMSRSPEIWPGRRIVLTGHPQANLNREWQVVASELHGEQPQAVPGR
                     QGAGTALENHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPADQDSSCWIRVAQAWAGTGFGHLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRPPGSLPGTKTQMTIRSKTYMGSGFNELKFDDATGREQVYIHAQKNMDTEVLND
                     RTTTVKHDHRETVKNDQTVTIQEGNRLLTVEKGHKITGVLKGSLSEDVFQDRGTIAGS
                     VHVDAVNNGGEGDGIQAYTAIKEILLAVEESKIALTPDSIQLQVGESTVIRLSKDGIT
                     IVGGSVFIN"
     gene            16859..17320
                     /locus_tag="OO544_002031"
     CDS             16859..17320
                     /locus_tag="OO544_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
                     /protein_id="HCR6767634.1"
                     /translation="MQFTFNEGHIQLPSQWQDQSMQVLVSTDNSGINLVITREAVPQG
                     TLTPELYQETLALYQGKLDGYTEHACREITLAEAPAWLLDYSWNGPEDEGNQGRISQI
                     AVFQRRGDTLLTFTFSTSLSLKNSQKTMLLEVIKSFTPLPPENDIQKDQPR"
     gene            17340..21419
                     /locus_tag="OO544_002032"
                     /pseudo
     CDS             17340..21419
                     /locus_tag="OO544_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616597.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
BASE COUNT         5166 a         5758 c         5640 g         4855 t
ORIGIN      
        1 caaaatggct gtttatttat acagtaaatc ctgtttcgga cttctcactc ctcatttaat
       61 caactgaagc tgagcccgtt ttttctgcat gcctcaatcg tcaaaattct tcgccatcag
      121 ccagctctgc cataacttgc cacattgaaa aagtagccaa attgcttcag tcaccatcat
      181 caaaaaaaga aacattccag aacttccgca tctcgcccag ccgctccgac ttctatactg
      241 aatagaaaac gccaacataa gagaaaccta tgccattacc cgattttcat gtttctgaac
      301 cttttaccct cggtattgaa ctggaaatgc aggtggttaa tccgccgggc tatgacttaa
      361 gccaggactc ttcaatgctg attgacgcgg ttaaaaataa gatcacggcc ggagaggtaa
      421 agcacgatat caccgaaagt atgctggagc tggcgacgga tgtttgccgt gatatcaacc
      481 aggctgccgg gcaattttca gcgatgcaga aagtcgtatt gcaggcagcc gcagaccatc
      541 atctggaaat ttgcggcggt ggcacacacc cgtttcagaa atggcagcgt caggaggtat
      601 gcgataacga gcgctatcaa cgaacgctgg aaaactttgg ctatctcatc cagcaggcga
      661 ccgtttttgg tcagcatgtc catgttggct gcgccagtgg cgatgacgcc atttatttgc
      721 tgcacggctt gtcacggttt gtgccgcact ttatcgccct ttccgccgcg tcgccatata
      781 tgcaaggaac ggatacgcgt tttgcctcct cacgaccgaa tattttttcc gcctttcccg
      841 ataatggccc gatgccgtgg gtcagtaact ggcaacaatt tgaagccctg tttcgctgtt
      901 tgagttacac cacgatgatc gacagcatta aagatctgca ctgggatatt cgccccagtc
      961 ctcattttgg cacggtggag gttcgggtga tggatacccc gttaaccctt agccacgcgg
     1021 taaatatggc gggattaatt caggctaccg cccactggtt actgacagaa cgcccgttca
     1081 aacatcagga gaaagattac ctgctgtata aattcaaccg tttccaggcc tgtcgctatg
     1141 ggctggaagg cgtcattacc gatccgcaca ctggcgatcg tcgaccgcta acggaagaca
     1201 ccttgcgatt gctggaaaaa atcgcccctt ccgcacataa aattggtgca tcgagcgcga
     1261 ttgaggccct gcatcgccag gtcgtcagcg gtctgaatga agcgcagctg atgcgcgatt
     1321 tcgtcgccga tggtggctcg ctgattgggc tggtgaaaaa gcattgtgag atctgggccg
     1381 atgactaagt agggaattgc cttacagaag aatatccctg acaatggtgt tttaacttac
     1441 atttaacaat gttatgaaac accctttaga aaccttgact accgcagcag gcattttgct
     1501 gatggctttc ctctcttgcc tgctgctgcc cgcccccgca ctggggctga cgctggcaca
     1561 aaaactggtg accatgttcc atctgatgga tcttagctag ctttacactt tattgttttg
     1621 tctgtggttt ttagtgctgg gcgctattga gtattttgtt ctgcgcttta tctggcgacg
     1681 ctggttctcg ctggcggatt aaacgtcgat tatcgccgta cttcgcggca tactttgctt
     1741 attctccttc gcctaaagga acgtttatgg ataagcaatc actgcacgaa acggcgaaac
     1801 gcctggccct tgagttaccc tttgtcgagc tttgctggcc ttttggcccg gagttcgatg
     1861 tttttaaaat tggcggcaaa atttttatgc tgtcgtcgga gctgcgcggc gtccccttta
     1921 tcaatctgaa gtccgatcca caaaaatccc tgttaaatca gcagatatac ccgagcatta
     1981 agccagggta tcacatgaat aaaaagcact ggatttcggt gtatcccggc gaggaaatct
     2041 ccgaagcgtt acttcgcgat ctgatcaacg attcgtggaa tctggtggtt gatggtctgg
     2101 ctaaacgcga tcaaaaaaga gtgcgtccag gctaaagcgg aaatctatag cgcatttttc
     2161 tcgcttacca tttctcgttg aaccttgtaa tctgctggca cgcaaaatta ctttcacatg
     2221 gagtctttat ggatatcatt tctgtcgcct taaagcgtca ttccactaag gcatttgatg
     2281 ccagtaaaaa acttaccccg gaacaggccg agcagatcaa aactctcctg caatacagcc
     2341 catccagcac caactcccag ccgtggcatt ttattgttgc cagcacggaa gaaggtaaag
     2401 cgcgtgttgc caaatccgct gccggtaatt atgtgttcaa cgaacgtaaa atacttgatg
     2461 cctcgcacgt cgtggtgttc tgtgcaaaaa ccgcgatgga cgatgcctgg ctgaagctgg
     2521 ttgttgacca ggaagatgct gatggccgct ttgccacgcc ggaagcgaaa gccgcgaacg
     2581 ataaaggtcg caagttcttc gctgatatgc accgtaaaga tctgcatgat gatgcagagt
     2641 ggatggcaaa acaggtttat ctcaacgtcg gtaacttcct gctcggcgtg gcggctctgg
     2701 gtctggacgc ggtacccatc gaaggttttg acgccgccat cctcgatgca gaatttggtc
     2761 tgaaagagaa aggctacacc agtctggtgg ttgttccggt aggtcatcac agcgttgaag
     2821 attttaacgc tacgctgccg aaatctcgtc tgccgcaaaa catcacctta accgaagtgt
     2881 aattctctct tgccgggcat ctgcccggct atttcccctc agattctcct gatttgcata
     2941 accctgtttc agccgtcatc ataggctgct gttgtataaa ggagacgtta tgcaggattt
     3001 aatatcccag gttgaagatt tagcgggtat tgagatcgat cacaccacct cgatggtgat
     3061 gattttcggt attatttttc tgaccgccgt cgtggtgcat attattttgc attgggtggt
     3121 actgcggacc ttcgaaaaac gagccatcgc cagttcacgg ctttggttgc aaatcataac
     3181 ccagaataaa ctcttccacc gtttagcttt taccctgcag gggattatcg tcaatattca
     3241 ggcggtattc tggctgcaaa aaggcaccga agcggcagat attctgacta cctgcgcgca
     3301 gttgtggatc atgatgtatg cgctgctttc agtcttctcg ttgctggatg ttattttgaa
     3361 tctggcgcag aaattcccgg cggcatctca gttaccgctg aaagggatat ttcaggggat
     3421 taaactgatc ggcgcgatcc tggtcggcat tttgatgatc tcgctgctga ttggtcagtc
     3481 gccagcgatt ctgatcagcg gtcttggtgc aatggctgcc gtgctgatgt tggtatttaa
     3541 agatccgatt cttggtctgg tggccggtat tcagctttcc gcgaacgata tgctgaaact
     3601 gggcgactgg ctggagatgc cgaaatacgg cgcagatggc gcggtgatcg atattgggtt
     3661 aaccaccgtc aaagtgcgta actgggacaa taccattacc actattccca cctggtctct
     3721 ggtttctgac tcctttaaaa actggagcgg gatgtcagca tctggcgggc gacgtattaa
     3781 gcgcagtatc agtattgatg tcaccagtat tcgttttctt gatgaagacg aaatgcaacg
     3841 tctgaataaa gcgcatttgt taaagcctta tttaaccagc cgccatcagg aaattaatga
     3901 gtggaatcgc cagcaaggtt ctacggagtc ggtattaaac ctgcgccgaa tgaccaatat
     3961 tggaaccttt cgtgcctatc tgaacgaata tctgcgtaac catccgcgga ttcgtaaaga
     4021 tatgacctta atggtacgcc aactggctcc aggtgataac ggtttaccgc tcgagatcta
     4081 cgcgtttact aacaccgtgg tgtggctgga atatgaaagc attcaggctg atatattcga
     4141 tcacatattt gcgattgtcg aagagtttgg tctgcgactt catcagtcgc caaccggcaa
     4201 tgatattcgc tctctggcgg gtgcatttaa gcagtaatta aaaaaaccgc tctcatcgaa
     4261 tggatgagag cggacatcgt tttgtcggat gtagcgtgaa tgccttattt ccgacgcagc
     4321 gttttaaatg ccacaaacag gaatacaatc cacaccggca gcaggatcgc tgacaagcgc
     4381 atatcatcca tcgtgcacat cagcagcaaa atcatgccga ggaaggcaat gcagagatag
     4441 tttccgaacg gatagagcag cgccttaaac tgtgtttcac gcccctgacg tcgcatcgct
     4501 gcacgaaaac gcagatgcgc cagacagatc ataatccagt tcaacagcag cgttgctacc
     4561 accagcgcca tcagcagacc aaacgctttt tgcggcagca gatagttgat taacaccacc
     4621 agcgaagtga tcgctccgga aagcatcagc gagttaatcg gcacaccgcg acggctgacg
     4681 cgagtcaaaa acttcggcgc attaccctgc acagaaaggc caaacagcat gcggctgtta
     4741 gagtaaaccc cgctgttata cactgacagc gatgctacca gaatgacgaa gttcagcgca
     4801 gaagctacca cgttgctgtc gagattatgg aaaatcatca caaacgggct actgttggat
     4861 ttcacttcca cccacggata gagcgccagt aaaaccacca gtgaaccgat gtaaaacagc
     4921 aggatgcgat acaccacctg atttaccgct tttggaatgc ttttatccgg atcgcgcgct
     4981 tcagcggcag taatcccaat cagctccaga ccgccgaagg agaacataat taccgccagc
     5041 gacaaaatca gcccattcca gccggtggcg aagaaaccac cgtagcgcca gaggttgtcg
     5101 atactggctt tctcgccgcc gtgaccagaa aacagcagcc acaggccaaa gccgatcata
     5161 ccgatgattg ccagcacttt aatcaacgca aaccagaact cggtttcgcc atataagcgc
     5221 acgttcacca ggttaacggc gttgatgata ataaagaagg cggcagccca aatccacgtt
     5281 ggaacatccg ggaaccagta ctgcatatag atgcccgcag cggtcagctc tgccattccc
     5341 accagcacga acattaccca gtagttccag ccagagagga agcccgcaaa cggtccccag
     5401 tatttatagg caaagtgggc aaatgaaccg gataccggct cttcgaccac catctcgcca
     5461 agctggcgca taatcaggaa agcgatgatc ccggcgacgc cgtaacccag caatacagcc
     5521 ggacccgcca tctgaatcgc cggaccaatg ccaagaaaca gaccagtacc aattgcgcca
     5581 cccagcgcaa tcagttgaat atgacggtta tgtaatccgc gatgaagcgt cggctcttga
     5641 ttcgacgcag tatcttccga tacggttgac gcgtttttca cgcctttccc ctgtgtgtct
     5701 tttttgttga ggggcacctt ttaacattta gtgcccatcg tcgcaagaca caatccacac
     5761 ggttaaacgg ggtatcctgc ttttatttcc gtacccgatg tcggtgcagc cacatcagct
     5821 tatacgccgc cgggataata aacagcgaca gcaaaggtgc ggtgatcatg ccgccaatca
     5881 tcggtgcggc aatccggctc atcacctctg aaccagcccc cgttccccac agaatcggca
     5941 gcagaccggc gataatcacc gccaccgtca tcgctttcgg gcgcacgcgc agtaccgcgc
     6001 cgtgatacaa cgcctcatcc agcttctgct cactgaatgt ttgcggatta ttcaacgacg
     6061 gctcggcctc tatggcgtga cgtaaataca tcagcatcac cacgccaaat tcggcggcga
     6121 ccccggcaag ggcaataaaa ccggtgcccg ttgccacaga aagatgaaag cccatccacc
     6181 acagcaacca gatgccgccc accagcgcaa acggtacgct gctgataatc agcaacgctt
     6241 cgcccacccg acggaatgcc agatacaaca gcacgaagat gatcatcagt gtcatcggca
     6301 ccatgagttt cagcttatgg ttggcgcgct ccagtagctc gaactgcccg gagaatgcca
     6361 cgctggtgcc cggttttaac tgcacttttt cagctatcgc tttttgcaaa tcgtgaacca
     6421 ccgacaccat gtcacgatcg cgggcatcaa tataaatcca gctcgtcggg cgcgcattct
     6481 cggttttcag catcgacggt ccggtagaga ctttaacgtc ggccacgtct gccagggtga
     6541 tttgctgctt catcggcgtc aggatcggta gctggcgcag tgcctgcggg ctatcgcgcc
     6601 agctttgcgg ataacgcaga ttaatgggat aacgggcaat cccttccacc gtttcgccaa
     6661 ccatcgcccc gcccaccgca gaagtcacaa acaactgcac atccgccacc gtcataccgt
     6721 aacgcgcggc tttttcacgg ttaatctcaa cgttgatata gcgcccacct tccagacgct
     6781 cggctagagc agaagccacg cttggcaccg ttcgcgctac ttcttcaatt tgctcagcca
     6841 tcgcgtcgat atccgccagc acagtgccgg aaactttaat gccgatgggg cttttaatgc
     6901 cggttgagag catatcgata cggttacgaa ttggcggcac ccacagattc gccagccccg
     6961 gcagccgcac ggtgttatcc agttcctcaa tgattttgtc catcgtcatg ccaggccgcc
     7021 actgatcctg cggcttaagc tggatggtcg tttcaaccat ctcgagcgga gcagaatcgg
     7081 tggcggtttc cgctttcccg gttttgccaa atacccgcgc cacttcaggt acgctcataa
     7141 ttagcttgtc ggttttttgc agcatactcg ccgcctctgc tgcggaaatc cccggcagcg
     7201 tcgatggcat atacaacaag tcgccttcat tgatctgcgg taaaaattcc ccgccaactt
     7261 tattgagcgg ccagagaacc gtcagcaccg aaagcgctgc caccaacagc gtggttttcg
     7321 gccagtgcag tactttcagc aatagaggat gataaacacg aatcaaaaag cgattgagcg
     7381 ggttactgct ttccggcgga attttgccac ggatccagta ccccatcagg atagggatca
     7441 ctacgatcgc cagcagcgcc gcgcccgcca tcgcataggt tttagtgaac gccaacgggc
     7501 caaacagacg cccttcctgc ccttccaggg tgaagatcgg gataaacgac aacgtgataa
     7561 tcagcagact gataaatagc gccggaccca cttcaacaga tgcatcggtg atcacctgcc
     7621 agcgcgtttt attatccagc gtggcgtcag gatgctggtg ctgccactct tccagccgtt
     7681 tatgtgcatt ttcgatcatg acgatagcgg catcgaccat cgccccgacg gcaatcgcaa
     7741 tgccgcccaa cgacataata ttggcattca gtccctggaa gtgcatgaca ataaaagcaa
     7801 tacacaaccc cagcggcaac gaaataatcg ccaccagcgc cgagcgcaca tgccagagaa
     7861 acagcgcaca gactaccgcc accacgataa actcttccag caacttgccg ctgaggttgt
     7921 cgatggcgcg gtcaatgagc tggctgcgat cgtatgttgt aactatctcc acgccttccg
     7981 gcagactact tttcagcgtt tccagtttgt ccttcacgga ggcgatcact tctcgggcgt
     8041 ttttgccgga gcgcaggatc accaccccgc ccgccacttc gccttcgcca ttcagttcgg
     8101 caatgccccg gcgcatctcc gggccaatct ggaccttcgc aacatcacgc agataaacgg
     8161 gcacgccatt ttctctggct tttaaaacga tgtgattaaa gtcgtcgagc gtttgcagat
     8221 agccgctggc gcgcaccata tattccgctt ccgccagttc gatcgacgaa ccgcccgctt
     8281 cctggtttga agcatccagc gcgcttttta cttcggctag actgatgcca tattgcgcca
     8341 ggcgctgggg atcgataacc acctgatact ctttcaccac gccgcccacc gacgccactt
     8401 ccgcaacgtc agggatggtt tttagctcat atttgagaaa ccagtcctgc aatgagcgta
     8461 aatcggccag atcgtgctta ccgctgcgat ccaccagtgc atattcatag atccagccaa
     8521 cacccgtggc atctggtccc agctcggcgc tgactcccgc aggcagctta ccctgtacct
     8581 ggttgaggta ctccagcacc cgcgaacgcg cccagtacgg atcggtacca tcttcgaaaa
     8641 tgacatacac ataagagtct ccaaactgcg agaaaccgcg cacagtcttc gcgccaggca
     8701 ccgacaacat ggtggtggtt agcggataag tcacctgatt ttcaacgatt tgcggtgcct
     8761 gaccgggata gctggtttta ataatcacct gcacatcgga gagatccggc agcgcatcca
     8821 ctggcgtgtt aatgatggtc caggtgcccc agatgctcag aaacaacgcg cccatcagca
     8881 ccaaaaaacg gttcgccacc gagcgacgaa taatccattc aatcattggt tattccctca
     8941 atgcgcattg gtagcacttt cagagcgcat ccgctccagt gcgccagaaa tattggcttc
     9001 ggaatcaatc aggaacaggc cgctggaaac caccttttca ccttccgcca gaccagagtg
     9061 taacgcggtg acgccttgcg atgcctggaa aacggcaacg cgtttcggta caaagcgtcc
     9121 gtcggcatca acggtaatca cccgctgttc gttgccggta tcaatcagcg cctgtgacgg
     9181 aatgagcagc atcggttcgc tggcggtgtt gagttgcaac caggcgttca tgcccggttt
     9241 tagcgcctcg tcggcgttgt cgacttccag acgcagctgc agcgtgcggg tcgcggcatc
     9301 cacgccaggt agcagcgtcc atttgcggat ggtgagtgtt ttatccggtc gcgccggaac
     9361 ggtgagcgta aactgcgagg catctttcac cagccaggcg atagactccg ggatcgcagc
     9421 agtgacccac accgggtcca taccctgaat tttcgctacc acgttatctt tggcgatatt
     9481 cattcccgcg cgcagatcaa acgcggtgat cacgccatca atgggtgctt tgagcgtaaa
     9541 gcgagtctgg attttttgcg tggcgatcag gcggcgaata tccgcctccg gcattcccgc
     9601 cagtcgcagc cgctcaagaa tgccttcagt ctgggtcgcc gtaccgccgg tttcgcgcag
     9661 cagtaaatac tcactctgcg cttccaccca gtcaggaatg gtcaggtcga gaagcggtgt
     9721 gcccttttgt actttatcgc ccacggtaag cggatacacc ttgtcgataa acccggcagc
     9781 gcgagcctgc acaatggcat actgatactc gttgtaactg acattcgccg ggaaactctg
     9841 ggcaaaagtc agcggtccgc gcgtgacggt agccgttttc acccccagat tctgcgtctg
     9901 agtcgggtca atgcgcacac cagacgcaga actctcttca tcggcatatt tcggcaccag
     9961 atccatatcc ataaacggcg atttacctgg tttatcgaac cgcgtattgg ggtacatcgg
    10021 gtcgtaccag aataagattt tacgttctgc ggtcgacgtt ttttctgcgg gtggttcagc
    10081 ctttgcaaac caggtaaaac ctgccgcaga aataataccg cccgcgatca tgctgccgat
    10141 aataagcgcg atttttttca tctcattaaa cctgggttac tggctgactt taatatcctg
    10201 taataaagaa aggttgccct gctggacaaa attaaacgcc actttgtcgc cggttttaat
    10261 ttcactcatt ttcgtctgcg gggtgatggt aaagcgcata gtcatctccg gccagttcac
    10321 ggcagcaatc ggatcgtgat ggatggtgat ttttttgctt tccagatcaa taccctttac
    10381 cacgccagtg gcgctaataa cctgcggttg tacttcgctc atggtttcat gatgatgttc
    10441 gttagcctgg gcattaaagc caataacggt aaacagactg aacattgcga cttgcagtgc
    10501 ttttttcatt tattctctcc tggagttaaa aattattgct gccaaccgcc ccctaaggcg
    10561 gtatacaaag aaatttcgtt aacctgacgg gcataattca ggtcgagtaa agtttgtcgg
    10621 gttgcaaata aagaacgctc ggcatccagc acttccagat aacttaccgc gccgtgctga
    10681 tataatgccc gcgcccgttg caaagtaatt tgcagcgacg ccagataacg ctgctgggcg
    10741 ctgatttgat cgttcaggct ttgacgtaat gccagcgcat ctgccacttc tttaaaggcg
    10801 ttctggattt tctgttcata attcactacc gactgctgct ggcgaatttc ggcgatatcc
    10861 agattggcct ggttgcgtcc ggcattaaaa atggggatct caattttagg aataaaattc
    10921 cacatcccgc tgctagcgtt aaataatgaa gacagatcgc tgctggcggt tgatattccg
    10981 ctggtcaggc tgatagacgg gaaaaatgcc gcacgcgcag caccaatatt agcattagcc
    11041 gccattaacg cgtgttcagc ttccataata tccgggcgct gcaataagat ttgcgacgac
    11101 aagcccgccg gtaatttaac gctttgcagg ctgccgctgt ttactgtctg cgcttgcggc
    11161 agtttgccgt agcttcccaa taacagttgc aatgcattat tcgcctgcgc cagttccccc
    11221 tgacgtttag cgatgtcact gcgggtactt tctatcaccc cgcgagcctg ttccagcgcc
    11281 agaacattgc tgctaccggt caacagttgt ttttcgacaa acgcatatga ctgctgataa
    11341 ttacgcagcg tttcttcggc tatttgcaat tgcgcatacg ccagttgctg attgaaatag
    11401 ctttgcgcga cattagaaac cagcagaata tgcaccgcgc gctgagcttc ctcagtggct
    11461 aaataatttt gtcgctcggc ttcgctcatg ttctttaagc gaccgaaaaa atcgaggtca
    11521 aagctggcgt taaggccggt cgaaaactcc cgtgtcgtgg cggtattccc tttaagatta
    11581 ccgctccagc tgccgctgcc ctcgccattg agctgtgggt agcggtcggc atcggtcaga
    11641 cgatattgcg cccgtgcctc ctgcactttc agcgtcgcca tgcgcaaatc ccggttattc
    11701 accagcgcct cgctaatcag cgtcttcacc tgattatcaa caaaaaaggt gcgccagccc
    11761 acgttctggt agttatctgc tgcgttaacc aagccgttct ggctgagtga gaactgctgc
    11821 ggcacgggca ttgccgtacg ctgataatcc ggtgccagtg aacaaccggt tagcgcaagg
    11881 gccacacaaa atggcagaag tttacaagga gacataggct cataatttct ggtgatttta
    11941 taccgccaac tttactcgcc aggctctgat tttccggtga caggaaaatg acaaaattgt
    12001 cattttgcca ataagcgatt gccatctgat cccgctactc tagaattgcc cgggcaacat
    12061 gcggaggaaa aatgaaactg ttgattgtcg aagatgaaaa gaaaaccgga gaatacttga
    12121 ccaaagggtt aaccgaagcc ggttttgtgg tcgatttggc cgacaacggg ctgaatggct
    12181 accatctggc gatgaccggt gattatgacc tgataatcct cgatattatg ctgccggacg
    12241 tgaacggctg ggatatcgtg cgcatgttgc gctccgccaa taaagggatg ccgattctgt
    12301 tgcttaccgc gcttggcacc attgaacatc gcgtcaaggg gctggagttg ggggccgatg
    12361 actatctggt gaagccgttc gcttttgctg aactgctggc gcgggtgcgc actctcctgc
    12421 ggcgcggggc ggcggtgatt atcgaaagtc agtttcaggt tgccgatctg atggtcgatc
    12481 tcgtcagccg caaagtcacc cgcagcggca cgcgcatcac tctgaccagt aaagagttta
    12541 ctctgctgga gttttttctc cgccatcagg gcgaagtgct gccccgctcg cttatcgcct
    12601 cgcaggtgtg ggacatgaat ttcgacagcg acactaacgc cattgatgtg gcggtgaagc
    12661 ggctacgcgg caaaatcgac aacgactttg agccgaagct gattcagacc gtgcgcggcg
    12721 tgggctatat gcttgaggtg ccggatggtc agtaagccat ttcagcgccc gttttcgctg
    12781 gcaacccgtc tgaccttttt tatcagcctg gccaccatcg cggcgttttt cgcctttgca
    12841 tggatcatga tccactcagt gaaagtgcat tttgccgagc aggatattaa tgatttaaaa
    12901 gaaattagcg ccacccttga acgggtactt aatcatcctg acgagacgca agcccgacgc
    12961 ttaatgacgc tggaagatat cgtcagtggt tattccaacg tgttgatttc cctggcagat
    13021 agccacggca aaacggtgta tcactccccc ggtgcgccgg atatccgcga gtttacgcgt
    13081 gacgccatac ccgataaaga cgctcagggt ggcgaggtgt atctcctttc cggcccgacg
    13141 attatgatgc caggccacgg tcacgggcat atggaacaca gcaactggcg gatgattaac
    13201 ttgccggttg gcccgttggt ggacggcaaa ccgatttata cgctctacat cgcgctttcg
    13261 atcgattttc atcttcatta cataaatgat ttgatgaata aacttattat gaccgcatct
    13321 gtcatcagca tcctgatcgt ctttatcgtg ctattggcgg tacataaagg tcacgcgccg
    13381 atccgcagcg tcagccgtca aatccagaat atcacctcga aagatctcga cgttcgcctt
    13441 gacccgcaga ccgagcccat tgagctggaa cagctggtac tgtcgttcaa ccatatgatc
    13501 gagcgtattg aggatgtctt tacccgccag tccaatttct cagcggatat cgctcacgaa
    13561 attcgcacgc cgattaccaa tctcattacg caaacggaaa tcgccctcag ccagtctcgc
    13621 agccagaagg agctggaaga tgtgctctac tctaatctcg aagagctgac gcgaatggcg
    13681 aaaatggtca gcgatatgct gtttctcgct caggccgata acaaccagct aatccccgaa
    13741 aagaaaatgc tcaacctggc ggatgaagtc ggcaaagtgt tcgatttttt cgaggcgtta
    13801 gcggaagatc gcggcgtgga gttgcgattt gttggcgaca aatgtcaggt tgcgggcgat
    13861 ccgctgatgc tgcgtcgggc gttaagcaac ctgctttcta atgccctgcg ttatacgcca
    13921 accagagaga caattgtagt gcgctgtcag acggtcgatc accaggtgca agttaccgtc
    13981 gaaaaccccg gtacgcccat tgcgcccgag cacttaccgc gattgtttga ccgtttctac
    14041 cgcgttgacc cttcccgcca gcgaaaaggc gaaggtagcg gcattgggct ggcgatagtg
    14101 aaatcgattg ttgtcgcgca taaaggcacg gttgcagtaa cgtcagatgc gcgggggaca
    14161 aggtttgtga ttatgctgcc ggagagagag tgattattaa gttcaaattc agggcgtggt
    14221 gcatcacctc ctggtattgt tgatcaacaa attagagaaa aacttattaa acatatgaaa
    14281 tatcgaaggc ttttattttt caaagtttca cgagatgttt gtatatgaaa cttagttaaa
    14341 tctgtgtttc ttccaattgt agatcacaat gccctttcta aattttcatc gttataaaca
    14401 agccatctat catataaagc aatgaataat ttaatcgcga ttaatcatca ttaattgtta
    14461 tattattcac tcaatctatt tgtcaagtga atttgataaa ttcctaattt aaaatatcca
    14521 ctattgagcc agtatttaac aacgttaaga tgtgcaatgt gcagatcatg ttatcagggt
    14581 ggagcggtag catatatttc ttatgcaatc attcatttta ataatgttaa ttggattatt
    14641 tattgctgtg aataaaaata tattatgaat attggtttaa ctttgcgttt ttgttactcg
    14701 ttgtgatatg cgtacctgtc ctgaataacg ccaagaaaca agaggttgag cttgtgatta
    14761 tttttttgac tgaacagaca cccgtttctg taaagtaata tttaaacaaa attctcacat
    14821 aaccatagca ttttctgttc cttccctaat aacgtctgaa gtcgtcccgc ttatcacatt
    14881 atccgcagtt gtacggtacg gatcattatt cattttactg gaggttgcta tgtcaaccgg
    14941 attacgtttc acactggaag tggacggcct gccgccggac gcttttgcgg tggtttcctt
    15001 tcatctgaac cagtcactct cttcgctctt ttccctcgat ctctccctgg tcagccagca
    15061 gtttctctcc cttgaatttg cgcaggtgct ggacaaaatg gcctacctga cgatatggca
    15121 gggcgatgaa gtacagcgcc gggtgaaagg cgtggtgacc tggtttgaac tcggggagaa
    15181 cgacaaaaac cagatgctgt acagcatgaa ggtgcacccg ccgctgtggc gtgccggtct
    15241 gcgccagaac ttccgtatct tccagaacga ggacatcaaa agcatcctcg gcacgatgtt
    15301 gcaggaaaac ggggtgaccg aatggagtcc gctgttcagc gagccgcatc cttcccgtga
    15361 gttttgtgtc cagtacggtg agactgatta cgatttcctg tgccggatgg cggcggagga
    15421 aggcatcttc ttttatgagg agcatgctta caaaagtacc gaccagagcc tggtgctgtg
    15481 cgacacagtc cgccatctgc ccgaatcttt tgaaatccca tggaacccga acacccgtac
    15541 cgaggtgagc accctctgca tcagccagtt ccgctacagc gcacaaatcc gcccttcttc
    15601 cgtggtgacc aaagactaca cctttaaacg ccccggctgg gccggacgtt ttgaacagga
    15661 aggccagcac caggactacc agcgcacgca gtatgaagtg tatgactacc ccggacgttt
    15721 caagggtgct cacgggcaga actttgcccg ctggcagatg gacggctggc gaaacaatgc
    15781 agaaaccgcg cggggaatga gtcgctcgcc ggagatatgg ccgggacgac gaattgtgct
    15841 gacggggcat ccgcaggcga acctgaaccg ggaatggcag gtggtggcaa gtgaactgca
    15901 cggcgaacag ccacaggcgg tgccaggacg gcagggagcg gggacggcgc tggagaacca
    15961 ttttgcggtg atcccggcag acagaacatg gcgaccacag ccgttgctga aaccgctggt
    16021 cgacggcccg cagagcgctg tcgtgacagg accggcaggc gaggaaatct tctgcgacga
    16081 acatggtcgc gtgcgggtga agttcaactg ggaccgttat aacccggcag accaagacag
    16141 ttcgtgctgg atccgtgtgg cacaggcgtg ggcaggcacc ggttttggcc acctggcgat
    16201 accgcgtgtg ggtcaggaag tgattgtaga cttcctcaac ggcgatccgg accagccgat
    16261 cattatgggg cgcacctacc accaggaaaa ccgccccccc ggcagcctgc cgggaacaaa
    16321 gacgcagatg accatccgct ccaaaacgta catgggcagc ggatttaatg agctgaagtt
    16381 tgatgatgcg acagggagag aacaggtcta catccacgcg cagaagaaca tggataccga
    16441 agtgctcaac gaccgtacca ccaccgtaaa acacgatcac cgcgaaaccg taaaaaatga
    16501 ccagacggtc acgatccagg aaggtaaccg ccttcttacg gtggaaaaag gccacaagat
    16561 caccggagta ctgaaagggt ctttatctga ggatgtcttt caggacagag gcacgattgc
    16621 cggttcggtg catgttgacg ctgtaaacaa tggtggcgaa ggcgacggta tacaggctta
    16681 tacggcgatt aaggaaattt tgctggccgt ggaggaaagc aaaattgcgc tgacgccgga
    16741 tagcattcag ctacaggtcg gggaatcgac ggtaatcagg ctgtcgaagg atggcatcac
    16801 catcgtgggc ggttctgttt tcatcaactg attcactcag attatcaggg agtattttat
    16861 gcagttcact tttaatgaag gccacattca actgccgtct caatggcagg atcagtcaat
    16921 gcaggtcctg gtgtccacag ataacagtgg catcaacctg gtcattaccc gcgaagccgt
    16981 tccacaggga accctgacac cagagctgta ccaggaaacg ctggcgctgt accagggcaa
    17041 acttgatggg tacaccgaac atgcctgccg ggaaatcact ctggcagagg ccccggcctg
    17101 gctgctggat tattcctgga acggtccgga agatgagggg aatcaggggc ggattagcca
    17161 gatagccgta ttccagcggc gtggagatac gctgctgacc ttcactttca gcacctcctt
    17221 atccctgaag aacagtcaaa agacgatgct gctggaggtg ataaagagct tcaccccgtt
    17281 gccaccggag aatgacatcc aaaaggacca gccgcgctga cataaacgag ggataacaca
    17341 tgagtgaagg accaggcggg ccacagggag cgaccgcagg cggtacgctg gcaatgcgaa
    17401 tgctgtcaca gcaggcgatg gtcgccagcc agatgaaacg ggcagccaac gacaaagcca
    17461 ttgcacagat gttggcatca aaaaagtctg gcccccccgc cgccaggctg ggcgatgaaa
    17521 ttcagcacaa gagttttttg ggggcgctgg caggggccgt actgggggcg atagtgacca
    17581 tcgcagaagg ttgcctgatt atggccgcct gtgccaccgg cccttatgcg ctggttctgg
    17641 tacctgcgct gatgtatgcc agctataagg cgagtgatta tgtggaggag aaacagaacc
    17701 agcttgaatc atggataaac agcttttgtg acacggacgg cgccatcaat accggttctg
    17761 aaaacgtaaa aattaacggc gagctggccg cacgtgcagc ggtcaccctt ccccctcctc
    17821 ccccacctgg agcaatacct gaagtcccac agggggaacc ctcatggggt gatattgcca
    17881 ctgacctgct tgaatcggca gcggaaaaag cagtaccact ggcgaaggcc tgggggaacg
    17941 ctgttatcac cctgacggac agcaatgccg gttttatgga tcgcgtcagc gccggcgcat
    18001 cgcttctgtt tcccgccggt ccggtattaa tggagtttgc caccatggtg ggcgggcgtg
    18061 gcgaaatcaa aaaagatgtg gatttcccgg aagccggtga ggacacggcg ctctgcgaca
    18121 aggagaacaa accaccgagg atagcccagg gcagcagcaa cgtctttatc aacaatcagc
    18181 ctgccgcgcg caagggcgac aaactggagt gcagtgcggc gatcgtggga ggttcgccgg
    18241 acgtctttat tggcggtgag caggtcacat atctggatat ccagccggag ttcccgccgt
    18301 ggcagagaat gatcctgggg ggaataacga tagccagcta tcttctgccg ccagcgggac
    18361 tactgggaaa actgaagaat ctggcgagac tcggcaaact gggaaactgc tggggaaaag
    18421 cgggaagctg ctgggcgcaa agctcggcgc gttgctgggg aaaacaggta agtcgttaaa
    18481 aagtattgcc aataaagtca tcagatgggt aacagatcct gtcgatccgg taaccggcgc
    18541 atactgcgac gaacgtaccg acttcaccct gggccagacc ctccccctct ccttcacccg
    18601 tttccacagt tctgtactgc cgctgcatgg cctgaccggc gtgggctgga gcgactcctg
    18661 gagcgaatac gcctgggtgc gtgaacaggg aaaccgggtg gatatcatca gccagggagc
    18721 cacgctgaga tttgccttcg acggtgacag tgatacggcg gttaacccgt atcacgccca
    18781 gtacattctg cgccgccgcg atgattatct ggagctgttc gacagggatg cactgagcag
    18841 ccgcttcttt tatgacgcct ttccgggaat gcgtctgcgc cacccggtga ctgacgatac
    18901 cagcgatgac cgcctggcac acagccccaa tgaccggatg tacatgctgg gcgggatgag
    18961 cgacaccgcc agcaaccgca tcacgtttga gcgcgacagc cagtaccgga tcacgggtgt
    19021 cagtcacacc gacgggatcc ggcttaaact gacgtaccac gccagcggct acctgaaagc
    19081 cattcaccgc acggataacg acatacagac gctggcgacc tacgaacagg atgcgcgggg
    19141 gcggctgaca gaagcggatg cgcggctgga ctaccacctg ttttatgagt acgacgctgc
    19201 ggaccggatc atccgctggt ccgataacga ccagacgtgg agccgtttca cctacgatga
    19261 acagggccgg tgcgtgaatg tcaccggggc ggagggctat tacaacgcca cgctggacta
    19321 tggtgacggc tgcaccaccg tgacggacgg caagggcatt caccgttatt actatgatcc
    19381 tgacggcaat attctgcggg aagaagcgcc ggacggcagc accaccatgt atgaatggga
    19441 tgaattccat cacctgctgg cccgccactc ccctgccggg cgggtggaga aatttgaata
    19501 caacgccgca cacggtcagt taagccgtta cacggcggca gacggcgcgg agtggcagta
    19561 ccgctatgat gagcgcggcc tgctcagcaa catcaccgac cctgccgggc agacgtggac
    19621 acagcagtgt gatgaacgcg gcctgccggt aagtctggtg tcgccacagg gcgaagagac
    19681 ccggctggca tacaccgctc aggggctgct gtcgggaata ttccgccagg atgaacggcg
    19741 tctgggcata gagtacgacc accacaaccg gccggaaaca ctcaccgacg tgatgggccg
    19801 tgaacaccac accgaataca gcggtcacga cctgccagtg aagatgcgcg gccccggcgg
    19861 tcagtcagtg cggttacagt ggcagcagca ccataaactg agcggcattg aacgtgctgg
    19921 aaccggcgcg gaaggatttc gctatgaccg ccacggcaac ctgctggcct ggacggacgg
    19981 caacggtgtt gtctggacga tggaatacgg tccgttcgat tttccggtgg cgcgaacgga
    20041 cggtgaaggc caccgctggc agtaccgcta cgataaagac acgctgcaac tgacagaagt
    20101 catcaacccg cagggtgagt catatcttta tgttctggat aactgcggcc gggtgacgga
    20161 agagcgtgac tggggcggcg tggtctggcg ttaccgctat gacgccgatg gcctctgtac
    20221 cgccaaggtt aacggcctgg aggaaaccat cctctacagc cgggacgccg caggccgcct
    20281 ggcagaaatc atctcgccgg aaggcaaaac gcagtatgcc tatgacaaat ccggcaggct
    20341 gacgggtatc ttcagcccgg acggcacatc acagcgcacc ggctatgacg aacgcgggcg
    20401 ggtgaatgtc accactcagg gccgacgggc cattgaatac aactaccccg atgaacacac
    20461 cgtcatccgc tgtatcctgc caccggaaga tgaacgcgac agacaccccg gtgaatccct
    20521 gctgaaaacc acgtaccgct acaacgccgc cggagaactg acagaggtca ttctgccggg
    20581 ggatgagacg ctgacgttca gccgtgatga ggcgggacgt gaagtgctcc ggcacagtaa
    20641 ccggggtttt gcctgtgaac agggctggaa tgcagccggt cagcctgtca gtcagcgcgc
    20701 cggatttttt ccggcagaag ccacatggga cgggctggtt ccctcactgg tacgggagta
    20761 ccgttacgac agcgcgggta acgtgtcagg cgtcaccagc cgggaagatt acggacggga
    20821 aacacggcgg gagtatcggc tggaccggaa cggccaggtc acggcggtga cagcctcagg
    20881 caccgggctg ggctatggcg aaggcgatga gtcctatggc tatgacagct gcggctacct
    20941 gaaggcgcag tctgcgggca ggcaccggat aagtgaagag actgaccagt atgccggagg
    21001 ccaccggctg aaacaggccg gaaacacgca gtatgactat gacgccgcag gccggatggt
    21061 cagccgcacc agacaccgtg acggctaccg accggaaaca gagcggttcc ggtgggacag
    21121 ccgggaccag ctgaccgggt attgcagcgc acagggtgag ctgtgggaat accgccacga
    21181 cgccagcggc agacgaacag agaaacgctg cgaccggaag aaaatccgct tcacgtatct
    21241 gtgggacggc gacagtattg cggaaatccg ggaataccgc gatgataaac tgtacagcgt
    21301 aaggcacctg gtgtttaacg gctttgagct gataagccag cagttcagcc gggtacggca
    21361 ggcgcatccg tcggtggccc cgcagtgggt gacgcggacg aatcatgcgg tgagcgacc
//
LOCUS       DAJTZL010000064        21192 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.064, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000064 DAJTZL010000000
VERSION     DAJTZL010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21192
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.064"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..210)
                     /locus_tag="OO544_002033"
     CDS             complement(<1..210)
                     /locus_tag="OO544_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HCR6767635.1"
                     /translation="MSIVKEEHKATLRKWHEELQEKRGERASLRRSTTVNDVCLTDGF
                     RLFLKNRQIKWQDEPEWRITALALIA"
     gene            complement(207..1768)
                     /gene="casA"
                     /locus_tag="OO544_002034"
                     /pseudo
     CDS             complement(207..1768)
                     /gene="casA"
                     /locus_tag="OO544_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311641.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
     gene            complement(1866..4565)
                     /locus_tag="OO544_002035"
     CDS             complement(1866..4565)
                     /locus_tag="OO544_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HCR6767636.1"
                     /translation="MRKYPLSLLKDKNIVTFFDFWGKTRRGEKDGGDDYHLLCWHSLD
                     VAAMGYLMVKRNCFGLADYFRQLGISDKEQAAQFFAWLLCWHDIGKFARSFQQLYLPP
                     ELKIQEGARKNYEKISHSTLGYWLWNHYLSECQELLPSSSLSPRKLRRVIEMWMPVTT
                     GHHGWPPDRMDELDNFLPEDKAAARDFLLEIKPLFPLIEIPAFWDDDEGIELIKHLSW
                     YISATVVLADWTGSSTRFFPRVAHPMDIKGYWQKTLVQAQNALTVFPLKAKVAPFNGI
                     NTLFPFIENPTPLQQKVLDLDISQQGPQLFILEDVTGAGKTEAALILAHRLIAAGKAQ
                     GLFFGLPTMATANAMYDRLVKTWLAFYSPESRPSLVLAHSARTLMDRFNESLWSGDLV
                     GSEEPDEQTFSQGCAAWFADSNKKALLAEIGVGTLDQAMMAVMPFKHNNLRLLGLSNK
                     ILLADEIHACDAYMSCILEGLIERQARGGNSVILLSATLSQQQRDKLVAAFARGTEGL
                     QEAPFLEKDDYPWLTHVTKSDVNSHRVATRKDVERSVSVGWLHSEQECIARIESAVSQ
                     GKCIAWIRNSVDDAIKVHRQLLARGVIPASSLSLFHSRFAFSDRQRIETETLARFGKE
                     DSSQRAGKVLICTQVLEQSVDCDLDEMISDLAPVDLLIQRAGRLQRHIRDINGQLKRD
                     GKDERSPPELLILAPVWDDSPGDEWFGSAMRNSAFVYPDHGRIWLTQRVLREQGAIQM
                     PHAARLLIESVYGEDVVMPEGFARSEQEQVGKYYCDRAMAKKFVLNFRPGYAANINDY
                     LPEKLSTRLAEESVSLWLATCIDGVVKPYATGAHAWEMSVVRVRRSWWKKHRDEFSLL
                     EGEAFRLWCIEQRQDPEMANVILVNDDESCGYSATEGLIGKVG"
     gene            complement(4760..4912)
                     /locus_tag="OO544_002036"
     CDS             complement(4760..4912)
                     /locus_tag="OO544_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HCR6767637.1"
                     /translation="MLTKYALVAIIVLCFTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(5177..5911)
                     /gene="cysH"
                     /locus_tag="OO544_002037"
     CDS             complement(5177..5911)
                     /gene="cysH"
                     /locus_tag="OO544_002037"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HCR6767638.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIRPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMSEEETRFFGLKRECGLHEG"
     gene            complement(5986..7698)
                     /gene="cysI"
                     /locus_tag="OO544_002038"
     CDS             complement(5986..7698)
                     /gene="cysI"
                     /locus_tag="OO544_002038"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HCR6767639.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIITGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(7698..9497)
                     /gene="cysJ"
                     /locus_tag="OO544_002039"
     CDS             complement(7698..9497)
                     /gene="cysJ"
                     /locus_tag="OO544_002039"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HCR6767640.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     PQTPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            9816..10178
                     /gene="queD"
                     /locus_tag="OO544_002040"
     CDS             9816..10178
                     /gene="queD"
                     /locus_tag="OO544_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HCR6767641.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            10256..11527
                     /locus_tag="OO544_002041"
     CDS             10256..11527
                     /locus_tag="OO544_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR6767642.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGVTTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRPLIKNTESLEYGAHLEP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGWYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWRHLRRHGLWHLAGDVIRSLRCL"
     gene            11518..11778
                     /locus_tag="OO544_002042"
     CDS             11518..11778
                     /locus_tag="OO544_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HCR6767643.1"
                     /translation="MSVAHNLWRVADAPHIVPADSVERQMVQRLINACPAGLFSLTPE
                     GDLRVDYHGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            11795..12370
                     /locus_tag="OO544_002043"
     CDS             11795..12370
                     /locus_tag="OO544_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HCR6767644.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGGMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(12518..13378)
                     /locus_tag="OO544_002044"
     CDS             complement(12518..13378)
                     /locus_tag="OO544_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HCR6767645.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDALVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSVRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     TRDPLAEARRVLVVGQGGEADNQEIAMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFVVAINHDASAAVFSQADVGVVDDWKVVLEA
                     LVTNIHADCQ"
     gene            complement(13375..14154)
                     /locus_tag="OO544_002045"
     CDS             complement(13375..14154)
                     /locus_tag="OO544_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HCR6767646.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGKSGPDISLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQHQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            complement(14132..15541)
                     /locus_tag="OO544_002046"
     CDS             complement(14132..15541)
                     /locus_tag="OO544_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6767647.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     AALTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLRQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVDFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            complement(15563..17017)
                     /locus_tag="OO544_002047"
     CDS             complement(15563..17017)
                     /locus_tag="OO544_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCR6767648.1"
                     /translation="MSLSRAAIVDQLKEIVGADRVITDETVLKKNSIDRFRKFPDIHG
                     IYTLPIPAAVVKLGSTEQVSRVLNFMNAHKINGVPRTGASATEGGLETVVENSVVLDG
                     SAMNQIINIDIENMQATAQCGVPLEVLENALREKGYTTGHSPQSRPLAQMGGLVATRS
                     IGQFSTLYGAIEDMVVGLEAVLADGTVTRIKNVPRRAAGPDIRHIIIGNEGALCYITE
                     VTVKIFKFTPENNLFYGYILEDMKTDFNILREVMVEGYRPSIARLYDAEDGTQHFTHF
                     ADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETWFNNLNWGPDKVA
                     AERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDITMLGGHSSHSYQ
                     NGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWS
                     KLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            complement(17087..17903)
                     /locus_tag="OO544_002048"
                     /pseudo
     CDS             complement(17087..17903)
                     /locus_tag="OO544_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417254.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            18221..19498
                     /locus_tag="OO544_002049"
     CDS             18221..19498
                     /locus_tag="OO544_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6767649.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIFQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSASLKTVIIIYSVVYILLGILCWFFVSDNNNLRSANNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVIQILSIIGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     RGLYWACPGEARTPSYIMGTTVGICSVIGFLPDVFVYPIIGHWQDTLPAAEAYRNMWL
                     MGMAALGMVIVFTFLLFQKIRTADSAPAIASSK"
     gene            19525..21010
                     /locus_tag="OO544_002050"
                     /pseudo
     CDS             19525..21010
                     /locus_tag="OO544_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311659.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
BASE COUNT         5098 a         5613 c         5220 g         5261 t
ORIGIN      
        1 ggctatcagc gccagtgcgg tgatacgcca ctcaggttca tcctgccact ttatttgtct
       61 gttcttcaga aacaggcgaa atccatccgt cagacaaaca tcattaaccg tcgtactacg
      121 ccgcagactg gcacgttcgc cgcgtttctc ctgcaattcc tcgtgccatt tgcgcagcgt
      181 ggctttgtgc tcctctttta caatactcat tcagcagcct cctgcttttt ctccctggcg
      241 gctttagcac tttgcttctc cgccgatgtt gtaaaatatt tcttgcgcgc ggtcatgatg
      301 cgttccaaat caacgggctc ataaggattg gtgaatacac gctcgtcaaa atcctgacgc
      361 gcgaataacc aaatttcctt attccatttg ctgagtaatt catccgcatc ctgaccttct
      421 tcaatttggc gcactaacct caggaagcga tgctgagttt tgttccagaa gtcgatatcc
      481 acaaaactaa aatcacccct tgcacctttt ggatcggaga accatgcttc tttcaatgca
      541 ctccgtaaca gacttagaat ccgtgaagcc gtttgcgcag ccagccgcag tttcggtatc
      601 tggccttctt tttattgagc agcagcggga aatgatgttc gtaccaacag cgcgctttca
      661 tgttgtcgaa atcatagcca aatccccaca ggcccacttt tgcctgcttg agactgctgg
      721 cattaaagag tttcaccacc agcgcgggaa gttccgtatt gttttctgac ttacccgttt
      781 cgataaggcc taaccagtcg cgccagatta aaccgcccgg ttgtggtttg accgagtaaa
      841 actcgccgcc ctctttaagt agtacacggt aaggcgttaa gggatgctgc cacatggcat
      901 agttagcgcc gtaatttttg gtcgtcatca ggctcagaag cgcgtcgctc tgctcaccgc
      961 aaatatcgca gttgccgact gtggtggtat taaaatcaat acgaatacgg cgaggcattc
     1021 cccagtacgc ctggagttta ttgacctgct catcggttac caccgcaccg gccagttcgc
     1081 tgatgcgcgt cgggccaagc caggggaaaa ccagatcgtc aaattttttg ggtagcggta
     1141 aatcggcttc atcctgcggc atgacattga tccacagttt gcgccacaag ggagtttgtt
     1201 gattaccctg atattcctgt aattcaatca gagtggtcat cggcccaccg ccgcgtaaac
     1261 cggtgcggta gcctttgcca cctgacggcg cattcaactg tagggagaac agagctaacg
     1321 cagagcaatg tgggcacagg tattccgtta caccacgctt gataaagtgg tctttattaa
     1381 acttcgttgt ttgagcgccg ggaatctcag gcagtagcga agcgaccgga actttatctc
     1441 cggtgagcgc ctcgaaatcc tgtataaatg aaggggaatc tggcccaaac tggaaagcat
     1501 gttctaatga cagcaatgct tcccgtagct tttcagcttc cagtccgtct tcccagatat
     1561 catcccaacg acgatgatct tttggcgcga agctggtttg tagtagcccc agcaaaaact
     1621 gccataccgc accctggaga tctgcccgcg gcgcagaaat atcgacaaca ttttcatccg
     1681 ccagatcgac tggcgccagt ttgcctgtcg ttccgtcttt aaaacgaacg ggcaaccacg
     1741 gggttgtcag aagtgaaaac gagttcatac gcatcctctc gtaaaaaaga ccatcccagg
     1801 tcggttgtcc atctttccat ccttttcggt gtatcaacta ttcagagagt cctgtcgcag
     1861 acatctcaac caaccttgcc aatcaatcct tccgtagccg aatacccaca actttcgtca
     1921 tcgttgacta aaataacgtt tgccatctcc ggatcttgtc gctgttcaat gcaccacagc
     1981 ctgaacgcct ctccctccag taaagaaaac tcatcccgat gttttttcca ccagcttcgg
     2041 cgcactctga caacgctcat ttcccatgcg tgagcaccgg tggcataagg cttcaccaca
     2101 ccgtcaatac aggtagccag ccacagggaa acagactcct cagccagacg tgtcgacagt
     2161 ttttctggga ggtaatcgtt gatattggcg gcatagccgg gcctgaagtt cagcacaaac
     2221 tttttagcca ttgcgcgatc acagtaatat ttgcccactt gctcctgctc gctgcgggca
     2281 aatccttccg gcattaccac gtcctcaccg tagacggatt caataagaag ccgggctgcg
     2341 tgtggcatct gaatagcacc ttgctcacgc agcacacgct gcgtcagcca gattcgccca
     2401 tgatcgggat agacaaatgc actgttacgc atggcactgc caaaccattc gtcgccagga
     2461 gagtcgtccc agacgggagc cagaatcagc aattcagggg gggaacgctc gtcctttccg
     2521 tcacgcttta actgaccatt aatatcgcgg atatgccgct gtaatcgtcc tgctcgctga
     2581 atcagcaaat caacaggggc caggtcggag atcatttcgt ccaggtcaca atcgacactc
     2641 tgctctaata cctgagtaca aataaggact tttccggcac gctgtgagct atcttcttta
     2701 ccaaagcgtg ccagtgtctc cgtttcaatt cgctggcgat cgctaaaagc aaagcggcta
     2761 tgaaaaagtg aaaggctgga agcaggaatg acgccgcggg caagcagctg acgatgaacc
     2821 ttaatagcgt catcgacaga attccggatc caggcgatgc atttcccctg acttaccgcc
     2881 gattcgatac gcgcaataca ctcttgttca ctatgaagcc aacccacgct gacgctacgc
     2941 tcaacgtctt tgcgcgtcgc tacccggtgt gagttcacat cggatttcgt gacatgcgtc
     3001 agccaggggt aatcatcctt ttccaggaac ggagcttctt gcaggccctc tgtaccccgc
     3061 gcaaaggcag cgacgagttt gtcgcgctgc tgttgagata acgtggcaga aagcaaaata
     3121 acgctgtttc cgccacgcgc ctgccgctcg atcaaccctt caagaatgca cgacatgtaa
     3181 gcgtcacagg cgtggatctc atcagccagc aggattttgt tactcagccc cagaagccgc
     3241 agattattat gtttgaacgg catcaccgcc atcatcgcct gatccagcgt gccgacgcca
     3301 atttcagcca gcaacgcctt cttgttactg tcggcaaacc aggccgcaca tccctgactg
     3361 aacgtttgtt catccggttc ttctgacccg actaaatcac cagaccagag tgattcatta
     3421 aagcggtcca ttaatgtgcg ggcactatgt gccagcacca ggctggggcg ggattctggc
     3481 gaatagaaag caagccaggt tttgaccagc cgatcgtaca tggcattggc cgttgccatt
     3541 gttggcagac caaaaaacag cccctgagcc ttccccgcag ctatcaacct gtgcgccagg
     3601 ataagcgccg cttctgtttt acctgcgcca gtcacatctt ccagaataaa taactgcggt
     3661 ccttgctggc tgatatccag atccagcacc ttttgctgta acggtgtcgg attctcaata
     3721 aaaggaaaca gcgtattaat tccgttgaaa ggcgcgactt ttgcttttag aggaaagacg
     3781 gttaaggcgt tttgagcctg aactaaagtt ttctgccagt aacctttaat atccattggg
     3841 tgtgcgacgc gtggaaaaaa tcgcgttgac gaacccgtcc agtctgcgag cacgacggtt
     3901 gcagagatat accaggaaag gtgttttata agttcaatgc cctcatcatc atcccagaat
     3961 gcggggatct ctatgagcgg aaacagtggt ttgatttcaa ggagaaaatc tcgtgctgca
     4021 gctttgtctt caggcagaaa attatccaat tcatccatac gatcaggggg ccaaccatga
     4081 tgccctgtcg tcacgggcat ccacatttct atcactcgcc taagtttgcg cggagagagc
     4141 gaagatgaag gaagtaactc ctgacattca cttaaataat gattccacaa ccagtagccc
     4201 agcgttgaat gagagatttt ttcgtaattt tttctggcac cttcctgaat cttgagttca
     4261 gggggcaggt aaagttgttg aaaagagcgg gcaaattttc caatatcgtg ccagcacagc
     4321 aaccaggcga aaaattgagc cgcctgttcc ttgtcagaaa tccctaattg acgaaaataa
     4381 tcagccagcc cgaagcaatt tcttttaacc attaaatagc ccatcgcggc tacatccagc
     4441 gaatgccagc aaagaaggtg atagtcgtcg cccccgtctt tctcgccgcg tcgggttttt
     4501 ccccagaaat caaaaaaagt cacaatattt ttatccttca gtaaacttaa aggatattta
     4561 cgcatatatt aaaaattatc tgtgatatat atcattcaat aaaatattta cattatgaat
     4621 aatcaaataa ttttcatccg taatatatat gaataataaa cataggcata atattcacat
     4681 aaataatata aattaaaaaa gggtgcagga gaccaaacca tcacaattca gtacgcgggg
     4741 ttttaccccc gcgtggtggc tacttcttcg attcgtaagc gagaactgcc ttaaactcca
     4801 taccacgttc tctgatactc aactcacaca acgagtcgcc taccatcagc gtgaatccca
     4861 gtactgtaaa acacagcact atgattgcca caagggcata ttttgtcagc atgtcttgcc
     4921 tccctgcaaa gaagagacta acattcgaat tgtttgggtt cgaacgctgg cctcaggttg
     4981 atagaaatat cgcctggggc ttttgtccat ctggaacctc gcgaatgctt aacgccagac
     5041 agcctcaagc acccgacgcc attctatacc tgataattct tccagcggcg ttcacgttta
     5101 aaatcaggaa tactgctcgc aaaataaaaa cgcccccatc atttctgaca gaggcgttta
     5161 atttatccgg caatatttac ccttcgtgca atccacattc cctttttaag ccaaagaaac
     5221 gtgtttcttc ttccgacatg ccaggttccc atttacgggt tgtatgggta tcaccgaccg
     5281 ataaatatcc ttcatcccat aatgggtgat atttcaggcc atgtttttgc aggtactgat
     5341 aaatagttcg gttatcccag tcgataatcg gcagcacttt aaatacgcca cgctgaattg
     5401 ccagcaccgg taaattggcg cgactgccgg attgttcacg gcgcaggcca gcaaaccagg
     5461 tttgcgcgtt aagttctttc agagcccggt tcatcggttc gactttgttg atgtcattgt
     5521 acttttcaat gccttcaacg ccctgctccc acagttttcc gtagcgtgct tcctgccagg
     5581 ctgcgctttc ggtagcacgg tacactttca ggttgagctt gagtttgtcc gttaactcgt
     5641 caataaagcg gtaggtttcc gggaacaagt aacccgtatc ggtgaggatc accggaatat
     5701 ccgggcgaat ttgattcacc agatgcaggc tcaccgccgc ctgaatgcca aagctggaag
     5761 aaagtacata ttcaccgggc agattatcca gcgcccaggc tacgcggcct tcagcgtcca
     5821 gtttttccag ttcggcgtta gtttccgcca gcgccagaat acgatctacc ttcggcagtt
     5881 cgttcagggc gtttagatcg agtttggaca tagattcctc actgtttgcc ttgcctgatg
     5941 cgacgctcac gcgttcttat caggcctaca agaccgggct gatggttaat cccacaaatc
     6001 gcgcgccgga tcgagcaccg ggcgaatgat gcccgcacgc accgtaaagt cgccgaagcc
     6061 ttcacccgct tcgcgctctt tcgcccagcg ccctatcagt tcatcaagcg acgccaggat
     6121 ttccggctcg gtgatgtttt ctttatacat ccgtgggata cgtgtcccaa tgcggttgcc
     6181 gccaagatgc aggttgtagc gacccggcgc tttacccacc aggcccactt ccgccagcat
     6241 cgcgcgacca caaccgttcg ggcagcctgt tacacgcatc acgatatgct catcgctgac
     6301 accatgtttc gccattaaat tatcgatgtt gtcgataaaa gacggcagga aacgctctgc
     6361 ttccgccatc gccagcgggc aagtcgggaa tgacacgcag gccatcgagt tttcacgctg
     6421 cggcgtgacg gcattcatta acccgctctc tttggcgatc ttctcgatct tcgctttctc
     6481 gctttccggt acaccggtga tgatcagatt ctggttcgcc gtaatgcgga aatcgccttt
     6541 gtggatcttc gcgatctcca gcaggccggt tttcagcgga cgccccggat aatcaaggat
     6601 gcgaccattt tcgataaaca gcgtcaggtg ccagttatca tcaatgccct taacccagcc
     6661 aatacgatcg cctcgtccgg tgaactcata tggacggatc ggttcaaatt tgatccccgc
     6721 gcgacgctcc acttccgctt taaacgtctc aacccccacg cgctccagcg tgtatttggt
     6781 tttggcattt ttacgatcgg ttcggttacc ccagtcacgc tgagttgtca cgacggcttc
     6841 ggccaccgcc agcgtatgct ccagcggcag atagccaaac tcgctcgccg tgcgggcgta
     6901 ggttttcttg ttgccgtgtt cgatagaaag cccaccgccc accagcaggt tgaagcctac
     6961 cagcttgccg ttttcggcga ttgccacgaa gttcatgtcg ttggcatgca gatcgatatc
     7021 gttctgcggc gggatcacta ccgtggtttt gaacttacgc ggcaggtagg tctgaccaag
     7081 gatcggttct tcatcagtag tggcgacttt ttcctggtcg agccagatct ccgcatacgc
     7141 gcgggtacga ggcaacagat gctcagaaat cttcttcgcc cactcgtacg cttccgcgtg
     7201 cagctgcgac tcgtaagggt tcgaggtgca gagtacgtta cggttcatgt cgttagctgt
     7261 cgccagcgca tcaagaccga ccgagtgcag catctggtgc accggtttga cgttcttttt
     7321 cagaatgccg tggaactgaa acgtctggcg gttggtcagg cgaatgctgc catagatggt
     7381 gttttcaccg gcaaatttgt cgatcgcctg ccactgttta gtggtaatca ccccacccgg
     7441 cagacgacag cgaagcagca tcgcgtggcg cggctccagc ttctgttcag cacgttcggc
     7501 acggatgtcg cggtcatcct gctgatacat gccgtggaag cggatcagca ggaagttgtc
     7561 gcccttaaag ccgccggtca gaccgtcgtt taaatcttcc gcaatggtgc cgcgcaggta
     7621 gttgctttca agcttcatgc gctcggcgtc tgtcagtttt ccttcgacca ctaaaggccc
     7681 tggatgtttt tcgctcatta gtagacatct cgctgataac ggcgctctac gcgcagctca
     7741 cttaaaaatt catccgccgc ttcggtgtcc atgccaccaa attcggcaat cacttccaga
     7801 agtgcctgct caacgtcttt cgccatgcga ttagcgtcgc cgcagacata aatgtgggca
     7861 ccgtcattga tccagcgcca cagctccgcg ccctgttcgc gcagtttgtc ttgtacataa
     7921 actttttctt tttggtcgcg cgaccaggca agatcgatac gcgtcagcac gccctctttg
     7981 acgtagcgct gccactccac ctggtagagg aaatcttccg taaagtgcgg gttgccaaag
     8041 aacagccagt ttttacccgg cgcttcgtcg gcggcgcgtt gctgcataaa ggcacggaac
     8101 ggcgcgatgc cggtaccggg gccaatcata atcactggcg tttgcgggtt agcgggcagg
     8161 cggaaattgt cgttatgttc gataaatacg cggacttcgc cctcttcttc cacgcgatcc
     8221 gcgaggaagc tggaggcacc accggcacgg gcgcggcctt ccacgtcgta acgcaccaca
     8281 ccaacggtga cgtgtacttc gttctcgact tccgcctgcg aggaggcgat ggaatacagg
     8341 cgcggggtca gcgggcgcag caggttaatc agcgcttcgg catccagttg cgccggagag
     8401 aaacgcacca tgtcgacaat cggcgtggtc gcggcgtaat gctgtaactt cgctttatcg
     8461 cccaccagcg gcagcagcgt ttcgctgcgg gtaagcgtgg catagttctc aacaatgttg
     8521 gcggtgttga cggtcagttc gaagtgccac tgtagcgctt cgttcagagg caacgttttg
     8581 ccctcgacgg tgacaggttc atcgcctttc agccacagca gttcgacaag ttctttcacc
     8641 agtgccgggt cgttctgata ccagacaccc agcgcgtcac ccggctggta acgcaggcct
     8701 gaatcgccta agtcaatttc gatatgacga acgtcttttt cagagttacg cccggtaatt
     8761 ttctggttaa ccgaaaggct cgccaccagc ggcgcgtctt tgctgtacgg gctggtgtgg
     8821 atttcattta ccgcgccagt agcgacggat tgcgaaggtg ccgcgacagg cgcacgcgat
     8881 ttaagcgcat caaccacgcg ggcgcgccac tcgctggcag cagcctggta ttcaacatcg
     8941 gcatcgacac ggtcgagcag gcgttcacca cccagttccg ccagcttgct gtcgaaatct
     9001 ttcccggact ggcagaaaaa ttcataagag ctatcgccga ggctaaacac ggcaaacgcg
     9061 gtgttttcca gctttggcgc ttttttggag aacaggaact tatgcagcgc gacggcttct
     9121 tccggcggtt ccccttcccc ttgcgttgac gtcactacga tgagcagttt ttcgctggcg
     9181 atttgtttga atttatagtc gcccgcgttc accagcttaa cgttcagttt tgctgctaat
     9241 aaatcatcgc gtaatgcttc agcaacccgg cgcgcattgc cggtttgcga ggcggagata
     9301 atagttatac ccggcatttc tgcggctggc gctggcgtcg ctgcaagcgc agcaggctgc
     9361 tgattgagta cgccccagaa atagccagaa acccaggcaa gctgggtagg cgttaaatcg
     9421 gtcgtggccg cctgaaggcg tgccagttgc tccgggttca acggaagcaa cgcggaaggt
     9481 gggacctgtg tcgtcatgcg tcgttatgtt ccagtaagca aagctgtttc tgcgccctgt
     9541 cagcgcccat aaaacagaag agaagataag gttaacgggg tgaacggtgt ggattaaaga
     9601 cgggatagcg ataactaata accaaatcga ctaacctgtt ttagcaatag tctttaacaa
     9661 caaaatagat taaccaaccc aatgaaaaac aaatgaattt agccaatcat taagataaat
     9721 cagcgatttt gcgcaacaaa gtcgttttag ataatgcgaa aaaacagcct ttccggtact
     9781 ctacggcggt tttatcgtcc tgtagagaaa ttatgatgtc caccacgtta tttaaagatt
     9841 tcaccttcga agccgctcac cgcttaccac acgtcccgga agggcataaa tgtggtcgcc
     9901 tgcacgggca ttcctttatg gtgcgactgg aaattaccgg ggaagtcgat ccgcatacgg
     9961 gctggattat cgatttcgct gaactgaaag cggcgtttaa gccaacttat gagcgcctcg
    10021 atcaccatta tctcaatgat attccaggtc tggaaaaccc aaccagcgaa gttttagcaa
    10081 aatggatttg ggatcaggtt aaacccgttg tgccgctgtt aagtgcggtg atggtaaaag
    10141 aaacctgcac cgcagggtgt atctatcgcg gcgaatgata agagtgtgtc ggcggtcaat
    10201 ttcccttaag taacgctatg ttagggtgtt gtgttctgga tatctggggc atgacatgga
    10261 agacgactgc gacattatta ttattggtgc cggtattgca ggcaccgctt gcgcgttacg
    10321 ctgcgcgcga gcgggtttat ccgttttgtt actggaacgc gctgaaatcc ccggcagcaa
    10381 aaatctttcc ggcgggcggt tatataccca tgcactcgcg gaactcctcc cgcaatttca
    10441 tctgaccgcg cctcttgaac gacgcatcac tcacgaaagc ctttccctgt taacgcccga
    10501 tggcgtaacg acgttttcca gcttacagcc cggcggtgaa tcctggagtg tattacgtgc
    10561 acggttcgat ccgtggctgg ttgccgaagc cgaaaaagaa ggtgtcgaat gcatccccgg
    10621 agcgacggtg gatgcactgt atgaagaaaa cggcagagtc tgtggcgtta tttgtggtga
    10681 cgatattctc cgcgcccgtt atgtggtgct ggcagaaggt gccaacagcg tcctggctga
    10741 acgtcacggg ttagtgactc gtcctgctgg cgaagcgatg gcgttgggga tcaaagaagt
    10801 gctgtcgctg gaaacatccg ctattgaaga acgttttcat ctggagaata acgaaggcgc
    10861 agcgttgctg ttcagcggcg gaatctgtga tgacttaccc ggcggcgcat ttctttatac
    10921 taatcaacaa acgctctcgt tagggattgt ttgcccgctc tcttccctta cgcaaagtcg
    10981 tgttccggca agcgagctgc tgactcgctt taaagcgcat ccggcagtgc gcccgcttat
    11041 caaaaacacg gaatcactgg agtatggtgc gcatctggag ccagaaggtg gcttgcacag
    11101 tatgccggtg caatacgccg gtaacggctg gctgctggtg ggcgatgcgt tgcgcagttg
    11161 cgtcaatacc ggaatttccg tgcgcggcat ggatatggcg ctgactggcg cacaggcggc
    11221 ggcacaaacg ctaataagcg cctgccagca ccgcgagccg caaaatctgt ttgcgcttta
    11281 tcatcacaac gtagagcgca gcctgctatg ggatgttcta caacgttatc agcatgttcc
    11341 ggcgcttttg caacgccctg gctggtatcg ggcgtggcct gcgttaatgc aggatatttc
    11401 ccgcgattta tgggatcagg gtgataaacc tgttccaccg ctgcgccagt tattctggcg
    11461 tcatttacgt cgtcatggcc tgtggcatct ggcgggcgat gttatcagga gtctgcgatg
    11521 tctgtagccc ataatctctg gcgcgttgct gatgcgccgc acattgttcc ggctgactcc
    11581 gttgagcgtc agatggtaca acggttgatt aacgcctgtc cggcaggtct tttttcgctc
    11641 acaccggaag gtgacttacg cgttgattat cacggctgcc tggagtgtgg cacctgccgt
    11701 ttgctgtgcg acgaatccac actacaacag tggcgctatc cgccttccgg attcggcatc
    11761 acctaccgct ttggataaat aataaggata atttatgccc ctcttacacc tactccgcca
    11821 gaatccggtg attgctgccg ttaaagataa tgccagcctg caactggcta tcgattctga
    11881 atgccagttt atttccgtgt tgtacggcaa tatctgcacc atcagtaata tcgttaaaaa
    11941 gattaagaac gccgggaaat atgcttttat tcatgttgat ttgctggaag gcgcgtcaaa
    12001 taaagaagtc gttattcagt ttttgaaact ggtgaccgag gcggacggca ttatcagtac
    12061 caaagcctca atgttgaaag cggcaagagc ggaaggtttt ttctgtattc atcgcctgtt
    12121 tattgttgat tcaatttcgt ttcacaacat tgataagcaa gttgcgcaat cgaatccgga
    12181 ttgtattgag atcctgccag gcggtatgcc caaagtgctg ggctgggtga cagagaaaat
    12241 ccgccaaccg ctgattgccg gtgggctggt gtgcgatgaa gaagatgcgc gtaatgcgat
    12301 taacgcgggt gtcgtggcgc tttccaccac gaataccggg gtctggacgt tagcgaaaaa
    12361 attactttga cgggataatc gtaaccaatt gaatttggtt tgatttgtag gccgcacgcc
    12421 acatccgaca ttcagcgcct gatgcgacgc ttgacgcgtc ttatcaggcc tacaagtccc
    12481 caggccagaa ccgtaggcag gatcatccgg caacacgtta ttggcaatct gcgtgaatgt
    12541 ttgtcaccag tgcctccagc accactttcc agtcatcaac cacgccaaca tctgcctgtg
    12601 aaaataccgc agcgctggcg tcatgattaa tcgccaccac aaacttgcta ttgcgtacgc
    12661 ccgccattaa cgctgctgcg ccagaagcgc caacaacaat acacacctca ggtgccagca
    12721 gatgtccgga aataccaatc actttttcag catcaacgcc accgttcatc actcgcgccc
    12781 gactgtagcc cacttcggct cccagctttt ctgccagcat agcgatttcc tggttatctg
    12841 cctctccgcc ctgccccacc accagcaccc gtctggcctc agctaatggg tcgcgagtaa
    12901 catttttcag atcctctgtg ctaaccagcc agtccggaag cgcacccggg acaatgttca
    12961 gttgttgcat tccagagggc aacgtggcgt ttttcgctgc tcccgcctga cgcgccaggg
    13021 aaaggcatag cgggcgtttt tctgtttgca gcgtcgccgt tagcgcattt ccccagtggg
    13081 atttgcgcac gcttacggtt ggaatatcta acgaagtcac ctggcagatg cttgcaccat
    13141 gtaaacgcca ggccagccgg gtcgacagtt catcgccaaa cgttcccggc ggaaagagaa
    13201 caacgtccgc aggcgttcgc tgccactgtt ccaccaacgc atccagcacc tgttccgcca
    13261 ctacaggctg aggttcaatc tgccagtgcg ccagggtaca accgctgaaa tcctgcgccg
    13321 ccagccagct tgctattgcg gcattctcct gatttattgt cacaattgcg atattcatgg
    13381 ctgcatcctt tggcgtaaat agtcctgcca tagcttttga gctttttctg ctaccgtttg
    13441 cccatcaatc agcgtcgctc cgcgtcgttg ctctgctctt gccagttgca gacactgcat
    13501 cgctggcatt tcggcagcaa cagttttacg gataatttct gctttccccg cagccattcg
    13561 ctggcgcatt cccggtactg gcaaggccac ttcaccgcac tgacgcacgg caatcaccgc
    13621 aggcaagcga acccggcaac aacgcaaccc atgttcagta cgctgttcga gggtgataaa
    13681 cagcgcgtcg agagtgaaac gctccacctg ggtgaagcag ggccagccca gcatttccgc
    13741 cagcaaaaat ggcgtttgcc cattctgccc ttcgctgctt tggcagccgg taattatcag
    13801 atccagcgga ttctgatgct gccattcggc aatatggcga gcgacaaatt ccggcgcaaa
    13861 gcgcagatct gccgccgtct ccagcaatac agcttcctca aacccaagag ccatgagata
    13921 gcgcagccag tgcaatgccc gttcatcccc catgctcaac gcggttaaag acatcggcgt
    13981 accgtttttt ctctgcgcca acagcagcgc ggcagcggcc tgttcatcag caccgagtaa
    14041 acttcgcagt agcgaaatat ccggtccgct tttaccctga gccgccgcct gccactcttt
    14101 ttccgccagc attccggcat ccggttcggc tttaaacgct aacagaatgt tcattcgcac
    14161 ctcctacatt tcccgccgcc accagcggga gggctttagt ttctggtgcc catagccagg
    14221 tcacaacaaa atcaaccaac aacactgtcg ccaggagcaa taaggtgact tgcattcccc
    14281 actgcgccag cacccacggc agcaggccag tacttaccgc cgcgccaagt cgactcatgg
    14341 cggtggcaaa accgacgccc agcgagcgaa tgtcagtagg aaaactttcc gcaggcaaaa
    14401 tgcccaccag attactgact gccgaaatgg tggtgctgaa gagaacaaaa agcagcagcg
    14461 ttaatgaact gccggaaggc aaacaggcca tcactaccag cgttgccgcc agcagcaaaa
    14521 aacttcccag caaaaatttg cgatgtgcca gcaggtgcgt cagaactaat cccagcagcg
    14581 cgcccacaat taacaacgca ttaagcatca ggctggcagt cagcgcatct tccagaccaa
    14641 tagtctgggc gatagttggc agccaggtat aaatcacaaa ccatgggatt acgaggcaga
    14701 caaagaagac gctgttaaac gccgtgcgcc gccagtaacg cgaagagaac aaggttttga
    14761 tgtgtttatg ggtcgccgtt accacttcat cgcccagtaa aacatgggga ccaaaatagc
    14821 gatgcacgat agcgtgagct tctgcaaaac gcccctggcg cagtagccag cgtggtgatt
    14881 ctggcgttcc ccagcgtaat aacgtaatca acaacgcggg cagagctgcc gatgccagta
    14941 gccagcgcca ggcctccggg ttttcggaaa taaagtgatg tccggcaata cttgccagca
    15001 catagcctac ggtccacacc acgctgaatg cgcccagcaa aataccgcga tggcggcgcg
    15061 gggaaaattc agccagcaag gtgtgaccta ctgaataatc gcctcccaga ccaatgccaa
    15121 tcaaaatgcg cagtccaata agatgctctg gcgtggtggc aaaaaattgt aaaaacgaag
    15181 caagcgtaat cagcaaaaag ctgaaggtga agattttttg ccgaccaata tggtcggaga
    15241 tccacccaag aaccaggctg ccaaggaaca aaccaaggag cgccgagccg ccgatcattc
    15301 ccgccataaa cggcgtcagt tgcatggcgg gcgtaagctg aataatggcg taaccaatga
    15361 cgccgagaac ataaccgtcg gtcaggtgtg cgccgaaagt gagcgcagca atgcggcagt
    15421 gaaaacggtt aagcggtaaa tcatccattc gcaccggtga agtgttcatc tttacccctt
    15481 cattgtccgg taataagaac ggcttctcgc ctgagaagcc gaaaaggcaa cgccccgtca
    15541 tcgcggaacg ctgcctgaca cattattttt caatcggata gatagtaccc gtattcataa
    15601 tgccattagg atcgaactgc tttttcagcc cttccagcaa cgcccacgcg ctgccgtgtt
    15661 ccagtttgct ccagtgaacg cgatgtttac cgataccgtg gtggtgcacc atcgaaccgc
    15721 cgaggcgaat ggtttcttca cagatgatct tgttgagcgg attgtggtac ttgtcgattt
    15781 cctcttccgg cttacagtca acgacgttgt aatcgtagac gaagtacatg ttggtgccgt
    15841 tctgatagct atgagaggaa tgaccgccca gcatggtgat gtcgtcggcg tgcgggaact
    15901 cagtacgaat acggttaata acgctttcgt agatttcgtg gatgcagctc cagcagccgg
    15961 acacttcagt ggtaaagccc atgttgccgg ttttgaggat ctgcacacgt tcggcagcca
    16021 ctttatccgg tccccagttc aggttgttaa accaggtttc gatcagcttg ctgtccacgc
    16081 gctggcattg cgggtagcgg gcaacgattt ccgcaatccc ttcgcccgtc gccttcgcaa
    16141 tgcgagggtt accttcagcc ataaagatca gcacgcattt tccgtctgca aaatgggtga
    16201 agtgttgggt gccatcttca gcgtcataca aacgagcgat cgacggacga tacccttcca
    16261 ccatgacttc acgcaggatg ttgaagtcgg ttttcatgtc ttccaggata tagccgtaga
    16321 agaggttgtt ttccggggtg aatttaaaga ttttcactgt tacttcagtg atatagcaaa
    16381 gtgcaccttc gttaccgatg atgatgtggc gaatgtccgg gccagccgcg cggcgtggca
    16441 cgtttttaat gcgtgtgacg gtgccatcag ccaatactgc ttccagacca acgaccatat
    16501 cttcgattgc gccgtagagt gtggagaact gcccgatact gcgggttgct accaagccgc
    16561 ccatctgcgc cagcggcctt gactgcggag aatgccccgt ggtgtaacct ttttcacgca
    16621 acgcattttc cagcacctcc agcggaacgc cacattgcgc cgtcgcctgc atattctcaa
    16681 tatcaatatt aatgatttga ttcatggcgg agccgtcaag caccaccgag ttttccacaa
    16741 cggtttccag cccaccttca gtggcggaag cgccggtacg cggcacaccg ttaattttgt
    16801 gcgcattcat aaaattcagc acacgggata cttgctctgt ggaaccgagt tttacgaccg
    16861 ctgccggaat cggcaaagta taaatgccat gaatatccgg aaatttacga aaacggtcaa
    16921 tactgttctt ctttaatact gtttcatcgg taattacgcg atctgcacca acaatttcct
    16981 ttagttggtc gacaatcgct gcgcgagata aagacatagt aattccttcc tgataataaa
    17041 aatggaataa tcattaataa gagaaaacag aaaccaccgt tatggattag cgcactaaat
    17101 aaccaccatt gaccactaat aaatgcccat tgacataatt tgaagccgga cttgcgagga
    17161 atacagctgc gcccattaaa tcctgagtat cgccccaacg gtttgccgga atatgatcaa
    17221 gaacgcgctg attggtttct ggattactgc gtgtcgccag cgtaatatct gttgcataat
    17281 agccaggagc aataccattt acctgaatat tatattgacc tagttcatca caataagctt
    17341 tggtgaatcc ggcaagagca tgtttagtgg cagaatatgc aggtgaccat tgtccaccta
    17401 agtaagagaa caatgaacag atattaataa ttttgccgct tttctgcggg atcataattt
    17461 ttgcagcttc atagcttaac tcgaatgcgg cggtcaggtt cacatcaatc atcggatccc
    17521 agtcggcacg accgaagtcc agcaccttat tcagcttaca aataccggca ttgtttacca
    17581 gaatatcaac tgtaccgaaa cgctcacagc aagcagcgat aatcttctgc ggcgcgcctt
    17641 ctgcggtgat atccacctgc atgaagtcca cctcaacacc ctgtttttca atcatttcct
    17701 ttgtttcgcc gttatctttc aatcatttcc tttgtttcgc cgttatcttt gacgaaacta
    17761 ggaataaaga tatttgcgcc agctttggcc aacgccatgg caaatgcctg gcctaaaccg
    17821 ctattcccac cggtaacaat tgcggtttta cctttcagtg agaaaaaatc cattgagaac
    17881 gcattgagag attcgattga catagttagc tccaatttcc gtaaggcaaa aaaaagaaag
    17941 gtaacttccc ccgcgagagg gaagttacct ttctcattat ctctggtaac tggagataat
    18001 ttttagcata acggcataac tgctaatgtg atcgtaatca cagtgtgata ttcgtattgt
    18061 caaatagttt taaaaagcag aagcgtgatt actctcacac aacacctctg ctttccatgt
    18121 tatttcttat tgcaattact agagacctga gaaaagtaat ccacctgaaa gggcggctta
    18181 cttttctctt ttttttgtct attacaaatg ggaatacgat atgcaacaca actcatatcg
    18241 ccgttggata accctcgcga taattagttt tagcggcggc gttagttttg acctggctta
    18301 tttacgttat atttttcaaa ttcccatggc gaaatttatg ggattcagca ataccgagat
    18361 aggtttaata atgagtacct ttggtattgc ggctattatt ctttatgccc ccagcggcgt
    18421 tattgccgat aaattttcac accgcaaaat gattacttcc gcgatgatca ttaccggatt
    18481 actgggtctg ttaatggcaa cgtatccacc gctgtgggta atgctctgta ttcaggtcgc
    18541 ctttgcgata acgacgattt taatgctgtg gtcggtgtcg attaaagccg cctcgttgct
    18601 gggcgatcat agtgagcaag ggaaaattat gggctggatg gaagggctgc gcggcgtcgg
    18661 tgtaatgtcg ctggcggtgt ttaccatgtg ggtcttttct cgctttgcac cggatgacag
    18721 cgccagcctg aaaacagtca ttatcatcta cagtgtggtt tacatcttgt tggggattct
    18781 gtgctggttt tttgttagcg ataacaacaa cctgcgcagt gccaataacg aagaaaaaca
    18841 gtcattccag cttagcgaca tccttgccgt tttgcgtatc agcaccacct ggtattgcag
    18901 catggtgatt tttggtgtct tcacaatcta cgccattctg agttactcca ccaactatct
    18961 gaccgaaatg tacggcatgt cgctggtggc agcgagctac atggggattg tgatcaacaa
    19021 aatcttccgc gcgctgtgcg gcccacttgg aggcattatc accacctaca gtaaagtgaa
    19081 atcccctacc cgcgtgattc aaatcctttc catcatcggt ttgctggcgt tgacagccct
    19141 gctcgtcacg aactctaacc cccaatcggt cgcgatgggg attggcctga ttttactgct
    19201 gggattcacc tgttacgcct cacgcgggct gtactgggcc tgccccggcg aagcgagaac
    19261 accgtcttac attatgggca ccacggtagg tatttgttcg gtgattggat tcctgccgga
    19321 tgtcttcgtt tacccgatta tcggccactg gcaagacacc ctgcccgctg cagaagccta
    19381 ccgcaatatg tggctgatgg gcatggcggc gctcggcatg gtgattgtct ttaccttttt
    19441 gctgttccaa aaaattcgta ctgctgatag cgcccccgca atagctagca gcaagtaagc
    19501 caggaaaatt acaggagaaa aatcatgtcg aaaaaataca tcatagggat tgatggcgga
    19561 agtcagagca caaaagtggt gatgtacgat ctggaaggta atgtggtttg cgaaggtaaa
    19621 ggcttattac agccgatgca cacgcctgat gccgatactg cagaacatcc tgacgacgat
    19681 ttatgggcat cattatgttt tgcaggtcac gatttgatga gccagtttgc cgggaataaa
    19741 gaagatattg ttggcatcgg cctgggctcc atccgttgct gccgtgcgtt attgaaagcc
    19801 gacggcacgc cagctgcgcc gttgattagc tggcaggatg cacgcgttac acgcccttac
    19861 gaacatacga atcctgacgt ggcgtatgta acctcttttt cgggttatct gacgcatcgc
    19921 ttaaccggcg aatttaaaga taatatcgcc aactattttg gtcagtggcc ggtggattat
    19981 aagacctggg catggagcga agatgccgca gtcatggaga agtttaatat cccccgtcat
    20041 atgctgtttg atgtgcaaat gccaggcact gttcttgggc atatcacacc tcaagccgcg
    20101 ctggcgacgc atttcccggc aggactgccg gttgtttgta ccaccagtga taaaccggta
    20161 gaagctctgg gagccggatt actggatgat gaaactgcgg taatttctct tggcacttac
    20221 atcgcattga tgatgaacgg caaagccttg ccgaaagatc cggtggcgta ctggccgatt
    20281 atgtcttcta ttccgcaaac attgctgtat gaaggttacg gtattcgcaa aggcatgtgg
    20341 acggtgagct ggctgcgcga catgttaggc gagtcgttaa ttcaggatgc cagggcgcag
    20401 gatctttcac cggaagattt actcaacaag aaagcctcta gtgtgccacc tggctgtaat
    20461 gggctgatga cggtgctgga ctggctgacc aatccgtggg aaccgtacaa acgcgggatt
    20521 atgatcggct ttgattccag catggattac gcatggatat atcgttcgat attggaaagc
    20581 gtggcgctga cgctgaagaa caattacgac aatatgtgta atgaaatgaa tcactttgcg
    20641 aaacatgtaa tcattactgg cggcggttcg aacaggatct gtttatgcag atttttgccg
    20701 acgtgttcaa ccttccggca cgccgtaacg ccattaacgg ttgtgcaagc ctgggggcag
    20761 cgattaatac aggggtaggt ctggggctat acccggatta cgcaacggct gtcgataaaa
    20821 tggttcgcgt gaaagatatc tttataccga ttgagagcaa tgccaaacgc tacgacgcga
    20881 tgaataaagg cattttcaaa gacctaacca aacatactga tgtgatcctg aaaaaatcgt
    20941 atgaagtgat gcatggggaa ttggggaatt ggggaatgcg gattcgatcc agagctggtc
    21001 gaatgcgtga ataaggtagg ccgggcggaa aactgcccgg cctgaacata cctgaatggt
    21061 tatccccgct ggcgcgggga acataagttc gacaacttaa aaaatgataa caagtccggt
    21121 ttatccccgc tgatgcaggg aacataataa aaactttaac tagttcggtt tatccctgtt
    21181 tttacaagga at
//
LOCUS       DAJTZL010000065        21086 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.065, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000065 DAJTZL010000000
VERSION     DAJTZL010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21086
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.065"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..430)
                     /gene="matP"
                     /locus_tag="OO544_002051"
     CDS             complement(<1..430)
                     /gene="matP"
                     /locus_tag="OO544_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HCR6767650.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDL"
     gene            616..2376
                     /locus_tag="OO544_002052"
     CDS             616..2376
                     /locus_tag="OO544_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HCR6767651.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIANAARTLQSDAGQLVGGHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVTQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            2445..2963
                     /gene="fabA"
                     /locus_tag="OO544_002053"
     CDS             2445..2963
                     /gene="fabA"
                     /locus_tag="OO544_002053"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HCR6767652.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(3033..3200)
                     /gene="rmf"
                     /locus_tag="OO544_002054"
     CDS             complement(3033..3200)
                     /gene="rmf"
                     /locus_tag="OO544_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HCR6767653.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(3456..4019)
                     /gene="pqiC"
                     /locus_tag="OO544_002055"
     CDS             complement(3456..4019)
                     /gene="pqiC"
                     /locus_tag="OO544_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HCR6767654.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLASVWSQEAASIAQEIKRLP"
     gene            complement(4016..5656)
                     /gene="pqiB"
                     /locus_tag="OO544_002056"
     CDS             complement(4016..5656)
                     /gene="pqiB"
                     /locus_tag="OO544_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HCR6767655.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGLE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKVRLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            complement(5661..6914)
                     /gene="pqiA"
                     /locus_tag="OO544_002057"
     CDS             complement(5661..6914)
                     /gene="pqiA"
                     /locus_tag="OO544_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HCR6767656.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKATCPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSQQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            complement(7044..8951)
                     /locus_tag="OO544_002058"
     CDS             complement(7044..8951)
                     /locus_tag="OO544_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6767657.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQEPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLLEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLDQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKTEEAAAAKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            complement(8963..11071)
                     /gene="rlmKL"
                     /locus_tag="OO544_002059"
     CDS             complement(8963..11071)
                     /gene="rlmKL"
                     /locus_tag="OO544_002059"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HCR6767658.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRTGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWVQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHMALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            11315..12424
                     /gene="ycbX"
                     /locus_tag="OO544_002060"
     CDS             11315..12424
                     /gene="ycbX"
                     /locus_tag="OO544_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HCR6767659.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDRWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVMPLKKSAMGDDGTILCCSCVPKTALKLAR"
     gene            complement(12421..12963)
                     /gene="zapC"
                     /locus_tag="OO544_002061"
     CDS             complement(12421..12963)
                     /gene="zapC"
                     /locus_tag="OO544_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HCR6767660.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(13137..14147)
                     /gene="pyrD"
                     /locus_tag="OO544_002062"
     CDS             complement(13137..14147)
                     /gene="pyrD"
                     /locus_tag="OO544_002062"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HCR6767661.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            complement(14258..14995)
                     /locus_tag="OO544_002063"
     CDS             complement(14258..14995)
                     /locus_tag="OO544_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HCR6767662.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELRAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPAPLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTSWCQ
                     AIAPCHVAWRAINDYGGLSAKKEQNLP"
     gene            complement(14961..15476)
                     /locus_tag="OO544_002064"
     CDS             complement(14961..15476)
                     /locus_tag="OO544_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR6767663.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            complement(15484..16026)
                     /locus_tag="OO544_002065"
     CDS             complement(15484..16026)
                     /locus_tag="OO544_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6767664.1"
                     /translation="MKKETIFQCVILFFSILNIHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGDFNISDFQAANTVSTAADLNIDITGCAASITGADVLFSGEADTLTP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVLYFDLVYQ"
     gene            complement(16038..17108)
                     /locus_tag="OO544_002066"
     CDS             complement(16038..17108)
                     /locus_tag="OO544_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6767665.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYSGQCHTTQGNPYI
                     GVNFGVKTLEEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDAFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATTDPSYPQAIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPDDADSIARMNVYPVS
                     TTGVPPETGRFEATATVRINFD"
     gene            complement(17099..19698)
                     /locus_tag="OO544_002067"
                     /pseudo
     CDS             complement(17099..19698)
                     /locus_tag="OO544_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
     gene            complement(19723..20424)
                     /locus_tag="OO544_002068"
     CDS             complement(19723..20424)
                     /locus_tag="OO544_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCR6767666.1"
                     /translation="MKTCITKGIVTVSLTAILLSCSSAWAAGKGGIGLAATRLVYSEG
                     EEQISLGVRNTSPDVPYLIQSWVMTPDNKKSADFIITPPLFVLNPANENLLRIMYIGA
                     PLAKDRETLFFTSVRAVPSTTKREEGNTLKIATQSVIKLFWRPKGLAYPLGEAPAKLR
                     CTSSADMVTVSNPTPYFITLTDLKIGGKVVKNQMISPFDKYQFSLPKGAKNSSVTYRT
                     INDYGAETPQLNCKS"
     gene            complement(20507..21049)
                     /locus_tag="OO544_002069"
     CDS             complement(20507..21049)
                     /locus_tag="OO544_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6767667.1"
                     /translation="MKKSVLTAFITVVCATSSVMAADDNAITDGKVTFNGKVIAPACT
                     LVAATKDSVVTLPNVSATKLQTNGAVSGVKTDVPIALEGCDVTVTKNATFTFSGTADG
                     VQPTAFANQATTDAATNVALQMYLPDGSTSVTPGTETSNIQLADSAEQTVTFKVDYIA
                     TGKATSGNVNAVTNFHINYY"
BASE COUNT         5213 a         5547 c         4922 g         5404 t
ORIGIN      
        1 gcagatcctg cttcagagaa gacattttat tcgcgtattt ctctttgttt tccgcatctt
       61 caatcagctg aacaatcgtt tcagaaagcg ttttaccgcg acgctgcgca agaccagcca
      121 gacgttgcca gacaataaat tccagatcga tcgatttttt gcgcgtatgc tggtgttccg
      181 cattaaaatg gcgctttcgt cttgcccgaa tggtttgctt catgcgattg accagttccg
      241 gattcatatg cttgtcaatc cagccattta ccagcacggg ttcattttcc agcgagagca
      301 acacatcgac ggcttcctgg gcggcactgg cttctatgta acgggtgatt aactcccctt
      361 cgcgatgctt cttcaccaga tacttccatt tccaaccgct ttcaagattt tcaagttgtt
      421 gatatttcat agcgatctca atgttaccgt gtaactcttt acagaatatc agctttttag
      481 gccagtgaag aaaagaatct ccatcctgtg agctttaacg cccatgcctg gagtattgtc
      541 ggtgctttct acgtgtgctg tagtgccggt tacggtataa tcgcggcttt gacaacagac
      601 taaaaaacat caactttgac cattacgaaa cttgcatggc gtgacctggt tcctgatacc
      661 gatagctatc aggaaatatt tgctcagcca catttgattg acgaaaacga tcctttattc
      721 agtgatactc aaccgcggct gcaatttgcg ctggagcagt tgctgcatac gcgagcatcc
      781 tcctctttta tgctggcgaa ggccccggaa gagtctgagt atctgaatct tattgccaat
      841 gccgcgcgta cgctacaaag cgatgcaggc caactggtgg gcggtcacta tgaggtttcc
      901 ggccactcca tccgcttacg tcacgcagtg agtgcagatg ataattttgc gactttaacg
      961 caagttgtcg ctgccgactg ggtagaagcg gagcaactct ttggctgcct gcgccagttt
     1021 aatggcgaca ttaccctgca gcctggtctg gtgcatcagg caaatggcgg tattctcatc
     1081 atctctttgc gtacactgct ggcgcaacct ctgctgtgga tgcggctgaa aaatatcgtt
     1141 aaccgcgagc gttttgactg ggttgcgttt gatgagtcgc gccctctccc cgtctctgtg
     1201 ccttcgatgc cattgaagct gaaagtcatt ctggtaggcg aacgcgaatc attggctgat
     1261 ttccaggaga tggagccaga gctttcagag caggctattt atagcgaatt tgaagatact
     1321 ctgcagattg tcgatgcgga gtcagtaacc cagtggtgtc gctgggtgac atttaccgcc
     1381 agacataatc acttacctgc accgggagcg gatgcctggc cggtacttat ccgcgaagca
     1441 gcacgctaca ccggtgaaca agaaacactt ccgcttagcc cgcagtggat cctccgccag
     1501 tgtaaagagg tcgcctccct gtgtgatggc gacaccttct ccggcgagca gctaaactta
     1561 atgctgcagc agcgtgaatg gcgcgaaggt ttcctcgctg aacgtatgca ggatgagatc
     1621 cttcaggagc aaatcctgat tgaaaccgaa ggcgaacgca tcgggcaaat taacgccctt
     1681 tcggtcattg aatttccggg tcatccacgc gcttttggcg aaccttctcg cattagctgc
     1741 gttgtgcata ttggcgatgg tgaattcacc gacatcgaac gcaaagcgga gcttggcggc
     1801 aatatccatg cgaaagggat gatgatcatg caagcgttcc tgatgtcgga actacagctt
     1861 gagcaacaga tccccttctc agcatcgctg acatttgagc agtcatacag tgaagttgat
     1921 ggagatagtg cctcgatggc tgaactctgc gccctgataa gcgccctcgc cgatgtgccg
     1981 gtgaatcaga gtatcgctat cacaggttca gtcgatcagt tcggtcgcgc ccagccggtc
     2041 ggtggtttaa atgagaaaat cgaaggcttc tttgctattt gccagcaacg tgagttaacc
     2101 gggaaacaag gtgtcattat ccccacagct aacgttcgcc atttaagtct tcacagtgaa
     2161 ctagtgaaag cggtagaaga aggcaaattc accatctggg cagtagacga tgtgactgac
     2221 gcactgccgt tattattaaa tctggtgtgg gatggcgaag gccaaacgac gctgatgcaa
     2281 accatccagg aacgtatcgc gcaagcatcg caacaggaag gacgtcaccg ttttccatgg
     2341 ccattacgtt ggctgaactg gtttattccg aactgatcgg acttgttcag cgtacacgtg
     2401 ttagctatcc tgcgtgcttc aataaaataa ggcttacaga gaacatggta gataaacgcg
     2461 aatcctatac aaaagaagac cttcttgcct ctggtcgcgg tgaactgttt ggcgctaaag
     2521 gcccgcaatt gccagcaccg aatatgctga tgatggaccg tgtggtcaaa atgaccgaaa
     2581 cgggtggtaa cttcgacaaa gggtatgttg aagcagaact ggatatcaat ccggatctgt
     2641 ggttcttcgg atgccacttt attggcgatc cggttatgcc gggatgcctg ggcctggacg
     2701 caatgtggca gctggtaggg ttctacctcg gctggctggg cggcgaaggt aaaggccgcg
     2761 cactgggcgt tggcgaagtg aaattcactg gtcaggtact gccgacagcg aaaaaagtga
     2821 cctaccgtat tcactttaaa cgcattgtta accgtcgtct gattatgggc ctggcggatg
     2881 gagaagtgct ggttgatggt cgtttgatct ataccgccag cgacctgaaa gtcggtctgt
     2941 tccaggatac ttctgccttc tgattataat cagacagagt atcaaaaggc gaaacctccg
     3001 caatgcggag gtttcttttt aaagagacag aatcaggcca ttactaccct gtccgccatg
     3061 gcttctcgcc agcctcccag ccattgtgac ctttgattca gcgtctgata gggacacatt
     3121 tcttttgagc gtccggcgat gccggcctga taaccacgtt gatgtgcccg ttccaggcga
     3181 tctcgttttt gtctcttcat gcctcgtttc cctcatactg gtttctggtg gaaaagaaaa
     3241 cagtgattac taaatgtgca atcacagtta agcaataccg cgaatcataa ctgccgtcaa
     3301 tgcgcaaaat tcacgccact gtcatatttg tgagctacac gggtatgtcg gttgtacaaa
     3361 aaatgtaaag aggaacaact ttttccgcac gttagaaaca aaaaaaccgc ctcaggcaac
     3421 ataccggaga cggttatatt tacaaatctt tataattaag gtagacgctt tatctcttgc
     3481 gcaatagaag cggcttcctg actccagaca ctggccagta ctttaaccat ctcatcgtaa
     3541 ccatcctgag tttgcactcc ttccagacgg aacggacgtt tgatcagttg tccctggtgg
     3601 ttcaacagcc actcaccact gacaatgacc ttgccatcat agcgaccgtt aaactccgtt
     3661 acggtaacat tgagcgtgtc ctgggcgctt cccagaggct gggaggcaac cacccagccg
     3721 ggcagttgcg tactcaggtt ggcaaccagg gtgttgcgca attgttgatc caacgggctg
     3781 gcccacaagt tgttgttggc aatcacatac ttcacatcac tggtttgata aaccacacca
     3841 ttccccgcca gatagtcagg aacagtgacc tgctctaccc ataacagacg attgccctgg
     3901 ctggcggtac tttgtgtacc gctctgcacc acaggtaact ggtaatagtt tttattaatt
     3961 tcgccggagc tgcatccggc cagccacagt gctgcaatcg tcactagcca ctttttcatt
     4021 gtttcgccct cttcggctct ggatcttttt tgtccttcgc ttcaaatacc agcgcgttac
     4081 tcttctcatt gagcgttttc agcaccggtt gcagttctcg caacacctga tcaaggcgct
     4141 gcatatccgc caccatcttg ttgtaggctg cggagccagg ctggaagccc tgcatgctgc
     4201 gattcaattc acgcaacgtt gactgcatat ccgtcggcaa ctgctgcatc gactggctgg
     4261 cgaggatctt gttcatgcta tccagcgtcg tttgcaggtt tttcattgtg cgctgacttt
     4321 cagaaagcgt actggttgcc tgttcaatca tcggattcaa tggcagtttg ttgatcttat
     4381 ccaacgcttc catcagtcgt tgctggattt gcgccaggcc gccgctaaca gtcgggataa
     4441 tctgataacc attaaattca cgaataccgg ttattgcagg cgtatttgga tagaaatcaa
     4501 gatcaacata cagtgcacca gtgaccaggt ttccggtttt cagcgatccg cgtaaaccac
     4561 gtttcaacaa ttcgccaagg tgctcaacaa catccgcatt ttcgccaagc tgcattttca
     4621 gccgctctgg ctcgatacga atcagtaccg gaatacggta atcatcgtta aatgtctgac
     4681 gcatattcgg cgcaaagaat ggcactttgc ttacggtacc caggcgaata ccccggaatt
     4741 ctaccggagc tcccggttgc agaccgcgta ccgaatcttt aaagaacatc agataatcaa
     4801 tgtgatcggt gtacaacgaa tcctgaatgc tcttctgatc atcatacaaa acgaaagctg
     4861 ttttcggtgc cactggctgc cctaaatcca gaccttccgg cacatcaaag ctaacaccgc
     4921 cactaagcag cgttgtcaat gagcccatct ccacacgcat ccccgctgac gtcagatcaa
     4981 ccgcaatgcc actatctttc cagaagcgaa cattgctggt caccagtcgg tcataaggtg
     5041 cattgatgaa cagttgataa ctgatattgc gtttttgcgt atcgaaggtg ctggtttcaa
     5101 ccgaacctac ccgatagcca cggaacagca ccggatctcc tggcgagagc tgcccggctt
     5161 ttttgctatc gagaatcaca cggatacctt tcgcatcagg cggggccaac ggtggcgagt
     5221 ccagcaaatc gtatttatcc attttgctgc ctttcgcgcc tggctgcagt tcgatataaa
     5281 ctccagacag cagcgttccc aggccgctaa tcccttcgcg accaatctgc ggtttcacca
     5341 cccaaaagac ggtgtcttta tgcagcaatt tttccatacc agaattcagc cgcactttga
     5401 tttcaacgtg cgtcaaatca tcagccagtg tggcgctttc aaccacgccg acgtcaacgc
     5461 tacggctttt aatggtggtt ttgccacctt caattccttc cgcattcgcg gtgatcaggg
     5521 tcacttccag tccctgatgg ctgtaatgat aaaaaagaac ccaggcccca atgagcgccg
     5581 tgacgatagg aaatatccac acgggagacc agttcttcac tttctggatt ttggcttccc
     5641 cattattaga ttccatactg tcaggactcc tcatgctctg attcaggttg acgatcccac
     5701 gacaaacgcg ggtcaaacgt catagcagaa aacattgtca ttatgacgac taaagcaaac
     5761 attaatgcac ccattgccgg ataaatactc attaaacctc ccatacgcac cagcgccgag
     5821 agcaccgcga taacgaaaac gtcaatcatc gaccagcggc ctacaaactc aacaacttca
     5881 taaatcaaat gcattctttc actgtcgcgc ttgccatgcc ctttggcatc ccaacacagc
     5941 cacgcgatgg cgatcatctt taacgttggc accataatac tggccagaaa gatcaccgca
     6001 gcgacgggat aagagccttc gctccataac agaatgaccc cagcgagaat cgtcgacggc
     6061 atcttcgagc ctaataaatc cgtcaccatg atgggcaaaa tattagccgg aagatacagc
     6121 atgatggacg ttacaagcag cgcgagtgtc cactgctggc tgtttctacg ccgaacgtac
     6181 cctttggtac tacaacgcgg gcacacgggt tcatcagcag gaaggattgc cgtacaacag
     6241 gagcacgaac gcagcccctg acgtatcccc gtgacgcctg gttttagcgg ctggcgcagt
     6301 tctggcatcg gggcgatgtc gtcccataac cagcgacgat caacgcactg aaaagcgcgc
     6361 agttgcagga cacaaaataa acaccagggg agaaagctgc tgcctacccc aatgctgccg
     6421 taagccatca gtttaacgaa actgaccagc acaccagcga ggaaaatctc cgccattccc
     6481 caggttttga gttgaaaaag cacccgtgcc agttgctctt ttaaacggac cggtaattcc
     6541 gcgcgattca ccagtaacag aatggttatc agacaaaacg cgggaaccag ttgcacaaac
     6601 aacaggaaaa aggtgccgag gctggcgtag tcctcagaaa aaagcacgcc gggaatttcc
     6661 agtaatgtaa tttcactggt aactcctgca acgttcatat tcacaaaagg aaacaagttg
     6721 gacagcaaca gcatgaacag tgcagccaac gcataggcgg tcggacgctg ccgaggggca
     6781 tcccacgcca cggttaacgt tgtgccacac cggggacatg tcgctttctg accatgctca
     6841 aggcgcggta acgccaccag catgtcacac tgcgagcaca ggatgtgctt cgcggcatga
     6901 tgatgttcgc acattggtac tccttaatga tgcgctattt tacagatggc taacaaagtg
     6961 ttggttttca tgcctgataa acccagttca tcaggcaatt ttaccgttgt cagctgtctc
     7021 tgtttaaatc gactattttg cgatcagcca ccatttttta acgcttcaag atactcccag
     7081 cgttcaaagg cttgctccag ctcctgctct gcagcagcca tatcggcgag cactttttgt
     7141 gtctgctcat gtggctgact gaagaaggaa gcatccgcca cctgcgtctg tagggcttcc
     7201 agcttcgcct ccagatcttc gagcaattgc ggtagctgct ccagttcgcg ctgcaattta
     7261 tagcttagtt tgctactgct gcgttttaca gtttctgctt ttgccgcggc ggcttcttcg
     7321 gtttttttca ccgcaggctg tttgagcgcc acatactgct cttgctgacc acgggcatca
     7381 tgataaccgc cgacataacg accaatttta ccgccgcctt cgaagatcca acattctgta
     7441 acggtgttat cgacaaactg acgatcgtgg ctaaccagca ataccgtgcc ctgatagctg
     7501 tcgatcagtt cttccagcag ttccagcgtt tcgacatcaa gatcgttggt cggttcgtca
     7561 agaatcaata agttgcttgg tttgaggaac aaacgcgcca gcagcaagcg gttccgctca
     7621 ccgccagaaa gcgcacgtac cggcgtcatc gcccgtttcg gatggaacag aaagtcctgc
     7681 aaatagccca atacgtggcg tggcttgccg ttaaccatca cctcttgctt accttcggca
     7741 aggttatcca tcaccgtttt atcgggatcc agttccgcgc ggtgctgatc gaaataagcc
     7801 acttccagtt tggtgccaac gtgaatacgc ccgctgtccg cttgaagctg atcgagcatc
     7861 agtttcagca gcgtggtttt gccgcaccca ttcggaccaa tcagggcaat tttgtcgcca
     7921 cgtagaacct gggccgaaaa atctttcacc agttgcttac cgtcaacctg gtagcaaacg
     7981 tcttccattt cgaaaacgat cttaccggag cggctggcct cttccacctg catctttgcg
     8041 gtacccatca cttcgcgacg ttcaccacgc tcgcgacgca ttgctttcag ggcgcgtacg
     8101 cggccttcat tacgggtacg gcgtgctttg atcccctggc ggatccacac ctcttcctgc
     8161 gccagtttgc gatcgaactc ggcattttgt agttcttcca cgcgcagggc ttcttctttt
     8221 tcaagcaggt actggtcgta attccctgga taggtcacca gcttgccgcg atcgagatca
     8281 acaatgcgcg tcgccatatt gcggataaac gaacggtcgt gggagatgaa aataatcgtc
     8341 ccattgaagg ttttaaggaa cccttccagc cagtcgatgg tttcaatatc caggtggttt
     8401 gtcggttcat caagcaacag cacgcgcgga ttactcacca gcgcgcgtcc taatgccgct
     8461 ttacgcaacc agccgccgga aagcgacgac agcgcaacat taggatctaa ccccagttgc
     8521 gccagcactt cgttgatgcg gttttccagc tgccacaggt tgtggtgatc caactgttcc
     8581 tgaaccttcg ccagttcgtt gagatttttc tcgctcgggt cgttcatcac caggcgcgaa
     8641 atatcgtgat agcgtttcag atattccgct tgttcttcaa tgccttcggc aacgaaatca
     8701 taaacgctac cctcaacgtt acgcggcggc tcctgttgca gacgagctac aatcaaatct
     8761 tgctcgtaaa taatgcgacc gtcatccagc ccttgttcac ggttgaggat tttcattaac
     8821 gtcgatttgc ccgcgccgtt gcgacccacc agacaaacac gttcgttatc ttcgatatgc
     8881 agttctgcgt tatcgagaag cggcgcgtcg ctgaacgaca gccatgcgcc atgcatactg
     8941 attaatgaca ttactattcc tttcaggctg cggtaatcag ccagcagttg tggatctggc
     9001 ggttacgggc gaaatcctgg gagagcgttt tttgcgtaat ttcttgtgct ttcagtccca
     9061 gttttgccag gccgtcgaga tccatacgga agccacgttt gttgttcgag aacatgatcg
     9121 tcccacctgc acgcagcaga cgtttcaaat ctttcatcag cgccatatga tcgcgctgaa
     9181 catcaaacgc atcttccatt cgttttgagt tagagaaggt tggcggatcg ataaagatca
     9241 gatcaaactg ttcatttgcc tcacgcagcc acgccaggca atcggcctga atcaggcgat
     9301 gcgcacgccc ggtcaggcca ttcagacgca ggttgcgttc tgcccactcc agataagtac
     9361 gcgacatatc cacggtggtg gtgctgcgcg cgccgcctaa tcccgcatgc acggtggcgc
     9421 taccggtata agagaacagg ttgaggaaat ctttgccttt gctcatctga ccgagcatac
     9481 ggcgggcgat acggtgatcg aggaacagac cagtatcgag ataatccgtc aggttcaccc
     9541 acaagtgagc gttatattcg gtaacttcaa gaaactcgcc cttctcgccc agtttctggt
     9601 actggttttt gcccttctgg cgttcacggg ttttcagcac cagtttgttt ggcgcaatcc
     9661 ccagtaccga aatggttgca gcgataatat cgaacagacg ctgacgcgct ttgtgagcat
     9721 caatagtttt tggcggcgca tactcctgca ccaccaccca gtcggcataa cggtcaacgg
     9781 caacgttata ttccggtaga tcggcgtcat acaggcggta acattcaatt ccttcctggc
     9841 gagcccactt ctcgaatttt ttgaggttct tacgcaggcg gttggcgtag tcttccgcta
     9901 ccatcgccgg tttgctgtct ggagtgcttt cggcaacgtg gtaatttttc tgtacgcagt
     9961 ccagcgggcc gtttttcgcc ttgtactgtt tgtctgcacg cagctgcaag cagcttagca
    10021 gatccggcga ggcactaaac aaagagagat tccagccacc aaactggttt ttcatgatcc
    10081 gacccagcag gctatgcagc gcaatcagcg ccggttcgct gtccagacgt tccccgtatg
    10141 gcgggttgct caacactgtg ccgtacggcc ctttcggcag cggattggtc agttgcgcga
    10201 catctttcac ctcaaaggtg atcagttcac caatccccgc aagacgggcg ttagtgcgcg
    10261 cacgttgaat cacccgtgcg tcgctgtccg aaccgtaaaa atgagagcta tactcagcca
    10321 ggcctttacg ggcacgagtt tgcgcttccg ctttcacttc ctgccagata gcttcatcat
    10381 gctgcaccca gccgctaaag ccccaacgcc cacggtgcaa gcctggtgcg cgatcggtcg
    10441 ctagcatcgc cgcttcaatc agcaacgtac cggaaccaca catcggatcg agcagcggtg
    10501 ttcctggctg ccagccggtt cgcatcacaa tagcggctgc cagggtttct ttgatcggcg
    10561 caataccagc acgatcgcga tagccacgca gatgtaaacc atcaccactc agatcaagag
    10621 cgatactggc ggtttcttta tgcagccaga cgttaacgcg gatatccggc gcatcgcgat
    10681 caacatttgg acgcggcaga tttttccgcg tgaaagcatc gacgatcgcg tctttcactt
    10741 tcatcgcgcc gtactgactg ttgcggatgg tgtcattcaa accactgaag tggacagcga
    10801 aggtcgcgcc aggattaaac atctctgtcc agttgatcgc ctgaacaccg agatagaggt
    10861 ctaaatcgct gtaaacctta cactcgccca gcggcaacat aatacgcgag gccaggcggc
    10921 tccacatcag gctctggtaa acaagccgtg tgtcgccctt gaaatggacc ccaccctgaa
    10981 ccacctggca ttcaacggcc cccaggtttt ccagttcagt ttttaacagc tcttccagcc
    11041 cacgggccgt actggcaaac agagaattca tatcgtcact tttactctaa gagcctaccg
    11101 cagtaggcgt aattgttgca gtcagtctgg acatggacag cgcggagaaa ccggagcgta
    11161 cacaagtacg tgaggatttc gagcacaacc cagggccaaa ctgacaaata aaatagccta
    11221 atgggacagg cgctaagaaa attgttgcgc attatagcta ataagcgcgt catgtcataa
    11281 agttgagggc ttattttcat ttgaggaccg caccgtggcg acattaaccc ggctttttat
    11341 tcatcctgtt aaatcgatgc gcggcattgg tcttacacat gctctggctg atgtcagtgg
    11401 tctggccttc gatcgcatct ttatgatcac ggaacctgac ggtacgttta ttaccgctcg
    11461 ccagtttccc cagatggtac ggtttactcc ttcacctgtg catgatggct tgcatctcac
    11521 cgcaccagat ggcagtagcg catatgttcg ttttgctgat ttcgccacac aagacgcacc
    11581 aaccgaagtt tggggcacac attttaccgc gcgaattgcg ccagacgcga tcaacaaatg
    11641 gctaagtgga tttttctccc gcgaagtgca attacgctgg gtggggccac aaatgacccg
    11701 gcgcgtgaaa cgccacaaca ctgtacctct gtcatttgct gatggctatc cttaccttct
    11761 tgctaacgaa gcctcgttac gtgatctcca acaacgttgt ccggccagtg taaaaatgga
    11821 gcaattccgc cccaatctgg tggtttccgg cgcgtcagcc tgggaagaag atcgctggaa
    11881 agtgattcgc attggtgatg tggtgtttga tgtggttaaa ccttgtagcc gctgtatttt
    11941 caccaccgtc agcccagaaa aagggcaaaa acatccggca ggcgaaccat taaaaacatt
    12001 gcaatctttc cgcactgccc aggataacgg cgatgtcgat tttggtcaga atttaattgc
    12061 ccgtaatagc ggcgtgattc gcgttggcga tgaggtggaa attctggcaa cggctccggc
    12121 aaaaatttac ggcgcagctg ccgctgatga taccgccaac atcacgcaac aaccggacgc
    12181 aaatgtagat attgactggc agggacaggc atttcgtgga aataaccaac aggtgttgct
    12241 ggagcaatta gaaaatcagg gaattcgtat cccttattct tgccgcgcgg gcatttgtgg
    12301 aagttgccgt gttcagcttt tagaaggcga agtcatgccg ctgaaaaaat cagcaatggg
    12361 cgatgatggc accattcttt gctgtagctg tgtaccgaag actgcactta agttggcgcg
    12421 ttagactgcc tgttcgaggc tgaagctgtc aacattaacc tgcggtttca gcctgtcgtt
    12481 catgatttta atggcgtcgc ccaactgcat cgcgcgaccc gcaataacaa cgcagggttg
    12541 tgccagcagg catagtgcgg cattttcgcc agattcaacg accagcaaat tgacctgctc
    12601 gtgagtatcg ctcaaccaaa cgcaggccgc atcgcccggc atgggcaccc acatttcacc
    12661 atgggaaaca aaatgccagc ttttcggcat ttgcggtttg agatagcgaa ttgctaccag
    12721 tgcattcagc accagttcgg ctttttgttc tttagataaa ttaaaatcgc ggcacttttc
    12781 ttcaaaagag aaatagagcg cggcgtcgtc aacgcaaaag cctgcgggag aaaaggcatc
    12841 tggagtcaac atcttgcgcg caaaacgtga gcgaaatagc ataccattgg ctaaatcgag
    12901 catcattcga tcgtgctctt catcgtaata ccaacgccag ttatcgtctg gtttaattcg
    12961 catctgcctc tcccctaccc tataaaaccg cagcaacaca atccattgtc gcttcgctta
    13021 ataacctcaa caataaaagt acaaaatgtc taaataagca acagcgatga atataaaaca
    13081 accagggctg aaaataaagc cctgattatc ttataaagag gaattcgaag aaataattag
    13141 atatgggtaa cgatttcttt aatcagcggc ggacctttaa aaataaaacc agaataaatt
    13201 tgcaccagtg aggcacccgc agcaatcttt tcacgcgcag cgataaccga gtcgatgccg
    13261 ccaacaccga tgatcggtaa gcgaccgttt aattccagtg acaagcggcg aataatttcg
    13321 gtgcttttta actgaagcgg acgaccactt aagccaccgg tttgatcgca atttttcatt
    13381 ccctgaacaa gagaacgatc gagtgtggta ttggttgcaa taacgccatc aatattatgg
    13441 cgaactaaac tatcggcaac ctggatcaat tcttcttcag aaagatccgg cgcgatcttc
    13501 actgcgatcg gcacatattt atggtgcatc gcttgcaaat cattttgctt atttttaatc
    13561 gcggttaaga gatcatccag cgcttcacca tattgcagcg tgcgtaatcc tggggtattc
    13621 ggcgatgaaa tattgatggc gatatatccc gcataggcat agattttttc catacaaatc
    13681 agatagtcat ctttgccctg ctccactggc gtatctttat ttttgccgat gttaataccc
    13741 aggacgccgt cataatgggc cttttttacg ttctctacga ggttatcaac gccaagatta
    13801 ttaaagccca tacggttgat caaaccttcg gcatctacca gacgaaagag acgcggcttg
    13861 tcattacctg gctgtggacg tggcgtgacg gtaccgatct cgatcgatcc aaatcccatc
    13921 gcgcctaacg cgtcaatgca ctccccgtct ttatcaagac cggctgccag accaagcgga
    13981 tttttaaacg tcaggcccat gcagttaaca ggtttcgcag gcactttctg ccgcaccagt
    14041 gcttcaaacg gcgttcctgt aatacggcgt aattgctgaa aagtaaactc atgagcgcgc
    14101 tctggatcga gctggaaaag ggctttacga acgaaggggt agtacatgaa ctctcctgga
    14161 ttcccggtgt gcaaaccggg ggcgtattat gtgcgagttc ggaccaaaag ggaattgacc
    14221 tgcggcaaaa aaaacgcaaa cgttttccga acctgtttca gggcagattt tgctccttct
    14281 ttgcactcaa cccgccgtaa tcatttatcg cccgccaggc gacatgacac ggtgcgattg
    14341 cctgacacca gcttgtctga cgctggcttt taggcggcac aactcccgca ttacttaacg
    14401 ctttaccatt tacgctcacc tgaatcaggt taatgtagta aggcgttggg ttagtgagtt
    14461 gtacaccctg cgaattccgt gtccagcgta attgttgata agcttccagc gggtcgcccg
    14521 gcaaaccttc gggtcgccag aacagtttaa ataccgagcg catcgccact tttacgctct
    14581 gattttcaac tttgccggat ggcacgctgg caatgcttaa ctcaaatagc gtttcgcgat
    14641 ccacaggcaa gatgtcgctt tccgttctca gcaagcgcaa cgtgcctgta gtaccgggct
    14701 tcaggagaat aagtggcgga gcggccagta atggtgctgg cactgtcgac gcttcccccc
    14761 cggcccaacg cgttgggcgg ttgattttac tgttaataag ccaggattcc tgactggtat
    14821 tagtcagtaa aatagatatc gattctctgt ctgccggaaa gataaatcgc gttccgccga
    14881 ccaccactcc tgcccgcaat tcacccgcca ccagcagcga taaaacggcg aaaatcaaga
    14941 gcgccaatct gttccacgta ttagtcataa tgaagtacaa atgtcgcgct ggcattcact
    15001 ttacccgact gcacatcgct attgacaggt cgcaattgcg cataaaaagt taatggcacc
    15061 ttaccgctat gtaggctgta gtctttactc gcctggttaa gcggaatcaa tatcttcgca
    15121 tcatccagaa tcgcaattgc cagcccgctg gctgcatccg taccactttc aatggacagc
    15181 atttcgctct gcactccgtc gggggttcct gaaaaggtga cgtcaaccgt gctgacatct
    15241 gttccgcaat cctgcaaatt aatgatgaaa gatttctccg ggccataatt cccatgaaaa
    15301 aaacgtgtca gcgagacatc gcctaactgc acattgattt gcttgctatc ctgtgccagc
    15361 acgcatgtat tacggcgaaa cgatccgcta atgttgaccg tgccatcatg ggcaaatagc
    15421 tcacagcccc ccgttaatcc cagtaagaac agaatccaga ttatccgttt caacatcact
    15481 ccttcactgg taaaccagat caaaatagag aaccgccgtc gcattaccgc ccgttgctcc
    15541 cgcctttgtg gacttataac gaagttgata tttgagtgtg ttatccccgg cttttaaggg
    15601 cgtaagaggc tggacctgat tgagcgggat ttcttgctga ctttgcgcat caagaatttg
    15661 cacggcaatc cccgttgcca taccaccgct tccgcctgtg tcagttagtt tgagcagtgt
    15721 cggcgtaagg gtgtcagcct cgccgctaaa aaggacgtcc gcgccagtaa tactagcggc
    15781 acaaccggtg atatcgatat tcaaatcagc agcagtgctt acggtattcg cggcctgaaa
    15841 atcactgata ttaaaatcac caatatgaat attttgtaat gcacttttgg tggcgacatc
    15901 acagccctga tcgaccacat tcccataaat atgcatacta acttgttcag gcccagccat
    15961 cccgacatgg atgttaagaa tgctaaagaa caagataacg cactgaaata tcgtttcttt
    16021 cttcatacgt tcacgcgtta atcaaaattt attctcaccg ttgccgtggc ttcaaatcgc
    16081 ccggtttctg gtggtacacc tgtcgtgctg actggataga cattcattcg cgcgatactg
    16141 tctgcatcat caggaataga gagcggtaat gttccaccag caggggaaat aatgttgttc
    16201 tggctatcgg tcaccaccac gcccacgcca ggacgtgacg tcttaatcgc ctggggatag
    16261 ctggggtctg tggttgccgt cagactcagg ttgacgttaa ctaaccccgc gtttcccgtg
    16321 ttacacctga ctggaattgc aagttctgcc gttctggccc caagaggctt atgccctaca
    16381 acacgaaagt tagccgcaaa tgcgtcgccc agatcgacct cgattgtttc tccggcattg
    16441 acggaacatg agccaagtga attgatcgta ccagacaggt aatacactaa aaccggtgta
    16501 cctgtagtcg tgcaggaatc acctgcgggt atgttgtagc attcaaataa tcgcgccagc
    16561 gccataggag gaatgacgac cgaaccaacg aacggctgca atattttcag ggaaaagcta
    16621 ccgctattac cgctagcccc ctggccacct aaacgttgac cgccgggatc gcaaatatgc
    16681 catcgtgtat ctgcccctgt gcccacgttt tcgaaaggaa ccgcagtagg agaactgcct
    16741 ttaacctgca ataaaacttt ggcggcaaga tagtcattaa ttttgtacca gttgtcgcct
    16801 aaatagacta acggtgaatc cgcggcgaat gcccatcggc cacttctgac attgtcttta
    16861 tcgcaatcac aggaaacata ataatcattc gattcgttcc actgataaaa tttgtcttta
    16921 actactccag ccgtattttc ttcttcctcc agggttttaa cgccaaaatt gacgccaata
    16981 tacggattcc cctgagtggt atgacactgc ccgctataag cagcagtttg agcgcaccat
    17041 agcattaaga cggcggcaaa aaatagtcgt agtaataaca cgcatttttc tccaaagatt
    17101 atctgcatat ggcatccatt cgcgtaatcg cagagttaat ttgttgttcg gtaagctggt
    17161 aactgatatg acaaatttga tcgggagctt cgccccagcg tacagttatc gctccctgag
    17221 cgggcaaacc ggacatatag agcgagccat catcatcaat aatgccgcta ttagtctgta
    17281 cgccgtccag cgtcgctatc gcgccaaaac gcagcggtat accctgcttt gatgttttca
    17341 ttaataccct tgctcccgca tgggcattga actccgccag aaccaacgcc cctttggtgg
    17401 gaaccacgtt gactactgca ttttcaagat ccacgttacg ttttaacgat gccgcatcaa
    17461 gtgcgattcg attttcccga taagaagtgg cataggggac aacggtatat ccgcgccagt
    17521 cagttttcag cccggtgctg ttcgccacac gagtattctc tgcgcctggt gctgcaatca
    17581 gcacgttagt ttcccccagc gattgcccca gggtaatgcc ctgtgaatgg gcaactaaac
    17641 tgcctgaaag agcgtagttc agttgttgtt gactgccgtt atcgctgtag ttgtagccca
    17701 ctcgggcatc tgcaaacgcc cctttgtagt ccatgctggc gctaccatta gccgttttcc
    17761 cctcgctgtt atatccctgt tgcacgctgt agctcaggtt accgtcatcg agtagtgtct
    17821 ccgttaaacc caggttttgt gtaatgtgcc cttcgttatc gatgctggtg ttagaggtca
    17881 tccaggcgta atttttttcc tggtatagct atcattactt ctggggagca acagattgag
    17941 cggcagggaa aaatttagtg caaacacctg atcagcatca ggttgaccac gggacttact
    18001 gtagttccag gaacccccca gcgagagatc tttaatttgc gtgttgtagc caaactgtaa
    18061 tagccgatcc tgttgatcgg tatgccagta agtttgctgg ctaccactta aataaaacga
    18121 accgtactcg cctaattgct gggagatatt gacctgcagt ttgccacgtt tggtgtaaaa
    18181 caaattgtaa taacgcgacc acatcggcgt atcttcatca tcaccgtcat taaattcgta
    18241 gccatccata tgtttataca tggtgtcgga aagggtgtag aaacccgacg tcgaatagcg
    18301 atagcccatc aattgaaaat tagtgcccaa ttggttgagc gttttggcat acagaaaacg
    18361 atatgactga cctttatgtt cgctttggtc tgccagcgta ctcttcgcct gggtcgcatc
    18421 gaacgaaatt gctccgaaat cgccaaggtt aaatcccaga ccaaacatgg cggcacggta
    18481 atattcagca tattgtccac caccatacca tgtcgttccc cacggtccgc cccactgcaa
    18541 cgtggcctag gcaaatttgc tctcctgctg ttcattacta ttggttctgt atttcgccag
    18601 tgtcaccgcg tatttgattc gcccctgacg ctggagtaat ggcacgctgg aaaacggtac
    18661 gctgtagcta ttgacgctgc cgtccgcttc tttgatttca actaacaaat caccgctcga
    18721 cgacgtggaa tagagatcac taatttcaaa agcaccaggc gatacataag tttggtaaat
    18781 ggtgtaacca ttttgcttaa tcgttatctg ggcattgctt ttggcgatac cacgcaccac
    18841 aggcgcaaac cctttcaggc tgtctggcag catgttgtca tctgacgcca gctgcacacc
    18901 acgaaagctg acactatcga aaaaatcgcc tgcagtataa tcatcaccta cggtcagttc
    18961 gccttttagt ggaataaccg cccgctgcaa atagctgctg agattcttcc attctgcggt
    19021 ttggccacta ctgcgactcc atgttgaatt gttgcgcaat ctccatgggc ctaaattaac
    19081 gccactgttt aaattcagaa aatagctgtc gccagaatca ctgtctgcgc tgctatgaat
    19141 actgttagcc ccgctaaacg aatatcccag taatagcgcg ttgatccctt catcccaacg
    19201 ttcagggggg acataaccac gggcattact tttgatggcg atttgcggta ctgaaatttc
    19261 cagacggagt ttattcacat taaacgttac gctggcatcc ggaataatct ccgccaatgg
    19321 cacacacgct tgtttgtcca gttgtttaaa agccggaaac gcatcagtat taacacccat
    19381 tgcatccagg ctttcagtcg tcaaacaggc ggcaatcccc ttctctggcg ttatctcaac
    19441 aaaattgacg ttgcgcgtat ctacaattgt ctgattcaga acaatatcga cacgataaac
    19501 ccctggcgtt attttttgtc ctttttcgaa acgagacaaa tcggccacca ttgagggatc
    19561 atctttgaaa aaagcaggat cgaaccaggt ttcagcttgt gcattgaacg ctgccgacac
    19621 aaacaccacc agcccaccaa taaacgatgg cactccacca gagcttaaca ggctgtgttg
    19681 tcgatgagtt ctatacatct cgctctctta actgaagacg gcttacgatt tacagttgag
    19741 ttgtggcgtt tccgccccgt aatcattgat ggttcgatac gttacgctgc tatttttggc
    19801 cccctttggc agagagaatt ggtatttatc aaagggggaa atcatttgat ttttaactac
    19861 ctttccccct attttcaggt ctgtcagggt aatgaaataa ggtgttgggt tactgaccgt
    19921 aaccatgtca gctgacgaag tgcaacgcag tttcgccgga gcctcgccta agggatacgc
    19981 taaacctttt ggtcgccaga aaagtttgat gacgctttgt gtggcaatct tcagggtatt
    20041 tccctcttcc cgcttcgttg ttgaagggac tgcccgtacg ctagtgaaga aaagggtttc
    20101 tctgtctttc gccaacggcg caccaatgta cataatgcgt aacagattct cattagccgg
    20161 attcagcaca aatagcggtg gtgtaataat aaaatctgct gattttttat tatctggggt
    20221 catcacccat gactgaataa gataaggaac atccggactg gtgttacgta cacccagtga
    20281 aatttgctct tcaccttctg aataaacaag acgtgttgct gcaagtccaa tcccgccttt
    20341 acctgccgcc catgcggatg agcagctgag taatattgcc gttaaactta cggtcacaat
    20401 tccctttgtt atgcaagttt tcataaatag tcactcttaa aaaatatgaa tatcccctcc
    20461 agaacactag aggggtacta attaataaaa caattaatga gttctattaa taatagttaa
    20521 tatggaaatt tgttaccgca ttcacattac cagaagtcgc tttccccgta gcgatataat
    20581 caactttaaa ggtcactgtt tgctcagcag aatccgctaa ctgaatattc gacgtttcgg
    20641 taccgggagt taccgatgta gaaccatctg gcagatacat ttgtagagca acattcgtcg
    20701 ctgcatcggt ggtagcctgg ttggcaaatg ctgtaggttg cacgccgtca gccgtaccac
    20761 taaaggtaaa cgtcgcattt ttagttacag taacatcaca gccttctaag gcaattggta
    20821 catcagtttt aacgccagag actgctccat tagtttgcaa cttggtggcg ctgacatttg
    20881 gcaatgtgac tactgaatct ttcgtcgcag ctaccagggt acaagctgga gcaataactt
    20941 taccattaaa tgttactttg ccatccgtga tagcattatc atcagctgcc ataacgctgg
    21001 acgttgcaca taccacagtt ataaacgccg tcaatacact ttttttcatc gtcatcatcc
    21061 tgatctcttc gtaaatatga taatgc
//
LOCUS       DAJTZL010000066        21003 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.066, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000066 DAJTZL010000000
VERSION     DAJTZL010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21003
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.066"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..170)
                     /locus_tag="OO544_002070"
     CDS             complement(<1..170)
                     /locus_tag="OO544_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6767668.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYG"
     gene            342..1562
                     /gene="coaBC"
                     /locus_tag="OO544_002071"
     CDS             342..1562
                     /gene="coaBC"
                     /locus_tag="OO544_002071"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HCR6767669.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEVAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            1540..1998
                     /gene="dut"
                     /locus_tag="OO544_002072"
     CDS             1540..1998
                     /gene="dut"
                     /locus_tag="OO544_002072"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HCR6767670.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDSTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            2105..2701
                     /gene="slmA"
                     /locus_tag="OO544_002073"
     CDS             2105..2701
                     /gene="slmA"
                     /locus_tag="OO544_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HCR6767671.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(2738..3379)
                     /gene="pyrE"
                     /locus_tag="OO544_002074"
     CDS             complement(2738..3379)
                     /gene="pyrE"
                     /locus_tag="OO544_002074"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HCR6767672.1"
                     /translation="MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KNHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RSEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(3445..4161)
                     /gene="rph"
                     /locus_tag="OO544_002075"
     CDS             complement(3445..4161)
                     /gene="rph"
                     /locus_tag="OO544_002075"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HCR6767673.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAVCDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            4288..5151
                     /locus_tag="OO544_002076"
     CDS             4288..5151
                     /locus_tag="OO544_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HCR6767674.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            5373..6197
                     /gene="dinD"
                     /locus_tag="OO544_002077"
     CDS             5373..6197
                     /gene="dinD"
                     /locus_tag="OO544_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HCR6767675.1"
                     /translation="MNEHHQPFEEIRHYGTEGQEFWSARELAPLLDYRDWRNFQKVLA
                     RATQACEASNQAASDHFVETTKMVVLGSGAQRELEDVHLSRYACYLVVQNGDPAKPVI
                     AAGQTYFAIQTRRQELADDEAFRQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAI
                     DFAIFQNHGYRGLYGGLDQKAIHQRKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDGVNSKQQANTTHFDVGSKVRQTIQELGGTMPEELPTPQVSINQLENSVKITEKK"
     gene            6489..7106
                     /locus_tag="OO544_002078"
     CDS             6489..7106
                     /locus_tag="OO544_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HCR6767676.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(7103..8785)
                     /gene="ligB"
                     /locus_tag="OO544_002079"
     CDS             complement(7103..8785)
                     /gene="ligB"
                     /locus_tag="OO544_002079"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HCR6767677.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFGSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MVDKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQGNSDTLNSL
                     AVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEIARVRNEWWKAKLPFVT
                     DGVVVRAAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARKQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQRLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            9043..9666
                     /gene="gmk"
                     /locus_tag="OO544_002080"
     CDS             9043..9666
                     /gene="gmk"
                     /locus_tag="OO544_002080"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HCR6767678.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            9721..9996
                     /gene="rpoZ"
                     /locus_tag="OO544_002081"
     CDS             9721..9996
                     /gene="rpoZ"
                     /locus_tag="OO544_002081"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HCR6767679.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            10015..12123
                     /gene="spoT"
                     /locus_tag="OO544_002082"
     CDS             10015..12123
                     /gene="spoT"
                     /locus_tag="OO544_002082"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HCR6767680.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            12130..12819
                     /gene="trmH"
                     /locus_tag="OO544_002083"
     CDS             12130..12819
                     /gene="trmH"
                     /locus_tag="OO544_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HCR6767681.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            12825..14906
                     /gene="recG"
                     /locus_tag="OO544_002084"
     CDS             12825..14906
                     /gene="recG"
                     /locus_tag="OO544_002084"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HCR6767682.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDALKNKLLAALPFKPTGAQARV
                     VAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFEPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACMTEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(15075..16280)
                     /gene="gltS"
                     /locus_tag="OO544_002085"
     CDS             complement(15075..16280)
                     /gene="gltS"
                     /locus_tag="OO544_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HCR6767683.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            16560..17951
                     /gene="xanP"
                     /locus_tag="OO544_002086"
     CDS             16560..17951
                     /gene="xanP"
                     /locus_tag="OO544_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HCR6767684.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            18072..19781
                     /locus_tag="OO544_002087"
     CDS             18072..19781
                     /locus_tag="OO544_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HCR6767685.1"
                     /translation="MKFIGKLLLYILIALLVAIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMVHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANVPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSGEVTSAE
                     LVQ"
     gene            complement(19833..>21003)
                     /gene="yicI"
                     /locus_tag="OO544_002088"
     CDS             complement(19833..>21003)
                     /gene="yicI"
                     /locus_tag="OO544_002088"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HCR6767686.1"
                     /translation="LAIYDFTNPDACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQW
                     FDGSDPQKMHNHYAYIYNELVWNVLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYA
                     NYESMAESLRGGLSIGLSGFGFWSHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSK
                     SYRVPWAYDDESCDVVRFFTQLKCRMMPYLYREAARAKARGTPMMRAMMMEFPDDPAC
                     DYLDRQYMLGDNVMVAPVFTEAGEVQFYLPEGRWTHLWHNNELDGSRWHKQQHGFLSL
                     PVYVRDNTLLALGNNDQRPDYAWHEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAT
                     RTGNTITVTGAGEARNWTLCLRNIVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
BASE COUNT         5082 a         5713 c         5386 g         4822 t
ORIGIN      
        1 ccataaagag agccgaaatt ctccagcatc tcttttgcca gggttaatac atctttaccg
       61 cgcgttccgg tacgcagaaa tagcgccagc agctcgacat ccgttaaggc gctaatacca
      121 aacttcagca ttttttcgcg cggcatcaac agctgtgcat tgtttttcac cttcacctcc
      181 tttgtggtgc tcgcatcctg tcatagccgt ttcgttaaat cgacggccag ttttcaatcc
      241 tggaaagcgc ctcgcaaagt gaatctcagg tgaaggcacg acaacaaaag tattgtgata
      301 aaatcgccaa cttctggtgt caccctacag gaaaaatcat catgagcctg gccggtaaaa
      361 aaatcgttct cggcgttagc ggcggtattg ctgcctataa aacccctgaa ctggtgcgtc
      421 gtttgcgcga tcgcggggcc gacgtccgcg tagccatgac cgaagcggca aaagccttta
      481 tcaccccact tagcttgcag gcggtttctg gttatcccgt ttccgacagt ctgctggacc
      541 cggcagccga agccgctatg ggccatattg agctgggtaa atgggctgat ttagtgattc
      601 tcgcccctgc cacggcagat ttgattgccc gtgttgctgc cggaatggcg aatgacctgg
      661 tatcgacgat ttgtctggct acacctgcgc ctgtagccgt gctccccgcc atgaaccagc
      721 agatgtaccg tgccgctgcc acgcagcata atttagaggt gcttgcttcc cgtggtttgc
      781 tcatctgggg gccagacagt ggcagtcagg cttgtggtga tatcggtcct gggcgaatgc
      841 tcgatccgtt aaccattgtg gatatggcgg tagcgcattt ttcgcccgtc aacgacctga
      901 aacatctgaa cattatgatt accgccggcc cgacgcgtga accgctcgat ccggtgcgtt
      961 atatctctaa tcacagctcc ggcaagatgg gttttgctat cgccgccgcc gctgcccgtc
     1021 gtggcgcgaa cgtcacgctg gtatcaggcc cggtttcgct accgacgcca ccgtttgtta
     1081 aacgtgttga tgtgatgacc gcgctggaaa tggaagtcgc cgtgaatgct tctgtacagc
     1141 agcaaaatat ttttatcggc tgcgccgccg tggcggatta tcgcgcagct accgtggccc
     1201 cagagaaaat caaaaagcag gccacgcagg gtgatgaatt aacaataaaa atggttaaaa
     1261 accccgatat cgtcgcaggc gttgccgcac taaaagacca tcgaccctac gtcgttggat
     1321 ttgccgccga aacaaataat gtggaagaat acgcccggca aaaacgtatc cgtaaaaacc
     1381 ttgatctgat ctgcgcgaac gatgtttccc agccaactca aggatttaac agcgacaaca
     1441 acgcattaca ccttttctgg caggacggag ataaagtctt accgcttgag cgcaaagagc
     1501 tccttggcca attattactc gacgagatcg tgacccgtta tgatgaaaaa aatcgacgtt
     1561 aagattctgg acccgcgcgt tgggaaggaa tttccgctcc cgacttatgc cacctctggc
     1621 tctgccggac ttgacctgcg tgcctgtctc gacgacgccg tagaactggc tccgggtgac
     1681 tctacgctgg ttccgaccgg gctggcgatt catattgccg atccttcact ggcggcaatg
     1741 atgctgccgc gctccggatt gggacataag cacggtatcg tgcttggtaa cctggtagga
     1801 ttgatcgatt ctgactatca gggccagttg atgatttccg tgtggaaccg tggtcaggac
     1861 agcttcacca ttcaacctgg cgaacgcatc gcccagatga tttttgttcc ggtagtacag
     1921 gctgaattta atctggtgga agatttcgac gccaccgacc gcggtgaagg cggctttggt
     1981 cactctggtc gtcagtaaca catacgcatc cgaataacgt cataacatag ccgcaaacat
     2041 ttcgtttgcg gtcatagcgt gggtgccgcc tggcaagtgc ttattttcag gggtattttg
     2101 taacatggca gaaaaacaaa ctgcgaaaag gaaccgtcgc gaggaaatac ttcagtctct
     2161 ggcgctgatg ctggaatcca gcgatggaag ccaacgtatc acgacggcaa aactggccgc
     2221 ctctgtcggc gtttccgaag cggcactgta tcgccacttc cccagtaaga cccgcatgtt
     2281 cgatagtctg attgagttta tcgaagatag cctgattact cgcatcaacc tgattctgaa
     2341 agatgagaaa gacaccacag cgcgcctgcg tctgattgtg ttgctgcttc tcggttttgg
     2401 tgagcgtaat cctggcctga cccgcatcct cactggtcat gcgctaatgt ttgaacagga
     2461 tcgcctgcaa gggcgcatca accagctgtt cgagcgtatt gaagcgcagc tgcgccaggt
     2521 attgcgtgaa aagagaatgc gtgagggtga aggttacacc accgatgaaa ccctgctggc
     2581 aagccagatc ctggccttct gtgaaggtat gctgtcacgt tttgtccgca gcgaatttaa
     2641 ataccgcccg acggatgatt ttgacgcccg ctggccgcta attgccgcgc agttgcagta
     2701 atatgacgcc ggatgacttt tcatccggcg agtttcttta aacgccaaac tcttcgcgat
     2761 aggccttaac cgccgccaga tgttccgcca tttccggctt ctcttccagg taagcaatca
     2821 ggtctttcag ggtgatgata gagatcactt tgcagttgta atcacgctca acttcctgaa
     2881 tcgccgaaat ctcgctgcgc ccccgttcct gacgatcgag cgaaatcaac acgccagcaa
     2941 gcgtcgcgcc attggcctga ataatttcca tcgactcacg aatcgccgtt ccggcggtga
     3001 tcacatcatc taccagcatt acgcgtcctt gtaacgcgct accaaccaga ttgccgcctt
     3061 caccgtggtt ttttgcctct ttgcggttaa agcagtacgg caggtccagg tcatgatgct
     3121 ccgccagcgc cacggcggtt gtggtggcaa tcgggatccc tttgtaagca gggccaaaca
     3181 gcagatcgaa ctcaatgccg gaatccacca atgcttcagc gtaaaaacgg cctaacagtg
     3241 ccaggtcgcg cccggtatta aacagcccgg cgttgaagaa ataggggctt ttacgcccgg
     3301 atttcagcgt aaactcgcca aactttaaca cctgcttgct aagcgcaaat tcaataaact
     3361 ggcgctgata tggtttcatg ccttcgctcc tcatcttact tttctacaga caaaaaaaag
     3421 gcgactcatc agtcgcctta aaaatcagtt tgccagcgcc gccttctgcg tcgctacaat
     3481 ggattcgatt ccccctcggg ccagagccaa caaggtgagt aattcttcgt gggtgaacgg
     3541 ttcgccttct gccgtcccct gcacttcaat gatgcgcccg tcttcggtca tcactacgtt
     3601 catgtcggtc tctgcggcag agtcttcaac gtattccaga tcgcaaaccg cttcgccgtt
     3661 cacaatcccg acagaaaccg cggctaccat ccctttcatc ggattggttt tcagcttacc
     3721 gttttccacc agcttctgta gcgcatctgc cagcgccacg caggcacccg taatcgaagc
     3781 ggtacgagtg ccaccatcag cctgaagcac gtcgcagtcc agcgtaatgg tgaactcacc
     3841 cagcgctttc aaatctactg ccgcgcgaag cgcacgggcg atcagacgct ggatttccat
     3901 tgtgcgtcca ccctgcttac ctttcgccgc ttcacgagcg ttacgggtgt gggtagaacg
     3961 cggcagcata ccgtactctg cggtgatcca gccctggccc tgacctttca ggaagcgcgg
     4021 cacgccttct tcaatagagg cggtacacaa cactttggta tcgccaaatt cgaccagcac
     4081 cgagccttct gcatgttttg tatagttacg agtcagggta acgggacgca cctgattatt
     4141 gctacggcct gctggacgca tattgaaatc tccggcttga aacaaatgtg gctgcgcatt
     4201 atacggactt ccggaggtta ttcctatcct gacaaggcat cgatggctat aatccttcca
     4261 cctctccttt tataaacagg aacgtctatg atccgcagta tgaccgccta cgcccggcgt
     4321 gaaatcaagg gtgaatgggg gagcgcaacc tgggaaatgc gctcggtaaa ccagcgttat
     4381 ctggaaactt actttcgtct gccggagcag ttccgtagcc ttgaacctgt cgttcgcgag
     4441 cgtattcgtt ctcgcctgac gcgcggtaaa gtggaatgta ccctgcgcta tgagccagat
     4501 gttagcgcgc aaggtgagct gatcctcaac gaaaaactgg ctaaacagct ggtaactgcc
     4561 gcgaactggg taaaaatgca gagtgacgaa ggggaaatca acccggttga tattctgcgc
     4621 tggccgggcg taatggcagc ccaggagcag gatctcgacg cgattgccgc tgaaattctc
     4681 gcggcgttgg atggtaccct ggacgacttt atcgtcgcac gtgaaaccga aggtcaggcg
     4741 ctgaaagcgt tgatcgagca gcgtctggaa ggcgtcaccg ccgaagtggt caaagtccgc
     4801 gcccatatgc cggaaatcct gcaatggcag cgtgagcgtc tggtcgcgaa gctggaagat
     4861 gctcaagtgc aactggaaaa caaccgcctg gagcaggagt tggttctgct ggcacaacga
     4921 attgatgttg ccgaagaact ggaccgcctg gaagcgcatg tcaaagagac ctacaacatt
     4981 ctgaagaaaa aagaagccgt tggtcgtcgt ctggacttta tgatgcaaga gttcaaccgc
     5041 gagtcgaaca ctcttgcgtc gaaatctatc aatgccgaag tgacgaattc tgccatcgag
     5101 ctgaaagtgt tgattgagca gatgcgcgag cagattcaga atattgagta actcgtaaca
     5161 ctaattctta cctttcagcc ttcacaaatg ccgcccactc aaacagagcg gcatttttct
     5221 tccccgcaac attcaattct gttttgcgta cctgctccag attttgcgat gtttttttgc
     5281 gcagcgcagt gagaacgtga gatactcaca actgtatata aatacagtta tagacttact
     5341 ttctttgcaa tcgatatcac atggagtggg caatgaacga acatcatcaa ccttttgaag
     5401 aaatacggca ttatggcact gaagggcaag aattttggtc agcgagagaa ttggcaccgt
     5461 tactggatta tcgggactgg cgcaattttc aaaaggtact ggcccgcgcg actcaagcgt
     5521 gcgaagccag caatcaagct gcatctgacc atttcgttga aaccaccaaa atggttgtat
     5581 taggttcagg ggctcaacga gaacttgaag atgttcatct ctctcgctat gcctgttacc
     5641 tggtagtaca aaacggcgac cctgcgaaac cggttattgc ggcagggcaa acttattttg
     5701 ctatccagac ccgacggcag gagctggctg acgatgaagc tttcaggcaa cttcgtgaag
     5761 acgaaaagcg tctttttctg agaaatgagt tgaaagaaca taacaaacaa ttagttgagg
     5821 cagcgcagca agctggggta gcgacagcta tcgatttcgc catatttcag aatcatggtt
     5881 accgggggct gtatggtgga ttagatcaga aagccatcca tcagcggaag gggctgaaaa
     5941 agagtcagaa gatcctggat catatgggtt caacagaact ggcggctaat ctctttcgag
     6001 ctacccaaac agaagaaaaa ctcaagcggg atggcgtaaa ttcaaaacag caagctaata
     6061 ccacacactt tgacgtgggt agcaaggtga ggcaaaccat tcaggaactt ggcggaacca
     6121 tgcctgagga gttgccgacc ccgcaggtca gcatcaatca gttggaaaac agtgtaaaaa
     6181 ttacagagaa gaaatagcag aaactaaaaa ctctttttct taattgagat acccgatgcg
     6241 taagccaaag tccaggtgca gtgaacacaa tggctaaaca ttgcaccttt cttttccacc
     6301 cctcaatttt aacctatttt ttcttatgca ttttctcaga caagaagtca gaagaatgca
     6361 tctacactac agaaaatagc gatttcacat aactacaagt tatcaatttc ctctcacctt
     6421 aaaaaatctc aatcgtgaca atacgcacaa atcgctaccc tgccagacag atttttaggg
     6481 agagaaccat gctgttacac attttgtatt tggttggcat tactgccgaa gccatgacgg
     6541 gggcgctggc ggccggacga cggcgcatgg atacatttgg cgtaattatt attgcgaccg
     6601 ccaccgcaat tggcggaggg tcagtgcgcg atattctgct gggccactat ccgctcggtt
     6661 gggtcaaaca cccggaatat gtgattatcg tcgctaccgc cgcagtactc accactatcg
     6721 ttgctcctgt aatgccttac ctgcgcaaag tgtttctggt gctcgatgcg ctcggactgg
     6781 tggtcttttc tatcatcggc gcacaggttg cactggatat ggggcacggc ccaattattg
     6841 ccgttgtcgc ggcggtaacc accggcgtgt ttggcggcgt tttgcgcgat atgttctgta
     6901 aacgcatccc actggtattc cagaaagagt tgtatgccgg tgtctcattt gcctccgccg
     6961 tgctgtacat cgcgctgcaa cactatgttt ctaaccatga tgtggtgatt atctccaccc
     7021 tggtattcgg cttttttgcc cgcttactgg cgctacgtct taagctggga ttaccggtct
     7081 tttactacag ccacgaaggt cactaaggtt caaaacctgt gatctgctgg gcagccagcc
     7141 aactgcccag cttcttgatt tgcgcatttt ccttccattc aataacctgt ctggcgcgcc
     7201 cagatccagt ccccggcagc cgctgccaga actgctccgt gctaaataaa agttgcgacc
     7261 aggaccgttc atcactggca ttaagcgccg cccgggttaa cggtattccc attgccatca
     7321 cccagcgagt aaaaggctgc ttacgagcca gattaaactg atgccataac tgcgcacttt
     7381 tacttttcgc gatccccggc gtgttctgta attgctctgg cgttaataaa agccaggaaa
     7441 agatatgctc aaagcgatga gtctgatgca gcgcgcgcca gccggcctca ccaatgccat
     7501 ccagcccaag aacctgtttt gaccccagcc agactaagcg tgaaatgaac tgttcctgac
     7561 aaacatcaga agcaaagtag caggtcaacg agttaaagcg gttttctggt ggtgtcggtt
     7621 ttgtacgttc tgcaccgcgc cagaccacat catcaatgcg aggaataccc tgaccggcaa
     7681 ggctgacgag gatctgatca ccaggcgcaa tatcccactc ctgccagcgc ctgacggaac
     7741 caatattcac ccgctggact tttttatcat ccagcatgac aggtgcgagt gacgcaacca
     7801 ccgatatttt accgctctta cccaccgcaa actgaatcgc cttcacttcg gcaacctgag
     7861 ctacaggttg atatttccag gccaccaacc attctgcctg gcctggtagc caatggcggg
     7921 attcgggttc cttagccgct cgcacaacca cgccatcggt gacgaagggt aatttcgctt
     7981 tccaccactc attgcgtacg cgcgcaattt catcagcatt tttaaccgca cgggtatacc
     8041 tctgcgttag agtaaaacct gcggtagcca gctcctttaa acgatcggtc attaactgcg
     8101 gtccatccgg ccatgcccag acaaaaacag ccagtgagtt tagcgtgtcg ctattcccct
     8161 ggcgcatcat caggccagca acttttgcgc gggcatttat tccccccatt tgttgttgga
     8221 tatgcccctc gcgctggaga aatatttccc cctgaagcgt actgttggct aaaggcccgc
     8281 taacggtttg cggcacagcg gaaattaagc taactttctg cgtccagtcc tcgcctttca
     8341 ggccattacc gcgactgatt gctttgttca gtttcccgtc ccgataaacc agggttaccg
     8401 caacgccatc aacttttggc tgcacccaaa gatcgctacg ttctcgcatc cacagactta
     8461 atgcgttttt atccaccatt ttgcgtacac ccgtatgggc aaccggatgc ataaccgcac
     8521 catttaaagg cggcatcatc acgtcgcggg gctcgctccc aaagcagcgt tgccactgcg
     8581 taagacgggc gcttaactga tcgtaaacac cgtcttccac ctcacttttc ccttccttcc
     8641 agtagtcatc gtcccactgt tttatttgct gttgcaggcg ggaaatttct tcctgtgctc
     8701 tggctggcga ccaggccgga cagaccgccc acacagatga ttgccagcac aagatactta
     8761 ttaatatcgc catccatact ttcatcatca cctccgctgt agatagtcag gcagatatac
     8821 aacgtgatga aagcaaagcc gagtggcaaa aacggagtct gcgaggacgc ttcctgaaaa
     8881 tcgtctttat tgcaatgaat gacaggcaaa tgcggaagca gctacgcaaa acgcaacaac
     8941 tttgcgcaaa aagtgtgagc aagggctacg tcacatggcc gcgccgtgta taataagctc
     9001 gtatgtaggc tttatttcgc taatcacata cgaaagatac tcatggctca aggcacgctt
     9061 tatattgttt ctgcccccag tggtgcgggt aaatccagcc tgattcaggc tttattaaaa
     9121 acccaaccgt tgtatgacac ccaggtttct gtttcacaca ccacacgcca accgcgtcct
     9181 ggtgaagtcc acggtgaaca ttatttcttt gttaatcatg atgaatttaa agaaatgatt
     9241 agcagagatg cgttcctcga acacgcagaa gtttttggta attactatgg cacttcgcgt
     9301 gaggccattg agcaagtact ggcgaccggt gtcgatgttt ttctcgatat cgactggcag
     9361 ggcgcgcagc aaattcgcca gaagatgccg cacgcgcgga gtatctttat tttaccgccg
     9421 tccaaaattg aactggaccg ccgtctacgc ggtcgcggtc aggacagcga agaggtcatt
     9481 gcaaagcgta tggcgcaagc tgttgcagaa atgagccatt acgccgaata tgattatctg
     9541 attgtgaatg atgacttcga taccgcgttg accgatttga agaccattat tcgcgccgaa
     9601 cgtctgcgca tgagccgcca aaagcagcgt catgacgctt taatcagcaa attgttggca
     9661 gactgaacct gatttcagta tcatgcccag tcatttcttc acctgtggag ctttttaagt
     9721 atggcacgcg taactgttca ggacgctgta gagaaaattg gtaaccgttt tgacctggta
     9781 ctggtcgccg cgcgtcgcgc tcgtcagatg caggtaggcg gaaaggatcc gctggtaccg
     9841 gaagaaaacg ataaaaccac tgtaatcgcg ctgcgcgaaa tcgaagaagg tctgatcaac
     9901 aaccagatcc tcgacgttcg cgaacgccag gaacagcaag agcaggaagc cgctgaatta
     9961 caagccgtta ccgctattgc tgaaggtcgt cgttaatcac aaagcgggtc gcccttgtat
    10021 ctgtttgaaa gcctgaatca actgattcaa acctacctgc cggaagacca aatcaagcgt
    10081 ctgcggcagg cgtatctcgt tgcacgtgat gctcacgagg ggcaaacacg ttcaagcggt
    10141 gaaccctaca tcacgcaccc ggtagcggtt gcctgcattc tggccgagat gaaactcgac
    10201 tatgaaacgc tgatggcggc gctgctgcat gacgtgattg aagatacacc cgccacctac
    10261 caggatatgg aacagctttt tggtaaaagc gtcgccgagc tggtagaggg ggtatcgaaa
    10321 cttgataaac tcaagttccg cgataagaaa gaggcgcagg ccgaaaactt tcgcaagatg
    10381 attatggcga tggtgcagga tatccgcgtc atcctcatca aacttgccga ccgtacccac
    10441 aacatgcgca cgctgggctc acttcgcccg gacaaacgtc gccgcatcgc ccgtgaaact
    10501 ctcgaaattt acagcccgct ggcgcaccgt ttaggtatcc accacattaa aaccgaactc
    10561 gaagagctgg gttttgaggc gctgtatccc aaccgttatc gcgtaatcaa agaagtggtg
    10621 aaagccgcgc gcggcaaccg taaagagatg atccagaaga ttctttctga aatcgaaggg
    10681 cgtttgcagg aagcgggaat accgtgccgc gtcagtggtc gcgaaaagca tctttattcg
    10741 atttactgca aaatggtgct caaagagcag cgttttcact caatcatgga catctacgct
    10801 ttccgcgtga tcgtcaatga ttccgacacc tgttatcgcg tgctcgggca aatgcatagc
    10861 ctgtacaagc cgcgtccggg tcgcgtgaaa gactacatcg ccattccaaa agcgaacggc
    10921 tatcagtctt tgcacacctc gatgatcggc ccgcacggtg tgccggttga ggtccagatc
    10981 cgtactgaag atatggatca gatggcggag atgggtgttg ccgcgcactg ggcttataaa
    11041 gagcacggcg aaaccagcac caccgcgcaa atccgcgccc agcgctggat gcaaagcttg
    11101 ctggagctgc aacagagcgc cggtagttcg tttgaattta tcgagagcgt taaatccgat
    11161 ctcttcccgg atgagattta cgttttcaca ccggaagggc gaattgtcga gctgcctgcc
    11221 ggtgcaacgc ccgtcgactt cgcttatgca gtgcataccg atatcggtca tgcctgcgtg
    11281 ggcgcacgcg ttgaccgcca gccttacccg ctgtcgcagc cgcttaccag cggtcaaacc
    11341 gttgaaatca ttaccgcacc gggcgctcgc ccgaatgccg cgtggctgaa ctttgtcgtt
    11401 agctcaaaag cgcgcgccaa aattcgtcag ttgctgaaaa acctcaagcg tgatgattct
    11461 gtaagcctgg gccgtcgtct gctcaaccat gctttgggtg gtagccgtaa gctgaatgaa
    11521 atcccgcagg aaaatattca gcgcgagctg gatcgcatga agctggcaac gcttgacgat
    11581 ctgctggcag aaatcggact tggtaacgca atgagcgtgg tggtcgcgaa aaatctgcaa
    11641 catggggacg cctccattcc accggcaacc caaagccacg gacatctgcc cattaaaggt
    11701 gccgatggcg tgctgatcac ctttgcgaaa tgctgccgcc ctattcctgg cgacccgatt
    11761 atcgcccacg tcagccccgg taaaggtctg gtgatccacc atgaatcctg ccgtaatatc
    11821 cgtggctacc agaaagagcc agagaagttt atggctgtgg aatgggataa agagacggcg
    11881 caggagttca tcaccgaaat caaggtggag atgttcaatc atcagggcgc gctggcaaac
    11941 ctgacggcgg caattaacac cacgacttcg aatattcaaa gtttgaatac ggaagagaaa
    12001 gatggtcgcg tctacagcgc ctttattcgt ctgaccgccc gtgaccgtgt gcatctggcg
    12061 aatatcatgc gcaaaatccg cgtgatgcca gacgtgatta aagtcacccg aaaccgaaat
    12121 taatgtttta tgaacccaac acgttatgca cgcatctgcg aaatgctcgc caggcggcag
    12181 cctgatctga ccgtctgcat ggagcaggtc cacaaacctc ataacgtttc tgcgattatt
    12241 cgtaccgcag atgccgttgg cgtacatgaa gttcacgccg tctggcctgg tagccgcatg
    12301 cgcaccatgg cttcggcagc ggcgggtagt aacagctggg tacaggtgaa aacacaccgc
    12361 accattggcg atgccgtcgc tcatctgaaa ggccagggta tgcagattct ggcaacccat
    12421 ctttctgata acgctgtcga tttccgcgaa attgattaca ctcgcccgac ctgcattttg
    12481 atgggacagg agaaaacggg catcacgcag gaagcattgg ccctggcgga tcaggacatc
    12541 atcattccga tgatcggcat ggtgcagtcg ctgaatgttt ccgttgcctc agccctcatt
    12601 ctttacgaag cccagcgtca gcggcaaaat gcaggcatgt acctgcgtga aaacagcatg
    12661 ttgccggaag cagagcaaca acgcctgttg tttgaaggcg gctatccggt gctggcgaaa
    12721 gtcgcaaaac gcaaaggcct gccttatccc cacgtcaatc agcaaggcga gatcgaagct
    12781 gatgccgact ggtgggctac tatgcaggct gcagggtaag tgccatgaaa ggtcgcctgt
    12841 tagatgctgt cccactcagt tccctaacgg gcgttggcgc agcacttagt aacaaactgg
    12901 cgaaaatcaa cctgcatacc gtgcaggatc tactcttaca ccttcccctg cgctacgaag
    12961 atcgcaccca tctctacccc atcggagaac tactgccggg cgtttatgcc acggtggaag
    13021 gcgaagtgct gaactgcaat atctccttcg gcggtcggcg gatgatgacc tgccagatca
    13081 gcgacggttc cggcatcctc accatgcgct ttttcaattt cagcgcggca atgaaaaata
    13141 gcctggcgac gggccgccgt gtactggctt atggcgaagc aaagcgcggt aaatatggtg
    13201 cggagatgat ccacccggaa taccgcgtgc agggcgatct cagcacgcca gaattacagg
    13261 aaacgctcac gccggtttat ccaacaacgg aaggcgtaaa gcaggccacg ctgcgtaaat
    13321 taaccgacca ggcgctggat ctgctcgaca cctgcgccat tgaagaactc ctgccgccgg
    13381 aactgtcaca aggaatgatg acgctaccgg aagcgttgcg cactttgcac cgcccgccac
    13441 cgacgctaca gcttagcgat ctggaaaccg ggcagcatcc ggcgcaacgt cgtctgattc
    13501 tggaagaact gctggcgcac aacctcagca tgttagcctt acgtgccgga gcacagcgtt
    13561 ttcatgccca gccgctgagc gccaatgatg cgctgaaaaa taaactcctc gcagccttac
    13621 cgttcaagcc aacgggcgca caggcacgcg tagtggcgga gatcgagcgc gatatggcgc
    13681 tggatgtgcc gatgatgcgc ctggtacagg gcgatgtagg ttccggtaaa acgctggtcg
    13741 ccgccctcgc cgcgctgcgt gcgattgccc acggcaaaca ggtggcattg atggcaccaa
    13801 ccgaattact cgccgaacag cacgccaata acttccgcaa ctggtttgaa ccgctcggta
    13861 tcgaagtggg ctggctcgcc ggtaagcaga aaggcaaagc gcgactgtct cagcaggaag
    13921 ccatcgccag cggtcaggtg cagatgattg ttggtacgca cgccatcttc caggaacagg
    13981 tgcagtttaa cggtctggcg ctggtgatta tcgacgaaca acatcgcttt ggcgtgcatc
    14041 agcgtctggc gttatgggag aaaggccagc agcaaggctt ccatccgcat cagttgatca
    14101 tgaccgccac gccgatcccc cgcacgctgg caatgacggc gtatgccgat ctcgatacct
    14161 cggtgataga tgaactgccg ccaggccgca cgccagtgac tacggtcgct attcctgata
    14221 ctcgccgtac cgacatcatt gaccgcgtgc gccacgcctg catgactgaa ggtcgtcagg
    14281 cgtactgggt ttgtacgttg attgaagagt cagagttact ggaagcgcag gcggcggaag
    14341 ctacctggga agagttgaaa ctggcgctac cagaattgaa tgttggcctg gtacacgggc
    14401 ggatgaaacc agccgagaaa caggcggtga tggcgtcgtt taaacaaggt gagctacatc
    14461 tgctggttgc cactaccgtt attgaagtcg gtgttgatgt gcctaacgcc agcctgatga
    14521 ttatcgaaaa ccctgagcgt ctgggtctgg cgcagttaca ccagctgcgc gggcgcgtag
    14581 gtcgtggcgc ggtggcttct cactgcgtgc tactctacaa aacgccgctt tctaaaacgg
    14641 cgcaaattcg cctgcaagtg ctgcgcgaca gtaacgacgg ttttgtgatt gcgcaaaaag
    14701 atctggagat tcgcggccct ggcgaattgt taggcacgcg tcagacgggt aatgctgaat
    14761 ttaaagtggc ggatttactg cgcgatcagg cgatgatccc ggaagttcag cgcctggcac
    14821 gccatattca cgaacgttac ccacaacagg caaaagccct gatagaacgc tggatgccgg
    14881 agacggaacg ttactcgaat gcgtaaaagg cggcagtgag aagaccgcca tttcaggtta
    14941 ccctaccttc ctgccggatg cgattcatca ccctacaaat tcaataaatt atgaatcaat
    15001 acgcaggctt gataagcgaa gcgtatcagg catttgtctt tgttgcggcg gcacttctac
    15061 cgccgcttca tcggttaacc ggcaaaaatc ggcaacatca aatacaactt aattaccagc
    15121 gcattgacga tatcgataaa gaacgcaccg accatcggca ccaccaaaaa cgccatgtgc
    15181 gacgggccaa agcgttcagt aatcgcctgc atgttggcga ttgccgttgg cgttgcaccg
    15241 aggccaaaac cacagtgacc tgcagccagc actgccgcat cgtagttttt gcccatcatg
    15301 cgccaggtaa cgaagatggc atacaacgcc atgaagatgg tctgtaccac cagaatcgcc
    15361 agcatcggca gcgccagcga agccagctcc cacagtttca gccccatcaa cgccatcgcc
    15421 aggaacaacg acaagcttac gttacccagc acggataccg cacgctcaaa gacgcggtaa
    15481 aagcccatta ttgacagacc gttgctcaga atcacgccaa caaacagtac acagacgaag
    15541 gtcggcagtt caaaagcagt gccagccaaa agttgcgcaa caattttccc caccgtcagg
    15601 cagatagcaa tcagcgcgat agtttcaatc agcaccaacg aggtgatcat gcgtcccaca
    15661 tccggctttt caaacgccgt cgggacttcc tggtcatccg gaataccgtt cggcgtggtg
    15721 gagtgtttca ccagatagcg cgccaccgga ccgccaatca agccgcccag caccagaccg
    15781 aacgttgcac aggccatcgc cacttccgtc gcattggtga agccataacg ttcaatgaac
    15841 aatttactcc acgcagcgcc cgtaccgtga ccgccggaaa gcgtaataga accggccaac
    15901 agccccatca gcggatcaag ccctaacagg ctagccatac caatgccaat ggcattttgc
    15961 atcaccaaca gaccaacaac cacaatcaag aagatgccaa ccacacgccc accggcacgc
    16021 aaactggcaa tgttggcgtt caggccaatg gtggcgaaga aagccagcat taacggatcg
    16081 cgcagggaca tatcaaagtt gacttcccag cccatgcttt ttttcagtac tagtagcgcc
    16141 agcgccacca acaaaccacc cgcaacaggt tccggtatgg tgtatttctt caaaaaggag
    16201 acggaatgga ccaacttacg cccgagcagc aacgtcagcg ttgcggcaac aagcgttgct
    16261 aaagtatcga gatgaaacat agttactcct ttgtatccgc atcactcttt catactcgtc
    16321 atacttcatc gcgcagtggc aacttcatga actatgacgg gtatcgagtt atgctttcct
    16381 gggtcaaatt ctcagctgtt aatgaacaaa tggcatggat tttaagcaga aaaataccaa
    16441 aagttatata aaaaagtgca tttatctcat ttaaatattt agatgaggat gtggcaaacg
    16501 tttgcttttc tctttcggtc agctaaaatg cccgctttgc taccacggga ttgttttcga
    16561 tgtctgtttc caccctcgag tcagaaaatg cgcaaccggt tgcgcagact caaaacagcg
    16621 aactgattta ccgtcttgaa gatcgtccgc cgcttcctca aaccctgttt gccgcctgtc
    16681 agcatctgct ggcgatgttc gttgcggtga tcacgccagc gctattaatc tgccaggcgc
    16741 tgggtttacc ggcacaagac acgcaacaca ttattagtat gtcgctgttt gcctccggtg
    16801 tggcatcgat tattcaaatt aaggcctggg gtccggttgg ctccgggctg ttgtctattc
    16861 agggcaccag cttcaacttt gttgccccgc tgattatggg cggtaccgcg ctgaaaaccg
    16921 gtggtgctga tgttcctacc atgatggcgg ctttgttcgg cacgttgatg ctggcaagtt
    16981 gcaccgagat ggtgatctcc cgcgttctgc atctggcgcg ccgcattatt acgccgctgg
    17041 tttctggcgt tgtggtgatg attatcggcc tgtcgctaat tcaggtgggg ctgacctcca
    17101 ttggcggcgg ttacgcagcc atgagcgata acaccttcgg cgcaccgaaa aatctgctgc
    17161 tggcaggcgt ggttttagcc ttaattatcc tgcttaaccg tcaacgtaac ccttacttac
    17221 gcgtggcctc actggtaatt gcgatggcgg ccggatatgc gctggcgtgg tttatgggca
    17281 tgttgccaga aagcaacgaa ccgatgacgc aagaactgat tatggtgcca acgccgctct
    17341 attacggtct tggcattgaa tggagtctgc tgctgccgct gatgctggtc tttatgatca
    17401 cttcgctgga aaccattggc gatatcacgg cgacctctga cgtttccgaa cagccagtgt
    17461 ccggtccgct gtacatgaaa cgcctgaaag gcggcgtgct ggcaaacggc ctgaactcgt
    17521 ttgtttcggc ggtgtttaac accttcccga actcctgctt cgggcaaaac aacggagtga
    17581 tccagttgac cggtgttgcc agccgctatg tcggttttgt cgtcgcgctg atgttgatcg
    17641 tgctgggtct gttcccggca gtgagcggtt ttgtacaaca cattccagaa ccggttctgg
    17701 gcggcgcaac gcttgtaatg tttggcacca tcgccgcctc cggtgtgcgt atcgtttctc
    17761 gtgagccgct gaaccgtcgg gcgattctga ttatcgcgct gtcgctggcg gttggtctgg
    17821 gcgtgtctca gcagccgctg attttgcagt ttgcccctga atggctgaaa aacctgctct
    17881 cctccgggat cgccgcgggc ggtattactg ccatcgtgct gaatctgatt ttcccaccag
    17941 aaaaacagta attcactggc ggcggtaaga taattaccgc cgcagtctca cctctttaac
    18001 cattcccgcc ttgaggattg cgcgtaaatc gtgcataact cccttatgtg catttcacgg
    18061 gatggaagac catgaaattt attgggaagc tgcttctcta cattctcatc gctctgttag
    18121 tggcgatcgc tggcctctat tttcttctgc aaacccgctg gggagcagaa catatcagcg
    18181 catgggtttc cgagaatagc gactatcatc tggccttcgg ggcgatggtt caccgttttt
    18241 ccgcgccatc tcatatcgtg ctggagaatg tcacgtttgg tcgtgatggc cagcccgcga
    18301 ccctggtggc caaaagtgtc gacattgcgc taagtagtcg gcaactgacc gaaccacgcc
    18361 atgtcgatac catcctgctg gaaaacggga cgctgaatct caccgaccag accgcgccgc
    18421 taccgttcaa agccgatcgt ctgcaactgc gtgatatggc gtttaatagc ccgaatagcg
    18481 aatggaaact gagcgcgcag cgggtaaatg gcggcgtggt tccgtggtca ccagaagccg
    18541 gtaaagtgct gggtacgaag gcgcagattc agtttagtgc cggatcgctt tcgctcaatg
    18601 atgttcctgc caccaatgta ctgattgaag gcagtattga taacgatcgc gttacgctga
    18661 ctaacctggg tgccgacatc gcgcgcggga cattaaccgg aaacgcgcag cgtaacgccg
    18721 acggcagctg gcaagtggaa aacctgcgca tggcggatat ccgtctacaa agcgaaaaat
    18781 cgctaaccga cttctttgcg ccattacgct ctgtcccgtc gttgcagatt ggtcgcctgg
    18841 aagtgatcga tgctcgtttg caaggtccgg actgggcggt gaccgacctc gatctcagct
    18901 tgcgcaacat gaccttcagt aaagatgact ggcagacaca agaaggcaaa ctgtcgatga
    18961 acgctagcga gttcatttat ggttcgctgc atttatttga cccgattata aacgcggaat
    19021 tttctccgca gggcgtagcg ctgcgccagt tcaccagccg ctgggaaggg ggtatggtca
    19081 gaacgtcagg gaactggctg cgtgacggga aaacgttgat ccttgatgat gcggcaattg
    19141 ccggactgga atataccttg ccgaaaaact ggcaacagtt gtggatggaa acgacacccg
    19201 gttggttaaa cagcctgcaa ctgaagagat ttagcgccag ccgcaatctg atcattgata
    19261 tcgaccctga cttcccgtgg cagctcaccg cgctcgatgg ttacggtgcc aacctgacgc
    19321 tggttaccga tcataaatgg ggcgtctgga gtggctcggc gaatctgaat gccgccgccg
    19381 cgacgtttaa tcgtgttgat gtccgtcgtc cgtcgctggc gctaaccgcc aacagcagca
    19441 cggtgaatat cagcgaactg agtgcattta ctgaaaaagg cattctggaa gccaccgcca
    19501 gtgtttcaca aacgccacaa cgtcagacac atatcagcct gaatggacgc ggtgtgccgg
    19561 tgaatatttt gcaacagtgg ggatggcctg aattaccgtt gactggcgac ggcaatattc
    19621 agcttaccgc cagtggcgat attcaggcca atgtcccgtt gaaacctacg gttagcgggc
    19681 aattgcatgc cgtgaacgcc gcaaagcagc aagtgactca aaccatgaat gcgggcgtcg
    19741 tttccagcgg tgaagttaca tcggcagagc tggtgcagta aacatcaatc tcgccgggta
    19801 cgactcagac gtacccggca ttcttcaata gattacaacg taattgtcag cgcattccct
    19861 tgaggcgtaa ccaccagccc ctgctcactt tcagcctgcg aaccgccttg cagaccattt
    19921 actttcacaa tattgcgcaa gcacagtgtc cagttcctcg cctcgcccgc accagtcaca
    19981 gtaatcgtgt taccagtacg tgttgctttt agagtaaaga tcaccgaacc gtcagcagcg
    20041 ggcacttcac atacagcttc atgcccgtct tgcagattaa agagatggaa tgccgtgcct
    20101 tcgtgccacg cgtaatcggg acgttgatcg ttgttgccca gcgccaatag ggtgttatca
    20161 cgcacataaa cgggcagact caggaagccg tgctgctgtt tatgccagcg actaccgtcg
    20221 agctcattgt tgtgccacag gtgtgtccag cgaccttccg gcaagtagaa ctgcacctcg
    20281 cccgcctcag tgaacaccgg agcaaccatc acgttgtcgc ctagcatgta ttgacggtca
    20341 aggtaatcac aagccggatc gtccgggaac tccatcatca tggcccgcat catcggcgta
    20401 ccccgcgctt tcgcacgcgc agcttcacga tacagatacg gcatcatgcg gcatttcagt
    20461 tgcgtgaaga agcgcaccac atcacaggac tcatcatcgt aggcccacgg cacacgataa
    20521 gatttgctgc catgtaaacg actatggctg gagagcaaac caaacgcgca ccagcgtttg
    20581 taaacgtgtg ccggcgcggt attttcaaag ccgccgatat cgtggctcca gaagccaaaa
    20641 cctgaaaggc caatagacaa accaccgcgc aggctttccg ccattgattc gtagttagcg
    20701 taacaatcgc caccccagtg taccgggaat ttctgcgcac cgacggaggc cgagcgggca
    20761 aacaagacag cttcttcctc accaacggtg tccttgagca cgttccacac cagttcgttg
    20821 tagatgtacg cataatggtt atgcattttc tgcggatcgg aaccgtcaaa ccactgaaca
    20881 tcagttggga tacgttcgcc aaagtcggtc ttaaagcaat caacgcccat cgcgaccaga
    20941 cctttcagtt tgtcggcgta ccatttgcag gcatccggat tggtaaagtc ataaatcgcc
    21001 aga
//
LOCUS       DAJTZL010000067        20711 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.067, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000067 DAJTZL010000000
VERSION     DAJTZL010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20711
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.067"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(73..4533)
                     /gene="mukB"
                     /locus_tag="OO544_002089"
     CDS             complement(73..4533)
                     /gene="mukB"
                     /locus_tag="OO544_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HCR6767687.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTAQRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     EQEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNVVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPSQES"
     gene            complement(4533..5237)
                     /gene="mukE"
                     /locus_tag="OO544_002090"
     CDS             complement(4533..5237)
                     /gene="mukE"
                     /locus_tag="OO544_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HCR6767688.1"
                     /translation="MSSTNIEQVMSVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEENQPDSGEEE"
     gene            complement(5218..6540)
                     /gene="mukF"
                     /locus_tag="OO544_002091"
     CDS             complement(5218..6540)
                     /gene="mukF"
                     /locus_tag="OO544_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HCR6767689.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(6537..7322)
                     /gene="cmoM"
                     /locus_tag="OO544_002092"
     CDS             complement(6537..7322)
                     /gene="cmoM"
                     /locus_tag="OO544_002092"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HCR6767690.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            7458..8237
                     /gene="elyC"
                     /locus_tag="OO544_002093"
     CDS             7458..8237
                     /gene="elyC"
                     /locus_tag="OO544_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HCR6767691.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTAKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEELNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(8214..9107)
                     /locus_tag="OO544_002094"
     CDS             complement(8214..9107)
                     /locus_tag="OO544_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HCR6767692.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(9261..10007)
                     /gene="kdsB"
                     /locus_tag="OO544_002095"
     CDS             complement(9261..10007)
                     /gene="kdsB"
                     /locus_tag="OO544_002095"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HCR6767693.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(10004..10186)
                     /gene="ycaR"
                     /locus_tag="OO544_002096"
     CDS             complement(10004..10186)
                     /gene="ycaR"
                     /locus_tag="OO544_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HCR6767694.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(10238..11470)
                     /locus_tag="OO544_002097"
     CDS             complement(10238..11470)
                     /locus_tag="OO544_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaQ family DNA glycosylase"
                     /protein_id="HCR6767695.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(11507..12493)
                     /gene="lpxK"
                     /locus_tag="OO544_002098"
     CDS             complement(11507..12493)
                     /gene="lpxK"
                     /locus_tag="OO544_002098"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HCR6767696.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(12490..14238)
                     /gene="msbA"
                     /locus_tag="OO544_002099"
     CDS             complement(12490..14238)
                     /gene="msbA"
                     /locus_tag="OO544_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HCR6767697.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DNLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAIDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(14275..16539)
                     /locus_tag="OO544_002100"
     CDS             complement(14275..16539)
                     /locus_tag="OO544_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
                     /protein_id="HCR6767698.1"
                     /translation="MKITTVDVCIICGIFPLLILPQLPGTLALAFLTLFACVLAFIPV
                     KTVRYIALTLLFFVWGILSAKQILWAGETLTGATQDAIVEITATDGMTTHYGQITYLQ
                     GRRIFPAPGLVLYGEYLPQAVCAGQLWSMKLKVRAVHGQLNDGGFDSQRYAIAQHQPL
                     TGRFLQASLIEPNCSLRAQYLASLQTTLQPYPWNAVILGLGMGERLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAVLLAAGLIRSGQIFLPGRWIHWQIPLIGGICCAAFYAWLTG
                     MQPPALRTVVALATWGMLKLSGRQWSGWDVWICCLAAILLMDPVAILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLTSFIANL
                     LAIPLVTFITVPLILAAMVVHLSGPLILEQGLWFLADRSLALLFWGLKSLPEGWINIA
                     ERWQWLSFSPWFLLVVWRLNAWRTLPAMCVAVGLLMCWPLWQKPRPDEWQVYMLDVGQ
                     GLAMVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEHLDHRGGL
                     DSILHTWPMLWIRSPLNWEHHQPCVRGEAWQWQGLRFSVHWPLQASNDKGNNHSCVVK
                     VDDGTNSILLTGDIEVPAEQKMLSRYWQQVQTTLLQVPHHGSNTSSSLPLIQRVNGKV
                     ALASASRYNAWRLPSSKVKHRYQQQGYKWLDTPHQGQIAVNFSAQGWRISSLREQILP
                     RWYHQWFGVPVDNG"
     gene            complement(16746..17030)
                     /gene="ihfB"
                     /locus_tag="OO544_002101"
     CDS             complement(16746..17030)
                     /gene="ihfB"
                     /locus_tag="OO544_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HCR6767699.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(17190..18863)
                     /gene="rpsA"
                     /locus_tag="OO544_002102"
     CDS             complement(17190..18863)
                     /gene="rpsA"
                     /locus_tag="OO544_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HCR6767700.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(18974..19657)
                     /gene="cmk"
                     /locus_tag="OO544_002103"
     CDS             complement(18974..19657)
                     /gene="cmk"
                     /locus_tag="OO544_002103"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HCR6767701.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPASDALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            complement(19830..20594)
                     /gene="ycaL"
                     /locus_tag="OO544_002104"
     CDS             complement(19830..20594)
                     /gene="ycaL"
                     /locus_tag="OO544_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HCR6767702.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
BASE COUNT         4970 a         5732 c         5045 g         4964 t
ORIGIN      
        1 aatgtgccac ttttttagtg cagaaacgga gttttcggaa aaagaaaagg cggcattgct
       61 gccgccttaa ttttaactct cctgagaagg cgcttcgtca cttcctggaa gcgtttcagg
      121 gagttgcggc gcaaatcctc gcaggccgac gacgtgaacg tgttcggtat tctggaagac
      181 tttacgcacc agtttatagg tggtgccttt ctccgggctg atattttccg gcgctgcgat
      241 gatgagctgc atttgcaaac gttcacacaa ttcaaacagc gtggcgatag aacgagcatc
      301 cagtcgcgct gcttcatcga ggaacagcag gcggcaagga gagatatctt taccgcgcag
      361 gcggcgagat tcatcttccc agctttgtac caccatcacc agaatcgaca taccggtacc
      421 aatcgcctca ccggtcgaca atgcaccaga ctctgcgcgc aaccagccat cggaaccacg
      481 gttaacctca acttccattt ccagatagtt gcggtaatcc agcagttctt cgccaatggt
      541 ctgcggcgtg cgctgcccca tatcaatctg cggattaaga cgctgataca atttcgccag
      601 cgcttccgag aaggtcagac ggttgctgtt aaacagatcc tgatgctgct cgtgctgttc
      661 agagagcaca tccagcagca tggcgtgcgt ttcacgcacg ttcacgttca gacgtacgct
      721 gttcacctga ccaaacgata cgttctgcaa cccctggttg agcatacgga tacggttctg
      781 ttcgcgctga atggttttgc gaatgatgtt cgccacgctg cgggaactga tcgccagttt
      841 ctgttcgcgg gaggttaact cctcggtcag tcggctaagt tcgatttcca tctgctcgat
      901 ggcttccacc ggatcatcgg tacgaataat atcctgacga atacgttcac gcagatgctg
      961 ataaaccgcc acgaagaact gaattttacg ttccggacgt tttggatctt ccgacatgcg
     1021 cagcacgtcg cgcagatgtt cgttatccgc caccgccaga cgcagcgcac ccaatgcctt
     1081 atccgacata gagcgcaaat catcagcgga gagataagcc agctcgcgac ggtgtaagcg
     1141 ccgttcaacg acgttatctt tcaccatgcg catcactgca caccagcccg ctttggcggt
     1201 cactacctgc tcgcgcatct caaagtaatc ccgctccagc ttgcgcagtt tgcgggtcag
     1261 attgtccatc tccgcttcgc agaaagtcag tgctttttcc agctggttgc ggcgggaccg
     1321 gttattactc agttgcgcat gcagctcgtc acggcgaatt cgcgcccgct cttctgcccc
     1381 gctatcggcg cgcacgccga tatcctgcaa ttcacgttgc agatcgttga gtagctcttt
     1441 tttggtgtcg taagaacttt tcagcgaagc cagcacctgg ttgtactgac tcaactgcgc
     1501 tgcgtgacca cgcaacgctt cacgagcgcg agtacgctcc gcttccgcct gttccagacg
     1561 ttcacgcagc ttttcgttga gatcgctatt accgctcagc atttctgccg agtcagaata
     1621 gctaaagtgc gcacgacgct gcaccacttc cgtcagggca aacgcctgct gacgggcatc
     1681 gcgctgcatc tgctgagagt acgcgtaatc ttcttttagc tgttcgaact gttccgggtc
     1741 gctctgcaat accgaaacga tcggttccag tttcgccagt tgattgccaa actgctgaac
     1801 aaaacgcgcg gcttcctggg cttcatccag acgttcgcgg atttcatcga cgcgatccgc
     1861 caggctgtca tcagccaaca ggttgagacg cggcagaatg cggttcagcg ccgtaacgcc
     1921 ttctttcgcc tgctcaaact gaatacgctg ctgctggtta tcattttcat gattactcaa
     1981 cgcccgctcc agttcgacgc gacggctgtt cagttgacgg atttctgctt ccgggtcaga
     2041 ctcaaacgcc accgccagat gactgccgat aaagcggctg aacgcctgat gcagacgctg
     2101 ggttttctgt acatcaaagg agagcgtggc gaagcgttcg gaaagcactt cacgctcggc
     2161 atggaggctt tcaatacggc tttcacgcgc agcacgacca aacagcggca cttccgggaa
     2221 acgtgaataa cgccactgac gatcggcgat tttcaccact accgcttttt ccagctcatc
     2281 aacgctgaac acgctgtcat cgaatgactg cggatcccct tcgatcaaat agagatcttc
     2341 cgggcaatcg gtcaagcctt ccaggtgttc agttacctgt gacagatctg gcaccacgat
     2401 ggcgtggcgt gacgggccat acagcgctga gaagtacggc gcatcttcca ggctaacgtc
     2461 gtcataaatt tctgacagca gcacgccgcc aaaacgctcc gccagcgcgt tcagacgctg
     2521 atcttcagag ccgccaggct ggcttaaacg ttcgatctct tcatcgacag cgtttttgcg
     2581 cgcgcccact tcatcgcgtt caacaatcgc ctctcgctca cgctccagca actgttgcag
     2641 atattcggtg acgtcctggc tggaggtaaa ctcttcaccg cactgctcgc tcaactggtt
     2701 gagactgttt tgcgctgcca gccaaaccgg cgcacgctgc atcaaactct gaatgcgaga
     2761 ctgcagctgt tcctgttcct ggcgcagtgc catgcgctct tcacgggcgt tagacacgct
     2821 atcggaaaga gaggcaatgc gcgcttccag ctcctgatgc agagcttcca gttcatcaat
     2881 atcaaaattc ttgccctgac gtttgcagaa gtctgccagc aggcgttcgg cttcttgctg
     2941 ctcgcgcaga cgctgttcca gttcacttaa gcgcatccgc aacggttgaa cctgctctgc
     3001 caggtgacgc tgatcgaccc cttcgcgcag cagttcgcgg gcgacatccc aagcctcgtt
     3061 acgcgccagc gggccgttga ttgccaccac cagctgataa gcctgctcaa actggctgtg
     3121 cgcggtttgc gccatgctca ttttctgctc aagagagagc attttttcag tcgcttcctg
     3181 ctctttcgcc tggaaggttt ccagccattc ggcggcgctg tcggcggtta agtccggcag
     3241 atggcacagt tctttggcgc gattaagcgc agcaatcgcc tggttatact ggatcgcgcg
     3301 cgtttgctgg acgtccagcg cctgctggta gtcagcaagc tggcttttca gctcgtccac
     3361 ttccagttcg gcagcttccg cgcgagcctc gttctcttcc tggcgttcga tggcttctgc
     3421 caccacttcg ttttgctctt ccagacggat ctgcagctca tcgagatccg cttcgtagcg
     3481 ttcgatcttc tcctgctgac gctgtgcggt ttgcaccagg ttcaggtgat cgctggccgc
     3541 ctgataatcc gcttccagat cgccttcggc accgttgtgc tctgccagct cacgcgccat
     3601 atcgacgtgt ttgtactgct cagccgccag ttgctgacgc gaagtatgta actcgcgacg
     3661 aaactccagg gctttgtcga gatggacacg gcgctcgttg gcgtgacgca tgtagtccgc
     3721 cgccacgtag ttggtagctt cgctgatcag atgcttaaac aggtcacggt ccgactgggt
     3781 gacacgaatc gcttccagcg tcatgcggtt ttcacgcagc gccgcttcca tgtcctggaa
     3841 cgctttacgc acgccgctgt tttccggcaa caggtagtcg cgcagagaac gggtaatggc
     3901 actggagatc ccgccataca gcgaagcttc gatcagacga tagaatttgc tacggtcaga
     3961 tgcggagcgc agacgacgcg cgatgatgcc cagatcgaac atcagcgagt ggtaatcggt
     4021 aatggagttg aactgtttaa actgcacgcc ttccatcgcc tcgagcttgt ctttcagctc
     4081 gttaagcggc agcacgcgcg cctggcgttc gttcagggtt tcggtcacca gctgtgtcgg
     4141 ctgcaccgac atcggcagtc cctgaatggc aaatggtttg atatccactt tacgatcgcg
     4201 tccggcaacc tgttgcagac gcacaccgac caccacgcgc tggtggcgcg agttaatggt
     4261 gtcgagcatc gaataacaga cacccgcttt cagcttaccg tgcagacctt tatcgcgcga
     4321 accgctggtg gccccggctt ccgtagtgtt acggaaatgc agcagggtca ggtcggggat
     4381 cagcgccgta acgaacgccg ccatggtggt ggatttaccc gccccgttac cgccagaaag
     4441 cgtcgtgacc agctcgtcaa ggtcaaaagt tcgggcaaaa aagccgttcc agttaatcag
     4501 cgtcagtgag cgaaatttac cgcgttcaat cattattctt cctctccgct atctggctga
     4561 ttctcttcgg tttcatcgtt gagttgcaga tgattttcaa tcggcattgc ttcgccatca
     4621 cgaatcaggc gacgctgtgc ttcacgggga tcgtcgccag cacgcacatc ggctccgaag
     4681 cggaacaccg attcggtaat gcgaaacttg ctgctgtcgt ggcccataaa ccacaccatg
     4741 cctaaacgac gcagacggtt gagcgaagaa cgtactttct cctgcaactt ctgacggtca
     4801 acgtctgaac cggttgaacg gttgttcacc agtttcagca gttttgcttc atcggccagg
     4861 gtgagcagtt cgtcgtacag ttcctgctgg gtgaaaatcc cctcattcgc cagccgttcc
     4921 gggctgagat agagataaca gaggattttc ccgaccatca tatccagttc cgacaagacg
     4981 gaacgcggga taagagtggt ggaacgtggg cgtaaataga agaacccttc cggtgcgcga
     5041 ataagctcaa cgttataacg cgcgtaaaac tcttccagat attcctgaaa atccatcagg
     5101 aatgcatgat tatccagttc gtcgaggcca atatggcggc ctgaacgtaa ggcgctgtcc
     5161 agcgccggaa ataacggatt cgccagcgcc tgcgccagct taaccgacat cacttgttca
     5221 atatttgtcg atgacatgcg cctgtacctt ggctccgtaa tcattaatcg gctgccattt
     5281 cgctggcagt ccggtgaaat ctgcttgcgc tacgccaaga cgtaccgcct gatcaataac
     5341 aatacgcgca acgtcaaagt gacgtgcacg cggatactgt gacagatatt cgcgtaccac
     5401 cagaccaaga tccagcggca cttgtctggt tttgtacacg gcaagttgtt cttcgatgat
     5461 cgccgccagc tgttcgcgga tctcgttaaa ctcttcgtat tccagatcct caggaagttc
     5521 cccagtcact tcttcatcgc gcagtgccat ctcttcgtca cgcatatcca gcagacgatc
     5581 ggcattggca taagttagcg cccacggctc atcaaaatag gtttgtaccg actgacgtaa
     5641 ccgctgagca aagacgcggt ttttatccat atcgatcgcg gtacgaataa atttgtgtac
     5701 gtggcggtcg tagccaatcc acaagtcgat ggattgctgg ccccaactga taatacgatc
     5761 gagtttgctc tgcagatcga acaccagacg atcgacgaaa tgcagatcgt catgggtcat
     5821 cgtcgcatcc tgaatgcgca acagattagc ctgcaatttg tcgcctgccg cttccagcgt
     5881 atcctgcaat tcacgcagcg ttccggaagt ttccgaaagc aacaattcac agctggaaat
     5941 cgccgcccgc cagtctttgt tcagcaactg ggcgatatcg tccttcacct gctgctgctg
     6001 ttcgtccatc agacgttgcg tcaggtcgat actgtcgaaa atttctgcta ccgaatattt
     6061 cagtggcgca tagacattac ggtgccagtg aaattcatca ccgccctctt cggcggcatc
     6121 tgctgcgcgt ttgagctcac ccgccacaat cgacaactgc atagaaagac gcagcgtaga
     6181 aaactcgcgc tgacggatgt agtagtcagt aatgccgatg ccgagcggcg tcagacggta
     6241 aattgcgttc ccttccgcct gctcgctggt aaagcggttc agcagacgtt gacgcaccat
     6301 gtcgttgatc gcgttattgg cgcgcacgcc gatggtttcg ctggtttgct caaacgcatc
     6361 actcacatgg cggaatgcat ccaccagctc gccttcactc atctcaccat ccagacgctc
     6421 gccgttcagc gtggcaaccg ccagcagaaa agagagtcgg tctaccggca gcgagatgga
     6481 gaagtcattt tttctggccc aggcaaccag ttcggggact gtctgggaaa attcactcat
     6541 actttatcct tgctctgcgg tttgcgcgcg gtgacatgaa tataacgccc cagggtaata
     6601 tacggttcct gacggcaata acgcgtttct aattcaagta atgcttcata gcagtcgcgc
     6661 tgctggtgct tctcgcgcag ataatcatga aacacgcgaa cgcctgtctt acccataatt
     6721 tgccaaccag cttcttccag ccacagataa acctgcgccg ggtcgcgtgg ataatctggc
     6781 gaaagcgtcc gttttttctt tttcggcatt cccgcctgca cgtaatcaaa attcccggcg
     6841 accatgttat gcatcaacaa accatgcgca ttgtagaaca ttaacgacaa cacgccgcct
     6901 ggacgcaaca ctgaccacag ggtctgcaat acactgcggg gatcggccac ccactcgagc
     6961 accgcatgga acaatatcag atcaacgggc gtttccaaat gcgaagcaac atcctgagcg
     7021 gcgcaatgta taaattgcat gttgtcgctc acaccttttg cttctgccgc ctgttttgcg
     7081 cggtcgatca tctgcgcaga aagatcgcat aaaatgacct gatgcccgcg ctcggccatt
     7141 ttgattgcgg tctgcccttc tccaccgccc gcatccagca cacgcagttt ttgcggaccc
     7201 atttccgcca gcacgcgatc gagatcctgc cacagaatag cctgtcgaag ctgccctttg
     7261 gtggtgccgt aaatgttacg ggaaaacttt tccgcaatat catcaaaatt gcgatcctgc
     7321 attgctttct ccaccggttg gctaagacgt tttctgataa agacgctatt ttgtcacagc
     7381 cacggcgaga atgaacccgt ctggtgtaat atcgctggcg atcccttgct atatggttaa
     7441 aaaaggaacc agaaaggatg ctttttacac tgaaaaaagt gattggcaat atgttgctgc
     7501 cacttccgct gatgttgtta attattggcg ctggcctggc gctgctgtgg tttagccgtt
     7561 ttcaaaaaac cgcgaagatt tttattagca ttgggtggct ggcactcttg ttattgagtt
     7621 tgcaaccggt agccgaccgc ctgctgcgtc ccatcgaaag cacctatccg acatggaata
     7681 attcgcaaaa agtggactat atcgtggtgc tcggaggtgg ttatacctgg aacccgcagt
     7741 gggcaccgag ctctaattta atcaataaca gtctgccacg cctgaatgaa ggtattcgct
     7801 tatggcgcga aaatccggga tcaaaactga tcttcacggg aggcgtagca aaaaccaata
     7861 cggtgagtac agcggaagta ggtgccagag ttgcgcaatc gctgggcgtg ccgcgcgagc
     7921 aaattatcac cctggatttg ccaaaagata ccgaagaaga agctgcggca gtgaaacagg
     7981 cgattggtga cgcccctttc ctgctggtga cctccgcatc acatctgccg cgggcgatga
     8041 tcttttttca gcaggaagag ttaaatccgc taccagcccc ggcgaatcag ttggccatcg
     8101 actcgccgct taatccgtgg gaacgggcaa tcccttctcc ggtctggctg atgcatagcg
     8161 atcgtgttgg ctatgaaacg cttggccgca tctggcagtg gctgaaaggt ccgtcaggcg
     8221 agccacggca agagtgattt tgatgccaga tcgaagttgc gccgactaaa tcgtccggta
     8281 ttaaccagtt gcgcaacttc atcccataac acatacaacc aacgccgcca gatgaacgac
     8341 tccgccactg gcgcgcgttg caggtaactc cagagcaaat cttccgccag agaattatcc
     8401 attagtcgga acagttcgta ttctcgcggt gcccaaagca ttagtcccgg cccgaccatc
     8461 gccagtaact gatcgctgcg cgaatctttc agcatgctgc gtaaacagaa gttaccgtga
     8521 atcaacacac aattgtcgtt aaagccttca aataatgccg ggagacattc gcgagtgcga
     8581 aacaggatcc gcttatcctg catcgtcaga ccggtgttat tgaactgatt gagcgtggtc
     8641 catagcactt caacatgttg ccggtaccat gagggccaga aattttcctg agtattgtcg
     8701 accgcgccga cgcaaccgcg actgtcctga cggtgccagg ccagtaaggc ttcaacgatt
     8761 tggtctttga gctgttccca gcgttctggt gttcgggctg gtgcctccac cgaaacacca
     8821 cgcatccgct ccagtagcag gacatcaggg ccggggtgtt cttcatgcgt catcacacca
     8881 taaatagtcg gcatacggac agtcccactt cttgccagca tggtggtttt ccatgccagt
     8941 tgtcgggctt ttccgggcgt actaaagctt cttgccatta acggcattgg gtttccctgg
     9001 ctgtcataca aagcccacaa cgccgtatcc gctttttcat tgacgcactc aatacgactg
     9061 agtttttcgc ccagtaaatg gcttaattcg gcacgcagct gttccattca gattaccctc
     9121 ataaaagcta ctgctttcat aatgagcgcc aatataaaag atgtcacctg aatgagatca
     9181 attgttgaaa agaaaaattg aggaaaatcg taacggtacg acactcctcc caaaattggc
     9241 tgaagtgtcg tgaagtgaaa ttagcgcatt tcagcgcgaa cgcgctcaag atcttcaggg
     9301 gtatccacac ctgtgccagg aacttcctga gcaacagcaa catggatttt ttcgccgtac
     9361 cacagaacac gaagctgctc taacatttcg atgtgttcta acggacttgc ctgccagttg
     9421 acgtaacgac ggataaagcc tgcacggtag ccataaatac caagatgacg caggaagtta
     9481 tcgccaacgg tttcaaggcc ttctgcaaaa cgatcacgat cccaaggaat ggtggcgcgt
     9541 gagaagtaca gtgcataccc ttcagcgtcg agaaccactt tcaccgcatt cgggttaaac
     9601 gcttcttccg cattgtggat tggcaccgcc agagtcgcca tacccacctg acgctgagcg
     9661 aggttatcag caacctgacg aatgattgtc gcagggatca tcggttcatc accctgcaca
     9721 ttaacgatca ccgtgtcgtc gctgaatgcg catttttcga caacttccgc cagacgttct
     9781 gttcctgact gatgatcggc gcgcgtcata catacttcac cgccagcggc ttcaacggcg
     9841 cgggcaacat cctcatgatc ggttgccacg atgatgcgct cggcacctga ttcacgcgcg
     9901 cgttcaagaa catgaacaat catgggtttg ccgttaatat caaccaatgg tttaccgggc
     9961 agacgcgtgg acgcgtagcg cgcgggaata atgaccacaa aactcatgat ttactctcat
    10021 cagcagtcag cacgcgggct tccgtttcca gcaacactgg aatgccatcg cggagcggga
    10081 aagccaggtt atccagtttg caaataagct cttgtttttc ctggttatac caaagttttc
    10141 cgttgcaaac cgggcaggca atgatttcaa gcaaacgatg atccatagtt cctccagatg
    10201 gatcgggtta tgaatgcata aatcttatca taatcattta tgcgacgggg tctatttccc
    10261 agccagtgcg gcaatcagta aagagacctt gcgggcagcg tcctaatgtc acccgcgttg
    10321 cctgctgcca gctcgcgaaa tcagtaatcg cctgacgtaa ccctttttgc agcattgtcg
    10381 ttggtttaat gccttcctgt aaccacagag agataacttc aaggatgcct gtctggcgat
    10441 gcattttggc atccattcgc ccaactaatt gcccacgatg taataatggc agaacaaaat
    10501 agccatactg gcgtttcggc gctggggtat agcactccag ccggtagcta aaatcaaaaa
    10561 gctgctctgc gcgtttgcga tcccagacaa caggatcaaa aggcgaaagt accgcgctgt
    10621 gcgttgcagt gagctttccg gccagcgcac gctcgagtag cggcagcaaa tcagcatgca
    10681 gccaaagatt gcccaatttt tcaacatgca cagcaatgat ttgctgctgt tcagccctcg
    10741 cttcgcgcca cgccgccagt gccggacgtt tcagccgata gtaatctgcc agccactgtt
    10801 cgcggaatat tcccaagcta cgcgcactgt tatccagcat gatgatttct gcttctgttt
    10861 gcgaaacgag atcgcgctca tcatcccagt caggcatgac acggtgggtt aaatcataaa
    10921 cgcgctggaa gttgcgccgt tcaatcacca tcacctttcc ggcagtaaat aaaccttcca
    10981 gatgccgttt atgcggcttc cattcccacc agccgcttgc acctttacga ggatgctcaa
    11041 aatcggctga acgtaccggc cccttatcat gaatatgctg aattaactgt gcaatttccg
    11101 cctcatgttc ctgcatccag gcgtctttgt atttccagcc cattttttca ggtgccagca
    11161 tgcggtggcg aataagacga aagtcgctac gcggcataaa gcaggcttca tgcgcccagt
    11221 attccattaa ttcgccacgc gccagagact catccagcca ctgggcagga taatttccca
    11281 gacgactgaa aagcaccaga tatggactac gggcaacaat attgatggta tcgatttgca
    11341 gcaaggacat gcgggagatc gttgccggaa tatcctccaa cgacgctcga cggcggggtt
    11401 tgtttaacag gccttgtgcg gcaaggtgaa gattacgcgc atcagcaagg gagaggtgcg
    11461 gcagtgacat tcatgactcc atcaatcgaa cgctgccgcg gcgtaactag ttgccagaag
    11521 ccagcgaggt tagttgcgta agcagtttcg ctggttcatc acctgaaagc tgtgcgtcta
    11581 caggcaaata ccaccaattt tcttctgcaa aggcccggca tttcaccgca tctttttcag
    11641 tcattaccag cgtttgcccg gcgcttacca acgcactgac atccgcatgg ttcaaagact
    11701 gatgatcggc cagcggtaca catttttccg gttgtacgcc acacatcttc agcgtggcaa
    11761 aaaagcgcgg cggatgccca atccccgcca tcgccactac atgttcaagc tgagcaacgt
    11821 cacaacgcgt accggtacgt aaattcaccg cctgacccgg cagcagatgc atggggattt
    11881 caccgctgcg agggacaccg ccgttgacga ttaccgcatc aaccgacttt aagcgccccg
    11941 ctcgctcacg cattggcccc gccggcaacc accagccatt gccaaagcga cgcacaccat
    12001 caataacgac aatttccaca tcacgcgcca gacggtaatg ctgtaaaccg tcgtcggtta
    12061 cgatgatctg cacatcaggg tgttgcgcca gaatggcttt taccgcatca gaacgaacgg
    12121 gagaaaccgc aacaggcgca tcagtgcgtt gataaatcaa cacaggttca tcacccgcct
    12181 gtgctgttgt ggtatctgcc gacaataaca gcggataaga ttcagcctta ccaccatatc
    12241 cccgcgatac gacccccacg cgaataccgc gctgttgcaa ctgttccacc agccagacaa
    12301 cgaccggggt ttttccgttg ccgcctgcgg tgagattacc aaccacgaca accggtacgg
    12361 gggcacgcca ggcgcgcttc agttttagtt tatagcaaag acggatcgcg ccactcacca
    12421 ggccatacaa ccaggagagt ggcagcaata gccgccacaa aggggattca ccagaccaga
    12481 ttttttcgat cattggccaa actgcatttt gtgaagttgc gcgtaaacgc cgcggtgctc
    12541 aagcaaatcg ttatgcgtac cgcgttccac aatgacacca tcctcaacga ccacgatttc
    12601 gtctgccttt tcaatggtag acaagcggtg ggcaatcacc agagaggtac ggtttttctg
    12661 caactcatcc agtgccgcct gaatcgcacg ttcggattcg gtatccaacg ccgaggtagc
    12721 ttcgtccaga atcagaatcg ggctatcacg caacaaggct cgagcgatag caatacgctg
    12781 acgctgaccg ccagagagca gcacgccgtt ttcaccaatc actgtatcga gaccgttatc
    12841 catcttattg atgaagtcta tggcgtaggc catacgcgcc gcttcttcaa tttgctcacg
    12901 gctgtactgt tcagtccgtg cgtaagcaat gttgttagca accgtatcgt taaacagatg
    12961 gacattctgc gacaccagag caacctggtt acgtaacgac gccagggtat actcgcgcag
    13021 atcgtgacca tccatcagga tttcgccttc atcaatatcg taaaaacgcg tgatcaggct
    13081 ggcgatggtt gatttacctg aaccagagcg tccaaccaga gcgaccgttt tccctgccgg
    13141 aattttcagg ttgatgttac gcaatgcggg cacgtcacgt cccggataag taaaggtgac
    13201 attgcggaat tccacgtcgc cagtcgcacg ctcgatcacg cgcttacctt cgtctttctc
    13261 ctgctcactg tccagaatgg taaacagcgt ctgacaagcc gccataccgc gctggaactg
    13321 ggcgttaacg ttggtcagcg atttcagcgg acgcatcagt gcaatcattg aagagaaaac
    13381 aacggtaatc gtaccggcag tcaggttatc cataacactt gggaagctcg ccgcatacag
    13441 aacaaacgcc agcgccaaag aggcgattag ctgaatgatc ggatcagaga tggaagaggc
    13501 tgaaaccatt ttcatcccct gaagacgcat tcggttactg actttatcga agcgtttcgt
    13561 ttccacttcc tgaccaccga aaatcaatac ttctttgtgg cccttcagca tttgttctgc
    13621 gctggtggtc acctgcccca tggtgttctg catgttttta ctgatgttgc gaaaacgctt
    13681 cgatacaacg cgaatcgcaa tcgaaacaat cggtgccagc acaatcaaaa tgatcgacag
    13741 ttgccaactg taatagaaca tcatgatgaa caggccgatg attgacgcac cttcacgcac
    13801 aaccgttatc agtgcgccgg aagaagaaga agcaacctgt tcggaatcgt aggtgatacg
    13861 tgacaacagc gtacccgttg actgtttgtc aaaaaatgaa accggcattc ccatcatgtg
    13921 accaaacagg cggcgacgca tggtcattac cacctttcct gatacccagg agatacagta
    13981 gctggagaca tagctggtga taccacgtaa aatcatcagc ccgatcacca ccagcggcat
    14041 ccacaccagc acggagcgat ctgttttacc aaagccatca tcaagaagtg gcttaaggag
    14101 cgataacatg aaggtatcgc tggctgcgtt gaggattaac gctacgcccg ccacgatcag
    14161 acccgcttta aaaggcgcaa tggttggcca cagtcggcgg aacgtctgcc acgtagagag
    14221 atctttgtcg ttatgcattc aaaaaaccag catttgttga aatagccgca tattctaccc
    14281 gttatccact ggcacgccaa accactgatg ataccaacga ggtaaaattt gctccctgag
    14341 gctgctaatc cgccagcctt gcgctgaaaa attgaccgct atttgcccct gatgaggagt
    14401 atcaagccac ttataaccct gttgttgata gcgatgttta actttgcttg agggcaatcg
    14461 ccatgcgtta tagcgcgatg ccgatgcgag tgccactttt ccattcactc gctgaattaa
    14521 tggcaacgat gatgaggtat tactgccatg gtgaggtacc tgaagcaatg ttgtctgcac
    14581 ttgctgccag taacggctta gcatcttttg ttcagcgggg acttcaatat caccggttag
    14641 aagaatgcta ttcgtcccgt catcaacctt aaccacacag gaatggttat ttcctttatc
    14701 gttgctagct tgtaaaggcc agtgcacgct gaaacgcaat ccttgccatt gccacgcttc
    14761 gccacgcaca cagggctgat gatgttccca gtttaacgga cttctgatcc ataacatcgg
    14821 ccatgtgtgc aatattgagt ccagccctcc ccggtgatcc agatgttcat gactcagaat
    14881 aacgccttcc ggttcaagat tatgccagtg gagccagggg ataatcagtt gttgcccact
    14941 atccccttcg ggccaggcca gtcctgtgtc atagagaatc gctttgccgt ttctggctat
    15001 caccattgcc agcccttgcc cgacatcaag catgtacacc tgccactcgt caggtcgagg
    15061 tttttgccac agcggccagc acatcagcaa gcctacagcc acacacattg ctggcaacgt
    15121 tcgccaggcg tttaatcgcc ataccaccag taagaaccat ggggaaaatg atagccattg
    15181 ccaacgttca gcaatgttga tccacccttc tggcaaactc tttaaccccc agaaaagtaa
    15241 agccaaagac cggtcggcaa gaaaccataa cccttgctcc aggattaacg gcccgcttaa
    15301 atgcacaacc atcgcggcga ggatcaacgg aaccgtgata aatgtcacca agggaattgc
    15361 taatagattt gcaataaacg aggtcagact aatgccatga aatatgacga tttgcacggg
    15421 cataagcaga agtgtgatac ccagttgcag atggatgagg gaaacaactg cacgcaatac
    15481 cggcggcagt tgccactcag gacagggaaa ccactgatac caaaaaatca gtgccgcgac
    15541 cgcagcggca gagagccata aactttgcga gagaatggca acaggatcca tcagcaaaat
    15601 tgccgccaga caacatatcc atacatccca gccactccac tgtcgcccac ttaacttaag
    15661 cattccccac gtagcaagcg ccaccacggt acgcaatgca ggaggttgca ttcccgtcaa
    15721 ccaggcataa aaagcagcac agcagatccc accaattaat ggtatttgcc agtggatcca
    15781 gcgcccaggc agaaaaattt gtccactgcg aatgagtccg gcagccagca acaccgcaaa
    15841 agcgatgtgc aatcccgata tcgccattaa atgcgccgtt ccagtatcgc gcatgatatt
    15901 tttgatttct ttggggacgg ataaccgttc ccccatacct aaaccaagaa taaccgcatt
    15961 ccacggatag ggttgcagcg ttgtttgtag tgacgccaga tactgtgcac gcaggctaca
    16021 attcggttca ataagacttg cctgcagaaa gcggccggtg agcggctgat gctgggcaat
    16081 ggcataacgc tggctatcaa agccgccatc attaagttga ccatgaacgg cacgaacttt
    16141 gagtttcatt gaccacagtt gtccggcaca aaccgcttgc ggaagatatt cgccatacag
    16201 cacaaggcct ggcgcaggga atatacgtcg accttgtaga taagtaattt gaccgtaatg
    16261 agtggtcatg ccgtcagtag cagtgatctc aacaattgca tcctgcgtcg cgccagttaa
    16321 ggtttctcct gcccacaaaa tttgctttgc tgataatatg ccccaaacga aaaacagcaa
    16381 cgtcagcgcg atataacgga cggttttaac aggtataaat gccagtacgc aggcgaagag
    16441 agtcagaaac gcaagggcta atgtcccagg caattggggc aaaatcagca acggaaaaat
    16501 tccgcaaatt atgcatacgt cgactgtcgt tattttcatg cactcctccc tgtttatgga
    16561 aagtgtgcaa ctttgttgag cggttgtctg gtgggaattt tgcgttcccc gaggcacatt
    16621 cagaagaatt taacgagagg gtgaaaactg ctcaaaactc tgcgaggcag cttccagttc
    16681 ctgattagag aaatgaaaaa agcacccgac aggtgctttt ctcgcgttca agtttgagta
    16741 aaaacttaac cgtaaatatt ggcgcgatcg cgcagttctt taccaggttt aaagtgagga
    16801 acgtattttc cttccagttc tactttatcg ccagtcttcg gattacgtcc ggtacgtggt
    16861 gcgcggtagt gcaaagagaa actgccgaaa ccgcggattt caatacgctc gccctgcgca
    16921 agagtcgagg ccatatgctc cagcatctct tttactgcat cttcaaccgt cttggcggga
    16981 atgtgcgatt gctgggtggc aagtctttct atcaattctg acttggtcat gattcctccg
    17041 gttccttaaa ggcaaattaa gcggctgctg ttgctgcatt gattaagggc ggccgtagcc
    17101 gcccttagtg cttgattaca ggacgaaacc tgcaatctgt caagtaaact caacaaactt
    17161 cggaataaaa atcccgaaga gtcagagaat tactcgcctt tagctgcttt gaaagcttca
    17221 gccattgcgt tgttggagaa gtttgcatct tcctgtttgt taacagttgc gattgcatct
    17281 ttctcgtcag cttcgtcttt cgcacgaaca gacaggctga ttgcgcggtt tttacgatca
    17341 acgccggtga atttagcttc aacttcgtcg ccaacgctca gaaccaaggt agcgtcttca
    17401 acgcggtcac gagatgcttc agaagcacgc aggtaacctt caacgccgtc agccagttct
    17461 acggttgcgc ctttagcgtc aactgcagtt actttaccgg ttacgatagc gcctttcttg
    17521 ttcagagcaa cccagttgtt gaacggatct tctgcgagct gtttaacgcc cagggagata
    17581 cgttcacgtt ctgcgtcaac ctgcagaaca actgcagcga tttcgtcgcc ttttttgtat
    17641 tcacgaactg cttcttcgcc tgcaacgttc caggagatgt cagacaggtg aaccaggccg
    17701 tcgatgccgc cgtccaggcc gatgaagata ccgaagtcag tgatagactt gattttacct
    17761 tcaacacggt cgcccttgtt gtgggtttcc gcgaactgct gccacgggtt agctttgcac
    17821 tgtttcagac ccagggagat acgacgacgt tcttcgtcga tatccagaac cataacttcc
    17881 actacatcgc caacgttaac aactttggac gggtggatgt ttttgttggt ccaatccatt
    17941 tcggaaacgt gtaccagacc ttcaacgcct tcttcgattt caacgaagca gccgtagtca
    18001 gtcaggttgg tcacgcgacc agtcagtttg gtaccttccg gataacgttt agcgatagct
    18061 acccacggat cttcgcccag ctgtttcagg cccagggata cacgggtacg ttcgcggtcg
    18121 aacttcagca ctttaacagt gatttcgtcg cccacgttga cgatttcgct cggatgctta
    18181 acgcgtttcc aggccatgtc agtgatgtgc agcaggccgt caacgccgcc cagatcaacg
    18241 aatgcaccgt agtcagtgag gttcttaacg atacctttaa cttccatgcc ttcctgcagg
    18301 ttttccagca gctgatcgcg ctctgcgctg ttttcggatt cgataacggc acgacgagaa
    18361 acaacaacgt tgttgcgctt ctgatccagc ttgattactt taaattcaag ctctttgcct
    18421 tccaggtgca gagtgtcacg caccggacga acgtctacca gagaacctgg caggaacgca
    18481 cgaataccgt tcagctcaac agtgaagccg cccttaactt tgccgttgat aacaccggta
    18541 acagtttcag catcttcgta agctttttcc agcgtgatcc aggcttcgtg acgtttagct
    18601 ttctcacggg acagcagagt ttcaccgaag ccgtcttcta ctgcgtccag agcaacgtca
    18661 acttcgtcac ctacctggat ttccagctcg ccctgggcgt ttttgaactg ctcagccggg
    18721 atggcggact cagatttcag accagcgtca accagtacta cgtctttgtc gatagcaaca
    18781 acaacgccac gaacgataga acccgggcgg gtttcgattt cttttaagga ctcttcaaag
    18841 agttgagcaa aagattcagt catgtttaat cttcaggttt atatttaacg tccacctggc
    18901 tccatgccgg atggggttgt ttaacatacc cgctgtcatt ccattgcaac gggggtactg
    18961 caaattcggt cgcttatgcg agagccaatt tctggcgcgc gtattgtagc gctttttcaa
    19021 tcacttgctc aatgcttaag gtggtggaat ccaacactaa agcatcggat gccggaacca
    19081 gtggcgctac cgctcggtta cgatcgcggt cgtcgcgttc tttgatctcg gccaaaaggc
    19141 gctcaaagtt aacactaaag cccttctcct gcaactgtag catgcggcga tgcgcacgtt
    19201 cttccgagga ggcgtcaagg aaaattttca ctggcgcatc agggaatacc accgttccca
    19261 tgtcgcggcc atcggcaatc aggcctggta attcgcgaaa cgcgcgttgg cgacgcaata
    19321 atgcttcacg aacgcgtggg aatgccgcga cttgtgaagc tgcattcgcc acttcctgag
    19381 tacgaatttc gccgctgaca tcttcccctt cgaggatcac ttccagattg ccattggttg
    19441 acacaaaacg tacatccaga tgggatgcca gcggtaccag cgcatcttct gacgcaacat
    19501 caacatggtg atgtaatgcc gccaacgcca gtacgcgata aattgcaccc gaatccagca
    19561 gatgccattg caatgcttcc gccatagcct tacacaaggt gcctttccct gcaccgcttg
    19621 ggccatcaat ggtaataacc ggggcaattg ccgtcatctt tatctcctta aaacaggcgt
    19681 accgttaaca taaccgcgcg cattatacgc gccgacgcca caaatcgtta acgttgtgtg
    19741 caaaaaccag ttttgtctgt attcagatgg ggatttattg cgcaattata gcgggctggc
    19801 agaagaccag cccgaaagat gacaatgatt tacttaccag aggcgatacg atcacggatg
    19861 tgttgcgcac gctctgttga tggtgggtga gagtcaaaca tggactgggt gcgaccgcca
    19921 tccagactag ccagtttttc aaagctgcca accagcccct gggtgctgat gccacgtttc
    19981 ttcaacagat caaaggagaa atcatctgct tcggactctt tatcacggga gtatttagca
    20041 ttaatagcgc cttctgcgat atcacccaat tgtgagcggg aaagctggga agccacaccg
    20101 ctggtagctg aaatggcatc gcgtgcggca acgatcgcat aagaagcttt catttcagcc
    20161 agcgagtgac ccaacgcgac gtggcccagt tcatggccca gaacaccttc aatttcgttg
    20221 tcattcatca tgtccatcag gccactgtag acacggacac agccgttcgc catcgcccat
    20281 gcgttgacgt cgctggtcat ataaacctta tagttgaccg gcgtgccatt aatattgtta
    20341 cccaatgctt tggcgatttt tgccagacgt ttaccgtact tgctggattt acttgcgact
    20401 tgattgccgc tgtccatttc ggcacagccc tgattggcaa tcgcttttgc atcggcatcg
    20461 cttaatgttg ctgctttata ggcatttaaa ccggagctga tagccatatt ggtatcaata
    20521 ccgtgggtat tttgacaccc tgtcagtaat gctgcagagg tcgcaatcgc cagcagtaat
    20581 ttagtattct tcatgtgaat catccgttga ttaatcatat ataaatatta tgtatcaaca
    20641 acttacattg acaagcgacg ctaccattaa tcaaaatgac tcacaaggtc cgaaaaaaaa
    20701 cgccgacaat g
//
LOCUS       DAJTZL010000068        20441 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.068, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000068 DAJTZL010000000
VERSION     DAJTZL010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20441
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.068"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..1132
                     /gene="ypdA"
                     /locus_tag="OO544_002105"
     CDS             <1..1132
                     /gene="ypdA"
                     /locus_tag="OO544_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HCR6767703.1"
                     /translation="LVQSVEGEKEASAARQTKLALDIANKTLPLFRHVNSESLRKVCE
                     IIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQAMNYGKIIIKNNDEA
                     HRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAVGLSQIISTQLEVSRA
                     EQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTARQLIFNLSRYLRYNIE
                     LKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNCCIPSLLIQPLVENAI
                     VHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERVEANEMPGNKIGLLNV
                     HHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
     gene            1147..1881
                     /gene="ypdB"
                     /locus_tag="OO544_002106"
     CDS             1147..1881
                     /gene="ypdB"
                     /locus_tag="OO544_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HCR6767704.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            1894..2751
                     /locus_tag="OO544_002107"
     CDS             1894..2751
                     /locus_tag="OO544_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6767705.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(2754..5249)
                     /gene="ptsP"
                     /locus_tag="OO544_002108"
                     /pseudo
     CDS             complement(2754..5249)
                     /gene="ptsP"
                     /locus_tag="OO544_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
     gene            complement(5274..6311)
                     /gene="ypdE"
                     /locus_tag="OO544_002109"
     CDS             complement(5274..6311)
                     /gene="ypdE"
                     /locus_tag="OO544_002109"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCR6767706.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARSYDEVMQAGIRPGDRVTFDTAFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVSAEIGVPLQAD
                     MFSNGGTDGGAVHLTDTGVPTVVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(6311..7396)
                     /gene="ypdF"
                     /locus_tag="OO544_002110"
                     /pseudo
     CDS             complement(6311..7396)
                     /gene="ypdF"
                     /locus_tag="OO544_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            complement(7411..8658)
                     /locus_tag="OO544_002111"
     CDS             complement(7411..8658)
                     /locus_tag="OO544_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HCR6767707.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(8680..9006)
                     /locus_tag="OO544_002112"
     CDS             complement(8680..9006)
                     /locus_tag="OO544_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-like phosphotransferase enzyme IIB
                     component 1"
                     /protein_id="HCR6767708.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(9226..10191)
                     /gene="glk"
                     /locus_tag="OO544_002113"
     CDS             complement(9226..10191)
                     /gene="glk"
                     /locus_tag="OO544_002113"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HCR6767709.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALTLGTFGGVYIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            10395..11651
                     /locus_tag="OO544_002114"
     CDS             10395..11651
                     /locus_tag="OO544_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HCR6767710.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPIWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            11766..12092
                     /locus_tag="OO544_002115"
     CDS             11766..12092
                     /locus_tag="OO544_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HCR6767711.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(12233..13471)
                     /locus_tag="OO544_002116"
     CDS             complement(12233..13471)
                     /locus_tag="OO544_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HCR6767712.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            13807..15009
                     /gene="nupC"
                     /locus_tag="OO544_002117"
     CDS             13807..15009
                     /gene="nupC"
                     /locus_tag="OO544_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HCR6767713.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLRFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(15059..17247)
                     /locus_tag="OO544_002118"
                     /pseudo
     CDS             complement(15059..17247)
                     /locus_tag="OO544_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311300.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
     gene            complement(17456..17531)
                     /locus_tag="OO544_002119"
     tRNA            complement(17456..17531)
                     /locus_tag="OO544_002119"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17496..17498),aa:Ala,seq:ggc)
     gene            17752..18111
                     /locus_tag="OO544_002120"
     CDS             17752..18111
                     /locus_tag="OO544_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HCR6767714.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            18113..18505
                     /locus_tag="OO544_002121"
     CDS             18113..18505
                     /locus_tag="OO544_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HCR6767715.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQGKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(18557..19972)
                     /gene="gltX"
                     /locus_tag="OO544_002122"
     CDS             complement(18557..19972)
                     /gene="gltX"
                     /locus_tag="OO544_002122"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HCR6767716.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            20231..20306
                     /locus_tag="OO544_002123"
     tRNA            20231..20306
                     /locus_tag="OO544_002123"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20264..20266,aa:Val,seq:tac)
     gene            20352..20427
                     /locus_tag="OO544_002124"
     tRNA            20352..20427
                     /locus_tag="OO544_002124"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20385..20387,aa:Val,seq:tac)
BASE COUNT         5079 a         5455 c         5081 g         4826 t
ORIGIN      
        1 tctggtgcaa agcgttgagg gcgaaaaaga ggccagcgcc gcgcggcaga ccaagctggc
       61 gctggatatc gccaacaaaa cgctaccgct gtttcgccat gtcaatagcg agtcattacg
      121 caaggtctgc gaaattatcc gcgatgacat tcacgccgat gcggtggcga ttactaatac
      181 cgatcatgtg ctggcctatg ttggcgtggg tgaacataac tatcagaatg gcgatgactt
      241 cattagcccg actacccgtc aggcgatgaa ttacggaaaa atcatcatta aaaacaatga
      301 tgaagcccac cgcacaccag agattcattc catgctggtg atcccattgt gggagaaagg
      361 ggtcgtgacc ggaacgctga aaatttacta ctgccacgcg catcagatca cctcgtcatt
      421 acaggaaatg gcggtcggtc tgtcgcaaat catctccacg caactggagg tttcacgcgc
      481 cgagcagcta cgtgaaatgg caaataaggc agagcttcgc gccctgcaaa gcaaaattaa
      541 tccccatttt ctgtttaacg ctctgaacgc tatttcatcg tcaatccgtc tgaatccgga
      601 taccgctcgc cagttgatct ttaatctgtc gcgttatctg cgctataaca ttgaattaaa
      661 agacgatgag caaatcgata tcaaaaaaga gctgtatcaa attaaagact atattgccat
      721 tgagcaggcc cgctttggtg acaagctgac ggttatctat gatattgatg aagaggtgaa
      781 ttgctgcatt cccagcctgc tgatccagcc gttggtggag aacgccattg tccacggtat
      841 tcagccttgc aaaggtaaag gcgttgtcac catcagcgtt gcagagtgcg gaaatcgggt
      901 acgcattgcg gtgcgagata ccgggcacgg catcgatcca aaggtgattg agcgggtcga
      961 agcgaatgaa atgccgggca ataaaattgg cctgctgaat gtccatcatc gcgtgaagtt
     1021 attgtatggc gaggggctgc atatccgccg cctggagccg gggacggaaa ttgcgtttta
     1081 cattcctaac caacgcaccc cagtcgcctc acaggctacg ttattgcttt gagccggagt
     1141 gatattgtga aagtcatcat tgttgaagac gaattcctgg cacaacagga actgagctgg
     1201 ctaattaaag agcacagcca gatggagatt gtcggcacct ttgacgacgg tctggacgtg
     1261 ttgaagtttt tgcagcataa ccgcgtcgac gccatttttc tggatatcaa tattccgtcg
     1321 ctggatggcg tgttgctggc gcaaaacatc agccagttcg cccataaacc gtttattgtg
     1381 ttcatcaccg cgtggaaaga acatgcggta gaagcgtttg aactggaggc gtttgactac
     1441 attctcaaac cgtatcagga gtcacgtatt accgggatgc tgcaaaaact ggaagcggcc
     1501 tggcaacaac agcagaccag cagtacgcct gccgcgacgg taacgcgtga gaatgacacc
     1561 attaatctgg tgaaagatga gcgaataatc gtcacgccaa ttaacgatat ctattacgcc
     1621 gaagcgcacg agaaaatgac ctttgtctat acgcggcgtg aatcctacgt aatgccgatg
     1681 aacattaccg aattttgcag caaactgccg ccgtcgcatt ttttccgctg ccatcgctca
     1741 ttttgtgtca atctgaacaa aatacgcgaa atcgaaccgt ggtttaataa cacctacatt
     1801 ctgcgactga aagatctgga ttttgaagtg ccggtcagcc gcagcaaagt gaaagaattt
     1861 cgccagttaa tgcatcttta atcgggaact ttcatgaaag caccaggctt gcccgccgat
     1921 caacaatttt tcgccgatct gttcagcggc ctggtgctta acccgcaact actggggcgc
     1981 gtctggtttg ccagccagcc tgcctcgtta ccggtgggca gtttatgtat tgattttccc
     2041 cgtctggata tcgtgctgcg cggcgaatac ggcaacctgc tggaagcaaa gcagcagcga
     2101 atggtggaag gagaaatgct gtttattccg gcacgcgcgg ctaatttacc gatcaacaac
     2161 aaaccggtga tgctgttaag cctggtgttc gcaccgacct ggcttgggtt atcgttttac
     2221 gatagccgca ccacgtcgtt gttgcatcct gctcgccaga tccagcttcc cagcctgcaa
     2281 cgcggtgaag gtgaagcgat gcttaccgcc ctcacccatc ttagccgttc gccgctggag
     2341 cagaatatca ttcagccact ggtgttaagt ttgctgcatc tttgccgcaa cgtggtgaat
     2401 atgccgccgg gtaattcgca accgcgcggc gattttctct atcacagcat ttgtaactgg
     2461 gttcaggata attatgccca gccgctcacc cgcgagagcg tggcgcagtt ttttaatatc
     2521 acgcccaatc atctgtcaaa actgtttgct cagcatggaa cgatgcgttt tatcgagtat
     2581 gtgcgttggg tgcgaatggc gaaggcgagg atgattttgc agaaatatca tctgtcaatt
     2641 catgaagtgg cacagcgttg cggttttccg gatagcgact atttttgtcg cgttttccgg
     2701 cgtcagtttg gtctgacgcc gggagagtac agcgcccgtt ttcagggcta acgtcagaag
     2761 gttaattctg tttccagcag cgtcaggata ctttgtgcat cttgcgcggc aaacaacgac
     2821 tggcggaagt ttttattcac cagtttacgc gccagctgcg agaagacttt cacatgattc
     2881 atcccttcgt tagcacccag cgtcagcatg atcaccagtt caacttcgcc catttctgac
     2941 tgccagtcaa ccggtttcgc cagccgggca atgctgatac tggaatgacg gatccactga
     3001 gatttggtgt gcgggatcgc tacgccaaaa ccaacgccgg tggtaacaat ctcttcccgc
     3061 tgccagacat cttcttccag ctcaaacgga tgttcagtgc gcccgttaac gccgaggttg
     3121 ccgcacagga actggatcgc ctgctcttta ttgctaaaat cctgatcaac aaagatattc
     3181 tccagcgcca gcagtgggcg aacgtcttct tccggcgtaa aggcggtgag taacgcttca
     3241 atttcctgcg cactgcggca ttcacatgcc tgacgcgcca gttcccgaca cgcctcgcta
     3301 tccagttgac gaagctggct tttcaccgcc ggaatacgcg ggctactcat actcagctca
     3361 tccaggccca gcccaagcag tagcggcaga taacggcttt caccgcccag ttcaccgcaa
     3421 atgcctaccc atttgccccg ctgatgcgca gtggtaacta tttgctgcaa catgcgcagg
     3481 aacgatggcg taatcgggtt atatagcggc gatacgcgcg ggttattacg atcgaccgca
     3541 tacagatact gggtcatatc gttggagccg atactgaaga aatcgacctc atcgcagaag
     3601 tggtcgatga tgtagcacac cgacggcact tccaccatga tcccaagcgt aatcgtctct
     3661 gcatgacgca ggccatcgcg cttaagctca acgatcgctt tttggatctc gcctttcacc
     3721 cataagatct gatcgaggct gttaaccatc gggatcatca actgggcgtt gccgaaactg
     3781 gcggcgcgca aaatggcccg cagttgagtg cggaacaggc cagcaaattc cgggtaaata
     3841 cgtaccgcgc gatagccgag gaacgggttc tcttcctggg gaatattcag ataaggaatg
     3901 cttttatcgc cgccgatgtc catcgtgcgg aagataatcg gcttgtcgcc cgccgccagt
     3961 agcacctgct ggtaggcttc aaattgctcc tgctcgtccg gcgcgctgtc gcgatccata
     4021 tagagcattt cggtacggaa cagcccgaca ccttccgcgc cgttggcaaa cgcgcctggc
     4081 gcttccagag cggtgccgat attcgccgca atatcaatac gcttgttatc acgggaatag
     4141 gccagctgcg cggcagcctg cgcctgttgt ttttggcgtt tatccgccag cgtctgcgcg
     4201 acctgataat aaccgctcac cgcgtcattc gggttaatcg ccagcacgcc gcactgggcg
     4261 tcaagcactg caggttgccc ggcataacgg gcaatcgcat ccagcggcaa gccactcagt
     4321 accgggatcg ccgaggcacg ggccagaatc agtgtatgcg aggtgcgccc ggttttctcc
     4381 agaatcatgc ccgcaagatt tttcaaatca aggctcaaaa actggcttgg ggttaaatct
     4441 tcagccacca gaatggtcgg tttttccagc accagattgt tgcgcggctt cagttccggc
     4501 taggtgatat gcagcaactg ttcgctgata tcgcgaatgt cgctgacccg ctcgcgcaga
     4561 taatcgctgg cagaagcaga tagtttggcg caaacctgct ccatattgct gatgatcgcc
     4621 gcccccagtc cctgatgctg ttctgtcatc aggcgacgga tattgcctgc aaattcatca
     4681 tcctgaatca gcgacaaatg agcgctgagg atagttttgc tttcgccgtc acgctcacgc
     4741 aattgctggt tcagttgctc ggcaagcgtt gccaggctgt gctccagccg ggtggaatct
     4801 tgcgcactgg cggggattgc ccgataactg tcgaggctgt cgctttgtaa cagggtcagc
     4861 gtacccacgc cgacgccgct tgccagcaca ttgccgtaca gtaaatccgg gttaaggcgg
     4921 cttaatgaac gcggcagcgg atgcgccgtc agttctgcct gcgtcggctg aacgctgtcg
     4981 ctgtcgataa agcgcaccag gatgtactct tccagcacgc gccgcgcctg ctcttcatcg
     5041 ctgccgctaa tgttcaggct gcaactgtca ttaaataagg tgccagttcc aatcaacgcc
     5101 agcgagcttt tagcatcggc ctttgcgttc tggcgatggt tgataaaggt gatttcgctt
     5161 tgccactggc tgcactgttc tttaagttcc cacgccggac gagcatgtag accgttaggc
     5221 aaaggacaga gaaattgaat cgttaacata accgctccgg gttaatcagg agatcatctg
     5281 aaatccgtca gttgaacaac cgtctcacgc gtaagacgtt gaataagggc agataaaagt
     5341 tgctccatct gcaaaatgtc gcggcaatcg gcaatcgatg ccgcgcaatg tccgtggcgg
     5401 gtggcaggcc ccattaccac tgtgggtacg ccagtatcgg ttaagtgcac cgctccaccg
     5461 tccgtgccgc cgttactgaa catatccgcc tgcaacggaa cgccaatttc tgccgacacg
     5521 gtttcaatcc acgcggtaag ttttggcggc gcaatcagcg atttatcgct taacaccagc
     5581 atcggcccgt taccaatctg acgatggtta gccgcgccat aatcaaagtt tttcgcccag
     5641 caggcggtat caagcacaat ggcgacgtcc ggcgacaccg cgcgggtagc agtttgcccg
     5701 ccgcgtaatc ccacctcttc gctggaactt gccaccagcc acacttccgc aggcagttcg
     5761 gcgtcgtgca gctcacgtag taacgtcacc agcagatagc aaccgaggcg gtcatcaaag
     5821 gctttcccca tcacccgctg gtgagggaga acctgaaaag ctgtatcaaa ggtgacgcga
     5881 tcgcctggac gaattcccgc ctgcatcact tcgtcatacg agcgcgcacc aatgtccacg
     5941 cgcatggcgc tgacgtcatt cccctgccgg tcgccgtcaa gcaggcctgg aattttgcac
     6001 tcttcacggg tggtgatgcg caccggctgc agctggcggg cagccatgcg cacgttgcca
     6061 accggcagca catcaattgc cccttcgcgg gagatgctgc gcaccataaa tcccacttcg
     6121 tccatatgcg cacagatcat caccttcgga cctgtcgatt cattgaggcg gatcagcacc
     6181 gatcccagac catcaaatcg tacttctttt tgcaggcgat ccgcttcttc cagtaggatc
     6241 tgccgcactt cctgttccga ggaggcgatc gcatctgcct cgctcaacgc ttttaatagc
     6301 gataaatcca ttatgcctct cccgtgagca acactgtttt cggcatggcg tagagcactt
     6361 ctgcgccttg cggggtaacc agcacaacat cttcgatgcg cacgccccct tgccctggca
     6421 aataaatccc cggctccacg gtcagtaaca tgcctggctg tagcgtcgtg gtatcccgcg
     6481 gtgaaaagcg tggatcttcg tgtacttcaa taccgatagc atgaccggtg ttgtgaccaa
     6541 aatagtcgcc aaatcctgcc tcggtaatca cccggcgcgc ggcgtcgtca acctgttagc
     6601 agcgtacgcc agggcgaatc gcggagattg ccgccagctg tgcttgcagg acaatctgat
     6661 agacgttaaa cagcgggtga gattcggcaa ttaccccttc gccattcacc agcaaggtgc
     6721 gcgtcatatc agagcagtac ccctgataga gcgcaccaaa atcaagagtg acaaactcgc
     6781 ctgctgcaac aatcttgtcg ctggctttgc cgtgcggcag cgccccacgc cagccgctgg
     6841 cgacaatggt gtcaaaagag gctttttctg cgccctgctg gcgcataaac cactccagtt
     6901 cagcggctat ctcgcgctcg ctcatccccg cctgaataaa gcggcgaata tgctccgctc
     6961 cgcgatcggc aatcccacag gcgaggcgga ttatctccac ctcctctggc gttttgattt
     7021 gccgcagcac atccggcgtg gcgctaacca gtttcgcatt gagttcagac ttccagcggt
     7081 gcgcggtttc ccaactcacc tgctggcctt caaaaccgag cgtttgcaac tgctcatcgg
     7141 caatgatttg attgacgata gtggtaagcg tgttcgtcgc gtcaagcaaa tgcagctggt
     7201 agccttgcgc acgggcttcc acctcaacga aatagcgcga atctaccaga atgtgcgcac
     7261 tttcacggct aataaccaca tagcctgatc cggtggagat ccccagatgc ggctgtttgt
     7321 tctgccgtga ggagagaagc actgcatcca gttgttgcgc cttaagccag tcgcgcagcg
     7381 aagcgagtaa tgtcatctgg attttccttc ttacaggcta tcgattaaca atttaccttt
     7441 acggaacatc atcagacgca ggaacaccac catcagcgca gttatgactg cccccagcgc
     7501 gatccccgcc atatagacgc ccaggttagt gaccagcggc catgcccaga tagcggattc
     7561 cgggaaccat tgcactgcgc ccagccagac agcggcggtt gagccgacaa tcgcgccgac
     7621 catatacgac ggaatggcgg tgatggggct ttccagcgca aacggaatcg caccctcact
     7681 aatccccata aaggcaagga acatcgcggt cttaccctgc ggataaagtt gagcgttgaa
     7741 caggcgtttg ccggttaaac gacggtcgat aatggtcgcc agacccagac ctatcggcgg
     7801 aataacgata gcgatagaac gtgcagtgac cggtaatacg tggtcagtgg taaagctgaa
     7861 ggcaacaaaa cctgcggctt tgttgatcgg gccaccaagg tcgattgcgg tcgccgcggc
     7921 gatccccatt gcgtacatca gcgcgccctt ctcacctgcg gcagtcagca cggtacggat
     7981 accgccgttg atccagccgc caaagggggt aatgacgtag tacatcgcca gcatgacaaa
     8041 aatggcggaa agaatcggta gcaaaaatgt ggttttgaac gctaacagga aatccggtaa
     8101 ctggattttc tggttcatcc acttcacaag gtaacccgcg acgatagaaa taatcagcgc
     8161 accaatgaag gtggacggca ccggcgaaga ggtcgcccat tgcatagtgc tgggatcgaa
     8221 gttcagcagt tgcgtcggct gggtagacat caagccgccg ataaaaccag ccgggaacgc
     8281 cagtttgcca ccgatagagt tcgccacaaa agcagcaaac atcggaatgg caaaaccaaa
     8341 caacacgccg ccgaaggact gcgacagcca ggcaaatttc agtaaggaga ggtcgaaacc
     8401 ggagaatttc ccgctattaa gagcatccat gatgccgatc tcagcaggaa ttttaagcca
     8461 gctataagca atcagctggc tgaaagcgag gatcacaccg cccataatta aggtcggcac
     8521 catgcgtgaa atccctgaca tcacatgctg cgggagttca ccccagaagc tgcttttaga
     8581 ggcctgcgga ttcttaactg ccgctgccgc accggatgcg ccaggcacaa cggttgcact
     8641 gcgtttttta atggccataa caattatccc tgtcgaatat tactgttgtt cagaggcaat
     8701 catctcttcg atttctttga tgatgcccgc agcgttttta attgcgtcct gcaaagtgat
     8761 ttcataaacg tcgcgtgatt cgaaacgctc attatcttcc ggggtaactg ccacggagtg
     8821 gatgatgatg gttgcttcgg cgatatcctg cgccgtcagg cgattctgga taccgtccgc
     8881 gccctgggtt tcaattttca cttcataacc tgcttctacc gccgcttctt ccagcgcctg
     8941 agcggccata aaggtgtgag ccaggcccat tgggcaggca cataaggcaa ttagtttctt
     9001 actcatcgtt aattcctcgt tcagtgatag ttgacgagtg aaattatgaa cgtgcaaaac
     9061 aaatcgccgt taccagacaa ataatgcatt tactggagaa ttaatcccgc tgtacggaag
     9121 tgtgacgcag gtcggccttg tgagggggac acaaagaaaa attgaattaa ctgtatgatt
     9181 taaaagatta tcgggagagt tacctcccga tataacagaa aggatttaca gaatgtgccc
     9241 taaggtctgg cgtaaatgtg caccggaacc gagaagaccc ggattgtcat ggacgatgag
     9301 atacaccgga atatcatgga catattcttt aaagcgccct ttatcttcaa atgcggcacg
     9361 gaaaccagag gctttgaaga actcaaggaa gcgcggcacg ataccgccag caatataaac
     9421 cccaccaaag gttcccagcg ttaatgccag attgccgcca aaacggccca taatgacgca
     9481 aaacagcgac aacgcacggc ggcaatcggt gcagctgtca gccagcgcgc gttcggtaat
     9541 atcttttggc ttgagatttt ctggcaggcg gttgtcggct ttaacaattg cgcgatacaa
     9601 attcaccagc ccaggaccag aaagcacgcg ttccgccgaa acatgaccaa tttccgcacg
     9661 caatatttcg aggataatgg cctcttcttc actattcggc gcaaaatcaa cgtgaccgcc
     9721 ttcgcccggc aagcttaccc aacgcttatc gacatggacc agatgcgcaa ccccaagccc
     9781 tgttccggca ccgtaaaccg caataggttt accttcgacc ggttctgcgc caccaaactg
     9841 aatcagatgc tcttttttca gcatcgggat cgccatcgat acagcggtaa aatcgttaat
     9901 aatttccaga tggctaaaac cgagattctt tttcatttcg gcaattgaga acgcccaggt
     9961 atggttggtc attgccaccc agtcaccggt aattgggcaa gcgatggcaa tacagccgtc
    10021 tttcacctcg accttatgtt cttcaagata aacgcgaatg accgcttcga ggctggggta
    10081 atcaagccct gaataggtct tagcctgcga gatttcacca ctggcaatat cacacagagc
    10141 aagacgtgcg ttggtgccgc ccacatcacc gactaatgca tactttgtca ttcttcaact
    10201 gctccgctaa agtcaaaata attctttctc acactgtaaa tacctggggg cataacaaca
    10261 acgccggaaa ggcaggctcc ctgtaaatat cgatctgggt cacacattta ctttatcgtt
    10321 tcagcaccaa ttgcagcgat gcctttttgc aagctgggca aactaagtat ctgaccccgc
    10381 ataaggaata gaacatgctc catccgcgag ccagaaccat gttgttatta tcgctccccg
    10441 ccgtggcaat tgggattgcg tccagtctta ttctgattgt ggtgatgaaa atcgcctcgg
    10501 tattacagaa tttgctctgg caacgactgc cgggaactct ggggatagcc caggattcac
    10561 ctatctggat catcggtgta ttaacgctaa cgggtattgc ggtggggttg gttatccgtt
    10621 tcagccaggg tcatgccgga ccagaccccg cctgtgaacc gctgatcggc gcaccagttc
    10681 cgccctctgc gctaccagga cttatcgtag cattaattct cggtcttgct ggcggcgtca
    10741 gcctggggcc ggaacatccg atcatgaccg tcaatatcgc ccttgccgtc gccattggcg
    10801 ctcgtctgtt accgcgcgtc aaccgaatgg agtggactat tttagcctct gccggaacca
    10861 tcggcgcgct gtttggtaca cctgttgcgg cggcgttgat attttcgcaa accttaaatg
    10921 gcagtagtga agttccgcta tgggatcgtc tttttgcgcc gttaatggcg gcagcagctg
    10981 gtgcacttac taccggatta tttttccatc cccatttttc actgcccatt gcgcattacg
    11041 gacagatgga aatgaccgat attctcagcg gtgcaattgt cgcggcgatt gccatcgcag
    11101 cagggatggt cgccgtatgg tgcttaccac ggttgcacgc gatgatgaat caaatgaaaa
    11161 atccggtgct cgtgctgggt attggcggat ttattctcgg aattctgggg gttattggtg
    11221 gaccagtttc gctgtttaaa gggctggatg agatgcagca gatggtggca aatcaggctt
    11281 tcagcaccag cgattacttt ttgctggcgg taattaaact tgccgccctg gtcgttgctg
    11341 ccgccagtgg ctttcgcggt gggcgaatct tcccggcagt gtttgtcggc gtggcattag
    11401 ggttgatgct gcatgagcac gttcccgccg ttccagcggc aataaccgtt tcctgcgcta
    11461 ttctcggcat cgtgctggtg gtaacacgcg atggctggtt aagtcttttt atggcggcag
    11521 tcgttgtacc caataccaca ttgctaccgc tgctctgtat cgtcatgctt ccggcatggc
    11581 tgttattagc aggtaagccg atgatgatgg tcaatcgtcc gaagcaacag ccaccccacg
    11641 ataatgttta gcaaaaaatg ctctccttta tgatttaaga gttatggcga tgatacgggg
    11701 ctttacctcc ccgtaatatt gcgttaacag gccgctgacg gcttcgatcg tgaaggagaa
    11761 taacaatgtt caggtcactg tttctggcgg ccgccctgat ggcatttacc ccgcttgcag
    11821 caaatgcagg tgaaatcacc ctactgccat cgatcaaatt acaaattggc gatcgcgatc
    11881 attacggtaa ttactgggac ggtggtcact ggcgagatcg tgattactgg catcgcaatt
    11941 atgaatggcg caaaaaccgc tggtggcgtc atgataatgg ctatcatcgt ggctgggata
    12001 agcgtaaagc atatgagcgt ggctatcgtg aaggctggcg cgatcgtgac gatcatcgcg
    12061 gaaaaggccg cggacatggg caccgccatt aaacgcgtcc tccaatggag cacaatgcct
    12121 gatgcgacgc tagcgcgtct tatcaggcct acaaaccggc tctttaacgc gtagggcgga
    12181 taaggcgttt acgccgcatc cgccaatagt gccagatgcg acgctcattc aactacaatc
    12241 ccagcgccgt ccccaccaac aaccagatat tcagcgccac gaccagcact acaatcaccc
    12301 agcctgtctg ttttacgcgt ttgctgttca ccagatcgcc catcaacttg ctgtcactgg
    12361 tgaaaatcag cagtggaacc agcgccaggg cgataccaaa acttaacagc acctgactca
    12421 taaccagaat ccgtgtcgga tctaatccca tcagaatgac aataaatgac ggcaacatgg
    12481 tgactgtacg acgcacccac agcgggatat ggaagcgaat gaatccctgc atcaccacct
    12541 gccccgccag tgtccccacc accgttgagg acagtccggc agcaaccaga cttaacccaa
    12601 agaccgttgc cgcagcatgg cttaacagcg gttgcagcgt cagataagcc tcatcaagat
    12661 cggcaacacc agtatgacca gaaaagtgga acgccgccgc agctgtagcc atcatcgcca
    12721 gattgacaaa accggcaatc gtcatggcga tagccacatc ccatttggtg gcggaataac
    12781 gttgttgacg cgaaccgcca tgtaaatgct gagtgagcga agagtgcaaa taaatcacat
    12841 gcggcataat cgtcgcccct aacacgcctg ctgccaggaa gaccgcctcc gaagtaggta
    12901 aactcgggat caccattcct ttacccagct gcgccaggtt aggctgggag aaaatcaact
    12961 cgacaatgta agccgcggca acaaacaaca gtaacccgcc aatcactttc tccagtggtt
    13021 tttgcccgcg acgttgcagc attaaaatca ggaaagtcgc gatccccgtc agcaccgcgc
    13081 cctgcaacaa cgagacacca agaatgagtt taaaaccgat cgccgcacca ataaattccg
    13141 ccaggtcggt tgccatcgca ataatttctg cctgaaccca atagaaccac actacgggac
    13201 gcggatagtg atcacgaatc tgctccgcca gattttttcc ggtggcaatc cctagtttgg
    13261 cagagaggat ctgaatcagc atcgccatca ggttggccca aacgacaacc cacagtagct
    13321 gatagccgaa gctggcaccc gcctgaatat tggtcgcaaa gttaccggga tcgatataac
    13381 caatcgccgc aatgaacgca ggtcccatta atgcgagcct catcttgcgc gccgcccgtc
    13441 cgctgctact ctcaacgcga tagttcgtca tcttgtgcct ctaaaacata gcctttgcta
    13501 tgtttcatgc tatgataaac gagaatgatt atcaaattca tttaaatgga ttgtggtgat
    13561 ttctctgata gaccaggatt atgactacga aaagattgat gacttgaatg ttatggttat
    13621 gtttaatgtt agcacattta cataactttc agcttccata cacaacatag cagaaatgta
    13681 tgacagatca ctatttttga agcctgtcac aggacgtcat tatagtgtgt gtcagatctc
    13741 gttttcctta accatgttac atagaatgtg cacggaaatt taacctgcct catatttgga
    13801 gcaaatatgg accgcgtcct tcattttgta ctggcacttg ccgttgttgc gattctcgca
    13861 ctgctggtaa gcagcgaccg caaaaaaatt cgtatccgtt atgttattca actgcttgtt
    13921 atcgaagtgt tactggcgtg gttcttcctg aactccgacg ttggtttagg cttcgtgaaa
    13981 ggcttctccg aaatgttcga aaaactgctc agatttgcca acgaagggac taacttcgtc
    14041 tttggtagca tgaatgatca aggcctggca ttcttcttcc tgaaagtgct gtgcccaatc
    14101 gtctttatct ctgcactgat cggtattctc cagcacattc gcgtgttgcc ggtgattatc
    14161 cgcgcaattg gtttcctgct ctccaaagtc aacggtatgg gcaaactgga atcctttaac
    14221 gccgtcagct ccctgattct gggtcagtct gaaaacttta ttgcctataa agatatcctc
    14281 ggcaaaatct cccgtaatcg tatgtacacc atggctgcca cggcaatgtc caccgtgtcg
    14341 atgtccatcg ttggtgcata catgaccatg ctggaaccga aatacgtcgt tgctgcgctg
    14401 gtactgaaca tgttcagcac ctttatcgtg ctgtcgctga tcaatcctta ccgtgttgat
    14461 gccagtgaag aaaacatcca gatgtccaac ctgcacgaag gtcagagctt cttcgaaatg
    14521 ctgggtgaat acattctggc aggtttcaaa gttgccatta tcgttgccgc gatgctgatc
    14581 ggctttatcg ccctgatcgc cgcgctgaac gcactgtttg ccaccgttac tggctggttt
    14641 ggctacagca tctccttcca gggcatcctg ggctacatct tctatccgat tgcatgggtg
    14701 atgggtgttc cttccagtga agcactgcaa gtgggcagta tcatggcgac caaactggtt
    14761 tccaacgagt tcgttgcgat gatggatctg cagaaaattg cttccacgct ctctccgcgt
    14821 gctgaaggca tcatctctgt gttcctggtt tccttcgcta acttctcttc aatcgggatt
    14881 atcgcaggtg cagttaaagg cctgaatgaa gagcaaggta acgtggtttc tcgcttcggt
    14941 ctgaaactgg tttacggctc taccctggtg agtgtgctgt ctgcgtcaat cgcagcactg
    15001 gtgctgtaaa accatacata aaaaagccgg ggatcattcc ccggcttttt tacgcctgtt
    15061 aatcagctaa tggctgcggg cgaccaatca aatacccttg cagatattgc accccgagct
    15121 tatgcaatag cgcctgctgc tgttgcgtct cgacaaactc cgcgaccaca ctcaatgact
    15181 tcgctttcgc cagatcggta attgatctca caatcatcgc atccagcgta ttcgtgacaa
    15241 tatctttcac aaagacgcca tcaattttga tgatatcagc ctgcaaacgc tttaaccgtt
    15301 cgtagttggc atatccggtg ccaaagtcat caatcgcaat ccggaaacca aacttatgca
    15361 gctgctcgat gttgtacatg ctgctttctg cgttagaaaa cgcctgctcc tcggtgatct
    15421 caagaatgac cgcctgcggg gagatgtgat aacgcttaaa cagacgaata atccgcccgg
    15481 caatattctt ttgcagcagc gtgagcggca ttaaattgac tgaaaagcgc ggtccttttt
    15541 tgtcgcaagg gtgtgttgcc agccacttca acaaggattc cagcacttgc aaatcaaaac
    15601 gcgcgctaag gttaaactga gcaataaggg gcagaaactt atccggggtc ataatgccgc
    15661 cgtcatattt cagtcgcgcg aggatctcat cataaccttc accctctttg ttgcgaattg
    15721 gctgggcgta gagcagcaaa tcaccctgat ctaacgccgt gcgaatggta ttcagcaata
    15781 gcacctgttt agtggtctgc ccggaaacca tctcctctct gctatccagc gccagcacat
    15841 gatgatgtgc gcaggattgc tccgccagcc agcttaactg ccccaacaag ggttgcaggg
    15901 tttcctgatt tccatcaaaa cgcccccagg cagcaccata gcccatatcc agcccggtat
    15961 tgttccagtg aatttgccga ctattcagga tgttaaccat atgctggagt cgcccttccg
    16021 tttccggccc gctcagcact aacagcagtt cactacccgg caattgatat aacttttcgt
    16081 tttcctgcat caacggcagc agcgtacggt aaattgatcg gatacagtga acgcgcatca
    16141 ttaagccgta atgacgactc ataaactcaa gattatcaat gcgcaggcag caaaactctt
    16201 gcccgcctct tgctccggcg cttgttccaa cgcacggaag ttgggcagga gcgttaacgg
    16261 atcggtcagc gcctgcaaat gccagcggcg attaagccat tcactgcgat gataaatgcg
    16321 caccatatag agcaggcaaa cgctaaagga aatcagcacc gcaagaataa atgccagcga
    16381 atattcggtt tccacccctt gcaaaaagtt ctggttgtaa ttcagaaggc aaagcgttga
    16441 aacagccctg gtgagattta aaaacggata gcgaagctta ccgaccccga gggtaaagat
    16501 gatgaagaaa acgggtacca ggtaaccggc aataaagtcg ttttcataag gtgtgcacaa
    16561 cagaagtaga agcacgctta acgctgccag ccagcttaag gtaaatgcgc gtttctcttt
    16621 gcccaacgac ggcgcgatat ccctgcgcca caatatctgc gcaaagtggg gacttacaat
    16681 catgcgagtg agatagtaga aaagcatgtt gtaaataagc acagcggtga aaaggcttag
    16741 caaatcaacg accgtgaaaa tggcatccgc atcgccgaaa aaggtggata tctttagcgg
    16801 gaaatcaaag aagctcccca caagatacat gctgcatttg atgccgatcg gcgtcaccaa
    16861 accaagccag aacagacgtt gccagacata tcggctggtc aatccataac gccaacgcgt
    16921 ccccagctgc cagcgtaata ttgcgcaggc acacaacacg acaaacgtct ggctaccgag
    16981 catgaccgca gtctgtaaaa aagagagatg aaagttccac agattggtgc aaaacatccc
    17041 gcataaaacg ggaacgacac cgcgccagcc aaagataaac aagattgaca gcattacgca
    17101 cagtggcatc cacgccagaa agatataact ggaatggata atggcaagtg gcgaaataaa
    17161 acgggatagc tgaataagta ccacggtgag cgtaaacgct agtgtgaata tcttgatatt
    17221 ttttatcagg ttatgctcca caaacatgag tacactactc tcactgatcg gggttttcag
    17281 gcacacaaat aataagttta ttcgcatcgc gaagcaaaca gggggccagc ctgttgccga
    17341 tttttcatcc aatgatgctt tcaccaggcg gcactaaccc ggacgtagac tgtattacaa
    17401 aagcggcaaa aagcagagac aaaaaacccc cgctttgcag cgagggttgg aaatttggtg
    17461 gagctaagcg ggatcgaacc gctgacctct tgcatgccat gcaagcgctc tcccagctga
    17521 gctatagccc cgtcacgtaa agcttgtcga gttgacgggc ggcatcatat gaattccgcc
    17581 cgaatgtgtc aacggcaaat tgcaacgtgt agtttcaatc gctgaaaaat caggcaaatg
    17641 aacaattttg gaaaaccgct cgcattcagt agttattcat gtcacggttt cctgtaaagt
    17701 ggtgttataa aatgaactac taatagaccc acatacattc agggaattgt tatgttcaag
    17761 gagaggatga caccagatga acttgccaga ctgaccggct atagccgcca gaccattaat
    17821 aaatgggtac gcaaggaggg ctggacaacg tcaccaaaac ctggcgtcca gggtggcaaa
    17881 gcccgactgg ttcacgtcaa tgaacaggtt cgtgaatata ttcgcaatgc tgaacgtcca
    17941 gaaggtcagg gagaagcgcc tgccctttct ggtgatgcgc cgcttgaagt attgctggtg
    18001 acactggcga aagagatgac gccagttgaa caaaaacagt ttacatcctt gcttctgcgg
    18061 gaagggatta tcggattgtt acaacgctta gggattcgcg atagcaaata atatgaaaag
    18121 attacgcaat aaaatgacca ccgaagaact ggctgaatgc ctcggtgtgg ctaaacaaac
    18181 tgttaaccgt tggatcagag aaaaaggctg gaaaacggaa aaatttcccg gcgtgaaagg
    18241 cggtcgtgcc agacttattc tggtcgatac gcaagtttgc gagtttattc agaacacgcc
    18301 agccttccat aacacaccaa tgttgatgga agctgaagag agaattgctg aatacgctcc
    18361 tggagcacgc gccccggcgt atcgacaaat catcaacgcg atagataata tgacagacat
    18421 tgagcaggga aaagtcgcgc aatttttgtc acgtgaagga attcgcaatt tcctcgcccg
    18481 tctcgatatt gacgaatcag cataaataaa aaacggcagg atattatctc ctgccgttta
    18541 tctttttaca cgctaattac tgctgatttt cgcgttcagc aataaaatcc agcgctttgt
    18601 tgatacgctc gatactgcgg gtcttaccaa ttgcgtgaac ggtaacatcc agtgctggag
    18661 actgccccgc cccggttacg gcgacacgca gcggcatacc aactttaccc atacccactt
    18721 ccagctcatc cgccgtcgcc tgaatagcgt gatgaacgtt ttcagcggtc cagtcagtaa
    18781 tcgcggccag tttgtcacga accacttcca gcggctgacg cgctaccgga cgcagatgtt
    18841 ttttcgccgc atcggcatcg aactcagcaa aatcttcgta gaaataacgg cagctctgtg
    18901 ccatctcttt cagcgtcttg cagcgttcgc ccagcagttt caccagatca gccagctgcg
    18961 ggccgttacg ggtatcgata ttttcctgct caatgtgcca ctgtaagtga gtagcaacat
    19021 actccggcgg cagcgcgtta atgtagtgat ggttcagcca cagcagcttg tcggtgttga
    19081 acgcactggc agatttgctg acggcattca aagtgaagta tttgatcatc tcttcacgag
    19141 tgaagatttc ctgatcgccg tgggaccagc ccagacgcac cagatagttc agcagtgctt
    19201 ctggcaaata accgtcatca cgatactgca ttacgctgac tgccccgtga cgtttggaca
    19261 gttttttacc gtcatcgcca ttgatcatag aaacgtgcgc gtaaaccggc accggcgctt
    19321 tcagggcctt aagaatgttg atctggcgcg gcgtgttgtt gatatggtct tcgccacgga
    19381 taacgtgggt gatttccata tcccagtcat cgacaaccac acagaagtta taggttgggg
    19441 aaccatcggt acggcggata ataagatcgt ccagttcctg gttgctgaac tcgatcggac
    19501 cacggatctg atcgtcaaaa acaacagaac cttcctgcgg gttagcaaaa cgtacaacac
    19561 acggttcatc atcagcatga tgctcatggc tgtggcggca gcgaccgtca taacgcggct
    19621 tctcaccttt cgccatttgc tcttcgcgca gcgcttccag gcgctcttta gagcaatagc
    19681 atttataagc agtgccctct tccagcatct gatcgatcac cgcgttgtag cgatcaaaac
    19741 gtttggtctg gtagtacgga ccttcatccc actccaggct cagccagttc atgccatcca
    19801 taatggcttc gatagcttcc ggcgtggaac gctcaagatc ggtgtcttca atacgcagca
    19861 cgaactcacc gccgtggtta cgtgcaaaaa gccaggagta aagagcagta cgcgcgccgc
    19921 caacgtgcag atagcctgtt gggcttggcg cgaagcgagt tttgattttc atgaaatggc
    19981 cttacgttta gaaagatgcc gacaaccggc aaatcctgga aaaattgagt gggcgatatt
    20041 ctatcactcc cgcctgattc ctcaatgtag ttcgggtttc taccttacgc attattgttt
    20101 tcgttgacaa attgcgcaac acggtctgtt ttgcgatcat cttgcttaaa tttacgacga
    20161 acgaacaatt tctttagaaa atgcgttgac tcattttgaa ctctccctat aatgcggctc
    20221 cacacagcgg gggtgattag ctcagctggg agagcacctc ccttacaagg agggggtcgg
    20281 cggttcgatc ccgtcatcac ccaccaacat actttatgta gtctccgccg tgtagcaaga
    20341 aattgagaag tgggtgatta gctcagctgg gagagcacct cccttacaag gagggggtcg
    20401 gcggttcgat cccgtcatca cccaccaatt tctcgccagc t
//
LOCUS       DAJTZL010000069        20147 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.069, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000069 DAJTZL010000000
VERSION     DAJTZL010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20147
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.069"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..1277)
                     /locus_tag="OO544_002125"
     CDS             complement(<1..1277)
                     /locus_tag="OO544_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001324426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6767717.1"
                     /translation="MEYFMKGKSALTLLLAGIFSCGTCQATGAEVTSESVFNILNSTG
                     AATDKSYLSLNPDKYPNYRLLIHSAKLKNEIKSHYTKDEIQGLLTLTENTRKLTLTEK
                     PWGTFILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWDYM
                     STLDQIKRMQDVISNPKRLDGVPGQMNAVHIRFDSNSPVMADVQEEGKPQLWNHKQND
                     ALGLYLDLLIQAIDTGTINAEDWQKGDRLKSVALLIAYLDKANFYVMEDSGAWEEDAR
                     LNTSSVALVTSGLERLSNLLSKKDSVFVSDLLREAKANELDEPLSTTRLNHLIDKGYE
                     RITLQLDLGGESPGYLEKDKHYREADAALLNVIYPANLAKINTRRKEQVLKIVKKLAG
                     PYGIKRYEKDNYQSANFWFNDIKTDTDQNSHAK"
     gene            complement(1470..1539)
                     /locus_tag="OO544_002126"
     tRNA            complement(1470..1539)
                     /locus_tag="OO544_002126"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1505..1507),aa:Gly,seq:ccc)
     gene            complement(1618..2373)
                     /locus_tag="OO544_002127"
     CDS             complement(1618..2373)
                     /locus_tag="OO544_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HCR6767718.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            2788..5085
                     /gene="xdhA"
                     /locus_tag="OO544_002128"
     CDS             2788..5085
                     /gene="xdhA"
                     /locus_tag="OO544_002128"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HCR6767719.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALAEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGRN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFAPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPSAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            5096..5974
                     /gene="xdhB"
                     /locus_tag="OO544_002129"
     CDS             5096..5974
                     /gene="xdhB"
                     /locus_tag="OO544_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HCR6767720.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLADGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEAPITQRHLPALCAAATSIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGSAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            5971..6450
                     /gene="xdhC"
                     /locus_tag="OO544_002130"
     CDS             5971..6450
                     /gene="xdhC"
                     /locus_tag="OO544_002130"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HCR6767721.1"
                     /translation="MNHSETITIECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(6490..8268)
                     /locus_tag="OO544_002131"
     CDS             complement(6490..8268)
                     /locus_tag="OO544_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HCR6767722.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQFEVEIVDENLCRVAG
                     TGAYGKFLGRQLSGNSRLLRHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDDESRVQFVNQTALKTLGVVQNNIIGKPIRFRPLTFESNFTH
                     GHMQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGEC
                     RVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPE
                     QLLESELFGYVKGAFTGASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAR
                     EILPIGASSPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQED
                     IELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGE
                     VIDSTLLPPNLLNNGTTEQSDVTEVSEAHLSLDDAGGTALEEMEKQMIREALSRHNSK
                     KQVADELGIGIATLYRKIKKYELLNT"
     gene            8747..9934
                     /gene="ygeW"
                     /locus_tag="OO544_002132"
     CDS             8747..9934
                     /gene="ygeW"
                     /locus_tag="OO544_002132"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HCR6767723.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNSTRTRFSYASALNLLGLAQQDLDEGKSQIAHGE
                     TVRETANMISFCADAIGIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQC
                     DIDHPTQSMADLAWLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFG
                     MDVTLAHPEGYDLIPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKV
                     MEERTELLRANDHEGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADI
                     SGVSCKEGEVTEGVFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQE
                     RVK"
     gene            9992..11188
                     /gene="dpaL"
                     /locus_tag="OO544_002133"
     CDS             9992..11188
                     /gene="dpaL"
                     /locus_tag="OO544_002133"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HCR6767724.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYRP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQHQSLMEKLALDKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            11246..12457
                     /locus_tag="OO544_002134"
     CDS             11246..12457
                     /locus_tag="OO544_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HCR6767725.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQIYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            12510..13894
                     /gene="hyuA"
                     /locus_tag="OO544_002135"
                     /pseudo
     CDS             12510..13894
                     /gene="hyuA"
                     /locus_tag="OO544_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
     gene            13942..14874
                     /gene="arcC"
                     /locus_tag="OO544_002136"
     CDS             13942..14874
                     /gene="arcC"
                     /locus_tag="OO544_002136"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCR6767726.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNALREELLSRGI
                     NKPVATLVTQVEVDANDPAFLNPTKPIGSFFTEQEAEQLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVDAGQVVIAVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFARSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(14915..16540)
                     /locus_tag="OO544_002137"
     CDS             complement(14915..16540)
                     /locus_tag="OO544_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF2563 family selenium-dependent molybdenum
                     hydroxylase system protein"
                     /protein_id="HCR6767727.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIVDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVERKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EELDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILWVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSSA
                     EAMKLTERGFIPVMVDPACSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFT
                     AGKDCHAVIETNRGHWLGQVIYSGCAQENTDVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(16588..17358)
                     /gene="yqeC"
                     /locus_tag="OO544_002138"
     CDS             complement(16588..17358)
                     /gene="yqeC"
                     /locus_tag="OO544_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium-dependent hydroxylase
                     accessory protein YqeC"
                     /protein_id="HCR6767728.1"
                     /translation="MKSIVDPSALVIDLGAQKRPTVISVVGAGGKTSLLFWLAELLQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASLTSPISFCFHSWKANQGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRGMPLKAPDEHEPCIPKSSCCVIAVMGGHTLGAKV
                     STENVHRWSQFADITGLTPDATLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHDVEAIWLGDIQEHPAIARRFVN"
     gene            17461..18039
                     /gene="mocA"
                     /locus_tag="OO544_002139"
     CDS             17461..18039
                     /gene="mocA"
                     /locus_tag="OO544_002139"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HCR6767729.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMFPWEQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYANQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLMQAIQRPNVTNMRQALLMG
                     DHYSVEIENAEIILDIDTPDDFITAQKRYTKI"
     gene            18361..>20147
                     /gene="ygfK"
                     /locus_tag="OO544_002140"
     CDS             18361..>20147
                     /gene="ygfK"
                     /locus_tag="OO544_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HCR6767730.1"
                     /translation="MGDIMRPIPFEELLTRIFDEYQQQRSIFGIPEQQFYSPVKGKTV
                     SVFGETCATPVGPAAGPHTQLAQNIVTSWLTGGRFIELKTVQILDRLELEKPCIDAED
                     ECFNTEWSTEFTLLKAWDEYLKAWFALHLLEAMLQPSDSGKSFIFNMSIGYNLEGIKQ
                     PPMQQFIDNMMDASDHPKFAQYRDTLNKLLQDDAFLARHGLQEKRENLQALPARIPTS
                     MVQGVPLSTMHGCPPHEIEAICRYMLEEKGLNTFVKLNPTLLGYARVREILDVCGFGY
                     IGLKEESFDHDLKLTQALEMLERLMVLAKEKSLGFGVKLTNTLGTINNKGALPGEEMY
                     MSGRALFPLSINVAAVLSRAFDGKLPISYSGGASQLTIRDIFDTGIRPITMATDLLKP
                     GGYLRLSACMRELEGSDAWGLDHVDVERLNRLAADALTMEYTQKHWKPEERIEVAEDL
                     PLTDCYVAPCVTACAIKQDIPEYIRLLGEHRYADALELIYQRNALPAITGHICDHQCQ
                     YNCTRLDYDSALNIRELKKVALEKGWDEYKQRWHKPAGSGSRHPVAVIGAGPAGLAAG
                     YFLARAGHPVTLFEREANAGGVVKNIIPQ"
BASE COUNT         5225 a         5180 c         4764 g         4978 t
ORIGIN      
        1 cttttcgcat gtgaattttg atcggtatcg gttttaatat cattgaacca aaaatttgcc
       61 gactgataat tatccttctc gtatcttttt ataccatacg gacctgctag ttttttaact
      121 atttttagaa cctgctcttt tcttctggtg tttatctttg caagatttgc aggataaata
      181 acattaagta aagccgcatc ggcttctctg taatgtttat ctttttccag ataacctggg
      241 gattctcccc ctaaatcaag ctgtaacgtt attctttcat aacctttatc tatgaggtgg
      301 ttcaacctcg ttgttgaaag aggttcatcc agttcattgg ctttagcttc gcgtagtaaa
      361 tcagagacaa aaacagagtc tttcttcgag agtaagttcg acaaacgttc cagtccagag
      421 gtcaccagtg caacggaaga tgtgtttaat cgggcatctt cttcccatgc tccggaatct
      481 tccataacat agaaattggc tttatccaga taagcaatca gcagcgcaac cgattttaat
      541 cggtcacctt tttgccaatc ttcagcgtta attgtaccag tgtcgattgc ttgtataagg
      601 agatcaagat aaagacctaa cgcatcattt tgtttatggt tccacagctg cggcttgcct
      661 tcttcctgca catctgccat cacgggggag ttgctgtcaa atcggatatg cacagcattc
      721 atttgcccgg gaactccatc cagtcgtttg gggttgctaa tgacgtcctg catccgctta
      781 atctggtcga gcgtagacat ataatcccac agggtgagca gaaccttttt tgcggctaca
      841 ctatttccct gatctgatac taatgccata tagccccata aactatccct cagccagaca
      901 gcatcatagt gggtttctgc cgctgttttg tcatcttcga aagtagaagc aagaataaat
      961 gttccccagg gtttctccgt caacgttaac tttcgtgtgt tttctgttaa tgttaacaac
     1021 ccttgaattt cgtctttggt atagtggctc tttatttcat tttttaattt ggctgagtga
     1081 atcagaagac gataattagg gtacttatct ggatttaacg ataaataact tttatctgtt
     1141 gctgcccccg tggaattaag aatgttaaat acagattcac tcgtcacttc tgcccctgtc
     1201 gcctggcatg tgccgcacga aaatatacca gcgagcagaa gagttaatgc agatttccct
     1261 ttcataaaat attccatcaa tcgtttgtta aaagcgtaaa taaccactta tttaacaact
     1321 tgaaaataag gtgttttacc tgggttgtta caaaaggatt gcattgcgta aacgcttttt
     1381 atttacaaca aaatggggaa atattacggc gattgcgaaa agtctgaagc gattgaatta
     1441 agataatata gaaaatcaga tacagcaaaa gcggcgaagg gaatcgaacc ctcgtataga
     1501 gcttgggaag ctctcgttct accattgaac tacgcccgct tcgagatgcg taaggcatta
     1561 taaaccttac gctctcctta gcaagtgccg cgctgctgac tgctgattaa ttcgccatca
     1621 gcattttggc ttgctgccct gcggcggcaa gtaacgtagc ggatcaattg ccgttgcacg
     1681 gtaacgaatc tggaaatgca ggcgaacaga tgccgcatcc gtgctcccca tagtggcgat
     1741 tttttgccca gccttcacgc tttgcccatt atttaccagc atcgtgtcat tatgggcgta
     1801 agccgtaatg taatcttcac tgtgtttaat catgatgaga ttaccgtagc cacgcagctg
     1861 gttgcccaca tacaccacct ttcctgcacc cgcggcgtaa ataggtgtac cccgtggagc
     1921 tgagatatca atccctttat tgccgccatc tgctgtcgaa tacggcatga taactttccc
     1981 tgtcgttggc cataaccaac aacgttgccc tactggcggc caggatgatt tcggtaccgc
     2041 tgatgacggt gtaaccgatg cggttttggt cgttgatttg gcggttgatt tacgtgtact
     2101 actgctactt ttcgccccac ccagttttag tttctgacca acttcaatgg tgtaaggggg
     2161 ggaaatgccg ttcagtcgcg ccagttcttt tacgctggtt cccgtggtgc gcgaaatacg
     2221 atatagcgta tccccccgtt tcacggtgta aacggagccg gaatacgttc ctgtatcgga
     2281 tgatttgcta cccgaacagc ccgccaaaag cagtccaacc gataacaaca tcacgatacc
     2341 cagagatttt ttattcaggc gtcccgcact caaaatcttt cctcgctaaa aaaacaagac
     2401 gccttatcat agcagccagc ctcttgatac ccaatctctt gcttctggaa aaacgtggta
     2461 atagagaaag aggttaattt catttggcaa agggaagtat catcagcaaa atattcgctg
     2521 tagaggtatc gaacttcctg actttttacc ctatttccca tcctgatgcg taataatccc
     2581 aatttatcac attgatttta tcgccactca tattgatacg tatcactgcc cctattcaga
     2641 gtcctgtttc gtatcatatc tgtgtttttt taacgtcatc acacttccta cctcccctaa
     2701 cgcttatcgt cgtttctggc gtaaatcttg cctgcttaga ctaaatcttt gccataagaa
     2761 ccaaatgttc aaatcgaggg gatttctatg gaagcgcggg aagcaaccgc tacgggtgaa
     2821 tcatgcatgc gcgtcgatgc cattgctaag gtcaccgggc gggcacgata tactgacgat
     2881 tatgttatgg cgggcatgtg ttatgcgaaa tatgtacgta gccctatcgc acatggttat
     2941 gccgtaagta ttaatgatga acaagccaga agtttgccgg gcgtactggc gatttttacc
     3001 tgggaagatg tgcctgatat tccattcgct acagctgggc atgcctggac acttgacgaa
     3061 aacaagcgcg ataccgccga tcgcgcactg ctaactcgcc atgttcgtca tcatggtgac
     3121 gccgttgcca tcgtcgtggc ccgcgatgaa ctcacggcag aaaaagcggc gcaattggtc
     3181 agcattgagt ggcaagaatt acccgttatc accacgccag aagcggcgct ggcagaagac
     3241 gctgcaccaa tccataacgg tggcaattta ctgaaacaaa gcacgatgtc gacgggtaat
     3301 gtccaacaaa caatcgatgc cgccgactac caggtacagg ggcactatca gacccccgtt
     3361 attcaacatt gtcacatgga aagcgtaaca tcgctggcgt ggatggagga tgactcgcga
     3421 attaccatcg tttccagcac ccagatcccg cacattgttc gccgcgtggt tggtcaggcg
     3481 ctggatattc cctggtcatg cgtacgagtc atcaaaccgt ttgtcggtgg cggttttggt
     3541 aataaacagg atgtactgga agagccaatg gcagcattcc tgaccagcaa gcttggcggc
     3601 attccggtga aagtttccct tagccgtgaa gagtgtttcc tcgcaacccg tacccgccac
     3661 gcttttacca ttgacgggca aatgggcgtg aaccgcgacg gaacattgaa aggttatagt
     3721 ctggatgttc tgtctaacac cggcgcttat gcatctcacg ggcactccat cgcttctgcg
     3781 gggaggaata aagtcgctta cctttatcct cgttgtgcct acgcttacag ttcaaagacc
     3841 tgctatacca acctcccctc ggctggtgcg atgcgtggtt atggcgcgcc acaagtcgta
     3901 tttgccgttg agtctatgct tgatgacgcc gcgacagcgt taggtattga tcctgttgaa
     3961 attcgtttac gcaacgccgc ccgcgaagga gatgctaatc cgctcacggg caaacgtatt
     4021 tacagcgcag ggttgccgga gtgtcttgaa aaaggccgga aaatctttga atgggaaaaa
     4081 cgccgtgcag aatgccagaa ccagcaaggc aatttgcgcc gcggcgttgg cgtcgcctgt
     4141 tttagctata cctctaacac ctggcctgtc ggcgtagaaa tagcaggcgc gcgccttctg
     4201 atgaatcagg atggaaccat caacgtgcaa agcggcgcga cggaaatcgg tcagggtgcc
     4261 gacaccgtct tctcgcaaat ggtggcagaa accgtggggg ttccggtcag cgacgttcgc
     4321 gttatttcaa ctcaagatac cgacgttacg ccgttcgctc ccggcgcatt tgcctcacgc
     4381 cagagctatg ttgccgcgcc tgcgctgcgc agtgcggcac tattattaaa agagaaaatc
     4441 atcgctcacg ccgcagtcat gctacatcag tcagcgatga atctgaccct gataaaaggc
     4501 catatcgtgc tggttgaacg accggaagag ccgttaatgt cgttaaaaga tttggcgatg
     4561 gacgctttct accaccctga acgcggcggg cagctctctg ctgaaagctc catcaaaacc
     4621 accactaacc catcagcgtt tggctgtacc tttgttgatc tgacggtcga tattgcgctg
     4681 tgcaaagtca ccatcaaccg catcctcaac gttcatgatt cagggcatat tcttaatcca
     4741 ctgctggcag aaggtcaggt acacggcgga atgggaatgg gcattggctg ggcgctattt
     4801 gaagagatga tcatcgatgc taaaagcggc gtggtccgta accccaatct gctggattac
     4861 aaaatgccga ccatgccgga tctgccacaa ctggaaagcg cgttcgtcga aatcaatgag
     4921 ccgcaatccg catacggaca taagtcactg ggtgagccac caataattcc tgttgccgct
     4981 gctattcgta acgcggtgaa gatggctacc ggtgttgcaa tcaatacact gccgctgacg
     5041 ccaaaacggt tatatgaaga gttccatctg gcaggattga tttgaggata acatcatgtt
     5101 tgattttgct tcttaccatc gcgcagcaac ccttgccgat gccatcaacc tgctggctga
     5161 caacccgcag gccaaactgc tcgccgatgg cactgacgta ctgattcagc tccaccatca
     5221 caatgaccgt tatcgccata ttgttgatat tcataatctg gcggagctgc ggggaattac
     5281 gctggcggaa gatggctcgc tacgtatcgg ctctgcaacg acatttaccc agctaataga
     5341 agctcctata actcaacgtc atctcccggc gttatgtgct gcggccacgt ccattgctgg
     5401 accgcagatc cgtaacgtcg ctacctacgg tggaaatatt tgcaacggtg ccaccagcgc
     5461 agattctgcc acgccaacgc taatttatga cgcgaaactg gagatccact ccccgcgcgg
     5521 tgttcgtttc gtcccgatta atggctttca caccgggccg ggcaaagtgt ctcttgagca
     5581 tgacgaaatc ctcgtcgcct ttcattttcc gccacagccg aaagaacacg cgggcagcgc
     5641 gcattttaaa tatgccatgc gcgacgcaat ggatatttca acgattggca gcgccgcaca
     5701 ttgccgactg gataacggca atttcagcga attacgcctg gcatttggtg ttgccgcgcc
     5761 aacgccgatt cgctgccaac atgccgaaca gactgcacaa aatgcgccat taaacctgca
     5821 aacgctggaa gctatcagcg aatctgtcct gcaagatgtc gccccgcgtt cttcatggcg
     5881 ggccagtaaa gagtttcgtc tgcatctcat ccagacgatg accaaaaaag tgattagcga
     5941 agccgtcgcc gcggcggggg gaaaattgca atgaatcaca gcgaaacaat taccatcgaa
     6001 tgcaccatta acgggatgcc ttttcagctt cacgccgcac caggcacgcc gctctcggaa
     6061 ttactccgcg aacaaggact gctaagtgtc aaacaagggt gctgcgtggg tgaatgtggt
     6121 gcctgtacgg tgttggtcga cggcacagca atagacagtt gcttatacct tgccgcctgg
     6181 gctgaaggaa aggagatccg cacgctggaa ggtgaagcga aaggcggaaa actttctcat
     6241 gttcagcagg cttatgcgaa atccggcgca gtgcagtgcg ggttttgtac gcctggcctg
     6301 attatggcta ccacggcaat gctggcgaaa ccacgcgaga agccattaac cattacggaa
     6361 attcgtcgcg gactggcggg aaatctttgt cgctgcacgg ggtatcagat gattgtaaat
     6421 acagttctgg attgcgagaa aacgaagtaa aaggatatcc ggcctgaatt caggccggat
     6481 tcactgaggt tatgtgttta acaactcata tttcttaatc ttgcgataga gcgtagcaat
     6541 gccgatgccc agttcatcag caacttgctt cttgctgtta tgacgtgaaa gcgcctcgcg
     6601 gatcatttgc ttttccatct cctccagcgc cgtgccgccc gcatcatcga gtgacaggtg
     6661 cgcctcactg acctctgtta catcactttg ctccgttgtg ccattattca gcagatttgg
     6721 cggcaatagc gtgctgtcga taacttcacc tgaaggaacc acgttaacca gatattccat
     6781 caaattgctt aactcgcgca ggtttccggg ccaacgatgc ttacgcaata tttcgacgac
     6841 atcgggagca atgccaggat aaaccgatcc cagacgacgg gtatgcagat gtaaaaagta
     6901 atgcaccaat agttcaatat cttcctgacg ttcacgcagc ggtggcagag ttatcgggat
     6961 aacattaagt cggtagaaga gatcttcgcg gaatttacct tcggcaatga actgggccaa
     7021 attctgatta gttgcagaaa tgatgcgaat gtcgacttgt attgggctac tggcaccaat
     7081 cggcagaatt tcacgcgcct caatagcgcg cagtaattta gcctgcaaca ttaatggcat
     7141 atcacctatt tcatcgagaa acagcgtgcc cgtattcgcc gcctgaatca accctgtttt
     7201 accgttggca gaagcaccag taaatgcgcc tttaacataa ccgaacagtt cgctttccag
     7261 aagctgctcc ggaatcgcgg cacagttgat agcaataaag ggtttattcc gtcttccgct
     7321 caacttatgg attgctcggg cgacgacttc tttacccgtg ccgctttcac caaccaccat
     7381 aacgctggat gggctgggtg caatacggct aatgagtcgt tttaattgcc gcataacacg
     7441 gcactcgcca accaattgtt caatatgtgg ttcatcaggt gcatttgcta cagaaaaact
     7501 ggtatgcgat tggtgaaatg ccattaaaaa taattgtcgg ccctgaatgt tatgcaattg
     7561 accaatgatt aattcacttt tatcgtccca cgaaacaata tgctgcatat gtccatgagt
     7621 aaaattactc tcaaatgtta atggtctgaa acggataggt ttcccaataa tattattttg
     7681 tacaacacca agtgttttta aggcagtctg attaacaaac tgaacccgac tttcatcatc
     7741 aacaactaat acgccctgat ccatattatc gatcatggtc gcgaatattt tactgatgtt
     7801 atctcctggc ccctgatcct ccagaagttt cgaaacaaaa atggtggata tatggcgaac
     7861 ataatcagaa aattcgcgta aattatcact gatatgctct tgttgctcgt gggtaacggc
     7921 aatcaaactt atcaccccaa cacaacgatc ctgtaaaatg acaggcgtac ccagaaatgc
     7981 tttttcgcgg caattttctt tactatcgca accttcgcaa aggggatcga agcgagactg
     8041 tgtcacaact ttttcagttt tcgtttccag gacgtggcgg agcaggcgtg agttgccgct
     8101 caactggcga ccaagaaact tcccatacgc gcccgttccg gcgacgcgac acaagttttc
     8161 atcaacgatc tcaacctcaa actgcaaaac gctggcaagc attctggcaa aacgctgaat
     8221 tgtcggttga atttgcatca atactgactg cgtagtagca agctccatag ctttaccttc
     8281 cagacttact taaaagtcga tcattgaaga cgttgatggt tcacagatca tgatgatatt
     8341 aactcaggcg aaattggctt tgataaaaac ataagatttt tatcattttc taatgcaatt
     8401 atggaagaga tatcacattt ctatatcaat atgagaatta cggcggtgag tttatcaaac
     8461 tgaagagaga tagcctgccc ctttatctta tttctgatac ttagcagaaa ataaataacg
     8521 cgataaaaaa agccaaacgt tttcgtattt tacaaacaac cagaagctgg catcaatttg
     8581 tgaacaaccc cacacattat ccgtcaaatt agtcttttgc agccgcgcgg ataattctgg
     8641 cacacttatt gttagtccca ggtatagcta tgaaaacacc aatcactttg gcaagtcaca
     8701 gtgaaataaa ccactttgcc tgtcattcca ctaccgggac tgtatgatga aaactgttaa
     8761 tgagctgatt aaggatatca attcgctgac ctctcacctt cacgagaaag attttttgtt
     8821 aacgtgggaa cagacgccag atgaactgaa acaagtactg gacgttgccg cagcattaaa
     8881 agcactgcgt gctgaaaaca tctcaaccaa agtctttaat agtggattag gtatttccgt
     8941 attccgcgac aactccaccc gtacccgctt ctcttatgct tccgcgctta acctgctcgg
     9001 ccttgcacaa caagatctcg acgaaggcaa atcacaaatc gctcacggcg aaaccgtgcg
     9061 tgaaaccgcc aatatgatct ccttctgcgc cgacgctatt ggtattcgcg acgatatgta
     9121 tctgggcgca ggcaacgcct atatgcgtga agttggcgct gcacttgatg acggttacaa
     9181 gcagggtgta ctgccacagc gtccggcttt agtgaacctg caatgcgata ttgaccaccc
     9241 gactcagtca atggctgacc tcgcgtggtt acgtgaacac tttggttcac tggaaaacct
     9301 gaaaggtaaa aaaatcgcca tgacctgggc ctactctcca agctatggca aaccgctctc
     9361 tgtaccacaa ggcatcatcg gtctgatgac tcgcttcggt atggatgtca ccttggccca
     9421 tccggaaggc tacgacctga tcccggatgt ggttgaagtg gcgaaaaaca atgctaaagc
     9481 ctccggtggt agcttccgtc aggtcaccag catggaagaa gccttcaaag acgcagacat
     9541 cgtttatccg aagtcatggg caccttacaa agtgatggaa gagcgtactg aattgctgcg
     9601 tgcgaacgac cacgaaggct taaaagcact ggaaaaacag tgtctggcac agaacgcgca
     9661 acacaaagac tggcattgta ctgaagagat gatggaactg acccgtgatg gcgaagccct
     9721 gtacatgcac tgcctgccag ctgatatcag cggcgtatcc tgcaaagaag gtgaagtgac
     9781 tgaaggcgta ttcgaaaaat accgtatcgc tacctacaaa gaagccagct ggaagcctta
     9841 tatcatcgcc gcgatgatcc tgtcccgtaa atacgccaaa ccaggtgcac tgctcgagca
     9901 actgctgaaa gaagcgcaag aacgcgtgaa ataacccctc gggccgatct gctggtcggc
     9961 cctctatttc cagaggccaa aaggatagga tatgtccgtt ttctcattga agattgatat
    10021 cgccgataac aaatttttca acggcgaaac atcaccgctc ttttcgcaaa gccaggccaa
    10081 actggcgcgc cagttccacc agaaaatagc tggttatcgc ccaacaccgc tttgtgcgct
    10141 ggacgatctc gcaaaccttt ttggtgtgaa gaaaattctc gtcaaagacg aatcaaaacg
    10201 attcggtctg aacgccttca aaatgcttgg cggtgcgtac gccatcgctc aattattgtg
    10261 tgaaaaatat catcttgata tcgaaacgct gtcatttgag cacctgaaaa atgccatcgg
    10321 cgaaaaaatg actttcgcga ccaccaccga cggcaaccac gggcgcggtg tggcgtgggc
    10381 agcacagcaa ctcggacaga atgcggtgat ttacatgccg aaaggttctg ctcaggaacg
    10441 cgttgacgcc attctgaacc tcggtgccga gtgcatcgtc acggatatga actatgacga
    10501 taccgttcgc ctgaccatgc aacacgcgca gcagcacggc tgggaagtgg tacaggacac
    10561 ggcatgggaa ggttacacca aaatcccaac ctggatcatg caaggctacg caaccctggc
    10621 agatgaagcc gtcgagcaaa tgcgtgaaat gggcgtaacc ccgacgcacg ttctgctgca
    10681 agccggtgtc ggagcaatgg ccggtggtgt gctgggttat ctggtcgacg tctacagccc
    10741 gcaaaatctg cacagcatta ttgttgaacc tgacaaagct gactgtattt atcgctccgg
    10801 cgtcaaaggc gacatcgtca acgttggcgg tgatatggcc accatcatgg cgggcctggc
    10861 ctgtggcgaa cctaacccgc tgggctggga aatcctacgt aactgcgcca cccaattcat
    10921 ctcctgccag gacagcgttg ccgcattagg tatgcgcgtg ctgggtaatc cgtacggcaa
    10981 cgacccgcgc atcatctccg gtgaatccgg cgctgtcggt ttgggcgttc tcgcagcggt
    11041 tcattatcac ccgcaacatc aaagcctgat ggaaaaactg gcgctggaca aagatgccgt
    11101 agtgctggtt atcagcactg aaggcgacac cgacgtgaag cactaccgcg aagttgtctg
    11161 ggaaggcaaa cacgctgtag caccttaatc accctattgg aactactccc tgcaaaacgg
    11221 ggagtaaaaa atctggagaa aaataatggc taagaatatt ccattcaaac tgattcttga
    11281 aaaagcaaaa gattaccagg cggacatgac tcgcttcctg cgcgacatgg ttgctattcc
    11341 cagtgaaagc tgcgacgaaa aacgcgtagt acatcgtatt aaagaagaga tggaaaaagt
    11401 cggcttcgat aaagttgaaa tcgacccgat gggcaacgtt ctcggttata tcggccacgg
    11461 cccgcgtctg gtggcaatgg acgctcatat cgataccgtc ggcattggca acatcaaaaa
    11521 ctgggacttc gatccgtacg aaggcatgga aactgatgag ctaatcggtg gtcgcggtac
    11581 ttccgaccag gaaggcggca tggcatctat ggtttatgcc ggtaaaatca ttaaagacct
    11641 cggtctggaa gatgaatata ccctgctggt taccggtact gtgcaggaag aagactgcga
    11701 cggtctgtgc tggcagtaca ttattgaaca atccggcatt cgcccggaat ttgtggtcag
    11761 taccgaacca accgactgcc agatataccg tggtcagcgc ggtcgtatgg aaattcgtat
    11821 tgatgttcag ggtgttagct gccacggttc tgcgccagaa cgcggtgaca acgccatttt
    11881 caaaatgggt ccgattcttg gcgaattaca agaactctcc caacgtctgg gttatgacga
    11941 attcctcggc aaaggcaccc tcaccgtttc tgaaatcttc ttcacatccc caagccgttg
    12001 cgctgtagca gatagctgcg ccgtctctat tgaccgccgt ctgacctggg gcgaaacctg
    12061 ggaaggcgcg ctggacgaaa tccgcgccct gcctgcagta cagaaagcta acgcggttgt
    12121 ttctatgtac aactacgacc gtccgtcctg gactggcctg gtttacccaa ccgaatgcta
    12181 cttcccgacc tggaaagtgg aagaagatca cttcaccgtt aaagcactgg tgaatgccta
    12241 cgaagggctg tttggcaaag cgccggttgt tgataagtgg accttctcaa ctaacggcgt
    12301 atctatcatg ggccgtcacg gcattccggt gatcggcttt ggcccgggta aagaacctga
    12361 agcgcatgca cctaacgaaa aaacctggaa atctcacctg gtgacctgtg ccgcgatgta
    12421 cgctgcaatc ccgttaagct ggctggcaac agagtaatta ctcttcactt attcccctcc
    12481 ggttcgccgg agggtttttg gagtttgcta tgcgcgtatt gatcaaaaac ggcactgtcg
    12541 ttaacgcaga tggacaagcc aaacaggatt tgctgattga aagcgggatt gttcgccagt
    12601 tgggcaacaa tatttcgccg cagctcccgt atgaagaaat tgatgccact ggctgttacg
    12661 ttttccctgg cggcgtggat gtccatacgc atttcaatat tgatgtcggc atcgcgcgca
    12721 gttgtgatga ttttttaccg gtacccgcgc agctgcgtgt ggcggtacaa caaccattat
    12781 tgaccatatg ggatttggcc caaacggctg tcggttacgc catcaactgg aggtttatcg
    12841 tggttatgcc gcccataaag cggtcatcga ttacagcttt cacggtgtga tccagcacat
    12901 taatcacgca atcctcgacg aaatcccgat gatggtcgag gaaggactga gcagttttaa
    12961 actctattta acctatcaat acaaactcaa cgatgacgag gttttgcagg cattacgccg
    13021 cttgcatgaa tccggcgcgc tgaccaccgt gcacccggaa aatgatgcgg ctatcgccag
    13081 caagcgggcg gaatttatcg ccgcagggtt aaccgcgccg cgctatcatg ccttgagtcg
    13141 ccctctggaa tgcgaagcgg aagccatcgc ccgcatgatt aacctggcac aaattgccgg
    13201 taacgccccg ctctatatcg tgcacctgtc taacggctta ggtctggatt atctgcgtct
    13261 tgcccgtgcg aatcaccagc cagtctgggt tgaaacctgc ccacaatatc tcctgttgga
    13321 cgaacgcagt tacgatacag aagatggcat gaagttcatt cttagcccac cgctgcgtaa
    13381 cgtacgcgag caggacaaac tgtggtgtgg catcagcgat ggtgcgattg acgtggtggc
    13441 gaccgatcac tgcaccttct cgatggctca acgcctgcaa atttctaaag gcgatttcag
    13501 tcgctgccca aatggcttac ccggtgtgga aaaccgcatg cagttactgt tttccagtgg
    13561 cgtgatgacg ggacgtataa caccggaacg ctttgttgaa ttaaccagcg caatgcccgc
    13621 caggctgttt ggcctgtggc cgcaaaaagg attattagcg cccggttccg atggcgacgt
    13681 ggtgattatc gacccacgtc agagccaaca aattcagcat cgccatctcc acgacaacgc
    13741 cgactactcg ccatgggagg gttttacctg tcagggcgcg attgtcagaa ccttatcccg
    13801 tggtgaaacg attttctgtg acggcacctt tacaggcaaa gccgggcgag gtcgtttcct
    13861 gcgacgcaaa ccgtttgtcc ctcccgtgct ctaaccactg tcagggatct atacacaatc
    13921 aagaatgatg agtgaggaaa aatgagtaag aaaattgttc tcgccctggg cgggaatgcg
    13981 ctgggcgatg acctggccgg acaaatgaaa gcggtaaaaa ttacttctca ggcaattgtt
    14041 gatttaattg cccaaggaca tgaagttatc gtcactcatg gtaacgggcc gcaggtgggc
    14101 atgattaacc aagcctttga agctgccgca aaaactgaag cacactcgcc aatgctgccg
    14161 atgtctgtct gtgttgcctt aagccagggt tatattggtt acgatctaca aaacgcgtta
    14221 agagaagaac tgctttctcg gggcattaat aaacctgtag caacgctggt tactcaggtc
    14281 gaagtcgacg ctaacgatcc ggcattcctc aacccgacca agccgatcgg ttcgttcttt
    14341 accgagcaag aagcagagca actgacaaaa cagggttaca ctctgaaaga agatgctggt
    14401 cgcggctatc gccgtgttgt tgcctcgcca aaaccggttg atatcattga aaaagaaacg
    14461 gttaaagctc tggtagatgc aggccaggtg gtgatcgccg ttggcggtgg tggtatccct
    14521 gttattcgtg aaggtaacca tctgcgtggt gccagcgcgg ttatcgataa agactgggcc
    14581 agcgcccgtt tagcagaaat gattgatgcc gatatgctga tcattctgac tgcggtagaa
    14641 aaagtggcca ttaacttcgg taaagagaat gaacagtggc tcgatcgcct gtcattaagt
    14701 gatgctgaac gctttatcga agaaggtcat tttgcgaaag gttctatgct gcctaaagtg
    14761 gaagctgccg cctcgtttgc tcgctctcgc gcaggtcgtg aagcactgat taccgtattg
    14821 agcaaagcga aagaagggat tgaaggcaaa accggaacgg tgatttgtca gtaattttaa
    14881 ggccgggtgg gtaataccat ccggccattt tcatttaagc cacttccagc acttcttttg
    14941 tcgctttcac gccctgatgc atcaacatca ttaacgcctc aagtacgccg ccgccaatcg
    15001 cccgggcttt atcagaaacg ctggtgaaat cagcggtttc accacgagga tcgatatcac
    15061 caattttaaa accaccaacc actgccaggc cgtcgttcaa caagccacgc accatccccg
    15121 tcaacggtgc tttaatttca tgctcaccaa tccaggcaat cacatcgccc tctttcacca
    15181 gatcgcctaa tttcacgttg gatcgcataa tgcctgcagc aggagcacgg atcacccgtc
    15241 gggtggtatg ccccataata ttgccaggaa catcggtatt ctcctgcgca caaccagagt
    15301 aaatcacctg accgagccag tgcccgcgat ttgtttcaat taccgcatga caatccttcc
    15361 ctgcagtaaa gcccggccca agcgcgattg ttactggtgc catatctgcc cgcgttccca
    15421 aattctgttt cgccagaata gcgtccacca cgcaaagcgg tttcagttca tcaagcaatg
    15481 aacaggcggg atctaccatc acagggatga atccgcgttc ggtaagtttc atcgcttccg
    15541 cagagctggt tgccaggcga gcagtgacgc cttcgaccgt catttcgcca tcgaacacgg
    15601 cctgggcaaa cgccacggta caacgaatca ctgtcggttt ttccacttcc agcatgatca
    15661 ctttaaaacc cgcatgatac agacgtagcg ccacaccact ggcgatatct cccgcaccgc
    15721 gaatcaccac cagctggtgt ccggaaggat gagaaggttt catcatcagc ccacccggcg
    15781 cgttattttt cacccataat atttctgcca gcacgctgat ggcgatctcc tgcggcgttt
    15841 ccgcacctat gttgtaacca acgggcgcat gtaaacgggc aatatgttct tcagccacgc
    15901 ctttctcacg caattgacgc aggaaaagct gaaccttgcg gcgacttgcc agcaacccca
    15961 accatgcaat gggctgttca atgagtttgt cgagggcttc acgatcctga ttattcgtgg
    16021 caatcaggac aaaattatca gggcgaatat ccagttcttc cactgccgca ccaaacgact
    16081 cagcatggag aagcgtggtt gatggtggga atagttcagg attgaggctt tcgcgataaa
    16141 tatcggcaac ggcaatatca aatcctaata gcgccgcact ctgggctatc gcccggttga
    16201 catgccccgc gccaatcaac accagacgtg gacgcatacc atggacgctg ataaatactg
    16261 acattgcacc tccacaatct gacccgacag catccgcacc gttacgagcc atacgtccat
    16321 ggaataatcg cggcttacgt tcctgcaatg cctgaagtga ctcttcaatc accttccgct
    16381 caaccattcc gccaccaatt gtaccgacga tagaaccatc ggcgcgcact aacatttgtg
    16441 cagaatggcg gggagtcgag cctcggctat caacaatttg cgccatcgca aacggacaat
    16501 tttgctcttc gagttttgca gcctctgtga aaatattcat accaacctca atggatatcc
    16561 tttcagtaac ccggaatacc cgggccgcta attcacaaat ctgcgcgcga ttgcaggatg
    16621 ttcttgtata tcgcccagcc agattgcctc tacgtcgtgt tgttgcagcg gttgaaggag
    16681 ctcgctttgt gcaatcgcat tctcacattg agaaaaacgg ttaatgaacc agactcgccg
    16741 acaaccttgc ggtacgtttt taaacgcccc ctgagggtgg cgaaccagcg caacgagatc
    16801 gctcagttgc aaggttgcat caggtgttaa cccggtaata tcagcaaact gcgaccagcg
    16861 atggacattt tctgtgctca ctttcgcgcc caaagtatgt cctcccatca cagcaatcac
    16921 gcagcaactg cttttgggta tgcaaggttc gtgctcatca ggcgctttta acggcattcc
    16981 acgcgagcca tctgcttcaa tgagaattac gtcacactct ggtcgttgca ccagtgcatc
    17041 aatcgcttct ggcgtaaatc cctgcacttt tccctggttc gctttccagc tgtgaaaaca
    17101 aaatgaaatg ggggatgtaa gagacgcatg aggaagcatg gcgggatcac gacagaaaac
    17161 cacgggccag tgagacgttg gcataaacat atgtgtcgtc gtcgtgatta acacacgcct
    17221 gccgctagcc tggagcagtt ctgccagcca aaaaagcaag ctggttttcc ccccagcacc
    17281 aacaacagaa atcacagtgg gacgtttctg cgcacctaaa tcaatgacta atgccgatgg
    17341 gtcaactata cttttcacga gtctttatga cctctctggg ataaattatc cccaacttaa
    17401 tccatcagga agtaacgcaa ttatcaggcg ttattagccc ctataaataa tggaaccact
    17461 atgtcagcca tcgactgtat aattaccgct gccggattat catcaagaat ggggcaatgg
    17521 aaaatgatgt tcccctggga acagggaaca attcttgata caagtatcaa aaatgcgttg
    17581 cagttttgta gccgaattat tttagtcacc ggctatcgtg gtaatgaact gcacgaacgt
    17641 tatgcgaacc agagcaatat cactattatt cacaacccag attatgcgca gggtttacta
    17701 acatccgtca aggccgcagt acccgcagtg caaacagaac attgttttct cacccacggt
    17761 gacatgccaa ccctcaccat cgatattttt agaaaaatct ggtcgttacg aaatgatggc
    17821 gcaatactgc cactccataa tggcatcccc ggccatccga ttttagtgtc aaaaccatgc
    17881 ctgatgcagg caatccagcg acccaatgtc accaatatgc gtcaggcact ccttatggga
    17941 gatcattatt ccgtcgaaat agaaaatgca gaaataattt tagatattga tactccggac
    18001 gactttatta cagcgcaaaa aaggtatact aaaatttaga aaatgtagtt aaacgattgc
    18061 gttcaaatat ttaatctctc cagcactctt tatcataatg ataaggaatc actgattttg
    18121 agaaaagaga aaaagtgata aatgggctga caataataat tttcaggagt gattttaatt
    18181 ctcatttggc acctgtgagt tcattcacaa aaagccttta agtttcgagc cacctcacat
    18241 ttttttatat ttcccgccaa acctggcaag agtggtgcga ttgttgctct atccccctaa
    18301 accaccggat ttctcaacac cggtcactca atgatatctg tataagctaa ggagagggtt
    18361 atgggggata ttatgcgtcc cattccgttt gaggaacttt tgacgcgcat atttgatgaa
    18421 taccaacaac aacgctcaat ctttggtatt cccgagcaac agttttactc acctgtaaaa
    18481 ggtaaaactg ttagcgtctt cggtgaaacc tgtgccactc ccgtcggccc tgccgctggc
    18541 ccgcacacgc agctcgcgca aaatattgtc acttcctggc tgactggcgg acgcttcatc
    18601 gaactaaaaa ccgtccaaat tcttgaccgc ctggagctgg aaaagccctg tattgatgcc
    18661 gaagacgaat gctttaacac cgaatggtct accgaattta ccttgcttaa agcctgggat
    18721 gaatacctca aagcctggtt tgccctgcac cttctcgaag cgatgttaca gccttctgat
    18781 tccggtaaat cgttcatctt taatatgagc atcggttaca acctcgaagg tattaagcaa
    18841 ccgccgatgc aacagttcat cgacaatatg atggacgcat ctgaccatcc gaaattcgct
    18901 caatatcgcg atacgctgaa taaattactc caggatgacg catttttagc ccgccacgga
    18961 ttgcaggaaa aacgcgaaaa cttgcaagcc ttacccgctc gcatccccac cagtatggtg
    19021 cagggcgtcc ccctctccac catgcacggc tgtcctccgc atgaaatcga agccatttgc
    19081 cgctacatgc tggaagaaaa agggctcaac acctttgtca aacttaaccc gaccttactg
    19141 gggtacgcgc gtgttcgtga gatcctcgat gtctgcggtt tcggttacat aggcttaaaa
    19201 gaagagtcat ttgatcacga cctcaagctg acgcaagcac tggaaatgct ggagcgcctg
    19261 atggtactgg caaaagaaaa atcactcggc tttggcgtaa aactgactaa cactctcggc
    19321 accatcaaca ataaaggcgc actgcctggt gaagagatgt atatgtcagg ccgtgcgctg
    19381 ttcccgctct ccatcaatgt tgcagcagtt ctctctcgcg cctttgacgg caaactgccc
    19441 atttcttatt ccggtggtgc cagtcagctg actatccgcg atatttttga tacaggtatt
    19501 cgccctatta ctatggcaac cgacctgctg aaacctggcg gctatctgcg cttaagtgcc
    19561 tgcatgcgcg agctggaagg ctccgacgcc tggggacttg accatgttga cgtcgaacga
    19621 ctgaacagac tggcagcaga cgcgttaacc atggaataca cccagaaaca ctggaagcca
    19681 gaagagcgta ttgaagtggc agaagacctg ccgctgaccg attgctacgt tgccccctgt
    19741 gttactgcct gcgctatcaa gcaagatatt ccggaataca tccgtctgct tggcgaacac
    19801 cgctatgccg acgcgctgga actcatctac caacgcaacg ccctacctgc cattaccggt
    19861 catatttgcg atcaccagtg ccaatacaac tgtacccgcc tggattacga cagtgcgctg
    19921 aatatccgcg aactgaaaaa agtcgcgctg gaaaaaggtt gggatgaata taagcaacgc
    19981 tggcacaaac ccgccggttc tggttcacgc catccggttg ccgtgattgg tgcaggtccg
    20041 gcgggtctgg cagcaggtta cttccttgcc agagcgggcc atccggttac gctgtttgaa
    20101 cgcgaagcca atgctggcgg cgtggtgaaa aatatcattc ctcagtt
//
LOCUS       DAJTZL010000070        19922 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.070, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000070 DAJTZL010000000
VERSION     DAJTZL010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19922
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.070"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..673)
                     /locus_tag="OO544_002141"
     CDS             complement(<1..673)
                     /locus_tag="OO544_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter"
                     /protein_id="HCR6767731.1"
                     /translation="MRNKLSFDLQLSARKAAIAERITAHKIARSKVSVFLMAMSAGVF
                     MAIGFTFYLSVIADAPSSQALTHLVGGLCFTLGFILLAVCGTSLFTSSVMTVMAKSRG
                     VISWRTWLINALLVACGNLAGIACFSLLIWFSGLVMSENAMWGVAVLHCAEGKMHHTF
                     TESVSLGIMCNLMVCLALWMSYCGRSLCDKIVAMILPITLFVASGFEHCIANLFVIPF
                     AIAIRH"
     gene            complement(695..2707)
                     /gene="hyfR"
                     /locus_tag="OO544_002142"
     CDS             complement(695..2707)
                     /gene="hyfR"
                     /locus_tag="OO544_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HCR6767732.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPL
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRHAVLLGSSGMAEL
                     RFIRTHGCRFTPQDCTLFNWLARIITPVLQSWLNDEEQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PRNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLLLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADAWRSMTDLQESLQQENHQL
                     SEQLLSNLGVGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRRDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRSIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSHLLPTLNEDSALRSSMAQLLHPTTPENDEEER
                     QRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQRLGLSVREVL"
     gene            complement(2737..3150)
                     /gene="hyfJ"
                     /locus_tag="OO544_002143"
     CDS             complement(2737..3150)
                     /gene="hyfJ"
                     /locus_tag="OO544_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HCR6767733.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFPCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVQEPAIYLMARKIA"
     gene            complement(3143..3901)
                     /gene="hyfI"
                     /locus_tag="OO544_002144"
     CDS             complement(3143..3901)
                     /gene="hyfI"
                     /locus_tag="OO544_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HCR6767734.1"
                     /translation="MSPVLTQHVSQPITLDEQTQKMKQHLLQDIRRSAYVYRVDCGGC
                     NACEIEIFAAITPVFDAERFGIKVISSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            complement(3898..4437)
                     /gene="hyfH"
                     /locus_tag="OO544_002145"
     CDS             complement(3898..4437)
                     /gene="hyfH"
                     /locus_tag="OO544_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HCR6767735.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACPANALTIQTDDQQNSRTWQLYLRRCIYCGRCEEVCPTRAIQLTNNFELTVTNKA
                     DLYTRATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPECKQ
                     RATLLNDDTDVPLVAKEQL"
     gene            complement(4447..6162)
                     /gene="hyfG"
                     /locus_tag="OO544_002146"
     CDS             complement(4447..6162)
                     /gene="hyfG"
                     /locus_tag="OO544_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
                     /protein_id="HCR6767736.1"
                     /translation="MNVNSSSNRGEAILAALKTQFPGAVLDEERQTPEQVTITVKINL
                     LPDVVHYLYYQHDGWLPVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSR
                     EFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQRRLVLPDNWPEDMHPLRKDAMDYRL
                     RPEPTTDTETYPFINEGNSDARVIPIGPLHITSDEPGHFRLFVDGEQIVDADYRLFYV
                     HRGMEKLAETRMGYNEVTFLSDRVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSI
                     LLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELLTGSRKTYGLNLIGG
                     VRRDILKEQRLQTLKLVREMRADVSELVEMLLATPNMEQRTQGIGILDRQIARDYSPV
                     GPLIRGSGFARDLRFDHPYADYGNIPKTLFTFTGGDVFSRVMVRVKETFDSLAMLEFA
                     LDNMPDTPLLTEGFSYKPHAFALGFVEAPRGEDVHWSMLGDNQKLFRWRCRAATYANW
                     PVLRYMLRGNTVSDAPLIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYKEIERYGIDRN
                     RSPLK"
     gene            complement(6152..7732)
                     /gene="hyfF"
                     /locus_tag="OO544_002147"
     CDS             complement(6152..7732)
                     /gene="hyfF"
                     /locus_tag="OO544_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HCR6767737.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLESAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHILNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALAGMPPFNIFLSEFMTITAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQ
                     AVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRSTW
                     HDFLPSGTASVSEKHSER"
     gene            complement(7737..8387)
                     /gene="hyfE"
                     /locus_tag="OO544_002148"
     CDS             complement(7737..8387)
                     /gene="hyfE"
                     /locus_tag="OO544_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HCR6767738.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            complement(8399..9751)
                     /gene="hyfD"
                     /locus_tag="OO544_002149"
     CDS             complement(8399..9751)
                     /gene="hyfD"
                     /locus_tag="OO544_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005136530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
                     /protein_id="HCR6767739.1"
                     /translation="MGVLFAALTTLCMLSLISAFYQADKVAVTLTLVNVGDVALFGLV
                     IDRMSTLILFVVVFLGLLVTIYSTGYLTDKNREHPHNGTNRYYAFLLVFIGAMAGLVL
                     SSTLLGQLLFFEITGGCSWALISYYQSDKAQLSALKALLITHIGSLGLYLAAATLFLQ
                     TGTFALSAMSELHGDARYLVYGGILFAAWGKSAQLPMQAWLPDAMEAPTPISAYLHAA
                     SMVKVGVYIFARAIIDGGNIPHVIGGVGMVMALVTILYGFLMYLPQQDMKRLLAWSTI
                     TQLGWMFFGLSLSIFGSRLALEGSIAYIVNHAFAKSLFFLVAGALSYSCGTRLLPRLR
                     GVLHTLPLPSVGFCVAALAITGVPPFNGFFSKFPLFAVGFALSVEYWILLPAMILLMI
                     ESVASFAWFIRWFGRVVPGKPSEAVADAAPLPGSMRLVLIVLIVMSLISSVIAATWLQ
                     "
     gene            complement(9727..9837)
                     /locus_tag="OO544_002150"
     CDS             complement(9727..9837)
                     /locus_tag="OO544_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoin racemase"
                     /protein_id="HCR6767740.1"
                     /translation="MENLALTTLMLPFIGALVVSFSPQRRAAEWGFCSPH"
     gene            complement(9854..10801)
                     /gene="hyfC"
                     /locus_tag="OO544_002151"
     CDS             complement(9854..10801)
                     /gene="hyfC"
                     /locus_tag="OO544_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HCR6767741.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEDAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GALITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLATGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            complement(10812..12830)
                     /gene="hyfB"
                     /locus_tag="OO544_002152"
     CDS             complement(10812..12830)
                     /gene="hyfB"
                     /locus_tag="OO544_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HCR6767742.1"
                     /translation="MDALQLLTWSLILYLFASLASLFLLGLDRLAIKLSGITSLVGGV
                     IGIISGITQLHAGVTLVARFATPFEFADLTLRMDSLSAFMVLVISLLVVVCSLYSLTY
                     MREYEGKGAAAMGFFMNIFIASMVALLVMDNAFWFIVLFEMMSLSSWFLVIARQDKTS
                     INAGMLYFFIAHAGSVLIMIAFLLMGRESGSLDFASFRTLSLSPGLASAVFLLAFFGF
                     GAKAGMMPLHSWLPRAHPAAPSHASALMSGVMVKIGIFGILKVAMDLLAQTGLPLWWG
                     ILVMAIGAISALLGVLYALAEQDIKRLLAWSTVENVGIILLAVGVAMVGLSLHDPLLT
                     VVGLLGALFHLLNHALFKGLLFLGAGAIISRLHTHDMEKMGALAKRMPWTAAACLIGC
                     LAISAIPPLNGFISEWYTWQSLFSLSRVEAVALQLAGPIAMVMLAVTGGLAVMCFVKM
                     YGITFCGAPRSTHAEEAQEVPNTMIVAMLLLAALCVLIALSASWLAPKIMHIAHAFTN
                     TPPVTVASGIALVPGTFHTRVTPSLLLLLLLAMPLLPGLYWLWCRSRRAAFRRTGDAW
                     ACGYGWENAMAPSGNGVMQPLRVVFSALFRLRQQLDPTLRLNKGLAHVTARAQSTEPF
                     WDERVIRPIVSATQRLAKEIQHLQSGDFRLYCLYVVAALVVLLIAIAV"
     gene            complement(12830..13447)
                     /gene="hyfA"
                     /locus_tag="OO544_002153"
     CDS             complement(12830..13447)
                     /gene="hyfA"
                     /locus_tag="OO544_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HCR6767743.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     LTAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAPAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGEDPLSLTQEQR"
     gene            complement(13700..14170)
                     /gene="bcp"
                     /locus_tag="OO544_002154"
     CDS             complement(13700..14170)
                     /gene="bcp"
                     /locus_tag="OO544_002154"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HCR6767744.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(14170..14742)
                     /locus_tag="OO544_002155"
     CDS             complement(14170..14742)
                     /locus_tag="OO544_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HCR6767745.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            14888..15766
                     /gene="dapA"
                     /locus_tag="OO544_002156"
     CDS             14888..15766
                     /gene="dapA"
                     /locus_tag="OO544_002156"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HCR6767746.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            15783..16817
                     /gene="bamC"
                     /locus_tag="OO544_002157"
     CDS             15783..16817
                     /gene="bamC"
                     /locus_tag="OO544_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HCR6767747.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            17030..17743
                     /gene="purC"
                     /locus_tag="OO544_002158"
     CDS             17030..17743
                     /gene="purC"
                     /locus_tag="OO544_002158"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HCR6767748.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMLNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            17911..18759
                     /locus_tag="OO544_002159"
     CDS             17911..18759
                     /locus_tag="OO544_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HCR6767749.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHNMQ
                     QQGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGDPAQCN
                     TFGKSI"
     gene            18774..>19922
                     /gene="tmcA"
                     /locus_tag="OO544_002160"
     CDS             18774..>19922
                     /gene="tmcA"
                     /locus_tag="OO544_002160"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HCR6767750.1"
                     /translation="MAELTALHTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFNDENFTHTPQGNIVISAFEQTLWRSDPETPLKVYQLLSGAHYRTSPL"
BASE COUNT         5055 a         5325 c         5323 g         4219 t
ORIGIN      
        1 aatggcgaat ggcaatggcg aacggaatca caaacaaatt ggcgatacag tgctcaaacc
       61 cactggcgac aaacagggtg atgggcaaaa tcatggcgac gattttgtcg cataatgaac
      121 gcccgcaata actcatccac agcgccagac aaaccatcag attgcacata atgccgaggc
      181 tgacagattc agtaaatgta tgatgcattt tgccctcggc gcagtgtaaa accgcgactc
      241 cccacatcgc gttttcactc atcaccagcc cggaaaacca gattaacaaa ctgaaacagg
      301 caatacctgc cagattaccg caggccacca gaagtgcgtt aatcagccaa gttcgccaac
      361 taataacgcc ccgacttttt gccatcaccg tcattaccga cgaggtgaac aggctggtgc
      421 cacaaaccgc cagcaaaata aagccgagtg taaagcaaag gccgcccacc agatgggtta
      481 atgcctgtga agacggggca tcggcgataa cggaaaggta aaaagtaaat ccaatcgcca
      541 taaacacgcc agcggacatc gccattaaaa agaccgacac tttgctgcgg gcaattttat
      601 gggcagtaat ccgttcagcg attgccgctt ttctggcgct caactgcaag tcgaaagaga
      661 gtttgtttct catgcatact cctgcaaaag cagattacaa cacctcgcga accgagagcc
      721 ccagacgctg cattcgtgac agcagcgtgg tgcgcttcat ccctaatcgt gtcgccgcgc
      781 cacggggccc ggcaacaatg ccattggttt ctcgcaatac ctgaacaatg cgctgacgtt
      841 cttcttcgtc attctctggc gtcgtcgggt gcagcaactg cgccattgaa ctgcgaagcg
      901 ctgaatcttc atttagcgtc ggcagtaaat ggctttgtcg gacattaaga tgtaaattca
      961 gactgttacc gcgagtcagc agtaccgctc gctcaatcac gttttccagc tcgcgcacgt
     1021 tgcccggcca gtcccacgac atcaactggc gtagcgcctc ggtcgggatg gcgtcaattg
     1081 aacgattcat atggcgcgcc attttttgcg tgaaatgctt tgctaaaaga gggatatctt
     1141 ccggacggtc gcgtagcggc ggcaattcca gtgggaagac attcaggcga taaaagagat
     1201 cgctgcgaaa ctggcgatct tcaaccattt gccacaaatc acggttggtg gcggcaatga
     1261 ctctgacatt caccgggatc gttctactcc cgccgagacg ctcaatctcc cgctcctgca
     1321 atacgcgcag cagtttaggc tgaagttcta acggcagatc gccaatttca tcaagaaaca
     1381 acgtgccgcc atcggcaatt tcaaaacgac cacgatgggt attaatcgca ccagtaaacg
     1441 cccctttgtc atgaccgaat aactcacttt ccagaagact ggcggggatg gcagcgcagt
     1501 tgattttgac cagcggcttg tcgcgtcgcg ggctaagttg atggatcgct ctggcgatca
     1561 cctctttgcc ggttccggtt tcaccacaaa tcaacactgt actgtcgctc ttcgccacaa
     1621 tatctacctg ctggagcagg tcttccattg cctggctttg atagataatg tcaccgacgc
     1681 ccagattcga aaggagctgc tcgctaagct ggtggttttc ttgctgcaaa ctttcctgca
     1741 aatcggtcat gctacgccag gcatcggcat tgccaacggc aatagcgatg cgatcggcta
     1801 tgtgttgtag tagctgacag ttttcctcac taaagagagt ggaagaggta tgcgccagca
     1861 acaatgcacc cggtgtatgg ttgccaaagg taagcggtat aaggagcgca gattcgcagc
     1921 cgttagatgc aagtaagagt aataacgggt cgcgttgcca gagaaacaga tcgtctgcac
     1981 ggtgcgtcaa ggtcggttgt cgggtttgta gcgtttgtgt cagcaataca ctgtcgccag
     2041 gcatattgcg tggcagacac gcacaatgtg aggcagaaag atcgctgcac cacaggctaa
     2101 acttctcgtt ctttcgatga tcgcccagta ccatactgac tgaagccaga ccgaaaaaat
     2161 gatggatctc acgagcgacg tcagcgatca gatcgtcgag atcaagatgt gacagcactg
     2221 cattagtaat atcaaccagt acccgatgat gatcgcgatc tttctccagc aaacgcagcg
     2281 ccacctgctg ttcttcatca ttgagccatg attgcagaac cggggtgatt atccgcgcca
     2341 gccagttaaa cagtgtgcaa tcctggggag taaaacggca gccgtgggtg cgaataaagc
     2401 gtagctccgc cattccacta ctgccgagaa ggacggcgtg acgcgaataa tcgaccatct
     2461 cgccctgata ctctgttgtt gccgtcacat cgctgccatc atcatggtag cggtaataga
     2521 agttgctttt gagaacatgc agttcaataa gttgtaccgg gctgaacggc ggcggcagcg
     2581 ggatgaaggc gcgtaataaa gacgccttac cgtgtttctg cgctaaccgc tccgcgagca
     2641 ttccgtttac cgcttcaatt gttattcctt gcggcggggc aaacatcgcc tcgtctgaca
     2701 tagccatcaa cagtccttat taatgagaaa tccttctcac gcaatttttc tggccatcag
     2761 atagatggcc ggttcctgca caatttcatc caacagatga atgaattgaa tgctacgcgc
     2821 ctgccacaca gacgtcgcgt catccgccag tggcgcgaat gcccgggtca acagggcggt
     2881 gtggctggcg tcaataacaa tctcaccaaa agtcatcacc cccagcatct tacgtcgggc
     2941 ttgctcctct tcgaccagtg caagccaatc ttcgtattcc gtcagtgggc aggggaaggc
     3001 gacattcaga caatcaatca cgccaacgtg atgaccgata gccagcgagt aatacattac
     3061 ctgagagctg tgttccggca tctcgtcgct actggcgaca aacttttttc gcagcgtcca
     3121 gaaaacaatt tccccgcact cttcagtcat gtaacccccg gagtacgcga aagagttgct
     3181 gaacgatact attgagacgt ggatcgtcgg catcgcgcaa ccaggtgtta acccgatttc
     3241 ctgtaggatc gtcgcttaaa tggcgcagga gccgatcgca aatttctcgc ccctgacgat
     3301 agcccgccag ccgccgcgct tctcgctcaa tggcgatacg ctgtgatggc gggatctgcg
     3361 gccacaacgg ttgcatagtc accccggtgg gatcgcgata atccaccgcg tgaatcttct
     3421 gttgcagcaa accgagcgcc acggcgaaac cgtgaatggt ggcggccggt gttggcgggc
     3481 agccggggat ccaaacatca atggggacaa tggtgtcgct gccgccccag acgctgtaga
     3541 gatcgtggaa aataccgccg ccgacaccgc acgcgccgta ggaaacacaa attttatgat
     3601 cgggggcaga ctcatacgcc cgaagtgcag gcatacgcat cgcccgggtg actgcgcctg
     3661 taaataacaa aatatcggcg tgacgcggtg atgaaataac cttaatgcca aaacgttctg
     3721 cgtcgaatac tggtgtgatg gcggcaaaaa tttcgatttc acaggcgttg cagccgccgc
     3781 aatcgacgcg ataaacgtaa gccgagcgac ggatatcctg tagcaaatgc tgcttcatct
     3841 tttgcgtttg ctcgtccagc gtgatgggct ggctgacatg ttgtgtaagc actggactca
     3901 tagctgctcc ttagccacca gcggtacatc tgtatcgtcg ttgagcagcg tcgcgcgttg
     3961 tttgcactcc gggcagacgc tcgcttgcgc ccataacatt tcgcggtttt gtggcgcatt
     4021 ttgttgctgt gccaacaatt cagcagccag tgcgacggtt ttttgcgggg caaacgggcg
     4081 ttcgcaacgg ctgcaacgtt gtagatggaa cgtcgcgcgg gtatagagat cggctttatt
     4141 ggtgacggtc agttcaaagt tattggtaag ctggatggct ctggtcgggc acacttcttc
     4201 acaacgtccg cagtaaatac aacgcctcag atagagctgc caggtgcgcg aattttgctg
     4261 gtcgtcggtc tggatagtca gcgcatttgc cggacaagca caggcgccgc aggcaataca
     4321 ttggctgggc atcaggtccg gtttcccgcg aaagcctggg ctgacctcca gtggcgcgaa
     4381 gggatatttc accgtcgcgg ttccggcgcg cataatagtt ttcagtaact tcagcatctt
     4441 ctgtccttac ttcagcggcg aacggttacg atcaatgccg tagcgttcga tctctttata
     4501 cggcacggtt tttgactggc gcttgcgcac atctaccagc gtcacacggt cggtacagga
     4561 gtagcaggga tcaaggctac cgataatcag cggtgcgtca gaaacggtat tgccgcgcag
     4621 catgtaacgc aacaccggcc agttggcgta ggtggcggca cggcagcgcc agcggaacaa
     4681 tttttggtta tcaccgagca tgctccagtg cacgtcttca ccgcgtggcg cttcaacaaa
     4741 gcccagcgcg aatgcgtgag gtttatagct aaagccttcg gtcagcagtg gggtatccgg
     4801 catgttgtcg agggcaaatt ccagcattgc cagcgaatca aacgtctctt tgacacggac
     4861 catcacgcgg gagaaaacat cgccgccggt aaaggtaaac agtgtttttg gaatattgcc
     4921 gtagtcggcg taggggtgat caaagcgcaa atcacgggca aaaccactgc cgcggatcag
     4981 cggccctaca gggctataat cacgggcgat ttgtcggtcg agaatgccaa tgccctgagt
     5041 gcgttgttcc atattcggcg tggcaagcag catctctacc agctccgaca cgtcggcgcg
     5101 catctcgcgc accagtttca gcgtttgcag acgttgctct ttgagaatat cgcggcgaac
     5161 accaccaatc agattcagac cgtaggtttt acgcgacccg gtcagcaatt ccgccattgt
     5221 catcgacttt tcccgcacgc ggaaaaattg cataaagccg gtatcaaatc caacaaaatg
     5281 gcaggagagg ccaaggttga gcaaatgact gtgcagacgt tcgacttcca gcagaatcga
     5341 gcgaatagta tgcgctcgtt gcggcacctc aatccccagt gcattttcaa ccgagttggt
     5401 ataggccaca ctgtgggcaa aaccgcaaat cccacacacg cgatccgata agaaggtcac
     5461 ttcgttgtag cccatccgcg tttctgccag tttctccatg ccgcgatgga cataaaacag
     5521 gcggtaatca gcatcgacaa tttgctcgcc atccacaaac aagcggaagt gacccggttc
     5581 atcggaggtg atatgcagcg ggccaatagg gatcacccgc gcatcgctat tgccctcatt
     5641 gataaacgga tacgtttcgg tatcagtcgt cggttcaggg cgcagtcgat aatccatcgc
     5701 atctttgcgc agcggatgca tatcttccgg ccagttatcg ggcaacacca ggcgacgctg
     5761 atccggcagg ccaaccggaa tcagcccgta catatcgcga atttctcgct cgccccagac
     5821 cgcggcaggg acgcgcggtg tgactgacgg aaactcccga ctgtcggcat cgaccagtgc
     5881 cttcaccacg atccagcatt tttcggcccc ttccattgaa agggcataat aaaccgcgta
     5941 atgaccgtta agtgtccgct cgtcgttgcc aaacaggact ggaagccagc catcatgttg
     6001 ataataaaga taatgtacaa cgtcaggcag cagattgatt ttcaccgtaa tggtgacctg
     6061 ttcaggcgtt tgtcgctctt catccagcac cgcgccgggg aactgcgttt tcagggcggc
     6121 gagaatcgct tcgccacgat ttgacgatga attaacgttc actgtgtttc tccgaaacag
     6181 atgcggtgcc tgaaggcaaa aaatcatgcc aggtgctacg ttgtgcaggc aggtcgtgcg
     6241 tccctgagag gactatagtg gaagcgcccg ccaggatcct gatgacaggt tgtggaatat
     6301 gcgttcccat cgccagcatc atgaccagca gaatcaccat tggcgaggtg gtcaaccagc
     6361 cgagatcacc ccggttaacg gcctgcggcg gtttcgccat taacacccgc gctgccatcc
     6421 gtaccaggcc cgccagcacc agcgttaaca gcaataacag caggacgata atcagcaggt
     6481 gattacgtgc cagtccggcg gtaatggtca taaattcgct aagaaaaatg ttgaagggcg
     6541 gcatccctgc cagcgccagc gcaccgccgc caaacagcac ggcggtaaat ggcatgattt
     6601 tgagcatccc acagacgacg ttgagatcgc gcgtgccgta cttgagcagt acattgccgg
     6661 aaccgcagaa cagcagcgtt tttgccagac tgtggtttaa gatgtgcagc agcgcggcaa
     6721 aaattcccag cggcccgcca atgcctagcg ccaccgcgac cagccccatg ttctccacgc
     6781 tggagtacgc cagcagacgc ttaatgtccc gctgtaccag aatgaaaaat gccgccacgg
     6841 caaccgacaa catgccgaag atgagcaaca accggttggg gaaatcgctg ccgatggctt
     6901 ggcaaataat gatgtaatag cgaatcagca ccaacagcgc gcagttcagc aatacggcgg
     6961 agagcagggc gctgaccgga ctcggcgctt cactgtgagc atccggcagc caggcgtgca
     7021 tgggaaatag cccggttttg gtaccaaagc caattagcac aaacacaaag gccaacagca
     7081 ttaatgttgg gtcaagcaag gacgattgct taagaacctc gctccagaat atcgccattt
     7141 ctgcctgcgg cataacgctg gcggcgttgg cgtataccag cacggtaccg aacagaccaa
     7201 aagcgacacc aacagtacaa ataatgatgt acttccatgc tgattccagc gatgaacgct
     7261 gaccgtaaat gcctaccaga aacgccgagc ttaaggtggt ggcttcgatc gccgcccaca
     7321 tcacaatcag gttattgctg gtaacaacca gcagcatggt gaacaaaaac agatggaaga
     7381 aaccgtagta atcgcacagc gtaacgggtg aaagctcgcc gtgtgccact tcatgacgca
     7441 tgtagccaat cgagtaaaca ccggtgagaa agccaatcac accaagaatg gcgaggaaca
     7501 aaccgcccag accatcaata tgaagccaca gtcccgcagc gaatatttct cctgcatcag
     7561 cggcagtttg gaccacccag agtgccagaa tcagcagcag tgtgatccct aagctatgta
     7621 atacagtcac cgtacaagtc gcagagagcc cccgtttccg gcaggcaaaa cagagcagcg
     7681 aaaaaagcag cggcgtgagc aggagtaaag cgaacatcac agaataactc atctcattat
     7741 cccttcagcg cggtcaagtt gttcacgtcc agcgtgccgt gggtacgcca tatttttctt
     7801 gccagtaaca ccatcacaat gacggcgaag atggcgtcgg tagcgatacc tatttccacc
     7861 agttccggtg ctcgccaggc aagaagcgcc agcaccagat gggagccgtt ttccatcagg
     7921 cagtaaccaa aaatttgccg caggatattg cgctggctga caatgcacag caggccaagc
     7981 agaaaatgac ctaacgctac cgccagcgcc ggtttcagcc cggtagccat cggtagcttc
     8041 acgggctgaa cgacaaatgc gcaaagcagg acaattaacg ccgcgagcag tgccatcatt
     8101 gccggaccga ataacgcttt ttccgggatg ttctggggaa tatttcgtgc agcgtaagtc
     8161 atgattaacg gtaccagcag cactttggtg ataaaggcgc tggcggacca gatcagcagt
     8221 tgctctgcgg cgaacaggca cgagagagtg gcgaaaatag acaccagcac cagcgactgg
     8281 caggcgtaaa atccgcatga caggcgatag cttttgacgc taatcacaaa cagcgatgtc
     8341 agcatcatca gtcccgccag attatttacg atcatagaac cggtcattca tcatctcctt
     8401 actgcaacca ggtcgcggcg attacgctgg aaatcagcga catcacaatc agtacaatca
     8461 acaccaggcg cattgaccct ggcagcggtg cggcatcggc gacggcctcg ctcggtttgc
     8521 ccggcacgac gcgaccaaac cagcgaataa accaggcgaa actggcgacc gattcaatca
     8581 tcagcagaat catggcgggc agcaggatcc agtactccac tgacaacgca aaaccgacgg
     8641 caaacagcgg gaatttactg aagaagccgt tgaacggcgg cacaccggta atcgccagcg
     8701 ctgccacgca gaaacccacg cttggcaacg gcagggtgtg caatacgcca cgcagacgcg
     8761 gcaacaagcg cgtgccgcag ctgtaactca gcgcacctgc tacaaggaaa aacaggcttt
     8821 tagcgaacgc gtggttgacg atgtaggcga tgctaccctc cagcgccagc cgcgagccga
     8881 agatggagag cgacaagccg aagaacatcc agccaagttg agtgatggtc gaccaggcca
     8941 gcaaccgctt catatcctgc tgtggcaaat acatcagaaa gccataaaga atggtgacca
     9001 gcgccatgac catgccaacg ccgccaatca catgcgggat attgccgccg tcgataatgg
     9061 cgcgggcaaa aatgtaaacg cccactttca ccatcgatgc ggcgtggaga taggcactga
     9121 tcggtgttgg cgcttccatt gcatccggta gccacgcttg catcggtagc tgggccgatt
     9181 tcccccacgc ggcaaacagg atgccgccat aaaccagata acgtgcgtcg ccgtgtaact
     9241 cgctcatcgc gctaagcgca aacgttccgg tctgcaaaaa cagcgtggcg gcggcaagat
     9301 acaaccccaa cgagccgata tgagtgataa gtaacgcttt tagtgctgaa agctgtgctt
     9361 tatcgctctg gtaataactg atcaacgccc aggagcagcc gcccgtaatt tcaaaaaaca
     9421 acaactgacc gagcagcgtc gaggagagta ccagtcccgc catcgcgccg ataaacacca
     9481 gcaaaaatgc gtaataacga ttcgtgccgt tatgcgggtg ttcgcgattt ttatccgtca
     9541 gataacccgt ggagtagatc gtgaccagca aaccgagaaa caccaccaca aacagaatca
     9601 gcgtactcat gcgatcaatg accaagccaa acaatgccac atcccccacg ttgaccaacg
     9661 tcaacgtgac ggcaacttta tcggcctgat aaaacgcgga gatcagcgac aacatgcaca
     9721 gcgtggtcag tgcggcgaac aaaaccccca ttcggcggcc cgacgttgtg gcgaaaacga
     9781 aacgaccagt gcgccgataa aaggcagcat taacgtcgtc agagcaagat tttccatatt
     9841 ccgtcagtgc tccttacaga ccggttaacc agaagaccca tgcaagcgca gcaaggctga
     9901 agccaagcca ggtcacatgg tgaatgagta aaaaacgccc gcgtgccagc gtgttttctg
     9961 cgattgaggc cagtacaaaa atcagcaaaa ccttgagcag cgtaacgaca agtgaagtca
    10021 gcaggcaggc gagagaaagt tcttgcgcgc gcccaaaggg cagaaacagg gccacaaaca
    10081 gtgctgccat cacgacctgt ttcagcccca gcccccattt cgctagcgcc agcccggcac
    10141 cggaatattc ggtcagcggg ccttcctgta attcctgttc tgcttcagca acatcaaagg
    10201 gaatttttcc catctcaatg aagcaggcaa aaccacaggc cagtaacgcc agtacggtgg
    10261 ttagcggcga gttccagccc gttgccagcg tattgctgat catctcgata tgcgtggaac
    10321 ctgctatcag cgccaatacc agcagtgaga gaatgagcat gggttcgacc agaatgccga
    10381 gcgtcaactc gcgactggca ccgactcccg caaacgggct tccggtatcc agccctgaaa
    10441 gagcaaagaa aaaacgaaac agggcaagaa gatagataag ggtgatcaga gcgccgccgc
    10501 ccgcaaaagg ggaaacggta ataaatagtg gtagggccat cgccagcacc agcatgctgc
    10561 tgattaatac ccacggcatc aggcggaaca tcagacctga agatgtcggc gcatcttcct
    10621 ggcgtttaaa cagtttgtgg atatcgcgat aatcctgcca gatccccggc ccgcggcggg
    10681 agtgcatacg cgcgcgtatc tgccgggaaa tacccgtaaa aagtggggtt agcatcagca
    10741 gaatcacggc ctgtgctaac gcaaaaatga ccagatatcc gtcgcaaaga gtttgtctca
    10801 tggtgatttc cttagacggc aatagcgatt agcagcacaa ccagtgcggc gaccacatac
    10861 aggcaataga gacgaaagtc gccgctttgc agatgctgta tttctttggc cagccgttgg
    10921 gtggcgctca cgatggggcg gatcacccgc tcatcccaga agggttctgt gctctgagcc
    10981 ctggcggtga cgtgcgcaag acctttattt agcctcagcg tagggtcgag ctgttgtcgt
    11041 agacgaaata gcgcagaaaa gaccacacgc agcggctgca tcacgccatt gcctgacggg
    11101 gccatcgcat tttcccagcc gtagccgcat gcccaggcat ctcctgtgcg acgaaacgct
    11161 gcgcggcgcg aacgacacca cagccagtaa aggccaggca gcaaaggcat cgccagtagt
    11221 aacagcaaca gtaatgaagg agtgacccgt gtatgaaacg tgccgggtac aagtgctatt
    11281 ccgctggcga cagtgacggg aggggtattg gtaaacgcat gggcaatgtg cattatcttc
    11341 ggtgccagcc aactggcact aagcgcaatt aatacgcaga gtgccgcgag cagtagcatg
    11401 gcgacgatca tcgtatttgg cacttcctgt gcctcttcag cgtgtgtgct gcgcggcgca
    11461 ccacagaaag taataccgta cattttgacg aagcacatta ctgccagccc accagtgact
    11521 gccagcatta ccatagcaat aggacccgca agttgtagcg ctacggcttc cacacgactt
    11581 agtgagaata gcgactgcca ggtgtaccat tcgctgataa aaccattcag cggaggaatg
    11641 gctgatatcg ccaggcaacc aatcaggcat gctgcggctg tccacggcat ccgtttcgcc
    11701 agtgccccca ttttttccat gtcgtgggta tgcaaacgcg aaataatcgc acccgcgccg
    11761 agaaatagca gccctttgaa aagcgcatgg ttgagcagat gaaacagtgc gccgagcagt
    11821 ccaaccacgg tgagcagcgg gtcgtgcagt gacagaccga ccatcgccac accgaccgcc
    11881 agcaaaataa tgccgacgtt ttcgacggta ctccaggcca gcagccgttt gatatcctgt
    11941 tccgccagtg catacagcac gcccaggagc gcggagattg cgccgatcgc catcaccaga
    12001 atgccccacc acagcggcaa acccgtttgc gccagcagat ccatcgccac tttcaggatg
    12061 ccgaaaatac cgattttgac cattacgcca gacatcaacg ctgaagcgtg cgatggtgcg
    12121 gcagggtgag cgcgcggcaa ccagctgtgc aacggcatca tcccggcttt cgcgccaaaa
    12181 ccgaaaaagg ccagcaggaa caccgccgac gccagccccg gagaaagtga aagcgtgcgg
    12241 aaactggcaa aatcgaggct gccgctttcg cgccccatca gcaagaaggc gatcattatc
    12301 agcaccgatc cggcgtgggc gataaaaaag tagagcatgc cagcgttgat cgacgtttta
    12361 tcctgcctgg caatgaccag aaaccaggaa gacagcgaca tcatttcaaa cagcacgatg
    12421 aaccaaaaag cgttgtccat caccagcagg gcaaccatcg atgcgatgaa aatattcata
    12481 aagaagccca tcgccgccgc gcctttgccc tcgtattcgc gcatataagt caatgaatag
    12541 agagaacaaa ccaccaccag caaggagata accagcacca taaatgccga gaggctatcc
    12601 attcgcaggg ttaaatcggc aaattcaaaa ggcgtggcaa aacgggcgac taaagttacg
    12661 cctgcatgta attgcgtaat tccgctgatg atgccaatca cgccgcccac cagcgatgtg
    12721 atgccggaaa gcttaatagc cagtctgtcc agaccgagta aaaacagcga agccagactg
    12781 gcaaacagat agaggatcag cgaccaggtt aataattgca gggcatccat tagcgttgct
    12841 cctgagtgag ggaaagggga tcctccccgc cattggcaaa ccagcttgcg gcaaggcgct
    12901 gtttttcttt catctggcgt tgcaggctat cgtcggtgat cagccgtaac gcctgattcg
    12961 ggcaagcgcg aacgcaggcc ggaccttctg gcaagaaatc acacaggtcg catttcaccg
    13021 cgacggtctg gacacccggt tcccagcgca gcaacgcatg ttggggcggc acgttttctt
    13081 cgccgtcttt gagtgagccc tcagcctgaa aaacatattg cgcaggggca ttcaccggac
    13141 ggcttcctga agcgctgatt gcgccaaatg ggcagaccac ggcgcaaagc ttgcagccaa
    13201 tacagaggct ttcgttgagt tggatcgcat catccctctg agagatggca ttgaccgggc
    13261 agacctgcag gcaaggggct tcctcacagt gatgacacac gacaggggca gtgagtgttg
    13321 acgtcttcgc cagggccagg cgcgggtgtt gctgtaaacc ttgcgtttta tgcacgtccg
    13381 aacaggcagc gagacaggta ttacatcctg tacaccacag tggttcggcc accacaaagc
    13441 ggttcatgtc ggctccttga aaatgatatg aaacattgca tttcatattg cataaaggat
    13501 gctattctga ttaactcaat atattcaata agttatgtat taactggatt ctgaacggta
    13561 caaaaatgat ggtcattaac gttcagcaaa aaatggattc gacacccgtg tcgcagggga
    13621 aagcgagaat gtgatcttga tcacgagaag tgaggtatgc tggccgcaag cgcagccagc
    13681 acggaatgga gcaaagtaat caggcgtgtt ctttcagcca gttcagcaca acgtcgtggt
    13741 gattgctggt tttgaaatcg tcaaagacat gttcgatttt gccatcagcg tcaatcagga
    13801 agctgatgcg atgaatgcca tcgtaggttt tgcccatgaa ggacttttca ccccagacgc
    13861 cgaattgttc gcacacctgg tggtcctcat cagacaggag cgtaaagtta agcagctctt
    13921 tttccgcaaa acgggagagt ttttcgggtt tatcggtgct gatacccagc acatcaacgc
    13981 ccgctttttt caactcatcc atgttatcgc gtaagccgca ggcctgtacg gtacagccgg
    14041 gggtcatggc tttcgggtag aaataaacca gaacacgctg tccctggaag tcggtcaaat
    14101 taacttgttc tccgtcttga tccggcaagc taaatttcgg tgcgatatca ccggctttca
    14161 gtggattcat tacttaactc catcctgttc atcatgttgg gaataattga cgacgttaat
    14221 actgccttgt gcattgagtt ctgtacatag ggctttgaac gcttgctcaa tatttgctgc
    14281 gtccgcagat gcggggctgt gggcggttat ctgaatatgc aactgcgcag cccgttcatt
    14341 ttcagcaggt tgcgtgcgcg acaccagctc cgcaatgttc atatgatgcg cgtcgaaaag
    14401 tgctgtgaag cgttcaatta aatgcgggga gtctgccaca tcgacctgaa cccagacaga
    14461 tgctggcatt ggcggacgcg gacgcgccgt cgtgcgcttc atcacgatta aaagatccag
    14521 ctcggcacct ttcaacggta acgttgattc aatcagagta atggcattcc atgaaccgga
    14581 aagcagcatg ataaacgtga actcttctcc cagcatcgcc aggcgactgt cttcaatatt
    14641 acagccgcaa ctactgacat gacgggtgat ggtgttcaca attccagggc gatcggcacc
    14701 caatgcagtg atcaccagat aatgttgcga tgacagtgtc aaactggtta ttcctttaag
    14761 gggtgagttg ttcttaagga aagcataaaa aaaacatgca tacaacaatc agaacggttc
    14821 tgtctgcttg cttttaatgc cataccaaac gtaccattga gacacttgtt tgcacagagg
    14881 atggcccatg ttcacgggaa gtattgtcgc gattgttact ccgatggatg aaaaaggtaa
    14941 tgtctgtcgg gctagcttga aaaaactgat tgattatcat gtcgccagcg gtacttcggc
    15001 gatcgtttct gttggcacca ctggcgagtc cgctacctta aatcatgacg aacatgctga
    15061 tgtggtgatg atgacgctgg agctggctga tgggcgcatt ccggtaattg ccgggaccgg
    15121 cgctaacgct actgcggaag ccattagcct gacgcagcgc ttcaatgaca gtggtatcgt
    15181 cggctgcctg acggtaaccc cttactacaa tcgtccgtcg caagaaggtt tgtatcagca
    15241 tttcaaagcc atcgctgagc atactgacct gccgcaaatt ctgtataatg tgccgtcccg
    15301 tactggctgc gatctgctcc cggaaacggt gggccgtctg gcgaaagtaa aaaatattat
    15361 cggaatcaaa gaggcaacag ggaacttaac gcgtgtaaac cagatcaaag agctggtttc
    15421 agatgatttt gttctgctga gcggcgatga tgcgagcgcg ctggacttca tgcaattagg
    15481 cggtcatggg gttatttccg ttacggctaa cgtcgcagcg cgtgatatgg cccagatgtg
    15541 caaactggca gcagaagggc attttgccga ggcacgcgtt attaatcagc gtctgatgcc
    15601 attacacaac aaactatttg tcgaacccaa tccaatcccg gtgaaatggg catgtaagga
    15661 actgggtctt gtggcgaccg atacgctgcg cctgccaatg acaccaatca ccgacagtgg
    15721 tcgtgagacg gtcagagcgg cgcttaagca tgccggtttg ctgtaaagtt tagggagatt
    15781 tgatggctta ctctgttcaa aagtcgcgcc tggcaaaggt tgcgggtgtt tcgcttgttt
    15841 tattactcgc tgcctgtagt tctgactcac gctataagcg tcaggtcagt ggtgatgaag
    15901 cctacctgga agcggcaccg cttgcggagc ttcatgcccc ggctggaatg attttgccgg
    15961 tgacctccgg tgattatgca atcccggtga ccaacggtag tggtgctgtc ggtaaggcgc
    16021 tggacattcg tccaccagcc cagccgctgg cactggtttc tggcgcgcgt acccagttca
    16081 cgggcgatac cgcttcattg ctggtggaaa atggtcgtgg caatactctg tggccgcagg
    16141 tggttagcgt gctgcaggcg aaaaactaca ccatcaccca acgtgatgat gctggtcaga
    16201 cactgaccac cgattgggta caatggaacc gtctggacga agacgagcag tatcgtggtc
    16261 gttatcaaat ctctgttaag ccgcagggtt atcagcaggc ggttacggtt aaactgttga
    16321 acctggaaca ggcgggcaaa ccggttgcag acgcggcttc catgcagcgt tacagcacgg
    16381 agatgatgaa cgttatttcc gccggtctgg ataaatctgc cactgacgcc gcgaacgctg
    16441 cgcaaaatcg tgcctccacc actatggacg tacaaagtgc agctgatgac accggtttac
    16501 caatgctggt cgtacgcggg ccgttcaatg tggtttggca acgtctgcca gcggcgctgg
    16561 aaaaagtggg catgaaagtg accgacagca cccgttcgca gggcaacatg gccgtaactt
    16621 ataagccgct gtctgacagc gactggcagg aactgggcgc gagcgatcca ggcctggcat
    16681 ccggtgacta taaactgcag gttggcgatt tagataaccg cagcagccta cagttcatcg
    16741 atccgaaagg tcatactctg actcagagtc agaacgacgc gctggtagct gtcttccagg
    16801 ctgcgtttag caagtaaaaa tacagggctg gaatcatccg gccctttttt ctgatatgat
    16861 acgcaaacgt gtgcgtcggc aggaaaacgc gattttagcg gtaattcgca cgaaatttgt
    16921 ttgtcggacg tagttcggat aaggcgttca cgccgcatcc gacaaaacat ccggcacacc
    16981 agacagcaaa agattttaaa acgttaattc acacccagga gtgataaaga tgcaaaagca
    17041 agctgagttg tatcgtggta aagcgaaaac cgtatacagc acggaaaacc cggacctgtt
    17101 ggtgctcgaa ttccgcaatg atacgtcagc aggggatggc gcgcgcattg agcagtttga
    17161 tcgcaaaggt atgttgaaca acaagttcaa ctacttcatt atgagcaaac tggctgaagc
    17221 gggtatcccg actcaaatgg agcgtctgct ctccgatacc gaatgtctgg tgaaaaagct
    17281 ggatatggtg ccggttgagt gtgtcgtgcg taaccgtgct gcgggctctc tggtgaaacg
    17341 tcttggaatc gaagaaggta ttgagctgaa cccgccgctg ttcgatctgt tcctgaaaaa
    17401 cgatgccatg cacgatccga tggtcaacga atcttactgc gaaacctttg gctgggtgag
    17461 caaagagaac ctggcgcgta tgaaagagct gacctacaaa gcgaacgacg tgctgaaaaa
    17521 actgttcgat gatgctggtc tgattctggt cgacttcaag ctggagtttg gtctgtacaa
    17581 aggtgaagtg gtactgggcg atgagttctc cccggacggc agccgcctgt gggacaaaga
    17641 aacactggag aaaatggaca aagaccgttt ccgccagagc ctcggtggcc tgatcgaagc
    17701 ctatgaagcc gtcgcccgcc gcctgggtgt acagctggac tgatttttct gttcatcatc
    17761 ttgccgtgca gctggcacgg caagacaacc gctctcggaa tatatgcatt gaacgctcat
    17821 tcctccgcca tttcatcccg ctttcctgtg gtaatcttcc tctgaaccac ctacgatcat
    17881 ttctataatg aatatattgt tgaggtatct atgcgttggc aagggcgacg tgaaagtgac
    17941 aatgtcgaag acaggcgtaa cagctccggc ggtccgtcaa tgggcggtcc cggttttcgc
    18001 ctgccaagcg gtaaaggcgg gctgatttta ctgatagtcg tgctggttgc aggctactat
    18061 ggtgttgatt taaccgggtt gatgaccgga cagccggttt cccaacagca atcaacacgg
    18121 tcaattagcc caaatgaaga cgaagccgca aaattcacct cggtgattct ggcaaccacg
    18181 gaagacacct ggggacagca attcgagaag atgggtaaaa cctatcagca accgaagctg
    18241 gtcatgtatc gtggaatgac gcgtaccggc tgcggggcgg gccagtccat tatgggaccg
    18301 ttctattgcc cggcggatgg cacggtttat atcgatctct ccttctatga tgacatgaaa
    18361 gacaaactcg gcgcggatgg cgattttgcc caggggtacg ttatcgccca tgaagtcggt
    18421 catcatgtgc agaaattgtt aggcatcgag ccgaaagttc gtcaactgca acaaaacgcg
    18481 acgcaggcgg aagtaaaccg cttatctgtg cgtatggaac tccaggccga ctgttttgcc
    18541 ggtgtctggg ggcataacat gcagcagcaa ggcgatctgg aagaggcgct gaacgcggcg
    18601 caggccatcg gcgatgaccg tttacaacag caaagtcagg ggcgagtagt accagacagt
    18661 ttcactcatg gtacttctca gcaacgctac agctggttta aacgtggttt cgacagcggc
    18721 gatccggcac aatgcaatac ttttggtaaa agcatttaac ttccggggca aggatggctg
    18781 aactgactgc gcttcacaca ttaacagcgc aaatgaaacg tgaagggatc cgccgcttgc
    18841 tggtgttgag cggggaagag ggttggtgtt ttgatcatgc gcttaagttg cgtgatgcct
    18901 tacctggcga ctggctgtgg atttcgccgc agccagatgc tgaaaaccac tgttctccct
    18961 cggcgctaca aactttactt gggcgcgagt tccggcatgc ggtattcgac gcccgccacg
    19021 gctttgatgc cgctgccttt gcggcactta gcggaacgtt gaaagcggga agctggctgg
    19081 ttttgttact ccctgtatgg gaagagtggg aaaaccaacc tgatgccgac tcgctgcgct
    19141 ggagtgattg ccctgaccct attgcgacgc cgcattttgt ccagcatctc aaacgcgtac
    19201 ttacggcgga taacgacgct attctctggc ggcaaaacca gccgttctcg ttggcgcatt
    19261 ttaccccccg tactgactgg caccccgcga ccggcgcacc acaaccagaa caacagcaac
    19321 tcttacagca gctactgacc atgccgccgg gcgtggcagc ggtaacggct gcgcgtgggc
    19381 gcggtaagtc cgcgctggca gggcaactca tttctcgtat tgcgggcagt gcgattgtca
    19441 ccgcgcccgc aaaagcggca acggatgtac tggcacaatt tgcgggcgag aagtttcgct
    19501 ttattgcgcc ggatgccttg ttagccagcg atgagcaagc cgactggctg gtggtcgatg
    19561 aagccgcagc catacctgcg ccgttgttgc atcaactggt atcgcgtttt cctcgaacgt
    19621 tgttaaccac tacggtgcag ggctacgaag gtaccggacg tggttttttg ctgaaatttt
    19681 gcgctcgctt tccgcattta caccgttttg aactgcaaca gccgatccgc tgggcacagg
    19741 gatgcccgct ggaaaaaatg gttagtgagg cactggtttt taacgatgaa aacttcaccc
    19801 atacaccaca aggcaatatc gtcatttccg catttgaaca aacgttatgg cgaagcgatc
    19861 cagaaacgcc gttaaaggtt tatcagttat tgtctggtgc gcactaccgg acctcgccgc
    19921 tg
//
LOCUS       DAJTZL010000071        19669 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.071, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000071 DAJTZL010000000
VERSION     DAJTZL010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19669
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.071"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..494
                     /gene="gstA"
                     /locus_tag="OO544_002161"
     CDS             <1..494
                     /gene="gstA"
                     /locus_tag="OO544_002161"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HCR6767751.1"
                     /translation="NGDDYFAVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQL
                     LAPVNSISRYKTIEWLNYIATELHKGFTPLFRPDTPEEYKPTVRAQLDKKLQYVNEAL
                     KDEHWICGQRFTIADAYLFTVLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAE
                     GLK"
     gene            complement(538..1401)
                     /gene="pdxY"
                     /locus_tag="OO544_002162"
     CDS             complement(538..1401)
                     /gene="pdxY"
                     /locus_tag="OO544_002162"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HCR6767752.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEVLEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(1460..2734)
                     /gene="tyrS"
                     /locus_tag="OO544_002163"
     CDS             complement(1460..2734)
                     /gene="tyrS"
                     /locus_tag="OO544_002163"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HCR6767753.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            complement(2863..3519)
                     /gene="pdxH"
                     /locus_tag="OO544_002164"
     CDS             complement(2863..3519)
                     /gene="pdxH"
                     /locus_tag="OO544_002164"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HCR6767754.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHAQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            complement(3578..3907)
                     /gene="mliC"
                     /locus_tag="OO544_002165"
     CDS             complement(3578..3907)
                     /gene="mliC"
                     /locus_tag="OO544_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HCR6767755.1"
                     /translation="MSMKKLLLICLPVLLTGCSAFNQLVEHMQTATLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGVRYTDGIYVFWSKGDEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            complement(4005..5114)
                     /gene="anmK"
                     /locus_tag="OO544_002166"
     CDS             complement(4005..5114)
                     /gene="anmK"
                     /locus_tag="OO544_002166"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HCR6767756.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANP"
     gene            5388..5855
                     /gene="slyB"
                     /locus_tag="OO544_002167"
     CDS             5388..5855
                     /gene="slyB"
                     /locus_tag="OO544_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HCR6767757.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            complement(5902..6336)
                     /gene="slyA"
                     /locus_tag="OO544_002168"
     CDS             complement(5902..6336)
                     /gene="slyA"
                     /locus_tag="OO544_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HCR6767758.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            6537..6773
                     /locus_tag="OO544_002169"
     CDS             6537..6773
                     /locus_tag="OO544_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HCR6767759.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            6776..7633
                     /locus_tag="OO544_002170"
     CDS             6776..7633
                     /locus_tag="OO544_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR6767760.1"
                     /translation="MSIKTIKYFSTIIVAVVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     RVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            7654..9645
                     /locus_tag="OO544_002171"
     CDS             7654..9645
                     /locus_tag="OO544_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001376876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCR6767761.1"
                     /translation="MRNLPWFRATLAQWRYALRNTIAMCLALTVAYYLNLDEPYWAMT
                     SAAVVSFPTVGGVISKSLGRIAGSLLGAIAALLLAGHTLNEPWFFLLSMSAWLGFCTW
                     ACAHFTNNVAYAFQLAGYTAAIIAFPMVNITEASQLWDIAQARVCEVIVGILCGGMMM
                     MILPSSSDATALLTALKNMHARLLEHASLLWQPETNDAIRAAHEGVIGQILTMNLLRI
                     QAFWSHYRFRQQNARLNALLHQQLRMTSVISSLRRMLLNWPSPPGATREILEQLLTAL
                     ASSQTDAYTVARIIAPLRPTNVADYRHVAFWQRLRYFCRLYLQSSQELHRLQSGVDDH
                     ARLARTSALARHTDNAEAMWSGLRTFCTLMMIGAWSIASQWDAGANALTLAAISCVLY
                     SAVAAPFKSLSLLMRTLVLLSLFSFVVKFGLMVQISDLWQFLLFLFPLLATMQLLKLQ
                     MPKFAALWGQLIVFMGSFIAVTNPPVYDFADFLNENLAKIVGVALAWLAFAILRPGSD
                     ARKSRRHIRALRRDFVDQLSRHPTLSGSEFESLTYHHVSQLSNSQDALARRWLLRWGV
                     VLLNCSHVVWQLRDWESRSDPLSRVRDNCISLLRGVMSERGVQQKSLAATLEELQRIC
                     DSLARHHQPAARELAAIVWRLYCSLSQLEQAPPQGTLAS"
     gene            complement(9646..10167)
                     /gene="sodC"
                     /locus_tag="OO544_002172"
     CDS             complement(9646..10167)
                     /gene="sodC"
                     /locus_tag="OO544_002172"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC2"
                     /protein_id="HCR6767762.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGTGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(10248..11144)
                     /locus_tag="OO544_002173"
     CDS             complement(10248..11144)
                     /locus_tag="OO544_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HCR6767763.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RDHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLKSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            complement(11193..11432)
                     /locus_tag="OO544_002174"
     CDS             complement(11193..11432)
                     /locus_tag="OO544_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HCR6767764.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            11535..12134
                     /gene="nemR"
                     /locus_tag="OO544_002175"
     CDS             11535..12134
                     /gene="nemR"
                     /locus_tag="OO544_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HCR6767765.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKIAEVPKGS
                     FYHYFRSKEAFGVAMLERHYATYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGHENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRNSEPLENALAHVKTIIATPAV"
     gene            12171..13268
                     /gene="nemA"
                     /locus_tag="OO544_002176"
     CDS             12171..13268
                     /gene="nemA"
                     /locus_tag="OO544_002176"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HCR6767766.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQRRAELNPQRAESFYGGGAEGYTDYPTL"
     gene            13349..13756
                     /gene="gloA"
                     /locus_tag="OO544_002177"
     CDS             13349..13756
                     /gene="gloA"
                     /locus_tag="OO544_002177"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HCR6767767.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            13859..14506
                     /gene="rnt"
                     /locus_tag="OO544_002178"
     CDS             13859..14506
                     /gene="rnt"
                     /locus_tag="OO544_002178"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HCR6767768.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            14599..19168
                     /locus_tag="OO544_002179"
                     /pseudo
     CDS             14599..19168
                     /locus_tag="OO544_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
     gene            complement(19219..19566)
                     /gene="grxD"
                     /locus_tag="OO544_002180"
     CDS             complement(19219..19566)
                     /gene="grxD"
                     /locus_tag="OO544_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HCR6767769.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGKLVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
BASE COUNT         4535 a         5215 c         5112 g         4807 t
ORIGIN      
        1 aaaacggtga cgattacttt gccgttaacc ctaaggggca ggtgccagca ttgctgctgg
       61 atgacggtac tttgctgacg gaaggcgtag cgattatgca gtatcttgcc gacagcgtcc
      121 ccgaccgcca gttgctggca ccggtaaaca gtatttcccg ctataaaacc atcgaatggc
      181 tgaattacat cgccaccgag ctgcataaag gtttcacacc tctgtttcgc ccagatacac
      241 cggaagagta caaaccgaca gttcgcgcgc agctggataa gaagctgcaa tatgtgaacg
      301 aggcactgaa ggatgagcac tggatctgcg ggcaaagatt tacaattgct gatgcctatc
      361 tgtttacggt tctgcgctgg gcatacgcgg tgaaactgaa tctggaaggg ttagagcaca
      421 ttgcggcatt tatgcaacgt atggctgaac gtccggaagt acaagacgcg ctgtcagcgg
      481 aaggcttaaa gtaatagcca tcggcccaca ggtcgttgtg ggccgaaatg tgatatttca
      541 gagctttgtt gcgctgaagt aatgttctgg tttggcaata cgatcctgag cagccaccac
      601 ttgcagctca tattcctgca ttgctttggt ggtcaccatg atttcgtaga ctgcagcggt
      661 cacatgttcc aggacctcct gcagcgttgc cccctgaagc agtttcacca gcagtaaacc
      721 gctcgtcaca tcaccaacac ctaccggctg gcgcatacca aaatccacca gtggacggct
      781 gatatgccag gcttcatcgg cggtgaccag cagcatttca aaacggtcac ggctgtagcc
      841 agctcgcgcc aggtgtttaa ccaacacaat ttgtggccct tgcgcaatga gttcgcgcgc
      901 tgccagaacc gcttcttcga cgttatttac cgcatgctca cagagtattt ccagctcaac
      961 cagattcggc gcaatgatat cgctggcagg caaaccgtgc cgcacatgaa actctgcgac
     1021 acccggtgca acgatacagc ctttttccgg atgccccatt accggatcgc aaaaatattt
     1081 cgcctgcgga ttcgctgctt tcacctgacg gacgataccg aggatatgtt caccctgctc
     1141 cgccgatccc agatagccac ttaacacggc atcacaggtg tgtaatttat caatagcggc
     1201 aatgccttgc acaatttcgg ttaaatggct gggcggcatc acgcagccag tccatttgcc
     1261 gtattgggtg tgattagaaa attgaacggt gttcagcggc cagacgttcg cgcccaggcg
     1321 gcgcatcgga aactctgccg cactgttacc cgcatgacca taaacaacgt gagactggat
     1381 agcgagaata tttttcatca tgttccctgt ataaaaacca gggggagtga tttctcactc
     1441 cccctttcca cttaatgcat tatttccagc aaatcagaca gtaattcttt ttgccgcgac
     1501 gcagtaaggt aaaacgacca aacagacgat cttcttcttt aaagaagtat tcaggatcgg
     1561 actgtttttc accgttaatg gtgatggcat tggaggcgat agttttacgc gcctgaccac
     1621 gggaaggttg cagttcagaa tcgaccagcg cctgcatcag gtctgcgccc ttttccatct
     1681 caaccatcgg tacgccgtcc tgcgccagct gttcgaagtc cgcttcactc agcgcactca
     1741 aggaaccgct gaacaggcat tcggtaatac gttttgccgc ctgcaaacct tcttcaccgt
     1801 gaaccagacg agtcacctgc tccgccagta catactgggc gcgcggtgct ttaccgctgt
     1861 ttttatcttc ttcttccagg gcgttgatct cttcaatgct cataaaggtg aagaacttca
     1921 ggaagcggta aacgtcggca tccgcagtgt tgatccagaa ctggtagaat ttgtacgggc
     1981 tggttttctt cggatccaac cagactgcgc cgccttcagt tttaccaaat ttggtgccgt
     2041 ctgctttggt gatcagcgga acggtcaggc caaacacctg attctgatgc agacgacggg
     2101 tcaggtcgat accagaagtg atgttacccc actggtcaga accaccaatt tgcagcacca
     2161 caccgtactg tttgttcaga caggcgaagt cataaccctg caacaggttg taggaaaact
     2221 cagtgaacga aatcccctga tcttcacggt tgagacgctg cttaaccgct tctttgttga
     2281 tcatctggtt aacggagaag tgtttgccaa tatcgcgcag gaaggtcagc acattcatat
     2341 tgccgaacca gtcatagttg ttcgccgcga tagcagagtt ttctccacag tcgaaatcaa
     2401 ggaacggggc aacctgcttg cggattttgt ccacccactc ctgaacagtt tcttcggtgt
     2461 tcagcttacg ctcggcagct ttgaagctcg ggtcgccaat cagacccgtc gcgccgccta
     2521 ccagcgcaac cggcttgtgg cccgcctgct ggaagcgttt caggcataac aatggaacaa
     2581 gatgccccaa atgcaagctg tcagcggtag gatcgaagcc gcaatagagc gcgatcgggc
     2641 cttgcgccag tcgctctgct aacgcttcct cgtccgtcac ctgggctacc agcccccgct
     2701 cttgcaattg tttaatcaag ttactgcttg ccatcaaaat ctccatgtat ataacgactg
     2761 cacctttgcc ggtacacgac ttttcgccag atgcgaaaga gacatagaat aaagtgccag
     2821 aatcaggagt accagcgatt aaagcaagat ttttgcatct tttcagggtg caagacgatc
     2881 aatcttccac gcatcatttt cacgctggta caaaaagcgg tcatgcaggc gatgctcacc
     2941 gccctgccag aactcaatct gttcaaggct gacgcgaaaa ccgccccaaa agctcggcaa
     3001 tggcacttcg ccctgttgaa acttctgctt cagctccagg aatttacttt caaggatacc
     3061 gcgggcagaa atgcgactgg actgcttcga aacccatgca ccaatctggc tatcacgcgg
     3121 gcggctatga aaatatttca tcacttcgag agtcgaaagt cgttccgctt taccgatcac
     3181 catcacctgg cgctcaaggg tatgccacgg gaacagcagg ctaacgcgcg gattattttc
     3241 aatttgatgt gctttacggc tgccgaggtt ggtgtaaaac accatgcctt tttcgtcgta
     3301 atgtttgagt aaaacgatgc gttgataagg ctgagcatgt tcatccacgg tagcgaccac
     3361 catcgcggta gggtctgcca gtttggcttc acacgcctga gagagccaac gttcaaaaag
     3421 ggttaatgga tcggcgggaa gatcgcgacg gcgtaacccg cctttggtgt attcacggcg
     3481 cagatgcgcg atttgctgca attcgtcgtt atcagacatg gttttcttta cggattgtca
     3541 gtgggtgacg ctattgtgcg ccgcccctgg aaaaatctca acgctgtgga ttttgtaact
     3601 gacagttatt caatacgatg cggtcgcgtt tatagacagt cgcttcatcg cctttcgacc
     3661 agaaaacata gattccgtca gtgtaacgta caccagacgc tgaaatgccc tgtttgagat
     3721 gcagtagttg attatcgtaa acaaaactga cttcctggcg cggattattc agtttgaccg
     3781 tcaacggttt ttcatcacac tggtattcca gcgtagcggt ttgcatgtgc tcaaccagtt
     3841 gattgaacgc gctacagccg gtgagaagca ccggcaaaca gatcagtaac agttttttca
     3901 tagacatttc ccgaagactt tcctggtctg gagggcaata cgccctccct aacgtttcaa
     3961 gtgtaacggc agacgcggta agaaaaattc agttaactct gatatcacgg gttagcaggg
     4021 aaaatagccc ccagtaccgt ctcctggctt gcgccagtga cggaaggcag atttcctggt
     4081 aatcccgcca gcgtccgcca ggcaagccag gcgaatgcca atgcttccat gtcatcgcca
     4141 ctaatgccaa cggcatcggt ggtggtgact tctgtgcctg gcagtaatgc cgccagacgc
     4201 gccatgagta gcgggttacg actacctcca ccacatacca tcaatcgttc gcagccaccg
     4261 ctcaacaaaa cttgttcaga aatggtcacg gcggtgagtt ctgccagtgt ggcctgaaca
     4321 tcacggggat caacacccgg aaaatggcgt aaatggcgct ccagccagcc gtagttaaag
     4381 tattcgcgtc cggtgctttt cggtgcaggt tgcgagaaat atgggtcgct gagcatattt
     4441 tgcagcagtg ggagaataac tttacccgcc cgtgcccact cggcatcttt atcgtaaggt
     4501 ttaccggcct gacgccagat ccaggcatcc atcagcatgt taccaggacc ggtatcgtag
     4561 ccgccaaccg gctgcccagg aatgagcagt gacagattgg cgatgccgcc aatattgagc
     4621 accattcgtc gctcggttgg gtgagccagc aaggcatgat ggaacgcagg taccagcggt
     4681 gcgccttgtc ctcccaaggc aatatcgcga cggcgaaaat caccgacaac cgtaattccg
     4741 gtgcgtgcca caatttgatt gttatcgcca atctgtaaag tgtgtggtgc cacgcccgtc
     4801 ggttcatgcc agacggtttg accgtgacag ccgatcgcaa ctatatctct cgcctgcagg
     4861 ttttgttcct taagcaaggc attgaccgca tcagcaaaaa gtcgcccgag ttgagtatca
     4921 agctgtccaa actgcgaaag tgtaagctgc tggccctggc aaatatccag tacagcctgt
     4981 ttcagagaca ccgggatcgg ccagctcaag cttgccagct gtgcgacccg gtgttcatct
     5041 attgtcgcca acacaacatc aacaccatca aggctggtgc ctgacataac gccaataaag
     5101 cggcccgatt tcatagttca tcctttttca atctgacgtt tgcgcaccac tcaaacataa
     5161 acttttcgtg gataccatgc ggaatgaccg atttttaccg ttggtagtaa aacattatct
     5221 tcaaatcaat aatcatcatg aatgttttgt ttataattgg ttgatcctac tttcattatg
     5281 atttgctcat atttggtaga acatgtaacc atggattcac atatgccata tactttgacc
     5341 atgagggatg cttgcgtggc gtttcatggt gaacaggaga tttttcaatg attaaacgcg
     5401 tattggttgt ttcaatggta ggtctgtctc ttgtcggttg tgttaataac gacaccctgt
     5461 caggggatgt ttataccgct tctgaagcga aacaagtaca gaatgtcagc tatggcacca
     5521 tcgttaacgt acgtccggta cagattcagg gcggtgatga ttccaacgtt atcggtgcaa
     5581 ttggcggtgc tgttcttggt ggtttcctgg ggaatactgt tggtggcgga accgggcgtt
     5641 ctctggctac tgcagcaggc gctgttgcag gtggcgtagc tggtcagggc gtacagagtg
     5701 caatgaacaa aacgcagggt gtcgagctgg aaattcgtaa agacgatggt aataccatca
     5761 tggtggtaca gaaacaaggc aacactcgtt tctctccggg ccaacgtgtc gtactggcca
     5821 gcaatggcag tcaggtgacc gtttctccgc gctaaataag tttgcgtgtg gtcaggttac
     5881 tgaccacacg cccccttcat ttcacccttt ggcctgtaac tcaatgatat tatgctcaag
     5941 ttttgcgatg agcgtaatca gttgctccag ttcctctgcg gagatgccat gtaatatttc
     6001 cgcgcgggtt ttgttaataa cagcttccat ttcgctgatc agtggctctg ccttttccgt
     6061 cagtttaata cgtttagccc gacgatcgct ggcacaagtt tgacgcgaaa ttaacccttt
     6121 ttcttccagt tggtccagag tacggaccag tgatggctgc tcgatgccaa tcgcttttgc
     6181 cagttgaatt tgcgactggt ctggaggtaa ctgatggata ttgtgtaacg taacccaatg
     6241 ggtttgtgtt aactccagcg gtttcaggcg atggtctatc agagcacgcc atatgcgcac
     6301 caaccgtgcc agatcagaac ctagtggcga ttccaatttc atctccttat aattagcttg
     6361 ctaagatatt atgcggcttt tagaatagtg tgcagcaatt gtattgctaa aacaaatgta
     6421 ttgctgcatt tggttaccgt cagacatatt tttcagaaat tgcgcgtaaa tttttcgcac
     6481 ttaaagaata tttattaatc taacgcaata tattcggtcg taaaggaatc tactttgtga
     6541 agtttatgct caatgcaaca ggattgccct tacaagatct ggtgttcggt gcgtccgtct
     6601 attttcctcc gtttttcaaa gcattcgcgt ttggattcgt catctggctt gtcgtacacc
     6661 gcctgcttcg tggctggatc tacgccggtg acatctggca tcccttgtta atggatttat
     6721 cgctgtttgc gatttgcgtt tgccttgctc tggcaatact gattgcgtgg taactatgtc
     6781 aattaaaaca attaagtatt tctcaacaat cattgtagcg gtagttgcgg ttcttgccgg
     6841 atggtggctg tggaattatt acatgcaatc tccgtggaca cgcgacggga aaatacgcgc
     6901 agaacaggtt tctatcaccc cacaggtgtc gggacgtatt gttgagctga atataaaaga
     6961 caaccagctg gttaacgcag gggatcttct gctcacgatc gataaaacgc cctttcagat
     7021 cgccgagctg aacgcacagg ctcagctggc gaaagcgcaa tctgacctcg caaaagccaa
     7081 taacgaagcg aatcgtcgtc gtcatctctc acaaaatttt atttctgccg aagaactcga
     7141 taccgctaac ctcaatgtta aagcgatgca ggccagtgtt aatgccgcac aggccacgct
     7201 gaaacagaca caatggcaac tggcgcaaac ggaaattcgc gccccggtga gtggacgggt
     7261 gactaacctc actacccgca tcggcgatta cgctgacacc gggaaaccgc tttttgccct
     7321 tgtcgacagc cactcgtttt acgtcattgg ttattttgaa gaaaccaagt tgcgccatat
     7381 ccgcgaaggt gcaccagcac aaattaccct ctatagtgat aacaaaacgt tacagggtca
     7441 cgtttccagt atcggtcggg cgatttatga tcagagcgtt gaaagtgact caagccttat
     7501 tccggatgtg aaacctaacg tcccctgggt tcgtctcgcc caacgcgttc ccgtccgctt
     7561 tgcgctggat aaagttccag gcgatgtcac gctggtgtcg ggcaccacct gtagcattgc
     7621 cgtaggtcaa taatgaacgc atcgtaatgg tccttgcgca atttgccctg gttcagggcc
     7681 acgctggcgc aatggcgtta tgcgttacgc aataccattg ccatgtgtct ggcgctgacg
     7741 gttgcctatt atttgaatct ggatgaaccc tattgggcga tgacctcggc tgcagtggtt
     7801 agctttccca ccgttggcgg tgtcatcagc aaaagcctcg gacgcatcgc tggcagttta
     7861 ctgggagcca ttgcggcact gcttcttgcc ggacatacgc tcaatgagcc gtggtttttt
     7921 ctattgagca tgtcggcgtg gcttggcttt tgtacctggg cctgtgcgca cttcacgaat
     7981 aacgtcgcgt atgcatttca actggcgggc tacacggctg ccatcatcgc ctttccgatg
     8041 gttaatatta ctgaggccag ccagctgtgg gatatcgctc aggcgcgcgt ttgcgaggtg
     8101 atagtcggta ttttgtgcgg cggcatgatg atgatgatcc tgcctagcag ttccgatgct
     8161 actgcccttt taactgcatt gaaaaacatg cacgcccgat tactggaaca tgccagttta
     8221 ctctggcagc ctgaaacaaa cgatgccatt cgtgcagcac atgagggggt gattgggcag
     8281 atactgacca tgaatttgct gcgtatccag gctttctgga gccactatcg ttttcgccag
     8341 caaaacgcgc gccttaatgc gctactccac cagcaattac gtatgaccag tgtcatctcc
     8401 agcctgcgac gtatgttgct caactggccc tcaccgccag gtgccacgcg agaaattctc
     8461 gaacaattgc tgactgcgct cgccagttcg caaacagatg cttacaccgt cgcacgtatt
     8521 atcgccccgc tacgcccgac caacgtcgcc gactaccggc acgtcgcctt ctggcagcga
     8581 ctacgttatt tttgccgcct ttatctgcaa agtagtcagg aattacatcg tctgcaaagc
     8641 ggtgtagatg atcatgccag actcgcacgg acatccgctc tggctcgtca taccgacaac
     8701 gccgaagcta tgtggagcgg gctgcgtaca ttttgtacgt tgatgatgat tggcgcatgg
     8761 agtattgcat cgcaatggga tgccggtgcc aatgcattaa cgctggcagc aattagctgc
     8821 gtactctact ccgccgtcgc agcaccgttt aagtcgttgt cactgctgat gcgcacgctg
     8881 gtgttacttt cgctattcag ctttgtggtc aaatttggcc tgatggtcca gattagcgat
     8941 ctgtggcaat ttttactgtt tctctttcca ctgctggcga caatgcagct tcttaaattg
     9001 cagatgccca aatttgccgc actgtggggg caactgattg tttttatggg ttcgtttatc
     9061 gctgtcacta atcccccggt gtatgatttt gctgattttc ttaacgagaa tctggcaaaa
     9121 atcgttggcg ttgcgctggc ttggctagcg ttcgccattc tgcgtccagg atcggatgct
     9181 cgtaaaagcc gccgccatat tcgcgcgctg cgccgggatt ttgtcgatca gctaagccgc
     9241 catccaacac tgagtggaag cgaatttgaa tcgctcactt atcatcacgt cagtcagttg
     9301 agtaacagcc aggatgcgct ggctcgccgt tggttattac gctggggtgt agtgctgctg
     9361 aactgttctc atgttgtctg gcaattgcgc gactgggaat cgcgttccga tccgttatcg
     9421 cgagtacggg ataactgtat ttcactgttg cggggagtga tgagtgagcg tggcgttcag
     9481 caaaaatcac tggcggccac acttgaagaa ttacagcgga tttgcgacag ccttgcccgt
     9541 catcatcaac ctgccgcccg tgagctggcg gcaattgtct ggcggctgta ctgctcgctt
     9601 tcgcaacttg agcaagcacc accgcaaggt acgctggcct cgtaattact taattacacc
     9661 acaggcatag cgttcaccgc caccgcccag cggtttaggt tgatcggaca tattatcgcc
     9721 gccaacgtgg accatcagcg ccttgtcttt gatttcatcc agtgatttca gacgaggcgc
     9781 gatgacggca tcggtagctt tgccgtcatt attgacgacc agtgcaggca gatcgcctaa
     9841 atgcccggta ccttctggcc cttcatgttt accggtattt tgtggatcaa gatgcccgcc
     9901 tgcggattcc gcggcgctgg ctttgccatc tttggtggct ggctggcagc ttcctttggc
     9961 atgaatatgg aagccatgtt caccgggggg taatgctttc agatcgggtg aaaactccag
    10021 acctttatcg gtttcagtaa tggtgacgct accaattgac tgcccaaccc cttgcgacgt
    10081 gacgaggttc atctcgactt tttcactggc agcttgtgcg ccggtcgcaa caaccagcgc
    10141 cagaatggcc agactaaaac gtttcatagg acctccgttc attgtgcatt cctgaataag
    10201 tgtaaaccag tgacacattt ttgaacggcg gctattccta aaagtgctta cggtacgtcg
    10261 taccccagtg ccgctttacg gatacgaaac cattgttgac gggtcatttt cagtgtttct
    10321 gcttcgacag ctgcccgtac gcgctcaatt ttacctgagc cgataattgg cagcggctgc
    10381 gacggtaaac gtaataccca ggcgtaaacc acctgttcaa tcgagcccgc gtttaactcc
    10441 tccgccacca cagccagttc atcacgcagc ggctggaaat aatcatcatt aaacagacga
    10501 ccaccaccaa ggcaggacca cgccatcgga cgaacacgca gttgttgtag ttggtcgagc
    10561 gtgccatcca gcagtaacgg ctgatgcacc ggggatattt ccacctgatt agtggcaagg
    10621 gtaaacggca gacgtgattt caacagggca aattgcgcag gcgtaaagtt cgatacgcca
    10681 aaatgacgca ctttgccgct ctgatgcaga tgtttgaacg cgtccgccac ttcatcggca
    10741 tccattaacg ggtctggtcg gtggattaac agcagatcca gatgatcggt cgcgagatta
    10801 attagcgact gttcggcgct cttaatgatg tgatcgcggt cagtgatgta atgaccaatg
    10861 acgttttctt cacgcgcggt cgtcgcgata ccgcatttac tgacgatttc catccgttca
    10921 cgcaggtgag gtgccagttt cagtgcctcg ccaaacgccg cttcgcactg atagccacca
    10981 taaatatcag catggtccac ggtggtcacg ccgagatcca gatgctcttc aataaaactg
    11041 accagctggc gggcggacat attccagtcc atcaatcgcc agtagcccat cacaaaacgg
    11101 gaaaactccg ggccttgcgg cgcaatagta atacgctgaa ccataatcgc ttcctcttat
    11161 cagatatgag aggagtatac gcaagattag gttcaaaaga gtgatggttg ctccggttcg
    11221 tctgatgacg ctggcttatt tgcgcgtaat ttgcgcatta atcgctgccg acaaaggcgc
    11281 agcacctctt gtttttcgcc atcgctcatt ttattccagt taaaacgctc atcccgacta
    11341 cgaaaacagc cgcgacaaaa cccgcgttcg tcagactggc aaattccccg gcacgggctc
    11401 tggacgggaa agaactctaa ttgctccgcc acttcgccct cctcaaataa gattattacc
    11461 attattgaag ctgttaatgt ccaaagtagc aactttgctt gcactagacc gactggtcta
    11521 ctacactcca acgcatgaac aaacacaccg aacatgatac tcgcgaacat ctcctggcga
    11581 cgggcgagca actttgcctg caacgtgggt tcaccgggat ggggctaagc gaattactaa
    11641 aaatcgctga agtgccgaaa gggtccttct atcactactt tcgctctaaa gaagcgtttg
    11701 gcgttgctat gcttgagcgc cattacgcca catatcacca gcgactgact gagttgctgc
    11761 aatccggcga aggtaactac cgcgaccgca tactggctta ttaccagcaa acactgaacc
    11821 agttttgcca acatggaacc atcagtggtt gcctgacagt aaaactctct gccgaagtgt
    11881 gcgatctgtc agaagatatg cgtagcgcga tggataaagg cgctcgcggc gtgattgccc
    11941 tgctctctca ggcgctggaa aatggccatg agaaccactg tttaaccttt tgtggcgaac
    12001 cgctgcaaca ggcacaagtg ctttacgcac tatggctggg tgcgaatctg caggccaaaa
    12061 tttcgcgcaa ttccgagcca ctggaaaacg cgctggcaca tgtaaaaacc attattgcga
    12121 cgcctgccgt ttagcaggca ttttttatca ccagacgacc gggagccttt atgtcatctg
    12181 aaaaactgta ttccccactg aaagtgggcg caataactgc ggcaaaccgt atttttatgg
    12241 caccgctgac gcgtctgcgc agtattgaac cgggtgacat ccctaccccg ttgatggcgg
    12301 aatactatcg ccaacgtgcc agtgccggtt tgattattag cgaagccacg caaatttctg
    12361 cccaggcaaa agggtatgct ggtgcacctg gcatccatag tccggaacaa attgccgcat
    12421 ggaaaaaaat taccgctggc gttcatgctg aaaatggtca tatggccgta cagctgtggc
    12481 acactggacg catttctcac gccagcctgc aacctggcgg tcaggcaccg gtagcgcctt
    12541 cagcacttag cgcgggaaca cgtacttctc tgcgcgatga aaatggtcag gcgatccgtg
    12601 ttgaaacatc catgccgcgt gcgcttgaac tggaagagat tccaggtatc gtcaatgatt
    12661 tccgtcaggc cattgctaac gcgcgtgaag ccggttttga tctggtagag ctccactctg
    12721 ctcacggtta tttgctgcat cagttccttt ccccttcttc aaaccatcgt accgatcagt
    12781 acggcggcag cgtggaaaat cgcgcacgtt tggtactgga agtggtcgat gccgggattg
    12841 aagaatgggg tgccgatcgc attggcattc gcgtttcgcc aatcggtact ttccagaaca
    12901 cggataacgg cccgaatgaa gaagccgatg cactgtatct gattgaacaa ctgggtaaac
    12961 gcggcattgc ttatctgcat atgtcagaac cagattgggc gggaggtgaa ccgtatactg
    13021 atgcgttccg cgaaaaagta cgcgcccgtt tccacggtcc gattatcggc gcaggtgcat
    13081 acacggtaga aaaagccgaa acgctgattg gcaaagggtt aattgatgcg gtggcatttg
    13141 gtcgtgactg gattgcgaac ccggatctgg tcgcccgctt gcagcgcaga gctgagctta
    13201 acccacagcg tgccgaaagt ttctacggtg gcggcgcgga aggctatacc gattacccga
    13261 cgttgtaatc caacattgcg agcggcgtaa agccgccgct atactaaaac aacattttga
    13321 atctgttagc cattttgagg ataaaaagat gcgtcttctt cataccatgc tgcgcgttgg
    13381 cgatctgcaa cgctccatcg atttttatac caaagtgctg ggcatgaaac tgctgcgtac
    13441 cagcgaaaac ccggaataca aatactcact ggcgtttgtt ggctacggcc cggaaaccga
    13501 agaagcggtg attgaactga cctacaactg gggcgtggat aaatacgaac tcggcactgc
    13561 ttatggtcac atcgcgctta gcgtagataa cgccgctgaa gcgtgcgaaa aaatccgtca
    13621 aaacgggggt aacgtgaccc gtgaagcggg tccggtaaaa ggcggtacta cggttatcgc
    13681 gtttgtggaa gatccggacg gttacaaaat tgagttaatc gaagagaaag acgccggtcg
    13741 cggtctgggc aactaatctc ctgccgggcg tgaactcatc gcgcccgcat ctttactgca
    13801 tcgacaagta atatttgtca taatgcgcgc tgcaatttat ccgtattaag agaatcagat
    13861 gtccgataac gctcaactta ccggtctgtg cgaccgtttt cgtggttttt atcctgttgt
    13921 gatcgatgtt gaaacagccg gatttaacgc caaaaccgat gcgctgcttg agattgccgc
    13981 catcaccctg aaaatggatg aacaaggctg gctgatgccg gacaccacat tacatttcca
    14041 cgtcgaacca tttgtcggcg caaatttgca gccagaggcc ctcgccttca acggcattga
    14101 cccgaacgat cccgatcgcg gcgcggtcag cgaatacgag gcgctgcacg aaatttttaa
    14161 agttgtacgt aaaggtatta aagcgagcgg ctgtaaccgc gccattatgg tggcgcacaa
    14221 tgccaatttt gatcacagct ttatgatggc cgccgcagaa cgcgcctcac tgaaacgtaa
    14281 cccgttccac cctttcgcca cttttgacac tgctgcactg gccgggctgg cactcggaca
    14341 aaccgtattg tcaaaggctt gccagaccgc tggcatggac ttcgacagca cccaggcgca
    14401 ctccgcgctg tacgacaccg aacgcactgc tgtgctgttt tgtgaaatcg tcaaccgctg
    14461 gaaacgtctg ggaggctggc cgctacctgc cgccgaagag gtgtaatcga gtcgatgcct
    14521 gatgacatgc aatgattcag gcatctatag tgaggctatt ccacgcatca agcatgatat
    14581 tcacggggaa tagcgttaat ggcagataat ccagaccctt catcgctcct gccggacgtg
    14641 ttttcactgg cgacccgcga ctggtttctt cgcgccttta aacagccgac cgctgtccag
    14701 tcgcaaacct ggcatgtggc ggcgcgaggc gaacatgcgc tggtgattgc accgactggc
    14761 tccgggaaaa cgctggcagc atttctctac gccctcgatc ggctcttccg cgaaggcggc
    14821 gaagataccc gcgaggcgca taagcgtaaa acctcacgca tcctctatat ttcaccgata
    14881 aaagccctgg gcaccgacgt tcagcgcaac ttgcagatcc cgttgaaagg tattgccgat
    14941 gaacggcggc ggcgcggcga aacggaagtc aatcttcgcg tagggatccg tactggcgat
    15001 acgcctgcac aggaacgcag caaactcacc cgtaatccgc cggatattct gatcaccaca
    15061 cccgaatcac tctatctgat gctgacctcc cgcgcgcgcg aaacgctacg cggcgtcgaa
    15121 acggtaatta ttgatgaagt ccacgcggta gcgggcagta aacgtggtgc gcatctggcg
    15181 ttaagtctgg agcggctcga tgcgctgctc cacacctcag cacagcgaat tggcctttct
    15241 gccactgtgc gctcagccag cgatgtggca gcatttcttg gtggcaatcg cccggttacg
    15301 gtagtcaacc cgcccgcaat gcgccatccg cagatacgaa ttgtcgtacc ggtcgccaat
    15361 atggatgatg tctcatcggt cgccagcagc accggcgaag acagccatgc cggccgggaa
    15421 ggctccatct ggccatatat tgaaacgggt atccttgatg aagtgttgcg ccatcgctcg
    15481 accattgtct ttactaattc acgtgggctg gcggaaaaac tgacggcacg attaaatgag
    15541 ctttacgccg cacgcttaca gcgttccccg tctatcgccg ttgatgcggc ccatttcgag
    15601 tcgacctccg gcgcaacctc taaccgtgta caaagtagcg acgtttttat tgcccgctca
    15661 caccacggct ccgtctctaa agaacaacga gcaatcaccg agcaggcgct gaaatcgggt
    15721 gagttacgct gcgtggtcgc aacctccagt ctcgaactgg ggattgatat gggcgcagtg
    15781 gatctgatga ttcaggtggc aacgccgctt tctgttgcca gtgggttaca acgcattggt
    15841 cgcgccggac atcaggttgg cggtgtatct aaagggctgt ttttccctcg tacccggcgt
    15901 gacttagtcg attccgcagt cattgtagag tgtatgttcg caggcaggct ggaaaacctg
    15961 acaccgccgc ataatccact cgacgtcctt gcgcagcaaa ccgttgccgc cgcggcgatg
    16021 gatgcattac aggtagacga atggtactcc cgcgtacgcc gtgccgcacc gtggaaagat
    16081 ctgccaagac gtgtttttga cgctacgctg gatatgcttt ccgggcgcta tccctctggc
    16141 gatttttctg cttttcgccc caaactggtc tggaaccggg agaccgggat attgaccgcc
    16201 cgacctggcg ctcaattgtt ggcggttacc agcggtagca ccattccgga tcgtggcatg
    16261 tatagcgtgt tattacccga aggtgaagaa caggccggtt cgcggcgggt gggtgaactg
    16321 gatgaggaga tggtatatga gtcgcgggtg aacgacatta tcactctcgg cgctacctca
    16381 tggcggatcc agcaaatcac ccgcgatcag gtgattgtga ctcctgctcc gggtcgttct
    16441 gcccggctcc ccttctggcg tggtgaaggt aacggacgtc cggctgaatt aggcgagatg
    16501 atcggcgatt ttcttcattt gctggcggat ggcgcgttct tttccgggac tattcccccg
    16561 tggctggcag aagaaaatac gatcgccaat attcaggggt tgattgacga gcaacgcaac
    16621 gcgacgggca tcgttccggg gagtcgccat ctggtcctcg aacggtgccg tgatgaaatt
    16681 ggcgactggc gtattatttt gcactctccc tatggaagac gggtgcatga accctgggcg
    16741 ctggcgattg ccgggcgaat acatgcccta tggggcgctg acgcgtctgt ggtcgccagt
    16801 gatgacggca ttgttgcgcg cattcctgac actgatggta aattgcccga cgccgcgatt
    16861 tttttgtttg aaccagaaaa gttgctgcaa attgtccgcg aggcggtagg cagctcggca
    16921 cttttcgccg cccgttttcg cgaatgcgcc gcgcgggcat tattaatgcc ggggcgcact
    16981 ccgggccatc gcaccccgct ttggcaacaa cggctgcgcg ccagtcagtt gctggaaatc
    17041 gctcagggat atccggattt tccggtcatt ctcgaaaccc tacgcgaatg tctgcaagat
    17101 gtttatgatc ttcccgcact ggaacgtttg atgcgtcgcc tgaacggtgg cgaaattcaa
    17161 atatccgatg taacgaccac tacgccctcg cctttcgcca caagtttatt gttcggctat
    17221 gtcgcggaat ttatgtacca gagcgacgcc ccgctggcag agcgccgggc atccgtactg
    17281 tcgctggaca gcgagttact gcgcaatcta ctcggacagg tcgatccggg ggaattactc
    17341 gacccgcagg tcattcgcca ggtggaagaa gagttgcaac gactggctcc tggcagaaga
    17401 gcgaaaggtg aagaaggatt gttcgacctg ctgcgcgaac tggggccaat gaccgttgaa
    17461 gacctggcgc aacggcatac aggcagcagt gaagaggttg cgtcgtatct ggaaaatctt
    17521 cttgcagtaa aacgaatctt cccagcgatg attagcggac aggagcgtct tgcctgtatg
    17581 gatgatgccg ccaggctgcg tgatgccctc ggcgtacgac taccagagtc attgccagag
    17641 atttatttac atagagtcag ttacccgctt cgcgacctct ttctgcgcta tctccgggct
    17701 catgctctgg tcacggctga acaactggct catgagttta gtctcggtat tgccattgtc
    17761 gaagagcagc ttcagcaact gcgtgaacag ggtctggtga tgaatctgca acaagacatc
    17821 tgggtgagcg atgaagtatt tcgtcgtctg cgtttgcgct cgctgcaagc cgccagagaa
    17881 gcgacgcgtc ccgttgcagc cacgacctat gcgcgattgc tgctggaacg tcagggcgta
    17941 ttacccgcca ccgatggtag cccggcgctc tttgcctcaa catcgccagg cgtttatgag
    18001 ggcgtagatg gcgtgatgcg ggtgatcgaa cagcttgccg gagtcggttt acccgcctca
    18061 ctctgggaaa gccagatcct gcctgcccgc gtacgcgact attcgtcaga aatgctcgat
    18121 gaattactgg caaccggtgc ggttatctgg tcggggcaaa aaaactgggt gaagatgacg
    18181 gcctggtggc actgcatcta caggaatatg ctgcagaatc gttcactccc gccgaagcgg
    18241 atcaggcgaa tcgttcggcg ctgcaacaag cgatagtcgc tgttctggct gacggaggag
    18301 cctggtttgc acaacaaatc agccaacgga tacgcgacaa aatcggcgaa tcggttgatc
    18361 tctctgccct gcaagaggcg ttatgggcgc tggtctggca aggcgtcatc accagcgaca
    18421 tttgggcacc gttacgcgcc ctcacccgca gcagttccaa cgcacgcacc tcaactcgcc
    18481 gcagtcaccg ggctcgtcgt ggacgtcctg tctatgcgca acccgtctcg ccgctggtat
    18541 cttacaacac accaaatctg gctggatgct ggcgctggcg gaaaatatgc tcgaccgcta
    18601 cggcatcatc agtcgtcagg cggtgatagc cgaaaatatc cctggcgggt ttccatcgat
    18661 gcaaacgctt tgtcgaagta tggaagactc cgggcgaatt atgcaaggtc gttttgtaga
    18721 aggtctgggt ggcgcgcaat tcgctgaacg tctgactatt gaccgattgc gcgatctggc
    18781 gacacaagcc acgcaaacgc gccactatac accagtggcg ctctctgcca acgatccggc
    18841 taatgtgtgg ggaaatcttc tgccctggcc tgcacatccg gcaacgctgg ttccaacgcg
    18901 tcgggcgggt gcgctggtgg tcgtttctgg cggcaaattg ttactctatc tggcgcaagg
    18961 tggcaaaaaa atgctggtct ggcaggaaaa agaggaatta ctcgccccag aggttttcca
    19021 cgcgctgact accgcactgc gtcgcgaacc acggctgcgc tttacgctaa cagaagtgaa
    19081 tgatctaccg gtccggcaaa cgccgatgtt tacgctgctg cgtgaggcgg gattttcaag
    19141 ttcgccacaa gggctggatt ggggatagag aaaggactga cggatgcccg ttcgcatccg
    19201 tcagtattgc aggacggatt attccgcgtc cggctcttca gacttgtatt tagcggcagt
    19261 ttctttgatc agctgctgca gttcgccacg ctgatacatt tcgatcacga tatcacaacc
    19321 gccgaccagc ttgccgtcaa cccacagttg cgggaaggtc ggccagttag catatttcgg
    19381 cagttccgca cgaatgtccg gattctgcag aatatcaaca taggcaaaac gttcgccaca
    19441 tgcggcaagc gcctggactg cctgggcaga gaaaccgcag ctcggcagtt tcggtgaacc
    19501 tttcatgtac agcaggatcg ggttttcagc aatctggcgt tggatttttt cgatagtggt
    19561 gctcattgtc ttgcttcctc aaacttcttt acggcagtaa tctgacattg tagcgggtca
    19621 gtgcggcatc ggaaaataac attttcatca cgcttttgct attttatcc
//
LOCUS       DAJTZL010000072        19407 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.072, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000072 DAJTZL010000000
VERSION     DAJTZL010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19407
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.072"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..245
                     /locus_tag="OO544_002181"
     CDS             <1..245
                     /locus_tag="OO544_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HCR6767770.1"
                     /translation="AHADYPGDEYQFGKEIAESELLWPVDRVQKSLNGELQQINGADY
                     FIFGHMMFDNIQTFANQIYIDTGTPKSGRLSFYKIK"
     gene            complement(296..1063)
                     /locus_tag="OO544_002182"
     CDS             complement(296..1063)
                     /locus_tag="OO544_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR6767771.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRMNVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            1259..2167
                     /locus_tag="OO544_002183"
     CDS             1259..2167
                     /locus_tag="OO544_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCR6767772.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGLSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVANAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            2164..3426
                     /locus_tag="OO544_002184"
     CDS             2164..3426
                     /locus_tag="OO544_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HCR6767773.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQQGCKQIYFKYCSTFDSTAKGNIGPVTDAL
                     MDALDTPFTVFSPALSVNGRTVYQGYLFVMNQLLAESGMRHHPVNPMTDSYLPRLVEA
                     QSTGRCGVVSAHVFEQGVDAVRQELARLQQEGYRYAVLDALTEHHLEIQGEALRDAPL
                     VTGGSGLAIGLARQWAQENGNQAREAGRPLAGRGVVLSGSCSQMTNRQVAHYRQIAPA
                     REVDVARCLSTETLAAYAHELAEWVLGQESVLAPLVFATASTDALAAIQQQYGAQKAS
                     QAVETLFSQLAARLAAEGVTRFIVAGGETSGVVTQSLGIKGFHIGPTISPGVPWVNAL
                     DKPVSLALKSGNFGDEAFFSRAQREFLS"
     gene            3423..4061
                     /locus_tag="OO544_002185"
     CDS             3423..4061
                     /locus_tag="OO544_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HCR6767774.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVAADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTALSCLQGLDSSNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAGEIAELRS"
     gene            4066..4842
                     /locus_tag="OO544_002186"
     CDS             4066..4842
                     /locus_tag="OO544_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HCR6767775.1"
                     /translation="MPRFAANLSMMFTEVPFIERFAAARKAGFDAVEFLFPYDYSTLQ
                     IQKQLEQNHLTLALFNTAPGDINAGEWGLSALPGREHEAHADIDLALEYALALNCEQV
                     HVMAGVVPAGEDAERYRAVFIDNLRYAADRFALHGKRILVEALSPDVKPHYLFSSQYQ
                     ALAIVEEVARDNVFIQLDTFHAQKVDGNLTHLIRDYAGKYAHVQIAGLPDRHEPDDGE
                     INYPWLFRLFDEVGYQGWIGYEYKPRGLTEEGLGWFDAWR"
     gene            4931..6295
                     /locus_tag="OO544_002187"
     CDS             4931..6295
                     /locus_tag="OO544_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family transporter"
                     /protein_id="HCR6767776.1"
                     /translation="MSTITLLCIALAGVIMLLLLVIKAKVQPFVALLLVSLLVALAAG
                     IPAGEVGKVMIAGMGGVLGSVTIIIGLGAMLGRMIEHSGGAESLANYFSRKLGDKRTI
                     AALTLAAFFLGIPVFFDVGFIILAPIIYGFAKVAKISPLKFGLPVAGIMLTVHVAVPP
                     HPGPVAAAGLLHADTGWLTIIGIAISIPVGIVGYFAAKIINKHQYAMSVEVLEQMQLA
                     PASEEGATKLSDKINPPGVALVTSLIVIPIAIIMAGTVSATLMPPSHPLLGTLQLIGS
                     PMVALMIALVLAFWLLALRRGWSLQHTSDIMGSALPTAAVVILVTGAGGVFGKVLVES
                     GVGKALANMLQMIDLPLLPAAFIISLALRASQGSATVAILTTGGLLSEAVMGLNPIQC
                     VLVTLAACFGGLGASHINDSGFWIVTKYLGLSVADGLKTWTVLTTILGFTGFLITWCV
                     WAVI"
     gene            complement(6389..7381)
                     /gene="rpoS"
                     /locus_tag="OO544_002188"
     CDS             complement(6389..7381)
                     /gene="rpoS"
                     /locus_tag="OO544_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HCR6767777.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(7444..8583)
                     /gene="nlpD"
                     /locus_tag="OO544_002189"
     CDS             complement(7444..8583)
                     /gene="nlpD"
                     /locus_tag="OO544_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HCR6767778.1"
                     /translation="MSAGSPKFTVRRIAALSLVSLWLAGCSDTSNPPAPVSSVNGNAP
                     ANTNSGMLITPPPKMGTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENG
                     RIVYNRQYGNIPKGSYSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVG
                     QTLQVGNASGTPITGGNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSA
                     NKMLPNNKPTATTVTAPVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASE
                     GGNKGIDIAGSKGQAIIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLV
                     REQQEVKAGQKIATMGSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(8723..9349)
                     /gene="pcm"
                     /locus_tag="OO544_002190"
     CDS             complement(8723..9349)
                     /gene="pcm"
                     /locus_tag="OO544_002190"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HCR6767779.1"
                     /translation="MVSRRVQALLEQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(9343..10104)
                     /gene="surE"
                     /locus_tag="OO544_002191"
     CDS             complement(9343..10104)
                     /gene="surE"
                     /locus_tag="OO544_002191"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HCR6767780.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(10085..11134)
                     /gene="truD"
                     /locus_tag="OO544_002192"
     CDS             complement(10085..11134)
                     /gene="truD"
                     /locus_tag="OO544_002192"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HCR6767781.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGAQRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEK
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(11131..11610)
                     /gene="ispF"
                     /locus_tag="OO544_002193"
     CDS             complement(11131..11610)
                     /gene="ispF"
                     /locus_tag="OO544_002193"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HCR6767782.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(11610..12320)
                     /gene="ispD"
                     /locus_tag="OO544_002194"
     CDS             complement(11610..12320)
                     /gene="ispD"
                     /locus_tag="OO544_002194"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HCR6767783.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(12339..12650)
                     /gene="ftsB"
                     /locus_tag="OO544_002195"
     CDS             complement(12339..12650)
                     /gene="ftsB"
                     /locus_tag="OO544_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HCR6767784.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(12844..13167)
                     /locus_tag="OO544_002196"
     CDS             complement(12844..13167)
                     /locus_tag="OO544_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HCR6767785.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            complement(13217..13822)
                     /gene="cysC"
                     /locus_tag="OO544_002197"
     CDS             complement(13217..13822)
                     /gene="cysC"
                     /locus_tag="OO544_002197"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HCR6767786.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(13822..15249)
                     /gene="cysN"
                     /locus_tag="OO544_002198"
     CDS             complement(13822..15249)
                     /gene="cysN"
                     /locus_tag="OO544_002198"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HCR6767787.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAPSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(15251..16159)
                     /gene="cysD"
                     /locus_tag="OO544_002199"
     CDS             complement(15251..16159)
                     /gene="cysD"
                     /locus_tag="OO544_002199"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HCR6767788.1"
                     /translation="MDQIRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            16411..17448
                     /gene="iap"
                     /locus_tag="OO544_002200"
     CDS             16411..17448
                     /gene="iap"
                     /locus_tag="OO544_002200"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HCR6767789.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKSWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTAAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            complement(17716..18009)
                     /gene="cas2"
                     /locus_tag="OO544_002201"
     CDS             complement(17716..18009)
                     /gene="cas2"
                     /locus_tag="OO544_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2"
                     /protein_id="HCR6767790.1"
                     /translation="MSMVVVVTENVPPRLRGRLAIWLLEVRAGVYVGDTSKRIREMIW
                     QQITQLAGCGNVVMAWATNIESGFEFQTWGENRRIPVDLDGLRLVSFLPVDNQ"
     gene            complement(18006..18929)
                     /gene="cas1e"
                     /locus_tag="OO544_002202"
                     /pseudo
     CDS             complement(18006..18929)
                     /gene="cas1e"
                     /locus_tag="OO544_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311636.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1"
     gene            complement(18926..>19407)
                     /gene="cas6e"
                     /locus_tag="OO544_002203"
     CDS             complement(18926..>19407)
                     /gene="cas6e"
                     /locus_tag="OO544_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HCR6767791.1"
                     /translation="FFVLSQERPAESDIFTIECRPFAPELRTGQSLCFNLRANPTICK
                     AGKRHDLLMEAKRQVRGQVEGSDVWLHQQQAALDWLAAQGERSGFTLLDTSVDAYRQQ
                     QLRRENSRQLIQFSSVDYTGMLTVTAPGLFLQRLSQGYGKSRAFGCGLMLIKPGAEA"
BASE COUNT         4592 a         5232 c         4847 g         4736 t
ORIGIN      
        1 tcgcacatgc agattatccg ggtgatgaat atcagtttgg taaagaaata gcagaaagcg
       61 aattactctg gcctgttgat cgtgtgcaga aatcgcttaa tggcgagtta caacaaataa
      121 acggcgctga ttattttata tttggacata tgatgtttga taacattcag acgttcgcta
      181 accagattta tattgatacc ggcacgccga aaagcgggcg gctgtcattt tataaaataa
      241 agtagtctca tgcttcttct gtgaagcatg agtaaccaaa tgttattgca ggccattatt
      301 catttttcgc taccagcaaa gagagatcct gcttcgccag cgcgcgactg gcactctccg
      361 gcaaaccgtc gtctgtgata atctgatcaa actcgcttaa tggtaacgcc agccatgtcg
      421 ccacctgacc atatttcgtc gcatcacaga ccaaaactcg ctggcggctg gcactggcaa
      481 tcgcccgttt caccgtgact ttatcttccg ctggcgtaga aatcccccgc acactccatg
      541 acgatgcaga aataaaagcc tgatcaatca tcaggctgcg cagcatggtc gcagcggctt
      601 ccccgacaca ggaacggttt tcccgacaca ctgcaccgcc agtgtgaata attgtgcaat
      661 tactgttgtc gagcaagtag ttcgcaataa cgaaatcgtt tgtgaccaca gtcagtgact
      721 ccatgtgaat cagatgctgt gctatcgcta acgtggtcgt tcccgcatcc agatagatac
      781 aacttcccgg ctgaacaaga cttgccgcca gcttgccaat agccgctttt tgcgtcattg
      841 ccagcgcagt ttttacctga tgagaaggtt catgcgccac gcgtcccgga gactggacgc
      901 ctccggacac cagcacaacg gctccctgct gctccagttt ttgtaaatcc cgacgaatgg
      961 tcatatgtga cacattcatt ctgtccgtta gttcagcaat actgacaatg cctttttcag
     1021 ctaccatctc aaggatgatt tggcgacgct ctacgggtat caacttttgc tccttccttt
     1081 gtcctgctga cattctacgc tatttgcctg cgaaacgtgc gcggcgcaac taacgcttag
     1141 ttcacataaa ataacacaca atgttaattt atgtgaatca gctcaccata tcgttatctt
     1201 ccagcgctta tattcacaat atcaaacaaa atatcactta aattaacaat gagagcagat
     1261 gaaaacggga tctgagtttc atgtcggtat cgttggctta gggtcaatgg gaatgggagc
     1321 agcactgtca tgtgtccgcg caggtctttc tacctggggc gcagacctga acagcaatgc
     1381 ctgcgctacg ttgaaagagg caggtgcttg cggggtttct gataacgccg cgacgtttgc
     1441 cgaaaaactg gacgctctgc tggtgctggt ggccaatgcg gcccaggtta aacaggtgct
     1501 gtttggtgaa acaggcgttg cacaacatct gaaacccggt acggcagtaa tggtttcttc
     1561 cactatcgct agtgctgatg cgcaagaaat tgctaccgct ctggctggat tcgatctgga
     1621 aatgctggat gcgccagttt ctggtggtgc agtaaaagcc gctaacggtg aaatgactgt
     1681 catggcctcc ggtagcgata ttgcctttga acgactggca cccgtgctgg aagccgttgc
     1741 cggaaaagtt tatcgcatag gtgcagaacc gggactaggt tcgaccgtaa aaattattca
     1801 ccagttgtta gcgggcgtac atattgctgc cggagccgaa gcgatggcac ttgcagcccg
     1861 tgcggggatc ccgctggatg tgatgtatga cgtcgtgacc aatgccgccg gaaattcctg
     1921 gatgttcgaa aaccggatgc gtcatgtggt ggatggcgat tacaccccgc attcagccgt
     1981 cgatattttt gttaaggatc ttggtctggt tgccgataca gccaaagccc tgcacttccc
     2041 gctgccattg gcctcaacag cattgaatat gttcaccagc gccagtaacg cgggttacgg
     2101 gaaagaagac gatagcgcag ttatcaagat tttctctggc atcactctac cgggagcgaa
     2161 atcatgatca agattggcgt tatcgccgat gattttaccg gcgcgacgga tatcgccagt
     2221 tttctggtgg aaaacggtct accaacggta caaattaacg gtgttccaac aggtaaaatg
     2281 ccggaagcaa tcgacgcact ggtgatcagc ctgaaaacgc gctcctgtcc ggtggttgaa
     2341 gccacacagc aatcgctggc ggctctgagc tggttgcaac agcaaggttg caaacagatc
     2401 tatttcaaat actgctctac tttcgacagt acggcgaaag gtaatattgg cccggttacc
     2461 gatgccttaa tggatgctct cgacacgccg tttacggtct tctctccggc cctgtcggtc
     2521 aacggacgta cggtttatca ggggtatttg ttcgtaatga atcaactgct ggccgaatcc
     2581 gggatgcgcc atcacccggt aaatcccatg accgacagct atcttccccg tctggttgaa
     2641 gcgcaatcca cagggcgctg cggcgtcgtt tcggcacatg ttttcgaaca aggcgtggat
     2701 gccgttcgtc aagagctggc tcgcttacag caagagggct accgctacgc ggtgcttgat
     2761 gcgctgaccg aacaccatct ggaaattcag ggagaagcct tgcgcgatgc cccactggta
     2821 acgggcggtt ctggtctggc gattggcctg gcccggcagt gggcgcaaga aaacggtaac
     2881 caggctcgcg aagcagggcg tccgctcgct gggcgcggcg tagtgctctc cggttcatgc
     2941 tctcaaatga ccaaccgcca ggtggcacat taccgtcaaa ttgcaccagc ccgtgaagtt
     3001 gatgtggcac gctgcctctc aaccgaaact ctggccgctt atgcacacga actggcagag
     3061 tgggttctgg gccaggaaag tgtacttgct ccactggttt ttgccaccgc cagcactgac
     3121 gcattggcag caattcaaca gcaatacggt gcacaaaaag ccagtcaggc agtagaaact
     3181 ctgttttctc aactagcggc gcggttagca gcggaaggcg tgacacgctt tattgtcgca
     3241 ggcggtgaga cctccggcgt agtcacacag agcctgggca taaaagggtt tcatattggc
     3301 ccaaccattt cccccggcgt gccgtgggta aacgcactgg ataagcctgt ctcactcgcc
     3361 cttaaatctg gcaacttcgg tgatgaagcc tttttttcac gagcccaaag agagttttta
     3421 tcatgagcga tttcgcaaaa gtagagcagt ctttgcgaga ggagatgacg cggattgcca
     3481 gttcattctt tcagcgcggc tatgcaaccg gttcggctgg caatctgtcg ctgcttttac
     3541 ctgacgggaa tttactggcg acaccgacag gttcatgcct gggcaatctc gatccgcagc
     3601 ggctttccaa agtcgccgcg gatggcgaat ggttaagtgg tgacaaaccc tcgaaagagg
     3661 tgctctttca tctggcgctg tatcgcaaca atccgcgctg taaagcggtg gtgcatttgc
     3721 acagcacatg gtcgacggcg ctttcctgcc tgcaagggct ggacagcagc aacgttattc
     3781 gtccgttcac accatacgtg gtgatgcgga tgggaaatgt cccgctggtg ccttattacc
     3841 gaccgggcga taaacgcatc gcacaggatc tggcggaact ggcagcagac aatcaggctt
     3901 ttttactggc aaatcatggc ccagtggttt gcggtgaaag cctgcaagaa gccgccaaca
     3961 atatggaaga gctggaggaa acggcaaagc tgatttttat tctcggtgac cgcccgatcc
     4021 gttatctgac cgcaggtgaa attgcggaat taaggagtta atgcaatgcc tcgttttgca
     4081 gctaatttat ccatgatgtt caccgaagtg ccttttattg aacgcttcgc cgcagcgcga
     4141 aaagccgggt tcgatgctgt ggaatttctg tttccctatg actactccac cctgcaaatc
     4201 caaaagcaac tggagcaaaa ccatctgaca ctggcgctgt tcaataccgc tcccggagat
     4261 attaatgcgg gggaatgggg attatccgcc cttcccggac gtgagcacga agcacacgca
     4321 gacatcgacc tggcactgga atatgcattg gcgctcaatt gtgaacaagt ccatgtgatg
     4381 gcaggcgtcg tgcccgctgg cgaagacgct gaacggtacc gggcagtatt tatcgataat
     4441 ctccgctacg ctgccgaccg ctttgcgcta cacggcaagc gaatattagt tgaagcactc
     4501 agccccgacg ttaagcctca ttatctcttc tccagccagt atcaggcact ggctattgtt
     4561 gaagaggttg cgcgagataa cgtgtttatt caactagata cttttcatgc gcaaaaagta
     4621 gatggcaacc tgacgcattt aatccgcgac tacgctggga aatatgcgca cgtacaaatt
     4681 gccggactac ctgatcgaca tgaaccggac gatggagaaa tcaactatcc gtggctgttc
     4741 cgcctgttcg atgaggtggg atatcagggc tggatcggtt atgaatataa acctcgtggc
     4801 ctcaccgaag aagggcttgg ctggtttgac gcctggcgct aattcgttaa atccctaatt
     4861 acaacgtacc catacatccc cccatctacg gatggggtag gttaactata actttcagac
     4921 agggtttccc atgtccacaa ttacattgtt atgcattgcg ttagctggcg taatcatgct
     4981 gttgctgctg gtcatcaagg caaaggtaca accattcgtt gctctgctcc tcgtcagcct
     5041 gttagtcgca cttgcggcgg gtataccggc gggcgaagtg ggtaaagtga tgatcgccgg
     5101 gatgggcggt gtgcttggtt ccgtcactat tattattggt ctgggcgcta tgctggggcg
     5161 tatgatcgaa cactctggtg gtgcagagtc actggctaat tatttcagtc gcaagttagg
     5221 tgacaaacga actatcgctg cgctgactct ggcagcgttc ttcctcggta ttcccgtctt
     5281 ctttgatgtc ggctttatta ttcttgctcc aatcatttac ggttttgcca aggttgccaa
     5341 aatatcgcca ctcaaatttg gcctgcctgt cgctgggatc atgctaactg tacatgtggc
     5401 ggtaccgccg catccaggcc ctgtcgccgc agcggggtta ctccacgcag acaccggctg
     5461 gctaaccatc atcggtattg cgatttctat tcccgtaggg attgttggct actttgcagc
     5521 gaaaataatc aataagcacc aatatgcgat gtcggtggaa gtactggaac agatgcaact
     5581 ggctccggcc agtgaggaag gcgcgacaaa attaagcgat aaaataaatc caccgggcgt
     5641 cgcgctggtc acctcgctaa ttgttattcc tatcgcgatt atcatggcgg gtacggtttc
     5701 cgcaacactg atgccgcctt cgcatcccct gcttggtacg ctacagctga tcggctcacc
     5761 aatggtagct ctaatgattg cgctggtgct ggcattctgg ttattggctt tacgtcgcgg
     5821 ctggagctta caacacacca gcgacattat gggctcagcc cttcccactg cggcggtagt
     5881 gattttggtt actggtgctg gaggggtatt tggcaaagtg ctggtggaat cgggcgtcgg
     5941 caaagccctt gccaacatgc tacaaatgat tgacctgcct ctgttaccag ccgcatttat
     6001 tatttcactg gcgctgcgtg catcgcaggg gtcagcaacc gtagcaatcc tgacaaccgg
     6061 cgggttactc tcagaagcgg tgatgggatt gaatccgatt cagtgcgtat tggtgacgct
     6121 ggcagcctgc tttggtgggc ttggggcgtc acatattaat gactcagggt tctggattgt
     6181 gaccaaatat ctggggttgt cggtagcaga cggtctgaaa acctggacag tgttaacgac
     6241 cattctcggt tttaccggat tcttaattac ctggtgcgta tgggcggtaa tttgacctta
     6301 ataaaaaggt ccgatgagca tcggaccttt tattgtgcac agaaaaggcc agcctcgctt
     6361 gagactggcc tttctgacag atgcttactt actcgcggaa cagcgcttcg atattcagcc
     6421 cctgcgtttg caggatttca cgcaaacggc gcaggccttc aacctgaatc tggcgaacac
     6481 gttcacgggt gaggccaatt tcacgaccta catcttccag tgttgccgct tcgtacccca
     6541 gcaaaccgaa tcgacgtgcc agtacttcac gctgtttggc gttcagctcg aacagccatt
     6601 tgacgatgct ctgcttcata tcgtcatctt gcgtggtatc ttccggaccg ttctcttttt
     6661 catcggccag gatgtccagc aacgcttttt cggaatcacc acccagcggg gtgtctaccg
     6721 aggtaatgcg ctcgttaaga cgaagcatac ggctgacgtc atcaactggc ttatccagtt
     6781 gctctgcgat ctcttccgca cttggttcat ggtccagctt atgggacaac tcacgtgcgg
     6841 ttcgcaggta aacgttcagc tcctttacga tgtgaatcgg caaacgaata gtacgggttt
     6901 ggttcataat cgcccgttca atcgtctggc gaatccacca agttgcgtat gttgagaagc
     6961 ggaaaccacg ttccgggtca aacttctcta ccgcgcggat cagccccagg ttgccctctt
     7021 cgataaggtc cagcaacgcc agaccacgat tgccataacg gcgggcaatt tttaccacca
     7081 gacgcaagtt actctcgatc atccggcggc gagaggcgac atctccacgc agtgcgcgac
     7141 gcgcaaaata aacttcttct tcggccgtta acagtggtga ataaccaatc tcaccaaggt
     7201 aaagctgagt cgcgtccaac acacgctgtg tggctccctg cgataacagt tcctcttcgg
     7261 ccaaatcgtt atcactgggt tcctcttcta ctaaggcctt ttcgtcaaaa acctcaactc
     7321 cgttctcatc aaattccgca tcttcattta aatcatgaac tttcagcgta ttctgactca
     7381 taaggtggct cctacccgtg atcccttgac ggaacattca agcaaaagcc tggttccgcc
     7441 gatttatcgc tgcggcaaat aacgcagcgg gtttacggat ttccccttgt aacgaatttc
     7501 aaaatgcaag cgtgttgaac tggttccggt gctacccatg gttgctattt tttgccccgc
     7561 cttcacttct tgttgttccc ggaccagcat tgtgtcgtta tgggcgtagg cactcaggta
     7621 atcatcatta tgtttgatga taatcagatt accgtagccg cgcagcgcgt taccggcata
     7681 aacaacgcgg ccatctgcgg tcgcgataat tgcctgtcct ttgctgcctg cgatatcaat
     7741 ccccttgttg cccccctcag aagcgccaaa ggtttcgatc actttgccct cagtcggcca
     7801 gcgccaggtg gagataggcg tactggttga tgtactgctg acagtcggct cggttgtgct
     7861 tgctgttggt accgttacag gcgctgtgac tgtggtcgca gttggcttgt tgttcggcaa
     7921 cattttgtta gcactctgtt cacccgaaga ctcagaatac gtaattgtcg gttgcgacgc
     7981 aacagcaacg gtggaatttt gtgcaggctt gatcacaact ccttgctctg ctgcgtcggc
     8041 ctgggtaatg gcatttccgc cagtgattgg cgtaccggaa gcattaccca cctgcaaggt
     8101 ctgaccaacg ttcagcgcgt atggtgcctg aatattgttg cgctgagcaa ggtcacggaa
     8161 atcgttgcca gtaatccagg cgatatagaa aagtgtgtcg ccttttttca cggtataggt
     8221 actgccgcta taactgcctt tcggaatgtt cccatactga cggttataga cgatgcgtcc
     8281 gttttccatc tgtaccggct gctgagctac tggctgcact ggctggattt gcggttgttg
     8341 agtagcctga atttgtggct gctgtaccgg ctgaatttgc ggttgctggg ctgtagacgt
     8401 cgtccccatt ttcggcggcg gcgtaatcaa cataccagaa ttagtatttg caggcgcatt
     8461 gccattaacg gagctgaccg gtgccggtgg atttgaagtg tcagaacagc ctgccagcca
     8521 tagcgaaacc agtgacaaag cagcaatgcg gcgaacggtg aattttgggc ttcccgcgct
     8581 catttatccc ccaggaaaaa ttggttaata accagtgaca taattaccgt gcaaggcacc
     8641 ctactgaaca ctggaaaaga tgttcacgat acgctgacct gcggcaaaat aaccaggaaa
     8701 aatccaggta tttcctcacg ttttaagcca gctcaccctt cactaaaggg acaaagcgca
     8761 cggcctccac ggtatcgata ataaattcgc ctccccgacg acgcacccgt ttcaaatact
     8821 ggtgctcctc ccctacgggt aagacgagaa tcccgccttc gtccagctgc gtcattagcg
     8881 cagttggaat ttccggcggt gccgccgtaa caatgatagc gtcaaacggc gcacgtgcct
     8941 gccaaccttg ccatccatcg ccatgacggg ttgaaacatt atgtaaatca agatttttca
     9001 ggcggcgacg tgcctgccac tgcaagcctt taatccgttc aaccgagcaa acatgctgga
     9061 caagatgcgc taggattgcc gtttgatatc ccgaaccagt gccaatttcc agcacccgcg
     9121 actgcggcgt cagctcgagt aattcggtca ttcgcgccac catatacggc tgcgaaattg
     9181 tctgcccctg acctatcggc aaggcgatat tgtcccaggc tttttgttca aacgcttcat
     9241 caacgaattt ttcacgcggc acggcggcaa gtgcattcag cacctgctca tcctgaatac
     9301 cttgcgcacg taattgctcc agaagtgctt gtacgcgtct gcttaccatt gcgtgccaac
     9361 tcccacgctg tttaaccagt ctgaaaccac atcttgcgcg ctatgcgcag ttaaatccac
     9421 atgcagcggc gtgatggaga catagccctc atctaccgca gcaaaatcgg tccccggacc
     9481 ggcatcacat ttaccgcccg gcgggccaat ccagtacagc gtattgccgc gcggatcttg
     9541 ctgcgggatc acctgatctg ccggatgtcg tgtaccgcag cgcgtcacgc gaataccttt
     9601 gatttgatcc aagggtaaat ccggaacgtt aatattaaga atacgtccgg tgcgcagcgg
     9661 ctctttacac agtgcgcgca aaattgaaca ggttaccgcc gcggcagtgt cgtaatgttt
     9721 atgcccgtca agcgagacgg ccagcgccgg aaaacctaaa tgacggcctt ccatcgcggc
     9781 ggctaccgta ccggaataaa taacgtcatc ccccagattc ggcccggcgt taatgccgga
     9841 cacaacaatg tccgggcgcg gacgcatcag agcattcacg ccaagataga cgcaatcggt
     9901 cggggttccc atttgcacag caatatcacc attttcaaag gtaaacgtgc gcagggagga
     9961 ttccagtgtc agagaatttg aagcgccgct gcggttacga tcgggggcga ccacctgaac
    10021 gtcagcaaac tcacgcaagg ctttcgccag cgtttgtata ccgggtgcat gtaccccgtc
    10081 atcattactc agcaatatgc gcataatcac ctgttgtgtt gataagttcc ctgacaacgc
    10141 tggttgcaaa actacccgcc ggaagccaga aacggatctc tacggtgacg tcatcccacc
    10201 aattccagct taattgttgc ggatacagca acatcgctct gcgcgcggct tcaacttttt
    10261 cgcgcaccag taaagcttgt aattcagttt ctgcggcgac agctgccttt tcgaatgcca
    10321 gcgcttcacg ctgagttccc cattcgccac tacccggcaa tgcggcggtt atcatcagct
    10381 ctttatcgtt gacgcgacgc tgtaattccg ccagttcttc ggtggttgcg acaaaccagc
    10441 taccacgtcc ggctaattgt agcgcatcgc cgtcaacaac ttgattaacg tctgcttttt
    10501 tgaggcgctc agcaacaatc tgattaaaca acgcactgcg ggctgccgac aaccaaaaac
    10561 tccgtttatt gcgatcgcgc accggagtat tggtttgcgc ccagcgctgc gccccctgca
    10621 agttgctacc gccaatccca aaacgttggg caccgaagta gttcggtaca ccttttacgc
    10681 aaatatcgat cagacgttgt tcaacgtcat cgcgattgct cacttcgcgc agaaccaggg
    10741 taaaggcgtt acctttcagc gcgcctaaac gcagcttgcg cttgtgccgc gcatactcca
    10801 gcacctggca gccttccagt tgaaaggcgc tcagatcggg catttccttg cctggcacgc
    10861 gagcgcataa ccactgttcc gtaacagcat gtttgtcttt ttgcccagcg aagctgactt
    10921 cacgggcatg aattttcagg aatttcgcca gtgcatccgc cacaaaacgg gtattgcagc
    10981 cgtttttgag gattctaacc agaatatgct caccttcacc atcaggctca aagcccaaat
    11041 cttccaccac cacaaagtct tccggattgg ctttcagcag cccggtgcct tgcggtttac
    11101 cgtggaggta agtgagatta tcaaactcaa tcattttgtt gccttaatga gtagcgccac
    11161 cgcttcacag gcaatccctt ccccacgtcc ggtaaatcca agtttttccg tagtagtggc
    11221 tttcacgtta acatcatcca tatggcagcc gagatcttcg gcaataaaca cgcgcatttg
    11281 tggaatgtgc ggcaacatct tcggtgcctg agcgataata gtgacatcga cgttgcccag
    11341 ggtataaccc ttcgcctgaa tacgacgcca ggcttcgcgt agcagctcgc ggctgtcggc
    11401 acctttaaat gccggatcgg tatccgggaa cagcttgccg atatccccca gcgccgccgc
    11461 gccaagcaat gcatcggtca acgcatggag cgccacgtcg ccatcagaat gcgccagcaa
    11521 tcctttttcg taaggaatgc gtacgccacc aatgataatt gggccttcac cgccaaaggc
    11581 atgtacgtca aaaccgtgtc caattcgcat tatgtattct cctgatggat ggttcgggtg
    11641 aggtaaaact cggccagtgc caaatcttcc gggcgcgtga ctttaatgtt atccgcacgg
    11701 ccttcgacca actgaggatg gaatccgcaa tattccagcg ccgaggcttc gtcggtaata
    11761 gtcgcgcctt catttagagc gcgcgtcaga cagtcatgta acagctcacg agggaaaaat
    11821 tgcggcgtca gcgcgtgcca taagccgttg cgatcaacgg tatgagcaat ggcatttttg
    11881 cccggttcgg cacgtttcat cgtatcgcgc actggtgcgg ctaggatccc tcccgtgcgg
    11941 ctggtttcgc tcaacgccaa caatcgcgcg aggtcatcct gatgcagaca aggacgagcg
    12001 gcgtcatgca ccaataccca ctgcgcgtcg ccagcggctt tcaaacctgc cagcacggaa
    12061 tcggcacgct catcaccgcc atctacaacg gtgatttgcg gatgattcgc cagaggaagt
    12121 tgtgcaaaac ggctatcgcc aggacttatg gcaatgacga cacgtttcac ccggggatgc
    12181 gccagcagcg catgcaccga gtgttcaaga atggtttgat taccgattga gagatattgc
    12241 ttaggacatt ccgtttgcat tcgacggcca aatccggccg ccggaaccac ggcgcaaaca
    12301 tccaaatgag tggttgccat gttaattccc gggctgattt atcgattgtt ttgccccgca
    12361 gactgtgcgc gcttcgacgc gtcaggcacc agacgataaa aagtttcgcc cggcctggtc
    12421 atgctgagtt cattacgcgc acgctcttcg agcgcctcct ggccgccatt gagatcgtca
    12481 atttcggcaa aaagttgatc gtttcgcgct ttaagtttcg cgtttgtagc ttgctgtgcc
    12541 gccacatcat cattgacgcg ggtatagtca tgtataccgt tcttaccgaa ccacagcgaa
    12601 tactgtagcc agaccagaat agccagcaac agcagcgtta gtttacccat cctgccccct
    12661 gaaaaacggc atcatcatcc catgcatccg aagacgactc tacatcctct gttggggata
    12721 ccgcgacaac gcgggcaaat gtaccacatt tgtccattgt tacgtatacc cagggcgtgc
    12781 agaacataat ctcattatta gttacggttt gaattatgaa cagaggagac aagaaagtac
    12841 aaattagccc agtagccaca taaacagtgc gccaaacata atgcctactg tcaccagggt
    12901 gaaaacaata ctgtagcgta gctttccgtc catcaatgaa tgcagcgcaa tccccaccac
    12961 taccgcgacg ggcatcagcg ccagaaagaa aggccaggtg tagataaaga agaacagcgt
    13021 gttagagcca taaatcaaca tcggcatcgc cagcgcaaat aaccaggaga taaaaccgac
    13081 cacggcacca ggcagtgacc atgtggtttc ttcatcctca gtaaggctgt cgttatttgt
    13141 tagtgtaatg ttatggctat tacgcatatt tgatcctgtt actttgacga accgggcatg
    13201 gaaacccggt ggtgtctcag gatctgataa tatcgttctg tctcaacaga tctaataatt
    13261 gctgtaccaa atttgttact aattgttcac cattgagatg aatttctgcc gattcaggcg
    13321 cttcgtaaac ggaatctatt cccgtaaagt tgcgcagttc accggcacgc gctttcttat
    13381 ataagccttt tggatcgcgg gcttcgcaaa tcgccagcgg cgtatcgaca aacacttcga
    13441 taaagcgccc ttctcctacg cgttcacgaa ccatctggcg ttcggcgcgg tgtggcgaga
    13501 taaatgcggt cagcaccacc agtccggctt caaccatcaa attcgccact tcaccgacgc
    13561 gacggatatt ctctttacga tcggcatcgc taaaaccgag atcgctgcat aatccgtggc
    13621 gaacattatc gccatccagc agatacgtac tgacgccgag tttatgtaac gcctcctcca
    13681 gcgccccggc gaccgttgat ttaccggacc cggagaggcc ggtaaaccac agcactacac
    13741 cacgatgacc gtggtgtagc tcgcgttgtt gcacagtgac cggatggcta tgccagacga
    13801 cgttttcgtc atgcagcgcc attatttatc ccccagcaaa tcgcgcgcgc cccagtgcgg
    13861 aaagtggcga cgaaccagag cattcaattc cagttcgaat gcactgaatt cagatggcgc
    13921 agcagttgcc tggctaactg gctcgtgcac cataccggca cccacggtca cattgctcag
    13981 gcgatcgata aaaatcagcc cacccgtcac cggattttgt tgataacgat ctaacaccag
    14041 cggctcgtca aaagtgagat ccacgaggcc gatcccattc agtggcaggt tttcaacttc
    14101 acgctgggta aggttattaa tatcaacctg atagcgaatg ccatcaacac gcgcgcgcgt
    14161 cttcttaccg gcaattttga tgtcgtaact ctgccctgga gaaagcggct gttccgccat
    14221 ccataccaca tccaccgacg cgctctgcac cgccggtaac gcttcgtctg ccgccagcag
    14281 cagatcgcca cggctgatgt cgatctcatc cgtcagcacc agggtgatcg cttctccggc
    14341 aaaggcttct tcgcgatcac catcaaaagt cacgatccgc gcgacgtttg attccacacc
    14401 agagggcagc acttttacac gttgcccgac ttccacgcga ccggatgcca gcgttccggc
    14461 gtagccacga aaatcgagat tcgggcgatt aacgtactgc accgggaagc gcattggctg
    14521 agcatccacc actcgctgga tctccacggt ttccagcact tcgagcagtg tcggaccgct
    14581 gtaccacggc atactttcac tttgcgatgc cacgttgtcg ccttccagtg cagagagcgg
    14641 cacaaagcgg atatccagat tacccggcag ctgcccggca aaggtcagat aatcttcacg
    14701 aatacgggtg aacgtctctt cactgtaatc caccagatcc attttgttga tcgccacgac
    14761 cagatgtttg atccccaaca gtgtggagat aaaactgtga cgacgggttt gatcgagcac
    14821 gcctttacgg gcatcgatca gtaagatcgc cagttcacat gtcgatgcgc cagtcgccat
    14881 attgcgagtg tactgctcgt gccctggggt gtcggcgata ataaatttac gcttctcggt
    14941 agagaaatag cggtaggcca catcaatagt gatgccctgt tcgcgctcag cttgcaggcc
    15001 gtccaccagc agagccagat ccagcttttc gccctgggta ccgtgacgct tactgtcgtt
    15061 atgcagcgat gagagctgat cttcatagat ttggcgagta tcgtgcagca ggcgaccaat
    15121 cagagtgctt ttgccgtcat cgacgctgcc acaggtcaga aaacgcagca ggcttttatg
    15181 ttgttgcgca atcatccagg cttcgacgcc gccttcattg gcgatttgtt gtgcaagtgc
    15241 ggtgttcatc ttaaaaatac ccctgacgtt ttttcagctc catagatccc gcctggtcgc
    15301 ggtcaatcac gcggccctga cgttcactgg tggtggaaac cagcatctct tcgatgattt
    15361 ccggcagtgt ttgtgcattt gactccaccg caccggtcag cggccagcag cccagcgtac
    15421 ggaaacgcac catccgtttt ttaatcactt cgcccggttg caggtcgata cggttgtcat
    15481 caatcatcat caacataccg tcgcgttcca gaaccggacg ttccgcagcg agatacagcg
    15541 gcacaatgtc gatattttcc agccagatgt attgccagat atcctgctcg gtccagttag
    15601 agagtgggaa gacgcggatg ctttcgcctt tgttaatttg cccgttgtag ttgtgccaca
    15661 gctccgggcg ctgatttttc ggatcccagc gatggaagcg gtcacggaaa gagtagattc
    15721 gctctttagc gcgggatttc tcttcgtcac ggcgcgcacc accgaaggcg gcatcaaaac
    15781 cgtatttgtt cagcgcctgt ttcaggcctt cagttttcat aatatcggta tgtttcgcgc
    15841 tgccgtgcac gaatggatta atccccatcg ccacgccttc cgggttttta tgcaccagca
    15901 gctcgcagcc gtaggctttc gccgtacgat cgcggaactc atacatctcg cggaatttcc
    15961 agccggtatc gacatgcagc aacgggaaag gcagcgtacc tggataaaac gccttgcgcg
    16021 ccaaatgcag catgacgctg gaatctttac cgatagagta gagcatcacc ggatttgaga
    16081 attctgccgc cacctcgcga ataatgtgga tgctttccgc ctccagttgc cgcaggtgag
    16141 taagtcgtat ttgatccata accgttcctt tgcaataccg ctattttctt gccatcagat
    16201 gtttcgacta tagggagcgt aagagaacga atgaaattac caattagaat gagtagttcc
    16261 ttaacggaat aacgatttgg caaagctaat atcaaaaagt gcttaaggca ccggatttct
    16321 ggcgtttagg aagatttgaa attgttttag cgcagcggaa gtttcatact atggcggtaa
    16381 aaaaatttgc atggtattta aggactcact atgttttccg cattgcgcca ccgtaccgct
    16441 gccctggcgc tcggcgtatg ctttattctc cccgtacacg cctcgtcacc taaacctggc
    16501 gattttgcta atactcaggc acgacatatt gctactttct ttccgggacg catgaccgga
    16561 actcctgcag aaatgttatc tgccgattat attcgccaac agtttcagca aatgggttat
    16621 cgcagtgata ttcggacatt taatagtcgg tatatttata ctgcccgcga taatcgtaag
    16681 agctggcata acgtgacggg aagtacggtg attgccgctc atgaaggcaa agcgccgcag
    16741 cagatcatca ttatggcgca tctggatact tacgccccgc tgagcgatgc tgacgccgat
    16801 gccaatctcg gcgggctgac gttacaagga atggatgata acgccgcagg tttaggtgtc
    16861 atgctggaac tggcagaacg cctgaaaaat acgcctaccg agtatggtat tcgatttgtg
    16921 gcgaccagcg gcgaagagga aggaaaatta ggcgctgaga atttactcaa gcggatgagt
    16981 gacaccgaaa agaaaaatac gctgctggtg attaatctcg ataacttaat tgttggcgat
    17041 aaattgtatt tcaacagcgg tgtaaaaacc cctgaagcag taaggaaatt aacgcgcgac
    17101 agggcgctgg caattgcgcg cagtcacgga atagccgcaa cgaccaatcc gggtttgaat
    17161 aaaaattatc cgaaaggcac tgggtgttgt aatgacgcag aaatattcga caaagcgggc
    17221 attgctgtac tttcggtgga agcgactaac tggaatcttg ggaataagga tggttatcag
    17281 caacgcgcaa aaacagccgc attccctgcg ggaaatagct ggcatgacgt aagactggat
    17341 aatcaacagc atattgataa ggcacttcct ggaagaatag aacgtcgctg ccgtgacgtt
    17401 atgcggataa tgctacctct ggtgaaggag ttggcgaagg cgtcttgatg ggtttgaaaa
    17461 tgggagctgg gtgttctacc gcaggggcgg ggaattctaa gtgatatcca tcatcgcatc
    17521 cagtgcgccc ggtttatccc cgctggcgcg gggaactctt actgcttggt atgcggaatc
    17581 acaccctgaa cggtttatcc ccgctggcgc ggggaacaca ctaagcatac ataactgttt
    17641 ttaaacaaat ttattccgca tcaacaatct accaactaaa ttaaaacatt tccttatttt
    17701 taaagaacgg ataacttatt gattatcaac aggaagaaaa gaaaccaaac gtaacccatc
    17761 caaatccacc ggaatacgtc tgttttctcc ccaggtctga aattcaaaac ccgactcgat
    17821 attggtcgcc caggccatca ccacatttcc gcaaccagcc agttgggtaa tttgctgcca
    17881 gatcatctcc cgaatacgtt ttgatgtatc accaacatac acaccggcac gcacttccag
    17941 tagccagatt gcgagccgtc cacgtaagcg cggagggaca ttttctgtaa caaccacgac
    18001 catgctcatc cgccgcgccc ccggtgacca ctatcaccca gcgtttcagg ttcagggatg
    18061 gcaggcggta acatatccgg cgcgggttgt ggtggttcaa tttcacctgc agcaaggact
    18121 tcctcaatta acggtattaa tttgcccgtt aacttagtgc tacggaaaat atcgcgacag
    18181 gctaatctga cttctttatc aggttctgcg ggttgcctcg ctgctatttc aaatgccttt
    18241 ggcacaaccg aatcaaattt aatgatatcg gctatgtcat aaacaaatga aagcggtttg
    18301 ccactatgga taaacccaat agcgggcgca tatcccgcgg ctaatactgc cgcttcagat
    18361 ataccgtaca gacatgatgt agcagcactg atgcagcgat tcacaacatc gcctttttcc
    18421 cagtctttag gatcgtattt gcgaccattc catttcacac catattgttt cgccagtaat
    18481 gcatacgtct ggcgaacgcg agatccctca attccacgta actgctccac tgaacggcga
    18541 gctggcggtg gttcacgaaa acgtaattca tacattttgc gcaccacctt caaccgtaga
    18601 tcttccgtta aagccagctt tgcctagtag agtaatttat ctgcccgcgc ccctccgggt
    18661 tgtccggagg agtaaacgcg aacgcccgct tcaccgaccc agaccagcag tgttcccacc
    18721 gtggcggcca gatggactgc cgcgtgggaa actcttgttc ccggttcgag cataatgcag
    18781 gcgaccgatc ccactggaat atgcgtgcgg atcccggttt tgtcgatcag cacgaaagcg
    18841 ccgtccagta cgtcgatttg accgtactgg aggaagatca tggaagtgcg atcttttaat
    18901 gggatcgggc tcagcggtac aaacgtcacg cctctgctcc gggtttgatc agcatcagac
    18961 cacaaccgaa cgcccggctt ttgccatacc cctgactgag acgttgcaga aataacccag
    19021 gagcggtgac cgtaagcatc cccgtataat ccacgctact gaactggatc agttgccggg
    19081 agttttcccg ccgcagttgc tgttgtcggt aggcatcaac cgaagtatcc agcagtgtaa
    19141 aaccgcttct ctccccctgc gctgccagcc agtccagcgc agcctgttgt tgatgcaacc
    19201 agacatcact tccttccacc tgccccctca cctgccgttt cgcctccatc agcaagtcgt
    19261 ggcgtttgcc cgctttacaa attgttggat tcgcccgcag gttgaaacaa agggattgtc
    19321 cggtacgcaa ctctggcgca aatggtcggc attcgatggt gaaaatatca ctttccgccg
    19381 ggcgttcctg cgacaggaca aaaaagc
//
LOCUS       DAJTZL010000073        19192 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.073, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000073 DAJTZL010000000
VERSION     DAJTZL010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19192
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.073"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(29..829)
                     /locus_tag="OO544_002204"
                     /pseudo
     CDS             complement(29..829)
                     /locus_tag="OO544_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020218947.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
     gene            1396..1761
                     /locus_tag="OO544_002205"
     CDS             1396..1761
                     /locus_tag="OO544_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCR6767792.1"
                     /translation="MEKCDFYHIIVLSLNFPGYLKMEYGSTKMEERLSRSPGGKLALW
                     AFYTWCGYFVWAMARYIWVMSRIPDAPVSGFESDLGSTAGKWLGALVGFLFMALVGAL
                     LGSIAWYTRPRPARSRRYE"
     gene            1801..2457
                     /locus_tag="OO544_002206"
     CDS             1801..2457
                     /locus_tag="OO544_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HCR6767793.1"
                     /translation="MDLQHWQAQFENWLKNHHQHQDAAQKLAADDDVDMLVILTACYF
                     HDIVSLAKNHPQRQRSSILAAEETRRLLREEFEQFPAEKIEAVCHAIAAHSFSAQIAP
                     LTTEAKIVQDADRLEALGAIGLARVFAVSGALGVALFDGEDPFAQHRPLDDKRYALDH
                     FQTKLLKLPQTMQTARGKQLAQHNAHFLVEFMAKLSAELAGENEGVDHKVIDAFSPAG
                     "
     gene            2524..3942
                     /gene="dcm"
                     /locus_tag="OO544_002207"
     CDS             2524..3942
                     /gene="dcm"
                     /locus_tag="OO544_002207"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HCR6767794.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPSAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHKEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDQGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            3923..4393
                     /gene="vsr"
                     /locus_tag="OO544_002208"
     CDS             3923..4393
                     /gene="vsr"
                     /locus_tag="OO544_002208"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HCR6767795.1"
                     /translation="MADVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGSPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRGREKLTDEALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(4382..5303)
                     /gene="yedA"
                     /locus_tag="OO544_002209"
                     /pseudo
     CDS             complement(4382..5303)
                     /gene="yedA"
                     /locus_tag="OO544_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
     gene            5476..6393
                     /locus_tag="OO544_002210"
     CDS             5476..6393
                     /locus_tag="OO544_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HCR6767796.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            6472..6654
                     /locus_tag="OO544_002211"
     CDS             6472..6654
                     /locus_tag="OO544_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HCR6767797.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETELVPGEGSRSAEEV"
     gene            6825..8519
                     /gene="yedQ"
                     /locus_tag="OO544_002212"
     CDS             6825..8519
                     /gene="yedQ"
                     /locus_tag="OO544_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator YedQ"
                     /protein_id="HCR6767798.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHVVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRNAV
                     TEFEQHRDEHAWQIELNRRRTLSVNGVSDALVSEGNLLSRENESLDNEITAALEVGYL
                     LRLAHNTSSMVEQAMYVSRAGFYVSTQPTLFTRNVPTRYYGYVTQPWFIGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSNNYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLRFLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGIRMDSRYVSWERL
                     DHFDGVLVRVHTLSEGVRGDFGSISIALTLLWALFTTMLLISWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKARPLAKLCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGASLTEAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVCASDNA"
     gene            complement(8516..9331)
                     /locus_tag="OO544_002213"
     CDS             complement(8516..9331)
                     /locus_tag="OO544_002213"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HCR6767799.1"
                     /translation="MFSIQQPLLVFSDLDGTLLDSHSYDWQPTALWLSRLREANVPVI
                     LCSSKTSAEMLYLQKTLGLQGLPLIAENGAVIQLAEQWQDIDGFPRIISGISHGEISQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FTARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQLSGKRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPTRVWRTQREGPEGWREGLDHFFSAR"
     gene            complement(9629..9856)
                     /gene="yodD"
                     /locus_tag="OO544_002214"
     CDS             complement(9629..9856)
                     /gene="yodD"
                     /locus_tag="OO544_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HCR6767800.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            10019..10207
                     /gene="dsrB"
                     /locus_tag="OO544_002215"
     CDS             10019..10207
                     /gene="dsrB"
                     /locus_tag="OO544_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HCR6767801.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            complement(10251..10874)
                     /gene="rcsA"
                     /locus_tag="OO544_002216"
     CDS             complement(10251..10874)
                     /gene="rcsA"
                     /locus_tag="OO544_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HCR6767802.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQHIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKKLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(11159..11944)
                     /gene="fliR"
                     /locus_tag="OO544_002217"
     CDS             complement(11159..11944)
                     /gene="fliR"
                     /locus_tag="OO544_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliR"
                     /protein_id="HCR6767803.1"
                     /translation="MMQVTSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFVIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPASHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEMFNLLADIISELPLI"
     gene            complement(11953..12222)
                     /gene="fliQ"
                     /locus_tag="OO544_002218"
     CDS             complement(11953..12222)
                     /gene="fliQ"
                     /locus_tag="OO544_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HCR6767804.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            complement(12232..12969)
                     /gene="fliP"
                     /locus_tag="OO544_002219"
     CDS             complement(12232..12969)
                     /gene="fliP"
                     /locus_tag="OO544_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HCR6767805.1"
                     /translation="MRRLLSVAPVLLWLVTPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     SVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            complement(12969..13334)
                     /gene="fliO"
                     /locus_tag="OO544_002220"
     CDS             complement(12969..13334)
                     /gene="fliO"
                     /locus_tag="OO544_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliO"
                     /protein_id="HCR6767806.1"
                     /translation="MNNHATVQSSTPVSAAPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRSGRS"
     gene            complement(13337..13750)
                     /gene="fliN"
                     /locus_tag="OO544_002221"
     CDS             complement(13337..13750)
                     /gene="fliN"
                     /locus_tag="OO544_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HCR6767807.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSSKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLISQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            complement(13747..14751)
                     /gene="fliM"
                     /locus_tag="OO544_002222"
     CDS             complement(13747..14751)
                     /gene="fliM"
                     /locus_tag="OO544_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HCR6767808.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGNGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDGIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            complement(14756..15220)
                     /gene="fliL"
                     /locus_tag="OO544_002223"
     CDS             complement(14756..15220)
                     /gene="fliL"
                     /locus_tag="OO544_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HCR6767809.1"
                     /translation="MTDYAISKKSKRSLWLPILVFITLAACASAGYSYWHSHQVAAED
                     KAQHRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVMYTAFILR"
     gene            complement(15325..16452)
                     /gene="fliK"
                     /locus_tag="OO544_002224"
     CDS             complement(15325..16452)
                     /gene="fliK"
                     /locus_tag="OO544_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook length control protein FliK"
                     /protein_id="HCR6767810.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLALLSEALASETTTD
                     KAAPQVLVATDKPTTKGELLISDIVSDAQQADLLIPVDETLPVINVEQSTSTPLTTAH
                     TMTLAAVADKNTTKDEKADDLNEDLTASLSALFAMLPGFDNTPKVTDAPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTAAASPLITPH
                     QTQPLLTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMVSPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTVNHEPLAGEEDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            complement(16449..16892)
                     /gene="fliJ"
                     /locus_tag="OO544_002225"
     CDS             complement(16449..16892)
                     /gene="fliJ"
                     /locus_tag="OO544_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FliJ"
                     /protein_id="HCR6767811.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            complement(16911..18284)
                     /gene="fliI"
                     /locus_tag="OO544_002226"
     CDS             complement(16911..18284)
                     /gene="fliI"
                     /locus_tag="OO544_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HCR6767812.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPSPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            complement(18284..18970)
                     /gene="fliH"
                     /locus_tag="OO544_002227"
     CDS             complement(18284..18970)
                     /gene="fliH"
                     /locus_tag="OO544_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HCR6767813.1"
                     /translation="MSDNLPWKTWTPDDLAPPQAEFVPMVEPEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKSQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            complement(18963..>19192)
                     /gene="fliG"
                     /locus_tag="OO544_002228"
     CDS             complement(18963..>19192)
                     /gene="fliG"
                     /locus_tag="OO544_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="One of three proteins involved in switching the
                     direction of the flagellar rotation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HCR6767814.1"
                     /translation="ESLLIALKGAEQPLREKFLRNMSQRAADILRDDLANRGPVRLSQ
                     VENEQKAILLIVRRLAETGEMVIGSGEDTYV"
BASE COUNT         4694 a         4825 c         5003 g         4670 t
ORIGIN      
        1 actaaatcag taagttggca gcatcaccca ttgcaagata aacattgttg gcgtcgtatt
       61 ttgcaccaaa tgtccatgct tcagctgtat caccaccagc aaatttgctg tattcattgt
      121 agtgataccc gccaagagct acttgagaat tggtacggtc agagtttgag taagcggcac
      181 ctaagctcaa accgaagtca aagtcatatg aagtggaaag accaaaacca tcaccgttct
      241 ggaagcgaac gtcttcgtga ccatttttag taccttcttg atcttctcct gcattgctgc
      301 cttcgttgtt accttgatac tgcaatgcaa agttcagacc ttccaccaga ccgaagaaat
      361 cactattgcg gtaagtagct accccatttg cacggcctgc catgaagtta tcagtccagg
      421 tataggagtc gccgccgaat acaggaagca tatctgtcca ggcttcaacg tcgtaaatca
      481 caccatagtt acgaccatag tcaaaggagc ccatctcgcc gaacttcaga cctgcaaaag
      541 caagtttgac ataagtatta tcagaacctt cagtgtcgct agcctttgtt ttggtcagtg
      601 cttgaccata accagttaac tgatctgtaa tttgggtctc acctttgaag cctaactcga
      661 tgatagtttc atcgccgtca tcgccagggt tatcggaaaa agtatgacga gcatcaactt
      721 ttccgtacag atccagttta ttgccatttt tattataaat ttcagccgca tttgctgcgc
      781 cagcaactaa taacgctggg accagcattg ccagaacttt tcttttcatt atgtattccc
      841 ttgtgattat aatcttcatg aatatatcaa taagtgccgt tatccaaaaa aaaagcacat
      901 ttggatacta ttctatgaag ttcattttat tttaaagatt gcgtgtaaca aatgtattta
      961 aaagatttca aaatgtttct tatctatttg atataattca gaattatgaa ttctaaatat
     1021 aaacatccat gaaaattata aatgtatatc aatatgttat gagttgaatg gaaagatagg
     1081 gagtatcaat gattgttgcg ccaaacggca ggcgtgatca cgcgcacatt aatgaaggta
     1141 taaaaaaagc cggcatcatg ccggcccaaa aaatataaac aagacgataa aaggctaata
     1201 acggggttat catgacacag aaatcaaaaa aggtataatt gtattcagtg ctaatatatt
     1261 gtttgggtga attaataaaa ctattaccaa ggatataaat aaatctttat tgctgttttt
     1321 ccagtaatta ttgccgttgc gcaaaattta tctatttgtt caaaaaatga ttgagtcttg
     1381 actggctcat ccaatgtgga aaaatgtgac ttttatcaca taatagtact aagtctgaat
     1441 tttccgggtt atctcaaaat ggaatacggt tcgacaaaga tggaagagag actctctcgc
     1501 agccctggcg gcaaattggc tttgtgggct ttctatacat ggtgtggcta tttcgtctgg
     1561 gcaatggcgc gttacatctg ggtgatgagc cggatccctg atgccccggt gtcgggattt
     1621 gaaagcgatt taggctctac cgcgggaaag tggttagggg cactggtcgg atttttattt
     1681 atggctctgg tcggggcatt gttaggcagc attgcctggt acacccgacc tcgtcccgca
     1741 cgtagcagac gctatgaata aaaactgcta aggtggcgac tctttggcaa cggagcgcgt
     1801 atggacttac aacactggca ggcacagttc gaaaactggt tgaaaaacca tcatcagcac
     1861 caggacgcgg cacagaagct ggcggcagat gacgatgtcg atatgctggt gattttaacc
     1921 gcctgttatt ttcacgatat tgtgagcctg gcgaaaaatc atccgcaaag gcagcgttct
     1981 tcaatcctgg cggcagaaga gacgcgtcgc ctgctccgtg aagagttcga gcaatttccg
     2041 gcggagaaaa tcgaggccgt ttgtcatgcc attgccgctc acagtttcag cgcgcaaatc
     2101 gcccccttaa caacggaagc taaaatcgtc caggatgcgg atcggctgga agccctgggg
     2161 gcgattggcc tggcgcgcgt gtttgccgtt tcaggggcat tgggcgtggc gctgtttgat
     2221 ggtgaagatc cgttcgcaca gcatcgcccg cttgatgata aacgctacgc gctggatcat
     2281 ttccagacta agttgctgaa gctaccgcaa accatgcaaa ccgcaagagg caagcagctg
     2341 gcgcagcaca acgcgcattt tttagtcgag tttatggcga agctcagtgc cgaactggcg
     2401 ggggagaatg aaggtgtcga tcacaaggtg atagatgcgt tttcacccgc gggataagcg
     2461 cgtggcccta aatggctgta attatgttaa cctgtcggcc atctcagatg gccggttaaa
     2521 tctatgcagg aaaatatatc agtaaccgat tcatacagca ccgggaatgc cgcacaggca
     2581 atgctggaga aactgctgca aatttatgat gttaaaacgc tggtggcgca gcttaatggt
     2641 gtgggtgaga atcactggag cgcggcaatt ttaaaacgtg cgctggcgaa tgactcggca
     2701 tggcaccgtt taagtgagaa agagttcgcc catctgcaaa cgttgttacc caaaccatcg
     2761 gcacatcatc cgcattatgc gtttcgcttt atcgatctat ttgccggaat tggcggcatc
     2821 cgtcgcggtt ttgaatcgat tggcggacaa tgcgtgttta ccagcgaatg gaacaaacat
     2881 gcggtacgca cttataaagc caaccattat tgcgatccgg cgacgcatca ttttaatgaa
     2941 gatatccgcg acatcaccct cagccataaa gaaggcgtga gtgatgaggc ggcggcggaa
     3001 catattcgtc aacacattcc tgaacacgat gttttactgg ccggtttccc ttgtcagcca
     3061 ttttcgctgg ctggcgtatc gaaaaagaac tcgctcgggc gggcgcacgg ttttgcctgc
     3121 gatacccagg gcacgctgtt ttttgatgtg gtacgcatta tcgacgcgcg tcgtccggcg
     3181 atgtttgtgc tcgaaaacgt caaaaacctg aaaagtcacg accagggtaa aacgttccgc
     3241 atcatcatgc agacgctgga cgaactgggc tatgacgtgg ctgatgcaga agataacggg
     3301 ccggacgatc cgaaaatcat cgatggtaaa cattttctgc cgcagcaccg tgaacgcatc
     3361 gtgctggtgg gttttcgtcg cgatcttaat ctgaaagccg attttactct gcgtgatatc
     3421 agcgaatgtt tccctgcaca gcgagtgacg ctggcgcagc tgttggaccc gatggtcgag
     3481 gcgaaatata tcctgacgcc ggtgctgtgg aagtacctct atcgatatgc gaaaaaacat
     3541 caggcgcgcg gtaacggctt cggttatgga atggtttatc cgaacaatcc gcaaagcgtc
     3601 acgcgtacgc tgtctgcgcg ttattacaaa gatggcgcgg aaattttaat cgatcgcggc
     3661 tgggatatgg ccacgggtga gaaagatttt gacgatccgc tgaatcagca acatcgtcca
     3721 cgtcggttaa cgcctcggga atgcgcgcgc ttaatgggtt ttgaagcgcc gggagaagcg
     3781 aaattccgca ttccggtttc ggacactcag gcctatcgcc agttcggtaa ctcggtggtc
     3841 gtgccggtct ttgccgcggt ggcaaaactg cttgagccaa aaatcaaaca ggcggtggcg
     3901 ttgcgtcagc aagaggcaca acatggccga cgttcacgat aaggccactc gcagcaaaaa
     3961 tatgcgcgcg attgccacgc gtgatacggc gattgagaag cgcctcgcca gtctgttaac
     4021 cgggcagggc ctggcgtttc gcgttcagga cgccagtctg cccggaagcc cggattttgt
     4081 cgttgatgaa tatcgctgcg tgatatttac ccatggctgc ttctggcatc atcatcactg
     4141 ctatctgttt aaagtgcctg cgactcgaac cgagttctgg ctggagaaga taggtaaaaa
     4201 tgttgaacgc gatcgccgcg atatcagtcg tttgcaggaa ctcggctggc gcgtactgat
     4261 tgtctgggag tgtgcattac gtgggcgcga gaagctgacg gatgaagcgc ttaccgagcg
     4321 tctggaagag tggatctgcg gcgaaggtgc cagcgcgcag atcgacacgc aggggattca
     4381 tttactcgct tgatgcgtcc tgaataactg gcgcaactac gggttttgcc gggaagagat
     4441 attttcccaa cgtgaccagt accaccgcga agacaattac gccgagcgcc agccattcaa
     4501 tcttcgacag cgtctctccg cccagtcccg tacccagcaa gaccgcgacc accgggttaa
     4561 cgtaggcgta gctggtggcg agagccggac tgacattacg gattaaatac atataagcgt
     4621 tgatggcgat aatcgaacca aacagcgcca gatagccgac cgcaaggaag cctgaaaggg
     4681 aagggagcgc cgtcagtttt tcacctgcaa tcatcgacgc gatcattaac accacgcctg
     4741 ccgccagcat ctcaatcgca cccgccatca tccctacagg taaggtaatg cgcgagccat
     4801 aaaccgagcc aaacgcccag ctaatcgagc cgattaaaat cagaatcgcg ccccacggat
     4861 tgccgcttaa atttccaccg ctattgagca tgatgattcc ggcaagccca atggcgatac
     4921 ccacccattc cagtttgcgc gttttaatgc caaacaggcg gctgaagcac cagggtaaag
     4981 aggggcacgg ttgcaaccac aacggcggcg atgccggaag gaacattttg atgctcggca
     5041 accgtcacca tgccattacc gacagccagc aataacaggc caatcagcgc ggcattgagc
     5101 agcggacgta gcggggggag cttgtgtccg cgcagtagca aaaatgccat caataaaata
     5161 ccggctgcca ggaatcgaac gcctgccatc attaacggag gccagctttc cacgccaatc
     5221 cgaatgacaa aataggttga gccccaaatg atatacaacg caaacagcgc gccaaaaagc
     5281 ggtaacaact ggcggaaacg cataatccct cacggtggaa ataaaaaggt ggttcatagt
     5341 aaacgtgaaa atcattctgc tggcgagaga tataattgca cttgattgta aaaaaaatgt
     5401 tgacctgtga agcaagtatc agagctgccg tttttgcttc atacttacac ccttcaacaa
     5461 taaaaatgag agggaatgct tttggccggg agtagtttac tgacgttgct cgatgatatc
     5521 gccacactac tggacgatat ctccgtgatg ggcaaactgg cggcgaagaa aaccgccggt
     5581 gtattagggg atgacttatc gctcaatgcg caacaagttt caggcgtgcg ggccaaccgg
     5641 gaacttcccg tggtctgggg cgtggcgaaa ggatcgctga ttaataaagt gattctggtg
     5701 ccgctggcgc tgatcatcag tgcgtttatc ccgtgggcga ttacgcctct gttgatgatt
     5761 ggtggcgcgt ttctctgctt tgaaggagta gagaaagtgc tgcatatgct ggaggcgcgt
     5821 aaacataaag aagatccggc gcagagccag cagcgtctgg agaagctggc ggcgcaggat
     5881 ccgctgaagt ttgaaaagga caaaataaaa ggggcgattc gtaccgattt tatattgtct
     5941 gccgaaatcg tcgccatcac gctggggatt gtggccgaag cgccgttgct taatcaggtg
     6001 ctggtgcttt caggcatcgc gctggtagtg accgtgggcg tctacggtct ggtaggggtt
     6061 atcgttaaga ttgatgacct ggggtattgg ctggcggaaa aatccagcgc gctgatgcag
     6121 gcattaggta aagggttatt gatcattgcg ccctggttga tgaaggcgtt atcgattgtc
     6181 ggcacgctgg cgatgttcct cgtcggcggc ggtattgtgg tacatggtat tgcgccgctg
     6241 catcacgcca ttgaacattt cgccgggcag caaagtgcag tggtggcgat gatattaccg
     6301 actgttttaa atctgattct tggatttatc atcggcggca tcgtggtgct gggagtgaaa
     6361 gccgtagcga aaatgcgcgg tcaggcacat taatactcgt acaggctatt atgcaaaatt
     6421 cgccatcttc aactaaggtg aacaagtttc actcgaaaaa aggaggcgag tatgagcttt
     6481 atggttagtg aggaagttac ggttaaagag ggcggcccgc ggatgattgt cactggatac
     6541 tccagcggta tggttgagtg tcgttggtat gacggttacg gggtcaagcg ggaagctttt
     6601 catgaaaccg agcttgttcc gggggagggg agtcgttctg cggaagaagt ttgaaagggt
     6661 atcattaaca ccaggccgga tcacttccgg cctggtgtgc caatgcctgc aatgaatgat
     6721 agtcgcgtct tatcatgcca ccattataaa attatcgctg ttttgcggta cgctatcgcc
     6781 aggttatgcc agaatcataa aaaagcaggt tgggagtcgt cagggtgcag cacgagacaa
     6841 aaatggaaaa ccagagctgg ttgaaaaaac tcgcacgccg cctggggcct ggtcatgtcg
     6901 ttaatctctg ctttatcgtg gtattgcttt tttccacctt gctgacctgg cgtgaagtgg
     6961 tagtgctgga agatgcctat atctccagcc agcgtaatca tctggaaaac gtagccaacg
     7021 cgctcgataa gcatttgcag tataacgtcg acaaactgat ctttttgcgt aatggcatgc
     7081 gcgaagctct cgtagcgcca ctggatttca cttcactgcg taatgctgta accgagttcg
     7141 aacagcatcg cgacgagcac gcctggcaaa ttgaactcaa ccgacgacgc accctgtcag
     7201 tcaatggcgt atcggatgca ttagtcagcg aggggaatct cctgtctcgc gaaaatgaaa
     7261 gcctcgacaa tgaaattacc gctgcactgg aagttggtta cttgctgcga ctggcgcaca
     7321 acacctcgtc gatggttgaa caggcgatgt atgtctcgcg tgccggattt tacgtttcga
     7381 cgcagccgac cttgtttacg cgcaatgtac caacgcgtta ttacggctat gtcacccaac
     7441 cctggtttat cggccattcg caacgagaaa atcgtcaccg cgcggtacgc tggtttactt
     7501 cgcaaccgga gcacgccagc aatactgaac cgcaggttac cgtcagtgtt ccggtagaca
     7561 gtaataacta ctggtatggc gtgctgggga tgagtattcc cgtgcgtacc atgcagcaat
     7621 ttttaagaaa cgccatcgat aaaaacctcg atggtgagta tcagctctat gacagtaagc
     7681 tgagattttt gacctcttcc aatcctgacc atccaacagg gaatattttt gatcctcgtg
     7741 aactggcctt gctggcgcag gcgatggaac atgacacgcg gggcggcatt cgtatggaca
     7801 gtcgctatgt tagctgggaa cgtctggacc atttcgacgg tgtgctggtg cgcgtccata
     7861 cgctaagcga aggcgtgcgc ggcgatttcg gcagtatcag cattgcatta accctgctgt
     7921 gggcgctctt taccaccatg ttactcatct cctggtatgt gattcgccgg atggttagca
     7981 acatgtatgt tctgcaaagc tcgttgcagt ggcaggcgtg gcacgacacc ttaacccgtt
     8041 tatataatcg tggcgcactg tttgaaaaag cccgtccgct cgcgaaattg tgtcagacgc
     8101 accaacatcc tttttctgtc attcaggtcg accttgacca ttttaaagcg attaatgacc
     8161 gctttggtca tcaggcgggc gaccgtgttc tttctcatgc tgccggatta attagcagtt
     8221 ccttgcgtgc gcaggacgtt gccgggcggg tcggtggtga ggagttttgt gtgattctgc
     8281 caggcgcgag tctgacggag gctgcggaag tcgcagaacg tattcgcctg aagttaaatg
     8341 aaaaagagat gttgattgct aagagtacga cgatacgcat cagtgcctcg ttgggggtaa
     8401 gtagcagcga ggaaaccggt gattatgatt ttgaacaact ccagtcactg gctgaccgtc
     8461 ggctttatct cgctaaacag gccgggcgta atcgggtatg cgcgagcgat aacgcttaac
     8521 gagcggagaa aaaatggtcc agcccttcac gccagccttc cggtccttca cgctgcgttc
     8581 gccagacgcg ggtcggatcc tcatcatgca gatgcacccc ttcacggttt agccctttca
     8641 caatcaccgc gtaatccatt acctccagta agggcgcatc gtttggccca tcgcccaggc
     8701 caagtgtggt tgggcgtttg cctgataatt gttgataggt cgcgataatc cagttggcgg
     8761 cctgatcttt tccggcagag gcatccagga cgtgccagaa gcgcgcaccc tgcataaact
     8821 gtaagcccag ttcgttcaga cgagcggtaa attgtgccat acgctcgtca ctgtcgcgcc
     8881 agattagcgt taccgacgcc tcatgcagct gcgtcagcgc cgcctggcta cggcttaatc
     8941 ccgtccattc ggcgatggtt gcatcgtcga catcatcgaa agtcgtgaat ttaaaatgtt
     9001 ctttctcgcg tagcgtattt aaaacctggc tgatttcgcc atggctaata cctgagatga
     9061 tgcgtggaaa accgtctata tcctgccatt gttcagcaag ctggatcact gcgccatttt
     9121 cggcaatcag tggtaaccct tgtagcccca acgttttttg caagtacagc atttccgctg
     9181 aagttttact gctacagaga atgacgggaa cattcgcttc gcgtaaacgg ctgagccaga
     9241 gggcagtcgg ttgccagtca taactatgac tgtccagcag ggtgccatca agatcgctaa
     9301 aaaccagtag tggttgttga attgaaaaca tggtcgtgca gttctcccct taccaggatg
     9361 attctcaaaa cagcacaaat aatcaaagat gattttttcg ggagcgatcc cgttgacgcc
     9421 agagccacca aggggtgacg cacgtcacat ttctattcat aagtagcgtt aattattcat
     9481 atcgcgaata ttttcttgtc agcgaaaaaa attgcggata aggtgatgaa cacatcagat
     9541 ttcctggtgt aacgaatttt ttaagtgctt cttgcttaag caagtttcat cccgaccccc
     9601 tcagggtcgg gattttttta ttgtgcattc aacgattcac ttcagagacg ctgaagtcat
     9661 gaatatccag ttcgaaggca tccgccaatt cacgccatgt atgatattca cgtccatcga
     9721 cgctaacgtg ttcagaatcg ttctggctgc gatgaacttc gctttcgcta atttcattga
     9781 tcgccgccag cagggcatcg acatcgacgc tgacctcacg ttttgcggta tcgctgtact
     9841 cttttgcggt tttcatcatc tttcctcgca accgttactc tgttgttgcc gccagtcgtt
     9901 aagtccgcct ggcatctctt aagtatagac cgtcgagaaa atcagcactc gcgcgcgcct
     9961 ggagctgcca gaaaaacgaa attgttctac actggcacaa agccacagga ggaaaacgat
    10021 gaaggtgaat gatcgggtaa cagtcaaaac ggatggcggt ccgcgtcgtc ctggcgtggt
    10081 actggcagtt gaggagttta gtgaaggcac aatgtacctg gtttcgctgg aagactaccc
    10141 gctcggcatc tggttcttta atgaagcagg gcatcaggac ggtatctttg tggagaaagc
    10201 agagtaatct tattcagcct gactggtggg aaaccaccag tcagaatgtg ttagcgcatg
    10261 ttgacaaaaa taccattagt cacattatcc gtcagtcgga cgacatggta gataacctgt
    10321 ttattatgtg ttttgatctt acgtttaata ttacctttat gcgatgaaac ggtcttggct
    10381 ttgatattca tttggtcaga gatttgaatg gttccctgac ctgccatcca cattcgcaac
    10441 atactcgatt cggttcggct caatgataac gtcggcatat ttaaaaaaga ggttatcgtt
    10501 gtctcttttt tcagaatatc gccaaggata tcgtcgagag attccggttt aatcgattta
    10561 gaacttatca ataatttttt tctgaccaat agatattcat caaaatgaac attggcaatt
    10621 gccataaaaa cgataaataa cgtattggga tgttgattaa tgatgtgctt gatgtgctga
    10681 ctgttagaag catcatggat gaaacagtcc tcattaataa acaccactga agggcgctgt
    10741 gaatcacaag ctatggcaag gtcatcaacg gtttcaatgt cgttgatttc tcttttttta
    10801 acccctctac tcaacagata cccggttaaa cctagtcggg tgtaactaca taaatccata
    10861 ataatcgttg acatggcata ccctcactca atgcgtaacg ataattcccc ttacctgaat
    10921 atttcatcat gactaaacgg aacaacatgg gtcacctaat gcgccactct cgcgattttt
    10981 caggcggact tactatcccg taaagtgttg tataatttgc ctggaattgt cttaaagtaa
    11041 atgttgcgat atgtgagtga gcttaaaaca aatatttcgc cgcaggagta tcctggaaga
    11101 tgttcgtagg tattattttt cggataatcc ttaggataac atgataaacg ttacggaatt
    11161 atattaatgg caattcacta ataatatcag ccagcaaatt aaacatctca ctgaataaat
    11221 gttcgcaaaa aggtgcaatt aacggcatta atgccgccat taaagagata ccgacagtca
    11281 gagttaatgg aaatccaata acaaaaatgg ataattgcgg ggccatacga ttcagtaaac
    11341 ctaatgccag attcagggtc agcagcagag taatgagcgg taacgccagc atcagcccgt
    11401 taaggaaaat caaactcccc gctttggtga gtgccagaaa cgcattgctg ttcaacggtt
    11461 cgccaccaat tggcagggtg tgaaaggtat cgaccagcag tgaaatcaac cataaatgac
    11521 cgttaaatgt caggaacagc agtaacgcca gcatatccat gatacgcgct aaaacgggca
    11581 tattgagatg gctggccgga tcgacaaacg tcgcaaatga cagccccatt tgcagaccga
    11641 taatttcgcc agcggttcgc acagcggcaa aggcaaattg catggtaaaa ccaagcgcaa
    11701 tgccgatcag gatctgctgc acggccagcc acagagcaaa gaacgaaaaa acaggaacat
    11761 cgttggcagg taatgatggg gcaatgacga acgtgatcat cattgccagc cccagtttta
    11821 cccgtttcgg cacgctgcgt tcgctcagaa ttggtgcggt agaaatgagc gccagcacgc
    11881 gcagtaacgg ccagaagtac aggcttaacc aggaaagcca ttgatcgctt gtcacctgca
    11941 tcattgtacg gcctacccga tgatatacgg caggttagtg aacaaggtgc ggacgtaatc
    12001 cagcaacaga ttgagcatcc acggtccggc aataataatg gcaataaata cggcgatgat
    12061 ttttggaata aacgacagcg tcatttcgtt gatctgcgtg gcggcctgca aaatactgat
    12121 gataaggccc gtgaccagcg ccaccagcaa cagcggggca gccagtgcca gcgcgacttt
    12181 catcgcttca gtccccatca tcatgaccga ttcaggtgtc attttgcctc tctagctgta
    12241 aaagctctgc gccagcgaac cgaccagcaa ttgccagcca tccaccagta caaacagcat
    12301 cagtttaaag ggcagagcaa tggtggcggg ggggaccatc atcatcccca atgccatcaa
    12361 cacgctggca atcaccaggt cgataatcaa aaaaggaatg aaaatcgtga agcctatctg
    12421 aaatgcggtt ttcaattcgc tggtcacgta ggccgggagc aaaatgcgca tcggcacggc
    12481 ttcaggtccc tgcaacgggc cggtattcgc tagtctggca aacaacccta aatctgcctc
    12541 acgggtctga cgcagcataa actcacgtag cggctgcgcc cctttttcca gcgcctcctg
    12601 cattgatatt ttctcttcgc tgaatggctg gtacgcatcg acataaattt tgtcgatcac
    12661 cgatgacata ataaaaaagg tcaaaaatag tgccagcccc agcaatacct ggttaggtgg
    12721 cgcggagggc gttcccagcg cgttacgcaa taaaccaaaa acaatgatga tgcgggtgaa
    12781 actggtcatc atcagtaaaa ttgccggaat aaacgtcaac gaggtgatga acaccagcgt
    12841 ctgcaccggg agcgaccagc tttgtccacc gccaggcagc ggctggctgg tgatacccgg
    12901 cagttgcgcg aaggcgaggg gcgtaaccag ccagagaagg acaggtgcga cagacaacaa
    12961 acgacgcatc aggatctccc actacgctta agcaaatttt tcatgaccga ctgaaaatcg
    13021 gtctgcggta tctcttctgt tggtgctgaa gggggaagtt tatgcagcag attgatttgc
    13081 cctgcggtaa cgccgagcac cagccgtgca tcttccacat cgaccaccac aacccgttca
    13141 cgcgcgccca gcgaagcact ggcgctaatt ttcagaccgt taacgccagt gcgtttcggg
    13201 gcaaatccca accgttttac cagccaggca gcagcgagga tcagggcaat aatggcgatg
    13261 agtgcgccgc tgacctgcag cagtggcgca gcagaaaccg gcgtggaaga ttgcacagta
    13321 gcgtggttat tcatcactaa cggctcaggc gacgcattcg ctcagacgga gtaatgatat
    13381 cggtgatccg cacgccatat ttatcggcaa cgaccaccac ttcgccctgg gagattaaat
    13441 aaccgttgat cagaatatcc agtggttcgc ccgccagacc gtccagcgcc acgacggacc
    13501 cttgcgtcag acgcaacagc tctttgatgg tcatccgcgt acggcccagc tcgacggtca
    13561 gcttgaccgg aatatccatt atcaggtcga tatcctgcaa cgttccgctg acatcaccac
    13621 cgccaaattg ctggaacacc gcgtcggcag cgcttttgct gctggttgat ttttgttcgc
    13681 tcaacgcttc agcccacaga tcgtccattg cgccgttgtt gtcatcggcc ggattattca
    13741 tgtcactcat ttgggctgtt cctcgttcag agaatttaaa atcgggttaa tcaaatgttc
    13801 tatccgtaac gcatactgac cgttgagagt gccgtactga ctggtcagca ccgggacgcc
    13861 gtcaacatgg gcgatgatgc catcgggttt ttctatcggc aggacgtcgc cgggtttcag
    13921 ttttaaaatc tgcgacaggc gtagcgagat atcggcaaag ttggcgacca gctccagctg
    13981 tgaatgctgc acctggcgca ccaggttatc gcgccagttc tgatcttcat tacgcgagtt
    14041 ttccagcggc gggttaacca acaattcccg tagcggctcg atcatgctga atggcagaca
    14101 gatattaaat tcgccggtca ggttgccaat ctccacatgg aacggcgtgt taaccacaat
    14161 gtcgttcggc gaggtggtga tattggtaaa tttcacctgc atttccgaac gcacgtactc
    14221 aacttccagc ggattaatcg ccttccaggc gtcgctatag ccttcaagcg ccagctttaa
    14281 catgcggttg atgacgcgct gttcggtatg ggtaaactcg cgaccttcca ctttggtcgg
    14341 gaagcgtcca ttgccgccaa acaggttatc cacggcgata aacaccagac tcggtgaaaa
    14401 caccaccagc cctgtgccgc gcagcggttt cagatggata aggttcaggt tggtcggcac
    14461 cggcaggttg cgggcaaatt catggtacgg ctgaatgcgg atggccccga cggttatatc
    14521 cgggctacga cgcagcaggt tgaacagccc catacgaaaa tggcgggcaa agcgctcatt
    14581 aatgatttcc agcgcctgca aacgttcgcg cacaacccgt cgttgggtat tcggatcgta
    14641 cggacgaatg tcactttcgc cgctaacact ggctgtcggt tcgtctttga cttcgctgtc
    14701 accattcaac agcgcatcaa tttcagcttg agaaagaata ctatcgccca tgtcgttatc
    14761 gcagaataaa agcggtatac atcacgtcgg tgacatcctg tttcggttgc ccggcaacaa
    14821 gcggggtgga aagcgtggtt ttaatctcgg caatcaggtt tttcttgcct tcttctgtcg
    14881 ccagtacggc agcatcctga cgcgaaaaca gtaacagcaa acggctacgg acttccggca
    14941 aatactcact cagccgcgag cgggtagctt catctttgag tcgcagggtt ataccgatat
    15001 aaagcacgcg atccgcatcg cccaaattga ccgtaaaggt atccagcgca tagaagaccg
    15061 gtgagggcac gacgcgatgc tgcgctttgt cttcagcggc aacctgatgc gaatgccagt
    15121 agctgtaacc tgcgctggca caggccgcga gggtaatgaa taccagaatc gggagccaaa
    15181 gcgatcgctt gcttttcttg cttatcgcgt aatcagtcat gtgttgcggt cttcctgtgt
    15241 cgctactgct tatcggctaa ttatcctgcg tcttgagcgc aacaaagcgg ggaaaagacg
    15301 cggattacgg tgctacctct gacgttaggc gaaaatatca acgccgctgt tgcctgttac
    15361 gcgcccttgt aaagagacgg gaaccggaag cgtatcgtct tcttcccccg ccagaggttc
    15421 atggtttact gtgcgttggc tttgctgttg ttgggaagcg gcctgctgct gaccactaaa
    15481 gctttcgcca ctgatgttgc tttgccctaa ctgaatgcca ctctcggcca gctgcgtacg
    15541 cagtaccggc agcgctgctt ccagggcggc gcgtacgtgc tgatgcggcg aaaccatctg
    15601 gatttgcgcc tggttatcat ccactttgag ggagatttgc acttcaccta aatcctgcgg
    15661 gtgcagacgc aactctgcac tttgttgtcc ctggcgggtg aacagcgaaa tatgctggct
    15721 taatgattgt tgccattcgt gagaacccag cggtgcactc aagacaggcg cggcgacggt
    15781 gagcagtggc tgtgtctggt gtggagtgat tagcgggctg gcggcagcgg tcaccggtga
    15841 aggtgtgctg atgacttccg ctttactctg ggcttctgct accagcggtg ttaatggctg
    15901 agctggcgtg ccgggggcat catcaggctg tgctgttgtg agttgcgcag aagtcagttt
    15961 tgtgaagagc gttggtttct ctgccggtaa cacggttgac ggcgcatcag tcactttggg
    16021 cgtattgtca aaacccggca acatcgcaaa aagggcgctc aggcttgcgg tgaggtcttc
    16081 attcagatca tccgcttttt cgtcttttgt cgtatttttg tcagccaccg cagccaacgt
    16141 catcgtatga gcggtggtta acggtgtcga tgtggattgt tcgacgttga tgacaggcag
    16201 tgtttcatcc acagggatca gtaaatccgc ttgttgcgcg tcggaaacaa tatcgctgat
    16261 cagcagctcg ccttttgtcg tgggcttatc tgttgccacc agcacctggg gggccgcttt
    16321 gtcggtagtt gtctcgcttg ctaatgcttc gctcaacaac gcgagaaaat cttgtgcagc
    16381 atcgctggct ttgccgccag gcaatgtggt ggtgtcaacg tcggcggtaa tcaagggcgc
    16441 taagcgaatc attcaggttt cctcatggcg gcgcgctggg cgaactcatc catctttttc
    16501 tgatcgaggc ggttttctgc aagcagtgcc gccgtggatt gccgttcctg cagtgtctgc
    16561 caggcctgca aacgttgttt cttttctcgc cagctgttca gggcaatgtc aactttctgc
    16621 gtccactgat taagttgctg gcgatgctga gtaatggctt tttccagcgt ctggataaac
    16681 tgctgatagt tgatccagcg gttgctggtc atcccggcac tcatatcgct gttgaggtta
    16741 ttgcgatatt cattctgata atcgatcagc attttgagct gttcttccgc ctgctgacac
    16801 ccgcgacgca tttcacccag caggcgcgcg gcatcctcta cctctttttc tgccagatct
    16861 ttcagggtcg ccagcgcacc atgttctgcc atctaccgtt atctcctggg ttatgacact
    16921 gtcgggaaaa tacgctccag cccctggaga gacgcttccc agtccgcacg ttcaaaaatg
    16981 ccttgttgta aatagccctc cagctgcggc cacagggcga tggctttatc gagcatcgga
    17041 tcgctgcctt tggcatacgc gccgacgcta accagatcgc ggttacgctg aaaactcgac
    17101 aacagctgtt tgaaggtgcg cactcgcgcg taatgttgct cactgatcaa cgccgtcatt
    17161 gcgcggctga tcgacgcttc aatatcgata gccggatagt gcccggcttc cgccagtcgg
    17221 cgagacagca caatgtgacc gtcgaggatc gcccgtgcgg agtctgcaat ggggtcctgc
    17281 tggtcatcgc cttcagtaag cacggtataa aacgcggtaa tcgagccgcc gccgctaatg
    17341 ccatttccgg cacgctcgac cagtgccggt aatttggcaa acaccgacgg tggataacct
    17401 ttggtggcgg gtggttcgcc aatcgccagc gcaatttcac gctgggccat cgcgtagcgg
    17461 gtgagggagt ccataatcag caacacatgc tgaccacgat cgcgaaaatc ttcggcaatg
    17521 cgcgtggcat aggcggcacc ttgcattcgc agaagcggag agacatccgc cggagcggca
    17581 atcaccacgg agcgtgcacg cccttcagca ccgaggatat tctcaataaa atcttttact
    17641 tcgcgcccac gttcaccaat caaacccacg acaatgacat cggcgcgggt gtaacgtgcc
    17701 atcatcccca gcagcacact tttaccaacg ccggacccgg caaacagccc catacgctgc
    17761 ccacgcccaa cggtaagcag ggcattgatt gggcgcacgc cggtgtccag cacatgttca
    17821 atcggtgtac gttgcaacgg gttaaatggc ggggtaatca gcgcaccggt ttccgtcgta
    17881 tcgggggagg gcaggccatc gagcggctta ccgctgccgt ccagaacgcg acctaataac
    17941 gccggaccga gcggcaactg cttgccgctt tgcagtcctt cagccgaaat gtttttggca
    18001 taaacccgcg cgccgggcag gacaccttcc acttcctcca gcggcattaa aaacagccgt
    18061 tgaccgttaa agccaacgac ttcgctttct acttcgtgcg tttcgctgcc gttctggcgt
    18121 tcaatgacac aggttgcgcc gagcggcaat tgtaatccgg tggcttccag caccagcccg
    18181 gtagcgcggg ttaatcgccc atagcgacgt accgcaggca actgcgccat tttggcttca
    18241 aagttatcca gcgtggttag ccagcgagtc aggcgcgtgg tcattacacc actcctggtg
    18301 ctgccagacg gcagagttct tgccagcgag tggcgacact ggcgtcgaga tcgccttcat
    18361 cggcggagac tttacagccg ccaggatgga gtgtgggatc gccccgcaag cgccagccat
    18421 gcaaacttaa ggttgcgccg agcatatcat ccacacgttg cagatcatcc gggtgcacgc
    18481 gcagctgtgg tttaccgctg aataacggtt cttgctgcaa caactgttgg atctgtttga
    18541 tcagtgccga gttatccact gttggcgtct gaccgatcac ctgacgtgcc gcctccagcg
    18601 ccatctgcat caggcgcgaa gcaatcacac tatcaagcgc atcaagggta gtttgaaatt
    18661 cgctgaccag ttgctgcatc cgggcatgaa ttggcgcttg ttgagacttc gcctctgcca
    18721 gaccttgctc cagcccctgg gccagtcctt cctgatagcc ctgctcatga ccttgctggc
    18781 gaccttcggc aatccccgcc tgataacctt gctcatgggc ctgcatttgc agttgcgcca
    18841 gttgctgctc aaggctaggt tcggcctctt caatgatggt ttcttccggc tcgaccatgg
    18901 gcacaaactc tgcctgtggt ggcgcgagat cgtccggcgt ccaggttttc cacggcagat
    18961 tatcagacat aggtatcctc gccgctgcca attaccatct cgccagtttc ggcaaggcgg
    19021 cgcacaatca gcagaatcgc tttctgttcg ttttccacct gcgacagacg caccggacca
    19081 cggttggcga gatcgtcgcg cagaatatcg gcggcacgct gcgacatatt gcgcaggaat
    19141 ttctcgcgca gtggctgctc ggcccctttc agcgcgatca acagcgattc gg
//
LOCUS       DAJTZL010000074        19101 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.074, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000074 DAJTZL010000000
VERSION     DAJTZL010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19101
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.074"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            271..837
                     /gene="yqaB"
                     /locus_tag="OO544_002229"
     CDS             271..837
                     /gene="yqaB"
                     /locus_tag="OO544_002229"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HCR6767815.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDIQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVDVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRHYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            834..1262
                     /locus_tag="OO544_002230"
     CDS             834..1262
                     /locus_tag="OO544_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR6767816.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKAIGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            1335..2891
                     /gene="gshA"
                     /locus_tag="OO544_002231"
     CDS             1335..2891
                     /gene="gshA"
                     /locus_tag="OO544_002231"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HCR6767817.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFRDLKRVAQTLDSINGGDAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHT"
     gene            3041..3556
                     /gene="luxS"
                     /locus_tag="OO544_002232"
     CDS             3041..3556
                     /gene="luxS"
                     /locus_tag="OO544_002232"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HCR6767818.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(3620..5158)
                     /gene="emrB"
                     /locus_tag="OO544_002233"
     CDS             complement(3620..5158)
                     /gene="emrB"
                     /locus_tag="OO544_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HCR6767819.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTKRRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(5175..6347)
                     /gene="emrA"
                     /locus_tag="OO544_002234"
     CDS             complement(5175..6347)
                     /gene="emrA"
                     /locus_tag="OO544_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HCR6767820.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            complement(6474..7004)
                     /gene="emrR"
                     /locus_tag="OO544_002235"
     CDS             complement(6474..7004)
                     /gene="emrR"
                     /locus_tag="OO544_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HCR6767821.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            complement(7095..7430)
                     /gene="ygaH"
                     /locus_tag="OO544_002236"
     CDS             complement(7095..7430)
                     /gene="ygaH"
                     /locus_tag="OO544_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HCR6767822.1"
                     /translation="MSYEVLLPGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            complement(7420..8157)
                     /gene="ygaZ"
                     /locus_tag="OO544_002237"
     CDS             complement(7420..8157)
                     /gene="ygaZ"
                     /locus_tag="OO544_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HCR6767823.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            complement(8281..9465)
                     /locus_tag="OO544_002238"
     CDS             complement(8281..9465)
                     /locus_tag="OO544_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6767824.1"
                     /translation="MTKPNHELSPALIVLMSIATGQAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISDKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            complement(9756..10748)
                     /gene="proX"
                     /locus_tag="OO544_002239"
     CDS             complement(9756..10748)
                     /gene="proX"
                     /locus_tag="OO544_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HCR6767825.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYCEGIFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWAEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            complement(10806..11870)
                     /gene="proW"
                     /locus_tag="OO544_002240"
     CDS             complement(10806..11870)
                     /gene="proW"
                     /locus_tag="OO544_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HCR6767826.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPTTAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNSFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            complement(11863..13065)
                     /gene="proV"
                     /locus_tag="OO544_002241"
     CDS             complement(11863..13065)
                     /gene="proV"
                     /locus_tag="OO544_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HCR6767827.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAKLRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            complement(13421..14380)
                     /gene="nrdF"
                     /locus_tag="OO544_002242"
     CDS             complement(13421..14380)
                     /gene="nrdF"
                     /locus_tag="OO544_002242"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HCR6767828.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            complement(14390..16534)
                     /gene="nrdE"
                     /locus_tag="OO544_002243"
     CDS             complement(14390..16534)
                     /gene="nrdE"
                     /locus_tag="OO544_002243"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HCR6767829.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     MATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDATLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPVAGRINMSNLCSEIL
                     QVNCASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYAITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            complement(16507..16917)
                     /gene="nrdI"
                     /locus_tag="OO544_002244"
                     /pseudo
     CDS             complement(16507..16917)
                     /gene="nrdI"
                     /locus_tag="OO544_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
     gene            complement(16914..17159)
                     /gene="nrdH"
                     /locus_tag="OO544_002245"
     CDS             complement(16914..17159)
                     /gene="nrdH"
                     /locus_tag="OO544_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HCR6767830.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            complement(17407..17736)
                     /locus_tag="OO544_002246"
     CDS             complement(17407..17736)
                     /locus_tag="OO544_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HCR6767831.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            17888..18232
                     /locus_tag="OO544_002247"
     CDS             17888..18232
                     /locus_tag="OO544_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgaC family protein"
                     /protein_id="HCR6767832.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            complement(18269..18718)
                     /gene="alaE"
                     /locus_tag="OO544_002248"
     CDS             complement(18269..18718)
                     /gene="alaE"
                     /locus_tag="OO544_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HCR6767833.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSHYQQVKA"
BASE COUNT         4795 a         4814 c         4938 g         4554 t
ORIGIN      
        1 aaccttcagc aatgaaggta tcgaagagat agcggtatta accgcaggca ccaggaagga
       61 taacgttgcg ccagcaacgg cccgaagggc gaacgtcagt gagtcatcct cccggatgca
      121 ccatcttctc caaaaaattt ccttcaaaaa tatctcattt ctaaataact cctttatcca
      181 tttcctgaat tttcttctcc ttcgccatca gcgacaaaat caagcaatta cgctaaagta
      241 gcgtcatatt tttcgacttg cgagaagacc atgtacgagc gttatgcagg tttaattttt
      301 gatatggatg gcacaatcct ggatacggag cctacgcacc gtaaagcgtg gcgcgaagta
      361 ttagggcact acggtcttca gtacgatatt caggcgatga ttgcgcttaa tggatcgccc
      421 acctggcgta ttgctcaggc aattattgag ctgaatcagg ccgatctcga cccgcatgcg
      481 ttagcgcgtg aaaaaacaga agcagtaaga agtatgctgc tggatagcgt cgaaccgctt
      541 cctcttgttg atgtggtgaa aagttggcat ggtcgtcgcc caatggctgt aggaacgggg
      601 agtgaaagcg ccatcgctga ggcattgctg gcgcacctgg gattacgcca ttattttgac
      661 gccgtcgtcg ctgccgatca cgtcaaacac cataaacccg cgccagacac atttttgttg
      721 tgcgcgcagc gtatgggcgt gcaaccgacg cagtgtgtgg tctttgaaga tgccgatttc
      781 ggtattcagg cggcccgtgc cgcaggtatg gacgctgtgg acgttcgctt gctgtgagtg
      841 aagcgttatc gcttttctcg ttgtttgcca gtagttttct cagcgctaca ctattacccg
      901 gcaactccga agtcgtgctg gtggcaatgt tgctttccgg gatcagtcat ccctgggttt
      961 tagttttaac agcaacaatg ggtaatagcc ttggagggtt aactaacgtt atccttgggc
     1021 gtttctttcc attgcgtaaa acatcgcgct ggcaagagaa agctatcggc tggctgaaac
     1081 gctatggtgc agtcacgcta ttattaagct ggatgcccgt ggttggcgat ttactgtgtc
     1141 tgttagcggg atggatgcgc atctcgtggg gaccggtaat cttttttttg tgccttggta
     1201 aagcgttacg ctatgttgca gttgcagcag cgaccgttca gggcatgatg tggtggcact
     1261 aattgtaggc ctgcacatat ggtcaccatt acagttatgc taattaaaac gattttgaca
     1321 ggcgggaggt caatttgatc ccggacgtat cacaggcgct ggcctggctg gaaaaacatc
     1381 ctcaggcgtt aaaggggata cagcgtgggc tggagcgcga aactttgcgt gttaatgctg
     1441 atggcacact ggcaacaaca ggtcatcctg aagcattagg ttccgcactg acgcacaaat
     1501 ggattactac cgattttgcg gaagcattgc tggaattcat tacaccagtg gatggtgata
     1561 ttgaacatat gctgaccttt atgcgcgatc tgcatcgtta tacggcgcgc aatatgggcg
     1621 atgagcggat gtggccgtta agtatgccat gctacatcgc agaaggtcag gacatcgaac
     1681 tggcacagta cggcacttct aacaccggac gctttaaaac gctgtatcgt gaagggctga
     1741 aaaatcgcta cggcgcgctg atgcaaacca tttccggcgt gcactacaat ttctctttgc
     1801 caatggcatt ctggcaagcg aagtgcggtg atatctcggg cgctgatgcc aaagagaaaa
     1861 tttctgcggg ctatttccgc gttatccgca attactatcg tttcggttgg gtcattcctt
     1921 atctgtttgg tgcatctccg gcgatttgtt cttctttcct gcaaggaaaa ccaacgtcgc
     1981 tgccgtttga gaaaaccgag tgcggtatgt attacctgcc gtatgcgacc tctcttcgtt
     2041 tgagcgatct cggctatacc aataaatcgc aaagcaatct tggtattacc ttcaacgatc
     2101 tttacgagta cgtagcgggc cttaaacagg caatcaaaac gccatcggaa gagtacgcga
     2161 agattggtat tgagaaagac ggtaagaggc tgcaaatcaa tagcaacgtg ctgcagattg
     2221 aaaacgaact gtatgcgccg attcgtccaa aacgcgttac ccgcagcggc gagtcacctt
     2281 ctgatgcgct gttacgtggc ggcattgaat atattgaagt gcgttcgctg gacatcaacc
     2341 cgttctcgcc gattggtgta gatgaacagc aggtgcgatt cctcgacctg tttatggtct
     2401 ggtgtgcgct ggctgatgca ccggaaatga gcagtagcga acttgcctgt acgcgcgtta
     2461 actggaaccg ggtgatcctt gaaggtcgca aaccgggcct gacgctgggt atcggctgcg
     2521 aaaccgcgca gttcccgtta ctgcaggtgg gtaaagatct gttccgcgat ctgaaacgcg
     2581 tcgcgcaaac gctggatagc attaacggcg gcgacgcgta tcagaaagtg tgtgatgaac
     2641 tggttgcctg cttcgataat cccgatctga ctttctctgc ccgtatctta aggtctatga
     2701 ttgatactgg tattggcgga acaggcaaag cgtttgcaga agcctaccgt aatctgctgc
     2761 gtgaagagcc gctggaaatt ctgcgcgaag aggattttgt agccgagcgc gaggcatctg
     2821 aacgccgtca gcaggaaatg gaaaccgctg ataccgaacc gtttgcggtg tggctggaaa
     2881 aacacacctg acagaaaaga aaaaggccac tcgtgagtgg ccaaaatttc atctctgaat
     2941 tcagggatga tgataacaaa tgcgcgtctt tcatatactc agactcgcct gggaagaaag
     3001 agttcagaaa atttttaaaa aaattaccgg aggtggctaa atgccgttgt tagatagctt
     3061 cacagtcgat catacccgga tggaagcgcc tgcagttcgg gtggcgaaaa caatgaacac
     3121 cccgcatggc gacgcaatca ccgtgttcga tctgcgcttc tgcgtgccga acaaagaagt
     3181 gatgccagaa agagggatcc ataccctgga gcacctgttt gctggtttta tgcgtaacca
     3241 tcttaacggt aatggtgtag agattatcga tatctcgcca atgggctgcc gcaccggttt
     3301 ttatatgagt ctgattggta cgccagatga gcagcgtgtt gctgatgcct ggaaagcggc
     3361 aatggaagac gtgctgaaag tgcaggatca gaatcagatc ccggaactga acgtctacca
     3421 gtgtggcact taccagatgc actcgttgca ggaagcgcag gatattgcgc gtagcattct
     3481 ggaacgtgac gtacgcatca acagcaacga agaactggca ctgccgaaag agaagttgca
     3541 ggaactgcac atctagtcag taaactatct tcacaattaa ttgaaaaaag ccagttcaaa
     3601 tgaactggct tagttgtact tagtgcgcac cgccgccacc gccacctgcg ccaaatggcg
     3661 gtttagcaaa ccacaccagc cccagcagta cgaggaatat cccggctgac atccagaaga
     3721 tctcattggc ggaaataatc agcccctgat tggtgatctg ctgggcaatc cagcctgatg
     3781 cctgctgttg cgtcatccca agcccttcca gttgactgta catcgcctgg gcattcgggt
     3841 tgaacgggtt taccgactca gtcaactgcg catggtgcat cgactcgcgg ttggtccaca
     3901 tggtcgtggt tatcgacgtg ccgatagacc ccgccagcgt tcgcgtaaag ttagagaggc
     3961 tcgatgccgc cgccagtcgt tccggtggca aaccagacag cgtaatggtg gtcagcggca
     4021 taaagaagca ggccaccgca aacccctgga taaactgcgg ccaggccgac gcgccaaaat
     4081 ccatacctgg ttcaaaggta taggcacgcc agtagaagca gacggcatac ataataaagc
     4141 tgaaggttac cagccgccgc atatccagtt tatgcgcgaa gcggccgata atcggcgaca
     4201 ggatcaccgg aataatccct accggcgcag aggccaaacc tgcccaggtc gccgtgtaac
     4261 cgtagacctc ctgcaacaac tgcggcagca gaacaatagc gccgaagtag agcatatacg
     4321 cgaggctgat acacaagcag ccgatggtga agttgcgcga cttaaacaac gacagatcga
     4381 ctatcgggtt atcgtcggtc agctcccaga caatcaggaa gcagatagcc accaccgcca
     4441 ccacggtaag gatgataatt tcctgtgatg aaaaccagtc cagctcttta ccgcggtcga
     4501 gcataatctg caggctgccg ataccaataa ccagcagtgc cagccccacg gcatcaatcc
     4561 gccgccgttt ggtgcgggtt tcacgtccgc gcagagtttg cagtgtcatc aacaccaccg
     4621 ccacgccaat cggcacgttg atgaagaata tccagcccca gtggtaatta tcgctgatat
     4681 aaccgccgag gatcgggccg caaattggcg cgacaatcac cgtcatcgac cacaacgcca
     4741 gcgcgatcga gcgtttggct ggcgggtagt tattcagcaa tagactttgc gaaagcggga
     4801 tcaacggccc ggcgacaatc ccctgaatca cgcggaagaa gatcagcata ttcaggctgc
     4861 tggagacacc acacgcccac gacgcaatag caaaggcgat ggtggaccaa aggaacagtt
     4921 tcacttcccc gacgcgcttt gccagccagc cggtaagcgg gatcgagatg gcattcgcca
     4981 ccccgaaaga agtgattacc cacgttccct ggctgagcga tgagcccaga ttcccggcga
     5041 tagtggggat cgccacgtta gcaatggtgg agtccagcac ctgcatgaat gtcgccagtg
     5101 acagcgcaat cgtcataatg accagttgcg cgccttccag cggtttttgc tgttgcatca
     5161 cacgcacctc tggattagcc agcgttagct tttacgatat cgtcgatcag tttattgaca
     5221 ggtgccaggc tgatttcacg cgcggtactt accgcgaccg gtgttgaacg tactttattt
     5281 gccagtacct gaccgtcacg gttagtggta ttgacgctca ccagtgtgga caaaccaatg
     5341 cgcagcgggt attgctccag ctgtttctgg tccagttcga tacgcacagg tagacgctga
     5401 acgactttga tccagttacc ggtcgcattt tgcgccggaa gcagtgagaa tgcgctacct
     5461 gtgcccatat ccagaccaac cactttaccg gtgtatttca catcatcgcc gtaaatatcc
     5521 gtggtgatag tgaccggctg accgatacgc atattggcaa tctgcgtctc tttaaagttg
     5581 gcatccaccc acatattggt ggctggaacg accgccatca gcggcgtcgt tgggctaatt
     5641 tgcgccccag gctgtaccgc acggcgggag acataaccgg tcatcggact gacaatacgg
     5701 gtacgctcca gcgccagcca ggcgttacgt acttcggtgg cagcctgttg cacggcaggc
     5761 tgatcttcca gtttagtccc cagaatcatc gcctgattgg cattgtattg ttgaatagcg
     5821 acgtccagtt gcgcctgggc actggtgacg gcgtcgcggg cgtgttgcag ctcttcgcga
     5881 ccaatcaggt tggcattgcc cagcggcaca cggcggttgt agtcgctttg tgcttttgcg
     5941 agggcgattt tctgcacctc aatattcgcc tgcaactgtt tgctgttaat catcagctgg
     6001 tgggtttggc gaacgctgga agccagtgca gttttggctt tttcaaacgc ctggcgagca
     6061 tctgtcgggt cgagagtgac cagcacgtcg ccttctttta caaaatcggt gttatcggcc
     6121 cagactttcg tcacgctgcc agacacctga gacataattt gaatctgatt ccctgccacg
     6181 tatgcgtcat cggtttcttc gaaatgacgc agtaccaaaa accaataaat ccctatcgct
     6241 acggcaataa ttataaagag caaggtgaga aggaggagca gacgcttacg tttgccgctc
     6301 ttctttaccg gttgctgcgg ggtttgagtc tccgcatttg cgctcatatt gttctccacg
     6361 atcttcttaa ctcatcggct gagccgacct gcttgccaaa aaggccagca tgttgcgatg
     6421 ctggccggtc attttttctt ttataaatct ggatttttga gcgagatgac gcgttagctc
     6481 atcgcttcga gaaccacacc gtcttgttcc atctggtcga gacgggagag caatttgcgg
     6541 gtgatttgct cgagctgatc tttttctgtt gtgctgagcg cggaccagag ttgatgcagg
     6601 cagttatgct gcggtggtaa aacctcgcgc aaaaactcgt gacctttttc cgttaattgc
     6661 agatgcaggc agcggcgatc gttatcgctt tcacgacgtt cgatccaacc gcgtttttcc
     6721 agttcatcgg caatacgcgt cgcgttggta cgggatgatc caagagcaca acttaattca
     6781 gaaggctgaa tactgtggtt ttcctgagac tccagcgtaa tcaacgccat aaacaacgtc
     6841 tcgttaatcc cctgagcctt cagcatttta ttgcggttct ccagcagctt gctttgcatg
     6901 tgcatgcaaa gacgagtcag aaggatctcc tgataaggaa aatcttcgtg gcggctggcg
     6961 cgaaatttta gcatttgttc aatgggcgta aacgaactat ccatttgggt atgacctcat
     7021 taatttcagc cgatatagta acgacagtga caaataaagt aaatgtattg ttttagaaaa
     7081 atgattcttg tgggttatat aatcgccatc actttccagg cgagcccata ggccagcgca
     7141 ctaagcagtg ttgggatgat aatgctgcgt gttttataga aactggcacc cagtaccgcg
     7201 aagccgacca gcgtgggcac gaaacggcgt gtatcgtgca tcacttctgg tgcggtagag
     7261 acaaccagca gagcgcatat cgaggcgatg ccaatggtgt cgagcaaaat acctaccgcg
     7321 ccacgtttgg ttgggcgggc attacccaca cgcaggcgca gcggcaaata gcggaagcaa
     7381 taattcgccg cgccaaccag taacccaggc agcagaacct catagctcat cgggcgctcc
     7441 ttgccagaat gcttggatta acgcagtgag gcagccacag acaatgcctg ccagaatggc
     7501 gacgggaata gaaaatagcg ttacgcctgc aagggcacca actaacgctg cggtaacgca
     7561 aagagattgt ttgcgctgga aagaggcgag caggaaactc ataaagagtg ccggaagcat
     7621 aaaacctaat gctgcttcaa cggcgggata accttgcagc aagccgctgc cagagaatgc
     7681 ccctattacc gtaccaaata cccacgatga ccatgaactg aaggcaatgc cgatcatcca
     7741 gttctcgctc cagcggcgat tattgcgtac cagttttgct gttgcggcgg caaaaacctc
     7801 atccgtcagg ccaaacgccc acagggcggt tttcgatttt tgcagacgct gaataatacg
     7861 gctacgcagt gacgggccat acaacacatg gcgaacatcc attgccatga cggtcagtgc
     7921 agcaacccac aaactactcc cggctgccag catcgcggta atgacgaact ggctcgcgcc
     7981 tgcataaatg atgcaggaga aaaaaacgct ttcgagagga gagaatccca gacgggtcgc
     8041 attcagaccg aacgcaaagg ccaccggaat ataactaata acaatcggta aactgtcttt
     8101 gcatccttcc atgaaggtcg ccgaaccagg agcaggctgt ggagtagggc tttccataga
     8161 gtgtacgctt aacgattgtt aatattataa atagactgaa tgaatatctt aaccttatca
     8221 gactgatggg cttcttaaca cccttataag tgtaaagcca cgaaaacggt tgctgattgc
     8281 ttaatttgcg gcttcctgac gatgaaaacc tcgccaccag accagtaagt taactagggc
     8341 aatcgtcgcg ccagccagac aaacgccagc ccaaccgcca tgttgccagg ctgaggctga
     8401 aattagcgaa ccggcggcac cgccaataaa gtagctggtc atgtaacctg cggtcaggcg
     8461 attacgcgca tcagggtgta ttcgataaat taccgtctgg ttagtgatat gcacgccctg
     8521 tacggtgaga tccagcacca ggatgccgat aatcagcgcc agtacggaag tgtgaccaaa
     8581 ccagatcgcc agccatgaaa gtaatagcag cagcagaccg aaagttgtgg tgtggtgcga
     8641 tttaccctta tcggcaaaac cgcccgccgg acgagcgccc aacgctccgg cagctcccgc
     8701 aagtccaaac agaccaatga caccatcgct gtagttaaaa ggtggagcgg caagcaaaaa
     8761 ggccattgag gtccagagaa tgctgaagtt ggcaaaggtc aggcagccca gcaatgcgcg
     8821 ggtgcggagg atcttatcgc tgataaacat gctgaaaacg gaacctaaca actgtgggta
     8881 gttgaggtgg gtttctgatt tcatttgtgg cagaccacgc cataatgcta gcgccatcag
     8941 tgccattaac accgaggcaa cccaaaagac ggtgcgccag ccgccgaggt ttgccagtaa
     9001 cccggcaacg gttcgtgcca gcaagatccc caacagcaga ccgctcataa tggtgccaac
     9061 cactttgccg cgtttatccg gtgaagccag cgtcgctgcc agcggaacca gaatttgtgc
     9121 cacgactgag aataaaccgg ttaatgccgt accgaggatc atcatcgcca gcgactgact
     9181 gctggcagtg attaacatgc cgcctgccgc cagtaaggtc atcgagacaa tcaggcggcg
     9241 gcgttcaaac atatcaccga ggggaacaag aaacagcaga cctgctgcat agcccaactg
     9301 cgcggcggta acaataaagc ctgccgaact ggcggaaagg gaaaagttac gcgcgatggt
     9361 gtcgagcaat ggctgggcgt aatagttact ggctaccgcc tgaccggtgg cgatagacat
     9421 cagcacgatc agcgccgggc taagctcatg attaggttta gtcattattt ttgttcatca
     9481 ggtgatgatt aagctggaaa tcaataatag cggatgttga tgaatggggg cgaaagatag
     9541 cttgttgaaa tgtgcgatgt ggtttgtagg catgataaga cgcgccagcg tcgcatcagg
     9601 cattgtgcac agaacggcgg atgcggcgaa aacgccttat ccgccatacg gaatgagtgt
     9661 attttgtagg catgataaga cgcgccagcg tcgcatcagg cattgtgcac agaatggcgg
     9721 atgaggcaaa aacgacttat ccgcccgaat aaaaattact tctgcgctgc cagcgcctca
     9781 ttcacccagc catcgaactg ctgctggtgg gctttgatcc aaccatcaac atggccctga
     9841 atatcgcctt ctgaggcttt gccgtcatgc ataatggcgt tctgggcgtt aatatctgcc
     9901 actggcaact gcataatggc aaaaagtttc gctgctgccg ggtttttctc ggcccaggct
     9961 ttgttggcaa cgatatgcat ggtgctgacc gggaagccat aattcgcacc attcggcagt
    10021 ttggtatcgg cgtttttatc gcccggcagt gcggagaacg gcacctgcaa ccagaccaca
    10081 tctttccctg gcttcagctc attactcacc cagtacggcg tccaggtgta ataaaacacc
    10141 ggtttgccct ctttgtagcg actgatggtg tcggccatca tcgctgcgta gttcccctga
    10201 ttatgcgtca cggtgttggt cagttcatac gcggcaagct ggtggttgat cgcaccttcg
    10261 cagccccagc ctgggttaca gccggttaaa tccgctttac cgtcgccgtt ggtatcgaac
    10321 agtttggcga tcttcggatc tttcagttgt gcgatgttgg tgattttgta ctggtcggcg
    10381 gttttcttat cgatcaggta accctgtgcc gcgccgttaa cgaatatccc ttcacaataa
    10441 aatttcttat cgccaccggc agcttcgtac atgttgtcat gcagcggcgt ccagttcacg
    10501 gcggtgaagg ttgcatcgcc ggaagcaagt gaggtgtagc caacgttgta atccacttcg
    10561 ctgggtttat tgacggtata acctaatttc tccagtgcac gactgaccag cagcgtctgg
    10621 aaggtttctt cagtgatggt gctctgaact ggattaacag taatgccttt gcccggcaga
    10681 tcggctgcaa aagtttgtgt agagataagc gtggcaaacg ctgtcgcaaa aagtacgcta
    10741 tgtcgcattg ttattccttt ttttggcagg gtgataatgc ccggcgttac cgggcaagtg
    10801 cagagttact taatgaatgg gcgggtcagc agaccgacag ggccagtggt gtaccagcga
    10861 cggttgccgc gactgcgtga gtcgcgccca acggcctgcg tcaggcggtc gaggataatg
    10921 gcgaggatca caatcccgac gccgccaacg gtggcaaggc ccatatccag acgaccgata
    10981 ccgcgaagta ccatctgacc cagcccgccg acggcaatca tcgaggcgat gaccaccata
    11041 gaaagagcca gcatcagcgt ctggttaacg cccgccataa tggtcggcat cgccagcggt
    11101 aactgaactt tgaacagcat ctggcgcggg ctggcaccga atgagcgcga ggcttcaatc
    11161 agatccgccg gaacctggtt aatccccaga atcgtcagac ggataatcgg cggcagagca
    11221 aagatgatcg tcaccactac gcccggcacg ttaccgatac caaacagcat gacgattggt
    11281 accagataaa caaacgctgg cgtggtctgc atggcatcaa gcagtggacg aataattttc
    11341 gccgctcgcg gacttctcgc cagccatatc cccaacggca aaccgatgac gatacagaac
    11401 agcagggcgg ttaacaccag cgccagagtc accattgcct gcgaccaggc accgattgcg
    11461 ccgatggcaa tcagcgaaac cagcgtcgcc acgcccattc cgaccccgga aatctgccag
    11521 gcgatgagag cgaaaacgat aatcgccact ggcgctggca tacccagcag caattgctgg
    11581 aaactgttga ggatataatc aaccggaacg cgcacgccct ggaagacggg acggaaatgg
    11641 gtaacaaccc agtcgatccc ttcagtgacc caactgtcga gcgggatcag cgttttatgg
    11701 aacggatcga gaatattaaa atgctcgacg tttggcgcag gcgtactggt cagccagtca
    11761 gcaccaccgc cgtcagtcgg tgcagtcgtc ggtgtacccc aggcgtctgc ggattgcgcg
    11821 gcactgtccg ccgctggcgt ggtatcccac ggattatttt gatcagccat tatttacccc
    11881 ctcacgatct aaagcgcgca gcagcattcc tttcgaaatg atgccgacat actgttggtc
    11941 ctcgtcgacc acgggcaccg cacagggtgc ctgtccgaca tgagagagca actcgctaag
    12001 aggcgtttgt gcatcgactg ctaacggcgc atcaatcagc gccgcatcaa gaccttgctg
    12061 ctgcgttaac gcggttttaa gcgaatcgat ggagactgcg ccgacaaact tattaccgcg
    12121 ttcgataacg tagccatatt cgcgatcttc atcctgcaat aatttcagtg ccgaacgtgg
    12181 gccgaagcca ggggttttac gaattaagcc attcggtgtc cggcgggcaa tatctttcgc
    12241 actgaatacc tgactaatat caacgccacg gaagaaggta cggacataat cattcgccgg
    12301 attattgaga atttcatccg gtgtgccgac ctgtaccact tcaccatttt gcataatggc
    12361 aattcggtcg ccaatacgca tggcttcatc gagatcgtgg gaaataaaga caatggtgcg
    12421 ctgatgtttc gcctgtaatt ttaccagctc atcctgcatc tcggtgcgaa ttaatggatc
    12481 gagcgccgag aaggcttcgt ccattaataa aatatccgga ttaatcgcta acgcgcgggc
    12541 taatcccaca cgttgacgca tcccgccaga gagttcatcc gggtagctgt gggcataatt
    12601 ttccagcccg acctgacgca gtgcatcaag ggctttttcc cggcgttctt cggcattaat
    12661 tccggccaat tccataccga acgcagtatt gtccagcacg gtcatatgcg gcattaaggc
    12721 aaaggactgg aagaccatcg caatcttttt tctgcgcacc tcacggagtt tggcatcaga
    12781 tattttggca atatccacac catcaatcag cacttgcccg cgggtgggtt caatcaggcg
    12841 attgagaagg cgtaccattg tggatttacc cgagccggat aatcccatga tgacaaatat
    12901 ctcgccttct tcaatggcca gactggcgtc ttttacgcca agcgatagcc cagttttttc
    12961 cagaatttgt tcttttgaaa gtccttgttc gatatatttg aatgctcgct gtggatgctc
    13021 accaaatatt ttataaaggt ttttaatttc taatttaatt gccatgcaat agaaagattc
    13081 ctgttatttg tttatgtcga tatattaccc aatggaaata gttacttttt tctaccctaa
    13141 catactgaga atctgaggca acccctgatg gcaaatgtgg catgaatatt gatgcgagca
    13201 aatcgccgga atttcccgtg atataagggc tgagagcaaa tcgagaaaag ttgatatttt
    13261 ttgtcaaaga tagcgaaaaa acgcctgaat attgtggttt caggcgaata atgcaacaga
    13321 gtatcagtgt agatcaccac aaaatatttg cgtgataaaa agctatttgg cgggaattat
    13381 ttccctgtcg cgggtagtga tatttttgaa aataacaccc tcagaaattc cagtcttcat
    13441 cttctgtttc aaccgctttc cccatcacat aagaggagcc tgaaccggaa aaaaaatcgt
    13501 gattttcatc ggcattcggc gaaagcgcgg cgaggattgc cggattcact tccgccattt
    13561 ctgcgggaaa taaaggttcg tagcccagat tcatcaaagc cttattggcg ttgtaacaga
    13621 gaaacgcttt cacatcgtca gcccacgggg tttcggcgta cagctcatcg gtgtattgca
    13681 gctcgttgtc gtagagttcc agcaacaaat cgaaggcgaa actcttcaac tcttcacgtt
    13741 gtcccagaga tatcttttcc atgtttttct gatatttata gcctatgtag taaccgtgga
    13801 ctgcttcatc acggataatc agacggatca ggtctgcggt attggtcagc tttccgcggc
    13861 tggaaaaata catcggcagc cagaaaccgg aatagaacaa aaaagattca agaaacacac
    13921 tggcgatttt ctttttcagc ggatcatcac cgcgataatg ttgctgaata atctgagctt
    13981 ttcgctgcaa cggtgcgttt tcttcactcc aggcgtaggc ggcatcgaca tctttggtct
    14041 ggcacagcgt cgagaaaatc gaactgtaag agcgggcatg aaccgcttcc ataaagctga
    14101 tattcgataa taccgcttct tcatgaggcg tgagtgcatc gggcatcagc gaaggcgcgc
    14161 cgataacatt ttgcagcgtg tcgagcagcg tcaggccagt aaaaacgcgc atcgtcagtt
    14221 gttgttctac gacagttaat gtctgccagg caggaatatc gttcgacagc ggcacctttt
    14281 ctggtagcca gaaattgctg gtcaggcgat tccacacctc cagatcttta tcgtcagata
    14341 tcttgttcca gttgatggcg ctgatacgtg agagtttcat agatattcct taaagtgcac
    14401 aggagacgca gccttcaatt tcagtgcctt ccagcgccat ctgacgcagg cgaatgtaat
    14461 agagcgtttt gatacctttg cgccaggcgt aaatctgcgc tttgttgata tcgcgagtgg
    14521 tggcggtatc ggggaaaaac agcgtcagcg acaaaccctg atcgacatgg cgagtcgctt
    14581 ccgcgtaggt gtcgatgatc ttttctgcgc cgatttcgta agcatcctga tacagcgcca
    14641 gattttcgtt agtcataaac ggggcagggt agtaaacgcg tcctgttttg ccctctttgc
    14701 gtatctctac tttcgccaca atcggatgaa tactcgacgt agcatggttg atataagaga
    14761 tagaaccggt tggcggcacc gcctgaagat tctggttgta aatgccgtag cgcatcacgt
    14821 cgtcgcgcag ttgcgcccac atctcacggg taggtaacgt aataccgctg cgggcaaaca
    14881 gttcgccaac tttcgccgtt ttcggctgcc agttaccttg cagatattgg ctaaaatatt
    14941 caccactggc ataacgtgac tgtttgaacc cggcgaaggt ttcaccgcgt tcgcgcgcca
    15001 gcaacatcga ggtacgcagt gcgtgccagg tgatggcata gaaatagaga ttggtgaaat
    15061 ccagtgcttc cggcgaacca taagcgatgc cttctcgcgc cagatagccg tgtaaattca
    15121 tctgccccag tccgatggcg tgtgaggcgg catttccggc ttcgatggac ggcacgctgc
    15181 ggatatgact catatctgac accgccgtta aaccgcgcac ggcagtctct accgtgcggg
    15241 caaaatcggg ggaatccatg gtgtgcgcaa tattcaacga acctaaattg caggagatat
    15301 catggcctgt gcgggcatag tcgagattct cgtcatactc tgaggcacag ttgacctgca
    15361 aaatctctga gcagagatta ctcatattta tgcgcccggc gacagggtta gcacggttta
    15421 ccgtgtcttc atacatgatg tagggatagc cggactcaaa ctggatttct gccagccgct
    15481 ggaagaaatc acgggcgttg aggtattttt tgcggatgcg ttcatcggca accagttcgt
    15541 catagtgttc gctgatggcg atatcggcaa acggcttgcc ataaactcgc tcaacgtcat
    15601 agggcgaaaa cagcgccatc tgcgcattct cttttgccag atggaaagtg atatccggga
    15661 tcaccacgcc cagcgacagt gttttaatgc ggattttttc gtcggcattt tcccgttttg
    15721 tatcgagaaa acgcagaata tcgggatgat gagcatgtaa atagactgca cctgccccct
    15781 gacgcgcgcc gagttggttg gcataggaaa atgcgtcttc cagcattttc atcaccggaa
    15841 ttacgccaga agattgattt tcaatacgtt taattggcgc gcccgcttct cgcagattcg
    15901 acagcaaaaa tgccacgccg ccgccgcgtt tcgacagttg cagtgcggaa tttaccgccc
    15961 ggccaatcga ctccatattg tcttcaatac gcagcagaaa acaggaaacc agttcgccgc
    16021 gctgctgctt accgcagttg aggaatgtcg gcgtggctgg ctgaaagcgt cctgacagca
    16081 tttcatcggt cagttgcaac gccagcgtcg catcgccttg tgccagcgtc agcgccacca
    16141 tcgtgactcg atcggcaaaa tcttccagat aacgtttacc gtcgaatgtc ttcaacgtat
    16201 agctggtgta aaacttccat gccccgagga atgtctggaa acgaaaaccg ctggtgtgtg
    16261 cgtgggcaaa cagcgtaatg acaaagtcgc gagagtagcg attaagaacg ctttcatcat
    16321 agtaaccttc gttgaccagc cagttcaggc gctgctgctg gctactgaag gtcacactgt
    16381 tcggacgcac atgcgtcgcc ataaaggcgt caacggcctg gcggtcttta tcgaactgaa
    16441 tgcgacctgc gctatcgtag aggttaagca tcgcattcag cgcgtggtaa tccatcgttt
    16501 cctgcgtcag gcattctgcg gttgtcgttg ccaaaattcg gttactcctt tacgaacgtt
    16561 ttcgatatcg ctttgcgtac ccatgagttc aaaacggtac agccacggca cgccgcattt
    16621 ccgggcaatc acatctccgg cgcggccata cgcctcacca aagttgcgat taccagaagc
    16681 aataacgccg cgaagcaacg cccggttgtg ctcgtcgttt aaaaagcgaa ttacctatcg
    16741 tggcaccgcg ccagccgtac cgccgccgcc gtaagagggc acgatcagga tgtaaggctc
    16801 gtctacctga atccgttccc gctcattgag cgggatgcgc accgcgggca gacctaaacg
    16861 ttcgataaaa cgctgcgtgt tttcggagct gctggagaag tagacgagct ggctcatgca
    16921 ctggccgcgt gtggcgctgg atgcagacgg ttaatcatgt ccggacggaa accagaccag
    16981 ctaagatcgc cagcaatcac taccggcaac tgacgaaagc cctgagcacg caacgcttcc
    17041 gctgcttcag gaacgcgatc gacattaatc atttcaaaat caaagccccg gttttccatc
    17101 gcccgtttgg tggcgtggca ctgaacgcaa tcgttacgag tgtaaatagt tatgcgcatg
    17161 attcgtattt ccgtttaaaa tgaagatacg gcgcgatgat acgcgtcggg ttgtctctct
    17221 gttgatacag agatactaga tgtagttgaa aaaagattca accacacaat atatagcaaa
    17281 tagtgatcga aattacccta gatatgagcg tgacggggga gggggatttt tgtgattcac
    17341 caggcaaaaa gaaaccccga agacaggctt cggggttaaa gacgcgtatt tattatcatt
    17401 tttgcactac gatttgcgca tgcttaacag tgcgccgata aaaataccta ccgcagctgc
    17461 tgtacccacg ctacaccagg gtctttcacg aacaaaagaa tccgcgcagc caacggcatc
    17521 gcgtgcggct tgctggacgc gagtacgacc atgcattcgc gccctggttt ctttcagcaa
    17581 tgcctgagct ttactgcgtg cggcttcagc ttcccctttg gcgtcgctgc cccaggactt
    17641 caatacagat tcgaggctgt cagctaattg attgacatca ttctggatat cctgcacgcc
    17701 atcatcaacg tcgttgcggt tcggtcggtt aaacatatga tcccccatta gaattcgatg
    17761 taattttcag tgtagaccat aattttctta actgaggttc atcgcggctt tatggacttt
    17821 tctgaaagcg ttgtgaatgc gcaatcaggt aaggtaggcc cgcggttgat aataacgagg
    17881 atcaaatatg tatttacgac cagacgaggt ggcgcgcgta cttgaaaaag tcggttttac
    17941 tgtcgatgtg gtaacccaga aagcttatgg ttaccgccgt ggggaaaatt atgtctatgt
    18001 taatcgcgaa gcgcgaatgg gacgtaccgc actggtaatt catccaacat taaaagaacg
    18061 tagttcgaca ctggctgaac ccgcttccga tattaaaact tgcgatcatt atcaacaatt
    18121 tccgctctat ttagcaggcg agcgacacga gcattacggt atcccgcatg gcttcagttc
    18181 gcgtgttgcg cttgaacgtt atttgaatgg tttatttggc gaagccagtt aaagacgcga
    18241 ctggcgatgc cagtcgcgaa aagaagagtc aggcttttac ctgctggtaa tggctgactt
    18301 taaacagtcg gcggcaataa tcgaggaagt agccataaac cgcccccatc aacatcgaaa
    18361 caacgatgtt tgaactgacc gccgccataa tctgatgcca gtctgcgccc accactaaca
    18421 agatcgccac atacaccggt gactggaacg tcacataagc caggatatca gccagatttt
    18481 ttatccagcc cgacgggcta actttgcgtg ccgcgcgcat aaatagatcg cggtacatac
    18541 cgtatggcca tgcaattaag atgttcaccg gaatcgctac caatctggaa taaaaagact
    18601 gttcgaagct cattccggag aggaaaactt caatacacat gttcacgaca gaacagtaaa
    18661 caaccatcgc gaacgtatct gcaactgcat gacgcaagcg tgactgcggt gagaacatgc
    18721 taatgctcct taataaaagc gtaatgggat gttaatggag atgaaagcag tggatctcat
    18781 tgccatctgc attttcgcat agcgtatatc gctggctatg actattcaat tagtgaaaaa
    18841 tttttattta gtcagttttt gtagataaaa tattccgtaa cggctcgttt tttgtacatt
    18901 gtggattttt acatgctgat atgatttatt ttcaattaaa tcaattagat atgaccttgc
    18961 ctttaatttc actaatgaaa gtttgggggt gaggtaacgc tataagcgta gagaattcgg
    19021 tgtatgtcgt gaccgtgtca tttacagcat ttttgatgcc agaaaatgta cattaacagc
    19081 agaaagcacc agtgaggggg g
//
LOCUS       DAJTZL010000075        18840 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.075, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000075 DAJTZL010000000
VERSION     DAJTZL010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18840
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.075"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(250..2121)
                     /gene="ppiD"
                     /locus_tag="OO544_002249"
     CDS             complement(250..2121)
                     /gene="ppiD"
                     /locus_tag="OO544_002249"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR6767834.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(2313..2585)
                     /gene="hupB"
                     /locus_tag="OO544_002250"
     CDS             complement(2313..2585)
                     /gene="hupB"
                     /locus_tag="OO544_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-beta"
                     /protein_id="HCR6767835.1"
                     /translation="MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVAL
                     VGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(2794..5148)
                     /gene="lon"
                     /locus_tag="OO544_002251"
     CDS             complement(2794..5148)
                     /gene="lon"
                     /locus_tag="OO544_002251"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HCR6767836.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(5336..6610)
                     /gene="clpX"
                     /locus_tag="OO544_002252"
     CDS             complement(5336..6610)
                     /gene="clpX"
                     /locus_tag="OO544_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HCR6767837.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(6736..7359)
                     /gene="clpP"
                     /locus_tag="OO544_002253"
     CDS             complement(6736..7359)
                     /gene="clpP"
                     /locus_tag="OO544_002253"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HCR6767838.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(7605..8903)
                     /gene="tig"
                     /locus_tag="OO544_002254"
     CDS             complement(7605..8903)
                     /gene="tig"
                     /locus_tag="OO544_002254"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HCR6767839.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(9247..9564)
                     /gene="bolA"
                     /locus_tag="OO544_002255"
     CDS             complement(9247..9564)
                     /gene="bolA"
                     /locus_tag="OO544_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HCR6767840.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            9869..10447
                     /locus_tag="OO544_002256"
     CDS             9869..10447
                     /locus_tag="OO544_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR6767841.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            10491..11966
                     /gene="ampG"
                     /locus_tag="OO544_002257"
     CDS             10491..11966
                     /gene="ampG"
                     /locus_tag="OO544_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HCR6767842.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPTGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            12427..13374
                     /gene="cyoA"
                     /locus_tag="OO544_002258"
     CDS             12427..13374
                     /gene="cyoA"
                     /locus_tag="OO544_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HCR6767843.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
     gene            13396..15387
                     /gene="cyoB"
                     /locus_tag="OO544_002259"
     CDS             13396..15387
                     /gene="cyoB"
                     /locus_tag="OO544_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HCR6767844.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            15377..15991
                     /locus_tag="OO544_002260"
     CDS             15377..15991
                     /locus_tag="OO544_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HCR6767845.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            15991..16320
                     /locus_tag="OO544_002261"
     CDS             15991..16320
                     /locus_tag="OO544_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HCR6767846.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            16332..17222
                     /gene="cyoE"
                     /locus_tag="OO544_002262"
     CDS             16332..17222
                     /gene="cyoE"
                     /locus_tag="OO544_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HCR6767847.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGFAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            17407..17718
                     /locus_tag="OO544_002263"
     CDS             17407..17718
                     /locus_tag="OO544_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HCR6767848.1"
                     /translation="MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            17702..18244
                     /locus_tag="OO544_002264"
     CDS             17702..18244
                     /locus_tag="OO544_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR6767849.1"
                     /translation="MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRAS
                     LKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEKQRVSGVLQGSQPSEIGVVRLVM
                     LGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
                     TPNAFGAVPMFLAIQHILAA"
     gene            complement(18300..>18840)
                     /locus_tag="OO544_002265"
     CDS             complement(18300..>18840)
                     /locus_tag="OO544_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HCR6767850.1"
                     /translation="GIMYANGQYVEVDCKKAKEYLDKGVHIYGGPEDFLATCRKDMID
                     RKTVDDTLPVITVTRSGMRDNFLDKGFSCMDSLFATTNKLGEVANLRVTFSIRRPSGK
                     EINQTVGFAPFGLNRLNISFTDYLFGSFTSNSSLILYKPEFERKSCATVRTTIVAATA
                     TINGKDVELLKAGAIEQKW"
BASE COUNT         4216 a         4952 c         4579 g         5093 t
ORIGIN      
        1 cgtcggtttc gccacagaag ctgccgccag cgcgctatga gacattccgg cacaggccag
       61 ggacagggta atgagcagtg ctttaattcc gtgtttcata ctgttttctc cttgtttgtt
      121 aacagtggaa tcaccttaac gggagtgaaa acagtaaaca aatggcaatg ttcagaaatg
      181 gaaaaggccg cgaaagcgac cttttcttgt tacagctgca acgcattttt ttgcgaggcg
      241 gcttcaggat tattgctgtt ccagcgcatc gccaattttg attttcgcct ctttacgcag
      301 gttactcatc agagcttcaa agacgatttg tgcgttgttc tgggtgatac cctgcaccat
      361 cgcttttttc tgatcttccg gcattgaacc ttgtttcact tcatccagcg ccagcagaac
      421 cacattacct tgcatatcgg tcgccatacc gtagctcggt ttgtctttcg ctggcagtgg
      481 cagtgcaaac gccgcctggc taatcgggtc acgaccggaa cggcttaagg ttttcggctc
      541 gccaaatttc agaccggcag cctgcatagc ttccgcccct ttgccggctt tcaaatcaac
      601 cagcagtttc tcagcatcca ctttcgcctg ttgttcagct ttgttgtgct gaaccagtgc
      661 cttaacttgt tcctgaacat ctgccaacgg tttcaccgct tccggtttgt gctcgctgat
      721 gcgcagcacg aatgcgcggt cgccgtctac ggtgatgatg tcagagttga tgcccggcgc
      781 gccgttttca cctaccagac cgccgttaaa gatagcgtcg gcaaccggct tgaagttcaa
      841 ctcttccggc aggttatctt tgctgaacca acccgtctga gtggctttaa cgccggcagc
      901 ttgctctgca ccggccagag actcggtgtc gttgcttgcc gcatcgctca ctttctgctg
      961 cagcgcatag tacgcatcga gggctttttc gtgtttcact ttcgccgcaa tgtcgtcacg
     1021 tacttcgtct aacgatttca ctttcgctgg ctgaatgtcg tccagacgta caatcaggaa
     1081 accgaccgaa gatttgatga caccagacag ttggcctttt tctttcagac cagcattttt
     1141 cagttcgtcg gggatagtgg catcttctaa ccaacccata tcgccgccgt tacgggcaga
     1201 gataatatcg gcagattttt ctttggctaa tgcagcaaaa tcaccgcctt tattcagctc
     1261 atcaagtacc gctttcgctt catcttcagt tttggtctgg atgatgctgt agcgggtacg
     1321 ctgcggctgg gtgaattgat cctgatgctg atcgtagtag ctctggatat ccgcatcgct
     1381 aaccggttgc tgcatcgttg cggcatccag cttgatgtaa ctcacgcgga attgttccgg
     1441 cgtcatgaaa ttgtttttgt tttgttcgta gtagctggca atctcctgtt cggtcacagg
     1501 ctgcttcgcc gccagcgcgt taacatcgat agtcgcctca cgcaccacgc gttgttgcgc
     1561 gaccagtgcc gccagctcgt cggtttcacc tttcagcata aaatcggtac cggcaacacc
     1621 gttaatcagc tgttgggtag tgagctggtt acgcagcgcc tgggcgtact gatcggcggt
     1681 catccccatc tggttgagga taccgttata gcggctgtta tcaaatttgc cgtcaacctg
     1741 gaaggctggg gtcgcgaaaa tcgcctgttt aacctgctca tcactgatac ccagtttcag
     1801 ctcacgagcg tactgatcca acagcgcctc gtcgatcaga cgattcagca cctgttgacg
     1861 cagggttttc atatagcctt cgttcgctgc cagctcggag tattgatcgc ccagctgttg
     1921 ctgcatgcga ttacgctcgc tgttaaaggc gttctcgaac tgcccacggc tgatttcctg
     1981 gtcattcact tttgcggcgt agttattgcc tccgccaatc aggtaaccac tcacgccggt
     2041 caatatgaac gacacgataa tgataccgaa aataatcttg agcacgagac tgtttgcagc
     2101 cgtgcgtaag ctgtccatca tggtgtaaca acactccgct gtagatgact gtgtacctca
     2161 cgcaacgtat cgatgcgcgc aaaggcctat tgtgacaaga aacgggggca attgtcagcc
     2221 cacaagacac agaaagtcac cgaatacaaa taaaaaaggc acatcagtag atgcgccctt
     2281 gaacttcgtc acatccccac tggggacaac gcttagttta ccgcgtcttt cagtgcttta
     2341 cctgcacgga agctcggtac tttagcggca gcgatggtga tctctttacc ggtctgcggg
     2401 ttgcggccag tacgggcagc acgctcttta acggcaaaag taccaaaacc taccagtgct
     2461 acatcatccc cttctttcag agattcagtt acggaagcaa taatagcatc taacgcacgg
     2521 ccagccgcag ctttagagat atcagcccct gcagcaatct tgtcgatcaa ttgagattta
     2581 ttcactcttc tcttcctctt tataatttat atcgcacttg aatccttcaa ggtacgaacg
     2641 cgcagcagtt atatcaggcc agccatcccc ttacaacacc ccttaataag ggcaagcccg
     2701 atccgccatc taacttagcg agacaaaaaa aggctggcaa gcccgaatta gcctgccagc
     2761 cctgttttta ttagtgcatt ttgcgcgagg tcactatttt gcagtcacaa cctgcatgcc
     2821 agacggttca ttttgcagcg ccagagtcag aacttcctca atgcgcttca caggatgaat
     2881 gtccagatcg gcaattacgt tgtcaggaat ctcttccaga tcgcgtttat tttcgaacgg
     2941 aattagcact gttttaatcc cgccgcgatg cgctgccagg agtttttctt tcaaaccacc
     3001 gatcggcagt acctgaccac gcagagtgat ctcaccggtc attgccacat cggcacgaac
     3061 cgggttaccg gtcaggcaag aaaccagcgc ggtgcacata gcaataccgg cactcggacc
     3121 atctttcggc gtcgcacctt ccggtacgtg gacgtggatg tcacgttttt cgtaaaaatc
     3181 agggttgatc cccagttttt ccgcacgcgc acgaaccacc gttaacgccg cctgaataga
     3241 ctcctgcatc acttcgccga gcgaaccggt ataggtcagt ttgcctttac ccggaacaca
     3301 tgcggtttca atggtcagca agtcaccgcc cacttccgtc cacgccagac cggttacctg
     3361 accgacacgg ttttcgttat cagcgcgacc atagtcgaaa cgctgaacac cgaggtagtc
     3421 atgcaggtta tcgccgttaa tttcgatatg ttttaatgac ttatcgagca gtaactgctt
     3481 aaccgctttg cggcacagtt tggagatttc acgctccaga ccacgcacgc ccgcctcacg
     3541 ggtgtagtaa cgaataatgc cgataatggc gctatcgtcg acggtcagct cacctttttt
     3601 cagtgcatta cgttcaatct gcttcggcag caggtgacgt ttggcgatgt tcagtttttc
     3661 atcttcggta taaccggaga ggcgaatcac ttccatacga tccagcagcg gtgccggaat
     3721 gttcatggag ttcgacgtcg cgacaaacat cacgtcgctg agatcgtaat ccacttccag
     3781 gtagtggtcg ctgaacgcta cgttctgctc tggatccagc acttcaagca gtgcagaggc
     3841 cggatcgcct cgcatgtcag aagacatttt gtcgatctca tcgagcagga acagcgggtt
     3901 tttcacgccc actttcgcca ttttctggat cagtttaccc ggcatagaac cgatgtaagt
     3961 acggcggtga ccacggattt ccgcttcatc acgcacgccg cccagcgcca tacggacata
     4021 tttacgcccg gtggctttgg caatggactg accaagagag gttttaccta cccccggcgg
     4081 ccctaccagg cagaggatcg gtcccttgat tttgttgaca cggctttgaa ccgcaaggta
     4141 ctcaaggatg cgatctttca cgcgctccag accataatgg tcggtatcaa ggatttcctg
     4201 cgcctgacgc aggtcttttt tgaccttgct gcgcgcattc cacggtacct gtaccatcca
     4261 gtcgatataa ccacgcacta cggtcgcttc tgccgacatc ggagacatca ttttcagctt
     4321 ctgcaactct gcttccgctt tctcttttgc ctctttcggc atcttcgccg cgtcgatttt
     4381 gcgcttcagg gcttcgtttt cgtccggcgc gtcgtccatt tcaccgagtt ctttctgaat
     4441 agctttcatt tgctcgttca gatagtactc acgctgggat ttctccatct gctttttaac
     4501 gcggttgcga atgcgtttct caacctgcag cagatcgatt tccgattcca tcattgccat
     4561 cagatattcc agacgttcgt taacgtcgga catctccaga acggactgtt tgtcagccag
     4621 tttcagcggc atatgtgcag caatggtatc cgccagacgc gccggatcgt cgatgctatt
     4681 cagcgacgtc agcacttctg gtgggatttt tttgttcagc ttgatgtagc cttcgaactg
     4741 gctgattgca gtacgcacca gcacttcctg ttcccgctca tcaatggtcg gcgactccag
     4801 atactccgcc ttcgcagaaa agtgttcgcc attgtcagag agcgcagaaa tacgcgcgcg
     4861 ctgtaacccc tcgaccagca ctttgacggt gccgtcaggc agtttcagca tctgcaatat
     4921 agaggccacg gtcccgacgg tgaaaagatc gtttacaccc ggctcatccg ttgaagcttc
     4981 tttctgcgcg accagcataa tttttttatc atggtccatc gccgcttcca gacaacggat
     5041 agatttttcc cgcccgacaa ataaggggat gaccatgtgc ggataaacca ccacatcgcg
     5101 cagcggcaat acggggattt caatgcgttc agaacgctca ggattcatag agctctctct
     5161 tagtttaatt tccgccaggt aatcagatga cacgactgtg cttcacgcca tctattaaca
     5221 tgtacgtcag tatatgggga tgtttccccc acattcaacg ccgagaatag aggaaaaatt
     5281 aaaggggaga taaaatcccc cctttttggt taactaattg tatgggaatg gttaattatt
     5341 caccagatgc ctgttgcgct tccggcttac cataaatcag caacggtttg ctttgaccat
     5401 caattaccga ctcgtcgata accacttttt cgacgtcttc catggacggc agatcgtaca
     5461 tggtatcgag cagtgcggct tctacgatgg aacgcaggcc acgggcaccg gttttacgcg
     5521 ccatcgcttt cttagcgata gcatccagcg cctcgtcacg gaattccaga tccacgcctt
     5581 ccagattaaa cagcgcctga tactgcttgg tcagggcgtt tttcggctct ttgaggatct
     5641 gaatcagagc ttcttcgctc agttcattca acgttgctac aaccggcaga cgaccaatga
     5701 actcagggat aagaccaaac ttgatcagat cttccggttc aacctgcgcc agcagctcgc
     5761 cttcgcttgc tttgtcggac ttcgctttta ccgtcgcgcc aaaaccaatg ccggagccgg
     5821 tttctacacg gtgggaaatc actttatcca gaccggcaaa cgcaccgcca caaataaaca
     5881 ggatcttaga ggtatcaacc tgcaagaatt cctgctgcgg atgtttacgc ccaccttgcg
     5941 gtggaacagc agctaccgta ccttcgatca gtttcaacag tgcctgctgt acgccttcac
     6001 cggaaacgtc tcgggtaatg gacgggttgt ctgacttacg agaaatcttg tcgatttcat
     6061 cgatgtagac aatcccgcgc tgcgctttct gtacgtcgta atcgcacttt tgcaacagct
     6121 tctgaatgat gttttcaacg tcttcgccca cataaccggc ttcggtcagc gtggttgcgt
     6181 cggccatggt gaacgggacg tccagcaggc gcgccagcgt ttcagccagc agcgttttac
     6241 cggaaccggt cggaccgatc agcagaatgt tacttttgcc caactcgaca ccattgctgg
     6301 tatcgccgtt gcgcagacgt ttgtaatggt tgtataccgc gaccgccagc acttttttcg
     6361 cctgttcctg gccgataacg taatcgtcca ggtggttgcg aatttcatgc ggcgtcggta
     6421 gcgcactgcg ttcacgatgc ggtgcaactt ctttaatctc ttcgcgaatg atgtcgttac
     6481 ataaatcaac acattcgtcg cagatataca cggatggacc ggcaatcagc ttgcgcactt
     6541 catgctggct tttgccgcaa aaagagcaat acagcaattt gcctgagcca tctttgcgtt
     6601 tatctgtcat gggtcaaaac ctcttctttg ttctttgtgc cgcacacgac gacgcaaatg
     6661 ccattctcag gcgcaagccg cttacagcgt tgtgccgccc tggataagta tagcggcaca
     6721 gttgcgcctc tggcatcaat tacgatgggt cagaatcgaa tcgaccagac cgtattccac
     6781 cgcttcaggg gcggaaagga agcgatcgcg ctcggtatca cgttcaatct gttctaatga
     6841 ttgacccgta tgaagcgcca taagttcatt catgcgccct ttaactttca gaatttcacg
     6901 ggcatgaatt tcgatatcgg tcgcctggcc ctggtagccg cccaacggtt ggtgaatcat
     6961 cacgcgcgaa ttcggcaggc aaaaacgttt accttttgcc cctgcggtca gcaagaaagc
     7021 gcccatcgag gccgcctggc ccatacagat ggtgctgaca tcaggcttga taaactgcat
     7081 ggtgtcatag atagacatcc cggcagtgat caccccgcct ggggagttaa tgtacagata
     7141 gatatctttt tctgggtttt ccgcttctag gaacagcatc tgcgccacaa tcaggttcgc
     7201 catgtggtct tcaacctggc cagtcagaaa aatgacgcgt tccttaagta gacgagaata
     7261 gatatcaaaa gagcgctcac cgcgtgaggt ctgttcaatg accatcggca ccagcgccat
     7321 atggggtgca aagttatctc gttcgccgct gtatgacatt tccgtctcct ggataaaatt
     7381 gaaaaaacct gctgtaccga ttgtaacctt atggacggat tatcagctag tccctgtgtt
     7441 atgggtacgg catcaccata tttcaagcat aacaatcttt tgttattacg ctaacaccga
     7501 aagaggcttt tcgcacggtt ccatgcaaaa ttcatgacaa aaaaaagccc accacctggc
     7561 ggtgacgggc ctttgtgcga atttagcgcg ttatgctgcg taaattacgc ctgctggttc
     7621 atcagctcgt tgaaagtggt ttctttttca gtcactttcg ctttcgccag tacagcttca
     7681 acagcctgtt cttccagagc aacattgcgc atgttgtcca tcagttcttt gtttttgctg
     7741 tagaactcga taacttcttt cggatcttcg tacgcagaag ccatctcttc gatcaggcct
     7801 ttcacgcgct cttcgtcagc tttcagttcg ttggtgcgga taacttcgcc cagcagcagg
     7861 ccaacaacta cgcggcgttt agcctgttct tcgaacagtt cgcgcggcag ttccagagct
     7921 tgtttttcgt tgccaccgaa acgctgtgca gcctggcgac gcagaacgtc gatttcgctg
     7981 tcgatcagcg cagccggtac gtcgatgtcg ttagctttta ccagaccttc gatcgcctga
     8041 gacttaacgc ggttacggat ggcgctcttc agctcgcgct ccatgttttt acgcacttca
     8101 gcgcgcagac cttctacgga accatcttca acgccgaaac gtttgatgaa ttctgcagtc
     8161 agttccggca gttcacgctc ttcaactttc ttcaggttga tagcgaattt cgctgcttta
     8221 cctttcagat tttctgcgtg gtattcttcc gggaaggtca cgtcgatggt gaactcttcg
     8281 ccagctttgt ggcctttgat accgtcttca aagcccggga tcatacgacc ctggcccatc
     8341 gccagtacga aatcagacgc tttaccgcct tcgaactctt cgccgtctac agaaccggtg
     8401 aagtcgatgg ttacgcggtc ttctgcttca acggcgccgt ctttttcttt ccaggtcgcc
     8461 tgctgtttac gcagagtatc cagcatgccg tcaacgtcag cgtcggtcac ttcaacgatc
     8521 ggtttttcaa cttcgatcgc ttccagaccc tgcagttcaa cttccggata aacttcaaac
     8581 tctacagagt aggtgaagtc ttcacccagc ttgtattcgc ccggaacata agtcggtgcg
     8641 ccagccggat tgattttttc tttaatgatg gcgtcaatga agttacggct catcaggtca
     8701 cccagaacgt cctggcgtac agacgcgcca taacgctgag caacgatatt cattggcact
     8761 ttgcctttgc ggaagccgtc aatacgtact tttttcgcaa cgttgaccag ctcgctttta
     8821 acagcggtct cgatgctgtc agcagcgata gtaatcgtta cacggcggcc aaggccttga
     8881 gtggtttcaa ctgaaacttg catcttgtta cctcaaaaaa tcacagtgct cggtcaactc
     8941 ttaccccgca agcacaggtt gttggcttcg cggaaccggg atgttcttat aatcaatcac
     9001 attccctgtt accagaatca tcccgaaagc attcaaatag gacgcggcat tatagcggca
     9061 tcacttttat gagtcgagaa cggttattgc gcgctgtcgc gtattttttc acaattcccg
     9121 ggcaattttc gtctgaatgc tgggcaaaaa gcgcaaaatc cagacaaaac aaaacggccc
     9181 gcaggccgtt tttcataaaa tctttagcaa catactggaa aagcgccgac agttgcaaat
     9241 gcgtttttac gcgatgcttc ctgctccacg acagggagga gaggcaaaga cggtgtcctg
     9301 caacccttcc cactccttaa tagtgtaagt atgcagagcc agcgcatgaa cggtagtaga
     9361 gagttcctcc gctaaagtac tgtaaatcat tcgatgacga ttcagaaaac gttcacccgt
     9421 aaaacgatcg ctgaccagca caactttaaa atggctttca gagccggctg ggacattgtg
     9481 acgatagctt tcatccacta cttcgaggaa tacgggttgg aacgccgccc ttaatttttc
     9541 ttctatccgc tcacgtatca tcatgaaata tctcctccga caacgctgag atgtcatcca
     9601 tccctttaaa tactagccgc ttttaccgtt tccattgcag cttaacaaca aatatttagg
     9661 tttcgtcctg gtttttccgt caagcgtatg aagtttactg atttaaagat tgaaacacag
     9721 gcctcgcatc agcgtttctt ttaccagggg tgattatttc gccaacatga tgaatttaca
     9781 tgccttaccc acttcccctc gccgttggca atgttatgat ggcgggaatt tttaccaaac
     9841 acgcttaaga gtctctgaga gcccgaacat gttcaaaaaa atcctcttcc cgttagttgc
     9901 tctgtttatg cttgcaggat gcgcaaaacc gccaacaact attgaagttt ccccgacgat
     9961 tacactgcca cagcaggatc caagcctgat gggcgtcacc gtaagcatta atggtgccga
    10021 tcagcgtacc gatcaggcgc tggcaaaagt cacccgcgat aatcaaatcg ttaccctgac
    10081 cgcctcccga gatctgcgtt tcctgctgca agaagtgctg gaaaaacaga tgaccgcgcg
    10141 tggttacatg gttggtccga atggcccggt taatctgcaa atcatcgtta gccaactgta
    10201 tgctgacgtg tcccagggca acgtgcgcta caacatcgcg accaaagcgg atatcgccat
    10261 catcgctacc gcgcagaatg gcaacaaaat gaccaaaaac tatcgtgcca gctacaacgt
    10321 tgaaggtgcg ttccaggcct ccaacaaaaa tatcgccgat gcggttaaca gtgtgctgac
    10381 cgataccatc gctgatatgt ctcaggacac cagcatccac gaattcatca agcagaacgc
    10441 gcgttaattt ctgccctctg gcccggtgca aaccgggcct gtagacgccc atgtccagtc
    10501 aatatttacg tatttttcaa cagccgcgtt cagccatatt gctgatcctg ggttttgctt
    10561 ccgggctacc gctcgccctg acatccggta ccttacaggc ctggatgacg gtcgagaata
    10621 ttgatctcaa aaccattggt ttcttctctc tggtaggcca ggcttacgtt tttaaattcc
    10681 tctggtcacc gctgatggac cgctacacgc ccccattttt tgggcggcgg cgcggttggc
    10741 tgctcgccac gcaaatcctg ttattagtcg ccattgcggc gatgggtttt ctcgaacctg
    10801 gcacccaact ccgctggatg gcggcactgg cggtggtgat cgctttttgc tcggcctccc
    10861 aggatattgt cttcgatgcg tggaaaaccg atgtgctccc ggcagaagaa cgtggtgcgg
    10921 gcgcggcaat cagcgtgctg ggttaccgtt tagggatgct ggtttccggc ggcttagccc
    10981 tgtggctggc agataaatgg ctgggctggc agggcatgta ctggttgatg gcggcgctgt
    11041 tgatcccttg tattatcgcg acgttgcttg caccagaacc gaccgacacc attcctgtgc
    11101 caaaaacgct ggaacaagcg gttgttgcac ctctgcgaga tttctttggt cgcaataatg
    11161 cctggcttat tttgcttctt atcgtgctgt ataagctggg cgacgcattc gccatgagcc
    11221 tgacaaccac gtttttgatt cgcggcgtcg ggtttgatgc gggtgaagta ggcgtggtta
    11281 acaaaacgct tggcttactg gcgaccattg tgggcgcatt gtacggtggg attttgatgc
    11341 agcgcctgtc gctgttccgg gcgctgctga ttttcggcat tttacaaggt gcgtctaacg
    11401 ctggttactg gctgctgtcg attactgata agcatctcta cagcatgggc gcagccgtct
    11461 ttttcgaaaa cctctgtggc gggatgggca catcagcctt tgtcgcactg ttaatgacgc
    11521 tgtgtaataa gtcattttcc gctactcaat ttgccctgct ctcagcgctt tccgctgtag
    11581 gacgagttta tgtcggcccc gtggcgggtt ggtttgttga agcacacggc tggtcgacat
    11641 tctatctatt ctccgtcgcc gctgccgtac cggggcttat tttgctgctg gtttgccgcc
    11701 agacgcttga atatacacga gtaaatgaca actttatctc ccgtaccgaa tatccgacag
    11761 gttatgcctt tgccatgtgg acactggcgg cgggcgtcag cctgttggcc gtgtggttac
    11821 tgctgttgac gatggacgcg ctggatttga cgcacttctc tttcctgcct gctctgctgg
    11881 aagtcggggt tttagtcgcc ctttctggcg tcgtgcttgg tggtttgctg gattatctgg
    11941 ctctacgaaa aacgcatctg acgtaatctg taaatattat ttagctgtag taatcactcg
    12001 ccgtaattat tacggctaaa taaacatcag tatcgcttaa taatatttgt tcttttgtgc
    12061 ggcttagcgt ttggaaatta ttgacgcaat ttatgaattc tattttttct tacacgattc
    12121 agctaatgag tctttatttt ccccccaccc aattgtcacc ataatgataa ttatttgtta
    12181 aacaattgtt ttattcaaga ttggttatac caattgcccg tccaaactcc ttctgcactc
    12241 ccctttcgtt ataacgccct tttgcaacag cttcttaaaa tcaacttgat atgttttgca
    12301 acatatgtaa cctgacagcc aaatccaagt aacaggaatt taatcatgtt tacagtaatg
    12361 taacctcccc gtaaaatgcc cacacacttt aaacgccacc agatcccgtg gaattgaggt
    12421 cgttaaatga gactcaggaa atacaataaa agtttgggat ggttgtcatt atttgcaggc
    12481 actgtattgc tcagtggctg taattctgcg ctgttagatc ccaaaggaca gattggtctg
    12541 gagcaacgtt cactgatact gacggcattt ggcctgatgt tgattgtcgt tattcccgca
    12601 atcttgatgg ctgttggttt cgcctggaag tatcgtgcga gcaataaaga tgctaagtac
    12661 agcccgaact ggtcacactc caataaagtg gaagctgtgg tctggacggt acctatctta
    12721 atcatcatct tccttgcggt actgacctgg aaaaccactc acgctcttga gcctagcaag
    12781 ccgctggcac acgacgagaa gcccattacc atcgaagtgg tttccatgga ctggaaatgg
    12841 ttcttcatct acccggaaca gggcattgca accgtgaatg aaatcgcttt cccggcgaac
    12901 actccggtgt acttcaaagt gacctccaac tccgtgatga actccttctt cattccgcgt
    12961 ctgggtagcc agatttatgc catggccggt atgcagactc gcctgcatct gatcgccaac
    13021 gaacccggta cttatgacgg tatctccgcc agctacagcg ggccgggctt ctcaggcatg
    13081 aagttcaaag ctattgcaac accggatcgc gccgaattcg accaatgggt cgcaaaagcg
    13141 aaacagtcgc cgaacaccat gtctgacatg gctgcgttcg aaaaactggc cgcgcctagc
    13201 gaatacaacc aggtggaata tttctccaac gtgaaaccag acttgtttgc cgatgtgatt
    13261 aacaagttta tggctcacgg taagagcatg gacatgaccc agccagaagg tgagcacagt
    13321 gcacacgaag gtatggaagg catggacatg agccacgcgg aatccgccca ttaaaggggt
    13381 tgaggaagaa taaagatgtt cggaaaatta tcacttgatg cagtcccgtt ccatgaacct
    13441 atcgtcatgg ttacgatcgc tggcattatt ttgggaggtc tggcgctcgt tggcctgatc
    13501 acttacttcg gtaagtggac ctacctgtgg aaagagtggc tgacctccgt cgaccataaa
    13561 cgcctcggta tcatgtatat catcgtggcg attgtgatgt tgctgcgtgg ttttgctgac
    13621 gccattatga tgcgtagcca gcaggctctt gcctcggcgg gcgaagcggg cttcctgcca
    13681 cctcaccact acgatcagat cttcaccgcg cacggcgtga ttatgatctt cttcgtggcg
    13741 atgcctttcg ttatcggtct gatgaacctg gtggttccgc tgcagatcgg cgcgcgtgac
    13801 gttgcgttcc cgttcctcaa caacttaagc ttctggttta ctgttgttgg tgtgattctg
    13861 gttaacgttt ctctcggcgt gggcgaattt gcgcagaccg gctggctggc ctatccaccg
    13921 ctatcgggaa tagagtacag tccgggagtc ggtgtcgatt actggatatg gagtctccag
    13981 ctatccggta taggtacgac gcttaccggt atcaacttct tcgttaccat tctgaagatg
    14041 cgcgcaccgg gcatgaccat gttcaagatg ccagtattta cctgggcatc actgtgcgcg
    14101 aacgtactga ttattgcttc cttcccaatt ctgacggtta ccgtcgcgtt gttgaccctg
    14161 gatcgctatc tgggcaccca tttctttacc aacgatatgg gtggcaacat gatgatgtac
    14221 atcaacctga tttgggcctg gggccacccg gaagtttaca tcctgatcct gcctgttttc
    14281 ggtgtgttct ccgaaattgc ggcaaccttc tcgcgtaaac gtctgtttgg ttatacctcg
    14341 ctggtatggg caaccgtctg tatcaccgtg ctgtcgttca tcgtttggct gcaccacttc
    14401 tttacgatgg gtgcgggcgc gaacgtaaac gccttctttg gtatcaccac aatgattatc
    14461 gccatcccga ccggggtgaa gatcttcaac tggctgttca ccatgtatca gggccgcatc
    14521 gtgttccatt ctgcgatgct gtggaccatc ggttttatcg tcactttctc ggtgggcggg
    14581 atgactggcg tgctgctggc agtaccgggc gcggacttcg ttctgcataa cagcctgttc
    14641 ctgattgcgc acttccataa cgttatcatc ggcggcgtgg tcttcggctg cttcgcaggg
    14701 atgacctact ggtggcctaa agcgttcggt ttcaaactga atgaaacctg gggtaaacgc
    14761 gcgttctggt tctggatcat cggcttcttc gttgccttta tgccgctgta tgcgctgggc
    14821 ttcatgggca tgacccgtcg tttgagccag cagattgacc cgcagttcca caccatgctg
    14881 atgattgcag ctagcggtgc agtactgatt gcgctgggta ttctctgcct cgttattcag
    14941 atgtacgttt ctattcgcga ccgcgaccag aaccgtgacc tgactggcga cccgtggggt
    15001 ggccgtacgc tggagtgggc aacctcttcc ccgcctccgt tctataactt tgccgttgtg
    15061 ccgcacgttc acgagcgtga tgcattctgg gaaatgaaag agaaaggcga agcgtataaa
    15121 aagcctgacc actatgaaga aattcatatg ccgaaaaaca gcggtgcagg tatcgtcatt
    15181 gcagctttct ccaccatctt cggtttcgcc atgatctggc atatctggtg gctggcgatt
    15241 gttggcttcg caggcatgat catcacctgg atcgtgaaaa gcttcgacga ggacgtggat
    15301 tactacgtgc cggtggcaga aatcgaaaaa ctggaaaacc agcatttcga tgagattact
    15361 aaggcagggc tgaaaaatgg caactgatac tttgacgcac gcgactgccc acgcgcacga
    15421 acacgggcac cacgatgcag gcggaaccaa aatcttcgga ttttggatct acctgatgag
    15481 cgactgcatt ctgttctcaa tcttgtttgc tacctatgcc gttctggtga acggcaccgc
    15541 aggcggcccg acaggtaagg acattttcga actgccgttc gttctggttg aaactttctt
    15601 gctgttgttc agctccatca cctacggcat ggcggctatc gccatgtaca aaaacaacaa
    15661 aagccaggtt atctcctggc tggcgttgac ctggttgttt ggtgccggat ttatcgggat
    15721 ggaaatctac gaattccatc acctgattgt taacggcatg ggtccggatc gcagcggctt
    15781 cctgtcagcg ttctttgcgc tggtcggcac gcacggtctg cacgtcactt ccggtcttat
    15841 ctggatggcg gtgctgatgg tacaaatcgc tcgtcgcggc ctgaccagca ctaaccgtac
    15901 ccgcatcatg tgcctgagcc tgttctggca cttcctggat gtggtttgga tctgtgtgtt
    15961 cactgttgtt tatctgatgg gggcgatgta atgagtcatt ctaccgatca cagcggcgcg
    16021 tcccatggca gcgtaaaaac ctacatgaca ggctttatcc tgtcgatcat tctgacggtg
    16081 attccgttct ggatggtgat gacaggagct gcctctccgg ccgtaattct gggaacaatc
    16141 ctggcaatgg cagtggtaca ggttctggtg catctggtgt gcttcctgca catgaatacc
    16201 aaatcagatg aaggctggaa catgacggcg tttgtcttca ccgtgctaat catcgctatc
    16261 ctggttgtag gctccatctg gattatgtgg aacctcaact acaacatgat gatgcactaa
    16321 gagcggcggt tatgatgttt aagcaatacc tgcaagtaac gaaaccaggc atcatctttg
    16381 gcaacctgat ctcggtgatt gggggattcc tgctggcctc aaagggcagc attgattatc
    16441 ccctgtttat ctacacgctg gttggggtgt cactggttgt ggcgtcgggt tgtgtgttta
    16501 acaactacat cgacagggat atcgacagaa agatggaaag gacgaagaat cgggtgctgg
    16561 ttaaaggcct gatctctcct gctgtctcgc tggtgtacgc caccttgcta ggctttgctg
    16621 gctttatgct gctgtggttt ggcgcgaatc cgctggcctg ctggctgggg gtgatgggct
    16681 ttgtggttta tgtcggcgtt tatagcctgt acatgaaacg ccactcggtc tacggcacat
    16741 tgattggttc gctctccggc gctgcgccgc cggtgattgg ctactgtgca gtaaccggcg
    16801 agttcgatag cggcgcagcg atcctgctgg ctatcttcag cctgtggcag atgcctcact
    16861 cctatgccat cgccattttc cgctttaagg attatcaggc ggcaaacatt ccggtattgc
    16921 cagtggtaaa aggcatttcg gtggcgaaga atcacatcac gctgtatatc atcgcctttg
    16981 ccgttgccac gctgatgctc tctcttggcg gttacgctgg gtataaatat ctggtggtcg
    17041 ccgcggcggt gagcgtctgg tggttaggta tggccctgcg cggttataaa gttgctgatg
    17101 acaggatctg ggcgcgcaag ctgttcggct tctctatcat cgccatcaca gccctctcgg
    17161 tgatgatgtc cgttgatttt atggtgccgg actcgcatac gctgctggct gctgtttggt
    17221 aacacaacct ctctattaaa aaggtgctac ggcacctttt ttattgtctg agatcagaga
    17281 tacggctaat aaccgtatgt cttgacagag tgagcagatg aacataaatt aatcatacgg
    17341 ttgatagccg tattatgtat gagatcgact ccctacgatt tcattcagtt aatcaaagag
    17401 gtcataatgc ctggaaaaac agccactcta cccgatgtcg ataaaacgct gaaaaatgcg
    17461 cgggtcgaac tcaaaactag tcccgacgcc aaaaacaaac ttcgtgaagc tgcgcaagcc
    17521 gttggggtag atttaagcgc ttttattttg agcgctgcta tggaacgcgc cgaaagtgtg
    17581 cttgataacc aacgtcgccg tgagctttcg aatcaaagct gggaactgat gaaccaactc
    17641 atcgctgaac ctgctcaacc gacgctcgcc ctcaaggcgt taatgaaaag gaaaaacagc
    17701 gatggtcgac aagcatgaag agattactct gcccatagtc ctctcctgta attatcagtc
    17761 tgatattact tatcctgggc aaaaacagtt tgattgcggt aaccctgtta tcgataaatt
    17821 tgtacgcgca tcgctaaaga aaagtgtgcg taatagcgac tgtgcggcta aagcacttat
    17881 tgacagacaa agtggtgaac tgatcggcat ctgtactttt acggcatatt cgctggaaaa
    17941 acaacgcgtt tctggcgtcc ttcagggttc acaaccttca gaaattggtg ttgtcagatt
    18001 agtcatgttg ggggtagcac ggaagtatca aaagcggggc tttggtcagg acctactatg
    18061 tgattttttt gaacatgtaa aaataattca ccaggcatta ccaattaaag gggtttatct
    18121 tgatgctgac cctgccgcca ttaattttta tgctcgtctc ggctttgttc agctttcagc
    18181 gacaccaaat gcttttggtg ctgtacctat gtttttggcg attcagcata ttctcgcggc
    18241 ttagattatt atcgccaccc gccagggcaa gttttgcctt agccgggggc tgagcagcgt
    18301 taccactttt gttcaattgc ccccgctttc agcaactcca catccttgcc gttaatggtt
    18361 gccgttgcgg caacgattgt ggtccttaca gttgcacaag attttcgctc aaattccggt
    18421 ttataaagaa taagagacga attactggta aatgaaccaa ataggtaatc agtaaagcta
    18481 atattcagcc ggtttaaacc gaaaggcgcg aagccaaccg tttggttaat ctccttaccg
    18541 gatgggcgac gaatgctgaa tgtcacgcga agattggcca cttcgcctaa tttattggtg
    18601 gtggcgaaga ggctatccat gcaggaaaaa cctttatcta aaaaattatc tcgcatccca
    18661 gaacgggtaa ccgtaatcac gggtaacgta tcgtcaaccg tttttctgtc aatcatgtct
    18721 tttcgacaag tagccagaaa gtcttccggg ccaccataaa tgtggacacc tttatcaaga
    18781 tattccttcg ctttcttaca atctacctca acatactgac cattggcgta cataatgccc
//
LOCUS       DAJTZL010000076        18523 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.076, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000076 DAJTZL010000000
VERSION     DAJTZL010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18523
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.076"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..1710
                     /locus_tag="OO544_002266"
     CDS             <1..1710
                     /locus_tag="OO544_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HCR6767851.1"
                     /translation="EMRQTLTLHKGANYISLAQNIQQSGLLSAELQQNGQVQDSISTK
                     LSFVDNSWPVEQQKNVMLGGGENALMLPEQASNIRLQSSETPQEIFRNNLDALVDGPW
                     GGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAGPGARFTWWGED
                     GNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQADNMPLLHRALVLAWAQ
                     EMNLPCKTLLKGLDEAIARRGTKDEDFSEEDIRDINDSLILDTPESPLADAVANVLTM
                     TLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQTAAILSGLTAEQ
                     STIERALAMNWLAKYMATMPPVVLPAPAGAWAKHKLTGGGEDWRWVGQGVPDILSFGD
                     ELSPQNVQVRWREPAKTAQQSNIPVTVERQLYRLIPGEEEMSFTLQPVTSNEIDSDAL
                     YLDEITLTSEQDAVLRYGQVEVPLPPGADVERTTWGISVNKPNAAKQQGQLLEKARNE
                     MGELAYMVPVKELTGTVTFRHLLRFSQKGQFVLPPARYVRSYAPAQQSVAAGSEWTGM
                     QVK"
     gene            1711..3360
                     /locus_tag="OO544_002267"
     CDS             1711..3360
                     /locus_tag="OO544_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HCR6767852.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPGVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QDLIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            3365..4141
                     /locus_tag="OO544_002268"
     CDS             3365..4141
                     /locus_tag="OO544_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HCR6767853.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTSGIGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(4215..5399)
                     /gene="atoB"
                     /locus_tag="OO544_002269"
     CDS             complement(4215..5399)
                     /gene="atoB"
                     /locus_tag="OO544_002269"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HCR6767854.1"
                     /translation="MKNCVIVSAVRTAIGSFNGSLASTSAIDLGATVIKAAIERAKID
                     SLHIDEVIMGNVLQAGLGQNPARQALLKSGLAETVCGFTVNKVCGSGLKSVALAAQAI
                     QAGQAKSIVAGGMENMSLAPYLLDAKARSGYRLGDGQVYDVILRDGLMCATHGYHMGI
                     TAENVAKEYGITREMQDELALHSQRKAAAAIESGAFTAEIVPVNVVTRKKTFVFSQDE
                     FPKANSTAEALGALRPAFDKAGTVTAGNASGINDGAAALVIMEESAALAAGLTPLARI
                     KSYASGGVPPALMGMGPVPATQKALQLAGLQLADIDLIEANEAFAAQFLAVGKTLGFD
                     SEKVNVNGGAIALGHPIGASGARILVTLLHAMQARDKTLGLATLCIGGGQGIAMVIER
                     LN"
     gene            complement(5430..6752)
                     /locus_tag="OO544_002270"
     CDS             complement(5430..6752)
                     /locus_tag="OO544_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HCR6767855.1"
                     /translation="MIGRISRFMTRFVSRWLPDPLIFAMLLTLLTFVIALWLTPQTPI
                     SMVKMWGDGFWNLLAFGMQMALIIVTGHALASSAPVKSLLRTAASAAKTPVQGVMLVT
                     FFGSVACVINWGFGLVVGAMFAREVARRVPGSDYPLLIACAYIGFLTWGGGFSGSMPL
                     LAATPGNPVEHIAGLIPVGDTLFSGFNIFITVTLIVVMPFITRMMMPKPSDVVSIDPE
                     LLMEEADFQKQLPKDAPPSERLEESRILTLIIGALGIAYLAMYFSEHGFNITINTVNL
                     MFMIAGLLLHKTPMAYMRAISAAARSTAGILVQFPFYAGIQLMMEHSGLGGLITEFFI
                     NVANKDTFPVMTFFSSALINFAVPSGGGHWVIQGPFVIPAAQALGADLGKSVMAIAYG
                     EQWMNMEQPFWALPALAIAGLGVRDIMGYCITALLFSGVIFVIGLTLF"
     gene            complement(6749..7399)
                     /gene="atoA"
                     /locus_tag="OO544_002271"
     CDS             complement(6749..7399)
                     /gene="atoA"
                     /locus_tag="OO544_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit beta"
                     /protein_id="HCR6767856.1"
                     /translation="MDAKQRIARRVAQELRDGDIVNLGIGLPTMVANYLPEGIHITLQ
                     SENGFLGLGPVTTAHPDLVNAGGQPCGVLPGAAMFDSAMSFALIRGGHIDACVLGGLQ
                     VDEEANLANWVVPGKMVPGMGGAMDLVTGSRKVIIAMEHCAKDGSAKILRSCTMPLTA
                     QHAVHMLVTELAVFRFIDGKMWLTEIADGCDLATVRAKTEARFEVAADLNTQRGDL"
     gene            complement(7399..8061)
                     /gene="atoD"
                     /locus_tag="OO544_002272"
     CDS             complement(7399..8061)
                     /gene="atoD"
                     /locus_tag="OO544_002272"
                     /EC_number="2.8.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit alpha"
                     /protein_id="HCR6767857.1"
                     /translation="MKTKLMTLQDATGFFRDGMTIMVGGFMGIGTPSRLVEALLESGV
                     RDLTLIANDTAFVDTGIGPLIVNGRVRKVIASHIGTNPETGRRMISGEMDVVLVPQGT
                     LIEQIRCGGAGLGGFLTPTGVGTVVEEGKQTLTLDGKTWLLERPLRADLALIRAHRCD
                     TLGNLTYQLSARNFNPLIALAADITLVEPDELVETGELQPDHIVTPGAVIDHIIVSQE
                     SK"
     gene            complement(8257..9642)
                     /gene="atoC"
                     /locus_tag="OO544_002273"
     CDS             complement(8257..9642)
                     /gene="atoC"
                     /locus_tag="OO544_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate metabolism transcriptional
                     regulator AtoC"
                     /protein_id="HCR6767858.1"
                     /translation="MTAINRILIVDDEDNVRRMLSTAFALQGFETHCANNGRTALHLF
                     ADIHPDVVLMDIRMPEMDGIKALKEMRSHETRTPVILMTAYAEVETAVEALRCGAFDY
                     VIKPFDLDELNLIVQRALQLQSMKKEIRHLHQALSASWQWGHILTNSPAMMDICKDTA
                     KIALSQASVLISGESGTGKELIARAIHYNSRRAKGPFIKVNCAALPESLLESELFGHE
                     KGAFTGAQTLRQGLFERANEGTLLLDEIGEMPLVLQAKLLRILQEREFERIGGHQTIK
                     VDIRIIAATNRDLQAMVKEGTFREDLFYRLNVIHLILPPLRDRREDISLLANHFLQKF
                     SSENQRDIIDIDPMAMSLLTAWSWPGNIRELSNVIERAVVMNSGPIIFSEDLPPQIRQ
                     PVCNAGEVKTASVGERNLKEEIKRVEKRIIMEVLEQQEGNRTRTALMLGISRRALMYK
                     LQEYGIDPADV"
     gene            complement(9639..11465)
                     /gene="atoS"
                     /locus_tag="OO544_002274"
     CDS             complement(9639..11465)
                     /gene="atoS"
                     /locus_tag="OO544_002274"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     AtoS"
                     /protein_id="HCR6767859.1"
                     /translation="MHYMKWIYPRRLRNQMILMAILMVIVPTLTIGYIVETEGRSAVL
                     SEKEKKLSAVVNLLNQALGDRYDLYIDLPREERIRALNAELAPITENITHAFPGIGAG
                     YYNKMLDAIITYAPSALYQNNVGVTIAADHPGREVMRTNTPLVYSGRQVRGDILNSML
                     PIERNGEILGYIWANELTEDIRRQAWKMDVRIIIVLTADLLISLLLIVLFSRRLSANI
                     DIITDGLSTLAQNIPTRLPQLPGEMGQISQSVNNLAQALRETRTLNDLIIENAADGVI
                     AIDRQGDVTTMNPAAEVITGYQRHELVGQPYSMLFDNTQFYSPVLDTLEHGTEHVALE
                     ISFPGRDRTIELSVTTSRIHNTHGEMIGALVIFSDLTARKETQRRMAQAERLATLGEL
                     MAGVAHEVRNPLTAIRGYVQILRQQTSDPIHQEYLSVVLKEIDSINKVIQQLLEFSRP
                     RHSQWQQVSLNALVEETLVLVQTAGVQARVDFISELDNELSPINADRELLKQVLLNIL
                     INAVQAISARGKIRIQTWQYSDSQQAISIEDNGCGIDLSLQKKIFDPFFTTKASGTGL
                     GLALSQRIINAHQGDIRVASLPGYGATFTLILPINPQGNQTV"
     gene            11632..14481
                     /gene="rcsC"
                     /locus_tag="OO544_002275"
     CDS             11632..14481
                     /gene="rcsC"
                     /locus_tag="OO544_002275"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HCR6767860.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            complement(14681..15331)
                     /gene="rcsB"
                     /locus_tag="OO544_002276"
     CDS             complement(14681..15331)
                     /gene="rcsB"
                     /locus_tag="OO544_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsB"
                     /protein_id="HCR6767861.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(15348..18020)
                     /gene="rcsD"
                     /locus_tag="OO544_002277"
     CDS             complement(15348..18020)
                     /gene="rcsD"
                     /locus_tag="OO544_002277"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HCR6767862.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDTTATGDNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESLPNTAVNNELRILRAINEKIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAAGPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
BASE COUNT         4526 a         4535 c         4867 g         4595 t
ORIGIN      
        1 gagatgcgcc agacgctgac gctgcacaaa ggggcgaatt atatttcgct ggcgcagaac
       61 attcagcaat ctggcttgtt aagcgcagaa ctgcaacaaa atgggcaagt gcaggacagc
      121 attagcacaa aactgtcttt tgtggataac agctggcccg ttgaacagca gaaaaatgtc
      181 atgctcggcg gtggcgagaa cgcgctgatg ttgcccgagc aggctagcaa tatccggcta
      241 caaagtagcg aaacgccgca ggagattttc cgcaacaatc ttgatgcgtt agtcgatgga
      301 ccatggggtg gcgtgatcaa caccggtagc cgtctgatcc cgctcagtct cgcctggcgt
      361 tcgcttgccg atcatcaaag tgccgccgct aacgacattc gtcagatgat tcaggataac
      421 cgtctgcggc tgatgcaact ggcggggccc ggagcgcgct ttacctggtg gggtgaagat
      481 ggcaatggtg acgccttcct tacggcatgg gcatggtacg ccgactggca ggccagccag
      541 gcgctcggcg taacgcaaca accggaatac tggcagcata tgctcgacag ttatgccgag
      601 caggcggata acatgccgtt attgcatcgg gcgctggtgc tggcgtgggc acaggagatg
      661 aatctgccgt gcaaaacgtt gttgaaaggg ttggatgaag ctatcgcccg gcgcggaact
      721 aaagatgaag atttctctga ggaagacatc cgcgatatca atgacagcct gatcctcgat
      781 acaccggaat ctccactggc agatgcggtg gcaaacgtct taaccatgac gttgctgaaa
      841 aaagcgcagt tgaagtccac ggtgatgcca caggttcagc aatatgcgtg ggataaagcg
      901 gcaaacagca atcagccgct ggcgcacacg gttgtgctgc tcaatagcgg tggtgacgct
      961 acccagacgg ccgctatttt aagtggtttg accgctgagc aatccactat tgagcgcgcg
     1021 ctggccatga actggctggc gaaatatatg gcgacaatgc ctccagttgt tttgcctgcg
     1081 cctgcgggcg catgggctaa acataagtta actggagggg gcgaagactg gcgttgggtt
     1141 ggtcagggtg tgccggacat tctctctttt ggtgacgaat tatcgccgca aaatgtgcag
     1201 gtccgctggc gtgaaccggc aaaaacggct caacaaagta acattccggt gaccgttgaa
     1261 cgccagttgt atcggcttat ccccggtgaa gaagagatga gctttactct gcagccggtg
     1321 accagcaatg agattgacag cgatgcgctg tatctcgatg aaattacgct taccagcgag
     1381 caggatgcag ttctgcgcta tggtcaggtg gaagtaccgc tcccgccggg agccgacgtt
     1441 gagcgcacaa catggggcat ttcagtcaat aaacccaacg ccgcgaaaca gcaggggcaa
     1501 ttgctggaaa aagcgcgtaa tgaaatgggc gaactggcct atatggtgcc ggtgaaagaa
     1561 ctgacgggaa cggtcacttt ccgccatttg ctgcgcttct cgcaaaaagg gcaattcgtt
     1621 ctgcctcctg ctcgttatgt gcgttcctat gcacctgcgc agcaaagtgt tgcggcaggg
     1681 agtgaatgga ccgggatgca ggtgaaataa gtgaactggc gcagaatcgt ctggctattg
     1741 gcgttggtta ctctgccaac gcttgcggag gagacgccgt tacaactggt gctgcgcggt
     1801 gcgcagcacg atcaactgta tcagctgtcg tcatcaggcg tgaccaaagt ctcagcatta
     1861 cctgactcgc tcaccacgcc gttagggagt ttgtggaaac tgtatgttta cgcctggctg
     1921 gaggataccc atcaaccgga gcagccctac cagtgtcgcg gaaactcccc ggaagaggtt
     1981 tattgttgtc aggctgggga gagcataacg cgcgataccg cgctggtacg ttcctgtggg
     2041 ctctattttg cgccgcagcg gttgcatatt ggcgcagatg tgtgggggca atactggcaa
     2101 cagcgtcagg cacctgcgtg gttagcgtct cttacaacgc tgaagccaga aacctcggta
     2161 acggttaaat cgctgctcga ttcattagcg acgttacctg cgcaaaacaa agctcaggaa
     2221 gtcttactgg atgtggtgct ggacgaggcg aaaatcggtg tcgcatccat gctcggtagt
     2281 cgggtacggg tgaaaacctg gagctggttt gccgatgata aacaggaaat ccgccagggt
     2341 ggttttgctg gctggttaac cgatggtaca ccgctgtggg ttactggcag tggcaccagc
     2401 aaaacggtac taacccgtta tgcgacggtg cttaatcggg ttttgccagt gccgacgcag
     2461 gttgccagtg ggcaatgtgt agaggtggag ctgtttgccc gctatccgct gaagaaaatc
     2521 acagcggaaa agagtacgac ggcagtaaaa cccggcgtac ttaatgggcg ctatcgtgtg
     2581 acgtttacta atggtaacca cattacgttt gtcagccacg gtgagacgac cttactgagc
     2641 gaaaagggca aactgaaatt gcagtcgcat ctcgatcgtg aagagtacgt tgccagagtt
     2701 ctggatcgcg aggcgaaaag tacgccacct gaggcagcga aagccatgac cgtggcgata
     2761 cgtacttttt tacagcaaaa cgccaatcgg gaaggtgatt gcctgaccat ccccgacagc
     2821 agcgccacgc agcgtgtttc tgcttcgcca gcgaccacgg gggcgcgaac gatgaccgct
     2881 tggacgcagg atctcatcta tgcaggcgat ccggttcatt atcatggcag tcgcgctacc
     2941 gaagggacac tttcctggcg gcaagccacg gcccaggcgg ggcaggggga gcgttacgac
     3001 cagatactcg cgtttgctta tcccgacaac agccttagcc gctggggagc tccacgctca
     3061 acctgccagt tattacccaa agcgaaagcc tggctggcga aaaaaatgcc gcagtggcgg
     3121 cgtatattac aaggcgagac ggggtacaac gaaccagacg tgtttgcggt ctgtcgttta
     3181 gtctcaggtt tcccctatac cgatcggcag cagaaacggc tgtttattcg taacttcttt
     3241 acgcttcagg atcggctgga tttaacccat gagtatctgc acctggcctt tgatggttat
     3301 cccaccgggc ttgatgagaa ctatatcgaa acgctgaccc gacaattatt aatggactaa
     3361 tgctatgcga aagatttttc ttccgttgtt actggtggca ctttcgcctg tcgctcacag
     3421 tgaaggtgtg caggaagtcg agattgacgc accgctctct ggctggcatc cggcagaggg
     3481 cgaagatgcc agtttttcac aatcgatcaa ttacccggcg tcgtccgtca acatggcaga
     3541 tgatcaaaat atctcagcgc agatccgcgg caaaataaag aattatgctg cggcgggtaa
     3601 agttcagcag ggccggctgg tggtcaacgg tgccagtatg ccgcagagaa ttgaatccga
     3661 tggttcattt gcacgccctt atattttcac tgaaggcagc aacagcgtgc aggtcatcag
     3721 cccggatggg caaagccggc aaaaaatgca gttttactca acgtcgggta taggaacgat
     3781 tcgtgcacgt ttacggctgg ttctctcgtg ggatacggac aataccgatc tcgatctcca
     3841 tgttgtcacg cccgatggcg aacacgcctg gtacggtaac accgtgctga aaaacagtgg
     3901 tgcactggat atggatgtca cgacggggta cggacccgag attttcgcca tgccagcacc
     3961 agttcacggg cgctatcagg tgtatatcaa ctactatggt gggcgcagcg aaacggaatt
     4021 gacgaccgcc caactgacgc tgatcaccga tgaaggatcg gtcaatgaga aacaggaaac
     4081 atttattgta ccgatgcgta atgctggcga gctgacgctg gtgaaaagtt ttgactggtg
     4141 attaatgggg ttaccttcgc cgtcgatgtt caagaaaacg cccgataact ttcgctatcg
     4201 ggtgttttta ttgattaatt caaccgttca atcaccatcg caattccctg gccgccacca
     4261 atgcacagtg ttgccagccc cagcgtttta tcgcgtgcct gcatggcatg taatagtgtg
     4321 accagaatac gagcaccact ggcaccgata ggatgcccga gcgcgatggc cccgccgttg
     4381 acattcactt tctcagaatc aaagcccagg gttttcccaa cggcgaggaa ctgtgcagca
     4441 aatgcttcat tagcttcgat gagatcaatg tctgccagtt gcagccccgc cagttgtaac
     4501 gctttttgcg tggcaggtac tggccccata cccatcaatg cgggaggcac gccaccgctg
     4561 gcataacttt taatgcgagc caggggggta aggcctgctg ccagcgccgc agattcttcc
     4621 ataatcacca gagcggcagc accgtcgtta ataccagacg cgttcccagc ggtgactgtt
     4681 cctgctttat cgaaggccgg gcgcaatgca cctaacgctt cagccgttga attcgctttc
     4741 gggaattcgt cttgactgaa gacgaaggtt ttcttccgag tgacaacatt taccgggacg
     4801 atttcggctg taaaagcacc ggactcaatt gcggctgccg ctttacgctg tgaatgtagc
     4861 gccagttcat cctgcatttc acgggtaatt ccgtactctt tagccacgtt ttcggcggta
     4921 atccccatat gataaccatg ggtggcgcac atcaggccat cgcgcaggat tacgtcataa
     4981 acctgtccgt ctccaagacg ataaccagag cgtgcttttg catcgagtaa gtagggcgct
     5041 aaactcatat tttccatacc ccccgccaca atgctcttcg cctgacctgc ctgaattgcc
     5101 tgggcggcaa gcgccacact tttaagaccc gaaccacaca ctttattgac cgtgaatccg
     5161 cacaccgttt ctgccagccc gctttttaac agtgcctgac gtgccggatt ttgccccaac
     5221 ccggcttgca acacgttacc cataatcact tcatcaatgt gtagtgaatc gatttttgca
     5281 cgttcaatgg cggctttaat tactgtcgcc cccaggtcga tggcgctggt ggaagcgagt
     5341 gaaccgttaa aactaccgat agcagtacgt accgcactga cgatgacaca atttttcatt
     5401 ttatattcct tctgtctgta gggttgccgt cagaacagcg ttaaaccaat gacgaaaatg
     5461 acgccggaga agagcagggc agtgatgcaa tagcccatga tgtcgcggac accgagtccg
     5521 gcgattgcca gtgctggcag cgcccagaat ggttgttcca tgttcatcca ttgctcgccg
     5581 taggcgatcg ccattaccga tttaccgaga tcagcgccca gcgcctgggc tgcgggtatc
     5641 acgaaaggtc cctgaataac ccagtgaccg ccgccagacg gaacggcgaa gttaatcagt
     5701 gcagaactaa aaaaggtcat taccgggaag gtgtctttgt tcgcaacatt gatgaagaat
     5761 tcggtaatga gtccacccag accagaatgc tccatcatca gttggatccc agcgtagaag
     5821 gggaattgca ccagaatacc ggcagtactg cgtgctgccg cgctgatagc acgcatataa
     5881 gccattggcg ttttatgtag caacagaccc gcaatcataa acatcaggtt gacggtattg
     5941 atggtgatgt tgaagccatg ttcgctgaag tacatcgcca ggtaagcgat accgagtgcg
     6001 ccgatgatca acgtcagaat gcggctttct tccagtcgct cggatggtgg ggcatctttc
     6061 ggtagctgct tttgaaaatc agcctcttcc atgagtagtt ccggatcgat actcaccacg
     6121 tcagacggtt ttggcatcat catgcgggtg ataaatggca tcaccacaat caacgtcaca
     6181 gtgatgaaaa tgttaaaacc actgaacaga gtatcgccca ccgggatcag cccggcgata
     6241 tgctcaaccg ggttgcccgg tgttgcagcc aacagaggca ttgatccaga gaagccgcca
     6301 ccccaggtga gaaaaccaat gtaggcgcag gcaatgagca acggataatc agaaccgggg
     6361 actcgccggg cgacttcacg ggcaaacatt gcgccgacaa ccaaaccaaa tccccagttg
     6421 atgacacaag cgactgaacc gaagaaagtg accagcatga cgccctgtac gggcgtcttt
     6481 gcggcggagg cggcagtacg cagcaaactt ttcaccggag cagagctggc aagggcatga
     6541 ccggtaacga tgataagcgc catctgcata ccaaacgcca gcaagttcca gaaaccgtca
     6601 ccccacattt tcaccatgct gatcggcgtt tgtggtgtta accaaagcgc gatcacgaat
     6661 gttagcaatg tcagcaacat ggcaaagatc agtggatcgg gaagccatcg gctgacaaaa
     6721 cgcgtcataa aacgcgatat gcgaccaatc ataaatcacc ccgttgcgta ttcagatcgg
     6781 cggcgacttc aaaccgagct tctgttttgg cacgcacggt ggctaaatca cacccgtcgg
     6841 caatttcggt gagccacatt ttgccgtcaa taaaacgaaa gacagccagt tcagtaacca
     6901 gcatatgcac cgcatgttgc gcagtgagtg gcatggtgca gctgcgcaaa atttttgctg
     6961 aaccatcttt ggcgcaatgt tccatggcga tgatcacttt gcgcgacccg gtcaccagat
     7021 ccatcgcgcc acccataccg ggcaccattt tcccaggcac tacccagttc gcgaggtttg
     7081 cttcttcgtc tacttgcaaa ccgccgagca cgcaggcatc aatatgaccg ccacggatta
     7141 gcgcaaatga catggcgcta tcaaacatgg ctgcaccggg taaaacaccg cacggttgcc
     7201 cgccagcgtt caccagatct ggatgcgctg tcgtgaccgg gcctaaaccg aggaagccgt
     7261 tttccgattg cagagtgata tgaataccct ccggtaaata attggcgacc attgtgggta
     7321 aaccgatccc taagttaacg atgtcaccat cacgaagctc ttgcgccaca cggcgcgcaa
     7381 tacgttgttt cgcatccatt atttgctctc ctgtgaaacg atgatgtggt cgataacggc
     7441 accaggggtg acaatatggt caggttgcag ctcgccggtt tcgaccagtt catctggctc
     7501 taccagcgtg atatcagccg caagggctat cagggggtta aagttgcggg cgctaagttg
     7561 ataggtcagg ttgccaagtg tgtcgcaacg atgagcgcga attagcgcca ggtcggcgcg
     7621 cagtgggcgt tcgagcagcc aggttttacc gtcgagtgtc agtgtctgtt tgccttcctc
     7681 tacgacggtg ccgacacccg ttggggtgag aaaaccacca agtccagctc caccacagcg
     7741 aatttgctcg attagcgtac cttgcggcac cagaacgacg tccatctcac cagatatcat
     7801 gcgccgacct gtttccgggt tggtgccgat atgtgaagca atcactttgc ggactcgacc
     7861 attgacgatg agcggaccga tgccggtatc aacaaacgcg gtatcattgg ctatcaatgt
     7921 caggtcgcga acaccagatt ccagtaatgc ttcaaccagg cgggatggag tgccaatccc
     7981 cataaatccg cccaccatga tggtcatgcc gtcacgaaag aagccggtgg cgtcttgtaa
     8041 tgtcatcaat tttgttttca ttttttatcc ctcttgcata caccgttgtg ggtacagtca
     8101 ggcaatagca aggggagtgc cagaatgaga ttattggatt tagttattta actaattgaa
     8161 tatgaaggga taaattggat ggggatttat aaggagaatg agcggctatg cagaaaattg
     8221 cgcactgtgc aaatttctgc atagcaagtt ttggtgttat acatccgccg gatcgatacc
     8281 gtattcctgg agtttataca tcaatgcacg gcgactgatg cccagcatta aagcagtgcg
     8341 ggttcggttt ccttcttgtt gttccagcac ttccataatg atgcgttttt cgacgcgttt
     8401 aatttcctct tttaaattac gctcaccgac agaggctgtt tttacctcgc cagcattaca
     8461 gactggctga cgaatctgtg gcggaagatc ctcagaaaaa atgatcgggc ctgaattcat
     8521 cacgacggcg cgttcaataa cgttagaaag ctctcgaata tttcccggcc atgaccaggc
     8581 ggtaagcagt gacattgcca tcggatcgat gtcgataata tcgcgctgat tctcactact
     8641 gaatttttgc aaaaagtgat tagctaacag ggaaatatct tcccggcgat cgcgcagagg
     8701 cggcagtatt aaatgaataa cgttaaggcg ataaaagaga tcttcacgga aggtgccttc
     8761 ttttaccatt gcctgcaagt cacggttggt ggcagcaatg atgcggatat caacttttat
     8821 ggtctgatgg ccgccaatcc gttcaaattc ccgttcctgt agaatgcgta gtaatttggc
     8881 ttgtagtacc agcggcattt cgccaatttc gtcgaggagc agagtacctt cgttggctcg
     8941 ttcaaataat ccctgacgca aggtttgtgc accagtaaat gcaccttttt catgaccaaa
     9001 cagttcactt tcgagcaacg attccggcag cgccgcgcag ttgactttaa tgaacggccc
     9061 ctttgcccgc cgcgaattgt agtgaatcgc tctggcaatc aactctttcc cggtgccgct
     9121 ttcaccgcta atcaagacgc tggcctgaga aagggcaatt ttggcggtgt ctttgcagat
     9181 gtccatcatc gccgggctgt tggtgagaat gtgcccccat tgccagctgg cgctcagtgc
     9241 ctggtgcaga tgacggatct cttttttcat tgactggagt tgtaaagcgc gctgaacgat
     9301 taaattcaac tcatcgagat caaacggttt aataacatag tcgaaggctc cgcagcgtag
     9361 cgcttcgacg gcggtttcca cttccgcata ggccgtcatc agaataacgg gtgtccgggt
     9421 ctcatggctg cgcatctcct ttagtgcctt gatgccgtcc atctctggca tgcggatatc
     9481 catcaacacc acatcagggt gaatatcggc aaacaggtgt aatgctgtgc gtccgttgtt
     9541 cgcacaatgt gtttcgaatc cttgtagtgc aaaagcggtg ctcagcatac ggcgaacatt
     9601 atcttcatca tccacaataa ggatgcgatt aatagcagtc atacagtctg atttccctgc
     9661 gggttgatcg gtaaaataag cgtgaaggtt gcgccgtagc ccggcaaact ggcgacgcga
     9721 atatcaccct gatgggcatt aatgatgcgt tgacttaacg ccagaccaag cccggttcct
     9781 gaggctttgg tggtgaaaaa gggatcgaag atcttttttt gcagcgagag atcaatgcca
     9841 cagccgttgt cctctatcga aatggcctgt tgtgagtcgc tgtattgcca ggtttgaatg
     9901 cgaattttcc ctcgtgcgct gatagcctgg acggcattga tcaggatatt cagtagtacc
     9961 tgtttgagca gttcacgatc ggcgttaatc gggcttaatt cattatccag ttcgcttatg
    10021 aagtcgaccc gcgcttgtac gccggcggtt tgtaccagta ccagagtttc ttcaaccaat
    10081 gcattgaggc tgacttgttg ccattgactg tggcgtggac gtgaaaattc gagcaattgc
    10141 tgaataactt tgttaattga atcgatttct ttgagtacta cggacagata ttcctgatgt
    10201 attgggtcac tggtttgttg gcgcaagatc tgtacataac cacgaatagc cgttaacgga
    10261 ttacgtactt catgcgcgac gccagccatc agctcaccca gtgtggcgag gcgttctgct
    10321 tgcgccatgc ggcgctgggt ttctttgcgg gcagttaaat cagagaaaat caccaaagca
    10381 cctatcattt caccgtgcgt gttatgaata cgactggtag tgacactgag ttcaatggtg
    10441 cggtcacgac ctggaaaact gatctccagc gccacatgtt cggtgccatg ttccagcgta
    10501 tccagtactg gactgtagaa ctgagtattg tcgaacaaca tggagtaagg ctgccctacc
    10561 agttcatggc gttgatagcc agtgataact tctgctgctg ggttcatggt ggttacatca
    10621 ccctggcggt caatggcaat gacgccatcg gcagcgtttt caataatcag atcgttaagt
    10681 gtccgcgttt cacgcagtgc ctgggcgagg ttattaacac tctgactgat ttgccccatt
    10741 tcaccgggca attgtggtaa tcgagtggga atattttgtg ccagagtcga gaggccatcg
    10801 gtgatgatat caatattggc gctcaggcga cgggagaaaa ggacaatcaa tagcaggctt
    10861 atcagcaaat cggcggtgag cacaatgata atcctcacat ccattttcca ggcctggcgg
    10921 cgaatatctt cggttaattc attggcccag atatagccga ggatttcacc attacgctca
    10981 atggggagca ttgaattcaa aatatcgccg cgcacctgcc tgcctgaata aaccaaaggg
    11041 gtatttgtac gcatgacttc gcgaccaggg tgatctgcgg caatggtaac gccgacatta
    11101 ttctgatata gcgctgaagg cgcgtaggtg attatcgcat ccagcatttt gttgtaataa
    11161 ccagcaccta tgccagggaa ggcgtgagtg atattttcgg taatgggggc aagttctgca
    11221 tttaatgcgc ggatacgctc ctcacgtggt aagtcgatgt agagatcata gcgatcgcct
    11281 agtgcctgat taagcaggtt gaccacggca gaaagttttt tctctttttc agataagact
    11341 gctgaacgtc cttccgtttc tacgatataa ccaatagtaa gcgttgggac aatgaccatc
    11401 aggattgcca tcaggatcat ttgattgcgt aagcggcgtg gataaatcca cttcatataa
    11461 tgcatgctga aatccactag tcttgtccgg tatatgacga ttatcagagg ttaaggtgat
    11521 gatttctcgg cggtgtatca tattccagag aagagagaac attgcggtaa cacgctttta
    11581 ccgctacctt aaccacactc catcggtcac ctgaggcgga gcttcgcccc tttgaaatac
    11641 cttgcttctt ttcgtacaac cctgaaagcc tcgcgctaca tgttcagagc attggcgtta
    11701 gtgctctggc tgttgattgc tttttcatcc gttttttaca tcgttaatgc gttacatcag
    11761 cgagaatcgg aaattcgtca ggaatttaat ctgagttccg atcaggctca gcgctttatt
    11821 caacgcacct ctgatgtgat gaaagagctg aagtacatcg ccgaaaatcg cttatcggca
    11881 gaaaacggtg tgctttcccc gcgtggacga gaaacgcagg cggatgtgcc tgcgtttgaa
    11941 ccgctgtttg ccgactccga ttgttccgca atgagtaaca cctggcgagg ttctctggag
    12001 tcattggcgt ggtttatgcg ctactggcgc gataattttt ctgcggctta cgatctaaac
    12061 cgggtatttt taatcggcag cgataacctc tgcatggcca atttcggtct gcgtgatatg
    12121 ccagtggaac gcgataccgc gttgaaagct ttgcatgaac gcatcaataa atatcgaaat
    12181 gcaccacaag atgatagcgg cagtaacctc tactggatca gcgaaggtcc gcgccctggc
    12241 gtcgggtatt tttacgcgtt gacgccagtt tatctggcga accggttgca ggcgcttttg
    12301 ggtgtcgagc agaccatccg gatggagaac tttttcttac cgggtacgtt gccgatgggg
    12361 gttaccattc ttgatgaaaa tggtcatacc ctgatttcgc ttaccggacc agaaagtaaa
    12421 attaagggcg atcctcgctg gatgcaggaa cgctcctggt ttggctatac ggaagggttc
    12481 cgggagctgg tgctgaagaa aaatctgcca ccctcatcgc taagcatcgt gtattcggtg
    12541 ccggttgata aggtgctgga acgcattcgc atgttgatcc ttaacgcaat tttgctgaat
    12601 gtgcttgccg gagctgcatt gtttactctc gcacggatgt acgagcgacg tattttcatt
    12661 ccggcggaaa gcgacgccct gcgactggaa gaacatgagc agttcaatcg caagattgtc
    12721 gcctccgcgc cagtgggtat ctgcattttg cgtaccgctg atggcgtcaa tattttaagt
    12781 aacgaactgg cgcataccta tctcaatatg cttacgcatg aggaccgcca acgactgacg
    12841 caaattatct gtgggcagca ggtcaatttt gttgatgtcc tgaccagcaa caataccaat
    12901 ctgcaaatca gcttcgtcca ttcgcgctat cgtaatgaaa acgtggccat ttgtgtgctg
    12961 gtggatgttt cttcgcgcgt gaagatggaa gagtcgttgc aggagatggc acaagcagcg
    13021 gaacaggcga gccagtcaaa atcgatgttc cttgccaccg tcagtcatga gctgcgaacg
    13081 ccgctgtatg gcattatcgg taacctggat ctgttgcaaa ccaaagagtt accgaaaggc
    13141 gtcgatcggc tggtgacggc aatgaacaac tcttccagcc tgttgttgaa aattatcagc
    13201 gatattctcg atttctcgaa gattgaatcg gaacagttga agatcgaacc gcgtgagttt
    13261 tcaccgcgtg aagtgatgaa ccacatcacc gccaactatt taccgctggt ggtacgcaag
    13321 cagttaggct tgtactgctt tattgaaccg gatgtgccag tggccttaaa tggcgacccg
    13381 atgcgtttac agcaggtcat ctccaacctg ttgagtaacg ccataaaatt caccgatacc
    13441 ggctgtatag ttttgcatgt ccgcgctgat ggcgattatc tctctatccg tgttcgcgat
    13501 accggcgtgg ggattccggc gaaagaagtg gtgcgcttgt ttgatccctt cttccaggtc
    13561 ggaacgggcg tacagcgaaa cttccagggg accggtctgg gtctggcgat ttgtgaaaaa
    13621 ctgatcagca tgatggacgg cgatatctcg gtagattcag aaccgggaat gggcagccag
    13681 tttaccgtgc gtattccgtt gtacggcgct cagtacccgc agaaaaaagg cgtggaaggg
    13741 ttgagtggta aacgctgctg gctggcggtc cgcaatgcgt cgctctgtca gttcctggaa
    13801 accagtttgc agcgcagcgg catcgtcgtt acaacatacg aagggcagga accgactccc
    13861 gaagatgtgt taattactga cgaggtagtg agtaaaaaat ggcagggtag agcggtagtg
    13921 accttctgtc gtcgccatat tggtattccg ctggagaaag cgccagggga gtgggtacac
    13981 agtgtggctg ccccgcatga gctaccggca ttgttggcgc gtatttattt gatcgagatg
    14041 gagagcgacg atcctgctaa cgctctgccg tcgacggaca aagcggtcag cgataatgac
    14101 gatatgatga ttctggtcgt ggatgatcat ccgattaacc gacgtctgct ggcagatcag
    14161 ttgggatcgt tgggctatca atgtaaaacc gcgaatgatg gtgtcgatgc gcttaatgta
    14221 cttagcaaga atcatattga tatcgtgctt agcgacgtca acatgccaaa tatggatggt
    14281 taccgcttga cgcaacgcat tcgtcagttg ggactgacgt tgccggtaat cggagtaact
    14341 gctaatgcgt tggctgaaga gaagcagcgg tgtctggagt ccggtatgga cagctgcctg
    14401 tcgaagccgg taacgctgga tgtgataaaa cagacgctga cggtatatgc cgagagggtc
    14461 aggaaatcgc gggaatcgta ggacggataa ggtgtttatg ccgcatctgg cattcagtgc
    14521 aaatgccaga tgcgacgctg acgcgtctta tctggcctac tttgatgtaa tctgttgaaa
    14581 taatgggaat cgtaggacgc ataaggcgtt tacgccgcat tcggcaatcg gtgcaaatgc
    14641 cagataagac actaacgcgt cttatctggc ctacaggtga ttagtcttta tctgccggac
    14701 ttaacgtcac tgaagagaga taattcagca gggcgatatc gttctcgaca cccagcttca
    14761 tcatcgcaga tttcttctgg ctactgatgg ttttaatact gcggttcagc tttttagcga
    14821 tctcggtcac caggaagcct tccgcaaaca ggcgcagaac ttcactctct tttggcgaga
    14881 gacgcttgtc accgtaacca ccagcactga ttttttccaa caggcgagaa acgctttccg
    14941 gggtaaattt cttccctttc tgcagcgcgg cgagagcttt cggcagatcg gtcggtgcac
    15001 cttgtttcag cacgatccct tcgatatcca gatccaatac cgcactaaga atcgccgggt
    15061 tgttgttcat agtcagaaca atgatcgaca ggcttgggaa atggcgcttg atgtacttga
    15121 ttaaggtaat gccatcgccg tacttatcgc caggcatgga gagatcggta atcaacacat
    15181 gcgcatccag tttcggcagg ttgttgatca gtgctgtaga gtcttcaaat tcgccgacaa
    15241 cattcaccca ctcaatttgc tcaagtgatt tgcgaatacc gaacaagact atcggatggt
    15301 catcggcaat aattacgttc atattgttca tgtaataggc taccttgcta cagcaagctc
    15361 ttgacataac tgtcaatgtc gctgatgtat ttttctattc ctggaacatc cttctcacga
    15421 atcagatgtt ccagcgtttc acataactgc ttgccgggta ccagatttag catggcaaat
    15481 acgcctttaa gacgatgagc cgtttgggct aacgcagcaa agtcactggt tgctgcttca
    15541 gtatacagcc tcttaacatc atccggtact gtgtctacaa agagcgcata atagccgctg
    15601 gcatggagtt gcgcattttc atctccgccc agaggcgatt ctgtcacctc ttcctgcgcc
    15661 agttgcactt caattaattg taagaccgct tcctgcatag cgttgctcat attgaagttg
    15721 acgcacaatt gaccaggccc aatttcccgt acgccagact catcatcgct taaaagcaag
    15781 ccagaggcag taagattaga cggattatcc gttaaaaaga tatcataatc ttgactaatt
    15841 aatctttcat cgggtgtgat acaggttgca ccccaatttt ctaactggcg agtgacaata
    15901 ttccgaattt ctgccgaagt aacatccacc attacgcaga catcatccag taaacgctct
    15961 tcttcctctt caacttccgg gccagcggcg agcattttga tatgcacaga gtagcgtgta
    16021 ccaagcccat cccgcgtttt gatgtttaaa tgaccgccca gtttacgtgc cagttgatcg
    16081 ctcagccaga atgccagcgg gtccgccttt ccataacgat cgttttgggt ctggttgata
    16141 aacgggaagt gcaaattatc catttcatga atacttacgc cttctccggt gtccagaatg
    16201 cggaatgtca ggcggtcttc agaggactca tcctgatcaa cctcaagggt gatttttccc
    16261 aattgcgttg aggtcacggc atattgcatc agtagcagca aaatacgtcg taaggcatcg
    16321 cgatcgccgc ggcgcatatc gtgtgctttc agatgattgt taatcagcag ttgcagacct
    16381 ttacgcttga tggcaggcaa cactgaaggc acaacttcat caattaaatc ctgcacggag
    16441 aacagcaccg tctcactttt ccagctatcg tccgcaagca tgttcgctaa ctgtatttca
    16501 tcgaccagcc gcaccagcac atctgcctga ttcgccagtt gtttgctttc cggggcgttg
    16561 agtttagccg cgctctccgc cagggactgt gcaggttctt tcagcgcatc gccaatgttt
    16621 ttcataaagg tcatccggcc ctgctggttt ttctcataca gacgctgcgc ctgcttgagt
    16681 ttcttgttta ccagcacttc gcgatcctga tcgcgaataa tgaaaatttg tgtgcgcggc
    16741 gcgacctggc tgcggaacat gcggatctca tacagctcgt tattgatcgt cgcctgaata
    16801 atcccctgat gctgttccgc catggtggtg atgttttgca gattcaaatg cggcagcaaa
    16861 tgatcggcaa ttttgttact tatgacagtg cggttcgatt cctgatcgtg aaccagcagg
    16921 ccgagcggca gcagtgagac tatcttttca ttgattgccc gtaaaatgcg caattcgtta
    16981 ttgaccgccg tgttgggtag actttctgta ctgcgactgg agaaatggcg gaatgtggta
    17041 tagccaaata acgccagcgc cagcaaaccg atgttcagca gcagtggcag cagaatgttt
    17101 tgcaacgtat ccagcaataa ggtgccataa ggaacctgcc agaccaggcg catatcggta
    17161 gagttgagtg ccgaggagat ttcaatcttc gtactgttaa agtggatact gacgctatcc
    17221 gttccttctt tctcattatc attgtctccc gttgccgtcg tgtctggctc aaggcggaaa
    17281 ctgtccagcg gcatacccgg tgggatcaaa tcattaatcg gcagatcaaa agccacgacc
    17341 gttgccagat gtcccggctg gttgaacgtg gtacgcaagg taaagtaatg accgttctgc
    17401 caggccaggc ggcgcatgtt agaaaaactt tcgcgttcat cgagggcgtt ggcctgttgc
    17461 aacatttctg cacgacgtga atcaacaatg tcactgacgg tcgattcttt aaatccggag
    17521 gtgagatctt tgaggggcag ggttgagatc agtaccagac tgttatcctg accattcagg
    17581 taatacatcg accacggtac attttctgca ccccacaatg tatccagata agtggacatc
    17641 cgctgagtca tctcaagcgt tgagttgtcg tgagagccaa agattaacgc ttccgttttg
    17701 cggcgtggtt tctccagata gtagacatcc tgtttcaggc gcgtctcttg taaaccttcg
    17761 ccggaggagg gggaggtcgt cgcggcaatg ttgtcgtaga tctgccaggt cacgtaacgc
    17821 caggtatcga cgcgcttttg gattgcgtgg gtaatgtcga caatctggta acttttatct
    17881 ttcagccagg cgttaacggc gctttgtacc attaccccca tcgtcaccag taacacaatg
    17941 atcaacagta aaaagaagcg ggtaatgctc cccggtagga gtgaaaagcg ggtcgtggcc
    18001 gttgtctctt tctgacgcat tcgtgtttat gacctgttaa aacttcgcga agtgataggg
    18061 cagtataaag ggtacagtgt gaaattccag actcttacct gattgatgat tctctattct
    18121 cccgtgatat ttatcatcct gcattggggg aaatagatgt acaagcgcca ttttgctgta
    18181 caaataacct acaaaaagcc caacgaaatt aattgcaaat aaacgaagat tgctggaaat
    18241 tatgctgatg ttaattattt gtgaaatagt tgacaagagt tatagttttt ctgtggtagc
    18301 acagaataat gaaagtgtgt aaagaagggt aaaaaaaacc ggatgcgagg catccggttg
    18361 aaataggggt aaacagacat tcagaaatga atgacggtaa taaataaagt taatgatgat
    18421 agcgggagat attctagttg cgagtgaagg ttttgttttg acattcagtg ctgtcaaata
    18481 cttaagaata agttattgat tttaaccttg aattattatt gct
//
LOCUS       DAJTZL010000077        18513 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.077, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000077 DAJTZL010000000
VERSION     DAJTZL010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18513
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.077"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            76..288
                     /gene="cspH"
                     /locus_tag="OO544_002278"
     CDS             76..288
                     /gene="cspH"
                     /locus_tag="OO544_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HCR6767863.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            735..1040
                     /locus_tag="OO544_002279"
     CDS             735..1040
                     /locus_tag="OO544_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6767864.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAASEATNGTEATTGTTGTTTTTTG
                     ATTTATTTGGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            1147..1791
                     /gene="gfcB"
                     /locus_tag="OO544_002280"
     CDS             1147..1791
                     /gene="gfcB"
                     /locus_tag="OO544_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HCR6767865.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNSGQDPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGRNV
                     LCNVWQEEVTSTRPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            1788..2534
                     /locus_tag="OO544_002281"
     CDS             1788..2534
                     /locus_tag="OO544_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HCR6767866.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPGEQQTLSVGPMEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            2534..4630
                     /locus_tag="OO544_002282"
     CDS             2534..4630
                     /locus_tag="OO544_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HCR6767867.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAYQPAPESEGLQYTTVANQLTALKYNAGFEAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGQSEQLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLYPMTAEREVPVGQ"
     gene            4676..5815
                     /locus_tag="OO544_002283"
     CDS             4676..5815
                     /locus_tag="OO544_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HCR6767868.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            5803..6249
                     /gene="etp"
                     /locus_tag="OO544_002284"
                     /gene_synonym="yccY"
     CDS             5803..6249
                     /gene="etp"
                     /locus_tag="OO544_002284"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HCR6767869.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     IKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            6269..8449
                     /gene="etk"
                     /locus_tag="OO544_002285"
     CDS             6269..8449
                     /gene="etk"
                     /locus_tag="OO544_002285"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HCR6767870.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDIVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVGAVLARAMLR
                     RGVEAPEQLEEHGISVYATIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSDAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(8564..9862)
                     /gene="appA"
                     /locus_tag="OO544_002286"
     CDS             complement(8564..9862)
                     /gene="appA"
                     /locus_tag="OO544_002286"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HCR6767871.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     GQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILSRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLKREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIKTALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(9942..10034)
                     /gene="cbdX"
                     /locus_tag="OO544_002287"
     CDS             complement(9942..10034)
                     /gene="cbdX"
                     /locus_tag="OO544_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HCR6767872.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            complement(10047..11183)
                     /gene="appB"
                     /locus_tag="OO544_002288"
     CDS             complement(10047..11183)
                     /gene="appB"
                     /locus_tag="OO544_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCR6767873.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            complement(11195..12739)
                     /gene="appC"
                     /locus_tag="OO544_002289"
     CDS             complement(11195..12739)
                     /gene="appC"
                     /locus_tag="OO544_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HCR6767874.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKTVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLPLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            complement(12873..13730)
                     /locus_tag="OO544_002290"
     CDS             complement(12873..13730)
                     /locus_tag="OO544_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HCR6767875.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPISCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSQRQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSESEIQEAIFCGLWRVRKRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            complement(13787..14125)
                     /gene="hyaE"
                     /locus_tag="OO544_002291"
     CDS             complement(13787..14125)
                     /gene="hyaE"
                     /locus_tag="OO544_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HCR6767876.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSEFRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLREFPGYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTGGN
                     YRGVLNGIHP"
     gene            complement(14122..14709)
                     /locus_tag="OO544_002292"
     CDS             complement(14122..14709)
                     /locus_tag="OO544_002292"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HCR6767877.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQAALAQLAAWGIVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTLEEQG"
     gene            complement(14706..15412)
                     /gene="hyaC"
                     /locus_tag="OO544_002293"
                     /pseudo
     CDS             complement(14706..15412)
                     /gene="hyaC"
                     /locus_tag="OO544_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309157.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
     gene            complement(15431..17224)
                     /gene="hyaB"
                     /locus_tag="OO544_002294"
     CDS             complement(15431..17224)
                     /gene="hyaB"
                     /locus_tag="OO544_002294"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HCR6767878.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFNKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNTSPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            complement(17221..18339)
                     /gene="hyaA"
                     /locus_tag="OO544_002295"
     CDS             complement(17221..18339)
                     /gene="hyaA"
                     /locus_tag="OO544_002295"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HCR6767879.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAAWPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDCGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
BASE COUNT         4544 a         4912 c         4782 g         4275 t
ORIGIN      
        1 gcggtcttaa atatcgctgg ctttatttta ttaaccccgt atagtgcagc gcagtatttt
       61 cagtaaggaa tttgtttgtc ccgtaaaatg acaggaattg tcaaaacctt tgatcgcaaa
      121 agcggcaaag gattcattat cccctccgac ggtcgtaaag aagttcaggt ccatatttcc
      181 gcattcactc cccgcgacgc agaagtgctt ataccaggat tacgtgtgga attttgccgc
      241 gtaaatggcc tgcgaggacc aacagcggca aatgtttatc tctcttaata tgacgctgct
      301 tgtttaaagc tgactggttt tattggaata gtgcaatagt caacatcata cattataata
      361 tcagttactt ttccacaaag aaactaatcc atttaagcga atatctctgc acctaatagt
      421 tataaatgcc atttaacagc cagttatagt accacttgaa actatattac caacaataat
      481 taatagcttt cgtaacaatt tccttatctt taatttgtat tttgctaaga tttatcttat
      541 gctcacaagt cgtatttcca gaggggatcg ttaataatct tatatttccc tctgcgcacc
      601 tttcctaccc gcagtatcag tatatagtgc aagcggtatg aggaaaggga gatttcacaa
      661 ggatgttata ctcaaagtag acaatcctga tctcaagcgt acgtattgtc catgcgatga
      721 ataaaggaaa agttatgaaa cacaagcttt ctgcaatcct tatggccttc atgttgacga
      781 cgccagctgc gtttgctgcc tctgaagcaa caaacgggac cgaggcaacg acgggtacta
      841 ctggcaccac aacgaccact accggcgcaa ccacgactgc tactaccact ggtggcgtgg
      901 ctgctggcgc tgtcggcacg gcgacagttg tcggtgtggc aacggctgtt ggtgtggcga
      961 cgttggcagt tgttgcagca aatgacagtg gcgatggcgg ttctcataac acctcaacca
     1021 ccacgtccac tacacgctaa tactgcttta acgcgtcgaa attaaagtta aatgataccc
     1081 ggttgcgata ttcgtaccgg gctttagaga tttgcatttt tttaattaac ctttcaagga
     1141 ttaaaggtgc gccctcttat tttatcgatt ttcgcactat ttcttgcggg atgtacgcac
     1201 agccagcaaa gtatggtcga tacatttcgc gccagccttt tcgataatca ggatatcacc
     1261 gtagcggatc agcagatcca ggcgttgcct tattccacga tgtatttacg ccttaatgaa
     1321 gggcaacgaa tctttgtggt actgggatat atagaacaag aacaaagcaa atggttatcc
     1381 caggataacg ccatgctggt tacccacaat ggacgtcttt taaaaaccgt caaacttaat
     1441 aataatctgc tggaagtgac aaattccggg caggaccctc tgcgtaacgc gctggcaata
     1501 aaagatggca gccgctggac gcgcgatatt ctctggagtg aagacaacca ttttcgctct
     1561 gcgaccctga gttctacttt ttcctttgct ggattagaga cgctgaatat tgcgggtcgc
     1621 aatgtgctgt gtaatgtctg gcaggaagag gtgacttcca cgcggccaga aaaacagtgg
     1681 caaaacacat tctgggtcga ttcggctact ggccaggttc gtcaaagtcg gcaaatgtta
     1741 ggcgcagggg ttattcccgt agaaatgacg tttcttaaac ccgcaccatg aataaattac
     1801 agtcgtattt cattgccagc gtactttacg taatgacacc ccatgccttt gcgcaaggaa
     1861 cggtgactat ttatctgcct ggcgaacaac agacactttc cgttggcccc atggaaaatg
     1921 ttgtccagtt ggtgacacaa ccgcaactgc gcgatcgcct ctggtggcct ggtgccttgc
     1981 tgaccgactc tgcggcgaaa gctaaagcgc ttaaagacta ccaacatgtc atggcacagc
     2041 ttgcttcctg ggaagcagaa gctgacgacg atgtggcggc aacgattaaa tccgtgcgcc
     2101 agcagttact caatctcaat atcactggtc gcttaccggt caaactggac cctgattttg
     2161 tacgagtgga tgagaacagc aatcccccgt tggtcgggga ctatacgctc tataccgtac
     2221 agcgtccggt gactattact ctgctgggtg cggtttctgg ggccgggcaa ttgccctggc
     2281 aggcaggccg tagcgttacc gattatctgc aagatcaccc ccgccttgca ggcgcggaca
     2341 agaataacgt tatggtgatt acgcctgagg gtgagactgt cgtcgcccct gttgccctgt
     2401 ggaataaaag acatgtggaa ccaccgcctg gcagccagct ttggttgggc ttttctgcgc
     2461 atgtactgcc cgaaaaatat gctgatttaa atgaccagat tgtgtctgtt ttaacgcaac
     2521 gtgttcctga ctgatgaaga agaattctta tcttttaagc tgcctggcca ttgccgtctc
     2581 cagtgcctgt catgctgaag tattaaccta cccggatccg ctgggttcgt cgcaatcaga
     2641 ctttggcggc acaggattgt tgcagatgcc aaatgcgcgc atcgcaccgg aaggtgaatt
     2701 cagcgtcaac taccgggata acgatcaata ccggttctac tccacctccg tggcgctgtt
     2761 cccatggctg gaaggcacca ttcgttatac ggatgtgcgc acacgcaaat atagccagtg
     2821 ggaagatttc agcggcgatc agtcatacaa agacaaatca ttcgatttta aacttcgcct
     2881 gtgggaagaa ggttactggc taccgcaagt ggcgtttggt aaacgtgata ttgctggtac
     2941 gggtctgttt gacggtgagt atctggtggc cagcaagcaa gcggggccat ttgatttcac
     3001 cctcgggatg gcatggggct acgccggtaa tgcgggcaat attaccaacc cgttttgccg
     3061 ggtgagcgat aaatattgtc atcgcgcaga gtctcacgat gcgggcgata tcagctttag
     3121 cgatatcttt cgtggcccgg cttccatctt tggcggcatt gagtatcaaa cgccgtggaa
     3181 tcccctgcgt ctgaaactcg aatacgatgg caacaattac cagaatgatt tcgctggcaa
     3241 actgcctcag gcaagccatt tcaacgtcgg cgcagtttat cgcgctgcca gctgggcaga
     3301 tctcaacctg agttatgaac gcggtaacac gttgatgttt ggcttcacgt tacggaccaa
     3361 tttcaacgat ctgcgccctg ccctgcgcga tacgccaaaa ccggcatatc aacctgcgcc
     3421 tgaatctgaa ggattgcagt acaccacagt agcgaaccaa cttaccgcgc tgaagtacaa
     3481 cgcaggtttt gaagcaccgg aaattcagct gcgcgataag acgctgtata tgtctggtca
     3541 acaatacaaa taccgtgatt ctcgcgaagc ggtcgatcgt gccaaccgga ttctggtgaa
     3601 taacctgccg caaggcgttg agaagattag cgtgacgcaa aagcgcgagc atatggcgat
     3661 ggtgactacc gaaaccgacg tagccagcct gcgcaaacag ctggctggta cagcgcctgg
     3721 tcaatcagag caacttcaac aacaacgtgt tgaagcagaa gatctttctg cctttggtcg
     3781 gggctaccgt attcgtgaag atcgctttag ctactctttc aacccaacac tttcacagtc
     3841 gctgggcggc ccggaagatt tctatatgtt ccagctgggg ctgatgtcca gtgcccgcta
     3901 ctggtttacc gaccacctgc tgcttgatgg cggtattttc accaatattt acaacaacta
     3961 cgacaagttt aagtcttcgc tgttgcccgc ggactctacc ctgccccgcg tgcgcacgca
     4021 tatccgtgat tacgttcgca atgacgttta tctcaacaac ttgcaggcga actactttgc
     4081 cgacttaggc aatggtttct atggccaggt gtatggcggt tatctggaaa cgatgtacgc
     4141 cggtgtcggt tccgagctgc tttatcgccc gctagatgcc agctgggcgc tgggtgtgga
     4201 cgttaactac gtgaagcaac gtgactggga caacatgatg cgcttcaccg attattccac
     4261 gccaactggt ttcgtgacgg cttactggaa cccgccgacg ctcaatggcg tactgatgaa
     4321 acttagcgtt gggcaatatc tggcaaaaga taaaggggca acgatcgacg tcgccaaacg
     4381 ctttgacagc ggcgtggcgg taggggtatg ggcggcaatc agtaacgtat ctaaagatga
     4441 ctacggcgaa ggcggcttta gtaaaggttt ttatatctcg attccgttcg acttgatgac
     4501 cattggacct aaccgcaacc gcgcggtggt ttcgtggaca ccattgacgc gtgatggtgg
     4561 acaaatgctg tcacgcaaat accagctcta tccaatgacg gcagagcgag aagtaccggt
     4621 tggacaataa cctgacgtaa ttcataacat tacattgact gataaatata aaccaatgaa
     4681 aaagaacatt tttaagtttt cggtactgac attggctgtt ctgtctttga cagcatgtac
     4741 ccttgttcct ggtcagaatt tatcgaccag taacaaggat gtcattgaac tacctgacaa
     4801 tcagtatgac ctggacaaaa tggtcaatat ctatcccgtc acgccaggat tgattgatca
     4861 gttgcgcgcc aaaccgatta tgtcgcaggc taacccggag ctggagcagc aaatcgcaaa
     4921 ctatgaatat cgtatcggga ttggtgatgt gttgatggtt acggtctggg atcacccgga
     4981 actaaccacc cccgcagggc aataccgtag cgccagtgat accggcaact gggttaacgc
     5041 cgatggtgcg attttctacc cctatattgg tcgcctgaaa gtggccggga aaacacttac
     5101 ccaggtacgc aacgagatta cagcgcgtct cgattcagtc attgaaagcc cgcaagtcga
     5161 tgtcagcgtc gcggcgttcc gatcgcaaaa agcgtacgtc accggcgaag tcagcaaatc
     5221 gggtcagcag ccgattacca atattcctct gaccatcatg gatgcgatta acgccgcggg
     5281 cggtctgacc gcagatgcag actggcgcaa cgtggtgctc acccagaatg gcgtaaagac
     5341 caaagtgaac ctctatgcgc tgatgcagcg tggcgacttg cgccagaaca aactgctgca
     5401 cccgggcgat attttattta ttccgcgcaa cgatgatctg aaagtcttcg tgatgggtga
     5461 agtgggtaaa cagagcacgc tgaaaatgga ccgcagcggt atgaccctgg cggaagcgct
     5521 gggtaacgct gagggtatga atcaggatgt cgctgatgcg acggggattt ttgttattcg
     5581 tgcaacgcaa aacaagcaga atggcaaaat cgccaatatt tatcagctga atgcgaagga
     5641 cgcatcggca atgatactcg gaacggaatt ccagcttgaa ccttacgaca ttgtttatgt
     5701 cactaccgcg ccgctggctc gttggaaccg tgtgatttct ctgctggtac cgacgatttc
     5761 cggggtgcat gacctgacag agacttcacg gtggattcaa acatggccca actaaaattt
     5821 aactcaatcc tggtggtttg taccggcaac atttgccgtt cgcccattgg cgagcgcttg
     5881 ctgcgtaaac gtctgccggg cgtaaaagtc aaatcggcag gtgttcatgg tctgataaaa
     5941 caccctgcag atgcgacagc ggcagatgtt gccgctaatc atggcgtatc tcttgaagga
     6001 catgccggac gtaagctcac tgctgagatg gcacgaaatt acgatctgat tctggcaatg
     6061 gagtcggaac atattgctca ggttaccgca attgctcccg aggtccgcgg gaagacaatg
     6121 ctgtttgggc aatggctgga acagaaagag atcccggatc cctatcgtaa aagtcaggac
     6181 gcatttgaac atgtctacgg tatgttggag cgcgccagtc aggaatgggc gaagcgcctc
     6241 agccgataat ttgagtttat aaaatacgat gacaactaaa aatatgaata cgccaccagg
     6301 cagcactcag gaaaatgaga tcgatctgct tcgtctggtc ggcgagttat gggatcaccg
     6361 taagtttatt atcagcgtga ccgcgttatt cacgctgatc gctgtcgctt actcgctgtt
     6421 aagcacacca atttatcagg cagatactct ggtccaggtt gagcaaaaac agggcaacgc
     6481 cattctcagc ggcctgagcg atatgatccc taactcatcg cccgagtctg caccggagat
     6541 ccaactgctg caatcgcgca tgattctcgg taaaaccatt gctgaactga atctgcgcga
     6601 catagttgag cagaagtatt ttccgattgt gggtcgcggc tgggcgagat taaccaaaga
     6661 aaaaccaggt gagctggcga tcagctggat gcatattcca caactgaatg gtcaggatca
     6721 gcaactgaca ctcacggttg gggaaaacgg ccactataca ctggaaggtg aagagttcac
     6781 cgtcaatggt atggtcggcc agcgtctgga aaaagatggc gttgcgctga ctatcgcgga
     6841 cattaaggcc aaaccaggaa cacagtttgt cctgagccag cgtaccgaac tggaagcgat
     6901 taacgcattg caggaaacct ttaccgttag cgaacgcagt aaagaaagcg ggatgctgga
     6961 acttaccatg actggtgatg atccccagtt gattactcgt attctgaaca gcatcgctaa
     7021 caactatttg caacagaata tcgctcgcca ggcggcgcag gattcacaaa gccttgaatt
     7081 cttacagcgc cagttacctg aagtgcgcag cgagctggac caggcggaag aaaaactcaa
     7141 cgtttatcgc cagcagcgcg attcggttga ccttaacctg gaagccaaag ccgttcttga
     7201 gcagattgtg aacgttgata atcaactcaa tgagctgacc ttccgcgagg cagagatctc
     7261 ccagctgtat aagaaagatc acccaactta tcgtgcgctg ctggaaaaac gccagacgct
     7321 ggagcaagaa cgcaaacgcc tgaataagcg ggtatcggca atgccttcca cccaacagga
     7381 agtgttgcgt ttaagtcgtg acgtagaagc gggccgtgcg gtatatctgc aattacttaa
     7441 ccgccagcag gagttgagta tttcgaaatc cagtgccatt ggtaacgtgc ggattatcga
     7501 cccggcagtc actcagccgc agccagtgaa accgaaaaaa gcgttgaatg tggtgcttgg
     7561 ttttattctt ggcctgttta tttctgtggg tgccgtgctg gcgcgtgcga tgttgcgtcg
     7621 tggtgtagaa gccccggaac aactggaaga gcacggcatc agcgtttatg ccactatccc
     7681 aatgtccgag tggctggata aacgcacccg tctgcgtaag aaaaatttat tttctaatca
     7741 gcagcgccat cgtactaaaa atatcccctt cctggcggtg gataacccgg cggattctgc
     7801 tgtggaagcc gtacgtgcgc tacgaaccag tctgcatttc gctatgatgg agacggagaa
     7861 taacattctg atgatcaccg gtgcgacgcc agacagtggt aaaacgtttg tcagttcaac
     7921 tctggcagcg gtgatcgccc agtccgatca aaaagtgtta tttattgatg ccgacttacg
     7981 ccgtggttat tcgcataacc tgtttaccgt gagtaatgaa catggcttgt cggaatatct
     8041 ggcaggtaaa gatgagctca acaaagtgat ccagcatttt ggcaaaggag gctttgatgt
     8101 gattactcgc ggtcaggtgc cacctaaccc gtctgaactg ctgatgcgcg atcggatgcg
     8161 tcaattactg gaatgggcga acgaccatta cgatctggtg attgtcgata cgccgccgat
     8221 gctggcggtg agtgatgccg cggtcgtggg gcgttctgtt ggcaccagcc tgctggttgc
     8281 gcgttttggc ttgaacaccg ccaaagaggt gagtttgtca atgcagcgtc tggaacaggc
     8341 aggcgtcaat attaaaggcg ctatcctcaa tggtgtgatt aaacgcgcca gcaccgctta
     8401 cagttacggc tataactatt acggttatag ttactccgag aaagagtaaa actgaaagtg
     8461 gccggatgcg gcgtgaacgc cttttccggt ctacgcatta gacagttctt cgtttgtcat
     8521 cagcctcaga gcattcaggt aactgaatgc tcttttttat gcattacaaa ctgcacgccg
     8581 gtatgcgtgc ttcattcacg atttgcgtaa aacctgccaa cgaacacatg ccctgcgcat
     8641 ttcgctcttc acatcctgcc agggtcagtt tcacctctcc gggcggcgta tttaatgaca
     8701 gcggcgtttt atcacgcatc tgctgtaaag tctggaagac cagcgaaacc tgaatccact
     8761 ggctgttatc gcttagccga cgccagcgtt caaacaccag ttcaccacct ggcggcgtgt
     8821 tatccggctg accgggaagc gtccagttga gctccagtgc gccgccgaga tttgccagat
     8881 tagtatcgtg tccggcgata aacagcactg aagtgggtaa tgtcacacca tacgcctgtt
     8941 tttgcggtgg atggggcgtc aacgctgtct tgatcaaatc taataacggg gtggcgcggc
     9001 tgcgggcaac ctctggcgtg cgttgtagca aataaaattg cgcgttatgc aaacttagca
     9061 aggtgttcca ctggtgtgaa tcggtgatcc ttccccaccc cggctccggc attccctgtg
     9121 cttgttgcag gagaaatatc tccgtcagca ttgatgcgag gcttaccgca ccggttaatg
     9181 agacattgtc ggcgctcacc ttgagttccg atggtaatgc ctgcgttaat gaacagcttt
     9241 cgtcctgttt ctcacgttta aggcacaagt ttgattgcgg aaaattaagc acccgttcca
     9301 gttcgcgaaa cgccgtttgc cgatgcccgg taaagtcagc aattgaccct cctgccctgc
     9361 tgaggatcgc gtcagtcacg ttcgcgttat ccagttggca aacgccagtt tttagaggat
     9421 taaataacgg atcgggactg gacgtatcag cctgggtatg tacggttatt gcacagtcag
     9481 gtgccagccc ggcggcgaag gcttcgcctg ttttacgggt acgctcgtcg acatcagcaa
     9541 taatcgcgac ctgaccagac tgcgggcagc cctttttcgc cagcaatccg tcggctacca
     9601 gacgctggcg ttggtaatgt ccgagatagg cgattagctc accaccgcgc ggtgtcagcc
     9661 aacccagttt taccggccag gttggccatg cgtctggggt gacatcctgc atcagttgcg
     9721 tggccttggt tggagcacgc acaccatgac gactgacaat caccacactt tccagcttca
     9781 gctccggctc actctgagcg aatgcagatt gcggggttaa cggaatcaga agagataaaa
     9841 atgggattaa gatcgctttc atcgatatgt ttccgctttt catatctgac attattgatt
     9901 gcctgatgcg atgctaatgc gccgcatcag gcctacgttc gttaactttt cagacgcggg
     9961 tccagccata ccaacacaag ggtggagagc gaacacatca acaaaatgcc gacgaaccaa
    10021 agtaaatacc acattgttcc tgctccttag tacaactcgt tttcgttacg gcggagagtt
    10081 tctgttgtca tgcgtcccca cattttgtag tagctccaga gagtgtagag caacacaatg
    10141 ggcaaaaata tcagcacgat taccaacata atgctcagcg tcagctgact ggaagtgctg
    10201 tcccacaacg tcaggctgga gattggactc acgcttgacg gcatgacaaa ggggaacagc
    10261 gtgatgcctg ccgtgaaaat cacgccaaat tgcatcaatg acgccattaa aaatccccaa
    10321 cccgggcgac cacgataaat cgccatcacc gtcagcaatg ggcagaagaa ccccagcagc
    10381 gggaagatcc acaaaacggg cgattcgacg aaattattca tccaggcacc aggtagcact
    10441 gccaccagtt tcattaacgg attggaagga ccgttagcat cctgggcgag cagtacaaag
    10501 ccatcaatac cgacccacag ccagtaaccc gccagcaaaa agcacagcat caccaacagt
    10561 gcagcgcgtt tggtggccag ctgtgaacgc agatgaatca caccaacagt tttcagttgt
    10621 aaccagacgc caccttgcaa aatcaccatc ccaaggctga gcaatccgca caataaaggg
    10681 aatggcgtca gcagttgcca gaagctgccg agatactcca cgcgtaattg cggtgtgaag
    10741 gcaaacggca cgccgagcaa caagttgccg aaggcgatac cgaagactac cggcggcacc
    10801 agactgccga tgaccagacc ggcgtcccac attttacgcc agcgtgcatc ggcgattttt
    10861 ccgcgataat caaaggccag cgggcggaag aacagtgagc acagcaccag gatcatcgcc
    10921 acataaaagc cggaaaacgc cgctgcatac actctgggcc aggcggcaaa taatgcccca
    10981 ccagcgagga tcaaccagac ctggttgcct tcccagtgtg caccaacgct gtttatcact
    11041 atccggcgtt catcatcatt acgcgccacc agcggcagca gacagccgat ccccatgtca
    11101 aatccgtcgg agatcataaa gaccaccagg atcacgccaa tcagcagcca ccagatgaag
    11161 cgcaatgttt cataatcaaa catgattttc ccctttaccc ctgttgctgc gtcggttgtt
    11221 cactctgcat cgcgctcggc cccagacggg catatttctg catcaggtag acttcggcga
    11281 ttaagaacag ggtgtaaagc cctacgatca tgatcagtga gaaagccagt tgtcctgtgg
    11341 ttaaagcgga gtgcgcggag tatgtcggta agatgtcctg tatcgcccac ggctgacgac
    11401 caaactcggt cataaaccac ccggcttcaa tcgcaatcca cggcaacggc aaactccaga
    11461 gcgccatttt cagcacccag cgatgctggt cgattttgcc acgcagcgtc tggacaagcg
    11521 caatcagcat caccagtagc ggcagggaac cacagcccac catgatgcgg aaactccaga
    11581 ataccggcgc aacctgaggt atcgcgccac gcatcgccgc ctggtactgt gcggctgtga
    11641 catgattcat atccggcgca tagcgggaga gcaacatgcc gtagcccagg tcaccttcca
    11701 gttcccggaa tgcctgcaac acatgcggct cacgattgcc ttgcgatatt tcctgcatta
    11761 acagccaggc catacgtccg cgttgcaagc gtgggtaggt ttcagccatc aaatttttca
    11821 gacccggcac ggttttatct aatgagtgag tggcgaggat ccctaacagc gcgggaattt
    11881 tgatggcaaa ggcgttacgc tcttgatcct gttccggcca ggcaaccaca tggaacggtg
    11941 caggagcagg ttccgtttgc cactcccctt ccatcgccgc cagttttacc ggttgtactt
    12001 gcgcgacttc atacgcagaa ctgtctccga gttgtagcgt gccgataatc gccagagtac
    12061 cgaagacgga accgatggca aacgagcgta atgcaacatc gcgctcccgt ccgcgcagta
    12121 aataccaggc gctgatcgcc ataataaaca tggccccggt cacgtagccc gccattacgg
    12181 tgtgcacaaa tttcacctgg ctgaccgggt taaagaccag ctcgctgaag ctggtcatct
    12241 ccatacgtag ggtgtcgata tcaaaatgcg caccggtcgg gtattgcatc caaccgttgg
    12301 cattcaatat ccacaatgca gagagatttg aaccgaacgc caccagccac gtcaccagca
    12361 ggtgctggta tttattcagc cgctgccagc cgaagaagaa aagcccgaca aaggtggatt
    12421 cgaggaagaa ggccattaat gcttccatcg ccagcggtgc gccaaaaata tcgcccacat
    12481 agttggaata gaatgaccag ttagtaccaa actgaaactc catggtcagg ccggtagcca
    12541 cgccaagagc aaagttgata ccgaagagct taccccagaa gcgcgtcata tcgcggtaga
    12601 ttgttttgcc ggtgaccacg taaatggttt ccatgatagc cagcaaaaaa atcagcccca
    12661 gggtaagggg tacaaataaa aagtgataca gcgcggtcag agcaaactgc cagcgcgata
    12721 aatcaatgac atcccacatg cgcactcctg taggcgaaga gagaaaaata acccgataat
    12781 gttagatctc cctctttatc ctgaaactct acgaccagag gtattcgccg attgcgatat
    12841 tcataagact gaaagcgata cttaccgtct ttttacgtcg gtgcagcttc cgccagccac
    12901 tgacatacct cgctaagccg ctgcgcagag tcgaccaaat cttctggcgc tgccagcacc
    12961 acttccggta ttgggcagat ttcataactt tccagtaaag gaccttttaa ggtgtccgta
    13021 cagcgtaaat gccagacatg gcgtaacccc gtggcgttga tatagctctc gccatagcca
    13081 atggtacgaa tctgaatatt tcccggccca cagagacgtg agagaaaaag ccgatcagcc
    13141 tcgctgatgg gtaattgcgt cagattaatg ctgtgcggct gcgcgtcggg gttacgtaca
    13201 tgcgccagta actcatgcgc caacggtagg ccattcatca ggccatcaat gggcggcggt
    13261 aacagcgaat ctgtcggcaa gagattttgc gtggccgcct gccacaacgc cagcggcgcg
    13321 cagccggcct ccagtttgtc ctccagcaac ttttcgccgc gacgtttgcg cacccgccat
    13381 aatccgcaga agatcgcctc ctgtatttca ctttcgctgt cgtcagcctg ctgaatgcgc
    13441 actgacactt ccccttcccc gagaagcgta ttgataaagt gacgatcgtc ggcgttgaga
    13501 tttaacagat cgactgccag cacctcgccc aacggcggct ggcgttgact tagttgatgt
    13561 tgtaactcgt ttaacagcgc aatcacctgc gggctgtgag cacattgctc cagggccgcc
    13621 atactcggtt catcattcac ctgacagctg atcggcagtg gattcatgct gaaactgtca
    13681 tcgttcggtt gcgttcctgg ccccagcaga tggaaaaaag tttcgctcat gaggcacgct
    13741 cctgttgtgg ttcgacaagc ccacgcatca ggtttatcag ttccgctcac gggtgaatac
    13801 cattcagcac accgcgatag tttccgccgg taaacactaa tgtggcggga aagcgaaaga
    13861 cgccaaagcg atctccgatg gcttcgctct gttcaaggtc agcaatcgcc acctgccatg
    13921 tgtagccggg aaactcgcgc agtaattcgc caatcattac cggattatcg ctgacctctg
    13981 gcgtgcgttt cgggtcactg cttaataaca ccacgccgtc tggcgcttgc gtaagccagt
    14041 cgtcaagacg gaattcacta accggcgtcc agccgcgcgc cagcattcgt tgccacaacg
    14101 catcaaatgg cgtgtcgttg ctcatccctg ctcctccagt gtcatcctgt actggcgcaa
    14161 gcgaacgcct tcgtaatttt ccatcgacag acagtcataa ttgagacagc gcgattcatt
    14221 agccggttgc ggcacaattc cccatgcagc cagctgcgcc agcgccgcct gttccgctgc
    14281 gggcagttgc tcccgtgcca gttcgctcag gctgccgccg tagtcgtcga gcattgcggg
    14341 ttgcagaccg acgagggcaa tatgtgctgg cagatgtccg cggatatctg ccagcgccag
    14401 cacttcggag aaactgttct ggtgcaggct cattttcttc gcgctgagat aagccggaat
    14461 acgttctccg gcataggttc gcagcgttcc aggttccagc ccgtagtcaa tggcatcgag
    14521 aatcaacaga tggctggcgc tttcgacata ccccagcaag ttcagtccct gagtaccgcc
    14581 atcgacaatc tccacatact cgggccagtg gtaatgggca tacaaccgtt ccgccacccg
    14641 cacgccgaaa ccttcatcgg cccacagcag gttgcccagc cccattacca caacgcgttg
    14701 ctcgctcatg aacgctcctt gttacttatt ttgccaaatt tgtggctacg gtagccgttg
    14761 accatggtgg agatcaccgt gtcgtcggac atgatgtctt cacgcagcgc catatagaca
    14821 tgaccgatca caaacgcgcc aatcagccac atccccagcc gatgccagct gtgaatgtcc
    14881 attgagttgc ctcccgtcca gtagaaaaat tccaccacat aacggaacgg cgcaaaaata
    14941 gcgtactggc tgtgttcgct gtacagcgca aagccggtga tgatcataaa gaccgacatc
    15001 aggaaatagc cgaacatcgc cgcctgggcg atggggttat ggcctatatc ggcactcgga
    15061 cgttttgcca gaacagatac cagcggattt cataccacac gccctgccac cagcttttac
    15121 gccataccgg cacgataaac agctcgcggg agtatcgatt gccaacaaaa gcccagtaga
    15181 tccgcatcag caagaccacg gtaaaaacca tcccggcgct gaagtgaatt aacctgatgt
    15241 agcccatata gaacagatac gtcgcctcgc cgctgacgga aggtagcggc ttgccgataa
    15301 agtatccggt gaccatcaac accgccatgc ataacaccgt cagccagtgc cagatgcgga
    15361 ctggcgcttc aaagacatag tggctgacaa cgttgtcgct tttctgttgc atgatgattc
    15421 tccttcgctg ttaacgcacc tgcacggaga tcagctcgct accgtcgtcg cccagcacgt
    15481 gtgttgaaca ggcgaggcac gggtcaaagc tgtgcagagt acgcaggatc tccagcggtt
    15541 gctcggggat cgccattttg gtgttcatca gcgccgcttc ataagcgcca atctgccctt
    15601 tgggatcgcg cgggctggtg ttccaggtgg tcggcaccac gcactggtag agatcaatct
    15661 tgccatcgcg aatggcggcc cagtggccta acgccccgcg cggcgcttcg gtaaagccga
    15721 cgccacggca ctctgtcggc caggttgcag gttcccattt ttccgtggag gcggtggcaa
    15781 gattgccgtt tttcaggtta gtcatcagct tgttgaagaa atattgcaac ttacctgcgg
    15841 cccactgtgc ttcgtgcgcg cggcacaaaa tgcggcccaa cgttgactgg ataccggaaa
    15901 gcggcaggtt cagcgccgac atcatgcgat cgaccgactc aacggtcgca gcatcgcctt
    15961 tgtgataagc gattaacgtg cgtgccagcg gccccacttc catcgcgtta ccgcgccagc
    16021 gcggcgcttt gatccacgag tagcgttcct gttcattcag ctgctgaatg ttggtatcgc
    16081 tgcctttgac atcgccgggg ttgtaccacg ggtcggtaat gccatcgaat ggatgacgcc
    16141 cgacctgatc gttgggataa cgataccagg cgtggtcgac aaactcctgc acctgctgcg
    16201 gatcaaccaa atccactggc agcacattgt tgaagtcgcc gttaatcacc gcgccgccag
    16261 gcatcagcag acttttctca ccaaagtcgt tggcaatatc cgggaacgca ccgtagctga
    16321 ggacacattt atcagaaaga cccgtgccga tttcgctcca cggtttgttg aactgaccga
    16381 tggctaaggc gtcggggatc atcacgttgt taatgaagtc cgccgtgcgg gtgataatcg
    16441 actgcaccag gttcaggcgt tccatattga ccgccccgac cgcgccgctt tcgtcaatgt
    16501 taatggcgca aggcatcccg ccgacaatcc agtttggatg cgggttttta ccgccaaaga
    16561 ccgcgtggat tttgacaatt tcacgctgga aatcgagagc ttcgagatag tgggcaaagc
    16621 ccatcaggtt agcttctggc ggcagtttgt actgcgggtg cccccagtag ccattgcgga
    16681 agatccccaa ctgcccgcct tcaacaaatt ttttcaggcg gttttgtacg tcgaagaaat
    16741 agccagggga tgatttcggc caagaggaga gactttgcgc cagttcggag gttttccgcg
    16801 ggtcggcttt cagcgcatct aacacatcga tccagtccat cccggcaagc tgatagaagt
    16861 gcaccagatg atcgtggcac cagagcgttg ccagcataat gttgcggatg atattggcgt
    16921 tgtccggcac tttaataccg atagcatctt cgatggcgta aaccgaagcc agggcgtgta
    16981 cgccagtaca gacgccgcag atacgttcaa cgaacgccca cgcatcgcgc gggtcgcgcc
    17041 cttgtaggat gatctccagc ccgcgaaaca tggtgccgca ggagacggca ttggtgatca
    17101 cattctgatc gttaatattc acttcgcagc gcatgtggcc ttcgatgcgc gtaatcgggt
    17161 cgaccaccag gcggcgtccg gcattattga tggtgtatcc ctgagtttcg tactgagtgc
    17221 tcatgcctgt ttatcctcat tgcctggctg atgttcggtt tctgtaggtt gctggttatg
    17281 acgtctgcgc tggtcaacgg cgctggcgac tgcgtgcaca ccaacagccg ctgccaccac
    17341 accaagcgcg gttaaaccga cggtatcggc ggtggaatga gtacccattt gcggaatatc
    17401 gaccacgcgg ctgtagaacg aaccgcaatc ccagaaacca ttttccgcac agcccaggca
    17461 gccgtgacca gactggattg ggaaagaaac gccatcattc cagcgtgtgg aggaacaggc
    17521 gttataggtg gtaggccctt tgcagcccat tttgtacagg cagtaacctt tgcgggcagc
    17581 gtcatcatcc caactctgga cgaactctcc ggcgtcgaag tgggcgcggc gatagcattt
    17641 atcgtggatt cgctgaccat agaacatcag cggacggccc attctgtcga catctggcaa
    17701 gcgatcaaag gtcaccatgt aagtaatgat ggcgctcatc acatccggga tcggcgggca
    17761 gccaggtact ttgataatgg gtttgtcggt gatgactttg tcgataggcg ttgcctgcgt
    17821 cggattgggc cacgcggcct gcacgcagcc ccaggacgcg caggttcccc aggcgataat
    17881 cgcgctggct ccggcagcgg cacgtttgag tttctcaata aacggtcgac cgctgctgat
    17941 acagaacatc ccctgctcgc ccagcggcgg attaccttct actgcgagga tatatttgcc
    18001 attgtattgc gtgatgatgt cttcgaagac ttcttccgcc tgggttccgg cggcagccat
    18061 caaagtatcg tcataatcga gggaaatcag ggaaaggatg acgtccttcg ccagtgggtg
    18121 agcggagcgg ataaaagatt cggtacagca ggtgcattcc agaccgtgga tccataccac
    18181 cggaatgcgg ggtttgttct ccagcgccca ggcaatcttt ggtgccattc ccgcgcctaa
    18241 tcccagcgac gtggcagcca gactacaata tttgagaaag ctgcgccggg taacgccctg
    18301 acgccgcatg gcctggtaaa atgtttcctc gttattcata tcgcacgtct cttctccttg
    18361 cgacaccggc aggacaccgg gcgcttatgg tcggcaagcg acgtccctgt agcaaggcaa
    18421 accatcgctt cacgtgagat gctgaaaacg aaagctcatc cttctgcact ggcgcacgtc
    18481 gccagaaagt attgttaata aagcgtagtg aaa
//
LOCUS       DAJTZL010000078        18322 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.078, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000078 DAJTZL010000000
VERSION     DAJTZL010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18322
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.078"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..1106)
                     /locus_tag="OO544_002296"
     CDS             complement(<1..1106)
                     /locus_tag="OO544_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCR6767880.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALVGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVAAERQHVLAFALAGGVLLLMAVVVALSFG"
     gene            complement(1103..1993)
                     /gene="fhuD"
                     /locus_tag="OO544_002297"
     CDS             complement(1103..1993)
                     /gene="fhuD"
                     /locus_tag="OO544_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HCR6767881.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSSEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRAGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            complement(1993..2790)
                     /gene="fhuC"
                     /locus_tag="OO544_002298"
     CDS             complement(1993..2790)
                     /gene="fhuC"
                     /locus_tag="OO544_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HCR6767882.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRSGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            complement(2841..5030)
                     /gene="fhuA"
                     /locus_tag="OO544_002299"
     CDS             complement(2841..5030)
                     /gene="fhuA"
                     /locus_tag="OO544_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HCR6767883.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAAKHSATATKTDTPIEKTPQSISVVTNEEMQMRQFQSVKEA
                     LGYTPGVTVSSRGASNTYDFVIIRGFSSVGLNQNNYLDGLKLQGNFYNDAVIDPYMLE
                     RVELMRGPTSVLYGKSNPGGIISMVSKRPTTEPLKEIQFKMGTDNLFQTGFDFSDALD
                     DNGEFSYRLTGLARSTNEQQKNSESQRYTIAPSFSWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGASNNTYSRNQKMVGYSFEHGFNDTFTVRQNLR
                     FSEMKTSQKSVYGTGIAGDGHTLNRGTVVDNERLQNFSVDTQLESKFATGDVEHTLLT
                     GVDFMRMRNDINASFGSAPSIDLYNKYHPEYFAFGSAEPYQMNESKQTGIYVQDQAEW
                     NKWVFTLGGRYDWSKQATTVRENSYTPTEGYIERNDHQFTWRGGVNYLFDNGISPYFS
                     YSQSFEPSAFDLWSNPRISYKPSKGEQYEAGVKYVPNDMPVVVTGAVYQLTKTNNLTA
                     DPTNPLAQVPAGEIRARGVELEAKAALNANINLTASYTYTDAEYTKDTNLKGKTPEQV
                     PEHMASLWGDYTFNEGPLSGLTLGTGGRFIGSSYGDPANTFKVGSTAVMDAVVKYDLA
                     RFGMAGSSLAVNVNNLLDREYVASCFQTYGCFWGAERQVVATATFRF"
     gene            complement(5250..7829)
                     /gene="mrcB"
                     /locus_tag="OO544_002300"
     CDS             complement(5250..7829)
                     /gene="mrcB"
                     /locus_tag="OO544_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HCR6767884.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDGELYE
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMF
                     GSN"
     gene            complement(8025..10454)
                     /gene="hrpB"
                     /locus_tag="OO544_002301"
     CDS             complement(8025..10454)
                     /gene="hrpB"
                     /locus_tag="OO544_002301"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HCR6767885.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERHYLPLPAHQRFDEAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFEPRTGLTRLITQRVSQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPVVNLMAAKRL
                     LQMLGALDGERLSAQGQKMAALGNDPRLAAMLVSAKNDDEAATAAKIAAILEEPPLMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIAHRRGQDG
                     RYQLANGMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLAALETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGGVPLVLELLSPAQRPLQITRDLSAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            10528..11058
                     /gene="thpR"
                     /locus_tag="OO544_002302"
     CDS             10528..11058
                     /gene="thpR"
                     /locus_tag="OO544_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HCR6767886.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            11073..11777
                     /gene="sfsA"
                     /locus_tag="OO544_002303"
     CDS             11073..11777
                     /gene="sfsA"
                     /locus_tag="OO544_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HCR6767887.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATRGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMNVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLSVTL"
     gene            11955..12410
                     /gene="dksA"
                     /locus_tag="OO544_002304"
     CDS             11955..12410
                     /gene="dksA"
                     /locus_tag="OO544_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HCR6767888.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            12447..13373
                     /gene="gluQRS"
                     /locus_tag="OO544_002305"
     CDS             12447..13373
                     /gene="gluQRS"
                     /locus_tag="OO544_002305"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HCR6767889.1"
                     /translation="MLPPYFLFKEMTDTQYIGRFAPSPSGELHFGSLIAALGSYLQAR
                     ARQGRWLVRIEDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWL
                     HEQGLSYYCTCTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDQLRGII
                     HADEKLAREDFIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQISLYQLF
                     GWKVPDYIHLPLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFS
                     VEQILQSAVKNWTLTAVPESAIVNSTFSNASC"
     gene            13412..14830
                     /gene="pcnB"
                     /locus_tag="OO544_002306"
     CDS             13412..14830
                     /gene="pcnB"
                     /locus_tag="OO544_002306"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HCR6767890.1"
                     /translation="MTLPRCTIFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQH
                     AISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKL
                     FRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGS
                     IEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLR
                     AVRFAAKLGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEY
                     HLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLL
                     ETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQG
                     KRAWKLLEHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDE
                     EPSPRRRTRRPRKRAPRREGTA"
     gene            14827..15306
                     /gene="folK"
                     /locus_tag="OO544_002307"
     CDS             14827..15306
                     /gene="folK"
                     /locus_tag="OO544_002307"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HCR6767891.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESHILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSLAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAFDELSKW"
     gene            15676..16260
                     /locus_tag="OO544_002308"
     CDS             15676..16260
                     /locus_tag="OO544_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6767892.1"
                     /translation="MSKKLGFALSGLMLAMVAGTASADMDGGQLNISGLVVDNTCETR
                     VDGGNKDGLILLQTATVGEIDAGVLNDTVGAKAKPFSITVDCSKANPNPGSTAKMTFG
                     SVFFGNSKGTLNNDMSINNPSDGVNIALHNIDGSTIKQVQINNPGDVYTKALDATTKS
                     AVYDFKASYVRAVADQTATAGYVKTNTAYTITYQ"
     gene            16365..17105
                     /locus_tag="OO544_002309"
     CDS             16365..17105
                     /locus_tag="OO544_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HCR6767893.1"
                     /translation="MFFNTKHTTALCFVTCMAFSSSSIADIVISGTRVIYKSDQKSVN
                     VRLENKGNNPLLVQSWLDTGDNNAEPGSITVPFTATPPVSRIDAKRGQTIKLMYTAST
                     SLPKDRESVFWFNVLEVPPKPDAEKVANQSLLQLAFRTRIKLFYRPDGLKGNPSEAPL
                     ALKWFWSGSEGKASLRVTNPTPYYVSFSSGDLEASGKRYPIDVKMIAPFSDEVMKVTG
                     LNGKASSAKVHFYAINDFGGAIEGNASL"
     gene            17140..18322
                     /gene="htrE"
                     /locus_tag="OO544_002310"
                     /pseudo
     CDS             17140..18322
                     /gene="htrE"
                     /locus_tag="OO544_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Yad fimbria usher protein HtrE"
BASE COUNT         4529 a         4931 c         4496 g         4366 t
ORIGIN      
        1 ccaaacgaca gcgccaccac cacagccatc aacagcagca cgccgcccgc gagggcaaac
       61 gccagcacat gttggcgttc agccgcgaca cgatcgttga ccttcatatc cggcgcgcta
      121 atgctgcgta aacgcggcaa cagccacagt agcagcggcg caccgatcaa cgcagtgacc
      181 gaaccggtgg acacttccat ccacacgcga gtcagccaga ggatgatttg atcggaaagc
      241 cagaggatca gcgccccaat cagcgacgcc agcatcaacc gtggcaacaa acgccgcgcc
      301 cccagcattt tcgccagcag cggcgcgaac aaaccgataa agccgataat ccccaccgca
      361 ttcaccagca gcgcactgat gactatcgcc agcgacagcg ccgccagacg cgcgagcgac
      421 aaggccagcc cgagattgcg cgccacgcca tcatcaagcc ccatcagggt taacgggcga
      481 agtagcaata acgtcaacat cacgccgccc agcaactgcg gccataaacg ctcaacgccg
      541 ccccagtcgg tttgcgtcag cgttccggtg ctccacagga acatgctttg cagctggtcg
      601 tgatggaaga taaccagtag ctgattaatt gccccgcaat aaaggctcac caccagcccc
      661 gcgagaataa gcgtaaccgg cgaaagccgt ttcccccacg cgacgccaaa gacgattaag
      721 ccaacgacac aagcgcccac cagtgcggca aactggctcg ccatagcacc tgggatcgcc
      781 caaagtgtgg tgacggtaat tcccagttgc gcgcctgtag caacgccaag cgtcgtcggc
      841 tccgccagcg ggttacgcag cacttgctga aacagcacgc ccaccagccc caggcccgcg
      901 cccaccagca gcgaaatcgc cagacgcggc aacaagctgt agtgaaaaat catctgctcg
      961 atgacatcga tgtctggcga ccaggcagcc tgcgcccact ggctacgcgg cagcgcctgc
     1021 gagaagttca tccaggtgag cgccgtagcg acaatcacta acagcgccag caataacgcc
     1081 ggaaaaagcg caattcgttt actcacgctt tacctccgat ggcgttatcc agaacgcgca
     1141 caaagtgcat tgccgagagt gtcgcaccat aaaaccagac tgcaggtacg cgctgaaagc
     1201 gtccggcgcg gacaaacggc atggcctgcc acagcggggt tgccattagc gcatccatgt
     1261 ctttgctgtt gtcgtgatca aaacagagca catcaacgtc tttatacgct gccagacgat
     1321 cgatactgac ggcggtactg ccccagaagt tggtttcccc ttgccaggca tttgggatgc
     1381 cgtactcatc gagaatttcc tggaacaagc tgtttggacc gaagaccagc atatggcgcg
     1441 gatcgataag cgtcgtcagc agtaacgggc gtgcaccacg cttcacaaag cggggtttca
     1501 tgctgcggat aaagtcttcg tattgcgcta aatgcgtttc cgctgcgctt tgcaggttaa
     1561 gtaaatctgc catttccgtc agcgatttac gcgccatcgc caacggctgt ttgccgtcac
     1621 tgaagttaaa tccgcgaccc ggcgcaatac gtgccagcat ttctgatgaa gggccatatc
     1681 ctgccgacca gaccataaac gacggtttca tttcggtcag cagttcaagg ttaggttctg
     1741 tacgcaaacc gacgtcaatc actgagtccg gcaatggtgg ttcgctgacc cacaggcgat
     1801 agttgatggt atccgccacg ccgtaaggca cgatgccgag cgccagcagt aattccaccg
     1861 gcaaccactc cagcgccaca atacgattgg ggtcaatagc cgccgcgtgg gcggtattca
     1921 tctgccataa caacggagaa agcgccatcg ccgttaacag tcgacggcgc gaaataagag
     1981 gtaagccgct catcaataaa caaaactcac aggtgcagca cccgccggat gcggcaaaat
     2041 acccatcggg atgccataaa tcatttcgag ggtttcgccg cgcataattt ccgcaggcgt
     2101 tccctgagca atcatttcac cactgcgcag ggcgaccaga taatcacagt agcgtgccgc
     2161 catattgata tcgtgcaaca cggcaatgac cgtcaggcca cgttcctgac ttaaacggtg
     2221 caccagcgcc agcacatcaa cctggtgggc gatatccagc gccgaagtcg gttcgtcgag
     2281 caacagacaa cggctatcct gcgccaccag catggcgatc cacgcccgct gacgttcgcc
     2341 gccagagaga ctatcgacca gccgatgcgc cagcggtttt aagccaacca gcgagatagc
     2401 ttcctcgacc ttttcgcgat ctgccgcacc aaagcgcccc agcgcgccat gccacgggta
     2461 acgaccaatc gccaccagtt cacgcacggt catcccttct gccggaggaa gctgctgcgg
     2521 caaataagcc actttgcggg caaacgcttt gctgctccag ctttccagcg gctgggcatc
     2581 aagaagaatc tccccttctg acggaggctg atgacgacca agcattttga gcagagtgga
     2641 tttaccagaa ccgttgtgac caatcagacc ggtcactttc ccggcaggaa aggttaacga
     2701 cagcggatgc aaaagcgtgc gcccgggcac acgaaaggag atattacgca gtgcaaaagt
     2761 ggtatcggaa tgattcgtgt attcctgcat aacagccaac ttgtgaaatg ggcacggaaa
     2821 tccgtgcccc aaaagagaaa ttagaaacgg aaggttgcgg ttgcaacgac ctgacgttct
     2881 gcgccccaga agcagccata agtctggaag caactggcaa catactcacg atcgagcaaa
     2941 ttgttgacgt tcacagcaag gctggatccc gccataccaa agcgtgccag atcatatttt
     3001 acaacagcat ccattacagc tgtgctaccc actttaaaag tgtttgccgg atcaccatag
     3061 ctggaaccaa taaaacgacc acctgttccc aatgttaaac cagaaagcgg cccttcattg
     3121 aaggtataat ccccccagag agatgccata tgctccggta cttgttctgg agttttacct
     3181 ttgagattgg tgtctttggt gtattccgca tcggtgtagg tataagaagc cgtcaagtta
     3241 atattggcat ttaacgccgc ttttgcttca agttccacac cacgagcgcg aatctcacct
     3301 gctgggactt gcgctaatgg gtttgttggg tctgctgtca ggttatttgt tttcgtcaat
     3361 tgatagactg cgcccgtaac aacgaccggc atatcattcg gaacatattt tacgccagct
     3421 tcatactgtt cacctttcga tggcttatag gaaatgcgcg ggttgctcca cagatcgaaa
     3481 gcactcggtt caaaggactg gctatagcta aagtaaggtg aaataccatt atcgaataag
     3541 taatttacac caccgcgcca ggtgaactga tgatcattgc gctcaatata accttcagtc
     3601 ggcgtataag agttttcacg aacagtggtc gcttgcttag accaatcgta gcgtcccccc
     3661 agagtgaata cccatttatt ccattccgcc tgatcctgaa cataaatacc tgtttgtttg
     3721 ctttcattca tctggtatgg ctctgcgcta ccaaatgcaa agtattcagg gtgatatttg
     3781 ttataaagat cgatggatgg tgcggatcca aagctggcat tgatatcatt acgcatccgc
     3841 atgaagtcta cccctgtcag caaagtatgc tcgacgtcac ctgtagcaaa cttactttca
     3901 agttgggtat caacgctaaa gttttgtaga cgctcattat ccaccactgt cccgcggttt
     3961 agggtatgac catcaccagc aatccctgtg ccataaacac ttttctgtga ggttttcatt
     4021 tcactgaaac gcagattctg acgcacggtg aaggtgtcat taaaaccatg ttcaaaacta
     4081 tatcccacca ttttctggtt acgggagtat gtattattcg atgcaccttc attgaagtca
     4141 gtcggtagac gcttaccatt aggcaatggc tcaacggtcc cctctttcgg caaccaaccg
     4201 taataacccg tttctggttc attctggaaa taggacagga aagtaaaatt ggttttgtcg
     4261 tctggacgcc atgagaatga tggagcaatg gtataacgct gagattcaga gtttttctgc
     4321 tgttcgtttg ttgagcgagc gaggcccgtc aaacggtaag agaactcacc gttatcatcc
     4381 agtgcatcac tgaaatcgaa tccggtctga aacagattat ccgtccccat tttaaactga
     4441 atttctttca ggggttcagt ggtcggacgc ttactcacca tagaaataat cccaccagga
     4501 ttactttttc catagagaac agacgtcggc ccacgcatca gttcaaccct ctcgagcatg
     4561 taaggatcaa tcacagcatc gttataaaag ttgccctgaa gttttagtcc atcaaggtaa
     4621 ttattttgat tcagaccaac agatgagaaa ccacgaatga taacaaaatc atatgtatta
     4681 gaagcaccac gactgctaac cgtaacgcca ggtgtataac ctaatgcttc ttttacagac
     4741 tgaaattgac gcatctgcat ctcttcattt gtcacaaccg aaatagactg cggcgttttt
     4801 tcaattggtg tatccgtttt agtcgcagta gcagagtgct tcgccgcaat agttgcagca
     4861 ggcccccatg cgctttcttg cggcgcaggt gcagcggtaa cggtgatagt gtcttctttc
     4921 ggttcaaccg ctgcctgtgc ataaacagac atgccgctaa ccgctgtggc tactacaact
     4981 gcgattttac gcagtgagtg ttttggctga gcagttttgg aacgcgccat tggtatatct
     5041 ctgatgtaaa gtgaatgata acgtaaacga gaatgattat tataaagata agataatatt
     5101 cgaaacgctg gcgggttcgc aagcggtacg caatctgagc gttcctgaag ggttaataaa
     5161 caaccagatg aaaagaaagg gttaatactt taggtgggat gttattttac cggatggcaa
     5221 ttcgccatcc ggtatttcac gcttagatgt taattactac caaacatatc cttgatccaa
     5281 ccggctacac cgtcgctgtc tttctgctct tgctgagcag gttgctgttg cggctgttgc
     5341 tgcggctgag aagactgatc aaacggattg cctgacggct gttgctgcgg ctgagaagac
     5401 tgatcaaacg gattgcctga cggctgctgc tgcatctcgc tctgctggca cagcgattgc
     5461 ggatcgctgg tccagaccgg caagacacgc atgccaccgc tgcaaacaaa gttgccgtcg
     5521 tagtccacgc ccatatctgc aatatcttct ggcggaacaa gattcagcgg cgttggcgtc
     5581 tggttagcca gataacgctg ataaatagac attgccccgc tggcaccata cagtttggtc
     5641 ggctggttgt tatcacggcc tacccaggtg atggtcaccg tgctgccgtc aatgcccgca
     5701 aaccaggtat ctacgttatt gttggtagtc cctgttttcc ctgccagatg caggttcggg
     5761 tatttcgccc caagttgacg acccgtacca cgttgtacca cctgctgcat ggtccatagt
     5821 gtcagatacg ccgcctgcgc cggaacagcg cgttctgcct gcgggaagct ctgatacagc
     5881 accttgccat cttccgcgat taccgaacgc agtgcagaaa gcggtgcacg gttaccgccg
     5941 ctggcgatgg tctggaatgc ctgcgccact tcgattggcg ttaagttcaa cgcccccagc
     6001 agcatcgccg gaaccggatg cagctgatct ttcggcacgc ccagtttgat ccaggtctcc
     6061 gtaactgcag gcagccccag cgccatcccc agatttaccg tcggcacgtt catcgaacgg
     6121 gtcaacgcat ccaccagcat cactctgccg ctttcgctat aacgacggtc atcgttctgc
     6181 ggtgaccaga cctggccatt cggctgacgc agcgcgattg gcgcatcggc aatccacgtg
     6241 ttcaggcgat agattttcgg ctggcttaaa gccgtcagat aagtcgctgg ttttgcaagg
     6301 gaaccaatcg aacgacgcgc ctgcatcgca cggttgtagc ccgcaaactg cggctcagaa
     6361 cctcccacca tcgcacgaac ttcaccacta aagcggtcga cgaccacaat tgcagtttca
     6421 agatcgctca acttacgctg tttcttcagt gccggaatgc cttccacggc ggctttttct
     6481 gccgcgtcct gggccaccga gtcaaaggta gtgaagatct tcacgccgga gagatctttt
     6541 accttatcgc ctaatttcgc ctgcagctcc tgacgcacca gttgcataaa ggctggctgt
     6601 ggagagatta ccccaccgcg cggctgtacg cccagaggac gcgcgctgag catttcatac
     6661 agttcaccat cgataatctg ctgttgttgt agcaaacgca gcaccagatt acgtcgctcc
     6721 agcgccagtt tcgggttacg ccacgggttg tagatggacg cccctttcac cataccgact
     6781 aacagcgcct gctggtcgag gctgagctct tctaccgggc gaccaaagta atacaagctc
     6841 gcaagcggga agccgcggat ttcgttgtcg ccgctctgac cgagatacac ctcgttcata
     6901 tacagctcaa gaatacggtc tttgctgtaa cgcgcgtcca tgatcagcgc catgtaagct
     6961 tcgttcgctt tacgccagta agaacgctcg ctggagagga acaggttttt caccagctgt
     7021 tgcgtcagcg tactcgctcc ctgcaccgtg cgtccggcgg tcaggtttgc cagcaccgca
     7081 cgtccgatgg agtagagact gatcccatca tgctcgtaga aatgacggtc ttctgtcgcc
     7141 agcaaagtat ccaccagcaa gtccgggaaa ccactgcgcg gcacaaacag acgctgctca
     7201 ccgtttggcg aagagatcat ggtgatcagg cgcggatcaa gacggaagaa accgaactga
     7261 cggttgttct ccatattgac gatcgtcgcc agatgatcgc catcaaaggt cagacgcgca
     7321 cgcacctgcc cttctttgct gtccgggaaa tcaaacggac ggcggatcat ctcaatgctg
     7381 ttggcctgca cggtaaattc gccaggacgg gtcattttcg acacctgacg atactgtgtt
     7441 gcctccagca gcttcaccat ttcgttcttg ctgatggtca tatctggttc aaggttgacc
     7501 attcggccat aaactgccgc gggcagttgc cagaccttgc catcaatacg gctacgaatt
     7561 ttttgatcga gataaacgcc gtaaatggcg atcagcacgg caaaaacgat agccagtttt
     7621 aacagcagcc atagccagcc gcgtttgcca cgaggcttac gccctttgcc tttgccctta
     7681 cctttgcgcg gcatcggttc ttcatcctca tagtcatcat aatcgtcgta atcatcgtca
     7741 tcttcgtaac gacgacggct taccttttgt ttgaccggac gcgtcggttt ccctttgcgt
     7801 ccaattggct cgcggtcatt cccggccatg ctttttctcc gcaatattca ggcgcaaaag
     7861 cccgattttc tgttcttctg ttacaagaca gaagaagata tctctcaaaa tttgtgctcg
     7921 atcggtcccc tctcccttga gggagagggt tagggtgagg gtgaacgtgc gcgctgatgc
     7981 cctcaccccg gccctctccc acagggagag ggagaaaacc aaccttacga atactttttc
     8041 gtccgtcgcg tcggtgccgt gtttgccggg tcgtccggcc agacgtgttt gggataacgc
     8101 cctttcatct ctttttgcac ctcacggtac gctcctttcc agaaggcgct caaatctcgt
     8161 gtgatttgta gtggcctttg ggcgggtgaa agcaactcca gcaccagcgg cacgcccccc
     8221 tgggcgatag tcggattagt ggcctcacca aacatctcct gcattctcac cgccagtgcg
     8281 ggcgggttat cttcatgata acgaatggcg atccggcttc ccgtcggcac agtgtaatgc
     8341 gcaggcaatt cactatccag acgttgctgc attccccaat caagtaatcc gcgtagtgcc
     8401 tgataaatgt ccagtgattt caggccgcgt agtgaatgta cgcctgtcat atgtggcagc
     8461 agccacgttt ccagcgctgc caataaactt tcatcatcaa ccgctggcca gtcatattcc
     8521 ggcaaccact ttgcggcgca taacaaacgc aagcgtagct gttccgcttc cgccgtccag
     8581 ttgagcacgc ttaaaccttt atcacggatg ccattaagca tcgcctgatg caactcgtct
     8641 tctgacggtt tcgccagcgg ctgcactttc accgtcaact gaccgatttg tagccgacgc
     8701 caggctttca gcgtaccttg cgcgtcatcc cattccacgg tgtcagattg ctgcaccagc
     8761 tgcggacagc gttgtactaa ttcatcaata tcgaccggta gcgccagtaa aatccgcgca
     8821 tccggcgagg cgctgccctg caataataac ggtgcgatca accattcgtg gcggcttagc
     8881 gcgtcgtcgg catcgagcat cgctcccatg ccgtttgcca gttgatagcg tccatcttgc
     8941 ccacggcgat gagcaatgcg atcggcaaac gccccggcaa gtagcggcgc gataagcgaa
     9001 ctgtctgcct caccgccgcg tacgtttaag cgttttaaca gttgctgact acgttgctgc
     9061 caggctggtt gattgcgcga aaacgccacg cccaggtcac tattgcccat cagtggcggc
     9121 tcttcgagaa tggcggcaat ttttgccgcg gtagcagctt cgtcgtcgtt cttcgcgcta
     9181 accagcattg ccgctaaacg cggatcgtta cccagcgctg ccattttttg cccttgcgca
     9241 ctaagccgtt caccgtccag cgcccccagc atttgtaaca gacgtttcgc ggccattaga
     9301 ttcactactg gcggttgatc cagccagctc atctgcgccg gatcgctgca tccccattgc
     9361 agtaattcca tcagcaaacc ggaaagatcg ctttgtaaga tctccggttc actttgcgcg
     9421 gcagcgcgtt ctgcttgttc tttggcgatt aaatgcaggc agatacccgg ctccagacgc
     9481 ccggcgcgcc cggcacgctg cgtcatggat gcctggctaa cgcgttgagt aatcagtcgc
     9541 gtaagccccg tgcgcggctc aaaacgcgcc acacgctcct gggcacaatc caccaccaga
     9601 cgaatacctt caatggttaa actggtttca gcaatattgg tcgccagcac cactttgcgc
     9661 atcccttgcg gtgccgggag gatcgctttt cgctgatcgt tcagcgacaa cgcgccatac
     9721 agcgggcaga gcaacacatc actgccgatg cgcgaagcca gttgttcctg cacacgctga
     9781 atttctccga cgccaggtaa aaataacagt aatgatccgc tttcctgacg cagcatttcg
     9841 gcggtggcta ccgcaacggc ttcgtcaaaa cgctgatgcg cgggcagcgg taaataatgg
     9901 cgttcaaccg gaaacgagcg cccttctgag atgacgacgg gcgcttctgg cagcatttgc
     9961 tgcaagcggt cgttatccag cgtagccgac ataatcagca gtttaaggtc atcacgcaga
    10021 ccttgttgca catcgagtaa cagcgccaac gccaaatccg cctgcaagct gcgctcatga
    10081 aattcatcaa ggatcaccaa tccaacaccg ctcaactccg ggtcacgctg gatcatccgc
    10141 gtcagcacgc cttcggtaac tacttccaga cgggtatttg gcccgacgca gttttgcgca
    10201 cgcatccggt agccaacggt atcgcctggc ttttcgttaa gcagctccgc cagccgttgc
    10261 gcgacgttac gtgccgccag acgacgcggc tccagcagga taattttccc gttaatgccg
    10321 ggatgcgcca gcagttgcag cggcagccag gttgatttcc cggccccggt cggcgcactt
    10381 aataatacct gcggcgcaca atcgagagcg gtaagtaatt caggtaagac ggcagcaacg
    10441 ggcaacgacg acacaaaacg ctccagaggg ttaacattct tcgcgccaca ttgtagcatc
    10501 gcggtaattc ataaccgagt gcctcacatg tctgaaccgc aacgtctgtt ctttgctatc
    10561 gacttacctg cagaaatccg cgaacagatt atccactggc gcgccacaca cttcccacct
    10621 gaggcgggac gtccggtcgc cgccgataat ttgcatctga ctctggcatt tttaggcgaa
    10681 gtgagcgccg agaaagagaa ggcgctttct cttttagccg gacggattcg tcaacctggt
    10741 ttcacactca cgcttgatga cgccggacaa tggctgcgtt cgcgtgtggt gtggttaggg
    10801 atgcgtcagc cgccacgcgg cttaatccag ctggcgaata tgctccgttc acaggctgcc
    10861 cgcagcggtt gttttcaaag caatcgtccg tttcatccac atattacctt attgcgcgac
    10921 gccagcgagg cggtgacaat cccgccgcca ggttttaact ggtcttatgc ggtgacggag
    10981 ttcacccttt acgcctcctc gtttgcccgt ggacgcacac gctacacgcc gctaaaacgc
    11041 tgggcgctaa cgcaataaca aggattgtcg caatggaatt ctctccccct ctacagcgcg
    11101 cgacgctaat tcagcgttac aaacgttttt tagccgatgt gatcacaccc gatggtcgcg
    11161 aattaacgct acactgcccg aatacgggtg cgatgaccgg ttgtgcaacg cgtggcgata
    11221 ccgtctggta ttcgacttca gacaacacca aacggaaata cccacacacc tgggaactaa
    11281 ctcaaagcca gagcggcgca tttatttgcg tcaatacgct ttgggctaac aggttgacga
    11341 aagaggctat ccttaatgaa tcaatttcag aactgtcagg ctatagctcg ctgaaaagcg
    11401 aagtaaaata cggcgcagaa cgcagccgta ttgactttat gttgcaggcg gattcgcgtc
    11461 cagactgcta tattgaagtg aaatcggtta cgttagcgga gaacgaacag ggatattttc
    11521 ccgatgcggt cactgaacga ggtcagaaac accttcggga gttgatgaac gtagcggctg
    11581 aaggccagcg tgcggttatc tttttcgccg tgctgcattc agccattaca cggttttcac
    11641 ccgcgcgcca catcgatgag aaatacgcgc aactattgtc agaagctcaa cagagggggg
    11701 tagaaattct ggcttacaaa gcggaaattt ctgctgaagg catggctctt aaaaaatcac
    11761 tgtcggttac attgtagtaa agtaagtaac tggttaattt acattctggt cgcgtgcgca
    11821 aatacgcttt tcctcacaca gttgtcaagt gttacgttta gataattgct atccggaaaa
    11881 gcatctgcta tttatagcgg cctcattttt cccccgaaca tggggatcga tagtgcgtgt
    11941 taaggagaag caacatgcaa gaagggcaaa accgtaaaac atcgtccctg agtattctcg
    12001 ccatcgctgg ggtggaacca tatcaggaga agccgggcga agagtatatg aatgaagccc
    12061 agctggcgca cttccgtcgt attctggaag catggcgtaa tcaactcagg gatgaagtcg
    12121 atcgcaccgt tacacatatg caggatgaag cagccaactt cccggacccg gtagaccgtg
    12181 cagcccagga agaagagttc agcctcgaac tgcgtaaccg cgatcgcgag cgtaagctga
    12241 tcaaaaagat cgagaagacg ctgaaaaaag tggaagacga agatttcggc tactgcgaat
    12301 cctgcggtgt tgaaattggt attcgccgtc tggaagcgcg cccgacagcc gatctgtgca
    12361 tcgactgcaa aacgctggct gaaattcgcg aaaaacagat ggctggctaa ttacagccgt
    12421 tccatcacgt ttaccacagg cgggaaatgc tcccgcctta ttttttgttc aaagagatga
    12481 cagacacaca gtatattggc cgcttcgccc cctctccttc cggcgagctt cattttggct
    12541 ctctgatcgc cgcgctcggc agctatttgc aggctcgcgc ccggcaaggt cgctggctgg
    12601 tacgcataga agatatcgac ccgcctcgtg aagttcccgg tgccgcagaa actatcctgc
    12661 gccagctgga acattacggt ctgcactggg acggcgatgt tctctggcaa tcgcaacgtc
    12721 acgacgccta tcgtgaagca ctcgcctggt tacatgaaca aggactaagt tattactgca
    12781 cctgtacgcg tgcgcgtatt caaagcattg gcggtattta cgacggtcat tgccgggtgt
    12841 tgcatcatgg accagacaat gccgcagtgc gtatccgcca gcagcatccg gtcacgcaat
    12901 ttactgacca gctgcgcggc attattcacg ccgacgaaaa actggcacgg gaagatttta
    12961 tcattcatcg ccgtgatggg ttgttcgcct acaacctggc tgttgtggtt gatgatcatt
    13021 tccagggcgt tacagaaata gtgcgtgggg ctgatctgat tgaaccaaca gtaaggcaaa
    13081 tctcgctgta ccagcttttt ggctggaaag tgccagatta cattcatctg ccgctggcgc
    13141 ttaatccaca aggcgctaaa ctttccaagc agaatcatgc gcctgcgttg ccgaaaggcg
    13201 atccacgtcc ggtactaatc gcggcacttc aatttctggg tcagcaggca gaagcacact
    13261 ggcaggattt cagcgtcgaa caaatccttc agtcagcagt caaaaactgg acgctgaccg
    13321 ccgtgcccga gtcggcaatt gtaaattcaa cattctcaaa tgcgtcatgc tgagctatga
    13381 ttagccgcta tttttttgtc ctgaatgatg tttgacacta ccgaggtgta ctatttttac
    13441 ccgagtcgct aatttttgcc gcaaggtgct aagccgcgag gaaagcgagg ctgaacaggc
    13501 agtcgcccgt ccacaggtga cggtgatccc gcgtgagcag catgctattt cccgcaaaga
    13561 tatcagtgaa aatgccctga aggtaatgta caggctcaac aaagcgggat acgaagcctg
    13621 gctggttggc ggcggcgtgc gcgacctgtt acttggcaaa aagccgaaag attttgacgt
    13681 aaccactaac gccacgcctg agcaggtgcg caaactgttc cgtaactgcc gcctggtggg
    13741 tcgccgtttc cgtctggctc atgtaatgtt tggcccggag attatcgaag ttgcgacctt
    13801 ccgtggacac cacgaaggta acgtcagcga ccgcacgacc tcccaacgcg ggcaaaacgg
    13861 catgttgctg cgcgacaaca ttttcggctc catcgaagaa gacgcccagc gccgcgattt
    13921 cactatcaac agcctctatt acagcgtagc ggattttacc gtccgtgatt acgttggcgg
    13981 tatgaaggat ctgaaggacg gcgttatccg tctgattggt aacccggaaa cgcgctaccg
    14041 tgaagatccg gtacggatgc tgcgcgcggt acgttttgcc gccaaattgg gtatgcgcat
    14101 cagcccggaa accgcagaac cgatccctcg cctcgctacc ctgctgaacg atatcccacc
    14161 ggcacgcctg tttgaagaat cgcttaaact gctacaagcg ggctacggtt acgaaaccta
    14221 taagctgttg tgtgaatatc atctgttcca gccgctgttc ccgaccatta cccgctactt
    14281 cacggaaaat ggcgacagcc cgatggagcg gatcattgaa caggtgctga agaataccga
    14341 tacgcgtatc cataacgata tgcgcgtgaa cccggcgttc ttgtttgccg ccatgttctg
    14401 gtacccactg ctggagacgg cacagaagat cgcccaggaa agcggcctga cctatcacga
    14461 cgctttcgcg ctggcgatga acgacgtgct ggacgaagcc tgccgttcac tggcaatccc
    14521 gaaacgcctg acgacgttaa cccgcgatat ctggcagttg cagttgcgta tgtcccgacg
    14581 tcagggtaaa cgcgcatgga aactgctgga gcatcctaag ttccgtgcgg cttatgacct
    14641 gttggccttg cgagctgaag ttgaacgtaa cgctgaactg cagcgtctgg tgaaatggtg
    14701 gggtgagttc caggtttccg cgccaccaga tcaaaaaggg atgcttaacg agttggatga
    14761 agagccgtca ccgcgtcgcc gtactcgtcg tccacgcaaa cgcgcaccgc gtcgtgaggg
    14821 taccgcatga cagtggcgta tattgccata ggcagcaatc tggcctctcc gctggagcag
    14881 gtcaatgctg ccctgaaagc attaggcgat atccctgaaa gccacattct tgccgtttct
    14941 tcgttttacc gcactccacc gctggggccg caagatcaac ccgattacct caacgccgcc
    15001 gtggcgcttg aaacttcgct cgccccggaa gagttactca accatacaca gcgcatagaa
    15061 ctgcaacagg gtcgtgttcg taaagctgaa cgctggggac cacgcacgct ggatctcgat
    15121 atcatgctgt ttggtaatga agtgataaat accgaacgcc tgaccgttcc gcactacgat
    15181 atgaagaatc gtggatttat gctgtggccg ctgtttgaga ttgcaccgga gctggcgttt
    15241 cctgatgggg agacgttgcg tgaagtctta catacaagag catttgacga attaagcaaa
    15301 tggtaattta tttatattac gaatataaat attttctgaa aatcatacat cctctctttc
    15361 cttaataaaa attttagttt aaacattttt gcagaaacat aatataaatt cccgcagata
    15421 aatatcaatc ccatcgccat accctaacca attgcacttg tgaatttatt tttgcttaat
    15481 tttgaattaa gaaaatttaa tttgttcaaa agcccttaaa gtgcacaata ggcgtcctaa
    15541 tgaacaatgg ttgtaaattt tggtaacaaa tatcattgct cattaatatc tagcatctgc
    15601 ttataacatt tgtgtcatga atagtcattc ttcaaaccag tagtctgact aatggaattt
    15661 tattcaagga tttttatgtc taaaaaatta ggttttgccc tgtctggtct tatgctggca
    15721 atggttgcag gtactgcttc tgctgatatg gatggtggtc agttaaatat cagtggtctg
    15781 gttgttgata acacctgtga aacccgcgtt gacggcggta ataaagatgg tctgatcctg
    15841 ctgcaaactg caactgttgg tgaaatcgat gctggcgtgc tgaatgacac cgttggcgct
    15901 aaagccaaac ctttcagcat caccgttgac tgcagcaaag caaatccgaa tccaggtagt
    15961 acagctaaaa tgaccttcgg ttctgttttc tttggtaata gcaaaggaac gctgaacaac
    16021 gacatgtcta ttaacaaccc gtctgacggc gtgaatatcg ctctgcacaa tattgatggt
    16081 tccactatca aacaggttca aattaacaac cctggcgatg tgtatactaa agccctggat
    16141 gcaacgacaa aatctgctgt ttatgatttt aaagcgtctt acgttcgtgc cgtcgcagac
    16201 caaacagcaa ctgctggtta tgtaaaaact aacactgcat acaccattac ttatcagtaa
    16261 gtattcacca attaaattga ttgaatgaat atacagggaa tagtgttaat aatttctatt
    16321 tttatattat tccctgtttt aattaactct gtcagggatg gtttatgttt tttaacacca
    16381 aacatacaac agctttatgc ttcgtaacct gtatggcttt tagttcatcg tctattgcgg
    16441 acattgtcat ttcgggtact cgcgtaatat ataaaagcga tcaaaaaagt gtcaacgtac
    16501 gtctggaaaa taaagggaat aacccgttgc ttgtccagag ttggttagat actggcgata
    16561 acaacgctga gcctggcagt attacagtcc cttttactgc cacgccgcca gtatcgcgta
    16621 ttgatgccaa acgtgggcaa acaatcaaat taatgtacac agccagcacc tcactgccta
    16681 aagacagaga gagcgtgttc tggtttaacg tactggaagt tccaccaaaa ccagatgcag
    16741 aaaaggtcgc gaatcaaagt ctgctgcaac tggcatttcg cacacgtata aaacttttct
    16801 atcgcccgga tggattgaag ggaaatccct ctgaagcccc gttagccctg aagtggttct
    16861 ggtcaggttc agaaggtaag gcgtcattgc gagtgaccaa tccaacccct tactacgtct
    16921 cttttagcag tggtgattta gaagctagcg gtaaacgcta tccgattgat gtgaaaatga
    16981 ttgcaccatt tagtgatgag gtcatgaaag tcactggcct taatggcaaa gcaagttctg
    17041 caaaagtaca tttttacgcc attaatgatt ttggtggcgc cattgaaggt aatgccagtc
    17101 tgtaatcagg caaggatata attccgcagg aagcataacg tgactataaa accaactaaa
    17161 aattatcata atcatctgac ccgtatcgct acgttttgcg cgctgctgta ttgcaatagt
    17221 gctttctgtg ctgaacttgt tgaatatgac cataccttcc tgatggggca gaatgcctca
    17281 aatattgatc tcagccggta tagtgaaggt aaccctgcta taccgggtat gtacgacgtc
    17341 agtgtttatg tcaacgatca acctattatt aaccaaagca ttacatttat tgaaattgaa
    17401 ggaaaaaaaa cgcacaagcc tgtatcacac ttaagaattt attgcagttt catataaatt
    17461 ctcccgatat aaataacgaa aaagccgttc tgcttgccag ggatgaaaca ctcggcaatt
    17521 gccttaattt gacggaaatt atccctcagg cttctgttcg ttatgacgtt aacgagcaac
    17581 gtctggatat agacgttcct caggcatggg taatgaaaaa ttaccaaaac tatgttgatc
    17641 catcgttatg ggaaaatggc attaatgcgg ccatgttgtc atacaacctc aacggatatc
    17701 atagtgaaac tcctggtcga agaaatgaca gcatttatgc tgcttttaac ggtgggatga
    17761 atttaggtgc atggcgactg cgtgcctcag gcaactacaa ctggatgacc gattctggca
    17821 gtaattatga ttttaagaat cggtatatac agcgtgatat cgcctcgctg cgttctcaac
    17881 tcattcttgg tgagtcttat acaacgggcg aaacctttga ttctgtcagt atccgcggca
    17941 ttcgtttata cagcgacagc cgcatgttgc ctccgacatt agcgagtttt gcgcctatca
    18001 ttcatggcgt tgctaatacc aacgccaaag tcactattac gcaaggtggc tataagattt
    18061 atgaaacgac tgtgccgcca ggcgctttcg tcattgatga tctgagtccg tcaggttacg
    18121 gcagcgatct tattgttact gtcgaagaat ccgatggctc aaagcggaca ttctcgcaac
    18181 ctttttcatc ggtcgttcaa atgttacgcc ctggcgttgg acgttgggat attagcggcg
    18241 gccaggtctt aaaagatgat attcaggatg agcctaattt atttcaggcc agctactact
    18301 acggtctgaa taactatcta ac
//
LOCUS       DAJTZL010000079        18211 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.079, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000079 DAJTZL010000000
VERSION     DAJTZL010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18211
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.079"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            15..1091
                     /locus_tag="OO544_002311"
     CDS             15..1091
                     /locus_tag="OO544_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HCR6767894.1"
                     /translation="MQRKKYDPNLPRNLTYRRRDKAYYWRNPLTKEEFTLGKISRRDA
                     VTQAIEANHYIYKNYSPAALIEKLKGFDSFTMADWIERYKTILIRRKVSRNTYKIRAN
                     QLETIKEKLGEILLTEITTRHIAEFLDLWIEGGKNTMAGSMRSVLSDMFREAIVEGRI
                     SQNPVTPTRAPKIVVTRERLKLKIYNCIREAADQLPAWFPLAMDLALVTGQRREDITN
                     MRFSDIYDDRLHVRQIKTGMMIAIPLSLSLPVAGLRLGTVVERCRLVSRGDYLISAGI
                     RKNSPDGSIHPDGLTKKFVAARKFTGIQFSENPPTFHEIRSLAGRLYKETCGEEFAQR
                     LLGHTSEKTTKMYLDEREKTYLLL"
     gene            complement(1484..1825)
                     /locus_tag="OO544_002312"
     CDS             complement(1484..1825)
                     /locus_tag="OO544_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HCR6767895.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTNAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(1838..2710)
                     /gene="copD"
                     /locus_tag="OO544_002313"
     CDS             complement(1838..2710)
                     /gene="copD"
                     /locus_tag="OO544_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HCR6767896.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLTIRRLLAKRFL
                     RLQQHAAVWSLISATAMLAVQGGLMGTGWTDVFSPNIWQAVLQTQFGGVWLWQIVLAL
                     VTLIVALMQPRNMPRLLFMLTTAQFILLAGMGHATLNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMCFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTYWGQLLLLKAILVMIMVVIALANRYVLVPRMRQEEDRAAPWFVWMTKLEWAIGAV
                     VLVIISLLATLEPF"
     gene            complement(2714..3088)
                     /gene="yobA"
                     /locus_tag="OO544_002314"
     CDS             complement(2714..3088)
                     /gene="yobA"
                     /locus_tag="OO544_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HCR6767897.1"
                     /translation="MASTARSLRYALAILTTSLVTPFVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            3227..3457
                     /gene="holE"
                     /locus_tag="OO544_002315"
     CDS             3227..3457
                     /gene="holE"
                     /locus_tag="OO544_002315"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HCR6767898.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            3559..4215
                     /locus_tag="OO544_002316"
     CDS             3559..4215
                     /locus_tag="OO544_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HCR6767899.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPEGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEYGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            4239..4901
                     /gene="exoX"
                     /locus_tag="OO544_002317"
     CDS             4239..4901
                     /gene="exoX"
                     /locus_tag="OO544_002317"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HCR6767900.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(4898..6958)
                     /gene="ptrB"
                     /locus_tag="OO544_002318"
     CDS             complement(4898..6958)
                     /gene="ptrB"
                     /locus_tag="OO544_002318"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HCR6767901.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWEILLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPCENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTDERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDNHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(7167..7826)
                     /locus_tag="OO544_002319"
     CDS             complement(7167..7826)
                     /locus_tag="OO544_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HCR6767902.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVRDGIERDLEQQKRTLA
                     E"
     gene            complement(8153..8509)
                     /locus_tag="OO544_002320"
     CDS             complement(8153..8509)
                     /locus_tag="OO544_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HCR6767903.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(8576..8866)
                     /gene="yebG"
                     /locus_tag="OO544_002321"
     CDS             complement(8576..8866)
                     /gene="yebG"
                     /locus_tag="OO544_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HCR6767904.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            9000..10178
                     /gene="purT"
                     /locus_tag="OO544_002322"
     CDS             9000..10178
                     /gene="purT"
                     /locus_tag="OO544_002322"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HCR6767905.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSESLFREAVADIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWKYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVALATAESVVDAIERAKHAAGQVKVQG
                     "
     gene            complement(10234..10875)
                     /gene="kdgA"
                     /locus_tag="OO544_002323"
                     /gene_synonym="eda"
     CDS             complement(10234..10875)
                     /gene="kdgA"
                     /locus_tag="OO544_002323"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HCR6767906.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(10912..12723)
                     /gene="edd"
                     /locus_tag="OO544_002324"
     CDS             complement(10912..12723)
                     /gene="edd"
                     /locus_tag="OO544_002324"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HCR6767907.1"
                     /translation="MNPQLLCVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSLGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEQ
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(12958..14433)
                     /gene="zwf"
                     /locus_tag="OO544_002325"
     CDS             complement(12958..14433)
                     /gene="zwf"
                     /locus_tag="OO544_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HCR6767908.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            14771..15640
                     /gene="hexR"
                     /locus_tag="OO544_002326"
                     /gene_synonym="yebK"
     CDS             14771..15640
                     /gene="hexR"
                     /locus_tag="OO544_002326"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HCR6767909.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAAMALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRTVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            15768..17210
                     /gene="pyk"
                     /locus_tag="OO544_002327"
     CDS             15768..17210
                     /gene="pyk"
                     /locus_tag="OO544_002327"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HCR6767910.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(17270..18112)
                     /locus_tag="OO544_002328"
     CDS             complement(17270..18112)
                     /locus_tag="OO544_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HCR6767911.1"
                     /translation="MHHIKTAADLNCFLNSMVAVSQPTVDKIIFGAEATLINKIGTCW
                     IASMDVLRKAVFEGVNIIITHEPTFYSYADLEGDDLEFSWARKIMDYTRGELSYLKII
                     EQKKEFLHKNNLVIIRCHDVMDREPTFGMSKALAQQLELDVTNIVASDEMYCVYAIDP
                     DSAINITKRFAKNLKIYNLPGIQFYGDKARVVRTVGIGAGCFCDPIQYMEYQADYYIT
                     INDSIKTWVQTQYSKDSGLPMAVIGHSVAEEAGMRRLASYLDLHSGYPCIHFTGGCDY
                     DWIE"
BASE COUNT         4670 a         4574 c         4391 g         4576 t
ORIGIN      
        1 ggagaagtta aaacatgcag cggaaaaaat acgatcccaa tttgcctaga aacttaacat
       61 atcgaaggag agacaaagca tattactggc gcaaccctct gacgaaagaa gaatttacac
      121 taggtaaaat ttcaagaaga gatgcagtaa cgcaggcaat tgaagcaaat cattatatat
      181 acaaaaacta ctctcccgct gccttaattg aaaaacttaa agggttcgac tcatttacta
      241 tggcagactg gattgaacgt tacaaaacga ttcttataag gagaaaagtg tccagaaata
      301 cttataaaat tcgggcaaat caactggaga caataaaaga aaaattggga gagattttac
      361 tgacagaaat aaccactcgc catattgccg agtttcttga tttgtggatt gaaggaggga
      421 aaaacacaat ggcaggatca atgcgttctg tgttgtctga tatgtttcgc gaggccattg
      481 ttgaaggacg tatatctcaa aatccagtca cgccaacaag agcaccgaaa atagtagtta
      541 caagagaacg actgaaacta aagatataca actgcatcag ggaggcagca gatcaacttc
      601 cggcatggtt cccattagct atggatttag cccttgtaac aggacaacgt cgcgaagaca
      661 taacgaatat gcggttcagt gatatttatg atgatcgtct ccacgtcagg caaattaaga
      721 caggaatgat gattgctatc cccctgtcac tcagccttcc tgtcgctggt ctacggcttg
      781 gtacagtagt tgaacgatgc cgcctggtaa gccggggaga ttatctaatc agtgccggga
      841 ttagaaaaaa cagccctgac ggcagcattc acccggatgg cctgacaaaa aaatttgtcg
      901 cagccagaaa attcacaggt atccagttca gtgaaaaccc accaactttt cacgagatca
      961 gaagcctggc tggacgattg tacaaagaaa catgtggaga agaatttgct cagcgtctac
     1021 ttggccacac atcggagaag acaacaaaaa tgtatcttga tgagagagaa aaaacgtact
     1081 tactgctctg attttaacgt aaatggattg ttaaatgtat tttggttgtg atataaccaa
     1141 aaagaccgga atacagaaat tcgagtaaat ttcggggaat ttcgggggag acatttgcaa
     1201 ctaattgatt tcaaatgcaa ttaaaaaaag accgaatacg attcctgtat tcggtccagg
     1261 gaaatggctc ttgggagaga gccgtgcgct aaaagttggc attaatgcag gcttagttgc
     1321 cttgcccttt aagaatagat gacgacgcca ggttttccag tttgcgtgca aaatggtcaa
     1381 taaaaagcgc ggtggtcatc agcttaaatg ttaaaaaccg cccgttctgg tgaaagaact
     1441 gaggcggttt ttttattgga aatcaaaagg ctattttagg taattaacag agtttttcag
     1501 ctcgttctat aaacggtgcc agactcattt tttcgccggg attgttagga tcatcaatct
     1561 gaatcaccga aatgggttgg gcattggtct tcccactggc aacttccttt tgtgcgatat
     1621 cgtttaaagg atactgcacg agggtactcg gattaataac atacaaagca ttacccggtc
     1681 ggcaagtcag catcacctct tcgcgattaa acgcccattt gtctttaccc acttcaaaac
     1741 gactgacggt aatcacctgc ggtgcagcca gcgccgctgc agaactggtg agtaacagaa
     1801 acgccagaat actttttttc atcattttcg taatccatca aaaaggttcg agagtcgcaa
     1861 gcaggctgat gatcaccagc actaccgcac ctatcgccca ttcgagttta gtcatccaca
     1921 caaaccaggg tgccgcacga tcttcctcct gcctcatgcg cggtacaaga acataacgat
     1981 tagccagcgc aatgaccacc ataatcatca ccaggatggc tttgagcaat aagagttggc
     2041 cccagtaagt cgtaagcgtg gggggaaatc cggtaatcaa caatgcatta agcacgccgc
     2101 ttgccagtac gccgatcacc gcaaaatgcc cgcaccatga aaaacacatc agcgcctgaa
     2161 tagcctggtg tcgccaacgg cctttgatga gttgcataca ccagagcacc ggcagtaatc
     2221 ccccaaacca cgccgccgca caaatcaggt gaatcgcgtg attagtctga tggattttcg
     2281 ccgttacccc ttcattcagt gtcgcatgcc ccattcccgc cagcagaata aattgcgcgg
     2341 tagtgagcat aaacagcaaa cgtggcatat tccgcggttg cataagggcg acgatcaacg
     2401 ttacgagggc gagaacaatt tgccataacc agacgccacc aaactgcgtt tgcaaaacag
     2461 cctgccaaat gtttggcgaa aatacatctg tccatcccgt tcccatcaac ccgccctgaa
     2521 cggcgagcat tgcagtggca ctgatcaaac tccagacggc agcgtgctgt tgtaaacgca
     2581 gaaaacgctt cgccagcaag cgacgaatcg tcaagggggc cagccaggca ccgtacattg
     2641 cgaagccaaa aaccagcatc agcgaggtga aatggataaa tcgcagcgcg atccaggtaa
     2701 acgccagcat gatttatttc acgctaaagg tgtagtgtcc tttcgtttta tgcccgtcca
     2761 ccgaaacaac atgccagtca acggtgtagg ttccgggttt cagcgaatcg gccagtggaa
     2821 caatcagttg cttttggtcc tgctcatttc gcttcgccgg taatgtttta atattttcgt
     2881 tttttggccc cgtgattttt gcaccactga atcctgtttc cacaccttcc gagaagttta
     2941 aagtgattgc ctgcggtgca gctgtcactt gcgcgtttgc cgcaggatac tgatgcgtta
     3001 aatgcgcatg tgcccagacg aaaggtgtca ccaacgaagt ggtcaggatt gccagcgcgt
     3061 agcgaaggga gcgtgcagtt gaagccatat tatctattcc tttttgtaat aactttttta
     3121 cagagcataa ccttgtctaa tgtctgagtc gaggatcatc aattccggct tgccatcctg
     3181 gctcactctt agtaactttt gcccgcgaat gatgaggaga ttaagaatgc tgaagaatct
     3241 ggctaaactg gatcaaacag aaatggataa agtgaatgtc gatttggcgg cggccggggt
     3301 ggcatttaaa gaacgctaca atatgccggt gatcgctgaa gcggttgaac gtgaacagcc
     3361 tgaacatttg cgcagctggt ttcgcgagcg gcttattgcc caccgtttgg cttcggtcaa
     3421 tctgtcacgt ttaccttacg agcccaaact taaataaaac ttatacaggg ttacactttc
     3481 ttacataacg cctgctaaat tatgagtatt ttctaaaccg cacccataat ttgcagtcat
     3541 tttgaaaagg aagtcattat gtcgttctgg aaagttgcag cagcgcaata tgagcctcgt
     3601 aaaacctcac tcaccgaaca agtagctcat catctggagt ttgtgagggc cgccgccagg
     3661 caacaatgtc agctgttagt ttttccatcg ctttctttac tggggtgtga ttattcgcga
     3721 cgtgcccttc ctgccccacc cgatctctca ctgttagacc cgctttgtta tgccgcaacg
     3781 acctggcgaa tgaccatcat tgccggcctt cccgttgaat ataacgatcg ctttattcgt
     3841 ggaattgcgg tgttcgcacc gtggcgaaaa acgcctggga tctaccatca gagtcatggt
     3901 gcatgtctgg gccgtcgttc cagaaccatc acggtggtcg atgaacaacc ggaaggtatg
     3961 gatatggacc cgacctgttc actgtttacg acagggcaat gtctgggaga acccgacctg
     4021 ctggcgtcgg cccgtcgctt acaatttttt tcacatcagt actcgattgc tgtgctgatg
     4081 gcaaatgccc gtggtaacag tgcactatgg gatgaatatg gtcgtctcat cgttcgcgcc
     4141 gatcgtggtt cattattgtt agtcggtcag cgttcatcac agggttggca aggcgatatc
     4201 attccattac gctaggcttt ttcggcctgg agtatgccat gttgcgcatt atcgatacag
     4261 aaacctgcgg tttgcaggga gggatcgttg agattgcctc tgttgatgtc attgacggaa
     4321 aaatcgtcaa ccccatgagc cacctggtgc gccccgatcg tcctattagt ccacaagcga
     4381 tggcgattca tcgcatcacc gaagccatgg tcgccgataa accgtggatt gaagatgtga
     4441 tcccacacta ttacggcagt gaatggtatg tcgcgcacaa cgccagcttt gaccgccgcg
     4501 tactgcctga gatgcccggt gagtggattt gcaccatgaa actggcccgt cgcttgtggc
     4561 caggaatcaa gtacagcaat atggcgttat ataaaacacg gaagctcaat gtacagacgc
     4621 cgccgggcct gcatcatcac cgcgcgttgt atgactgtta tatcaccgcc gcgttgctta
     4681 tcgatattat gaacacctcc ggctggacgg cagaacagat ggccgatatc accggacgtc
     4741 cgtcgttgat gacgaccttc acctttggca aataccgtgg caaagcggtt tccgacgttg
     4801 ccgaacgcga tccgggctat ctgcgctggt tatttaataa cctggacagc atgagcccgg
     4861 agctgcgttt aacactgaaa cattatctgg aaaatactta atccgcaggc gtagcgggta
     4921 atgttccctg cgccagcgcg accagaaaag catattccat cgctacgcct tcgtacgatt
     4981 taaagcggcc agatttaccg ccatggcctg agtccatgtc ggtacagagc aataaaagat
     5041 ggttatcggt tttcagctcg cgcaatttag cgacccattt tgccggttcc caatattgca
     5101 cctgagaatc gtgtaaaccg gtcgttacca gtaaatgcgg ataagcctgt gcggtgacgt
     5161 tgtcatacgg gctgtagctt ttcatgtact cgtaatattg cggatcctgc gggttacccc
     5221 actcttcaaa ctcaccagtg gtaagaggaa ttgattcatc aagcatcgtt gttacaacat
     5281 caacaaacgg tacctgggcg ataacgccgt ggaataattc cgggcgttga ttaattgcaa
     5341 cgcccatcaa catgcccccc gcactcccgc ccatcgcata acaaagcgaa ggagagccat
     5401 agcccagttt taacaatgca tcgcaggcat caagataatc attaaacgta tttttcttct
     5461 tcagaaattt tccgtcttcg taccattgtt gccccagctc accaccgccg cgaacatgga
     5521 caatggcgta gacaaagcca cgatctaaca aactcaagcg gctaaaactg aaatcggcat
     5581 caatacttgc gccgtaagaa ccatagccat acaccagcaa cgggttgtgt cctttgcgaa
     5641 aatgtttgcg atggtagacc aacgaaaccg gaacttcgac gccatcacgg gcgactatcc
     5701 acaggtgttc actgcggtaa ttcgccgcat caaaaccagg aacttccgtt tgttttaata
     5761 cacgacgctc atcggtatcc atatccagtt caaacaaagt gtctggtgtg gtcatggaag
     5821 aataaccata acgcaatcgc gcggtttcag gttctggatt gtaggcaatc caggtcacat
     5881 aggctggatc atcaaaggca ataccaatga cttcccgggt cttgcggtta atttggcgca
     5941 aactggttaa cccgcgctga cgctcttcaa ccaccagcca gtcggtaaac agcgtaaacc
     6001 cttccagcat gatgttttcg catggcggaa ttaactcttc ccactgttgc tcatcacgca
     6061 tacgggtacg gtataagcca aagtttttgc cgtggcggtt ggaacgcaga taaaaacgat
     6121 gctggtagtg atcaaggctg tattcgtgat ctttgcggcg cggcagaaaa acaaacggct
     6181 cggcatcggc catttccgcg tccagcaggc gaacttcact ggtggtggcg ctggccaaat
     6241 gaatgactac atagtgcttc gaggtcgttt tatgcaggct gacgtaatag gtatcgtctt
     6301 tttcttcgta gatcagttta tcttgcgatg ctggcgtacc gatggcgtga cgccagacct
     6361 gataaggcag cagcgtcacc ggatgcttgc gaacatagta gaaagtccag gagtcatttg
     6421 cccagacaaa gctgggttca acgttatcca gcagttccgg gtaccagtta ccagtttcca
     6481 gattacgaaa acgaatgccg tactggcgtc gggaaagaaa atcttctgcc agcgccataa
     6541 tggtgttatc gggcgtaatc gccattccgc ccatcgaata aaactcacta tgagctgcgc
     6601 gcttattggc atcgagcaat atttcccact catcccactc ttcactgaat gccgattgac
     6661 gctggtagat agcatattca cagcctggtt cataaatatg ccgatagcgg tagccatttt
     6721 tgatgtaggg cgcagaaact tctcgttgcg gaatgcggtc gatgatttcc tttaagatgc
     6781 gatcctgcaa ggcttgttgt gaggccatca cccgatgacc gtaactattt tcttgttgca
     6841 ggtagtccag gacttctggc tgagaacgcg tatcatcccg cagccagtag taattatcga
     6901 tgcgcgtatc gccatgaagc gtcatggcgt ggggaatgcg ggcggctttt ggtagcatgt
     6961 tattgttctt tctggttgaa acatcttata agggtggcaa aactcaccgg ggatgcaagc
     7021 gaaacagggg agtcattgct tagatgatga caggtaatgg cacggatatc gaatgttatg
     7081 cagacagaga aactcagcct gttcaaatgg ctgtgcggtt ctggatagcc cgaaatagtc
     7141 aacttcaggc tatccagaga gcggaattat tccgccaaag tgcgtttttg ctgttcgaga
     7201 tcgcgttcaa tgccgtcacg aacatcctgg gggattttca gcgcgtcacc cagtgcattc
     7261 aggtaactgc gttccataaa atggtcaata tcaatagccg cgcaactcag gaaatagatt
     7321 tccagcgcct cttcttcatt gcggaccccg gtagccaggc gttgtggatc cagcggttgt
     7381 tcgattgcct gctcaatgag tacacgcccc tgctcttcca cgccggcttc acgcaattgc
     7441 tggtcgattg ccgcacgttc tttggcatca atatgaccat cacttttagc ggcaaagacc
     7501 agcgcaagga tcaaacgttc tgtacgctca tccagcggcg tactttgcgc gccaaactgc
     7561 ggttcgtcct gatgcgccgc gcgaatttta tctttgtatt tattccacag caccgtaccc
     7621 gctaccgctc cgccgccaac cagtaacgcg ttggtgccat atttcgtaag aagtttacgt
     7681 gctgatttat ttgcgaccag cagcccagcc agtccgccta atgcgcctgg cactaacagt
     7741 ttgcccaatc cctgatccgc agacgaggag gtagaagaac tgctttgccc aagcagggat
     7801 tgcagttgat ttaaccagtt agccatattt gctcctcaat aaccattttt agtgcctgta
     7861 gcatagcgga tgaggatgtc agaaaatgta tatcgagaca aaagatgcgc aaataagcgg
     7921 ggcggtaggc cgttattcaa agaaattcgc gccaggtaag gtatttactg tatatcaggt
     7981 gataagggta ttttaggtga aagtagtttg tcgttttgag tggcattgtt tgatgctgat
     8041 tgcccgatgc gacgctgttg cgtcttctca ggcctacgat tgtggtgcgt tctggggcgg
     8101 acaaggcact cgcgccgcat ccaccaacgg tcaatcgaat tatcagaagc cattaacgcc
     8161 gctgatattc cgccattccc gctttgcagt ccacgctgac ctggtaatga atatcggcac
     8221 ttttaccacg cacggttagc ggtactgacc atttatcatc tttaccctga atgtcctgca
     8281 aactgaccca agccacggga tcggcctgac cgacaatttt ttgatcatct gcccaacgcg
     8341 ccacgcgatt ttgttgataa tcacgtttta cgctcgcggc aattccggca gcatccagac
     8401 cttcacactt tgggaaagtg accgacttgc tggtttcatt attggcagcg aaaactgatg
     8461 cgcaggcaga aaccaacaac agccctaaaa acgcccctct ttttttcatg tttttctcca
     8521 tagcacaatg attcaggaga aagcatggta caaattgtca ggagcgcaag ttgcttcagg
     8581 ccgcgtgtga ggcatcttcc tcttcggatt cagcaccgac gacctgtggg gacggtaatt
     8641 tgccggtttt cagaatggtg ctcagcacat ctttttgttc tgccagccat agcgaaaggg
     8701 cttcacgttg ctcgtcttcc atttgcactg gagaatttgt cagccaggtg tcaagcagat
     8761 ccgccgtatc aagcattttg tcataagcat cggcttcctt tttgctggta aacgacattt
     8821 tctcttcgcc ctcacgaatg actacgtatt taacttcaac cgccatttgc agcctctcat
     8881 aataactgtg attttataca gtatatttct tttcggttga gaaatcaaca tcagcaataa
     8941 agacacacgc aaacgttttc gtttatactg cgcgcggaat taatcagggg atattcgtta
     9001 tgacgttatt aggcactgcg ctgcgtccgg cagcaactcg cgtgatgtta ttaggctccg
     9061 gtgaactggg taaagaagtg gcaatcgagt gtcagcgtct cggcgtagag gtgattgccg
     9121 tcgatcgcta tgccgacgca ccagccatgc atgtcgcgca tcgctcccat gtcattaata
     9181 tgcttgatgg tgatgcatta cgccgtgtgg ttgaactgga aaaaccacat tatatcgtgc
     9241 cggagatcga agctattgcc accgatatgc tgatccaact tgaagaggaa ggactgaatg
     9301 ttgtcccctg cgctcgcgca acgaaattaa cgatgaatcg cgagggtatc cgtcgcctgg
     9361 cggcagaaga gctgcagctg cccacttcca cttatcgttt tgccgatagc gaaagccttt
     9421 tccgcgaggc ggttgctgac attggctatc cctgcattgt aaaaccggtg atgagctctt
     9481 ccggcaaggg gcagacgttt attcgttctg cagagcaact tgctcaggca tggaagtacg
     9541 ctcagcaagg cggtcgcgcc ggagcaggcc gcgtaattgt tgaaggcgtc gttaagtttg
     9601 acttcgaaat taccttgcta accgtcagcg cggtggatgg cgtccatttc tgtgcaccgg
     9661 taggtcatcg ccaggaagat ggcgactacc gtgaatcctg gcaaccacag caaatgagcc
     9721 cgcttgccct tgaacgtgcg caggagattg cccgtaaagt ggtgctggca ctgggcggtt
     9781 atggtctgtt tggtgtcgag ctgtttgtct gtggtgatga ggtgattttc agtgaggtct
     9841 cccctcgtcc acatgatacc gggatggtga cgttaatttc tcaagatctt tcagagtttg
     9901 ccctgcatgt acgtgccttc ctcggacttc cggttggcgg gatccgtcag tatggtcctg
     9961 cagcttctgc cgttattctg ccacaactga ccagtcagaa tgtcacgttt gataatgtgc
    10021 agaatgccgt aggcgcagat ttgcagattc gtttatttgg taagccggaa attgatggca
    10081 gccgtcgtct gggtgttgcg ctggcgaccg ctgagagtgt tgttgacgcc attgaacgcg
    10141 cgaagcacgc cgccggacag gtaaaagtac agggttaaac ccggcaaaaa aacgctacaa
    10201 aaatgcccga tcgtagatcg ggcgttttga cttttacagc ttagcgcctt ctacagcttc
    10261 acgcgccagc ttagtaatgc ggtcgtaatc gcccgcttcc agcgcatctg ccggaaccag
    10321 ccaggaacca ccgatgcaca gcacgctttt cagcgccagg tagtcacggt agttagccgg
    10381 agaaatacca cccgtcgggc agaaacggac ctgggagaac ggacccgcga tcgcctgcag
    10441 ggctttcacg ccgccgttag cttcagccgg gaagaatttg aactctttca aaccgtagtc
    10501 catacccagc atcagttcgg aaacagtgct gatccccgga attagaggaa tagtcccttc
    10561 ggtagcagct ttcagcagcg gctcggtcag acccgggcta attgcgaact gtgcacccgc
    10621 ttcagtgact tctgccagct gctgtggatt cagcaccgta ccggcaccca caatcgcttc
    10681 aggcacttct ttggcgatag cacggatagc gtcaactgca cactcggtac gcagagtcac
    10741 ttccagaacg cgcaccccac cagcaaccaa cgcttttgcc atcggcactg cgtgttccag
    10801 tttttttacc acgataaccg gtacaaccgg gccggtggtc aggattgatt ctgcacttgt
    10861 tttccagttt ttcatcagag ttttctctcg cctgattaca aatttgtcgt cttaaaaagt
    10921 gatacaggtt gcgccctgtt cggcaccgga cagtttttca cgcaaggcgc tgaataattc
    10981 acgtcctgtc cccacgcgtg acgcgctcag gtcaggaatg tgcggttcgc gagcagccag
    11041 ttccgcttcg tctaccagca gcgtcagttc gcctgtctgt ccattcacac gaatgatgtc
    11101 cccgtcgcgc acttttgcca gcagcccgcc atcgtaggct tctggtgtta cgtggatagc
    11161 tgacggcact ttacctgaag cgccggagag tcgtccatcg gtaactaacg caattttgaa
    11221 acaccggtcc aataatacac caagtggcgg catgagttta tgtaattctg gcattccgtt
    11281 cgcttttggc ccctgatgac ggacaacaac gacacaatcg cggtccagca aacccgcttc
    11341 aaaggccggc ataacgtcat gctggctttc aaaaacaacc gctggcgctt caatcacctg
    11401 gttctcaacc ggcacggcag aggttttcat aaccgcacgg cccaggttac cgcttaacac
    11461 ttttgtccca ccatgatgag agaaaggttg ttcgaaggaa gcgatcacat tgctgtcgag
    11521 tgatttttcc gccccttccc gccagtcctg ttcaccatta ttcagccatg gttcaagggt
    11581 ataacgagac agaccaaagc ctgccaccgt attgacatct tcatgcagca ggcctgcttt
    11641 gagcagttca cgcaccagaa ccggtacgcc acctgccgcc tggaagtggt taatatcggc
    11701 cggaccgttc gggtagagac gtgccatcag cggtacaaca tcagaaaggt cagagaagtc
    11761 atcccagtta atctgaatac cggccgcgcg cgccatcgcc accaggtgca tggtgtggtt
    11821 agtggaacca ccggtcgcca gcagtgcaac gataccgttc accaccactt tctcatcgat
    11881 catcttaccg atcggcatcc attcattacc attaccggtc atgcgtgtaa cctgacgcgc
    11941 agcggcagcg gtcaaagcat cgcgcagcgg agaatccgga tgaacaaaag aagagcctgg
    12001 caactgcatc cccataaatt ccaccaccat ctggttggtg ttggcagtac cgtagaaagt
    12061 acatgttccc ggcgcatggt aagacgcggc ttctgactcc agtaaggcca tgcggtccac
    12121 tttaccttcg gcataaagct ggcgaatacg cactttttct ttatttggca aaccgcttgc
    12181 catcggtcca gacggcacaa acaccgcagg caaatgacca agcgacaggg ctgccatcgt
    12241 cagacccggg acaatcttgt cgcacacacc gaggaacaga gcaccatcaa acatgttatg
    12301 ggacagcccc accgccgcag acatcgctat cacttcgcgg cttagcagcg acaattccat
    12361 tccatcctgc ccctgggtga caccatcaca catcgccgga acaccgcccg caacctgacc
    12421 aaccgcattc gcttcatgca gggctttacg aatgatttct ggatagtgtt cataaggctg
    12481 gtgcgcggag agcatgtcgt tataggaggt gatgatggcg atattgttac gcaacatgct
    12541 tttcaaagag gctttgtctt ctggctggca ggcagcgaaa ccgtgtgcca ggttaccgca
    12601 tgccaactgc gaacgatgaa cggtcgacgt tttcgcttgt tctatccggg cgagataagc
    12661 agagcgagtc tcgcgcgaac gttcaatgat tcgatttgtt acgcataaca attgtggatt
    12721 cataaaggct cctgaaattg agttgtcaga gcaggatgat tcacaacgcg tttcattcag
    12781 aggatttatg agtgaaacgc ctgtaaccgg agctcatagg gcaaaaacgt ttcagtcagt
    12841 gtaataaaaa aagcctcgtg ggtgaatccg cacgaggcct gaaagtgtaa aaattgttct
    12901 acaatctgtg caagatcatg ttactggtaa aataaccata aaggataagc gcagatatta
    12961 ctcaaactca ttccaggaac gaccatcacg ggtaatcatc gccaccgagg caacgggtcc
    13021 ccaggttccg gcctgatacg gtttcggcgc atcattgtcc atcgcccacg cctcagtaat
    13081 ggagtctacc catttccagg cttcttccac ttcatcacga cgtacaaaca gtgcctgaat
    13141 accacgcatg gtttccagca gcagacgttc ataggcatcc gccagatgcg tctgattaaa
    13201 ggtttctgaa tagctcagat ccagcttggt gatttgcagg ttatgtttgt ggtcaaggcc
    13261 aggaacttta ttcagtacct ggatatccac gccttcatca ggttgcagac ggatagtcag
    13321 tttattctgc ggcagatcct gccacgactc tttaaacaga ttcagttcag gtgttttgaa
    13381 atagaccacg acttcagaac atttggtcgg cagacgttta ccagtacgca ggtagaatgg
    13441 cacaccggcc cagcgccagt tatcaatgtc gacgcggatc gccacgaagg tttctgtatt
    13501 gctgctcttg ttcgcgccct cttcttccag atatcccggc acttttttgc cctgggcgaa
    13561 gcccgcagta tattgcccgc gtacggtttt ttcgcgtacg ttggagcggt cgatgcggcg
    13621 cagggacttc agtactttca ctttttcatc gcggatgctg tctgcgctca ggtcagatgg
    13681 cggagacatc gcaatcatgc agagaatttg tagcaggtgg ttctgaatca tgtcacgcat
    13741 ctgaccggct ttatcaaaat agccccagcg cccttcgatc cccacttctt ctgccacggt
    13801 aatctcaaca tgatcaatgg tgcgattgtc ccagttattc acaaacaggg agttagcaaa
    13861 acgcagcgcc aacaggttca gcaccgtttc tttaccaaga tagtggtcga tacggtaaac
    13921 ctggcactcc tcgaagtatt cgccaacctg atcattgatt tcctgcgagg tcgccagcga
    13981 cgtccccagc ggtttctcca tgactacgcg tgccggttta gcattcagtt ttgcctcgcc
    14041 aagccctttg caaattgcgc caaaagtgct gggcggcatg gcaaagtagt taatggtgat
    14101 acgatttttt tgatccagca tcgcgccgag acggttgaat gcagcagtgt cattgacatc
    14161 gagattacaa aaatccagac gtgcactcag ggtgtcccat aaaccttcat caatggtttc
    14221 tttcatgaaa gtttcgagcg cctcgcggac aactttggta tatgccgctt tatcccagtc
    14281 agcacgccct acgccgataa tccgggtgtc cgggttgagc tgaccggctt tttccagttg
    14341 atacagggaa ggcagcaatt tacgacgcgc aaggtcgcct ttcgcgccga aaatgaccag
    14401 gtcacaggcc tgggctgttt gcgttaccgc catgtcattc tccttaagtt aactaacccg
    14461 gtacttaagc cagggtatac ttgtaatttt cttacggtgc actgtactgc ttttacgagc
    14521 ttgcgaaaac tgtaaacgct tatccacccg tgcgattacg ggaaaagcgc gcaaagtgcg
    14581 gcaaaactga taaaaaaatc atcgttttcc attcggtaaa aatctgacac tgatcatgtt
    14641 atgaaaaaaa ataacaactt ttttatctgc ttttgtcatt aacacggcac acaggcgtaa
    14701 tatctgacaa aactgcattt cgatttcttt cagtgcggaa atcgtcatta cccgtgagtc
    14761 tctttacatc atgaatatgc tggaaaaaat ccagtctcag ctggaacatt tgagcaaatc
    14821 agagcgcaaa gttgccgagg tcattctggc ttcgcccgat aacgcgatcc attcgagtat
    14881 tgctgctatg gcactggaag ccaatgttag cgaaccgacg gtgaatcgtt tctgtcgcag
    14941 catggacacg cgcggttttc ctgattttaa acttcatctg gcacagagtc tggcgaatgg
    15001 cactccctat gttaatcgca atgtcaatga agatgacagc gttgaatcat acacagggaa
    15061 aatatttgag tccgcaatgg caacgcttga tcatgtccgt cattcactgg ataaatctgc
    15121 catcaaccgc accgtcgact tgctcactca ggcaaaaaaa atcgcctttt tcggattagg
    15181 ctcttcagcc gccgttgccc acgatgcgat gaataagttc tttcgtttta atgttccggt
    15241 ggtgtactcc gatgatatcg tgctgcaacg catgagttgt atgaattgta gcgacggaga
    15301 cgtggtggtg ctgatttctc acactggaag aacaaaaaat ctggtcgagc tggcgcagct
    15361 ggctcgcgaa aacgacgcaa tggtgattgc cctcacctct gcgggtaccc cgctcgcccg
    15421 ggaagcaacg ctggcaatta ccctcgacgt accggaagat actgacattt atatgcccat
    15481 ggtttctcga cttgcacagc tgaccgtgat agatgtgctg gcgacaggat ttactttgcg
    15541 acgcggtgca aaattcagag ataacttgaa gcgggtcaag gaagcgctga aggaatcgcg
    15601 ttttgataag cagttactta atttaagtga cgatcgctaa aaacgactgt cactgtccta
    15661 atcttatacg acatccgaat gagattaatt tatcgccatc gcggcgttat ttcattcgga
    15721 tttcatgttc aagcaacacc tggttgtttc agtcaacgga gtattacatg tccagaaggc
    15781 ttcgcagaac aaaaatcgtt accacgttag gcccagcaac agatcgcgat aataatcttg
    15841 aaaaagttat cgcggcgggt gccaacgttg tacgtatgaa cttttctcac ggctcgcctg
    15901 aagatcacaa aatgcgcgcg gataaagttc gtgagattgc cgcaaaactg gggcgtcatg
    15961 tggctattct gggtgacctc caggggccca aaatccgtgt atccaccttt aaagaaggca
    16021 aagttttcct caatattggg gataaattcc tgctcgacgc caacctgggt aaaggtgaag
    16081 gcgacaaaga aaaagtcggt atcgactaca aaggcctgcc tgctgacgtc gtgcctggtg
    16141 acatcctgct gctggacgat ggtcgcgtcc agttaaaagt actggaagtt cagggcatga
    16201 aagtgttcac cgaagtcacc gtcggtggtc ccctctccaa caataaaggt atcaacaaac
    16261 ttggcggcgg tttgtcggct gaagcgctga ccgaaaaaga caaagcagac attaagactg
    16321 cggcgttgat tggcgtagat tacctggctg tctccttccc acgctgtggc gaagatctga
    16381 actatgcccg tcgcctggca cgcgatgcag gatgtgatgc gaaaattgtt gccaaggttg
    16441 aacgtgcgga agccgtttgc agccaggatg caatggatga catcatcctc gcctctgacg
    16501 tggtaatggt tgcacgtggc gacctcggtg tggaaattgg cgacccggaa ctggtcggca
    16561 ttcagaaagc gttgatccgt cgtgcgcgtc agctaaaccg agcggtaatc acggcgaccc
    16621 agatgatgga gtcaatgatt actaacccga tgccgacgcg tgcagaagtc atggacgtag
    16681 caaacgccgt tctggatggt actgacgctg tgatgctgtc tgcagaaact gccgcagggc
    16741 agtatccgtc agaaactgtt gcagccatgg cgcgcgtttg cctgggtgcg gaaaaaatcc
    16801 cgagcatcaa cgtttctaaa caccgtctgg acgttcagtt cgacaatgtg gaagaagcta
    16861 ttgccatgtc agcaatgtac gcagctaacc acctgaaagg cgttacggcg attattacca
    16921 tgaccgaatc gggtcgtacc gcgctgatga cctcccgtat cagctctggt ctaccaattt
    16981 tcgccatgtc gcgccatgaa cgtacgctga acctgactgc tctctatcgt ggcgttacgc
    17041 cggtgcactt tgatagcgct aatgacggcg tagcagctgc cagcgaagcg gttaatctgc
    17101 tgcgcgataa aggttacttg atgtctggtg acctggtgat tgtcacccag ggcgacgtga
    17161 tgagtaccgt gggttctact aataccacgc gtattttaac ggtagagtaa tctcacctgc
    17221 cccggccgct gatggccggg gtatttagca acacataaat taggactact tattcaatcc
    17281 aatcataatc acagccccct gtaaaatgaa tacagggata gcctgaatgg agatctaaat
    17341 agcttgcaag acgtcgcata cccgcttcct ctgcaacact atgtccaata actgccatcg
    17401 gtaagccact atctttggag tattgtgtct gcacccatgt tttaattgaa tcgttaatcg
    17461 taatataata atcagcctga tattccatat attgtattgg atcacaaaaa cagcctgccc
    17521 caatgccaac agttctgact actctggctt tatctccata aaattgaatg ccaggaagat
    17581 tatatatttt caaatttttg gcaaaacgtt tggtaatgtt aatggcactg tcagggtcta
    17641 tagcatatac acagtacatc tcatcagaag caacaatatt tgtaacatcc agttctaact
    17701 gctgagccaa agccttagac attccgaacg taggctctcg atccatgaca tcatggcaac
    17761 gaatgattac caaattattt ttatgcaaaa actccttttt ttgctcaatt atttttaaat
    17821 aactgagttc accgcgagta taatccatta tttttctggc ccatgaaaac tcaagatcgt
    17881 caccctccag atcagcataa gaataaaatg taggctcatg tgtaataatg atattgacac
    17941 cttcaaaaac agcctttctt aaaacatcca ttgaggcaat ccagcaggtc ccaattttat
    18001 taataagtgt cgcctcagca ccaaaaataa ttttatcaac agttggttgc gaaacagcaa
    18061 ccattgaatt taaaaaacaa ttaagatccg cggctgtttt aatatgatgc attcaacatc
    18121 cttcacaggt ttcatcagca ataaaataat ttcaacattt ctgggaaacg ttttgcccat
    18181 gcagactcag agtgggtatc acctccccat a
//
LOCUS       DAJTZL010000080        18187 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.080, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000080 DAJTZL010000000
VERSION     DAJTZL010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18187
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.080"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(26..871)
                     /gene="adrA"
                     /locus_tag="OO544_002329"
                     /gene_synonym="dgcC"
                     /pseudo
     CDS             complement(26..871)
                     /gene="adrA"
                     /locus_tag="OO544_002329"
                     /gene_synonym="dgcC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308462.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
     gene            complement(973..1293)
                     /gene="psiF"
                     /locus_tag="OO544_002330"
     CDS             complement(973..1293)
                     /gene="psiF"
                     /locus_tag="OO544_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HCR6767912.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(1412..2827)
                     /gene="phoA"
                     /locus_tag="OO544_002331"
     CDS             complement(1412..2827)
                     /gene="phoA"
                     /locus_tag="OO544_002331"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HCR6767913.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTSDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVNDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKTIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADH
                     AHASQVVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(2928..3188)
                     /gene="iraP"
                     /locus_tag="OO544_002332"
     CDS             complement(2928..3188)
                     /gene="iraP"
                     /locus_tag="OO544_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HCR6767914.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPRQ"
     gene            complement(3370..3573)
                     /locus_tag="OO544_002333"
     CDS             complement(3370..3573)
                     /locus_tag="OO544_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6767915.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVREITKMMNGKLIYSWLKWQCG
                     EFFSLNYKLTKRIYFITFIYNKG"
     gene            3651..4745
                     /gene="ddlA"
                     /locus_tag="OO544_002334"
     CDS             3651..4745
                     /gene="ddlA"
                     /locus_tag="OO544_002334"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCR6767916.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALKRHAADNALKATM"
     gene            complement(4769..4981)
                     /locus_tag="OO544_002335"
     CDS             complement(4769..4981)
                     /locus_tag="OO544_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HCR6767917.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            5241..5549
                     /locus_tag="OO544_002336"
     CDS             5241..5549
                     /locus_tag="OO544_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HCR6767918.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(5608..6702)
                     /gene="yaiW"
                     /locus_tag="OO544_002337"
     CDS             complement(5608..6702)
                     /gene="yaiW"
                     /locus_tag="OO544_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HCR6767919.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLAITFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(6715..7935)
                     /gene="sbmA"
                     /locus_tag="OO544_002338"
     CDS             complement(6715..7935)
                     /gene="sbmA"
                     /locus_tag="OO544_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HCR6767920.1"
                     /translation="MFKSFFPKPGAFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGWLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            8287..9444
                     /gene="ampH"
                     /locus_tag="OO544_002339"
     CDS             8287..9444
                     /gene="ampH"
                     /locus_tag="OO544_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HCR6767921.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(9445..10068)
                     /gene="iprA"
                     /locus_tag="OO544_002340"
     CDS             complement(9445..10068)
                     /gene="iprA"
                     /locus_tag="OO544_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HCR6767922.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
     gene            complement(10156..13044)
                     /locus_tag="OO544_002341"
     CDS             complement(10156..13044)
                     /locus_tag="OO544_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR6767923.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANAATYNTFGYHDDAATIYN
                     WGDSTKIDHDYLTYGGYVYDHAADGYFDTTFSGDTVNGVISTYYLNHDYGTDTGNTLN
                     ISNSIVHGMVTSYQVGYGDYVWTNGSDYTGHDWVDGDIFTLNIANSTIDDDFDAFYFT
                     DTYLDADGKTSKTDYDRLVTAGLGTAVTLDVESNINISNNSHVAGITLVQHDLGNATY
                     NTEGHQWDNNIVVNDSTVTSGSWTEDEQSDRGHFGNSVEPSDYGNGASPADDVALAFI
                     DDDTSDYRMVNNVTFNNSQLLGDVVFDSTWNANFDATGHLIDNSTTAYTHGGWATDDQ
                     NVDHLNLTLNNTKWVGSANIDYDVVVADEAFYDVAPNSLNPYASYSEDGWNRVDNANA
                     FQSGVFDVVLNNGSDWETTKTSLIDTLAINSGSQVNVSADSSLTSDTITLNGGSSMEV
                     NGEVNTDHLTIDTFSTVNFGEDTASAWAYAPLYANTITVTNGGVLDVNTNMNDISSVF
                     ATDTLELTSGNVKDNNGNVYAGVFDIHSNDYILNADLVNDRTNDTSKANYGYGVIAMN
                     SDGHLTVNGNNDINNGDEVDNSSADNVVAATGNYKVRIDNSTGAGAIADYAGKQLIYI
                     DDTKTNATFSAANKADLGAYTYQAEQRGNTVVLQQMELTDYANMALSIPSANTNIWNL
                     EQDTVGTRLTNSRHGLADNGGAWVSYFGGNFNGDNGTINYDQDVNGIMVGVDTKVDGN
                     NAKWIVGAAAGFAKGDMNDRSGQVDQDSQTAYIYSSAHFANNVFVDGSLSYSHFNNDL
                     SATMSNGTYVDGSTNSDAWGFGLKAGYDFKLGDAGYVTPYGSVSGLFQSGDDYQLSND
                     MKVDGQSYDSMRYELGVDAGYTFTYSEDQALTPYFKLAYVYDDSNNDNDVNGDSIDNG
                     TEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGDVDQDWSANVGVKYTW"
     gene            13570..14544
                     /gene="hemB"
                     /locus_tag="OO544_002342"
     CDS             13570..14544
                     /gene="hemB"
                     /locus_tag="OO544_002342"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HCR6767924.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            complement(14650..15501)
                     /gene="tauD"
                     /locus_tag="OO544_002343"
     CDS             complement(14650..15501)
                     /gene="tauD"
                     /locus_tag="OO544_002343"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HCR6767925.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRYQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(15498..16325)
                     /gene="tauC"
                     /locus_tag="OO544_002344"
     CDS             complement(15498..16325)
                     /gene="tauC"
                     /locus_tag="OO544_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HCR6767926.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     VLAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            complement(16322..17089)
                     /gene="tauB"
                     /locus_tag="OO544_002345"
     CDS             complement(16322..17089)
                     /gene="tauB"
                     /locus_tag="OO544_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HCR6767927.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            complement(17102..18064)
                     /gene="tauA"
                     /locus_tag="OO544_002346"
     CDS             complement(17102..18064)
                     /gene="tauA"
                     /locus_tag="OO544_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6767928.1"
                     /translation="MAISSRNTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNT
                     FAKESGASVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFAKSAIDAQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTAELTGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
BASE COUNT         4534 a         4728 c         4395 g         4530 t
ORIGIN      
        1 tatcaataag ttggagtcat tacccgctac cgcgcagggt aatttgtaac tgtcgggtaa
       61 gcgccacaat cgcttcatcg cccacatcat gaccccaggt atcgttgatg ctcttgaaat
      121 ggtcgatatc gataatcagt aacgttgcat cgcgattatg ccgccgacag ttatcaaatt
      181 cattgcgtaa catagtttcc caatgacgtc ggttatacac gcccgtcatg ccgtcgcgag
      241 tactcatgac ctgcaacctg cgtttatgtt ccgccagttt ggttgccgtc tggtagctga
      301 cccagccaaa cagcagagga taaacgacaa taatgggaag ggagagccac cattccagcg
      361 gcgcactatt gaacgacacg gtaatgcccg taagctcgag ggtgacaagg caggaaacca
      421 ccatcaacac cagacccgcg acaaacagac gggggccgcc tgcccccatc aaattcagac
      481 acataatcat caacatagcg gtggaaggca gcacgtttac gcccattacg cctacccaca
      541 ttcccgctaa tactgcatcg gtttttaagt tgtaaatttc ccggctaagc ggatcgacgg
      601 ccctgctcgc tatctgccag gctaaatgcg gccagacgaa cgcccagccg accaacacca
      661 gccaccacca gcccggcggc gggtgcgaaa ccagcgttga agcaatcggt aagaacatgc
      721 cagccaggcc aaccgcacgg ggtagtctga cgcgacgggc gaagcgcagc ccggaccgct
      781 gatgttcgtt ttgaggagtt aaaggaggtt cctccccatg cgccgccgct tttttgaaaa
      841 agttttcatc attcattatt tttgggaaca ttcagaaaca attttcccaa attatagaga
      901 cggggctaaa ggcagaagta tgaaatttcg ggtgagccac tttcgtagct cacccgttag
      961 cactatcagg tatcaggcgg ctttcttgag gcaggcactc ataaacttat tacgatcatc
     1021 acctttcaga gattgttgcg ttgcttgatt attacattcg cgcatctttt gctgctgtgg
     1081 cgtcaaactt ttttcaccag gcgcagactt gctgttcttc aggcaatcac tcatgtaggt
     1141 cttacgagca tcccctttca acgcctgcgc cgtcgcctgc tgattacagg aggtcatacg
     1201 ctgttgttgt ggggttaagg ttctctcggc agcgccgacg gtggttaaaa aaaccagacc
     1261 aaaaagcaag gtaaccagta atgttatttt catagcacca tccctcttca tgttttaacc
     1321 atgagcgttt gcgcccgtga tctgccatta agtctggttg ctaacagcaa aaaaaccacc
     1381 cggcagcgaa aattcactgc cgggcgcggt tttatttcag ccccagagcg gctttcatgg
     1441 tgtagaagag atcggtctgg tcggtcagtc caacaacatt ggcggcatgc ggaccatacg
     1501 ccgcaatacg caactgactg ccggtatgtt cttgtgaatc ctcttcggaa ttcccgtaac
     1561 tcatcaccat cactgcgcca tctttagtat ttagcgcctg agtgaggccc ggcgctttgg
     1621 tatccggcgc aacaacctgg ctggcgtggg cgtgatcagc ggtgactatg accagcgtgt
     1681 taccctcctt tttagcgaat tccagcgccc gttgtacggc ttcatcgaga tcgaccgtct
     1741 cgccaatttg cccacaagga ttcgcagcgt gatcctgttt atcgattgac gcaccttcaa
     1801 cttgcaggaa aaatcctttc tcatttttac tcaacaattc aatggttttg tcggtcatct
     1861 gtgccagggt tggtacactg tcattacgtt gcggattagg cgtacaggtg actgcgggct
     1921 tatcgatatt gccgtggtac gttgctttcg gccctagcca gcgcactggc atattgccgt
     1981 cagcaaacag gccaagcagg ggtttttgct gattcgcttc cgtcaccgaa ttcagtgagg
     2041 cagcatcgtt caccaactga taaccacgcg cctgtgcctg ttcacgcagc gtttttccct
     2101 gccattcacc agcggttgcc gtttcagcaa aggtttttgc gccgccgcca agcgtaacgt
     2161 cggcacgagc gttaagcagc tgttcggtaa tcgatccttt tccgcctttt tccagagcgt
     2221 tacccggaca tttttcactg gtcgcgctcg gaccgtagca tttgcgcgaa gtcacatgtg
     2281 ccaccagcgc agcgggcgtg gcatcctgca actctgcggt agaaacgtta ccggtcgcca
     2341 gacctgcggc ttttgccatt tccagaatcg ttgggtgatc tttttcgtga atatcgacgc
     2401 ccagcgcgcc gttataggtt ttgacaccgg ttgaccaggc ggttgctgat gcagccgagt
     2461 cggtgacgta gtccggtttg ccagtttttt tattcagcgc atagtgagtg tattgcccgg
     2521 taagcggtaa ggcatcgata cctttaaaaa agccgcccgc accttcggca taattacgtg
     2581 cggcagtaat ttccgaatcc cccatcccat cgccaatcag caaaataata ttttttgcag
     2641 gtttatcgct aagagaatca cgcagagcgg cggtctgatc actcgttaag cggcgagcac
     2701 cgccgggtgc agtaatatcg ccctgagcag cccggttttc cagaacaggc atttctggtg
     2761 tccgggcttt tgtcacaggg gtaaacagta acggtaagag tgccagtgca atagtgcttt
     2821 gtttcacttt attttctcca tgtacaaata cattaaaaaa taaaaacaaa gcgactataa
     2881 gtctcggccg tgacaacttt atgacagctg ttgaaaagat taacttttta ctgacgagga
     2941 tgcttcaata acttctttac gtaatcgcgc agcagctccg tatcgtcgtc aggaatgctg
     3001 gcatcgggct ttacctcgta cagcgccccc tctacctgat caatcaaccg ctgttggtca
     3061 ttttgcgcca tattgcgaag cattgcagtg acgataatct ccaaagcttc tacctgcgca
     3121 cacagttctt tcgactcttc ttctttttgg gcaagcttaa ataacaactc agcaatgaga
     3181 tttttcatgt ctgtatttcc ttatccaaag tatggagaag ttaacattat gtttcacaac
     3241 ttcatagtgt ctggaagtat tatttttatg acaaaggaaa tactttgcgc aaatattttt
     3301 tgtttgatta ccagcgaaac gtttctctta tttaaataat tacccacaca aaatataggc
     3361 ttttaattat caacctttat tataaataaa agttatgaaa tatattctct tcgttaactt
     3421 ataattaaga gaaaaaaact caccgcattg ccatttaagc caggaataaa ttagcttccc
     3481 gttcatcatt tttgtgatct cgcgcacatc tttgagataa cgcactttct tcaccagtac
     3541 gttgcacgct ttgtcagcag aagattgcaa caatctttca gaaggccgcg aatcccccgt
     3601 tttgtcgtgg taatgtatgc actttgtgtt agacgggata ggtttttaag atggaaaaac
     3661 tgcgggtagg aatcgttttt ggtggtaaat cagcggaaca tgaagtgtct ctgcaatcgg
     3721 caaaaaacat tgtcgatgcc attgataaaa gtcgcttcga cgttgtgctg ctgggcattg
     3781 ataaacaagg gcaatggcac gtcagcgatg ccagcaatta tctgctaaat gcagacgatc
     3841 ctgcccatat tgcgttgcgc ccttcggcga ccagccttgc gcaggtgcca ggtaaacatg
     3901 agcatcagct tatcgacgcg caaaacggtc agccgttgcc gacggtggat gtcattttcc
     3961 cgattgtcca cggtacgctg ggcgaagatg gttccttgca gggaatgctg cgggtcgcca
     4021 atttaccgtt tgtaggttct gatgttctgg cttcagcagc ctgtatggat aaagatgtca
     4081 ccaaacgtct gctacgcgat gccgggctga acattgcgcc atttattacc ctgacgcgcg
     4141 ctaatcgtca caacatcagt tttgccgaag tggagtctaa actggggtta ccactgtttg
     4201 taaaaccggc taatcagggc tcttctgttg gtgtcagcaa agtaaccagt gaagaacagt
     4261 acgcaattgc cgtcgatctg gcgttcgagt tcgatcataa agtgatcgtt gagcaaggga
     4321 tcaaaggtcg tgagatcgaa tgcgcagttc tgggcaacga caatccgcaa gccagcacct
     4381 gtggcgagat cgtactcacc agcgatttct atgcctacga caccaagtac attgacgaag
     4441 atggcgcgaa agtggtagtt ccggcagcca ttgcaccaga gatcaacgat aagatccggg
     4501 cgattgccgt tcaggcttat cagacgttgg gatgcgcagg catggcgcgt gtagacgtgt
     4561 ttttaacccc agagaacgaa gtggtgatca acgagatcaa cacactgcct ggcttcacca
     4621 acatcagtat gtatccgaag ctgtggcaag ccagcggtct gggttacacc gatctgatca
     4681 cacgtttgat tgagctggcg ctgaagcgtc acgctgcgga taacgcattg aaagccacaa
     4741 tgtaatattt taacgcccga taatattctt attgggcgtt ttcagtttct tcatcgcgac
     4801 gacgaataat caaccccgcc agccagaaac taatcaccca cgtcaccaga ccgacggcat
     4861 aggtctgcca accttttgct tcaaatccca gtaaccccac cacgccatta agaatgaata
     4921 taaggccaat ggcgaacgca tagtagtgcc agtcacggcg gatttttaca ggcagattca
     4981 tggcagctcc gttaacgaaa aacaacatcc ttgcatattc tgtaacaacc gtctgtggtg
     5041 tagacaacat tttcgtaatt gttaataaat ttaacttaag gttgccattt tgtaactcac
     5101 aacgaagatt cacgcttcgg gaatattccc aacatcaatt taccatgatt ctgatattga
     5161 caatctaaat gacctgtcag acttgccata agccgctggt atcacaccag aagagaagca
     5221 gatgacttcc ggaggttttt atggctgatt tcaccctgtc aaaatcgctg tttagcggaa
     5281 aatatcgcaa tgcctcttca acgcctggca acattgccta tgcgttgttt gtgctgtttt
     5341 gcttttgggc tggggcgcaa ttgctgaacc tgttagtgca tgcgcccggc gtctatgagc
     5401 gtttaatgca ggtccaggaa acaggtcgcc cacgggtgga aattggttta ggtgtcggca
     5461 ccattttcgg gctgatcccg tttttagtag gctgcctcat ttttgcagtg gtggcgctat
     5521 ggctgcactg gcgacatcgc cgccagtaag atttacgttg ccagatacgg cctaaaacgc
     5581 cttatctggc atgacattcc tgtttgatca ctgtttcata caacgcgccc gccgttcgtc
     5641 tacgcgcttc gcaaaccagg ccgtagtcag gttgcgggtg attttcgggc tttccagttg
     5701 aatgccaggc aacatttcgc ggggtagtga tttacccgtt ttcgcttcgg caagttgata
     5761 aactttcttg tacagcgccg tctcctcaaa agagaagctg tcacctttct ctaactgacg
     5821 gcgaatttcg ctgtcgctca tccccagttt tcccgccagt ttgcgcgttg ccagttcggt
     5881 tttcccgggt tctttactgt cgtaacgaat taaatcaccg tccagtgcca gcttcacgcc
     5941 gctggcctta ctgactgcgt tctgaaacgc ggcattacgg ctggcgtacc agccagcgtt
     6001 aaaatcagca aagcggtata tcggtgcgct atagctggcg ggataattca gtaaatggta
     6061 agtaccaaac cacaacccgc cgcggcgact gaagacttcc tgacgaactg taccgtccat
     6121 tttccacgga taccctttgg tatgctgttc ggcaaaagca atgctgacct gcatcggccc
     6181 accggtgcgc accggattaa gcgaaccaaa cagcgtctgc cccattggta ccatgctgat
     6241 cagatcgtca aaaatcgcgc tcaattgctt ttctgtacgc accgaatcta accgttcgct
     6301 ataacttttg ccgtttggcg atttgatttt tagcgcggta tgaaccagaa aagcaggaat
     6361 gtgcatccgt tcagcacgac ggtcaatctc ttgccaggcg atcttgctta agcccggaac
     6421 ggccggatct gcctgataat ttgactcctg ctgcgccacc gccagcaccg agcagacgtt
     6481 ttccagcgtc ggtgccagcc cctgactttc aaaggtgatg gcgagatctt tcgcccaggc
     6541 atcgcggtct ttcacgctcg caggcatctt ctgccgcacg acgctcgcta catctatcgc
     6601 tttttcgccc tttttcaacg gttgcggagc ctgactacta caccccgcta aaaccagaac
     6661 cgccaacaat gacagcgaag atagatgatt gacgcgcgac attacttctc ctttttagct
     6721 caaggtatgg gttacttcct gaattttgtc accatccagc tcatgttcaa agctgcgcag
     6781 acgtttgtag atagacatca actcaaccag tgtggtccat gagttaatca ggtactggaa
     6841 agcaccgcga acctgaccaa aaacgttggt aatctgcgtc atcaggccga gcgtaatcgt
     6901 accggcaaca attgacggaa acagcaagaa caaaccgaaa acgttatcga cctgcaaata
     6961 gagaatgcgg gcgatgttga aatacatata gtgaaaatag aggcgaaaat agtttttccg
     7021 tacggcgcta aacagctcgc gtaccgtagg cggcgtcgcg cgcgtggcat cgtcttcacc
     7081 ataaaccagc tctttacggt aggcggcctc tactcgctgg tttttaaact ccagccccgg
     7141 cagtttgatc cctaccactg ccagccatcc ggtccccatc agcgaccaga cgatagcggc
     7201 aatcaccaga ccatacggaa tgtgcccgat aatcggcagc tccggcacat gcgcggagag
     7261 cgttaccagc accggcagga aggcgatcaa cgtcatgatg gcgttgataa aactgacgcc
     7321 catattctcc agcgttgaag caaaacgcat ggtgtcttcc tgcacacgct gtgcggcacc
     7381 ttcgatatga cgcagttgtt gccagttcgc catgtaatat tcgttcatcg ccgtacgcca
     7441 gcggaacaca tagtgactga caaagaagtt gttcagtaca ctgatcacca ccgcgatcag
     7501 cgcaatcccc agaaagacgc ccacttcgcg gtaaaattgt tcgatagtga ctttatgcgg
     7561 cgaacttagc gcggtttgaa tcagatcata gaacggtgca taccaggcgt tgacggcgac
     7621 cccgacttcc accaaaaacc aggtgacgaa gatgatcagt gcagtaccga gtattgacca
     7681 gtattgccaa cggtgcgggc tgtagataaa ccagaaaaat gcaaaaagtc ctacgcaaac
     7741 aatgtagtaa gcgtaaaaaa tcaggaaatc caacgaccag aaacgcgcgg cgctaatcgg
     7801 gatctgcccg gaagcgccgg tgatacgcgc cacccagtct cccccaccgg cttgccagaa
     7861 gataacggca atcaatgccc aaacaaaggc cgagagaaaa aacgctcccg gctttgggaa
     7921 aaaagactta aacatatgca ctcctgctaa cttcttatcg ttttaacaac tgctgtgtat
     7981 taaccgcatg accactacaa agcgtcagcc aaatgaaacc gcccattgtg acccatagca
     8041 aggcgcttag ttcgccagcc tgaccataag aattgtttcg acggatttca caaaccaaag
     8101 attgtttaca taagcggatt aacacgcgca ggatcacaaa tgcaggcatt ggtgagcagg
     8161 aaaaagatcg tgtttacgcg ccgttacgtt tttaacacta ttattgcact aattcgccct
     8221 ttgcaatcca tcaatgagta gtataaatac gctcagttac cttcattcaa tctatggaca
     8281 ccaccgttga aacgtagtct gcttttttct gccgtgctgt gtgcggcgtc attgacctcc
     8341 gtccatgcgg cgcagccgat cactgaaccg gagtttgcct ctgatattgt cgatcgttat
     8401 gccgatcata ttttttacgg cagcggtgca acggggatgg cgctggttgt tatcgacggt
     8461 aaccagcgcg tctttcgtag ttatggcgaa acgcgacctg gtaataacgt tcgcccgcag
     8521 ctggattcgg tcgtgcgtat tgcttccctc accaaactaa tgaccagtga aatgctggtg
     8581 aaattgctcg accaggggac cgtaaagtta aacgatccgc taagtaaata tgctccgcca
     8641 ggcgcacgcg tgccgaccta caatgggacg cccattaccc tagtgaatct ggcaacccat
     8701 accagcgccc tgccccgtga acagcccggt ggcgcggcac atcgtccggt gtttgtctgg
     8761 ccaacgcgcg agcaacgctg gaaatacctt tctacggcga agctgaaagc cgcgccaggc
     8821 agtcaggcgg cgtactctaa ccttgcgttc gacctgctgg cagatgcact ggcgaatgcg
     8881 tccggcaagc cttataccca gttgtttgaa gagcagatta cccgtccatt ggggatgaaa
     8941 gacaccacct acaccccctc accggatcag tgccgccgtt taatggttgc tgagcgcggt
     9001 gccagtccgt gcaataacac gctggcggca attggcagcg gcggcgtcta ttccacgcct
     9061 ggcgatatga tgcgctggat gcagcagtat ttgtcatctg atttttatca gcgcagtaac
     9121 caggcagatc gcatgcagac gttgatttat cagcgcgcgc agtttacgaa agtgattggc
     9181 atggatgtcc ccggcaaagc ggatgcgctt ggtttaggct gggtatatat ggccccgaaa
     9241 gagggtcgtc cggggattat tcagaagaca ggcggtggtg gcggtttcat tacctatatg
     9301 gcgatgatcc cgcagaagaa tattggcgcg tttgtggtgg tcactcgttc gccgttgacg
     9361 cgctttaaaa atatgagcga tggcatcaat gatctggtga ccgagctaag cgggaataaa
     9421 ccgttggtta tccccgcatc ctgattaata ctctgaaggc aaacggttga tagcgaccag
     9481 tttgccttta ttcatttcga tatagccgcc tttacgcaaa gcagcgagaa cttctgcgac
     9541 gacagaacgc gatatgcgtg tacgttgatg gatataattc atcacaccaa tacgtgatcg
     9601 caattcttca ttccagtcaa tcattgataa taatgttgcg cggatttgtt cgtaggaatt
     9661 atgcccaatg agctgcacgt cgcgtaattc cagaattcta ttttgccaag ctaaccagta
     9721 aaaagcgtct cgccagagtt gattttgttc aataagcgta atggtttgtt tggctggtag
     9781 atgatatccc gtacaatttc cttctgatat taatttgtat ggtatatcgt ttttcattaa
     9841 accatcagcc agccccataa tataaggggc ctgggtaata ccgataagta cgttttcttc
     9901 tctacgcaga gagataactc cctctaaaat aacgaaagta tcttcgttat tgacatcact
     9961 ggaaaatata acttgctttt cattattaaa ctcgaagcgc gtaccgtatc tggacaaaca
    10021 tttatcgagc ttaccaaatt cctgaagagg tttaactaca gataacattt gcgcgtcctt
    10081 tgcagtaatg cccgtcaaat ccttgacggg cattatttag attaaattac cagtatttct
    10141 tcggagtgaa gaatattacc aggtatattt aacacccacg ttcgcggacc agtcttgatc
    10201 tacgtcacca ccaccgaggt agttagcatc ggtataggcg ctgaagttct tggtgaagct
    10261 gaactgagta cccagaccaa cacgtaccgc agacccttca gtaccgttat cgatggaatc
    10321 accgttcaca tcgttatcgt tgttagagtc gtcgtagacg taagccagtt tgaagtacgg
    10381 agtcagagcc tgatcttcgc tgtaggtgaa ggtataacct gcatctacac ccagttcata
    10441 acgcatgctg tcgtaagact gaccgtcaac tttcatgtcg ttgctcagct ggtagtcatc
    10501 accagactgg aacagaccag aaacgctgcc gtaaggagtc acgtaaccag catcacccag
    10561 tttgaagtcg taaccggctt tcaagccgaa gccccaagcg tcggagttgg tgctaccgtc
    10621 aacgtaagta ccgttgctca tggttgcaga caggtcgttg ttgaagtgag agtagctcaa
    10681 gctaccatca acaaagacgt tgttcgcgaa gtgagcagaa gagtagatgt aggcagtctg
    10741 gctgtcttga tctacctgac cagaacggtc attcatgtca cctttagcga agcctgcagc
    10801 cgcaccgacg atccacttag cgttgttacc gtcaactttg gtatcaacac cgaccatgat
    10861 gccgttaaca tcctgatcat agttgatggt gccgttgtcg ccgttgaagt taccaccgaa
    10921 gtagcttacc catgcgccgc cgttatcagc caggccatga cgagagttgg tcagacgagt
    10981 accaacggtg tcttgttcca ggttccagat attggtgttc gcagacggga tgctcagcgc
    11041 catgttagcg tagtcggtca gctccatctg ttgcagaaca acggtgttac cgcgctgttc
    11101 agcctgatag gtgtatgcac ccaggtcagc tttgttagca gcagagaagg tcgcgttggt
    11161 tttggtatca tcgatataga tgagctgctt acctgcgtaa tcagcaatcg caccagcgcc
    11221 agtggagttg tcgatacgaa ctttgtagtt accggttgca gctacaacgt tgtctgcaga
    11281 gctgttgtca acttcgtcgc cattgttaat gtcgttgtta ccattaacgg tcaagtgacc
    11341 atcagagttc atcgcaataa caccataacc gtagttagct ttgctggtgt cgttggtacg
    11401 atcgttgacg aggtctgcat tcaggatgta atcattacta tgaatgtcaa acacacctgc
    11461 gtaaacatta ccgttgttat ctttaacgtt gccactggtc agttccagag tatcagttgc
    11521 aaatacgctg gagatgtcat tcatgttggt gtttacatcc agcacaccac cgttagtaac
    11581 ggtaatggta ttggcataca gcggtgcata tgcccaagct gaagcagtat cttcaccgaa
    11641 gttaacggtt gagaaggtat caatggtcag gtgatcggtg ttaacttcac cgttaacttc
    11701 cattgaagaa ccaccgttca gagtgatggt gtcagaagtc agagaagaat ctgcactcac
    11761 gttaacctga gaaccgctgt tgatcgccag ggtatcgatc agggaagttt tcgtggtttc
    11821 ccaatcagaa ccgttgttca gaacaacatc gaatacgccg ctctggaatg cgttagcgtt
    11881 atcaacacga ttccagccat cttcagagta agaagcgtac gggttcaggc tgtttggtgc
    11941 aacgtcgtag aaggcttcgt cagcaacaac aacgtcataa tcaatattcg ctgaaccaac
    12001 ccatttggtg ttgttcagag tcaggttcag gtgatcgacg ttctgatcgt cagttgccca
    12061 accgccatga gtgtaagcag tggtagagtt gtcaatcagg tgacccgtcg cgtcaaagtt
    12121 agcgttccag gtactgtcaa atacaacgtc accaagcaac tgagagttgt tgaacgtaac
    12181 gttgttgacc atacgataat cagaagtgtc atcatcgatg aaggctaacg caacatcatc
    12241 agcaggagat gcgccattgc cgtagtcact tggttcaaca gaattgccga aatgaccacg
    12301 atctgattgt tcgtcttcag tccatgaacc agaagttaca gtagagtcat taacaacgat
    12361 gttgttgtcc cactgatgac cttcggtatt gtaggtcgca ttaccgaggt cgtgttgaac
    12421 caaagtaata cctgcaacat gggagttgtt gctgatgttg atgttacttt caacatccag
    12481 agtcacagcg gtaccaagac cagcggttac caaacgatca taatctgtct tagatgtttt
    12541 accgtctgca tccagataag tatcggtgaa gtagaaggcg tcaaaatcat cgtcaatagt
    12601 agaattagcg atgttcagag tgaagatgtc gccatcaacc cagtcgtggc cagtataatc
    12661 gctaccgttg gtccagacgt agtcgccata accaacctgg taagatgtaa ccataccatg
    12721 aacaattgag ttggagatgt tcagtgtatt accggtatct gtgccataat cgtggttcag
    12781 ataataagta gaaattacac catttacagt gtcgccgctg aaagtggtat caaagtaacc
    12841 atcagctgcg tggtcatata cataaccacc gtaagtcaga taatcgtgat caattttagt
    12901 gctgtcaccc caattgtaga tggtggctgc gtcatcgtga tacccgaagg tgttataagt
    12961 agctgcatta gcctgagagg tgatagccag agtgcaagcc aatgctaatt gtgatactac
    13021 aagtttcttt ttccaggagt gcatttgtca tccctcctca gggacgtaat aagttgatcc
    13081 atcaattatc aatgcataga aaacatatta tgtttcgcgc taacgattat gctgcgttac
    13141 agacgaatgg ttcaattatt ctttccccta agtccgaatg tggacatata taatttatgg
    13201 agaaaaacac gcatcaaatt gataaaagga cgatatttca acatgttgca gataatgtta
    13261 tttttgatac atacgcgtta taaatatcaa aaacacaaac cagataaata tcttatcaat
    13321 atacaacaaa gtttgaatta ttcaatttat agcagcacaa ccaaacgcca aaaaaaataa
    13381 taaccaagag ctataaataa agattaaaaa tattttcagt gaatatgaaa ttattgctat
    13441 ttctattaca aagatgtcct ggtctctctt tcattctctt cttgtcaaca tcacgacaac
    13501 tttcgtaaaa cactcctgcc ccgcttcagg tatactgtgc accttaattc cacaaacatc
    13561 aggcagacca tgacagactt aatccaacgc cctcgtcgcc tacgcaaatc tcctgcgctg
    13621 cgcgctatgt ttgaagagac aacacttagc cttaacgacc tggtgttgcc gatctttgtt
    13681 gaagaagaaa ttgacgacta caaagccgtt gaagccatgc caggcgtgat gcgcattcca
    13741 gagaaacatc tggcacgcga aattgaacgc atcgccaacg ccggtatccg ttccgtaatg
    13801 acttttggca tctctcacca taccgatgaa accggcagcg acgcctggcg ggaagatgga
    13861 ctggtggcgc gtatgtcgcg catctgcaag cagactgtgc cagaaatgat cgtcatgtca
    13921 gacacttgtt tctgtgaata cacttctcac ggtcactgcg gtgtgctgtg cgagcatggc
    13981 gtcgacaacg acgcgactct ggaaaattta ggcaagcaag ccgtggttgc agctgctgca
    14041 ggcgcagact tcatcgcccc ttccgccgcg atggacggcc aggtacaggc gattcgtcag
    14101 gcgctggacg ctgcaggctt taaagatacg gcgattatgt cgtattcgac caagttcgcc
    14161 tcttcctttt atggcccgtt ccgtgaagct gccggaagcg cattaaaagg cgaccgcaaa
    14221 agctatcaga tgaacccaat gaaccgtcgc gaggcgattc gtgaatcact gctggatgaa
    14281 gcccagggcg cagactgcct gatggttaaa cctgctggag cgtacctcga catcgtgcgt
    14341 gagctgcgtg aacgtactga attgccgatt ggcgcgtatc aggtgagcgg tgagtacgcg
    14401 atgattaagt tcgccgcgct ggcgggtgct atagatgaag agaaagtcgt gctcgaaagc
    14461 ttaggttcaa ttaagcgtgc gggtgcggat ctgattttca gctactttgc gctggatttg
    14521 gctgagaaga agattctgcg ttaatttttt tcctctcaga aagggggagt taacctgttg
    14581 aaagtacggt gaacgccccc tcaccctaac cctctcccca agggggcgag ggaaccgtcc
    14641 gcactcgtat caccccgccc gataaaacgg tttatcccca aggatcgtcg cccgatgcat
    14701 aatccgccgc tgtggcaggt aatcggcatt ggcatagtgc tgagtcacgc ggttatccca
    14761 aatcgcaata tcattcggtt gccagcgcca acgcacctga aactccggtt tagtgatatg
    14821 ggcgaataaa aagcctaaca aggcttcgct ctctttctcg ctgacatcga caattcgcgt
    14881 agtaaagcct tcattcacaa acagcgcctg tttaccgctc accggatgcg ttcgcaccac
    14941 cggatgtagc aacggaggat ttttcgcgac cgcctcgcgc cagcgttgat gttcctcctc
    15001 ggttttgcgg tatttgtatt ccgggaacga tttacggaaa tcatgctccg cacgcagccc
    15061 gctcagcagc tggcggaagg gaacagagag cgcctcatag gccgcaatac cgctggtcca
    15121 gagcgtatca ccgccggtcg aaggtaactc tttagctgcc agaatcgccc ctgcgggtgg
    15181 cgtttcaata aatgtcacat cggtatgcca gttgtcgtta tccggcggat tatcgttatg
    15241 ggtatccagc acgatgatct cgtcaacccc ttcggcatga gggtaaacag ggtgaatatg
    15301 cagttcgcca aaacgctggg ccagcgcgcg ttgctgctgc ggcgtaatag cttgatcgcg
    15361 cagaaatacc acctgatagc gcagcaccgc atggtaaagc tgttcaaact gattatcgct
    15421 taacgggcgc gtcaggtcgg cacccgaaat ttgtgcgccg atatacggcc ccagcggggt
    15481 aatactcaga cgttcactca ttgtacttct ccatgccagg gcgtcagacg gcgctgtaac
    15541 gcgcgcagac ccagttctaa aagaaaggcg ataatcgcaa tcaccgcgat ccccgccagt
    15601 accacgtcag tcgcgagaaa ttcacccgct gactgaacca taaatcctaa accgcgtgtc
    15661 gcggcaatca gctccgccgc taccagcgta gaccagccca cacccagacc aatgcgtaat
    15721 ccggtgagga tttccggcag cgcgccgggc aaaatgacaa accacagcac ctgcgcacgg
    15781 ctggcaccca gcgactgggc ggcacgaatg cgaacctgct gcgcgctttt cacccccgcc
    15841 agcaccgaca tcgccaccgg tgcaaaaatc gctaaataga tcaataagat cttcgaggtt
    15901 tcaccaatac caaaccagat caccatcagc ggcaaataag ccagcggcgg caccggacga
    15961 taaagttcga ttaccggatc cagaatgccg cgcaccgtcg ggcttagccc catggcgatc
    16021 ccgaccggaa taccgatcac caccgccgcg agtagcgcca gcacaatgcg cgtcaggctg
    16081 gctgccagat gctgccacag cgtggcgtcc ataaatccct gcggtccggc aatggtgagc
    16141 agttttgcca gtacttgttg cggcggcggc aaaaacagcg ggctaatcag ttgcagcgcc
    16201 gccaccgtcc accatacagt gagtaaaacc gctaacgtgc caatgcttaa ggtcacctga
    16261 cgcgagagcg gccagcgcca tttcagccgc cgcgaatgca gtttttcatt aatgagcaca
    16321 ctcatgagaa cgcctcccgt tgctcaaata cgcggcttaa aacatattcg cgcatggcga
    16381 tgaattgtgg atcggacttg atgctgcggc tcgactctcc cgcaacaaag cggcgagcaa
    16441 agttgagcgg cagccgctcc agcacacggc cagggccgga tgaaagcaga accagttcag
    16501 tcgccataaa caccgcttct tctatatcgt gggtaatcaa cagcacctgc ttgcccgtct
    16561 cctgccagag tttcagcagc agagtttgca tctggtcgcg ggtgaaggcg tccagcgcac
    16621 caaacggttc gtcgagtaat aacagctggg gattcgccgc cagcgcgcgg gcaatcccca
    16681 cccgctgacg ttgaccaccg gaaagctgcc agatatagcg tttttctgcg ccttccagcc
    16741 ccactttttt cagcatctgg tgcgcgattt ccagtcgctg cattttctct atacctgcca
    16801 attgcaggcc gaacgccacg ttgtcctgta cattgcgcca cggtagtagc ccttcattct
    16861 gaaaaaccac cccgcgctct gctcccggtc cctcaatacg cttacccgcc agttgaatgc
    16921 tgccatgctg ataaggcaca aaaccggcaa tcagattcag cagggtggtt ttaccgcagc
    16981 cggacggccc cagcaccacc agtagctcgc cgctttccag cgtcaggttg atatcttcca
    17041 gtgccggttt gccgccataa tcggcataga gatgagagat ttgcagcatc tgcgcctcct
    17101 tttattgcac gaagcgcgag gtaacgtact ggctgtaatc attcgctaca gccgggacct
    17161 tgccctgctc tttcaaaaac tgcgcggtgt cgatgattgc tttgttcacc ggtccggtca
    17221 gttctgccgt ttgttgctgc ggcgtcagat aggtattccc cttcaccagc cccggaacgt
    17281 caccttccgg cacgccgctt aaacgcgcca gtttgctgat gttttccggc tgtttcagcc
    17341 acgcgtctgg gttagcaatg tacggttgct gagcatcgat ggcgctttta gcgaacgctt
    17401 tcacgacctc aggatgtttc tcggcaaaat ctttgcgcac cacccagacg tccagcgttg
    17461 gcgcacccca ctgcccgacc tgttcagaat cggtcaacac cttgccgtct ttttccaggg
    17521 cgttaaccgc cggtgcccag acataagcac catcaatatc accccgctgc catgcggcga
    17581 taatcgcggg cggctgcagg ttcacaatct ccacttgccc tggtttaatc ccccagtgtt
    17641 tcagcgccgc cagcaggctg tagtgggtgg tggagataaa cggtacggca atgcgtttgc
    17701 caatcaaatc ttcgggtttg ctgatagttt tctttaccac cagcgcttcg gagttaccca
    17761 gttttgacgc cagcaagaag acttcaatcg gcacctgttg gctggctgca accgctaatg
    17821 ggctggaacc gaggttgccg atttgcacgt cgccagaagc cagcgcccgc acgatgctgg
    17881 ctccgctgtc aaacttacgc cagtccacgc tcgccccgct ctctttagca aaggtgttgt
    17941 cggcctgagc cactttcgcc ggttcggctg aggtttgata cgccacggtg acgttcaccg
    18001 cctgtgcctg aaaagcgatg aatgccagtg cggcaagaag tgtgttacgc gatgaaattg
    18061 ccatgattgt ctgctcccct gtcttgttat gggagcagta ttcaggaata aaaacattca
    18121 ttaaaagaat tagtcgttat cgcacagatg attttattct tagcaaaaaa acggcaaagg
    18181 aaagatt
//
LOCUS       DAJTZL010000081        17961 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.081, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000081 DAJTZL010000000
VERSION     DAJTZL010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17961
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.081"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(231..1205)
                     /gene="cysB"
                     /locus_tag="OO544_002347"
     CDS             complement(231..1205)
                     /gene="cysB"
                     /locus_tag="OO544_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HCR6767929.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFTIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            complement(1415..4012)
                     /gene="topA"
                     /locus_tag="OO544_002348"
     CDS             complement(1415..4012)
                     /gene="topA"
                     /locus_tag="OO544_002348"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HCR6767930.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTIGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVNKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKIGEIVTDRLEENFRELMNYDFTAQMENSLDLVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            4392..4643
                     /locus_tag="OO544_002349"
     CDS             4392..4643
                     /locus_tag="OO544_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HCR6767931.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FIGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            complement(4679..5728)
                     /gene="sohB"
                     /locus_tag="OO544_002350"
     CDS             complement(4679..5728)
                     /gene="sohB"
                     /locus_tag="OO544_002350"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HCR6767932.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            5948..6706
                     /locus_tag="OO544_002351"
     CDS             5948..6706
                     /locus_tag="OO544_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HCR6767933.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            6703..7293
                     /gene="cobO"
                     /locus_tag="OO544_002352"
     CDS             6703..7293
                     /gene="cobO"
                     /locus_tag="OO544_002352"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HCR6767934.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIKHAFDAGVKAQIGIDY"
     gene            complement(7333..8205)
                     /gene="rluB"
                     /locus_tag="OO544_002353"
     CDS             complement(7333..8205)
                     /gene="rluB"
                     /locus_tag="OO544_002353"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HCR6767935.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNG"
     gene            complement(8418..9532)
                     /locus_tag="OO544_002354"
                     /pseudo
     CDS             complement(8418..9532)
                     /locus_tag="OO544_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309867.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
     gene            complement(9560..10180)
                     /locus_tag="OO544_002355"
     CDS             complement(9560..10180)
                     /locus_tag="OO544_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HCR6767936.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            complement(10177..11058)
                     /gene="yciV"
                     /locus_tag="OO544_002356"
     CDS             complement(10177..11058)
                     /gene="yciV"
                     /locus_tag="OO544_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exoribonuclease"
                     /protein_id="HCR6767937.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEVLVHRAVEMRVGTLAITD
                     HDNTAAIVPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQLIAERLEKAQIPGALEGARRLAQGGAVTRGHFARFLVGCGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLAALARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            11196..11240
                     /gene="trpL"
                     /locus_tag="OO544_002357"
     CDS             11196..11240
                     /gene="trpL"
                     /locus_tag="OO544_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HCR6767938.1"
                     /translation="MKAIFVLKAWWRTS"
     misc_feature    11217..11310
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            11332..12894
                     /gene="trpE"
                     /locus_tag="OO544_002358"
     CDS             11332..12894
                     /gene="trpE"
                     /locus_tag="OO544_002358"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HCR6767939.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGNRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQALSGNGEALLALLDNALPAGVENEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKNTRIQASLFAPNEEEKQRLTARLNDLRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSSYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSIPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     gene            12894..14489
                     /gene="trpD"
                     /locus_tag="OO544_002359"
     CDS             12894..14489
                     /gene="trpD"
                     /locus_tag="OO544_002359"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HCR6767940.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPILMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            14490..15851
                     /gene="trpCF"
                     /locus_tag="OO544_002360"
     CDS             14490..15851
                     /gene="trpCF"
                     /locus_tag="OO544_002360"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HCR6767941.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIASIYKHYASAISVLTDEKYFQGSFDFLP
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLILVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPARELQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            15863..17056
                     /gene="trpB"
                     /locus_tag="OO544_002361"
     CDS             15863..17056
                     /gene="trpB"
                     /locus_tag="OO544_002361"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HCR6767942.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPEKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            17056..17862
                     /gene="trpA"
                     /locus_tag="OO544_002362"
     CDS             17056..17862
                     /gene="trpA"
                     /locus_tag="OO544_002362"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HCR6767943.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPIEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISTPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKVATRS"
BASE COUNT         4317 a         4750 c         4488 g         4406 t
ORIGIN      
        1 aatccagcga atttacgaat cgtctccagt gaataattca atcttttgac ctgaaataga
       61 tatttgaaaa ctggagtttg cgaggcgggt aattagacac tactttacag gtaatttacc
      121 ctataaatat gagggataaa aatattgtgc acggtataga aataagtaac aaaagagaat
      181 atattccggc accttcgcta cataaaaggt gccgaaaata acgcaagaaa ttatttttcc
      241 ggcagtttta tatctttaaa catgacctca atttcttcat tagagcgcaa tgcgacagcc
      301 gcatcaacga catcacgcgt taaatgcggt gcaaaacgct gaatgaaatc atacatataa
      361 ctgcgcaaga aagtactacg gcgaaaacca attttggttg tactgtggct gaagatatcg
      421 tgagcatcaa cacgcacaag gtcgggatcg gcgaccggat ccaccgccat gctggcaatg
      481 acccctaccc ccagccctaa ccggacgtaa gttttaatga cgtcagcatc cgttgccgtg
      541 aagacgatac gcggcgttaa ccctgcgcgg ttaaaggcag tatccagttc tgaacgtccg
      601 gtaaagccga aggtatatgt caccaacgga tattgcgcca gttcttcaat ggtaatggct
      661 tttttgcctg ccagcgggtg atccggagtg actacaatag cccgattcca gtggtagcac
      721 ggtaacatca ctaaatcttc atacagatgc agcgcttctg tggcgatagt gaaatcagca
      781 ttgcctttag agacggcatc agcaatttgt gtcggcgagc cctggtgcat atgcaaagaa
      841 acgcggggat aacgctcaat aaagcctttg ataacgtttg gtaacgcgta gcgtgcctgg
      901 gtatgcgtgg tggcgatata cagtgagcct ttatccggcc aggtgtgctc tccggcaacc
      961 gattttatgg catcgacttt cgacaggact tcgcgagcga tacgaattat ttcttgccct
     1021 gctggcgtta cctgcgtcag gtgcttgccg cttcgggaaa aaatttgaat gcctagctcg
     1081 tcttccagca ttctgacttg tttactgatc ccgggttgtg atgtgtaaag tccttccgct
     1141 gttgatgaga cattcaggtt atgattgacc acctcaacaa tatagcgaag ttgttgtaat
     1201 ttcatgttaa accatccact tagacgccat cagaacatcg ttttttcgtc acacctggcg
     1261 ggtgactaat acgatttaat aataaaaaag gtgctaacta taaccactat atcatttata
     1321 gccttagtgt ataggtgtga agaaaaaata atatgcaata aaaaagggcc gctttcgcga
     1381 ccctttgttt ataaaaacct gacagaatta aaggttattt ttttccttca acccatttgc
     1441 catcaacata aaatgctgac cagccagtcg cctttccgtc tttttccgaa gagacatatt
     1501 gctgtttggt tttacggcta aagcgaacca tggtcttatt accttccgga tcctgctgtg
     1561 gcgcatcggc cagataacgc agtttttccg gcagacggtc gcggaagcga taaagctctt
     1621 ccaccagtgg cgcacgcgtt tcacgcgatt tcgggaaagt gttggcagcc aggaaaacac
     1681 ccgcagcgcc gtcacgcagc acgaaataag catctgattt ttcgcacggc agctcaggta
     1741 atggcaccgg atcttctttc ggtggtgcca cttcgccgtt acgtaaaatc ttacgtgtgt
     1801 ttttacactc ttcgttggtg caggccatgt atttaccgaa acgccccatt ttcaggtgca
     1861 tttcagagcc acatttttca cactcaacga tcgggccgtc ataaccttta atgcggaatt
     1921 cgccctcttc gatctcgtaa ccgtcgcagg ttgggttatt accacagaca tgcaacttac
     1981 gtttcggatc gatgagatag ctgtccatcg ccgtgccgca tttcgggcaa cgacgttttg
     2041 cgcgcagcgc gttggtttca gcgtcttcgc cttccagtac gttcagcact tcgttttccg
     2101 gcaccaggtt aatggtggtt ttgcaacgct ctttcggcgg cagcgcataa ccagaacagc
     2161 caaggaatac cccggtgctc gctgtgcgaa tccccatttt gcgaccacaa gtcgggcagt
     2221 caatgctggt cagaaccatc tggttcgggc gcataccacc ctcttccgga tctttttcag
     2281 ctttatctaa ctgctgggtg aaatccgaga agaagtgatc cagtacagct ttccactctg
     2341 cttcgtgatt tgccaccagg tcgaggctgt tttccatctg tgcggtgaag tcgtagttca
     2401 ttaactcgcg gaaattttct tcaaggcgat cggtgacgat ttcgccaatt ttttccgcat
     2461 agaaacgacg attttctact cgcacgtagc cacgatcctg aatggtcgaa atgatcgacg
     2521 cataggtaga cggacgaccg ataccgcgtt tttccagctc tttaaccagc gatgcttcac
     2581 tgaaacgggc tggcggcttg gtaaagtgct gggctggtgt aagttcaacg agcgtcagag
     2641 catcgccttt attaactgct ggtaagatgc gatcttcatc gcctttacgc aacgcaggca
     2701 tcacttttgt ccagccatca aaacgcaaaa tacgaccgcg tgctttcagg cggaaatcgc
     2761 ccgcaccaat ggtcagcgtc gtggagtcat atttcgctgg ggtcatctgg caggctacga
     2821 actgacgcca gattaactgg tacagtttct gcgcatctgc ttccatatcc ttcagcgatt
     2881 ccgccatcac attgacgtca gaaggacgaa tcgcttcgtg cgcttcctgt gagttttctt
     2941 tgctggcgta ctgattcgga ctttccggca gatatttctt accaaaatta tcgctgatat
     3001 aaccacgaac catatttacc gcgtcctgac tcaggttagt ggagtcggta cgcatgtaag
     3061 tgatatagcc tgcttcatac aaacgctgcg ccatcatcat ggtttttttc acgccaaatc
     3121 caagacgggt gctggcagct tgttgcagcg tagaggtaat aaaaggagcg ccaggtttac
     3181 tggttgtcgg tttgtcttca cgttccagca cgctgtagcg cgctttttcc agcagactta
     3241 ccgcagcctg agtttgttct ttgttgaccg gacggaacgg tttgtcgttc tgatgagtca
     3301 cctgtaacgc caacgcttca ccagatggcg tggtcgtgct ggcatcgact tcccagaact
     3361 cttccggcac gaacgcttta atttcacgct cacgctcgac caccaggcga accgccaccg
     3421 actgcacacg accggcagac agaccacgag cgatcttttt ccatagcagc ggcgaaacca
     3481 tataccccac cacgcggtcc ataaagcgac gcgcctgctg ggcattaaca cgatcaatat
     3541 tcagctcacc cggtttgtta aatgcctggc ggatcgcgtt tttagtaatt tcgttaaaca
     3601 ccactcggct atagcgcgca tcatcacccc caatcacttc ccgcaggtgc catgcaatgg
     3661 cttccccttc gcggtcaagg tcggttgcga gatagatgtg gtcggctttt tcagccagtt
     3721 gtttcagttc agagacgacc ttctctttac caggcaacac ttcatagtgc gcctcccaat
     3781 tgtgccacgg gtcaaccccc atacggttga cgagagcgcc acgttcatcc tttttaggct
     3841 ttttagccgt cttggtggag gtagagtcgg cactcttttt ggcagctgag ccactggtcg
     3901 gcaaatcgcg gatgtgaccg acgctggatt tcaccacgta gtcactaccc agatacttgt
     3961 tgatcgtttt ggcttttgcc ggggactcaa cgatgacaag agctttaccc atattcacct
     4021 tacctaattt gattcttcca ggaatgcgtc gcacgttgat aaaccttcca ctggcaacga
     4081 gttaccgata tggactcgtt ctctgcggat atcaacccct tgtcgtgact ctgttcacga
     4141 aaattcaggt gactgagtta acaggtcttg gcctacaggc gctatagcac gacggaattt
     4201 tggtcgaatg tcaagcaaat ctattgccag attgacgaaa gcgtcacact gtacctgata
     4261 aaatccgata cgcaacttta ttagcatgca aaatccaaag ttgccagtaa ggcgctcttg
     4321 ccaccagcgt ggtatatttg tctgcaagcc atgcctggcg tagactaatg tccttgttta
     4381 aggagtaaat aatgaacaaa gagacacaac ccatcgaccg cgaaacgctg ttgaaagagg
     4441 ccaacaaaat cattcgtgag catgaggata cgctcgcagg gattgaagcc accggcgtca
     4501 cgcagcgtaa tggcgtgctg gtatttatcg gcgactactt tttagatgaa caaggattgc
     4561 caacagccaa aagcaccgcg gtgtttaata tgttcaaaca cctggcccat gtgctttcag
     4621 aaaaatatca tctggttgat taaagcaaaa cgcgaggtct tagcctcgcg tttgtctttt
     4681 acatcaatgg cttttgaccc cgctgccacc agcgtagcaa caatcgatcg gcgctctctg
     4741 ccgcgctgcc ggtgaatcgg tcaatgagtc gtttacgctg catatagcgt acattaacca
     4801 cttcacggcc ttccatcagg ctaagaataa cttcatcact ggtgttgatt tcatcaacca
     4861 ggcctttctc taccgcctgt tgtccgtacc agtgttcacc cgttgccacc tgttcaatat
     4921 ccagagacgg acgcatacgc ttcacaaaat ctttaaataa ctgatgcgtt tcgttcagct
     4981 cttcgcggaa tttctcccgc ccttcttcgg tattttcacc cagcaaggtc agcgtgcgct
     5041 tatactgccc ggcggtgtgc agttcgatat caatatcttt gcttttcagg aagcggttaa
     5101 agttgggcat ttgcgccacc accccaatgg aacccacaat agcaaacggt gcggaaacaa
     5161 ttttgtccgc cacacaggcc atcatgtaac cgccgctggc agcgactttg tctaccgtaa
     5221 cagttaaagg aatgttttta tcacgcagac gctgcagctg cgaagccgcc aacccgtaac
     5281 catgcaccat gccaccaggg ctttccagac ggagcacaac ctgatcctgc ggtttgaatg
     5341 ctgcgagtac cgccgttatc tcttcacgta gcgagttcac ttcatgggcg tccatgctgc
     5401 ctttaaaatc cagcacccag acgcgaggtt tactgtcagt tgccacctcg cccagtttgg
     5461 ctttcgcttt tgctgctttc gcttcttgct tgtgcttttt cttctgcgct ttgtgccact
     5521 gtttttgctg atgtgagtcc atcagcgccg cggccagttc ttctttcatc tccttatact
     5581 gttcgctgag attgttgacc cgtaactcgc cacgctggcg tttattacgt tgagcaacat
     5641 tgacaataat ggcggcaatc gccgcaatcg ctagcacaac ggtaacgatt ttcgccaaaa
     5701 acaaaccata ttcagacaac aattccacga gtccccacct tggttaaaca acgctttggc
     5761 tcacagttta caacaggcat gttaagccgt ctcgcagaga tgttattgca gcgaaagtat
     5821 gacaaagtat ccattttgcg gtgttatcgc cccaggctga ttgaaatgcc cttctgtttc
     5881 aggcataaag ccccaaagtc ataaagtaca ctggcagcgc gacagacaac gtcgttgagg
     5941 agtagccatg cattaccagc caaaacaaga tttactcaat gatcgcatta tcctggtgac
     6001 gggagccagc gatgggattg gtcgtgaagc cgcgatgacg tatgcacgct atggtgcgac
     6061 agtgattctg ttgggccgta atgaagaaaa attacgtcag gtagccagcc acataaacga
     6121 agaaactggg cgtcagccac agtggtttat tctcgatttg ctgacctgca cgtccgaaaa
     6181 ttgccaacaa ctggcacagc gcattgtcgt taattatccg cgtctggatg gtgttttgca
     6241 taatgccgga ttgctcggcg atgtttgccc aatgagcgaa caaaatccgc aggtctggca
     6301 ggacgtcatg caggtcaacg ttaatgccac ctttatgctc acccaggcac tgcttccttt
     6361 attactcaaa tcggacgccg gttcactggt ctttacttca tcaagcgttg ggcgtcaggg
     6421 acgagccaac tggggtgcat atgcagcgtc gaaatttgcc accgaaggga tgatgcaggt
     6481 actggccgat gaatatcagc agcgcctgcg cgtcaactgc attaacccag gcggtacgcg
     6541 caccgcaatg cgcgcaagcg ccttcccgac cgaagatcca cagaaactta aaacacccgc
     6601 tgatatcatg ccgctctacc tctggctgat gggcgatgac agccgccgta aaaccggcat
     6661 gacctttgac gcccaaccgg gccgtaaacc aggaatttcc caatgagtga tgaacgctac
     6721 caacagcgtc agcagcgagt gaaagaaaaa gtagatgctc gtgtggccca ggcccaggat
     6781 gaacgcggta ttatcatcgt ctttaccggc aatggaaaag gcaaaaccac cgcggcattt
     6841 ggtacggcaa cacgcgcagt tggtcacgga aaaaaagtgg gcgtcgtgca gtttattaaa
     6901 ggcacctggc ctaatggcga acgcaatctg ctggagccac atggcgttga gtttcaggtg
     6961 atggcaacgg gctttacctg ggatacacaa aaccgcgagt ctgataccgc cgcctgccgc
     7021 gaagtctggc aacatgcaaa gcggatgctt gctgattcct cactggatat ggttttgctt
     7081 gatgaactga cgtatatggt ggcgtatgac tatttgccac tggaagaagt ggtgcaggcg
     7141 ttaaatgaac gtccacatca acagacggtg attatcacgg gtcgaggctg tcatcgggat
     7201 attcttgaac tggcggatac ggtaagtgag ctgcgcccca tcaaacatgc gtttgatgcc
     7261 ggtgttaagg cgcaaatagg gattgattat taatctgtaa aaatgccgga tatgacgtcc
     7321 ggcatacttt gattaaccgt tattacgccc gccagaacga cgaccgccgc tcacctgact
     7381 gtgacgtttc accgcacgac gaatctgatt agccttcatg cgacgacggt ctttttctac
     7441 agcgacttta gagctggttt ctggcggaag ctccaccagt tcgcgcagat agttagtctg
     7501 ggcgaggtcc agctccgtcc agccaccgcg tggcagacct tttggcagtg ggatatcacc
     7561 gtaacgaaca cggatcaggc ggctcacctg cacaccaacc gcttcccaca gacgacgaac
     7621 ttcacggtta cggccttcgg tcagagtcac gttgtaccac tggttgatcc cttcgccgcc
     7681 gctgaacttg atggttttaa aagctgccgg accatcttcc agttgcacgc cacgactcaa
     7741 atcacgcagt ttcgcgtcgt caacctgacc aaatacacgc acggcgtatt cacgttcaac
     7801 ttcacggctt ggatgcatta aacggtttgc cagttcacca tcggtggtga acagcagcag
     7861 accacaggtg ttaacgtcca gacgacctac ggcaatccag cgtgcgccac gcagctttgg
     7921 cagacggtca aacaccgtcg gacgcccttc cggatcgtta cgggtacaca actcaccttc
     7981 cggtttgtaa taggccagaa cgcgacaaat ttgttcagcg gactcacgta ccgaaatcag
     8041 atgaccatcg atacgaattt tcagcccagg ggtaacttca acgcgatcgc cgagtttagc
     8101 aattttgcca tcaacactca cacgaccggc ttcaataata gattcgattt cacggcggga
     8161 gccgtggcca gcgcgggcca gcactttctg taatttttcg ctcatagagc ttccttttgg
     8221 tgtcgccttc ccaggcgtct gatatgcttt tgtgcaacag tgagtcagaa cttacatctg
     8281 actcactgat agtaaagtct gtttgaactc ttcaggcctt aagtaaacga ctcgacgttt
     8341 taactggcct ggctttgagg cgcaacatac tacactatct ttatggttaa agcttgtttc
     8401 gattaacatc agcttaatta ccagccacca ccgcctccgc cacccgcgcc gccaccagca
     8461 ccaccaccgg aataacttcg ccccaacaaa ctggttttgg cccccagact caggtgcatc
     8521 tgtaatgaaa acaaaacgcc actacgggca atttccgggg cgctaacagc cgctgtgagt
     8581 gatggggtga tacgtgctac ccattctctt ccaaggtttg ctgagactgc ccaacctagc
     8641 aacgcgtaat ccagtttccg tgtttcccct ttccgccgac gcccactgtg tgttgctgcc
     8701 tctttacgcg caagatagtt aacgatagct tcaccgtggg cataacgttg ctgcccagtt
     8761 tgtgtaaact ttggcagaaa aatatacccc atgccagtga gataaccggt caggaaaaca
     8821 gcgctaaaat aacccgctgg catataccag taaaagaata catctccgac attgatgtag
     8881 agagaataga aggccacgcc tgaacaaatc agtggtaaac aattcttccg tcctcaagga
     8941 tgcggccatt tcgatatgtg tcgccctctt ctccagtaaa gagatcgatt tcgctaaatg
     9001 gattacccgc gatatcaggg aacttgagtg aaaatcgggt cttatagatt tcaaacggcc
     9061 agtggttacc agtcacgttc cagattaaca gatctgaatc tccctcacgc aggaaagcat
     9121 ttttaacctg ataatgaatt tgataatgat agtcgccttg aggcaagaaa cgttgtttat
     9181 cgcccacgac aatactcatt aacccgatat gccagtcatg atgccagggt tcaggaatac
     9241 catcacgggt gacgccaaca atatgatagt tcagcagtgc agcgtcccca ccaggctgca
     9301 tccagagtcg tggtaaatca cgataaaatc cgtgtttaat ctgctgattt cgcgcctgaa
     9361 aatgaatatt ttctttgatg tcgagcaccc catccggttg cagagtggct tcaacatcaa
     9421 agagcgaaat cccttcttca taagaaggga tctccgtgtt ttctgcataa cttagagatg
     9481 atacaaatag ccctgctatc aacagcaaaa tgcagcgaaa cttccctgcc atagtcttcc
     9541 atccgtggaa cgcaatttgt tataagaaag gcttcacatc acctacgcct tcacgcacca
     9601 cgaccggcgt atcatcggta agatcaataa ccgttgtcgg tttctgcccg agatagccgc
     9661 cgtgaataat caaatctacc tgtttttcca gacggtcttt aatttcttcg ggatcggatt
     9721 cggtaaattc gctgcctggc agcattagcg aagtggaaag catcggttcg cccagtgctt
     9781 caagcaacgc ctgcgcaatc gggttagaag gcacacgcat accgatggtt ttgcgttttt
     9841 cctgcaacag acggcgtggc acttccttcg tccccttcag gatgaaggta tagttgcccg
     9901 gcgtgttgtt tttcattaaa cgaaacgcaa cgttatcaac aaatgaatag gtcgagagtt
     9961 cagaaagatc gcgacacatc agggtaaagt tgtgaccgtc cggcagctgg cgaatacgac
    10021 aaatacgctc catcgcgttt ttatcttcaa ttttacagcc gagcgcatag ccggaatcag
    10081 ttggataaac aatcaccccg cctttacgca cgatctccac cgcctggttg atcagacgtt
    10141 gctgtgggtt atcaggatga atataaaaaa actggctcat aattccctct ctgtggtgtt
    10201 ctgcggctgt tcccatagct gccagacgcc ttcaacgcct gcgggcagcc agagtttacg
    10261 acctagttca atccacgggc atggctgatg aaaatcagat ccttgtgatg cccataaatg
    10321 atgctgacgc gcaagggccg ccagctgggt acgttcattg ggcgattgct ggcactgcgc
    10381 gacttccatc gcgtcaccgt ggtgttcggc aaaatgcgca accaatcttt tcagccattt
    10441 agcactaaga ttgtaccgcc caggatgagc tagcaccgcc ttaccgccag aatgatgaat
    10501 gacatcaata gcttgttcta ttgtacacca ctgtggcgga acgtatccgg ttttcccgcg
    10561 cgccagatat tttttaaaga catccgccat tgaactggct ttgccgcacc ccacaaggaa
    10621 acgcgcaaaa tgaccgcgcg tcaccgcacc gccctgcgcc agtcgtcgcg cgccttccag
    10681 tgcccctggt atttgcgctt tttcaagtct ttcggcaatc agttgcgccc gctgattgcg
    10741 ccgttctgtc tgctgcgcga ggaactcaca cattagcgga tgagtaatat caatattcag
    10801 cccgacaata tgaatttcat gattttccca gaccgtggaa atttccacgc cgggaataag
    10861 attcagcgcc agtcccgaac gtgaaatttc ctctcttgct ggcacgattg cagctgtgtt
    10921 gtcatggtcg gtgatcgcca gggtaccgac gcgcatctcg actgcacggt gcaccaatac
    10981 ttctggcgtc aggcagccat cggaagctgt ggtatggctg tgcaggtcgt aaatcactgc
    11041 gtaattcgtg tcgctcaagg cgcactcccg ttctggataa tgttttttgc gccgacatca
    11101 taacagttct ggcaaatatt ctgaaatcag ctgttgacaa ttaatcatcg aactagttaa
    11161 ctagtgcgca agttcacgta aaaagggtat cgacaatgaa agcaattttc gtactgaaag
    11221 cttggtggcg cacttcctga aacgggcagt gtattcacca tgcgtaaagc aatcagatac
    11281 ccagcccgcc taatgagcgg gctttttttt gaacaaaatt agagaataac aatgcaaaca
    11341 caaaaaccga ctctcgaact gctaacctgc gaaggcgctt atcgcgacaa cccgactgcg
    11401 ctttttcacc agttgtgtgg gaatcgtccg gcaacgctgc tgctggaatc cgcagatatc
    11461 gacagcaaag atgatttaaa aagcctgcta ctggtagaca gtgcgctgcg cattacagca
    11521 ttaggtgaca ctgtcacaat tcaggcactt tccggcaacg gcgaagccct gctggcacta
    11581 ctggataacg ccctgcctgc gggtgtggaa aatgaacaat caccaaactg ccgtgtgctg
    11641 cgcttccccc ctgtcagtcc actgctggat gaagacgccc gcttatgctc cctttcggtt
    11701 tttgacgctt tccgtttatt gcagaatctg ttgaatgtac cgaaggaaga acgagaagca
    11761 atgttcttcg gcggcctgtt ctcttatgac cttgtggcgg gatttgaaga tttaccgcaa
    11821 ctgtcagcgg aaaataactg ccctgatttc tgtttttatc tcgctgaaac gctgatggtg
    11881 attgaccatc agaaaaaaaa cacccgcatt caggccagcc tgtttgctcc gaatgaagaa
    11941 gaaaaacaac gtctcactgc tcgcctgaac gatcttcgcc agcagctgac cgaagccgcg
    12001 ccgccgctgc cggtggtttc cgtgccgcat atgcgttgtg aatgtaatca gagcgatgaa
    12061 gagttcggtg gcgtagtgcg tttgttgcaa aaagcgattc gcgctggaga aattttccag
    12121 gtggtgccat ctcgccgttt ctctctgccc tgcccgtcac cgctggcggc ctattacgtg
    12181 ctgaaaaaga gtaatcccag cccgtacatg ttttttatgc aggataatga tttcaccctg
    12241 tttggcgcgt cgccggaaag ttcgctcaag tatgacgcca ccagccgcca gattgagatc
    12301 tacccgattg ccggaacacg tccacgcggt cgtcgtgccg atggctcgct ggacagagac
    12361 ctcgacagcc gcatcgaact ggaaatgcgt accgatcata aagagctttc tgaacatctg
    12421 atgctggtgg atctcgcccg taatgatctg gcacgcattt gcacccccgg cagcagctac
    12481 gtcgccgatc ttaccaaagt tgaccgttac tcttacgtga tgcacctggt ctcccgcgtg
    12541 gtcggtgagc tgcgccacga tctcgacgcc ctgcacgctt accgcgcctg tatgaatatg
    12601 gggacgttaa gcggtgcgcc gaaagtacgc gccatgcagt taattgccga ggcggaaggt
    12661 cgtcgccgcg gcagctacgg cggcgcggta ggttatttca ccgcgcatgg cgatctcgac
    12721 acctgcattg tgatccgctc ggcgctggtg gaaaacggta tcgccaccgt gcaagccggt
    12781 gctggcgtag tccttgattc tattccgcag tcggaagccg acgaaacccg taataaagcc
    12841 cgcgctgtac tgcgcgctat tgccaccgcg catcatgcac aggagacttt ctgatggctg
    12901 acattctgct gctcgataat atcgactctt ttacgtacaa cctggcagat cagttgcgca
    12961 gcaatgggca taatgtggtg atttaccgca accatattcc ggcgcagacc ttaattgaac
    13021 gcctggcgac gatgagcaat ccgatactga tgctttctcc tggccccggt gtgccgagcg
    13081 aagccggttg tatgccggaa ctcctcaccc gcttgcgtgg caagctgccc attattggca
    13141 tttgcctcgg acatcaggca attgtcgaag cttacggggg ctatgtcggt caggcgggcg
    13201 aaattcttca cggtaaagcg tcgagcattg aacatgacgg tcaggcgatg tttgccggat
    13261 taacaaaccc gctgccggtg gcgcgttatc actcgctggt tggcagtaac attccggccg
    13321 gtttaaccat caacgcccat tttaatggca tggtgatggc agtacgtcac gatgcggatc
    13381 gcgtttgtgg attccagttc catccggaat ccattctcac cacccagggc gctcgcctgc
    13441 tggaacaaac gctggcctgg gcgcagcaga aactagagcc agccaacacg ctgcaaccga
    13501 ttctggaaaa actgtatcag gcgcagacgc ttagccaaca agaaagccac cagctgtttt
    13561 cagcggtggt gcgtggcgag ctgaagccgg aacaactggc ggcggcgctg gtgagcatga
    13621 aaattcgcgg tgagcacccg aacgagatcg ccggggcagc aaccgcgcta ctggaaaacg
    13681 cagcgccgtt cccgcgccca gattatctgt ttgctgatat cgtcggtact ggcggtgacg
    13741 gcagcaacag tatcaatatt tctaccgcca gtgcgtttgt cgccgctgcc tgtgggctga
    13801 aagtggcgaa acacggcaac cgtagcgtct ccagtaaatc tggttcgtcc gatctgctgg
    13861 cggcgttcgg tattaatctt gatatgaacg ccgataaatc gcgccaggcg ctggatgagt
    13921 taggtgtttg tttcctcttt gcgccgaagt atcacaccgg attccgccac gcgatgccgg
    13981 ttcgccagca actgaaaacc cgcaccctgt tcaatgtgct ggggccattg attaacccgg
    14041 cgcatccgcc gctggcgtta attggtgttt atagtccgga actggtgctg ccgattgccg
    14101 aaaccttgcg cgtgctgggg tatcaacgcg cggcggtggt gcacagcggc gggatggatg
    14161 aagtttcatt acacgcgccg acaatcgttg ccgagctgca tgacggcgaa attaagagct
    14221 atcaattgac cgctgaagat tttggcctga ctccctacca ccaggagcaa ctggcaggcg
    14281 gaacaccgga agaaaaccgt gacattttaa cacgcttgtt acaaggtaaa ggcgacgccg
    14341 cccatgaagc agccgtcgct gcgaacgtcg ccatgttaat gcgcctgcat ggccatgaag
    14401 atctgcaagc caatgcgcaa accgttcttg aggtactgcg cagtggttcc gcttacgaca
    14461 gagttaccgc gctggcggca cgagggtaaa tgatgcaaac cgttttagcg aaaatcgtcg
    14521 cagacaaggc gatttgggta gaaacccgca aacagcagca accgctggcc agttttcaga
    14581 atgaggttca gccgagcacg cgacattttt atgatgcgct acagggtgca cgcacggcgt
    14641 ttattctgga gtgcaagaaa gcgtcgccgt caaaaggcgt gatccgtgat gatttcgatc
    14701 cggcacgcat tgcctccatt tataaacatt acgcttcggc aatttcggtg ctgactgatg
    14761 agaaatattt tcaggggagc tttgatttcc tccccatcgt cagccaaatc gccccgcagc
    14821 cgattttatg taaagacttc attatcgacc cttaccagat ctatctggcg cgctattacc
    14881 aggccgatgc ctgcttatta atgctttcag tactggatga cgaccaatat cgccagcttg
    14941 cagccgtcgc tcacagtctg gagatggggg tgctgaccga agtcagtaat gaagaggaac
    15001 tggagcgcgc cattgcattg ggggcaaagg tcgttggcat caacaaccgc gatctgcgtg
    15061 atttgtcgat tgatctcaac cgtacccgcg agcttgcgcc gaaactgggg cacaacgtga
    15121 cggtaatcag cgaatccggc atcaatactt acgctcaggt gcgcgagtta agccacttcg
    15181 ctaacggttt tctgattggt tcggcgttga tggcccatga cgatttgcac gccgccgtgc
    15241 gccgggtgtt gctgggtgag aataaagtat gtggtctgac gcgtgggcaa gatgctaaag
    15301 cagcttatga cgcgggcgcg atttacggtg ggttgatttt ggttgcgaca tcaccgcgtt
    15361 gcgtcaacgt tgaacaggcg caggaagtga tggctgcggc accgttgcag tatgttggcg
    15421 tgttccgcaa tcacgatatt gccgatgtgg tggacaaagc taaggtgtta tcgctggcgg
    15481 cagtgcaact gcatggtaat gaagatcagc tgtatatcga tacgctgcgt gaagctctgc
    15541 cagcacacgt tgccatctgg aaagcattaa gcgtcggtga aaccctgccc gcccgcgagc
    15601 ttcagcacgt tgataaatat gttttagaca acggccaggg tggaagcggg caacgtttcg
    15661 actggtcact attaaatggt caatcgcttg gcaacgttct gctggcgggg ggcttaggcg
    15721 cagataactg cgtggaagcg gcacaaaccg gctgcgccgg acttgatttt aattctgctg
    15781 tagagtcgca accgggcatc aaagacgcac gtcttttggc ctcggttttc cagacgctgc
    15841 gcgcatatta aggaaaggaa caatgacaac attacttaac ccctattttg gtgagtttgg
    15901 cggcatgtac gtgccacaaa tcctgatgcc tgctctgcgc cagctggaag aagcttttgt
    15961 cagcgcgcaa aaagatcctg aatttcaggc ccagttcaac gacctactga aaaactatgc
    16021 cgggcgtcca accgcactga ccaaatgcca gaacattacg gctggcacga acaccacgct
    16081 gtatctgaag cgcgaagatt tgctgcacgg cggcgcgcat aaaactaacc aggtgctcgg
    16141 tcaggcttta ctggcgaagc ggatgggtaa aaccgaaatc atcgccgaaa ccggtgccgg
    16201 tcagcatggc gtggcgtcgg cccttgccag cgccctgctc ggcctgaaat gccgtattta
    16261 tatgggtgcc aaagacgttg aacgccagtc gcctaacgtt tttcgtatgc gcttaatggg
    16321 tgcggaagtg atcccggtgc atagcggttc cgcgacgctg aaagatgcct gtaacgaggc
    16381 gctgcgcgac tggtccggta gttacgaaac cgcgcactat atgctgggca ccgcagctgg
    16441 cccgcatcct tatccgacca ttgtgcgtga gtttcagcgg atgattggcg aagaaaccaa
    16501 agcgcagatt ctggaaagag aaggtcgcct gccggatgcc gttatcgcct gtgttggcgg
    16561 cggttcgaat gccatcggca tgtttgctga tttcatcaat gaaaccaacg tcggcctgat
    16621 tggtgtggag ccaggtggtc acggtatcga aactggcgag cacggcgcac cgctaaaaca
    16681 tggtcgcgtg ggtatctatt tcggtatgaa agcgccgatg atgcaaaccg aagacgggca
    16741 gattgaagaa tcttactcca tctccgccgg attggatttc ccgtctgtcg gcccacaaca
    16801 cgcgtatctt aacagcactg gacgcgctga ttacgtgtct attaccgatg atgaagccct
    16861 tgaagccttc aagacgctgt gcctacacga agggatcatc ccggcgctgg aatcctccca
    16921 cgccctggcc catgcgctga aaatgatgcg cgaaaacccg gaaaaagagc agctactggt
    16981 ggttaacctt tccggtcgcg gcgataaaga catcttcacc gttcacgata ttttgaaagc
    17041 acgaggggaa atctgatgga acgctacgaa tctctgtttg cccagttgaa ggagcgcaaa
    17101 gaaggcgcat tcgttccttt cgtcacgctc ggggatccgg gcattgagca gtcattgaaa
    17161 attatcgata cgctaattga agccggtgct gacgcgctgg agttaggcat ccccttctcc
    17221 gacccactgg cagatggccc gacgattcaa aacgccactc tgcgcgcctt tgcggcaggt
    17281 gtgactccgg cacaatgttt tgaaatgctg gcactgattc gccagaaaca cccgaccatt
    17341 cccattggcc tgttgatgta tgccaatctg gtgtttaaca aaggcattga tgagttttat
    17401 gcccagtgcg agaaagtcgg cgtcgattcg gtgctggttg ccgatgtgcc aattgaagag
    17461 tccgcgccct tccgccaggc cgcgttgcgc cataatgtcg cacctatctt catctgcccg
    17521 ccaaatgccg atgacgacct gctgcgccag atagcctctt acggtcgtgg ttacacctat
    17581 ttgctgtcac gagcgggcgt gaccggcgca gaaaaccgcg ccgcgttacc cctcaatcat
    17641 ctggttgcga agctgaaaga gtacaacgct gcacctccat tgcaggggtt tggtatttcc
    17701 accccggatc aggtaaaagc agcgattgat gcaggagctg cgggcgcgat ttctggttca
    17761 gccattgtta aaatcatcga gcaacatatt aatgagccag agaaaatgct ggcggcactg
    17821 aaagtttttg tacaaccgat gaaagtggcg acgcgcagtt aatccccctg ccgccagttc
    17881 cgctggcggc attttaactt tctttaacga agccggaaaa atcctaaatt catttaatat
    17941 ttatcttttt accgtttcgc t
//
LOCUS       DAJTZL010000082        17448 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.082, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000082 DAJTZL010000000
VERSION     DAJTZL010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17448
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.082"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..502)
                     /locus_tag="OO544_002363"
     CDS             complement(<1..502)
                     /locus_tag="OO544_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001642584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HCR6767944.1"
                     /translation="MALTDIKVRTAKPTDKQYKLTDGGGMHLLVHPNGSKYWRLQYRY
                     EGKQKMLALGVYPEITLADARIRRDEPRKLLANGVDPGDKKKSDKVEQSKARTFKEVA
                     IEWHGTNKKWSEDHAHRVLKSLEDNLFAALGERNIAELKTRDLLAPIKAVEMSGRLEM
                     AARLQQR"
     gene            complement(688..772)
                     /locus_tag="OO544_002364"
     tRNA            complement(688..772)
                     /locus_tag="OO544_002364"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(736..738),aa:Leu,seq:caa)
     gene            968..1987
                     /gene="ahr"
                     /locus_tag="OO544_002365"
     CDS             968..1987
                     /gene="ahr"
                     /locus_tag="OO544_002365"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HCR6767945.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYEPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKTLAGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSVSGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRNGKARYRVV
                     LKADF"
     gene            complement(1991..2554)
                     /gene="idnK"
                     /locus_tag="OO544_002366"
     CDS             complement(1991..2554)
                     /gene="idnK"
                     /locus_tag="OO544_002366"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HCR6767946.1"
                     /translation="MAGESFILMGVSGSGKTLIGSKVAALLSAKFIDGDDLHPAKNID
                     KMSQGIPLSDEDRLPWLERLNDASYSLYKKNETGFIVCSSLKKQYRDILRKGSPHVHF
                     LWLDGDYETILARMQRRAGHFMPVALLKSQFEALERPQADEQDIVRIDINHDIANVTE
                     QCRQAVLAIRQDRICAKGGSASDQRCE"
     gene            2771..3802
                     /gene="idnD"
                     /locus_tag="OO544_002367"
     CDS             2771..3802
                     /gene="idnD"
                     /locus_tag="OO544_002367"
                     /EC_number="1.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-idonate 5-dehydrogenase"
                     /protein_id="HCR6767947.1"
                     /translation="MQVKTQSCVVADKKTVAVTEQTIDWNNNGTLVQITRGGICGSDL
                     HYYQEGKVGNFMIKAPMVLGHEVIGKVIHSDSSELHEGQTVAINPSKPCGHCKYCIEH
                     NENQCTDMRFFGSAMYFPHVDGGFTRYKMVETSQCVPYPAKADEKVMAFAEPLAVAIH
                     AAHQADELQGKRVFVSGVGPIGCLIVSAVKTLGSAEIVCADVSPRSLSLAKEMGADVL
                     VNPQNDDMDHWKAEKGHFDVSFEVSGHPSSVNTCLEVTRARGVMVQVGMGGAMAEFPM
                     MTLIGKEISLKGSFRFTSEFNTAVSWLANGVINPLPLLSAEYLFTDLEEALRFAGDKT
                     QAAKVQLVF"
     gene            3826..4590
                     /gene="idnO"
                     /locus_tag="OO544_002368"
     CDS             3826..4590
                     /gene="idnO"
                     /locus_tag="OO544_002368"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HCR6767948.1"
                     /translation="MNDLFSLAGKNILITGSAQGIGYLLATGLGKYGAQIIINDITAE
                     RAELAVEKLHQEGIQAVAAPFNVTHKHEIDAAVEHIEKDIGPIDVLVNNAGIQRRHPF
                     TEFPEQEWNDVIAVNQTAVFLVSQAVTRHMVERKAGKVINICSMQSELGRDTITPYAA
                     SKGAVKMLTRGMCVELARHNIQVNGIAPGYFKTEMTKALVEDEAFTAWLCKRTPAARW
                     GDPQELIGAAVFLSSKASDFVNGHLLFVDGGMLVAV"
     gene            4655..5974
                     /gene="idnT"
                     /locus_tag="OO544_002369"
     CDS             4655..5974
                     /gene="idnT"
                     /locus_tag="OO544_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gnt-II system L-idonate transporter"
                     /protein_id="HCR6767949.1"
                     /translation="MPLIIIAAGVALLLILMIGFKVNGFIALVLVAAVVGFAEGMDAQ
                     AVLHSIQNGIGSTLGGLAMILGFGAMLGKLISDTGAAQRIATTLIATFGKKRVQWALV
                     ITGLVVGLAMFFEVGFVLLLPLVFTIVASSGLPLLYVGVPMVAALSVTHCFLPPHPGP
                     TAIATIFEANLGTTLLYGFIITIPTVIVAGPLFSKLLTRFEKAPPEGLFNPHLFSEEE
                     MPSFWNSIFAAVIPVILMAIAAVCEITLPKTNTVRLFFEFVGNPAVALFIAIVIAIFT
                     LGRRNGRTIEQIMDIIGDSIGAIAMIVFIIAGGGAFKQVLVDSGVGQYISHLMTGTTL
                     SPLLMCWTVAALLRIALGSATVAAITTAGVVLPIINVTHADPALMVLATGAGSVIASH
                     VNDPGFWLFKGYFNLTVGETLRTWTVMETLISIMGLLGVLAINAVLH"
     gene            6041..7039
                     /gene="idnR"
                     /locus_tag="OO544_002370"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
     CDS             6041..7039
                     /gene="idnR"
                     /locus_tag="OO544_002370"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator IdnR"
                     /protein_id="HCR6767950.1"
                     /translation="MRNHRISLQDIATLAGVTKMTVSRYIRSPKKVAKETGERIAKIM
                     EEINYIPNRAPGMLLNAQSYTLGILIPSFQNQLFADILAGIESVTSVHNYQTLIANYN
                     YDRDSEEESVINLLSYNIDGIILSEKYHTIRTVKFLRSATIPVVELMDVQGERLDMEV
                     GFDNRQAAFDMVCTMLDKRVRRKILYLGSKDDTRDEQRYQGYCDAMMLHNLSPLRMNP
                     RAISSIHLGMQLMRDALSANPDLDGVFCTNDDIAMGALLLCRERNLAVPEQISIAGFH
                     GLEIGRQMIPSLASVITPRFDIGRMAAQMLLSKIKNNDHNHNTVDLGYQIYHGNTL"
     gene            7117..8619
                     /locus_tag="OO544_002371"
     CDS             7117..8619
                     /locus_tag="OO544_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HCR6767951.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLARLKNIGVNDWQPHANPVVVWDIFG
                     EKGHPVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAEHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRKR"
     gene            complement(8738..9820)
                     /gene="lptG"
                     /locus_tag="OO544_002372"
     CDS             complement(8738..9820)
                     /gene="lptG"
                     /locus_tag="OO544_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HCR6767952.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVASQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(9820..10920)
                     /gene="lptF"
                     /locus_tag="OO544_002373"
     CDS             complement(9820..10920)
                     /gene="lptF"
                     /locus_tag="OO544_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HCR6767953.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVVLDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            11187..12698
                     /gene="pepA"
                     /locus_tag="OO544_002374"
     CDS             11187..12698
                     /gene="pepA"
                     /locus_tag="OO544_002374"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HCR6767954.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            12832..13275
                     /gene="holC"
                     /locus_tag="OO544_002375"
     CDS             12832..13275
                     /gene="holC"
                     /locus_tag="OO544_002375"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HCR6767955.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            13275..16130
                     /gene="valS"
                     /locus_tag="OO544_002376"
     CDS             13275..16130
                     /gene="valS"
                     /locus_tag="OO544_002376"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HCR6767956.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGADVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(16186..17382)
                     /locus_tag="OO544_002377"
     CDS             complement(16186..17382)
                     /locus_tag="OO544_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 domain-containing protein"
                     /protein_id="HCR6767957.1"
                     /translation="MAQVINDMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFFYFAILMTVSADMPLIGCV
                     LTLSLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSMKGFWWVTFFLPILMALGMGTV
                     FFISTKMLHANSSSSVIISVVLMTIVCIVFIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTTYCIKYAILAFLALLPFLAVAGYIIFDQILNEYDSSGYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGIM
                     PSLPFL"
BASE COUNT         4484 a         4335 c         4453 g         4176 t
ORIGIN      
        1 tgcgctgttg aagacgagca gccatttcaa gacgtccaga catttctacg gccttaatag
       61 gtgctaataa atctcgagtt tttaactcag cgatattacg ttcaccaagc gctgcaaaaa
      121 gattatcttc cagacttttt agcacacgat gggcatgatc ttcagaccac tttttattgg
      181 tgccatgcca ctcaatcgcg acttctttaa aggttcgtgc tttactctgt tcaaccttat
      241 cacttttctt tttgtctccc ggatcgacgc cattcgcaag cagcttacgt ggctcgtcac
      301 gacgtattct ggcatccgcg agtgtgattt caggataaac cccaagtgcc agcatctttt
      361 gctttccctc ataacggtac tgcaaacgcc agtacttaga accatttgga tggacaagca
      421 gatgcatacc gccaccatca gtcaacttat attgcttatc tgttggctta gctgttctga
      481 ctttgatgtc tgttaatgcc atgcgtattc cctccctgtt ggtacaagca ttatcgaacc
      541 ggaaatacca tcatctgtac caacatatgt tggtagaagt acgttgatat cggttgacct
      601 tgagagagtt aatatagcgg gaaaggtaga taaatactga atttcaggca taaaaaagac
      661 ctcagttgag gtctattgac atacttttgg tgccgaaggc cggactcgaa ccggcacgta
      721 tttctacggt tgattttgaa tcaactgcgt ctaccgattt cgccacttcg gcactgaaga
      781 ggtatgcgga aaacgttgtg gattatacct gtcgcacgcc accatgcaag cgccagcttg
      841 ctcgcacctc gctaagtgct gaaattttca gcattgcata cagcgatgtg taacctttgt
      901 cacactccag gcaccccgcc ctgccatgct ctacacttcc cagacaacac cagagaagga
      961 ccaaaaaatg tcgatgataa aaagctatgc cgcaaaagaa gcgggcggcg aactggaagt
     1021 ttatgagtac gagcccggtg aattgaagcc acaagatgtt gaagtgcagg tggattactg
     1081 cgggatctgc cattccgatc tgtcgatgat cgacaacgaa tggggatttt cacagtatcc
     1141 gctggttgcc gggcatgagg tgattgggcg cgtggtggcg ctcgggagcg ccgcgcagga
     1201 taaaggtttg caggtcggtc agcgtgtcgg gattggctgg acggcgcgta gctgcgggca
     1261 ttgcgacgcc tgtattagcg gtaatcagat caactgcgag caaggtgcgg tgccaacaat
     1321 tatgaatcgc ggcggttttg ccgagaagtt gcgtgcagac tggcaatggg tgatcccact
     1381 gccggaaaat atcgatatcg agtccgccgg gccgctgctg tgtggtggta tcacggtctt
     1441 taaaccactg ttgatgcacc atatcactgc taccagccgc gttggggtaa ttggtattgg
     1501 cgggctgggg catatcgcta taaaacttct gcacgcaatg ggatgcgagg tgacagcctt
     1561 tagttcgaat cctgcgaaag agcaggaagt actggcgatg ggtgccgata aagtggtgaa
     1621 tagccgcgat ccgcaggcac tgaaaacact tgcggggcag ttcgatctca ttatcaacac
     1681 cgtcaacgtc agcctcgact ggcagcctta ttttgaggcg ctgacgtatg gcggtaattt
     1741 ccacaccgtc ggtgcggtcc tcacgccgct gtctgttccg gcctttacgt taattgcggg
     1801 cgaccgcagc gtctctggtt cagcaaccgg cacaccttat gagcttcgta agctgatgcg
     1861 ctttgcggcc cgcagcaagg ttgcgccgac caccgaactg ttcccgatgt cgaaaataaa
     1921 cgacgccatc cagcatgtgc gcaacggtaa ggcgcgttac cgcgtggttc tgaaagccga
     1981 tttttgaaaa tcattcgcag cgctgatctg aggcgctacc ccctttcgca catattctgt
     2041 cttgtcgtat cgccagcacg gcctgccgac attgttcggt gacattggca atatcatggt
     2101 tgatatcaat gcgtacaata tcctgttcat ctgcttgtgg acgctccagc gcctcaaact
     2161 gactttttag taacgctacc ggcataaaat gcccagcccg ccgctgcatt cgcgcgagaa
     2221 tagtttcata gtcgccatct aaccagagga aatgaacatg ggggctaccc ttgcgtaaaa
     2281 tatcacgata ctgttttttt aatgatgaac agacaataaa tcctgtttca ttctttttat
     2341 aaagactgta tgaagcatca tttaagcgtt ccagccaggg aagtcgatct tcatcagata
     2401 atggaatacc ctgcgacatt ttatctatat ttttggctgg atgaagatcg tcaccatcaa
     2461 taaatttagc agataataac gcggcaacct tgctaccaat taatgtttta ccactccctg
     2521 aaacgcccat caaaataaag ctttcacccg ccatttaaaa tctcctgaat catcttacct
     2581 gctgcgatac tacgcctggt ggcataataa tttcacgttt ttttatttct gctgataaga
     2641 attacaaggc acatcacgtt atgcgtaaca tagtaatgta acaattttct gacgtgatct
     2701 tcatcacaaa taatgacaat taaaaccgct taaatgcttc cgaggtgtct acctgaccag
     2761 tgaggaattt atgcaagtga aaacacagtc ctgcgttgtt gcggacaaga aaactgttgc
     2821 cgttaccgag cagacgatag attggaataa taatggaaca ttagtacaaa taacccgagg
     2881 tggaatttgc ggttccgatt tacattatta tcaggaagga aaagtaggta atttcatgat
     2941 aaaggcaccg atggtgttag gtcatgaagt tatcggtaaa gttattcata gcgactcatc
     3001 agaattacat gaagggcaaa cggtagccat taatccgtct aaaccgtgcg gtcactgcaa
     3061 atactgcatt gaacataacg agaatcagtg tacagatatg cgtttttttg gcagtgccat
     3121 gtatttccct catgttgatg gtggttttac ccgttataaa atggtcgaaa cgtcgcaatg
     3181 tgtcccttat ccggccaaag ctgacgaaaa ggttatggct tttgccgaac ctttagccgt
     3241 cgcgattcat gccgcacatc aggccgacga gttacagggc aagcgagtat ttgtttccgg
     3301 tgttgggccc attggctgcc tgattgtcag tgctgtgaaa acactggggt ccgcggaaat
     3361 tgtctgtgcc gatgtgagtc cccgttccct ttcgctggcc aaagagatgg gtgcggatgt
     3421 gctcgtaaac ccacaaaacg acgacatgga tcactggaaa gcggaaaaag gccatttcga
     3481 tgtcagcttt gaagtgtccg gtcatccttc atcagtgaat acctgtctgg aggtcactcg
     3541 tgcacgcggc gtaatggtgc aggtagggat gggaggcgcg atggcagaat tcccaatgat
     3601 gacgttgatt ggtaaggaga tttcactcaa aggctctttc cgttttacca gcgaatttaa
     3661 taccgcagtg tcatggctgg cgaatggcgt tatcaatcca ctgcctttac tgagtgctga
     3721 atatctcttc actgacctgg aagaggcgct acgtttcgcc ggtgataaaa cccaggcagc
     3781 aaaagtccag cttgttttct aagagataaa taaaggaata atacaatgaa cgatctattt
     3841 tcactggcag gaaaaaatat cttgattacc ggttcagcac agggcattgg ctatttactg
     3901 gcaaccggcc tgggtaaata tggcgcacaa ataattatta atgatattac tgccgaacgc
     3961 gcagaacttg ctgtagaaaa actccaccag gagggtattc aggccgttgc cgcacctttt
     4021 aatgttactc ataaacatga aattgatgcc gccgttgaac atatcgaaaa ggacatcggc
     4081 cccattgatg tgctggtgaa taacgccggt atccagcgcc gtcatccttt tactgagttc
     4141 cctgaacaag agtggaatga tgtgatcgca gtaaaccaga ccgccgtgtt cctggtatcg
     4201 caagcggtaa ctcgtcacat ggttgaacgc aaggcaggta aagttattaa tatttgctcg
     4261 atgcaaagcg aactgggacg tgacaccatc accccttatg ccgcatcgaa aggggcggta
     4321 aaaatgctca cccgcggcat gtgcgtcgag ctggcgcgcc acaatattca ggtcaacggt
     4381 attgcgccgg gctatttcaa aacagaaatg actaaagcac tggttgagga cgaagccttc
     4441 accgcctggt tgtgcaaacg gacccccgcc gcacgctggg gagatccgca ggaactgatt
     4501 ggtgctgcgg tgttcctttc ttcaaaagcc tctgatttcg taaacggcca cctgttgttt
     4561 gttgatggcg gcatgttagt ggctgtttaa cagccaccac cctacgtgca gcgcgaaagc
     4621 gctgcttttc tggcactaat aagagataaa gactatgcca ttaatcatta ttgcggcagg
     4681 cgtcgcgctg cttcttatcc tgatgatcgg ctttaaagtt aacggcttta ttgccctcgt
     4741 tctggtagct gccgtcgtcg gatttgccga agggatggat gcacaggccg tcctgcactc
     4801 tatacaaaat ggtatcggca gcacgctcgg agggctggca atgatcctcg gtttcggggc
     4861 catgttaggc aagctgattt ctgatacggg cgcggcacaa cgtatcgcca ctacgctgat
     4921 tgctactttt ggtaaaaaac gcgtgcaatg ggcgctagtg atcaccggtc tggttgtggg
     4981 cctcgccatg ttttttgaag tgggttttgt cctgctgttg ccgttggtat ttaccatcgt
     5041 ggcatcatct ggattacccc tgttgtatgt tggcgtaccg atggtagcag cgctctctgt
     5101 aacccactgt tttctgccgc cacatccagg gcctactgcc atcgcgacta tctttgaggc
     5161 taatctcgga acgactttac tgtatggatt tatcattacc attccgacag ttattgtcgc
     5221 aggaccgctg ttttctaaac tgctaactcg ctttgagaaa gcaccaccgg aaggcttatt
     5281 taatcctcat ctgtttagcg aagaggagat gccctccttc tggaacagta tttttgctgc
     5341 agtgatcccg gtcatcctga tggctatcgc cgccgtttgt gaaattacgt taccgaaaac
     5401 taacaccgtg cgcctcttct ttgaatttgt cggtaaccct gccgttgcgc tgtttattgc
     5461 cattgttatt gcgattttca cactgggccg acgtaatgga cgcaccatcg agcaaatcat
     5521 ggatatcatt ggggattcta taggcgctat cgcgatgatt gtgtttatta tcgctggcgg
     5581 cggcgcgttt aagcaggtat tagtagatag tggtgtcggg cagtatattt cacacttaat
     5641 gaccggaact acgctttcgc cgttattgat gtgctggact gtcgcggcgc tgttgcgtat
     5701 cgctctgggc tctgccaccg tcgcggccat taccaccgcg ggtgtggtgt tgccgattat
     5761 caacgttacc catgccgatc ccgctttaat ggtactggca actggtgcgg gcagcgtgat
     5821 cgcgtcacac gtaaacgacc ctggcttctg gctatttaaa gggtatttta atctgacggt
     5881 tggtgaaacg ttgcgtacct ggacagtgat ggaaaccctt atttctatta tgggtttgct
     5941 gggcgtgtta gccattaacg ccgtattgca ctgaaagaat aaaatattgt gatgtagttt
     6001 cgggggaaga ttgaagattc ccccgacgtt gggagaagtt atgcgcaatc acagaatttc
     6061 tttacaggat atcgctacgc tggctggcgt aacaaaaatg accgtgagtc gttatatccg
     6121 ctcgccgaaa aaggtggcaa aggaaacagg cgagcgcatc gccaaaatta tggaagagat
     6181 caattatatc cctaatcgcg ctcccggcat gttgttgaat gcacaaagtt ataccctcgg
     6241 tatcttgatt ccgtcatttc aaaatcaact cttcgctgac attctcgcgg gaatagaatc
     6301 cgtcacctct gtgcataatt atcagaccct tattgctaat tacaattacg atcgcgattc
     6361 cgaagaagag tcggtgatta acctgctctc ttataacatt gacgggatta ttctttcgga
     6421 aaaatatcac accatcagga cggtgaaatt tctgcgttca gccaccattc cggtcgtgga
     6481 attgatggat gtacagggag aacggctgga tatggaggtc ggttttgata atcggcaggc
     6541 tgcttttgac atggtgtgta ccatgctgga taagcgagtc aggcgcaaaa ttctgtatct
     6601 cggttccaaa gatgacaccc gcgatgaaca gcgttatcag gggtattgcg atgcgatgat
     6661 gctgcataat ctttccccat tacgcatgaa tccacgcgcc atctcatcca ttcatttagg
     6721 aatgcagtta atgcgtgatg cccttagcgc aaacccggat ttagacggtg tattttgcac
     6781 caatgacgat attgcgatgg gtgcgcttct gctgtgccgc gagcgaaacc tcgccgtgcc
     6841 agaacaaatt tccattgcgg gttttcatgg gctggagatt ggcaggcaaa tgatcccaag
     6901 cctcgccagc gtaattactc cccgttttga tattggtcgg atggccgccc aaatgttgct
     6961 gagcaagatt aagaataacg atcacaacca caacactgtt gacctcgggt atcagattta
     7021 tcacggtaac accctttagg ttattttctc ttcattacac atattctctt tgccggacgt
     7081 tgcctatact ccaggcagga aactggagga aatctcatga gcgaacccct gttaattgcc
     7141 cgcacgccgg acacagaact gtttttactg ccgggaatgg ctaaccgtca cgggctgatt
     7201 actggcgcaa cggggacggg taaaactgtc acgttgcaaa aactggcaga gtcattgtcg
     7261 gaaatcggtg ttccggtgtt tatggccgat gtgaaaggcg atctgaccgg catcgcgcag
     7321 gcaggaacgg cgtcggaaaa actgctcgca agactgaaaa atatcggcgt caacgactgg
     7381 caaccgcatg ccaatccggt ggtggtgtgg gatatctttg gcgagaaagg ccatccggtg
     7441 cgggcgacgg tttcggatct ggggccgctg ttgctggcac gactgttgaa tctcaacgat
     7501 gtgcaatctg gcgtgctgaa tattatcttc cgcattgcgg acgatcaggg attgttgctg
     7561 ctcgacttta aagatttgcg ggcgattacc cagtacatcg gcgataacgc caaatccttc
     7621 cagaatcagt acggaaatat cagtagcgca tcggttggtg ccatccagcg cggattactg
     7681 tcgctggaac agcaaggcgc agaacacttc tttggtgagc cgatgctgga tatcaaagac
     7741 tggatgcgca ccgatgccaa cggtaaaggc gttatcaata tcctcagcgc cgagaaactt
     7801 tatcagatgc cgaaactgta cgccgccagc ctgctgtgga tgctttcaga gttatatgaa
     7861 caattgcctg aagcgggcga tctggagaaa ccaaaactgg tgtttttctt cgacgaagca
     7921 catctgctgt ttaacgatgc gccacaggta ctgctggata agattgagca ggtgataagg
     7981 cttattcgct caaaaggcgt gggcgtctgg ttcgtttcgc aaaacccgtc agatatcccg
     8041 gataacgtgc tagggcagct cggtaatcgc gttcaacacg ctttgcgtgc ttttacgcct
     8101 aaagatcaga aagcagtgaa ggcagcggcg caaaccatgc gggccaatcc ggcgtttgat
     8161 accgaaaagg caatccagga actggggacc ggcgaggcgt taatctcgtt tctcgatgcg
     8221 aaaggaagtc cttcagtggt ggaacgggcg atggtgatcg caccttgttc gcgaatgggg
     8281 ccggtgacgg aagatgagcg taatggcctg attaatcact ccccggtgta tggcaaatat
     8341 gaagatgagg tggaccgcga gtccgcctat gagatgctgc aaaaaggctt tcaggccagt
     8401 accgagcagc aaaataatcc ccccgtgaaa gggaaagagg tggcggtgga tgacggtatt
     8461 cttggtggat tgaaggatat tttgtttggc actaccggac cacgcggcgg gaagaaagat
     8521 ggtgtggtgc aaacaatggc gaaaagcgcc gctcgccagg tgacgaatca gattgtacgc
     8581 gggatgttgg ggagtttgct gggggggaga aaaaggtaag tcagacgtga tctgtgcccc
     8641 tcaccctaac cctctcccca aaggggcgag gggaccgttc gaactcgtat ttgactttgt
     8701 caacaataat aaagggagct ttcgctccct tgttagatta cgattttctc attaacagcc
     8761 acaggctgat taagaagaag ctggcgcttg gcaacagtgc gccgatgatc ggcgggatgc
     8821 cataaaccaa cgtcagcggg ccgaagatct ggtccagtac gtagaagaca aaaccgaagc
     8881 tgataccggt gaccacacgc acgcccatcg gtacgctacg cagtgggcca aagatgaacg
     8941 acagcgccat caacatcatc accgccacgg atagcggctg gaagattttg ctccacatgt
     9001 tgagctgata acgtccggca tcctgaccgc tcgacttcag atacttcaca tagttgtgca
     9061 aaccactgat ggagagtgca tccgggtcca gcgccaccac gcccagtttg tctggcgtga
     9121 ggttggtttt ccaggtgccg ctcaccgtct gcgaaccggt gatctgtttc ggattggtca
     9181 gatcagattc atcaacctgc gacagacgcc agactttatg ttccgggtca aacttcgcgg
     9241 tagcggcata acgtacggat tgcagacgac gattctcgtt aaaggcataa atactgatgc
     9301 cacctaactc ttcgtcacct ttaacccgct caatgtagac gaagttgttg ccatctttcg
     9361 cccataagcc ttgctgagtg gagagcaacg agccgccgta catcgcctgc gcacggtagt
     9421 tacgcgccat ctgctcgccc tgcgacgcga cccattcacc aatcgccatc gtcagcaaga
     9481 ccagcggaat ggcggttttc atcaccgaca gcgccacctg catacgggta aaaccagaag
     9541 cctgcatcac caccagttcg ctgcgctgcg ccagcatccc aagaccaagc aacgccccaa
     9601 gcagagccgc catcgggaag aagatttgca catctttcgg cacgctcagc aaggtataca
     9661 ttcctgcgcc taacgcgtcg taactcccct gcccggcttt tttcagctga tcgacaaact
     9721 tgataatgcc cgacagcgac accagcatga acagcgtcat catgatggtg gtgaaaatag
     9781 ttttaccgat atagcggtca agtacgccaa aaggttgcat cacaccgctc ctttacgcga
     9841 aaaactggcg cgcaggcggc ggaccggcac ggtgtcccaa aggttgagaa caatcgccaa
     9901 agccagataa atcaggttaa cggtccacat ccacagcgtc gggtccagct tacctttacc
     9961 gccgttcgat ttcagggagg tctggatcag gaagaaaagt agatacagca gcatggctgg
    10021 cagcatcgac agtacgcgtc cctgacgcgg gttaaccacg ctcaacggta caaccataag
    10081 tgccatcata aacacggtga ataccaacgt gatacgccag ttcagttctg cgcgggcacg
    10141 atcggtgtca gtgttccaca atgtgcgcat gtccatctgg tcggtatcgt tcgggtcgag
    10201 caccaccgcc tggtgaccaa tgatcgcctg ataatcctga aagtccgtaa tgcggaaatc
    10261 gcgtaacagt gcagtgcctt cgaagcgcgt tccctggttg agagtgacga cctgggagcc
    10321 gtcgcgcagc tgggttaaat gtccggaatc ggccaccacc acagaaggac gtgcattacc
    10381 ttttggtcga atttgtgcga ggaacacatc tttgaaatcg ctgccgtcaa cgctttcgat
    10441 gaacagcacc gagctgccat tagtcgcttg ctggaattgc ccttgcgcca gcgccgccat
    10501 gccagggttc gctttcgctt ctgctaacac ttcatcctga tgacgcgatg accatggtcc
    10561 cgcccacatc acgttaaccg ccgcgacgat tgccgtgaac accgcaagga tcattgccgc
    10621 tttcaccagc accgctttgc tcagaccgca ggcatgcatt accgtaattt cactttcggt
    10681 atacagtttg cccagcgtca tcagcagccc gaggaacagg cttaatggca ggataagctg
    10741 cgccatttcc ggcacgccca acccgagaag ggagagcacc agattcgccg gaatatcgcc
    10801 gtcaaccgct gcgccgagga tcctcactaa cttttgacag aagaagatca aaagcaagat
    10861 gaagagtatc gccagctggc ttttgagcgt ctcccgcacc agatatctta tgattatcac
    10921 tttaaatacg cccgtaaaaa ctcgtctttt gcaggatttt agcttgtttc atggcttaaa
    10981 cgtcatttat tctcttgagt cgtcgaaatc gtcgctaaga taattatact caacggattc
    11041 acctctcaga ttttgttctg acgtgccaat gccgtaataa cgttaagatt aacacgaagt
    11101 catcgcaaca gcggacatga gttacgaaag cttgcaattc tatctgtagc caccgccgtt
    11161 gtctttaaga ttcaggagcg tagtgcatgg agtttagtgt aaaaagcggt agcccggaga
    11221 aacagcggag tgcctgcatc gtcgtgggcg tcttcgaacc acgtcgcctt tctccgattg
    11281 cagaacagct cgataaaatc agcgatgggt acatcagcgc cctgctacgt cggggcgaac
    11341 tggaaggaaa accggggcag acactgttgc tgcaccatgt tccgaatgta ctttccgagc
    11401 gaattctcct tattggttgc ggcaaagaac gtgagctgga tgaacgtcag tacaagcagg
    11461 ttattcagaa aaccattaat acgctgaatg atactggctc aatggaagcg gtctgctttc
    11521 tgactgaact gcacgttaaa ggccgtaaca actactggaa agtgcgtcag gctgtcgaga
    11581 ctgcaaaaga gacgctctac agtttcgatc agctgaaaac gaacaagagc gaaccgcgtc
    11641 gtccgctgcg taaaatggtg ttcaacgtgc cgacccgccg tgaactgacc agcggtgagc
    11701 gcgcgatcca gcacggtctg gcgattgccg ccgggattaa agcagcaaaa gatctcggca
    11761 atatgccgcc gaatatctgt aacgccgctt acctcgcttc acaagcgcgc cagctggctg
    11821 acagctacag caagaatgtt atcacccgcg ttatcggcga acagcagatg aaagagctgg
    11881 ggatgcactc ttatctggcg gtcggtcagg gttcgcaaaa cgaatcgctg atgtcggtga
    11941 ttgagtacaa aggcaacgcg tcggaagatg cacgcccaat cgtgctggtg ggtaaaggtt
    12001 taaccttcga ctccggcggt atctccatca agccttcaga aggcatggat gagatgaagt
    12061 acgatatgtg cggtgcggca gcggtttacg gcgtgatgcg gatggtcgca gagctacaac
    12121 tgcctattaa cgttattggc gtgctggcag gctgtgaaaa catgcctggc gggcgtgcct
    12181 atcgtccggg cgatgtgtta accaccatgt ccggtcaaac cgttgaagtg ctgaacaccg
    12241 atgccgaagg ccgcctggta ctgtgcgacg tgttaactta cgttgaacgt tttgagccgg
    12301 aagcggtgat tgatgtggcg acgctgaccg gtgcctgcgt gatcgcgctg ggtcatcata
    12361 ttactggtct gatggcgaac cataatccgc tggcccatga actgattgcc gcgtctgaac
    12421 aatccggtga ccgcgcatgg cgcttaccgc tgggtgacga gtatcaggaa caactggagt
    12481 ccaattttgc cgatatggcg aacattggcg gtcgtcctgg tggggcgatt accgcaggtt
    12541 gcttcctgtc acgctttacc cgtaagtaca actgggcgca cctggatatt gcaggaaccg
    12601 cctggcgttc tggtaaagca aaaggcgcaa ccggtcgtcc ggtagcgttg ctggcacagt
    12661 tcctgttaaa ccgcgctggg tttaacggcg aagagtaagt ttgcgtttta ccctcaccct
    12721 aaccctctcc ctaagggaga gggaaccgat cgagcgcaat agaaattcat tgctcatctt
    12781 tcagggtgag gaatgcagca atatttaaat ccaccacaag aagccccata tatgaaaaac
    12841 gcaacgttct accttctgga caatgacacc accgtcgatg gcttaagcgc cgttgagcaa
    12901 ctggtgtgtg aaattgccgc agaacgttgg cgcagcggta agcgcgtgct catcgcctgt
    12961 gaagatgaaa agcaggctta ccggctggat gaagccctgt gggcgcgtcc ggcagaaagc
    13021 tttgtcccgc ataatttagc gggagaagga ccgcgcggcg gtgcgccggt ggagatcgcc
    13081 tggccgcaaa aacgcagcag cagcccgcgc gatatattga ttagtctgcg aacaagcttt
    13141 gcagattttg ccactgcttt cacagaagtg gtagacttcg ttccttatga agattctctg
    13201 aaacaactgg cgcgcgaacg ctataaagcc taccgcgtgg ctggtttcaa cctgaatacg
    13261 gcaacctgga aataatggaa aagacatata acccacaaga tatcgaacag ccgctttacg
    13321 agcactggga aaagcagggc tactttaagc ctaatggcga tgaaagccag gaaagtttct
    13381 gcatcatgat cccgccgccg aacgtcaccg gcagtttgca tatgggtcac gccttccagc
    13441 aaaccatcat ggataccatg atccgctatc agcgcatgca gggcaaaaac accctatggc
    13501 aggtcggtac tgaccacgcc gggatcgcta cccagatggt cgttgagcgc aagattgccg
    13561 cagaagaagg taaaacccgt cacgactacg gccgcgaagc tttcatcgac aaaatctggg
    13621 aatggaaagc ggagtccggc ggcaccatta cccgtcagat gcgccgtctc ggcaactctg
    13681 tcgactggga gcgtgaacgc ttcacgatgg acgaaggcct gtccaatgcg gtgaaagaag
    13741 ttttcgttcg tctgtataaa gaagacctga tttaccgtgg caaacgcctg gtaaactggg
    13801 atccgaaact gcgcaccgct atctctgacc tggaagtgga aaaccgcgaa tcgaaaggtt
    13861 cgatgtggca catccgctat ccgctggctg acggcgcgaa aaccgcagac ggtaaagatt
    13921 atctggtggt tgcgactacc cgtccggaaa ccctgttggg cgataccggc gtggccgtta
    13981 acccggaaga tccgcgttac aaagacctga ttggcaaata tgtcattcta ccgctggtta
    14041 accgtcgtat tccgatcgtt ggcgacgaac acgccgacat ggaaaaaggc accggctgcg
    14101 tgaaaatcac tccggcgcac gactttaacg actatgaagt gggtaaacgt cacgcgctgc
    14161 cgatgatcaa catcctgacc tttgacggcg atatccgtga aagcgcccag gtgttcgata
    14221 ccaaaggtaa cgaatctgac gtttattcca gcgaaatccc ggcagagttc cagaaactcg
    14281 agcgttttgc tgcacgtaaa gcagtcgttg ctgcggttga cgcactcggc ctgctggaag
    14341 aaattaaacc gcacgacctg accgttcctt acggcgaccg tggcggcgta gttatcgaac
    14401 caatgctgac cgaccagtgg tacgtgcgtg ccgatgtact ggcgaaaccg gcggttgaag
    14461 cggttgagaa cggcgacatt cagttcgtac cgaagcagta cgaaaacatg tacttctcct
    14521 ggatgcgcga tattcaggac tggtgtatct ctcgtcagct gtggtggggt caccgtatcc
    14581 ctgcatggta cgacgaagca ggtaacgttt atgttggccg caacgaagaa gaagtgcgca
    14641 aggaaaataa cctcggtgcc gacgttgccc tgcgtcagga cgaagacgtt ctcgacacct
    14701 ggttctcttc tgcgctgtgg accttctcta ccctaggctg gccggaaaac actgacgcac
    14761 tgcgccagtt ccacccaacc agcgtgatgg tatccggctt cgacatcatt ttcttctgga
    14821 ttgcccgcat gatcatgatg accatgcact tcatcaaaga tgaaaatggc aaaccgcagg
    14881 tgccgttcca caccgtttac atgaccggcc tgattcgtga cgacgaaggc cagaagatgt
    14941 ccaaatccaa gggtaacgtt attgacccgc tggatatggt tgacggtatt tcgctgccgg
    15001 aactgctgga gaaacgtacc ggcaatatga tgcagccgca gctggcggac aaaatccgta
    15061 agcgcaccga gaagcagttc ccgaacggta ttgagccgca cggcaccgat gccctgcgct
    15121 tcaccctggc ggcgctggcg tctaccggtc gtgacatcaa ctgggatatg aagcgcctgg
    15181 aaggttaccg taacttctgt aacaagctgt ggaacgccag ccgctttgtg ctgatgaaca
    15241 cggaaggtca ggattgcggc ttcaacggtg gtgaaatgac gctgtcgctg gcggaccgct
    15301 ggattctggc agagttcaac cagaccatca aagcgtaccg cgaagcgctg gacagcttcc
    15361 gcttcgatat cgctgcaggc attctgtatg agttcacctg gaaccagttc tgtgactggt
    15421 atctggagct gactaagccg gtaatgaacg gtggcaccga agccgaactg cgcggtactc
    15481 gccatacgct ggtgaccgta ctggaaggtc tgctgcgcct cgcgcatccg atcattccgt
    15541 tcatcaccga aaccatctgg cagcgtgtga aagtactttg cggtatcact gccgacacca
    15601 tcatgctgca gccgttcccg cagtacgatg catctcaggt tgatgaagcc gcactggccg
    15661 acaccgaatg gctgaaacag gcgatcgttg cggtacgtaa catccgtgca gaaatgaata
    15721 tcgcgccggg caaaccgctg gagctgctgc tgcgtggttg cagcgcggat gcagaacgtc
    15781 gcgtaaatga aaaccgtggc ttcctgcaaa ccctggcgcg tctggaaagc atcaccgtgc
    15841 tgcctgccga tgacaaaggt ccggtttccg ttacgaagat catcgacggc gcagagctgc
    15901 tgatcccgat ggctggcctc atcaacaaag aagatgagct ggcgcgtctg gcgaaagaag
    15961 tggcgaagat cgaaggcgaa atcagccgta tcgagaacaa gctggcgaat gaaggctttg
    16021 tcgcccgcgc accggaagcg gtcatcgcga aagagcgtga gaagctggaa ggctatgcgg
    16081 aagcgaaagc gaaactgatt gaacagcagg ctgttatcgc cgcgctgtaa tcaaaacaca
    16141 gtgatgaaaa cgaaggccgg agcatgctcc ggcctttttt atctcttaca gaaatggtaa
    16201 agaaggcata attccccgtg aaatactggc taagaatcct ttatctggtt gttcttcttt
    16261 attgattaaa ggaaggtcat ccaaatcgcc cagcaaatac gtattttttg cctgccagtc
    16321 aatcatccat attttcagca gtggataagc cagaccgccc gtaatcccgg atatcaccac
    16381 caacgcacac atgcgataaa gcataccgtg gtatgttaaa gttgagcgaa aacgaatacg
    16441 cccatcattc agtgacaggt tgctcataaa atgatttcgc aacgacaccg ttaaataact
    16501 ggtgctaaca gcaatcccaa aataatagat taactgcgcg attatcattt tacgttgcat
    16561 ttccataaat tgctgtaaat tctcaatatc atcatttgca tacccagaac tatcatactc
    16621 atttaatatt tgatcgaaga taatataacc agcaacagcg agaaaaggta ataaagccaa
    16681 aaatgcgaga atggcatatt ttatacaata tgtagtatct aatttcacct tgaaacgatg
    16741 tataccgaaa ctggtattac tccagagaaa actcattacc agactatata aagtgccatt
    16801 aaaaatacca atgaaaacaa tacaaactat tgtcatcaga accacagata taataacgct
    16861 acttgaacta ttggcatgta gcatctttgt cgagataaag aaaacagtcc ccatcccaag
    16921 ggccattaaa atcggcaaga aaaaggtcac ccaccagaac cctttcatag agcaattaaa
    16981 actaaatctt acgccgttga gactggtcat caaggcctga taacgcagtc cttttgctgc
    17041 cataaatata agcaaaacca acagtgacaa agtcaaaaca cagccaataa gcggcatatc
    17101 tgctgacact gtcattaaga ttgcgaaata gaaaaaaaca aaaacaagac aactaacaaa
    17161 aacattccca ccggtaatcc cataagaaaa tcgttgtccg ttaacttcca tattagcata
    17221 gagataacgc ttacatttca ttaatgccca tggtaaatag atacctagcg ttataatcgt
    17281 taacaacaca ttcaccacac aaataagaaa atatctctca cctgtaccat gaaaaacaaa
    17341 cgaatgggac ggaacatcca tatcattaat aacttgagcc ataaaagttc cctgaattaa
    17401 aaaatccatt gatataattt atatatacag acagtaaaat gttctgcc
//
LOCUS       DAJTZL010000083        17421 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.083, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000083 DAJTZL010000000
VERSION     DAJTZL010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17421
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.083"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(76..807)
                     /gene="rlmB"
                     /locus_tag="OO544_002378"
     CDS             complement(76..807)
                     /gene="rlmB"
                     /locus_tag="OO544_002378"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HCR6767958.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(987..3428)
                     /gene="rnr"
                     /locus_tag="OO544_002379"
     CDS             complement(987..3428)
                     /gene="rnr"
                     /locus_tag="OO544_002379"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HCR6767959.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYVPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3467..3892)
                     /gene="nsrR"
                     /locus_tag="OO544_002380"
     CDS             complement(3467..3892)
                     /gene="nsrR"
                     /locus_tag="OO544_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HCR6767960.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(4097..5395)
                     /gene="purA"
                     /locus_tag="OO544_002381"
     CDS             complement(4097..5395)
                     /gene="purA"
                     /locus_tag="OO544_002381"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HCR6767961.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVATTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5499..5696)
                     /locus_tag="OO544_002382"
     CDS             complement(5499..5696)
                     /locus_tag="OO544_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HCR6767962.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5778..6782)
                     /gene="hflC"
                     /locus_tag="OO544_002383"
     CDS             complement(5778..6782)
                     /gene="hflC"
                     /locus_tag="OO544_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HCR6767963.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6785..8044)
                     /gene="hflK"
                     /locus_tag="OO544_002384"
     CDS             complement(6785..8044)
                     /gene="hflK"
                     /locus_tag="OO544_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HCR6767964.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8130..9410)
                     /gene="hflX"
                     /locus_tag="OO544_002385"
     CDS             complement(8130..9410)
                     /gene="hflX"
                     /locus_tag="OO544_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HCR6767965.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9487..9795)
                     /gene="hfq"
                     /locus_tag="OO544_002386"
     CDS             complement(9487..9795)
                     /gene="hfq"
                     /locus_tag="OO544_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HCR6767966.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9881..10831)
                     /gene="miaA"
                     /locus_tag="OO544_002387"
     CDS             complement(9881..10831)
                     /gene="miaA"
                     /locus_tag="OO544_002387"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HCR6767967.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10824..12671)
                     /gene="mutL"
                     /locus_tag="OO544_002388"
     CDS             complement(10824..12671)
                     /gene="mutL"
                     /locus_tag="OO544_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HCR6767968.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMNVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRSIPENRVAAGRNHFAEPAAREPVAPRYTPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKLKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLALPVAERWLRQVQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12681..14018)
                     /gene="amiB"
                     /locus_tag="OO544_002389"
     CDS             complement(12681..14018)
                     /gene="amiB"
                     /locus_tag="OO544_002389"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HCR6767969.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(14037..14498)
                     /gene="tsaE"
                     /locus_tag="OO544_002390"
     CDS             complement(14037..14498)
                     /gene="tsaE"
                     /locus_tag="OO544_002390"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HCR6767970.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14470..16017)
                     /gene="nnr"
                     /locus_tag="OO544_002391"
     CDS             complement(14470..16017)
                     /gene="nnr"
                     /locus_tag="OO544_002391"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HCR6767971.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            16016..17155
                     /gene="queG"
                     /locus_tag="OO544_002392"
     CDS             16016..17155
                     /gene="queG"
                     /locus_tag="OO544_002392"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HCR6767972.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCIACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAQ
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILAALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17138..17191)
                     /locus_tag="OO544_002393"
     CDS             complement(17138..17191)
                     /locus_tag="OO544_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6767973.1"
                     /translation="MRFYFYSQAVDESGVTR"
BASE COUNT         3951 a         4975 c         4369 g         4126 t
ORIGIN      
        1 tcaccgtaat tatcttacca gctatagaca aaaaaaacca tccaaatctg gatggctttt
       61 cataattcag acgaactagc tgcgctgacg aaccgcttca aataagcaaa ttccggttgc
      121 aaccgaaacg ttcagggaag aaacacttcc tgccattggg atgctgatca actcatcgca
      181 atgttcacgg gtcaggcgac gcataccttc accttccgcg cccatcacca gcgccaagcg
      241 tccggtcatt ttgctctgat agagtgtatg atccgcctcg cctgccgtac cgacgatcca
      301 gatattctct tcctgcaaca tacgcatggt gcgcgcaagg ttagtcaccc gaatcagtgg
      361 aacgctttct gccgcgccgc aggctacttt tttcgccgta gcgttgagct gtgcggagcg
      421 atctttcggc acaatcaccg cgtgaacgcc agcagcgtcc gcgctacgca ggcacgcgcc
      481 gaggttgtgc ggatcggtta caccgtcgag gatcagcagg aacggttgat cgagcgaagc
      541 gatcagatcc ggcagatcgt tttcctgata ctgacgtcct ggcttcacgc gggcgataat
      601 gccctgatgc acggcaccgt cgcttttctc gtcgagatat tggcggtttg ccaactggat
      661 aaccacgccc tgagactcaa gggcgtgaat cagcggtaac agacgtttat cttcacggcc
      721 ttttaaaata aagacttcct gaaaacgttc aggggcgcgc tccagcaggg cctgcactgc
      781 gtggatgccg taaatcattt cgctcattaa tgtactcgtt gtttacgttt tgcctgatgc
      841 actacgttta tccggcctac atgatctctg caatatattg aatttgcgtg attatgtagg
      901 ccggataagg cgttcacgcc gcatccggca tgaacaaagc gcattttgtc agcaatctaa
      961 ccctcttctt ttaaagaggg tattgatcac tctgccactt ttttcttcgc cgcacgcttc
     1021 gctttggtcg ctgcggctat tttctgcgtt ttcgccgatg gctttttcgc ttttctcgcg
     1081 tctttcttcg ccgctttcgg cttcgttttt ttctcaccgc ggaaggcgct gtctggctca
     1141 aagtttacct ttttaccgac ctgacgacgc ttgccgcctt ttttgcctgc atcgcctttt
     1201 ttcgctttct cgcgcgccgt tttaccgacg ttgcgcggtg cgcgttcact ggagatcaga
     1261 ctaaagtcga ttttgcgctc gtccatatta accgcttcga cgcgaacttc cacgcgatcg
     1321 cccaggcgat aagtctggcc gctggattcc cccatcaggc gttgccctac ctggtcaaag
     1381 cgatagtagt cattgtccag cgaagagaca tggaccagac catcaatgaa caagtcgtcc
     1441 agacggacga agaagccaaa gccagtgacg ctggaaatta cgcctttaaa gacgttacct
     1501 acctggtcga gcatgaagtc acacttcagc cagtcagcga catcgcgcgt tgcttcgtcg
     1561 gcacgacgtt ccgccatcga acagtgctga cccagttgca acatctcttc catcgaataa
     1621 tggtagccgc cggtttcagt ggtgttgccc tgatgcccct gctctttcgc cagcagatac
     1681 ttaatggcgc ggtgcagcgt caggtcagga taacgacgaa tcggcgaagt aaagtgcgca
     1741 taggactgca atgccaggcc aaagtgacca cggttttctg gatcgtaaat cgcctgtttc
     1801 atcgagcgca gcagcatggt ttgcagcatt tctgcatcag gacggtcggc aaccgactcc
     1861 agcagttccg cgtagtcacg cggttccggc ttgttaccac ccggcagctc cagccccagc
     1921 tccgccagca ctgaacggaa agaggtaatc gcttcggtgc tcggcttgtc gtgaatacgg
     1981 aacagtgccg gttctttcgc tttctcaacg aaacgcgccg ccgagatatt cgccagaatc
     2041 atgcactctt caattaattt gtgcgcgtcg ttacgctggg tctgttcgat acgttcaata
     2101 cggcgttcag cgttgaaaat gaacttcgct tcttcgctct caaatgagat cccaccgcgt
     2161 tcttcacggg ctttatccag cactttatag aggttatgca actcttcgag atgcttaacc
     2221 agcgggacgt actgctcgcg cagatcctga tcgccctgca gaatatgcca gactttggtg
     2281 taggtcagac gcgcgtgaga gctcatcacc gcttcgtaga atttgtagcc cgtcaggcgg
     2341 cctttcgacg aaaccgtcat ctcgcacacc atacacaggc ggtctacctg cgggttgagc
     2401 gaacacaggc cgttagagag cacttccggc agcatcggga taacctgcga agggaagtac
     2461 accgacgtgc cacggttacg cgcttctctg tccagcggcg ttggcggacg cacatagtag
     2521 ctgacgtcgg caatcgcgac ccataaacgc cagccgccgc cgcgtttttt ctcgcagtaa
     2581 actgcatcgt caaagtcacg ggcgtcttcg ccatcaatgg tgaccagcgg taaatcgcgc
     2641 aaatcgacac ggcccgcttt tgcttcttcc ggcacttctt ctttcagccc cgcaacctgt
     2701 tgctcaacag cctgcggcca gatatacgga atttcatggg tacgcagagc gatatcaacc
     2761 gccatgccgg tgcccatatt gtcgcccagc acttcgacga ttttacccac cgctttggtg
     2821 cggcgagtcg gacgctgagt cagttcgact acgaccacaa agcccatccg cgcgcccatg
     2881 atctgatcgg gcgggattaa gatatcgaag ctcagacggc tatcgtcagg aaccacaaag
     2941 ccgacgcccg cttcggtaaa gtagcgacca acaatctggc tggtttttgg caccagtacg
     3001 cggacaatac gcgcttcacg acgaccttta cggtcagcac ccagcggctg cgccagcacc
     3061 tgatcgccat gaatacaggt tttcatctgc tcgctggaga gatacaaatc atctttacgc
     3121 ccttcaaccc gcagaaagcc gtagccatca cggtggccaa taacggtacc tttcaccagg
     3181 tcgaggcgtt ccggcagcgc atagcactga cgacgagtga agaccagttg accatcgcgc
     3241 tccatcgcgc gcaggcggcg acgcaggcct tcaagctgct cttcgccttc aatgtgcagt
     3301 tctaccgcca gctcatcacg gctggccggt ttttcacgtt tggttaaatg ttcgaggata
     3361 aattcccgac tagggatggg attcgcgtat ttttcagctt cgcgttcctg gaaaggatct
     3421 tgtgacatct cggttcctcc gttgtcatct ctgatgaaga ttttcgtcac tccaccagca
     3481 ataatttata aagcggttga ttctcttcaa ccaaatcggc aagcgtgtag ttatccagtt
     3541 ccgtaagaaa actttgcacg gccttagaaa gcgcctgttt caaccgacag gcaggtgtaa
     3601 tgtggcaaaa ctcactgctg caattcacca gcgataaggg ctccagctcg cgcaccacat
     3661 caccaatacg tatcgcactc gccggtttac ccaggcgaat gccgccattt tttccacgaa
     3721 cagcagtcac gtagccggca cgactaagtt gattgattat tttgaccata tgattacggg
     3781 agacgccgta gacgtcagtc acttcagaaa tactggtcat ccgcccttct ggcaatgacg
     3841 ccatgtagat cagcgcacgt aatccgtaat cagtgaaact cgttaactgc acatcaacct
     3901 caaaagggaa atcgggaaaa aatacattta tattgatgat aaaccagcca caagctgtgt
     3961 cgctaattta tttcagttcg gggaaggaaa aaagcgagga tttaacactg tgtcggatag
     4021 cgtaatggct taaccgacac agcaatgtat cacactgtta gcccggcagg caaaatatct
     4081 gccaggcgta ccagaattac gcgtcgaacg gatcgcgcag aatcatggtt tcagtacgat
     4141 ccggaccggt agagatgata tcgatcggca caccggtcag ctcttcaata cgcttgatgt
     4201 agttcagcgc cgcctgcggc aggccgctac gatctttcac gccgaaagtg gattcagacc
     4261 agcccggcat ggtttcgtaa atcggctcta cacctttcca gtcgtcagct gccagcggag
     4321 tggtagccac ttcgcgacca tccggcatac ggtaagccac gcagagttta acctctttca
     4381 ggccatccag aacgtccagc ttggtcaggc aaaagccaga cagggagttc agctgtaccg
     4441 cacgacgaac ggcaacggtg tccagccagc cggtacgacg acgacgaccc gtagttgcgc
     4501 cgaattcgtt accctgcttg cagaggaact cgccagtttc atcaaacagt tcggtcggga
     4561 acggacctgc acctacacga gttgagtaag ctttgaggat acccagaaca taatcaacat
     4621 aacgcgggcc caggccggaa ccggtcgcca cgccaccagc agtggtgttg gaagaagtta
     4681 cgtacggata agtaccgtgg tcgatatcca gcagcgtacc ctgcgcacct tcaaacatga
     4741 cgaaatcgcc acgctgacgc gcctggtcga gcaggtcaga aacgtcaacc accatagaag
     4801 tcaggatgtc ggcaacagcc atcgtatcat ccagaacttt ctggtaatca accgcttcag
     4861 ctttgtagta gttaaccaac tggaagttgt gatattccat cacttctttc agtttttcag
     4921 cgaaggtttc tttgtcgaaa aggtcgccaa cacgcagacc gcgacgtgct actttatctt
     4981 cataagcagg cccgatacca cgaccggtgg tgccgatcgc tttcgcgcca cgcgctttct
     5041 cacgcgcgtt atccagcgca acgtgataat caaggatcag cggacatgct tcagacagca
     5101 gcagacgctc acgaacgggg atgccacggt cttccagttc tttcatctct ttcatcagcg
     5161 cggccggaga cagcacaaca ccgttaccga tgatgctggt tacattctcg cggagaatac
     5221 ctgatggaat aagatggaga acggtttttt caccgttgat tacgagagta tggcctgcgt
     5281 tgtgaccgcc ctggtagcgt acaacatatt tagcccgttc agtcagaaga tcgacgatct
     5341 tacctttacc ttcgtcaccc cattgggtgc ccagtacgac gacgttgtta cccatttttc
     5401 aaaatcaccg tttgcttaaa aatggattct accatcgctt tttcagagtt acagcacttt
     5461 ttgcactcaa aaatgacctc gatcagtctg ttttttgttc agccaatcgt tttcctcaac
     5521 atgtagtaga ccacaacgcc cgcaaccaca agtccaccgc caaaacgacg taaaatatta
     5581 tcgggcaaat tggtcatcgc agagatcatc ttcttccatg ccttcgggta aagcatcggc
     5641 cctaaacctt ccagtaccaa aaccagggca agcgccagcc agattgtcga attcattcat
     5701 tatccttata gaaaaagaaa accaccgaca tccctgagga tgcggtggct ttattgacct
     5761 gtaccgcagt cgttatatta acgcgttgcg gaagtcggcg tcttcatgta gcggaagaaa
     5821 tcgctatccg ggctcatgac catcacgtcc tgattgccag agaagctgtt ctcataagca
     5881 cgcaggctac ggatgaatgc gtagaagtcc ggatctttac tgaatgcatc agcaaacagt
     5941 ttggctgctt cggcatcgcc ttcaccacgc atgatgcggc cctgacgctc agcttctgcc
     6001 agcgttctgg tcacttcata gtcggcagtc gcgcgcagtt tttccgcttc ttcctgacct
     6061 tgtgaacggt gacgacgcgc taccgcttca cgctcggcgc gcatacggtt gtagatcgct
     6121 tcagacactt cggtcggcag gttgatctgc ttgatacgca catcgacaac ttcaataccc
     6181 agcgccgcca tactgttcgg gttgatgacc ggaactttgc ccttcgtctc agccgttacg
     6241 cgctctgccg cttcggcaat ggcgttatct gccgccgggg tagtaacttc atcttctgta
     6301 cccgcagaac cggagttcag cgcgtcacgt acttcgaggg tcagacgacc acgggaatcg
     6361 gtgacgatgt ctttcacgtc caggcgacca atttcagaac gcagacggtc agagaactta
     6421 cgtttcaaca gcacttccgc ttgcgaaatg tcgccaccac ccgttgccag gtagtaacgg
     6481 ctgaaatcgc tgatgcgcca tttgatgtaa gaatcgacga tcaggtcttt cttctctttg
     6541 gtcacaaagc ggtcggcctg gttgtccatg gtctgaatac gtgcgtcgag cattttcacc
     6601 gtttcaatga acggtatctt gaaatgcaga cccggctcat aaaccagagg tttgttgtca
     6661 tcgtcacgca gtaccttacc aaaacgcagc gtaataccgc gctcaccttc tttgacgaca
     6721 aagacagaca tgtaaagcac taccagcacg atgatgataa tcgcgataac tgacttacgc
     6781 atcgttattc cccctgacgc tggtagtcgt tacgctgcgc gttggcgcgg cgttggtcca
     6841 taatatcgcc ctgactggtg gacgacgtgt tgcttgctcc actggttgtg gaggaagagg
     6901 ctggcggcag acgcagcaga ttgctggcac cgttatcgct cttcgccgca ggggcgttac
     6961 cgcctttcag catctggtct aacggcagaa ccatcaggtt gccaccttta tcgttaacca
     7021 gcactttgcg ggtgttaccc aacacttttt ccatcgtctc gatatacaga cgctcgcgag
     7081 taatttccgg cgcggcttta tattccggca gaagtttagc aaagcgcgcc acttcaccct
     7141 gagcttccag gatggtctgg gccttgtacg cacgcgcctc ttcgaggata cgttgcgcct
     7201 gaccgttcgc acgcggctga acttcgttgg tatacgcttc tgcttcacga atgtattgct
     7261 gttcgttttc acgcgcggca atcgcatcgt caaacgccgc ttttacttct tccggcggac
     7321 gagctgcctg gaagttgacg tccagcagag tgatacccat gtcatacgga cggatagtct
     7381 cttccagttc gcgctgagta tcgctacgaa tcacggtacg accttccgtc agaatgcggt
     7441 ccatggtgta tttaccgata actccacgca gggcgctgtc ggtagcctga cgcaggctgt
     7501 catccgggct ggtcacgcta tacagatatt tttccggatt ggtgacgcgg tactgcacgt
     7561 tcatctcaac gcgcactacg ttctcgtccg acgtcagcat cacaccagaa gcggccagtt
     7621 cacgcacggc ttccacgttc accggtttga cttcgtcgat aaacgtcggt ttccagttca
     7681 gacccggctc aaccagatgg ctgaatttac caaagcgtgt taccacgccg cgttcggctt
     7741 ctttaatggt atagaaacca ctggccgccc agataatgac aatcgctgcc gctgcgatgg
     7801 taacgacacg accgccaagc tgcgggcgcg ggccttgcga tgaactgcca ccgccagatc
     7861 cggtgccttt accgccgccc agaccaccga gctttttgct cagtttgcgg aagatatcat
     7921 ctaaatcagg tggcccttga tcgcgaccgc ctttgtttcc atttccctca gagttgccgc
     7981 caggtttgct gcttccccac gggtcgcggt cttgtccgtt attaccgggc tgattccacg
     8041 ccatgtttgt gctccatatt tgttatgcgg tgatccctgt tgtctttcga cgccagagga
     8101 tatgactcca cgcttcagac gctacgccgt tagatcaggt aatcgatcaa cgccggttct
     8161 tgtttacaga ggcgacgcca gtcaacgatc ggcatacgaa cttgcagact tacgctgccg
     8221 tcctcctcca tccactcttt ttctattgcc tgaagctgat aaaaacgact tctcagacgt
     8281 ccttcctgcg gcggcaaacg caatgtatgc tgcgccacct cgccggaaag ccgctccgtc
     8341 aaagcctgaa aaagctgtgg tatccccgct ccggtctgtg cggaaagcca gacacggatc
     8401 ggtttgttct cttcgtcccg atcaatacgc ggttcgaaat cttccagcat atcgatcttg
     8461 ttcatcacca gcagggttgg gatctcgtga gcgtcgatct cttcaagaac cgtattcacc
     8521 gcttcgatgt tttcttgtac acgtacatcc gccgcatcaa tgacgtgcag cagtaatgtg
     8581 gcttgccgcg tctcttgtaa cgtggcttta aatgctgcca ccagatcgtg cggcaggtgg
     8641 cgaataaacc ctacggtatc tgcaagtacg gtttcaccga catccgcgac gtcaatacgc
     8701 cgcaacgtcg ggtcgagggt ggcaaacaac tggtctgccg cgtagacccg cgcttcggtg
     8761 atgcgattga aaagggtaga tttaccggcg ttggtatatc ccaccagcga aacagtagga
     8821 acgtcggctt tgatacgcga ttgccgcccc tgctcacgct gcttttcaac tctttctagg
     8881 cgcgactgta tctgcacgat gcgattacgc aacaaacgac ggtcggtttc gagctgggtt
     8941 tcacccggac cacgcaaacc tatcccgcct ttctgtcttt caaggtgggt ccagccacgc
     9001 accaggcgcg tagccagatg gcgcagctgc gctagctcaa cctgcaactt accctcatgg
     9061 gtacgcgcac gttgggcgaa aatatctaaa ataaggccgg tgcggtcgat aacacgacac
     9121 tcgcacaaac gctccaggtt acgctcttgc gccgggctca gggcatggtc aaaaagaacg
     9181 accgaagcac ccgtcgcttt gacagcttcc gcaatttcaa ctgctttacc ttcacctaca
     9241 aaatactttg ggtgcggcgc tttacggcta ccggtaatca cctgcaatgc ttcgacaccg
     9301 gcggaagaga ccagagattc aaactcctgg aggtcttcca tatctttgtc ttgcgtaaaa
     9361 tagatgtgta ccagtaccgc ctgctcacca gcatcataac ggtcaaacaa gcgtataacc
     9421 ctctaaatag atcagcgggg aacgcaggat cgctggctcc ccgtgtaaaa aaaacagccc
     9481 gaaaccttat tcggtttctt cgctgtcctg ttgcgcggaa gtattctgcg cgctgctacc
     9541 atgatggtag ttactgctgg taccgccacc ggcgttgtta ctgtgatgag aaaccgggcg
     9601 agacgggaca acagtagaaa tcgcgtgctt gtaaaccatc tggctgaccg tgtttttcaa
     9661 caggatcacg aactgatcaa aagactcgat ttgcccttgc agcttaatac cattcaccaa
     9721 ataaatagaa actggaacac gttcccgacg cagtgcgttc aggaacggat cttgtaaaga
     9781 ttgcccctta gccattctct cttttcctta tatgcttatt tgtactttga acctttcgat
     9841 tctgaaaaaa ttgcgcacga tacgtctcaa ttgtacacat tcagcctgcg atagcaccaa
     9901 caacctgtaa tacttcgtca cgcgcctgtt ctggtttttc actgtcaagc cagtgaaccc
     9961 cttcccaacc acgcagccag gttatctgcc gcttcgccaa ctgtctcgtg gcgcaaacac
    10021 ctcgataaac catttcatcg tatgagattt cgccttcaag gtaagaccac atctggcgat
    10081 aacccacgca acgaatggaa ggcaagtccg tatgcaaatc tcctcgggca aaaagcgccc
    10141 ggacttctgc ttcaaaacct gaagccaaca tctgatgaaa acgctgctca atgcgttgat
    10201 ggagcagttc acggctcgcc ggggcgatgg cgaactgatg cacctgatac ggtagagcgt
    10261 ctcctgacgt ttgcgtcagt tccgttaaag ttttacccga aatgaaaaaa acttccagtg
    10321 cccgggaaag cctttgtgga tcatttggat gaatccttgc tgccgcaacc ggatctacct
    10381 cctgaagttg acgatgcaat gactcccaac cttgctctgc cgcctgttgc tcaattctgg
    10441 cccgcacttc cgggtctgcc gacggtagcg gcgacaaccc ttccagcaat gccttgaaat
    10501 acaacatcgt accgcccact aacagtggga tccgccccgc cgcggtgata tcggccattt
    10561 ccgccagcgc atcgcggcga aaatcagcag ccgagtaagc ctgcgacgga tcgcgaatat
    10621 ccagcaatcg gtgcggcgcg gcgagtaact cttcagcgtt cggcttcgcc gtcccgatat
    10681 ccatcccttt gtaaataagg gcagaatcaa cgcttatcaa ctctactggt aaaattttac
    10741 gcagctcaat ggctaacgcc gttttaccgg aggccgtcgg ccccatcaaa aaaatcgcct
    10801 taggcaggct cgccttactg atatcactca tctttcaggg cttttatcgc cggatgtaaa
    10861 tcaacagatt gtaacagacc acccggcggc gttttcacaa gttgcggaca taaccgttcc
    10921 acgtccgcca gcagggttat ggcctgtgcc attgaccact gcgcatgttc gctcatcaga
    10981 tttcgtgcaa tccactgcgc aatattgcca ggttcgaata cggactgctt cgccaggtag
    11041 cctatcagtt caggaatcaa gatttgtaaa ttttgttggc gtaagggtaa aggcacggcc
    11101 ctgatggtca catgctgtgc atctgactgg aaatcaatac ccaattccgc cagggcagac
    11161 tgcgcttttt ctaatgccga tttttcttcg ccagaaactt ttagccgcaa cggaataagc
    11221 agcggctgag cgcaaactgg cgcttcaccc ggcgtcagtt gtacctgacg cagccaacgt
    11281 tctgccactg gcaaggctaa aagtgaaatg ttgccgtcgc gctccagcaa cgcacagtcg
    11341 gaatggacga tagtcagtac ccgaccaaaa ctctgactgt tcgccgcaag tgcaggttcc
    11401 tgcggttccg gcgcttttaa tttttgcatc ggcgcgggcg tttgcaaaag ctggcgatac
    11461 acttcacctt gctgtttctg gtagcctggc tgcgcattcg gccagggggc agccggacga
    11521 ctgcctgatg ctggcgcagg agtgtagcgc ggagctaccg gctcacgagc tgccggttct
    11581 gcaaagtgat tgcgccccgc cgccacgcgg ttttccggaa tggaacgcgg tgcaggttgg
    11641 ggttcatcgt ccagcggtag cggcgtttcc agttgctgtt gtagcacgct cagcacgccc
    11701 tgatagataa aatcatgcac cagacgcgac tgatggaaac gcacttcgtg tttggcgggg
    11761 tgcacgttga cgtccacctg atgcggatcg atctccagat acagcacaaa tgccggttgc
    11821 tgatcggccc ccagtttgtc ttcgcaggcc tggcggatcg cgtgattgat caggcgatcg
    11881 cgcatcatgc gaccgttcac gtagcaatac tgaatttctg ccagtgcggg cgtggtgtga
    11941 tttggatcgg ccacccagcc gcgtagcgtg agatcgccgt gttgccattc aatcgccagc
    12001 gcttgttcaa gaaaagcggt gccgcaaatc gcgcctaagc gccgttcttt ttgcccgcct
    12061 tccggcactg cgcggtactg acgcacaatt ttaccgttat gcgacaggtt gatcgtgacg
    12121 tcgaaacgcg ccagcgcaat gcggcggatg atctcatcaa tgtggttaaa ttcggttttc
    12181 tcggtgcgca ggaatttgcg ccgcgccggg gtgttgtaga acagatccag cacctccagc
    12241 gtcgtcccca caggatgcgc cgccggtttt accgtcacgt tcatatcgcg cccttcggca
    12301 taggcctgcc aggcttcctg ctgttctgcg gtgcgtgaag tgagcgtcag gcgggaaacc
    12361 gaactgatac tcgccagcgc ctcaccgcga aagcccaggc tgataatggc ttcgagatcg
    12421 tccagagagg cgattttact ggtggcatga cgagccagcg ccagcgccag ctcatctttt
    12481 ttgataccgc agccgttatc acgaatgcgg ataagtttcg ccccaccgcg ttcgatatca
    12541 atatcgatac gcgtcgcacc tgcatcgagg ctgttttcca ccagttcttt gactaccgac
    12601 gcaggtcgct cgaccacctc acctgcggca atctggttcg ccagttgtgg cggtaagacc
    12661 tgaattggca tcaatcgtcc ttagtttggc agcgtgcgat ctggcgtcgt caccgtactg
    12721 gcagtttgtg ccgttgcccc ctgcggcgca gattgcatcg gatgcgcaag gaaataattg
    12781 cgcaggcctt tgtaaatggc ttctgccagc tgttgttggt aatcgtcgct cgccagcaaa
    12841 cgttcttcgc tgttgttgct gataaaaccg gtttcgacca gtactgacgg gatatccggc
    12901 gaacgcagaa cgccgagact cgcgtgttct ggtcggcgtt tatgtatttc gccaatgcgt
    12961 tgcaactgac tgatcatact ggtcgctaca tcatacccta cccgctggga atgaccgaac
    13021 tgtaaatcca gcaccgcctg gcttaaatag gggtcagact gactgttcgc cagcacatcg
    13081 cccgccccac ccagcagctc cgactgtttc tcgtgctgct ccagccagct ggccatttcg
    13141 ctgttggcgc gacggttaga gagcacccat acggaagcgc cagtcgcact gcggttcggt
    13201 gcggcatcag cgtgaatcga cacgaggaaa ttggcgtttt gcttacgtgc cacatcgctg
    13261 cgccccatca ccgagataaa gtaatccccg tcacgggtta aaacgccttt aaacatcgga
    13321 tcgtcattga gcaaagtgcg caatttacgc gcgatggcga tggtgacatt tttctcccgc
    13381 gtaccaccgg ggccgatagc gccagggtcc tgaccgccgt gtccggcatc aatagcgatg
    13441 ataattttat cgccagtgtt agccgtcgcg cgcgctgccg gacgcgttac cgtattactg
    13501 ctgataacac ccgtagtgcg gttactttcc gttttaaacg gattgcgcgc cggttcgctg
    13561 acgcgcggtg cgacaaccgc aggcgtttca acgcgtttcg caaccacggg cggtggagga
    13621 ggcggtggcg gcacatcggc gttaatcgta aagacgacag tgtaattgct gccattctgc
    13681 cgcttcaccg cttcggtttt accgttttcg gtaagatcga ccaccagccg tagcgtttgt
    13741 gcatctttag gcgttccaga gcgaatcgcc ttcaccagat tattgccgct gaacaacaac
    13801 ggcagtccct gaatcacgcc cgtttgtttg atatcgagcg ccacggtgcg tttgctttga
    13861 tggctaaacg cataatcagg atcgccaata aaactcaacg ttatccgcgc ctgttgatta
    13921 ccgttagaaa cctgaatatc agagagcgtc gcggcaccca ccggcgtgca cagcagcagc
    13981 agcgtcgcta ccaaccaatt tctgatgcga tacatcatcc cgccaccttt caaaggttaa
    14041 ccggctaaac gcgccagcaa caattcaccc gcagaggaaa ccgcactcac gcgcgcctca
    14101 cggccttgtg cctgataatc aatgtgtatt tcgacatccg ggtcaggaag aacacctgta
    14161 ccttgttgtg gccactccac caggcagatg gcatcgttgg caaaataatc gcggatcccc
    14221 ataaactcca gctcctcggg atcggcaagg cggtacaaat caaagtgata aaccattaaa
    14281 ttgtcgagcg tatagggttc gaccagcgta taagtggggc ttttgacatt accctgatga
    14341 cccagagctt gtaaaaaacc ccggctaaag gtggttttac ctgcgcctaa atcgccatac
    14401 agatagatta cggttgcgcc atcgcaggct ttcgctaccc gctcgcccag gtctaatgtt
    14461 gcctgctcat cagggagcgg aattactcga ttcatcatgg tttttatcag tcacttccgg
    14521 gttaacaata cgctgtagcg tggaaaagag atcggttgcc agcatcccgc gcgttccaaa
    14581 acgcgccgcc agtacgtcag ctgccgcacc gtgcgcgaca cagcccgcac aggcggcatc
    14641 atacggcgac agtttttgcc caagcaatgc gccaataata ccagagagca catcgcccat
    14701 gccgccgctc gccatgcctg catttccggc atcaataatg cctaaagcgt caggatgggc
    14761 ggcgaccacg gttccggcac ctttcagcac cgctacgccg ccataacgtt gtaccagacg
    14821 tttggcgcaa tgtaagcggt cactttcaat ttcagcgacg gaacagccta acaaccgtgc
    14881 ggcctcgcca ggatgcggcg tgatcacgcg attgtgacgc ttatcgggat tgattgccag
    14941 caggttcaat gcatcggcat cccacaacat cggtttgcga aaattctcaa ctttttgcag
    15001 tgcttttttc ccccactctt gctggcccag accgggacca atcaccacca catcggccca
    15061 ttccaggctt tcggtaagag agtccatcgt cagttcatgc accatcaatt ccggtcgtgc
    15121 agtcagcagc ggcgcaatgt tttcactgcg ggtcagtact cggactaaac cagcaccagc
    15181 acgcagcgcc gcttcccccg tcatacgaat agcccccgcc gtgccgtgat cgccaccgat
    15241 aatcaccagc cgcccgtgat cgcctttatg cgaagtcggg cgacgcggtt tcagccagtg
    15301 agaaagttgt tctgccgaaa accgctgaat tttcgtctcc tgacctgcca gccagctatc
    15361 cagccccagt gagtcaaaat gcagttgtcc tgtaacatcc cgcgcttttc cagtaagcaa
    15421 gcctggtttc agcgcaataa aagtgatggt gtgatcggcg ttgatcactg cgcctggcgt
    15481 agcgccggtt tcagccagca ggccggaagg gatatcaacc gccacaatcg gcgcaggatg
    15541 ggaattagcg tggtcgatta actggctaat ggattcgcgg ggcgcttgct gcaagccggt
    15601 accgagcagc gcatcaacaa tcagatctac cgattcgggc cagacaatat tcgaagcatg
    15661 gatctcgcca cccgcgttta accatgcttc gcgtgccagc gcggcctctt ccggcaacgg
    15721 tttgtcgctc tcctgggcca acaacgtgac ctcaatgccg accgctttgg ccagtcgcgc
    15781 gaccacgtag ccatcgccgc cgttattacc atgaccgcac agcaccagcc agtggcgggc
    15841 gtcaggatac gccgaacgac acacctggaa tgcggcctcg ccagcgcgaa gcatcagctc
    15901 atagagtgtg agccccagca catctgccgc ctcgcgttct ccgcggcgga tatcgtcggc
    15961 gtaccagacg gtgtgtggta tacttacggg gtttttcttc attgtatggt ccgtcatgtc
    16021 agagcccctc gatctcaatc agttagcgca aaaaattaaa cagtgggggc tggaactggg
    16081 ctttcagcag gtaggtatta ccgataccga tctcagcgag tccgagccca aactgcaagc
    16141 atggctggac aaacaatacc acggcgaaat ggactggatg gcacgtcacg gtatgctgcg
    16201 cgctcgcccc catgagttat taccaggtac gctgcgtgta atcagcgtgc ggatgaatta
    16261 ccttcctgct aacgccgcat ttgccagcac gctgaaaaac cccaaactcg gctatgttag
    16321 ccgttatgcg ctgggccgtg actatcacaa acttctgcgc aaccgactca aaaagctggg
    16381 cgagatgatt cagcaacatt gtgtttcgct gaattttaga ccgtttgtcg attctgcgcc
    16441 tattctcgag cgcccgttag ctgaaaaagc tgggctcggc tggacaggta agcactcact
    16501 tatcctcaat cgcgaggccg gttctttctt ctttttaggc gaattgctgg tcgatattcc
    16561 gctgcccgtg gatcaaccag tcgaggaagg atgcggtaaa tgcatagcct gtatgacgat
    16621 ttgcccgacc ggtgccatcg tcgagccata taccgtcgat gctcgccgct gtatctctta
    16681 tctcaccatc gaacttgaag gggcgatccc ggaagagttg cgaccgttaa tgggaaaccg
    16741 tatttacggt tgcgatgact gccagcttat ctgcccgtgg aatcgctatt cgcaactcac
    16801 tacagaagac gatttcagcc cgcgtaagcc gctacacgca caggaactca ttgagttatt
    16861 tgcctggagc gaagagaagt ttttaaaagt cacggaagga tcggcgattc gccgtatagg
    16921 tcacctgcgc tggctgcgta atatcgccgt ggcattaggc aatgcgccct gggatgaaac
    16981 gattctggcg gcgctggaga gtcgcaaagg tgagcaccca cttcttgatg agcacatagc
    17041 gtgggcgatt gcgcagcaaa tcgagagacg aaatgcgtgc atagtcgaag tgcaattgcc
    17101 gaaaaaacag cgtctggttc gggtgattga aaaagggtta ccgcgtgacg cctgattcat
    17161 ccacagcttg tgaataaaaa taaaaacgca ttgcaattca agacgcataa aatcgtcaag
    17221 tgatcgactc gacaaaacgg aatcttgatt tttaacttat ttatcaatag gttaaaaaaa
    17281 taccattcac cgtgcgaagt ttctttgaaa ttaagattac agcctgagga tgtggataac
    17341 tctgtttaca agagtttgtc agagacaaca aaaaccagca ataacgcatc gcgtcgctgt
    17401 ggatatttta ttgagagaag a
//
LOCUS       DAJTZL010000084        17142 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.084, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000084 DAJTZL010000000
VERSION     DAJTZL010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17142
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.084"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            191..2038
                     /gene="ldtD"
                     /locus_tag="OO544_002394"
     CDS             191..2038
                     /gene="ldtD"
                     /locus_tag="OO544_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR6767974.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAVFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALATPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            2219..2767
                     /locus_tag="OO544_002395"
     CDS             2219..2767
                     /locus_tag="OO544_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HCR6767975.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2794..3441
                     /gene="gloC"
                     /locus_tag="OO544_002396"
     CDS             2794..3441
                     /gene="gloC"
                     /locus_tag="OO544_002396"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HCR6767976.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3663..4853)
                     /gene="aspC"
                     /locus_tag="OO544_002397"
     CDS             complement(3663..4853)
                     /gene="aspC"
                     /locus_tag="OO544_002397"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HCR6767977.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSSLINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(5038..6126)
                     /gene="ompF"
                     /locus_tag="OO544_002398"
     CDS             complement(5038..6126)
                     /gene="ompF"
                     /locus_tag="OO544_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HCR6767978.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVELVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
     gene            complement(6728..8128)
                     /gene="asnS"
                     /locus_tag="OO544_002399"
     CDS             complement(6728..8128)
                     /gene="asnS"
                     /locus_tag="OO544_002399"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HCR6767979.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(8299..9501)
                     /gene="pncB"
                     /locus_tag="OO544_002400"
     CDS             complement(8299..9501)
                     /gene="pncB"
                     /locus_tag="OO544_002400"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HCR6767980.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            9767..12379
                     /gene="pepN"
                     /locus_tag="OO544_002401"
     CDS             9767..12379
                     /gene="pepN"
                     /locus_tag="OO544_002401"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HCR6767981.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPEHFTLKIVNEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKTKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPIVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLEEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            complement(12586..13353)
                     /gene="ssuB"
                     /locus_tag="OO544_002402"
     CDS             complement(12586..13353)
                     /gene="ssuB"
                     /locus_tag="OO544_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HCR6767982.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQEDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(13350..14141)
                     /gene="ssuC"
                     /locus_tag="OO544_002403"
     CDS             complement(13350..14141)
                     /gene="ssuC"
                     /locus_tag="OO544_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HCR6767983.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVTAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARS
                     YGLSGIPLFIHVILPGALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            complement(14153..15300)
                     /gene="ssuD"
                     /locus_tag="OO544_002404"
                     /pseudo
     CDS             complement(14153..15300)
                     /gene="ssuD"
                     /locus_tag="OO544_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309045.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
     gene            complement(15297..16256)
                     /gene="ssuA"
                     /locus_tag="OO544_002405"
     CDS             complement(15297..16256)
                     /gene="ssuA"
                     /locus_tag="OO544_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HCR6767984.1"
                     /translation="MRNIIKLALAGLLSVSTFAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLIYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VSAEVAALQQQTADLFYENRLVSKKVDIRQRIWQPTHLEGKQL"
     gene            complement(16249..16824)
                     /gene="ssuE"
                     /locus_tag="OO544_002406"
     CDS             complement(16249..16824)
                     /gene="ssuE"
                     /locus_tag="OO544_002406"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HCR6767985.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSAMKAQELLHGVFADDSQVIDYHHKPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLLSLRGNVHA"
BASE COUNT         4298 a         4421 c         4349 g         4074 t
ORIGIN      
        1 ttacggcgcg cttttcttta aacttcttta cattaggtta tgtaaaaacg tatcggcgtt
       61 tatatactga agataagcct gatgagtaac aggcttgctc gtcatacttt cgtgagtatt
      121 ggcgttgtac aggcaagtcg taaaataaca gcctggctat tcagagtatg ataaaaacag
      181 ggggcaaggg atgttgctta atatgatgtg tggtcgtcgg ctgtcggcaa tcagtttgtg
      241 cctggccgta acattcgctc cactgttcaa tgcgcaggcc gatgagcctg aagtaatccc
      301 tggcgacagc ccggtggctg tcagtgaaca gggcgaggca ctgccgcagg cgcaagccac
      361 ggcaataatg gcggggatcc agccattgcc tgaaggtgcg gcagaaaaag cccgcacgca
      421 aatcgaatct caattacccg caggttacaa gccggtttat cttaaccagc ttcaactgtt
      481 gtatgccgca cgcgatatgc aacccatgtg ggaaaaccgt gatgctgtta aagccttcca
      541 gcaacagctg gcagaggtgg cgattgccgt tttccagccg cagtttaata aatgggtaga
      601 gttactgacc gatcctggtg ttaacgggat ggcacgcgac gtagtgctct ctgatgcgat
      661 gatgggctat ctccatttca ttgcaaatat tccggtcaaa ggcactcgct ggctatatag
      721 cagtaaacct tatgcacttg caacgccgcc gctctcggtg attaaccaat ggcagctggc
      781 gctggataaa ggtcaattgc ctacgtttgt tgcaggactg gcaccgcagc atccgcaata
      841 tgcggcgatg catgaatcgt tactggcctt actcagtgac accaaaccgt ggccccaact
      901 gaccggcaaa gcaacgttgc gcccagggca gtggagtaac gacgtaccgg cgttgcgcga
      961 aatattgcaa cgcacaggca tgttggacgg ggggccgaaa attactctac ctggcgatga
     1021 cacgccaact gacgcggtag tcagcccatc cgctgttact gttgaaacag cagaaactaa
     1081 gccgatggac aagcaaacga cgtctcgtag taaacctgcg cctgccgttc gcgccgccta
     1141 cgataatgaa ctggtggaag ccgttaaacg ttttcaggca tggcaaggat tgggggcaga
     1201 tggtgctatt ggcccggcaa cacgtgactg gttaaacgta acgcccgccc agcgtgctgg
     1261 cgtgttggct ctcaacatcc agcgattgcg cttgctgcca acagagcttt ctaccgggat
     1321 catggttaac attccggcct attcgctggt ctactatcag aacggcaatc aggtgctgga
     1381 ttcgcgagtc attgtcggtc gccccgatcg caaaacgccg atgatgagca gtgcccttaa
     1441 caacgtagtg gtaaacccgc cgtggaacgt acctccaact ctggcacgca aagatattct
     1501 gccaaaagtg cgcaacgatc cgggatatct cgaaagccat ggctatacgg tgatgcgcgg
     1561 ctggaacagc agagaagcga ttgacccatg gcaggttgac tggtctacaa tcacggcctc
     1621 gaatttaccg ttccgcttcc agcaggctcc aggcccacgg aactcgctgg ggcgctataa
     1681 attcaatatg ccgagttcag aggccattta tttgcatgac acgccgaacc acaatctgtt
     1741 caagcgtgat acacgcgcat tgagctcagg ctgtgtacga gtgaataaag cttccgatct
     1801 ggcgaatatg ctgttgcagg atgcaggctg gaatgacaaa cgtatttctg atgcgctgaa
     1861 gcagggtgat acacgttacg tcaatattcg gcagtcgatt ccggtgaatc tctactacct
     1921 gacggccttt gttggtgcag atggtcgtac ccagtatcgt acagatattt acaattatga
     1981 tctgcctgcg cgatccagct cgcaaatcgt atcgaaagcg gaacaattaa tcaggtaaat
     2041 gaagtagttc ggggaaatca gttgtcgtaa taattagcat gattgggggc gattctctgc
     2101 agcccccgtc actgctgggg ttgagtcatc ttgacgtctg ctttacgggc ggttaaggtg
     2161 cctcttgtgc gccagaagtg catataaacg ataacattga cctgtagact tgattatcat
     2221 ggacaaattc gacgctaatc gccgcaaatt gctggcgctt ggtggcgttg cactcggtgc
     2281 cgccatcctg ccgacccctg cgtttgcaac actctctacc ccacgcccgc gcattttgac
     2341 actcaataat cttcataccg gagagtcaat caaagcggag tttttcgatg gcagaggcta
     2401 tattcaggaa gaattggcaa aacttaacca ttttttccgc gattaccgcg cgaacaaaat
     2461 aaagtccatc gacccaggat tattcgacca gttgtatcgc ctgcaagggt tgttaggcac
     2521 gcgcaaaccg gtgcaactca tttccggtta tcgttctatt gataccaaca atgaactacg
     2581 cgcccgcagc cgtggagtag cgaagaaaag ctatcacact aaaggccagg cgatggattt
     2641 ccatattgaa ggtatcgcgt taagcaatat tcgcaaagcc gcgttatcta tgcgcgcagg
     2701 tggtgtagga tattatccac gtagtaactt tgtgcatatt gataccgggc cagcacggca
     2761 ctggtagtaa ttgcttaacg aaacaggggc agtatgaact atcgtattat tccggtcacc
     2821 gcattctccc agaactgttc attaatctgg tgtgaacaaa cccgtctggc cgcactggtc
     2881 gatcctggcg gcgatgcgga aaaaatcaaa caggaagttg atgacagcgg cctgacactg
     2941 atgcagatcc tgctgacgca tggtcatctg gaccacgttg gcgcagcggc ggaactggcg
     3001 caacattacg gcgtgccggt ttttggcccg gaaaaagaag atgagttctg gctgcaaggc
     3061 ttgcctgcgc aaagtcgtat gtttggtctg gaagagtgcc agccgctgac gccagatcgt
     3121 tggctgaacg aaggcgatac catcagcata gggaatgtga ctttacaggt gttacattgc
     3181 cctgggcata cgccgggtca tgtcgtgttt tttgatgatc gggcaaagct gctgatttct
     3241 ggcgatgtta ttttcaaagg cggagtaggg cgcagtgact tcccgcgtgg cgatcataat
     3301 caactgattt cttcaatcaa agataaattg ctgccactgg gggatgacgt gacatttatt
     3361 ccgggtcacg gaccattatc cacacttggt tatgaacgcc tgcataatcc cttcctgcaa
     3421 gacgaaatgc ccgtctggta aggcacataa aaaagcccgc ttttaatgcg ggcctggatt
     3481 tctggcaaag tgcgctttgt ttatgccgga tgcggcacga gcgccttatc cggcctacaa
     3541 aatcgtgcaa attcaaaata ttgcagggga cgcgtaggcc tgataagcgt agcgcatcag
     3601 gcaatgttgc gtttgtcatc agtctcagcc cgcttttcag cgggcttcat tgtttttaat
     3661 gcttacagca ctgccacaat cgcttcgcac agcggagcca tgttatctgg tgtcatcccg
     3721 gccacattta cgcgaccaga agcaaccgca tatacgccaa actcttcgcg cagacgcagc
     3781 acttgttctt ttgtcaggcc actgaaggag aacatgccgt tctgtttgat gataaagctg
     3841 aagtcgcggt ttgcgccttt ttcctgcagc gtattgacga acaactgacg catacgctga
     3901 atacgctggc gcatatcagt cagctcttgt tcccaaatcg cacgtaacgc atcgttgctc
     3961 aggatggtgg caacaacaga agcgccgtgt gctggtgggt tagagtagtt agcgcgaatc
     4021 gccgctttca tttggctgaa tgcgcgatca acggtttcac tgtcggcagc aaccagagta
     4081 caagcgccaa cacgctcgtt gtacaggcca aagtttttag agtaggaact ggcaacaatc
     4141 agctctttat gcatagccgc gaaagcgcgc agtccttcag catcttcttc cagaccacgg
     4201 gcaaaaccct ggtaagcgaa gtcaaacagc ggtaaccagc ctttctcaac ggagagttgt
     4261 gccagtgtct gccattgttc cagcgtaggg tcgataccgg ttgggttatg gcagcagcca
     4321 tggaacagca ctacgtcgcc agcctgagct tcattcaggc tgttaatcag tgcatcgaag
     4381 tcaagagtgt gattttccgc atcataataa gcgtattcac gaacttccag acctgcagag
     4441 ttaaagacgc tcttatggtt cggccagctt gggttgctca cccacactcg cttaacgctg
     4501 gtattttttg ccaggaaatc ggcagctacg cgtagtgcgc cagtgccccc cggagtctgt
     4561 gccgtgcgag cacgtttgtc attgatcagg gagctacctt taccaaacag cagttcctga
     4621 gtgcagcgac caaattcagg gatgccgtca atgccgaggt aatttttggt ggtttcattt
     4681 tcgagcagat actgttcagc ctttttcacg ctggtcagta ccggggtttt gcccgtctca
     4741 tctttataga caccaatccc gaggttaatt ttgccgggac gttcatcggc acgaaacaga
     4801 tcggccaggc ccagaatcgg gtcggcagga gcggcggtaa tgttctcaaa catgacgagg
     4861 ttccattatg gttacagaag ggaagtccgc tatcagggta acgggagatt tacaaaattc
     4921 caactattac tgatgaaaac gcaggctgtt tttgcaagac gtgagattgc tctggaaggt
     4981 ataaaaaaaa caggaccaaa gtcctgtttt ttcggcattt aacaaagagg tgtgctatta
     5041 gaactggtaa acgataccca cagcaacggt gtcgtctgaa cctacgccca gtttgttgtc
     5101 agaatcgatc tggttgatga tgtagtcaac ataggtggac atgtttttgt tgaagtagta
     5161 ggttgcgccc acttcaaagt agttcaccag ctcaacatca ccgatacctt ctacgtcttt
     5221 cgctttagat ttggtgtaag cgatggacgg acgcagaccg aaatcgaact ggtattgcgc
     5281 aactaacaga acgtcttgcg ttttgttggc gaagccgctg gtgtttgtaa atttattagt
     5341 gatcggcgta gcgttacggg tttcaccgta gttcgctgcc aggtagatgt tgttcgcgtc
     5401 gtacttcaga ccagtagccc actgttcagc ttttttaccg ttgccaagag gttgagcttc
     5461 ttgcaggttg gtacggtcag ctgcaccata agcaccaacg ataccaaagc cttcgtattc
     5521 gtagctgata gaaccgccaa caccgtcgcc gttagaacgg cgtgcagtgt cacgctcgtt
     5581 tttacccagg tactgaacag cgaagttcag gccatcaacc agaccaaaga agttggagtt
     5641 acgataggta gcaacgccgc caacacgacc aacgaagaag tcatcgctgt atgcagtatc
     5701 accaccaaat tctggcagca tatcggtgta acccagtgca tcataaacca caccgtagtt
     5761 acggccgtaa tcgaaagaac caacgtcagc gtatttaaga cccgcgaatg ccagacgcgt
     5821 tttgttacca gtttgagcgt cagcgccttc agagttgtta ccctggaagt tatattccca
     5881 ctgaccataa ccggtcagat cggaattgat ttgagtttcc cctttaaaac caagacgggc
     5941 ataggtcatg tcgccattgc caccgtaact gttttcaccg ttacccttgg aaaaataatg
     6001 cagaccaaca gctttaccgt acagatctac tttgttgcca tctttgttat agatttctgc
     6061 agcgtttgca gtacctgcta ctaacagagc agggacgatc actgccagaa tattgcgctt
     6121 catcattatt tattaccctc atggtttttt ttatgacacc tgccactgcc gtcaataagt
     6181 tctgtcaata aaaatttacg gaactattga tgagagtttg gtgtctttat gtgtctgcag
     6241 gcatctttcc attcaaacta acgtttcgct accgtgaaag tgctacaaag ataaagattt
     6301 agtttcaaaa agaaaaaata tgtaaccaaa agtaaaaatt aaggaacttt gtgaacaccg
     6361 tcatatttcc atagaaacgt gatgatattt acagcaattt ttatctattt atatgattta
     6421 cttatattta aattaactaa acggaaattt tgtttctgat ggaaacttta tcgacctggc
     6481 acaaaatcct ctttcagaca tccagaatgc caaaaaatag tatgaaaatt gtgctatcag
     6541 ctaagaaaaa actaatccgc aataagaatg cgggttattt tttgtgcgta aatgttgcaa
     6601 tgtaatattc cgtaacagga tgatcgttat ggcggggttg caaatagatt gcttgactta
     6661 acaaacagat aacttgacag aaaggataaa aaagagccag caggcgctgg ctctttgagg
     6721 aagaaaatca gaagctggcg ttgcgcggag tacgtgggaa cggaatcaca tcacgcacgt
     6781 tttgcacgcc agttacgtaa gcaatcaggc gttcaaaacc aagaccgaaa cctgaatgcg
     6841 gaacagtacc gtagcgacgc agatcgcgat accaccagta atcttcttta ttcaggccca
     6901 tttccagcat acgctcgtcc agaacgtcca gacgttcttc acgctgggag ccaccaatga
     6961 tctcaccgat gcccggagcc agaacgtcca tagccgcaac ggttttaccg tcttcgttga
     7021 ggcgcatata gaacgcttta atatctttcg gatagttttt aaccactacc ggtgctttaa
     7081 agtgttcttc cgccagataa cgctcatgct cagaagagag atcgacgccc cagtaaaccg
     7141 ggttttcaaa cttcctgccg cagttttcga gaatggtcac tgcgtcggtg taatccacct
     7201 gcgcgaaatc ggcctcaatg aagcgttcca gacgtgaaac ggcatcttta tctacgcgtt
     7261 cagcgaagaa tttcatgtcg tctgcgcgtt cttcgagaac cgctttgaag acatatttca
     7321 gcatggcttc agccagaccc gcgatatcgt tcaggttagc aaacgccact tccggctcca
     7381 gcatccagaa ttccgccagg tgacggctgg tgttggagtt ttcagcacgg aaagtcgggc
     7441 cgaaggtata aattttggat aatgcgcaag cgtaggtttc gccgttcaac tggccagata
     7501 cggtcaggaa agactcttta ccaaagaagt ctttgtcgaa atccactttg ccctgatcgt
     7561 tacgcggcag gttttccaga tccagcgtag aaacgcggaa catttcgcct gcaccttcgg
     7621 tatcagatgc ggtaatcagt ggcgttgaaa cccagaagaa tccctgctcg ttaaagaagc
     7681 gatgcagcgc ctgcgccagc gtatggcgaa cgcgcgcgac ggcaccaatc aggtttgtgc
     7741 gcggacgcag gtgagcgact tcacgcagat actcaatgct gtggcgtttt gccgccatcg
     7801 ggtaagtgtc tggatcttca acccaaccag caacttcaac cttgctggcc tgaatttcaa
     7861 attgttgccc ctggcccggc gatgccacga ctttacccgt cacaatgacc gagcagccgg
     7921 tggtcagacg caggacgtct tcattgtaat tgggcagaga attattgatg acagcctgta
     7981 caggatcaaa gcaggaaccg tcataaacgg cgaggaagga gatgccagct tttgaatctc
     8041 ggcgggtacg tacccatccg cgtacggtga cttcgctgtc aacggctacg cggccctgga
     8101 gtacgtcggc tacaggcaca acgctcataa tattctctct gttaatagtc ggaaaaaata
     8161 aacacttgtc cacccgtaaa tgggggggga tacctatgtt acctggcatc tgcaatcaga
     8221 caagcagaat tcgcaaatgc agcgaaagat ttcggaatta aaggtaaaaa aagggagccg
     8281 attagctccc tgatgatatt aactggcttt tttaatatgc ggaaggtcga acgctttgcg
     8341 cagcgcccga acaaacgctt tatcatggca gatagttttg ccagggctgt cagaaagttt
     8401 cgccaccggt ttaccgttac actctaccaa cttaatcaca atattcaggg gttttacctg
     8461 ggggatatcg caggtcaggc gagtcccaat accaaaactt aattgcacgc gggaagagaa
     8521 gtggcggtat agctcaaccg ctttgcgtaa atccagattg tcagagaaaa ccagcgtttt
     8581 actctgtgga tcaattccca gcttttcata atgtgcaatg gctttttcac cccattcaac
     8641 cgggtcgcca gagtcatgac gcaggccctg ataccgacta gcgaactcga caccgaaatc
     8701 acgcaggaaa gcatccatag tgatgcagtc ggttaatgca atgccaagtt ggtcgggata
     8761 ctcttccagc caggcagcaa gtgcagctcg ctggctgttg gctagatccg ggctgatttg
     8821 ctgatgtgcc tggaaccatt cgtgtgcctg tgttcccatc ggcgtgaggg aaagccgacg
     8881 cgccagatcg tagttgctgg tgcccacgaa ccaggattcc tgttgcagac gcttaacgat
     8941 ggtttcttgt acttcgcgag aaaaacggcg acgggtgcca aaatccatca gatggaagcg
     9001 cgacatatca agaccggcgg ttaacgccga gaagtcgact aatttgcttt ccagcgtgtc
     9061 gagggcttgc gcaacgtcgg cctgcggtga gcgatagcga tgtaccattt cactgataac
     9121 cgccagcaaa ggaacttccc agaggatgac ttcacgccac ggaccgctta agcgaatatc
     9181 cagcttgcca ttatcgttgg acacggtgac ttgttccggg ttaaagcgga actcgcgtaa
     9241 ccagttaaga tagtcggcct taaagaaagg cagggcagag agccactgat attcatcatc
     9301 ctgcaggcgc aggtgctgca tcgcctgaat ctgttcacga atagcatcgg cataaatacc
     9361 cagcagatcg tcacctcggc aacgaaactc cgccgcgaca tgcacatcgt aatagtgatg
     9421 aaacacggct tgctgcatat gcaacttata agcatctgta tccagcaacg agtgcagaac
     9481 aggagaagcg aattgtgtca taggtgcgca gtagcgtcct cgtacaggag cgtttagtac
     9541 aataaacatc ttcaggaact gctggagtat accttgtttc gcaatttatt gaaccccgat
     9601 cacaccatat gccacctttc tggtcgatgg cattacgcgg tgtatgttat aaaaatgtag
     9661 caataaaggc gtttgtacct gaaaagatga agattctgca tagcgcgatt tacgcaacag
     9721 gaatagactg aacaccagac tctataaaag atgctaaagg ttatttatga ctcaacagcc
     9781 acaagccaaa taccgtcacg attatcgtgc gccagattac cagattactg atattgactt
     9841 gacctttgac ctcgacgcgc aaaagacggt cgttaccgcg gtcagccagg ctgtccgtca
     9901 tggtgcatca gatgctccac ttcgtctcaa cggcgaagac ctcaaactgg tttctgttca
     9961 tattaatgat gagccgtgga ccgcctggaa agaagaagag ggcgcactgg tcatcagtaa
    10021 tttgccggag cattttacgc ttaagatcgt taatgaaata agcccggcgg cgaataccgc
    10081 gctggaaggg ctttatcagt caggcgatgc gctttgcacc cagtgtgaag ccgaaggttt
    10141 ccgccatatt acgtattatc tcgaccgccc ggacgtgctg gcgcgtttta ccaccaaaat
    10201 tattgccgat aaaaccaaat atcccttcct gctttccaac ggtaaccgcg ttgcgcaagg
    10261 tgaactggaa aacggacgcc attgggtaca gtggcaggac ccgttcccga aaccgtgcta
    10321 cctgtttgcg ctggtggcag gcgactttga tgtactgcgc gacaccttta ccacgcgttc
    10381 tggtcgcgaa gtggcgctgg agctgtacgt cgatcgcggc aaccttgatc gcgcgccgtg
    10441 ggcgatgacc tcgctgaaaa actcaatgaa atgggatgaa gaacgcttcg gcctggagta
    10501 tgacctcgac atctatatga tcgtcgcggt ggatttcttc aatatgggcg caatggagaa
    10561 taagggtctg aatatcttta actccaaata tgtgctggcc cgcaccgaca ccgccaccga
    10621 caaagattac ctcgatattg aacgcgttat cggccatgaa tatttccata actggaccgg
    10681 taaccgagtg acctgccgcg actggttcca gctcagcctg aaagaaggtt taaccgtctt
    10741 ccgcgatcag gaattcagct ctgaccttgg ttcccgcgca gttaaccgca tcaataatgt
    10801 gcgcaccatg cgcggattgc agtttgcgga agacgccagc ccgatggcgc acccaatccg
    10861 cccggacatg gtcattgaga tgaacaactt ctacaccctg accgtttacg agaagggcgc
    10921 ggaagtgatc cgtatgatcc acaccctgct gggcgaagaa aacttccaga aagggatgca
    10981 actctatttc gagcgtcatg atggtagcgc agcgacctgt gacgactttg tgcaggcgat
    11041 ggaagatgcg tcgaatgttg atctctccca tttccgccgt tggtacagcc agtccggtac
    11101 gccgattgtg accgtcaaag acgactacaa tccggaaact gagcagtaca ccctgaccat
    11161 cagccagcgc acgccagcca cgccggatca ggcagaaaaa cagccgctgc atattccgtt
    11221 tgccatcgaa ttgtatgaca acgaaggcaa agtgatcccg ttgcagaaag gcggtcatcc
    11281 ggtgaattcc gtgctgaacg tcacccaggc ggaacagacc tttgtttttg ataatgtcta
    11341 cttccagccg gtgcctgcgc tgctgtgcga attctctgcg ccagtgaaac tggaatataa
    11401 gtggagtgat cagcaactga ccttcctgat gcgtcatgcg cgtaatgatt tctcccgttg
    11461 ggatgcggcg caaagtttgc tggcaaccta catcaagctg aacgtcgccc gtcatcagca
    11521 agggcagccg ctgtctctgc cggtacatgt ggctgacgct ttccgcgcgg tgctgctcga
    11581 tgagaagatt gatccggcgc tggcggcaga aatcctgacg ctgccttctg tcaatgaaat
    11641 ggctgaactg ttcgatatca tcgacccgat tgctattgcc gaagtacgcg aagcactcac
    11701 tcgtactctg gcgactgaac tggcggatga gctgctggct atttacaacg cgaattacca
    11761 gagcgagtac cgtgttgagc atgaagatat tgcaaaacgc actctgcgaa atgcctgcct
    11821 gcgcttcctt gcttttggtg aaacgcatct ggctgacgtg ctggtgagca agcaatacca
    11881 tgaagcaaac aatatgaccg atgcgctggc agcgctttct gcggcggttg ctgcacagct
    11941 gccttgccgt gacgcgctga tgcaggagta cgacgataag tggcatcagg acggtctggt
    12001 gatggataaa tggtttatcc tgcaagccac cagcccggcg gcgaatgtgc tggagacggt
    12061 gcgtggtctg ttgcagcatc gctcatttac catgagcaac ccgaaccgta ttcgttcgtt
    12121 gattggtgcg tttgcgggca gcaacccggc agcgttccat gccgaagatg gcagcggtta
    12181 ccagttcctg gaggaaatgc ttaccgacct caacagccgt aacccgcagg tagcttcacg
    12241 tctgattgaa ccgctgattc gcctgaaacg ttatgatgcc aaacgtcagg agaaaatgcg
    12301 cgcggcgctg gaacagttga aagggctgga aaatctctct ggcgatctgt acgagaagat
    12361 caccaaagca ctggcttgat acttttgagc caaaatgcct gatgcgacgc ttgacgcgtc
    12421 ttatcaggcc tacaactccg ttgaaattgc tggttttgta ggctggataa ggcgttcacg
    12481 ccgcatccgg cagccgtgcc caactgcctg atgcgatgct tgacgtgtct tatcaggcct
    12541 acaagtccgt tgaaaatgct ggttttgtgc gccacatccg gcatattaac catgacgccg
    12601 aatcagttgt tcagaatgcc cacgccgcat tacccgttgc aatacttccg cttccagctc
    12661 cgctaatcgc accgacccta atcgacgcgg acgggggata tccactgtca aatccagacc
    12721 aatttttccc tcttcaatta acaacacccg gtcagccatt gctaccgctt cgctcacatc
    12781 atgcgtcacc aacagtacgg taaagccgtg ctgctgccaa agtgacacaa tcaaatcctg
    12841 catctcgagt cgcgttaagg cgtccagtgc cccgagcggt tcatcaagca acaataaacc
    12901 cggtcgatga atcaacgctc ttgccagcgc cacacgctgt ttctgcccgc cagaaagtgc
    12961 ggcaggccat tccccggcgc gattctccag tcctactgca gccaacgccc gacgcgcggc
    13021 atcgcgccac tggcctttaa ggcctaaccc aacgttatca atcaccgatt tccacggcag
    13081 cagacgcgca tcctgaaaca tcattcgcgt gtcttcctga atctcagcca gcggtgtggt
    13141 gcccgctaac acatcgcctg cggttggcgt ttccagacct gccagcaggc gcagcagggt
    13201 acttttgcca ccaccgctgc ggcccaccac cgccacaaac tgacctgccg gaatatgtaa
    13261 atccagttgg ttcaggacga tattttccgc gtaatgtttg cttactgcat tgagcaacaa
    13321 tggtgtgccc tggttcagac gagcagtatt cataccgtgg cctccttcaa atgataagcc
    13381 gggttccagc gcaaccagag gcgctctaac agctgcgcgc tgacgtcagc aagtttgccg
    13441 agcagggcgt aaagaataat agcgactacc accacgtccg tttgcaaaaa ctcccgcgca
    13501 ttcatcgcca gataaccaat gcctgaattg gcagaaatgg tttcggcaac aatcagcgtc
    13561 agccacatca ggcccaacgc aaaacgcacg ccgaccataa ttgagggcag ggcaccaggc
    13621 aggatcacat ggataaacag cggtatgccg gataatccat agctacgcgc catttccacc
    13681 agaccgcgat cgatattacg gatgccatgc caggtgttga tataaatggg gaatagcgtg
    13741 ccgagcgcca ccagaaagat ttttgcggat tcatcaatgc caaaccacaa aatcaccagc
    13801 gggatcagcg ccagatgcgg tacgttgcgc aacatctgaa ttgaggtatc cagcagccgt
    13861 tctccccagc gtgacagccc gctaatcagt cccagaatca agcccagcga tccgccaatt
    13921 gaaaagccaa tcagcgcccg ccaggaactg atcgccagat gctgccacaa ttcgccgctg
    13981 gcggagagcg tccagaacgc cgtcaccact ccttccggtg aaggcaaaat acgcgtcgac
    14041 agccagccaa ccgaggaggc cagttgccac accgccacga tgcccaccgg taaaaaccag
    14101 ggggcaacgc gcaataacca cttcttcact ggcgttgcca tcatcgtgtt ccttagcttt
    14161 gcgcgacttt acgggggata aaatcattcg ccaccgcttc gccttgcgga ttcagcggct
    14221 gcggctgggg aatttccgga atggcgacat ccagatgtgg gaacagcaac tcgccaaccc
    14281 gatacgcttc ttccagatgc gggtagcccg aaagcacaaa gctgtcgatg ccaagcgcgg
    14341 cgtattcgtt gattcgcgcg gcgaccgtag gaccatcgcc caccagcgcc gttccggcac
    14401 cgccgcgcac taagccaacg cccgcccata aattggggct gatctccaga ttgtcgcgct
    14461 tgccgttatg tagcgccgcc attcgctgtt gtcctacgga atccgtccgg gcgaatgcgg
    14521 cctgtgcttt ggcgatagtt tcatcatcaa gatgcgagat taaccgctcg gcggcctgcc
    14581 acgcttcgtc gttagtttca cgaacaatca catgcagacg aataccgaaa cgaattttgc
    14641 gtccatgcgc ggcagctttc gcccgcactt gttcgatctt ctcttttacc agctctggcg
    14701 gttcgcccca ggtgaggtag agatcaactt gttctgccgc cagctcctgg gcgacatctg
    14761 acgatccgcc aaagtaaagc ggaggatacg gctgttgaat cggcgggaag agcagttttg
    14821 ctccccgcac atgaatatgt ttaccgttga aatcgacgct ttcccccagc aataagcgcc
    14881 ccgccagacc tgggtaaatt ccgccgaggc ttcgtagcgc tcgctatgat caaggaacac
    14941 gccgtcgcct gccaactctt gtggatcgct gcctgtgacc agattaaaca acgcacgacc
    15001 atttgagaga cggtcaagcg tggcggcctg gcgagcggca acggtaggtg aggttacgct
    15061 gggacgcagg gcgacaagaa acttcagccg ctgcgtcacc gggatcatcg acgccgccac
    15121 cagccacgca tcttcacacg atcgccccgt tgggatcagc acaccggtat agcccagacg
    15181 atccgccgct tgcgcaattt gttgcagata gccgtgatca accgggcgtg aaccttcttc
    15241 cgttccaaga taatgcccgt caccatgggt cggtaaaaac cagaacatat tcagactcat
    15301 aattgttttc cttccaggtg agtgggctgc cagatgcgct ggcgaatatc aacttttttc
    15361 gacaccagac ggttttcata aaacagatct gccgtttgct gctgtaaggc ggcaacttca
    15421 gcgctcaccg gtttgatggt ggtgggagga cgatgatcca gatacgaggc aatcaccggt
    15481 gccggtaagc ccatcgtttt tgccagtaaa gcgatgcttt gctcgcgctg gctgcgggtt
    15541 aacgcatcgg cctcactaaa ggttgccagt acgccctgaa taaaagcgcc gtttttttct
    15601 gcataggggc gagctgccag ataaaacgat ccagtttgat tgagatcggt gccgtctttc
    15661 agcacccgca cgccgccctg taataacgca gcggagtagt agggatccca gatagcccag
    15721 gcgtcaacgt taccttgctg gaacgcggcg cgggcatcag ctggcgtcag ataagtgggc
    15781 tggatgtcag taaacttaag tccggcctga cgcagtgcac gcagtaaaag gttgtgtgaa
    15841 ctggaacctt tctgaaaggc aactttgtga cctttaagat cggctacggt tttgatcggg
    15901 ctgttttctg ccaccagaat cacttcggct ttgggcttcg gtggctcgac gcccacatat
    15961 atcaaatcag ccccggcagc ctgggcaaag attggcggaa tatccccggt actgccgaga
    16021 tcaatactgc caacgtttaa cgcttccaac atttgcggac ccgcggggaa ctccacccag
    16081 gagatttttg attgcggata gcgtttttcc agtaactggt ggctttttgc caataccata
    16141 ccaatactgc ctttctgata gcctatacgt aacgcttcag gcgaggattc tgcagcaacc
    16201 gcaaaagtag agacgctaag caatcccgcc agcgccagtt taatgatgtt acgcatggac
    16261 attacctcgc agagacagaa ggtcaggaac ctgaacatcg cggcggtgca atgcctgcca
    16321 gaaagtttct agcgcggtat caagacgggt ttgcagattt ggcgtgaact ggggtttgtg
    16381 atggtaatca ataacttgtg agtcatcggc aaacacgccg tgcaggagct cctgggcttt
    16441 catcgcactt aaaactggct taagggcata atcgaccgcc agcagatgag ccaccgtacc
    16501 gcccgtcgcc agcggtagca ccactttgcc ttgcaaagcg cgctctggca gcaggtcgag
    16561 cagggttttc agcgcgccgg aataggcggc tttatacaca ggtgtagcga ctatcagtcc
    16621 atcagcctgt tgcagctgtt cggtgaaggt cttgagtgcc ggactatcga aacgagcgta
    16681 aagcagatct tccggggcga agttttgcag attccagtga taaacctcta catccaggcc
    16741 atttagtttt tcccgcgcat attccagcaa ggagctggag cgagaaggaa agcgaggact
    16801 acccgccagg gtgatgacac gcatactctc tccttataac caattgttct ttttttgtta
    16861 acattgataa caattcggtc agtctgtcgg agagacaaga aaatgccaaa tataaatttt
    16921 gtgtatcttt ttctgaaaat aaactaaaga aagggcttta tatatcacgc atatttattt
    16981 gttggatagt cattagatgt tgaatcaatg aattactcgt tcagttaata tttaaatatt
    17041 catgaaatct ataaattaaa gatttgtcac ttattggatt tagtatgctt gatttaaatg
    17101 gcggtcaatt attgttatta ttaagtctaa tgtcatttaa ag
//
LOCUS       DAJTZL010000085        16865 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.085, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000085 DAJTZL010000000
VERSION     DAJTZL010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16865
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.085"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..728
                     /locus_tag="OO544_002407"
     CDS             <1..728
                     /locus_tag="OO544_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCR6767986.1"
                     /translation="ALILADLAMLEYAAERYPHIERHVSVQASATNEEAINFYHRHFD
                     VARVVLPRVLSIHQVKQLARVTPVPLEVFAFGSLCIMSEGRCYLSSYLTGESPNTVGA
                     CSPARFVRWQQTPQGLESRLNEVLIDRYQDGENAGYPTLCKGRYLVDGERYHALEEPT
                     SLNTLELLPELMAANIASVKIEGRQRSPAYVSQVAKVWRQAIDRCKADPQNFVPQSAW
                     METLGSMSEGTQTTLGAYHRKWQ"
     gene            737..1615
                     /locus_tag="OO544_002408"
     CDS             737..1615
                     /locus_tag="OO544_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCR6767987.1"
                     /translation="MKYSLGPVLWYWPKEALEEFYQQAATSSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELTSMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            1821..2828
                     /locus_tag="OO544_002409"
     CDS             1821..2828
                     /locus_tag="OO544_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase-like monooxygenase"
                     /protein_id="HCR6767988.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(2946..4190)
                     /gene="mtr"
                     /locus_tag="OO544_002410"
     CDS             complement(2946..4190)
                     /gene="mtr"
                     /locus_tag="OO544_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HCR6767989.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSALGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMITLIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(4344..6233)
                     /gene="deaD"
                     /locus_tag="OO544_002411"
     CDS             complement(4344..6233)
                     /gene="deaD"
                     /locus_tag="OO544_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HCR6767990.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(6226..6306)
                     /gene="yrbN"
                     /locus_tag="OO544_002412"
     CDS             complement(6226..6306)
                     /gene="yrbN"
                     /locus_tag="OO544_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HCR6767991.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(6413..7297)
                     /gene="nlpI"
                     /locus_tag="OO544_002413"
     CDS             complement(6413..7297)
                     /gene="nlpI"
                     /locus_tag="OO544_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HCR6767992.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(7406..9541)
                     /gene="pnp"
                     /locus_tag="OO544_002414"
     CDS             complement(7406..9541)
                     /gene="pnp"
                     /locus_tag="OO544_002414"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HCR6767993.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMTARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSRDGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(9788..10057)
                     /gene="rpsO"
                     /locus_tag="OO544_002415"
     CDS             complement(9788..10057)
                     /gene="rpsO"
                     /locus_tag="OO544_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HCR6767994.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(10206..11150)
                     /gene="truB"
                     /locus_tag="OO544_002416"
     CDS             complement(10206..11150)
                     /gene="truB"
                     /locus_tag="OO544_002416"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HCR6767995.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(11150..11551)
                     /gene="rbfA"
                     /locus_tag="OO544_002417"
     CDS             complement(11150..11551)
                     /gene="rbfA"
                     /locus_tag="OO544_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HCR6767996.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(11715..14387)
                     /gene="infB"
                     /locus_tag="OO544_002418"
     CDS             complement(11715..14387)
                     /gene="infB"
                     /locus_tag="OO544_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HCR6767997.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEAHRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(14412..15899)
                     /gene="nusA"
                     /locus_tag="OO544_002419"
     CDS             complement(14412..15899)
                     /gene="nusA"
                     /locus_tag="OO544_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HCR6767998.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(15927..16379)
                     /gene="rimP"
                     /locus_tag="OO544_002420"
     CDS             complement(15927..16379)
                     /gene="rimP"
                     /locus_tag="OO544_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HCR6767999.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(16586..16662)
                     /locus_tag="OO544_002421"
     tRNA            complement(16586..16662)
                     /locus_tag="OO544_002421"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16626..16628),aa:Met,seq:cat)
BASE COUNT         3872 a         4686 c         4228 g         4079 t
ORIGIN      
        1 acgcgctgat cctcgccgac ctcgccatgc tggagtacgc cgccgagcgt tacccgcata
       61 ttgagcgtca tgtgtcagtg caggcttcgg cgaccaatga agaggcgatt aacttttatc
      121 atcgccattt tgatgttgcc cgcgtggtgc tgccgcgcgt gttgtcgatt catcaggtga
      181 aacagctggc acgggtcaca cctgtcccgc tggaagtctt tgcttttggc agcctgtgca
      241 ttatgtcgga aggtcgttgc tatctgtcgt cgtatctgac gggtgagtcg cccaacaccg
      301 tgggcgcgtg ttctccggcc cgtttcgtgc gctggcaaca aacgccgcag gggctggaat
      361 cccgcctgaa cgaagtgctg atcgaccgtt atcaggatgg cgaaaacgca ggttatccga
      421 cgctgtgtaa agggcgttat ctggtggacg gcgagcgcta tcacgcgctg gaagaaccaa
      481 ccagtctcaa taccctggaa ctgctgccgg agttaatggc ggcgaatatt gcttcggtga
      541 aaattgaagg ccgccagcgt agcccggcgt atgtcagcca ggtggcgaaa gtctggcgtc
      601 aggctatcga ccgttgtaag gccgatccgc aaaacttcgt accgcaaagc gcgtggatgg
      661 agacgctcgg gtcgatgtcc gaaggcacgc agaccaccct tggcgcgtat caccgtaaat
      721 ggcagtgaga agagcaatga aatattcctt agggccagtg ctgtggtact ggccaaaaga
      781 ggcgctggaa gaattttatc agcaggccgc caccagcagc gccgacgtga tttatcttgg
      841 tgaagcggta tgcagcaagc gccgggcaac caaagttggc gactggctgg agatggcaaa
      901 atcgctcgcc ggaagcggga agcagatagt gctctccacg ctggcgctgg tgcaggcatc
      961 ctctgaactg ggcgaactga aacgctatgt tgagaacggt gagtttctga ttgaagccag
     1021 cgatctcggc gtggtgaata tgtgcgctga acgcaaactg ccgttcgtcg ccgggcacgc
     1081 gctgaactgc tacaacgcgg tgacgctgaa aatattgctc aaacagggca tgatgcgctg
     1141 gtgtatgccg gtggagcttt cccgcgactg gctggtgaat ctgcttaatc agtgcgatga
     1201 gttggggatt cgcaaccagt ttgaagtgga agtcctgagc tacggtcatc taccgctggc
     1261 ctactccgcc cgctgcttta ccgcgcgttc ggaagaccgc ccgaaagatg agtgtgaaac
     1321 ctgctgcatt aagtatccga acgggcgcaa cgtgctgtcg caggaaaacc aacaagtgtt
     1381 tgtactcaat ggcattcaga ccatgagcgg ctacgtttac aacctcggta acgagctgac
     1441 atccatgcag ggattggttg atgtggtccg cctgtcaccg cagggtactg acactttcgc
     1501 gatgctcgac gccttccgcg ctaatgaaaa tggcgctgcg ccactgccgt tgacggcaaa
     1561 cagcgactgt aacggctact ggcggcggct ggctgggctg gagctgcaag cctaagtaaa
     1621 tatgctcgtc atacttcaag ttgcatgtgc tgcatctgca ttcgttcacc ccagtcactt
     1681 acttatgtaa gctcctgggg attcactctc ttgtcgcctt cctgcaactc gaattattta
     1741 gagcacagct cactttgtta acaactttaa ctactcttta atgcagtatt aaagattaat
     1801 cggtaacaaa gtgagctgtt atgactgata aaaccattgc gttttcgcta ctcgatctgg
     1861 cccccattcc cgaaggttct tcagcgcgag aagcattctc ccactctctc gatctcgccc
     1921 gtctggctga aaagcgcggc tatcatcgct actggctggc agaacaccac aatatgactg
     1981 gcattgccag tgctgccacg tcggtattga ttggctatct ggcggcgaat accaccacgc
     2041 tgcatctggg gtctggcggc gtgatgttgc ctaaccactc accgttggtc attgccgaac
     2101 agttcggcac gctcaataca ctctatccgg ggcgaatcga tttggggctg ggtcgtgcgc
     2161 caggtagtga ccaacggaca atgatggcgc tgcgtcgcca tatgagcggc gatattgata
     2221 atttcccccg cgatgtcgcg gagctggtgg actggtttga cgcccgcgat cccaatccgc
     2281 atgtgcgccc ggtaccaggc tatggcgaga aaatccccgt gtggttgtta ggctccagcc
     2341 tttacagcgc gcaactggcg gcgcagcttg gtctgccgtt cgcgtttgcc tcacacttcg
     2401 cgccggatat gttgttccag gcgctgcatc tttatcgcag caacttcaaa ccgtcggcac
     2461 gactggaaaa accatacgcg atggtgtgca tcaatattat cgccgccgac agcaaccgcg
     2521 acgctgaatt tctatttacc tcaatgcagc aagcctttgt gaagctgcgc cgtggcgaaa
     2581 ccggacaact gccgccgccg attcaaaata tggatcagtt ctggtcaccg tctgagcagt
     2641 atggcgtgca gcaggcgctg agtatgtcgt tggtgggtga taaagcgaaa gtgcgtcacg
     2701 gcttgcagtc gatcctgcgc gaaaccgacg ccgatgaaat tatggttaac gggcagattt
     2761 tcgaccacca ggcgcgcctg cattcgtttg aactggcgat ggatgtgaag gaagagttgt
     2821 tgggatagtg tgtcttaacg cgggaagcct tatccgagct ggcaacgctg tcctacgtag
     2881 acctgataag cgaagcgcat caggcattgt gtaggcagca gaaatgtcgg ataaggcacc
     2941 gctgattact gatacaccgg cagcaaatta aagctcgata agatatggac cagtgcgttg
     3001 ccaacgccaa acaccagaat cagcgtaatc atcggcttgc cgccccagac acggaatttc
     3061 gggctgccaa agcgtttacg cgatgcgcgg gctaacagcg ccggaacaat tgccgcccag
     3121 atggtagctg ctaaaccagc ataaccaatg gcgtacagga atccgttcgg gaacagtaga
     3181 ccacccacaa caggcggggc aaaggtcagc aatgccgttt tcaagcgccc cagggctgag
     3241 tcatcgaaac caaacagatc tgccagatag tcaaacaaac ccagcgttac gccaaggaac
     3301 gaactcgcta ccgcaaagtt tgagaacacg accagcagaa ggtccaggct acggctgttc
     3361 agcacgccgc ttaatgcctg taccagcaca tcaatattac cgcccttctc tgcaataccg
     3421 ataaactccg gacgcgggat gttacccatc gtcgccagca accagatggt atacagcgcc
     3481 agtgccatca gcgtaccgta caccaggcat ttcacgatgg ttttcggatc tttgccgtaa
     3541 tacttcatca ggctcggcac gttaccgtga taaccaaacg atgccagaca gaacggcagg
     3601 gtcatcagca aatacggcgc ataagatgca ttgctttcgg cgacgttgaa caatgtcgca
     3661 ggctgcacat gccccagtag gctaccgaag gtgaggaaga aggtaatgac tttcgccccc
     3721 agtacaatcg ccgtcatgcg gctgacggcc ttagtactca accacaccac aaacgctacc
     3781 agcagtgcaa aaccaaaacc cgccgcccgt gccgggacgt ttagcgacat ctctgcgaag
     3841 gtgtgatgca gaattgaacc actggcagaa atataggcat aggtcaggat atagagcaca
     3901 aaggcaatgg aaataccgtt gaccacgttc cagcctttgc ccagcaaatc tttggtgatg
     3961 gtgtcaaaac tcgaaccgat tcggtaattc aggttagctt ccagaatcat caagccggaa
     4021 tgcagcatac agaaccaggt aaagatcagc gccgccattg accagaaaaa ccacgccccg
     4081 gacatgacca ctggcagaga aaacatccct gcgccaataa tggtgccgcc gataatcacc
     4141 acgccgccaa gcagcgacgg tgacgtttgg gtggtggtta gtgttgccat gagagcttct
     4201 ctccagtgaa aaatagtgcg actgcgttgt tatgcattgc actgtaccag tacacgagta
     4261 caaaagacag aaaaaaagcc ccgatggtaa aaatcggggc tgtatatatt attttacaga
     4321 ttgtgttcgc tgttcagcga tgattacgca tcaccaccga aacgacgacg accggtagag
     4381 tcatcacgac gcggagcgcg gccttcacga cgttcgccgc taaaacgacg accatcacca
     4441 cggccacctt cacggcgttc accgctgaag ttacgaccgc cttcacgacg ttcgccaccg
     4501 aaaccacgac caccgccacg acgctcaccg ccagtatgcg gctgtgcatc gcccagtaac
     4561 tgcatgttca tcggcttgtt gagaatgcga gtgcgcgtaa agtgttgcag cacttcaccc
     4621 ggcatacctt tcggcagttc gatggtggag tgagaagcaa acagcttgat gttaccaatg
     4681 taacggctac tgatgtcgcc ttcgttagca atcgcaccaa cgatatgacg aacttcaaca
     4741 ccatcatcgc ggcccacttc aatgcggtac agctgcatat cgccaacatc acgacgttca
     4801 cgacgcggac gatcttcacg gtcaccacgc gggccacggt cgttacgatc gcgcggacca
     4861 cggtcatcac ggtcacggaa ttcacgtttc ggacgcatcg gcgcatctgg cggtacgatc
     4921 agagtacgtt caccctgtgc cattttcagc agtgccgcag ccagagtttc gagatccagc
     4981 tcttcacctt ctgcagtcgg ctgaattttg ctcagcagtg cgcggtattg atccagatcg
     5041 ctgctttcca gctgctgctg tactttagcg gcgaattttt ccagacggcg tttgcccagc
     5101 agttctgcgt tcggcagttc tacttccgga atagtcagct tcatagtacg ttcaatgttg
     5161 cgcagcagac gacgctcgcg gttctcaacg aacagcagcg cgcggccagc acgacccgca
     5221 cgaccggtac gaccgatacg gtgaacgtaa gactcagaat ccatcgggat atcgtagtta
     5281 actaccaggc tgatacgctc aacgtccagg ccacgggctg caacgtcggt cgcaatcagg
     5341 atgtccagac gaccatcttt caggcgttcc agtgtctgtt cacgcagcgc ctggttcatg
     5401 tcaccgttca gcgcggcgct gttgtagccg ttacgctcaa gagcttcagc cacttccaga
     5461 gtcgcgtttt tggtacgaac gaagataatc gccgcatcaa aatcttccgc ttccaggaaa
     5521 cgtaccagtg cttcgttttt gcgcataccc cagacagtcc agtagctctg gctgatgtca
     5581 ggacgggtag tcacgctgga ctgaatgcgc acttcctgcg gctctttcat aaagcggcgg
     5641 gtaatgcgac gaatcgcttc cggcatggtt gcagagaaca gagcggtctg atgaccttcc
     5701 gggatctgcg ccataatggt ttcaacgtct tcgatgaagc ccatgcgcag catttcgtca
     5761 gcttcatcca gaaccagacc gctcagttta gagaggtcca gagtgccacg tttcaggtgg
     5821 tccagcagac ggcccggagt accgacaacg atctgcggcc cctgacgcag ggcgcgtaat
     5881 tgcacgtcat aacgctggcc gccgtacaga gcaaccacat ttacgccgcg catgtgttta
     5941 gagaaatccg tcattgcttc agcaacctgt accgccagtt cgcgggtcgg tgccagcacc
     6001 agaatctgtg gtgctttcag ctcaggatca agattctgca acagaggtaa agagaatgct
     6061 gcagtttttc cgctccccgt ctgggccata cccagaacgt cgcggccatt cagcagatgt
     6121 ggaatacact ctgcctgaat tggagatggt ttttcgtaac ccagatcgtt aagggcttca
     6181 aggataggag ccttcaggcc cagatctgca aaagtggttt cgaattcagc catgtagtac
     6241 gtgtgcctca aaattaatgg cggccagtct acataactca tcatgaaatt gatcagcaat
     6301 tttcattgaa aagtgtgaac cggctcaaag taggtgtatt aacgaacaac aacgccctca
     6361 cccgttaagg tgatggcaat caaaaaagat tacgggctga tgtgtacgtc agctattgct
     6421 ggtccgattc tgccaggtca tcttggtcct ggcccaggag cgataattcc aacaatgcgt
     6481 atcggtgctc aacaaagtta tgaacgttgt tggcaaccgc cagtttgaac agtgccgtgg
     6541 cgctgtccaa atcccccaga cttaggtagt acttacctaa atagaagttg gtttcactga
     6601 gatgctcagc gagcgaggtg ttatccgttg cgtccgcctt gagcctttcc attaacgttt
     6661 gttcgctaat gttgcccagg tagaactcga caatgttcca tccccactgt tccttatccg
     6721 atttttcgaa gtgctgtttc aacacttctt tagcctgctt ctcatcgagc ttctgctcgg
     6781 cgagataaag ccacagacta cggaaaggat cattgggatc gtcttgataa aacgccagca
     6841 gatcatcttg cgctaacttg tcacgaccgc cgtaatataa tgcgatcccg cgattcaagt
     6901 gcgcgtagtt gtaagttgga tcaagctcaa gtacagaatc aaacgcttca taggcagcat
     6961 caaaattgcc tgcctgcgtt aaatatatgc ctaagtaatt gaatacttca ggcatatccg
     7021 gtcggattgc cagcgcttgc gaaaaatcgt tacgcgctaa tgccctcaga ccgagactat
     7081 catacaacac tccgcgctca tataaaagct gtgcgcgttc gtcatcggtt aaagcccgac
     7141 tggcaaggat ttgttccata cgtgccagaa tcacttcctg ctgtaaagtc ggttgcaatg
     7201 gtaccgcgag gacttcactt ttacgccagg aagtattact gcatcctgca agcgtaagtg
     7261 ctgtcgcaac gaaacaccag cgcaaaaaag gcttcatttc ccactcccga agaccacggt
     7321 tgaatgaacg tcctgttccc ggttgctaac aaggcgtcct gcccggttaa aagccccccg
     7381 ccgcagcgga gggcaaatgg caaccttact cgccctgttc agcagccgga gcttccggtg
     7441 ctgcagcagg ttgagactgc tcagtcgctt ctttaatgct cagacggata cggccctggc
     7501 gatcaacttc tagaactttc accggtactt cctgacccat ctgcaggtaa tcggtcactt
     7561 tctcaacgcg tttgtcagcg atttgagaga tgtggaccag accttcttta ccgccgccga
     7621 tggcaacaaa tgcgccaaag tcaacgatac gggtcacttt accagtgtag acgcggccca
     7681 cttcgatttc tgcagtgatc tcttcgatac gacgaatagc atgtttcgct ttctcgccgt
     7741 cggtcgctgc gatcttcaca gtaccgtcat cttcgatttc gatggtagtg ccagtttctt
     7801 cggtcagggc acggataaca gagccgcctt taccgataac gtctttgatc ttatccgggt
     7861 tgatcttgat ggtatggata cgcggtgcga actcagagat atcgccacgc ggcgcgttga
     7921 tcgcctgttc cattacgccc aggatatgca gacgcgcacc tttagcctgg ttcagcgcaa
     7981 cctgcatgat ctctttggtg ataccttcaa ttttgatatc catctgcagt gcagagatac
     8041 cgtcgcggga acctgcaact ttgaagtcca tatcgcccag gtgatcttcg tcgcccaaaa
     8101 tgtcagacag tacaacgtag ttgtcgcctt ctttcaccag acccattgcg atacccgcaa
     8161 cggcagcttt gattggcaca cctgcgtcca tcagcgccag agacgcgccg cacacggaag
     8221 ccatagaaga agaaccgttg gattcggtga tttcagacac tacacgtacg gtgtacggga
     8281 atttgtccat atccggcatg actgccagca cgccgcgctt cgccagacga ccgtgaccaa
     8341 tttcacgacg cttcggagaa ccgaccatgc cggtttcgcc tacggagtac ggagggaagt
     8401 tgtagtggaa caggaaggta tcggtacgtt cgcccatcag ttcatcaaga acctgcgcgt
     8461 cacgagcggt acccagcgtt gcagtaacca gtgcctgcgt ttcaccgcgg gtgaacagcg
     8521 cagaaccgtg agtacgcggc agcacgccag tacgcacatc cagaccacgg atcatatctt
     8581 tttcacgacc gtcgatacgc ggttcgcctg ccagtacgcg gctacgaaca acgtttttct
     8641 cgatagcgtg cagaatttca cccagttcgt tttcgtccag ggtttcgtct tcagcaagca
     8701 gcgtcgcgat ggtttcagat ttgatgacat caacctgcgc ataacgctct tgtttgtcgg
     8761 tgatgcggta agcatcactc agacgagctt cagccagtgc agcaacgcgc gcgttcagcg
     8821 cttcgtttac cggctccggc tgccagtccc aacgcggttt accggcttct ttcactagtt
     8881 cattgatgtt ctgaataaca acctgctgtt gttcatgacc gaacactact gcgcccagca
     8941 tctgatcttc gctcagcagt tcagcttcag attcaaccat cagtacagcg gcttcagtac
     9001 cggcaacaac cagatccagt ttgctctctt tcagctcgtc ctgagtcggg ttcagtacgt
     9061 actggtcatt gatgtaacct acgcgggcag caccaatcgg gccattgaac ggaataccag
     9121 acagagacag tgctgcggaa gcaccaatca tcgcgacgat atccgggtta acctgcgggt
     9181 taacagaaac cacggtggcg ataacctgaa cttcgttgac gaagccttcc gggaacagcg
     9241 ggcgaatcgg gcggtcaatc agacgcgcga tcagggtttc gccttcgctt ggacgacctt
     9301 cacgacggaa gaagctaccc gggatacgac cagcagcgta ggtacgctcc tgatagttaa
     9361 cggtcagtgg gaagaagtcc tgacctggtt tggctttttt ctgtccaaca acggtaacga
     9421 ataccgcggt gtcatccatg ctaaccataa cagcggcagt agcctgacga gccgtcatgc
     9481 cggtttccag agtcacggtg tgttggccgt actggaattt acgaacgatc ggattaagca
     9541 atgtaatatc ctttctcttt cttagacagt accttacggc actggtgtta atacccgatc
     9601 ttctgcgcat cctcgcgact aatgacaacc ctaacccagc tctatgtggg taaagcctct
     9661 cattagccgc gcgaacctct gcaacggaag atcattcata gcaacaatac attagtttcc
     9721 agtgaattgc tgccgtcagc ttgaaaaaag gggccactca ggcccccttt tctgaaactc
     9781 gcaagaatta gcgacgcaga cccaggcgct cgatgagctg ggtgtaacgt gctacgtctt
     9841 tacgtttcag gtagtcgagc agtttacgac gctgagaaac catgcgcagc agaccacgac
     9901 ggctgtggtg atcttttttg tgctctgcaa agtggccctg caggtggttg atctgtgcag
     9961 tcagcagtgc tacctgaact tcggtagaac cggtgtcgtt tgcgtcacga ccaaactcag
    10021 aaacgatttt agctgttgct tcagtactta gagacatttt aaaactccaa agtatataga
    10081 atgaaaggac gccgatctct aattcagcga tcccagtgta cgttacgcaa agtgttaaac
    10141 aatttacgcg acgttaagcg gcagtattct actcgtagcg acctgttatc gcaagacggt
    10201 taacattacg ccgggtattc aaccaccagg cgacgaggcg cgacgcggcc ttcatcgtca
    10261 atttcgccca taccgataaa cttgccgttc tcaccttctg taacgcgaac cagtccttcc
    10321 agcggcgcac cggatgtacg aactgggtta ccatttttga agtaaacaga agacgttaac
    10381 ggaagattca ccaccgggta gtccgaagct ggactgtcca ttggcatcag taatggatca
    10441 agtaactccg cagccggaat atcctgctgt tcggcttgtt cgaccagttc acgcagatgt
    10501 tccagggtca ccatccgttc aaccggatat ttacttaccg ccagacgacg caggtaaata
    10561 acatgcgcgc cacaaccgag tttttcaccc aggtcatcaa tgatggtgcg gatataagtg
    10621 ccttttgagc agtgaatttc cagctccagc tcattgcctt cgtggcgaat aaacagcaat
    10681 tcataaacgg taatcgggcg cgcttcacgc ggtacttcaa tgccctgacg cgcatattcg
    10741 tacagttttt taccctgata tttcagtgct gaatacatcg aagggatctg ttcgatatcg
    10801 ccacggaaag tatccagcgc cgccgccagt tgctctgcgc taaaggttac cggacgttct
    10861 tcaacgattt gtccgtcggc atcagaagta tcggtacgct gtccaagacg cgcaatgacc
    10921 cgatagcgtt tgtcggagtc cagcagatac tgggaaaact tcgtcgcttc cccgaggcaa
    10981 attggcaaca tgcctgttgc cagcgggtcc agcgcaccgg tatgcccggc acggttggcg
    11041 ttataaatcc gtttcacttt ttgcagcgca tcattgctgg acataccctg cggtttatcc
    11101 agcaacaaaa cgccgttaat gtcgcgaccg cgacgacgag gacgactcat tagtcctcct
    11161 tgctgtcgtc cgggttaaca cgacgttctt cgtcatgttt gaccacgctg gtcaccaggt
    11221 ttgacatgcg catcccttca accagagagt tgtcgtagaa gaaggtcagt tccggcacga
    11281 tacgcaggcg catcgctttc cccagcaggc tgcggatgaa accagaagct tcttgcaacg
    11341 ctttgatgcc cgctttaacc gcgtcttcat ctttgtcgtt gaggaacgtc acatatactt
    11401 tggcatacgc caggtcgcga gacatttcga caccggaaac ggtggtcatc atgcccaggc
    11461 gaggatcttt aatttcacgc tgcaggatga gagcgatctc tttttgcatt tcctgcgcta
    11521 cgcgctgcgg gcgaccaaat tctttcgcca taataaattc tcctgacaaa aaaggggctg
    11581 ttagcccctt tttaaaatta atttcaggtg gaagggctgt tcacgttggc ctgataagac
    11641 gcgccagcgt cacatcaggc aatccatgcc ggatgcggcg taaacgcctt atcccgcatg
    11701 gaaccctaaa aaccttaagc gatggtacgt tggatctcga tgatttcgaa tacttcgatc
    11761 acatcgccag tgcggacgtc gttgtagttc ttaacgccga taccacattc cataccgtta
    11821 cggacttcgt taacgtcatc tttgaagcgg cgcagggact ccagctcgcc ttcgtagata
    11881 accacgttgt cacgcagaac gcggatcggg ttgtgacgtt taaccacacc ttcggtaacc
    11941 atacagcctg cgatagcacc aaatttcggc gatttgaaca cgtcacgaac ttccgccaga
    12001 ccgataatct gctgtttcag ttccggagac agcataccgc tcatcgccgc tttcacttcg
    12061 tcaatcaggt tatagatgac ggagtagtaa cgcagatcca ggctttccgc ttcaatcact
    12121 ttacgtgcag aggcatcagc acgtacgttg aagccaacca ggatggcgtt ggacgccgca
    12181 gccagggtgg cgtcggtttc ggtgatacca cctacgccag aaccgatgat cttcacttta
    12241 acttcgtcag tagacagttt cagcaaggag tcggagatcg cttccacaga accctgtacg
    12301 tctgccttca ggacgatgtt cacttcgtga acttcgcctt cggtcatgtt ggcgaacatg
    12361 ttctcgagtt tagatttctg ctgacgcgcc agtttaactt cgcggaattt accctgacga
    12421 tacagtgcaa cttcacgcgc tttcttctcg tcacgtacaa cggtaacttc atcacccgca
    12481 gccggtacgc cggacaggcc gaggatttcc accggaatgg acggacccgc ttccagcact
    12541 tcctgaccca gttcgttacg catcgcacga acacgaccgt attcgaagcc acacagaacg
    12601 atatcgccct tgtgcagagt accttcacgt accagaacgg tagcaaccgg accacgacct
    12661 ttatcgagga aggattcgat aaccgcaccg ctcgccatac ctttacgtac cgctttcagc
    12721 tccagaactt ccgcctgcag caggatagcg tccagcagtt catcgatgcc ggtacccgct
    12781 ttcgcagata cgtgtacgaa ctggctttca ccgccccact cttccggcag gatgccatac
    12841 tgggagagtt cgttcttaac acgatccgga tcggcttctg gtttatcaat cttgttcact
    12901 gcaaccacca ccggtacctg cgccgctttc gcgtgctgga ttgcttcgat ggtctgcggc
    12961 atcacgccgt cgtcggcagc aacaaccagg actacgatgt ccgttgcctg cgcaccacgt
    13021 gcacgcattg aagtaaacgc ggcgtgcccc ggggtgtcca ggaaggtgat catgccgttt
    13081 tcagtttcaa cgtggtatgc accaatgtgc tgggtaatgc cgcccgcttc gccagaggca
    13141 actttcgttg aacgaatgta gtccagcaga gaggttttac cgtggtcaac gtgacccatg
    13201 atggtcacaa ccggcgcgcg cggttcagcc gcagcacccg tgtcacggtc gctcattacc
    13261 gcctcttcca gctcgttttc acgacgcagg ataactttgt ggcccatctc ttcagcaacc
    13321 agctgtgcgg tttcctgatc gataacctgg ttgatggttg ccattgcgcc cagtttcatc
    13381 atcgctttga tgacctgaga gcctttaacc gccatcttgt tcgccagttc gccaacggtg
    13441 atggtttcgc cgatcacaac gtcacggtta acggcctgag ctggcttctg gaagccttgc
    13501 tgcagggaag aacctttacg ttttccgcct ttaccgccac gtactgctgc gcgtgcttct
    13561 tcacgatcag cttttgattc agcgtgtttg ttgcctttct tcggacgcgc tgctttcgcg
    13621 ttacgaccac ggccacgacc gccttcgact tcacgatcgc tttcgtcttc tgcctggcga
    13681 gcatgttgag aagtagtgac gtgataatcg ctggaatctt cagtcggttc cgcgttatca
    13741 gtccatttgt tttcttccgc catacgacgt gcttcttcag caacgcgacg tgcttcttct
    13801 tcgagtttac gatgcgcttc ttcttcggct ttacgcttga gctctgcagc ttcctgctca
    13861 cggcgggctt tttcagcctg ggcgttttta gtcatatcgt cttgttgatt gctcactttg
    13921 tctttttccg cagcttcacg tttcgcttgt tcagcagctt cacgcttagc ttgttctgca
    13981 gcctcacgtt cagctttttg ttgcgcctcg cgtttagccg attcttctgc ctcacgacgg
    14041 gcttgctctt ccgcttcacg ctgcgcttgc tcttccgctg caaggcgttc agcctcttgc
    14101 ggatcgcgtt tcacaaaggt gcgtttcttg cggacttcga tttgtaccga tttgcttttt
    14161 ccaccggtac caggaatgtt aagggtgctg cgtgttttac gttgcagcgt caatttgtcc
    14221 gggcctgaat ttttctgatt caggtggtca atcaaagtct gtttctcttg tgcagacaca
    14281 gagtcgtcag cagacttccg gatacctgca tcagcaaatt gctgtaccag gcgttccacg
    14341 gaggtctgtc gctctgcggc cagcgtttta atcgttacat ctgtcatgct gttccttcct
    14401 gctacagttt attacgcttc gtcaccgaac cagcaaatat tacgggcagc cataatcagt
    14461 gctccggcct tttcgtcggt caacccttcg atatcagcca gatcatcaat gccctgttcg
    14521 gcgagatctt ccagcgtaca aacgccacgg gcggccagtt tgaatgccaa atcacgatct
    14581 accccttcaa ggttcagcag atcgtcagcc ggtttgttat caccgaggct ttcttcctgg
    14641 gcctgtgcaa tggtggccag tgcattttta gcacgctcgc gcagtgcttc aacggtcggc
    14701 tcatcaaggc cttcgatttc caacagctct ttcatcggca cataggccag ttcttccagc
    14761 gtcgagaagc cttcttctac cagaacagtc gcgaagtctt cgtcgatgtc gagatatttg
    14821 gtgaaggtgt cgatcgctgc gtgcgcttcc gcctgatgct tagcctgcag gtcgtcaacg
    14881 gtcatcacgt tgagttccca accgctcagc tgcgaagcca gacgcacgtt ctgaccgtta
    14941 cggccaatcg cctgcgccag gttaccggct tcaacggcga tatccatggt gtgtttatct
    15001 tcatccacca cgatagaagc aacgtctgcc ggtgccattg cgttaatcac gaactgcgcc
    15061 gggttatcat cccacaggac gatatcgata cgctcgccac ccagttcagt agacaccgcc
    15121 tgaacacgcg cgccacgcat acctacgcaa gcacctaccg gatcgatacg tttatcgttg
    15181 gttttcaccg cgattttcgc acgagaaccc ggatcgcgag ccgctgcttt aatttcaatc
    15241 acttcttcgc cgatttctgg cacttcaata cggaacagtt cgatcagcat ttccggcttg
    15301 gaacgagtga cgaacagttg cgcgccacgc gcttccgggc gaacggaata aagcacgcca
    15361 cgaacgcggt cgccagggcg gaagttttca cgcggcagca tatcttcacg caggatcacg
    15421 gcttcagcgt tgttgcccag atccagagag atgttgtcgc ggtttacttt tttcaccacg
    15481 ccggtgatga tttcaccttc gtgttcacgg aactgatcaa ccaccatcgc acgttcggct
    15541 tcacgcactt tctgcacgat aacctgtttt gccgtctggg tagtgatacg gtcaaaggta
    15601 acagactcaa tctgatcttc aacgtaatcg cccaggttca ggctttcatc ttcataacgt
    15661 gcggcttcaa gggtgatttc cttggtcggc tgggtgactt catcaacaac taaccagcga
    15721 cggaaggtgt caaaatcacc gcttttgcga tcgatctgta cgcggacgtc gatctcttgt
    15781 tcatattttt tctttgttgc tgtcgccagc gcgctttcca atgcttcgaa aatcttctcg
    15841 cgaggtagcg ccttttcatt ggatacggct tcaactacag ccaaaatttc tttgttcatc
    15901 gcgggctttt cacctcatcc agactattaa aagtggggaa ccaggttcgc cttctggata
    15961 ttactcagcg cgaacacttc atctttacct tcgacggtaa ctgtgatcat ttcaccgtct
    16021 accgctttga taacgccctg ccatttacga cggttttgta ccgccatacg gagaaccaga
    16081 gtcacctctt ctccgacaaa acgggcgtag tgttcagccg tgaacagtgg gcgatcgaga
    16141 cccggtgagg agacttccag gttataagca acggtgatgg gatcttcaac atccagcaca
    16201 gcacttacct ggtggctcac atcagcacaa tcatcaacat tgatgccatc ttcactatca
    16261 atatagatgc gcagtgtgga tgtgcgaccg cgaataaatt cgatgccaac cagttcaaaa
    16321 cccagggcct caactggcgc agtaatcatc tctgttaatt tttgctctaa tgtggacaag
    16381 cccaccccca agacataaaa aaagggccta aagcccagtt attctgtagt cagataacaa
    16441 aaaaccccga taaatcgggg ctttatataa ctgaacccta taaccgcaac tgcggtctgg
    16501 agcactttcc agaaggattt tttcaaatcc cactacgaag gccgaagtct tcacagtata
    16561 tttgaaaaag gactctaagg gaaagtggtt gcgggggccg gatttgaacc gacgaccttc
    16621 gggttatgag cccgacgagc taccaggctg ctccaccccg cgcctgaaac gtggcaaatt
    16681 ctactcgttt tgggtaaaaa atgcaaatac tgctgggatt tggtgtaccg agacgggacg
    16741 taaaatctgc aggcattata gtgatccacg ccacattttg tcaacgttta ttgctaatca
    16801 tgtgaatgaa tatccagttc actttcattt gttgaatact tttgccttct cctgctcccc
    16861 cttaa
//
LOCUS       DAJTZL010000086        16742 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.086, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000086 DAJTZL010000000
VERSION     DAJTZL010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16742
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.086"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..787)
                     /gene="wzxE"
                     /locus_tag="OO544_002422"
     CDS             complement(<1..787)
                     /gene="wzxE"
                     /locus_tag="OO544_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HCR6768000.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISD"
     gene            complement(789..1919)
                     /gene="rffA"
                     /locus_tag="OO544_002423"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(789..1919)
                     /gene="rffA"
                     /locus_tag="OO544_002423"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HCR6768001.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGERFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(1924..2598)
                     /gene="rffC"
                     /locus_tag="OO544_002424"
                     /gene_synonym="wecD"
     CDS             complement(1924..2598)
                     /gene="rffC"
                     /locus_tag="OO544_002424"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HCR6768002.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAQLLTPDVLAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGGIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(2576..3457)
                     /gene="rfbA"
                     /locus_tag="OO544_002425"
     CDS             complement(2576..3457)
                     /gene="rfbA"
                     /locus_tag="OO544_002425"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCR6768003.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     DIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(3476..4543)
                     /gene="rffG"
                     /locus_tag="OO544_002426"
     CDS             complement(3476..4543)
                     /gene="rffG"
                     /locus_tag="OO544_002426"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCR6768004.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPHGVVHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(4543..5805)
                     /gene="wecC"
                     /locus_tag="OO544_002427"
     CDS             complement(4543..5805)
                     /gene="wecC"
                     /locus_tag="OO544_002427"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HCR6768005.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSTRASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(5802..6932)
                     /gene="wecB"
                     /locus_tag="OO544_002428"
     CDS             complement(5802..6932)
                     /gene="wecB"
                     /locus_tag="OO544_002428"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HCR6768006.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQTMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(6988..8034)
                     /gene="wzzE"
                     /locus_tag="OO544_002429"
     CDS             complement(6988..8034)
                     /gene="wzzE"
                     /locus_tag="OO544_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HCR6768007.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(8046..9149)
                     /gene="wecA"
                     /locus_tag="OO544_002430"
     CDS             complement(8046..9149)
                     /gene="wecA"
                     /locus_tag="OO544_002430"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HCR6768008.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(9389..10648)
                     /gene="rho"
                     /locus_tag="OO544_002431"
     CDS             complement(9389..10648)
                     /gene="rho"
                     /locus_tag="OO544_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HCR6768009.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(10733..10834)
                     /gene="rhoL"
                     /locus_tag="OO544_002432"
     CDS             complement(10733..10834)
                     /gene="rhoL"
                     /locus_tag="OO544_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HCR6768010.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(10975..11304)
                     /gene="trxA"
                     /locus_tag="OO544_002433"
     CDS             complement(10975..11304)
                     /gene="trxA"
                     /locus_tag="OO544_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HCR6768011.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            11435..12700
                     /gene="rhlB"
                     /locus_tag="OO544_002434"
     CDS             11435..12700
                     /gene="rhlB"
                     /locus_tag="OO544_002434"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HCR6768012.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            12834..14318
                     /gene="gppA"
                     /locus_tag="OO544_002435"
     CDS             12834..14318
                     /gene="gppA"
                     /locus_tag="OO544_002435"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HCR6768013.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIANAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVEKEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQESQWQSYVHWPLEVH"
     gene            complement(14365..16386)
                     /gene="rep"
                     /locus_tag="OO544_002436"
     CDS             complement(14365..16386)
                     /gene="rep"
                     /locus_tag="OO544_002436"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HCR6768014.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
BASE COUNT         4061 a         4563 c         4193 g         3925 t
ORIGIN      
        1 catcggaaat actgctcacc ccttgccaga tccccacttc atcccagcta tactgcgccg
       61 ccagcagttt acgcatcatg atgtaagcaa caggcaaggt caccgacgta atcaacgcca
      121 tgagcgtaaa tttgctcaac tgccctgcca gaccgttatc ccagctgggt ttcagatagc
      181 ttaacgggat gacaccacgt ttgatcaaca tgatggcggc aggaatcacc accagcgcgg
      241 gaatcagcgc cagacccagc aacgcccctt cataaccgcc caaacggtaa ctgacgtagt
      301 acgcgagaac gccaatcaag ctgccgacaa tcagcgataa cgcattacct gcggcatcgc
      361 gaaagccttt catcagcgcc agtaacaggt tgccccaggc gatccccatt tgcaccagcg
      421 ccactaaacg caccagcccc tgatagtcgg tattaccaaa cagtccctgg ctgattggcg
      481 cagctgccag cacaaaaacc agcgccatca gcgtagagaa accaagtacc atcgctgatg
      541 aagtgccgac cacgcggcgc agctgttgcg gattatcatg gtactgggca acgtatttgg
      601 ttacaccgtt aaagatgcca gctccggcaa gcacgccgag cacggtaatc aactggcgga
      661 aatttgccgc cagcccaagc cccgccggac caaatgacac agccagcaac ttaccgacca
      721 gtaacccggc accaatcttg accagtgtac tggccgccgt ccacaaggac gcttttgcca
      781 acgacatatc aggagaagta gttcaacaaa gtcgcaatta ccgtacgctg attgacgggc
      841 gacaggttgt agaacagcgg caggcgcagc aggcgctcgc tctctttggt ggtgtagcga
      901 tcttcaccgt ggaactcacc aaagcgttcc cccgcagggc aaccgtgcag cggaatgtaa
      961 tggaacaccg ccatgatttc cgcttctttc agaaagttaa tcaacgcgct ccggtcatca
     1021 atatcccgca gtttaatgta gaacatatgc gcgttctgca cgcagccatc gggaatcgac
     1081 ggcagctcga tacgcccggc tttcgccaga ggcgctaacg catcgtagta gttttgccac
     1141 agcgccagac gttgctggtt gatacgatcc gctgcttcca gttgcgccca caggtacgca
     1201 gcttgcagat cggacatcaa atagctggag ccaatatcgc gccaggtata tttatcgacc
     1261 tgaccacgga agaactggct gcggttagtg cccttttcac ggatgatctc ggctcgttcg
     1321 attaacgctt tatcgttaat cagcgtcgcg ccgccttcac cacccgccgt atagtttttg
     1381 gtttcatgga agctaaagca gccaatatga ccaatggttc ccagtgcacg ccctttgtaa
     1441 gtggacatca cgccctgagc ggcatcttct accacaaaca gattatgctt tttcgccaac
     1501 gccataatgg tgtccatttc gcaggccaca cccgcgtaat ggaccggcac gataacgcgc
     1561 gttttgtcgg tgatcgccgc ttcaatcagc gtttcgtcga tgttcatggt gtccgggcga
     1621 acatccacaa aaacgatttt tgcgccacgc agcacaaagg cattggcggt ggagacaaag
     1681 gtgtagctcg gcatgatcac ttcatcgcca ggctggatat cgagcagcag cgccgccatc
     1741 tccagcgaag cggtgcagga cggcgtcagt aacactttgg cgctgccaaa acgttgctcc
     1801 agccactgct ggcagcgacg ggtaaaaccg ccatcgccac acagtttgcc gctacccatt
     1861 gccgactgca tatagtcgag ttcggttccc accaccggcg gtgcgttaaa tggaatcatg
     1921 tgatcacctg tataaccagt acgcggtgct ttctacattc gcaccacttt gtatgtatcg
     1981 tttaagcgcg gcggtgttgc ccatttgggt cgccacccgc aaagttgttt taccgcgagc
     2041 atacgcccag tttagcgccg tttgcatcag ctcagcacct gcaccgcgtc cagccagcag
     2101 gccaattcgc gcatctgtcg cattgagttc ccgtaaagag acataaccgc gaataccgcc
     2161 ggacgccgca cgtaaaatca gacattgatg atcaaaggtg ccgcgcacgg cattttcaat
     2221 ccactgtgca taaaagcgtc cgctggcgtc aggcgcatac cacggcgcac gaaaacggct
     2281 ttgcgcaaat gcggcgctgg ctaactgacg taatgcggga atatcggtct cttgtgccac
     2341 tacagcacca ctatcactga cattgttcac gggtagtgcc aaatcaactt caccttctac
     2401 cagggagaat cccagctgtt gcagggcatc cagttcaccc gtatttgatg ccgcaatttt
     2461 ggcctgcacc cgtgaccacg gcgctaacac gtctggcgtc aggagctgtg cttcagacgt
     2521 aatgcgcacg atggcgctgt taacaccaaa gaaggcgttt tcccaggtta gtggctcaat
     2581 actggcgcgg acgggcacga agtaactcca gcagatattg gccgtagcca gttttcgcta
     2641 atgaactggc agcacgcttc acaccctcgt catcgagcca gccgttacgc caggcgatct
     2701 cttccaggca ggcaatctta aagccctggc gtttttctac tgtctgcaca aaggtactgg
     2761 cttcaatcag gctgtcgtga gtgccagtgt ccagccaggc aaatccgcgc ccgagcagtt
     2821 caacggtcag gttgcccgcc tcgaggtaca tctggttgat ggaggtaatc tccagttcac
     2881 cacgctccga cggcttcacc tgctttgcgt actccacgac tttactgtcg tagaaataaa
     2941 gcccggtcac cgcccagttt gacttcggct gttttggctt ttcttccagc gagatagcgc
     3001 ggaaattgtc gtcaaactcc accacgccaa agcgttccgg gtccatcacc tgatagccaa
     3061 ataccgtcgc cccttccgtg cgcgccgcaa catgacgcag cttcggactg aagccctgac
     3121 cgaagaagat gttatcgccc agcaccagac aagaaggttc accgttgagg aaggtttcac
     3181 cgatgataaa ggcctgcgcc agaccgtccg ggctgggctg ttcggcgtat tccagctgga
     3241 taccgaactc actaccatcg cccagcaggc gctggaaata acctttatct tccggcgtag
     3301 tgatgatgag aatttcgcgg atatcggcca acatcagcac cgacagcgga tagtaaatca
     3361 ttggcttatc gtaaatcggc aacagttgct tcgatacgcc gcgcgtaatc ggatgcaatc
     3421 gggtgccgga accgcccgcc aggataatac ctttcatgtt tgcctccgct gaaaattagc
     3481 ctttcagacc taaacgctcg ccctgatagc tgccgtcctg cacctgcttc caccagcttt
     3541 cattagccag ataccactgc accgttttac gcattccact ttcaaaggtt tcctgtggca
     3601 gccagccaag ttcacgggca attttcgaag catcaatggc ataacgcaga tcatgccccg
     3661 gacggtcagc gacaaaggtg atcaagtcac gatagtgcac cacgccgtgc ggcttgttcg
     3721 gagccagttc ttccagcagc tcgcaaatgg tttccacaac atcgagattc ttacgctcgt
     3781 tgtgaccacc aatattatag gtttcaccga ctttcccggt ggtcgccacg caatacagcg
     3841 cgcgggcgtg atcttccaca tacagccagt cacggatttg ctgcccgttg ccatataccg
     3901 gcagcggttt acccgccagc gcgttgagga tcatcagcgg gatcagtttt tccggaaagt
     3961 ggtaaggacc gtagttattc gagcagttgg tgataagcgt cggcagaccg taggtccgca
     4021 gccaggcgcg caccagatgg tcgctgctgg cttttgacgc ggaataaggg ctgctcggcg
     4081 catacggcgt ggtttcggtg aagaaatcat ccgtcgagtg caggtcacca tatacttcgt
     4141 cggtggagat atgatgaaaa cggaacgctg attttttatc ttccgtcagc gtattccagt
     4201 aagcccgcgc cgcttcaagc aatgtataag tcccgacaat gttggtttca ataaacgctg
     4261 ccgggccgtc aatagaacgg tcaacatggc tttcggctgc cagatgcatg acacagtctg
     4321 gctgatgctc agtgaatacg cgtgccagtt ctgcccgatc gcagatatca actttctcaa
     4381 aggcaaagcg ctcgctttgc gcgaccggtg ccagcgacat caggtttccg gcgtaggtca
     4441 gcttatcgac cactaccacc gcgtcgctcg tttcgttgat gatataacgc accagcgccg
     4501 agccaataaa cccggcacca cctgttatca gaatttttct catcagcgcc agactccttt
     4561 ggcatcgacg acatactgct gatggacatt gtcgccattg ataactttaa actgactatg
     4621 atcgaccagc atcaccagca cgtctgccgt tgccagcgcc tcgtcaagct gcgccagagt
     4681 acaaagcccg gtcagttttt tcggcaactg gtggatgtta ggctcaacaa ccagagtttc
     4741 gccgctatgc cactgggcga tcagttcagc gatttccatc gccgggcttt cgcgcaggtc
     4801 atcaatattc ggtttaaacg ccagaccaaa gcaggcgatt ttcagttcac tggcgcgttt
     4861 atcggtagcc gccaggcaat cagccaccgc cgctttcacc tgatcgataa cccagaacgg
     4921 tttgtgatcg ttcacttcgc gcgcggtacg gataagccgc gcctgctggg ggttctgtgc
     4981 cacgataaac cacggatcaa cagcaatgca gtgaccgccc acgccagggc caggctgaag
     5041 aatattaacg cgagggtgac gattcgccag gcgaatcagt tcccagacgt taatcccctg
     5101 atcggcacaa atcagcgaca attcattagc aaaagcgata ttcacatcgc ggaagctgtt
     5161 ttcggtgagc ttacacattt ccgccgtccg cgagttagtg acgacacact caccttcgag
     5221 gaaaattttg tacagttcgc tggcgcgggt cgaacaaacc ggcgtcatac caccaatcac
     5281 gcgatcgttt ttaatcagct cgaccataac ctggcccggt aacacgcgtt ccgggcagta
     5341 agcaatgttg acgtccgcct gctcgcccac ctgctgtggg aaagtgagat ccggacgcat
     5401 ctctgctaac cattctgcca tcttctcggt tgaccccacc ggcgaggtgg attcaaggat
     5461 caccagcgcg ccttttttca gcactggcgc aatggagcga gcagccgatt caacgtaggt
     5521 catatctggc tcatgatcgc ctttaaacgg cgtgggtaca gcaatcagcc aggcatccgc
     5581 ttcaactggc gtcgtgctcg ctcgtaaaaa accgccttct acggcagttt ttactacgct
     5641 cgccaaatca ggctcgacga tatggatttc gccacgattg atggtatcaa ccgcgtgttg
     5701 gttgatatcg acaccaatca cctgtttttg ccgtgaggca aacgctgctg ctgtaggcag
     5761 cccgatgtaa cccagtccga taacagaaat ggtcgcaaaa ctcatagtga tatccgatta
     5821 ttttttaacg cttccagaat gcgagagcat gcctgaccat caccatacgg gttatgggcg
     5881 cggctcatag tttgatattc gttttcgtct tttaaaagac gcgtcacttc ctcgacaatt
     5941 cgctgcttat ccgtgcctac cagacgcacc gtacccgccg tcaccgcttc cggacgctca
     6001 gtggtatcgc gcatcaccag caccggtttc cccagcgaag gcgcttcttc ctgaatgccg
     6061 cctgagtcgg tcaaaatcag ccaggcgtgg ttcatcagcc agacaaacgg taaatactcc
     6121 tggggatcga tcagaatgac atttttcaca tgccccagaa tgcgattgac cggctctctg
     6181 acgttcgggt tgagatgcac cggatagaca atctggatgt cctggtgcgt ggtggcaatg
     6241 tctgccaacg cgtggcagat ttcttcaaag ccacgaccga aactttcacg cctgtgaccg
     6301 gtcaccagaa tcatcttttt atcggggtcg ataaacgggt aatttgccgc cagttctgaa
     6361 cgcagcgtat cgctgctcat cacctggtca cgcacccata acagtgcatc aataactgta
     6421 ttaccagtaa tgaagattcg gctatccgca acgttttcac gcagcaagtt ttgccgggaa
     6481 gtttcggttg gagagaagtg atacatcgcc agatgcccgg tcaatgtacg gttagcctct
     6541 tccggccacg gcgaataaag atcgcccgtg cgcagaccag cctcaacgtg accaacagga
     6601 atacgctgat aaaacgccgc caggctggtt gccagcgtcg tcgttgtgtc gccgtgaacc
     6661 agcacgacgt ctggtttgaa ctcggcaaga ataggtttta gcccttccag aatccgacag
     6721 gttatctctg tcaggccctg tcctggctgc attatgttga gatcgtagtc aggtacaatg
     6781 gaaaagagtt tcagcacctg atcgagcatc tcccgatgct gcgcagtgac gcaaacttta
     6841 gcctcaaaaa aaggatcttt tgccaacgca tgcaccaacg gcgccatctt gatggcttcc
     6901 gggcgcgtac caaatacagt cagtactttc acatcgattc tcttcgaata agcggcgagc
     6961 gcctttgcgc tcaccgcagc agtgttgcta tttcgagcaa cggcgggtta atgcgacacc
     7021 agccccgatc agccccccga caatgcccca cataatcatc aggaaggcac gacgtgggct
     7081 atcgcgtttt accggttctt ccggcgtacg caaatagcga taggtctgaa aacgcggatc
     7141 cagggttgga ccaacattca gggtgtttaa catggcccga ttctgatcat agtcgagatc
     7201 aaaggccgga ccgacggcct gtaaattttc cagtcgagcc tggagcattg gacgcccaag
     7261 caggaacatt tctgaatcag gtaattcctc ggcaggcaca tctgtcgcac tgcgcgaaat
     7321 attatgctgc tcagcaattt tcagcgcctg ttcaatactg ttcatccggc ggtcgtagat
     7381 ggctttcgcc acctcttcct gacgcttcac ctgtgctttc atctggatgg tacgcgccgc
     7441 ccatgcgcct ttcagctcat cattcagatg gctggctgca cgctggctgg caaaagcaac
     7501 atactgacgt aacaggttat tagcgtcagg cgcggtttca gcaataagct tcacgctgtc
     7561 attgaccgcg cgggtaaagt ctccggggat aaactggatg ttgttaatca tttcatccag
     7621 caacgccgca tcggctttgc tgttgcccac catccgctgt ttgtaatagt cggtttgcag
     7681 ccagaactct ctgcgggtat cccacgaggc cagttgcata acaaactctt tataggcttc
     7741 gtccatgacc gatggttggt cggcagaagc catgtttgaa cggacatcca ggttacgcaa
     7801 aaattgctgc tgcgagtaat atccccccag catattcacc gttggacgat cggtaatcgc
     7861 cgtcgagctc cactcctgac gagcaaaaaa agtatacgcc agcgcgatta acgcaaatgc
     7921 cagccccatg ccaataatcc atagcttccc agcccacaag gtacgaaaca acccacgaat
     7981 atccagttca ttttcagcgt cttcggccgg tttcccaggc attggttgtg tcatcacatc
     8041 ctcatttatt tggttaaatt ggggctgcca ccacgatttc tacgcagtct gcgttttacg
     8101 cgcttaataa agcgagcaac tttccaggca cgcttaatgc aatatccata gaggaagaat
     8161 gctagcaaaa agagcaccag catgacccac tccgggacaa aatgagaata ttctgccagc
     8221 acgccaatgg aagcgagcag tgccgcagca agggtaatca gcacaaacgc ctgacgggaa
     8281 gtaaacccgg cacgcatgat caaatggtga atatgctgac ggtcaggaga gaatgggctc
     8341 atgcctttac gcaggcgacg gtacataatc gccaccatat ccattagcgg aatggcgatt
     8401 atccacaaag cggtaaccgg gctgatggga tgggttttgc cctgggtcgt ttcgagcagg
     8461 atccagataa cggtaaaacc aatcagcgta ctgcccgcat cacccataaa gactttgtag
     8521 cggcgaccca ggataccaag gttaagcatg atgtatggca ggatggcggc gatcatcgca
     8581 aagcaccaga ttgcgaggct ggtttgcccg tcgaaccaca aaatcatacc gattgctgca
     8641 aacgagacgc aggacaaccc gcccagcaag ccatcaatgc catcaaccat gttgaacgca
     8701 ttaatggccg cccagacggc aaatagcgtc aggaagtaac caaacggtcc gagcaccatc
     8761 tcccaggagc caaagatata acccaggcta ctgagataaa gtttgccgaa caccatcata
     8821 acaatgccaa cagcggcctg tatggtggca cggattttta cgctgatatc aaaacggtca
     8881 tccagcgcgc caatgaaaac aagcacaccg gcacaagcga gatagagaga tgcatgcgga
     8941 atatagtaat cgacaattcc gaacgtgaag caaatccctg cgtaaaccga aatcccccca
     9001 acgagaggta tcaatccctg gtgacgtttg cggaagtttg gtttatccac taaaccgact
     9061 ttttttgcca ccttacgggc aaaaaacaga aacagtgtcg tgaataaaaa aatactgatg
     9121 agatcagtac tcactgtcag taaattcaca atgcatgctc tcagagaaaa ttattagcag
     9181 aagtataacc acgaagacct ttattcagaa gggaaagtcc gttccgaacc caccagtcct
     9241 gtgcaaaaat taaccatttg atatgcaaat tgcataacac gccagcaaat tccaggaatt
     9301 ttcctaacat cgccagcgcg gcattaagat tacagatata aaagcaaaac gccacgtaaa
     9361 cacgtggcgt ttttggcata agacaaattt atgagcgttt catcatttcg aagaaatcgt
     9421 cattggtctt ggtcatggcc agtttattaa tgaggaattc cattgcatcg atttcgccca
     9481 tcgggtgaat gattttgcgc aggatccaca ttttctgcag ttcttcctga gtagtgagca
     9541 gttcttcttt acgggtaccg gaacggttgt agtcgatagc cgggaagacg cgtttttcag
     9601 cgatcttacg agagaggtgc agttccatgt tgcctgtacc tttaaactct tcgtagataa
     9661 cttcgtccat tttagaaccg gtatcgataa gcgccgtcgc gataatagtc aggctgccgc
     9721 cctcttccac gttacgcgcc gcaccaaaga agcgtttcgg acgatgcagg gcgttggcat
     9781 ccacaccacc ggtcaacact ttacctgacg ccggaacaac ggtgttgtaa gcgcgcgcca
     9841 gacgagtgat ggagtcgagc agaatgataa cgtctttctt gtgctcaacc aggcgtttgg
     9901 ccttctcgat caccatttcc gcaacctgaa cgtggcgaga tgcgggttcg tcaaaggtag
     9961 aagcaacaac ttcacctttt accagacgct gcatctcggt tacttcttcc ggacgttcgt
    10021 cgatcagcag aaccatcagc acacaatccg ggtggttgta agcaatgctc tgggcaatgt
    10081 tctgcagcag catggtttta ccggctttcg gcggtgccac aatcagacca cgctgaccac
    10141 gaccgatagg tgatgccaga tccagtacgc gagcagttaa atcttcagta gaaccgttac
    10201 cacgttccat acgcagacga gaatttgcgt gcagcggggt taagttctca aagaggattt
    10261 tgttgcgggc gttttcaggt ttgtcgaagt taacttcgtt aactttcagc agcgcaaaat
    10321 agcgttcacc ttctttcggc gggcgaatct taccagagat ggtatcacca gtgcggaggt
    10381 tgaaacggcg gatttggcta ggggaaacgt agatgtcatc aggaccggcg aggtaggagc
    10441 tgtctgcgga acggaggaaa ccaaatccat cctgcaatat ctccagtacg ccatcaccaa
    10501 agatatcttc gccactcttt gcgtgctgct tcaggatggc aaaaataatg tcctgcttac
    10561 gcatgcgagc caggttttcc agccccatat tttcgccgag agtgatcagc tcagaaaccg
    10621 gcgtattctt taattcggta agattcatag tggtgtgagt tcttaaactt ggggtaaatc
    10681 tcgaacttaa tgttgtgaat ggtatggcag gatcatccat gcctgtttac ggtcatcgac
    10741 tcatgtcttt tcgctgcctg gtcacaggag agtacgcaga actgaaacga caagacggat
    10801 tgagtgacga gccagaaatc tgttcacttc gcatttaaga taaaacggga agcgttggaa
    10861 attacaagat tcaaacttaa taaggtatgt ttaatacgaa gtcaacacta agttagcatg
    10921 actcacgccg ggcgtccagt ttttagcgac ggggcacccg aacatgaaat tcccttacgc
    10981 caggttagcg tcgaggaact ctttcaactg acctttagac agtgcgccca ctttggttgc
    11041 cgccacttca ccgtttttga acagcagcag agtcgggata ccacggatgc catatttcgg
    11101 cgcagtgcca gggttttgat cgatgttcag ttttgcaacg gtcagtttgc cctgatattc
    11161 gtcagcgatt tcatccagaa tcggggcgat cattttgcac ggaccgcacc actctgccca
    11221 gaaatcgacg aggatcgccc cgtccgcttt gagtacatcc gtgtcaaaac tgtcgtcagt
    11281 caggtgaata attttatcgc tcatatataa ctccacagga ataagcctgg cgtgttggtt
    11341 tcgttgttgg tgtaacatta accaactaaa ggttgacttt atttcaccgg atacgctttc
    11401 gtaaagcaat agtaagctga tattctacca cactatgagc aaaacacatt taacagaaca
    11461 gaagttttcc gacttcgccc tgcatccgaa ggttgtagaa gcccttgaaa aaaaagggtt
    11521 tcataactgt acgcccattc aggcactggc ccttccgctg acgctggcgg gtcgtgacgt
    11581 agccgggcag gcgcaaaccg gtaccgggaa aacgatggcg tttcttacgt caacgtttca
    11641 ttatcttctc tctcatcctg cgattgccga tcgcaaggtg aatcaaccgc gtgccttaat
    11701 tatggcaccg acgcgtgaac ttgccgtgca gatccatgcc gacgcagaac cgctggcgga
    11761 agctactggc ctgaagctgg gtctggctta cggtggtgat ggctacgaca aacagctgaa
    11821 agtgctggaa agcggcgttg acattctgat tggcaccacg ggtcgtttaa ttgactacgc
    11881 caagcagaac cacattaacc tcggtgccat tcaggtagtg gttctggatg aagccgatcg
    11941 catgtacgat ctgggcttta ttaaagatat ccgctggctg ttccgccgta tgccgcctgc
    12001 aaaccagcgc ctcaacatgc tgttctccgc cacgctttcg taccgggtac gtgaactggc
    12061 gttcgagcag atgaacaatg ccgaatatat tgaagtggaa ccggaacaga aaacgggcca
    12121 ccgtattaaa gaagagcttt tctacccttc taacgaagaa aaaatgcgtt tgctgcaaac
    12181 gctgatcgaa gaagagtggc cagacagagc gattattttc gccaatacca aacaccgttg
    12241 tgaagagatc tggggccacc tggcggcaga tggtcatcgt gtcggtttgt tgacaggcga
    12301 tgtcgcgcag aaaaaacgtc tgcgtattct tgatgaattt acccgtggcg atctggatat
    12361 tctggttgcc accgacgttg ccgcgcgtgg tttgcatatt ccggcagtga cgcacgtctt
    12421 taactacgat ttacccgatg actgtgaaga ttacgttcac cgtattggtc gtacaggtcg
    12481 cgcaggcgca agcggtcact ctatcagcct ggcgtgtgaa gagtatgcat tgaatttgcc
    12541 tgctattgaa acctatattg gtcactcaat tccggtaagc aaatacaatc cggacgcatt
    12601 gatgaccgat ctgccaaaac cgctgcgcct cacgcgccca cgtacaggca atggtccgcg
    12661 tcgtactggc gctccgcgta atcgtcgtcg ttcaggttaa ataaaatgtc atactcaaaa
    12721 tcattcaaac tgtatcaaga tggcaagaga atgaatcccg atgagcatac tgtagtaagt
    12781 gattcaggtg aaagaatgca gccaacacag agacagattg aaggatgaag agtatgggtt
    12841 ccacctcgtc gctgtatgca gccattgatc tcggttcgaa tagttttcat atgctggttg
    12901 tgcgcgaggt ggctggaagc atccagacgc tgacgcgaat aaaacgcaaa gtgcgtctgg
    12961 ctgctggcct gaatagcgaa aatgccctgt ctaatgaagc aatggagcgc ggttggcaat
    13021 gtctgcgcct gtttgctgaa cgtctgcaag atatccctcc ctcgcaaatt cgcgttgtcg
    13081 ctacggcgac attacgtctt gccgtcaatg cgggtgattt tattgccaat gcgcaggaaa
    13141 tcctcggttg tccggtacag gtgatcagcg gtgaagagga agcacgtctg atttatcagg
    13201 gcgttgctca cacaactggc ggtgccgatc agcgcctggt ggtggatata ggcggtgcca
    13261 gtactgaact ggtaaccggc acgggtgcac aaaccacctc gttgttcagc ctgtcgatgg
    13321 gctgcgtcac ctggctggaa cgctattttg ccgatcgtaa tctggggcag gaaaattttg
    13381 atgctgcaga aaaagcggca cgcgaagtgt tacgtccggt tgccgatgaa ttacggtatc
    13441 acggctggaa agtgtgcgtt ggtgcttccg gcaccgtgca ggcgttacag gaaatcatga
    13501 tggcacaggg gatggatgaa cgcattaccc tggaaaagtt gcagcaattg aaacagcgag
    13561 ccattcattg cggtcggctg gaggaactgg agattgacgg gctgacgctg gaacgtgcgt
    13621 tagtgttccc gagtggtctg gcgatcctga tcgccatttt taccgaactg aatattcagt
    13681 gtatgaccct ggcgggcggt gcgctgcgtg aaggcctggt ctacggcatg ttgcatctgg
    13741 ccgtcgagca ggatattcgc agccgtacgc tgcgtaatat tcagcgccgc tttatgatcg
    13801 atattgatca ggcgcagcgc gtagccaaag ttgcggctaa cttcttcgat caggtggaaa
    13861 aggaatggca tcttgaagca ataagccgcg atttgctcat cagcgcctgt cagcttcatg
    13921 aaatcggcct gagcgttgac tttaaacaag cgccgcaaca cgctgcttat ctggtgcgta
    13981 atctggatct tcccggtttt acccccgcac agaaaaaatt gctggcgacg ctactgctca
    14041 accagactaa tccggtcgat ctctcatcgc tgcatcagca aaatgccgta ccgccgcgcg
    14101 tcgcagaaca actctgccgt ttactccgcc tggccatcat ttttgccagc cgtcgccgtg
    14161 acgatctcgt gccagagatg acattacagg ctaaccatga actgttgacc ttgacgcttc
    14221 cgcaaggttg gctaacccaa catccgctgg gtaaagagat tattgctcag gaaagccagt
    14281 ggcagagcta tgtccactgg ccgctggaag tgcattaatg agtaagtgcc ggatgcgatg
    14341 ctgacgcatc ttttccggcc ttgattattt ccctcgtttt gccgccatca tcgctttcag
    14401 attcgccaga tggctttgcc ctttctgcat ccgttcttcg gcgctgacca ctttgcgctc
    14461 ctgttcccaa atcagatcat cctgcggcag ctccagcaaa aagcggctcg gctccgggcg
    14521 caccagctcg ccgtactgac ggcgttcttt acacagcgta aaggtcaatt ccttctgggc
    14581 gcgggtaatg ccgacatagg ccagccgccg ctcctcatcg atattatctt catcgatgct
    14641 gctctggtgc ggcaaaaacc cttcttccat accgaccatg tagacataag gaaactccag
    14701 cccttttgac gcgtgcagag tcatcagttg cacctgatcc agctcttctt cactctcacc
    14761 acgctccatc atgtcgcgca aagtaaagcg cgtcaccacc tgggtgagcg tcatcggctc
    14821 atccagttcg ctgccttcca gcatctccgt catccagcta aacagttggt tgacgttctt
    14881 catgcgcatt tcggcggctt tcgggctggg cgatgtttcg tacagccagg attcataatc
    14941 catgccgtgg atcagatcgc gcaccgcggc aatcggctcc cgctccgcca gacgctggat
    15001 ttctgccaac cagtgagtaa agcgggtcaa tgcttcataa ccacgtccgc taagcgtctg
    15061 actcaggccc atatcaaagc tggcggtaaa catgctttta ttgcgcgtca tcgcccactc
    15121 acccagcttt ttcagcgtcg ccgggccaat ctctctcttc ggcgtgttaa cgatacgcag
    15181 aaatgcgctg tcatcgtccg ggttagtcag cacgcgcaga taagccagca agtccttgat
    15241 ttcaggacga gagaaaaacg acgtaccacc agaaattttg tacgggatgc ggttttgcat
    15301 caggaatttt tcaaacactc gcgactgatg gttaccgcga taaagaatag cgtaatcttt
    15361 gtactgcgtt ttattgacga agtgatgcgc aatcagttca ccagtgacgc gctcggcttc
    15421 atgttcttcg ttattcgcgc ttaaaacttt aagctctgtg ccgtaaccca gttcggagaa
    15481 cagacgcttc tcaaagacgt gcgggttatt ggcgatcagg atgttcgccg ctttcaaaat
    15541 acgtccggaa gagcggtagt tctgctcaag cttaatcacc ttcagcgccg gaaaatcctg
    15601 actcagcagc accaggtttt gtggacgtgc gccgcgccag gagtagatcg actggtcgtc
    15661 atcgcccacc acggtaaagc gcgcgcggct gcccaccagc agtttcacca gttcatactg
    15721 gctggtgttg gtgtcctgat actcatccac cagcagatag cgaattttgt tctgccagcg
    15781 ctcgcggact tcttcattgc gttgcagcag caacgtcggc aataaaatca gatcatcgaa
    15841 gtcgagaaca ttgcacgctt tcaggtgtgc atcatacagc ccataacaat gggcaaaaat
    15901 acggtcccgc tcgccaatcg cactggctgc cgcctgggac ggtgttttga gatcgttctt
    15961 ccagttagag atggtcgaaa tcaactgttg caggagaact ttgtcatctt caatcagccc
    16021 ttcggtcaac tctttgagca acgccagctg atcggtatcg tcaaacagcg aaaagttcgc
    16081 cttcatcccc agcgccgcat actcacgctt gatgatatcc agccccagcg tgtggaaggt
    16141 tgagatcatc agcccacgcg cctctttgcg acccagcgtc tgtccgacac gctctttcat
    16201 ctcgcgcgcc gccttattgg taaaggtcac cgccgcgata tgtcgcgcct gataaccgca
    16261 accgcggatc agatgggcga ttttattggt gatcacacga gttttaccgg aacccgcgcc
    16321 cgccagcacc aggcaggggc cggtaacgaa ttcgacagct tgttgttggc cggggtttag
    16381 acgcataggt gtattgctca atcttcgaac ggggggagga ttgtagcaga atcgcatttg
    16441 aatgtgaccg cgtgtaagcc gctaaccgat tggctgcgga tgtttaaatc agagagggaa
    16501 gaccgaaacc ggagttcatc gtgaatccat tagacttaga aaatatcggg tcgtaacgtt
    16561 gtgttattac tttttcagag tttactaagg agaggtgtac tcgtggcgat taatttcagt
    16621 cctaaggttg gtgaaatact ggattgcaac tttggtaact atcctgttag ccagaatgga
    16681 cagttcagca ccacgtatta cgatggccgc attcctcctg aaatgataaa aaacaggtta
    16741 gt
//
LOCUS       DAJTZL010000087        16295 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.087, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000087 DAJTZL010000000
VERSION     DAJTZL010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16295
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.087"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..577
                     /locus_tag="OO544_002437"
     CDS             <1..577
                     /locus_tag="OO544_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HCR6768015.1"
                     /translation="GRPNNPFEPRRNRYAQKYLTLALMGGAAFFVLKGCGDSSDVDND
                     GDGTFYATVQDCIDDGNNADICARGWNNAKTAFYADVPKNMTQQNCQSKYENSYYDNV
                     EQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASRPVWRNTSGDYSWRSGSGKKES
                     YSSGGFTTKKASTVSRGGYGRSSSARGHWGG"
     gene            580..1743
                     /locus_tag="OO544_002438"
     CDS             580..1743
                     /locus_tag="OO544_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HCR6768016.1"
                     /translation="MLRHNVPVRRDLDQIAADNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWHASDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRTTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLCKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIDSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            1827..3452
                     /locus_tag="OO544_002439"
     CDS             1827..3452
                     /locus_tag="OO544_002439"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HCR6768017.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQVLCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTTGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCL"
     gene            complement(3569..3844)
                     /gene="yjfN"
                     /locus_tag="OO544_002440"
     CDS             complement(3569..3844)
                     /gene="yjfN"
                     /locus_tag="OO544_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease activator YjfN"
                     /protein_id="HCR6768018.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(3993..4322)
                     /gene="bsmA"
                     /locus_tag="OO544_002441"
     CDS             complement(3993..4322)
                     /gene="bsmA"
                     /locus_tag="OO544_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HCR6768019.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            4504..5253
                     /gene="yjfP"
                     /locus_tag="OO544_002442"
     CDS             4504..5253
                     /gene="yjfP"
                     /locus_tag="OO544_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR6768020.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNNIIAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            complement(5250..6005)
                     /gene="ulaR"
                     /locus_tag="OO544_002443"
     CDS             complement(5250..6005)
                     /gene="ulaR"
                     /locus_tag="OO544_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HCR6768021.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ERGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(6113..7177)
                     /gene="ulaG"
                     /locus_tag="OO544_002444"
     CDS             complement(6113..7177)
                     /gene="ulaG"
                     /locus_tag="OO544_002444"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HCR6768022.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            7532..8930
                     /gene="ulaA"
                     /locus_tag="OO544_002445"
                     /pseudo
     CDS             7532..8930
                     /gene="ulaA"
                     /locus_tag="OO544_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
     gene            8946..9251
                     /gene="ulaB"
                     /locus_tag="OO544_002446"
     CDS             8946..9251
                     /gene="ulaB"
                     /locus_tag="OO544_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HCR6768023.1"
                     /translation="MTVRILAVCGNGQGSSMIMRMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            9261..9725
                     /gene="ulaC"
                     /locus_tag="OO544_002447"
     CDS             9261..9725
                     /gene="ulaC"
                     /locus_tag="OO544_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HCR6768024.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            9739..10389
                     /gene="ulaD"
                     /locus_tag="OO544_002448"
     CDS             9739..10389
                     /gene="ulaD"
                     /locus_tag="OO544_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HCR6768025.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            10399..11253
                     /gene="ulaE"
                     /locus_tag="OO544_002449"
     CDS             10399..11253
                     /gene="ulaE"
                     /locus_tag="OO544_002449"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HCR6768026.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLRLAKTLGFDFVEMSVDETD
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVVKARDWVKARMA
                     KAGTVEAA"
     gene            11253..11939
                     /gene="ulaF"
                     /locus_tag="OO544_002450"
     CDS             11253..11939
                     /gene="ulaF"
                     /locus_tag="OO544_002450"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HCR6768027.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATYLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGDAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSFLMNKHFMRK
                     HGPNAYYGQK"
     gene            12036..12587
                     /locus_tag="OO544_002451"
     CDS             12036..12587
                     /locus_tag="OO544_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1440 domain-containing protein"
                     /protein_id="HCR6768028.1"
                     /translation="MAINGLFVRTDPQRRRYGVALLVGVLSGIISAFVKWGAEVPLPP
                     RTFSGGRDEFNPPYIFLRDYLGIDPTQTVYTFSEHIINPVMVTHIIFSLVFAIGYCIV
                     AEIFPKVKLWQGILAGLIVTVAVHGIFCPALNLTPPLTQLPFDEYASEILGHIFWFWI
                     IEIMRRDLRNRITHEPDPEVAIR"
     gene            complement(12662..12977)
                     /locus_tag="OO544_002452"
                     /pseudo
     CDS             complement(12662..12977)
                     /locus_tag="OO544_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
     gene            13304..13699
                     /gene="rpsF"
                     /locus_tag="OO544_002453"
     CDS             13304..13699
                     /gene="rpsF"
                     /locus_tag="OO544_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HCR6768029.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            13706..14020
                     /gene="priB"
                     /locus_tag="OO544_002454"
     CDS             13706..14020
                     /gene="priB"
                     /locus_tag="OO544_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HCR6768030.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            14025..14252
                     /gene="rpsR"
                     /locus_tag="OO544_002455"
     CDS             14025..14252
                     /gene="rpsR"
                     /locus_tag="OO544_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HCR6768031.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            14294..14743
                     /gene="rplI"
                     /locus_tag="OO544_002456"
     CDS             14294..14743
                     /gene="rplI"
                     /locus_tag="OO544_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HCR6768032.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(14855..15826)
                     /locus_tag="OO544_002457"
     CDS             complement(14855..15826)
                     /locus_tag="OO544_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HCR6768033.1"
                     /translation="MKIKVSAGIIGAVLMLSASQSWAVTLKLSHNQDKSHPVHKAMEF
                     FAKKNKEYSNGDITIRIYPNGTLGTQRETMELIRSGAIPLVKTNAAEMEAFENSYKLF
                     SLPYLFRDRDHYYQVMQGDIGRKILDSTKSKGYFGLTFYDGGARSFYGNKPVLKPDDL
                     KGMKVRVQPSPGAVEMIKVMGGNPTPLDYGELYTALQQGVVDMAENSVMALTTMRHGE
                     VAKSFSLDEHTMVPDVVLMSNAAFDKLSPENQAVILKAAKESMSYMKDLWSEEEKQEF
                     AKLDKMGVKVYQVDKAPFIEKVQPMYANFAKDNPALAPMLADIQAAK"
     gene            complement(15879..>16295)
                     /locus_tag="OO544_002458"
     CDS             complement(15879..>16295)
                     /locus_tag="OO544_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HCR6768034.1"
                     /translation="SWAMSNADVPFLILDLLNTISDNPIIILLIINIILLIIGTFMDM
                     TPAVLIFTPIFLPVVTELGMDPIHFGIVMVLNMCIGICTPPVGSVLFVGCSVSKLPIN
                     KIIKPMLPFYAVMVLVLAMVTYIPQISMALPKALGY"
BASE COUNT         3826 a         3981 c         4334 g         4154 t
ORIGIN      
        1 tggtagaccg aataatcctt ttgaaccgcg tcgcaatcgc tacgcacaaa aatacttaac
       61 gttggcgcta atgggcggtg ccgctttttt cgtattgaaa ggttgtggcg atagtagcga
      121 tgtcgataac gacggcgacg gaacgtttta cgcgacagtg caggattgta ttgatgacgg
      181 taataatgcc gacatttgcg cgcgtggctg gaacaacgcc aaaacggcat tttatgccga
      241 tgttccgaag aatatgactc agcagaactg tcagtctaag tacgaaaatt cctactacga
      301 caatgttgaa cagagttgga tcccggtcgt ttctggattt ttgttaagcc gggttattcg
      361 taaagatcgc gatgagccgt tcgtctataa cagcggcgga tcctcatttg cttcccgccc
      421 cgtctggcgc aacacttccg gtgattactc ctggcgctcc ggttctggca aaaaagagtc
      481 ttactcttca ggcggcttta ccaccaaaaa agcgtcaacc gtttcgcgcg gcggctatgg
      541 tcgttcttcc agcgcccgtg ggcattgggg aggctaatca tgctgagaca caacgttcct
      601 gtgcggcggg atctggacca aattgccgct gacaacggtt tcgactttca tatcatcgac
      661 aatgaaatct attgggacga gagtcgggct taccgtttta ctctgcgcca gattgaagag
      721 cagatcgaaa aaccaactgc ggaactgcat cagatgtgcc ttgaggtggt ggatcgcgcg
      781 gttaaagacg aagagatcct gacgcaactg gcgatcccgc cgttgtactg ggatgtgatc
      841 gccgaaagct ggcacgccag cgatccttcg ctgtatggcc ggatggattt tgcctggtgt
      901 ggcaatgcgc cggtgaagct gctggagtac aacgccgata cgccgacttc gttgtatgag
      961 tcggcttatt tccagtggtt gtggctggag gatgcccggc gcagcggcat tattccgcgt
     1021 gatgccgatc agtacaatgc cattcaggaa cgcctgattt cacgctttag cgagctttat
     1081 agccgggagc cgttttattt ctgctgctgt caggacaccg atgaagacag gactaccgtg
     1141 ctgtacttgc aggactgcgc ccagcaggca gggcaggagt cgcggtttat ctacattgaa
     1201 gatctcggtt tgggcgtcgg cggtgtactg accgatcttg atgataatgt catccagcgt
     1261 gcatttaagc tgtatccgct ggagtggatg atgcgtgacg ataacggtcc gctgctgtgc
     1321 aagcgtcgcg agcaatgggt ggagccgtta tggaaaagta tcttgagtaa taaagggcta
     1381 atgccgctgc tttggcgctt cttccctggt catcctaacc ttcttgcatc ctggtttgag
     1441 ggtgaaaaat cgcagattgc cgctggcgaa agctatgtgc gtaaaccgat ctactcgcgc
     1501 gaaggcggaa acgtcaccat tttcgacggt cagaataacg tcgttgacca cgctgatggt
     1561 gattacgccg atgaaccgat gatctaccag gcgtttcaac ctctgccgcg atttggcgat
     1621 agctacacac tcatcgatag ctggattgtc gatgatgaag cgtgcggaat ggggatccgt
     1681 gaagataaca cactgatcac caaagacacc tcacgtttcg ttccgcatta cattgctgga
     1741 taagaatgtt ttagcaatct ctttctgtca tgaatccatg gcagtggcca tactaatggt
     1801 gactgccatt gatggaggga gacacagtgc actggcaaac tcacaccgtt tttaatcaac
     1861 ctataccatt aaataacagc aacttatacc tgtctgatgg cgcgctttgc gaagcggtaa
     1921 cgcgtgaagg tgctggctgg gatagcgatt ttctagccag tattggtcag cagttaggaa
     1981 cggctgaatc ccttgaactg gggcggctgg cgaatgtaaa cccgcctgaa ctgttgcgct
     2041 acgatgccca ggggcggcgt ctggacgatg ttcgtttcca tcccgcatgg cacctgctga
     2101 tgcaggtgtt atgtaccaat cgggtgcaca atcttgcctg ggaagaagac gctcgctccg
     2161 gcgcatttgt ggcgcgcgcg gcgcgtttta tgttacatgc gcaggttgag gctgggtcgt
     2221 tatgtccaat aacgatgacc tttgccgcca cgccattgtt gttacagatg ttacccacgc
     2281 cgtttcagga ctggaccacg ccgctgctga gcgatcgcta cgattctcac ttattgccag
     2341 gtgggcaaaa acgcggtttg ttgattggca tgggaatgac ggaaaagcag ggtggttccg
     2401 atgtcatgag caacaccacc cgtgcagagc gtttggaaga tggctcttat cggctggtgg
     2461 ggcataaatg gtttttctcg gttccgcaaa gcgatgcgca tctggtgctg gcgcagacca
     2521 cgggcggtct gtcctgtttt tttgtgccgc gctttttgcc tgacgggcaa cgcaacgcga
     2581 ttcgcctcga acggctgaaa gataagctgg gtaatcgctc taacgccagt tgtgaagtgg
     2641 agtttcagga tgccattggc tggttattgg ggcaggaagg ggaaggaatt cgtctgatcc
     2701 tgaaaatggg ggggatgacg cgttttgatt gcgccctggg tagccatgcc atgatgcgcc
     2761 gtgcgttttc gctggcgatt tatcatgcac atcaacgcca tgtttttggt aatccattga
     2821 tccaacagcc ccttatgcgt catgtcttaa gtcgcatggc acttcagctt gaagggcaaa
     2881 cggcgttgct gtttcgtctt gcgcgagcgt gggaccggcg cgccgatgcc aaagaagccc
     2941 tgtgggcgcg tttatttacg cctgcggcaa aatttgtgat ctgcaaacga ggtatgccgt
     3001 ttgtggccga agcgatggag gtgctgggcg gcattggtta ttgcgaggag agcgagctgc
     3061 cgcggcttta ccgggagatg ccggtaaaca gtatttggga aggttccggc aatattatgt
     3121 gcctggatgt gttgcgcgtt ctcaataagc aagcgggcgt atacgactta ttgtcggaag
     3181 catttgtgga agtgaaaggg caggatcgct attttgatcg cgcggttcgt cgtttacagc
     3241 agcagctgcg taagccagct gaagaactgg ggcgagagat tactcatcag ctattcctgc
     3301 tgggctgcgg tgcgcaaatg ttgaaatatg cttctccgcc aatggcgcag gcgtggtgtc
     3361 aggtgatgtt agatacgcgc ggcggcgtac ggttgtcaga gcagatccag aatgatttat
     3421 tgctgcgggc gacgggggga gtgtgtttgt aaacgtatac gactgatgcg acgctggttt
     3481 cgattaactc aatgaaatat gtgaaaattg taggccggac aaggcgctcg cgccgcatcc
     3541 ggcattgttc atttgctgta gatgacactc atgcatacag tatcgcctgt acgcgccagt
     3601 tatcaggctg ctggtcttcg tacatggtaa taatgcggta ccatgacgca ccttgttcat
     3661 cggcctttaa cgccacgata cgttccacgt cctgcggatc ccctgaaata ttgctaactg
     3721 agatctgacc tatttcattc aggcccgtca cgcagtcagc actggcgaat tctgcagact
     3781 gtgccgtggc gctcgcagga taagtcacta attgcagcga gggtgaggca agtaattgtt
     3841 tcatcgtcag ctccattttt cgtttcccca catgttgcag ggcattccat cgcggtaata
     3901 aacgcccagg cttccagccg gtaatttatt gtgcacaggt aaacgcatag ggacgcaaag
     3961 caatgtaaag ctgactatgt aagttgtctg atttatttac ggtacaaaat ggcttgtgaa
     4021 taccactgtc ctgtcacgat ggtttcgtcc accatgacga caacgtaata atcagctttt
     4081 gcagcgacag cttttgctct gatttctgct aatgcgtcat ccggagaacc ccgaaccatc
     4141 gtgcttacgc tacctattcg ctgtaaccct tgcgtctggt cgcggcgaat ctcttgcgga
     4201 tggtccgtta ctggcggtgc tggctgtggc gtaccttgca gtgcgctaca ggcacttaac
     4261 atcaacacca ataataaact ggcaaaccgg taaataacgc tattacgttt cctgctaacc
     4321 atagtgtagt gccttattaa tttaactttg ggggaatgtt cccgaattgt tacctggtac
     4381 gcgattttcg aatgaaaacg tgacgaaagc gtaacccaca tctcaacatt atgaactaat
     4441 accgccagca gaaacttgtg ctaaggattt cttcatgtca taccagacaa aaaggagaga
     4501 cagatgattg aaatagaatc acgcgagctg gcagatattc ccgttcttca tgcttatcct
     4561 gtcgggcaaa aagatacccc gttaccgtgc gtaatttttt atcacggctt tacttcatcc
     4621 agcctggtgt atagctattt tgccgttgcg ctggcgcagg ctggtttgcg ggtgatcatg
     4681 ccggatgcgc ccgatcacgg tagccgtttt agtggcgacg aagcgcggcg gttaaatcaa
     4741 ttctggcaaa tcttgctaca aagtatgcag gaattcacta ctttacgtgc ggcaatagcc
     4801 gaagaaaact ggctgcttga tgaccgtctg gcagtcggtg gcgcgtcgat gggcgcgatg
     4861 acggcactgg gaattaccgc tcgccacccc acggtgaaat gtaccgccag catgatggga
     4921 tcgggctatt ttacatcgct cgcccgttca ctgtttccac cgctgatacc tgaaacggca
     4981 gcacagcaga atgaattcaa taacattatc gcgccgctgg cagagtggga agcgacaaac
     5041 cacctggaac aacttgggga cagacctcta ctgctgtggc atggcctcga cgatgacgtt
     5101 gtgcctgccg acgaatcact acgtttgcag caggccttaa gcgagacggg acgggataaa
     5161 ctgctaacct gttcatggca gccaggcgtg cgtcaccgca ttacgcctga ggcgttagat
     5221 gctgccgtga catttttccg ccagcatctt taaacacgca gaatgctgac cccttgcgct
     5281 tccagttgtt gcaggatttc cgggttagca tttttgccgg tgataagcat atcgatttga
     5341 tcggcacggc taaaaagcat tcccgcgcgt tcgccaatct tactgctatc aaccagtacc
     5401 accagtttcc ctaccacgct cagcatcttc tgctctgcca ttgctgtcag catatcggtt
     5461 ttatacaacc cttctgcggt cagccctttt ccgctggtaa acatccagtg cccggcatag
     5521 agactgtttt cgctgccctg cgggcttaaa gtgatggact gacttttgtt gtactgtcca
     5581 cccataatga tcacgctgtc atgttcttga tcgatcaggt aatttgccag cggtagataa
     5641 ttagtgatga tttgcactgg cttgccacac atttcccgcc caagcagaaa cgcggtggag
     5701 ccgcagttga tgactacgct ttcgcccgga ttaaccagct gcgaggccgc tttagcaata
     5761 cgtacttttt catcgtgatt ctgcgcctga tgcagattca tcggcgtcca gcgcgggcgc
     5821 tgttgggtaa tagcttctgc gccattgcgc acctttttca gtttgccgcg ttcgtcaagt
     5881 ttattgatat cgcgtcgcgc agtggcaggc gaaattccca gacgctcaac gactttctca
     5941 acggtcacaa agcccaattg tgcgagcatt tccaggagga tttgatgtct ttgtgcttca
     6001 gtcatgagct attccgataa aatttgattt ttttagatga tatttgaaat agctaggaaa
     6061 tactacgccg gatagcgcga acttctccac gctatccggt ataaacgaca gattacagga
     6121 atgacttgaa cggcagatcc ggttcaatag tgaagcaatc atcgaaaccg cgcggatagt
     6181 ggtactcgaa gttgtcttta tccagcggcc aggtaaattt gccgccaacc tgccagataa
     6241 acggcttgaa gccatacttc agacgatctt ttttcatctc ccacagcacg cggatctctt
     6301 gcggatcggc ctggaagttt gaccagatgt cgtggtggaa cgggataacc actttcgcat
     6361 tcagggcttc acccatacgc agcatatcgg cgctggtcat tttgtcggta ataccgcgcg
     6421 ggttctcacc atacgatcct aacgccacat cgatctgatg ttcgttaccg tgcttcgcat
     6481 aatagttaga gtagtgggaa tcgccactgt gatataggga accgccaggg gttttgaaca
     6541 ggtagtttac tgcgcgatcg tccatgccat ctggcagtac gccagccgct ttttgatcgg
     6601 caggcagggt gatcagtgca gtacggtcga aagcgtcaag cgcatgaatt tcaatgtctt
     6661 tcacttttac aacatcgccc ggtttgacca cgatgcaacg ctctttcggt acgccccagc
     6721 caatccacaa atcaacgcag gttttcggtc caatgaatgg tacgtcatca gcacagttct
     6781 gcatcacggc agcagcgacg ttaacgtcga tatgatcgtt gtgatcgtga gtcgccagta
     6841 ccgcgtcgat ctggcgaatc gcaaacggat caagaacaaa cggggtggtg cgtaggtttg
     6901 gctgcagttt tttcacgcca gccatgcgct gcatctggtg accctgtttc attaacgggt
     6961 taccgtgact ttgtttgcca gtgccgcacc agaaatcaac gcaaacgttg gtgccacctt
     7021 ctgatttcaa ccagatcccg gtgcagccta gccaccacat cgcaaatgtg ccaggggcga
     7081 cctgctcttg ttcaatttct tcattcagcc agctacccca ctccgggaaa gtgctcagga
     7141 tccaggattc tcgggtgata cttttcactt tactcatcgc catttacctt catgatagtt
     7201 caattcgaat caatatgtga ttggttttga ttaatcctga cactattttt tcaggaaggc
     7261 aatgaccatt ttttgacttt ttgccaggga agttgttgtt gattttgagt gtggaaagat
     7321 ttaatggaat gtgtagttaa attaactaaa tgaatttaaa taaataattg tttcgttgtg
     7381 tgaatcccac tctatccgtg tggaattatt tgcgggtcgc gtcacattta atcataaata
     7441 atcttgttgt gattactttt gaaaattaga gtgagtgcac aacattccgg gtgtgcggaa
     7501 tacccggtta cctcttcttc aggagatcgt tatggagatc ctctacaaca tctttaccgt
     7561 gttttttaac caggtcatga ccaatgcccc gttgttgctg ggtattgtga cctgtctggg
     7621 ctacatccta ctgcgcaaaa gtgtcagcgt tattattaaa ggcacgatta aaaccataat
     7681 tggtttcatg ttgttgcagg cagggtccgg catcctcacc agcaccttca aaccggtggt
     7741 ggcgaaaatg tccgaagtct acggcattaa acggcgcaat ttccgatact tacgcgtcga
     7801 tgatggcaac catcgaccgc atgggcgatg cctatagctg ggtaggttac gcggtgttgt
     7861 tagcgctggc gctgaacatc tgttacgtgc tgctgcgtcg cattaccggt attcgtacca
     7921 tcatgctgac tggtcatatc atgttccagc aggcggggct gattgccgtt acgttgttta
     7981 ttttcggcta ctccatgtgg acaaccatta tctgcacggc gattctggtt tcgctctact
     8041 ggggcattac ctccaacatg atgtacaagc cgactcagga agtgacggac ggctgcggtt
     8101 tctccatcgg tcaccagcaa cagtttgcat catggactgc ctataaagtt gcgccgttcc
     8161 tcggcaaaaa agaggaaagc gttgaagacc tcaaactgcc aggctggctg aatattttcc
     8221 acgacaacat cgtctccacg gcgattgtca tgaccatctt ctttggtgcc attctgctct
     8281 ccttcggtat cgacaccgtg caggcgatgg caggcaaagt gcactggacg gtctatatcc
     8341 tgcaaaccgg tttttccttc gcggtggcga tcttcatcat cactcagggt gtgcgcatgt
     8401 ttgtggcgga actctctgaa gcgtttaacg gtatctccca gcgcctgatc cctggtgcgg
     8461 ttctggcgat tgactgtgcg gctatttata gcttcgcgcc gaacgccgtg gtctggggct
     8521 ttatgtgggg caccatcggt cagctgattg cggttggcat cctggtcgcc tgcggttcct
     8581 cgagcctgat tatcccaggc tttatcccga tgttcttctc caacgccacc atcggcgtgt
     8641 tcgctaacca cttcggcggc tggcgtgcgg cgctgaagat ttgtctggtg atggggatga
     8701 tcgaaatctt tggttgcgtc tgggcggtga aacttaccgg tatgagtgcc tggatgggga
     8761 tggcggactg gtcgattctg gcaccgccga tgatgcaggg cttcttctcc atcggtatcg
     8821 cctttatggc cgtcatcatt gtaattgcac tggcttatat gttcttcgct ggccgcgcac
     8881 tgcgcgcaga agaagatgca gaaaaacaac tggcagaaca gtctgcttaa taaggagttt
     8941 tgattatgac cgtacgtatt ctggctgtgt gtggcaacgg acaaggcagt tccatgatca
     9001 tgaggatgaa agtggaccag tttttaaccc aatcaaacat tgaccacacg gtaaatagct
     9061 gcgcggttgg cgaatacaaa agcgagttga gcggcgcgga tatcatcatc gcttctacgc
     9121 acattgcggg cgaaatcacc gtgaccggca acaaatacgt ggttggcgtg cgcaacatgc
     9181 tctctcctgc cgactttggc ccgaaactgc tggaagtgat caaagcgcat ttcccgcagg
     9241 atgtgaagta aggacgcgcc atgaaattac gtgattcgct ggcggagaat aaatccatcc
     9301 gcctgcaggc tgaagctgag acatggcagg acgcagtaaa aatcggcgtt gacctgctgg
     9361 tggcggcgga tgttgtcgag ccgcgttact accaggcgat tctggatgcg gttgaacagc
     9421 acggtccgta ctttgtgctc gcaccaggcc tggcaatgcc gcacggtcgc ccggaagagg
     9481 gcgttaagaa aaccggtttt gcgctggtga cgctgaaaaa gccgctggag ttcaaccacg
     9541 aagataacga cccggtggat atcctcatca ccatggcggc ggtcgatgcc aacacgcacc
     9601 aggaagtggg catcatgcag atcgtcaacc tgtttgaaga tgaagaaaat ttcgaccgtt
     9661 tacgtgcctg ccgtaccgag caggaagtac tggatctcat tgaccgcacc aacgcggcag
     9721 cttaagaagg aattgaagat gtcattaccg atgttgcaag tcgcgctgga caaccagact
     9781 atggatagcg cctacgaaac cactcgcctg attgccgaag aagtcgacat tatcgaagtg
     9841 ggtaccattc tgtgcgtggg cgaaggcgtg cgtgcggttc gcgatctgaa agcgctctac
     9901 ccgcacaaaa tcgtgctggc agacgccaaa attgccgatg cgggcaaaat tctttctcgt
     9961 atgtgcttcg aagccaacgc tgactgggtg accgtgattt gttgtgcgga tatcaacacc
    10021 gccaaaggtg cgctggacgt agcaaaagag tttaacggcg acgtgcagat cgaactgacc
    10081 ggttactgga cctgggaaca ggcgcaacag tggcgcgacg caggtattca gcaggtggtt
    10141 tatcaccgca gccgtgacgc gcaggccgct ggcgtggcgt ggggtgaagc cgatatcact
    10201 gcgatcaaac gtctttccga tatgggcttc aaagtcaccg tcaccggtgg cctggcgctg
    10261 gaagatctgc cgctgttcaa aggcattcca attcatgtgt ttatcgccgg tcgcagcatc
    10321 cgtgatgccg cttctccggt tgaagccgcg cgtcagttca aacgttccat cgccgaactg
    10381 tggggctaag gagcggatat gttgtccaaa caaatcccgc ttggcatcta tgaaaaagcg
    10441 ctccccgccg gggagtgctg gctggaacgc ctgcgactgg caaaaacgtt gggcttcgat
    10501 tttgtcgaga tgtcggtaga tgaaactgac gagcgcctgt cgcgcctcga ctggagccgc
    10561 gagcagcgtc tggcgctggt gaatgcgatt gttgaaaccg gcgtgcgtgt gccgtccatg
    10621 tgcctttctg cccatcgtcg tttcccgttg ggcagcgaag atgacgcggt gcgggcgcag
    10681 gggctggaga ttatgcgtaa agccatccag ttcgcccagg acgttggcat tcgcgtgatc
    10741 cagctggcgg gctatgacgt ttactatcag gaagccaata acgaaacgcg tcgtcgtttc
    10801 cgtgacggcc tgaaagagag cgttgagatg gcgagccgcg cgcaggtgac gctggcgatg
    10861 gagatcatgg attatccgtt gatgaactcc atcagcaagg cgctgggtta cgcgcactat
    10921 ctcaacaacc cgtggttcca gctctacccg gatatcggca acctgtcggc gtgggacaac
    10981 gatgtgcaga tggagttgca ggccggaatc gggcatatcg tcgcggtgca tgtgaaagac
    11041 accaaaccgg gcgtcttcaa aaacgtgcca tttggcgaag gtgtggtgga tttcgaacgt
    11101 tgcttcgaaa cgctcaaaca gagtggctat tgcgggccgt acctgattga gatgtggagc
    11161 gaaacggcgg aagacccggc ggcagaagtg gtgaaagcgc gtgattgggt gaaagcgcgc
    11221 atggccaaag caggcacggt ggaggcggca taatgcaaaa gctaaaacag caggtgtttg
    11281 aagccaacat ggatctgccg cgctatgggc tggtgacctt tacctggggc aacgtcagcg
    11341 ctatcgaccg cgaacgcggg ctggtggtga tcaagcccag cggcgttgcc tacgaaacca
    11401 tgaaagcaga cgatatggtg gtggtcgata tgagcggcaa ggtggtggaa ggggagtatc
    11461 gcccgtcttc cgacaccgcg acgtatctcg aactctaccg tcgttacccg tcgcttggcg
    11521 gcattgtcca tacccactcc actcatgcta ctgcatgggc gcaggcgggg ctggcgatcc
    11581 cggcgttagg cactacgcac gccgactact tctttggcga catcccgtgt acgcgcggat
    11641 taagcgaaga agaggtgcag ggcgagtatg aactgaacac cggcaaagtg attatcgaaa
    11701 cgctgggtga cgccgagccg ctgcatacgc cggggattgt ggtgtatcag cacggtccgt
    11761 tcgcctgggg gaaagatgcc cacgacgcgg tgcataacgc ggtggtgatg gaagaagtgg
    11821 cgaaaatggc gtggattgcg cgcagtatta acccacaact gaatcacatc gacagcttcc
    11881 tcatgaataa acacttcatg cgtaagcacg gtcctaacgc ctattacggg cagaagtaag
    11941 cagtgcgccc cggaattatt acctcctgcg ggatgtaatt ggtctgattc cggggccgct
    12001 tcagttaaaa aatcgtataa acaacaggtg tttttatggc tatcaatgga ttgtttgtca
    12061 gaacggatcc gcaacgtcgc cgctatggcg ttgcgttact tgtgggtgta ctttcaggaa
    12121 ttatttcagc atttgttaaa tggggagcgg aagtcccctt gccaccgcgc acattttccg
    12181 gtggtcgcga tgaatttaac ccgccgtata tctttctacg ggattattta ggtatcgacc
    12241 caacccaaac tgtgtataca ttctcggaac atattatcaa cccggtgatg gtgacgcata
    12301 tcatcttctc actggttttt gcgatagggt attgtattgt cgcggaaatt ttcccgaaag
    12361 tgaaattatg gcaggggata ttagcagggc ttattgtgac agtggcagtc cacggtatat
    12421 tttgcccggc gctgaattta acgccgccgc tcactcaatt gccttttgac gaatatgcct
    12481 ctgaaatcct ggggcatatt ttctggttct ggataataga aattatgcgc cgcgacttac
    12541 gtaatcgcat tactcatgag ccagatccgg aagtcgcaat ccggtgaata accgcataaa
    12601 aaagccccct gcgaaggggg caaagcaaac tatggcaatg tttcgttggt tatacctggt
    12661 gctagcgata aatatccgcg ctggcgtgca tattgccgtt actccccggt tcccgcatca
    12721 gaattacgtg gtagtacgtt actccccggt tcccgcatca gaattacgtg gtagtacgtt
    12781 gcgccctgcg catctgtctc ttcatttaat gcctgacgcg cttcgctttc agtggcgaaa
    12841 ttatgattga tataaatcac gcctaagctt tgcacatcat ccatgtttct ggcctggtgg
    12901 ttattaattt caatggctgc ccatgtattt gcacttagca aaagcacagc cagaagggct
    12961 aaaactcgac tgaacataga tacctcctcg acggctgact ttgtgtgctc tcctctgtga
    13021 tgatcttctg atttaatttt aatcaatgat aaagaagttg atggtgacca tttctgatgc
    13081 agttgttcaa aaaaacacca tgatgaagtg tgatgaactc caaatcagcg gtttagaggt
    13141 taattgcgaa aggggagatt tatttcggct ctgcccttga gtttagcggg gcatacaagt
    13201 actataacgc gtcatttttc agccgacctt taacacgttc cttgcctccc cgggattcgg
    13261 ctgacccaga caggaggctg aataatccgt aaggagcaat tcgatgcgtc attacgaaat
    13321 cgtttttatg gtccatcctg accagagcga acaggttccg ggcatgatcg agcgctacac
    13381 tgctgccatc actggtgcag aaggcaagat ccaccgtctg gaagactggg gccgccgtca
    13441 gctggcttac ccgatcaaca aactgcacaa agcacactac gttctgatga acgttgaagc
    13501 tccgcaggaa gtgatcgatg agctggaaac taccttccgc ttcaacgatg ccgttatccg
    13561 cagcatggtt atgcgtacca agcacgctgt taccgaagca tctccgatgg ttaaagcgaa
    13621 agacgagcgc cgtgagcgtc gcgatgattt cgcaaacgaa accgctgatg atgctgaagc
    13681 tggggattct gaagagtaat ttctgatgac caaccgtctg gtgttgtccg gcaccgtgtg
    13741 caggactccc cttcgaaagg tcagtccatc aggaattcct cactgccagt tcgtgcttga
    13801 gcatcgttct gtgcaggagg aagccggttt tcaccggcag gcgtggtgcc aaatgcccgt
    13861 tattgttagc ggacacgaaa accaggccat tactcacagt ataacggtcg gcagtcgcat
    13921 aaccgttcag gggttcattt catgccacaa ggcaaagaac ggactgagca aaatggtttt
    13981 gcatgccgag cagattgaat tgatagattc tggagactag ccatatggca cgttatttcc
    14041 gtcgtcgcaa gttctgccgt ttcaccgcgg aaggcgttca agagatcgac tataaagata
    14101 tcgctacgct gaaaaactac atcaccgaaa gcggtaagat tgtcccaagc cgtatcaccg
    14161 gtacccgtgc aaaataccag cgtcagctgg ctcgcgctat caaacgcgct cgctacctgt
    14221 ccctgctgcc gtacactgat cgccatcagt aatcggtcac ggtccattaa tacgactttg
    14281 agaggataag gtaatgcaag ttattctgct tgataaagta gcaaacctgg gtagcctggg
    14341 tgatcaggta aacgttaaag cgggctatgc tcgtaacttc ctggtaccgc agggtaaagc
    14401 tgttccagct accaagaaaa acattgaatt cttcgaagca cgtcgcgctg aactggaagc
    14461 taaactggct gaagttctgg cagctgctaa tgctcgcgct gagaaaatca atgcactgga
    14521 aactgttacc atcgcgtcta aagctggcga cgaaggtaaa ctgttcggtt ccatcggtac
    14581 tcgcgacatc gctgacgctg taactgcagc tggcgttgaa gtggctaaga gcgaagttcg
    14641 tctgccgaac ggcgttctgc gtaccactgg cgagcacgaa gtgagcttcc aggttcacag
    14701 cgaagtattc gcgaaagtga tcgtaaacgt agtagctgaa taattcgtta ttcaacgaga
    14761 cgtaaaaacg ccgaccagtg gtcggcgttt tgcttttgta gcgagcactt gcagatatcg
    14821 ataagggcca caataagcag ccctacgaag ataattactt agctgcctga atatcagcca
    14881 gcattggggc aagggctggg ttgtccttag cgaagtttgc gtacattggc tgtactttct
    14941 cgataaacgg agctttatct acctggtaga ctttcacgcc cattttatcc agttttgcaa
    15001 attcttgttt ctcttcctcg ctccacaagt ctttcatgta gctcattgat tctttagctg
    15061 cttttaatat aactgcctga ttttccgggc taagtttatc aaacgcagca ttgctcatca
    15121 gaaccacatc gggaaccata gtgtgttcgt caaggctgaa ggattttgcc acttcaccgt
    15181 gacgcatggt ggtcagcgcc atcacgctgt tttctgccat atcgaccaca ccctgctgta
    15241 aggctgtata caactcgccg taatccagtg gcgtcgggtt accgcccatg actttgatca
    15301 tttcaactgc gccagggctt ggctggacac ggactttcat gcctttcaga tcgtctggtt
    15361 tcagtactgg tttattgcca tagaaactgc gggcgcctcc atcataaaaa gtcagcccga
    15421 aataaccttt gcttttcgtt gagtcgagga tttttctccc gatatcgccc tgcatgacct
    15481 gataataatg atcacgatcg cggaacaaat aaggcaggct aaataattta taggaatttt
    15541 caaatgcttc catttctgcc gcattggttt ttaccagtgg aatagcgcca gaacgaatca
    15601 gctccattgt ttctcgttga gtacccaatg ttccatttgg ataaatacga atagtaatat
    15661 caccgttaga gtactctttg ttctttttcg caaagaactc catcgcttta tgaacaggat
    15721 gagacttatc ctgattatga ctcagtttta atgtcactgc ccaggactgg cttgcggata
    15781 acataagaac agcaccgata atgccagcgg atacttttat tttcatagat gttccttttc
    15841 taataaatat ggcggcaggt gccgccagtg aatgcgcatc agtagcccag tgcttttggc
    15901 aatgccatac tgatttgcgg aatataggtc accattgcca gcaccaatac catcacggca
    15961 tagaatggca gcataggttt gataatttta ttaatcggta atttagatac actacaaccg
    16021 acaaataaca cactgcctac aggcggagta caaataccga tacacatgtt cagtaccatg
    16081 acaataccaa agtgaatcgg gtccatgccc agttcggtga caacaggtaa aaatatcggc
    16141 gtaaaaatca gtaccgccgg tgtcatatcc ataaaagtgc cgataatcaa caggataata
    16201 ttgataataa gcaggattat tattgggttg tcagaaatag tgttaagcaa atcgagaatc
    16261 aggaagggaa cgtcagcgtt tgacattgcc catga
//
LOCUS       DAJTZL010000088        16189 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.088, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000088 DAJTZL010000000
VERSION     DAJTZL010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16189
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.088"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            78..2393
                     /gene="bisC"
                     /locus_tag="OO544_002459"
     CDS             78..2393
                     /gene="bisC"
                     /locus_tag="OO544_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HCR6768035.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKTAEWAAEICGVGAAKIRQLAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFTRRSAVLSSMQGSLPGGCDAVDKIPVARI
                     VEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPELVVISEC
                     FWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDFDVFAEL
                     SERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVELPPFAEFWQANQLIEMPEN
                     PDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYGYPDCPGHPMWLEPDEWQGN
                     AEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGITEGDMVRV
                     WNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDLPSSRLG
                     NGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(2362..2802)
                     /locus_tag="OO544_002460"
     CDS             complement(2362..2802)
                     /locus_tag="OO544_002460"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR6768036.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(2799..3362)
                     /gene="tag"
                     /locus_tag="OO544_002461"
     CDS             complement(2799..3362)
                     /gene="tag"
                     /locus_tag="OO544_002461"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HCR6768037.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            3520..4218
                     /locus_tag="OO544_002462"
     CDS             3520..4218
                     /locus_tag="OO544_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR6768038.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPNYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTQTDPLWHASVSTLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            4447..5655
                     /locus_tag="OO544_002463"
     CDS             4447..5655
                     /locus_tag="OO544_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6768039.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFCVKRVTMASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            5979..7670
                     /gene="eptB"
                     /locus_tag="OO544_002464"
     CDS             5979..7670
                     /gene="eptB"
                     /locus_tag="OO544_002464"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HCR6768040.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDETYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKEAAAN"
     gene            7762..7838
                     /locus_tag="OO544_002465"
     tRNA            7762..7838
                     /locus_tag="OO544_002465"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7796..7798,aa:Pro,seq:cgg)
     gene            7873..8006
                     /locus_tag="OO544_002466"
     ncRNA           7873..8006
                     /ncRNA_class="other"
                     /locus_tag="OO544_002466"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            8751..10358
                     /gene="dppA"
                     /locus_tag="OO544_002467"
     CDS             8751..10358
                     /gene="dppA"
                     /locus_tag="OO544_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HCR6768041.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTRELNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQTPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            10666..11685
                     /gene="dppB"
                     /locus_tag="OO544_002468"
     CDS             10666..11685
                     /gene="dppB"
                     /locus_tag="OO544_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HCR6768042.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            11695..12597
                     /gene="dppC"
                     /locus_tag="OO544_002469"
     CDS             11695..12597
                     /gene="dppC"
                     /locus_tag="OO544_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HCR6768043.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYIVIVLF
                     ITIFANWIAPYNPADQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            12608..13591
                     /gene="dppD"
                     /locus_tag="OO544_002470"
     CDS             12608..13591
                     /gene="dppD"
                     /locus_tag="OO544_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HCR6768044.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLVFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            13588..14592
                     /gene="dppF"
                     /locus_tag="OO544_002471"
     CDS             13588..14592
                     /gene="dppF"
                     /locus_tag="OO544_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP binding subunit
                     DppF"
                     /protein_id="HCR6768045.1"
                     /translation="MSTQEATLQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLSGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(14622..15893)
                     /locus_tag="OO544_002472"
     CDS             complement(14622..15893)
                     /locus_tag="OO544_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCR6768046.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRVLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
BASE COUNT         3846 a         4040 c         4348 g         3955 t
ORIGIN      
        1 ttattcctgg gaatactcgg aaatttgtaa gtaatattta actgctcaat acatctaacc
       61 tttcaggagt cttcggtttg gccaactcat cctcacgata ttccgttctg actgccgccc
      121 actgggggcc catgctggtt gaaaccgacg gcgaaaccgt gtttagctcg cgtggcgcgt
      181 tagccacagg aatggaaaac tccttgcaga gcgcggttcg cgaccaggtt cacagcaata
      241 cgcgggtacg atttccaatg gtgcgcaaag gctttctcgc gtcaccggaa aacccgcaag
      301 gcattcgtgg gcaggatgaa tttgttcgcg tgagttggga tgaggcgctg gatcttattc
      361 accaacaaca taaacgcatt cgtgaggctt atggtccggc atcgattttt gctggttcct
      421 acggctggcg ttcaaacggc gtgctgcata aggcctcgac attattacaa cgctatatgg
      481 cgctggcagg cggttatacc gggcatctgg gggattattc gaccggtgcg gcacaggcga
      541 tcatgccgta tgtcgtaggt ggcagcgaag tttatcaaca gcaaaccagt tggccgctgg
      601 tgctggaaca tagcgatgtc gtggtgttgt ggagtgctaa cccactcaat acgctgaaaa
      661 ttgcgtggaa tgcatccgat gagcaggggc tttcttactt ttccgcactg cgtgacagtg
      721 ggaaaaagct gatctgcatt gatccaatgc gatcggaaac cgtcgatttc tttggcgata
      781 aaatggagtg ggtggcaccg cacatgggca ccgatgttgc gctgatgctg gggatcgccc
      841 atacgctggt ggaaaatggt tggcacgacg aagcgtttct ggcgcgttgc accacaggtt
      901 atgccgtctt cgcctcttat ttgctgggcg agagtgacgg aatagcgaaa accgccgaat
      961 gggcagcaga gatttgtggt gttggcgcag cgaaaatccg ccagctggcg gctattttcc
     1021 accaaaatac caccatgctg atggcaggct ggggaatgca gcgccaacag tttggtgagc
     1081 aaaaacactg gatgatcgtc acgctggcag caatgttggg gcaaatcggc acacccggcg
     1141 gcggttttgg tctttcttac cattttacgc ggcgttctgc ggtgctctct tccatgcagg
     1201 gcagcttgcc gggtggctgc gatgcggtgg ataaaatccc tgttgcccgc attgttgaag
     1261 cactggaaaa ccctggtggc gcatatcaac acaacggtat gaaccgacat ttcccggata
     1321 ttcgttttat ctggtgggcg ggcggtgcca actttactca tcatcaggat accaatcgtc
     1381 tgatccgtgc ctggcaaaaa ccggagctgg tggtgatctc tgaatgcttc tggacggcgg
     1441 cggcgaaaca cgcggatatc gttctgcctg cgactacctc ttttgagcgt aatgatctca
     1501 ccatgaccgg tgattacagt aatcagcatc tggtgccgat gaagcaagtg gtgccgccac
     1561 gctatgaagc gcgtaatgat tttgatgttt ttgccgagtt gagtgaacgt tgggagaagg
     1621 gcggctatgc gcggtttacg gaaggaaaaa gtcagctgca atggctggaa acgttttata
     1681 acgttgcccg gcagcgcggg gcaagccagc aggttgaatt gccgccattt gctgagttct
     1741 ggcaagccaa ccagttaatt gagatgccgg aaaacccgga cagcgagcgg tttattcgct
     1801 tcgctgattt ttgccgcgat ccgctggcgc atccgttaaa aaccgccagc ggcaagattg
     1861 aaatcttctc acagcgtatt gccgattacg gttacccgga ttgccctggg catccaatgt
     1921 ggctggagcc ggacgaatgg cagggcaatg ccgaaccaga acagttgcag gtactttctg
     1981 cccatccggc gcaccgcctg cacagccagc tgaattacag ttctctgcgc gaattgtacg
     2041 cggtagcaaa ccgtgagcct gtcaccattc atcctgacga tgctcaggcg cgcggcataa
     2101 ccgaagggga tatggtgcgg gtgtggaact cccgtgggca gatccttgcc ggagcggtca
     2161 ttagcgaggg aattaaacct ggcgtgattt gcattcacga aggggcatgg ccggatctgg
     2221 atttaaccgc tgacggtatt tgtaaaaacg gcgcagtgaa cgtgctgacc aaagatctcc
     2281 ccagctcgcg gctggggaat ggctgtgcgg gtaatacggc gctggcatgg ctggaaaaat
     2341 acaacggtcc ggaactgaca cttacagcgt ttgaaccacc ggccagctca taatccaggt
     2401 gggtagttgg gtttcatcct gccatgcgca atcgacaatg tgaaaaccct gcgcacggta
     2461 aaaatctatc gccggttgat ttttttgata aacctccagc atcagatggg gatagcgctg
     2521 ctgcacatac tgcatcagcg ccttaccaat accgcgtctg acggccttcg gtgcgacaaa
     2581 cattgctgcc agaaatcggc cttccataat gctgacaaaa ccgagaagct taccgtcctc
     2641 ttcccagacc cagttttgcg cgttggcaag ataggcatcc cgcaccagcg gaatgcagtc
     2701 acgccagtaa ttcgctttta taaagggatg cccccaggtt gtactttcca gccacagttc
     2761 gaggatcgcg gggagttctg aacgttgcgc ttcccgaatc atggtttatt tcccggatag
     2821 caacagcagc caaccacatg atcattcacc agcccacatg cctgcataaa ggagtaacag
     2881 attgtggtgc cgacaaactt aaaaccacgt tttttcagtg ctttagatag ggcgtcggag
     2941 gcggacgtag atgtgggaat ttcgctcaac gttgtggctt gtgtcacctg tggctgatga
     3001 tttacaaacg accagacaaa gtcggcaaac ggttcgccgt tctgttccat ttgcaggtac
     3061 gcccgcgcat taccaataat tgcctgaatt ttccctcgat ggcggataat cccggcgtcc
     3121 tgtaccagtc tttcgacatc ctcttcctgc attgctgcga ccttcaccgg atcgaactga
     3181 tgaaagcagg cgcgatagtt ttcgcgtttt ttgaggacgg tgatccacga taacccagcc
     3241 tgctgccctt caaggcagat catttcgaac agttttttac tgtcagtttc aggcacgccc
     3301 cattcattat catggtaggc aatataaagc gggtcctgac tcacccagcc gcaacgttcc
     3361 atactttccc tcgctatgta cccgatcgta aaaatttcag ccgcttttct tgacgcatcg
     3421 gtgaaaaagt caatatttaa tacagggcta ctatgcccga tatccttcat acaatgacaa
     3481 taatattgcc gaattcggct cttccctgga gtcgtgttaa tgatcataaa aaaaagcggt
     3541 ggtcgctggc agctaagcct gctggcgagc gtggtaatca gtgccttttt tctcaacaca
     3601 gcttacgcct ggcaacaaga atatatcgtt gatacgcaac ccggacattc cacagagcgt
     3661 tacacctggg atagtgatca tcaacctaat tacaacgata ttttgtcgca acgtattcaa
     3721 agtagccaaa gggcgctggg actggaagtc aatctggcgg aagaaactcc tgtggatgtg
     3781 accagcagta tgagtatggg ctggaatttt cctttatatg aacaggttac aaccggcccg
     3841 gtcgcggcat tacattacga tggcacaacc acctcgatgt ataacgagtt tggcgacagt
     3901 actaccacgc agaccgatcc gttatggcat gccagcgtga gtaccttagg ctggcgtgtt
     3961 gactcccggc ttggcgatct ccgaccctgg gcgcaaatca gctataacca gcaatttggc
     4021 gagaatatct ggaaggcgca atcaggcctg agccggatga cggcgacaaa ccagaacggc
     4081 aactggctgg atgtcaccgt aggcgctgat atgttgctca atcaaaatat tgccgcctat
     4141 gccgcgctaa ctcaggcaga aaataccact aataatagcg actatctgta tacgatgggg
     4201 gttagcgcca gattttaacg taacagtcac aattgaaacc attaaataac aataattgtg
     4261 gcgatagtgg gtgctaactt accaaataat aaatttggtg aataattgtc gcgtcattca
     4321 ttcctgaact aaggcatttc attccgttct gatggcattt catgccgttt ttccccaggc
     4381 ataaagtgca cttcgttatg gttgtcggca gagatttttc ctttttatta ctgcaggaat
     4441 actgccatga caccttcaaa ttatcagcgt acccgctggc tgacactcat cggtactatc
     4501 attacccagt ttgcgctggg gtcggtttat acctggagcc tgtttaatgg cgcgctttcc
     4561 gccaagctgg atgcgccggt aagccaggtc gctttctctt tcggcttgtt aagtctgggg
     4621 ctggcaattt cgtcttctgt tgcgggcaaa ttacaggaac gtttttgcgt taaacgcgtc
     4681 accatggctt ccggcatttt gctgggatta ggcttcttcc tgaccgcgca ttccaacaac
     4741 ctgatgatgc tgtggttaag cgccggtgtg ctggtcggtc tggcggatgg cgcgggttac
     4801 ctgctgacgc tctctaactg cgtgaagtgg ttcccggagc gtaaaggtct gatctccgcg
     4861 ttcgctatcg gttcttatgg tctgggtagc ctgggtttca aatttatcga cacgcagctg
     4921 ctcgaaacgg tgggtctgga aaaaacattt gtgatttggg gagcgattgc gctggtgatg
     4981 attgttttcg gcgcaacgtt aatgaaagac gcaccaaaac aggaagtgaa aaccagcaat
     5041 ggtgtggtgg agaaagatta cacgctggca gagtcgatgc gtaaaccgca gtactggatg
     5101 ttagcggtaa tgttcctgac tgcctgcatg agcggcctgt acgtgattgg ggtagcgaaa
     5161 gatatcgccc aaagtctggc acaccttgat gtggtttccg cagccaatgc agtaacggtt
     5221 atttccatcg ctaacctttc aggtcgtctg gtgctgggca ttctgtctga caaaattgcc
     5281 cgtatccgtg ttatcaccat tggtcaggtg atttcgctgg tgggtatggc ggccctgctg
     5341 tttgcaccat taaatgcagt gacgttcttt gcagcgattg cctgcgtggc atttaacttt
     5401 ggcggcacta ttaccgtctt tccatcactg gtcagtgagt tcttcggcct caataacctg
     5461 gcgaaaaact acggtgtgat ttacctcggt ttcggtatcg gtagcatttt tggttcgatt
     5521 atcgcctcac tgtttggcgg tttctatgtg accttctacg tgattttcgc cctgctgatt
     5581 ctgtcattgg cgctttctac gacgattcgt cagccagagc agaaaatgtt gcgtgaggcg
     5641 catggctccc tttaatgctc agccgcgagc tactgggctt cagcttccgt gtgcatcaaa
     5701 cgcatcaacg aaatgtataa acaatgactc gtaaaatttg ggccagttaa ctggcccttt
     5761 ttactgcttt ttgtaacgct aaagtctctt ttcaaacttg catttttgta aatttgtgct
     5821 tcatgcacac tctttcccca cactttttcg ctttgctgtg gtctacttat tcgcgcgtgt
     5881 agattttact tatctgacta cctccgcact tttcccctgc cgggcctgaa aagccgttaa
     5941 gcagggtgtt atcacctgtt tgtccagggt ttgtttgcat gagatacatc aaatcgatta
     6001 cacagcagaa gctgagcttt ttgcttgcaa tctatattgg cctttttatg aatggcgcgg
     6061 ttttttatcg ccgcttcggc agctatgcgc acgattttac cgtctggaaa ggcgtttctg
     6121 ctgttgttga actggccgcc accgtactgg tgaccttctt tttactacgt cttctatcgc
     6181 tgtttggccg ccgcagctgg cgtattctgg cgtcgctggt ggtgctcttt tccgcaggtg
     6241 ccagctatta catgaccttc cttaatgtgg tcattggtta tggcatcatc gcttccgtca
     6301 tgaccaccga tatcgacctg tcaaaagaag ttgttggtct gaactttatt ctctggttaa
     6361 tcgccgttag tgcattgcct cttatcctta tctggaataa ccgctgtcgc tacaccttgc
     6421 tccgacaact gcgaacccca gggcagcgta ttcgcagcct ggcggtcgtc gtactggcgg
     6481 gtattatggt ttgggcaccg attcgtttgc tggatatcca gcagaagaaa gtggagaggg
     6541 cgaccggcgt tgatttgccg agttatggcg gtgtcgtagc gaactcttat ctgccatcaa
     6601 actggctttc tgcgttgggg ctgtatgcct gggcgcgggt cgatgaatct tccgataata
     6661 attcattgct taatccggcg aagaagttta cataccaggc tccacaaaac gttgatgaga
     6721 cttatgtcgt gtttatcatc ggtgaaacca cgcgttggga ccatatgggt attttcggct
     6781 atgagcgtaa taccacgccg aaactggccc aggagaaaaa tctggcggcg ttccgtggtt
     6841 actcctgtga taccgcaacc aaactctcac tgcgttgcat gtttgtacgt caggggggcg
     6901 cggaagataa tccgcagcgc acattaaaag aacagaacat tttcgcggtt ctgaagcagt
     6961 taggattcag ttctgacctc tacgctatgc agagcgaaat gtggttctac agcaacacga
     7021 tggcggacaa cattgcttat cgtgagcaga ttggtgcgga gccacgtaat cgtggcaagc
     7081 cggtagatga tatgttgctg gtagacgaaa tgcagcaatc gctagggcgc aacccggatg
     7141 gtaagcatct gatcattctg cataccaaag gttcgcattt taactacacc cagcgttatc
     7201 cgcgtagctt cgcgcagtgg aagccggaat gtattggtgt tgatagcggc tgtaccaaag
     7261 cgcagatgat caactcctat gacaactcgg tgacctatgt ggatcacttt atctccagcg
     7321 tgattgatca ggttcgcgat aagaaagcga ttgtgttcta cgcagctgac cacggtgagt
     7381 caattaatga acgcgagcac ctgcacggca cgccgcgtga actggcaccg ccggagcagt
     7441 tccgcgtacc gatgatggtc tggatgtcag ataaatatct ggaaaatccg gccaatgcgc
     7501 aggcgtttgc gcagctgaaa aaagaagccg acatgaaagt gccacgccgt cacgtagagc
     7561 tgtacgacac catcatgggt tgtcttggct atacttcacc ggatggtgga attaacgaaa
     7621 acaacaactg gtgtcacatc ccgcagacaa aagaggcagc ggctaactaa gaggcctgct
     7681 gactttctcg ccgatcaaaa ggcattttgc tattaaggga ttgacgaggg cgtatctgcg
     7741 cagtaagatg cgccccgcat tcggtgattg gcgcagcttg gtagcgcact tcgttcggga
     7801 cgaaggggtc ggaggttcga atcctctatc accgaccaaa ttcgaaaagc ctgctcagcg
     7861 agcaggcttt tttgcatttg aagcccatga ggatgagaac ctccgggggc ggggaggttc
     7921 gactcgagcg aagcgagaga acgttgcgcc agcaacggcc cgcagggcga gccacgaagt
     7981 ggcgagtaat cctctatcac cgaccaaatt cgaaaagcct gctcaacgag caggcttttt
     8041 tgcatttgta gctcatgagg atgagaacct ccgggggcag ggaggttcga ctcgacgtag
     8101 gcctgataag acgcgccagc gtcgcatcag gcaacggcac cgcatttatt gtcggatgcg
     8161 gcgcgaccgc cttattcgac caacacacca cacattttcc tgccaacttt gcgccccata
     8221 tctcactatc cattaacgga tttgtgacag aaaccattgt ctttttttat atttgcttaa
     8281 atcttttttt gcgttactta tggctggcgc tggcattttc cgctgtgcgt tttagcgttc
     8341 atggtattaa tcgctcagat atccaccttt tcctcagatt tttttcatta aatgcataaa
     8401 acttaatcac caaatgttgc taaatattaa gctgattgtt ctggttatgt tggagtagca
     8461 caaatttctc tgctgcaaat agctgtttga agttttttta atctttgttt gtggattctc
     8521 accgttggtg taaatcccgg ttggctgtat tgacgttttc acattctgtt gacagattgt
     8581 aggtcacgag gggcatttta tggaggatcc gcactgttac actgatgtta attagtacgg
     8641 catccccacc tcataacgtt gacccgaccg ggcaaaaaac aaaaaaggtc aggcagcgac
     8701 aacccactgc aaagggttaa aacaacaaac atcacaattg gagcagaata atgcgtattt
     8761 ccttgaaaaa gtcagggatg ctgaagcttg gtctcagcct ggtggctatg accgtcgcag
     8821 caagtgttca ggctaaaact ctggtttatt gctcagaagg atctccggaa gggtttaacc
     8881 cgcagctgtt tacctccggc accacctatg acgcctcttc cgtaccgctt tataaccgcc
     8941 tggttgaatt taaaatcggc accactgaag tgatcccggg cctcgctgaa aagtgggaag
     9001 tcagcgaaga cggtaaaacc tataccttcc atctgcgtaa aggtgtgaag tggcacgaca
     9061 ataaagaatt caaaccgacg cgtgaactga acgccgatga cgtggtgttc tcgttcgacc
     9121 gtcagaaaaa cgcgcaaaac ccgtaccata aagtctctgg cggcagctac gaatacttcg
     9181 aaggcatggg cttgccggag ctgatcagtg aagtgaaaaa ggtggacgac aacacagttc
     9241 agttcgtact gactcgcccg gaagcgccgt tcctcgctga tctggcaatg gacttcgcct
     9301 ctattctgtc aaaagaatat gctgatgcga tgatgaaagc cggtacaccg gaaaaactgg
     9361 acctcaaccc aatcggaacc ggtccgttcc agttacagca gtaccaaaaa gattcccgta
     9421 tccgctacaa agcgtttgat ggctactggg gcaccaaacc gcagatcgat acgctggttt
     9481 tctctattac ccctgatgct tccgtgcgtt acgcgaaatt gcagaagaat gaatgccagg
     9541 tgatgccgta cccgaacccg gcagacatcg ctcgcatgaa gcaggataaa tccatcaacc
     9601 tgatggaaat gccggggctg aacgtcggct acctctccta taacgtgcag aaaaaaccgc
     9661 tggatgacgt gaaagttcgc caggcactga cctacgcggt gaacaaagac gcgatcatca
     9721 aagcggttta tcagggcgcg ggtgtatcag cgaaaaacct gatcccgcca accatgtggg
     9781 gctataacga cgacgttcag gattacacct acgatcctga aaaagcgaaa gccttgctga
     9841 aagaagcggg tctggaaaaa ggtttctcca tcgacctgtg ggcgatgccg gtacaacgtc
     9901 cgtataaccc gaacgctcgc cgcatggcgg agatgattca ggcagactgg gcgaaagtgg
     9961 gcgtgcaggc caaaatcgtc acctacgaat ggggtgagta cctcaagcgt gcgaaagatg
    10021 gcgaacatca gacggtaatg atgggctgga ctggcgataa cggggacccg gataacttct
    10081 tcgccaccct gttcagctgc gccgcctctg aacaaggttc taactactca aaatggtgct
    10141 acaaaccgtt tgaagatctg attcaaccgg cgcgtgctac cgacgaccat aacaaacgcg
    10201 ttgaactgta caagcaagcg caggtggtga tgcacgatca gactccggca ctgatcatcg
    10261 ctcactccac cgtgtttgaa ccggtacgca aagaagtcaa aggctatgtg gttgatccat
    10321 taggcaaaca tcacttcgaa aacgtctcta tcgaataatt aaaagccata caagactgat
    10381 ggcaaaggca aaactgcctg atgtgctccg cttatcaggc ctacgaaaat tctgcaatgt
    10441 attgaatttg cacgattttg taggccggat aaggcgttaa cgccgcatcc ggcataaaca
    10501 aagcgcactt tgtcaacaat ctgtataccc ggtggcgatg tgcctctgcg tgcagcgcca
    10561 tccggcagca atacttcatt ccctgtccga taagggcggg aattgatttg tgagcaatac
    10621 agacacgcag ttccaggctg cgggtcacta tagagaatcc gggttatgtt gcagtttatt
    10681 ctccgacgtt tgggactcgt catccccacg tttatcggta ttacccttct cacatttgcc
    10741 tttgtccaca tgatcccggg cgatccggtg atgatcatgg caggcgaacg cgggatctcc
    10801 cctgagcgtc acgcgcagct gctggctgaa ctcggcttag ataaaccgat gtggcagcag
    10861 tatctccatt acatttgggg cgtaatgcac ggcgatctag gcatttcaat gaaaagccga
    10921 attccagtat gggaagagtt cgtgccgcgc tttcaggcca cgctggaact tggcgtctgc
    10981 gcgatgattt ttgctacggc agtcggtatt ccggttggtg tgctggctgc ggttaaacgc
    11041 ggttccattt tcgatcacac tgcagttggc ctggcgctga ccggctactc gatgccgatc
    11101 ttctggtggg gcatgatgct gatcatgctg gtttcggtgc actggaacct gacgcccgtc
    11161 tccggtcgcg tgagcgatat ggtgttcctc gatgactcca atccgttaac cggttttatg
    11221 ctgatcgaca ccgccatctg gggtgaagat ggcaacttta tcgacgccgt tgcccatatg
    11281 atcttaccgg ctattgtgct gggtactatc ccgctggcgg ttatcgttcg tatgacgcgc
    11341 tcttcgatgc tggaagtgct gggtgaagat tacatccgca ccgcgcgcgc caaagggttg
    11401 acccgcatgc gggtgattat cgtccatgcg ctgcgtaacg cgatgctgcc ggtagtgacc
    11461 gttatcggcc tgcaggtggg aacattgctg gcgggggcga ttctgaccga aaccatcttc
    11521 tcgtggcccg gtctgggacg ctggttgatt gacgcactgc aacgccgcga ctatccggta
    11581 gtgcagggcg gcgtattgct ggtggcgacg atgattatcc tcgtcaacct gctggtcgat
    11641 ctgctgtacg gcgtggtgaa cccgcgtatt cgtcataaga agtaagggga catcatgtca
    11701 caggttactg aaaataaagt gattagcgca ccggtgccga tgaccccgtt acaggagttc
    11761 tggcactatt ttaaacgcaa caaaggcgcg gttgtcgggc tggtttacat cgtcatcgtg
    11821 ctgtttatca cgatctttgc caactggatt gcgccttata acccggcgga tcagttccgc
    11881 gatgcgctgc tcgccccgcc agcctggcag gaaggcggca gcatggcgca cttgctgggc
    11941 accgatgacg taggccgtga tgtgctgtcg cggctgatgt acggcgcgcg cttgtcgctg
    12001 ctggttggct gtctggtggt ggtgttatcg ctgattatgg gcgttattct cggcctgatc
    12061 gccggttact tcggcggcct ggtcgataac atcatcatgc gcgtggtcga tatcatgctg
    12121 gcgctgccaa gtctgctgtt ggcactggtg ctggtggcga ttttcggccc gtcgattggt
    12181 aacgccgcgc tggcactgac cttcgttgcc ttgccgcact atgtgcgctt aacccgcgcc
    12241 gccgtgctgg tggaggtgaa tcgcgattac gttaccgcgt ctcgcgtggc aggtgccggg
    12301 gcgatgcgtc agatgtttat taacatcttc ccgaactgcc tggcgccgct gattgttcag
    12361 gcgtcgctcg gtttctctaa cgccattctc gatatggccg cccttggctt cctcggcatg
    12421 ggagcgcagc cgccaacacc agagtggggc accatgctct ccgacgtgtt gcagttcgcg
    12481 caaagcgcct ggtgggtcgt gaccttcccg ggtctggcga tcctgctgac ggtgctggca
    12541 tttaacctga tgggtgacgg tctgcgtgac gcgctcgatc ccaaactgaa gcagtaagag
    12601 gttcgagatg gcgttattaa atgtagataa attatcggtg catttcggcg acgaaagcgc
    12661 accgttccgc gccgtagacc gcatcagcta cagcgtaaaa cagggcgaag tggtcgggat
    12721 tgtgggtgag tctggctccg gtaagtcggt cagttcactg gcgattatgg ggctgattga
    12781 ttatccgggc cgcgtaatgg cggaaaagct ggtgtttaac ggtcaggatt tgcagcgtat
    12841 atcagaaaaa gagcgccgca acctggtggg tgccgaagtg gcgatgatct tccaggaccc
    12901 gatgaccagc cttaacccgt gctacaccgt gggtttccag atcatggaag cgattaaggt
    12961 gcatcagggc ggtaataaga gcactcgccg tcagcgggcg attgacctgc tgaatcaggt
    13021 cggtattccc gatccggcat cgcgtctgga tgtttacccg catcagcttt ccggcggcat
    13081 gagccagcgc gtgatgatcg ccatggcgat tgcctgtcgg ccaaaactgc tgattgccga
    13141 tgagccgacc actgcgctgg acgtgaccat tcaggcgcaa atcatcgaac tgttgctgga
    13201 gctacagcag aaagagaaca tggcgctggt gttaattacc catgatctgg cgctggtggc
    13261 ggaagctgcg cataaaatca ttgtgatgta tgcaggccag gtggtggaaa cgggcgatgc
    13321 gcacgccatc ttccatgcgc cgcgtcaccc gtatactcag gcattgctgc gtgcgctgcc
    13381 agaatttgct caggacaaag aacgtctggc gtcgttgcca ggtgtcgttc ccggcaagta
    13441 cgaccgcccg aacggctgcc tgcttaaccc gcgctgcccc tatgccactg acagatgtcg
    13501 cgctgaagaa ccggcgctga atatgctcgc tgacgggcgc cagtccaaat gccattaccc
    13561 acttgatgat gccgggaggc cgacactatg agtacgcaag aggccaccct gcaacaaccg
    13621 ctgttgcagg ctatcgacct gaaaaaacat tatccggtga agaaaggcat gttcgcgccg
    13681 gaacgtctgg ttaaagcgct ggatggcgtt tcgtttaacc ttgaacgtgg caaaacgctg
    13741 gcagtagtgg gcgaatctgg ctgcggtaaa tcgaccctcg gtcggttgct gacgatgatt
    13801 gaaacgccca ccggtggcga gctgtattac caggggcagg atctgcttaa gcacgatccg
    13861 caggcgcaga agctgcgtcg gcagaaaatc cagatcgtct tccagaaccc ttacggttcg
    13921 ctgaatccgc gtaaaaaagt cgggcaaatt cttgaagagc cgctgctgat caacaccagc
    13981 ttaagcaaag aacagcgtcg ggaaaaagcc ctgtcgatga tggcgaaagt cggcctgaaa
    14041 accgagcact atgaccgcta tccgcatatg ttctccggcg gtcagcgtca gcgtatcgcc
    14101 atcgcccgtg gtctgatgct cgacccggat gtggtgattg ccgatgaacc ggtttccgcg
    14161 ctggatgttt cagtgcgcgc gcaggtgctg aatctgatga tggatttgca gcaggagttg
    14221 gggctgtctt atgtctttat ctcccacgac ctgtcggtgg tggagcacat tgctgatgaa
    14281 gtgatggtga tgtacctggg ccgctgcgtg gagaagggaa cgaaagacca aatcttcaat
    14341 aacccgcgcc atccgtacac tcaggcgcta ctttccgcga cgccgcgcct gaacccggac
    14401 gatcgccgcg agcgcatcaa gctcagcggt gaactaccaa gcccactgaa tccaccgccg
    14461 ggttgcgcct tcaacgcccg ctgtcgtcgg cgcttcggcc cctgcaccca gttgcagccg
    14521 cagctaaaag actacggcgg tcaactggta gcttgttttg ctgttgatca ggatgaaaat
    14581 ccgcagcgtt aactcacaaa accggggcca tgcgctccgg tttactcaat cagcttcaac
    14641 agtggcgaca agcatagcaa aatgccgtac agcagaatca gccaggcttt aaatccgcga
    14701 agtttttcca gttgtgcgac tttatagatc aggaaaaacg gaatgaggca cgacacaata
    14761 ccatataacg ggcttcccaa ctgaaagaac accagcaccg atacacgaaa cgaaacccaa
    14821 atcgtcaacg taatgacgat aaaagcgcag atcgccagag tcagcacgcg tgagttaatt
    14881 ttcttggtat caataattcg gcttaacaga ttgagaataa tgcctttaat ggcctcgtgg
    14941 aaaccgagat aaatgccaaa gaatgcggtc agtacggcaa agatattaag caccgtagag
    15001 gtgatatgaa tgatatgccc agggatcacc tgcgcggcca gcgccagtgc tgagatattt
    15061 tgttcaaagg cagaaacggc ttcttcgtgg ctaattgaga aggtaaacga aaaggcaaaa
    15121 aacaggatca ccgcgatgag cgtgatataa ctaattcggt gggtacgcag cgcgagccgg
    15181 gttgccagta ctttatccgc ttcccgttta cgataggcaa tattcattgg gttaagtacc
    15241 tgaataaata ctgcagaaaa gaagcaaaat ggaatggtaa gcaagacatc gcggaaaaag
    15301 acggaggctt gcgggaaggc ggttatattg gcgaaattcc agtgcgggat catcgcaaaa
    15361 ccgaacacga caataatccc tactttgacc accaccattg gcccggaaat cttaaacagt
    15421 aatcgttcac caccagacgc aatcgctacc agtacggcga aaatagcgac tttataaagt
    15481 agagattgtg aaagatcggc atcggttaaa ccgaaggttt tcaggtacga ggcgctgtcg
    15541 aaaaccacgg agagagagta gataaaaatc ccgtggataa tcatcaaaaa gtagataacc
    15601 ccgaggaaaa ttccccagtt cttccccagg taatgactga taatatcggt gtagtcatta
    15661 caggaatcgc tttcagaaag ggtttttaaa taaatgtcct gcactaccca ggtggcagga
    15721 taagcaatga tcgctgcggt aataaatacc caaattccct tcaagccaat ttgtactggc
    15781 atcagcacgg ttccggcacc aatcgccatg cctatgcata ataaaaccca gccgaagtcg
    15841 tagcgtgtaa acggcaattt ctgattgtgt ttcgatagtg tgttgtgctg cataaagtcc
    15901 tgtccagaaa gaagtcggcc tcgcaaaaat gaataaaccc agtttctctt gcgcaacagg
    15961 tggagaagtc gaatacgcag tatgtaaaaa atgaggcaac agattcactg gggaggtcgc
    16021 gaggaggcgc aattctacaa aattgcgcat ttcactacca gtctggagtc agcccggata
    16081 tgaactgcaa ctattgcaaa taaagaaatt aagctggaag gcatcgcgca atgaggaaga
    16141 ttgccgggtg ctgacagtaa aaagcaatcg taaaatcagc ggcaagccg
//
LOCUS       DAJTZL010000089        16070 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.089, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000089 DAJTZL010000000
VERSION     DAJTZL010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16070
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.089"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(1..563)
                     /locus_tag="OO544_002473"
                     /pseudo
     CDS             complement(1..563)
                     /locus_tag="OO544_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            complement(797..2217)
                     /gene="espX1"
                     /locus_tag="OO544_002474"
                     /pseudo
     CDS             complement(797..2217)
                     /gene="espX1"
                     /locus_tag="OO544_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308052.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspX1"
     gene            complement(2702..2965)
                     /gene="rpsT"
                     /locus_tag="OO544_002475"
     CDS             complement(2702..2965)
                     /gene="rpsT"
                     /locus_tag="OO544_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HCR6768047.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
     gene            3068..3286
                     /locus_tag="OO544_002476"
     CDS             3068..3286
                     /locus_tag="OO544_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HCR6768048.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGHFLPVICAMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            3294..4235
                     /gene="ribF"
                     /locus_tag="OO544_002477"
     CDS             3294..4235
                     /gene="ribF"
                     /locus_tag="OO544_002477"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HCR6768049.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKASMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            4278..7094
                     /gene="ileS"
                     /locus_tag="OO544_002478"
     CDS             4278..7094
                     /gene="ileS"
                     /locus_tag="OO544_002478"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HCR6768050.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFAVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELSAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            7094..7588
                     /gene="lspA"
                     /locus_tag="OO544_002479"
     CDS             7094..7588
                     /gene="lspA"
                     /locus_tag="OO544_002479"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HCR6768051.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSR
                     AKKQ"
     gene            7713..8162
                     /gene="fkpB"
                     /locus_tag="OO544_002480"
     CDS             7713..8162
                     /gene="fkpB"
                     /locus_tag="OO544_002480"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR6768052.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            8164..9114
                     /gene="ispH"
                     /locus_tag="OO544_002481"
                     /gene_synonym="lytB"
     CDS             8164..9114
                     /gene="ispH"
                     /locus_tag="OO544_002481"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HCR6768053.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKRAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            9180..10094
                     /gene="rihC"
                     /locus_tag="OO544_002482"
     CDS             9180..10094
                     /gene="rihC"
                     /locus_tag="OO544_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HCR6768054.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPIGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSSGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVKVALDLDVKGFQQWVAEVLALAS"
     gene            10261..11082
                     /gene="dapB"
                     /locus_tag="OO544_002483"
     CDS             10261..11082
                     /gene="dapB"
                     /locus_tag="OO544_002483"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HCR6768055.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAVKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKENGLFDMRDVLNLNNL"
     gene            11538..12686
                     /gene="carA"
                     /locus_tag="OO544_002484"
     CDS             11538..12686
                     /gene="carA"
                     /locus_tag="OO544_002484"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HCR6768056.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
     gene            12704..15925
                     /gene="carB"
                     /locus_tag="OO544_002485"
     CDS             12704..15925
                     /gene="carB"
                     /locus_tag="OO544_002485"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HCR6768057.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     ERLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
BASE COUNT         3921 a         3781 c         4254 g         4114 t
ORIGIN      
        1 tccccccagt agaaaggaat ggagataaac agattaaaac gctcctcttc tttattattt
       61 tcatcataag attggctggc agagaaagta taactaatgt gttgccagtt attggagtaa
      121 ctgaattggt aatctttagc atttccactt cgcccccagt aattccgcca aagcgcactt
      181 aatgatacat ttcccagatt attggataaa ggttgcataa tattggcaga aaggctattt
      241 tttctgccaa tatcataaaa atcatcataa tcactctgat gatagtgttt atcattttcg
      301 taaagatggt cgctgaatgt cctgtaatcc tgcgaagcat aacgccaggc ggccacgcta
      361 aatcgagtgg ctgtttgtac tagatattta ttataggcga cctgatagct cgtaccttcg
      421 tgtctggtat cattatttaa cttactactc gatcgggcag cgtaaaaaga gatggcaccc
      481 agcggtgtat tccaaccatt tcccagagtt atagcattat agttatcgga taaaatcgta
      541 cccgattatg tgatcaggtt gttattattt agattaatgt ttaaattttt taatttcttg
      601 atgtgaagtt gatgaaagtt tgaaaattta atattcttaa ttgttccctt ctttataaga
      661 agagcaaggt gccatcatta gagtgacaga gtaggatgtt tcagagcatg cttcagattg
      721 ttttttattt ctctgcagtg tattaataat catcacagat aatttcattc taaaattacc
      781 tctttctttg agtgttttaa tcctgatgaa ctatattttt tataaggagc atgcactcgt
      841 tgatgctctg aaggtatttt ctcatttttg atgatgtttt gtcgtaatcg tgcataaact
      901 tattaagttc tttatacaca tcattattta gctttacatt atcgcgaact aaagtttgta
      961 ccaatggtat caaaatttcg aaacgggacg gtttgtcatc aatacttgcc agggttttga
     1021 atactcctgt gtatctgtaa cctaagtgct tattgacaag ttgagtggtc catcggccag
     1081 gcataatatc ttcacagcgc aaatttttta tcatggtatt agtgaacgat gttccaaata
     1141 tgttgctttt tttaatgaag aggttgttta tttctccatt tgaaaagatg gtgtttgata
     1201 agtcgctttc aaaacatgat atattggtta atacacaaca aaaataatac ccatatgaaa
     1261 ggttgctctt ttctattgtt atatttgata gcaacatttt atgtatatta atattaagag
     1321 cagtcaaata ctggatcttt tgatcgttaa gttcatacat ctccagtgat ttattatagg
     1381 gtaataaaag tataaattcc tttatattat ctccgcagta agaaaagacg acattaaatt
     1441 tattagtggc catgttttca gttgagcagt aagctgattt ttctattaac cgactgggca
     1501 cgcttttaaa taaagataat tttccacata gttttgaata tgccgaattg ctagttttaa
     1561 agttctgcca cattattccc tggttatcat ctatcgatga ggaggggatt ttattgcatt
     1621 gctcattgta tagctcaggg aaaatatttt ttttaataag ctttttgatt tcctggttaa
     1681 gatgactttt tttcttgtct attgcactgt attttgcgtt ttgtgacgtt tcctgatgat
     1741 cataaaatga ctggagattt ctgcaggcct cataaaatat ttcaggagta tatagctgtg
     1801 ggtatatttc ttggttgaag tttgccttta aaaaattaac gaatttttca tggcattctt
     1861 ggtatatctt aatctcttca gaaagacatt cgcttgttct gtcatcaaaa acatccaatc
     1921 taagtaatgt gatacctttg tgtttaaaaa ataaaattga actgtatgtt ctatgagttt
     1981 acatctataa aaatatgcat ctctcagatg agtatattga aaaaaatgat gaaggactgg
     2041 agatggttta ggcaagttat ggcttctaaa aaagtcatca aacaaaatta agaactctgt
     2101 tttatactcg caaaatactt ttactaactc attttgtctt gttttattta ttattacatc
     2161 atctactata tttgatttat aaagacaatg tggcgaagtt gagataccat ttgtcattat
     2221 cagtccttga agttgatgaa tttttattaa tttaatttat gagtgaatgt gactactatc
     2281 aaaaatagct cattaatttt atgaagtaga atataaatag gtatttaacc tgtgttgatt
     2341 gttgttaatt atttacaaga tctaataagg agatatttat gaatggttaa agtgtatagg
     2401 aatttaattc aaaaatagag tggtcttaag tttaatacct caatgtgtat cacaatatcc
     2461 acattctttg taggggagtc tggagattga gtagatattc ttgttcagaa tgtatcagcc
     2521 gatggttcta cgattcttaa gccacgaaga gttcagaaag tgtaacggca tgtctgcaaa
     2581 agccagcatc ttttgactat ctggcaaccg gtagtgtgtt ctctcacgca tcataaaagc
     2641 agcaggcata aaaaaaaccc gcttgcgcgg gctttttcac aaagcttcag caaattggcg
     2701 attaagccag tttgttgatc tgtgcagtca ggttagcctt atgacgtgca gctttgtttt
     2761 tgtggatcag acctttagca gcctgacggt ccacgatcgg ttgcatttcg ttaaatgctt
     2821 tctgtgcagc agctttgtcg ccagcttcga tagctgcgta tactttcttg atgaaagtac
     2881 gcatcataga gcgacggctt gcgttgtgct tacgagcctt ttcagactga atggcgcgct
     2941 tcttagctga tttgatatta gccaaggtcc aactcccaaa tgtgttctat atggacaatt
     3001 caaaggccga ggaatatgcc cttttagcct tcttttgtca atggatttgt gcaaataagc
     3061 gccgttaatg tgccggcact cgttacgtag tgatggcgca ggattctacc agcttgcggg
     3121 gtgtgaatac agcttttccg cgataaaaat tgcagcaggc ggtcactttc ttcccgtgat
     3181 ttgcgccatg gcaatgaaaa gccacttctt tctgatttcg gtactcaatc gccggttaac
     3241 cttgaccgct gtacaaggta tactcggacg attttcactg ttttgagcca gacatgaagc
     3301 tgatacgcgg catacataat ctcagccagg ccccgcaaga agggtgtgtg ctgactattg
     3361 gtaatttcga cggcgtgcat cgcggtcatc gcgcgctgtt acagggcttg caggaagaag
     3421 ggcgcaagcg caacttaccg gtgatggtga tgctttttga acctcaacca ctggaactgt
     3481 ttgctaccga taaagccccg gcaagactga cccggctgcg ggaaaaactg cgttaccttg
     3541 cagagtgtgg cgttgattac gtgctgtgcg tgcgtttcga caggcgtttc gcggcgttaa
     3601 ccgcgcaaaa tttcatcagc gatcttctgg tgaagcattt gcgcgtaaaa tttcttgccg
     3661 taggggatga tttccgcttt ggcgctggtc gtgaaggcga tttcttgtta ttacagaaag
     3721 ctagcatgga atacggcttc gatatcacta gtacgcaaac tttttgcgaa ggtggcgtgc
     3781 gtattagcag caccgctgtg cgtcaggcgc tggcggatga caatctggct ctggcagaaa
     3841 gtttactggg gcacccgttt gccatctccg ggcgtgtagt ccacggtgat gaattagggc
     3901 gcactatagg tttcccgacg gcgaatgtac cgctacgccg tcaggtttcc ccggtgaaag
     3961 gggtttatgc ggtagaagtg ctgggcctcg gtgaaaagcc gttacccggc gtggcaaaca
     4021 tcggaacacg cccaacggtt gccggtattc gccagcagct ggaagtgcat ttgttagatg
     4081 ttgcaatgga cctttacggt cgccatatac aagtagtgct gcgtaaaaaa atacgcaatg
     4141 agcagcgatt tgcgtcgctg gacgaactga aagcgcagat tgcgcgtgat gaattaaccg
     4201 cccgcgaatt ttttgggcta acaaaaccgg cttaagcctg ttatgtaatc aaaccgaaac
     4261 acggaaccga gaatctgatg agtgactata aatcaaccct gaatttgccg gaaacagggt
     4321 tcccgatgcg tggcgatctc gccaagcgcg aaccgggaat gctggcgcgt tggactgatg
     4381 atgatctgta cggcatcatc cgtgcggcta aaaaaggcaa aaaaaccttc attctgcatg
     4441 atggccctcc ttatgcgaat ggcagcattc atattggtca ctcggttaac aagattctga
     4501 aagacattat cgtgaagtcc aaagggcttt ccggttatga ctcgccgtat gtgcctggct
     4561 gggactgcca cggtctgccg atcgagctga aagtagagca agaatacggt aagccgggtg
     4621 agaaattcac cgccgccgag ttccgcgcca agtgccgcga atacgcggcg acccaggttg
     4681 acggtcaacg caaagacttt atccgtctgg gcgtgctggg cgactggtcg cacccgtacc
     4741 tgaccatgga cttcaaaact gaagccaaca tcatccgcgc gctgggcaaa atcatcggca
     4801 acggtcacct gcacaaaggc gcgaagccag ttcactggtg cgttgactgc cgttctgcgc
     4861 tggcggaagc ggaagttgag tattacgaca aaacttctcc gtccatcgac gttgcttttc
     4921 aggcggtcga tcaggatgca ctgaaagcaa aatttgccgt aagcaacgtt aacggcccaa
     4981 tctcgctggt gatctggacc actacgccgt ggactctgcc tgcgaaccgc gcaatctcta
     5041 ttgcacctga tttcgactat gcgctggtgc agatcgacgg tcaggccgtg attctggcga
     5101 aagatctggt tgaaagcgta atgcagcgta tcggcgtgac cgattacacc attctcggca
     5161 cggtaaaagg tgcggagctt gagttgctgc gctttaccca tccgtttatg ggcttcgacg
     5221 ttccggcaat cctcggcgat cacgttaccc tagatgccgg taccggtgcc gttcacaccg
     5281 cgcctggcca cggcccggac gactatgtga tcggtcagaa atacggcctg gaaaccgcta
     5341 acccggttgg cccggacggc acttatctgc cgggcactta tccgacgctg gatggcgtga
     5401 acgtcttcaa agcgaacgac atcgtcgttg cgctgctgca ggaaaaaggc gcgctgctgc
     5461 acgttgagaa aatgcagcac agctatccgt gctgctggcg tcacaaaacg ccgatcatct
     5521 tccgcgcgac gccgcagtgg ttcgtcagca tggatcagaa aggtctgcgt gcgcagtcac
     5581 tgaaagagat caaaggcgtg cagtggatcc cggactgggg ccaggcgcgt atcgagtcga
     5641 tggttgctaa ccgtcctgac tggtgtatct cccgtcagcg cacctggggc gtaccgatgt
     5701 cactgttcgt gcacaaagac acggaagagc tgcatccgcg tactctcgaa ctaatggaag
     5761 aagtggcaaa acgcgttgaa gttgacggca tccaggcgtg gtgggatctt gatgcgaaag
     5821 agatcctcgg cgatgaagct gatcagtacg tgaaagtgcc ggacacattg gatgtatggt
     5881 ttgactccgg atctacccac tcttctgttg ttgacgtgcg tccggaattt gccggtcacg
     5941 cagcggacat gtatctggaa ggttctgacc agcaccgtgg ttggttcatg tcttccctaa
     6001 tgatctccac cgcgatgaaa ggcaaagcgc cgtatcgtca ggtactgacc cacggcttta
     6061 ccgtggatgg tcagggtcgc aagatgtcta aatccatcgg caataccgtt tcgccgcagg
     6121 atgtgatgaa caaactgggc gcggatattc tgcgtctgtg ggtggcatca accgactaca
     6181 ccggtgaaat ggccgtttct gacgagatcc tgaaacgtgc tgccgatagc tatcgtcgta
     6241 tccgtaacac cgcgcgcttc ctgctggcaa acctgaacgg ttttgatcca gcaaaagata
     6301 tggtgaaacc ggaagagatg gtggtactgg atcgctgggc cgtaggttgt gcgaaagcgg
     6361 cacaggaaga catcctcaag gcgtacgaag catacgattt ccacgaagtg gtacagcgtc
     6421 tgatgcgctt ctgctccgtt gagatgggtt ccttctacct cgacatcatc aaagaccgtc
     6481 agtacaccgc caaagcggac agtgtggcgc gtcgtagctg ccagactgcg ctgtatcaca
     6541 tcgcagaagc gctggtgcgc tggatggcac caatcctctc cttcaccgct gatgaagtgt
     6601 ggggctacct gccgggcgaa cgtgaaaaat acgtcttcac cggtgagtgg tacgaaggcc
     6661 tgtttggcct ggcagacagt gaagcgatga acgatgcgtt ctgggacgag ctgttgaaag
     6721 tgcgtggcga agtgaacaaa gtcattgagc aagcgcgtgc cgacaagaaa gtgggtggct
     6781 cgctggaagc ggcagtaacc ttgtatgcag aaccggaact gtcggcgaaa ctgaccgcgc
     6841 tgggcgatga attacgattt gtcctgttga cctccggcgc taccgttgca gactataacg
     6901 acgcacctgc tgatgctcag cagagcgaag tactcaaagg gctgaaagtc gcgttgagta
     6961 aagccgaagg tgagaagtgc ccacgctgct ggcactacac ccaggatgtc ggcaaggtgg
     7021 cggaacacgc agaaatctgc ggccgctgtg tcagcaacgt cgccggtgac ggtgaaaaac
     7081 gtaagtttgc ctgatgagtc aatcgatctg ttcaacaggg ctacgctggc tgtggctggt
     7141 ggtagtcgtg ctgattatcg atctgggcag caaatacctg atcctccaga actttgctct
     7201 gggggatacg gtcccgctgt tcccgtcgct taatctgcat tatgcgcgta actatggcgc
     7261 ggcgtttagt ttccttgccg atagcggcgg ctggcagcgt tggttctttg ccggtattgc
     7321 gattggtatt agcgtgatcc tggcagtgat gatgtatcgc tcgaaggcca cgcagaagct
     7381 aaacaatatc gcttacgcgc tgattattgg cggcgcgctg ggcaacctgt tcgaccgcct
     7441 gtggcacggc ttcgttgtcg atatgatcga cttctacgtc ggcgactggc acttcgccac
     7501 cttcaacctt gccgatactg ccatctgtgt cggtgcggca ctgattgtgc tggaaggttt
     7561 tttgccttct agagcgaaaa aacaataata aaccctgccg gatgcgatgc tgacgcatct
     7621 tatccggcct acagattgct gcgaaatcgt aggccggata aggcgtttac gccgcatccg
     7681 gcaaaaatcc tcaaatataa gagcaaacct gcatgtctga atctgtacag agcaatagcg
     7741 ccgtcctggt gcacttcacg ctaaaactcg acgatggcac caccgccgag tctacccgca
     7801 acaacggtaa accggcgctg ttccgcctgg gtgatgcttc tctttctgaa gggctggagc
     7861 aacacctgtt ggggctgaaa gtgggcgata aaaccacctt ctcgttggag ccagatgcgg
     7921 cgtttggcgt gccgtcaccg gacctgattc agtacttttc ccgccgtgaa tttatggatg
     7981 caggcgagcc agaaattggc gcaatcatgc tttttaccgc aatggatggc agtgagatgc
     8041 ctggcgtgat ccgcgaaatt aacggcgact ccattaccgt tgatttcaac catccgctgg
     8101 ccgggcagac cgttcatttt gatattgaag tgctggaaat cgatccggca ttggaggcgt
     8161 aacatgcaga tcctgttggc caacccgcgt ggtttttgtg ccggggtaga ccgcgctatc
     8221 agcattgttg aaaacgcgct ggccatttac ggcgcaccga tatatgtccg tcacgaagtg
     8281 gtacataacc gctatgtggt cgatagcttg cgtgagcgtg gggctatctt tattgagcag
     8341 attagcgaag taccggacgg cgcgatcctg attttctccg cacacggtgt ttctcaggcg
     8401 gtacgtaacg aagcaaaaag ccgcgatttg acggtgtttg atgccacctg tccgctggtg
     8461 accaaagtgc atatggaagt cgcccgcgcc agccgtcgtg gcgaagagtc gattctcatc
     8521 ggccacgccg ggcatccgga agtggaaggt acaatgggcc agtacagtaa cccggaaggg
     8581 ggaatgtatc tggtcgaatc gccggacgat gtgtggaaac tgacggtcaa aaacgaagag
     8641 aagctctcct ttatgaccca gaccacgctg tcggtggatg acacgtctga tgtgatcgac
     8701 gcgctgcgta aacgcttccc gaaaattgtc ggtccgcgca aagatgacat ctgctacgcc
     8761 acgactaacc gtcaggaagc ggtacgcgcc ctggcagaac aggcggaagt tgtgttggtg
     8821 gtcggttcga aaaactcctc caactccaac cgtctggcgg agctggccca gcgtatgggc
     8881 aaacgcgcgt ttttgattga cgatgcgaaa gatatccagg aagagtgggt gaaagaggtt
     8941 aaatgcgtcg gcgtgactgc gggcgcatcg gctccggata ttctggtgca aaatgtggtg
     9001 gcacgtttgc agcagctggg cggtggtgaa gccattccgc tggaaggccg tgaagaaaac
     9061 attgttttcg aagtgccgaa agagctgcgt gtcgatattc gtgaagtcga ttaagtcatt
     9121 agcagcctaa gttatgcgaa aatgccggtc ttgttaccgg cattttttat ggagaaaaca
     9181 tgcgtttacc tatcttcctc gatactgacc ccggcattga cgatgccgtc gccattgccg
     9241 ccgcgatttt tgcccccgaa ctcgacctgc aactgatgac caccgtcgcg ggtaatgtct
     9301 cggttgagaa aaccacccgc aacgccctgc aactgctgca tttctggaat gcggagattc
     9361 cgctcgccca gggagccgct gtgccgctgg tacgcgcacc gcgtgatgca gcatctgtgc
     9421 acggcgaatc gggaatggct ggctacgact ttgttgagca caaccgaaag ccaatcggga
     9481 taccagcgtt tctggcgatt cgtgatgccc tgatgcgtgc accagagcct gttaccctgg
     9541 tggccattgg cccgttaacc aatattgcgc tgttactttc acaatgcccg gaatgtaaac
     9601 cgtatattcg tcgtctggtg atcatgggcg gttcttctgg tcgcggcaac tgtacaccaa
     9661 atgccgagtt taatatcgct gccgatccag aagcggctgc ctgtgtcttc cgtagcggaa
     9721 tcgaaatcgt catgtgcggt ctggatgtca ccaatcaggc aatattaacc cctgactatc
     9781 tcgctacttt gccggagttg aatcgcaccg gaaaaatgct tcatgcactg tttagccact
     9841 accgtagcgg cagtatgcaa agcggtctgc gaatgcacga tctctgcgcc atcgcctggc
     9901 tggtgcgccc ggacttattc accctcaagc cgtgttttgt cgcggtagaa actcaaggcg
     9961 aatttacctc aggcacgacg gtggttgata tcgacggttg cctgggcaaa ccagccaatg
    10021 taaaggtggc attggatctg gatgtgaaag gcttccagca gtgggtggct gaggtgctgg
    10081 ctctggcgtc gtaacctgtc acatgttatc ggcatgcagt cattcatcga ctcatgcctt
    10141 tcactgatat ccctccctgt ttatcattaa tttctaatta tcagcgtttt tggctggcgg
    10201 cgtagcgatg cgctggttac tctgaaaacg gtctatgcaa attaacaaaa gagaatagct
    10261 atgcatgatg caaacatccg cgttgccatc gcgggagccg gggggcgtat gggccgccag
    10321 ttgattcagg cggcgctggc attagaaggc gtgcagctgg gcgcggcgct ggagcgcgaa
    10381 ggatcttctt tactgggcag cgacgccggt gagctggctg gtgcagggaa aacaggcgtt
    10441 accgtgcaaa gcagcctcga tgcggtaaaa gatgattttg atgtgtttat cgattttacc
    10501 cgtccggaag gtacgctgaa ccatctcgct ttttgtcgcc agcatggcaa agggatggtg
    10561 atcggcacta cggggtttga cgaagccggt aaacaggcga ttcgtgacgc cgccgccgat
    10621 attgcgattg tctttgccgc caattttagc gttggcgtta acgtcatgct taagctgctg
    10681 gagaaagcag ccaaagtgat gggtgactac accgatatcg aaattattga agcacatcat
    10741 agacataaag ttgatgcgcc gtcaggcacc gcactggcaa tgggagaggc gatcgcccac
    10801 gcccttgata aagatctgaa agattgcgcg gtctacagtc gtgaaggcca caccggtgaa
    10861 cgtgtgcctg gcaccattgg ttttgccacc gtgcgtgcag gtgacatcgt tggtgaacat
    10921 accgcgatgt ttgccgatat tggcgagcgt ctggagatca cccataaggc gtccagccgt
    10981 atgacatttg ctaacggcgc ggtaagatcg gctttgtggt tgagtggtaa ggaaaatggt
    11041 ctttttgata tgcgagatgt acttaatctc aataatttgt aaccacaaaa tatttgttat
    11101 ggtgcaaaaa taacacattt aatttattga ttataaaggg ctttaatttt tggccctttt
    11161 atttttagtg ttatgttttt aaatattcta taagtgccaa aaattacatg ttttgtcttc
    11221 tatttttgtt gttttaatgt aaattttgac catttggtcc actttttcct gctcgttttt
    11281 atttcatgca atcttcttgc tgcgcaagcg ttttccagaa caggttatat gatctttttg
    11341 tcgcttaatg cctgtaaaat atgcatgagc cacaaaataa tataaaaaat accgccatta
    11401 agttgacttt tagcgcccat atctccagaa tgccgccgtt tgccagaaat tcgtcggtaa
    11461 gcagatttgc attgatttac gtcatcattg tgaattaata tgcaaataaa gtgagtgaat
    11521 attctctgga gggtgttttg attaagtcag cgctattggt tctggaagac ggaacccagt
    11581 ttcacggtcg ggccataggg gcaacaggtt cggcggttgg ggaagtcgtt ttcaatactt
    11641 caatgaccgg ttatcaagaa atcctcactg atccttccta ttctcgtcaa atcgttactc
    11701 ttacttatcc ccatattggc aatgtcggca ctaatgacgc cgatgaagaa tcttctcagg
    11761 tacatgcaca aggtctggtg attcgcgatc tgccgctgat tgccagcaac ttccgtaata
    11821 ccgaagacct ctcttcttac ctgaagcgcc ataacatcgt ggcgattgcc gatatcgata
    11881 cccgtaagct gacgcgttta ctgcgcgaga aaggcgcaca gaatggctgc attatcgcag
    11941 gcgataaccc ggatgcggcg ttggcgttag aaaaagcccg cgcgttccca ggtctgaatg
    12001 gcatggatct ggcaaaagaa gtgaccaccg cagaagccta tagctggaca caagggagct
    12061 ggacgttgac cggcggcctg ccagaagcga aaaaagaaga cgagctgccg ttccacgtcg
    12121 tggcttatga ttttggtgcc aagcgcaaca tcctgcggat gctggtggat agaggctgtc
    12181 gcctgaccat cgttccggcg caaacttctg cggaagatgt gctgaaaatg aatccagacg
    12241 gcatcttcct ctccaacggt cctggcgacc cggccccgtg cgattacgcc attaccgcca
    12301 tccagaaatt cctcgaaacc gatattccgg tattcggcat ctgcctcggt catcagctgc
    12361 tggcgctggc gagcggtgcg aagactgtca aaatgaaatt tggtcaccac ggcggcaacc
    12421 atccggttaa agatgtggag aaaaacgtgg tgatgatcac cgcccagaac cacggttttg
    12481 cggtggatga agcaacatta cctgcaaacc tgcgtgtcac gcataaatcc ctgttcgacg
    12541 gtacgttaca gggcattcat cgcactgata aaccggcgtt cagcttccag ggtcacccgg
    12601 aagccagccc tgggccacac gacgcagcgc cgttgttcga ccactttatc gagttaattg
    12661 agcagtaccg taaaaccgct aagtaatcag gagtaaaaga gccatgccaa aacgtacaga
    12721 tataaaaagt atcctgattc tgggtgcggg cccgattgtt atcggtcagg cgtgtgagtt
    12781 tgactactct ggcgcgcaag catgtaaagc cctgcgcgaa gagggttacc gcgtcattct
    12841 ggtgaactcc aacccggcga ccatcatgac cgacccggaa atggccgatg cgacctacat
    12901 cgagccgatt cactgggaag tagtacgcaa gattattgaa aaagagcgcc cggacgcggt
    12961 gctgccaacg atgggcggtc agacggcgct gaactgcgcg ctggagctgg agcgtcaggg
    13021 cgtgttggaa gagttcggcg tgactatgat tggtgcgacc gccgatgcga ttgataaagc
    13081 agaagaccgc cgtcgtttcg acgtagcgat gaagaaaatt ggtctggaaa ccgcgcgttc
    13141 cggtatcgca cacacgatgg aagaagcgct ggcggttgcc gctgacgtgg gcttcccgtg
    13201 cattattcgc ccatccttta ccatgggcgg tagcggcggc ggtatcgctt ataaccgcga
    13261 agagtttgaa gaaatttgcg cccgcggtct ggatctctcc ccaaccaaag agctgctgat
    13321 tgatgagtcg ctgatcggct ggaaagagta cgagatggaa gtggtgcgtg ataaaaacga
    13381 caactgcatc atcgtctgct ctatcgaaaa ctttgatgcg atgggcatcc ataccggtga
    13441 ctccatcact gtcgcgccag cccaaacgct gaccgacaaa gaatatcaaa tcatgcgtaa
    13501 cgcctcgatg gcggtgctgc gtgaaatcgg cgttgaaacc ggtggttcca atgtccagtt
    13561 tgcggtgaac ccgaaaaacg gtcgcctgat tgttatcgaa atgaacccac gcgtgtcccg
    13621 ttcttcggcg ctggcgtcga aagcgaccgg tttcccgatt gctaaagtgg cggcgaaact
    13681 ggcggtgggt tacaccctcg acgaactgat gaacgacatc actggcggac gtactccggc
    13741 ctccttcgag ccgtccatcg actatgtggt tactaaaatt cctcgcttca acttcgaaaa
    13801 attcgctggt gctaacgacc gtctgaccac tcagatgaaa tcggttggcg aagtgatggc
    13861 gattggtcgc acgcagcagg aatccctgca aaaagcgctg cgcggcctgg aagtcggtgc
    13921 gactggattc gacccgaaag tgagcctcga tgacccggaa gcgttaacca aaatccgtcg
    13981 cgaactgaaa gatgctggcg cagagcgtat ctggtacatc gccgatgcct tccgtgcggg
    14041 cctgtctgtg gacggcgtgt tcaacctgac caatattgac cgctggttcc tggtacagat
    14101 tgaagaactg gtgcgtctgg aagagaaagt agcggaagtg ggcatcactg gcctgaacgc
    14161 tgaattcctg cgccagctga aacgcaaagg ctttgccgat gcgcgtctgg caaaactggc
    14221 gggcgtgcgc gaagcggaaa tccgcaagct gcgcgaccag tatgacctgc acccggttta
    14281 taagcgcgtg gatacctgtg cggcagagtt cgccaccgac accgcttaca tgtactccac
    14341 ttatgaagaa gagtgcgaag cgaatccgtc taccgaccgt gaaaaaatca tggttctcgg
    14401 cggcggcccg aaccgtatcg gtcagggtat cgaattcgac tactgctgtg tacacgcctc
    14461 gctggcgctg cgcgaagacg gttacgaaac cattatggtt aactgtaacc cggaaaccgt
    14521 ctccaccgac tacgacactt ccgatcgcct ctacttcgag ccggtaactc tggaagatgt
    14581 gctggaaatc gtgcgtattg agaagccgaa aggcgttatc gttcagtacg gcggtcagac
    14641 cccgctgaaa ctggcgcgcg cgctggaagc tgctggcgta ccggttatcg gcaccagccc
    14701 ggatgctatc gaccgtgcag aagaccgtga acgcttccag catgcggttg agcgtctgaa
    14761 actgaaacaa ccggcgaacg ccaccgttac cgctattgaa atggcggtag agaaggcgaa
    14821 agagattggc tacccactgg tggtacgtcc gtcttacgtt ctcggcggtc gggcgatgga
    14881 aatcgtctat gacgaagctg acctgcgtcg ctacttccag acggcggtca gcgtgtctaa
    14941 cgatgcgcca gtgttgctgg atcacttcct tgatgacgcg gtagaagttg acgtggatgc
    15001 catctgcgac ggcgaaatgg tgctgattgg cggcatcatg gagcacatcg agcaggcggg
    15061 cgtgcactcc ggtgactccg catgttctct gccagcctac accttaagtc aggaaattca
    15121 ggatgtgatg cgccagcagg tgcagaaact ggccttcgaa ttgcaggtgc gcggtctgat
    15181 gaacgtgcag tttgcggtga aaaacaacga agtctacctg attgaagtta acccgcgtgc
    15241 ggcgcgtacc gttccgttcg tctccaaagc caccggcgta ccgctggcaa aagtggcggc
    15301 gcgtgtgatg gctggcaaat cgctggctga gcagggcgta accaaagaag ttatcccgcc
    15361 gtactactcg gtgaaagaag tggtgctgcc gttcaataaa ttcccgggtg ttgacccgct
    15421 gttagggcca gaaatgcgct ccaccgggga agtgatgggc gtgggccgca ccttcgctga
    15481 agcgtttgcc aaagcgcagc tgggcagcaa ctccaccatg aagaaacacg gtcgtgcgtt
    15541 gctttccgtg cgcgaaggcg ataaagaacg cgtggtggac ctggcggcaa aactgctgaa
    15601 acagggcttc gagctggatg cgacccacgg cacggcgatt gtgctgggcg aagcgggcat
    15661 taatccgcgt ctggtgaaca aggtgcatga aggccgtccg cacattcagg accgcatcaa
    15721 gaatggcgaa tatacctaca tcatcaacac cacttcaggc cgtcgcgcga ttgaagactc
    15781 ccgcgtgatc cgtcgcagtg cgctgcaata taaagtgcat tatgacacca ccctgaacgg
    15841 tggtttcgct accgcgatgg cgctgaatgc cgacgcgacc gaaaaagtaa tttcggtgca
    15901 ggaaatgcac gcgcagatca aataatagcg tgtcatggcg gatatttttc atccgctaat
    15961 ttgatcgaat aagtaatacg gttctctgat gaggaccgtt ttttttgccc attaattaaa
    16021 tcttttgggg aatcgatatt tttgatgaca taagcaggat ttagctcaca
//
LOCUS       DAJTZL010000090        15961 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.090, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000090 DAJTZL010000000
VERSION     DAJTZL010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15961
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.090"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..453)
                     /gene="spy"
                     /locus_tag="OO544_002486"
     CDS             complement(<1..453)
                     /gene="spy"
                     /locus_tag="OO544_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone"
                     /protein_id="HCR6768058.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPA"
     gene            complement(783..1751)
                     /gene="astE"
                     /locus_tag="OO544_002487"
     CDS             complement(783..1751)
                     /gene="astE"
                     /locus_tag="OO544_002487"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HCR6768059.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGDPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(1744..3087)
                     /gene="astB"
                     /locus_tag="OO544_002488"
     CDS             complement(1744..3087)
                     /gene="astB"
                     /locus_tag="OO544_002488"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HCR6768060.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRLAAKQ
                     GLLKMKTLADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAPVTESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            complement(3084..4562)
                     /gene="astD"
                     /locus_tag="OO544_002489"
                     /pseudo
     CDS             complement(3084..4562)
                     /gene="astD"
                     /locus_tag="OO544_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
     gene            complement(4559..5593)
                     /gene="astA"
                     /locus_tag="OO544_002490"
     CDS             complement(4559..5593)
                     /gene="astA"
                     /locus_tag="OO544_002490"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HCR6768061.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRVDFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(5590..6810)
                     /gene="astC"
                     /locus_tag="OO544_002491"
     CDS             complement(5590..6810)
                     /gene="astC"
                     /locus_tag="OO544_002491"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HCR6768062.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPPDIRHAAYNDINSASALIDDATCAVIVEPIQGEGGVVPASNAFLQGLRELCDRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGGFPVGALLTTEECASVMTVG
                     THGTTYGGNPLASAVAGKVLELINTPEMLNGVKQRHDWFVERLNTINHRYGLFSEVRG
                     LGLLIGCVLNADYAGQAKQISQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAAACEHFVSRGSS"
     gene            7256..8062
                     /gene="xthA"
                     /locus_tag="OO544_002492"
     CDS             7256..8062
                     /gene="xthA"
                     /locus_tag="OO544_002492"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HCR6768063.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            8229..8939
                     /locus_tag="OO544_002493"
     CDS             8229..8939
                     /locus_tag="OO544_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCR6768064.1"
                     /translation="MNAERKFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFIIATLFVLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILVQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            8944..9621
                     /gene="ydjY"
                     /locus_tag="OO544_002494"
     CDS             8944..9621
                     /gene="ydjY"
                     /locus_tag="OO544_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjY"
                     /protein_id="HCR6768065.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            9636..10343
                     /locus_tag="OO544_002495"
     CDS             9636..10343
                     /locus_tag="OO544_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCR6768066.1"
                     /translation="MMKMQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAADLTSIRFRSFFIATGLGQLPTTIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            10343..10891
                     /locus_tag="OO544_002496"
     CDS             10343..10891
                     /locus_tag="OO544_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HCR6768067.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSSDKLLAVAD
                     WRQNPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            10901..12067
                     /locus_tag="OO544_002497"
     CDS             10901..12067
                     /locus_tag="OO544_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR6768068.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTGWAETLPNWRYVDTQLPVREDFSVPTQGAESPWGGAQLTFIARRD
                     VTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPAALKEAPDDA
                     TFARVTAPLWQYLDALHPYLWREGKDFPPSPARMDALLKAGTLRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFREGMIGNVHFVTIPANANASAAAKVVANFLLSPDAQLRKADPVV
                     WGDPSVLDPQKLPDGQREILQSRMPQDLPPVLAEPHAGWVNALEQEWLRRYGTH"
     gene            12040..13576
                     /locus_tag="OO544_002498"
                     /pseudo
     CDS             12040..13576
                     /locus_tag="OO544_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            13576..14229
                     /locus_tag="OO544_002499"
     CDS             13576..14229
                     /locus_tag="OO544_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR6768069.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALL
                     STLKGTARRNAVKDALDRAGLAETYHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRELAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            14296..15603
                     /gene="ynjE"
                     /locus_tag="OO544_002500"
     CDS             14296..15603
                     /gene="ynjE"
                     /locus_tag="OO544_002500"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HCR6768070.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNAWIKQHNLKADAPVALY
                     GNDKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAGARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKLPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPEQQVSFYCGTGWRAS
                     ETFMYARAMGWKNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(15612..>15961)
                     /locus_tag="OO544_002501"
     CDS             complement(15612..>15961)
                     /locus_tag="OO544_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HCR6768071.1"
                     /translation="FYALVPFGFILAAPEQNALAGGWLLFAFIGTGSSFLAFAALAAK
                     HQIDNPGYAHKSFYYLGGLTEGTETILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRV
                     WSGYLTLKSLQRQ"
BASE COUNT         3700 a         4345 c         4162 g         3754 t
ORIGIN      
        1 cgctggacgt tctgtcagac gcttctcaaa attagcatta aattgctttt tctgttccgg
       61 cgtcaggatg ttgtaaattt tgttctgggt ttccatgtgc gccagcatgt tagctttgcg
      121 ctgttcttcc atttttgcga tctgcgcttc agcttttgct ttatcgaagg tatcgctggc
      181 aatgatgtca tgcattgcgc ggcgttcttc cagcggcgga cgtttcatct ggtcacgctg
      241 gcctttcatg atttcgcgga tctgctgttt ctgcgcgtcg gtcaggttca ggtctttgaa
      301 catcatgtcc tgatgcggac cgaacttgcc tttgtggtgc atcatcggct tcgcgtcagc
      361 cggtgctgcg gtagtggtgt ctgcggcatg ggccaggtta gccgcgccaa gagccagggt
      421 agaggcaaca aacagtgcag ttaatttacg catattctat atccttcctt tcagttattt
      481 attacggctt tcttaagtag cgtgccgtgt tgacgagatt aactttactg actttagcgt
      541 caattaatca gagcaacggt aaaacaatga aagtgtaaaa aacacttttg cgccaattat
      601 ggagaaaaaa agaaaatttg atggagagtg atgagagaat attacaacac aatgattttg
      661 cagagattat gaagaactat accggatgac tggtgataaa taaagcaaat aaccaggatt
      721 aatctgtatt aatttataag aaagcaactt aatacccgca gaatgatttc tgcgggtaag
      781 tattagctta ttttttcgag cattaatccc gcgcgtaatc ccaacgctac caacggatta
      841 gggaataaca catactctac atcatgggtt acggtaaaac gttcctctcc gtcctgcgcc
      901 agcaatgttc ctttctcaaa cggcataaaa ttcagcgtgt cacttgccat atgcatttcg
      961 aaggacggcg agtggcgagt aatttgcgaa accacccgat aacggagcgg cggtgttctc
     1021 acgataccga cactctcacc agatagcagc gcagcaattg cgctggcagt tactgcaaac
     1081 tggcgaagat cgttttgccc aaagggcaac gctttgccaa gttccagcgt acaggccagc
     1141 gcgccaaaat gtctggcgct gaaatgggta aacgtaccac caggctcctg atggaacacc
     1201 agcgcctcca gccccgccgc acccagccac gtcagaaatt tctcgtccca gggaatgtcg
     1261 cgttgcggta atacaccgaa ctgaggatgc aaggagccac gaattgcggt atgtagatca
     1321 aggtgccaac gcacagattc tttgccctgg tcataaaaat cttccaggca ctgttccagt
     1381 tcgcgcgccc gacaggtttc tccgctttca gcaaatagct gccaacgacc gccaaacatt
     1441 cggttcatat cgctatggca ataacgtttc ccttgcttca gcgcaggagg atccccgagg
     1501 atcaccagca accgccaacg taacgggatc tcgccgtgag atatcgcgcc aagcaacgcg
     1561 tccagcattt ccacaggtgc cgtctcatta ccgtggatcc ccgcagaaat caccagcgcg
     1621 ccttgcggtg gcgttaatgg cgtcagttcc agcacaccat cgcccagcca gcgccagcga
     1681 acgccgttga tttctcgctc ggtgataacc ggttttttac ccgttaaggt cagagcaaga
     1741 aaattatcca ttgccccctc cctcgcgctg gaacggataa accgaaccga gattcagtaa
     1801 ttggctcaat acatccagtg cttcccgccc ttcgcgcagc aattgcgggt cggccagatc
     1861 ggcagcagta aggcgatcgc ggtagtaacg atccacccag tcattgagcg cattaaacag
     1921 cgtatcgttc atcatcaccg ccggattcac cgcccggcgt tcttcttctg tcaatactac
     1981 ccgcaaccgc aggcacgccg ggccgccgcc attcgccatg ctttcacgta aatcaaagac
     2041 ttttagttcg ctaatcgggt tgtcagcggc aaggagttca ttgagataac cccatactcc
     2101 ggcgtgttcc cgacactcct gaggcagcac caacatcatg gaaccatcat cgcggctcaa
     2161 cagttggctg ttaaacagat aggtagacac cgcatccgac acggaaacct gagttgccgg
     2221 aacttctatc gccataaaac cgttcacccg cgcccgcagg tttgccagta actgtgactg
     2281 gcgagcgaac gcctgttggt ggcaaaacag cacctggcgg ttacttacgg caatcacgtc
     2341 attatgaaaa acaccctggt cgataacgtc cgggttttgc tgggcgaaaa tcacctgttg
     2401 gggattcacc tgattaagcc ttgccaccgc ctcgctggct tcgcgagtct gtcgcgccgg
     2461 ataacgggaa ggccgggtat cattgccttc ttctcgcccg tagacaaaaa gttgcatacc
     2521 cggctcaccg taatgaccgc cgagacgatt gtggtttgcc gccccctcat caccgagcaa
     2581 cgctacctgc ggcaacgccg aatggacgct aaatttctct tcgtcgttaa aaatagcttt
     2641 taacagcgat tcagtgacgg gcgcttccag cgaacggtga aatttattgt tcaggttggc
     2701 aacggtgaga tgcactttac catccagcgt atcggcagat ggcgcgatcg ttgccgcatt
     2761 ggctacccac attggcgaag cggagctgac gctggaaagc cagtgcggtg cctggcgtgc
     2821 aactttttcc agtacctgct catcgctacc actgaatccc aactgacgca gcaccggaat
     2881 aaacggacgc tcgtgcggcg ggatcacggc ctgggggaat cccgcatcgg caagggtttt
     2941 cattttcagt aagccctgtt tcgccgccag tcgcgggtta gacacctgaa aacggtgacg
     3001 ggtagaggct tcattaccaa acgacaggcc agcgtaatga tgcgtcagcc ctaccagccc
     3061 gtcgaaatta acttcccagg cgttcatcgc accacctcat cggaaaaatc cagcccgggg
     3121 ttaagcgttg cgggcaaagt taacgagtcc gactccaggc tcgccatagg ccatgcgcag
     3181 taatctgcgg cataccaggc gctggggcga tggttaccgg atgcaccaat gccgccgaat
     3241 ggcgcggtac tggcagcacc agtaagcggt ttgttccagt taacaatccc cacccgcgcc
     3301 tccagcaaca gttgatcgaa tttttcccgc tcgggggaaa ccagaccgca agagagtccg
     3361 aagcgagtgt tattcgccat tagaatcgct tcctcgaaag aatcataacg ccagacgcgc
     3421 aataacggtc cgaacacctc ttcatctggt acgccagcaa cgcctgtcat ttcaatgatc
     3481 cccggcgtca gcaacgatgt ctctgattgt aataagcgcg gcgcaagcag ggttcgtccg
     3541 cccatcgctt ccagttgctg ctaggcagta accacctgct gtgcggcctg ttcagaaatc
     3601 agcccgccaa taaacggctg cggttcgtca tcccagttgc ccggcgttaa tcgctggctg
     3661 acggcaacca gacgagcaag aaacgcatcg ccctgtgctc cgcttttgag caataaacgg
     3721 cgggcgcagg tgcagcgttg cccggctgtg acaaacgccg actgaatggt cagatggaca
     3781 gccgagtcga tatccgccac ctcatcgata attagcggat tattaccgcc catctcaagg
     3841 gcgagaattt tctccggctg accggagagc tggcgatgca gctggtagcc ggtattggcg
     3901 ctaccggtaa acagcaaacc gtcgagatcc tccagcgcac tcagcgcctg acccgtttca
     3961 cgcccgccct gcaccaggtt cagcacgccc ggcggcaagc cagcctgctg ccataaacgc
     4021 attaccgctt cgccactcca cggtgtcagt tcgctgggtt taaagataat ggtgttacct
     4081 gccagcaatg ccggaacgat atgtccgttc ggcaaatgac cagggaaatt atacggccca
     4141 aacaccgcca gcacgccgtg cgggcgatgt cgcaggctcg ccgcaccgtc cggcatttca
     4201 ctacgctgct cgccggtacg aacgtgatac gccttaattg atatcgcgat tttattgatc
     4261 atcgccgtca cttcggttgc cgcttcccag cgcggcttac ccgtttctct ggcaataatc
     4321 gcggttaatt cggctttatt gctttccagc aatccggcaa agcgttcgac aacgacctga
     4381 cgttcagcca atgagagccg cgcccagcgc ggaaacgccg cacgggctgc ccgacaagcc
     4441 tgttcgacct gagcggcatc ggcatcattg ccttgccata acacctcgcc cgataccgga
     4501 ttacgcttca cacgcgatgc gccctggccc gttatccagt caccgttaat ccataaagtc
     4561 atgctgtttt ctcctctgcg cacaggcgca ccagacgaac gcgatcccct gcgtggcatt
     4621 tgagggcatc cagttgtgcg gcggttaaaa tcaaacgctc ggttgccgga tcggtacgcg
     4681 ccagcaccac gcggaaatgg tgataatttt cattggcgac caggcaggcc ggaaaatcgc
     4741 cctgcgcagg ctgcccttct gccacttcca ccagccgact tttacggatg gcgcgcacgc
     4801 ggtcgatgtc acactcaagc gttggcccgc cgtcgaagat gtcgatatag ttgcggtaac
     4861 gaaaaccttc tttctccagc accgcgcggg caggcgcggt ttgcggatgt acctgaccga
     4921 tgacgtcctg ggcttcctgg gataaaaagt gggtatagat cggatgtttc ggcatcagtt
     4981 ctgcaataaa tgccttttgc ccggtgccgc agagaaaatc gacgcggcta aaatccatcg
     5041 aaaagaagcg tttaccgagg ctttgccaga acggtgaata gccgtgttcg tcaatcaccc
     5101 cgcgcatttc agcaaccact ttgtcattaa acttgtcgcg aaaagccgcc ataaacataa
     5161 agcgcgattt cgacagcaaa tagccgttgc cctctttgcg ccagtccggg tcgagaaaca
     5221 gcgtgcacag ctcgctgctg ccggtgtgat cgttactaag aaacagcgtc ggcaatgcgt
     5281 tatagacatt cagctctttt gaggcgtgaa ccaacgtgcc gacccgatag ttgtaccagg
     5341 gatcgttcag cccaaccgcc acctcaatgg cgcaaatccc cgccacggtg cctgtctcgc
     5401 tatcttccag cacgaacaca tagccctgct cgcttttggg cagttcgcct tgccaggttt
     5461 tgattgccct ttcgatacgc gcggaaagcg tggcttcatt ggcgggaagc gacgtcaggc
     5521 cgccgcccgt tttgctggca agctgcatca gcgccgagac atctgagcgc tcaacgggac
     5581 ggatgaccat catgatgaac ctcggctaac aaagtgttcg caagcagctg caaagcgatc
     5641 cagtccggtc gtcacctctt cttcgctgac attgagcgca ggcgcaaaac gcaccacgtt
     5701 gccacccgca atcagtacca taacgcctgc tttcgccgcc tcctgagaga tctgtttcgc
     5761 ttgtccggcg taatcggcat tcagtacaca gccaatcaac aaacctaagc cgcgaacttc
     5821 actgaacaaa ccgtagcggt gattaatggt attaagacgc tcaacaaacc agtcgtgacg
     5881 ctgtttaacg ccattaagca tctctggtgt gttgatgagc tccagcactt tgcccgccac
     5941 cgccgaggcc agcggattgc cgccataggt ggtgccatga gtgccaacgg tcatcacgct
     6001 ggcgcactct tcggttgtta acaacgcacc gaccgggaaa ccgccgccca gcgctttggc
     6061 ggtagttaac agatcaggtg tcacgccgta gtgcatatag gcatacagtt ccccggtgcg
     6121 cccgacgccg gtttgtactt catcaaaaat caacagcgca ttgtggcggt cacacaattc
     6181 acgcagacct tgtaaaaacg cgttgctggc tggcaccaca ccgccttccc cctggatggg
     6241 ttcgacaatc accgcacagg tggcgtcgtc aatcagcgcg ctggcagagt taatatcgtt
     6301 atatgcagca tgacggatat ctggtggcag tggcgcaaaa tcctgtgaat aggctggctg
     6361 cccacccgca ctgacagtaa acagcgtgcg accatgaaac gcatttttaa acgccacgat
     6421 accgctctta tggctgccgt agcggtcgtg agcgaattta cgcgccagtt ttagcgccgc
     6481 ttcgttggct tccgcaccgg agttacaaaa gaagacgcga tcggcaaacg tggcgtcgat
     6541 caattttttc gccagtcgca gtaccggctc gttggtgtaa ccgttgccgg tatgccagaa
     6601 cttactcgcc tgttcgttca gcgcttcacg cagttccgga tgcgcatggc ccagcgcgtt
     6661 caccgcaatg ccacccgcga agtcgatata ctctttcccc tgctgatccc acaagcgcga
     6721 accttcgcca cgtaccggta taaagggtgc cggagcgtaa acaggtatca tccattcatc
     6781 aaagttttca cgcgtaattg gctgagacat agcgacctct acagtaaata attcgctatc
     6841 tatatgttaa caataagtaa tgtttgcgct gtaaatgtag attgcagggt tcgtgccagc
     6901 cagtgataaa agtgcataaa cggcggaggc taactggaaa tcaaggagtt ataaccaaac
     6961 catatgcatt taaagtgcat ataaagtgaa tacgtttgcg atgtgggtga ataaaaagaa
     7021 taaaaaacgc aatgttatgc agaagtaaaa tataattctg gaattgtgat cattgacgaa
     7081 atttactgga aattactgcg ccattctgac gcagcgcgca ccaaaagcgg gcattttttg
     7141 cgccatcgtt gacatcatta acaaccatcg atcaaatcac ttaacaacag gcggtaagca
     7201 acgcgaaatt ctgctaccat ccacgcactc tttatctgaa taaatggcag cgactatgaa
     7261 atttgtctct tttaatatca acggcctgcg cgccagacct caccagcttg aagccatcgt
     7321 cgaaaagcac caaccggatg tgattggcct gcaggagaca aaagttcatg acgatatgtt
     7381 tccgctcgaa gaggtggcga agctcggcta caacgtgttt tatcacgggc agaaaggcca
     7441 ttatggcgtg gcgctgctga ccaaagagac gccgattgcc gtgcgtcgcg gctttcccgg
     7501 tgacgacgaa gaggcgcagc ggcggattat tatggcggaa atcccctcac tgctgggtaa
     7561 tgtcaccgtg atcaacggtt acttcccgca gggtgaaagc cgcgaccatc cgataaaatt
     7621 cccggcaaaa gcgcagtttt atcagaatct gcaaaactac ctggaaaccg aactcaaacg
     7681 tgataatccg gtactgatta tgggcgatat gaatatcagc cctacagatc tggatatcgg
     7741 cattggcgaa gaaaaccgta agcgctggct gcgtaccggt aaatgctctt tcctgccgga
     7801 agagcgcgaa tggatggaca ggctgatgag ctgggggttg gtcgatacct tccgccatgc
     7861 taatccgcaa acagcagatc gtttctcatg gtttgattac cgctcaaaag gttttgacga
     7921 taaccgtggt ctgcgcatcg acctgctgct cgccagccaa ccgctggcag aatgttgcgt
     7981 agaaaccggc atcgactatg aaattcgcag catggaaaaa ccgtccgatc acgcccccgt
     8041 ctgggcgacc ttccgccgct aatttagcag ctctcctggc tcaaactggg tcaggagaat
     8101 taaccttgag aaaaatcaac aaactgtcag taatgatttg ttgcctgccg tcctttgtta
     8161 taccgtcact gcgtttttag ttgtctgacc acttctctat tatcaagttt gatataggaa
     8221 actccacgat gaatgctgag cgtaaatttc ttttcgcctg tcttattttt gcgctggtca
     8281 tttacgctat ccacgctttc ggtttattcg atctgctcac cgatttaccc cacttacaga
     8341 cactcattcg ccagagcgga ttgttcggtt atagcctcta tattctgtta ttcatcattg
     8401 ccaccctctt tgtgttacca ggaagcatac tggtgatcgc tggaggaata gtttttggcc
     8461 cgctcttagg gacactactc tcattaattg ccgccacgct ggcctcctcg tgctcattcc
     8521 tgctggcgcg gtggctggga cgtgatttac tgctgaaata cgtgggccat agccatacct
     8581 tccaggccat tgaaaaaggc attgcgcgta acggtattga ttttcttatt ctgacccgct
     8641 taatcccgtt gtttccttac aatattcaaa attacgctta cggattaacc accatcgcct
     8701 tctggcctta tacccttatt tcggcactca cgaccctgcc cggtattgtt atttataccg
     8761 tgatggcaag cgatctcgcc aatgaaggca ttacgctgcg ctttatttta caactctgtc
     8821 tggcgggcct ggcgctgttt attctcgtcc agctcgcaaa actctacgcc cgacacaaac
     8881 atgtggatct gtctgcttcg cgccgcagcc cgcttactca cccaaaaaat gaaggataga
     8941 acgatgtcgc aacattattc agtgtcatgg aaaaaaggac tggctgcact ctgcttactg
     9001 gctgttgccg ggcttagcgg ctgcgatcaa aaagagaatg cggcggcaaa agtggaatac
     9061 gacggacttt cgaacagcca accgctgcgc gtcgatgcca ataaccatac ggtaaccatg
     9121 ttggtgcaaa ttaatggtcg tttcctcacc gacgacactc gtcacggtat tgtgtttaaa
     9181 gatggctcca acggacataa atcgctgttt atggcttatg cgaccccgaa agcattttat
     9241 gaagccctga aagaggcagg tggtacgccg ggcgaaaaca tgacgatgga taataaagaa
     9301 acgactcatg tcacaggcag caaactggat atttcggtca actggcaagg ggcggcaaaa
     9361 gcgtattcct tcgatgaagt gattgttgac agtaatggca agaaactgga catgcgcttt
     9421 ggcggtaatt taacggcagc agaagagaag aaaacgggtt gcctggtgtg tctggatagc
     9481 tgcccggtcg gcatcgtcag caatgcaaca tacacttatg gtgcggtgga aaaacgcggc
     9541 gaagtgaaat tcaaaggcaa tgcctcagtt ctcccggcgg ataacacgct ggcaacggtt
     9601 acctttaaaa tcaccgaata aagccaggat aaaggatgat gaagatgcaa tcgcgtaaaa
     9661 tctggtacta ccgtataacc ctcatcatcc tgttgttcgc tatgctgctc gcatgggcgc
     9721 tgcttcccgg cgtccatgag tttatcaatc gcagcgttgc ggcgtttgcc gccgtggacc
     9781 aacagggtat agaacgcttt attcagtctt acggcgcact ggcggcagtt gtctcgttct
     9841 tgttgatgat tttacaggcc attgctgcac cgctacctgc gtttttgatc acctttgcca
     9901 atgcgtcgct gtttggcgcg ttctggggcg gcttgctgtc gtggaccagt tcgatggccg
     9961 gcgcggcgct gtgctttttt atcgccagag tgatgggccg cgaagtggtg gaaaaattaa
    10021 ccggcaaaac cgtgcttgac agtatggacg gctttttcac tcgctacggc aaacacacca
    10081 tcctggtctg tcggttattg ccttttgtcc ctttcgatcc aatcagctat gctgccgatc
    10141 tgacttcaat acgttttcgc tcgtttttta tcgccaccgg gcttggtcaa ttaccgacga
    10201 ctattgttta ttcctgggcg ggcagcatgt taacaggcgg tactttctgg tttgtcaccg
    10261 gactgtttat tctgtttgcc ctgaccgtgg tgatttttat ggcgaagaaa atatggcttg
    10321 agcgccagaa gaggaatgac tgatgggttt accgccgctt agcaaaattc ctttcatttt
    10381 acgtccgcag gcgtggctgc atcgtcgcca ttacggcgag gtgctaagcc cgattcgctg
    10441 gtgggggcgg atcccgttta tcttttattt ggtgtcaatg tttgttgggt ggctggagcg
    10501 caaacgctca ccgctcgatc cggtagtgcg atcgcttgtc agcgcgcgca ttgcgcaaat
    10561 gtgcctgtgt gagttttgtg tggatatcac cagtatgaaa gtcgccgagc gtaccggcag
    10621 cagcgataaa ctgctggcag tggctgactg gcggcaaaac ccgctcttta gcgatgaaga
    10681 acggctggcg ctggagtacg ccgaagccgc aagcgtcaca ccgccaacag tcgatgatgc
    10741 cctgcgtacc cgactggctg cgcattttga cgctcaggcg ctcaccgaac tgacggcatt
    10801 gatcggcctg caaaacctgt cggctcgttt taattctgcc atggacattc ccgctcaggg
    10861 gctgtgccgt attccggaaa aacgttctta aggagagaag atgcgccatt gtgggtggtt
    10921 gctgggattg ttatcgctgt tttctttggc aacacatgcc agtgactggc aggaaattaa
    10981 aaatgaggcc aaagggcaaa ccgtctggtt taacgcctgg ggcggcgata ccgcaattaa
    11041 ccgctatctc gactgggtta gcggcgagat gaaaacccat tacgctataa acctgaagat
    11101 tgttcgtctc gcggatgccg cagacgcggt gaagcgcatt cagaccgaag ctgctgccgg
    11161 tcgtaaaacg ggcggctcgg tggatctgct ctgggtgaac ggcgaaaact tccgcacctt
    11221 aaaagaggcc aatttattac aaacgggctg ggcggagact ctgcccaact ggcgctatgt
    11281 cgacacacag ctgccggtgc gggaagattt ttcagtgccg acacaaggtg cggaatcgcc
    11341 ctggggcggc gcacaactga cgtttatcgc ccgccgcgat gttacgccac agccaccaca
    11401 aacgccgcaa gccttactgg agtttgctaa agccaatccc ggcacggtta cttatccgcg
    11461 cccaccggac tttaccggca cggcgtttct tgaacagttg ctgattatgc tgacgccaga
    11521 tcccgccgca ttaaaagaag cgccggacga tgcgactttc gcccgtgtca ctgcgccctt
    11581 gtggcaatat cttgatgcac tgcatccgta tttgtggcgc gaaggaaagg atttcccgcc
    11641 ttcgcccgcg cggatggatg ctctgctgaa agccggcaca ttgcgcctgt cgctgacctt
    11701 taaccccgcg catgcgcagc aaaaaatcgc cagcggcgat ttgccggcaa gcagttacag
    11761 ttttggcttt cgcgagggga tgattggcaa cgtgcatttc gtcaccattc cagccaacgc
    11821 gaatgccagt gctgcggcga aggtagttgc caatttcctg ctctcacccg atgcgcaact
    11881 gcgtaaagca gatcccgttg tctggggcga tccttctgtt ctcgatccgc aaaaactgcc
    11941 tgacgggcag cgcgaaatat tgcaatcaag aatgccgcag gatctgccgc cggtactggc
    12001 tgaaccgcac gcaggatggg tgaatgcact ggaacaagaa tggttacgcc gttacggtac
    12061 gcattaatct ttttgctgtg ggcgatggtg gcggtgattt atgcaccgct gatcccggca
    12121 gctctcacgc tgatatcgcc tgcgttgtcg ttgacacact ggcaggcgtt atttgccgat
    12181 ccacagttac cgcatgcatt actggcaacg ctggtatcga caaccatcgc ggcggtcggg
    12241 gcattgttga ttgccctgct ggtgattgtg gcgctatagc ctgggccgaa atggcagcgt
    12301 atgtgcgccc gtctgccgtg gctgctcgcc attccccatg tggcttttgc caccagcgcc
    12361 ctgctgctct ttgctgacgg agggctgcta tatgactatt tcccgtattt cactccgcca
    12421 atggaccgat ttggcatcgg tctgggcctc acccttgcag tgaaagaaag cgcatttctg
    12481 ctgtggatct tagcggcagt attgagcgaa aaacggctgt tgcagcaggt cattgtgctg
    12541 gattcgctgg gctacagccg ctggcaatgc ctgaactggc tgctgttgcc ctccgtcgcg
    12601 ccttcgctgg caatggcgat gctggcgatt gttgcctggt cgctttcggt cgtggatgtg
    12661 gcgattattc ttgggccagg taatccaccg acgctggcag taattagctg gcagtggtta
    12721 actcagggcg acgcggatca acaaacgaaa ggcgcactcg ccagtctgct gctgatgctg
    12781 ttactcgccg cctacgtttt gctgagctat ctgctatggc gcagctggcg gcgcactatt
    12841 ccccgcgtag atggcattcg caatgcctgc cacaccttta ttaccaggca ctacgctggc
    12901 gagtttttta cccttaaccg gtgtgctgtg tgtggttctg ctggcgatcc tcgcggatca
    12961 gtcgacgatc aatagtgaag cactcatcaa cagtctgaca atggggctgg tagcaacatt
    13021 catcgctttg ctcctgttac tgctgtggct ggaatggggg ccacagcgtc gccagttgtg
    13081 gctatggttg cccattctat tgcccgatct gccactggtg gcgggccagt acacgctggc
    13141 gctatggctg aacctggatg gaagctggac ggcggtggtc tgggggcatc tgctgtgggt
    13201 aatgccgtgg atgctgttta tcctgcaacc cgcctggcag cgcattgatt cacggttaat
    13261 tttgattgcg caaacactgg gctggtcgcg ggccaaaatc ttcttttacg tgaaatgccc
    13321 actcatgttg cgccctgtgc tgattgcctt cgcggtggga ttttcagtca gtattgcgca
    13381 gtatatgccg acgctgtggc tgggcgcggg gcgttttccg acgctcacca ctgaggcggt
    13441 ggcattaagc agcggcggca gcaacggtat tctcgccgcc caggctttat ggcaactgtt
    13501 gttaccgctt attatttttg ccctgaccgc gttagtcgca aaatgggtag gttatgtcag
    13561 acaaggactc cgctaatgct ctgcgtgaaa aatgtttcgc tacgtttacc agaaagccgc
    13621 ttgctgacaa acgttaactt tacggttgat aaaggtgaca tcgtcacgtt aatggggccg
    13681 tctggctgtg gaaaatccac tctgttttca tggatgattg gtgcactggc tggacagttt
    13741 tcttgtacag gtgagctatg gctcaatgag caacggattg acatgctacc caccgcacag
    13801 cgtcagattg gcattctttt ccaggatgca ctgttatttg accagttcag cgtcgggcaa
    13861 aatttactgc tggcgctact gtcgacgctc aaggggactg ccaggcgcaa tgccgtgaaa
    13921 gacgcgcttg atcgagcggg actggctgaa acttatcacc aggaccctgc gacattatca
    13981 ggcggtcagc gagcgcgggt ggccctgctg cgcgcccttc tcgctcagcc aaaagcgtta
    14041 ctcctggatg agccattcag ccgtcttgat gtggctctgc gcgataattt tcgccagtgg
    14101 gtgttcagcg aagttcgcga actggcgatc cccgtcgttc aggtaacgca cgatctccag
    14161 gatgttcctg ctgatagttc tgttctggat atggcgcagt ggtcagaaaa ttacaacaaa
    14221 ctgcgataac gcaaagtttt tctcaatgcg tcagttcaga atggcgcact caaaactaca
    14281 atgtcgggat tttcgatgaa acgtgtttct caaatgaccg cgctggcaat ggctttaggg
    14341 ctggcttgcg cttcttcgtg ggccgctgaa ctggcgaagc ctcttacact tgaccagctt
    14401 caacaacaaa atggcaaagc gatagatact cgccccagcg cgttttataa cggctggcca
    14461 caaaccttaa atggcccttc tggtcatgaa cctgccgcct taaacctctc tgccagctgg
    14521 ctcgacaaaa tgagcaccga acagctcaac gcgtggatca agcaacataa cctgaaagcc
    14581 gatgccccgg tggcgctgta cggtaatgac aaagatgtcg acgccgtcaa aacgcgactg
    14641 caaaaagcag gttttacgca tatctccatt ctgagtgacg cgctaagcga accttcccgt
    14701 ctacaaaaac tgccgcactt tgagcagctg gtttatccgc aatggctgca cgacctgcaa
    14761 caaggtaaag aggttacggc gaaacctgcc ggtgactgga aagtcattga agcggcctgg
    14821 ggcgctccta agctttacct tatcagccat attcccggtg ctgactacat cgataccaac
    14881 gaagtggaaa gcgaaccgct gtggaacaaa gtttctgatg aacaactaaa agcgatgctg
    14941 gcaaaacacg gcattcgcca tgacaccacg gtcattctgt acgggcgtga cgtatacgct
    15001 ggagcgcgtg tggcgcaaat tatgctctac gcaggcgtga aagatgtgcg tctgctggat
    15061 ggcggctggc aaacctggtc cgacgcggga ctgcctgttg agcgaggaac gccaccgaaa
    15121 gtgaaagcgg aaccggattt cggcgtgaag ctcccggcac aaccgcagtt gatgctcgat
    15181 atggaacagg cccgtggact gctgcatcgc caggatgcat cgctggtgag cattcgttcg
    15241 tggccagaat ttatcggtac gaccagcggt tacagctata ttaaaccaaa aggtgaaata
    15301 gccggagcac gttggggaca cgctggtagc gactcgacgc atatggaaga ttttcataac
    15361 ccggatggca ccatgcgcag cgccgatgat attaccgcta tgtggaaagc gtggaatatc
    15421 aaaccagagc agcaagtttc attctactgc ggcaccggct ggcgcgcgtc cgaaaccttt
    15481 atgtacgcac gcgccatggg ttggaagaat gtttccgtgt atgacggcgg ctggtacgaa
    15541 tggagcagcg atccaaaaaa tccggtagca accggtgaac gcggcccgga cagtagcaaa
    15601 taacattgca attactgacg ctggagcgac ttcagcgtca gatacccact ccacacccgc
    15661 gtaaaggtcg tcatccagca caacgcaccg aagatccacg caaaccacgc aaaccatgca
    15721 gggaataaac atcccagcac aaacagtaag atcgtttctg tgccttcggt taatcccccc
    15781 agatagtaaa acgacttatg tgcataaccg gggttatcaa tctgatgttt tgctgccagt
    15841 gcagcaaagg cgagaaaact gctgcccgtg ccgataaacg caaacaacaa ccagccgcct
    15901 gccagcgcat tttgctccgg tgcagcaaga ataaagccaa acggcaccag cgcgtaaaag
    15961 a
//
LOCUS       DAJTZL010000091        15926 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.091, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000091 DAJTZL010000000
VERSION     DAJTZL010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15926
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.091"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..168
                     /locus_tag="OO544_002502"
     CDS             <1..168
                     /locus_tag="OO544_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6768072.1"
                     /translation="NRRLDFCADAIRHAADDEKLAGIGFHWGFSDQSHFSTVFKQRFG
                     MTPGEYRRKFR"
     gene            complement(340..669)
                     /locus_tag="OO544_002503"
     CDS             complement(340..669)
                     /locus_tag="OO544_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HCR6768073.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTDINAERKASYQQLAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(674..859)
                     /locus_tag="OO544_002504"
     CDS             complement(674..859)
                     /locus_tag="OO544_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HCR6768074.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(856..3495)
                     /locus_tag="OO544_002505"
     CDS             complement(856..3495)
                     /locus_tag="OO544_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HCR6768075.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGMVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVIAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALISGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLTDPTLAFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHEYELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPINATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            3703..4692
                     /gene="ldhA"
                     /locus_tag="OO544_002506"
     CDS             3703..4692
                     /gene="ldhA"
                     /locus_tag="OO544_002506"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCR6768076.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            4803..5225
                     /gene="hslJ"
                     /locus_tag="OO544_002507"
     CDS             4803..5225
                     /gene="hslJ"
                     /locus_tag="OO544_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HCR6768077.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(5222..5488)
                     /locus_tag="OO544_002508"
     CDS             complement(5222..5488)
                     /locus_tag="OO544_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HCR6768078.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            5762..9286
                     /gene="nifJ"
                     /locus_tag="OO544_002509"
     CDS             5762..9286
                     /gene="nifJ"
                     /locus_tag="OO544_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HCR6768079.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPCVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFSAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQTLPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGKRETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTQPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARKCGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
     gene            9652..10785
                     /gene="ompN"
                     /locus_tag="OO544_002510"
     CDS             9652..10785
                     /gene="ompN"
                     /locus_tag="OO544_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HCR6768080.1"
                     /translation="MKSKVLALLIPALLAAGAAHAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNSAKDGDQSYARLGFKGETQINDQLTGYGQWEYNIQANNTESSKNQSWTRLAFAGL
                     KFADYGSFDYGRNYGVMYDIEGWTDMLPEFGGDSYTNADNFMTGRANGVATYRNTDFF
                     GLVNGLNFAVQYQGNNEGASNGQEGTNNGRDVRHENGDGWGLSTTYDLGMGFSAGAAY
                     TSSDRTNDQVNHTAAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPFGDSDYAVAN
                     KTQNFEVTAQYQFDFGLRPAVSFLMSKGRDLHAADGADNPAGVDDKDLVKYADVGATY
                     YFNKNMSTYVDYKINLLDEDDSFYAANGISTDDIVALGLVYQF"
     gene            10926..11360
                     /gene="uspF"
                     /locus_tag="OO544_002511"
     CDS             10926..11360
                     /gene="uspF"
                     /locus_tag="OO544_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HCR6768081.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            11946..12860
                     /gene="sitA"
                     /locus_tag="OO544_002512"
     CDS             11946..12860
                     /gene="sitA"
                     /locus_tag="OO544_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HCR6768082.1"
                     /translation="MHSIKKVTMLLGCLALTCSIAFQASATEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQ
                     RNADTYKAKITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINAD
                     QQGTPQQVRKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTEN
                     GPVPTYIDLLKVTTSTLVQGIKAGKREK"
     gene            12860..13687
                     /gene="sitB"
                     /locus_tag="OO544_002513"
     CDS             12860..13687
                     /gene="sitB"
                     /locus_tag="OO544_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HCR6768083.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNDSEESIITDDERPFVAHRPSAVQREAR"
     gene            13684..14541
                     /gene="sitC"
                     /locus_tag="OO544_002514"
     CDS             13684..14541
                     /gene="sitC"
                     /locus_tag="OO544_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HCR6768084.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            14538..15395
                     /gene="sitD"
                     /locus_tag="OO544_002515"
     CDS             14538..15395
                     /gene="sitD"
                     /locus_tag="OO544_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HCR6768085.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLTWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAIKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISAKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVVFIMTFTVTSINARTKGNA
                     HTQDLLSPN"
     gene            complement(15596..>15926)
                     /locus_tag="OO544_002516"
     CDS             complement(15596..>15926)
                     /locus_tag="OO544_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HCR6768086.1"
                     /translation="IEAWRTEQEAKPFTFEWNGRIWNAGPNSLGRLYPVVMAAKSDIV
                     RDVMTWGDADNQQVKQSMPELEELAAAMAQAQVERNDEIYRRQRELKEELNSLKDLNS
                     VRNFIVE"
BASE COUNT         3984 a         4022 c         3830 g         4090 t
ORIGIN      
        1 aaccgtcgtc tcgatttttg tgcagatgcg attcgccatg ccgcagatga tgaaaaactg
       61 gcaggcatcg gctttcattg gggattttct gaccagagtc atttttcaac ggtatttaag
      121 caacgctttg ggatgacgcc aggcgagtat cgacgtaaat tccgttaact aattcgcgtt
      181 gtgtgtactg caacgccaat tagttaaaac agatatttga ctatgcattt ttcagggcaa
      241 aaggcaattt gacaggagtt tttgaatatt caccttgttt caggaaaata gatcaatcgc
      301 tttctcctgt agcgttatcc cgatattgcg gttttgctat taaaattttc gcacccattt
      361 accgtttatc ccttgtacat attcccccgg tttggcccga gctacgagct tttgcccggc
      421 gagtttcgcg atatcatcta ccgacacatt attttgcttt gccagttgtt ggtaactcgc
      481 tttacgttcg gcattaatgt cggttactaa tttctctgtt tcagcatccg ttttcagcgc
      541 aaccagataa ccataaaatg tttcacctac ccgcccctga gttctggctt catccagagt
      601 caatgccatt acattgctgc ttaccagccc aacaagaaac gcacaaagaa gtaatgtttt
      661 cttcatcatc gcctcagaaa agatcgctac gagtttcaag cagctcttcg acatctttgt
      721 ctgccttgat gatgatctca tgctcaattt taacgttcat attgatagtg atcggttcct
      781 tgggcgcagc gacttcaata cgcggcgtac agccaaccag cataaaagat gacgtcaacg
      841 cagccagtaa aattttcatt gtttttcctc acactctttt ccttgcggac agtcatttcc
      901 cggcagacgt gcgttctgct ccaaccatgc ctggagatta tcgccaaagc gtaaactgcg
      961 ccacagcgta aaaatattct cttcatgatg gtaattaagg tttaccgtac cgcttttacc
     1021 atcaacgcga ctggtacctg taatgttggc ctgcatggtt aataaaccga gattatctaa
     1081 gttaattttt gtcgatgacc ggctaatttc catatagcgc aaccagttaa ttgctgaacc
     1141 cgcagtcata ttgtctttca ccaccgcatc tgcggtgtct ttatccagcc gcaaggtcat
     1201 tggcccgcta tttgccagcc aaccatcttt cactatccat ttttcgttgt ttagccataa
     1261 cggcaatgca ccactaaatg ccccggacat ggcgaattgt ttcggattga cggcgctaac
     1321 cagttcgctg gatgagagat tattcagccg caacagcgcc gggtcatgtt gcggcatacg
     1381 taattgtttc atcagtacgt taccgcctaa cacatcgacg ctaacatcag tcaacagcaa
     1441 gggttcttct tcagtccacg gataacgccc ttgcaaatca gccgtaatat ttttcgctgt
     1501 caccagatta atcacttcgg caattcgcaa cgtaacgggg ccgcgagtcc ccagatgcca
     1561 ggctccatcg gcaaaacgga aaggcaggac aaaatcgaca ccgttaacct gattatctgg
     1621 catccaggca ctaccgcctt tcaacacgcc gtgtcctccc gcgcggaatc cttgttcagg
     1681 cgcagcagaa aatgcaacct gagcatacag ttccccatcg cgtaagttca tcttccagtc
     1741 gggtggcacc agcggctgga atacagtcag tgattgtttt ggccaccagg cgttaccgcg
     1801 cagacgaata ccgtcccagc gaccatttac ccgaaccggg ccaatctcac cagcatgtaa
     1861 atcgccttta aagaggaaat acgtcggatc gcgcccatcg acgctaaatt ttaaggttga
     1921 tggcggtaac acactgccgc cagtgaacag cgtttgcccg gcgtccagtg acagcgcgcc
     1981 gctgaaggag ggatgctgtg cgtcacgtac ccagacgacg ggcttgtcga gaattaatcg
     2041 cggcttttct accgtcatcg taccgtattg taactggtcg aaaccggtgg agaggtcggt
     2101 cagcgtaatc gtgctgtcat gccattcacc ttttcctgcg acatcccagg tggcatttat
     2161 cggtgtaaaa ctgccttttc cccagtagcg ccactgccag cggcccgcgt cagggagaaa
     2221 atcattcgcc agcccatcca tatgcagcac gaaatcgccc agttcatatt catgcgcctg
     2281 caaaatggcc tgcaaacgtc cgtcaacacc acgttgggtg acttttacac ccgctaaagg
     2341 ccagcggatt tcatcgatgt ccagcgaatc gatgactctt cccttcgaac gtaataacgc
     2401 gcctggctca aaggccagcg ttgggtcagt cagacttcca cttagctgcg cgggtaagcg
     2461 ggcatataaa atgaggtccg cctgtttcgc ttcaccggtc agctgcagag gcagctggct
     2521 gttatccatg cttaattttc ctgggccaaa atttagcacc gcgttgcctt tacccgcttg
     2581 cccctgggtc agcacactca gtcgtccgct gatcagagcg ttctcaagcc ctgcctgcca
     2641 gttgtcgact ttgacaccca gtcggccact caaaggaaaa cctgcatacg gccaactcca
     2701 gcgaccatcg cttacggtca attgttgacg agtaatttgc cacggcaaat cgagcaacgg
     2761 atcgccgtta tcccgtgcca gcacaatcaa ttgcccgcta ttttcctgcc agtccagctc
     2821 tgcatccacc agtgacggtt cctgcggcaa gtttaacgtc gcagtagcat gcccacttac
     2881 aggaagtcca tccggcacga gcggcatagt aaattccccc accagtttaa ccggcggctg
     2941 attttcaaac gcgatgacat ccagttcgct gactgtaagt tgttgccctt tcagctggcc
     3001 ttgaaattta actttttcgc cctgataacg cagttgctga atatcagagg ttaatgcgag
     3061 agagagtttt ccctgccatt cctgccaggg agaaaaaatc agtttatcga tattgatcca
     3121 ggtgttaggc agcatggact gccactgcgc gagggttttt ggagcagctg gcgattgctc
     3181 cgtctgcggc aatttcgcca gacaagcaga atcaagttct accatgccga cgttcaataa
     3241 ccagcggctg ggatgtgaaa ggctggcgtt ggtgatatgc gcaagctgac aatctcccac
     3301 cagataacgg agatcgggaa tgattaaacc tttacgcgta atgcgtgggc tttcatctaa
     3361 tgcaatacgt gtcccgagcg gtagccagat gcccgccagc gtgggaaccc acagcccgag
     3421 cgtcatcagc agcgtcaacg gcacaagaat aatcagtaat aacagcgcga gaacggcttt
     3481 atatttaccc agcatgggta gttaatatcc tgatttagcg aaaaattaag cattcaatac
     3541 gggtattgtg gcatgtttaa ccgttcagtt gaaggttgcg cctacactaa gcattgttgt
     3601 tgatgaattt ttcaatatcg ccatagcttt caattatatt tgaaattttg taaaatattt
     3661 ttagtagctt aaatgtgatt caacatcact ggagaaagtc ttatgaaact cgccgtttat
     3721 agcacaaaac agtacgacaa gaagtacctg caacaggtga acgagtcctt tggctttgag
     3781 ctggaatttt ttgactttct gctgacggaa aaaaccgcta aaactgccaa tggctgcgaa
     3841 gcggtatgta ttttcgtaaa cgatgacggc agccgcccgg tgctggaaga gctgaaaaag
     3901 cacggcgtta aatatatcgc cctgcgctgt gccggtttca ataacgtcga ccttgacgcg
     3961 gcaaaagaac tggggctgaa agtagtccgt gttccagcct atgatccaga ggccgttgct
     4021 gaacacgcca tcggtatgat gatgacgctg aaccgccgta ttcaccgcgc gtatcagcgt
     4081 acccgtgacg ctaacttctc tctggaaggt ctgaccggct ttactatgta tggcaaaacg
     4141 gcaggcgtta tcggtaccgg taaaatcggt gtggcgatgc tgcgcattct gaaaggtttt
     4201 ggtatgcgtc tgctggcgtt cgatccgtat ccaagtgcag cggcgctgga actcggtgtg
     4261 gagtatgtcg atctgccaac cctgttctct gaatcagacg ttatctctct gcactgcccg
     4321 ctgacaccgg aaaactacca tctgttgaac gaagccgcct tcgatcagat gaaaaatggc
     4381 gtgatgatcg tcaataccag tcgcggtgca ttgattgatt ctcaggcagc aattgaagcg
     4441 ctgaaaaatc agaaaattgg gtcgttgggt atggacgtct atgagaacga acgcgatcta
     4501 ttctttgaag ataaatccaa cgacgtgatc caggatgacg tattccgtcg cctgtctgcc
     4561 tgccacaacg tgctgtttac cgggcaccag gcattcctga cagcagaagc tctgaccagt
     4621 atttctcaga ctacgctgca aaacttaagc aatctggaaa aaggcgaaac ctgcccgaac
     4681 gaactggttt aatcttgccg ctcccctgca ttccagggga gcttattcag ataatcccca
     4741 aagacctttc atcttctatt cttaaaatag ccctgagtca gaaactgtaa ttgagaacca
     4801 caatgaagaa agtagccgcg tttgttgcgc taagcctgct gatggcggga tgtgtaagta
     4861 atgacaaaat tgctgttacg ccagaacagc tacagcatca tcgctttgtg ctggaaagcg
     4921 taaacggtaa gcccgtgacc agcgataaaa atccgccaga aatcagcttt ggtgaaaaaa
     4981 tgatgatttc cggcagcatg tgtaaccgct ttagcggtga aggcaaactg tctaatggtg
     5041 aactgacagc caaagggctg gcaatgaccc gtatgatgtg cgctaacccg cagcttaatg
     5101 aactcgataa caccattagc gaaatgctga aagaaggtgc acaagtggat ctgaccgcga
     5161 accagttaac gctggcgacc gcgaaacaga cattaactta taagctggcg gatttaatga
     5221 attaatagct gccacagctc ccggcggcaa gtgactgttc gctacagcgt ttgccgttgg
     5281 gtaatgcaca catcccaatc gccgtaccat ccagttgacg ggcaacagaa agcgaaccgc
     5341 cgatcattgc acaatttgct tctccactac tggacatcga cgcttttaaa cctggcgcta
     5401 cgtgcgccgc agtcgcctgc tgaacaggtt cactactaca cgccgacaac aataaagcgg
     5461 cacaccctac ccaaaacgct gctcgcatct cttcttcctc tgatcttcaa gccaaacgac
     5521 accgccataa ataataggca gcacagaggg cggcgtcgag agctgtcctg cgcgttgccc
     5581 cgcattttta cttttttatg gctatttttt tgccctctgt ttgatcaaaa cattcattac
     5641 gctgatgtgg gggacacaaa agcgaaaatg cagaagaaag ccatttgcta aaattgaagg
     5701 attactactg ggcgcgcagc aatttcgtgc gcccctcatt tgcgcaatgt aagggtgtca
     5761 tatgattact attgatggta atggcgcggt tgcttcggtg gcgtttcgca ccagtgaagt
     5821 tatcgccatc taccctatta cccccagttc cacgatggca gaacaggctg atgcctgggc
     5881 cggaaacggc ttgaagaacg tttggggaga cacaccatgc gtggttgaaa tgcagtcgga
     5941 agcgggtgct atcgctaccg tgcatggcgc tttgcagacg ggtgcccttt caacatcgtt
     6001 tacgtcatcg cagggtttgc tgctgatgat cccgacactg tacaaactgg caggcgaact
     6061 cacaccgttt gtcctgcatg tagcggcacg taccgttgcc acacatgcac tctctatttt
     6121 tggcgatcat tccgacgtca tggcggtgcg ccagacgggt tgcgcgatgt tgtgtgcggc
     6181 aaacgtccag gaagcgcaag actttgctct catttcgcac atcgcgacgc tgaaaagccg
     6241 cgtgccattt attcatttct ttgatggttt ccgcacgtcc cacgaaatca acaaaattgt
     6301 cccgctggcc gatgacacga ttcttgatct catgccgcag gccgaaattg atgctcatcg
     6361 cgcccgggcg ctcaacccgg aacatccggt gatccgtggt acgtccgcca atcctgacac
     6421 ttatttccag tctcgcgaag ccaccaaccc ctggtacaac gcagtctatg accatgttga
     6481 acaggcgatg aatgatttct ctgccgcgac aggtcgtcag tatcagccgt ttgaatatta
     6541 cgggcatccg caagctgaac gggtgattat cctgatgggc tcagccattg gcacctgtga
     6601 agaagtggtt gatgaattgc taacccgtgg cgaaaaagtc ggcgtgctga aagttcgcct
     6661 gtaccgcccc ttctcagcca aacacttgct acaaactcta ccgggatccg tacgcagcgt
     6721 ggcggtactg gacagaacca aagaacccgg tgcccaggca gaaccgctct atctggatgt
     6781 gatgaccgca ctggcagaag cctttaataa tggcaagcgc gaaactctgc cccgtgtcat
     6841 tggtgggcgc tatggtcttt catccaaaga atttggcccg gactgtgtac tggcggtatt
     6901 tgccgagctc aacgcggcta aaccgaaagc gcgctttacg gttggtattt acgatgatgt
     6961 aaccaatctg tccctgccgt tgccggaaaa cacccagcca aactcggcga aacttgaagc
     7021 cttgttttat ggccttggta gtgatggcag cgtttccgcg accaaaaaca atatcaagat
     7081 tatcggtaat tccacgccgt ggtacgcaca gggctatttt gtttacgact ccaaaaaagc
     7141 gggcggcctg acggtttctc accttcgcgt gagcgaacag ccgattcgtt ccgcttatct
     7201 catttcccag gctgattttg ttggctgcca ccagttgcag tttatcgata aatatcagat
     7261 ggctgagcgt ttaaaacctg gcggcatttt cctgctcaac acgccgtaca gcgcagatga
     7321 agtgtggtcg cgcttgccgc aagaagttca ggccgtgtta aaccagaaaa aagcgcgctt
     7381 ctatgtgatt aacgcggcga aaatcgcccg caaatgtggc ctggcggccc gtattaatac
     7441 cgtcatgcag atggcttttt tccatctgac gcaaattctg cctggcgata gcgccctcgc
     7501 agaattgcag ggtgcgattg ccaaaagtta cagtagcaaa ggccaggatc tggtggaacg
     7561 caactggcag gctctggcgc tggcgcgtga atccgtagaa gaagttccgt tgcaaccggt
     7621 aaatccgcac agcgccaatc gaccgccagt ggtttccgat gccgcccctg atttcgtgaa
     7681 aaccgtaacc gcagcgatgc tcgcagggct tggtgatgcc ctccccgttt cggcgctgcc
     7741 gccagacggc acctggccga tgggcaccac tcgctgggaa aaacgcaata tcgccgaaga
     7801 gatccccatc tggaaagagg aactctgtac ccaatgtaac cactgcgtcg cagcttgccc
     7861 acactcagct attcgcgcaa aagtggtacc acccgaagcg atggaaaacg cccctgccag
     7921 cctgcattcg ctggatgtga aatcgcgtga tatgcgcggg cagaaatatg tcttgcaggt
     7981 ggctccggaa gattgcaccg gttgtaacct gtgcgtcgaa gtttgcccgg cgaaagaccg
     8041 tcagaatcca gagattaaag ccatcaatat gatgtcgcgc ctggaacatg tcgaagaaga
     8101 gaaaatcaat tacgatttct tcctcaacct gccagaaatc gaccgtagca aactggaacg
     8161 tattgatatt cgtacatcgc agctgattac accgctgttt gaatattccg gtgcttgctc
     8221 cggttgtggc gagacgccgt atattaaatt gctgactcag ctctatggcg accggatgtt
     8281 gatcgctaac gccaccggct gttcttcaat ttatggcggt aacctgccct ctacaccgta
     8341 taccaccgat gccaacggtc gtgggccggc atgggcgaac tctctatttg aagataatgc
     8401 cgaatttggc cttggtttcc gcctgacggt cgatcaacac cgtgtccgcg tgctgcgtct
     8461 gctggatcaa tttgccgata aaatcccggc ggaattactg acggcgttga aatcagacgc
     8521 tacgccagag gttcgtcgag aacaggtcgc agctttacgc cagcaactca acgatgttgc
     8581 cgaagcacat gaactgctac gtgatgcaga tgcactggtg gaaaaatcaa tctggctgat
     8641 tggtggtgat ggctgggctt acgatatcgg ctttggcggt ctggatcatg tattgagttt
     8701 gacggaaaac gtcaacattc tggtgctgga tacgcaatgc tattccaaca ccggtggtca
     8761 ggcgtcgaaa gcgacaccgc tgggtgcagt aactaaattt ggcgagcacg gcaaacgtaa
     8821 agcgcgtaaa gatcttggcg tcagtatgat gatgtacggt catgtttatg tggcgcagat
     8881 ttctctcggc gcgcagctga accagacggt gaaagcgatt caggaagcgg aagcgtatcc
     8941 gggaccatcg ctgatcattg cttatagccc gtgtgaagag catggttacg atctggcact
     9001 cagccatgac cagatgcgcc aactcacagc taccggcttc tggccgctat atcgcttcga
     9061 tcctcgtcgt gccgatgaag gcaaactgcc gctggcgctg gattcacgcc cgccgtcaga
     9121 agcactggaa gaaacgttac ttcacgagca acgtttccgt cgtctgaatt cgcagcagcc
     9181 agaagtggca gaacagttat ggaaagatgc tgcagctgat ttgcaaaaac gctatgactt
     9241 cctggcacaa atggccggaa aagcggaaaa aagcaacacc gattaaatgc tctggataag
     9301 gattacccaa ttctaaaaaa aagcccggac gactgttcgg gcttgtcttt ttatatgttg
     9361 gaaaatcagt ggcaatgcaa tggcccaaca gaaaataaaa tctgtgacaa taaaggcata
     9421 taacccgcgc agaataacgt atagaaaatt aaaagtataa tttttatttt gtatgtatta
     9481 tcttcgatac attcaccaga cttattatca tttcagaaga attatttgat taaggtttta
     9541 cttaaggcgt aacaaatgat atttatcggc taactgaact tctcctttag gatgttttca
     9601 ctccccggaa tggggataaa aggcaaataa aataactaaa ggatttattc aatgaaaagc
     9661 aaagtactgg cacttttaat tcctgccctg ctcgccgcag gtgctgcaca tgcagccgaa
     9721 gtttataata aagacggcaa caaattagat ctgtatggca aagttgatgg cctgcattat
     9781 ttttctgata attcagcgaa agatggcgat cagagctatg cgcgtctggg ttttaaaggc
     9841 gaaacccaaa ttaacgatca actcactggc tacggtcaat gggaatacaa tattcaggca
     9901 aacaacactg aatcttcaaa aaaccagtca tggacccgtc tggcatttgc cgggctgaaa
     9961 tttgcagatt acggttcttt cgattacgga cgtaattatg gcgtaatgta cgacatcgaa
    10021 ggctggaccg atatgctgcc tgaatttggc ggtgactctt ataccaatgc agacaacttt
    10081 atgactggtc gtgccaacgg cgtcgcgact tatcgtaata ctgatttctt cggtctggta
    10141 aatggtctga acttcgcggt gcagtatcaa ggtaacaacg aaggtgccag taatggtcag
    10201 gaaggcacca acaacggacg tgatgttcgc catgaaaatg gtgacggctg gggtctttcc
    10261 acaacatatg atttaggcat gggctttagc gctggtgcgg catacacctc atctgaccgc
    10321 accaatgacc aggttaacca tactgcggcg ggtggtgata aagcagacgc gtggactgct
    10381 gggctaaaat acgatgctaa caatatttac ctggcaacca tgtattcaga aacgcgtaat
    10441 atgaccccgt ttggcgacag cgattatgct gttgcaaaca aaacccagaa ctttgaagtc
    10501 actgcacagt accagtttga ttttggtctg cgtccggcag tctctttcct gatgtctaaa
    10561 ggccgtgacc tgcacgctgc cgatggtgca gacaacccgg caggtgttga tgataaagat
    10621 cttgttaaat acgccgatgt tggcgcgact tactatttca ataaaaacat gtccacttat
    10681 gttgactata aaatcaacct gttggatgaa gatgacagtt tctacgctgc caacggcatc
    10741 tctaccgatg atattgtcgc tttaggtctg gtttatcagt tctaaatcct cctgcccgct
    10801 gttatggcgg gctttttctg cttattcttc ctcctttgat ctaaattaaa aatgtgaact
    10861 ccgtcattac acaaaaagtg tcatctggcg ttacacttta tgcggatact aaaacaggag
    10921 gttttatgaa cagaacgatt cttgtcccta tcgatatttc cgattcagaa ttaactcaac
    10981 gcgtgattag ccacgttgag gcagaggcaa agattgacga tgcagaggtt catttcctga
    11041 cggtaatacc ttcactgcct tactatgcct ctctgggttt agcgtattcc gcagaattac
    11101 cggcaatgga tgacctgaaa gcggaagcca aatcgcaact ggaagagatc attaaaaaat
    11161 ttaaactgcc aaccgacaga gtgcatgtcc atgttgagga aggctcgccc aaagaccgca
    11221 ttctggaatt ggcgaagaag atccccgctc atatgatcat cattgcttcc catcgaccgg
    11281 atatcaccac ttatctgctc ggttccaacg ccgcagctgt agtgcgtcac gcagagtgct
    11341 ccgtgctggt tgtgcgctga cactaacgcc cgcacattgc tgcgggcttt ttgattcatt
    11401 tcgcaaatgt gctgacattt tcccctctaa tccgtaccat acacgccaca gttttatatc
    11461 agatttctta tgctgggcgt tccggcatga cgcatactct tctgatgcca tataacgaat
    11521 tgagtcgctt ttaaatgtcg caaaatcaag aaattagtaa gaaagaacaa tacaacctga
    11581 acaagtaagg gcaaaaatca caactatctg acccataagt attttattta tttttcaata
    11641 tgttaaacat tatcagcgac aacaataagc tacgataacc cactcttttt ttgccccttt
    11701 tttgcccctt ctacagcgtt ttgccccatt tttgccatca acaattttcc tcataaagtc
    11761 atttcaaagc ctaatagctg ctctactttt ttcctcaata tcatgtcgcc agcaggaaac
    11821 aacctaatcc ctgttttagg cctcaaagct gttaatgata atcattttca ttttgtatgg
    11881 gttatgctat aaaacatagc cgttgctatg tttgttctaa ttttgctcac tataggtact
    11941 aaattatgca ctcaataaaa aaagtaacca tgctcttggg gtgcctcgcc ctcacctgct
    12001 cgatcgcatt tcaggcaagt gcaactgaaa aattcaaggt cattacaaca tttaccatca
    12061 tcgcggatat ggccaaaaac gtggctggag atgctgcaga agtctcatcc ataaccaagc
    12121 ctggtgcaga aattcatgag tatcagccta cccctggcga tattaaacgt gcgcaggggg
    12181 cacaactgat tctcgccaat ggtatgaatc tggaattgtg gttccaacgc ttttaccagc
    12241 atctcaatgg ggttccagaa gtaattgtct cttcgggggt gacgccagta gggatcaccg
    12301 aaggacccta tgagggcaaa cctaatcccc atgcatggat gtcgccagat aatgctctga
    12361 tttacgtcga taatattcgt gatgcgttga taaaatacga tccggcaaat gcacaaacct
    12421 accaacgtaa tgccgatact tataaagcca agattaccca aacccttgcc cccctgcgta
    12481 agcagattac ggaactccct gagaatcagc gatggatggt caccagtgaa ggggcttttt
    12541 cttatctcgc acgcgatttg gggctaaaag agctttatct gtggccgatt aatgccgatc
    12601 aacaaggaac accgcagcag gtacgtaagg ttgttgatat agttaagaaa aatcatatcc
    12661 cggcagtctt tagcgagagt acgatttccg ataaaccagc gcgtcaggtt gcgcgtgaaa
    12721 ccggcgcaca ctacggcggt gtgctctatg tcgattccct gagcacagaa aacggcccgg
    12781 tacctacgta tatcgacctt cttaaggtta ctaccagtac gctggtacag ggaattaaag
    12841 ccgggaaaag ggagaaataa tgatgcaatc tgcaggcatt gtcgttaatg atgttactgt
    12901 cacctggcgt aacgggcaca cagcactgcg cgatgcatcc ttcacagtac caagtggatc
    12961 tattgccgct ctggttgggg taaacggttc cggaaaatcg acgttgttta aagcgattat
    13021 gggatttgtg cgtctgacca gcggaaagat atctgttctg ggtattccca cgcgacaggc
    13081 gctacagaaa aacctggttg cctacgtccc tcagtcagaa gaggttgact ggtcatttcc
    13141 tgtactggtg gaagatgtgg tgatgatggg ccgctatggg catatgggta tgttgcgtat
    13201 agccaaaaag agggatcgtc agattgtcac cgatgcgctg gaacgtgtcg atatggtgga
    13261 ttttcgccat cgtcaaatcg gcgagctttc cggtggacaa aagaaacgcg tctttctggc
    13321 gagagcgatt gcacagcagg gtgatgtgat cctgctggat gaacctttta ccggcgttga
    13381 tgtgaaaacc gaagcgaaaa tcatcagcct gttgagggag ttacgcgcgg aaggcaaaac
    13441 gatgcttgtc tcaacgcaca atctcggctc agtcacgaca ttttgcgatt atacggtcat
    13501 ggtcaaaggc accgtcctgg caagcgggcc gacagacacc acttttacag ccgagaacct
    13561 ggagctggct tttagcggcg tactgcgcca tgtcacactt aacgactcgg aagaaagcat
    13621 tattactgac gatgaacgcc cttttgtggc acatcgaccg tcagcggtgc agagggaagc
    13681 aagatgaacg tgctactgga acccttcagc tatgaatata tgctcaatgc gatgtgggtc
    13741 tcggcaatgg tcggcggtct ctgcgctttc ctgtcatgct atctgatgct aaaaggctgg
    13801 tcgctgattg gcgatgcgct gtcgcactct attgtcccgg gtgttgcggg ggcatatatg
    13861 ctggggcttc cgttttctct gggagccttc ttttccggcg gactggcagc gggcagcatg
    13921 ctgtttctta accaacgcac ccgcttaaaa gaagatgcca tcatcggcct gatcttctcg
    13981 tccttttttg gcctggggct gtttatggtg tcattgaacc cgacatccgt gaacattcag
    14041 accatcgtcc tgggcaatat tctggcaatc gatccggcgg atatcctgca actcactatc
    14101 attggcattc tctcgatcat cgtgcttttt ttcaaatgga aagatcttat ggtgaccttt
    14161 ttcgacgaga atcacgcccg tgctattgga ttacatccgg gcagattaaa aatcctcttc
    14221 ttcaccctgc tgtcggtttc gaccgttgcg gcgctgcaaa cggttggtgc ctttctggtg
    14281 atctgtctgg tggtgactcc cggtgcaaca gcatggttgt taaccgatcg ttttccccgc
    14341 ctgctcataa ttgctgtaac tattggcagt gtaaccagtt tcctcggcgc gtgggtcagt
    14401 tactttctgg acggcgcgac aggtggaatc atcgtggtgg cgcagacact gctgtttctt
    14461 ttagcatttg tatttgcgcc cacccatgga cttttggcaa atcgtcggcg tgcgcataaa
    14521 gcgctggagg atcgctcatg atggcattat tactggagcc actgcagttc acctttatga
    14581 gtcacgcact gctgatttca ctggtagttt ccataccctg tgcgctgctg tcggtctttc
    14641 tggtcctgaa aggttgggca ctgatgggtg acgcgatgag tcatgcggtt tttcctggca
    14701 tcgtgcttac ctggattctg gggttacctc tggctacagg ggcattcgtc gccggagtat
    14761 tctgcgcggt ggcgacggga tacctgaaag acaacagccg aatcaagcaa gatacagtta
    14821 tggggattgt tttttctggc atgtttgccg caggtctgat cttgtatatt gcaatcaaac
    14881 cagacgtaca tcttgaccat attcttttcg gcgacatgct cgggataacc atcggcgata
    14941 taatccagac ggtgattatt gccgggctgg ttacacttgt tattagcgca aaatggcggg
    15001 attttttgct gttcagcttt gattatcaac aggcgcaggt aagcggtttg catacgagat
    15061 ggctgcatta tgggctgctg tgcatggttt ccctgaccat tgtggcgacg ctgaaagcag
    15121 taggtatcat tctctctatt tctctgctca ttgcgcctgg cgccattgca gtactcctga
    15181 cacaacgctt tcatattgcg ctcctactgg cgactggcat atcggtaata gtgtcaatga
    15241 ctggtgtctg gctgtccttt tttatcgaca gcgcaccagc tccgacgatt gtcgtcttat
    15301 tcgcggtcgt gttcatcatg acgtttaccg ttaccagcat caacgcacgc acaaagggaa
    15361 acgcccatac acaggatctg ttatcaccca attagcacaa catccgagga cagcatcgtc
    15421 cgccagccta cagaagtccg gaaagccatg gaaggtcgtt gggaggaggt acgaattcta
    15481 tcatgcaaaa aatacgtaaa atcgataacg cctagaaatc attcaatact cgcactatcg
    15541 aaagtttacc agccaaccgc ggcacgttcc tgcatacgac gtgccgcagc ttctgttatt
    15601 ccacgataaa attcctaacc gaattcaaat ccttcagact attcagctct tccttcagct
    15661 cccgctgacg gcgataaatc tcgtcattgc gctcaacctg tgcctgagcc attgctgccg
    15721 ccagttcttc cagttccggc atcgactgtt tcacctgctg attatcggca tcaccccacg
    15781 tcatcacgtc ccgtacaatg tcggatttcg cagccattac caccggataa aggcggccca
    15841 gtgagttggg gccagcattc cagatacgac cgttccattc aaacgtgaac ggcttcgcct
    15901 cctgttctgt gcgccatgct tcaatt
//
LOCUS       DAJTZL010000092        15871 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.092, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000092 DAJTZL010000000
VERSION     DAJTZL010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15871
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.092"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..1453
                     /locus_tag="OO544_002517"
                     /pseudo
     CDS             1..1453
                     /locus_tag="OO544_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281525.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-mannosidase"
     gene            complement(1541..2512)
                     /gene="lpxM"
                     /locus_tag="OO544_002518"
                     /gene_synonym="msbB"
     CDS             complement(1541..2512)
                     /gene="lpxM"
                     /locus_tag="OO544_002518"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HCR6768087.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(2632..3954)
                     /gene="mepM"
                     /locus_tag="OO544_002519"
     CDS             complement(2632..3954)
                     /gene="mepM"
                     /locus_tag="OO544_002519"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HCR6768088.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAAHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            complement(3970..4902)
                     /gene="znuA"
                     /locus_tag="OO544_002520"
     CDS             complement(3970..4902)
                     /gene="znuA"
                     /locus_tag="OO544_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HCR6768089.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPGAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            4981..5736
                     /gene="znuC"
                     /locus_tag="OO544_002521"
     CDS             4981..5736
                     /gene="znuC"
                     /locus_tag="OO544_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HCR6768090.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            5733..6518
                     /gene="znuB"
                     /locus_tag="OO544_002522"
     CDS             5733..6518
                     /gene="znuB"
                     /locus_tag="OO544_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HCR6768091.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(6665..7675)
                     /gene="ruvB"
                     /locus_tag="OO544_002523"
     CDS             complement(6665..7675)
                     /gene="ruvB"
                     /locus_tag="OO544_002523"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HCR6768092.1"
                     /translation="MIEADRLISAGTTLPEDGADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(7684..8295)
                     /gene="ruvA"
                     /locus_tag="OO544_002524"
     CDS             complement(7684..8295)
                     /gene="ruvA"
                     /locus_tag="OO544_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HCR6768093.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARTDASSETLIREALRAAL"
     gene            complement(8434..8499)
                     /gene="yobI"
                     /locus_tag="OO544_002525"
     CDS             complement(8434..8499)
                     /gene="yobI"
                     /locus_tag="OO544_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HCR6768094.1"
                     /translation="MYIFITHLFTEYVILKYLLPI"
     gene            8572..9174
                     /locus_tag="OO544_002526"
     CDS             8572..9174
                     /locus_tag="OO544_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HCR6768095.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGELLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            complement(9176..9697)
                     /gene="ruvC"
                     /locus_tag="OO544_002527"
     CDS             complement(9176..9697)
                     /gene="ruvC"
                     /locus_tag="OO544_002527"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HCR6768096.1"
                     /translation="MAIILGIDPGSRVIGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(9732..10472)
                     /locus_tag="OO544_002528"
     CDS             complement(9732..10472)
                     /locus_tag="OO544_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR6768097.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            complement(10501..10953)
                     /gene="nudB"
                     /locus_tag="OO544_002529"
     CDS             complement(10501..10953)
                     /gene="nudB"
                     /locus_tag="OO544_002529"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HCR6768098.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQVVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            complement(11071..12843)
                     /gene="aspS"
                     /locus_tag="OO544_002530"
     CDS             complement(11071..12843)
                     /gene="aspS"
                     /locus_tag="OO544_002530"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HCR6768099.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTVLAELSIQVVKKAENN"
     gene            13153..13719
                     /locus_tag="OO544_002531"
     CDS             13153..13719
                     /locus_tag="OO544_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR6768100.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDAPSPAKVLPENWWQHPAALGATDSDIEIIKR
                     QWGAFYGTDLELQLRRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAS
                     AEQHNNSINHIYPRIARVRSVEEILHAL"
     gene            14074..14322
                     /locus_tag="OO544_002532"
     CDS             14074..14322
                     /locus_tag="OO544_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HCR6768101.1"
                     /translation="MRIEICIAKEKMTKMPTGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            14923..>15871
                     /locus_tag="OO544_002533"
     CDS             14923..>15871
                     /locus_tag="OO544_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR6768102.1"
                     /translation="MLPTNNNHRLISNSFSTYSIDTSRAYENYLTHWTEWKNNRIQEE
                     QRDIAFQRLVSCLQNQETNLDLSELGLTTLPEIPPEIKSINISKNNLSLISPLPASLT
                     QLNVSYNRLIELPALPQGLKLLNASHNQLITLPTLPISLKELHVSNNQLCSLPVLPEL
                     LETLDVSCNGLAVLPPLPFSLQEISAIGNLLSELPPLPHNIHSIWAIDNMLTDIPYLP
                     ENLRNGYFDINQISHIPESILNLRNECSIDISDNPLSSHALQSLQRLTSSPDYHGPQI
                     YFSMSDGQQNTLHRPLADAVTAWFPENKQSDVSQIWHAFE"
BASE COUNT         4033 a         4028 c         3638 g         4172 t
ORIGIN      
        1 attttttata actattacta ataatatgac atcaattctt cgcagtgagt ttgatgaaat
       61 atggaaagcg atgctgacct tgcaattcca tgacatcctt cccggctcat gtatttcaag
      121 agtttatcat gaaactgaaa aggaatatct taaactagaa gcaaaaaccg agaaaattat
      181 ttctgatgcg cagtctactt tattaagtaa aattgacact tcttcttata aagatccgca
      241 cattttgttt aataccacct gttttgctcg taacgaatgg atcaatataa acaacaactg
      301 gctcaaagca cgagtaaact cttatggtta tgctgtgatt gttccaaaga ataagatcgt
      361 aaatggtctg aaggcagaat ctcaaagtat tgaaaataat tacattaaac ttcttttttc
      421 agaaagcggt gatttaattt cgctatatga taaaagatat ggtaaagaat acattactga
      481 aaatatgcac agtgagatcc gtgcatacca cgaagatgca ggttttttcg cggcctggga
      541 ttttgcttca aattatcgtg atggggaatc atatgtttta ctcgctgaaa aaatgacaac
      601 tgtgatcagc gggcctaaaa ctacgatgac gctgatttat cactacaatt cctcttatct
      661 tcgtttagcc tttaccctga cacaggatag tccacgcgtt gatgtccaaa cgtttattga
      721 ttggcacgaa cctaatgttt ctctcaaagt aaaattcccg gtgtcagtac aaacatctct
      781 tgcacaatgt caaattcagt tcggtgtaat tgacagacca acgcatagcg atgattcgtt
      841 cgcttttgcc aaagatgaaa taccagcaca tcactgggtc gacctttctg atcgagaaac
      901 tggcatagct ttaatagcaa aaaacaaata tggctatcgt gtcaaagacc agacgcttga
      961 attaactttg ctgcgttcgc agcataaacc tggggaagtt gttaagacag acaattatga
     1021 taatttcctg attaataatt ttgcagatat tggtaaacaa aaattcatat tctccttatt
     1081 tcctcatcat ggagattacc gtgtgggtgg tgttattaat caagcctata taatgaatca
     1141 tcccttacag gtcgtccctg tcaaaccgca cccgggtgaa ttaccccctt cactatcatt
     1201 ttttgctatc gatactaaca gcgttatcat cgaaacaata aaattagctg aagacggcga
     1261 cggtataatc gtaagattat atgagagtca tggattagaa atttcagcca tgctttcgtg
     1321 agtatgtaat tatcactacg ttcaatatgt cgatcttcaa gaaaataagt tagatgacag
     1381 tttttattgc aatcaatatt gcaatcttga gttcaaacct tttgaaatta tcacgctacg
     1441 attaactcaa tagcgtacta acctaaaaac tcaaaatagt gggaacactc attgagattt
     1501 tttatcgagt acagcctctc aataatttga gaggcttttt ttatttgata ggataaagat
     1561 ctttacgctt atacggttgg atttcgcccg gtttgcgagt tttcagcaat tttaatatcc
     1621 aggtgtattg ttctggtcgc ggaccaacaa aaatctcgac ttcttcattc atccgccgcg
     1681 caatcgtatg atcatccgcc tctaacagat catccatcgg tgggcgcacc tgaatcgtca
     1741 gacggtgcgt cttgccatca taaatcggaa acagcggaac aacgcgcgca cggcacactt
     1801 tcatcaaacg accaatcgcg ggcaacgtcg ctttataggt ggcaaagaaa tccacaaatt
     1861 cgctgtgctc tgggccatga tcctgatcgg gtaaataata tccccagtac ccctgacgta
     1921 ccgactggat gaatggttta ataccgtcat ttctcgcatg cagacgaccg ccaaagcgac
     1981 gacgcaccgt gttccagaca taatcaaaaa ccgggttgcc ctgattatgg aacatcgctg
     2041 ccattttctg cccttgcgag gccatcagca tggcaggaat atcgacggcc caaccgtgcg
     2101 gcaccagaaa gataactttc tcgttattac gcctcatctc ttcgatgatc tccagccctt
     2161 gccagtcaac gcgcggctga attttctccg gtccgcgtat tgccaactca gccatcattg
     2221 ccatcgcttg cggcgcggtg gcaaacatct catcaacaat cgcttcgcgt tcagcttcac
     2281 tacgttctgg aaagcagagc gacagattga ttaacgcacg acggcgtgag ctttttccca
     2341 gtcgtccggc aaaacgtccc agccgtgcca gaatgggatc acggaacttt ggcggcgtta
     2401 aagcgatacc cgccatcgct gctacaccca gccatgctcc ccagtagcgc gggtggcgaa
     2461 aggatttatc aaactcagga atgtattcgc tattattttt tttcgtttcc atgcttttcc
     2521 agtttcggat aaggcaaaaa tcaatctggt gatagtatag cggcgcaact tgccccgcac
     2581 caaataaaaa aagccgatac tgactgcgta ccggctgcga atggatgtta attaatcaaa
     2641 ccgtagctgc ggcacaatct ctttggcctg cgccaggaat tcgcgacgat cggagccggt
     2701 cagcccttcg gtacgcggca gttttgccgt cagcgggttt acggcctgct ggtttatcca
     2761 tacttcatag tgcagatgcg gcccggttga acgtccggta ttaccggaaa gcgcgatacg
     2821 gtcgccacgt ttcaccttct gtcccggttt caccagaatc ttgcgcaagt gcatataacg
     2881 cgtggtgtag ctgcgaccat gacgaatagc cacataataa cctgctgcgc cactgcgttt
     2941 ggcaaccacc acttcaccgt cacccactga aagcactggc gtaccctgcg gcatggcgaa
     3001 atcaacacct ctgtgtgctg caacgcgacc ggtcaccgga ttagtacgac gcgggttaaa
     3061 gttagacgag atacggaact gtttcgccgt cgggaatcgc aagaatcctt tcgccagacc
     3121 agtaccgtta cgatcgtaga atttgccatc ttcagcgcgg atagcgtaat aatctttacc
     3181 ttctgaacgc aaacgtacgc ccagcagctg gctttgctca cgtttaccat caagcatttc
     3241 tcgagacatt aacaccgcaa attcatcgcc ttttttcagt ttgcggaaat ccatttgcca
     3301 ctgcatggct ttaatcactg cgctcacttc ggcgctggtt aaaccggcgt ttctggcgct
     3361 ggcaacaaag ctccccccga cggtaccttt cagcagattg ttaacccact ctccttgctg
     3421 catttcgctg gtcattttaa aaccgttagc ggcagtacgg tcataggttc gggtttcacg
     3481 acgagacact tcccaggtga ggcgctgcag ttcgccgtcc gcggttaatg tccaggagag
     3541 ttgttgaccg attttcaggt tacgcaattc tttgtcggcc gcagccagtt gggtgatatc
     3601 acccatatca ataccatact gattgagaat gctgcttagc gtatcgccag tggaaacaac
     3661 atattcatgc acgcccgctt caccggcgat tttgtcatcc agttcgtcct ggggaatggc
     3721 ttcatcttct tgtgcagctt gatcaatcgg ctcactggct tcaggtaaga gcgaacgaat
     3781 ttcgttctgt tccagctcaa tggttttgac aattggcgtg gcatcgcggt gataaacata
     3841 gggccgccag acagcgacgg ccagagtaag aacggtgagc gaccccaaca taacgcggtg
     3901 tggtcgcggt aaattattaa acgccagggc gacagagcgg gctatctgtt gcacgtaatc
     3961 acttcctcat taatctcctt tcaggcagct cgcatactgg ttggctaatt gactcaggaa
     4021 ttctgaatag cttgttttac ccagtttgat attcgtcccc aggggatcca acgttcccat
     4081 acgaacggat gtccctcgtg cgacgctctc aacgaccgct ggcctgaact gtggctcagc
     4141 aaaaacgcag gttgcttttt gctcaaccaa ctgtgttctt atttcatgta aacgctgcgc
     4201 gccaggttga atctcagggt taacggtaaa atgaccaagc ggtgtcagtc cgaactgttt
     4261 ttcgaaatag ccgtaagcat cgtgaaaaac gaaataacct ttccccttga gcggcgcgag
     4321 ctcgttacca acctgcgttt cggttgaggc taattgtgcc tcaaaatcct tcaggttggc
     4381 gtcaagtttg gctcgacttt gcggcataag ttccactaat tttccatgga ttgcaaccgc
     4441 tgtagcccgc gctatctctg gggaaagcca aagatgcatg ttgaaatcgc cgtgatggtg
     4501 atcttcgtca cttttttccg cgtggtcgtg atcatcatca tcgccgtgaa tacttttcat
     4561 cagtagcggt ttcacatctt caagctgcgc aatcgttacc tgcttcgctc ctggtaattt
     4621 gcttaccggt ttttgcataa acgcttccat ctccgggcca acccaaacga ctaagtccgc
     4681 gttctgtaag cgttttacat ccgatgggcg cagtgaataa tcatgttctg aagcgccgtc
     4741 aggaagtaaa acctctgttt ctgttacccc atcagcaatg gcagaagcga tgaacccaac
     4801 gggtttaagc gaagcgacaa cggcggcatc tgcggcctgt gttgcacctc cccagagagc
     4861 ggcggataat gctgcgaaaa gaagcgtttt tttatgtaac ataatgcgac caatcatcgt
     4921 aatgaatatg agaagtgtga tattataaca tttcatgact actgcaagac taaaattaac
     4981 atgacaagtc tggtttcctt ggaaaatgtc tcggtttctt ttggccaacg ccgcgtcctc
     5041 tctgatgtgt cgctggaact taaacctgga aaaattttga ctttacttgg gccaaatggc
     5101 gcaggtaagt cgacactggt acgggtagtg ctcgggctgg taacacccga tgaaggggtt
     5161 atcaagcgca acggaaaact gcgcatcggc tatgtaccgc agaagctgta tctcgacacc
     5221 acgttgccac tgaccgtaaa ccgtttttta cgcttacgcc ctggtacaca taaagaagat
     5281 attttgcctg cactgaaacg tgtccaggcc gggcacctga ttaacgcacc gatgcaaaag
     5341 ctctctggtg gcgaaacgca gcgtgtactg ttagcgcgag cattgttaaa tcgcccgcaa
     5401 ttattagtgc tggatgaacc cactcaaggc gtggatgtaa atggccaggt ggcgttatat
     5461 gaccttattg accaactgcg tcgtgaactg gattgtggcg ttttaatggt ttctcacgat
     5521 ctgcatctgg tgatggcaaa aaccgatgaa gtgctgtgcc tgaatcacca catttgttgt
     5581 tccggcacac cggaagttgt ttccctgcat ccggagttta tttcaatgtt tggtcctcgt
     5641 ggtgctgaac aactgggtat ctatcgccat catcataacc atcgtcacga tttacaggga
     5701 cgaattgttt tgcgtcgggg aaatgatcgc tcatgattga attattattt cccggttggt
     5761 tagccgggat catgctcgcc tgtgccgcgg gtccgctggg ttcgtttgta gtctggcgtc
     5821 gtatgtctta tttcggtgat acgctggctc atgcctcatt acttggtgtc gcgtttggtt
     5881 tgttgctgga cgtgaatcca ttctatgcgg tgattgccgt tacgctgctg ctggcgggcg
     5941 gtctggtatg gctggagaag cgtccacagc tggcgatcga cacgttatta gggattatgg
     6001 cgcacagtgc cctgtcgctg ggcctggtgg tcgttagtct gatgtctaat attcgtgttg
     6061 atttgatggc ttacctgttc ggtgatttgc tggcagtgac gccagaagat ctcatctcta
     6121 ttgcgattgg cgtggtcatc gtggtggcta ttttgttctg gcaatggcgc aatttgctgt
     6181 cgatgacgat tagcccggat ctggcgtttg ttgatggtgt gaaattacag cgcgtgaaat
     6241 tgttgttgat gctggtgacg gcattgacga ttggtgtagc gatgaaattt gtcggtgcgt
     6301 tgattattac ttcgttgctg attattcctg cagctactgc gcgtcgcttt gcccgtacgc
     6361 cggaacagat ggctggtgtc gctgttttgg tggggatggt ggcagtaact ggcggtttaa
     6421 ccttttccgc gttttacgat acgccggcgg gtccgtcggt agtgctatgt gctgcactgt
     6481 tatttattat cagtatgatg aaaaagcagg ccagctaatc tgtcgctgaa cacatttgtc
     6541 ggatgcggcg cgagcgcctt atccgaccta cggttcgcta tctctggtag gccggataag
     6601 acgcgaatag cgtcgcatca ggcacactgc caatgtcgga tgcggtgcga gcgaccaatc
     6661 cgacttacgg catttctggc ggcgttatgc caaagtgatt ccacgcccgc gtcgtcgcca
     6721 tacgcccacg cggtgtacgc tgcaaaaagc cttgctgaat cagataaggc tccagcacat
     6781 cctcaatggt ttcacgttct tcgccaatgg ctgccgccag gttatccaga cctaccggcc
     6841 caccaaagaa cttatcgatt accgccagca acaatttgcg gtccatataa tcgaaacctt
     6901 cagcatcgac attcaacata tccagcgcct gagcagcgat atctgccgag atggtgccat
     6961 cgtgcttcac ttcggcgaaa tcacgcactc gacgcagcag acggttggca atacgtggcg
     7021 taccgcgagc gcgacgagca acttccagcg cgccgtcatc actcatctca agccccataa
     7081 agcgtgcgct gcgactgacg atatattgca gatccggcac ctgataaaac tccagacgtt
     7141 gcacaatacc aaaacggtcg cgcaacggtg atgtcagcga acctgcgcgc gtggttgcac
     7201 caatcagggt aaacggcggc aaatcaattt taatggagcg tgccgccgga ccttcaccaa
     7261 tcatgatatc cagttggtag tcttccattg ccgggtacag cacttcttca acaactggcg
     7321 atagacggtg gatctcatca ataaacagca cgtcatgcgg ttcaaggtta gtgagcatcg
     7381 ctgccagatc gcccgccttt tccagcaccg gaccagaagt cgtgcgtaaa ttaacgccca
     7441 tttcattggc gacaatgttg gcaagcgtag ttttacccaa ccccggagga ccaaaaatca
     7501 acagatgatc gagggcatcg ccgcgcagtt tcgctgcttt gatgaaaatc tccatctgtg
     7561 aacgaacctg cggctgacca acatactctt ccagtaattt agggcgaatg gcgcgatctg
     7621 ccccatcttc cggcaaagtg gtaccggcag aaatcagacg gtctgcttca atcatccttt
     7681 acctcataac gcggcgcgta gggcttcgcg aattaatgtt tcactgctgg cgtcagtgcg
     7741 agcgattttg ctcaccatgc ggcttgcttc ttgtggttta tagcccagcg ccaccagcgc
     7801 ggcaaccgct tcctgttcag catcgtcggt tgccgggctg gcaggcgacg tgagcaccag
     7861 atcggcggct ggcgtaaaga gatcgccgtg caaaccttta aatcggtctt tcatttcgac
     7921 aattaaacgc tcggcggttt ttttaccaat gcccggcagt ttcaccagtg ctcccacttc
     7981 ttcacgttca acggcattaa cgaactgctg cgctgacatt ccggagagga tcgccagcgc
     8041 caacttcggg ccgacgccgt tggttttgat taactcttta aacaacgtgc gctcttgttt
     8101 attgttaaaa ccgtacagca gttgcgcgtc ttcacgcacc acaaagtggg tgaaaacgat
     8161 cgcttcctga cccgcttcag ggagttcata aaaacaggtc atcggcatat gcacttcata
     8221 gcctacgccg cccacttcaa ttaacaccag cgggggttgt ttttcaatga tgatgcctct
     8281 gagtctgcct atcacatgac gctcctgcgt aatgaatcaa agataatgct gtatgataaa
     8341 aaaatgctgg atagatatcc agcgaaggat gaagaaaact tgcgaggtgt ctcgatgaac
     8401 tgaaaaatgg cacagtataa tttattctac aggttatatt ggaagcaaat attttaatat
     8461 tacatattca gtgaataaat gcgtaataaa aatatacatt gcgcctcctg aaaaaaaata
     8521 aattttttat gctattacgt atattcgtat ctatttcaat ggaatgacaa cgtgaatatt
     8581 aattaccctg ctgaatatga aattggagat atcgtcttta catgtatagg tgctgcctta
     8641 tttggtcaaa tatcagctgc atcaaattgc tggagtaatc acgtcgggat cattatcggt
     8701 cataacggtg aagacttcct ggttgcagaa agccgggttc ccctttcaac catcaccacg
     8761 ctatctcgtt ttattaaacg ttcgtctaat caacgctatg ctataaagcg attagacgct
     8821 gggctgacag aacaacaaaa acaacgaatt gttgaacagg ttccctcccg gctacgcaaa
     8881 ctttaccaca ccggttttaa atacgaatct tcgcgccagt tctgttcaaa atttgttttt
     8941 gatatttata aagaagcgct atgcattccg gtgggtgaaa tagaaacgtt tggagaactg
     9001 ttaaatagca atccgaatgc aaaactcact ttctggaaat tctggttctt aggttctatt
     9061 ccgtgggagc gtaaaaccgt cacgccagcc agtttgtggc atcatccggg tctggtgttg
     9121 attcacgcgg agggagttga aacgcctcag cctgaactga ccgaggcggt ataacttaac
     9181 gcagtcgccc tctcgccagg ttcagccgcg attcgctcat ctgcattgca ttctggctga
     9241 cgtgacaatg ggtaatcgcg atagctaacg catcggcggc atccgcctgt ggattagcgg
     9301 gcagtttcag caaggtgcgg accatatgct gcacctggct tttttcggca ctaccaatac
     9361 ctaccaccgt ttgctttacc tgacgtgccg cgtattcaaa taccggcaat tcctgattca
     9421 ccgccgccac aatcgccacg ccgcgcgcct gccccagttt cagagccgag tcggcatttt
     9481 tggccataaa gacttgttca atggcgaaat aatctggctg gaactgggtg atgatttccg
     9541 tcacgcccgc atagatgagc ttcagacgag acggtaaatc atccactttg gtgcgtatgc
     9601 atccgctacc caggtaggac agttgcctgc ctacctggcg gatgacacca tagccgatca
     9661 cgcgcgaacc cgggtcaatg ccgagaataa tagccatcac gcgtctccgt tttgctgttt
     9721 agcaggcccc atcagagagt tgctgcaacc tcatcagaga tttcaccgtt atggtagact
     9781 tcctgcacgt cgtcgcaatc ttccagcata tcgatcagac gcatcagttt cggtgcggtt
     9841 tctgcatcca tatcagcttt ggtggacggg atcatggaaa cttccgcgct gtctgctttc
     9901 agacctgccg cttccagagc gtcgcgcact ttacccattt cttcccatgc agtgtagacg
     9961 tcaatcgcgc cgtcatcata ggtcacaacg tcttcagcac ctgcttccag tgctgcttcc
    10021 ataatggtgt cttcatcgcc tttctcgaag gagatcacgc cttttttgct gaacagatag
    10081 gcaacggaac catcagtacc gaggttaccg ccacatttgc taaatgcatg acgcacttca
    10141 gcaacggtac ggttgcggtt gtcagacaga cattcaatca tgattgccgt gccgccagga
    10201 ccgtaacctt cgtagatgat ggtttccatg tttgcatcat catcaccgcc cacaccacgt
    10261 gcaattgcgc ggttcagtgt gtcacgggtc atgttgttag acagtgcttt atccaccgcc
    10321 gcacgcagac gcgggttagc gtccggatcg ccaccgccca gcttagccgc ggttaccagc
    10381 tcacgaatga ttttagtgaa gattttaccg cgcttagcat cctgcgcagc tttacgatgt
    10441 ctggtgttgg cccatttact atgacctgcc ataaaaatat ctccagatag ccctgcctgt
    10501 tcaggcagcg ttaattacaa actgttcaat cgcctgccgg ttgctccagg acttagtgag
    10561 cgccgcggca gcaggcgcat caagccactt gtaagccaga tgttcagtga agactacctg
    10621 gcgttcgtgc gggagcgcaa gacagaacca tgattccgta ttacgcgtca cgcccggcgc
    10681 atagcgatga cgtaaatgtg aaaaaatttc aaactctacc gtgcgctgac agtcaattaa
    10741 ggtcagttgt tcagcgacaa catcaatggt gacctcttcc tttacttcgc gcatggcagc
    10801 ttgcggcgcg gtttcaccct cttccacgct gccggttacc gactgccaga aatcgggatc
    10861 gtcacgccgc tgcaacatca gcacccgttt cgtatcttgt gcatagatga ccactaagat
    10921 cgaaacggga cgcttataca ctttatcctt cacgctgcct cttcgttgac tgccttcgct
    10981 catcccattc acatagttat ctatgctcat gggagttcac tcagttgccg ccttgatgca
    11041 acgcgaatga tttcgtgtat ttgagtcata tcagttattc tcagccttct tcacaacctg
    11101 aatgctcagc tcagccagta cagtcgggtt agcaaagctc ggtgcttcag tcatcagaca
    11161 cgctgccgcc gtggttttcg ggaaggcgat aacgtcacgg atattgtcgg tgccagtcag
    11221 cagcatggtc agacggtcaa gaccgaatgc cagacctgcg tgcggcggag taccgtattt
    11281 cagggcgtcg agcaggaagc cgaatttctc gcgctgttcc tcttcgttga tacccagaat
    11341 accaaacacc gtctgctgca tatcaccatt atggatacgt actgaaccac cgcccacttc
    11401 gtaaccattg atgaccatat cgtaagcgtt cgccaccgca ttttccggtg cagctttcag
    11461 ttctgcagca gtcatgtctt tcggtgaggt gaacggatgg tgcattgccg tcaggccgcc
    11521 ttcaccgtcg tcttcaaaca tcgggaagtc gataacccac agcggtgccc atttgctttc
    11581 gtcggtcaga ccaaggtctt tacccacttt caggcgcagt gcgcccatcg cgtcggcaac
    11641 aattttcttg ttgtcggcac cgaagaaaat catatcgcca tcttgcgcgg cagtacgatc
    11701 caggatggct tcgatgattt ctgcattaag gaacttcgct accgggctgt tgataccttc
    11761 cagacctttc gcgcgttcgt taactttgat gtaagccaga cctttcgcgc cgtagatttt
    11821 aacgaagtta ccgtattcgt cgatctgctt acgggtcagc gatgcgccac ccggaacacg
    11881 tagagcggca acacggcctt tcggatcgtt cgccggcccc gcaaatactg caaactcaac
    11941 agatttcagc agatcggcaa cgtcggtcag ttccatcggg ttacgcagat ccggtttatc
    12001 agaaccataa cggcgttctg cttccgcaaa ggtcattacc gggaaatcgc ccagatccac
    12061 gcccttcact tccagccaca aatgacgcac cagcgcttcc atcacttcac gcacttgcgg
    12121 cgcggtcatg aaagaagttt ccacatcgat ctgagtaaat tcaggctgac gatcagcgcg
    12181 caggtcttcg tcacggaagc atttaacgat ctgatagtaa cggtcaaaac cggacatcat
    12241 cagcagctgt ttgaacaact gtggggattg cggcagtgcg tagaatttac ctttgtgcac
    12301 acgagaaggc accaagtagt cacgcgcgcc ttccggcgtg gctttggtca gcatcggagt
    12361 ttcgatgtcg aggaagccgt ggtcatccat aaaacggcgc accaggctgg tgattttagc
    12421 gcgggttttc aggcgctgcg ccatttccgg gcgacgcagg tcgaggtagc ggtatttcag
    12481 acgcgcttct tcggtgttga cgtggttaga gtcaagcggc agaacatctg cgcggttgat
    12541 gatagtcagc gaggacgcca gcacttcaat ttcgccggtc gccatatcgc ggttaatatt
    12601 tttttcgtca cgcgcacgta cggtgcccgt aacctgaatg cagaactcat tacgcagttc
    12661 agaggccagc tttaacgcgt ccgcacgatc cggatcgaaa aatacctgca cgataccttc
    12721 gcggtcgcgc atatcgatga agatcaggct accaagatca cgacgacggt tgacccaacc
    12781 acacagagtc acctgctgcc ccacgtggga caaacggagc tgtccacaat attctgtacg
    12841 catgagatat cccttaactt agctgccggc ggatgccccc tgctgcgcag gtgaccaagt
    12901 cgcagcgtta gctgtatgtc acaactgaat gaaaaaaggc ggctattata ctggaaattc
    12961 tgccgcaccg taagagcctg gcccgcgctg taacgcctcg ttaccacttt atatcgggcc
    13021 tgaaatcaga ctctacgcca gtttgctata aaggtgttgc ccgaactcat aaaaattaac
    13081 aaaatttgtc gttccgccat cggctaatcg cattaaggtg tgaggcacga ttttgttttt
    13141 tcaggagtca gcatgcttga acttaatgct aaaaccactg cgttggtggt gatcgattta
    13201 caagaaggca tcttaccttt tgccggtggt ccacatactg ccgatgaggt agttaatcgc
    13261 gccgggaagc tggcggcgaa atttcgcgcc agcggtcagc ccgtgtttct ggtgcgagtt
    13321 ggctggtctg ccgattacgc cgaagcatta aaacagccgg ttgatgcccc ctcccccgct
    13381 aaagtgttgc ccgaaaactg gtggcaacat ccagctgcat taggtgcaac tgacagtgat
    13441 atcgaaatca tcaaacgtca gtggggtgcg ttttacggta cggatctgga gttgcaactg
    13501 cgccgccgag gtatcgatac aatagtgtta tgtgggatct cgaccaatat cggtgttgaa
    13561 tccaccgccc gcaatgcctg ggaacttggc tttaatctgg tgattgccga agacgcctgt
    13621 agtgccgcca gcgccgagca acacaataac agcattaatc atatctaccc gcgcatcgcc
    13681 cgtgtgcgta gcgtggaaga gatccttcac gcgttatgat ttacatcggt ctaccgcaat
    13741 ggtcgcatcc taaatgggtg cggttgggga tcaccagcct tgaagagtat gcccgccact
    13801 ttaactgcgt gatacgggat ttttaaaaat cactaaagaa cgcccaagag catgtgtttt
    13861 ctttagaata atcaaagcgt tggattgcca tgccatatga aaatcggcaa agttcatatg
    13921 taacaaagtg tcccaatcat gccccaaaat gccccacaac aaatattttt gccccatgca
    13981 tgccccaaaa aatcactttc cctcactctt gacactgttt atccatacag ttaaaaataa
    14041 tactgtataa aaacacagtg tagagggatt tttatgcgta ttgaaatctg catagccaaa
    14101 gagaaaatga ctaaaatgcc aaccggtgct gtggatgcgt taaaggaaga attaacccga
    14161 cgcatcagta aacgttatga cgatgtagag gtgatcgtaa aagccaccag caacgatggc
    14221 ctttctgtta cgcgcaccgc cgataaagat tcagctaaaa cttttgttca ggaaactctg
    14281 aaagatacct gggagtctgc tgacgagtgg tttgttcact aattagcacg taaaatcagt
    14341 aacggctgga aatcattcaa tactcgcact atcggaagtt caccagccag ccgcagcacg
    14401 ttcttgcata cgacgtgtct gcggtctcaa ctccgcccgc ccccgccaat catgattggg
    14461 cagtaccagt acagcactag caaagacagg aaattagcag tctcagcaaa acaccgacca
    14521 gacggtgaga agacaaaaag atacgcaaag atgccgcggt tccagaagaa taaaaaaccg
    14581 ctaatggaca gcctccttaa cacagttttt tatatacatg attcactgat accagtggca
    14641 ttaatagcta ttatatagct tcagctcctt ttttcatacc ttaaggaaaa attgccccct
    14701 cccccaaaaa gtatcgtttt tttacattaa accgatccag tttaggatag tgtaaagcag
    14761 aagttcttca atatataaca tatggagtta atatgcttac acaaactata tattcgcctc
    14821 ctccagaagt tcctgtctac tgctgaaggg aaccaataac attgcaaagc agataaaaaa
    14881 gaatccaaca cctctcaaca taaagaatac gccctgatat ttatgctccc gacaaataac
    14941 aatcacagat taatttcaaa ttcgttctcc acttattcaa tcgacactag ccgtgcatat
    15001 gaaaattatc taacccattg gactgaatgg aaaaataacc gcatacaaga agaacaacga
    15061 gacatcgctt ttcagcgact agtatcatgt ctacaaaacc aagagacgaa cctggacttg
    15121 tctgaattag gcctgacaac attacctgaa atccccccgg aaattaaatc aattaatata
    15181 agtaaaaata atttaagctt aatctcccca ttgcctgcgt cccttacaca gcttaatgtc
    15241 agctataaca gacttattga actgcctgct ttgcctcaag gacttaaatt attgaatgcg
    15301 tcccacaatc aactaatcac actacccaca ctccccatat ctttgaagga gcttcatgtc
    15361 tcaaataatc aattatgttc tcttcctgtt ttaccagaac tactggaaac attagatgta
    15421 tcatgtaatg ggctggcagt tttaccacct ttaccatttt ctttacaaga gattagcgca
    15481 atagggaatc ttcttagtga actcccccct ctacctcaca acattcactc catatgggca
    15541 atcgacaata tgttaaccga tattccatac ctgccggaaa atttaaggaa cggttatttt
    15601 gacataaatc agataagtca tatcccggaa agcattctta atctgaggaa tgaatgttca
    15661 atagatatta gtgataaccc attgtcatcc catgctctgc aatccctgca aagattaacc
    15721 tcttcgccgg actaccacgg cccgcagatt tacttctcca tgagtgacgg acaacagaat
    15781 acactccatc gccccctggc tgatgccgtg acagcatggt tcccggaaaa caaacaatct
    15841 gatgtatcac agatatggca tgcttttgaa c
//
LOCUS       DAJTZL010000093        15617 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.093, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000093 DAJTZL010000000
VERSION     DAJTZL010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15617
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.093"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..215)
                     /gene="tuf"
                     /locus_tag="OO544_002534"
     CDS             complement(<1..215)
                     /gene="tuf"
                     /locus_tag="OO544_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR6768103.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDT"
     gene            complement(286..2400)
                     /gene="fusA"
                     /locus_tag="OO544_002535"
     CDS             complement(286..2400)
                     /gene="fusA"
                     /locus_tag="OO544_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HCR6768104.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2497..2967)
                     /gene="rpsG"
                     /locus_tag="OO544_002536"
     CDS             complement(2497..2967)
                     /gene="rpsG"
                     /locus_tag="OO544_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HCR6768105.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3064..3438)
                     /gene="rpsL"
                     /locus_tag="OO544_002537"
     CDS             complement(3064..3438)
                     /gene="rpsL"
                     /locus_tag="OO544_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HCR6768106.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3564..3851)
                     /gene="tusB"
                     /locus_tag="OO544_002538"
     CDS             complement(3564..3851)
                     /gene="tusB"
                     /locus_tag="OO544_002538"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HCR6768107.1"
                     /translation="MLHTLHRSPWLADFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQISNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3859..4218)
                     /gene="tusC"
                     /locus_tag="OO544_002539"
     CDS             complement(3859..4218)
                     /gene="tusC"
                     /locus_tag="OO544_002539"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HCR6768108.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLPGQKPDAVLARDYIATFKLLGLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4218..4604)
                     /gene="tusD"
                     /locus_tag="OO544_002540"
     CDS             complement(4218..4604)
                     /gene="tusD"
                     /locus_tag="OO544_002540"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HCR6768109.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIVEGHELSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGVVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4604..5326)
                     /locus_tag="OO544_002541"
     CDS             complement(4604..5326)
                     /locus_tag="OO544_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR6768110.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5493..6305)
                     /gene="fkpA"
                     /locus_tag="OO544_002542"
     CDS             complement(5493..6305)
                     /gene="fkpA"
                     /locus_tag="OO544_002542"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR6768111.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6526..6744
                     /locus_tag="OO544_002543"
     CDS             6526..6744
                     /locus_tag="OO544_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HCR6768112.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6793..7383)
                     /gene="slyD"
                     /locus_tag="OO544_002544"
     CDS             complement(6793..7383)
                     /gene="slyD"
                     /locus_tag="OO544_002544"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR6768113.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7478..7678)
                     /locus_tag="OO544_002545"
     CDS             complement(7478..7678)
                     /locus_tag="OO544_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HCR6768114.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(7688..9493)
                     /gene="kefB"
                     /locus_tag="OO544_002546"
     CDS             complement(7688..9493)
                     /gene="kefB"
                     /locus_tag="OO544_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HCR6768115.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9493..10044)
                     /gene="kefG"
                     /locus_tag="OO544_002547"
     CDS             complement(9493..10044)
                     /gene="kefG"
                     /locus_tag="OO544_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HCR6768116.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRNVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     KELASHARAYGDWLANPLSPGGR"
     gene            10175..12088
                     /locus_tag="OO544_002548"
     CDS             10175..12088
                     /locus_tag="OO544_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6768117.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12088..13110
                     /locus_tag="OO544_002549"
     CDS             12088..13110
                     /locus_tag="OO544_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR6768118.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEAPAQARHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPPQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13104..13322
                     /locus_tag="OO544_002550"
     CDS             13104..13322
                     /locus_tag="OO544_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HCR6768119.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13376..14245
                     /locus_tag="OO544_002551"
     CDS             13376..14245
                     /locus_tag="OO544_002551"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HCR6768120.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14300..14704)
                     /locus_tag="OO544_002552"
     CDS             complement(14300..14704)
                     /locus_tag="OO544_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HCR6768121.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15006..>15617
                     /gene="crp"
                     /locus_tag="OO544_002553"
     CDS             15006..>15617
                     /gene="crp"
                     /locus_tag="OO544_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HCR6768122.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTI"
BASE COUNT         3884 a         4245 c         3856 g         3632 t
ORIGIN      
        1 gtgtcatatt caacgtgaga agtgttgatg gtgataccac gagctttttc ttccggcgcg
       61 ttatcgatct ggtcgaatgc acgagcagca ccgccgtagg ttttagccag tacggtggtg
      121 attgcagcgg tcagagtagt tttaccgtgg tcaacgtggc cgatagtacc aacgttaacg
      181 tgcggttttg tacgttcaaa tttttcttta gacacggcta tattccttac tatagtgctc
      241 tccccttcag gagagagcac gggactttgg tattaaccct taggcttatt taccacgggc
      301 ttcaattacg gcctgagcaa cgttactcgg cgcttcatca tacttcagga attccatagt
      361 gtatgatgca cgacctttgg tcagagaacg cagctgagtt gcgtatccga acatttcaga
      421 cagcggtact tcagcgtgga tcttaacgcc agtaacttca gattcctgac ctttgagcat
      481 accacgacga cggctcaagt caccgataac gtcaccggtg ttctcttccg gagtttctac
      541 ttcaaccttc atgatcggct caagcagaac tggtttcgct ttcttaaagc cttctttaaa
      601 ggcgatagaa gcagccagtt taaacgccag ttcagaggag tcaacgtcat ggtaagaacc
      661 gaagtgcaga cgaataccca tgtctactac cgggtagcct gccagcggac ctgctttcag
      721 ctgttcctgg atacctttat caacggccgg gatgtattcg ccagggatta caccaccttt
      781 aatgtcgttg atgaactcgt agcctttcgg gtttgaaccc ggctccagcg ggtacatgtc
      841 gataacaaca tgaccatact gaccacgacc accagactgt ttcgcgtgtt taccttcaac
      901 atcggtaact ttctggcgga tagtttcacg gtaagcaacc tgcggtttac ctacgttcgc
      961 ttcaacgttg aattcacgct tcatacggtc aacgatgatg tcgaggtgca gttcgcccat
     1021 acccgcgatg atggtctggt tagattcttc gtcagtccat acacggaaag acgggtcttc
     1081 tttagccaga cggcccagag ccagacccat tttttcctgg tcagctttgg ttttcggttc
     1141 aactgcgatg gagattaccg gctcagggaa ttccatacgt tccagaatga tcggcgcatc
     1201 cgggtcacac agggtgtcac cagtggttac gtctttcaga ccgatagcag cagcgatgtc
     1261 gcccgcgcga acttctttga tctcttcacg tttgttagcg tgcatctgaa cgatacgacc
     1321 gaaacgctca cgtgcagctt tcacggagtt cagtacggta tcaccagagt taaccacacc
     1381 ggagtaaaca cggaagaagg tcaggttacc aacaaacggg tcggtagcga ttttgaacgc
     1441 cagtgcagag aacggctcgt catcacttgc gtgacgctca gccggagtgt ctttaccgtc
     1501 gtccaggata ccgttgatcg caggtacgtc aaccggggat ggcaggtaat caattaccgc
     1561 atccagcatc gcctgaacac ctttgttctt gaacgcagaa ccacaggtta ccaggatgat
     1621 ttcgttgttc agaacgcgct gacgcagagc acctttgatt tctgcttcag tcagttcttc
     1681 accacccagg tatttttcca tcagctcttc agaagcttca gctgcggatt cgatcaggtt
     1741 ctggtgccat tcgttagcca gttcaaccat gtctgccggg atatcttcgt attcgaaggt
     1801 tacgccctgg tcagcgtcgt tccagttgat agctttcatt ttcaccaggt caacaacacc
     1861 ggtgaaatgt tcttcagcac caatcgccag ctgcagcgga accgggttcg cgcccagacg
     1921 ggttttgatc tggttaacaa ctttcaggaa gttcgcaccc atgcggtcca ttttgttaac
     1981 gaacgcaatg cgcggaactt tatatttgtt tgcctgacgc catacggttt cagactgcgg
     2041 ctgaacacca ccaactgcgc agtaaaccat taccgcacca tcgagaacac gcatggaacg
     2101 ttctacttcg attgtgaagt caacgtgccc cggggtgtcg atgatgttga tgcgatgcgg
     2161 ctcatactgc ttagccatac cagaccagaa tgcagtagtc gcagcggaag tgatggtaat
     2221 accacgttcc tgctcctgct ccatccagtc catggttgca gcgccgtcat gaacttcacc
     2281 gattttatgg tttacaccgg tgtagaacag aatacgttcg gtagtagtgg ttttaccggc
     2341 gtcgatgtgc gcactgatac cgatgttacg gtagcgtgcg atgggtgttg tacgagccat
     2401 ttgtttcctc gtttatcttt taggcgttca atttaagtag cccaaagcgg gctgcttact
     2461 ggaagcgccc gcctggtgac taaaactccg aagggattac caacggtagt gtgcgaacgc
     2521 cttgttggct tcggccatac ggtgaacgtc ttcacgtttc ttaactgcag tacctttgtt
     2581 ttctgcagca tcagaaagtt cgttcgccag gcgcagagcc atggatttat caccgcgttt
     2641 acgagcagct tcaacgatcc aacgcattgc cagagcatta cgacgaaccg gacggacttc
     2701 aactggtacc tgataagtag aaccaccaac gcggcgagac ttaacttcta cagtcgggcg
     2761 cacgttttcg agagctactt cgaatgcttc cagttcagat ttaccagagc gctgagccag
     2821 ggtctccagc gcgctgtata cgatagattc agcagtagat tttttaccat ctaccatcag
     2881 gatatttaca aatttagcca gcagttctga tccaaacttc ggatccggca gaattttacg
     2941 ctgaccaatg acgcgacgac gtggcatgga aatactccgt tgttaattca ggattgtcca
     3001 aaactctacg agtttagttt gacatttaag ttaaaacgtt tggccttact taacggagaa
     3061 ccattaagcc ttaggacgct tcacgccata cttggaacga gcctgcttac ggtctttaac
     3121 gccggagcag tcaagcgcac cacgtacggt gtggtaacga acacccggga ggtctttaac
     3181 acgaccgcca cggatcagga tcacggagtg ctcctgcagg ttgtgacctt caccaccgat
     3241 gtaggaagtc acttcgaaac cgttagtcag acgaacacgg catactttac gcagcgcgga
     3301 gttcggtttt ttaggagtgg tagtatatac acgagtacat acgccacgtt tttgcgggca
     3361 tgcttccagc gcaggcacgt tgcttttcgc aactttgcga gcacgtggtt tgcgtaccag
     3421 ctggttaact gttgccatta aatagctcct ggttttagct tttgcttcgt aaacacgtaa
     3481 taaaacgtcc tcacacaata tgaggacgcc caattttagg gcgatgccga aaagatgtca
     3541 agaaatatac aacgatcccg ccatcaccag gccatctggc tggagtgctt aaccgtaagt
     3601 ctgacgaaat cagtatagtc aatgggaatg atgtcgttcg aaatttgacc agtcaaaccg
     3661 cgggcaataa ggtcttcgtt cagggcatag accttaatgg gggcattacg cagactttca
     3721 aggtagcggt taccgtcaac tgcggcagtt acgccatctt gcaataatag cagttcgtcg
     3781 ccttcactga gcagacgcag cagcgcagca aaatccgcca gccagggtga acgatgtaat
     3841 gtgtgcagca taaaagcctc aaaacctcaa aataacatcg tagttggcga gttcgcggcg
     3901 taaggcatct gcttcgagcg gcgtggcttc gacaacaaag ggggtctgcg gatctaaccc
     3961 gcgttcgcgc agtgaagccg cacaaaccca gcactgttca atgtcgtaca gacccaacaa
     4021 tttaaaagtg gcaatgtaat cacgcgccag cactgcatcg ggcttttgtc ctggcagcag
     4081 ctgaaaaacg ccatcagcta taaagaagac agccagatca tcagttaatg cggaagttgc
     4141 cagtaaagca tctaaacctt cccgaccagc agctgtacca tgaggcgcag tagaaaaaac
     4201 aaacgcaatt cgtttcatca gaactgtacc accctgtcac aggtcagcga ggcttccgcc
     4261 agcgcgccaa gtccgcttaa ggtaaatccc ggctgaaggt ttgacgaggc cagccccagc
     4321 cttccggcct ccgtttcatc aacaacgcca cggcgtaatg ctgccgctac gcagatattc
     4381 agcgccacac catgttgcgc attcaactgt tgccagccgc gcacaaggtc aaattcgtcg
     4441 cttgccggtg aggtcaattg gttagcgtta tagacccctt cccgataaaa aaagacgctg
     4501 cttaactcat ggccttctac tatcagcgcc tgcgcaaact gaaaagcact actcgcctgt
     4561 tgcgtaccgt atgctggccc ggtcaccacg atggcaaaac gcattactta tcttgcccct
     4621 ggaaatcacc gcttttgaac tggcggatgt agagatagac agtgtgtttg gagatgttca
     4681 ggcggtcagc aacctggttg atcgcgtctt taatatcgaa gatccctttc tcgtagagat
     4741 tcagcacgat ctgacgattt ttggcgttat tagaaacatt gcgatcggca tttacttctt
     4801 cgatggtgaa ctccagcgtt tgggtaacca gatcttcaac agaagaggca aagttgacgc
     4861 ttgaaccgac atccggggtt tctggcggca caaaagtgct cataatctgc gagaagggaa
     4921 catcaagatt catattgatg cacagcagac caattacacg ctgttcgcgg ttacgaatcg
     4981 cgatagtcag ggacttcatt aatacgccgc ttttggcgcg agtaaagtag catttagaaa
     5041 cgctgctatc cgctcccgtc atatcgtgca gcatacgtag cgccaggtca gtaattggcg
     5101 aaccaatctt ccggcctgta tgttcaccgt tagcaatgcg aatggctgaa cattttagat
     5161 cctgcaaaga gtgcaaaacg atttcacagt gggagccaat aagcatcgct aacccgtcca
     5221 ccaccgcttc gtaggatttc agaatatcaa aatcggtctg gtcgaaagga cgttgatcca
     5281 gtaaatccaa ctcactggtt tcgttggtta aaagcgacct ggacatgaaa aaaaacactc
     5341 cttttcagga gcctgtcgtt aacttttcag ggcaggctca ttaatgatgc gggtaactaa
     5401 attaatacag cggaggttcc gctttccagc actaattata tccggcctgt aataaaaaaa
     5461 ccgccgcctg gtcaggcggc ggttcttaat gcttattttt tagcagaatc tgcggctttc
     5521 gcatcagctt ccggctttgc atcagccttc ggcgctggtt tcacatccag cagctctacg
     5581 tcaaacacca gggtagaatt cggtgggatc cccggaacac ccgctttgcc gtaagccagt
     5641 tctggtggaa taaccagttt gatcttaccg cctttcttga tgttcttcag accttctgtc
     5701 caccccggga taacaccgtc cagacggaaa gagagcggtt caccacgggt gtaagagttg
     5761 tcgaactctt taccgtcgat cagcgtacct ttgtagttca ctacaacagt atcgctgtct
     5821 ttcggtgctt cgcctttacc ggcttctact acctgataaa ccagaccagt tgaagaggtt
     5881 ttcacacctt tctctttggc aaatttctcg cggtactctt taccttttgc ttcgttatca
     5941 gccgcgtctt tttccatctt cgcctgagca gaagacttca cgcgagcttc gaatgcttgc
     6001 agagtctgtt cgatctcttg gtcggagagt ttgctcttat cagcaaatgc atcctgaaca
     6061 ccagcgatca gctgatcttt atccagtttg atgcccagtt tttcttgttc tttaagagag
     6121 ttttccatgt aacgacccag cgaagcaccc agtgcataag ctgatttctg atcgtcattt
     6181 ttgaacgctg ctttgctgtc agcagttgta gcaggttttg cagcttcagc agcaaaagtg
     6241 attggtgcat gcagggcaac ggccattgtg gtcgccagca gcgttacttt aaacagtgat
     6301 ttcatccata tctccaggat cggggcatct caccccaggg ttaactatta tcagaagggt
     6361 actataaagc gttgtcgaac aaatctacat acagacacgc cctattatca tctattttca
     6421 gactcttttt gtttaaatta gtttcgatga ccgcgaaatg agtgctgtct cgggcagcaa
     6481 agttaagtag aatccgcggc gaccattcga caaaagaggt gaatcatgca ggatttatca
     6541 ttggaagcac gcctggcaga gctggagagc cgactggctt ttcaggaaat caccattgaa
     6601 gaactgaacg tcacggtgac cgctcatgaa atggagatgg cgaaactgcg cgatcatctg
     6661 cgtctgctga ccgagaagtt aaaagccagc cagccgtcga acatcgcgtc gcaggctgaa
     6721 gaaacgccac cgccacatta ttgaggcgta aaaaaagcgg ggattccccg cttttttgtc
     6781 accttttcgg tattagtggc aaccacaacc gccgttgcct ttaccgccac agcagccttc
     6841 gccaccgtgt tcatgaccgt gatcgtggcc atgaccgccg cagcaaccgt cgtggtcgtg
     6901 atcgtggtga tgatcgtgcg cgccgtgaac gtgaccatga gccagttctt cttcggtcgc
     6961 ttcacgaatc gccacaacct caacgttgaa tttcaggttc tgaccggcca gcatgtggtt
     7021 accatcaacc actacgtgat cgtcttcaac cgcagtgatt tcaaccggta ccggaccctg
     7081 gtcggtttca gccaggaaac gcatacctac ctgcagttca tcaacgccca taaatacgtc
     7141 tttaggaaca cgttgcacca ggttttcgtc gtactgaccg taagcgtcgt tcgcgccaac
     7201 agcgacatca aatttgtcgc caacttcatg accttccagc gccgtttcca ggccagagat
     7261 cagggaaccg tgaccatgca ggtagtccag cggcgcactc accggagact catcaaccaa
     7321 cacaccgtct tctgtacgta cctgataggc caggctgacc accaggtctt ttgctacttt
     7381 catgatatct cctgagcatg ggaagaatag tggcgcagat tgtagcggaa ttctgcagcc
     7441 gtgtactcac tagcttaaaa aaactcggcg catatcgcta gtccggatga aaaatcccga
     7501 tcacttgctc atctttgcga acgtgatcgc gggcttcttt gtctgcttct cgcatctgat
     7561 gtccgcactt aacgcattca acaatatcaa tattattttc gcgccacatc gccattgaat
     7621 cctgtgcctg acaggccggg cattttgcgc ccgcaataaa acgttttcgg attgccatct
     7681 ttaccctcta ctcaaattca tcccagccgt ccagctggcg tcgttcttgt tgcatttcac
     7741 gctggaaaat ctcttccagt tcgcgtcggg cttccctggc gcgagaaatt tgtacagtat
     7801 cggcatgcat tgggatgagc tctcgcagca tccgcatatc caggcggcga aaatgcagtt
     7861 gcgcccgctg cgcctgatgc ggatgcatcc caagcgtgac caaagtcttg cgccccagct
     7921 ctaacgcact ggagaatgtt tcacgggaaa actgcgtcac ccctgcctgt aataactcat
     7981 gcgcttccac acgtccgcgc gctcgcgcaa gaatatgcaa atgcggaaag tgctgctggc
     8041 atatttccac cagcttcatg gtgtcttccg gctcgttaca ggtaatgacg atagactcag
     8101 cggcctctgc acccgcagaa cgtaatagat cgacctgcgt ggcgtcgccg taataaactt
     8161 tgtagccata tttacgcatc aggttaacgg cgctgatatc ccgctccagc acggtaatgc
     8221 gcattttatt tgccatcagc aaacgaccaa tcacctgacc aaaacgcccg aagcccacga
     8281 caatgacctg gggtttatca tcgttgaccc acggtttttc gtcttcttct tccggtccgt
     8341 taaactggcg ggatagccat ttatccacca gcttcatcag caacggcgtg gtcatcatgg
     8401 aaagcgtcac cgtcaccagc aacagcgcca tctggtcgcc ctggaataaa cgttgtgaag
     8461 aagcggtaga aaagaggaca aaggcaaact caccgccctg actcaacacg ccagcaaact
     8521 gcatccgctc tgagctacgc acgccataca atcgcgccag cagatacagc acgagaattt
     8581 tcaccgccac cagcacaacc acgcttatca ctacccacaa cagatgggta taaagcaccc
     8641 cgaggttgag cgacatgccg acagagataa agaacaaacc gagcagcaag cctttgaagg
     8701 gatcgatagc cgtttccagt tcatggcgat attcactttc cgccagcagc acgcccgcaa
     8761 taaatgtacc gagcgccatc gacagcccca gcgcatccat aaacaatgcg gaacccaaca
     8821 ccagcagcag cgtcgcggcg gtgaacactt cccgcacgcc agaagctgca ataaagcgga
     8881 ataccggacg cagtaaatag cgcccaccaa tcagcatgcc gacaaacgcc agcaccttca
     8941 tgccgatctt catccagtcg aaatgttcgt ctgccgaccc cgccaacaac ggcactaacg
     9001 ccagtgctgg gatcactgcc agatcctgaa acagcagaac cgaaaacccg agctggccgg
     9061 attcgctgcg attcatccct ttctcacgca tcaattgcaa cgccattgca gttgaagaca
     9121 tcgcaaggcc aatgccaccg accaccgccg cctgccaggc gaaatccgtc agcatcaata
     9181 atcccgccag caacgccgcg cttaacagca cctgtgccgc gcctacgcca aaaatcgaac
     9241 gccgcagttg ccaaagtttg gagggattca actcaaggcc gatgataaac atcaggaata
     9301 ccacgccgag ttccgaaaag tggaggatct catcgacgtc gctaataaac cccagccccc
     9361 acgggccaat tgcaatccct gccagcaaat atcccaacac agcgccaatc cccagccgcg
     9421 atgccaacgg caccgcagcc accgccgcga agagaaacag cactcctgcg agtaaaaaat
     9481 cggaaccttc catcagcggc ctcctggaga cagcggattt gccagccagt caccgtaggc
     9541 tctggcgtgg ctcgccagct cctttgcgct ttgccgtctc gcccagtaaa taatgatggg
     9601 acttaaccaa tgcatccggc acatgcccgc cgccagttca aaggggcgca gcacatcgct
     9661 catcgggtag cgattcagcg cgtcataacg gtaagcactt tccggctcgc cggtggtaat
     9721 cacgttacgc cagtactttc ccgccagttg gtttcctccc ggcccgctgg caaaaccacg
     9781 acttaatacc cggtccagcc actctttcag tagcgccggg cagctatagg tataaagagg
     9841 atgctgaaag acaatcacct cgtgctcgcg cagtaatgcc tgctcacggg ggatatcaat
     9901 aaaaaaatcg ggatagtgcg cgtaaaggtc gtgcacggta acattgctga gctgcgtggc
     9961 cggtttaagc agtacccggt ttgccaccga gtcctgagat tccggatggg catacagcag
    10021 caaaactttc gctggctgag acatcatccc cctcccggta tggtttttgt gtatagtcgc
    10081 tgttttgggc taccattgcg cccggtgcgg cagctcgccc atacattaca ttatcataat
    10141 gataagttaa catagtctga acatacggcg ccttatgatt gttttctcct cgttacaaat
    10201 tcgtcgcggc gtgcgcgtcc tgctggataa tgccaccgcc accatcaacc ccgggcagaa
    10261 agtcggcctg gtgggtaaaa acggctgtgg taaatctacc ctgctggcat tgctgaaaaa
    10321 tgaaatcagc gccgacggcg gcagctacac ctttccggga agctggcaac tggcgtgggt
    10381 gaatcaggaa acgccagcgt taccgcaagc ggcgctggaa tatgtcattg acggcgaccg
    10441 tgaatatcgt caactggaag cgcagctgca cgacgccaac gaacgtaacg acgggcacgc
    10501 cattgcgacc attcatggca agctggatgc tattgacgca tggagtattc gctcccgtgc
    10561 cgccagcctg ctgcacggcc tcggtttcag caatgaacaa ctggagcgcc cggtaagtga
    10621 tttctccggt ggctggcgta tgcgtcttaa ccttgcccag gcgctgattt gccgttcaga
    10681 cttgctgctg ctcgacgaac cgactaacca cctcgatctc gatgccgtta tctggctgga
    10741 aaaatggttg aagagctatc agggcacgct gatcctgatc tctcacgacc gcgacttcct
    10801 cgatccgatc gttgataaaa ttattcatat cgaacaacaa agcatgttcg agtacaccgg
    10861 caactacagt tcgtttgaag tacagcgcgc cacccgtctg gcgcagcaac aagcgatgta
    10921 tgaaagccag caggaacgcg tggcacatct gcaaagttat atcgaccgtt tccgtgccaa
    10981 agccaccaaa gcgaagcagg cccagagccg cattaagatg cttgagcgta tggagctgat
    11041 tgcccccgcg cacgtcgaca acccgttccg ctttagcttc cgcgcgccgg aaagcctgcc
    11101 aaatccgtta ctgaagatgg aaaaagtcag cgcgggctat ggcgatcgca ttattctcga
    11161 ctcgattaaa ctgaacctgg tgcccggctc gcgcattggt ctgttaggcc gcaacggcgc
    11221 gggtaaatcg acattaatca aactgttagc cggtgaactt gcgccagtca gcggtgaaat
    11281 tggtctggcg aaagggatca agctcggcta cttcgcccag catcaacttg aatacctgcg
    11341 cgccgacgaa tcacctattc aacatctggc acgtttagcg ccgcaggagc tggaacaaaa
    11401 actgcgtgac tacctcggcg gctttggttt ccagggcgat aaagtaaccg aagaaacgcg
    11461 ccgcttctcc ggtggggaaa aagcccgcct ggtgctggca ttaattgtct ggcagcgtcc
    11521 gaatctgctg ctgctcgacg aaccgactaa ccaccttgac ctcgacatgc gtcaggcact
    11581 caccgaagca ttaatcgagt ttgaaggcgc gctggttgtc gtttcgcacg accgtcattt
    11641 gctgcgttcc accactgacg atctctacct ggttcacgat cgtaaagtcg aaccgttcga
    11701 cggcgatctg gaagattatc aacagtggtt gagcgacgta caaaagcagg aaaaccagac
    11761 cgacgaagcg ccaaaagaga acgcgaacag cgcccaggca cgtaaagatc agaagcgtcg
    11821 ggaagcggag ctgcgtgcgc aaacccagcc gctgcgtaaa gagattgccc gtctggaaaa
    11881 agagatggag aagctgaacg cgcaactggc gcaggcggaa gagaaactcg gagacagcga
    11941 actgtatgac cagagccgta aagcggagtt gaccgcctgc ctgcaacagc aagccagcgc
    12001 caaatccggc ctggaagagt gcgaaatggc gtggctggaa gcccaggagc agcttgagca
    12061 gatgctgctg gaaggccaaa gcaactgatg gcgcagataa cgacgaccga tgccaatgaa
    12121 ttcagcagca gtgctgaatt cacccctatg cgcggcttta gcaattgtca tctacaaact
    12181 atgctgccgc gtttgtttcg tcgtcaggtg aaattcaccc cgtactggca gcggctggag
    12241 ttgcccgacg gcgattttgt cgatctcgcg tggagtgaag cccctgcaca ggctcggcat
    12301 aaaccgcgtt tagtggtatt tcacgggctg gaaggcagtc tcaatagccc ttacgcccac
    12361 ggtctggttg aggcggcgca aaagcgcggc tggctgggcg tggtgatgca ttttcgcgga
    12421 tgcagcggtg aaccaaaccg tatgcaccgc atttaccatt cgggcgaaac cgaagacgcc
    12481 agttggtttt tacgctggct gcaacgcgaa tttggtcatg cgccaacggc tgccgtcggc
    12541 tattcgctcg gcggtaatat gctggcctgt ttgctggcaa aggaaggcaa tgatctcccg
    12601 gttgatgcgg cggtgattgt ctctgcgccg tttatgctgg aagcctgtag ctatcatatg
    12661 gaaaagggct tttcccgcgt ttatcagcgt tacttgctga acctgttaaa agccaatgcc
    12721 gcgcgcaagc tggcagccta ccccggaacg ctgccgatta atctcgcgca gttaaaatcg
    12781 gtacgtcgca tccgtgaatt tgacgatcta atcaccgcca gaattcacgg ctacgccgac
    12841 gctatcgact attatcgtca gtgtagcgcc atgccgatgc tgaaccggat cgccaaaccg
    12901 acgctgatta ttcacgccaa agacgatccg tttatggatc atcaggtgat cccgaaaccg
    12961 gaaagtctcc ccccgcaggt ggagtatcaa ctgactgaac atggcggtca tgttggcttt
    13021 attggcggta cattacttca tccgcaaatg tggctggagt cacgcattcc tgactggtta
    13081 acaacgtatc tggaggcgaa atcatgttga ttccgtggca agacctctcc cccgaaacgc
    13141 tggaaaattt aattgaaagc tttgtgttac gtgaaggcac cgattatggt gaacatgagc
    13201 gtacacttga acagaaagtc gccgacgtca aacgccagct acagtgcgga gaagcggtgc
    13261 tggtatggtc ggagctgcac gaaacggtca atatcatgcc gcgcagtcag tttcgcgaat
    13321 agcctgcaac cgtcggcgga cgagataata atgatagcta agtcatggag ttactatgtc
    13381 tgccaaacat ccggtcattg cggtaacagg atccagcggc gcggggacca ccaccaccag
    13441 cctcgcgttt cgtaaaatat tcgcgcagtt aaatctgcat gcagctgagg tggaaggcga
    13501 cagttttcac cgttacaccc gcccggaaat ggacatggcg atccgcaaag cgcgcgacgc
    13561 cgggcggcat atcagctact tcggccccga ggctaacgac ttcggcctgc tggaacaaac
    13621 cttcattgaa tacggtcaga gcggcaaagg gaaatctcgc aaatatctgc atacctacga
    13681 cgaagccgta ccgtggaatc aggtaccggg gacattcacc ccctggcaac ctttaccgga
    13741 acccactgat gtactgtttt atgaaggttt acacggcggc gtagtcacgc cacagcataa
    13801 cgttgcgcag catgtggact tactggtcgg cgtggtgcct atcgttaacc ttgagtggat
    13861 tcaaaaactg atccgcgaca ccagcgagcg cgggcactca cgagaagcag tgatggactc
    13921 agtagtgcgt tcaatggaag actatatcaa ctacatcaca ccgcagtttt cccgcaccca
    13981 tcttaacttc cagcgcgttc ccaccgtcga cacttcaaac ccgttcgcgg caaaaggtat
    14041 cccgtcgctc gatgaaagct ttgtggtgat ccattttcgt aatctggaag ggatcgattt
    14101 cccctggctg ctggcgatgt tgcaaggctc attcatttcc cacatcaata cgttagtggt
    14161 accgggcggc aaaatgggtc tggcaatgga attaattatg ctgccgctgg tgcaacgatt
    14221 gatggaagga aagaaaatcg agtaactctg ctattacgcc ggataaaata ctatccggct
    14281 tcacaacggg atagttaaat cacgcggcaa ccacttcata cgagtgagta atattcaccg
    14341 ctttttccag catcaacgcc actgaacaat atttctcagc agagagatca accgcacgcg
    14401 caaccgctgc gtctttcagg tcgcgaccgg tgacgataaa atgcagatta atgtgcgtaa
    14461 acaggcgtgg tgcctcttcg cggcgttcag aggtcaattt tacttcacaa tcgaccacat
    14521 cctgacgccc tttttgcagg atagaaacca catcgatggc actgcaacca cccgccgcca
    14581 tcaacaccat ttccatcgga cttggtgctt tatcgcctga gttgccgtcc attaaaatct
    14641 ggtgaccaga ggcggattcg cccagaaaag ttaacccttc gacccacttc actcgcgctt
    14701 gcatttttgc tactccactg cgtcaatttt cctgacagag tacgcgtact aaccaaatcg
    14761 cgcaacggaa ggcaacctgg gtcatgctga agcgagacac caggagacac aaagcgaaag
    14821 ctatgctaaa acagtcagga tgctacagta atacattgat gtactgcatg tatgcaaagg
    14881 acgtcacatt accgtgcagt acagttgata gccccttccc aggtagcggg aagcatattt
    14941 cggcaatcca gagacagcgg cgttatctgg ctctggagaa agcttataac agaggataac
    15001 cgcgcatggt gcttggcaaa ccgcaaacag acccgactct cgaatggttc ttgtctcatt
    15061 gccacattca taagtaccca tccaagagca cgcttattca ccagggtgaa aaagcggaaa
    15121 cgctgtacta catcgttaaa ggctctgtgg cagtgctgat caaagacgaa gagggtaaag
    15181 aaatgatcct ctcctatctg aatcagggtg attttattgg cgaactgggc ctgtttgaag
    15241 agggccagga acgtagcgca tgggtacgtg cgaaaaccgc ctgtgaagtg gctgaaattt
    15301 cgtacaaaaa atttcgccaa ttgattcagg taaacccgga cattctgatg cgtctgtctg
    15361 cacagatggc gcgtcgtctg caagtcactt cagagaaagt gggcaacctg gcgttcctcg
    15421 acgtgacggg ccgcattgca cagactctgc tgaatctggc aaaacaacca gacgctatga
    15481 ctcacccgga cggtatgcaa atcaaaatta cccgtcagga aatcggtcag attgtcggct
    15541 gttctcgtga aaccgtggga cgcattctga agatgctgga agatcagaac ctgatctccg
    15601 cacacggtaa aaccatc
//
LOCUS       DAJTZL010000094        15583 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.094, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000094 DAJTZL010000000
VERSION     DAJTZL010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15583
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.094"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..2066
                     /locus_tag="OO544_002554"
     CDS             <1..2066
                     /locus_tag="OO544_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCR6768123.1"
                     /translation="GYVPPENWERGINAFYTSYYVSQYYSDYKASGNSKSTYVRFNSG
                     LNLLGWQLHSDASFSKTNNNPGVWKSNTLYLERGFAQLLGTLRVGDMYTSSDIFDSVR
                     FRGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVTIEQNGFVVYQKEVPPGPFAITDL
                     QLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQPGVSKYDFAAGRSHIEGASKHSD
                     FVQAGYQYGFNNLLTLYGGSMVANNYYAFTLGTGWNTRIGAISVDATKSHSKQDNGDV
                     FDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRTFNDHVWANNKDNYRRDENDVYDI
                     ADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLWRDYWGRSGSSKDYQLSYSNNLRR
                     ISYTLAASQAYDENHHEEKRFNIFISIPFDWGDDVSTPRRQIYMSNSTTFDDQGFASN
                     NTGLSGTVGSRDQFNYGVNLSHQHQGNETTAGANLTWNAPVATVNGSYSQSSTYRQAG
                     ASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDAYVNGQKYRTTNRNGVVVYDGMTP
                     YRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVLVNFDTDQRKPWFIKALRADGQPL
                     TFGYEVNDIHGHNIGVVGQGSQLFIRTNEIPPSVNVAIDKQQGLSCTITFGKEIDESR
                     NYICQ"
     gene            2082..3116
                     /locus_tag="OO544_002555"
     CDS             2082..3116
                     /locus_tag="OO544_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
                     /protein_id="HCR6768124.1"
                     /translation="MEIRIMLFILMMMVMPVSYAACYSELSVQHNLVVQGDFALTQTQ
                     IATYEHNFNDSSCVSTNTITPMSSSDIIVGLYNDTIKLNLHFEWTNKNNITLSNNQTS
                     FTSGYSVTVTPAASNAKVNVSAGGGGSVMINGVATLSSASSSTRGSAAVQFLLCLLGG
                     KSWDACVNSYRNALAQNAGVYSFNLTLSYNPITTTCKPDDLLITLDSIPVSQLPATGN
                     KATINSKQGDIILRCKNLLGQQNQTSRKMQVYLSSSDLLAGSNTILKGAENNGVGFML
                     ESNGSPVTFLNITDNSKGYTNLKEIAAKSKLTDTTVSIPITASYYVYDTNKVKSGALE
                     ATALINVKYD"
     gene            complement(3198..3536)
                     /gene="rcnB"
                     /locus_tag="OO544_002556"
     CDS             complement(3198..3536)
                     /gene="rcnB"
                     /locus_tag="OO544_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HCR6768125.1"
                     /translation="MTIKNKMLLGVLLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(3755..4579)
                     /gene="rcnA"
                     /locus_tag="OO544_002557"
     CDS             complement(3755..4579)
                     /gene="rcnA"
                     /locus_tag="OO544_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HCR6768126.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIIGTA
                     FWMFWRTWRGERNWLENMHEYDYEHHHHDHEDHHDHGHHHHHEHGEYQDAHARAHAND
                     IKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLVVSFSIGLA
                     LTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMGIMR"
     gene            4700..4972
                     /gene="rcnR"
                     /locus_tag="OO544_002558"
                     /pseudo
     CDS             4700..4972
                     /gene="rcnR"
                     /locus_tag="OO544_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
     regulatory      5064..5160
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5195..5983
                     /gene="thiM"
                     /locus_tag="OO544_002559"
     CDS             5195..5983
                     /gene="thiM"
                     /locus_tag="OO544_002559"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HCR6768127.1"
                     /translation="MQVDLLGSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTPWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRAVGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGLGSFVPHFLDALWQLTQEVQA"
     gene            5980..6780
                     /gene="thiD"
                     /locus_tag="OO544_002560"
     CDS             5980..6780
                     /gene="thiD"
                     /locus_tag="OO544_002560"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HCR6768128.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            6845..7663
                     /locus_tag="OO544_002561"
     CDS             6845..7663
                     /locus_tag="OO544_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HCR6768129.1"
                     /translation="MQLRITSRKKLTALLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVAAPVQVRLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKSTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTVEELQGFVDGIKETP"
     gene            7715..8461
                     /locus_tag="OO544_002562"
     CDS             7715..8461
                     /locus_tag="OO544_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR6768130.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(8435..9400)
                     /locus_tag="OO544_002563"
     CDS             complement(8435..9400)
                     /locus_tag="OO544_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCR6768131.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEELQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGEQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(9397..10401)
                     /locus_tag="OO544_002564"
     CDS             complement(9397..10401)
                     /locus_tag="OO544_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HCR6768132.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLEREGKIDPDLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRVSPLGCLLPARDVDSFIDDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRITTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGVNAIDPALKAELDAVNQLDFNRYATALAKYRQQREAV
                     "
     gene            complement(10398..11675)
                     /locus_tag="OO544_002565"
     CDS             complement(10398..11675)
                     /locus_tag="OO544_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6768133.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGALLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQILGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSTDEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            11932..12984
                     /gene="fbaB"
                     /locus_tag="OO544_002566"
     CDS             11932..12984
                     /gene="fbaB"
                     /locus_tag="OO544_002566"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HCR6768134.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGTTIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            13303..14157
                     /gene="gatY"
                     /locus_tag="OO544_002567"
     CDS             13303..14157
                     /gene="gatY"
                     /locus_tag="OO544_002567"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HCR6768135.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTYAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADVFYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPALDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            14186..15443
                     /gene="gatZ"
                     /locus_tag="OO544_002568"
                     /pseudo
     CDS             14186..15443
                     /gene="gatZ"
                     /locus_tag="OO544_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416598.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
     gene            15453..>15583
                     /locus_tag="OO544_002569"
     CDS             15453..>15583
                     /locus_tag="OO544_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="phosphoenolpyruvate-dependent sugar
                     phosphotransferase system; catalyzes the phosphorylation
                     of incoming sugar substrates concomitant with their
                     translocation across the cell membrane; IIB is
                     phosphorylated by IIA and then transfers the phosphoryl
                     group to the sugar; IIC forms the translocation channel;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HCR6768136.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIAR"
BASE COUNT         4088 a         3728 c         3988 g         3779 t
ORIGIN      
        1 gtggctatgt tccaccggaa aactgggagc ggggtattaa tgcgttttat acctcttatt
       61 atgtgagtca gtattacagt gactataaag cgtcgggtaa tagtaagagt acatatgtac
      121 gttttaacag cgggttaaat ttactggggt ggcaactgca ttctgatgcc agtttcagta
      181 aaacaaataa caatccaggg gtgtggaaaa gcaataccct gtatctggaa cgtggatttg
      241 cccaacttct cggcacgctt cgcgtgggtg atatgtacac atcaagcgat atttttgatt
      301 ctgttcgctt cagaggtgtg cggttgtttc gtgatatgca gatgttgcct aactcgaaac
      361 aaaattttac gccacgggtg caggggattg ctcagagtaa cgcgctggta actattgaac
      421 agaatggttt tgtggtttat cagaaagagg ttcctcctgg cccgtttgcg attacagatt
      481 tgcagttggc cggtggtgga gcagatcttg atgtcagcgt gaaagaggcg gacggttcgg
      541 taaccaccta tctggtgcct tatgcagcgg tgccaaatat gctgcaaccc ggcgtgtcga
      601 aatatgattt tgcggcgggt cgtagccata ttgaaggggc gagcaaacat agtgattttg
      661 tccaggcggg ttatcagtat ggttttaata acttattgac gttgtatggt ggctcgatgg
      721 ttgcgaataa ttattacgcg ttcactttgg ggactggctg gaatacacgc attggtgcca
      781 tttccgtcga tgccacgaag tcgcatagta aacaagacaa cggcgatgtg tttgacgggc
      841 aaagttatca aattgcctac aacaaatttg tgagccaaac gtcgacgcgt tttggtctgg
      901 cggcctggcg ttattcgtcg cgtgattacc ggacatttaa cgatcacgtt tgggcaaaca
      961 ataaagataa ttatcgccgt gatgaaaacg atgtctatga cattgccgat tattaccaga
     1021 atgattttgg tcgcaaaaat agcttttccg ccaatatgag ccagtcattg ccagaaggtt
     1081 gggggtctgt gtcattaagt acgttatggc gagattactg ggggcgtagc ggcagtagta
     1141 aggattatca gttgagttat tccaacaacc tgcgacggat aagctatacc ctcgcggcaa
     1201 gccaggctta tgacgagaat catcatgaag agaaacgttt taatattttt atatcgattc
     1261 cctttgattg gggtgatgac gtttcgacgc ctcgtcggca aatatatatg tctaactcaa
     1321 cgacgtttga tgatcagggg tttgcctcaa ataatacggg attatcagga acagtaggga
     1381 gtcgggatca gttcaattat ggtgtcaacc tgagtcatca acatcaggga aatgaaacga
     1441 cagctggagc gaatttaacc tggaacgcgc cggtggcaac agtgaatggc agttatagtc
     1501 agtcgagtac ttatcgacag gctggagcca gtgtttcagg gggcattgtc gcctggtcgg
     1561 gtggcgttaa tctggcgaac cgtctttccg aaacgtttgc tgtgatgaat gcgccaggaa
     1621 ttaaagatgc ttatgtcaat gggcaaaaat atcgcacaac aaaccgtaat ggagtggtgg
     1681 tatacgacgg aatgacacct tatcgggaaa atcacctgat gctggatgtg tcgcaaagcg
     1741 atagcgaagc agaattacgt ggcaaccgga aaattgccgc accttatcgc ggcgcggttg
     1801 tactggttaa ttttgatacc gatcagcgca agccctggtt tataaaagcg ttaagagcgg
     1861 atgggcaacc attaacgttt ggttatgaag tcaatgatat ccacggtcat aatattggcg
     1921 ttgtcggcca gggaagccag ttatttattc gcaccaatga aataccgcca tcggttaatg
     1981 tagcaattga taagcaacaa ggactttcat gcacaatcac cttcggtaaa gagattgatg
     2041 aaagtagaaa ttatatttgc cagtaatagt caggtggttc tatggaaatt cgcataatgc
     2101 tatttatatt aatgatgatg gttatgcctg tgagctatgc ggcatgttat agtgagttat
     2161 ctgttcagca caatttggtt gttcaggggg attttgcact tactcaaaca caaatagcga
     2221 catatgagca taattttaat gattcgtcat gcgtgagtac aaatactatc acccctatga
     2281 gttcgtcgga tattattgtt gggctttata acgataccat aaaattaaat ttacattttg
     2341 aatggaccaa taaaaacaac atcacgttgt caaataatca gaccagtttc accagtggtt
     2401 attcagttac ggtgacacct gcggccagta atgcaaaagt gaatgtttct gcggggggcg
     2461 gcggttcagt gatgattaat ggtgttgcga cattatccag tgcttcatca tcgacacgcg
     2521 ggagtgccgc agtacaattt ctactgtgtt tattaggtgg caagtcatgg gatgcatgtg
     2581 taaatagcta cagaaatgca ttggcacaaa atgcaggtgt ctattccttt aatctgacat
     2641 tgtcatacaa cccgataacc acaacctgca aaccggacga tttattaatt actttagaca
     2701 gtattcccgt ttcacaatta ccagccacag gtaacaaagc aacaataaat agtaaacaag
     2761 gggatattat tctgcgttgt aaaaatttat taggtcaaca aaatcaaaca tcacggaaaa
     2821 tgcaggtgta tttatcaagt tctgatttgt tagccggcag taacacgatc ctgaaaggtg
     2881 cagaaaataa tggagtcgga tttatgcttg aaagcaatgg ttctccagtc acatttttaa
     2941 atatcactga taacagcaaa ggatacacaa atttaaaaga aattgcggca aagtcaaaac
     3001 ttacagatac aacggtttca attccgataa ctgccagtta ctacgtctac gatacaaaca
     3061 aagttaaatc tggcgcactg gaggcaaccg cattaatcaa cgtgaaatac gactaataac
     3121 aagcaagacg agcaagtggc taataataaa aaataacaag gtgtctgcat ttccccctca
     3181 tgatgagggg gcttttttta gcgatgataa aaaatctcac cgtcgtaggc tttaatgatt
     3241 ttaccgtcgg tgtcgctgat caacacgtac gcgccaccca tataggtcca gtgcgtcccg
     3301 gcatcaggcg cgggcaggtt acgtaactgc cactgcttaa tgttgtactc atcggtgcgg
     3361 tacatctctg ggacggtatc acctggtttg aaatgcttaa agtcagcaat gaaactactt
     3421 aactcatact tagaaattcc cgaggtattg gtcgaacccg cggtggctgg tgcggcccag
     3481 gcggcactgg taaccagcaa aagcacaccc agcaacatct tatttttaat ggtcataaac
     3541 tctccacttt ggggatctgt aatatcagtc gcatatcttt cctgccatac aatataactg
     3601 acaagcgata ttttgttagt gatcccatga tttcgtaaaa agtgtaaatg aaaggatgcc
     3661 ctaaaagcat ccttagcgag cagagagtca gcggaaatag ctgatttaag aatcatcctg
     3721 ggctgaagat aacatgcaag cctaaaggat tgagttatcg cattatgccc atgaagccat
     3781 gtacgcccat atacacaccg actaagccaa tcaacagact ggaaaaatag ggggcgcgtt
     3841 tagcgagagt gttaaatccg ctccagcgtt ttgcgacctg ctgaacgctg attgctgcgc
     3901 caacgcctac ggtgacaagc gttaacgcca ggccaatgct gaaactgacg accagtgttg
     3961 cgcccagtgt cagggctttc aactgaatgc atatcaacag cacggtaatt gctgccgggc
     4021 aggggataag gccaccggtt aagccaaata gcaaaatttg ccagttggtg acctctctac
     4081 catcaaagcg tcgtttaatg tcattggcat gggctcgtgc atgggcatcc tgatactcgc
     4141 catgttcgtg atggtgatga tgtccatggt cgtggtgatc ttcgtgatcg tgatgatgat
     4201 gctcataatc atactcgtgc atattctcca gccagttgcg ttcgccgcgc caggtacgcc
     4261 agaacatcca gaacgcggtg ccaataatga tcactgcgga aatcagctgg agccacggtt
     4321 ctgctgattg agcagtaaag cgcttgctga tcaccatccc gccaaaggca attaaccaga
     4381 ccactgcggt atgcgaaata gttgctgcca gtccgagcat caccgcttgt ttaatggtgc
     4441 ctttgatggc gatgataaac gccgccatca tcgtttttga gtgccctggt tccaggccat
     4501 gaagcgcacc aagtaagatg gcgctgggga tgaagaacca ggcgtttcct tgctgaagaa
     4561 gagttgtaaa ttcggtcatg ataataattc ttagtattaa ttcggcaatc tgattctact
     4621 ccccccagta cctgatacta ccccccagta gattaatagt gctatgatat tttcatattc
     4681 ttgttaacca ggtgttgcca tgtctcatac aatccgtgat aaacagaaac tgaaagcgcg
     4741 tgccagtaag attcagggct aggtcgtggc gctcaagaaa atgctcgacg agccgcatga
     4801 atgcgctgca gttttacaac agattactgc tatccgtggc gcggtaaacg gtctgatgcg
     4861 ggaagtgatt aaaggtcatc tgacggaaca catcgttcac cagggggatg agctaaaacg
     4921 tgaagaagat ctggatgtcg ttctgaaggt gctggattca tatatcaaat aatttattaa
     4981 cgcgattgta aaactgccgt ttttcctcgt ttacaacgcg tgcgctggac attaccatcc
     5041 tcctctgcga tttatcatcg caaccaaacg actcggggtg cccttctgcg tgaaggctga
     5101 gaaatacccg tatcacctga tctggataat gccagcgtag ggaagtcacg gaccaccagg
     5161 tcattgcttc ttcacgttat ggcaggagca aactatgcaa gtcgacctgc tgggttcagc
     5221 gcaatctgcg cacgcgttac acctttttca ccaacattcc cctcttgtgc actgcatgac
     5281 caatgatgtg gtgcaaacct ttaccgctaa taccttgctg gcgctcggtg catcgccagc
     5341 gatggttatc gaaaccgaag aggccagtca gtttgcggct atcgccagtg ccttgttgat
     5401 taacgttggc acactgacgc agccacgcgc tcaggcgatg cgtgctgccg ttgagcaagc
     5461 aaaaagctct caaacaccct ggacgcttga tccagtagcg gttggtgcgc tcgattatcg
     5521 ccgccatttt tgtcatgaac ttttatcttt taaaccggca gcgatacgtg gtaatgcttc
     5581 ggaaatcatg gcattagctg gcattgctaa tggtggacgg ggagtggata ccactgacgc
     5641 cgcagctaac gcgatacccg ctgcacaaac actggcacgg gaaactggcg caatcgtcgt
     5701 ggtcactggc gaggtggatt atgttaccga tggccatcgc gccgttggca ttcacggtgg
     5761 cgatccgtta atgaccaaag tggtaggaac tggctgtgca ttatcggcgg ttgtcgctgc
     5821 ctgctgtgcg ttaccaggcg atacgctgga aaatgtcgca tctgcctgtc actggatgaa
     5881 acaagccgga gagcgcgcag tcgccagaag cgaggggcta ggcagttttg ttccacattt
     5941 ccttgatgcg ctctggcaat tgacgcagga ggtgcaggca tgaaacgaat taacgctctg
     6001 acgattgccg gtactgatcc gagtggtggt gcggggattc aggccgatct taaaaccttc
     6061 tcggcacttg gcgcttatgg ttgctcagtt attactgcac tggtggcgca aaatacccgt
     6121 ggcgtacagt cggtgtatcg cattgagcct gattttgtcg ccgcccagct cgattcggtg
     6181 ttcagcgatg tgcgaatcga taccactaaa atcggtatgt tggcggaaac cgatattgtt
     6241 gaagcggtgg cagaacggtt gcaacgttat cagatccaaa acgtggtact cgacaccgtt
     6301 atgctggcaa aaagcggcga cccactgctt tcaccttcgg cagttgccac gctacgcagt
     6361 cgattattgc cacaggtttc attaataacg ccaaacttgc ccgaagcagc cgccttgctc
     6421 gacgcgccac acgcgcgcac cgaacaggaa atgctggaac aagggcgatc gctgttggcg
     6481 atgggctgtg gcgcagtgct aatgaaaggt ggtcatctgg atgatgagca aagcccggac
     6541 tggctgttta cccgcgaggg tgaacaacgg tttaccgcac cgcgcattat gaccaaaaac
     6601 acccacggta ctggttgtac actctctgcg gcgttggctg cactacgccc gcgccataca
     6661 aactgggctg acaccgtaca ggaggcaaaa agctggcttt catcggcgtt agcccaggcc
     6721 gacacgctgg aagttggtca cggtattggt ccggttcacc acttccacgc ctggtggtga
     6781 gcaccgtact atagctgctt tggttattgt cagcctttgg cactgacatt cagggatgaa
     6841 accaatgcag ctaagaatta ccagtcgaaa aaaactcacc gctctattat gcgctcttgg
     6901 gctaatttcc atcgttgcaa tttatccgcg tcagacggtg aattttttct attcgacagc
     6961 agttcagatt acagactaca tccacttcta cggttatcgc ccggttaaat ctttcgctat
     7021 tcgtattcct gccagttaca ccattcacgg aatagatgtt tcgcgctggc aggagcggat
     7081 cgactggcag cgtgtggcaa aaatgcgcga taacggtatt cgcttacaat ttgcctttat
     7141 taaggcgacg gaaggcgaaa agctggtgga cccctatttt tcgcgtaact ggcaactaag
     7201 ccgcgaaaat ggcttgctgc gcggggcata tcattatttc tccccgtcgg tagcagcacc
     7261 ggttcaggtg cgattatttc tgcaaacggt agatttctca caaggcgatt tccctgcggt
     7321 actggacgta gaagagcgag gaaaattatc ggcaaaagaa ttacgcaaga gggtaagtca
     7381 gtggctaaaa atggtcgaaa aaagtacggg gaaaaagccg attatttact caggagccgt
     7441 tttttatcac acgaatctgg cgggctattt caatgaatat ccgtggtggg tggctcacta
     7501 ctatcaacgt cgtccggaca atgacggcat ggcctggcgc ttctggcagc attccgaccg
     7561 tggacaggta gatggcatca atggtccggt ggattttaat gtgtttaatg gcacggtgga
     7621 ggagttgcag ggattcgttg atgggattaa agaaacgcct taaataagaa gcagtgatat
     7681 actcccacca gactccctgg caggaaaaag acaaatggaa caagcgcata cccagctgat
     7741 tgcccaactg aacgaacgta ttttagcggc ggataacacg ccgctttata ttaagtttgc
     7801 cgaaacggta aaaaatgccg tgcgcagcgg ggtgctggag catggcaata ttttgcccgg
     7861 tgagcgtgat ttaagtcagt taaccggcgt ttcgcgcatt acggtgcgca aggcgatgca
     7921 ggcgctggaa gaagagggcg tggtgacgcg ttcgcgcggt tacggcacgc agatcaacaa
     7981 catcttcgaa tactcgttga aagaagcgcg aggtttttct cagcaggtgg tgttgcgcgg
     8041 gaaaaagccc gatacgctat gggttaacaa gcgtgtcgtg aaatgtcccg aagaagtcgc
     8101 gcagcagctg gcggtcgaag caggaagtga tgtctttttg cttaagcgta ttcgctatgt
     8161 cgatgaagaa gcggtatcga ttgaggaatc gtgggttccg gcgcatttaa ttcatgatgt
     8221 tgatgccatc gggatttcac tttatgacta tttccgcagc cagcacattt acccacaacg
     8281 tacgcgttcc cgcgttagcg cccggatgcc ggatgccgag tttcagtcac atattcagtt
     8341 agatagcaaa ataccggtgc tggtgatcaa gcaagtggcg cttgaccaac agcaacggcc
     8401 tattgagtac agcattagcc actgtcgcag cgatctatac gtttttgtgt gcgaggagta
     8461 gttcctcgcg cgttggcgca caatcaccgc cccgatgccc gacaacccac gacgccactg
     8521 cattgcccag taatacggca tccgccagtg gcagaccaga ggccagcccg gcaagtacgc
     8581 cacctgcatg actgtcgccc gccccaatgg tgtctacaac ttgcgttgga aatgccggaa
     8641 tgcagccaga agcgtcgttg ctgaaatacc atgcgccttc tttatcgaga cgaacgatca
     8701 acggcgcggc aaatttctcc tgccattgct cgccaagtgt tgttatctct gcggataaag
     8761 caaaacgttc ggcggcaatc tcagcctctt gacgattgag cgacactaaa ggtcgacagg
     8821 ccatgatccg cgccagtaat gcatccggga tatcgccaat acgtgggcca aaatcgataa
     8881 acggcgtcac gtcttgcagc tcttccagcc attccactaa caattcgccg cagggcgagg
     8941 ccagttgata accggaaaaa tagagcaggc tgccaggcgc aacggttaat cgcgccagcc
     9001 actggcgatt ccactgattt tccacaccgc tgaatgacat aaaagtgcgt tcgccatccg
     9061 gctcaaccag cgccagacac cagccgttat caccttcggc gttatcgatc aggctgatta
     9121 agccctcttt tgccatccgg ttgcgaataa tctccgccca cacgccctga ccgagcggca
     9181 aggcattacc cgcttcgatg ccgaggcgct ttaacgccac ggcaatattc agtgcgcagc
     9241 cgccaacgtt aacgctctgc tgtttcagtt cgatatcgca cccacgccag gggagggcat
     9301 aagcgtcggc gatcacgtca atgaccgccg cgccgacgac catcaccggc tgacgcgtgg
     9361 ttaattcagg cagcagcgtg tgtaatcgag cgccgctcat accgcctccc gttgttgacg
     9421 atatttcgcc agcgctgtgg catagcggtt gaaatcaagc tgatttaccg catccagttc
     9481 cgcctttaac gcaggatcga tagcgttaac gccatgcaac gcgccgcaaa ttgccgtcgc
     9541 catagcaccg atggtgtctg tgtcgccgcc aaggttagcg cacaggacgg cgcagcgatt
     9601 cgggtcggtt tgtgctagtt caaccagcgc aatggcgcac ggaacggact caatagtgct
     9661 ggtacctgcg ccaacgacct ggtaaagctg ttcgctggcg gattcggtgc cgtcggcatt
     9721 gcgcacaatt ttcagcgcaa tctccagccg cgccgccagt gaggcgctga acgtagtgat
     9781 gcgtttttgc tgtgcatgtc gcgcaattga aggcagggaa tcaacaatcg ctgaccagct
     9841 ttctccgtca atggcacgag aaatcgccca tgcgatgact accgcgcctg caaccgccag
     9901 atcggattta tgggtcgggc tggacgccag cgctacatca tcaataaagg aatcaacatc
     9961 acgcgccgga agcaaacaac ctaatggcga gacgcgcatc gctgcgccgt tggtcacgcc
    10021 gttgttttcc agttcagcaa cgggtttacc gtcgcgaatg gcgttaagcg caatcttcga
    10081 ggtcggacct agtacgtttt tgttaaaggc gtcgaaacgc agcgcccagt cgagaatatt
    10141 acgcccaatc agatccggat cgatcttgcc ttcacgttcc agtaacgcat ccgccagaca
    10201 cagcgccatc gaggtatcgt cggtgaattc ggcgcggtta aaataacagg ccgcgttatt
    10261 ctcctttggt ccaggaagaa aacggtcaat ccagccaaag tgtgctttaa cgcggctgcg
    10321 tggccaaagc tcggagggca tccccatcgc atcccctaac gcctgcccat aaagagcacc
    10381 gagaatacgt tctgttttca tttaacttcc ccttgtgtca acgcaatatc gcgatcatcg
    10441 accttgatag ccgtaatttc gttgtcggat tcgcggaaaa aaatcatgaa cagcacggcg
    10501 ataatggcaa tcatcaccgc gccgaaagtc cacatcccgg accagttgaa agtcagtccg
    10561 tttaccggtt cctgataagc gaacatcttt tccatcatca caccgccaag acgatagccg
    10621 agcaaactgc cgaagccctg gcagcagagc gtgatcagcc cctgcgcagc ggtacgcata
    10681 tgcacggggg cttttttatc gacatagatg taagcggtaa cgtagtaaaa atcgtaactt
    10741 acgccgtgaa gcaaaatacc gaggaacagt aacgcgtagg tgaaatattc atccgtacta
    10801 ccgtaaataa agaagccata gcggatcgca gcggtgacca gaccaagcaa taataccttt
    10861 ttgataccaa agcgtttagt gaaaaacggc aatgccagca taaagaagat ttcagagaac
    10921 tggccgagcg tcatccagcc ggtggcgttt ttcatgccaa cttcggtcag ataaccgttg
    10981 gcaaagatgt aatagaacgc cagtggcatc gcaaacagga atgaacagaa gaaaaagacg
    11041 aggaagtttt tatcgcgcag caggatcagc gcatccaggc cgagcatgac tttaatatcc
    11101 attttgccgg tgctttttgg tggcgtgtcg ggcaggaaaa acgcaaacac accgagcaga
    11161 gcagaacttc cggcggtaat cagcagcggg atgttagtcg gtgagatatc ggcatacccc
    11221 agtatttgcg gcaagaaacc acatgccaga ccggaggcaa tccagccgat agtgcccatc
    11281 acacgaatgc gcgggaaatc acgctccaca tccggcacgt tggcaaaagc gatgctgtta
    11341 gtcagcgcaa tggtcggcat ataggttaga gagtaagcca gcagtaacgg aaagaacccg
    11401 gcaaaagtgg tctgttgcgc agcgaaatac atcagcagcg cgcctgcgaa catcaatacc
    11461 gccagcactt tttgcgccga gaaaaagcgg tcagtgatgg agccaaccag aattggcgac
    11521 aggatcgccg caatggcggt acaggcatac gaccagccaa tttctccggc actaaaaccg
    11581 cttttactta accacaacca caatggcaca aaccacgcgc cccagataaa ccattcaaca
    11641 aacatcatga acgacagctt tgctgttgtt ttcatctttt aattccttca tgacaggtaa
    11701 tgtaaggtac gcgatgacaa taccacttaa caataccttt taaatacctt tgcggtgaaa
    11761 acttgaccgc gctaacattt tttctgatga atcgagccaa cagaaaacgc tgaaaaaaca
    11821 tccaaaagat ggaaaaactc gcccgtaacg accaggatta tgattgcccg caaaaggcgg
    11881 gccaggacaa tccatagccg atatccaatc ggaatttacg ggagcatagt aatgacagat
    11941 attgcgcagt tgcttggcaa agacgccgac aaccttttac agcaccgttg tatgacaatt
    12001 ccttctgacc agctttatct ccccggacat gactacgtag accgcgtaat gattgacaat
    12061 aatcgcccgc cagcggtgtt acgtaatatg cagacgttgt acaacaccgg gcgtctggct
    12121 ggcacaggat atctttctat tctgccggtt gaccagggcg ttgagcactc tgccggagct
    12181 tcatttgctg ctaacccgct ctactttgac ccgaaaaaca ttgttgaact ggcgatcgaa
    12241 gcgggctgta actgtgtggc gtcaacttac ggcgtgttgg cgtcggtatc gcggcgctat
    12301 gcgcatcgca ttccatttct cgtcaaactt aatcacaacg agacgctaag ttacccgaat
    12361 acctacgatc aaacgctgta tgccagcgtg gagcaggcct tcaacatggg cgcggtggcg
    12421 gttggtacga ctatctattt tggctcggaa gagtcacgtc gccagattga agaaatttct
    12481 gcggcctttg aacgcgcgca cgagctgggc atggtgaccg tgctgtgggc ctatttgcga
    12541 aactccgcct ttaagaaaga tggcgttgat taccatgttt ccgccgacct gaccggtcag
    12601 gcaaaccatc tggcggcaac catcggtgca gatatcgtca aacaaaaaat ggcggaaaat
    12661 aacggcggct ataaagcaat taattacggt tacaccgacg atcgtgttta tagcaaattg
    12721 accagcgaaa acccgattga tctggtgcgt tatcagttag ctaactgcta tatgggccgg
    12781 gctgggttga taaactccgg cggtgctgcg ggcggtgaaa ctgacctcag tgatgcagtg
    12841 cgtactgcgg ttatcaacaa acgcgctggc ggaatggggc tgattcttgg acgtaaagcg
    12901 ttcaagaaat cgatggctga cggcgtgaaa ctgattaacg ccgtgcagga cgtttatctc
    12961 gatagcaaaa ttactattgc ctgatacgcc tcatacgaac agcaccaccc cgtaggacgg
    13021 ataaggcgtt tacactgcat ccggcaacct atgcctgatg cgacgcggaa gcgtcttatc
    13081 atgcctacat agcactgcca cgtatgtcgg aaaaggcgtt tacaccgcat ccggcataaa
    13141 aacacgcgca ctttgctacg gcttccctat cgggaggccg tttttttgcc tttcactcct
    13201 cgaacagttt tcatattgtc gttttgtgat cgttatctcg acatttaaaa acaaatcatt
    13261 tcattatatt ttgaaatcga aaacaagcaa caggatatga aaatgtacgt ggtatcgaca
    13321 aagcagatgc tgaacaacgc acagcgcggc ggttatgcgg ttccggcatt caatattcac
    13381 aatctcgaaa cgatgcaagt ggtggtggaa accgctgcca acctgcatgc gccggtcatc
    13441 atcgccggaa cgcctggcac attcacttat gctggtacag aaaatctgtt ggcgctggtc
    13501 agcgcgatgg cgaagcaata tcaccatcca ctggcaattc atctcgacca tcacacgaaa
    13561 tttgacgata tcgctcagaa ggttcgttct ggcgtgcgct cagtcatgat tgacgcctcg
    13621 catttgcctt ttgcgcaaaa tatttcacgg gtcaaagagg tggtggattt ttgccatcgc
    13681 tttgatgtca gcgtcgaagc ggagctgggg caacttggcg gccaggaaga tgatgtgcaa
    13741 gtcaatgaag ccgatgtttt ttataccaac cccgctcagg cgcgtgaatt tgccgaggca
    13801 accggaattg attccctggc ggtcgccatc ggcacggctc atgggatgta tgccagcgca
    13861 ccggcgcttg atttttctcg actggagaac attcgccagt gggtgaactt accgctggtg
    13921 ctgcatggcg cgtcagggtt atcgactaag gatattcagc aaaccatcaa actggggata
    13981 tgcaaaatca acgttgcaac ggagctgaaa aatgccttct cgcaggcgtt aaaaaattac
    14041 ctgaccgagc accctgaagc gaccgatccc cgggattatt tgcagtcggc taaatccgca
    14101 atgcgcgatg tggtgagcaa agtgattgcc gattgtggct gcgagggcag ggcataacgc
    14161 acctgccatt taacaaggaa aaaacatgaa aacgttaatt gcccggcata aagctggtga
    14221 acatatcggc atatgttcag tctgttctgc ccatccgttg gttatcgaag cggcgctggc
    14281 atttgatcgc aacagcacgc gcaaagtgct gattgaagca acgtcaaacc aggtcaatca
    14341 atttggcggt tataccggaa tgacaccggc agactttcgc gaatttgttt ttacgattgc
    14401 cgataaagtc ggatttgcac gcgaacgcat tattctcggc ggcgatcatc tggggccaaa
    14461 ctgctggcag caagaaaatg cggatgcggc gatggaaaaa tccgtcgagc tggtaaaagc
    14521 atatgttcgt gctggcttca gtaaaattca tcttgatgcg tcaatgtcct gcgcggggga
    14581 tcccataccg ttagcaccag aaacggttgc ggaacgagct gctttgcttt gctgcggaaa
    14641 gtgtggcgac agattgccag cgtgagcaac tgagctatgt cattggcact gaagttccgg
    14701 ttccgggcgg tgaggccagc gccattcagt cagtacacat cacccatgtt gaagatgccg
    14761 ccaatacttt acgtacgcat caaaaggcct ttattgcccg tgggctgaca gaggcgttaa
    14821 cacgcgtgat tgccatcgtg gtgcagccgg gcgtggaatt tgatcacagc aatattatcc
    14881 attatcagcc gcaggaagcg cagccgctgg cgcaatggat agaaaacacc cgaatggttt
    14941 atgaagcaca ttctaccgat taccagaccc ggacggctta ttgggaatta gtccgcgatc
    15001 actttgcaat attgaaagtc ggtcccgcat taacctttgc tttacgcgag gcgatatttg
    15061 cactggcaca aattgagcag gaacttatcg cccctgaaaa tcgcagcggt tgcctggcgg
    15121 taattgaaga agtgatgctc gacgaaccgc aatactggaa aaaatattat cgtacgggtt
    15181 ttaacgattc attactggat attcgttaca gcctgtcgga tcgtattcgt tattactggc
    15241 cgcatagtcg gattaaaaat agcgtcgaaa cgatgatggt gaatcttgaa ggcgtggaca
    15301 tcccactggg catgattagt cagtatcttc ccaaacaatt tgaacgcatt cagtccgggg
    15361 aattatcagc aataccgcat cagctgatta tggataaaat ttatgatgtt ttgcgcgcct
    15421 atcgctacgg ctgtgcggaa taaggacggt atatgactaa cctgtttgtt cgtagcggaa
    15481 tttcttttgt cgatcgtagc gaagttttaa cccatatcgg taatgagatg ctcgccaaag
    15541 gtgtggttca tgatacctgg ccacaggcat taattgccag aga
//
LOCUS       DAJTZL010000095        15406 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.095, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000095 DAJTZL010000000
VERSION     DAJTZL010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15406
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.095"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            10..453
                     /locus_tag="OO544_002570"
     CDS             10..453
                     /locus_tag="OO544_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004997737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6768137.1"
                     /translation="MDLALVTGQREGDLAAMRWEDIRDGRLYVEQQKTGAKIRISLPT
                     TISRLNLTLADVLDNLKKINGKNEKLLGGKTARTIAAQFRIARDTSGLKWEGDPPPFH
                     EIRSLSGRLHSAEKGGDFTQALLGHRSSSMTDKYRDGRGREWKDI"
     gene            669..758
                     /locus_tag="OO544_002571"
                     /pseudo
     CDS             669..758
                     /locus_tag="OO544_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphatase"
     gene            complement(759..1817)
                     /gene="modC"
                     /locus_tag="OO544_002572"
     CDS             complement(759..1817)
                     /gene="modC"
                     /locus_tag="OO544_002572"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HCR6768138.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(1820..2509)
                     /gene="modB"
                     /locus_tag="OO544_002573"
     CDS             complement(1820..2509)
                     /gene="modB"
                     /locus_tag="OO544_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HCR6768139.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(2509..3282)
                     /gene="modA"
                     /locus_tag="OO544_002574"
     CDS             complement(2509..3282)
                     /gene="modA"
                     /locus_tag="OO544_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6768140.1"
                     /translation="MARKWLNLFAGAALSFAVAGNTLADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTASRQ
                     TLLGNSLVVVAPKASEQKDFIIDSTTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVVSKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(3448..3597)
                     /gene="acrZ"
                     /locus_tag="OO544_002575"
     CDS             complement(3448..3597)
                     /gene="acrZ"
                     /locus_tag="OO544_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HCR6768141.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            3726..4514
                     /gene="modE"
                     /locus_tag="OO544_002576"
     CDS             3726..4514
                     /gene="modE"
                     /locus_tag="OO544_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HCR6768142.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDIQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            4582..6054
                     /gene="modF"
                     /locus_tag="OO544_002577"
     CDS             4582..6054
                     /gene="modF"
                     /locus_tag="OO544_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HCR6768143.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPGEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     ESFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLVTGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            6315..7331
                     /gene="galE"
                     /locus_tag="OO544_002578"
     CDS             6315..7331
                     /gene="galE"
                     /locus_tag="OO544_002578"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308814.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HCR6768144.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            7341..8387
                     /gene="galT"
                     /locus_tag="OO544_002579"
     CDS             7341..8387
                     /gene="galT"
                     /locus_tag="OO544_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HCR6768145.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            8391..9539
                     /gene="galK"
                     /locus_tag="OO544_002580"
     CDS             8391..9539
                     /gene="galK"
                     /locus_tag="OO544_002580"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HCR6768146.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            9533..10573
                     /gene="galM"
                     /locus_tag="OO544_002581"
     CDS             9533..10573
                     /gene="galM"
                     /locus_tag="OO544_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HCR6768147.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKSCPVNMTNHVYFNLDGEQSDVRNHKLQILAGEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            10776..11528
                     /gene="gpmA"
                     /locus_tag="OO544_002582"
     CDS             10776..11528
                     /gene="gpmA"
                     /locus_tag="OO544_002582"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HCR6768148.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(11687..12739)
                     /gene="aroG"
                     /locus_tag="OO544_002583"
     CDS             complement(11687..12739)
                     /gene="aroG"
                     /locus_tag="OO544_002583"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HCR6768149.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPVTENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLDSGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            13055..13435
                     /locus_tag="OO544_002584"
     CDS             13055..13435
                     /locus_tag="OO544_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HCR6768150.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEKHKNTMCKDGRCPD
                     INKKVQIGDGINNDVDTKTDGTTQ"
     gene            13549..14490
                     /gene="zitB"
                     /locus_tag="OO544_002585"
     CDS             13549..14490
                     /gene="zitB"
                     /locus_tag="OO544_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HCR6768151.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSIVVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVITLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(14487..15206)
                     /gene="pnuC"
                     /locus_tag="OO544_002586"
     CDS             complement(14487..15206)
                     /gene="pnuC"
                     /locus_tag="OO544_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HCR6768152.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(15244..>15406)
                     /gene="nadA"
                     /locus_tag="OO544_002587"
     CDS             complement(15244..>15406)
                     /gene="nadA"
                     /locus_tag="OO544_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HCR6768153.1"
                     /translation="CAHCPWMAMNGLQSIAEALEQEGSNHEVLDERLRERALVPLNRM
                     LDFAATLRG"
BASE COUNT         3917 a         4043 c         3850 g         3596 t
ORIGIN      
        1 ggcctttgca tggatcttgc actggtaaca ggacagcgcg aaggagatct ggcggctatg
       61 agatgggagg atataagaga tggcaggcta tacgtggaac aacaaaaaac aggggcaaaa
      121 attcgcattt ctctccccac cacgatttcc agattgaatt taacacttgc cgatgtactg
      181 gataacttga aaaaaataaa cggtaaaaac gaaaagttac tcggagggaa gacagccagg
      241 acaatcgcag cacagttcag gattgcccga gacacatccg gattgaaatg ggaaggtgac
      301 ccaccgccat ttcatgaaat ccgaagttta tccggtcgcc tgcactcagc agaaaaaggc
      361 ggtgatttca ctcaggcgct ccttggacat cgttcatcca gcatgacgga taaataccgt
      421 gacggacgag ggagggaatg gaaagacatt taaaattaat ttcgaatgat ttcgaaacac
      481 cacttgtaat taattgatta taaatgatta attcctaatg ggcatttgct tcatgaatgc
      541 tcctttgcat ttagcaactg actgtaacag ctaaaattag tcgcttttgg cggtgaatgc
      601 tgaattaaaa cgcagaattt tgtccgatag tggtatcggt gtaccattcg cgcagacgaa
      661 aattcttaaa tgacgcggta aaagcgcgcg ccaacattgt gattggtgat aacaccaccg
      721 acagcattgc ccagttcatt tatagccacc tgatttaatc aggcggttat cgacacactt
      781 ttaatttgcg cgtacagcca caggccaggt ttgatcgcca gttcatccct ggcccacggg
      841 ctgatacgcg cccacagcgt tttaccgccg acttccagct ccacttccac ctggccgttg
      901 tcgtcataac tattaacaac ttttgcccgc aatacgttac gaatgctggt ttgctgcggc
      961 ggttgtaaaa ccagagaaac atcggaagcc tgaatgcgga tacgtagcgc agcttgcagc
     1021 ggttcgtcca gcttattgac ccacaaatgc tgatcgccca gcgccagcgc ggtcatcgcg
     1081 taatgcggat gatgctcaag taccgtcact ttcagaatgc tactttgttg ctctttcggc
     1141 agccacggat tcatcacgct actgccccac acttcctcca gcgcgccaaa ggctttcacc
     1201 tgaccgtttt ccagtaccat cactctgtct gccagatgga ggatctcatc cagcgaatgg
     1261 ctgacataca acatcggaat gttgatttcc cgtgtcagcc gttgcagata aggcaacagt
     1321 tcgcgtttac gcggaatatc cagtgacgcc agcggttcat ccagcaacag caattccggt
     1381 gctgtcagca aagcccgacc aatcgccacg cgctgttttt cgcctccgga caggctgcct
     1441 ggtaaacggt caagcaacgg ttcaatgcct aaaagcgcca ccagcttatc gaactgatcg
     1501 accatacttt tcgacatgcc gtagcgcaga ttgccacgca ctttgtaatg cgggaacagc
     1561 cgcgcatcct gaaaaacata gccaacgcga cgcttttccg gcgtcaggca gatacctttt
     1621 tcggcatcat ttagtacccg cccattgagg acaatccgcc ctttttgcgg gcgcgtcagt
     1681 ccactgatgg cgttaatcag cgaagttttt ccggcaccgg agacgccaaa gatagcagtg
     1741 atgccattgg cgggcagtgt ttcattaatg gtcaggcaat ggttgcccaa cgtctgggaa
     1801 aaattcagct ccagcatgat tagcgccccg cccgttcacg gctgattctg gccagccatt
     1861 ctgaaatcaa cagggagatc atcgccagcg caatagaaat aatgcacagt ctcgccgcgc
     1921 cactttcccc gccgggggtc tggatcaggg tatacatggc agagggaatg gttcgcgttt
     1981 cgccaggaat gttcgacaca aaggtgatgg ttgcaccaaa ctcaccgaga gaacgagcaa
     2041 aagccagtac cgtaccaaca ataattcccg gtaaggtcag cggtaacgtg atagtaaaga
     2101 aaacgcgcca gcgcccggca cctagtgttc ttgcggcctg ttcgagtttg acatcaaccc
     2161 cttccagcgc cagacgaatt gcccgcacca tcagcggaaa cgacatgacg gcggcagcga
     2221 gaaccgcgcc gcgccagcta aaggcgaagg taataccaaa ccagtcatac agacgttcac
     2281 cgataaatcc gcgccgtccc atcgaaacta ataataagta accgacgacc acgggcggta
     2341 acaccagcgg tagatgcagt acgctgtcga gcagagcttt gcccggaaac gtgcaacgca
     2401 ccagtaacca ggcaaaaaag atcccaaacg gcaggctaaa cagcacagcc agggaagaaa
     2461 ctttcaggct taataaaact gcctgccatt ctggatcggt cagtatcatt acttggttgt
     2521 aaatccgtaa cgtttaaaga tttccgctgc ctgcggtccc ttcagataat cataaaatgc
     2581 tttcactgtc gcattgttat gcccttccac aacagcaacc ggatattcca cttttttatg
     2641 tgaatcttcc gggaaggtgg caaccacttt tacccctttg ctgacaactg cgtcagaacc
     2701 gtaaacaatg cccaacggcg cttcattgcg ttcgaccaac gccagcgcgc cacgaacatc
     2761 ttccgccgga gccagtttcg gggagagcgt atcccatgcg cccagttttt gcagtgcttc
     2821 ttttgcataa atgccagcag ggacatgttc cggatcgcca accgcgaggc gaccgccatt
     2881 cagcagcgaa gtccagttag ttgtgctgtc gatgatgaaa tctttctgct cgctggcttt
     2941 cggtgctaca acgaccaggc tattgccgag cagtgtctgg cgcgaagccg tatcgatcgc
     3001 ttttttatca accgcataat ccatccattt ctgatcggca gagataaaca gatccgcagg
     3061 cgctcccgct tcaatctgac gggccagagt agatgacgaa gcgaaagaag aaaccacatc
     3121 cacgcctttt tcttttttat actgcgtagc aatgtcctgc attgcgttag tcagtgatgc
     3181 ggcggcgaac accgtgattt tcccttcatc tgccagtgta ttgccagcaa cagcgaaaga
     3241 gagtgccgcc ccggcaaaca agttcaacca tttacgagcc attggtaacc ccttaatgta
     3301 acgttatata ggtgacaatg taacggctag ctcagaattt tcccagcagt tattgctaac
     3361 ctttgtgagg tagataagaa aaaatatcgg ctggaaaagc aggaagttga gaaaaagaaa
     3421 tgcccgacta agcgggcatt cagggaatca atgattttgt ccgggctggt cttttttacc
     3481 aacaccagaa aagatgttga atacttcacc aagaccgtaa atcagaccca ggatgatggc
     3541 catcacgaca ggtaccatga ttacggcgaa taccagactt tttaataact ctaacatggt
     3601 caactccaga tatagtcatg agactattct aaccgctaag cacagaaaag cactcccctt
     3661 ttgtgcggtc agctttgcgt ggcgttcgtt ttccgtcaca ataagacttt tgccaggaca
     3721 ttgttatgca ggccgaaatc cttctcaccc ttaagctcca acaaaaatta ttcgccgacc
     3781 cgcgccggat ttcgctacta aaacacattg cgctttccgg ttccattagc cagggagcga
     3841 aagatgccgg tatcagctat aaaagcgcct gggatgccat taacgagatg aatcagttaa
     3901 gcgagcatat tctggttgag cgcgcaacag gcggtaaagg tggcggcggc gcagtactga
     3961 cccgctatgg tcagcgactg attcagctct atgacttact ggcgcaaatc cagcaaaaag
     4021 cctttgatgt gttaagtgac gatgacgccc tgccgctgaa cagcctgctg gccgcgatct
     4081 cacgtttttc actgcaaacc agcgcccgta accagtggtt cggtaccatc accgcccgcg
     4141 atcatgatga cattcaacag catgttgatg tcttactggc tgacggaaaa acacgcctga
     4201 aagtcgcaat taccgcacaa agcggcgcac gtctggggct ggatgaaggc aaagaagtgt
     4261 tgatattgct aaaagcgccg tgggtaggca ttactcagga cgaagcggtc gcacaaaacg
     4321 ccgacaacca attaccgggt attattagtc atattgagcg cggcgcagag cagtgcgaag
     4381 tattaatggc gctacccgac gggcaaacac tgtgcgccac agtgccggta aatgaagcga
     4441 cttctcttca gcaagggcag aatgtcacgg cctactttaa tgccgacagc gtgattatcg
     4501 ccacgctgtg ctaagcgtgt tgacaatttg ttatgaaaca cgtatccctg tcagtaatcg
     4561 ctgcacaaaa tgggatataa aatgtcatcg ttgcaaattt tgcaaggcac gtttcgtctt
     4621 agcgacacaa aaacgctgca attgcctcag ctaacgttaa acgcgggtga tagttgggcg
     4681 tttgtcggtt cgaatggaag cgggaaatcg gccctggccc gcgcgctggc gggggaactt
     4741 ccgcttttga aaggtgaacg gcaaagccag ttttcccaca tcactcgtct ctccttcgag
     4801 caattgcaaa aactcgtcag cgacgaatgg cagcggaata acaccgatat gctcggccct
     4861 ggcgaagatg acaccggacg cactacggct gaaatcattc aggatgaagt aaaggatgca
     4921 ccgcgttgca tgcaactggc gcagcagttc ggtattaccg ccctcctcga ccgacgcttt
     4981 aaataccttt ccactggcga gacgcgaaaa accctgctgt gtcaggcgct gatgtcggag
     5041 cctgacttgt tgattcttga tgagtcgttc gatggcctgg atgttgcctc acgtcagcag
     5101 ctggctgagc gactcgcctc gttacatcag tccggtatta ctctggtact ggtgctcaat
     5161 cgcttcgatg agatcccgga gtttgtccag tttgctggcg tgctggcgga ttgcacgtta
     5221 gcggaaactg gcgctaaaga ggaactgctc caacaagcac tcgtcgcgca actggcgcat
     5281 agtgaacagc ttgaaggtgt gcaactgccg gagccggatg aaccttcagc acgtcacgcc
     5341 ttacccgcca acgaaccgcg cattgtgctg aacaatggcg tggtttctta taacgatcgc
     5401 cccattctta ataaccttag ctggcaggtg aatccaggtg aacactggca aattgtcggg
     5461 ccaaatggtg caggaaaatc gacgttatta agcctggtta ctggcgatca tccgcaaggt
     5521 tacagcaacg atttgacgct tttcggacga cgtcgcggca gcggcgaaac catctgggat
     5581 atcaaaaagc atatcggtta cgtcagcagt agtttgcatc tggattaccg ggtcagcact
     5641 accgtgcgta atgtgattct ttctggctat tttgattcga ttggcattta tcaggccgtt
     5701 tcggatcgcc agcaaaaact ggtgcagcag tggctggata ttctcggcat tgataaacgt
     5761 acggctgacg ctccgttcca tagtctttcc tggggacagc agcgtctggc gctgatcgtc
     5821 cgcgcactgg tgaaacatcc gacgttgctt attctcgatg aaccactaca ggggcttgat
     5881 ccgctcaatc gccagcttat ccgccgtttt gttgatgtgc tgattagcga aggtgaaacg
     5941 caattgttat ttgtttcgca ccacgctgaa gatgcgcctg cctgtattac ccatcgcctt
     6001 gagttcgtgc cggacggtgg actctatcgc tatgtgctga caaaaatata ttgagtcggt
     6061 agtgctgacc ttgccggagg cggcctaagc gccctctccg gccaacggtt cgacgcatgc
     6121 aggcatgaaa ccgcttcttt tttcagataa aaagcgcaat cattcataaa ccctctgttt
     6181 tataatcact taatcgcgca taaaaaacgg ctaaattctt gtgtaaacga ttccactaat
     6241 ttattccatg tcacactttt cgcatctttg ttatgctatg gttatttcat accataagcc
     6301 taatggagcg aattatgaga gttctggtta ccggtggtag cggttacatt ggaagtcata
     6361 cctgcgtgca attactgcaa aacggtcatg atgtcatcat tcttgataac ctctgtaaca
     6421 gtaagcgcag cgtactgcct gttatcgagc gcttaggcgg caaacatcca acgtttgttg
     6481 aaggcgatat tcgtaacgaa gcgttgatga ccgagatact gcacgatcac gctatcgaca
     6541 ccgtgatcca cttcgccggg ctgaaagccg tcggcgaatc ggtacaaaaa ccgctggaat
     6601 attacgacaa caatgtcaac ggcactctgc gcctgattag cgccatgcgc gccgctaacg
     6661 tcaaaaactt tatttttagc tcctccgcca ccgtttatgg cgatcagccc aaaattccat
     6721 acgttgaaag cttcccgacc ggcacaccgc aaagccctta tggcaaaagc aaattgatgg
     6781 tggaacagat cctcaccgac ctgcaaaaag cccagccgga ctggagcatt gccctgctgc
     6841 gctacttcaa cccggttggc gcgcatccgt cgggcgatat gggcgaagat ccgcaaggca
     6901 ttccgaataa cctgatgcca tacatcgccc aggttgctgt aggccgtcgc gactcgctgg
     6961 cgatttttgg taacgattat ccgaccgaag atggtactgg cgtacgcgat tacatccacg
     7021 taatggatct ggcggacggt cacgtcgtgg cgatggaaaa actggcgaac aagccaggcg
     7081 tacacatcta caacctcggt gctggcgtag gcagcagcgt gctggacgtg gttaatgcct
     7141 tcagcaaagc ctgcggcaaa ccggttaact atcattttgc accgcgtcgc gaaggcgacc
     7201 ttccggccta ctgggcggac gccagcaaag ccgaccgtga actgaactgg cgcgtaacgc
     7261 gcacactcga tgaaatggcg caggacacct ggcactggca gtcacgccat ccacagggat
     7321 atcccgatta aggaacgacc atgacgcaat ttaatcccgt tgatcatcca catcgccgct
     7381 acaacccgct caccgggcaa tggattctgg tttcaccgca ccgcgctaag cgcccctggc
     7441 agggggcgca ggaaacgcca gccaaacagg tgttacctgc gcacgatcca gattgcttcc
     7501 tctgcgcagg taatgtgcgg gtgacaggcg ataaaaaccc cgattacacc gggacttacg
     7561 ttttcactaa tgactttgcg gccttgatgt ctgacacgcc agatgcgcca gaaagcaacg
     7621 atcctttgat gcgttgccag agcgcgcgcg gtaccagccg ggtgatctgc ttttcaccgg
     7681 atcacagtaa aacgttgcca gaactgagcg ttgcggcatt gacggaaatc gtcaaaacct
     7741 ggcaggagca aaccgcagag ctgggaaaaa cgtacccgtg ggtgcaggtc tttgaaaaca
     7801 aaggtgcggc gatgggctgc tctaacccgc atccgcacgg acagatttgg gcaaatagct
     7861 tcctgcctaa cgaagctgag cgcgaagacc gcctgcaaaa agaatatttt gccgaacaga
     7921 aatcaccaat gctggtggat tatgttcagc gcgagctggc agacggtagc cgtaccgttg
     7981 tcgaaaccga acactggtta gccgttgtgc cttactgggc tgcctggccg ttcgaaacgc
     8041 tactgctgcc caaagcccac gttttgcgga tcaccgattt gaccgacgcc cagcgcagcg
     8101 atttggcgct ggcgttgaaa aagctgacca gtcgttatga caacctcttc cagtgctcct
     8161 tcccctactc tatgggctgg cacggcgcac catttaatgg cgaagagaat caacactggc
     8221 agctgcacgc gcacttttat ccgcctctgc tgcgctccgc caccgtacgt aaatttatgg
     8281 ttggttatga aatgctggca gagacccagc gagacctgac cgcagaacag gcagcagagc
     8341 gtttgcgcgc agtcagcgat atccattttc gcgaatccgg agtgtaagaa atgagtctga
     8401 aagaaaaaac acaatctctg tttgccaacg catttggcta ccctgccact cacaccattc
     8461 aggcgcctgg ccgcgtgaat ttgattggtg aacacaccga ctacaacgac ggtttcgttc
     8521 tgccctgcgc gattgattat caaaccgtga tcagctgtgc accacgcgat gaccgtaaag
     8581 ttcgcgtgat ggcagccgat tatgaaaatc agctcgacga gttttccctc aatgcgccca
     8641 ttgtcgcgca tgaaaactat caatgggcga actacgttcg tggcgtggtg aaacatctgc
     8701 aactgcgtaa caacagcttc ggcggtgtgg acatggtgat cagcggcaat gtgccgcagg
     8761 gtgccgggtt aagttcttcc gcttcactgg aagtcgcggt cggaaccgta ttgcagcagc
     8821 tttatcatct gccgctggac ggcgcacaaa tcgcgcttaa cggtcaggaa gcagaaaacc
     8881 agtttgttgg ctgtaactgc gggatcatgg atcagctaat ttccgcactc ggcaagaaag
     8941 atcatgcctt gctgattgac tgccgctcac tggggactaa agcagtttcc atgccgaaag
     9001 gtgtggctgt cgtcatcatc aacagtaact tcaaacgtac cctggttggc agcgaataca
     9061 acacccgtcg tgaacagtgc gaaaccggtg cgcgtttctt ccagcagcca gctctgcgcg
     9121 atgtcaccat tgaagagttc aacgctgttg cacatgagct ggacccaatc gtggcgaaac
     9181 gcgtgcgtca tatcctgact gaaaacgccc gcaccgttga agctgccagc gcgctggagc
     9241 aaggcgatct gaaacgtatg ggcgagttga tggcggagtc tcatgcctct atgcgcgatg
     9301 atttcgaaat caccgtgccg caaattgaca ctctggtaga aatcgtcaaa gcagtgattg
     9361 gcgacaaagg tggcgtacgc atgaccggcg gcggatttgg cggctgtatc gtcgcgctga
     9421 tcccggaaga gctggtgcct gccgtacagc aagctgtcgc tgaacaatat gaagcaaaaa
     9481 caggtattaa agagactttt tacgtttgta aaccatcaca aggagcagga cagtgctgaa
     9541 cgaaactccc gcactggcac ccgatggtca gccgtaccga ctgttaactt tgcgtaacaa
     9601 cgcagggatg gtagtcacgc tgatggactg gggtgcgact ttactttcag ctcgtattcc
     9661 gctttccgat ggcagcgtcc gcgaggcgct gctcggctgt gccagcccgg aatgctatca
     9721 ggatcaggcc gcgtttctgg gggcctctat tggtcgttat gccaaccgca tcgccaatag
     9781 ccgttatacc tttgacggtg aaacggtgac gctttcgcca agtcagggcg ttaaccagct
     9841 gcacggcggc ccggaagggt tcgacaaacg tcgctggcag attgtgaacc agaacgatcg
     9901 tcaggtgctg tttgccctga gttcagatga tggcgatcag ggcttcccgg gtaatctcgg
     9961 cgcgacggtg caatatcgtc tgaccgacga taaccgtatc tccattactt atcgcgccac
    10021 agttgataaa tcttgcccgg tgaatatgac taatcacgtc tatttcaatc ttgacggcga
    10081 gcagtctgac gtgcgcaatc acaagttgca gattctggcg ggcgaatatc tgccggttga
    10141 tgaaggcggc attccgcacg acggcctgaa atctgtcgcc ggaacctctt ttgatttccg
    10201 cagcgccaaa atcatcgcca gtgagtttct tgccgacgac gatcagcgca aagtgaaagg
    10261 ttacgatcac gcattcttgt tacaggccaa aggcgatggc aagaaagtgg cggcgcatgt
    10321 ctggtcagca gatgaaaaat tgcagctgaa ggtctacacc accgctcccg ctcttcaatt
    10381 ctactccggc aacttcctcg gcggcacacc gtcgcgggga accgaacctt acgccgactg
    10441 gcaaggactg gcgttggaaa gcgagtttct gccggacagc ccgaaccacc ctgaatggcc
    10501 gcaaccggac tgcttcctgc gtcctggcga agagtattcc agcctgacgg aatatcagtt
    10561 tattgctgag taatgttaag ccctcgtcat gagggcttta tcttcatatt gttcaaatca
    10621 ccagcaaaca ccgacatatt tgcaactcaa tattcacaac aaccttacac tgcgccacta
    10681 ttttcgctat ggttatgcgt aagcattgct gttgcttcgt cacggcaata taatgagaat
    10741 tattatcata aaaagataat ttgaggagta agtatatggc tgtaactaag ctggttctgg
    10801 ttcgtcatgg cgaaagtcag tggaacaaag aaaaccgttt caccggttgg tacgacgtgg
    10861 atctgtctga gaaaggcgta agcgaagcaa aagcagcagg taagctgctg aaagaggaag
    10921 gttacagctt tgactttgct tacacttctg tgctgaaacg cgctatccat accctgtgga
    10981 atgtgctgga cgaactggat caggcatggc tgcccgttga gaaatcctgg aaactgaacg
    11041 aacgtcacta cggtgcgttg cagggtctga acaaagcgga aaccgccgaa aagtatggcg
    11101 acgagcaggt gaaacaatgg cgtcgtggtt ttgccgtgac tccgccggaa ctgactaaag
    11161 atgatgagcg ttatccgggt cacgatccgc gttacgcgaa actgagcgag aaagagctgc
    11221 cgctgactga aagcctggcg ctgaccattg accgcgtgat cccttactgg aatgaaacta
    11281 ttctaccgcg tatgaagagc ggtgagcgcg tgatcatcgc tgcacacggt aactctttac
    11341 gtgcgctggt gaaatatctt gataacatga gtgaagaaga gattcttgag ctgaatatcc
    11401 cgaccggcgt gccgctggtg tatgagttcg acgagaattt caaaccgctg aaacgctatt
    11461 atctgggtaa tgctgacgag atcgcagcga aagcagcggc ggttgctaac cagggtaaag
    11521 cgaagtaaac gtcattcgtt taaaaatgag aaagccgact gcaagtgagt cggctttttt
    11581 gttgctaaca atggagcaca ttgcctgatg cgacgctgcg cgtcttatca ggcctgtggt
    11641 gattcatcgg atacgccact ctgacggcgc atccgacaat taaaccttac ccgcgacgcg
    11701 cttttactgc attcgccagt tgacgtaaca gagcatcggt atcttcccag ccaatgcagg
    11761 catcggtgat gctcttaccg taggccagcg gctccccgct atcgaggctc tgattgcctt
    11821 ccaccagatg gctttccacc atcacgccaa taatggcctt ttcgccaccg gcaatctgct
    11881 ggcaaacgtc agcacaaaca tccatctgct ttttgaattg tttggacgag ttagcatggc
    11941 tgaaatcgat catcacctgt gctggcaggc ctgctttgtt cagcccttct ttcacttcag
    12001 caacgtgctt cgcgctgtag ttaggctctt taccgccgcg cagaatgata tggcaatcac
    12061 cgttaccgct ggtattcaca atcgccgaat gcccccattt agttacggac aggaagcagt
    12121 gcggcgcacc ggcggcatta atggcatcga tagccacttt aatcgtaccg tctgtgccat
    12181 ttttaaaacc taccggacaa gaaagaccag acgccagttc gcggtgcacc tgcgattccg
    12241 tggtacgtgc accaattgcg ccccagctca tcaggtcagc gagatactga ggagtgatca
    12301 tatcgagaaa ctcacccgcc gctggcagac cgctgtcgtt aatatcaagc agcaatttac
    12361 gggctatacg cagaccgtcg ttgatctgga agctgttatc catatgcgga tcgttaatca
    12421 accctttcca gcccaccgtg gtacgcggct tttcaaaata gacgcgcatt acgatttcca
    12481 gctcatcttt cagctcttca cgcagcgcca gcaagcgagt ggcatactct ttagccgcga
    12541 caggatcatg aattgagcac gggccaatca ccaccaacag gcgatcatca ttacctttca
    12601 ggatcttatg gatcgctttt cgggcatggg caaccgtatt cgcggcattt tcagttacgg
    12661 ggaatttttc cagcaatgcg acaggaggaa gtaactcttt gatttctttg atgcgtaaat
    12721 cgtcgttctg ataattcatg tctgttccag tgttgccata cttatcttag tgaatgcaat
    12781 acttccaatc tatatcttcc gtcagaatgt gtaaacgggg ttttacacta tgaacggatt
    12841 aatcctggaa ttagcaagaa aaacgccaga ttgtcgcgaa aaacgagatc tctcctacaa
    12901 tttctaactg taactccttt gtttgttaat tatttcaaga ttctctgctg cgtttcataa
    12961 actggctgaa atcttaaacc aatgccttat attcacctgc aaatgcactg ttggaagagg
    13021 ttatccgaca taacgaccat aacaggagca tcctatgaaa atgacaaaac tggccacatt
    13081 atttctgact gccactctaa gccttgccag cggtgccgca ctggctgccg atagcggagc
    13141 gcaaactaat aacggccagg caaacgccgc agctgatgcg ggccaggtag cccctgacgc
    13201 ccgtgaaaat gtcgcgccaa ataacgtcga caataacggg gtaaataccg gttctggcgg
    13261 cacaatgctg cattcggatg gttcttcaat gaacaatgac ggaatgacca aagatgaaaa
    13321 gcacaagaac accatgtgca aagatggtcg atgtccggat atcaataaaa aggtacaaat
    13381 cggtgatggc atcaataatg atgtcgatac caaaaccgac ggtaccacgc agtaaccctg
    13441 caaaatagcc aataatggga gagcggagtc tctccctttt aatttttcac ttctaaaagc
    13501 agtatgatgt cataaagtta agtgagaata attaagggat gactcgtcat ggcgcactca
    13561 cactcacaca cgtcctcaca cctgccagaa gataataatg ctcgtcgctt gttgtatgct
    13621 ttcggcgtga ctgccgggtt tatgctggta gaagtcgttg gtggttttct ttctggttct
    13681 ctggcattgc tggccgatgc gggtcatatg ttgaccgata ctgccgccct gcttttcgcc
    13741 ctactcgccg tgcaattttc acgtcgtcct cccaccattc gccacacttt cggttggcta
    13801 agactgacca cgctcgcggc gtttgtgaat gccatcgccc tcgtggtaat taccattttg
    13861 attgtctggg aggcgataga acgcttccgc acgccgcgtc cggtcgaggg cggcatgatg
    13921 atggcaattg ccgtggctgg gttgctggca aatatacttt ctttctggtt acttcatcac
    13981 ggcagcgaag agaaaaacct caacgtacga gcagcggcat tgcatgtgct cggggacctg
    14041 ttgggttcgg ttggggctat tattgccgcg ttgattatta tctggaccgg ctggacacct
    14101 gctgacccca ttctctcgat agtggtgtcg cttctggttc tgcgtagcgc gtggcgactg
    14161 ttgaaagata gcgtgaatga attacttgaa ggtgcaccgg tatcgctgga tatcgctgaa
    14221 ctgaagcgtc gtatgtgccg ggaaatcccg gaagtccgca atgtgcacca tgtacatgta
    14281 tggatggtag gcgagaagcc ggtgataacg ctgcatgtgc aggtgatccc gccacacgat
    14341 cacgatgcct tgttggatca gatccaacac tacctgatgg atcactatca gattgagcac
    14401 gccaccattc agatggaata tcaaccttgt catgggccag actgccacct gaatgaaggg
    14461 gtgtctggcc attcacatca tcaccattaa tgggacagcg cgcgtgagcc tctttcacgt
    14521 gcgctgttga tccacatccg actgccgttg agcgcaataa aggtcaggat gatgtactcc
    14581 agagacatgg cgtaaacgcc ctgaagtgca aaaataacga cgctaatcac gttaataatc
    14641 acccacaaca gccagttttc cacatactta cgcgtcatca gaatcattgc cacgatagat
    14701 aacaccatca tgcatgaatc ccagaacggg aaagcgtccg gttgcagttc aggcatcaca
    14761 acctgtaatc ctaatgcttg catgatcatg actgccacgc gggtcaaaaa tgcaaacacc
    14821 ggattgataa ataccgtcat cagaccaatc gaaacaacgc aaaccgccaa ccagctgagc
    14881 gccttcggca atggtaacca gcgaattttc aactccgcct cgttctgact ggtttgtcgc
    14941 gaccacgcat accaaccgta aatattcgcg gcaaagaaaa acacctgtaa tagcaggctg
    15001 gcatacagct gaatctgaaa gaaaataatg ccaaacaagg tgacgttgat caggccaaag
    15061 aagtagttgc tgatcttctc cagactggca aggccaatac acagcaaccc ggcgatcgtg
    15121 cctaccgctt cgatccatga gagatcataa ccgcctgccc ctattggtat atgtaccagg
    15181 atattctgca cactaaaaaa atccatcttt cccccaaagc gtaacgttac gccttattat
    15241 tcgttatcca cgtagtgtag ccgcaaaatc cagcatacga ttgagcggca ccagcgccct
    15301 ctctcgcagc ctttcatcaa gaacctcgtg attgcttcct tcctgttcta atgcctctgc
    15361 gatggactga aggccattca tggccatcca cggacaatgc gcgcag
//
LOCUS       DAJTZL010000096        15247 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.096, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000096 DAJTZL010000000
VERSION     DAJTZL010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15247
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.096"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..941
                     /locus_tag="OO544_002588"
     CDS             <1..941
                     /locus_tag="OO544_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR6768154.1"
                     /translation="MLGEKRLVSSVTKEDLLFVRRDLLTGYQKLSNGKISSIKGRSVV
                     TVNYYMTTIAGMFQFATDNGYTSGNPFNGLTPLKKSKIEPDPLTRDEFIRFIEACRHQ
                     QTKNLWIIAVYTGIRHGELVSLAWEDIDLKARTITIRRNYTKLGEFTPPKTDAGTGRT
                     IHLVQPAIDALKSQAEMTMLGKQHSVEVKQREYGRSTVHKCTFVFSPQVIKQRQFSGP
                     HYKVDSIRESWTSILKRAGLRHRKSYQSRHTYACWSLAAGANPSFIASQMGHTNAQMV
                     FNVYGAWMKDNNHEQIELLNKRLSESVPCMPHKKVG"
     gene            977..1663
                     /gene="mlrA"
                     /locus_tag="OO544_002589"
     CDS             977..1663
                     /gene="mlrA"
                     /locus_tag="OO544_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HCR6768155.1"
                     /translation="MPVTLRAWQRRYGLLKPQRTDGGHRLFNDADIDRIREIKRWIDN
                     GVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRTWIKERGQDYPAQTLT
                     THLFIPLRRRLQCQQPTLQALLAILDGVLINYIAICLASARKKQGKDALVVGWNIQDT
                     TRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGENRTSAQQQQLTSWQE
                     QGHDIFPLGI"
     gene            1723..1830
                     /locus_tag="OO544_002590"
     CDS             1723..1830
                     /locus_tag="OO544_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HCR6768156.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(1811..2542)
                     /gene="yehW"
                     /locus_tag="OO544_002591"
     CDS             complement(1811..2542)
                     /gene="yehW"
                     /locus_tag="OO544_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HCR6768157.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(2547..3473)
                     /gene="yehX"
                     /locus_tag="OO544_002592"
     CDS             complement(2547..3473)
                     /gene="yehX"
                     /locus_tag="OO544_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HCR6768158.1"
                     /translation="MIEFGHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVRLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLEEA"
     gene            complement(3466..4623)
                     /gene="yehY"
                     /locus_tag="OO544_002593"
     CDS             complement(3466..4623)
                     /gene="yehY"
                     /locus_tag="OO544_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HCR6768159.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGVGCAWLTACFIPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGTLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            complement(4630..5547)
                     /gene="osmF"
                     /locus_tag="OO544_002594"
     CDS             complement(4630..5547)
                     /gene="osmF"
                     /locus_tag="OO544_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HCR6768160.1"
                     /translation="MPLSKVWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAQ
                     QGYEKVKKLDAEQNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLKEGGTF
                     KLAASAEFIERADALPAFEKAYDFKLDQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPKGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWTK"
     gene            complement(5799..8096)
                     /gene="bglX"
                     /locus_tag="OO544_002595"
     CDS             complement(5799..8096)
                     /gene="bglX"
                     /locus_tag="OO544_002595"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HCR6768161.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSTMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMEELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNAV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLITALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            8292..10007
                     /gene="dld"
                     /locus_tag="OO544_002596"
     CDS             8292..10007
                     /gene="dld"
                     /locus_tag="OO544_002596"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCR6768162.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYIHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(10046..10978)
                     /gene="pbpG"
                     /locus_tag="OO544_002597"
     CDS             complement(10046..10978)
                     /gene="pbpG"
                     /locus_tag="OO544_002597"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HCR6768163.1"
                     /translation="MPKFRVSLFSLALMLAVPLAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(11152..11739)
                     /locus_tag="OO544_002598"
     CDS             complement(11152..11739)
                     /locus_tag="OO544_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HCR6768164.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            11909..12487
                     /locus_tag="OO544_002599"
     CDS             11909..12487
                     /locus_tag="OO544_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR6768165.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDHQA"
     gene            complement(12617..13378)
                     /locus_tag="OO544_002600"
     CDS             complement(12617..13378)
                     /locus_tag="OO544_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR6768166.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLELGKLPEGAQALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKTMALELVRHKILVNAVAPGAIATPMNGMDDSDVKPDAEPSIPLRRFGATHEIA
                     SLVAWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(13431..14867)
                     /gene="mdtQ"
                     /locus_tag="OO544_002601"
     CDS             complement(13431..14867)
                     /gene="mdtQ"
                     /locus_tag="OO544_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HCR6768167.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAVATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVEGVETDINASKTRQQLNDVAGKMKIIEARLSALTNNQTKSLKLKP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLNIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIVGSRVSEARIPALRE
                     RANGLLLQGQWLDASIQLTGALGGGYKR"
     gene            15091..15174
                     /gene="yohP"
                     /locus_tag="OO544_002602"
     CDS             15091..15174
                     /gene="yohP"
                     /locus_tag="OO544_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohP"
                     /protein_id="HCR6768168.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
BASE COUNT         3713 a         4156 c         3820 g         3558 t
ORIGIN      
        1 caatgttagg tgaaaaaaga ctggtttcat ctgtaacaaa agaggatttg cttttcgtaa
       61 ggagagatct gttgaccggt tatcaaaagc tttctaacgg aaaaatttct tccataaaag
      121 gacgctcagt ggtcacagta aactactata tgacaaccat agctggaatg tttcaatttg
      181 caacagataa tggttataca tcaggaaatc catttaacgg tctgacacca ttaaaaaagt
      241 ccaagataga accagatccc ctcacccgtg acgaatttat tcgttttatt gaggcttgcc
      301 gtcatcaaca aacaaaaaac ctgtggatta tcgctgtata cacgggtatt cgtcacgggg
      361 agctggtatc actggcctgg gaagatatag accttaaagc gaggactata accatccgcc
      421 ggaattatac aaaactaggc gaattcactc caccaaaaac tgatgctggt accggaagaa
      481 cgattcatct agttcaacca gctattgatg ctcttaaaag ccaagcggaa atgaccatgc
      541 ttggaaaaca gcattctgta gaggtaaagc agagggaata tgggagaagt actgtgcata
      601 aatgcacttt tgtttttagt cctcaggtaa taaaacagcg gcagttttcc ggaccgcact
      661 ataaggttga ctccatcagg gagtcatgga caagtatctt aaaacgcgca ggtctgagac
      721 acagaaaatc gtaccaatcc aggcatactt atgcatgctg gtcacttgcc gctggagcta
      781 atcctagttt tatcgcaagc cagatgggcc acacaaacgc acaaatggta ttcaatgttt
      841 acggagcatg gatgaaagac aacaatcacg aacagataga actccttaac aaaagactat
      901 ctgaaagtgt cccatgtatg ccccataaga aagtggggta aaataaaaac ttgtaaaatc
      961 agttagttta cccttaatcc ctgtcacgtt acgcgcgtgg cagaggcgtt acgggttgct
     1021 gaaaccgcaa cggacagacg gcggtcatcg actgttcaac gatgccgata ttgaccggat
     1081 ccgcgagatc aaacgctgga tcgacaacgg cgtgcaggtc agcaaagtta aaatgctgct
     1141 cagtaatgaa aatgttgatg tgcagaacgg ctggcgcgat cagcaagaaa cattactgac
     1201 ctacctgcaa agcggcaatc tgcatagcct gcgaacgtgg atcaaagagc gcggtcagga
     1261 ttaccccgcc cagacactca ccacacatct gtttattcct ctgcgccgac ggcttcagtg
     1321 ccaacaaccg actctccagg cgctgctggc gatcctcgac ggcgtactga tcaactacat
     1381 cgccatttgt ctggcttcgg cacgtaaaaa acagggtaaa gatgcgctgg tggttggctg
     1441 gaatattcag gataccaccc gtctgtggct ggagggctgg attgccagtc aacaaggatg
     1501 gcgcattgat gtcctcgccc actcgctcaa tcaactacgc cctgaactat tcgaaggccg
     1561 tacattgctg gtgtggtgcg gtgaaaatcg aacctccgcc caacagcagc aactcaccag
     1621 ttggcaagaa caaggccatg atattttccc actcggcatt taatgattcg ttaacaaatg
     1681 cgctttactg tacaatcctt tcgttaacat aaggagtgca atatgcgcat agctaaaatt
     1741 ggggtcatcg ccctgttcct gtttatggcg ttaggcggaa ttggtggcgt catgctcgca
     1801 ggttatacct ttattttgcg tgctggctaa gcgcctgcac cagcctttca aacaggcggt
     1861 ctgcgatgat cgccgccagt gccaccagta acgccccctg gatcacatac gcggtattaa
     1921 atccgctaag cccgatgatg atgggcgtac ccagcgtgct ggcccctacc gttgaggcga
     1981 tcgtcgccgt accaatgttg ataatcaccg aagttcgcac gcccgccaga atcaccggag
     2041 ccgccagcgg tagctcgacc ttacgcagtc gctgaccacg actcattccc atacctttcg
     2101 caacttctgt cacgctggca tcaatcgctc ccagcccggc aagtgtcgcc tgcaggacgg
     2161 gcagcacacc gtaaaggatc aaggcgataa tcgctggttg cagaccaaag ccgatcaccg
     2221 gaacggcaat cgccagcact gcgacaggcg gaaaagtctg tccaacggcg gcaatagttt
     2281 ccaccagtgg gcgaaattcc gcgccccacg ggcgagtgac agcaattccg gcaccagtgc
     2341 caatgatcac cgcaaacaaa ctcgaaattc ccaccagcca gaaatgagcc agtgccagag
     2401 ctgcaaaact ttcttgctga taaacgggtc gtggcagttg tgggaacaag gcagcaaaca
     2461 gcggctggct gtaaggcagc cagaaaatca gtgccacaaa caaagcaatg agccagaaca
     2521 gcggatcgcg caacatcttc atacgcttac gcctcctcca gcagatcctg aaaatgcagc
     2581 gtgccgcaag gctggccctg cgtgttcacc accggcagca cctcgcatcc ccgcgcgaca
     2641 aacagggaga gcgcatcgcg tagcgtcatc tcttctgcca gtgcctcacc ttctgcccgt
     2701 tcttcgcgac gcacgtaatc cgccacacta cgtaacgaaa gcaggcgcac acccagttcg
     2761 ctgcgcccaa aaaactggcg gacaaaatcg tttgccggac gagtcagcat cgtcagcgga
     2821 ttgccctgct gcaccacttc accgtgatcc atcaatacca gatgttctgc cagccgtagc
     2881 gcctcatcaa tatcatgagt gaccagcaca atggtacgcc ccagcaaacg gtgaatgcgc
     2941 gtcatctctt gttgcaacgc gccgcgcgtt accgggtcca gtgcgccaaa aggttcatcc
     3001 atcagtaaga cttgcggatc ggcagccagt gcgcgcgcca ctcccacacg ttgctgctga
     3061 ccaccggaaa gctgatgcgg ataacgctca cgtaaatttg actccagccc cagtagcgcc
     3121 attaattcgt cgatacgatc gtcaatcctc gcccgcgacc atttttgtaa ttgcggcacg
     3181 gtagcgatgt tttgcgccac gctccagtgg gggaacaggc caatagattg aatggcatag
     3241 cccatccggc ggcgcaactc cagtactggc agcgagcgaa tttcttctcc ggcaaagcgg
     3301 atcactccgc tgtcatgctc caccaggcgg ttaatcattt tcagggtggt ggatttgccg
     3361 gagccagatg tgccaatcag caccgaaaaa ctcccttcct gaaaattgag attgagatcg
     3421 ttaacggctt tttgtgcgcc gaacagtttg ctgacatggc caaattcaat cattacgttt
     3481 caccttcagc agtgcgataa gcaaatcgaa cagcgcgtcg gtaagcaccg ccagaacaat
     3541 taccgggatc acccccagca acactaaatc aatggcgctg cttagcagcc cctggaaaac
     3601 cagcgcacca aaaccgcctg cgccgattaa cgccgcaatc accgccatac ctacagtttg
     3661 caccatcacc acccgcaggc tgcgcagaaa taccggtaac gccagtggta actgaacatg
     3721 caggaatcgc tgcgccccgc tcatgcccat cgctctggcg ctctccagca catcgcgcgg
     3781 gatctggttc aaaccgacta ccacgccgcg caccagcggc agcaaggcat agagcaccag
     3841 cgcaatcagt gcgggtgtca ttccggttcc tgctatgccg agcgtcccca gccacggaaa
     3901 ggccgtaacc agcgcggcaa gcggcgcaat caacaggcca aagagcgcca ccgaaggcac
     3961 ggtctgaatg acattgagca gagaaaaaat tgccccctgc cgagcagtgg aaaagtagca
     4021 ccagatgccc aacggcacac caatcactaa cgcaggcagc accgcaccaa acagcaacgt
     4081 cagatgttgt gccagcgcgt cgtcaaacac atcctgacgg ttggcgtatt ctttcattag
     4141 tgagagatcg ttaagcgtgc cggagtacag caaccacagc ggaataatgg caatctgcat
     4201 atgcaacaac cagcgccaca gcggatgcgt ggagattcgg cggatggcat cactacaggc
     4261 cagcaatgcc agcgccgcgg ccagccagaa accactgccg aggctggtac gcgccagcgc
     4321 actgccattt tgcgccagtt gggtcgccgc ctttcctgct ccccacacca gcaatacgaa
     4381 gacgaattgc gccagaatga gtgcaaaaat gctgcctttt ttagcgggaa taaaacaggc
     4441 tgtcagccag gcgcaaccaa cgcccaccag catccagatc gtttgcggcc acagttgcca
     4501 gagatgacgc ccttcacccg agactaaacg attaggcgcg taactgataa acggcagcgc
     4561 cgctgcgatt gccgtcagca acagcagcag cgccagaaca ggattaatac gtagataagt
     4621 cacgggaaat tacttcgtcc accctttttg tttcaggtag tcggcagcca cttttttggc
     4681 atccagtcct tccactgcaa tgctggcatt cagttgctgc aatgtttttg catcgaggct
     4741 ggcgaagact ggctgtagcc actgtgccat ttgcggatac tctttcagca ccgactcacg
     4801 caccactggt gcaggcgcgt agataggttg cacgcctttc ggatcgctta aggtttgcag
     4861 ccccagcgcc gcgaccggac cgtcagtgcc gtaagccatt gcggcattaa cgccagaagt
     4921 ttgctgggca gcggctttaa tcgttaccgc cgtgtcgccg ccagccagtg acagtaactg
     4981 atcctgatcg agtttaaagt cgtaggcttt ttcaaacgcg ggtaacgcat cggcgcgttc
     5041 gataaactct gccgaggctg ccagtttgaa ggtgccgccc tctttcagat aacgactcag
     5101 gtcggcaagc gaagtgagtt tgtttttctc tgccacatcc tgacgcacgg cgatggtcca
     5161 ggtgttattc gctggtgcgg gcgtcagcca gattaacttg ttttgctctg catcgagttt
     5221 tttgactttc tcgtaacctt gctgcgcgtt tttccatgcc gcatcgtttt catctttaaa
     5281 gaagaaagcg ccattaccgg tatattccgg atagatatcc agttcaccgg aagtaatcgc
     5341 cccgcgcacc acaggagtcg ttccgagttg cactttattc accgtcggta cgccgtggct
     5401 ttccagcacc tgcaagatga tgttgccgag tagcgcgcct tcggtatcga tttttgaacc
     5461 gactttaacg ggggaagccg cctgtagcgg caggctcacg gctgccaaca taaccagtga
     5521 acctgcccag acctttgaga gtggcatgat gctttcctca ttcttttact gttgttttca
     5581 gcgaattaag agaaaagcat agttgataat gacagagtta gcctgagcgg aaggattcag
     5641 ttgcagaatc agataaatac ttaaagggca tattttgccc ggcgtttctt gccggatgcg
     5701 gcgtaaacgc cttatccggc ctacaaaggt cgaaaattca gcaagttaag atatgaacat
     5761 caggcaaagg gcgcaaacgt cgcgccctta ctaaagcatt acagcaactc aaactcgcct
     5821 ttcttaacgc gtgcggaatc agtgccgata aagacattga acttgccagg ttcggcgtca
     5881 tatttcatct gttgattcca gaacttcagc gcctcgatat caatcgggaa gctgacagtc
     5941 tgagtttcgc ccggtttcag ggtgattttc tcaaagcctt tcagctgctt cactggacgg
     6001 ctcatggaag ccgtcacatc ctgcaagtac atctgtacta ccgtggcccc ttcgcgttta
     6061 ccggtgttcg tcacctgcac gctggcggtc actttgccgt cacgcttcat ggtcggcgca
     6121 gaaagtttca catcagagac ggtgaaagtg gtatagctca gaccatagcc gaacggataa
     6181 agcgccccgt tagcttcatc aaaataacgc gaagtgtatt tgttcggctt gtcggcatta
     6241 tacggacgac cggtattcag atggctgtag tacaccggga tctgcccgac agaacgcggg
     6301 aaggacattg gcagcttgcc ggacgggttg taatcgccaa acaatacatc ggcaattgca
     6361 ttaccgcctt cagtccccgc aaaccaggtt tccagaatcg catcagcctg ctgatcttct
     6421 ttcaccagcg ccagcggacg cccgttcatc agcaccagca ccagcggttt accggtggct
     6481 ttcagcgccg taatcaagtc acgttggctt tgcggaatag tgatatcggt acggctggag
     6541 gcctcgtgcg ccatcccctg tgcttcaccg accacagcca ccaccacatc agattgtttc
     6601 gccgtctgca ccgcttcatc aatcatctct tgcggcgagc gcggatcgac tttgaccgct
     6661 tcttcatact gattcaggaa atcgataatg cctttgtcac tggtaacgtt cgcccctttg
     6721 gcatacagca ctttaccgtt ttcaccgacg gcatttttaa tcccggtcag tacggtcacg
     6781 gattgatcgg caacaccggc tgcggaccag ctgcccatca cgtcacgttt actgtccgcc
     6841 agtggcccaa ccaccgcaat ggtggccgat tttttcagcg gtaacgtttc gagacggttt
     6901 ttcagcaaca ccaggctttc gcgcgccact tcacgcgctt ctttacggtg caggcggctt
     6961 tcggcattgg tatccaccgg gtcagactct ttcggcccca aatggctgta tgggtcgtta
     7021 aacaatccca tatcatattt aacgttcagt acatggcggg cagcatcgtc cagctcttcc
     7081 atcgtcactt tgccggattt gatcaaccca ggcagatact tcgagtaata ctcgtcgctc
     7141 atactcatgt tgattccgga tttcagcgcc acgcgcaccg catcttccgg gtctgccgcc
     7201 gtgccatgtt taatcagctc tttgattgca ccgtgatcgg aaacggtgat gcctttaaag
     7261 ccccactggt cgcgcagaac atctttcagc agccaggaat cggaggtggc tggcgtgccg
     7321 ttcagcgagt tcagcgccac catcaccgcg ccgctgcctg cgtccagccc cgctttgtac
     7381 ggcggcatat aatcattaaa caggcgctgc ggactcatat cgacggtgtt gtactcttta
     7441 ccgccttcta ccgcgccgta tgcggcaaag tgtttgacgc tggtcatcac cgagtagcga
     7501 tctgccgggc ttttaccctg catcgcttcc accatggttt tacccattgt tgaggtgaga
     7561 tacgtatctt cgccaaaacc ttcggaagca cgtccccagc gcggatcgcg cgagacatcg
     7621 accatcggtg cccaggtcat attcaggcca tcatctgccg cttcataagc agagacacgt
     7681 ccgaccgttt tcactgcatc gaggttaaaa gacgaggcca gaccgaggct aatcgggaac
     7741 accgtgcgct gaccgtgcag cacgtcgtaa gcaaagaaaa gaggaatttt caggcggctt
     7801 aattccatca cctgatcctg catggcgcgg atatcctgac gggttacggt gttgaaaatc
     7861 gccccaacct gaccgtcttt gatcatctcg cggatcgcct ctttcgggtt atccggaccg
     7921 acgctgatta agcgcagctg accaattttc tcatcaactg tcattttctt aagcagttcg
     7981 gtgacgaacg catcccgcgc ttcgggcgtt aatggatggt tgccgaataa atcatccgcc
     8041 agtgctggct gcagggccag actcaccgcg attcctactg aacatagcca tttcatatgg
     8101 atttttcttc ctctgttgcc cgacgaggca gcgaaaatgg tgcaaaaacc gtagtttgcc
     8161 ataagcatga tggagagaga aaaagaatgc tcagtttatt gtctgaattt tcaaaatatt
     8221 cactcgctga attgtcatac aaggcgctat tctagtttgt gatatttttt cgccaccaca
     8281 aggagtggaa aatgtcttcc atgacaacaa ctgataataa agcctttttg aatgaacttg
     8341 cccgtctggt cggtcattca cacctgctca ccgatcccgc aaaaacggcc cgctatcgca
     8401 agggcttccg ttctggtcag ggcgacgcgc tggctgtcgt tttccctggc tcactactgg
     8461 aattgtggcg ggtgctgaaa gcctgtgtca ccgccgacaa aattattctg atgcaggctg
     8521 ccaatacagg cctgaccgaa ggatcgacgc caaacggtaa cgactatgat cgcgatatcg
     8581 tgatcatcag caccctgcgt ctcgacaagc tgcacgttct tggcaagggc gaacaggtgc
     8641 tggcctatcc gggcaccacg ctctattcgc tggaaaaagc cctcaaaccg ctgggacgcg
     8701 aaccgcactc agtgattgga tcatcgtgta taggcgcatc ggtcatcggc ggtatttgta
     8761 acaactccgg cggctcgctg gtgcaacgtg gcccggcgta taccgaaatg tcgttattcg
     8821 cgcgtataaa tgaagacggc aaactgacgc tggtgaacca tctggggatt gatctgggcg
     8881 aaacgccgga gcagatcctt agcaagctgg atgatgatcg catcaaagat gacgatgtgc
     8941 gtcacgatgg tcgtcacgcc cacgattatg actatgtcca ccgcgttcgt gatattgaag
     9001 ccgacacgcc cgcacgttat aacgccgatc ctgatcggtt atttgaatct tctggttgcg
     9061 ccgggaagct ggcggtcttt gctgtacgtc tagatacctt cgaagcggaa aaaaatcagc
     9121 aggtgtttta tatcggcacc aaccagccgg aagtgctgac cgaaatccgc cgtcatattc
     9181 tggctaactt cgaaaatctg ccggttgccg gggaatatat acaccgggat atctacgata
     9241 ttgcggaaaa atacggcaaa gacaccttcc tgatgattga taagttaggc accgacaaga
     9301 tgccgttctt ctttaatctc aagggacgca ccgatgcgat gctggagaaa gtgaaattct
     9361 tccgtccgca ttttactgac cgtgcgatgc aaaaattcgg tcacctgttc cccagccatt
     9421 taccgccgcg catgaaaaac tggcgcgata aatacgagca tcatctgctg ttaaaaatgg
     9481 cgggcgatgg cgtgggcgaa gccaaatcgt ggctggtgga ttatttcaaa caggccgaag
     9541 gcgatttctt tgtctgtacg ccggaggaag gcagcaaagc gtttttacac cgtttcgccg
     9601 ctgcgggcgc agcaattcgt tatcaggcgg tgcattccga tgaagtcgaa gacattctgg
     9661 cgttggatat cgctctgcgg cgtaacgaca ccgagtggta tgagcattta ccgccggaga
     9721 tcgacagcca gctggtgcac aagctctatt acggccattt tatgtgctat gtcttccatc
     9781 aggattacat agtgaaaaaa ggcgtggatg tgcatgcgtt gaaagaacag atgctggaac
     9841 tgctacagca gcgtggcgcg caataccctg ccgagcataa cgtcggtcat ttgtataaag
     9901 caccggagac gttgcagaag ttttatcgcg agaacgatcc gaccaacagt atgaatccgg
     9961 ggatcggtaa aaccagtaag cggaaaaact ggcaggaagt ggagtaaaaa ttacggatgg
    10021 catagtatcg ccatccgtca ttcacttaat cgttctgtgc cgtctgcccc gccgccgcca
    10081 tttgggcggc tttttgtttt ttatagctca acgctgctgc tggaacaggc atcactttac
    10141 cagtttcaat ccaggtacgc aggcggctgg catcggcaaa atgggtatat ttgccaaacg
    10201 catccatcac taccagcgct accggtttat tattgataac cgtacgcatc accagacaat
    10261 ggcccgccgc attggtaaaa ccggttttgg ttaactgaat attccagtta tcgcgataca
    10321 ccagatggtt agtattgcgg aacggcagcg tatacgtcgg gttagagaag gttgccatat
    10381 cttcccgggt agtacttaac tgcccgatca acggatattg tttgctggca atgagcagtt
    10441 tggttaagtc acgggcagtt gaaacgttat gcaccgacaa tccggtaggt tcaacaaagc
    10501 gcgtgttgtt cattccgagc gatttcgctt tcgcattcat tgccttaata aaggctttgt
    10561 aaccaccggg ataatggtgt gcaaggcttg ccgccgcgcg gttttctgaa gacatcagcg
    10621 ccagcaacag catatcttta cggctgattt cgctattcag tcgtacgcgc gaatagaccc
    10681 ctttcatctc cggcgtctgg ctgatatcca cttttagttt ttcatccagc ggcagtcgtg
    10741 catccagcac aaccatcgcg gtcattaatt tgctgataga cgcaatcgga cgcaccagat
    10801 ccgggtggtt cgagtagatc actttgttgg tattcagatc aacaatcatc gcgctaccgg
    10861 aggcaatttc aggttgtgaa gcggtggtag cggctgccgt tttcgcaacg gcctgcggtg
    10921 ccaaaggcac agccagcatc agggccaggc taaataaaga aactcgaaat ttcggcatga
    10981 tgagcattca gatagtggtt cacgcgcaca ggttgcgcac cgccggagta aggatttact
    11041 gaggctagcg acgccatcat aacgagcaaa aagtgcgatc gtcaaaggag aatcgtgagg
    11101 aaatgctgca ttgctgacat ttacgccagc aatgcaacgt caaagaactt tctagaacaa
    11161 acgataaccg tagccccata gtataacggt tagggcgagc agcacttcca gtaccagcac
    11221 gccaatcgcc agcgtcgaac tagagaagct aaggccttcc tctttgttga tattcaggaa
    11281 gctcggaata ccaaggtaaa gcaggtagcc ggtgtaaaac agcgccaccg tgccgaccag
    11341 cgcacacaac cagaccagtg gataaagcgc caccagaccg cttaaaaaca gcggagttgc
    11401 aacgtagccc gcgaagacca tgcagtgcgc aagtgacgga cgctgcggat aattacgcgc
    11461 catccaccag atgacccgcc ccattaccgc aaccccagcc agcataacgc cataaaacag
    11521 gacagccagc gccagtccgg taaaccagga taacttcagg atagtgccat cgccaaaatt
    11581 ccagccaatc tgtgtagtgc caatgaaggc gcaaatcacc ggaatcgccg ccatcagcaa
    11641 aacgtggtgg gtgtaatggt gagaaatcgt ttcgttttcg cgattaatca cctgcatttc
    11701 acgatcggga tgggaaaaca gtccccagac atggctcata ccgccccctt gttgtgagtt
    11761 catgaacctg acagttcaag tataagtcag cttgtgatta ttttttgttc gctcatgtaa
    11821 tttgcaccgt ctgatgtacg tcctttttca gcgtgaatga tttccagggt gtatgcttac
    11881 aggcaaccaa agggagacag actggcctat ggatctcaat acactcatct cacaatatgg
    11941 ttatgccgcg ctggtgatcg gtagcctggc ggaaggtgaa accgtgactt tgctgggagg
    12001 cgttgcggca catcaggggc tattaaagtt cccgctggtg gtactttctg tggcgcttgg
    12061 cggcatgatt ggcgaccagg tgctttatct gtgcgggcgg cggtttggcg gcaagctgtt
    12121 acgccgtttc tcgaaacatc aggataaaat tgagcgggcg cagaaactta tccaacgcca
    12181 tccgtatctg tttgtcattg gtacgcgctt tatgtatggc tttcgggtga ttggcccgac
    12241 gctgattggt gccagccagc tgccgccgaa aatctttctg ccgctgaata ttctcggcgc
    12301 atttgcctgg gcgttgattt ttaccactat tggttacgct ggtggtcagg tgattgcgcc
    12361 gtggttgcac aatctcgacc agcatttgaa gcactgggtc tggttgattc tggtcgtggt
    12421 tctggtggtg ggcgtgcgct ggtggctgaa acggcgcggg aagaaaaagc cggatcatca
    12481 ggcgtaaaac cattgccctg ggtaaggcgt tcgcgccgca tccgacatcc ggtgcaccat
    12541 gcctgatgcg acgcttgtgc gtcttatcat gcctaccaaa attttaccca ttgaaataac
    12601 cacgcacctc actgcgctat tctgggttga actgcggatt cgccaacata aagccgccat
    12661 ccactatcaa cgactgcccg gtggtgtaat ttgcgccctc tgagcacagc cacgctacca
    12721 ggctggcaat ctcatgcgtt gcgccaaaac gccgcagagg aatcgaaggc tccgcgtcgg
    12781 gcttcacgtc gctgtcatcc atgccattca ttggcgtggc gatcgcccca ggcgcgactg
    12841 cgttcaccaa aatcttatgc ctgaccagct ccagcgccat cgttttggtt aacccgccga
    12901 gcgcatgttt agcggctgtg taagcgctgg catccggcag cggcgtatgt tcatgtaccg
    12961 acgtaatgtt gatgatacga ccgccctgcc cttgtttcac catctggcgg gccgcaattt
    13021 gtgagcataa aaatgcacca tcgacatcaa cggtaaaaat cttgcgccac tcatcgaaag
    13081 ccatatcaag aaacggcgct ttggtcattg cgcccgcatt attcaccagc acatcaatac
    13141 gccccagccg ttgaatgagt ttctccagcg cctgcgcacc ttctggcagt ttaccgagct
    13201 ccagctgcac gatctccgca cgtacgccgt ggctaactac ctcacgcgcg gtatcttttg
    13261 ccccttcttc atctgagtgc caggtaatgc caatatcaaa cccctgctgc gccagtaata
    13321 acgcgcactc tttgccgatc cccgaatcgg aggcggtaat aatcgcaacc tgtgccatca
    13381 agttctccac ttaacgctga ataaacgtta agtatagaag gcgcatatca tcagcgtttg
    13441 taacccccgc ccaacgcacc agtgagttga atagaagcat ccagccactg cccttgcaat
    13501 aacaggccat tggcccgctc acgcagcgcg gggattctgg cttcgctgac gcgggaacca
    13561 acaatgatgc ccgcgttaaa gcgcgcctgc gcaagaccta ccacacgcaa ggcatcgcgc
    13621 tcaatttgcg cctgatgctg gtttttctcc gcaagtgtct gaacctgact ggctgcccgc
    13681 gccacgtcat tcaccgcttc aaccaccgct ttgttgtagc tggcgataga caagttgctt
    13741 tcggcttttg cgatattgag attggcgtta agacgaccac tatcgaaaat gggtagcgtc
    13801 aggcctgccg taacgcccat ttgctgcgcg gaatgacgga acagatcgct taagtgcaac
    13861 gcatcctgtt gcaggaaggc catcaggttg atgtcaggat aaaatgccgc ttttgccgca
    13921 tcaatggtgc ttagcgatga ctcaacgtac cagtgcgccg cctgcaaatc tgcccgccgg
    13981 gccagtaagg agtaccccag ttcatcagga agctggcttg ccactttcgg caacgcgacc
    14041 ggtttaagct tcaatgactt tgtctggtta tttgtaagtg cgcttaaccg tgcctcaata
    14101 attttcattt tccccgcgac atcgttgagc tgctgccggg ttttgctggc attaatatcg
    14161 gtttccacac cttcaactga agaagtaatc ccgttctgat atagctggcg atcggtcgcg
    14221 ataatggtgt tctgctcttt ttctatttgc tgcaagaccg tgtttaacgc cgcctgggtt
    14281 tgccactccc agtacaggcg ggctacgctg ccagccagca attggcgggt ttgctcgcgt
    14341 tccgccgccc gtgctttaac cgtacccagg cgggcagtaa cctccgcccg attctttccc
    14401 cagatatcga gatgccagcc cgccgttaag ccaaaagtac cgttggtgta ccacgggccg
    14461 gtcgtacctg cggccggatc gttcagagca aacggcccca ttaagccttc tgccgacatt
    14521 ttttgccgct ccatatccgc cgaaaagtcg atctgcggac catcctgagt ggcaactgcc
    14581 ttcgcctggg cttcagctag ctgaatgcgc tgttcagcca cctgcatatc cggtgcgttc
    14641 tgtagtgcat tgttaattaa tgaagtgagc tgattatcgt gatactccag ccaccattgg
    14701 ctgtctggcc aaccattttt cagcgccgtg ggtaatgcgg tgtcaacttg tgcagcgggc
    14761 gtttgctggc ttaacgcctg gcgggtttca tgcataggcg cacacccggc cagcatcagt
    14821 aacagcggaa aacaggcgat ggctggataa aaggaatcac gattcatggg ggaataatca
    14881 ggtaagaaac ggtgcgcgga gattaccgtg tgttgcgata tattttttag tttcgcgtgg
    14941 caatacatca gtagcaataa aacgacatac ccagaaaaat atagactaag taaatgatat
    15001 cttccgattt atcttaatcg tttatggata acggcaaagg gcttcgtttt ttcctatact
    15061 tattcagcac tcacaaataa aggaacgcca atgaaaatta tactctgggc tgtattgatt
    15121 attttcctga ttgggctact ggtggtgact ggcgtattta agatgatatt ttaaaattaa
    15181 ttaatgtcat caggtccgaa aataacgaga atatttcagt ctctcatcct gttgcgctcc
    15241 tgtcatg
//
LOCUS       DAJTZL010000097        15235 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.097, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000097 DAJTZL010000000
VERSION     DAJTZL010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15235
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.097"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..749
                     /locus_tag="OO544_002603"
     CDS             <1..749
                     /locus_tag="OO544_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF945 family protein"
                     /protein_id="HCR6768169.1"
                     /translation="SKFNQINIINHGEDVAQIDAFVAKTMLDRVKDKDYINVNLTYEL
                     DKLTKGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAVHPELANDEVALQEV
                     NAALFKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKLLN
                     FDVKLPLNVVTETAKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTASL
                     QLRYTPGKVVFNGQEMSEEEFMSRAGRFVH"
     gene            complement(790..1722)
                     /locus_tag="OO544_002604"
     CDS             complement(790..1722)
                     /locus_tag="OO544_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCR6768170.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQNK"
     gene            2086..4872
                     /gene="polA"
                     /locus_tag="OO544_002605"
     CDS             2086..4872
                     /gene="polA"
                     /locus_tag="OO544_002605"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HCR6768171.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            complement(5254..5886)
                     /locus_tag="OO544_002606"
     CDS             complement(5254..5886)
                     /locus_tag="OO544_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HCR6768172.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            6468..6977
                     /gene="yihI"
                     /locus_tag="OO544_002607"
     CDS             6468..6977
                     /gene="yihI"
                     /locus_tag="OO544_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HCR6768173.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVAEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            7166..8539
                     /gene="hemN"
                     /locus_tag="OO544_002608"
     CDS             7166..8539
                     /gene="hemN"
                     /locus_tag="OO544_002608"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HCR6768174.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(8729..8839)
                     /locus_tag="OO544_002609"
     CDS             complement(8729..8839)
                     /locus_tag="OO544_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HCR6768175.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(8951..10360)
                     /gene="glnG"
                     /locus_tag="OO544_002610"
     CDS             complement(8951..10360)
                     /gene="glnG"
                     /locus_tag="OO544_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HCR6768176.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            complement(10372..11421)
                     /gene="glnL"
                     /locus_tag="OO544_002611"
     CDS             complement(10372..11421)
                     /gene="glnL"
                     /locus_tag="OO544_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HCR6768177.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            complement(11595..13004)
                     /gene="glnA"
                     /locus_tag="OO544_002612"
     CDS             complement(11595..13004)
                     /gene="glnA"
                     /locus_tag="OO544_002612"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HCR6768178.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            13377..15200
                     /gene="typA"
                     /locus_tag="OO544_002613"
     CDS             13377..15200
                     /gene="typA"
                     /locus_tag="OO544_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HCR6768179.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
BASE COUNT         3965 a         3803 c         3914 g         3553 t
ORIGIN      
        1 agtcgaaatt taatcagatt aacattatca atcacgggga agacgttgcc caaatcgatg
       61 ctttcgttgc aaaaaccatg ctggatcgcg ttaaagacaa agattatatc aatgtcaatc
      121 tgacctacga acttgataag ttaacaaaag ggaatcagca actcggtagt ggtgagtggt
      181 cattgattgc tgaatctatt gatccctcag cagtgcgcca atttatcatc cagtataaca
      241 ttgctatgca gaagcagctt gctgtacacc ctgagttagc aaacgatgaa gttgctctgc
      301 aagaagtgaa tgctgcattg ttcaaagagt atttaccgtt attacaaaaa agtgagccga
      361 ccattaaaca accggtaaga tggaagaacg cactcggcga actaaatgcc aatctggata
      421 tcagtattgc cgacccagcc aaatcttcat catccacaaa caaagatatc aaattgctca
      481 attttgatgt gaagttaccg cttaatgtcg tcacagaaac cgcaaaacag cttaatttat
      541 ctgaaggaat ggatgcggaa aaagcgcaaa agcaggctga taaacaaatc agcgggatga
      601 tgacattggg tcagatgttt cagttaatca cgattgacaa caataccgcc tcgctgcagc
      661 tgcgttatac accgggtaaa gttgtgttta acggacagga gatgagcgaa gaagaattta
      721 tgtctcgtgc cggacgtttt gttcattaaa atactcactc gcctctgctc attcagaggc
      781 gagtaaaact tacttattct gacgctgtga tgacatgaga gacgtcagaa gccggtcttt
      841 ttcctgccac aatgagttca gccattgctg aaaatggcgc ttaaagcttt tgtcattgat
      901 gtagtcgcca tgtaactcat cggcaatagg ctgtaaatcc acatgtacga caatccgcgt
      961 cagtttaccg cttaacatat cgaagaacgg ctgacggtta ttgtccggat aacacagcgt
     1021 aacattaagc agtttatcga attgtttacc cagtacattt agcgccatcg caatgcctgc
     1081 cgcctttggt ggcaacaagt tttgaaaagt ggagtgggtt tgctgatgtt tttcttgcgt
     1141 gaaacgggag ccttcaacga aattgacaat ggtggtggga tgcaggcgaa acttttcaca
     1201 agaacgccga gtggtttcaa catctttacc acgtcgctcc ggatgacgta ataaataagc
     1261 gcgggaataa cgcttcataa acggcatatc cagcgcccag cacgccaggc caagaaatgg
     1321 cacccaggcc agctgttgct tgaggaaata tttattcatc ggaatgtgct tacgaaacag
     1381 cacgcacagt acgacaatat ctgcccaact acggtggtta caaataagca gataccagtt
     1441 cttcttactt aacccttcca gcccgtgaac ttcccattgc aggtgtgggt tcagatgcag
     1501 taataccgcc agaccttcac accagcaata catcataaaa tcacaaaaac gcgaaacctt
     1561 tcgccaaatg actggcacag gtagcaatag ttttacaatc ccggcaatga tgatcgggac
     1621 ggaacaaaaa atagtgacca aaatggtcaa tacgatactc agcaaaaggg ttatcgcagc
     1681 gagtattctc gtcataatga atttattcaa aaggttagcc atagacagtg cgctaaagaa
     1741 gcgcaaggcg cagattttag cagaaaacga cccaaataac ggatgatcct taaggagaaa
     1801 aataatccat atctatccac attagaaaaa atcccattat ctcaattatt agggatggct
     1861 ttatttttaa ctgcatgaaa aacaaagaca aacatcatgc tgtaaaaagc atgataataa
     1921 attaaaagcg atgtaaataa tttatgcaca aagttatcca catgacgatt tgcgagcgat
     1981 ccagaagatc tacaaaagat tttcacgaaa agcggtgaaa aactcatgtt ttcatcctgt
     2041 ctgtggcatc ctttacccat aatctgataa acaggcacgg acattatggt tcagatcccc
     2101 caaaatccac ttatccttgt agatggttca tcttatcttt atcgcgcata tcacgcgttt
     2161 cccccgctga ctaacagcgc aggcgagccg accggtgcga tgtatggtgt cctcaacatg
     2221 ctgcgcagtc tgatcatgca atataaaccg acgcatgcag cggtggtctt tgacgccaag
     2281 ggaaaaacct ttcgtgatga actgtttgaa cattacaaat cacatcgccc gccaatgccg
     2341 gacgatctgc gagcacaaat cgaacccctg cacgcgatgg ttaaagcgat gggactgccg
     2401 ctgctggcgg tttctggcgt agaagcggac gacgttatcg gtactctggc gcgcgaagcc
     2461 gaaaaagccg ggcgtccggt gctgatcagc actggcgata aagatatggc gcagctggtg
     2521 acgccaaata ttacgcttat caacaccatg acgaatacca tcctcggacc ggaagaggtg
     2581 gtgaataagt acggcgtgcc gccagaactg atcatcgatt tcctggcgct gatgggtgac
     2641 tcctccgata acattcctgg cgtaccgggc gtcggtgaaa aaaccgcgca ggcattgctg
     2701 caaggtcttg gcgggctgga tacgctgtat gccgagccag aaaaaattgc tgggttgagc
     2761 ttccgtggcg cgaaaacaat ggcagcgaag ctcgagcaaa acaaagaagt tgcttatctc
     2821 tcataccagc tggcgacgat taaaaccgac gttgaactgg agctgacctg tgaacaactg
     2881 gaagtgcagc aaccggcagc ggaagagttg ttggggctgt tcaaaaagta tgagttcaaa
     2941 cgctggactg ctgatgtcga agcgggcaaa tggttacagg ccaaaggggc aaaaccagcc
     3001 gcgaagccgc aggaaaccag tgttgcagac gaagcgccag aagtgacggc aacggtgatt
     3061 tcttatgaca actacgtcac catccttgat gaagaaacac tgaaagcgtg gattgcgaag
     3121 ctggaaaaag cgccggtatt tgcatttgat accgaaaccg acagccttga taacatctct
     3181 gctaacctgg tcgggctttc ttttgctatc gagccaggcg tagcggcata tattccggtt
     3241 gctcatgatt atcttgatgc gcccgatcaa atctctcgcg agcgtgcact cgagttgcta
     3301 aaaccgctgc tggaagatga aaaggcgctg aaggtcgggc aaaacctgaa atacgatcgc
     3361 ggtattctgg cgaattacgg cattgagctg cgtgggattg cgtttgatac catgctggag
     3421 tcctacattc tcaatagcgt tgccgggcgt cacgatatgg acagcctcgc ggaacgttgg
     3481 ttgaagcaca aaactatcac ttttgaagag attgcgggta aaggcaaaaa tcaactgacc
     3541 tttaaccaga ttgccctcga agaggccgga cgttacgccg ccgaagatgc ggatgtcacc
     3601 ttgcagttgc atctgaaaat gtggccagat ctgcaaaaac acaaagggcc gttgaacgtc
     3661 ttcgagaata tcgaaatgcc gctggtgccg gtgctttcac gcattgaacg taacggtgtg
     3721 aagatcgatc cgaaagtgct gcacaatcat tctgaagagc tcacccttcg tctggctgag
     3781 ctggaaaaga aagcgcatga aattgcaggt gaggaattta acctttcttc caccaagcag
     3841 ttacaaacca ttctgtttga aaaacagggc attaaaccgc tgaagaaaac gccgggtggc
     3901 gcgccgtcaa cgtcggaaga ggtactggaa gaactggcgc tggactatcc gttgccaaaa
     3961 gtgattctgg agtatcgtgg tctggcgaag ctgaaatcga cctacaccga caagctgccg
     4021 ctgatgatca acccgaaaac cgggcgtgtg catacctctt atcaccaggc agtaactgca
     4081 acgggacgtt tatcgtcaac cgatcctaac ctgcaaaaca ttccggtgcg taacgaagaa
     4141 ggtcgtcgta tccgccaggc gtttattgcg ccagaggatt atgtgattgt ctcagcggac
     4201 tactcgcaga ttgaactgcg cattatggcg catctttcgc gtgacaaagg cttgctgacc
     4261 gcattcgcgg aaggaaaaga tatccaccgg gcaacggcgg cagaagtgtt tggtttgcca
     4321 ctggaaaccg tcaccagcga gcaacgccgt agcgcgaaag cgatcaactt tggtctgatt
     4381 tatggcatga gtgctttcgg tctggcgcgg caattgaaca ttccacgtaa agaagcgcag
     4441 aagtacatgg acctttactt cgaacgctac cctggcgtgc tggagtatat ggaacgcacc
     4501 cgtgctcagg cgaaagagca gggctacgtt gaaacgctgg acggacgccg tctgtatctg
     4561 ccggatatca aatccagcaa tggtgctcgt cgtgcagcgg ctgaacgtgc agccattaac
     4621 gcgccaatgc agggaaccgc cgccgacatt atcaaacggg cgatgattgc cgttgatgcg
     4681 tggttacagg ctgagcaacc gcgtgtacgt atgatcatgc aggtacacga tgaactggta
     4741 tttgaagttc ataaagatga tgttgatgcc gtcgcgaagc agattcatca actgatggaa
     4801 aactgtaccc gtctggatgt gccgttgctg gtggaagtgg ggagtggcga aaactgggat
     4861 caggcgcact aagattcgcc tgaacatgcc cttttttcgt aagtaagcaa cataagctgt
     4921 cacgttttgt gatggctatt agaaattcct atgcaacaac tgaaaaaaaa ttacaaaaag
     4981 tgctttctga actgaacaaa aaagagtaaa gttagtcgcg tagggtacag aggtaagatg
     5041 ttctatcttt cagacctttt acttcacgta atcggatttg gctgaatatt ttagccgccc
     5101 cagtcagtaa tgactggggc gttttttatt gggcgaaaga aaagatccgt aatgcctgat
     5161 gcgctatgtt tatcaggcca acggtagaat tgtaatctat tgaatttacg ggccggatac
     5221 gccacatccg gcacaagcat taaggcaaga aaattattcg ccgtcctgcg tttcttctac
     5281 aggctgcatc tcgctaaacc aggtatccag tttctgccgc agcttgtcca cgccttgttt
     5341 cttcaacgaa gaaaacgttt caacctgcac atcaccgtta aacgccagta cagcttcacg
     5401 caccatattc aattgcgctt tacgtgcgcc gcttgccagt ttgtccgctt tggtcagcag
     5461 caccagaacg gcgatattgc tgtctaccgc ccactcaatc atctgctgat ccaaatcttt
     5521 cagcggatgg cgaatatcca ttagcaccac cagaccttgc aggctctgac gtttttcgag
     5581 gtattcgccg agcgcacgct gccatttgcg cttcatctct tccgggactt ccgcataacc
     5641 gtacccaggc aagtcaacca gacgcttgcc gtcagccact tcaaacaggt tgataagctg
     5701 ggtgcgccct ggggtttttg aggtacgagc caggcttttc tggttagtca gcgtgttcag
     5761 cgcgctggat ttacctgcgt tggaacggcc tgcaaaagcc acttcaattc cggtatcgga
     5821 aggtaggtgg cgaatatcag gcgcactcat cacaaaatgc gtctgttgat aattcaaatt
     5881 agtcaaagcg gtcgtctccg tcagtcaaag ctttgtggcg attatacctg aaccataata
     5941 aaaggcggat ttttcggcgt gagcgttgta agtaaaagcc atacgctttg tgagacattg
     6001 ccgatagtct ttatgcgaaa tagcagagaa aattctgcga tgcatgtcaa ataagctata
     6061 taaatcagtg aattgctttt atataaaact ctgaaaatgt gcaaatactg atggcggttg
     6121 attgtttgtt taaagcaaag gcgtaaagta gcacccatag agcgaggacg ctaacaggaa
     6181 caatgactca ggatgagggt caggagcgcc aggaggcgaa gacagaggat tgtcaggaag
     6241 acaaacgtcc ggagacgtaa ttaaacggaa atggaatcaa cacggattgt tccggctaaa
     6301 ggaaaaacag ggtgtgttgg cggcctgcaa ggattgtaag acccgttaag ggttatgagt
     6361 caggaaaaaa ggcgacagag taatctgtcg ccttttttct ttgcttgctt tctgttagat
     6421 tccgccgcaa atctatactg aataaaacgg ctaaaagacg aaccattatg aaaccatcat
     6481 cttcaaactc acgcagcaaa ggtcatgcaa aagcgcgtcg aaaaacacgc gaggagctgg
     6541 atcaggaagc tcgtgatcgc aagcgtcaga aaaaacgtcg tggtcatgcg ccgggcagcc
     6601 gcgcagcggg cggaaacacc acgtcaggca gcaaaggcca gaacgcacca aaagatccac
     6661 gtattggcag taaaacccct attccattgg gcgtggctga aaaagtcacc aaacagcaca
     6721 aaccgaagag tgagaaacct atgctttcac cgcaggcgga gttggaatta ctggaaacgg
     6781 atgagcgtct ggatgcgctg ctggaacgtc tggaagcagg cgaaaccctg agcgccgaag
     6841 agcaatcctg ggtggatgct aaactggatc gtattgatga gttgatgcag aaactcggcc
     6901 tctcttatga cgatgacgaa gaagaggaag aagacgagaa gcaagaagac atgatgcgtc
     6961 tgctgcgggg taactaacgg attgccaccg tgggccttcc cgttctgctg ataacccttc
     7021 cggttatatg ttatctggtg tggttattcg ttaaactaca gcggttgtcg cggcgacaaa
     7081 agtggctgcg caaccggctg atgacccgaa acgggcatcc gccggtacgc cgtagccgcc
     7141 agagacgcca tcggaaggag tgagcatgtc tgtacagcaa atcgactggg atctggccct
     7201 gatccagaaa tataactatt ccgggccacg atacacctcg tacccgaccg cgctggagtt
     7261 ttcagaagac ttcggcgaac aggcgttttt acaagccgtg gcgcgctatc ctgagcgtcc
     7321 attatctctc tacgtacata tcccgttctg ccataagctt tgttacttct gcggttgcaa
     7381 taagattgtt actcgccagc agcacaaggc cgatcagtat ctggacgcgc tggagcaaga
     7441 aatcgtccat cgtgcaccgc tgttcgccgg gcgtcacgtc agccaattgc actggggcgg
     7501 cggaacgccg acgtatctga ataaagcgca aatcagccgc ctgatgaagc tgctgcgcga
     7561 aaacttccag ttcaatgccg atgcggagat ttcgatcgaa gtcgatccgc gggaaatcga
     7621 actggatgta ctcgatcatt tacgcgccga aggctttaat cgcctgagca tgggcgtgca
     7681 ggacttcaac aaagaagtgc aacgtctggt taaccgcgag caggatgaag agttcatctt
     7741 tgcactgctt aaccatgcgc gtgagattgg ttttacctcc accaacatcg acctgattta
     7801 cggcctgccg aaacagacgc cggagagttt cgcctttacc ctgaaacgtg tggcggagct
     7861 gaaccccgat cgtctgagtg tctttaacta cgcgcatctg ccgaccattt ttgctgctca
     7921 gcgcaaaatc aaagatgctg acctgccgag tccgcagcaa aaactcgata tcctgcagga
     7981 aaccatcgcc ttcctgacgc aatcgggcta tcagtttatc ggtatggatc actttgcccg
     8041 tccggatgac gagctggcgg tggcccagcg tgaaggcgtg ctgcatcgta acttccaggg
     8101 ctacaccact cagggcgata ccgatctgct ggggatgggc gtttccgcca tcagcatgat
     8161 tggcgactgc tacgcgcaga accagaaaga gttgaagcag tactatcagc aagtggatga
     8221 acaaggcaat gctctgtggc gtggtattgc gctaacgcgt gatgactgta ttcgccgcga
     8281 tgtgattaag tcgctcatct gcaacttccg tctggattac gcccctattg agaaacagtg
     8341 ggatttgcac ttcgctgatt actttgcgga agatctcaag ctgctcgccc cgttagcaaa
     8401 agatgggctg gtggatgtgg atgagaaggg aatacaggtg acggcgaaag gtcgcttgct
     8461 gatccgcaac atttgcatgt gctttgatac ctatctgcgc cagaaagcgc ggatgcagca
     8521 gttctcacgg gtgatttaaa tagtgaatgg cgcttcgttt acaaagtaga aaacgaaagc
     8581 gccatcaatg tgctgagaac aagattgcct gatgcgctac gcttatcagg cctacaaaac
     8641 ctgttgaaaa tattgctttt gtaggccgga taaggcgttc acgccgcatc cggcatgaac
     8701 aaagagcact tcatcagcaa tctgaaactc aactaaacag cccaatcatt gcggcacaca
     8761 gcacggcagc aacggcagtt tgcggtgtgt ggctgtcaac tgcgccactc gataccagat
     8821 taattattga ttccagcata atgactctcc ccgttttccg ggcaagatca tactgaactt
     8881 atcggaacag taaagcgtaa aataccagca atttgcgctc aataatcaat ctttacacac
     8941 aagctgtgaa tcactccatc cccagctctt ttaacttacg cgtcagggtg ttgcggcccc
     9001 agccgagtag ccgcgccgct tcctgtttat gcccctgcgt atgtcgtaac gcggtcgtca
     9061 gtaacgtccg ctccagctct ggctgcgctt cggaaagcag attttgatga ccggaacgca
     9121 gcgctctgtc tgcccactgc gcaagaagcg ttgcccagct gtccggttgc atttgcgaag
     9181 tactctccgc aaccgttgat tcaaacagtt cgccgggcaa atcctgaatc aacacttcct
     9241 gcccggcggc catcaccgtt agccagcggc aggtgttttc cagctggcgc acgttgcctg
     9301 gccacgccag acgcgtcaga gcagcttcgg tttccggatg cagtaacttc gcttctacgc
     9361 ccagttcgcg cgcggcaacc tgtaaaaaat ggcgcgccag acggggaata tcttcccgac
     9421 gttcgcgcag cggcggcaga tgaacgcgga taacgttcag gcggtggaac agatcctcac
     9481 ggaacttacc ttcctgcact cgctgttcga gattctggtg agtggcagcg ataatacgca
     9541 catccacttt caccggcgca tagccgccaa cgcggtaaaa ctgaccgtct gccagcacgc
     9601 gcagcaaacg cgtctgcaca tccagcggca tatcaccaat ttcgtcgagg aataatgtac
     9661 cgccatcggc ctgttcaaaa cgcccctgac gaatggtatt cgcgccagta aacgcgcctt
     9721 tctcgtggcc aaacagttct gattcgatca aatcttttgg gatagctgcc atattcagcg
     9781 cgataaacgg cgctttggcg cgcggactgt ggcgatgcag ggcatgagcg accagttctt
     9841 taccggtgcc ggattcgccg ttaatcagca cgctaataga agaacgcgaa agccgaccga
     9901 taatacggaa cacgtcctgc atggctggcg cttcgccgat gatatcggtc gttgggccgt
     9961 taagctgaat attacgcggc tgctgctgtt cctggtaatg actgatagcg cgctcaacca
    10021 gcgccactgc ttcgtcgata tcaaacggtt tgggcagata atcaaacgcc ccttgttgat
    10081 aggcgctgac ggcagcatcc agatcggaat gtgcggtcat aatgatgacc ggaagcattg
    10141 gatggcgctg tttaatctgc ttgagcagcg ccagcccgtc cattcccggc atacggatat
    10201 ctgaaagcag cacatccggc gttttgctcg ccagcgcctc cagtacttcc gcgccgttct
    10261 caaatgtcgt acaggttaaa cccgctccag cgagcgcacg ttcaagcacc caacggatgg
    10321 aactatcgtc atcgactacc cagactatcc ctcgttgcat aaacgtcacc tttatttcct
    10381 gataggcagg taaaccgaga actcggtatg acctggccaa ctggtaaatt caattttgcc
    10441 tgaatgctga tcaatcaaat tacgggcgat ggataagcca agcccggtgc caccttcgcg
    10501 gccgctgacc atcgggtaaa acagcgtatc ctgcaaatga ggcggaatgc ccggcccgtt
    10561 atcttccaca tcaatccgcg ccgccagccg gtagcgctcg ccgtgtaagg tcagttgaaa
    10621 cgcggtgcgg gtacgcagaa tgatttcacc gccttccggc cccagcgcct gtagcgcatt
    10681 gcgcacaata ttcagcaaga cctgttcaat ttgatccggg tcgtgcgcca gttccggcag
    10741 gctggggtcg taatcacgaa tcaaccgcac gttgtccggc agttccatcg acaccagcgt
    10801 caccacgcgt tcagctactt tgtgaatact ttcggtaacg cgcgtaccgg gcagctgcgg
    10861 ccccaacaga cggtcgacca gatttcgcag ccggtccgcc tgttcgataa tcactttggt
    10921 atattcgagt agtgatgggt caggtaacgc tttgctgagc agctgcgccg cgccacgtaa
    10981 acctccaagc ggatttttaa tctcatgtgc caggccgcgc actaaatcac gggcagcaac
    11041 ctgctgggcg tgctgtagct gttcctgact taagcggcgc tggttatcca tcggagccat
    11101 ctccagcagg atcatgccgt ccggcatacg ctgggccgtc acagaaagga tatgcgagcg
    11161 cccgtcgatg accagcgtca cttcgttatc ggtaaaacct tgccccgcct ccagactttc
    11221 ttgcatcagc tcgatattta atgagaagta gctcaacagt tccggtaacg gtgtaccaaa
    11281 caatttgcgg gagctttggg cgagcagttg ttgcgcggcg gggttggcgt aatggatcgc
    11341 cagattgtca tcaattaaca aaatactgtt aatcagcgag ttgaggatct gcccagcatc
    11401 gggctgcgtg cctgttgcca taaagcagtc tcctgaacag gttgcaccat tttagtgcat
    11461 tatagctttt tacggataaa aagcgcgaag catcagagaa ttgacggaga aaaaagccca
    11521 tgcagagatg ggctgaaagt ttccacggca actaaatccc ggcgttgttg cgccgggtag
    11581 tacaacttca actattagac gctgtagtac agctcaaact ctaccggatg cggagtcata
    11641 cgcacgcggt catcttcttc gcgacgtaga gcgatgtacg catcaattgc ttcgtcagtg
    11701 aacacgccac cggctttcag gaactcgcgg tccagatcca gttcgttcag tgcttcttcc
    11761 agagagcctg caacctgtgg gatctctttc gcttcttctg gcggcaggtc atacaggttt
    11821 ttgtccatgg cttcgcccgg atggatcttg ttcttgatac catcaagacc ggccatcagc
    11881 agggcagcaa agcacaggta cgggttagct gccggatccg ggaaacgtac ttcgatacga
    11941 cgtgctttcg gagaagaaac caccggaata cggatagacg cagaacggtt acgcgcagag
    12001 taagccagca ttaccggtgc ttcatagccc gggaccagac gcttataaga gttggtggtc
    12061 gggtttgcca gggcgttaat cgctttagcg tgtttgatta cgccgccaat gtagtacagc
    12121 gcctgctcag acagacctgc gtatttgtcg cctgcgaaca ggttaacgcc gtttttagac
    12181 agagacatgt ggcagtgcat accggagccg ttatcaccga acatcggttt tggcataaag
    12241 gtcgcggttt taccgaagcg gtgcgctacg ttgtgcacaa catatttgta gatctgaatt
    12301 tcgtcagctt ttttggtcat agtattgaag cgggtagcca cttcgttctg accagcagtc
    12361 gctacttcgt ggtgatgggc ttcaaccacc agacccatct gttccatcac cagacacatt
    12421 tcagaacgaa tatcctgagc cgagtctacc ggtggaaccg ggaagtaacc gcctttcact
    12481 gccggacggt gacctttgtt accaccttcg tattgggtgg aggagttcca tgcgccttcg
    12541 atatcgtcga tagcaacgtg ggaaccggag atagatgatc cgaaacggat gtcatcgaac
    12601 aggaagaatt caggttctgg cccgaacagt acggtgtcgg caatgccagt ggaacgcagg
    12661 taatcttcgg cgcgcttcgc aatggagcgc gggtcacggt catagccttg cagggtgcca
    12721 ggttcaagga tgtcgcaacg gataatcagg gtggagtcgg cgaagaacgg gtcaatcact
    12781 gcggtggatg cgtctggcat cagcaccatg tcggactcgt taatgccttt ccagccgcca
    12841 atcgaggagc cgtcaaacat tttgccttct tcgaagaatt cagcattcac ctgatgagca
    12901 gggatagtga cgtgctgttc tttaccttta gtatcggtga agcgcaaatc aacaaacttc
    12961 acttcgtgct cgttcagcat cgtcagtacg tgttcagcgg acatacttta actctcctgg
    13021 attggtcatg gtcgtcgtgg taacgaaatc tgcaattatt ttggccgtgt cgccgtaaaa
    13081 aagataaagc gaaatctgtg ccaactttta aattgcccct aaaaggcgtt atcatgcgca
    13141 ccatcgtgca aaagggctgc accacgatgt gaatgttgca ccaatatagt gcttcaatgg
    13201 aaacattaag caccatgttg gtgcaatgac ctttggataa ccctttttat gctccgtgaa
    13261 agcgatcaca aagggactct gcaatacttg tttgcggagg atgtttgtga tcctgttttg
    13321 tagtgcgatt aatccgtgta caataacgcg ctatttctaa tgcctgaggc aaagttgtga
    13381 tcgaaaaatt gcgtaatatc gccatcatcg cgcacgtaga ccatggtaaa accaccctgg
    13441 tagacaagct gctccaacaa tccggtacgt tcgactctcg tgccgaaacc caagagcgcg
    13501 tgatggactc caacgatttg gagaaagagc gtgggattac catcctcgcg aaaaacaccg
    13561 ctatcaaatg gaatgattac cgtatcaaca tcgttgatac cccggggcac gccgacttcg
    13621 gtggtgaagt tgaacgtgta atgtccatgg tagactcagt gctgctggtg gttgacgcat
    13681 ttgacggccc gatgccgcaa acgcgcttcg taaccaaaaa agcgtttgct tacggcctga
    13741 agccgattgt tgttatcaac aaagttgacc gccctggcgc gcgtcctgat tgggttgtgg
    13801 atcaggtatt cgatctgttc gttaacctcg acgcgaccga cgagcagctg gacttcccga
    13861 tcgtttacgc ttctgcgctg aacggtatcg cgggtctgga ccacgaagat atggcggaag
    13921 acatgacccc gctgtaccag gcgattgttg accacgttcc tgcgccggac gttgaccttg
    13981 acggtccgtt ccagatgcag atttctcagc tcgattacaa cagctatgtt ggcgttatcg
    14041 gcattggccg catcaagcgc ggtaaagtga agccgaacca gcaggtcact atcatcgata
    14101 gcgaaggcaa aacccgcaac gcgaaagtcg gtaaagtgct gggccacctc ggtctggaac
    14161 gtatcgaaac cgatctggcg gaagctggcg atatcgtggc gatcacgggc cttggcgaac
    14221 tgaacatttc tgacaccgtt tgcgacacgc aaaacgttga agcgctgccg gcactctccg
    14281 ttgatgagcc gaccgtttct atgttcttct gcgttaacac ctcgccgttc tgcggtaaag
    14341 aaggtaagtt cgtaacgtct cgtcagatcc tggatcgtct gaacaaagaa ctggtacaca
    14401 acgttgcgct gcgcgtagaa gaaaccgaag acgccgatgc gttccgcgtt tctggtcgtg
    14461 gcgaactgca cctgtctgtt ctgatcgaaa acatgcgtcg tgaaggtttc gaactggcgg
    14521 tatcccgtcc aaaagtaatc ttccgtgaaa tcgacggtcg taaacaagag ccgtatgaaa
    14581 acgtgactct ggacgttgaa gaacagcatc agggttctgt aatgcaggcg ctgggcgaac
    14641 gtaaaggcga cctgaaaaac atgaatccag acggtaaagg ccgcgtacgt ctcgactacg
    14701 tgatcccaag ccgtggtctg attggcttcc gttctgagtt catgaccatg acttccggta
    14761 ctggtctgct gtactccacc ttcagccact acgacgacgt acgtccgggt gaagtgggtc
    14821 agcgtcagaa cggcgtactg atctctaacg gtcagggtaa agcggtcgcg ttcgcactgt
    14881 tcggtctgca ggaccgcggt aagctgttcc tcggtcacgg tgcagaagtt tacgaaggtc
    14941 agattatcgg tattcacagc cgctctaacg acctgactgt aaactgcctg accggtaaga
    15001 aactgaccaa catgcgtgct tccggtactg acgaagctgt tgttctggtt ccgcctatcc
    15061 gcatgactct ggaacaagct ctggagttca tcgatgatga cgaactggta gaagtgactc
    15121 cgacctctat ccgtattcgt aaacgtcacc tgacggaaaa cgatcgtcgt cgcgccaacc
    15181 gcgcaccgaa agacgattaa tttcgttttt tagttataaa aaacctgcca tcgct
//
LOCUS       DAJTZL010000098        15084 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.098, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000098 DAJTZL010000000
VERSION     DAJTZL010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15084
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.098"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..781
                     /locus_tag="OO544_002614"
     CDS             <1..781
                     /locus_tag="OO544_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6768180.1"
                     /translation="NKESLFFMSVMELRDLLIALESRLHQKHKLNAAARLQYEQARNK
                     VIKKMAENIPEILVDELKNADINRRVNTLELTDNQGENLTFVLTLHDGSKCELVVNEL
                     QIEMLARAIIHAINNAEMRELALRITSLLDFLPLYDVDCQDNGNLEYDTYSQPEWKHN
                     LFDHYLAVLYRFKDESGKEQFSGTVVKTREATPGKEIEAITRRMLDFSPRLKKLAGVP
                     CQVYVRTVAANNAQPLTQDQCLRALHHLRVQSTSKTAPQAK"
     gene            complement(984..1337)
                     /locus_tag="OO544_002615"
     CDS             complement(984..1337)
                     /locus_tag="OO544_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HCR6768181.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(1475..3121)
                     /gene="groL"
                     /locus_tag="OO544_002616"
     CDS             complement(1475..3121)
                     /gene="groL"
                     /locus_tag="OO544_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HCR6768182.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(3165..3458)
                     /locus_tag="OO544_002617"
     CDS             complement(3165..3458)
                     /locus_tag="OO544_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HCR6768183.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            3734..4990
                     /gene="yjeH"
                     /locus_tag="OO544_002618"
     CDS             3734..4990
                     /gene="yjeH"
                     /locus_tag="OO544_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HCR6768184.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAAQVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPANIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LTCFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLAGLWLFLPKRKTPENGITT"
     gene            complement(5012..5482)
                     /gene="fxsA"
                     /locus_tag="OO544_002619"
     CDS             complement(5012..5482)
                     /gene="fxsA"
                     /locus_tag="OO544_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HCR6768185.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDR"
     gene            5819..7255
                     /gene="aspA"
                     /locus_tag="OO544_002620"
     CDS             5819..7255
                     /gene="aspA"
                     /locus_tag="OO544_002620"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HCR6768186.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            7373..8674
                     /gene="dcuA"
                     /locus_tag="OO544_002621"
     CDS             7373..8674
                     /gene="dcuA"
                     /locus_tag="OO544_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HCR6768187.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            8790..9128
                     /gene="cutA"
                     /locus_tag="OO544_002622"
     CDS             8790..9128
                     /gene="cutA"
                     /locus_tag="OO544_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HCR6768188.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            9104..10801
                     /gene="dsbD"
                     /locus_tag="OO544_002623"
     CDS             9104..10801
                     /gene="dsbD"
                     /locus_tag="OO544_002623"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HCR6768189.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSHFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVPQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTHLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGMLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPIFLLERVIGDIWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKKFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            10838..11413
                     /locus_tag="OO544_002624"
     CDS             10838..11413
                     /locus_tag="OO544_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR6768190.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            11520..11595
                     /locus_tag="OO544_002625"
     tRNA            11520..11595
                     /locus_tag="OO544_002625"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11553..11555,aa:Phe,seq:gaa)
     gene            12212..13749
                     /gene="cadC"
                     /locus_tag="OO544_002626"
                     /pseudo
     CDS             12212..13749
                     /gene="cadC"
                     /locus_tag="OO544_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313142.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylation/transport transcriptional
                     activator CadC"
     gene            14114..>15084
                     /gene="cadB"
                     /locus_tag="OO544_002627"
     CDS             14114..>15084
                     /gene="cadB"
                     /locus_tag="OO544_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HCR6768191.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLYLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLL"
BASE COUNT         3708 a         3764 c         3616 g         3996 t
ORIGIN      
        1 caataaagaa tcgttgttct ttatgtctgt catggaactg cgcgacctgc tgattgcgct
       61 ggaaagtcgt ctgcatcaaa agcataaact gaatgctgct gcacgccttc agtatgagca
      121 agctcgcaat aaagtcatta agaaaatggc agaaaacatc ccagaaattc tggttgatga
      181 gcttaaaaat gccgatatta atcgtcgggt taacacgctg gaacttaccg ataatcaagg
      241 cgaaaatctt accttcgtat taacgctaca tgacggtagc aaatgtgagt tagtcgtcaa
      301 tgagttgcag attgaaatgc tggcgcgagc aatcattcat gcaattaata atgcggaaat
      361 gcgtgaactg gcattacgta ttacctcact gttagatttc ctgccgttat acgacgttga
      421 ctgccaggac aatggcaatc tggaatatga cacctattcc caaccggaat ggaaacataa
      481 cctgttcgac cattatctgg cggtgcttta ccgctttaaa gatgaaagtg gcaaagaaca
      541 atttagcggc acagtggtga aaacgcgtga agcaacgccg ggtaaagaaa tagaggcgat
      601 cactcgccga atgcttgatt tcagcccacg actgaaaaaa ctcgcgggtg tgccatgtca
      661 ggtctatgtt cgcaccgtag cggcgaataa cgcccaaccg ctaacccagg atcaatgctt
      721 acgcgcgctg catcacttac gtgttcaatc caccagcaaa actgcaccac aggcgaaata
      781 atctcatctt tgctaatgcg aggctggagc catccagcct gaaatgactg ataacgtgat
      841 cgttggtcat gccggatgcg acgtaaacgc cttatccggc ctacaaattc tcaagcattc
      901 aatcagttgc aatgcctgct gtaggcctga taagcgaagc gcatcaggca ataatacgtt
      961 tgtcacccag cctctggatc acatcagttc gggcacttat aaacctgacc gataatctgg
     1021 ctatccgtcg ggacaaaact ggacaacatt ccctgcgacg ggctgctgat gccataaatc
     1081 acgttaccgc ccattgcagc cgcctggttg cgcagatcgt ttgctgcgcc gcgcatagaa
     1141 ccgccctctt ctccgtgttg cccggaaagc cagttgcttt gcttacctgt cgcagtacca
     1201 atcagctggc actctgcgcc tggctgctcg tccacaatgc gtacactctg accggcagca
     1261 ctcaattcgt tgctggagct acaacccgcc atcagtagcg cggcaccgac aatccctgct
     1321 aagtatttta cgtgcatgtt attccccata atcaatgagc tggacgccac tcgcgtcgtc
     1381 cgtgtctgaa tcttatacta gaaagatgac caaaagaaaa acccccagac atttctgccc
     1441 gggggtttgt ttatttctgc gaggtgcagg gcaattacat catgccgccc atgccaccca
     1501 tgccgcccat accgccagca gcgcctaagt cagctgcatc atttttcggc aggtcggtaa
     1561 ccatgcactc ggtggtgatc atcaggccag ccacagaagc cgcgtactgc agagcagaac
     1621 gggttacttt ggttgggtcc aggataccca tgtcgatcat gttgccgtat tcttcggttg
     1681 ctgcgttgta accgtagttg ccgtcgccgc ctttaacggt gttagcaaca acagacggtt
     1741 cttcgccgca gttcaggacg atctgacgca gcggagcttc cattgcacgc agtgcaactt
     1801 tgatacccac gttctggtct tcgttctgac cacgcaggtc agccagttta gacgctacgc
     1861 ggatcagcgc aacaccacca ccagcaacca cgccttcttc taccgcagca cgggtcgcgt
     1921 gcagggcgtc ttcaacgcgt gcttttttct ctttcatttc aacttcggta gcagcaccta
     1981 ctttgataac tgcaacgccg cctgccagtt tcgctacgcg ttcctgcagt ttttcacggt
     2041 cgtagtcaga agttgcttct tcaatctgct gacggatctg agcaacacgg ccctggattg
     2101 cagcttcttc acccacgcca tcgatgatag tggtggtgtc tttgttgatc acaacacgtt
     2161 tagcctgacc caggtcttcc agggttgctt tttccagttc cataccgatc tcttcagaga
     2221 tcacggtacc gccagtcagg gttgcgatat cctgcagcat agctttacga cgatcgccga
     2281 agcccggagc tttaactgca gcaactttca cgatgccacg catggtgtta acaaccagag
     2341 ttgccagcgc ttcgccttct acatcttcag cgatgatcag cagcggtttg cctgctttgg
     2401 caacggcttc cagaaccggc agcatttcgc ggatgttgga gattttcttg tcagccagca
     2461 ggatgaacgg gctttccagt tctactgcgc cagtttccgg cttgttgatg aagtaaggag
     2521 acaggtagcc acggtcgaac tgcatacctt caaccacgtc cagttcgtcc tgcagaccgg
     2581 taccgtcttc aacggtgata acgccttctt taccgacttt gtccatcgct tcagcgatca
     2641 gtttacctac ggtttcgtcg gagttagcgg agatggtacc aacctgagca atcgctttag
     2701 agtcagagca cggtacggac agcgctttca gttcttcaac tgcagcggta acggctttgt
     2761 cgataccacg tttcaggtcc atcgggttca tgcccgcagc aacagctttc agaccctcag
     2821 tgatgatagc ctgagccagt acagttgcag tggtggtacc gtcgcctgca gcgtcgttcg
     2881 ctttagaggc aacttctttc accatctgcg cacccatgtt ttcgaacttg tcttccagtt
     2941 cgatttcacg agcaacggaa acaccatctt tggtgatggt cggtgcaccg aaagatttat
     3001 ccagaactac gttacggcct ttcggaccga gggtaacttt cactgcatct gccagtacgt
     3061 ttacgccgcg cagcattttc acacgagcgt cgttaccgaa ttttacgtct ttagctgcca
     3121 ttatctttat tccttaaatt cgtatgttca gtgtcgtgcg aggattacgc ttcaacaatt
     3181 gccagaatgt cgctttcgga catgatcaac acttcttcat tgtcgatctt ctcagatttc
     3241 acaccgtagc catcgttgaa aataacgatg tcgccaactt tcacatccag cggcttcact
     3301 tcgccatttt caaggatacg gccattgccg acagccagca cttcgccgcg ggtggattta
     3361 gccgctgcag agccggtcag aacgatgccg ccagcagatt tagtttcaac ttctttacgc
     3421 ttgacgatca cgcgatcatg caatggacga atattcattg ataactctcc tttgagaaag
     3481 tccgtatctg ttatgggtga cgccggagct tacgtggttt cccggctggt gaccagagaa
     3541 atggggatga ggattcgccc cttcaagggg gaaaaagaaa aaaaattctg atcagcacaa
     3601 aatcgggtga aaaccctgat tcacctcaca tttcatcgca atttcttcat cgccgtcata
     3661 agcgaatctg attgtgctac catcgaaaat ctacgcagtt gcccaaaatt tgggcgcaat
     3721 cggatatcaa cccatgagtg gactcaaaca agaactgggg ctggcccagg gcattggcct
     3781 gctatcgacg tcattattag gcaccggcgt gtttgccgtt cctgcgttag ctgcacaggt
     3841 agcaggcaat aacagcctgt gggcgtggcc cgttttgatt atcttagtgt tcccgattgc
     3901 gattgtgttt gcgattctgg gtcgccacta tcccagcgca ggcggtgtcg cgcacttcgt
     3961 cggtatggcg tttggttcgc ggcttgagcg agtcaccggc tggttgtttt tatcggtcat
     4021 tcccgtgggt ttgcctgccg cgctacaaat tgccgccggg ttcggccagg caatgtttgg
     4081 ctggcatagc tggcaactgt tgttggcaga actcggtacg ctggcgctgg tgtggtatat
     4141 cggtactcgc ggtgccagtt ccagtgctaa tctacaaacc gttattgccg gacttatcgt
     4201 cgcgctgatt gtcgctatct ggtgggcggg cgatatcaaa cctgcgaata tccccttccc
     4261 ggcacctggt aatatcgaac ttaccgggtt atttgctgcg ttatcagtga tgttctggtg
     4321 ttttgtcggt ctggaagcat ttgcccatct tgcctcggaa tttaaaaatc cagagcgtga
     4381 ttttcctcgc gcattgatga ttggtctgct gctggcagga ttagtctact ggggctgtac
     4441 ggtagtcgtc ttacacttcg acgcctatgg tgaacaaatg gccgcggcag catcgcttcc
     4501 aaaaattgta gtgcaactgt tcggtgtagg agcgttatgg attgcctgcg taattggcta
     4561 tctgacctgc tttgccagtc tcaacattta tatacagagc ttcgcccgcc tggtctggtc
     4621 gcaggcgcaa cataatcctg accactacct ggcacgcctc tcttctcgcc atatcccgaa
     4681 taatgccctc aatgcggtgc tcggctgctg cgtggtgagc actttggtga ttcatgcttt
     4741 agagatcaat ctggacgctc ttattattta tgccaatggc atctttatta tgatttatct
     4801 gttatgcatg ctggcaggct gtaaattatt gcaaggacgt tatcgactac tggcggtggt
     4861 tggcgggctg ttatgcgttc tgttactggc aatggtcggc tggaaaagtc tctatgcgct
     4921 gattatgctg gcggggttat ggctgtttct gccaaaacga aaaacgccgg aaaatggcat
     4981 aaccacataa tccggcgttt cgacattaat cctagcgatc gtctttatga tcaaggcggt
     5041 cgcggtcatc atcctttcgc tggtactcac catcaaaagt attaccgcca ccggtcccgg
     5101 cgctaaaacc gccgcctggc atgcgagaaa agcgcaaatg tggcatcaac ttcactgtca
     5161 gatgcttttg caccggcggc aataaaagca gaagaccgag gaagtcggtg aaaaagcccg
     5221 gtaataaaag cagcaaaccg gcaataatca gcgaaacact tttaatcatc tccgccgcag
     5281 ggttttcacc cgccgccatt ttttgctgca tcagcacgaa attcttaaag ccctggttac
     5341 gtaccagtga cataccgata actgacgtga atataaccag cacgagggtc agcaataccc
     5401 ccaatacatg ggcaacctga ataaagattg aaatctcaat atagacataa aggaaaatgg
     5461 caataaaagg taaccagcgc aaaggtttct cctgtaatag cagccggtta aacccggcta
     5521 cctgaatggg ttgcgaatcg cgttcagctt atattgtggt cattagcaaa atttcaagat
     5581 gtttgcgcaa ctatttttgg tagtaatccc aaagcggtga tctatttcac aaattaataa
     5641 ttaaggggta aaaaccgaca cttaaagtga tccagattac ggtagaaatc ctcaagcagc
     5701 atatgatctc gggtattcgg tcgatgcagg ggataatcgt cggtcgaaaa acattcgaaa
     5761 ccacatatat tctgtgtgtt taaagcaaat cattggcagc ttgaaaaaga aggttcacat
     5821 gtcaaacaac attcgtatcg aagaagatct gttgggtacc agggaagttc cagctgatgc
     5881 ctactatggt gttcacactc tgagagcgat tgaaaacttc tatatcagca acaacaaaat
     5941 cagtgatatt cctgaatttg ttcgcggtat ggtaatggtt aaaaaagccg cagctatggc
     6001 aaacaaagag ctgcaaacca ttcctaaaag tgtagcgaat gccatcattg ccgcatgtga
     6061 tgaagtcctg aacaacggaa aatgcatgga tcagttcccg gtagacgtct accagggcgg
     6121 cgcaggtact tccgtaaaca tgaacaccaa cgaagtgctg gccaatatcg gtctggaact
     6181 gatgggtcac cagaaaggtg aatatcagta cctgaacccg aacgaccatg ttaacaaatg
     6241 tcagtccact aacgacgcct acccgaccgg tttccgtatc gcagtttact cttccctgat
     6301 taagctggta gatgcgatta accaactgcg tgaaggcttt gaacgtaaag ctgtcgaatt
     6361 ccaggacatc ctgaaaatgg gtcgtaccca gctgcaggac gcagtaccga tgaccctcgg
     6421 tcaggaattc cgcgctttca gcatcctgct gaaagaagaa gtgaaaaaca tccaacgtac
     6481 cgctgaactg ctgctggaag ttaaccttgg cgcaacagca atcggtactg gtctgaacac
     6541 gccgaaagag tactctccgc tggcagtgaa aaaactggct gaagtcactg gcttcccatg
     6601 cgtaccggct gaagacctga tcgaagcgac ctctgactgc ggcgcttatg ttatggttca
     6661 cggcgcgctg aaacgcctgg ctgtgaagat gtccaaaatc tgtaacgacc tgcgcttgct
     6721 ctcttctggt ccacgtgccg gcctgaacga gatcaacctg ccggaactgc aggcgggctc
     6781 ttccatcatg ccagctaaag taaacccggt tgttccggaa gtggttaacc aggtatgctt
     6841 caaagtcatc ggtaacgaca ccactgttac catggcagca gaagcaggtc agctgcagtt
     6901 gaacgttatg gagccggtca ttggccaggc tatgttcgaa tccgttcaca ttctgaccaa
     6961 cgcttgctac aacctgctgg aaaaatgcat taacggcatc actgctaaca aagaagtgtg
     7021 cgaaggttac gtttacaact ctatcggtat cgttacttac ctgaacccgt tcatcggtca
     7081 ccacaacggt gacatcgtgg gtaaaatctg tgccgaaacc ggtaagagtg tacgtgaagt
     7141 cgttctggaa cgcggtctgt tgactgaagc ggaacttgac gatattttct ccgtacagaa
     7201 tctgatgcac ccggcttaca aagcaaaacg ctatactgat gaaagcgaac agtaatcgta
     7261 cagggtagta caaataaaga aggcacgtca gatgacgtgc cttttttctt gtgagcagta
     7321 acttaaaaat aacaatctaa tatcaacttg ttaaaaaaca aggaaggcta atatgctagt
     7381 tgtagaactc atcatagttt tgctggcgat cttcttgggc gccagattgg ggggaatagg
     7441 tattggtttt gcaggcggat tgggggtgct ggttcttgcc gctattggcg ttaaacccgg
     7501 taacatcccg ttcgatgtca tttccattat catggcggtt atcgccgcta tttctgccat
     7561 gcaggttgct ggcggtctgg actatctggt tcatcagaca gaaaagctgc tgcgccgtaa
     7621 cccgaaatac atcacaatcc tcgcaccgat cgtgacctat ttcctgacaa tctttgctgg
     7681 tactggcaac atctctctgg cgacactgcc agttatcgct gaagttgcga aggaacaagg
     7741 cgttaaacct tgccgtccgc tgtctactgc agtggtatcc gcgcagattg cgatcaccgc
     7801 atcgccaatc tcagcggcag tggtttacat gtcttccgtg atggaaggtc atggcatcag
     7861 ctacctccat ctgctctccg tggtcatccc gtccaccctg ctggcggttc tggtgatgtc
     7921 cttcctggtc actatgctgt tcaactccaa actctctgac gatccgattt atcgcaagcg
     7981 tctggaagag ggcctggttg aactgcgcgg tgaaaagcag attgaaatca aatccggtgc
     8041 aaaaacgtcc gtctggctgt tcctgctggg cgtagttggc gtggttatct atgcaatcat
     8101 caacagccca agcatgggtc tggttgaaaa accgctgatg aacaccacca acgcaatcct
     8161 gatcatcatg ctcagcgttg caactctgac caccgttatc tgtaaagtcg ataccgacaa
     8221 catcctcaac tccagcacct tcaaagcagg tatgagcgcc tgtatttgta tcctgggtgt
     8281 tgcgtggctg ggcgatactt tcgtttccaa caacatcgac tggatcaaag ataccgctgg
     8341 tgaagtgatt cagggtcatc cgtggctgct ggccgtcatc ttcttctttg cttctgctct
     8401 gctgtactct caggctgcaa ccgcaaaagc actgatgccg atggctctgg cactgaacgt
     8461 ttctccgctg accgctgttg cttctttcgc tgcggtgtct ggtctgttca ttctgccgac
     8521 ctatccgacg ctggttgctg cggtacagat ggatgacacg ggtacaaccc gtatcggtaa
     8581 attcgtcttc aaccatccgt tcttcatccc gggtactctg ggtgttgccc tggccgtttg
     8641 cttcggcttc gtgctgggta gcttcatgct ataatgacca atcgcggggc gttcacgccc
     8701 cgctttcttt cccgccgact aacatccttc ccccgtccgt tgtatagtga cctctctctt
     8761 gcggttccat ctgttcttgc gaggtgttta tgcttgatga aaaaagttcg aataccgcgt
     8821 ctgtcgtggt gctatgtacc gcaccggatg aagcgacagc ccaggattta gccgccaaag
     8881 tgctggcgga aaaactggcg gcctgcgcga ccttgatccc cggcgctacc tctctctatt
     8941 actgggaagg taagctggag caagaatacg aagtgcagat gattttaaaa actaccgtat
     9001 ctcaccagca ggcactgctg gaatgcctga agtctcatca tccatatcaa accccggaac
     9061 ttctggtttt acctgttaca cacggagaca cagattacct ctcatggctc aacgcatctt
     9121 tacgctgatc ctgctacttt gcagcacttc cgtttttgcc ggattattcg acgcgccggg
     9181 acgttcacat tttgtccccg cggatcaagc ctttgctttt gattttcagc aaaaccaaca
     9241 tgacctgaat ctgacctggc agatcaaaga cggttactac ctctaccgta aacagatccg
     9301 cattacgccg gaacacgcga aaattgccga cgtgcagctg ccgcaaggcg tctggcatga
     9361 agatgagttt tacggcaaaa gcgagattta ccgcgatcgg ctgacgcttc ccgtaaccat
     9421 caaccaggcg agtgcgggag cgacgttaac tgtcacctac cagggctgtg ctgatgccgg
     9481 tttctgttat ccgccagaaa ccaaaaccgt tccgttaagc gaagtggtcg ccaacaacgc
     9541 agcgtcacag cctgtgtctg ttccgcagca agagcagcac accgcgcaat tgcccttttc
     9601 cgcgctctgg gcgttgttga tcggtattgg tatcgccttt acgccatgcg tgctgccaat
     9661 gtacccactg atttctggca tcgtgctggg tggtaaacag cggctctcca ctgccagagc
     9721 attgttgctg acctttattt atgtgcaggg gatggcactg acttacacgg cgctgggtct
     9781 ggtagttgcc gccgcagggt tacagttcca ggcggcgcta cagcacccat acgtgctcat
     9841 tggcctcgcc atcgtcttta ccttgctggc gatgtcaatg tttggcttgt ttactctaca
     9901 actcccctct tcgctgcaaa cacatctcac attgatgagc aatcgccaac agggcggctc
     9961 acctggcggt gtgtttgtta tgggggcgat tgccggactg atctgttcac catgcaccac
    10021 cgcaccgctt agcgcgattc tgctgtatat cgcccaaagc gggaacatgt ggctgggcgg
    10081 cggcatgctt tatctctatg cgttgggcat gggcctgccg ctgatgctaa ttaccgtctt
    10141 tggtaaccgc ttgctgccga aaagcggccc gtggatggaa caagtcaaaa ccgcgtttgg
    10201 ttttgtgatc ctcgcactgc cgatcttcct gctggagcga gtgattggtg atatatgggg
    10261 attacgcttg tggtcggcgc tgggtgtcgc attctttggc tgggccttta tcaccagcct
    10321 acaggccaaa cgcggctgga tgcgcgtggt gcaaataatc ctgctggcag cggcattggt
    10381 tagcgtgcgc ccacttcagg attgggcatt tggtgcgacg cataccgcgc aaactcagac
    10441 gcatctcaac tttacacaaa tcaaaacggt agatgagtta aatcaggcgc tcgttgaagc
    10501 caaaggcaaa ccggtgatgt tagatcttta tgccgactgg tgcgtcgcct gtaaaaagtt
    10561 tgagaaatac accttcagcg acccgcaggt gcaaaaagcg ttagcagaca cggtcttact
    10621 tcaggccaac gtcactgcta acgacgcaca agatgtggcg ctgttaaagc atcttaatgt
    10681 ccttggacta ccgacaattc tcttttttga cggacaaggc caggagcatc cacaagcacg
    10741 cgtcacgggc tttatggatg ctgaaacctt cagcgcacat ttgcgcgatc gccaaccgtg
    10801 aacgacactt gcagtgggaa agacggagga aaatagcgtg caacgtgaag atgtactggg
    10861 agaagccctg aaattattag aattacaagg gattgccaac accacgctgg agatggttgc
    10921 tgaacgtgtg gattatccac tggacgagct acgccgcttc tggccagaca aagaggcaat
    10981 cctctacgat gcgctgcgtt atcttagcca acagatagat gtctggcgtc gtcagctgat
    11041 gcttgacgaa acgcaaaccg ccgaacaaaa gctgctggca cgttatcagg cgttatcgga
    11101 gtgcgttaaa aacaaccgct atccgggctg tctatttatc gctgcctgta cgttttatcc
    11161 cgaccctggc caccctattc atcaactggc cgatcagcaa aaaagcgcgg cctacgattt
    11221 cacccacgaa ctgttaacca cgctggaagt tgacgatccg gcgatggtag caaagcagat
    11281 ggaactggtg ctggaaggct gtttaagccg aatgctggtg aatcgtagcc aggcggatgt
    11341 cgacaccgca catcggctgg cggaagatat cctgcgcttc gcccgctgcc gccagggtgg
    11401 tgcactgacc tgacagaaac acagaaaaga agcgatttgc cgcaatctta agcagttgaa
    11461 tcgcttttac tgaaattagg ttgacgagat gtgcagatta cggtttaatg cgccccgttg
    11521 cccggatagc tcagtcggta gagcagggga ttgaaaatcc ccgtgtcctt ggttcgattc
    11581 cgagtccggg caccaaattc atataaacgg acctccacgg aggtccgttt ttcgtttcag
    11641 gacgccacga tttaagcgtt ctgcctccaa atcaattcta ccgaactcaa ccagattctc
    11701 cccacatcac cagcattttg cgggtatatc ccaattcggg aaaatttgtt cctgagcgat
    11761 agcactgact gacgtgaaat gtcgtgcggc cccgtgatgt tgttgaacgt caaatgacgt
    11821 catcaggagc gtaacgcatc cataaagcac aacaccggtc tgaacggcga ctgatgagat
    11881 tttctgaatg agaacaaaga gaaatgtatc agtccgcttg ctcatgcaaa gactaacaat
    11941 ccattaaaat agtaagcgct ccggacaatt ttccatggat tattttctac acatttttct
    12001 ttggcaaaga tgatgaattt tgatggtaag gaatattact tctggttctc agtaaaatcc
    12061 tttcgtaata ctatgtaatc aagaagttta tggctggtaa aaataacgtc ttgcattcac
    12121 caataacatg taaataaacc catctattga tggtaaaaat aggtcatggc aattatcatt
    12181 gcaccattcc cttttcgaat gagtttctat tatgcaacaa cctgtagttc gcgttggcga
    12241 atggcttgtt actccgtcca taaaccaaat tagccgcaat gggcgtcaac ttacccttga
    12301 gccgagatta attgatcttc tggttttctt tgctcaacac agtggcgaag tacttagcag
    12361 ggatgaactt atcgataatg tctggaagag aagtattgtc accaatcacg ttgtgacgca
    12421 gagtatctca gaactacgta agtcattaaa agataatgat gaagatagtc ctgtctatat
    12481 cgctactgta ccaaagcgcg gctataaatg aatggtgccg gttatctggt acagcgaaga
    12541 agagggagag gaaataatgc tatcttcgcc tccccctata ccagaggcgg ttcctgacac
    12601 aaattctccc tcccacagtc ttaccattca aaacaccgca acgccacctg aacaatcccc
    12661 agttaaaagc aaacgattca ctaccttttg ggtatggttt ttttcctgtt gtcgttaggt
    12721 atctgtgtag cactggtagc gttttcaact cttgatacac gtcttcctat gagcaaatcg
    12781 cgtattttgc tcaatccacg cgatattgac attaatatgg taaataaaag ttgtaacagc
    12841 tggagttccc cgtatcagct ctcttacgcg ataggcgtgg gtgatttggt ggcgacatca
    12901 cttaacacct tctccacctt tatggtgcat gacaaaatca actacaacat tgatgaaccg
    12961 agcagttccg gtaaaacatt atctattgcc tttgttaatc agcgccaata ccgtgctcaa
    13021 caatgcttta tgtcggtaaa attagtagac aatgcagatg gttcaaccat gctggataaa
    13081 cgttatgtca tcactaacgg taatcagctg gcgattcaaa atgatttact ggagagttta
    13141 tcaaaagcgt taaaccaacc gtggccacaa cgaatgcagg agacgctcca gcaaattttg
    13201 cctcatcgtg gtgcgttatt aactaatttt tatcaggctc atgattattt actgcatggc
    13261 gatgacaaat cattgaatcg tgccagtgaa ttattaggtg agattgttca atcatcccca
    13321 gaatttacct acgcgagagc agaaaaagca ttagttgata tcgtgcgcca ttctcaacac
    13381 cctttagatg aaaaacaatt agcagcactg aacacagaaa tagacaacat tgttacactg
    13441 ccggaattga acaacctgtc cattatatat caaataaaag cggtcagtgc tctggtaaaa
    13501 ggtaaaatag atgagtctta ccaggcgata aatactggca ttgatcttga aatgtcctgg
    13561 ctaaattatg tgttgcttgg caaggtttat gaaatgaagg ggatgaaccg ggaagcagct
    13621 gatgcatatc tcaccgcctt taatttacgc ccaggggcaa acacccttta ctggattgaa
    13681 aatggtattt tccagacttc tgttccttat gttgtacctt atctcgacaa atttctcgct
    13741 tcagaataag taactccggg ttgatttatg ctctggaata tttgttgttg agtttttgta
    13801 tgttcccatt ggtataatat gttgcggcaa tttatttgcc gcataatttt tattacataa
    13861 atttaaccag agaatgtcat gcaatccatt gtaaacattg aatgtttatc ttttcatgat
    13921 atcaacttgc gatcctgatg tgttaataaa aaacctcaag ttctcactta cagaaacttt
    13981 tgtgttattt cacctaatct ttaggattaa tccttttttc gtgagtaatc ttatcgccag
    14041 tttggtctgg tcaggaaata gttatacatc atgacccgga ctccaaattc aaaaatgaaa
    14101 ttaggagaag agcatgagtt ctgccaagaa gatcgggcta tttgcctgta ccggtgttgt
    14161 tgccggtaat atgatgggga gcggtattgc attattacct gcgaacctag caagtatcgg
    14221 tggtattgct atctggggtt ggattatctc tattattggt gcaatgtcgc tggcgtatgt
    14281 atatgcccga ctggcaacaa aaaacccgca acaaggtggc ccaattgctt atgccggaga
    14341 aatttctcct gcatttggtt ttcagacagg tgttctttat taccatgcta actggattgg
    14401 taacctagcg attggtatta ccgctgtatc ttatctttcc accttcttcc cagtattaaa
    14461 tgatcctgtt ccggcgggta tcgcctgtat tgctatcgtc tgggtattta cctttgtaaa
    14521 tatgctcggc ggtacctggg taagccgttt aaccactatt ggtctggtgc tggttcttat
    14581 tcctgtggtg atgactgcta ttgttggctg gcattggttt gatgcggcaa cttatgcagc
    14641 taactggaat actgcggata ccactgatgg tcatgcgatc attaaaagta ttctgctcta
    14701 cctgtgggcc ttcgtgggtg ttgagtccgc agcagtaagt actggtatgg ttaaaaaccc
    14761 gaaacgtacc gttccgctgg caaccatgct gggtactggt ttagcaggta ttgtttacat
    14821 cgctgcgact caggtgcttt ccggtatgta tccgtcttct gtaatggcgg cttccggtgc
    14881 tccgtttgca atcagtgctt caactatcct cggtaactgg gctgcgccgc tggtttctgc
    14941 attcaccgcc tttgcgtgcc tgacttctct gggctcctgg atgatgttgg taggccaggc
    15001 aggtgtacgt gccgctaacg acggtaactt cccgaaagtt tatggtgaag tcgacagcaa
    15061 cggtattccg aaaaaaggtc tgct
//
LOCUS       DAJTZL010000099        14965 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.099, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000099 DAJTZL010000000
VERSION     DAJTZL010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14965
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.099"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            313..1611
                     /gene="kgtP"
                     /locus_tag="OO544_002628"
     CDS             313..1611
                     /gene="kgtP"
                     /locus_tag="OO544_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HCR6768192.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HVNVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1608..1931)
                     /locus_tag="OO544_002629"
     CDS             complement(1608..1931)
                     /locus_tag="OO544_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HCR6768193.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(1977..3332)
                     /gene="pssA"
                     /locus_tag="OO544_002630"
     CDS             complement(1977..3332)
                     /gene="pssA"
                     /locus_tag="OO544_002630"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HCR6768194.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENSGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLKLAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3446..6106)
                     /gene="pat"
                     /locus_tag="OO544_002631"
     CDS             complement(3446..6106)
                     /gene="pat"
                     /locus_tag="OO544_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HCR6768195.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGSFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AVSNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPTLPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            complement(6138..6836)
                     /gene="tapT"
                     /locus_tag="OO544_002632"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6138..6836)
                     /gene="tapT"
                     /locus_tag="OO544_002632"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HCR6768196.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVHRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6905..7324)
                     /gene="trxC"
                     /locus_tag="OO544_002633"
     CDS             complement(6905..7324)
                     /gene="trxC"
                     /locus_tag="OO544_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HCR6768197.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7531..8568
                     /locus_tag="OO544_002634"
     CDS             7531..8568
                     /locus_tag="OO544_002634"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HCR6768198.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8616..9305)
                     /gene="ung"
                     /locus_tag="OO544_002635"
     CDS             complement(8616..9305)
                     /gene="ung"
                     /locus_tag="OO544_002635"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HCR6768199.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAESE"
     gene            9610..9993
                     /gene="grcA"
                     /locus_tag="OO544_002636"
     CDS             9610..9993
                     /gene="grcA"
                     /locus_tag="OO544_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HCR6768200.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10049..10636)
                     /gene="eamB"
                     /locus_tag="OO544_002637"
     CDS             complement(10049..10636)
                     /gene="eamB"
                     /locus_tag="OO544_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HCR6768201.1"
                     /translation="MTPTLLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10739..11620
                     /locus_tag="OO544_002638"
     CDS             10739..11620
                     /locus_tag="OO544_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6768202.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIVDVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPRFAVAKELECGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHTFIQCVEESFVAG"
     gene            complement(11829..13163)
                     /gene="srmB"
                     /locus_tag="OO544_002639"
     CDS             complement(11829..13163)
                     /gene="srmB"
                     /locus_tag="OO544_002639"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HCR6768203.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            13295..14032
                     /gene="trmN"
                     /locus_tag="OO544_002640"
     CDS             13295..14032
                     /gene="trmN"
                     /locus_tag="OO544_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HCR6768204.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWLTQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQVGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(14017..>14965)
                     /locus_tag="OO544_002641"
     CDS             complement(14017..>14965)
                     /locus_tag="OO544_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HCR6768205.1"
                     /translation="PLLPVHLSRVANLEFNQFPPTALYHPQARNFLLTEALRGEGAYL
                     KRPDGTRFMPDFDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPM
                     IYEKLLGLGIDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGAN
                     RMASNSLLECLVYGWSAAEDITRRMPDAHGVSTLPPWDESRVENPDERVVIQHNWHEL
                     RLFMWDYVGIVRTTKRLERALRRINMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIV
                     RCAMMRKESRGLHFTLDYPELLTHSGPSILPPGNHYINR"
BASE COUNT         3612 a         3784 c         3922 g         3647 t
ORIGIN      
        1 aaaggccatc ctgacggatg gcctttttgc attggcgcag aaaaaaatgc ctgatgcgac
       61 actgcgcgtc ttatactccc acatatgcca gattcagcaa cggatacggt ttccccaact
      121 tgcccacttc catacgtgtc ctccttacca gaaatttatc cttaagctcc tcaataacca
      181 ttttcctgct aactaaattc atggttaagg ttgcacaatg atatgcaaca aatgtataac
      241 attcccttta ccaaaaaaaa taaacaaaag cgaccgacaa aagcatcgga ttacggcagg
      301 agacataatg gcatggctga aagtactgta acggcagaca gcaaactgac aagtagtgat
      361 actcgtcgcc gcatttgggc aattgtgggg gcctcttcag gtaatctggt cgagtggttc
      421 gatttctatg tctactcgtt ctgttcactc tacttcgccc acatcttctt cccttccggg
      481 aacacgacga ctcaactact acaaacagca ggtgtttttg ctgcgggatt cctgatgcgc
      541 ccaataggcg gttggctatt tggccgcata gccgataaac atggtcgcaa aaaatcgatg
      601 ctgctatcgg tgtgtatgat gtgtttcggc tcactggtaa tcgcctgttt gcctggttat
      661 gaaacaatcg gtacgtgggc tccggcatta ttgcttctcg cgcgtttatt tcagggatta
      721 tccgttggcg gagaatatgg caccagcgcc acctatatga gtgaagttgc cgttgaaggg
      781 cgcaaaggtt tttacgcatc atttcagtat gtgacgttga tcggcggaca actgctagcc
      841 ctactggttg tcgtggtttt acaacacacc atggaagacg ctgcactcag agagtgggga
      901 tggcgtattc ctttcgcgtt aggagctgtg ttagctgttg tggcgttgtg gttacgtcgt
      961 cagttagatg aaacttcgca acaagaaacg cgcgctttaa aagaagctgg atctctgaaa
     1021 ggattatggc gcaatcgccg tgcattcatc atggttctcg gttttaccgc tgcgggctcc
     1081 ctttgtttct ataccttcac tacttatatg cagaagtatc tggtaaatac tgcgggaatg
     1141 catgtcaacg tggcgagtgg cattatgact gccgcattgt ttgtattcat gcttattcaa
     1201 ccactcattg gcgcgctgtc ggataagatt ggtcgccgta cctcaatgtt atgtttcggt
     1261 tcgctggcag ccatttttac cgttcctatt ctctcagcat tgcaaaacgt ttcctcgcct
     1321 tatgccgctt ttggtctggt gatgtgtgcc ctgctgatag tgagttttta tacatcaatc
     1381 agtggaatac tgaaggctga gatgttcccg gcacaggttc gcgcattagg cgttggcctg
     1441 tcatatgcgg tcgctaatgc tatatttggt ggttcggcgg agtacgtagc gttgtcgctg
     1501 aaatcaatag gaatggaaac agccttcttc tggtatgtga ccttgatggc cgtggtggcg
     1561 tttctggttt ctttgatgct acatcgcaaa gggaagggga tgcgtcttta gtgacgggtc
     1621 agttgccaga cggtatagcc ggtgcttgca ccggcgacat cccaggccaa atccttccag
     1681 ctccagccgc tcccttcggg gcggctatcc caaagctctt ttgacgcccc caaactgaca
     1741 gagaacatca atccaaacat ggcactgcga tcccggctca tcccctgatg ctgtgaatat
     1801 tcatttccgg cggcggaaag catcgccgag gcgataaagt gttgagcttt atcctgcccg
     1861 ctccagctat cgttagccat atggctgcat ccagaaagta acaatagcga acagacaaaa
     1921 agaatacgca aaacgtggcc tccaaaaaac aaaccccgta caggacgggg ttgtgattac
     1981 aggatgcggc taattaatcg gtcgatgcgg atacggcgca aacggcggat gagtttacga
     2041 accttcaccg gataatcggc aatactttgc agatcgcgat agtgcttaac gatggtggta
     2101 tgctcgcgga tcagctccag ttctttctct cgctgcggcg caagcttcag ttgtggatcg
     2161 tggatcaaaa tggcgttttc cagatccaga cgccaggcgc gcgggttcag gttattaccg
     2221 gtgatcaaca tccacttatc atcaacccac atccctttca ggtgataggt gttgtcgtca
     2281 tctttccata accgaaccac tagctggtca gtattgacgt aatactgcaa acggctcagg
     2341 aaacgacgca gattgatctc atagagataa ggcaatgcgc caattatctt gaaaggttca
     2401 tcttccggaa tgtagaagtc gttcgcggtt ttatcaccaa caataatttc gacctttttc
     2461 ccttcgcgca gcaactggat aatattgcgc acaaggattg ctggcaggtt gaagtatggc
     2521 gtacagatgg ttagtttctg ctcggcacaa ggcataagat ggaaaatggt cttgttcaac
     2581 agactcgatt tccccagccc cactagcggc gttacggaaa gctgatcgtt gtcggcatcg
     2641 ccctggaaat gataagcggc atcacgcagc tcctggcgga acagacgaat atcgttcttg
     2701 atttccgggc tttttggccg attaacatca tccagacgat taacgccgcg gccattcata
     2761 atattctgtg taacccattc aaacataatg tctgacatct tacggttacg gataagatga
     2821 taacggtcgt agcgatattt atcgtgctga tgcaggtaaa catcgttcag gctggcaccg
     2881 ctataaagta cgctatcgtc gatgataaag cctttaaagt gcagaacacc aagggcttca
     2941 cgggtattga ttggaacgcc ataaaccgga acatctacgc ccgaattttc ctgcgccatg
     3001 cggcagtacc agtcagcgtt agtgttagat gccgcagcgc caatgcgtcc acgttgtgca
     3061 cgatgccagt cgaccagcac ccgcacatcc agttccggac gctgcctttt agcttcatac
     3121 aacgcgttca gaatgccttt gccaccgtca tcctgttcga gatacagggc gacaatgcaa
     3181 atgcgctgct tcgcgctggc tattttttcc agcagcgtct cccggaagtc ggcgggagcg
     3241 taaaagaaat cgacatcatc aactgattga gaaatcttgg gtagttgggc aaggtgttgt
     3301 tgatgtttat tacgcttaaa ttttgacaac atcacagtgc atttcttctc tgttcattga
     3361 agggtcctct gtgcaatgca gacgacataa gcgggcaata ataacaccag tcccgattaa
     3421 gtggtcaaca tttccagtac cttactcatg attcctcgcg ttgggcaaga tttagcgtaa
     3481 gcccaacgat cccctcttcg agctggatat caacgttaaa cccgagcttg cgggctagcg
     3541 ccaccatgcc acgattgttt ggcatcgtaa taccattcag acgttgtagt ccgtgatctc
     3601 gcgtataggt aatcaacttt tccattaagc gtcgacctaa gcctaaccct ttgagatccg
     3661 agcgaaccag cacagcaaat tcggcatcga tgttatcagg atcggaaatc gcacgcgtga
     3721 cgccgaggat ctcttccgtt tgatcaatac gtcgtaccgc tacaaacgcc atttcccgat
     3781 cgtagtcgat ctgtgtcatg ttggctaaat cttcatgggt aaattcgttg atctcgctaa
     3841 agtagcggta ataaagatct tctttggtga ctcgcgaaat gaattgctga agttgtggct
     3901 catcttctgg caaaatcggg cggaacaagc agcgttcacc gtttttcaat tctacccatt
     3961 cttccagctg atgcggataa gggcgcactg ccagccgact ctcgttatcg ccttcaaacg
     4021 gcgagatatc cagcgtgaca tccagcgcgg taaattcact gccagaagcc agcaaaggat
     4081 gaatatccag acgctgaatt tccgggcaat cgacaatcaa gttggaaacc tgcaccagaa
     4141 gctggctcaa gcctgcaaca tccaatgggc gtagcgcact gcgcgcacga atctttttac
     4201 ttttgatccc ctgaataacc agatagcggg ccaggttcat gttcagcggc ggcagtgcga
     4261 cgacggcttg atcttcagga cgccactcca caccgccttc acccagcatg atcaacggcc
     4321 cgaaaaccgg atcgtgctca accacaaccc gcaactcctg agcgccagca cggttagcca
     4381 tactttgcac caacaggccg tggacccgcg cctgtggcca ggccattttt acgcgatcga
     4441 aaatagcgtt cgccgcttgc tggacttcat tggctgtacg caggtaaagc atgacgccct
     4501 gaacttccga tttatgtgga atatccggcg aacgcaattt cagcgccacc ggataaccaa
     4561 tctgttcggc aatatgcacc gcttcggtgc tatcgctggc aatccaggta gggagcgtgt
     4621 tcatgccata cgcttgcagg atgggctgaa cttcatgggt atcgagcgac gtagcccctt
     4681 cggcaatcgc ctgttgcaac agaagatgcg cttctgcggt attggaagtc agattgctgg
     4741 gcaacgtcgg cgtttcgcgt agttgcttct gattacgccg gtactccacc atatgcataa
     4801 aagcagtgat ggttccttcc ggggtacggt aggtcggcag cccggcttcg ctgaataaac
     4861 gtcgtgcctc ttgcgaggag tgctcgccgc accagttcgt cagcaaagag acatatttgc
     4921 tgcggggatg atgctttacc gcttcaatta atacttgcgc gctttctgtt gcgggagcag
     4981 cggcgctggg cgaatgaata accatcagcg catcaaaatc ctggctgtgg agcagaatgt
     5041 ccagcgtttt gacataatgc tcgctgctgg cgtcatcgcg cagatcgaga gggttagaga
     5101 ctgccacatg ttctggcaat gcatcgcgca atttctggca ggtttcttcg cttagcgttg
     5161 ccagcttgcc attgcgtgac cataaggcat ccagcgccag cgcggcaggc gcagcaccgt
     5221 tgctgataat catcaaccgg tcgccacgca gcgggcgcat atggctaagg gtttccaccg
     5281 ccgaaaacag ttcgtgggta tcctgtaccc gcaacaaacc ggcacgctga atagccgcat
     5341 cccatgccgg gtccattcct gccgtcgtgt tgagcagtcg ctgtgccgcc gggctacgtc
     5401 cgcttttaat caccagaatc ggtttattac gcgaggcact acgggctgcc gaaacaaagc
     5461 gtcgcgcgtc gcttaactgt tcgagataga gcaggatggc gctggtttta ctgtcgcgcg
     5521 ccagatagtc gagcaattca tcaacgtcga tatccaggct gtcgccgagc gcaataaagt
     5581 aggaaaagcc catctcacgc tgttgcgccc agtcgaggat ggtgttggag acggctgccg
     5641 attgtgaaat aaacgccagc ttgccgcgtt taatcggcac aggcgaaaag ctggcattca
     5701 gaccttgcca gggagccagt aaacccagac tgtttggtcc aagcaggcgc atgttatggc
     5761 gcagggcgca ggcgcggaga tcttcgtgtt gcgatgccgg ggcggaaaga ataatgcagg
     5821 ttttacagcc tttctcgccg agctcttcca gaagagcaag attacggctg gcattggtac
     5881 ataaaaccgc aaggtcgggt gtaaagggca agctggcaat atccggccag gccaacacac
     5941 ccaacaccgc tttccaggct ggcgtcaccg ggagtaccgg tccgttaaag cttcccgcca
     6001 gcaggttacg catcatcagg taacctgcgc gattgggttt catcgacgcg ccaattaccg
     6061 ctatcgattt tggtcgcagt agtgcttcca gtcctcgctg actcataccg gtctcccgca
     6121 gaagtgaccg aatgatttta aacgctttct aactgttctg ctgtgatgct acccagatgt
     6181 tgcgtttttc ctgccagata gcgtgtttta aagcgggtaa aatgctcgcc taaccctgct
     6241 gccgccccgg tatcgccggc catatctaac agtgcgatgg ctacctcggc agtacaatat
     6301 tggcctgcag cctgggcttc acgcaggcga taggcagaaa gccgggaaag atcgacggaa
     6361 atgacgggaa gattatccag atacggactt ttacgaaaca tcttgcgagc ttccggccag
     6421 gtaccatcga gcatgataaa cagcggtggc ttaccggcag gtggtgtgaa gatcacttcc
     6481 cgttgctcat cagcatacga ggcgggaaag accaccattg gctgataatc cgggttttgt
     6541 accagatcca gcaaatcctg cgagggttcg gtacgcgacc attgaaacgc aacggtatca
     6601 ggcaaaatat cagcaatcag acgcccggta ttactgggct tcattggctc ggtgtcgaac
     6661 atcagcaaac agaagcgact ttttgcttgt gctggggtaa ttgtcgaaca gagacataat
     6721 ttctctggca aaagacagcg ttggcagcga tgaacgcgat taccgcgggc aagaaaagga
     6781 cgtgttgcgc gcgcaatacg ctcggcgcgt aactggagaa cagcgttttc ggtcataaga
     6841 gagcgtcgaa aaaacgccat tgtcgcagag gagaaaacgg ggcacaagat gcaccccggt
     6901 aagattaaag agattcgttc agccagctat cgaacggcgc tttcggtact gcgccattaa
     6961 gcatgtcgac aacctgaccg tttttgaaaa tcatgatcgt cggtatacta cgaattccaa
     7021 agcgactgct caattcacgt tcagcttcgg tattcacttt cacaaagcgc actttaccgc
     7081 tacgctcttg cgcgacatct tcaaaaattg gtgcgaaatt acggcagggg ccgcaccacg
     7141 gtgcccagaa gtcgatcacc acaggtagat catccttcag caatttgtcg agtgtttcac
     7201 cggtcgcatt aatcacctct ccgtcaaaca agtcgtgacc gcagcgtccg cattttgccg
     7261 catcttcgat ccgatcgtcg ggaatgcgat tgatggcctg acaatgggta caaacggtat
     7321 tcataactaa cctcgggatg agtaaggaga cttatcggca acgacgccgg tatgtttcta
     7381 atatgttaca tattatcggg gacattgttc cagacaacaa tctgttttat tcaataggta
     7441 tgatagtcgc aggctttggt acgaaatcat ggcgaaccgc gcggacttcg ggtaatctgc
     7501 gcgcttcgcg cagcgctggt ggagaaaagc atgaacgatg aaatgaaagg taaaagcggc
     7561 aaggtcaaag tgatgtatgt ccgcagtgat gatgattctg ataaacgtac ccacaacccg
     7621 cgtaccggga aagggggcgg gcgtccagga aaatctcgtg ctgacggtgg ccgtcgcccc
     7681 gcccgcgatg acaaacagag tcagccccgt gaccgcaagt gggaagattc gccgtggcgc
     7741 acggtttccc gcgcgccggg tgatgagacg ccggaaaagg ccgatcacgg tggcatcagt
     7801 ggtaaaagtt ttattgatcc ggaagtgttg cgtcgtcagc gtgcggaaga aacccgcgtc
     7861 tacggcgaaa atgcctgtca ggcactgttc cagagccgtc cggaagcaat tgttcgcgcc
     7921 tggtttatcc agagcgtaac gccacgtttt aaagaagcct tgcgctggat ggcggcaaac
     7981 cgcaaagcgt accatgtggt ggatgaagct gaattgacaa aagcgtcagg cacggaacat
     8041 cacggcggcg tttgcttcct gatcaaaaag cgtaacggta caaccgtgca gcagtgggta
     8101 agccaggcag gcgcgcagga ttgcgttctg gcactggaaa atgaatctaa cccgcataac
     8161 ctgggtggca tgatgcgcag ctgcgcgcac ttcggcgtga aaggtgtcgt ggtgcaggat
     8221 gcggcactgc tggagtcggg ggcggctatc cgtaccgcag aaggcggcgc agagcacgtt
     8281 cagccgatta ctggcgacaa cattgttaac gtgctggatg atttccgtca ggcaggttac
     8341 accgtagtga ccacttccag cgagcagggt aaaccgctgt tcaaaactag tctgccagcg
     8401 aaaatggtac tggtgctggg tcaggaatat gaagggttac cggatgccgc acgcgatccg
     8461 aacgatctgc gcgtgaagat tgacggtacg ggcaacgttg ccgggctgaa tatctctgtc
     8521 gcaaccggcg ttttgcttgg tgaatggtgg cgtcagaata aagcctgata aatcagccgg
     8581 gtggcaactc tgccatccgg catttccccg caaatttact cactctctgc cggtaatact
     8641 ggcatccagt caatcggcgt ctcgccacgt tgttccagcc actgatttgc cagcacaaaa
     8701 tggttgcagc caaagaatcc acgatgcgcc gaaagcggcg acggatgcgg tgctttcagt
     8761 acatgatggc gttgcttatc tataatcgcc cctttctttt gcgcatgtga tccccacaac
     8821 aaaaacacca cgccttcgcg gtgctggtta atcaggctga tgactttatc agtaaacgtc
     8881 tcccaaccga ggctggcgtg ggaatgcgcc tgacctgcgc gaaccgtcaa cacagtattg
     8941 agcagcagaa cgccctgacg cgcccagctt tcgagatagc catgattagg gcgggtgaag
     9001 cccggaatag tattttccag ctctttatac atattcaata atgacggagg aatggcaatg
     9061 ccgggacgaa cggaaaatgc cagaccatgc gcctgtcccg gtccgtgata aggatcctgg
     9121 ccgagaatca ccactttaac gtcacccaac tcggtaaagc ggaacgcgtt aaagacatct
     9181 ttttgtggtg gatagatagt gacgccggac tgccgctcgc tggcgacggt ctgaagggta
     9241 ttaagaaaat aaggttgttg cttctcttca gccagcacgt catgccaggt taattcgtta
     9301 gccatctcgc tctcctgcga atcttcaatc cgcctagctt aactgcttct ttctacagaa
     9361 caaaatcatg ctcatttttt agacttaaac gagtgcaaaa aaatgtaaat tggtaaaatt
     9421 tcacacccgc ataagttgat gtaaaacaat aaaatccatt tacgaccaca atcgatgtgt
     9481 ggtttttatt gatttaaatc aaagaatcaa gggtggttga ggagtatata tacactcaag
     9541 caacaatggt tttaccaatt ggccgcgaca ggctgaacaa atcaaataat tttgccgggg
     9601 aggcatcaca tgattacagg tatccagatt actaaagccg ctaacgacga tctgctgaac
     9661 tctttctggc tgctggacag cgaaaaaggc gaagcacgtt gcatcgttgc aaaagcaggt
     9721 tatgcagaag atgaagtggt tgcagtaagc aaactgggtg acattgaata ccgtgaagtt
     9781 ccagtagaag tgaaaccaga agttcgcgtt gaaggtggtc aacacctgaa cgttaacgtt
     9841 ctgcgtcgtg aaactctgga agatgcagtt aagcatccgg aaaaatatcc gcagctgacc
     9901 atccgtgtat ccggttatgc agttcgcttt aactctctga ctccggaaca gcagcgcgac
     9961 gttatcgctc gtacctttac tgaaagcctg taaggcttaa gtagcaagtt actaagcgga
    10021 agagggcgac ctcttccgct ttttttcgtt aatagaaaat gcgtaccgcg caatagacca
    10081 gtaacagggc aagcacgata tttaactggc gaccatactg gcgaaacaat cgctgaaaca
    10141 gatgccccgc cagcgcccag cacacattgc caaacgtccc aatcatcgcc agcaaaacgc
    10201 tgacgccaac tacccagctt agcgcctgtg tttgcggcag aacaaacgtc gacagtgccg
    10261 taacaccgta caaaatgatt ttgacgttca caaactgcaa agcaaagctg gcccaaaagc
    10321 tgattggttt tgcctgaagt ccgtcttcct ttgttgggct ggtggcgatt ttccacgcca
    10381 gccagacaat atatgccgcc cccgcccaac tcaaaaggtg taccgctgcc gggtcaatca
    10441 ctgccagtga aaatgaaatg cccgcacaca gtaacatcac aatcaaaaat cccagactca
    10501 tccctgccag cacgcgggta ctttgacgaa atccatgcga cgtagcagag ctaagggcga
    10561 gaatattgtt cggtcctggc gtcatagcgg taatcagggt gtaagtccaa aaagcactta
    10621 aaagggtcgg tgtcacttgt tatctcctct gttttggtag acagtacccg acacggaaca
    10681 tttaaaaaaa tgaatgtttt tatattctag attcgatatt ttcgataggt ttggggttat
    10741 ggatctgcgc cgttttatta cgcttaaaac cgtggtggaa gagggttcct ttttgcgagc
    10801 ttcgcaaaaa ttgtgctgta cacaatcgac ggtgactttt catattcagc agcttgagca
    10861 ggagttttca gtccagttat ttgagaaaat tggccgacgg atgtgcctta cccgcgaagg
    10921 aaaaaagttg ctgccgcaca tttatgaact tacccgagtg atggatacgc ttcgtgaggc
    10981 tgccaaaaag gagtcagatc cggacgggga actacgcgtt gtttcgggcg aaacgttgct
    11041 ctcttaccga atgccgcagg tgttgcagcg ttttcggcaa cgcgcaccaa aagtgcgttt
    11101 atcgttgcag tcgctgaact gttacgtgat ccgtgatgcg ctactgaatg atgaggccga
    11161 tgtcggcgtc ttttatcgcg tagggaatga tgatgccctg aaccgacgag agttgggtga
    11221 acaatcgctg gtgctggtgg cttcaccaca aattgtagat gtcgatttta ctgagccggg
    11281 aagacataac gcctgtagct ttattatcaa tgaaccacaa tgtgtcttcc ggcagatatt
    11341 tgagagcacg ctgcgtcagc ggcggatcac ggtggaaaac accattgagc ttataagtat
    11401 cgaaagcatc aagcgttgtg tcgcggcgaa tatcggagtc agctacctgc cgcgttttgc
    11461 ggtggcaaaa gagttggagt gcggagaatt aattgaattg ccttttggcg aacagtcgca
    11521 gaccattacg gccatgtgcg cacaccatgc tggaaaagcg gttagcccgg cgatgcacac
    11581 ttttattcag tgtgttgaag agagttttgt ggctggataa aaataatgtc gggattaaac
    11641 ccggcattta acttgtcgac aaagtgcgct ttattcatgc cggatgcggc gtgaacgcct
    11701 tatccggcct acaagatcgt gcaaattcaa tatattgcat gagccacgta ggcctgataa
    11761 gcgtagcgca tcaggcagtt ttgcgtttgc cagcactttt aataccaggc ttaaacccgg
    11821 cattgagatt actcttctgt cgtttgcggt ggcacgcccg ttccgcttgg tttacggcgc
    11881 ttaccaatat ttttggtgtc gcgatggcgt tttttcaccc gcggtttctc tttctcttta
    11941 gcttttttct tctcggcgcg ttttgccagt actttcttcg atggcttgcc ggtctgcttc
    12001 tcgcttggcg cacgcgtttt cgggcgtaac tcatcaataa cgcgagcttt aatcggctct
    12061 tcaatgtaac ggccaacctt gcccagcagc aaatggtcat gggcttccac cagcgaaatt
    12121 gcggtgcctt tacgaccggc gcgcgcggta cgtccgatac ggtgcaaata agtatcgcca
    12181 ctgcgcggca tatcgaagtt aaagacgtgg ctgacgtcag gaatgtcgat accgcgcgcg
    12241 gcaacatcgg ttgcaaccag tacgtttacg cgaccttcgg tcaaacgctt gatcgcttcg
    12301 ttacgcttgc cttgtaccat ctcaccttcg agatagcagt tgttgatgcc cgcttcgcgc
    12361 agccagtttg ccagctcatg cacacgttca cgcttacgca caaacacaat tgagcgggtc
    12421 gcttccggct gttttaacag atgcaccagc aatgcggttt tatgttccag gtcatcggcg
    12481 cggtaatacc actgatgaat ttttttgcgc tcacgggtgg acggattggc agaaacctcc
    12541 accggatctt ccagcagacg ctcggcaaag tcctgaatcg catcgccttc cagcgttgcc
    12601 gaaaagagca gggtctgttt acgccagcgc gtttcgccag caatatgttc gatatcctga
    12661 gcgaagccca tatccagcat acggtctgct tcgtcgagga tcagcgtttc aaccgcgcgg
    12721 caatcgaagt tctcttcttt tatgtattgc agcagacgtc cggtcgtggc gaccacgatg
    12781 tcctgatttt cgctgaacac ttccgcgtgg ttcatatagg ctacgccgcc ggtgatggtg
    12841 gcgatatcca gatgcgtatg tttcgccagt tcgcgggcat gatcggccac ctgcatcgcc
    12901 agctcgcgag ttggggtgag gatcaaaata cgcggcggac cggatttctt acgtgggaaa
    12961 tcgagcaggt gctgcaacgc tggcagcaga tacgccgccg ttttaccggt gcctgtcggc
    13021 gcagaaccga gtacatcacg gccatcgagc gcaggcggaa tggcggcagc ctgaatggcg
    13081 gtcgggcgag tgaaaccttt atcctggagg gcttccagca ggctttcgtc gagttcaagt
    13141 tcggaaaaag tcgttacagt catgttctac ctctgtgtgg ggcgctgatt atagacgtta
    13201 cggctgcaat cttcatctgt ttgtatggat atcgcttctc gggtattgtt ctcacccggt
    13261 gctatcgccg tttttcctgc aaggttttat tttcatgtca cagtctacat ccgtgcttcg
    13321 tcgtaatgga tttactttta aacagttttt tgttgctcac gatcgctgtg cgatgaaagt
    13381 gggaacggat ggcattttgc tgggcgcatg ggcaccggtg gctggggtga aacgttgcct
    13441 tgatatcggc gcgggtagcg ggttgctggc actgatgctg gcgcagcgaa ccagtgacag
    13501 cgtgataatt gatgccgttg agctggaaag tgaagctgcc gcgcaggcgc aggaaaatat
    13561 caaccagtcc ccgtgggcag agcggattaa cgtccatacg gcggatattc agcagtggct
    13621 cacacagcag acagtacgct tcgatttaat catcagtaac ccaccttact atcagcaggg
    13681 agtagagtgc gcgacacctc aacgggaaca ggctcgctat accactacgc ttgatcaccc
    13741 atcgctgctg acctgtgcgg cggagtgcat taccgaagag ggatttttct gcgtggtgct
    13801 gccagagcag gttggtaatg gttttactga actggcatta agcatgggct ggcatttacg
    13861 tttgcggacg gatgtggcgg aaaacgaagc gcgactgccg catcgggtgc tgctggcatt
    13921 ctccccgcag gcgggagaat gctttagcga tcgcttagtc attcgtgggc cagaccagaa
    13981 ctattccgaa gcgtatacgg cgctgaccca ggctttttat ctgtttatgt aatgattgcc
    14041 ggggggaagg atcgatggac cggaatgcgt gagcagttcc gggtaatcca gcgtaaaatg
    14101 caggccacga ctctccttgc gcatcatagc acagcgaaca atcaactcgg caacctgtac
    14161 cagattacgc agctccagca aattattcga cacgcggaag tgggcgtaat attcgtctat
    14221 ttcctgttgc aacatattga tacgccgcag ggcgcgctca agacgttttg ttgtgcgcac
    14281 aatgccgacg taatcccaca taaacagccg tagctcgtgc cagttatgct gaattactac
    14341 ccgttcgtca gggttctcaa cgcggctttc atcccacggt ggtaacgtac tgacgccgtg
    14401 ggcatcaggc atacgtctgg tgatatcttc tgccgccgac cagccataga ccagacactc
    14461 cagcaatgaa ttcgaggcca tgcggttagc gccgtgtaag ccggtataac tcacctcgcc
    14521 aatggcatac aagccctcga cgtccgtacg cccatgatca tcaaccatta caccaccgca
    14581 ggtatagtgt gcagcaggca caatcggtac cggttcttgt gtgagatcaa tccccagccc
    14641 gagcaatttt tcataaatca tcgggaaatg ctggcgaata aaatcggcgg gcttatggct
    14701 gatgtcgagg aacatacaat ctgcgccgag gcgtttcatt tcatggtcaa tggcgcgggc
    14761 gacaatatcg cgcggggcca gttcgccgcg ctcatcaaaa tcgggcataa aacgcgtgcc
    14821 atccgggcgc ttgagataag cgccttcgcc gcgcagtgct tccgttaaca ggaaattgcg
    14881 tgcctgtggg tgatacagtg cggtaggggg gaactgatta aattcgagat tggcaacccg
    14941 actgaggtgt actggcaata gcgga
//
LOCUS       DAJTZL010000100        14841 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.100, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000100 DAJTZL010000000
VERSION     DAJTZL010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14841
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.100"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..2688
                     /locus_tag="OO544_002642"
     CDS             <1..2688
                     /locus_tag="OO544_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HCR6768206.1"
                     /translation="FTAAHGNNTASKITNILDGTVTATSSDAINGSQLYDLSSNIATY
                     FGGNASVNTDGVFTGPTYKIGETNYYNVGDALAAINSSFSTSLGDALLWDATAGKFSA
                     KHGTNGDASVITDVADGEISDSSSDAVNGSQLHGVSSYVVDALGGGAEVNADGTITAP
                     TYTIANADYDNVGDALNAIDTTLDDALLWDADAGENGAFSAAHGKDKTASVITNVANG
                     AISAASSDAINGSQLYTTNKYIADALGGDAEVNADGTITAPTYTIANAEYNNVGDALD
                     ALDDNALLWDETANGGAGAYNASHDGKASIITNVANGSISEDSTDAVNGSQLNATNMM
                     IEQNTQIINQLAGNTDATYIEENGAGINYVRTNDNDLAFNDASASGVGATAVGYNAVA
                     SGASSVAIGQNSSSTVDTGIALGSSSVSSRVIAKGSRDTSVTENGVVIGYDTTDGELL
                     GALSIGDDGKYRQIINVADGSEAHDAVTVRQLQNAIGAVATTPTKYFHANSTAEDSLA
                     VGEDSLAMGAKTVVNGNAGIGIGLNTLVLADAINGIAIGSNARANHANSIAMGNGSQT
                     TRGAQTGYTAYNMDAPQNSVGEFSVGSEDGQRQITNVAAGSADTDAVNVGQLKVTDER
                     VAQNTQSITNLNNQVTNLDTRVTNIENGIGDIVTTGSTKYFKTNTDGVDANAQGKDSV
                     AIGSGSIAAADNSVALGTGSVAEEENTISVGSSTNQRRITNVAAGVNATDAVNVSQLK
                     SSEAGGVRYDTKADGSIDYSNITLGGGNGSTTRISNVSAGVNNNDAVNYAQLKQSVQE
                     TKQYTDQRMVEMDNKLSKTESKLSGGIASAMAMTGLPQAYTPGASMASIGGGTYNGES
                     AVALGVSMVSANGRWVYKLQGSTNSQGEYSAALGAGIQW"
     gene            3056..4711
                     /gene="lldP"
                     /locus_tag="OO544_002643"
     CDS             3056..4711
                     /gene="lldP"
                     /locus_tag="OO544_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HCR6768207.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASKIDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            4711..5487
                     /gene="lldR"
                     /locus_tag="OO544_002644"
     CDS             4711..5487
                     /gene="lldR"
                     /locus_tag="OO544_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HCR6768208.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            5484..6674
                     /gene="lldD"
                     /locus_tag="OO544_002645"
     CDS             5484..6674
                     /gene="lldD"
                     /locus_tag="OO544_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HCR6768209.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            6870..7343
                     /gene="trmL"
                     /locus_tag="OO544_002646"
     CDS             6870..7343
                     /gene="trmL"
                     /locus_tag="OO544_002646"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HCR6768210.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(7396..8217)
                     /gene="cysE"
                     /locus_tag="OO544_002647"
     CDS             complement(7396..8217)
                     /gene="cysE"
                     /locus_tag="OO544_002647"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HCR6768211.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(8297..9316)
                     /gene="gpsA"
                     /locus_tag="OO544_002648"
     CDS             complement(8297..9316)
                     /gene="gpsA"
                     /locus_tag="OO544_002648"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HCR6768212.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(9316..9783)
                     /gene="secB"
                     /locus_tag="OO544_002649"
     CDS             complement(9316..9783)
                     /gene="secB"
                     /locus_tag="OO544_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HCR6768213.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSVVSRGTFPQLNLAPINFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(9846..10097)
                     /gene="grxC"
                     /locus_tag="OO544_002650"
     CDS             complement(9846..10097)
                     /gene="grxC"
                     /locus_tag="OO544_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HCR6768214.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(10239..10670)
                     /locus_tag="OO544_002651"
     CDS             complement(10239..10670)
                     /locus_tag="OO544_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCR6768215.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            10915..12459
                     /gene="gpmM"
                     /locus_tag="OO544_002652"
                     /gene_synonym="pgmI"
     CDS             10915..12459
                     /gene="gpmM"
                     /locus_tag="OO544_002652"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HCR6768216.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            12469..13752
                     /gene="envC"
                     /locus_tag="OO544_002653"
     CDS             12469..13752
                     /gene="envC"
                     /locus_tag="OO544_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HCR6768217.1"
                     /translation="MRGKAINTMTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADE
                     RDQLKSIQADIATKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNK
                     QIDEMNASIAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYF
                     GYLNQARQETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKK
                     TLAGLESSIQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEA
                     TRKGTTYKPTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIG
                     ASEGTEVKAIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQ
                     PIALVGSSGGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            13756..14715
                     /locus_tag="OO544_002654"
     CDS             13756..14715
                     /locus_tag="OO544_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HCR6768218.1"
                     /translation="MFPFRRNVLAFAALLALSSPVIAGKLAIVIDDFGYRPNNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNTVPDTPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKLNASVN"
BASE COUNT         3539 a         3954 c         3961 g         3387 t
ORIGIN      
        1 ttcactgcag ctcatggcaa caataccgcc agcaaaatca ccaatatcct ggacggcaca
       61 gtcactgcaa ccagttccga tgccattaac ggtagccagc tttatgactt aagcagcaat
      121 atcgccacct acttcggcgg caatgcttct gtgaatactg acggtgtgtt taccggtcca
      181 acctacaaaa tcggtgaaac aaattattat aacgtcggcg atgcactggc tgcgattaac
      241 tcctcattta gcacgtctct cggcgatgct ctgctttggg atgccaccgc aggtaaattc
      301 agtgccaaac acggtactaa tggtgacgca agcgtgatca ctgatgtcgc agatggtgaa
      361 atttcagact ccagttctga cgcagtaaac ggctcacaac tccacggcgt gagcagttat
      421 gttgttgatg cgctgggggg tggtgccgaa gtcaatgcag acggcaccat cactgcgccg
      481 acgtacacca ttgctaatgc tgattacgat aatgtcggtg atgccctgaa tgctatcgat
      541 accactcttg acgacgctct gctctgggat gcggacgccg gtgaaaatgg tgcatttagc
      601 gccgctcacg gaaaagataa aactgccagt gtaatcacta acgtcgctaa cggtgcaatc
      661 tctgctgcca gcagcgacgc gattaacggc tcacaactct ataccaccaa taagtacatc
      721 gctgatgcgc tgggtggtga cgcagaagtc aacgctgacg gcaccatcac cgcaccgact
      781 tacaccattg cgaacgccga gtacaacaac gtcggtgacg ccctggatgc gcttgatgat
      841 aacgccctgc tgtgggatga gactgccaat ggcggtgctg gagcctacaa tgccagccat
      901 gacggtaaag ccagcatcat cactaatgtc gctaatggca gtattagtga ggacagtacc
      961 gatgcagtga acggttctca gttgaatgcg acgaatatga tgattgagca gaacacccaa
     1021 attatcaatc agctcgctgg taacaccgac gcaacctata tcgaagaaaa tggcgctggt
     1081 atcaactacg ttcgtactaa cgacaacgat ttagccttta acgatgcaag cgcctctggt
     1141 gtcggcgcta cagctgtagg ttataacgct gtcgcgtctg gtgccagcag cgtagccatt
     1201 ggtcagaaca gcagcagcac cgttgatacc ggtattgcgc tgggtagcag ctccgtttcc
     1261 agccgtgtga ttgccaaagg ttctcgtgac actagcgtaa cggaaaatgg cgtggtgatt
     1321 ggttatgaca ccactgacgg cgaactgcta ggtgcattgt caattggtga tgacggtaaa
     1381 taccgccaaa tcatcaacgt agctgatggt tcagaagccc atgacgccgt tacggttcgc
     1441 cagttgcaga acgctattgg agcggtcgcc actacgccaa ccaagtactt tcacgccaac
     1501 tcaacggcag aagactcact ggccgttggt gaagactcgc tggcaatggg tgcgaaaact
     1561 gtcgttaatg gtaatgcagg tattggtatc ggtttgaaca ctctggttct agctgatgcg
     1621 atcaacggca ttgctatcgg cagtaacgca cgcgcaaacc atgcaaacag tatcgcaatg
     1681 ggtaacggtt ctcagaccac ccgtggtgca cagactggct acaccgccta caacatggac
     1741 gcaccgcaga actctgtagg tgagttttct gtcggtagtg aagacggtca acgtcagatc
     1801 acaaacgtcg cagctggttc agcggatacc gatgcggtta acgtgggtca gttgaaagtc
     1861 actgatgagc gcgtagcgca aaatacccag agcattacta acctgaacaa tcaggtcact
     1921 aatctggata ctcgcgttac taatatcgaa aacggtattg gcgacattgt caccaccggt
     1981 agcaccaagt acttcaagac caacaccgat ggcgtagatg ccaacgccca gggtaaagat
     2041 agcgttgcta ttggttctgg ttccattgct gccgctgaca acagcgtcgc actgggtacc
     2101 ggttccgttg cagaggaaga aaatacaatc tctgtaggtt cttctactaa ccaacgccgt
     2161 atcaccaacg tagctgccgg tgttaatgcc accgatgcgg ttaacgtgtc gcagctgaaa
     2221 tcttctgaag caggcggcgt gcgctacgac accaaagctg atggttctat cgactatagc
     2281 aatatcaccc tcggtggtgg caacggcagt acgactcgta tcagcaacgt ctccgctggc
     2341 gtcaacaaca acgacgcggt gaactacgcg cagttgaagc aaagcgtgca ggaaacgaag
     2401 caatacaccg atcagcggat ggttgagatg gataacaaac tgtctaaaac tgaaagcaag
     2461 ttgagcggtg gtatcgcttc tgcaatggca atgaccggtc tgccgcaggc ttatacaccg
     2521 ggtgccagca tggcttctat tggtggcggt acttacaacg gtgaatcggc agttgcttta
     2581 ggtgtatcga tggtgagcgc caatggtcgt tgggtctaca aattacaagg tagtaccaat
     2641 agccagggtg aatactccgc cgcactcggt gccggtattc agtggtaatg atccattaag
     2701 ttagtgtgac taagggcaaa cgccaaattg cctgatgcgt tacgcgcatc aggcaagata
     2761 aggcgccccg ggtcaacaat ctaagggcaa ttctctgatg aggattgccc ttttctttac
     2821 cagacatctc cccccacaag aattggccct accaattctt cgcttatctg acctctggtt
     2881 cacaattccc caattaaaac tcacatcaat attgccaata cataacattt agttaaccat
     2941 tcattgtcat tatccctaca caacacaatt ggcagtgcca cttttacaca acgtgtgaca
     3001 aggagatgag caacagactc attacacgat gtgcgtggac tccaggagac ctgcaatgaa
     3061 tctctggcaa caaaactacg atcccgccgg gaatatctgg ctttccagtc tgatagcatc
     3121 gcttcccatc ctgtttttct tctttgcgct gattaagctc aaactgaaag gatacgtcgc
     3181 cgcctcatgg acggtagcaa tcgcccttgc cgtggctttg ctgttctata aaatgccggt
     3241 cgctaacgcg ctggcctcgg tggtttatgg cttcttctat ggtttgtggc ccatcgcgtg
     3301 gatcattatt gcagcagtgt tcgtctataa gatctcggtg aaaaccgggc agtttgacat
     3361 cattcgctcg tctattcttt cgataacccc tgaccagcgt ctgcaaatgc tgatcgtcgg
     3421 tttctgtttc ggcgctttcc ttgaaggagc cgcaggcttt ggcgcaccgg tagcaattac
     3481 cgccgcattg ctggtcggcc tgggttttaa accgctgtac gccgccggac tgtgcctgat
     3541 tgttaacacc gcgccagtgg catttggcgc gatgggcatt ccaatcctgg ttgccggaca
     3601 agtaacaggc atcgacagct ttgagattgg ccagatggtg gggcggcagc taccgtttat
     3661 gaccattatc gtgctgttct ggatcatggc gattatggac ggctggcgcg gtatcaagga
     3721 gacgtggcct gcggtcgtgg ttgcgggcgg ctcgtttgcc atcgctcagt accttagctc
     3781 taacttcatt gggccggagc tgccggacat tatctcttcg ctggtatcac tgctctgcct
     3841 gacgctgttc ctcaaacgct ggcagccagt gcgcgtattc cgttttggtg atttgggggc
     3901 gtcaaagatt gatatgacgc tggcccacac cggttacact gcgggtcagg tgttacgtgc
     3961 ctggacaccg ttcctgttcc tgacagctac cgtaacactg tggagcatcc cgccgtttaa
     4021 agccctgttc gcatcgggtg gtgcgctgta tgagtgggtg atcaacattc cggtgccgta
     4081 cctcgataaa ctggttgccc gtatgccacc agtggtcagc gaggctacag cctatgccgc
     4141 cgtgtttaag tttgactggt tctctgccac cggcaccgcc attctgtttg ctgcactgct
     4201 ctcgattgtc tggctgaaga tgaaaccgtc tgacgctatc agcaccttcg gcagcacgct
     4261 gaaagaactg gctctaccca tctactccat cggcatggtg ctggcgttcg cctttatctc
     4321 gaactattcc ggactgtcat caacactggc actggcactg gcgcacaccg gtcatgcatt
     4381 caccttcttc tcgccgttcc tcggctggct gggggtattc ctgaccgggt cggatacctc
     4441 atctaacgcc ctgttcgccg cgctgcaagc caccgcagca caacaaattg gcgtctctga
     4501 tctgttgctg gttgccgcca ataccaccgg tggcgtcacc ggtaagatga tctccccgca
     4561 atctatcgct atcgcctgtg cggcggtagg cctggtgggc aaagagtctg atttgttccg
     4621 ctttactgtc aaacacagcc tgatcttcac ctgtatggtg ggcgtgatca ccacgcttca
     4681 ggcttatgtc ttaacgtgga tgattcctta atgattgttt tacccagacg cctgtcagac
     4741 gaggttgccg atcgtgtgcg ggcgctgatt gatgaaaaaa acctggaagc gggcatgaag
     4801 ttgcccgctg agcgccaact ggcgatgcaa ctcggcgtat cacgtaattc actgcgcgag
     4861 gcgctggcaa aactggtgag cgaaggcgtg ctgctcagtc gacgcggcgg cgggacgttt
     4921 attcgctggc gtcatgacac atggtcggag caaaacatcg tccagccgct aaaaacgctg
     4981 atggccgatg atccggatta cagtttcgat attctggaag cccgctacgc cattgaagcc
     5041 agcaccgcat ggcatgcggc aatgcgcgcc acacctggcg acaaagaaaa gattcagctt
     5101 tgctttgaag caacgctaag tgaagacccg gatatcgcct cacaagcgga cgttcgtttt
     5161 catctggcga ttgccgaagc ctcacataac atcgtgctgc tgcaaaccat gcgcggtttc
     5221 ttcgatgtcc tgcaatcctc agtgaagcat agccgtcagc ggatgtatct ggtgccaccg
     5281 gttttttcac aactgaccga acaacatcag gctgtcattg acgccatttt tgctggtgat
     5341 gccgacgggg cgcgtaaagc aatgatggcg caccttagtt ttgttcacac caccatgaaa
     5401 cgattcgatg aagatcaggc tcgccacgca cggattaccc gcctgcccgg tgagcataat
     5461 gagcattcga gggagaaaaa cgcatgatta tttccgcagc cagcgattat cgcgccgcag
     5521 cgcaacgcat tctgccgccg ttcctgttcc actatatgga tggtggtgca tattctgaat
     5581 acacgctgcg ccgcaacgtg gaagatttgt cagaagtggc actgcgccag cgtattctga
     5641 aaaacatgtc cgacttaagc ctggaaacga cgctgtttaa tgagaaattg tcgatgccgg
     5701 tagcactggc accggtgggt ttgtgtggca tgtatgcgcg tcgtggcgaa gttcaggcag
     5761 ccaaagcggc ggacgcgcat ggcattccgt ttactctctc gacggtttcc gtttgcccga
     5821 ttgaagaagt cgcgccagcc atcaagcgcc caatgtggtt ccagctttat gtactgcgcg
     5881 atcgcggctt tatgcgtaac gcgctggagc gagcaaaagc agcgggttgt tcgacgctgg
     5941 ttttcaccgt ggatatgccg acaccgggcg cacgctaccg tgatgcgcat tcaggtatga
     6001 gcggcccaaa tgcggcaatg cgccgctact tgcaggcggt gacgcatccg caatgggcgt
     6061 gggatgtggg cctgaacggt cgtccacatg atttaggtaa tatctcagct tatctcggca
     6121 aaccgaccgg actggaagat tacatcggct ggctggggaa taacttcgat ccgtccatct
     6181 catggaaaga ccttgagtgg atccgcgatt tctgggatgg cccgatggtg atcaaaggga
     6241 tcctcgatcc ggaagatgcg cgcgatgcag tacgttttgg tgctgatgga attgtggttt
     6301 ctaaccacgg tggccgccag ctggacggtg tactctcttc cgctcgtgcg ttgcccgcta
     6361 ttgcagatgc ggtgaaaggt gatatcgcca ttctggcgga tagcggaatt cgtaacgggc
     6421 ttgatgtcgt gcgtatgatt gcgctcggtg ccgacaccgt actgctgggt cgtgctttcc
     6481 tgtatgcgct ggcaacagcg ggccaggcgg gtgtagctaa cctgctaaat ctgatcgaaa
     6541 aagagatgaa agtggcgatg acgctgactg gcgcgaaatc gatcagtgaa attacgcaag
     6601 attcgctggt gcaggggctg ggtaaagagt tgcctacggc actggctccg atggcgaaag
     6661 ggaatgcggc atagtcgttt gcgccccctc accctaaccc tctccctcag ggagagggga
     6721 ccgttcggcg ctgtatgtac tccctcactc tgaaacgaca ccgcactctt tttttctccc
     6781 tcgcccctcc ggggagggcc ggggtgaggg gaaaaggccg cacggttcac caattttctg
     6841 ctatcctgcc cccgcactaa gggggcagta tgctaaacat cgtactttac gaaccagaaa
     6901 ttccgccaaa tactggcaac atcatccgtc tttgcgctaa taccggcttt cgtctgcata
     6961 tcatcgaacc gatgggattt gcctgggacg ataagcgcct gcgccgcgcg gggctggact
     7021 atcacgagtt taccgccgtt acgcgtcatc atgactatcg cgcgttcctc gaagcagaaa
     7081 atccccagcg cctgtttgcc ctcaccacga aaggtacgcc tgctcacagc gccgtaagct
     7141 atcaggatgg cgattatctg atgttcggcc cggaaacacg cggcctgcca gcgagcattc
     7201 ttgatgccct gcccgctgaa caaaaaattc gcattccgat ggtgccggac agccgcagca
     7261 tgaatctgtc caatgcggtg tcggtagtgg tgtatgaagc ctggcggcag ctgggatatc
     7321 cgggagcgtt gttgagagat tagttactgt atgccggata gatgattaca tcgcatccgg
     7381 cacgatcaca ggacattaga tcccatcccc atactcaaat gtatggttaa taccgttgaa
     7441 atgctggtcc atatccattg atggcttatc gctgtctggt ttaccgacaa tacgagccgg
     7501 aacgccagcg gcggttgtat gcggcggcac cggttgcagc accacggaac ctgcgccaat
     7561 cttcgcgccg cgaccaactt caatattgcc gaggattttc gcgcccgcgc caatcatcac
     7621 accttcacga attttcgggt gacggtcacc accagattta cccgtaccgc caagcgtcac
     7681 agattgcaga atcgatacgt cgttttcaat taccgccgtt tcacccacaa cgatgcccgt
     7741 ggcgtggtcg agcataatgc cacggccaat ttttgctgcc gggtgaatat cgacctggaa
     7801 cgtcacagaa acctggtttt gcagaaagat tgccaatgcg cgacgcccct gattccacaa
     7861 ccagtgaccg atgcgatagg cctgcaaggc atgaaaaccc ttcaggtata acaacggggt
     7921 tgagtattta tcgactgccg ggtcgcgggt acgcaccgcc tgaatatcac aggccgcaga
     7981 ggcgatcatt tccgggtcag cggcgtaggc ttcttccacc acttcacgga tagcaatagc
     8041 aggcataatt ggcgatgaca gcttgttcgc cagcatgtag ctcagtgcac taccaaggtt
     8101 ttcgtgcttg agtagcgtcg cgtggtaaaa actggccagc attggctcac agtccgccag
     8161 cgttctggct tcggctttaa tattgttcca gacaatttcc agttcttcac acgacattgc
     8221 ttactccaca cgatgagata atgaccggcc cgttctgcgc gggccgggtc atagcggtaa
     8281 caaaggttcc ctggggttag tggctgctgc gctcgtcctt gcgtgcacga cctaataacg
     8341 tcaatgctgc ctcgcgcgcg ttttttccgc aatataatac ttgataaatt tcctcggtta
     8401 ttggcatttc tacgccgaag cgatgcgcca gttcgcggac ttctttcgta ttgcggtagc
     8461 cttccaccac ctgaccaatc ttctcctgcg cgctttgtac atccatgccc tgaccgagca
     8521 tcatgccaaa acggcggtta cgcgactggt tgtcggtaca ggtaagcacc agatcgccca
     8581 gccccgccat gcccataaag gtggcaggat cggcacccag cgccgcgcca agacgcgaca
     8641 tttcagccag cccacgggtg atcagtgccg tacgcgcatt cgcaccaaaa ccgataccgt
     8701 cggacatccc cgcgccaatg gcaatgacgt ttttcaccgc accgccaagc tgcacaccga
     8761 tgaaatccgg gttgctgtaa acgcggaaac ttttgccaca gtgcagcaat tgttggagat
     8821 catcggcaaa ggtctggtcg gtagaggcca acgaaatagc cgtcggtaaa cctgccgcca
     8881 gttctttcgc aaacgttggg ccagagataa ccgccagcgg aatttgatcg cctaaggcct
     8941 cacgcgccac gtcctgtaac agacgtccgg tttccgcttc cagccctttg gtcgcccaca
     9001 ccagacgcgc atcaggacgc atcagcggtt taatctggcg cagcacttca ccaaagacat
     9061 ggctgggtac gacgacgaga atattacggc tggctgccag cgcagtggcg agatcgcttt
     9121 caagatggag cgtatcggga aaaggcacat cggggagaaa cgcggcgtta cagcggtcgc
     9181 gttcaagcgt tgcgatatgt tcagggtcat ggccccagag gacaacctcg tggccatttc
     9241 ttgccagggt aatggcaaga gcggtgccgt acgagccggc accgatcaca gtcattgaag
     9301 cattacgttg gttcatcagg catcctgatg ttcttcagta ccttcgccag cctgctgctg
     9361 caaatagttc atgaacagcg catcgaagtt aatcggcgca aggttcagtt gcgggaatgt
     9421 accgcgggat accacgctgg tgatgcactc acgagcatac gggaacagaa tgttcgggca
     9481 gtatgcgccc aggcaatgtg ccatctgggt gccttcgata cccgcgatgg agaaaatacc
     9541 gccctgctga acttcacaca ggaacgcggt ttcttcgccc agggaggccg ttacggtaac
     9601 acgcagtacc acttcgtata cgtcatctgc cagttgggaa gatgccgtat ccagatcaag
     9661 tttaacttct ggttgccaat ctttctggaa aacgtgcggc gcgttcggcg cttcgaaaga
     9721 gatatccttg gtataaatac gttggatctg gaaagtcatt tcagtgttgt tttgttctga
     9781 catgtgtaga aaacccttaa gtgttgtcct taaatactgc gcaatgtaat gccaacgttc
     9841 acacgttatt tcagcagggg atccagtcca ccacgtgcat ccaatgcata caagtcatca
     9901 cagccgccaa tgtgctgtgc gtcaataaaa atctggggaa ccgtggtgcg accgctgcgt
     9961 ttgatcatct cttcacgctt ggcggcattg ccatcgatcg gcaactcctg aaaactcacg
    10021 cccttgctgc tcagcagtgc ttttgcacga tggcaatacg ggcaggtttc tttggtatag
    10081 atttcaacat tggccataat ttagctcctg tttttttacc ctgaaatttc atgttgcagg
    10141 gcggctgcaa aatggagtgt ccccagaagc ttataacaat aagtcgctgg gggcctaatt
    10201 gcagctaacg gcctgcatca tgaaagacga caggtaaatt atttgccgcg caccagaggc
    10261 aagttttcgc cagcccagcc agcaacgccc tctttcagca cgaatacttg cgcaaaacca
    10321 gctttcgtca gcgcgtttgc aggctcctgg cactgcatgc cagaaccgtc taccacgata
    10381 accggtttgt ctttgtgctt ctcaagctca ccaacattgt tggctttgat ttcgctcggc
    10441 aacagattga tagaacctgc gatatggcct ttacggaagt catcacgctg acgtaaatcc
    10501 acaaccacag cgtcttcttt gttgatcaga cgcgtagctt caccacgagt aatcaccttc
    10561 actttcgagg tcaggctctt aaacgtagtc acaagaaccg ccaccagtaa cgcgatccag
    10621 gcgatactca gtatgggatg acggccaaca aattgcataa tttcttgcat ggggggtaac
    10681 aactcccgac gtagtgatta aaaaaaccag gaaaggagta tacctgcgcg gtgcggcaaa
    10741 tacagccagc gcgttaactg gaatgcaatt ttgcggggca cgacgaaaaa aaagcgcaaa
    10801 tctggcatcg gcttgccgcg agcggtcgta tattttgatc ttcagaggct attttatcga
    10861 ttcagctgta gtaaaattac gcaaattttg actcttgagt atgaggttgt cgcaatgtcg
    10921 gtttctaaaa aacctatggt actggtgatt ctggatggct atggctatcg tgaagaacag
    10981 caggataacg ccatttttag tgctaaaacc ccggtaatgg atgcactgtg ggccaatcgt
    11041 ccgcataccc tgatcgacgc ttccggtctg gaagtcggtc tgcctgaccg tcagatgggt
    11101 aactccgaag taggccacgt taacctgggt gccggccgca tcgtgtatca ggacctgact
    11161 cgtctggacg ttgaaatcaa agatcgcgct ttctttgcta atccagtgct gactggcgcg
    11221 gtagataaag cgaaaaacgc aggtaaagca gtacacatta tgggtctgct ctccgcaggc
    11281 ggcgtacaca gccacgaaga tcacatcatg gcgatggtag aactggcagc tgaacgcggc
    11341 gcagaaaaaa tctacctgca cgcattcctt gacggtcgcg acaccccgcc gcgcagtgct
    11401 gaatcctcgc tgaaaaaatt tgaagagaaa tttgctgcgc tgggtaaagg ccgcgtagcg
    11461 tccatcattg gtcgttacta cgcgatggac cgcgataacc gttgggatcg cgttgaaaaa
    11521 gcctatgacc tgctgactct ggcgcagggt gagtttcagg ccgatactgc cgttgctggt
    11581 ttgcaggcag cttatgctcg cgacgaaaac gacgagttcg tgaaagcgac cgttattcgt
    11641 gcagaaggtc agccagatgc ggcaatggaa gacggcgatg cgctgatttt catgaacttc
    11701 cgtgctgacc gcgcgcgtga aatcactcgt gcattcgtga acgctgattt cgatggcttc
    11761 gcgcgtaaga aagtggttaa cgtcgatttc gtgatgctga ccgaatacgc cgctgacatc
    11821 aaaactgcgg ttgcttaccc acccgcttcc ctggttaaca ccttcggcga gtggatggcg
    11881 aaaaacgaca aaactcagtt gcgtatttcc gaaaccgaaa aatatgccca cgttactttc
    11941 ttcttcaacg gtggcgtaga agagtcgttc aaaggcgaag atcgcattct gatcaactca
    12001 ccgaaagtgg ctacctacga tctgcaaccg gaaatgagct ccgcagagct gaccgaaaaa
    12061 ctggttgcgg ccatcaagag cggcaaatac gacaccatta tctgtaacta tccgaacggc
    12121 gacatggtag gtcacaccgg ggtgatggaa gcggcggtta aagcggttga agcactggat
    12181 cactgcgtgg aagaagtcgc gaaagcggtt gagtccgtgg gtggacaact gctgatcacc
    12241 gctgaccacg gtaacgctga acagatgcgc gatccggcaa cgggtcaggc acacacggca
    12301 cacaccaacc tgccagttcc gctgatttac gttggtgata agaacgtgaa agcggttgca
    12361 ggcggcaaac tttctgacat cgcgccgacc atgttgtcgc tgatgggtat ggaaatcccg
    12421 caagagatga ctggtaagcc gctgttcatc gtggaataat ccctccccat gaggggaaag
    12481 gcgattaata ccatgacacg ggccgtgaaa ccgcgcaggt ttgcaatcag gcccatcatc
    12541 tacgccagcg ttcttagcgc tggcgtattg ttgtgcgcct tttccgccca cgcggatgag
    12601 cgtgaccaac tcaaatctat tcaggccgat atcgccacaa aagagcgcgc ggtacgccaa
    12661 aagcaacaac aacgcgcaag cctgctcgca caattgaaaa agcaggaaga agcgatctct
    12721 gaagccaccc gtaagctgcg cgaaacgcaa aacacgctca atcaactcaa taaacagatt
    12781 gatgagatga acgcgtcgat tgccaaactg gagcagcaaa aagccgccca ggagcgcagc
    12841 ctcgccgcac aactggatgc cgcattccgt cagggcgagc ataccggtat tcagctaatt
    12901 ctcagcggtg aagaaagcca gcgtggacag cgtttacagg cttatttcgg ctatctcaac
    12961 caggcgcgac aagaaaccat tgcccagttg aagcaaacgc gtgaagaagt cgccatgcag
    13021 cgtgctgaac tggaagagaa acaaagcgag caacaaacgc tgttatatga acagcgcgcc
    13081 caacaggcga aactgactca ggctctgaac gagcgtaaaa agacgctggc ggggctggaa
    13141 tcttccatcc agcaaggtca gcagcagcta agtgaactgc gtgccaacga atcccgtctg
    13201 cgtaacagta ttgcccgtgc ggaagccgcg gcgaaagcgc gtgctgaacg agaagcgcgt
    13261 gaggctcagg cagttcgcga caggcagaaa gaagcgacgc gcaaaggcac cacctacaaa
    13321 ccgaccgaaa gcgaaaaatc gctgatgtcc cgaactggtg gcctgggtgc gccgcgtggt
    13381 caggcattct ggccagttcg tggccctacg ctgcatcgct atggcgagca gctacagggt
    13441 gaattacgct ggaaaggaat ggttatcggt gcctctgaag gtactgaagt taaagcaatt
    13501 gccgacggtc gggtgattct ggctgactgg ctgcaaggct acggtctggt ggtggtggtt
    13561 gagcacggta aaggcgatat gagtctttac ggctataatc aaagcgcgct ggtgagcgtt
    13621 ggttcgcagg ttcgtgcggg ccagccaatt gcactggtgg gcagcagtgg cggtcagggc
    13681 agaccttcac tctatttcga aattcgccgc cagggtcagg ccgtcaatcc acagccgtgg
    13741 ttgggaagat aagttttgtt tccatttcgt cgtaacgttc ttgcatttgc cgctctgttg
    13801 gcgctctcct cccctgtaat tgctggcaaa cttgccatcg tcattgatga ttttgggtat
    13861 cgcccgaaca acgaaaacca ggtgctggcg atgccttccg ctatctccgt cgctgtatta
    13921 cccgattcac cgcacgccag agagatggcg accaaagcgc ataacagcgg tcacgaagtg
    13981 ttgattcatc tcccgatggc accgttgagt aaacagccgc tggagaaaaa tacgctgcgc
    14041 ccggagatga gcagcgacga aattgagcgc attattcgta gtgcggtcaa taacgtgccc
    14101 tatgccgtgg ggatcaataa ccacatgggc agcaagatga cctctaacct gtttggtatg
    14161 cagaaagtga tgcaggcgct ggagcgttat aatctttact tcctcgacag cgtaaccatt
    14221 ggtaataccc aggcgatgcg cgccgcgcaa ggcactggcg tgaaggtgat caaacggaag
    14281 gtgttcctcg acgattcaca gaacgaagcg gacatccgtg tgcaattcaa tcgcgcaatt
    14341 gacctagcgc gtcgcaacgg atcaaccatt gccattgggc atcctcaccc ttcaacggta
    14401 cgcgtgttgc aacagatggt ttataacctg ccgccagaca ttacgctggt gaaagccagc
    14461 agcttgctga atgaaccgca ggttgatacg tctacaccgc cgaaaaacac tgtgcccgac
    14521 acaccgcgta atccgttccg tggcgtgaag ctgtgcaaac cgaagaaacc gatagaacct
    14581 gtttatgcta atcgcttctt tgaagtatta agcgaaagca tcagccagag cacgctgatc
    14641 gtttacttcc agcatcagtg gcaaggctgg ggcaaacagc ctgaagcggc gaagcttaac
    14701 gctagcgtaa attaaaggcg ctacgcgtaa tgcgatggtg cgaccgcgtt ttgtcgcgcc
    14761 attgccataa gcggaaagac tacagtaacg cctgataacc cacctttacg ctgcgcacat
    14821 tggtaatcag gcgcttatac a
//
LOCUS       DAJTZL010000101        14404 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.101, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000101 DAJTZL010000000
VERSION     DAJTZL010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14404
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.101"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            162..1697
                     /gene="gadC"
                     /locus_tag="OO544_002655"
     CDS             162..1697
                     /gene="gadC"
                     /locus_tag="OO544_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HCR6768219.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            1828..3147
                     /locus_tag="OO544_002656"
     CDS             1828..3147
                     /locus_tag="OO544_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 10 protein"
                     /protein_id="HCR6768220.1"
                     /translation="MDICSRNEKLAIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            3519..4901
                     /gene="dosC"
                     /locus_tag="OO544_002657"
     CDS             3519..4901
                     /gene="dosC"
                     /locus_tag="OO544_002657"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HCR6768221.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIAVAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKILLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRNLNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSQAFYDNVHSSDYVFRYGGDEFIIVLTEASENETLR
                     TAERIRSRVEKTKLKAANGEDIALSLSIGAAMFNGHPDYERLIQIADEALYIAKRRGR
                     NRVELWKASL"
     gene            4926..7325
                     /gene="dosP"
                     /locus_tag="OO544_002658"
                     /gene_synonym="pdeO"
     CDS             4926..7325
                     /gene="dosP"
                     /locus_tag="OO544_002658"
                     /gene_synonym="pdeO"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase"
                     /protein_id="HCR6768222.1"
                     /translation="MKLTDAENAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKL
                     WGYKREEVIGNNIDMLIPRDLRPAHPEYIRHNREGGKARVEGMSRELQLEKKDGSKIW
                     TRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQ
                     CNRAFTEMFGYCISEASGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRT
                     GEKIWIKASISPVYDVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGE
                     IICRNIESVLNESHVSLFALRNGMPIHWASSSHGAEVQNAQSWSATIRQRDGAPAGIL
                     QIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHN
                     YLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFREKLKPDQYLCRI
                     EGTQFVLVSLENDVSNITQIADELRNVVSKPIMIDDKPFPLTLSIGISYDVGKNRDYL
                     LSTAHNAMDYIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAISNNQLKLVYQPQIF
                     AETGELYGIEALARWHDPQHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRS
                     QNIHIPALSVNLSALHFRSNQLPNQVSDAMQAWGIDGHQLTVEITESMMMEHDTEIFK
                     RIQILRDMGVGLSVDDFGTGFSGLSRLVSLPVTEIKIDKSFVDRCLTEKRILALLEAI
                     TSIGQSLNLTVVAEGVETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWMSSVLPLK
                     I"
     gene            7584..8165
                     /gene="ddpX"
                     /locus_tag="OO544_002659"
     CDS             7584..8165
                     /gene="ddpX"
                     /locus_tag="OO544_002659"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HCR6768223.1"
                     /translation="MSDTTELVDLAVIFPDLEIELKYACADNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGLQLVIYDAYRPQQAQAMLWQACPDPQYVVDVTVGSNHSRGT
                     AIDLTLRDEHGNILDMGAGFDEMHERSHAYHPSVPPAAQRNRLLLNAIMTGGGFVGIS
                     SEWWHFELPQAASYPLLADQFSCFISPGTQHVS"
     gene            8179..9729
                     /locus_tag="OO544_002660"
     CDS             8179..9729
                     /locus_tag="OO544_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR6768224.1"
                     /translation="MKRSISFRPTLLALVLATTFPVAHAAVPKDMLVIGKAADPQTLD
                     PAVTIDNNDWTVTYPSYQRLVQYKTDGDKGSTDVEGDLASSWKASDDQKEWTFTLKDN
                     AKFADGTPVTAEAVKLSFERLLKIGQGPAEAFPKDLKIDTPDEHTVKFTLSQPFAPFL
                     YTLANDGASIINPAVLKEHAADDARGFLAQNTAGSGPFMLKSGQKGQQLVLVPNPHYP
                     GNKPNFKRVSVKIIGESASRRLQLSRGDIDIADALPVDQLNALKQENKVNVAEYPSLR
                     VTYLYLNNSKAPLNQADLRRAISWSTDYQGMVNGILSGNGKQMRGPIPEGMWGYDATA
                     MQYNHDETKAKAEWDKVTSKPTSLTFLYSDNDPNWEPIALATQSSLNKLGINVKLEKL
                     ANATMRDRVGKGDYDIAIGNWSPDFADPYMFMNYWFESDKKGLPGNRSFYENSEVDKL
                     LRNALATTDQTQRTRDYQQAQKIVIDDAAYVYLFQKNYQLAMNKEVKGFVFNPMLEQV
                     FNINTMSK"
     gene            9731..10753
                     /locus_tag="OO544_002661"
     CDS             9731..10753
                     /locus_tag="OO544_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6768225.1"
                     /translation="MTFWSILRQRCWGLVLVVAGVCVITFIISHLIPGDPARLLAGDR
                     ASDAIVENIRQQLGLDQPLYVQFYRYFSDLFHGDLGTSIRTGRPVLEELRIFFPATLE
                     LAFCALLLALLIGIPLGILSAVWRNRWLDHLVRIMAITGISTPAFWLGLGVIVLFYGH
                     LQILPGGGRLDDWLDPPTHVTGFYLLDALLEGNGEVFFNALQHLILPALTLAFVHLGI
                     VARQIRSAMLEQLSEDYIRTARASGLPGWYIVLCYALPNALIPSITVLGLALGDLLYG
                     AVLTETVFAWPGMGAWVVTSIQALDFPAVMGFAVVVSFAYVLVNLVVDLLYLWIDPRI
                     GRGGGE"
     gene            10750..11646
                     /locus_tag="OO544_002662"
     CDS             10750..11646
                     /locus_tag="OO544_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HCR6768226.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLML
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLISWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            11643..12629
                     /locus_tag="OO544_002663"
     CDS             11643..12629
                     /locus_tag="OO544_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6768227.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGYVHVLNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGDDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAITLLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGVTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPALTACGDNNQRCACWYPQQEVISI"
     gene            12658..13548
                     /locus_tag="OO544_002664"
     CDS             12658..13548
                     /locus_tag="OO544_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6768228.1"
                     /translation="MNFPARKSWLGKTTEHVHAINGIDLQIRRGETLGIVGESGCGKS
                     TLAQLLMGMLQPSHGQYIRSGSQRIMQMVFQDPLSSLNPRLSVWRIITEPLWIAKRSS
                     EQQRRALAKELAVQVGIRPEYLDRLPHAFSGGQRQRIAIARALSSQPDVIVLDEPTSA
                     LDISVQAQILNLLVTLQENHGLTYVLISHNVSVIRHMSDRVAVMYLGQIVELGDAQQV
                     LTAPAHPYTRLLLDSLPAIDKPLEEEWALCKTDLPGNRTLPQGCFFYERCPLATHGCE
                     VRQSLAIREDGRELRCWRAL"
     gene            complement(13604..14035)
                     /gene="osmC"
                     /locus_tag="OO544_002665"
     CDS             complement(13604..14035)
                     /gene="osmC"
                     /locus_tag="OO544_002665"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HCR6768229.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTQSGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDADFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
BASE COUNT         3577 a         3557 c         3630 g         3640 t
ORIGIN      
        1 acctgataac gtttaacggt aacggtgtcc cgaaacgaac ccgtttcggg ataatttcca
       61 aagtctgttc actggcatta gcaacggaaa atattgttct gaatacgctt cagaacaaaa
      121 caggtgcggt tccgacagga ataccgtttt agggggataa tatggctaca tcagtacaga
      181 caggtaaagc taagcagctc acattactcg gcttttttgc cataacggca tcgatggtaa
      241 tggctgttta tgaataccct accttcgcaa catcgggctt ttcattagtc ttcttcctgc
      301 tattaggcgg gattttatgg tttattcccg tgggactttg tgctgcggaa atggccaccg
      361 tcgacggctg ggaagaaggt ggtgtcttcg cctgggtatc aaatactctg gggccgagat
      421 ggggatttgc agcgatctca tttggctatc tgcaaatcgc cattggtttt attccgatgc
      481 tctatttcgt gttaggggca ctctcctaca tcctgaaatg gccagcgctg aatgaagacc
      541 ccattaccaa aactattgca gcactcatca ttctttgggc gctggcatta acgcagtttg
      601 gtggcacgaa atacacggca cgaattgcta aagttggctt cttcgccggt atcctgttac
      661 ctgcatttat tttgatcgca ttagcggcta tttatctgca ctccggtgcc cccgttgcta
      721 tcgaaatgga ttcaaagacc ttcttccctg acttctctaa agtgggcacc ctggttgtat
      781 ttgttgcctt cattttgagt tatatgggcg tagaagcatc cgcaacccac gtcaatgaaa
      841 tgagcaaccc agggcgcgac tatccgttgg ctatgttact gctgatggta gcggcaatct
      901 gcttaagctc tgttggtggt ttgtctattg cgatggtcat tccgggtaat gaaatcaacc
      961 tctccgcagg ggtaatgcaa acctttaccg ttctgatgtc ccatgtggca ccggaaattg
     1021 agtggacggt tcgcgtgatc tccgcactgc tgttgctggg tgttctggcg gaaatcgcct
     1081 cctggattgt tggtccttct cgcgggatgt atgtcacagc gcagaaaaac ctgctgccag
     1141 cggcattcgc taaaatgaac aaaaatggcg taccggtaac gctggtcatt tcacagctgg
     1201 tgattacttc tatcgcgttg atcatcctca ccaataccgg tggcggtaac aacatgtcct
     1261 tcctgatcgc actggcgctg acggtggtga tttatctgtg tgcttatttc atgctgttta
     1321 ttggctacat tgtgttggtt cttaaacatc ctgacttaaa acgcacattt aatatccctg
     1381 gtggtaaagg ggtgaaactg gtcgtggcta ttgtcggtct gctgacttca attatggcgt
     1441 ttattgtttc cttcctgccg ccggataaca tccagggtga ctctaccgat atgtatgttg
     1501 aattactggt cgttagtttc ctggtggtac ttgccctgcc ctttattctc tatgctgttc
     1561 atgatcgtaa aggcaaagcg aataccggcg tcactctgga gccaatcaac agtcagaacg
     1621 caccaaaagg tcacttcttc ctgcacccgc gtgcacgttc accacactat attgtgatga
     1681 atgacaagaa acactaacca tgtctgaatg gttataagac aagggagcga taattcatcg
     1741 ctcccttttt cgtgctcgct gtcgtattga cttaaccggg ataagtacga gaatgagcgc
     1801 acatctgttt accggaaacc agcatatatg gatatctgct cccgaaacga gaaattagcg
     1861 attagaagac cagcgatact ggttgcactg gcacttttac tgtgtagttg taaaagcacg
     1921 cctccagagt ccatggtgac accaccagca ggttcaaagc caccagccac gacgcaacaa
     1981 tcgtcacaac cgatgcgtgg catctggctg gccacggtgt ctcgcctcga ctggccaccg
     2041 gtttcctcgg ttaacattag taaccccacc agccgggccc gtgtacaaca acaggcgatg
     2101 atcgacaaac tggatcatct gcaacgtctc ggtataaaca cggtcttttt ccaggttaag
     2161 ccggacggta ccgccctgtg gccatcgaaa attttgccat ggtccgatct tatgaccggt
     2221 aagattggtg aaaatccggg ttacgatccg ctgcaattta tgctcgacga agcccacaag
     2281 cgtgggatga aagtacacgc ctggtttaac ccctatcgcg tatcggttaa tacgaagccc
     2341 ggtactatca gggaactgaa tagcactctg tctcaacaac cggcgagcgt ctatgtgcaa
     2401 caccgtgact ggatcagaac gtctggcgat cgctttgtcc tcgacccggg catccctgag
     2461 gttcaggact ggatcacatc aatagtcgca gaagtggttt cccgctatcc ggtagatggc
     2521 gtgcagtttg acgactattt ctatactgag tcaccgggtt cacggctaaa tgataacgaa
     2581 acgtaccgca aatacggagg cgcatttgcg tcaaaagcag actggcggcg caacaatact
     2641 cagcagttaa ttgcaaaagt atcgcacacc attaaaagca ttaagccggg agtcgaattt
     2701 ggcgttagcc cggcaggcgt gtggcgtaac cgatcacacg atccgctcgg ttccgatacc
     2761 cgaggcgcgg cagcctatga cgaatcctac gctgacaccc gtcgatgggt ggaacaagga
     2821 ttgctggatt acattgctcc ccaaatttac tggccgttct cacggagtgc cgcgcgttat
     2881 gacgtgttgg caaaatggtg ggcggatgtc gttaaaccga ctaggacccg cctgtatatc
     2941 ggtatcgcct tctataaagt gggtgaacct tcaaagatag agccagactg gatgattaac
     3001 ggcggcgtac cggaactgaa aaagcagctc gatcttaacg atgcggtacc agaaattagc
     3061 ggcaccatct tgttccgtga ggactatctg aataaaccgc agactcaaca agcggtcagc
     3121 tatctgcaaa gtcgttgggg cagttaaact cccatgctta ctttgtaaca agccggtgct
     3181 ttacccaccg gcttgttaca ccttgtgaaa tatcacacta gtaatcatgc ttacataagt
     3241 caaattaacc acgcaattaa ccacacttag ttaaacagct ataaatccct aataaaaatg
     3301 tatgaatatg aaaaaccata cgatattcgc tgtcaccgaa aaataattac gatgggctat
     3361 actcttctga agaacagtag cgtagattga aactgtccta agtcgcttat ttcttttcag
     3421 tatatcttca tatttcagga gaatatggtg cagttagtca atctcgcacg ttgcgttttg
     3481 attccgtctg atttcccagc cttataaggg tgtgaattat ggagatgtat tttaaaagaa
     3541 tgaaagatga gtggaccgga cttgtcgaac aggcagatcc actcattcgt gctaaagccg
     3601 cggaaattgc cgttgcgcat gctcattatc tgagtatcga gttttatcga attgtccgca
     3661 tcgacccgca tgccgaagaa ttcttgagta atgaacaagt tgagcggcag ttgaaaagtg
     3721 cgatggaacg ctggattatt aacgtgcttt ctgcccaggt tgacgatgtc gaaaggctaa
     3781 tacaaatcca gcataccgtc gcggaagtgc atgcccgcat aggaattccg gtagaaattg
     3841 tcgagatggg gtttcgggtg ctgaaaaaaa tcctctatcc ggtaatcttc tcttcggatt
     3901 attccgccgc agaaaaactt caggtctatc atttctcgat taacagtatt gatatcgcga
     3961 tggaagtgat gacccgcgcg tttaccttta gtgacagtag tgcctcaaag gaagatgaaa
     4021 attatcgtat cttctcgtta ctggaaaacg ccgaagaaga aaaagaacgg caaatagcct
     4081 caatactttc atgggaaata gatattatct ataaaatcct gctggattct gatttaggca
     4141 gtagtttgcc attaagccag gctgattttg gcctgtggtt taaccataaa ggtcgacatt
     4201 attttagtgg cattgccgaa gtaggccata tctcccgtct cattcaggat ttcgacggta
     4261 ttttcaatca aaccatgcgt aacaccagga atttgaataa cagaagtctg cgggtgaaat
     4321 ttttattaca gataagaaat accgtatcgc agattattac cttgctgcgt gaattgtttg
     4381 aagaagtatc gcgccacgaa gtcggtatgg atgtactaac gaaattactt aaccgccgtt
     4441 tcctgccgac tatcttcaaa cgcgaaattg cccatgccaa ccggaccggt acaccgctgt
     4501 cagtgctgat tattgacgtt gataaattca aagagatcaa cgatacgtgg ggccataaca
     4561 ctggcgatga aattctgcgt aaagtctctc aggcctttta tgacaacgtc cacagtagtg
     4621 attatgtttt tcgctacggg ggcgatgaat ttatcattgt tttgactgaa gcttctgaaa
     4681 acgaaacgtt acgtaccgca gaacgtattc gcagtcgggt ggagaaaacc aaactgaaag
     4741 ccgcaaacgg cgaggatatt gccctctcac tttccatcgg tgccgccatg tttaatggtc
     4801 atcctgacta tgagcgcctc attcaaatag ccgatgaagc tctgtatatc gccaaaagac
     4861 gaggtagaaa ccgtgttgaa ctctggaaag ccagtcttta gatgcgccag gatgcagagg
     4921 taatcatgaa gctaaccgat gcggaaaatg ccgccgatgg catttttttc cccgcccttg
     4981 aacaaaatat gatgggcgcg gtgttaatta acgaaaatga tgaagtgatg tttttcaacc
     5041 ccgccgcaga gaagctctgg gggtacaaac gtgaagaagt cattggcaat aacattgata
     5101 tgctgattcc gcgggatttg cgtcctgcgc atcctgaata cattcgtcat aaccgtgaag
     5161 gtggtaaagc gcgcgtcgag gggatgagtc gggagctgca gctggagaaa aaagacggca
     5221 gtaaaatctg gacccgtttt gcgctatcga aagtgagcgc cgaggggaaa gtttattacc
     5281 tggcgctggt acgggatgcc agcgtagaaa tggcgcaaaa agaacagacc cgacaattga
     5341 ttattgccgt tgaccatctc gaccgaccgg tgattgtcct cgatccggaa cgccatattg
     5401 tgcagtgcaa tcgcgcattt accgaaatgt ttggttactg cattagcgaa gccagcggta
     5461 tgcagcccga tacactcctg aacattcctg aattccctgc cgataaccgc attcgtttac
     5521 aacagttgct atggaaaacc gcccgcgatc aggacgaatt tctgctgttg acacgcaccg
     5581 gtgaaaaaat ctggattaaa gcctctatca gcccggttta tgacgtgctc gcgcatctgc
     5641 agaacctggt aatgaccttc tcggatatca ccgaagaacg gcagatccgc cagcttgaag
     5701 gcaatattct cgccgccatg tgcagcagcc cgccatttca tgaaatgggg gaaatcattt
     5761 gtcgtaacat cgaatctgtt ctcaacgaat cgcatgtttc gctgttcgca ctgcgcaacg
     5821 ggatgccgat acactgggcg tcatcttcgc acggtgcaga agttcaaaat gcgcaaagct
     5881 ggtcagcgac cattcgtcag cgtgatggtg cgcctgcggg gatcctgcaa attaaaacct
     5941 cgtcaggagc agaaaccagc gcctttatcg aacgcgtggc agatatcagc cagcatatgg
     6001 ccgcgctggc gctggaacag gaaaaaagcc gtcagcatat tgaacaactc atccaatttg
     6061 atccgatgac cggtctgcca aatcgcaata acctgcacaa ttacctcgat gaccttgtcg
     6121 acaaagccgt ctctccggtg gtgtatctca tcggtgttga ccatattcag gatgtgattg
     6181 atagccttgg ctatgcgtgg gccgatcagg cattgctgga agtggtcaat cgctttcgtg
     6241 aaaaactcaa accggatcag tatctctgtc gtatcgaagg tacgcagttt gtcctcgtga
     6301 gcctggaaaa cgacgtcagt aacattaccc aaatcgccga tgagctacgg aatgtggtca
     6361 gcaagccgat aatgattgac gataaaccat tcccgctaac cttgagtatt ggcatcagct
     6421 acgacgtggg taaaaaccgc gattacctgc tctccactgc tcacaatgca atggattata
     6481 ttcgcaagaa tggcggtaac ggctggcagt tcttcagccc ggcgatgaac gaaatggtaa
     6541 aagagcgttt ggttttaggc gcagcgctga aagaagcgat tagcaataac caactgaaac
     6601 tggtttacca gccgcaaatc ttcgcagaaa cgggtgaact gtacggcatc gaagcccttg
     6661 ctcgctggca cgatccccag catggtcatg tacccccttc acggtttatt cctctcgcag
     6721 aagagattgg tgaaatcgaa aatattgggc gctgggtcat cgcggaagct tgccgtcagt
     6781 tagcagaatg gcgtagccag aatattcata tcccggcgtt atccgtgaac ttgtcggcgc
     6841 tgcactttcg cagtaatcag ctgcctaatc aggtgtctga tgcaatgcaa gcctggggta
     6901 ttgacggcca ccagctgacg gtagaaatca cggaaagcat gatgatggaa cacgataccg
     6961 aaatctttaa gcgcattcag atcctgcgcg atatgggcgt gggcttatcg gtagatgatt
     7021 ttggcacggg cttttccgga ttatcccgct tagtcagtct tccggtaacg gaaatcaaaa
     7081 ttgacaaaag ttttgtcgat cgttgtctga cggaaaaacg catccttgcc ttacttgaag
     7141 ccattaccag cattgggcaa agcctcaatt taaccgtcgt ggcggaaggc gtcgaaacca
     7201 aagagcaatt tgagatgcta cgcaagatcc actgtcgcgt tattcaggga tatttctttt
     7261 cccgccccct acccgccgag gaaattccag gctggatgag cagcgtgtta ccgctgaaaa
     7321 tctgacaaat tcctctcgcc cgcactcgcg ggttttcatt taacgtgacg ctgtcactta
     7381 aatactgtga tttcagctac gattccggga gattccttcc ttaacttccc ctcttgcacc
     7441 aaaactgcac tacgctgcac aaatgttgat caaaagttaa aaaatataac tttaagccag
     7501 cattctggcg cttatcccgg catggcatga gatctgcata agcggaaagc gcagcaattt
     7561 ttgtcttaat acaggaaccg tttatgtcgg ataccaccga actggttgat ttagccgtga
     7621 tcttccctga tctggagatc gaattgaaat acgcctgcgc tgataacatc acaggtaaag
     7681 ctatttacca gcaagcgcgt tgtctgttac acaaggatgc gattaccgcg ctggcgaaaa
     7741 gtatcagtat cgcccagctg tcaggcttac aactggtgat ttacgatgcg tatcgcccac
     7801 aacaagcaca ggcgatgttg tggcaagcct gcccagaccc gcaatatgtt gttgatgtga
     7861 cggtcggttc taatcacagc cgtggcacgg cgatcgacct gacgcttcgt gatgagcacg
     7921 ggaacatcct ggatatgggc gcagggttcg atgaaatgca cgagcgctcc catgcctatc
     7981 acccttccgt cccgcccgcc gctcagcgca atcgcctgtt gctgaatgcg ataatgactg
     8041 gtggcggttt tgtcggtatc tccagcgaat ggtggcactt cgaattaccc caggcagcga
     8101 gttaccctct gctcgctgat caattctctt gttttatatc ccctggcaca cagcacgtca
     8161 gttaatcagg aaactgtcat gaagagatcg atatcgtttc gtcccacatt gctcgcgctc
     8221 gtccttgcca caactttccc ggttgcgcac gccgccgtac cgaaagatat gctggtgatt
     8281 ggtaaggccg ccgatccaca aaccctcgac ccggcggtaa caatagataa taacgactgg
     8341 acagtgacct acccgtctta tcagcggctg gttcagtaca aaacggacgg tgataaaggc
     8401 tcaaccgacg ttgaaggcga tctggcaagt agctggaaag cgtctgacga tcaaaaagag
     8461 tggacgttca ccctgaagga caatgctaaa tttgccgatg gcacacctgt cactgccgaa
     8521 gcagtaaaac tctcttttga gcggttacta aaaatcggcc aggggccagc agaagcattt
     8581 cccaaagatt taaagattga tactcccgac gaacatacgg tgaagtttac ccttagccaa
     8641 ccattcgcac cgttcctcta cacgctagcg aatgacggtg catccattat caatccggcg
     8701 gtcttaaagg aacatgcagc ggatgatgct cgcggcttcc tcgcgcaaaa taccgccggt
     8761 tccggaccat ttatgctgaa aagcgggcaa aaaggtcagc aattagttct ggtgccaaat
     8821 ccgcattacc ccggcaataa accgaacttt aagcgagtat cggtaaaaat tatcggtgaa
     8881 agtgcctccc gtcgcctgca gctctcccgt ggcgacattg acattgccga tgcgctgccg
     8941 gtggatcaac tcaacgccct gaagcaggaa aataaagtca atgtggcaga gtatccgtca
     9001 ctgcgcgtta cctatctgta tctgaataac agcaaagcgc ctcttaatca ggcggatctg
     9061 cgtcgggcca tttcctggtc taccgattat cagggcatgg ttaacggcat tctgagtggt
     9121 aacggaaaac agatgcgcgg cccgattccg gaaggcatgt ggggctacga tgcgacggca
     9181 atgcaataca accatgacga aacgaaagcc aaagccgaat gggataaagt gacgagcaaa
     9241 cccaccagcc tgacgtttct ctactccgat aacgatccga actgggagcc tattgctctg
     9301 gcgacacaat ccagtctcaa caagctgggc atcaatgtga agctggaaaa gctggcgaac
     9361 gccaccatgc gcgacagagt gggtaaaggt gattacgaca ttgcgattgg caactggagt
     9421 ccggattttg ccgacccgta tatgtttatg aattactggt ttgagtcaga caaaaaaggt
     9481 ctgccgggta accgctcgtt ctatgaaaac agtgaggtcg ataagttact gcgcaatgcg
     9541 cttgcgacca ccgaccagac gcagcgtacc cgggactacc agcaggcaca gaaaatcgtc
     9601 attgatgacg ctgcttatgt gtacctgttc cagaaaaact accaactggc gatgaacaaa
     9661 gaggtgaaag gctttgtgtt caatcccatg ctggaacagg tcttcaatat caataccatg
     9721 agtaaataac gtgaccttct ggagtatttt acgccaacgc tgctgggggc tggtgctcgt
     9781 ggtggcgggc gtctgcgtga ttacgtttat tatctcgcat ctgatccctg gcgatccggc
     9841 gcggttactg gcgggtgacc gcgccagcga tgctatcgtg gaaaatattc gccagcaact
     9901 gggactggac cagccactgt atgtacagtt ttaccgctac ttcagcgacc tgtttcatgg
     9961 tgacctggga acatccattc gtaccgggcg tccggtgctg gaagagttgc gtatattttt
    10021 cccggcgacg ctggaactgg ctttttgtgc cctgctgctg gcactcctga ttggcatccc
    10081 gctgggcata ctctctgcag tctggcgaaa tcgctggctg gatcatctgg tgcgaataat
    10141 ggccattacc ggaatctcca cacctgcgtt ctggcttgga ctgggcgtca ttgtgctgtt
    10201 ttatggtcat ctgcaaattc ttcccggcgg cggaaggctt gatgactggc tggatccacc
    10261 aacgcacgtt accggctttt atctgctcga tgcgctgctt gaaggcaacg gtgaagtctt
    10321 cttcaatgcg ttgcaacatc tcatcttacc ggcattaacg ctggcgttcg ttcacctggg
    10381 aattgtcgct cgccagatcc gctcagcgat gctggaacaa ttgagtgaag actacattcg
    10441 taccgcccgg gccagcggct tgcccggctg gtatatcgtt ttatgttatg cgctacccaa
    10501 tgcgttgatc ccatcgatta ccgtattggg tctggcgctg ggcgatttgt tgtatggcgc
    10561 agtgctcacc gaaaccgttt ttgcctggcc cggaatgggt gcatgggtag taacatcaat
    10621 acaggcgctc gacttcccgg cagtgatggg ctttgccgtc gtggtttcat ttgcttatgt
    10681 gctggtcaac ctggtggtgg atttgctcta tttgtggatt gatccgcgta tcggacgtgg
    10741 aggtggtgaa tgatgctaag cgaggaaacg tccgccgtac gcccacaaaa acaaacgcga
    10801 tttaacggtg caaaactggt atggatgctg aaaggcagtc cgctcaccgt gaccggcgca
    10861 gtcatcattg tattaatgct attgatgatg attttttcac cgtggcttgc gacgcatgat
    10921 cccaacgcca ttgatttaac cgcccgcctt ttgccgcctt ctgcggcgca ctggtttggc
    10981 accgatgaag tgggacgcga tctgttcagc cgcgtactgg tcggcagtca gcaatcaatt
    11041 ctcgccggat tagtcgtggt tgccattgcg ggtatgattg gttcgctact cggatgtcta
    11101 tccggtgtgc ttggcggacg cgcagacgcc atcatcatgc gcatcatgga cattatgctg
    11161 tcgattcctt cgttggtact gacaatggca ctggctgccg ctctcgggcc gagtttattt
    11221 aacgccatgc tggcgattgc tattgtgcga attccctttt atgtgcgcct ggcgcggggg
    11281 caagcattag ttgtacgcca gtatacctat gttcaggcgg cgaaaacctt tggtgcgtcg
    11341 cgttggcatc tgatcagctg gcatatttta cgtaactccc tgccgccgct gatcgtgcag
    11401 gcatcgctgg atatcggtag cgcgatttta atggccgcca cgttgggatt tattggcctg
    11461 ggtgctcaac aaccgagtgc tgaatggggc gcgatggtag ctaatggtcg caactatgta
    11521 ctcgatcagt ggtggtattg cgcatttccg ggggcagcga ttttgcttac cgccgtcggg
    11581 tttaatctct ttggtgatgg tattcgcgat ctgcttgacc cgaaagcagg aggaaagcag
    11641 tcatgaccca acccgttctg gacattcaac agctgcattt gagtttcccc ggttttaacg
    11701 gttatgttca cgtgctcaac aatgtgtcct tgcagattaa ccgcggtgaa attgtcggtc
    11761 tggtgggaga atccggctca ggtaaatcag tcaccgcaat gctgattatg cgtctgctac
    11821 cgacgggcag ttattgcgta catcgggggc agatttcact gctgggagac gatgttctta
    11881 acgctcggga gaaacagctt cgtcagtggc gcggcgcacg agtggcgatg atctttcagg
    11941 aaccgatgac cgccctcaat ccgacacgtc gaataggtct tcagatgatg gacgtgatcc
    12001 gccatcatca accaataagt cgtcgggaag ccagagctaa agcgattacc ctgctggaag
    12061 agatgcaaat cccggatgcc gtggaagtta tgtcgcgcta tccgtttgag ctttcaggtg
    12121 gtatgcgcca gcgggtaatg attgcgctgg cattctcctg cgagccgcaa ttgattattg
    12181 ccgacgaacc gactacggcg ctggacgtca cggtacagtt gcaggtactg cgtttgctta
    12241 aacataaagc tcgcgccagt ggaactgcgg tactgttcat cagccatgat atggccgtgg
    12301 tgtcgcaact gtgcgatagc gtttacgtga tgtatgccgg aagcgtaatt gaaagcggcg
    12361 tgacggcaga cgttatccat catccccggc atccgtatac cattggtttg ctgcaatgcg
    12421 caccagaaca tggagtacca cgccagccat tacccgccat tccagggacg gtaccaaacc
    12481 tcacccattt gcctgacggc tgcgcttttc gcgatcgttg ctatgcggca ggtgcacagt
    12541 gtgaaaacgt cccggcgctg acagcgtgtg gtgacaacaa ccagcgctgc gcctgttggt
    12601 atccgcagca ggaggtcata agtatctgac acgttattaa cgttacgcga cgtccatatc
    12661 aatttcccgg cccgtaaaag ctggcttggt aaaactacgg aacatgttca tgcgattaat
    12721 ggtattgatt tacagatccg ccgtggtgaa accttaggga tcgtcggtga gtcaggctgc
    12781 ggcaaaagca ccctcgcaca gcttttaatg ggtatgctgc aaccgagcca cgggcaatac
    12841 atccgttcag gatcacaacg cattatgcag atggtgtttc aggacccgct ctcttcgctt
    12901 aatccgcgct tatcggtgtg gcgcatcatc acagaaccgc tctggatagc taagcgtagt
    12961 agtgaacaac agcggcgagc gttggcaaag gagttggctg tgcaggtggg tattcgtccg
    13021 gagtatctcg accgcctgcc tcatgcgttc tccggcgggc agcggcaacg catcgccatt
    13081 gccagagcac tctcttcgca gcctgacgtg attgtgcttg atgagccaac ctcggcgctg
    13141 gatatctccg tgcaggcgca gatcctcaat ttactggtaa cgctacagga aaatcacggg
    13201 ctgacttatg tgctgatttc acacaatgtc tcggtgatac gtcatatgag cgatcgggtg
    13261 gcggtgatgt atctcgggca gattgtagaa ctgggcgacg cgcagcaggt gctgacggca
    13321 cctgcacatc catacacccg attattgctg gattccctcc ccgccattga taaaccgctg
    13381 gaggaagaat gggcattatg taaaacggat ctgccgggaa accgcacgtt gccacaaggc
    13441 tgttttttct acgaacgttg ccctctggca acccacggat gtgaagtccg gcaatcatta
    13501 gcaataaggg aggacggacg tgagctccgg tgctggcggg cgctgtagag atgctaacaa
    13561 cgcatcaggc attcacgcgc ctgacgcgtc atccggcaat gctttacgat ttcaactggt
    13621 aatccagcgt aatttccgct ttcagtacct gagaaaccgg gcatcctgct tttgctttct
    13681 ggatgatgcc gtcaaaggta gaggcatcaa tacccggcac cgcaacttca ctcttcagtg
    13741 cgattttcgt aatcgcaaaa tcggcatcca ctttatccag cgacacatcg gcggtggtat
    13801 caatcgatgt tggcgtgaat cccgcttccc ccagcattaa tgaaagcgcc attgagaaac
    13861 atgcggcatg cgctgcgcca atcagttctt cagggttggt tcctttttcg ccttcaaaac
    13921 gcgtgttaaa tccatacggc tgttggttca gcacgccact ctgggtggat actgttccct
    13981 tcccgcgttt gatatcgcct tcccagtgtg cctgaccttt cttatggatt gtcattgttg
    14041 ctctcctgtg ggcttaaaaa ggaaattata gactacgtga gaaacagcac gaggataagc
    14101 aggatattcc gaataaaata cggcgaaaat attacttctg cacttaaatt aaattcccac
    14161 aatagataac cgggaatata tatctcaaga gatattgcat caatatcaat caactcacca
    14221 caataataat acatatcaat atcatggagt taaaatataa aaatcatcaa ccaggactaa
    14281 tcttaacaac gaagcggcaa atatttgcca tgctgaatgt gctttataaa tctgcgatcc
    14341 gtagcagaca ccataaatac acagacacgg agaatcacta tgtttactta ttatcaggca
    14401 gaaa
//
LOCUS       DAJTZL010000102        14369 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.102, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000102 DAJTZL010000000
VERSION     DAJTZL010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14369
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.102"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..696)
                     /locus_tag="OO544_002666"
     CDS             complement(<1..696)
                     /locus_tag="OO544_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR6768230.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHVQPNGEESYHLTREGGHSHRRILHAADATGREVQSTLVSKAQNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAG"
     gene            952..1107
                     /gene="rseD"
                     /locus_tag="OO544_002667"
     CDS             952..1107
                     /gene="rseD"
                     /locus_tag="OO544_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HCR6768231.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            1104..1679
                     /gene="rpoE"
                     /locus_tag="OO544_002668"
     CDS             1104..1679
                     /gene="rpoE"
                     /locus_tag="OO544_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HCR6768232.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            1712..2362
                     /gene="rseA"
                     /locus_tag="OO544_002669"
     CDS             1712..2362
                     /gene="rseA"
                     /locus_tag="OO544_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HCR6768233.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWHKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGLQTLGTQSQ"
     gene            2362..3318
                     /gene="rseB"
                     /locus_tag="OO544_002670"
     CDS             2362..3318
                     /gene="rseB"
                     /locus_tag="OO544_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HCR6768234.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVISFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            3315..3794
                     /gene="rseC"
                     /locus_tag="OO544_002671"
     CDS             3315..3794
                     /gene="rseC"
                     /locus_tag="OO544_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HCR6768235.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAILGGIGGFLIARGYSRKFAARAEWQPIILSVALPPDLVRFETSSEDASQ"
     gene            3992..5791
                     /gene="lepA"
                     /locus_tag="OO544_002672"
     CDS             3992..5791
                     /gene="lepA"
                     /locus_tag="OO544_002672"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HCR6768236.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGSLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            5807..6781
                     /gene="lepB"
                     /locus_tag="OO544_002673"
     CDS             5807..6781
                     /gene="lepB"
                     /locus_tag="OO544_002673"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCR6768237.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKLGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            7054..7734
                     /gene="rnc"
                     /locus_tag="OO544_002674"
     CDS             7054..7734
                     /gene="rnc"
                     /locus_tag="OO544_002674"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HCR6768238.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            7731..8636
                     /gene="era"
                     /locus_tag="OO544_002675"
     CDS             7731..8636
                     /gene="era"
                     /locus_tag="OO544_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HCR6768239.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLRDGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            8648..9376
                     /gene="recO"
                     /locus_tag="OO544_002676"
     CDS             8648..9376
                     /gene="recO"
                     /locus_tag="OO544_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HCR6768240.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGATGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            9388..10119
                     /gene="pdxJ"
                     /locus_tag="OO544_002677"
     CDS             9388..10119
                     /gene="pdxJ"
                     /locus_tag="OO544_002677"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HCR6768241.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKMRDACKRLTDAGIQISLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELVRIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            10119..10499
                     /locus_tag="OO544_002678"
     CDS             10119..10499
                     /locus_tag="OO544_002678"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HCR6768242.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            10721..10801
                     /gene="shoB"
                     /locus_tag="OO544_002679"
     CDS             10721..10801
                     /gene="shoB"
                     /locus_tag="OO544_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HCR6768243.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(10995..11255)
                     /gene="yfhL"
                     /locus_tag="OO544_002680"
     CDS             complement(10995..11255)
                     /gene="yfhL"
                     /locus_tag="OO544_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HCR6768244.1"
                     /translation="MSLLITKRCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            11470..12660
                     /locus_tag="OO544_002681"
                     /pseudo
     CDS             11470..12660
                     /locus_tag="OO544_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(12845..14032)
                     /locus_tag="OO544_002682"
     CDS             complement(12845..14032)
                     /locus_tag="OO544_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HCR6768245.1"
                     /translation="MLPRFADIFQQGNHWLNWLEKQPERAVRPVVIESVTKIMACGTT
                     LMGYTQWCCSSPDCCHTKKVCFRCKSRSCPHCGVKAGAQWIQYLLSLVPDCPWQHIVF
                     TLPCQYWSLVFHNRWLLTEMSRIAADVILEICHQADVEPGIFTVIHTWGRDQQWHPHI
                     HLSTTAGGVTSGHTWKNLHFYARKVMSMWRYRITRLLSRKYPDLVMPDALAAEGSSKR
                     EWNRFLDTHYRRGWNVNVSRVMDNAPHVAVYFGSYLKKPPVPMSRLEHYAGQDEIGLR
                     YNSHRTKREEYLLMSGDEFMERFSWHVADKGFRMVRYYGFLSPAKRRLLEEVVYIITE
                     TVRKTAMQITWRGMYQRLLKVDPLKCVLCGSQMRFTGLKRGYRLAEQVLMHEPLARMR
                     WCG"
     gene            complement(14032..>14369)
                     /locus_tag="OO544_002683"
     CDS             complement(14032..>14369)
                     /locus_tag="OO544_002683"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6768246.1"
                     /translation="KPASQRSKLPRYVVKLHEDDFFDEEDAEVLRFDSFDDAVECCAD
                     LNIPFFVDAGNKKLVFWFVRVDDEGYPEIARCTEREFATILSGISAGGMYCPECGTVH
                     WPDGVAPPF"
BASE COUNT         3621 a         3606 c         3708 g         3434 t
ORIGIN      
        1 gcccgcgcgc cacgccatag caatgccatc gccagaagaa atatccggat tggtggtgta
       61 ctgatacacc ttcgacgcac cgccggttgc cagtaccact gcttttgcgt ggcaggtttc
      121 caccgtttct ttatttcggt tccataccca cgcgcctaca acccgtcgcg tgcccggcag
      181 gccaattttg tcagagacaa tcagatcaac tgcgttgctg cgctccagca cacgaatatt
      241 cggatggttc tgcgccttgc tcaccagcgt ggattgtact tctctaccgg tggcgtcggc
      301 ggcatgaaga atacgacggt gactatgtcc accttcacgg gtcagatggt agctttcttc
      361 gccattcggt tgaacgtggg tatcaaacaa aaccccctgg tcaattaacc attgcacaca
      421 ggaacgagca ttgctggcga caaattcaac tgcatggcga tcgcaaatac cagccccggc
      481 aatcaatgtg tcttccacat gcgagtcaat gctgtcagtt tcatcaaaca cggcggcaat
      541 accgccctgg gcataaaatg ttgaaccttc cgttaccggg cctttactta gaacgatgac
      601 ctgatgctgg tcagccaggc gtagcgccag tgaaagtccg gctgcgccgc taccaataat
      661 caacacgtca catgaatgtt cagggagagt attcattttc tttgtttaat ttactaaaca
      721 ttgtttggtc agcatagcat catgttgtgc gaataaacac ctgctatttt aatatttgtt
      781 acagtggcta aacacgttga cgcagggcgg cgagaaaaag agaagttact ggctggtgga
      841 ggattaggtg gtgaaataaa aaggccgttg ggttactctt caggcagtta aatgggcatt
      901 tctacacaga taatgcgatg ttcagattct gtagacttat aatgatagat aatgatccgt
      961 ctacagcatg acaaacaaaa acagatgcgt tacggaactt tacaaaaacg agacactcta
     1021 accctttgct tgctcaaatt gcagctaatg gagtggcgtt tcgatagcgc gtggaaattt
     1081 ggtttgggga gactttacct cggatgagcg agcagttaac ggaccaggtc ctggttgaac
     1141 gggtccagaa gggagatcag aaagccttta acttactggt agtgcgctat cagcataaag
     1201 tggcgagtct ggtttcccgc tatgtgccgt cgggtgatgt tcccgatgtg gtacaagaag
     1261 cttttattaa agcctatcgt gcgctggatt cgttccgggg agatagcgct ttttatacat
     1321 ggctgtatcg gattgctgta aatacagcga aaaattacct ggttgctcag gggcgtcgtc
     1381 caccttccag tgatgtggat gccattgaag ctgaaaactt cgaaagtggc ggcgcgttga
     1441 aagaaatttc gaaccctgag aacttaatgt tgtcagaaga actgagacag atagttttcc
     1501 gaactattga gtccctcccg gaagatttgc gcatggcaat aaccttgcgg gagctggatg
     1561 gcctgagcta tgaagagata gccgctatca tggattgtcc ggtaggtacg gtgcgttcac
     1621 gtatcttccg agcgagggaa gctattgata acaaagttca accgcttatc aggcgttgac
     1681 gatagcggga tactggataa gggtattagg catgcagaaa gaacaacttt ccgctttaat
     1741 ggatggcgaa acgctggata gtgagctgct taacgaactg gctcataacc cagaaatgca
     1801 gaaaacctgg gaaagctatc acttaatccg tgattcaatg cggggtgata ctcccgaggt
     1861 gctccatttc gatatctctt cacgcgtgat ggccgccatt gaagaagagc cagtacgtca
     1921 accggcgaca ttgatcccgg aagcccagcc tgcgccgcat caatggcaca aaatgccatt
     1981 ctggcagaaa gtacgtccgt gggcggcaca gcttacccaa atgggcgtag cagcatgcgt
     2041 atcgcttgca gttatcgttg gcgtccagca ctataatgga caatctgaaa cgtcccagca
     2101 gcccgaaacg ccggtattta atacactgcc gatgatgggt aaagccagcc cggtaagcct
     2161 gggagtacct tctgaagcga ccgcaaacaa tggtcaacag cagcaggtac aggagcagcg
     2221 tcgtcgcatt aatgcaatgt tgcaggatta cgaactgcaa cgccgactcc actctgaaca
     2281 gcttcagttt gagcaggcgc aaacccagca agccgctgta caggtgccag gacttcaaac
     2341 tttaggaacg caatcgcagt aatgaagcaa ctttggtttg ccatgtcatt agtgacaggt
     2401 agcctgttat tctctgctaa cgcctcggcc actcccgcgt ccggggcgtt attacagcag
     2461 atgaacctgg ccagtcagtc actgaattac gagctgtcat tcatcagcat caataaacag
     2521 ggtgttgagt ctctgcgtta tcgacatgca cgcctcgata accgtcctct tgcacaattg
     2581 ttgcaaatgg atggcccgcg ccgggaagtg gtacagcgcg gcaatgaaat cagctatttt
     2641 gaaccgggac ttgaaccgtt cacgcttaat ggcgattaca ttgttgattc tctgccatcg
     2701 cttatctata ccgatttcaa acgcctttct ccttactacg actttatctc cgtcgggcgc
     2761 acgcgtattg ctgatcgtct ttgcgaagtc attcgcgtgg ttgcccgaga tggtacacgc
     2821 tacagctaca tcgtgtggat ggacactgaa tcgaaattac cgatgagggt tgatctcctt
     2881 gatcgcgatg gtgaaacgct ggaacaattt cgcgtgattt cttttaacgt caatcaggat
     2941 atcagcagca gtatgcagac gctggcgaag gcaaatttgc cgccgttgct ttctgttcct
     3001 gtaggtgaaa aagctaaatt cagctggacg ccaacctggt tgccacaggg ttttagcgaa
     3061 gtttccagta gtcgacgtcc gctaccgacg atggacaaca tgcctatcga atcacgtctc
     3121 tattccgacg gattattcag cttctcggta aacgttaacc gcgctacgcc atcgagcacc
     3181 gatcagatgt tgcgcaccgg acgcagaacc gtcagtacaa gcgtacgtga taacgccgaa
     3241 atcaccattg tcggtgaact gccgccgcaa acggcgaaac gcattgccga gaatattaag
     3301 ttcggggcag cgcaatgatc aaagagtggg ctaccgtcgt ctcctggcaa aacgggcagg
     3361 cgctggtcag ttgtgatgtt aaagcctcat gcagcagctg cgcttcacgc gccggttgcg
     3421 gtagccgcgt gttgaataaa cttggcccgc aaaccacgca taccattgtc gtaccctgtg
     3481 atgaaccgtt agtgccgggg caaaaagtgg aattggggat cgccgaaggc agcctgctta
     3541 gctccgcatt actggtttat atgtcgccgc tggtgggatt attccttatc gcttcgctat
     3601 ttcagctact ctttgcttct gacgttgcag cattatgtgg tgcgatcctt ggcggtattg
     3661 gcgggtttct gattgcgcgc ggttactcgc gtaagtttgc tgcccgggca gaatggcaac
     3721 cgatcatatt aagcgtggca ttgccgcctg accttgtgcg atttgaaaca tcttcggaag
     3781 acgcgagcca gtgatttcac tggctttgtt gcattttgca taacaaatgc gaggacgttt
     3841 ccagatacca caatccatac ggtatgaaca tttcctcgcc tcaatgttgt agtgtagaat
     3901 gcggcgtttc tattaataca gacgttaagc tcagaacagc gacttctaaa agcctggtta
     3961 accgggcatt aaggcacaat aatcatactt tatgaagaat atacgtaact tttcgatcat
     4021 agctcacatt gaccacggta aatcgacgct gtctgaccgt attatccaga tctgcggtgg
     4081 cctgtctgac cgtgaaatgg aggcgcaggt tctcgattcc atggatcttg agcgtgagcg
     4141 tggcattacc atcaaagcgc aaagcgtgac gctggactac aaagcgtctg acggcgaaac
     4201 ctatcagctt aactttatcg acaccccggg ccacgtagac ttctcctatg aagtttcccg
     4261 ttcgctggct gcctgtgaag gtgcattgct ggtggtcgac gccgggcagg gcgtggaagc
     4321 gcaaaccctg gcaaactgct acaccgccat ggaaatggat ctcgaagtgg tgccagtact
     4381 gaacaagatt gacctgccgg cagccgatcc tgaacgcgtg gcggaagaaa ttgaagatat
     4441 cgtcggcatc gacgccactg acgcggtgcg ctgttcagcg aaaaccggcg ttggcgtgca
     4501 ggacgttctc gaacgtctgg tgcgcgacat tccgccgccg gaaggcgatc cggaaggctc
     4561 gttgcaggca ctaattatcg actcatggtt cgacaactac ctgggcgttg tttcacttat
     4621 ccgtattaaa aacggcaccc tgcgtaaggg cgacaaagtg aaagtcatga gtaccgggca
     4681 gacctataac gccgaccgtc tgggcatctt cacgccgaaa caggttgacc gcactgaact
     4741 gaaatgtggc gaagtaggct ggctggtatg tgcgattaaa gatatccatg gcgctccagt
     4801 cggcgatacc ttaacgctgg cgcgtaatcc ggcagaaaag gcgctgcctg gctttaagaa
     4861 agtcaaaccg caggtatacg ccggtctgtt cccggtaagt tccgacgact atgaagcctt
     4921 ccgtgacgcg ctgggtaaac tcagcctgaa tgatgcctca ctgttctatg agccggaaag
     4981 ctccagcgcg ctgggctttg gtttccgctg cggcttcctt ggcctgctgc acatggagat
     5041 catccaggaa cgtctggaac gtgaatacga tctggatctg atcaccactg cgccgaccgt
     5101 agtgtatgaa gttgaaacca cgtcaaggga agttatctac gtcgacagcc catccaagct
     5161 gcctgcggta aataacattt acgaactgcg cgagccgatt gcagagtgtc acatgctgct
     5221 gccgcaggca tatctcggca acgttattac gctgtgcgta gaaaaacgcg gcgtgcagac
     5281 caatatggtt taccacggta atcaggtggc gctgacgtac gagatcccga tggcggaagt
     5341 ggtgctcgac ttcttcgatc gcctgaaatc tacctcgcgt ggttatgcgt ctctggatta
     5401 caacttcaaa cgcttccagg cgtccgacat ggtacgtgta gacgtattaa tcaacggtga
     5461 acgtgttgat gcgttggcgc tgatcaccca ccgtgataat tcgcaaaacc gcggtcgcga
     5521 gttggtggag aagatgaaag atctgatccc acgccagcag tttgatatcg ccattcaggc
     5581 agcgattggt acgcacatta ttgcgcgatc caccgtgaaa cagctgcgta aaaacgtact
     5641 ggctaaatgt tatggcggcg atatcagccg taagaaaaag ctgctgcaga agcagaaaga
     5701 aggtaagaaa cgcatgaagc agatcggtaa cgtcgagctg ccgcaggaag cgttcctcgc
     5761 cattctgcac gtcggcaaag acaacaaata acccttagga gttggcatgg cgaatatgtt
     5821 tgccctgatt ctggtgattg ccacactggt gacgggcatt ttatggtgcg tggataaatt
     5881 ctttttcgca cctaaacggc gggaacgtca ggcagcggcg caggcggctg ccggtgactc
     5941 actggataaa gcaacgttga aaaaggttgc accgaagctt ggctggctgg aaaccggagc
     6001 ttctgttttt ccggtgctgg ctatcgtatt gattgtacgt tcgtttattt atgaaccgtt
     6061 ccagatcccg tcaggttcga tgatgccgac tctgttaatc ggtgatttta ttctggtaga
     6121 gaagtttgct tatggcatta aagatcctat ctaccagaaa acgctgatcg aaaccggtca
     6181 tccgaaacgc ggcgatatcg tggtctttaa atatccggaa gatccaaagc ttgattacat
     6241 caagcgcgcg gtgggtttac cgggcgataa agtcacttac gatccggtct caaaagagct
     6301 gacgattcaa ccgggatgca gttccggcca ggcgtgtgaa aacgcgctgc cggtcaccta
     6361 ctcaaacgtg gaaccgagcg atttcgttca gaccttctca cgccgtaatg gtggggaagc
     6421 gaccagcgga ttctttgaag tgccgaaaaa cgaaaccaaa gaaaatggaa ttcgtctttc
     6481 cgagcgtaaa gagacactgg gtgatgtgac gcaccgaatt ctgacagtgc cgattgcgca
     6541 ggaccaggtg gggatgtatt accagcagcc agggcaacaa ctggcaacct ggattgttcc
     6601 gccgggacaa tacttcatga tgggcgacaa ccgcgacaac agcgcggaca gccgttactg
     6661 gggctttgtg cctgaagcga atctggtcgg tcgggcaacg gctatctgga tgagtttcga
     6721 taagcaagaa ggcgaatggc cgacaggtgt gcgcttaagt cgcattggcg gcatccatta
     6781 atagcccatc ttcgttcacg ttgtcgccgt tatggcgaca acgtgaatta tttatgagat
     6841 aaatctcccg tggctaacga catcccccgt cgttgtgtat agaatattcc cccgaagttt
     6901 aaggttggca cctccaggtt gccacggcac acgaaacagc gttggtcccc atataccggt
     6961 aaactgaaac tgcagcgaag cagttagcag agccatgtat atcaggtctg tttcgtgtgc
     7021 tgaattgttg acgcatttat ttattggtat cgcatgaacc ccatcgtaat taatcggctt
     7081 caacggaagc tgggctacac ttttaatcat caggaactgt tgcagcaggc attaactcat
     7141 cgtagtgcca gcagcaaaca taacgagcgt ttagaatttt taggcgactc tattctgagc
     7201 tacgttatcg ccaatgcgct ttatcaccgt ttccctcgtg tggatgaagg cgatatgagc
     7261 cggatgcgcg ccacgctggt ccgtggcaat acgctggcgg aactggcgcg cgaatttgag
     7321 ttaggcgagt gcttacgttt agggccaggt gaacttaaaa gcggtggatt tcgtcgtgag
     7381 tcaattctcg ccgataccgt cgaagcatta attggtggcg tattcctcga cagtgatatt
     7441 caaaccgtcg agaaattaat cctcaactgg tatcaaactc gtttggacga aattagccca
     7501 ggcgataaac aaaaagatcc gaaaacgcgc ttgcaagaat atttgcaggg tcgccatctg
     7561 ccgctgccga cttatctggt agtccaggta cgtggcgaag cgcacgatca ggaatttact
     7621 atccactgcc aggtcagcgg cctgagtgaa ccggtggttg gcacaggttc aagccgtcgt
     7681 aaggctgagc aggctgccgc cgaacaggcg ttgaaaaaac tggagctgga atgagcatcg
     7741 ataaaagtta ctgcggattt attgccatcg tcggacgtcc gaacgttggc aaatccacat
     7801 tgttgaacaa actgctgggg cagaagatct ccatcacctc ccgcaaagcg caaacaactc
     7861 gtcaccgcat tgtggggatc catactgaag gcgcgtatca ggcgatctac gttgataccc
     7921 ctggcctgca tatggaagaa aaacgcgcta ttaaccgcct gatgaacaaa gcggcgagca
     7981 gctctattgg cgacgttgag ctggtgattt ttgtcgttga aggcacccgc tggacgccgg
     8041 acgacgaaat ggtgctcaac aaactgcgcg atggcaaagc gccggtgatc ctcgcggtga
     8101 acaaagtgga taacgtgcag gaaaaagccg atctgctgcc gcacttacag ttcctggcaa
     8161 gtcagatgaa cttcctcgat atcgtgccga tctctgctga aaccgggctg aatgtcgaca
     8221 ctatcgcagc aatcgtgcgt aagcatctgc ctgaagcgac ccatcacttc ccggaagatt
     8281 acatcaccga tcgctcacag cgttttatgg cgtctgaaat catccgcgaa aaactgatgc
     8341 gtttcctcgg cgctgaactg ccgtactccg tgaccgtgga gatcgaacgt ttcgtctcta
     8401 acgaacgcgg tggttatgac atcaatggtt tgattctcgt tgagcgtgaa gggcagaaga
     8461 agatggttat tggcaacaaa ggggccaaga tcaaaaccat cgggattgaa gcgcgtaaag
     8521 acatgcagga aatgttcgaa gcgcctgtcc accttgaact gtgggtaaaa gtgaaatccg
     8581 gttgggccga cgacgaacgc gcactgcgca gtctcggtta cgttgacgat ctttaagagt
     8641 cactccgatg gaaggctggc agcgcgcatt tgtcctgcat agtcgcccgt ggagcgaaac
     8701 cagcctgatg ctggacgtct tcacggagga atcgggtcgc gtgcgtctgg ttgccaaagg
     8761 cgcacgctct aaacgctcta ctctgaaagg tgcattacag cctttcaccc ctctcttgct
     8821 acgtttcggc gggcgtggcg aagtcaaaac gctgcgcagt gctgaagctg tctcgctggc
     8881 gctgccatta agcggtatca cgctttacag cggtctgtac atcaacgaac ttctctcccg
     8941 cgtactggaa tacgagacgc gcttctctga actctttttc gattacttgc actgcattca
     9001 gtctcttgca ggtgccactg gtacgccaga acccgcgctg cgccgctttg aactggcact
     9061 gctcgggcat ctgggctatg gcgtcaattt tacccattgc gcgggtagcg gcgagccggt
     9121 agatgacacc atgacgtatc gttatcgcga agaaaaaggg tttatcgcaa gcgtcgttat
     9181 cgacaataaa acgttcaccg gaaggcagtt aaaagcgtta aacgcgcggg aatttcctga
     9241 cgcagacaca ctacgcgccg cgaaacgctt tacccgcatg gcgcttaagc cgtatcttgg
     9301 cggtaaacca ttaaagagca gggaactgtt ccggcagttt atgcctaagc gaacggtgaa
     9361 aacacattat gaatgatgag gattgtcatg gctgaattac tgttaggcgt caacattgac
     9421 catattgcaa cgctgcgtaa cgcacgcggt actgcttacc cggatccggt gcaggcggcg
     9481 tttattgccg agcaggcggg agcggacggc attaccgtgc atttacgtga agatcgccgc
     9541 cacattactg accgcgatgt gcgcatcctg cggcagacgc tggatacccg aatgaatctg
     9601 gagatggcag tgaccgaaga gatgctggcg atcgccgttg agacgaagcc acatttttgc
     9661 tgcctggtac cggaaaagcg tcaggaagta acaaccgaag gcggcctgga cgttgcaggg
     9721 cagcgtgaaa aaatgcgcga tgcctgcaaa cgtctgacag atgccgggat tcagatttct
     9781 ctctttatcg acgccgatga agagcagatc aaagctgcgg cagaggttgg cgcaccgttt
     9841 atcgagatcc acaccggttg ctatgctgat gccaaaactg acgccgaaca ggcgcaagag
     9901 ctggtgcgta tcgccaaagc cgcgaccttt gccgctagtc tcggtctgaa agttaacgcc
     9961 ggacacggtc tgacctatca caacgtgaaa gccattgccg ccatccctga gatgcatgaa
    10021 ctgaatatcg gtcatgccat tattggccgt gcagtgatga ccggactgaa agatgcggtg
    10081 gcagaaatga agcgtctgat gctggaagcg cgtggctaat ggcaatatta ggtttaggca
    10141 cggatatcgt agagatcgct cgcatagaag cagtgatcgc ccgatccggt gagcgcctgg
    10201 cacgccgcgt attaagcgat aacgaatggg caatctggaa aacgcaccac cagccggtgc
    10261 gttttctggc gaagcgtttt gctgtgaaag aagccgcagc aaaagcgttt ggcaccggga
    10321 tccgcaatgg tctggcgttt aatcaatttg aagtattcaa tgatgagctc ggcaaaccac
    10381 ggctacggct atggggtgag gcattaaaac tggcggaaaa gctcggcgtt gtaaatatgc
    10441 atgtaacgct ggcagatgaa cgccactatg cttgtgccac ggtaattatt gaaagttaaa
    10501 ataataccgt tctctttaaa aagtaaaacc cccgaactgg taaggaacgg gggttttact
    10561 ttttaacttt aattttgtgc agcatgcacc ctcaacaaca gcgattatgg atcagtgtac
    10621 ttatctttta caagcaactt gcaatcttta gcataaaaac tcgagccttt acgaagaaag
    10681 caatactgaa cggctgcatt ccttgtttaa ggaaaaacga atgactgatt gccgatacct
    10741 gattaaacgg gtcatcaaaa tcatcattgc tgttttacag ctgatccttc tgttcttata
    10801 acacaaggaa acgtacttaa ggtgcgtccg gtgaaccaat cggacgcgcc tttaataact
    10861 ataaataagt gtcttggcag atactatata aattaactta gtgaatgatt atgctaatgt
    10921 catcaattaa ataaatataa tggcgttaag gcttcccagt aatataatta atactctact
    10981 tccagagtag aatattaaat tttatccgcg tggtgcatca gcacaaattt atcccacaac
    11041 tgttcttctg tctcgacatg cgccggatct ttcacaatag tattggggat cgggcacacc
    11101 ttctggcagg ttggtgtctc gtagtgccct acgcattcgg tacacttatc gctgttaatc
    11161 tcgtagatat gatctcccat tgaaatcgcc tcattcgggc attcgggttc acacatatca
    11221 caattaatac agcgtttagt aattagtaaa gacatttcaa tggattaccg ttaaatcatt
    11281 ttaaaatcag taagttgtat cgagtttgta tgctttactg tcattaactt actgtatgtt
    11341 gatccagtgt atttaacctt gataaactca gtccagcaac acaaaaccgc aacacattgc
    11401 attttgtccc gtagaaaaga cttgtatgtg tgagcttgtt ttctgcgcct acgcagataa
    11461 ggattgagaa tgccgcgcac tgtaacacat aatccggata gccccaataa tgacgatgtt
    11521 ttagccgcat ctgaaaaatg ggacgcctgt aaacccccct ataccagcgc acacatgaaa
    11581 atctgtgttg ctgccgccaa aatcatcctc gctgcttccg gcgtggctcg ccgttccaaa
    11641 tacgaaaaag agaactatct ccgtatcgat ttcagcaaag ccggtaaggt tacattttac
    11701 gccgagtttc caaaaaagat gggcctcaaa ggtaaaaagc tcggcgagtg gccggagctc
    11761 gctatccagc tggcgcgcga aaaagcacta ggtatggctg acggtggact gcgggcagag
    11821 tccgtacatg cagcgctgga aatgtaccgg gatgacctca aagccaaagt agcccggcag
    11881 aagctgagcc cggacagttt cacaacctac ggggtgcgta tcgaccggat taaagcaacg
    11941 ttcggcgagc gtgaggtgtt cagcgacgta acatacaatc ggctggtgga agtgctggac
    12001 gagtggatcg ccactcgctc gaacaataac gccctggagt tgtttgccga gctccgtcgg
    12061 ttctggaagt tctgcgcacc tactctttgc aacggccgca atgttgccgc cagtctgcca
    12121 gatgattatg tttcctcccg cgtacagaaa cctaccccca cacggctttt taccgatatt
    12181 gaatcaatcg cccgactctg gctcaatgtt gctgcctgca cctctgtaca ccagaagaat
    12241 gctgttcgct tcatgatcat cactggtgtt cgtccgatta atgtccataa cctgcgctgg
    12301 gactacgttt acgaggaggc tggtgaaatt gtttatccgg aaggggttat cggcatgcga
    12361 ggggctatga aaacacaaaa ggctttccgc ctgccgataa cgcctgagat ccggcggatt
    12421 atcgacgagc agaaagcctg gcgtgattca gttcctgagt gcaacaggga ttatgtattt
    12481 ttgcagccac gtgatccaat gcagccattt tcaaaacgat cactggataa gctggtgaaa
    12541 acatacagcc cggacggggc tgtaaaagga ataaaacatg atgggactgt taaagggaaa
    12601 gacggtgcat ttaatacgat gtgccgtaaa ttccttaaga gcaatgttat tgccttgatc
    12661 gataggaatt aaaaccccaa aaagattaaa aaaacaccac aaaacggatg tttcttcaac
    12721 accacttttg ctcatatgaa cggaaccgac gattaaactg gatggctctg attgattcag
    12781 ggtatgaatg gcggtttttt gctccgtttc cctcaaaatg gacgcaactt tccctctgcg
    12841 gctctcagcc gcaccaccgc atccgggcca gcggctcatg catcaggacc tgctctgcca
    12901 gacggtagcc ccgcttcagc cccgtaaaac gcatctgact cccgcacagc acgcacttca
    12961 gcgggtcaac cttcagtaac ctctgataca tccctctcca ggtgatttgc atcgccgttt
    13021 ttctcactgt ctccgttatg atgtacacca cttcttccag taaccgccgt ttcgccggac
    13081 tcaaaaaacc gtagtacctc accatacgga accccttatc cgccacatgc caggagaacc
    13141 tttccatgaa ctcatctcca ctcatcaaca ggtattcttc ccgttttgtt cggtgactgt
    13201 tgtaacgcag accgatttca tcctgaccgg cataatgctc cagacgactc atcggcaccg
    13261 gtggcttttt caggtaagag ccaaagtaca ccgccacatg gggggcatta tccatcaccc
    13321 gggatacgtt gacattccag ccacggcggt aatgcgtgtc caggaagcga ttccattccc
    13381 gtttactgct tccttctgct gccagcgcat ccggcatcac caggtcaggg tatttccgtg
    13441 acagcaaccg tgttatccgg tagcgccaca tgctcatcac cttacgggcg taaaaatgaa
    13501 gatttttcca ggtgtggccc gacgtcacac caccggcagt tgtcgataaa tggatatgcg
    13561 gatgccactg ctggtcacgc ccccatgtgt ggatcaccgt gaatatcccc ggctccacat
    13621 ctgcctgatg gcagatttcc agtatcacat ccgctgcaat gcggctcatc tctgtcagta
    13681 accaccggtt gtggaacacc agggaccagt actggcaggg aagtgtgaac acaatatgct
    13741 gccacgggca gtcggggacc aggctcagca gatactgtat ccactgtgcg ccagccttca
    13801 ccccgcagtg cgggcaggag cggcttttac accggaagca gacctttttt gtatggcaac
    13861 agtccggtga tgaacagcac cactgtgtat accccatcag tgtggtcccg cacgccatga
    13921 ttttggtcac cgactcaatc accaccggac gtactgccct ttccggctgc ttctccagcc
    13981 agttaagcca gtggtttccc tgctgaaaga tatcggcaaa acggggaagc atcagaaggg
    14041 cggggcgact ccgtccggcc agtgaaccgt gccacactcc gggcagtaca taccgccggc
    14101 gctgataccg gaaagaatgg tcgcaaattc ccgctccgtg cagcgggcga tttccggata
    14161 cccttcgtca tcaacacgta caaaccagaa gaccagcttt ttgtttcccg catccacaaa
    14221 gaacggaata ttcaggtctg cgcagcattc aacggcatcg tcaaaactat caaagcgcag
    14281 aacttctgcg tcttcttcgt caaaaaaatc atcttcgtga agcttcacga catagcgggg
    14341 aagtttgctt ctttgagagg cgggtttac
//
LOCUS       DAJTZL010000103        14306 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.103, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000103 DAJTZL010000000
VERSION     DAJTZL010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14306
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.103"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            19..1245
                     /gene="rtcB"
                     /locus_tag="OO544_002684"
     CDS             19..1245
                     /gene="rtcB"
                     /locus_tag="OO544_002684"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HCR6768247.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPFNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESEQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRD
                     LAYFMEGTEYFDDYLRAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQHFGEEIYVTRKGAVFARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            1249..2265
                     /gene="rtcA"
                     /locus_tag="OO544_002685"
     CDS             1249..2265
                     /gene="rtcA"
                     /locus_tag="OO544_002685"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HCR6768248.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITSIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRLGTVRGGEYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFNTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGSFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAVVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(2308..5013)
                     /gene="malT"
                     /locus_tag="OO544_002686"
     CDS             complement(2308..5013)
                     /gene="malT"
                     /locus_tag="OO544_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HCR6768249.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSWICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKTFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIHTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            5624..8017
                     /gene="malP"
                     /locus_tag="OO544_002687"
     CDS             5624..8017
                     /gene="malP"
                     /locus_tag="OO544_002687"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HCR6768250.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            8027..10111
                     /gene="malQ"
                     /locus_tag="OO544_002688"
     CDS             8027..10111
                     /gene="malQ"
                     /locus_tag="OO544_002688"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HCR6768251.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVKA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVADQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRNDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(10156..11472)
                     /gene="gntT"
                     /locus_tag="OO544_002689"
     CDS             complement(10156..11472)
                     /gene="gntT"
                     /locus_tag="OO544_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HCR6768252.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIDSIKVGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKRIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(11833..12408)
                     /gene="nfuA"
                     /locus_tag="OO544_002690"
     CDS             complement(11833..12408)
                     /gene="nfuA"
                     /locus_tag="OO544_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HCR6768253.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            complement(12467..13150)
                     /gene="gntX"
                     /locus_tag="OO544_002691"
     CDS             complement(12467..13150)
                     /gene="gntX"
                     /locus_tag="OO544_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HCR6768254.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSLLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            13188..13958
                     /gene="bioH"
                     /locus_tag="OO544_002692"
     CDS             13188..13958
                     /gene="bioH"
                     /locus_tag="OO544_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HCR6768255.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSQGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDDWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVPMPFLRLYGYLDGLVPRKVVPML
                     DKLWPRSKSYIFAKAAHAPFISHPVEFCHLLVALKQRV"
     gene            complement(13987..>14306)
                     /locus_tag="OO544_002693"
     CDS             complement(13987..>14306)
                     /locus_tag="OO544_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HCR6768256.1"
                     /translation="LMGLVEQMACLLSSGYANDRQIKGLFNYILQTGDAVRFNDFIDG
                     VAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLADIMRFTGVSEEELAA
                     ASQ"
BASE COUNT         3442 a         3568 c         3996 g         3300 t
ORIGIN      
        1 cgaaaagaga aaaacaaaat gaattacgaa ttactgacca ctgaaaatgc cccggtaaaa
       61 atgtggacca aaggcgtgcc ggtagaagcc gatgcgcgtc agcaacttat taatacggcg
      121 aagatgccgt ttattttcaa acatattgcg gtaatgcctg atgtacacct gggtaaaggt
      181 tccaccattg gtagcgtgat cccgaccaaa ggggcgatta ttccggcggc ggtgggcgtg
      241 gatattggct gtggaatgaa cgcgctgcgt accgcgttaa cggcggcaga cctgcctgaa
      301 aacctggcgg agctgcgtca agcgattgaa acggccgtac cacacgggcg tactaccggg
      361 cgttgtaaac gtgataaagg cgcatgggaa aacccgcctt ttaacgtcga tgcgaaatgg
      421 gctgagctgg aggccggtta tcagtggtta acgcaaaaat atccgcgttt ccttaacact
      481 aacaactata aacacctggg aacgctggga acgggtaacc actttattga aatctgcctt
      541 gatgagtcgg agcaggtgtg gattatgctg cactccggtt cacgcggaat tggtaacgcc
      601 atcgggactt actttatcga tctggcgcaa aaagagatgc aggaccaact tgagacgttg
      661 ccgtcgcgtg atctggcgta ctttatggaa ggtacggaat actttgatga ttacctaaga
      721 gccgtggcct gggcgcagct tttcgccagc cttaaccgcg atgcgatgat ggaaaacgtg
      781 gtaacggcat tgcagagcat tacgcagaaa acggtcagac agccacaaac gctggcgatg
      841 gaagagatca actgtcacca caactatgtg caaaaagaac agcactttgg tgaagagatc
      901 tacgtgacgc gtaaaggcgc ggtgtttgcg cgtgctggtc aatatggaat tattccaggg
      961 tcgatgggag caaaaagctt tatcgtccgt gggctgggaa atgaagaatc tttctgttcg
     1021 tgcagccacg gtgccgggcg ggtaatgagc cgtactaaag cgaaaaaact gttcagcgtg
     1081 gaagatcaaa ttcgtgccac cgcgcatgtg gaatgccgta aagatgccga agtgatcgac
     1141 gaaatcccga tggcgtataa agatattgat gcggtgatgg cggcacaaag cgatctggtg
     1201 gaagttatct ataccctgcg tcaggtggtg tgcgtaaaag gataaatgat gaaaaggatg
     1261 attgcgctgg atggcgcaca gggcgaaggt ggcgggcaga tcctgcgctc ggcgctgagc
     1321 ctgtcgatga taaccggcca gccatttacc atcaccagca ttcgtgccgg gcgggcgaaa
     1381 ccggggctgt tgcgccagca tctgaccgcg gtaaaagcgg ctgcggaaat ttgtagggca
     1441 acggtggaag gcgcggagct ggggtcgcag cgtctggtct tccggcttgg caccgtgcgc
     1501 ggcggcgagt accgctttgc tatcggtagt gccggaagct gtacgctggt gctgcaaacg
     1561 gtgctgcccg cgctgtggtt tgccgatgga ccttcgcgtg ttgaagtgag cggaggcacc
     1621 gataacccgt cggccccgcc tgcggatttt atccgccggg tgctggagcc gctgctggcg
     1681 aaaataggaa ttcatcagca aaccacgctg ttacgtcacg gtttttatcc tgccggaggc
     1741 ggcgtggtgg caacggaagt ctcgcccgtg gcatcgttta acaccttgca acttggcgag
     1801 cgcgggaaca ttgtgcagat gcgtggagaa gttctattag ctggtgtgcc gcgccatgtt
     1861 gctgagcgtg aaatcgctac actggcgggg agtttttccc tgcatgaaca gaatattcat
     1921 aacctgccgc gcgaccaggg gccgggtaat accgtctcgc tggaagtcga aagtgaaaat
     1981 atcactgaac gctttttcgt cgtcggtgaa aagcgcgtca gtgccgaggt ggtagccgcg
     2041 cagttagtga aagaggtgaa acgctacctg gcaagcccgg cggtagtggg ggaatatctc
     2101 gccgaccagc tggtgctacc gatggcgctg gcgggcgcgg gagaatttac ggtcgcccat
     2161 ccttcatgcc atctgctgac caatatcgcc gtggtggagc gtttcttgcc ggtgcggttt
     2221 ggtttgatag aaacagatgg cgtaacgcgg gtgagtattg aatgatgtaa agatgtaaac
     2281 cggatccaat gcattatccg gctaaactca caccccatat cccatcatct tcagcagttt
     2341 ctgcgcgtgt tgtaccgcgg cctgacggtg ggcgacgccg agtttttggt acagattgcg
     2401 gatatgcgtc ttgatggtgg ttgccgccac ttccagctcg cccgcaattt gctcattgct
     2461 gtaaccggag tagatcagcc ccaggacctg ccattcacgt tgggtcaacg ggctggtgtg
     2521 gatcagttct ggcacttccg gatgattcag cagacgttca acgaaattct catcgaaatg
     2581 ggcgaattta tgccgatgat gttgattgat ttctcgcaga atacgctgcg cgcgatgctg
     2641 ttccagttcc ggcagcgtat tgagttgaat cagctggcgc agttgttgcg ccatcgcttc
     2701 gccttcgatg acaaaatgac tgataaatcc ggtgcgattc gccagtttta atgcgtccag
     2761 caacacgcgc tgggcgtcac ttttacgtcc ggcctgccag tacagttgat taagcagcaa
     2821 caggttacgg ttgagatcgc tcatcaaccg cagactgcgg gcattttcat tgagttcttc
     2881 gagaacaatt tccgcaggtt caaactcgcc cagcaagatt tgtgcgcggg caatgttgcg
     2941 ccattgacct tgcaggaagt ggttgttcgc aaactccggt ttagccgtat gacgcaacca
     3001 gttggcagcg gcggctttat cgccggtcat ttgccagtaa atcacccgga ctttgttggc
     3061 gttagagatc cagtcgctgt gatatttgcc attccccagc aggttttcca gacggttcag
     3121 ctggctacgg gcgttatcta aatcaccacg ggccagcgag cattgaatca acattgccag
     3181 gcactgaagc tgttgctgtg gctgataaga cgacaagact tcaatcccgc tacgcgccga
     3241 cgcttcggct tcatccagcc gtgcccaggc ccataacagt tgtgctcgga tgcgcaccag
     3301 aaactcatgc atcggcagct gttccagatg ctgctcgttg atcagctgga acgttttttc
     3361 ctgtgtttcc cacgcggttt gcaggaaccc ttgggcaaac agaatttcac tttgctggat
     3421 taaactccat aaggcgtagt gccagacgtc gtgctggcgt gccatctgtt cggtttgctg
     3481 cattagcgcc agtgagcggg tcaattcgcc tttgcagtgc agcacttcac ccagcaccga
     3541 ggttgctaca atgcggctat agaaccagcc cggtggcagc tcttccagtg ccagttttgc
     3601 cagccgttcc gcttcatccg gattaccatc gttaatcgcc acctgggcgc gcagagcgtt
     3661 aaattctgcg tgcatggtgc cttctctgat gtccttgatt tcatgttcag cacgggctag
     3721 cagggtgtta acttcgccat agcgatgttg gctttgcatt agccacgcct gcaataacac
     3781 taactgcgga ttttccagca aactgtccca cggcagggcc ttaagcgact cttccagcag
     3841 cgacagttcg ctatggttga acaaactcca ggcgtgatta agcagaatat cgcgcagcat
     3901 cagcgcatcg cctgccgcca gcgcatgatg aatcgcttcg ctgggaaatc cctgggccat
     3961 ccagctttct gcggcggcac ggtggatttc cggcagctcc gccgccagtt cccactggca
     4021 gcgctggcgc aggaagttgc caaacagcgg gtgatagcag aaccactcgc cggtatcatc
     4081 catccgctgt aaaaacagcc cctgacgctc aatctcttcg agacgcattt gcccgttttc
     4141 ttcgccagtc acacgggtga tgagggcatc gttcattgag cgcaaaatgg cgcttttcaa
     4201 cagaaaatgg cgcgttgcga gatcgacgtt atccaaaacc tcatcgacca gataatccga
     4261 aagatggctg gcattgattc ccgccaggcg gcgtgccgac ttatgggctg agtgggtatt
     4321 ctgccgggcg gagagggcga ttagctgtag tgccgtcgcc caaccggaaa cgtcatcgca
     4381 aatccaactg ctttctgcgg cttcaatcgg cgatgacaga cggcaatcaa aaaactgctt
     4441 cgcttcctga tgggtaaatg ccagttgctg actgccaatt tccagcagtt ggtcacgaac
     4501 acgcagattg gcaatgccca gttgcggaag gttgcgtgac aacaccacca gggtgagatt
     4561 ttctggttga tggcgaataa agaagcgcat tgactcgtgg atcactggat tagtgatcag
     4621 atgatagtca tcgatgacca gataaagtgg gctatgccat tccgccagct caatgaaaag
     4681 ctgggcgaag agtgacgtca ggctggcata ttgccgtttt tgcgccatcg tctcacatat
     4741 cgcacagtga ccgttggttg cctgctgcac ggcggcaatg agatagctgg cgaaacgctc
     4801 ttgctggtta tcaccttcat ccagcgagta ccagccgata tcgtttttgc ctgccgccca
     4861 ctgggaaatg agggtggtct ttccgtagcc cgcaggactc gtgatcagcg ccagccggaa
     4921 gttgttcgcg ccggaaagtt tagccagcag gcgctcacga accacggtat ggtcgagtcg
     4981 aaccggacga cttagttttg acggaatcag catagttaat cacttcactg tggaaaatga
     5041 ggaaatatta tttttttgcg cttcgtaatt aatggttata aggtcggcca gaaacctttc
     5101 taatgcaagc gatgacgttt ttttatgtgt ctgaatttgc actgtgtcac aattccaaat
     5161 ctttattaac aactcaccta aaacgacgct gatccagcgt gaatactggt ttcccttatg
     5221 ttcatcagat tcatttaagc aagggtttct tcttcattcc tgatgaaagt gccatctaaa
     5281 aagatgatct taataaatct attaagaatg agatggagca cattggatat tttacttgtg
     5341 aaactgtttc actcctttac ttaatttata gagttacctt ccgctttttg aaaatacgca
     5401 acggccattt tttgcactta gatacagatt ttctgcgctg tattgcattg atttgctgct
     5461 aatcctgtgg tttgcactag ctttaagtgg ttgagatcac atttccttgc tcatccccgc
     5521 aactcctccc tgcctaatcc cccgcaggat gaggaaggtc aacatcgagc ctggcaaact
     5581 agcgataacg ttgtgttgaa aatctaagaa aagtggaact cctatgtcac aacctatttt
     5641 taacgataag caatttcagg aagcgctttc acgtcagtgg cagcgttatg gcttaaattc
     5701 tgcggctgaa atgactcctc gccagtggtg gctagcagtg agtgaagcac tggccgaaat
     5761 gctgcgtgct cagccattcg ccaagccggt ggcgaatcag cgacatgtta actacatctc
     5821 aatggagttt ttgattggtc gcctgacggg caacaacctg ttgaatctcg gctggtatca
     5881 ggatgtacag gattcgttga aggcttatga catcaatctg acggacctgc tggaagaaga
     5941 gatcgacccg gcgctgggta acggtggtct gggacgtctg gcggcgtgct tcctcgactc
     6001 aatggcaact gtcggtcagt ctgcgacggg ttacggtctg aactatcaat atggtttgtt
     6061 ccgccagtct tttgtcgatg gcaaacaggt tgaagcgccg gatgactggc atcgcagtaa
     6121 ctacccgtgg ttccgccaca acgaagcact ggatgtgcag gtagggattg gcggtaaagt
     6181 gacgaaagac ggacgctggg agccggagtt taccattacc ggtcaagcgt gggatctccc
     6241 cgttgtcggc tatcgtaatg gcgtggcgca gccgctgcgt ctgtggcagg cgacgcacgc
     6301 gcatccgttt gatctgacta aatttaacga cggtgatttc ctgcgtgccg aacagcaggg
     6361 catcaatgcg gaaaaactga ccaaagttct ctatccaaac gacaaccata ctgccggtaa
     6421 aaagctgcgc ttgatgcagc agtacttcca gtgtgcctgt tcggtagcgg atattttgcg
     6481 tcgccatcat ctggcggggc gtaaactgca cgaactggcg gattacgaag ttattcagct
     6541 gaacgatacc cacccaacta tcgcgattcc agaactgctg cgcgtgctga tcgatgagca
     6601 ccagatgagc tgggatgacg cttgggccat taccagcaaa actttcgctt acaccaacca
     6661 taccctgatg ccagaagcgc tggaacgctg ggatgtgaaa ctggtgaaag gcttactgcc
     6721 gcgccatatg cagattatta acgaaattaa tactcgcttt aaaacgctgg tggagaaaac
     6781 ctggccgggc gatgaaaaag tgtgggccaa actggcggtg gtgcacgata aacaagtgca
     6841 tatggcgaac ctgtgtgtgg ttggcggttt cgcggtgaac ggtgttgcgg cgctgcactc
     6901 ggatctggtg gtgaaagatc tgttcccgga atatcaccag ctatggccga acaaattcca
     6961 taacgtcacc aacggtatta ccccacgtcg ctggatcaaa cagtgcaacc ctgcactggc
     7021 ggctctgttg gataaatcac tgcaaaaaga gtgggctaac gatctcgatc agctgatcaa
     7081 tctggaaaaa ttcgctgatg atgcgaaatt ccgtcagcaa tatcgcgaga tcaagcaggc
     7141 gaataaagtc cgtctggcgg agtttgtgaa agttcgtacc ggtattgaga tcaatccaca
     7201 ggcgattttc gatattcaga tcaaacgtct gcatgagtac aaacgccagc acctgaatct
     7261 gctgcatatt ctggcgttgt acaaagaaat tcgtgaaaac ccgcaggctg atcgcgtacc
     7321 gcgcgtcttc ctcttcggcg cgaaagcggc accgggctac tacctggcga agaatattat
     7381 ctttgcgatc aacaaagtgg ctgacgtgat caacaacgat ccactggttg gcgataagtt
     7441 gaaggtggtg ttcctgccgg attattgcgt ttcggcggcg gaaaaactga tcccggcggc
     7501 ggatatctcc gaacaaattt cgactgcagg taaagaagct tccggtaccg gcaatatgaa
     7561 actggcgctc aatggtgcgc ttactgtcgg tacgctggat ggtgcgaacg ttgaaatcgc
     7621 cgagaaagtc ggtgaagaaa atatctttat ttttggtcat accgtggaac aagtgaaggc
     7681 aattctggcc aaaggctacg acccggtgaa atggcggaag aaagataagg tgctggacgc
     7741 agtattgaaa gagctggaaa gcggtaaata cagcgacggc gataagcatg ccttcgacca
     7801 gatgctgcac agtatcggca aacaaggcgg cgatccgtat ctggtgatgg cggatttcgc
     7861 agcctatgtt gaggcacaaa agcaggtgga tgtgctgtac cgcgaccagg aggcctggac
     7921 tcgcgcggcg atcctcaata ccgcccgctg cggtatgttt agctcggatc gctctattcg
     7981 cgattatcag gctcgtatct ggcaggcaaa acgctaagga agctcgatgg aaagcaaacg
     8041 tctggataat gccgcgctgg cggcggggat tagccccaat tacatcaatg cccacggtaa
     8101 accgcagtcg attagcgccg aaaccaaacg gcgtttgctt gacgcgatgc atcaacgtac
     8161 cgccacgaaa gtggcggtaa cgccagtccc gaatgtcatg gtttatacca gcggcaaaaa
     8221 aatgccgatg gtggtggagg gcagcggcga atatagctgg ctgctgacca ccgaagaagg
     8281 aacacagtac aaaggccatg taacgggggg caaagcgttc aatctaccga cgaagctgcc
     8341 ggaaggttat cacacgctga cactcaccca ggacgaccag cgcgcgcatt gccgggtgat
     8401 tgtcgccccg aaacgctgtt acgaaccgca ggcgttgctg aataaacaaa agctgtgggg
     8461 tgcctgcgtt cagctttata cgctgcgatc ggaaaaaaac tggggtattg gggattttgg
     8521 cgatctcaaa gcgatgctgg tggatgtggc aaaacgtggc gggtcgttca tcggcctgaa
     8581 cccgattcat gcgctctatc cggcaaatcc ggagagtgcc agcccataca gcccgtcttc
     8641 tcgccgttgg ctgaatgtga tttatatcga cgttaaagcc gtcgaagatt tccatcttag
     8701 cgaagaggct caggcctggt ggcagttgcc gaccacgcaa cagacgctgc aacaagcgcg
     8761 ggatgccgac tgggtcgatt actccacggt taccgcccta aaaatgacag cattacgaat
     8821 ggcgtggaaa ggtttcgcgc aacgtgatga tgagcagatg gccgcgtttc gccagtttgt
     8881 tgcagatcaa ggcgacagcc tgttctggca ggcagccttt gatgcgctac atgcccagca
     8941 agtgaaagag gacgaaatgc gctggggctg gcctgcatgg ccagagatgt atcagaacgt
     9001 ggattcacca gaagtgcgtc agttctgcga agaacatcgt aatgacgtcg atttttatct
     9061 ctggttgcag tggctggctt acagccagtt tgccgcctgc tgggagataa gccagggcta
     9121 tgaaatgccg attggcttgt atcgtgatct ggcggttggc gtagcggaag gtggggcgga
     9181 aacctggtgt gaccgtgaac tatattgcct gaaagcgtcg gttggcgcgc cgccggatat
     9241 cctcggcccg ttggggcaga actggggatt accgccaatg gacccgcata tcatcaccgc
     9301 gcgtgcctat gaaccgttta tcgagctgtt gcgtgccaat atgcaaaact gcggcgcatt
     9361 acgaattgac catgtgatgt cgatgctgcg tttgtggtgg ataccgtatg gcgagacggc
     9421 agatcagggc gcgtatgttc actatccggt ggatgatctg ctctcgattc tggcactcga
     9481 aagtaaacgt catcgctgta tggtgattgg tgaagatctc ggtaccgtac cggtagagat
     9541 tgtcggtaag ctgcgcagca gcggtgtgta ctcctacaaa gtgctctatt tcgaaaacga
     9601 ccacgagaag acgttccgtg caccgaaagc gtatccggag cagtcgatgg cggttgcggc
     9661 gacacatgac ctgcctacgc tacgcggtta ctgggagagc ggggatctaa cgctgggcaa
     9721 aaccctgggg ctgtatccgg atgaagtggt actgcgcggt ctgtatcagg atcgcgaact
     9781 ggcgaagcaa gggctgctgg atgcactgca taaatatggt tgtctgccaa aacgtgccgg
     9841 gcataaggca tcgttgatgt cgatgacgcc gacgctgaac cgtggtttgc agcgctacat
     9901 tgccgacagt aacagtgctc tgttaggact acagccggaa gactggctgg atatggccga
     9961 accggtgaat attcctggca ccagttacca gtataaaaac tggcgacgca agctttccgc
    10021 aacgcttgag tcgatgtttg ccgatgatgg cgtgaacaag ttgctgaagg atttggacag
    10081 acggcgcaga gctgcagcga agaagaagta gagtgcggtt gatgccggat gcgatgcatc
    10141 cggcagtcag tgttatcaaa tcaccatatt cagcagcaga cagcctacca gaccgcacac
    10201 cgagataatg gtttccagca tcgaccagga tttgatggtc tcgccgatag tcaggttaaa
    10261 gtactctttg aacagccaga agcccggatc gttcacatga gagaaaatca cactaccgga
    10321 acccaccgca ataaccatca gctctgggct aacacccgtc gttgcaatca gcggtgccgc
    10381 gataccaccc gcagtgattg ccgcaacggt tgcggaaccc agcgcgatac gcagtacggc
    10441 agcaatcgac caggccatca gcagcggaga aatgttggtt tcgtgcatca tggaagcaat
    10501 gtatttgtcc acgccgctgt ctaccagcac ctgcttgaac gcaccgccac caccgatgat
    10561 caacagcatc atcgcaatga ttttgatgga agaaaccagc gtgtcgttaa tctgatccat
    10621 tgaacgacca cggttcagac caaaggtgaa catcgcaatc agcacggcaa tcagcgttgc
    10681 cattaccggg tcaccgagga actccgctac cggcaggaaa gcgtgacctt tcggcaggat
    10741 catttcggca atcgcacgca tcgccatcag cacaaccggc accagagaag tccagacgct
    10801 gacgccaaag ctcggcatct cttcttcgct gaaggttttc gcgctgtaga gaccttccgg
    10861 gattggctta tcgatacctt tcaacacgcg agcgtaaacc ggaccggcga gaatcacggt
    10921 cgggattgcc agaatagtac cgtacagcag ggttttaccc atatcggcat tgaaaatggt
    10981 ggcaatcgca gtcgggcccg gatgcggtgg caggaagccg tgggtcacag acagtgcagc
    11041 cgccattggt acaccaacat acagcagtgg aatattcgcg gaagccgcga tggtaaacac
    11101 cagcggcagc atcagcacga agcccacttc atagaacagg gcaaaaccaa cggtaaaacc
    11161 ggttaacacc accgcccact ggatgcgctt tttaccaaat ttggcaatca gcgtggtggc
    11221 gatacgttgt gcgccaccgc agtctgccag cattttgccc agcattgcgc caaaacccat
    11281 gatcagggca aggctaccga gcgtcccgcc gacaccgact ttgatggagt caataacttt
    11341 atccagcggc attccttgca ttaatccaac agcaagcgcc acgaggacga gagcgatgaa
    11401 gccgttcatt ttgaagcgga tcatcaggag caacaacaag attacaccga tagcaacaat
    11461 gactaatggc atgatttacc tggcctttca tttgttatgg gtaacgtcaa ttttctgacg
    11521 acaaactcta attatcccaa tcgggaacag agatattgcg gcaccacgac tgatacccac
    11581 taaaactaat tattgtagtc agatgtcagg agtatgtttg gtacccatgt gaatgatacg
    11641 ggtaacattt ggcgtttgag aatcaccaga gcggggtaaa tttaaattat gagaggttgg
    11701 tcatattatc gcggggaaac gaagggaggg atttgccaaa gcaatgctgt gccaacgtct
    11761 ggcacatgtt caacgtaggc ccgaaatgac gctttagcgt cgcatcgggc aatctacaaa
    11821 agaggggata acttagtagt aggagtgttc gccgcgctgg tgttcggtga gatcgcgcac
    11881 acctttcagc tccgggaatt cgttcagcag ctgcttctcg atcccttctt tcagcgtcac
    11941 atcgaccatg gaacaaccgt tacagccgcc gccaaattgc agaatggcgt aaccgtcttc
    12001 ggtgatttcc atcagcgaaa cgcgaccacc gtgaccagca agctgtgggt tgatctgcga
    12061 ctgcagcata tactccacgc gctccatcag cggtgcatcg tctgccactt tacgcatttt
    12121 ggcgttcggg gctttcagcg ttaactgaga acccaactgg tcggtaacaa agtcgatctc
    12181 tgcatcttcc aggtatggtg cgcttaactc atcaacatac gcggtcagca ggtcaaattt
    12241 cagggcagtg tcggtggctt ccacggcatc cggcggacaa taagaaacac cacattcagc
    12301 gttaggcgtg ccagggttaa tcacaaatac gcggatttgt gtcccttctt cctgatttgc
    12361 cagcagtttg gcaaagtgcg cttgtgcagc atcggaaata cggatcatag taatggccta
    12421 atagttgact attttagttg gttataatac gcccatcatc gaggctctac aaggttcgac
    12481 aaaggcacca gacctggaca gtcgccgcac cattgcgtaa aagcaactgc gcaatctctg
    12541 cgacggtact tccggtggta acgacatcat ccacaatcac catatggcga ccttgcacgg
    12601 gcaattcaag acgaaaggca tttttcaggt tgcgcttgcg cagccgggca ctgagaaaat
    12661 gctgggtcgc agtggcccgt gtacgtgtga cggcttcgct atcccattgg cagtgcaacc
    12721 agcgtgataa cggctgacac agcaaatcgc tctgattaaa tccccgacgc cagtgacgcc
    12781 gctgccataa cggaacgctg atgatgcgat ccggcaattg caacccggtg gtgcgacgag
    12841 cgtgtaagac ttccaatagt aacagacgtg acagggcgct ggcgatttca ctgcgccggg
    12901 aaaatttaag ctggtggatg agcagactta acggcggcgc atagtcggca accgtgacca
    12961 gtctttgcca gggcggcggt ttttgcaggc agcgaccgca gggaagatgg gagtgtgtgg
    13021 cgggtaatcc acattgtggg cataacgttt tatctgtgcg ggtggcgcgt gaacagaccg
    13081 aacaaatccc ccaatgacct aacgccagtg gcattcggca tagccagcat aatcccggta
    13141 ctgttagcat atgttcatcc ttgtaagtca aaagagaaca atagcggatg aataacattt
    13201 ggtggcagac caaaggtcag gggaatgttc atcttgtgct gctgcacgga tggggactga
    13261 atgccgaagt gtggcgttgc attgacgagg aacttagctc gcattttacg ctgcaccttg
    13321 ttgatctgcc cggcttcggg cgcagccagg gatttggtgc gctgtcactt gctgatatgg
    13381 ccgaagccgt gctgcaacag gcacctgata aagccatttg gttaggctgg agtctgggcg
    13441 ggctggtggc aagccagatt gcgttaaccc atcccgagcg tgttcaggcg ctggtcaccg
    13501 tggcgtcgtc accttgtttt agtgctcgtg acgattggcc ggggataaaa ccggacgtgc
    13561 tggcgggatt tcagcagcaa ctcagtgatg attttcagcg tacagtggag cggttcctgg
    13621 cgttacaaac catggggact gaaacggcgc gccaggatgc gcgggcgttg aagaaaaccg
    13681 ttctggcgtt accgatgccg gaggttgacg tgcttaatgg cgggctggaa atcctgaaaa
    13741 cggttgatct ccgtcagccg ctgcaaaacg tgcccatgcc gtttttgcga ttgtatggct
    13801 atctcgatgg tctggtgccg cgcaaagtgg tgccgatgct ggataaactc tggcctcgca
    13861 gcaaatcata tatcttcgcc aaagcggccc atgcgccgtt tatttcgcat ccggtcgagt
    13921 tttgtcacct gctggtggcg ttgaagcaga gggtgtaggc aacttttgaa atggcgagac
    13981 agaaatttac tggctcgccg cagccaactc ttcttctgac accccggtaa agcgcatgat
    14041 gtccgcaaga gggactccgg attcaagcat tattttggct atatgcaggg ctttggattg
    14101 ttccccctcc tgccgcaatc tttccgcaat tgtcattaaa ctctccttgt gtttcggtga
    14161 acgttcggca acgccgtcga taaaatcgtt aaaacgtacc gcgtcgccgg tttgcagtat
    14221 gtaattaaac agccctttga tttgtctgtc attagcgtat ccactactta ataagcaggc
    14281 catttgctct accagcccca tcaggt
//
LOCUS       DAJTZL010000104        14288 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.104, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000104 DAJTZL010000000
VERSION     DAJTZL010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14288
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.104"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..304)
                     /locus_tag="OO544_002694"
     CDS             complement(<1..304)
                     /locus_tag="OO544_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6768257.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGL"
     gene            complement(318..977)
                     /gene="pgmB"
                     /locus_tag="OO544_002695"
     CDS             complement(318..977)
                     /gene="pgmB"
                     /locus_tag="OO544_002695"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HCR6768258.1"
                     /translation="MKLQEVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNSQERAQLAYRKNLLYVHSLRELTVNAVLPGIRSLL
                     ADLRAQQISVGLASVSLNAPTILDALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGITGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(974..3241)
                     /gene="ycjT"
                     /locus_tag="OO544_002696"
     CDS             complement(974..3241)
                     /gene="ycjT"
                     /locus_tag="OO544_002696"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HCR6768259.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDVVGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGTGYTIASRRFVSADQLPLIVLEITITPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCKVSGDVQQCYTAKERRLLQHTS
                     AQLHAGETVTLQKLVWIDWRDDRQAVLDEWGSASLRQLEMCAQQSYDQLLAVSTENWR
                     QWWQKRRITVNGGEVHDQQALDYALYHLRIMTPAHDERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMAYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMAKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSAASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGGSVRDGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDF
                     ILPFNGTAATHQEDE"
     gene            complement(3238..4293)
                     /locus_tag="OO544_002697"
     CDS             complement(3238..4293)
                     /locus_tag="OO544_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR6768260.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSR
                     LSQAQALAEKYGNASVWDDPQAMLLAVKPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPLAMTPEQAREMCDTARKLGKVLAYDFHHRFALDTQQLREQVTNGVLGEIYVTTARA
                     LRRCGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYVLGFPAVKSVNAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQSIMNVSFCGDKAGA
                     TLFPAHIYTDNNGELMTLMQRKMADDNRHLRSMEAFINHVQGKPVMIADAKQGYIIQQ
                     LVAALYQSAETGTRVEL"
     gene            complement(4303..5091)
                     /locus_tag="OO544_002698"
     CDS             complement(4303..5091)
                     /locus_tag="OO544_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HCR6768261.1"
                     /translation="MKIGTQNQAFFPENILEKFRYIKEMGFDGFEIDGKLLVNNLEEV
                     KAAIKETGLPVTTACGGYDGWIGDFIEERRLNGLKQIERILEALAEVGDKGIVVPAAW
                     GMFTFRLPPMTSPRSLDGDRKMVSDSLRVLEQVAARTGTVVYLEPLNRYQDHMINTLA
                     DARRYIVENDLKHVQIIGDFYHMNIEEDNLAQALHDNRDLLGHVHIADNHRYQPGSGT
                     LDFHALFEQLRADNYQGYVVYEGRIRAEDPAQAYRDSLAWLRTC"
     gene            complement(5109..6161)
                     /locus_tag="OO544_002699"
     CDS             complement(5109..6161)
                     /locus_tag="OO544_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HCR6768262.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKVRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPEGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSAFRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(6192..6795)
                     /locus_tag="OO544_002700"
                     /pseudo
     CDS             complement(6192..6795)
                     /locus_tag="OO544_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415828.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
     gene            complement(6782..7663)
                     /locus_tag="OO544_002701"
     CDS             complement(6782..7663)
                     /locus_tag="OO544_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR6768263.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(7684..8994)
                     /locus_tag="OO544_002702"
     CDS             complement(7684..8994)
                     /locus_tag="OO544_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6768264.1"
                     /translation="MIKSKIVLLSALVSCALISGCKEENKTHVSIEFMHSSVEQERQA
                     VISKLIARFEKENPGITVKQVPVEEDAYNTKVITLSRSGSLPEVIETSHDYAKVMDKE
                     QLIDRKTVATVISNVGEGAFYDGVLRIVRTEDGSAWTGVPVSAWIGGIWYRKDVLAKA
                     GLEEPKNWQQLLDVAQKLNDPANKKYGIALPTAESVLTEQSFSQFALSNQANVFNAEG
                     KITLDTPEMMQALTYYRDLAANTMPGSNDMPGSNDIMEVKDAFMNGTAPMAIYSTYIL
                     PAVIKEGDPKNVGFVVPTEKNSAVYGMLTSLTITAGQKAEETEAAEKFVTFMEQADNI
                     ADWVMMSPGAALPVNKAVVTTATWKDNDVIKALGELPNQLIGELPNIQVFGAVGDKNF
                     TRMGDVTGSGVVSSMVHNVTVGKADLPGTLQASQKKLDELIEQH"
     gene            complement(9029..10539)
                     /locus_tag="OO544_002703"
                     /pseudo
     CDS             complement(9029..10539)
                     /locus_tag="OO544_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
     gene            complement(10751..11065)
                     /gene="pspE"
                     /locus_tag="OO544_002704"
     CDS             complement(10751..11065)
                     /gene="pspE"
                     /locus_tag="OO544_002704"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HCR6768265.1"
                     /translation="MFKKGLLALALVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            complement(11140..11361)
                     /gene="pspD"
                     /locus_tag="OO544_002705"
     CDS             complement(11140..11361)
                     /gene="pspD"
                     /locus_tag="OO544_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HCR6768266.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(11370..11729)
                     /gene="pspC"
                     /locus_tag="OO544_002706"
     CDS             complement(11370..11729)
                     /gene="pspC"
                     /locus_tag="OO544_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HCR6768267.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(11729..11953)
                     /gene="pspB"
                     /locus_tag="OO544_002707"
     CDS             complement(11729..11953)
                     /gene="pspB"
                     /locus_tag="OO544_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HCR6768268.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRDELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(12007..12675)
                     /gene="pspA"
                     /locus_tag="OO544_002708"
     CDS             complement(12007..12675)
                     /gene="pspA"
                     /locus_tag="OO544_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HCR6768269.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVNDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            12842..13819
                     /gene="pspF"
                     /locus_tag="OO544_002709"
     CDS             12842..13819
                     /gene="pspF"
                     /locus_tag="OO544_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HCR6768270.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNAVERSVYRHGTSDYPLDDIIIDPFKRRPPEDAIAVSETTSLPTLP
                     LDLREFQMQQEKELLQLSLQKGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(13937..>14288)
                     /locus_tag="OO544_002710"
     CDS             complement(13937..>14288)
                     /locus_tag="OO544_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCR6768271.1"
                     /translation="AAHAVLNIINKESLCERANQLGQRLKNTLIDAKESVPAIAAVRG
                     LGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYGNVIRFLYPLTIPDAQ
                     FDAAMKILQDALSD"
BASE COUNT         3427 a         3840 c         3520 g         3501 t
ORIGIN      
        1 tcaggccaaa cgccatgttg tcgtaaaccg tcatatgcgg atacaacgcg tagttctgga
       61 acaccatcgc tatattgcgt gctttggctg gaacgtcatt cattcgtttg ccgtcgatca
      121 acagatcgcc gccgctgatc tcctcaagcc cggcaatcat gcgcagggtg gtcgacttac
      181 cgcagcctga cggaccgaca aacacgatga actctttatc ggcaatttcc aggttgaagt
      241 ccttcaccac atgcacctgg ttatcgtaga ttttttgaat atgttgtaac gaaagctgag
      301 ccatgttgat tcctttgcta tacgttttgc cagaaggccg ataaccgcga ccaggtcagg
      361 gagtccgttg aaggcaatag taattgcgcc ccggttatgc ccgcgccgat ccccaccgag
      421 cgcataccgc tggcgttaat ggcgtcaatg cccgcctgcg catcttcaat gccgatacat
      481 gcctgcggcg gcacgcccag cccggcacag gcagcgagaa agatttccgg atccggtttc
      541 gagtttttaa gttgggaagc atccgcgcag aaggtgaaaa actcacgcag ctccagtgca
      601 tctaaaatcg tcggcgcatt gagggagaca gaagccagcc ctaccgagat ctgttgtgcg
      661 cggagatctg ccagcaaaga gcgaatgccg ggtagaacag cgttgaccgt caactcgcgc
      721 aatgagtgga catagagcag atttttgcga tacgccagtt gcgccctctc ctgcgagtta
      781 aagtcgccct ctttgccccc gtgttgcaga atgcgccgca gagactcatc gcggctgatc
      841 ccttttaggg attcgttaaa ctgcgcatca atgctgatgc caatttcagc ggcaatctgc
      901 tgccacgcct ggaaatgcag atgcgcggta tcggtgatta caccatccag atcgaaaatt
      961 acctcttgca gtttcattca tcctcctgat gtgtggcagc ggtcccattg aagggcaaaa
     1021 taaaatcacc caaacagaaa acagattctt cagccacgga tataagctga ccgttcaaac
     1081 gcagtgaaac gggggcagaa gttcgaatcg caatacgctg cgcgtcaaga gtgacctgta
     1141 attcgcagcc ctgccagaac agagggaaag acaactgttg ccactgctca ggtaacgccg
     1201 gattgagatg caattcaccg tcacgcacgc tccccccggc aaaaccctga atcgccccca
     1261 gccagatagc gccagttgcg gcagcatgga taccatcatc acaactatgc ggatcagcac
     1321 caagatcgat ttcagtcccc tcgcgccaga actgataact ttgggtcagc aggccacagc
     1381 gtgcggcaac aatgccgtgg attgctttac ttaatgacga gtcgtgaata gtgcgcggtt
     1441 cataaaattg cagattggca agacacgatg ccgctgagaa ctgctccggc agcatgtaat
     1501 tgagcatcac cacatcagct tgtttgagga tttgcatctc gttcacttct gcgcgtgaat
     1561 aatccagcag aatggtttgc ttccccgccg ccgctttgta tttcgcaaga ttaatcgccg
     1621 gcttagccat aaacgaatca tcctgcggca aaacgccgtc gggctgaatt tctggcagcc
     1681 gcagttcttt aaggaacatt tcggcacgat gaataaacgc gtcgtcgcta cagccaaact
     1741 ggcgggcaat actcagcgcc tgctggacgt tgtaatacgc catatagctg gtgaaggcgt
     1801 tattattgac atgttcggta tattcgtcag gcccaataac atcatgaatt tccagacggt
     1861 cgttaaccct caccgcgcgg ctaatccaga actttgcagt ttccagaagt agcgccatgc
     1921 cttcatgcgc aatgaaactt tcatcccccg tggtctgcca gtattgaata accgcccagg
     1981 cgatatcggc caccagatga tgttccgcct gcgccgaggc cactttttgc cgcagcccgg
     2041 tgcgaatgtt aatggcggca aattccggcg tctcttcttc gccgctgcgc gcgctttccc
     2101 acggaaatag cgcgccctgc cagccgttgc gtcgcgcttt ctcctgcgcg cctggcaagt
     2161 tgtgccagcg ataacgcagt aaacttcggg caaccgtcgg atcgctaaac agatgaaacg
     2221 gtaacaaaaa tacttctgta tcccagaaaa cgtggccttt gtagccttcg ccggttaagc
     2281 cttttgccgc aatgctgctg cgctcgtcgt gggcaggcgt catgatgcgc agatgataaa
     2341 gcgcataatc taacgcttgc tgatcgtgca cttcgccgcc atttaccgtg atacgacgtt
     2401 tctgccacca ttgacgccag ttttctgttg atactgcaag aagttggtcg taactctgct
     2461 gcgcgcacat ttcaagctgg cgaagcgacg cgctgcccca ctcgtctaaa acggcttgcc
     2521 tgtcatcccg ccagtcgatc cacaccagtt tttgcaacgt cactgtctcg cctgcatgaa
     2581 gctgcgcact ggtatgttgc agtaaacggc gctctttggc ggtatagcat tgctgcacat
     2641 caccgctcac cttacagcaa cagctgatgg ccacatcact gcgtccatcc tgggtggtgt
     2701 agatcccctg catcaaatgc tgaccaaaca cccgcacctg ggtttcgtcg agatgctggc
     2761 gaccgtggtt ggtctgcgtg gcgtcaatgc ctgttgaaat cagcactgac gcgtcggcgt
     2821 ccagtggcgt aatagtgatt tccagcacaa tgagcggcag ttggtctgcc gaaacaaagc
     2881 gacggctggc gatggtgtaa cctgtgccgt tgctggtacg ccagacaacg ctacggcgta
     2941 attctccact ggcaaagtca agttcacgct gccaggcttc gcgggataac gagaaaacct
     3001 caccatttat ggcaatctcc atccccacga catcaggcag gttcaccagt tcgttgattt
     3061 caccttttcc cgcccgatga tacagccccg ccagatacat ccctcgcgtc tggcgcgtgt
     3121 aatcttcttc atggctggcg cgaagcccaa gatagccgtt cccctgcgcc atcagcgatg
     3181 catacttgtt cagggtatgc tggctgaaat ggggttctga taacgttact ggcctggtca
     3241 taattccaca cgcgtccctg tttctgcgga ttgatacaac gccgccacca gttgctggat
     3301 gatgtacccc tgcttggcgt cggctatcat cacgggcttg ccctgtacgt gattgataaa
     3361 ggcttccatg ctgcgcaaat ggcggttgtc gtctgccatt ttccgttgca tcagcgtcat
     3421 taattcaccg ttgttatcgg tgtagatatg tgctggaaac agcgtcgcac cagctttatc
     3481 accacaaaag ctgacgttca taatcgactg ttcgcggatg ttgagtgcaa atgacgtttc
     3541 cagccacaga atgccgccgt tatgaaattc aatggtgcca aacagcgaat cttcgacgct
     3601 gtaagttgcc ggatcccact caccaaattg accacagctc ttttgcgtgc cgatcttttg
     3661 aaagctatgc gcattcacgc ttttcaccgc cggaaagccc agcacataca tcgcagcatc
     3721 cagcatatga atgccgatgt cgatcagcgg gccaccaccc tgcagttctt tattggtaaa
     3781 gacaccccag ccgggaacgc cgcagcgacg cagggcgcgg gcggtggtaa cgtaaatctc
     3841 tcccaaaacg ccgttggtca cctgttcacg cagctgttgc gtatcgagcg caaaacgatg
     3901 gtgaaagtcg taggccagca ccttgcccag tttgcgcgcg gtatcgcaca tttcccgcgc
     3961 ctgttctggc gtcatggcga gcggtttttc gcacatcaca tggcagcccg cttccagtgc
     4021 catcagggta tgttcgtaat gaaaacggtt aggtgagcag acgctaacca catcaggttt
     4081 caccgccagc agcatggcct gcggatcgtc ccacacggat gcattcccgt atttttctgc
     4141 cagcgcctgc gcctgggaaa ggcggctgtc acagacagcc accagttcca gatcgttgcg
     4201 ggtgcagtag tacgaagcat gaaccttatc cgccacctgg cctgcgccta ttatcgcgac
     4261 ccgcagcgga gagcttgtca ttgcactttt cacaaagacc tcttagcagg tacgcaacca
     4321 ggccaacgaa tcacggtacg cctgggcagg atcttccgcc cggatacgcc cttcgtacac
     4381 tacatagccc tgatagttat ccgcgcgcag ctgttcaaac agcgcgtgga aatccagggt
     4441 gccgctgccc ggctggtagc gatggttatc cgcaatatgc acatgaccga gcaggtcgcg
     4501 gttgtcatgc agcgcctgcg ccaggttatc ttcttcgata ttcatgtgat agaaatcgcc
     4561 gataatctgt acatgtttaa gatcgttttc gacgatgtaa cggcgggcat cggcgagggt
     4621 gttgatcata tgatcctgat agcggtttaa cggttcgaga tacaccactg ttccggtacg
     4681 cgcggcgacc tgttccagta cgcgcaggga atcactcacc attttgcggt cgccgtccag
     4741 gctacgcggc gaggtcatcg gcggtaagcg gaaggtaaac atgccccacg ctgccggaac
     4801 gacgatacct ttatccccca cttctgccag cgcttcgaga atgcgttcga tctgctttaa
     4861 gccattaaga cgacgctctt cgataaagtc gccaatccaa ccgtcatagc caccgcaggc
     4921 ggtggtcacc ggtaaaccgg tttctttgat tgctgctttg acttcttcga gattgttgac
     4981 cagcagtttg ccgtcaatct caaaaccatc gaagcccatc tctttgatat aacgaaattt
     5041 ctccagaatg ttttccggaa agaatgcctg attctgtgtg cctattttca ttttttcatt
     5101 cctttgaatt aaaacgtaac gcccattttg atgctctgtt ccggatgctg atcgacatac
     5161 tgcatgtagc tttccgggct attggcaaag gtcactaccg ggtcgattaa atcttcgcaa
     5221 ttgagataac cgttcatcag cagttcccag caggtttctt caatacgctt acggctccag
     5281 cgcggataat ccgggttcgg ttcgctgcac gcgcgggaga aaacaatttt ggcgttattg
     5341 aaatgcgctt cgcgtccgag gttaaaacct tcagcaaatg gtttggcaaa cgcaacatag
     5401 gagatggtgc caccgtaggc cagaccgcgg aacgccgatt gcagcgcgtc ggcatagccg
     5461 ctggtttcga taatcacatc ggcaccctgc ttgccggtca gcgttttgat ctctttgcct
     5521 acgtcagtac caatggggtt aaggcagaaa tccgcgccgt ggcgacgggc aatatcacag
     5581 cgatgagcga taggatcgac gccaatcacg accgacgcgc cagcacgttt agccagttga
     5641 atggcaattt gaccaatcgc gccaagccct accaccacaa caaaatcacc cacgcgcacg
     5701 ttggcatcgc gcacgccgct catggcaaac tgcgccgggt cgtaacaaac ggcgtttttc
     5761 cacgagctgc cttccggcat tttgcgcagc ttgtagttat tcaccgcgtt aatgatgacc
     5821 gtctcggaga gcgggccgta gccgcatacg ctgtcgccca ccgcgtagtc ggtaacgtcg
     5881 ctgccgcact cgataatgtc gccaaccacc atgtttccaa gctggaattt gccaaactca
     5941 atgccgcgcg gcgcatctgc cggacgcgga gtgaacatct gccattcgcc gttaaagtct
     6001 tcatcgataa acgggctggc ggcgcggaag tcgaccactt ccgttccgtg tttcggtgcg
     6061 ccgaaacgca cgcggacctt cacttcatta gctaaaatcg cccgatcttc atactcaacc
     6121 agcgcagcaa cacgcggtgc tgtggctact aactttttca tttaaaactc cttgtaaaga
     6181 aactggccgc tttagccctt cacgccaccg gcggtcaaac cactcttaat aaaacgttcc
     6241 gaaagcgcat acataatcac caccggcaat gcggtcacca gtgaggccgc catcatccgt
     6301 ccccagatgt aatctggcgt actgaacagc gcgttcaggc ccaccggtaa ggtgaaattg
     6361 ccggcgctgg agaggaaaat cgacgcaaac agatagtcgt tccacgccac cataaagcag
     6421 tagacgaaca cggatatcag cccggacatc gccaacggca cggtgatgcg gaagataatt
     6481 tgcagacggt tgaggccatc catcatcgcc gcttcttcga tctcatcggg gatggtgtcg
     6541 aagtagcttt tcagcataaa cacggcggtg ggcagagttt gcgtcaccat ggtgatgatc
     6601 agcgccatct cagtcgacgt aatgctccag cgtccactgc tgcggcagca gcgtaggatg
     6661 cagtgagatc gcctctttcg cgcccttgaa cgaggtcatc agcatcacaa aaaatggaaa
     6721 cagcgtgatg atgagaaaca gcgccagccc gcagtaaaaa ccgatgcgac tgagtgtgcg
     6781 tttatttgtt gccattgagg ttcacccgtt tcctggtcag cagaatgaca gcgaaaataa
     6841 tgatgaagag caccaccgaa atcgccgccg ctttccctaa gtcattaaag gcaaaggcgg
     6901 ttttgtagag atagacgccg agaatatcga ccttggtggt cagcagataa acatcagcaa
     6961 acatgtagaa catccagata gtgcgcaggg tcaccaccgt cgccaggacc ggcataattg
     7021 cgggcagcgt gacgatgcga aaccgctgcc aggcattagc gccatccatc tctgccgctt
     7081 cgtacagcga tttgtcgatg gtctgcaaaa tcgccagaaa cgagataaag gcatacggga
     7141 agtagcgcca gatggcgaac agcaccacca gcacaaagct actgcccgga ttatcgaacc
     7201 acagcggtgc ctgctcatag agatgcagaa gatcgacgcc gaggtagttc acaatgccgt
     7261 agccgttgtt gaacatgtat ttccaggcaa acaccagcga aatggacggc gttacgtagg
     7321 agaggatcac cagcgaacgc gcggttttgc gcaggcggaa ctcgcggttg aaaaacatcg
     7381 ccactgccag cccgagcacg gtgctgcccg ccaccaccag cgcggtatac cagaccgtca
     7441 tccacagcga gtgccagaag ccgggatcgg agaggatacg cacatagtta ctcaccccaa
     7501 caaaggttga ctcgatgttg ggattgagcg gcagacgtaa aaaactgatt tcgatattcg
     7561 acaccattgg ccaggccacc agaccaccca gcagtaataa gctgggcgcg agaagcagca
     7621 gcgcaaaggg catatcggaa cgacctgaaa aaagcctgtt cattgcggat tcctgtcctg
     7681 ggtttagtgc tgttcgatca gttcatccag ctttttctgg ctcgcttgca gcgtgccagg
     7741 gagatcggct ttacccacgg tgacgttatg caccatggag ctcaccacgc cagaacccgt
     7801 cacatcaccc atgcgggtaa agtttttatc ccctactgcg ccaaagacct gaatatttgg
     7861 cagttcaccg attagctgat tcggtagttc acccagcgcc ttaataacgt cgttgtcttt
     7921 ccaggtggcg gtagtgacaa ccgctttatt caccggcagc gcagcacctg gcgacatcat
     7981 cacccagtcg gcaatgttgt ctgcctgctc cataaaggtg acaaattttt ctgctgcttc
     8041 cgtctcttcg gccttttgcc cggcggtaat ggtcagcgag gtcaacatgc cgtagaccgc
     8101 agagtttttc tcggttggca ccacgaaacc gacgtttttc gggtcgcctt ctttaattac
     8161 agccggaagg atataggtgg agtaaatcgc catcggcgcg gtgccgttca taaaggcgtc
     8221 tttcacttcc atgatgtcgt tagaacccgg catgtcgtta gaacccggca tagtgttggc
     8281 agcaaggtcg cggtaatagg tcagtgcctg catcatctct ggtgtatcaa gggtgatttt
     8341 gccttcggcg ttaaagacat tagcctggtt ggataacgca aactgggaga aggattgttc
     8401 cgtcaacacg ctttctgctg taggcagcgc aatgccgtat tttttattcg ccgggtcatt
     8461 cagtttctgt gcaacgtcca gtagctgttg ccagtttttc ggctcctcca gccccgcttt
     8521 tgccagcaca tctttgcgat accagatgcc gccaatccag gcgctgacgg gaacaccggt
     8581 ccaggcactc ccatcttcgg tgcgcacaat acgcagtacg ccatcgtaaa acgcgccttc
     8641 accaacgttg ctgatgaccg tggcaaccgt cttgcgatcg ataagctgct ctttatccat
     8701 aactttggcg tagtcatggc tggtttcgat cacttccggc agcgagccgc tacgtgaaag
     8761 ggtgatgact ttggtgttat aagcgtcctc ttccacgggc acttgtttaa cggtgatacc
     8821 tgggttttct ttttcaaaac gggcaatcaa tttgctgata actgcctgac gctcctgctc
     8881 caccgaagaa tgcataaatt cgatggatac atgggtttta ttttcttctt tacagcctga
     8941 aatcagggcg caggaaacca gtgctgataa cagcacgatt tttgatttaa tcatgagatg
     9001 ttccttttta tttaatccac ataacctgtt aggggcgcag cgttatttca ttacccaata
     9061 gatcaacttc actaatcaga tcccgaccat gcagattagt aatatttaca tgctgaatat
     9121 ttttactgac attaaacaat ccagtcagac aatcaccatc agcgttactt ctttgaatac
     9181 gcattacgga tgaattaatc gtatcgatgg taaaattatt atccggatga aactcgttat
     9241 ggctgcgacg aagtgtaatt aaacgcgaca actcatgata taccgcatgc cttaatgtag
     9301 cttcatcgtt cagttctcgg gttatctctt tactgtgata ttttttacgg ttaatcgcac
     9361 ggttatatcc gagtttttcg acacctgcgt aatcattacg cgagccaaga atactttgaa
     9421 tatatatcgc tggcacaccg gggaaactta acaaaatcgc atgggcgaga ataaacctgg
     9481 cgcaacgttc ttcatcgcta ctctcacggc ggcttaacgc atccatatgt gagaggcgag
     9541 gaagttaaac caggtggtgt tgctggaagg tagtgtcagg ttttgcgccc acgcacaaag
     9601 cgcctcaacg ttctgttttt gcaccgcatg cagcaccagt ggcggcagcg agaactggta
     9661 caccatatgt gcttcgtcat cgcctgcgcc aaagtaagca atgttgtctt tatgcggaac
     9721 attggtctcg gtaatgatca ctgtacctgg cgcaacgtta tcaataatcg accgtaacag
     9781 tttgataatc agatgtgttt tttccagatg gatgcagctt gttcccggct ctttccacat
     9841 aaagccaacg gcatccaggc ggacatactc agcacctttc gcaaggtaac agagtaaaac
     9901 atccaccatc gccagcaaca cttcagggct acggtaattc aggtcaattt gatcgtcact
     9961 aaaggtggtc cataaatggc gcgttgaatg atcgcgcatc tggaatggcg ttaataacgg
    10021 taacgcacgc gggcgagtga cggcgctgag atcggtttgc ggatcaaccg caataaaaaa
    10081 atcttcaaaa cctggctgct gttgtaaata gtttttaaac cattcacttt ttgccgacat
    10141 atggttgcag acaaaatcaa acattaaatg actgcattca ccgagttgct gaatatcctg
    10201 ccactccccc gcttcactgg cgacctgatg ataatcaatt accgaaaacc catcatcaga
    10261 tgaacatgga taaaaaggta ataaatgaac atgtgaaaaa atgctttgca gccattgatg
    10321 gtaaaactga ttaaatgtgg gtaatggttt taaatcattg ctgtgaaatt gatcggcata
    10381 ggtaattaac acgacatcac tttcatccca gtgtttttta cgtcgctgtg taattaatcg
    10441 tttcgcactc tcaagacgcg ttacaagttt ctgtaaatga gttgcggtaa atgttccacc
    10501 gtagatttcg tccaggtaat ccgtaatttt ctgtttcatg ttgccttttt gtggtagcgg
    10561 tcccatgaaa cgagagacta ctccgcagaa aataaactgt caattgagca caagggtttt
    10621 agtcacgcag gaaatcattt tttaccaggg aaaaagcgta tttgtgagct gacgcaaata
    10681 atctatgggg atgtgaataa ggtgtgacag aaaaaaaacg gcgcataagc gccgctcatg
    10741 gtgaattctt ttaacctttg accttcggca ttgcgatgtc tttcaggcca ccggcgttct
    10801 caacgtgggt atatcccatc tcgctaagga tctcttttgc ttgccctgac tggcgtccgg
    10861 cattgcaata cactttcacg gtgtcgtttt tatccggaac ggcggtggca atgcgctctt
    10921 tcacttcctt caggggaata ttgatggccc cctgaacgtg ctcttgctga tactgctctg
    10981 gaacacgaac atcgatccag tgttcagcgg cgaaaacggg cagtgaaaac accagtgcca
    11041 gagcaagtaa gcctttttta aacatgtaag cgtccttcta tggatgaaaa ttgccatcca
    11101 ttatacgcct gagatttaac aatttttgcg aaactcccct caccttttat aacgctgtgc
    11161 cagtttatta gcagcccgac ttaacagcgg ttccagcgcc acagccagca acattttcag
    11221 cggtcggcga gcaactgatt ttatcgccca gcccgctaca cccgccgggc catagcgcag
    11281 tgcggtaaga agtaccagct tgcctgctaa tttgaaacca ggctttacct tttgcccggc
    11341 ctgttgccag cgagtattca taactttcct cacagttgac ggaaacggct acgtaacgtg
    11401 aaagtatcgg aagtgacata acgttccatc tcgcgtaaac gcgtttcact tgccgccagt
    11461 tcacggtcga cttcatccag caattcgctg ctggaaggta gctgctcacc aaaggccatg
    11521 ttgtccggca ttggatcaag cgcaaatgac aaaatgatgt aagcaaccag ggtaaacagc
    11581 gccagaccga agaaaatcga cagcaccacc aggatacgca ccagttttac cggtacatca
    11641 aaatagttgg caatcccggc gcagacgccg cggaccatgc cctgctgtgg aatacgccat
    11701 aattttttat tgagattaat gcccgccatt agcgatccct ccagttcgga tgttctgcat
    11761 caagaataga ttccagcgcc tgaatacgtt cgcgcatccg ttttgcttca tcagccagtt
    11821 gcgctaatcg ctgctgctca ctttgcgaca attcatcgcg accagaacga ttgctgtaat
    11881 gcagccataa ccagatcggt aaaacaaaca gcacaaaaat ggttaacgga atagccagaa
    11941 atagcgcgct cataagtact ccttagacga tgagcggcgg cgcgttcgga cgccgcctgg
    12001 atgttattat tgattgtctt gcttcatttt ggcttttaat tgtgccagtt gttcgctgat
    12061 tgcatcatcg gctttcagtt cggcaaactg atcgtccagc gatttttgtt taccgaagct
    12121 gtggctttct gcttccgctt ccatctggtc aattcgacgt tcgaaagatt cgaaacgagc
    12181 cattgcttca tccagtttgc cactgtccag ctgacgacgc acatcgcgcg acgagtttgc
    12241 cgcctgatga cgtaacatca atgcctgctg gcgagcgcgt gtttcgctca atttgttttc
    12301 cagctcgcca atctctttct tcatgcgtgc cagcgtatcg ttcaccagcg tcacttcatg
    12361 ttccagggac ttaatcagat cggtcagttt ctgtttttca attaacgctg cacgtgccag
    12421 atcctctctc tctttcagca gcgccagttc ggctttttcc tgccattcaa cttcacgcgc
    12481 cgacgcttgt tcaatacggc gagtcagctg tttcttttct gccaacgcac gcgccgaagt
    12541 agaacgtact tcaaccagtg tatcttccat ctcctggatc atcagacgca ccagtttctg
    12601 tggatcttcc gctttctcta acagagcgtt gatgttggca ttcacgatgt cggcaaagcg
    12661 agaaaaaata cccataatgt tgtcctcttg atttctgcgt tcaggattgt cctgctgaac
    12721 tgttaataca atttgcgtgc caatttttta tctttttgat ttataaagat ctgattgaag
    12781 aatcaacagc aacatgccaa gatgaattag cgaattacac taacaagtgg cgaatttcat
    12841 catggccgaa tacaaagata atttacttgg tgaggcgaac agctttctcg aagtgctgga
    12901 acaggtttcg catctcgcac cgctggacaa accggtgctc atcatcggcg aacgcggcac
    12961 cggtaaagag ctgattgcca gccgcctgca ttatctctcc tcccgttggc aagggccgtt
    13021 tatttccctt aactgcgcgg cgttaaatga aaatctgctg gattccgaac tgtttggtca
    13081 cgaagcgggg gcgtttaccg gtgcgcaaaa acgtcatcca gggagatttg aacgtgccga
    13141 cggcggtacg ctatttcttg atgaactcgc tacggcaccg atgatggtgc aggagaaatt
    13201 attgcgcgtg attgagtacg gtgaactgga gcgcgttggc ggcagccagc cattgcaggt
    13261 gaatgtgcgg ttggtatgcg cgacgaatgc cgatctcccg gcgatggtca atgaaggcac
    13321 ttttcgcgct gacctgctcg accgactggc ttttgatgtt gtacaactgc caccactgcg
    13381 cgagcgcgaa agcgacataa tgttgatggc agaacacttt gccatccaga tgtgtcggga
    13441 aatcaagctg cctctgttcc cggggtttac ggagcgcgcc agagaaacat tgctgaatta
    13501 tcgttggccg ggaaatattc gtgaattgaa aaacgcggtg gaacgttcag tgtatcgcca
    13561 cggcaccagc gattatccgc ttgatgacat cattattgat ccctttaaac ggcgtccgcc
    13621 tgaagacgct atcgccgttt cagaaaccac ctcgcttccg acgctgccgc tggatttacg
    13681 tgagtttcaa atgcagcagg aaaaagagtt gctgcaactc agtttgcaaa aggggaaata
    13741 taaccagaag cgcgcagctg aattgctggg gttaacctac catcagttcc gcgcgttgtt
    13801 gaaaaaacac cagatttagc gaagttgggc aatgcgattc gccggatgcg gcgtgaacgc
    13861 tttatccggc atacataatc aacgtcgttt gcaggcctga taagcgcagc gcatcagaca
    13921 ttattgcgtt gggctattaa tcgctcagcg catcctgcaa aattttcatt gccgcatcga
    13981 attgcgcatc cgggatggtc agcggataca ggaagcgaat cacgttgccg tatgcgccac
    14041 aggtcagcag gagcagcccc tgcgccagcg cgcgttgctg gattttctgt gcaatcgccg
    14101 ctgacggctc gcccgtttgc ggatcgttaa actctgccgc aatcatcgac cccaggccgc
    14161 gtaccgcagc aatggccgga acgctttctt tggcatcaat caacgtgttt ttgagacgct
    14221 ggcccagttg attcgcgcgt tcgcagagtg attctttgtt gataatattg agcaccgcgt
    14281 gcgcggca
//
LOCUS       DAJTZL010000105        14276 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.105, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000105 DAJTZL010000000
VERSION     DAJTZL010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14276
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.105"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            92..1132
                     /locus_tag="OO544_002711"
     CDS             92..1132
                     /locus_tag="OO544_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HCR6768272.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLHTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(1164..2357)
                     /gene="lplT"
                     /locus_tag="OO544_002712"
     CDS             complement(1164..2357)
                     /gene="lplT"
                     /locus_tag="OO544_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HCR6768273.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETMSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVMGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(2350..4509)
                     /gene="aas"
                     /locus_tag="OO544_002713"
     CDS             complement(2350..4509)
                     /gene="aas"
                     /locus_tag="OO544_002713"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HCR6768274.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVEMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            5095..6126
                     /gene="galR"
                     /locus_tag="OO544_002714"
     CDS             5095..6126
                     /gene="galR"
                     /locus_tag="OO544_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HCR6768275.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLVVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIAANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(6133..7395)
                     /gene="lysA"
                     /locus_tag="OO544_002715"
     CDS             complement(6133..7395)
                     /gene="lysA"
                     /locus_tag="OO544_002715"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HCR6768276.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            7517..8452
                     /gene="lysR"
                     /locus_tag="OO544_002716"
     CDS             7517..8452
                     /gene="lysR"
                     /locus_tag="OO544_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HCR6768277.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(8439..9086)
                     /locus_tag="OO544_002717"
                     /pseudo
     CDS             complement(8439..9086)
                     /locus_tag="OO544_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase"
     gene            complement(9217..10635)
                     /gene="araE"
                     /locus_tag="OO544_002718"
     CDS             complement(9217..10635)
                     /gene="araE"
                     /locus_tag="OO544_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HCR6768278.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
     gene            complement(10950..11711)
                     /gene="kduD"
                     /locus_tag="OO544_002719"
     CDS             complement(10950..11711)
                     /gene="kduD"
                     /locus_tag="OO544_002719"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HCR6768279.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIKQVTALGRRFLSLTADLRKIDGIPALLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQRNGGKIINITSMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPIVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(11776..12612)
                     /gene="kduI"
                     /locus_tag="OO544_002720"
     CDS             complement(11776..12612)
                     /gene="kduI"
                     /locus_tag="OO544_002720"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HCR6768280.1"
                     /translation="MDVRQSIHSAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            complement(12899..14080)
                     /locus_tag="OO544_002721"
     CDS             complement(12899..14080)
                     /locus_tag="OO544_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HCR6768281.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
BASE COUNT         3703 a         3915 c         3391 g         3267 t
ORIGIN      
        1 ccggctggcc ttaagatttt tctctttccc ttttcccttc cctctgccat ttttatattc
       61 cttatgtcgt gattataaaa aggaaacggc tatgcaatat caccgtatac cccacagttc
      121 gctggaagtc agcacgctgg ggcttggcac gatgacgttt ggtgaacaga acagcgaagc
      181 cgacgcccac gcacaactcg actatgccgt cgctcagggc attaacctta tcgacgttgc
      241 cgaaatgtac ccagtacctc cgcgccccga aacgcaaggg ttaaccgaaa cctacgtcgg
      301 caactggctg gcgaaacatg gcagccgcga aaagttaatt atcgcctcca aagtgagcgg
      361 accgtcgcgc aataatgaca agggcatccg cccggatcag gcgctggatc ggaagaatat
      421 ccgcgaagcg ctgcatgaca gcctcaagcg cctacacact gattacctcg atctttatca
      481 ggtgcactgg ccgcagcgcc cgaccaactg cttcggcaaa ctcggttata gctggacgga
      541 ttctgcgcct gcggtttcgc tgctggatac gctggacgca ctggcagagt accaacgcgc
      601 gggaaaaatt cgttatatcg gcgtgtcgaa cgaaactgca tttggcgtaa tgcgctacct
      661 gcatctggcg gacaaacacg atctgccgcg tattgtcacc attcagaacc cttacagtct
      721 gttaaaccgc agttttgaag taggtctggc agaagtaagc cagtatgaag gggtcgaact
      781 gctggcctat tcgtgcctag gtttcggcac gctgaccggg aaatatctca atggtgcaaa
      841 acccgctggc gcacgtaata cgctctttag tcggttcacc cgctatagcg gtgagcaaac
      901 gcaaaaagcc gtcgcggcgt atgttgatat cgccagacgt catggcctag accctgctca
      961 gatggcgctc gcgtttgtac gccgtcaacc gtttgttgcc agcactctgc tgggcgcaac
     1021 cacgatggat cagctgaaaa ctaacatcga aagtttgcat ctggagttaa gcgaagacgt
     1081 attagctgaa attgaagcgg tgcatcaggt ttatacttat ccggcaccat aagattaacg
     1141 ccggttaaaa ccggcgttaa atattaatga cggcgctgcc agatccacag cgccgttatt
     1201 gccagcgcaa acagcgcacc gaagccaatg ccaatgggca cgaccgggat gcctaccatt
     1261 accgccagcg agtaaatgcc cagcatcaac aacatggcgc tgttttcgcc aaggttttgt
     1321 actgcaatcg cattccccgc cccgacgctt tttttacccc gctcctgtag caacgcattg
     1381 agcggaacga caaaaaagcc ccccatcacg ccaatcagca tcaacaaggc ataggctggc
     1441 agcagctcgt gttgcaggga aaaaatcagt accaccacgc caatcaaaat cccggctggc
     1501 atacagcgtg acatggtttc cagcgtaact aacttcgctg ccgcacctgc gccaaccacg
     1561 ataccaatcg ctaccatcgc gttgagatag gtgggcgtag cgttatcggt aatgcccagc
     1621 gccaccggta cccacagcac caacaggaaa cgcagcgtga cacccgctcc ccagaataaa
     1681 ctggtgccca ccagcgaaaa acgcgtttca ccattgcgcc atagcgaggt gcaggcattc
     1741 aggaaactgc gggtcatgtt gatgagattc caggactgcc ccggacgcgc cgccgccagt
     1801 ttgggaatgt agatattggc aacgaccgca ccaccgtagg ccagtgcgca tgcggccagg
     1861 gcgacgagga catgccagtc agccagcaca ccaccggcta cggaaccgag caaaatcgcc
     1921 gctatggtag aagcttccat taaaccgtta gctttcacta acttactacc cgtggttaat
     1981 tcgccgagaa taccgtattt cgccggtgaa taggctgcag caccaacacc caccagcgta
     2041 tagccgagaa acggattgat accaaagcag atactggctg cgcccagcag cttcaggccg
     2101 ttggcaaaca tcatcacccg gcctttggcg aagctatccg ccacctgccc gacaaacggc
     2161 gcaaaaagaa tgtaagcacc tacaaacacc atttgcagga tgggctggct ccactccgga
     2221 tagaactgcg ctttcagtaa cgccagagtg gcaaacagta gggcattatc gccaaacgca
     2281 gagagaaact gcgccacgat aaccgctttc atccccttcg accacaacga agtgttagtg
     2341 tgcactgact cactcatcgt gttgttccgc ttcgtctacc cagcttttca acgtgacaaa
     2401 gtcaggtttg ccgctgccaa gtaatggcat ctgtttcaga tagcgaatat cgcgcggtac
     2461 agcaagctcc ggtacgccgt gctcgcgggc atactgttgc aacttatcgc gcgtcagttc
     2521 gttatctgtg gtgaaaagca ccagtgcctc gcctttgctg gcatcgctct taatcgcagt
     2581 ggcatggact ttatctggcg aaacaccaag tgccagttgt tccaccattt ccagcgacac
     2641 catttcgcct gcaattttgg caaagcgttt tgcgcggccc tgaatctgca caaagccctg
     2701 ctcgtcaaaa cgcacaatat cgccagtgtc ataccagccg cgctccattt cgccgcgaac
     2761 attctcggcg gtgggcactt ccagtacacc tggcttctcc acccgcagat agccgttcat
     2821 tatgttcggc cctttcagtt gcaggcgtcc gccctcttcg ataccaggga ccgacaacag
     2881 gcgcgcatcc attcctggta gaatacgccc taccgtaccg ggtttcgccg ccatcggtac
     2941 gttgatagaa acgacaggcg cgcattcggt cacgccgtag ccttcaagga tgcgcaggcc
     3001 aaatttatcc tgccaaagct gtttggtact ttcttgtaat ttttctgcgc ctgccaccac
     3061 atagcgtaga cgatagaagt catacgggtt ggcgaaacgc gcgtagtgac cgaggaaagt
     3121 cgaggtgccg aacaacacgg tgcaactgcg gtcatacacc aactccggca caatacggta
     3181 atgcagcggg cttggataaa gaaacacttc tgcacctgtg agcagtggcg taaacaggcc
     3241 taccgtcagc ccaaaggagt gaaacagcgg taacgccgac ataaagcgat cgttggtggt
     3301 gaagtcggca atcgttttaa tctgctcgac gttcgccaga atgcttttat ggctatggac
     3361 gacgcctttc ggatggcctt cagaaccgga ggtaaagagg atcagcgcct cttcttccgg
     3421 ctgctgtttt acctgtgcca gacgtggcat cagcagatgc gcgaagatcc atactttgtc
     3481 ggcagtggtg acatctgctt ttaaatcttc cagatagacc cagcgcacct gagtaagttg
     3541 ctccggcaga tgccagagtt tgcctttatc gagaaactgg cgggaagtga agatggtttt
     3601 gatttcagcc gccgtaatag cactggtcag cccttttacc ccggcagtgt agttcatcat
     3661 tgcgggaatg cggcgacggg cgatggcccc aaaaatcact gccgcactga tgcctgcatt
     3721 gggcagcatt aagccgatgc gttcgccttc aacactgtat ttttcaagga tgcgtccaac
     3781 aaacagcgtt ttcgtaagca atttgcgata ggagtctggg gtaaagttga cgtcttcgac
     3841 acatttcttc ccggctccga agcggtacat tgcactcagt aaagattcgt acagcgtttc
     3901 acgcgggcgc accgccattc gcgcttccat cattatttga tgcagcattt cgccagcgat
     3961 tttgcgacgg tcacgggcac gcggcgcatc cggcatctcc acctgcgttg gtggcaaaat
     4021 atgcagagta atttgcggga acaagcgacg tttaaccaga cctttcaggc ggctgaagtg
     4081 cgtaagttcc gccccttcaa tacgcacagg aataaccgtt gcaccagact tcgccgcgac
     4141 aaaacccgcg ccatcgtaga ttttcatcag cgagcctgtg gtggtgatgc gtccttcagg
     4201 gaaaatcacc actggtcggc cctgttccac cagacgtacc agatgtttaa tcgccatagg
     4261 ttgcgtcggg tcgagaggaa caaagtcgat aaatgatttc agccaacgca tataccactg
     4321 ttggcttatt gaggtgtaaa cggcaaacac tggacgcaca ggtaaaaaca gtccaagcaa
     4381 aatgccatca ataaaagaga cgtgattagg cgtaattaga acgcgctcgc ccttcagtgc
     4441 ctgggtgtca cccgtaacgc gaacgcgata caaaacacgg cacaaatttc gaaaaaagct
     4501 aaaaagcatt ccaactccct ttgctctgat tcagtaaaag cgaatggagg gagattacac
     4561 gagataaaga acgcgagcga cagtaaatta ggtgcgaaaa aaaacctgcg catccgcgca
     4621 ggttggtgca agagacaggg tacgaagagc gtaccgaata atctcaccaa tcaatacctc
     4681 tgggatcttg attgtggtct gcacgacgac tcttcgccag cgagaaaacg caaaggaatg
     4741 aagggaaatg caacgaggtg tgtaaattgt cggttactgt tacagattga tgaccggcaa
     4801 aaaaaaacct gcgcatctgc gcaggctggt gtaattcatg tgctcaaccc gaagttgact
     4861 tcacctatca atacctctgg gatcaccact ttagcaacct gaagccaaac gccaccagca
     4921 gtcaatcgca acagcatttc gcaaagtgta accaaaggtt tgcaatctct ttttttgtct
     4981 tacttatttg cgtttggctc acattcccac gatgaaaaca cgccgcccct tgaaccaacg
     5041 ggcgttttcc gtaacactga aacaatgtaa gcgtttaccc actaaggtat tttcatggcg
     5101 accataaagg atgtagcccg actggcaggc gtttcagtcg ccaccgtttc ccgcgtcatt
     5161 aataattcac ccaaagccag cgaagcgtcc cggctggctg tgcatagtgc aatggagtct
     5221 cttagctatc acccgaacgc caacgcccgt gcgctggcgc agcagaccac tgaaacggtc
     5281 ggtctggtcg ttggtgatgt ttccgatccg tttttcggtg caatggtgaa agcggtcgaa
     5341 caggtggctt atcacaccgg taatttttta ttgattggca atggttacca caacgaacaa
     5401 aaagagcgtc aggccattga gcaactgatc cgccatcgct gtgctgcgtt ggtcgtccat
     5461 gccaaaatga tcccggatgc cgatttagcc tcattaatga aacaaatgcc cggtatggtg
     5521 ctgatcaacc gtatcctgcc tggcttcgaa aaccgttgta ttgctctgga cgatcgttac
     5581 ggtgcctggc tggctacgcg tcatttaatt caacaaggtc atacccgcat tggttatctg
     5641 tgctctaacc actctatttc tgacgccgaa gatcgtctgc aagggtatta cgatgccctt
     5701 gctgaaagtg gtattgcggc caatgaccgg ctggtgacat ttggtgaacc agacgaaagc
     5761 ggcggcgaac aggcaatgac cgagcttttg ggacgaggaa gaaatttcac tgcggtagcc
     5821 tgttataacg attcaatggc ggcgggtgcg atgggcgttc tcaatgataa tggtattgat
     5881 gtaccgggtg agatttcgtt aattggcttt gatgatgtgc tggtgtcacg ctatgtgcgt
     5941 ccgcgcctga ccaccgtgcg ttacccaatc gtgacaatgg cgacccaggc tgccgaactg
     6001 gctttggcgc tggcggataa tcgccctctc ccggaaatca ctaatgtctt tagtccgacg
     6061 ctggtacgtc gtcattcagt gtcaactccg tcgctggagg caagtcatca tgcaaccagc
     6121 gactaaccgc agttaaagca attccagcgc cagtaattct tcgatggtct ggcgacggcg
     6181 aatcaaccgc gcctgaccat tatcaaacag aacttctggt aacagcggac ggctgttgta
     6241 gttggatgac attgatgcgc catatgcccc tgtatcatgc agtaccagat aatcacccgc
     6301 cttcacttcc ggcaaggcgc gggtttcaac atttccccct tcctgctggg taaagacatc
     6361 gcccgattca cataatggcc cggcgacgac ggtttccacc gttggcgcgt gttccagaga
     6421 acgaccatca gctgccaggg cactgatatg gtggtaacta ccgtacattg ccgggcgcat
     6481 cagatcgttg aacccggcat caaccagcac aaagtggcgg ctacccattt gtttgacgct
     6541 ccgcacctga gtaattaaca cgccagactg cgctaccaga aagcgacccg gttcaatttc
     6601 cagtttcaca gggtggccca aatggcgagc gatttgctca cgcgcggcat tccacagacc
     6661 ataataatgt tcggtatcaa ccgcctcttc accctgttga taaggaatag aaagcccacc
     6721 gcccgcagaa atagcctgta aatcctgacc gaattcgatg acctgacgta ccatagcacc
     6781 acacacctgt tccagatggg cataatcaac gccagaacca atgtgcatgt gaatgccgac
     6841 cagctgcaga tgatgacgtt gtatcacgtc cagtgcggcg ggcagatcgg tgtaccagat
     6901 accgtgcttg ctgttttcgc caccggtatt ggttttttgg ctatgtccgt gaccaaaccc
     6961 cgggttaacg cgcagccata cccgatgccc tggcgaaacc tggcccagtt ggtcgagcat
     7021 atcaacagaa cccgcattca ccggaatttg caattcactg acgcgttcaa gcgtcgcctg
     7081 atcgataaca tctgccgtaa aaacaatatc atcggggtgc gtttgcggat tgtaacccgc
     7141 cgccaacgca cgctctattt cgcctaacga gacggaatcc actttcacgc cctgctcacg
     7201 cattaatcgc aaaatatgaa tattggaaca ggctttctgt gcaaagcgca ccacatcaaa
     7261 ctgtttcagc gctgcaatct gccgacgaat aatttgcgca tcgtagaccc acaccgggca
     7321 gccaaattca gcgggcaaac gcagcagatt ttcggcggtg agatcggtat cggtgctgaa
     7381 cagtgaatgt ggcataacaa actccagatg agtgcttttt tatgattacg ccacatcata
     7441 aaaagaataa aaaatatcga tttatgtcga gtctatgcaa aaatgatata gattaccgga
     7501 ttgcgagaga gcgctaatgg ccgccgttaa cttacgtcat attgaaattt ttcatgcggt
     7561 aatgaccgcc ggaagcctga ctgaggcagc acacctgcta cacacctcac agccaaccgt
     7621 cagccgcgaa ctggcgcgct ttgagaaggt gatcgggcta aaattgtttg agcgcgtacg
     7681 tggacgatta catcctaccg tgcaaggact gcgtctgttt gaagaagtgc aacgatcctg
     7741 gtacggactg gatcgcattg tcagcgccgc agaaagtctg cgcgagtttc gccagggaga
     7801 actgtctatt gcctgcctgc cggtcttttc gcaatctttt ttaccgcagc tcctgcaacc
     7861 ctttctggca cgttatcccg atgtcagctt aaatatcgtg ccccaggaat caccgctact
     7921 tgaagagtgg ctctcggccc agcgtcatga tttaggactc actgagacac tccatacgcc
     7981 tgcgggaaca gaacgtaccg aattactctc tttagatgaa gtgtgtgtgt tacctccggg
     8041 tcatccgctg gcggtaaaaa aggtattaac gccggatgat tttcagggtg agaactacat
     8101 cagcctttcc cgtactgaca gctatcgcca gttgctggat cagctattta ctgaacatca
     8161 ggttaaacga cgcatgatcg tagaaaccca cagcgccgcg tcagtctgcg caatggtacg
     8221 ggcgggggta ggtgtttcgg tggttaaccc actcaccgca ctggattatg cggcaagcgg
     8281 tttagtggta cggcggttca gtattgcggt tccgttcacc gtcagcctga tccgccccct
     8341 gcaccgcccg tcatcagcgc tggttcaggc gtttagtgag catttacaag cggggttacc
     8401 gaaactggtc acttctcttg acgcgatttt gtcgtcagct acgacagcat aaaagcgaca
     8461 gcatcctcgg catggatcgc cgcagtatca aacacaggca aaacactgcg ctcttctgga
     8521 accaataaac caatttctgt gcagccaaaa atgacaccct gcgccccctg ttctgcaagg
     8581 cgagcaatca cttgcgcata ataagcgcgt gacgcttcgg taaattgccc cagacacagt
     8641 tcttcaaaaa taatctgatt aatcttcgcc cgttcatccg cttccggaat aagacaattg
     8701 atggaaaatt attccgtcag ccgcccgcga taaaaatcct gttccatggt gtaacgcgta
     8761 cccagcagcg ccacacgagt cattccggcc ccggtaattg cacgtccggt ggcatccgcc
     8821 actttatgca tcgtattggt acataacaca ataccttctg cgcccgcccg ctgtaagcca
     8881 agcgccgcct cagccagaat gtccccggtt ttatcccatt ccccgcgacg ctggcactct
     8941 tctatttcat gaaaatcgac gctatgtagc agcacttgcg cagagtgaag cccaccaagc
     9001 cgctgtttaa tgccttcatt tatcagacga tagtaaggaa tggtggattc ccagctcatt
     9061 cctcccagca aaccaattgt tttcattcct ctctctcctg atgtgtgtta ccgcaacaag
     9121 gctgaataag gaacgagaca aacgcctcaa cggccaggtg cccaatctct attaacgaaa
     9181 aaagggccgg atgtacagca catccggccc gtgaaatcag acgccgatat ttctcaactt
     9241 ctcgcctgcc atcagtttgc gttcgatatg ttccagcgtg acatttttgg tttccggaat
     9301 gagccagaaa gtgatgccca caaacgcaat gttcagcgca gtgtagagcc agaacgtacc
     9361 ggcagcacca atgctgtcga gcagcgtcag gaaggtcgcg ccgataatca tattcgacac
     9421 ccagttcgtg gtggtcgaac aggtaatacc gaaatcgcgg catttcagcg gctgaatttc
     9481 agagcacagg atccacacca ctggcgcggc gctcatcgca taaccggcaa tacacatcat
     9541 cgtcatgcca acagagagcc aggacaagcc actggaagcc gtaccgttat caaactgcat
     9601 caggcaatag cccagcacca gagtgcctaa cgccatcacg ctgaaaccaa ttttcagagc
     9661 cggtttacgc cctgctttat ctaccgtaaa caccgcaata aaggtggcga acataaaggt
     9721 cagccctacg accagagtcg caatcatctg ttgttctgtg gtcgtaaagc ccgccatttt
     9781 gaagatacgc ggcgcgtagt acatgatgat gttcataccg gtaaactgct gcatcgcctg
     9841 caacaacata ccgagaaaca cagcacgacg gacgttacgg ttgatcttaa acagtgccca
     9901 accgccctgt tttaacttca ggctttcacg aatttcgttg agttcttctc gcgctttttc
     9961 cgacgtatcg cgcagcatac gcaacacttc ttccgcctca atatgacgcc ccttttccgc
    10021 cagccagcgc gggctatttg gcaggaagac caccagaata atcagcagga ctgctggtaa
    10081 agcaagaacc cccaacattg cgcgccagtt accgctataa ctgaacgctg tatcggataa
    10141 aaacgccagc acgatgccga gtgtgaccat caactggtac atactgatca tcttaccgcg
    10201 aacgttttca cttgccattt cagaaagata cagaggagcg gtgtaagacg cgatcccgac
    10261 agcaatgccc agcaccaccc gagcggcgat taacatctct acgctggtcg caaaagcgga
    10321 ccctatagaa ccgagtacaa acaggatggc ccccgccatc aggctgtatt tacgccccag
    10381 gcggaacgac agccaaccat taaacagcgc accaattgct gcgccgagca tcatgctact
    10441 aaccacccat tcctgcaaac gactggtcag cacaaagtga tcggtaatga acggcaacgc
    10501 tccggcgatt acgccgatat caagaccaaa taacaatcct gcgaccgcag cagctaccga
    10561 aacaaacata ttcatacgcc gcgtatcccg caaagaacgt ggcgttaaag cagattccgt
    10621 attgatagta accatttttt cctgccagca gagagtaaga catagtgaaa aaatacgtga
    10681 acaactcaca caggtgtcag gtcggaaaca gcataaatat ggattaaatt gctgcgacat
    10741 gtcgttatgt gatggatatt ccaattttca aattaagttg aattattgag attattatta
    10801 accacctaat tttacagcag ataaaattca taaagttcat taattgataa ttgatatgga
    10861 ttatttcata accatgatat gaattatgat gacctacagg tataaaaaac cctgccatga
    10921 ggcagggtca taaaagtaag aggaatgaat taacgagcca gccaaccgcc atctacggca
    10981 atggtataac cattcacata atctgaagcg ctggaggcaa ggaacactat cggccccatc
    11041 aggtcactcg gcagtcccca acgaccagct ggaatgcggt cgagaatttc cgcgctacgt
    11101 tgttcatctg cccgtagttg ttgagtattg ttggtcgcca tgtaacccgg agctatcgca
    11161 ttaacattaa tgttgtgttt agcccattcg ttcgccatca atcgcgtcac gcccatcacg
    11221 ccgctttttg atgcggtata agaaggcaca cggatcccgc cctggaagga gagcattgac
    11281 gtgatattga taatcttgcc gccattgcgt tgcgcgataa agtgtttcgc cgctgcctga
    11341 gacatgaaga atacgctctt gatattcagg ttcatgacat cgtcccagtc tttttcgctg
    11401 aactcgagag catcttcgcg gcgaatcaat ccggcgttat tcaccaggat atcaatatga
    11461 ccaaactcag ctaccgcgcg atccagcagt gctggaatgc catcaatctt tcgcagatcg
    11521 gcggtcaggc ttaaaaaacg acgccccagc gccgtgacct gcttgatggt ttcagtcggt
    11581 tcaacgatgt taatgccaac aatgtcacag cccgcttgcg ccagccccaa cgccatcccc
    11641 tggcccagtc cagtatcaca accagtgacg accgcaactt taccttcgag agaaaatgca
    11701 cttaaaatca taacaatacc ttattcgttt atgccgacaa ctagcgcaaa tctttaacgg
    11761 ccactatgcc gacaactagc gcaaatcttt aacggccaca tggtccatat catcaaagac
    11821 ctggttttca ccaaccatgc cccagataaa ggtataagct ttggttccga cgccggaatg
    11881 gatcgaccag ctgggggaga tcaccgcctg ctcgttatgc atcacaatgt gacgcgtttc
    11941 ttgcggctgc cccatcatgt ggaaaacgca ggcgtcatca tccatattga aatagaaata
    12001 aacttccatc cggcgctcgt gagtgtgaca cggcatggtg ttccacaagt tacctggagc
    12061 cagctccgtc agccccatac tcaattggca ggtttccagc acatccggga caaaatattt
    12121 gttaattgtg cgacggttac tggtgaggtt atcgcctaac gtgactggag atacttcgtc
    12181 cggtgtgact tttttggtgg gatacgtcgt atgcgcgggt gcgcaattgt aataaaactt
    12241 tgccggagtg gcggtatcaa tactggcaaa gacaacttct tttgcacctt taccaacata
    12301 cagggcgtcg cggtgaccga tttcatagca ttggccatcg acagtaatcg taccggcacc
    12361 gccaatattg ataacaccta actcgcgacg ttcaaggaaa tagcttacgc cgagttgttt
    12421 accaacttcc ccgccaacgg aaaccgtttt agttatcggc ataatgccgc caacaataat
    12481 tcggtcaatg tggctgtaaa ccatggtgta ctcatcggcg acaaatacct tttcaaccaa
    12541 aaattcattg cgcagccctt gggtatccag cgtttttgcg tgcgcactgt ggatgctctg
    12601 tcttacgtcc acatcaacct ccgataatca gtgaagcgtg aaaatagaaa acgatagtga
    12661 aacactgttt cgttttctct ataaagggat gatatgccca tgggctaggg ctttcaatta
    12721 aaaataaaac aatgtttcat ttttaagtta aggattaaaa aaagtgcagt gtcgatcacg
    12781 aatatgtcat gagaatggaa aaatgaggga gcccagaaaa tgtatgtcga cttgccagcc
    12841 atcattattc aacagattaa tgacggcaga tatttatcgg taaggaggaa atgcagagct
    12901 actcgtcacg ttcaatggtc aatgccacgc cctggccccc gccgatacaa agcgttgcca
    12961 gccctttgcg ggcattacgt ttcaccattt catgaaccag agaaaccagg attcggcaac
    13021 cggaagcgcc tatcgggtga ccgagtgcga tcgcgccacc attgacattg acccgacgct
    13081 catcccactc aagcatcttg ccaaccgaaa gcgcctgtgc agcaaacgct tcattagcct
    13141 cgataagatc gacatcagcc aactgccagc ctacacgctc caggcaacgg cgggtcgcat
    13201 acaccggcgc aattcccatc aatgccggat ctacaccaac gctggcaaat gcgcggatgc
    13261 gggccagcac gggtaaattc aacgctcgtg ctttggcttc gctcatcatc attaccgcag
    13321 ctgcgccatc gtttatggat gatgcattcc ccgcggtcac agaaccgaga ctatcaaatg
    13381 aaggatttaa acgggctaaa ccttctgcac tggcgtcagt gcgtggctgt tcatcggtat
    13441 caacaaccaa cgtctgaccg ttactttgga tcattaccgg gacgatctca tctttaaatc
    13501 gtccggcgtc aatcgccgct cgcgcttttt gttgcgagct aagtgcgtaa gcatcctgca
    13561 actgacggct gatgccatat tcgcgagcca gattttcggc ggtgacacca atatgataat
    13621 cattgaaggc atcccacaac ccatcatgca ccagactgtc aaccaactgg ctattgccaa
    13681 gctgtgcacc ggtgcggcta tcagtcagaa catgtggtgc gcggctcatg ttttcctggc
    13741 caccggcgat gacaatatca gcctcgccac actgtatcgc ctgagtagcc agatgcagtg
    13801 ctttaagtcc ggaaccgcaa acgtcattaa tcgtgattgc agaaacgcta ttaggaagac
    13861 cacctttaat agccgattgc cttgccggat tctgccctgc ccctgcagtc aacacctgac
    13921 caagaattac ttcatccacc gcatatgcag gaacgccggt acgttctatt aacgctttca
    13981 cgaccagact accaagttcc acggcggaat gacccgctaa cgcaccacga aagcagccga
    14041 taggtgtccg taacgccccg acaatcacaa cgtctttcat cacaacctcg caccaattaa
    14101 cagtgcaata gtaaatgatt gttaattaca gttatcttaa ttgtttaaaa aaagtgattt
    14161 ttatcacaaa ggaaatatgc ctgagcagta gtcagagtca taactggcac gtaaggtttg
    14221 caaccactaa cccaccaata gatgggtaga taaggcgtta atctcccata cttaac
//
LOCUS       DAJTZL010000106        14159 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.106, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000106 DAJTZL010000000
VERSION     DAJTZL010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14159
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.106"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..228)
                     /locus_tag="OO544_002722"
     CDS             complement(<1..228)
                     /locus_tag="OO544_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3289 family protein"
                     /protein_id="HCR6768282.1"
                     /translation="MNNILLPPINIPCTLFETISLFDDFSADDMQYGDMVEQDFLSLG
                     LSDISAKVDPYRLIKYHFPGPGSINVAFSASS"
     gene            385..561
                     /locus_tag="OO544_002723"
     CDS             385..561
                     /locus_tag="OO544_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001351582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA"
                     /protein_id="HCR6768283.1"
                     /translation="MLNLPIRKLFPTRIASSYGMNFEFMPELKWSFGYPGAIIFMILA
                     GLTPYLYFKRKNWL"
     gene            1187..1486
                     /locus_tag="OO544_002724"
     CDS             1187..1486
                     /locus_tag="OO544_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6768284.1"
                     /translation="MGNGNFWQLFLGKEPYSKKSDFVKAWLLWWAITILVLFILSLTT
                     ILNLRSIGIGLSFGFCQILGRYTMSLGWSKYWGVLGVFPVTNIVLFLILIFFKKR"
     gene            complement(1533..2420)
                     /gene="rarD"
                     /locus_tag="OO544_002725"
     CDS             complement(1533..2420)
                     /gene="rarD"
                     /locus_tag="OO544_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HCR6768285.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFAFI
                     WVALAIFVMDAIYTQRRKS"
     gene            complement(2472..2939)
                     /locus_tag="OO544_002726"
     CDS             complement(2472..2939)
                     /locus_tag="OO544_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HCR6768286.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            3104..3973
                     /gene="pldA"
                     /locus_tag="OO544_002727"
     CDS             3104..3973
                     /gene="pldA"
                     /locus_tag="OO544_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HCR6768287.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYCFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            4106..5935
                     /gene="recQ"
                     /locus_tag="OO544_002728"
     CDS             4106..5935
                     /gene="recQ"
                     /locus_tag="OO544_002728"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HCR6768288.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            5999..6616
                     /gene="rhtC"
                     /locus_tag="OO544_002729"
     CDS             5999..6616
                     /gene="rhtC"
                     /locus_tag="OO544_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HCR6768289.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLMVGGGLYLCWMGYQMLRGALKKEAVSAPA
                     PQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTARWGIFALIIVETL
                     AWFIVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(6678..7298)
                     /gene="rhtB"
                     /locus_tag="OO544_002730"
     CDS             complement(6678..7298)
                     /gene="rhtB"
                     /locus_tag="OO544_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HCR6768290.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            7409..8431
                     /gene="pldB"
                     /locus_tag="OO544_002731"
     CDS             7409..8431
                     /gene="pldB"
                     /locus_tag="OO544_002731"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HCR6768291.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            8439..9239
                     /gene="yigL"
                     /locus_tag="OO544_002732"
     CDS             8439..9239
                     /gene="yigL"
                     /locus_tag="OO544_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HCR6768292.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNLDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            9315..10214
                     /gene="bioP"
                     /locus_tag="OO544_002733"
     CDS             9315..10214
                     /gene="bioP"
                     /locus_tag="OO544_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HCR6768293.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(10102..11055)
                     /gene="metR"
                     /locus_tag="OO544_002734"
     CDS             complement(10102..11055)
                     /gene="metR"
                     /locus_tag="OO544_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HCR6768294.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     ITEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            11291..13552
                     /gene="metE"
                     /locus_tag="OO544_002735"
     CDS             11291..13552
                     /gene="metE"
                     /locus_tag="OO544_002735"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HCR6768295.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVAEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIRQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(13837..14082)
                     /locus_tag="OO544_002736"
     CDS             complement(13837..14082)
                     /locus_tag="OO544_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCR6768296.1"
                     /translation="MITETEINVLKIMAEKDINWNWMNLDRTLSIRQIPGFGNVVNIV
                     NKLANEGLVIIEDSGHKSMPHYHVTEKGYNFVKSENK"
BASE COUNT         3447 a         3424 c         3689 g         3599 t
ORIGIN      
        1 acttgaggca ctaaaagcaa cattaataga acctgggcca gggaaatggt atttaataag
       61 tctgtaagga tcaacttttg ctgagatatc gcttaggccg agtgagagaa agtcttgttc
      121 taccatatca ccatattgca tatcatcagc agaaaaatca tcgaataaac taatagtttc
      181 gaaaagtgta cagggaatat taattggcgg aagtaatatg ttattcattt gcatttgcct
      241 tggttatatt taagctcaag atcaattcga gaacaaatgc tagggtaaaa aaatatttaa
      301 aaaagaagta cacttcacat tttttcatta catctctgaa gagaacctcg ttaatgattg
      361 gtcttgctca ttgttaataa caaaatgttg aatttaccga ttaggaaact ttttcctacg
      421 cggattgcct ccagctatgg catgaacttt gagtttatgc cagaactgaa gtggagcttt
      481 ggctaccctg gcgcgattat ctttatgatc cttgcgggcc tgacaccgta tctgtacttt
      541 aagcgtaaga actggctgta actatacgat gtcgctgtga gtaagttagt gtgctgacag
      601 cgacatttta gccgagggaa tcgcagataa cataattgtt gtgaataata tccatgccac
      661 gttgagttgg acgcttaatg actcacatct caccccttca attgctgcag agataataga
      721 aaaaagtgtc aaacccaatg aaatcaatac aaagtagatt gccttacatc cagtatctct
      781 tattcttttt gctccaatat tgatatttac ataggtatta ttaataacca gaataaaact
      841 caaagccagg ttgatatcat tcaaagttat taatattgac catgatgtta atgtatagat
      901 tatgaactga aagcgtgata aacgctcaag cctggatggg tagaatatat ttctgatgga
      961 tgatttgaac atttttacat tgttttatat atataattgt tttgaatata tattacctga
     1021 attatgtggt ttgcatatat atatgcaata tagcagtctt tgcattgata actatacagt
     1081 agtaatttag agtgtgtaag tttttattga tggggtatat tttaattaaa tataattgaa
     1141 atgtattttt attctataaa caatatcttg ggtgaatgga ttctatatgg gaaatggtaa
     1201 cttttggcag ttgttcttgg gtaaagaacc ttacagcaaa aaatctgatt ttgttaaggc
     1261 atggttattg tggtgggcta ttaccatcct tgttctattt attttaagcc tgactacgat
     1321 attaaattta agaagtatag gtatagggtt aagttttggg ttttgtcaaa tccttggtag
     1381 atatacgatg tctctaggat ggagtaagta ctggggagtt ttgggggtat ttcctgtaac
     1441 aaatattgtt ctttttctta tcttaatttt tttcaaaaaa agataaataa gtcagtgggt
     1501 aggctcttta aacggcctac ccatgttaat tcttaacttt ttctacgctg cgtataaatc
     1561 gcatccatca caaaaattgc cagcgccacc caaataaagg cgaaagtcac catcttatcg
     1621 gcacccggtt tttcaccata aaacgtcaca gccagcagga acatcagcgt cgggccaatg
     1681 tactggaaaa agcctaacgt tgagagacgc aagcgcgtgg cagcggcggt aaaacacaac
     1741 agcggtacgg tagtgacaat accggcggcg atcagcagta aattcagcga catcgggttt
     1801 tgccccatat ggctggttga gctgtcggca atagcaaaca ggtaaattgc cgccacgggc
     1861 agcagccaca tggtttcgat taacatgccg gtttgcgctt caacggcaat cttcttgcgt
     1921 accagaccgt agaaggcaaa actaaatgcc agtcccagcg cgataatagg tagcgaacca
     1981 aaagtccaca gctggactaa cacgccacat atcgccagaa tcaccgccag ccattgcatc
     2041 cggcggaatc gctcgccgag gaaaatcatc cccagcacaa tgttcaccag cgggttaata
     2101 aagtaaccaa ggctcgcttc cagcatatgg tgattgttca ccgcccagat aaacagtagc
     2161 cagttgccac caatcagcac ggcagagact gccagcataa aaattttctg tggcgtctga
     2221 atcagcgttt ttaaatagga ccactggcgg caaatgctca tcagcaccac cataaagaaa
     2281 aacgaccaga tcacgcgatg cgtcaggatt tcatcggcgg gcacgtagta aatcaacttg
     2341 aagtacgctg gcgctatacc ccaaataaaa taagcggcaa gagcgagtaa tacgccctgc
     2401 cgcgtttgtt ttgcatccat cgggaatact catttttaat tggtaacagc agtttacctg
     2461 cttttatgtc ttcaacctac catataggtg gcggtggcac tggcaatata aagctgttct
     2521 tcattgtgta attcaacgcg ggcgacggcg actttattgc ctgcacgcaa caggctactg
     2581 gtagcagtaa aacgctcgcc cctgcctggg cgcagataat caacgcgaag atcaatggtc
     2641 cccatccgcg atagccgctg gcgtagttca tcttcactga tggtttcgtg gcgggttaag
     2701 gtacttccca cgcacaccag accggcggcg acatccagcg ccgacgcaat gaccccgccg
     2761 tgcaaaatgc tttgcgccca gttgcccacc atcattggct gatttttaaa ggccagctgt
     2821 gcgaactctt tttcgtaacg ctccagttcc atccccaatg cgcggttaaa tggcatgtga
     2881 taaacaaaca tctcacccac taatttcagg gcttgttcag cggtcagtac ggcagacata
     2941 tcatccttac acttcattgg ttaatgaaat gttgatttta tgcttctttg ttgttggttt
     3001 ctactttagg aagggataac taacggctat ggagttaagt atgtaaaata gcccgcagaa
     3061 aaatattcac cttatcaata attcgttacg gagaacacga ccgatgcgga ctctgcaggg
     3121 ctggttgttg ccggtgttta tgttgcctat ggcagtatat gcacaagagg caacggtgaa
     3181 agaggtgcat gacgcgccag cggtgcgtgg cagtattatc gccaatatgc tgcaggagca
     3241 tgacaatccg ttcacgctct atccttatga caccaactac ctcatttaca cccaaaccag
     3301 cgatctgaat aaagaagcga ttgccagtta cgactgggcg gaaaatgcgc gtaaggatga
     3361 agtaaagttt cagttgagcc tggcatttcc gctgtggcgt gggattttag gcccgaactc
     3421 ggtgttgggt gcgtcttata cgcaaaaatc ctggtggcaa ctgtccaata gcgaagagtc
     3481 ttcaccgttt cgtgaaacca actacgaacc gcaattgttc ctcggttttg ccaccgatta
     3541 ctgttttgca ggttggacgc tgcgcgatgt ggagatgggg tataaccacg actctaacgg
     3601 gcgttccgac ccgacctccc gcagctggaa ccgcctttat actcgcctga tggcagaaaa
     3661 cggtaactgg ctggtagaag tgaagccgtg gtatgtggtg ggtaatactg acgataaccc
     3721 ggatatcacc aaatatatgg gttactacca gcttaaaatc ggctatcacc tcggtgatgc
     3781 ggtgttgagt gcgaaaggac agtacaactg gaataccggt tacggcggcg cggagttagg
     3841 cttaagttac ccgatcacca aacatgtgcg cctttatact caggtttaca gcggctatgg
     3901 cgaatcgctc atcgactata acttcaacca gacccgtgtc ggtgtaggag ttatgctaaa
     3961 cgacttgttt tgatgaacgg ttgagtggtt ggcaaatctg gaacccagca tccaggatta
     4021 ccctctcaga gactaaaagc attgcagttt ctcgcgcagg cgctgaaaat agcgcctgtt
     4081 tttatttcag gcaatcgggg ggaatgtggc gcaggcggaa gtgttgaatc tggagtccgg
     4141 agctaaacag gttttacaag aaacctttgg ctaccaacag tttcgccccg gccaggaaga
     4201 aattatcgac actgtgcttt ccggtcgcga ttgcctggtc gtcatgccca ccggtggcgg
     4261 aaaatccctt tgctatcaaa ttcctgcctt attgctaaac ggccttaccg tggttgtttc
     4321 accgctgatt tcgttgatga aagatcaggt ggatcaactg caagccaacg gcgtggcggc
     4381 ggcgtgcctt aactcgacgc aaacccgcga gcagcaactt gaagtgatga caggctgccg
     4441 caccgggcaa attcgtctgc tttatatcgc cccggaacgc ctgatgctgg ataactttct
     4501 tgagcatctg gcgcactgga atccggtgtt attagccgtt gatgaagcgc actgtatctc
     4561 ccaatggggc cacgatttcc gcccggaata tgccgcgctt ggtcagttac gccagcgttt
     4621 cccgaccctg ccgtttatgg cgctgactgc cacagccgac gacaccacgc gccaggatat
     4681 cgtgcgcttg ctggggctga acgatccgct gattcaaatc agcagttttg accgtccgaa
     4741 tattcgctac atgctgatgg agaaattcaa accgctcgat cagttgatgc gctacgtgca
     4801 ggaacagcgc ggtaagtccg gcattatcta ctgcaacagc cgggcgaaag tagaagacac
     4861 cgctgcgcgc ctgcaaagca agggtattag cgcggcggcc tatcatgccg ggctggaaaa
     4921 taatgtccgc gccgacgtgc aggagaaatt ccagcgcgat gacctgcaaa ttgtggtggc
     4981 gacggtggcg ttcggcatgg gcatcaataa accaaacgtt cgcttcgtgg tccactttga
     5041 tattccgcgc aatatcgaat cctattatca ggaaaccggt cgcgccgggc gtgatggcct
     5101 gcctgcggaa gcgatgctgt tttacgatcc ggctgatatg gcgtggctgc gccgttgtct
     5161 ggaagagaag ccgcaggggc agttgcagga tattgagcgc cacaaactca atgcgatggg
     5221 cgcgtttgcc gaagcgcaaa cttgccgtcg tctggtattg ctgaactatt ttggcgaagg
     5281 gcgtcaggag ccgtgcggga actgcgatat ctgcctcgat ccgccgaaac agtacgacgg
     5341 ttcaaccgat gctcagattg ccctttccac cattggtcgt gtgaatcagc ggtttgggat
     5401 gggttatgtg gtggaagtga ttcgtggtgc taataaccag cgtatccgcg actatggtca
     5461 tgacaaactg aaagtctatg gcatgggccg tgataaaagc catgaacatt gggtgagcgt
     5521 gatccgccag ctgattcacc tcggcctggt gacgcaaaat attgcccagc attctgccct
     5581 acaactgaca gaggccgcgc gcccggtgct gcgcggcgaa tcctctttgc aacttgccgt
     5641 gccgcgtatc gtggcgctca aaccgaaagc gatgcagaaa tcgttcggcg gcaactatga
     5701 tcgtaaactg ttcgccaaat tacgcaaact gcgtaaatcg attgccgatg aaagtaatgt
     5761 cccgccgtac gtggtgttta acgacgcaac cttgattgag atggctgaac agatgccgat
     5821 caccgccagc gaaatgctca gcgttaacgg cgttgggatg cgcaagctgg aacgctttgg
     5881 taaaccgttt atggcgctga ttcgtgcgca tgtcgatggc gacgacgaag agtagtcagc
     5941 agcataaaaa agtgccagga tgaagactcc gtaaacgttt cccccgcgag tcaaatgtat
     6001 gttgatgtta tttctcaccg tcgccatggt gcacattgtg gcgcttatga gccccggtcc
     6061 cgatttcttt tttgtctctc agaccgctgt cagtcgttcc cgtaaagaag cgatgatggg
     6121 cgtgctgggc attacctgcg gtgtaatggt ttgggctggg attgcgttgc ttggcctgca
     6181 tttgattatc gaaaaaatgg cctggctgca tacgcttatg gttggcggtg gcctgtatct
     6241 ctgctggatg ggttaccaga tgctgcgcgg cgcgctgaaa aaagaggcgg tttctgcacc
     6301 tgcgccacag gtcgagctgg cgaaaagtgg acgcagtttc ctgaaaggtt tactgaccaa
     6361 tctcgctaat ccgaaagcga ttatctactt tggctcggtg ttctcattgt ttgtcggtga
     6421 taacgttggc actaccgcgc gctggggcat ttttgcgctg atcattgtcg aaacgctggc
     6481 gtggtttatc gtcgttgcca gcctgtttgc cctgccgcaa atgcgccgtg gttatcaacg
     6541 tctggcgaag tggattgatg gttttgccgg ggcgttattt gccggatttg gcattcattt
     6601 gattatttcg cggtgatgcc agacgcgtct tcagagtaag tcggataagg cgtttacgcc
     6661 gcatccgaca ttatttttca cgcatgcctc gccgatgcta acagcgctcc caccagcata
     6721 aacaacgagc cgaaaatctt attcagcgcc ttcatctgct ttggtccttt aatccataga
     6781 gcaatccgtt gagcaagggt ggcgtaaccg atcatcacaa taatatcgac cacaatagtg
     6841 gtgacgccga gcacgatata ctgcatcagt tgcggctgtt gcggcatgat gaattgcgga
     6901 aatagcgccg ccagaaacac aatacttttg ggattggtga gattcacaaa aactgcgcgc
     6961 tggaacaaat gtcgacgcga ttgagtagag gccagcgatt taaggtcaat tgcaccagcg
     7021 gcgcgccact gctggattcc cagccaaatc aagtaagccg cgcctgccca cttcaacact
     7081 tcaaacgcaa tcactgagcg ggaaaatagc gtccccaacc ccacgccaac cagcacaata
     7141 tgaatcgcca gtccggtctg aagcccagca atagacgcca ccgcgccgcg ataaccgtgg
     7201 ttgagcgagg tggtcatagt gttgattgca ccagagcctg gcgacaggct taaaatgatc
     7261 gatgtcagca ggtaggcaaa ccaccattct aaggtcatga tgaactcccg gtgtgtctat
     7321 ttttgtgcca caatacgcta ctgtcgcagc gttgtgtcag gcacgctaaa aaaaacgatt
     7381 ttacgtggtt taagaggcag attacccgat gtttcagcag caaaaagact gggaaacaag
     7441 agaaaacgcg tttgctgctt ttaccatggg accgctgact gatttctggc gtcagcgtga
     7501 tgaagcagag tttactggtg tggatgacat tccggtgcgc tttgtccgtt ttcgcgcaca
     7561 gcaccatgac cgggtggtag tcatctgccc ggggcgtatt gagagctacg taaaatatgc
     7621 ggaactggcc tatgacctgt tccatttggg gtttgatgtc ttaatcatcg accatcgcgg
     7681 gcagggacgt tccggtcgcc tgttagccga tccgcatctc gggcatgtta atcgctttaa
     7741 tgattatgtt gatgatctgg cggcattctg gcagcaggag gttcagcccg gtccgtggcg
     7801 taaacgctat atactggcac attcgatggg cggtgcgatc tccacattat ttctgcaacg
     7861 ccatccaggt gtatgtgacg ccattgcgct aactgcgcca atgtttggga tcgtgattcg
     7921 tatgccgtca tttatggcac ggcagatcct caactgggcc gaagcgcatc cacgtttccg
     7981 tgatggctat gcaataggca ccgggcgctg gcgcgcgttg ccgtttgcta tcaacgtact
     8041 gacccacagc agacagcgat atcgacgtaa cttacgcttc tatgctgatg acccaacgat
     8101 tcgcgtcggt gggccgacct accattgggt acgcgaaagt attctggctg gcgaacaggt
     8161 gttagccggt gcgggtgatg acgccacgcc aacgcttctc ttgcaggctg aagaggaacg
     8221 cgtggtggat aaccgcatgc atgaccgttt ttgtgaactc cgcaccgccg cgggccatcc
     8281 tgtcgaagga ggacgaccgt tggtaattaa aggtgcttac catgagatcc tttttgaaaa
     8341 ggacgcaatg cgctcagtcg cgctccacgc cattgttgat tttttcaaca ggcataactc
     8401 acccagcgga aaccgctcta cagaggttta aatttcttat gtaccaggtt gttgcgtctg
     8461 atttagatgg cacgttactt tctcccgacc atacgttatc cccttacgcc aaagaaaccc
     8521 tgaagctgct caccgcgcgc ggcatcaact ttgtgtttgc gaccggtcgt caccacgttg
     8581 atgtggggca aattcgcgat aatctggaga tcaagtcgta catgatcacc tccaacggcg
     8641 cgcgcgttca cgatctggat ggcaatctga tttttgctca taacctggat cgcgacattg
     8701 ccagcgatct gtttggcgta gtcaacgaca atctggacat cattactaac gtttatcgcg
     8761 acgacgaatg gtttatgaat cgccatcgcc cggaagagat gcgctttttt aaagaagcgg
     8821 tgttcaaata tgcgctgtat gagcctggat tactggagcc ggaaggcgtc agcaaagtgt
     8881 tcttcacctg cgattcccat gaacaactgc tgccgctgga gcaggcgatt aacgctcgtt
     8941 ggggcgatcg cgtcaacgtt agtttctcta ccttaacctg tctggaagtg atggcgggcg
     9001 gcgtttcaaa aggccatgcg ctggaagcgg tggcgaagaa actgggctac agcctgaagg
     9061 attgtattgc gtttggtgac gggatgaacg atgccgaaat gctgtcgatg gcggggaaag
     9121 gctgcattat gggcagtgcg caccagcgtc tgaaagacct tcatcccgag ctggaagtga
     9181 ttggtactaa tgccgacgac gcggtgccgc attatctgcg taaactctat ttatcgtaat
     9241 cgttctttat ttggtcagtt gtcaacctga tacttcgcta caatggatac tcgttaatca
     9301 aagagttttc cattgtggcg ctacttatca tcaccacgat tctgtgggcc ttctccttta
     9361 gcttttatgg cgagtacctt gcggggcacg tcgatagcta ttttgcggtg ctggtgcgcg
     9421 ttggcctggc ggcactcgtt tttctgccgt ttctgcgtac ccgtggcaat agcctgaaaa
     9481 cggtcggcct gtatatgctg gtgggcgcga tgcagctcgg cgtgatgtat atgctgagtt
     9541 tccgcgctta tctctacctg acggtctccg aattgttgct gttcaccgta ctgacgccgc
     9601 tctacatcac gctgatttat gacatcatga gtaagcgccg tctgcgctgg ggctatgctt
     9661 ttagcgcctt gctggcggtg attggtgccg ggattattcg ctatgatcag gttaccgacc
     9721 atttctggac tggcttgttg ctggtgcaac tctccaatat cacttttgcc atcggcatgg
     9781 tgggttacaa acgcctgatg gaaactcgcc cgatgccgca gcataacgcc tttgcgtggt
     9841 tctatcttgg cgcgttcctg gtggcggtaa ttgcatggtt cttgctggga aatgcgcaga
     9901 aaatgccgca aaccacgttg caatggggca ttctggtgtt tcttggcgtg gtggcttccg
     9961 ggattggcta ctttatgtgg aactacggcg cgacgcaggt ggacgccgga acgctgggca
    10021 ttatgaataa tatgcacgtt ccggcagggc tgctggtaaa cctggctatc tggcaccaac
    10081 agccgcactg gccaacgttc attacaggcg cgctggtgat cctggcctca ctgtgggtgc
    10141 atcgtaagtg ggtcgctccg cgctcttcac aaacggcaga tgatcgcagg cgtgattgcg
    10201 cgctgagcga ataaatgctt ctgtaatcgg ctgacgctgc tcgccatcgc gcacggcggc
    10261 gtacagtcgg ctccacaagc cttcgcccag ggttttggtc accaccagac cttggcgctc
    10321 aaaactctct actacccaat gcggtagcgc ggcaataccc atccgcgcgg caaccatctg
    10381 aatcaacaat aaggtgttat cgacgctttt cagtgacggg ctgacgcctg ccggctgaag
    10441 aaaatgccgc cagacatcca gtcgactacg ctgcaccgga taaatcaaca gcgtctcgct
    10501 ggcgagatct tccggtgtaa ttcgcgtttt cgccgccagt ggatggtcag gtgctaacac
    10561 cagacgcact tcatagtcga acatcggcga ataatgcagg ccactgcgcg gcaggatatc
    10621 ggaggtcatc accagatcca gctcgccctg ttgcaacgca ggctgcgggt caaacgtcac
    10681 gcccgattta aaatccatct ctacctgcgg ccagttctta tggaaatttt ctaacgcggg
    10741 cgtcagccac tgaatacagc tatggcactc aatggcaatg cgcagacgcg tctgctgcgg
    10801 ttcattgcag gcttgcagtg cctggctgat ttgcggcagc acctggtttg ccagttgcaa
    10861 caggatttct ccctgcggtg taaagcgtag cggctggctc ttacgcacaa atagccggaa
    10921 gccaaggcgt tgttccagat cgctaaactg gtgagacagg gcggattgcg tctgatgcaa
    10981 cgtcgccgca gcggctgcga gagagccgca gttccgcaac gcttgtagcg ttttcaggtg
    11041 ttttacttcg atcatgaaag tccttcactt cggcatgaat aatttgcgct tgaggaatat
    11101 acagtaaccg ccaattatgg atgtgtaaac atctggacgg ctaaaatcct tcgtctttta
    11161 aatttatggt gcgttggctg cgtttctcca ccccggtcac ttacttcagt aagctcccgg
    11221 ggatgaataa acttgccgcc ttccctaaat tcaaaatcca taggatttac atataattag
    11281 aggaagaaaa atgacaatat tgaatcacac cctcggtttc cctcgcgttg gcctgcgtcg
    11341 cgagctgaaa aaagcgcaag aaagttattg ggcggggaac tccacgcgtg aagaactgct
    11401 gacagtaggg cgtgaactgc gtgctcgtca ctgggatcaa caaaagcaag cgggtatcga
    11461 cctgctgccg gtgggcgatt ttgcctggta cgatcatgta ctgaccacca gtttgctgct
    11521 gggtaatgtt ccgccacgtc atcagaacaa agacggttcg gtagatatcg acaccctgtt
    11581 ccgtattggt cgtggacgtg cgccgactgg cgaacctgcg gcggcagcgg aaatgaccaa
    11641 atggtttaac accaactatc actacatggt gccggagttc gttaaaggcc aacagttcaa
    11701 actgacctgg acgcagctgc tggaggaagt ggacgaggcg ctggcgctgg gccacaacgt
    11761 taagcctgtg ctgctggggc cggttactta cctgtggctg gggaaagtga aaggtgaaca
    11821 gtttgaccgc cttagcctgc tgaacgacat tctgccggtt tatcagcaag tgctggcaga
    11881 actggcgaaa cgcggcatcg agtgggtaca gattgatgaa cccgcgctgg tactggaact
    11941 accacaggcg tggctggacg catacaaacc cgcttacgac gcgctccagg gacaggtgaa
    12001 actgctgctg accacctatt ttgaaggcgt aacgccaaat ctcgacacga ttactgcgct
    12061 gcctgttcag ggtctgcatg ttgatctcgt acatggtaaa gatgacgttg ctgaactgca
    12121 caagcgcctg ccttctgact ggttgctgtc tgcgggtctg atcaatggtc gtaacgtctg
    12181 gcgcgccgat cttaccgaga aatatgcgca aattaaggac attgtcggca aacgtgattt
    12241 gtgggtggca tcttcctgct cgttgctgca cagccccatc gacctgagcg tggaaacgcg
    12301 tcttgatgca gaagtgaaaa gctggtttgc cttcgcccta caaaaatgcc atgaactagc
    12361 actgctgcgc gatgcgctga acagtggtga cacggcagct ctggcagagt ggagcgcccc
    12421 aattcaggcg cgtcgtcact ctactcgtgt acataatccg gcggtagaaa agcgtctggc
    12481 ggcgatcacc gcccaggaca gccagcgtgc gaatgtctat gaagtgcgtg ctgaagccca
    12541 gcgtgcacgt tttaaactgc cagcgtggcc gaccaccacg attggttcct tcccgcaaac
    12601 cacggaaatt cgtaccctgc gtctggattt caaaaagggc aatctcgacg ccaataacta
    12661 ccgcacgggc attgcggaac atatcaggca ggccattgtt gaacaggaac gtttgggact
    12721 ggatgtgctg gtacatggcg aggccgagcg taatgacatg gtggaatact ttggcgagca
    12781 cctcgacggg tttgtcttta cgcaaaacgg ttgggtacag agctacggtt cccgctgcgt
    12841 gaagccaccg attgttattg gtgacgttag tcgcccggca ccgattaccg tggagtgggc
    12901 gaagtatgcg caatctctga ctgataaacc ggtgaaaggg atgctgactg gcccggtgac
    12961 tattctctgc tggtcgttcc cgcgtgaaga tgtcagccgt gaaaccatcg ccaaacagat
    13021 tgcgctggcg ctgcgtgatg aagtggccga tctggaagcc gctggaattg gcatcatcca
    13081 gattgacgaa ccggctctgc gcgaaggttt accgctgcgt cgtagcgact gggatgcgta
    13141 tctccagtgg ggcgtagagg ccttccgtat caacgccgcc gtggcgaaag atgacacaca
    13201 aatccacact cacatgtgtt attgcgagtt caacgacatc atggattcga ttgcggcact
    13261 ggacgcagac gtcatcacca tcgaaacctc gcgttctgac atggagttgc tggagtcgtt
    13321 cgaagagttt gattatccga atgaaatcgg tccaggcgtc tatgacattc actcgccaaa
    13381 cgtaccgagc gtggaatgga ttgaagcctt gctgaagaaa gcggcaaaac gcattccggc
    13441 agagcgcctg tgggttaacc cggactgtgg cctgaaaacg cgcggctggc cagaaacccg
    13501 cgcggcactg gcgaacatgg tgcaggcggc gcagaatttg cgtcggggat gatgaatatt
    13561 aacgagtcac tgtaaattgc agtacgatga aagaggcaaa actgcctgat actaggccta
    13621 cataatttat gcaatttatt gaatttttgg gttttgttgg ccggacaagg cgttcacgcc
    13681 gcatccggca tgaacaacgc gcactttgcc aataatctga tggcttccat caccggaagc
    13741 cattttgcta tctgcgcccg ttcagataca gaaataaccg aatcattttt acgcaaaata
    13801 taatcctgag cgatttgttc agtaatcaac gctacgttat ttattctcac ttttcacgaa
    13861 attgtatccc ttttcagtaa cgtggtagtg aggcatagac ttatgtccgc tatcctcaat
    13921 aataaccaaa ccctcgttag ctaatttgtt aacaatgtta acaacattac cgaatcctgg
    13981 aatttgcctt atggatagtg tgcgatcgag attcatccag ttccagttta tatctttctc
    14041 agccataatt ttcagtacgt taatttcagt ttcagttatc atagtcaagt gcatcctggt
    14101 ggtataagtc attaagttct cccttaagtt tataatcttc tagagtagca gtatgcgta
//
LOCUS       DAJTZL010000107        14142 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.107, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000107 DAJTZL010000000
VERSION     DAJTZL010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14142
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.107"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..304)
                     /locus_tag="OO544_002737"
     CDS             complement(<1..304)
                     /locus_tag="OO544_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HCR6768297.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLELSLVSQQ
                     FLSLEFPQVLDKMAYLTIWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVCPPLWR"
     gene            complement(514..1032)
                     /locus_tag="OO544_002738"
     CDS             complement(514..1032)
                     /locus_tag="OO544_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HCR6768298.1"
                     /translation="MPTPCYISITGQTQGNITAGAFTADSVGNIYVQGHEDEMLVQEF
                     LHNVTVPTTPQSGQPAGQRAHKPFIFTVALNKAVPLMYNALASGEMLPTTELHWWRTS
                     VEGKQEHYFTTRLTDSTIVDMKLHMPHCQDPAKREFTQLLEVSLAYRKIEWEHVKSGT
                     SGADDWRAPLEA"
     gene            1729..2229
                     /gene="tssB"
                     /locus_tag="OO544_002739"
     CDS             1729..2229
                     /gene="tssB"
                     /locus_tag="OO544_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HCR6768299.1"
                     /translation="MSKKFEGSVAPRERINISYVPKTDGQTAEVELPLNMLVVGDTGN
                     TQETSPLDERQAVSVNKHNFGAVMAEAAIGLNFTVPATLKGSTTDDELNVALNIKSLD
                     DFSPDSVARQVPEVNKLLELREALTALKGPMGNLPAFRTQLQALLENEESREQLLKEI
                     GLVSNK"
     gene            2264..2488
                     /locus_tag="OO544_002740"
     CDS             2264..2488
                     /locus_tag="OO544_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6768300.1"
                     /translation="MTAHKNISGTYHLSVADILQVVYQVCFSPSVEINQDGVAALITT
                     LDRRISDLLDEIIHFCEFQQSASHWQRVLH"
     gene            2539..4014
                     /gene="tssC"
                     /locus_tag="OO544_002741"
                     /pseudo
     CDS             2539..4014
                     /gene="tssC"
                     /locus_tag="OO544_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501045.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
     gene            4021..4434
                     /gene="tssE"
                     /locus_tag="OO544_002742"
     CDS             4021..4434
                     /gene="tssE"
                     /locus_tag="OO544_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HCR6768301.1"
                     /translation="MIPDRERTTGSLFERMEASSARNRQGGSIHSLRQSIRQNLRNIL
                     NTRSGSCRGAPELGIDEPEGAENFRESMSRAIEQCIERYEPRISHAEVQAVVSSASSP
                     LDMTFHITAWVTFNETHEVLEFDMAPNGSQHYRVD"
     gene            4438..6288
                     /gene="tssF"
                     /locus_tag="OO544_002743"
     CDS             4438..6288
                     /gene="tssF"
                     /locus_tag="OO544_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HCR6768302.1"
                     /translation="MEFEERYFREELDYLRQLSKLLATEKPHLARFLAEKDADPDIER
                     LLEGVAFLTGNLRQKIEDEFPELTHGLIKMLWPNYLRPVPAMTLIEYTPDMDKSSVPV
                     LIPRNEQFTTNAGEIRVDEVLPSDAKKEEPPPCTFTLCRDIWLLPVRLEQIENRSTTR
                     NGVINITFSVAPGTDFRTLDLNKLRFWLGNDDNYTRDQLYLWFCEYLQGADLTVGEQH
                     IRLPEFMLKAVGFEPQDAMQPWPKNVHSGYRILQEYFCYPDAFLFFDLCGCPALPDGL
                     QAEFFTLQLRFSRPLPVDIRLRRDSLRLYCAPAINLFIHHAEAITLDNRRADYPLVPS
                     RHYPQHYDVFSVNSVVSQVQDMFRKKDLGRPVSTQAARQWPAFESFSHQIEYSRKREV
                     VYWHHRTKTSLFHRGFDHTLAFIHADGSYPSDESLLSNEVVSVSLTCTNRELPSQIRS
                     GDITGTTGKNAAVASFRNITRPTQPLWPVIDGSLHWPLLSAMNLNYLSLLDTDALKQV
                     IANFDRHAIHHPQTARLSQQKLDAIERLETRPVDRLFTGIPVRGLASTLYLHPEPFVC
                     EGEMYLLGTVLSHFLSLYASVNSFHMLTVVNTESQETWKWTERIGQHPLI"
     gene            6252..7334
                     /gene="tssG"
                     /locus_tag="OO544_002744"
     CDS             6252..7334
                     /gene="tssG"
                     /locus_tag="OO544_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HCR6768303.1"
                     /translation="MDGKNRAASSYLSPGNPPADKEQNDPLAQVFHNACSYNFFAMAE
                     LLHRLAKGEKGTPELSLRDDPAQETLRFSADASLAFPCSDISALKRDTSGAFRMTTTF
                     MGLQGSQSPLPGYYLAHLAWKAVHEQSPVGDFLDMFSHRLTQFVWHIWRKYRYHISFR
                     NGGVDAFSQRMYSLVGLGHRQLRDKLAINHSKMLAYSGILANPGRSPEIICGLVSHCF
                     DLSEVTLQNWQRRKVDIEPDQQNSLGSYSLKNGEKLAGRSVLGNFVLGTRVPDLSGKF
                     QLSITSLTRKQFLSFLPSGENFLPLTMFVSFILRDQLAWDLHLGLAPEQVGAMRLGDN
                     KSALLGWTSFLGTPEERPSVTIRVRS"
     gene            7359..8639
                     /gene="tagH"
                     /locus_tag="OO544_002745"
     CDS             7359..8639
                     /gene="tagH"
                     /locus_tag="OO544_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
                     /protein_id="HCR6768304.1"
                     /translation="MPEEKLQTLSLQVINGSELESGRAARCLFTQQGNVGHGPECHWS
                     VQDRQQSIPAQAFTVILHDGTFCLRPQTAQLWLNQAKVTATSDLIQLRQGDEIQIGRL
                     MVRVHLNRGDIPHYDEEMATPETIVTNRDMLTDTLLSTEGAPHYPGMTHRHQLADTVV
                     NGFSADPLQALQSESLITTGDPLSGIAAVRPSAPLSDPASNGGINTPFMDLPPIYASP
                     GDRNDDVSAAEMAQRHVAVTPLLRGLGGSLTMSNSDDADDFLEEAGRTLQAAIKGLLD
                     LQQQRNSLSDKHLRPLEDNPLRLNMDYATALDVMFAEGKSPVHLAAPAAVSESLRNVR
                     HHEEANRAAIVESLRVLLDAFSPQNLLRRFVQYRRSHELRQPLDDAGAWQMYSHYYEE
                     LASDRQQGFEMLFNEVYAQVYDRVLREKQREPEA"
     gene            8636..9160
                     /gene="tssJ"
                     /locus_tag="OO544_002746"
     CDS             8636..9160
                     /gene="tssJ"
                     /locus_tag="OO544_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HCR6768305.1"
                     /translation="MNRKAFLACVLMCVILTGCETAKKISEVIKNPDIQVGSLKEQPS
                     EITVTLLTEPDTNTNAEGESAAVDVQLVYLTDDSKLLAADYDQIASTPLPDVLGKNYI
                     DHQDFNLLPDTIKTLPPVKLDEKTQFIGVVAYFSDDQATEWKQIETVEGIGHHYRLLV
                     HVRQSSIEMKKEDE"
     gene            9163..10492
                     /gene="tssK"
                     /locus_tag="OO544_002747"
                     /pseudo
     CDS             9163..10492
                     /gene="tssK"
                     /locus_tag="OO544_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308252.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
     gene            10497..11258
                     /gene="icmH"
                     /locus_tag="OO544_002748"
     CDS             10497..11258
                     /gene="icmH"
                     /locus_tag="OO544_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HCR6768306.1"
                     /translation="MDEGSLSLPPFTGHDEKSQRNYHLALRGNSLNPMIDAATPLLGM
                     VMRLSTMNSQTMPEHLFAQVVTDVQAVEQLLQEQGYEPGVIISFRYILCTFIDEAALG
                     NGWSNKNEWIKQSLLVHFHNEAWGGEKVFILLERLIREPKRYQDLLEFLWICFSLGFR
                     GRYKVAAQDQGEFEQIYRRLYHVLHKLRGDAPFPLLHQDKKTQGGRYQLISRLTVKHI
                     FCGGVVVLALFYLFYLLRLDSQTQDILHQLNKLLR"
     gene            11267..14050
                     /gene="tssH"
                     /locus_tag="OO544_002749"
                     /pseudo
     CDS             11267..14050
                     /gene="tssH"
                     /locus_tag="OO544_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308250.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
BASE COUNT         3382 a         3813 c         3778 g         3169 t
ORIGIN      
        1 tgcgccacag cggtgggcat accttcatgc tgtacagcat ctggtttttg tcgttctccc
       61 ccagttcaaa ccaggtcacc acacctttca cccggcgctg tacgtcatcg ccctgccata
      121 tcgtcaggta ggccattttg tccagtacct gcggaaattc aagggagaga aactgctggc
      181 tgaccagaga gagctcgagg gaaaagagcg aagagagtga ctggttcaga tgaaaggaga
      241 ccaccgcaaa agcatccggt ggcaggccgt ccacttccag cgtgaaacgt aatccggttg
      301 acatagcaac ctccggggtt aaggaaaaac agacagttcg ctgttaaagc ggctgtgaca
      361 gtacagtgtt acgcttgcgt tcaggcacgc taccgccgaa gtttccggca tccgccatct
      421 cccgtgaacg taacccctta ctgacaacct ttctttctac ggcgtaatac aaaaacagca
      481 tccggcctga tagccggatg ctgttggctt aacttatgct tccagtggtg cgcgccagtc
      541 gtcggcaccg gaggtgccag atttgacgtg ttcccattca attttgcggt aggccagtga
      601 aacctcgagc aactgggtga actcgcgttt cgccgggtcc tggcaatgcg gcatatgcag
      661 tttcatatcg acaatcgtcg aatcagtcag gcgggtagtg aaataatgct cctgtttgcc
      721 ctccacagag gtacgccacc agtgaagttc agtggttggc agcatttcgc cggaggccag
      781 tgcgttgtac atcagcggaa ccgctttgtt cagcgccacg gtaaaaatga aaggcttgtg
      841 agcacgctga cccgcaggct gaccggactg aggagtggtc gggacagtaa cgttatgcag
      901 gaattcctgc accagcatct cgtcttcgtg cccctgcaca tagatgttgc cgacagaatc
      961 agcggtaaat gcacctgcag taatatttcc ctgggtctga ccagtgatgg agatataaca
     1021 cggtgttggc atttgtaaac tccttgttaa cgtggttaat tactacttac aatgacctgc
     1081 cgttaatccc agacaggcag atgcagctca cttcacggaa ccgcatcatt tttcagtatt
     1141 ttttacatac aaaccgagct agaatttacg gttgtcgtaa aaactatttt taaaatcatt
     1201 aaatctctta ttcagcaata acttaataat atatatctgg ataaccatac ccaactgttc
     1261 aaagtccgta ttgcctccat aaaatggcca aagctgaatc atgaataaat attgtattta
     1321 tcctttctga attatcattt tgataatttt tgagatattc cttaaaaata cccacttaat
     1381 caatcacaaa acaaatatat aaacaagcaa tcattagatt agcttatttt taatgaaatt
     1441 attatatact tcaataagtt acacaaaaac tctatattta aattattgcc ataatacacc
     1501 tgaagcgaat tgcacaggtt cacacaatct atttaacgct tgcagaatac ccctctttac
     1561 gatcgataaa aagtcctttt cttatttatt ttgaagcgta aaatgcgggg tgatgaagga
     1621 gtggtgattt tattttcacc gccattaggg cggcattgcc gatatttttt tgcggtacaa
     1681 atatgccgtg ttcatggcct gtacgagata acatgacgga tcacttttat gagcaaaaaa
     1741 tttgaaggca gcgtcgcgcc ccgcgagcgt attaatatat cgtatgtccc aaaaacagat
     1801 ggccagacgg ctgaagtgga acttccgctt aacatgctcg ttgtcggcga taccggaaat
     1861 acgcaggaaa catccccact ggatgaacgt caggcagttt ccgttaacaa gcacaatttc
     1921 ggtgcagtga tggctgaagc agccattggc ctcaatttta ctgtccccgc cacgctgaag
     1981 ggaagcacaa cagacgatga actgaatgtg gcgctgaata tcaaatcact ggatgatttt
     2041 tcaccagata gcgtggcccg gcaggttccg gaagtgaata aattgctgga actgcgtgaa
     2101 gcactgactg cactgaaggg gccgatgggt aaccttccgg cattccgtac ccagctccag
     2161 gcattgctgg aaaatgaaga gtcccgcgag cagttgctca aagaaatcgg tctggtgagc
     2221 aataaataac caccactatt tcattaaaca gggttatcca ggcatgacgg cacacaaaaa
     2281 tatctccggg acataccacc tctctgttgc agacattctg caggtggttt accaggtatg
     2341 tttcagcccg tccgttgaaa tcaaccagga tggtgtggct gcgctgatta caacactgga
     2401 tcgccgtatc agtgatctat tggatgagat tatccatttt tgtgagtttc agcagagcgc
     2461 gtcgcactgg cagcgcgtac ttcactgatg gcgaaaagcc ggtttcagaa acacagctaa
     2521 aacagaagga ttttcagtat gtctttacag gaagaggaac tcgtttccag tcatgccggg
     2581 cagcctgagc aggcatcgtc gctgcttgat caaatcatgg cacagacccg tattcagccc
     2641 ggttcggaag gttatgacgt tgccctccag ggtgtgacag cctttattgc cagcatcctg
     2701 caatccactg catctgccga accggtcaat aaactggccg tggacagcat gattgcagat
     2761 attgatgaac gcatcagccg ccagatggat gtcatcatcc atgcgcctgc ctttcagcag
     2821 gtcgagtcgt tctggcgctc actgaaaacc atggtggatc gcgttgattt ccgtgaaaac
     2881 atcaaggtca acgtgcttca tgtcaccaag caggaacttc tggaagactt tgagtttgcg
     2941 ccagaaatta ttcagtccgg attttataag cacgtttatt cgtccggttt cggacagttt
     3001 ggcggtaaac ccatcgctgc cgtgctgggc gcttatgagt tcaaaaatac cgcgcctgac
     3061 atgaaactgc tgcaatacgt cagtgccgtg ggcgcaatgg cgcacgcacc gttcctgtcc
     3121 tccgtttccc cggagtttat ggggctgaac tcgtggaccg aactgcctaa tatcaaagat
     3181 ctttatgcca tctttgaagg tccggcctac accaaatggc gcgccctgcg tgactcggaa
     3241 gattcgcgct atttagggct tacggcaccc cgcttcctgc ttcgccagcc ctattcacca
     3301 acagataacc ctgttaagaa ctttaactat tacgaagatg tcagccagaa ccacgaagat
     3361 tatctgtggg gtaatacggc ctggatgctg gcatgcaata tcgcagacag ttttgccaaa
     3421 taccgctgat gtccaaacat tatcggtccg caaagcggcg gcgcagtgaa agatctgccg
     3481 atgcatctgt tcgaaacgat ggggcagatt caggccaaaa tcccaaccga ggtgctggtc
     3541 accgatcgcc gcgaatttga actggctgaa gagggtttta tcacccttac catgcgtaaa
     3601 gactctgata atgcagcctt tttctctgca aactcggtac aaaaaccgaa gcactttccg
     3661 ggaaaagatg cagaaaccaa ctataagctg ggtacgcagc ttccttatct cttcatcatc
     3721 aatcggttag cgcactacat caaggtgttg cagcgtgaac aactggggtc atggaaagaa
     3781 aggagcgact tagaaagaga gcttaatacc tggatccgtc agtatgttgc cgaccaggaa
     3841 aacccgcctg ctgacgtgcg cagccgcaaa cctctgcgcg ctgccaaagt tgaggttatg
     3901 gatgtagaag gcgaacccgg ctggtaccag gttgcgctaa gcgtgagacc tcattttaaa
     3961 tttatggggg caaattttga gctttccctg gttggccggt tagacaggga gtaacacacc
     4021 atgataccgg atcgggaaag aaccacaggt tctctttttg agcgtatgga agcctcttcg
     4081 gccagaaacc ggcagggagg cagtattcat tcgctcaggc aatccattcg ccagaaccta
     4141 cgaaacattc ttaatacccg gtccggtagt tgccggggtg cgcctgagct gggcattgat
     4201 gaaccggaag gggctgaaaa cttccgcgaa tccatgagtc gggccatcga gcaatgtata
     4261 gaacgctatg aaccccggat ctcacacgct gaagtgcagg cagtggtttc atccgcctcc
     4321 agcccgctgg atatgacgtt tcatattaca gcctgggtga catttaacga aacgcatgag
     4381 gtgcttgaat ttgatatggc accgaatggc agccagcatt accgggtgga ctgacaaatg
     4441 gaatttgaag aacgctattt ccgggaagaa ctcgattacc tgcgccagct tagcaagctg
     4501 ctggcaacgg aaaaacccca tctggcccgc ttcctggccg aaaaagatgc agatccggat
     4561 attgaacgcc tgctggaagg ggtggctttt cttaccggca atctccgcca gaaaattgag
     4621 gatgaattcc cagaactgac gcacgggctt attaagatgc tatggcctaa ttacctgcgt
     4681 ccggttccgg caatgaccct tattgaatat acgccggata tggataagtc ttctgtaccg
     4741 gtgttaatcc cccgtaatga gcagtttaca accaacgccg gggaaatcag agttgatgaa
     4801 gtgctgccct ctgatgctaa aaaggaggag ccgcctccct gtaccttcac cctctgccgg
     4861 gatatctggc tgctgcccgt tcgcctggag cagattgaaa accgcagtac gacccgtaat
     4921 ggtgttatca acatcacctt ttcggtcgca ccgggaacgg acttccgcac gctggatctg
     4981 aacaaacttc gcttctggct cggcaatgac gacaactata cccgtgacca gctttattta
     5041 tggttctgcg aatacttgca gggtgccgac ctgactgtgg gtgaacagca tattcgcctg
     5101 cctgagttta tgctaaaagc tgtcggtttt gagccgcagg atgccatgca gccctggccg
     5161 aaaaacgtcc acagcggcta ccggatcctt caggagtatt tctgttaccc cgatgcgttt
     5221 ctcttttttg atctttgtgg ttgtccggct ttgcctgacg gattgcaggc ggaattcttt
     5281 accctgcaac tgcgtttttc gcgccctttg cccgtggaca tccggctgcg ccgcgattcc
     5341 ctgcgcctgt attgcgcacc tgccattaat ttatttatcc accatgcaga agccatcacg
     5401 ctggacaacc ggcgggcaga ctatccgctg gttcccagcc gccattaccc acaacattac
     5461 gatgtatttt ccgttaacag tgtggtgagc caggtccagg atatgttcag gaaaaaagat
     5521 ctggggcgtc ctgtttcgac gcaggccgcg cgccagtggc cagcctttga aagtttcagc
     5581 catcagatag aatacagccg gaagcgggaa gtggtgtact ggcatcaccg gaccaaaaca
     5641 tccctgttcc atcgcggctt tgatcatacc cttgccttta tacatgctga tggcagttat
     5701 ccgtcagacg aatctctgct cagtaatgaa gtggtttcgg tatcgctgac ctgtaccaac
     5761 cgtgagcttc cgtcacaaat tcgttccggc gatatcaccg gcacaaccgg aaaaaatgcg
     5821 gctgttgctt catttcgcaa cattacccgc ccgacgcaac cactctggcc ggtcattgat
     5881 ggcagcctgc actggcccct actctccgcc atgaacctga attatctgtc attactggat
     5941 acggacgcgc tgaagcaggt catcgccaac tttgatcgcc acgcaatcca tcatccgcag
     6001 acggcacggc tgtcacaaca aaagctggat gccattgagc gtctggagac ccgccccgtt
     6061 gatcgcctgt ttacgggtat tcccgtccgg gggctggctt ccacgctgta tctgcacccg
     6121 gagccgtttg tctgtgaagg ggaaatgtat ctgctcggta cggtgctttc gcattttctg
     6181 tcgctgtacg ccagcgttaa ctcattccac atgctgaccg ttgtgaacac agaaagccag
     6241 gagacatgga aatggacgga aagaatcggg cagcatcctc ttatctgagt ccgggaaatc
     6301 ccccggcaga taaagagcag aatgatccgc tggcacaggt tttccataat gcctgctctt
     6361 acaatttttt tgcgatggcg gagctgttgc accgcctggc aaagggtgaa aaggggacgc
     6421 cagaattatc cctgcgcgac gatccggcac aggaaaccct gcgtttcagc gccgatgcca
     6481 gccttgcctt cccgtgcagt gatatcagtg cgctgaaacg ggatacgtca ggggcattca
     6541 ggatgaccac cacctttatg gggttacagg ggagccagtc gcctctcccc ggttattacc
     6601 tcgctcacct ggcctggaaa gcggtacatg aacaaagtcc ggtaggtgat tttctggata
     6661 tgttcagcca ccgcctgacc cagtttgtct ggcatatctg gcgtaagtac cgctatcaca
     6721 tcagttttcg taacggcggt gtggatgcgt tctcgcagcg catgtactcc cttgtcgggc
     6781 tgggccaccg ccagcttcgc gataagctgg cgattaatca cagcaaaatg ctggcgtatt
     6841 ccgggatcct ggcaaatccg ggacgatccc cggaaattat ctgtggcctg gtgtcccact
     6901 gctttgattt gtcagaagtg acactgcaaa actggcaacg tcgcaaggtg gatattgaac
     6961 cggatcagca aaatagtctg gggagctatt ctcttaaaaa tggcgaaaaa ctggcgggaa
     7021 gatcggtact gggcaacttt gttctgggta cacgagtgcc cgatctcagc ggaaaattcc
     7081 agctcagtat taccagcctg acccgaaaac agttcctctc ttttctgccg tccggcgaaa
     7141 acttcctgcc actgacgatg ttcgtgtcct tcatcctgcg cgatcagctt gcctgggact
     7201 tacatctggg gctggcgccg gagcaggtgg gcgcaatgcg cctgggcgat aacaaaagtg
     7261 cgctgctggg ctggaccagc ttcctcggca caccggaaga acgaccctca gtcaccatca
     7321 gggtacggtc gtaaattatg ataatcacgg agcagaatat gccggaagag aaactacaga
     7381 cgctttcatt gcaggtcatt aacggcagtg agctggaaag cgggcgggcg gcgcgctgtc
     7441 tgttcacaca gcagggaaat gtcggccatg gccccgaatg ccactggtcg gtacaggatc
     7501 gtcagcagag cattccggcc caggctttta ccgttatcct gcacgatggc acattttgtc
     7561 tgcgcccgca gacggcacaa ctgtggctga atcaggcaaa agtcacagca acatcagacc
     7621 taatacagtt gcgccagggc gatgagatcc agatcggacg gctgatggtg agggttcatc
     7681 tgaaccgggg agatattccc cattacgatg aggaaatggc cactcccgaa accatcgtta
     7741 ccaatcgcga tatgctcacg gataccctgc tatcaacgga gggtgcgcca cactatccgg
     7801 gaatgactca ccggcaccag cttgcagaca ccgtggtaaa cggtttttct gccgatccac
     7861 tccaggctct tcagtccgaa agcctgatta ccacgggcga tccgctttca ggcattgcgg
     7921 ctgtccggcc atcggcaccg ctgtccgatc cggcaagtaa tggggggatc aatactccgt
     7981 ttatggatct gccgcccatt tatgccagcc ccggcgatcg taatgacgac gtctctgcgg
     8041 cagaaatggc acaacgccac gttgcggtca cccccttact gcgcggtctt ggcggctcgc
     8101 ttaccatgag caattccgac gatgcggatg attttctgga ggaggccgga cgaacgttac
     8161 aggccgcaat aaaaggtctg ctcgatttgc agcagcagcg taacagcctc tcagacaaac
     8221 atttgcgccc gctggaagat aacccgctgc gcctgaacat ggattacgcc accgcgctcg
     8281 acgtaatgtt tgccgaaggt aaaagcccgg tacatctggc ggctcccgcc gccgtcagtg
     8341 aaagcctgcg caatgtccgc caccacgaag aagctaaccg ggcagcgatt gtggagtcgc
     8401 ttcgtgtcct gctggatgct ttctcaccac aaaatctgct gcgccgcttt gtgcagtacc
     8461 gccgcagcca tgaactgcgc cagccgctgg atgatgccgg agcatggcaa atgtacagcc
     8521 attattacga agaactggcc tccgatcgcc agcaggggtt tgagatgctg tttaacgagg
     8581 tctacgccca ggtctatgac cgggtgcttc gtgaaaaaca gcgggagccg gaagcatgaa
     8641 caggaaggcg tttctggcct gcgtgctgat gtgcgtcata ttaaccggct gcgaaacggc
     8701 gaaaaaaatc agtgaggtga tcaaaaaccc ggatatccag gtcgggagcc tgaaggagca
     8761 accttcggaa ataaccgtca ccctgctgac cgaaccggac accaatacca atgcggaagg
     8821 tgaatcagcc gccgtggatg tgcaactggt gtacctgact gacgactcaa aattgctggc
     8881 agccgactat gaccagatag ccagcacgcc gctaccggat gtgctgggta aaaactatat
     8941 cgaccaccag gatttcaacc tgttgccgga caccatcaaa accctgccgc cggtaaagct
     9001 ggatgagaaa acgcagttta ttggtgtcgt ggcctatttc tccgatgacc aggccactga
     9061 atggaaacaa atcgagacgg tggaaggaat cggccaccat taccgcctgc tggtgcatgt
     9121 acgtcaaagc agcatcgaaa tgaaaaaaga ggatgaataa ttatggcaac cacccgcaac
     9181 aaggtgatgt ggcaggaagg gatgctgatg cgtccacacc atttccagca gcagcagcgt
     9241 tacaacgact acctggataa ccagcgtttc cgggccatga atgatttatc ctggggattt
     9301 accgaactca ccctcaacaa tgaactgctg gcgcagggta agatcatgat tgacagcgcg
     9361 tcaggcacac tgcccgacgg caccgtcttt tctatccccg accaggacgc actgcccgat
     9421 ccgctgcacc cgcaaaattt tccggacgaa agaagccgca atatctacct cgctctgccg
     9481 gtcgccagtg atgtgagaaa tgaaatcagc gacggacggc gaatcgggcg ttaccggctg
     9541 aattatgccg atgtccggga tttgcattca gaagaaggtg acgcgcgaac attgacgctg
     9601 ggtcaactga cgccgcgcat tatgagcggt gcagaagata tgagcgccta tattacgctc
     9661 ccgctttgcc gtatcagcga tcgccgccga tggctccctg acgttggatg acgattttat
     9721 cccctcctgc cagaatattc aggtcagtaa gaaactgcgt gtttatctca aagaggtaca
     9781 gggggccatt ggcggacggg caagcgatct ggcaaaccgt attggctctc cggcgcagag
     9841 cggcatcgcg gatgtggcgg aatttatgat gttgcagtta cttaaccgta accagacccg
     9901 gtttacccat cacgcccgtc gatcccagct ccacccggaa gatttctacc ttgatcttgc
     9961 cgggttgctg ggtgaactga tgacctttac cgagccgtcg cgcctgccct gcccgcttga
    10021 tgtgtatgat catcatgacc tgaccaaaat atttaaaacg ctgttaccgg aagtcaaacg
    10081 ggcgctgcat accgtactgt cgccaagagc cgtcaatctg ccgctgcatc tgcgtgacgg
    10141 tatctggcag gccgatatcc atgacacaga actgctgcaa tctgccacct ttgtgctggc
    10201 cgtggcggca aacatgccgg tcgatcagat ccagcgccag tttatccagc agtcgaaaat
    10261 ttcctcgccg gaaaaaatcc gcaatatggt cagtgtgcag atccccggta ttccattgcg
    10321 tgcccttatg gtggctcccc gccagcttcc ttaccattcc gggttcagct atttcgaact
    10381 cgacaagagt ggccaggcct ggacagaaat ggctgccgcc ggggccgttg cactgcatgt
    10441 ttccggcagt ttcccggatc tgaacatgca actgtgggcg ataagagggt aagacgatgg
    10501 atgaggggtc actgtctctg ccaccattta ccggacacga cgaaaaaagc cagcgcaact
    10561 accatctcgc gcttcgtggc aacagcctga atccgatgat cgacgcggcc acgccgctac
    10621 tcggtatggt aatgcgcctt tcaaccatga acagccagac gatgccggag catctttttg
    10681 ctcaggtggt gacagatgtt caggccgtgg agcaactgct tcaggagcag ggctacgaac
    10741 cgggcgtgat tatctcgttt cgctacatcc tctgcacgtt tattgatgaa gcggcgctcg
    10801 gtaacggctg gtcaaacaag aacgagtgga tcaagcagtc attgctggtc catttccaca
    10861 acgaagcctg gggtggcgag aaagttttca tcctgctgga gcgcctgatc cgtgaaccca
    10921 aacgttacca ggatctgctg gaatttctct ggatctgttt ttctctggga tttcgcgggc
    10981 gctacaaagt ggcagcacag gatcaggggg aatttgagca gatctaccgc cgtctgtacc
    11041 acgttctgca taaactgcgc ggcgatgccc ctttcccgct gttacaccag gacaaaaaaa
    11101 cccagggcgg gcgttatcag ctcatcagtc gcctgactgt taaacatatt ttttgtggtg
    11161 gcgttgtcgt gctggcgttg ttttacctgt tctatctgtt gcggctggac agccagacgc
    11221 aggacatcct gcaccagcta aacaaattac ttcgataagg acgtttatga tccagattga
    11281 tcttcccacg ctggtaaaac ggctgaacct gttctcccgc caggcgctgg agatggccgc
    11341 ctctgaatgt atgagtcagc aggcagcgga aattaccgtc agccatgtgc tcattcagat
    11401 gctcgccatg ccacgcagtg acctgcgggt tattacccgc cagggcgata ttggcatgga
    11461 agagttgcgc caggcgctga cggtagagaa ctacacaacc gcccgttctg cggacagcta
    11521 cccggcgttt tccccgatgc tggttgagtg gcttaaagag ggctagctgc tggcgtcggc
    11581 tgagatgcag cacagcgaac tgcgcggcgg cgtgttgctg ctggccctgc tgcattcgcc
    11641 gctgcgttat ataccgcctg ctgccgcccg gctgttgacc ggcattaacc gtgaccgtct
    11701 gcaacaggac tttgtgcagt ggacacagga gtcggcggaa tcagtcgtgc cggatgcaga
    11761 cggtaaaggc gcaggcacaa tgacggacgc ctctgacacc ctgcttgccc gctatgccaa
    11821 aaacatgacc gcagacgccc gtaacggcag gcttgacccg gtactgtgcc gcgaccacga
    11881 aatcgacctg atgatcgaca ttctctgccg ccgccgtaaa aacaacccgg tggtggtggg
    11941 cgaagcgggc gtgggcaaaa gcgcactgat tgaagggctg gcgctgcgca tcgtggcagg
    12001 ccaggtgccg gacaagctga aaaacaccga tatcatgacc cttgacttgg gcgcattgca
    12061 ggccggggcg tcggtgaagg gtgaattcga aaaacgtttc aaagggctga tggcggaggt
    12121 catttcctcc ccggtgccgg tcattctgtt tatcgacgaa gcacataccc tgattggcgc
    12181 gggcaaccag cagggcgggc tggatatctc caacctgctc aaaccggcgc tggcgcgcgg
    12241 cgagctgaaa accatcgccg ccaccacctg gagcgagtac aaaaaatact tcgaaaaaga
    12301 tgccgccctg tcgcgccgct tccagttggt gaaggtcagc gaacccaacg ctgccgaagc
    12361 caccattatt ctgcgcggtc tgtcggcggt ctatgaacag tctcacggcg tgctgattga
    12421 tgatgacgcc ttgcaggccg ctgcgacatt aagcgagcgt tatctctccg ggcgtcagtt
    12481 accggacaaa gcgattgatg tgctggatac cgcctgcgcc cgtgtggcca tcaacctgtc
    12541 gtcgccgccg aagcaaatct cggcgctgac cactctgagc caccagcagg aggcggaaat
    12601 tcgccagctt gagcgcgagc ttcgcatcgg actgcgtacc gacacatcac ggatgaccga
    12661 ggtgctggtg cagtatgatg aaacgctgac ggcgctggat gaactggaag cggcctggca
    12721 ccagcagcag acgctggtcc gggagattat cgcgctgcgc cagcagttac tgggcgtggc
    12781 agaggacgat gcggcgccgt tgccggacgc agataccgtg gaggatacgc agccagagtc
    12841 agagtcagag tcagagtcag agtcagagca ggataatacc ggtgccgaac cggctgatga
    12901 ggccgacaga gaacaaccgg aagagaccgc tgaaacagtt tccccggtac agcggctggc
    12961 ccagctcact gccgaactgg acgccctgca taacgaccgg ttgctggtct ccccgcacgt
    13021 cgataaaaaa cagattgcgg cggtgattgc cgaatggacc ggcgtgccgc tcaaccgcct
    13081 gtcgcagaat gaaatgtcgg tcatcaccga cctgacgaaa tggctgggtg acaccatcaa
    13141 aggccaggac ctggcgattg ccagcctgca taagcatcta ctgaccgcac gcgccgacct
    13201 gcgtcgtccg ggacgcccgc tcggtgcgtt cctgctggct ggccccagcg gtgtgggtaa
    13261 aaccgaaacc gtcctgcaac tggcagagct gctctacggc ggtcgccagt acctgaccac
    13321 catcaatatg tccgaattcc aggagaaaca caccgtctcg cggctgattg gttcgcctcc
    13381 gggctacgtt ggctacggtg aaggcggcgt actgaccgaa gcgattcgcc agaaacccta
    13441 ctcggtagta ctgctcgatg aagtggaaaa agcgcacccg gatgtgctca acctgttcta
    13501 ccaggcgttc gataagggcg aaatggcaga cggtgaaggc cgcctgattg actgcaaaaa
    13561 tatcgtcttc ttcctgacgt ccaacctcgg ctaccaggta atagtcgagc atgccgatga
    13621 cccggaaacc atgcaggaag tacagtatcc ggtgctggcc gacttcttca aacctgccct
    13681 gctggcgcgt atggaagtgg tgccgtatct gccgctgtcg aaagagacgc tcgccaccat
    13741 tatcgccgga aaactggccc gtctggataa cgtgctgcgc agtcgctttg gtgtagaagt
    13801 ggtcattgaa ccggaagtga cggacgaaat catgagccgc gtcacccgcg cggaaaacgg
    13861 cgcgaggatg ctggaatcgg tcatcgatgg cgacatgcta ccgccgctct cgctgctgct
    13921 gttgcagaaa atggcggcta acacggcgat tgcccggatt cggttgtcgg cagtggacgg
    13981 cgcatttacg gcagacgtgg aagatgctca gaacgacgag tccgtcacaa aggatgaaac
    14041 ggttttatga gcaggttgcg cgttttccgg cagacaggat ggtttatcgc gggcctgatg
    14101 acgggcttac ccgccactgc cgcgcccgca gaagcatcgt ca
//
LOCUS       DAJTZL010000108        14107 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.108, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000108 DAJTZL010000000
VERSION     DAJTZL010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14107
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.108"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(13..1371)
                     /gene="hprS"
                     /locus_tag="OO544_002750"
     CDS             complement(13..1371)
                     /gene="hprS"
                     /locus_tag="OO544_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HCR6768307.1"
                     /translation="MKRLSITVRLTLLFILLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIVLCSVLSPLLIRTGLREIKKLSGVTEALNYNDSREPVEVSALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDTNGYLNIDVASPGTK
                     IHEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLNKHNVFRIMLPQ
                     RN"
     gene            complement(1371..2042)
                     /gene="hprR"
                     /locus_tag="OO544_002751"
     CDS             complement(1371..2042)
                     /gene="hprR"
                     /locus_tag="OO544_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HCR6768308.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLRMDSVSQSVSRNNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            2175..2588
                     /gene="hiuH"
                     /locus_tag="OO544_002752"
     CDS             2175..2588
                     /gene="hiuH"
                     /locus_tag="OO544_002752"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HCR6768309.1"
                     /translation="MLKRYLVLSVVTAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            2697..3701
                     /gene="msrP"
                     /locus_tag="OO544_002753"
     CDS             2697..3701
                     /gene="msrP"
                     /locus_tag="OO544_002753"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HCR6768310.1"
                     /translation="MKKNQFLKESDVTAESVFFMKRRQVLKALGISAAALSLPHAAHA
                     DLLSWFKGNDRPPAPAGKPLEFSKPTAWQNNLPLTPVDKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYADQVASLYRGLDLRENF
                     "
     gene            3702..4337
                     /gene="msrQ"
                     /locus_tag="OO544_002754"
     CDS             3702..4337
                     /gene="msrQ"
                     /locus_tag="OO544_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HCR6768311.1"
                     /translation="MRLTAKQVTWLKVSLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLATLLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQAMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLRSLFKRLRKQVHNKLSV"
     gene            4594..5244
                     /gene="zinT"
                     /locus_tag="OO544_002755"
     CDS             4594..5244
                     /gene="zinT"
                     /locus_tag="OO544_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HCR6768312.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDANVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKIKTFAEIKDYYHKGYA
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQFLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            5587..6117
                     /locus_tag="OO544_002756"
                     /pseudo
     CDS             5587..6117
                     /locus_tag="OO544_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416483.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
     gene            complement(6687..6776)
                     /locus_tag="OO544_002757"
     tRNA            complement(6687..6776)
                     /locus_tag="OO544_002757"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6740..6742),aa:Ser,seq:cga)
     gene            6870..7667
                     /gene="mtfA"
                     /locus_tag="OO544_002758"
     CDS             6870..7667
                     /gene="mtfA"
                     /locus_tag="OO544_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HCR6768313.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTGLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELDSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFIPLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            7768..7843
                     /locus_tag="OO544_002759"
     tRNA            7768..7843
                     /locus_tag="OO544_002759"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7801..7803,aa:Asn,seq:gtt)
     gene            8157..14107
                     /gene="yeeJ"
                     /locus_tag="OO544_002760"
                     /pseudo
     CDS             8157..14107
                     /gene="yeeJ"
                     /locus_tag="OO544_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin YeeJ"
BASE COUNT         3991 a         3353 c         3170 g         3593 t
ORIGIN      
        1 tgaagcccta tgttaatttc tttgcggtaa cataatccgg aacacattat gcttattgag
       61 atagtgatac gtagcacttc ccccatgtaa ttcggcaatc gctttgacta aagaaagacc
      121 aagtccctga cctacggaat gacgcgaatt atctccccgc caaaatctac ggaagagttt
      181 ttcaggctca tgaattttcg ttccaggact ggcgacatca atattaagat agccgttggt
      241 atcaagaaaa ctggttatat gaatacgcga tttttctggt gaatatctaa tggcattaac
      301 aataagattc gataacattc gttgcagtaa aattttatcc gcaaagattt gctgattgca
      361 ctcgacctta aagcaaatct ctttctcgtc tgaaaggtat tcaagataat ccaacaaatt
      421 ttcgacttcc ttattgagag aaagcgagtc cagtttcacc aaaacattgt ttttatctgc
      481 ccgggcaaga aacagtatgt tctctgttaa ccgcgaaata ttttccagct cttcaatgtt
      541 accggcaatt gttttttgat attcagcgat acttctggtt tgactgagcg taacctgatt
      601 ctgacccagt aatgcattaa tgggcgttct aagttcatga gcgagatcgt cagcaaattg
      661 acttagacgc tcaaaatctt tgactaaagc atgatgcatt ttattcaacg cctgcccaag
      721 aggttttagt tctctcggta atgcgctaac ctcaacaggc tcccggctat cgttataatt
      781 cagcgcttcc gttacaccac tcaacttttt gatctctcgt aatcccgttc tgattaacag
      841 cggacttaat actgagcaaa gtacaatggc gacaatgcaa attataatgc tattaatttt
      901 atactgttca agcatgttat gtctggctga agccaattta gccacagtaa ccgttaatga
      961 tggcgaaact tcatcaatat taattcgtaa agcatcaatc tcagtatcat taataatgct
     1021 tctgtaaatg ccagctgcgc tgattgtctc actagcaggt atgttattta acatgtcatc
     1081 actgacattt gtccggttaa caattttatt gatgccatca ccatgaatga tcaagatatc
     1141 ctgactaaca tccatcatcc ggttaaagta tacaggtaac gtatctggat ttaccccatc
     1201 aattaacaac tgcttgatct gtgctgtccg gttaatgagt gttgtatcat cgcgccattt
     1261 caactcactt gccaggccat tatagagagt ccagacaatt ccagcgccag caacagacag
     1321 cagcaatata aaaagcaagg ttaaacggac ggttatagat aatcttttca ttattttttt
     1381 accgctacga atgaatagcc catcccccgg attgtggcga ttagcttttc aggaaaagga
     1441 tcatcaactt ttgcgcggag cctgcgaatg gcgacatcca ccgtattggt atcactatca
     1501 aagttgattc cccaaatttc actcgcaata accgttctgg gtataatttc gccagctctg
     1561 gaggccagta gccaaagtaa ctgaaactcc ttgcgcgtca gtgtaatact gatattgttc
     1621 ctgctcacac tttgacttac agagtccatt cttaagccac tgatttctaa tgttgaattc
     1681 aaagcgtgat gttgccttaa ttgtgcccga acccttgcca gcaactcaga aaatgaaaaa
     1741 ggttttacca gataatcatt tgctccactg tccagccctc tgactctgtc atcgacagaa
     1801 tcccttgcag taaggcaaat aacaggggtt tgctttgctg ttcttaacgt ttgtaagatc
     1861 tgccagccat ccatacccgg aagcataata tccagaatga tcaatgcata atcatccttc
     1921 agcgcaagat aaagcccatc tctgccatca gaaacggcat caatgacata acccgcttcg
     1981 gaaagcccct gcgttaccca ctcctgggtc ctttgattat cttcaataag taaaatcttc
     2041 atgaaaataa tatgccataa atcatatgtt atagaaacag cctagttcat tacaaaattg
     2101 taatgctgct gtaaggttac cctggccgct ttttcgctat cctcaaaact cattcacatg
     2161 acaaggatat aaacatgtta aagcgttatt tagtactctc cgtagtaacg gcagcatttt
     2221 cattaccttc tttggtttat gccgcacaac aaaacattct tagcgtgcac attttgaacc
     2281 agcaaaccgg aaaacccgct gccgacgtga cagtcactct tgaaaagaaa gcggacaacg
     2341 gctggttaca acttaatacc gccaaaacag ataaggatgg acgaattaag gcactgtggc
     2401 ccgagcaaac tgcaactacg ggcgattacc gtgtcgtatt taaaaccggg gactatttca
     2461 agaaacaaaa tcttgaaagt ttcttccctg agatccccgt tgaatttcat attaataaag
     2521 tgaacgagca ttatcatgtg cctttattac ttagccaata tgggtattca acctatcgtg
     2581 gcagttaatg cataaatatg taaaagcaga tctctgctac tgttagggag gacactccct
     2641 gacagattaa cagtaaacgg ctcttgctgg ctaacgacaa aaaagtgtga tggcttatga
     2701 aaaagaatca atttttaaaa gaatcagatg ttacggccga gtcggtattc tttatgaagc
     2761 gtcgacaggt gttaaaagca ctgggcatca gcgcagctgc actttctttg cctcacgctg
     2821 cgcatgccga tctgcttagc tggtttaaag ggaacgatcg cccgcccgcc cccgccggaa
     2881 aaccgctgga gttcagcaag cctaccgcct ggcaaaataa tctgccactg acgccagtag
     2941 ataaagtctc cggttataac aacttctatg aattcgggct ggataaagcc gatcccgccg
     3001 ctaatgctgg tagcctgaaa accgatccat ggacactgaa aatcagcggc gaagtggcaa
     3061 aaccattgac cctcgatcac gatgatttaa cccgtcgctt cccgctggaa gagcgtattt
     3121 atcgtatgcg ctgcgtggag gcatggtcga tggtggtgcc gtggattggt tttccgctgc
     3181 acaaattgct ggcgcttgcc gaacccacca gcaatgcgaa gtatgtcgct ttcgaaacaa
     3241 tttatgcacc ggaacaaatg ccaggccagc aggaccgctt tatcggcggc gggctgaaat
     3301 atccttatgt cgaaggattg cgtctcgacg aagcaatgca tccgctcaca ctgatgaccg
     3361 tgggtgttta tggcaaggcg ttaccgccac aaaatggcgc gccggtacga ctgattgtgc
     3421 cgtggaaata tggctttaaa gggattaaat cgatagtcag tattaagctg acccgcgagc
     3481 gtccgccaac cacctggaat ctggcagcgc ctgacgaata cggtttttac gccaacgtta
     3541 atccgcatgt tgatcacccg cgctggtcac aggctaccga acgatttatt ggttcaggcg
     3601 gcatcctcga tgttcagcgc cagccaacgc tactgtttaa tggttacgcc gaccaggtgg
     3661 catcgctgta tcgtggcctg gatttgcggg agaatttctg aatgcgtctg acagcaaaac
     3721 aggtgacatg gctgaaagtt agcctgcatc ttgccggatt gttgccgttt ctctggctgg
     3781 tctgggcgat caatcacggt ggactgggtg ccgatccggt gaaagatatt cagcatttta
     3841 ccggtcgcac tgcactgaaa tttttgctgg cgaccttgtt aatcacacct ctggcacgct
     3901 acgcaaaaca gccgttattg atacgcactc gccgcctgtt aggcttatgg tgctttgcct
     3961 gggcgacact gcatttaacc agttacgcat tgctggagct gggcgtgaat aatctggcgt
     4021 tattaggcaa ggagttaatt acccggcctt atttaacgtt aggaattatc agctgggtta
     4081 ttttgcttgc tttagcgttt acttcaaccc aggcgatgca gcgaaaactc ggcaagcact
     4141 ggcaacagtt gcataatttc gtctatcttg tcgcgatcct ggccccaata cattatctgt
     4201 ggtcagtgaa gattatttca ccgcagccgc tcatctacgc tgggctggct gtactgcttt
     4261 tagccttacg gtataagaag ttgcgttccc tttttaagcg gttacgcaag caggttcaca
     4321 acaaattatc tgtataactt catattgccg acaaagtacg cgttatccct gccggatgtg
     4381 gcgcgagcgc cctgtccggc ctgcgcagat cacatcaggt tatgtttcgt ttgtcatcaa
     4441 cctcaaatta tcaacataat tcattttatt tcaataagat agataagtac acctgagaaa
     4501 actatgctct catctcctaa gagttgttgc attttgccat atgttacaat ataacattac
     4561 acatcatata caatgactct ggaggaaact gttttggcaa ttcgtcttca caaactggct
     4621 gttgctttag gtgtctttat tgttagcgct cctgcctttt cgcatggtca tcactcacac
     4681 ggcaaaccct taacagaggt cgaacaaaaa gctgctaatg gtgtttttga tgatgccaat
     4741 gtacaaaacc gaacgctcag tgactgggat ggagtctggc aatccgttta tcctttactg
     4801 caaagtggca aacttgaccc cgtctttcag aagaaagcgg atgcagataa aattaaaaca
     4861 tttgctgaaa ttaaagatta ttatcacaaa ggttatgcaa cagatatcga gatgattggc
     4921 attgaggacg gcattgttga attccataga aataatgaaa caacatcctg taaatatgat
     4981 tacgatggat acaaaatact cacctataaa tcaggcaaga aaggcgttcg ttacttattc
     5041 gaatgtaaag atcctgaaag caaagcccct aaatatatac aatttagcga tcatataatt
     5101 gcaccacgaa aatcatctca ttttcacatt tttatgggta atgattcaca gcaattttta
     5161 ttaaatgaaa tggaaaactg gccaacgtat tatccatatc agttgagtag cgaagaagtg
     5221 gtcgaggaaa tgatgtctca ttgagtattc tcatgataac gcctcgatgc cgctttagta
     5281 agtcatcata actgccactg gtccatccac aaacgccact gaacgcaagc tagctacaga
     5341 cacgctcatc actatgacgt gtctgtatat taataagcta acccgcattg agttaaccaa
     5401 taacggattc catacacaat acggccaaca gagaaagata cctgtgctca cgccattgct
     5461 tatattggct ggttacaatg tgcactatca attttttaaa taacaaattc ttaaaaccac
     5521 atctgtataa ggaattttta aggttcgtgg gatagcttga ctgtaaaaat tacaggagct
     5581 acaaaaatga accgattctc aaaaactcaa atttatttac attggataac gctgcttttc
     5641 gttgcaataa cctatgccgc gatggaactc cgtggctggt ttcctaaagg tagtagtact
     5701 tatctgctga tgcgagaaac acattacaat gcgggtatat tcgtttgggt gttaatgttt
     5761 tcacgcctga ttataaaaca ccgttatagt gatccttcta ttgtaccacc gccacctgcc
     5821 tggcaaatga aagcggcttc gctaatgcac atcatgcttt atataacctt ccttgcactt
     5881 cctctgctgg ggattgcttt gatggcttac agtggaaaat cgtggagttt ccttggtttc
     5941 aatgtgtctc cctttgttac cccaaacagc gaaattaaag cactgataaa aaatattcac
     6001 gaaacctagg caaatatagg ttacttttta atcgcagctc acgctggcgc agcactcttt
     6061 catcactaca ttcagaaaga taatactctg ttacgaatga tgcatcgccg caaataagtc
     6121 gatcccctga caaggaaatg tgaaccatgt acatcaacgc gtcataaatg tttacgcaag
     6181 tgcacaatgc atcctttcta ctatcaacaa ggaggagcag aaactgtgta aatcgtacaa
     6241 tggtaagact tatcaaaaat acaggttaat tttggtattt aaaccttttt tacttgttag
     6301 tgtgatacaa aggctttcaa aaaagctggg aaattcaaca actcttggaa attatggact
     6361 ttgtcccgcg gctttaatct gacggccgcg ttcctttttt gactcaatta ttgacccctt
     6421 ctctacgcaa cttcagttcc caccaccaac tttgcggcag tttcatagga tgaatgtcta
     6481 aaagaataat ggtgagcgat aagaaaacga ctgaataact gcacattttc gctcgaaagc
     6541 ttcctgtcag atccatagcg aatcaagtgc tgaatgtcac agtatcgaac agaaaacagt
     6601 gacgatctaa cccttcaaga atattctaca attgttctgt ttaggaaaag caaggcggga
     6661 agtcaggaga taagtcattg ataaagtggc ggagagaggg ggatttgaac ccccggtaga
     6721 gttgccccta ctccggtttt cgagaccggt ccgttcagcc gctccggcat ctctccgttc
     6781 agatggttgc catgatgcca ggaaatttgg cattttaaca gtccctgttc gtgcaatttt
     6841 gttcaagtga cgagtttgcg agcaaaacga tgattaagtg gccctggaaa gtacaagaat
     6901 cagcacatca aactgccctt ccctggcagg aagcactatc gatccccctt ttaacgggtc
     6961 tgacggaaca ggaacaaagc aaattagtca ctcttgccga acgtttttta cagcaaaagc
     7021 ggcttgttcc tttacagggc tttgaactgg attcattaag aagctgccgg atagcacttc
     7081 tattttgcct acccgttctg gagttaggac tggaatggct ggatggtttt catgaagtct
     7141 taatttatcc tgcgccattt gtggtcgatg atgaatggga agacgatatc ggtctggtgc
     7201 ataaccaacg tattgttcag tcaggtcaga gctggcagca agggcctatc gttttgaact
     7261 ggttggatat acaagattct tttgatgcat ctggttttaa cctgattatt catgaagtcg
     7321 ctcataagct ggacacccgt aacggcgatc gcgccagcgg agttcccttt attccgttgc
     7381 gtgaggttgc tggctgggaa cacgatcttc atgctgcaat gaacaacatt caggaagaaa
     7441 tcgaattggt tggcgagaat gcggcgagca ttgatgctta tgcagccagc gatcctgctg
     7501 aatgttttgc cgtactttct gaatatttct ttagcgcccc agaacttttt gctcctcgtt
     7561 tcccttcatt gtggcaacgt ttctgccaat tttatcaaca agatcctttg cagagactgc
     7621 atcacgctaa tgatacagac tcgttttcgg cgacgaatgt tcattaatta acaactttgc
     7681 agattaatta accaattgaa atgacttatg aaatttagtg ttgacagaca aggtaccgct
     7741 aagtaatatg tgccccgttc acacgattcc tctgtagttc agtcggtaga acggcggact
     7801 gttaatccgt atgtcactgg ttcgagtcca gtcagaggag ccaaattcta aaaattcgct
     7861 tttttagcgc aatgtcactg accttagttg aacattgttt tttaacggat agcgggtttt
     7921 taacacctta agcgccctcg acctttatgg ttgagggcgt tttgctatga acgccatcac
     7981 cattttcccc tcgattataa aacttgagtt attcagtagt ctcccctctt gcaactcaca
     8041 cccaaaactg cctaacgaaa agttattaat tttcaatcgt attgctatca gtatttacat
     8101 tttttcgctg tgctagaaag ggcgcattta tgttagctcg ttcagggaag gtaagcatgg
     8161 ctacgaagaa gagaagtgga gaagaaataa atgaccgaca aatattatgc gggatgggaa
     8221 ttaaactacg ccgcttaact gcgggtatct gtctgataac tcaacttgcg ttccctatgg
     8281 ctgcggcagc acaaggtgtg gtaaacgccg caacccaaca accagttcct gcacaaattg
     8341 ccattgcaaa tgccaatacg gtgccctaca cccttggagc gctggaatcg gcccaaagcg
     8401 ttgccgaacg tttcggtatt tcggtggctg agttacgcaa actcaaccag tttcgtacgt
     8461 ttgctcgagg ttttgataat gtccgccagg gtgatgaact ggatgtcccg gcacaagtta
     8521 gtgaaaaaaa attaaccccg ccgccgggta ataacagtga caacctcgag caacagatag
     8581 ccagtacttc acagcaaatc gggtctctgc tcgccgaaga tatgaacagc gagcaagcgg
     8641 caaatatggc gcgtggatgg gcctcttctc aggcttcagg cgcaatgaca gactggttaa
     8701 gccgcttcgg taccgcaaga atcacgctgg gcgtggatga agattttagc ctgaagaact
     8761 cccagttcga ttttctccat ccgtggtatg aaacgcctga taatctcttt ttcagtcagc
     8821 atactctcca tcgtactgac gagcgtacgc agattaacaa cggcttaggt tggcgtcatt
     8881 tcactcccac atggatgtcg ggcatcaact tctttttcga ccacgatctt agccgttacc
     8941 actcccgcgt cggcattggc gcggagtact ggcgcgacta tctaaaatta agcagtaacg
     9001 gctatttgcg actgaccaac tggcgcagcg cacctgaact ggacaacgat tataaagcac
     9061 gcccggccaa tggctgggat gtacgcgcag aaagctggct acccgcctgg ccgcaccttg
     9121 gcggtaaact ggtctatgaa cagtattatg gcgattaagt ggccctgttc gataaagacg
     9181 atcggcaaag taatcctcat gccataaccg ctggacttaa ctataccccc ttcccgctga
     9241 tgaccttcag cgcggagcaa cgccagggta aacagggcga aaatgacacc cgttttgccg
     9301 tcgattttac ctggcaacct ggcagcgcaa tgcagaaaca gcttgacccg aatgaagtcg
     9361 ctgcacggcg tagccttgca ggcagccgtt atgatctggt ggatcgcaac aacaatatcg
     9421 ttctggaata tcgcaaaaaa gaactggttc gcctaaccct gacagacccc gtgacaggga
     9481 agtcaggaga agtgaaatca ctggtttcgt cgctacaaac caaatatgcc ctgaaaggct
     9541 ataacgtcga agccaccgca ctggaagctg ccggtggcaa agtggtcaca acgggtaaag
     9601 atattctagt taccctgccg gcttaccggt tcaccagtac gccagaaacc gataacacct
     9661 ggccgattga agtcaccgcc gaagatgtca aaggcaattt gtcgaatcgt gaacagagca
     9721 tggtggtcgt tcaggcacct acgctaagcc agaaagattc ctcggtatcg ttaagtaccc
     9781 aaacattgaa cgcggattcc cattcaaccg ccacactgac ttttattgcg catgatgcag
     9841 caggtaatcc tgttgtcggg ctggtgctct cgacgcgtca cgaaggtgtt caggacatca
     9901 ccctttctga ctggaaagat aatggtgacg gaagctatac ccagatcctg accacaggag
     9961 cgatgtctgg cacgctgacg ctgatgccac agctgaatgg tgtggatgcg gctaaagccc
    10021 ccgccgtggt gaatatcatt tctgtttcgt catcccgaac tcactcgtca attaagattg
    10081 ataaggaccg ttatctctcc ggcaatccta tcgaggtgac ggtagaactg agagatgaaa
    10141 atgacaaacc tgttaaggaa caaaaacagc aactgaataa cgcagtcagc atcgacaacg
    10201 tgaaaccagg agtcactaca gactggaaag aaaccgcaga tggcgtctat aaggcgacct
    10261 ataccgccta taccaaaggc agtggactta ctgcgaagct attggaatga agatttgcat
    10321 accgctggat ttatcatcga cgccaacccg cagtcagcga aaattgcgac attatctgcc
    10381 agcaataatg gtgtactcgc caatgagaat gcagcaaaca ccgtctcggt caatgtcgct
    10441 gatgaaggaa gcaacccaat caatgatcat accgtcacgt ttgcggtatt aagcggatcg
    10501 gcaacttcct tcaacaatca aaacaccgca aaaacggatg ttaatggtct ggcgactttt
    10561 gatctgaaaa gtagtaagca ggaagacaac acggttgaag tcacccttga aaatggcgtg
    10621 aaacaaacgt taatcgtcag ttttgtcggc gactcgagta ccgcgcaggt tgatctgcag
    10681 aagtcgaaaa atgaagtggt tgctgacggc aatgacagcg ccacaatgac cgcgaccgtc
    10741 cgggatgcaa aaggcaacct gctcaatgac gtcatggtca ctttcaatgt taattcagca
    10801 gaggcgaaac tgagccaaac cgaagtgaat agccacgacg ggatcgccac agctacgctg
    10861 accagtttga aaaatggtga ttatagggtt acggcctctg tgagctctgg ttcccaggct
    10921 aatcaacagg tgaattttat cggtgatcaa agtactgctg ccctgaccct cagtgtgcct
    10981 tcaggtgata tcaccgtcac caacacagct ccgcaacata tgactgcaac cttgcaggat
    11041 aaaaatggca acccactaaa agataaagaa atcaccttct ctgtgccaaa cgacgtcgca
    11101 agtaagttct cgattagcaa cggaggaaaa ggcatgacgg atagtaacgg ggttgcaatc
    11161 gcctccctga ccggcacgtt agcgggcacg catatgatca tggctcgtct ggctaacagc
    11221 aatgtcagcg atgcacagcc aatgacgttt gtggcggata aagacagagc ggttgtcgtt
    11281 ttgcaaacat cgaaagcgga aatcattggg aatggcgtgg atgagacaac tctgacagca
    11341 acagtgaaag atccgtcgaa tcatccggtg gcggggataa cggtaaactt caccatgcca
    11401 caggacgttg cggcaaactt tacccttgaa aataacggta ttgccatcac tcaggccaat
    11461 ggggaagcgc atgtcacgct gaaaggtaaa aaagcgggca cgcatacggt taccgcaacg
    11521 ctgggtaata acaataccag tgattcgcag ccggtaacat ttgtggcgga caaagcctcg
    11581 gctcaggttg tcctgcagat atcaaaagat gagatcacag gtaatggcgt cgatagcgca
    11641 acgctaactg caacggttaa agatcagttc gacaatgagg tgaataatct tccggtaaca
    11701 ttcagctcag cctcttcagg actcaccctg accccgggag taagtaatac caatgagtct
    11761 ggcatcgcgc aggccactct cgcaggcgtt gcctttggtg agcagacggt cactgcatca
    11821 ctggctaata atggtgccag cgacaacaaa actgtgcatt ttattggcga cacagcggcg
    11881 gcaaaaatta tcgagttgac gcctgtccca gacagcataa tcgccggtac cccgcagaac
    11941 agctccggca gcgccatcac cgccacagtc gttgataata atggctttcc ggtgaaaggt
    12001 gtgactgtga acttcaccag cagaacaaac tctgccgaaa tgacgaatgg cggccaagcc
    12061 gtaacgaacg aacagggtaa ggctaccgtc acttatacca atacccgctc ctcgatagaa
    12121 tcaggagcga gaccggatac cgttgaggcc agtctggaaa atggtagctc cacgcttagc
    12181 acatcaatta atgtcaacgc tgatgcgtct acggcacatc tcacctggct acaggcactt
    12241 tttgatacag tctccgcagg cgagacaacc agtctgtata ttgaggtgaa ggataattac
    12301 ggcaacggtg tcccccagca tcaggtgacc ctcagcgttt caccaagtga aggcgtgacc
    12361 cccagtaata acgctatata tactaccaac cacgacggca atttttacgc aagctttacc
    12421 gctacaaaag ccggggttta tcaagtgacg gcaaccctcg aaaatggcga ttcgatgcaa
    12481 caaacagtga cctatgtgcc gaacgtcacg aatgccgaaa tcacgctggc aacctcgaag
    12541 gatccggtga ttgccgacaa taacgatctc acgacactaa cagcaacagt cgctgataca
    12601 gagggcaatg cgatagccaa cactgaagta acatttactc tgccggaaga tgttaaggcg
    12661 aacttcacgc tgagcgatgg cggtaaagcg attactgatg ctgaaggcaa agcgaaagtc
    12721 acgctgaaag gtacaaaagc aggcgctcat actgttacag catcgataac tggcggtaag
    12781 agtgagcagt tggtggtgaa ctttactgcg gatacgctca ctgcgcaggt taatcttaac
    12841 gttaccgagg acaattttat cgctaataac gtcgggatga ccaggctgca ggcaacagtg
    12901 actgatggaa acggcaaccc gttagccaat gaggcggtga cattcacgct accggcagat
    12961 gtgagcgcaa gctttactct cggacaaggc ggttccgcca ttactgacat caacggcaag
    13021 gctgaagtta cactgagcgg tacaaaatcc ggcacctacc ccgtgacagt tagcgtgaac
    13081 aattatggtg tcagtgatac gaaacaggtg actttgattg ccgatgctgg taccgcaaca
    13141 ctagcctcct taacctctgt atactcattc gtcgtcagca cgaccgaagg cgcgaccatg
    13201 actgcaagcg tcactgacgc taacggcaac ccggtagaag gcataaaagt taatttccgc
    13261 ggaacctccg tcacgctaag cagcaccagc gttgaaacgg atgatcgggg tttcactgaa
    13321 attcttgtga caagcaccga agtcggactg aaaacagttt cagcctctct ggcagataaa
    13381 cctactgaag tcatatcgcg attactgaat gcaaaagcag atattaattc tgcaacgatt
    13441 accagtctgg agatacctga aggtcaggtc atggtcgcac aagacgtagc agttaaagct
    13501 cacgtcaacg accagtttgg caacccgatt cttaatgaat ctgtaacatt cagtgcagag
    13561 ccaccagagc acatgaccat cagccaaaat attgtctcta ctgatacgca tggtatagcc
    13621 gaggtctcca tgacgcccga aagaaacggt tcgtatatgg tgaaagcatc cctggcgaat
    13681 ggagcctcac ttgagaaaca actggaggct attgatgaaa aactgacact ctcggcgtcc
    13741 agcccgctta tcggtgtcaa ttccccaaca ggtgcaactc tgacggcaac gctaacttct
    13801 gcaaatggca ctccagtgga gggtcaggtc atcaacttta gcgtaacgcc agaaggtgcg
    13861 acgttaagtg gcggaaaagt gagaactaac tcttctggtc aggctccagt cgttctgacc
    13921 agcaataaag tcggtacata tacggtgact gcatcgttcc ataacggcgt aacaatacag
    13981 acacagacaa ccgtgaaagt cactggcaac tcaagcaccg cccatgttac tagctttatc
    14041 gctgatccat cgactatcgc cgccaccaac agtgatttaa gtaccttaaa ggcaacggtt
    14101 gaggatg
//
LOCUS       DAJTZL010000109        14081 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.109, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000109 DAJTZL010000000
VERSION     DAJTZL010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14081
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.109"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            75..2216
                     /gene="ldcC"
                     /locus_tag="OO544_002761"
     CDS             75..2216
                     /gene="ldcC"
                     /locus_tag="OO544_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HCR6768314.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVEMAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLPGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            2273..2662
                     /locus_tag="OO544_002762"
     CDS             2273..2662
                     /locus_tag="OO544_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR6768315.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            2727..4025
                     /gene="tilS"
                     /locus_tag="OO544_002763"
     CDS             2727..4025
                     /gene="tilS"
                     /locus_tag="OO544_002763"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HCR6768316.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVRHCENICQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMGEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRFGAFEIRRYQSQLWWIKSVTGQSETIVPWQTWLQPLELPA
                     GLGSVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(4074..4334)
                     /gene="rof"
                     /locus_tag="OO544_002764"
     CDS             complement(4074..4334)
                     /gene="rof"
                     /locus_tag="OO544_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HCR6768317.1"
                     /translation="MSMNDAYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(4321..4521)
                     /locus_tag="OO544_002765"
     CDS             complement(4321..4521)
                     /locus_tag="OO544_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HCR6768318.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            4687..5232
                     /locus_tag="OO544_002766"
     CDS             4687..5232
                     /locus_tag="OO544_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HCR6768319.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADYEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAKV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            5229..5590
                     /gene="arfB"
                     /locus_tag="OO544_002767"
                     /pseudo
     CDS             5229..5590
                     /gene="arfB"
                     /locus_tag="OO544_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
     gene            5664..6374
                     /gene="nlpE"
                     /locus_tag="OO544_002768"
                     /gene_synonym="cutF"
     CDS             5664..6374
                     /gene="nlpE"
                     /locus_tag="OO544_002768"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HCR6768320.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(6529..7353)
                     /locus_tag="OO544_002769"
     CDS             complement(6529..7353)
                     /locus_tag="OO544_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HCR6768321.1"
                     /translation="MDKPKAYCRLLLPSFLLLSACTVDISQPDPSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITVPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIYLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSSYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSGLAKAQLSSVGEGDKKSWFCSEFVTDAFAKA
                     GHPLTLAQSGWISPADLMHMRTGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTQ"
     gene            complement(7406..9124)
                     /gene="proS"
                     /locus_tag="OO544_002770"
     CDS             complement(7406..9124)
                     /gene="proS"
                     /locus_tag="OO544_002770"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HCR6768322.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(9235..9942)
                     /gene="tsaA"
                     /locus_tag="OO544_002771"
     CDS             complement(9235..9942)
                     /gene="tsaA"
                     /locus_tag="OO544_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HCR6768323.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(9939..10343)
                     /gene="rcsF"
                     /locus_tag="OO544_002772"
     CDS             complement(9939..10343)
                     /gene="rcsF"
                     /locus_tag="OO544_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HCR6768324.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTLGCYRQAVCIGSALNITAK"
     gene            complement(10461..11276)
                     /gene="metQ"
                     /locus_tag="OO544_002773"
     CDS             complement(10461..11276)
                     /gene="metQ"
                     /locus_tag="OO544_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HCR6768325.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(11316..11969)
                     /gene="metI"
                     /locus_tag="OO544_002774"
     CDS             complement(11316..11969)
                     /gene="metI"
                     /locus_tag="OO544_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HCR6768326.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(11962..12993)
                     /gene="metN"
                     /locus_tag="OO544_002775"
     CDS             complement(11962..12993)
                     /gene="metN"
                     /locus_tag="OO544_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HCR6768327.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            13181..13756
                     /gene="gmhB"
                     /locus_tag="OO544_002776"
     CDS             13181..13756
                     /gene="gmhB"
                     /locus_tag="OO544_002776"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HCR6768328.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
BASE COUNT         3430 a         3560 c         3736 g         3355 t
ORIGIN      
        1 cacttcggtg gccctttttt atcgccacgg tttgagcagg ctatgattaa ggaaggattt
       61 tccaggagga acacatgaac atcattgcca ttatgggacc gcatggcgtc ttttataaag
      121 atgagcccat caaagaactg gagtcggcgc tggtggcgca aggctttcag attatctggc
      181 cacaaaacag cgttgatttg ctgaaattta tcgaacataa ccctcgaatt tgtggcgtga
      241 tttttgactg ggatgagtac agtctcgatt tatgcagcga tatcaatcaa cttaatgaat
      301 atctcccgct ttatgccttc atcaacaccc actcgacgat ggatgtcagc gtgcaggata
      361 tgcggatggc gctctggttt tttgaatatg cgctggggca ggcggaagat atcgccattc
      421 gtatgcgtca gtacaccaac gaatatcttg ataacattac gccgccgttc acgaaagcct
      481 tgtttaccta cgtcaaagag cggaagtaca ccttttgtac gccggggcat atgggcggca
      541 ccgcatatca aaaaagcccg gttggctgcc tgttttatga ttttttcggc gggaataccc
      601 tcaaggctga tgtctctatt tcggtcaccg agcttggttc gttgctcgac cacaccgggc
      661 cacacctgga agcggaagag tacatcgcgc ggacttttgg cgcggaacag agttatatcg
      721 ttaccaacgg aacatcgacg tcgaacaaaa ttgtgggtat gtacgccgcg ccatccggca
      781 gtacgctgtt gatcgaccgc aattgtcata aatcgctggc gcatctgttg atgatgaacg
      841 atgtagtgcc agtctggctg aaaccgacgc gtaatgcgtt ggggattctt ggtgggatcc
      901 cgcgccgtga atttactcgc gacagcatcg aagagaaagt cgctgccacc acgcaggcac
      961 aatggccggt tcatgcggtg attaccaact ccacctatga tggcttgctc tacaacaccg
     1021 actggatcaa acagacgctg gatgtcccgt cgatccactt cgattccgcc tgggtgccgt
     1081 acacccattt tcatccgatc taccagggga aaagtggtat gagcggcgag cgtgttgcgg
     1141 gaaaagtgat cttcgaaacg caatcgaccc acaaaatgct ggcggcgtta tcgcaggctt
     1201 cgctgatcca cattaaaggc gagtatgacg aagaggcgtt taacgaagcc tttatgatgc
     1261 ataccaccac ctcgcccagt tatcccattg ttgcttcggt tgagatggcg gcggcgatgc
     1321 tgcgtggtaa tccgggcaaa cggctgatta accgttcggt ggagcgggcg ctgcattttc
     1381 gcaaagaggt ccagcggttg cgggaagagt ctgacggttg gtttttcgat atctggcaac
     1441 cgccgcaggt ggatgaagcc gaatgctggc ccgttgcgcc aggcgaacag tggcacggct
     1501 ttaacgatgc ggatgccgat catatgtttc tcgatccggt taaagtcact attttgacac
     1561 cggggatgga cgaacagggc aatatgagcg aggaggggat cccggcggcg ctggtggcaa
     1621 aattcctcga cgaacgtggg atcgtagtag agaaaaccgg cccttataac ctgctgtttc
     1681 tctttagtat tggtatcgat aaaaccaaag caatgggatt attgcgtggg ttgacggaat
     1741 ttaaacgctc ttacgatctc aacctgcgga tcaaaaatat gctacccgat ctctatgctg
     1801 aagatcccga tttctaccgc aatatgcgta ttcaggatct ggcgcaaggg atccataagc
     1861 tgattcgtaa acacgatctt cccggtttga tgttgcgggc attcgatact ttgccggaaa
     1921 tgatcatgac gccacatcag gcatggcaga gacagattaa aggtgaagta gaaaccattg
     1981 cgctggaaca actggtcggt agagtatcgg caaatatgat cctgccttat ccaccgggcg
     2041 taccgctgtt gatgccagga gaaatgctga ccaaagagag ccgcacggta ctcgattttc
     2101 tactgatgct ttgttccgta gggcaacatt accccggttt tgaaacggat attcacggcg
     2161 cgaaacagga cgaagacggc gtttaccgcg tacgagtcct aaaaatggcg ggataacttg
     2221 ccagagcggc ttccgggcga gtaacgtgct gttaacaaat aaaggagacg ttatgctggg
     2281 tttaaaacag gttcaccata ttgcgattat tgcgacggat tatgcggtga gcaaagcttt
     2341 ctactgcgat attcttggtt tcacgctgca aagcgaagtc tatcgcgaag cgcgcgactc
     2401 gtggaaaggg gatttggcgc ttaatgggca atatgtgatt gagcttttct cttttccgtt
     2461 cccgccggag cgccccagcc gaccggaagc ttgcggcctg cgtcatctgg cttttagcgt
     2521 tgatgatatc gatgcggcag tggcgcacct tgaaagccat aacgtgaagt gtgaagccat
     2581 ccgtgtcgat ccatacacgc aaaaacgctt cacgttcttt aacgatccgg acgggctgcc
     2641 gttggaactg tatgagcagt aaggcttgtc atcgccgcat ttgcccggta acgtgccggg
     2701 cattgctact gtaaaatcgc accatcatga cactcacgct caatagacaa cttctcacct
     2761 cacgccagat tctggtggcc tttagcggcg ggcttgactc caccgttctg ctgcatcagt
     2821 tggtgcagtg gcggacggaa aatccgggcg tcactctacg cgctatccat gtgcatcatg
     2881 gtttaagtgc taatgccgat gcctgggtta ggcattgcga gaacatctgc caacagtggc
     2941 aggtgccgct ggtggtcgag cgcgtgcaac ttgcgcaaga aggactgggc attgaggccc
     3001 aggcgcggca ggcgcgttat caggcatttg ctcgtaccct gttacccggt gaagtgctgg
     3061 tcaccgcgca acatctcgac gatcaatgtg aaacctttct gctggcgcta aaacgcggta
     3121 gcggccctgc cgggctttcg gctatggggg aagtttcgga atttgccgga acacggctta
     3181 tccgcccgtt gcttgcccgc acgcgggggg aactggagca gtgggcattg gcgcatggtt
     3241 tacgctggat tgaagacgaa agtaatcagg acgactcata cgatcgtaac tttctgcgcc
     3301 tgcgtgtagt gccgttattg cagcagcgtt ggccgcattt tgccgaagca acggcccgca
     3361 gcgccgcact ttgtgctgaa caagagagtc tgttggatga actgctggcg gatgatttag
     3421 cacactgcca aacgtcgcag gggacgctgc agattgcgcc aatgctggcg atgagtgatg
     3481 cccgtcgcgc ggcgattatc cgccgctggc tggcagggca gaatgcaccg atgccttccc
     3541 gcgacgcgct ggtgcggatc tggcaggaag tggcgctggc gcgggaagat gcctccccct
     3601 gtttacgttt tggcgcgttt gaaatacggc gctatcagtc gcaactgtgg tggattaaat
     3661 ccgtcactgg gcaaagcgaa accattgtgc cgtggcagac gtggctacaa ccgctggaat
     3721 taccggcggg gctgggaagt gtacagctta ctgcgggagg cgatattcgc cctccgcgtg
     3781 cagacgaagc ggtcagcgtg cgtttcaaag cgccaggatt gctgcatatt gtcgggcgta
     3841 acggcggacg taagctaaag aaaatctggc aagagctggg cgtgccgccg tggctacgtg
     3901 acaccacgcc actgctgttt tatggcgaaa cgctgattgc ggcggcaggg gtatttgtga
     3961 cgcaagaagg tgtggctgaa ggtgagaatg gcgtaagttt tgtctggcag aaaacgctta
     4021 gttaagtgaa agccggataa gacgcatcaa acgtcgcatc cggcgaaagt caatcaggat
     4081 tcgcttacca ccaccgtacc gatttccggg tggctaaagc tggtaatttt atccagacgc
     4141 agctcgcggg tttcgccagc ggcctcgacg accagatact ccacattttt gcgggagact
     4201 aaatcactgg cttttgcctg caatttttcg ccatctttca gctcaagtgt cagcattaaa
     4261 tgatgctggc aggcgagctc aagattatcg taatcatcac aattgattgg ttgatacgca
     4321 tcattcattg acataatcgc tcaccagtaa gtttgccgca gcgtatgctg ctttttccct
     4381 gacagcctca gaaagggcgt cgtcggcagc catttcattc agcactttca aaacgcagcc
     4441 cagcgcgtcc ggaacgtatc ctaagtctcc gctggcgatt tccgcgtacc gcttgcgtat
     4501 taactcacaa tatttttcca catgccctcc tgtcagcact ctgacttaac cgtggatgca
     4561 agtctaagcc tacgaagata aactctgttt cgcaaggtga ctataccaca ctcatttctg
     4621 caatatcagc gccgcaactg cacgtattcc gttacaatgg cctcctgatt cgaaaggagt
     4681 tttcttatgg cgcttaaagc gacaatttat aaagcgacgg ttaatgtggc cgatctcgac
     4741 cgcaaccagt ttctcgatgc ctctctgacg ctggcgcgcc acccttcaga aacccaggag
     4801 cgtatgatgc tgcgcctgct ggcgtggctg aaatatgctg atgaacgtct gcaatttacc
     4861 cgtggtttgt gtgccgatta tgagccggaa gcgtggctgc gtaacgatca cctgggcatt
     4921 gatttgtgga ttgagctggg gctgccggat gagcggcgga ttaagaaagc ctgcacccag
     4981 gccgcaaaag tggcgctgtt tacctataat agccgggcgg cgcaaatctg gtggcagcaa
     5041 aatcagagca aatgtgtgca gtttgccaat ctttccgtct ggtatctgga cgatgaacaa
     5101 ctggcgaaag taagcgcctt tgccgatcgt accatgacgc tgcaggcaac gattcaggat
     5161 ggcgtgatct ggttatcgga tgataagaat aatctggaag tgaacttaac cgcctggcaa
     5221 caaccttcat gattgtgatt tcccgacatg ttgctattcc cgatggtgag cttgagatca
     5281 ccgccattcg tgcgcagggc gcgggcgggc agcatgttaa taagacctca acggctattc
     5341 atctgcgttt tgacattcgg gcgtccagcc tgccagagta ttacaaagag cgtctgctcg
     5401 ccgccagcca tcatttgatc agcatgatgg cgtaattgtc attaaggcac aggaataccg
     5461 cagtcaggaa ctgaaccgcg aagcggcgct ggcccggctg gtggcagtga ttaaagattt
     5521 aacaacagaa caaaaagccc gacgacccac gcggcccacc cgtgcatcga aagagcgcag
     5581 gctggcatag aaagcacaaa aatcaagcgt gaaggcgatg cgcggcaaag tacgcagcgg
     5641 tcgggaataa aaagaaggaa tggatggtga aaaaagcgat agtgacagcg atggctgtaa
     5701 tcagcctctt tactctgatg ggatgtaata atcgggccga agtcgatacg ctttctccgg
     5761 cgcaggctgc cgaactaaaa ccgatgccgc aaagttggcg cggcgtgctg ccgtgtgccg
     5821 attgcgaagg gatcgaaacc tctctgttcc tcgaaaaaga cggaacatgg gtgatgaatg
     5881 agcgttatct cggtgctcgt gaagaacctt cctccttcgc ttcctacggt acatgggcgc
     5941 gaaccgctga caagctggta ttaaccgaca gcaaaggtga aaagtcatat tatcgggcga
     6001 aaggcgatgc gctggagatg ctcgatcgtg aaggcaatcc aattgaatcg cagttcaact
     6061 atacgctgga accggcacaa tccagtttac ctatgacgcc gatgaccctg cgaggcatgt
     6121 atttttatat ggctgatgcg gcgaccttca ctgattgcgc gaccggaaaa cgtttcatgg
     6181 tagcgaataa cgcagagctg gagcgtagct acctggctgc gcgcggtcac agtgaaaaac
     6241 cggtgttact gtcagtagaa ggtcacttta cgcttgaggc taatccggat actggtgcgc
     6301 caaccaaagt attagcgccc gatacggcgg gtaaatttta cccgaataag gattgcagta
     6361 gtttggggca gtaaacctgc ttaaggcaga aacaaacgca aaattgcctg atgcgctgcg
     6421 cttatcaggc ctacagcata taatgcaaca ttttgaatta gcacgatttt gtaggccgga
     6481 taaggcgttc acgacgcatc cggcattttg tgcacgaggc tggctttatc actgagtcag
     6541 cccaacaaag cgacccgcct tgatataaat ccctggcttc aggtgcccaa catactgtaa
     6601 ctgcgtttct ggcttaaacg cagagacatc gccagtacgc atatgcatca gatcggcagg
     6661 gctaatccag cctgattgcg ccagcgtaag cggatgcccg gctttggcaa atgcatcagt
     6721 gacaaattcc gaacaaaacc acgacttttt gtctccttcg cccacactgc ttaactgcgc
     6781 tttcgccagg ccgctgacgc actgttggcg aaaatcctcg gagaacggat tcagtgagca
     6841 catctggcga gtcaccataa agggaataaa ttcgacaatg ccgcgatagt tataactgct
     6901 atctttgatt ttattggcaa acgcggtgat atctgtggct tgttgcgggg taagatccgg
     6961 gactcgtaag acgaaaagct tatcactatg cttcatcgct tttttaaggg aaacgatctg
     7021 gacgccagcg cctgtcgctt ctgcaacgtt attatcaccc agatagattg caacgtgact
     7081 cacagaggaa gtgctgaaga cgcggattcc aaatgaggtt accccaaggc ttgaggagaa
     7141 cagcaaatcg ccgggtttga gatcaggtac cgttatttct tttattgatt gttcggtgaa
     7201 agagctttga tgctggaatt tgacagccca tgtttttgcc tcggcatcta ctgctgtggc
     7261 tgatgggtct ggctggctga tatcaacggt acaagccgaa agtaaaagaa agctggggag
     7321 aagcaggcgg cagtacgcct ttggtttatc cattttatac aatccatgta aaaaagggcc
     7381 ctgaaattca ggaccctttc tggcatcagc ctttaatctg tttcaccaga tattcgacga
     7441 tgtcaccagt cttaattaac tgtttctcgc cgttgcgacg atatttatat tcgatatcgt
     7501 cgttgtcgag gttacggtcg cccagcacaa tagtgtgcgg aataccgatc agttccatat
     7561 cagcaaacat cacgcccgga cgctctttgc ggtcatccag cagcacttcg ataccttgtg
     7621 cacgcagttc gctgtacagt ttctcagcaa gctcttgtac gcggaaggat ttgtgcatgt
     7681 tcatcggcag aatcgccacc tggaacggcg cgatagcgtc aggccatacg atgccgcgtt
     7741 cgtcgtagtt ctgctcaatc gccgcagcta ccacacgcgt taccccgata ccgtagcaac
     7801 ccatcgtcag gatttggtta cggccatctt caccctgtac ggaggctttc agtgcttcgg
     7861 agtacttggt acccagctgg aagatgtgac caacttcgat accacgtttg atcagcagcg
     7921 taccctggcc atccgggctt ggatcgccag ccaccacgtt acggatatct gcaacttccg
     7981 gggtagcgac atcgcgatcc cagttgatac cgaagtagtg tttaccatcg atgttagcac
     8041 cagcagcgaa atcactcatc gccgcaacgg tacggtcaat caccaccgga atcggcatgt
     8101 ttaccggacc cagtgaaccc ggaccggctt taaccacagc acgaatttct tcttcggtcg
     8161 cgaaagtcag cgggcttgca acctgcggca gtttttctgc tttaacttcg ttcagctcgt
     8221 gatcaccgcg caccagcagc gcaaccagcg ggaagctgct gccttcaacc gctttaacca
     8281 gcagagtctt aaccgttttc tcaatcggca gattgaactg ttcaaccagt tccgcgatgg
     8341 ttttcgcgtt cggcgtatca accagcgtca tttcctgggt agcagcagcg cgcggttctt
     8401 tcggcgcgat agcttctgcc agttcaatgt tcgctgcata gtcagaggtg tcggagaaga
     8461 ccacatcgtc ttcaccgctc tgcgccagca cctggaattc gtgagaggcg ctgccgccga
     8521 tagaaccggt gtcggcttgc acggcgcgga aatccagccc catgcggctg aagattttgc
     8581 tgtaggccgc atacattgca tcgtaggttt cctgcaggga ttcctgagaa gtatggaaag
     8641 agtaagcatc tttcatcagg aattcgcggg aacgcatgac gccgaaacgc ggacgcactt
     8701 cgtcgcggaa cttggtctgg atctgataga agttcagcgg cagctgtttg taagagctaa
     8761 gctcgttacg aatcaggtca gtgataactt cttcatgagt tgggccgagt acgaacggac
     8821 gctcgccacg gtcaacaaaa cgcagcagtt ccggaccgta ctgttcccaa cgaccactct
     8881 cttgccacaa atcggctggc tgaaccaccg gcatcgacac ctcgattgca ccggcgttgt
     8941 tcatctcttc acgcacgatg ttttcgactt ttttcagaac gcgcacgccg gtcggcagcc
     9001 aggtatataa cccggaggcc agcttgcgga tcatcccggc gcgcagcatc agctgatggc
     9061 tgatcacctc ggcgtcggca ggtgtctcct tgagagtgga gagcaggtat tggctagtac
     9121 gcatgttgtt acggttccag ttggaaggta gaacaggctc aaggcgagcc tgggacaaaa
     9181 aaagtgattt agtttaccag tgcaaaagaa atgtcaaaag agaagggcgt gaatttaacg
     9241 cggttccagc gcaaagactt caaaacctgc gtcggtgacg cgccagcgaa cgttaaaatc
     9301 atgtagccag acggcatagg ttttgcccgt ttcctcacct ttacgatagg ccgggcgcgg
     9361 gtcctgcgcc agtacttcgc ggataaacag cgttaactgc ggataacgct tctccagcgt
     9421 caaaagctgc ttttcgacct ctgcggtaaa actcaccgcc atctctgcag ctggcgcact
     9481 ttgcgcatag ctggcactgg catcgggaag cgattcggca aaggggagat acggtttgat
     9541 atccactacc ggcgtaccat cgaccagatc cagactgccg agcttcagaa tcacgctgtc
     9601 tttatggcaa acaacctctt tcagctctac cagcgacata ccaattgggt tagggcggaa
     9661 agtagagcgt gttgcgaaaa cccccattct ggcgttaccg ccgaggcgcg gtggacgcac
     9721 agtcggacgc cagccgcctt ccatcgtttg atgaaagacg aaaaggatcc ataaatggct
     9781 gaacgcttcc aggccgcgaa cggcgtcggc ctggttgtag ggagcaatga gatgcagttc
     9841 gccgttggcg ctttttacca gaccaggctg gcgcggaacg gcgaattttt ctttataggg
     9901 cgagcgaata acgcctattt gctcgaactg gaaactgctc atttcgccgt aatgttaagc
     9961 gcagagccga tacatacagc ctgacgatag cagcctagcg taccgctggt gacttcgcag
    10021 ctatgcagta ataccgcatt ggctttcatt ttagaggcgt tgatttgcat ccgcttacgc
    10081 gcggttggaa tgctcggcgg agagtcctga ttagaggcct ggcaagagtc gccactgact
    10141 tcaccgagat cgcggaacgg tttgccgact aattcttctg cattggtata aattcggacc
    10201 ggcgtggcgc gcggcgcttt cggttttgca ggctccgctt tcggctgggg tgcagtgctt
    10261 tgaacgggtt cgacagggga tctgcttaac atggaacagc cgcttagcat gagtgctact
    10321 aaacagatcg gtaaagcacg catagtattt cctcaatgta tgatcaaaac gtcaatattg
    10381 aatcaggagc ttgtaaaaat gacaagacgg gcaagcgccc gtcctgaatg atattacaaa
    10441 ttgtggaaac agcctaaaaa ttaccagcct ttaacagcgc cgccgttaaa cactttgttt
    10501 gctgcttcgt aaacttcatc agactgataa gcctggacga atttcttcac gttctcggcg
    10561 tctttgttat cttcacgcgt tacgatcagg ttcacgtacg gggagtcttt atcttcgaca
    10621 aagataccgt ctttcgctgg agtcaggcca atctggctgg cataggtggt attgataact
    10681 gccagagcga tttgcgcgtc gtccagagat cgaggcagct gcggtgcttc cagctcaaca
    10741 attttcagat tttttgggtt ctcaacaaca tcaagaacgg tcggcagcag gccaacgcca
    10801 tctttcagtt tgatcaagcc cactttttgc agcagcagca gtgaacgacc aagattagtt
    10861 ggatcgtttg gcacggcaac ctgcgaacca tcctgcagtt catccagtga tttgattttc
    10921 ttggagtaac cagcaatcgg ataaacaaaa gtgttgccta ctgcgaccag tttgtagcca
    10981 cgatctttca gttgctgatc aaggtacggt ttatgctgga aggcgttggc gtcgatatcg
    11041 cctttgctca atgcttcgtt tggcagaaca tagtcgttga aggttaccag ctcaacgtcc
    11101 aggccatatt tgtctttcgc aactttctgc gcgacttctg ctacctgctg ttcggcacca
    11161 acaatcacgc cgactttaat gtggtttgga tctttttcat cctgaccgca gcctaccagt
    11221 gccagtgatc caatcagggc tcccactgcc gcaaaggttt tgaatttgaa cgccatacct
    11281 tattccttct tcaattattt atgttgtgtt gaacgttact tgcgagtgac agcccggacg
    11341 atgcggtcgc ctgcgaactg aattaaataa accagaatga ccagcaatac cagtaccgta
    11401 ttcatcaccg tcgcgttgta gccgatgtag ccatactgat agccaatctg acctaaacca
    11461 ccggcaccga ctgcaccacc catcgcggaa taaccgacca gggtaatcag ggtgatagtt
    11521 gccgcattca ccagacccgg caacgcttcc ggtaacagca ctttacggac gatctgcatt
    11581 ggcgtggcac ccattgcgcg ggaagcttca attaacccgg ttgggatctc cagcagagcg
    11641 ttctcgacca tacgggcaat aaacggtgct gcaccaacgg ttaacggaac aattgctgcc
    11701 tgcaaaccaa tcgatgtacc gacaataacg cgggtaaacg gaatcatcca taccagcaag
    11761 ataatgaacg ggatggaacg gaaaatgttc acaatcgcag aaacggtacg atacagcttc
    11821 gcgttagcaa taatttgccc cggacgcgtg acataaagca gaacgccaac cggcagacca
    11881 atcacaaagc caaaaaatcc ggatacgaag gtcattgcca gcgtttccca tacgccacga
    11941 accagcagcc acatcatcgg ctcagacata acccagtacc tctactttta catggtgttc
    12001 ctgcagccag gcaatggcgg cttgcgtatc ttgttgtgtg ccgtgcattt cagtcagcat
    12061 gatgccgaat ttaacgccac cggcgtagtc catctgcgcg ctaataatgt tgttgttgac
    12121 gttgaaacga cgcgcggttt cagaaagcag tggggcatcg accgattgac cggtaaactc
    12181 cagacgcagc atcggcacgc agtcagtaaa tggctccgct tgcagacgtt cctggtaatc
    12241 ttccgggata tccagatgca gggtcgactg aataaacttc tgcgccagcg gcgttttcgg
    12301 atgcgagaac acttcactta ccgtgtcctg ctcgatcagt tcgccattgc tgatgaccgc
    12361 cacgcaatca caaatacgct tcacaacgtc catttcgtgg gtgatcaaca gaatcgtcaa
    12421 acccagacgg cggttaatgt ctttcagcag ttcgagaata gaacgtgtcg ttgccgggtc
    12481 cagcgcgctg gtggcttcat cacacagcaa tactttggga ttgctggcta atgcacgggc
    12541 aatcgccaca cgttgtttct gcccaccgga aagattcgac gggtagctat catgcttatc
    12601 gccaagacca accaatgaca gcaattccgt cacgcgacgt ttgatctcgt ctttcggtgt
    12661 gttgtccagc tccagcggca gagccacgtt gccaaaaaca gtacgcgaag agagcaggtt
    12721 aaaatgctgg aaaatcatac caatctggcg gcgagctttg gtcaactcgg attctgacag
    12781 cgtggtcaat tcttggccat cgaccagcac gctaccctcg gttgggcgct ccagcaggtt
    12841 tacacaacgt ataagcgtac tcttacccgc gcctgaggca ccgataacgc cataaatttg
    12901 tccagctggc acatgcaggc tgacgttgtt caacgcctgg atggtgcggg tgccctggtg
    12961 gaacactttg gtgatattcg aaagttttat cattgattat ttattatcgt cattaagtta
    13021 gtcgtggcat ctcgaatgcc tgaaacgggc aacgccgtca atgaaatgga tgttaaggca
    13081 tccagacgtc taaatcaatc aggtttatgc gaagagcact ttcttgcagg tcgaaacatg
    13141 cgatactagc gtcccatgcc ttattaagga gctataaaag gtggcgaaga gcgtacccgc
    13201 aatttttctt gaccgtgatg gcaccattaa tgtcgatcac ggctatgtcc atgagatcga
    13261 caactttgaa tttatcgacg gtgttattga cgccatgcgc gagctaaaaa aaatgggctt
    13321 tgcgctggtg gtagtaacca accagtctgg cattgctcgc ggtaaattta ccgaagcaca
    13381 gtttgaaacg ctgaccgagt ggatggactg gtcgctggcg gaccgagatg tcgatctgga
    13441 tggtatctat tattgcccgc atcatccgca gggtagtgtt gaagagtttc gccaggtctg
    13501 cgattgccgc aaaccacatc cggggatgct tttgtcagca cgcgattatt tgcatattga
    13561 tatggccgct tcttatatgg tgggcgataa attagaagat atgcaggcag cggttgcggc
    13621 gaacgtggga acaaaagtgc tggtgcgtac gggtaaacct attacacctg aagcagaaaa
    13681 cgcggcagat tgggtgttaa atagcctggc agacctgccg caagcgataa aaaagcagca
    13741 aaaaccggcg caatgattaa aagatgagcg gttgaaataa aaatgcattt ttccgcttgt
    13801 cttcctgagc cgactcccta taatgcgcct ccatcgacac ggcggatgtg aatcacttca
    13861 cacaaacagc cggttcggtt gaagagaaaa aatcctgaaa ttcagggttg actctgaaag
    13921 aggaaagcgt aatatacgcc acctcgcgac agtgagctga aagctgcgtc gcaactgctc
    13981 tttaacaatt tatcagacaa tctgtgtggg cactcgaaga tacggattct taacgtcgca
    14041 agacgaaaaa tgaataccaa gtctcaagag tgaacacgta a
//
LOCUS       DAJTZL010000110        13903 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.110, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000110 DAJTZL010000000
VERSION     DAJTZL010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13903
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.110"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            242..1495
                     /gene="kch"
                     /locus_tag="OO544_002777"
     CDS             242..1495
                     /gene="kch"
                     /locus_tag="OO544_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HCR6768329.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESVQK"
     gene            complement(1550..1723)
                     /locus_tag="OO544_002778"
     CDS             complement(1550..1723)
                     /locus_tag="OO544_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HCR6768330.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            1866..3326
                     /gene="cls"
                     /locus_tag="OO544_002779"
     CDS             1866..3326
                     /gene="cls"
                     /locus_tag="OO544_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HCR6768331.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            3361..3690
                     /locus_tag="OO544_002780"
     CDS             3361..3690
                     /locus_tag="OO544_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HCR6768332.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDIFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(3743..4747)
                     /gene="oppF"
                     /locus_tag="OO544_002781"
     CDS             complement(3743..4747)
                     /gene="oppF"
                     /locus_tag="OO544_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HCR6768333.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATGGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPISALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTCCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(4744..5757)
                     /gene="oppD"
                     /locus_tag="OO544_002782"
     CDS             complement(4744..5757)
                     /gene="oppD"
                     /locus_tag="OO544_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppD"
                     /protein_id="HCR6768334.1"
                     /translation="MSVIETATVPLAQQQADVLLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            complement(5769..6677)
                     /gene="oppC"
                     /locus_tag="OO544_002783"
     CDS             complement(5769..6677)
                     /gene="oppC"
                     /locus_tag="OO544_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HCR6768335.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTSGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            complement(6692..7612)
                     /gene="oppB"
                     /locus_tag="OO544_002784"
     CDS             complement(6692..7612)
                     /gene="oppB"
                     /locus_tag="OO544_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HCR6768336.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIVALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            complement(7698..9329)
                     /gene="oppA"
                     /locus_tag="OO544_002785"
     CDS             complement(7698..9329)
                     /gene="oppA"
                     /locus_tag="OO544_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HCR6768337.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDSTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            complement(10067..10714)
                     /gene="ychE"
                     /locus_tag="OO544_002786"
     CDS             complement(10067..10714)
                     /gene="ychE"
                     /locus_tag="OO544_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HCR6768338.1"
                     /translation="MIQTFFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            11192..13867
                     /gene="adhE"
                     /locus_tag="OO544_002787"
                     /gene_synonym="adhC"
     CDS             11192..13867
                     /gene="adhE"
                     /locus_tag="OO544_002787"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HCR6768339.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
BASE COUNT         3658 a         3437 c         3230 g         3578 t
ORIGIN      
        1 cttatagccg gtcagtgcat tcactaccac aagcagataa taactttatc tgtatttctt
       61 tatatgatgt gagcatattc aggaaaaatg acctttcctg aggtacagtt gctcgctcaa
      121 atcctttgat tactgcggat aaagtcatct actattgttt aacacattca cgcgctgcat
      181 gtatgttcag aaaatgtgca cgctaagata attaaacaaa attgtggtta cagaggtatc
      241 agtgagtcac tgggctacat tcaaacaaac agcaacaaat ttatgggtga cccttcggca
      301 cgacattctc gcgctggccg tctttttaaa tggattgctt atttttaaaa caatctatgg
      361 tatgtcggtc aatttgcttg atattttcca tatcaaagca ttttcagagt tggatctctc
      421 cttgctggca aacgccccac tatttatgct cggcgtcttt cttgtcctga actccattgg
      481 cttactgttc cgggcaaagc tcgcatgggc aatcagtatc attttgttgt tgatagcgct
      541 aatttacacc ctgcattttt atccctggct gaaatttagt attggatttt gcatttttac
      601 gctggtgttt ttgctgatac tgcgcaaaga cttctcccac agtagcgccg cagccgggac
      661 aatttttgca tttattagtt tcacgacatt actgttttac tcaacctacg gtgcgcttta
      721 tttaagcgaa ggttttaatc cgcgaataga aagtttgatg accgcgttct atttttcgat
      781 agaaaccatg tcaaccgtcg gctatggcga tattgtccct gtttctgaat cagcacgatt
      841 gttcactatt tcggtcatta tttccggcat taccgttttt gccacatcta tgacctcaat
      901 ttttggtccg cttatccgcg ggggattcaa caaacttgta aaaggaaaca atcatacaat
      961 gcatcgtaaa gatcatttta ttgtttgcgg acattcgatt ctcgccatca atacgatcct
     1021 gcaactgaat caacgcggac aaaacgtaac ggttatcagc aacttgcctg aagatgatat
     1081 caagcaactt gagcaacgct taggcgataa cgctgatgtt atccccggtg acagtaatga
     1141 cagttcagta ttaaagaaag cgggaatcga tcgttgccgg gccattctgg cgctgagtga
     1201 taacgatgca gataacgcgt ttgttgtact ctcggcaaaa gatatgagca gtgatgtcaa
     1261 aacagttctc gccgtcagtg atagcaaaaa cctgaataag attaagatgg tacatccgga
     1321 tatcattctc tcaccgcaac tgtttggcag cgaaattctg gcgcgagttt taaatggtga
     1381 agagattaat aatgatatgc tcgtttcaat gttgttgaac tccggtcatg gcattttcag
     1441 cgataacgat gaacaagaaa cgaaagctga cagtaaagaa tcagtgcaaa aatagagttt
     1501 aaagacgaaa gagtaaactt attgggcacc gcaatggtgc ccagtatatt tagatattgt
     1561 agatcgcaaa caataacgag ttacgctgtt tgtttagaat gcacttcctg atgctgtgga
     1621 tacgcatatt tcggcgcgat tgcccctcaa gccaacgcga tttacgtcgg ctagcctgac
     1681 gctgcatgcg ccagcgcccc acttccgttc tactccgctt catgttaact actctatgca
     1741 ataacagaat ggtcattata cccccacccc cgacgctgac cagtgctttt cccgcgtttt
     1801 tatttgccag atgaatccat atgcgtaaac tcataacaat gcgctttcaa aaggatttct
     1861 aatctatgac aaccgtttat acgttggtga gttggttggc cattctggga tactggttgc
     1921 tcattgcagg cgtaacttta cgcattctaa tgaaacgacg cgcagttccc tccgcgatgg
     1981 cctggctgtt gattatttac attctgccgt tagtcggaat tattgcctat cttgccgttg
     2041 gcgagctcca tttaggcaaa cgccgcgctg agcgcgccag agcgatgtgg ccttccaccg
     2101 caaaatggct taacgacctt aaagcctgta agcatatctt cgccgaagaa aatagcagtg
     2161 tcgctgcgcc attattcaag ctttgcgagc gtcgtcaggg gatcgctggg gtcaaaggga
     2221 atcagctaca actgatgacc gagtcagatg atgtgatgca ggcgttaatc cgcgacatcc
     2281 agctcgcgcg ccataatatt gagatggtgt tttatatctg gcagcccggc ggcatggcgg
     2341 accaggtggc tgaatcatta atggcggctg cacggcgtgg tattcattgc cgattgatgc
     2401 tcgactccgc cgggagtgtg gcttttttcc gcagcccgtg gcccgagcta atgcgtaatg
     2461 ccggtattga agtggtcgaa gccttaaagg tcaatctgat gcgtgtgttt ttacgccgta
     2521 tggacctgcg ccaacatcgc aagatgatca tgatcgataa ttacatcgcg tacaccggca
     2581 gcatgaatat ggtcgatcct cgctacttca aacaagatgc gggcgtaggg caatggattg
     2641 atctgatggc gcgtatggaa ggccccatcg ccaccgcgat ggggattatt tattcctgcg
     2701 actgggagat tgaaaccgga aaacgtattc tgccgccacc accagatgtc aatattatgc
     2761 cgtttgaaca ggccagcggt cacaccattc acacaattgc ttctggcccc ggctttccgg
     2821 aagatctcat tcaccaggca ttattgactg cggcttattc ggcgcgtgaa tatttgatca
     2881 tgacaacgcc ctactttgtg ccaagcgatg atttacttca tgcgatttgc acggcggcgc
     2941 agcgcggggt ggatgtcagt attatccttc cgcgaaaaaa tgactcgatg ctggtcggct
     3001 gggccagtcg cgcattcttt acggaactgc ttgctgctgg ggttaaaatt tatcagtttg
     3061 aaggcgggtt actgcatacc aagagcgtgc tggtcgatgg cgaactaagt ctggttggca
     3121 ccgttaacct tgatatgcgt agtctgtggc tgaatttcga gattaccctg gcaatcgacg
     3181 ataaaggttt tggtgcagac cttgccgccg ttcaggacga ttatatttcg cgttcacgtc
     3241 tgctcgatgc ccgtttatgg ctaaaacgtc cattatggca acgtgtcgcc gagcgactgt
     3301 tttacttctt cagcccgttg ctgtaaaacg tcgccaacag aggttaaaca ggtagtgact
     3361 atggatatgg atctaaacaa tcgcctgact gaagatgaaa cgcttgagca ggcctacgat
     3421 atttttctcg aactggctgc cgacaatctc gacccagctg atgttctgct gttcaatctt
     3481 cagtttgaag agcgcggcgg cgcggagtta ttcgatccgg cggaagactg gcaggaacat
     3541 gttgatttcg atttgaaccc ggactttttt gccgaagtgg tgattggtct ggcggacagt
     3601 gaggatggtg aaatcaacga tattttcgcc cgcattctgt tatgccgcga aaaagatcac
     3661 aaactttgcc atatcatctg gcgggaataa ataatcgctt tcggcgtgaa aaagggctga
     3721 caactgtcag cccttattgt gtttaaagcg gatcgacttt caggcaagaa acggcgtgtc
     3781 tgaaactccc ttccagcaca ggacgtgttt tggcgcactc cggaccggca atcgggcaac
     3841 aggtacggaa aacacaaccg gaaggcgggt tgatcggcga cggtaattcc ccttccagta
     3901 actggatggt tttgttcttc tccagatccg gatcaggtat ggggactgcc gacatcaatg
     3961 ccctggtgta aggatgtagt ggattgtggt agacctcatc ataggtcccc agttctaccg
     4021 catggccgag atacatcacc aacacacgat cggaaatgtg ttttaccacg gccaggtcat
     4081 gagcgataaa aattaatgac aatcccatct cacgttgcag ctgctggagc aggttgacca
     4141 cctgcgcctg aattgacacg tccagcgccg aaatcggctc atcgcagata atcagcttcg
     4201 gttcaagaat aagagcacga gcaatcccga tacgctggca ctgcccaccg gagaactcat
     4261 gcggatagcg gttaatcagg ttaggcaata acccgacttt cagcatcatc gccttcacgc
     4321 gctcgcgaac ttcctggcgt gacattttcg gatgataagt acgcagtggt tcagcgatga
     4381 tctcgccgat ggtcatacgc gggtttagcg atgccaacgg atcctggaaa atcatctgaa
     4441 tatcactgcg aacggcacgc cattcatcgg gcttcatgcc cagcaactct ttacctaacc
     4501 aggcaacatg accgccggtc gctttaacca ggccaatgat ggcgcgagca aaggtggact
     4561 taccgcatcc cgattcccct accacaccta atgtttcccc ttcatacagg cgaagcgtga
     4621 caccatcgac ggctttgaga gttttcggcg gttgccagaa ccactgtttg ccatctttga
     4681 tttcaaagtg cactttcaga tcggcgattt cgaggaggac ttttcttcct tcggttacag
     4741 cattcataac agttcctcca ccggtttaaa gcaagcacgc agacggccag gcgtaaactc
     4801 ttccagcggc ggtgcgctac tacaaatttc catcgcatgc ggacaacgtg gctggaacgg
     4861 gcaacctttc ggtaatcgca gcaggtttgg cggattacca gggatggtca acattgtttc
     4921 accttccgca tcgagacgcg gcaccgcgtt gagcaaaccg atagaataag gatgaacggg
     4981 ttgataaaag acatcgcgcg cgttgccata ttccatcgtg cgcccggcgt acattaccag
     5041 cactttgtcg cagatccccg ccaccacgcc aagatcgtgg gtaatcataa tgatggcggt
     5101 attaaattcc cgcttcagtt catttaatag cgtcatgatc tgcgcctgta cggtgacgtc
     5161 cagcgcagta gtgggttcat ccgcaatcag cagcttaggt cgacatagca atgccatcgc
     5221 aatcataact cgctgacgca tgccaccaga aaattcgtgc gggtacattt tcatgcgttt
     5281 acgtgcttcc ggcattttta ccgcatcgag catccgcacc gactcttcaa acgcctcagc
     5341 tttgctcatg ttcttatgca gcatcagcac ttccatcaac tgctcaccga cgcgcatata
     5401 gggattcaac gaagtcattg ggtcctgaaa aatcattgag atttgttcag cgcgcagttt
     5461 attgagttca cgctctggca aattgaggat ttcacgccca ttgaaggttg ccgatccgcc
     5521 aatacgtccg ttggcagcca acaggcccat caacgcaaat gcagtttgcg atttacccga
     5581 accagactca cctacaatgc ccagcgtttc tccggcacgt agagaaaaat tcaaatcatt
     5641 gaccgccgtg acatcgccgt ccggggtact aaaggtgaca cgcaaatctt tcacgttcag
     5701 cagtacgtca gcctgttgtt gtgcgagcgg cacagttgca gtttcaatta cgctcatggc
     5761 tgcactcctt aacgatcttt cgggtcgaga gcatcacgca agccatcgcc gataaagttg
     5821 aaacaaaaca gcgtcaccac gaggaatccc gctgggaaca acaataacca tggagagact
     5881 tccatcgagt tcgcgccatc actcagcaat gcaccccagc tgcttaacgg ctcttgcgta
     5941 cccaacccca ggaagctaag gaaagattca aagaggatca tgctgggcac cagaagtgat
     6001 gcgtagacca ccaccacacc gagtacattc ggcacaatgt ggcgaataac aatgcccgat
     6061 gtcgatacac cgccaacttg tgccgcctca ataaactctt tgcgcttcag actcagggtt
     6121 tgtccacgca caatacgagc catatccagc caggaaacca tgccaatcgc cacgaaaatc
     6181 agcaggatgt tttgaccgaa aaaggtcaca agcaaaatga cgaagaacat gaatgggaag
     6241 gagttgagga tttccagcag acgcatcatt accgaatcca ctttaccgcc cagataaccg
     6301 gaaagcgaac cgtaaagtgt ccccacgacc actgccacca gtgccgcagc aacaccgacc
     6361 atgagtgaga tacgcccgcc aatcgcaacg cgcacaagca ggtcgcgacc ggatgagtca
     6421 gtaccaaagt agtgaccgga ctccatatcc ggggcgctgg acatcatcgc ccagtcagta
     6481 tcgtcatagg caaactgcga aagcatcggt gccaggatta caaataacgc gatcagcacc
     6541 agcactatca gactggcaac cgccgcacgg ttatgcataa aacgtcgacg tgcgtcctgc
     6601 cacaagctgc gcccttcgac ctccagcttt tcactgaaat tttccagcgt ctcgctgttt
     6661 ttcttactta acatcatcgc gagctccagt atcagtaacg gattttcggg tcgataaccg
     6721 catatagcac atcgacaatg gcattaaaca aaatggttaa agcaccaacc aggatggtca
     6781 ggcttaacac taaggaatag tcacggttca atgcgccatt aacgaacagt tgtccaatcc
     6841 ccggcaaacc ataaatggtt tcgataacca tagaaccggt aataatgccg acaaatgcgg
     6901 ggcccatata agagagcacg ggtaacagag caggttttaa tgcgtggcgt aaaatgatcc
     6961 gccgcatagg taaccctttc gcccgggcag tacgaataaa gttggagtgt aatacttcaa
     7021 tcatagagcc tcgggtaata cgcgcaatac tggcgatata agcgagtgac aacgccacca
     7081 tcggcaatat catgaattta agcgccccac cattccagcc accgcccggc agccaatgca
     7141 aaatgatcgc aaatatcatg actaataatg gcgcaaccac aaaactgggg ataacaaccc
     7201 cggtcattgc cagccccatc acggtatagt cccatttggt gttttgtttt aatgcgacaa
     7261 taacgccagc actaacaccc agtattaccg caaggaaaaa tgcggcggct cccagtttgg
     7321 cagaaacggg aaaactggat gcaaccaggt cattgaccga ataatcttta tatttaaacg
     7381 atggaccgaa atcaccgtgc gccagttgtt tcaggtagct gaaatactgt gtcatgatcg
     7441 gatcattaag atgatatttc gcttcgatat tggccatcac ttccggcggt aaagtacgtt
     7501 cgccggtaaa agggcttccc ggcgcgaggc gcatcataaa gaacgaaata gtaataagaa
     7561 taaatagcgt cggaatcgct tccagacagc gacgtagaat aaattttaac attgccctac
     7621 cttctggcct gtgccttctg taatgcgata aaaatcagac accgtggagc aggacactcc
     7681 tgccccacgt attgccatta gtgcttcaca atgtacatat tccgggtgta ggtattatcc
     7741 agcggatctt tgccggtata gccaccaacc cacggtttca ccagacgcgc attcacgtag
     7801 taataaacag gaacaatggc cgaatcctta tccagctgtt gttctgcttt agtgtacaga
     7861 gctgtgcgct gcgcctcgtc agtcactttc agcgtttccg ccataatgct gtcaaaggcc
     7921 gggctcttat aatgcgcggt attcatcgag ctgttcgaca gcatggtgtt caggaaggaa
     7981 gttggttcgt tgtagtctgc acaccagcct gcacgggcca catcaaaagt gccctggtga
     8041 cgggtgtcga ggaacgtttt ccactcctgg ttaaccagtt tgacgtttac accaatgttt
     8101 ttcttccaca atgaagaggc agcaatcgcc agctttttat gcagatcaga ggtgttatac
     8161 agcaggttga tggtcaacgg tttgtctgcg gtataacccg cttcagccag cagttttttc
     8221 gcttcttcgt tacgtttttc ctggctccag ccaaaccatt ctggctgagt caattttgcg
     8281 ccatcagtat acggtggagt gtaaccatag gcgggcatgt cgccctgcgc tttcacttta
     8341 ttaacaatga tatcgcggtc catacctagt ttcagtgcgg tacgcacacg cacatcgttg
     8401 aatggcggtt tctggttgtt aatttcgtaa tagtaagtgc acaggtatgg atcaacgtga
     8461 acttcgtccg ggatctcttt tttcagcttc tggaacaatt cgatcggcat gttgttatac
     8521 gtcatgtcga tttcaccact acggtagcgg ttgacatcgg taacttcaga agcaataggc
     8581 aaataggtta cctggttaat aacggttttc gcgttgttcc agtaggtcgg gctgcgttca
     8641 agaacgattc gttcgtttac gacccaatct tttaaggtat aggcaccgtt ggtgacgata
     8701 ttaccaggct gggtccattt ttcgccgaat ttctcgatag cggcttttgg caccggtgaa
     8761 gttgatgggt gaacaagtaa tttatagaag tacggaacgg gttcacttaa ggtgacttct
     8821 aatgtgtgat catcaatagc tttcacgccg agatcggtaa tcggtttttt cccttcaaga
     8881 atttcatcaa taccggcgat atgcccatat tgcagataac tggcatacgg agaagcagtg
     8941 ttcggatcaa cagaacgttg ccagctatac acaaagtctt gtgctgtgac tggcgtgcta
     9001 tcagaccatt tcgcatcttt acgcaaatgg aaggtccaga ctttcgcgtc tttattatcc
     9061 caggattcag cgacgccagg tgctggatga ccgtcaagat cgctgaccag taagccttca
     9121 aacaggtctc ggctgatatt agactccgga acaccttcaa ttttgtgcgg atctaatgac
     9181 tgaacttctg aaccattgtt acgtaccagt gtttgttttt ccgccagtgt gacgcctgcg
     9241 ggtacatcag ctgccagcgc gacattccct gccattagcg cagccagaac gccagctgct
     9301 actaaacttc tcttggtgat gttggtcatt gttttttgga ctccctcatt ataattactg
     9361 gtattaacca gcatgtgtaa tcccggattg ggaccctgca caggctcgga gactttctgc
     9421 tgtcaggtta ctttatcgtt acgtattact atcaccgact ttatttattc tggtgattca
     9481 gacgatcacc ttatgtcgat tcgtactgtg tctcccctgt cgagacacat tattctttcg
     9541 cgacgctctt taattgttaa ttattctcat taccaataaa gaatcgtctg gcggttgccc
     9601 ggaaagtacc aaatgcggtt cttactcgcc aatacatttt gcaaaattgt taagcaattc
     9661 tcttttattg accgtatcag aatctatagc ttgcatccat gccttaacaa acatctttca
     9721 attcaatcaa atagagattg gctaagcatg tctatctttt ctgattacag ttccagtagt
     9781 gaaatgcaca ataatttaac aattgattat tatttagcac tttcctctac caaaggcagt
     9841 ggaattacta atatcatttc aattattctt cagcaagccc aggattatga tgtggcgaaa
     9901 ataacatgat ggtgaggaga agcagttaag gaaatcctta taactaactg taatttgaga
     9961 agaatatttt tacctattcc ttttgctttt gataactgcg cgggaacaaa gtaaaaataa
    10021 tgacgaagta aacggagcat ggcagctccg tttcatttga aaggaattaa ttaagcaggc
    10081 cggggaaaat ccccttaata ccagtaacga taaattcaat ccccaatgcc atcagcaata
    10141 gccccataat acgcgtaatc acgttgatgc cggtctggcg taaaacccgt accagccacg
    10201 gtgccatgcg gaacaatccc cagcaacata aagcgaacaa tgcaatagcc acaaagaaac
    10261 caaacagata gctaatgctg tgataacgcg taccccagac gatggtagaa ctgatcgccc
    10321 ctggccccgc catcaacggc aacgccaatg gcaccacacc aatgctttca cgtaccgcgg
    10381 tttctgattt ttcttgcttg ttctgtttat cctcgccaag cttgccgctg atcatcgaca
    10441 tcgctatcgt caccaccagg atgccccctg cgatacggaa cgaatcaatt gatataccaa
    10501 aaagttgcag aatcgtgtcg ccgagaaaaa gcgagatcca caagataatg gccacagaca
    10561 ggttggctgt aaggttagtt ttgtttcgcg ctgccgctgt ctgataactg gtcatgctga
    10621 taaagacggg aataatccct accgggttga ccagcgcaaa taacccgatg aaaaatttga
    10681 agtaaacggg aaaatcaaaa aaggtctgaa tcacggttag ctccgaagca aaagccggat
    10741 aatgttagcc ataaattaag gttgaaaaga cgcgctgaca atacgccttt tgacagcatt
    10801 tttcacctcc taactactta aaattgctat cattcgttat tgttatctag ttgtgcaaaa
    10861 catgctaatg tagccaccaa atcatactac aatttattaa ctgttagcta taatggcgaa
    10921 aagcgatgct gaaaggtgtc agcttagcaa aaatttgatt tggatcacgt aatcagtacc
    10981 cagaagtgag taatcttgct tacgccacct agaagtgacg cattagagat aataactcta
    11041 atgtttaaac tcttttagta aatcacagtg agtgtgagcg cgagtaagct tttgattttc
    11101 ataggttaag caaatcatca ccgcactgac tatactctcg tattcgagca gatgatttac
    11161 taaaaaagtt taacattatc aggagagcat tatggctgtt actaatgtcg ctgaacttaa
    11221 cgcactcgta gagcgtgtaa aaaaagccca gcgtgaatat gccagtttca ctcaagagca
    11281 agtagacaaa atcttccgcg ccgccgctct ggctgctgca gatgctcgaa tcccactcgc
    11341 gaaaatggcc gttgccgaat ccggcatggg tatcgtcgaa gataaagtga tcaaaaacca
    11401 ctttgcttct gaatatatct acaacgccta taaagatgaa aaaacctgtg gtgttctgtc
    11461 tgaagacgac acttttggta ccatcactat cgctgaacca atcggtatta tttgcggtat
    11521 cgttccgacc actaacccga cttcaactgc tatcttcaaa tcgctgatca gtctgaagac
    11581 ccgtaacgcc attatcttct ccccgcaccc gcgtgcaaaa gatgccacca acaaagcggc
    11641 tgatatcgtt ctgcaggctg ctatcgctgc cggtgctccg aaagatctga tcggctggat
    11701 cgatcaacct tctgttgaac tgtctaacgc actgatgcac cacccagaca tcaacctgat
    11761 cctcgcgact ggtggtccgg gcatggttaa agccgcatac agctccggta aaccagctat
    11821 cggtgtaggc gcgggcaaca ctccagttgt tatcgatgaa actgctgata tcaaacgtgc
    11881 agttgcatct gtactgatgt ccaaaacctt cgacaacggc gtaatctgtg cttctgaaca
    11941 gtctgttgtt gttgttgact ctgtttatga cgctgtacgt gaacgttttg caacccacgg
    12001 cggctatctg ttgcagggta aagagctgaa agctgttcag gatgttatcc tgaaaaacgg
    12061 tgcgctgaac gcggctatcg ttggtcagcc agcctataaa attgctgaac tggcaggctt
    12121 ctctgtacca gaaaacacca agattctgat cggtgaagtg accgttgttg atgaaagcga
    12181 accgttcgca catgaaaaac tgtccccgac tctggcaatg taccgcgcta aagatttcga
    12241 agacgcggta gaaaaagcag agaaactggt tgctatgggc ggtatcggtc atacctcttg
    12301 cctgtacact gaccaggata accaaccggc tcgcgtttct tacttcggtc agaaaatgaa
    12361 aacggctcgt atcctgatta acaccccagc gtctcagggt ggtatcggtg acctgtataa
    12421 cttcaaactc gcaccttccc tgactctggg ttgtggttcc tgggggggta actccatctc
    12481 tgaaaacgtt ggtccgaaac acctgatcaa caagaaaacc gttgctaagc gagctgaaaa
    12541 catgttgtgg cacaaacttc cgaaatctat ctacttccgc cgtggctccc tgccaatcgc
    12601 gctggatgaa gtgattactg atggccacaa acgtgcgctc atcgtgactg accgcttcct
    12661 gttcaacaat ggttatgctg atcagatcac ttccgtactg aaagcagcag gcgttgaaac
    12721 tgaagtcttc ttcgaagtag aagcggaccc gaccctgagc atcgttcgta aaggtgcaga
    12781 actggcaaac tccttcaaac cagacgtgat tatcgcgctg ggtggtggtt ccccgatgga
    12841 cgccgcgaag attatgtggg ttatgtacga acatccggaa actcacttcg aagagctggc
    12901 gctgcgcttt atggatatcc gtaaacgtat ctacaagttc ccgaaaatgg gcgtgaaagc
    12961 gaaaatgatc gctgtcacca ccacttctgg tacaggttct gaagtcactc cgtttgcggt
    13021 tgtaactgac gacgctactg gtcagaaata tccgctggca gactatgcgc tgactccgga
    13081 tatggcgatt gtcgacgcca acctggttat ggacatgccg aagtccctgt gtgctttcgg
    13141 tggtctggac gcagtaactc acgccatgga agcttatgtt tctgtactgg catctgagtt
    13201 ctctgatggt caggctctgc aggcactgaa actgctgaaa gaatatctgc cagcgtccta
    13261 ccacgaaggg tctaaaaatc cggtagcgcg tgaacgtgtt cacagtgcag caactatcgc
    13321 gggtatcgca tttgcgaacg ccttcctggg tgtatgtcac tcaatggcgc acaaactggg
    13381 ttcccagttc catattccgc acggtctggc aaacgccctg ctgatttgta acgttattcg
    13441 ctacaatgcg aacgacaacc cgaccaagca gactgcattc agccagtatg accgtccgca
    13501 ggctcgccgt cgttatgctg aaattgccga ccacttgggt ctgagcgcac cgggcgaccg
    13561 tactgctgct aagatcgaga aactgctggc atggctggaa acgctgaaag ctgaactggg
    13621 tattccgaaa tctatccgtg aagctggcgt tcaggaagca gacttcctgg cgaacgtgga
    13681 taaactgtct gaagatgcat tcgatgacca gtgcaccggc gctaacccgc gttacccgct
    13741 gatctccgag ctgaaacaga tcctgctgga tacctactac ggtcgtgatt atgtagaagg
    13801 tgaaactgca gcgaaaaaag aagccgctcc ggctaaagct gagaaaaaag cgaaaaaatc
    13861 cgcttaatca gtagcgctgt ctggcaatat aaacggcccc ttc
//
LOCUS       DAJTZL010000111        13836 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.111, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000111 DAJTZL010000000
VERSION     DAJTZL010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13836
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.111"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(30..788)
                     /gene="dsbG"
                     /locus_tag="OO544_002788"
     CDS             complement(30..788)
                     /gene="dsbG"
                     /locus_tag="OO544_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HCR6768340.1"
                     /translation="MLKKILLLALLPAIAFAEELPSPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWILDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGGKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGVMTPTY"
     gene            1160..1723
                     /gene="ahpC"
                     /locus_tag="OO544_002789"
     CDS             1160..1723
                     /gene="ahpC"
                     /locus_tag="OO544_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HCR6768341.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            1894..3459
                     /gene="ahpF"
                     /locus_tag="OO544_002790"
     CDS             1894..3459
                     /gene="ahpF"
                     /locus_tag="OO544_002790"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HCR6768342.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(3580..4008)
                     /gene="uspG"
                     /locus_tag="OO544_002791"
     CDS             complement(3580..4008)
                     /gene="uspG"
                     /locus_tag="OO544_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HCR6768343.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVIR"
     gene            4229..5467
                     /locus_tag="OO544_002792"
     CDS             4229..5467
                     /locus_tag="OO544_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent formaldehyde
                     dehydrogenase"
                     /protein_id="HCR6768344.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITATAICGSDLHL
                     YRGKIPQVKHGDILGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRMQQYA
                     ACENTNAGKGAALNKKQIPAPAALFGYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNAQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLRFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGCSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKGLTFKMGQT
                     HVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAARGYEIFEKREEECRKVILVPGAQS
                     AEAAQKAVSGLVNAMPGGTI"
     gene            complement(5698..6108)
                     /gene="rnk"
                     /locus_tag="OO544_002793"
     CDS             complement(5698..6108)
                     /gene="rnk"
                     /locus_tag="OO544_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HCR6768345.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            complement(6338..7144)
                     /gene="rna"
                     /locus_tag="OO544_002794"
     CDS             complement(6338..7144)
                     /gene="rna"
                     /locus_tag="OO544_002794"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HCR6768346.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEAHASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKNFVIDKVGY"
     gene            complement(7258..8721)
                     /gene="citT"
                     /locus_tag="OO544_002795"
     CDS             complement(7258..8721)
                     /gene="citT"
                     /locus_tag="OO544_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HCR6768347.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLSVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            complement(8772..9650)
                     /gene="citG"
                     /locus_tag="OO544_002796"
     CDS             complement(8772..9650)
                     /gene="citG"
                     /locus_tag="OO544_002796"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HCR6768348.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEIAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSQLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMAINGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            complement(9625..10176)
                     /gene="citX"
                     /locus_tag="OO544_002797"
     CDS             complement(9625..10176)
                     /gene="citX"
                     /locus_tag="OO544_002797"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HCR6768349.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHVWLKRHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALAAKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            complement(10180..11712)
                     /gene="citF"
                     /locus_tag="OO544_002798"
     CDS             complement(10180..11712)
                     /gene="citF"
                     /locus_tag="OO544_002798"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HCR6768350.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDR
                     KLCANQEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            complement(11723..12631)
                     /gene="citE"
                     /locus_tag="OO544_002799"
     CDS             complement(11723..12631)
                     /gene="citE"
                     /locus_tag="OO544_002799"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HCR6768351.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            complement(12628..12924)
                     /gene="citD"
                     /locus_tag="OO544_002800"
     CDS             complement(12628..12924)
                     /gene="citD"
                     /locus_tag="OO544_002800"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HCR6768352.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            complement(12939..>13836)
                     /gene="citC"
                     /locus_tag="OO544_002801"
     CDS             complement(12939..>13836)
                     /gene="citC"
                     /locus_tag="OO544_002801"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HCR6768353.1"
                     /translation="AGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTK
                     TEYEALFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANP
                     FTNGHRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEY
                     IISRATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYN
                     QDMRYWLETPTISAPPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTL
                     HYLQNLLEHSRQDAAARQKTPA"
BASE COUNT         3400 a         3504 c         3678 g         3254 t
ORIGIN      
        1 atcgggcatt atctgaacat aaaacactat caataagttg gagtcattac ccccataatg
       61 atattaagcg ttttctgatc gggcaacccc acggcctgtt gcagcgtatt ttccttactc
      121 atgtaataga tagccggcgt gacatttgcc cccagatcgt ccatcagttt ctcattgtca
      181 cttaacactt tcatttgctc tgtacttacg tttgcaggca cgtttagctt aagcttgcca
      241 ccagaggctt catattgttg ccaggttttt gcgggatctt tggaggcaag aattgccgct
      301 gctgtcgccg ggctttctgg cttgataacc ccaaccaaca atgttcttaa ttgcactttg
      361 ccagaatcta cccacgggcg cgcctgctgc cagaactgtt tacaatatgg gcagaacgga
      421 tcggcgaaga cgtagacaat gaccggcgca tcttttttac cgtcgaggat ccagtgggat
      481 tgttccatcc gttgccacat ttcgcgtccg gctggtgcgt aaatttcttt ttcgataagt
      541 gtgttactca ggttttcacc tttctcgttg tacatgtaac cagagatagc gtgcttacca
      601 tcgggagtca ggtagatggt gacgcccata tcctgatact ttccgagata acctttcatt
      661 cctccggggg catcgaacgt tttgatgatt gtaatgccct gtttttcaat cgcttttact
      721 ggggaaggaa gttcctctgc gaaggcgatt gcaggaagca gagccagtaa aagtatcttt
      781 tttaacatta atttgtcctt ttcagtcagt gcaaaagtcg agtaaaaggc ataacctatc
      841 actgtcatag gtaagagctt agatcaggtg attgcccttt gtttatgagg gtgttgtaat
      901 ccatgtcgtt gttgcatttg taagggcaac acctcagcct gcaggcaggc gctgaagata
      961 ccaaagggta gttcagatta cacggtcacc tggaaagggg gccattttac tttttatcgc
     1021 cgctggcggt gcaaagttca caaagttgtc ttacgaaggt tgtaaggtaa aacttatcga
     1081 tttgataatg gaaacgcatt agccgaatcg gcaaaaattg gttaccttac atctcatcga
     1141 aaacacggag gaagtataga tgtccttgat taacaccaaa attaaacctt ttaaaaacca
     1201 ggcattcaaa aacggcgaat tcatcgaaat caccgaaaaa gataccgaag gccgctggag
     1261 cgtcttcttc ttctacccgg ctgactttac tttcgtatgc ccgaccgaac tgggtgacgt
     1321 tgctgaccac tacgaagaac tgcagaaact gggcgtagac gtatacgcag tatctaccga
     1381 tactcacttc acccacaaag catggcacag cagctctgaa accatcgcta aaatcaaata
     1441 tgcgatgatc ggcgacccga ctggcgccct gacccgtaac ttcgacaaca tgcgtgaaga
     1501 tgaaggtctg gctgaccgtg cgaccttcgt tgttgacccg cagggtatca tccaggcaat
     1561 cgaagttacc gctgaaggca ttggccgtga cgcgtctgat ctgctgcgta aaatcaaagc
     1621 agctcagtac gtagcttctc acccaggtga agtttgcccg gctaaatgga aagaaggtga
     1681 agcaactctg gctccgtctc tggacctggt tggtaaaatc taaatttcct tcgtctttcg
     1741 cgctaccagc tccggcgcga aatacttcgg aaattcacct aattcttcgg gtgctgcggc
     1801 acccgatttt cttccccgca ccatgatgca agctgcatcc aggtagccgt agaggccgct
     1861 tgcatgatga tgtttaaagc ccaggagata aacatgctcg acacaaatat gaaaactcaa
     1921 ctcaaggctt accttgagaa attgaccaag cctgttgagt taattgccac gctggatgac
     1981 agcgctaaat cggcagaaat caaggaactg ttggctgaaa tcgcagaact gtcagacaaa
     2041 gtcaccttta aagaagataa cagcttgccg gtgcgtaagc cgtctttcct gatcaccaac
     2101 ccaggttcca accaggggcc acgttttgca ggctccccgc tgggccacga gttcacctcg
     2161 ctggtactgg cgttgctgtg gaccggtggt catccgtcga aagaagcgca gtctctgctg
     2221 gagcagattc gccatattga cggtgatttt gaattcgaaa cctattactc gctctcttgc
     2281 cataactgcc cggacgtggt gcaggcgctg aacctgatga gcgtactgaa cccgcgcatc
     2341 aagcacactg caattgacgg cggcaccttc cagaacgaaa tcaccgatcg caacgtgatg
     2401 ggcgttccgg cagtgttcgt aaacgggaaa gagtttggtc agggccgcat gacgttgact
     2461 gaaatcgttg ccaaaattga tacgggcgcg gaaaaacgtg cggcagaaga gctgaacaag
     2521 cgtgatgctt atgacgtatt aatcgtcggt tccggcccgg cgggtgcagc ggcagcaatt
     2581 tactccgcac gtaaaggcat ccgtaccggt ctgatgggcg aacgttttgg tggtcagatc
     2641 ctcgataccg ttgatatcga aaactacatt tctgtaccga agactgaagg gcagaagctg
     2701 gcaggcgcac tgaaagttca cgttgatgaa tacgacgttg atgtgatcga cagccagagc
     2761 gccagcaaac tgatcccagc agcagttgaa ggtggtctgc atcagattga aacagcttct
     2821 ggcgcggtac tgaaagcacg cagcattatc gtggcgaccg gtgcaaaatg gcgcaacatg
     2881 aacgttccgg gcgaagatca gtatcgcacc aaaggcgtga cctactgccc gcactgcgac
     2941 ggcccgctgt ttaaaggtaa acgcgtagcg gttatcggcg gcggtaactc cggcgtggaa
     3001 gcggcaattg acctggcggg tatcgttgag cacgtaacgc tgctggaatt tgcgccagaa
     3061 atgaaagccg accaggttct gcaggacaaa ctgcgcagcc tgaaaaacgt cgacattatt
     3121 ctgaatgcgc aaaccacgga agtgaaaggc gacggcagca aagtcgttgg tctggaatat
     3181 cgagatcgtg tcagcggcga tattcacaac atcgaactgg ccggtatttt cgtgcagatt
     3241 ggtctgctgc cgaacaccaa ctggctcgaa ggcgcagtcg aacgtaaccg catgggtgag
     3301 attatcattg atgcgaaatg cgaaaccaac gtcaaaggcg tattcgcagc gggtgactgt
     3361 acgacggttc cgtacaagca gatcatcatc gccactggcg aaggtgccaa agcctctttg
     3421 agtgcttttg actacctgat tcgcaccaaa actgcataag aagaagtaag attcacctgc
     3481 aattgcttag ccgccggggt caaacctggc ggctttttta tggcattaaa aagccctgcc
     3541 gggatgttcc aggcagggcg gcataaatac ttttatctgt tagcgaatga ccagtacagg
     3601 cagattggcg tggcggatca cgctcgaggc gttagaacct aacagatggg tcgaaatcga
     3661 tgggttgcga gaaccgataa ctacaacatc agcccccagt tcttctgcca actcattgac
     3721 ttcatcccgc acgctaccaa aacggacatg ttgtttaatg cgggaaggat cgatggtgaa
     3781 gtggctgacc atcgtttgca gacgttcttc tgcttcatgt tgcagatgct cttcaaaacg
     3841 acgcacatca gcggcaaaac ggtgcaggct caggctggct gacccgggta gtacgtgaag
     3901 tagatgaata actccgtcat cctgggcgag gaattcagcg tggcgaacag ctttgtcgct
     3961 caattccatt tcaaatacat caactggcat aatgattgtc ttatacataa ccctttctcc
     4021 ctgttaatca tgaacaaatc attcgccatg attataatat ttatccctga tatttgtctg
     4081 gttctttttc cttacgaacg gtttctgtga ttaatatatt ctcactgaac accaggaatt
     4141 ctcccaaaac ctgcggtacc gcccgttttc ccgctgtgat agctaccctt aaagactgac
     4201 tcttttttga actgtctctg gaggttgcat gaaagcattg acttatcacg gcccacatca
     4261 cgttcaggta gaaaatgttc ccgatccggg cattgaacag gctgatgata ttattctgcg
     4321 tattacggca acggcgatct gtggctctga cctccatctt tatcgaggca aaatacccca
     4381 ggttaaacat ggcgatattt tgggtcatga atttatgggg gaagtcgttg aaaccggaaa
     4441 ggacgtaaaa aatttgcaaa aaggcgaccg ggtggtaatt ccgtttgtca ttgcttgtgg
     4501 cgactgtttt ttctgtcgaa tgcagcaata tgccgcctgc gaaaatacca atgcgggtaa
     4561 aggcgctgcg ctcaataaaa aacagatacc agctcccgcg gcattgtttg gttatagtca
     4621 cctgtatggt ggcgttcctg gtggacaggc ggaatatgtc cgcgtcccta aagggaatgt
     4681 ggggccgttt aaagtaccgc ctttgctttc agatgataaa gcccttttcc tttctgatat
     4741 tctgccaacg gcatggcagg cagcaaaaaa tgcgcagatc caacaaggtt caagcgttgc
     4801 agtctatggt gccggtccag tgggattgtt gacaatcgcc tgtgcacggt tgctcggtgc
     4861 ggaacagatt tttgttgttg atcatcatcc ctaccgcttg cgtttcgccg ctgaccgcta
     4921 cggcgcgatc ccgattaatt tcgatgaaga tagcgatccg gcacagtcaa ttattgaaca
     4981 aacggcaggt caccggggcg tggatgcagt aatagacgcc gtcggttttg aagcgaaagg
     5041 cagcaccacg gaaacggtgc tgactaacct gaaactggag ggctgcagcg gtaaagcgtt
     5101 gcgccagtgt attgcggcgg tcaggcgtgg cggcattgtt agcgtaccgg gcgtctacgc
     5161 tggatttatt cacggtttcc tgtttggcga cgcctttgat aaagggttga cgtttaaaat
     5221 gggacagacc cacgttcacg catggctggg tgaattatta ccgttaattg agaaaggatt
     5281 actgaaacca gaagaaattg ttacccacta tatgccgttt gaagaggccg cccggggata
     5341 tgagattttc gaaaaacgtg aagaggagtg ccgtaaggtg attctggtac ccggtgcaca
     5401 aagcgcagag gcggcgcaga aggcggtttc aggtctggtg aatgcgatgc cggggggaac
     5461 aatatgatcg tcaggagtgg ttttcgaggt aaaggacagc catgacgata atcgccgcca
     5521 taatcagaaa tcctatcagg atgtaaaatg cttctgccat ggttattccc acaaacgaaa
     5581 cggaataatt ttgcagcaaa agtgaacagt gagaaccagg aaaaattgct gattttgcgt
     5641 aaagaggatg cgagtgcatc ctctgggcaa agcgagttat cgcttgtgca gatgggatta
     5701 aagcaggtag tcgccagcag cttctggctg gtattcgagt tccagcactt caaggtgggt
     5761 tgcaacgccg cccggaagtt cccagtgaat ggaatcgcca acgcgcagcc ccagcagtgc
     5821 ggcacctacc ggagccataa cggaaagctg agtattgcta tcggtcattt ttgccggata
     5881 caccagcgtg cgcacacgca cttcgccatc gctaagattg cggaatttaa cccggctgtt
     5941 cattgtcacc acgtcgtgtg gcatctcttc tggcgaacac atttgggcgc gatccaactc
     6001 tgcgtttaac gcgtcggcga ttggcaaacc agcataggcg ggttgctcca gcagaatatc
     6061 gatgcgttcg gcatccaggt cgttaatgat gatagttggt ctggacattt ttactccatg
     6121 tcgtcggtac tgcgagtgtc gcagataaac atacccaaaa gaaaaccctc accgtcaggc
     6181 ggcgagggtt taactcacat gatgatactg actgttgctc actctttgaa gtgatttgcg
     6241 tcacattcag ggaattcctc aatgcagcgc attatgtata attcttaatc gtcacggttt
     6301 atggaagacg aattagcgtg ttttgtaaat cagatgatta ataacccact ttatcaatca
     6361 caaagttttt gccacagttg cctgggtgtg gttgtggcaa gaatgagttt gcagaaagcg
     6421 gagcgttgat ggcgtcagct ttgatcgaga tctgaatttc agtcaaatac gcagggttac
     6481 cctggcacgt tagcttaact gctttcacgt tctcttttcc ccagcttttg gcaaaggcgg
     6541 cgtcaaagtc acggcggctc actgttttac cgtaattatc cgcaaggaat tttccggctt
     6601 cgctctcttt aatttcttga ttcaggcgta ccatcgtacc gaagtatgcg tccggatcaa
     6661 aaccaaagca ggcaccgtgt ttggcatatt cgtagcgttc caggcaggaa cgtccgccag
     6721 ctcctggcat gacttcactt agtttagcgg ccgtttccag tgataacccg gtttccggcg
     6781 atgaacacat ccggctggcg tgcgcttctg gtagattcgg gattgggcga gtagcgcaac
     6841 cgaagcgcat ccagcggcgt tcatcaacac cacgggcagc aaccgattta ggcaatcctg
     6901 gccacagacc atgtacggtc agaaaatcag ctttgttggt cgtttcggtt tgtaggcgac
     6961 attcatctcg ttcgttacga tttcgatcgt gttgactctg gcaaaatccg gtttgccagg
     7021 agagggccag gacatagcga tcaaaatcgc catactgttt tgcctgcaac gctacggcgt
     7081 tggcagaaga gaagggaagc agagaaaccg cgagcaacgc ggcgttacgc cagaatgctt
     7141 tcataatggg tgtggaactc atacatacac tgaatactat ctattaaatc ataaaaaagc
     7201 ccgccaggtg ggcttacctg gcgggcgtga tgatttattc agcgtttggc gaacgtatta
     7261 gttccacatg gcgagaatcg gccagccaac caacagcaac atagagatgt aaatcacccc
     7321 gaagattgcg ccaagacgcc agtaatcttt tgatttcaca tagccacagc cgtaaataat
     7381 caccccagga ccggttgcat acggcgtcag acagcccatg ataccgatag acagcaccag
     7441 caggatacac agttgttcca ttggtacgcc cggaatacct ttaccgacgg ccagaataac
     7501 cgacagcatg gttgcggtgt gcgcagacag gctggcaaac aggtagtgtg caaagtagaa
     7561 caccagaacc agtacaatca ccgttgcgtt tggtgagaat ccttccaggt gcgtactcat
     7621 ggtaccggcg aaccagtcaa taaagccaga acgagtcagg ccgttagcca tcacaaccag
     7681 agttgccagg ttgaccagcg tgttccatgc gctgttatag cgggtaatat ctttccaagg
     7741 cacaacgtgc agggccagca ttagcgaaac tgccagcaga ccaactgcag tagcattaat
     7801 gacttcactg ccaaaaaccc acaaacctaa gctgagcaat acaaggccaa tcagtgtcca
     7861 ctctctgcgt gtcagcgcac ccatggtttt cagttcatca cccgcccagg ttgccacttc
     7921 ttcactgtgt gtgatttccg gtttgtacag cacgtaggaa agccacggcg caatgataag
     7981 caagataacc ccaaccggca ggaagcagag gaaccactgc aaccagctaa tctggatacc
     8041 ggcaattttg ctgacgaact ccagacccag cacgtttggt gccgcaccgg tgacaaacat
     8101 ggacgaactc agactggtac taatgaccat catccacatc aaatagccgc caatacgacg
     8161 cgcggacgga tcgttgggga atgatttaaa cagcggcggc aggtttttaa tgaccggaaa
     8221 aaccgtaccc ccggtacgcg cggtgttgga cggtgtaaac ggtgccagca gaatgtcgat
     8281 aatgacaatc gcataaccca acgtcagcgt gcgtttgccc atgaatttca ccaggaaaag
     8341 ggcaatgcga cgacctaacc cggaaacttc ataccctaat gcaaaaataa atgcgccaaa
     8401 taccagccat accgtggtgc tggaaaaacc agccagaccc catttcagcg cctgtttttg
     8461 cgcattaaac gctgggtcag ctaattcttt ggcatcaaag agcaggtaat tactgccaat
     8521 aacgcaaata gtaaccgcaa taaaactgat cgctgttgcc ggaattggct cgaggatcat
     8581 gccgacaatc attgccacaa acacagcgaa gtaatgccat gcctgtggtg gcataccgtc
     8641 ggggacaggg ataagaaaca tgacacccat caccaccagt ggggccaata gtttccatat
     8701 attatctttt gctaaagaca tacgggttct ccgaaaatta atatttccaa atttatcaag
     8761 tgcttaaata attaaatctg tgctaaaaac caggtaagga tcagcaggtc ggcactgccg
     8821 cctggactga gatttcgttc gatacactcc ctgtcgaact gccggagata atcgagatcg
     8881 gcgggggttc gaatgccccc tttttgcaat aatgtttgcg cctcgcgctg tagccagcgc
     8941 aggcccccct cgccaccgcg cgatgcaacg ttggtatcgc cgttgatcgc catcagtagg
     9001 agcaaggtat cgagcaatgc cagttcagga tctaacccct gatccagcag agtgaggtaa
     9061 tgcggcaagg cgtgattgat caccagtgga taacccgctt cggcttcacc gcgtgcgccg
     9121 gtaaggccaa gctgctggta caaccgttga cctgccgtca gttgtgaatt attggtacgc
     9181 agttctcgat cggtcaggcc acggcagaaa cttgccgccg tagaacaaac ggttgttggc
     9241 gttaccggtt ggttgagttg aagcaaacgg ccaattgccg cacatagtag ccctaaagaa
     9301 aaaatgctgc ctttatgcgt gtttacgccc gcagtggcgc ggaacatatc accttcgcaa
     9361 gccataccaa ttgggcgtaa tccgtggagt accgcttctg gtgctatttc cgcactacag
     9421 gcaccaaatt caatgaaacg gggtagccag ccctgaatcg ccagcgcgct gcggtggaaa
     9481 tcttccagcg ccatatcttt atgtgcaccg cagttaatcc gatccacgag gcctggtttc
     9541 ggtgacagat tgacttcagt cagcatggcg cgccagccca gcagggcgta ctcatcgatc
     9601 aatgacgtcg caagctttgt ggttttagtt gacgttgcag gcatcgacat cgttcagcag
     9661 tgcctccatg cggttgagta aatcggtcag ttgatgggtt tttccacgcg cgcagacggc
     9721 tgcgctttgc tcgcacaaca ggcagcggcg aggcggcagt gaatagtcgc ggcgggagag
     9781 aatttcgcct tcgggcgtca ggacatcgat atcccataac cgcccgagag gatgactatg
     9841 ttcaagctca atggtggcga gcttgaggtc gcgagccggg gcggcaatgc tcaacatgcc
     9901 ctccggcccg ctggcggaaa ccagtgcagc ctgctcctga atttgccagc cctgttttgc
     9961 ggctaaggca cgcaaggctg tcacgccatg attaaaaatt cggcgtgtga cctcgctgtc
    10021 tttaatcggc ccaggcgcaa ccacggtaaa ggagaccagt ggaacaggat ggcgcttgag
    10081 ccagacgtgt tgccgtgctt gcctttcatc ccggctgacg agcagctcgg gaattgacac
    10141 cgcatggtgg ctggcgagtt caggaagcag gtgcatggct tattccttca cctgatgcac
    10201 aacatcgatc accgagccat cgcggtaacg cacaacggca acgacgcggt cggtgaattc
    10261 aatcggctgt ggttcaccgg tcagcagacg cgcgcgttcg cgcagccact caatggaaac
    10321 cactttaatg cccgcttcct gcagacgttc tgccagttcc ggacgtgccg ggttaactgc
    10381 gataccgtgg tctgtgacca gaatatcgac actggagcct ggggtgatgc aggtcagcac
    10441 gttatccacc agagtcggaa tacgaccgcg taccagcggc gcgacgatga tggaaagcgc
    10501 agaggcaatc gcggtatcgc agtgaccacc ggaagcacca cgcagtacgc cgtcagagcc
    10561 ggtcagcacg ttaacgttga actgggtgtc aatttccagc gcgctcagta ccaccacgtc
    10621 gagacgatca accgatgcgc ctttcgaacc ccagttagcg tactggttgg cgctgatttc
    10681 gatgtgattg gggttacggg ccagcgattg cgcagcatgg ctgtcaaagc tctgcacatc
    10741 cagcagtttg cggatcagac ctttttcgtg cagatcaacc atcgtcgcgg taataccgcc
    10801 gagggcgaag tcggcgcgaa tatcgcggct acgcattttg tcttccagga aacgggttac
    10861 cgccagcgat gcgccgccgg tgccggtttg catggagaaa ccttctttga agtagccaga
    10921 gttgacaatc acatccgcag cgctacgggc aataagcagt tcgcgcgggt tagtggtcat
    10981 acgggtcgcg ccagcgccga tttttgcagc atcgccaacg cggtcaactt tgacgatcaa
    11041 atcaacctga tcttgctcaa tgcttgccgg attatgcgga taaggcagca gttcttcggt
    11101 aagcatcaca acctgttttg cgttgtcggc atcaaccatt gcatagccga gggagccgca
    11161 gcaggcttta ccggtgtaac cgttggcatt accgaattca tcacaggacg ggacgccgag
    11221 gaaagccacg tcgatattca gttcgccgct ctgtaccaga tgcacacgtc cgccgtgaga
    11281 gtggatttgc accggttccg caagcagacc acgggaaatt tcttctgcca gtggaccgcg
    11341 caggccggag gtataaatgc gggtaaccac gccctggcga atgtgttcta ccagtggcgc
    11401 atggcaatca ctcagggagc tggacgccag ggtcaggttt ttaaagccca tcttagcgat
    11461 gacgtccatc accatattga cggtcaggtc accgccacgg aaagcgtgat ggaaggaaac
    11521 cgtcatgccg tcctgtaaac cagagcgacg aatcgcttct tcctggttgg cgcacagttt
    11581 gcgatcgcgc gctttttcag cctggtaggt ttgctttggt gagttctgga aagcggcaag
    11641 atcgcattca gcgcgacgat tccaggccgc tacccgttct tgtcgttgag attgttcaat
    11701 tttctgcgtc attttgattg ccttattctt cgcggatgcc ggaaagttct gcacgggaga
    11761 gcaccagacg ggcacgatcg ataaccggac cgtccaccat cttgccgttc agggaaacca
    11821 cgccgaggcc ttcgcgagcg gcggcttcag cggcttctac gacgcggcgg gcgtgatcca
    11881 cttctttctg ggtcggtgcg tagaggttgt gcaacagatc aatctgacgc gggttgatca
    11941 gcgatttgcc gtcaaagccc agctgtttga tgtgggcggc ttcttgcagg aatccggctt
    12001 cgttgttagc gtcggaatag acggtatcga acgcctgaat acccgcagag cgcgcggcct
    12061 gcaaaatgga acagcgtgcg aacagcagtt cagttccttc cggggagcgt tctgtacgca
    12121 ggttgcgcac atagtcttct gcaccgaggg cgataccgat caaacgctcg gaagcgtgag
    12181 cgatttccac tgcgcgggta atccccagcg gagattcaat cgccgccagc aggccggtgc
    12241 tgccgggttc acgaccacag gctttttcga tacgcaggat ctctttttcg atatccagaa
    12301 catcctgagc ggtatcggtt ttcggcagac gcacaacgtc cgcaccaccg cgaacgacgg
    12361 cttccaggtc gttgacaccc cattcggaat ccagcgcgtt gacacgcaca atggtttcaa
    12421 tatcgcgata cagcggatgt tgcagcgcgt ggtaaaccat gcggcgggcg gtgtcttttt
    12481 cacgcaatgc tacggagtct tcgaggtcaa acatcagggc atcagccggg tagatgaagg
    12541 agttgctgac catcgcggca ttggcaccag gcacaaacaa catgctgcgg cgggtgcgag
    12601 ttttacgttg ttgcagcgaa gcggaaatca ttggcaatcc tcccatggca gagccgggat
    12661 accgctggcg cgtgccagca gggcttccag tcgtgcacgt aaaatgcagt ccagtgcgcc
    12721 tttgtcatcg acattcagct gtacgccgcg cacgttgtag cgggcgagaa cgtccagaat
    12781 ggtggtgcga attgcatcgc caaactgttt ctcaacgctg ctattgattt gcaggtcgat
    12841 atcctgcgta tcgagtgggg cgatgcgtat catcacatcc ccagactcaa gggtgcctgc
    12901 aacggcgggc tggtttattt tcatttttca cctgtttctc atgcgggggt cttttgacga
    12961 gctgccgcgt cctggcggga gtgctcaagc aggttctgca aataatgcag cgtgactgca
    13021 gggaccagcg gcgcgatagc cgtgagatcg tttttcgcca gcagttgacg tacccgggaa
    13081 gcggatatcg gcatctcctg gtaacgcagc cgctcaattt caaccagttc gatgggcggt
    13141 gcggagatag tcggcgtttc cagccagtag cgcatatcct ggttgtactg ggcggtaacg
    13201 cgacaaaagg gttcagtacc gacaaagcgg tgagttacac ccagcgcggg agcgaggtac
    13261 tgacggaaaa tcttcagatc aatttcggtg taacaatggt taatgacgct ctgttcttta
    13321 atgaagtagc aagggaacgt agcgcgggag atgatgtatt cggagccgcg atgcacagtc
    13381 aggcgtggaa tatcggcggt gccttttaac accagatcca accggtcttc ataggggaag
    13441 cgtgaagaat cttctttgac taaaaacaga tgcaaccagt cgcactgtgc cgcagcttgt
    13501 tgaatcagat aacggtgacc attcgtgaag ggattggcgt tcatcacaat gcagccaatc
    13561 ttgttccctg gatgacgaaa ttttttcagc gattcggcat agcgtttcag ccgcgtggcg
    13621 ctgttttcca tcagcaccat cacgccgggt acgctggtca gcgtggaaaa accgcactgg
    13681 cggaacagcg cctcgtattc ggttttggta taaataaaca gatgcgtgct gtgccgctca
    13741 taggcgaggt ttatcaattc agtggctaat gtcagcgcca gtccttcacc gcgaacggat
    13801 tcactgatag caacgcattt aataatattt ccggca
//
LOCUS       DAJTZL010000112        13793 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.112, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000112 DAJTZL010000000
VERSION     DAJTZL010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13793
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.112"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..146)
                     /locus_tag="OO544_002802"
     CDS             complement(<1..146)
                     /locus_tag="OO544_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HCR6768354.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKA"
     gene            complement(343..474)
                     /locus_tag="OO544_002803"
                     /pseudo
     CDS             complement(343..474)
                     /locus_tag="OO544_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310047.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
     gene            475..1020
                     /locus_tag="OO544_002804"
                     /pseudo
     CDS             475..1020
                     /locus_tag="OO544_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
     gene            complement(1062..2063)
                     /gene="gap"
                     /locus_tag="OO544_002805"
     CDS             complement(1062..2063)
                     /gene="gap"
                     /locus_tag="OO544_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCR6768355.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            2252..2782
                     /gene="cybB"
                     /locus_tag="OO544_002806"
     CDS             2252..2782
                     /gene="cybB"
                     /locus_tag="OO544_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HCR6768356.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVMYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            3027..3200
                     /locus_tag="OO544_002807"
     CDS             3027..3200
                     /locus_tag="OO544_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6768357.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            complement(3272..3421)
                     /gene="hokB"
                     /locus_tag="OO544_002808"
     CDS             complement(3272..3421)
                     /gene="hokB"
                     /locus_tag="OO544_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HCR6768358.1"
                     /translation="MKHNPLVVCLLIICITILTFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            3820..5460
                     /gene="trg"
                     /locus_tag="OO544_002809"
     CDS             3820..5460
                     /gene="trg"
                     /locus_tag="OO544_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HCR6768359.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASCIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMKDEPAGRNEIGRLNRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVDVFRLHKHSVSAEPRGAGEPVSFATV"
     gene            complement(5498..6421)
                     /locus_tag="OO544_002810"
     CDS             complement(5498..6421)
                     /locus_tag="OO544_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6768360.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            6638..7981
                     /locus_tag="OO544_002811"
     CDS             6638..7981
                     /locus_tag="OO544_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR6768361.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQTRSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            8206..9861
                     /gene="opgD"
                     /locus_tag="OO544_002812"
                     /gene_synonym="mdoD"
     CDS             8206..9861
                     /gene="opgD"
                     /locus_tag="OO544_002812"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HCR6768362.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYALKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            10001..10225
                     /locus_tag="OO544_002813"
     CDS             10001..10225
                     /locus_tag="OO544_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HCR6768363.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            10288..10824
                     /gene="rimL"
                     /locus_tag="OO544_002814"
     CDS             10288..10824
                     /gene="rimL"
                     /locus_tag="OO544_002814"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HCR6768364.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(10819..11799)
                     /locus_tag="OO544_002815"
     CDS             complement(10819..11799)
                     /locus_tag="OO544_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LbetaH domain-containing protein"
                     /protein_id="HCR6768365.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKSVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIRDSLVYGQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ISGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            11923..12915
                     /gene="tehA"
                     /locus_tag="OO544_002816"
     CDS             11923..12915
                     /gene="tehA"
                     /locus_tag="OO544_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HCR6768366.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSENGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            12912..13505
                     /gene="tehB"
                     /locus_tag="OO544_002817"
     CDS             12912..13505
                     /gene="tehB"
                     /locus_tag="OO544_002817"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HCR6768367.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDRQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
BASE COUNT         3540 a         3196 c         3482 g         3575 t
ORIGIN      
        1 gccttacggg catcctcggc ctgaccatcg ggtatacggg aaatgacagc ctctgtagca
       61 gggtttacca catcaatcca tgcgtctcca cgccaggtaa caaactgtcc atcgatatac
      121 ataggatgtt gaacgggtac tgacatgggc gactcctgtg atttatatgt ttgttttcgg
      181 tgaatatatt gttaattaac atcaatcatc agagagacgg catcgggaaa ggacaattct
      241 gtgaagggct tcataaaaac aagtgtaaaa ggaataaaag gcagaaataa aagtcaggag
      301 tcatgcccgt ttgcaaacaa gtggttcaca aacgggcaat ttttaacgta acgagcgact
      361 ttccatcgcc tcgatgagca ccgcatcatg tttcagcgtt tgccatgcat ggatgacttc
      421 tgccggaaaa tcaacgtgaa cgataaaatc tcgcccgcgg ggaccgtagc cattgatcag
      481 ttcgtcaatc ggctgggtga agcgatgcag gcggttaaat ttggtaaccc cgctgaacgc
      541 aacgacattg cgatggggcc gttgattaac gccgcggcgc tggaaagggt tgagcaaaaa
      601 gtggcgcgcg cagtagaaga aggggcgaga gtggcgttgg gtggcaaagc ggtagagggg
      661 aaaggatatt attatccgcc gacattgctg ctggatgttc gccaggatat gtcgattatg
      721 catgaggaaa cctttggccc ggttctgccg gtggtcgcat ttgacacgct ggaagaggct
      781 atctcaatgg ctaatgacag tgattacggc ctgacctcat caatctatac ccaaaatctg
      841 aacgtcgcga tgaaagccat taaagggctg aagtttggtg aaacttacat caaccgtgaa
      901 aacttcgaag ctatgcaagg cttccacgcc ggatggcgta aatccggtat tggcggcgca
      961 gatggtaaac atggcctgca tgaatatctg cagacccagg tggtttattt acagtcgtaa
     1021 tgagtaaaag aggcggaggt tttttcctcc gcctgtgcgc gtcagagttt agcgaatttt
     1081 tcgagggtgc gaataagctg cgtgacgaag ccatattcgt tatcgtacca ggcgaccgtt
     1141 ttcaccagtt gtaaatcgcc cacggcggta atttccgttt gcgtggcatc aaacaccgaa
     1201 ccgaaatggc tgccaatgat atcggaagag actatttctt catcggtata accaaatgac
     1261 tcgttattgg tggttgcttg tttaagtgcg ttattcacct cttcggcagt cacttttttt
     1321 ccgagaatcg ataccagttc agtgaccgaa cctgttttca ccggcacgcg ttgcgcatga
     1381 cctttcagtt tgccgctcag ttccgggatc accagaccaa tggcttttgc cgcccccgta
     1441 gtgtggggaa tgatattttc tgccgctgcg cgtgaagcac gtaaatcttt accacgtggg
     1501 ccatccacca gtgactgggt gccagtatag gcatgaatgg tcgtcatcgt gccgacttct
     1561 atcccgaaac tgtcatgcaa ggctttggcc atcggcgcaa gacagttagt ggtgcatgac
     1621 gccacggaaa caatggtgtc gttgccatcc agagtgtcgt cattgacgtt ataaacgata
     1681 gttttcattt caccggcagg ggcggaaatc aacaccttct tcgcaccagc atcaagatgt
     1741 gcctgcgatt tctcggcgga ggtataaaag ccagtacatt cgacaatgat ttctgcacct
     1801 ttcgctttcc acggaatatt tttagcctct ttttcggcgt aaaccgcgat acttttccca
     1861 tcaacgataa gtgaatcttc cgtaaaatca acgctccagg ggaatggtcc gtagtttgaa
     1921 tcatgtttca gcaggtaggc gagaattttt ggggaagtga gatcattaat agcgacaacg
     1981 tctatgttgc ttttgacttc aagtaatcga cgcaacacca gtcgaccgat acgaccaaaa
     2041 ccgttaatac caactttact catggttttc tcctgtcagg aacattcgga tgaaaattga
     2101 tcctttccag gcttagacca ggattgtggg atgggcaatc tccattctta cagtgaaacg
     2161 taacgtactg aaaacgggtg aacaatattt aatgaaattt tgagaaaagc ccgttatgtt
     2221 aacggaaaat tatgttaaag caggaaatgt tatggaaaat aaatactcaa ggttacaaat
     2281 cagcattcac tggctggtct ttttgctggt tatcgcagcg tattgcgcaa tggagtttcg
     2341 tggtttcttc ccacgtagcg atcggccact catcaacatg attcatgttt cctgcggcat
     2401 ctcaattctc gtgctgatgg tcgttcgtct tttgttaagg ctgaaatacc caaccccgcc
     2461 gattatacct aagccaaaac cgatgatgac gggactggcg catttgggac atttggtgat
     2521 gtatctcctg tttattgcgc tgccagtgat tggtttggtg atgatgtata accggggcaa
     2581 cccgtggttt gcgtttggtt tgacgatgcc ttacgcttca gaggccaatt tcgaacgggt
     2641 tgatagctta aagtcgtggc atgagacgct ggcgaatctg ggatattttg tcatcgggtt
     2701 gcacgctgcg gctgcactgg cacaccacta tttctggaag gacaacacac ttctacgcat
     2761 gatgccgcgt aaacgttcct gaaggatatt tcaagaaaat gtctgtacta aaaccgaccc
     2821 gtggtacagg cgaagaatac gggtctacat cggaagcgcc tattatattt atttgtatga
     2881 caaataaaac cataatcctt gcccatacgt ccatctggct tatttttaat caattcaccc
     2941 gatctttgat ctcatcaacg gtatcaaaat aaaccagcgt ataacgttca aatcgaacat
     3001 aagattcgat aaaccatgga ggttatatga aaaaactggc acttattttg tttatgggaa
     3061 cgcttgtttc cttttatgcc gatgccgggc gcaaaccctg ttctggttcg aaagggggga
     3121 tctcacactg tacggcaggc ggcaaatttg tctgtaatga tggttctatt agtgcatcga
     3181 aaaaaacatg cactaactga agtgtaaaag gggtgccatg agaaaatgaa tctgctagtc
     3241 agatgcgcgg ggaaaatccc cgcgcttgcc cttacctgga cgtgcaggcc atgagcgcag
     3301 caacctcctt atcaccgtcc cggaaccgca gttcgtagag cgtttgtcgg gtcaggagtg
     3361 tgaatgtcag aatcgtaatg cagataatga gcagacacac taccagaggg ttgtgcttca
     3421 tagcctttct ccttgccgga tggcgggtaa gaggctaaga tctgaattgc taggttcatt
     3481 cgttggcctc ggttgataga aatatcggtc ggggccttcg tctttctgat tcccggttag
     3541 cctgaaaaca gaaagtctca ggcacccgca ggcatcctat gaggtttcct tagggacgaa
     3601 aataatcact tcacgaaatt gcgtgctgtt ttccagaatt tttcgtcatt cgggttagcc
     3661 agtttagcca ttcgttactc tcttcattcc aatagcatta attttctctg caataattgt
     3721 tgtaaaaatg tgacgcaaag aggtttttgg tcataagtaa ttaccgtcaa gtgccgatga
     3781 ctttctatca ggagtaaacc tggacgagag acaacggtaa tgaatacaac tccctcacag
     3841 cgattaggtt ttttgcatca catcaggctg gttccgttat ttgcctgcat tcttggcggt
     3901 atcttagttc tattcgcatt aagttctgct ctggctggct atttcctctg gcaggccgat
     3961 cgcgatcagc gtgatgttac tgcggagatt gagattcgga ccgggttagc gaacagttca
     4021 gattttttgc gttcagcccg gatcaatatg attcaggccg gggctgcgag ttgtattgcg
     4081 gaaatggaag caatgaagcg aaatattgcg caagccgaat cggagattaa acagtcgcag
     4141 caagggtatc gtgcttatca gaatcggccg gtgaaaacac ctgctgatga agccctcgac
     4201 actgaattaa atcaacgctt tcaggcttat atcacgggta tgcaacctat gttgaaatat
     4261 gccaaaaatg gcatgtttga agcgattatc aatcatgaaa gtgagcagat ccgaccgctg
     4321 gataatgctt ataccgatat tttgaacaaa gccgttaaga tacgtagcac cagagccaac
     4381 caactggcgg aactggccca tcagcgcacc cgcttgggtg ggatgttcat gattggcgcg
     4441 tttgtgcttg ccctggtcat gacgctgata acatttatgg tgctacgtcg gatcgtcatt
     4501 cgtccactgc aacatgccgc acaacggatt gaaaaaatcg ctagtggcga tctgacgatg
     4561 aaggatgaac cggcgggtcg taatgaaatc ggtcgcttaa atcgtcattt acagcaaatg
     4621 cagcattcac tggggatgac agtagggact gttcgacagg gtgcggaaga gatttatcgt
     4681 ggcaccagcg aaatttcagc tggcaatgcg gacctgtcat ctcgcaccga agaacaagcg
     4741 gcggctatcg aacaaactgc cgccagcatg gagcaactca ctgcgacggt gaaacagaat
     4801 gcggataacg cgcatcatgc cagcaaactg gcgcaagagg cttctattaa agccagcgat
     4861 ggcgggcaga cggtttccgg tgtagtaaaa acgatgggcg ctatctccac gagttcgaag
     4921 aaaatttctg agatcaccgc cgtcatcaac agtattgctt tccagacgaa tattctggca
     4981 ctgaatgctg ccgttgaagc cgcgcgagcg ggtgagcaag ggcgtggatt tgccgttgtc
     5041 gccagcgaag tacggacact cgcaagtcgc agcgctcagg cggcgaaaga gattgaaggc
     5101 ttgatcagtg aatcagtcag gttaattgac ctggggtcgg atgaggtggc aacggccggg
     5161 aaaaccatga gcactattgt tgatgccgtc gcgagtgtca cacatatcat gcaggaaatc
     5221 gccgccgcct cggatgaaca aagtagaggc ataacgcagg ttagccaggc gatttctgaa
     5281 atggataagg tgacgcaaca gaatgcttct ctggtagaag aggcctcagc ggcggcggtg
     5341 tcccttgaag aacaggcggc acgattaact gaggcggtgg atgtattccg tctgcacaaa
     5401 cattctgtgt cggcagaacc tcgcggagcg ggtgaaccag ttagtttcgc tacggtgtga
     5461 aaatgttcaa ggagggatcg acagatccct tcacctttca gaacggcatt gattttcgaa
     5521 tagcgttaat catcaactgg caaccagaag agaacgtcgc atctacgcgg gtcagtattc
     5581 caatcggttc gcctgcacca tgtcccggaa caggcagggc caccagcgtg gcatgacgca
     5641 ggtcgtcttt tacagcgcca gaagggacaa accacacgta atcgtattca accgtaagtt
     5701 gacgagatag cgaagcagac agcgtttcga tacaacccga aggaatttta cagccctggc
     5761 tctgcactaa tgcatctgaa tgctggcgtg gcgcagtgcc ttctggtgat acaacgaccg
     5821 gccattccag cacccggctt agcgttacgt tctcctgaag tagcgggtga ttagggcgga
     5881 caaccagctt cagcgattca agaaacagca gttcgtaatt aagcccggtc atcagttcag
     5941 gatctgacat ccgaccaatg ccgatatcga tttccccggt ttttaaaccc gccagaatca
     6001 tagggttact cattgtcgca acttgcaagg tcgtctcttt ttgttgctga tgaaactgac
     6061 ctataaccga aggtaatatc cccagtgccg cagtaggtag tgcaccaacc ctgacgacat
     6121 cattattaag accttcttta cgatgaagcg actgtccggc agtgttgatg gcgtcaagaa
     6181 ctctgactgc atgcgttaaa aattgttcgc cgggtaaggt aagttgcgcc ccctgacgac
     6241 cacgctcaaa caagcgagcg cccgtcagct gctccagttc attcaatgtc ttagagagcg
     6301 caggttgact caaattaagg gtttcagccg cgcgccccaa agttccttgt tgtgcgacag
     6361 ctacgaatgt atgaaggtgg cgcaaacgga tgcgctgact aaacagacta tttttttcca
     6421 taagcgatgt taaaaacgaa gcggtgtcgc tgacaagtga agttgtttga ttatgataac
     6481 ttgattgcaa aatattatta acaattaaag caattatgtt acagcaaaat ggataatatt
     6541 gatgttttcg cggcgagatc acagtttgta aattcttccc gccagagtga atgcggttac
     6601 ctacactcca gattactgac cactggaggc agacactatg gcgaacagca tcacggcgga
     6661 tgagattcgg gaacagtttt cgcaggcaat gtcagccatg taccagcaag aagttccgca
     6721 atatggcacg ctgctggaac tggtagctga tgtgaatctg gctgtgctgg aaaacaatcc
     6781 tcaactgcac gaaaaaatgg taaatgcaga cgagctggcg cgactgaatg ttgaacgtca
     6841 tggggcgatt cgcgttggga ctgcacaaga gcttgctact cttcggcgga tgtttgccat
     6901 tatggggatg tacccggtga gctattacga tctctcgcag gcaggggtgc cggtacattc
     6961 gacagcattt cggcccattg atgatgcttc tctggcgcgt aatcccttcc gcgtttttac
     7021 ctccttactc cgccttgagc ttatcgagaa cgaaattttg cgccagaaag cggcggagat
     7081 tctgcgtcag cgcgatatct tcaccccacg ttgtcgacaa ctgttagagg aatatgagca
     7141 gcagggcggt tttaacgaaa cacaggcaca ggagtttgtg caggaagccc tggaaacgtt
     7201 tcgctggcac cagtcagcaa cggtagatga agaaacctat cgcgccttgc acaacgaaca
     7261 tcggttgatt gctgatgtgg tctgttttcc tggatgccat atcaaccacc tgacgccacg
     7321 tacgctggat attgaccggg tgcagtcgat gatgcctgaa tgcggaattg aacccaaaat
     7381 tctcatcgag gggccgccgc gccgcgaggt accgatttta ctacgccaga ccagctttaa
     7441 agcactggaa gagacggtgt tgtttgcggg gcagaaacag ggcacgcata ccgcgcgctt
     7501 tggtgaaatt gagcagcgtg gcgtggcatt aacgccgaaa gggcgacaac tgtatgatga
     7561 tcttctgcgt aacgctggaa ccgggcagga taatctcact caccaaatgc atttacagga
     7621 aaccttccgc acttttcctg acagtgagtt tttaatgcgt cagcaagggt tggcatggtt
     7681 ccggtaccgt ctgacgcctt cgggtgaggc gcatcgtcag gcgattcatc ctggagacga
     7741 tccacagccc ttaattgaac gtggttgggt agtggcgcaa cccatcacct atgaagattt
     7801 cttgcccgtt agcgcggcgg ggatcttcca gtcaaatctg ggtaatgaaa cgcagacacg
     7861 cagtcacggt aatgccagtc gcgaagcatt tgagcaggcg ttgggttgtc cggttttgga
     7921 tgagttccag ctttaccagg aagcggaaga acgcagtaaa cgtcgctgtg gtttgcttta
     7981 aattctgacc atccgccttt gcaaaaattt gcctgatttt acaaacgaat caggctcatc
     8041 ccatcgacat aaaaaaaatg ccgatttatg catattctct cagttcaaca attggattat
     8101 taataaatat tgtctagagt gagcggtcat aaataagcac tttcttgccg ctgaaaacga
     8161 ccagcgcggg accattcaca acaccagaag gactcacttt caggtatgga tcgtagacga
     8221 tttattaaag gttcaatggc tatggccgcc gtgtgcggta ccagcggcat tgcttctctt
     8281 ttttctcagg cggcattcgc ggcagattct gatattgccg acgggcaaac ccagcgtttt
     8341 gacttctcca ttctacagtc aatggcgcac gacttagcgc aaacagcgtg gcgtggtgcg
     8401 cctcgtccgt tacctgacac gctggcgaca atgacgccgc aggcttataa cagtattcaa
     8461 tacgacgccg aaaaatcgct ctggcataac gttgagaacc gtcaactgga cgctcagttc
     8521 ttccatatgg gaatgggatt ccgtcgccgc gttcgtatgt tttctgtaga tccagcaaca
     8581 catctggcgc gtgaaattca ctttcgcccg gagttgttca aatacaacga tgcaggtgtt
     8641 gataccaaac aattagaagg gcaaagcgat ctcggctttg ccggttttcg cgtgtttaaa
     8701 gcccccgaac tggcgcgccg tgatgtagta tcatttctcg gcgcgagtta tttccgcgcc
     8761 gttgatgata catatcaata cggtttgtcg gcccgcggcc tggcgatcga cacttacacc
     8821 gacagtaaag aagagttccc cgactttacc gccttctggt ttgatacggt aaaaccgggg
     8881 gcaactacct ttaccgttta tgcgttgctc gatagcgcca gtattactgg tgcctataag
     8941 ttcactatcc attgtgagaa aagtcaggtg attatggatg tggaaaatca cctgtatgcg
     9001 ctcaaagaca ttaaacagct gggcattgcg ccgatgacca gtatgttcag ctgcggtact
     9061 aatgaacgtc ggatgtgcga caccattcat ccgcaaattc atgactctga tcgtctgtcc
     9121 atgtggcggg gcaacggcga gtggatttgc cgtccgctga acaatccgca aaaattgcag
     9181 ttcaatgctt acaccgacaa caacccgaaa gggtttggtt tattgcaact ggatcgtgac
     9241 ttctcccatt atcaggacat tatgggctgg tataacaaac gcccaagttt gtgggtggaa
     9301 ccgcgtaaca agtggggtaa gggcaccatc ggcctgatgg aaatcccaac aacgggcgaa
     9361 acgctggata acattgtctg cttctggcag ccagaaaaag ctgtaaaagc aggtgatgag
     9421 tttgcattcc agtatcgtct gtactggagt gcgcaaccgc ctgttcattg cccattagcg
     9481 cgcgttatgg cgacgcgtac cggcatgggc ggtttcccgg aaggttgggc gccaggtgaa
     9541 cactatcccg aaaaatgggc gcgtcgtttt gccgtcgatt tcgttggtgg tgatctgaaa
     9601 gctgccgcgc caaaaggcat tgagccggtg attacgcttt ccagtgggga agcgaagcaa
     9661 atcgaaattc tctatattga acccatcgat ggttatcgta ttcagtttga ctggtatccg
     9721 acttcggact ctactgatcc ggtcgatatg cggatgtatc tacgttgtca gggggacgct
     9781 atcagtgaaa catggctgta tcagtatttc ccgccagcgc cggataaacg tcagtatgtt
     9841 gacgaccgcg tgatgagtta atcgtttttt cttcggcacc ttcttcggga ggtgccgtct
     9901 ggttaaacac gatcccgctc gcatttttcc ctaagttaaa tgagtaatct gatggtgtgt
     9961 atttcagata caccttgtca gccactaaca gggagtgcgt atgtttccag aataccgaga
    10021 tttaatatcc cgtctgaaaa acgaaaatcc tcgctttatg tccttgttcg ataaacacaa
    10081 taaacttgat catgaaattg ccagaaagga aggttccgac ggtcgagggt acaatgcgga
    10141 agtggtccgc atgaaaaaac aaaagctaca gttaaaagat gagatgctca aaatcctgca
    10201 gcaggagagc gtcaaagagg tgtaaacttt cctaagccgc cagccaggcg gcttttttaa
    10261 caacagcatg gattgactgg agataagatg actgaaacga taaaagtaag cgaatcactt
    10321 gaattacatg ctgttgcgga aagtcacgtc acacctcttt atcagttaat ctgtaaaaat
    10381 aaaacctggt tacagcagtc gctaaactgg ccgcagttcg ttcagagtga agaggacacg
    10441 cgaaaaacgg tgcagggtaa tgtgatgttg catcaacgcg gctatgccaa aatgttcatg
    10501 attttcaaag aagatgaact tatcggcgtt atctcgttta atcgtattga accactgaat
    10561 aaaactgctg aaatcggcta ctggctggac gaatctcatc aggggcaggg gatcatttct
    10621 caggcgctgc aggcattgat tcatcattac gctcagtctg gtgaacttag acgcttcgtg
    10681 atcaaatgtc gggtggacaa tccgcaaagc aaccaggtcg ctttgcgcaa tggttttatc
    10741 cttgaaggtt gcctgaaaca ggctgagttc ctgaatgatg cctatgatga tgtgaactta
    10801 tacgcgcgta ttatcgattc ataataaccc tgccagcggc gttcgcgtaa tgcgctcctc
    10861 gccgttaatg acttttggac cacggacatg aaccgtgtcg ccatcaaagg cttcaacgac
    10921 ggcgtggtcg gttaactcca catggttttc aattatcacc gcacctgaaa tacggctctc
    10981 accttgtatt acaacgtgct cgtcgagcag aatcggtcca ccacgtacca cggcatttcc
    11041 gccaatcaag acatgatgtt ttaacacaca attaccttcc acaatggcat attccgccac
    11101 ctgcgaactg taatgaatcg tcggaatggc atcttcttct atgccagctt tcacctgcgc
    11161 gtgaccgtag acttttgcgc aatcgcatag ccagacgttg ttctcttcat taccttcaac
    11221 actggcaaaa tcaaaaactt cggcgcgatg ttcaataaaa gcataccgga cgacggcatc
    11281 gccataaatt tgtgcctggt ggacaatacg cgaggcgcta acccttgcgc gatcataaat
    11341 ttggagcagg agttgatggt cgggcgttaa gccttgtgcg gcgacgatca tagaatgttg
    11401 atcaattaag gcgtgaccaa atattctaca ttgcccgtaa actaatgagt cacgaatggt
    11461 gacactgtca cttatatatg caccctggct gatttcacta ttatcgatcc agacgttatc
    11521 cgttgcgtaa acctctcccc acagcacgct ggtgccggtg atacgcgtat taccagaaat
    11581 caccgttccg ccgaacgcaa tagcattctg gtcgtaaatc cagcaatttc cttcctgtgc
    11641 taacaccgtt tcgcgatcga tccagccgcc agcggttcct gctatcacat cgttaaaatc
    11701 gctaatggcg ataatctgcc gtaataacac acttttttta gtgccatctt cctgataact
    11761 aaaggctcgc tgttcttcac ttagacgata tttgcgcata gcgttttctc acaggtgact
    11821 tactataacc gtagcaaatt ctgcggctct ggctatgctc gagaaaattc cataaaatgc
    11881 atttcaaata tactttataa attaaacaaa atgagtaaga agatgcagag cgataaagtg
    11941 ctcaatttgc cggcaggcta ctttggtatt gtgttgggga cgatagggat gggatttgcc
    12001 tggcgctatg ccagccaggt ttggcaggtc agccactggt taggggatgg gctggtgatt
    12061 ctggcgatga tcatctgggg attattaact agcgcattta ttacccgact catacgcttt
    12121 ccgcatagcg tgctggcgga agttcgccat ccagtgctga gcagttttgt gagtttgttt
    12181 ccggcaacga cgatgctggt ggcgattggt tttgttccgt ggtttcgccc actggcggtg
    12241 tgcctgttca gttttggtgt cgtggttcag ttggcttatg ccgcctggca aactgcggga
    12301 ttatggcgcg gatctcaccc tgaagaagct accacgcctg gactgtatct gccgacagtt
    12361 gccaacaact ttatcagcgc aatggcctgt ggtgcgttgg gctacaccga cgccggtctg
    12421 gtgtttttag gcgcaggcgt tttctcatgg ctaagtcttg aaccggtgat cttgcagcgt
    12481 ctgcgcagtt cgggagaatt acccacggca ctgcggacat cactcggcat tcagctcgct
    12541 cctgcgctgg tggcctgtag tgcctggttg agcgtcaacg gcggcgaggg tgatacgctg
    12601 gcgaaaatgc tttttggtta tggactgctg caactgctgt ttatgctacg tctgatgcca
    12661 tggtatctct cccagccatt taatgcttca ttctggagtt tctcgttcgg cgtatctgca
    12721 ctggcaacca ccggtttgca tctggggagt ggcagcgaaa atggattttt ccatacgctg
    12781 gcggtaccgc tgtttatctt taccaatttt attattgcaa tactgctcat ccgtactttt
    12841 gcgcttctga tgcagggaaa attgttagtc agaaccgagc gcgccgtttt aatgaaagca
    12901 gaggacaaag aatgatcatt cgtgacgaaa actattttac tgataaatat gaattaaccc
    12961 gcacacactc tgaagtactg gaagcggtga aagtggttaa accgggtaaa acgctggatc
    13021 tgggctgtgg caatggtcgt aacagtcttt acctggcagc caatggttat gatgttgacg
    13081 catgggataa aaatgccatg agtatcgcca atgtagagcg cattaaatcc attgaaaatc
    13141 tggataattt acacacccga gtcgttgatc tgaataacct cacatttgat agacagtacg
    13201 attttattct ttcgactgtg gtactgatgt tccttgaggc taaaaccatc ccagggctga
    13261 ttgccaatat gcaacgttgc actaaacctg gtggttacaa cctgattgtg gcggcgatgg
    13321 ataccgctga ttatccatgt accgtcggct tcccgtttgc cttcaaagag ggagaattac
    13381 gtcgatatta cgaaggctgg gagagggtga aatacaatga agacgtcggc gagctgcacc
    13441 gcaccgacgc caacggtaat cgtattaaac tgcgtttcgc cacgatgctg gcacgtaaaa
    13501 aatgacccgc taagaataag gcgcgtgaaa attccccacg ctgagatgat ttactgttct
    13561 tcttttcggt aagcatattt tttatcgaag ggatgtgaaa ttaatcacag tagtcgaagt
    13621 tttttagcag cttaacttac tgaaatttaa ctactgatga ttgacttagc ccctttttcg
    13681 gcattgacta tgtcgtctga aaaggggctg aaaaatttat tttcaccaac actttttttg
    13741 ccacaacacg aagcggcgct ttttgctata acttaaaaag taatataatc atc
//
LOCUS       DAJTZL010000113        13749 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.113, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000113 DAJTZL010000000
VERSION     DAJTZL010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13749
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.113"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            254..2020
                     /gene="adeD"
                     /locus_tag="OO544_002818"
     CDS             254..2020
                     /gene="adeD"
                     /locus_tag="OO544_002818"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HCR6768368.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     GGEISGPIVIKGRYIAGVGAEYADAPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGL
                     GGKELNAYITAGIENCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLAQSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSHSSVYSENGFDRDDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDVEKFAFTTLEVTE"
     gene            complement(2065..3456)
                     /gene="uhpT"
                     /locus_tag="OO544_002819"
     CDS             complement(2065..3456)
                     /gene="uhpT"
                     /locus_tag="OO544_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HCR6768369.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(3594..4913)
                     /gene="uhpC"
                     /locus_tag="OO544_002820"
     CDS             complement(3594..4913)
                     /gene="uhpC"
                     /locus_tag="OO544_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HCR6768370.1"
                     /translation="MLPFLKAPADAPLMADKYEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIINILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAAAALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            complement(4923..6425)
                     /gene="uhpB"
                     /locus_tag="OO544_002821"
     CDS             complement(4923..6425)
                     /gene="uhpB"
                     /locus_tag="OO544_002821"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HCR6768371.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLVLP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(6425..7015)
                     /gene="uhpA"
                     /locus_tag="OO544_002822"
     CDS             complement(6425..7015)
                     /gene="uhpA"
                     /locus_tag="OO544_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HCR6768372.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(7090..7380)
                     /gene="ilvN"
                     /locus_tag="OO544_002823"
     CDS             complement(7090..7380)
                     /gene="ilvN"
                     /locus_tag="OO544_002823"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCR6768373.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(7384..9072)
                     /gene="ilvB"
                     /locus_tag="OO544_002824"
     CDS             complement(7384..9072)
                     /gene="ilvB"
                     /locus_tag="OO544_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HCR6768374.1"
                     /translation="MASSGTTLTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIEAQPAVAEKAAAPAFSEESIRDVATMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPQVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPSPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(9178..9276)
                     /gene="ivbL"
                     /locus_tag="OO544_002825"
     CDS             complement(9178..9276)
                     /gene="ivbL"
                     /locus_tag="OO544_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HCR6768375.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            9841..9930
                     /gene="tisB"
                     /locus_tag="OO544_002826"
     CDS             9841..9930
                     /gene="tisB"
                     /locus_tag="OO544_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HCR6768376.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            10210..11394
                     /gene="emrD"
                     /locus_tag="OO544_002827"
     CDS             10210..11394
                     /gene="emrD"
                     /locus_tag="OO544_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HCR6768377.1"
                     /translation="MKRHRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSSVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(11402..11899)
                     /locus_tag="OO544_002828"
     CDS             complement(11402..11899)
                     /locus_tag="OO544_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HCR6768378.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRCDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(11896..12258)
                     /locus_tag="OO544_002829"
     CDS             complement(11896..12258)
                     /locus_tag="OO544_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HCR6768379.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(12248..12595)
                     /locus_tag="OO544_002830"
     CDS             complement(12248..12595)
                     /locus_tag="OO544_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HCR6768380.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            12704..13153
                     /locus_tag="OO544_002831"
     CDS             12704..13153
                     /locus_tag="OO544_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6768381.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDVDVDKILDQSGIALAIAALLCAFVGGMRKE
                     NRWGIRGALVFGGGTLAFHTLLFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(13402..>13749)
                     /locus_tag="OO544_002832"
     CDS             complement(13402..>13749)
                     /locus_tag="OO544_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6768382.1"
                     /translation="NVRAKSGLNRSILDQGWYEMRRQLAYKQLWRGGQVLAVPPAYTS
                     QRCVCCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRS
                     LKQEPTEMIQATA"
BASE COUNT         3267 a         3811 c         3564 g         3107 t
ORIGIN      
        1 tcccatgctg aggtagttta aataatcgcg aaagcgtccc tgattcatta ctcacgtaat
       61 cggtattgtc attattcatt tttttgtcca ttggaggaga tttaatccct tgcttttgta
      121 aaacaaatga caacaacggt tcagtgataa ttatcacatt tcaattgcac attaatggat
      181 attctttaat aatctcgcga cgtttcttta tgataaataa taatcaaatt gataaaatca
      241 aaatgagaaa aatatgaata attctattaa ccataaattt catcacatta gccgggctga
      301 ataccaggaa ttgttagccg tttcccgtgg cgacgctgtt gccgattata ttattgataa
      361 tgtctctatt ctcgacctga tcaatggcgg agaaatttcc ggcccaattg tgattaaagg
      421 acgttacatt gccggtgttg gcgcagaata cgctgatgct ccggctttgc agcggattga
      481 tgctcgcggc gcaacggcgg tgccagggtt tattgatgct cacctgcata ttgaatccag
      541 catgatgacg ccggtcactt ttgaaaccgc taccctgccg cgcggcctga cgaccgttat
      601 ttgcgaccct catgaaatcg tcaacgtgat gggagaagcc ggattcgcct ggtttgcccg
      661 ctgtgccgaa caggcaaggc aaaaccagta cttacaggtc agctcttgcg tacccgccct
      721 ggaaggctgc gatgttaacg gtgccagttt tacccttgaa cagatgctcg cctggcggga
      781 ccatccgcag gttaccggcc ttgcagaaat gatggactac cctggcgtaa ttagcgggca
      841 gaatgcgctg ctcgataaac tggatgcatt tcgccacctg acgctggacg gtcactgccc
      901 gggtttgggt ggtaaagaac ttaacgccta tattactgcg ggtattgaaa actgccacga
      961 aagttatcag ctggaagaag gacgccggaa attacaactc ggcatgtcgt tgatgatccg
     1021 cgaagggtcc gctgcccgca atctcaacgc gctggcaccg ttgatcaacg aatttaacag
     1081 cccgcaatgc atgctctgta ccgatgaccg taacccgtgg gagatcgccc atgaaggaca
     1141 catcgatgcc ttaattcgcc gcctgatcga acaacacaat gtgccgctgc atgtggcata
     1201 tcgcgtcgcc agctggtcga cggcgcgcca ctttggtctg aatcacctcg gcttactggc
     1261 acccggcaag caggccgata tcgtcctgtt gagcgatgcg cgtaaggtca cggtgcagca
     1321 ggtactggtg aaaggcgagc cgattgatgc gcaaacctta caggcggaag agtcggcgag
     1381 actggcacaa tccgctccgc catatggcaa caccattgcc cgccagccag tttccgccag
     1441 cgactttgcc ctgcaattta cgcccggaaa acgctatcgg gtcattgacg tcatccataa
     1501 cgaattgatt acgcactccc actccagcgt ctacagcgaa aatggttttg atcgcgatga
     1561 tgtgtgcttt attgccgtac ttgagcgtta cgggcaacgg ctggctccgg cttgtggttt
     1621 gcttggcggc tttggactga atgaaggtgc gctggctgcg acggtcagcc atgacagcca
     1681 taatattgtg gtgatcggtc gcagtgccga agagatggcg ctggcggtca atcaggtgat
     1741 tcaggatggc ggcgggctgt gcgtggtacg taacggccag gtccaaagtc atctgccgtt
     1801 gcccattgcc ggcctgatga gcaccgacac ggcgcagtcg ctggcggaac aaattgacgc
     1861 cttgaaagcc gccgcccgtg aatgcggtcc gttacccgat gagccgttta ttcagatggc
     1921 gtttctttct ctgccagtga tccccgcgct aaaactaacc agtcaggggc tatttgatgt
     1981 cgagaagttt gccttcacta cgctggaagt cacggaataa taaaaaagcc cggcgtcatg
     2041 ccgggcaaaa gtcaccagtt acgtttatgc cactgtcaac tgctgaattt ttttctcgcg
     2101 gcggattttg cgttcttcca tcaccgccac tatcgccatc aggcagatac aaccaatcgc
     2161 ggcgatatca agtgcggcga aggtgcctgc ccagccggta aggccgaata ccggcgtccc
     2221 atcggcaatc attcccagac ctaacttggc aaagctgtca ccaatcaggt aagcaaaggt
     2281 gcctttaata ccatcggcag cgccaatcgc ttttttaggt acaaagccaa cagcagccac
     2341 accaatcaac aattgcgggc caaagaccag gaaacccaac gcaaagagag aagccagata
     2401 gatatattcg ttactggcat gttgatacac accgagcgtg gcgataatca gcgccagcgc
     2461 gatgcaggcc accaggccac ggcgaccgtt cgccaggtca gagagccagc cccacagcag
     2521 cgtaccgacc agcgcaccag cttcaaacag cgtaaagccc tgaatcgcca ccgctttaga
     2581 gagtttcagt tcctggaacg cgtatacggt tgaccactgg tcgataccaa tacgtaccac
     2641 atagaggaaa atgttggcga agcacagcag ccagatcact ttgtttttca gcacatactc
     2701 aacaaagatc tgccacttgg tcatatcggt agattctgtc tctttgtcct cttcgctgat
     2761 ctcctcgccg aacagttctt cagctttgcc gaggccataa gattccgggg agtcgctgcc
     2821 gtaacgcagg ccgataaaac cgacaatcag cgcgataatc gacgggaaga taaacatgcc
     2881 gatgacatgg ccatcgaaca ggtaatttgc cccgaacagc gccacgcctg ctgcgcctgc
     2941 accgccaagg ttgtgagaaa tattccagaa accgaggaat gtcccgcgtt tacgacgcgg
     3001 cgtccatttg gtgatggtgg agtaactgca cgaaccgccg gtactctgga aaaagccgct
     3061 taaggcgtag aaggcaatca tcaggaacag gctaaccgag ccgctgccca tactggcact
     3121 gaagcccagc atacaaatgg cagacaggat cagcatgaac ggcaggaatt gtttggtgtt
     3181 tttgccgtcg gcgtagtagg aaaccagcgt tttacccacg ccataagtga tggagaaacc
     3241 caggccgatc atccccagct gcgtcatgct caacccgtag gtcgagatca tgtcgttctg
     3301 cgcgatgtta aagttcttgc gaatcaggta catcgtcagg tagccgataa agaccaccag
     3361 gtaggattgc atgaacggtt tgaaccacat tttgcgccgc acttcgagcg gaaggtccag
     3421 ggtcggcttg cgaacctggt ttaagaaagc cagcatgggt tactcctgaa atgaatacct
     3481 gcgtgaggca tgcattgtaa aaatcagcag caaatcaggc ctgagatagc gtccaggcaa
     3541 aacctggaaa atttcttagt tttgccggat atgaagtgaa aaggtgagat gcatcacgct
     3601 tcgcgcggtg tctgggcgtt caaaaagggc agtaacagca gtgcggaaat cccggcggcg
     3661 atagagataa ccacaaaaaa tccgctccag tgccaggtat cgagtacttt cgccagcggc
     3721 caaccagcaa gcgacgcccc cagataagca aacaagccga caaaccccgt cgccgcccct
     3781 gccgcctctt tgtgggaaca ctctgccgcc gccataccga ttaacatctg tgggccaaag
     3841 acaaaaaaac caatggtgaa gaagcaggtt gcctgcatca cgtagctggc aaatggcatc
     3901 agccacaggg agccgactga aagcaaaatt ccggcggcga aaatcaaatt catcggccct
     3961 cggttgccgt taaacaattt gtccgagccc caacccgcta ccagcgcacc gataaatcca
     4021 cccagttcaa acatcgtcac tgccgtattc gccgtgacca gatcgacgcc cagcgtctcg
     4081 gacatataca aattgcccca gtcgttgatc gccgcccgga ccacatagac cagcacatag
     4141 caaaacgaaa gcagccagat atacggattc agcaacacat atttggtgag gatctcttta
     4201 cgcgtcagcc ctgccccttc ttgttgttga gctatctcca gcgcgtcgtg tcgccattca
     4261 ccgaccgccg gtaaacctaa cgcctgcggg cgatcgcgca gccgccagca gagaaaaatt
     4321 cccacgacga tcgccataca accagcaatc atcatcccgg cacgccagcc gtaatgtagt
     4381 gcagccgctg ccatcacaat gggaatgagt gcgccgccga cgttatgcgc cgtgttccat
     4441 aatgcccacc aaccaccgcg ctcggtacgt gaataccagg ccgttaacag acgcgcacac
     4501 accggtgaac cccagccctg gaaaaaggcg ttcagcaccc agagtacggc aaacgcccat
     4561 agcgacgtcg agaagccaaa cagaatgttg ataatgcccg tggcgataag ccctatcccc
     4621 ataaaataac gggcatttga gcgatcgctg acaatgccgg agacaaactt cgacacgcca
     4681 taggtaatgt aaaacagggt cgctaacagg ccgatatcgc tacggctgag cacgccgtta
     4741 gcaaggattt ctggtacggc ggcgttaaaa cttttccgcg tgaagtaaaa cagcgcgtaa
     4801 cccagccaga tggtcagcag aatatgccga cgccagtagc gatagcgggc atcaatttca
     4861 tatttatcag ccattaatgg cgcatcggca ggcgctttca gaaacggcaa catcatcaaa
     4921 ccttagacat agcgttgagg tagagaaacg ctgacacgcg tgccgtgcag acaggaaatg
     4981 tgtaatgtgc cacccagcgc cgttacgcgc tcgcgcattc cggtgaggcc aaaaccttgt
     5041 tgcccggaac ccggcggcaa accgctgcca tcgtcttcaa taaccagcat caaccgttca
     5101 tcctgctgcc agccttgcag ggtgaccgcg ctggcatcag catgtttcac aatgttgttc
     5161 agcccttcct ggcagacacg aaacagcgtc acgcgctggt tttcgcttaa cgctgattca
     5221 tcgattcgcc attcgagatg gctgacaata ccgcgccctt ccagctccat ttcccgcatc
     5281 agtgagcgga tggcctgctc cagggtgaga tcatccaact ggcgcggacg taaccgaccc
     5341 aacaaacggc gcaccgcgtc gtaaacgccc agcgatagtt gttcgatgag ttgcccgctc
     5401 tgcttcacgc tggcgttatc tgccgccagc cgctgaacaa tgcccgcctg agtacgaata
     5461 gcagtgatgg tctggccgat atcatcatgc agctcacgcg ccacatcacg gcgcacgctc
     5521 tcttcggttt ccagcaaccg ttcagccaga tgctgattgc gcgccagttc cttttgcagc
     5581 gactggttaa gttcacgcaa ccgctggatg ccagcgccaa gcaacaaccc tgtcagactt
     5641 tgcaccagca gcgagagcaa taaatccacc ggatgatcgc gccaggtttg actggcgatc
     5701 agcgcgatgg cgttcatcaa cgtcgcaatc agcgcccctt gccaaccata gtgccaggcc
     5761 agcgcgataa tcggcagcac cagacagaat ggcgtaaagc gcgacagttc gtccggcaat
     5821 cccaactgga gccagagact gataacaaac agcagcaagt accagaccag atgtcgcccg
     5881 cgccagttga ttggctgaga aaccagtgac gggccgagcg gcagccaggt gttattggcg
     5941 agatagtgcc agaacaccag acatatcggg gccagcgtca ggccgccagt taaagtcagc
     6001 aacagcgcat tccacgactc tttgccgtgc caaagccagg gcagcgactg caacaacgcc
     6061 gccgccgtta acgccgcccc ctgtaacagc aaggtgcgcc agtcacgctg atggcgatag
     6121 cgcgagatca gcgctacggg cagtaacgtc agtaaactac cgatcatcaa taacggaaaa
     6181 tgggttaaac cgaccgcctg cgttagccag taaatcagca gccactccgc acccagcaat
     6241 acgggccagt atccgcgcgg gcattgcagc attagcccca gacgcagacc aaacggaaat
     6301 aacagcaccg ccatatcagg gcgctcaacc agatgcaggc tgatactcca caggcaaaac
     6361 catgcggcag agaagataaa aaagcaggca ataacggtaa ttaagcggga gaacaacgtc
     6421 ttcatcacca gccatcaaac atgcggcgcg ccagctctac gtcgttactg acgcccagtt
     6481 tttccatcag attggcgcga tggacgtgta ccgttttcgg tgacaagccc agttcggcgg
     6541 caatctcttt caccgccatt ccttgcgcca gtttttccgc cacctggcgt tcacgtttgg
     6601 ttagcgggtc ctgacgaccg gatgccagtt taatggcaat atccggcgtc agataacagc
     6661 cacccgtggc aaccgtatgc accgcagcaa tgagttcatc cgggctacag cgtttggaaa
     6721 gaaagccgcg tgcccccgcg ttaagcgcct gctcaaccag cgcaggactg tcgtgaacgg
     6781 agagcataat cgtcgccata cctttcggca gctggcttag cagctccaga ccggagatat
     6841 cgggcatgga gatatcgcaa atacacacct gcacaccgcg ccccggcagc cccgccagcg
     6901 cctcgcgccc cgaaccaaac tcggcaacta cctgcaaatc aggttccagc cccagcagct
     6961 gcgcaaagcc ggagcggacg atgaggtgat cgtctataag ggcaacggtg atcatggtct
     7021 tgtcctggcg ggtaaaaaaa aacgcgctta ccttaacgat aagcgcgatg ttgttcaagc
     7081 cttgagcggt tactgaaaaa acaccgcgat cttgttaaac atcgtcggat cggactgatt
     7141 acgctgcact ttcacgacat cttccagctt atcgatttgg cttatcatct gctccagacg
     7201 ctggtcgtca ttgaccagta gccagatatg gcttttgtcg ctgtcctgaa tcggcagaca
     7261 aagaatgcct tcaacgttaa aagcgcggcg ggcaaaaagg ccacaaacgt gggtcattac
     7321 gcccggatgg ttgcgaacgg tgagctccag aattacgttg tcatgagttg tgttttgcat
     7381 ggcttattcc cccaccattt cagtattcgc cgcacctggc ggcaccatcg ggtaaacttt
     7441 ttcttcggca tcaatgcgca catggatcag cgccgggcta gggcgattga tgatttcctg
     7501 caatgcagcc tgcggatcgg cttcgttatt caaatcacag gtttcgaggc cgaatccggc
     7561 ggcaatctgc ataaagttga ttttgcccgg ataggtggcg gcaaaaacgc cttgctcgta
     7621 gaacagactc tgttgctgat gcaccagccc cagcgcttcg ttgttcatca gaatgatttt
     7681 gacatccagc tgattttcac tggcggtcgc catctcctga atattcatca tcaggctgcc
     7741 gtcgccggag aaacacaaca ctttgcgatc cgggttcgcc agcgccgcgc caatcgccgc
     7801 aggcaggcca aaacccatcg tgcccagccc accggaggtc agccactggc gtgggcgatt
     7861 gagcggataa gcttgcgcgg tccacatctg atgctgaccc acatcggtgg tgataatcgc
     7921 attgtcatcg acacaggcgg caacggcgtt gatcaggccg taatggctta atggatcgca
     7981 cgctttcggg attggacacg ggaattcgcg ctgcaaatcc gctaccaact ggtgccactc
     8041 tgcacgcggt tgcgcttcca cctgcgggat caattgcgcc agcacgtcat caacatccgc
     8101 ctgaatcgcc acatgcggct gcttgatttt acccagctct gcgcgatcga tatcgacatg
     8161 aataattttg gcattcggac agaactgctc ggttttgcca atcgcccggt catcaaaacg
     8221 cgcaccgagc acaatcaaca aatccgcctc ctgcaaaata tagttggtac tgcgcacgcc
     8281 gtgcatcccc agcataccca gcgacaacgg atgcgctttt ggcagcatgc ccagcgccat
     8341 taaagtcatg gtggtaggca gttgcgcttt ctccgccagt tcacgcaccc gcgcaggcgc
     8401 attgatcaca ccaccaccca gataaagcac cgggcgtttg gcagcgttaa tcattgtagc
     8461 tacgtcacga atgctttctt cgctaaaggc gggtgcagcg gctttttctg ccaccgcggg
     8521 ctgagcttca atctcaaaaa ccgccgtttg tacatcctta ggaatgtcta tccacaccgg
     8581 gccagggcgg cctgattgcg caatgcggaa ggcatcgctc atgacctgcg ggagttcttc
     8641 gatatgtctg accagatagt tgtgtttggt gatggggata gagatgccgt aggtgtcgac
     8701 ttcctggaag gcgtcggtgc cgatcatcga ggcgggaacc tgaccagtga tgcaaatcag
     8761 cgggatggag tccagccgcg catcggcaat ggcggtcacc aggttagtcg cacccggtcc
     8821 gctacaggcc atacagaccg ccggtttacc gtcggtgcgc gccattccct gagcgataaa
     8881 gcccgcgccc tgttcatgac gggccagaat atggcggatt tgcgtgcttt ggcttaaggc
     8941 atcgtaaaca ggcaggatag aaccgcccgg aatgcctgtc acaatcttaa tgccctgctg
     9001 ttccaggaaa tgaacgataa attctgcgcc ggtaaagcgc ttacgcgtca atgttgtgcc
     9061 cgaacttgcc atgctccagt ccttttcttc tgggccgact ttccgggagg tgcttaaacg
     9121 aaaaaccccg cccggtttgc gccgacgggg ttttggaatc gtgtgttgtt ccagtcccta
     9181 cggcgcattg ccgacgacca ccaccacacg cacgacgacc actgcggcgg atggcgcagt
     9241 tggtagtagt tttgcgttga acatggaagt agtcattggg gaccttgttg gttttgtgtt
     9301 taacaatatt tatacaagca cagctttaca ggggagacaa tggaaaattt ttcagcaagg
     9361 gaaaattgag gggttgatca cgttttgtac tgaattgcag ataacaaaaa accccgccgg
     9421 agcgaggttt cgtcagtcgc ccgcggctgg taaccgcaaa gcacactgta ttatgtcaac
     9481 acagaaagta tacgtgttcc gcgcagaacg cgcaatttcg gcacgaattt tgacgtattt
     9541 agtgcatagt tgagtatcga tcacagtttg cgttttgtcc aaatattgct gtttatttat
     9601 acagtaaact tctataatat cactgtacgc aatgtgttat gcgggggccg catcgttacc
     9661 cggcgcacta agtcctggct gaaacgggtg gtgccgtcag cgccttaacc ccgcgtgagc
     9721 acactgtgtt atgtcaacaa gcacaaagtt tctccttgag ataccgcgtg cacaacagct
     9781 ggcaacaggc agcggaaagg tacgtcagct ggcagtgctc ctgaaccaca ggagacgcgt
     9841 atgagcctgg tggatatcgc cattcttatc ctcaaactca ttgttgcagc actgcaactg
     9901 cttgatgctg ttctgaaata cctgaagtaa ttcagattca agtcgcacca aaggggagcg
     9961 ggtaaccgct ccccttttat atttagcgtg cgggttggtg tcggatgcga tgctgacgta
    10021 tcttatccgc cctaccatct ctcccggcaa catttattgc cgcttttgtt tacatattct
    10081 gccgctaaac aactccccat tcctggcgta tatctggcta acattcatca atgtgataga
    10141 ttcctctccc gcatttatgg gaatgcgtag tgacttactc taattatttt tataaaagca
    10201 tccgtgataa tgaaaaggca tagaaacgtc aatttgttat tgatgttggt attactcgtg
    10261 gccgtcggtc agatggcgca aaccatttat attccagcta ttgccgatat ggcgcgcgat
    10321 ctcaacgtcc gtgaaggggc ggtgcagagc gtaatgggcg cttatctgct gacttacggt
    10381 gtctcacagc tgttttatgg cccgatttcc gaccgtgtgg gtcgccgacc ggtgatcctc
    10441 gtcggaatgt ccatttttat gctggcaacg ctggtcgcgg tcacgacctc cagtttgacg
    10501 gtattgattg ccgccagcgc gatgcagggg atgggcaccg gcgttggcgg cgtaatggcg
    10561 cgtactttgc cgcgagattt atatgaacgg acacagttgc gccatgctaa cagcctgtta
    10621 aacatgggga ttctcgtcag tccgttgctc gcaccgctaa tcggcggtct gctggatacg
    10681 atgtggaact ggcgcgcctg ttatctcttt ttgttggttc tttgtgctgg tgtgaccttc
    10741 agtatggccc gctggatgcc ggaaacgcgt ccggtcgatg caccgcgcac gcgcctgctt
    10801 accagttata aaacgctttt cggtaacagc ggttttaact gttatttgct gatgctgatt
    10861 ggcggtctgg ccgggattgc cgcctttgaa gcctgctcca gcgtgctgat gggcgcggtg
    10921 ttagggctga gcagtatgac ggtcagtatt ttgtttattc tgccgattcc agcagcgttt
    10981 tttggcgcat ggtttgccgg acgtcccaat aaacgcttct ccacgttaat gtggcagtcg
    11041 gttatctgct gcctgctggc tggcttgctg atgtggatcc ccgactggtt tggcgtgatg
    11101 aatgtctgga cgctgctcgt tcccgccgcg ctgttctttt tcggtgccgg gatgctgttt
    11161 cctctggcga ccagcggcgc gatggagccg ttccccttcc tggcgggcac ggctggcgcg
    11221 ctggtcggcg gtctgcaaaa cattggttcc ggcgtgctgg cgtcgctctc tgcgatgttg
    11281 ccgcaaaccg gtcagggcag cctagggttg ttgatgacct taatgggatt gttgatcgtg
    11341 ctgtgctggc taccgctggc gacgcggatg tcgcatcagg ggcagcccgt ttaagcgcac
    11401 gtcaccgcag catcgtcatc agctccatgg gagaacgatg ctgctttatc agatcacgca
    11461 tcacccgcat atgcggtgcg gagtaagaat aaaacgcctg atagcccgca caaagcacgc
    11521 tgtctggcgt ctcctgccga gcgtgcgggc acaggcgatg ccagtcgcac gcctggcagt
    11581 cacagcgcgg ttgcgggttt tcgctgccac accatttttg taaggtggcg tcaaaaagct
    11641 caatcgagat acgactgata tctgcccgca cccaacactc gaacagggca attaaaaatt
    11701 tcccccacag ctctgctgtc accgaagcgg ccgtcagatc gccccggtca tcaaattcaa
    11761 gatagggaat gagctgtacg gagcggatgc cccgctgttt gaaattgtga taaaaatcgg
    11821 caggttgcag agcatccttg tactctacaa ccagtttatg cctgtcctct tcagcatcaa
    11881 tgacctgact tcctgtcatg cgactctctc aataaatacg ataagttgat gtatatgcgt
    11941 tacagcaaac agtattgcga gagataacac cgcgagggag atcaaaaatt tgtcacggac
    12001 tacgtgaaat tgggaaaaat cgctattcgt gacatccatt aaattgcgat tacgggtgta
    12061 gtgccagagg atcagcgcca cgatggcgag aatgccaatc gaaatccaga ataacatgcc
    12121 cgcctggtgc cagttgtgtt tgatagccaa cgccatcaac gcgccgtagc ccagcatggt
    12181 acgaaaccac gccagtgatg tgcgctccgg ctgcagtcct gggtcggcaa tgcgtctggc
    12241 tttgcggcta tccggcatac aacaccagtc ccataacgat caccgccacg accatcaata
    12301 ttaagctaat gattaataag ctgttggtgt agggcaggtc ctctttcaga cgcatcgctt
    12361 tttcattgcg taaccagcgc aggtagccat acattgctaa cccgccggaa aacagacaca
    12421 acaacaacgc cagcagttca cgaatgacgg gcgtggcgaa atctggcgca agttgatcaa
    12481 gcccaacccc cgccgccaga aatcctagtg cggtacggat ccacgccaga aaagtacgct
    12541 cattagccag cgagaagcgg taatcgggcg cttctccaag gcgggaaatt ttcattgttg
    12601 ctcctttgca atttcactaa ctctctacct ttagcggttt tttttaagaa tttcgtccgc
    12661 ccgtttcctg atatgctttt gccatccgct acaaggaggt cagatgggta tcatagcgca
    12721 aaataaaatc agttcattgg gcatgctgtt cggtgccatc gctttaatga tggggattat
    12781 ccacttttca ttcggccctt tttccgcacc accgccaacg cttgaaagca tcgtggcgga
    12841 caaaacggct gaaattaaaa gaggtcttct cgccggaata aaaggcgaaa aaattaccac
    12901 cgtggagaaa aaagaggacg tagatgtcga taaaatcctc gatcaaagcg gtatcgcact
    12961 ggccattgct gcgctacttt gcgcatttgt tggcggtatg cgtaaggaaa atcgctgggg
    13021 cattcgcggc gcgctggttt ttggcggcgg aacgctggct ttccatacct tgctttttgg
    13081 tatcggcatc gtgtgcagca ttttgctgat attccttatc ttttccttcc tgaccggcgg
    13141 ttcgctggtt taagcagcct gaataatgcc cgccgggtga tcatcagcgg gcattatcgt
    13201 cagaacttct gttttttctc ttccaccttc accccttgtg tcggtaggcc ggtgtcatag
    13261 tcacgtacca ccggcgaata gccatcttgt gggcgtggac gaaatgcccc catccagcgc
    13321 ggccgtgcat ctttacgcca cggacgcagg ctccattgtt gacatcctcc acgccctgaa
    13381 ggacgtggcc ccctgctacg ttcaggctgt cgcctgaatc atttcggtgg gttcctgctt
    13441 caacgagcgg cctgactgca ccatctctcc acaggcaaga acggcgtgcc ccgccgctaa
    13501 aatgttacga gcgccattta catcggcgtt cgctgtatat ccacatacct ggcatctgaa
    13561 tttactttgt gacaggcgat tttctttcgc tgtatgacca cagcacacgc aacgctggct
    13621 tgtgtacgct ggcggaacag caagcacctg accaccacgc cagagctgct tgtacgcaag
    13681 ctggcggcgc atttcatacc agccctgatc cagtatcgaa cggtttaaac ctgattttgc
    13741 ccggacatt
//
LOCUS       DAJTZL010000114        13499 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.114, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000114 DAJTZL010000000
VERSION     DAJTZL010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13499
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.114"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..650
                     /gene="livJ"
                     /locus_tag="OO544_002833"
     CDS             <1..650
                     /gene="livJ"
                     /locus_tag="OO544_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HCR6768383.1"
                     /translation="KYILEKVKPQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFD
                     GITAGEKDFSTLVARLKKENIDFVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANV
                     SLSNIAGESAEGLLVTKPKNYDQVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQA
                     GLNQSDDPAEIAKYLKANSVDTVMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(849..1232)
                     /gene="panM"
                     /locus_tag="OO544_002834"
     CDS             complement(849..1232)
                     /gene="panM"
                     /locus_tag="OO544_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HCR6768384.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            1656..2765
                     /gene="livK"
                     /locus_tag="OO544_002835"
     CDS             1656..2765
                     /gene="livK"
                     /locus_tag="OO544_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HCR6768385.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            2813..3739
                     /gene="livH"
                     /locus_tag="OO544_002836"
     CDS             2813..3739
                     /gene="livH"
                     /locus_tag="OO544_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HCR6768386.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            3736..5013
                     /gene="livM"
                     /locus_tag="OO544_002837"
     CDS             3736..5013
                     /gene="livM"
                     /locus_tag="OO544_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HCR6768387.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDSASDVR
                     WQWVFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVS
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTFFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAILLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            5010..5777
                     /gene="livG"
                     /locus_tag="OO544_002838"
     CDS             5010..5777
                     /gene="livG"
                     /locus_tag="OO544_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HCR6768388.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRNQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            5779..6492
                     /gene="livF"
                     /locus_tag="OO544_002839"
     CDS             5779..6492
                     /gene="livF"
                     /locus_tag="OO544_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HCR6768389.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            6776..7840
                     /gene="ugpB"
                     /locus_tag="OO544_002840"
                     /pseudo
     CDS             6776..7840
                     /gene="ugpB"
                     /locus_tag="OO544_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
     gene            7938..8825
                     /gene="ugpA"
                     /locus_tag="OO544_002841"
     CDS             7938..8825
                     /gene="ugpA"
                     /locus_tag="OO544_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HCR6768390.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSRQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALHSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            8822..9667
                     /gene="ugpE"
                     /locus_tag="OO544_002842"
     CDS             8822..9667
                     /gene="ugpE"
                     /locus_tag="OO544_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HCR6768391.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            9669..10739
                     /gene="ugpC"
                     /locus_tag="OO544_002843"
     CDS             9669..10739
                     /gene="ugpC"
                     /locus_tag="OO544_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HCR6768392.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTEGDIWINDQRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVRDPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLTGRVNNEGTHFELDGGIVLPLNGGYRQYAGRKM
                     TLGIRPEHIALSSQAEGGVPLVMDTLEILGADNLAHGRWGEQKLVVRLAHQERPTAGS
                     TLWLHLAENQLHLFDGETGQRV"
     gene            10736..11479
                     /gene="ugpQ"
                     /locus_tag="OO544_002844"
     CDS             10736..11479
                     /gene="ugpQ"
                     /locus_tag="OO544_002844"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCR6768393.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(11466..11906)
                     /locus_tag="OO544_002845"
     CDS             complement(11466..11906)
                     /locus_tag="OO544_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HCR6768394.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            12026..>13499
                     /gene="ggt"
                     /locus_tag="OO544_002846"
     CDS             12026..>13499
                     /gene="ggt"
                     /locus_tag="OO544_002846"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HCR6768395.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDINKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNIYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTV"
BASE COUNT         3010 a         3452 c         3838 g         3199 t
ORIGIN      
        1 cgaaatatat tcttgagaaa gtgaaaccgc agcgtattgc tatcgttcac gacaaacagc
       61 aatacggcga aggtctggca cgagcggtgc aggacggcct gaagaaaggc aatgctaacg
      121 tggtgttctt tgatggtatc accgccgggg aaaaagattt ctctacgctg gtggcgcgtc
      181 tgaagaaaga gaatatcgac ttcgtttact acggcggtta ccacccggaa atggggcaaa
      241 tcctgcgtca ggcacgcgcg gcagggctga aaactcagtt tatggggccg gaaggtgtgg
      301 ctaacgtttc gctgtctaac attgcgggcg aatcagcgga agggctgctg gtgaccaagc
      361 cgaagaacta cgatcaggtt ccggcaaaca aacccattgt tgacgcgatc aaagcgaaaa
      421 aacaggaccc aagcggcgca ttcgtttgga ccacctacgc cgcgctgcaa tctttgcagg
      481 cgggcctgaa tcagtctgac gatccggctg aaatcgccaa atacctgaaa gcgaactccg
      541 tggataccgt aatgggaccg ctgacgtggg atgagaaagg cgatctgaaa ggctttgagt
      601 tcggcgtatt tgactggcac gccaacggca cggcgacaga tgcgaagtaa tcattaatcg
      661 gcaactttgg gttgccgcca aattgctaat atcgagtacg ttgcttcatg ccggatgcgg
      721 cgtaaacgcc ttatccggcc tataagatcc aaagaaatca gtaaattgca acacacattg
      781 taggcctgat aagcgtagcg catcaggcaa tttctcgttg gtcatcactt ttgaaatcaa
      841 cttgacgatt aacgcttctc ccagccgccc tgttgcgccg taaatcccag tgcctgcata
      901 aacgctgtca tcacaccgcg atcttccacg cctgcatccg ccatccacca gcatgaaacg
      961 ccaggattgt tacgcaaaac ctcttccagc agatattgcc ccacaccgcg acgtcgggtg
     1021 acttcccgca cacgcaggga atccagtgct ccctcggtgc cgcttaaggt tacccgtacg
     1081 gcagcgagca ggcgctcgtt aaaacgcgcg gcgtagatac ggtggttatc gtcaacctgt
     1141 aaagaggaag gggaatactc cggccagatc ttttgcaggt caatccggtc ctggtcgcta
     1201 aagttttcta atcgaatgat ggtcagcttc atgggtaacc cgtgtaaatc acaaaagtgg
     1261 aaccagtgta gcgaaataat ttaatcggag gctttctctt ttttatttct tttggcaggt
     1321 gattaatttt ttaacagcaa taattacaaa gttaaaacat tagagaatga aaaataccca
     1381 gcataatccc ctgaatggta gtgaattatt ccgccctttg tgccgttatt ttatgctgac
     1441 aaaggcactt ttttctgttt atctatcaat aaattcagaa tattatctgt tcttaatcga
     1501 ctgaaaaata gggattttaa tcactgttat cacaaaatac tgcgctaaac ccttaatcag
     1561 acaggcaaaa acagtgcagt ataaaaaaag aacagtctga tttgttaaca cataaaaaca
     1621 aagcaacaca acatcacgaa tggggatttt tgactatgaa acggaatgcg aaaactatca
     1681 tcgcagggat gattgcactg gcaatttcac acaccgctat ggctgacgat attaaagtcg
     1741 ccgttgtcgg cgcgatgtcc ggcccgattg cccagtgggg cgatatggaa tttaacggcg
     1801 cgcgtcaggc gattaaagac attaatgcca aagggggaat taagggcgac aagctggttg
     1861 gcgtggaata tgacgacgcc tgcgacccga aacaagccgt tgcggtcgcc aacaaaatcg
     1921 ttaatgacgg cattaaatac gttattggtc atctgtgttc ttcttctacc caacctgcat
     1981 cagatatcta cgaagacgaa ggtattctga tgatctcgcc gggagcgacc aacccggagc
     2041 tgacccaacg cggttatcaa cacattatgc gtactgccgg gctggactct tcccaggggc
     2101 caacggcggc aaaatacatt cttgagacgg tgaagcccca gcgcatcgcc atcattcacg
     2161 acaaacaaca gtatggcgaa gggctggcgc gttcggtgca ggacgggctg aaagcggcta
     2221 acgccaacgt cgtcttcttc gacggtatta ccgccgggga gaaagatttc tccgcgctga
     2281 tcgcccgcct gaaaaaagaa aacatcgact tcgtttacta cggcggttac tacccggaaa
     2341 tggggcagat gctgcgccag gcccgttccg ttggcctgaa aactcagttt atggggccgg
     2401 aaggtgtggg taacgcatca ttgtcgaata ttgctggcga tgctgccgaa ggcatgttgg
     2461 tcactatgcc aaaacgctat gaccaggatc cggcaaacca gggcatcgtt gatgcgctga
     2521 aagcagacaa gaaagatccg tccgggcctt atgtctggat cacctacgcg gcggtgcaat
     2581 ctctggcgac tgcccttgag cgtactggca gcgatgagcc gctggcgctg gtgaaagatt
     2641 taaaagctaa cggtgcaaac accgtgattg ggccgctgaa ctgggatgaa aaaggcgatc
     2701 ttaagggatt tgattttggt gtcttccagt ggcacgccga tggttcatcc acggcagcca
     2761 agtgatcatc ccaccgcccg tgaaatgcgg gcgggtttag aaaggttacc ttatgtctga
     2821 gcagttttta tatttcttgc agcagatgtt taacggcgtc acgctgggca gtacctacgc
     2881 gctgatagcc atcggctaca ccatggttta cggcattatc ggcatgatca acttcgccca
     2941 cggcgaggtt tacatgattg gtagctatgt ctcctttatg atcatcgccg cactgatgat
     3001 gatgggcatt gataccggct ggctgctggt ggcggcggga ttcgtcggcg caatcgtcat
     3061 tgccagcgcc tacggctgga gtatcgaacg ggtagcctat cgcccggtgc gtaactctaa
     3121 gcgcctgatt gcgctcatct cggcaatcgg catgtccatc ttcctgcaaa actacgttag
     3181 cctgaccgaa ggttcgcgtg acgtggcgct gcccagtctg tttaacggtc agtgggtggt
     3241 ggggcatagc gaaaacttct ctgcctctat taccaccatg caggcagtga tctggattgt
     3301 tactttcctc gccatgctgg cgctgacgat tttcattcgt tattcccgta tggggcgcgc
     3361 ctgccgtgcc tgcgcggaag atctgaaaat ggcgagcctg cttggcatca acaccgaccg
     3421 ggtgattgct ttgacctttg ttattggcgc ggcgatggcg gcagtggctg gcgtactgct
     3481 cggtcagttc tacggcgtca ttaaccccta tatcggcttt atggccggga tgaaagcctt
     3541 taccgctgcg gtgctcggtg ggattggcag cattccaggg gcgatgattg gcgggctgat
     3601 cctggggatt gcggaggcgc tctcttctgc ctatctgagt acggaatata aagatgtggt
     3661 ctcattcgcc ctgctgattc tggtgctgct ggtgatgccg accggtattc tgggtcgccc
     3721 ggaggtagag aaagtatgaa accgatgcat attgcaatgg cactgctctc tgccgcgatg
     3781 ttctttgtcc tcgctggcgt ctttatgggc gtgcaactgg agctggatgg caccaaactg
     3841 gtggtcgaca gcgcttcgga tgtccgttgg cagtgggtgt ttatcggcac ggcggtggtc
     3901 tttttcttcc agcttttgcg acctgctttc cagaaagggc taaaaagcgt ttccgggccg
     3961 aagtttattc tgcccgcgat tgatggctcc acggtgaagc agaaactgtt cctcgtggcg
     4021 ctgctggtgc ttgcggtgtc gtggccgttt atggtgtcac gtgggacggt ggatatcgcc
     4081 actctgacca tgatctacat tatcctcggt ctcgggctga atgtggttgt tggtctttct
     4141 ggtctgctgg tgctggggta cggcggtttt tacgccatcg gcgcttacac ttttgcgctg
     4201 ctcaatcact attacggtct ggggttctgg acctgtttgc cgattgccgg actgatggca
     4261 gcggcggcgg gcttcctgct tggttttccg gtgttgcgtc tgcgcggtga ttatctggcg
     4321 atcgttaccc tcggtttcgg cgaaattgtg cgcatattgc tgctcaataa caccgaaatt
     4381 accggcggcc cgaacggtat cagtcagatc ccgaaaccga ctttcttcgg ccttgagttc
     4441 agccgtaccg cccgtgaagg cggttgggac acgttcagta atttctttgg cctgaaatac
     4501 gatccctccg atcgcgtcat cttcctgtat ctggtggcgt tgctgctggt ggtgctaagc
     4561 ctgtttgtca ttaaccgcct gctgcggatg ccgctggggc gtgcgtggga agcgttgcgt
     4621 gaagatgaga tcgcctgccg ttcgctgggc ttaagcccgc gtcgtatcaa gctgactgcc
     4681 tttaccatca gtgccgcgtt tgccggtttt gccggaacgc tgtttgctgc gcgccagggc
     4741 tttgtcagcc cggaatcctt cacctttgct gaatcggcgt ttgtactggc gatagtggtg
     4801 ctcggcggta tgggatcgca gtttgcggtg attctggcgg caattttgct ggtggtatcg
     4861 cgcgaattga tgcgtgattt caacgaatac agcatgttaa tgctcggtgg tttgatggtg
     4921 ctgatgatga tctggcgtcc gcagggcttg ctgcccatga cgcgcccgca actgaagctg
     4981 aaaaacggcg cagcgaaagg agagcaggca tgagtcagcc attattatct gtgaacggcc
     5041 tgatgatgcg cttcggcggc ctgctggcgg tgaacaacgt caatcttgaa ctgtacccgc
     5101 aggagatcgt ctcgttaatc ggccctaacg gtgccggaaa aaccacggtt tttaactgtc
     5161 tgaccggatt ctacaaaccc accggcggca ccattttact gcgcaatcag cacctggaag
     5221 gtttaccggg gcagcaaatt gcccgcatgg gcgtggtgcg caccttccag catgtgcgtc
     5281 tgttccgtga aatgacggta attgaaaacc tgctggtggc gcagcatcaa caactgaaaa
     5341 ccgggctgtt ctctggcctg ttgaaaacgc cttctttccg tcgcgcccag agcgaagcgc
     5401 tcgaccgcgc cgccacctgg cttgagcgca ttggtttgct ggaacacgcc aaccgtcagg
     5461 cgagtaacct ggcctatggt gaccaacgcc gtctggagat tgcccgctgc atggtgacgc
     5521 agccggagat tttaatgctc gacgaaccgg cggcaggtct taacccaaaa gaaaccaaag
     5581 agctggatga gctgattgcc gaactgcgca atcatcacaa caccactatc ttgttgattg
     5641 aacacgatat gaagctggtg atggggattt cagaccgaat ttatgtggtc aatcagggga
     5701 cgccactggc aaacggtacg ccggagcaaa tccgtaataa cccggacgtg atccgtgcct
     5761 atttaggtga ggcataagat ggaaaaagtc atgttgtcct ttgacaaagt cagcgcccac
     5821 tacggcaaaa tccaggcgct gcatgaggtg agcctgcata tcaatcaggg cgagattgtc
     5881 accctcattg gcgcgaacgg ggcggggaaa accaccttgc tcggcacgtt atgcggcgat
     5941 ccgcgcgcta ccagcgggcg aattgtgttt gatgataaag acattaccga ctggcagacg
     6001 gcgaaaatca tgcgcgaagc ggtagcgatt gtcccggaag ggcgtcgcgt cttctcgcgg
     6061 atgacggtgg aagagaacct ggcgatgggc ggcttttttg ccgaacgcga ccagttccag
     6121 gagcgcataa agtgggtgta tgaactgttc ccacgtctgc atgagcgccg tgttcagcgg
     6181 gcaggcacca tgtccggcgg tgagcaacaa atgctggcga ttggtcgcgc gctgatgagc
     6241 aacccgcgtt tgctgctact tgatgagcca tcgcttggtc ttgcgccgat tatcatccag
     6301 caaattttcg acaccatcga gcagctgcgc gagcagggga tgactatctt cctcgtcgag
     6361 cagaacgcca accaggcgct aaagctggcg gatcgcggct acgtgctgga aaacggccat
     6421 gtggtgctct ccgatactgg tgatgcacta ctggcgaatg aagcggtgag aagtgcgtat
     6481 ttaggcgggt aataacacgt tgattgataa ggagtcaaaa gactcctttg agacaggtga
     6541 caaatgtaaa attgcctgat gcgctgcgct tatcaggcct actgggtgag ttgcaatata
     6601 ttgaatttgc acgatcttgt aggccggata aggcgtttac gccgcatccg gcatgaaacg
     6661 atgagtaatc tgtagagttt gattcagacc ttctattttt ccgcttatcc gtgccccatc
     6721 tcccattttc cctcacccac gccgtcaccg ccttgtcatc tttctgacac cttacttgca
     6781 ggtttatgaa gttggcaccg ccaccatgat ggcatcgaaa gccattaaac cggtatatga
     6841 cgtgtttaaa gaggcaggga ttcaattcga tgagtcgcag tttgtgccga cggtttccgg
     6901 ttactactcc gacagcaaaa cgggccactt actctcccag ccattcaaca gctcgactcc
     6961 cgttctctat tacaacaaag acgccttcaa gaaagcgggg ttagacccgg aacagccgcc
     7021 gaaaacctgg caggatctgg cggactcttc cgcaaaactg aaagcctccg gtatgaagtg
     7081 cggctacgcc agcggctggc agggctggat ccaactggaa aactttagcg cctggaacgg
     7141 tctgccgttt gccagcaaaa acaacggctt tgacggcacg gacgcggtgc tggagttcaa
     7201 caagccggag caggtgaaac acattgccat gctcgaagag atgaacaaga agggcgactt
     7261 cagctacgtc ggtcgtaagg atgaatccac cgagaagttc tataacggtg attgcgcgat
     7321 gaccaccgcc tcttccggtt ctctcgctaa tattcgtgag tacgccaaat ttaactatgg
     7381 cgtaggcatg atgccttacg acgccgatgc gaaagatgca ccgcaaaacg ccattatcgg
     7441 cggggccagc ctgtgggtga tgcagggcaa aaataaagaa acgtataccg gcgtggcgaa
     7501 gttcctcgac ttcctcgcga agccagaaaa cgctgccgag tggcatcaga aaaccggtta
     7561 tctgccaatc accaaagcag cgtatgacct gacccgtgag cagggctttt atgagaaaaa
     7621 cccaggggcg gataccgcga cgcgtcagat gctgaataag ccgccgttgc cgttcaccaa
     7681 agggctgcgt ctgggcaaca tgccgcagat ccgcgtgatt gtggatgaag agctggagag
     7741 cgtgtggacc ggtaagaaga caccacagca ggcactggat accgccgttg agcgtggaaa
     7801 tcagttgctg cgccgctttg agaaatcgac gaagtcttaa tcagtgtaat gtcggatgcg
     7861 tttcgcttat ctgacctggc atcgcgtgta ggccggataa gcgaagcgca tccggcacag
     7921 ttcaggaatt aaccgtaatg tcatcatccc gtccggtgtt ccgctcgcgc tggctgcctt
     7981 atctgctggt cgcgccgcag ctcatcatca ccgttatctt ttttatctgg cctgcgggcg
     8041 aagcgttgtg gtactcgcgg caaagcgtcg atccgtttgg tttctccagc cagtttgtcg
     8101 gtctggataa cttcgtcacg ctgttccatg acagctacta tctagactcc ttctggacga
     8161 cgataaaatt cagcaccttt gtcaccgtca gcggtttgct ggtatcgctg ttctttgcgg
     8221 cgctggtgga gtacatcgtg cgcggcagcc gtttctatca aaccttgatg ctgctgccgt
     8281 atgccgtggc tcccgccgtt gccgccgtat tgtggatctt cctgtttaac cccggtcgcg
     8341 ggctgatcac ccattttctc gctgagttcg gctacgactg gaaccacgcg caaaacagcg
     8401 gtcaggcaat gtttctggtg gtgtttgcct cggtgtggaa gcaaatcagc tacaactttc
     8461 tgttcttcta tgccgcgctg cactccattc cccgttcgtt gattgaagcc gcagctattg
     8521 acggcgcagg gccgattcgc cgcttcttta agattgctct gccgcttatc gctccggtga
     8581 gtttcttcct gctggtggtg aacctggtgt acgccttctt cgacaccttc ccggtgatcg
     8641 acgccgccac gtccggcggg ccggttcagg ccaccactac gctgatttat aagatctacc
     8701 gcgaaggctt taccggactg gatctggctt cgtctgccgc gcagtcggtg gtgttgatgt
     8761 tcctcgtcat cgtgctgacg gtggtgcagt tccgctatgt tgaaagcaag gtgcgttacc
     8821 aatgattgag aaccgtccgt ggctgacgat attcagccat accatgctga tcctcgggat
     8881 cgcggtgatc ctcttcccgc tgtacgtggc gtttgtcgcg gcgacgctgg ataaacaggc
     8941 cgtctatgcc gcgccgatga cgctcatccc cggcacacat ctgctggaaa atatccacaa
     9001 catctgggtt aacggggtag gcacgaatag cgcgccgttc tggcggatgt tgcttaacag
     9061 ctttgtgatg gcgttcagca ttacgctcgg caaaattacc gtctcgatgc tctcggcatt
     9121 tgccattgtc tggtttcgtt ttccgctacg taacctcttc ttctggatga tttttatcac
     9181 cctgatgctg ccggttgaag tacgtatctt cccgacggtg gaagtcatcg ccaacctgaa
     9241 aatgctcgac agctacgccg gtttaacgct gccgctgatg gcctcggcga ccgccacgtt
     9301 tctgttccgc cagttcttta tgaccctacc ggatgaattg gtggaagccg cgcggatcga
     9361 cggcgcgtca ccaatgcgtt tcttttgcga catcgttttt ccgttgtcca aaactaatct
     9421 ggcggcgctg tttgtgatca cctttatcta cggctggaat cagtatttgt ggccgttgtt
     9481 gattattacc gacgttgatc tcggcaccac cgtggcaggg atcaaaggga tgatcgctac
     9541 aggcgaaggc accacggaat ggaactcggt aatggcggcg atgttgttaa cgcttatccc
     9601 tccggtggtg attgttttag tgatgcagcg tgccttcgtg cgcggcctgg tcgatagtga
     9661 gaaataagat ggcaggactg aaattacagg cagtaaccaa aagctgggat ggcaaaaccc
     9721 aggtcattaa accgctgacc cttgatgtgg cggatggcga atttatcgtg atggtcgggc
     9781 cgtctggctg cgggaaatcg acgctgctgc gcatggttgc cgggctggag cgggtgacag
     9841 aaggcgatat ctggatcaac gaccagcgcg tgaccgaaat ggagccgaaa gatcgcggga
     9901 ttgcgatggt gttccagaat tacgcgcttt atccgcatat gagcgtagag gagaacatgg
     9961 cgtgggggct gaaaattcgc ggcatgggca agcagcaaat tgccgagcgc gttaaagaag
    10021 cggcgcgcat tctggaactg gacggtctgc ttaagcgtcg tccgcgtgag ctatccggcg
    10081 gtcagcgcca gcgtgtggcg atgggccgcg cgattgtgcg cgatccggcg gtgttcttgt
    10141 ttgatgagcc gctctctaac ctcgatgcca agctgcgcgt gcagatgcgt cttgaactgc
    10201 aacagttgca ccgtcgcctg aaaacgactt cactctacgt tactcacgat caggttgaag
    10261 cgatgacgct cgcccagcga gtaatggtga tgaacggtgg cgttgccgaa cagattggca
    10321 caccagttga agtctacgaa aagcccgcca gcctgtttgt ggcgagtttt atcggcagcc
    10381 cggcgatgaa cctgctgaca ggccgcgtga ataatgaagg cacgcatttc gaactggacg
    10441 gcggtatcgt gctaccgcta aacggtggct accgtcagta tgccgggcgt aaaatgactc
    10501 tcggcattcg cccggaacat atagcgctaa gctcgcaggc agaaggtggt gtgccgctgg
    10561 tgatggacac gctggagatc ctcggcgcag ataacctggc gcacggacgc tggggcgagc
    10621 agaagctggt ggtgcgactg gcgcatcagg agcgcccgac ggcaggcagc acgctgtggc
    10681 tgcatttggc ggaaaatcag ctgcatcttt ttgatggtga aacaggacaa cgagtatgag
    10741 taactggcct tatccccgca tcgtcgctca tcgtggcggc ggtaagctgg ccccggaaaa
    10801 caccctggcg gcaatcgacg tcggggcaaa atacggtcat aagatgatcg aatttgacgc
    10861 gaagttatcg aaagatggcg agatcttcct gctccatgac gacaatctcg aacgcaccag
    10921 caacggctgg ggcgtcgcgg gtgaactgaa ctggcaggat ttactgcgcg tggatgcggg
    10981 cagttggtac agcaaagcgt ttaaaggtga gccgctgccg ttgctttcgc aggtggcgga
    11041 acgctgtcgc gaacacggga tgatggcgaa tatcgaaatc aaacccacca ctggcactgg
    11101 accattaacg ggcaaaatgg tggcgctggc ggcacgccaa ctgtgggccg gtatgacgcc
    11161 gccgctgctg tcatcgtttg agattgatgc tttagaagct gcacaacagg cggcaccgga
    11221 actgccgcgt ggtttattgc ttgatgagtg gcgcgacgac tggcgcgaac tgaccgcgcg
    11281 gctgggctgc gtctctattc atctcaatca taagttactc gataaagcgc gagtgatgca
    11341 gttgaaagac gccggattac ggattctggt ttataccgtc aataaacccc agcgcgcggc
    11401 agagttgctg cgctggggcg tggattgcat ctgtaccgat gcgattgacg tgattggtcc
    11461 gaactttacg gcccaatagt tttcaacgga atgtcaggct gcggcgtgct gggctgattc
    11521 agcatgtcgc cgtttctcct ttccggcagc atatgctgct gattcaacga actatccgga
    11581 ttacggttgc tgtttaacat cccgccgttg gtgttgggca gcatttgctg ccgggcggga
    11641 tttcgctccc ccggctgtga ctgcaacacc cgctgagaat tattgtttat ctggttttct
    11701 aaatgctgct gttgcaactg agtttgcgtt ttcagttgct gattcagcat ccctttttgc
    11761 tggatttgct gagtctgcat ctgggtttgc atccgctgct ggctgggtat ctgatacccc
    11821 ggctggttag ggttgttcag agtattaatg ggctgtgcaa agccgacaaa cggcaggagt
    11881 gccgtaagaa tcagaagtcg tttcatcgcg tatcctcctc tgaagatatc ctttaagttt
    11941 actcgcttcc cgacaaaacg atgattaatt cagagttata taccaggctt agctggggtt
    12001 gccccttaat ctctggagaa taacgatgat aaaaccgacg tttttacgcc gggtggccat
    12061 tgctgctctg ctctcaggaa gttgttttag cgccgccgcc gcgcctcctg cgccgcccgt
    12121 ctcgtatggt gtggaggaag atgtcttcca cccggtacgc gcgaaacagg gaatggtagc
    12181 gtctgtggac gccactgcca ctcaggtggg ggtggatatc ctcaaggagg gcgggaatgc
    12241 cgttgatgcc gccgtggcgg tgggctacgc gctggcggta acgcatccgc aggcagggaa
    12301 tctgggcggt ggtggtttta tgttaatccg ctcgaaaaat ggcaatacca cggctatcga
    12361 tttccgcgaa atggcacccg ccaaagcgac ccgcgatatg ttcctcgatg atcagggcaa
    12421 cccggacagc aaaaaatcac tcacttcgca tctggcttcc ggcacaccgg gtacggtagc
    12481 aggtttctcg ctggcgctgg ataaatacgg caccatgccg ctgaacaaag tcgtgcagcc
    12541 cgcgtttaaa ctggcacgcg atggttttat cgttaacgac gcgctggctg acgatctcaa
    12601 aacctacggt agcgaagtgt tgccgaatca cgaaaacagt aaagctatct tctggaaaga
    12661 gggcgagccg ctgaaaaagg gcgacacgct ggtgcaggcg aacctggcaa agagcctgga
    12721 gatgattgct gaaaacggcc cggacgaatt ctataaaggc acgattgcgg aacagatcgc
    12781 ccaggagatg cagaaaaacg gtggcttgat cactaaagaa gatttagcgg cctataaagc
    12841 ggtcgaacgc actccgataa gcggcgatta tcgcgggtat caggtttact ccatgccacc
    12901 gccatcctcc ggcgggatcc atatcgtaca aatcctcaat attctggaaa acttcgatat
    12961 gaagaaatac ggctttggca gcgccgatgc gatgcaaatc atggcagaag cggagaaata
    13021 cgcctacgcc gaccgctcgg aatatcttgg cgacccggat tttgtcaaag tcccgtggca
    13081 ggcactgacc aataaagcct atgccaaatc cattgccgat caaattgata tcaacaaagc
    13141 gaagccgtcc agtgagattc gccctggcaa gcttgcgcct tatgagagta atcaaactac
    13201 ccattactct gtagtggata aagacggtaa cgcggtggcg gtgacctata cgctgaacac
    13261 caccttcggt acgggcattg tcgcgggcga gagcggtatt ctgcttaata atcagatgga
    13321 tgatttctcc gccaaaccgg gcgtaccgaa catttacggg ctggtgggcg gtgatgccaa
    13381 cgccgtcggg ccgaataaac gtccgctgtc atcgatgtca ccaaccattg tggtgaaaga
    13441 cggtaaaact tggctggtta ccggtagtcc tggcgggagt cggattatta ccaccgtgc
//
LOCUS       DAJTZL010000115        13465 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.115, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000115 DAJTZL010000000
VERSION     DAJTZL010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13465
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.115"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            62..784
                     /locus_tag="OO544_002847"
                     /pseudo
     CDS             62..784
                     /locus_tag="OO544_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908053.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            794..1273
                     /locus_tag="OO544_002848"
     CDS             794..1273
                     /locus_tag="OO544_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6768396.1"
                     /translation="MRLHVKLKEFLSMFFMAILFFPAFNASLFFTGVKPLYSIIKCST
                     EIFYDWRMLILCFGFMSFSFLNIHVILLTIIKLFLIKKTKVVNFATDITIQLTLIFLL
                     IAIVIAPLIAPFVTGYVNANYHPCGNNTGIFPGAIYIKNGMKCNNEYISRKEDSAVK"
     gene            complement(1410..2195)
                     /locus_tag="OO544_002849"
     CDS             complement(1410..2195)
                     /locus_tag="OO544_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6768397.1"
                     /translation="MNLRRVCCICVLFSLLAGCVSESSIDEKKKKAQVTQSNINKNTP
                     QQLTDKDLFGNETTLAVSEEDIQAALDGDEFRVPLNSPVILVQSGNRAPETIMQEEMR
                     KYYTVSTFSGIPDRQKPLTCNKNKDKNENEDVASAENMNWMQALRFVAAKGHQKAIIV
                     FQDTLQTGKYDSGLKSIVWSDYKNQKLTDTMSLRYLVRFTLVDVATGEWAAWSPVNYE
                     YKVLPPLPDKNEASTTDMTEQQIMQLKQKTYKAMVKDLVNRYQ"
     gene            complement(2525..2601)
                     /locus_tag="OO544_002850"
     tRNA            complement(2525..2601)
                     /locus_tag="OO544_002850"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2565..2567),aa:Asp,seq:gtc)
     gene            complement(2734..3465)
                     /gene="dnaQ"
                     /locus_tag="OO544_002851"
     CDS             complement(2734..3465)
                     /gene="dnaQ"
                     /locus_tag="OO544_002851"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HCR6768398.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVEKKGGSCLWRA"
     gene            3530..3997
                     /gene="rnhA"
                     /locus_tag="OO544_002852"
     CDS             3530..3997
                     /gene="rnhA"
                     /locus_tag="OO544_002852"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HCR6768399.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(3994..4716)
                     /locus_tag="OO544_002853"
     CDS             complement(3994..4716)
                     /locus_tag="OO544_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR6768400.1"
                     /translation="MKPARVPQTVVAPDRWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLRETDRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            4750..5505
                     /gene="gloB"
                     /locus_tag="OO544_002854"
     CDS             4750..5505
                     /gene="gloB"
                     /locus_tag="OO544_002854"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HCR6768401.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAIAANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTASQMYQSLNKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINVINEETLLQQPEERFAWLRSKKDRF"
     gene            5577..6935
                     /gene="mltD"
                     /locus_tag="OO544_002855"
     CDS             5577..6935
                     /gene="mltD"
                     /locus_tag="OO544_002855"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HCR6768402.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(6984..7580)
                     /locus_tag="OO544_002856"
                     /pseudo
     CDS             complement(6984..7580)
                     /locus_tag="OO544_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
     gene            complement(7611..8411)
                     /locus_tag="OO544_002857"
     CDS             complement(7611..8411)
                     /locus_tag="OO544_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCR6768403.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            8652..9566
                     /locus_tag="OO544_002858"
     CDS             8652..9566
                     /locus_tag="OO544_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6768404.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            complement(9563..10366)
                     /gene="dkgB"
                     /locus_tag="OO544_002859"
     CDS             complement(9563..10366)
                     /gene="dkgB"
                     /locus_tag="OO544_002859"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HCR6768405.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
     gene            complement(10529..10605)
                     /locus_tag="OO544_002860"
     tRNA            complement(10529..10605)
                     /locus_tag="OO544_002860"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10569..10571),aa:Asp,seq:gtc)
     gene            complement(10785..10900)
                     /gene="rrf"
                     /locus_tag="OO544_002861"
     rRNA            complement(10785..10900)
                     /gene="rrf"
                     /locus_tag="OO544_002861"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(10995..>13465)
                     /locus_tag="OO544_002862"
     rRNA            complement(10995..>13465)
                     /locus_tag="OO544_002862"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT         3544 a         3302 c         3034 g         3585 t
ORIGIN      
        1 gtgtagatct cggtggtcgc cgtatcatta aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
       61 aaaaaaaata ctcagcccaa taacatcatt tgattacatt cattttatca ctacacatcc
      121 ttcaggtata aaagatacac tcgcctggtt agttaacgcg agtaaattaa tgactgagtt
      181 tgatgacaat gggaaaatta tatttaacat taatgcctta aagtatacaa aagcaagtta
      241 ctttgaaata cttggagaga aatatataaa aatcacaaca tccagcccct ggcttttaga
      301 aaagctcggt aaatatattt tctcttcacg agcgcctcaa gttctggaac tcgctattgg
      361 ctggcgagga gctttatctg aatcaataaa aggagttaag ttctgtatct ggttttcagt
      421 agcctggaga accatagaat ttattatgag ttcagagcga gatctggtta attttcttgg
      481 cgatttttca atggatgtgg ccaaagcagt tatagccggt ggtgttgcaa ccgctattgg
      541 aagcctggct tcttttgcct gtgttagctt tggctttcca gtaattcttg tcggaggagc
      601 aattttactg acagggatag tgtgtacagt tgttttaaat gaaatcgatg ctcaatgcca
      661 tttatcagaa aaattaaaat atgcaattag agatggacta aaacggcaac aggaacttga
      721 taaatggaaa agggaaaaca tgactccatt tatgtatgtt cttaacactc cacccgtgat
      781 atgaggatta accatgcgtt tacatgttaa attaaaagag tttctttcga tgttttttat
      841 ggccatatta tttttccctg cattcaatgc ttctttgttt tttaccggag ttaaacctct
      901 atattcaata ataaaatgct ccacggaaat attttatgat tggagaatgt tgattctgtg
      961 ctttggtttc atgtcgtttt cctttttaaa tattcatgta attttactta cgataataaa
     1021 attattctta atcaaaaaaa caaaggtagt caatttcgcc acagacataa caatacagct
     1081 aactcttata tttttactaa tagctatcgt aatagcacct ttaatagctc cttttgtcac
     1141 aggatatgtc aatgctaatt atcacccctg cggtaataat acgggaattt tcccaggagc
     1201 aatctatatt aaaaatggca tgaaatgtaa taatgaatat atttctcgca aggaagattc
     1261 agcagttaaa taattaaaca ttctaaaaca aacagttttt aactccggat tccatgtcct
     1321 ttcttgcact gtctttgtca agaaaaattt cttatgcaaa gaattttgta ttatttccgc
     1381 cagaaattcc tggcggcaga tataatttat tattgatagc ggttaaccaa atcttttacc
     1441 atcgctttat aggtcttttg cttaagttgc atgatttgct gctctgtcat atcagtcgta
     1501 ctggcttcat tcttgtcggg caatggtgga agcactttat attcataatt caccggcgac
     1561 caggcagccc actcacctgt agctacatcc accagcgtga atcgtaccag atagcgcagt
     1621 gacatcgtgt cagtcaattt ttgattttta taatctgacc aaactattga tttcaatcca
     1681 gagtcatatt ttcctgtctg caacgtatcc tggaaaacaa taatcgcttt ctgatgccct
     1741 tttgcagcca caaaacgcag tgcctgcatc cagttcatat tctcagcact ggcaacatcc
     1801 tcgttctcat ttttatcttt gtttttgtta caagtcagag gcttttgcct gtccgggata
     1861 ccagagaatg tggaaacagt ataatatttt cgcatctcct cctgcataat ggtttccggt
     1921 gcgcggttgc cggattgaac aagtattaca ggagaattaa ggggaacgcg gaactcatct
     1981 ccatcaagcg cagcttgaat atcctcttcg gatacggcca gagtggtttc attaccgaat
     2041 aaatctttgt ctgtcagttg ctggggagtg tttttattaa tattactttg tgtgacttga
     2101 gcttttttct tcttttcatc aatagaagat tcagagacac atcctgcaag cagcgagaat
     2161 aatacacata tacaacaaac ccttcttaaa ttcataatcg gacactccct cgcctgtcat
     2221 aatcatggta atgtttagct aacgattttt aattatatac tccacttgaa tcagaacaaa
     2281 caaaaataac agtatatata aattcattaa atcaatagaa aaatgaaaat cataacattc
     2341 aaatatattt aaattgaatg atttgatagt atgattaaat attaatgata tggtctacca
     2401 tatgtgatta acacatttac agcataacaa aaccatagac cgaagaaata gcgggattta
     2461 aatgcaaaaa aaccgcaaaa ttaaaaatct tgcggttctc tgaactcatt ttcatgagtg
     2521 aatttggcgg aacggacggg actcgaaccc gcgcccccct gcgtgacagg caggtattct
     2581 aaccgactga actaccgctc cgcgttgtgt tccgttggga acgaggcgaa tattacggat
     2641 tgcctcaccc ttcgtcaacg gtttttctca tcttttgaat cgtttgctgc aaaaatcgcc
     2701 caagccgcta tttttagcgc cttttacagg tatttatgcc cgccagaggc agcttccgcc
     2761 cttcttctcc accagatcaa gacgggcttc atgagctgca agctcttcat ctgtcgcaaa
     2821 aacaacgcgt aacttacttg cctgacgtac aatgcgctga attgttgctt caccttgttg
     2881 ctgctgtgtc tctccttcca tcgcaaaagc catcgacgtt tgaccaccgg tcatcgccag
     2941 ataaacttcc gcaaggatct gtgcatcgag taatgccccg tgtagcgttc gtttactgtt
     3001 atctatttcg tagcgggcac ataacgcatc gaggctgttg cgcttaccag gaaacatttt
     3061 cctcgccacc gcaaggctat cggtgacctt acagaaggta ttggtcttcg gaatatcgcg
     3121 cttaagcaac gaaaactcgt agtccataaa gccgatatcg aacgctgcgt tatggatcac
     3181 caactccgcg ccgcggatat agtccatgaa ctcatcggct acttcggcaa acgtgggctt
     3241 atcgagcaaa aattcatcgg caataccatg tacgccaaag gcttccggat ccaccagccg
     3301 atcgggtttg agataaacat ggaagttatt gcccgtcagg cgacggttca ccacttcaac
     3361 ggcaccaatc tcaatgatct tgtggccttc atagtgcgca ccaatctggt tcataccggt
     3421 ggtttcggta tcgagaacga tctggcgtgt aattgcagtg ctcatagcgg tcatttatgt
     3481 cagacttatc gttttacagt tcgattcaat tacaggaagt ctaccagaga tgcttaaaca
     3541 ggtagaaatt ttcaccgatg gttcgtgtct gggcaatcca ggacctgggg gttacggcgc
     3601 tattttacgc tatcgcggac gcgagaaaac ctttagcgca ggctacaccc gcaccaccaa
     3661 caaccgtatg gagttgatgg ccgctattgt cgcgctggag gcgttaaaag aacattgcga
     3721 agtcattttg agtaccgaca gccagtatgt ccgccagggt atcacccagt ggatccataa
     3781 ctggaaaaaa cgtggctgga aaaccgcaga caaaaaacca gtaaaaaatg tcgatctctg
     3841 gcaacgtctt gatgctgccc tagggcaaca tcaaatcaaa tgggaatggg ttaaaggcca
     3901 tgccggacac ccggaaaacg aacgctgtga tgaactggct cgtgctgcgg caatgaatcc
     3961 cacactggaa gatacgggct accaagtcga agtttaagct tgtggtttac gacattgccg
     4021 ggtggctcca accgcctggc gaattcgtgg cttgttttta ctctgtttca tcgggtttag
     4081 cgttaaagga atagtccgtt tccgggcaac aataagttgt aagcaaccaa gcgcaggaat
     4141 atgcgcattc aatagttttc ctccgtgttt gttccacggg agaacgtgga aacggctggc
     4201 gtgcagcact tcaaaattca acaaagagag ccagtccagt tgccgcatca gagtaaacat
     4261 ccggctgtta tagggcgagg tttttcgcaa taccggcaca agtttgcgta atcccattaa
     4321 actaatggga ttgaagccac taatcaccag ccagccatca tcaatcaata cccgatcggt
     4381 ttcacgcaat aaacgatgcg gatcggtgca ccacggcaat gtatgtgcca gtagacaaac
     4441 atcaacggat ttatcggcaa aaggaagatg aagtggatcc gcctgcacct ggacgggcat
     4501 tccttgcgca gaaacattca cttgatgaga aaccgcgcac gcttcgcaat tgatttctgc
     4561 gcttaaattg ccaatcttaa gcagatgaaa accatacatt ttagtgaacc acgggttgag
     4621 ctggcgctcc agcgccttgc gataaagctc tccccagggc aaatcgcccc agcgatcagg
     4681 agccacgaca gtttgaggga ctcttgccgg tttcatcaca accttccgtt tcacactaag
     4741 aggtaatcta tgaatcttaa cagtattccc gcctttgatg acaattacat ctgggttttg
     4801 aatgatgaag caggtcgctg cctgattgtc gatcccggag acgcagagcc agtattaaac
     4861 gccattgccg ccaataactg gcaaccggag gccatatttc tcacccacca tcatcacgat
     4921 cacgttggcg gcgtaaaaga actggtggaa aagtttccgc aaattgtggt gtatggtcca
     4981 caagagacac aagataaggg aacaacacag gtagtcaaag atggcgaaac tgccttcgtt
     5041 ttggggcatg aatttagtgt aattgccaca ccgggtcaca ctttaggaca tatctgttac
     5101 ttcagtaaac cttatctatt ttgcggcgac acgctgtttt ctggtgggtg tggtaggttg
     5161 tttgaaggga cagcatcaca aatgtatcaa tcacttaata agttaagtgc gttacctgac
     5221 gatacattgg tatgttgtgc tcatgaatat acgttatcaa atatgaagtt tgctttgagt
     5281 attcttccgc acgatttgtc cataaatgat tattatcgta aagttaagga gttacgggca
     5341 aaaaatcaaa taacactacc cgtaattctg aaaaatgagc ggcaaattaa tgttttttta
     5401 agaacggaag atattgattt aattaatgta attaatgaag aaacattatt gcaacaacct
     5461 gaagagcgtt ttgcatggtt aaggtcaaag aaagataggt tctgataaaa ctttcttgtc
     5521 atcggctccg ttcgccgtta tgatcggtcg tcttttaagc aactattgac acacacatga
     5581 aggcaaaagc gatattactc gcctctgtcc tgctcgtggg ttgccagagt accggcaacg
     5641 ttcaacagca cgcacagagc ctttctgcag ctggtcaagg ggaagcagca aagtttacaa
     5701 gtcaggcacg atggatggac gatgggacgt ctatcgcgcc agatggtgac ttgtgggctt
     5761 tcattggcga cgagctaaag atgggaattc cggaaaatga ccggattcgc gaacagaaac
     5821 agaaatattt acgcaataag agctatctcc acgatgtaac tttacgggca gagccgtata
     5881 tgtactggat agcagggcaa gttaaaaaac gtaacatgcc tatggaactg gtactactac
     5941 ccatagtgga gagcgctttt gatcctcacg caacgtctgg cgccaatgcc gcaggcatct
     6001 ggcagatcat tccgagcacg gggcgcaatt atggtttgaa acagacccgc aattatgacg
     6061 cgcgtcgcga tgttgttgct tcaacaactg ccgcgctgaa tatgatgcag cgtctgaaca
     6121 aaatgtttga cggcgactgg cttctgaccg tagcggctta taacagcggc gaaggtcggg
     6181 tcatgaaggc aattaaaacg aacaaagcgc gtgggaaatc cacggacttc tggtcgttac
     6241 cgttgccgca ggaaacgaag cagtacgtgc ctaaaatgct ggcattgagt gatattctca
     6301 aaaacagcaa gcgttatggc gtacgtctgc caacgaccga tgaaagccgt gctctggcgc
     6361 gtgtgcacct gagtagcccg gttgaaatgg cgaaggttgc agatatggcg gggatttccg
     6421 tcagcaagct gaagacattc aacgctggcg tgaaaggctc cacgctgggc gcaagtgggc
     6481 cgcagtacgt gatggtgcca aagaagcatg cagatcaact gcgtgaatct ctcgcttcag
     6541 gcgaaattgc tgctgtacag tcgacgctgg ttgccgacaa tacgccgctt aacagccgtg
     6601 tttacaccgt acgctctggc gacacgcttt caagcatcgc ttcacgtctc ggcgtaagca
     6661 ccaaagattt gcagcagtgg aacaaactgc gcggatctaa gctgaagcca ggccaaagtt
     6721 tgacgattgg tgcaggcagt agcgcacagc ggttggcaaa caacagcgat agcattacgt
     6781 atcgtgtgcg caaaggcgat tcgctttcaa gcattgctaa acgccacggc gtgaacatca
     6841 aagatgtgat gcgctggaac agcgacactg cgaatctgca accaggcgat aagctgacgt
     6901 tgtttgtgaa aaacaacaac atgccagatt cctgacaaac cagataataa aaaaggcacc
     6961 gattcccccg gtgccttttt tatttatgcc gctttatgtg cttctaccat gatgatatca
     7021 ctggtgaaag agccatcagt ctgcaaggaa aaatacgttc tcacctctgt cgatgcgctc
     7081 tgttggtaaa tgcgaatagc gtctactaac gcttctggcg tacgcattct cacgacccat
     7141 gaagaaaatt ccagcggtaa cttatctgta attaaattat caactatcag attggcttca
     7201 ttgattaacg tcaaccacgc accgctggcg tagtttcgta cgtgagaggt atcgcgtaat
     7261 gcttctaccg cctgtaacca gatgtcgcgc actgggtgac ccggagacat tacatccatc
     7321 acaatcagcc taccgccagg tttcaatatc ctattcactt ctcgcagtgc tgcaccaaca
     7381 tcatgccagt gatgggcaga ataacggctg ataacaatat caaatgcgtt atcggcaaat
     7441 ggcagacttg cggcatatcc ctggcgggtg gtgatatttt tcagttgccg ggcttcggcg
     7501 gcttgtgcca cgacatccag catgtgggca gataagtcat acgccaccac cgcgctcacg
     7561 ttttgcgcag cgccaaacgt tggtctgccc tgcggcagac ctgacatacc ttatttatca
     7621 ggcttgccgg gactgaattc aacgagtagc ggattgtgat cggaagcgcg cgtaaccagt
     7681 acagaagctt cactgacgtt cagaccacgg tagaaaacaa aatcgagcgg gcgaccaaac
     7741 gcccgacggc gctgatcatc ggtaaaacgc acctggcgca gcgacatttc ccgcgcaaag
     7801 cgatataacg cgttcatcct tctacggctc caggcattga aatctcccgc cataatgacc
     7861 gggccgctgt ggtgagctat ctgatcacca ataggaagta actgcttact atagacatcc
     7921 acgcccagac tgaagttgac ggcgtgtata ttaaccacca tcaacaggcg ggtgtcaggt
     7981 aatggataga ccgtcaccag tgccgacttc gccagacgca aaatgggttc tcgttcacgt
     8041 aacgggcagc aatacactgg atgtgccgcc gaaagggtca ttacgccaga aggatgttgt
     8101 ggcagcacga aagcgggtac ctgatcggcg gcaagatagt tagcggtcgc aaactgtacc
     8161 aactctggcg ttgtttgcgc ttcctgcaat aacaccagat gtgcgtcttt gccgtaattc
     8221 tttaataccg acaaccattc agcgcgttgc tgtttgtata tgttccacac caggatccga
     8281 atacgctctt cggtacttaa cggttctccg ggtggtaatg cctggccgat gctcgcaaaa
     8341 gaccccggcg gtaagatcct ctccgcaggt tgtccggcaa catagcgcat ggcataggtg
     8401 ttttttcgca cttttgactt tgaaacctct attacgtcaa cccttccgca ctcgggaggg
     8461 ttctactgtt atagggattt tagctcactc tttcaacgag caattgtata ttgttatgta
     8521 agcaagtgct tacaccggtc agaaatttgc tcattattga gaacattaaa tcggatgaca
     8581 tatcacgcat cctgatggtt taatgacaaa taaattcaaa atatctcatc tattttgcaa
     8641 caggagcaaa aatgaaagcc acgtcggaag aactcgccat ttttgtttcg gtcgtcgaaa
     8701 gcggcagctt tagccgggca gcggaacaat tagggcaagc aaactcagcg gtaagccggg
     8761 cggtgaaaaa gctggaaatg aaacttggcg ttagcctgct taatcggacc acgcggcaac
     8821 ttagcctgac ggaagaaggc gagcgttatt ttcgtcgcgt acagtcaatt ttgcaggaga
     8881 tggcagcggc agaatcagaa attatggaga cgcgtaatac accgcgtgga ctgttacgga
     8941 tcgatgccgc aactccagtg gtgctgcact ttctgatgcc gttaattaag cctttccgtg
     9001 aacgctatcc ggaagtcact ttatcgctag tctcctccga aacgattatt aatttgatcg
     9061 aaagaaaagt ggatgtcgcg atacgcgctg gtacgttaac ggactccagc ttacgtgcca
     9121 ggccgttatt taacagttat cgaaaaatta tcgcctcccc cgattatatt tcccgctacg
     9181 ggaagccaga aacgatcgac gatttaaagc aacatgtttg cctgggattc actgaacccg
     9241 cttccctcaa tacctggccg atagcctgta gtgatggaca attacatgag gtgaagtacg
     9301 gtttgtcatc caatagtggg gaaacactga aacagctttg cctgagtggc aacgggattg
     9361 cgtgtttgtc cgactacatg atcgacaaag aaatcgctcg cggagaattg gtggagttaa
     9421 tggcagataa agtgttgcca gtggaaatgc cattcagtgc cgtctattac agcgaccgtg
     9481 cggtaagtac gcgcatccgg gcttttatcg atttccttag cgagcatgta aaaacagctc
     9541 ccggaggagc tgtcagagag gcttaatccc attcaggagc cagaccttcc gggctaacca
     9601 ggcggtcgtt gcaatccagt gcggcgatcg cttttttatc ttcggcatca agctgtaaat
     9661 tttgtgcctt aagattactt tccaggtttt tacgtttagt agaagaagga attactgagt
     9721 aaccttcccc catagcccac gccagaatca cttgtgccgg agtcgcattg tgtttagctg
     9781 caatacgagc aataacctca tctttcagtg ccttaccata agccagcgtc atataggaag
     9841 tgatatgaat accgtgctgt ttagcccagg caaccacttt acggttttgc agataaggag
     9901 agagttcaat ctggttggta gcgatgtttt cagcgccaac agcagcaatc gctttttcca
     9961 tcaacgggat cgtgaagttg gaaataccga tctcacgtgt cagaccttgt tttttggcct
    10021 ccagcagcgc ctgcataaac tcttcaacag agacttcatc gtttggtgac ggccagtgga
    10081 ttagcgtcag atcaacataa tcggtacgca atttttgcag gctctctttt agactcggga
    10141 tcaatttgtc tttgctgaga ttttcaatcc agattttagt ggtgatgtag agttcatgac
    10201 gtggcacgcc actttctgca atcgcctgac ctactgcggc ttcgttatca tagatttgtg
    10261 cagtatcaat tgcgcgataa ccaagttcaa gcgccgtttt cacagatgaa ataacaacgt
    10321 cgtctttgag acggaaagta cctaaaccaa atgcagggat agccataata tgcctctttt
    10381 aatgctattt tcgttaactg aggtgattat gggcttcaga ctgttgcgga aaaagagcat
    10441 aaaatgcaga ggatttttgc gattctggca ataatagata tacagacgaa aaaaaacctc
    10501 gagcgttaac tcgaggcttc ttaataagtg gcggaacgga cgggactcga acccgcgacc
    10561 ccctgcgtga caggcaggta ttctaaccga ctgaactacc gctccaccga attcttttac
    10621 aaccaccggt tttatgaccg gcttactgct taatttgatg cctggcagtt tatgaatcac
    10681 ttcgggctgt tgcttcgcaa cgttcaaaag ctttcgcttt tgtcctactc tcttatcgag
    10741 tagtgaactg cctcaaaggt cagtgccatt cttcgtaatt tgatgcctgg cagttcccta
    10801 ctctcgcatg gggagacccc acactaccat cggcgctacg gcgtttcact tctgagttcg
    10861 gcatggggtc aggtgggacc accgcgctac ggccgccagg caaattctgt tttatcagac
    10921 cgcttctgcg ttctgattta atctgtatca ggctgaaaat cttctctcat ccgccaaaac
    10981 atcttcggcg ttgtaaggtt aagcctcacg gttcattagt accggttagc tcaacgcatc
    11041 gctgcgctta cacacccggc ctatcaacgt cgtcgtcttc aacgttcctt caggaccctt
    11101 aaagggtcag ggagaactca tctcggggca agtttcgtgc ttagatgctt tcagcactta
    11161 tctcttccgc atttagctac cgggcagtgc cattggcatg acaacccgaa caccagtgat
    11221 gcgtccactc cggtcctctc gtactaggag cagcccccct cagttctcca gcgcccacgg
    11281 cagataggga ccgaactgtc tcacgacgtt ctaaacccag ctcgcgtacc actttaaatg
    11341 gcgaacagcc atacccttgg gacctacttc agccccagga tgtgatgagc cgacatcgag
    11401 gtgccaaaca ccgccgtcga tatgaactct tgggcggtat cagcctgtta tccccggagt
    11461 accttttatc cgttgagcga tggcccttcc attcagaacc accggatcac tatgacctgc
    11521 tttcgcacct gctcgcgccg tcacgctcgc agtcaagctg gcttatgcca ttgcactaac
    11581 ctcctgatgt ccgaccagga ttagccaacc ttcgtgctcc tccgttactc tttaggagga
    11641 gaccgcccca gtcaaactac ccaccagaca ctgtccgcaa cccggatcac gggtccacgt
    11701 tagaacatca aacattaaag ggtggtattt caaggccggc tccatgcaga ctggcgtcca
    11761 cacttcaaag cctcccacct atcctacaca tcaaggctca atgttcagtg tcaagctata
    11821 gtaaaggttc acggggtctt tccgtcttgc cgcgggtaca ctgcatcttc acagcgagtt
    11881 caatttcact gagtctcggg tggagacagc ctggccatca ttacgccatt cgtgcaggtc
    11941 ggaacttacc cgacaaggaa tttcgctacc ttaggaccgt tatagttacg gccgccgttt
    12001 accggggctt cgatcaagag cttcgcttgc gctgacccca tcaattaacc ttccggcacc
    12061 gggcaggcgt cacaccgtat acgtccactt tcgtgtttgc acagtgctgt gtttttaata
    12121 aacagttgca gccagctggt atcttcgact gatttcagct ccatccgcaa gggacttcac
    12181 ctacatatca gcgtgccttc tcccgaagtt acggcaccat tttgcctagt tccttcaccc
    12241 gagttctctc aagcgccttg gtattctcta cctgaccacc tgtgtcggtt tggggtacga
    12301 tttgatgtta cctgatgctt agaggctttt cctggaagca gggcatttgt tgcttcagca
    12361 ccgtagtgcc tcgtcatcac gcctcagcct tgattttccg gatttgcctg gaaaaccagc
    12421 ctacacgctt aaaccgggac aaccgtcgcc cggccaacat agccttctcc gtcccccctt
    12481 cgcagtaaca ccaagtacag gaatattaac ctgtttccca tcgactacgc ctttcggcct
    12541 cgccttaggg gtcgactcac cctgccccga ttaacgttgg acaggaaccc ttggtcttcc
    12601 ggcgagcggg cttttcaccc gctttatcgt tacttatgtc agcattcgca cttctgatac
    12661 ctccagcatg cctcacagca caccttcgca ggcttacaga acgctcccct acccaacaac
    12721 gcataagcgt cgctgccgca gcttcggtgc atggtttagc cccgttacat cttccgcgca
    12781 ggccgactcg accagtgagc tattacgctt tctttaaatg atggctgctt ctaagccaac
    12841 atcctggctg tctgggcctt cccacatcgt ttcccactta accatgactt tgggacctta
    12901 gctggcggtc tgggttgttt ccctcttcac gacggacgtt agcacccgcc gtgtgtctcc
    12961 cgtgataaca ttctccggta ttcgcagttt gcatcgggtt ggtaagtcgg gatgaccccc
    13021 ttgccgaaac agtgctctac ccccggagat gaattcacga ggcgctacct aaatagcttt
    13081 cggggagaac cagctatctc ccggtttgat tggcctttca cccccagcca caagtcatcc
    13141 gctaattttt caacattagt cggttcggtc ctccagttag tgttacccaa ccttcaacct
    13201 gcccatggct agatcaccgg gtttcgggtc tataccctgc aacttaacgc ccagttaaga
    13261 ctcggtttcc cttcggctcc cctattcggt taaccttgct acagaatata agtcgctgac
    13321 ccattataca aaaggtacgc agtcacacgc ctaagcgtgc tcccactgct tgtacgtaca
    13381 cggtttcagg ttctttttca ctcccctcgc cggggttctt ttcgcctttc cctcacggta
    13441 ctggttcact atcggtcagt cagga
//
LOCUS       DAJTZL010000116        13261 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.116, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000116 DAJTZL010000000
VERSION     DAJTZL010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13261
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.116"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(894..1985)
                     /gene="ychF"
                     /locus_tag="OO544_002863"
     CDS             complement(894..1985)
                     /gene="ychF"
                     /locus_tag="OO544_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HCR6768406.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRVGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            complement(2102..2686)
                     /gene="pth"
                     /locus_tag="OO544_002864"
     CDS             complement(2102..2686)
                     /gene="pth"
                     /locus_tag="OO544_002864"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCR6768407.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            2963..3241
                     /gene="ychH"
                     /locus_tag="OO544_002865"
     CDS             2963..3241
                     /gene="ychH"
                     /locus_tag="OO544_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HCR6768408.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(3296..4975)
                     /gene="dauA"
                     /locus_tag="OO544_002866"
     CDS             complement(3296..4975)
                     /gene="dauA"
                     /locus_tag="OO544_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HCR6768409.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(5099..6046)
                     /gene="prs"
                     /locus_tag="OO544_002867"
     CDS             complement(5099..6046)
                     /gene="prs"
                     /locus_tag="OO544_002867"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HCR6768410.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(6197..7048)
                     /gene="ispE"
                     /locus_tag="OO544_002868"
     CDS             complement(6197..7048)
                     /gene="ispE"
                     /locus_tag="OO544_002868"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HCR6768411.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            complement(7048..7671)
                     /gene="lolB"
                     /locus_tag="OO544_002869"
     CDS             complement(7048..7671)
                     /gene="lolB"
                     /locus_tag="OO544_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HCR6768412.1"
                     /translation="MPQPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            7885..9141
                     /gene="hemA"
                     /locus_tag="OO544_002870"
     CDS             7885..9141
                     /gene="hemA"
                     /locus_tag="OO544_002870"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HCR6768413.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            9183..10265
                     /gene="prfA"
                     /locus_tag="OO544_002871"
     CDS             9183..10265
                     /gene="prfA"
                     /locus_tag="OO544_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HCR6768414.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            10265..11098
                     /gene="prmC"
                     /locus_tag="OO544_002872"
     CDS             10265..11098
                     /gene="prmC"
                     /locus_tag="OO544_002872"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCR6768415.1"
                     /translation="MEYQYWLREAISQLQASESPRRDAEILLEYVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            11095..11487
                     /locus_tag="OO544_002873"
     CDS             11095..11487
                     /locus_tag="OO544_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB family protein"
                     /protein_id="HCR6768416.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            11491..12299
                     /locus_tag="OO544_002874"
                     /pseudo
     CDS             11491..12299
                     /locus_tag="OO544_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
     gene            12335..13189
                     /gene="kdsA"
                     /locus_tag="OO544_002875"
     CDS             12335..13189
                     /gene="kdsA"
                     /locus_tag="OO544_002875"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HCR6768417.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
BASE COUNT         3345 a         3286 c         3394 g         3236 t
ORIGIN      
        1 attcagcgag acgatctgct ttggtattcc cattgtgttg tttgatgccc ataccactcc
       61 tatatagtac ccagactatg ggtctatgga ttttgtcatt ttgtttaaat caatgaataa
      121 tatcctctta tcatcatgat atttgtatgg tgcaatcatg atgagcttct cagagaataa
      181 ttctctatta ccgggcaaaa tcaggaacac aggaattaat atatatttaa catccccctt
      241 acaaaggtga gtttatatcg agttattccc taagttgtcg ttacttattc caattcgtta
      301 ctataaatat ggcaaaaata ttacaacaaa cagcggttaa aaaatttggt gaagattttt
      361 tgtaataatc ctaattagtt atgggaattt cgcttgatgt atctgcataa caaaaacaat
      421 atgcgccatt tttgcattac taataagaaa aagcaaaaaa catcgatatt catttttggt
      481 caatgggttt tctggcctat cgttgttatt gtgctactgg ttatcatttt catcttgcca
      541 ggatcgcacg taatcacgtt attcggggac tttatctgcg ggatattacc cttcaggtaa
      601 gtccttttct cgccagcata ttggagcgtg atcaatattg atcagctgtg aagggggtga
      661 tgtgtagttg aacaaaaact tacgttatat cactactaat gagatatccg tcatctgaca
      721 ctgatgtctt taattattct cgtggttcac tgtaggcaat aagcacaaaa gtgtaggatg
      781 ttacaagaat gattaggact cggtgaaatg aaaaatccac gcaattgcgt ggattttata
      841 tacttttgcg ctcttcatga gatttagcga aacctcatga gacaataaat taattagacg
      901 ttgaaaagga agttcatcac atcgccatct ttaacgatgt aatctttacc ttctgcacgc
      961 attttgcctg cttctttcgc gccttgttca cctttgtaag tgatgaaatc ttcaaacgag
     1021 atggtttgtg cacggataaa gcctttttca aaatcagtat ggattttgcc cgctgcctgc
     1081 ggcgcggttg ctccaaccgg aatggtccat gcacgcactt ctttcacccc agcggtgaag
     1141 taagtttgca ggttcagcag cttataaccg acacggatca cacggttcag gcccggttct
     1201 tccagcccaa gctcctgcat aaactcgtca cgttcttcgt cgtccagttc ggcaatgtct
     1261 gcttcaacag cagcacaaac cggaaccaca acagaacctt ctttcgccgc gatttcacgc
     1321 acctggtcaa gatatgggtt gttttcaaaa ccgtcttcgt tgacgttggc gatgtacatt
     1381 gttggtttca gcgtcaggaa gctcaggtaa cgaatagccg ctttctcttc agcgcttaaa
     1441 tccagcgcgc gcagcatacc tgcgttttcc agctggggca ggcatttttc taggaccgcc
     1501 agctcagctt tcgcgtcttt atcgccacct ttggctttct tctgtacgcg atgaatcgca
     1561 cgttcgcagg tgtcgaggtc tgccagcgcc agttcggtgt tgataacttc aatatcgtca
     1621 gccgggttaa ctttgccgga aacgtgaatg atgttgtcat tttcaaagca gcgaacaacg
     1681 tgaccgatcg cttcggtttc acggatgttg gtcaggaact ggttacccag accttcgcct
     1741 ttcgatgcgc cttttaccag accggcgata tcgacaaatt ccatggtcgt gggaagcgta
     1801 cgttgcggtt ttacgatttc agccagttga tccaggcgag ggtcaggcat tggtacgacg
     1861 cctgtgttcg gctcaatggt gcagaatgga aagttggccg cttcaatacc ggctttggtc
     1921 agcgcgttga acagggtaga tttcccgacg ttgggcaaac cgacgatacc gcatttgaat
     1981 cccatgattt aaatcacctt aatatcttaa taatcaacct gttattgcta acagattgca
     2041 gaaatggaaa taactttgcc tattatacac ggcactcggc aaaaatgccg cagacaacga
     2101 cttattgcgc tttaaaggcg tgcaatcggt tcgttgcttt ggtcaagcca tctgtaaacc
     2161 acatttcagt acaacgcgcc gcttcgtcga tggcttcatc aattaacttc tgttcactaa
     2221 caggcggttt gcctaacaca aaaccgacaa ctttattttt atcgccagga tgaccgattc
     2281 cgatgcgtaa acggtgaaag ttagggttat tacccaattt actgatgatg tctttcagtc
     2341 cattgtgacc accatggcca ccgcccaatt taaatttggc gacgccagga ggcagatcca
     2401 gttcgtcgtg ggccaccaga atttcgtccg gattaatgcg gaaaaaactg gccatcgccg
     2461 caacggcttt gccgctgaga ttcataaatg tagtcgggac taacaggcgg acatcttcgc
     2521 ctccaagagt gactcgcgaa gtataaccaa agaatttagc ctcttcgcgc agcggagcgc
     2581 gcaaacgctc tgccagtaag tcaacgaacc aggcaccagc attatgtcgc gttgcggcgt
     2641 attcagcacc ggggttcgcc aggccgacaa tcaatttaat cgtcacgttt tttgtcctga
     2701 gtgtgtacat aactggcgcg tagtttactg gttgcggccc cgcttgacaa aaaactgcgt
     2761 atcaaatgca gataacgtaa taattgcctg agtggactat tagaaagtca aggtgttcag
     2821 gcgtttattt gtaaagtttt gttgaaataa gggttgtaat tgtgatcacg cccgcacata
     2881 acccacaggg tgttgtctat actttacaca taaggaagag gggtattccc tgttacaacc
     2941 cagaaagttc cggaggtgac atatgaaacg caaaaacgct tcgttactcg gtaacgtgct
     3001 catggggttg gggctggtgg taatggtggt cggcgtgggg tattcaatcc tcaaccagtt
     3061 accacagttt aatatgcccc agtatttcgc acatggtgca gtgctaagta ttttcgtcgg
     3121 tgccattctc tggctggcgg gtgcccgtgt tggcgggcat gaacaggtgt gcgaccgtta
     3181 ctggtgggtt cgtcactatg acaaacgttg ccgccgtagc gataatcgcc gtcatagcta
     3241 acaaataatg cagtttgctg accagtcagt ttgcgctgac tggtcaatct cgtacttata
     3301 aatccgccat cgccgcgcga cgattcggga agaacgcgag gcgtcccggg atcggttgaa
     3361 tgcctgcgcg agccatagtg cgcagtggct ggaattccac gttgcacacg cgcagttcac
     3421 atccttcggg cagacgcttc acaaaacgct ggaacgcatc aagaccacca gcatcaagta
     3481 ccggaacggc atcccacttc agaatcacaa tccgtttgcc ttcaagacgt gactccaggt
     3541 ccgtgaacaa gccttcagca gcagcaaaaa acagcgggcc aataacgcgc agtaccagaa
     3601 catcgtctgg aacatctacg actaccggtg ccaggcgagt catacgtgcg atacgacgca
     3661 taaacagcag cgatgccagc acgatcccca cgctgatggc aataaccata tcaaacagca
     3721 cggtcagcga catgcacagc agcatgacaa tgatgtcatc tttcggtgca tgacgcagca
     3781 agtcgaccac tttatgcgct tcactcatgt tccacgccac catcaacagc agggctgcca
     3841 tagcggaaag cggcagccag gagagcagcg gtgccagtac cagcagggca agaataacca
     3901 gaatagagtg gatcaccgcc gagatagggg aagttgcccc ggcacggacg ttagcggcag
     3961 aacgcgcgat ggcagctgta gcggtaatac caccaaagaa cggagcgata atattcccca
     4021 gtccctgtcc aaccagttcg ctgttcgcct tgtgtttcgt cccggtcata ccatccagta
     4081 ccacggcgca gagcagagat tcgattgcgc cgagcattgc cattgagaat gccgcaggca
     4141 gcagtgtgcg aatagaatcc caggttagcg tgaattctga attaggcaga tcccacggca
     4201 gcaccagttg cggcagcagt tgcggaatac cgttaccctg agaaccatcg gccaggacgt
     4261 agtggaattg cgaaccgatg gtagcaacat gtccgccgag caggttaaca atccccatca
     4321 ccgcgcaacc agccagcaag gccggaaggt gaccgggtaa acgaatgccc agacgcggcc
     4381 aaaaaacaag aatacctagc gtcacaatgc caatggcagc atcacccaca ttaatggtcg
     4441 gcagcgccat aaataatgcg ccgacttttt gtagataatg ttccgggaca tgggccattt
     4501 gcagaccgag aaaatcttta atctgcatgg taccgatggt gatcccgata cccgaggtga
     4561 aacctaaggt gacggaaacc ggaatatact caatcaggcg accaaagcgt gccagaccca
     4621 tcagaatcaa aaagatcccc gacagcaagg tcgcaaccag cagtcctgcc agtccaaact
     4681 gttgcgacac gggatagaga attaccacaa atgccgcagt cggaccggaa acgctaaagc
     4741 gtgacccacc cgtcagagca atgacaatcc ccgcaacagc tgcggtatat aaaccgtact
     4801 ggggtgccac accactacca atagccaacg ccatcgccag cgggatagca ataatcccga
     4861 cggttatccc ggcaatcagg tcacgggtaa accgtgcggc agtatatttt tctttccagc
     4921 aagcgtcgat cagagcgcgg aaaggcatca catgtgagga aaatattttg ttcacaataa
     4981 tgtttcatcc gtgagcgcat catctgtcaa ctaaatggca ggtgaaggag gcataggtca
     5041 tacaaatgga tattacagac aaaaaaaccc gccgcagcgg gtctttgagc gggttcgatt
     5101 agtgttcgaa catggcagag atcgattctt cgttgctgat acgacgaatc gcttcggcca
     5161 gcatacctga cagggtcaga gtacgcacgt tcggcagtga tttgatttca tcgctcagcg
     5221 gaatggtatc gcagacaacg acttcatcaa ttacagagtt acgcaggttg ttcgccgcgt
     5281 tgccagagaa gatcgggtga gtcgcgtacg caaatacacg tttagcacca cgttctttca
     5341 gagcttcagc agctttacac agcgtaccgc cagtgtcgat catatcatcg accagtacgc
     5401 agtcacgacc tgcaacgtca ccgatgatat gcatcacctg ggaaacgttc gcacgcggac
     5461 gacgtttgtc gatgattgcc atatcggtat cgttcagcag cttagcgata gcgcgggcac
     5521 gcacaacgcc gccgatgtcc ggagaaacca caattgggtt atccagattc agctgcagca
     5581 tgtcttccag caggatcggg ctaccaaata cgttatcaac cggaacgtcg aagaaaccct
     5641 gaatctgttc agcgtgcaga tccactgtca gcacacggtc aacaccgacg ctggagagga
     5701 agtctgcaac cactttcgca gtgattggta cacgagcgga acggacgcga cggtcctggc
     5761 gcgcatagcc aaagtagggg ataacagcgg tgatacgacc tgcggaagca cgacgcaggg
     5821 catcaaccat aacgactaat tccatcaggt tgtcgttagt aggggcacaa gtggactgga
     5881 tgatgaaaat atcaccaccg cgtacatttt cgttgatttg tacactgact tcgccgtcgc
     5941 taaagcgacc tacagcggcg tcgccgagtg aagtgtacag gcggttggca atacgttgtg
     6001 ctagttccgg ggtggcgtta ccagcaaaaa gcttcatatc aggcacgaga agaacctcag
     6061 gcatgcgtcc attggtggaa agaatctgcc gaaaactgtg cgggccaggc atgatccttt
     6121 ccaggcggtg tattaaagag cgcgatgcaa cgtctggaac aaggtgacgt tgtcaccgaa
     6181 actcagcttg cccggcttaa agcatggctc tgtgcaatgg ggaaagatta acacctttcg
     6241 ccacaaagcc attgagccat tccggggctt gctctagcac ctggcgggct tcagactctg
     6301 tatcaaattc agcaaagaca caggcccctg tcccagtcag gcgcgacggg gcgtattcta
     6361 acagccagga aagcaccgca tcaacctcgc gaaaacgttt tcttgcgata acctcgcaat
     6421 cattgctgaa ttcacatttt agcaacgttt ctattgacct ttttggcgta ttgcgcggga
     6481 gttcaggatc tttaaaaatc actggtgtcg gaatacttac accagggtgc gccaccagat
     6541 accacttctc tggcggatcc accggcgtta gtatttcacc aacgccttcg gcaaacgcgg
     6601 catgcccccg aacaaagaca ggaacatctg cgcccagcgt cagccccatt tccgccagct
     6661 catccatgct tagcccgcat tgccagagat gatttaatgc caccaggacc gtcgcggcat
     6721 tggatgaacc accgccgaga ccgccgccca tcggcaaacg cttgtcaatg ctgatattcg
     6781 caccgcttcc cgtcggaaga cgcccgctgt ctgccgcagt tttcatcaac aaccgcgctg
     6841 cgcgaacgat caggttatct tcatgttcca cgccttcaac gggcgttaac agacgaatat
     6901 ccccatcgtc acgaagctca atgctgatgg tgtcgccgta atcaagaaac tgaaacagcg
     6961 tttgcagcgt gtggtaacca tccgcacgct gaccggtaat gtataaaaac agattaagtt
     7021 ttgccggaga gggccactgt gtccgcatta tttcactatc cagttatcca tttttaactt
     7081 gatgcgttga ccaccgtcgg tgagttccat attggctggc atcgcaggtt gcgttttggt
     7141 gtcataacca ccataaacaa ccttccagtt tttgccattc tggctgtagg taatttcgct
     7201 caggcggtac tggtcgtcca gtttgtagtc ggttgcatca cccggtaaac ctaaaatcca
     7261 ttggcgcaag ctgttgagcg gaattggcat tccggtcaat ttgccaatca tctcttcggc
     7321 gtcatcggcg gtataacgct gacctttatt gtcgactaac tgcacgttac ccggttgagc
     7381 attcagctcc agttccgtgc tgcccaatgg gttagtgagc agcagacggt agcgatcctg
     7441 gccggtttgc tgccagaaaa agcgggcgta cactttttgt tggtcagaaa tataagcgaa
     7501 cgcgccgcga gtctgatact gattaagatt gcgcacgtct tgctgatgct gacgccattg
     7561 tggcgaatcc gggcttttgc caggaccttt gggcgtggta acggaacagg cagtgagcac
     7621 aagagcagcc agcggtagca ggcggataag acgaaaatcg ggctggggca tagtgatgac
     7681 aagtccttga gatacgttgc agttataacc cttaatgcta gcgttaccgt ccgctatcgt
     7741 ctatgttcaa gttgtcttaa ttgccagaat ctaacggctt tcggcaatta ctccaaaagg
     7801 gggcgctctc ttttattgat cttacgcatc ctgtatgatg caagcagact aaccatatca
     7861 acgttggtat tatttcccgc agacatgacc cttttagcac tcggtatcaa ccataaaacg
     7921 gcacctgtat cgctgcgaga acgtgtatcg ttttcgccgg ataagctcga tcaggcgctt
     7981 gacagcctgc ttgcgcagcc gatggtgcag ggcggcgtgg tgctgtcgac gtgcaaccgc
     8041 acggaacttt atcttagcgt tgaagagcag gataacctgc aagaggcgtt aatccgctgg
     8101 ctttgcgatt atcacaatct taatgaagaa gatctgcgta aaagcctcta ctggcatcag
     8161 gataacgacg cggttagcca tttaatgcgt gttgccagcg gcctggattc actggttctt
     8221 ggggagccgc agatcctcgg tcaggttaaa aaagcgtttg ccgattcgca aaaaggtcat
     8281 atgaaggcca gcgaactgga acgcatgttc cagaaatctt tctctgtagc gaaacgcgtt
     8341 cgcactgaaa cagatatcgg tgccagtgct gtgtctgtcg cttttgcggc ttgtacgctg
     8401 gcgcggcaga tctttgaatc gctctctacg gtcacagtgt tgctggtagg cgcgggcgaa
     8461 accatcgagc tggtagcgcg tcatctgcgc gaacataaag tacagaagat gattatcgcc
     8521 aaccgcactc gcgaacgtgc ccaaattctg gcagatgaag ttggcgcgga agtgattgcc
     8581 ctgagtgaga tcgacgaacg tctgcgcgaa gccgatatca tcatcagttc caccgccagc
     8641 ccgttaccga ttatcgggaa aggcatggtg gagcgcgcat taaaaagtcg tcgtaaccaa
     8701 ccaatgctgt tggtggatat tgccgttccg cgcgatgttg agccggaagt tggcaaactg
     8761 gcgaatgctt atctttatag tgtggacgat ctgcaaagca tcatttcgca caacctggcg
     8821 cagcgtaaag ccgcagcggt tgaggcggaa actattgtcg ctcaggaaac cagcgaattt
     8881 atggcgtggc tgcgagcaca aagcgccagc gaaaccattc gcgagtatcg cagccaggca
     8941 gagcacgttc gcgatgagtt aaccgccaaa gcgttagcgg cccttgagca gggcggcgac
     9001 gcgcaagcca ttatgcagga tctggcatgg aaactgacta accgcttgat ccatgcgcca
     9061 acgaaatcac ttcaacaggc cgcccgtgac ggggataacg aacgcctgaa tattctgcgc
     9121 gacagcctcg ggctggagta gcagtacatc attttctttt tttacagggt gcatttacgc
     9181 ctatgaagcc ttctatcgtt gccaaactgg aagccctgca tgaacgccat gaagaagttc
     9241 aggcgttgct gggtgacgcg caaactatcg ccgaccagga acgttttcgc gcattatcac
     9301 gcgaatatgc gcagttaagt gatgtttcgc gctgttttac cgactggcaa caggttcagg
     9361 aagatatcga aaccgcacag atgatgctcg atgatcctga aatgcgtgag atggcgcagg
     9421 atgaactgcg cgaagctaaa gaaaaaagcg agcaactgga acagcaatta caggttctgt
     9481 tactgccaaa agatcctgat gacgaacgta acgccttcct cgaagtccga gccggaaccg
     9541 gcggcgacga agcggcgctg ttcgcgggcg atctgttccg tatgtacagc cgttatgccg
     9601 aagcccgccg ctggcgggta gaaatcatga gcgccagcga gggtgaacat ggtggttata
     9661 aagagatcat cgccaaaatt agcggtgatg gtgtgtatgg tcgtctgaaa tttgaatccg
     9721 gcggtcatcg cgtgcagcgt gttcctgcta cggaatcgca gggtcgtatt catacttctg
     9781 cttgtaccgt tgcggtaatg ccagaactgc ctgacgcaga actgccggac atcaacccag
     9841 cagatttacg cattgatact ttccgctcgt caggggcggg tggtcagcac gttaacacca
     9901 ccgattcggc aattcgtatt actcacttgc cgaccgggat tgttgttgaa tgtcaggacg
     9961 aacgttcaca acataaaaac aaagctaaag cactttctgt acttggtgct cgtatccacg
    10021 ctgcagaaat ggcaaaacgg caacaggccg aagcgtctac ccgtcgtaac ctgctgggga
    10081 gtggcgatcg cagcgaccgt aaccgtactt acaacttccc gcaggggcgc gttaccgatc
    10141 accgcatcaa cctgacgctc taccgtctgg atgaagtgat ggaaggtaag ctggatatgc
    10201 tgattgaacc gattatccag gaacatcagg ccgaccaact ggcggcgttg tccgagcagg
    10261 aataatggaa tatcaatact ggttacgtga agcaataagc caacttcagg cgagcgaaag
    10321 cccacgccgt gacgctgaaa tcttattgga gtatgtcact ggcagggggc ggacttttat
    10381 tctggccttt ggtgaaacgc agctgactga cgaacaatgt cagcaacttg atgcgctact
    10441 gacacgtcgt cgcgatggtg aacccattgc tcatttaacc ggggtgcgag aattctggtc
    10501 gttgccgtta tttgtttcgc cagcgacctt aattccgcgc ccggatacgg agtgtctggt
    10561 ggagcaggca ctggcgcggt tgcctgaaca accttgccgt attctcgatc tcgggacggg
    10621 taccggggcg attgcgcttg cgctggctag cgagcgcccg gactgcgaaa ttatcgctgt
    10681 agatcgtatg cctgatgctg tctccctggc acaacgtaat gcccagcatc tggcgatcaa
    10741 aaatatccac attctgcaaa gcgactggtt tagcgcgcta gccgggcagc agtttgcgat
    10801 gattgtcagc aatccgccgt atattgacga gcaggaccca catcttcaac aaggcgatgt
    10861 ccgctttgag ccgctcactg cgctggttgc ggcagacagt ggaatggcag acatcgtgca
    10921 tatcatcgaa cagtcgcgta acgcgctggt atccggcggc tttctgcttc tggaacatgg
    10981 ctggcagcag ggcgaagcgg tgcgacaagc atttatcctc gcggggtatc atgacgtcga
    11041 aacctgccgt gactatggtg ataacgagcg cgtaacgctc ggccgctatt atcaatgaca
    11101 agtttttcta cactgcttag tgttcatctt attagtatcg cgctttctgt tgggctatta
    11161 accttacgtt tctggctacg ttatcagaag catccacagg catttgctcg ctggacgcgc
    11221 attgtgccgc cggttgtcga tacggtgtta ttgttaagcg gcattgcgtt gatggctaaa
    11281 gcgcacatcc tgccattttc cgggcaggca cagtggctga ctgaaaagct gtttggagtt
    11341 atcatttata tcgttttggg ttttattgca ctcgattatc gtcgtatgca cagtcagcag
    11401 gcgcgcatta ttgccttccc gctggcgttg gtggtgctgt acatcatcat taaactcgcc
    11461 accacaaaag taccgttact ggggtaagtc atgagatcgt tagctgattt cgaatttaat
    11521 aaagcgccat tgtgcgaagg catgatcctg gcttgcgaag caatccgccg cgattttccc
    11581 tcgcaagatg tttacgacga actggagcgt ctcgttagtc tggcgaagga agaaatcagc
    11641 cagcttctgc ctttagaaga gcagctggaa aaactgatcg cgctgtttta cggcgactgg
    11701 ggatttaaag cctcacgcgg cgtttatcgt ctttccgatg cattatggct ggaccaggtg
    11761 ttaaagaatc gacaggcagt gcggtatcat taggtgcggt tttattatgg gtcgcgaatc
    11821 gtctcgattt gccgctgctg ccggtgattt tccctacgca gctgatattg cgcattgaat
    11881 gtccggatgg cgaaatttgg ctgattaatc cttttaacgg tgaatcgtta agcgaacata
    11941 tgctggacgt atggttaaag ggaaatatca gcccgtcggc ggaactgttt tatgaagacc
    12001 ttgatgaagc tgataacatt gaggtaatcc gcaaattgtt ggatacactc aaagcctcgt
    12061 tgatggaaga aaatcagatg gagctggcgt tacgcaccag cgaagcttta ttacaattca
    12121 acccggaaga tccctatgaa attcgcgatc gcgggttgat ttatgcgcaa ctggattgcg
    12181 aacacgttgc gttgaacgat ttaagttatt tcgttgaaca gtgtccggaa gacccgatca
    12241 gcgaaatgat ccgtgcgcaa ataaataaca tcgcgcataa acatattgtg ctgcattaat
    12301 taatcgacat tttactcaag attaaggcga tcctatgaaa caaaaagtgg ttagcattgg
    12361 cgacatcaac gtagcaaatg acctgccgtt cgtactgttt ggtggtatga acgtgctgga
    12421 atctcgcgat ctggcgatgc gcatttgcga gcactacgta accgtgaccc agaaactggg
    12481 tatcccttac gtgttcaaag cctcttttga caaagccaac cgctcctcca tccactctta
    12541 tcgtggaccg ggcctggaag aagggatgaa aatcttccag gagttgaagc agacttttgg
    12601 cgtgaaaatt atcaccgacg ttcacgaacc aagtcaggca cagcccgttg ctgatgtcgt
    12661 ggatgtgatt cagttgccgg cgtttcttgc tcgccagact gacctggttg aagccatggc
    12721 gaaaaccggt gcggtaatta acgtcaagaa accacagttt gtcagcccgg gacagatggg
    12781 taatatcgtt gataaattca aagaaggcgg caacgaaaaa gtgattcttt gcgatcgcgg
    12841 tgctaacttc ggctatgaca acctggttgt cgatatgctg ggcttcagca ttatgaagaa
    12901 agtgtctggt aactcgccgg tgattttcga cgtgacccac gcactgcaat gccgcgatcc
    12961 gtttggcgca gcttccggtg gtcgtcgtgc tcaggtggct gagctggcac gagccggtat
    13021 ggcggtaggt ctggcggggc tgtttattga agcgcatccg gatccggaac atgcgaaatg
    13081 tgatggtcca tccgcgctgc cgctggctaa actggaaccg ttcctcaagc agatgaaagc
    13141 gattgatgat ctggtgaaag gtttcgaaga actggatacc agcaagtaat cttttttgct
    13201 tgaaaaataa agccttagcg ttctgcgtta agactttttt catgggtgcc agatacaaaa
    13261 a
//
LOCUS       DAJTZL010000117        13158 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.117, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000117 DAJTZL010000000
VERSION     DAJTZL010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13158
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.117"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            50..2140
                     /locus_tag="OO544_002876"
     CDS             50..2140
                     /locus_tag="OO544_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCR6768418.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQLLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDSPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(2207..3427)
                     /gene="argD/dapC"
                     /locus_tag="OO544_002877"
     CDS             complement(2207..3427)
                     /gene="argD/dapC"
                     /locus_tag="OO544_002877"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HCR6768419.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGEILWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINPPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQYKGRARDFLYAGAEAGVMVLNAGPDVMRFAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(3513..4076)
                     /gene="pabA"
                     /locus_tag="OO544_002878"
     CDS             complement(3513..4076)
                     /gene="pabA"
                     /locus_tag="OO544_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HCR6768420.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHSGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHR"
     gene            complement(4108..4710)
                     /locus_tag="OO544_002879"
     CDS             complement(4108..4710)
                     /locus_tag="OO544_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HCR6768421.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSKAGESE"
     gene            complement(4700..4867)
                     /locus_tag="OO544_002880"
     CDS             complement(4700..4867)
                     /locus_tag="OO544_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HCR6768422.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(4972..5544)
                     /gene="ppiA"
                     /locus_tag="OO544_002881"
                     /gene_synonym="rotA"
     CDS             complement(4972..5544)
                     /gene="ppiA"
                     /locus_tag="OO544_002881"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HCR6768423.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            5815..6996
                     /gene="tsgA"
                     /locus_tag="OO544_002882"
     CDS             5815..6996
                     /gene="tsgA"
                     /locus_tag="OO544_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HCR6768424.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSTA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            7258..9801
                     /gene="nirB"
                     /locus_tag="OO544_002883"
     CDS             7258..9801
                     /gene="nirB"
                     /locus_tag="OO544_002883"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HCR6768425.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            9798..10124
                     /gene="nirD"
                     /locus_tag="OO544_002884"
     CDS             9798..10124
                     /gene="nirD"
                     /locus_tag="OO544_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HCR6768426.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            10250..11056
                     /gene="nirC"
                     /locus_tag="OO544_002885"
     CDS             10250..11056
                     /gene="nirC"
                     /locus_tag="OO544_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HCR6768427.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHAMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            11075..12448
                     /gene="cobA"
                     /locus_tag="OO544_002886"
     CDS             11075..12448
                     /gene="cobA"
                     /locus_tag="OO544_002886"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCR6768428.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            12695..12862
                     /locus_tag="OO544_002887"
     CDS             12695..12862
                     /locus_tag="OO544_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HCR6768429.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
BASE COUNT         3107 a         3461 c         3357 g         3233 t
ORIGIN      
        1 cccgtcggag tggcgcgtta cctggtagcg cgccattttg tttcccccga tgtggcgcag
       61 actgatttat caccccgata tcaactatgc acttcgacaa acgctggtgc tatgtttgcc
      121 cgtggccgtt gggttaatgc ttggcgaatt acgattcggt ctgctcttct ccctcgttcc
      181 tgcctgttgc aatattgcgg gccttgatac gcctcataaa cgttttttca aacgcttaat
      241 cattggtgcg tcgctgtttg ccacctgtag cttgctgaca cagctactac tggcaaaaga
      301 tgttcccctg ccctttttgc tgaccggatt aacgctggta cttggcgtca ctgctgagct
      361 ggggccattg cacgcaaaat tgcttcctgc atcgctgctc gccgccattt ttaccctcag
      421 tctggcggga tacatgccgg tctgggaacc gttgctcatc tatgcgttgg gcactctctg
      481 gtacggattg tttaactggt tttggttctg gatctggcgc gaacaaccgc tgcgcgagtc
      541 actaagtctg ctgtaccgcg aactggcaga ttattgtgaa gccaaataca gcctgcttac
      601 ccagcacacc gaccctgaaa aagcgctgcc gccgctgctg gtgcgccagc aaaaagcggt
      661 cgatctaatt acccagtgct atcagcaaat gcatatgctt tccgcgcaaa ataatactga
      721 ttacaagcgg atgctgcgta ttttccagga ggcgctggat ttacaggaac atatttcggt
      781 cagtttgcat cagccggaag aggtgcaaaa gctggtcgag cgtagccatg cggaagaagt
      841 tatccgctgg aatgcgcaaa ccgtcgccgc tcgcctgcgc gtgctggctg atgacattct
      901 ttaccatcgc ctgccaacgc gttttacgat ggaaaagcaa attggcgcac tggaaaaaat
      961 cgcccgccag catccggata atccggttgg gcaattctgc tactggcatt tcagccgcat
     1021 cgcccgcgtg ctgcgcaccc aaaaaccgct ctatgcccgt gacttactgg ccgataaaca
     1081 gcggcgaatg ccgttacttc cggcgctgaa aagttatctg tcactaaagt ctccggcgct
     1141 acgcaatgcc ggacgactca gtgtgatgtt aagcgttgcc agcctgatgg gcaccgcgct
     1201 gcatctgccg aagtcgtact ggatcctgat gacggtattg ctggtgacac aaaatggcta
     1261 tggcgcaacc cgtctgagga ttgtgaatcg ctccgtggga accgtggtcg ggttaatcat
     1321 tgcgggcgtg gcgctgcact ttaaaattcc cgaaggttac accctgacgt tgatgctgat
     1381 taccaccctc gccagctacc tgatattgcg caaaaactac ggctgggcga cggtcggttt
     1441 tactattacc gcagtgtata ccctgcaact attgtggttg aacggcgagc aatacatcct
     1501 tccgcgtctt atcgatacca ttattggttg tttaattgct ttcggcggta ctgtctggct
     1561 gtggccgcag tggcagagcg ggttattgcg taaaaacgcc catgacgctt tagaagccta
     1621 tcaggaagcg attcgcttga ttcttagcga ggatccgcaa cctacgccac tggcctggca
     1681 gcgaatgcgg gtaaatcagg cacataacac tctgtataac tcattgaatc aggcgatgca
     1741 ggaaccggcg tttaacagcc attatctggc agatatgaaa ctgtgggtaa cgcacagcca
     1801 gtttattgtt gagcatatta atgccatgac cacgctggcg cgggaacacc gggcattgcc
     1861 acctgaactg gcacaagagt atttacagtc ttgtgaaatc gccattcagc gttgtcagca
     1921 gcgactggaa tatgacgaac cggggagttc tggcgatgcc aatatcatgg attcgccgga
     1981 gatgcagccg cacgaaggcg cggcaggtac gctggagcag catttacagc gggttattgg
     2041 tcatctgaac accatgcaca ccatttcgtc gatggcatgg cgtcagcgac cgcatcacgg
     2101 gatttggctg agtcgcaagt tgcgggattc gaaggcgtaa tgcaggctaa accatcggat
     2161 aaggcattca cgccgcatcc gacatctttt gcctgatgct tcgtatttac gccccaacca
     2221 ccttcgccac cgcgtgggcg aaacgttgca tcccttcatc gatatccgca tcttccacca
     2281 ccagcgacgg tgcaaaacgc atcacatccg gtccggcatt cagcaccatt acgccagcct
     2341 ctgcgcccgc atacaggaaa tcacgcgccc gacctttgta ctgtggtttc agctctgcgc
     2401 caatcaacag ccccataccg cgaatatcgc taaacacatc gtactgctga tcgatcttct
     2461 gcagatggtc aacaaaacgc tggcgtttcg cctgaatgcc ttccagcact tcagggggat
     2521 tgatgatatc aaacgccgcc cccgctactg cacaggccag aggattaccg ccgtaggtgg
     2581 aaccgtgaga accaggatga aacgcagaag caatttccgc cgtggtcagc atggcgctaa
     2641 tcgggaagcc gccgcctaac gctttcgcag aggtcagaat atccggcgtc acgccgtagt
     2701 gcatgtaagc aaacaaatcg ccggtccgcc ccatcccgca ctgcacttca tcaaacacca
     2761 ataatgcctg atgttgatcg cacagctcgc gcaagccctg caaaaactct ggcgtcgcag
     2821 ccgtcacgcc gccctcgccc tggatcggct caaccaccac cgcacaggtg tgatcatcca
     2881 tcactgcttt cactgcgtgg agatcgttaa agggaacgtg gatgatgtct gccggtttcg
     2941 gcccaaagcc gtcggaatat tttggctgcc cacccaccga aacggtaaac agcgagcgac
     3001 catgaaaagc gttatggaag gcaataattt tggttttgaa cgggctatga cgcacacagg
     3061 cgtaatgacg tgccagttta aaggcggttt cgttagcttc cgtgccggag ttcataaaca
     3121 ccacgcgttc ggcaaacgtt gcctcaatca gtttacgccc aagacgcagc gccggttcat
     3181 tggtgaaaac gttactgata tgccacagaa tttcgccctg ggtttttaac gcgttcacca
     3241 gcgcaggatg gcaatggccc aacgccgtaa ctgcaatgcc acccgcgaaa tcgacatact
     3301 ccttgccttg ctgatcccag attcggctgc cctgaccttt taccggaata aactctgccg
     3361 gagcataaat cggcaggatc acttcatcga aagtcgcgcg tgtaattgct gtttgttcaa
     3421 ttgccatctc atgatcaccc tgttacgcat aaacaaatgt gaaattataa tcacaaaata
     3481 tgcataaaaa atcactaaat ggcaatcaga aatcagcgat gcaggaaatt agccagcagt
     3541 tgatgtcctt gttcgctaag aatactttct ggatggaact gcacaccttc cagatcccac
     3601 tggcgatggc gaatccccat aatctcgcgg gtttcgctcc aggccgtcac gtcaaagcac
     3661 gctggtaatg agtcaggttc caccaccagc gaatggtagc gtgtcacggt aagtggattt
     3721 gccagccccc gaaatacgcc ctcaccgcta tgtgtaatcg gcgaggtttt gccgtgcatg
     3781 acctttgcgg cgcgcacaac tttgccgcca aatgcctgcg ccattgcctg atgaccgagg
     3841 cagacgccaa gaatcggcaa gcgcccggca tagtgacgaa taacgtcaag ggagatcccg
     3901 gcttcatctg gcgtacaggg gccaggtgag atgacaattt tttgtggttt aagggcgtcg
     3961 atatccgcca gcgtcaacgc atcgttgcgc ttaaccagca catccgcccc cagttcacaa
     4021 aagtactggt agaggttcca ggtaaaagaa tcgtagttat ctataagcag gatcatggcg
     4081 gctccggtac gaaagaaccg cgctatttta ctcagattcc ccggctttgc ttaccacttt
     4141 gctaaatatc atctgcagtg cggtgagatc ccccattgcc ccactctgat ttgcctgatt
     4201 ccaggcctct ttttcgatac cctgccagct cagttgatat ccggcatgaa tcgccagttg
     4261 ctcgaagaag atccgctgtg ccagaccact tcccacccgg aagggatgca gcacgttgat
     4321 ttcacaatag taatgcgcca gccgctcgac gaacttcgct ttctccaggc caaccagata
     4381 accttcttcc tccagatcct gcatcagggc attgccctct ttttcgatat aagcaaagtg
     4441 gcagaacggc gtatcgccct gataaatatc aacttcacgc agttgccctg cccagtcgaa
     4501 aatatcctgg tacagctggc gatggatagt gcgcaaatgc ggtaaaccgc gcaccagcgg
     4561 accaagctca atggtcgcag cacgcagcgc cgtcatttcg taagcggcct gttccagccg
     4621 ctgctgctga cggatgttca gccggttacg catgatatca aggccaggat aaagatacgg
     4681 atcgcgccct tcaccgaatt tatcgctcat agtgcctcct cagctcttca aggcgcgcta
     4741 aagcctctgc ggcagtaaga gtgactaaag gcatctcgac gccttcaaga cggcgactgg
     4801 cctggaaatt acgattacgc tgaagctccc agagacggga cttttgctta tcggtgagtt
     4861 tcttcacttg atgcctccct gaacgtgtcc atttgccaca agtataagcg gcaaatccgg
     4921 gttacgccgg gagagcagaa gcgcgggcaa gattgcccgc gcgagaaatc attacggcag
     4981 gactttagcg gaaaggataa ctaccggttt tgacggcaca ttctggtacg gaccaacatc
     5041 atgagtcggc acctgggaaa tcttatcggc aacgtccatg cctttcacca ctttaccaaa
     5101 taccgcgtaa ccgaaatcac gctgaccatg gtcaaggaag gcgttatcgg caacgttgat
     5161 aaagaactgg ctggtggcgc tgtctttgtc agcggtacgc gccatcgcga tggtgccacg
     5221 cgtgttgcgc aggccgttat cggcttcatt tttgattggc gggtttggtt ttttctgctg
     5281 catctgctcg gtgaaaccgc cgccctgaat cataaagcca ggaatgacgc ggtgaaaggt
     5341 agtgttgtta taaaaaccgc tgttcacata atcgacaaag ttttgcacag acactggcgc
     5401 tttttgttta tccagctcca gttcgatgtt accagctgag gttgtcaaca atacgtgcgg
     5461 gtcccctttc gctgccattg ctgcgggaga aagagcagaa agagcgaaaa cagcagccat
     5521 cgccgccagg gtcgatttga acatgagatt tccttaacaa acagagcaga aaaaaagcaa
     5581 gtgaagagat tctaaatagc cttcaagaac aaggccatcc ctttacctaa ttttacgtat
     5641 ctgaaatata tgtaacctga cgatcacttt ttattccgtg atcaaaatca cctcttaaaa
     5701 tgcaatttag caaccgattg caataaaaca tttaaacaga tcacaaaatc acctaaaatc
     5761 gcccgctcgc ggcatatccc gctatgccat tttatttttg aacgcaggcc aatcatgact
     5821 aacagcaatc gcatcaagct cacatggatt agctttctct cctacgcact gactggtgcg
     5881 ttggttattg tcaccgggat ggtgatggga aatatcgccg attatttcaa tctgcctgtt
     5941 tccagtatga gtaacacctt caccttcctc aacgccggca ttttaatctc tatcttcctc
     6001 aacgcctggc tgatggaaat cgtcccgttg aaaacgcagt tacgttttgg ctttctcctg
     6061 atggtgctgg cggttgccgg tttgatgttc agccacagcc tggcgctgtt ctcgacggcg
     6121 atgttcattc tcggggtggt cagcggcatt actatgtcga ttggtacatt cctgataaca
     6181 caaatgtatg aagggcgtca gcgcggttcc cgcctgttat ttactgactc cttcttcagt
     6241 atggctggga tgattttccc aatgatcgcc gcgtttctac tggcgcgcag cattgagtgg
     6301 tactgggttt atgcctgcat cgggctggtg tatgtcgcta tttttattct gaccttcggc
     6361 tgtgagttcc cggcactggg caaacatgca ccaaaaacgg atgctccggt agagaaagaa
     6421 aagtggggga tcggcgtact gtttctctct gttgcggcgc tgtgctacat cctcggtcag
     6481 ttaggtttta tctcctgggt gcctgagtat gccaaaggcc tgggcatgag cctgaacgac
     6541 gcgggcacgc tggtgagtaa cttctggatg tcatacatgg tcggcatgtg ggcgttcagc
     6601 tttattcttc gcttctttga tttgcaacgc attctgaccg tactggctgg tctggctgcg
     6661 attctgatgt acgtctttaa caccggaaca ccagcacata tggcgtggtc aattctcgct
     6721 ctgggcttct tctccagcgc gatctatacc accatcatca ccctgggttc acagcagacc
     6781 aaagtaccgt cgccaaaact ggttaacttt gtcctgacct gcggaaccat cggtactatg
     6841 ttgacctttg tggttaccgg cccgattgtt gaacatagcg gtccgcaggc ggcactgctt
     6901 acagcaaacg gtctgtacgc tgtcgtcttt gtgatgtgct tcctgttagg tttcgtcagc
     6961 cgtcaccgtc agcataacac cctgacctct cattaattgc taatgccgga cggcactatc
     7021 gtcgtccggc cttttcctct cttcccccgc tacgtgcatc tatttctata aacccgttca
     7081 ttttgtccat tttttgcaca aacatgaaat gtcagacaat tccgtgactt aagaaaattt
     7141 atacaaatca gcaacatacc acttaaggag tatataaagg tgaatttgat ttacatcaat
     7201 aagcggggtt gctgaatcgt taaggtaggc ggtaatagaa aagaaatcga ggcaaaaatg
     7261 agcaaagtca gactcgcaat tatcggtaac ggtatggtcg gccatcgctt tatcgaagat
     7321 cttcttgata aatctgatgc ggccaacttt gatattaccg ttttctgtga agaaccgcgc
     7381 atcgcttatg accgcgtaca cctctcgtct tacttctctc accacaccgc cgaagagctg
     7441 tcgctggtgc gcgaaggctt ctacgagaaa cacggcatca aagttctggt cggcgaacgc
     7501 gctatcacca tcaaccgtca ggagaaggtg attcactcca gcgccggacg taccgttttt
     7561 tatgacaagc tgatcatggc aaccggttcc tacccgtgga tcccaccaat caaaggttct
     7621 gatactcagg actgctttgt ctatcgcacc attgaagacc tcaacgccat tgaatcctgc
     7681 gcccgtcgca gcaaacgcgg tgccgttgtt ggtggcggcc tgttaggtct ggaagccgct
     7741 ggcgcgctga aaaacttagg tattgaaacc cacgttatcg aatttgcccc tatgctgatg
     7801 gcggaacagc ttgatcagat gggtggcgag cagctgcgtc gcaaaatcga aagtatgggc
     7861 gtgcgcgttc acaccagcaa aaacaccctt gagattgtgc aggaaggtgt cgaagcgcgt
     7921 aaaaccatgc gttttgccga cggcagcgaa ctggaagtcg actttatcgt cttctctacc
     7981 ggtatccgtc cgcgcgataa gctggcaacc cagtgtggtc tggacgttgc tccgcgtggg
     8041 ggtatcgtca ttaatgattc ctgccagact tccgatccgg atatctacgc catcggtgaa
     8101 tgcgcaagct ggaacaaccg tgtatttggt ctggtagcac ctggctacaa aatggcgcag
     8161 gtcgccgttg accatattct cggtagcgaa aacgcctttg aaggtgctga ccttagcgcc
     8221 aagctgaaac tgctgggcgt agacgtaggc ggtattggtg atgcgcacgg tcgcacgcct
     8281 ggcgcacgta gctacgttta cctcgacgaa agtaaagaga tctacaaacg cctgattgtc
     8341 agcgaagaca acaaaaccct gctcggtgcg gtactggtgg gcgataccag cgactacggt
     8401 aacctgctgc aactggtgct gaacgctatc gaactgccgg aaaacccgga ttccctgatc
     8461 ctgccagcac actctggtag cggcaagccg tctatcggtg ttgataaact gccggacagc
     8521 gcgcaaatct gctcctgctt cgacgtcact aaaggtgatc tgattgctgc catcaacaaa
     8581 ggctgccaca cagttgcggc gctgaaagct gaaaccaaag cgggtactgg ctgcggtggc
     8641 tgtatcccgc tggtcactca ggtactgaac gcggaactgg cgaaacaggg catcgaagtt
     8701 aacaacaacc tgtgcgaaca ctttgcttat tcgcgtcagg aactgttcca tttgatccgc
     8761 gttgaaggca ttaaaacctt cgaagaactg ctggcgaaac acggcaaagg ctacggttgt
     8821 gaagtttgta aaccaaccgt cggttcgctg ctggcctcct gctggaacga atacattctg
     8881 aagccggaac atactccgct gcaggattct aacgacaact tcctcgctaa catccagaaa
     8941 gacggcacct actcggtgat cccgcgttct ccgggcggtg aaatcacccc ggaagggctg
     9001 atggcagtgg gtcgtatcgc gcgtgaattt aatctctaca ccaaaatcac cggctcccag
     9061 cgcctggcga tgtttggcgc acagaaagac gatctgccgg aaatctggcg tcagctgatt
     9121 gaagctggct tcgaaaccgg tcatgcctat gcgaaagcac tgcgtatggc gaaaacctgc
     9181 gtgggtagca cctggtgccg ctacggtgtt ggcgatagcg tcggcctcgg cgtggaactg
     9241 gaaaaccgct acaaaggcat ccgtacgccg cacaaaatga agttcggtgt ctccggctgt
     9301 acccgtgaat gttcagaagc tcagggtaaa gacgtgggta ttatcgccac tgaaaaaggc
     9361 tggaacctgt atgtttgcgg taacggcggc atgaaaccgc gtcatgcgga tctgctggca
     9421 gcggatatcg atcgcgaaac gctgatcaaa tatctcgacc gcttcatgat gttctacatc
     9481 cgtaccgccg acaaactgac gcgtaccgcg ccgtggttag aaaacctcga aggcggcatc
     9541 gattacctga aagcggtgat cattgacgac aaactggggc tgaacgctca tctggaagaa
     9601 gagatggcgc gcctgcgtga agcggtagtg tgtgagtgga ctgaaacggt caatacgccg
     9661 tctgcgcaga ctcgcttcaa acacttcatc aacagcgaca agcgtgaccc gaacgtgcag
     9721 atggtgccag agcgcgaaca gcaccgtccg gcaacgccgt atgaacgtat cccggtaact
     9781 ctggtggagg acaacgcatg agccagtgga aagacatctg caaaatcgat gacatcctgc
     9841 ctgaaaccgg cgtctgcgcg ctgttaggtg acgagcaggt agcgattttc cgcccgtatc
     9901 acagcgacca ggtgtttgcg atcagcaaca ttgatccgtt cttcgagtcc agcgtgctat
     9961 cacgcgggct gattgcggaa caccagggcg agctgtgggt cgccagcccg ctgaaaaaac
    10021 agcgttttcg cttaagtgat ggcttgtgca tggaagacga acagttttcc gtcaaacatt
    10081 acgaagcgcg agtgaaagac ggcgtggtgc agctgcgcgg ttaatgtttt aacgggaggc
    10141 gcaatgcctc ccctttttgc atggtcctgt aatgaccttc ggtatattgc agaacatttt
    10201 ttaaactttt tgttttattt tttgttttta ttttttaaag gataatcaaa tgtttacaga
    10261 cactattaat aagtgtgcgg ctaatgctgc gcgcattgca cgcctgtcgg caaataaccc
    10321 gctcggcttt tgggtcagct ccgccatggc gggcgcatat gtgggtcttg ggatcatcct
    10381 gattttcacg ctcggtaatt tgctcgaccc gtccgtacgt ccgctggtga tgggcgcgac
    10441 ctttggtatc gccttaacgc tggtgattat cgccggttct gaactgttca ccggacacgc
    10501 catgttcctc acctttgggg taaaagcggg cagcatcagc cacgggcaaa tgtgggcaat
    10561 cctgccgcaa acctggctgg gtaacctggt cggttccgtc ttcgttgcca tgctctatag
    10621 ctggggcggc ggtagcctgc tgccggtaga taccagcatc gttcactccg tcgcgctggc
    10681 taaaaccact gcaccggcaa tggtactgtt cttcaaaggt gcattgtgta actggctggt
    10741 ttgcctggct atctggatgg cgctgcgcac tgaaggggcg gcgaaattta tcgctatctg
    10801 gtggtgtctg ctggcattta tcgcgtccgg ctacgagcac tccatcgcta acatgacgct
    10861 gttcgccctc tcctggttcg gcaaccacag cgaagcctac acgctggcgg gtattggtca
    10921 taacctgctg tgggtgacgc tgggtaatac tttatcgggt gccgtattca tgggattggg
    10981 ttattggtat gctacgccga aagcgaatcg tccggttgcg gacaaattta atcaaactga
    11041 aacggctgcc ggttaattac taaggggttt ttacgtggat catttgccta tattttgcca
    11101 attacgcgat cgcgactgtc tgattgtcgg cggtggtgat gtcgcggaac gcaaagcaag
    11161 gttgctgtta gacgcaggcg ctcgcttaac ggtgaatgca ttagcgttta ttccacagtt
    11221 caccgcatgg gcagatgcag gcatgttaac cctcgtcgaa gggccatttg atgaaagcct
    11281 tctcgacacc tgctggctgg cgattgcagc gacggatgat gacacgctta accagcgcgt
    11341 cagcgaagcc gctgaagctc gtcgcatctt ctgtaacgta gtcgatgcgc cgaaagccgc
    11401 cagctttatt atgccgtcga ttattgaccg ctcaccgcta atggtagcgg tctcctctgg
    11461 cggcacctct ccggttctgg cgcgcctgtt gcgcgaaaaa ctcgaatcac tgctgccgtt
    11521 gcatctgggc caggtggcga aatatgccgg gcaattacgc gggcgagtga aacaacagtt
    11581 cgccacgatg agtgagcgtc gccgtttctg ggagaaattg ttcgttaacg accgcctggc
    11641 gcagtcgctg gcaaacaacg atcagaaagc cattactgaa acgaccgaac agttaatcaa
    11701 cgaaccgctc gaccatcgcg gtgaagtggt gctggttggt gcaggtccgg gcgatgccgg
    11761 gctgctgacg ctgaaaggac tgcaacaaat tcagcaggca gatgtggtgg tctacgaccg
    11821 tctggtttct gacgatatta tgaatctggt acgccgcgat gctgatcgcg ttttcgtcgg
    11881 caaacgcgcg ggataccact gtgtaccgca ggaagagatt aaccagatcc tgctgcggga
    11941 agcgcaaaaa ggcaaacgcg tggtgcggct gaaaggtggc gatccgttta tttttggccg
    12001 tggtggcgaa gagctggaaa cactgtgcaa cgcgggtatt ccgttctcgg tggttccggg
    12061 tattaccgca gcttctggtt gctctgccta ttcgggtatt ccactcacgc atcgcgatta
    12121 tgcccagagc gtacgcttaa ttaccggaca cttaaaaacc ggtggcgagc tggactggga
    12181 aaacctggcg gcagaaaaac agacgctggt gttctatatg ggattgaatc aggccgcgac
    12241 tattcagcaa aagctgattg aacacggtat gcctggcgaa atgccggtgg caattgtcga
    12301 aaacggaacg gcagtcacgc agcgcgtgat tgacggtacg ctcacgcaac tcggtgaact
    12361 tgctcagcaa atgaacagtc catcgctaat tattattggt cgggttgttg gcctgcgcga
    12421 taaactgaac tggttctcca accattaatt taacccgggc cagagaattc tggccttctt
    12481 aatgactctt tttatatatt cagcaaataa aacataaccc cttaataaat attatctgct
    12541 aatgggttat attcaccgtt acagcgactt acaaaattaa gtcattcgct cactgacgcc
    12601 aattaaatat tcctgtatcg ttcttaacga gcagtctgct tatattcata taatcaatga
    12661 atattaatta atatataata catagggaat gtaaatgaac aaatttatta aagttgcact
    12721 ggtaggtgca gtactggcta cgttaactgc atgtactggt catattgaaa accgtgataa
    12781 gaactgctct tacgactacc tgctgcaccc ggcaatttct atttctaaaa tcattggcgg
    12841 ttgcggtcct actgcacagt aagtctctga cagaaatcgg ctaacaccgg tttaattgcc
    12901 cgacgccctc tggattttcc gagggcgtat ttgtttctat gtccttcttg cccccgcccg
    12961 taaaaatatt ttcactttaa attcaatttg ataactacat ccattagcca cagcgcgatc
    13021 ctgtgcgaaa ttttgtgatc ttcctccaca ttacataaca tcatatgttg ttatattcat
    13081 catgcattgt catgttacct tttaaatgac tgcaaactct cccctacaac gtattggaca
    13141 agaaaaaggt atcgctat
//
LOCUS       DAJTZL010000118        13040 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.118, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000118 DAJTZL010000000
VERSION     DAJTZL010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13040
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.118"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            753..1181
                     /gene="uspC"
                     /locus_tag="OO544_002888"
     CDS             753..1181
                     /gene="uspC"
                     /locus_tag="OO544_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HCR6768430.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKFIQDAGYPVDKTFIAYGELSEHILEVCHKHH
                     FDLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
     gene            complement(1188..2612)
                     /gene="otsA"
                     /locus_tag="OO544_002889"
     CDS             complement(1188..2612)
                     /gene="otsA"
                     /locus_tag="OO544_002889"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HCR6768431.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNQFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTAWGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFPKLA"
     gene            complement(2587..3387)
                     /gene="otsB"
                     /locus_tag="OO544_002890"
     CDS             complement(2587..3387)
                     /gene="otsB"
                     /locus_tag="OO544_002890"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HCR6768432.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            complement(3554..4540)
                     /gene="araH"
                     /locus_tag="OO544_002891"
     CDS             complement(3554..4540)
                     /gene="araH"
                     /locus_tag="OO544_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HCR6768433.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGILCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRIV"
     gene            complement(4555..6069)
                     /gene="araG"
                     /locus_tag="OO544_002892"
     CDS             complement(4555..6069)
                     /gene="araG"
                     /locus_tag="OO544_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP binding protein
                     AraG"
                     /protein_id="HCR6768434.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADQHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRSIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            complement(6139..7128)
                     /gene="araF"
                     /locus_tag="OO544_002893"
     CDS             complement(6139..7128)
                     /gene="araF"
                     /locus_tag="OO544_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HCR6768435.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITANELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDALSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKDVEPPKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            7925..8428
                     /locus_tag="OO544_002894"
     CDS             7925..8428
                     /locus_tag="OO544_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HCR6768436.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(8507..8758)
                     /locus_tag="OO544_002895"
     CDS             complement(8507..8758)
                     /locus_tag="OO544_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 domain-containing protein"
                     /protein_id="HCR6768437.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTNHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(8873..8959)
                     /gene="azuC"
                     /locus_tag="OO544_002896"
     CDS             complement(8873..8959)
                     /gene="azuC"
                     /locus_tag="OO544_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HCR6768438.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            9222..9545
                     /locus_tag="OO544_002897"
     CDS             9222..9545
                     /locus_tag="OO544_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HCR6768439.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAVPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            9716..10213
                     /gene="ftnA"
                     /locus_tag="OO544_002898"
     CDS             9716..10213
                     /gene="ftnA"
                     /locus_tag="OO544_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HCR6768440.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(10251..10490)
                     /locus_tag="OO544_002899"
     CDS             complement(10251..10490)
                     /locus_tag="OO544_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family protein"
                     /protein_id="HCR6768441.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSKDGFSTDQSKICRH"
     gene            10681..11892
                     /gene="tyrP"
                     /locus_tag="OO544_002900"
     CDS             10681..11892
                     /gene="tyrP"
                     /locus_tag="OO544_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HCR6768442.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGV
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNVRKLRWVF
                     ITGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(11954..12619)
                     /gene="yecA"
                     /locus_tag="OO544_002901"
     CDS             complement(11954..12619)
                     /gene="yecA"
                     /locus_tag="OO544_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HCR6768443.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTNHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(12815..12901)
                     /locus_tag="OO544_002902"
     tRNA            complement(12815..12901)
                     /locus_tag="OO544_002902"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12865..12867),aa:Leu,seq:taa)
     gene            complement(12914..12987)
                     /locus_tag="OO544_002903"
     tRNA            complement(12914..12987)
                     /locus_tag="OO544_002903"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12953..12955),aa:Cys,seq:gca)
BASE COUNT         3342 a         3243 c         2968 g         3487 t
ORIGIN      
        1 tttttatgcg gtttcaccgc accccgtgat gtcgccggga agccccggta aaaaataatt
       61 agcattagaa tagttgcgat aagctgcaat aagcagaacc acctttttgg tttaatatgt
      121 ccttacaaat agaaatgggt ctttacactt atctaagatt tttcctaaat cgacgcaact
      181 gtactcgtca ctacacgcac atacaacgga ggggggctgc gattttcaat aatgtgtgat
      241 gcagatcaca caaaacactc aattacttaa cataaatgta taagtcatac ttttgttttg
      301 ggtgtatttc aatatgttaa aaagtttttc gctacgctag caagcaaaaa tgaaacagga
      361 ataatcgaaa tgggatgttg cgcacagtta aaataaatca ccgtaaataa tcatctgcta
      421 taaataatca ctttcatgca ataccagata agctattttt aaacagacac ttaccacaca
      481 acaaactaat taaatacaaa gaaaggcaac aatcaactta atgttaatga aatgttttac
      541 atacattaac cacttgttta atgcggagaa tcagtacact attttcttat aattacattt
      601 gaaattatat gatcaccggg agtgattgat taattgtctt tactaataat cagactaata
      661 tttacctgtt tgaccgagtt gggattgcgt cgtttctcca ttaggagtaa agctttaatg
      721 tcacctgaag ttcacagaat aaggaacagg ttatgagcta tagcaatatt cttgtcgctg
      781 ttgcggttac accggaaagt cagcaactgc tggcaaaagc ggtatctatc gccaggccag
      841 taaagggaca catcagttta attactctcg cttccgaccc ggaaatgtac aatcaattag
      901 ccgcgccgat gcttgaagat ttacgcagcg tgatgcaaga agaaacgcaa agctttcttg
      961 ataagttcat tcaggatgcg gggtatcccg ttgacaagac gtttattgcc tacggtgagt
     1021 taagcgaaca tattctggaa gtatgtcaca aacatcattt cgatttggtg atttgcggta
     1081 atcacaatca cagtttcttt tcgcgagcat cctgctcggc gaaaagagtg attgcctcaa
     1141 gtgaggtcga tgtgctgtta gttccactta cgggagatta accgctccta cgcaagcttt
     1201 ggaaaggtag caactttatc gcgctgctgg ctttccgcgc ttcgcggaac tatctgcttt
     1261 aggtcgctaa tgaagcactc ctgccagtgg ttaatatcgt ttttcacgat aacgtccagc
     1321 atttctgcat gacgggaaat acgttccgcc agcgacatag tcaatgcacg atccagcgca
     1381 gctgcaactt cgtcacgatc gtaggggtta acaattaacg ccgacgttaa ctcgtttgcc
     1441 gctcccgcaa attgcgaaag aacaagaacg cccgggttgg ctgggtcctg agcagcaaca
     1501 tactcttttg ctaccaggtt catcccgtca cgcagtggcg tcactaagcc cacgtcagag
     1561 tagcggaata ttttcatcag taatttacgg tcaaaatgct gattcaaata ataaagcggc
     1621 gtccagccta attgcccgta tttaccatta attcgtccag cttcattttc gagctgatga
     1681 cgaatatcct gataggcttg cacatcacca cgcgacgttg gtgcaatctg ggtataacga
     1741 attttaccat gatgctgcgg atatttttcc agcaacgctt cataggcgag aaaacgctct
     1801 ggcaaacctt tggaataatc cagccgttcg acagaaaaga tattttgtac gtttttcagt
     1861 tccgctttaa gttgcgccag ttttggcggc agtggcccgg cagcctgttt ggctatttct
     1921 ttcggttcaa tgccgatcgg gtagacttct gttcgaaatg ctttgcccca ggctgtatgg
     1981 ctttttgcgc tacgtgtcgt gacgcgggtc aggttagaaa gacaatccag gaacgccaga
     2041 cgatcgtttt ctgtctggaa acccagcaaa tcataatcac aaagctgttc aagcaaggtg
     2101 tcatatgtcg gcagcgcgtt gaagatttcc ggtgtcggga aaggaatatg cagaaagaaa
     2161 ccaatgcgat tattcactcc ccgtttgcgt aattcatgcg caaatggcaa caggtgataa
     2221 tcgtggatcc agataatgtc atcgtcttgc aacagcggca gtaatttatc tgccagcaac
     2281 gcatttacgc gtagatagcc gtcccaggca ggacgctgaa attgcaccag atcgagccga
     2341 taatgaaaag cgggccagag aacggcattg gagaattggt tgtagtattc gtcaaggtcc
     2401 tgttcgctga ggttaaaaga ggcccacgta atgttacctt ttttcacctt ttttagcggc
     2461 tgatcctcat tccctgtttc accactccag ccaaaccaca gtccgcctgc ggctttcagt
     2521 gcccccagta tgccaacggc aaggccaccg gcactggcgg cgtgctcgtc tggtggtgca
     2581 atccggttag atactacgac taaacgactc atagtcatca ctcctgttat tttctctttt
     2641 ttgttgtaat gcggtggtta tcatttcaag ccagctccag acatccggca cacccgccag
     2701 tcgccatgat gcctgagttg cacctgtgcc aatttttact gacattccgc ccagtcggtt
     2761 aacgactgcg aagccagatt catcggttaa atcatcgccc agaaatacgg gcgttcgccc
     2821 gataaaggga gcttcctgca taaaagctgc aattgcctca cctttactgg tacctctcgg
     2881 tttgatctcg acaacacact ttccctgctg taacgccatt tgtggccaga tctgagtaat
     2941 acgttgcgct aatgtcatta atgcgtcttc atgctgcgga gcctgacgat aatgcagcgc
     3001 aaaagccatc cctttcgcct ccagctccgc gccgggatac tgagcgatga ctgtatgcag
     3061 ttgcacgcta atatcacgcg caatcgcatc cggcagatga acgatatgtg ttttaccatt
     3121 gatgtcacgg cgctccgccc catgcacgcc cgctaacggg aagcgataag gttttgccag
     3181 tgcgtcaagc tccaccattg agcgccctga tatcaatgcc aatgcaccat cacttgcggt
     3241 tgccagtagc tgtagtcctt gcagaatatt gtcaggcacg acgacctgat cgggatgcgg
     3301 tttgatttcc gccagcgttc catcaagatc aaaaaaccag gcatatttcg cggatagttc
     3361 aggggtttcg gttaacggtt ctgtcacccg gctctcctcc ttctcttttc ttattgtttt
     3421 gcgcagaacg cgctttacag acatgtaagt atagacagtg tgacgggggt cgccatttgc
     3481 aatgaagaac agccaaccgt tgtggcggct ggctgtggtg ggaaaaaacg ttaaattgtt
     3541 gtggaaaaaa gcatcagaca atgcgtttcg ctttttgctt gtaacggtcg aagatcaccg
     3601 ctgccagcag gattaagccg cgaaccacgt actgcgcgaa aggagaaata ttaagcaggt
     3661 tcatggcgtt ttccacggtg cctaaaatta agatacccgc caccacatat gagatttttc
     3721 cgatgccacc tttcagagaa acgccaccta aaacgcaggc ggagataaca ataagctcat
     3781 aaccaatcga cgtcattggc tgcccactag tcatacgtga agccagaata attccggcta
     3841 tcgcagatac caggccagag agaacaaaga taataatttt ggtgcgaaca accggtacac
     3901 ccgccagacg cgcagcctct tcgttcccgc caatcgccag ggtgttacga ccaaaggtgg
     3961 ttttattcag caacaaacca aagataatca gacacgcgac ggtgagccag attggcgcag
     4021 gcagaccgaa ccagttagcg taaccaaggg caaagaagct ttcatcttcg ataccgaccg
     4081 ctttaccgtc tgaaatgatg tacgccagac ctcgaacaat ctgcatcgtt gccagtgttg
     4141 tgatcagagc atttattttc agtttggcga taacaaagcc attgaccagg ccacagagaa
     4201 tgccaagcag caaccccgct gccacgccaa tccacaggct ttcagtcagg ttgataacca
     4261 ccgccgtggt gacacccgca caggcaatta cggaggcgac agaaaggtca aagtcaccgg
     4321 aagcaaggca gaacaacatg ccacacgcca ccatccccga catggaaatt gccaggccca
     4381 accctttcat attaatgaag gtggcaaaat ttgggacaaa aatggcacag gcgataaaga
     4441 gcaccgcaaa caccaccagc atgccgtact gatcccagat acgcccgaag ctgaatgacg
     4501 acttaggtgc gccagaaccc gatgtagaaa cagaagacat catactctcc ttactcaggc
     4561 aactgcctgg ctgactttag gcatcgcaag gctcagtgcc tgacgctcat ctgcctgctc
     4621 gtgtaacaat tcaccggcga tttcaccttc ccgcatcacc acaatccggt cggcaacgcc
     4681 gaggacttca ggtaagtcgc tggaggcaaa cagcaccgcc acaccctgcg ccgccagcgc
     4741 ataaattacg ttatatattt cgtgcttagc accaacatca atgctgcgcg taggttcatc
     4801 cagcaaaatg accttcatct cttccgataa ccagcggccc agaatggctt tttgctgatt
     4861 tccgcctgag agattcatga tcagttgctc agcgcccggc gttttgatgt tgagcgaacg
     4921 aatgtgctga tcggcattgt tttcttccca accgttgttg attacacaac cgccgagcac
     4981 atgtttgcgt ctggcactga tgttgatatt gtcgcgaacg gagtgcacgg gaataatgcc
     5041 ttccgctttg cgatcttccg ggcagagcat catgcctgcg gcaatggcgt ggctcggttt
     5101 acgaatatcg atcggctgtt ggtcgatata aacctgaccg gcggtgattt gcgtcccgcc
     5161 aaacaagcct ttcattaatt cgctacgccc cgcccctacc agaccaaaca gcccaacaat
     5221 ttcaccactg cgaaccgcca gacttattgg cgtacgcacg cctggtgctt tcacagcatc
     5281 aagacgcagg cgctcctcgc cataactacg aggttgccag ccgtagatat cgccaatgtc
     5341 gcgcccgacc atcgcctgca ccagcgcgtc gtggtcaacc tgctgcatat cggtaaaggt
     5401 tttgacataa cgtccatctt taaagacagt gatggcatcg ctgagggcaa atatttcttc
     5461 catacggtga gaaacgtata agatcacccg cccctctttt cgcagttcac gaataacgcg
     5521 gaaaagattg tcgatttcgc gggcagaaag ggagctggtt ggctcatcaa aggcgataat
     5581 tttagcgtta cgcgccagcg ccttggcgat ttcaaccatc tgccactgac caatggagag
     5641 atatttcagc ggcgtgtccg ggtcaatatc cataccaaga tgtttaagtt gtaaacccgc
     5701 ctcataattc agcaatgagc gattcacaat gccgccttta tgcggcagct ggccgagata
     5761 gatgttttcc gcgacggtca tttccggcac gagatgcagt tcctggtaaa taatcgccac
     5821 acccgcgtta agtgctgcgg tcgtgtcgga aaaggacatt tcctgcccat taatcactac
     5881 agaacccgtg gttggcgcat agttgccgct gaggattttt aagagagttg attttcctgc
     5941 gccattttca cccatcaacg catgaacctg accggcatag cagtcaaaac taatatccgt
     6001 cagcgcctta acgccgggaa acgttttacc gatgccgcga aatgagagat acggggtaga
     6061 ctgttgcata acgtctccgt gaatcactgg tcgttcacac cccctctgca tcatgcagag
     6121 gggaattttt ccggcaaatt acttaccgcc taaacctttt ttctccagtt cttctttaaa
     6181 gttgtcacgc gtgatcagca ccacgtcggt aacttcggta aattttggcg gttcaacgtc
     6241 ttttgctacc cagttgtaaa gcatttcgct ggatttatag ccatgtacgt ccgggcttgg
     6301 cagcagggaa ccgtagaagc cggttgcctg tgctttagac agttcgctca acgcatccac
     6361 accgttaatg ccaatgccga tgatatcggc agctttaaag ccctgacctt ccgtcgcgcg
     6421 tacgccgccc agcacggtgc tgtcgttcat accgacgatc agccaatgtt taacttccgg
     6481 atgttgaacc agcattgagt tggcagcgtc aaatgccccc gggatgtcgt tagatttggt
     6541 aggtacctga taaatttgtt tttccgggaa tccggccgct ttcagcgcat ccatagaacc
     6601 cgttgtacgg cgacgtgccg tatccagttc gttggcggta atcgccatca ccgcgctttc
     6661 tttgacatcc cagccacgtt tctgcatctc tttatacagt tcctgaccct gacgttcgcc
     6721 aattttagtc gccgccatca tcaccagcgg aacggtatcc attggcttac ctttggcgtt
     6781 aacaaactgg tcatccacgg caatgacttt catatcgtag ccacgcgctt tcgcgacgat
     6841 ggcagagccg agtttggggt ccggagtaca aataacgaaa ccttttgcgc cactggcagc
     6901 caggctgtcg atcgcgttca atgttttttc gccatccggc acggcaatct taataacctc
     6961 aaaccctaaa tccttcccgg ctttatcggc aaacttccat tcggtctgga accacggctc
     7021 ttccggttgc ttcaccagaa aaccgagctt caggttctcc gccatagcgg attgtgacat
     7081 aacggctgcc agaccaatgg ctgccagggc tttagtaaat ttgtgcatgg ttctctccag
     7141 ctttagtgtc gttttgtgta gggcaaaaac gaatgacttt cgttaaatta atcggaaaac
     7201 aaagcattac cttttatcta aaagataagt gaccgtgttg acatagtttt agcgagaaat
     7261 taattctcca taggagagca atatcacatc gcagaattac agtgagaacg tgcataaatt
     7321 tagcgggaaa agacataagg gaaagccaat ttgtcagaca aattgtcgaa tgcacagcag
     7381 attaatccat aagattagcc tggaaatcct tgttgtcttt ggtacccatg cgggatgtct
     7441 tctttttaac cagtcaatag gccgcattac ctggcgttga gtttttgaaa tggtgtaata
     7501 accgcaactc aaagatgtgg aaaatgcacg tcattcattt cgccattaat tatcactgtg
     7561 ctcattaatt aacagaacac gtataatgag agccatctcg caaaaatgaa aaaacgtttt
     7621 ataaaatcat cacttcatca tgaattcaaa ttcattgatt aatatcaaca agatacaaaa
     7681 agcactatca ttaaaattca ttgcagttac attgatttca tcaatgaaat gtaaaaatat
     7741 ataaacttga tgatttaagc attttcttat acccgttcag acgttattct tatttcagat
     7801 catcgtcaga attgactcca cgatcacatt tcggaccggc agaaaggaat tattctgcaa
     7861 acagtaatta tggtgttttg atttatcttg cacctctcca cttctggata taaggatatt
     7921 aggtatggca accgctggaa tgcttctcaa actcaactct caaatgaacc gcgagtttta
     7981 cgcatccaat ctctaccttc acctgagtaa ctggtgttct gaacagagtc tgaacggcac
     8041 cgccactttc cttcgcgccc aggcacagag taatgtgacc caaatgatgc gcatgtttaa
     8101 ctttatgaag agtgtcggcg ctacccccat cgttaaagcc attgatgttc ccggtgaaaa
     8161 actgaactct ctggaagaac tgttccaaaa aacgatggaa gaatacgagc aacgttctag
     8221 tacgttggca cagttagccg atgaagcgaa agaactgaat gatgattcaa ccgtcaattt
     8281 cctgcgcgat ctggaaaaag aacagcagca tgatggtctg ttgctgcaaa ccattcttga
     8341 tgaagtgcgc agtgcgaaac ttgcgggtat gtgccctgtg cagaccgacc aacatgttct
     8401 gaatgtcgtg tcacaccagc tgcattgatc atcatcggcg ctaatgcatt gcgccgatga
     8461 aggttttgag aaaccgctgc ctcatctgtt tgaagcagcg gtttttttaa tgggattcac
     8521 cctgtggggt aaacttgagt tcaataagcg cgatggcttt ttggattgcc cgcatggtga
     8581 ccgggtctgc ggcggcggga tggttagtaa agtcgatatt cttcagctga ctggacattt
     8641 tttcacgaac ttcaacgggc gcgattacat cgagaacatc cagaatttgt ttgataacca
     8701 actggcaagc aaccacatca gaaaccagtt cctgatcggc attcagcggc tgggacatcg
     8761 taaactcctg atagcatttt gaaagccgtt atagtagcgg cttcacatct tcagcgatag
     8821 tcacatccac cgtcatcagg acacaaaaaa acctgccgga gcaggttttt tgttatcgga
     8881 acatattgcc tggcggtacg tctttgaacg tcttgcaata gttattgaac atacttttca
     8941 ggattttgcg cagtttcatc gcggcactcc gaccatttgt tatacaggtg ttattgtctt
     9001 tgcgcttata atatgacaac catcacaaaa atcaatcttt atgtgataca aatcacataa
     9061 ataccccttt aatgttataa aaatgataat caaaaaacag cccccctatt tctgacacct
     9121 acagatggca agaaatagcg cctgccaggc gtcttttccg gccattgtcg cagcgctgta
     9181 acgcgtaaaa tagtgctttc tcttactctt ctggctggac catgagactt ctgattctga
     9241 ctctttcatt aataacgctg gcggggtgta cggttactcg ccaagcccat gtgagcgaag
     9301 ttgacgcggc aacaggtatt gtacggttgg tttatgacca ggcttttttg cagcatgccc
     9361 ataccgatcg ttatgtcagt cgcgggatcg ccgatcgcgc atgccagcag gaaggctata
     9421 cccacgcggt cccctttggt cagccagtag gcaactgtag cctttttgcg ggttccttat
     9481 gtttgaatac ggaattcact ttgtcatatc agtgccatca ttccgccttc cctgtctttc
     9541 tctaaatatt ttaatcagcg aggggatctt cgctgattaa agaaatagcg gtaatgcgtt
     9601 ttattcccat tcgtattttt aataattgaa gtttatattt taccttttgc aaataataaa
     9661 ataacaaatt atagtgacgc cacagcaaca aatataacct ttgtggagca ctatcatgct
     9721 gaaaccagaa atgattgaaa aacttaatga gcagatgaac ctggaactgt actcttcact
     9781 gctttatcag caaatgagcg cctggtgcag ctatcatacc ttcgaaggtg ctgccgcgtt
     9841 cctgcgccgt cacgcccagg aagagatgac gcatatgcag cgtctgtttg attacctgac
     9901 tgataccggc aatttaccgc gtattaatac cgttgaatct ccgtttgctg aatattcctc
     9961 acttgatgaa ttattccagg aaacctataa acacgaacaa ttaatcaccc agaaaattaa
    10021 cgaactggct catgctgcaa tgaccaatca ggactaccca acatttaatt tcctgcagtg
    10081 gtatgtttct gagcagcatg aagaagagaa actgttcaaa tcgattattg ataaattaag
    10141 cctggcaggc aaaagcggcg aaggtctgta ttttatcgac aaagaactct ctaccctcga
    10201 cacacaaaac taatgctctc cggcggcagg tttactgccg ccgtttcaaa tcagtgacgg
    10261 caaatcttac tctgatcggt cgaaaaaccg tctttcgaag gtataaattt gccttttgct
    10321 gccagaaacg ctaccagctc tcccgctgtc atcccttctg ccgagcaggt gtgaaaacgt
    10381 gcctgttcac caaaacgcgc tttaatcgca gcttccagac tggcatgcgt atattgctcg
    10441 cctgattcaa tcatcatatt taacacttca tgaccgtgaa tagagtccat cgttcctcct
    10501 caaaaaaagc ctagcgtagc gattgccgct tatgaagact ttgcgccagc gcaggactga
    10561 atgcttttta ttgtacattt atatttacac tatatgtaac gtcggtttga cgaagcagcc
    10621 gttatgcctt aacctgcgcc gcagatatca ctcataaaga tcgtcaggac agaagaaagc
    10681 gtgaaaaaca gaaccctggg aagtgttttt atcgtggcgg gaaccacaat tggcgcaggc
    10741 atgctggcaa tgccgctggc tgcggccggt gttggtttta gcgttacgtt aatcttgttg
    10801 attgggcttt gggcgttgat gtgctacacg gcgctattac tgctggaggt gtaccagcat
    10861 gttccggcag ataccggtct gggcacgctg gcaaaacgct atctgggacg ctacggtcaa
    10921 tggctgacgg gcttcagtat gatgttctta atgtatgctc tgactgcggc atacatcagc
    10981 ggtgtcggtg aattgttggc ctccagcatc agcgactgga caggtatttc aatgtcggca
    11041 accgctggcg tgctgttgtt cacttttgtt gccggtggcg tggtttgtgt tggaacatcg
    11101 ctggtcgatt tatttaaccg ttttctgttc agcgccaaaa ttatttttct ggtggtgatg
    11161 ctggtattgc tgctaccgca tattcacaaa gtgaatcttt taaccctgcc gttgcaacag
    11221 gggctggctc tgtcagcaat cccggtgatt tttacctcat ttggttttca cggtagcgtg
    11281 ccgagtattg tcagctatat ggatggcaac gttcgtaagc tacgctgggt gtttataacc
    11341 ggtagtgcga tccccctggt ggcatatatt ttctggcagg tggcaacgct tggcagcatt
    11401 gattcaacaa cctttatggg attgctggct aatcatgctg gattaaacgg gctgttacag
    11461 gcgttacgcg aaatggtggc ctctccgcat gttgagctgg cagtgcattt atttgctgat
    11521 ttagccctcg ccacgtcatt tctcggcgtt gcgttaggct tatttgatta tctggctgat
    11581 ttatttcagc gttcaaatac cgttggtgga cggttgcaaa ctggtgcaat tacctttctg
    11641 ccgccgttgg cgtttgcact gttttatcca cgaggatttg tgatggcgct gggttacgcc
    11701 ggtgtggcgc tggcggtact ggcattgatt atcccttcgc tgttgacctg gcaaagcaga
    11761 aagcacaatc ctcaggcggg ttaccgggtc aaaggtggtc gtccggcgct ggtggtggtg
    11821 tttctctgtg gtattgctgt gattggcgtg caatttttga ttgcggcagg gttgttacca
    11881 gaagtggggt gatcagatag cctcaaattc cttattgggt gccagaatta acgctgacac
    11941 ccaatttggc ctcttaatgc aggcagcact gcttaaattt cttaccacta ccgcacgggc
    12001 aaggatcgtt acgccccggt ttctcttctg ctttgatcgg ttgctgaaca gctttttcct
    12061 gcggatgcgc catccagtac gcatgtagat caagcgccgc cagtcgaatg gcatctacgc
    12121 tctcttcaaa cgcttctggc gacatctttt ctacccgctc gaagttttcc tcagtaccgt
    12181 gcagcgcaat cgcctccagc gctggtttta acgaatcggg caacgttgac cagtcagaaa
    12241 gtgccacgcc ccgcatatag ccaaagcacc actcctcaac aatcgtcagc tcgctgccat
    12301 caacttctcg caagccgaat aacggctcaa actgctccgg gaattcgttc agacgctctg
    12361 cggtatcggc catatgttga aaagccaggt tcataaagcg cgtcatctct ttctctgacg
    12421 cccagcgcgg cacatagtca gccccacccc acacggcaac cagccactgt tccggttcaa
    12481 tctcttgcgg agaactcaac accgccgtca ataaaccgtc cagctccgcc acatcaagga
    12541 tggcgtggtt agtgttgtat ttggtcagaa tatcgtccag ccattccaac tcactttcgt
    12601 ttaacggtcc cgttttcata cgcttttcct tgtggatctc aactcgccag cacctatctt
    12661 acatgccggt ccgtatcaga gatacttttt gagtggcttt gccggtgatt aaaaattaag
    12721 gagggtgtaa cgacaagttg caggcacaaa aaaaccaccc gaaggtggtt tcacgacact
    12781 gcttattgct ttgattttat tcttatcttt cccatggtac ccggagcggg acttgaaccc
    12841 gcacagcgcg aacgccgagg gattttaaat cccttgtgtc taccgattcc accatccggg
    12901 ctcgggaaga aagtggaggc gcgttccgga gtcgaaccgg actagacgga tttgcaatcc
    12961 gctacataac cgctttgtta acgcgccaaa ttcttcaggc ctttcagcca gacatccgct
    13021 tgacgccgat gtcttttaaa
//
LOCUS       DAJTZL010000119        12996 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.119, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000119 DAJTZL010000000
VERSION     DAJTZL010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12996
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.119"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            96..1391
                     /locus_tag="OO544_002904"
     CDS             96..1391
                     /locus_tag="OO544_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HCR6768444.1"
                     /translation="MDADSSDSTVTPGRLPDGLSSCPCFYLGKKLMNLSANAQATLLL
                     TSDFSRAAASEYKPLSNSEWGKFALWLKHQRISPAELLVPQPQEKLTGWSDPRISQER
                     ILGLLARGHSLALAVDKWQRAGLWILTRGDADYPVRLKNRLRTDAPPVLFGCGNKALL
                     QAEGMAIVGSRDAPTDDLRYTQQLAAKLAQQGICVISGGARGIDECAMASALEAGGTA
                     VGVLADSLLKTSTLVKWREGLIAGNLVLISPFYPEVRFTVGNAMARNKYIYCLAESAM
                     VVRAGMTGGTITGAMEALKHQWLPVQVKPNQDMQSANSRLVENGASWSAEQAENVTIR
                     LPDVPGLMYDRALRNAQPELFSLHEDDANYAVMPAYTPVDFYQLFVAELAILAKESIS
                     IERLASCTGLTIEQISVWLNRAEEEGRVIRLGEGHYQFR"
     gene            complement(1437..2030)
                     /gene="yhgN"
                     /locus_tag="OO544_002905"
     CDS             complement(1437..2030)
                     /gene="yhgN"
                     /locus_tag="OO544_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HCR6768445.1"
                     /translation="MNAIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            2222..3325
                     /gene="asd"
                     /locus_tag="OO544_002906"
     CDS             2222..3325
                     /gene="asd"
                     /locus_tag="OO544_002906"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCR6768446.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            3598..5784
                     /gene="glgB"
                     /locus_tag="OO544_002907"
     CDS             3598..5784
                     /gene="glgB"
                     /locus_tag="OO544_002907"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HCR6768447.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPVYRQYHHDKLT
                     FGILYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            5781..7754
                     /gene="glgX"
                     /locus_tag="OO544_002908"
     CDS             5781..7754
                     /gene="glgX"
                     /locus_tag="OO544_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HCR6768448.1"
                     /translation="MTRLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIEG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALHEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYACACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIALGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGSLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            7772..9067
                     /gene="glgC"
                     /locus_tag="OO544_002909"
     CDS             7772..9067
                     /gene="glgC"
                     /locus_tag="OO544_002909"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HCR6768449.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            9067..10500
                     /gene="glgA"
                     /locus_tag="OO544_002910"
     CDS             9067..10500
                     /gene="glgA"
                     /locus_tag="OO544_002910"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HCR6768450.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDNNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            10519..12966
                     /gene="glgP"
                     /locus_tag="OO544_002911"
     CDS             10519..12966
                     /gene="glgP"
                     /locus_tag="OO544_002911"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HCR6768451.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEMR
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
BASE COUNT         3040 a         3228 c         3576 g         3152 t
ORIGIN      
        1 ttccaccagt gtcaaaatct cgacggggcg tttgttatta cccctccttt gatgcctggt
       61 ccggcgttgc tggttgacga tatcgtggat tctgcatgga cgctgacagt tctgacagca
      121 ctgttacgcc aggcaggttg cccgacggtt tatcctcttg cccttgcttc tacctcggta
      181 aaaaattgat gaatctttca gccaatgcac aagcaactct cctgctaacc agcgattttt
      241 ctcgcgcggc ggcgagtgag tataaacctc ttagtaatag tgaatggggg aagtttgcat
      301 tatggctgaa gcaccaacgt atcagtcccg ccgagcttct ggtgccgcaa ccgcaagaga
      361 aacttacagg ctggagcgat ccgcgtattt ctcaggagcg tattcttggc ttgctggcgc
      421 gtggtcatag tctggcgttg gcggtagata agtggcaacg cgccggttta tggatcttaa
      481 cccgcggaga tgctgattat cccgttcgct tgaaaaaccg attgcgaacg gatgcacctc
      541 ccgttttatt tggctgcggg aataaagcat tactgcaagc ggaaggtatg gcgattgttg
      601 gctcgcgaga tgctccgact gacgatttgc gctataccca acaactggcc gcgaaactgg
      661 cccaacaggg gatttgcgtt atctctggtg gtgcgcgagg tattgatgaa tgtgcaatgg
      721 cgtcggcact ggaggccggg ggaactgccg ttggcgtatt agctgatagc ttgttaaaaa
      781 cgagtacgtt agtgaaatgg cgtgaagggc ttatagcagg caacctggtg ttgatttcgc
      841 cgttttaccc agaggtacgt ttcaccgtcg gcaatgcgat ggcgcgaaat aaatatattt
      901 attgccttgc tgaaagcgca atggttgtac gtgcgggaat gaccggtgga acgataaccg
      961 gggcgatgga ggcattaaaa catcagtggc tgcctgtgca ggttaaacca aatcaggata
     1021 tgcaatcagc caattcacga ttagtagaaa atggggcgtc atggagtgct gaacaggctg
     1081 agaatgtgac gatcagactg ccagacgttc ctgggctgat gtatgacaga gcactccgta
     1141 acgctcaacc agaactgttt tcgctgcatg aagatgacgc aaattacgca gtaatgcccg
     1201 cgtatacgcc tgtcgatttt tatcaactct ttgtggcgga actggcgatc cttgcaaagg
     1261 aatcgataag tattgaaagg ctggcgtctt gtactggttt aaccatcgaa caaattagtg
     1321 tgtggctgaa ccgcgcagaa gaagagggaa gggttatccg attgggcgaa ggtcattatc
     1381 agttcaggta ataaaacagg tgccgcgtct ggttgtgtgc agcacctgtt tgaaccttac
     1441 cccttcatcc acattcgaat gccgtcgagg aacatctggg ttgccatcat caccagaatc
     1501 aatcccatca ggcgttcaag tgcgttcacc cctttctcgc ccagcagacg taaaaatagc
     1561 gaagactgta gcaggatgac aaaggtgccg ccccaggcca gcagcagagc aatcaccaga
     1621 tgtcccatct gattcgggta ctgatgagac aacaacatca gcgtggcgag aatagtcggc
     1681 ccggcgacta acggaattgc caacggcacg ataaatggct cttcacctgc cggaagcccg
     1741 ctgctatttc ctgaagcgct ggggaaaatc atcttaatgg cgattagaaa cagaatgatg
     1801 ccgccagaaa tggagacggt ttctgctcgc aggctgagaa atgccagaat tttctcaccc
     1861 gcaaacagga acaccagcat caccaggaga gcaataagca actctcgcac catgattgcc
     1921 cgccgtcttt tcggttcagt atgtttcagt acggacatga aaataggcag gtttccgagc
     1981 ggatccataa tcaggatcaa taaaactgct gcagaaatga ttgcattcat aactcaaatt
     2041 ccctgataat tgccgcggac tttctgcgtg ctaacaaagc aggataagtc gcattactga
     2101 tggcttcgct atcattgatt aatttcactt gcgactttgg ctgctttttg tatggtgaaa
     2161 gatgtgccaa gaggagaccg gcacatttat acagcacaca tctttgcagg aaaaaacgct
     2221 tatgaaaaat gttggtttta tcggctggcg cggtatggtc ggctccgttc tcatgcaacg
     2281 catggttgaa gagcgcgact tcgacgccat tcgccctgtc ttcttttcta cttctcagct
     2341 tggccaggct gcgccgtctt ttggcggaac cactggcaca cttcaggatg cctttgatct
     2401 ggaggcgcta aaggccctcg atatcattgt gacctgtcag ggcggcgatt ataccaacga
     2461 aatctatcca aagcttcgtg aaagcggatg gcaaggttac tggattgacg cagcatcgtc
     2521 tctgcgcatg aaagatgacg ccatcatcat tcttgacccc gtcaatcagg acgtcattac
     2581 cgacggatta aataatggca tcaggacttt tgttggcggt aactgtaccg taagcctgat
     2641 gttgatgtcg ttgggtggtt tattcgccaa tgatcttgtt gattgggtgt ccgttgcaac
     2701 ctaccaggcc gcttccggcg gtggtgcgcg acatatgcgt gagttattaa cccagatggg
     2761 ccatctgtat ggccatgtgg cagatgaact cgcgaccccg tcctctgcta ttctcgatat
     2821 cgaacgcaaa gtcacaacct taacccgtag cggtgagctg ccggtggata actttggcgt
     2881 gccgctggcg ggtagcctga ttccgtggat cgacaaacag ctcgataacg gtcagagccg
     2941 cgaagagtgg aaagggcagg cggaaaccaa caagatcctc aacacatctt ccgtaattcc
     3001 ggtagatggt ttatgtgtgc gtgtcggggc attgcgctgc cacagccagg cattcactat
     3061 taaattgaaa aaagatgtgt ctattccgac cgtcgaagaa ctgctggctg cgcacaatcc
     3121 gtgggcgaaa gtcgttccga acgatcggga aatcactatg cgtgagctaa ccccagctgc
     3181 cgttaccggc acgctgacca cgccggtagg ccgcctgcgt aagctgaata tgggaccaga
     3241 gttcctgtca gcctttaccg tgggcgacca gctgctgtgg ggggccgcgg agccgctgcg
     3301 tcggatgctt cgtcaactgg cgtaatcttt attcattaaa tctggggcgc gatgccgccc
     3361 ctgttagtgc gtaatacagg agtaagcgca gatgtttcat gatttaccgg gagttaaata
     3421 gagcattggc tattctttaa gggtggctga atacatgagt attcacagcc ttacctgaag
     3481 tgaggacgac gcagagagga tgcacagagt gctgcgccgt tcaggtcaaa aaaatgtcac
     3541 aaccagaagt caaaaatcca attggatggg gtgacacaat aaaacaggaa gacaagcatg
     3601 tccgatcgta tcgatagaga cgtgattaac gcgctaattg caggccattt tgcggatcct
     3661 ttttccgtac tgggaatgca taaaaccacc gcgggactgg aagtccgtgc ccttttaccc
     3721 gacgctaccg atgtgtgggt gattgaaccg aaaaccgggc gcaaactcgc aaaactggag
     3781 tgtctcgact cacggggatt ctttagcggc gtcattccgc gacgtaagaa ttttttccgc
     3841 tatcagttgg ctgttgtctg gcatggtcag caaaacctga ttgatgatcc ttaccgtttt
     3901 ggtccgctaa tccaggaaat ggatgcctgg ctattatctg aaggtactca cctgcgcccg
     3961 tatgaaacct taggcgcgca tgcagatact atggatggcg tcacaggtac gcgtttttct
     4021 gtctgggctc caaacgcccg tcgggtctcg gtggttgggc aattcaacta ctgggacggt
     4081 cgccgtcacc cgatgcgcct gcgtaaagag agcggcattt gggaactgtt tatccctggg
     4141 gcgcataacg gtcagctcta taaatacgag atgattgatg ccaatggcaa cttgcgtctg
     4201 aagtccgacc cttatgcctt cgaagcgcaa atgcgcccgg aaaccgcgtc tcttatttgc
     4261 gggctgccgg aaaaggttgt acagactgaa gagcgcaaaa aagcgaatca gtttgatgcg
     4321 ccaatctcta tttatgaagt tcacctgggc tcctggcgtc gccacaccga taacaatttc
     4381 tggttgagct accgcgagct ggccgatcaa ctggtgcctt atgctaaatg gatgggcttt
     4441 acccacctcg aactactgcc cattaacgag catcccttcg atggcagttg gggttatcag
     4501 ccaaccggcc tgtatgcacc aacccgccgt tttggtactc gcgacgactt ccgttatttc
     4561 attgatgccg cacacgcagc gggtctgaac gtgattctcg actgggtgcc aggccacttc
     4621 ccgaccgatg actttgcgct tgccgaattt gatggcacga acttgtatga acacagcgat
     4681 ccgcgcgaag gctatcatca ggactggaat acgctgatct acaactatgg tcgccgtgaa
     4741 gtcagtaact tcctcgtcgg taacgcgctt tactggattg aacgttttgg tattgatgcg
     4801 ctgcgcgtcg atgcggtggc gtcaatgatt tatcgcgact acagccgtaa agagggggag
     4861 tggatcccga acgaatttgg cgggcgcgag aatcttgaag cgattgaatt cttgcgtaat
     4921 accaaccgta ttcttggtga gcaggtttcc ggtgcggtga caatggctga ggagtctacc
     4981 gatttccctg gcgtttctcg tccgcaggat atgggcggtc tgggcttctg gtacaagtgg
     5041 aacctcggct ggatgcatga caccctggac tacatgaagc tcgacccggt ttatcgtcag
     5101 tatcatcacg ataaactcac cttcgggatt ctctacaact acactgaaaa cttcgtcctg
     5161 ccgttgtcgc atgatgaagt ggtccacggt aaaaaatcga ttctcgaccg catgccgggc
     5221 gacgcatggc agaaattcgc taacctgcgc gcctactatg gctggatgtg ggcattcccg
     5281 ggcaagaaac tgctgttcat ggggaacgaa tttgcccaag gccgtgagtg gaaccatgac
     5341 gccagcctcg actggcatct gttggaaggc ggcgataact ggcaccacgg tgtccagcgt
     5401 ctggtgcgcg atctgaacct cacctaccgc caccataaag caatgcatga actggatttt
     5461 gacccgtacg gctttgaatg gctagtggtg gatgacaaag aacgctcggt gctgatcttt
     5521 gtgcgtcgcg ataaagaggg taacgaaatt atcgttgcca gtaactttac gccggtgccg
     5581 cgtcatgatt atcgcttcgg cattaatcag ccaggcaaat ggcgtgaaat cctcaatacc
     5641 gattccatgc actatcacgg cagtaatgca ggcaatggcg gcacggtaca cagcgatgag
     5701 attgccagcc acggtcgtca gcattcacta agcctgacac taccaccgct ggccactatc
     5761 tggctggttc gggaggcaga atgacacgac tcgccattgg caaacccgct cccctcggcg
     5821 cgcattacga cggtcagggc gtcaacttca cacttttctc cgctcatgcc gagcgggtag
     5881 agctgtgtgt ctttgacgcc aatggccagg aacatcgcta tgacttgcca gggcacagtg
     5941 gcgacatttg gcacggttat ctgccggatg cgcgcccggg tttgcgttat ggttatcgcg
     6001 ttcatggccc ctggcaaccc gccgaggggc atcgctttaa cccggcgaag ttgttgattg
     6061 atccttgcgc gcggcaaatt gaaggggaat tcaaagataa cccgctgctg cacgccggtc
     6121 ataatgaacc tgactatcgc gacaacgccg ccattgcgcc gaaatgcgta gtggtggttg
     6181 atcactatga ctgggaagat gatgccccgc cgcgcacgcc gtggggcagc accatcattt
     6241 atgaagccca tgttaaagga ttaacgtacc tgcacccgga gatcccggtc gagatccgtg
     6301 gcacttataa agccctcggg catccggtga tgatcaacta tttgaaacag ttgggcatta
     6361 ccgcgctgga actgctgccg gtggcgcagt ttgccagtga accacgtctg caacgcatgg
     6421 ggctaagtaa ctactggggt tacaacccgg tggcgatgtt tgcgctgcat ccggcgtatg
     6481 cctgctcgcc agaaacggcg ctgcatgagt ttcgcgatgc aatcaaagca ctgcataaag
     6541 cgggtatcga agtcattctt gatatcgtgc tcaaccatag tgcggaactg gacctcgacg
     6601 gcccgttatt ctcgctgcgt gggatcgaca accgtagcta ttattggata agagaagacg
     6661 gcgattatca caactggacc ggttgtggca acacgctcaa tttgagtcat ccggcggtgg
     6721 tggattatgc ctgcgcctgc ctgcgttatt gggtagaaac ctgtcacgtc gatggtttcc
     6781 gctttgatct ggcggcagtc atgggccgta cgccagagtt ccgtcaggat gcgccgttgt
     6841 ttaccgctat ccagaactgc ccggtgctct cgcaggtgaa gttaattgct gaaccgtggg
     6901 atatcgccct tggtggttat caggtgggaa atttcccgcc gctgtttgcc gagtggaacg
     6961 atcatttccg cgatgctgcc cgtcgtttct ggctacatta tgatttgcct ctgggggcgt
     7021 ttgccgggcg ttttgctgcc tccagcgatg tttttaaacg taatggtcgt ctgccgagtg
     7081 ccgcgattaa tctcgtcact gcgcatgacg gttttacgct tcgcgactgc gtttgcttca
     7141 accataaaca caatgaagca aacggagaag aaaatcgcga cgggaccaac aacaattaca
     7201 gtaacaatca tggtaaagaa gggttaggcg gttctcttga cctggttgaa cggcggcgcg
     7261 acagtattca cgccctgtta acaacgttgt tgctctccca gggtacgccg atgttactgg
     7321 ccggtgacga acacggtcac agccagcatg gcaataacaa tgcctactgt caggataacc
     7381 aattaacctg gttggactgg tcgcaggcaa gcagtggttt aaccgcattt accgccgcgt
     7441 taatccatct gcgcaagcgc attcccgctt tggtggagaa tcgctggtgg gaagaaggcg
     7501 acggcaatgt ccgttggcta aatcgatatg ctcaaccttt aagcacggat gagtggcaaa
     7561 acgggccgaa acagctgcaa attctgctct cggatcgctt tttgatcgca attaacgcca
     7621 cgcttgaggt aacagagatt gttttacctg ctggggagtg gcacgccatt cccccattcg
     7681 ctggagagga taacccagtg attacggctg tctggcaggg acctgcacac ggattgtgtg
     7741 tgttccagag atgataaaaa aggagttagt catggttagt ttagagaaga acgatcactt
     7801 aatgttggcg cgccagctgc cattgaaatc tgttgccctg atactggcgg gaggacgtgg
     7861 tacccgcctg aaggatttaa ccaataagcg agcaaaaccg gccgtacact tcggcggtaa
     7921 gttccgcatt attgactttg cgctgtctaa ctgcatcaac tctgggatcc gtcgtatggg
     7981 cgtgatcacc cagtaccagt cccacactct ggtgcagcac attcagcgcg gctggtcatt
     8041 cttcaatgaa gaaatgaacg agtttgtcga tctgctgcca gcacagcaga gaatgaaagg
     8101 ggaaaactgg tatcgcggca ccgcagatgc ggtcacccaa aacctcgaca ttatccgccg
     8161 ttataaagcg gaatacgtgg tgatcctggc gggcgaccat atctacaagc aagactactc
     8221 gcgtatgctt atcgatcacg tcgaaaaagg cgcacgttgc accgttgctt gtatgccagt
     8281 accgattgaa gaagcctccg catttggcgt tatggcggtt gatgagaacg ataaaattat
     8341 cgaattcgtt gaaaaacctg ctaacccacc gtcaatgccg aacgatccga gcaaatctct
     8401 ggcgagtatg ggtatctacg tctttgacgc cgactatctg tatgaactgc tggaagaaga
     8461 cgatcgtgat gagaactcca gccacgactt tggcaaagat ttgattccca agatcaccga
     8521 agccggtctg gcctatgcgc acccgttccc gctctcttgc gtacaatccg acccggatgc
     8581 cgagccgtac tggcgcgatg tgggtacgct ggaagcttac tggaaagcga acctcgatct
     8641 ggcctctgtg gtgccggaac tggatatgta cgatcgcaat tggccaattc gcacctacaa
     8701 tgaatcatta ccgccagcga aattcgtgca ggatcgctcc ggtagccacg ggatgaccct
     8761 taactcactg gtatccggcg gctgtgtgat ctccggttcg gtggtggtgc agtccgttct
     8821 gttctcgcgc gttcgcgtga attcattctg caacattgat tccgccgtat tgttaccgga
     8881 agtatgggta ggtcgctcgt gccgtctgcg ccgctgcgtc atcgatcgtg cttgtgttat
     8941 tccggaaggc atggtgattg gtgaaaacgc agaggaagat gcacgtcgtt tctatcgttc
     9001 agaagaaggc atcgtgctgg taacgcgcga aatgctacgg aagttagggc ataaacagga
     9061 gcgataatgc aggttttaca tgtatgttca gagatgttcc cgctgcttaa aaccggcggt
     9121 ctggctgatg ttattggggc attacccgca gcacaaatcg cagacggcgt tgacgctcgc
     9181 gtactgttgc ctgcatttcc cgatattcgc cgtggcgtga ccgatgcgca ggtagtatcc
     9241 cgtcgtgata ccttcgccgg acatatcacg ctgttgttcg gtcattacaa cggggttggc
     9301 atatacctga ttgacgcgcc gcatctctat gatcgtccgg gaagcccgta tcacgatacc
     9361 aacttatttg cctataccga caacgtattg cgttttgcgc ttctggggtg ggttggggca
     9421 gaaatggcca gcgggcttga cccattctgg cgtcctgatg tggtgcatgc gcacgactgg
     9481 catgcaggcc ttgcgcctgc gtatctggcg gcgcgcgggc gtcctgcgaa gtcggtgttt
     9541 actgtgcaca acctggccta tcaaggcatg ttttatgcac atcacatgaa tgacatccaa
     9601 ttgccatggt cattctttaa tattcatggg ctggaattca acggacaaat ctctttcctg
     9661 aaggccggtc tgtactatgc cgatcacatt acggcggtca gtccaaccta cgctcgcgag
     9721 atcaccgaac cgcagtttgc ctacggtatg gaaggtctgt tgcaacagcg tcatcgtgaa
     9781 gggcgtcttt ccggcgtact gaacggcgtg gacgagaaaa tctggagtcc agagacggac
     9841 ttactgttgg cctcgcgcta cacccgcgat acgttggaag ataaagcgga aaataagcgc
     9901 cagttacaaa tcgcaatggg gcttaaggtt gacgataaag tgccgctttt tgcagtggtg
     9961 agccgtctga ccagccagaa aggtctcgac ctggtgctgg aagccttacc gggtcttctg
    10021 gagcagggcg ggcagctggc gctactcggc gcgggcgatc cggtgctgca ggaaggtttc
    10081 cttgcggcgg cagcggaata ccccggtcag gtgggcgttc agattggcta tcacgaagca
    10141 ttttcgcatc gcattatggg cggcgcggac gtcattctgg tgcccagccg ttttgaaccg
    10201 tgcggcttaa cgcaacttta tggattgaag tacggtacgc tgccgttagt gcggcgcacc
    10261 ggtgggcttg ctgatacggt ttctgactgt tctcttgaga accttgcaga tggcgtcgcc
    10321 agtgggtttg tctttgaaga taataatgcc tggtcgctgt tacgggctat tcggcgtgct
    10381 tttgtactgt ggtcccgtcc ttcactgtgg cggtttgtgc aacgtcaggc tatggcaatg
    10441 gattttagct ggcaggtcgc ggcgaagtcg taccgtgagc tttactatcg cttgaaatag
    10501 ttttcaggaa acgcctatat gaatgctccg tttacatatt catcgcccac gcttagcgta
    10561 gaagctctta agcactctat cgcttacaag ctgatgttta cgattggaaa ggacccggtc
    10621 gtcgccaata aacatgaatg gctgaacgca acgttatttg ctgtgcgcga tcgtctcgtg
    10681 gagcgctggt tacgttcaaa ccgtgcccag ttgtcgcaag aaacacgtca ggtttactac
    10741 ctgtcgatgg agtttttgat tggccgtacg ctctccaacg ccatgttgtc gctaggaatt
    10801 tacgaagatg tacagggcgc actggaagcg atggggttaa atctcgaaga gctgattgat
    10861 gaagaaaatg acccaggcct cggtaacggt ggcctgggac gtctggcggc ttgcttcctt
    10921 gattctctgg cgacgttagg gttgccgggg cgcggttacg gcatccgcta tgactacggt
    10981 atgttcaagc agaacatcgt taacggtagc cagaaagagt cgccagacta ctggctggaa
    11041 tacggtaacc cgtgggaatt caaacgccac aacacgcgct ataaagtccg ttttggcggt
    11101 cgcattcagc aggaaggtaa aaaaacgcgc tggattgaaa ccgaagagat tctgggagtc
    11161 gcttacgatc agataatccc tggttacgac accgacgcga ccaacacgct gcgtttgtgg
    11221 agtgcgcaag ccagtagcga aattaacctc ggtaaattca accagggtga ctacttcgcg
    11281 gcagtggaag ataaaaacca ctccgagaac gtatctcgcg tactgtatcc ggatgactcc
    11341 acctactccg ggcgtgagct gcgcctgcgt caggaatact tcctggtttc ctcgaccatt
    11401 caggacattt taagccgcca ttatcagttg cataaaacct acgataacct ggcggataaa
    11461 atcgcgattc atctcaatga tacccatccg gtactgtcga ttcctgagat gatgcgtctg
    11521 ctgatcgatg agcaccaatt tagctgggac gacgcgtttg aggtgtgttg tcaggtcttc
    11581 tcctacacta accacacgct gatgagcgag gcgctggaaa cctggccggt tgatatgctg
    11641 ggtaaaattc tgccgcgtca cctgcagatc atctttgaaa tcaacgacta tttcctgaaa
    11701 accttgcagg aacagtatcc gaacgatacc gatctgctgg gacgggcgtc gatcattgat
    11761 gaatccaacg gtcgtcgtgt gcgtatggcc tggctggcgg ttgttgtgag ccacaaagtt
    11821 aacggtgtat cggaactgca ctctaatctg atggtgcaat cgttgtttgc cgactttgcg
    11881 aaaatcttcc cgggtcgttt caccaacgtc accaacggtg tgacgccgcg tcgctggctg
    11941 gcggtagcga acccatcgct ttcagccgtg ctggacgaac acctgggccg taactggcgc
    12001 accgacctta gcctgcttaa tgagctgcaa caacactgtg atttcccaat ggttaatcac
    12061 gctgtgcatc aggcgaagct ggagaacaaa aagcgtctgg cagagtatat cgcccagcag
    12121 ctgaatgtgg tggtgaatcc aaaggcgttg ttcgatgtac aaatcaaacg tattcacgaa
    12181 tacaaacgtc aattgatgaa tgtgttgcat gtgattaccc gctataaccg catcaaggcc
    12241 gacccggatg cgaagtgggt accgcgcgtg aatatttttg gcggtaaggc ggcttcggcc
    12301 tattacatgg cgaagcacat tattcatttg atcaatgacg tagcgaaagt gatcaacaac
    12361 gatccgcaga ttggcgataa gctgaaagtc gtgttcatcc cgaactacag cgttagcctg
    12421 gcgcagttga tcattccggc ggcagatctg tctgaacaga tttcgctggc agggacggaa
    12481 gcttccggca ccagtaacat gaagtttgcg cttaacggtg cgctgactat cggtacgttg
    12541 gacggtgcga atgtcgagat gcgggatcat gtcggtgctg acaatatctt tatttttggt
    12601 aacacagcgg aagaagtgga agaactgcgt cgtcagggct acaaaccgcg tgaatactac
    12661 gagaaagatg aggagctgca tcaggtgctg acgcaaatcg gcagcggtgt attcagtccg
    12721 gaagatccgg gtcgctatcg cgatctggtt gattcgctga tcaacttcgg cgatcactat
    12781 caggttctgg cggattatcg cagctatgtc gattgtcagg ataaagtcga cgaactctac
    12841 gagcttcagg aagagtggac cgcaaaagcg atgctgaaca ttgccaatat gggctacttc
    12901 tcttctgacc gtactatcaa agagtacgcc gatcatatct ggcatatcga tccggtgaga
    12961 ttgtaagttc accaataaat agaacggggc caaagg
//
LOCUS       DAJTZL010000120        12627 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.120, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000120 DAJTZL010000000
VERSION     DAJTZL010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12627
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.120"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..2349
                     /gene="napA"
                     /locus_tag="OO544_002912"
     CDS             <1..2349
                     /gene="napA"
                     /locus_tag="OO544_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HCR6768452.1"
                     /translation="RFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKI
                     MYGKDRLTQPLLRMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGS
                     GQWTIWEGYAASKLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQA
                     DAFVLWGANMAEMHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSD
                     LVILNYIANYIIQNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASE
                     PMSFEDYKAFVAEYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRG
                     VWANNLVYNLHLLTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKH
                     RDICEKKWNIPSGTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMP
                     GWRDPRNFIIVSDPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGE
                     AKSDLWQLVQFSRRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAED
                     QLNDESRELGFYLQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRY
                     SEGNDPYVKAGEGYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTG
                     SMTRRVPELHRAFPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPP
                     QGLVYMPFFDAAQLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            2356..3051
                     /gene="napG"
                     /locus_tag="OO544_002913"
     CDS             2356..3051
                     /gene="napG"
                     /locus_tag="OO544_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HCR6768453.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            3038..3901
                     /gene="napH"
                     /locus_tag="OO544_002914"
     CDS             3038..3901
                     /gene="napH"
                     /locus_tag="OO544_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HCR6768454.1"
                     /translation="MANRKRDAGREALVKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSCSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILLGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            3898..4347
                     /gene="napB"
                     /locus_tag="OO544_002915"
     CDS             3898..4347
                     /gene="napB"
                     /locus_tag="OO544_002915"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HCR6768455.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            4357..4959
                     /gene="napC"
                     /locus_tag="OO544_002916"
     CDS             4357..4959
                     /gene="napC"
                     /locus_tag="OO544_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HCR6768456.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            4978..5595
                     /gene="ccmA"
                     /locus_tag="OO544_002917"
     CDS             4978..5595
                     /gene="ccmA"
                     /locus_tag="OO544_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HCR6768457.1"
                     /translation="MLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTTLL
                     RLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHRDG
                     DTAQCLEALAQAGLAGFDDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAIDVN
                     GVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            5592..6254
                     /gene="ccmB"
                     /locus_tag="OO544_002918"
     CDS             5592..6254
                     /gene="ccmB"
                     /locus_tag="OO544_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HCR6768458.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            6296..7033
                     /gene="ccmC"
                     /locus_tag="OO544_002919"
     CDS             6296..7033
                     /gene="ccmC"
                     /locus_tag="OO544_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HCR6768459.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            7030..7239
                     /gene="ccmD"
                     /locus_tag="OO544_002920"
     CDS             7030..7239
                     /gene="ccmD"
                     /locus_tag="OO544_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HCR6768460.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            7236..7715
                     /gene="ccmE"
                     /locus_tag="OO544_002921"
     CDS             7236..7715
                     /gene="ccmE"
                     /locus_tag="OO544_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HCR6768461.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMLGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            7712..9655
                     /gene="ccmF"
                     /locus_tag="OO544_002922"
     CDS             7712..9655
                     /gene="ccmF"
                     /locus_tag="OO544_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HCR6768462.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMIASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASYSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSIDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            9652..10209
                     /gene="dsbE"
                     /locus_tag="OO544_002923"
     CDS             9652..10209
                     /gene="dsbE"
                     /locus_tag="OO544_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HCR6768463.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            10206..11258
                     /gene="ccmH"
                     /locus_tag="OO544_002924"
     CDS             10206..11258
                     /gene="ccmH"
                     /locus_tag="OO544_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HCR6768464.1"
                     /translation="MRFLLGMLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVAYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIDGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(11293..11940)
                     /gene="narP"
                     /locus_tag="OO544_002925"
     CDS             complement(11293..11940)
                     /gene="narP"
                     /locus_tag="OO544_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HCR6768465.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            12260..>12627
                     /locus_tag="OO544_002926"
     CDS             12260..>12627
                     /locus_tag="OO544_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR6768466.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVGLE
                     KDIQLVYGTANNTKINPGGEQHIKEFGVSSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAVNQTTLNNGVLQVYG"
BASE COUNT         2685 a         3169 c         3738 g         3035 t
ORIGIN      
        1 cgtttctgcg gtactggttg cggcgttctg gtcggaacgc agcaggggcg tgtggtggcc
       61 tgtcagggcg acccggacgc accggttaac cgtggcctga actgcattaa gggctatttc
      121 ctgcccaaga tcatgtacgg taaagaccgt ttgacgcagc cgctgctgcg tatgaaaaac
      181 ggtaaatatg acaaagaagg cgaatttacc ccaatcacct gggatcaggc cttcgatgtg
      241 atggaagaga aattcaaaac cgccctgaaa gaaaaagggc cggaatcgat cggtatgttc
      301 ggttctggtc agtggactat ctgggaaggt tatgccgcgt ccaagctgtt taaagcgggc
      361 ttccgttcga acaacatcga cccgaacgcg cgtcactgta tggcgtcggc agtagttggc
      421 tttatgcgta cctttggtat ggatgagccg atgggctgct atgacgacat cgagcaggct
      481 gacgcgtttg tgctgtgggg cgcaaacatg gcggagatgc acccgatcct ctggtcgcgc
      541 atcactaacc gtcgtctctc taatcagaac gtcaccgtgg cggtgctttc tacctaccag
      601 catcgtagct tcgagctggc ggataacggc atcatcttta cgccacagtc tgacctggtg
      661 atcctgaact acatcgccaa ctatatcatt caaaacaatg cgataaatca ggacttcttc
      721 agcaaacacg ttaacctgcg caaaggggcg acggacatcg gctacggttt acgtccgacc
      781 catccgctgg aaaaagcagc gaagaatcca ggttctgacg cctccgaacc gatgagcttt
      841 gaagattaca aagccttcgt tgccgagtat acgctggaaa aaaccgctga aatgaccggc
      901 gtgccgaaag accagttaga acaactggcg cagctgtatg ccgatccgaa caagaaagtc
      961 atctcctact ggacgatggg cttcaaccag catactcgtg gcgtgtgggc taacaacctg
     1021 gtctacaacc tgcacctgct gaccggcaaa atttcccagc cgggttgcgg tccgttctcc
     1081 ctgaccgggc aaccttctgc gtgtggtact gcgcgtgaag tgggcacctt tgctcaccgt
     1141 ctgcctgcgg acatggtggt gaccaacgag aaacaccgtg atatctgcga gaagaagtgg
     1201 aatatcccga gcggcaccat tccggcgaaa atcggtctgc acgcggtggc acaagaccgt
     1261 gcgctgaaag acggcaaact gaatgtttac tggaccatgt gtaccaacaa catgcaggcc
     1321 gggccgaaca tcaatgaaga gcgtatgccg ggctggcgcg atccgcgcaa cttcatcatc
     1381 gtctccgatc cgtatccgac agtcagtgcg ctggccgccg acttgatcct gccgaccgca
     1441 atgtgggtag agaaagaggg tgcttacggt aacgccgaac gccgtactca gttctggcgt
     1501 cagcaggtac aggcaccggg cgaagcgaaa tcggatctct ggcagttagt ccagttctcc
     1561 cgccgcttca aaactgaaga agtgtggccg gaagagctgc tagcgaagaa accggaactg
     1621 cgtggcaaaa cgctgtacga agtgctttat gccacaccgg aagtgagcaa attcccggta
     1681 tccgaactgg cggaagatca gctgaacgat gaatcccgcg agctgggctt ctatctgcaa
     1741 aaagggctgt tcgaagagta cgcatggttt ggtcgcggtc acggtcacga tctcgcgccg
     1801 ttcgatgact accacaaagc gcgcggtctg cgctggccgg tggtgaacgg taaagaaacg
     1861 cagtggcgtt acagcgaagg taacgacccg tacgtgaaag cgggcgaagg ctataagttc
     1921 tacggtaaac cggatggtaa agcggtgatc ttcgcgctgc cgttcgaacc ggcggcggaa
     1981 gcaccggatg aagagtacga cctgtggctc tctaccggac gcgttctgga gcactggcac
     2041 actggcagta tgactcgccg tgtgccggaa ctgcaccgcg ccttcccgga agcggtcttg
     2101 tttattcacc cgctggatgc gaaagcgcgc gatctgcgcc gtggcgacaa agtgaaagtg
     2161 gtttctcgcc gtggcgaagt gatctcgatt gttgaaacgc gcggtcgtaa ccgtccgcca
     2221 cagggcctgg tgtacatgcc gttcttcgac gccgcacagc tggttaacaa actaacgctg
     2281 gacgcgaccg atccgctctc gaaagagacg gacttcaaga agtgcgcagt caaactggag
     2341 aaggtgtaag ccattatgtc ccggtcagcg aaacctcaaa atggtcgccg ccgctttctg
     2401 cgcgatgttg ttcgcacagc aggcgggctg gctgccgtgg gtgtggcgct ggggttacaa
     2461 cagcaaaccg cacgcgcatc tggcgtgcgg ttgcgcccgc ccggagccat aaacgagaac
     2521 gcctttgcca gtgcctgtgt gcgttgcggt cagtgtgttc aggcttgccc ttacgacacc
     2581 ttaaaactgg cgacgctggc ttctggcctg tcggcgggca cgccatattt tgtcgcacgg
     2641 gatattcctt gcgaaatgtg tgaggacatt ccgtgcgcca aagtgtgccc gagtggtgcg
     2701 ctggatcgtg agatcgaatc gatcgacgac gcgcggatgg ggctggcggt gctggtggac
     2761 caggaaaact gtctcaactt tcaggggctg cgctgcgatg tttgttatcg cgaatgcccg
     2821 aaaattgatg aggccatcac cctggagctg gagcgcaaca cgcgtaccgg taagcacgcc
     2881 cgctttctgc cgacggttca cagcgacgcc tgtaccggct gcggtaagtg cgaaaaagtg
     2941 tgcgtgctgg aacaaccggc aatcaaggtg ttaccgctgt cactggcgaa aggggagtta
     3001 ggtcaccatt accgcttcgg ctggctggag gggaacaatg gcaaatcgta aacgtgacgc
     3061 cgggcgcgag gcgctggtga aaaaaggctg gtggcgcagt caccgttggc tggtgttacg
     3121 tcgcctttgt cagttcttcg tgctggggat gtttttgagc ggtccgtggt ttggtgtgtg
     3181 gatcttacac ggcaactaca gctgtagcct gttattcgac accgttccgc tgaccgatcc
     3241 gctgatgacg ctgcaaagtc ttgccagtgg tcatctgcct gccacggtgg cgttgaccgg
     3301 tgcggtgata atcactgtgc tctacgccct ggcaggaaag cgattatttt gcagctgggt
     3361 ctgcccgctg aacccgatta ccgacttagc gaactggtta cgcaggcgct ttgacctcaa
     3421 tcagtctgcg acgatccctc gccacattcg ctacgtgctg ttggtggtca ttctgctggg
     3481 atcagcctta accggcacgc tcatctggga atggatcaac cccgtttccc tgatggggcg
     3541 tagcctggta atgggcttcg gcagcggcgc gctgcttatt ctcgcgctgt ttttatttga
     3601 tctactggtc gttgaacacg gctggtgcgg gcacatttgt ccggttggcg cgctgtatgg
     3661 cgtgctgggt agcaaaggtg tgattaccgt tgcggccagc gatcggcaga aatgtaaccg
     3721 ctgtatggat tgttttcatg tttgcccgga accgcatgtg ctacgtgccc cggtgctgga
     3781 tgaacaaagc ccggtgcagg tcaccagccg cgattgcatg acgtgcggtc gctgcgtgga
     3841 tgtctgttct gaggatgtat ttacaataac tacacgatgg agttcgggag cgaaatcatg
     3901 aaaagccatg acctgaagaa agcgctgtgt caatggacgg cgatgctggc cctggtggta
     3961 agcggcgcgg tttgggcggc taatggcgtc gattttagcc agtcgccgga agtatccggg
     4021 acgcaggaag gggccattcg gatgccaaaa gagcaggatc ggatgccgct gaactatgtg
     4081 aatcagccgc caatgatccc gcatagcgtt gaaggttatc aggtaacgac caataccaac
     4141 cgctgcttgc agtgccacgg tgtcgaaagc tatcgcacca ctggcgcgcc gcgtatcagt
     4201 cctactcact ttatggacag cgacggcaaa gtgggcgcgg aagtggctcc gcgtcgttat
     4261 ttctgtctgc aatgtcacgt accgcaggcc gataccgcgc caatcgtggg gaataccttt
     4321 accccatcaa aaggttacgg gaaataagag gttattatgg gaaattctga ccgtaagcct
     4381 ggtctgatta agcgcctgtg gaaatggtgg cgtaccccca gccgtctggc gctgggaacg
     4441 ctgctgttga tcggttttgt cggcggcatc gtcttctggg gcggctttaa caccggaatg
     4501 gaaaaagcca ataccgaaga gttctgcatt agctgccacg aaatgcgtaa cacggtgtat
     4561 caggaataca tggattccgt gcactacaac aaccgtagcg gcgtccgtgc gacctgtccg
     4621 gattgtcacg ttccgcacga gtttgtgccg aagatgatac gcaagctcaa agcaagtaaa
     4681 gagctgtatg gtaaaatttt tggcgttatt gacacgccgc agaaatttga agctcatcgt
     4741 ctgacaatgg cacagaatga gtggcggcgc atgaaggaca ataactcgca ggagtgccgt
     4801 aactgtcaca acttcgagta tatggataca accgcccaga aatcggttgc cgcgaagatg
     4861 catgaccagg cggtgaaaga tgggcaaacc tgtattgatt gccataaagg gatagcgcac
     4921 aagctgcccg atatgcgtga agtcgagcca ggtttttaac agggttattg cgtgggtatg
     4981 cttgaagcca gagagttact ttgtgagcgg gatgaacgaa ccttatttag tggcttgtca
     5041 tttacgctga acgcaggaga gtgggtacaa atcaccggta gcaacggcgc ggggaagaca
     5101 acgcttctcc gtttgctgac ggggttgtct cgccctgacg caggcgaggt tctctggcaa
     5161 gggcagccct tgcatcaggt acgcgacagc taccatcaaa acctgttatg gataggccat
     5221 cagccgggga tcaaaacccg gctgacggcg ttagaaaatc tgcactttta tcatcgcgat
     5281 ggcgataccg cacaatgtct ggaagccctg gcgcaggccg ggcttgccgg attcgacgat
     5341 attccggtaa atcaactttc tgccgggcaa caacgccgcg tcgctttagc gcgtctgtgg
     5401 ctgacccgtg ccacgttatg gatcctcgac gagcctttta ccgcgattga cgttaacggc
     5461 gtcgatcgtc tgacccagcg tatggcgcag catacggagc agggggggat tgtgattctg
     5521 actacccacc agccgctcaa cgttgctgaa agtaaaattc gccgcatttc actgacgcaa
     5581 acgagggccg catgatgttc tggcgcattt tccgtcttga gctgcgtgta gcgtttcgcc
     5641 atagcgccga aatcgccaac ccgctgtggt tcttcctgat tgtaattacc ctttttccgc
     5701 tcagtatcgg tccggagccg caactgctgg cgcgtattgc accgggcatt atctgggttg
     5761 ctgcgctgct ttcatccttg ctggcgctgg aacgactgtt ccgtgacgat ttgcaggacg
     5821 gcagtcttga acaattgatg ttgttgccgt tacccttgcc cgccgttgtg ctggcgaagg
     5881 tgatggcgca ctggatggta accggtctgc cgttactcat cctttcgcca ctggtagcaa
     5941 tgctactggg aatggatgtt tatggctggc aagtgatggc gctgacgctg ctgctgggaa
     6001 cgcctacgct tggctttctc ggtgcaccgg gtgtggcgtt gacagtggga cttaagcgcg
     6061 gtggtgtgct gctcagcata ctggtgttac cgctgactat cccattactc atctttgcca
     6121 ccgccgcgat ggacgcggct tccatgcatt tgcccgttga cgggtatctg gcaattttag
     6181 gcgcgttgct ggcaggcacc gcgacattaa gtccttttgc gacggcggca gcgttacgaa
     6241 tcagcattca ataacgctgg attactgatt tttgttcgag tctggtatcg aaactatgtg
     6301 gaaaacactg catcaactgg cgatcccacc acggctgtat caaatctgtg gctggtttat
     6361 accgtggctg gcaattgcca gtgtggtcgt gcttaccgtc ggctggatct ggggattcgg
     6421 ctttgctccg gctgattatc agcagggaaa tagctaccgc attatctacc tgcatgtgcc
     6481 tgcggcgatc tggtcgatgg gcatttatgc atcaatggca gtggcagcgt ttattggcct
     6541 tgtctggcag atgaaaatgg ccaacctggc ggtggcggcg atggccccca ttggtgccgt
     6601 gtttaccttt attgccctgg ttaccggctc tgcatgggga aaaccgatgt ggggcacctg
     6661 gtgggtatgg gatgcacgtc tgacttctga actggtgctg ctgtttttgt atgtgggtgt
     6721 gattgccctg tggcacgcct tcgacgaccg ccgtctggcg ggccgtgcgg caggtatcct
     6781 ggtgctgatt ggcgtggtga atctgccgat tattcattac tccgtggagt ggtggaacac
     6841 cctgcatcag ggatcaacgc ggatgcagca aagtatcgat ccggcgatgc gttcgccgct
     6901 gcgctggtcg atttttggct tcctgctcct gtctgccacg ctgacgctga tgcggatgcg
     6961 taatttgatt ttgctgatgg aaaaacgccg tccgtgggtg agtgaactga tactgaaaag
     7021 aggccgtaaa tgacccctgc atttgcttcc tggaatgaat ttttcgcaat gggcggttac
     7081 gccttttttg tctggctggc ggtggtgatg accgttattc cgctggtggt tttggtcgtg
     7141 cactcggtga tgcaacatcg cgcaattctg cgtggtgtgg cgcaacagcg ggcgcgtgag
     7201 gcgcgtttac gtgctgcgca acagcaggag gctgcatgaa tattcgccgt aaaaaccgct
     7261 tgtggattgc ctgtgccgtg ttggcagggc tggcgctgac tatcggtctg gtgctatatg
     7321 cgctgcgctc gaatatcgat ctcttttata cgccggggga aattctctac ggcaagcgtg
     7381 aaactcagca aatgccggaa gtcggtcagc gtttgcgcgt tggcgggatg gtgatgctgg
     7441 gtagtgtgca gcgcgatccc aattcgctga aagtgacctt cactatttac gatgctgaag
     7501 gctcagtgga tgtctcttac gaaggcattt tgccggatct gttccgtgaa gggcagggcg
     7561 ttgtggtgca gggcgagctg gaaaaaggca atcatatcct cgcgaaagaa gtgctggcga
     7621 aacacgacga aaactacacg ccgccagaag ttgagaaagc gatggaagct aaccaccgtc
     7681 gcccggcgag tgtttataag gacccagcat catgatgccg gaaattggaa acgggctgct
     7741 gtgtctggcg ttagggattg cgctgctgct gtccgtgtat ccgctatggg gcgtggcgcg
     7801 cggagatgcg cgcatgatcg cgtcttcccg cttgtttgcc tggctgctgt ttatgtctgt
     7861 ggctggcgca tttctggtgc tggtcaatgc tttcgtggtc aacgacttta ccgtcaccta
     7921 tgttgccagc tactccaata cccagcttcc ggtgtggtat cgcgtggcgg ctacctgggg
     7981 cgcgcatgaa ggctcgctac tgctgtgggt gctgctgatg agcggctgga cctttgcggt
     8041 ggcgattttt agtcagcgta ttccgctgga tattgtggcc cgcgtactgg cgataatggg
     8101 gatggtcagc gtcggctttt tgctgttcat tctctttacc tctaacccat tctcacgcac
     8161 gctgccgaac ttcccgattg aagggcgcga tcttaacccg ctgttacagg acccggggct
     8221 gatcttccat ccgcctctgc tttatatggg gtacgtgggt ttctcggtgg cgtttgcttt
     8281 tgccattgct tctttgctga gcgggcgtct ggacagcact tatgcgcgtt ttactcgccc
     8341 gtggacgctg gcggcgtgga ttttcctgac gctcggcatt gtgctcggtt ccgcatgggc
     8401 ctattacgaa ctcggctggg ggggctggtg gttctgggat ccggtagaaa acgcctcgtt
     8461 tatgccgtgg ctggtgggga ctgcgctgat gcactcactg gcggtcactg aacaacgcgc
     8521 cagcttcaaa gcgtggacat tactgctggc aatcagtgcc ttctcgttgt gtctgttggg
     8581 gactttcctc gtgcgttccg gcgtgctggt atcggtacac gcgtttgcgt ctgatccggc
     8641 gcgcggtatg tttatcctcg cctttatggt gctggtgatt ggaggttcgc tgctgctgtt
     8701 tgccgcgcgt ggacacaaag ttcgctcacg cgtaaacaat gcgctgtggt cgcgggaatc
     8761 tctgctgtta gcgaacaacg ttttgctggt cgccgcgatg ctggtggtgt tgctggggac
     8821 gctgctgccg ctggtgcaca agcaactggg actgggcagt atttcgattg gcgaaccgtt
     8881 cttcaacacc atgtttacct ggctgatggt gccgtttgcg ctactgctgg gtgtcggtcc
     8941 tctggtgcgc tgggggcgcg atcgcccacg taaaatccgc aatttattga ttatcgcctt
     9001 catctcaacg ctggtgctgt ctctgctgtt gccgtggctg tttgagagca aagttgtggc
     9061 gatgacggtg ctcggcctgg caatggcctg ctggattgcg gtgctggcaa ttgcggaagc
     9121 tgcgctgcgt atttcacgcg gcacgaaaac caccttcagt tattggggga tggtggctgc
     9181 tcacctgggg ctggcagtga caattgttgg cattgctttt agccagaact atagcgttga
     9241 gcgtgatgtg cgcatgaagt ccggcgatag catcgatatt catgaatatc gcttcacctt
     9301 ccgtgatgtc aaagaggtga ctggcccgaa ctggcgtggc ggtgtggcga ctatcggcgt
     9361 aacgcgagag ggtaaaccgg aaacagtgct gtatgcggaa aaacgttatt acaacactgc
     9421 cgggtcgatg atgaccgaag cggcgattga cggtggcatc acgcgtgacc tgtacgccgc
     9481 gctcggtgaa gagctggaaa acggcgcgtg ggctgtgcgt ctttactaca aaccatttgt
     9541 tcgctggatt tgggcgggcg ggctgatgat ggcgttgggc ggactgctgt gtctgtttga
     9601 tcctcgctat cgtaagcgcg tgagtccgca aaaaactgcg ccggaggctg tatgaagcgc
     9661 aaagtattgt taattccgtt gattatcttc ctggcgattg ccgcggcgct gctgtggcag
     9721 ctggcgcgta atgccgaagg ggatgatccg accaatctgg aatcggcgct cattggcaag
     9781 ccagtgccga agtttcgcct cgaatcactg gacaatccgg ggcagtttta tcaggcggat
     9841 gtgctgactc agggcaaacc agtgctgctt aacgtctggg cgacctggtg tccgacctgc
     9901 cgtgcggaac atcaatatct gaatcagctt tctgcgcagg gcatccgcgt agtcggcatg
     9961 aactataaag acgatcgcca gaaggcaatc agctggctga aagagctggg caatccttac
    10021 gcgctaagcc tgtttgatgg cgatggcatg ttagggctgg atctcggtgt ctatggcgcg
    10081 ccagaaacgt tccttattga cggcaacggc atcattcgct atcgccatgc gggcgatctc
    10141 aatcctcgcg tctgggaaga agagatcaag ccgctgtggg agaaatacag taaggaggcc
    10201 gcacaatgag gtttttattg ggcatgctga tgctgatgat ctccggctca gcgctggcga
    10261 ccatagacgt gttgcagttt aaagatgaag cacaggaaca acagttccgt cagctcactg
    10321 aagaactgcg ctgcccgaaa tgccagaaca acagcattgc cgattccaac tcgatgattg
    10381 ccaccgacct gcgccagaaa gtgtatgaac tgatgcagga aggtaaaagt aaaaaagaga
    10441 ttgtcgatta tatggtggcg cgttacggca acttcgtcac ttacgatccg ccgttaacgc
    10501 cgctgaccgt gctgctgtgg gtgctgccag tagtagctat tggcattggc ggttgggtca
    10561 tatacgcccg ctcgcggcgt cgggtacgcg tggtgccgga agcgtttcct gaacaaagcg
    10621 tgccggaagg taagcgtgcc ggatatgtcg cttatttgcc gggcattgtg gtggcgttaa
    10681 ttgtggctgg cgtcagctac taccagaccg gaaattatca acaggtgaaa atctggcagc
    10741 aggccacggc acaggctccg gcgttgctgg acagggcgct ggatccgaaa gctgatccgc
    10801 tcaacgaaga agagatgtcg cgccttgcgc tggggatgcg tactcaactg caaaaaaatc
    10861 cgggagatat agacggctgg attatgttgg gccgcgttgg catggcgctg ggtaacgcca
    10921 gtatcgccac cgatgcatac gctactgcgt atcgcctcga tccgaaaaac agtgatgccg
    10981 cactgggtta tgctgaagcg ttgacacgtt catctgatcc caacgacaac cgcctcggcg
    11041 gcgaactgct acgtcagctg gtgagaacgg accatagcaa tatccgtgtg ctaagcatgt
    11101 atgcgtttaa tgcctttgag cagcagcgat ttggcgaagc cgttgccgcg tgggagatga
    11161 tgttgaaact cttacctgcc aacgatactc gccgtgcggt gattgaacgt agtatcgcgc
    11221 aggcgatgca acatttgtcg ccgcaggaga gtaaataaaa agaggcccga tggtgatgcc
    11281 atcgggctat ttttattgtg ccccgcgttg ttgcaggaac agaatggtgg ccgccacgcg
    11341 tgagcggaca ttgagtttac gcagcagatt tcgaatatga acttttactg tctgctcgga
    11401 aatattcaac acagaggcaa tctgtttatt tgacagcccc tgtgccagct cgtgcagaac
    11461 atccagctcg cgctccgtca gcacgctgaa gggatcttct tccgcgccaa acatttcacg
    11521 ttcacgtaag tactgattga cgcgttcgct aaagactttg ctgcctttcg ctccggcacg
    11581 aatcgcttcc agcaacactt ccgggtcgct gtctttcagc agataaccgt ctgcgcctgc
    11641 gtctatcagc gcaaagacat cgctggaggc atcggatacg gtcaggataa taatttgcgc
    11701 ggtaacgcca tccctgcgca aggcattgag ggtatccagg ccactcatac ctttcatatt
    11761 gagatccagc aagatcacgt cgatatccag tctattcgcc agatcgatcg cgctcgcgcc
    11821 gtcgcccgct tcggcgacca cttcaaagcc aggatcaagc tccagtaact gacgaacacc
    11881 gcgtcgcata agtggatgat catccacaat catcacctga aaaggcgttg cttcaggcat
    11941 agtagtctcc tgaggtttta ttagaatcat tatgtttgag cgtagcgttg gtaacgctac
    12001 caggcgcgtg aatgttaccc cattttatgt atggacatgt agtgaaaact acaaccttgc
    12061 cgttcgcttt ggcatcattt tcattataaa gacggaattt tgcatcagct tctcatcaag
    12121 aaaaacaact aaaaaatcaa tcacaatact tgtgcatttc tttaaacagt ttttaaaaac
    12181 tgactccggg tatggagcta tgggtatttt ctgtacccaa tgcttttaac agcaattaat
    12241 ttcataggat gaaagctcaa tgcatcaatc tggttctgtt tctctttgtc gttccgcaat
    12301 atctgttctg gtggctacag cgttatattc acccatagca ttggcatcaa cagttgagta
    12361 tggtgagaca gttgatggtg ttggcctgga aaaagatatc cagctggttt atgggaccgc
    12421 caataatacg aaaatcaatc ctggcggaga acagcatata aaagaatttg gtgtaagtag
    12481 taatactgaa attaacggcg ggtatcagta cattgaaatg aatggcactg cagaatactc
    12541 agtattaaat gatggttatc aaattgttca aatgggtggc gcggtaaacc agactacgct
    12601 caataatgga gtgctacagg tttatgg
//
LOCUS       DAJTZL010000121        12487 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.121, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000121 DAJTZL010000000
VERSION     DAJTZL010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12487
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.121"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            210..422
                     /gene="cspG"
                     /locus_tag="OO544_002927"
     CDS             210..422
                     /gene="cspG"
                     /locus_tag="OO544_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HCR6768467.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            433..621
                     /locus_tag="OO544_002928"
     CDS             433..621
                     /locus_tag="OO544_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCR6768468.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            596..826
                     /gene="ymcE"
                     /locus_tag="OO544_002929"
     CDS             596..826
                     /gene="ymcE"
                     /locus_tag="OO544_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HCR6768469.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGEM
                     AYNISYLTRVPGTNTLLACCCLLRPEEVNSEY"
     gene            816..989
                     /locus_tag="OO544_002930"
     CDS             816..989
                     /locus_tag="OO544_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HCR6768470.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(1038..2111)
                     /locus_tag="OO544_002931"
                     /pseudo
     CDS             complement(1038..2111)
                     /locus_tag="OO544_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415512.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
     gene            complement(2183..4927)
                     /gene="torS"
                     /locus_tag="OO544_002932"
     CDS             complement(2183..4927)
                     /gene="torS"
                     /locus_tag="OO544_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HCR6768471.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFAAQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     GFDTTAIEQQEQEISRSLRQQGELVGRRLQLRQQQQQLSQQIVAAADEIARLAQGQAN
                     NATTSAGATQAGIYDLIEQDQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPTLEKQLNNAVKILQRRQIRIEDPGVRAQVATTLTTVSQYSDLLALF
                     QQDSEISNHLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHQEKASARGQYSLL
                     LLGMVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     QLMGAFRSSVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKASQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATAIADDMPCALMGDPRRIRQVI
                     TNLLSNALRFTDEGYIILRSRTDGEQWLVEVEDSGCGIDPAKLAEIFQPFVQVSGKRG
                     GTGLGLTISSRLAQAMGGELSATSTPEVGSCFCLRLPLRVATAPVPKTVNQAVRLDGL
                     RLLLIEDNPLTQRITVEMLNTSGAQVVAVGNAAQALETLQNSEPFAAALVDFDLPDVD
                     GITLARQLAQQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLAHYL
                     QLQVNNDQSLDVSQLNEDAQLMGTEKIHEWLVLFTQHALPLLDEIDIARASQDSEKIK
                     RAAHQLKSSCLSLGMRIASQLCAQLEQQPLSAPLPHEEITRSVAALEAWLHKKDLNAI
                     "
     gene            5010..6038
                     /gene="torT"
                     /locus_tag="OO544_002933"
     CDS             5010..6038
                     /gene="torT"
                     /locus_tag="OO544_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HCR6768472.1"
                     /translation="MRVLLFLLLSLFMLPAFSADNLLRWHDAQHFTVQASMPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVASLPVIELVNAIDAPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(6011..6703)
                     /gene="torR"
                     /locus_tag="OO544_002934"
     CDS             complement(6011..6703)
                     /gene="torR"
                     /locus_tag="OO544_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HCR6768473.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            6823..7995
                     /gene="torC"
                     /locus_tag="OO544_002935"
     CDS             6823..7995
                     /gene="torC"
                     /locus_tag="OO544_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HCR6768474.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHARQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKDEK"
     gene            7995..10406
                     /gene="torA"
                     /locus_tag="OO544_002936"
                     /pseudo
     CDS             7995..10406
                     /gene="torA"
                     /locus_tag="OO544_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
     gene            complement(11045..11350)
                     /gene="cbpM"
                     /locus_tag="OO544_002937"
     CDS             complement(11045..11350)
                     /gene="cbpM"
                     /locus_tag="OO544_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HCR6768475.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(11350..12270)
                     /gene="cbpA"
                     /locus_tag="OO544_002938"
     CDS             complement(11350..12270)
                     /gene="cbpA"
                     /locus_tag="OO544_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HCR6768476.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNHQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGAKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
BASE COUNT         3011 a         3203 c         3269 g         3004 t
ORIGIN      
        1 ggacggctaa aataaaattt gcttaatctc aattatcatg cgttaatagc tgcgtcggtt
       61 tgaaagacag acagcataca aagtagttta ctaaagcagt tctcattatc aggcattatc
      121 cccttctttt gagtctctct cctgaacact aagtagtttc tgtattaaag ccctgtttgc
      181 cgaaaggccc aaaatgaagg aagtaaaata tgtctaataa aatgactggt ttagtaaaat
      241 ggtttaacgc agataaaggt tttggcttta tcactcctga tgatggcagc aaagacgttt
      301 tcgtccattt caccgccatc cagagcaatg aattccgcac gctgaacgaa aatcagaaag
      361 ttgaattttc tattgagcag gggcaacgtg gccccgcggc agcgaacgtt gttacgctct
      421 aaggttgccg ttattactca acatctccat ttccgctgtc catgttgtca tggttcacag
      481 taccgcacat cggcattcga tgtgacggag cgaaaccctt tgggcgctaa gtgtattttt
      541 tgtaaatcga cgatgatcac ctttgataac gtcgcgctgc aaatacgcac tgaccatgcg
      601 ccgctggatt tcacaaaata atatcaggct ccctcgtgga gcctttttta tatctgcctt
      661 atttttcttc aacgctgtat gtatagtaag cgataacctg ttgattattg aatctttcgg
      721 ggagatggct tataacattt cttacctgac cagggtaccg ggaaccaaca ccttactggc
      781 gtgttgctgt cttttaagac cagaagaggt taacagtgaa tattgaagag ttaaaaaaac
      841 aagccgaaac ggaaatcgcc gactttatcg cgcaaaaaat cgccgagctg aacaagaata
      901 cagggaaaga agtctctgaa attcgcttca ccgcacgaga aaaaatgacc gggcttgaaa
      961 gttatgatgt caaaatcaaa ataatgtgat tttgtgaaca tcaccccgtg cgaggtgatg
     1021 ttccgcttgt tgctaattta gtgaccaatc attggcgctt gtggaattaa gcgtcggtac
     1081 aattcctccg gcaccgggct ttgccatact cccgcataca ttgcgtaacc aatcaccgcg
     1141 aacataatcc tcagaaccag tagcgtcatt aaccagccag acaacgcaaa agctttttta
     1201 tttgccgcag gtttttgcag tgaaaaggtc aatgttgagg ctaccggaca tgactctacg
     1261 caagtcatac agccggtaca ttccactgtt cgtacctgaa ttaatttatc taccgggatc
     1321 cgtgatgggc aattttttgc gcatttgcca cagccgatac aactttcggc attgcgacga
     1381 atcttaaacg gcgataatag cgaaaccacg cccatcagcg cgccatatgg gcaaagataa
     1441 cgacactagg catggcgaat aaacaggctg gcaatcagca aaacggtcac gctgattaat
     1501 gtcgcggtcc ccatatgacg aaagaaatcg agcattttta cgtccatcac cacgctgtag
     1561 ggcgacaaca taaaatagtg aatcgcctga gcgggcatca ataacgcgat atagagaaaa
     1621 aaactcaaca gcaaatactt cacgccgcgc agaggaatat ccagccagcg gggaaggaca
     1681 cattgccgac caaacagttt gttaccgaga tcgccgatta attcagaaag cgtaccaacc
     1741 gggcataacc atgagcaaaa ggcctttttg agtaatagac tgatgacgat aaaagcgacc
     1801 aataacagca tcgcggcggt gtggacggac ggtaactgac ctgttgtaag gctatatttc
     1861 agattcatca gaccggcaat cggtagccag ccttcgatac ctcccggtct ggcgacaaat
     1921 gtcgtgctac ttgccgtttc gtaatagcgc acccaatacc agaacgtgat ggcaatataa
     1981 atattcattg ccaacagtaa taattgcgtc gctttacgcc aggtcgtggc attgcgccag
     2041 tcattccacg gtaatttgcc gcccgtcgtg cctggccgcc gctgccagcg ggttcttttt
     2101 ttctctgcca tgattttgcc agtccgttaa gttgcatacc aaatgccact attctagttg
     2161 ttcttaactg gctgatattg attcaaatcg cgttcaggtc tttcttatgc aaccatgctt
     2221 ccagagcggc aacactgcgt gtaatttctt cgtgtggaag gggggcagat aatggctgct
     2281 gctccagttg tgcgcatagc tggctggcga tacgcattcc caaactcaag caactgcttt
     2341 ttagctgatg cgcggcacgc tttattttct cgctatcctg actggcgcgg gcaatgtcga
     2401 tttcatcgag aagcggcagg gcatgttgtg taaataatac cagccattcg tggatcttct
     2461 ccgtccccat taactgagca tcttcattga gttgcgatac atccagcgat tgatcgttat
     2521 tgacttgcag ttggagatag tgcgccagta actgaccgag cacttcacgc ggcaccggtt
     2581 tagggataat cccgcggaat aatgaactgg tacgctggcg cagcgtttcg tcaatgacat
     2641 gggcgctaaa gccaatcaaa accagcgacg gatattgctg tgccagttgt cgggcaagcg
     2701 taatgccgtc gacatccggc agatcaaaat ccaccagtgc ggcagcaaac ggttcgctat
     2761 tttgcagtgt ctctaaagcc tgcgcggcat tgccaacagc aacaacctgc gcaccactgg
     2821 tgttcagcat ctcaacggta attcgctggg ttagcgggtt atcttcaatt aacagcaaac
     2881 gtaaaccgtc aagacgcacc gcctgattga ctgtttttgg cacgggtgcc gtggcaacac
     2941 gtaacggcaa gcgtaaacaa aaacagcttc caacctccgg cgtgctggtg gcgctcagtt
     3001 cgccgcccat cgcctgggcc agacggctac tgatagtcag ccccagcccg gtgccgccgc
     3061 gtttgccgct tacctgcaca aatggctgga agatttctgc cagtttcgcg ggatcaatac
     3121 cgcagccgct gtcttccact tcgaccagcc attgctcgcc atcagtgcga ctacgcagga
     3181 taatgtaccc ttcgtcagta aaacgcaggg cgttgctcaa caggttggtt ataacctgac
     3241 gaatacgtcg tggatcgccc attaacgcgc acggcatatc atcggcaatt gccgttgcca
     3301 ggcgaatcgg gcgacctttc acccgtccgc tcattaattg cagggtactt tccagcagcg
     3361 ggcgcggttc aaagggctca tcgctgaccg aaacattctt gccacctgct tcgatagcgg
     3421 aataatcgag aatatcgttg aggatggtca gcaacgattc accagagtca gtaattgccc
     3481 gcaaatcatc acgctgggcg ttaagtgcgg ggttatctgc cagtagctgt gcggtgccga
     3541 gaataccgta cagcggtgtg cggatctcat ggctcatcgc cgccagaaac gccgattttg
     3601 cctggctggc tttttctgct tccgcccgtg cctgtcggtg ttctatcact agttcctgca
     3661 attcagctgt acgcgctttg acctgcgccg ccagctgttc acggtggcga ttcagtgcat
     3721 gaacactgct gcgaaacgca cccatcagct gcccgatggt gtccagctcc cgtacgccag
     3781 cggtttccgg gaaaggggag tcaatatcac cgtccagcag ccgttgcagc gcctgcgttt
     3841 gttcggcaag tggacgcgtg actgagcgat aaaccacgcg ccagaggatc agaatcagtg
     3901 cgcaaagtga aaccatcccc agcaataaca ggctgtattg cccgcgtgca ctggcttttt
     3961 cctgatgcgc cagtccgtgc tgattacgca gctcaatggt gtcgaccagc tgactgactt
     4021 cgctactaaa ctgcgcgaac tgggcgatat tattttgggc gagagtttgc aggtgattgc
     4081 tgatttcact gtcctgctgg aacagcgcca acagatcgct atattggcta acggtagtta
     4141 acgttgttgc gacctgcgca cgaactcccg gatcttcaat gcgtatttgc cgacgttgca
     4201 gaattttcac cgcattattg agctgctttt ccagcgttgg tgcatttttc tggatctgct
     4261 ccagccccag attcatcacc atttgctgca cccgcagagc gctaaggcgc agttcattca
     4321 tctggttaac atactcaaga tcgatatcaa tcagccgatc gagtgcactt tcagcagcct
     4381 gacgctgatc ttgttcgatc aaatcgtaaa tcccggcctg ggtcgctcca gcggaagttg
     4441 tcgcattatt cgcctgacct tgcgccaggc gtgcgatctc atcggcggca gcgactatct
     4501 gctgactgag ttgctgttgt tgctggcgta gttgcagacg ccgccccacc agttcccctt
     4561 gctgacgtaa cgaacgggag atctcctgct cctgttgttc aatagcggtg gtatcaaaac
     4621 cttgttcccg taacgcttgc agcaacgcat taatcttcag gctttgtgcg gtgagcattc
     4681 gcccctgcgc ctgccacatc ttttcgttat cggcactggt caggttctgc gcggcgaaaa
     4741 gttcccaggc gctggcttcg ctcaactggc gcgccatatt catggtagga atcaatgcct
     4801 gagtattgtc tttttccacc tggctgataa agcgcaggtt gtaccatccc accagggtac
     4861 tggtcagggt taacagcgcc atcagggcaa agcccatcca gagtcttcgg gtcagggtta
     4921 aattcacggt cggtgcactt taggtgaaaa atgttgagtc gcaaagcgga atgcatctag
     4981 cataaagcct tattattgat gaggctatca tgcgcgtact gctattttta cttctttccc
     5041 ttttcatgtt gccggcattt tcggctgata acctgttgcg ctggcatgat gcgcagcatt
     5101 tcacggtgca agcctctatg ccgcttaaag ccaaacgcgc atggaaactg tgcgcgcttt
     5161 atcccagcct gaaagattca tactggttat cgttgaacta tggtatgcag gaggcagctc
     5221 gccgttatgg tgtggattta aaagtgctgg aggcaggcgg ctacagccag ttggctaccc
     5281 agcaagcaca aatcgaccag tgtaaacagt ggggcgcaga ggccatttta ctcggtagta
     5341 gcacgacctc atttcccgat ctgcaaaagc aggtagcaag tctgccggtg atcgaactgg
     5401 taaatgctat tgatgctccc caggtgaaaa gccgcgttgg tgtaccctgg tttcagatgg
     5461 gctatcaacc tgggcgatat ttggtgcaat gggcgcacgg taaaccactg aacgtgctgt
     5521 tgatgcccgg acccgataat gccgggggca gtaaagagat ggtcgagggt tttcgcgcag
     5581 ccattgccgg aagcccggtg cgtattgttg atattgcgct tggtgataac gatattgaaa
     5641 tccagcgtaa cctgttgcag gagatgctgg aacgccaccc agaaatcgac gtcgttgccg
     5701 gaacggccat tgcggcagag gcggcaatgg gtgaagggcg taacctgaaa acgccgctta
     5761 ccgtggtgtc gttttatctt tcacatcagg tgtatcgcgg gctgaagcgg ggaagagtga
     5821 ttatggctgc cagcgatcaa atggtctggc agggggaact ggcggttgag caggccatca
     5881 ggcaattaca ggggcaatcg gtgtctgata atgtcagccc accgatttta gttctgacgc
     5941 cgaaaaatgc cgaccgtgaa catattcgcc gctcgctgtc accaggggga tttcgtccgg
     6001 tctattttta tcagcacaca tcagcggcta agaaataacc ttcaccatgt tgcgtcacca
     6061 gtaaatccgc gctgagttta tgacgtaaac gacgaattaa cacatcgacg gtgcgcaggt
     6121 cagggttttc cacccgacgc gcagaaagca tacgtagcag acgttcacgg ctgagaattt
     6181 cgcccggatt cgtcacaaat gccaccaaca tttcatactc tgcgcgggtc agtttaatcg
     6241 gctcgccatc ccgctccagc gtatggcgcg acacattcag gcaataaccg gcaaagcgat
     6301 agcagttgtc ctgagtgtgc ggttgagctt gtcgcgcgag gtcgattcgc cagagcagat
     6361 ttttcacccg tactaccagt tcgcgcagtt ccagcggttt ggtgacgtaa tcgtctgcgc
     6421 ccatttccag cccaacaata cggtcaatcc gatcgctgcg tccggtaacc agaataatcc
     6481 ccaccgttga gcgttctcgc agggcgcggg ttaacatcag gccattttca tcgggtaagt
     6541 tgatatccag cagaattaaa tctaccggct gattctgcat aatttcccgc agcccggcac
     6601 cgctcgctgt aacggaaacg gtatacccct cctgagtgaa gtaagattgt aatcgcgcct
     6661 gggtaaccgg ctcatcttca acaataacaa tgtgatgtgg catcagaggg ttttactcat
     6721 tctgttcata ttctgccgta agccgttcat cctgaccagt gccgctgttc atatttgctc
     6781 attaagatcg cttcactaaa ccataatttt acaggggcta ttatgcggaa actctggaac
     6841 gcgctacgcc gacccagtgc tcgttggtcg gtactggcgc tggtcgctat tgggattgtg
     6901 attggcattg cgctgattgt attgccacac gttgggatca aagtcaccag cacaaccgaa
     6961 ttttgtgtca gttgccacag tatgcaaccg gtgtatgaag aatataaaca gtcggtgcat
     7021 tttcagaacg cctctggcgt gcgagctgaa tgccatgact gtcatatccc gccggatatt
     7081 ccaggcatgg tgaagcgcaa actggaagcg agcaacgaca tttaccagac ctttattgcc
     7141 cactccattg atacacctga aaaattcgaa gccaaacgcg cggaacttgc cgagcgtgaa
     7201 tgggcgcgaa tgaaagaaaa caactcggca acctgtcgct cctgccataa ctacgatgcg
     7261 atggatcatg ccaggcagca tcctgaagcg gcacgtcaga tgaaggtggc agcgaaagat
     7321 aatcaatcct gcatcgactg tcataaaggt attgcccacc agttaccgga tatgagtagc
     7381 ggcttccgta agcagttcga tgagctgcgc gccagtgcta atgacagtgg tgacacgctg
     7441 tactctattg atattaagcc aatttatgcg gcgaaaggcg ataaagaagc ctctggttct
     7501 ctgctgcctg cttcggaagt gaaagtcctt aaacgtgacg gcgactggct gcaaattgaa
     7561 attaccggct ggacggaaag cgccggacgt cagcgtgtac tcacccaatt cccaggtaaa
     7621 cgcatctttg ttgcctcgat tcgtggtgat gtgcagcagc aggtgaaaac gctggagaaa
     7681 accaccgttg ccgacaccaa taccgagtgg agcaagttgc aggccaccgc gtggatgaag
     7741 aaaggcgaca tggtgaacga tatcaaaccg atctgggcct atgcggattc gttgtacaac
     7801 ggcacctgta accagtgcca cggcgcaccg gaaatcgccc actttgacgc taacggttgg
     7861 atcggcacgc tcaacggtat gattggcttt accagcctcg ataaacgtga agaacgcacc
     7921 ttgttgaaat atctgcaaat gaatgcgtct gacaccgcag gtaaggctca cggcgataag
     7981 aaggacgaaa aataatgaac aataacgatc tctttcaggc atcacgtcgg cgttttctgg
     8041 cacaactcgg cggcttaacc gtcgccggga tgctggggcc gtcattgtta acgccgcgcc
     8101 gtgcgactgc ggcgcaagcg gcgactgagg ctgtcatctc gaaagagggc attcttaccg
     8161 ggtcgcactg gggggctatc cgcgcgacgg tgaaggatgg tcgctttgtg gcggcaaaac
     8221 cgttcgaact ggataaatat ccgtcgaaaa tgattgccgg attgccggat cacgtacaca
     8281 acgcggcgcg tattcgttat ccgatggtac gcgtggactg gctgcgtaag cgccatctca
     8341 gcgatacctc ccagcgcggt gataaccgtt ttgtgcgcgt gagctgggat gaagccctcg
     8401 acatgttcta tgaagaactg gaacgcgtac agaaaactca cgggccgagt gccttgctga
     8461 ccgccagtgg ttggcaatcg acggggatgt tccataacgc ttcggggatg ctggcgaaag
     8521 ctattgcctt gcatggtaat agcgttggta cgggcggaga ttactctacc ggtgctgcgc
     8581 aggtgatcct gccgcgcgtg gttggctcaa tggaagtgta tgaacagcaa acctcctggc
     8641 cgctggtatt gcagaacagc aaaaccattg tgctgtgggg ctctgatttg ctgaaaaacc
     8701 agcaagcgaa ctggtggtgc ccggatcacg atgtttatga atattacgcg cagctaaaag
     8761 cgaaagtcgc cgccggtgaa attgaggtca tcagcatcga tccggttgtc acatccaccc
     8821 atgagtatct ggggcgcgag catgtgaagc acattgcggt taacccgcaa actgatgtgc
     8881 cgctgcaact ggcgctggcg tatacgctgt acagtgaaaa cctgtacgac aaaaacttcc
     8941 tcgctaacta ctgtgtgggt tttgagcagt tcctgccgta cctgctgggt gagaaagacg
     9001 gtcagccgaa agatgccgca taggctgaaa aactgaccgg cattgatgcc gaaaccattc
     9061 gtgggctggc gcggcagatg gcggcgaaca gaacgcaaat tattgctggc tggtgcgtgc
     9121 agcgtatgca gcacggtgaa cagtgggcgt ggatgattgt ggttctggcg gcgatgctgg
     9181 ggcaaattgg cctgccaggt ggtggctttg gttttggctg gcactataac ggcgcaggca
     9241 cgccggggcg taaaggcgtt attctgagtg gtttctccgg ctctacgtcg attccgcctg
     9301 ttcacgacaa cagtgattac aaaggctaca gcagcaccat tccgattgcc cgttttatcg
     9361 atgcgatcct cgaaccgggg aaagtgatca actggaacgg taaatcggta aaactgccgc
     9421 cgctgaaaat gtgtattttt gctggaacta acccattcca tcgccatcag cagatcaacc
     9481 gcattattga aggctggcgc aagctggaaa cggttatcgc catagataac cagtggacct
     9541 caacctgccg ctttgccgat atcgtgctgc ctgcgaccac gcagtttgag cgtaacgatc
     9601 tcgaccagta cggcaaccac tccaaccgtg gcattatcgc catgaaacag gtggtgccgc
     9661 cgcagttcga ggcgcgcaac gactttgata ttttccgcga gctgtgccgt cgctttaatc
     9721 gcgaagaagc ctttaccgaa gggctggacg aaatgggctg gctgaaacgc atctggcagg
     9781 aaggtgtaca acaaggcaaa ggacgcggcg ttcatctgcc agcgtttgat gacttctgga
     9841 ataacaaaga gtacgtcgag tttgaccatc cgcagatgtt tgttcgccac caggcattcc
     9901 gcgaagatcc ggatctcgaa ccgctgggca cgccgagtgg cctgattgag atctactcga
     9961 aaaccatcgc cgatatgaac tacgacgatt gtcaggggca tccgatgtgg tttgagaaaa
    10021 tcgaacgctc ccacggtggg cccggctcgc agaagtatcc gttgcatctg caatctgtgc
    10081 atccggattt ccgacttcac tcgcagttat gtgagtcgga aactctgcgt cagcaatata
    10141 cggtagcggg taaagagcca gtattcatta acccgcagga tgccagcgcg cgcggtattc
    10201 gtaacggtga tgtggtacgc gtctttaacg ctcgcggtca ggtgttggca ggggcagtgg
    10261 tttctgaccg ctatgcaccc ggcgtggcac gaattcacga aggggcatgg tacgatccag
    10321 ataaaggcgg cgagcctggt gcgctgtgca aatacggtaa ccccaacgtg ttgaccatcg
    10381 acatcggtac atcgcagctg gcgcagttgt tctcccgtga actggacgat gaacaactga
    10441 cgcaaatcgc cagtgcgcag atggctgaat ggttttcgtt gctgaaaagc gaaccgccgc
    10501 tcactgcggc ggtgaatgcg ctggaaaacc gtattgccgc tctgacagta cgtgacgatg
    10561 cccgtctgga actggccgct gacttttgcg gcctgtttct gatgaccgac aaacaagcgg
    10621 cgctgccgta tgcatcggcc tacaaacagg acgagcaaga gattaaacgc ttgttagttg
    10681 aggcagggat ggaaaccagt ggcaatttca acgaatcggc agatcatctg gcgatctatc
    10741 tcgagttgtt gagtcatctg catttttcgc tgggagaggg gaccgttcct gcgcgaagaa
    10801 tcgacagttt gcggcaaaaa acactgacgg cgctgcggca atggttacca gagtttgctg
    10861 cgcgttgtcg acagtatgac agctttggtt tttacgcggc actaagccag ttattgctgg
    10921 tgttagtgga gtgcgaccac caaaacagat aacgtcgttt atgcgcctga aaagacgcgt
    10981 ttagcgtcgc atcaggcatt atggcgcagt tgccggatgc gtcatcacat ccggcaaaca
    11041 cacatcacgg atgagctaca aaccgggaaa gccgctggcg tagcaggcgg ttttcctgct
    11101 tcaggtgcgc aatatcatcc attaacgtca gcgccaccgc gatccccggc cagtccagag
    11161 ccagttcatg acgcaggcgt accgcgcgtt gcaccacaat ggcggcatgg tcgtcaaata
    11221 cccaggttgt ttcctggatc tcacgcggtt caaccacccc caaaccgaca atttcattca
    11281 actcctcttc agagatgccg gtatgcaggc aaaattcggt aatagtaaaa gtcaccgtaa
    11341 cattagccat catgctttcc cccaatcttt acgtggatca aaagacgact gggcgtctgc
    11401 cagttgctgc cacagcgcgg cagtgttttc atccggtttc ggcggcatca cgattttcag
    11461 taccgcatac agatcgccgg tctgtttttt gctcaccaga cctttgcctt taacgcgcaa
    11521 tcgttgcccg gcctggctgc ctggcgggat agtcagcaaa atgctttctt tcagtgttgg
    11581 aacggtgact ttagcaccca gcgccgcttc ccacgggcta accggcacca caatttccag
    11641 atcctggccg acaatatcga acagcggatg tggcgcaata tgaatcacca gccacaaatc
    11701 gccatttgga ccaccgtttt cgcccggcgt cccctggcct ttcagacgga tgcgttgacc
    11761 attgccgacg cccgccggga tcttcacatt cagcgttttc gggatttcct gttcgatcat
    11821 gccaaaggcg ttataaaccg gcaggttata gctgatggta cgcttatgct cagtcagcgt
    11881 ttcttcgagg aataccgcca cttcgatttc aatatcgtgg ccgcgtgcgg cggggcgttg
    11941 acggctctgg cgggcatgct gaccgaaaat tgacgagaag atatcgtcaa aatcttcggc
    12001 attaaaactc tgaccgtcgc catgatggaa ctgatggtta aattgcggat cgttgcgatg
    12061 ttgccacatc tgatcatact cagcgcgacg ttgttcatca cttaacactt cccaggcttc
    12121 agcgacctct ttgaagcggg cttcggcatc cggttctttg ctgacatcag gatggtattt
    12181 acgggccagt cgacgatagg cggtcttgat tgtcttgaga tcgtccgtcg gtttcacgcc
    12241 catgatggcg taataatcct ttaattccat agcgttatct cgcgtaaatc aacacaaatt
    12301 gaaggaaccc ctgtaaggta actcctataa gtgtagggta atcctcaaaa tttcatatgc
    12361 caacacagaa tatgttattg aaatcatcgc ggagaggagg tcgccatcaa gatgggttgc
    12421 tgaacatatt ttaaacaggt gaaaaagggt gagcgatttt tgatagttga accaggcact
    12481 ttaagtt
//
LOCUS       DAJTZL010000122        12447 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.122, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000122 DAJTZL010000000
VERSION     DAJTZL010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12447
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.122"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..191
                     /gene="bdm"
                     /locus_tag="OO544_002939"
     CDS             <1..191
                     /gene="bdm"
                     /locus_tag="OO544_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HCR6768477.1"
                     /translation="STAEPALVNAIEQGLRAEQGVVTEDDILMELTKWVEASDNDILS
                     DIYQQTINYVVSGQHPTL"
     gene            293..430
                     /gene="sra"
                     /locus_tag="OO544_002940"
     CDS             293..430
                     /gene="sra"
                     /locus_tag="OO544_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HCR6768478.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            587..2284
                     /gene="maeA"
                     /locus_tag="OO544_002941"
     CDS             587..2284
                     /gene="maeA"
                     /locus_tag="OO544_002941"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HCR6768479.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            2418..3420
                     /gene="adhP"
                     /locus_tag="OO544_002942"
                     /pseudo
     CDS             2418..3420
                     /gene="adhP"
                     /locus_tag="OO544_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415995.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
     gene            3566..3850
                     /locus_tag="OO544_002943"
     CDS             3566..3850
                     /locus_tag="OO544_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HCR6768480.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVTQ"
     gene            complement(4078..4731)
                     /gene="fdnI"
                     /locus_tag="OO544_002944"
     CDS             complement(4078..4731)
                     /gene="fdnI"
                     /locus_tag="OO544_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HCR6768481.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(4724..5608)
                     /gene="fdnH"
                     /locus_tag="OO544_002945"
     CDS             complement(4724..5608)
                     /gene="fdnH"
                     /locus_tag="OO544_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HCR6768482.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEERVAKLKARGYEHAGVYNPKG
                     VGGTHVMYVLHHADQPELYYGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(5621..8668)
                     /gene="fdnG"
                     /locus_tag="OO544_002946"
     CDS             complement(5621..8668)
                     /gene="fdnG"
                     /locus_tag="OO544_002946"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(8081..8083),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HCR6768483.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            8900..9781
                     /gene="yddG"
                     /locus_tag="OO544_002947"
     CDS             8900..9781
                     /gene="yddG"
                     /locus_tag="OO544_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HCR6768484.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGACLWIYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
     gene            10040..11140
                     /gene="ompC"
                     /locus_tag="OO544_002948"
     CDS             10040..11140
                     /gene="ompC"
                     /locus_tag="OO544_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HCR6768485.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGNKTRLAYAG
                     LKISEFGSLDYGRNYGVGYDIGSWTDMLPEFGGDTWSQKDVFMTYRTTGLATYRNYDF
                     FGLIEGLNFAAQYQGKNERTDNSHLYGADYTRANGDGFGISSTYVYDGFGIGAVYTKS
                     DRTNAQERAAANPLNASGKNAELWATGIKYDANNIYFAANYAETLNMTTYGDGYISNK
                     AQSFEVVAQYQFDFGLRPSLAYLKSKGRDLGRYGDQDMIEYIDVGATYFFNKNMSTYV
                     DYKINLIDESDFTRAVDIRTDNIVATGITYQF"
     gene            11304..11786
                     /locus_tag="OO544_002949"
                     /pseudo
     CDS             11304..11786
                     /locus_tag="OO544_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707621.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            11901..>12447
                     /locus_tag="OO544_002950"
     CDS             11901..>12447
                     /locus_tag="OO544_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HCR6768486.1"
                     /translation="MITDLILHNHPRMKTITLNDNHIAHLNAKNTTKLEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNGINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFDATNYDRLVKLSLNSNALESINFPQGRNVSITHISMN
                     NNALRNIDIDRLSSVTYFSAAH"
BASE COUNT         3229 a         3104 c         2968 g         3146 t
ORIGIN      
        1 attcaacagc agaacccgct ctggttaatg ccattgagca aggccttcgg gcagagcaag
       61 gtgttgtcac tgaagatgac attctcatgg agctgaccaa atgggtggaa gcttctgata
      121 acgacatcct cagtgatatc taccagcaaa cgatcaatta tgtggtcagt ggccagcacc
      181 ctacgcttta aggtgctatg cttgatcggc aacctaattt aggggtttag cacgtgtttc
      241 ttcgctacgg cgatgttgtc cttaaaacta gcaacaggat tgaggagtta aaatgaaatc
      301 gaaccgtcag gcacgtcata ttcttggact ggaccataaa atttctaacc agcgcaaaat
      361 agttaccgaa ggtgacaaat ccagcgtggt aaataaccca accggcagaa aacgccccgc
      421 tgaaaagtaa ttcataacca tcagtcctca atgacgatta aacaccattg cctgcgcaat
      481 ggtgtttttg tttttatctg ctttatactt ggggccgacg ccctggcggt aaagcaaaga
      541 cgataaaagc gtgccaggga tggatattca aaaaagagtg agtgacatgg aaccaaaaac
      601 aaaaaaacag cgttcgcttt atatccctta cgctggccct gtactgctgg aatttccgtt
      661 gttgaataaa ggcagtgcct tcagcatgga agaacgccgt aacttcaacc tgctggggtt
      721 actgccggaa gtggtcgaaa ccatcgaaga acaagcggaa cgagcatgga tccagtatca
      781 gggattcaaa accgaaatcg acaaacacat ctacctgcgt aacatccagg acaccaacga
      841 aaccctcttc taccgtctgg taaacaatca tcttgatgag atgatgcctg ttatttatac
      901 cccaaccgtc ggcgcagcct gtgagcgttt ttctgagatc taccgccgtt cacgcggcgt
      961 gtttatctct taccagaacc gccacaatat ggacgatatt ctgcaaaacg taccgaacca
     1021 taatatcaaa gtgattgtgg tgactgacgg tgaacgtatt ctggggcttg gtgaccaggg
     1081 catcggcggg atgggcattc cgatcggtaa actgtcgctc tataccgcct gtggcggcat
     1141 cagcccggcg tatacccttc cggtggtgct ggatgtcgga acgaacaacc aacaactgct
     1201 taacgatccg ctgtatatgg gctggcgtaa tccgcgtatc actgacgacg aatactatga
     1261 attcgttgat gaatttatcc aggctgtgaa acaacgctgg ccagacgtgc tgttgcagtt
     1321 tgaagacttt gctcaaaaaa atgcgatgcc gttacttaac cgctatcgca atgaaatttg
     1381 ttcttttaac gatgacattc agggcactgc ggcggtaaca gtcggcacac tgatcgcagc
     1441 aagccgagcg gcaggtggtc agttaagcga gaaaaaaatc gtcttccttg gcgcaggttc
     1501 agcgggatgc ggcattgccg aaatgatcat cgcccagacc cagcgcgaag gattaagcga
     1561 ggaagcggcg cggcagaaag tctttatggt cgatcgcttt ggcctgctga cggacaagat
     1621 gccgaacctg ctgcctttcc agaccaaact ggtgcagaaa cgcgaaaacc tcagtgactg
     1681 ggataccgac agcgatgtgc tgtcactgct ggatgtggtg cgcaatgtaa aaccagatat
     1741 tctgattggc gtctcaggac agaccgggct gtttacggaa gagatcatcc gtgagatgca
     1801 taaacactgt ccgcgtccga tcgtgatgcc gctgtctaac ccgacgtcac gcgtggaagc
     1861 cacaccgcag gacattatcg cctggaccga aggtaacgcg ctggtcgcca cgggcagccc
     1921 gtttaatcca gtggtatgga aagataaaat ctaccctatc gcccagtgta acaacgcctt
     1981 tattttcccg ggcatcggcc tgggggttat tgcttccggc gcgtcacgta tcaccgatga
     2041 gatgctgatg tcggcaagtg aaacgcttgc acagtattcg ccgctggtgc tgaacggcga
     2101 aggtctggta ctaccggaac taaaagatat tcagaaagtc tcccgtgcaa ttgcgtttgc
     2161 ggttggcaaa atggcgcagc agcaaggcgt ggcggtgaaa acctctgccg aagctttgca
     2221 acaagccatt gacgataatt tctggcaagc cgaataccgc gactaccgcc gtacctccat
     2281 ctaagcctgc gcccggtagt gaaggctacc gggcaatttt cctctccctt tttcagatct
     2341 catccatact gggtagtggc gaataaatct catttgcctc acctgctatg cagaacatca
     2401 tccgaaaagg aggaactatg aaggctgcag ttgttacgaa ggatcatcat gttgacgtta
     2461 cggataaaac actgcgctca ctgaaacatg gcgaagccct gctgaaaatg gagtgttgtg
     2521 gtgtatgtca taccgatctt catgttaaga atggcgattt tggtgacaaa accgacgtaa
     2581 ttctgggcca tgaaggcatc ggtgtggtgg cagaagtggg tccaggtgtc acctcattaa
     2641 aaccaggcga tcgtgccagc gtggcgtggt tctacgaagg atgcggtcat tgcgaatact
     2701 gtaacagtgg taacgaaacg ctctgccgtt cagttaaaaa tgccggatac agcgttgatg
     2761 gcggaagagt gcatcgtggt cgccgattac gcggtaaaag tgccagatgg tctggactcg
     2821 gcggcggcca gcagcattac ctgtgcgggg gtcaccacct acaaagccgt taagctgtca
     2881 aaaattcgtc ccgggcagtg gattgctatc tacggtcttg gcggtctggg taacctcgcc
     2941 ctgcaatacg caaagaatgt ctttaacgcc aaagtgatcg ccattgatgt caatgatgag
     3001 cagttaaaac tggcaaccga aatgggtgca gatttagcga ttaactcacg caccgaagac
     3061 gccgccaaaa ttgtgcagga gaaaaccggt ggcgctcact ctgcggtggt aacagcagta
     3121 gctaaagctg cgtttaactc ggcagttgat gctgtccgtg caggcggtcg tgttgtggct
     3181 gtcggtctgc cgccggagtc tatgagcctg gatatcccac gtcttgtgct ggatggcatt
     3241 gaagtggtcg gttcgctggt cggcacgcgc caggatctaa ccgaagcctt ccagtttgcc
     3301 gccgaaggca aagtggtgcc gaaagtcgcc ctgcgtccgt tagcggacat caacaccatc
     3361 tttaccgaga tggaagaagg caaaatccgt ggccgtatgg tgattgattt ccgtcactaa
     3421 gaggtctttg ctgcgactcc catgttcggg tcgcagcatc gcacactctc caacatgaaa
     3481 tggctaaatg gattattgtt gcgtaggaag gaacaataat gatagtgttc ccagagaagg
     3541 aacgctactg agagtaaaga cactaatgaa aatggccaat catccccgcc cgggggacat
     3601 tattcaggaa tcactggacg aacttaatgt cagcctgcgc gagtttgcca gagcaatgga
     3661 aattgcgccc tcaacggcaa gccgattgct gaccggaaaa gcagctttga cgccagaaat
     3721 ggcaattaaa ctttccgtgg tgatcggcag ttcgccgcaa atgtggctga atctgcaaaa
     3781 cgcctggagt ctggcagagg cagaaaaaac ggtggatgtg tcgcgattac gccgtctggt
     3841 tacgcaataa aaaatggcac cttataaagc gccatttgaa actgatgaca aacgcaacat
     3901 tgcctgatgc gctacgctta tcaggcctac acgttcactt caatctattg tatttgaagg
     3961 gttttcatgc cggataaggc attcacgacg catccgggat gaacaaagcc cacgttgtct
     4021 acaatatgaa aatggcgcct tttacagcgc cattttcagc ttaaagttca aaagagatta
     4081 tatcccttct tcactctctt ttttcgcttc tgccttctcg atttcacgat accagcgcgg
     4141 atggtgtttc ttcgcccagc gacggctcac cttcccttcg atcatccctt taatcgaccc
     4201 tttcacccag aatgccatat acatatggat caggatggcg tggatcagga tgatacccgc
     4261 agccgcgtgg atcagcaggc tgtagcgaac aacctgcatc gggaagtact gcgcaaagta
     4321 cggacgccag ataatcaccc cggtcaccag cagcacgaaa atcatgctca tgatcgacca
     4381 gaacatcatc ttttgcccgg cgttgtactt accaacatcc gccactttgt gctcattgcc
     4441 ttttaatact tcgacaatgt tcaacagcca cggaatatct ttcttatccg ggatgttgtg
     4501 atgcacaaaa cgcacaaaca taaacatcag tgcgacgaaa atcgcaatgc cgaagaacgg
     4561 gtgcaaaatg cgtcccatct gcggcgtacc gaaggtttgc gtcagccatt gcagcgtcgg
     4621 gaagaagaac gaaatcccgg acagcgccac caggaagaag caaatcacca cggtccagtg
     4681 acaggcgcga tcaataaatt tggtgcgcac aatcattttc gacttactca tgatgatcct
     4741 cctcgtcatc gtccacttcc ttattcgggc caataccgat gtagtggaaa atcaacccgg
     4801 caaaagtggc aataaagcca gccgctgcca gcggtttcaa cgcgcctttc cacaggctta
     4861 ccgaggtgtc gatcttcgga tctttcggca gaccgtaata cagctccggc tgatcggcgt
     4921 gatgcagcac gtacataacg tgcgtaccac cgaccccttt cgggttgtag acgccagcat
     4981 gttcgtaacc acgcgctttc agtttcgcca cgcgctcttc cgccagctcc agcatctcct
     5041 tcttggtgcc gaagtggata gccccggtcg gacaggtttt cacacaagcc ggttcctggc
     5101 cgacgctgac gcgatcgacg cagagcgtgc atttatatac ccggttatcc tctttgttga
     5161 ggcgcggaat attaaacgga cacccggcaa tgcagtaacc acagccgatg cagttttccg
     5221 actggaaatc gacaatcccg ttagcgtact gaatgattgc accagcagac gggcacgcct
     5281 tcaggcagcc gggatcttca cagtgcatac agccgtcttt acggatcagc cactccagct
     5341 tgccgttctg ttcggtttcg ctaaagcgca tcaccgtcca ggacttggcg ctcagatcgg
     5401 cggggttatc gtaaaccccg acgcagtgcc ccacttcatc acggatgtcg ttccactccg
     5461 aacacgccac ctgacaggct ttacagccga tacaggtgga aacgtcgata agttttgcga
     5521 cttctgcttt gtaatcacgc acctgagaag gcggcgtgat ggagttagtt gcggaccttt
     5581 tgataatatc ctgcgtttcc atagccatct gttcgccccc ttacgccttc tcgatgttga
     5641 ctaagaacgc tttatattcc ggcgtttgcg agtttgcatc accgacattc ggcgtcagag
     5701 tgttagcgat ataaccttta cgcgcgacac cctcaaagcc ccagtggatt ggaataccca
     5761 ccgtttcaac ctgctgaccg tttacattca gcggtttcag acggcgcgtt accaccgcca
     5821 ccgcgcggat aaagccacgc ttgcttgaga cagtgacacg atcgccatta ttaatgcctt
     5881 tcgccgccgc cagcgtttcg ctgatttcca caaactgttc cggctgagca attgcgttga
     5941 gcaatgcgtg cttggtccag gtgtggaagt gctcggtcag acgataggtc gtacccacat
     6001 acgggaactg ctctttttta cccatccgca gcgcgtcttg ttcatacaga cgaacaaccg
     6061 ggttagacac cacgttcgga tgcagcgggt tagtgcccag cggcgtttca atcggctcgt
     6121 agtgttccgg gaacggacct tccgccattt tgttgatggc aaacaggcgt cccatccctt
     6181 ccggctgcat gataaacggc ccggttggcg tacccggtgc ggcattgccg aagtccggaa
     6241 tatcgttacc cgtccacttg ctgccgttcc actggatcag catccgtttc ggatcccacg
     6301 gtttaccatt gatatccgcc gaagcacggt tgtagagcac gcgacggttg agcggccacg
     6361 cccaggccca tcccagcgta ttccccagac cggatgggtc ggagttatcg cggttagcca
     6421 tctggttgcc ctgctctgtc cagctaccgg tgtagatcca gcaagaagat gcggttgtac
     6481 cgtcatcacg cagatgcgca aagctactca gcaactgacc tttcttcgca atcagcacgc
     6541 cattagcgtc atagagatct tccagcgcat agccgttatt ctctttcgcc acttcgtcag
     6601 attgcggttc gtgcggctgc ttgtagttcc agctcatctt catcagcggt tctacgcctt
     6661 taccaccttc ggcctggtac agctcgcgca gatgatggta gatacccgcc agaatttcgc
     6721 cgtcgttacg cgcttcgccc ggcgcgtcct gacctttcca gtgccactgc aaccagcgac
     6781 cggagttagc gatagaacca tcttcttcgg caaagcaggt cgaaggcaga cggaatactt
     6841 cagtctgaat agacgccgga tcgacatcgt tcgactcgcc gtggttctgc cagaaggtag
     6901 aggtttcagt caccagcgga tcgataacca ccatgtactt cagcttgctc aggcagctca
     6961 ccactttgtt tttgtccggg aaggacgcaa ccgggttaaa gccctggcag aagtaaccgg
     7021 tgactttgcc ttcgtccatc atgttgaaat acttgatgac gtcgtaggtc tggtcccact
     7081 tcggcagcca gtcatagccc cagttgttct ctttctgcgc ggcatcgcca tagaaagatt
     7141 tcatcaggct aacgaagaac ttcggatagt tgctccagta gttcacctga tcagccagcg
     7201 tcgctttcgg cgtgttcgct tccagatacg actgcaaatc aacctgtttt tctgacggaa
     7261 gcgtcagata acctggcagg ctggtagaga gcagacctaa gtcagtcagc ccctgaatgt
     7321 tggagtgacc acgcaatgcg ttcacgccgc caccggccat acccatgtta ccgagcagca
     7381 actggatcat cgccatagta cggatgttct gcgcacccac ggtgtgctgc gtccagccca
     7441 gcgcgtacag gaaggtggtt gtgcgatccg gtgcgctggt ggaggccagc acttcacaca
     7501 ctttcaggaa gtcggctttt ggcgtaccgc agatgttttc aacgacgtcc ggcgtgtagc
     7561 gggaaacgtg cgctttcagc aggttccaca cacagcgcgg atgagtcagt gtttcatcgc
     7621 gttttgcata gccgttttca tcgaactgat agttccagga cgacttatca tactggcgtt
     7681 tttcagcgtc gtagccgctg aacaggccgt cttcgaaagc aaaatcatca cgcaccagca
     7741 ggctggcgtt ggtgtaatgt ttaacgtatt cggcgttgat tttgttgttt tcgatcaggt
     7801 agcgcaaaac gccagacagg aacgtaatgt ccgtaccgga acgaataggt gcgtaaatat
     7861 ccgccacaga agcggtacgc gtaaaacgag gatcgacaac gatcaaggtt gcatcgttgt
     7921 tgtttttcgc ttccatcgcc cagcggaaac cgacgggatg cgcttcagca gcgttaccgc
     7981 ccattaccat tacgacgtta gcgtttttga tatccaccca gtggttggtc atcgcaccgc
     8041 gaccaaatgt tggagcaaga cttgctaccg ttggtccgtg tcagacgcgc gcctggttgt
     8101 ctaccgccag catcccgagg gagcgggcaa atttctgggt cagcatcccg gtttcgttgc
     8161 tggcaccgga ggcacacagc ataccggtag aaagccaacg gtttaccgtt acgccctgct
     8221 cgttcttttc aataaagtta gcgtcacggt cagctttcat cagcttcgca atacgggaga
     8281 atgcttcttc ccagctaatg cgctgccatt tgtcagaacc tggcgcacga tattccgggt
     8341 agcgcagacg gttttcgctg ttgacgtaat ccagcaaacc ggcccctttt gggcacagcg
     8401 caccacggct taccggatga tccgggtcac cttcaatgtg ataaatcgct tctctggcgt
     8461 ttttcgcgcc atcacccagg ctatacatca atagcccgca acctacggaa cagtatgtgc
     8521 aggtgttacg gatctcttta gcgcgtaata atttgtagtt tcgcgcctga gccagtgctt
     8581 gcttcggggc aaagcccaat gccgctaccg ttgttccagc cataccgccc gcgcagattt
     8641 taaaaaattg tctgcgactg acgtccattg ctttcctctt ttttcagggg tattactgcg
     8701 cgaggaaact aacagtaaaa ttctgaatct ttttgcgcga aatcaaaaag aacacgtgta
     8761 tgccacaaaa taatccgcct gcgcaaatta acctacctca atagcggtag aaaaacgcac
     8821 cactgcctga caggccagtt aaaaaaatgc tataaaattc agcttaattt ttaacggcaa
     8881 gagagacaaa acagcgagca tgacacgaca aaaagcaacg ctcatagggc taatagcgat
     8941 cgtcctgtgg agcacgatgg taggattgat tcgcggtgtc agcgaggggc tcggtccggt
     9001 cggcggcgca gctgctatct attcattaag cgggctgctg ttaatcttca cggttggatt
     9061 tccgcgtatt cggcagattc cgaaaggcta tttactcgcc gggagtctgt tattcgtcag
     9121 ctatgaaatc tgtctggcgc tttccttagg gtatgcggcg acccgtcatc aggcgattga
     9181 agtgggtatg gtgaactatc tgtggcccag cctgacaatt ctctttgcca ttctgtttaa
     9241 tggtcagaaa accaactggc tgattgtacc aggattatta ttagccctgg tcggcgtctg
     9301 ctgggtgtta ggcggtgaga atgggttaca ttatgatgaa atcatcaata atatcaccac
     9361 cagcccattg agttatttcc tggcgtttat tggtgcgttt atctgggcag cctattgcac
     9421 agtaacgaat aaatacgcac gcggatttaa cggaattacc gtttttgtcc tgctaacggg
     9481 agcatgtctg tggatttact attttctcac gccacaacca gaaatggtat ttagcacgcc
     9541 cgtcatgatt aaactcatct ctgcggcatt taccttagga tttgcttatg ctgcatggaa
     9601 tgtcggtata ttgcatggca atgtcaccat aatggcggta ggctcgtatt ttacgcctgt
     9661 actttcctca gcgcttgcag ccgtgctgct cagcgccccg ctgtcgttct cgttctggca
     9721 aggcgcgcta atggtctgcg gcggttccct gctctgctgg ctggcgacac gtcgtggtta
     9781 aaataattgc cgggtctacg gcccggcagt taatcagata aattccgaca atattaaaaa
     9841 ataaatttca aataccagta attcacctta tatctcattt cgacttcgat cacatatcga
     9901 taaataatat tatttcatct atttttctca ttgtttttaa tcaccattct attttaaaaa
     9961 cagtatgtca tttataaatg acgccgttta aaattcgttt agaaaatacg tttaacgata
    10021 attaaaagga ttctgttaaa tgaaattaaa aatagttgcg gtggttgtaa ctggtttgtt
    10081 agctgcgaac gtagcacacg ctgccgaagt ctataacaag gatggtaata aactcgacct
    10141 ttatggcaag gttaccgctc tacgttattt tactgatgat aagcgtgacg atggtgataa
    10201 aacttatgcc cgtctcggct ttaaaggaga aacgcaaatc aatgatcaaa tgattggttt
    10261 tggtcactgg gaatatgatt ttaaaggcta taacgatgaa gccaacggct cgcgcggcaa
    10321 caagacccgt ctggcctatg ctggtttaaa aattagtgaa tttggctctc tggactatgg
    10381 tcgtaactac ggtgtcggct acgacattgg ttcatggacc gatatgttgc cagaatttgg
    10441 tggcgatacc tggagtcaga aagatgtctt catgacatac cgtaccaccg gtctagcaac
    10501 ctatcgcaac tacgatttct ttggcttaat tgaagggctg aactttgccg cgcaatatca
    10561 aggcaaaaat gaacgtactg acaacagtca tctttatggt gctgactaca cgcgtgccaa
    10621 cggtgacggt ttcggtatct cctcaactta tgtttatgat ggctttggta tcggtgcggt
    10681 atataccaaa tccgatcgga caaatgcgca ggaaagagcc gctgctaatc ctctcaatgc
    10741 ctccggtaag aatgcagaac tatgggccac aggtataaag tatgatgcca acaacatcta
    10801 ctttgcagct aattacgctg aaacattaaa catgaccacc tatggggatg gttatatctc
    10861 taacaaagca caaagttttg aagtggtggc gcaatatcaa ttcgacttcg gcttgcgccc
    10921 atcactcgct tacctgaaat cgaaaggcag agatctgggc cgctacggcg accaggacat
    10981 gattgagtat atcgacgttg gtgcgacgta tttcttcaac aaaaatatgt cgacctatgt
    11041 tgattataaa atcaacctga ttgatgaaag cgactttacc cgtgccgtag atattcggac
    11101 cgataacatc gtcgctacgg gcattaccta tcagttctaa cgtcctctcc tgaccagccc
    11161 ttctctgggc tggtttttga tttaagtgct gctgtatgac tccttaagta aaaatgagca
    11221 atttttgata tttctggtca ttcacctcaa ttaatattat tccatatcct ccggggacat
    11281 cttactataa tatttattga tgagtgatta tgaccaatat taatacagct tgtgtaaaaa
    11341 ataatgccag ttatcaactt aataacgcat tacctaacaa agaaaccatc tccagcaatt
    11401 tttgtgaacg actagaacaa tggggtaata aatcggttaa taatggtgaa gaaagagcaa
    11461 ttgccgtgga gcgaattaaa gaagcttaca attcgaacat ggcatctctt gatttgtcat
    11521 atcttgattt gagtgaatta ccgcctatcc cctcgacagt taatacgtta aatctggaaa
    11581 acaattgtct aacttgtctt gacttagctg acaatgccag tctcgttaat atcaacctca
    11641 gcgttaataa aattaacacg ataacctttc caaaagaatc aaaactggaa aacatttata
    11701 ttgatcataa caatctggaa agtttagatt taaaaaatca gcattcattg gttaatctgg
    11761 aagcgcaaaa taacaaactg acaaaaatta atatttctga tagttataaa ctgaaatttc
    11821 ttaatcttga ttataacaag ctagcatcgc tggatgtctc ccggcaagaa tctctgattg
    11881 agttaagtgc ccatcacaat atgatcactg accttatatt acacaatcat cccaggatga
    11941 aaacaatcac tttaaacgac aaccatattg cacatttaaa cgccaaaaac actacaaaac
    12001 tggaatattt aaacttaagc aataacaatt tactgccaac caatgacatt gatcaactaa
    12061 tatcatcaaa acatctttgg catgtattag ttaacggcat caacaatgat ccacttgcac
    12121 aaatgcagta ctggactgca gtaagaaata taattgatga cactaatgaa gtgaccattg
    12181 atttatcagg acttaattta accactcaac caccagggct gcaaaacttc acatctatca
    12241 atcttgataa taaccaactc acacattttg atgcaaccaa ctacgataga ctcgtaaagc
    12301 taagtctgaa tagtaatgct cttgagtcaa taaattttcc tcaaggcaga aatgtaagta
    12361 ttacacatat atctatgaat aataatgctc tcagaaatat tgatatagat aggctttcat
    12421 cagttactta ttttagtgcg gcacata
//
LOCUS       DAJTZL010000123        12408 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.123, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000123 DAJTZL010000000
VERSION     DAJTZL010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12408
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.123"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            376..1200
                     /gene="gadX"
                     /locus_tag="OO544_002951"
     CDS             376..1200
                     /gene="gadX"
                     /locus_tag="OO544_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HCR6768487.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTDRSAEGIRL"
     gene            1568..2296
                     /gene="gadW"
                     /locus_tag="OO544_002952"
     CDS             1568..2296
                     /gene="gadW"
                     /locus_tag="OO544_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HCR6768488.1"
                     /translation="MTHVCSVILIRRSFDIYHEQHKISLHNESIVLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(2659..5772)
                     /locus_tag="OO544_002953"
     CDS             complement(2659..5772)
                     /locus_tag="OO544_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HCR6768489.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIE
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKDNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPDQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPIEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(5797..6954)
                     /gene="mdtE"
                     /locus_tag="OO544_002954"
     CDS             complement(5797..6954)
                     /gene="mdtE"
                     /locus_tag="OO544_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HCR6768490.1"
                     /translation="MNRRRKLLIPLLFCGAMLTACDDKSAENAAAMTPEVGVVTLSPG
                     SVNVLSELPGRTVPYEVAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELN
                     SAKGSLAKALSTASNARITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAV
                     EQATINLQYANVTSPITGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQD
                     FLRMKEEVASGQIKQVQGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIF
                     PNPNGDLLPGMYVTALVDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIE
                     ASKAIGDQWVVTSGLQAGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            7014..7292
                     /locus_tag="OO544_002955"
     CDS             7014..7292
                     /locus_tag="OO544_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6768491.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYLGNKVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(7293..7820)
                     /gene="gadE"
                     /locus_tag="OO544_002956"
     CDS             complement(7293..7820)
                     /gene="gadE"
                     /locus_tag="OO544_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HCR6768492.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(8619..9191)
                     /gene="hdeD"
                     /locus_tag="OO544_002957"
     CDS             complement(8619..9191)
                     /gene="hdeD"
                     /locus_tag="OO544_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HCR6768493.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            9446..9778
                     /gene="hdeA"
                     /locus_tag="OO544_002958"
     CDS             9446..9778
                     /gene="hdeA"
                     /locus_tag="OO544_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HCR6768494.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            9894..10220
                     /gene="hdeB"
                     /locus_tag="OO544_002959"
     CDS             9894..10220
                     /gene="hdeB"
                     /locus_tag="OO544_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HCR6768495.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            10284..10931
                     /locus_tag="OO544_002960"
     CDS             10284..10931
                     /locus_tag="OO544_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HCR6768496.1"
                     /translation="MTAEFIIRLILAAIACGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLDHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTAAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLVNGNMVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGMAGVKGVSIS"
     gene            complement(10924..11502)
                     /locus_tag="OO544_002961"
     CDS             complement(10924..11502)
                     /locus_tag="OO544_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6768497.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIVFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLGEFQAYGESGYAATQIS"
     gene            complement(11658..12224)
                     /gene="slp"
                     /locus_tag="OO544_002962"
     CDS             complement(11658..12224)
                     /gene="slp"
                     /locus_tag="OO544_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HCR6768498.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
BASE COUNT         3429 a         2753 c         2808 g         3418 t
ORIGIN      
        1 acctgataac ataacgttgt aaaaaccgaa tgcccagcct ttaaaaaaac agctgggcat
       61 tcggttgctt attaatcgca ataatatatt ggctgtctat tcatcgtgtt gataagatat
      121 atgttatatg aatgtttatt aaatgtagcg attgccttta atgtcacgaa aagggattat
      181 ttgcttgcta ttaatttcac tgcgttctta aacacatttt tgtattataa atccagtcat
      241 cctgcccgta gaacatcaca cattatcatc ctgttctccc gcttcgttta aatttattta
      301 tcaatcaatt tgacttaaga gggcggcgtg ctacattaat taacagtaat atgtttatgt
      361 aatattaagt caactatgca atcattacat gggaattgtc taattgcgta cgcaagacat
      421 aaatatattc tcaccatggt taatggtgaa tatcgctatt ttaatggcgg tgacctggtt
      481 tttgcggatg caagccaaat tcgagtagat aagtgtgttg aaaattttgt attagtatca
      541 agggatacgc tttcattatt tctgccgatg ctcaaggagg aggcattaaa tcttcatgca
      601 cataaaaaaa tttcttcatt actcgttcat cactgtagca gagatattcc tgtttttcag
      661 gaagttgcgc aactatcgca gaataagaat cttcgctatg cagaaatgct acgtaaaaga
      721 gcattaatct ttgcgttgtt atctgttttt cttgaggatg agcactttat accgctgctt
      781 ttgaacgttt tgcaaccgaa catgcgaaca cgagtttgta cggttatcaa taataatatc
      841 gcccatgagt ggacactagc ccgaatcgcc agcgagctgt tgatgagtcc aagcctgtta
      901 aagaaaaaat tgcgcgaaga agagacatca tattcacagt tgcttactga gtgtagaatg
      961 caacgtgctt tgcaacttat tgttatacat ggcttttcaa ttaagcgagt cgcagtgtcc
     1021 tgtggatatc acagcgtgtc gtatttcatt tacgtctttc gaaattatta tgggatgacg
     1081 cccacagagt atcaggagcg atcggcgcag ggattgccga accgtgactc ggcggcaagt
     1141 attgttgcgc aaggaaattt ttacggcact gaccgttctg cggaaggaat aagattatag
     1201 aattttactc agacataaaa aaaacccggc ataggggacc gggaagagga tagtctgccg
     1261 tctccagact aataaaccgt tataacactc cctgttggca cgggaaactt tgtgctctca
     1321 gtaagttaaa tataactttt actggaaata agatcagcca tttttttata aacataagct
     1381 atacgctgtg cgaaaatata ttcgtgctgc atttacttat tatcaattaa ctgttatgca
     1441 aaactacttt gtggataaat tttggtccta ccaaatctgg cagttttttc gctaagaaac
     1501 agtctggcaa catttcatta gtatactgaa attgaaataa tcgcagtatg aaatataagg
     1561 gataatcatg actcatgtct gctcggtgat cctcattcgt cgttcattcg atatttatca
     1621 tgaacagcat aaaatatcgc tgcataacga gagtatcgtg ctgctggaga aaaatttggc
     1681 agacgatttt gcgttttgtt caccggatac gcgacgactg gatatcgatg agctgacagt
     1741 ttgccattac ttacaaaata ttcgtcagct accacgcaat ttagggttac atagcaaaga
     1801 ccgtctatta attaaccagt caccccccat gccgctggtg acggcgattt ttgatagctt
     1861 caatgaatcc ggggtaaatt caccgatact gagcaatatg ctctaccttt cctgtttatc
     1921 gatgttttct cataagaaag aactgatccc cttacttttc aatagcatca gcactgtttc
     1981 aggaaaagtt gaacgcctta ttagctttga tatcgccaaa cgttggtatc tgcgcgatat
     2041 cgcggaaaga atgtatacca gcgagagtct cataaaaaaa aagttgcagg atgaaaatac
     2101 ctgtttcagt aaaatattac tcgcttccag gatgtcgatg gccagacgat tactcgagtt
     2161 acgtcaaatt cctctgcata ctattgcaga aaaatgtggc tatagcagta cgtcgtactt
     2221 tataaacaca tttcgacaat attatggtgt aacgccacat cagtttgcgc aacattcgcc
     2281 aggtaccttt tcctgacata ttttgcattt gaatattggt caggatctca cacctgcttc
     2341 atgtgaaact cttccctgat gatttctgcc gggctaccgg ctagttctct ttcgcagttt
     2401 gccaggcggt ataggtattt tcaggaggaa aagactcggc atgtttggga ttattaagct
     2461 gacaattcat acgattacgg gaatgtgggt cagtattgtg ctgttcaaat tgatgacgaa
     2521 tggctggagt ggtttctact ttcagtgctg tgtactaagc ctggtattcc tgacggttag
     2581 ctggttgttg agtggtgaat ggttagcagg aaagagtaag gctgaacctt cacgttcaac
     2641 cttactctca tttacacgtt acgctttttt aaagcgggca aagagatgtt ccactacaac
     2701 gaaaaagacc ggaacgaagt aaattgccag cactgttgcg gcaaacatcc cgcccatcac
     2761 gccggtacct accgcgtttt gcgcgccaga accggcacca tgactgataa ccagcggcag
     2821 cacgccgaga ataaaggcga gagaggtcat caggattggg cgtaaacgca tccgcgccgc
     2881 ttcgatgatt gcctctatcg gcgttttccc ttctttctgc atcatctcaa cggcaaattc
     2941 gacaatcagg atggcgttct tggcggaaag cccgatggtg gtcagcaaac caacctggaa
     3001 gtagacgtca ttacttaagc cgcgcagatc ggtggccagt aatgcgccaa cgacgcctaa
     3061 cggaacaacc aacatcaccg agaacggaat tgaccagctc tcatagagtg cggcgagggc
     3121 gaggaacacc acgaccagtg aaatggcata cagtgcagga gcctggtttg aggataacgc
     3181 ttcctgatac gacagtccgg tccatgagta gccgacgcct gccggaagtt tagcgaccag
     3241 gtctgccata aatttcatag cgtcaccggt acttttcccg gccgccgctt cacctaaaat
     3301 ctccattgac gggatgccgt tgtagcgttc caatcgcggt gaaccatagg tccattcggt
     3361 agacgagtag gcagaaagcg gtgccatcgt gccagaggcg ttgcgtacat accattggtt
     3421 gatgttatcc ggcaacatac ggaacggcgt gcctgcctgg acatacactt ttttcacccg
     3481 cccctggttg aggaagtcgt tcacgtagct gctgccgaag gcggtggaaa ttgtctggtt
     3541 gatatcagac agcgccacac ccattgcctc agctttcgcg gcgttgacgt tcactttgaa
     3601 catcggcgta tcttccaggc cgttcgggcg taccccggtg acctggtcag gcgattgcgc
     3661 tgccagtgat aacagctcgt ttcgtgcctg ggttagtttt tcgtgcccca ggttaccgtt
     3721 gtccagcagt tccatatcaa aacctgacgc ggtacccagt tcagccaccg cgggtaagtt
     3781 gaacgggaag acgacggctt tattgatact gcttaacgca atcattgccc gctgaatgat
     3841 cgcggtaacc gagttttcct caccgacacg ttcagaccac ggcttgagac tgataaacgc
     3901 caggccgttg ttttgcccct ggccgctgaa gccaaagccg ccaacggtaa acaccgactg
     3961 aacattatct ttctcttttg taagataata atccgtcacc tgttgcagca ctttcgtggt
     4021 gttaaccatg gtggcaccgg aaggtaactg cgcggtggtc ataaataccc cctgatcctc
     4081 ttctggtaag aaagaggtcg gtgtacgcag gaacagcacc gccatcccgg cgcaaatcag
     4141 caggtagacc accatgtagc gaccggtaca acgcaacagc gagcgggtgc tatcggtata
     4201 gtgctgagtt gatttttcaa acagcgtgtt gaagcgtgcg aacagggcgt taggtttgtg
     4261 accgccttcc ggcgcggctt tcagaatggt ggcgcacagg gcaggggtca ggctcattgc
     4321 cacaaatact gaaagcagca tggaggagat cagcgtgatg gagaactggc ggtagatctc
     4381 cccggttgcg ccgctcataa aggccatcgg cataaacact gcggaaagaa caacagcaat
     4441 accgaccagc gcacgttgga tctgccccat cgatttatgc gtcgcttcct tcggtggtag
     4501 cttatcttcc gcaatgacac gctcgacgtt ctccaccacc acgatggcgt catccaccag
     4561 taaccctatc gccagcacca tcccgaacat agttaacgtg ttgatggtga aaccgaccgc
     4621 cgacaagatc gcaaacgtcc cgagaataac caccggtacg gcaatcgtcg ggatgattgt
     4681 ggcacggaaa ttctgcaaaa acagatacat gaccaggaag acgaggatga tagcctcaac
     4741 cagtgtttta aaaacttcct gaatagaaat ttcgataaac ggcgtggtgt cgtaaggata
     4801 aaccgtcttc agacttgcgg ggaaataggc tgataagcgg ttcagctcct ctttgactgc
     4861 ccgcgaggta tccagggcgt ttgctccggt agccagtttg atggcgatcc cggcagcagg
     4921 tttgccgtta tagcgcgcca cggtggaata atcttccgct ccaagttcga cgcgggcgac
     4981 atcacgcagc agcacttgcg aaccatcttg ctgaactttc aacaggattt tgccaaattc
     5041 ttccggcgtt tgcagacgcg tctgcacaat gatcgaggcg tttagttgct ggtctgccgc
     5101 ctgtggcatg ccacccagtt gaccaccgga aatctggttg ttttgcacct taatctggga
     5161 aataacatcg gaaggtacca gattgtattt attgagtttt tgcgggtcca gccagatacg
     5221 catggcgtac tcggaaccaa agagctgtac gctaccaacg cccgcggtac ggcttagcgg
     5281 gtctttgata ttagacgcta catagtccgc gatatcgtac tggttgaggc tgccgttatc
     5341 agaaataaac gccgctacca tcagaatatt actgctcgac ttatcaacgc taatcccctg
     5401 ctgctgcacc gcttcaggta atgaaggcat agcgagttgc agtttatttt gcacctgaac
     5461 ctgtgcgata tcaggagatg tcccagtctc gaaggtcaga gtgatagagg cattacccgc
     5521 cgcatcactg gttgaagaca tgtacatcag gccatcaagc ccattcatat tttgctcaat
     5581 cacctgagtg accgagtctt ctaccgtttg cgcatcggca cctggatagg tagcgctgac
     5641 ggtaatggtc ggtggcgcaa tctgcggata ctgcgcaacc ggtaagttca tgatcgccag
     5701 accacctgca agcatcataa taatggcaag tacccaggca aaaaccgggc gatcaataaa
     5761 atagttagcc atgaaagtcc ccttaagcct gcaacgttat tgtttcgatt cggtgctggc
     5821 gttttcctgg ctggaggaaa ttgctcgtgc tttgataccc ggacgaatgc gttgcaaacc
     5881 ggaaacgatc acccgatcgc cagcctgcaa gccagaggtg acaacccact ggtcgccgat
     5941 ggctttgctg gcttcaattt cgcgtagctg cacaacatcg tctttatcca gaatgagcgc
     6001 cgttgcttta ccctgggcgt tgtgagtgac gccttcctgc ggcaccagta atacattctg
     6061 gcggctacct tcatccacta atgccgtgac gtacatgcca ggcagcaagt caccatttgg
     6121 gttggggaaa atcgcccgta acgtcacgga gcccgtggtt tcatccaccg tcgggtcgga
     6181 gaatttcagt gtgccggtct ggctgtagcg tttaccattt tccagattga gctgtactgg
     6241 cgtactgccc tgaacctgtt tgatttgccc actggcgacc tcttctttca tgcgcaagaa
     6301 atcctgcacc gactgcgtga gatcgacata aatcgggtcc agacgttgta cggtaaccag
     6361 cgaatctgcc tgattagcgg taacgagtgc gccgacggtc accgacgatt tcccgctgac
     6421 gcccgtaatc ggcgaggtga cattcgcgta ttgcagattg atcgtcgcct gttcaacagc
     6481 cgctttggcg acggtgacat tggcttctgc ttcattcagc tgggtgcgcg cggtgtcgta
     6541 atcctgacgg ctaacgtagt tggtcttcag caacgatgcc tggcggttaa aggtgatgcg
     6601 ggcattgctg gcggtagaga gcgctttcgc cagcgagcct ttggcggagt ttagctcggc
     6661 ctgtaaaggt gcaggatcaa tctgatacag cgaatcgccc tggttcactt tatcgccttc
     6721 gataaagttg cgtttaatga taataccgcc cacctgggga cgtatctcgg caacttcata
     6781 aggaacggtt ctaccgggca attcgctcaa cacattgacc gaaccggggg agagtgtgac
     6841 gacaccgacc tcaggcgtca tggcggcggc gttttccgcc gatttgtcat cgcaggcggt
     6901 gagcatcgcg ccgcagaata acaacggtat taacagcttt cttcttctgt tcattttagt
     6961 ccctgaaaat tcttgagaaa taacggtttt ttaaattgcg cgcgaggaga gatatgaggg
     7021 atttacagac cagcggcata gtggggttgt cagccagtaa ggtgggatac aggcacagtg
     7081 atcgacatgg tgaggtcaac gacaaaaaaa atgttgccgt tctgccaaca gttcctgcca
     7141 gcattcgggc aacgaagggt tctactggtg gatacacata cctggggaat aaagtcgttt
     7201 tagcctctcc tttagtaatc accgggggta atgaaatcag catctgcatc cctcgtcatg
     7261 ccagccatca atttcagttg cttatgtcct gactaaaaat aagatgtgat acccagggtg
     7321 acgatgtcgc tcatacgtct tagtttcagc ttgtacataa tgttgaattt ctgaatcttt
     7381 aatgtttgtt gcgaaatatt gagcgtcgac gtgatattgc tttcattttt atttttcaga
     7441 atcagttgca taattttgaa ctgattatgt gaaaaacata attgtgaatt cttatggggc
     7501 aagtgtttac cataagtaat ctcaagaaga tttttgatac tttctttgcg gctaacaaaa
     7561 taaataggcg ctttagcttt tagtttactg atgtgataag gtgccaaaac aataattctc
     7621 tcggcatcta atttctccag aaatttaatc gcttcttcat caaggatatg attgtgatag
     7681 gtatcaatga taaccttgaa gggtttattg cctacttttt tgatctctga cagggaattg
     7741 atttttatca tttcgtgatt atctttcaac tgccaaaagc cctgtaaaag aaaagaatct
     7801 ttcgtcatga gaaaaatcat aacttgctcc ttagccgtta tcgtttcgaa catgtcatcc
     7861 ttttcattat ttacatcctt gtccgaatcg ttgttcaata tagtaaacgc ctataagaac
     7921 tttacccttc gtgaactaat acgtctatta acactaatca atacttcaga tttatccatc
     7981 acgcactcca tatattgatc ctaagtaaga ttcctggttg ttatcagctt gtaaatagta
     8041 aacaaaaagt taacgtttgc ggattactgc ccaagaataa gttatcaata accagtttgt
     8101 gatctctgaa gaatattact aaagttaaaa tgtaatccga tttaaatatc gagtctcctt
     8161 gtttcgactt aagctggcaa ttggattgcc agctttcttt taacgcattg aaattacctg
     8221 agtaaataac gcattcatgt cttcaggttt ttatatagcg tctggagaca cggatattta
     8281 tgcaatgtaa tcaacgccag ttgcaaaaaa atgaacccgg attacttatt taaccgccca
     8341 tagcatcaac gcttacggaa ctctttgttc actttcccac agcactttta aaacattaaa
     8401 cctgactacg gaaaatatca gccatgataa tgtttgaaat ggctaatttg ccatggagtg
     8461 aaaaaaatta gatgaaattc agtaggttga aataatcact agcaggtaat tatttcaatg
     8521 atagtgcgca attgatctac aacactccgt agcggagaga gtattaatcg gatcatagtc
     8581 atatcaagtg actatgatcc gggtgacaac cgggataatt attgctgctt aacgaacaaa
     8641 ctggcgaagc tgaacaggct ggcggcgcta aatatcagtt caattcccac cagtgtggaa
     8701 accagcgtta cagacaccat cggcgttgca ccaaggaata tccaggcaat gacgatatcc
     8761 agcacaccaa taacgagctg tagccagctg cctttcattg aacgctgacg ataccaactc
     8821 atcaggcgaa taacccctgc aacacagaac aaaccggcaa taaatgccgc aatggcaaaa
     8881 atgcccagct ccggtgcgcg gatgaagaaa tagccgatca ataaataggc gactgcgacg
     8941 aggaaaccgg ataataccgg ccagaaatta tgactgcggt tgctgaataa cccgacaata
     9001 agcgcaatgc ccgagcagat taataatgca cccactactg tgcttaaaat atcgccagag
     9061 acgaacggga aactgataca cagcaacccg acgataaaca gtagcacggc aataaactgg
     9121 attgctctgc gatgtttttt aagcatctcc agatcaaact tcaaaattgt tgccttatct
     9181 atatataaca tagaaccacc ctataaaatt aagaagaaaa tcccctgcta tcaatctatg
     9241 ccaaaaacgc gtctaagaat gtagtcgatt taataaaaat ttcctaatta cagtatctga
     9301 tgcatctgta actcattgta ttgaaataaa aatatctgat tttgatattt tatatcaaca
     9361 tgacatatac agaaaaccag gttataacct cagtgtcgaa attgattcgt gacggctctt
     9421 tcactttata gttgaggata ttacgatgaa aaaagtatta ggcgttattc ttggtggtct
     9481 gcttcttctg ccagttgtga gcaatgcagc ggatgcgcaa aaagcagctg ataacaaaaa
     9541 accggtcaac tcctggacct gtgaagattt cctggctgtg gacgaatcct tccagccaac
     9601 tgcagttggt tttgctgaag cgctgaacaa caaagataaa ccagaagatg cggttttaga
     9661 tgttcagggt attgcaaccg taaccccagc tatcgttcag gcttgtactc aggataaaca
     9721 agccaacttt aaagataaag ttaaaggcga atgggacaaa attaagaaag atatgtaatt
     9781 ccgggaatgc gttacatcgt acttccttgc atattgaaca ggccggaata tcttctttaa
     9841 aagcagctat tcctcctgtt catatataat ctctatattg aatgggttac aaaatgaata
     9901 tttcatctct ccgtaaagcg tttattttta tgggcgctgt agcggctttg tcactggtga
     9961 acgcacaatc tgcgctggca gccaatgaat ccgctaaaga tatgacctgc caggaattta
    10021 ttgatctgaa tccaaaagca atgaccccgg ttgcatggtg gatgctgcat gaagaaacag
    10081 tatataaagg tggcgatacc gttactttaa atgaaaccga tctcactcaa attcctaaag
    10141 tgatcgaata ctgtaagaaa aacccgcaga aaaatttgta taccttcaaa aatcaagcat
    10201 ctaatgactt gccgaattaa tgaggtacaa gtaaaaagga gtagcaagtt gagccatctt
    10261 gctgctcctt tttgcatttt tatatgacag cagaatttat tatacgtctt atactcgcgg
    10321 caattgcctg tggcgctatt ggcatggaaa ggcaaatgcg cggcaaagga gcagggttac
    10381 gcacacatgt attaattggc atgggaagcg ccctgtttat gattgtttcg aaatatggtt
    10441 ttgctgacgt gctgtcttta gatcacgtcg gactcgaccc cagccgtatt gctgctcagg
    10501 tggtgacggg cgtcgggttt atcggtgcag gtaacattct cgttcgtaac caaaatattg
    10561 taggtctgac gacggcagcg gatatctggg tgaccgccgc cataggtatg gttattggca
    10621 gcggtatgta cgaactgggt atttatggtt cagtgatgac cttgttggtg ctggaagtct
    10681 tccatcaatt aaccttccgc ctgatgaaca aaaattacca tttacagctg acattagtga
    10741 acggtaatat ggtatcaatg ctcgactggt ttaagcagca aaaaataaaa actgatttag
    10801 tttcgttgca ggaaaatgaa gatcatgaag tggtcgccat tgatatccag cttcatgcca
    10861 caacgtcgat agaagacctg ttgcgattat taaaaggtat ggcaggggtg aaaggcgttt
    10921 caattagcta atctgtgtgg cagcgtagcc agactcaccg taagcctgaa attcacccag
    10981 ataatcaata tgctgatggc gtaccagctc attgatccgc ttaagattga ggatcatcat
    11041 gatattgtgt ttatggcagt agaacgtttt tagcgatatt ttcagtttct ccagaatatc
    11101 ttcagttgtc atttgattcg caatgcagct cagaacctga tgttgcttgc gcgaaagtga
    11161 cacattcgct tcaggcacag agcagtaact gccattgatg agtgcgacaa aatcaatgat
    11221 agtgatagtc cgcggaacaa aggtcaccgg tactttcccc agacagcgtt taatattaaa
    11281 gggggagaaa acaatgacat ggacaggttt taatcgttca atttgagtga gaaaattaga
    11341 atgaaataca ttattcatta atgtatcaat aatgacgaag gcattctgat gcaacattac
    11401 gtcgatctct tcttcgttct gtatatgaac gacattacct ttactcagaa tgtttttcat
    11461 cgcggtgaag aacatcgtat ccctggtaat tataagaaac ataaccgact cctggtgcgg
    11521 acattaactt tataatcaat ttgccaaagg taattaacag gacagggctt ataataataa
    11581 tagttagctt actgtatatt aattttatgt aatacatgat gcagatgctg agaaagcatc
    11641 tgcatctttc ggtggtgtta tttgaccagc tcaggtgtta cctgactcac cgcattggtg
    11701 tagtaaggcg caccccagcc cggttccggc cagaatgcac catagccgta atcccacagg
    11761 ttataggtgg tattaaccac ttctctcaaa tgccacacct ggatgccctg catattcact
    11821 tccaggaagt tatacgggac tttattgata aagccaggtt gttcaccctg aatggtaccg
    11881 aggatggtaa caaagtgatt acgatagttc actgggtcaa ggaagccgct ttgtctggcg
    11941 agcagtcggc cctgatagtt ggcttcaata tcaggtttcg catagctatc caacggtaat
    12001 acagcgattt ctaacaacgt atccgttttg ccattgataa cgttgataac cttcccacca
    12061 aagcgcgctt gttgaccaac atataacccc ggctggttat gaacagcaac aaaacttttt
    12121 tgaatatcag gttggttatt gcctttgata ttttgcggaa ttgaactaca tgcggcaagc
    12181 aaaaatgaaa ggctgaggat gagtgcacct tttgtcatgt tcatgttact atccttatca
    12241 acaaatatta aaaccataat agatatttct ttatagtttt catctgattc tgaggcgata
    12301 tgcctgatag taacgtaaac ctgtctgatg tttatatccc ttttgtttag ttcaattcca
    12361 ttgattttgg ggcgtggcat gagtatccat cgccccataa tataatcc
//
LOCUS       DAJTZL010000124        12180 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.124, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000124 DAJTZL010000000
VERSION     DAJTZL010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12180
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.124"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..510
                     /gene="csgD"
                     /locus_tag="OO544_002963"
     CDS             <1..510
                     /gene="csgD"
                     /locus_tag="OO544_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HCR6768499.1"
                     /translation="DDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPE
                     DYPYRDIENWPHINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYN
                     STESALLTHREKEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAV
                     SWANDNLRR"
     gene            515..904
                     /gene="csgE"
                     /locus_tag="OO544_002964"
     CDS             515..904
                     /gene="csgE"
                     /locus_tag="OO544_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HCR6768500.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            929..1345
                     /gene="csgF"
                     /locus_tag="OO544_002965"
     CDS             929..1345
                     /gene="csgF"
                     /locus_tag="OO544_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HCR6768501.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            1372..2205
                     /gene="csgG"
                     /locus_tag="OO544_002966"
     CDS             1372..2205
                     /gene="csgG"
                     /locus_tag="OO544_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HCR6768502.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPRAQSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFIPLERQSLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKAERQNDILVKYRHMSVPPE
                     S"
     gene            complement(2270..2761)
                     /locus_tag="OO544_002967"
     CDS             complement(2270..2761)
                     /locus_tag="OO544_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HCR6768503.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(2863..3417)
                     /gene="ycdY"
                     /locus_tag="OO544_002968"
     CDS             complement(2863..3417)
                     /gene="ycdY"
                     /locus_tag="OO544_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HCR6768504.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMAQVSADYNALFIGDECAVPPYRSAWVEDVTEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(3441..4178)
                     /gene="ycdX"
                     /locus_tag="OO544_002969"
     CDS             complement(3441..4178)
                     /gene="ycdX"
                     /locus_tag="OO544_002969"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HCR6768505.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(4233..5171)
                     /gene="ghrA"
                     /locus_tag="OO544_002970"
     CDS             complement(4233..5171)
                     /gene="ghrA"
                     /locus_tag="OO544_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HCR6768506.1"
                     /translation="MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLNPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVEYISRTIAQLEKGERVCGQVDRARGY"
     gene            5405..5492
                     /locus_tag="OO544_002971"
     tRNA            5405..5492
                     /locus_tag="OO544_002971"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5439..5441,aa:Ser,seq:gga)
     gene            5537..5673
                     /locus_tag="OO544_002972"
     ncRNA           5537..5673
                     /ncRNA_class="other"
                     /locus_tag="OO544_002972"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(5764..6450)
                     /locus_tag="OO544_002973"
     CDS             complement(5764..6450)
                     /locus_tag="OO544_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HCR6768507.1"
                     /translation="MSFMAFVTCLTIAFIAGFAVARKLTLRKVASSVIIAKSQPVPSS
                     ATSPAAKASTRSYIPQKTYRDSRWQERDELFQGMVAEGILLERKEWLITRCEKMYFDN
                     LEKWFGHYCHIHCQVSLGRLINFPEQPEFSEEERKRFFAIYNGMAMDYVLVSKKNNKI
                     VCVIELDDPSHERADRIIRDRWLDTIMTLARVPFVRASVTKLEEIPPVWETRDKIRPL
                     VAATKSEPVH"
     gene            complement(7031..7819)
                     /gene="phoH"
                     /locus_tag="OO544_002974"
     CDS             complement(7031..7819)
                     /gene="phoH"
                     /locus_tag="OO544_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HCR6768508.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMGGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGGGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(8440..9711)
                     /gene="efeB"
                     /locus_tag="OO544_002975"
     CDS             complement(8440..9711)
                     /gene="efeB"
                     /locus_tag="OO544_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HCR6768509.1"
                     /translation="MQYKDENGVNEPSRRRLLKGIGALALAGSCPVAHAQKTQSAPGT
                     LSPDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHV
                     SRSKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLAN
                     PRTAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFSLPGVKDANDYLGSALLRV"
     gene            complement(9717..10844)
                     /gene="efeO"
                     /locus_tag="OO544_002976"
     CDS             complement(9717..10844)
                     /gene="efeO"
                     /locus_tag="OO544_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HCR6768510.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYAEQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(10902..11732)
                     /locus_tag="OO544_002977"
     CDS             complement(10902..11732)
                     /locus_tag="OO544_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HCR6768511.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGATASRSA
                     "
BASE COUNT         3218 a         3153 c         2812 g         2997 t
ORIGIN      
        1 gacgatatat cttcaggctc tattattctt ctggatatga tggaagcgga taaaaagctt
       61 atccattatt ggcaggatac tttgagcagg aaaaacaaca atatcaaaat attgttgcta
      121 aatacgcctg aagattaccc gtaccgcgac attgaaaact ggcctcatat caacggcgtt
      181 ttttatgcca tggaggatca agaacgtgtt gtcaatgggt tgcaaggcgt cctgcgcggc
      241 gaatgctact ttacgcaaaa gcttgccagc tacttgatta cgcattcagg taactatcgt
      301 tataacagca cggaatcagc cctccttact catcgggaaa aagagatcct gaataaactg
      361 cgtatcggcg cgtctaataa cgagatcgct cgttcgttgt tcatcagcga aaatacggtt
      421 aaaacgcatc tttataatct tttcaagaag atagccgtaa aaaaccggac acaagcggtt
      481 tcctgggcaa acgataacct caggcgataa agccatgaaa cgttatttac gctggattgt
      541 ggcggcagaa tttctgttcg ccgcagggaa tcttcacgcc gttgaggtag aagtcccggg
      601 attgctaact gaccatactg tttcatctat tggccatgat ttttaccgag cctttagtga
      661 taaatgggaa agtgactata cgggtaactt aacgattaat gaaaggccca gtgcacgatg
      721 gggaagctgg atcactataa cggtcaatca ggacgttatt ttccagactt ttttatttcc
      781 gttgaaaaga gacttcgaga aaactgtcgt ctttgcactg attcaaactg aagaagcact
      841 aaatcgtcgc cagataaatc aggcgttatt aagtacgggc gatttggcgc atgatgaatt
      901 ctaaataaaa aattgttcgg aggctgcaat gcgtgtcaaa catgcagtag ttctactcat
      961 gcttatttcg ccattaagtt gggctggaac catgactttc cagttccgta atccaaactt
     1021 tggtggtaac ccaaataatg gcgctttttt attaaatagc gctcaggccc aaaactctta
     1081 taaagatccg agctataacg atgactttgg tattgaaaca ccctcagcgt tagataactt
     1141 tactcaggcc atccagtcac aaattttagg tgggctactg tcgaatatta ataccggtaa
     1201 accgggccgc atggtgacca acgattatat tgttgatatt gctaaccgcg atggtcaatt
     1261 gcagttgaac gtgacagatc gtaaaaccgg acaaacctcg accatccagg tttcgggttt
     1321 acaaaataac tcaaccgatt tttaagcccc agcttcataa ggaaaataat catgcagcgc
     1381 ttatttcttt tggttgccgt catgttactg agcggatgct taaccgcccc gcctaaagaa
     1441 gccgccagac cgacattaat gcctcgagct cagagctaca aagatttgac ccatctgcca
     1501 gcgccgacgg gtaagatctt tgtttcggta tacaacattc aggacgaaac cgggcaattt
     1561 aaaccctacc cggcaagtaa cttctccact gctgttccgc aaagcgccac ggcaatgctg
     1621 gtcacggcat tgaaagattc tcgctggttt ataccgctgg agcgtcagag tttacaaaac
     1681 ctgcttaacg agcgcaagat tattcgtgcg gcacaagaaa acggcacagt tgccattaat
     1741 aaccgaatcc cgctgcaatc tttaacggcg gcaaatatca tggttgaagg ttcgattatc
     1801 ggttatgaaa gcaacgttaa atcgggcggt gttggggcaa gatattttgg gatcggtgca
     1861 gacacgcaat accagctcga tcagattgcc gtgaacctgc gtgtcgtcaa tgtgagtacc
     1921 ggtgagatcc tctcctcggt gaacaccagt aagacgatac tttcctatga agttcaggcc
     1981 ggggttttcc gctttattga ctaccagcgc ttgctggaag gggaagtggg ttacacctca
     2041 aacgaacccg ttatgctgtg tctgatgtcg gctatcgaaa caggggtcat tttcctgatt
     2101 aatgatggta tcgaccgtgg tctgtgggat ttgcagaaca aagcagaacg gcagaatgac
     2161 attctggtta aataccgcca tatgtcggtt ccaccagaat cctgacagat aaaaatgccg
     2221 gaattaataa tccggcattt tactgtttaa gcgaaagcgt gagtgcccct cacgctttat
     2281 ttttgatttc ctgctcacgg tgagccgtct ttgcactacg cgccgccagc cacaggccgc
     2341 tgtttttcat tgcgtaacca aataccagcc caagcgccag cgaaggtaac accagtttcc
     2401 agtcaccctg cccggcaaac gtcgcgcatg cgcctataaa cgtccccgga acaaatgaaa
     2461 gcagcagctg tttggcctga atacacatca gaaacgccac aataccggtt atgacataac
     2521 cgagaatttc cagatgtggt gccagtgcac taccgtaaat aatgaccatc gcccacacca
     2581 cgccactaag cagggttgca gcggagatcg ccagcccttt caacccacct tgcgggcagg
     2641 caaagtaggc cgtacagccc aagaagcccg cccagcttag taagcccagg gaaacagcca
     2701 cccatcccca gataccggag agaatgcctg ttgtgattgc aatagagaga agtatgttca
     2761 tgccgcgcat gatagcagaa aacgcggcca tgaatgagat cgagcacaca ttttatgcaa
     2821 caacaaatta tctgttgcag ttatgagtga cgatgatcac atttactctt cagaatcttc
     2881 ttccagctcg tcccacattg cactaatggc atcacgggtt agcggtgcca tggtgcgcca
     2941 gaaaggcgtg gttgcatggg cctccacttt gccgaggaac gcaccacacc agggtaacag
     3001 atactcactg aacagtgttt ccagtgcttc gctctcatct tccgttgact gatcttccag
     3061 ccaggaagcc gcaagcagca atgtgccgat gtgatcggct ggcgtatccg ccagtggcat
     3121 ccctcgttcg gaaagaaaag cgcgcacttc cgcttccgtt acgtcctcaa cccatgcgct
     3181 acgatatggc ggcactgcac attcatcgcc gataaacaac gcattgtaat cggcagagac
     3241 ttgcgccata tcacagcttt tctgtaaacg cgtcagtaac tcatcctgct ccagtggcca
     3301 gttcgcagcc agtttcccct cacgaatcag ggtaaacagc ggcaccagta aaggatcttg
     3361 cggttggcgg taatagagcg aacccagcac acgacagagg atagaaaact cgttcattaa
     3421 tatatttcca ttactagtaa ttaaaggtct gcaaattccg caatcggtgc cataccgcga
     3481 gattcaagga agttcagtaa gcggcgcgga gaaacattca aaatgcgctc tggcggaaaa
     3541 tctaccgcgt cgaggatttt aagacactct tcaaattccc ccatggtaaa cgcggtgtga
     3601 gaatccgagc ctaatgccac ccaacctcca gcatcacgta ccgctgcggc taccgcacga
     3661 cagttgtctt cactgccctt acgtgagtgt aaaaatgaag aattattgat ttccagcgcc
     3721 acctgatgtt tcgcggctgc ttcagcaacg gctttcacat ctatttcata tttgggattt
     3781 ccgggatggc ttattatatg cacattgccg ctggcgatag tggcgatcat cgcttgtgta
     3841 ttggtcgctt tgtcatgtgg tgcaaaaacc ggctcatgaa aaccggcaat aatcagatcc
     3901 agcgagtcaa acattttacc gctgcaatca atttcaccat caacattttt aatgttagct
     3961 tcgatgccgc gcaggatccc taccccatca accactcgcg gccagatacg catgttaatg
     4021 aagtgccagt gatgcggagc atcttccata tccgggccat gatcggtgat cgcaaaaagt
     4081 ttaatgccct tttgtttggc ctgggcaatg taatcactca atgtgctata tgcatgtgtg
     4141 ctggcaacgg tatgcatatg aaggtcgacg ggatacataa ctctctcctg tattcaattc
     4201 ccgccaagga tagcaggaat cctgatgctt tattagtagc cgcgtgcgcg gtcgacttgc
     4261 ccgcagaccc tctccccttt ttcgagctgg gcaatggtgc gagaaatgta ctccacagct
     4321 tcagcgggac gggtaatcgc ggcgacatgt ggtgttatcg tcacgcgtgg atgttgccag
     4381 agcggacttt caggcggtaa gggttcacga ttaaaaacat ccaacattgc gcctttaact
     4441 ttgccgctat ccagcgccgc gagcaggtca tcttccacaa catgaacacc acgcgccagg
     4501 ttgaggagat acgcgccatc cggtaatttt tcgagtaatt gttgattaat aatgccgacg
     4561 gtttcagggg tattcggtaa caaattaatc aatacccgac attggctcag aaatgcagac
     4621 agttcttccc gtccggcaaa gctttgcacg ccaggccacg atttacgggt tcgactccag
     4681 caacgcagcg gaaagcgcca ggtttgcaga ctctgagcaa ctttactgcc cagtacgcct
     4741 gcgcccaaaa tgccgatggt aaaatcttcc cgatgatatt caggcagcgg ttgccaatgc
     4801 gaactatttt gctggatgcg ataatcgtca aaacgtcgaa accaatgcag cacctgactg
     4861 acagcatatt cctgcatttg ctcgcccata ccggtatctt ccaggcgaaa aagtggaaca
     4921 gaagggttca gcatttcagg gtgtgcctgt agcttgctca aaatagaatc aacaccggcc
     4981 ccgagtgcga acaccgcttt aagatcgcgc cctgccagca tttcaacagg aggatgccag
     5041 actaaagcat aatcagcaga gtcattatct ccgcttttcc atgctctgac tcttgcctga
     5101 ggaatagctt tgcgcagtgc ctcaatccac cattgggtat cgaacgttgg gtgataaaag
     5161 atgatatcca tactgactcc cgaaaagcgt tgtgcgaaat ttattcgcac ttatcgttat
     5221 gatctacaaa ggccaccagc ataacaaatc cgtggtcggt ggcaaaaaaa gcagatttcg
     5281 cttattaaaa ccacacattg attgaagttt gaataaacgc gcgatttttt caaaaagttt
     5341 gttgacctca ggtcatgatt tccctaaatt agcgcccgtt ccagcaagac aggaacgaca
     5401 atttggtgag gtgtccgagt ggctgaagga gcacgcctgg aaagtgtgta tacggcaacg
     5461 tatcgggggt tcgaatcccc ccctcaccgc catatttaaa gaagagctcg tacgaaagta
     5521 cgggcttttt tttcgtatat tgcacacacc ggggggatga gaagccccga ccggggttcg
     5581 acaactggcg acagccagtt ggacagaccg tgaacgcagt gagcgggctg cccgcagggc
     5641 gagcgaagcg agtcaatccc cccctcaccg ccatctttaa agaagagctc gtacgaaggt
     5701 acgggctttt tttcgtatat tgcacacacc ggggggatga gaagccccga ccgggatact
     5761 tagttaatgc accggttcac tctttgtcgc cgcgactaat gggcgaattt tgtcacgagt
     5821 ctcccagacc ggaggaattt cctccaattt agtgacagaa gctcgtacaa aaggcacccg
     5881 tgcaagagtc ataattgtat ccaaccaacg gtcacgaata atgcgatccg ccctttcatg
     5941 actcgggtca tccagctcaa taacacagac tattttattg ttcttctttg aaaccagaac
     6001 ataatccatt gccatgccat tataaattgc aaagaaacgc tttctttctt cttcgctaaa
     6061 ctcaggttgt tctggaaagt tgatcaagcg ccccagagag acctggcaat ggatatgaca
     6121 ataatgtcca aaccattttt ccagattatc gaaatacatt ttttcacaac gcgtgattaa
     6181 ccactctttt ctttccaata aaataccttc ggcaaccatt ccctgaaata actcatctcg
     6241 ctcttgccaa cgagagtcac gataggtttt ctgcggtata tagctccttg tcgacgcctt
     6301 agcggcaggg gaagttgcag atgaagggac gggttgcgat ttagcgatga ttaccgatga
     6361 ggcaactttt cggagtgtca attttctggc aacagcaaaa ccggcgataa atgcaattgt
     6421 caaacaagtt acaaaagcca taaagctcat aattaatttt cacctatcca tgttgattat
     6481 atttcattac caatgtaact cgctgcagcg tgttacatgc caacaatgca acgatcaata
     6541 tatagttagt atcatgtttt gattattaca accatcccta tttagccatt gcggcttgcc
     6601 ttccacaaaa aaataacaac acacaatatt agcaaagaat taattaagat taaccttaca
     6661 tttataatat tatccctaaa gaataaatca gaggaatata ttcggtgtta aacagtcagt
     6721 caaagtatag cccattgttg attaaacctc ataagcaaaa ttggcaccag atctcctgta
     6781 caggttgaat ggccgaaaaa agataggcag atgctcattt ttttggcttc tggaagaaaa
     6841 actctccgcg ctggcagtat tacactggtc tgatgctatg acgaaaaatg cctggtaaga
     6901 gtatctaaac cagactccag ttcagcttat caacacgctc aaagtcgccc ccctgaacgc
     6961 taacctgaag tgaaaatctg ttgaaataaa acaaagcccg gttcgcccgg gctctgcacc
     7021 gataacacac ttaactgtag gcatgcagcg tacgttggca aagtgccgaa cgtacgcagt
     7081 cctctttacc gaaccggacg atcccaacca tttcatcttc ttcgaaacgt tccagcgcgt
     7141 cacttaatcc ggagcacacg ccgcgaggca aatcgcattg cgtgatatca ccgttgacga
     7201 taaccgtcac gttctccccg aggcgggtta aaaacatttt catttgcgcg gcagtcacat
     7261 tctgcgcctc gtcaagaatg acgactgcat tttcaaaggt acgtccacgc atataggcga
     7321 acggcgcaat ttccaccttc cctatttccg gtcgcaggca gtactgcata aaggaagccc
     7381 ctaagcgccg gaccagcacg tcgtagaccg ggcgaaaata gggagcaaac ttttctgcga
     7441 tatctccagg taagaagcca agatcttcat cggcttgcag aactggacgg gtgacgataa
     7501 tcctgtcgac atccttatgt atcagggcct ctgccgcttt tgctgcgctg atccaggttt
     7561 ttccgccccc ggcttcgccc gtggcgaata tcagctgctt actctcaata gccttcagat
     7621 agtgcaattg cgcttcattt cgcgcgagga tgggcgaagt atcgcgactg tcgcgggcca
     7681 taccaatggc ttctacgccg cccatctgca caagcgaggt gaccgattct tcttcacgct
     7741 gcttatggct gcgcgaatcc cgtctcagca cacgttttgc ctcgcgacga gctttgatca
     7801 ctgctttttg tcttcccata gagagcacct tgagttgttt gtattcatca cacgcgccgt
     7861 tggcagcgcg attatgcgca cgaacatcag agggttggct tccttgtaag ccatagtttg
     7921 cttttggata aaatgccgaa aaacggctac gcgcaccgtt tacggcgtcg gtaacacatg
     7981 aaaagaaagg atgaggttga aaatgcaaag tgacgagatg actaccggag gagaaaactc
     8041 cgcgagtggt ggcgcgttga ttatctaaaa catgtccagt acaggacgtt accatccgcg
     8101 atctccatag tgactgacta tcactgccgg gaacttccgc tgctacttaa taagtacaac
     8161 agatctcgca tttattgcaa caatatattt acttatattt aactataaaa cactatttca
     8221 gtgacattag tttctactgg aaagatgaca gagtgatgac agtgatgaaa aaagctgtgt
     8281 gctttcagca ggatttgcat caggcatcag tgcacgatcg ccggatgcgg cgtgaacgcc
     8341 ttatccagcc taccaaaccc gcactgattg taggcatgat aagacgcgca agcgtcgcat
     8401 caggcatctg cgcacaaatg ccttatccgc ctaaaaacat taaacccgca ataacgcgct
     8461 tccgagataa tcgttcgcgt ccttcactcc cggcagcgaa aaaaaataac cgccgccgat
     8521 aggtttaacg tattcctcca gcgcttcgcc attgagcctt ttttgtactg tcaggaagcc
     8581 tttttccaga tcgtgttggt agcagacaaa cagcaacccc atatccagtt gtccggagtt
     8641 ggtgaccccc aacgaataac tgtagccgcg acgcagcatc aggctggact cactctccgc
     8701 cgtgcgggga ttcgccagcc ggatatggct gtccagcgca atcaccttcc cttccgggtc
     8761 gctggcgtaa tcaggcacat catgctcatg ctgcattccc agcggcgcac cggtttgctt
     8821 atcacggcca aaaatcgtct gctgttcttt cagcggcgtt ctgtcccaaa attccactcg
     8881 aaactgaatc aagcgtactg cctgatagct gccaccgatt gtccacgcag gctcctgctg
     8941 atctgccgtt acccacacca ctttttgcat caacttatca ttctggctat cgggattggc
     9001 agtgccgtct ttgaaaccca gcaaattaat cggcgtctct ttgcctttac tacgcgacac
     9061 gtgatcggaa ataaaccctt cccgcttcca gcgcacactg agcaaatccg gcgtgtgttt
     9121 gatgatatcg cgcagcgcat ggataaccgt gtcctgggtg ttggcgcaaa tctgtagcaa
     9181 cacatcacca tgacataacg ccgcatccag cgagtcgttg gggaaacgcg tcatcttctg
     9241 cagctttttt ggcatctgtg gcgcaaggcc aaagcgctca tcaaacaatg agtgacccac
     9301 cgataacgtg atggtgagat tatcgggcgc aatgtagccg ccaagaatgc cggaatcgag
     9361 tggtggcagg cgcggatttg gcgtttctgg tgctgctccg ccctgagtca gaaaagcaaa
     9421 acgctgagtc aacaagcgaa acaaccgctc aagatcggct ttatcgctgg caagcacatc
     9481 aaacgccacc agcatcattg cggcctgttg tggcgtcagg atccctgcct gatgctcacc
     9541 ataaaacggc tgtttctcat tgcgtgcatc cggtgaaagc gtgcccggtg cactttgcgt
     9601 tttttgtgca tgagcgaccg gacaacttcc cgccagcgcc agcgcaccaa tccctttcag
     9661 taaacgtcgg cgtgacggtt cattcacgcc gttttcatct ttatactgca taacgcttaa
     9721 tccagtccca gcacaccgcg aagttgcgcc agatcttccg ccagcgcagt aatcggtcct
     9781 ttcagtgcat tccggtcggc atcggtcaat ttgtcgtagg tttcaaaacc gtctttagta
     9841 cggtatttcg ccagaatggt atcgaccttt ttaaagttgg catcgacttt tgccagcagt
     9901 tccgggttgg ctttttgcag ttgtggacgc agcaaatcga caattttctg cgagccttca
     9961 acgttagcct ggaaatccca cagatcggtg tggctgtagc gatcttcttc accgctaatt
    10021 ttgctggctg ccacttcctc aatcagtccg gctgcgccgc cgaccacttt tgaaggtggg
    10081 aaagccagtt cactgatgcg tttttgcaaa tcgaccacat ctgtataaag ctgctcagcg
    10141 tactgatcca tccctttggt ggtgttgtcg ccaaacaatg ctttttccag acggtggaaa
    10201 ccagtgaatt ttgggtcggc ggctttttgc tcgtaatcat cttcacgggc gtcaatgctg
    10261 ccatccagat cggagaacag ttcagcaatc ggttcaatgc gctcatagtg ctggcgcgtc
    10321 ggcgcataca gtgctttcgc tttttcgata tcgcctgctt taatcgcgtc ggtaaaggct
    10381 ttggtgtcgg tcaccagctg cgtggtttcc gccatgacat acgctttata tgcagtaatt
    10441 gcaccaccaa gacttaacag cgcatcactt tgcgccgcat ccgccgttgc ctcacctttg
    10501 acgatcaact tccctttcgg gttagtcagc agaccgcagg tcatatcgta ttcgccaggc
    10561 tgtaaattcg ccgtcatttt ctggctaaaa ccaggggcga tattttcccg ctcttccacc
    10621 accatcacgc ctttgaggat ctcccactcc agcgccttct ggctatggtt ctgaataatg
    10681 aactgtgttt tcccggcgtt aaccgtaatg gtcatcggtt cgcactgctt atccgtcacg
    10741 gtcactttga cctgcggcac atcagcggcg ttagccataa aagcagaaga aaacagcgca
    10801 gccacgctca actgcaatgc gttacggcgg aagttaatgg tcatgacgag actatccctt
    10861 taaagtatgt tgtaactaag caagagtttg cgtcgtattt gttacgcgga gcgagacgct
    10921 gtcgcccctg cgcgtggtgg cagagcaaat gccaccagcg ccgggatgag ataaataaac
    10981 cagacggcga cttcgctgac gctcggtgct tcctgatagc caaaaatccc ttccatcagc
    11041 gtgccaaaca gcgagtgagt tgagagcact gcgctcatat cgaaggcgat ttcctgaaag
    11101 tggttccaca atccggcttc atgaaatgcg cgaatggcac cagcagccag ccctgcggcg
    11161 acgaagagaa taaacaggct ggtccattta aaaaatgcac caagattgag gcgaataccg
    11221 ccccagtaga gcaggaagcc tagcaccacg gcagtagcaa gaccgagcat tgcacccagc
    11281 ggcggccaga tcccgacatc ttgttgaaat gccgccagca ggaaaaagac cgactccagc
    11341 ccttcccttg caacggcaaa aaagaccatc atcaccagcg cccagccatg atgatttcca
    11401 cgctgcaatg cgctatcgac tgcctgctcc agttgtactt tgacgttgcg cgacactttg
    11461 cgcatccaga aaaccatcca ggtaaggatc accacggcga tcaccgccac gataccttca
    11521 aacagttcct gttctttttg cggaaattcg ccggtggttt cgttaatgaa gatacccaag
    11581 cccaggcaca acgcagcggc aagcaacacg ccaatccaca tcacgccaat ccatcggcct
    11641 cgctgggtac gcttaagata gctggcaatc aaactgacaa tcagcgcggc ttcaagtcct
    11701 tcgcgcaaca taatgagaaa cggaacaaac atgccaccca cccttaaacg tttttctgag
    11761 tcaatccatg caaagaaaag taaatcgcag tgatagcgat tatcattacg gcaaagaaaa
    11821 atacaagcgg aatgtttata aaatgatgtt ggaatgtaaa caaagagagg taataccagg
    11881 ggatataaac gattattcca ggtacaaaga tgggattgtt tacaacgagc catgtgttct
    11941 tgccagatgc cgcgtaaacg ccttatccag ccgacaaaac tgtccagact caatgcatca
    12001 cagcttccat gtaaaactga taagcgggtg tatcaagcaa tttcgtccat ccgcgattat
    12061 caaatctaac tttttgcccg gaacataata acagcgcggg tgttggtggc gtcaaaatcg
    12121 ctgaaacgtt ccccgaaggc cggggcagcc cacatggatg tgggctgagg gcgcgtctta
//
LOCUS       DAJTZL010000125        12037 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.125, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000125 DAJTZL010000000
VERSION     DAJTZL010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12037
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.125"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            9..323
                     /locus_tag="OO544_002978"
     CDS             9..323
                     /locus_tag="OO544_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR6768512.1"
                     /translation="MLPPLFIMLAYLNLRAKLDHLPRDFRMGSRRTGIIVVSMLIAIF
                     AVGFVASTFPTGANILTIIFYNVGGIVIFLGFAWWKYSKYIKGLTAEERHIEATPASN
                     VD"
     gene            457..1534
                     /gene="ygjJ"
                     /locus_tag="OO544_002979"
                     /pseudo
     CDS             457..1534
                     /gene="ygjJ"
                     /locus_tag="OO544_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417550.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
     gene            1551..3902
                     /gene="ygjK"
                     /locus_tag="OO544_002980"
     CDS             1551..3902
                     /gene="ygjK"
                     /locus_tag="OO544_002980"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HCR6768513.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVDFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKITSNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWLSPGGAVKFNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGDKEETQSGLNNYARVVEKGLYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWLPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLSMLYNDFFRKQ"
     gene            4128..6146
                     /gene="fadH"
                     /locus_tag="OO544_002981"
     CDS             4128..6146
                     /gene="fadH"
                     /locus_tag="OO544_002981"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HCR6768514.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFAHCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRAVRERVGNDFIIIYRLSM
                     LDLVEDGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYHRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(6191..6607)
                     /gene="higA"
                     /locus_tag="OO544_002982"
     CDS             complement(6191..6607)
                     /gene="higA"
                     /locus_tag="OO544_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HCR6768515.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLADLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(6604..6918)
                     /gene="higB"
                     /locus_tag="OO544_002983"
     CDS             complement(6604..6918)
                     /gene="higB"
                     /locus_tag="OO544_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HCR6768516.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            complement(7203..8339)
                     /gene="rlmG"
                     /locus_tag="OO544_002984"
     CDS             complement(7203..8339)
                     /gene="rlmG"
                     /locus_tag="OO544_002984"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HCR6768517.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            8424..8927
                     /locus_tag="OO544_002985"
     CDS             8424..8927
                     /locus_tag="OO544_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HCR6768518.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPITKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            9004..9687
                     /locus_tag="OO544_002986"
     CDS             9004..9687
                     /locus_tag="OO544_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HCR6768519.1"
                     /translation="MQPRLLLRICFSRRTLKITCLLLLVAGATIFIADRVMVNASKQL
                     TWSDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHVGKVKWLLVSGDNGRKNYDE
                     ASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRAIWL
                     AKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMIGPF
                     SEHGCPAKE"
     gene            9766..10752
                     /locus_tag="OO544_002987"
     CDS             9766..10752
                     /locus_tag="OO544_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR6768520.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNALHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTASLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLESEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL"
     gene            11035..12000
                     /gene="alx"
                     /locus_tag="OO544_002988"
     CDS             11035..12000
                     /gene="alx"
                     /locus_tag="OO544_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HCR6768521.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
BASE COUNT         2861 a         3094 c         3225 g         2857 t
ORIGIN      
        1 cagcgtccat gcttccgccg ttattcatca tgctggctta cctgaattta cgcgccaaat
       61 tagatcacct gccacgcgat ttccgtatgg gctcccgccg caccggtatt atcgttgttt
      121 caatgctgat tgcgatattt gccgtagggt ttgtcgcttc gacattcccg actggcgcga
      181 atattctgac catcattttt tataacgtcg gtggtattgt tatcttcctt ggctttgcgt
      241 ggtggaaata cagtaaatat ataaagggat taacggctga agagcgccat attgaagcga
      301 cgccagccag caatgttgat taagcagaat aataaaaaat gagcaggcat tttccctctt
      361 cctggatgag gagagggaaa atagtttctg cctttatatt tttataatta caacgataaa
      421 aggctgtact ttttctttag ctcatggatt aacacaatga aattaatcac tgcaccatgc
      481 agagcattac ttgctctgcc gttttgctac gccttttctg cggcaggaga agaagcacgt
      541 ccggcagaac atgacgacac aaaaacaccc gcaattacct cgacatcttc tccttcattt
      601 cgtttttacg gcgaattagg gattaggggg tggtggatat atggatttag agggtgagaa
      661 taaacataaa tacagcgacg gtacctatat tgaaggtggc ctggagatga agtacggctc
      721 ctggttcggc ctgatttacg gcgaaggctg gaccgtgcag gccgaccacg acggcaatgc
      781 ctgggtgcca gaccatagct ggggtggttt cgagggcgga attaaccgtt tctatggcgg
      841 ttatcgtacc aatgatggca ccgaaatcat gctcagtctg cgtcaggatt cctcgctgga
      901 tgacctgcaa tggtggggcg atttcacccc cgatctgggc tacgtcattc ccaatacccg
      961 cgacattatg actgcgctga aggtacagaa cttaagcggc aactttcgtt atagcgtcac
     1021 cgcgactcct gccggacatc atgatgaaag caaagcctgg ctacattttg gcaaatacga
     1081 tcgctatgac gacaaataca cctatccggc aatgatgaac ggttacatcc agtatgacct
     1141 tgccgaaggc atcacctgga tgaacggtct ggaaatcacc gacggcacag gacagctcta
     1201 tctcacgggc ctgctaactc ctaactttgc cgctcgcgcc tggcaccata ccggacgcgc
     1261 cgacgggctg gacgtaccgg gaagtgaaag tgggatgatg gtgagcgcca tgtatgaagc
     1321 gttaaagggc gtttatctct ccaccgctta cacctacgcc aaacatcgcc ctgaccacgc
     1381 tgacgatgaa accacctctt tcatgcagtt tggtatctgg tacgaatacg gcggcggacg
     1441 tttcgccacg gcttttgata gccgcttcta catgaaaaat gcctctcacg atcccagcga
     1501 ccaaatcttc ctgatgcaat atttctactg gtaataagga ctgtaatttc atgaaaataa
     1561 aaactatttt aacgccagta acctgcgctc tgctgataag tttttccgcc catgccgcta
     1621 acgccgacaa ttataaaaac gtgattaacc gtactggcgc gccgcagtac atgaaggatt
     1681 acgattacga cgatcaccag cgttttaatc cgtttttcga tctcggagcc tggcatggtc
     1741 atctgttgcc agacggccct aacaccatgg gcggctttcc gggcgttgcg ctgctgacgg
     1801 aagagtacat caactttatg gccagcaatt tcgaccgcct gaccgtctgg caggacggca
     1861 agaaagtcga ctttacgctg gaggcataca gtattcccgg tgcgctggtg caaaaactga
     1921 cagcaaaaga tgtgcaagtc gaaatgactc tgcgcttcgc cacgccgcgc acgtcactac
     1981 tggaaaccaa aatcaccagc aataaaccgc tggatctggt gtgggacggc gaactgctgg
     2041 aaaaactgga agcgaaagaa gggaaaccgc tttccgataa aaccattgct ggcgaatacc
     2101 ctgactatca gcgcaaaatc agcgccaccc gtgatggcct gaaagtcacc tttggcaaag
     2161 tgcgcgccac ctgggatctg ctgacctccg gcgaatcgga atatcaggtg cataaatccc
     2221 tgccggtgca gactgaaatc aacggtaatc gctttaccag taaggcgcat atcaacggtt
     2281 cgaccacgct ctacaccacc tattcccatc tgctgaccgc tcaggaagtt agcaaagagc
     2341 aaatgcagat ccgcgatatt ctggcgcgtc cggcgtttta tctcaccgcc tcgcagcaac
     2401 gctgggaaga atacctgaag aaagggttaa ccaatccgga tgcgacgccg gaacagacgc
     2461 gcgtcgcggt gaaagcgatc gaaacgctca acggtaactg gctctcaccg ggcggtgcgg
     2521 tgaaatttaa caccgttaca ccgtcggtga ccgggcgctg gttctccggc aatcagacct
     2581 ggccgtggga tacctggaag caggcgtttg cgatggcgca tttcaatccg gacatcgcca
     2641 aagagaatat ccgcgcggtc ttctcctggc agatccagcc tggcgatagc gtgcgtccgc
     2701 aggatgtggg ctttgtcccc gacctgatag cgtggaacct tagccccgag cgtggtggcg
     2761 atggcggcaa ctggaacgaa cgtaatacca aacccagcct tgccgcctgg tcggtgatgg
     2821 aagtgtacaa cgtcacccag gataaagcct ggttggcaga gatgtacccg aaactggtgg
     2881 cctatcacga ctggtggtta cgtaaccgcg atcacaacgg caacggcgtg ccggaatatg
     2941 gcgcgacccg cgacaaagcc cacaacactg agagcggcga gatgctgttt acggtgaaaa
     3001 aaggcgataa agaagagacg cagtctgggc tgaacaacta cgcccgcgtg gtggagaaag
     3061 gcctgtacga cagtctggaa attccggcac aggttgctgc atcgtgggaa tcggggcgtg
     3121 atgatgccgc cgtctttggg tttatcgaca aagaacagct ggataaatat gtcgctaatg
     3181 gcggcaaacg tagcgactgg acggtgaaat tcgccgaaaa ccgcagtcag gacggaacgt
     3241 tgctgggcta ctcgctattg caggagtcgg tggatcaggc cagctatatg tacagcgata
     3301 accattatct ggcggagatg gcaaccatcc tcggtaagcc agaagaggcc aaacgctatc
     3361 gccagttggc acagcagctc gcggactaca tcaacacctg tatgttcgac ccgactaccc
     3421 agttctacta tgacgtacgt attgaagata aaccactggc gaacggctgc gcgggcaaac
     3481 cgattgttga gcgcggtaaa gggccggaag gctggttgcc gctgtttaac ggtgcggcaa
     3541 cgcaggccaa cgccgacgcg gtggtgaagg tgatgctcga tcctaaagag ttcaacacct
     3601 ttgtcccgct gggaacggcg gcgttaacca atccggcttt tggcgctgat atctactggc
     3661 gtgggcgcgt atgggtggat cagttctggt ttggtctgaa agggatggag cgttacggtt
     3721 atcgtgatga tgccctgaag ctggcggata cgttcttccg gcacgccaaa ggattaaccg
     3781 ccgatggccc aattcaggag aattacaacc cgctgactgg cgcacagcaa ggcgcaccaa
     3841 atttctcctg gagtgccgct catttgtcta tgttgtataa cgattttttc cgtaagcagt
     3901 aatttgcaac ggctggcgga tgcggcgtga acgccttatc cgccctacat gtgcaatccc
     3961 ccaggccgga taaaacgcgg caagcgttgc atccggcgtt accaccgggc gtattctttt
     4021 tgaatcccat cacaaacccc gcactcccct tttccctttt ctccatcgac ggctaaatta
     4081 gaactcatcc gaccacataa caattatttt acatactgga caattttatg agctatccgt
     4141 cgctgttcgc cccgctggat ttaggtttta ccacgttaaa aaaccgcgtg ttgatgggct
     4201 caatgcacac cgggctggag gaatacccgg acggtgccga gcggctggca gcgttttatg
     4261 ccgaacgcgc ccgtcacggc gtggcgctga ttgtcagcgg cggcattgca ccagatttaa
     4321 caggcgttgg catggaaggt ggcgcaatgc tcaacgacgc cagccagatc ccacaccatc
     4381 gcaccattac cgaagcggta catcaggaag gcggcaaaat agccctgcaa attttgcata
     4441 ccgggcgcta cagctaccaa ccgcatctgg tcgctccctc cgcattgcag gcccccatca
     4501 accgtttcgt tcctcacgaa ctcacccatg aagagatcct gcaactgatc gacgatttcg
     4561 cccactgcgc gcaactggca cgggaggcag gatacgacgg tgtagaggta atggggtccg
     4621 aagggtattt gatcaacgaa tttctgacgc tgcgcaccaa tcagcgtagt gaccagtggg
     4681 gcggcgatta ccgcaaccgg atgcgatttg ccgtagaagt agtgcgtgcg gtgcgcgaac
     4741 gcgtcggcaa cgacttcatt attatctacc gactgtcgat gctcgacctg gtcgaagacg
     4801 gcgggacttt tgccgaaacg gtagagctgg cgcaggccat tgaagcggcg ggcgcgacca
     4861 ttatcaacac cggcattggc tggcatgaag cacgtattcc gaccattgcc acgcccgtgc
     4921 cgcgcggcgc atttagctgg gtcacgcgca aattgaaagg ccacgtctcg ctgccgctgg
     4981 taaccaccaa ccggattaac gatccgcagg ttgccgacga tattctctcg cgcggcgatg
     5041 ccgatatggt atcgatggca cgaccgtttc ttgctgatgc ggaactgctg tcaaaagcgc
     5101 aatcggggcg agccgatgag atcaacacct gcattggctg caatcaggcc tgcctcgatc
     5161 agatcttcgt tggcaaagtc acctcgtgcc tggtgaatcc tcgcgcctgc cacgaaacca
     5221 aaatgccaat ccttcctgcc gtgcagaaaa aaaatctggc ggtggtcggt gcgggacctg
     5281 ctgggctggc gtttgccatt aacgcggcgg cgcgtgggca tcaggtaaca ttgtttgatg
     5341 cccacagcga gatcggcggg cagtttaata tcgccaaaca gatccccggc aaagaggagt
     5401 tttacgaaac gctgcgctat taccaccgga tgatcgaagt gacgggcgtg acgctgaaac
     5461 tcaatcacac cgtgacggcg gatcagttac aggcgttcga tgaaacgatc ctcgccagtg
     5521 ggatcgtgcc gcgcgttccg cctatcgacg ggatcgatca tccgaaggta ttgagttatc
     5581 tcgatgtact gcgcgacaaa gcgccggtag gcaacaaggt tgccatcatc ggttgtggcg
     5641 ggattggttt tgatacggcg atgtatttaa gtcagccggg cgaatccacc agccagaata
     5701 tcgccaggtt ctgtaatgag tgggggatcg acagcagcct acaacaggct ggtggcttaa
     5761 gcccgcaggg aatgcagatc ccgcgtagcc cacggcaaat tgtgatgctc cagcgcaaag
     5821 ccagcaaacc gggacagggg ttaggcaaaa ccaccggctg gatccatcgc accaccctgc
     5881 tctcgcgggg tgtgaaaatg atcccaggcg taagttatca gaagattgac gatgacgggc
     5941 tgcatgtggt gatcaacggc gaaacgcagg tattagcagt ggacaatgtg gtgatctgcg
     6001 cagggcaaga gccaaaccgc gcgctggcgc aaccgctgat tgatagcggg aaaactgtgc
     6061 atttaattgg cggctgcgat gtggctatgg agctggacgc acgacgggca attgcccagg
     6121 gaacacggct ggcgctggag atttaaatcg ttattttaag cgccggatgc aacgcatccg
     6181 gcacgtcgcg ttaatcaata aacaaggcgg gagaaatgcc gaatcgcgtt gccaattttt
     6241 tagcgtgttc cagcgttaat ttcctcttcc cgctcaaaac gcgtgacacc atagatttac
     6301 tgccaatttc cggcagatcg gcaagggtta aaccatattg atccataagg gtacgaatca
     6361 cggctatacc gccaggcatg gcttgagcca tggcattaaa ttccgcaaat tcgggcgctg
     6421 attcttccca cgcggttatt ttggcacaca ccagatccag caaggggttt tcaggatcgt
     6481 tgagcagcag atgatctacc agttccagcg cctgggtgta ttgttcctcg ttctgaatac
     6541 ccgccagaaa aggtgccacc gcagttagct tttctcctgc ttgcaagatg tcggcaatcg
     6601 caatcatttt ttccccttag tacgatgaac agcggtaaag aaatcgtatt ctttatgcgt
     6661 cataacttca cgtatgtagc acttttgcga ttcaaaaaag accattgcta caacacgtaa
     6721 ttcattgccc ccaacattga aaacataatg cttatccaga tatttgaagt tatccagaga
     6781 tgggaatact gcttttaatg actcaggttt tttgaaatat cccttagcaa tcgtgttccc
     6841 cagagccacc aactccgttt tatgttgcgg gtatttttcc gcagcatctt tcaatgcttt
     6901 ttgagttatc aggtgcattc ttcatcacgt ccgttgacaa attggcaata tgataacatc
     6961 cgttgacaga ttggcaacgg atgaatttat ctgtgtgacg gaagaataaa cgtagaaaaa
     7021 cgctaaacaa gagaatgtag aggatggtga gagggatttt ttgttttatg gaagcgcgat
     7081 tccagaactg ggcagacatt gccggatgcg acgcttgccg cgtcttatcc ggcctgggag
     7141 taacgtgctg aacgtaggcc ggataaggcg ttaacgccgc atccggcagg taactcccaa
     7201 tcttaccgac gacgccccag cttcactgct ttcagcacca caaatttatt attcgtggcg
     7261 atggtggtgc agttgccgaa aatcttcttc agtttatgga agtaatccag gtgacggttg
     7321 gcaacaatat acagctcgcc gttgattttc aggcagcggc gggcatggtg gaacatctcc
     7381 caggcgacgt tatcggtcag cgcatgttgt tggtgaaacg gcgggttgca gagcacagca
     7441 ttaaagcgga aaggctccac gccggagagc gcgttgttga tcataaactc gctgcgatct
     7501 aacgcctctg gcatgttggt ttcaacgttc aaacggctgg aagcaaccgc catcggcgat
     7561 tcatcgacaa acaccacttt cgcctgaggg tttttatcaa gcagcgtcag accaataacg
     7621 ccattaccgc aaccgagatc gacaatctca ccttcgagat tctctggcag atgttgcata
     7681 aagaagcgtg cgccaatatc cagcccggtg cgggagaaga cattcgcatg gttgtggata
     7741 gtccagtcag taccttccag cttccagcta acggtctgcg gcgcatcggc cagcggcggt
     7801 tcattgaaag tgcaattaat caggcgcgct tttttccatg ccagcgtagt ggtggtcggg
     7861 ccgagcactt tctcgaacag ctccagcgtg gaagtgtgaa tgtcacgggc tttggcaccg
     7921 gcaataatac gtgtatccga cgtgaccact ttgcgcagcg cacgcagttg ctgttccagc
     7981 aatgccagtg ttttcggcac tttgatcagc accacacccg gctgttgcgg gtagtcggcg
     8041 gtgctgtcga gaaacttcac gctcgactca tcaatcccgt tgaggcgtaa attctcgcgc
     8101 gtcgccagtt cactgatgta tgagtcgcca atgctgtacg gcttatgttc cgccagcgcg
     8161 cagcttaacg caccaaaggc atcattcagg atcaacaccg ggccgcggat ttctgtgtcg
     8221 tccaactgtt gcagcaaata ttcatccgcc gcttcccacg cctgtagcgg gttaacgtca
     8281 tccgtcgccg gaaaacgttg tagtgtcagt gaacggaaac cgttgtctaa gtggctcatc
     8341 ggccctcctg aatgataaaa tttcgacgtt gcccctgaaa agggtgcgtg agtataccct
     8401 ttttctcttt ttcgtgggcg gttatgagca atcttactta tcttcagggt tatcccgagc
     8461 agctactttc ccaggtgcga acgctgatta acgaacagcg tctgggcgat gtgctggcaa
     8521 aacgctatcc gggaacacac gactacgcca ccgataaagc cctctggcaa tatacccagg
     8581 atctaaaaaa tcagtttctg cgcaatgccc cgccgatcac taaagtgatg tatgacaata
     8641 agatccacgt actgaaaaac gcgctcggat tacataccgc tgtttctcgt gtgcagggcg
     8701 gcaagctgaa agcaaaagca gagatccgcg tcgctactgt gtttcgcaac gcgccggaac
     8761 cgtttttacg catgatcgtg gtgcacgaac tggcgcacct gaaggagaaa gagcataaca
     8821 aagcgtttta ccagttgtgc tgccatatgg agccgcagta ccaccagctt gagttcgaca
     8881 cccggctgtg gctaacgcag ttgtcgcttg ggcaggacaa aatctgaaaa tgatgtcagg
     8941 cgttatcttt tctttcttta gcgcctaaaa tcgacctccc ccctttcgtt aaggataacg
     9001 accatgcagc cccgccttct tctccgtatt tgtttttctc gccggactct aaagattacc
     9061 tgcttactgt tacttgtggc aggagcgacc atttttatcg ccgatcgcgt aatggtcaac
     9121 gccagcaagc agctgacctg gagcgatgtc aacgcagttc cggcgcgtaa cgtgggttta
     9181 ttgctggggg cgaggccggg taatcgctac tttacccgac gtattgatac cgcagcagcg
     9241 ttgtaccacg tcggaaaagt gaaatggctg ttagtcagtg gcgataacgg acgtaaaaat
     9301 tacgatgaag cctcaggtat gcagcaggct ttgatcgcta aaggcgtacc ggcgaaagtg
     9361 atcttctgcg actatgccgg attctcaacg cttgattcag tggtgcgtgc caaaaaggtg
     9421 tttggggaaa accatatcac cattatctcg caagaatttc ataaccaacg cgccatctgg
     9481 ctggcaaaac agtacggcat cgacgctatc gggtttaacg cgccagacct taatatgaag
     9541 cacggttttt atactcaact gcgggagaaa ctcgctcgtg tcagcgcggt gattgacgcc
     9601 aaaatcctcc atcgccagcc gaaatatctc gggccatcgg tcatgattgg gccgtttagt
     9661 gagcatggct gtccggcgaa agaataatgc gtatctgcgc acgtcgaaga tgaaaaaggc
     9721 gtgctacatt gacgacagaa tccctttatg gagtatccac gcgttatgat acgtttcgct
     9781 gtgattggta cgaactggat cactcgccag ttcgtcgagg ccgcccatga gagcggtaaa
     9841 tacaagttaa ccgccgtata ttcccgcagc cttgaacagg cccagcactt cgccaatgat
     9901 ttttctgtcg agcatctgtt tacctcgctg gaagcgatgg cggaaagcga tgccattgac
     9961 gcggtgtata ttgccagccc gaatgccctg cacttttccc agacacaact tttccttagc
    10021 cataaaattc atgtgatttg cgagaaacca ctggcgtcga atctggcgga agtggatgcc
    10081 gccattgcct gtgcgcggga aaatcaggtg gtgctgtttg aggcatttaa aaccgccagc
    10141 ctgccgaact ttcatttgct gcgccaggcg ctgccgaaag tcggcaaatt gcgtaaagtc
    10201 tttttcaact attgccagta ttcctcgcgg tatcaacgtt acctggatgg tgagaatccc
    10261 aacaccttta atccggcatt ctctaacggt tcaattatgg atatcggctt ttactgcctg
    10321 gcgtcggcgg tggcattatt cggtgaaccg aaaagcgtgc aggcaaccgc cagtttgttg
    10381 gcaagcggcg tagatgccca cggcgtggtg gtgatggatt acggtgattt cagcgttacg
    10441 ttgcagcact caaaggtcag tgattctgtc ctggaaagcg agattcaggg cgaagcggga
    10501 tcgctggtga ttgaaaaact gtctgaatgc cagaaagtgt gcttcgtgcc gcgtggcagc
    10561 cagatgcagg atctcaccca gccgcagcat attaatacca tgctctacga agcagagctg
    10621 tttgctaccc tggtggatga gcatctggtg gatcatccag ggctggcggt cagtcgcatc
    10681 accgccaaac tgctgactga gatccgccgc cagactgggg tgatttttcc ggcagatagc
    10741 gtaaaactat aattgccaaa gtaaaacagt gtaaaaggta tgtaacagac cattgactgg
    10801 ctgaatggtc tgtcatactt tgttacctgc aaaggggagt aacttcattg ccggtcgatc
    10861 gtcattacga tgtgtgaaaa accacatccg gtcaccgggc aacccgaaag gaatacgcag
    10921 acgtattcct tttttgttgt aagtgagacc ttgccggaag gcgaggtcta tgcataaaaa
    10981 gcagcggctg acgtcttccg acgttggccg tttttttatg tgtaaggaac ttctatgaat
    11041 actgtcggca cgccgttgct atggggcgga ttcgctgttg ttgtcgccat tatgctggct
    11101 atcgacctgt tgttgcaggg gcgtcgtggg gcacatgcca tgaccatgaa acaggctgcg
    11161 gcctggtcgc tggtctgggt gacgctgtcg ttactgttta acgccgcttt ctggtggtat
    11221 ctggtgcaaa ctgaaggtcg cgccgttgcc gatccacagg cactggcctt tctcacgggt
    11281 tatctgattg agaaatcgct ggcggtcgat aacgtctttg tctggctgat gttgttcagc
    11341 tatttctctg ttccggcggc attacaacgc cgcgtgctgg tgtatggcgt gctcggggcg
    11401 attgttctgc gtaccatcat gatcttcact ggcagctggc taatttcgca gttcgactgg
    11461 atcctgtata tcttcggtgc cttcctgctg tttaccggcg tgaagatggc gctcgcccat
    11521 gaagatgaat caggcattgg cgacaagccg ctggtgcgct ggctacgcgg tcatttgcgc
    11581 atgaccgaca ccatcgacaa cgagcatttc tttgtgcgta agaatggatt gctgtacgcc
    11641 acaccgctga tgctggtact gattctggtg gaattgagcg acgtgatttt cgccgtggat
    11701 agcattccgg ctatcttcgc cgtgaccact gacccgttca ttgtgctgac ctcaaacctg
    11761 tttgcgatcc tcggcctgcg tgcgatgtat ttcctgctgg cgggcgtagc agagcgtttc
    11821 tcgatgctca aatatggcct ggcggtgatt ctggtgttta tcggtatcaa gatgctgatt
    11881 gtcgacttct accatattcc aatcgccgtc tcgctgggcg tggtgtttgg cattctggtg
    11941 atgacgttta ttatcaacgc ctgggtgaat tatcggcatg ataagcagcg gggagaatga
    12001 ttcgctgtat attaagccat tcctagagat aactcat
//
LOCUS       DAJTZL010000126        11877 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.126, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000126 DAJTZL010000000
VERSION     DAJTZL010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11877
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.126"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            486..1157
                     /locus_tag="OO544_002989"
     CDS             486..1157
                     /locus_tag="OO544_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HCR6768522.1"
                     /translation="MDKVLDSALLSSANKRKGILAIGAHPDDIELGCGASLARLAQKG
                     IYIAAVVMTTGNSGTDGIIDRHEESRNALKILGCNQTIHLNFADTRAHLQLNDMISAL
                     EDIIKNQIPSDVEIMRVYTMHDADRHQDHLAVYQASMVACRTIPQILGYETPSTWLSF
                     MPQVFESVKEEYFTVKLAALKKHKSQERRDYMRHDRLRAVAQFRGQQVNSDLGEGFVI
                     HKMIL"
     gene            1167..2363
                     /locus_tag="OO544_002990"
                     /pseudo
     CDS             1167..2363
                     /locus_tag="OO544_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414897.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
     gene            2363..2923
                     /locus_tag="OO544_002991"
     CDS             2363..2923
                     /locus_tag="OO544_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCR6768523.1"
                     /translation="MLKRKIIQIADQVIIDETAGEVVIGANTRICHGAVIQGPVVIGA
                     NCLIGNYAFIRPGTIISNGVKIGFATEIKNAVIEAEATIGPQCYIADSVVANQAYLGA
                     QVRTSNHRLDEQPVSVRTPEGIIATGCDKLGCYIGQRSRLGVQVIILPGRIISPNTQL
                     GPRVIVERNLPTGTYSLRQELIRTGD"
     gene            2925..3698
                     /locus_tag="OO544_002992"
     CDS             2925..3698
                     /locus_tag="OO544_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HCR6768524.1"
                     /translation="MIKRTLLAAAIFSALPAYAGLTSITAGYDFTDYSGDHGNRNLAY
                     AELVAKVENATLLFNLSQGRRDYETEHFNATRGQGAVWYKWNNWLTTRTGIAFADNTP
                     VFARQDFRQDINLALLPKTLFTTGYRYTKYYDDVEVDAWQGGVSLYTGPVITSYRYTH
                     YDSSDAGGSYSNMISVRLNDPRGTGYTQLWLSRGTGAYTYDWTPETRYGSMKSVSLQR
                     IQPLTEQLNLGLTAGKVWYDTPTDDYNGLQLAAHLTWKF"
     gene            3886..4161
                     /gene="frmR"
                     /locus_tag="OO544_002993"
     CDS             3886..4161
                     /gene="frmR"
                     /locus_tag="OO544_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HCR6768525.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSLEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            4196..5305
                     /gene="frmA"
                     /locus_tag="OO544_002994"
     CDS             4196..5305
                     /gene="frmA"
                     /locus_tag="OO544_002994"
                     /EC_number="1.1.1.284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HCR6768526.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFDLARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            5398..6231
                     /gene="fghA"
                     /locus_tag="OO544_002995"
     CDS             5398..6231
                     /gene="fghA"
                     /locus_tag="OO544_002995"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HCR6768527.1"
                     /translation="MELIEKHASFGGWQNVYRHYSQSLKCEMNVGVYLPPKAANEKLP
                     VLYWLSGLTCNEQNFITKSGMQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGF
                     YLNATQAPWNEHYKMYDYIRNELPDLVMQHFPATTRKSISGHSMGGLGALVLALRNPD
                     EYVSVSAFSPIVSPSQVPWGQQAFAAYLGENKDAWLDYDPVSLISQGQRVAEIMVDQG
                     LSDDFYAEQLRTPNLEKICQEMNIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(6457..6996)
                     /locus_tag="OO544_002996"
     CDS             complement(6457..6996)
                     /locus_tag="OO544_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HCR6768528.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTDGNLIKKI
                     FVDKLTQAQLINGRLAIARLLVDNNSEGEYTIIPASVADKIAQRDASSIVLHSALSAE
                     EQDEDDPYADFKVPDDLMW"
     gene            complement(7098..8309)
                     /gene="mhpT"
                     /locus_tag="OO544_002997"
     CDS             complement(7098..8309)
                     /gene="mhpT"
                     /locus_tag="OO544_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
                     /protein_id="HCR6768529.1"
                     /translation="MSTRTPSSSSSRLMLTIGLCFLVALMEGLDLQAAGIAAGGIAQA
                     FALDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKRILIGSVALFGLFSLATAIAWD
                     FPSLVFARLMTGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAATLGF
                     AGANLAWQTVFWVGGVVPLILVPLLMRWLPESAVFAGEKQAAPPLRALFAPETATATL
                     LLWLCYFFTLLVVYMLINWLPLLLVEQGFQPSQAAGVMFALQMGAASGTLMLGALMDK
                     LRPVTMSLLIYSGMLASLLALGTVSSFNGMLLAGFVAGLFATGGQSVLYALALLFYSS
                     QIRATGVGTAVAVGRLGAMSGPLLAGKMLALGTGTVGVMAASAPGILVAGLAVFILMS
                     RRSRMQPCADA"
     gene            complement(8596..9609)
                     /gene="dmpG"
                     /locus_tag="OO544_002998"
     CDS             complement(8596..9609)
                     /gene="dmpG"
                     /locus_tag="OO544_002998"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HCR6768530.1"
                     /translation="MNGKKLYISDVTLRDGMHAIRHQYSLENVRQIAKALDDARVDSI
                     EVAHGDGLQGSSFNYGFGAHSDLEWIEAAADVVRHAKIATLLLPGIGTIHDLKNAWQA
                     GARVVRVATHCTEADVSAQHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGA
                     TCIYVVDSGGAMNMSDIRDRFRALKAVLKPETQTGMHAHHNLSLGVANSIAAVEEGCD
                     RIDASLAGMGAGAGNAPLEVFIAAADKLGWQHGTDLYALMDAADDLVRPLQDRPVRVD
                     RETLALGYAGVYSSFLRHCETAAARYGLSAVDILVELGKRRMVGGQEDMIVDVALDLR
                     NNK"
     gene            complement(9606..10556)
                     /gene="mhpF"
                     /locus_tag="OO544_002999"
     CDS             complement(9606..10556)
                     /gene="mhpF"
                     /locus_tag="OO544_002999"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase"
                     /protein_id="HCR6768531.1"
                     /translation="MSKRKVAIIGSGNIGTDLMIKILRHGQHLEMAVMVGIDPQSDGL
                     ARARRMGVATTHEGVIGLMNMPEFADIDIVFDATSAGAHVKNDAALREAKPDIRLIDL
                     TPAAIGPYCVPVVNLEANVDQLNVNMVTCGGQATIPMVAAVSRVARVHYAEIIASIAS
                     KSAGPGTRANIDEFTETTSRAIEVVGGAAKGKAIIVLNPAEPPLMMRDTVYVLSDEAS
                     QDDIEASINEMAEAVQAYVPGYRLKQRVQFEVIPQDKPVNLPGVGQFSGLKTAVWLEV
                     EGAAHYLPAYAGNLDIMTSSALATAEKMAQSLARKAGEAA"
     gene            complement(10553..11362)
                     /gene="mhpD"
                     /locus_tag="OO544_003000"
     CDS             complement(10553..11362)
                     /gene="mhpD"
                     /locus_tag="OO544_003000"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HCR6768532.1"
                     /translation="MTKHTLEQLAADLRRAAEQGEAIAPLRDLIGIDHAEAAYAIQHI
                     NVQYDVVQGRRVVGRKVGLTHPKVQQQLGVDQPDFGTLFADMCYGDNEIIPFSRVLQP
                     RIEAEIALVLNRDLPATDITFDELYNAIEWVLPALEVVGSRIRDWSIQFVDTVADNAS
                     CGVYVIGGPAQRPAGLDLKNCAMKMTRNNEEVSSGRGSECLGHPLNAAVWLARKMASL
                     GEPLRAGDIILTGALGPMVAVNAGDRFEAHIEGIGSVAATFSSAAPKGSLS"
     gene            complement(11372..>11877)
                     /gene="mhpC"
                     /locus_tag="OO544_003001"
     CDS             complement(11372..>11877)
                     /gene="mhpC"
                     /locus_tag="OO544_003001"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414883.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HCR6768533.1"
                     /translation="AFTLNWPERVGKLVLMGGGTGGMSLFTPMPTEGIKRLNQLYRQP
                     TIENLKLMMDIFVFDTSDLTDALFEARLNNMLSRRDHLENFVKSLEANPKQFPDFGPR
                     LAEIKAQTLIVWGRNDRFVPMDAGLRLLSGIAGSELHIFRDCGHWAQWEHADAFNQLV
                     LNFLARP"
BASE COUNT         2912 a         3177 c         2908 g         2880 t
ORIGIN      
        1 aatatgcaat tactccgttc taacaattgg gaaaattact acacttcatt ttagtgatgc
       61 ataaacaacc acattacgag atttattact tcattgtttc tatcaataca tataataaaa
      121 ctattatatt cgcattgtat atatcactgt atcattctca atcactcgca tattacattc
      181 acataatgta tataaacaat acttgttcac agaacatatt ttttaaatgc aattaattga
      241 caaactaaat cacatctaaa tacaatgtat aaaacaaaat aactatacat accaaacata
      301 caaacactta acatatgaat tgcataaaaa cattacccag tcgaacctcc gggtctgaaa
      361 gcccttgctt ttcagttatt agcaacattt aacatttata aattaacatc tggctgcgcc
      421 agagattacg tctctgagtg catcgttgtg cttgtagtat aataaataaa atggagtgtc
      481 attaagtgga taaggtttta gattcagccc tcctttcttc ggcaaataaa agaaagggta
      541 tccttgctat tggcgcacat cctgacgata tagaattagg ctgtggcgca tcgcttgctc
      601 gtcttgcgca aaaaggaatt tatatcgcag cagtggtgat gactaccggc aactctggta
      661 cagatggaat aatcgatcgc catgaagaat cgcgcaacgc cctaaagata ttagggtgca
      721 accaaactat tcatcttaat tttgctgaca cccgcgctca tttacagctc aatgatatga
      781 tttccgccct ggaagacatc attaaaaatc aaattccttc tgatgttgaa atcatgcggg
      841 tatataccat gcatgatgcc gaccgccatc aggatcatct cgctgtttat caagcttcaa
      901 tggttgcctg ccgcactatt ccacaaattc tcggctacga aaccccaagt acctggcttt
      961 catttatgcc tcaggttttt gaatccgtta aagaagaata tttcacagtc aaacttgctg
     1021 cattaaaaaa acataaaagc caggaacgac gtgattatat gcgtcatgat cgcctgcgtg
     1081 cagttgcaca atttcgcggg caacaggtca atagcgatct gggtgaaggc tttgtaattc
     1141 ataaaatgat tctttgagca aattgcatga aaacctggat atttatctgt atgtccatag
     1201 caatgttgct atggttttta agtacgctaa gacgtaaacc cagtcaaaag aaaggctgta
     1261 ttgacgccat tatacctgcg tataacgaag gcccgtgtct ggcgcagtca ctggataatc
     1321 tactgcgtaa cccttatttt tgccgggtaa tttgcgttaa cgacggctcc acggacaata
     1381 ccgaagcggt catggcggaa gtcaaacgca aatggggcga ccgctttgtt gccgtcacgt
     1441 aaaaaaatac cggtaaaggt ggtgcgctga tgaatggcct caattatgcc acctgcgacc
     1501 aggttttttt aagtgatgcc gacacctatg ttccgcccga tcaagacgga atgggctata
     1561 tgctggcaga aattaagcgc ggtgctgatg ccgtaggcgg cattccctct actgcgttga
     1621 aaggcgcggg gctgttaccg cacatccgcg cgaccgtaaa gttgccgatg attgttatga
     1681 agcgcacgct acagcagctc ctgggcggcg caccgtttat tatcagcggt gcctgcggaa
     1741 tgttccgtac tgatgtattg cgtaagttcg gtttctcgga tcgtactaaa gtcgaagacc
     1801 ttgatctcac ctggacatta gtggcaaacg gctaccgtat tcggcaggcg aatcgctgca
     1861 tcgtataccc acaggaatgc aacagtccgc gtgaggagtg gcgtcgctgg cggcgttgga
     1921 ttgtgggcta cgcggtctgt atgcgcctgc ataaaaggct tttatttagc cgcttcggta
     1981 tcttcagtat atttcctatg ctgttggttg tgatttatgg cgttgggatt tatctcacta
     2041 cctggtttaa tgaattcatc accaccgggc cgcatggtgt ggtgttggca atgtttccgc
     2101 ttatctggat cggcgtagtt tgtgttattg gtgcttttag cgcctggttt catcgttgct
     2161 ggttgttggt gcctttagcg ccgctttccg ttgtgtatgt attattagct tatgccatct
     2221 ggattatcta tggacttatt gcctttttta ctggacgcga acctcagcgc gacaaaccca
     2281 cccgctattc cgcactggtg gaagcgtcaa ccgcttattc ccaaccttct gtcacaggaa
     2341 ctgaaaaact atctgaagct taatgttaaa gagaaaaatt attcagattg cagaccaggt
     2401 aattattgat gaaactgcag gtgaagttgt tatcggcgcg aatacccgta tttgtcatgg
     2461 tgccgttatt cagggtccgg tagtgattgg cgcaaactgc ctgataggta attatgcgtt
     2521 tattcgtcct ggcacaataa tcagcaatgg tgtaaaaatt ggttttgcca ccgaaattaa
     2581 aaatgcggtt attgaagcgg aagcaacgat tggtccgcaa tgttatattg ccgactcggt
     2641 agttgcaaac caggcatatt tgggcgcaca agtacgtacc agtaatcatc gtctggatga
     2701 acagcctgtg tctgttcgaa ctccagaggg aattatcgct accggatgcg ataaattagg
     2761 ttgttatatc gggcagcgtt cacgccttgg cgtacaagtt attattttgc ctgggcgaat
     2821 tatttccccg aacacgcaac ttggcccgcg cgtgattgta gaacgtaatt tacctaccgg
     2881 aacttattca ctccgacaag aacttatccg tacaggagat taaaatgata aaacggacgt
     2941 tattagcggc ggccattttt agcgcattgc ccgcttatgc cgggttaact tccattaccg
     3001 cgggctacga ttttaccgat tattctggcg atcatggcaa ccgtaattta gcgtatgctg
     3061 aactggtggc gaaagttgaa aacgcaacgc tgctttttaa tctttcccag gggcgtcgtg
     3121 attatgaaac tgaacatttc aatgccactc gcggtcaggg tgcggtctgg tataaatgga
     3181 ataactggct gacaacccga acgggtattg cctttgcgga taatacgccg gtctttgccc
     3241 gccaggattt tcgtcaggat attaacctgg ccctgttgcc aaaaacgctt ttcacgaccg
     3301 gttatcgcta cactaaatat tacgatgatg tcgaagtcga tgcctggcaa ggcggcgtat
     3361 cactctatac tggcccggtg atcaccagct accgctatac ccattatgac tccagcgatg
     3421 caggtggtag ttatagcaat atgatttccg tgcgtctgaa tgacccgcgc ggcactggtt
     3481 atacgcaact atggctaagc cgcggaacag gcgcttacac ctatgactgg acgccagaaa
     3541 cacgctacgg cagcatgaag agcgtcagtt tgcaacgtat tcaaccgctg actgagcaac
     3601 ttaatctcgg gctgacggca ggtaaagtgt ggtacgacac cccaaccgat gattataacg
     3661 gtctgcaact tgcagcccat ctgacctgga aattctgatt gcttctgccg cccgctatcc
     3721 ggggcggcct tccctgccga ttagccccac tccctttcct cgttgttttc cgaccacatt
     3781 caccggataa attttattct ccagcgttat atactatagg gaggtatgca ttgacatata
     3841 gcatacctcc ctatagtata ttgcgtgcag atgatgaggt gcgaaatgcc cagtactccg
     3901 gaagagaaga aaaaggtcct tactcgagtt cgtcgtattc gggggcagat tgatgctctg
     3961 gaacggtcgc tggagggtga tgccgaatgc cgtgccatac tccaacagat cgctgccgtt
     4021 cggggcgcgg ctaacgggct gatggcagaa gtgcttgaaa gccatatccg ggaaacgttt
     4081 gaccgaaatg actgctacag ccgcgaagtc agccaatccg ttgacgacac tattgaactg
     4141 gttcgagcct atcttaaata gctgaatcta ttaccatatt gaggaagagc gagagatgaa
     4201 atcacgtgct gccgttgcat ttgctcccgg taaaccgctg gaaatcgttg aaattgacgt
     4261 tgcaccaccg aaaaaaggtg aagtgctgat taaagtcacc cataccggcg tttgccatac
     4321 cgacgcattt accctctccg gggatgaccc ggaaggtgta ttcccggtgg ttctcggtca
     4381 cgaaggggcc ggcgttgtgg ttgaagtcgg tgaaggcgta accagcgtca aacctggcga
     4441 ccatgtgatc ccgctttaca ccgcggagtg cggcgagtgt gagttctgtc gttctggcaa
     4501 aactaacctc tgtgttgcgg ttcgcgaaac ccagggtaaa ggcttgatgc cagacggcac
     4561 cacccgtttt tcttacaacg ggcagccgct ttatcactac atggggtgct ctacattcag
     4621 tgaatacacc gtggtcgcgg aagtgtctct ggccaaaatt aatccagaag caaaccatga
     4681 acacgtctgc ctgctgggct gtggcgtgac caccggtatt ggcgcggtgc acaacacagc
     4741 taaagtccag ccaggtgatt ctgttgccgt gtttggtctt ggcgcgattg gtctggcagt
     4801 ggttcagggc gcgcgtcagg cgaaagcggg acggattatc gctatcgata ccaacccgaa
     4861 gaaattcgat ctggcgcgtc gcttcggtgc taccgactgc attaacccga atgactacga
     4921 caaaccgatt aaagatgtcc tgctggatat caacaaatgg ggtatcgacc atacctttga
     4981 atgcatcggt aacgtcaacg tgatgcgtgc ggcgctggaa agtgcgcacc gcggctgggg
     5041 tcagtcggtg atcatcgggg tggcaggttc tggtcaggaa atctccaccc gtccattcca
     5101 gttggtcact ggtcgcgtat ggaaaggttc cgcgtttggc ggcgtgaaag gtcgttccca
     5161 gttaccgggt atggttgaag atgcgatgaa aggtgatatc gatctggaac cgtttgtcac
     5221 gcataccatg agccttgatg aaattaatga cgccttcgac ctgatgcatg aaggcaaatc
     5281 cattcgaacc gtaattcgtt actgatttcc cgcaggtata ccccgtccac ttcagacggg
     5341 gttcttaata ctctccccgg gcagccgtcc gggggattaa ccgtgagata atgactgatg
     5401 gaactcattg aaaaacatgc cagctttggc ggctggcaaa atgtgtatcg gcattattcc
     5461 caatcactga aatgtgaaat gaatgtcggc gtctatctcc caccaaaagc cgcgaatgaa
     5521 aaattgccgg tgctgtactg gctttcaggc ctgacctgca acgagcagaa tttcattact
     5581 aaatcgggga tgcagcgtta cgcggctgag cacaacatta ttgttgttgc gccggacacc
     5641 agtccgcgag gcagtcatgt cgcagatgcc gaccgttacg atctcgggca aggtgccggg
     5701 ttttacctga acgcaacgca agcgccgtgg aatgaacatt acaaaatgta tgactatatc
     5761 cgcaacgagc tgccggattt agtgatgcaa cattttccgg caacgaccag aaagtctatc
     5821 tctggtcatt ctatgggcgg gctgggcgcg ctggtgctgg cgttacgtaa cccagatgaa
     5881 tatgtcagcg tctcggcgtt ttcgcccatt gtctccccat cgcaagtacc gtggggacag
     5941 caagcctttg ccgcatatct tggtgaaaat aaagatgcct ggttggatta cgatccggtg
     6001 agtcttattt cacaaggtca acgcgttgcg gaaatcatgg ttgatcaggg gttgagtgat
     6061 gatttttacg cggaacagct gcggactcca aatcttgaaa agatctgcca ggagatgaat
     6121 atcaagacgt taatccgtta tcacgagggt tacgaccaca gctattattt tgtctccagt
     6181 tttattggcg agcatattgc ctaccacgcc aataaactga atatgcgttg ataatagtgc
     6241 acgactgccg gatgcggcgt gaacgcctta tccggcctac acttcgcccg taaaccgtag
     6301 gcctgataag acgcgcaaag cgtcgcatca ggcatggcgc acgactgccg gatgcggcgt
     6361 gaacgcctta tccggtctac acttcgccca taaaccgtag gcctgataag acgcgcatag
     6421 cgtcgcatca ggcatctgcg cacgacttat ctatgtttac cacatcaaat catcaggcac
     6481 tttgaagtcg gcatacggat cgtcttcatc ctgctcttcg gcactgagcg cgctgtgcaa
     6541 cacaatactg ctggcatcac gctgagcaat tttatcggca acactggcgg gaataatggt
     6601 gtattcacct tcgctattgt tatcgaccaa caagcgggca atcgccagac gaccattaat
     6661 caactgcgcc tgtgtgagct tatcgacaaa aatcttctta atcagattgc cgtcagtgaa
     6721 gttaaaacca atatcgccat tggcaatggt gattcggttc atttcaatga gctgcttcac
     6781 ctgggcttta tattctttcg ccaacgccgc ttgtttttgc tgttcgctaa gctgtttatc
     6841 acgctcaagc tgtgcctttt tattttcttc taccgcagcc cgagcttcac gcgcctgaac
     6901 gcgcgatttt ttcgccgttc tctcgacctt cgccgctttt ttgctggtga ctaatcctgc
     6961 tttgagcaac tgctcttgta aggtaagttt tgccatcttt gtttccaaac ccgctgaata
     7021 atttttagcg agtatacctg taaacgccgt gggtgtattg ggggcaagtc cctttcgccc
     7081 ctgcttaata aggcgcgtca ggcatcggca cacggctgca ttcttgatct ccgactcatc
     7141 aaaataaaca ccgccaaccc agcaacaaga atacccggtg cagaagcggc cattacgccg
     7201 accgtgccag tgcctaatgc cagcattttc ccggccagta acggaccgct catagccccc
     7261 agacgcccta ccgccacggc tgttcccaca cctgttgcgc ggatctgcga actgtaaaac
     7321 aacagtgcca gggcatacaa aacgctttgc ccacctgtcg caaacaaccc cgcgacaaat
     7381 cccgccagca acataccgtt aaatgacgac accgttccaa gcgccagcag cgaagctaac
     7441 atgccgctat aaatcagtag cgacatggtt actggacgca gcttatccat caatgcgccc
     7501 aacattaacg tcccgcttgc cgcccccatt tgcagggcaa acatcacccc tgccgcctgc
     7561 gatggctgga atccttgctc caccaaaagt agcggtagcc agttgatcaa catataaacc
     7621 accagcagag tgaagaaata acacaaccac agcagcagcg tcgcggttgc cgtttctggc
     7681 gcaaataagg cacgcagtgg tggcgcagcc tgtttttcgc cagcgaaaac cgctgactcc
     7741 ggcagccagc gcattaatag cggcaccaga atcaacggca ccacaccacc tacccaaaac
     7801 accgtttgcc atgctaagtt tgcccccgcg aaacccagtg tcgccgccag cgccgcgcca
     7861 atgggaacac cgcaatacat caggctcact gccgtcccac gaaaacgtgg acccgcggct
     7921 tcagatgtca gggcgataag attcggcaac gccgccccca gcccgacacc ggtcatcagc
     7981 cgcgcaaaga ccagtgaggg gaaatcccag gcaatcgccg ttgccagtga gaacaaacca
     8041 aacagcgcaa ctgagccaat caaaatacgc ttacgaccat aacggtctgc cagcatcccg
     8101 ccaaccaacg cgccgggtag caaaccgagt attccggcgc taaatatcca gcccatttgc
     8161 attttatcga gtgcgaaagc ctgggcgatg ccacccgccg caatgccagc cgcctgaaga
     8221 tccagccctt ccatcagagc gaccaaaaaa caaagcccga tggtcagcat caggcgggat
     8281 gaagatgatg aaggggtacg agtcgacata agatgaacct cattgcgagt gcaggtgaat
     8341 cgcgactgat acattgccag ccgcgtaatc agaactattt aatgactgcc tacgggcgaa
     8401 atgtagcgaa atgtaggccg gataaggcgt tcacgccgca tccggcagtc gtgcgcagat
     8461 gcctgatgcg acgctaacgc gtcttatcag acctacaaat tctcacgcca tccgtaggcc
     8521 ggacaaggcg ttcacgccgc atccggctgt cgtgctggga tgggcataca aaatgcacgt
     8581 tatcggcagt catcattatt tgttgttgcg cagatccagc gccacgtcga cgatcatatc
     8641 ctcctggccg ccaaccatcc ggcgtttgcc cagctcaacg agaatatcca ccgcacttaa
     8701 gccataacgc gccgccgccg tttcacagtg acgcaggaag ctcgagtaaa caccagcgta
     8761 tcccagcgcc agcgtttcgc gatcgactcg taccggacga tcctgcaacg gacgcaccag
     8821 gtcgtcggcg gcatccatta acgcatagag atcggtccca tgctgccagc ccagtttatc
     8881 cgcggcggca ataaacactt ccagcggtgc gttacctgcg cccgcgccca ttcccgcgag
     8941 gctggcgtcg attcggtcgc agccctcttc caccgccgcg atagagttcg ccacgccaag
     9001 actcaggtta tggtgagcgt gcatgccggt ttgcgtttct ggtttcaaca ctgctttcag
     9061 ggcgcggaaa cggtcacgga tatcgctcat gttcatcgca ccgccagaat ccaccacata
     9121 aatacaggtc gcaccgtagc cttccatcag ctttgcctgc ttggcgagat tctccggcgt
     9181 ggtcatatgg ctcatcatca gaaaaccaac ggtgtccatt ccgagctcgc gggcatactg
     9241 aatatgctgg gcggaaacat cagcttcggt acagtgcgtt gccacacgaa ccacccgcgc
     9301 gccagcctgc caggcatttt tcagatcgtg aatagtgccg attcctggca gcaacaacgt
     9361 cgcgattttg gcgtgcctca ccacatccgc cgccgcttca atccattcaa ggtcgctatg
     9421 tgcgccgaaa ccatagttaa agctggaacc ttgcaaaccg tcgccgtggg ccacttcaat
     9481 cgaatccacg cgggcatcgt ccagtgcttt ggcaatctgg cgaacgtttt ccagcgaata
     9541 ctgatgacga atggcgtgca taccgtcacg caatgtgacg tccgagatat aaagtttttt
     9601 accgttcatg ccgcttctcc tgccttgcgc gccagtgact gggccatttt ttccgctgtc
     9661 gccagcgcac tggaagtcat aatatcgagg ttgcccgcat aggcaggcag ataatgcgct
     9721 gcgccttcga cttccagcca gaccgctgtt ttcagtccgg agaattgccc cacgcccggt
     9781 aaattgaccg gtttatcctg cgggataact tcaaactgca cgcgctgttt caggcgataa
     9841 cccggtacgt aagcctgcac cgcctcagcc atttcattga ttgaggcttc gatatcatct
     9901 tgtgaagctt cgtcgctcaa tacatacacc gtgtcacgca tcatcaacgg tggctctgct
     9961 gggttaagca caataatcgc cttccctttt gccgcgccgc ccaccacttc aatggctcgg
    10021 gaagtggttt ccgtaaattc atcgatattg gcacgcgtgc caggtccggc agatttactg
    10081 gcgatagaag cgataatttc ggcgtaatga acacgcgcca cgcgtgaaac tgccgccacc
    10141 attggaatgg tggcctggcc gccgcaggtg accatgttga cgttcagttg atcgacgttc
    10201 gcctcgaggt taaccaccgg cacgcagtaa gggccgatgg cagcaggcgt caggtcaatt
    10261 aagcgaatat ccggtttcgc ttcgcgtaaa gcggcatcgt ttttcacatg cgcgcccgcg
    10321 ctggtcgcat caaatacaat gtcgatatca gcaaattcag gcatgttcat cagcccgatc
    10381 accccttcat gggtggtggc gacgcccata cgtctggcac gcgccagacc gtcggactga
    10441 ggatcaatgc cgaccatcac cgccatctcc agatgctgac cgtgacgcaa aattttaatc
    10501 atcagatcgg tgccaatgtt gccagaaccg ataatggcga ctttacgctt actcatgaca
    10561 gacttccttt tggggctgcg cttgaaaatg tcgcagcaac tgaacctatg ccttcaatat
    10621 gggcttcaaa acgatcgccc gcattcaccg ccaccatcgg gcctaatgcc ccggtaagaa
    10681 tgatatctcc ggcgcgcagc ggttcaccca gactggccat tttgcgtgcc agccagacgg
    10741 cggcattaag cggatgtccc aggcattcgc tgccgcgccc gctggaaacc tcttcgttat
    10801 tacgcgtcat cttcatggcg cagtttttca ggtctaaccc cgccggacgt tgcgccggac
    10861 cgccgatgac atacaccccg caggaagcgt tatctgccac ggtatcgaca aactgaatcg
    10921 accagtcgcg aatgcggctc cccaccactt ccagcgccgg aagtacccat tcaatggcgt
    10981 tatacagttc gtcgaaggtg atatcagttg cgggtaaatc gcggttcaac accagtgcga
    11041 tctccgcttc aatgcggggt tgcagaacac gggaaaaagg aatgatttcg ttatcgccat
    11101 aacacatgtc ggcaaataac gtcccaaaat ccggttgatc aacgcccagt tgttgttgca
    11161 ctttcggatg cgtcaggccc actttacgcc ctaccacgcg acgcccctgc acaacgtcat
    11221 attgcacatt tatgtgctga atggcgtagg ccgcttcagc gtgatcgata ccaatcagat
    11281 cgcgcagcgg cgcaatcgct tcgccctgct ctgcggcgcg gcgtaaatcc gccgccagtt
    11341 gctcaagagt atgcttcgtc atgaccattc cttaagggcg tgcgaggaaa ttcagcacca
    11401 attgattgaa agcgtcggca tgttcccact gcgcccagtg accacagtcg cggaagatat
    11461 gcagttcaga accggcaatg ccggacagca gacgcagacc cgcatccatc ggcacaaagc
    11521 ggtcgttgcg cccccagaca atcagggttt gcgctttgat ttccgccaga cgtgggccaa
    11581 aatccgggaa ctgtttcgga ttagcttcca ggctcttaac gaagttttcc aggtgatcgc
    11641 ggcgcgacag catattattc aggcgcgctt caaacagggc gtcggtcaaa tcgctggtat
    11701 cgaaaacgaa gatatccatc atcagcttca ggttttcgat agtcggctga cgataaagct
    11761 gattcagtcg cttaatacct tcggttggca tcggcgtaaa caaactcatg ccgcccgtac
    11821 cgccgcccat cagcaccagt ttgccgacgc gctccggcca gttaagggtg aacgcca
//
LOCUS       DAJTZL010000127        11777 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.127, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000127 DAJTZL010000000
VERSION     DAJTZL010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11777
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.127"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..609)
                     /locus_tag="OO544_003002"
     CDS             complement(<1..609)
                     /locus_tag="OO544_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HCR6768534.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATAKRYPLTPLLYTLIFFHAIILMVGGQYTYAKVPIGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVIFLARFHCRQLRR"
     gene            complement(732..2378)
                     /gene="fumB"
                     /locus_tag="OO544_003003"
     CDS             complement(732..2378)
                     /gene="fumB"
                     /locus_tag="OO544_003003"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HCR6768535.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            complement(2456..3796)
                     /gene="dcuB"
                     /locus_tag="OO544_003004"
     CDS             complement(2456..3796)
                     /gene="dcuB"
                     /locus_tag="OO544_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HCR6768536.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            complement(4367..5086)
                     /gene="dcuR"
                     /locus_tag="OO544_003005"
     CDS             complement(4367..5086)
                     /gene="dcuR"
                     /locus_tag="OO544_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HCR6768537.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTDWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(5083..6714)
                     /gene="dcuS"
                     /locus_tag="OO544_003006"
     CDS             complement(5083..6714)
                     /gene="dcuS"
                     /locus_tag="OO544_003006"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     DcuS"
                     /protein_id="HCR6768538.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLANSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGKENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            6895..7125
                     /locus_tag="OO544_003007"
     CDS             6895..7125
                     /locus_tag="OO544_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HCR6768539.1"
                     /translation="MDQALLDEGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVATVVKVIDTCPSGALKYRHK"
     gene            7137..7409
                     /locus_tag="OO544_003008"
     CDS             7137..7409
                     /locus_tag="OO544_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR6768540.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
     gene            7636..7932
                     /gene="ghoS"
                     /locus_tag="OO544_003009"
     CDS             7636..7932
                     /gene="ghoS"
                     /locus_tag="OO544_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HCR6768541.1"
                     /translation="MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEY
                     GIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDIDFDLNIMTVDDYFRQFYK"
     gene            7960..8133
                     /gene="ghoT"
                     /locus_tag="OO544_003010"
     CDS             7960..8133
                     /gene="ghoT"
                     /locus_tag="OO544_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HCR6768542.1"
                     /translation="MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITW
                     PMSLPVALLFSLF"
     gene            complement(8252..9769)
                     /gene="lysS"
                     /locus_tag="OO544_003011"
     CDS             complement(8252..9769)
                     /gene="lysS"
                     /locus_tag="OO544_003011"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HCR6768543.1"
                     /translation="MSEQETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDH
                     TSDQLHEEFDAKNNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     SLPEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
                     SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDAAKALAESIGITVEKSWGLGRIITEIFDEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFQEQVNAKAAG
                     DDEAMFYDEDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPQK"
     gene            complement(10006..11463)
                     /gene="dtpC"
                     /locus_tag="OO544_003012"
     CDS             complement(10006..11463)
                     /gene="dtpC"
                     /locus_tag="OO544_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HCR6768544.1"
                     /translation="MKTPSQPRAIYYIVAIQIWEYFSFYGMRALLILYLTHQLGFDDN
                     HAISLFSAYASLVYVTPILGGWLADRLLGNRTAVIAGALLMTLGHVVLGIDTNSTFSL
                     YLALAIIICGYGLFKSNISCLLGELYDENDHRRDGGFSLLYAAGNIGSIAAPIACGLA
                     AQWYGWHVGFALAGGGMFIGLLIFLSGHRHFQSTRSMDKKALTSVKFALPVWSWLVVM
                     LCLAPVFFTLLLENDWSGYLLAIVCLIAAQIIARMMIKFPEHRRALWQIVLLMFVGTL
                     FWVLAQQGGSTISLFIDRFVNRQAFNIEVPTALFQSVNAIAVMLAGVVLAWLASPESR
                     GNSTLRVWLKFAFGLLLMACGFMLLAFDARHAAADGQASMGVMISGLALMGFAELFID
                     PVAIAQITRLKMSGVLTGIYMLATGAVANWLAGVVAQQTTESQISGMVIAAYQRFFSQ
                     MGEWTLACVAIIVVLAFATRFLFSTPTNMIQESND"
     gene            complement(11522..>11777)
                     /locus_tag="OO544_003013"
     CDS             complement(11522..>11777)
                     /locus_tag="OO544_003013"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="constitutive; catalyzes the formation of cadaverine
                     from lysine; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HCR6768545.1"
                     /translation="TEEVYLDEMVGRINANMILPYPPGVPLVMPGEMITEESRPVLEF
                     LQMLCEIGTHYPGFETDIHGAYRQADGRYTVKVLKEESKK"
BASE COUNT         3042 a         2856 c         2892 g         2987 t
ORIGIN      
        1 gcgccgtagc tggcggcaat gaaagcgagc gaggaatatc accgtcgtta atgcgccaag
       61 cagcgcgcag aacatatcgg attgcgtgtc ccactggtcg ccctgggtgc cgagaaaatc
      121 atccgctccc tggcccattg ccagcgccgc ccaccactcg attaattcat acatcgcgct
      181 tatcgccagc gccacgcagc agactaggaa cgccaccatt ttacgtccgc gcacgtacat
      241 cccgcgcacg agaatttctc gtgccaccag tgcaggcacc agcccctgga aaaaatgccc
      301 cagcttgtca tacggattac ggctcaaccc cagccattcc tgcacctcaa aaccgatggg
      361 gactttcgcg taggtgtatt gcccgccgac catcaggatg atggcgtgaa agaaaatgag
      421 cgtatagagc agcggcgtta acggataacg tttagcggtg gcaagcagta gcggcacgac
      481 aataatcacc ggtgtcactt ccatcagcca ggtgagtttg tcatgggccg aaatgccggt
      541 ataaatcagg attaacgtta gcgccagtgc gccggtgtta agaattaacg gtttgagtgt
      601 gcgggtcatg gtgagttcgc aagtcaggga aaatactgac tattcaccag caagcatgaa
      661 attacaatcg caaaagaggt ggcccagggg gatcacctgg cagcatgctg ccaggcgctg
      721 ggccgaagag gttacttagt gcagttcgcg cactgtttgt tgacgatttg ctggaagaag
      781 tcgttacctt tgtcatcgac caggataaac gccgggaaat cttctacttc gattttccag
      841 atagcttcca tacccagctc cggataagcg acgcattcca gatgcttgat gctctgctgc
      901 gccagtaccg ccgccggacc gccgatgcta ccgaggtaga agccgccgtg tttatgacac
      961 gcgtcggtaa cctgctggct gcggttgcct ttcgccagca tgatcatgct gccgccgtgg
     1021 gattgcagca gatccacata ggagtccata cgacctgcgg tggttgggcc aagtgaacct
     1081 gatggataac cggcaggagt tttcgccgga cccgcgtagt agatcgggtg atctttgatg
     1141 tactgcggaa gttctttacc ggcgtcaatc agctctttca gcttggcgtg tgcaatatct
     1201 cgacccacaa taatggtgcc agtgagcgac aaacgagtgg aaaccggata ttgcgaaagc
     1261 tgggcgagga tctctttcat cgggcggtta aggtcgacct tcaccgcttc gccttcaccg
     1321 gcctgacgca gttcttgtgg aatgtactga cctggattgt gttccagttt ttcgatccag
     1381 ataccttcgc ggttgatttt cgctttaatg ttacggtcag cggagcagga gacgcccatg
     1441 cctaccgggc aggatgcgcc gtgacgtggc agacggataa cgcgaatgtc gtgcgcgaag
     1501 tatttaccgc caaactgcgc gccaagaccg agtttctggg cctcttccag cagttcctgt
     1561 tccagctgga catcgcggaa cgcctgacca tgttcgttcc cttccgtcgg cagttcatcg
     1621 taatagtgag cgcttgctaa tttgacggtt ttcaggttgg tttccgcaga cgtaccgcca
     1681 atcacaaacg cgatatggta cggcgggcag gctgcagtac cgagggtacg cattttctcg
     1741 acgaggaagt ttttcagttt gccgggagtc agcagggctt tggtttcctg gtagagatac
     1801 gttttgttgg cagagccgcc gcctttcgca acgcaaagga atttgtactc atcgccatct
     1861 accgcgtaca ggtcgatttg cgcaggcagg ttagtgccgg tgttgacctc tttgtacatg
     1921 tccagcgccg cgttttgcga gtagcgcagg ttatcttcga tataggtgtt atagacgcct
     1981 ttcgacagcg cttcttcatc accgccaccg gtccacacgc gctggccttt tttaccgacg
     2041 atgatcgcgg tgccggtatc ctggcaggtc ggcagcacgc ctttggcggc gatttcggag
     2101 tttcttaaga attgcagtgc cacgtacttg tcgttttcgc tggcttctgg atcgtgaaga
     2161 atagccgcaa cctgtttctg gtgtgccgga cggagcatga aagaagcgtc gtgaaaggct
     2221 tgctgcgcca gcagggtcag ggcttctggt tccactttca ggatggtttc gccgtcgaag
     2281 tcggcaacgc taacgtaatc ggaagtgagt agatagtatt cggtattgtc tttccccatc
     2341 gggaaaggtg cctggtagat aaagggtttg tttgacatag cttccagcct gtaactctgt
     2401 atttgttatt cgaaaatggc gtgccgcgta gtcacgggcg gcacgcaaag tgcatttata
     2461 agaacccgta catcgcggcg aagatccagc cgaagacgca cgatacgctc acaccaatca
     2521 accccggcag aataaagctg tggttgatga cgaagcgacc gatgtgggtg gtgccggaac
     2581 ggtcaaactg aatcgctgcc agatcgctcg gataagtcgg caggatgtaa taaccgtagc
     2641 aagccggtgc tgaagccacg atgtatgccg gatcaacgcc gatcgccagc gcgaccggaa
     2701 caatcgccgc cagcgccgca gcctgagagt ttacaaactt ggaaaccagc agcagaacaa
     2761 tggcataggc ccacggatac tctttcacca tttcacccag tacgccctga atttcagaca
     2821 tatgcgcacc gaacatggtt tctgccatcc atgcgatacc gtacaccgcc acgatggcga
     2881 tcataccgga acggaagact tcgttttttg agatagacgc gggattggtt ttggtcagga
     2941 taataatcag cgccccggtc agcagcataa acatctgaat aaccagtacc atcgacagcg
     3001 gtttgccgcc gaaggatgga cgcaggtccg aatcagcacc aagaagggcg actacagcga
     3061 ttgccccgag gaaaatccac attgccagcc agttgctttt cggcagtttt ttgtccagca
     3121 gcgtcgcggt atcaccgtaa acatactcac ggttttccgg tacggagatg aatttctgga
     3181 actcttcgtc tttatccaga tctttaccac ggaaccagct gaagataccg atcgccagga
     3241 taccgattaa cgtcgatgga atggtgattg ccagcaggtc gaggaactca agatggcgac
     3301 catcaaaggt gacattaccc agcattgcaa ccagagacac gaccgcaacc gacaccggac
     3361 tggcgatgat ccccatctgt gcaccgatag aacttgccgc catcggacgt tccggacgga
     3421 tgttgttctt aatggcgacg tcgtagatga tcggcagaat ggtgtaaacc acatgacccg
     3481 taccgcaaag aatggtcagc gtacaggtca caaacggcgc gacaattgag acatatttcg
     3541 ggttgcggcg cagcagcttc tcggcaattt gcagcatgac atcaagaccg cccgaagctt
     3601 gcaaggtcgc cgatgccgcc accaccgcaa tgataaccag catgacatca accggtggtt
     3661 tacctggctg aaggtggaag acgaagacca gaatgaccag accgataccg cctaataaac
     3721 ccagcgcgat accacccttt ctggcaccat aaaacagaca tatcagtatt atgataagtt
     3781 ggatagtaaa taacatgtgt gaaccctcgc gataatccta tttaaatttt tgctgaatag
     3841 atcacagtca cgttctgttt tgtatgaact gttttcagag cctgactaaa tatccgtttg
     3901 gtcgttaccg gctttagcaa atacctcaca gtgaatattg gctgattaaa gcagcggcaa
     3961 atttatgcac tgtgataagc ggctttttca gagagaaaga ctctctggcg atgggtttat
     4021 agtttgtact gcgcagatgt taatgttttg tttgctgtta tcttattgat atttatcggt
     4081 ctattttttg ttctcagggc agatgtcatt aggtttatag attaatctga tctacccatt
     4141 tgtgggtaaa aatacacata atgcgggtga cataatagtt aattaacttt tgttagcgtt
     4201 ttgaaattaa aaacaccgtt cacctgaaga gatattaatt tttagcgatg atgcagggat
     4261 aattatattt gatctggcac aagttttact gatgaaggat gtaacttgtg ccaggggtat
     4321 tttgcattac ggtaataatt attacttaca gataaccagc gatccgttat tggcaatatt
     4381 gtttcagtag tgagtagtgt tctgcctgaa tacggtaacg gtaaactgga cgccccgtga
     4441 cgccataatg gatactggtg aacaagatgt ggcagttgac cagccagatg aggtatttac
     4501 ggcaggaaac acgcgaaatg ttaacctcgt tggctagctc gtcggttgaa aattcatagt
     4561 cctgatgcgc gtcaatccac tggcacagtg tgcgtaacgt ctgcggcgtt aagccttttg
     4621 gcaagcgacg aggatcctgt tcgttggagc tgctgccgtg gattagctga tcaagctcgg
     4681 cctggtcata atactgatgt ttttccagcg ccattttctt ttgccgccag tcggtgagcg
     4741 cctcttcaaa gcgggaagcc tggaagggtt tgatcaggta atccacgaca ccgtaatgca
     4801 gcgaatcttt aatggttgcc gcatcggctg cggaggagat gacaatcaca tcacttttgc
     4861 aacgcgcgtt atgcaggaca ggcagtaaat cgagcccgtt ctctttttgc atatagatat
     4921 cgagcaatat caggtcgata ggcgtatcgc tattgaagat aatctctttg gctttctcca
     4981 gcgtcgaggc tgttccacag cattgaaagc ctgggatttg tgctacgtat cggcgattca
     5041 gctccgcgac cattgcgtcg tcatcgataa ttaatacatt gatcatctgt tcgacctctc
     5101 cccgtcccag ggtatctgga caaaaaattg tgtgaaaatc ccgggttccg attccacggc
     5161 gatgctgccg ccgagatttt ctacctgttg tttgacaagt gctaaaccga cgcctcgctc
     5221 gcttcctttt gtcgagacac ctttgtcaaa aatgtgatcg attttgtcgg gtgcgatccc
     5281 cggtccatca tcgttaactt cgcagtgcag ccagccgtga cggtagtgca atgttacgct
     5341 aatttcgcca ccgggttccg gccctaatgc ttccagcgcg ttttctatca gatttcccaa
     5401 tgtggtaatc agcgtcgcga cctggtcctc actgccgctg tctggcagct ggctttcact
     5461 gtttaaaatc agcgtatggc ctaaatcggt cgcgcgatta atcttgctga ttaaaaaacc
     5521 agcgataacc ggagatttga tcttacccag cagagagcca atctcttcct gatagttatt
     5581 ggctgttttt agaatgtaat cttccaactg cttataactc ttcagatgca ataatccgag
     5641 aatcacgtgc aatttattca taaattcgtg ggatcgttca cgaagtgcgt cggcatagtt
     5701 gaccagaccg tcgagtcgct gcatcagttt acgtacttcg gttttgtccc tgaaggttga
     5761 aatggcaccg atgataacgc cattactgcg caccggaacg gtgttgatca gtaatagccg
     5821 gtctttaatc gtaatctctt cgtcgcggcg cggcgtaccg tcgcgtaaca cttccgagac
     5881 atctaccacc tgtgaccatg agtggctgag cgtcgacagt ttctcatcgt cctgcgactt
     5941 acggtaattc agcaattctt gtgcggcatc gttgatcagc gtgacctcgc cgcgatcgtc
     6001 cacggcaacg acgccttctt tgatagactg caacatggcc tggcgttgct caaacagcgt
     6061 ggagatttcg tagggttcca ggccgaaaag gatttttttc agtaccttaa ccagaatgca
     6121 ggtgccaatc agtccgacca gcatgccaaa taataccgac cagataatgc tccagcgact
     6181 gtcattgatt tgttgggtca cgcggcttaa ctcaaggccg atcgccacca cgccaatttg
     6241 tttatgattt tcatcgtaga tgggggtaaa tacgcgtaaa gcctgcgcca gaaaaccgcg
     6301 attgatagcg acattttctt tgccattcag cgctttaagg atgtcatcac ctttaaatgg
     6361 ctgaccaata cgctgggctt caggatgcga gtagcgaaga ctttgcatat cggtaacgac
     6421 aataaacagc agatcgttgc gtttgcgcac ggcttccgcg atggcctgga tgccgctctc
     6481 ctgcggtttt ttctgcaagc cctgacggat ttccggcgag ttggcgaggg tacgcgccac
     6541 tgccagtgcc ttgttggcta gcccgtctcg cgtcatatca ctgatttgcg agaagtaaat
     6601 cagatgcacc accaatagca ccgagaacag taccgcactg accattaaga tcactgtggt
     6661 actcaatttc atcggacgtt tgcgtaacat gcggtagggc aatgaatgtc tcatcagctt
     6721 ccttgtgtga caaatttctt aaacattatc tctgatgagg tgggtaattc aaagggagta
     6781 agaatgattg gctatatagg ggaagagatt ctggcaacgg aaactgccag tgctgtatga
     6841 agattccagg gctatgctta tagcgataat catactgatg agagagggaa agtcatggat
     6901 caggcgctac tggacgaggg ttatcgctgt tataccggcg aaaagatcga tgtctatttc
     6961 aacactgcga tatgtcagca ttctggcaat tgcgtacgtg gcaacggcaa gttatttaat
     7021 ctcaaacgaa agccgtggat catgccggat gaagtcgacg tcgccactgt ggttaaagtg
     7081 attgatacgt gcccgagcgg cgcgctgaaa taccgtcata aataagcgag ggtaaaatgg
     7141 aaatacgcga aggccacaat aaattttaca ttaatgacga acaaggcaag caaatcgctg
     7201 aaattgtctt tgtgccgact ggagagaatt tagcgattat cgaacatacc gatgtcgatg
     7261 aaagcctgaa agggcagggg attggtaaac agctggttgc aaaagtcgtg gaaaaaatgc
     7321 gtcgggaaaa acgaaaaatt atcccactat gcccatttgc gaaacacgaa tttgataaaa
     7381 cgcgggagta tgatgatatt cgcagttgat gggagagtac agagtcacga tatttttcat
     7441 gctctccgcg gagtgatgca ggagagcatc tgaagggtag ggggatgcac aaagaatggg
     7501 cagagagcgc gtttttttgt cccaagtcat cccctttact gagcaaaaaa aagaatatct
     7561 cctatatgag aatcatcaat cggggttatc aagttttgcg tccccagagc attcaatatt
     7621 gataggagtc atattatgga aggtaaaaac aagttcaata cttatgttgt ttcttttgat
     7681 tatccatcat cttattcctc agtgttctta aggttaagat cattgatgta tgatatgaat
     7741 ttctcttcta tcgtggctga tgaatatggg ataccacgac aattgaatga aaattccttc
     7801 gcaataacga catcgttagc cgcaagtgaa atcgaagatt taatcaggct taaatgctta
     7861 gacttaccgg atattgattt tgacctcaac attatgacag ttgatgacta tttccgtcag
     7921 ttttacaagt agccaggaag gataagaaga acataaaaaa tggcactatt ctctaaaata
     7981 ttaatttttt atgtgattgg tgtgaacata tcctttgtca ttatctggtt tatctcacat
     8041 gagaaaacac atattcgttt acttagtgca ttcctggtcg gaataacctg gccaatgagt
     8101 ctgcctgtgg cattactttt ttctctcttt taggagctgt agttgagtgg acccgctggt
     8161 tatcgtgaaa cgaagcgtct ctcctcaaaa ctggatttgc ggaacgacga acgaagcagt
     8221 cgcctgactg cttcgttcat taaagtgaaa tttatttctg tgggcgcatc gccgggaaga
     8281 gaataacgtc gcgaatagta tgactgtttg taaacagcat aatcattcgg tcgataccaa
     8341 tacccagacc agcggttggc ggcagaccat attccagcgc agttacgtaa tcttcgtcat
     8401 agaacatggc ttcgtcgtca cctgcagctt tagcgttaac ctgttcctgg aaacgttcag
     8461 cctgatcttc tgcgtcgttt aattcgctaa aaccattacc gatttcacga ccaccgatga
     8521 agaattcaaa acggtcggtg atttccgggt taacatcatt acggcgcgcc agcggtgaca
     8581 cttctgccgg atattccgta ataaaggttg gctgaatcag atgtgcttct gccacttcat
     8641 caaagatctc cgtgataata cgtcccagcc cccagctttt ctctaccgta attccgatag
     8701 attcagctaa tgctttagca gcatcaaaat tatccaggtc ggccatatcg gtttctggac
     8761 gatatttttt gattgcttcg cgcatggtga gtttttcaaa cggtttgccg aaatcaaaca
     8821 catgctcgcc ataagtgact ttagtggtac ccagaacctc ttgtgccagg gtgcggaaca
     8881 gtgactctgt cagttcaatc aaatcgtggt aatccgcata cgccatgtag agttccatca
     8941 ttgtgaactc aggattatgg cgaacagaaa taccttcatt acggaagtta cggttgattt
     9001 cgaatacccg ttcaaaaccg cctacaacca gacgtttcag atacagctcc ggcgcgatac
     9061 gcagatacat atctaaatcc agagcattat gatgggtaat aaacgggcga gcagatgccc
     9121 cacctggaat tacctgcatc atcggggttt ctacttccat aaagccgcgc gcgaccatga
     9181 actgacggat agcggccaga atttttgaac ggacaacaaa cgtttgacgg gatttatcgt
     9241 tagcgatgag gtccagataa cgttgacgat aacggacttc ctgatcctgc agaccatgga
     9301 atttatctgg taaaggacgt agtgctttag tcagcaggcg caactcagta cagtgaatgg
     9361 aaagctcacc cgtttgcgtc ttaaacagcg taccgcgggc accgataatg tcacccagat
     9421 cccatttttt aaactgatcg ttataaacac cttctggcag gctatctctt gcaacgtaca
     9481 gttgaatacg gccaccgaca tcctgcaacg ttacaaagga ggctttcccc atgatacgac
     9541 gggtcatcat tcggccagca accgagactt caatgtttaa ggattccagt tcctggttat
     9601 tcttcgcatc aaactcttcg tgcaactggt cagaggtatg gtcgcggcga aaatcattgg
     9661 gaaacgccac accttgctga cgtagtgccg ccagtttttc gcggcgattt ctcagttcat
     9721 cgttaaaatc aatagcctca ttggctcccc gtgtttcttg ttcagacatt ttggttcctc
     9781 taaatccagc tttcaattta gcgtagataa agagacagat cggtacactg tattatctgc
     9841 ctcgactatt aataactcag tttatcttat cgaaactaac gcttacctca agttgatatc
     9901 actaataaat aaccatcaaa atggtttaaa tgacttatta atctcgattt actagtgtga
     9961 ttaactgaaa gttaatttat tcataaccaa ttgtttttac gacagttaat cgttgctctc
    10021 ctgtatcata ttcgtcggcg tgctaaacag aaaacgggta gcaaaggcca atacgacgat
    10081 gatcgcgaca caagccaacg tccactctcc catctgagaa aagaatcgct ggtaagctgc
    10141 aattaccata ccgctaattt gcgactctgt cgtctgctgt gccacgacgc ctgccagcca
    10201 gttggcgacc gcgcctgttg ccagcatata aataccggtt aatacgccag acattttcag
    10261 acgcgtgatt tgcgcaatcg ccaccgggtc aataaagagt tctgcaaagc ccattagcgc
    10321 caacccggat atcatcacgc ccattgacgc ttgaccgtca gccgctgcat gtcgggcatc
    10381 aaatgccaac aacataaagc cacaagccat cagcagtaag ccaaaggcaa acttcagcca
    10441 gacgcgcaat gttgagttgc cgcggctttc tggcgacgcc agccaggcca gtacaacccc
    10501 agcgagcatc actgcaattg cattcaccga ctggaatagt gctgtaggta cttcaatatt
    10561 gaatgcctga cgattcacaa agcgatcgat aaacaagctg atggtactgc cgccctgttg
    10621 tgccagtacc cagaacaacg tcccgacaaa catcaacaat acaatttgcc aaagagcacg
    10681 gcggtgttcg gggaatttga tcatcatgcg ggcaatgatt tgtgcggcaa tgaggcaaac
    10741 gatcgccagc aaatatccgg accagtcgtt ctccagcagc agggtaaaaa atactggggc
    10801 taaacagagc atcaccacta accagctcca tactggtaag gcaaatttga cgctagtgag
    10861 cgctttttta tccatactac gcgtggactg gaaatgacga tgaccgctta agaaaatcaa
    10921 caaaccgata aacatgccgc cacccgcaag ggcaaagcca acatgccatc cataccactg
    10981 agcagccagg ccgcaggcga ttggggcggc gatagaaccg atattgcccg cagcatacag
    11041 cagcgaaaaa ccgccatcac gtcgatgatc gttctcgtcg tagagctcgc caagcaaaca
    11101 gctgatgttt gatttgaata aaccgtagcc acaaataatg attgccagcg ccagatacag
    11161 gctaaaggtt gaatttgtat caatgcccag caccacatgg ccaagggtca ttaacagcgc
    11221 gccggcaatc actgcagtgc ggttgccgag caggcggtcg gcaagccagc cgccgagaat
    11281 aggggtaacg taaaccagag aagcatatgc gctgaacagg ctgatggcat ggttatcatc
    11341 aaaaccaagc tgatgggtga gatagagaat gagtaaggca cgcatgccgt aaaaactgaa
    11401 gtactcccag atttggatcg ccacgatata gtatatcgcg cgcggctgtg agggtgtttt
    11461 catgtgttct ccttatgagc aaaaaaggga agtggcaagc cacttccctt gtacgagcta
    11521 attatttttt gctttcttct ttcaatacct taacggtata gcggccatca gcctgacggt
    11581 atgcaccgtg aatatcggtt tcaaatcccg gatagtgagt gccgatttca cacagcatct
    11641 gcaggaactc cagaaccgga cggctttctt cggtgatcat ttcacccggc attaccagag
    11701 gaactcccgg cggatacgga aggatcatat tggcgttaat acgacctacc atttcgtcga
    11761 ggtaaacttc ttcggtc
//
LOCUS       DAJTZL010000128        11541 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.128, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000128 DAJTZL010000000
VERSION     DAJTZL010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11541
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.128"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            459..962
                     /locus_tag="OO544_003014"
     CDS             459..962
                     /locus_tag="OO544_003014"
                     /inference="COORDINATES: protein motif:HMM:NF026057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6768546.1"
                     /translation="MENWVIQELKSLDIGDSRLDKRVKHILNSLSRSPEESIPVSCRT
                     WSETKAAYRCFSNDKVSANKIMAPHKENITERAQQFKRVLVLQDTTELNYSGQKEKQG
                     VGHKKHKDERNLFLHPQLVISESGVCLGVYDDYQWFRDELKTSKNTRQEICNDLLHKK
                     HVSEKET"
     gene            complement(1335..2528)
                     /locus_tag="OO544_003015"
     CDS             complement(1335..2528)
                     /locus_tag="OO544_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6768547.1"
                     /translation="MSPGIEDTPQKTLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFVTDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
     gene            2664..4388
                     /gene="iucA"
                     /locus_tag="OO544_003016"
     CDS             2664..4388
                     /gene="iucA"
                     /locus_tag="OO544_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HCR6768548.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            4389..5336
                     /gene="iucB"
                     /locus_tag="OO544_003017"
     CDS             4389..5336
                     /gene="iucB"
                     /locus_tag="OO544_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase"
                     /protein_id="HCR6768549.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            5336..7078
                     /gene="iucC"
                     /locus_tag="OO544_003018"
     CDS             5336..7078
                     /gene="iucC"
                     /locus_tag="OO544_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucC"
                     /protein_id="HCR6768550.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFVEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDATSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMNKHPQMAERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            7075..8412
                     /gene="iucD"
                     /locus_tag="OO544_003019"
     CDS             7075..8412
                     /gene="iucD"
                     /locus_tag="OO544_003019"
                     /EC_number="1.14.13.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase"
                     /protein_id="HCR6768551.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFARNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWRLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSRKKPQPEAASLTHYTANIQE"
     gene            8418..10613
                     /gene="iutA"
                     /locus_tag="OO544_003020"
     CDS             8418..10613
                     /gene="iutA"
                     /locus_tag="OO544_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HCR6768552.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLGQELVGQVYYRDESLRYYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IAAGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLSLTKSVNVSDSKLEGVKVDSYELGWRFTGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYINWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWQLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYCPGYGPASLYDYKGRGRTFGLNYSVLF"
     gene            complement(11164..11307)
                     /locus_tag="OO544_003021"
     CDS             complement(11164..11307)
                     /locus_tag="OO544_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6768553.1"
                     /translation="MEHRSVIHGELAIDHDAILQCAEEVLHHCVIQTVSFVWHILCYS
                     MGL"
BASE COUNT         2927 a         2895 c         2949 g         2770 t
ORIGIN      
        1 tcaacgaagg ggccgacaaa cacgttgtcc ctgagttcac attcataaat gttgaccggt
       61 ttgacaatac ggacattgat cccataaatc acatcccgga cagcacattc cagaaatttc
      121 atactcacac gtcctttaaa tacagatgac atttgccgga attataccgc ccagattcac
      181 ttgccatgtt tcagaatagc ttataagccg cacttttacc gatactcagc aacaaatcag
      241 gtctgtatcc acttcagccg gaaaccggat ctgcgcggcg gggaacgggc aagaaatatg
      301 ataattaagc aacacacata aaatcatatt gcataatcgc atctgaatcg aacacttatt
      361 cgcatcacta tcgatcatca cttcgcatct cataccctta cccacaagtt gaaataatag
      421 tattatattg aaaagacaga gattatttca gaggtgttat ggaaaactgg gttattcagg
      481 agctaaaatc actggatata ggggactcaa gacttgataa aagagtaaaa catatcctca
      541 atagcctcag tcgttcacca gaagaaagta ttcctgtttc ctgccgtacc tggagtgaaa
      601 caaaggcagc ttatcgttgt ttttcaaacg ataaagtctc tgctaataaa attatggctc
      661 ctcacaaaga aaatatcact gaaagagctc agcagtttaa aagagtttta gttttacagg
      721 atacaacaga acttaattat tctggtcaga aagaaaaaca aggagttggt cacaaaaaac
      781 ataaagatga aagaaactta tttcttcatc ctcaactcgt catcagtgag tcaggtgttt
      841 gcctcggtgt ttatgatgac tatcagtggt tcagggatga attaaaaacc agtaaaaata
      901 cccgacaaga aatctgcaat gatttacttc ataaaaaaca tgtcagtgaa aaagaaacct
      961 agcgttgggt tgaaggatat aataaagcaa cagaactggc tcgccaatgc cctgataccc
     1021 atgtattgtc aatttctgat cgtgaaggcg atttttatga cctgtttgaa cgcgcagagc
     1081 agactcccgg aataaaagct gactggcttg tcagaatgaa atttaataac agagcaacgc
     1141 tgaatattaa tggtaaacgg gatcataggt tgttgcacga aagaatcatg gagattacac
     1201 cgcagcaact ggttgaattc actatacctg atgggcgggg gcagcaagcc agaacactgc
     1261 ctgacatgct ttcatcagac gcaggtcctc tctcatatcc tctgatttac tccagtaagg
     1321 tgatgatcat atctttattt gatagtacgg taatgccaga taaatacaga cacaatggta
     1381 aataatatag ttgcaaataa tgctgccata atcagcgcga ttgcgacacc tgttttcgat
     1441 accagcggca ccagcaaagc gcacagtcca tagccaagtg tgtgactggt agcgagccag
     1501 cccgcgcctg cgccatcagc cagcttatca ttcagtagaa gttgataagc gggtgttgcc
     1561 agagctgctc caaatgacag cactgcacag cccgggtaaa acaaccatag ctgttcggag
     1621 agcatgatag ccagaccacc actcatcaac actccggcac tcaggagcag ggccaccgga
     1681 gtcaggcgct gcggacggag taccccgaac tgcgcgataa gcgcagctac tgcggacaga
     1741 cccaacagcc aggccacctg ttggctaatg gcggtggtat ccgtgacgaa ctggcgagta
     1801 agggcaggcg aaagtccaag ctgcatcatg ctcaccgccg cagccagtaa tattgcgcaa
     1861 agcagataag gcagacaatc ccgcttcaga ctgacgctct tgcactccgg tgtgggctgt
     1921 ggtggtgtgc cgggcaaccg cagcagcatc agcagcgcca gcactggtgc tgccatcagt
     1981 agccccagtg gcgccagagg atgaatggcc agcattgctg ccgcgcacag cggcccgaat
     2041 agcctgccgc aactcaggcc ggagctgatg gttgccagag cggccatgcg attcccttct
     2101 ccagctctct gcaatgccca gacctgacag gctggcacca ttgccgacac ggtcagcccg
     2161 taggcgatcc gggcgatgat taatattccc agtcccaccg ctgttgttat ggcgctggta
     2221 gccatcagca cgcttcccag ccctagcagg gtaaagctta tgccatatcc tcccagagcc
     2281 cacaacacca caggtttact gccagtacgg gaaatttgct ttccccacca cggggacgat
     2341 ggcagaaaca gcattgagcc aagcatcagc agggcagccc atacagacag actcagattt
     2401 gtctgtatga ccagaacagg gagcacaacc agcagaccgt tctgtccgat accgagaagc
     2461 ccggcactga acgcaagtgg ccagcaggac agtgtttttt ggggcgtatc ttcgatccct
     2521 ggtgacatta taatgtttca actccatgta ttaattgtgt ttatttgtaa aattaattta
     2581 tctgacaata acatttattg ttgataatga gaatcattat cgacataatt gttattattt
     2641 tactgtgtgg gagctgtttg actatgaccc tgccctctga aaaaccagcc acagatgtgg
     2701 ctgcgcagtg cttcctgaat gcactgattc gtgaaaccac agactggaaa ctgacagaat
     2761 acccgccaga cgaattgctc atcccgctgg atgagcagaa atcgctccat ttcagagtgg
     2821 cttatttctc cccaacccag catcaccgct ttgcatttcc tgcacgtctg gtcacggcat
     2881 caggcagtta tcctgtcgac tttaccactc tctcccggct tattattgat aagctacgcc
     2941 atcaactgtt tctgcccgtt cccctctgcg aaactttcca ccagcgcgtg ctggaaagcc
     3001 atgtccatac gcaacaggca attgatgccc gtcatgactg ggccgctctg cgtgaaaaag
     3061 cgttgaattt tggcgaggct gagcaggcgc tgctgacagg acacgctttc caccctgcgc
     3121 ctaagtctca tgaaccgttt aaccgacggg aggctgaacg ctacctgcct gatatggcac
     3181 cccacttccc actgcggtgg ttttcggtgg ataaaacgca aatcgccggt gaaagtttgc
     3241 atctcaacct tcaacagcgg ctgacgcgat ttgccgcaga gaatgcgcct cagttactca
     3301 acgaattaag tgacaaccaa tggctgttcc cgttgcaccc gtggcagggg gaatatcttt
     3361 tgcagcaggg gtggtgccag gcacttgttg ctaaagggct gattaaagac ttaggtgagg
     3421 ccggcacgtc gtggctgccg accacctctt cccgttccct ctactgtgcc accagccgcg
     3481 atatgatcaa gttctccctg agcgtacggc tgaccaactc catccgtact ctgtccgtga
     3541 aagaagtgaa gcgtgggatg cgcctggcac gcctggctca aaccgacggc tggcagatgc
     3601 tacaggtccg cttcccgact ttccgggtaa tgcaggagga tggctgggcc gggctgctcg
     3661 atcttaacgg caacatcatg caggaaagtc tgtttgccct gcgtgaaaat ctgctggtgg
     3721 atcagccgaa aagccagact aacgtactgg tctccctgac tcaggccgca cctgatggcg
     3781 gtgattcgct gctggtttcg gcggtaaaac gcctgagcga tcgcctcggt atcactgtgc
     3841 aacaggccgc ccatgcatgg gtcgatgcat actgtcagca agtgctaaag ccgctgttta
     3901 cggctgaagc ggattacggc ctggtgctgc tggcgcatca gcaaaatatt cttgtccaga
     3961 tgcttgggga tctgccggtc ggatttattt accgtgactg tcagggcagc gcttttatgc
     4021 ctcacgcgac agactggctc gattctattg gcgaggcgca ggcggaaaat attttcaccc
     4081 atgagcagtt gctgcgctat ttcccttatt acctgctggt taactccact tttgctgtga
     4141 ccgctgcgct gggggctgcc gggctggaca gcgaatcgaa tctgatggct cgtgtacgag
     4201 catcgctggc tgaagtgcgt gatcaggtga ctcataaaac ctgtctcaac tacgtgctgg
     4261 aaagtccgta ctggaacgta aaaggtaact ttttctgtta tctgaacgat cataacgaga
     4321 acaccattgt tgacccttcg gtgatctatt tcgatttcgc taacccgctg caggctcagg
     4381 aggtctgaat gtctgaggca aacattattc acagcagata tggactgcgc tgtgaaaaac
     4441 tcgacaagcc cctgaatctt ggctggggac tggacaatag cgcggtgttg cactgtcccg
     4501 gggagctgcc gacagggtgg ctgtgcgatg cgctggatca gatatttatc gccgcaccac
     4561 aactgtcagc agtggctctc ccctgggccg aatggcgtga ggagccacag gcgctgacgc
     4621 tttttgggca ggtaaaaagc gacattatcc atcgtactgc cttctggcag ttaccgttat
     4681 ggttgagttc accggcaaac cgggcctccg gtgaaatggt ttttgatgca gagcgtgaga
     4741 tttatttccc gcagcgccca ccccgtccac agggtgaggt ttaccgccgt tacgatccac
     4801 gtattcgcag gatgctgagt ttccgcattg ccgatcccgt ttctgatgca gaacgtttca
     4861 cccgctggat gaatgatccg cgcgttgagt atttctggga gcaaagtggc tcactggagg
     4921 tacagaccgc ctatctggag cgccagctca ccggtaaaca tgcattcccg ctgattgggt
     4981 gcttcgacga tcggccattc agctatttcg aaatctactg ggcggcggaa gaccgcattg
     5041 gtcgtcacta ttcgtggcag ccctttgacc gtggcctgca tctgctggtt ggtgaacagc
     5101 aatggcgcgg ggcccactat gtgcaaagct ggctgcgcgg gctgacacat tacctgctgc
     5161 tggatgagcc ccgtacgcag cgcacagtac tggagccacg caccgataac cagcgcctgt
     5221 tccgccatct tgagcctgcg ggataccgga caattaaaga gttcgacttc ccacataagc
     5281 gctcgcgcat ggtgatggcg gatcgtcatc acttcttcac ggaggtcggt ctgtaatgaa
     5341 tcacaaggac tgggattttg ttaaccgtcg gctggtggca aaaatgttgt ctgagatgga
     5401 gtatgagcag gttttccacg cggaatctca gggcgatgac cactactgca ttaacctgcc
     5461 gggagcacaa tggcgcttca tcgctgaacg tggtatctgg ggctggctgt ggattgatgc
     5521 ccaaacgttg cgctgcacgg acgaaccagt actggcgcag acgctgctga tgcagctaaa
     5581 gccggtgctg tcaatgagtg atgccactgt tgcagagcat atgcaggatt tgtatgccac
     5641 gctactgggc gacctgcaac tgctgaaggc ccgtcgcgga ctgagtgcca gtgacctgat
     5701 taatcttgat gccgatcgtc tgcaatgcct gctgagcggt catcctaaat tcgtttttaa
     5761 taaaggtcgc cgtggctggg gcaaagaggc gctggaacga tatgcgccag agtataccaa
     5821 caccttcaga ctgcactggc tggcggtaaa acgtgaacat atgatctggc gctgcgataa
     5881 cgatctggat attcagcagt tgttgactgc cgcaatggat ccgcaggagt ttacacgctt
     5941 cagtcaggtc tggcaggaaa acgggctgga tcacaactgg ctaccgctgc cggtacatcc
     6001 gtggcagtgg cagcaaaaaa tcgccacaga cttcatcgct gattttgtcg aaggcaggat
     6061 ggtgtctctc ggcgagtttg gcgaccagtg gctcgcccag cagtcgctgc gtaccctgac
     6121 caacgccagc cgccggggag ggctggatat caagctgccg ctgaccatct acaacacctc
     6181 atgctaccgg ggaatccctg gcagatatat cgctgccgga ccactggctt cacgctggct
     6241 gcaacaggtt tttgcgaccg acgccaccct ggtgcaaagc ggcgcagtga tccttggtga
     6301 accggctgca ggctatgtgt cccatgaagg ctatgccgcg cttgcccggg ctccctatcg
     6361 ctaccaggaa atgcttggtg ttatctggcg ggagaatccg tgccgctggc tgaaaccgga
     6421 tgaaagtcct gttctgatgg caacactgat ggagtgcgac gaaaacaatc agccgctggc
     6481 aggcgcatat atcgaccgct ccggactgga cgctgaaacc tggcttacgc aattgttccg
     6541 ggtggttgtg gttccgctgt atcacctgct ttgccgctac ggtgtcgcgc ttattgcaca
     6601 cggacaaaat ataacgctcg ccatgaaaga gggggtacca cagcgtgttc tgctgaaaga
     6661 cttccagggc gatatgcggc tggtgaaaga agcgtttccc gaaatggact ctttgcctca
     6721 ggaggtgcgt gatgccacat cccgtctgag tgcggactac ttaatccatg atttgcagac
     6781 gggtcacttc gtgacagtac tgcgttttat ttcgccactg atggttcgtc ttggcgtacc
     6841 tgaaaggcga ttttatcaac tgctggcagc agtgttgagt gattacatga acaaacatcc
     6901 acaaatggca gagcgttttg cgcttttctc actcttcagg ccacaaatca ttcgcgtggt
     6961 gctgaacccg gtaaaactga cctggccgga cctggatggc ggcagtcgca tgctgccgaa
     7021 ttaccttgag aatctgcaaa atccgctgtg gctggtaact caggaatatg aatcatgaaa
     7081 aaaagtgtcg attttattgg tgtcgggaca gggccattta atctcagcat tgctgcattg
     7141 tcacatcaga tcgaagaact gaactgtctc ttctttgacg aacatcctca tttttcctgg
     7201 catccgggta tgctggtacc ggattgtcat atgcagaccg tctttctgaa agatctggtc
     7261 agtgcagttg cacctacaaa tccctacagc tttgttaact atctggtgaa gcacaaaaag
     7321 ttctatcgct tccttacaag caggctacgt acagtatccc gtgaagagtt ttctgactat
     7381 ctccgctggg ctgctgaaga tatgaataac ctgtatttca gtcataccgt tgaaaatatt
     7441 gatttcgaca aaaaaagtcg attgtttctg gttcagacca gtcggggaga atattttgcc
     7501 cgcaatatct gccttggtac aggaaaacaa ccttatttac caccctgtgt gaagcatgtg
     7561 acacaatcct gtttccatgc cagtgaaatg aatcttcgtc ggccggacct gagtggaaaa
     7621 cggataaccg tggttggtgg aggacagagt ggtgcagacc tgttccttaa tgcattacgc
     7681 ggggaatggg gagaagcggc ggaaataaac tgggtctcac ggcgtaataa ttttaacgca
     7741 ctggatgagg ctgcttttgc tgatgagtat tttacacctg aatatatttc aggcttctcc
     7801 ggactgaagg aagatattcg ccatcagtta ctggatgagc agaaaatgac atcggatggc
     7861 atcactgccg attctttact gaccatttat cgtgagttgt accaccgttt tgaagttctg
     7921 agaaaaccaa gaaatatccg tctgctaccc agtcgctcgg taacaaccct ggaaagtagt
     7981 ggtccgggct ggaggttatt gatggaacat catctggatc agggcagaga gagcctggaa
     8041 agtgatgtgg tgattttcgc cacaggttac cgttctgcat tgccacaaat acttccctca
     8101 ctgatgccct tgatcaccat gcacgataag aacaccttta aagtgcgtga tgacttcact
     8161 ctggaatgga gtggcccgaa agaaaataac atctttgcgg tcaacgccag catgcaaact
     8221 catggcatcg ccgaacccca gctcagcctg atggcctggc gatctgcacg tattcttaat
     8281 cgcgtactgg ggcgtgattt attcgatctc agtatgccgc ccgcactgat tcagtggcgc
     8341 agcggcagcc ggaaaaaacc gcagccggag gctgcttctt taactcacta tacagcaaat
     8401 attcaggaat aacgatgatg cgcaaaaagt atatgccccg ggctcttggt ccgctgcttc
     8461 ttgtcgtgct gtcaccagct gtcgcccagc aaaacgatga taatgagatc atagtgtctg
     8521 ccagccgcag caatcgaact gtagcggaga tggcgcaaac cacctgggtt atcgaaaatg
     8581 ccgaactgga gcagcagatt cagggcggta aagagctgaa agacgcactg gctcagttaa
     8641 tccccggcct tgatgtcagc agccagagtc gaaccaacta cggtatgaac atgcgtggcc
     8701 gcccgctggt tgtcctgatt gacggtgtgc gcctcaactc ttcacgttcc gacagccgac
     8761 aactggactc tgtcgatcct tttaatatcg accatattga agtgatctcc ggcgcgacgg
     8821 ccctgtacgg tggcgggagt accggagggt tgatcaacat cgtgaccaaa aaaggtcagc
     8881 cggaaaccat gatggagttt gaggctggca caaaaagtgg ctttaacagc agtaaagatc
     8941 acgatgagcg cattgccggt gctgtctccg gcggaaatga ccatatctcc ggacgtcttt
     9001 ccgtggcata tcagaaattt ggcggctggt ttgacggtaa cggcgatgcc accctgcttg
     9061 ataacaccca gaccggcctg cagcactcca atcggctgga catcatggga accggtacgc
     9121 tgaacatcga tgaatcccgg cagcttcaac tgataacgca gtactataaa agtcagggag
     9181 acgacaatta cgggcttaat ctcgggaaag gcttttccgc catcagcggg agcagcacac
     9241 catacgtcag taaggggctg aattctgacc gcattcccgg cactgagcgg catttgatca
     9301 gcctgcagta ctctgacagt gatttcctgg gacaggaact ggtcggtcag gtttactacc
     9361 gcgatgagtc gttgcggtac tacccgttcc cgacggtaaa tgcgaataaa caggcgacag
     9421 ctttctcctc gtcacagcag gataccgacc agtacggcat gaaactgact ctgaacagcc
     9481 aactgatgga cggctggcaa atcacctggg ggctggatgc tgagcatgag cgctttacct
     9541 ccaaccagat gttcttcgat ctggctcagg caagtgcttc cggagggctg aacaaccata
     9601 agatttacac caccgggcgc tatccgtcat atgacatcac caatctggcg gccttcctgc
     9661 aatccagcta tgacattaat gatattttta ccgttagcgg tggcgtacgc tatcagtata
     9721 ctgagaacag ggtagatgat ttcatcgact acacgcagca acagaagatt gctgccggga
     9781 aggcgatatc tgccgacgcc attcctggtg gttcggtaga ttacgataac tttctgttca
     9841 atgctggtct gctgatgcac atcaccgaac gtcagcaggc atggttcaat ttttcccagg
     9901 gggtggcatt gccggatccg gggaaatatt atggtcgcgg catctatggt gcagcagtga
     9961 acggccatct ttccctgaca aagagcgtga acgtcagcga cagtaagctg gaaggcgtga
    10021 aagtcgattc ttatgaactg ggctggcgct ttaccggtga caacctgcgg actcaaatcg
    10081 cggcatatta ctcgctttcc aataagagcg tggaaaggaa taaagatctg accatcagtg
    10141 tgaaggacga caggcgccgt atttacggcg tggaaggtgc ggtggactac ctgatcccgg
    10201 atactgactg gagtaccggt gtgaacttca atgtgctgaa aaccgagtcg aaagtgaacg
    10261 gtcaatggca aaaatatgac gtgaaggaat caagtccatc gaaagcgaca gcttacatta
    10321 actgggcgcc ggaaccgtgg agtctgcgtg tacagagcac cacttctttc gacgtaagcg
    10381 atgcagaggg taacgatatt aatggttaca ctaccgtcga ttttatcagt agttggcagc
    10441 ttccggtggg aacactcagc ttcagcgttg agaacctctt cgaccgtgac tataccactg
    10501 tctggggaca gcgtgcacct ctgtactact gcccgggtta cggccctgct tcactgtacg
    10561 actacaaagg ccggggccga acctttggtc tgaactactc agtgctgttc tgacggggat
    10621 gcttttgcaa caagggtatg ctgtaaacaa catggtcctt ggtggccatg ttgtttacag
    10681 ccagtataat atacagcctt aacaagatat tccgtctccg gaatggactg ttcaccactg
    10741 aataacatta tttctgtttt atggatcttt gactgaaatt tatcgcatat cctcatttca
    10801 ttgtccgtct gaaaactaac cttacaagta agcgtcgtct cagcaccgtc tggcagatcc
    10861 tgaaattcct gagaggatga tctgcgccca atatcctgag ccggaacttt gctgcaatga
    10921 aggccaacga aaaatggggg agtatgttat agagtacttg ctacagggta aaaagaggta
    10981 cctgtcgctg gttctccgtt cagtttaacc gggagatttt ctcctgcgcc tgtcataaag
    11041 tccggtaatg gagattgtta atacgatggt gcacgatgca ttgtcggtgc ccggcccgga
    11101 ggatgccccg taactacata cggatcaggg ttggcaatat cgaatggcag gctataggca
    11161 aagttaaagc cccatggagt agcacaaaat atgccacaca aaggaaacgg tctgaataac
    11221 gcagtgatgg agaacttctt cggcacactg caaaatcgca tcgtggtcga tcgctaattc
    11281 gccgtgaatc actgaacgat gttccatgcg aacagatatt cgatcatatc gaaatattaa
    11341 acattacccc tccccgcatt gggcaaaaaa aaaacaaaaa acgatcaatt agtattgatt
    11401 cacataatct ctcatcatta atgagaacat tgtcacatat ttacaatata gaactgttac
    11461 ttttagtaag ctgttagtaa ccaactgtta ctaataattt taattcttaa tactttgttc
    11521 attcaataaa tgagttgaga g
//
LOCUS       DAJTZL010000129        11426 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.129, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000129 DAJTZL010000000
VERSION     DAJTZL010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11426
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.129"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(42..383)
                     /gene="bamE"
                     /locus_tag="OO544_003022"
     CDS             complement(42..383)
                     /gene="bamE"
                     /locus_tag="OO544_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HCR6768554.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(532..2193)
                     /gene="recN"
                     /locus_tag="OO544_003023"
     CDS             complement(532..2193)
                     /gene="recN"
                     /locus_tag="OO544_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HCR6768555.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALEIARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLNKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            complement(2279..3157)
                     /gene="nadK"
                     /locus_tag="OO544_003024"
     CDS             complement(2279..3157)
                     /gene="nadK"
                     /locus_tag="OO544_003024"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HCR6768556.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            3280..3873
                     /gene="grpE"
                     /locus_tag="OO544_003025"
     CDS             3280..3873
                     /gene="grpE"
                     /locus_tag="OO544_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HCR6768557.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            complement(3928..5214)
                     /locus_tag="OO544_003026"
     CDS             complement(3928..5214)
                     /locus_tag="OO544_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCR6768558.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(5235..6026)
                     /locus_tag="OO544_003027"
     CDS             complement(5235..6026)
                     /locus_tag="OO544_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HCR6768559.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            6193..7554
                     /gene="ffh"
                     /locus_tag="OO544_003028"
     CDS             6193..7554
                     /gene="ffh"
                     /locus_tag="OO544_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HCR6768560.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            7803..8051
                     /gene="rpsP"
                     /locus_tag="OO544_003029"
     CDS             7803..8051
                     /gene="rpsP"
                     /locus_tag="OO544_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HCR6768561.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            8070..8618
                     /gene="rimM"
                     /locus_tag="OO544_003030"
     CDS             8070..8618
                     /gene="rimM"
                     /locus_tag="OO544_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HCR6768562.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            8649..9416
                     /gene="trmD"
                     /locus_tag="OO544_003031"
     CDS             8649..9416
                     /gene="trmD"
                     /locus_tag="OO544_003031"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HCR6768563.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            9458..9805
                     /gene="rplS"
                     /locus_tag="OO544_003032"
     CDS             9458..9805
                     /gene="rplS"
                     /locus_tag="OO544_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HCR6768564.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(9882..10364)
                     /locus_tag="OO544_003033"
     CDS             complement(9882..10364)
                     /locus_tag="OO544_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HCR6768565.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAIGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(10380..>11426)
                     /gene="dgcN"
                     /locus_tag="OO544_003034"
     CDS             complement(10380..>11426)
                     /gene="dgcN"
                     /locus_tag="OO544_003034"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HCR6768566.1"
                     /translation="TYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNILAS
                     WHYTRKDPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSLAV
                     LTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFHRF
                     ALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSDARK
                     TSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEVGGLRHKAYRLGGDEFAMVLY
                     DVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIEHASAEKLQELADHN
                     MYQAKHQRAEKLVR"
BASE COUNT         2802 a         2746 c         3061 g         2817 t
ORIGIN      
        1 attgagcacc ttttttaacg tctttgagag caactttatt attagttacc actcagcgca
       61 ggtttgttat caatattggt caacacaccg ctactgttaa aggtcagcgt cagcgtttgc
      121 tgcgttacac cttcatgacc tggttgctgg cggaagacat agaaccaggt attcgtacca
      181 aatggatcgg acatcagcgg tgtacccaat gcgtacgcaa cttgttgttg cgtcatgcca
      241 acacgtattt tggatacgtc gttagcggtc agatagttcc cctggttgat gtcaggacgg
      301 taaaccactc gctccagagt ggaacagcct gcggtcaaca tcaatagtac tgctgctgca
      361 gcagtcagcg ttttacagcg catagtgatt tgattccttt tcgggcccga gcagtacgtg
      421 gctcatctgt aatatgccga tgataataga cctttcatca ctttaaaacc ttttgctttc
      481 cggtcttacg gcgttttgct gtttactcta accgtgaagc aggaaaaaag tttacgctgc
      541 aagcagttct ttcgcattcg ctagtgtatt acgtgtgact tcactgccac caagcaggcg
      601 cgccagctct tgtaaccgcg cttttttatt cagggattgc atatgcgttt ctgtcatcgc
      661 accatcggtt tctttgctga caaaatagtg ttgatgacca catcccgcga cttgtggcag
      721 gtgggtaaca cacatcacct gagttgattc gccgagttga cgcagcagtt tgccgacaac
      781 tgccgctgtt ggaccgctaa tccctacatc cacttcatca aaaatcagtg ccggggtttc
      841 cattttacgc gccgtgatga cctggattgc cagtgcgatg cgggacaatt caccaccgga
      901 tgcgactttg gcaataggct gcattggctg acctgggttg gtggttaccc gaaactcaat
      961 acgatcggca ccgtcagcgc ccaggtgatg ctcgtcaaat ttaacatcga tcgtaaactg
     1021 cccatgcggc attgagagcg catgcatact gtcggtgatc agctgtgcaa gttcttctgc
     1081 atattgctgg cgttgttggt gtaatgcgcg cgcgatttcc agtgcctgct gatgatgttt
     1141 cgttaccgcc agcgcaagcg tttcttgtga gtcggcctga tcgtccagtt gctgctgttc
     1201 ttccagtagc gactggtaat actgtggcaa tgcctcaggg ctgacgtgat gtttacgtgc
     1261 cagcgaaatc tgttttgaga tgcgctgttc aagttcaaat agtcggttgg gatcgagatc
     1321 cagacgatcg cagtagtggc gcagttcatc gctggcttca gcaatctgga tggtagcttc
     1381 ttccagcata tcaagtacgc cggacagttt gctgtccatg ccaatcaatt cgctcaccag
     1441 ttgtttagcc gtgtaaagct gactttgcag gtttgcgtct tcaccgtcgg ccattaatgc
     1501 caatgcattc tggctggtgg tcagcaattg accgctgttc gccagacgtt tgtactcttc
     1561 gtcgatttgt tcaaactctc cgggctgcgg attaaattcg ttaagttctt ttaattggta
     1621 ttgcagcagt tccgcacggg cggcgcgttc ctgacttaac tgttgatgat gcgcgaggtc
     1681 acggcagctt tgatgccaca actgataacg tgcggtcatt tcctgcagta gagaggtttc
     1741 attggcatag ccatcaagca ggaatttttg gtgctcaggt ttggtgagta attgatgagc
     1801 gtgctgacca tggatctgaa tcagcaactg acccagttcg cgcagttgtg acagaggaac
     1861 agctgtaccg ttgatgaaac cacgggagcg accatcgctg ctgatcacgc gacgaagcaa
     1921 acattcatgc ccgtcttcaa gctggttttc ttccagccag cgcagagccg ctggcgtatc
     1981 tttcagagaa aaacgggcgc acaggtcagc gcgagcagcg ccggtacgca ccatgtcggc
     2041 ttcagcgcga ccaccgagac aaagaccgag ggcatctatt gcaatagatt tacccgcgcc
     2101 ggtctcgcca gttattacgg tcatgccgct atgaaaatca atctcaagct cacgaacgat
     2161 agcaaagttg ctgatggtca gttgtgccaa catagtcgct ttcctgtatg aaaaaccatt
     2221 actgttattg tgtacagtat aaactggttt tatatacagt aaagaggctg gcgtaaaatt
     2281 agaataattt ttttgaccag ccgagcttgg tgcttaatgt gttgaaataa ctgtaatctt
     2341 tcggatgaat cagattcaga tggtaatcac agcgacgaat caggacatct tcaccttcct
     2401 gaatcggcag tgctatctgg ctgtcgcaac tgatttccag gtcgttacgg cgatgcgaaa
     2461 aacgcagacg gatcgtgctg ctgctgttta tgaccagtgg tcgtgctgac aacgtatgcg
     2521 ggaacatggg caccagggta atcgcatcca gagagggggt cagaatagga ccgcctgcag
     2581 agagggaata ggcggtggag cctgttggcg tcgaaataat tagtccatca gatcgctgag
     2641 aaaacgcaaa gatctcgtcg atatacactt cgaactcaat catatgcgcc actttgcctg
     2701 gatgaagcac cacttcattt atcgcggtgc tgatgcgttt ctggcaatct tgctgacaga
     2761 cttgcgcttc cagcaaaaaa cgtttctcgc tgatgtagtg gccttccagc acatcggcta
     2821 actgttgctg ggcgttatcg gggtcaaggt cagtcaggaa acccaggttg ccacggttga
     2881 ttccaataac tttaatatcg taacgggcga gtgtgcgtgc cgcgcccagc atattaccgt
     2941 cgccaccaac gactaccgcg agatcagcta gttgcccaat ctccgcgagc gtgccagttt
     3001 tcacattctt cagttgcagt tcgtgagcga tttgttgctc aacgatgacc tcgtaacctt
     3061 ttgtgcacag ccagcggtag agcatttcat gtgttgtcag tgcagtgggg tgccgtgggt
     3121 gtcccacaat gccaatacac ttgaaatgat tattcatttt tccgaggtcc ttgttgcgaa
     3181 gattgatgac aatgtgagtg cttcccttga aaccctgaaa ctgatcccca taataagcga
     3241 agttagcgag atgaatgcga aaaaaacgcg gagaaattca tgagtagtaa agaacagaaa
     3301 acgcctgagg ggcaagcccc ggaagaaatt atcatggatc agcacgaaga gattgaggca
     3361 gttgagccag aagcttctgc tgagcaggtg gatccgcgcg atgaaaaaat tgcgaatctc
     3421 gaagctcagc tggctgaagc ccagacccgt gaacgtgacg gcattttgcg tgtaaaagcc
     3481 gaaatggaaa acctgcgtcg acgtactgaa ctggatattg aaaaagccca caaattcgcg
     3541 ctggagaaat tcatcaacga attgctgccg gtgattgata gcctggatcg tgcgctggaa
     3601 gtggctgata aagctaaccc ggatatgtct gcgatggttg aaggcattga gctgacgctg
     3661 aagtcgatgc tggatgttgt gcgtaagttt ggtgttgaag tgatcgccga aactaacgtg
     3721 ccgctggacc cgaatgtgca tcaggctatc gcaatggtgg aatctgatga cgttgcgcca
     3781 ggtaacgtac tgggcattat gcagaagggt tatacgctga atggtcgtac gattcgtgcg
     3841 gcgatggtaa ctgtagcgaa agcaaaagcg taatttctgt tttcgtaata attcacggcc
     3901 ctgcatgcga atgcagggcc gttttcgtta ctccgccaca ctctcgcgca gcggtttcac
     3961 aggaaaaact tttacctgct taatcatatt gtcctgtacg tcgagaatat ctatatcgta
     4021 ctcgccaata cgcacgcggg tgcctgcgac agggatctct tccagtgcct caagaatgac
     4081 gccattaacc gtgcgggcat catcttccgg tagatgccag ttaaaggctt tgttgatttc
     4141 ccgcacgttg gcggtgccat cgataatcac cgaaccgtcg ttttgcggcg tgacctcttc
     4201 ggcaagtgtt ggcgacatcg acgtagtgaa atcgccgaca atctcttcca gaatatcttc
     4261 aaccgtcacc agcccctgaa tgtctccata ctcgttgacg accaggccga ctttcttttt
     4321 gttgcgctga aactttacca actgcgtgct gagcggcgta ccttccggca caaaatagat
     4381 ctcgtccgcg gcgcgcagca tggtttcttt ggtgaactct tttttctccg acatcaaccg
     4441 ccaggcttca cgtactcgca gcatactgat ggcgtcgtcc agcgaatcac ggtagagcac
     4501 gatgcgcccg tgaggtgagt gggagagttg gcgcagaatc gatttccagt catcgttgat
     4561 atcaataccg ataatttcac tgcgcggcac catgatgtca tcaacggtca ttttttccag
     4621 atcgagcacc gacagcagca tatcctgatt gcgacgggaa atttgtgagc gtgattcgtg
     4681 cacgatagtg cgcaactctt ctttgctcaa agagccgcta accacgatat cggttttgat
     4741 acccatcatg cgcatcagca tacgggtgat agcattcagc aaccagacca gcggcatcat
     4801 caaaatttgc agcggagcca gcagaaaact actcggataa gcgacttttt ccgggtacag
     4861 cgcggcaatg gttttcggca atacctcagc aaataccagt acgacaaaag tcagcacacc
     4921 agtcgcaatt gccacgcccg catcgccgta caaacgcatc ccaacaatag tgccgagcgc
     4981 ggaggcaaga atattgacca ggttattgcc gattaacacc aggcttatca ggcggtctgg
     5041 cttacgcagc aatttttcga cgcgtttggc cgagcgatta ccctgtttcg ccatatgtcg
     5101 cagacgatag cggttgaggg tcatcattcc ggtttcggac ccggaaaaat aggctgaaat
     5161 gaccaccatg atgatcagaa taatgatcaa cgtagtagta gaaatgtgtt ccaggggaaa
     5221 ctccttttct gggtttagct gattaactgc tggacaattc ggctgccgaa gtaggccagt
     5281 gtcagaatta ccgcgcccgc aacgttaaac cagacgacgc ggcgtccacg ccatccttca
     5341 tggtaatgtc cccacagcag cacaatatag acaaaccacg ccacgataga gagcacagcc
     5401 ttgtcgatat tttccatgct aaataggttg tgcatgtaga acaggccagt gcaaagcgtg
     5461 agcgttagca gcaccacgcc aatctgcgtg atgtggaaca ttttacgctc gatactcatc
     5521 aatggcggca tttcctggtt aaatgccagc ttcttgttct tcagttggta atcaatccac
     5581 gccagttgca gcgcgtacag ggcggcgata attagcgtgg cataggaaaa gagcgataag
     5641 ccaatgtgca ccagcatccc aggcgttgct tccagatggg tgatgtattc attgggcatg
     5701 aaggtcgcca gcgccaggtt gataagcgca aaggcataga caatgggtag cagcaaccag
     5761 ccacgattgc gagaagccac aatggtcatt accgtacaga tcatcaaact gaccaatgaa
     5821 ccaacgttca gcaggctgag gttttgtccg ctatcaccgt cgggcaggat gcgggcttcc
     5881 agagcgattg cgtggcagac cagcgcaatg accgcagaaa taatagccat gcgccgccag
     5941 ccgccgtttt tttgcagcag accgggaaca atcagcgcaa gactgacgga gtaggcgaca
     6001 agcgcgagca gagcaaaaac gggcatagtg atgtcgacag tttgagcaat aagaaagaga
     6061 agcagtatag cgtcaggtga acgctgctcc aaccgttgca taacaacaaa gacgccttca
     6121 tgttatactg cggcaaaata ctgatgatgt gtcgcgattg cggccaaccg tttccacccc
     6181 aggcgagaga caatgtttga taatttaacc gatcgtttgt cgcgcacgct gcgcaatatc
     6241 agtggccgtg gacgcctcac tgaagacaac gtaaaagata cgctgcgcga agtgcgcatg
     6301 gcgctgctgg aggcggatgt cgctctgccg gtagtgcgtg agtttatcaa tcgcgtaaaa
     6361 gagaaagcgg ttggtcatga agttaataag agcctgacgc cggggcagga gttcgtcaaa
     6421 atcgtccgta acgaactggt tgcggcgatg ggcgaagaga accagaccct gaacctggcg
     6481 gcgcaaccgc ctgcggtcgt gctgatggcg ggcctgcaag gtgccggtaa aacaaccagc
     6541 gttggtaaac tcggtaagtt cctgcgcgag aagcacaaga agaaagtgct ggtggtttct
     6601 gccgacgttt atcgcccggc ggcaatcaaa cagcttgaga cgctggcaga gcaggtgggc
     6661 gttgatttct tcccttctga tgttggtcag aagccggtag atatcgttaa cgcggcgctg
     6721 aaagaagcca aactgaaatt ctacgacgtg ctgctggtgg ataccgctgg tcgtctgcac
     6781 gttgacgaag cgatgatgga cgagatcaaa caagtccatg cgtcgattaa cccggttgaa
     6841 accctgtttg tggttgacgc catgaccggt caggatgcgg ccaatacggc aaaagcattc
     6901 aatgaagcgt taccgcttac cggcgtagtg ttgaccaaag tggacggcga tgcccgcggc
     6961 ggtgcggcgc tctctattcg tcacatcact ggcaaaccga tcaagtttct cggtgttggc
     7021 gagaaaactg aggcgctgga gccgttccat ccggaccgca tcgcgtcgcg tattctcggc
     7081 atgggcgacg tactgtcgct gatcgaagat atcgaaagca aagttgaccg cgcgcaggca
     7141 gagaaattag ccagcaagct gaaaaaaggc gacggcttcg atctcaacga ttttcttgag
     7201 cagctgcgcc agatgaaaaa tatgggcggc atggctagcc tgatgggcaa gctgccgggc
     7261 atggggcaga tcccggataa tgttaagtcg cagatggacg ataaagtgct ggtgcgtatg
     7321 gaagccatca tcaactcgat gacgatgaaa gagcgcgcta agccagaaat catcaaaggt
     7381 tcgcgtaagc gtcgtatcgc tgccggttgc ggtatgcagg tgcaggacgt taaccgcctt
     7441 ctgaagcagt tcgacgacat gcagcgcatg atgaagaaaa tgaagaaggg cggaatggcg
     7501 aagatgatga gaagcatgaa gggtatgatg cccccaggct tccctggtcg ctaatcggcc
     7561 tggcttgttt gccattttgg caccgggatg tgctcgtcat cctcacgtac tgcgtgtacg
     7621 ctccggtgac tgcgcgcact ccggcaccaa actggctgca ccgccgacgg ccttttacag
     7681 cagagttagc aactgttgat tgcaattccc gcaaaaatga gtaaaatttt cgggctttta
     7741 atatgacacc ggactccgtt cctcgatggg gtccggttgt tttattcaca caagaggatg
     7801 ttatggtaac tattcgttta gcacgtcacg gcgctaaaaa gcgtccgttc taccaggttg
     7861 ttgtcgctga cagccgtaat gcacgcaacg gtcgcttcat cgagcgcgtt ggtttcttca
     7921 acccaatcgc tagcgaaaaa gaagaaggca ctcgcctgga tctggatcgc atcgctcact
     7981 gggttggcca gggcgcaact atttctgatc gcgttgctgc gctgatcaaa gaagtaaaca
     8041 aagcagctta atctgtcacg gtggtcatga tgagcaaaca actcaccgcg caagcacctg
     8101 ttgatcccat cgttttggga aaaatgggtt cgtcttacgg tattcgtggg tggctcagag
     8161 tgttttcttc caccgaagac gccgaaagca tttttgacta tcagccctgg tttatccaga
     8221 aggcgggtca gtggcagcaa gtccagctgg aaagctggaa gcaccacaat caggacatga
     8281 tcatcaagct gaaaggcgtt gacgatcgtg atgcggcgaa cctgctgacg aattgtgaaa
     8341 ttgtcgtgga ttcatcgcag ctgcctcagc ttgaagaggg ggactactac tggaaagacc
     8401 tgatgggctg ccaggtagta accactgaag gctacgatct cggtaaagtc gtcgatatga
     8461 tggaaaccgg atctaatgac gttctcgtca ttaaggcaaa cctgaaagat gcgtttggta
     8521 tcaaggaacg tctcgtaccg ttcctcgatg ggcaggttat caagaaagtc gatctcacta
     8581 ctcgttcaat cgaagtagat tgggatcctg gtttttaaac caccggataa acggtaaaag
     8641 acggcgctat gtggattggc ataattagcc tgtttcctga aatgttccgc gcaattaccg
     8701 attacggggt aactggccgg gcagttaaaa atggcctgct gagcatccag agttggagtc
     8761 ctcgcgactt cacgcatgac cggcaccgta ccgtggacga tcgtccttac ggtggcggac
     8821 cggggatgtt aatgatggtg caacccttgc gggacgccat tcatgcagca aaagccgcgg
     8881 cgggtgaagg cgcaaaggtg atttatctgt caccacaggg acgcaagctt gatcaagcgg
     8941 gcgtcagcga actggcaacg aatcaaaaat tgattctggt gtgcggtcgc tacgaaggta
     9001 tagatgagcg cgtgatccaa accgaaattg acgaagaatg gtcaatcggc gattacgttc
     9061 tcagtggtgg tgagttacca gcaatgacgc tgattgactc cgtttcccgg tttattccgg
     9121 gagtactggg acatgaagcc tcggcaacgg aagattcctt tgctgaagga ttgctggatt
     9181 gcccgcacta tacgcggcct gaggtgttag aagggatgga agttccgcca gtgttactgt
     9241 cgggcaacca tgccgagata cgtcgctggc gtttgaaaca gtcgctgggc cgtacctggc
     9301 ttagaagacc tgaacttctg gaaaacctgg ctctgactga agagcaagca aggttgctgg
     9361 cggagttcaa aacggaacac gcacaacagc aacataaaca tgatgggatg gcgtaagccc
     9421 ccgagatatc agtttaccca ggataagaga ttaaattatg agcaatatta ttaagcaact
     9481 tgaacaagag cagatgaagc aggacgtacc ttccttccgt ccgggtgata ccgtggaagt
     9541 gaaagtatgg gttgttgaag gttccaaaaa acgtctgcag gcattcgagg gcgtggttat
     9601 cgctattcgt aaccgcggtc tgcactctgc attcactgtt cgtaaaattt ccaacggcga
     9661 aggcgttgag cgtgtcttcc agactcactc tccggtagtt gacagcattt ctgtcaaacg
     9721 tcgtggtgct gttcgtaaag ctaaactgta ctacctgcgt gagcgtactg gtaaggctgc
     9781 tcgtatcaaa gagcgtctta actaagattt cgcttaagcg acatcctgtt aagaagggct
     9841 ggccaattgg ctggcccttt ttttgtctgt ttgctcgtgt tttaaggggt tgtaatcacc
     9901 actgcgacgc ggcggttctc ggcgcggcct tgggcggtct tgttactggc tatgggatat
     9961 ttttttccta aaccctgtgt ggtgagattg ctgcgtggaa tttgtccacc aatagcccat
    10021 gcatcggcca cgacattcgc ccgtttcaat gataagcctt cgttgtaact gtcttcacca
    10081 tagttatcgg tgtgtccatc catacgggca tgtgttagcc ctgtcgaggc caatttggct
    10141 gccatggttt ggatctgttg ctggctttcc gggagcaatt tgtagtcatt ttttgcgaac
    10201 agaatggcat ctgataagcc cagcgaccag tcgccggcgg attcagtaaa tccataagat
    10261 tgcatagcgg cgacttgctc aggagtaaac tttccctgag gggactggca gccagtcagt
    10321 attagtgagg tgaaaatcag gggtgctact aggtgcttta tcatatcgat atatccttgt
    10381 tatctcacca gcttttcggc acgctggtgt ttggcctgat acatattgtg atcggcaagc
    10441 tcttgtaatt tttccgcaga ggcgtgctca atggtcatcg cgtaaccaat gcttaatgtc
    10501 atggtggtct gatggccatt atgaagatca aacgggagat taaagatttg tgtcagtgct
    10561 gagcatatct gctgcacttc agattctgac tgtacatcat agagcaccat agcgaattca
    10621 tcgccgccca ggcggtatgc tttatgtcgc agcccgccaa cttcagctaa ccgttttgcg
    10681 atttcaatca agactctatc gcccgtcgca tgaccccagg tatcattgat gtatttgaaa
    10741 ttatcgccat caagaaatag taacgccgac gtttttcggg catcggaatt gttcatcaac
    10801 gtgttgatgc cgctacgaaa cgctgcgcgg ttagccagcc cggttaatgg gtcatgtagc
    10861 gcggtacgta gaagctgcgc atttttagcc tgtaaacgaa gctgccactc ttccatttca
    10921 tccagcagac tgttgaagtc gagagcgaag cggtgaaact cagcgatacg ttcttccgaa
    10981 actcgtcggg aaaaattgcg gttggaacgc acatcatgta cgacatcggt gatatttttc
    11041 agtgcttcca ccaggccatt gtgcaaatgg cgggtgaggg taattgcgat gcctgatgcc
    11101 agcagaatac aaccggtcag tacggcgagc gaaaaccaga taaaatggct gattgaactg
    11161 tcgcgagcgg ttaagcgtac ttcgccaatg gtttcaccat tgtgacgaat cggctgaatg
    11221 atgggggcgg ggaagagcca gtggcttatg aaattgctga aagtatcgcc tggatcctta
    11281 cgggtgtaat gccaggacgc cagaatattt tgctgcttat cacgtacttc tgcagttgaa
    11341 aattgccctt gctggcccag cgctgccagt gtttcagttg ctgcagggcc atcggcaaaa
    11401 acgacagctg cttccagact gtaagt
//
LOCUS       DAJTZL010000130        11357 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.130, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000130 DAJTZL010000000
VERSION     DAJTZL010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11357
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.130"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..537)
                     /gene="espL1"
                     /locus_tag="OO544_003035"
     CDS             complement(<1..537)
                     /gene="espL1"
                     /locus_tag="OO544_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
                     /protein_id="HCR6768567.1"
                     /translation="MSQNDIIIRTHYKSPHRLHIDSDIPTPSSEPINQFARQLITLLD
                     TSDLSSMLSYCVTQEFTANCRKISQNCYSTALFIINFATSPIHAENILITLHYKKEII
                     PLFLETTPIKANHLRSILDYIEQEQLTAEDRNHCMKLSKKIHREKTIQPTVNLNGSAF
                     FSQSPSDAIFCRHLSLQYA"
     gene            1062..2990
                     /gene="thrS"
                     /locus_tag="OO544_003036"
     CDS             1062..2990
                     /gene="thrS"
                     /locus_tag="OO544_003036"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HCR6768568.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            2994..3536
                     /gene="infC"
                     /locus_tag="OO544_003037"
     CDS             2994..3536
                     /gene="infC"
                     /locus_tag="OO544_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HCR6768569.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            3633..3830
                     /gene="rpmI"
                     /locus_tag="OO544_003038"
     CDS             3633..3830
                     /gene="rpmI"
                     /locus_tag="OO544_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HCR6768570.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            3883..4239
                     /gene="rplT"
                     /locus_tag="OO544_003039"
     CDS             3883..4239
                     /gene="rplT"
                     /locus_tag="OO544_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HCR6768571.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     misc_feature    4356..4480
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            4362..4406
                     /gene="pheM"
                     /locus_tag="OO544_003040"
     CDS             4362..4406
                     /gene="pheM"
                     /locus_tag="OO544_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HCR6768572.1"
                     /translation="MNAAIFRFFFYFST"
     gene            4689..5672
                     /gene="pheS"
                     /locus_tag="OO544_003041"
     CDS             4689..5672
                     /gene="pheS"
                     /locus_tag="OO544_003041"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HCR6768573.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLLGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DTTRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            5687..8074
                     /gene="pheT"
                     /locus_tag="OO544_003042"
     CDS             5687..8074
                     /gene="pheT"
                     /locus_tag="OO544_003042"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HCR6768574.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            8079..8378
                     /gene="ihfA"
                     /locus_tag="OO544_003043"
     CDS             8079..8378
                     /gene="ihfA"
                     /locus_tag="OO544_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HCR6768575.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            8479..9459
                     /gene="btuC"
                     /locus_tag="OO544_003044"
     CDS             8479..9459
                     /gene="btuC"
                     /locus_tag="OO544_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HCR6768576.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASAVLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            9522..10073
                     /gene="btuE"
                     /locus_tag="OO544_003045"
     CDS             9522..10073
                     /gene="btuE"
                     /locus_tag="OO544_003045"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HCR6768577.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPENPIVMESIKLALAK"
     gene            10073..10822
                     /gene="btuD"
                     /locus_tag="OO544_003046"
     CDS             10073..10822
                     /gene="btuD"
                     /locus_tag="OO544_003046"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HCR6768578.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            10900..>11357
                     /locus_tag="OO544_003047"
     CDS             10900..>11357
                     /locus_tag="OO544_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HCR6768579.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKEQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARR"
BASE COUNT         2872 a         2631 c         3058 g         2796 t
ORIGIN      
        1 ggcgtattgc aatgacagat ggcgacaaaa aatagcgtca gaaggagatt gcgaaaaaaa
       61 tgcactacca ttgagattta ctgttggttg tatagttttt tctctatgga tttttttaga
      121 cagtttcata caatggttac gatcttcggc agttaactgt tcctgttcaa tataatccag
      181 tatgcttcgc aaatggttag ccttaatagg cgtggtttcc agaaataagg gaatgatttc
      241 ttttttatag tgtaatgtaa tgagtatatt ttctgcatgg atgggtgaag tggcaaagtt
      301 aatgataaaa agggcagtgg aataacaatt ttgtgaaatt tttcgacagt ttgcggtaaa
      361 ttcctgagta acacagtatg acagcatcga acttaagtca gaggtatcaa gtagggtgat
      421 gagttggcgc gcaaattgat taataggctc tgatgaaggc gttggtatgt cgctatcgat
      481 gtgcaatcta tgaggagact tataatgggt tctgataatg atatcgtttt gactcataat
      541 ctattcgcct tatatatgtc tggaggtttt gaggatgatg attaattatt atcgccagaa
      601 cgaaatgtgc acgaaaaatt gctataagaa ttgtgtcact tttatcattg tgatatgacc
      661 acttcgtcat taatctcatg agcgtttaaa atactaccgc caaatcacgc aacgaacttt
      721 gctggattca gtttatgctg ctgtaaatcc gctcgcagta aacctttcag acgcaccgtg
      781 atgttatcag tggttcttta agcgttttgc tggtgtactc actacaaacg aattgcgaat
      841 cgaatcaatg tgaaacggaa aggtacaatc tccctttttt tagtgttaac atcgttcaac
      901 cgggtggaag gtgagaggtt aatgtctttc atttgtgtac ctgattgcga accaatttag
      961 catttgttgg ctagatggtt tcgcaatgaa ctgttaataa acaaattttt ctttgtatgt
     1021 gatctttcgt gtgggtcacc actgcaaatt aaggatataa aatgcctgtt ataactcttc
     1081 ctgatggcag ccaacgccat tacgaccacg ctgtaagccc catggatgtt gcgctggaca
     1141 ttggtccagg tctggcgaaa gcctgtatcg cagggcgcgt taatggcgaa ctggttgatg
     1201 cttgcgatct gattgaaaac gacgcacaac tgtcgatcat taccgccaaa gacgaagaag
     1261 gtctggagat cattcgtcac tcctgtgcgc acctgttagg gcatgcgatt aaacaacttt
     1321 ggccgcatac caaaatggca atcggcccgg ttattgacaa cggtttttat tacgacgttg
     1381 atcttgaccg cacgttaacc caggaagatg tcgaagcact cgagaagcgg atgcatgagc
     1441 ttgctgagaa aaactacgac gtcattaaga agaaagtcag ctggcacgaa gcgcgtgaaa
     1501 ctttcgccaa ccgtggggag agctacaaag tctccattct tgacgaaaac atcgcccatg
     1561 atgacaagcc aggtctgtac ttccatgaag aatatgtcga tatgtgccgc ggtccgcacg
     1621 taccgaacat gcgtttctgc catcatttca aactaatgaa aacggcaggg gcttactggc
     1681 gtggcgacag caacaacaaa atgttgcaac gtatttacgg tacggcgtgg gcagacaaaa
     1741 aagcacttaa cgcttacctg cagcgcctgg aagaagccgc gaaacgcgac caccgtaaaa
     1801 tcggtaaaca gctcgacctg taccatatgc aggaagaagc gccgggtatg gtattctggc
     1861 acaacgacgg ctggaccatc ttccgtgaac tggaagtgtt tgttcgttct aaactgaaag
     1921 agtaccagta tcaggaagtt aaaggtccgt tcatgatgga ccgtgtcctg tgggaaaaaa
     1981 ccggtcactg ggacaactac aaagatgcaa tgttcaccac atcttctgag aaccgtgaat
     2041 actgcattaa gccgatgaac tgcccgggtc acgtacaaat tttcaaccag gggctgaagt
     2101 cttatcgcga tctgccgctg cgtatggccg agtttggtag ctgccaccgt aacgagccgt
     2161 caggttcgct gcatggcctg atgcgcgtgc gtggatttac ccaggatgac gcgcatatct
     2221 tctgtactga agaacaaatt cgcgatgaag ttaacggatg tatccgttta gtctatgata
     2281 tgtacagcac ttttggcttc gagaagatcg tcgtcaaact ctccactcgt cctgaaaaac
     2341 gtattggcag cgacgaaatg tgggatcgtg ctgaggcgga cctggcggtt gcgctggaag
     2401 aaaacaacat cccgtttgaa tatcaactgg gtgaaggcgc tttctacggt ccgaaaattg
     2461 aatttaccct gtatgactgc ctcgatcgtg catggcagtg cggtacagta cagctggact
     2521 tctccttgcc gtctcgtctg agcgcctcct atgtgggcga agacaacgag cgtaaggtac
     2581 cggtaatgat tcaccgcgca attctggggt cgatggaacg tttcatcggt atcctgaccg
     2641 aagaattcgc tggtttcttc ccgacctggc ttgcgccagt tcaggttgtt atcatgaata
     2701 ttaccgattc acagtctgaa tacgttaacg aattgacgca aaaactatca aatgcgggca
     2761 ttcgtgttaa agcagacttg agaaatgaga agattggctt taaaatccgc gagcacactt
     2821 tgcgtcgcgt cccatatatg ttggtctgtg gtgataaaga ggtggaatca ggcaaagttg
     2881 ccgttcgcac ccgccgtggt aaagacctgg gaagcatgga cgtaaatgaa gtgatcgaga
     2941 agctgcaaca agagattcgc agccgcagtc ttaaacaatt ggaggaataa ggtattaaag
     3001 gcggaaaacg agttcaaacg gcgcgcccta accgtatcaa tggcgaaatt cgcgcccagg
     3061 aagttcgctt aacaggtctg gaaggcgagc agcttggtat tgtgagtctg agagaagctc
     3121 tggagaaagc agaagaagcc ggagtagact tagtcgagat cagccctaac gccgagccgc
     3181 cggtttgtcg tataatggat tacggcaaat tcctctatga aaagagcaag tcttctaagg
     3241 aacagaagaa aaagcaaaaa gttatccagg ttaaggaaat taaattccgt cctggtacag
     3301 atgaaggcga ctatcaggta aaactccgca gcctgattcg ctttctcgaa gagggtgata
     3361 aagccaaaat cacgctgcgt ttccgcggtc gtgagatggc gcaccagcaa atcggtatgg
     3421 aagtgcttaa tcgcgtgaaa gacgatttgc aagaactggc agtggtcgaa tccttcccaa
     3481 cgaagatcga aggccgccag atgatcatgg tgctcgctcc taagaagaaa cagtaaggcc
     3541 ttcaagtagc actgtctgtg gagccttcgg gcttcacagg ctttgttcgc ctttgtttcg
     3601 tttattaaca atgcgaagtg gaagttatta aaatgccaaa aattaagacc gtacgcggtg
     3661 ctgctaagcg cttcaaaaaa accggtaaag gtggttttaa gcacaagcac gctaacctgc
     3721 gtcacattct gaccaaaaaa gcgaccaaac gtaaacgtca cctgcgtccg aaagccatgg
     3781 tttccaaagg cgatctgggc ctggtaatcg cgtgcctgcc gtacgcataa gccgttaacg
     3841 tttttaactt tttaattaga atatagatac aggagagcac atatggctcg cgtaaaacgt
     3901 ggtgttattg cacgcgcacg tcacaagaaa attttgaaac aagctaaagg ctactacggt
     3961 gcgcgttctc gcgtataccg cgttgccttc caggctgtta tcaaagctgg tcagtatgct
     4021 taccgtgacc gtcgtcaacg taagcgtcag ttccgtcaac tgtggattgc gcgtatcaac
     4081 gcagcagcac gtcagaacgg tatttcttac agcaaattca tcaatggcct gaaaaaagcc
     4141 tctgttgaaa tcgaccgtaa gatcctggct gatatcgcag tattcgacaa agtagcgttc
     4201 accgctctgg ttgaaaaagc gaaagcagct ctggcataag ccagttgaaa gagggagcta
     4261 gtctccctct tttcgtttca acgccatcaa aacattgact tttatcgccg tagccttttc
     4321 aataaaggtc ttttgaagag taccaaaagg taacgcaagc aatgaatgct gctattttcc
     4381 gcttcttttt ttactttagc acctgaatcc aggaggctag cgcgtgagaa gagaaacgga
     4441 aaacagcgcc tgaaagcctc ccagtggagg ctttttttgt atgcgcgttt tgaaaaataa
     4501 aaagccagtc tatcaggcgt aattgtcgca gccagtctgg acacggccag cgcggagaaa
     4561 ccggagcgta tttaagtacg tgagaatttc gagcactgcc cgggaccaaa atggcaagta
     4621 aaatagcctg atgggatagg ctctaagtcc aacgaaccag tgtcaccact gacacaatga
     4681 ggaaaaccat gtcacatctc gcagaactgg ttgccagtgc gaaggcggcc attagccagg
     4741 cgtcagatgt tgccgcgtta gataatgtgc gcgtcgaata tttgggtaaa aaagggcact
     4801 taacccttca gatgacgacc ctgcgtgagc tgccgccaga agagcgtccg gcagctggtg
     4861 cggttatcaa cgaagcgaaa gagcaggttc agcaggcgct gaatgcgcgt aaagcggaac
     4921 tggaaagcgc agcgctgaat gcgcgtctgg cggcggaaac gattgatgtc tccctgctag
     4981 gtcgtcgcat tgaaaacggc ggtctgcatc cggttacccg taccatcgac cgtatcgaaa
     5041 gtttcttcgg tgagcttggc tttaccgtgg caaccgggcc ggaaatcgaa gacgattatc
     5101 ataacttcga tgctctgaac attcctggtc accacccggc gcgcgctgac cacgacactt
     5161 tctggtttga cactacccgc ctgctgcgta cccagacctc tggcgtacag atccgcacca
     5221 tgaaagccca gcagccaccg attcgtatca tcgcgcctgg ccgcgtttat cgtaacgact
     5281 acgaccagac tcacacgccg atgttccatc agatggaagg tctgattgtt gataccaaca
     5341 tcagctttac caacctgaaa ggcactctgc acgatttcct gcgtaacttc tttgaggaag
     5401 atttgcagat tcgcttccgt ccttcctact tcccgttcac cgagccttct gcagaagttg
     5461 acgtcatggg taaaaacggt aaatggctgg aagtgctggg ttgcgggatg gtgcatccga
     5521 acgtgctgcg taacgttggc atcgacccgg aagtttactc tggtttcgcc ttcgggatgg
     5581 ggatggagcg tctgactatg ttgcgttacg gcgtcaccga cctgcgttca ttcttcgaaa
     5641 acgatctgcg tttcctcaaa cagtttaaat aaggcaggaa tagattatga aattcagtga
     5701 actgtggtta cgcgaatggg tgaacccggc gattgatagc gatgcgctgg cgaatcaaat
     5761 cactatggcg ggcctggaag ttgacggtgt agaaccggtt gccggtagct tccacggcgt
     5821 ggtcgttggt gaagtggttg agtgtgcgca gcatccgaac gctgacaaac tgcgtgtgac
     5881 aaaagtgaat gtcggcggcg atcgcctgct ggacatcgtc tgtggtgcgc caaactgccg
     5941 tcagggcctg cgtgtggcgg tagctaccat tggtgctgtt ctgccgggtg atttcaaaat
     6001 taaagcggcg aaactgcgtg gcgaaccgtc tgaagggatg ctgtgctcct tctctgaact
     6061 gggaatttct gacgatcaca gcggcattat cgaactgcct gcagatgcgc cgattggcac
     6121 tgacatccgc gaatacctga aactcgatga caacaccatc gaaatcagcg taacgccaaa
     6181 ccgtgccgac tgcttaggta tcattggtgt tgcgcgtgac gttgccgtgc tgaatcagct
     6241 gccgctggtt gaaccggaaa tcgttccggt tggtgcgacc atcgacgaca cgttgccgat
     6301 tacagtcgaa gcgcctgaag cctgcccgcg ctatcttggc cgtgtggtaa aaggcattaa
     6361 cgttaaagcg ccaactccgc tgtggatgaa agaaaaactg cgtcgttgcg ggatccgttc
     6421 tatcgatgca gttgttgacg tcaccaacta tgtactgctc gaactgggcc agccgatgca
     6481 cgctttcgat aaagatcgca ttgaaggcgg cattgtggtg cggatggcaa aagagggcga
     6541 aacgctggtg ctgcttgatg gcactgaagc gaagctgaat gctgacactc tggtcatcgc
     6601 cgaccacaac aaggcgctgg cgatgggcgg catctttggt ggcgaacact ctggcgtgaa
     6661 tgacgaaacg caaaacgtct tgctggaatg cgctttcttc agcccgctgt ctatcaccgg
     6721 tcgtgctcgt cgtcatggcc tgcatactga tgcgtctcac cgttatgagc gtggcgttga
     6781 tccggcactg cagcacaaag cgatggaacg tgcgacccgt ctgctgatcg acatctgcgg
     6841 tggtgaggct ggtccggtaa ttgatatcac caacgaagca acgctgccga agcgtgcaac
     6901 catcacttta cgtcgtagca aactggatcg cctgatcggc catcatattg ctgatgaaca
     6961 ggtaactgac attctgcgtc gtctcggctg cgaagtgacc gaaggcaaag acgagtggca
     7021 ggcagttgcg ccgagctggc gtttcgatat ggagattgaa gaagatctgg ttgaagaagt
     7081 cgcgcgtgtt tacggctaca acaacatccc ggatgagccg gtacaggcaa gcctgattat
     7141 gggtactcac cgtgaagctg acctgtcgct caagcgcgtg aaaacgctgc tcaacgacaa
     7201 aggctatcag gaagtgatca cctacagctt cgttgatccg aaagtgcagc agatgatcca
     7261 tccaggcgtt gaagccttac tgctgccaag cccgatctct gttgaaatgt cagcaatgcg
     7321 tctttctctg tggaccggcc tgctggcaac cgtggtgtac aaccagaacc gtcagcagaa
     7381 ccgtgtgcgc attttcgaaa gcggtctgcg tttcgtacca gatactcagg caccgttggg
     7441 cattcgtcag gatctgatgt tagccggtgt gatttgcggt aaccgttacg aagagcactg
     7501 gaacctggca aaagagaccg ttgatttcta tgatttgaaa ggcgatcttg aatccgttct
     7561 cgacctgacc ggtaaactga atgaggttga gttccgtgca gaagcgaatc cggcactgca
     7621 tccggggcaa tccgcagcga tttatctgaa aggggaacgt attggttttg ttggagttgt
     7681 tcatcctgaa ctggaacgta aactggatct taacggtcgc actctggtgt tcgaactgga
     7741 gtggaacaag ctcgcagacc gcgtggtgcc tcaggcgcgc gagatttctc gcttcccggc
     7801 gaaccgtcgt gacatcgcgg tggtggtcgc agaaaacgtt cccgcagcgg atattttatc
     7861 cgaatgtaag aaagttggcg taaatcaggt agttggcgta aacttatttg acgtgtaccg
     7921 cggtaagggt gttgcggagg ggtataagag cctcgccata agcctgatcc tgcaagatac
     7981 cagccgtaca ctcgaagaag aggagattgc cgctaccgtc gccaaatgtg tagaggcatt
     8041 aaaagagcga ttccaggcat cattgaggga ttgaacctat ggcgcttaca aaagctgaaa
     8101 tgtcagaata tctgtttgat aagcttgggc ttagcaagcg ggatgccaaa gaactggttg
     8161 aactgttttt cgaagagatc cgtcgcgctc tggaaaacgg cgaacaggtg aaactctctg
     8221 gttttggtaa cttcgatctg cgtgataaga atcaacgccc gggacgtaac ccgaaaacgg
     8281 gcgaggatat tcccattaca gcacggcgcg tggtgacctt cagacccggg cagaagttaa
     8341 aaagccgggt cgaaaacgct tcgcccaaag acgagtaatc tgatctaact aaaaaggccg
     8401 ctctgcggcc ttttttcttt tcactgtcga agagtcaccg taaaatcaac gccatgacac
     8461 ttcagcagaa cggataccat gctgacactt gcccgccaac aacagcgaca aaatattcgc
     8521 tggttattat gcctgtcagt tttgatgctg ctggcgcttc tcttaagcct ttgcgccggt
     8581 gaacaatgga tttcgccagg tgactggttt actcctcgtg gcgaactgtt cgtctggcaa
     8641 attcgcctgc cacgtacgct ggctgtattg ctggttggtg cggcgctggc tatatccggc
     8701 gctgtaatgc aggcgttgtt tgaaaatcct ctggcagaac ctggactact tggcgtctct
     8761 aacggcgcag gcgtggggct tatcgccgcg gtattgcttg ggcaagggca actccccaac
     8821 tgggcgctag ggctgtgtgc gattgctggc gcgcttatca tcactttaat actcttacgt
     8881 ttcgcccgtc gtcatctttc gaccagtcgg ttattgctgg ctggcgttgc attagggatt
     8941 atctgtagcg cactaatgac gtgggctatc tacttttcca cctctgttga tttacgtcag
     9001 ctgatgtact ggatgatggg cggttttggc ggcgtagact ggcggcaaag ctggctgatg
     9061 ctggcattga tccccgtgtt gttgtggatc tgttgtcagt ccaggccgat gaatatgtta
     9121 gcacttggcg agatctcggc gcggcaactg ggtttacccc tgtggttctg gcgcaatgtg
     9181 ctggtggcag cgaccggctg gatggttggc gtcagtgtgg cgctggcggg tgctatcggc
     9241 tttattggtc tggtgatccc acatattctt cggttgtgtg gtttaaccga tcatcgcgta
     9301 ttacttcccg gctgcgcgct ggcaggggcg agcgcagtgc tgctggccga tattgtagcg
     9361 cgcctggcat tagctgccgc agagctgcct attggcgtgg tcaccgcaac gttgggtgcg
     9421 ccggtgttta tctggttatt gttaaaagca ggacgttagc cgcaaaaaga cggtctatga
     9481 ttaaaagcta agatttttac tgaccacacc caggagaaac gatgcaagat tccattctga
     9541 cgaccgtagt gaaagatatc gacggtgaag tgaccacgct ggagaagtac gccggtaatg
     9601 tgctgttgat tgtcaatgtc gcctcaaagt gtggcttaac gccgcaatat gagcagttgg
     9661 agaatattca gaaagcctgg gccgatcgag gttttgtggt gctgggattc ccgtgcaacc
     9721 agtttctgga acaagaaccg ggcagcgatg aagagattaa aacttactgt accaccacat
     9781 ggggggtgac gttcccgatg ttcagtaaga ttgaagttaa cggcgaagga cgccatccgc
     9841 tgtatcaaaa attgattgcc gcagcgccga ccgcagtcgc gccggaagag agcggattct
     9901 atgcccgtat ggtcagcaaa ggtcgtgcac cgctgtaccc tgatgatatt ttatggaatt
     9961 ttgagaaatt tttagttggc agggacggat tggtcatcca gcgtttttcc ccggatatga
    10021 cgccggaaaa ccccattgtg atggaaagca ttaaactggc gttggcaaaa taatgtctat
    10081 tgtgatgcag ttacaggatg ttgcggaatc tacccgccta gggccacttt ctggcgaggt
    10141 tcgggctggg gagatcctgc acctggtggg gccgaatggc gcgggtaaga gtaccttact
    10201 ggcacgaatg gccggaatga ccagcggtaa gggaagtatt cagttcgcgg ggcaaccact
    10261 ggaagcatgg tccgcaacaa aactcgcgct gcatcgcgcc tatctttcac aacagcagac
    10321 gccgccgttt gcaatgccgg tctggcacta tctgacactg catcagcacg ataaaacgcg
    10381 taccgaacta ctgaatgatg tcgcaggggc gctggctctt gatgacaaac tcggacgtag
    10441 caccaatcaa ctttccggcg gtgaatggca acgcgtacgt cttgctgcgg tggtgctgca
    10501 aatcacacca caagccaatc ccgcaggcca attgctgctt cttgatgagc cgatgaacag
    10561 tcttgatgtt gcgcaacaaa gtgcgttaga caaaattctg agcgctctgt gtcagcaagg
    10621 actggcgatt gtgatgagca gtcacgatct caaccacaca ttgcgtcatg cgcatcgggc
    10681 gtggttgcta aaaggtggaa aaatgctggc cagtggacgc agagaagagg tgctcacgcc
    10741 gccaaatctg gcgcaggcct atgggatgaa ttttcgccgt ctggatatcg aaggtcacag
    10801 aatgctgatt tcgaccatct gatttcatta gtatcttgaa aatcgggtaa ttacgacgct
    10861 aaattaatgc aagaataaaa aacagaggaa ccgtacggaa tgcgtttctg ccttattttg
    10921 atcacagcac tgtttctggc cgggtgtagc caccataaag caccgccgcc aaatgccaga
    10981 ctttctgatt cgattaccgt tattgccggt ttgaacgacc agctacaaag ctggcatggc
    11041 acgccgtatc gttatggtgg catgacgcgg cgcggtgtgg actgttcggg atttgtggtt
    11101 gtaacgatgc gcgatcgttt cgatttgcag ctgccccgag aaaccaaaga acaagcctct
    11161 atcggcacgc aaattgataa agacgagttg ctgcctggtg acctggtctt tttcaaaacg
    11221 ggttccggac aaaatggttt gcatgtaggt atttacgata ccaacaacca atttatccac
    11281 gcctctacca gcaagggagt gatgcgttcc tcacttgata atgtctattg gcagaaaaat
    11341 ttctggcagg cgagacg
//
LOCUS       DAJTZL010000131        11270 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.131, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000131 DAJTZL010000000
VERSION     DAJTZL010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11270
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.131"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..686)
                     /gene="hpaI"
                     /locus_tag="OO544_003048"
     CDS             complement(<1..686)
                     /gene="hpaI"
                     /locus_tag="OO544_003048"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HCR6768580.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGAGFDWLLIDGE
                     HTPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     EAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGAL"
     gene            complement(697..1500)
                     /gene="hpaH"
                     /locus_tag="OO544_003049"
     CDS             complement(697..1500)
                     /gene="hpaH"
                     /locus_tag="OO544_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hepta-3-ene-1,7-dioic acid hydratase"
                     /protein_id="HCR6768581.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAEGRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLEAGQIILGGSFTRPVPARKGDTFHVDYGNMGSISCRFV"
     gene            complement(1611..1991)
                     /locus_tag="OO544_003050"
     CDS             complement(1611..1991)
                     /locus_tag="OO544_003050"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HCR6768582.1"
                     /translation="MPHFIVKCSDNIREEADLPGLFAKVNPTLAATGIFPLAGIRSRV
                     HWVDTWQMADGQRDYAFVHMTLKIGAGRSLESRQQAGEMLFELIKTHFAALMESRLLA
                     LSFEIEELHPTLNFKQNNVHALFK"
     gene            complement(2001..2852)
                     /gene="hpaD"
                     /locus_tag="OO544_003051"
     CDS             complement(2001..2852)
                     /gene="hpaD"
                     /locus_tag="OO544_003051"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HCR6768583.1"
                     /translation="MGKLALAAKITHVPSMYHSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     ADEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            complement(2854..4320)
                     /gene="hpaE"
                     /locus_tag="OO544_003052"
     CDS             complement(2854..4320)
                     /gene="hpaE"
                     /locus_tag="OO544_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HCR6768584.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWANLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALVSPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADSLGELALEADIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVGALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            complement(4317..5606)
                     /locus_tag="OO544_003053"
     CDS             complement(4317..5606)
                     /locus_tag="OO544_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HCR6768585.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALANDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPGDRVRVLAEGFPPLENPVVDEREVTTHKSFPTQ
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEDTAK"
     gene            5878..6324
                     /gene="hpaR"
                     /locus_tag="OO544_003054"
     CDS             5878..6324
                     /gene="hpaR"
                     /locus_tag="OO544_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HCR6768586.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEAIPGDNE"
     gene            6443..8098
                     /gene="tsr"
                     /locus_tag="OO544_003055"
                     /pseudo
     CDS             6443..8098
                     /gene="tsr"
                     /locus_tag="OO544_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418775.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
     gene            complement(8147..9498)
                     /locus_tag="OO544_003056"
                     /pseudo
     CDS             complement(8147..9498)
                     /locus_tag="OO544_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418776.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(9713..10627)
                     /locus_tag="OO544_003057"
     CDS             complement(9713..10627)
                     /locus_tag="OO544_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR6768587.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSHLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPGETFSELQLARAAGVSPVVVREYLLKFGRYNLIHSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            10766..>11270
                     /gene="lgoD"
                     /locus_tag="OO544_003058"
     CDS             10766..>11270
                     /gene="lgoD"
                     /locus_tag="OO544_003058"
                     /EC_number="1.1.1.414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HCR6768588.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVGLGKNIADLKNGQQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLV"
BASE COUNT         2580 a         2925 c         3075 g         2690 t
ORIGIN      
        1 aacagcgcgc ccagttccag atagcgtttt gccagttgct cattggcgat caggatcccc
       61 ggcgctttgc ccgcttcgcg gatctgcacg atcgcctgct caatggcggc ctgtacttcc
      121 gggtgctgcg gattaccggc ataacccata tcggcgctca gatccgccgg gccgataaac
      181 acgccgtcga cgccttccac gtccagaatc tgcggtaagt tcttcattgc ctcacgcgtt
      241 tcgatctgca ccagcacgca catttgatcg ttggcttttt gcaggtaatc aggaatacga
      301 ttccagcgcg aggcgcgggc cagcgcactg cccacaccgc gaataccggc ggggggataa
      361 cgggtggcgc gtaccgcttc acgggcttcg tcagcgtttt gtaccatcgg caccagcaag
      421 gtttgtgtgc cgacgtccag cagttgtttg atttgcaccg gatcgttcca cgacggacgt
      481 actaccggct ggctggggta gggcgcaatc gcctgtagct gggtgagcac ggtttgcacg
      541 ttattcggcg tgtgctcacc gtcgatcaac aaccagtcga atcctgctcc ggccagtaac
      601 tctgcgctgt agctgctact cagccccagc cataatccga tctgcggacg gcctgctttc
      661 agcgccgctt taaagctgtt ttccatcgtt ctctccttaa acaaagcggc agctaatgga
      721 acccatgttg ccgtaatcga cgtggaaggt gtcgccctta cgcgccggaa ccgggcgggt
      781 gaacgaacca ccgagaatga tttgcccggc ttccagttgt acgtcatacg gtgcgagttt
      841 gttcgccagc caggccacgc cgtttgccgg atgattcagc acgccagcgg cgacgccggt
      901 ttcttcaatc acgccattgc gatacatcag ggcggagatc caacgcagat ccagttcgtc
      961 tggtttgatc ggacgaccgc cgaggatcac cccggcgttg gcggcgttat cagaaatggt
     1021 gtcgaacact ttgcgcgggc gctgggtttc cggatcgatg ttgtggcagc gagcgtcgat
     1081 cagctccagc gccgggatca cgtagtccgt ggcgttgtag acgtcgaaca gcgtgcagtt
     1141 tggcccacgc agcggttttg ccagcacgaa agccagctcc acttcaatgc gcggcacgat
     1201 aaagcgatcg gtcgggatat cgctgccatc gtggaagaac atgtcatcca gcagcgcgcc
     1261 gtaatccggt tcgctgatct gcgagcttgc ctgcatcgct ttcgaggtca ggccgatttt
     1321 gtggcctttc agcgtgcgac cttcggcgat tttcaggcga acccattcac gctgcaccgc
     1381 gtaagcgtct tcgatggtga tctccgggta atccagcgag atcgcgcgga tctgttcgcg
     1441 ctgtttttct gcctgatcca gacgctgggc gatcagggtg tgggtgtgtt tatcgaacat
     1501 agcgatatcc tgtggcattg ccggatgcgg ctgcgcctta tccggcctac ggtgtacggt
     1561 ttggggtttt gtaggcccgg taagcgcagc gccaccgggc aatctgcgcg ctacttaaac
     1621 aatgcgtgca cgttgttttg tttaaaattc agcgtcggat gcagctcttc aatctcaaac
     1681 gacaacgcca gcaggcggct ctccatcagg gcggcgaagt gcgttttaat cagttcaaac
     1741 agcatttcac ccgcctgctg gcggctttcc aggctgcgac ctgcgccgat tttcaacgtc
     1801 atatgcacga aggcataatc acgctgcccg tcggccatct gccaggtatc gacccaatgc
     1861 acgcggctgc gaatacccgc cagcggaaaa atacccgtgg ctgccagcgt cggattcact
     1921 ttggcgaaca accccggcag gtcggcttct tcgcggatgt tgtcactgca tttaacgata
     1981 aagtgcggca taaaacgtcc ttacgcggga agcgggaaaa cagcgttaac ctgaccggtg
     2041 ccagagcttg ggaatagctc ggtgataaac tccaccttgc cgtcgtattt atcccagccg
     2101 agcatcccca gcagcatcac cgtgtcgtgc atattgcctt cgccgtagca gtagtcggcg
     2161 tactccggca gcatattgca gaactctttg aactggcctt cgcgccacaa cttcaccacg
     2221 cgctcgtcca tctggcggtc gaactcgcgg gtgtagctgt tcatcccttc ttccgcacgc
     2281 tgatcgtcaa taaagcggtg cgataacgaa ccgctggcaa ggaccgccac ggtgccgtcg
     2341 tactgttcga tcgctttcag aatcgcttcg cccagcttgc ggctgtcggc aaaatcgtga
     2401 accgtgcaga aagccgaaat ggagaccact ttgaagtgct tgtcttcatt catgtagcgc
     2461 atcggcacca gcgtgccgta ttccagtttc aggctgggaa tgttgtgcgc ttttgcccgc
     2521 acgccgagct ttaaggcttc atcggcaata agctgcccca actccgggtt gccctcgtag
     2581 ttgtaggtca tatcgcggat aaaatgcggc agctcgttgc tggtgtagac gccttcaaaa
     2641 tggtctgcac agttgatgtg ataagcactg ttgaccagcc agtgggtatc gaaaacgata
     2701 atggtatcga cgcccatttc ccggcaacgc ttgctgatct ctttatgccc gtcgatcgcg
     2761 ccctggcggc aaccgtggtt tttccctggc agttcagagt gatacatcga cggtacgtgc
     2821 gtgatcttgg cggctaacgc taacttaccc atatcagact ccccatttcg gaattggatg
     2881 gtcgcccatg gaaatgcaga cgttcttcat ttccgcgaac acttcgaagc tgtactcacc
     2941 gccttcacgc ccggtgccgg aggcttttac gccgccaaat ggctggcgca ggtcacgcac
     3001 gttctgggtg ttgacgaaca ccatgccagc ttcaatgcca cgcgccaggc gtaacacttt
     3061 gctgacatcc tgtgtccaga tgtacgacgc gaggccgtac tccacgtcgt ttgccagacg
     3121 taagccttcg gcttcgtctt taaacggcag caggcaggcg accggcccga aaatctcttc
     3181 ctgggcgact cgcatacggt tatcaacgtc tgccagcacg gttgggcgca ggaagttgcc
     3241 gcctttcagg tgcgcaggca ggtcggacgg tttatccggg ccgcccgcca gcagggttgc
     3301 gccttcttca atgccgagac ggatatagcc ggagactttt tcccagtgct gctggctaat
     3361 aagcgcgcca acctgggtat tcggatcgtt cggatcgccc acgcgcagac ggttggcgcg
     3421 ttcggcaaag cgtttaacga attccgggta gatgctttgc tgaataaaga tgcgcgaacc
     3481 ggcggtgcag cgttcgccgt tgatcgagaa gatggtgaac agggcggcgt ccagcgcgcg
     3541 ttcaatatcg gcatcttcaa aaatcagcac cggcgattta ccgcccagtt ccatggagta
     3601 ttttttcagc ccggcgtttt tcatgatgtt gcgcccggtg gccgtaccgc cggtgaacga
     3661 cacggcacgt acgtcatgat gacgaaccag cgcatcccct gcggttgcgc cgtagccctg
     3721 taccacgttc agcacgcctg ccggaatatc ggcttccagc gccagctcac ccaggctgtc
     3781 agcggtcagc ggggagagtt ccgacatttt cagtaccgcg gtattgccca gcgccagaca
     3841 cggcgcgacc ttccatgtgg cggtcataaa cggtacgttc cacggcgata ccagcgcaca
     3901 aacgcccacc ggctgcacca gcgtgtagtt gagcatcttg tcgtcaaccg gataggtctt
     3961 gccgttcatc tgctggcaga cttccgcgaa aaattcaaag ttgtgggaag cgcgtgggat
     4021 caacacattt ttggtctgat ggatcggcag gccggtgtcc gcggtttcca tcgcggcgat
     4081 ctctggcacg ttctggtcga tcagatcgcc cagacggcgc atcaggcgcg cacgctcttt
     4141 catcggcaga ttggcccatt tcgggaacgc ctctttcgct gccgctaccg cctgattgat
     4201 ctccgcttca ccgccagagg ccacatccgc cagcacttca cccgttgccg gattggtggt
     4261 ctggaagtag tcgttacctg caacattttt gccgttgatc caatgattta cttttttcat
     4321 ttcgctgtat cctcactcac aattcggttc accaggcggc ccacgccttc tacttccacc
     4381 actacttcat cgccaggcac cacgtcagat aagcctttgg gtgtgccggt ggcgatcatg
     4441 tcgcccggat tcagggtcat aaattcactt aagtaggcga tcaggaaggg cacgctgaag
     4501 atcagatcgg cggtggtgcc ttgctggcgt aactcgccgt tgacgaaggt gcgaagggtc
     4561 agattatgcg ggtccgggat cgcctctttc ggcacgatgg ttgaaagcat cggcgtcagt
     4621 ccgtcgcggc tttttacccg caggttaggg cggtagtagt tttccagata gtcacgaatg
     4681 gcgtagtcgt tacacacggt gtagcccgcg acataatcca tggcatcggc ttcgctgacg
     4741 ttacgcgcct gtttgccaat aactaccacc agctccgctt catagtgcat gtattcaata
     4801 ttgtttggac gcacggaggt ctggttatcg ccagtgaggg tatttggcgc tttcaggaac
     4861 accagcggtt cttccggtgg cttaaattcc agttcgctgg cgtggtcggc gtagttcagg
     4921 ccgagggcaa acagcgtgcc gtgcgggtgt ggctgcgttg ggaagctctt gtgcgtggtc
     4981 acttcacgtt cgtccactac cggattttcc agcggcggga aaccttctgc gagaacacga
     5041 acgcgatcgc ctggctgtat ttccacgcgc gcctgtggcg tgccgagcag aatggcgtcg
     5101 cctggattca gcgtggcaaa ttcgctcagg gcgctcagca actgcgcggc gttacgttgt
     5161 aaatcggcgg tattccagtg atcggcagga cgcccgttga tctcggtata gatggtcaga
     5221 ttatcgacat tgctgagagc cacggtttcg ccaatggggc agaatccatc acgacatttt
     5281 gctttgattg ccgggcggta aaagctctct tccggcaggc tgacgtcgtt agccagcgca
     5341 tatccggcga tgtactctgc cgcatcttct tcacgtactt tcgtcgccgt ttttcccaca
     5401 atcagcgcaa cagtcgcacc gctcagtacc ttttcaccct gtggaaaggg aatcggttca
     5461 ccgcaaccaa tcaccgtatt gcgcggttta ataaaccaga ccgcagtttt aggcggggct
     5521 ttgtaggggg attgctggaa cgcttcctgc catgcatcaa gttggctgcg atggttcaac
     5581 gctacggcga agatagtgcc tttcattacc actcctcgga ttcgatcatc ttaatcatta
     5641 atatgttaat gattgctttt attcccgatt gcgtagtttg gcaaaagtaa gtgatgtaaa
     5701 tgtgatctaa ttaacaaact atttacattt aaagataata aaatattaaa tatcagtaag
     5761 ttaataaaat atcatcaatt ttgataaaat caggaattgc aggggcagcg cagtcccggt
     5821 aacgtgacga ttaactgttt ctatattaat gattttgaat ttttcagggg aaagataatg
     5881 cacgactcac taaccattgc gttgctccag gcgcgagaag cggcgatgag ttattttcgc
     5941 ccaatcgtta aacggcataa tttgactgag caacagtggc gcatcgtgcg tattctggcg
     6001 gaaagcccat caatggattt tcacgatctg gcgtatcgcg cctgcatttt gcgcccaagc
     6061 ctgaccggga tcctcacgcg gatggaacgc gacggtttag tgttgcgatt aaagccgatc
     6121 aatgatcagc ggaagctcta tatctcgcta accaaagagg ggcaggcgct gtataaccgc
     6181 gctcagacac agattgaaga agcctatcgg cagattgaag ctcagtttac tgccgagaaa
     6241 atgcaacagt taacccatct gttagaggaa tttattgctc tgggcaattc ccgacaagaa
     6301 gccatcccgg gcgataatga ataacttttt agtatctgat aaccagcgcc tggtttttcc
     6361 atggatggtg ggttacacct tttcataaag tttttgcttt ccaggccgaa aatcttgcat
     6421 cggtccacag gaaagagaaa ccatgttaaa acgtatcaag atcgtgacca gcttactgct
     6481 ggttttggcc gtttttggcc ttttacaact gacatcaggc ggtctgttct ttaatgcctt
     6541 aaagaatgac aaagaaaatt tcactgtttt acaaaccatt cgccagcagc aatccacgct
     6601 gaatggcagc tgggtcgcgt tgttgcagac gcgtaacacc ctcaaccgcg cgggtatccg
     6661 ctacatgatg gatcagaaca atattggtag cggttcaacc gttgctgagc tgatgcagag
     6721 tgccagtatt tcgctgaaac aggcggaaaa aaactgggcg gattacgaag cgttgccgcg
     6781 tgacccgcgt cagagcaccg ccgcagcggc agagatcaaa cgtaattacg atatttatca
     6841 caatgcgctg gcggagctga tccaactgtt aggtgcaggc aaaatcaacg agttctttga
     6901 tcagccgacc cagggatatc aggacggttt cgagaagcag tatgtggctt acatggagca
     6961 aaacgatcgg ctctatgata tcgccgtcag cgataacaat gcctcctaca gccaggcgat
     7021 gtggattctg gtgggcgtga tgatcgtcgt actggcggtc atcttcgccg tctggttcgg
     7081 tattaaagcc tcgctggtag cgccaatgaa tcgcctgatt gacagcattc gtcatattgc
     7141 aggcggcgat ctggtgaaac cgattgaggt ggatggctct aatgagatgg ggcaactggc
     7201 agagagtttg cgccatatgc agggagagct gatgcgtacc gtcggtgatg tgcgcaacgg
     7261 ggccaatgcc atctatagcg gtgccagcga aatcgccacc ggcaataacg atctctcttc
     7321 gcgcaccgag caacaggccg cttcgctgga agagacggca gccagcatgg agtaactgac
     7381 cgcaacggtg aagcagaacg ccgaaaatgc gcgccaggcc agccatctgg cgttaagtgc
     7441 ttctgaaacg gcgcaacgcg gcggcaaagt ggtagataac gtggtgcaga ctatgcgcga
     7501 tatctccacc agttcgcaga aaatcgccga tattatcagc gtaattgacg gcattgcctt
     7561 ccagaccaat attctggctt tgaacgcggc ggttgaggct gcgcgtgcgg gtgagcaagg
     7621 gcgcggtttt gcggtggtcg cgggagaagt gcgtaatctg gcccagcgca gcgcccaggc
     7681 ggctcgtgaa attaaaagcc tgattgaaga ctcggtgggg aaagtggatg ttggctctac
     7741 gctggtcgaa agcgccgggg aaacaatggc ggagattgtc agcgccgtga cccgcgtgac
     7801 ggacattatg ggcgaaattg cttctgcttc tgatgagcag agccgtggta tcgatcaggt
     7861 tggcttagcg gttgctgaga tggaccgggt aactcaacag aacgccgcgc tggtggaaga
     7921 gtctgccgct gccgccgccg cgctggaaga gcaggccagt cgcctgaccg aagcagtggc
     7981 agtgttccgg attcagcaac agcagcgtga aacatcggct gtggtaaaaa ccgtgacgcc
     8041 agctgcgccg cgtaaaatgg ccgtggcaga tagcgaggag aactgggaaa cattttaatc
     8101 gccatgaaaa tgcccgataa gcaaaatgtt atcgggcata aggagattaa tctttacgtg
     8161 ggtcgttgat cggctgacga accaggaaga tgtacgccag tgcccccgct gcggtgacgc
     8221 aaccgcagat gattagtgcc agacggaatg agtgggtggt atcaacaata aaaccagtaa
     8281 tgatcggcgc aaaagaggcg cagatgaagc tggcaaagtt ctggatactg cccaccgacg
     8341 cagtcatgcg agaagcaact gcgacgtgga tcaagcccca gcaggatgtt gaatacagaa
     8401 cagtgccatg ccaatcagca gaaccgctgt catggatgtt gtcgcttgcg gtacgaccag
     8461 cgtaaaggcg gcagaacaga acatcccggc aataatgcag atcttacggc ttttaatcgg
     8521 agccattccc cctttgacca gccagtcagt aacgtaaccg ttgaccagca tcccggcagc
     8581 cccaaacagg aaagggatag ccgccatcaa ccctgtgctt tttaaatcca ggttataggc
     8641 tgtttgcagg taaccaggaa gccaggccag atacagccac gcagtgtagt tgatgccact
     8701 gaatccgagc atcattcccc acattgtacg gttacggaac aggctgcgcc attcggcaaa
     8761 actgagcgga tctcggcggg cattgacgct acctgcattg agataagctt gttcaacggc
     8821 agtcagttct acgtgctcgc ggttgcgata gagcatatac cagccgatgg cgagaaaaat
     8881 ccccagtaca ccaatggtaa taaacatccc gcgccagccc atcaccagca tcatcgccgc
     8941 cagaatcggt gggcttacgg caacgccaat ggtagaagct gcgttgaaga agcccatcgg
     9001 gcgtccgcgc tctttgatgt tgaaccagtc gttaatgact tttacaccgc atgggttcat
     9061 cggcgcttca ccaatcccca taccgatacg caccaacacg aactgcgtaa agttgtgcac
     9121 catgccagac attgcctgga acagtgacca gaagaacatc cccagtccca gcatcaggcg
     9181 tgggccttta cgatccaata gtgggccgca aggaagttgc gcaatcccgt aagcgagtga
     9241 aaacacggag agcaaagcgc cgatttcggt ggcacttaat cccaattctt cacgaatcgt
     9301 taaatttgct accgacagcg aactgcggtc gagataattg attaccgccg caaaaaataa
     9361 taacaacatg gcggtggttt gaatgcgttt aattcgggta ctgcgttgaa ctaatccatc
     9421 tggtggcacg ggaatatctg ccgacgaaga tggagtaaat gaagaacgcg gatcgagggt
     9481 gatattttct ttttccacgc cggactcctg tatttataat tttaattgtt tacgtaattg
     9541 tgaattacgc aaggttgaca ttaacggcag gttaaagttt taggacttta ctaccgctca
     9601 caaaaacatt tttagtcgac tgattgtgag cataggtaag cgggaatttc ccggtattta
     9661 tcttttgata aaaccgtgca cccgctaata gtttgctgtt tttaatccgt acttaatgag
     9721 cataacgcgt gttctcatta atggatcgaa tcattgattg tttagctgaa ttcaaatggt
     9781 tacggagcgc aagggtggca tcaaggtcac tgcggcaaat cagagcgctg agaatggtca
     9841 tatgctcatc gacggcaatg atattgcgct gtttaagatc gctttcgtcc cattgatagt
     9901 gaaaatgaaa gatgactgag atgatctcaa gcgattgatc aaaaaagata ttgtcggcag
     9961 ctgaaagcag taaggagtga aaatctctgt caagttgcga gaacatgcga aagctattgc
    10021 cgatgttgtc acgcagtagc cgatgacgtt cgagcatggt tttcgcctgc agccagcggg
    10081 gatcatgatc aggcaggttg agaaagtgct gaagtgaatg tgtttcgagc atttcgcgta
    10141 gctcaaacag ctgctcggcg taggattgat cgaattgctt catgctccac tggccgcgct
    10201 tttcgctgtg aataagatta taacggccga attttaaaag atattctctt actacaaccg
    10261 ggctgacacc cgcggcccga gccagttgca gttcagaaaa cgtttcccct gggcgtaact
    10321 ggcgctggtt tatcatcgtg aaaaaagcct gctcaaacac tttgttttgt tcactcattg
    10381 aagcggtagt acaggcgaaa ccgtcatcat ggtcaggttt acgcgcaata acatagtcgt
    10441 ttccaacctg cgtcaagacg ccgcattcgc gtaagtggct gagaatatga cgcactgtgg
    10501 tgcggctgat gttgtacatt tccgccagcg cactttgcga tggaagtggg gaagggatat
    10561 gaccccgcgc catatcatca atgacctggt taatcacatt gtggcgtaaa ttttgtgaac
    10621 gactcatatt ttcctccttg tgagtccatt aaaacccgat ttaaaacatt tttatgcgtg
    10681 tgttttcaca aaacagattt tccgttccgc tgccattttt cattttcatt gatatctaag
    10741 gcaacaaaaa atggagagcc gaatcatgtc tacgatgaat gttttaattt gccagcagcc
    10801 gaaagaatta gtctggaaac aacgcgagat acctattccg ggtgacaatg aagcattaat
    10861 aaaaattaag tctgtcggga tttgcggtac cgatattcat gcctggggtg gaaatcaacc
    10921 attttttagt tatccacgtg ttttaggcca tgaaatatgt ggggagattg ttgggctggg
    10981 taaaaatatt gctgatctta aaaatggtca gcaagttgct gtgatccctt atgttgcctg
    11041 tcagcaatgc ccggcgtgta aaagcgggcg taccaattgc tgtgaaaaaa tttcagtcat
    11101 tggcgtgcat caggatggcg gttttagtga gtatttgagc gtgccggtgg cgaacatttt
    11161 gcccgcagac ggtattgacc cgcaggcggc agcattgatt gaacctttcg ctattagcgc
    11221 tcatgcggtg cgtcgcgcag ccattgctcc cggcgagcag gtgctggtgg
//
LOCUS       DAJTZL010000132        11245 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.132, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000132 DAJTZL010000000
VERSION     DAJTZL010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11245
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.132"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..308
                     /locus_tag="OO544_003059"
     CDS             <1..308
                     /locus_tag="OO544_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCR6768589.1"
                     /translation="VLNGKINGNACIVVPLSTTRDHDKLNRGMHVEIASNVINDLQFF
                     DQQIRWAKADLVQQVSRNRLNRARTYRGYLNQCLPHELVADIQRAVIKSINAISLIN"
     gene            507..788
                     /gene="ppiC"
                     /locus_tag="OO544_003060"
     CDS             507..788
                     /gene="ppiC"
                     /locus_tag="OO544_003060"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HCR6768590.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(875..2350)
                     /gene="ilvC"
                     /locus_tag="OO544_003061"
     CDS             complement(875..2350)
                     /gene="ilvC"
                     /locus_tag="OO544_003061"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HCR6768591.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRCHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            2500..3393
                     /gene="ilvY"
                     /locus_tag="OO544_003062"
     CDS             2500..3393
                     /gene="ilvY"
                     /locus_tag="OO544_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HCR6768592.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILDRSDEKTPFELGVCA
                     QKKRLHEPLIEAFWRILPNHK"
     gene            complement(3445..4989)
                     /gene="ilvA"
                     /locus_tag="OO544_003063"
     CDS             complement(3445..4989)
                     /gene="ilvA"
                     /locus_tag="OO544_003063"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HCR6768593.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(4992..6842)
                     /gene="ilvD"
                     /locus_tag="OO544_003064"
     CDS             complement(4992..6842)
                     /gene="ilvD"
                     /locus_tag="OO544_003064"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HCR6768594.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSEQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(6907..7836)
                     /gene="ilvE"
                     /locus_tag="OO544_003065"
     CDS             complement(6907..7836)
                     /gene="ilvE"
                     /locus_tag="OO544_003065"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HCR6768595.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKCIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(7856..8119)
                     /gene="ilvM"
                     /locus_tag="OO544_003066"
     CDS             complement(7856..8119)
                     /gene="ilvM"
                     /locus_tag="OO544_003066"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HCR6768596.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(8116..9762)
                     /locus_tag="OO544_003067"
     CDS             complement(8116..9762)
                     /locus_tag="OO544_003067"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HCR6768597.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVACSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGIKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQYC
                     AQLRDEHSWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(9765..9815)
                     /locus_tag="OO544_003068"
     CDS             complement(9765..9815)
                     /locus_tag="OO544_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6768598.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(9902..10000)
                     /gene="ilvL"
                     /locus_tag="OO544_003069"
     CDS             complement(9902..10000)
                     /gene="ilvL"
                     /locus_tag="OO544_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HCR6768599.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            10353..11245
                     /locus_tag="OO544_003070"
                     /pseudo
     CDS             10353..11245
                     /locus_tag="OO544_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
BASE COUNT         2554 a         3091 c         2951 g         2649 t
ORIGIN      
        1 tcgtgctcaa cggaaaaatt aatggcaacg catgtatcgt cgttccatta tcaaccactc
       61 gcgatcatga caagttaaat cgtggaatgc atgttgaaat agccagtaat gttatcaatg
      121 acctgcaatt ctttgatcag cagattcgtt gggccaaagc cgacctagta caacaagtca
      181 gtcgaaaccg attaaacaga gcaaggactt acagagggta tcttaatcaa tgtttgccac
      241 atgagttggt tgcagacata cagagggcgg tcatcaagtc aatcaatgcc atatcgctca
      301 taaattgaca ataaaataac catccccgat aatgactttg tagcccatag aggccctaag
      361 ttttctcccc ttgagggacc tgcgaagccc ggattttccg ggcttttgcc atatatgtac
      421 cagcccctcc ctgactaaca attacgttag catctgcgga tcatgctaaa atcgccgcct
      481 gacaattatt cactcaaggc acgatcatgg caaaaacagc agcagcactg catatccttg
      541 taaaagaaga gaaactggct ctggatcttc tcgagcagat taagaacggt gccgatttcg
      601 gcaagctggc gaagaaacac tccatttgcc catcaggcaa acgcggcggt gatttaggtg
      661 aattccgcca gggtcagatg gttccggcgt ttgataaagt tgtgttctct tgtccggtac
      721 tggagccaac cggcccgctg cacacccagt tcggttacca catcattaag gtgctgtacc
      781 gtaactaata gcaaggcctt ctccaggaga aggccttgag tgttttctcc ctctccctgt
      841 gggagagggt cggggtgagg gcatcagcgc gcacttaacc cgcaactgca atacgtttca
      901 tatccgtcat atagccgcgc agtttcttac ctacctgctc aatcgcatgg cagcgaatcg
      961 cttcgttcac atcacgcagt tgcgcgttat ctaccgcacc ttccggaata gctttaccca
     1021 gatcgcccgg ttgcagctct gccataaacg gtttcagcag cggcacacaa gcgtaagaga
     1081 acagatagtt accgtactcg gcggtatcag agataaccac gttcatttcg tacagacgct
     1141 tacgggcgat ggtgttggca atcagcggca gttcgtgcag tgattcatag taagcagatt
     1201 cttcgatgat gccggaatca accatggttt caaacgccag ctcaacgccc gctttcacca
     1261 tcgcgatcat cagtacgcct ttatcgaagt actcctgctc gccgattttg ccttcatact
     1321 gcggcgcggt ttcgaaggcg gttttgccgg tctcttcacg ccaggtcagc agtttcttat
     1381 catcgttggc ccagtccgcc atcataccgg aggagaattc gccggagatg atgtcgtcca
     1441 tatgtttctg gaacaggggt gccatgatct ctttcagctg ttcagacagc gcgtaagcac
     1501 gcagtttcgc cgggttagag agacggtcca tcatcagggt gatgccgccc tgcttcagcg
     1561 cttcggtgat ggtttcccaa ccgaactgaa ttagtttttc tgcgtatgcc ggatcggtgc
     1621 cctcttccac cagcttgtcg aagcacagca gagagccagc ctgcaacata ccgcacagga
     1681 tggtttgctc acccatcagg tcagatttca cttccgcaac gaaggacgat tccagcacgc
     1741 ccgcacggtg accaccggtt gctgccgccc atgctttagc gatcgccatg ccttcgcctt
     1801 tcggatcgtt ttccgggtga acggcaatca gcgtcggtac gccgaatcca cgtttgtact
     1861 cttcacgtac ttcggtgcca gggcatttcg gcgcaaccat gactacggta atgtctttac
     1921 ggatctgctc gcccacttcg acgatgttga aaccgtgcga gtaacccagc gccgcgccgt
     1981 ctttcatcag tggctgtacg gtgcgcacta catcagagtg ctgcttgtct ggcgtcaggt
     2041 taaccaccag atccgcctgc gggatcagtt cttcgtaagt accaacttta aaaccgtttt
     2101 cggtcgcttt acgccaggac gcgcgcttct cggcaatcgc ttctttacgc agagcgtagg
     2161 agatatcgag accagaatca cgcatgttca ggccctggtt cagcccctgt gcgccacagc
     2221 cgacgataac cactttttta ccctgaaggt agctcgcgcc gtcggcgaat tcgtcgcgcc
     2281 ccataaagcg acatttgccc agctgtgcca gctgctggcg caggttcagt gtattgaagt
     2341 agttagccat ggtgattcct cgtgatgttg tgcttcttat tgttcggttc gcgtttgcga
     2401 gattgaattc actatatgac aggaaattta ttgcggaaat tgatatattc acaacgtcac
     2461 attgcaattt ctgcaacgtc aacatcgagg gctgcgccat tggatttacg cgatctgaaa
     2521 accttcctgc atctggcgga aagccgccat tttggccgca gcgcgcgggc gatgcacgtt
     2581 agcccatcca cgctctcacg gcagattcag cgcctggaag aagatcttgg tcagccgcta
     2641 tttgtgcgcg ataaccgcac ggtaacgctg acggaagcgg gcgaagagct gcgcgttttc
     2701 gcccagcaaa cgctgttgca gtatcagcaa ttgcgccaca ccatcgatca gcaagggccg
     2761 tcgctctctg gcgaattaca tatcttctgc tcggtgaccg ctgcctacag ccatctgccg
     2821 ccgattctgg atcgcttccg cgcggaacac ccgtcggtgg agatcaaact caccactggt
     2881 gatgcggcag atgcaatgga aaaagtggtc acgggagaag cggatctggc gattgccggg
     2941 aaaccggaga cgctacccgg cgctgtggcg ttttcgatgc tggagaatct ggcagtagtg
     3001 ctgattgccc ccgcgctgcc ctgcccggtg cgtaatcagg tatctgtcga aaagcccgac
     3061 tggtcaacgg tgccgtttat tatggccgat caggggccgg tacgccgccg cattgaactg
     3121 tggtttcgac gcaataaaat cagcaacccg atgatttacg ccacggttgg cgggcatgaa
     3181 gcgatggtat cgatggtggc gctcggctgt ggtgtggcgc tgttgccgga agtggtgctg
     3241 gaaaacagcc cggagcctgt acgcaaccgg gtaatgattt tagatcgcag cgatgagaaa
     3301 acgccgtttg aactgggcgt gtgtgcacaa aaaaagcggc tgcatgagcc gctgattgag
     3361 gcgttctgga ggattttgcc gaaccacaaa tgacgttgtc gcgcgggtag gcctgataag
     3421 cgaagcgcta tcaggcattt ttccctaacc cgccaaaaag aacctgaacg ccggattatt
     3481 ggtttcgtcg tggcaatcgt agcccagctc atttagccgg gtttcgaaat ccggttcatg
     3541 gtcgccgagt tcgaacgccg ccagtacgcg cccgtagtcg gtgccatggc tgcgatagtg
     3601 gaacaaagaa atgttccagt gcgtacccag cgtgttgagg aagcgcagca gcgcgcccgg
     3661 tgattccggg aattcgaagc tgtagaggcg ttcctgcaac ggatgtgacg gacgcccgcc
     3721 gaccatatag cgcacatgca acttcgccat ttcgtcgtcg gagagatcca ccacgctgta
     3781 gccgccgtcg ttgagcatct gcaaaatttc tttgcgctct tcgaggccgc ggctcaggcg
     3841 cacgccgaca aagatgcagg cgtttttggc atcggcaaaa cggtagttga actcggtgac
     3901 cgatcgcccg ccaagcagtt ggcagaattt gaggaagctg cctttttctt ccggaatggt
     3961 caccgccaac aacgcttcac gctgttcgcc cagttcgcag cgttccgaga cgtagcgcag
     4021 gccgtggaag ttcacgttgg caccggaaag aatatgcgcc aggcgttcgc cgcgaatgtt
     4081 gtgctgagcg atatattttt tcattcccgc cagcgccaac gcgccagagg gttccgccac
     4141 tgcgcgcaca tcttcgaata aatccttcat cgccgcacag atcgcatcac tatcgacggt
     4201 gatgatgtcg tcgagatact cctggcataa acggaaggtt tcgtcgccga tgcgttttac
     4261 cgcgacacct tcggcgaaca gtcccacgcg cggcagatca accggatgac ccgcatccag
     4321 cgctgctttc aggcaggcgg agtcttccgc ttctacggcg atcactttga tttgtggcat
     4381 cagttgtttg atcagcaccg caacgcccgc cgccagaccg ccgccgccga ctggcacaaa
     4441 tacgcggtcg agatgggcgt cctgctggag cagttccagc gccagcgtgc cttgcccggc
     4501 aatcaccatc ggatggtcga acggcggcac ccaggtgaac ccctgctgct gtgacagttc
     4561 gatcgcttta gctttcgctt cgtcaaagtt cgcgccatga agcagcactt cgccgccgaa
     4621 tccacgtacc gcatcgactt tgatgtcggc ggtggcggtt ggcataacga tcagggcctt
     4681 cacgcctaac cgcgcagaag aaaacgcgac gccctgcgcg tggttacccg cagaagcagt
     4741 gatcacgccg tgcgcttttt gttcttccgt caggcccgcc atcatggcgt atgcgccgcg
     4801 cagcttaaag ctgtgcactg gctggcgatc ttcgcgcttc accagaatga cgttatcaag
     4861 acgcgacgac agtttttcca ttttttgtag cggcgtaacc tgcgccgcct cgtaaaccgg
     4921 cgcgcgcagc actgctctta aatattcggc accttccgga gcaccggaca ggggttgcga
     4981 gtcagccatt attaaccccc cagtttcgat ttatcgcgca ccgcaccttt gtcggcgctg
     5041 gttgccaggc tggcgtaggc gcgcagggca aaggaaacct gacgttcacg gtttttcggc
     5101 gtccaggctt tgtcaccccg agcttcctgc gcttcacgac gcgccgccag ttcggcatcg
     5161 cttacctgta actgaatgcc acggttcggg atgtcgatag cgatcaggtc accatcttca
     5221 atcagaccaa tgctgccgcc gcttgccgct tccggtgaga cgtggccgat ggaaagacca
     5281 gaggtgccgc cagagaaacg accgtcggtg atcagcgcac aggctttacc gagacccatt
     5341 gatttcagga agctggttgg gtagagcatt tcctgcatgc cagggccgcc tttcgggcct
     5401 tcatagcgga tcaccaccac atcgcccgcg acaactttgc caccgaggat cgcttctacc
     5461 gcgtcgtcct ggctttcgta cactttcgcc gggccggtga atttgaggat gctgtcatcg
     5521 acgcccgctg ttttcacgat gcagccgttt tcagcaaaat taccgtagag caccgccagg
     5581 ccgccgtctt tgctgtaggc gtgttccagc gagcggatac agccgtttgc gcgatcgtca
     5641 tcgagagaat cccaacggca atcctgcgag aatgcctgtg tagtacgaat gcccgcaggg
     5701 cctgcgcgga acatattttt taccgcgtca tcctgggtca gcataacgtc gtattgttcc
     5761 agagtttgcg gcaacgtcag gccaagtacg tttttcacat cacggttcag taaccccgcg
     5821 cgatccagtt caccgagaat accgataaca ccaccagcac ggtgaacatc ttccatatgg
     5881 tatttttggg tgctcggcgc aactttgcat aactgcggga ctttacgcga aagtttgtcg
     5941 atatcactca tggtgaagtc gatttccgct tcctgcgccg ccgccagcag gtgaagtacc
     6001 gtgttggtag agccgcccat cgcgatatcc agcgtcatgg cattttcaaa cgccgctttg
     6061 ctagcaatat tgcgcggcag tgcactttcg tcgttttgct cgtagtaacg tttggtcaat
     6121 tcaacaatgc gtttaccggc attgaggaac aactgcttac ggtccgcgtg ggttgccagc
     6181 agcgagccgt tgcctggctg cgacaaaccc agcgcttcgg tcaggcagtt cattgagtta
     6241 gcggtaaaca tcccggagca ggaaccgcag gttgggcagg cggaacgttc aacctgatcg
     6301 ctctgggagt cagaaacttt cgggtctgcg ccctggatca tcgcatcaac cagatcgagc
     6361 ttgatgatct gctcggacag tttggttttc ccggcttcca tcgggccgcc ggaaacaaag
     6421 atcaccggaa tattcaggcg cagggaagcc atcagcatcc ccggggtgat tttgtcgcag
     6481 ttagagatgc agaccatggc gtcggcgcag tgggcgttga ccatatactc aacggaatca
     6541 gcgatcagtt cgcgagatgg cagtgaataa agcatccccc cgtggcccat agcaatccca
     6601 tcatccaccg caatggtgtt gaactctttg gcaacgccgc cagccgcttc aatttgttcg
     6661 gcgaccagtt taccgagatc gcgcagatgg acgtgacccg gtacaaattg ggtgaacgag
     6721 ttcacaaccg cgataatcgg cttgccaaaa tcggcatcgg tcattccggt ggcgcgccac
     6781 agcgcacgag cacccgccat attacgacca tgagtggtgg tggcggaacg gtacttaggc
     6841 atactttatt tactcccagt gtctgtctcg taaatgggac ggtaagtgcc gtcccatttt
     6901 ttgtatttat tgattaactt gatctaacca gccccattta tcttcggttt cgccagtgaa
     6961 gaggccgaag aaggcttgct gaatgcattt ggtaaccggg ccgcaacggc cttcacccac
     7021 ctgaataccg tctacactgc gcactggcgt gatttctgcc gccgtaccgg acataaacac
     7081 ttcatccgcc aggtacaggg attcgcgcga cagcacctgc tcacgcactt caattcccag
     7141 ctctttcgcc agtttgatga tggcatcacg ggtaataccc ggcagcgcgg atgaggtaaa
     7201 cggtggggtg aacagcacgc cgtctttcac ttcaaacagg ttttcacctg cgccttcaga
     7261 gatgtaaccg ttcacatcca gcgcgatacc ttcctgataa ccgtggcggc gcgcttcgct
     7321 acccaccagc agggaagaga ggtagttacc accggctttt gccgccgtcg ggatggtgtt
     7381 tggtgctgcg cggttccagg aggaaaccat cgcatcgatc ccctgctcca gcgcttctgc
     7441 gcccagatac gctccccacg ggaaagcagc gataatcacg tcggttgagt atcccgctgg
     7501 cgggtttact cccatgccaa catcaccgac gaagatcagc ggacggatat aggcgctggt
     7561 gagattattt ttgcggatca cgtcacgaca agcttccatc agctcatcaa tgctctgcga
     7621 aaccgggaag cgatagattt tggcggagtc atgcagacgc tgcatatgct cacgatggcg
     7681 gaatacaacc ggtcctttgt gcgagtcgta gcaacggatg ccttcaaaaa ccgaagtgcc
     7741 atagtgcagc gcgtgcgaca tcacatgcac cttcgcgtct tcccagcgaa ccatctcccc
     7801 attgaaccaa atgtaatcag ctttcttcgt ggtcattttt atattccttt tgcgctcagg
     7861 cgcggatttg ttgtgatgtg gttgtgctct ggcagatggc aacgtgtgcg acgtccacca
     7921 gtttatttaa ctgactaaac agtaagtcga ccgaccgtgg gctggcaacg gtcaattcga
     7981 tatttatatt ttgtgcatcg ctggcggcgg ccatattcat tgagcagacg tggaaaccac
     8041 gatgacgcac cacgcgtaaa acacgttcta aggtttctgg attgaagcga gccgatacat
     8101 tgacctgatg ttgcatcatg ataatttctc caacatttct gaattactgg cgccaggcgg
     8161 caccagcggc cagacgttct caagttcgtc gattgagaca tgaagcaggt atggcccatc
     8221 actgttcagc atggtgtcga gtgccgcttc aacctggtct ttacgggtga tgtgttggcc
     8281 agggatgccg aaggcgctgg ctaacatgag gaaatcgggg ttatcagtaa gggtggtttc
     8341 gctgtatcgt tcctgaaaaa acagttgctg ccattgtcga accatcccta accgttggtt
     8401 atcgagtaag acgattttca acggtaactg cttgcgtttt acggtgccca gctcttgcac
     8461 attcatcatg aaagagccgt caccggagat acagacaacg gtatcgttcg gtcgcgcgac
     8521 ttgtgcgcca accgccgccg gtaaaccaaa acccatggta cctaagccgc tggaggtgat
     8581 gaaattttcc gggcgagtgt gggcgatgtg ctgcgcagcc cacatctggt gctgccccac
     8641 atctgtggtc acgacgcaat ccgcaggttt acgatccgac agttgtttta acaacaacgg
     8701 cgcgtagata gcgtcaccgg gatggtcgta acgccaggaa tgttcatcac gcagctgcgc
     8761 gcagtattgc tgccagtcat tgatatttaa cggctgctgt aatgctggta acagagcatt
     8821 taaatcaccc tgtaatgcca catgtgcctg acgcagcttg ttcatttctg ccgggtcgat
     8881 atccatatgg ataacactgg cgtgtggtgc gaaagtattc agtttgccgg tcacccggtc
     8941 atcaaaacgt gcgcccacgg cgatcagcag gtcacactcc tgcaccgcga agtttgccgc
     9001 tttgatgccg tgcatcccca gcatgcccag atagtacgga taatctgctt ctactgcgcc
     9061 cagccctttc agcgtacagg tggcaggcat ttttgtggca gcgagaaatt cacgcaaagc
     9121 cggaactgcc tgcgccatac ccacgccacc gccaacgtac agcatcggtt tttgcgcttt
     9181 tgccagcatc tggcgcgctt gctcaacttc ggcatgtggg aaagtcactt cgttttcaac
     9241 ggtggtgaac cacggttcca ggtcaccgct ggctaactgg atatcttttg ggatatcgac
     9301 cagaaccgga ccaggacgac ctgagcaggc aacgtcgaat gcttcagcca tgatgcgcgg
     9361 caactcttcc agcgactgca ccagaaagct gtgcttggta caggctaacg acaatcctag
     9421 gacatccact tcctgaaatg cgtcagtgcc gataaacggt gcggacactt gaccggtgat
     9481 ggcaacaaca gggatggaat ctaacagtgc gtccgcaagc ccggttatca ggttggttgc
     9541 gcccggacca gacgtggcga tacatacgcc agttttgccg gtagcacggg cataaccgat
     9601 agccgccatt gccgcaccct gctcatgtcg gcatagcaag tgctccacgc cgccgtcata
     9661 caatgcatcg taaaccggca taattgcgcc acccggataa ccgaaaacgg tgttcacacc
     9721 ctgtgcccgc aacgcatgta ccacccactg tgcgccattc atagttagtt ccccgtcctg
     9781 aatcttgaga aacagaattt tgtgctgtta ttcattgtct gctcctcggt tatgttttta
     9841 aggtcaaaaa aaacccccgg acctttcggt gcgggggtct tagttcgtta aggcttgatc
     9901 tctaagcctt tcctcgtcca agtgcagccc cgcacggtgg gataataatc accaccacgc
     9961 taatcacgac taggctaatc actcgtagaa gggctgtcat tttgtctttt cttgcatctt
    10021 gttcgaagga atgcctaaag agttaccata gattttgcga atagcacaag atatttttgg
    10081 gcctattttt atgatagctt taataatttc aatagaagtt gttgtttttt aaaaagaaaa
    10141 tagtgagtga aaaaaattca atttcagtat aaatgttgtt cccttcctcg tagttcatcc
    10201 tttgtccgct gcatttttgc gagcatcatt ccaccgccag aacaactcaa cttcacttgc
    10261 acaaataaac aggaatcagc cactaaaatt cacgaattag cgggctacag cagcgatatc
    10321 ttatgcacat aatggtgact caaggagggc ttatgtcact gtcaattgtt catacccgcg
    10381 cagccctggg agtaaatgcg ccccctgatc actgttgagg tacatatcag taaaggtcta
    10441 cccggcttaa cgatggtggg cttaccagaa acaactgtaa aagaagctcg cgatcgcgtg
    10501 cgcagcgcca ttatcaatag cggatatgaa tatccggcga aaaaaatcac catcaacctt
    10561 gctccagccg atctgccaaa agaaggggga cgatatgatt tacctatcgc cattgcgttg
    10621 ctggcggcct cagaacagct tacagccaat aagttagatg aatatgaatt agtcggagaa
    10681 ctggctctta caggcgctct gcgtggcgtt cccggtgcaa tctccagtgc aactgaagct
    10741 attaagtcgg gcagaaaaat tatcgtcgcg aaagataacg aagatgaagt ggggctaatt
    10801 aacggtgaag gatgcctgat agccgatcat ctgcaagctg tctgtgcgtt tctggaaggt
    10861 aagcacgctc tcgaacgccc gaaaccaact gatgcagtat cccgggcgct acaacatgat
    10921 ctcagtgatg ttgtcggtca ggaacaagga aagcgaggac tggaaattac cgccgctggc
    10981 aggcacaacc ttttactgat tgggccgccg ggaacaggta aaacaatgct cgccagccgt
    11041 attaatggcc tgttgccaga tttaagcaat gaagaggcac tggagagcgc tgcgatatta
    11101 agtctggtaa atgctgaatc agtacaaaaa caatggcggc agcgcccgtt ccgctcacct
    11161 catcacagtg cgtcgttaac tgcgatggtg ggcggtggtg caattccagg gccaggtgaa
    11221 atttcgttgg cgcataacgg cgtgc
//
LOCUS       DAJTZL010000133        11000 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.133, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000133 DAJTZL010000000
VERSION     DAJTZL010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11000
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.133"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..277)
                     /locus_tag="OO544_003071"
     CDS             complement(<1..277)
                     /locus_tag="OO544_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6768600.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRS"
     gene            complement(444..1184)
                     /locus_tag="OO544_003072"
     CDS             complement(444..1184)
                     /locus_tag="OO544_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HCR6768601.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            complement(1277..1912)
                     /gene="argO"
                     /locus_tag="OO544_003073"
     CDS             complement(1277..1912)
                     /gene="argO"
                     /locus_tag="OO544_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HCR6768602.1"
                     /translation="MFSYYFQGIALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVAFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            complement(2051..2911)
                     /gene="mscS"
                     /locus_tag="OO544_003074"
     CDS             complement(2051..2911)
                     /gene="mscS"
                     /locus_tag="OO544_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HCR6768603.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            complement(3269..4348)
                     /gene="fbaA"
                     /locus_tag="OO544_003075"
     CDS             complement(3269..4348)
                     /gene="fbaA"
                     /locus_tag="OO544_003075"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HCR6768604.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            complement(4563..5726)
                     /gene="pgk"
                     /locus_tag="OO544_003076"
     CDS             complement(4563..5726)
                     /gene="pgk"
                     /locus_tag="OO544_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HCR6768605.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETASATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            complement(5776..6795)
                     /gene="epd"
                     /locus_tag="OO544_003077"
     CDS             complement(5776..6795)
                     /gene="epd"
                     /locus_tag="OO544_003077"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HCR6768606.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            7167..7598
                     /locus_tag="OO544_003078"
     CDS             7167..7598
                     /locus_tag="OO544_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR6768607.1"
                     /translation="MMTLPYPYSSPARFYALRLLPGQEVFSQLHAFAQQHQLHAAWIA
                     GCTGSLTDVALRYAGQEGTTLLNGTFEVISLNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGSLEELAFSRQPCALSGYDELHISPVK"
     gene            7621..8199
                     /locus_tag="OO544_003079"
     CDS             7621..8199
                     /locus_tag="OO544_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCR6768608.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVAGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            8200..8907
                     /locus_tag="OO544_003080"
     CDS             8200..8907
                     /locus_tag="OO544_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCR6768609.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVAWLMFSLGAGLWLVHGGWLTEWLSGTPRSPERWTHAITLWLRILAIVSTSQL
                     WMQYVPVQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRARGVPLDEG
                     WYQRLRAMPALIILLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADSTLQRVARY
                     TMILLMLTEFGAWIWLR"
     gene            8895..9572
                     /locus_tag="OO544_003081"
     CDS             8895..9572
                     /locus_tag="OO544_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HCR6768610.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPGEITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAETEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWALERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            9566..10243
                     /locus_tag="OO544_003082"
     CDS             9566..10243
                     /locus_tag="OO544_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HCR6768611.1"
                     /translation="MLTLNQISYRWPSAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGVIRNVN
                     PPDDIHP"
     gene            complement(10215..10928)
                     /locus_tag="OO544_003083"
     CDS             complement(10215..10928)
                     /locus_tag="OO544_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HCR6768612.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKPLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLVQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPATALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(10925..>11000)
                     /locus_tag="OO544_003084"
     CDS             complement(10925..>11000)
                     /locus_tag="OO544_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR6768613.1"
                     /translation="ARFSGMVKTALTLAVTTTLKELTP"
BASE COUNT         2626 a         2919 c         2741 g         2714 t
ORIGIN      
        1 gtgaacgacg ccctgagtta tggatttcat tatcaatcgc agaaagtcgt tgataaaccg
       61 ggaacagttt ttgatacagt tcctgaccgg ctttattgag gctgatatta ttatctttgc
      121 gggtaaatag cgtatagcca atttgttctt ccagcgcggc aatgctttta ccaaatggcg
      181 aagccgtcat atggattttt tctgccgccc gggcaatatt attggtttgc gctaataaaa
      241 tgaaattgcg catctttttc gaaataaaaa tgtccatccc ccctccccgc atccttgtcc
      301 gcttaaccgt ttcagtcagc ctatccttga tgaaaccgcg agcaaagata ggtgattacg
      361 tcatggtttt acagaaaatt acagaaaaag gaggcaatat cgggtaaagg cattagcccg
      421 acgaatacgt cgggctacaa gtattattgt gctgcaggtg ttttagcggg ttgttgatcc
      481 acaggttcta actggaagac cacatcgacc tgatcatcaa actgaatagc ggcctgctcg
      541 taagtttcct gggcagacac cggcgcggca tcggctttca tcatccgcac cattgggctg
      601 ggctgatagt tggaaacatg gtagcgcacg ctatataccg gccccagttt acgatgaaag
      661 ccgttcgcca gttcctgcgc ctgatgaatc gcgttatcaa tcgctgcctt acgcgctttg
      721 tctttatagg catccggctg cgccacgccc agcgacacag aacgaatttc gttaagaccc
      781 gccttcagcg cgccatccag caaggaattc agtttgtcta actgacggag cgtgacttcc
      841 accgttctca cagcgcggta gcctttcagg atacttttac catcctgata atcataatct
      901 ggctgggtgc gtaagttcgc tgagctgata tcttttttcg cgatctgatt gagttcaagg
      961 aaggaaatgt attgtgcgac gcgctcatct gcctgtttct tggctgtagc ggcatccttc
     1021 gcggccacgt taacttcaat cgcaagagtg gcaatgtctg gcaccgcatc cacacttgcc
     1081 gtaccggagg tgacaatatg cggtccatcc ggcaattcgt tagcctgcgc tgccatcccg
     1141 ctaataccca ttaatgccgc cagggcgata actttgaact tcacgttagt ctctccgtgt
     1201 taattgactt gcccaaaatt caggacctct ggaggcaagc ttagcgcctc tgttttattt
     1261 ttccatcaga tagcgcctaa ctgaacaagg cttgtgcatg agcaataccg tctctcgcca
     1321 gctgcaaggc tataaaccac atgacacatc ccactaccag attgataatg cgctgtgatt
     1381 ttgccgtgcg caggcgcggt gccagccagg ctgcgagaat agccagacca aagaaccaca
     1441 ggaaagaggc gctaattgtc ccgagcgcaa accaacgttt tggttcgacg tcaagctgcc
     1501 cgccaaggct gcccagtaca acaaaagtat ccaggtaaac atgcggattc agccaggtca
     1561 ctgccagcat ggtggcgata attttccatc tgccttgctt caggacttcg gcactagcta
     1621 actcaatatt actactcatc gctgttttaa aagcgccaaa accataccac agcaagaagg
     1681 ctacgccgcc ccaggtgacc agcgccagca accacggcga ctgcatcaat aacgcgctgc
     1741 caccaaaaat cccggcgcaa atcaggacca aatcgctgat agcacaaagt aaggcaatca
     1801 taatgtggta ctgacgacga atgccctgat tcatcacaaa agcattttgt ggaccgagcg
     1861 gcagaatcat agctgcccca agagcaatac cttgaaagta ataagaaaac acgttggata
     1921 ttccgaatta agtatctgat gcagagacta tatcgcggga ttgttattag cggaaattga
     1981 taatattaat tggcaatcag aataactaat aagagagagg ccagagagta ttatctggcc
     2041 tcagcgttga ttacgcagct ttgtcttctt tcacccgctt aaagttcaca tccatttgcg
     2101 ggtacgggaa gctgataccg gcggcatcaa attcacgttt aatacgctcc agcacatccc
     2161 agtacacgtt ttgcagatcg ccgctgttgc tccagacgcg gaccacgaaa ttaatcgacg
     2221 atgcaccaag ttcgttcaga cgcacagtca tttcgcgatc tttcaaaatg cgatcttcag
     2281 actggataat attggtcagg atctgcttaa cctgatcgat atcggaatca tacgccacgc
     2341 caataataaa ttcgttacgg cgaactggct cgcgagagaa gttaataata tttccggcaa
     2401 taattttacc gttcggaata acgataattt taccgtctgc agtacgcatg gtggtggaga
     2461 aaatctgcac actcagcaca gtaccggcta cgccgccgag atcaacatat tctccggcac
     2521 ggaacgggcg gaacatgaca agtaacacgc cagcggccag gttagaaagt gacccctgca
     2581 aagccagacc aacagctaag cctgcggcac cgagtacagc aatgactgac gctgtttgta
     2641 cacccacgcg tcccagggca gcgattagcg taaaggcgat aataccgtaa cggactaatg
     2701 cagaaagaaa atcagcaaca gtggcatcga ttttacggga gatcatcagg cgattcaccg
     2761 cgttggaaat catccgcgcg ataatcaaac caacgatgat gatcgcgagt gccgccacga
     2821 tgtttactgc ataacttagc agcagcgcct ggttagctac cagccagctt cccgcgccgt
     2881 ttatgctatc gacaacattc aaatcttcca ttcaatattc cttttcaact gactcaaaat
     2941 ggagaaatac actccgccgc cttatgacgg gcagtctgac aagggtaaac aataaagcat
     3001 aattttgcca aatagatcac agatttctca tctgaatcac cttgcttttg gcgaaaaaat
     3061 ccccgcagag cggggctcag acagatatca atgtgcgctt tgtcatgccg gatgcggcgt
     3121 gaacgcctta tccggcctac aaaaacctgc aaattcaata tattgcagga tacgtgtagg
     3181 cctgataagc gtagcgcatc aggcaatttt gcttttggcg aaaaaaagac ccgcagagcg
     3241 ggccttgaga taagcagaaa ggaatatctt acagaacgtc gatcgcgttc agttcctgga
     3301 atgctttttc cagacgagcg atcatcgaag tctgaccggc acgcagccat acgcgcggat
     3361 cgtagtattt cttgttcggc tgatcttcgc ctttcgggtt acccaactgc ccctgcagat
     3421 aagcttcgtt cgctttgtag tagttcagaa cgccttccca ggttgcccat tgggtatcgg
     3481 tatcgatgtt catttttact acgccgtagc ttacggagtc tttgatttcc tgagcagtag
     3541 aaccggaacc accgtggaat acgaagttca ggctgttgtg cggcaggttg tgtttcttgg
     3601 aaacatattc ctgagaatca cgcaggatgg tcggagtcag aaccacgtta cccggcttgt
     3661 aaacaccgtg tacgttaccg aaggacgcag cgatggtgaa acgcgggctg attttgctca
     3721 gttcggtgta tgcgtaatca acgtcttccg gctgggtgta cagtgcagaa gcgtccatgt
     3781 ggctgttgtc cacgccgtct tcttcaccac cggtgcaacc cagttcgatt tccagagtca
     3841 tgccgatttt ggacatgcgc tccaggtatt tagagcagat ttcgatgttc tcttgcagag
     3901 attcttcaga caggtcgatc atgtgagaag agaacagcgg cttaccggta gctgcgaagt
     3961 gtttttcacc cgcgtccagc agaccgtcga tccacggcag cagtttcttc gcgcagtggt
     4021 cagtgtgcag gataaccgga acaccataat gttcagccat ctggtgaacg tgatgcgcac
     4081 cagagatcgc gcccaggata gcagcaccct gcggaacgtc agatttcacg cctttaccag
     4141 cgataaagga agcaccaccg ttggagaact gaacgataac cggcgcttta actttagcag
     4201 cggtttccag tacggcgttg atggagtcag taccgacgca gtttactgct ggcagtgcga
     4261 agttgttttc ttttgctacc tggaaaactt tctgtacgtc atcaccagtg attacgccag
     4321 gttttacgaa atcaaaaatc ttagacatgt ctcttgtcct gtatcgtcgg gccttatact
     4381 catcatactt caagttgcat gtgctgcgtc tgcgttcgct caccccagtc acttacttat
     4441 gtaagctcct ggggattcac tctcttgtcg ccttcctgca actcgaatta tttagagtat
     4501 gaaaaaatgc gtgctctaaa agcgcgctga aacaagggca ggtttccctg ccctgtgatt
     4561 ttttacttct tagcgcgctc ttcgagcatc gctactgcag gcagtacttt accttccacg
     4621 aattcgagga atgcgccgcc gccagtggag atgtaggaga ttttgtcagc aatgccgaac
     4681 aggtcgattg ctgccagagt gtcgccgccg ccagcgatgg agaacgcttc gctgtctgcg
     4741 atagcgttag ccacgatttc agtacctttg cggaagttcg ggaattcgaa cacgccaacc
     4801 ggaccgttcc acagaatggt tttcgcattc ttcaggattt cagccagttc ctgagcggaa
     4861 gcatcaccga tatccaggat ctgctcgtca gctttcacat cgttaacaga tttcagggta
     4921 gccgatgcag tttcagagaa ctcggttgct acgcgaacat cagacggaac cgggatgttg
     4981 caggtggtca gcagacgttt agcttcgtca accaggtcag cttcgtacag ggatttaccc
     5041 acatcgtggc cttgtgccgc gataaaggtg ttagcgatac caccaccaac aatcagctgg
     5101 tcagcgattt tagacaggga gtccagaacg gtcagtttgg tagatacttt agaaccacca
     5161 acgatagcca ccatcgggcg agcaggttct ttcagtgctt tacccagcgc gtccagttca
     5221 gctgccaaca gcgggcctgc gcacgcaaca tcagcgaatt taccgatacc gtgagtagaa
     5281 gcctgcgcgc ggtgagcagt accgaatgcg tccattacga acacgtcaca cagtgcagcg
     5341 tattttttgg acagggtttc gtcgtctttc ttctcgcctt tgttgaagcg aacgttttcc
     5401 agaacaacca gttcaccttc agcaacgtca acgccgtcga ggtaatcttt aaccagacga
     5461 accgggttag acagtttgtc tttcaggtag ttaacaaccg gcagcagaga gaattcttcg
     5521 ttgtactcgc cttcggtagg acgacccagg tgggaagtta ccatcacttt cgcgccttgt
     5581 ttcagggcca gttcgatggt cggcagagaa gcacggatac gcgcgtcgct ggttactttc
     5641 ccgtctttta ctggtacgtt cagatccgca cggataaata cacgtttccc agcaagatcc
     5701 agatcggtca tcttaattac agacatggtg aatcctctcg ttgattctaa aagttttgca
     5761 gacgctgctt gcgtcttacc tgaaagcaac agtagccata gctaacgtcg tgtcgagcat
     5821 tcggttagca aagccccatt cgttatcgca ccagaccaac gttttgatca ggtgtgcgcc
     5881 actgacccgg gtttgggtgc catcgacaat ggcactgtgc ggatcgtggt taaaatctac
     5941 agagaccaac ggcaattccg tatagtcaac tataccatga aatgcacctt gtgctgcttt
     6001 ttgcagcaac aggttgactt cattggcttt tacaggtttc ttcaccgtca cgcttaaatc
     6061 gattgccgtc acatttatgg ttggcacacg taccgcaatc gcttcaaagc gatcgttaaa
     6121 ttgcggaaaa aatcgtgtga taccggcggc cagtttagta tcgaccggaa tgatcgactg
     6181 gctggctgcc cgggtgcgac gcaggtcagg atggtatgca tcaataacct gttgatcgtg
     6241 catggcggag tgaattgtgg tcacagtgcc ggactcaata ccgtacgcat catctaacaa
     6301 tttgatgacg ggaattatgc aattcgtggt acaggaagcg ttagaaacga tgcggtgttc
     6361 cgcacgaagt tgatcctgat tgacgccgta aacaacggtc gcgtcgagat cgttactgcc
     6421 aggatgtgaa aagagcactt ttttggcccc ggcggcaata tgcgcttcgc catgctcgcg
     6481 ggagccatat acgccggtgc agtcgaggac tacatcaacg ccaagttcac gccaggggag
     6541 cgattgcagt gaacgttcat gcaatacgcg gatggcgtca tcaccaacaa aaagttgatc
     6601 gcgttcctgt cgtacttccc atgcaaaacg gccatggctg gtgtcatatt tcaacaaatg
     6661 cgccatgccc gcagcatccg ccagttcgtt gattgccacc acggtaattt ccgcccggcg
     6721 tccggattca tacaaagcac gaaccacatt acgcccgatg cgaccgaagc catttatcgc
     6781 tacgcgtacg gtcatagatc tcctgcaagg ttttccctga gcaaatttgc cagacagagt
     6841 aatccagcaa atcgtccggg gaaaccttac ctgtcgcaaa ctgcgactga ttggttaatt
     6901 gtcgaacatt taatcgactg aaacgcttca gctaggataa gcgaaacgtg gaataaaagg
     6961 aatgattgtc cagtcaaaga aaacaattat ctgacttacg tcacgttttc agccccgccc
     7021 ctgtggaatt tattccactt tgcagaatca cgaataagcg tagcaaaatc ttttcgttgt
     7081 gccgtcacaa accctgactt atatcagaat atgactgatg cagcgtcgat attatccgca
     7141 atacgtccga gtttcggagt actccaatga tgacattgcc ctacccatat tcttcccccg
     7201 cacgctttta tgcactgcgc ctcctccccg ggcaggaagt tttttcccag ctacacgctt
     7261 ttgcacagca gcatcaactt catgccgcct ggattgcagg ctgtaccggt agcctgactg
     7321 acgtcgcctt gcgctatgcc gggcaagaag gcaccacgct ccttaacggt acattcgaag
     7381 tgatctcact aaacggcacg ctggaacaga gtggtgagca tcttcatctc tgcgtttccg
     7441 atccacacgg cacgatgctt ggcggtcata tgatgcccgg ctgtaccgtg cggaccacgc
     7501 ttgaactggt gatcggtagc cttgaggaac tggcgtttag ccgccaacct tgcgcgcttt
     7561 ctggttacga cgagttgcat atttcccccg ttaaataact actttcaaag aggttttctt
     7621 atggcgcgta gccacttttc cagtcaggca ctggtgttga tcgttatatc tatcgccatc
     7681 aatatgatcg gcgggcaact ggccagcatg gtcaaattac ctatatttct cgactccatc
     7741 ggcacactta ttagcgcggt actgcttggt ccggtgatcg gaatgctgac cggcttactg
     7801 accaatttac tctggggatt gctgaccgac ccgatcgccg ccgcatttgc acccgtcgcg
     7861 atggtcatcg gcctggtcgc cggttggctg gcgcgagcgg gttggtttcg caccctgcca
     7921 aaagtcgttg tcagcggcgt gattattacg cttgcggtaa cggtcgtcgc tgtaccgctg
     7981 cgtactgcgc tgttcggcgg tgtcaccggt agcggagccg atttgttcgt tgcctggatg
     8041 cactcgatgg ggcaaaacct ggtggagtcg gtggcgatta cagtgatagg cgcgaacctg
     8101 gtcgataaaa ttcttacagc ggtgattgtc tggcttttac tgcgccagct gccgattcgc
     8161 accacgcgcc attttccggc aatggctgcc gtgcgctaaa tgcatccatt tacctcatta
     8221 acactgtggg cattggcggc ctgcaccacg cttatcctgc ccgcgcagac cattttgccg
     8281 atctatagcg cggcgacctt tttttgtctt attgcgctaa aggccacgcg gcggcgggca
     8341 aaatatgtcg cctggctgat gttctcgctg ggagccgggc tgtggctggt acacggtggc
     8401 tggctaacag aatggctaag cggcacgccg cgttcgccgg agcgttggac gcatgctatt
     8461 acgctctggt tgcggattct ggcgattgtt tcaacctcac agttgtggat gcagtacgtc
     8521 cccgtgcaat gttttatccg cgctctgttt gcttctcgcc tgccgccagg cgtcgcttat
     8581 ctgtttgccg gaccgttatt ggtcgttgag cagttaaaac ggcaattagc gattattcat
     8641 gaagcgcagc gtgcccgcgg cgtgccgctg gatgagggct ggtatcagcg tttacgtgcg
     8701 atgcccgcgt taattattct gctaacacac aatgcgctta acgatctggc tgtccgtggt
     8761 gcggcgctgg atatgcgggc atttcggatc cacaaccgac gaacaacgtt gtgggcacca
     8821 gccgatagca ccctacaacg cgtagcgcgt tacaccatga tcttgctgat gctaacagaa
     8881 ttcggagcgt ggatatggtt acgttagaac agttccgtta ttgcccgaca cactcaacgc
     8941 atcctccgtt ctgttatgac tttcactatg tcaaaccggg gatggtcgcc atctttggtg
     9001 acaacggcag tggcaaaagt acgctggcac agctgatggc gggctggtat ccggactttc
     9061 tgccaggaga gataacaggc acggggacgc tcctcggtac gcccattggg catctcccgc
     9121 ttaatgaaca atccgccacc atccagctcg tccagcaatc gccctatttg cagctctccg
     9181 gctgtacctt tagcgtggaa gaagaagttg cctttgggcc agaaaatctg tgtcttgctg
     9241 aaacagagat tatggcgcgg attgatgccg ccctgactct gaccgagtgc cagccgttgc
     9301 gccatcgtca cccggcaaca ctctccggtg gagagacaca acgcgtggtc atcgcctgcg
     9361 ccatcgcgat gcagccaaag ctcttaattc ttgatgaagc ctttagtcgc ctgacgccgc
     9421 aggccagcga aatgctgttg cagcgcttac aacactgggc gcttgaacgt ggttcgctga
     9481 ttattctttt tgaacgtcat cgcacacctt tcctgaacta ctgccagcaa gcgtggcaat
     9541 tgcaaaacgg agcgctccag ccactatgtt aacgttaaat cagatatctt atcgctggcc
     9601 tagcgcagca acggattgtc tttgcgacat ttctctgcaa ctcaaacaag gtgaatggtt
     9661 ggcgctgacc ggcgacaatg gcgcgggaaa atcgacgctg ttacgagtaa tggcaggtct
     9721 tcttacgccc acagccggta cggttatgct gcaacaacag gcgatgaaaa acctgaaaaa
     9781 ccgccagcgc gcggcaaagg ttggcgtact gtttcaggaa gcggaaaacc aactttttca
     9841 cagcaccgtc gctgacgaga tcgcatttgg tttgaaactg caaaaatgcc cggcggatga
     9901 aatcactcaa cgtacccatg ctgccctgca atgttgtcag ttagcagaca ctgccaactc
     9961 gcacccgttg gatctgcata gtgcgcagcg acggatggtc gccgtcgcct gcctggaagc
    10021 actctctccg ccactattac tgctggacga gccgagtcgg gattttgacg aaaattggtt
    10081 gagcgtgttt gaaagctggc tggagaagtg ccgccagcga ggcacgagcg tggtagcaat
    10141 cagtcatgac gccgcattta cccggcgtca tttctctcgt gtggtgcgac tggaagatgg
    10201 tgtaatcagg aacgttaatc caccagacga tattcacccg tagcggtcat cattaaggtc
    10261 aaatttgcag gcagactctc ttccagcacc cggcgaacat tcggcccgtc ggtacggtca
    10321 taaaaggctt ctgcatctgc cagcgatacc cctcccgcca gcttgcgccc caccagacgc
    10381 tcccgcaacg cagtagctgg cgcgttgata aagatcgaga aatcacaaaa ttgcgcaagc
    10441 tggcaccact tctcgtcgtc cagcaacaac cagtttcctt cgacgatgac gagtggtgcg
    10501 gtaacgtgca acgcatcttc aacaggatca tgcttttgtc gatcgtactg cggccacgta
    10561 caatcccctt ccaccacctg gcgcagattt tccgccagtt tcgcaacgtc aaatgtctct
    10621 ggtgcgcctt tgaaggggcg caattgatgc gcgtccagcc agctattgta atggtgaaaa
    10681 ccatccatcg ggagcgtctg aatagcgggc agctccgggt cttgttgcac gagatattcc
    10741 cagaaggtgg ttagggtaga tttacccgtc cccggcggag cgcaaagaaa aaccaccgta
    10801 cgccgctgcg gatttaccgt ctgtaaggcc gccagcatac gcagcaacgg tttatgcacg
    10861 cgttctatct cttcatcatg gtactgcgct tgcacgttca gcccattcac cgttaattca
    10921 attttcacgg cgttaattcc tttaaggtgg tcgttactgc cagcgtcagc gccgttttga
    10981 ccattccgct aaaacgcgcc
//
LOCUS       DAJTZL010000134        10990 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.134, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000134 DAJTZL010000000
VERSION     DAJTZL010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10990
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.134"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..1224
                     /gene="narU"
                     /locus_tag="OO544_003085"
     CDS             <1..1224
                     /gene="narU"
                     /locus_tag="OO544_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HCR6768614.1"
                     /translation="VTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGG
                     RRWTVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFP
                     KAKQGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAW
                     IWVPLLAIATIAAWSGMNDIASSRASIDDQLPVLQRLHLWLLSLLYLATFGSFIGFSA
                     GFAMLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSA
                     LLFLTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSD
                     EQAQKEAITETAAALGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCV
                     LLTWLVYGRRKFSQK"
     gene            1306..5046
                     /gene="narZ"
                     /locus_tag="OO544_003086"
     CDS             1306..5046
                     /gene="narZ"
                     /locus_tag="OO544_003086"
                     /EC_number="1.7.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
                     /protein_id="HCR6768615.1"
                     /translation="MSKLLDRFRYFKQKGETFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDQPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKCLSYKQVRGRGGFIR
                     SNWQELNQLIAAANVWTIKIYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMIRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGKETELSLTLLGQHDAVAGVAFPYFGGIENPHFRSVKHNPV
                     LVRQLPVKNLTLVDGNTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKVS
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLGIKAEADKAGLSPTEF
                     TAQALKSGDLRMACEQPDSGSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     EELGASDGIKPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDVVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLIPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRVCPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            5043..6587
                     /gene="narH"
                     /locus_tag="OO544_003087"
     CDS             5043..6587
                     /gene="narH"
                     /locus_tag="OO544_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HCR6768616.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
                     EAMQKEMYGQFKNTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVIEAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSVAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            6587..7282
                     /gene="narW"
                     /locus_tag="OO544_003088"
     CDS             6587..7282
                     /gene="narW"
                     /locus_tag="OO544_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HCR6768617.1"
                     /translation="MQILKVIGLLMEYPDELLWECKEDALALIRRDAPMLTDFTHNLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLDCRE
                     LPDYLPLYLEYLSVLPDDQAKEGLLNVAPILALLGGRLKQREAPWYALFDALLQLAGS
                     ILSSDSVTKQVNSEERDDTRQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDV
                     APQYVDISAGGGK"
     gene            7279..7959
                     /gene="narI"
                     /locus_tag="OO544_003089"
     CDS             7279..7959
                     /gene="narI"
                     /locus_tag="OO544_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HCR6768618.1"
                     /translation="MIQYLNVFFYDIYPYICATVFFLGSWLRYDYGQYTWRASSSQML
                     DKRGMVIWSNLFHIGILGIFFGHLFGMLTPHWMYAWFLPVAAKQLMAMVLGGICGVLT
                     LIGGAGLLWRSLTNQRVRATSTTPDIIIMSILLIQCLLGLSTIPFSAQYPDGSEMMKL
                     VGWAQSIVTFRGGSSEMLNGVAFVFRLHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            8038..8931
                     /locus_tag="OO544_003090"
     CDS             8038..8931
                     /locus_tag="OO544_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HCR6768619.1"
                     /translation="MKPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRAKVLGLGNCTIWQTSLAGK
                     HRVTIEKHNDDYRISLEQGTPGFELPLEGEVRAAIINALHLTEDDILPGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLNALTAISKQIGCNGFFPFQIRPGKNETDGRMFSPAIG
                     IVEDPVTGNANGPMGAWLVHHNVLPHDGNVLCVKGHQGRALGRDGMIEVTVTIRDNQP
                     EKVTISGTAVILFHAEWAIEI"
     gene            complement(9027..9872)
                     /gene="nhoA"
                     /locus_tag="OO544_003091"
     CDS             complement(9027..9872)
                     /gene="nhoA"
                     /locus_tag="OO544_003091"
                     /EC_number="2.3.1.118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
                     /protein_id="HCR6768620.1"
                     /translation="MTPILNHYFARINWSGAAAVNIDTLRALHLKHNCTIPFENLDVL
                     LPREIQLDDQSLEEKLVIARRGGYCFEQNGVFERVLRELGFNVRSLLGRVVLSNPPAL
                     PPRTHRLLLVELEGEKWIADVGFGGQTLTAPIRLVSDLVQTTPHGEYRLLQEGDDWVL
                     QFNHHQHWQPMYRFDLCEQQQSDYVMGNFWSAHWPQSHFRHHLLMCRHLPDGGKLTLT
                     NFHFTHYENGHAVEQRNLPDVASLYAVMQEQFGLGVDDAKHGFTVDELALVMAAFDTH
                     PEAGK"
     gene            10045..10614
                     /locus_tag="OO544_003092"
     CDS             10045..10614
                     /locus_tag="OO544_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HCR6768621.1"
                     /translation="MSRFIPIELHHASRLLNHGPTVLITSFDEQSQRRNIMAAAWSMP
                     VEFEPPRVAIVVDKSTWTRELIERNGKFGIVIPGVAATNWTWAVGSVSVREEDKFNCY
                     GIPVVKGPVLGLPLVEEKCLAWMECRLLPATSAQQKYDTLFGEVVSAAADARVFVEGR
                     WQFDDDKLNTLHHLGAGTFVTSGKRVTAG"
     gene            complement(10618..10845)
                     /gene="pptA"
                     /locus_tag="OO544_003093"
     CDS             complement(10618..10845)
                     /gene="pptA"
                     /locus_tag="OO544_003093"
                     /EC_number="5.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase PptA"
                     /protein_id="HCR6768622.1"
                     /translation="MPHIDIKCFPRELDEQQKAALAADITDVIIRHLNSKDSSISIAL
                     QQIQPESWQAIWDAEIEPQMEALIKKPGYSM"
BASE COUNT         2555 a         2866 c         2956 g         2613 t
ORIGIN      
        1 gttaccgtta atctcaataa aatcggtttt aattttacta ccgatcaact ctttttatta
       61 accgcattac cctccgtttc tggcgcatta ttgcgtgttc cctactcctt tatggtgcct
      121 atattcggtg gacgccgatg gacggttttt agtactgcaa tcctgattat tccttgcgtc
      181 tggctcggaa ttgccgtgca aaatccgaat actccttttg ggatatttat cgttatcgct
      241 ttgctatgcg gttttgcagg tgcaaacttt gcttcgagca tgggcaatat cagtttcttc
      301 tttccaaaag ccaaacaagg gagcgctctt gggattaatg gcggattagg aaacttaggt
      361 gtaagtgtaa tgcagctggt tgcaccgctg gtcatttttg tacctgtatt tgcctttctc
      421 ggcgtcaatg gcgtaccgca ggccgacggt tcggtgatgt cgctggcgaa tgccgcatgg
      481 atttgggtac cgctactggc gattgccacg atcgccgcct ggtcagggat gaatgatatc
      541 gccagttcac gcgcctcaat tgacgaccag ctccctgtct tacaacgcct gcatctctgg
      601 ctgctgagcc tgctttacct tgccaccttc ggttcgttta tcggtttttc tgcgggtttt
      661 gccatgctgg caaaaaccca gttcccggat gtgaatattc tgcgcctggc gttctttggc
      721 ccatttatcg gtgccatcgc gcggtcggtt ggtggtgcta tttccgataa attcggcggc
      781 gtgcgggtga cgttgatcaa ctttattttt atggcgattt tcagtgccct gctgttcctt
      841 accttaccgg gcacaggctc cggtaatttc atcgcctttt acgccgtatt tatggggctg
      901 tttctgactg cgggtctggg aagtggttct actttccaga tgatcgccgt catctttcgc
      961 cagataacca tttatcgggt gaagatgaaa ggcggtagtg atgagcaagc tcagaaagaa
     1021 gccatcaccg aaacggcggc ggctctgggc tttatctcag ccattggcgc agtgggcggc
     1081 ttttttattc cgcaggcgtt tggcatgtcg ctcaatatga ccggctctcc ggtcggcgcg
     1141 atgaaagtgt ttttaatctt ctacatcgtt tgtgtgctgc tgacctggct ggtttatggt
     1201 cggcggaagt tcagccaaaa ataagagatc gatgcctgat gtacaaccgt gtcgcatccg
     1261 acaaggcatc agatcccaat tcattcctgg agcaggagtc atgtcatgag taaacttttg
     1321 gatcgctttc gctacttcaa acaaaagggc gaaacctttg ccgatggtca cggacaggtg
     1381 atgcatagca accgcgactg ggaggacagc tatcgccagc gttggcagtt cgacaaaatt
     1441 gtgcgttcca cccacggtgt taactgtaca ggctcctgta gctggaaaat ctacgttaaa
     1501 aatggtctgg tgacctggga aatccaacag accgactacc cgcgcactcg ccctgaccag
     1561 cccaatcatg aacctcgcgg ctgcccgcgt ggcgcaagtt actcctggta tctctacagc
     1621 gctaaccgcc tgaaataccc gctcattcgt aaacgactga ttgaactgtg gcgtgaagct
     1681 ctcaagcaac acagcgatcc ggtactggcg tgggcatcga ttatgaacga tccgcaaaag
     1741 tgcttaagct acaaacaagt gcgtgggcgc ggcgggttta tccgctccaa ctggcaggaa
     1801 cttaaccaac tgattgccgc cgctaacgtc tggaccatca aaatctatgg tccggatcgc
     1861 gttgccggtt tctcgccgat cccggcgatg tcgatggtgt catacgctgc cggaacgcgt
     1921 tatctgtcgc tgttaggcgg cacctgttta agcttctacg actggtattg cgacctgccg
     1981 cccgcctcgc cgatgacctg gggcgaacaa accgacgtgc cggaatctgc cgactggtat
     2041 aactccagtt acatcatcgc ctgggggtct aacgtaccgc agacacgtac gccggacgcc
     2101 cacttcttta ccgaagtacg ttacaaaggc accaaaacta tcgccattac ccctgactac
     2161 tctgaagtgg ccaaattgtg cgaccagtgg ctggcaccga aacaaggcac tgatagcgcc
     2221 ctggcgatgg caatgggcca tgtgatttta aaagagtttc atctcgataa tcccagcgac
     2281 tactttatca actactgccg ccgctatagc gacatgccga tgctggtaat gctggaacct
     2341 cgcgacgatg gtagctacgt acccgggcgc atgatccgcg cgtctgacct ggtagatgga
     2401 ctaggcgaaa gcaataatcc gcagtggaaa accgtggcgg ttaataccgc aggtgaattg
     2461 gtagtgccga atggttcgat tggtttccgc tggggagaaa aaggcaaatg gaatctggaa
     2521 tccattgccg ccggtaagga aaccgaattg tcgttaaccc tgctcggtca acatgacgct
     2581 gttgcaggcg tggccttccc ctactttggc ggcattgaaa atccgcattt tcgcagcgta
     2641 aaacacaatc cggtgctggt gcgccaattg cccgttaaaa acctgacgtt agtcgatggc
     2701 aacacctgtc cggtggtcag cgtttatgat ttggtgctgg cgaattacgg cctcgatcgc
     2761 gggctggaag atgaaaacag cgcgaaagat tacgctgaaa tcaaaccgta caccccagcc
     2821 tggggtgagc aaattaccgg cgtaccgcgc cagtatattg aaaccatcgc ccgcgaattt
     2881 gccgataccg cccataaaac gcatggacgc tcaatgatta tcctcggcgc tggcgtcaac
     2941 cactggtatc acatggatat gaactaccgt gggatgatca atatgctgat cttctgcggt
     3001 tgtgtcgggc aaagcggcgg cggctgggcg cactatgtcg gtcaggaaaa actgcgccca
     3061 caaaccggct ggttgccgct ggcctttgcg ctcgactgga accgcccacc gcgccagatg
     3121 aacagtacct cgtttttcta caatcattcc agccaatggc gctatgaaaa agtctctgct
     3181 caggagttac tttcaccgct tgccgatgcc agtaagtaca gcggtcatct gattgatttc
     3241 aacgttcgcg ccgaacgcat gggctggcta ccttctgcgc cgcagctggg gcgtaacccg
     3301 ctcgggatta aagctgaagc cgacaaggcc ggattatccc ccacagaatt taccgcccag
     3361 gcgctgaaat cgggcgattt acgtatggcc tgcgaacaac cagatagcgg cagcaatcat
     3421 ccgcgtaatt tgtttgtctg gcgctctaac ctgcttggct cctccggcaa aggccacgag
     3481 tatatgcaga agtatctgct ggggaccgaa agcgggattc agggcgagga actcggtgcc
     3541 agcgacggga tcaaaccgga agaagtcgag tggcaaactg cggcgattga aggcaagctc
     3601 gacctgctgg tgacgctcga cttccgcatg tccagtacct gcctgttctc cgatatcgtt
     3661 ctgcccaccg ccacctggta cgaaaaagac gatatgaaca cctcggatat gcatccgttt
     3721 attcatccgc tttctgcggc ggtcgatccg gcgtgggaat cacgcagcga ctgggaaatc
     3781 tacaaaggta tcgccaaagc attttcgcaa gtgtgcgtgg gtcatcttgg caaagaaacc
     3841 gacgtggtat tacaacccct actgcatgac tctccggcag agctctcaca gccgtgtgaa
     3901 gtgctcgact ggcgcaaagg cgaatgcgat ctgattccag gcaaaaccgc gccgaatatt
     3961 gtggcggtgg agcgcgacta ccctgctacg tatgaacgct ttacctcgct cgggccattg
     4021 atggacaaac ttggcaatgg tggtaaaggg atttcgtgga atacccagga tgaaatcgat
     4081 ttcctcggca aactcaatta caccaagcgt gatggcccgg cacaggggcg tccgctgatt
     4141 gacaccgcca ttgacgcttc agaagtgatt ctggcactgg caccggaaac caacggtcat
     4201 gttgcagtca aagcgtggca ggcgctgggc gagatcaccg ggcgcgaaca tacccatctg
     4261 gcgctgcaca aagaggacga gaagattcgc tttcgcgata ttcaggcgca gccgcgtaaa
     4321 attatctcca gccccacatg gtccggtctg gaaagcgatc acgtctccta taatgcggga
     4381 tacaccaacg ttcatgagtt aattccgtgg cgcacgctgt cgggacgcca gcagctctat
     4441 caggatcatc cgtggatgcg tgcttttggt gaaagcctgg tggcttatcg cccgcctatc
     4501 gacacccgta gcgtcagtga gatgcgccag atcccgccaa acggcttccc ggaaaaagca
     4561 cttaacttcc tgacgccgca ccagaaatgg ggcattcact caacctacag tgaaaacctg
     4621 ctaatgctga cgctctctcg cggtggaccg attgtctgga tcagcgaaac cgatgcccga
     4681 gaactgacca ttgtcgataa cgactgggtg gaagtcttta acgccaacgg cgcgctgacg
     4741 gctcgtgcgg tggtcagcca acgtgtaccg ccgggtatga ccatgatgta tcacgcccag
     4801 gaacgcatta tgaatattcc tggttcggaa gtaactggca tgcgcggcgg cattcataac
     4861 tcggtcaccc gtgtttgccc gaaaccgacg catatgattg gcggttacgc gcagctggcc
     4921 tggggcttta actactacgg caccgtcggc tcgaaccgcg acgaattcat catgatccgc
     4981 aagatgaaga acgttaactg gctggatgat gaaggtcgcg atcaggtaca ggaggcgaaa
     5041 aaatgaaaat acgttcgcaa gtcggcatgg tgcttaacct cgacaaatgt atcggttgcc
     5101 atacctgttc ggtgacctgt aaaaacgtct ggaccggacg cgaaggcatg gagtacgcat
     5161 ggtttaacaa cgtcgaaacc aaaccgggca ttggttatcc gaaaaactgg gaagatcagg
     5221 aagagtggca aggcggctgg gtccgcgatg tgaatggcaa gatacgcccg cgtctgggca
     5281 gcaagatggg cgtaataacc aaaatcttcg ccaacccggt ggtgccgcag attgatgatt
     5341 actacgaacc tttcaccttc gactacgaac atttgcatag cgcaccggaa ggcaaacata
     5401 tccctactgc tcgcccgcgt tcgctgattg acggtaagcg gatggacaaa gtgatctggg
     5461 ggccaaactg ggaagaactg ctgggcggcg agtttgaaaa acgtgcccgc gaccgcaact
     5521 tcgaggccat gcaaaaggag atgtacgggc agtttaaaaa caccttcatg atgtacctgc
     5581 cgcgcctgtg cgaacactgc ctcaatccca gttgcgtggc gacctgccca agcggcgcta
     5641 tctacaaacg cgaagaagac ggcattgtgc tgattgatca ggataaatgc cgtggctggc
     5701 gtttgtgcat tagcggttgt ccgtacaaaa aaatctactt caactggaaa agcggcaagt
     5761 cagaaaaatg tatcttctgt tatccgcgaa ttgagtcagg ccaacccacc gtgtgctcag
     5821 aaacctgcgt gggtcgcatc cggtatctgg gcgtgctgct ttacgacgcc gaccgcattg
     5881 aggaagcggc gagcaccgag cgcgaagttg acctctatga acgtcagtgc gaagtgttcc
     5941 tcgatccaca cgatccctca gtgatcgagg aagccctgaa acaaggtatt ccacaaaacg
     6001 tgattgaagc tgcccagcgt tcgcctgtct acaaaatggc gatggactgg aaactggcgc
     6061 taccgctgca ccctgaatat cgcaccctgc caatggtctg gtacgttcct ccgctgtcgc
     6121 cgattcagtc ctacgcagat gcgggcggtt tgccgaaaag cgaaggcgtg ctgcccgcca
     6181 tcgaaagcct gcgtattccg gtgcaatatc tcgccaatat gttgagtgcc ggcgataccg
     6241 gtccggtact gcgggcgctg aaacggatga tggcgatgcg ccactatatg cgttcacaaa
     6301 ccgtggaagg cgttactgat actcgtgcca tcgacgaagt aggcctgagc gtcgcccagg
     6361 tcgaagaaat gtatcgctat ctcgccattg ccaactatga agatcgtttt gttatcccga
     6421 ccagccatcg ggaaatggcg ggcgatgcct tcgcagaacg caacggctgc ggttttacct
     6481 ttggcgacgg ttgccacggt tcggacagta aattcaacct gttcaacagt agccgtatcg
     6541 atgccatcaa catcaccgaa gtgcgcgaca aagcggaggg cgaataatgc agatcctcaa
     6601 agtgatcggc ctgttgatgg agtatccgga tgagctgttg tgggaatgca aggaggacgc
     6661 gctggcgttg atccgccgcg acgcgccgat gcttacggat ttcacccaca acctgcttaa
     6721 cgcgccgctg ctggataaac aggccgaatg gtgcgaagtg tttgaccgcg ggcgcaccac
     6781 gtcgctgctg ctgttcgaac atgttcatgc cgagtcccgc gatcgcggcc aggcaatggt
     6841 ggacctgctg gcggagtatg aaaaggtcgg cctgcagctg gattgtcggg aactgcccga
     6901 ttatctaccg ctgtatctgg agtatttaag cgtactgcct gacgatcagg cgaaagaagg
     6961 attgctcaat gttgcgccga tcctcgccct gcttggcggt cgcttaaaac aacgcgaggc
     7021 accgtggtac gcgttgtttg atgctctgct gcaactggcg ggaagtatac tttcaagtga
     7081 cagtgtcaca aaacaagtaa acagcgaaga gcgcgatgac acccgccagg cgcttgatgc
     7141 agtgtgggaa gaggaacagg tgaagtttat tgaagataac gccacggcgt gtgacagctc
     7201 gccgcttaat caatatcagc gacgctttag ccaggatgtc gcgccgcagt atgtcgacat
     7261 cagtgcggga ggtgggaaat gattcagtat ctgaacgtct ttttttacga tatctacccg
     7321 tacatttgtg cgacggtgtt tttcctcggc agctggctgc gctacgacta cgggcagtac
     7381 acctggcgcg cctcctcaag tcaaatgctc gataaacgcg ggatggtgat atggtcgaat
     7441 ctgttccata tcggcatttt ggggattttc ttcgggcact tgttcggcat gttaacgccg
     7501 cactggatgt acgcgtggtt tttgccagtg gcagcgaaac aactcatggc gatggtgctc
     7561 ggcggtattt gcggtgtgtt gacgttgatt ggcggcgcag gactgctgtg gcgcagtctg
     7621 acaaatcagc gcgtgcgtgc cacttccact acgccggata tcatcatcat gagcattttg
     7681 ctgatccagt gtctgttggg gctaagtacc ataccgtttt cagcacagta tcctgatggt
     7741 agcgaaatga tgaagctggt gggttgggcg caaagcattg tcactttccg tggtggatcg
     7801 tcagagatgc tcaacggcgt agcgttcgtc ttccgcctgc atctggtgtt gggaatgacc
     7861 atcttcctgc tcttcccgtt cacccgactg gtgcatgtgt ggagcgcgcc gtttgagtac
     7921 tttactcgtc gatatcaaat tgtgcgttcg cggcgataat ttcaccctgg ccggatagcc
     7981 cttcgctctc cggccacttg caggctatga tagttctcca gtcacaggag aacgttaatg
     8041 aaaccgcagg tgtaccacgt cgatgctttt acctctcaac catttcgcgg caattctgcc
     8101 ggtgttgttt tccccgccga taatcttagc gaagcacaaa tgcagcttat cgcccgcgag
     8161 ttaggccatt cggaaactgc ttttctgctg cacagcgacg acagcgatgt gcgcatccgc
     8221 tactttacgc caacggttga agtgccgatt tgcggtcacg cgacagtagc tgcgcactat
     8281 gtacgtgcga aggtgttagg tttaggaaat tgcacgatct ggcaaacatc gctggcagga
     8341 aaacatcgcg tgactatcga aaagcacaac gatgattatc gtatttcgct ggaacaaggt
     8401 acgccgggct ttgaactgcc gctggagggt gaagtacgcg cggcgattat caacgcattg
     8461 catcttactg aagacgatat tctgccgggt ttgccgatcc aggtcgcgac gaccgggcat
     8521 tctaaagtga tgatcccatt gaagccggaa gtggatatcg acgctctttc gcctgacctt
     8581 aatgcgctga ccgctatcag taaacagatt ggctgcaatg gtttcttccc gttccagatc
     8641 cgtccaggca aaaacgaaac cgatggtcgt atgttctcgc ctgcgattgg tattgtggaa
     8701 gatccggtga ctggcaatgc caatggcccg atgggtgcat ggttggtaca tcacaacgta
     8761 ttgccccacg atggcaacgt gttgtgtgtt aaaggccatc aggggcgcgc attggggcgt
     8821 gacggcatga ttgaagtgac ggtgacaatt cgtgataacc aaccggaaaa agtcaccatt
     8881 tcaggcacag cggtgatttt gtttcatgcc gaatgggcaa ttgaaatctg aaatcgttgc
     8941 ctgatgcgct tcgcctgtca ggtctacaac cgggctaatg tatgccggat aaggcgttta
     9001 cgccgcatcc ggcaacctga cataaattat tttcccgcct ccggatgcgt atcaaacgcc
     9061 gccatcacca gcgctaactc atccacagta aagccatgtt tcgcatcatc cacgcccaga
     9121 ccaaactgtt cctgcatcac ggcatataac gacgccacat ccggtagatt tcgctgctcc
     9181 accgcgtgcc cattttcata atgggtaaaa tgaaaattgg tcagtgtcag cttgccgccg
     9241 tccggcaaat ggcggcacat cagcaaatga tggcgaaaat gcgactgcgg ccagtgcgcc
     9301 gaccagaaat tgcccatcac ataatcgctt tgttgctgct cgcagagatc gaagcggtac
     9361 atcggctgcc aatgctgatg atgattaaac tgcaacaccc aatcatcacc ctcctgcaac
     9421 aaccgatact ctccgtgtgg cgtggtctgc acgagatcgg aaactaaacg aatcggcgcg
     9481 gttagcgtct gcccaccgaa accgacatca gcaatccatt tttccccttc cagttccacc
     9541 aacagcaaac gatgggtgcg cggcggtaat gctggcggat ttgataacac tacgcgccct
     9601 aacaagctgc gaacgttaaa ccccagctcg cgtaacaccc gctcaaacac gccattctgc
     9661 tcaaaacagt aaccgccacg acgggctatc accagtttct cttccagcga ttgatcatca
     9721 agctgtattt ccctcggcag caaaacgtca aggttttcaa acggaatggt gcaattgtgt
     9781 ttcaggtgca atgcacgaag cgtatcaata ttgaccgcag cagctcccga ccagttaata
     9841 cgggcaaaat agtgattcag aatgggcgtc atcaggcgtt tcccttagtg gttttctcaa
     9901 cttataacct ggaatagacg ccgcaccggc tattttgtgc gatcgcggac atgcatcacg
     9961 tttgcaaatc gccaatcccg ttaatttcgt tacttattgc gttacggact acgcttaaaa
    10021 aaaccttctt cgcgaggcga taccatgagc cgattcatcc ccatagaatt acaccatgcc
    10081 agccgtctgt taaatcatgg cccaactgtc ttgatcacca gtttcgatga gcaatcccag
    10141 cggcgtaaca ttatggctgc agcctggtca atgccggtgg agtttgaacc tccacgcgtg
    10201 gcaattgtgg tagataaatc gacatggacc agggagttga ttgaacgtaa cggtaagttt
    10261 ggcatcgtta tcccgggcgt tgcagcaact aactggacgt gggcggtggg aagtgtgtcg
    10321 gtgcgtgaag aagataaatt taattgctat ggcattccgg tggtgaaagg tccggtgctt
    10381 ggtttgccac ttgttgaaga gaaatgcctg gcgtggatgg agtgccgcct gctacctgcg
    10441 acttctgcgc aacaaaaata cgacacgctg tttggcgagg tggtatcagc agcggcagac
    10501 gcacgggtat ttgtcgaagg ccgctggcag tttgatgatg ataagctcaa tacgttgcat
    10561 catttaggtg ctggcacgtt tgttactagc ggcaagcgtg ttacggcagg ttaagcatta
    10621 catgctgtaa ccgggtttct ttatcaaagc ctccatttgg ggctcgattt cggcatccca
    10681 gatagcttgc caagattctg gttgaatctg ctgtagagca atgcttatcg agctatcttt
    10741 actgttcaga tggcgaataa taacgtcggt aatatctgca gcaagtgctg ctttttgttg
    10801 ttcgtccagt tcacgcggaa agcatttaat gtcgatgtgc ggcatgatta cgctcctttt
    10861 gaaagatgtc agtaaaatta acacacattg tgaatgtcat gtgtccggta aagcgtttat
    10921 acgacaataa tgcccagggc aagattacga aagcccgctc cccgtaagga ctgacgccag
    10981 gtagtttctg
//
LOCUS       DAJTZL010000135        10943 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.135, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000135 DAJTZL010000000
VERSION     DAJTZL010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10943
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.135"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..610
                     /gene="ttcA"
                     /locus_tag="OO544_003094"
     CDS             <1..610
                     /gene="ttcA"
                     /locus_tag="OO544_003094"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HCR6768623.1"
                     /translation="IVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDD
                     ILQTLFLNMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCN
                     LCGSQPNLQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGS
                     EVVNGGDLAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(739..2112)
                     /gene="dbpA"
                     /locus_tag="OO544_003095"
     CDS             complement(739..2112)
                     /gene="dbpA"
                     /locus_tag="OO544_003095"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HCR6768624.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAVISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVPLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     SRVRLLK"
     gene            complement(2590..3573)
                     /gene="zntB"
                     /locus_tag="OO544_003096"
     CDS             complement(2590..3573)
                     /gene="zntB"
                     /locus_tag="OO544_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HCR6768625.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            3828..5060
                     /gene="dgcM"
                     /locus_tag="OO544_003097"
     CDS             3828..5060
                     /gene="dgcM"
                     /locus_tag="OO544_003097"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HCR6768626.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRKEEETALNCRLVLRKLTEAEPVI
                     IFEGIEASATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFIHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
     gene            complement(5081..5644)
                     /gene="smrA"
                     /locus_tag="OO544_003098"
     CDS             complement(5081..5644)
                     /gene="smrA"
                     /locus_tag="OO544_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HCR6768627.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMM
                     FSFIQQAMADGLRNVLIIHGKGRDDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(5974..6882)
                     /locus_tag="OO544_003099"
     CDS             complement(5974..6882)
                     /locus_tag="OO544_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6768628.1"
                     /translation="MAFQVKIHQIRAFVEVARQGSIRGASRMLNMSQPALSKSIQELE
                     EGLAAQLFFRRSKGVTLTDAGESFYQHASLILEELRAAQEDIRQRQGQLAGQINIGMG
                     ASISRSLMPAVISRFHQQHPQVKVRIMEGQLVSMINELRQGELDFTINTYYQGPYDHE
                     FTFEKLLEKQFAIFCRPGHPAIGARSIKQLLDYSWTMPTPHGSYYKQLSELLDDQAQT
                     PQVGVVCETFSACISLVAKSDFLSILPEEMGCDPLHGQGLVMLPVSEILPKAAYYLIQ
                     RRDSRQTPLTASLITQFRRECGYLQS"
     gene            7058..8368
                     /gene="abgA"
                     /locus_tag="OO544_003100"
     CDS             7058..8368
                     /gene="abgA"
                     /locus_tag="OO544_003100"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgA"
                     /protein_id="HCR6768629.1"
                     /translation="MESLNQFVNSLAPKLSHWRRDFHHYAESGWVEFRTATLVAEELQ
                     QLGYSLALGREVVNESSRMGLPDELTLQREFERARQQGALAQWIAVFEGGFTGIVATL
                     DTGRPGPVMAFRVDMDALDLSEEQDVSHRPYRDGFASCNAGMMHACGHDGHTAIGLGL
                     AHTLKQFESGLHGVIKLIFQPAEEGTRGARAMVDAGVVDDVDYFTAVHIGTGVPAGTV
                     VCGSDNFMATTKFDAHFTGTAAHAGAKPEDGHNALLAAAQATLALHAIAPHSEGASRV
                     NVGVMQAGSGRNVVPTSALLKVETRGASDVINQYVFDRAQQAIQGAATMYGVGVETRL
                     MGAVTASSPSPQWVAWLQSQAAQVAGVNQAIERVEAPAGSEDATLMMARVQQHQGQAS
                     YVVFGTQLAAGHHNEKFDFDEQVLAIAVETLARTALNFPWTRGI"
     gene            8368..9813
                     /gene="abgB"
                     /locus_tag="OO544_003101"
     CDS             8368..9813
                     /gene="abgB"
                     /locus_tag="OO544_003101"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgB"
                     /protein_id="HCR6768630.1"
                     /translation="MQEIYRFIDDAIEADRQRYTDIADQIWDHPETRFEEFWSAEHLA
                     SALESAGFTVTRNVGNIPNAFIASFGQGKPVIALLGEYDALAGLSQQAGCAQPTSATP
                     GENGHGCGHNLRGTAAFAAAIAVKKWLEQYGQGGTVRFYGCPGEEGGSGKTFMVREGV
                     FDDVDAALTWHPEAFAGMFNTRTLANIQASWRFKGIAAHAANSPHLGRSALDAVTLMT
                     TGTNFLNEHIIEKARVHYAITNSGGISPNVVQAQAEVLYLIRAPEMTDVQHIYDRVAK
                     IAEGAALMTETTVECRFDKACSSYLPNRTLENAMYHALSHFGTPEWNSEELAFAKQIQ
                     ATLTPNDRQNSLNNIAATGGENGKAFALRHRETVLANEVAPYAATDNVLAASTDVGDV
                     SWKLPVAQCFSPCFAVGTPLHTWQLVSQGRTSIAHKGMLLAAKTMAATTVNLFIDSGL
                     LQECQQEHQQVTDTQPYHCPIPKNVTPSPLK"
     gene            9850..>10943
                     /locus_tag="OO544_003102"
     CDS             9850..>10943
                     /locus_tag="OO544_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HCR6768631.1"
                     /translation="MSMSSIPSSSQSGKLYGWVERIGNKVLHPFLLFIYLIIVLMVTT
                     AILSAFGVSAKNPTDGTPVVVKNLLSVEGLHWSLPNVIKNFSGFAPLGAILALVLGAG
                     LAERVGLLPALMVKMASHVNARYASYMVLFIAFFSHISSDATLVIMLPMGALIFLAVG
                     RHPVAGLLAAIAGVGCGFTANLLIVTTDVLLSGISTEAAAAFNPQMHVSVIDNWYFMA
                     SSVVVLTIVGGLITDKIIEPRLGQWQGNSDEKLQTLTESQRFGLRIAGVVSLLFIAAI
                     ALMVIPENGILRDPINHTVMPSPFIKGIVPLIILFFFVVSLAYGIATRTIRRQADLPQ
                     LMIEPMKEMAGFIVMVFPLAQFVAMFNWSN"
BASE COUNT         2739 a         2809 c         2715 g         2680 t
ORIGIN      
        1 tatcgtgaaa gagaagattc cagagggcaa aaccacttgc tcactgtgtt ctcgccttcg
       61 tcgcggtatc ctttatcgta ccgcaacgga actgggggcg acgaagatcg cgctgggtca
      121 ccatcgtgac gatatcctgc agacgctgtt cttaaatatg ttctacggcg gcaagatgaa
      181 aggtatgcct ccgaaactga tgagcgatga tggcaaacat atcgttattc gtccgctggc
      241 ctactgccgc gagaaagata ttcagcgatt tgccgatgca aaagcgttcc cgattattcc
      301 gtgcaacctg tgcggttcac agcctaacct gcaacgtcag gtgattgctg acatgttgcg
      361 tgactgggat aaacgttatc cagggcgtat cgagacgatg ttcagcgcga tgcagaatgt
      421 ggtgccgtcg catctgtgcg ataccaacct gttcgatttc aaaggcatca cccacggttc
      481 tgaagtggtt aacgggggtg atctggcgtt tgatcgcgaa gagatcccac tacaaccggc
      541 gggctggcag ccagaagaag atgaaaatca gttggatgag ttacggctga atgtggttga
      601 agtgaaataa ccaggatggc gcccgatgcg caagcgtatc gggctactct tatggaggcc
      661 ggataagacg cggccagcgt cgcatccggc aatcccgaat aagatgttta ctcttgcacc
      721 cggcaattca acatttcatt attttaataa ccgcacccgg ctcgtttttc ctttaatctt
      781 cccgccctgt aactgtttcc atgctttatg agcaacagcc tgacggaccg cgacatagac
      841 atgcgccgga tgcacggcga ttttgccaat atctgcgcca tcaagcccga tatctcctgt
      901 cagtgcacct aatacatcac ccgggcgcat tttggctttt ttcccaccat cgatacacaa
      961 cgttgccatt tctgcttcca gcggcacaat ggaactatta gctggcggcg tttgccagtt
     1021 aagttttatc tgcaacatgt cagaaatgat attggcccgc tgtgcttctt ccggagcaca
     1081 gaaactgatc gccagaccgc tatttcctgc acgagctgta cggccgatgc gatgtacatg
     1141 aacttcaggg tcccacgcca gctcaaagtt caccaccagc tcaagcgatt taatatccag
     1201 accacgcgca gcaacatcag tcgcaaccag tacacgggcg ctaccgttag caaaacgtac
     1261 cagggtctga tcgcgatcgc gttgctccaa atcgccgtgt aatgacaatg cactttgccc
     1321 tacttcattc agcgcgtcgc agacagcctg gcaatctttt ttggtattgc aaaacaccac
     1381 gcaagaggat ggctgatgca agcttaataa ccgttgcaac agaggaattt tgcctttgct
     1441 ggatgtctca taaaattgtt gttcaatggg tggcaaagca tctgttgagt caatttcaat
     1501 cgccaaagga tcgcgttgca ctcgtccgct gattacggca atggcttccg gccaggttgc
     1561 cgaaaacaga agcgtctgtc gagatgcagg cgcaaaacgg atgacatcat caatggcatc
     1621 gctaaatccc atatccagca tgcggtcggc ctcatccatc accaaagtat tcagcgcatc
     1681 cagtgatacc gtgccttttt gcaagtgatc cagcaaacga cccggcgttg ccacgataat
     1741 atgcggcgca tgttgcaacg aatcacgctg cataccgaac ggttgaccac cgcacaacgt
     1801 caaaattttg gtatttggca gaaaacgcgc cagccgacgc aattcacctg ccacctgatc
     1861 cgccagttca cgcgtaggac acagcactaa agcctgggtt tgaaatagcg acgcatcaat
     1921 ttgctgtaac aagccgaggc caaaagccgc cgttttgccg ctgccggttt tcgcctgcac
     1981 gcgaacatct tttccggcaa ggatcgccgg aagcgcggcg gcctgcaccg gcgtcatagt
     2041 taaataaccc aactcattaa ggttcgtgag ttgggcggga ggcaaaacat tcagggtaga
     2101 aaaagcggtc acaatctatt ctcgtggtca tcgacgcaaa gttagcaggc gcgtatcctc
     2161 gcagatctac gctcacgatg cgacaattta atcggttctt catcgggtgg tgggtcaggc
     2221 atgggttgcg ggcgagggat cggatcgggc actggaacag gatcgccagg aatcggttca
     2281 gggacaggaa tttgcaaata aataagtgtc gtcatatttc cctccggtca ttgggtggac
     2341 tcttaaaggg tagacgctga taaataacag gcaaaaaaaa gccgactcat caaagtcggc
     2401 gtcgtacgaa tcaattgtgc tatgcagtaa ttcaaaaaag gaagtaagac aatatggagc
     2461 gcaacgccca tcgcttgacg ttgcattcac ctgcaaggga gatattgccc tgaatgggta
     2521 gagagtttat tgacttcgct caaactttgc ggcgtttttg tatacagaca gccggaaaaa
     2581 ttgcttttgt tacaaccatt tactacggtg caaccataaa gcaacaccac caataagaac
     2641 aactaacaga atacaaaaaa ttgaaaatcc gaattgccac ccgccgccag ggatcccacc
     2701 aaggttgacg ccaaataacc cggtcagaaa ggtactgggt aaaaagacca ttgccatcaa
     2761 cgacattgta taggtacgac gagctaaatt ttcctgcatc acctgagcga tttcatccgc
     2821 catcacgcca gtccgtgcta tacaggcgtc gatttcgtca aggccgcgcc caaggcgatc
     2881 ggcaatatcc tgcatccgac ggcgttggtc atcgctcatc cacggcaaac gttcactggc
     2941 aagacgagca taaacatcac gttgcggtgc catatagcga cgcatcacaa ttaattgttt
     3001 gcgcagcaga gccaggaatc cacgcggtgg aatttgctga tcaaggagat tatcttcaag
     3061 gtcgataatt ttatcgtgca gctgctcgat aaattcgctg gaatgatcgg tcaacgcatc
     3121 gcacacatcc accagccacc ctccgcaatc ggtcggaccc gtgccctctt ccagatcgct
     3181 caccacatcg tccagcgcca gcactttgcg ttgtcgggtc gaaacaatta accgcccgtc
     3241 catatataca cgcatggcga ccagttgatc ggggcgttca tcggtgctgc cgtttataca
     3301 gcgcaatgta atcagcgtgc cttcaccgag acggctgact cggggccgcg tgctctcgcc
     3361 cgccagcgca tcacgtacgt tattgggaag cagcggtgtt gtcgccagcc attgggcgct
     3421 atcatggtgt acataattaa ggtggagcca gcagggatgc gcttcatcaa tcacatctgt
     3481 attttccagc ggtttaacgc cgcctctacc atccagcatc caggcaaata ctgcatccgg
     3541 gacattaacg tccgatccct taatcgcttc cacagtgcct ccatcatcaa cgcattattt
     3601 tgtagtctag ccttctggcc ctgttacgca acatctcatc accccattac cctgaaatga
     3661 ttaataaaat tctgtctaaa ttgaatacaa aaagcaaaat gcttttccgt atacaaaccg
     3721 tgtgaagtgt taaatagcgt ctatcattat cagaattatc tgatcatatg acgtggcttt
     3781 tttgcgatcg gatagcaaca aaaattgata aaaataacgg gatctcaatg attacgcaca
     3841 acttcaatac cctggactta ctcaccagtc ctgtctggat cgtttcgccc tttgaggaac
     3901 agttaattta tgccaatagc gcggcgcgac tgttgatgca agacctcacg tttagtcagc
     3961 tacgaaccgg tccctattcc gtctcctcac aaaaagaact gccgaaatac ctctccgatc
     4021 tgcaaaacca acacgatatt atcgaaatcc tcactgttca gcgtaaagaa gaggaaacag
     4081 cattaaactg tcggcttgtt ttgcgaaagc tgacagaagc agaaccggtg attattttcg
     4141 aaggtatcga agcgtcggca acgctgggtt taaaagccag tcgctcggca aattatcagc
     4201 gcaaaaaaca aggtttttat gcgcgctttt ttctgactaa ctctgcacca atgttgttga
     4261 ttgacccctc acgagatggc caaatcgtcg atgctaacct cgccgcgctc aatttctatg
     4321 gttataacca tgaaacgatg tgccagaaac atacctggga aataaatatg ctcgggcgtc
     4381 gcgtcatgcc tatcatgcat gaaatttcgc atttacccgg tggtcataaa cctctgaatt
     4441 ttattcataa gctggcggat ggttcgactc gtcatgtgca gacctacgcc gggccgattg
     4501 aaatttatgg cgacaagctc atgttatgta ttgtacatga tattactgag caaaaacggc
     4561 tggaggagca gctggaacat gctgcccacc atgacgcgat gaccggatta ctgaatcggc
     4621 gacagtttta tcacattacc gagccaggcc aaatgcagca tctcgccatc gcccaggatt
     4681 acagcttgtt gctcatcgac accgatcgtt ttaaacacat taacgatctc tatgggcatt
     4741 ctaaaggtga tgaggtgtta tgcgccctcg cccgcaccct cgaaagttgc gctcgcaaag
     4801 gcgatttggt gtttcgttgg ggaggcgaag agtttgtctt attgctacca agaaccccac
     4861 tggataccgc gctttcgctg gctgaaacta tccgcgtaag cgtggcaaaa gtgagtattt
     4921 cgggcttacc acgctttacc gtcagcattg gtgtggcgca tcacgaagga aatgaaagca
     4981 tcgatgaact gtttaaacgc gttgatgatg ctttgtatcg ggcgaaaaat gatggacgca
     5041 accgcgtgct ggcggcataa gccgcggatg cgtctcgaga tcaacgactg cgcttagcgt
     5101 ggcgctccca gttttcttgc ttcgcctgcg ccgttttacg tagtgcgacg taacacgccc
     5161 cgctgccgcc atgatgcggt agcgcagtgc aatatgcctg aacatcatca aattcggtca
     5221 gccagcgcgc cacatagctg cggacaatat tggcatgcga tttatcatcc cgtcctttgc
     5281 catgaataat cagcacgtta cgcaaaccat cagccatggc ttgttgaatg aaactgaaca
     5341 tcattttgcg gcactcttcc accggctggc gcaaaagatt caggctcgcc tgttgcggat
     5401 atttaccact gcgcagctta tccagcaccc catgttgcaa cccttcccgc cgaaactcca
     5461 gcggctgact tagtgggatg atgtcgagaa atccggtggt gaggaaatta tcaagctgca
     5521 gcgtgtcgat acgctgcggc gcacgttggt tacgcgttgg atgccagtgg acatcggtag
     5581 cacgtttcag cggctggaca tcttccatgg cgtcaagaaa cagcgatttg tcgtcaaggt
     5641 tcatgttaca tcctcccgca attaagagcg cgatatgata accagaccgg gtcggtccaa
     5701 caacgtatta cccaaaattc cagtaataag ttccaaatat tgccgatatt ttaagcaaaa
     5761 tgcttatgca tgattattca ttcacgatat taataatgta acttatattt tcgtgaaatc
     5821 tgtcactgaa gaaaattggc aactaaaggt taaaaccgtt ataacacagt caccggcgca
     5881 gaggagacaa tgccggattt aagacgcgga tgcactgctg tgtgtactgt agagtctggc
     5941 ggatgtcgac agactctatt tttttatgca gttttaactt tgcagatagc cgcattctcg
     6001 ccggaattgc gtgattaatg acgcggtcag tggtgtctgg cgactatcac gccgctgaat
     6061 caaataatag gccgctttcg gtaaaatttc gctaaccggc aacatcacca gcccctgtcc
     6121 gtgcaaggga tcgcagccca tttcttcagg cagtatgctg agaaaatcgc tttttgccac
     6181 cagactgata caggctgaga acgtctcgca gactacaccg acctgtggcg tttgcgcctg
     6241 atcgtcaagc aattcactca actgtttgta gtaactgccg tgtggcgtcg gcattgtcca
     6301 gctgtaatcc agtaactgtt tgatcgaacg ggcaccaatg gcggggtgtc ccgggcggca
     6361 aaagatcgcg aattgctttt ccagtaattt ctcaaaagta aattcgtggt cgtacggtcc
     6421 ctgataatag gtattgatgg tgaaatccaa ttctccctga cgcaattcat taatcatcga
     6481 caccagttgc ccttccataa tgcgtacttt tacctgcgga tgctgctgat gaaaacgaga
     6541 tatgacagct ggcatcagac tgcgggaaat actggccccc atgccgatat taatctgccc
     6601 tgccagttgc ccttgtcgtt ggcgaatatc ctcttgggct gcgcgcagct cttcaagaat
     6661 aagactggcg tgctgataaa aactttcacc ggcatcagtt aacgtcacgc ctttactacg
     6721 gcgaaaaaag agttgcgccg ctaatccttc ttccagctcc tgaatagatt tactcagtgc
     6781 cggttgcgac atattcaaca ttcggctcgc tccgcgaatg ctgccctgac gagccacttc
     6841 aacaaaagcc cgaatttgat gaatttttac ctgaaaagcc atgacgccac cgataaccgt
     6901 tatttatcag accaaagaaa ctggcatcta ctttaatgca gatgattgtg tcagggtaat
     6961 ttatgaacgg ttaaaactgt gaaaaatcag ttagtgataa gtaaaaacta tcgctacgtg
     7021 aaccgggtca cactttttac tgatgacggg aaaggttatg gagtctttga atcaatttgt
     7081 taattcgctt gccccaaaat tatcgcactg gcgacgtgat tttcatcact atgcagagtc
     7141 tggctgggtg gaattccgca ctgccaccct tgttgcggaa gaattgcagc agctcggcta
     7201 ttcactggcg ctgggccgcg aagtagttaa tgaaagtagc cggatgggac tacctgatga
     7261 actcactcta caacgcgaat tcgagcgcgc tcgtcaacag ggtgcgctag cacaatggat
     7321 tgcggttttt gaaggtggtt tcactggtat cgtcgccacc ctggataccg gtcgccccgg
     7381 tccggtgatg gctttccgtg tcgatatgga cgcgctggat ctcagtgaag agcaggatgt
     7441 cagccatcgc ccctaccgcg acggttttgc gtcatgtaac gccggaatga tgcatgcctg
     7501 tggtcatgat ggacataccg ccattgggct tgggctggcg cataccctta aacaattcga
     7561 gtccggacta catggcgtca tcaaactgat ttttcagcct gcagaggaag gtacgcgtgg
     7621 cgcgcgggcg atggtcgatg caggtgtcgt agatgatgtt gattatttta ctgccgtgca
     7681 cattggcact ggcgtacctg cgggcaccgt ggtgtgcggc agtgataatt ttatggcaac
     7741 caccaaattt gacgcgcact tcaccggtac cgccgctcac gcaggcgcaa aaccagaaga
     7801 cggtcacaat gccttgttgg cggcagcaca agccactctt gcactgcatg caatcgcccc
     7861 gcacagcgaa ggagcttcca gagtaaacgt gggcgttatg caggcaggaa gcggtcgtaa
     7921 cgttgttcct acctcggcgt tgctgaaagt ggaaacacgc ggggccagcg acgtcattaa
     7981 tcaatatgtt tttgaccgtg cacaacaagc gattcagggc gcagcaacca tgtatggtgt
     8041 cggcgttgaa actcgtctga tgggtgcagt taccgccagt tctccttcgc cgcaatgggt
     8101 cgcatggttg caaagtcagg cggctcaggt cgcgggggtc aatcaggcca ttgaacgtgt
     8161 tgaagcgcct gcgggttccg aagatgccac attaatgatg gcccgcgtgc agcaacatca
     8221 agggcaagcc tcctacgtgg tgtttggcac acagctggcg gcaggtcatc acaacgaaaa
     8281 attcgatttt gacgagcagg ttctcgctat tgccgtcgaa acgctggcgc gcaccgcgct
     8341 caattttccc tggacgcgag gtatctgatg caggaaatct atcgttttat cgacgatgcg
     8401 attgaagccg atcgccaacg ttataccgat attgccgatc aaatctggga tcatccagaa
     8461 acacgttttg aagagttctg gtcagcggag catctggctt cggctctgga atctgcaggc
     8521 ttcaccgtta cccgcaacgt aggcaatatc ccaaatgcct ttattgcttc gtttggtcaa
     8581 ggcaaaccgg ttatcgccct gctgggagaa tatgacgccc tggcaggttt aagtcagcaa
     8641 gctggttgcg cgcaacctac atccgcgacg cccggtgaaa atggtcacgg ttgcggacac
     8701 aatttgcggg gaaccgccgc ctttgccgct gcaatagccg tcaagaaatg gctggaacaa
     8761 tatgggcaag gcggcacagt gcgcttttat ggttgtcctg gcgaagaagg cggctcgggt
     8821 aaaacgttca tggtccgcga gggggtattt gatgatgtgg atgcggcact cacctggcac
     8881 ccggaagcct ttgccggtat gttcaatacc cgcacgctgg caaacattca ggcatcatgg
     8941 cgctttaaag ggatcgcagc acatgccgcg aattcccctc atttgggacg cagcgccctt
     9001 gatgccgtaa cgttgatgac cactggcacc aacttcctca acgaacatat tattgaaaaa
     9061 gcgcgcgtac actatgccat cacaaatagc ggcgggatct cgcccaacgt ggtccaggcg
     9121 caggcagaag tgctttatct tatccgcgcc cccgaaatga ccgacgtgca gcatatttat
     9181 gatcgggtcg ccaaaatcgc cgaaggtgcg gcattgatga ccgaaaccac ggttgaatgc
     9241 cgcttcgaca aagcctgttc cagttatctc ccgaatcgca ccttagaaaa tgccatgtac
     9301 catgccctat cccattttgg taccccggaa tggaactccg aagaactggc ttttgcgaaa
     9361 caaattcagg ctacgctcac ccccaacgat cggcaaaaca gtctgaataa tatcgccgca
     9421 accggtggcg aaaacggcaa ggcttttgca ctacgtcatc gtgaaacggt actggcgaat
     9481 gaggtcgctc catatgccgc caccgataac gtgcttgcgg catcgactga tgtcggcgac
     9541 gttagttgga aactgcctgt tgcccagtgt ttcagcccct gttttgccgt cggtacaccg
     9601 ctacatacgt ggcaactggt tagccagggg cgaacatcta ttgctcataa aggaatgctg
     9661 ctggcggcga aaactatggc agcaaccaca gtcaatctct tcattgattc agggctattg
     9721 caagaatgcc aacaagagca tcagcaagta acggacacgc aaccgtatca ctgccctatc
     9781 ccgaaaaacg tgacaccgtc acctttaaaa taacaacaac aacgcaaaca caacaaccga
     9841 ggaatgccca tgagtatgtc atccataccg tcgtcctccc aatccgggaa gctctatggc
     9901 tgggtcgaaa gaattggtaa caaggttctc catccttttt tgctctttat ctatttgatt
     9961 atcgtactca tggtgacgac ggcaattttg tcggcctttg gcgtcagtgc gaaaaacccg
    10021 accgatggaa cgccggtggt ggtgaaaaac ctgctcagtg tggaaggatt acactggtct
    10081 ttacccaatg ttattaaaaa ctttagcggt tttgctccac ttggtgcgat cctggcgctg
    10141 gttttaggtg ccggtctggc ggagcgcgtc ggcttactgc cagcgctaat ggttaaaatg
    10201 gcatcgcatg ttaatgcccg ctacgccagt tatatggtgc tgtttattgc ttttttcagc
    10261 cacatttctt ccgatgcgac gttagtgatc atgctaccga tgggtgcgct gatttttctg
    10321 gcggtgggca ggcatccagt tgcaggttta ctggctgcca ttgcaggcgt aggttgcggc
    10381 tttacggcta atttactgat tgtcacaacc gacgtgttgc tgtcggggat cagcacggaa
    10441 gcggcggctg cgttcaatcc gcaaatgcac gtcagtgtaa ttgataactg gtattttatg
    10501 gccagctccg tagtcgtact gacgattgtt ggcggcctga taaccgacaa aatcatcgag
    10561 ccacggttag gtcaatggca gggaaacagc gatgagaaac tgcagacatt gaccgaaagt
    10621 cagcgttttg gtttacgcat agcaggtgtc gtatcgctac tttttattgc tgcgattgcg
    10681 ctgatggtga tcccggaaaa cgggatattg cgcgatccga ttaatcacac cgtgatgcca
    10741 tcacccttta ttaaaggtat cgtgccactg atcattcttt ttttctttgt tgtctcgctg
    10801 gcttatggca tcgctacccg cacaattcga cgtcaggcgg atttaccgca gttaatgatt
    10861 gaaccgatga aagagatggc aggatttatc gtgatggttt ttcccctcgc ccagtttgtc
    10921 gccatgttta actggagcaa cat
//
LOCUS       DAJTZL010000136        10928 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.136, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000136 DAJTZL010000000
VERSION     DAJTZL010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10928
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.136"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..497
                     /gene="radC"
                     /locus_tag="OO544_003103"
     CDS             <1..497
                     /gene="radC"
                     /locus_tag="OO544_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HCR6768632.1"
                     /translation="LTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            714..950
                     /gene="rpmB"
                     /locus_tag="OO544_003104"
     CDS             714..950
                     /gene="rpmB"
                     /locus_tag="OO544_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HCR6768633.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            971..1138
                     /gene="rpmG"
                     /locus_tag="OO544_003105"
     CDS             971..1138
                     /gene="rpmG"
                     /locus_tag="OO544_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCR6768634.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            1236..2045
                     /gene="mutM"
                     /locus_tag="OO544_003106"
     CDS             1236..2045
                     /gene="mutM"
                     /locus_tag="OO544_003106"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HCR6768635.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(2084..2563)
                     /gene="coaD"
                     /locus_tag="OO544_003107"
     CDS             complement(2084..2563)
                     /gene="coaD"
                     /locus_tag="OO544_003107"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HCR6768636.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALTQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALVAKLA"
     gene            complement(2571..3848)
                     /gene="waaA"
                     /locus_tag="OO544_003108"
     CDS             complement(2571..3848)
                     /gene="waaA"
                     /locus_tag="OO544_003108"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HCR6768637.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            4261..5319
                     /gene="rfaQ"
                     /locus_tag="OO544_003109"
     CDS             4261..5319
                     /gene="rfaQ"
                     /locus_tag="OO544_003109"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HCR6768638.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTTPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            5316..6440
                     /locus_tag="OO544_003110"
     CDS             5316..6440
                     /locus_tag="OO544_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCR6768639.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYHLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            6433..7230
                     /gene="rfaP"
                     /locus_tag="OO544_003111"
     CDS             6433..7230
                     /gene="rfaP"
                     /locus_tag="OO544_003111"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HCR6768640.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGI
                     NPLSRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAVGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFVAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            7246..8262
                     /gene="waaO"
                     /locus_tag="OO544_003112"
                     /gene_synonym="rfaI"
     CDS             7246..8262
                     /gene="waaO"
                     /locus_tag="OO544_003112"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     3-alpha-galactosyltransferase"
                     /protein_id="HCR6768641.1"
                     /translation="MSAHYFNPQEMINKTIIFDERPAASVASSFHVAYGIDKNFLFGC
                     GVSITSVLLHNNDVSFVFHVFIDDIPEADIQRLAQLAKSYRTCIQIHLVNCERLKALP
                     TTKNWSIAMYFRFVIADYFIDQQDKILYLDADIACQGNLKPLITMDLANNVAAVVTER
                     DANWWSLRGQSLQCNELEKGYFNSGVLLINTLAWAQESVSAKAMSMLADKAVVSRLTY
                     MDQDILNLILSGKVKFIDAKYNTQFSLNYELKKSFVCPINDETVLIHYVGPTKPWHYW
                     AGYPSAQPFINAKEASPWKNEPFMRPINSNYARYCAKHNFKQNEPINGIMNYIYYFYL
                     KIIK"
     gene            8279..9274
                     /locus_tag="OO544_003113"
     CDS             8279..9274
                     /locus_tag="OO544_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide 1,2-glucosyltransferase"
                     /protein_id="HCR6768642.1"
                     /translation="MNEFIKERFSYLADNKKENAPELNVSYGIDKNFLYGAGVSISSV
                     LINNSDINFVFHVFTDYVDDDYLKSFNETAKQFNTSIIVYLIDPKYFADLPTSQFWSY
                     ATYFRVLSFEYLSESISTLLYLDADVVCKGSLKPLTEIIFKDEFAAVIPDNVSTQAAC
                     AKRLNIPEMNGRYFNAGVIYVNIKKWHEANLTPYLLTLLRGETKYGSLKYLDQDALNI
                     AFNMNNIYLAKDFDTIYTLKNELHDRSHRKYQQTITDKTVLIHYTGITKPWHSWAGYP
                     SASYFNIAREQSPWKKYPLKEARTVAEMQKQYKHLFAHGEYIKGITSLIKYKLKK"
     gene            9284..9976
                     /gene="rfaY"
                     /locus_tag="OO544_003114"
     CDS             9284..9976
                     /gene="rfaY"
                     /locus_tag="OO544_003114"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HCR6768643.1"
                     /translation="MITSIRYRGFSFYYKDNDNKYKEIFDEILAYNFKTVKVLRNIND
                     TKVSLIDTKYCRYVFKVFAPKTKRNERFLKSFVKGDYYQNLIVETDRVRSAGLTFPND
                     FYFLAERKIFNYASVFIMLIEYVEGVELNDMPIIPEDVKAEIKASMEKLHALNMLSGD
                     PHRGNFIVSKDGVRIIDLSGKSCTAERKARDRLAMERHLGIANEIKDYGYYSVIYRTK
                     LRKFIKKLKGKA"
     gene            10002..>10928
                     /locus_tag="OO544_003115"
     CDS             10002..>10928
                     /locus_tag="OO544_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCR6768644.1"
                     /translation="MDLLAESITEVAVSGEIANTDRVLNIAYGIDRNFLFGAAVSMQS
                     VVMHNPDLAVKFHLFTDYIDEDYLQRVNAFTSKNANVEVRIYKVSSAFIDIFPSLKQW
                     SYATFFRLVAFQYLSETIENLLYIDADVICKGSLAGLLDINFDGDKFAAVIKDVPFMQ
                     EKPAKRLAIEGLPGNYFNAGVVYLQLEAWAKNDFMNKAIAMLASDPQHTKYKCLDQDI
                     LNILFFGHCIFISGDYDCFYGIDYELKNKSDEDYKKTITDDTKLIHYVGVTKPWNDWT
                     NYPCQKYFNEAYQASCWNDVAFIPATNEKQYQV"
BASE COUNT         2985 a         2344 c         2601 g         2998 t
ORIGIN      
        1 tgttaacctc tgaatatgaa caatttagtg gcgttcatgg aattggcgta gcgaaatttg
       61 cccagttaaa ggggattgct gaactggcgc ggcgttacta caacgtgcgg atgcgtgaag
      121 aaagcccttt actcagcccg gagatgacgc gggaattttt acaaagccag ctcacgggtg
      181 aggagcggga gatctttatg gtgatctttc tcgactcaca acaccgggtt ataacgcata
      241 gccgtctttt ttccggcacg ctaaaccatg ttgaagtcca tcctcgggaa attatccggg
      301 aagcgataaa aataaacgcc tcggcgctga tccttgcaca taatcaccct tcgggttgtg
      361 ctgaacccag taaagcggat aaactcatta ctgaacggat aataaagagt tgtcagttca
      421 tggatttacg cgtgctcgac catatcgtga ttgggcgtgg agagtatgtt tcttttgccg
      481 aacgcggctg gatttaaccc gctatgcgcg atccttcggg atctttgtct gttcgggact
      541 tgagcacatc gctgagtcag cgtatactac gccacctttg agaatctcgg gtttggcatt
      601 tgggcctggc aatcgagagt tcacatagaa ctgcgatgac cgggctgtaa agcctgacga
      661 ggcgccaata ccccatacga agctcgagct aatttgattt ttggagaata gacatgtccc
      721 gagtctgcca agttactggc aagcgtccgg tgaccggtaa caaccgttcc cacgcactga
      781 acgcgactaa acgccgtttc ctgccgaacc tgcactctca ccgtttctgg gttgagagcg
      841 aaaagcgttt tgtcaccctg cgcgtatctg ctaaaggtat gcgtgtaatc gataaaaaag
      901 gcatcgatac agttctggct gaactgcgtg cccgtggcga aaagtactaa gtacttagag
      961 gaaataaatc atggctaaag gtattcgtga gaaaatcaag ctggtttctt ctgctggtac
     1021 tggtcacttc tataccacta cgaagaacaa acgtactaag ccggaaaaac tggaactgaa
     1081 aaaattcgat ccagttgttc gccagcacgt gatctacaaa gaagcgaaaa tcaaataatt
     1141 ctcgctttga tgtaacaaaa aacctcgctc cggcggggtt ttttgttatc tgcttgcccc
     1201 catattgatt gcatctgttc attcctggag atgctatgcc tgaattaccc gaagttgaaa
     1261 ccagccgtcg cggcatagaa ccgcatctcg ttggtgcaac cattcttcat gcggtggtgc
     1321 gcaacggacg cttgcgctgg ccggtttcag aagagatcta ccgtttaagc gaccaaccag
     1381 tgcttagcgt gcagcggcgg gctaaatatc tgctgctgga gctgcctgag ggctggatta
     1441 tcattcattt ggggatgtct ggcagcctgc gcatccttcc agaagaactt ccccctgaaa
     1501 agcatgacca tgtggatttg gtgatgagca acggcaaagt gctgcgctac accgatccgc
     1561 gccgctttgg tgcctggctg tggaccaaag agctggaagg gcacaacgtg ctggctcatc
     1621 ttggaccgga gccgcttagc gacgatttca atggtgagta tctgcatcag aagtgcgcga
     1681 agaaaaaaac ggcgattaaa ccgtggctga tggataacaa gctggtggta ggggtaggga
     1741 atatctatgc cagcgaatca ctgtttgcgg cggggatcca tccggatcgg ctggcgtcat
     1801 cactgtcgct ggcagagtgt gaattgttag ctcgggtgat taaagcggtg ttgctgcgtt
     1861 cgattgagca gggtggtaca acgctgaaag attttctgca aagtgatggt aaaccgggct
     1921 atttcgcgca ggaattgcag gtttacggac gaaaaggtga gccgtgtcgg gtgtgcggta
     1981 cgccgattgt ggcgactaaa catgcgcagc gggcaacgtt ttattgtcgg cagtgccaga
     2041 agtaattcat acgcgccgga tggcatacca tccggcataa acgctacgct aacttcgcca
     2101 ccagcgcctg atggacattc tccggcagga aatgggtgac atcgccctga tggcgcgcca
     2161 cctctttcac caacgatgaa gagataaacg accactcttt cgacggcatc agaaacacac
     2221 tttccagttc cggcattaag tggcgattca tatgcgccag ctgcatttca tattcaaaat
     2281 ccgccaccgc acgcaggcca cgaatcagca ccgtagcgtg ttgattacgg gcgaagttcg
     2341 ccatcaaatc actaaaccca accacttcca cgttccccag atgcgcggtt gcctgctgtg
     2401 tcagcgccac acgctcttcc agggtaaaca tcggtttttt actggggctg gcggcaatcg
     2461 ccagaataac gtgatcgaac atttgcgtgg cgcgcgtcac gatatcgata tgaccattgg
     2521 taatgggatc gaaagtaccc ggataaatcg cccgtttttg cataacaacc tcaatgcgtt
     2581 ttcggtggca ggtaaggttc cagcagttga agcagacgct gtagcgcgcc ctggttttga
     2641 tacagtactt caacggcatg acggccatag aaactacggt aatcggcgtc ggtgagtaaa
     2701 gaggaaacct cttttgcaag cgtagtggca tcggtaacgg taatcagccc gcttgcctgc
     2761 tccagccgcg cgcaaatgtc tttgaaatta aaagtatgcg gccccatcaa taccggaata
     2821 gcgtgtgcgg cagcttccag aggattatgc cctccacgtt caaccagtga accgccaaca
     2881 aaggcgagat cggcaatgcc gtacagtaac atcaactcgc ccatcgtatc gccaaccaca
     2941 acctgcgtgc tggtggaggg gacttcccct gaagagcgtg tgatatagct tagtccagcc
     3001 tggcggacaa ggttaatcgc atccgggaag cgttctggat gacggggtac caggatgagc
     3061 aataaattcg ggaattgctg taataatgcc tgatgtgcgg cgatcaccac actctcttca
     3121 ccttcgtgag tgctggtggc aatccatacc gggcggtgtg gtgcccactg gcggcgcagc
     3181 gtcacggctt tagcagccaa ctgcggcgtt acagaaatat cgaatttcag gctaccggta
     3241 acggttacct ggttattttt tgcgcccagc gccacaaaac gtgcaccatc ttcttcattt
     3301 tgcgcagcaa tcagcgtaat acgacgcagt aagcggcgga cgaatttacc cagtttggca
     3361 taacctgcgg ccgagcgggc agagagtcgc gcgttagcga tcaccagcgg aattttacgt
     3421 ttatgtagcg ccgcaatcag gttaggccat agttcggttt ccataatcaa caccagtttg
     3481 gggtcgactt tattcaggaa acggttgagc gcatcgggca ggtcatacgg cagataaacg
     3541 tgctgaacat ccttcccgaa agccgattgt acgcgctccg aaccggttgg cgtcatggtt
     3601 gtaacggtaa tcggtaaatc aggataacga tggcgcagcg cgcgcaccaa cgggatcgcc
     3661 gccagagttt caccgacgga gacggagtgc agcataatgc cgcctggttt tagcggatgg
     3721 cggtaaaaac cgtaacgttc accccagcgt tttcgatagg ccggagcctt acgtccgcgc
     3781 acccagagcc gtatccagat cagcggctga ataaggtaga gaagggcggt gtaaagcaat
     3841 tcgagcatag taaatagctg acttatggat gtgctgggga ttctatgtat ttagctgtgg
     3901 ctttaccatt acttttcccg tttttgactt aaatagcaat ttacccgcca attcttgctg
     3961 aacatctata ctttttttgt atttttatgc gattcattga tttctgctgt acgtgcagga
     4021 aggaatcgca gagcaacagt gaaagaatat ggttgctaat gattttattt gattgatttc
     4081 tttattgaaa atcaacaatg tgcttaaaaa gcattcgggt cggattgccg cgaaagaagt
     4141 gatacactag cgcgtcaata tcagtgcggt atcagtattc aggtagctgt tgagcctggg
     4201 gcggtagcgt gctttttctg cttcacctta atcggataat ctcaacaaaa agagttactt
     4261 gtggataagc catttcgaag aattttgctc attaagatgc gttttcatgg ggatatgtta
     4321 ttaactactc ccgtcattag ttcgctgaaa aaaaattacc ctgacgcaaa aatcgatgtg
     4381 ctgctttatc aggacaccac cccgatcctg tctgaaaatc cagagattaa cgcgctctac
     4441 ggcataaaaa ataaaaaagc aaaagcctca gaaaaaattg ccaacttttt tcatctcatc
     4501 aaggtattac gtgccaataa gtatgacctt atcgtcaatc ttaccgatca atggatggtt
     4561 gctatactgg ttcgcttatt aaatgcccgt gtgaaaattt cccaggatta tcatcatcgg
     4621 cagtctgctt tttggcgtaa aagtttcacc catttagtgc cgttgcaggg tggaaatgtg
     4681 gtggaaagta acttatccgt gctgacacca ttgggacttg attcgttggt gaagcagaca
     4741 accatgagtt acccgcctgc aagctggaaa cgtatgcgtc gcgaacttga tcacgctggt
     4801 gttggacaaa attatgtggt tatccaacct acggcgcggc aaatcttcaa atgctgggac
     4861 aacgccaagt tttccgctgt gattgatgcc ttacatgctc gtggttatga agtcgttctg
     4921 acgtccggcc cagataaaga cgatctggcc tgcgtcaatg aaattgcgca gggatgccag
     4981 acgccaccag taacggcgct ggctggaaag gtgaccttcc cggaacttgg tgcgttaatc
     5041 gatcatgcgc agctgtttat tggcgttgat tccgcaccgg cgcatattgc cgctgcagtt
     5101 aatacgccgc tgatatcgct gtttggtgcg acagaccata ttttctggcg tccttggtca
     5161 aataacatga ttcaattctg ggcgggagat taccgggaaa tgccaacgcg cgatcagcgt
     5221 gaccgaaatg agatgtatct ttcggttatt ccggcggcag atgtcattgc tgctgtcgat
     5281 aaattactgc cctcctccac gacaggtacg tcgttatgat cgttgctttt tgtttatata
     5341 aatattttcc ctttggcggt ttgcagcgcg attttatgcg tattgctcag acagtcgccg
     5401 cccgaggtca tcatgttcgg gtttataccc agtcgtggga aggcgaatgc cctgatgtat
     5461 ttgaactgat caaagtgccg gttaaatcgc ataccaatca cgggcgcaat gcggagtatt
     5521 ttgcctgggt gcaaaaacat ttacgcgaac atcccgtcga taaagtcgtt ggattcaaca
     5581 aaatgccggg gctggacgtt tattatgccg ctgatgtttg ttatgccgag aaagtcgcgc
     5641 aggaaaaagg ctttttctat cacctgacgt cacgttatcg ccattatgcc gcctttgagc
     5701 gggcaacctt cgaacagggc aagccgacac agctgctgat gctgacagat aagcaaatcg
     5761 ccgatttcca gaaacattat cagactgaag cggagcgttt tcatattctg ccaccgggga
     5821 tttatcctga tcgtaaatat agccagcagc cagctaatag ccgtgaaatc ttccgtaaga
     5881 agaatggaat aaccgaacaa caatatttat tgttgcaggt cggttcagac ttcacgcgta
     5941 aaggtgtcga tcgttccatt gaagcacttg cttcgttacc ggattcgctg cgccacaaca
     6001 cattgctata tgttgttgga caggataagc cgcgaaaatt tgaggcgctg gcagaaaaac
     6061 gcggcgtgcg cagtaatgtt cacttcttct cggggcgcaa cgatgtctcg gaattaatgg
     6121 cggcggcgga tttattactg catcctgcct accaggaagc agcgggaatt gtgctactgg
     6181 aagcgattac tgcaggatta ccggtactaa caacagccgt ttgtggctat gcgcattata
     6241 ttgtcgacgc caattgcggc gaggctattg ctgagccatt ccgccaggaa acattgaatg
     6301 agattttacg caaagcgtta acgcaatctt cattgcgcca ggcctgggcg gaaaatgcgc
     6361 gacattatgc tgatacacaa gatttataca gtctgccaga gaaagcggcg gatatcataa
     6421 cgggtggtct ggatggttga acttaaagag ccgtttgcca cgttatggcg cggtaaagat
     6481 ccttttgagg aagttaaaac cttgcagggt gaggtttttc gtgaactgga aactcgccgc
     6541 actctgcgat ttgaaatggc gggcaaaagc tattttctca aatggcatcg cggcacgacc
     6601 ctgaaagaga taatcaaaaa tttactctca ttgcggatgc cagtattagg cgcagaccgc
     6661 gaatggaatg cgattcatcg actgcgggat gtcggcgttg atactatgta tggggtggca
     6721 tttggcgaaa aaggtattaa tccgctgagc agaacctcgt ttattattac cgaagatctg
     6781 accccaacca tcagtctgga agattactgt gctgactggg cgaccaaccc accagatgtt
     6841 cgcgtaaagc gtatgcttat taagcgtgtc gcgacgatgg tgcgcgatat gcatgctgtg
     6901 ggcattaacc accgtgactg ttatatctgt catttcctgc tgcacttgcc tttttccggt
     6961 aaggaagagg agttaaaaat ttcggtaatt gacctgcacc gggcgcagct tcgcacgcgc
     7021 gttccacgtc gttggcgcga taaagatctt attgggcttt atttttcttc gatgaatatc
     7081 ggcctgactc agcgggatat ctggcggttt atgaaagtgt attttgtcgc cccgcttaaa
     7141 gacattctca agcaggaaca aggactgctg tcgcaagcag aagcaaaagc cacaaaaatc
     7201 agggaaagaa cgattcgaaa atcgttgtaa tttccggaga gaaatatgag tgcccactat
     7261 tttaatccac aagagatgat caataagaca atcatcttcg atgaaaggcc agcggcgtca
     7321 gtggcatcat cattccatgt tgcttatggc attgataaaa actttctttt tggttgtggt
     7381 gtttcaatca cgtcagtttt gttacataac aacgacgtga gttttgtttt ccacgttttt
     7441 attgatgata tccctgaagc cgatatccag cgtttagccc aattggcgaa aagctatcgt
     7501 acctgtatcc agatccatct ggtaaattgt gaacggctta aggcattacc gacgaccaaa
     7561 aattggtcta ttgccatgta tttccgtttt gtaattgcag attactttat tgatcaacaa
     7621 gataagatcc tttacctgga tgctgatatc gcctgtcagg gaaacttaaa gccgctgata
     7681 acaatggatc ttgccaataa cgttgctgct gttgttactg aacgcgatgc taactggtgg
     7741 tcgttacggg gtcaaagtct gcagtgtaat gaacttgaaa agggttactt taattcaggt
     7801 gtcctgttaa ttaatacact agcgtgggcg caggagtccg tttctgctaa agcgatgtcg
     7861 atgcttgctg ataaagccgt cgtttcccgt ttaacctata tggatcaaga tatacttaat
     7921 cttatcctgt cagggaaagt taaattcatt gatgctaaat acaatactca atttagttta
     7981 aattatgaat taaaaaaatc atttgtttgt ccaattaatg atgaaaccgt attaattcat
     8041 tatgtcggcc cgacaaaacc ctggcattac tgggccggtt atccaagtgc gcaacctttt
     8101 atcaacgcca aagaagcatc gccctggaaa aatgaaccgt ttatgcggcc aattaattca
     8161 aactatgctc gttattgcgc caagcataat tttaaacaaa atgaaccaat taacgggata
     8221 atgaattata tttattattt ttatttaaag ataataaaat gatattgaga aaagttaaat
     8281 gaatgaattt ataaaagaac ggttttcgta tttagcagat aataaaaaag aaaacgcccc
     8341 agaactaaat gtttcctacg gtatcgataa gaattttttg tatggtgctg gcgtttcaat
     8401 ttcttccgtt ttgattaata attcagatat taattttgtc tttcatgttt tcactgatta
     8461 tgtggatgat gattatttaa agtcatttaa tgaaacagca aaacaattta atacctcaat
     8521 tattgtatat ttaattgacc caaaatactt tgctgatctg ccgacgtcac agttttggtc
     8581 gtacgcgaca tacttcaggg tattgtcctt tgaatatctg agtgaaagta tttccacact
     8641 gctgtatctg gatgccgatg tcgtttgtaa aggaagtctg aagcctctca cagaaattat
     8701 atttaaagat gagtttgctg cggtcattcc tgacaatgtt agtactcagg cggcatgtgc
     8761 aaaacgcctc aacattcccg agatgaatgg acgttatttc aatgcaggcg ttatctatgt
     8821 caatattaaa aaatggcatg aagcaaattt gacaccgtat ttactcacgc ttttacgagg
     8881 ggaaactaaa tatggctctc ttaaatattt agatcaggat gcgttgaata tcgcatttaa
     8941 tatgaataat atctacctcg cgaaggattt tgatactatt tataccctga aaaacgaact
     9001 tcatgatcgt agtcatcgaa agtatcagca aaccattacc gataaaacag tattgattca
     9061 ctatacaggg ataactaaac catggcatag ctgggctggt tatccgtctg catcatactt
     9121 taatatcgcg cgtgaacaat ctccctggaa gaaatatcct cttaaagagg cgcggactgt
     9181 tgcagaaatg cagaaacaat ataagcatct gtttgcccat ggtgagtata ttaaaggcat
     9241 aacttcatta attaagtaca agcttaagaa ataaattact cttatgatta caagtatacg
     9301 ctatcgcggc ttctcatttt attacaaaga taatgataat aaatataaag aaatctttga
     9361 tgaaatactg gcctataatt ttaaaacagt aaaagtatta cgtaatatta atgataccaa
     9421 agtatcattg attgatacga aatattgtcg ctatgttttt aaagtctttg ccccgaaaac
     9481 gaaaaggaat gaacgttttt taaaatcttt tgtaaaaggt gactactatc agaatttgat
     9541 cgttgaaacc gatcgtgtca gaagtgcagg tcttactttt cctaatgatt tttattttct
     9601 ggctgagcgt aaaattttca actatgccag tgtttttatc atgcttatcg agtatgtcga
     9661 gggcgtagag ctcaatgata tgccgatcat tccagaagat gttaaagcag aaattaaggc
     9721 ttcaatggaa aaattgcatg ccttaaatat gctatcgggc gacccacacc ggggtaactt
     9781 tatcgttagt aaagatggtg tcaggataat tgacctatcc ggtaaaagct gtacggcaga
     9841 gcgtaaagct cgtgatcgtc tggccatgga acgtcacctg ggtattgcca acgaaataaa
     9901 agattatggc tactattcag taatctacag aaccaaacta cgcaaattta ttaaaaaatt
     9961 aaaaggcaaa gcgtaaacca cacagtcaaa acggaaccaa catggattta ttagctgaga
    10021 gtattactga agtcgctgtc tctggggaaa ttgctaacac cgatcgtgtg ttaaatatcg
    10081 cttacggtat tgaccgtaac tttttatttg gtgcggcagt atctatgcaa tcagttgtta
    10141 tgcataatcc ggaccttgcg gttaagtttc atctctttac cgactacatt gatgaagatt
    10201 atctacaacg tgttaatgct tttaccagca aaaatgctaa cgttgaagta agaatttata
    10261 aagtctccag tgcctttatt gatatcttcc ccagcctgaa acagtggtct tatgcaacat
    10321 tcttccgttt agttgcgttc cagtatctga gtgaaactat tgaaaatctg ttatatatcg
    10381 atgctgatgt catctgtaaa ggctcattag ctggattgct tgatattaat tttgatgggg
    10441 ataagtttgc agctgttatt aaagatgtgc cttttatgca ggaaaaacca gcgaagcgtc
    10501 tggctataga gggacttcca gggaattatt tcaacgccgg tgtagtatat ctgcagcttg
    10561 aagcatgggc gaaaaatgat tttatgaata aagccattgc tatgctggca agtgacccgc
    10621 agcacacgaa gtataaatgc cttgatcagg atattttaaa tattctgttc tttggtcatt
    10681 gtatttttat tagcggcgat tatgattgct tttatggcat cgattatgag ttaaaaaata
    10741 aaagcgatga agattataaa aagaccatta ccgatgatac taagctgatt cattatgttg
    10801 gcgtaacgaa gccctggaac gactggacga attatccctg ccagaagtat tttaatgagg
    10861 cttatcaggc ttcttgctgg aatgatgtgg cgtttattcc agccacgaat gaaaagcagt
    10921 atcaagtg
//
LOCUS       DAJTZL010000137        10839 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.137, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000137 DAJTZL010000000
VERSION     DAJTZL010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10839
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.137"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(55..1338)
                     /gene="aroA"
                     /locus_tag="OO544_003116"
     CDS             complement(55..1338)
                     /gene="aroA"
                     /locus_tag="OO544_003116"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HCR6768645.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            complement(1409..2497)
                     /gene="serC"
                     /locus_tag="OO544_003117"
     CDS             complement(1409..2497)
                     /gene="serC"
                     /locus_tag="OO544_003117"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HCR6768646.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            complement(2696..3388)
                     /locus_tag="OO544_003118"
     CDS             complement(2696..3388)
                     /locus_tag="OO544_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HCR6768647.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKPGLLILPSDCTQRYKVVPESADYACIRCSEIIHMKAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            3518..5278
                     /locus_tag="OO544_003119"
     CDS             3518..5278
                     /locus_tag="OO544_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HCR6768648.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            5684..6541
                     /gene="focA"
                     /locus_tag="OO544_003120"
     CDS             5684..6541
                     /gene="focA"
                     /locus_tag="OO544_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HCR6768649.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            6596..8878
                     /gene="pflB"
                     /locus_tag="OO544_003121"
     CDS             6596..8878
                     /gene="pflB"
                     /locus_tag="OO544_003121"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCR6768650.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKQLEKIVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKLAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDVLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            9070..9810
                     /gene="pflA"
                     /locus_tag="OO544_003122"
     CDS             9070..9810
                     /gene="pflA"
                     /locus_tag="OO544_003122"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HCR6768651.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(9903..10079)
                     /locus_tag="OO544_003123"
     CDS             complement(9903..10079)
                     /locus_tag="OO544_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005043118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HCR6768652.1"
                     /translation="MQSERIYLVWAHPRHDSLTAHIADAIHQRAMERKIQVTELDLYR
                     RNIISRYYLRYGIW"
     gene            10179..>10839
                     /locus_tag="OO544_003124"
     CDS             10179..>10839
                     /locus_tag="OO544_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6768653.1"
                     /translation="MRMNMSDFATFFAVARNQSFRAAGDELGLSSSAISHSIKTLEQR
                     LKIRLFNRTTRSVSLTEAGSNLYERLRPAFDEIQIMLDEMNDFRLTPTGTLKINAARV
                     AARIFLMPLLVGFTREYPDIKVELTTDDSLVDIVQQGFDAGVRLSGIVEKDMISVAIG
                     PPVKLCVAATPEYFARYGKPRHPHDLLNHQCVVFRYPSGKPFHWQFAKEQEIAVAGNI
                     IL"
BASE COUNT         2790 a         2711 c         2568 g         2770 t
ORIGIN      
        1 atgtgccgac gttttgataa agaaagattt ggctatttat tgcccgttgt tcattcaggc
       61 tgcctggcta atccgcgcca actgctcgaa ataatccgga aatgttttgg ccgtacattt
      121 gggatcaaga atcgtcactg gtgtatctga caacgccacc agcgagaaac acatcgccat
      181 ccggtgatca ttgtatgtcg cgatctcggc aaagttcagt ttttccggtg gagtgatacg
      241 aatgtaatcg tgcccctctt ctacttccgc accgacttta cgcagttctg ttgccatcgc
      301 aaacaggcga tcggtctctt taacacgcca gttatagata ttgcgcagcg tggtggtgcc
      361 ttttgcaaat aacgccgccg tggcaatggt catcgccgca tcgggaatat ggttcatatc
      421 catatcaata gcgttcagtt caccacgcgt gcaggaaata taatcatcgc cccagcaaat
      481 ggtcgcgccc attttttcca gcacatcagc aaagcgaata tcaccctgca tactgttacg
      541 tccaataccg gtcactttta cagtgccgcc tttgattgct gctgctgcca gaaagtaaga
      601 agccgaagat gcatcgcctt cgaccaaata agtacccgga gactgataag actgcccgcc
      661 ttttacgaca aattgttgat agtgctgatt ttcaatttca acaccaaacg tcttcatcag
      721 attgagtgtg atgtcgatat aaggtttaga aaccagatcg cctttaatac gaatcaccgt
      781 atcttccggc gcaagaggcg cagtcattaa cagtgcggtg aggaattggc tggaaacgga
      841 gccatcaacg tcaacgttgc cgccagtaaa gccgccctgt aaacgcaacg gcggataatt
      901 ttcttgttcc aggtaagtga tcttcgcccc gcccaggcgc agcgcatcca ccagatgacc
      961 aatcgggcgt tctttcatac gcggctcacc ggtcagcaca atatcattgc tacccagaca
     1021 aagagctgcc gccagcggac gcattgccgt tccggcgtta ccgaggaaca actccagggc
     1081 accttctgcg tgtaatggac cgccgttacc gataatttcg caacgcgtac gatcggctga
     1141 aagcgtatag cttaccccta acgctgttaa tgcattcagc atatggcgca cgtcatcgct
     1201 atccagcaga ttggttaata ctgttttgcc gtgtgctaat gccgccagca ataaagcgcg
     1261 gttagaaacg ctcttggaac cgggcagatt aatagtgcca tcgacacgag cgatgggttg
     1321 taacgtcagg gattccatga actcaactct ctacaacaga aataaaaacc ccacaggctg
     1381 gctgtgggga ttaagcaaaa tttcggcatt aaccgtgacg gcgttcgaac tcaaccatga
     1441 agtctgtcag cgctttaacg ccttccagcg gcatggcgtt ataaatagaa gcgcgcattc
     1501 cgccgaccac acggtgacct ttcagtgcat gaaggccagc agcaaaagac tcttcaagga
     1561 acaatttgtc aagcgcactg tccgccaact ggaacggcac gttcatccgc gaacggttag
     1621 ctttcgccac gtcattgcgg tagaaatcgc tgttatcaat caccccatat agcagttctg
     1681 ctttttgctg attgatttta tccatttcag ctacaccgcc gttcgctttc agccatttaa
     1741 agaccagacc agatagatac caggcaaatg tcggcggcgt gttaaacatg gagccgttat
     1801 cgttgaggat ggaataatcc agaatcgacg gacacgcgat attcgctttg cccagcaaat
     1861 cttcacgaac gatgacgatt gtcaggccag ccgggccgat atttttctgc gcgccagcgt
     1921 aaatcacgcc ataacggctg acgtcaatcg gacgggaaag aatggttgaa gagaagtcgg
     1981 cggcgaccac cacatctttg ccgaagtctg gcgtttcgtc gatggcgata ccgtcgatgg
     2041 tttcattcgg gcagtaatgc atataagcag cattatcaga gagttgccat tcacgcattg
     2101 gcttaaccgc gcgcagacca tcaacagtca ctttggcgtc aaagacatta ggcgtgcagt
     2161 attttttcgc ttctttaatg gcacttgccg cccagtaacc ggcatcaaca taatctgcgg
     2221 tggttttatc accgagaata ttcagcggta ccgcagcaaa ctgaccgcga ccaccgccat
     2281 ggcagaataa taccttgtag ttggagggga cattaagaag atcgcgaaaa tccttctcgg
     2341 cttcctctgc aacctgaatg aactctttgc cacggtgact cacttccatc accgacgtac
     2401 caagaccgtt ccagtcgcgc agttcctgtt gagcctgttt aagcacctct gccggtagca
     2461 ttgccggacc agaactaaaa ttgaagattt gagccattgc ccctcaccac gttgcgttgc
     2521 tattaacccg cggtcaatcg attgcgacca caggtaatac attattcctg tggctatcgg
     2581 ttttatcatt cagtgacaca catcgcaatg agtaaaaccg ttgagggcaa aaatgtggct
     2641 tgcatcacac aaaagaatct ctcggattat tagcaaaaag ccgatgtttt tactgtcagg
     2701 tcacccgttt tgcccgactt gccttcgtcc attctggatt tgcacagcgc ggacataatt
     2761 gtttttcccc cgctttcata tgaatgattt cactacaacg aatacaggca tagtccgccg
     2821 actccggcac cactttgtaa cgctgcgtac aatcactggg taaaataagt aaacccggtt
     2881 tcaccttgtc atcttcaaag aaatagacac tgatttgccc gttggtttcg agaatcgcca
     2941 gacgtacctg ccccagctgc tccacgccac gcaatcgtag ctccataaag aactcaaatt
     3001 ccgtcatgtt ggagttattg agtttcgacc aggccagctc gccatcttca ataataacaa
     3061 ccggcttacc ttccagtaga tcttccagtt tttcactgtg cgccatcaac cacattacca
     3121 ggcggtataa caacgccaga gtgataaaga cgataagtac cggcaccatc ggcacatcat
     3181 cataaaacgc cacatctccc gccgctgaac ccagcgtgag aatgattaac acttcaaaca
     3241 gcgacatctg tcgcacaccg cgtcttccgg tcattttgag gaacaaaaaa accagtacaa
     3301 aggtataaag actacgtagt gcaacttcgc caaggaaatc aaaaggcact ttatcaaatg
     3361 ccatacggtg gagatcgaac gccttcattt ttatgctctt atcgtggttt atccgctaaa
     3421 agcatagtca acagttcatc ggcatggcgg cggtgatagc gcctttgaga taaaacccgt
     3481 attattgcgc gctttccgta cgactaaagt gattttcatg acgcaaacat ttatccccgg
     3541 caaagatgcc gctctggaag attccatcgc tcgcttccag caaaaacttt cagacctcgg
     3601 ctttcagatt gaagaggcct cctggctgaa tcctgtgcct aacgtctggt ctgtacatat
     3661 tcgcgacaaa gagtgcgcac tgtgttttac caacggtaaa ggcgcaacca aaaaagcggc
     3721 gctggcttct gcacttggtg aatatttcga gcgtctctca accaactact tttttgctga
     3781 tttctggctg ggcgaaacca tcgccaacgg tccgttcgtg cattatccca acgaaaaatg
     3841 gttcccactg accgaaaatg acgatgtgcc agaaggacta ctcgatgacc gtctgcgcgc
     3901 gttttatgat ccggagaatg aactgaccgg cagcatgctg attgacctac aatccggtaa
     3961 cgaagatcgt ggtatttgcg gcctgccgtt tacgcgtcag tccgacaatc agaccgttta
     4021 tattccgatg aatatcattg gtaacctgta cgtctccaac ggtatgtccg caggtaatac
     4081 ccgcaacgaa gcacgcgttc agggattgtc tgaagttttc gaacgctacg tgaaaaaccg
     4141 cattattgct gaaagcatca gcctgccaga gatcccggca gacgtgctgg cgcgttaccc
     4201 agcagtagtt gaagccatcg aaacgctgga agcggagggt ttcccaatct tcgcttatga
     4261 cggttcgctt ggcggccagt atccggtgat ttgcgtggta ctgttcaatc ctgctaacgg
     4321 tacctgcttt gcctctttcg gtgcgcatcc tgattttggc gtagcactgg aacgtaccgt
     4381 gaccgagctg ctgcaaggtc gtggcctgaa agatttggat gtgtttactc cgccaacctt
     4441 cgatgatgaa gaagtcgctg aacataccaa cctcgaaacg cactttatcg attccagcgg
     4501 tttaatctcc tgggacctgt tcaagcagga tgccgattat ccgtttgtgg actggaattt
     4561 ctccggcacc acggaagaag agtttgctac gctgatggct atcttcaaca aagaagataa
     4621 agaagtttat attgccgatt acgagcatct gggcgtttat gcttgccgta ttatcgtgcc
     4681 tggcatgtcc gatatttatc cggctgaaga tctgtggctc gcgaataaca gtatgggcag
     4741 ccatttacgt gaaacgattc tttcgctacc aggcagcgag tgggaaaaag aagattacct
     4801 gaacctcatc gagcaactgg atgaagaagg ttttgatgac tttacccgcg tgcgtgagct
     4861 gttgggtctg gcgaccgggt cggataacgg ttggtacacc ctgcgtatcg gtgaattaaa
     4921 agccatgctg gcgctggctg gtggcgatct ggaacaggct ctggtctgga ccgaatggac
     4981 gatggagttt aactcatcag tatttagtcc ggaacgcgcc aactattatc gctgcctgca
     5041 aacgttgtta ttactggcac aggaagaaga tcgccagccg ctgcaatatc tgaatgcgtt
     5101 tgttcgcatg tacggcgcag atgccgtaga agccgccagt gcggcaatga gcggcgaagc
     5161 ggcgttttac ggcctgcaac cggtagatag cgatctgcac gcgtttgctg cacatcagtc
     5221 gttgttgaag gcctacgaaa agctgcagcg cgccaaagca gcattctggg caaaataaaa
     5281 tcaaatagcc tacgcaatgt aggcttaatg attagtctga gttatattac ggggcgtttt
     5341 tttaatgccc cgctttacat atatttgcat taataaaata attgtaatta taaggttaaa
     5401 tatcggtaat ttgtatttaa taaatacgat cgatattgtt actttattcg cctgatgctc
     5461 ccttttaatt aactgtttta gcggaggatg cggaaaaaat tcaactcatt tgttaatttt
     5521 taaaatttat ttttatttgg ataatcaaat gtttactccg tatctgcata aaaaccatgc
     5581 gagttacggg cctataagcc aggcgagata tgatctatat caatttctca tctataatgc
     5641 tttgttagta tctcgtcgcc gacttaataa agagagagtt agtgtgaaag ctgacaaccc
     5701 ttttgatctt ttacttcctg ctgcaatggc caaagtggcc gaagaggcgg gtgtctataa
     5761 agcaacgaaa catccgctta agactttcta tctggcgatt accgccggtg ttttcatctc
     5821 aatcgcattc gtcttctata tcacagcaac cactggcaca ggcacaatgc ccttcggcat
     5881 ggcaaaactg gttggcggca tttgcttctc tctggggctg attctttgtg ttgtctgcgg
     5941 agccgatctc tttacttcca ccgtgttgat tgttgttgct aaggcgagtg ggcgcatcac
     6001 ctggggtcag ttggcgaaaa actggctaaa tgtctatttt ggcaacctgg tcggcgcact
     6061 gctgtttgta cttttaatgt ggctttccgg cgagtatatg accgcaaatg gtcaatgggg
     6121 actaaacgtc ctacaaaccg ccgaccacaa agtgcaccat acttttattg aggccgtctg
     6181 tcttggtatc ctggcaaacc tgatggtatg tctggcagta tggatgagtt attctggccg
     6241 cagcctgatg gacaaagcgt tcattatggt gctgccggtc gcgatgtttg ttgccagcgg
     6301 ttttgagcac agtatcgcaa acatgtttat gatcccgatg ggtattgtaa tccgcgactt
     6361 cgcatccccg gaattttgga ccgcagtcgg ttctgcaccg gaaaattttt ctcacctgac
     6421 cgtgatgaat ttcatcactg ataacctgat tccggttacg atcggcaaca ttatcggtgg
     6481 tggtttgttg gttgggttga catactgggt catttacctg cgtgaaaacg accaccatta
     6541 atggttgtcg aagtacgcag taaataaaaa atccacttaa gaaggtaggt gttacatgtc
     6601 cgagcttaat gaaaagttag ccacagcctg ggaaggtttt accaaaggtg actggcagaa
     6661 tgaagtaaac gtccgtgact tcattcagaa aaactacact ccgtacgagg gtgacgagtc
     6721 cttcctggct ggcgctactg aagcgaccac caccctgtgg gacaaagtaa tggaaggcgt
     6781 taaactggaa aaccgcactc acgcgccagt tgactttgac accgctgttg cttccaccat
     6841 cacctctcac gacgctggct acatcaacaa gcagcttgag aaaatcgttg gtctgcagac
     6901 tgaagctccg ctgaaacgtg ctcttatccc gttcggtggt atcaaaatga tcgaaggttc
     6961 ctgcaaagcg tacaaccgcg aactggatcc gatgatcaaa aaaatcttca ctgaataccg
     7021 taaaactcac aaccagggcg tgttcgacgt ttacactccg gacatcctgc gttgccgtaa
     7081 atctggtgtt ctgaccggtc tgccagatgc atatggccgt ggccgtatca tcggtgacta
     7141 ccgtcgcgtt gcgctgtacg gtatcgacta cctgatgaaa gacaaactgg cacagttcac
     7201 ttctctgcag gctgatctgg aaaacggcgt aaacttggaa cagactatcc gtctgcgcga
     7261 agaaatcgct gaacagcacc gcgctctggg tcagatgaaa gaaatggctg cgaaatacgg
     7321 ctacgacatc tctggtccgg ctaccaacgc tcaggaagct atccagtgga cttacttcgg
     7381 ctacctggct gctgttaagt ctcagaacgg tgctgcaatg tccttcggtc gtacctccac
     7441 cttcctggat gtgtacatcg aacgtgacct gaaagctggc aagatcaccg aacaagaagc
     7501 gcaggaaatg gttgaccacc tggtcatgaa actgcgtatg gttcgcttcc tgcgtactcc
     7561 ggaatacgat gaactgttct ctggcgaccc gatctgggca accgaatcta tcggtggtat
     7621 gggcctcgac ggtcgtaccc tggttaccaa aaacagcttc cgtttcctga acaccctgta
     7681 caccatgggt ccgtctccgg aaccgaacat gaccattctg tggtctgaaa aactgccgct
     7741 gaacttcaag aaattcgccg ctaaagtgtc catcgacacc tcttctctgc agtatgagaa
     7801 cgatgacctg atgcgtccgg acttcaacaa cgatgactac gctattgctt gctgcgtaag
     7861 cccgatgatc gttggtaaac aaatgcagtt cttcggtgcg cgtgcaaacc tggcgaaaac
     7921 catgctgtac gcaatcaacg gcggcgttga cgaaaaactg aaaatgcagg ttggtccgaa
     7981 gtctgaaccg atcaaaggcg atgtcctgaa ctatgatgaa gtgatggagc gcatggatca
     8041 cttcatggac tggctggcta aacagtacat cactgcacta aacatcatcc actacatgca
     8101 cgacaagtac agctacgaag cctctctgat ggcgctgcac gaccgtgacg ttatccgcac
     8161 catggcgtgt ggtatcgctg gtctgtccgt tgctgctgac tccctgtctg caatcaaata
     8221 tgcgaaagtt aaaccgattc gtgacgaaga cggtctggct atcgacttcg aaatcgaagg
     8281 cgaatacccg cagtttggta acaacgatcc gcgtgtagat gacctggctg ttgacctggt
     8341 agaacgtttc atgaagaaaa ttcagaaact gcacacctac cgtgacgcta tcccgactca
     8401 gtctgttctg accatcactt ctaacgttgt gtatggtaag aaaacgggta acaccccaga
     8461 cggtcgtcgt gctggcgcgc cgttcggacc gggtgctaac ccgatgcacg gtcgtgacca
     8521 gaaaggtgca gtagcctctc tgacttccgt tgctaaactg ccgtttgctt acgctaaaga
     8581 tggtatctcc tacaccttct ctatcgttcc gaacgcactg ggtaaagacg acgaagttcg
     8641 taagaccaac ctggctggtc tgatggatgg ttacttccac cacgaagcat ccatcgaagg
     8701 tggtcagcac ctgaacgtta acgtgatgaa ccgtgaaatg ctgctcgacg cgatggaaaa
     8761 cccggaaaaa tatccgcagc tgaccatccg tgtatctggc tacgcagtac gtttcaactc
     8821 gctgactaaa gaacagcagc aggacgttat tactcgtacc ttcactcaat ctatgtaatt
     8881 agatttgact gaaatcgtac agtaaaaagc gtacaataaa ggctccacga aagtggggcc
     8941 ttttttagca cgagagcctt ttttgtcagc tatctatact ctaaggtgac tgccaaaaca
     9001 gactcgacgt agccttcgag ctgcgcacca acacggcctc agatgggcca catctggaga
     9061 aacaccgcaa tgtcagttat tggtcgcatt cactcctttg aatcctgtgg aaccgtagac
     9121 ggcccgggta ttcgctttat cacctttttc cagggctgcc tgatgcgctg cctgtattgt
     9181 cataaccgcg acacctggga tacgcatggc ggtaaagaag ttaccgttga agatttaatg
     9241 aaagaggtgg tgacctatcg ccactttatg aacgcttccg gcggcggcgt taccgcatcc
     9301 ggcggtgagg caatcctaca agctgagttt gttcgtgact ggttccgcgc ctgcaaaaaa
     9361 gaaggcattc atacctgtct ggacaccaac ggttttgttc gtcgttacga tccggtgatt
     9421 gatgaactgc tggaagtaac cgacctggta atgctcgatc tcaaacagat gaacgacgag
     9481 atccaccaaa atctggttgg agtttccaat caccgcacgc tggagttcgc caaatatctg
     9541 gcgaacaaaa atgtgaaggt gtggatccgc tacgttgttg tcccaggctg gtctgacgat
     9601 gacgattcag cgcatcgcct cggtgaattt acccgtgata tgggcaacgt tgagaaaatc
     9661 gagcttctcc cctaccacga gctgggcaaa cacaaatggg tggcaatggg tgaagagtac
     9721 aaactcgacg gtgttaaacc accgaagaaa gagaccatgg aacgcgtgaa aggcattctt
     9781 gagcagtacg gtcataaggt aatgttctaa tttccccgta aagcggcaac tttattgagt
     9841 tgccgctttt ttattccgca cttcattctg ctacagcgtt tttaacacat cttatagtgc
     9901 atctaccata tcccgtacct gagataataa cgactgataa tattacgccg atataaatcg
     9961 agttccgtca cctgtatttt ccgctccatt gcccgctgat ggatcgcatc agcaatatgt
    10021 gcggtcaatg aatcatgacg aggatgggcc cataccaaat aaatacgttc agactgcata
    10081 attttacctc cacgttaagt tctggagcgc actttacgca ggcggcataa taatgtaaac
    10141 gtgatgattc aggatgagtt aatgaaggat tttcactaat gcggatgaat atgtctgact
    10201 ttgccacttt ctttgctgtg gcccgtaatc aaagctttcg tgcagcgggc gatgagttag
    10261 gcttatcctc gtccgccatt agccatagta ttaaaacact ggaacaacgt cttaaaattc
    10321 gcttattcaa tcggacaacc cgaagcgtat cgttaacgga agcgggttct aatctctatg
    10381 aacgattgcg cccagctttc gacgaaattc agatcatgtt agatgagatg aatgattttc
    10441 gtctgacacc aacaggaacg ctcaaaatca atgccgcccg ggtagctgca cgtatatttt
    10501 taatgccttt actggttggt ttcacacgtg aatatcctga tattaaagtc gagctcacaa
    10561 ctgacgactc tcttgtcgat atcgtccaac agggctttga cgctggcgtt cgacttagtg
    10621 gcatcgtcga aaaagatatg atcagcgtgg cgatcggccc accagtgaaa ttatgtgtag
    10681 cagcaacgcc agagtacttt gcaagatatg gcaaaccccg ccatcctcat gatctgctta
    10741 atcatcaatg tgttgtattt cgttatccca gcggcaaacc tttccactgg caatttgcaa
    10801 aggagcagga aattgctgtc gcagggaata tcattctgg
//
LOCUS       DAJTZL010000138        10687 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.138, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000138 DAJTZL010000000
VERSION     DAJTZL010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10687
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.138"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..784)
                     /locus_tag="OO544_003125"
     CDS             complement(<1..784)
                     /locus_tag="OO544_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6768654.1"
                     /translation="MTRIACVGITVQDRIYSLPTLPEGGGKYQANHYLEIGGGPAATA
                     AVAIAKLGVEVDFIGRVGDDSCGNTLLAELEGWGVNTAFCRRYPNARSSQSAILVDQH
                     GERIIVNYPSPDLGTDAEWLEAIDFSRYDLILADVRWHSGTEKAFSLARLAGVTTLLD
                     ADMTPQDISPLVALADHAVFSTPGLKRMTGLQSPEEGLFQATTQTAGKVYVTLGSEGS
                     LWIEDGHLCQQEAFSVNVVDTTGAGDVFHGALAVALAEKMPTK"
     gene            957..1835
                     /gene="yihU"
                     /locus_tag="OO544_003126"
     CDS             957..1835
                     /gene="yihU"
                     /locus_tag="OO544_003126"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HCR6768655.1"
                     /translation="MATIAFLGLGQMGSPMANNLLQKGHSLQVFDVNAQAVDALVTQG
                     ATAAQTPAEAAAGAEFIITMLPNGDIVRQVLLGEKGVCEAVSSDALLIDMSTIHPLQT
                     DALIRELQEKGISMMDAPVGRTSVNAIDGTLLILAGGTDEQIARARPILMCMGNELVE
                     AGGPGMGIRVKLINNYMSIALNALSSEAAVLCESLGLNLDVAIKVMSGTAAGKGHFTT
                     TWPGKVLKGDLSPAFMVDLALKDLRIAVDVARETGAPLNMGIAAESYYAAASQDGKGR
                     QDWSALLNQVRQQAGL"
     gene            1860..2747
                     /locus_tag="OO544_003127"
     CDS             1860..2747
                     /locus_tag="OO544_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HCR6768656.1"
                     /translation="MSSHFTPFTLKDISRPGGGFAMLAVDQREAMRLMFAAAGQPKPI
                     ADSVLTDFKVAATRILSPYASAVLADKQFCLEQIVEQGAVANSCGLIVAADLFIPGNG
                     IPVDSVEIDMSVDPHKAREMGAKAMKLLVLWREDEPAEERLAMVDKFVRRCRSAGLVS
                     IIEPVVRPPRRGWDFDRESTIVAAAAELGGTEADLYKAEMPLGGKGDEKTLLAACQQL
                     NDQMKMPWVILSSGVDADIFGRAVSIAMKGGASGFLAGRAVWASVVGAQDPQTMLRDV
                     SVPRLQRLAEIVDEGIAQR"
     gene            2780..3790
                     /locus_tag="OO544_003128"
     CDS             2780..3790
                     /locus_tag="OO544_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HCR6768657.1"
                     /translation="MDKITQVLFSDIGKVTTQYVEVPHQELKPHEVRIAPVFYGICGS
                     DLHVLKGGHPFAKPPVVPGHEIAARVTEVGSDVKNVQPGDHVVVDPIMACMECRACKA
                     GRFNLCEPPQVAGFRAPGFARSQHIVPARNCHVAPASLPLKMLAFAEPAACARHCVNR
                     MPKASLESVLVIGAGTIGLSIVQALRIMGAGKITVIEPDAAKRALALKLGAAEVWAPG
                     ELAADVRFTGAIDVVAAQATLNDACTRVYAGGTVVCMGVPSGPREIPLPMMQRFERDL
                     LNSGMYIPEDFDAVIEWLADGRFDTSELVTDLFAIEDAAAAFERAQQNDSIKVMLQFA
                     PE"
     gene            3860..5293
                     /locus_tag="OO544_003129"
     CDS             3860..5293
                     /locus_tag="OO544_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6768658.1"
                     /translation="MSQITSPATYSISRPQDVIDIVNKNSAINTSIGVIFIALGGILI
                     DAYQAAMVGFGNKYIAAQFGISPGLAATVNASVLIAALIGGLLANRVINRFGQKRAFI
                     IGMGLCTIGAAAVAIAPSIWWVLVCRVIMGFGLGIDFPLATNAVAELRGSTSKKTGTS
                     VNLWQMAWYVSTTVVYLVLLPLLLSGIAEEQLWRYGIFIGAIFAVIFMILRYFFIGES
                     AMWAARVGRYQEACDILGKRYGVQARVAASGTTEAKFSEKAENKYSGGYGILFNDRYR
                     KRTILGCVVATMQAWQYNAVGVYLPLTLAGIISGGLTGALTGSAVVNALCGVTGGMIG
                     SFILQRLGTRRQSMYGFAVVTLALLSLGALATTNPWLSLGLLGSIIFFHSAGPGGLGM
                     TIATLSYPPAIRPTGVGFARAIMRTGAIAGLIFWPMLWGALKTEAFYWLAIVPFLGFL
                     TCVLINWEPLGANVDAEDAEVLAELKK"
     gene            5392..6741
                     /locus_tag="OO544_003130"
     CDS             5392..6741
                     /locus_tag="OO544_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HCR6768659.1"
                     /translation="MSEKLPAPREGLSGKAMRRVVMGSFAGALMEWYDFFIFGTAAGL
                     VFAPLFYPDSDPFIGLIAAFATFGVGFLTRPLGGIVFGHFGDKIGRKITLIWTLAIVG
                     CSTFLIGFIPTYQEIGIWAPLVLMVLRLIQGFGLGGEYGGAALMTIESAPESRRGFLG
                     SLPQTAASVGIMLATGIFALCNHFLTSEQFLSWGWRIPFWLSAVMLIVGLFIRLHTEE
                     TLDFQKQKTTNNKEKSVPPLIELFKKHPRNILLALGARLAESVSSNIINAFGIVYISS
                     QLALSRDIPLTGMLIASAIGIFSCPLVGWLSDRIGQKSLYLSGAGFCVLFAFPFFLLL
                     DSKSTLIIWCSMILGYNLGPTMMFAVQPTLFTRMFGTKVRYTGLSFAYQFSAILGGLS
                     PLIASSLLALGGGKPWYVALFLFAVSVLSFVCVWLIEPTDEQETASYRYIREQSHEN"
     gene            6731..7618
                     /locus_tag="OO544_003131"
     CDS             6731..7618
                     /locus_tag="OO544_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HCR6768660.1"
                     /translation="MKTERKNEHYLALQQAFDAPWPGPVGELVTLEKGNIHLQIYPHD
                     GARITSLKAFGSEVLRQWQPQRRAFQYGCFPMVPWAGRLGNATLNAGGQCYSLPANKP
                     PHALHGMACYSTWEIIDKTADSLTLRMPLASPWPWQGEVIQTFLLENDALVLQLEVHS
                     YADTFPASAGWHPWFAKKLTPQNTESLQVLFDADWQEEAGSDELPTGNRIFPQAGPWD
                     DCFGFYNGVKVKLLWPGKLAMTMISSANSLVVFDKQPDATCINPLTQAPNAINLTPEL
                     VTPDRPLVIETRWQFTPES"
     gene            7759..8358
                     /gene="yihX"
                     /locus_tag="OO544_003132"
     CDS             7759..8358
                     /gene="yihX"
                     /locus_tag="OO544_003132"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HCR6768661.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            8352..9224
                     /locus_tag="OO544_003133"
     CDS             8352..9224
                     /locus_tag="OO544_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HCR6768662.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLFSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            9221..9658
                     /gene="dtd"
                     /locus_tag="OO544_003134"
     CDS             9221..9658
                     /gene="dtd"
                     /locus_tag="OO544_003134"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HCR6768663.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGVSPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            9703..10644
                     /gene="fabY"
                     /locus_tag="OO544_003135"
                     /gene_synonym="yiiD"
     CDS             9703..10644
                     /gene="fabY"
                     /locus_tag="OO544_003135"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HCR6768664.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFINQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
BASE COUNT         2462 a         2532 c         2854 g         2839 t
ORIGIN      
        1 cttttgttgg cattttttct gccaacgcta ccgcgagtgc gccgtgaaaa acatcgcctg
       61 ctccagtggt atcaaccaca ttgacagaaa atgcctcctg ttgacacaaa tgaccatctt
      121 caatccacaa cgatccttca gaacccagcg taacatacac tttacctgct gtttgtgtcg
      181 ttgcctggaa caaaccttct tcaggtgact gcaggccagt catgcgtttc agccctggtg
      241 ttgagaacac ggcgtgatcg gcaagcgcga caagtggaga aatatcctgc ggtgtcatat
      301 cggcgtctaa aagcgttgtc acacccgcaa ggcgcgctag cgagaatgct ttttcagtac
      361 cgctatgcca gcgaacatcg gccagaatca gatcataacg agagaaatcg atcgcttcta
      421 accattcggc atcagtaccg agatcaggac ttggataatt aacgataatc cgttcgccat
      481 gctgatcaac gaggatcgct gattgcgacg aacgggcgtt aggatatcgg cggcaaaatg
      541 cggtattgac gccccaaccc tcaagttctg caagtaaggt atttccacaa ctgtcatcgc
      601 cgacgcgacc gatgaaatca acctccacgc caagctttgc gattgcaaca gcggctgttg
      661 ctgccgggcc gccaccaatc tcaagataat gatttgcctg atatttcccg ccgccctccg
      721 gcagagtggg caaagaataa attctgtcct gaactgtgat acccacgcat gcaattctgg
      781 tcatatttgt cttgtcctgt ttaattttgt cgtctttggt caatttaaat gacgcaaatg
      841 atcaaattag tgatgcgtgt cgtgtttttg aacataaatt acaaatatgc tcaaaaacag
      901 acgaaaacga attttcaatt gtcattattt tgtcaatgtc agacaggaga gtattcatgg
      961 caaccattgc atttctcggg ctggggcaga tgggtagccc aatggctaac aatctattgc
     1021 aaaaagggca ttcattgcag gtatttgatg tcaatgcaca ggccgtcgat gcgctagtta
     1081 cgcagggggc cactgcggca caaaccccag cggaagcagc agcaggcgct gaatttatca
     1141 tcactatgct tcccaatggt gacattgttc gacaggttct gttgggggaa aaaggcgtct
     1201 gtgaggctgt ttcatctgac gcgttattga ttgatatgtc gactattcat cccttacaga
     1261 ccgatgcatt aatacgggag ttacaagaaa aaggcatcag tatgatggac gctcccgtgg
     1321 gcagaacgtc cgtgaatgcg attgatggca cattgctgat tttagcgggt ggcactgatg
     1381 agcagattgc acgtgcacgc ccgattctca tgtgcatggg taatgagctg gtcgaggctg
     1441 gcggcccagg aatggggatc cgcgtgaagc taattaacaa ctatatgagt attgcgctta
     1501 atgcgctgtc ctccgaggcc gctgtgttat gtgagtcact gggattaaat cttgatgtcg
     1561 caattaaggt gatgagtggt acggcagcag ggaaaggaca ttttacgacc acctggcctg
     1621 gcaaagtgct gaaaggcgat ctttcacccg ccttcatggt ggatctggcg ttgaaagatc
     1681 tgcgtattgc agttgacgtc gctcgggaga cgggggcacc gctcaatatg gggattgcag
     1741 cagaaagtta ttacgctgca gcaagtcaag acggcaaagg gcggcaggac tggagtgccc
     1801 tgctaaatca ggttcgccag caggctggtc tttaatttta tctattaaca ggagtttcta
     1861 tgtcatccca cttcacccca tttacgctga aagatatctc ccgcccaggt ggtggatttg
     1921 ccatgttagc tgtcgatcag cgtgaggcca tgcgcttgat gtttgcggca gcagggcagc
     1981 caaaacccat cgctgacagt gtactcaccg attttaaagt tgccgcgacc cgaatccttt
     2041 ctccttatgc ttctgccgtt ctggcggata aacaattttg tcttgagcaa atagtcgagc
     2101 agggcgctgt tgctaattct tgcggcctga ttgtcgctgc cgatcttttt atcccaggta
     2161 atggcatccc ggttgatagc gttgaaatag atatgtccgt cgacccgcat aaagcccgtg
     2221 aaatgggggc taaggcaatg aaattacttg tactctggcg tgaagatgaa cccgcagagg
     2281 aacgtctggc aatggttgat aaatttgttc gtcgctgccg tagtgccggg ctggtgagca
     2341 ttattgaacc tgtggtgcgt ccacctcgtc gcggttggga ctttgatcgt gaaagcacca
     2401 ttgttgccgc cgccgcagag cttggtggaa cagaggctga tctttacaaa gccgagatgc
     2461 cattaggggg caaaggcgat gaaaaaacct tacttgccgc ctgtcagcaa cttaacgatc
     2521 agatgaagat gccgtgggta attctttctt ctggcgttga tgccgatatt tttggccgtg
     2581 cagtaagtat tgccatgaaa ggtggtgcgt cgggcttcct ggctggacga gcggtttggg
     2641 cgtcggttgt tggtgcacaa gatccacaaa ctatgctgcg cgatgtctct gttcctcgtt
     2701 tacaacggtt ggcagaaatt gtcgatgaag gtatcgcgca acgctaatcc cctatggata
     2761 tcgattctgg agaataacaa tggataaaat tacccaggtt ttattttcag atattgggaa
     2821 agtcaccact caatatgttg aagtaccaca ccaggaactt aaaccgcacg aagtgcggat
     2881 tgcgcctgtg ttctatggga tatgcggttc ggatctgcat gttctgaaag gcggtcatcc
     2941 gtttgccaaa ccacctgtcg tccccggtca tgaaattgca gcgcgcgtta cggaagttgg
     3001 cagcgacgtt aaaaatgtac agccgggcga tcatgttgtg gtcgatccca tcatggcttg
     3061 catggaatgc cgagcctgca aagcaggacg ttttaatctt tgtgaaccac cgcaggttgc
     3121 tggttttcgc gcaccgggct ttgctcgctc acaacacatt gttcctgcgc gtaattgcca
     3181 tgtcgcacca gcctctttac cgctaaaaat gttggccttt gccgaaccgg cggcttgtgc
     3241 ccgtcactgc gttaaccgaa tgccgaaagc ttctctggaa agcgtactgg taattggtgc
     3301 cggaacgata ggcttatcca tcgtgcaggc actgcgcatt atgggggcag gtaagattac
     3361 cgtgattgaa cctgacgctg ccaaacgcgc gctggcgtta aagctgggcg cagcagaagt
     3421 ttgggcacca ggtgagctgg ccgcagatgt gcgatttacg ggggcgattg atgtcgttgc
     3481 agcgcaggcc acgcttaacg atgcatgtac ccgtgtatat gccggaggca ccgtcgtgtg
     3541 catgggcgta ccaagtgggc cgcgtgaaat accattaccg atgatgcaac gtttcgagcg
     3601 tgacttgctc aactctggca tgtacatccc tgaagatttc gatgctgtta tcgaatggct
     3661 ggcggatggg cggtttgata ccagtgaact ggttaccgat ttatttgcca ttgaggatgc
     3721 agcggcggca tttgaacgcg cgcagcaaaa tgactccata aaggtcatgc tgcaatttgc
     3781 gccggaatga ttttctaaat ctctgtctgg cgctgcgtca cgtcagcgcc accctacaac
     3841 aattaaaaag gacactgcga tgagtcaaat caccagtcct gcaacttatt caattagtcg
     3901 gccacaggac gtaattgata ttgttaataa gaactctgca atcaacacca gtatcggcgt
     3961 tatttttatt gctttgggcg gcatattgat tgatgcctac caggcggcga tggtggggtt
     4021 tggtaataaa tacattgccg ctcaattcgg catttcgccg ggtcttgctg caaccgtcaa
     4081 tgcatcagta ttaatcgccg cgttaattgg cggtttatta gcgaaccgag taataaaccg
     4141 ctttgggcaa aagcgggcat ttattattgg catggggctg tgcaccatcg gtgctgcagc
     4201 ggtagctatt gctcccagta tctggtgggt gctggtgtgc cgcgtcatca tgggctttgg
     4261 tttaggcatc gacttccctt tggcaaccaa tgccgtggca gagcttcgtg gttcaacgtc
     4321 gaagaaaacc ggaacgtcgg tcaacctctg gcaaatggcc tggtatgttt cgacaaccgt
     4381 tgtttatttg gtacttttgc cgctgcttct gtcgggtatc gctgaagaac aattgtggcg
     4441 ttacggaata ttcatcggag ctatttttgc agtcatcttc atgattttgc gttacttctt
     4501 tattggtgaa tccgcaatgt gggccgcacg cgtcgggcgt taccaggaag cgtgcgacat
     4561 tctgggaaaa cgttatggtg ttcaggctcg cgttgcggca tcgggtacaa cagaagcgaa
     4621 attctcggaa aaagcagaga ataaatacag tggtggatat ggcatcttat ttaatgatcg
     4681 ttaccgcaaa cgcaccattc ttggctgtgt cgtggcaacc atgcaggcgt ggcaatataa
     4741 cgccgtaggt gtttatcttc ctcttacgtt ggcgggaata ataagtggcg ggcttactgg
     4801 cgcgttgacc ggttctgccg tcgtgaatgc cctttgtggg gtgacaggcg ggatgatcgg
     4861 ctcgtttatt ctccaacgac tgggtactcg ccgacagtcg atgtatggat ttgctgttgt
     4921 gaccttagca ttgctgtcgt taggcgcact ggcaacgact aatccctggc tgtctttagg
     4981 gttattggga tcaattattt tcttccattc agcgggtcct ggtgggctgg gcatgaccat
     5041 tgccacactc tcttatcctc ccgctattcg ccctactggg gtcggatttg cccgcgctat
     5101 tatgcgcaca ggggcaattg caggactcat tttctggccg atgctgtggg gtgcgttgaa
     5161 aactgaagcg ttttactggt tggcaatcgt gccattcctg gggttcctga cctgcgtatt
     5221 gattaattgg gaaccactgg gtgcaaatgt tgatgctgag gatgcagagg ttctggctga
     5281 attgaagaaa taaattaatg cgcatcggct taaaccgatg cgcattaatt caaactgccc
     5341 acgtttagtg gttcgtatcc cagatttctc gcctaccagg agaggaaaat tatgtctgaa
     5401 aagttacccg caccgcgtga agggctttcc ggtaaagcca tgagacgtgt cgttatgggc
     5461 agtttcgcag gcgcgttaat ggaatggtat gacttcttta tttttggcac ggcagcagga
     5521 ctggtatttg caccgctgtt ttatccagac agcgatcctt ttataggtct tatagccgcc
     5581 tttgcgacct ttggtgtcgg ttttctgact cgtcctttag gtggcatcgt ctttggtcat
     5641 tttggcgata aaatcgggcg aaaaataaca cttatctgga cgctggctat tgtcggttgc
     5701 tccacatttt tgattggttt tattccgacg tatcaggaaa ttggcatttg ggcaccgctt
     5761 gtattaatgg tgctgcgttt gattcagggc tttggtcttg gtggcgagta cggcggagcg
     5821 gcattgatga ccatagaatc agcgcccgaa tcccgacgcg gatttttagg ttcactcccg
     5881 caaacggctg cgtctgtggg tattatgctg gcaaccggta tttttgctct ctgtaaccat
     5941 tttcttactt cggagcagtt tctctcctgg ggatggcgca ttccgttttg gctttctgcg
     6001 gtgatgttga tcgttgggct gtttatccgc ctgcataccg aagaaacact ggattttcaa
     6061 aagcaaaaaa cgacgaataa taaagaaaag tccgttcctc cgttgattga attattcaaa
     6121 aaacatccac gaaatatttt attggcactg ggtgcgaggc tggcggaaag tgtctcctct
     6181 aatattatta acgcctttgg tattgtctat atttccagcc aattagcatt gtcgcgagat
     6241 attcccctga cgggtatgtt gattgcctcg gcgatcggta ttttcagttg tccattggtt
     6301 ggatggttat cagatcgtat aggtcaaaaa agtctgtatt tgtcgggagc aggattttgt
     6361 gtcctgttcg cgtttccttt cttcttatta ctggatagca aaagtacgct catcatctgg
     6421 tgcagtatga tcctcggcta caacttaggt ccgacgatga tgtttgccgt gcagccaaca
     6481 cttttcaccc gcatgttcgg taccaaagta cgatataccg gcctttcttt tgcttatcaa
     6541 ttttccgcga ttcttggcgg actaagcccc cttatcgcct caagtttgct ggcgctaggg
     6601 ggaggaaaac cgtggtatgt cgctctgttt ttatttgctg tctcagttct ttcctttgtt
     6661 tgcgtctggc tgattgagcc gaccgatgaa caagaaaccg cttcttaccg ctacatcagg
     6721 gaacaatctc atgaaaactg aacgaaaaaa cgagcattac ctggcgctac aacaggcgtt
     6781 tgacgcgcca tggcctgggc ctgtaggaga gttagtgacg ctggaaaaag gcaatattca
     6841 cctgcagatt tatccgcatg atggtgccag gataacctcg ttaaaggcat ttggttctga
     6901 agtattacgc caatggcagc cgcagcgcag agcttttcag tatggctgtt tcccaatggt
     6961 gccgtgggca gggcgactgg ggaatgcgac gctgaatgct ggtgggcaat gttattcttt
     7021 acccgccaat aaaccaccac atgcgttgca tggtatggct tgttactcaa cgtgggaaat
     7081 catcgacaaa acagcagatt ccctgacttt acgtatgccc ctggcttcac cgtggccctg
     7141 gcaaggggaa gtgattcaga cttttttgct ggagaacgat gcgctggttt tgcaactgga
     7201 agtccattca tatgccgata cattcccggc ctccgcaggc tggcatccgt ggtttgcgaa
     7261 aaagctgact ccgcaaaata cagaatcgtt gcaggtgctt tttgatgcgg actggcaaga
     7321 agaagcgggt agtgatgaac tgccaactgg aaatcgtatc tttcctcaag ccggtccctg
     7381 ggatgactgt tttggttttt ataacggggt gaaggttaag ttactgtggc ctggtaaact
     7441 cgcgatgacc atgatctctt ctgcaaacag tctggtggta tttgataaac agcccgatgc
     7501 cacctgtatt aacccgctga ctcaggcacc taacgcaata aatctaacgc cggaattggt
     7561 gacaccagat aggccgctgg tcatcgaaac tcgttggcaa tttacacctg aatcttaacc
     7621 attcatctat tgcgaaaaag ccagtgaaat tactctgctt tttcgcaatc agcatcacaa
     7681 gattaccctt attgccacat agccaaaccc atcctttccc gctacagtta atttcttgtg
     7741 gcgcgaaagg aggcaaaaat gctctatatc tttgatttag gtaatgtgat tgtcgatatc
     7801 gactttaacc gtgtgctggg agcctggagc gatttaacgc gtattccgct ggcatcgctt
     7861 aagaagagtt ttcatatggg ggaggcgttt catcagcatg agcgtgggga aattagcgac
     7921 gaagcgttcg cagaggcgct gtgtcatgag atggctctac cgctaagcta cgagcagttc
     7981 tctcacggct ggcaggcggt gtttgttgcg ctgcgcccgg aagtgatcgc catcatgcat
     8041 aaactgcgtg agcaggggca tcgcgtggtg gtgctttcca ataccaaccg cctgcatacc
     8101 accttctggc cggaagaata cccggaaatt cgtgatgctg ctgaccatat ctatctgtcg
     8161 caagatctgg ggatgcgcaa acctgaagca cgaatttacc agcatgtttt gcaggcggaa
     8221 ggtttttcac ccagcgatac ggtctttttc gacgataacg ccgataatat agaaggagcc
     8281 aatcagctgg gcattaccag cattctggtg aaagataaaa ccaccatccc ggactatttc
     8341 gcgaaggtgt tatgctaaaa accattcagg acaaagccag gcaccgtacc cgtccactat
     8401 gggcctggct aaaactactc tggcaacgta ttgatgagga caacatgaca accctggcag
     8461 gtaaccttgc ctatgtgtct ttgctctcat tagtgccgct ggttgccgtt gtttttgcgc
     8521 ttttcgccgc ttttcccatg ttttccgacg tcagcattca gttacgtcac tttatttttg
     8581 ccaactttct gcctgctact ggcgatgtta ttcagcggta tatcgaacaa tttgttgcca
     8641 attccaacaa gatgaccgcc gttggggcgt gcgggctgat cgtcaccgcg ttattgttga
     8701 tgtactccat cgatagcgcg ttgaatacca tctggcgcag taaacgagcg cgaccaaaaa
     8761 tttactcgtt tgccgtgtac tggatgatct taacgctggg gccgctgctg gcaggggcca
     8821 gtctggcgat cagttcctat ttgttctccc tgcgctgggc gagcgatctc aatactgtca
     8881 tcgataacgt gctgcgtatt tttccgctgc tgttgtcgtg gatctccttc tggttgctgt
     8941 acagcattgt tcctaccatc cgcgtaccta accgcgacgc gattgtcggc gcgtttgtcg
     9001 ctgcactcct gttcgaagca ggaaagaaag gtttcgcgct ttatatcacc atgttcccgt
     9061 catatcagct catttacggc gtgctggcgg tgatccccat tctcttcgtt tgggtctact
     9121 ggacatggtg tatcgtcttg cttggcgcgg aaattactgt cactctcggg gaataccgca
     9181 aactaaaaca agcagctgaa caagaagaag acgacgaacc atgattgcat taattcaacg
     9241 cgtaacccgt gccagcgtca ccgtggaggg agaagtgacg ggcgaaattg gcgcgggact
     9301 tttggtgtta ttgggtgtcg aaaaggatga cgacgaacag aaagcaaacc gtctgtgcga
     9361 gcgtgtgctc ggctaccgca tctttagcga tgccgaaggc aagatgaatc tcaacgtgca
     9421 acaggcgggc ggcagtgtac tggtggtttc ccagtttacc ctcgccgcag ataccgaacg
     9481 ggggatgcgc ccaagtttct ccaaaggcgt atcaccggat cgcgcagagg cgttatatga
     9541 ctatttcgtc gaacgctgcc gccagcaaga gatgaacacg caaacaggac gcttcgctgc
     9601 cgatatgcag gtatcgctgg tcaatgatgg ccccgtgaca ttctggttgc aggtatgagc
     9661 ctgcttccag ggttgtcacg ggaaacaaga gagagtatcg ctatgtatca ccttcgggtt
     9721 ccacaaacag aagaagagtt agagcgttac tatcagtttc gctgggaaat gttgcgtaag
     9781 cccctgcatc aaccaaaagg ttcggaacgc gacgcgtggg atgcgatggc gcatcaccag
     9841 atggtcgtcg acgagcaggg taatctggtg gcggtaggcc gattgtatat taatgccgac
     9901 aatgaagcgt ccattcgctt tatggccgtt cacccggacg tgcaggacaa agggttagga
     9961 acgctgatgg cgatgaccct ggagtcggtg gcgcgtcagg aaggcgttaa gcgcgtgacc
    10021 tgtagcgccc gtgaagacgc ggtggagttt ttcgccaagc tgggattcat taatcagggg
    10081 gagatcacca ccccaactac cacgccgatt cgccattttt tgatgattaa acccgttgcc
    10141 actctggatg acattctgca tcgcggtgac tggtgcgcgc agctgcaaca ggcgtggtac
    10201 gaacatatcc cgttaagcga aaaaatgggc gtgcgcattc agcaatatac cgggcaaaaa
    10261 tttatcacca ccatgccaga aaccggtaat cagaatccgc accatacgtt gtttgccggg
    10321 agtttattct cgctggcgac gctcaccggt tggggactta tctggctgat gctgcgtgaa
    10381 cgccacctcg gcggaacgat tattctggcg gatgcgcata tccgctacag caagccgatt
    10441 agcggtaaac ctcatgcggt agccgacctt ggtgccttaa gcggcgatct cgaccgtctg
    10501 gcgcgcggac gaaaagcacg ggtgcagatg caagttgaaa tctttggcga cgagacgccg
    10561 ggtgcagtgt ttgaaggcac gtatatcgtt ctgcccgcga agccatttgg cccgtatgaa
    10621 gagggcggga acgaagaaga gtagcttatg gagaggggcg gtgctgcctc tctcattcat
    10681 tccggtc
//
LOCUS       DAJTZL010000139        10591 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.139, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000139 DAJTZL010000000
VERSION     DAJTZL010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10591
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.139"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(136..1164)
                     /gene="malI"
                     /locus_tag="OO544_003136"
     CDS             complement(136..1164)
                     /gene="malI"
                     /locus_tag="OO544_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HCR6768665.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGTTLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            1339..2931
                     /gene="malX"
                     /locus_tag="OO544_003137"
     CDS             1339..2931
                     /gene="malX"
                     /locus_tag="OO544_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HCR6768666.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIEGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            2941..4113
                     /gene="malY"
                     /locus_tag="OO544_003138"
     CDS             2941..4113
                     /gene="malY"
                     /locus_tag="OO544_003138"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HCR6768667.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHDVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            4217..5218
                     /gene="add"
                     /locus_tag="OO544_003139"
     CDS             4217..5218
                     /gene="add"
                     /locus_tag="OO544_003139"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HCR6768668.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLHYVELRFSPG
                     YMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(5254..6294)
                     /locus_tag="OO544_003140"
     CDS             complement(5254..6294)
                     /locus_tag="OO544_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR6768669.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSCVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            6538..6663
                     /gene="blr"
                     /locus_tag="OO544_003141"
     CDS             6538..6663
                     /gene="blr"
                     /locus_tag="OO544_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HCR6768670.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            6928..7143
                     /gene="ydgT"
                     /locus_tag="OO544_003142"
     CDS             6928..7143
                     /gene="ydgT"
                     /locus_tag="OO544_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HCR6768671.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            7229..7669
                     /locus_tag="OO544_003143"
     CDS             7229..7669
                     /locus_tag="OO544_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HCR6768672.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            7746..8327
                     /gene="rsxA"
                     /locus_tag="OO544_003144"
     CDS             7746..8327
                     /gene="rsxA"
                     /locus_tag="OO544_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HCR6768673.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFNGLVKL"
     gene            8327..8905
                     /gene="rsxB"
                     /locus_tag="OO544_003145"
     CDS             8327..8905
                     /gene="rsxB"
                     /locus_tag="OO544_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HCR6768674.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            8898..>10591
                     /gene="rsxC"
                     /locus_tag="OO544_003146"
     CDS             8898..>10591
                     /gene="rsxC"
                     /locus_tag="OO544_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HCR6768675.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRTRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVADPRKTAVEAAIAR"
BASE COUNT         2428 a         2736 c         2704 g         2723 t
ORIGIN      
        1 tctcaagttg tcagaattaa acacataaac ctcctgtgaa cttcagtaaa aataaaaggc
       61 accgcctgtg tgccacattt tttatcatta accttgagga aaggacacta ccaggtatag
      121 cagataaaga gaggattatt tcgctgcaat gagccgggcg ggaataataa gattgcgtga
      181 atgcgtctct tcatgggtga ttttttgcat catgcgatcc gcaagtgtgg taccaagttc
      241 ccgcgcaggc gtgctggccc aggtaacggg tatatcatca agtgtggttg gtgtcgcatc
      301 ggtaaatgcc gccagcgaaa cttgttgctc aaagtaacga tcgactccgc tttccccgct
      361 ttgccgccct gctttcagca aaccaaacca tgcccccatc gcaatagttt cgttatagca
      421 aaccacagca ctgatggtcg ggttatgacg taaaagcgcc gtgatagctt ccgcggcttg
      481 cttctggctg gatgtgcact ccagcaccca atcgctgtga aacggcaggc caaattttag
      541 tagagttgca caatagcctc ccacccgttc tgcccgggtt aatgaggaac tttgccctcc
      601 cagccaggcg atccgctgat gcccattgcg aatgagatgc tccgtcaaca actgtgcagc
      661 ctgcatgttg tccgggcgaa ccgtatcaac atcatcaaga taactggcac gggaagcgaa
      721 aatcaccggg atagcttttt cttctgccat ccgtcgcagg tcatcgctgc ttcctgcagc
      781 cccggcaatt accacaccat cgacaccctg attcagtaac agtgaaaacc gctgtgccag
      841 ctgctcgccg tctttaccgc cgtgaagcaa aaaaaccatc cgtccctgcg cttccagagc
      901 ttccgtcaat ccggccgtca attcggcgta aaacggcgca gataaatcac ggacgatcaa
      961 accaatgacg ccgctttgcc cgccgcgcag cgccgacgcc tggcgattgc gcacaaatcc
     1021 cagctcttca atggcggcgt taacgcgttc tcctgtggcg gtagagattc gccctttgcc
     1081 actaagcacc agcgaaacgg tacttaccga cacgcccgca gccagcgcaa catcatgaat
     1141 ggttattttt ttggcggtag ccatgcagat gacctactcc ctgattatgt gacagataaa
     1201 acgttttacc ttttatttat cttatacccg ctattatcgt tgcgtaatgt gatttatgct
     1261 tcactaaaat ttgataaaac gttttatctt ctcgcgcaat ttactgaatc cagattgttc
     1321 tctacgagga gtcgttttat gacggcgaaa acagcaccga aagtcacgct gtgggagttc
     1381 ttccagcagt taggcaaaac cttcatgtta cccgtggcat tattgtcgtt ctgcggcatt
     1441 atgctcggca ttggtagttc tcttagtagc cacgatgtca tcaccctgat cccggtcctg
     1501 ggcaaccccg tgttgcaggc tatctttacc tggatgagta agattggctc gtttgctttt
     1561 agtttcctgc cagtcatgtt ctgtatcgcc atcccgctgg gtctggcacg cgaaaacaaa
     1621 ggcgtagcgg cattcgctgg cttcgtcggt tatgcggtaa tgaacctcgc ggtaaacttc
     1681 tggttgacca ataaaggcat tctgccaacc acggacgccg cggttctgaa agccaataac
     1741 atccagagca ttcttgggat ccagtcgatc gacaccggga tcctcggtgc ggtgatcgcc
     1801 ggtattatcg tctggatgct gcatgagcga ttccataata tccgcctgcc ggatgcgctt
     1861 gcattcttcg gcggtacgcg cttcgtacca atcatctcct cgctggtgat gggccttgtc
     1921 ggcctggtga ttccattagt ctggccgatt ttcgcgatgg gtattagcgg cttgggccat
     1981 atgataaaca gcgcgggtga tttcggaccg atgctgtttg gtaccggtga acgtctgctg
     2041 ttgccgtttg gtctgcatca cattctggtg gcattaattc gctttaccga cgcaggcggc
     2101 acgcaggaag tctgcggtca aaccgtcagc ggcgcattga ccatcttcca ggcgcaattg
     2161 agttgcccga ccactcacgg tttttctgaa agcgccacgc gtttcctttc gcaaggcaaa
     2221 atgcctgcgt ttctcggcgg tctgccaggt gcagcgttag cgatgtatca ctgcgcgcgc
     2281 ccggaaaatc gccataaaat tgaagggctg ctgatttctg gcctgatcgc ctgtgtcgtt
     2341 ggcggcacga ccgaaccgct ggaattcctg ttcctgttcg tggcgccagt tctgtatgtc
     2401 atccacgcgc tgttaaccgg cctcggcttc actgtcatgt ctgtgctcgg cgtcaccatc
     2461 ggtaataccg acggcaatat catcgacttc gtggtgttcg gtattttgca tggtctgtca
     2521 accaagtggt acatggtgcc agtggtggcg gcaatctggt ttgtcgttta ctacgtcatc
     2581 ttccgtttcg ctatcacccg cttcaatctg aaaaccccgg ggcgcgatag cgaagtggcc
     2641 agttcaatcg aaaaagccgt tgccggtgcg ccgggtaaat caggttacaa cgttccggca
     2701 atcctcgaag ccttaggcgg tgccgacaat attgttagcc tcgataactg cattacccgt
     2761 ctgcgtttgt ctgtgaaaga tatgtcgctt gttaatgtgc aggcactgaa ggacaatcgg
     2821 gcaattggcg tagtacaact taatcaacat aacctgcagg ttgttatcgg gccacaagtt
     2881 cagtcagtaa aagatgaaat ggccggtctg atgcatactg tccaggcata aggataagat
     2941 atgttcgatt tttcaaaggt cgtggatcgt catggcacat ggtgtacaca gtgggattat
     3001 gtcgctgacc gtttcggcac tgctgacctg ttaccgttca cgatttcaga catggatttt
     3061 gccactgccc cctgcattat cgaggcgctg aatcagcgcc tgatgcacgg cgtatttggc
     3121 tacagccgct ggaaaaacga tgagtttctc gcggctattg cccactggtt ttccacccag
     3181 cattacaccg caattgatac gcagacggtg gtgtatggcc cttctgtcat ctatatggtt
     3241 tcagaactga ttcgtcagtg gtctgaaaca ggtgaaggcg tggtgatcca cacgcccgcc
     3301 tatgacgcat tttacaaggc cattgaaggt aaccagcgca cagtaatgcc cgttgcttta
     3361 gagaagcagg ctgacggctg gttttgcgat atgggcaagc tggaagccgt tttggcgaaa
     3421 ccagaatgta aaattatgct cctgtgtagc ccacagaatc ctaccgggaa agtgtggacg
     3481 tgcgatgagc tggagatcat ggctgacctg tgcgagcgtc atgatgtgcg ggttatttcc
     3541 gatgaaatcc atatggatat ggtctggggc gagcagccgc atattccctg gagtaatgtg
     3601 gcgcggggag actgggcgtt gctgacgtcg ggatcgaaaa gtttcaatat tcccgcgctg
     3661 accggtgctt acgggattat agaaaatagc agcagccgtg atgcctattt atcggcactg
     3721 aaaggacgtg atgggctttc ttccccttcg gtactggcgt taactgccca tatcgccgcc
     3781 tatcagcaag gtgcaccgtg gctggatgcc ttacgcgtct atctgaaaga taacctgacg
     3841 tatatctcag ataaaatgaa cgccgcgttt cctgaactca actggcagat cccgcaatcc
     3901 acttatctgg cctggcttga tctacgtccg ttgaatattg acgacaacgc gttgcaaaaa
     3961 gcgcttatcg agcaagaaaa agtcgcgatc atgccggggt atacctatgg tgaagaaggt
     4021 cgtggttttg tccgtctcaa tgccggctgc ccacgttcga aactggaaaa aggtgtggct
     4081 ggattaataa acgccatccg cgctgttcgc taaccccaat tgcgcaacgt aaaaaatcgt
     4141 tgcgcaatcg tggattttta ccctgctttg tttttataat ggtgcgcact tttatatcca
     4201 gaaaaagagt gcgaccatga ttgataccac cctgccatta actgatatcc atcgccacct
     4261 tgatggcaac attcgtcccc agaccattct tgaacttggc cgccagtata atatctcgct
     4321 tcctgcacaa tccctggaaa cactgattcc ccacgttcag gtcattgcca acgaacccga
     4381 tctggtgagc tttctgacca aacttgactg gggcgttaaa gttctcgcct ctcttgatgc
     4441 ctgccgccgc gtggcatttg aaaacattga agatgcagcc cgtaacggcc tgcactatgt
     4501 cgagctgcgt ttttcaccag gctacatggc aatggcacat cagctgcctg tagcgggtgt
     4561 tgtcgaagcg gtgatcgatg gcgtacgtga aggttgccgc acctttggtg tgcaggcgaa
     4621 gcttatcggc attatgagcc gaaccttcgg cgaagccgcc tgtcagcaag agctggaggc
     4681 ctttttagcc caccgtgacc agattaccgc acttgattta gctggtgatg aacttggatt
     4741 cccgggaagt ctgttccttt ctcacttcaa ccgcgcgcgt gatgcgggct ggcatattac
     4801 cgtccatgca ggcgaagctg ccgggccgga aagcatctgg caggcgattc gtgaactggg
     4861 tgcggagcgt attggacatg gcgtaaaagc cattgaagat cgggcgctga tggattttct
     4921 cgccgagcaa caaattggta ttgaatcctg tctgacctcc aatattcaga ccagcaccgt
     4981 agcagagctg gctgcacatc cgctgaaaac gttccttgag catggcattc gtgccagcat
     5041 taacactgac gatcccggcg tacagggagt ggatatcatt cacgaatata ccgttgccgc
     5101 gccagctgct gggttatccc gtgagcaaat ccgccaggca cagattaatg gtctggaaat
     5161 ggctttcctc agcgcagagg aaaaacgcgc actgcgagaa aaagtcgccg cgaagtaaca
     5221 aaaatggatg gtgcaatttg caccatccat ttttcacgca aggcacaacg tcgcgcgatg
     5281 tttggcggat tcgatgccca gctcaatcaa ctccattacc tggattgcct ggcttgccgg
     5341 aaccggattt tcaccatcgc catttaacgc atcacgaatc gccgcatagt aagccggata
     5401 attcccaggc accgtcaaca gcgtttcttc gacacgttcc tcaccttcca cgcgggtaag
     5461 tacgccatca cgcatatcgt agccccagtc ttcctgcggc agacgctcgc catttttcag
     5521 acgttcttcc tgtggatcga ggccatattt cacacaactg cctcgggatc catgcacgat
     5581 ataccgtgct gactcagcgg ctgccagcat ggtaccgtgt aaaatgactc gccgctgtgg
     5641 ataggacaag atggcgtgga aataatcggt cgactgcgct ccgggccgta actgtgccaa
     5701 atctaccgtc atgctgaccg gtaaaccaaa tagcgtaatg gcctgatcaa gaagatgcgg
     5761 tgctaaatcg taccagatac cgctgcctgg gccgccctgt tcacgccaac gatcgcgcac
     5821 ctgcggacgg aagcggtcaa aatgagactc aaagtaagca acttcaccca gcacgccttc
     5881 cgcgagcaaa ccttttagcg tcaagaaatc gctatcccaa cgacggttat ggaatacaga
     5941 cagcacacgc cccaggcttt ttgccagcgc atccagctct cgcgcttgtg acagtgtcac
     6001 ggtaaagggt ttatcaacga ccacatgttt acccgcctca agcgccgctt tggctaacgg
     6061 gaaatgggta tcgttgggtg taggaatgac aatcaggtct atgttgggat cgttaaacag
     6121 atgcttcggc tcagagacaa ccgtaaccgt tggccagtcg gcttttactt ttgtttcatc
     6181 actgctggag attaccgcca gttccagccc gggcgtgccc gcaatcaggg gcgcatggaa
     6241 ggttttgctc gcataaccat acccaatcaa cccaacacgg atgttgtcgc tcatgtcatt
     6301 gcctctcatt gaagtatgat ggctatttga cactatcctt tacccacgct caacagttta
     6361 ataacctgcc agcaataagg gatgttgttt aacttaagtc aaaaaaaata gcgaattttc
     6421 caacgacaaa agctaaatat cgcaaaaacc tcagtaaaaa tcttgctgga gctattattg
     6481 ctaagtaaca tttaccccct gaagttaatg gatcaatcaa gagagatgtg ggctgtaatg
     6541 aatcgtctta ttgaattaac aggttggatc gttcttgtcg tttcagtcat tcttcttggc
     6601 gtggcgagtc acattgacaa ctatcagcca cctgaacaga gtgcttcggt acaacacaag
     6661 taagctctgc acttgtggag cgacatgctg cccgtccggg tgcatgtttt cacttgtcgg
     6721 atattaaacc aggaatttat tatcttgttc gatgttgttg gtgattgtca gggatagtaa
     6781 agcgcgttac tcttttcaga gcagtttttc atccctgaat atttgccatc agcgcattct
     6841 tattattgta ataacacaag cgttcacttt tggtgaaatt ttatcatcac tctacccctt
     6901 tggtattaat tattttatgg accttttatg actgttcagg actacttatt aaaatttcgc
     6961 aaaatcagtt cactcgaaag tctggaaaaa ctctacgacc atcttaatta caccctgacg
     7021 gacgatcagg aactgatcaa tatgtatcgt gctgccgatc accgtcgcgc agagctggtt
     7081 tctggcgggc gtttgtttga cctcggccag gtaccgaagt ccgtctggca ctatgtccaa
     7141 taaagaaagt agcgatcggt tataaaagcc ttataataac gcctctaaaa ttagatttgc
     7201 ctgtttgcgt cgctattctg gagaacctat gaccaccaca acgccacaac gtattggagg
     7261 ctggttgctt ggcccgttgg cctggctttt agtcgctctg ttgagtacga cgctggcgtt
     7321 gttgctgtat accgctgcgt tatcttctcc gcaaacattt caaacgctcg gcggacaagc
     7381 actgacgaca caaatcttat ggggcgtttc ttttattacc gctatcgcca tgtggtatta
     7441 cacactgtgg ttgaccatcg ctttctttaa acgtcggcgt tgtgttccta aacactatat
     7501 tatctggctg cttatttccg tattgctggc agtaaaagca ttcgcttttt cacccgtgga
     7561 agatggcata gccgttcgcc agttgctgtt tactttgttg gcaaccgcat tgatcgtccc
     7621 ctatttcaaa cgctcgtcgc gggtaaaagc gacgtttgtg aatccgtaat aaccttacag
     7681 ttaacctgtt gtcgcctgct ctggattaac ggataatagg cggctttttt atttcaggcc
     7741 gaaaaatgac tgactacctg ttactgtttg tcggaactgt actggtcaat aactttgtac
     7801 tggtcaagtt tctcggtctc tgtccgttta tgggggtttc caaaaaactg gaaaccgcga
     7861 tgggcatggg gctggcaaca acgtttgtga tgacgctggc gtctatttgc gcctggctta
     7921 tcgatacgtg gattttgatc ccacttaatc tgatttacct gcgcaccctg gcatttattc
     7981 tggtgattgc tgtggtcgtg cagttcaccg agatggtggt gcgcaaaacc agcccggtgc
     8041 tttaccgtct gctggggatt tttttgccgc ttatcaccac caactgtgca gtgctcggcg
     8101 tggcgttgct gaatatcaat ctcgggcaca atttcttgca gtcggcgctg tacggttttt
     8161 ccgccgctgt cggtttctcg ctggtgatgg tgctcttcgc cgccatccgc gaacgccttg
     8221 ctgtggctga tgtcccggca ccttttcgcg gtaatgccat tgcgttaatt accgcaggtc
     8281 ttatgtctct ggcctttatg ggctttaatg gtttggtgaa gttgtaatga atgctatctg
     8341 gattgccgtt gccgccgtga gcctgctggg cctggcgttt ggcgccattc tgggttatgc
     8401 ctcccgccgt tttgcggtgg aagacgatcc ggtcgttgag aaaattgacg aaatcttacc
     8461 gcagagccag tgtggtcagt gcggttatcc cggctgtcgc ccctacgcgg aagccatcag
     8521 ctgtaacggt gaaaaaatca accgttgcgc cccaggtggc gaagctgtga tgctaaaaat
     8581 tgccgagttg cttaatgtcg agccgcagcc gctggatggc gaagcgcaag agctaacgcc
     8641 tgcgcggatg gtggcggtta ttgatgaaaa taactgtatt ggctgcacta aatgtattca
     8701 ggcttgtccg gtagacgcca tcgttggcgc tacccgtgcc atgcatacgg taatgagtga
     8761 tctctgtacg ggctgcaatt tatgtgttga tccgtgcccg acgcactgca tctcgttgca
     8821 accggtcgca gaaacacctg actcctggaa atgggatctg aacaccattc ccgtgcgtat
     8881 cattcccgtg gaacaccatg cttaagttat tctctgcatt cagaaaaaat aaaatctggg
     8941 atttcaacgg cggcatccat ccaccggaga tgaaaaccca gtccaacggt acacccctgc
     9001 gccaggtacc actggcgcag cgttttgtta ttccactgaa acagcatatt ggcgctgaag
     9061 gtgagttgtg cgttagcgtc ggcgataaag tattgcgcgg ccagccgctt acccgtggtc
     9121 gcggcaaaat gctgcctgtt cacgcgccca cctcgggtac cgttacggct attgcgcccc
     9181 actctacggc tcatccttca gctttagctg aattaagcgt gattattgat gccgatggtg
     9241 aagactgctg gatcccgcgc gacggctggg ccgattatcg cactcgcagt cgcgaagagt
     9301 taatcgagcg catacatcag tttggtgttg ccgggctggg cggtgcagga ttcccgacag
     9361 gcgttaaatt gcagggtggc ggagataaga ttgaaacgtt gattatcaac gcggctgagt
     9421 gcgagccgta cattaccgcc gatgaccgtt tgatgcagga ttgcgcggct caggtcgtag
     9481 agggtattcg cattcttgcg catattctgc agccacgcga aattcttatc ggcattgaag
     9541 ataacaaacc gcaggcgatt tccatgctgc gcgcggtgct ggcggactct aacgatattt
     9601 ctctgcgggt gattccaacc aaatatcctt ctggcggtgc taaacaatta acctacattc
     9661 tgaccgggaa gcaggttcca catggcgggc gttcatccga tatcggcgta ttaatgcaaa
     9721 acgtcggcac tgcttatgca gtgaaacgtg ccgttattga tggcgagccg attaccgagc
     9781 gtgttgtaac cctgactggc gaagcaatcg ctcgcccggg caacgtctgg gcacggctgg
     9841 ggacgccagt gcgtcattta ttgaatgatg ccggattctg cccctctgcc gatcaaatgg
     9901 tgattatggg tggcccgcta atgggcttta ccttgccatg gctggatgtc ccggtcgtaa
     9961 agattaccaa ctgtctgttg gctccctctg ccaatgaact tggcgaacca caggaagaac
    10021 aaagctgcat ccggtgtagc gcctgtgctg acgcctgccc tgcggatctt ttgccgcaac
    10081 agttgtactg gttcagcaaa ggtcagcaac acgataaagc taccacgcat aacattgctg
    10141 attgcattga atgtggggct tgcgcgtggg tttgcccgag caatattccc ctggtgcaat
    10201 atttccgtca ggaaaaagct gaaattgcgg ctattcgtca ggaagaaaag cgcgccgcag
    10261 aagccaaagc gcgtttcgaa gcgcgccagg ctcgtctgga gcgcgaaaaa gcggctcgcc
    10321 ttgaacgaca taagagcgca gccgttcaac ctgcagccaa agataaagat gcgattgctg
    10381 ccgctctggc gcgggtgaaa gagaaacagg cccaggctac acagcctatt gtgattaaag
    10441 cgggcgaacg cccggataac agtgcaatta ttgcagcacg ggaagcccgt aaagcgcaag
    10501 ccagagcgaa acaggcagaa ctgcagcaaa ctaacgacgc agcaaccgtt gctgatccac
    10561 gtaaaactgc cgttgaagca gctatcgccc g
//
LOCUS       DAJTZL010000140        10420 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.140, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000140 DAJTZL010000000
VERSION     DAJTZL010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10420
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.140"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            142..2010
                     /gene="kup"
                     /locus_tag="OO544_003147"
     CDS             142..2010
                     /gene="kup"
                     /locus_tag="OO544_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HCR6768676.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAMHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            2177..2596
                     /gene="rbsD"
                     /locus_tag="OO544_003148"
     CDS             2177..2596
                     /gene="rbsD"
                     /locus_tag="OO544_003148"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HCR6768677.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            2604..4109
                     /gene="rbsA"
                     /locus_tag="OO544_003149"
     CDS             2604..4109
                     /gene="rbsA"
                     /locus_tag="OO544_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HCR6768678.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVIALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLCFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKSQGRGIVYISHRMKEIFEICDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGAQPRTSGYVTLDGHEVVTRSPQDGLANGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            4114..5079
                     /gene="rbsC"
                     /locus_tag="OO544_003150"
     CDS             4114..5079
                     /gene="rbsC"
                     /locus_tag="OO544_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HCR6768679.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            5104..5990
                     /gene="rbsB"
                     /locus_tag="OO544_003151"
                     /pseudo
     CDS             5104..5990
                     /gene="rbsB"
                     /locus_tag="OO544_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
     gene            6116..7045
                     /gene="rbsK"
                     /locus_tag="OO544_003152"
     CDS             6116..7045
                     /gene="rbsK"
                     /locus_tag="OO544_003152"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HCR6768680.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            7049..8041
                     /gene="rbsR"
                     /locus_tag="OO544_003153"
     CDS             7049..8041
                     /gene="rbsR"
                     /locus_tag="OO544_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HCR6768681.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(8007..9434)
                     /gene="mdtD"
                     /locus_tag="OO544_003154"
     CDS             complement(8007..9434)
                     /gene="mdtD"
                     /locus_tag="OO544_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HCR6768682.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARHTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVSFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNRVPSESE"
     gene            complement(9457..10149)
                     /locus_tag="OO544_003155"
     CDS             complement(9457..10149)
                     /locus_tag="OO544_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HCR6768683.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLNLHQAIVDAIVQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         2548 a         2514 c         2753 g         2605 t
ORIGIN      
        1 aagggggggt atttttcttt gagccaggtt aatgtggccg catttaggag tacgattttg
       61 ccgttaatcg tgcatactgt gcgctttttt gtgggccaag ggactaagca cacatttcat
      121 atttcaacga aagactagtc tatgagcact gataataagc aatcattgcc cgcgattacc
      181 ctcgcggcga ttggagttgt ctacggcgat attggtacca gcccgttata tacacttcgt
      241 gaatgtttgt ccggccagtt tggttttggc gttgaacgcg atgccgtgtt tggcttttta
      301 tcgctgatct tctggctgct aatctttgtg gtttccatta aatatctcac cttcgtgatg
      361 cgggcagata acgccggtga gggggggatc ctgacgttga tgtcgcttgc cgggcgtaat
      421 acgtcggcgc gaaccacatc aatgctggtg attatggggc taatcggcgg cagctttttc
      481 tatggtgaag tcgtcataac acccgctatt tcggtgatgt cagccattga aggtctggaa
      541 atcgtcgccc cgcagctgga tacctggata gttcccctct caattatcgt tctcacttta
      601 ttatttatga ttcaaaagca tggcacggct atggtcggta agctgtttgc gccgatcatg
      661 ctgacctggt ttttgattct ggcaggactg gggttacgta gcattattgc taacccggaa
      721 gtgctgcatg cgctgaatcc gatgtgggcg atgcatttct tccttgaata caaaacggtt
      781 tcttttattg cattaggggc agtggtgttg tcgattacgg gggtcgaggc gctgtatgct
      841 gatatggggc actttggtaa gttccctatt cgcctggcgt ggttcaccgt cgtattacct
      901 tccttgaccc ttaattactt cggtcaggga gcgctgttgt taaagaaccc ggaagcgatt
      961 aagaacccgt tcttcctgtt ggcaccggac tgggcgctga tcccgctgct gatcatcgcc
     1021 gcactggcga cggtaattgc ctcgcaggcg gttatctctg gcgtcttctc attgacgcgt
     1081 caggcggtac gtctgggata tttgtcgccg atgcgcatta ttcacacctc cgaaatggag
     1141 tcagggcaaa tctatattcc gtttgtgaac tggatgctct atgtcgcggt cgtgattgtg
     1201 attgtcagct ttgagcactc cagcaacctg gcggcggcgt acgggattgc ggtgaccgga
     1261 accatggtgc tgacgtctat tctctcgact accgtggcac gtcagaactg gcactggaat
     1321 aagtattttg ttgcgctgat cctgattgct ttcctttgtg tcgatattcc attgttcacc
     1381 gctaacctcg ataaactgct ctccggcggc tggttgccat tgagcctcgg tactgtgatg
     1441 tttatcgtga tgaccacctg gaagagcgag cgtttccgct tgctgcggcg gatgcatgaa
     1501 catggtaact ctctggaagc gatgattgct tcgctggaga aatcaccgcc tgttcgcgtg
     1561 cccgggaccg cggtgtatat gtcgcgtgca atcaacgtca ttccctttgc gctgatgcat
     1621 aaccttaaac ataacaaggt attgcatgag agggtgattc tgttaactct gcgcaccgaa
     1681 gacgcgccat atgtccataa cgtccgtcgg gtacagattg aacaactgtc gcccactttc
     1741 tggcgcgtgg tggcaagtta tggttggcga gaaacgccaa acgtggaaga agttttccac
     1801 cgctgcggtc tggaaggatt aagttgccgg atgatggaaa cctccttctt tatgtcgcat
     1861 gagtcgttga tcctcggcaa acgcccgtgg tatttgcgtc tgcgcggcaa gctgtacttg
     1921 ctgctgcaac gtaatgcgct gcgtgcacca gatcaatttg aaatcccgcc aaacagggtt
     1981 atcgaactgg gtactcaggt cgaaatctaa cgctagacgc ctcctttctc cataaggggg
     2041 cgtttttgtt ttcatggtta atcaccatgt aaaacgtttc gaggttgatc acatttccgt
     2101 aacgtcacga tggttttccc aactcagtca ggattaaact gtgggtcagc gaaacgtttc
     2161 gctgatggag aaaaaaatga aaaaaggcac cgttcttaat tctgatattt catcggtgat
     2221 ctcccgtctg ggacataccg atacgctggt ggtgtgtgat gctggtttac ccatccccaa
     2281 aagtacaacg cgtatcgata tggcattaac ccagggtgta ccttctttta tgcaggtgct
     2341 gggcgtcgtc acaaatgaaa tgcaggtcga ggctgccatt atcgcggaag agatcaaaca
     2401 acataatccg caactccacg aaacgttgct cactcacctt gagcagctgc aaaaacacca
     2461 gggaaatacc attgaaattc gttacaccac gcatgaacaa ttcaaacaac aaaccgcaga
     2521 aagtcaggcg gtaattcgca gcggagaatg ttctccgtat gcgaatatca ttctctgtgc
     2581 tggcgtgacg ttctgaggcc gtcatggaag cattacttca gcttaaaggc atcgataaag
     2641 ccttcccggg cgtaaaagcc ctctcgggcg ctgcgttaaa tgtctatccg ggccgcgtga
     2701 tagcgctggt gggcgaaaac ggcgcgggta aatccaccat gatgaaagtg cttactggta
     2761 tctatactcg cgatgccggc acgcttttat ggctggggaa agaaacgaca tttaccgggc
     2821 cgaaatcttc ccaggaagcc gggattggga ttatccatca ggaactgaac ctgatcccgc
     2881 agttgaccat tgccgaaaac attttcctcg gtcgtgagtt tgttaatcgc tttggcaaaa
     2941 ttgactggaa aaccatgtat gccgaagcgg ataaattgct ggctaaactt aacctgtgct
     3001 ttaaaagcga caagctggtg ggcgatcttt ccatcggtga ccagcaaatg gttgaaatcg
     3061 ccaaagtgct gagctttgag tcgaaagtca tcattatgga tgaaccgacc gatgcgctga
     3121 ccgataccga aaccgaatcc ctgttccgcg tcatccgcga gctgaaatcg caaggccgcg
     3181 gtattgtcta tatctcccac cgcatgaaag aaatcttcga gatttgcgat gacgttaccg
     3241 tttttcgtga cgggcaattt attgctgagc gcgaagtggc atcactgacc gaagattcgc
     3301 tgattgagat gatggtgggc cgcaagctgg aagatcaata tccacacctg gacaaagcgc
     3361 cgggagatat ccgcctgaaa gtcgataatc tctgcggacc tggcgttaac gatgtctctt
     3421 ttactttacg caaaggcgaa attcttggcg tctctggttt gatgggcgca ggtcgtaccg
     3481 aactgatgaa agtgctctac ggcgcacagc cgcgcaccag cggttacgtc accctggatg
     3541 ggcatgaagt cgttacccgt tcaccgcagg atggcctggc aaacggcatt gtgtatatct
     3601 ccgaagaccg taaacgtgac ggtttagtgt tgggcatgtc agtaaaagag aacatgtcgc
     3661 tgacagcgct gcgctacttc agccgcgctg gcggcagttt gaagcatgcc gatgaacagc
     3721 aggcagtgag tgatttcatt cgtctgttta atgtgaaaac accgtcgatg gaacaggcaa
     3781 ttggtctgct ttccggtggc aatcagcaaa aagtggcgat tgcccgcggt ctgatgacac
     3841 gccccaaagt gttgatcctt gatgaaccta cccgtggcgt agatgtcggc gcgaaaaaag
     3901 agatctatca actgattaac cagttcaaag ccgatggctt gagcatcatt ctggtgtcat
     3961 cggagatgcc agaagtatta ggcatgagcg atcgcatcat cgtcatgcat gaagggcatc
     4021 tcagcgggga atttactcgt gagcaggcca cccaggaagt gttaatggct gccgctgtgg
     4081 gcaagcttaa tcgcgtgaat caggagtaaa aaaatgacaa cccagactgt ctctggtcgc
     4141 cgttatttca cgaaagcgtg gctgatggag cagaaatcgc ttatcgctct gctggtgctg
     4201 atcgcgattg tctcgacgtt aagcccgaac tttttcacca tcaataactt attcaatatt
     4261 ctccagcaaa cctcggtgaa cgccattatg gcggtcggga tgacgctggt gatcctgacg
     4321 tcgggcatcg acttgtctgt aggttctctg ttggcgctaa ccggcgcagt tgctgcatct
     4381 atcgtcggca ttgaagtcaa tgcgctggtg gctgtcgctg ctgctctcgc gttaggtgcc
     4441 gcaattggtg cggtaaccgg ggtgattgta gcgaaaggtc gcgttcaggc gtttatcgct
     4501 acgctggtta tgatgctttt actgcgcggc gtgaccatgg tttataccaa cggtagccca
     4561 gtgaataccg gctttactga gaacgccgat ctgtttggct ggtttggtat tggtcgtccg
     4621 ctgggcgtac cgacgccagt ctggatcatg gggattgtct tcctcgcggc ctggtacatg
     4681 ctgcatcaca cgcgtctggg gcgttacatc tacgcgctgg gcggcaacga agcggcaacg
     4741 cgtctttctg gtatcaacgt caataaaatc aaaatcatcg tctattctct ttgtggtctg
     4801 ctggcatcgc tggccgggat cattgaagtg gcgcgtctct cctccgcaca acccacggcg
     4861 gggactggct atgagctgga tgctattgct gcggtggttc tgggcggtac gagtctggcg
     4921 ggcggaaaag gtcgcattgt tgggacgttg atcggcgcat taattcttgg cttccttaat
     4981 aatggattga atttgttagg tgtttcctcc tattaccaga tgatcgtcaa agcggtggtg
     5041 attttgctgg cggtgctggt agacaacaaa aagcagtaat aacgactaca ggacatcttg
     5101 aatatgaaca tgaaaaaact ggctaccctg gtttccgctg ttgcgctaag cgccaccgtc
     5161 agtgcgaatg cgatggcaaa agacaccatc gcgctggtgg tctccacgct taacaacccg
     5221 ttctttgtat cgctgaaaga tggcgcgcag aaagaggcgg ataagcttgg ctataacctg
     5281 gtggtgctgg actcccagaa caacccggcg aaagagctgg cgaacgtgca ggacttaacc
     5341 gttcgcggca ccaaaattct gctgattaac ccgaccgact ccgacgcagt aggtaatgct
     5401 gtgaagatgg ctaaccaggc gaacatcccg gttatcactc ttgaccgcca ggcaacgaaa
     5461 agtgaagtgg tgagccacat tgcttctgat aacgtactgg gtggcaaaat cgccggtgat
     5521 tacatcgcga agaaagcggg tgaaggtgcc aaagttatcg agctgcaagg cattgctggt
     5581 acatccgcag cccgtgaacg tggcgaaggc ttccagcagg ccgttgctgc tcacaagttt
     5641 aatgttcttg ccagccagca gattttgatc gcactaaagg tttgaacgta atgcagaacc
     5701 tgttgaccgc tcatccggat gttcaggctg tattcgcgca gaatgatgaa atggcgctgg
     5761 gcgcgctgcg cgcactgcaa actgccggta aatcggatgt gatggtcgtc ggatttgacg
     5821 gtacaccgga tggcgaaaaa gcggtgaatg atggcaaact agcagcgact atcgctcagc
     5881 tacccgatca gattggcgcg aaaggcgtcg aaaccgcaga taaagtgctg aaaggcgaga
     5941 aagttcaggc taagtatccg gttgatctga aactggttgt taagcagtag ttttaatcag
     6001 gttgtatgac ctgatggtga cataaatacg tcatcgacag atgaacgtgt aatataaaga
     6061 aaagcagggc acgcgccacc ctaacacggt ggcgcatttt atggacatcc cgaatatgca
     6121 aaacgcaggc agcctcgttg ttcttggcag cattaatgct gaccacattc ttaatcttca
     6181 atcttttcct actccaggcg aaaccgtaac cggtaaccac tatcaggttg catttggcgg
     6241 caaaggcgcg aatcaggctg tggctgctgg gcgtagcggt gcgaatatcg cgtttattgc
     6301 ctgtacgggt gatgacagca ttggtgagag cgttcgccag cagctcgcca ctgataacat
     6361 tgatattact ccggtcagcg tgatcaaagg cgaatcaaca ggtgtggcgc tgatttttgt
     6421 taatggtgaa ggtgagaatg tcatcggtat tcatgccggc gctaatgctg ccctttcccc
     6481 ggcgctggtg gaagcgcaac gtgagcgtat tgccaacgcg tcagcattat taatgcagct
     6541 ggaatcacca ctcgaaagtg tgatggcagc ggcgaaaatc gcccatcaaa ataagactat
     6601 cgttgcgctt aacccggctc cggctcgcga acttcctgac gaactgctgg cgctggtgga
     6661 cattattacg ccaaacgaaa cggaagcaga aaagctcacc ggtattcgtg ttgaaaatga
     6721 tgaagatgca gcgaaggcgg cgcaggtact gcatgaaaaa ggtatccgta ctgtactgat
     6781 tactttagga agtcgtggtg tatgggctag cgtgaatggt gaaggtcagc gcgttcctgg
     6841 attccgggtg caggctgtcg ataccattgc tgccggagat acctttaacg gcgcgttaat
     6901 cacggcgttg ctggaagaaa aaccattgcc agaggcgatt cggtttgccc atgctgccgc
     6961 tgcgattgcc gtaacacgta aaggcgcaca accttccgta ccgtggcgtg aagagatcga
     7021 cgcattttta gacaggcaga ggtgacgctt ggctacaatg aaagatgttg cccgcctggc
     7081 gggcgtttct acctcaacag tttctcacgt tatcaataaa gatcgcttcg tcagtgaagc
     7141 gattaccgcc aaagttgaag cggcgattaa agaactcaat tacgcgccat cagccctggc
     7201 gcgtagcctc aaactcaatc aaacacatac cattggcatg ttgatcactg ccagtaccaa
     7261 tcctttctat tcagaactgg tgcgtggcgt tgaacgcagc tgcttcgaac gcggttatag
     7321 tctcgtcctt tgcaataccg aaggcgatga acagcggatg aatcgcaatc tggaaacgct
     7381 gatgcaaaaa cgcgttgatg gcttgctgtt actgtgcacc gaaacgcatc aaccttcgcg
     7441 tgaaatcatg caacgttatc cgacagtgcc tactgtgatg atggactggg ctccgttcga
     7501 tggcgacagc gatcttattc aggataactc gttgttgggc ggagacttag caacgcaata
     7561 tctgatcgat aaaggtcata cccgtatcgc ctgtattacc ggtccgctgg ataaaactcc
     7621 ggcgcgcctg cggttggaag gttatcgggc ggcgatgaaa cgtgcgggtc tcaacattcc
     7681 tgatggctat gaagtcactg gtgattttga atttaacggc gggtttgacg ccatgcgcca
     7741 actgttatca catccgctgc gtcctcaggc cgtctttacc ggaaatgacg ctatggctgt
     7801 tggcgtttac caggcgttat atcaggcaga gttacaggtt ccgcaggata tcgcggtgat
     7861 tggctatgac gatatcgaac tggcaagctt tatgacgcca ccattaacca ctatccacca
     7921 accgaaagat gaactggggg agctggcgat tgatgtactc atccatcgga taacccagcc
     7981 gacccttcag caacaacgat tacaacttac tccgattctg atggaacgcg gttcggctta
     8041 gatttacgct gtcttttgat caaattatta ccatcggttg ttttcagaag catgaacatt
     8101 gctgctgaag caacagtaat aatgcccatc gtgataaacg tatagtggaa ttgttcgaca
     8161 gtcgttgtgc cttccatccc ttcataaacg cgaaggacgg ccgcacttac agcaacgcct
     8221 aaactaatcg acagttgctg cgtgaccgcc agaacactgt taccgctgct ggcgttgtca
     8281 tcggtcagat cggcaagtgt gatggtattc atcgcggtaa attgcgtcga catagccatc
     8341 cctaatataa acaacggcaa gatcagcatc catatcgcca ttgccggtga ttgcaaagag
     8401 aactgagcga tcattagccc aataatcacc gtgatcccca ctaacgtatg gcgatagccc
     8461 agacgacgta agacttgggt aaccatcgat tttgcaataa tggaacctaa cgctgtcggt
     8521 gccatcatac agccggcaat aaacgcctga taaccaaatc ctacctgtaa catcaatggc
     8581 ataaggaacg atacacaacc ggtccccaga cgggttgcaa tattgcctac gataccgatt
     8641 gagaaagtgc gggttttaaa taaatctaat gaaattaatg ggtttggcgt gtgtcgcgca
     8701 tggagaatat agagaagcag taacccgatg ctggtgacaa ttaccgtcaa ggcaatccag
     8761 ctggcgacaa tcttttcccc gaatagctct attccgcttg agaagagaac aaggctgagg
     8821 ccaaacagca aaaagccagt gatatcgaat ctgcgtcgtg cggtggtgaa attgggcata
     8881 tgtttgcgcg cgtaaagaag gcccgcaata cctatgggga tattgattaa aaatatccag
     8941 tgccaggttg cccaggtgac cagcacgccg ccaagaacgg ggcctaaaat tggccccacc
     9001 agacccggca tggcgacaaa attcaatact ggaagaagtt cattacgagg ataagcgcgc
     9061 agtaaggcca gccgagcaac aggcatcatc attgcgccgc ctatcccctg aataacccgg
     9121 aagacaacca gctgtggtag cgaattagaa agtgcgcagg ccagagaacc caatgtgaac
     9181 agactcacgg caagggtaaa aatgcgacgc gtaccgaagc gatcggctag ccatccgctt
     9241 accggaataa gcatcgccac cgtcagcgta taactgatga tggctgattg catcgcgaga
     9301 ggagaacgat taaggctatg agcgattgcg ggtaaggcgg tattcagaat agtggcatca
     9361 agtgcctgca tgaagaaggc catcgccgcg atccacggca aacccgccat actgcgcttc
     9421 tttttatcgc tcattcaatg tcttgttatc gggttgtcac ttatcaggtg agcgtagcag
     9481 cgcctgacaa gctttaaatg ccgcgtcgcc atcgctttgg acaatcgcat cgacaatcgc
     9541 ctggtgcaga tttagcttta tcactgtgtc gctggtaatt gacgtgaagt aagtgtgata
     9601 aaccgaatgg aatagcgagg cgaatgaggt caaaaacgga ttggcgctca tttcatagat
     9661 atgctcatgc caggccatat cgacttcgat ccagcgttca cggcgaaagt tctcttttaa
     9721 tgccgccatt tcggccatta acgtattgag atgcgccttc tgttccgcgg tgccaaccgt
     9781 tgctgccagt aggcaggctt gcggctccag acagatacgc ataaccagaa agtgatcgat
     9841 gacctgatga aagttctctt ctgtcatcca ccaggtaagc aattcctgat caagaaaatt
     9901 ccagttcgat tgtggcatga cccgagtacc aattcgcggt cgcggtaaaa ccatcccttt
     9961 tgccgttaac gttttgaccg cttcgcgtac cgctgtacga ctcactccaa attgctcgcc
    10021 cagctcaatc tcaccaggca aaatggtgcc gggttcatat tcacctttta agatccgttg
    10081 cgccagcttc tcagccagaa catacgaaag gtttttctgt gcagctaact gttgtgcgct
    10141 taaaggcatt acttatcttc ctttttcttt ttattcctcc ttagtatgcc accaggaagt
    10201 gtgattacgg ttgcaaaaac ggcaaattgc ttgttttatg gtacattaac ggggcttttg
    10261 ctgaaaaaat gcgcggtcag aaaattattt taaatttcct cttgtcaggc cggaataact
    10321 ccctataatg cgccaccact gacacggaac aacggcaaac acgccgccgg gtcagcgggg
    10381 ttctcctgag aatctcaaca gagaaagcaa aaataaatgc
//
LOCUS       DAJTZL010000141        10362 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.141, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000141 DAJTZL010000000
VERSION     DAJTZL010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10362
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.141"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..154
                     /locus_tag="OO544_003156"
     CDS             <1..154
                     /locus_tag="OO544_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR6768684.1"
                     /translation="TAENIQIELRDDQDAALKNGDSKTVIVDEITRNAQFPLKARAIT
                     VNGNAS"
     gene            258..1172
                     /locus_tag="OO544_003157"
     CDS             258..1172
                     /locus_tag="OO544_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6768685.1"
                     /translation="MGKTISIKVLFGIYLLLMAGKVFAFSCNVDGGSSIGAGTTSVYV
                     NLDPVIQPGQNLVVDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNN
                     VTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIATL
                     GSGNPRNFTWNIISNNSVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLGVYCSSEQKLS
                     FYLSGATTDSSRQVFANTAPDATKASGVGVTLMRNGKILATGENVSLGTVNKSKVPLG
                     LSATYGQTGNKVSAGTVQSVIGVTFIYE"
     gene            1506..3785
                     /gene="ydeP"
                     /locus_tag="OO544_003158"
     CDS             1506..3785
                     /gene="ydeP"
                     /locus_tag="OO544_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HCR6768686.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRHDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAADRTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFTPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPHS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            4033..4230
                     /gene="safA"
                     /locus_tag="OO544_003159"
     CDS             4033..4230
                     /gene="safA"
                     /locus_tag="OO544_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HCR6768687.1"
                     /translation="MYATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLG
                     ISEMGRIARDLTHFIINSLQD"
     gene            4305..5066
                     /gene="ydeO"
                     /locus_tag="OO544_003160"
     CDS             4305..5066
                     /gene="ydeO"
                     /locus_tag="OO544_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HCR6768688.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFTENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQSHMGMNTGNTMSALAI"
     gene            5467..7149
                     /locus_tag="OO544_003161"
     CDS             5467..7149
                     /locus_tag="OO544_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HCR6768689.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQDKKQGAPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            7201..8358
                     /locus_tag="OO544_003162"
     CDS             7201..8358
                     /locus_tag="OO544_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HCR6768690.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIEASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPLELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(8324..8446)
                     /locus_tag="OO544_003163"
     CDS             complement(8324..8446)
                     /locus_tag="OO544_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6768691.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
     gene            8655..10340
                     /locus_tag="OO544_003164"
     CDS             8655..10340
                     /locus_tag="OO544_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HCR6768692.1"
                     /translation="MITIPITFYMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMILG
                     VVKIQVWLNDWNNDFFNALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQ
                     SLSMLITFTVILWQSAGTLSFTVGGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRP
                     LNVEKQRSEATFRTNLVQHNKQTELIALSNAESLQRQELRDNFHTIKENWHRLMNRQR
                     WLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGLMKSRQAFMLVSNNLSWFIYKYDE
                     LAELAAVIDRLYEFHQLTEQRPTNKPKNCQHAVQVANASIRTPDNKIILENLNFHVSP
                     GKWLLLKGYSGAGKTTLLKTLSHCWPWFKGDISSPADSWYVSQTPLIKSGLLKEIICK
                     ALPLPVDDKSLSEVLHQVGLGKLAARIHDHDRWGDILSSGEKQRIALARLILRRPKWI
                     FLDETTSHLEEQEAIRLLRLVREKLPTSGVIMVTHQPGVWNLADDICDISTVL"
BASE COUNT         3028 a         2207 c         2214 g         2913 t
ORIGIN      
        1 tactgccgaa aatattcaga tagagctgag ggatgaccag gatgctgcgt taaaaaatgg
       61 cgatagcaaa acggttattg ttgatgagat cactcgtaat gcacagtttc cacttaaggc
      121 aagagctatc acggtgaatg gaaacgcaag ctagggaacg atcgaggcgc taatcaatgt
      181 gatctacacc tggcaataac gtcgattcga ttttcttagc cttcttttta aatcaatttt
      241 taaccttagg atatatcatg ggtaaaacaa tcagtataaa ggtcctgttc ggtatttatc
      301 ttctattaat ggcaggcaaa gtttttgcct tcagttgtaa tgtcgatggc gggtcaagta
      361 ttggagcagg tacaacctct gtttatgtta atctcgaccc tgtaatacag ccgggccaga
      421 atctggttgt agacttgtct cagcatataa gttgctggaa tgattacggc ggctggtacg
      481 acactgatca tataaacctg gtacaaggct cagctttcgc aggctcccta cagtcatata
      541 aaggatcgct gtactggaat aatgtgacct accccttccc gttgacgaca aataccaacg
      601 tattggatat tggtgataag acaccgatgc cattaccgtt aaaactctat attaccccag
      661 ttggggcggc cggtggagtt gtcataaaag cgggggaagt cattgcccgt attcatatgt
      721 ataaaattgc aacattagga agcggaaatc cgcgtaattt tacgtggaat attatttcaa
      781 ataacagtgt tgttatgcca acaggcggct gcaccgttga tagtcgtaat gtcactgtgg
      841 atctcccgga ctttcctgga agcgcagaaa taccccttgg cgtatattgc tcaagcgaac
      901 aaaagctctc attttatctt tcgggtgcca ccactgacag tagccgacaa gtgtttgcta
      961 acacagcacc tgatgcaaca aaagcatcgg gagtgggtgt cactctaatg cgtaatggaa
     1021 aaatactggc tacgggagaa aatgtctccc tggggacagt aaataaaagc aaagtacctc
     1081 tgggtttaag cgcaacctat ggacaaacag gaaacaaggt ttcagccgga accgtccagt
     1141 cagtcattgg tgtcactttt atctatgaat aattgtgatt ttgaccttag tctatgcgct
     1201 ccattcacga aaattgcgca gataattatt ttgtgaaggc tattatccta cacctgtaag
     1261 aaaatccgcg cacaattgaa gccagagtct tattttttat attgaaaaat atgttaattt
     1321 aaacactatg agagatgatg cttaacttat cgtatttaat ctattgttta acgataatag
     1381 attaaaaata gaaagagagt agtcccctac tcttatatat ccatgttggc gataatccct
     1441 ttgtatgtac tgtgcatcat cgctattaca aatcctaata attcatttcc acacaggata
     1501 agtagatgaa gaaaaaaatt gaatcctacc agggtgctgc aggtggttgg ggtgctgtta
     1561 aatccgtagc gaatgcagta cgtaagcaga tggatatacg ccatgatgtt attgccatgt
     1621 ttgacatgaa taagccagag ggctttgact gtccgggttg tgcatggcca gatcctaagc
     1681 acagtgcgtc attcgacatt tgtgaaaacg gcgcaaaagc aatcgcctgg gaagtcacgg
     1741 ataagcaggt aaatgcctct ttctttgctg agaatacggt tcaatcatta cttacctggg
     1801 gagatcacga gcttgaggct gcggggcgtc tcactcagcc tttgaaatat gatgccgtca
     1861 gcgactgtta caagccatta agctggcaac aagctttcga cgaaattggc gcacgccttc
     1921 aaagctatag tgatcccaat caggttgaat tctatacttc gggccgcact tccaatgaag
     1981 ctgcctttct ttatcagctt tttgcccgtg aatacgggag caataacttt cccgactgct
     2041 ccaacatgtg ccatgaaccg acaagcgtgg gtttggcagc gagtatcggt gtaggtaaag
     2101 ggaccgtgtt gctggaagac tttgagaagt gcgatttagt catttgcatt gggcataacc
     2161 ctggtacaaa ccaccctcgc atgctgactt cgttgcgcgc tttagtgaaa cggggagcga
     2221 aaatgatcgc catcaatcct ctacaggaac gtggcctgga gcgatttacc gcaccgcaaa
     2281 acccgtttga aatgctgacg aactctgaga ctcagttggc cagtgcctac tacaacgtgc
     2341 gcattggtgg cgatatggcg ttgctcaagg ggatgatgcg cctgttaatt gagcgcgatg
     2401 atgctgcaag tgccgcaggt cggccctcat tgcttgatga cgaatttatt caaacgcata
     2461 ccgtcggctt tgacgagcta cgccgtgacg ttctcaattc cgagtggaaa gatatcgaac
     2521 gtatttctgg actaagtcag acacaaatcg ccgaactggc tgacgcatat gccgctgccg
     2581 atcgcaccat tatctgttac ggaatgggga tcactcagca cgaacatggt acccagaatg
     2641 tacagcaact ggtcaatctg ctgttgatga aaggtaacat tggcaagcct ggtgcgggta
     2701 tctgcccact acgaggtcac tctaatgtac agggcgaccg aaccgtcggt atcaccgaga
     2761 aaccgtctgc agagtttctg gctcgcctgg gtgagcgcta tggcttcacc ccacctcatg
     2821 cacctggaca tgctgcaatt gccagcatgc aagcaatatg cacggggcag gctcgagcat
     2881 tgatctgcat ggggggcaac tttgcactgg caatgccaga tcgggaagcg agcgctgtac
     2941 cgttaacgca attagatttg gcggtacacg tagccactaa gcttaaccgc tctcatctgc
     3001 tgaccgcacg gcatagctat attctgccgg ttctggggcg tagcgagatt gacatgcaaa
     3061 aaagcggtgc gcaggcggta accgttgagg attcaatgtc gatgattcat gcctcgcgtg
     3121 gcgtgttaaa acccgccggt gtaatgctga aatcagagtg tgcagtggtc gcgggaatcg
     3181 cgcaggcagc actaccccac agcgtggtag cctgggagta tctggtggaa gattatgatc
     3241 gcattcgcaa tgacattgaa gctgtgttac cagagttcgc cgactataac cagcgcatcc
     3301 gacatcctgg aggttttcac ctgataaatg cagctgctga aaggcgctgg atgacgccgt
     3361 caggtaaggc taatttcatt accagcaaag ggctgttaga agatccctct tcagcgttta
     3421 acagtaagct ggtcatggcg acagtacgta gccacgatca gtacaacacg acgatttatg
     3481 gtatggatga tcgctatcga ggggtattcg gtcaacgaga tgtggtcttt atgagtgcta
     3541 aacaagctaa aatttgccgt gtaaaaaacg gcgaaagagt taatcttatt gcgcttacgc
     3601 cagacggtaa gcgcagctca cgccgcatgg atagattaaa agtggtcatt taccctatgg
     3661 ctgaccgctc actggtgacc tattttccag aatcgaatca catgctaact cttgataacc
     3721 acgatccatt aagtggcatt cctggttata aaagtattcc ggttgaatta gaaccatcaa
     3781 attaattcgc ctcctcattt cttctgctgt catccgcaca gcagaagaat ttctcattga
     3841 ctattatcgc gcaatttgct cacattgatt aaattaaacc acacactata agatataaac
     3901 ctctgcctac agctgtaaga aactccgctc agtactgaag tgccagtcct atttcctctt
     3961 ttctccagcc tgttatatta agcatactga ttaacgattt ttaacgttat ccgctaaata
     4021 aacatatttg aaatgtatgc gaccacagtg aaaaacaaaa tcacgcaaag agacaactat
     4081 aaagaaatca tgtctgtaat tgtggttgtc ttattactga cccttacgtt gatagccatt
     4141 ttttcggcaa ttgatcagct gggtatttca gaaatgggtc gcattgcaag agatcttaca
     4201 catttcatta tcaatagttt gcaagactga aaataaacag caaattataa atatgaaatg
     4261 ttaaaaaagt atcgataaaa actttattgt tttaaggaga taaaatgtcg ctcgtttgtt
     4321 ctgttatatt tattcatcat gccttcaacg ctaacatttt agataaagat tacgccttct
     4381 ctgacggcga gatcctgatg gtagataacg ctgttcgtac gcattttgaa ccttatgagc
     4441 ggcattttaa agagatcgga tttactgaaa ataccattaa aaaatatcta caatgcacta
     4501 acatccagac agtgacggtg cctgttcctg cgaagttttt acgtgcttca aatgtaccga
     4561 ctggattgct taatgaaatg attgcttatc ttaactcgga agaacgcaat catcataatt
     4621 tttcagaact tttgcttttt tcttgcctgt ctatttttgc cgcatgcaaa ggtttcatta
     4681 cactattaac taacggtgtg ctatccgttt ccggaaaagt gagaaatatt gtcaacatga
     4741 agctggcgca cccatggaag ctgaaagata tttgtgactg cctgtacatc agtgaaagcc
     4801 tgttgaagaa aaaactaaag caagagcaaa cgacattctc acagattcta ttagatgcca
     4861 gaatgcagca cgcaaaaaat ttgatacgcg tagaaggttc agtcaataaa attgccgaac
     4921 aatgtggtta tgccagtaca tcttatttta tttatgcgtt ccgcaaacat ttcggcaaca
     4981 gtccgaagag agtttctaag gagtaccgtt gtcaaagtca catgggtatg aatacgggca
     5041 acacgatgag tgctttagct atttgattat ttgctaacga gtagtcaacc acgcacgctg
     5101 cgtaagaatt aaatggggca gccattccct gccccgcgtt gtttttaggc gatatattta
     5161 ttgaaataaa taagtgacat ccatcacata tttatgcact tgcataacct gttgcagatt
     5221 atttatgatc tcaattcggc attttgtcag taaaatgcaa taatttatta aatatcaata
     5281 aattagttgt ttatcggcga gaaattactt aatagaacag aaagtaatgt caacgcttta
     5341 tggactgtta tttccctttt tttagctaaa tctgctatct ctttatgtga ctaacttcac
     5401 ttacatccac ttatttctct tcgtaaaatt acttcggaat taagtacaat aagaagagga
     5461 acatttatga agtctgcatt aaagaaaagt gtcgtaagta cctcgatatc tttgatactg
     5521 gcatctggta tggctgcatt tgctgctcat gcggcagatg atgtaaagct gaaagcaacc
     5581 aaaacaaacg ttgctttctc agattttacg ccgactgaat acagtaccaa aggaaagcca
     5641 aatattatcg tactaaccat ggatgatctt ggttatggac aacttccttt tgataaggga
     5701 tcttttgacc caaaaacaat ggaaaatcgt gaagttgtcg atacctacaa aatagggata
     5761 gataaagcca ttgaagctgc acaaaaatca acgccgacgc tcctttcatt aatggatgaa
     5821 ggcgtacgtt ttactaacgg ctatgtggca cacggtgttt ccggcccctc ccgtgccgca
     5881 ataatgaccg gtcgagctcc cgcccgcttt ggtgtctatt ccaataccga tgctcaggat
     5941 ggtattccgc taacagaaac tttcttgcct gaattattcc agaatcatgg ttattacact
     6001 gcagcagtag gtaaatggca cttgtcaaaa atcagtaatg tgccggtacc ggaagataaa
     6061 caaacgcgtg actatcatga caacttcacc acattttctg cggaagaatg gcaacctcaa
     6121 aaccgtggct ttgattactt tatgggattc cacgctgcag gaacggcata ttacaactcc
     6181 ccttcactgt tcaaaaatcg tgaacgtgtc cccgcaaaag gttatatcag cgatcagtta
     6241 accgatgagg caattggcgt tgttgatcgt gccaaaacac ttgaccagcc ttttatgctt
     6301 tacctggctt ataatgctcc gcacctacca aatgataatc ctgcaccgga tcaatatcag
     6361 aagcaattta ataccggtag tcaaacagca gataactact acgcttccgt ttattctgtt
     6421 gatcagggtg taaaacgcat tctcgaacaa ctgaagaaaa acggacagta tgacaataca
     6481 attattctct ttacctccga taatggtgcg gttatcgatg gtcctctgcc gctgaacggg
     6541 gcgcaaaaag gctataagag tcagacctat cctggcggta ctcacacccc aatgtttatg
     6601 tggtggaaag gaaaacttca acccggtaat tatgacaagc tgatttccgc aatggatttc
     6661 tacccaacag ctcttgatgc agccgatatc agcattccaa aagaccttaa gctggatggt
     6721 gtttccttgc tgccctggtt gcaagataag aaacaaggcg cgccacataa aaatctgacc
     6781 tggataacct cttattctca ctggtttgat gaggaaaata ttccattctg ggataattac
     6841 cacaaatttg ttcgccatca gtcagacgat tacccgcata accccaacac tgaggactta
     6901 agccaattct cttatacggt gagaaataac gattattcgc ttgtctatac agtagaaaac
     6961 aatcagttag gtctctacaa actgacggat ctacagcaaa aagataacct tgccgccgcc
     7021 aatccgcagg tcgttaaaga gatgcaaggc gtggtaagag agtttatcga cagcagccag
     7081 ccaccgctta gcgaggtaaa tcaggagaag tttaacaata tcaagaaagc actaagcgaa
     7141 gcgaaataac taaaccttca tgcggcggat ttttccgccg ccttattgag cgagatagcg
     7201 atgcacgtta cagccaagcc ctccagtttt caatgtaatc tcaaatgtga ttactgtttt
     7261 taccttgaaa aagagtcgca gtttactcat gaaaaatgga tggatgacag cactctgaaa
     7321 gagttcatca aacaatatat cgaagcgtct ggcaatcagg tctattttac ctggcaaggc
     7381 ggtgaaccca ctctggctgg cctggatttt ttccgtaaag ttattcacta tcaacaacgc
     7441 tatgcaggcc aaaaacgtat ttttaatgca ttacaaacga atggcatttt attgaataat
     7501 gaatggtgtg cctttctcaa agaacatgaa tttctggttg gtatctcgat cgatggcccc
     7561 ctggagttac atgaccgtta cagacgcagt aattcaggta acggtacttt tgcaaaagtg
     7621 atagcagcca tcgagcgtct gaaatcatat caagtagagt ttaatacgtt aaccgtcatt
     7681 aataacgtta atgtccatta ccctcttgag gtttatcatt ttttaaaatc tatcggcagt
     7741 aaacatatgc aatttatcga attgctagaa accgggacgc cgaatattga tttcagtggt
     7801 catagtgaga acacattccg tatcattgat ttttctgtgc ctcccacggc ttatggcaag
     7861 tttatgtcaa ccatttttat gcaatgggtt aaaaacgatg tgggtgaaat tttcattcgt
     7921 cagtttgaaa gctttgtcag ccgttttttg gggaatgggc atacaagttg tattttccag
     7981 gagtcctgca aggataatct ggttgttgaa agtaatggag acatttacga atgcgaccat
     8041 tttgtctatc cacagtacaa aattggaaac attaataaat ctgaactcaa aacgatgaac
     8101 agtgtacaac tgacagcgca aaaaaaacgg atttcagcga aatgtcagca atgtgcatat
     8161 aaacctatct gcaatggcgg ttgtcctaag catcgtatta ctaaagtaaa caatgagact
     8221 gtttcttatt tttgcgaagg ttataaaatc cttttttcaa ccatggtacc ttatatgaac
     8281 gccatggttg agttagctaa gaacagagta ccgctttacc acattatgga tgttgcaaaa
     8341 caaatggaga ataattaata acgctctctt ttaactccat tcttagcctc cttcttatga
     8401 ccttaacaag gcacccgaca taaattcctg tcgggtgttt cgtcatcatc atcttccctt
     8461 gcgaaaatcc tggcttacgt ggtatgcgtc agggcattag caataccctt gattatcaat
     8521 agcataacca taaccataac cactgaaaat taaaaatttc tatatatttc atattgttaa
     8581 atgatactta aataaatttc atcagtaaat cattgccata gataatcatt ggatggcaca
     8641 atcaattttc gctaatgata accattccca ttacgtttta tatgttgata gcaaagtatc
     8701 tctgtctgct gaagcccttt tggctgcgta agaataataa aacttcagtg ttgttaatca
     8761 ttattatcct cgcaatgatc cttggcgtgg ttaaaattca ggtctggtta aacgactgga
     8821 ataatgattt tttcaatgcg ctaagccaga aagaaaccga taagctttgg cagcttgttc
     8881 tttggttccc tgccctgctg gggatctttg tattaatatc cgtaaacaaa acatggttaa
     8941 ttaaactact gactattcgc tggcgcgaat ggttaacaga ttactacctt aatcgctggt
     9001 ttgcagataa aaactactac ttcacgcaga tctacggcga gcataaaaat acagataacc
     9061 ctgaccaacg tatcgctgaa gacattctct tactgatcag caaaacgctc agtctttcat
     9121 ttggcttcat ccagtcactt agcatgctga tcacctttac cgttattctt tggcaaagcg
     9181 cgggtacgct ctcattcact gttggtggaa cagaatggaa tatccagggg tatatggtct
     9241 ataccgtcgt gctcattgtg atcggtggaa ctttatttac ccataaggtg ggtaaacgta
     9301 ttcgtccgct taatgtggaa aaacaacgta gcgaagcgac atttcgaact aatcttgtgc
     9361 agcataataa gcagacagaa ctgattgcgc taagcaatgc tgagtctcta caacgccagg
     9421 agttgcgcga caattttcat accattaaag agaactggca tcgtttaatg aatcgtcagc
     9481 ggtggcttga ttactggcaa aatatttatt cgcgctcgct tagcgttctt ccctactttc
     9541 tgttattgcc gcagtttatt agcgggcaga tcaatctggg cggactgatg aaatcgcgcc
     9601 aggcatttat gctagtatcg aacaatttaa gctggtttat ttataaatat gacgaacttg
     9661 ctgaactggc tgcggttatc gatcgcttgt atgagttcca tcaactcact gaacagcgcc
     9721 ctacgaataa gcctaaaaat tgccaacatg cggtacaagt ggctaatgcg agtattcgta
     9781 cgcctgataa taagatcata ttagagaact tgaactttca tgtttcgcca ggcaaatggc
     9841 tattactgaa aggctactct ggtgcgggaa aaaccacact gcttaaaaca ttatcccact
     9901 gctggccgtg gtttaaaggt gatatttctt ctcctgctga cagttggtat gtgtcacaaa
     9961 caccgttaat caaaagcggc ttactgaaag agattatttg taaagcactt cccctgcccg
    10021 tagacgataa atcgttgagc gaagtactgc atcaggttgg tctggggaaa ttggctgcgc
    10081 gtattcatga ccacgatcgc tggggagata ttctttccag cggcgaaaaa caacgtatcg
    10141 ccctggcacg attaatttta cgacgtccga aatggatatt tcttgacgaa actacctctc
    10201 atcttgagga acaagaggct atacgcttac tgcgtttagt gcgtgaaaaa ctacccacaa
    10261 gcggcgtcat tatggttaca catcaacccg gtgtctggaa cctggctgat gatatttgtg
    10321 acattagcac ggttttataa caacaataaa agccctgctg ta
//
LOCUS       DAJTZL010000142        10340 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.142, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000142 DAJTZL010000000
VERSION     DAJTZL010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10340
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.142"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(41..1045)
                     /gene="cra"
                     /locus_tag="OO544_003165"
     CDS             complement(41..1045)
                     /gene="cra"
                     /locus_tag="OO544_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HCR6768693.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREVAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(1127..1213)
                     /locus_tag="OO544_003166"
     CDS             complement(1127..1213)
                     /locus_tag="OO544_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HCR6768694.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(1225..1716)
                     /gene="ilvN"
                     /locus_tag="OO544_003167"
     CDS             complement(1225..1716)
                     /gene="ilvN"
                     /locus_tag="OO544_003167"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCR6768695.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVASTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIREVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(1719..3443)
                     /gene="ilvI"
                     /locus_tag="OO544_003168"
     CDS             complement(1719..3443)
                     /gene="ilvI"
                     /locus_tag="OO544_003168"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HCR6768696.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     TAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTKGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVHNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            3562..3606
                     /locus_tag="OO544_003169"
     CDS             3562..3606
                     /locus_tag="OO544_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6768697.1"
                     /translation="MKRNDKILHYEGLN"
     gene            complement(3761..4705)
                     /gene="leuO"
                     /locus_tag="OO544_003170"
     CDS             complement(3761..4705)
                     /gene="leuO"
                     /locus_tag="OO544_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HCR6768698.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
     gene            5365..5451
                     /gene="leuL"
                     /locus_tag="OO544_003171"
     CDS             5365..5451
                     /gene="leuL"
                     /locus_tag="OO544_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HCR6768699.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            5544..7115
                     /gene="leuA"
                     /locus_tag="OO544_003172"
     CDS             5544..7115
                     /gene="leuA"
                     /locus_tag="OO544_003172"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HCR6768700.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSSAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            7115..8206
                     /gene="leuB"
                     /locus_tag="OO544_003173"
     CDS             7115..8206
                     /gene="leuB"
                     /locus_tag="OO544_003173"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HCR6768701.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLNAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKRF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAACAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            8209..9609
                     /gene="leuC"
                     /locus_tag="OO544_003174"
     CDS             8209..9609
                     /gene="leuC"
                     /locus_tag="OO544_003174"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HCR6768702.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFNGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKTGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVKRASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            9620..10225
                     /gene="leuD"
                     /locus_tag="OO544_003175"
     CDS             9620..10225
                     /gene="leuD"
                     /locus_tag="OO544_003175"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HCR6768703.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIAAYEAKQPAFMN"
BASE COUNT         2711 a         2717 c         2523 g         2389 t
ORIGIN      
        1 ccagagggaa attcacctgg cgcgtatttt tgttcgcggc ttagctacgg ctgagcacac
       61 cgcggcgata gagattacgt ttaatgcgcg ttaaaccagg ttttggctta cgcggttcgt
      121 ccaggcttgc caggacaatc tccagcacac gctctgcgac atcgcggtga cgttgagcca
      181 ctgccagcac cgggcactgt aagaagtcga gcagttcgtt atcgccaaag gtggcaattg
      241 ccaggtcaga aggcagttta ccatcgcgac gtagcgtgac atccatcacc ccttgcaaca
      301 acgcaaacga cgttgtgaac agcgcctgcg gcatcggatg cgtttccagc catttttcga
      361 ataactgggc agccacctcc cgctcatagc tgttggcata caggaaatgc acttcgcgcg
      421 gatcatcttt ccaggcagta cggaaacctt gttcacgcag gaagctgaca gaaagctccg
      481 gcagcgcgcc aagataaagc accgtctcgg cgggaaactt acgtaactct tccgccagca
      541 tttcggcatc atcctgatcg gcaccaacca cgctggtgaa gtgttcacga tcgagggcgc
      601 ggtccagcgc gacaatcggg aacgggtcgt tagcccagcg ttgataaaaa ggatgctcag
      661 gaggcaacga cgtcgaaaca ataatggcat cgacctgacg ctgtaaaagg tgctcaatac
      721 agcgcatttc gttgtctggc tgatcttctg agcaggcaat tagcagttga taaccccgtt
      781 gccgcgcctg gcgttcaaga tagttagcga tgcgggtata gctggtgttc tccagatcgg
      841 ggatcacaag accaatagaa cgtgtgcgtc cagcacgaag cccagctgcc acggcgttcg
      901 ggtggtaatt gtgctcacgc accacagcca tgactttttc aacggttttg tcgctcacac
      961 ggtattgctt cgctttgccg ttaataacat agcttgcagt ggtccgcgac actcccgcca
     1021 gccgagcgat ttcatccagt ttcacaattg ccccttgcgt aaaatgtaaa aaccatagcc
     1081 attgtactgg tatgggttaa attcattaac atctaagcgc ataaaattaa ctgcggcaac
     1141 tgcttttatt ccaggttccc actgatttcg caaaaaaagc ccaacgtgac atgttgggct
     1201 gcaaattgcg cattcagatc attctcaacg cattatttta tcgccgcgcg aaagtccgac
     1261 cacaccagaa cgagcaactt ccacaatttt cgccacctcg cgaatcgatg ctaaaaatgc
     1321 atcaagctta tcgctggtgc ctgctaattg aacggtataa agcgagggtg tgacatcgat
     1381 aatttgccca cggaatattt ccgtattacg tttcacttcg tcacgcccgt aaccgctggc
     1441 ctgaattttc accagcatga tttcccgctc aacatgcgcg ccctgcccca actcactcac
     1501 gcgcaagaca tcgaccagct tgtgtaattg cttttcgatc tgctcaagta ctttttcatc
     1561 gcccacggtc tggatggtca tacgcgataa tgtcggatca tcggttgacg caacggtcag
     1621 gctttcaatg ttgtagccac gctgggaaaa aaggccaatc acgcgggata acgcgcctga
     1681 ttcattttcg agtaagactg ataatatccg gcgcataatc aggttctctc cgttttgctt
     1741 aaccacattt catccattcc gcccccgcga atctgcatcg ggtagacgtg ctcgctgcca
     1801 tcgacggtaa catcaacaaa caccaggcga ttattgtgca cctgttccag cgcctcgcta
     1861 agtttgcttt ccagctcatg cggatgagaa atctggatcc cgacgtgacc ataggcttcc
     1921 gccagacgga cgaaatcggg tagcgattgc atataagatt gtgaatgacg gccggaatag
     1981 atcatgtcct gccactgctt caccatcccc agatagcggt tattgagatt caccaccagt
     2041 acgggcaact cgtattgcaa cgcggtagac agttcctgga tgttcatctg aatactgccg
     2101 tcgccagtga cgcaaaccac ggtttcttct ggcaacgcca ttttgacgcc cagtgccgca
     2161 ggtaaaccaa aacccatcgt gccgaggcca ccggaattga tccagcgacg cggtttgtcg
     2221 aatggataat aaagtgcagc aaacatctgg tgctgcccga catcggacgt cacgtaagcg
     2281 tctcccttcg tcaaccgcca aagagtctcg atcaccgcct gcggtttaat cttttcactg
     2341 tgagtgtcat atttcaggca ctgacgagcg cgccactgtt caatttgctg ccaccagtcg
     2401 cggatctcat ccagtggttg atgggcggat tcttgcgaca agagttcaag catttgttcg
     2461 aggacctggc gagcatcccc cacaatcggg atatccgcag tcacggtttt agaaatggaa
     2521 gtaggatcaa tatcgatatg cagaacagtg gcatttgggc agtactttgc cagattgttc
     2581 gtcgttcggt catcaaatcg taccccgacg gcgaaaatca catccgcgtt atgcatcgtc
     2641 atattggctt cgtaggtacc gtgcattccc agcatgccca gtgcctgacg atgcgttgcc
     2701 ggaaacgctc ccagccccat caatgaggaa acaacgggca gattcaacgc ctccaccgtt
     2761 tctttcaact gctgatggca gcccgccgtg attgccccac cgcctacgta gacaaccggt
     2821 ttttttgccg ctaccagcgt ttgcagagcg cgcttaattt gccctttatg tccggtagta
     2881 gtgggattgt aagaacgcat actgaccgac tccggccaga catagggtaa tttgttcgcc
     2941 ggattaagaa tatctttcgg taaatcaacg actactggtc caggacgacc acttgccgcc
     3001 agccagaaag cctttttcag cacctgcgga atgtcttccg tttgcttaac cagaaaactg
     3061 tgtttaacca ctggtcgcga aatccccacc atgtcgcact cctgaaaggc atcgtaacct
     3121 atcaacgagg tcgctacctg cccggaaagg acaactaatg gaatggaatc catataagcg
     3181 gtggcgatgc cagtaatcgc attggtcgcc cctggacccg acgttaccag cacgacgccg
     3241 acttccccgg tcgcgcgcgc cagaccatcg gccatatgca ctgccgcctg ctcatgacga
     3301 actaaaacat gatcgatacc acccacggta tgcaatgcat cgtaaatatc aaggactgcg
     3361 cctccgggat aaccgaatac ttgtttaacg ccctgatcga taagcgatcg gacgaccatc
     3421 tcggctccag acaacatctc catggcctgc ctcactgttt gacggaaaaa atgtgtaaaa
     3481 cacattaacc gtccgatctt gcttaagcaa ttggcagcca gagacataaa cgcagggtaa
     3541 taagaataaa caggaggaaa ggtgaaaaga aacgataaaa tcctccatta cgaaggatta
     3601 aattgataat aaatcggctg aatcccacaa cttacagcaa tttctctatt tttttaatta
     3661 atcctaaaat agagagccca cacattcaga ataaaattct acattcaata aaaaatacag
     3721 aataaaccag acattcatgt ctgacctatt ctgcattcag ttagcgtttg caaattgaga
     3781 ctaattgctc ttccatccac tgatggcctt tatcgcgccc ggcagcttca tgccaggaga
     3841 gataacaggt tctgctattt tgttttaacg gcagcggtaa tacctgtaat tctaaggatt
     3901 cagcgaactc ttcagccagc caacgcggcg caatagcgac caaatgcgtt tgcgacacca
     3961 cgctaagtac gctcatcatt gccatgccct gatacgcgat actggcttgc ttatctaccg
     4021 tgtcatacca aggttgacta aatgacgcga aacgatcgag cgaaaccgcc gcatgttgtt
     4081 cgttataaac atcatgtttc agtaacgggc ccttaattgt tggatgattt ttgctggcta
     4141 ccagcaccat ttcatcttta aataatggca cgctggtaaa ttcaggacga tggaagtctt
     4201 cataactaat cacaaactcc gtttcctgat aacgcagctg atgttcagtg ttctgattta
     4261 atgaagactt gaacataaca tgtatatttg gcgcaatctg ctcaatgtga ttataaatct
     4321 gcgaggtcag aatgctgtct aacgggctgc aaacacaaag atgaaataca cgttcactgc
     4381 tcgcgggttc aaaacctgaa ccaggcaatt cattttgtac tagttgcaat gcctgacgaa
     4441 ctgagccaaa aagttgaaat gcgcgagcag tcggttgaat accacggcca taacgaacaa
     4501 aaagctcgtc attaaacatc accttcaggc gtgcaacagc gttactgacc gcaggttgcg
     4561 acatacccag aacatgagcg gcacgggtaa tgttttgctc ctgcatcacg gcatcgaaaa
     4621 cggttaataa gttgagatcg accatgcgta gctgtggctt gcttaactcc gccgtctctg
     4681 aatgatctgt ttgtacctct ggcatactta actccactgt cacacttaac tccctttccc
     4741 ttattggaat gcagagaaat aatcctgaag aatatgattt atcatgcata taaaataaga
     4801 aaaagcaaaa tgagtaaaat tcggaaaaca taaagatacg ggttctgata aaaacagaat
     4861 ccattgccgt ggaatgagtc atttaccaca gcataagaat ccataatgtt tttgttgcga
     4921 aaacaatcta atcataacta ccgcgaatac tcaatcatct acaaaatgga ttaaatgtgc
     4981 ttatttaata attaatttat atatttaatg cattaattct taacattaat tgatcaataa
     5041 tattcaccaa atcaatatca aaaaaaatcg caaaacatat aattcaatac aaatcatcag
     5101 gataggtttt gcaacgcgtg cattttgtcc cctttttcct cgttgattag atgcaaaaat
     5161 ttatgctgaa atatgtcaac cgatgaaaag cgtcggtagt taagcagaaa ttaatatcgc
     5221 ttactttaac caccgcagca caattagcta attttacgga tgcagaactc acgctggcgg
     5281 gacgttttta ttgcgtcagg gttgacatcc gtttttgtat ccagtaactc taaaagcata
     5341 tcgcattcat ctggagctga tttaatgact cacatcgttc gctttatcgg tctactacta
     5401 ctaaacgcat cttctttgcg cggtagacga gtgagcggca tccagcatta agccagcacg
     5461 cagtcaaaca aaaaacccgc gccattgcgc gggttttttt atgcccgaag cgaggcgctc
     5521 taaaagagac aaggacctaa accatgagcc agcaagtcat tattttcgat accacattgc
     5581 gcgacggtga acaggcgtta caggcaagct tgagtgtgaa agaaaaactg caaattgcgc
     5641 tggcccttga gcgtatgggt gttgacgtga tggaagtcgg tttccccgtc tcttcgccgg
     5701 gcgattttga atcggtgcaa accatcgccc gccaggttaa aaacagccgc gtatgtgcgt
     5761 tagctcgctg cgtggaaaaa gatatcgacg tggcggccga atccctgaaa gtcgccgaag
     5821 ccttccgtat tcataccttt attgctacct ccccaatgca catcgccacc aagctgcgca
     5881 gcaccctgga cgaggtgatc gaacgcgcta tctatatggt gaaacgcgcc cgtaattaca
     5941 ccgatgatgt tgaattttct tgcgaagatg ccgggcgtac acccattgcc gatctggcgc
     6001 gagtggtcga agcggcgatt aatgccggtg ccaccaccat caacattccg gacaccgtgg
     6061 gctacaccat gccgtttgag ttcgccggaa tcatcagcgg cctgtatgaa cgcgtaccta
     6121 acatcgacaa agccattatt tccgtacata cccacgacga tttaggcctg gcggtcggca
     6181 actcactggc ggcggtacat gccggtgcac gccaggtgga aggtgcaatg aacgggatcg
     6241 gcgagcgtgc cggtaactgt tcgctggaag aagtcatcat ggcgattaaa gttcgtaagg
     6301 atattctcaa cgtccacacc gccattaatc accaggagat atggcgcacc agccagttag
     6361 ttagccagat ttgtaatatg ccgatcccgg caaacaaagc cattgttggc agcagcgcat
     6421 tcgctcactc ctccggtatc caccaggatg gcgtgctgaa aaaccgcgaa aactacgaaa
     6481 tcatgacacc agaatctatt ggtctgaacc aaatccagct gaatctgacc tctcgttcgg
     6541 ggcgtgcggc ggtgaaacat cgcatggatg agatggggta taaagaaagt gaatataatt
     6601 tagacaattt gtacgatgct ttcctgaagc tggcggacaa aaaaggtcag gtgtttgatt
     6661 acgatctgga ggcgctggcc ttcatcggta agcagcaaga agagccggag catttccgtc
     6721 tggattactt cagcgtgcag tctggctcta acgatatcgc caccgccgcc gtcaaactgg
     6781 cctgtggcga agaagtcaaa gcagaagccg ccaacggtaa cggtccggtc gatgccgtct
     6841 atcaggcaat taaccgcatc actgactata acgtcgaact ggtgaaatac agcctgaccg
     6901 ccaaaggtca cggtaaagat gcgctgggtc aggtggatat tgtcgccaac tacaacggtc
     6961 gccgcttcca cggcgtcggc ctggccaccg atattgtcga gtcctccgcc aaagccatgg
     7021 tgcacgtact gaacaatatc tggcgtgccg cagaagtcga aaaagagttg caacgcaaag
     7081 ctcaacacaa cgaaaacaac aaggaaaccg tgtgatgtcg aagaattacc atattgccgt
     7141 attgccgggg gacggtattg gtccggaagt gatgacccag gcgctgaaag tgctgaatgc
     7201 cgtgcgcaac cgctttgcga tgcgcatcac caccagccat tacgatgtag gcggcgcagc
     7261 cattgataac cacgggcaac cactgccgcc tgcgacggtt gaaggttgtg agcaagccga
     7321 tgccgtgctg tttggctcgg taggcggccc gaagtgggaa catttaccac cagaccagca
     7381 accagaacgc ggcgcgctgc tgcctctgcg taagcgcttc aaattattca gcaacctgcg
     7441 cccggcaaaa ctgtatcagg ggctggaagc attctgtccg ctgcgtgcag acattgccgc
     7501 taacggcttc gacatcctgt gcgtgcgcga actgaccggc ggcatctatt tcggtcagcc
     7561 aaaaggccgc gaaggtagcg gacaatatga aaaagcgttt gataccgagg tgtatcaccg
     7621 ttttgagatc gagcgtattg cccgcatcgc gtttgaatct gctcgtaaac gtcgccacaa
     7681 agtcacctca atcgacaaag ctaacgtgct gcaatcctct attttatggc gtgaaatcgt
     7741 caacgagatc gccacggaat acccggatgt cgaactggcg catatgtaca tcgacaacgc
     7801 caccatgcag ctgattaaag atccatcaca gtttgacgtt ctgctgtgct ccaacctgtt
     7861 tggcgacatt ctgtctgacg agtgcgcaat gatcactggc tcgatgggga tgttgccttc
     7921 cgccagcctg aacgagcaag gttttggtct gtatgaaccg gcaggcggct cagcaccaga
     7981 tatcgcaggc aaaaacatcg ccaacccgat tgcgcaaatt ctgtcgctgg cactgctgct
     8041 gcgctacagc ctggatgccg atgatgcggc ttgcgccatt gaacgcgcca ttaaccgcgc
     8101 attagaagaa ggcattcgca ccggggattt agcccgtggc gctgccgccg ttagtaccga
     8161 tgaaatgggc gatatcattg cccgctatgt ggcagaaggg gtgtaatcat ggctaaaacg
     8221 ttatacgaaa aattattcga cgctcacgta gtgtacgaag ccgaaaacga aaccccgctg
     8281 ttatatatcg accgccacct ggtgcatgaa gtgacctcac cgcaggcgtt caatggtctg
     8341 cgcgcccacg gtcgcccggt acgtcagccg ggcaaaacct tcgccactat ggatcacaac
     8401 gtctctaccc agacgaaaga cattaatgcc tgtggtgaaa tggcgcgcat ccagatgcag
     8461 gagctgatta aaaactgcaa agaatttggc gtcgaactgt atgacctgaa tcacccgtat
     8521 caggggatcg tccacgtaat ggggccggaa cagggcgtca ccttgccggg gatgaccatt
     8581 gtctgcggcg actcgcatac cgccacccac ggcgcgtttg gcgcactggc ctttggtatc
     8641 ggcacttccg aagttgaaca cgtactggca acgcaaaccc tgaaacaggg ccgcgcaaaa
     8701 accatgaaaa ttgaagtcca gggcaaagcc gcgccgggca ttaccgcaaa agatatcgtg
     8761 ctggcaatta tcggtaaaac cggtagcgca ggcggcaccg ggcatgtggt ggagttttgc
     8821 ggcgaagcaa tccgtgattt aagcatggaa ggtcgtatga ccctgtgcaa tatggcaatc
     8881 gaaatgggcg caaaaaccgg tctggttgca ccggacgaaa ccacctttaa ctatgtcaaa
     8941 ggccgtttgc atgcgccgaa aggcaaagat ttcgacgacg ccgttgccta ctggaaaacc
     9001 ctgcaaaccg acgaaggcgc aactttcgat accgttgtca ctctgcaagc agaagaaatt
     9061 tcaccgcagg tcacctgggg caccaatcca ggccaggtga tttccgtgaa cgacaatatt
     9121 cccgatccgg cttcgtttgc cgatccggtt aaacgcgcgt cggcagaaaa agcgctggcc
     9181 tatatggggc tgaaaccggg cattccgctg accgaagtgg ctatcgacaa agtgtttatc
     9241 ggttcctgta ccaactcgcg cattgaagat ttacgcgcgg cagcggagat cgccaaaggg
     9301 cgaaaagtcg cgccaggcgt gcaggcactg gtggttcccg gctctggccc ggtaaaagcc
     9361 caggcggaag cggaaggtct ggataaaatc tttattgaag ccggttttga atggcgcttg
     9421 cctggctgct caatgtgtct ggcgatgaac aacgaccgtc tgaatccggg cgaacgttgt
     9481 gcatccacca gcaaccgtaa ctttgaaggc cgccaggggc gcggcgggcg cacgcatctg
     9541 gtcagcccgg caatggctgc cgctgctgct gtgaccggac atttcgccga cattcgcaac
     9601 attaaataag gagcacacca tggcagagaa atttatcaaa cacacaggcc tggtggttcc
     9661 gctggatgcc gccaatgtcg ataccgatgc aatcatcccg aaacagtttt tgcagaaagt
     9721 gacccgtacg ggttttggcg cgcatctgtt taacgactgg cgttttctcg atgaaaaagg
     9781 ccaacagcca aacccggact tcgtgctgaa cttcccgcag tatcagggcg cttccatttt
     9841 gctggcacga gaaaacttcg gctgtggctc ctcgcgtgag cacgcgccct gggcattgac
     9901 cgactacggc tttaaagtgg tgattgcgcc gagttttgct gacatcttct acggcaatag
     9961 ctttaacaac cagctgctgc cggtgaaatt aagcgatgca gaagtggacg aactgtttgc
    10021 gctggtgaaa gccaatccgg ggatccattt cgacgtggat ctggaagcgc aagaggtgaa
    10081 agcgggagag aaaacctatc gctttaccat cgatgccttc cgccgccact gcatgatgaa
    10141 cggtctggac agtattgggc ttaccttgca gcacgacgac gccattgccg cttatgaagc
    10201 aaaacaacct gcgtttatga attaatcccc ttgcccggtc aaatgaccgg gctttccgct
    10261 atcgtccacg tcatcaaacg tctttaacct ttgcggttaa aaataatgcg acaacagaaa
    10321 taaccgcaat tacccagtag
//
LOCUS       DAJTZL010000143        10251 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.143, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000143 DAJTZL010000000
VERSION     DAJTZL010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10251
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.143"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            42..118
                     /locus_tag="OO544_003176"
     tRNA            42..118
                     /locus_tag="OO544_003176"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:76..78,aa:Pro,seq:tgg)
     gene            265..1500
                     /locus_tag="OO544_003177"
     CDS             265..1500
                     /locus_tag="OO544_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HCR6768704.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYVRHYIAASEPQNEVAFTWQGGEPTLLGLDFYRRAVKLQAKYGAGRKISN
                     SFQTNGVLLDDKWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAARDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLLVVNK"
     gene            complement(1584..1745)
                     /locus_tag="OO544_003178"
     CDS             complement(1584..1745)
                     /locus_tag="OO544_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6768705.1"
                     /translation="MEFSFSPKLLVVAVAAALPLMASAADTPSTATARKGFAGYDHPN
                     QYPQNAAIY"
     gene            complement(2407..3603)
                     /gene="hemY"
                     /locus_tag="OO544_003179"
     CDS             complement(2407..3603)
                     /gene="hemY"
                     /locus_tag="OO544_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HCR6768706.1"
                     /translation="MLKVLLLFVLLIAGVVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(3606..4835)
                     /gene="hemX"
                     /locus_tag="OO544_003180"
     CDS             complement(3606..4835)
                     /gene="hemX"
                     /locus_tag="OO544_003180"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCR6768707.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAAAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQATQLE
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPAPA
                     PAPAPQGDTPAAAPQGE"
     gene            complement(4857..5597)
                     /gene="hemD"
                     /locus_tag="OO544_003181"
     CDS             complement(4857..5597)
                     /gene="hemD"
                     /locus_tag="OO544_003181"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HCR6768708.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGQQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPNYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTTVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNAANDALLRALQ"
     gene            complement(5594..6535)
                     /gene="hemC"
                     /locus_tag="OO544_003182"
     CDS             complement(5594..6535)
                     /gene="hemC"
                     /locus_tag="OO544_003182"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HCR6768709.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQNAKQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            6922..9468
                     /gene="cyaA"
                     /locus_tag="OO544_003183"
     CDS             6922..9468
                     /gene="cyaA"
                     /locus_tag="OO544_003183"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HCR6768710.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDHDTPLLQQYFS"
     gene            complement(9508..9828)
                     /gene="cyaY"
                     /locus_tag="OO544_003184"
     CDS             complement(9508..9828)
                     /gene="cyaY"
                     /locus_tag="OO544_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HCR6768711.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
BASE COUNT         2339 a         2619 c         2679 g         2614 t
ORIGIN      
        1 gactttaaag aattgaacta aaaattcaaa aagcagtatt tcggcgagta gcgcagcttg
       61 gtagcgcaac tggtttggga ccagtgggtc ggaggttcga atcctctctc gccgaccaat
      121 tttgaacccc gcttcggcgg ggttttttgt tttctgtgca tttcgtcacc ctcccttcgc
      181 aataaacgcc cgtaataact cattgcccca cggtatgatt tcgcccttaa cgtattgaag
      241 gatgacttca ggcaaggagt gaccatgctg caacaggttc caacgcgtgc ttttcatgtg
      301 atggcgaaac cgagtggttc cgattgtaat ctgaactgtg actactgttt ttatctcgaa
      361 aaacaatccc tttaccgcga aaagccagtc acgcatatgg acgatgacac gctggaagcg
      421 tatgtccgtc actatatcgc tgccagcgaa ccgcaaaacg aagtggcttt tacctggcag
      481 ggcggcgaac caacgttact cgggctggat ttttaccgcc gtgccgtaaa gttacaggcg
      541 aaatacggtg ctggcaggaa gataagtaac agcttccaga ctaacggcgt gctgctcgat
      601 gataaatggt gtgcatttct ggcagaaaat cattttcttg ttgggttatc gctggacggt
      661 ccggctgaga tccacaatca atatcgcgtg accaaaggtg gcagaccaac gcataagctg
      721 gtgatgcgtg ccctgacgct gctgcaaaaa catcatgtcg actataacgt gctggtctgc
      781 gtcaaccgca ccagcgcgca gcaaccgttg caggtttatg attttttgtg cgatgcggga
      841 gtcgaattca tccagtttat tccggtggtc gagcgtctgg ctgatgaaac agctgcccgc
      901 gatggactga aattgcatgc gcctggtgat attcaggggg aactgacgga atggtcggtg
      961 cgccccgatg aatttggtga atttctggtg gcgatattcg accactggat caaacgcgac
     1021 gtcggcaaga ttttcgtgat gaatatcgaa tgggcgtttg ccaattttgt cggtgcgccg
     1081 ggtgcggttt gccatcatca gccaacctgt gggcgctcgg tgattgttga gcacaacggc
     1141 gacgtttacg cctgcgatca ctatgtttat ccgcaatatc ggctggggaa tatgcatcag
     1201 caaacaattg cagaaatgat cgattccccg caacagcagg tgtttggtga agataaattt
     1261 aagcaattac cggcgcagtg tcgcagttgt aacgtgttaa aagcgtgctg gggaggctgc
     1321 ccgaaacacc gcttcatgct cgatgccagc ggcaaaccgg ggctgaatta tttgtgtgcc
     1381 gggtatcagc gttatttccg ccatctaccg ccatatctta aagcaatggc tgatttgctg
     1441 gcgcacggtc gcccggccag tgacattatg caggcacatt tgctggtggt gaataagtaa
     1501 aaaagcggcc attttgtggc cgcctgcggt tgattgccgg atgcggcgta aacgccttat
     1561 ccggcctaca tgatcgtgca aattcaataa attgcagcgt tctgtggata ctggtttggg
     1621 tgatcgtatc cggcaaagcc tttgcgtgcg gtggcagttg acggggtatc tgctgcgctg
     1681 gccatgagag gaagagcggc ggcgacagca acaacaagaa gtttgggtga aaacgaaaat
     1741 tccatgcaaa atgctccggt ttcatgtcat caaaatgatg acgtaattaa gcattgataa
     1801 ttgagatccc tctccctgac aggatgatta cataaataat agtgacaaaa ataaattatt
     1861 tatttatcca gaaaatgaat tggaaaatca ggagagcgtt ttcaatccta cctctggcgc
     1921 agttgatatg taaggcgggt ttattatatt gcgttgatta ttaatgcttt tttagtttta
     1981 acggcaatta atatatgtgt tattaattga atgaatttta tcattcataa taagtatgtg
     2041 taggatcaag ctcaggttaa atattcactc aggaagcaaa gaggattaca gaattatctc
     2101 ataacaagtg ttaagggatg ttatttcccg attctctgtg gcataataaa cgggtagatg
     2161 ctcattccat ctcttatgtt cgccttagtg cctcataaac tccggaatga cgcagagccg
     2221 tttacggtgc ttatcgtcca ctgacagatg tcgcttatgc ctcatcagac accatggaca
     2281 caacgttgag tgaagcaccc actgttgtca tacagacctg ttttaacgcc tgctccgtaa
     2341 taagagcagg cgttttttta tgcatcagaa aggtcccgga ggtgcttgcc tccgggtgag
     2401 aaggaactac tgtggtgggt tgttctgcaa cgttaacatc aaaccgtcgc gacgcatcgc
     2461 tgcagcttct tccggcttgt gcagtctgtc cagcgcgtcg gcaagccatg cgtaatcgta
     2521 ggcgtccgga cgttgtttca gcgctgcgcg gaaggcgagc gatgcttcct gccattctcc
     2581 gtgcttcatc agtgactggc ccagtgtgct ccacaacagc gggcgatcgc cgacgttttt
     2641 gatctgctgg cgcagcactt tttccagctg ttccggattg ttggttttca gtcgaggaat
     2701 cggcagcagt aggcgatcgt cgtactggcg tttcaggcca tcgatgataa tttgctgggc
     2761 agtatcatga tcgtcacatt caataagatg ttccgccatt gccacctgca acgctacctg
     2821 atgacgcgtt ttccggcttt ggtttttcca ccagttacgc aaaccttcac taccgttatc
     2881 ggcacgcgcc tgatccatca ggccaatcca tgcctgttgt tccagcattg cacgatgttc
     2941 ttcatcacca acatgggctt tcgccattga tgggatgata tccagcagag aactccatgc
     3001 acctgtgcgg atatacgcct gttccgccag acgtaatact tccggatggc gtggcgtaac
     3061 ttccagcagc ttatccacgc cgtggcgtgc agcatggttt tcattacggg ccagttgcag
     3121 acgtacacgg gtgatttcta ccggaatggt gtcattgccg gccagctctg ctgcgcgttc
     3181 cagatgttgg ttggcgcgtg cttcatcacc acgttgttgc gcggcttcgg cagccagtag
     3241 atagttcacc accggttgtt ccgcgtgatc ggcatttttc gccatcagct tttcaacttg
     3301 ctgataatcg ccttccgcca gtttcagcag cgcctgttcg gtctgcttac gggcacggcg
     3361 acgcttacgt ccgacaaacc acccacgggt gtgtgcgcct gtgcggaaga tccgccgcag
     3421 tagccactca atggcaaaca gcactaccat cgccagaatc aaaataatcg ccaggcccgt
     3481 gacgctggtt tcgatattgt agttgtcagt ctggatcagc acgtaaccct gatgaccggc
     3541 aatcatcggg ccaaccacta cccccgcaat cagcaaaaca aagagtaata acacttttag
     3601 catggttatt ctccttgcgg cgcggctgcc ggagtatcac cttgcggtgc aggtgcaggt
     3661 gcaggtgcag gtgcaggtgc aggtgcaggt gcaggtgcag gtgcaggttt agcttccgtt
     3721 gtccccgctg ccggttgtgc cagcaggtta cgcacgcggg tctgcatcag tttttccagc
     3781 atcgcctggc tttgcagggt ttccggaaga tccatcgaga tattttgctg gcttaactgg
     3841 tccacctcgt cgaggaacgc tttggtggtg gcatcatcag tatcgtagta agcacgtacc
     3901 caggtggaga cgttctccag cgcctggcga taagtctctt cctgatgacg cggtacagct
     3961 tgtgctgcga ccagcaggcg agagcgaata ttttcgcgca gatagatatc ctgatttggc
     4021 gctaacagcg gtacggcggt gtcatcacga cggcgaatcg taatgaagtt gtccataaag
     4081 ttctgccagc ttttttgcag attgatacgc cattcgctga tggaactgga aagctcttca
     4141 ccgtctgaat ccatcggcga accatcgctg tcattatcgg ccagacgcag gttatctacc
     4201 tgatttgaaa gctgattaag cttaaggatg atgccgtcat aatccacctg cgatactgca
     4261 gaaaggctgg cgatatcatc ggtaattgcc cgacgaacgg taatcagact cgggtcattc
     4321 atatccgcca ggctggcgtc tgcacttttc agcaacgctg cagcggtcgt gacgtcctga
     4381 tcgctccaca gcttccgtcc ggcgagtttc accagaaaat cggcctgggc cagcagccag
     4441 gttttagcat cgctgccgga aatggtggcg accttttgct ggacttcatc cagctgtttt
     4501 gccagcgttt cttgctgacg attcgcctgt tcaagctgcg tagcctgttg cttaataatg
     4561 ccttccagtt cggctttttg gctctcctgg gctttttgca atgccgtcag ttggttagcc
     4621 agggcatcgc tggtggcggt ctgattgacg gcctgttgtt taccccagcc atacaaaccg
     4681 acgcccgccg ccagagcaat agcgatagcc gccgcgctga gaatcaatgc ggtattgttc
     4741 ttactctttt tttctgttgc gacaggttgt gacgtggtgt ccacggcctc cctagtctct
     4801 tcaaccacgg cggaggtttt ttcttgttcc gtcattatgg cttcctgtta tgagagttat
     4861 tgtaatgccc gtaaaagcgc atcgttggca gcgttatcgg cgaccttaat gtcttgccag
     4921 cccagttccc gggcgagttt cgccaaacgc tcactgacga ccaatagtcg acagtgtagt
     4981 aaccagtgct cacgatacca ttgtgggatc agcgaccaga gttgctgcaa catttcaccg
     5041 ctggtaacaa cgaccgtcgt cacctcgcgg gattgccagc gcatcgcttc ttctgcacca
     5101 tcgtaatgga ttgcgcatcg ttgataacat tcacaaaaag tgacctcagc accgcgcgcc
     5161 gtcagggtat ccccaattag ctcacgaccg ccattgccac gtaatatcag cgcacgtttg
     5221 cccgcaatat tttgtaattc aggtaattgt agcaagactt cgctgatttc ccgatcctgc
     5281 gggtagagaa tcttctgtcc gcttacggta tgtagtgcca gtgcggtggt gcgtccaatg
     5341 gcgaaataat taggtagtcg gggccattta cgatcttgct gatgtagctg tgattgggca
     5401 aaagcaaccg cgtgttgcga gagggcaaac aacagatcgc tctcccccag cgctgccagt
     5461 tgatcagcaa gttgcggtaa ttgttgaccc ggagaaaact caatcagcgg aaaatgccag
     5521 gccacctgcc ccagtgtgcg cagacggctc actaactctt ctccagcggg agacgggcgg
     5581 gtgacaagga tactcatgcc ggggcgtctc cgttatagac ttcagcgagg atctcgcgcg
     5641 cgccgttatt cagtagctct tctgccagcg aaatccccat ttgtttggca ttttgcggcg
     5701 caccgcggcg ttcaccgcga ataatctgcg aaccgtccgg cgcgccgacc agcgcacgca
     5761 gccagatttc gccatcaata agctcggcgt agctaccaat tggcacctga catccgcctt
     5821 cgagacgggt attcatggcg cgttctgcgg taacgcgcag tgcagtttcg tggtgattca
     5881 gcgcggcaag cagctcgcga gtgcgcgtat catcaaggcg gcattcaata ccgactgcac
     5941 cttgtcctac cgccggaaga gaaatctcgg gtggtaacgc ggcgcgaatg cgggactcca
     6001 gacctaaacg ttttagtccg gctacggcaa gaatgatggc atcgtattcg ccgttatcta
     6061 gtttgctcag gcgagtgccg acgttgccgc gcagggagcg gataatcaga tccgggcggc
     6121 gttcagccag ttggcactgg cgacgtaaac tggacgtccc gacgatactg cctgctggta
     6181 acgcatccag actgtcatag ttattggaca caaaggcatc gcgaggatct tcacgctcac
     6241 aaatagtgac cagtcccaga ccttgcggga attcaaccgg cacatctttc attgaatgta
     6301 cggcgatatc ggcgcgattt tcgaggagcg cgacttccag ctcttttaca aataagcctt
     6361 ttccgcctac tttcgccagc ggcgtatcaa gaatcacatc gccgcgcgtc accatcggta
     6421 ccagttcaac gaccaggccc ggatggctcg ccatcaactt gtctttgaca tagtgtgcct
     6481 gccagagtgc aagtgggctt tggcgtgtgg caattcttaa aacattgtct aacatgcttg
     6541 ttaccgtcat tatcatccgt ggtccatcct aacatccttg ccagagtgat gtcagtgttg
     6601 tggtgaaacg tagacgcctg cgcaaaccgt aaaatgaggt ctggcagtgg atcctgacag
     6661 acgtttcacg ctgtcgtaat aaggaattta cagagaataa acggtgctac acttgtatgt
     6721 agcgcatctt tctttacggt caatcagcaa ggtgttaaat tgatcacgtt ttagaccatt
     6781 ttttcgtcgt gaaactaaaa aaaccaggcg cgaaaagtgg taacggttac ctttgacata
     6841 cgaaatatcc cgaatgccgc gtgttaccgt tgatgttggc ggaatcacag tcatgacggg
     6901 tagcaaatca ggcgatacgt cttgtacctc tatattgaga ctctgaaaca gagactggat
     6961 gccataaatc aattgcgtgt ggatcgcgcg cttgctgcta tggggcctgc attccaacag
     7021 gtctacagtc tactgccgac attgttgcac tatcaccatc cgctaatgcc gggttacctt
     7081 gatggtaacg ttcccaaagg catttgcctt tacacgcctg atgaaactca acgccactac
     7141 ctgaacgagc ttgaactgta tcgtggaatg tcagtacagg atccaccgaa aggtgagctt
     7201 ccaattactg gtgtatacac catgggcagc acctcgtccg tagggcaaag ttgttcctct
     7261 gacctggata tctgggtctg tcatcaatcc tggctcgata gcgaagagcg ccagttacta
     7321 caacgtaaat gcagcctgct ggaaagctgg gccgcctcgc tgggtgtgga agtcagcttc
     7381 ttcctgattg atgaaaaccg ctttcgtcat aatgaaagcg gcagcctggg gggcgaagat
     7441 tgtggctcca cccagcatat actgctgctt gacgaatttt atcgtaccgc cgtgcgtctc
     7501 gccggtaagc gtattctgtg gaatatggtg ccgtgcgacg aagaagagca ttacgacgac
     7561 tatgtgatga cgctttacgc gcagggcgtg ctgacgccaa atgaatggct ggatctcggt
     7621 ggcttaagct cgctttctgc tgaagagtac tttggtgcca gcctttggca gctctacaag
     7681 agtatcgatt ccccatacaa agcggtgctg aaaacactgc tgctggaagc ctattcctgg
     7741 gaatacccga acccacgtct gctggcgaaa gatatcaaac agcgtttgca cgacggcgag
     7801 attgtatcgt ttggcctcga tccatactgc atgatgctgg agcgtgttac tgaatacctg
     7861 acggcgattg aagatttcac ccgtctggat ttagtacgtc gctgcttcta tttaaaagtg
     7921 tgcgaaaaac tcagccgtga acgcgcctgt gtaggctggc gtcgcgcagt gttgagccag
     7981 ttagtgagcg agtggggttg ggacgaagct cgtctggcaa tgctcgataa ccgtgctaac
     8041 tggaagattg atcaggtgcg tgaggcgcac aacgagttgc tcgacgcgat gatgcagagc
     8101 taccgtaatc tgatccgctt tgcgcgtcgc aataacctta gcgtctccgc cagtccgcag
     8161 gatatcggcg tgctgacgcg taagctgtat gccgcgtttg aagcattacc aggtaaagtg
     8221 acgctggtaa acccgcagat ttcacccgat ctctcggaac cgaatctgac ctttatttat
     8281 gtgccgccgg gccgggctaa ccgttcaggt tggtatctgt ataaccgcgc gccaaatatt
     8341 gagtcgatca tcagccatca gccgctggaa tataaccgtt acctgaataa actggtggcg
     8401 tgggcatggt ttaacggtct gctgacctcg cgcacccgtt tgtatattaa aggtaacggc
     8461 attgtcgatt tgcctaagtt gcaggagatg gtcgccgacg tgtcgcacca tttcccgctg
     8521 cgcttacctg caccgacacc gaaggcgctc tacagcccgt gtgagatccg ccatctggcg
     8581 attatcgtta acctggaata tgacccgaca gcggcgttcc gcaatcaggt ggtgcatttt
     8641 gatttccgta agctggatgt cttcagcttt ggcgagaatc aaaattgcct ggtaggtagc
     8701 gttgacctgc tgtaccgcaa ctcgtggaac gaagtgcgta cgctgcactt caacggcgag
     8761 caatcgatga tcgaagccct gaaaactatt ctcggcaaaa tgcatcagga cgccgcaccg
     8821 ccagatagcg tggaagtctt ctgttatagc cagcatctgc gcgggttaat ccgtactcgc
     8881 gtgcagcaac tggtttctga gtgtattgaa ctgcgccttt ccagcacccg ccaggaaacc
     8941 gggcgtttca aggcgctgcg cgtttctggt caaacctggg gcttgttctt cgaacgcctg
     9001 aatgtatcgg tacagaagct ggagaacgcc atcgagtttt atggcgctat ttcgcataac
     9061 aaactgcacg gcctgtcagt gcaggttgaa accaatcacg tcaaattacc ggcggtggtg
     9121 gacggctttg ctagtgaagg gatcatccag ttcttcttcg aagaaacgca agacgagaat
     9181 ggctttaata tctacattct tgacgaaagc aaccgggttg aggtgtatca ccactgcgaa
     9241 ggcagcaaag aggaactggt gcgtgacgtc agtcgcttct actcgtcatc gcacgaccgc
     9301 ttcacctacg gctcaagctt catcaacttc aacctgccgc agttctatca gattgtgaag
     9361 gttgatggtc gtgaacaggt gattccgttc cgcacaaaat ctatcggtaa catgccgcct
     9421 gccaatcagg atcacgatac gccgctatta cagcaatatt tttcgtgatg aacgtgccgg
     9481 aaagcgaggc ttatccggca tgcaatctta gcggaaactg actgtttcac ccgcctgctg
     9541 cgtcgccgcc tgttccagca aatcccagaa ggtttcgccg ctgcgatcgc aaatccactc
     9601 atcgcctttc agatcaaaat ggtagccgcc ttgtttggtt gccagccata cctggtgcag
     9661 cggttcctgg cggttgataa tgattttgct gccgttttca aaggtaatgg tcagcacgcc
     9721 gccgttgatt tcgcagtcga tatcgctgtc gccatcccag tcgtccaggc gttcttcaat
     9781 ggtcagccag agttgatcag ccaggcgatg aaattcactg tcgttcattg ttgtatcctg
     9841 tttttaagtg atggcggcag tatagcggca tggggtcagg gcttcaaagt ttgcacctct
     9901 gcggctgcgt tccggcacga ttcatccgtc accggaataa tgatgtctct gtgtagcgaa
     9961 agatttgtct cttcattagg gcgcagttac accacgcctt tccctgtttc tggtaaacat
    10021 tatgatcagg tttaccgagc gagcatcctc acgctgacgg aactaaaaaa gacaacaaac
    10081 aaaccacatt gcgatagtgc atagagccat cctggcgcga ggtgccgatc acgaaactac
    10141 cagcaaaaca taaatcccca cgagtaagcg ttatactcgc agcatttcct cacttttcag
    10201 acttcataaa gagtcgctaa acgcttgctt ttacgtcttc tcctgcgatg a
//
LOCUS       DAJTZL010000144        10189 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.144, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000144 DAJTZL010000000
VERSION     DAJTZL010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10189
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.144"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..921
                     /locus_tag="OO544_003185"
     CDS             <1..921
                     /locus_tag="OO544_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6768712.1"
                     /translation="SATLSSTNWQYACSCSAGKAVKLVYMVSPVLTTTGHQTGYYKLN
                     DSLDIKTTLQANDIPGLTTDQVVSVNTRFTQIKSSTVYSAATQTGVCQGDTSRYGPVN
                     IGANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNLT
                     APQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQM
                     RIDGTTGVVDQYNLVARRRSSDNVPDVGIRIENLGGGVANIPFQNGILPVDPSGHGTV
                     NMRAWPVNLVGGELETGKFQGTATITVMVR"
     gene            complement(964..1824)
                     /gene="rsmI"
                     /locus_tag="OO544_003186"
     CDS             complement(964..1824)
                     /gene="rsmI"
                     /locus_tag="OO544_003186"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HCR6768713.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            1888..3924
                     /gene="lpoA"
                     /locus_tag="OO544_003187"
     CDS             1888..3924
                     /gene="lpoA"
                     /locus_tag="OO544_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HCR6768714.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAVEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKKQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTVDGVASPAQASVSDLTGDQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGTPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGAML
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPVS"
     gene            3882..4277
                     /locus_tag="OO544_003188"
     CDS             3882..4277
                     /locus_tag="OO544_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HCR6768715.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGRTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            4297..4887
                     /gene="diaA"
                     /locus_tag="OO544_003189"
     CDS             4297..4887
                     /gene="diaA"
                     /locus_tag="OO544_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HCR6768716.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            4897..5472
                     /gene="yraP"
                     /locus_tag="OO544_003190"
     CDS             4897..5472
                     /gene="yraP"
                     /locus_tag="OO544_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HCR6768717.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(5586..6626)
                     /locus_tag="OO544_003191"
     CDS             complement(5586..6626)
                     /locus_tag="OO544_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCR6768718.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQSGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(6699..7334)
                     /locus_tag="OO544_003192"
     CDS             complement(6699..7334)
                     /locus_tag="OO544_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HCR6768719.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTEPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            7462..7980
                     /gene="yhbO"
                     /locus_tag="OO544_003193"
     CDS             7462..7980
                     /gene="yhbO"
                     /locus_tag="OO544_003193"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HCR6768720.1"
                     /translation="MSKKIAVLISDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(7960..8403)
                     /locus_tag="OO544_003194"
     CDS             complement(7960..8403)
                     /locus_tag="OO544_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HCR6768721.1"
                     /translation="METLITISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARQAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            8454..8756
                     /gene="yhbQ"
                     /locus_tag="OO544_003195"
     CDS             8454..8756
                     /gene="yhbQ"
                     /locus_tag="OO544_003195"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HCR6768722.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(8743..9246)
                     /locus_tag="OO544_003196"
     CDS             complement(8743..9246)
                     /locus_tag="OO544_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR6768723.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(9240..9764)
                     /locus_tag="OO544_003197"
     CDS             complement(9240..9764)
                     /locus_tag="OO544_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HCR6768724.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            9973..>10189
                     /locus_tag="OO544_003198"
     CDS             9973..>10189
                     /locus_tag="OO544_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCR6768725.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPD"
BASE COUNT         2542 a         2815 c         2563 g         2269 t
ORIGIN      
        1 tctgcaacgc taagtagcac taactggcaa tacgcctgtt cctgctctgc gggtaaggca
       61 gttaaacttg tctatatggt cagccccgta cttaccacca ctggacatca gacaggatat
      121 tacaaactca atgacagcct ggatattaaa accacattac aggcaaacga cattccagga
      181 ctcacaaccg accaggttgt ctctgttaac acccgattca cacagataaa aagcagcacc
      241 gtatattctg ctgcaaccca aacgggtgtt tgccagggtg acacgtctcg ttatggaccc
      301 gttaatattg gtgcaaatac cacctttacc ctgtatgtca ccaagccatt tctcggctcg
      361 atgaccattc cgaaaacgga tattgccgtc attaaaggcg cgtgggtcga tggaatggga
      421 agcccgtcta caggtgactt ccatgattta gtcaagttat cgattcaggg aaatctcacc
      481 gccccacagt cgtgcaaaat taatcagggc gatgttatta aggttaattt tggattcatc
      541 aatggtcaga agtttaccac ccgcaatgcc atgccagacg gttttactcc agtagacttt
      601 gatatcactt atgactgtgg tgatacttca aagattaaaa actcgttgca aatgcgcatc
      661 gacggtacaa ctggggtagt agaccagtac aacctggtcg ccaggcgaag aagttcagac
      721 aatgtgcccg atgtcggtat tcgtattgaa aatctcggcg gcggagttgc aaatattcct
      781 tttcagaacg gtatccttcc cgttgatcct tccgggcatg gcaccgtcaa tatgcgcgcc
      841 tggccagtta atctggtcgg tggtgagctg gaaacaggaa aatttcaggg caccgccacc
      901 attaccgtca tggtgcggta aaagaagacg ttgcgttttg attaacgcaa cgcttcggca
      961 ctgttacccc tgctgctcca gcgcatactt atacagcgca tttttcttca cgccatgaat
     1021 ttctgcggcc agcgccgccg cttttttcag cggcagttct gcctgtagca gcgccagcgt
     1081 gcgcagggca tcggcgggta agtcttcttc ctgtgcttta tgaccttcga caatcagcac
     1141 catttcgcct ttgcgacggt tttcatcttc ctttacccac gccagcagct cgccaacggg
     1201 cgcgccgtga atggtttccc aggttttggt cagctcacgc gccagaacca cgtagcggga
     1261 ttcgcctaat accgcaacga tatcttccag gctatctaac agacggtggg tagattcata
     1321 aaaaatcagc gtgcgcggct ccgcttcaat ggcttttagc gcatcacggc ggccttttga
     1381 tttggcaggt aaaaagcctt cgtaacagaa acggtcagag ggtaaacccg cggcgcttaa
     1441 cgcagtgata gcagcacacg gtccgggtag cggcaccaca cgaatccccg cttcacggca
     1501 ggtacgcacc agatggtagc caggatcgtt aattagcggc gttccggcat cggaaaccag
     1561 cgcaatgttt tgcccctctt gcagcttcgc cagcagcgtt tcggcttttt gttgttcgtt
     1621 atggtcgtgc agcgcaaaca accgggcatt aatcccaaaa tgttgcagca ataaaccggt
     1681 gtgacgagta tcctcggcgg caatcagatc aacggcctgt aatacctcta acgcacgctg
     1741 ggtgatatcc gccagattgc cgattggcgt cggtacaatg taaagctggc cctgagaatt
     1801 atccgccgat tggtgttgtt tcattgtgtc gtccgtattg ccgatttaat attgagcatt
     1861 gcgtaaaaaa atatcactgg atacattatg gtaccctcaa cattttctcg tttgaaagcc
     1921 gcgcgttgtc tgcctgttgt tctggcagcc ctgattttcg ccggttgtgg cacccatact
     1981 cccgatcagt ccactgctta tatgcagggc acggcgcagg ctgattctgc cttttatctt
     2041 cagcagatgc agcaaagctc tgatgatacc aggatcaact ggcaattact cgccattcgt
     2101 gcactggtga aagaaggtaa aaccgggcag gcggttgagt tgtttaacca actaccgcaa
     2161 gaactgaacg attctcagcg tcgcgagaaa acactgctgg cggtagagat taaactggcg
     2221 cagaaagatt ttgctggcgc gcaaaacttg ctggcgaaaa tcacacctgc cgatttagaa
     2281 caaaaccagc aagcgcgtta ctggcaggca aaaatcgatg ccagccaggg gcgtccttcc
     2341 attgatttac tgcgcgcgtt aattgctcag gaaccgctgc tcggcgcgaa agaaaaaaag
     2401 cagaatattg atgccacctg gcaggcgctc tcctccatga ctcaggaaca ggcgaatacg
     2461 ctggtgatca acgccgacga aaatattctg caaggctggc tggatctgca gcgcgtctgg
     2521 tttgataacc gtaacgatcc cgacatgatg aaagccggga tcgccgactg gcaaaaacgt
     2581 tatccgaaca atccgggcgc gaaaatgctg ccaacgcagt tggttaacgt aaaagcgttt
     2641 aaaccagcct cgaccaacaa aatcgccctg ctgttgccgc tgaatggcca ggcagcggta
     2701 tttggtcgca ccattcagca aggctttgaa gcggcgaaaa atatcggcac tcagccagtg
     2761 gcggctcagg tagctgccgc acctgccgca gacgtagctg aacaacctca gccgcaaacc
     2821 gtggatggcg ttgccagccc ggcacaagcc tcggttagcg atctgaccgg tgatcagcct
     2881 gcggcccagc cggtgcctgt aagcgccccg gcgacaagca ccgcagcggt aagcgcaccc
     2941 gcaaatccat ccgcagagct gaaaatctac gatacctcat cacaaccact tagccagatc
     3001 ttaagccagg ttcagcagga tggcgcgagt attgtggttg gtccgttgct gaaaaataac
     3061 gttgaagagt tgctgaagag caacaccccg ctgaacgtgc tggcactgaa ccagccagag
     3121 aatatcgaaa accgcgtcaa tatttgttac ttcgcgcttt cacctgaaga cgaagcgcgc
     3181 gatgcagcgc gtcatattcg tgaccagggt aaacaagcgc cgctggtgct gatcccacgc
     3241 agttcattgg gcgatcgcgt agccaatgcg tttgcgcaag agtggcagaa actgggcggc
     3301 ggcaccgttc tgcaacaaaa atttggttcc accagcgaat tacgcgcggg tgttaacggc
     3361 ggttctggta tcgctttaac gggtaccccg attactccca gagcgacaac cgactccggc
     3421 atgacgacca acaatccaac gctgcaaacc acgccaaccg atgaccagtt caccaataat
     3481 ggcggtcgtg tcgatgcggt gtacattgtg gcaacgccgg gtgaaatcgc ttttatcaaa
     3541 ccgatgatcg ccatgcgtaa cggtagccag agcggtgcaa tgctgtacgc cagctcccgc
     3601 agcgcacaag gcaccgcagg cccggatttc cgtctggaga tggaaggttt gcagtacagc
     3661 gaaatcccga tgctggcggg cggtaatctg ccgttaatgc agcaggcact cagcgcggtg
     3721 aataacgatt attcactggc tcgcatgtat gcgatgggcg tcgatgcctg gtcgctggca
     3781 aatcatttct cacaaatgcg ccaggttcag ggttttgaaa tcaacggtaa taccggaagc
     3841 ctgacggcaa acccggattg cgtgattaac aggaagttat catggctaca gtaccaacaa
     3901 ggtcaggtag tccccgtcag ttaaccacca aacagaccgg cgatgcgtgg gaagcacaag
     3961 cgcgtcgctg gctggaaggc aaaggactgc ggtttatcgc cgctaacgtg aacgagcgtg
     4021 gcggcgagat cgatctgata atgcgtgaag gccggaccac cgtttttgtc gaggtacgct
     4081 atcgccgctc tgcgctttat ggcggcgcgg cagccagtgt gacccgcagc aaacaacaca
     4141 aattattaca gactgcccgc ttgtggctcg cgcgtcataa tgggagtttt gatactgtgg
     4201 attgccggtt cgatgtggta gccttcaccg ggaatgaggt tgagtggatt aaggatgcct
     4261 ttaatgacca ctcataatta aggtttaagg attagcgtgc aagaaagaat taaagcttgc
     4321 ttcactgaaa gcattcaaac tcaaattgcg gcggcagagg cgcttccgga tgccatctcc
     4381 cgtgcagcca tgacgctggt tcagtctctg ctcaatggca acaaaatcct ctgttgtggt
     4441 aatggaactt ccgctgccaa tgcacagcat tttgctgcca gcatgatcaa ccgtttcgaa
     4501 acggagcggc ccagcttacc tgccattgca ctaaatactg ataatgttgt cttaacggcg
     4561 attgccaacg atcgcttaca tgatgaagtg tatgcaaaac aggtgcgggc gctgggtcat
     4621 gcgggagatg tattgttagc catttccacc cgtggcaaca gccgcgatat tgttaaagca
     4681 gttgaagccg ccgttacgcg tgatatgacc attgtggcat tgaccggcta tgacggcggc
     4741 gagcttgcag gtttgttagg gccacaggat gtggagatcc gcattccttc gcatcgtagt
     4801 gctcgcattc aggaaatgca tatgctgacg gtaaattgcc tgtgcgatct gatcgataac
     4861 acgcttttcc ctcaccagga tgattaagga gaatacatga aggcattatc gccaatcgca
     4921 gtccttattt ccgcgctgct gttgcaaggt tgtgttgccg ctgccgtagt gggtaccgct
     4981 gctgtgggta ccaaagccgc aactgaccca cgcagtgtcg gcacccaggt ggacgatggt
     5041 accctggaag tgcgcgtgaa cagcgcattg tcgaaagacg aacagattaa gaaagaaacc
     5101 cgtattaatg tcacggcgta tcagggcaaa gtgctgctgg ttgggcagtc accaaacgct
     5161 gaactttcgg ctcgcgccaa acagattgct atgggcgtag acggtgccaa cgaagtgtat
     5221 aacgagattc gtcagggcca gccgattggt ctgggcgaag catctaacga tacgtggatc
     5281 accaccaaag tgcgttcgca gctcttaacc agcgacctgg tgaaatcgtc caacgtgaaa
     5341 gtgaccaccg aaaacggtga agtgttcctg atggggctgg tgactgaacg tgaagcgaaa
     5401 gcggcggcag atattgccag ccgggtgagc ggcgtaaagc gggtaaccac agcgtttacg
     5461 tttattaaat agcagcccct tgtaatgcct gatgcgacgc ttgccgcgtc ttatcaggcc
     5521 tacaacgatg caaaccgtag gtcggataag gcgtttacgc cgcatccgac atgttttccc
     5581 tcaaatcaga ataacagcgc cagcccgccg acaatcacgc ctgacactgc caccatcgcc
     5641 cccgtcagcc ataaggcttt cgccgggaac gctttgcgca gcataatcag tgacggcaaa
     5701 ctcactgccg ggagcgtcat caacaatgcc agcgccggag cggttcccat acctgccagc
     5761 atcatcgttt gtacaatcgg aatttctgct gccgtgggaa tgacaaacaa gcatcctgct
     5821 accgccatcg ccaccaccca catcaggctg ttatcgacag caccatcggc atgggggaat
     5881 aaccagacgc gtgcggcacc caacaccagt actgcaagga tgtaaaccgg gatcgtactc
     5941 cagaaaagtg tccatagcgc cctgccccag cggctaaaaa aaccgctctg tgcttccggt
     6001 atgtcaattt cgaccggtgc ctgcgtttgc ggtgtttcac gcacccattt ttgcaccagc
     6061 gtcgcaatca gcaacaccat caccagcccg gccaccagac gaatcgccgc aaaaccccag
     6121 ccgaggacaa accccataaa caccagcgtc gccgggttta acaccggatt gcccatccag
     6181 aatgccagcg caccgcccat cgatacctgt tggcgacgca ttcccgccgc gaccggagcc
     6241 gcacagcagg tacacatcat gcccggcagc gaaaacagcg ttcccagcag cgtgccgcga
     6301 aagcgcgatt gtccaagcgt acgcaacaac cagtcacgcg ggatcagcac ctgaatcaac
     6361 gaaccgagga tcacccccag caccgccgct ttccataccg cgaggaaata gatcatcgcg
     6421 taatccaacg ccgcctgcca cggattagca tccgcctgcg caaggataga tttaccgata
     6481 ctgtgggttt cggcagcagt aaacgctttg ccgtagtaag gttgccattt cacataccag
     6541 agaccggcaa tgacaacgag aaagaaaagc gcgggtttcc accactgaat gggcgttgcc
     6601 gcctgagatg aagactgacc agtcatagca ttccccagga ataagttatg ataattgagc
     6661 gcgtgaatat tacgctcact atcaattctt gggaataatt attcagctct tttgcgtaat
     6721 tctgaagagc ttaaaatagt cacgccttca tgctcaggcc gcatcgcctc tgccagcatg
     6781 acccgcgcca cgtctctcgc atcaatggat ttccagttac cgggcagtaa acgaaacagc
     6841 ggcgcaaaaa gcgtttcgtt catccgctgt ttgctacgat cgcccagcaa catcgacggg
     6901 cgagcaatgg tcaatttcgg ccagttctgg gcaattaatg cttcttccat ctcccctttg
     6961 acgcggttat agaaaaacgg cgagtgggca ttggcaccca tagcactgac caccagcata
     7021 tgctgcgcgc ccagtcgccg cccggttaat gcggtatcca ctaccagcgt gtaatcggca
     7081 tgaataaacg cctctttgct ccccgcttct cgccgcgtgg tgccgagaca acaaaacaca
     7141 atgtcgatgg gttcggtgac ctgcgccaac gcgtcactca gttgcggatc atggggatta
     7201 aacaccccgg gcatatcgcc caacggacgt cgcgtcggcg cggctatggc gttaactttc
     7261 ggttcgttaa tcaacatccg cagcaggtga ccgcccacca gccccgttgc gcctgtaatc
     7321 agtacctgac tcatcttcgc tcctttacag aattgtctgc cttgcgctcc acggctcatg
     7381 caccaggctt aatagaccat gaggtaatta tccccgattg tgtgaaattc gcctcatcca
     7441 atgcaacaac gcggaggaag catgagtaag aaaattgccg ttttaatcag tgatgaattt
     7501 gaggattcag aatttacttc acccgcagac gagttccgta aagccggaca cgaagtgatt
     7561 accattgaaa aacaagcggg taaaacggtg aaaggcaaaa aaggagaagc cagcgtgacc
     7621 atcgataaat ccatcgatga agtgacgcca gcggagtttg atgccctgct gctaccgggt
     7681 ggtcattcac cggattatct gcgtggcgac aaccgttttg tcacctttac ccgtgatttt
     7741 gtgaatagcg gcaaacctgt tttcgccatc tgtcacggtc cgcagctgct gatcagcgcc
     7801 gatgtgattc gggggcgcaa actgaccgca gttaaaccga tcattattga tgttaaaaat
     7861 gcgggcgcgg aattttacga tcaggaagtc gtggttgata aagatcagct ggttaccagc
     7921 cggacaccgg acgatctgcc agcgtttaac cgcgaggcgt tacgcctgct cggtgcctga
     7981 gtcgcgtagc caaatcattt ttttaccaaa gcccagcgtg ttgtcggtga atttgatttc
     8041 atcgagccgg atttcccaca ccggggccga cagcattctg gcaaccggaa agcgacgatt
     8101 gtacgcctgg cgcgcgaggt cgctttcctc accttccagc ctgcggatct cacctttaaa
     8161 ctgcacgccg cgaattaacg ctaccgtttt cggctgaccg tttaccgttc cggcaaccgc
     8221 agcctgcggc ccactcatct gcgcgtggcg cgttttttct tccgtcaaaa tgtagaaggc
     8281 gactttctgc gcatcaaaca gataaaaagc attagcgcac cacagttccc cttcctgctg
     8341 cacacaccag gtgacaacat gttgttttgc caaccaacgg ctgatggtga tgagtgtttc
     8401 cattgctgct ctcttttata ctgtgggcct gaccttaaca tgcgacgcag acgatgacac
     8461 cctggtttct ttacttgatc cgtaccgccg acaataagct ttataccggg atcaccacgg
     8521 atgtcgaacg ccgctatcag cagcaccaaa gcggcaaagg ggcgaaagca ctgcgcggga
     8581 aaggagaact gacgctggcg ttttctgcgc cagtgggcga tcgttcgctg gcgttacggg
     8641 cggaatatcg cgttaagcaa ctgaccaaac ggcaaaaaga gcgtctggta gcggaaggcg
     8701 cagggtttgc agagctgtta agcagtctgc aaaccccgga gattaaaagc gattgaaatg
     8761 ctcgtgatac tcaaccagac cggttacgcc attcagcgcg tcatccgcta aacgatgtac
     8821 ctggaaggcg ctttcagtgc ccggccagcg gcaacgcaga tcgtgatgcg ccgccagttc
     8881 aaagccgaaa cgactgtaca gcgccggatc gcccagcgtc accactgcgg catagccgaa
     8941 ctcattaagc gaatcgagtc cttcatagac cagttggcgt gccagcccct gtccacggta
     9001 tttttcatca accgccaacg gtgccatgcc gacccattgc aggtcttcgc cctgcacatc
     9061 aaccggacta aatgccacat agccaatgac ctgaccttcg tcatctgtcg ccaccagccc
     9121 cagcgtcaga aagccatctt cacgcagatc gtgaaccagc ttcgcttccg catcactttc
     9181 gaatgagcga cgcagcaggg catcaatacc cggcgcatca atgggaattt ctactcgaat
     9241 tagcatggtt cacctaccga tgtctgtttg gtttcaggcg cggttttcat tcccgcctca
     9301 acaaaatccg ccagttgcag cagcatcatg cgtaaggctt tcggcatttg ctccagttca
     9361 atggcgtcca tcaggttttt cacatacagc cccagctccg tatcgccttc aatcaccagc
     9421 cgacgctgga agaagagcgt atccggatct tgtttacgcg ccgcaatcat cagcagatcg
     9481 ctggcgtcgg cactaaaact cacatcagct tgcgcgttct ggctgaccac cagtttgcca
     9541 ttcaccaccg aggtaaacca ttgcaggtca atatcacgca catgaatact taaccagcgg
     9601 ccttcaagaa actccagctc accatcatcc agcgcctgac ggaattgcca gcttaagacc
     9661 tgctcaagaa cctggcgttt tagcgcaaat ggcgtcagtt ttaccggtac actcaacaga
     9721 gatggcccca aatgcacaat acgggaacgc agtttatcca acacgagttt tactccctgt
     9781 ttcaacaatc atcctatttt gccatatcag aaaaataaca tagcggtata aatcaacaat
     9841 tccatatgaa attgctgcta ccaccaatac aactttaact gccttaaatc aaaaattgtc
     9901 gcagcaaggt taactaaaat cccagttcgt taacattttt gcgttttgaa agcgcaacct
     9961 tcaggaaaaa ttatggagct gctctgccct gccggaaatc tcccggcgct taaggcggcc
    10021 atcgaaaacg gcgcagatgc tgtttatatc gggctaaaag atgatactaa tgcccgtcac
    10081 ttcgccggcc ttaactttac cgagaaaaaa ttgcaggaag cggtgagttt tgtccatcaa
    10141 catcgccgca aacttcacat cgcgattaac acttttgcgc atccggacg
//
LOCUS       DAJTZL010000145         9850 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.145, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000145 DAJTZL010000000
VERSION     DAJTZL010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9850
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.145"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(382..1359)
                     /gene="epmA"
                     /locus_tag="OO544_003199"
     CDS             complement(382..1359)
                     /gene="epmA"
                     /locus_tag="OO544_003199"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HCR6768726.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1684..3492
                     /gene="frdA"
                     /locus_tag="OO544_003200"
     CDS             1684..3492
                     /gene="frdA"
                     /locus_tag="OO544_003200"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HCR6768727.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQECFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            3485..4219
                     /gene="frdB"
                     /locus_tag="OO544_003201"
     CDS             3485..4219
                     /gene="frdB"
                     /locus_tag="OO544_003201"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HCR6768728.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALVYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4230..4625
                     /gene="frdC"
                     /locus_tag="OO544_003202"
     CDS             4230..4625
                     /gene="frdC"
                     /locus_tag="OO544_003202"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HCR6768729.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4636..4995
                     /gene="frdD"
                     /locus_tag="OO544_003203"
     CDS             4636..4995
                     /gene="frdD"
                     /locus_tag="OO544_003203"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HCR6768730.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            5058..6191
                     /locus_tag="OO544_003204"
     CDS             5058..6191
                     /locus_tag="OO544_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HCR6768731.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLTPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            6280..6813
                     /gene="blc"
                     /locus_tag="OO544_003205"
     CDS             6280..6813
                     /gene="blc"
                     /locus_tag="OO544_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HCR6768732.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(6810..7127)
                     /gene="sugE"
                     /locus_tag="OO544_003206"
     CDS             complement(6810..7127)
                     /gene="sugE"
                     /locus_tag="OO544_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HCR6768733.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7302..7448)
                     /gene="ecnB"
                     /locus_tag="OO544_003207"
     CDS             complement(7302..7448)
                     /gene="ecnB"
                     /locus_tag="OO544_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HCR6768734.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7559..7684)
                     /gene="ecnA"
                     /locus_tag="OO544_003208"
     CDS             complement(7559..7684)
                     /gene="ecnA"
                     /locus_tag="OO544_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HCR6768735.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(7736..8302)
                     /gene="efp"
                     /locus_tag="OO544_003209"
     CDS             complement(7736..8302)
                     /gene="efp"
                     /locus_tag="OO544_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HCR6768736.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8344..9372
                     /gene="epmB"
                     /locus_tag="OO544_003210"
     CDS             8344..9372
                     /gene="epmB"
                     /locus_tag="OO544_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HCR6768737.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPGELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPILRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            9762..>9850
                     /locus_tag="OO544_003211"
     CDS             9762..>9850
                     /locus_tag="OO544_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6768738.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPR"
BASE COUNT         2517 a         2503 c         2502 g         2328 t
ORIGIN      
        1 aggcgaatgg aataaagaat aacaatgcag aaaatatata aaagggaatc gcactataac
       61 ccattaagta ataagccgat gggctattgg caaatccaaa tacggaagta aagatcatca
      121 atatgagtcc tatcagactc atctttttta tcgtgtgagg catgaaacca tccttcattt
      181 cagcgcgtgt ataacacgtg cccactgaac tgcactgcgc aacattgagc aggcagcgta
      241 aaaaccaata cagaattgat acccggatga tatcaaatat ccagtcttaa attgtggcct
      301 ttaacggaaa aatgaaaaca tatgctacag aatggcgctt atcacgccat tcttcgctgt
      361 taattcagta atttttcaga attatgcccg gtcaacgcta aaggcgatga cttcagccag
      421 tgtctccgcg cccagcgcca acatcaccag acgatcaaca cctaatgcca cgccggaaca
      481 gtcaggcata ccgactttca aggcttcaat cagattctgg tcaatggggt gctgcggcaa
      541 accgcgcgcc gcgcgcttac ggttatcttg ttcaaagcgt tgttgctgct cacgggcatc
      601 cgttaattca tggaaaccat tcgccagctc aataccttta taataaacct caaagcgttc
      661 agcgacccga tgatcttcgg tactgatttg cgccagtgat gcctggctgg ctggaaagtg
      721 gtacacaaag gtcggttttt ctttgccaat atttggctct acgccaaagg taaacagcaa
      781 ttgtagcaac gtgtcgcggt cttcttcggt atcagcaacg ttactcaaat ccagtttcgc
      841 tgcgacttcc cgcagttgcg ttttgtcggc agagagcggg tcaatttcca gataacgcaa
      901 gaaagcttgt tgataagaaa ggctttctgc tgccgggcag tccagcacct gttgtaagag
      961 atcgtccacc tcgttcatca accggtacat atcatagtgc ggtcgatacc actcaagcat
     1021 agtgaactca gggttgtgat aacgccccat ctcttcatta cggaagctgc ggcacagctg
     1081 gaataccggc ccacaaccgg caaccagcag gcgtttcata tggtattccg ggctggtcat
     1141 taaccagaga ttcatcccct gcgaatgccc ggggccaacg aaacgtgtct caaacgggac
     1201 caaatgaata tcggttaccg tcgcctggct catacaaggc gtctccacct ccagcactcc
     1261 acgatcggca aagaaacgac ggatctccgc cataatcgcc gcacgtttta ataagttagg
     1321 aatggatgcg ctcggctgcc aggatgccgt ttcgctcata gttaaatctc cagtttttga
     1381 caagggcacg aagtctactc gcaacgcgac ggcgagacaa attttacgca ggaatcaaac
     1441 agcggtgggc agtgactaaa aaaagcacga tctgatggtt tagtaattaa attaatcatc
     1501 ttcagtgata atttagccct cttgcgcact aaaaaaatcg atctcgtcaa atttcagact
     1561 tatccatcag actatactgt tgtacctata aaggagcagt ggaatagcgt tcgcagaccg
     1621 taactttcag gcacttaccc tgaagtacgg ggctgtggga taaaaacaat ctggaggaat
     1681 gtcgtgcaaa cctttcaagc cgatcttgcc attgtaggcg ccggtggcgc gggattacgt
     1741 gctgcaattg ctgccgcgca ggcaaatcca aatgcaaaaa tcgcactaat ctcaaaagta
     1801 tacccgatgc gtagccatac cgttgctgca gaagggggct ccgccgctgt cgcgcaggat
     1861 catgacagct tcgaatatca ctttcacgat acagtagcgg gtggcgactg gttgtgtgag
     1921 caggatgtcg tggattattt cgtccaccac tgcccaaccg aaatgaccca actggaactg
     1981 tgggggtgcc catggagccg tcgcccggat ggtagcgtca acgtacgtcg cttcggcggc
     2041 atgaaaatcg agcgcacctg gttcgccgcc gataagaccg gcttccatat gctgcacacg
     2101 ctgttccaga cctctctgca attcccgcag atccagcgtt ttgacgaaca tttcgtgctg
     2161 gatattctgg ttgatgatgg tcatgttcgc ggcctggtag caatgaacat gatggaaggc
     2221 acgctggtgc agatccgtgc taacgcggtc gttatggcta ctggcggtgc gggtcgcgtt
     2281 tatcgttaca acaccaacgg cggcatcgtt accggtgacg gtatgggtat ggcgctaagc
     2341 cacggcgttc cgctgcgtga catggaattc gttcagtatc acccaaccgg tctgccaggt
     2401 tccggtatcc tgatgaccga aggctgccgt ggtgaaggcg gtattctggt caacaaaaat
     2461 ggctaccgtt atctgcaaga ttacggcatg ggcccggaaa ctccgctggg cgagccgaaa
     2521 aacaaatata tggaactggg tccacgcgac aaagtttctc aggccttctg gcacgaatgg
     2581 cgtaaaggca acaccatctc cacgccgcgt ggcgatgtgg tttatctcga cttgcgtcac
     2641 ctcggcgaga aaaaactgca tgaacgtctg ccgttcatct gcgaactggc gaaagcgtac
     2701 gttggcgtcg atccggttaa agaaccgatt ccggtacgtc cgaccgcaca ctacaccatg
     2761 ggcggtatcg aaaccgatca gaactgtgaa acccgcatta aaggtctgtt cgccgtgggt
     2821 gaatgttcct ctgttggtct gcacggtgca aaccgtctgg gctccaactc cctggcggaa
     2881 ctggtggtct tcggccgtct ggccggtgaa caagcgacag agcgtgcagc aactgccggt
     2941 aatggcaacg aagcggcaat tgaagcgcag gcagctggcg ttgaacaacg tctgaaagat
     3001 ctggttaacc aggatggcgg cgaaaactgg gcgaagatcc gcgacgaaat gggcctggca
     3061 atggaagaag gttgcggtat ctaccgtacg ccggaactga tgcagaaaac catcgacaag
     3121 ctggcagagc tgcaggaatg cttcaagcgc gtgcgcatca ccgacacctc cagcgtgttc
     3181 aacaccgacc tgctctacac cattgaactg ggccacggtc tgaacgttgc tgaatgtatg
     3241 gcgcactccg caatggcacg taaagagtcc cgcggcgcac accagcgtct ggacgaaggt
     3301 tgcaccgagc gtgacgacgt caacttcctc aaacacaccc tcgccttccg cgatgctgat
     3361 ggcacgactc gcctagagta cagcgacgtg aagattacta cgctgccgcc agctaaacgc
     3421 gtttacggtg gcgaagcgga tgcagccgat aaggcggaag cagccaataa gaaggagaag
     3481 gcgaatggct gagatgaaaa acctgaaaat tgaggtggtg cgctataacc cggaagtcga
     3541 taccgcaccg catagcgcat tctatgaagt gccttatgac gcaactacct cattactgga
     3601 tgcgctggtc tacatcaaag acaacctggc accggacctg agttaccgct ggtcctgccg
     3661 tatggcgatt tgtggttcct gcggcatgat ggttaacaac gtgccaaaac tggcatgtaa
     3721 aaccttcctg cgtgattaca ccgacggtat gaaggttgaa gcgttagcta acttcccgat
     3781 tgaacgcgat ctggtggtcg atatgaccca cttcatcgaa agtctggaag cgatcaaacc
     3841 gtacatcatc ggcaactccc gcaccgcgga tcagggtact aacatccaga ccccggcgca
     3901 gatggcaaag tatcaccagt tctccggttg catcaactgt ggtctgtgct acgccgcgtg
     3961 cccgcagttt ggcctgaacc cagagttcat cggtccggct gccattacgc tggcgcatcg
     4021 ttataacgaa gatagccgcg accacggtaa gaaggagcgt atggcgcagt tgaacagcca
     4081 gaacggcgta tggagctgta ctttcgtggg ctattgctcc gaagtctgcc cgaaacacgt
     4141 cgatccggct gcggccattc agcagggcaa agtagaaagt tcgaaagact ttcttatcgc
     4201 gaccctgaaa ccacgctaag gagtgcaaca tgacgactaa acgtaaaccg tatgtacggc
     4261 caatgacgtc cacctggtgg aaaaaattgc cgttttatcg cttttacatg ctgcgcgaag
     4321 gcacggcggt tccggctgtg tggttcagca ttgaactgat tttcgggctg tttgccctga
     4381 aaaatggccc ggaagcctgg gcgggattcg tcgacttttt acaaaacccg gttatcgtga
     4441 tcattaacct gatcactctg gcggcagctc tgctgcacac caaaacctgg tttgaactgg
     4501 caccgaaagc ggccaatatc attgtaaaag acgaaaaaat gggaccagag ccaattatca
     4561 aaagtctctg ggcggtaact gtggttgcca ccatcgtaat cctgtttgtt gccctgtact
     4621 ggtaaggagc ctgagatgat taatccaaat ccaaagcgtt ctgacgaacc ggtattctgg
     4681 ggcctcttcg gggccggtgg tatgtggagc gcaattattg cgccggtgat gatcctgctg
     4741 gtgggtattc tgctgccact ggggctgttt ccgggtgatg cgctgagcta cgagcgcgtt
     4801 ctggcgttcg cgcagagctt cattggtcgc gtattcctgt tcctgatgat cgttctgccg
     4861 ctgtggtgtg gtttacaccg tatgcaccac gcgatgcacg atctgaaaat tcacgtgcct
     4921 gcgggcaaat gggttttcta cggtctggct gctatcctga cagttgtcac gctgattggt
     4981 atcgttacaa tctaacgtat cgccaatgta aatccggccc gcctatggcg ggccgttttg
     5041 tatggaaacc agaccttatg ttcaaaacga cgctctgcgc cttattaatt accgcctctt
     5101 gctccacatt tgctgcccct caacaaatca acgatattgt gcatcgcaca attaccccgc
     5161 ttatagagca acaaaagatc cccggtatgg cggtggcggt aatttatcag ggtaaacctt
     5221 attactttac ctggggctat gcggacatcg ccaaaaagca gcccgtcaca cagcaaacgt
     5281 tgtttgagtt aggttcggtc agcaaaacat ttacgggcgt gcttggtggc gacgctattg
     5341 ctcgagggga aatcaagtta agcgatcccg caacaaaata ctggcctgaa cttactgcta
     5401 aacagtggaa tgggatcaca ctattacatc ttgcgaccta caccgctggc ggcctgccat
     5461 tgcaggtgcc ggatgaagtg aaatcctcaa gcgacttgct gcgcttctat caaaactggc
     5521 agcctgcatg ggctccagga acacaacgtc tgtatgccaa ctccagtatc ggtttgttcg
     5581 gcgcactggc tgtgaagccg tctggtttga gttttgagca ggcgatgcaa actcgtgtct
     5641 tccagccact caaactcaac catacgtgga ttaatgtacc tcccccagaa gaaaagaatt
     5701 acgcctgggg atatcgcgaa ggtaaggcag tgcatgtttc gccaggggcg ttagatgctg
     5761 aagcttatgg tgtgaagtcg accattgaag atatggcccg ctgggtgcaa agcaatttaa
     5821 ccccccttga tatcaatgag aaaacgcttc aacaagggat acaactggca caatctcgct
     5881 actggcaaac cggcgatatg tatcagggcc tgggctggga aatgctggac tggccggtca
     5941 atcctgacag catcattaac ggcagtgaca ataaaattgc actggcagca cgcccggtaa
     6001 aagcgattaa gcccccaact cctgcagtac gcgcatcatg ggtacataaa acgggggcga
     6061 ccggcggatt tggtagctat gtcgcgttta ttccagaaaa agagctgggt atcgtgatgc
     6121 tggctaacaa aaactatccc aatccagcga gagtcgccgc cgcctgtcag attctcaacg
     6181 ctctacagta aaatttcatc gggtccgaat tttcggacct tttctgcgct tttccttgct
     6241 gtcatctaca cttagaaaaa aaccagtaag gaaacattta tgcgcctgct ccctctcgtt
     6301 gccgcagcga cagctgcatt tctggtcgtt gcctgcagtt ctcctacgcc gccgcgtggc
     6361 gtgacagtag tgaataattt cgacgctaaa cgctatctcg gtacctggta tgagattgcc
     6421 cgttttgatc accgttttga acgtggactg gaaaaagtca ccgcaacata cagtctgcgt
     6481 gatgacggcg gcctgaatgt catcaataaa ggctataacc ctgacagaga aatgtggcag
     6541 cagagtgaag ggaaagcgta ctttaccggc gcaccaacac gcgctgcgct gaaagtgtca
     6601 ttctttggtc ctttctatgg cggttataac gtcattgcac ttgatcggga ataccgccat
     6661 gcgctggttt gcggcccgga ccgcgactac ctgtggatac tctcccgcac gccaaccatt
     6721 tctgacgaag tgaaacagga gatgctggca gtcgcgaccc gggaagggtt tgatgtcagt
     6781 aagtttattt gggtacagca gcccggtagt tagtgagtgc tgagtttcag accaataatc
     6841 cccaatacga ttaacgccag actcgccagg cgcatcgggt tagcggactc accgagcagc
     6901 acaatgccgg tgatggccgc gccgactgcg ccaatacccg tccacacggc ataagccgtc
     6961 cctaccggta acgatttcat cgcccaggca agtagcgcca tactgactat catcgccgtc
     7021 acagtaataa cacttggcgt caaacgacta aagccgtggg tatatttcag gccaacggcc
     7081 cataccactt ccagcagacc agcaataact aagataatcc aggacatatc aggctccgga
     7141 gaattggggc catccccggt gaaaagaggc gtttgcaggt cgtcctgcaa agctgatggt
     7201 tgaacggata ttttgccagg tggcagggga atttcaacta ttacctttgt gactatcgca
     7261 aaaaaacaaa cggcacgaca taactgtcgt accgttattg cttattgctg cgctttcgtt
     7321 gctgcaccag aaatcgcgtt accgccatca gaaatgtctt caccaacgcc acgcgtggtg
     7381 ttgcaggcag ttaatactgt tgaaagcacc agaacagaaa agatcgctgc aattgtcttc
     7441 ttcaccataa cgtcttcctt ttaggtaatg ttatttatgt ttgcctatag caaattaata
     7501 atagacaaca tcgccaaaaa tgatggaaat ctgatgattt ttcggaagag aagaaaaatt
     7561 agctggcagc gcgggagatg gagttgccga gatgtttgat gtcttcgccg aaaccgcgag
     7621 cggtgttaca gcccgtgagc agcgtgctgg caagcaaaac aagaacgata aggcgtttca
     7681 tcatctctga cctgaataag tgatggtgca gcctgcaggc cgcaccacaa ccgcattact
     7741 tcacgcgaga gacgtattca ccagagcggg tatccacttt gatgacttcg ccgatttgta
     7801 cgaacagcgg aactttaacc acagcgccag tagacagggt agccggtttg ccgccagtac
     7861 ctgcggtatc acctttcagg cccggatcgg tatcaacgat ttccagttca acgaagttcg
     7921 gcggagtaac ggagatcggc tgaccattcc acagagttac gatacactct gcctgatcca
     7981 gcagccattt agcgttgtcg ccaattgctt ttgcatcagc agacagctgc tcgaaagttt
     8041 cgttgttcat gaagtgccag aactcaccgt cgttgtacag gtaagtcagg ttcatatcga
     8101 caacatcagc gccttcagcg gaatcagtag atttgaaggt tttttctacg cgagtaccgg
     8161 tcagcagacg acgcagttta acgcgagcaa atgcctggcc tttacccggt tttacgaatt
     8221 cactcgcttc aaccgcgtaa ggttcgccgt ctaacatgat tttaagacca gcacgaaaat
     8281 cgttgctata gtacgttgcc ataaggccct ctgaaatttg ttaattggta gctaagccac
     8341 aaaatggcgc atattgtaac cctaaatacc ccatccagag aagattggtt aacgcaactt
     8401 gccgatgttg tgaccgatcc tggtgaactt ctgcgtcttt tgaatataga cgcggacgaa
     8461 aaactgttgg ccggacgcag cgccaaaaag ctgtttgccc tgcgtgtgcc ccgctcattt
     8521 atcgatcgca tggagaaagg caatccggac gatcctattt tgcgtcaggt acttacctcg
     8581 caagatgagt ttgtcgtcgc gtccggattc tccaccgacc cactggaaga acagcacagc
     8641 gtagtgcctg gtttgttgca taaataccac aaccgggcgc ttttgctggt caaaggcggc
     8701 tgcgcggtaa attgccgcta ttgcttccgt cgccacttcc cctatgccga aaatcagggc
     8761 aacaagcgta actggcagac tgcacttgag tatgttgctg cgcatccgga actggacgag
     8821 atgattttct ccggcggcga tccgctgatg gcgaaagatc acgagctgga ctggttgctc
     8881 acacaactgg aagccatccc gcatatcaaa cgtctgcgga ttcacagccg tctgccgatt
     8941 gtgatcccgg cacgtatcac cgaggcgctg gttgaacgct ttgcccgttc tacgctgcaa
     9001 atcttgctgg tgaatcacat caatcatgcc aatgaggtag atgaaacgtt ccgtcaggcg
     9061 atggctaaat tgcgccgtgt cggtgtcacc ctgcttaacc agagcgttct gttacgtggt
     9121 gtgaacgata acgcacaaac gttggcaaac ctgagtaatg cgttgttcga tgccggcgtg
     9181 atgccgtatt acctgcatgt gctagataaa gtacagggcg cggcgcattt tatggtgagt
     9241 gatgacgaag cacggcagat tatgcgtgag ttgctgacat tggtgtcggg atatctggtg
     9301 ccgaaactgg cgcgagaaat tggcggcgaa cccagcaaaa cgccgctgga tctccagcta
     9361 cgccagcagt aactgattaa acagggaagt taaacattag cctgatgcgc tacgcttatc
     9421 aggcctacat tgtgcattga aattgaatgg atttgcaatg aactgtaggc cggataaggc
     9481 gtttacgccg catccggcaa gagcaacaaa cgcaagtaca gcaacctgtc attaaactgg
     9541 gaaatttaat ctttgcccgt tgcataacct ttaattattc atatacattg ctgttgttac
     9601 tctttatata gccaacacca ataattaacc tttcttttct gtttatatag agtgagtacg
     9661 gctatttatg ttattttaac aactattact accaagccca tttatctggc ttataaatta
     9721 aaattaatta tttaattgag aaatggttag ggagaaccta catggccata agcatcaagg
     9781 gtgttaatac cggtgttatt cgcaagagta ataatttcat tgcactggcg ctgaaaatta
     9841 aagaacctcg
//
LOCUS       DAJTZL010000146         9819 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.146, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000146 DAJTZL010000000
VERSION     DAJTZL010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9819
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.146"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(37..1032)
                     /gene="gapA"
                     /locus_tag="OO544_003212"
     CDS             complement(37..1032)
                     /gene="gapA"
                     /locus_tag="OO544_003212"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCR6768739.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            1374..1787
                     /gene="msrB"
                     /locus_tag="OO544_003213"
     CDS             1374..1787
                     /gene="msrB"
                     /locus_tag="OO544_003213"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HCR6768740.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSQTKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            1829..2101
                     /locus_tag="OO544_003214"
     CDS             1829..2101
                     /locus_tag="OO544_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HCR6768741.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKANCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            complement(2262..2903)
                     /gene="pncA"
                     /locus_tag="OO544_003215"
     CDS             complement(2262..2903)
                     /gene="pncA"
                     /locus_tag="OO544_003215"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HCR6768742.1"
                     /translation="MPPRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLNQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTSLDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(2914..3930)
                     /gene="ansA"
                     /locus_tag="OO544_003216"
     CDS             complement(2914..3930)
                     /gene="ansA"
                     /locus_tag="OO544_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HCR6768743.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASARGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            complement(4097..5953)
                     /gene="sppA"
                     /locus_tag="OO544_003217"
     CDS             complement(4097..5953)
                     /gene="sppA"
                     /locus_tag="OO544_003217"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HCR6768744.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            6114..6665
                     /locus_tag="OO544_003218"
     CDS             6114..6665
                     /locus_tag="OO544_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HCR6768745.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            6782..7825
                     /gene="selD"
                     /locus_tag="OO544_003219"
     CDS             6782..7825
                     /gene="selD"
                     /locus_tag="OO544_003219"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HCR6768746.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDATEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKATAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            7830..9791
                     /gene="topB"
                     /locus_tag="OO544_003220"
     CDS             7830..9791
                     /gene="topB"
                     /locus_tag="OO544_003220"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCR6768747.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIIPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERALDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLLQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDVVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEVGSGAIA"
BASE COUNT         2434 a         2450 c         2618 g         2317 t
ORIGIN      
        1 gaagtcgctc tttttagatc acagtttcat ctcaacttat ttggagatgt gagcgatcag
       61 gtccagaact ttgttggagt aaccggtttc gttgtcgtac caggatacca gtttcacgaa
      121 gttgtcgttc agagcgatac cagctttagc atcgaacacg gaagtgcaaa cttcgccgtt
      181 gaaatcggta gatactacgt catcttcggt gtagcccaga acgcctttca tttcgccttc
      241 agcagcggct ttaacggcag ctttgatctg ctcgtaagtt gcagcttttt ccagacgaac
      301 ggtcaggtca actacggata cgttcggggt cggaacgcgg aacgccatac cagtcagttt
      361 gccattcagt tctggcagta ctttacctac agctttagca gcaccggtag aggacgggat
      421 gatgttctgg gaagcgccgc ggccgccgcg ccagtctttg tgagacgggc catcaacggt
      481 tttctgagta gcggtagtag cgtgaacggt ggtcatcaga ccttcgatga tgccgaagtt
      541 atcgttgata actttagcca gcggagccag gcagttggtg gtgcaggaag cgttggaaac
      601 gatgtcctgg ccagcatatt tgtcgaagtt agcgccttta acgaacatcg gagtgttgtc
      661 tttagacgga ccagtcataa ccactttctt cgcaccagcg gtgatgtgtt tacgagcagt
      721 ttcgtcagtc aggaacagac cagttgcttc agcgacaacg tcaacaccaa cttcgtccca
      781 tttcaggtta gccggatcac gttcagcggt aacacggatt tttttaccgt taacgatcag
      841 atgaccgtct ttcacttcaa cggtaccgtc gaaacggccg tgagtggagt catatttcag
      901 catgtatgcc atgtaatcag cgtctaacag gtcgttgatt gcaacgatct cgatgtcaga
      961 acgtttctga gcagcacgga aaacaatgcg accgatacgg ccaaaaccgt tgatacctac
     1021 tttgatagtc atatattcca ccagctattt gttagtgaat aaaaggttgc ctgtaaaatt
     1081 acaaaaacct tacgcagcgt caagcggaat cgtgtcaatc attgcgacaa atcaatcctg
     1141 tgcctaagca ttacgcgact gactcgcctc actcttcctt tgggcttgaa accacatggg
     1201 gtcggcgccc cgaattttaa agggcaatta cgataaaaat gtgattttca tcacgattta
     1261 ggtgcagcca tttcgtcgag atcaagttta ggacaaaatt ttgccagttg gtgttaatgt
     1321 tttgttagaa tcagtcacgt aatgtgagca cgattaaagt gagatgtgag caaatggcta
     1381 ataaaccttc ggcagaagaa ctgaaaaaaa atttgtccga gatgcagttt tacgtgacgc
     1441 agaatcatgg gacagagccg ccatttacgg gtcgtttact gcataacaag cgtgacggcg
     1501 tatatcactg tttgatctgc gatgccccgc tgtttcattc ccaaaccaag tatgattccg
     1561 gctgtggttg gcccagtttc tacgaaccgg tgagtgaaga atccattcgt tatatcaaag
     1621 acttgtcaca tggaatgcag cgcatagaaa ttcgttgcgg taactgtgat gcccatctgg
     1681 ggcatgtctt ccctgacggg ccgcagccaa cgggcgaacg ttattgtgtt aactctgctt
     1741 ctttacgctt cactgatggc gaaaacggcg aagaaatcaa cggttgaaaa aacgattcag
     1801 caacttattc cacaggagcg gaaaaattat gaatcttgat gacattatca acagcatgac
     1861 gcctgaggta taccagcgtt tgtcgaccgc cgttgaactg gggaaatggc ccgatggcgt
     1921 tgcgttgacc gaggaacaaa aagcgaactg cctgcaactg gtgatgctgt ggcaagctcg
     1981 ccataatacc gaagcacagc atatgacaat tgacacaaat ggtcagatgg tcatgaagag
     2041 caaacagcag ttaaaagaag attttggtat ctcagcaaag cctattgcta tgtttaagtg
     2101 aatgagttat ctagtattta agcctgttat taagggaggg caaatatact tgttaggacg
     2161 tcgtgtttta gctgcaagta ggttaaatgc aacactgaca agtattatct ggcctacaat
     2221 gtcattgagg aaatgtaggc cagagatgaa ttcaactaat attacccctg tgtctcttcc
     2281 cagtctgcca gcgtatatag cgttgcccca gctgctgaca tctccataaa cgcgtgcgca
     2341 ctgtcctggg gctggatatt cacgccacga caaccatcgg taatcacatt taccttataa
     2401 cctaactgta acgcgtccag cacggtaaac ttcacgcaat agtcagtagc caggcccata
     2461 acgatcaatt catcgatttc atgatcgcgt aaccagtcat cgagcgaggt tttctgccga
     2521 cggccgttat caaaaaaggc actgtaactg tcaactaaag gattttcgcc tttatggaac
     2581 accgctgcga tcgctttttg gttcagtaac ggatgtaatt gtgcgccttc actgttctgc
     2641 acacagtgat ctggccagaa ggtttgtggc aaaccgtcga gttggcctgg cgtataaggc
     2701 tctacaccgt gctgactggc aaaactgccg tgattcgccg ggtgccagtc ctgactggcg
     2761 ataaccgctt caccgcgcga ctggcaccag tcaatcaggc ggttagcgac atccaccgta
     2821 ctgtcacctt ccggcacggc gagcgcgcca ccagcacaga aatcattttg taaatcgacc
     2881 agtaacaggg cgcgaggggg cattacagtc tccttaatca tccggcgtca gttcgccgcg
     2941 caggttttgg ctcatggcct tgcgaatggt ttcagtatcc agttcctggc tcagcaggta
     3001 atgcagtttg gttagcgtgg cttctacagt catatctgca ccgccaataa cgccggcatg
     3061 ggcgagggcg ttaccggtgg cataaccacc catattcact ttaccggaca tacattgtgt
     3121 caggttgacc accacaatac cgcgagcgct ggcttcttgt aattcctgca ggaaggcttt
     3181 gttttgtggc gcattaccca cgccatagga gcgcagaatc aatgctttca ccggttggcg
     3241 cagaaaattg cgcacgacgt cagcagaaat ccctggataa atcgtcacta cgccaattgg
     3301 ttgtggcgtg attggatgaa cgattaattc cccttcaccg tgcggggcgg gtggcgtatt
     3361 caaacgacga atatggatac cagcttccag taacggagga aggtttggag aggcaaacgc
     3421 atcaaaacca tcggcatggg ctttggtagt gcggttgccg cgatacaatc ggttattgaa
     3481 aaagagcgtt acttcgttga tcggataatt cgccgcaacg tacaacgcat tcagcagatt
     3541 aatttgtccg tcagagcgta actcagccag cgggatttgt gaccctgtca caatgaccgg
     3601 tttaccgaga ttctcgagca tgaacgacag cgcagaggca gtatacgcca tcgtgtcggt
     3661 gccgtgcagg atgacaaaac catcatagtc gtcatagtgc gctttaatat cttcagcaat
     3721 atgctgccag tcttctggcg tcatatcaga agaatccatc agcggcgtat attcatgaat
     3781 ggtgaaatct ggcatctccg ggcgatggaa ttccggcatc agcgccagtt ggcgttgtag
     3841 atgacctgac accggaatat aaccctgctc ggaacgctgc atcccgatgg tcccgcccgt
     3901 gtaggcgacg taaattgatt tcttttgcat gatattgata tgaatgatat cgaaagagca
     3961 aaagtatata cgtgaggcag gaggagcgca cctgatacta tgcggataag gaaatatggg
     4021 gcgagacagc gggaagagga ttcgtcgacg taaaaaataa tgtggccgac gatctgtcgg
     4081 ccactcagta caagacttaa cgcacgttgg cgcaggtcag gcaaaacgca taacggtttt
     4141 gtgggtcgtt aaacgcggcc agcttgtcgc tttcactttt aacggtagag gctaccgagg
     4201 caagcggtgc aggtaacatg gcctggaacg catctggcaa cattgcccgg acagaaccag
     4261 acatgttgtc catcactttg tcgaagaagg tcggttcatc aacgtagtat tccagatgcc
     4321 actgtttcac ttttgccagc tctgctgctt tggcgaccgc atcatcgaaa tccccgagac
     4381 tatcgaccag cccgttagct tttgcatcct gaccggtcca gacgtggccc tgggcgattt
     4441 tatcaatctg ctccggcgtc gaatgacgcg catcagcaac cagcgtgata aagcgtttat
     4501 agccattctc aatgcttaat tgcatcatct gctgcgcttc cggcggcagt gccctggtga
     4561 tagaaacatc cgccagcggt gaagttgaga caccatcagt atgaacacca atcgaatcca
     4621 gactattttc tacggtggtg atcacgccga agataccgat agaaccggtc agggtgctgg
     4681 ggttagccac aatgtaatta gctggcgtgg aaatccagta accaccagat gccgccatgc
     4741 cgcccatcga tacaaccaca ggcttacccg ctgcccgggc tgctgccagt tcagcgcgaa
     4801 tcacttcaga cgcggtaacg ctgccgcctg ggctattaac acgcaggaca atcgctttca
     4861 ctttcgggtc aaggcgagcg tcgcggattt gtgccgcagt ggtatcaccg ccaacattcc
     4921 cctgagtttc ctcgccatcc ataattgcgc cattagcgaa gacgacaccg atgctgtcac
     4981 cggtatctgc cggcgttttc aatgcgtaat cgtaataact gatggcgcga taatttttat
     5041 cagtcttact ccagccgaac tctttggtta gagctttttc gatttcggca ctcgatgcca
     5101 gtgcatcgac cagcttgttt tccagtgcat atttcgcggt atcgccaccg gttttggtta
     5161 aaccctcaag caacccttgc gcgccaggga atacctgctg agcagggatc tgccggttag
     5221 cggcaacagt attcagatag ttttgccaca gctcaccaat ccagcggctg tcagcttcgc
     5281 gggctgccgg tgacatgtca tcacgaataa acggttcaac ggcagattta tacgtaccca
     5341 cgcggaacac atgggtggaa actttcagct tatccagcaa cgatttgtag tacagaccgt
     5401 tggtagcaaa accgtgcaaa tccaccacgc cttgcggtga cagccaaatt ttattggcga
     5461 aactggcgag ataatattgc ccctggctgt agttctcgcc aacggcataa accggtttcc
     5521 cgctgtcacg aaactctttc agggctttgc cgatgtactg catagacggc tggtcgccgc
     5581 ctgcgaagtt tttcagatcc atcacgatac cggtgatatt gcggtcgtcc ttcgcctggc
     5641 gaatggtgtt gacgatatca aacagtgagt tttcctgcag acgatcggaa ctggcaccaa
     5701 gcagctggcg gcttaattta ctaaaccgct gagaactgtc gggtttatcg acgatcacac
     5761 cagaaatgtc cagcagcagt gcgccacgac tggccgtttc tttcgaatca ccaccactga
     5821 cctgcatcca gatccccaca ccaaccagta cgaggaaaat aaagaacagg ttaagtacca
     5881 tttcacggac gaaattcagc agacgccacg tccatttaaa aaatccggca ataaatcgcc
     5941 aaagggttcg catgtattct cccaacttaa ggtcacacct gtcacaggcg caatagatgt
     6001 ggttatcgta atgacacaac cggcacttgt cagcaggaat tgcggactgt gctgtaacaa
     6061 aatccactct cgtgttaatt ttgtgaataa atatcacgac aggagttaat caaatggatg
     6121 cactcgaact attgatcaat cgccgtagcg cctcccgcct ggctgaaccc gcgccaacgg
     6181 gtgaacaact gcaaaacatc ctgcgtgcgg gtatgcgtgc gccggaccat aagtccatgc
     6241 aaccgtggca tttttttgtg attgaagggg aagggcgcga gcgtttcagc gccgtactgg
     6301 aacagggggc gattgctgcc ggtagtgatg acaaagctat cgacaaagcc cgtaatgcgc
     6361 cgttccgcgc accgctcatc atcacggtgg tggcgaaatg cgaagaaaat cataaagtcc
     6421 cgcgatggga acaggaaatg tctgccggat gcgcggtcat ggcgatgcaa atggcagccg
     6481 ttgcccaggg atttggcggc atctggcgca gtggcgcatt aactgaaagt ccggtagtgc
     6541 gtgaagcatt cggttgccgt gagcaggata aaattgtcgg ttttctctac ctcggcacgc
     6601 cgcagttgaa agcatctacg tcgattaacg tcccggaccc gacgccgttt gtaacttatt
     6661 tctgataatt gtcgcgaaac tgtctggatt gtgagcaaag gcgcaggaat tcagacactc
     6721 tcacttatca cttcacggaa tgagggttac catacccgga ttgcattgac aggagatgtc
     6781 catgagcgag aactcgattc gtttgaccca atacagccac ggagctggtt gcggctgtaa
     6841 aatttcccca aaagtgttgg aaaccatcct gcacagtgag caggcgaagt ttgttgatcc
     6901 gaatttgctt gtgggtaatg agacccgcga cgatgcggcg gtgtacgatc tgggcaatgg
     6961 caccagcgtt atcagtacca ccgacttctt tatgccgatc gttgataatc ctttcgattt
     7021 tggccgcatt gcggcgacta acgccatcag cgatatcttc gcgatggggg gcaaaccgat
     7081 tatggcgatt gcgatcctcg gctggccgat taacaaactt tccccggaaa ttgcccgcga
     7141 agtaaccgaa ggtggacgct atgcatgccg tcaggcgggt attgcgctgg ctggcggtca
     7201 ctccatcgat gcgacggagc cgatttttgg tctggcggta acggggatcg taccgaccga
     7261 gcgggtgaag aaaaacagta ccgcacaagc cggatgcaaa ctgttcctga cgaaaccgct
     7321 ggggatcggc gttcttacca cggctgagaa aaaatcactg ctgaaaccag aacatcaggg
     7381 actagcgacg gaagtgatgt gccggatgaa catcgcaggc gcgtcctttg ccaacatcga
     7441 aggcgtaaaa gcgatgaccg acgttacggg ttttggtctg ctgggccatt tgagcgaaat
     7501 gtgtcagggc gcaggtgtgc aggcgcgcgt tgactatgac gcgatcccaa aattgccggg
     7561 ggttgaagag tacattaagt tgggcgcagt gcctggcggc accgaacgta actttgccag
     7621 ctacggtcat ctgatgggtg aaatgccgcg tgaagtgcgc gatttgctgt gcgatccgca
     7681 aacttctggc ggtttgctgc tggcggtcat gccggaagca gaaaatgagg tcaaagctac
     7741 agccgccgag tttggcattg aactgacggc aattggcgaa ctggtgccag cgcgcggcgg
     7801 tcgtgccatg gttgagattc gttaattcaa tgcggttgtt tattgccgaa aaaccgagtc
     7861 tggcgcgcgc cattgctgat gtcctgccca aaccgcaccg gaaaggcgat ggctttatcg
     7921 agtgcggtaa tggtcaggtg gtgacctggt gtatcggtca cctgcttgag caggcgcagc
     7981 cagacgccta cgacagccgc tatgcgcgct ggaatcttgc ggatttgccg attatcccgg
     8041 aaaagtggca attacagccc cgaccctccg tgaccaaaca acttaacgtc atcaaacgct
     8101 tcctgcatga agccagcgaa atcgttcacg ccggggaccc ggatcgtgaa gggcaactgc
     8161 tggtggatga agtgctggac tatctgcaac tggcaccgga aaagcgccag caggtgcagc
     8221 gttgcttgat aaacgacctg aacccgcagg cggttgagcg ggcgctcgac cgtcttcggt
     8281 ccaacagtga gtttgtgcca ttgtgcgttt ctgcgctggc gcgagcgcgt gccgactggc
     8341 tgtacggcat caatatgacc cgtgcgtata ccattctcgg tcgcaatgcc ggttatcagg
     8401 gcgtactttc cgtgggacgc gtgcagacac ccgtgctcgg gctggtggtg cgccgcgatg
     8461 aagagattga aaacttcgtg gcgaaagact tctttgaagt caaagcacat atcgtgacac
     8521 ctgccgatga gcggtttacc gctatctggc aaccgagcga agcgtgtgaa ccgtaccagg
     8581 atgaagaagg gcgcttgtta catcgtccac tggcggagca tgtggttaac cgcattagtg
     8641 gtcaaccagc aattgtcacc agctataacg ataaacggga atcagaatcc gcgccgctgc
     8701 ctttttcgct ttcggcgttg cagattgaag cggcaaaacg ctttggtctg agcgcgcaga
     8761 acgtgcttga tatctgccag aagctgtacg aaacgcacaa gctaatcact tatccgcgtt
     8821 ccgattgtcg ctatttgcca gaagaacatt ttgccggacg ccacgcggtg atgaatgcca
     8881 tcagcgttca tgcaccagat ctgttgctgc agccagtggt agatccagat atacgcaacc
     8941 gctgttggga tgacaaaaag gtcgatgcac accacgccat catcccgacc gcacggagtt
     9001 cagccatcaa cctgacggag aacgaagcga aggtctataa cctgattgcc cgtcagtact
     9061 tgatgcagtt ctgcccggat gtggtgttcc gcaagtgtgt tatcgaactg gacattgcca
     9121 aaggcaaatt tgtcgctaaa gcgcgttttc ttgctgaagc aggctggcgc acgctgttag
     9181 gcagcaaaga gcgcgatgaa gaaaacgacg gtacgccatt gcctgtggtg gcgaaaggcg
     9241 atgagttgct gtgtgaaaaa ggtgaagtgg tagagcggca aacccagccg ccgcgccatt
     9301 ttaccgatgc aacactgctt tcggcgatga ccgggatcgc gcgctttgtg caggacaaag
     9361 atctgaaaaa gatccttcgt gcgaccgatg gtctggggac agaagcaacc cgtgccggga
     9421 ttattgaact gctgttcaag cgtggttttc tgaccaaaaa agggcgctat atccactcta
     9481 ccgacgccgg aaaagcgtta ttccattcgc tgccagaaat ggcgacgcga ccggacatga
     9541 ccgcgcactg ggaatcggtg ctgacgcaaa tcagcgaaaa gcagtgtcgc tatcaggact
     9601 ttatgcagcc gctggtgggg acgctatatc agcttattga tcaagccaaa cgtacgccgg
     9661 tgcggcagtt tcgcggcatt gtggctccgg gcagtggtgg cagtgctgat aagaaaaagg
     9721 ctgcaccgcg taaacgtagt gcgaaaaaaa gtccgccagc agatgaagtc ggaagcgggg
     9781 cgatagcgta agcgagtgaa tctttcgtgc tattcgagt
//
LOCUS       DAJTZL010000147         9817 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.147, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000147 DAJTZL010000000
VERSION     DAJTZL010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9817
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.147"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            235..1341
                     /gene="ompC"
                     /locus_tag="OO544_003221"
     CDS             235..1341
                     /gene="ompC"
                     /locus_tag="OO544_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HCR6768748.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNKSEDGDQTYVRLGFKGVTQVTDQLTGYGQWEYQIQGNTSEDNKENSWTRVAFAGL
                     KFQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFG
                     LVDGLNFAVQYQGKNGSVSGEGMTNNGRGALRQNGDGVGGSITYDYEGFGIGGAISSS
                     KRTDDQNSPLYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSLGWANKAQN
                     FEAVAQYQFDFGLRPSVAYLQSKGKNLGTIAGRNYDDEDILKYVDVGATYYFNKNMST
                     YVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            1453..2508
                     /gene="apbE"
                     /locus_tag="OO544_003222"
     CDS             1453..2508
                     /gene="apbE"
                     /locus_tag="OO544_003222"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HCR6768749.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGTKTDGAMDITVGPLVNLWGFGPEQQSVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLNVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGLPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLISEKN"
     gene            2582..3646
                     /gene="ada"
                     /locus_tag="OO544_003223"
     CDS             2582..3646
                     /gene="ada"
                     /locus_tag="OO544_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HCR6768750.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPRQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            3646..4296
                     /gene="alkB"
                     /locus_tag="OO544_003224"
     CDS             3646..4296
                     /gene="alkB"
                     /locus_tag="OO544_003224"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HCR6768751.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTGDCRYNLTFRQAGKKE"
     gene            4372..6015
                     /gene="yojI"
                     /locus_tag="OO544_003225"
     CDS             4372..6015
                     /gene="yojI"
                     /locus_tag="OO544_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HCR6768752.1"
                     /translation="MELLVLVWRQYRWPFISVMELSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAITLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAVT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFSYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            6233..7879
                     /gene="mqo"
                     /locus_tag="OO544_003226"
     CDS             6233..7879
                     /gene="mqo"
                     /locus_tag="OO544_003226"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HCR6768753.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDADKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLDLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
     gene            complement(8188..8676)
                     /gene="eco"
                     /locus_tag="OO544_003227"
     CDS             complement(8188..8676)
                     /gene="eco"
                     /locus_tag="OO544_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HCR6768754.1"
                     /translation="MKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAEKGMKRQV
                     IQLTPQEDESTLKVELLTGQTQEVDCNLHRLGGKLENKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAV
                     VR"
     gene            complement(8811..8975)
                     /locus_tag="OO544_003228"
     CDS             complement(8811..8975)
                     /locus_tag="OO544_003228"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6768755.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFAHISNGYKHIYIAINSPEI
                     FLLVCSLIRI"
     gene            9084..9578
                     /gene="napF"
                     /locus_tag="OO544_003229"
     CDS             9084..9578
                     /gene="napF"
                     /locus_tag="OO544_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HCR6768756.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECCFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            9568..>9817
                     /gene="napD"
                     /locus_tag="OO544_003230"
     CDS             9568..>9817
                     /gene="napD"
                     /locus_tag="OO544_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HCR6768757.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQG"
BASE COUNT         2341 a         2458 c         2641 g         2377 t
ORIGIN      
        1 tgatgttagg tgcttatttc gccattccgc aataatctta aaaagttccc ttgcatttac
       61 attttgaaac atctatagcg ataaatgaaa catcttaaaa gttttagtat catattcgtg
      121 ttggattatt ctgcattttt ggggagaatg gacttgccga ctgattaatg agggttaatc
      181 agtatgcagt ggcataaaaa agcaaataaa ggcatataac agagggttaa taacatgaaa
      241 gttaaagtac tgtccctcct ggtcccagct ctgctggtag caggcgcagc aaacgctgct
      301 gaagtttaca acaaagacgg caacaaatta gatctgtacg gtaaagtaga cggcctgcac
      361 tatttctctg acaacaagtc agaagacggc gaccagacct atgtacgtct tggtttcaaa
      421 ggcgtaactc aggttactga ccagctgacc ggttacggcc agtgggaata tcagatccag
      481 ggcaatacct ctgaagacaa caaagaaaac tcctggaccc gtgtggcatt cgcaggtctg
      541 aaattccagg atgtgggttc tttcgactac ggtcgtaact acggcgttgt ttacgacgta
      601 acttcttgga ccgacgtact gccagaattc ggtggcgaca cctacggttc tgacaacttc
      661 atgcagcagc gtggtaacgg cttcgcgacc taccgtaaca ctgacttctt cggtctggtt
      721 gacggcctga actttgctgt tcagtaccag ggtaaaaacg gtagcgtaag cggcgaaggc
      781 atgaccaaca acggtcgtgg tgctctgcgt cagaacggcg acggcgttgg cggatctatc
      841 acttatgatt acgaaggctt cggtatcggt ggtgcgatct ccagctccaa acgtactgat
      901 gatcagaaca gcccgctgta catcggtaac ggcgaccgtg ctgaaaccta caccggtggt
      961 ctgaaatacg acgctaacaa catctacctg gctgctcagt acacccagac ctataacgca
     1021 actcgcgtag gttccctggg ttgggcgaac aaagcacaga actttgaagc tgttgctcag
     1081 taccagttcg acttcggtct gcgtccgtct gtagcatacc tgcagtctaa aggtaaaaac
     1141 ctgggtacca tcgctggtcg taactacgac gacgaagata tcctgaaata tgttgatgtt
     1201 ggtgctacct actacttcaa caaaaacatg tccacctacg ttgactacaa aatcaacctg
     1261 ctggacgaca accagttcac tcgtgacgct ggcatcaaca ctgataacat cgtagctctg
     1321 ggtctggttt accagttcta atctcgattg atatcgaaca aagggcctgc gggccctttt
     1381 ttcattgttt tcagcgtaca aactcatttt tttggtgtac tcttgcgacc gttcgcatga
     1441 ggataatcac gtatggaaat aagctttacc cgcgtggcac tgctggctgc cgcgctcttc
     1501 tttgttggtt gcgatcaaaa accacaaccc gccaaaaccc acgctactga agttaccgtt
     1561 ctagaaggca aaaccatggg taccttctgg cgtgccagca tcccgggcat tgatgccaaa
     1621 cgcagtgccg aacttaaaga aaagattcag acccagctgg acgccgacga tcagctgctt
     1681 tcgacctata aaaaagattc cgcgctgatg cgctttaacg attcgcaaag tttatcgccg
     1741 tggccggtaa gtgaagcgat ggccgatatc gtcaccacct cgctgcgcat tggcacgaag
     1801 accgatggcg cgatggatat aaccgtcggg ccgctggtga atctgtgggg ctttggcccg
     1861 gaacaacagt cggttcaaat tccgagccag gaacagatcg atgcgatgaa agccaaaacc
     1921 ggcttacagc acctgacggt cattaatcag tcgcatcagc aatatctgca aaaagacctg
     1981 ccggatttaa atgtcgatct ctccaccgtc ggtgaaggtt atgcggcgga tcacctggca
     2041 cgcttgatgg agcaggaagg gatttcccgc tatctggtgt cggtgggcgg cgcgctgaac
     2101 agccgtggta tgaacggtga aggcctgccg tggcgggtag cgattcaaaa accaaccgat
     2161 aaagaaaacg cggttcaggc tgtggtggat atcaacggtc atgggattag cacctctggc
     2221 agctaccgta actattacga actggacggc aaacgtcttt cccatgttat cgatccgcaa
     2281 accgggcgtc ccatcgaaca caatctggta tccgtgacgg tgattgctcc gacggcgctg
     2341 gaagccgatg cctgggatac tggcttgatg gtactcgggc cggagaaagc caaagaggtt
     2401 gttcgccggg aagggctggc ggtttatatg atcaccaaag aaggcgatag ctttaaaacc
     2461 tggatgtcac cacagtttaa aagcttcctt atcagcgaaa aaaattaaag cgcaagattg
     2521 ttggtttttg cgtgatggtg accgggcaga ctaaaggcta tccttaacca gggagctgat
     2581 tatgaaaaac gccacatgct taactgacga tcaacgctgg caatctgtct tagcccgcga
     2641 cccgaatgcc gacggcgaat tcgttttcgc cgtgcgcacc acgggtatct tttgccgtcc
     2701 gtcttgccgc gccagacatg ccttgcggga aaatgtctcc ttctacgcaa atgccagcga
     2761 ggcactcgcc gccggatttc gcccctgcaa acgttgtcag ccagacaaag ccaatccccg
     2821 gcaacatcgg ttggataaaa tcacccacgc gtgtcgactg ctggaacagg aaacgcctgt
     2881 aacgctggaa gccttagccg accaggtggc gatgagtcca tttcatctac atcggttgtt
     2941 taaagcgact accggaatga cgcctaaagc ctggcaacag gcctggcgcg ctcgccgttt
     3001 gcgcgaatcg ctggcgaaag gggagagcgt gacgacgtct attcttaacg ccggattccc
     3061 cgacagcagc agttactacc gcaaagccga tgaaacgctg ggcatgacgg ctaaacaatt
     3121 ccgtcacggt ggcgaaaatc tggcggtgcg ttacgcgctg gctgattgtg agctgggtcg
     3181 ttgcctggtg gcagaaagcg agcgggggat ttgcgcgata ttgctgggcg atgatgacgc
     3241 gacattaatc agcgagttgc agcagatgtt tcccgctgcc gacaacgcgc ctgccgatct
     3301 gacgtttcag caacatgtgc gtgaagtgat cgccagcctc aatcaacgcg atacgccgct
     3361 gacgttaccg ctggacattc gcggcactgc ttttcagcaa caagtctggc aggcactgcg
     3421 cacgatacct tgtggtgaaa ccgtcagtta tcagcaactg gctaacgcca tcggcaaacc
     3481 gaaagcggta cgggccgttg ccagcgcctg tgccgccaac aagctggcta tcgtaatacc
     3541 ctgtcatcga gtggtccgtg gtgatggcac actttccggt taccgctggg gcgtgtcgcg
     3601 taaagcgcaa ctgctgcgcc gcgaagctga aaatgaggag aggtaatgtt ggatctgttt
     3661 gccgatgctg aaccgtggca agagccactg gcggctggtg cggtaatttt acggcgtttt
     3721 gcttttaacg ctgcggagca actgatccgc gatattaatg acgttgccag ccagtcgccg
     3781 tttcgccaga tggtcacccc cgggggatat accatgtcgg tggcgatgac caactgtggg
     3841 catctgggct ggacgaccca tcggcaaggt tatctctatt cgcccattga tccgcaaaca
     3901 aataaaccgt ggcccgccat gccacaaagc tttcatgatt tatgtcaacg tgcggctacg
     3961 gcggcgggct atccagattt ccagccagat gcttgtctta tcaaccgcta cgctcctggc
     4021 gcgaaactgt cgctgcatca ggataaagac gaaccggatc tgcgcgcgcc gattgtttct
     4081 gtttctcttg gcttacccgc gatttttcaa tttggtggcc tgaaacgtaa tgatccgctc
     4141 aaacgtttgt tgttggaaca tggcgatgtg gtggtatggg gcggtgaatc gcggctgttt
     4201 tatcacggca ttcaaccgct gaaagcgggg tttcatccac tcaccggcga ctgccgttac
     4261 aacctgacat tccgtcaggc aggtaaaaaa gaataaaaat aagaattatt attgctgtgc
     4321 gcgcgaagat gtttaaactg cgggctgtaa ttcattgtcc gggttttctg catggaactt
     4381 cttgtacttg tctggcggca gtatcgctgg ccatttatca gtgtgatgga gctaagcctc
     4441 gccagtgcgg ctttaggcat tggcttaatt gcttttatca atcagcgcct tatcgaaacg
     4501 gcggatacca gtctgctggt gttgccggag tttctgggat tattgctgct gttgatggca
     4561 atcactctcg gatcgcaact ggcgctcacc actttggggc atcacttcgt ttaccgactg
     4621 cgtagtgaat ttatcaagcg gattctcgat acccacgtcg agcgcattga acaactcggt
     4681 agcgcctcgt tgctggcggg attaaccagc gatgtgcgca atatcaccat tgcttttgtg
     4741 cgtctgccgg aactggtgca ggggatcatt ctcactatcg gttcagcggc gtatctgtgg
     4801 atgctgtcgg gcaaaatgtt gctggtaacg gctatctgga tggcggtcac catctggggc
     4861 ggttttgtgc tggtggcgcg ggtgtacaaa catatggcga ccctgcgtga aaccgaagat
     4921 aagctgtaca cggattttca aaccgtactc gaagggcgca aagagctgac tctgaaccgt
     4981 gaacgcgccg agtatgtgtt taacaacctc tacattcctg atgcgcaaga gtatcgccat
     5041 catattatcc gcgcagacac cttccatctt agtgccgtga actggtcaaa catcatgatg
     5101 ctgggcgcta tcggcctggt gttctggatg gcgaacagcc tcggttgggc tgataccaac
     5161 gttgctgcga cctattcgtt gacgctgtta ttcctgcgta cgccgctgct ttcggcggtt
     5221 ggcgcattgc cgacgctgct gacggcgcag gtggcgttta acaagctgaa caaattcgcg
     5281 ctcgcgcctt tcaaagcaga gtttccgcgc ccgcaggcgt ttcccaactg gcaaacgctg
     5341 gagctgcgta atgtgacgtt tagctatcag gataacgcgt tttccgttgg gccgattaac
     5401 ctcaccatta aacgtggcga gctgctgttt ctgattggtg gcaacggtag tggaaaatcg
     5461 acgctggcga tgttgctgac gggcttgtac cagccacaaa gcggcgaaat cttgctggat
     5521 ggcaaacctg tcagcggcga acaaccggaa gattatcgca aactgttttc ggcagtgttt
     5581 accgatgtct ggctgtttga tcaactgctg gggccggagg ggcaacccgc taacccgcaa
     5641 ctggttgaga agtggctggc gcagctgaaa atggctcata agcttgagtt aagcaacggg
     5701 cgtattgtta acctgaagtt atcaaaaggg cagaaaaaac gcgtggcgct gttgctggcg
     5761 ctggcagaag aacgcgatat tatcctgctg gatgaatggg cggcggatca ggatccacac
     5821 ttccgtcgtg agttttatca ggtgttgctg ccgctgatgc aggagatggg taaaactatt
     5881 ttcgccatca gtcatgatga tcattacttt atccacgccg accgcctgct ggaaatgcgc
     5941 aatgggcaac ttagcgagct gacgggcgaa gagcgcgatg ccgcttcgcg tgatgccgtt
     6001 gcccggacgg cataatatta cgacgctgac ctggcggact gctgccgcca ggtcaatatt
     6061 ctaaaaatcc cttcatttct aataacccta taattaatca acgaaattat aatgtttcta
     6121 aaattagaat ataatttata aacattattt aaatgttgtt actttagtgt taccgttgat
     6181 gccgcgcaaa tctcacctgc aataaagcga ctaaaagtaa ggcattaaca agatgaaaaa
     6241 agtgactgcc atgctcttct cgatggccgt ggggcttaat gccgtttcga tggcggcaaa
     6301 agcgaaagcg tccgaggagc aggaaactga tgtactgttg attggcggcg gcattatgag
     6361 cgccacgttg gggacctatt tacgcgagct ggagcctgaa tggtcgatga ccatggtgga
     6421 gcgcctggag ggtgtcgcgc aggagagttc gaacggctgg aacaacgccg gaaccgggca
     6481 ttctgcactg atggaactga actacacccc gcaaaacgcc gatggcagta tcagtattga
     6541 aaaagcagtc gccattaacg aagcatttca gatttcccgc cagttctggg cgcaccaggt
     6601 cgagcgcggc gtgctgcgta ctccgcgttc atttatcaat accgttccgc atatgagctt
     6661 tgtctggggc gaggataacg ttaatttcct gcgcgcccgt tacgccgcgt tgcaacaaag
     6721 ctcgctgttt cgcggtatgc gttactctga agatcacgcg cagatcaaag agtgggcacc
     6781 gttagtgatg gaagggcgcg atccgcaaca gaaagtggca gccacgcgta cggaaattgg
     6841 taccgatgtg aactacggcg agatcacccg ccagttaatt gcttccttgc agaagaaatc
     6901 taacttctcg ctgcaactca gcagcgaagt ccgcgcccta aagcgtaatg acgataacac
     6961 ctggaccgtt accgttgccg atctgaaaaa tggcactgca cagaacattc gtgccaaatt
     7021 tgtctttatc ggcgcgggcg gtgcggcgct gaagctgtta caggaatcgg ggattccgga
     7081 agcgaaagac tacgccggtt tcccggtggg cggacagttc cttgtttcgg aaaacccgga
     7141 cgtggttaat caccatctgg cgaaggttta cggtaaagca tccgttggcg caccaccgat
     7201 gtcggttccg catatcgata cccgcgttct ggacggtaaa cgcgtagtgc tgtttgggcc
     7261 atttgccacc ttctcaacca aattcctcaa aaacggttca ttgtgggatc tgatgagttc
     7321 caccaccacc tctaacgtga tgccgatgat gcacgtcggg ctggataatt tcgatctggt
     7381 gaaatacttg gtgagtcagg tgatgttgag tgaagaggat cgttttgaag cgttgaaaga
     7441 gtactatccg caggcgaaaa aagaggactg gcgtttgtgg caagcggggc agcgtgtgca
     7501 gattatcaag cgtgatgccg ataaaggtgg tgtactgcgt ctgggtactg aagtggtcag
     7561 tgaccagcaa gggaccattg ccgcgctgct gggggcatcg ccaggggcgt caaccgctgc
     7621 gccgattatg ttggatctgc tggaaaaagt atttggcgat cgcgtttcca gcccgcaatg
     7681 gcaggctacg ttgaaagcga tcgttccatc ttatggacgc aagctgaacg gtgatgtagc
     7741 ggcaacagaa cgcgagttgc agtacaccag cgaagtgctg ggcctgaaat acgacaagcc
     7801 gcaggcagca gatagtacgc cgaaaccgca gttgaaaccg caacccgttc aaaaagaagt
     7861 ggcggatatt gcgttgtaat gatgtgccac atccggcatg gtatgccgga tgtggcgtaa
     7921 ccgccttatc cgacctacgg ggtggagcac ttcacagcgg tgtaggcctg ataagacgcg
     7981 tcagcgtcgc atcaggcaac cggctcgggc gttagatgtc ggatgcggcg taaccgcctt
     8041 atccaaccta cggggtggag cacttcacag cggtgtaggc ctgataagac gcgccagcgt
     8101 cgcatcaggc aaccggcttg ggcgttagat gtcggatgcg gcgtgaccgc cttatccgac
     8161 ctacggggtg acgcacttca cagcagttta gcgaactacc gcgttgtcaa ttttctcttc
     8221 cgccttccag acgcggtact tcacatctac attgtctggc gtatacacca cgatcggcag
     8281 cttgctgttg taacgcagca ttccagcatc gcccagatac gcggtgacaa atttcttctc
     8341 tttcttgcca tccgggcagg ccatcatcgt ggaaaccggc gagctaactt tatcaaagac
     8401 ataataatca tagccccagc cttccagcgt tttgttttcc agcttcccgc cgagacgatg
     8461 caaattgcaa tcgacttcct gcgtctgacc ggttaacagt tctactttca gggtagattc
     8521 atcttcttgc ggggttaact gaatcacctg acgcttcatc cctttttcag cttgtggata
     8581 aggcgcgatt ttttccagtg gctggacgct ttctgccgcc caggcggaag tggtggcgaa
     8641 agcggcaaac aatactgcag gtagaatggt cttcattttt ttgctttcct tcttcattta
     8701 taggttttca ccgatgatgt cgctgaaggt taacatattc atcagattta ttaatttgct
     8761 ttatgaataa cttaaagaac attcagacaa ttttacctgc tcttttagtg ttaaattctg
     8821 atgagggagc aaaccagcag aaatatctct ggtgaattaa tcgctatata aatatgttta
     8881 taaccatttg aaatgtgagc aaaagcccgt ttttgccacg cttcccgcca ctcttttgat
     8941 cctgctacag gttttacccc gatcggggta tgcatctttg acacatcctt taatatctta
     9001 gcggctataa aaatggctta ttaattatgc ggtttatttg gtcgctctca attttcagag
     9061 cgcgttaatg atggaaggtc aatgtgaaga ttgatgcatc ccgtcggggc atactcactg
     9121 gtcgctggcg caaagccagt aacggtatcc gtccgccctg gtccggtgat gaatctcatt
     9181 ttctgaccca ttgcacccgt tgcgacgcct gtattaatgc ttgtgaaaac aacattctgc
     9241 aacgcggcgc gggcggctat ccgagcgtta atttcaaaaa taacgagtgc tgcttctgtt
     9301 acgcctgcgc gcaggcttgc cccgaatcgc tattttctcc gcgccacacc agggcctggg
     9361 atttgcagtt caccattggg gacgcgtgcc tggcgtatca gtcagttgaa tgccgccgct
     9421 gtcaggatag ctgtgagcca atggcaatta tctttcgccc gacgctctcc gggatctacc
     9481 agccgcaact taatagccaa ctttgcaacg gatgtggagc atgtgcagcc agctgtccgg
     9541 tatcagccat aactgcggag tatctccatg cacactaact ggcaagtttg cagcctggtc
     9601 gtgcaggcca aaagcgaacg aatttcagac atcagcaccc aactgaacgc ctttcccggc
     9661 tgtgaagttg ctgtcagcga cgcgccgagc ggtcagttga ttgtggtggt ggaagcagaa
     9721 gacagcgaaa cgctgatcca aaccattgag tcagtacgca acgtagaggg cgtgctggcg
     9781 gtgtcgctgg tttatcacca gcaggaagag caaggtg
//
LOCUS       DAJTZL010000148         9730 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.148, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000148 DAJTZL010000000
VERSION     DAJTZL010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9730
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.148"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(549..1100)
                     /locus_tag="OO544_003231"
     CDS             complement(549..1100)
                     /locus_tag="OO544_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR6768758.1"
                     /translation="MAILGYCRVSTDDQSITNQQMQIEEAGYNIAKWFADEAVSGSVK
                     ASLRNGFSSLLAYAREGDTVVVVAVDRLGRDTIDVLSTVKALQAKGVTVISLREGFDL
                     SSAMGEAMLGIMSTLAQLERSLIAERRKAGIERAKAEGVHMGRPVKASSEAVQMLISQ
                     GKTRLQIQEELGISRATYYRLAK"
     gene            complement(1929..2666)
                     /locus_tag="OO544_003232"
     CDS             complement(1929..2666)
                     /locus_tag="OO544_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein mom"
                     /protein_id="HCR6768759.1"
                     /translation="MGNRKQVTSRIISTPELIHYNDNIVGYGSRELRVETISCWLARL
                     VVVNKHYSHRFVNNSYLHLGIFSERELVGVMQWGYALNPNNGARVVTGTQNREYMELN
                     RLWLHDCMPRNSESRAISYALKLIRQLYPQVQWVQSFADERCGCLGVVYQASNFDYVG
                     SHETTFYELDGEWYHEICKNAIKRGGQRGEHLRTNIDRASVHKFRQFRYIRFLDKRAR
                     KRLNTKLFKVQPYPKPQTVKTGLKESE"
     gene            complement(2620..2820)
                     /locus_tag="OO544_003233"
     CDS             complement(2620..2820)
                     /locus_tag="OO544_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Com family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR6768760.1"
                     /translation="MYRNIRCRHCNKLLARASFSYLEVKCPRCKTLNQITSQSATEHP
                     TYTRKSCRGEQKASYIPYHQHA"
     gene            3110..3370
                     /locus_tag="OO544_003234"
     CDS             3110..3370
                     /locus_tag="OO544_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6768761.1"
                     /translation="MIENAEIYNDYFSDYSDACKYSSSAGEFICFNFFIKSYVFPPQQ
                     EDGGVPPPEITLHRIASVSMMEQQANELYSILGTILQKRESD"
     gene            complement(3538..3792)
                     /locus_tag="OO544_003235"
     CDS             complement(3538..3792)
                     /locus_tag="OO544_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HCR6768762.1"
                     /translation="MPEIKGTVTEDLVKQALYSGEVNKLLKAQVRKDFEAQIDTYVDE
                     VLAKLIGPAVAASDADNEPKTDAQPEQPDPAQPGTDSTMM"
     gene            complement(3828..4010)
                     /locus_tag="OO544_003236"
     CDS             complement(3828..4010)
                     /locus_tag="OO544_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012908619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HCR6768763.1"
                     /translation="MTFLQLILLYFCTAVCALYLVSGGYKAIRNYVRRKIEDAAAEKL
                     SKTAQVTPLPKDPTQP"
     gene            complement(4155..6209)
                     /locus_tag="OO544_003237"
     CDS             complement(4155..6209)
                     /locus_tag="OO544_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HCR6768764.1"
                     /translation="MSDSKWKMLSAMPGEFSVKVAGGTVAFIESPFRPSGNKGGITFA
                     DCVIRFSTKEPLWVMPVSGNPSAEITSSGVTGIIPITSDVAGTLTPSDWNAPDNAGPS
                     GSTNGSSSEPEYYFVVILAGQSNGMAYGEGLPLPDSFDRPDPRIKQLARRSTVTPNGT
                     SCTYNDIIPADHCLHDVQDMSGINHPKADLAKGQYGTVGQGLHIAKKLLPYIPQNAGI
                     LLVPCCRGGSAFTTGADGSFSEASGASADSSRWGAGKPLYQDLVSRTKVALAKNPKNK
                     LLAVVWMQGEADLASGSQQHNSLFTAMVQQFRTDLAPLAAQCVSGNATTVPWICGDTT
                     YYWKNAGTDKYEAVYGGYKGKEAQKIFFVPFLTDENGQSTPTNAPAEDPDIVAVGYYG
                     AASRTQGSFVSTQRDSHFSSWARRGVISDRLASAIMLHAGRTAELMRGQTVTPPDEKP
                     SPDTPSKPSTPPADTTTMSTLFAYRASESEGRLTPQGWAAGGGKAQIVDDAGASGGKA
                     MKLTKETGRAPWYLEHDAGNGADLLGKGGLVSCRFKLDGALTANQYALALYWPVSALP
                     QGVTLEGNAGHNLLASFYVQSDATDLNVMYHKGNTAQNTKLGSFGAFNNEWHTLGFRF
                     AGNNSIEVTPVIDGKDGTPFMLSQSPVGTFTADKLRVTDITKNATYPVLIDSIVVEVN
                     NA"
     gene            complement(6295..6867)
                     /locus_tag="OO544_003238"
     CDS             complement(6295..6867)
                     /locus_tag="OO544_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR6768765.1"
                     /translation="MLIGYIRVSTNDQNTALQRNALECAGCELFFEDRISGKTRERPG
                     LKRALKRLRPGDTLLVWKLDRLGRSMQHLVLLTEELRQRGVNFRSITDNIDTSTPMGR
                     FFFHIMGALAEMERELIIERTRAGLAAARAQGCHGGRPQKLTEKEREQIARLLANGES
                     RKQIALIFDIGLSTLYRYFPASPAPSPKVP"
     gene            6983..7306
                     /locus_tag="OO544_003239"
     CDS             6983..7306
                     /locus_tag="OO544_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6768766.1"
                     /translation="MPSDGTAVAANRLATPRNINGVPFDGTQDINITSGITQQDADSR
                     YIQDIRLGAENRQVMYGGAYYSAYGNVMAGLQIDSKVDASNDFIAFRPLQKLVGGTWI
                     TVSQL"
     gene            7358..7879
                     /locus_tag="OO544_003240"
     CDS             7358..7879
                     /locus_tag="OO544_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR6768767.1"
                     /translation="MELKNITRYYPENMPYGNNVQYFQSEDGKDFYESLPLFTKKYKL
                     CITPDSGVICSISQDASALYPAGFSVVEVDELPEGTDISGNWKFDNGIISRIPVNYAR
                     KLEAMRQSYLNQAYEKINDWRTELQLGTISDEDRAALTQWMAYISQVKKMELPAIKTE
                     AEFNAIKWPEQPQ"
     gene            complement(7911..8792)
                     /locus_tag="OO544_003241"
     CDS             complement(7911..8792)
                     /locus_tag="OO544_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6768768.1"
                     /translation="MFHVDNNSGVANMPALAPAQSNTTTWFTEGDGQKGISWIGQDWL
                     NILQAELLNILAEASIQPDKAQLNQLTLSIKAIIAANAFSRKNNLKEIADAGAEAQRL
                     ARGYLGLGTLATKNSLGPADVNALAKDQNLADLENKGTARNNLDVYSKSEGDNRYLRR
                     EQNGADIPDKGAFIDNVGLRETVNRAANALPSDGTAVAANRLANAHTINGVPFDGTQD
                     ITISSGTVTAIRLGSVTAHMPGTWESWDLNLGGGNVLTGIKVQDVGKNTADNVGGVYY
                     RPLQYLLNGAWVTAASI"
     gene            complement(8792..9352)
                     /locus_tag="OO544_003242"
     CDS             complement(8792..9352)
                     /locus_tag="OO544_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HCR6768769.1"
                     /translation="MAVTLTPHQRALLQLLPDGLAWDKRPSSVLASLCLGLSHSTARV
                     SWTGKQLLAERFPDTSRLLLEDWERYLGLPECDMAGATLTERQRYAGNKYRMKPSLNR
                     EFYIRFAAEFGYQIDIQPSPDSQWVSIVTINSETGYRNMNVLDDILTPLRIYEGGALE
                     CILNRYKPAWQTFIYVYANSHEEETI"
     gene            complement(9343..>9730)
                     /locus_tag="OO544_003243"
     CDS             complement(9343..>9730)
                     /locus_tag="OO544_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313014.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HCR6768770.1"
                     /translation="DNNPDIFPGDGDVQRVADYIRSHDDPATGQPVGQPLGPTVTVFK
                     LTNKPVPFSIRIIPKTPENQAAVKQALTDLLYNESRPGGLVLPSSFWRAVAGVKNLED
                     FEVRSPLTSVQAGDSELLTVGEITWL"
BASE COUNT         2469 a         2419 c         2201 g         2641 t
ORIGIN      
        1 aacccatgat tgacggctta tatcatcggt gaatcgtggt aacagcgtgt catgctcagt
       61 tgcagcacca tctagactga aaataaattc acggtgtaag ttcttaaata acaaaaatgg
      121 agttctccag taatcagaac ccccatgatg cttcccgatt aaaatatgct caattgacat
      181 aagaaataaa aatttttatt gcaatcactc ttgacaaggt ttactctctt ttaagagatg
      241 cttaacaggt tttctaaacg gatatccttt ctgggatatc tatcttttat cttaaaagtt
      301 tttacagata ctttaaagat atctgtatat attctgtaca gtaacttttc agagtctgaa
      361 aatttttata aaaaatgact agccctcttt gaatctcatc ttccccgaat cttcgaaaat
      421 atctgtctag ttgattcggt agaatctacc tcatgatgta tacgtgttta gtacccgtca
      481 caccagctat gtgaccccct tggcaaaaaa gccaaatatc agctacatcc atgtaactat
      541 tcagtaactt atttagcaag tctgtaataa gttgctctag agattcctag ctcttcctga
      601 atctgcaacc tcgttttacc ttgtgaaata agcatctgta cagcctctga agaagctttt
      661 acaggtcttc ccatatgtac cccttcagct ttagcacgtt caataccagc ctttctacgc
      721 tctgctatca agcttctttc aagctgtgcc aacgttgaca taatgcctaa cattgcttca
      781 cccatagcgc ttgataagtc aaatccttct ctaaggctga taactgtcac acctttagcc
      841 tgtagcgctt taaccgttga taacacatca attgtgtcac gtccaagacg gtctacagct
      901 accacaacta cggtgtcacc ttctcgtgca taagctaaca aactgctgaa accgtttctt
      961 aaagaagctt tgacagaacc actgacagct tcatcagcga accatttagc aatgttgtac
     1021 cctgcctctt caatttgcat ttgctggtta gtgatggatt ggtcatctgt actcaccctg
     1081 caatatccaa gtatcgccat aatgttcccc ttagaagtct caaaagttag tctcataagc
     1141 ttagctgtct cataatccaa aaacaacctt ttgagactac ttgagggtgc attctaccgt
     1201 tacgttgaaa gtgtctcaaa aggacttcct tatgagactg gttaatagag cgctatgaag
     1261 attcaggagg gtaactataa ccatcacttt ctaactttca cgtaggaaat cgttaagttt
     1321 cttgatagtt acaccatgtg accaactatc tatatatcgt tatatagccc tgtatatagc
     1381 actatatgta gtgtctggag agggacgact aacacgatac agtttaccct gaaaagagac
     1441 ttcacagatt ctttatatat ttcaataagt tatgctaaaa agagactata ccgatactgc
     1501 atagaatttg tgtaggattg catagatact tggaaaaaca ccgttaaatt gcatagtcat
     1561 tcacctgata ttgtcctgtt tttacaggta gttgattcgg atgttctagg aatcatcctc
     1621 atagttgatt cggttagttg ggcttgtacc aacccctaat caacctcata gtgtatttag
     1681 accttgatga ttttagacag ccgccctgtt catgcagcac gtccacacag tgaagctatt
     1741 cgcgacgctc aacgaaaaaa acctaaagtt ccggtacatg ctgtactgac ggcgacaaat
     1801 tatagcgcgg cgattttttt gataccgatt tgcatttcat ttgataccgc tgaaaaacgc
     1861 cgatacgcaa cttatttgat actaaatcgg cgcttttctt ttgagtttta gaatgagttt
     1921 ttaagcgttc attcactctc tttcaaacca gttttaactg tttgtggttt tgggtaaggc
     1981 tggactttga atagctttgt gttaaggcgt tttctggctc tcttgtccag aaaccggatg
     2041 taacgaaact gacggaattt atgaacactg gctctgtcga tattagtccg cagatgttca
     2101 cctcgctgtc ctcctcgttt gattgcattc ttgcaaatct cgtgatacca ctcgccatca
     2161 agttcataga atgttgtttc atgactgcct acataatcaa aattgcttgc ctggtacacg
     2221 acaccaagac agccacaacg ctcatctgca aacgactgaa cccactgcac ctgcggataa
     2281 agttgtctga ttagtttgag cgcgtaactg attgcccttg attcggagtt tctcggcata
     2341 cagtcatgca gccataaccg gttaagctcc atatattcgc ggttctgcgt gccagttacg
     2401 acgcgtgcac cattgtttgg attaagggca taaccccatt gcattacgcc aaccagctcc
     2461 cgttctgaaa atatgcccag atgaaggtag gaattattta cgaaacgatg gctgtaatgt
     2521 ttattgacga cgaccagccg ggccagccag caacttattg tctcaacccg taactcacgg
     2581 gagccataac cgacgatatt gtcgttatag tgaataagtt caggcgtgct gatgatacgg
     2641 gatgtaactt gctttctgtt ccccacgaca ggatttcctt gtgtatgtgg ggtgctctgt
     2701 ggcgctctga gatgtgattt gattgagggt tttacagcgc ggacatttaa cttccagata
     2761 actgaagctg gctcgtgcca gtagcttgtt acaatgtcgg caccgtatgt ttcgatacat
     2821 ggccgcataa cctcctgctt tgttgttgat atctttatca ctcaatcgat cgacaaaaac
     2881 gatcgatttg atttatttaa ttgatacaac aaataacacg aaatgtcatt agcgaaaatt
     2941 tatgagtttg atcactaatt ttaaacggtg ctgtaacgca taagtgctga atgattgtac
     3001 atttgaatac cgcaaagcaa acaaatcaac taatttatag aagtcaattg gttaattgcc
     3061 agcgtaagag gctgctatgg acaatcatgt aacgacgaga aatcgaaaaa tgattgaaaa
     3121 tgcggaaatc tataatgatt atttttccga ttattcagat gcctgcaaat acagttcgag
     3181 tgcgggagag ttcatatgct tcaacttttt tattaaatct tatgtttttc ctccacagca
     3241 agaagatgga ggtgtgccac ctcctgaaat cactttgcac cgtatagcct ccgtctcaat
     3301 gatggagcaa caggctaatg agctgtatag cattctggga acgattctac aaaagcgtga
     3361 atcggactaa tgataccggt tcatttgcct gcccggatgg ggaagcagga ttgactgctc
     3421 cataagcagc tatatttctg aatacgaaat actatccagc gattacatat ggctggatag
     3481 cggcaaagat gttttcatta gtgtcaaacc ggcagaggac accaccggtt tgtattgtta
     3541 catcatggta ctgtcagttc cgggctgagc cgggtctggt tgctctggct gcgcgtcggt
     3601 tttcggctca ttatcagcat cactggccgc aactgctggc ccaataagtt ttgccagcac
     3661 ttcatcaacg taagtgtcga tttgtgcctc aaaatccttg cggacctgcg ctttcagcaa
     3721 cttatttact tcaccggaat acagtgcctg ttttaccagg tcttcagtga cggtgccttt
     3781 aatttctggc atgtatatcc tccttatgcg ggaataaagc cctgcaatca gggctgggtc
     3841 gggtcttttg ggagcggagt tacctgcgct gttttactga gtttttcagc tgctgcgtcc
     3901 tcaattttgc ggcgaacata atttctgatg gccttataac caccactcac cagatataac
     3961 gcacatactg ccgtgcagaa atacaataaa ataagctgta aaaatgtcat tatccctccc
     4021 aattattgac atggtgttga caccggtaat atctgttagg taaaaaggtg cactgcattt
     4081 tttgctttgg atataacgat ctttttgccg ccggtttctt gttttccctt accggcggca
     4141 tttttttatc ctgcttacgc gttattcact tccaccacga tactgtcaat cagtaccggg
     4201 taagtcgcat ttttagtgat atcggtcacg cgcaacttgt ctgccgtaaa cgtgccgacc
     4261 ggagactgcg acagcatgaa tggtgtcccg tccttaccat caatgaccgg cgtcacctca
     4321 atactgttgt taccggcaaa acggaagccc agcgtatgcc attcgttatt aaatgcgccg
     4381 aatgacccca gcttcgtgtt ctgcgccgtg tttcctttgt gatacatcac gttaaggtct
     4441 gtggcatcgc tctgtacgta gaacgatgcc agcaggttat gaccggcatt tccttccaga
     4501 gtaacccctt gcggcagggc agaaaccggc cagtacagcg ccagtgcgta ctgattagcc
     4561 gtaagcgcgc catcgagttt aaaacgacaa ctgacaagac cacctttacc cagcaggtcc
     4621 gcaccattac cagcatcatg ctcaaggtac cagggggctc ttcctgtttc cttggtcagt
     4681 ttcattgcct tacccccgct ggctccggca tcatccacga tttgagcctt gccacctccg
     4741 gcagcccaac cctgtggtgt caaccgtcct tcagactcag atgcccggta agcaaatagc
     4801 gtactcatcg tcgtggtgtc tgcgggtggc gtggacggtt ttgatggtgt atcaggtgac
     4861 ggcttctcat ccggtggtgt cacggtctgc ccgcgcatca gttcagccgt gcgccctgca
     4921 tggagcataa tagctgaggc cagacggtct gaaatgacgc ccctgcgtgc ccatgagctg
     4981 aaatggctgt cacgctgtgt cgaaacaaaa ctgccctggg ttcgggatgc tgcaccgtaa
     5041 tacccgacag ccacaatatc cgggtcttcc gccggagcat tcgttggcgt gctctgtcca
     5101 ttctcatccg tcaggaacgg tacaaagaaa attttctgtg cttccttgcc tttgtagcca
     5161 ccgtatactg cctcatattt atcggtgcca gcgtttttcc agtaatacgt ggtatcaccg
     5221 caaatccacg gcaccgtggt ggcatttcca ctcacgcact gcgcagcaag cggagccagg
     5281 tcagttctga actgctgaac catggccgtg aataaactat tatgctgctg gcttcctgag
     5341 gcaaggtctg cctctccctg catccagacc acagccagaa gtttgttttt cgggtttttc
     5401 gccagcgcga ctttggtgcg gcttaccagg tcctgataca gaggtttacc ggccccccag
     5461 cgtgaagaat cagcggaagc accgctggcc tcactgaatg agccgtcggc tccagtggtg
     5521 aatgctgacc cgccacgaca acagggcacc agaagtatcc cggcattctg tgggatatag
     5581 ggaagcagtt ttttggcaat atgcaacccc tgaccgacag tcccgtactg gccttttgcc
     5641 agatcagctt tcgggtggtt tatcccgctc atatcctgaa catcatgcag gcagtggtct
     5701 gccggaatga tgtcgttgta tgtacacgac gtaccgttgg gcgttaccgt actgcggcgt
     5761 gccagttgct taatgcgtgg gtccgggcgg tcaaacgaat ccggtaacgg cagcccctcg
     5821 ccgtaggcca tgccatttga ctgacccgcg agaatgacaa caaagtaata ttccggctct
     5881 gaactgctgc cattcgtgct accggaaggc ccggcgttat ccggtgcatt ccagtcggag
     5941 ggcgtgagcg ttccggccac gtcagatgtg attggaatga tgccagtcac gcctgaactg
     6001 gtgatttctg cactgggatt accggatacc ggcattaccc ataatggctc tttcgtgctg
     6061 aagcggatca cgcagtctgc gaaggtgatc ccccctttat tacccgaagg gcggaaaggg
     6121 ctttcaataa atgccaccgt gccaccggca accttgactg aaaactctcc gggcatggcg
     6181 gagagcattt tccacttgga gtctgacatc gtcttttctg cctgtttctg aattaaacag
     6241 agtcagaata gcgaccgctg aaaaatatgt gttttgcgga aattcaaaaa agcgtcacgg
     6301 tacttttggt gacggagctg gtgaggcagg aaaataacga taaagggtgg aaaggccaat
     6361 atcaaatatc agtgcgattt gtttacggga ctcgccatta gccagcaaac gggcgatttg
     6421 ctcccgctct ttctctgtca gtttctgagg tctgccgcca tgacacccct gagcacgggc
     6481 agcagcaaga cccgcgcgtg tacgttcaat aatgagttcc cgctccattt ccgccagcgc
     6541 gcccattata tggaaaaaga aacgtcccat gggagtggat gtatcaatat tgtccgtaat
     6601 gctgcggaag ttaacgccgc gttgccggag ttcttctgtt aaaaggacaa ggtgctgcat
     6661 actgcgtcca aggcgatcca gtttccagac cagcagggta tcccctggcc ggagtcgctt
     6721 taatgcgcgt ttaagtccgg gacgctccct tgtcttaccg cttattctgt cttcgaaaaa
     6781 cagctcacat cctgcacatt ccagcgcatt tctttgtagc gccgtattct ggtcatttgt
     6841 tgacaccctg atatacccaa tcagcatacg atttccttat tataaaaagc cggaattgtc
     6901 gcatgaggaa taaaacagag ttaatcctgg ctttcctgaa aacctcggtt tacgggaaac
     6961 ggtaaacagg gcggcgaatg ctctgccatc ggacggcacc gccgttgccg cgaatagact
     7021 cgccacccca agaaatatta atggggttcc ctttgacggg acgcaggaca taaacatcac
     7081 ttctggtata acacagcagg atgcggattc ccgatatatc caggatatca ggctgggagc
     7141 agagaacagg caggtaatgt acggtggggc atattactct gcgtacggta atgttatggc
     7201 aggcctgcag attgacagta aagttgatgc ttcaaacgat tttatagcat tcagacccct
     7261 gcaaaaatta gtcggaggta catggattac ggtatctcag ttataaatag tcagtaaata
     7321 atatatttta aaacagattt atttccagga ggattttatg gaactaaaaa atataacccg
     7381 ttattatcct gaaaatatgc catatggtaa taatgttcag tatttccaga gtgaagatgg
     7441 taaggatttc tacgaatccc tgccattatt caccaaaaaa tataaacttt gcataacacc
     7501 tgacagcggc gttatctgct caatatcaca ggacgcctcg gcgctgtatc ctgcggggtt
     7561 ttctgtggtt gaggtcgatg aactccctga aggcacggat atttcaggaa actggaagtt
     7621 tgataatggc ataatctccc gtattccggt taactacgca agaaaactgg aagcaatgcg
     7681 ccagtcatat cttaatcagg catatgaaaa aattaatgac tggagaacgg agctacaact
     7741 gggcaccatc agcgatgaag acagagcagc acttacccaa tggatggcat acatcagtca
     7801 ggtgaagaaa atggaactac ccgccattaa aacagaagct gaatttaacg ccattaaatg
     7861 gcctgagcag ccacagtaat aaagaaggcc ggaaggcctt ctgaatatca ttatatactt
     7921 gcagccgtca cccatgctcc atttaacaga tactgcaacg gccggtaata cacgccgccg
     7981 acgttatctg ccgtattctt accgacatcc tgtactttaa ttccggtaag aacattgccg
     8041 ccccccagat tcagatccca gctttcccat gttccgggca tatgtgcggt caccgacccc
     8101 agtcttatgg cggttactgt tcctgatgag atagtgatat cctgcgtccc gtcaaaggga
     8161 acaccattaa ttgtgtgggc atttgccagc ctgttcgcgg caacggcggt gccgtccgat
     8221 ggcagggcat tcgccgccct gtttaccgtt tcccgtaaac cgacgttatc gataaaagcc
     8281 cctttatccg gaatgtctgc gccgttctgc tccctgcgca ggtaacggtt atcaccttcg
     8341 cttttgctgt aaacatccag attattacgg gcggttccct tattctccag gtcggcgaga
     8401 ttctgatcct tcgccagggc attaacgtca gcgggaccaa gactgttttt tgtggcaagc
     8461 gtccccagac caagataacc acgggccaga cgctgggcct ccgcaccggc atcagcaatt
     8521 tctttcaggt tatttttccg ggaaaaggca ttcgcagcga taatggcttt aatggacagc
     8581 gtaagctggt ttaactgcgc cttatccggc tgaatactgg cttcagccag aatattcagc
     8641 agttcggcct ggagaatatt cagccagtcc tgaccaatcc agctgatacc tttttgtccg
     8701 tcaccttcgg taaaccaggt ggtggtatta ctctgcgccg gtgccagcgc aggcatattc
     8761 gccacgccgg aattattatc aacatgaaac attaaatagt ctcctcttca tggctgtttg
     8821 cataaacgta aataaacgtc tgccatgcag gcttataacg gttcagaata cattccagcg
     8881 caccgccttc ataaatacgc agcggcgtga gaatatcatc cagcacattc atattccggt
     8941 agccggtttc actgttaatc gtgacaatac tgacccactg cgaatccggt gagggctgaa
     9001 tgtcaatctg ataaccaaac tccgccgcaa accggatata aaattcacgg ttcagcgagg
     9061 gcttcatccg gtatttattt ccggcataac gctgtcgctc cgtgagggtg gctccggcca
     9121 tatcacactc gggaagcccg agataacgct cccagtcttc cagcagcagg cgcgacgtgt
     9181 cagggaaacg ctcggcaagc agctgtttac ccgtccagga tacacgcgct gtcgaatgac
     9241 tgaggcccag acataatgat gccagtacgg atgacggacg cttgtcccat gccagcccgt
     9301 caggcagtaa ctgcaacagg gcgcgctgat gcggggtcag ggttacagcc atgtgatttc
     9361 tcctaccgtc agcagctcac tgtctcccgc ctgaactgac gtcagcggac tgcgcacttc
     9421 aaaatcctcc agatttttca cccctgccac cgcccgccag aaagacgacg gcagaaccag
     9481 accgccgggc cgggattcgt tatacaacag gtcggtcagc gcctgtttta cggccgcctg
     9541 attctccggt gttttcggga tgatcctgat gctgaagggc accggcttat tggtcagttt
     9601 aaacacggtc accgtcggcc ccagaggctg tccgacaggc tggccggtcg ccggatcatc
     9661 atgactgcgg atataatccg ccacccgctg aacatcaccg tcaccgggga aaatatccgg
     9721 gttattatcc
//
LOCUS       DAJTZL010000149         9644 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.149, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000149 DAJTZL010000000
VERSION     DAJTZL010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9644
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.149"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..667)
                     /locus_tag="OO544_003244"
     CDS             complement(<1..667)
                     /locus_tag="OO544_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HCR6768771.1"
                     /translation="MNITPFPTLSPATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIQ
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLNQAVERVHTAIVVQDPTMQRRTM
                     ATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVIFINQLQGLWPVERYLSLLTGELPR
                     LRDD"
     gene            complement(939..4841)
                     /gene="hrpA"
                     /locus_tag="OO544_003245"
     CDS             complement(939..4841)
                     /gene="hrpA"
                     /locus_tag="OO544_003245"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HCR6768772.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DSALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKIRKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQVMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDKLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            5042..5647
                     /gene="azoR"
                     /locus_tag="OO544_003246"
     CDS             5042..5647
                     /gene="azoR"
                     /locus_tag="OO544_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HCR6768773.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            complement(5698..7014)
                     /locus_tag="OO544_003247"
     CDS             complement(5698..7014)
                     /locus_tag="OO544_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HCR6768774.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDSNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSLFVCSTTFEQRRLVHRLILATVMACCG
                     FLLYPLKFSFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAVR
                     WRKVCGGWFLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKPDQRRIK
                     IALPYVVGACACIVLMELMMMVQLWWSVWLCWPVLSLLIIGRGYGGLGAITTGKDSQG
                     KLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKMTAGVYLGAFPRHIPAQNAVLDVTF
                     EFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALGLSRSA
                     LVVAAWLLCYGYCKTVDEAISFIRARRSHIVLKEDHKAMLKLWENR"
     gene            complement(7004..8761)
                     /locus_tag="OO544_003248"
     CDS             complement(7004..8761)
                     /locus_tag="OO544_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HCR6768775.1"
                     /translation="MENSRISGEHFFTTSDNTALFYRHWPALQPGAKKVIVLFHRGHE
                     HSGRLQHIVDELAMPDTAFYAWDARGHGKTSGPRGYSPSLARSVQDVDEFVRFAASDS
                     QVGLEEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALTLWHR
                     LRGLFFINSYVKRRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIIRDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFIRRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGT
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHVKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQE
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIEPGGILIYTGQPWHPQLELIAGVLTSHKDGKLWVMRVRTQGEMDLLVHDAGFDKCT
                     QRIDEWGIFTVSMAVRRDN"
     gene            complement(8777..9640)
                     /locus_tag="OO544_003249"
                     /pseudo
     CDS             complement(8777..9640)
                     /locus_tag="OO544_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005142702.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
BASE COUNT         2448 a         2627 c         2273 g         2296 t
ORIGIN      
        1 tatcatcgcg taaacgcggc agctcgccag tgagtagtga gagataacgc tcaaccggcc
       61 ataatccttg taactgatta ataaagatta cactgtctgc gttatttcct aactgaggaa
      121 cgaatccggg ataacttaac cagcgtggtg catcgggatt gtccccagtc atacggcgga
      181 acgtcgccat cgtgcgccgc tgcatggtgg ggtcctgaac aacgatagcc gtatgaactc
      241 gttctacggc ctgattcaat agcgcgatgc taaagcgtgc gttttcaccg cagtttgttg
      301 actggtcttc aatccagatt ttttcatgcg gaatgtgcca gaactgatga gcgatatccg
      361 cctggatggt cgcttctgct ctgccagtgg tgcggatagt gttgtagtgc ggatgctgtg
      421 cgatggcgct atacaaaaaa gttgtcgagt gaccgatacc accactaatc agtaaaggaa
      481 tttgctgatc gcgagcaatc ttacatgccg cgtcgatagt cggcataacc gcattgcctg
      541 caaggatcac gcaatcggcc tgatacggca cctcaccgga gaaatcatcc tgcgccagcc
      601 actgtccgat aacatttatg gcatctatag ttgccggcga aagcgtcgga aacggggtaa
      661 tgttcatggt tcctccttat cgttccctta agaatactgc gtgagataat agtgagaagc
      721 ggagcactct gaaaggagga agtcgtattt ccttaaccgc cactccgcaa gcctgacata
      781 tcttttaagg aaatgctgta gataaacatt attcatcttg cagcagagaa ttgtattttt
      841 ctgaattcct gaatttttac ccttaccgtt ttagaattaa taaaaaaccc ggcgctaaat
      901 gcttaccggg ttttttcttt atcaggcaaa tagcagggtt aaccgctaat ctgctccatc
      961 gcctgcaaaa tacgcttatc tgaaatcgga taaggcgtac caagttgttg agcgaagtaa
     1021 ctaacacgca actcttctat catccaacgg atctctttca cgtcttcatc ctcacgacgt
     1081 gcaggcggca gtttgttgat ccattgctgc cacgcctgct ggacgttttc gactttcagc
     1141 atctgagcac ggtcgcgatg tggatcaacc gccagttttt ccagccgttt ttcaatcgcc
     1201 tgcaaatatc gcagcgtgtc gcccagccgt ttgaagccgt taccagtgac aaaaccgcga
     1261 tataccagcc cgcccatctg cgctttaatg tcagaaagcc ccagcgccat cgtcatatcc
     1321 acccgcccct tcagacgttt gttgatattg aacactgccg taaggatttg ctcgacctgc
     1381 ttcgcaatat ccaccaccgt gtcgttcagt tcggcacgca ctttttcatg cagcgcagca
     1441 aagccttctt ccgtccagac cgggccacca ttggcgtcga tcaatttatc cacaccgcag
     1501 gagatacagt cgtcgatcag ctccagcact ttgccatacg ggttaaagta cagccccagc
     1561 ttggctttgt ttggtaactt ctcatgtaaa tacttgatcg gcgagggaat attcagcagc
     1621 agtagacggc gaagaccgtt ccacattacc tgtttttgct ccagcggatt atcaaacagt
     1681 ttgatcgcca cactgtcgcg ttcatccact agcgctggcc acgccttcac tttgtagttg
     1741 ccacgcttct gttcgtagct ttccggcagc tgaccaaaac tccagatatg cagtccgctc
     1801 tgctcgatac cgtcatccgc caccgcagac agcgtttcct gcactttgcc tttcagcgca
     1861 tctttcagat cttgtagcga gcgcccttct tttagcttct tgtttttgtc atccaccacg
     1921 cggaaggtga ttttcaggtg atcgggcacc tgatcccagt gccagtcttc gcggtcaacg
     1981 gtaacgccgg tcatccgccg taactcgcgc tcaaggctgt cgagcaacgg taactccagc
     2041 ggtgtgacgc ggcctaaaaa cgcttcggcg tagtttggtg caggcacaaa attacggcgt
     2101 accggtttcg gcaacgattt aatcagagca atcaccaatt cgcggcgcag accggggatc
     2161 tgccattcaa acccgctttc ctcaacctgg ttaagtaacg gcagcggaat atgtacggtc
     2221 acaccgtccg cgtccgcgcc tggctcaaac tgatagctca aacgcagctt gagattgccc
     2281 tgatgccaga agttcgggta atccagcttt ctgatttttt ccgccccttc tttgatcaac
     2341 atgctctttt caaagttgag caaatcaggc gtttcgcggc tgactttttt ccaccagcta
     2401 tcgaagtgac gagcggagat tacatcgtgg ctgatgcgct ggtcgtagaa ctcaaacagc
     2461 gtttcgtcat caaccagaat atcgcggcga cgtgatttgt gttccagctc ttccacttcg
     2521 gcccgcagtt tcaggttttc acggaagaat gcgtgacgcg tctgccagtc accttccacc
     2581 agagcgtggc gaataaagag ttcacgacat aacgccgaat cgatctggct gtagttgacc
     2641 ttgcgcgcag caacaatcgg caaaccataa acagtgactt tttccgttgc catcaccgcg
     2701 ccctgcgccc gttcccagtg cggttcgctg taggtgcgtt taatcaaatg ctgagcaacg
     2761 ggttctaccc attccgggtc gatacgcgca gcaatgcgcc cccacaggcg gctggtttct
     2821 accagttccg ccaccattac ccatttcggc ggctttttga ataaaccaga accggggaag
     2881 atggagaaac gcgcgttacg tgcaccggta tattcctgtt tatcggcatc tttcatgccg
     2941 atatgggaaa gcaaaccggt cagtaaggcg atgtgaattt cacgatactc cgccggttcg
     3001 ctgttgaccg gaatgccaag ttctttcacc acctggcgca actgggtgta gatatcctgc
     3061 cattcgcgca cgcgcagata gttgaggtaa tcggtacgac acaggcgacg gaaggcgttg
     3121 gaagaaagcg ccttttgctg ctcgccaaga taattccaca gatttacaaa cgcgaggaag
     3181 tcggattctt tgtcgtggaa gcgacgatgt ttttcgtccg atgcctgctg tttgtccatc
     3241 ggacgttcgc gcggatcctg aatggagagc gcggacgtga taatcatcgc ctcacgcacg
     3301 cagccatgtt tttgcgcttc cagcaccata cgcgccagac gtgggtcgac aggcaactgc
     3361 gagagctggc gaccgagcgg cgtcagttta taggcgctgg cctgttcatc cgtggtgatc
     3421 gcgcccagtt cttcgagcag acgcacgcca tcctggatat tgcgtttatc cggtgcttcg
     3481 acaaacggga acgcagcgat atcgcccagc cccagcgcgg tcatctgcaa aataaccgag
     3541 gccaggttgg tacgcagaat ctccggatcg gtaaactccg ggcgcgagag gaaatcgtct
     3601 tcggaataga gacgaataca gatcccttcg gacacacgac cacagcggcc tttacgctga
     3661 ttggcagacg cctgcgaaat gggttcaatc ggcaaacgct gcactttggt gcgatagctg
     3721 tagcggctga tacgcgctgt accggggtcg atgacgtatt taatccccgg tacggtcagc
     3781 gaggtttccg cgacgttggt cgccagcaca atgcgccgtc cgctgtgcga ctggaacacg
     3841 cggttctgtt cgctgttcga aagccgcgca taaagcggca agatttcggt atggcgtaag
     3901 ttcagcttgt tcagcgcatc ggcggtatcg cgaatttccc gctcaccact cataaagatc
     3961 agaatgtcgc cagggctttc ctgactcagt tcgtctacgg cgtcaaaaat cgcctgcaac
     4021 tgatcgcgct cggtgtcatc ggcttcttca acaatcggtc gatagcgcac ttccaccgga
     4081 taggtccgac cggagacttc gataatcggc gcattattaa agtggcgcga aaagcgttcc
     4141 gggtcgatag tcgcggaagt gataatgatt tttaggtcag gacgccgcgg cagcaactct
     4201 ttcaaatagc cgagcagaaa atcgatattc aggctgcgtt cgtgcgcttc gtcaataatg
     4261 atggtgtcgt actgcatcag caggcggtct tgctggatct ccgccagcag gataccgtcg
     4321 gtcatcagct tgaccatcgt gttatcactt acgtgatcgc tgaaacgcac tttgtaaccg
     4381 atgcaaccgc ccggctccgt tttcagctct tccgcaatac ggttcgccac cgttcttgcc
     4441 gccaggcgac gcggctgggt atggccgatc agccctttaa tcccgcgccc cagctccata
     4501 cagattttcg gtaactgagt cgttttacca gaacccgttt ccccggcgac gatcaccacc
     4561 tggtgatcac gaatcgcttc gagaatgtcc tgttttttct gactaaccgg taaattgtca
     4621 ggataagtaa tttccggtcg tgccgcttca cgcagcagga ctttccctgc cgcctggtca
     4681 atctctttcg ccatctcctg gaaaatggcc tgttgtgcat caggattttt aaccttcttc
     4741 acgccgtgca gacggcgaga aaaacgcagt ctgtcacgca gcatcagcga atccagccgc
     4801 tgctgcaagg ccgtaaaggt caatttttgt tgttctgtca taacgttagt gggcagtagt
     4861 ctgcctgatt aattttcttt gaaatgatag atatagagta ccacatcacg gctttctatg
     4921 cgttgttcaa taaattcgaa cataggcttc gatttattgc gctatctctg tggcaggatt
     4981 gtgaataaag tgtcaacaag caacggggca tcgcccattc aaacatctat aaggaaacac
     5041 catgagcaag gtattagttc ttaaatccag catcctggca gggtactctc agtctaatca
     5101 gttgtccgat tattttgttg aacaatggcg cgaaaagcac tccgctgatg aaatcaccgt
     5161 tcgcgacctg gctgcaaatc cgattccggt actggatggc gaactggttg gtgcactgcg
     5221 tccgagcgat gcgccgctga ctccgcgtca gcaggaagct ctggcgcttt ccgatgagct
     5281 gattgccgag ctgaaagccc acgacgttat cgttattgcg gcaccgatgt ataacttcaa
     5341 catctcaact cagttgaaaa actattttga cctggttgca cgcgcaggcg ttactttccg
     5401 ctataccgag aacggtccgg aaggtctggt aacgggtaaa aaagccatcg ttattaccag
     5461 ccgcggtggg atccacaaag atggaccaac ggacctggtg acgccgtatc tgtccacgtt
     5521 cctcggcttt atcggcatta ctgatgtgaa atttgtcttc gccgaaggga tcgcatacgg
     5581 tccggaaatg gcagcgaaag cgcagtctga cgcgaaagca gccatcgaca gcattgttgc
     5641 tgcataaccg gacacgatct tgatgtggac tgtagtccag tccacatccc aatccactta
     5701 cctgttttcc cataatttca gcatcgcttt gtgatcttcc ttaagcacaa tatgcgagcg
     5761 tctggctcga ataaagctaa tcgcttcatc aacggtttta cagtatccgt aacataacag
     5821 ccatgccgcc actaccagcg cactgcgcga taatcccagt gcgcaatgga ccagaacgct
     5881 gccttgctct tcgcgtaatg tttccagcat cgccacggcc tgtcggagct ccccctcttc
     5941 cggaaccacc agatctagca tcggtacaca gaaatagagt cgatcttttg tcgctcgtcc
     6001 acgagggaat tcaaaggtga cgtccagaac cgcattctgt gccggaatat gtcgtggaaa
     6061 cgcccctaaa taaacaccag cagtcatttt gctcaccggc tccaggcgac gacaaaacca
     6121 acgcatagat agccacatcc cgatgcgcca gggcaatgtc agccagtaaa cggcgggcgg
     6181 gagtttcccc tgactatctt tccctgttgt tatcgcgcca agcccaccgt acccacggcc
     6241 aataatgagt agcgataata ctggccaaca taaccagact gaccaccata actgaaccat
     6301 catcattagc tccatcaaca caatgcacgc gcacgcgcct acgacatatg gcagtgctat
     6361 tttgattcga cgttgatcag gtttctgata attccaacga cggtcgacgg gcaccatcca
     6421 gtcaatcaac atacctaccg ccagccctgt gatgacatca ataaaatgat gctgccatgt
     6481 cgtcagcgtc gaaatggcga tgagtaaaaa ccatccgccg cagactttac gccacctcac
     6541 agccagatgc tgacgaaagt gacgccaaag tagccagcag agaataatat gcagcgaagg
     6601 agactggtta taaggcagat caaacagttc aagttgcgaa aatagccatc ccgtcacccc
     6661 actcacttca ggacggataa aactaaactt cagcggatag agcaaaaaac cgcagcaggc
     6721 cattaccgtt gccagaataa gccggtggac aagtcggcgc tgttcgaatg tcgtgctaca
     6781 aacgaacagc gaaaatccat ataaaagatc cagactccag taaggaacaa tagtccaggg
     6841 aagaaaaggg atcgccgttt cccaaccgaa tacctgactg ggaatatcat ggctattact
     6901 gtcctgaacc gcggtgaact gattaagaga tccataggtg aagaagaaaa acggggccag
     6961 caacaataac cagccagctc cttgtagcaa tacgttccgg cgttcagtta tcacgacgca
     7021 ccgccatcga aaccgtaaaa atgccccact catcaatccg ttgtgtgcat ttatcaaatc
     7081 cagcatcatg cacgagcaaa tccatctccc cttgcgtacg cacgcgcatt acccacagtt
     7141 taccatcttt atgactggtt aacaccccgg caatcagctc cagttgtggg tgccacggct
     7201 gcccggtgta gatgagaatg ccacccggtt cgatggcatt ggcaagacct gcgagtgagt
     7261 tttttacctg ctcattttcg ggaaacagct catacagtcc agagacaatc gccagcgtag
     7321 gccgcggagt taacgcgctg agttcctccg ggttaaacgc atcgccctgt tcaaaacgca
     7381 cccgcccaga cattccccgt tgggcaatca tctcttgccc ctgtgcaaca tttaactcac
     7441 tgtaatcacg taataaaata tcgcttacgg caggctcgtt tgccagtgca tccagtacat
     7501 agcgcccatg ccctgcggca atgtcaacca cgcggacggc taaacctttg acgtggagat
     7561 cggcaacggc ttgtttaatc agtatttgta aatgagtttt gcgctggcga ataccgcgcc
     7621 agccaacact gttcaggtaa attttgtcga ttaagcgccc gaatgcgtta ctgccttgcg
     7681 gttgattttg atagacataa tccagcgaac tgcctgaatc aaagccagtg ctcattccga
     7741 gatgcaggcc cgcagagtgc gtcccgaaca atttcatcgc tttacgcata aagcgatacg
     7801 ccaaatcaac cggcgataat ggcacgggtc cacctgaaag gaggcgccag cgatccgctg
     7861 atggtccagt gcggtcttca tgctgataat caaatttttg tgacttgtta gcatataaac
     7921 gacgaataaa gctttgcatt ttttcaaatg cctgcgccct gttctcttca cccaacgtgt
     7981 cgtgataaaa gcctggcagc agatgcagct ctttcagagg gctacgtaat ctctgataaa
     8041 aatcaatctg cggttggcga tgcaccacat agtcatcgcc tgatatcaga agttgcgtgg
     8101 ggagcgtaat cgccgccgca tctctaataa tacgttcaga cgttttatag agatcgagca
     8161 agatattgac ggcaatcgcc cgtgtgatca gcggatcatt attgaaactc gccacccgtt
     8221 gccgatcgtg ggtcaaatag cgtcttttca cataggaatt aataaaaaac agaccacgca
     8281 gacgatgcca tagcgtcagc gcaggacgtg ccagcggcac atacaattta accttaaagg
     8341 ccggagaagc cagcaccagc ccgcgaattg caggcgcata atcatgaacc catgtggcaa
     8401 ccagcactgc gccgacgctt tgcgcgatca caaccacctc ttccagtcca acctggctgt
     8461 cgctggcagc aaaacggaca aattcatcga catcctgcac tgaacgcgca agagatgggc
     8521 tataaccacg cggccccgaa gtttttccat gcccccgggc atcccatgca taaaaagcag
     8581 tatctggcat cgccagttca tcaacgatat gttgtagacg accagaatgt tcatggccac
     8641 gatgaaataa gacgatgacc tttttcgccc ctggctgtaa agcgggccag tgccgataaa
     8701 acaacgctgt attatcactg gtggtaaaaa aatgttcccc agagatgcgt gaattttcca
     8761 ttttctctat tttccttcag taacagcagt aacgaataaa ataaaaaaat accggagcgg
     8821 tgaaaattaa tgagtcaatc ctgtcaagga ttccgccatg tccaggtaat agtttcccgc
     8881 tatctttaac accaacatct cgtttgatgg ctgacatcac tacatcgccg caaaaaccac
     8941 taataccaat taacaaaccc gctaatattg cctgtaatgt atttagcggt gtcagtaacg
     9001 gtccaatgat catcgacgct atcatggtgg tgattacgcc gccgaccaga ccttccagcg
     9061 tttttcccgg gctgactttg gggaccactt ttcttctgcc gcaggatttc ccccataaat
     9121 actgtgcaat atcgtttgac gcggttaatg ccaaaagaaa taacaccagt aaggccccgc
     9181 cctggatatt catggatggc aacattaata actaggcggc atgactcaag gcgaaaacgg
     9241 tcattatcca gccccaaaaa atggcgctca cggtatgcag aaaaccggag gggtcaccca
     9301 caaaaactcg ccaggcggct aatatcagaa acccagccaa aggtataaat aacagaaaca
     9361 gctcaaagca attaaaaccg ataagcaaat agttaagagg aataacccaa tataaccacc
     9421 gcggaaaatg tacggatata agcatacaat attctttcaa tgccagaaaa cttatcagcg
     9481 caaaaaacgc caacgtcatc cagcgtggac ctgaaatcac cagtgaaaaa caaatgacga
     9541 tgccccacca ggtacgaata cgtaatgtaa cctcaccccc ttttctctcc ggtaaccgcc
     9601 atagcagaaa acggtttatc agcgttgcta aaattaacaa cgcg
//
LOCUS       DAJTZL010000150         9612 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.150, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000150 DAJTZL010000000
VERSION     DAJTZL010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9612
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.150"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            223..2199
                     /locus_tag="OO544_003250"
                     /pseudo
     CDS             223..2199
                     /locus_tag="OO544_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311934.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2192..3328
                     /locus_tag="OO544_003251"
     CDS             2192..3328
                     /locus_tag="OO544_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HCR6768776.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSGGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDMLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAVEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            3325..5325
                     /locus_tag="OO544_003252"
     CDS             3325..5325
                     /locus_tag="OO544_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HCR6768777.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     DALIATFSDGVRTQLANGQALKEAQCSCGANGMCRHRVMLVLSYQRLCATTQSTEKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKGITIELFCAPGEIPSARLPMSDMRFYSRSS
                     IRFARCDCIESTLCEHVVLAVQAFVQAKAQQAELTHLIWQMRSEHVTSSDDPFASEEG
                     KTCRQYVQQLSQALWLSGISQPLIHYEAAFSRAQQAAERCSWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNSRLNCAQEMARRDSIGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQENSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASACSPFAVERMAALLQQTDDPVSLVSGFVSFVEGQLTLEPRVMMTKTRAWALDAET
                     APVAPLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAISQAELLANDLSAV
                     GFYRLAHVLGQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            5447..5908
                     /locus_tag="OO544_003253"
     CDS             5447..5908
                     /locus_tag="OO544_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HCR6768778.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKLTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(5949..6419)
                     /locus_tag="OO544_003254"
     CDS             complement(5949..6419)
                     /locus_tag="OO544_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HCR6768779.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(6466..7185)
                     /gene="yehT"
                     /locus_tag="OO544_003255"
     CDS             complement(6466..7185)
                     /gene="yehT"
                     /locus_tag="OO544_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HCR6768780.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(7182..8867)
                     /gene="btsS"
                     /locus_tag="OO544_003256"
     CDS             complement(7182..8867)
                     /gene="btsS"
                     /locus_tag="OO544_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HCR6768781.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISSP
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            9089..9154
                     /locus_tag="OO544_003257"
                     /pseudo
     CDS             9089..9154
                     /locus_tag="OO544_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
     gene            9382..>9612
                     /locus_tag="OO544_003258"
     CDS             9382..>9612
                     /locus_tag="OO544_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HCR6768782.1"
                     /translation="MRVEICIAKEKITKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESAD"
BASE COUNT         2353 a         2531 c         2464 g         2264 t
ORIGIN      
        1 cggccacgat acgtattgat tgaaggcccg gctgatttta atgaccgggt agacgaactg
       61 tttttagccc accagcttcc ggtagctatt tacagttatt gccagtatca ggacggtgca
      121 gcccccgggc gtggtgcctg gacgccattt gctgaatttt cgccggagtg gcaggcgcta
      181 caagccgcac gtcgtattca ggcacaaact tacttcatcg atttgccttg ctgggcgcag
      241 agtgaagaag tggacgattc gcctgatacg caagaggaaa gccaggcctt gctgctgcgt
      301 gccacccgca tggataacag cgacaccctg tgggatcact tgttcgaaga tgaaagccag
      361 caaactgcat taccctctgc actggcgcac tattttgccc aactgcgggg cgattccccc
      421 ggcgatgcac tcaatcgtca gcgcgaagcc tttatggccc gctggattgg atgggcgatg
      481 cagcaaaaca atggcgacgt gttagtcgtc tgcggtggct ggcacgctcc tgcactggca
      541 aaaatgtgac gcgaatgccc gcaggacatt aacacgccgg aattgccctc gctggcagat
      601 gccgttacag gttgttatct caccccctac agtgaaaagc gccttgatgt gctggcagga
      661 tacctttccg gtatgcctgc cccggtctgg caaagctggt actggcagtg gggcttacag
      721 caggcgggag aacaactgct aaaaacggtt cttacccgtt tgcgccagca caaattgcct
      781 gcttcgaccg cggatatggc tgccgctcat ctgcatgcga tggcgctggc acagttgcgc
      841 ggacataaac taccgttacg cactgactgg ctggatgcca tagcaggctc gctgattaaa
      901 gaagccctga acgcgccgtt gccgtggagc tatcgcggag ttattcatcc cgacaccgat
      961 ccgattctgc taacgttgat agacacatta gcgggtgacg gattcggtaa acttgcccct
     1021 tctacgccac aaccgcctct gccaaaagat gtcacctgcg aactggaacg taccgcaatc
     1081 tctcttccgg cggagcttac cttaaatcgc tttaacccca atgggctagc gcaaagtcag
     1141 gtgttacatc ggctggcaat actggagatc ccagggattg tacgccagca gggaagcaca
     1201 ctgacacttg caggcaacgg tgaagaacgc tggaaattaa cccgcccgct tagccagcat
     1261 gcggcattga ttgaggccgc ctgctttggt gccacactcc aggaagccgc acgccataaa
     1321 ttagaagccg atatgctgga cgcgggtgga atcggcagta tcaccacatg tcttagccag
     1381 gcggcgttag cgggtctggc gtccttcagt caacaattac tggagcaact cacattatta
     1441 atcgcccagg aaaatcaatt tgccgaaatg ggccaggcgc tggaagtgct atatgcctta
     1501 tggcggctgg atgaaattag cggtatgcaa ggcgcgcaga tattacaaac gacgttatgc
     1561 gcggctatcg atcgcacgct gtggctgtgt gaatctaacg gcagaccaga tgaaaaggag
     1621 tttcacgctc acctgcatag ctggcaggcg ctttgccata tcctgcgcga tctacatagc
     1681 ggcgttaatt tacccggcgt ttttctttct gcggcggtag ccttactgga gcgccgcagt
     1741 caggcgattc atgtcccggc gctggatcgc ggcgcggctc ttggcgcact gatgcgtctg
     1801 gaacatccca acgccagtgc cgaagcggcg ctgacgatgc tggcgcagtt atccccggca
     1861 caatccggcg aggcgctgca cggtttgctg gcgctcgccc gtcatcaact ggcctgccaa
     1921 ccagcattta tcgccggttt cagtagtcat ttaaatcaac tgagtgacga cgattttatt
     1981 aacgccctgc ccgatttgcg cgcggcgatg gcctggctac cgccacgaga acgcgggaca
     2041 ctggcgcatc aggtgcttga gcattaccag cttgcgcagc ttcctgtttc agcgctgcaa
     2101 atgccgttgc attgtcctcc gcaagccatt gcacatcatc aacaactcga acagcaggca
     2161 ctggcatcgc tgcaaaactg gggagttttc catgtctgaa ctgaacgatc ttctgaccac
     2221 ccgtgagcta caacgctggc gattaattct tggcgaagcg gcagaaacga cgctttgtgg
     2281 gctggatgac aacgcccggc agatagacca cgcgctggaa tggctgtatg ggcgcgatcc
     2341 tgaacggctc cagcgtggtg aacgctccgg tggattaggt ggctcaaatc tcaccactcc
     2401 tgagtggatc aacagtattc acacgctgtt tccgcaacag gtgattgagc ggctggaaag
     2461 cgatgccgta ctgcgctacg gcattgaaga tgtggtgacg aatctcgaca tgctggaacg
     2521 tatgcagcct tctgaaagcc tgctacgcgc cgttttgcac accaaacatc tgatgaatcc
     2581 cgaagtactg gctgccgccc gccggatagt gcgccaggtt gttgaagaaa ttatggctcg
     2641 actggcaaag gaagttcgtc aggctttttc tggtgtccgc gatcgccgtc gccgctcatt
     2701 tattccactg gcgcgaaact ttgatttcaa aagtactctg cgcgctaacc tgcaacactg
     2761 gcacccgcaa cacggcaagt tgtatatcga atccccccgc tttaacagcc gcattaaacg
     2821 ccaaagcgaa caatggcaac tggtcttact ggttgatcaa agcggatcga tggtggactc
     2881 ggtgatccac tctgcggtga tggcggcctg tttgtggcag ttacccggca ttcgtaccca
     2941 tctggtggcg tttgacacaa gcgtggttga tctcacggca gacgttgccg atccggtaga
     3001 gttattaatg aaagtacagt tgggcggcgg gaccaatatc gccagtgccg tggagtatgg
     3061 tcggcaactt attgaacaac cagcaaaaag cgtcattatc ctcgtgagcg atttttacga
     3121 agggggttca tcatcattgc tgacgcatca ggtgaaaaag tgtgtccaga gcggcatcaa
     3181 agtgctggga ctggctgcgc tcgatagcac cgcaacacct tgctatgacc gcgatacggc
     3241 ccaggcgctg gttaatgtcg gcgcacaaat agccgccatg acgccgggcg agctggcatc
     3301 atggcttgcg gagaatcttc agtcatgaat tcactacgtc cggaattatt agaactgaca
     3361 ccgcaggccc tgacggcgtt aagcaatgcc ggttttgtta agcgcagtct taaggaactg
     3421 gaaaatggca acgtcccgga gatcagccat gagaacgacg ctttaatcgc caccttcagt
     3481 gacggtgtcc gtacccagct ggcgaacggc caggcactga aagaggctca gtgcagttgc
     3541 ggggccaacg gtatgtgccg tcatcgcgtg atgctggtgt taagttatca acgactttgt
     3601 gccaccactc agtctacgga aaaagaagaa gagtgggatc cggcaatctg gctggaagaa
     3661 ctggctaccc ttcccgatgc tacccgcaaa cgcgcacagg cgctggtcgc taaaggcatc
     3721 accattgagt tgttctgtgc gccgggtgaa attccctctg cccgcttacc gatgagcgat
     3781 atgcgttttt attcccgcag cagtattcgt ttcgcccgtt gtgattgtat tgaaagcaca
     3841 ctttgcgaac atgtcgtact ggcagtacag gcctttgtcc aggcaaaagc gcagcaagcg
     3901 gaattgactc atttaatctg gcagatgcgc agcgaacacg tcacatcatc tgacgatccg
     3961 tttgccagcg aagaaggcaa aacgtgtcgt caatatgttc aacaactaag ccaggcatta
     4021 tggctgagcg gcatcagcca gccgcttatt cactacgagg ccgctttcag ccgcgcgcag
     4081 caagcagcgg aacgctgcag ctggcgatgg gtgagtgaat cgctacggca actgcgcgcc
     4141 agcgttgatg ccttccacgc ccgcgccagc cactatcatg ccggagaatg cttacgtcag
     4201 cttgcggcat taaacagtcg attaaattgc gcacaagaga tggcccggcg cgacagtatt
     4261 ggtgaagttc ctcctgtgcc gtggcgcacg gtcgttggct ctggcattgc cggagaagca
     4321 aagcttgatc atctgcggct ggtgtcttta ggtatgcgtt gctggcagga tattgagcat
     4381 tatggtttac gcatctggtt taccgatccc gacaccggca gtattttgca cctttcgcgc
     4441 agttggccgc gaagtgaaca ggaaaactca ccggcagcta cgcgtcggct gtttagtttt
     4501 caggctggcg cactagcggg cgggcaaatt gtttcacaag cagcaaaacg cagtgccgat
     4561 ggcgagctgc tgttagctac ccgcaaccgc ttaagcagcg ttgtgccgct gtcgcctgat
     4621 gcctggcaaa tgttgagcgc gccgttacgc cagccgggca ttgtggcttt gcgggaatat
     4681 ttacgccagc gtccccccgc ctgcatacgg cctcttaatc aggtcgataa cttatttatt
     4741 ctgccggtcg ctgagtgtat ttcgctcggt tgggatagca gccgccagac gctggatgcg
     4801 caggtaatca gcggcgaagg tgaagataat ctcctgacgt tatcccttcc ggcatctgcc
     4861 tgttctcctt ttgccgttga acgcatggcg gcgcttttgc aacaaacaga cgaccccgtg
     4921 agtctggttt ctggctttgt cagctttgta gaggggcaat tgacactgga accacgggtg
     4981 atgatgacaa aaacccgcgc ctgggcgctg gacgcagaaa ctgcgcctgt ggcaccgcta
     5041 ccttctgcca gcgttttgcc tgtgccgtct accgctcatc agttgctgat gcgctgccag
     5101 gcgttactta ttcaactgct ccataacggc tggcgctatc aggaacagag cgctattagt
     5161 caggcagagt tgctggcgaa tgacctctcc gcggtcggtt tttatcggct ggcacatgtg
     5221 ttgggacaat ttcgtaatac agaaagcgag gcacgggtag aagcaatgaa taacggtgtt
     5281 ttactttgcg aacaattatt ccccatgctt cagcaacaag gatgaaatag tgcttactaa
     5341 gagttctact ccagttccgg actgctcacg ccacggtatt aggcatatcc tatatagccc
     5401 ctggtgagag tcaccagttc cttgattaaa taaaatggag ttttacatga aggctttcaa
     5461 taagctgttt tccctcgttg ttgcatctgt tctggttttc tctcttgctg gctgcggtga
     5521 caaagaagaa tcgaagaaat tcagcgccaa tctgaacggc actgaaattg ccattaccta
     5581 tgtctacaaa ggtgacaagg tgcttaagca atcttctgaa accaaaattc aatttgcctc
     5641 cattggtgca accaccaaag aagatgctgc caagacactt gagccgttaa gcgccaaata
     5701 caaaaacatc gcgggtgttg aagaaaaatt aacctatacc gatacctacg cgcaggaaaa
     5761 cgtgactatc gatatggaaa aagtggattt taaagccctg cagggtattt caggaatcaa
     5821 cgtttctgct gaagatgcca aaaaaggtat cactatggcg caaatggaac tggtgatgaa
     5881 agccgctggt tttaaagaag tgaaataatc tgtcggcggc catgtttcgc atggccgcca
     5941 tacccgtctt agcttttctt cacatgctgg cgcgctgcca gtccacgcag aaaataacgt
     6001 aaaaattgat cgccgcattc gcggaagttt ttatgatccg gtgcgcgcat catcgctgta
     6061 atttccggca tcgaaacgcg gaactgctgt tcggtgagga tcgccagaat gtcatcggtt
     6121 ttcagcgaaa acgcgatgcg taattttttc agcacgatgt tgttattaat gcgacgttcc
     6181 ggctccagtg ccggagcaga ctcatccttg ccgcgttttt cataaatcag gccattgagg
     6241 aatgacgaca aaacaatgtc cggacaacgc tgaaaaccct cttcgtcttc tttacgtagc
     6301 cagacggcga tctgttccgc ggtggcttcg acattaccca gcgccagaat acgcaccagg
     6361 tcattattat tggctttcaa aatgtagcgc acgctgcgca gaatatcgtt acttagcatg
     6421 aggccttcag gtgttaatga ggcaaaaagc cattttagca gtcttttaca ggccaatcgc
     6481 ctcttttaag cttttcagat aacggcggct gaccggcacg gttaagccat tacgcaaaat
     6541 caactcggcc tggccgttat cttccagacg aatctcctgt aaatgcgcga ggttaaccag
     6601 atactgacga tggcagcgca gtagtggtgt acgactttcc agggtacgta atgtcaattc
     6661 ggtaaagccc tctttccctt cgtggctggt aacgtagaca ccgctcatcc gactgctgac
     6721 aaatgccaca tctttcattt gcagcaaata aatccgacta tgccccgtac aagggataaa
     6781 tttcagcgcc tgttgatttt ccggtaacag cgaaacatcc tgcttgctgc gctcctgacg
     6841 caatcgcgcc agcgttttct ccagtcgcgc ttcatcaatt ggcttcagca gataatcaaa
     6901 ggcatgttct tcaaaggctt taatagcgta ttcgtcaaac gcagtgagaa aaacaatata
     6961 cgggcgatgt tccgggtcaa gcatccccac catttccaga ccactgatgc gcggcatctg
     7021 gatatcgaga aacagcacat ccgggcgcag tttatgcacc gcgccgatcc cttccacggc
     7081 gtttgaacac tctccaacga tttcaatatc gctctgctcc tgcaaaaata cacgcaggtt
     7141 ctcccgtgct aacggttcat catcgacaat taagacttta atcatgcctc gtccctccat
     7201 ggtagtcgta acgttattcg ggtgtaacta tcaggctcac aggcgacgct tattccatag
     7261 tcatcgccaa accgttcacg taaacgctta tccaccagat tcatccccag cccactggca
     7321 ttggttaccg gttgatacaa accggcattg tcttcgatct ccagcatcaa atgttgcccc
     7381 tcacgtcggg cgctgattgc cactcgccct gtatccagca gttgtgatgt cccatgttta
     7441 atggcgtttt ccactatcgg ttgcagggta aacgcgggca attgctgctg ggataattct
     7501 tgcggaatag caatgttgac ctgcaaccgc gactggaagc gcgccttttc aatttgcaga
     7561 taagcgttca catgttcaat ttcgtcggcg agagtaacaa actccgaagg ccgctttaag
     7621 tttttgcgga aaaaagtgga aagatactgc accagctggc tggcctgttc gctgtcgcgg
     7681 cggatcaccg ctttaatggt gttaagcgca ttaaacaaaa aatgaggatt cacctgggcg
     7741 tgaagcagtt tgatttctga ctgggtgagc atcgcttttt gccgctcata ttgcccggca
     7801 agaatctgcg ccgaaagcag ttgcgcaatc ccctcgccca gcgtgcggtt tattgaactg
     7861 aataaacggt ttttggcttc atacaatttg atggtgccca tcacgcgctg attttcgcca
     7921 cgcaacggaa ttaccagcgt cgaccccagt ttgcattgcg gatgcaaaga gcaacgataa
     7981 ggtacttcgt tgccatcagc gtagaccact tcaccggttt caatcgcttt taaggtgtaa
     8041 ggcgaagaaa tcggtttgcc gggtaaatgg tggtcgtcac caattccggt aaaggccagc
     8101 aatttctctc gatcggtaat cgcgactgca ccaatatcca gctcctgata cagcacctga
     8161 gccactttca tgctgttcac ttcgttaaac ccctgtcgca aaatgccttc cgtcgaggct
     8221 gccactttca gcgcagtggc agaaaaagcc gaagtgtatt tttcaaacat cgcgcgttta
     8281 tcgagcaata tacgcataaa cagcgccgcg ccgacggtat tggtgaccat cattggcgca
     8341 gcaatattac tcaccagacg caccgcatct tcataaggtc gggcgatcgc caggatgatc
     8401 agcatttgca ccatttcagc gacgaacgtg acggcaccgg cggtaatggg gttaaagact
     8461 ttatcagtgc gcccgcgacg gatcaggatg ctgtgtacca ggccgccgag caatccttca
     8521 acgatggttg agatcatgca acttagcgcg gtcatgcccc ccatcgaata tcgatgtaag
     8581 ccgccggtca gaccaaccag cccaccgacg accggaccgc cgagtaagcc gcccattacc
     8641 gcgcctatcg cacgggtatt ggcaatagaa tcgtcaatgt gcaacccaaa ccaggtgccc
     8701 atgatgcaga agatggaaaa gacgatgtag cagagaaatt tatgcggcag acgaaccgtg
     8761 acctgcatta acggtatgaa taatggcgtt ttactcatta accacgcaat gactaaaaaa
     8821 acgcacatct gctgaagcag cagcaacacc agattaaaat cgtacatacc cgcaaaccac
     8881 acttcccttt aaaacgcgta acatacattg cctgcgttta actttctttg aactcttgca
     8941 gaaaaatgag aattcgtgag tacgatcact caaaatcgcc tggcaaaaat aaaatcaccc
     9001 tatagatgca caaaaaacgg gcaaaactac ctggttcgca aaactgcgtc taaagttaaa
     9061 ccgggacctc gcgagcaagg gtgagacgat ggcgctttac acaattggtg aagtggcgtt
     9121 gctttgtgat attaaccctg tcacgttacg cgcgtgacgc aacattttta aaaatcgacg
     9181 aagatcgtcc aggagcgccg tttttcaagg gttggataga tgattcgttt accatgtcga
     9241 cgaacttcga cgaatttcgc cgataaaaag tgtcccatac atgccccatt gatgccccat
     9301 aaacaatatc aggaattgac actggttatc catacagata aaaataacac ctgtacataa
     9361 ccacagttac aggagggtgt tatgcgtgtt gagatctgta tcgctaaaga aaaaatcact
     9421 aaaatgccaa acggtgctgt ggatgcgtta aaggaagaat taacccgacg catcagtaaa
     9481 cgttatgacg atgtagaggt gatcgtaaaa gccaccagca acgatggcct ttctgttaca
     9541 cgcaccgcag ataaggattc tgcaaaaaca tttgttcagg agactctgaa agatacctgg
     9601 gagtctgctg ac
//
LOCUS       DAJTZL010000151         9574 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.151, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000151 DAJTZL010000000
VERSION     DAJTZL010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9574
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.151"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..158)
                     /locus_tag="OO544_003259"
     CDS             complement(<1..158)
                     /locus_tag="OO544_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 family protein"
                     /protein_id="HCR6768783.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPA"
     gene            complement(158..1084)
                     /gene="glsB"
                     /locus_tag="OO544_003260"
     CDS             complement(158..1084)
                     /gene="glsB"
                     /locus_tag="OO544_003260"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HCR6768784.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(1148..2536)
                     /gene="sad"
                     /locus_tag="OO544_003261"
     CDS             complement(1148..2536)
                     /gene="sad"
                     /locus_tag="OO544_003261"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HCR6768785.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGANDIENALELAAAGFRD
                     WRETNIDYRAEKMRDIGKALRARSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNDGVSQMIKDSRIAAVTVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMGDPRDEENTLGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITVAKDAEHALELANDSEFG
                     LSATIFTTDETQARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRI"
     gene            2637..3518
                     /locus_tag="OO544_003262"
     CDS             2637..3518
                     /locus_tag="OO544_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6768786.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAKVHRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNHRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVIRASQVNGSNIYAFRTNCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLE
                     AFIQLLDVSDSAKQSYQ"
     gene            3595..4710
                     /gene="yneK"
                     /locus_tag="OO544_003263"
     CDS             3595..4710
                     /gene="yneK"
                     /locus_tag="OO544_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HCR6768787.1"
                     /translation="MVTPVSISNYISLPDDFPVRNIAPQVKEVLKDFIDALSTIICDE
                     EWRTSLNINSATKKIFNNLDNLSYIQRTSFRGNDTLYNEKVQFKLTYPVKNGRHKENI
                     EFQVVINLSPIYLDNFRHDGEINIFCAPNPKPVTMGRVFQTGVERVLFLFLNDFIEQF
                     PMFNPGVPIKIAHTPHIEPLPPDHHTAADYLRQFDLLVLNFISRGNFVILPRLWNNSE
                     VHRWFVNKDPNLITAILDITDSELKEDLLQSLMDSLGSNKHVLPEVCICFLFLLAEQE
                     SPHFQDLFLFFANMLLHYHQFMNPNESDLNDVLMPASLSDDKIIKHMARRTLELFVKN
                     ETPPKVTHEDLVKNRPRSPVRPPIPATSKTPDLPERH"
     gene            4860..6050
                     /gene="ydeA"
                     /locus_tag="OO544_003264"
     CDS             4860..6050
                     /gene="ydeA"
                     /locus_tag="OO544_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HCR6768788.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(6075..6740)
                     /gene="marC"
                     /locus_tag="OO544_003265"
     CDS             complement(6075..6740)
                     /gene="marC"
                     /locus_tag="OO544_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HCR6768789.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            6952..7386
                     /gene="marR"
                     /locus_tag="OO544_003266"
     CDS             6952..7386
                     /gene="marR"
                     /locus_tag="OO544_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HCR6768790.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            7406..7789
                     /gene="marA"
                     /locus_tag="OO544_003267"
     CDS             7406..7789
                     /gene="marA"
                     /locus_tag="OO544_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HCR6768791.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            7821..8039
                     /gene="marB"
                     /locus_tag="OO544_003268"
     CDS             7821..8039
                     /gene="marB"
                     /locus_tag="OO544_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HCR6768792.1"
                     /translation="MKPLSSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(8096..9535)
                     /locus_tag="OO544_003269"
     CDS             complement(8096..9535)
                     /locus_tag="OO544_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCR6768793.1"
                     /translation="MSGFKKGFLWGGAVAAHQLEGGWNEGGKGISIADVMTAGAHGVP
                     REVTEGVIDGLNYPNHEAIDFYHRYKTDIQLFAEMGFKCFRTSIAWTRIFPQGDEQEP
                     NEEGLQFYDDLFDECLKQGMEPVVTLSHFEMPYHLVTKYGGWRNRKLIDFFIRFASTV
                     FTRYKEKVKYWMTFNEINNQVNFSESLCPFTNSGILYSPEEDINEREQIMYQAVHYEL
                     VASALAVQTGKSINPEFNIGCMIAMCPIYPLTCAPNDMMMATKAMHRRYWFTDVHARG
                     YYPQHMLNYFARKGFNLDITPEDNAILASGCVDFIGFSYYMSFTTQFSPDNPQLDYVE
                     PRDLVSNPYIDTSEWGWQIDPAGLRYSLNWFWDHFQLPLFIVENGFGAVDQRQADGTV
                     NDHYRIDYFASHIREMKKAVVEDGVDLIGYTPWGCIDLVSAGTGEMKKRYGMIYVDKD
                     NEGKGTLERIRKASFYWYRDLIANNGENI"
BASE COUNT         2467 a         2458 c         2162 g         2487 t
ORIGIN      
        1 gccggagcaa gacgtttagc gacaaaatcc gccgcatgtt gttcgatgac tggcgcgcct
       61 ttctccaggc aatactgacg ctctttcatg cccagacgaa agcgagagcg aaattttgaa
      121 cgggataaac gcgcgaagag tggatcgaga gactgcatta ataaaccgaa cgccctaact
      181 gtttcgtcaa ttgttcaagg acggcgatac ccgcaagcga gttacctgca tcatccagtt
      241 ccggactcca gactgcgatg gccatttcat gcggaacaat cgccacaata ccgccaccaa
      301 cgccagattt cgccggtagt cccacccgcc aggcaaactc ccccgcgttc tggtacatac
      361 cactggtcgc catcagcgcg ttaatttgcc gcgcctgcat tggcgtcact acaggttcat
      421 caatatgaat agctttcccc tgattagcca gaaagacaaa cgtccgagcc agctctacac
      481 agctcatttt cagagcgcag taatgaaagt agttttgcag aacggttgtc acgtcatgat
      541 ggaaattgcc aaacgacttc atcagccagg cgatagccgc atttcgcgcg gaatgttcaa
      601 attcggaacg cgctaccacc gtatcgtatg aaatatcaga cacaccgctt aagccgcgca
      661 cgacttccag catacgttgc cgtggtgcgc ttaatcgccc ttgcaacata tcgcagacca
      721 ccagcgcacc agcattaata aacggattac gcggaatacc ctgttccatt tccagttgca
      781 ctaaagaatt gaacggtgat ccagacggat ctttgccgac gcgttgccag atttcctctt
      841 cggagtaatg acgcatggcg acaacgagac tcagcacttt ggaaatagac tgaatggaaa
      901 aacgttcttg cgcgtctccg gcctgaaaaa gctgtccgtc aacggtacag atagcaatgc
      961 ccaatcggga accgtctact gtagccagcg ccggaatata atccgcgact ttaccctgac
     1021 caatgagcgg ccgcacttgc cgcaagatgt tttctaaaat tgcattatcc atggcgactg
     1081 ccactttcta ctcctggacc gcaggtctga aaagacctgc gagtatatca gagctgaata
     1141 tgtcgcgtca gatccggtct ttccacaccg tctggatatt acagaattcg tgtaagccga
     1201 aatgggaaag ctcacgacca aagccactct ttttcacgcc accaaaggcc actcgcgcgt
     1261 cgctggcaca ataaccattg ataaacaccc caccgcattc cagacgtgcc gccatctgtc
     1321 tggcctgtgt ttcgtcggtg gtaaaaatgg tcgctgaaag gccgaactca ctatcattag
     1381 ccagttccag cgcatgttct gcatctttcg caacggtgat tgccgcaacg gggccaaaca
     1441 tttcttcccg aaacgcggtc atttctgggg taacattcgc cagaaccgtt ggcggatagt
     1501 agttacctgc cccagccatc ttttccccgc ccagtaacaa acgcgcaccc tgcgccaggg
     1561 ttttctctac ctgatgatgc agctcatcac gtaaatcaaa acgagccatt ggtccgagag
     1621 tgttctcttc gtcacgggga tcgcccattt tcaaggctgc cgcagctgcc acaaaacgtt
     1681 cggtaaatgc agaagcaatt ccctcttcga taataaagcg ttttgccgct gcacatacct
     1741 gtccggtatt ctgataacgt ccggctaccg ccgctttaac cgccagttcc agatcggcat
     1801 cgttaagcac aataaacgga tccgaaccgc ccagttccag tacgcatttt ttcagtgccg
     1861 ctccagcctg tgcgccaata gccgctccag cacgaacact tccggtcacc gtgacagcag
     1921 caatgcgcga gtctttaatc atctgactga caccgtcgtt gtcggcattc agccagccat
     1981 atacgccttg cgggattccc gcatctttaa acacctgggc aatgagctga gcacatccca
     2041 tcacattcgg cgcatgttta agtaagtagc cgttacctgc aagaatgatg ggaacagcgc
     2101 cacgcatcac ctgccataac ggaaaattcc acggcataat cgccagaatc gtccccaacg
     2161 gtcgatactc aataaccgcc tgctgatttt ccaccagtgt aggttccgcc ttcagcattg
     2221 ccggaccatg ttctgcatac cagtcacaca aattcgccga tttcgccact tcagcgcgcg
     2281 cctggttgat tggtttgccc atttcgcggg tgatcatttg cgccatttct tcgctacggg
     2341 cgcgcagagc cttaccgata tcacgcattt tttcagcacg ataatctata tttgtctcgc
     2401 gccagtcgcg aaagcctgct gccgccagct caagtgcgtt ttcgatatcg ttagcgccag
     2461 cccacggcag cacagaaagt tgttcacccg tggcaggatt tattgaaatt gcatgagttg
     2521 ccggagtaat ggtcatcggg gtatctcctt tatgagtcat ggtatgaaga tacgcagatt
     2581 tactcttgct ttaaaatgaa taatattaag ccacttattc acgaatcgag aatgttatgg
     2641 atctgaccca actggagatg ttcaacgcgg ttgccgaagc tggcagcatt acccaggctg
     2701 cagcaaaagt gcatcgcgta ccgtcgaatc tcactacccg tctacgccag ctggaaacag
     2761 aactgggggt agatctgttt attcgcgaga atcagcgttt acgtctctct cctgccgggc
     2821 ataacttttt acgctatagc caacaaattc tcgcgttagt ggatgaagcg cggagcgttg
     2881 tcgctggcga tgagccgcaa ggtttatttt ctcttggttc gctggaaagc accgcagcag
     2941 tgcgcattcc ggccacgctg gcggaattca accatcgtta tcccaaaatt cagttttcac
     3001 tttccaccgg cccttccggc actatgctgg aaggagtgct ggagggaaaa ctgaatgcgg
     3061 catttattga tgggcccatt aaccatactg ccattgacgg gataccggta taccgcgagg
     3121 agctgatgat cgtcacgcca caaggtcatg cgccagtaat ccgcgccagt caggttaacg
     3181 gcagtaacat ttatgccttc cgcaccaatt gttcgtatcg tcgccacttc gagagctggt
     3241 ttcatgctga cggtgccgct ccgggaacta ttcacgagat ggagtcgtat cacgggatgt
     3301 tggcctgtgt ggtcgcagga gcaggcattg cgcttattcc acgctctatg ctggaaagta
     3361 tgccggggca tcaccaggtt gaagcgtggc cgttagctga gcaatggcgt tggttaacaa
     3421 cctggctggt ctggcgtcgc ggtgcgaaaa cgcgtccgct tgaggcattt attcaactac
     3481 tggatgtgtc tgactcggca aaacagagtt accaatgaac tattttgata attcttgcgg
     3541 ctaatatgct ctatatagtg atgttccgat gacttatgac tatatggggc aaatatggtt
     3601 acgccagtaa gcatcagcaa ttacatatct cttcctgatg attttcccgt tcgtaatatt
     3661 gcaccacagg taaaagaagt tttaaaggac tttattgatg cgctaagtac aataatatgt
     3721 gatgaagagt ggcgcacgtc tttaaacatc aactcagcca caaaaaagat atttaataac
     3781 cttgataact tatcatatat tcagaggact tctttccggg gtaacgacac gctatacaat
     3841 gaaaaggttc agtttaaact cacttatccc gtcaagaatg gaagacacaa agaaaatatt
     3901 gaatttcagg tagtaataaa tttaagccct atttatttag ataattttcg ccatgatgga
     3961 gaaattaata ttttttgcgc tcccaaccca aagcctgtca ctatggggcg cgtatttcag
     4021 accggcgtcg agcgtgtgct ctttctgttt ctgaatgatt ttattgaaca atttccaatg
     4081 ttcaaccctg gcgttccgat caaaatagcg catacaccac atattgaacc cctgcctccg
     4141 gatcaccata ccgctgcaga ttacttacgc cagtttgatt tgcttgtcct gaattttatc
     4201 tctcgcggta attttgtcat actcccccga ttatggaata actctgaggt tcacagatgg
     4261 ttcgtcaata aagatcctaa cttgatcacc gccattctcg acataacgga cagcgaatta
     4321 aaagaggatt tattgcaaag cctgatggat tcattaggtt ctaacaaaca tgtactaccc
     4381 gaagtctgca tctgcttttt attcctttta gcagaacaag aatctcctca ttttcaggac
     4441 ttgtttttat ttttcgccaa tatgttattg cactatcacc aatttatgaa tcccaatgaa
     4501 agtgatttga atgacgtgtt gatgccagca tcattaagtg atgataaaat tatcaagcat
     4561 atggcgcgca ggaccctcga actgtttgta aaaaatgaaa caccgccaaa agttactcac
     4621 gaagacctgg tgaaaaacag gcctcgctcc cctgtcagac cacctatacc cgcaacctcc
     4681 aaaacgccag acctccctga acgtcattaa accgtgatgt taccgactct ctgacgcgga
     4741 acagaatcag cgtcagagaa acggaaaacg cgatccagat cacaaataca ttgtattcac
     4801 atcattaacc gttttaagat catttcatca ctttttcgca actcacccga taatctgtta
     4861 tgacaacaaa cactgtttcc cgcaaagtgg cgtggctacg ggtcgttacg ctggcagtcg
     4921 ccgccttcat cttcaacacc accgaatttg tccctgttgg cctgctctct gacattgcgc
     4981 aaagttttca catgcaaacc gctcaggtcg gcatcatgtt gaccatttac gcatgggtag
     5041 tagcgctaat gtcattgcct tttatgttaa tgaccagtca ggttgaacgg cgcaaattac
     5101 tgatctgcct gtttgtggtg tttattgcca gccacgtact gtcgttttta tcgtggagct
     5161 ttaccgttct ggtgatcagt cgcattggtg tggcttttgc acatgcgatt ttctggtcga
     5221 ttacggcgtc tctggcgatc cgtatggctc cggccgggaa gcgggcacag gccttgagtt
     5281 taattgccac cggtacagca ctggcgatgg tcttaggttt acctctcggg cgcattgtgg
     5341 ggcagtattt cggttggcga atgaccttct tcgcgattgg tattggggcg cttatcaccc
     5401 ttttgtgcct gattaagtta cttcccttac tgcccagtga gcattccggt tcattgaaaa
     5461 gcctcccgct attgttccgc cgcccggcat tgatgagcat ttatttgtta actgtggtgg
     5521 ttgtcaccgc ccattacacg gcatacagct atatcgagcc ttttgtgcaa aacattgcgg
     5581 gattcagcgc caactttgcc acggcattac tgttattact cggtggtgcg ggcattattg
     5641 gcagcgtgat tttcggtaaa ctgggtaatc agtatgcgtc tgcgttggtg agtacggcga
     5701 ttgcgctgtt gctggtgtgt ctggcactgc tgctacctgc ggcgaacagt gaaatacacc
     5761 tcggggtgct gagtattttc tgggggatcg cgatgatgat catcgggctt ggtatgcagg
     5821 ttaaagtgct ggcgctggca ccagatgcta ccgacgtcgc gatggcgcta ttctccggca
     5881 tatttaatat tggaatcggg gcgggtgcgt tggtaggtaa tcaggtgagt ttgcactggt
     5941 caatgtcgat gattggttat gtgggcgcgg tgcctgcttt tgccgcgtta atatggtcaa
     6001 tcattatatt tcgccgctgg ccagtgacac tcgaagaaca gacgcaatag ttgaaaggcc
     6061 cattcgggcc ttttttaatg gtacgtttta atgatttcca ggatgccgtt aataataaac
     6121 tgcacaccca tacataccag caggaatccc atcagacggg agatcgcttc aatgccaccc
     6181 ttgcccacca gccgcataat tgcgccggag ctgcgtaggc ttccccacaa aataaccgcc
     6241 accaggaaaa agatcagcgg cggcgcaacc atcagtaccc aatcagcgaa ggttgaactc
     6301 tgacgcactg tggacgccga gctaataatc atcgctatgg ttcccggacc agcggtactt
     6361 ggcattgcta gcggcacaaa ggcaatattg gcactgggtt catcttccag ctcttccgac
     6421 ttgcttttcg cctccggtga atcaatcgct ttctgttgcg gaaagagcat ccgaaaaccg
     6481 ataaacgcga cgattaagcc gcctgcaatt cgcagaccgg gaatcgaaat gccaaatgta
     6541 tccatcacca gttgcccggc gtaatacgcc accatcatga tggcaaatac gtacaccgag
     6601 gccatcaacg actgacgatt acgttcggca ctgttcatgt tgcctgccag gccaagaaat
     6661 agcgcgacag ttgttaatgg gttagctaac ggcagcaaca ccaccagccc caggccaatt
     6721 gctttaaaca aatctaacat tggtggttgt tatcctgtgt atctgggtta tcagcgaaaa
     6781 gtataagggg taaacaagga taaagtgtca ctctttagct aaccttgcat cgcattgacc
     6841 aaaacttgaa ccgatttagc aaaacgtggc atcggtcaat tcattcattt gacttatact
     6901 tgcctgggca atattatcct ctgcaactaa ttacttgcca gggcaactaa tgtgaaaagt
     6961 accagcgatc tgttcaatga aattattcca ttgggtcgct taatccatat ggttaatcag
     7021 aagaaagatc gtctgcttaa cgagtatctg tctccgctgg atattaccgc ggcacagttt
     7081 aaggtgctct gctctatccg ctgcgcggcg tgtattactc cggttgaact gaaaaaagtg
     7141 ttgtcggtcg acctgggagc actgacccgt atgctggatc gcctggtctg taaaggctgg
     7201 gttgaaaggt tgccgaaccc gaatgataag cgcggcgtac tggtaaaact taccaccagc
     7261 ggcgcggcaa tatgcgaaca atgccatcaa ttagttggcc aggacctgca tcaagaatta
     7321 acaaaaaacc tgacggcgga cgaagttgca acacttgagc atttgcttaa gaaagtcctg
     7381 ccgtaaacaa aaaagaggta tgacgatgtc cagacgcaat actgacgcta ttaccattca
     7441 tagcattttg gactggatcg aggacaacct ggaatcgcca ctgtcactgg agaaagtgtc
     7501 agagcgttcg ggttactcca aatggcacct gcaacggatg tttaaaaaag aaaccggtca
     7561 ttcattaggc caatacatcc gcagccgtaa gatgacggaa atcgcgcaaa agctgaagga
     7621 aagtaacgag ccgatactct atctggcaga acgatatggc ttcgagtcgc aacaaactct
     7681 gacccgaacc ttcaaaaatt actttgatgt tccgccgcat aaataccgga tgaccaatat
     7741 gcagggcgaa tcgcgctttt tacatccatt aaatcattac aatagctagt tgataacgtg
     7801 acaacgtcac tgaggcaatc atgaaaccac tttcatccgc aatagcagct gcgcttattc
     7861 tcttttccgc gcagggcgtt gcggaacaaa ccacgcagcc agttgttact tcttgtgcca
     7921 atgtcgtggt tgttccccca tcgcaggaac aaccaccgtt tgatttaaat cacatgggta
     7981 ctggcagtga taagtcggat gcgctcggcg tgccctatta taatcaacac gctatgtagt
     8041 tattgtcagg ctctgatcac agagcctgac atctccgtaa tatcttcctc aagtctcaaa
     8101 tattttcgcc attgttggcg atgagatccc gataccagta aaacgacgct ttacgtatcc
     8161 gttccagcgt tcccttccct tcgttgtctt tatcgacata aatcattccg tagcgctttt
     8221 tcatttcccc tgttccggca gaaaccaggt caatgcagcc ccacggggtg tagccaatta
     8281 agtcaacacc atcttcaact acggcttttt tcatttcccg aatatgggaa gcaaagtaat
     8341 caatgcgata gtgatcgttc accgtgccgt cagcttgtct ctggtcaacc gcaccaaatc
     8401 cattttcgac aataaacagc ggcaactgga aatgatccca gaaccagttg agtgaataac
     8461 gtagccctgc cggatcaatt tgccatcccc attcggatgt atcgatataa gggttgctga
     8521 ccaaatctcg tggttcaaca taatccagtt gcgggttatc gggagaaaat tgtgtcgtaa
     8581 aagacatgta gtagctaaaa ccgataaagt cgacacatcc actggcaaga atcgcgttat
     8641 cttctggtgt gatatcgagg ttgaatcctt tcctggcaaa gtaattcagc atatgttgcg
     8701 gataatatcc acgagcatga acatcagtaa accagtaacg acgatgcatc gctttcgtgg
     8761 ccatcatcat atcgttgggt gcacacgtca gaggatagat ggggcacatg gcgatcatac
     8821 agccaatgtt aaattcagga ttgatcgatt ttccagtctg taccgccagg gcactggcaa
     8881 ctaactcgta atgtaccgcc tggtacatta tttgttcgcg ctcattgata tcttcctctg
     8941 gcgaatacaa gataccggaa ttagtaaatg gacacaggct ttcgctgaaa ttcacctgat
     9001 tattgatttc gttaaacgtc atccagtact ttactttttc tttatagcgc gtgaagaccg
     9061 ttgatgcgaa gcggataaaa aagtcgatca gcttacggtt tcgccagcca ccatattttg
     9121 tcaccagatg ataaggcatc tcaaaatgcg aaagcgtcac cacaggttcc attccttgct
     9181 tcaggcattc atcgaacaga tcatcataaa attgtaaacc ctcttcattc ggctcctgtt
     9241 cgtcaccttg cggaaagatt cgtgtccagg caatggaagt tcgaaagcat ttgaatccca
     9301 tctcggcaaa taactgaata tctgttttat agcgatgata aaaatcaatt gcttcatgat
     9361 tgggataatt aagcccgtcg ataacgcctt ctgtcacttc acgcggcacc ccgtgagcgc
     9421 cagcagtcat cacatcagcg atactgatgc cttttcctcc ttcattccag ccaccttcca
     9481 actgatgcgc ggctaccgcg ccaccccata aaaaaccttt tttaaatcct gacatacaca
     9541 actccttaac taaaatagat taccaccaga cttc
//
LOCUS       DAJTZL010000152         9432 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.152, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000152 DAJTZL010000000
VERSION     DAJTZL010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9432
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.152"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            205..1071
                     /gene="folD"
                     /locus_tag="OO544_003270"
     CDS             205..1071
                     /gene="folD"
                     /locus_tag="OO544_003270"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HCR6768794.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            1073..1285
                     /gene="ybcJ"
                     /locus_tag="OO544_003271"
     CDS             1073..1285
                     /gene="ybcJ"
                     /locus_tag="OO544_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HCR6768795.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKADGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            1393..1914
                     /locus_tag="OO544_003272"
                     /pseudo
     CDS             1393..1914
                     /locus_tag="OO544_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415060.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
     gene            complement(1950..3335)
                     /gene="cysS"
                     /locus_tag="OO544_003273"
     CDS             complement(1950..3335)
                     /gene="cysS"
                     /locus_tag="OO544_003273"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HCR6768796.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYAADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTEKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            3509..4003
                     /gene="ppiB"
                     /locus_tag="OO544_003274"
     CDS             3509..4003
                     /gene="ppiB"
                     /locus_tag="OO544_003274"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HCR6768797.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVEGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            4006..4728
                     /gene="lpxH"
                     /locus_tag="OO544_003275"
     CDS             4006..4728
                     /gene="lpxH"
                     /locus_tag="OO544_003275"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HCR6768798.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDEPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            4846..5355
                     /gene="purE"
                     /locus_tag="OO544_003276"
     CDS             4846..5355
                     /gene="purE"
                     /locus_tag="OO544_003276"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HCR6768799.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVLHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGTA"
     gene            5352..6419
                     /gene="purK"
                     /locus_tag="OO544_003277"
     CDS             5352..6419
                     /gene="purK"
                     /locus_tag="OO544_003277"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HCR6768800.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWRLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYTLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFS"
     gene            complement(6556..7449)
                     /locus_tag="OO544_003278"
     CDS             complement(6556..7449)
                     /locus_tag="OO544_003278"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCR6768801.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRGKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(7446..8099)
                     /locus_tag="OO544_003279"
     CDS             complement(7446..8099)
                     /locus_tag="OO544_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HCR6768802.1"
                     /translation="MTIIHPLLASRSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     AHPQPLAAAWMQRAEETGLFGPLALAAGDPLPAELRQFFAYSDNLQLVTTAVSVSYLR
                     YAAQGYFASPLLHFVHALSCPKRTAVAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            complement(8110..9369)
                     /locus_tag="OO544_003280"
     CDS             complement(8110..9369)
                     /locus_tag="OO544_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HCR6768803.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELNEGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAILEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRALAPQIARLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIYLERNMQLQIPGWDFQGACLGLDIRRVVETDITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
BASE COUNT         2102 a         2673 c         2498 g         2159 t
ORIGIN      
        1 tagacaagga aataagtcag ttccagattt acaacgccat catggacgaa aaatgaagct
       61 ttcaatctca gcgacggtgc gccccacctt cgcaagaggt cgcttcacgc gataaatctg
      121 aaacgaaacc tgacagcgcg ccccgcttct gacaaaatag gcgcatcccc ttcgacctac
      181 gtaacagatg gaatcctctc tctgatggca gcaaagatta ttgacggtaa aacgattgcg
      241 cagcaggtgc gctctgaagt tgctcaaaaa gttcaggcgc gtattgcagc cggactgcgg
      301 gcaccaggac tggccgttgt gctggtgggt agtaaccctg catcgcaaat ttatgtcgca
      361 agcaaacgca aggcttgtga agaagtcggg ttcgtctccc gctcttatga cctcccggaa
      421 accaccagcg aagcggagct gttggagctt atcgatgcgc tgaatgccga caacaccatc
      481 gatggcattc tggttcaact gccgttaccg gcgggtattg ataacgtcaa agtgctggaa
      541 cgtattcacc cggacaaaga cgtagacggt ttccatcctt ataacgtcgg tcgcctgtgc
      601 cagcgcgcgc cgcgtctgcg cccctgcacc cctcgcggca tcgtcacgct gcttgagcgt
      661 tacaacattg ataccttcgg cctcaacgcc gtggtgattg gcgcatcgaa tatcgtcggt
      721 cgtccaatga gcatggaact gttgctcgca ggttgcacca ctaccgtgac tcaccgcttc
      781 actaaaaatc tgcgtcacca cgtagaaaat gccgatctac tgatcgttgc cgtcggcaag
      841 ccgggcttta ttcctggtga ctggatcaaa gaaggcgcaa ttgtgattga tgtcggcatc
      901 aaccgtctgg aaaatggcaa agttgtgggc gacgtcgtgt ttgaagacgc ggctaaacgc
      961 gcctcataca ttacgcctgt tccgggcggc gttggcccga tgacggttgc cacgctgatt
     1021 gaaaacacgc tacaggcgtg cgttgaatat catgatccac agggtgagta acatggcgac
     1081 attttcttta ggtaaacatc cgcacgttga gctgtgcgac ttgctgaaac tggaaggctg
     1141 gagcgaaagc ggcgcgcagg cgaaaatcgc gattgccgaa ggccaggtga aagccgacgg
     1201 cgcggttgaa acgcgcaaac gctgcaaaat cgtcgccggt cagacagtga gttttgcagg
     1261 tcacagcgta caggttgttg cctgatccct ttcccggcct gtatcaacag gccggttctc
     1321 ttttcagact tttgccctct acgccccgtt catccatgct gatagcatcg acattttgcc
     1381 gaagcgacca tcatgccaac cgttattacg cacgccgccg ttcccctatg cattggttta
     1441 gggttgagtt cgaaagtcat ccctccgcgt ctgttatttg cctgaatcat cctggcgatg
     1501 ctgcccgacg ccgacgtatt gtcgtttaaa tttggcgttg cttacggcaa tgtttttggt
     1561 catcgcgggt ttacccattc gctggtgttt gcgtttgttg tcccgctgtt atgtgtgctg
     1621 attggacgcc gatggttcag ggctgggctg attcgctgct ggctgttttt aaccgtctcg
     1681 ttgctatcgc acagcatgct ggattcggta accactggcg gtaagggtgt tggctggctg
     1741 tggccgtggt cagatgaacg ctttttcgct ccctggcagg tgattaaagt cgcgccgttt
     1801 gcattgtctt gttacaccac gccgtacggg catcaggtga ttatttctga attgatgtgg
     1861 gtgtggttgc cggggatgct actgatggga atgttgtggt ggcgcagacg ataaccggat
     1921 gcgaaaactc gcatccggca atagcgcaat tacttacgac gccaggtggt cccttgcggg
     1981 ccatcttcca gaacgatccc catctcgtta agacggtcac gcgccgcatc tgccgccgcc
     2041 cagtctttcg ctttacgggc atccagacgc tgttgaatta acgcttcaat ctcagccact
     2101 tcgctgtcgt ctgcctgcgc gccgctttgc aggaacgctt ccggttcttg ctccagcagg
     2161 cctaatacag cggaaagttt acgcaggtga gatgccattg cattcgccgc tgccatatct
     2221 tctgctttca gacggtttac ttcacgcgcc atatcaaaca gtacggaata ggcttccggg
     2281 gtgttgaaat cgtcgtccat cgcttcaata aagcgcgctt caaacgcttc gccaccggca
     2341 ggagcaacgg ttttttctgt gccgcgcagg gcagtgtaga gacgctccag cgccgcacgc
     2401 gcctgcttca ggttctcttc gctgtagttc agctggctgc gatagtggcc cgacatcagg
     2461 aagtaacgca cggtttcagc atcgtagtat ttcagcacat cgcgcacggt aaagaagtta
     2521 cccagcgatt tggacatctt ctcgcggtca accatcacca ttccggagtg catccagtag
     2581 ttaacatact gaccatcatg ggcacaggtg gactgcgcga tttcattctc gtggtgcggg
     2641 aacatcaggt cagaaccgcc gccgtggata tcaaagtggt tacccagctg cttgcagttc
     2701 attgccgaac attcaatgtg ccagcctgga cggcccgcgc cccacggaga cggccagctc
     2761 ggttcgccct ctttcgacat cttccacaga acgaagtcca tcgggttgcg tttgtcgtcg
     2821 accacgtcaa cgcgcgcgcc tgcctgcagc tggtcgagat cctgacgcga cagcacacca
     2881 taagtcggat cggtcgggac gtcgaacatc acgtcgccgt tgtccgccgc ataagcgtga
     2941 cctttggcga tcagttgttc agtgagttca ataatttctg cgatatggtg cgtcgcgcgc
     3001 ggctccatat ccgggcgcag aatgttcaaa gcatcaaaat ctttgtgcat ttcggcgatc
     3061 atgcgatcca ccagcgccac aaagctttcg ccattttcat tggcacgttt gatgattttg
     3121 tcgtcgatat cggtaatgtt gcgcacatac ttcagtttat agccgaggaa acgcagatag
     3181 cgcgcaacca cgtcaaaagc aacaaaggta cgcccgtgac caatatgaca gagatcgtaa
     3241 acggtgattc cacacacata catgccgact tccccggcgt gaataggctt aaattcctct
     3301 ttttggcgtg tcagagtatt gaagattttt agcatcgaag attccgttta gacatgtgtg
     3361 ggtaattgag ttgcgtataa tacccatatt tcccgcgcga atcagcatac attgcgtgat
     3421 gatccgatcg tgcggttatg ctataacacc accctatata tgacccgaac tgggttgaag
     3481 caccaatcaa acggaacagg atgcaaaaat ggttactttc cacaccaatc acggcgatat
     3541 tgtcatcaaa acttttgacg ataaagcacc tgaaacagtt aaaaacttcc tggactactg
     3601 ccgcgaaggt ttttacaaca acaccatttt ccaccgtgtt atcaacggct ttatgattca
     3661 gggcggcggt tttgaaccgg gcatgaaaca aaaagccacc aaagaaccga tcaaaaacga
     3721 agccaacaac ggtctgaaaa atacccgtgg tacgctggca atggcccgta ctcaggctcc
     3781 gcactctgca actgcacagt tcttcatcaa cgtggttgat aacgacttcc tgaacttctc
     3841 tggcgaaagc ctgcaaggtt ggggctactg cgtgtttgct gaagtggttg aaggcatgga
     3901 cgtggtagac aaaatcaaag gtgttgcaac cggtcgtagc ggtatgcacc aggacgtgcc
     3961 aaaagaagac gttatcattg aaagcgtgac cgttagcgag taatcgtggc gacactcttt
     4021 attgcagatc ttcatctctg cgtggaagaa ccggcgatca ccgccggttt tctgcgtttt
     4081 ttagcggggg aagctcgcaa ggccgacgcg ctgtatattc ttggcgatct gtttgaagcg
     4141 tggattggcg acgacgagcc caatccactc catcgccaga tggcggcagc gatcaaagcg
     4201 gtgtccgatt ctggcgttcc ctgttatttc attcatggca accgtgattt tctgctcggc
     4261 aaacgctttg cccgtgaaag tggcatgacg ttattgccgg aagaaaaggt gctcgaactt
     4321 tatggccgcc gggtgttgat tatgcatggc gacacgctgt gcaccgatga cgcgggttat
     4381 caggcttttc gcgccaaagt ccacaaaccc tggctgcaaa tgctattcct cgccctgccg
     4441 ttgtttgtgc gcaaacgcat tgccgcgcga atgcgagcga acagtaaaga agccaacagc
     4501 agtaaatcgc tggcgatcat ggacgttaac caaaacgcgg tagtcagtgc gatggaaaaa
     4561 catcaggtgc aatggctgat ccacggacat acccatcgcc cggcggtaca tgaacttatc
     4621 gccaaccagc aacctgcttt tcgcgtggta ctcggtgcct ggcatacgga aggttcgatg
     4681 gtgaaagtca cggcggatga cgttgagctg attcattttc cgttttaaaa aacccgcaac
     4741 tttgctgatt tcacagccac gcaaccgttt tccttgctct ctttccgtgc tattctctgt
     4801 gccctctaaa gccgagagtt gtgcaccaca ggagttttaa gacgcatgtc ttcccgcaat
     4861 aatccggcgc gtgtcgccat cgtgatgggg tccaaaagcg actgggctac catgcagttc
     4921 gccgccgaaa tcttcgaaat cctgaatgtc ctgcaccacg ttgaagtggt ttctgctcac
     4981 cgcacccccg ataaactgtt cagcttcgcc gaaagcgccg aagagaacgg ttatcaggtg
     5041 attattgcgg gcgcaggtgg cgcagcgcac ctgccaggca tgattgccgc caaaacgctg
     5101 gtgccggtgc tgggcgtgcc agtacagagc gccgcactga gcggtgtcga tagcctctac
     5161 tccatcgtac aaatgccgcg cggcattccg gtgggtacgc tggcgattgg taaagctggc
     5221 gcggcaaacg cggcattact ggcagcacaa attctcgcga ctcacgataa agagctacac
     5281 cagcgtctga atgactggcg caaagcacaa actgatgaag tgctggaaaa cccggacccg
     5341 cgaggtacgg catgaaacag gtttgcgtcc tcggtaacgg gcagttaggc cgtatgctgc
     5401 gtcaggcagg cgaaccgtta ggcattgctg tctggccagt cgggctggac gctgaaccgg
     5461 cggcggtgcc ttttcaacaa agcgtgatta ccgctgagat cgaacgctgg ccggaaaccg
     5521 cattaacccg cgagctggcg cgccatccgg cctttgtgaa ccgcgatgtg ttcccgatta
     5581 ttgctgaccg tctgactcag aagcagcttt tcgataagct ccacctgccg accgcgccgt
     5641 ggcagttact tgccgatcgc agcgagtggc ctgcggtgtt tgatcgttta ggtgaactgg
     5701 cgattgttaa gcgtcgcact ggtggctatg acggtcgcgg tcaatggcgt ttacgcgcca
     5761 atgaaaccga acagttaccg gcagagtgtt acggcgaatg tattgtcgag cagggcatta
     5821 acttctctgg tgaagtgtcg ctggttggcg cgcgcggctt tgatggcagc accgtgtttt
     5881 atacgctgac gcataacctg catcaggacg gtattttgcg caccagcgtc gcttttccgc
     5941 aggccaacgc acagcagcag gcgcaagccg aagagatgct gtcggcgatt atgcaggagc
     6001 tgggctatgt gggcgtgatg gcgatggagt gttttgtcac cccgcaaggt ctgctgatca
     6061 acgaactggc tccgcgtgtg cataacagcg gtcactggac acaaaatggt gccagcatca
     6121 gccagtttga gctgcatctg cgggcgatta ccgatctgcc gttaccgcaa ccagtagtga
     6181 ataatccgtc ggtgatgatc aatctgattg gtagcgatgt gaattatgac tggctgaaat
     6241 tgccgctggt gcatctgcac tggtacgaca aagaagtccg tccggggcgt aaagtggggc
     6301 atctgaattt gaccgacagc gacacatcgc gtctgactgc gacgctggaa gccttaatcc
     6361 cgctgctgcc gccagagtat gccagcggcg tgatttgggc gcagagtaag ttcagttaag
     6421 caggtgctca tttgattgcc ggatgcggcg taaacgcctt atccggctta ccgctccggt
     6481 acccattgta ggcctgataa gacgcgtcaa gcgtcgcatc aggcatggtg caccgattgc
     6541 cggatgctct catcactaca gcgaaataca ggtccccgct tcgcccgcca gcgtctcttc
     6601 aattcgcgat aacgccccaa tccacgcggg tttaccacgg cttctgacat aaccactcac
     6661 cgccgttacc ttcggcccca tcgaaccatc ggctttggca aatggcgcta actcatccgg
     6721 tgtggcatgg cgaatggcac gttgctgcgg cgttccccag ttttcatata ccgcatcagc
     6781 atcggtgagg atcaccagtc catcggcatt aatctgctcg gcgagcaacg cagcggcgag
     6841 atctttatca atcactgctt cactccctgc cccatcttcc gtcacaggca cacctccgcc
     6901 gccactgcaa atcaccacat gcccctcttt gagcaacaac tcgatggctt cgctgtcgag
     6961 aattttacgc ggttgcggag acgccaccac ccggcgcaaa tatttaccat cacgtttcat
     7021 ctgccagccg taagccgctt ccagtgcctc ttgttcttct ggctgataaa ccggaccaat
     7081 aaatttctct ggctgcaaaa acgccggatc atcaggcgaa acctcaatgc gcgtcagcac
     7141 cgtcgtaaca ggcggcatct gcggctgtgc gctcaagctc tgcgccagca tatagccaat
     7201 catcccctgg ctttccgcca ccagcacatc cagcggatac ggttctacct ctttccacgc
     7261 cagattctgt aacgccagca gccccacctg cggcccgttg ccgtgaacaa tcgccagccg
     7321 ataagaacgg gccaggcgtg ccagcgcggg tacagcactg gcgatattgc gatattgatt
     7381 ttccgccgtc agcgcctcac cgcgctggag taaggcgttg cccccaagag ctacaaccag
     7441 tgttttcatg gttttccttg taataattgt tggccaagcc agaaccccag cagcgtatct
     7501 gcccctgacg tatgccccag cgccagcagc gaatcaatcg caacagcggt acgtttcggg
     7561 caactcagag catggacaaa gtgcaggagt ggcgaggcga aatatccttg cgcggcataa
     7621 cgtaaataac tgacgctcac cgctgtggta acgagttgaa gattgtcgga ataggcaaaa
     7681 aactggcgta actcagcagg cagcggatcg cctgccgcca acgccagtgg cccgaaaagc
     7741 ccggtttcct ccgcgcgttg catccatgca gctgcaagcg gttgaggatg cgcaggcggc
     7801 gtaatacgca gcaaacaata acgctgtggc tgtttaaccg tgaaacgccc gaggcgaatc
     7861 ccttgagcca caacctgtgg tcgttcattg ccgcataatc caccgcataa cgcatcgaat
     7921 tgcgcgcggc gaagcaccca tcctccgggg ccgaaaccac taccctgacg atgcagcgtt
     7981 aacagttcgc cgctttccgt catcaggtta atcgcccgtc gccacactcc cgctaaccgc
     8041 caggactggc gataattcgg tgcactacgg ctggcaagca gaggatggat gatcgtcatt
     8101 gcgcgttcct caaccaatcc ccatgctttc agccagtgct tccagcgcct gttcaaaaca
     8161 cgccagcggt gcccgcacag tgcctgcgcc aatctgcccg atccccgcct ctttatgggc
     8221 gataccggta ttgatgagtg gcgtaatgtc ggtttctacc acgcgacgaa tgtccagtcc
     8281 caggcacgcg ccctgaaaat cccagcctgg gatctgcaac tgcatattgc gctcaaggta
     8341 aatttccgcc atctcttcag ataccgctct ggccgcttcc atgccacccg caccgacaaa
     8401 gcgcgttacg ccaggcgctg cgatcattgc cgcacctccg ataccaaagg tttcggtaat
     8461 cgcgctatcg cccatatccg ggttcgcctg ctcctgcgag aagccggtga aaaacagacc
     8521 ttgcggagtg tttacaggcg cagtaaacca gcgttcgccc agcccgctta cccgaatccc
     8581 gaacatattg ccgttgcggg tcattgccgt gacgatgctg cctgcgcgga tcatcgcgcc
     8641 agcatccatc gccgccttgc agtaagccat cgcgaggttg aggaagaact gatcggtcac
     8701 gctgaggaaa tccatcactt cggcgatgtg ctgtttatca tgatcgaggc gagcaatttg
     8761 tggggccagc gcacgcatta acagtgcaga ggaagcaata ttgcgttgat ggaactcatc
     8821 gcccatcgta atgccctgcg ccatcatcgc cgtgagatcg ataccgcgct ccatgcgccc
     8881 cagcgccgcg cttaatactg gcattaacac atcgcgcatc cagcgatggc gagtcaggac
     8941 atcttcgccg taagcgccaa aacgcatcac tttgccgata ccttcgttga ggttgcagta
     9001 cgcccggttg ccgtcggtca cgttctcgac caccagcatc ggcatactgg cagaagtaat
     9061 accgcccatt ggaccgacgg cattcacatg gtgacaagga atgaagttca cttccccctg
     9121 ctccagtatt gccagcgcct gcgcttcatc tttcgcccag ccttcgaaca gacatgcgcc
     9181 cacgcacgcc cctttcatgg gtccggtcat ctcctgccag cgcattggcg gcccggcgtg
     9241 aagcagtgtt ttgccctcgt ttagttcgct gataagtgaa gaagccggtt gcacatccag
     9301 ccagtgtgga cgagcgcgac gaatttgttc gataaccgca gcattggctt gcgccactga
     9361 tgtaaacatg ggaacccctt attgcaaacg ttctaataaa cgagccaggt aatgactcca
     9421 acttattgat ag
//
LOCUS       DAJTZL010000153         9397 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.153, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000153 DAJTZL010000000
VERSION     DAJTZL010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9397
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.153"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(350..1603)
                     /locus_tag="OO544_003281"
     CDS             complement(350..1603)
                     /locus_tag="OO544_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6768804.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKTLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHRRLHA"
     gene            1710..2603
                     /locus_tag="OO544_003282"
     CDS             1710..2603
                     /locus_tag="OO544_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6768805.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPNVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPASRNFRIHLLNALR"
     gene            2738..3958
                     /gene="mlc"
                     /locus_tag="OO544_003283"
                     /gene_synonym="dgsA"
     CDS             2738..3958
                     /gene="mlc"
                     /locus_tag="OO544_003283"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor Mlc"
                     /protein_id="HCR6768806.1"
                     /translation="MVAENQLGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            4083..4778
                     /gene="bioD"
                     /locus_tag="OO544_003284"
     CDS             4083..4778
                     /gene="bioD"
                     /locus_tag="OO544_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HCR6768807.1"
                     /translation="MLERFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEVIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(4731..6023)
                     /gene="clcB"
                     /locus_tag="OO544_003285"
     CDS             complement(4731..6023)
                     /gene="clcB"
                     /locus_tag="OO544_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HCR6768808.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAAFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALSASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYSVQLSVTVQASDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(6182..6796)
                     /gene="dmsD"
                     /locus_tag="OO544_003286"
     CDS             complement(6182..6796)
                     /gene="dmsD"
                     /locus_tag="OO544_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HCR6768809.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(6839..7693)
                     /locus_tag="OO544_003287"
     CDS             complement(6839..7693)
                     /locus_tag="OO544_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HCR6768810.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQRIV
                     RGMFFLWLLMGIGFMASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAGVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLVLGLILILVGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(7695..8312)
                     /gene="dmsB"
                     /locus_tag="OO544_003288"
     CDS             complement(7695..8312)
                     /gene="dmsB"
                     /locus_tag="OO544_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HCR6768811.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTSDTTGYLANPEEV"
     gene            complement(8323..>9397)
                     /locus_tag="OO544_003289"
     CDS             complement(8323..>9397)
                     /locus_tag="OO544_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HCR6768812.1"
                     /translation="RDGVRGKEKLDVPIKFLWCYASNTLINQHGDINHTHEVLQDDSK
                     CEMIVGIDHFMTASAKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQPATSAKFER
                     KPIYWMLSEVAKRLGPDVYQTFTEGRSQHEWIKYLHAKTKERNPEMPDYEEMKTTGIF
                     KKKCPEEHYVAFRAFREDPQANPLKTPSGKIEIYSERLAKIADTWELKKDEVIHPLPA
                     YTPGFDGWDDPLRKTYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDAQARG
                     IRHGDTVRVFNNNGEMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSINILT
                     SHRPSPLAKGNPSHSNLVQIEKV"
BASE COUNT         2449 a         2378 c         2439 g         2131 t
ORIGIN      
        1 cagacttccg tgagtggttg gtttcagcgt gtaacgatat gtacaaacgc tgaataaatt
       61 acagcattga taataacgta ttgtgactta agggaaattt agctaccaat aatagtagtc
      121 ttgatcgggt taactatttt actacttaca aagcggtaga ataactgcgc atcagtaata
      181 aatgacacac agcaaaatga atccgtttat ttgggtactt ataatcctga tgacgctaga
      241 cgcgctgcgg gaattggctg gcgcttcgtc tattttagga tggctattaa cgttggtttg
      301 agcaagcaat aagtccggac gtgtagttac cgcagtccgg gcttattttt caggcgtgca
      361 gacgacgatg caaacgcgtc ccgaccagca gagcaatgac cagcatcaga gcaataaatg
      421 cgccgacgcc gttccagcca tagttatgcc agaaaacacc acccagcgtc ccggcaatac
      481 tcgaccccag atagtaactg aacaggtaca gcgaggaggc ctggccttta gcgcgttttg
      541 cgcgggggcc aatccagctg ctggcaactg agtgggctgc gaagaatcct gctgagaaga
      601 gtaacattcc ggcaaagatc agccacagcg agctgaataa ggtcatcagt aaaccaaaca
      661 gcataacccc cgtcgaaaac aacatcaccg gaccgcgccc gtagcgggtg gtcatggttc
      721 cggctttggg tgagctccat gtaccggtca aataagccag cgataataag ccaaccacgg
      781 cctgactgac atgccagggg gaaagcatca accgatagcc gatgtaatta aacagcgtga
      841 cgaacgaccc catcagcaaa aagccttctg cgaacaataa cggtaatccc cggtcacgcc
      901 agtgcagacg aaagttgata aataacgtct tagggcgcag cgaagtcggg cgaaaatggc
      961 gtgattccgg aaggattttc cagaacatca acgccgaggc tagcgcgaaa caaccgattg
     1021 ccgccagagc aattcgccag ttgaaaaagt ccgtgaagac accactaatt aagcgtccgc
     1081 tcatgccgcc aattgagttg ccgctgatat acaaccccat cgaaaaggcc acgaaactgg
     1141 ggtggatttc ctcgctaaga taagtcatgc caacagctgc cacgccactt aacgaaagcc
     1201 caatcaaggc gcgcataatc aaaatgccgt gccagctggt catcattgtc gaaagtaacg
     1261 tacaaatgga ggccaacagt agcgccgtga ccatcactgg tttgcgacca atggcatcgg
     1321 atagggggcc agtaaacagc aaaccaatag ccaacatcgc cgtggaaatg gacagtgaaa
     1381 tactactgtt cgcgggggtt aagccaaact cctgcgaaag caccggaagg ataggctgca
     1441 cacaatagag gagtgcaaag gttgccagtc cggcagagaa cagcgccagg gtgacgcgca
     1501 taaattgcgg cgtaccgcgt ttaataaatt gatttggctg agaaatgctt tgcttgtcag
     1561 tgtcgcttgc cggagcgcca tcaacagttg tagtacggct cacttgaaat ccttgctaaa
     1621 tatgcctgtg gatcaggctt atacataggt taggaaaatc gaattgttct gtctaatata
     1681 ttaataatct caaataagat gttttaaata tgaatattga acttcgtcat ctgcgttact
     1741 ttgttgctgt tgcggaagag ctgcatttcg ggcgcgccgc tgcccgcctg aatatttcgc
     1801 aaccaccgct aagtcagcag attcaggcac tggagcaaca aattggtgcc cgactgctgg
     1861 cacgaactaa tcgcagtgta ttgctgacgg cagcaggaaa acagtttctt gcagatagtc
     1921 ggcaaatcct gtctatggta gatgacgctg ccgctcgcgc tgaaagactg catcagggtg
     1981 aagcggggga gttgcgcatt ggttttactt cgtccgctcc ttttattcgg gcggtgtccg
     2041 atacgttatc gctgtttcgc cgtgattatc ccgatgtcca tttacaaacc cgcgaaatga
     2101 acactcgtga gcaaatcgct ccgctcattg aaggaacgct ggatatggga ttgctgcgta
     2161 acacagcgtt accggagacg cttgaacacg cagtcatcgt ccatgaaccg cttatggcga
     2221 tgatcccgca cgatcatccc ctggcaaata acccgaatgt aacgctggct gaactggcga
     2281 aagaaccctt cgtctttttt gatccgcacg tcgggacagg gctgtatgac gatattctcg
     2341 ggctgatgcg acgttaccat ttgacgcccg tcatcactca ggaggtgggc gaggcaatga
     2401 ccatcatcgg tctggtttcc gccggtctgg gtgtttcaat tttgcctgct tcatttaaac
     2461 gtgttcagct caacgaaatg cgctgggtgc cgattgctga agaggatgcg gtttctgaaa
     2521 tgtggttggt ctggccgaaa catcatgaac aaagtccagc ttcgcgtaac tttcgtattc
     2581 atctgctgaa tgctctcagg tgagggaaat ttcagcgaaa aagcccgaaa aatgtgctgt
     2641 taatcacatg cctaagtaaa aatttgacga cacgtattga agtgcttcac catagcctac
     2701 agattatttc ggagcgcgaa aatataggga gtatgcggtg gttgctgaaa accagcttgg
     2761 gcacattgat caaataaagc agaccaacgc gggcgcggtt tatcgcctga ttgatcagct
     2821 tggtccagtc tcgcgtatcg atctttcccg tctggcgcag ctggctcctg ccagtatcac
     2881 taaaattgtc cgtgagatgc tcgaagcaca cctggtgcaa gagctggaaa tcaaagaagc
     2941 ggggaaccgt ggccgtccgg cggtggggct ggtggttgaa actgaagcct ggcactatct
     3001 ttctctgcgc attagtcgcg gggagatttt ccttgctctg cgcgatctga gcagcaaact
     3061 ggtggtggaa gagtcgcagg aactggcgtt aaaagatgac ttgccattgc tggatcgtat
     3121 catttcccat atcgatcagt tttttatccg ccaccagaaa aaacttgagc gtctaacttc
     3181 gattgccata accctgccgg gaattattga tacggaaaat ggtattgtac atcgcatgcc
     3241 gttctacgag gatgtaaaag agatgccgct cggcgaggcg ctggagcagc ataccggcgt
     3301 accggtttat attcagcatg atatcagcgc atggacgatg gcagaggcct tgtttggtgc
     3361 ctcacgcggg gcgcgcgatg tgattcaggt ggttatcgat cacaacgtgg gggcgggcgt
     3421 cattaccgat ggtcatctgc tacacgcagg cagcagtagt ctcgtggaaa taggccacac
     3481 acaggtcgac ccatatggta aacgctgtta ttgcgggaac cacggctgcc tcgaaaccat
     3541 cgccagtgtg gacagtattc ttgagctggc acagctgcgt ctcaatcaat ccatgagctc
     3601 gatgttacat gggcaaccgt taaccgtgga ctcattgtgt caggcggcat tgcgcggcga
     3661 tctactggca aaagacatca ttaccggggt gggcgcgcat gtcgggcgca ttcttgccat
     3721 catggtgaat ttatttaacc cacaaaaaat actgattggc tcaccgttaa gtaaagcggc
     3781 agatatcctc ttccctgtca tctcggacag catacgtcag caggcccttc ctgcgtatag
     3841 tcagcacatt agcgttgaga gtactcagtt ttctaaccag ggcacgatgg caggcgctgc
     3901 gctggtaaaa gacgcgatgt ataacggttc tttgttgatt cgtctgttgc agggttaaca
     3961 ttttttaact gttctaccaa aatttgcgct atctcaattt gggccaggaa agcataactt
     4021 agactttcaa ggttaattat tttcctagtt tatatttgtg aagcataacg gtggagttag
     4081 tgatgctgga gcgtttcttt attaccggta cagacacttc tgtagggaaa acggtggttt
     4141 cccgcgcatt gctacaagcg ttagcctccc agggaaaaac agttgcggga tataaacccg
     4201 tagcgaaggg gagcaaagag acacccgaag ggctgcgtaa taaagatgcc ctggtgttgc
     4261 agagtgtttc aaccatcgaa ctgccttatg aagcagttaa tcctatcgcg ttaagcgaag
     4321 aagaaagtag cgtggcgcac agttgcccaa tcaattacac cctcatttca aacggcctgg
     4381 caaacctgac cgataaagtc gatcatgtcg tggtagaagg gactggcggc tggcgcagtc
     4441 tgatgaatga tttgcgtcca ctctctgaat gggtggtgca ggaacagttg ccggtgttga
     4501 tggttgtcgg tattcaggaa ggttgcatta accatgcact gctaacagct caggcgattg
     4561 ccaacgacgg gctgccgctc attggctggg tggctaaccg aatcaaccca ggactggcgc
     4621 attatgcgga agtcattgat gtgctgggta aaaaacttcc ggcaccgctc attggtgaac
     4681 tgccttatct gccgcgcgcc gaacagcgag aactggggca atacatccgc ttagctatgc
     4741 tgcgcagtgt gctggcggta gatagagtca cggtgtaacg tccgcgaaat taccgacgca
     4801 atgacgcagg caatcaataa accggggagt agctgatact ccccggtcat ttcacatatc
     4861 atcaacgtcg acataatcgg cgcgtgcgtg gttgccgcca acagtgtcgc catcccggtc
     4921 aatccgagta aaagcgtaat ttcttcgcca tcagggaacc ataatcccag gctacgacca
     4981 tacaacatgc caatggcaag accgataaat agcgtcggtg taaagacacc accgggtgcg
     5041 ccggaaccgc tactcgccag cacggcacac agtttacaaa ggaagatccc ggcaatgatc
     5101 attaacagtg gtggggcggt taaaaaggat tgtacggtgc tatagccgtt gccccacact
     5161 gcaggtgtga acagggaaag cagacccacg atcaacccgc ctagtgccag ttgccagggc
     5221 ggcgcaagtt tgagactcac aaatccacga tgacaggcgt tcattaacgt taacaacagt
     5281 ggtccgcaca gacctgccag cacacctgta ctgataatca gcgcatagtc actagcctga
     5341 accgttactg agagttgtac gctgtagagt aacgcgtcgc tatgattaat aagattgcta
     5401 accagcaacg ccacgacggc ggaaataatc accgggccga gagaggccaa catcatagtg
     5461 ccaaacagca cttcggctat aaataaactg ccagcaagcg gggcgcgata ggccgcagcc
     5521 attcccgccg cggccccaca ggcgatccat aatttccatt cctggcgtgg cgtaaaacgt
     5581 tgggcaaaac aggaggcgga aagggcagct aaaagaatca tcgcaccttc gcgaccaatt
     5641 gcactgccgc tggttactac cagcagcgag gcaagcgatt taaccaggct tgctgcgtaa
     5701 tcgaactgtc catcggtttg caacgcttcc atgtaatcgg tcggcgcatg agggcgttgt
     5761 tgggtaaatt tctgccagcc catcagcaac aaacccgccg ccagtccgcc gagcgccgga
     5821 gttagcagcc gtcgccaggg ggaaaggttt gtcgctgcat tgaccagact gccggagtca
     5881 ttattgagga acaaccactc cagtagcagc atcgcatgac gaaacccggc aacggcaaag
     5941 gccgcgagaa taccgacgac tgttgcgata agcagacggc ggaacatagt gcgcagatcc
     6001 ggatagatgt gtagatgatg catgagcaga aatgggcaat gaaacacggg cgactatttt
     6061 gcggcaaaag aggccaaccg caaaagcagt gcgtgcttta agagataaaa aaacgtgaca
     6121 ttgtaactta ttattgccga atcgcgcgta atcgtcacca tccggcaata ttacggtgat
     6181 cctatcgaaa gagtggttta accgcgacag gaatcaacag ttgtgattgc cattgcgcca
     6241 gcgttaagcg agccagttca cccagtgcac ggtagaaagg gtgttctgct ttttcgataa
     6301 aaacatcaag aaaacgtgtt gaccacggaa aaaggtgcca tgccagcaat tcttcgcatt
     6361 ctgtctggcg accattctcc gccaaccacg cagccatcaa cagcagtgaa ccaaaatgat
     6421 cttccggttc gttttgcttc atttcaaact gaatgccttt ctcgcgcatc cactgacgaa
     6481 gtgccaatgt tgaatcgcca aacagcacag attcgcgatc cagccagacc gatccccacg
     6541 gcggagacgg cagtgcccac gggccgacaa acaaacgctg ccaggcctgg gcgtgagtct
     6601 cttcacactg tgtctgaaaa gccgtcacca gcggcgctaa tgacgcttct ggtagaggcc
     6661 actgagtttc ccagccgtca ctggtgagta ccgcaaccag aggtgcggct tccgcactct
     6721 ctggagcgta ataaaacagc gcgcccaaca cgcgcgccgc gacagaaaaa ttatcttgct
     6781 gtgaaaaatg ggtcattcct aacatcctga aatgtgcggg ttgccccgca ccaatttgtt
     6841 aacctgcaat agccattcct actgtcatgt gcaggccata aaagagcaca cgcccgatca
     6901 tctcaccaac aagtatgagg attagcccca gaacaagtcc ggcaacgtga ggttcacggc
     6961 gacgaatgag cggacaaagc cagcatccaa gacctgcgca taacaatacc acacgccaga
     7021 cctgtaagga ggcgtaatct ggtaccagtg cgctggcttg ttgcacggat gagtgaatag
     7081 acgccagaga caaaccttgc agcacaatca ccccggcaca agcgatcagc gcgagaacgc
     7141 taatgatggc aaatggtgtg gtattaaatg taacgcgggc tgcccgcaga attgcggcag
     7201 caagtattgg accgctcaac aataccgtca ggaagaaagc cagcgtagtg taaccgttat
     7261 gccaggttgg cacagtgtcg atttgataca cgcaggtcat catccagacg aaaatgacgc
     7321 cgagcgccat gctgacaagt aaccagagtt tccccaacgc ttgcggcatt ttaccgatga
     7381 ccgccaccag ccaccatagt ccgccaacgg caaaaaatat ggaaccggca gcaatctcgt
     7441 tactcagacc agaagctccg attcgattca gcgagttaaa cgcccgcagt ggcgatccca
     7501 ggtgcatgac agaggccata aacccgatgc ccatcaacag ccagaggaaa aacatgccac
     7561 ggacaatacg ctgacggtct gcgtcatttt tggcggcgaa ccagccaatt ccgctaacaa
     7621 tcagcgcgcc tactacgcat tgacccaata cggtaaagat aaccagtggc cactcatgcc
     7681 atccatttcc cattttacac ttcctccgga ttagccagat aaccagtggt atcgctggtc
     7741 gggcggctgt tggcgttagg tttgataacg atattgggtt ttgtgaaatg cgcgcgcggc
     7801 agtggcgcga cggcagccag agtgccgtgc ttctggcgca gctcttcaat tggaccgaac
     7861 tcaagtgcac gcagcgggca ggattcgaca catatgggtt gtttcccctc ggcgacgcgc
     7921 gaataacaac catcgcactt cgtcatgtgc cctttttcag cattgtactg tggcgcgccg
     7981 tacggacaag ccatgtggca gtagcggcag ccgatacaaa catcttcatc aaccaccaca
     8041 aagccatctt cacgcttatg cattgcgccg ctggggcaga cttttgtaca tgcggggtca
     8101 tcgcaatggt tacaggagat ggagagataa taagcgaaca cgttctggtg ccagacgcca
     8161 ttatcttcct gccagtcgcc gccagcatat tcataaatac ggcggaaact gacttccggg
     8221 ccaagatctt taaaatcttt gcacgccagt tcgcaggttt tacagccagt acagcggctg
     8281 gaatcgataa aaaatccata ttgtgtggtc acgggctact ccttaaacct tttcgatctg
     8341 gacaagattg ctgtgcgacg ggtttccctt tgccagcggt gaagggcggt gagaggtcag
     8401 aatattgata ctgccgccgt gatcgacccg gtcaccaaac atatccgctt taagccaagc
     8461 accttgcccg atggcggtaa cgccaggcag aatacgcgga gtcacttttg cggcaatcag
     8521 catttctcca ttattgttaa atacccgcac ggtatcgcca tgacggatac cgcgtgcctg
     8581 agcatcaatg gggttgatcc acacctcttg tgggcaggcc tgctgtaaca catcaatatt
     8641 gccgtagctg gagtgggtac gcgctttgta atggaagccc gttaactgca gtggataggt
     8701 tttccgcagg ggatcgtccc agccatcaaa acctggggta tacgcaggaa ggggatgaat
     8761 aacttcatct tttttcaatt cccaggtatc cgcaatcttc gccagtcgtt cagaataaat
     8821 ttcgattttc cccgaaggtg ttttcaacgg gtttgcctgt ggatcttcac ggaatgcgcg
     8881 gaaagcgacg tagtgttctt ccgggcattt tttcttaaag atcccggtcg ttttcatctc
     8941 ctcgtagtcg ggcatctcag ggttacgttc cttcgttttc gcatggagat atttgatcca
     9001 ttcatgctga ctgcgacctt cagtaaaggt ttgataaacg tctggtccta agcgtttggc
     9061 gacttcactc agcatccagt agatgggttt gcgttcaaat tttgctgagg ttgcgggttg
     9121 ggcgaggatc acatagccca tattccctgc agattcatga gagataaggt cttcttgctc
     9181 tgttggcatc aggtcgggca acaggatatc gcaatactta gccgaggccg tcatgaagtg
     9241 gtcaatgcca acaatcatct cgcacttgct gtcatcctga agcacctcat gggtgtgatt
     9301 gatgtcgcca tgttgattga tcaatgtgtt actggcgtag caccataaaa acttgatggg
     9361 gacatccagt ttttcttttc cacgaacacc atcacgg
//
LOCUS       DAJTZL010000154         9273 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.154, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000154 DAJTZL010000000
VERSION     DAJTZL010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9273
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.154"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..1220
                     /locus_tag="OO544_003290"
     CDS             <1..1220
                     /locus_tag="OO544_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CS1-pili formation C-terminal domain-containing
                     protein"
                     /protein_id="HCR6768813.1"
                     /translation="YTRTQNNTDDSLRWDKQQSFENNYLRQTTAQSISETVQLSASRA
                     FVMRDWILSTSVGVFHRNDNGGDNDDNGLYLSFSLSDTPTMDSNNNSHSTNVSTDYRY
                     SEQDGDQTSWQLSHTFYNDSFSHKELGVTVGGLNTDTINSAVNGRWDGQYGNVYATVS
                     DSYDRKNHDHLSAFTGTYSSTLAVSCYGVNLGASGTDDLLGAVLVDVKGFSEQDEESQ
                     DLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGFVEVNDSSQGNQQGTTNIINGAGNRE
                     LMLLPGKLRYREVSASFNYNYIGRLLLPAAVKKFPIVGLNSAMLLVAEDGGFTLEING
                     SEKELYLLSGQQFLKCPLSVVKKRASIRYSGDVTCSVVTYSQLPESIQVQAQLKQPKL
                     RGNVQTAQREVAP"
     gene            1217..2305
                     /locus_tag="OO544_003291"
     CDS             1217..2305
                     /locus_tag="OO544_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein CblD"
                     /protein_id="HCR6768814.1"
                     /translation="MRNRLIAAILGLFGTLTGVQAAPDVTSEITYDLASGRADYYFWK
                     DEASAGNNGYMWYECSYPDLQQTCTANGNISTVQIYLTEQRSGMRWPVKLKGFKTAIV
                     SSDEAPPGCKGGKGLQTNLKDSNRSSCTEDGQHYYIYDTKFLTLYLEQTEMKNLPIGG
                     VWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFPEFAHATPRVQLDLHPTGSVNGS
                     NYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAAGRRDGDFSIYRQGGTTTDEGER
                     IDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRPVVLPGIRYAVMCVPTPLTLAVD
                     KFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(2388..3685)
                     /gene="exuT"
                     /locus_tag="OO544_003292"
                     /pseudo
     CDS             complement(2388..3685)
                     /gene="exuT"
                     /locus_tag="OO544_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
     gene            4168..5580
                     /gene="uxaC"
                     /locus_tag="OO544_003293"
     CDS             4168..5580
                     /gene="uxaC"
                     /locus_tag="OO544_003293"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HCR6768815.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            5595..7082
                     /gene="uxaA"
                     /locus_tag="OO544_003294"
     CDS             5595..7082
                     /gene="uxaA"
                     /locus_tag="OO544_003294"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HCR6768816.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINIRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            7165..7716
                     /locus_tag="OO544_003295"
     CDS             7165..7716
                     /locus_tag="OO544_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HCR6768817.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAVDERG"
     gene            complement(7721..8965)
                     /gene="sstT"
                     /locus_tag="OO544_003296"
     CDS             complement(7721..8965)
                     /gene="sstT"
                     /locus_tag="OO544_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HCR6768818.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
BASE COUNT         2430 a         2315 c         2433 g         2095 t
ORIGIN      
        1 gttatacccg cacacaaaat aacactgatg atagtttgcg ttgggataaa cagcagagct
       61 ttgaaaataa ctatcttcgc cagacaacag cgcaaagtat ctctgaaact gtacaactta
      121 gcgcttcccg cgcttttgtg atgcgtgact ggattttgag tacgtcagtc ggtgttttcc
      181 atcgtaatga caacggtggc gataacgacg acaacggctt gtacttatcg ttttcgttat
      241 ctgacacgcc aacgatggat agcaataaca acagccattc aacaaatgtg tctacggatt
      301 atcgttatag cgaacaggat ggcgatcaaa cgtcatggca gttatcgcat accttttata
      361 acgattcatt cagccataaa gaacttggcg taaccgtcgg aggcctgaac accgatacca
      421 taaacagcgc ggttaacggg cgttgggatg ggcaatacgg aaatgtctac gctaccgtat
      481 ctgatagtta tgaccgtaag aatcatgatc atctttcggc ctttacgggc acttatagct
      541 ctacgctggc tgtcagttgc tatggcgtta atttgggagc cagtggtaca gacgatttgc
      601 tgggtgcggt actggtggat gtgaaaggct tctctgaaca ggatgaagag agtcaggatc
      661 tgcaactcga agcgcgggtg gcaggcagcc gaactttgca gcttggtcaa agtgacagtg
      721 tgttgttccc ttatcctgga tttcagtctg gttttgttga ggttaacgac agtagccagg
      781 gcaatcagca ggggacaaca aacatcatta acggtgcagg gaatcgtgaa ttaatgttgt
      841 tgcctggcaa gctgcgctat cgcgaagtgt ctgccagctt taactacaac tatatcggtc
      901 gtttattact gccggcagcg gtgaaaaaat tcccgattgt ggggctgaac agcgccatgt
      961 tactggtagc tgaagatgga ggatttacac ttgaaattaa cggcagcgaa aaagagctct
     1021 atctgctttc cgggcagcaa ttccttaagt gtccactgag tgttgtaaag aaacgcgcca
     1081 gcattcgtta cagcggagat gttacttgta gtgtggtgac ttattcacaa ttaccggagt
     1141 cgattcaggt tcaggcacag ttgaaacagc ctaaattacg tggaaacgtt cagacggcgc
     1201 aaagggaggt tgcaccatga gaaatcgatt gattgcggcg atattgggct tgtttggcac
     1261 gctcactggc gttcaggcag ctcctgacgt gaccagtgaa attacgtatg atttggcatc
     1321 tggtagagcg gattattact tctggaagga tgaagcgtcg gcaggaaata atggatatat
     1381 gtggtatgaa tgttcgtatc ctgacctcca acaaacctgt acagctaatg gaaatatatc
     1441 gacagtacaa atctatttaa ctgaacaacg cagtgggatg cgttggccgg taaaactcaa
     1501 aggatttaaa acagccattg taagtagcga tgaagcgccg ccaggatgca aggggggcaa
     1561 agggcttcag acgaatctta aggattctaa tagatcttca tgtacagaag atggtcaaca
     1621 ttattatata tacgatacaa agtttcttac gctctacctt gagcagacag agatgaagaa
     1681 tttgccgatt ggtggcgtct ggaaggggaa agttaaatta cattcgaaca gcccggccca
     1741 ggactatttc gcaaatatta ccctgaatac gctcgacccc aaccatattg acgtgttctt
     1801 cccggagttc gcccacgcca cgccaagggt gcagttagac ttgcatccaa caggaagcgt
     1861 taacggcagc aactacgcgc aagatctgac catgttggac atgtgcctgt acgatggttt
     1921 taacggtaat gccatcagtt atgaaatcat gctcaaagat gaagggcgac cagctgcagg
     1981 gcgcagagac ggtgacttct ctatctatcg tcaaggaggg accaccaccg acgagggaga
     2041 acgcattgat taccgggtca aaatgtacaa cccggaaacc ggtgggcaaa ttgatgtgcg
     2101 caataatgaa aatatggtct ggaacagcat taacctgaaa cgtgtgcgtc cggtggtact
     2161 gcccggtatt cgctatgccg tgatgtgtgt gccgacaccc ttgactctgg cagtcgataa
     2221 attcagcgtg atggataaac aggccgggta ctacatgggc aaattgtcag taatctttac
     2281 gccttccttg ccaaccatca attgaccccg cagaccagaa aaaactaaac aacgaagccg
     2341 cctgataagg cggcttcgtc attacaactt acgttaaacg ttaaacgtta atgttgcggt
     2401 gcgggatcgt tatgtgtttc ctgcgccacc tcaatcgccg gtttgttctg caatacggtc
     2461 cagataacca gcgcacccag caggtcaaat actgccagaa ctgcgaacag cgggctgaag
     2521 ccgatggtgt cagccagtgc accgacaacc agcgcaaaca gcgtacttgc cagccatgcg
     2581 gacatcccgg ttaaaccgtt cgccgtcgcc acttcgttac gaccgaacac gtcagaagag
     2641 agcgtaatca gcgcgccaga cagtgcctgg tgggcaaaac caccgataca cagcagcata
     2701 attgcgacat acgggttggt gaacagaccg atcatacccg ggccaatcat cagcacagca
     2761 cccagcgtta cgaccatctt acgggaaacg atcaggttca caccaaacca acgctggaac
     2821 agcggcggca ggtaaccacc gaggatacaa ccgaggtcag caaacagcat cggcatccag
     2881 gcgaacatcg cgatctcttt caggttaaag ccgtaaactt taaacatgaa cagcgggatc
     2941 cacgcgttaa aagtacccca ggccggttct gccaggaaac gcggcagtgc gataccccag
     3001 aactgacggt tacgcaggat ctgaccaacg gacattttct tcgccgtgct cacctggtgc
     3061 tgggcttcct gaccattaat aatatagtcg cgttcttcat cggtcagatg cttctggtcg
     3121 cgcggatgtt tatagaaaat cagccatgcc atcgcccaga taaagctcaa tgcaccggag
     3181 atgataaatg ccatctgcca gctgtgcatc acgattgcca taccaccagc ggcggcgcaa
     3241 tcatcgcacc aatcgaagaa cctacgttaa agtagcctac tgcgatggaa cgctctttcg
     3301 ccgggaacca ttcggagctg gctttcagac ccgccgggat catcgctgct tccgcggcac
     3361 cgaccgcacc acgagcaaca gccaggccac cccagctacc tgccagcgcg gttgcaccac
     3421 agaacacggc ccacagcaca gcaaacattg cataaccgat tttcgtaccc agcacatcca
     3481 gtacataacc tgctaccggt tgcatgaccg tataagcagc agaataggct gcgatgatat
     3541 aggaatactg ttgggtggag atgtttaact cttccatcag agttggcgca gctgccgcca
     3601 cagtgttacg cgtcaggtaa ccaagcacgg tgccgagcgt caccagtgcg atcatatacc
     3661 aacgtaaccc tttaatttta cgcatgtaaa acctcatcgt tttgttatat ccgcaggaag
     3721 cgcggccatc atcccgccag aggctgaaga tgttagttac gaccgggtaa ccgaaaaaaa
     3781 accggcacgc cccgaacgtt gccatgaaag tttcgtgcta attcggcttc cgtaccggtt
     3841 aacccgattc atctaacaaa tgaatgaatc ggcaatttgc attccgtcga cttatgattt
     3901 gcgacggcag aaagataact tgtcatacaa ctttaaaagg tgagagccat cacaaatgtg
     3961 cgaatatttg tagggacatt acctgacgac agcgaggcca gtactggcgc ggcctgcagc
     4021 gagatttatc actttgagag taattttttt aactacgttt attgatctaa ctcacgaaaa
     4081 tatcttcgga ctctggaaat tggtgtgata actttgtcag catcgcacca taagcaagct
     4141 agctcactcg ttgagaggaa gacgaaaatg actccgttta tgactgaaga tttcctgtta
     4201 gataccgaat ttgcccgccg tctgtatcac gactacgcaa aagaccagcc gattttcgat
     4261 taccattgcc atttgccgcc gcagcagatt gcggaagact atcgttttaa aaacctgtat
     4321 gacatctggc tgaaaggcga tcactacaaa tggcgcgcta tgcgtaccaa cggtgtggcc
     4381 gagcgtctgt gtaccggtga tgcatctgac cgtgaaaaat ttgacgcctg ggcggcgact
     4441 gttccgcaca ctatcggcaa cccgttatac cactggacgc accttgaact gcgtcgtccg
     4501 tttggtatca ctggcaaatt gctttctccg tcaactgccg atgaaatctg gaacgaatgt
     4561 aacgaactgc tggcgcagga taacttctcc gcgcgcggca tcatgcagca gatgaacgtg
     4621 aaaatggtcg gcaccaccga tgacccgatc gattctctgg agcatcacgc agagatcgcc
     4681 aaagacggct ctttcaccat caaagtgctg ccgagctggc gtccggacaa agccttcaac
     4741 atcgaacagg cgacctttaa cgactacatg gcgaagctgg gcgaagtttc cgataccgac
     4801 attcgccgct ttgctgacct gcaaactgcc ttgaccaaac gtctggatca cttcgccgct
     4861 cacggctgta aagtgtctga ccatgcgctg gacgtggtga tgtttgctga agcgaacgaa
     4921 gcggaactgg acagcattct ggcgcgccgt ctggctggcg aaaccctgag cgagcacgaa
     4981 gtggcacagt tcaaaactgc ggtactggtg ttccttggtg ccgaatatgc acgtcgcggc
     5041 tgggtacagc agtaccacat tggcgcgctg cgtaataaca acctgcgtca gttcaaactg
     5101 ctggggccgg atgtaggctt tgactccatc aacgaccgtc cgatggcgga agagttgtct
     5161 aagctgctga gcaagcagaa cgaagaaaac ctgctgccga aaaccattct ctactgcctg
     5221 aacccgcgcg ataacgaagt gctgggcacc atgatcggta acttccaggg cgaaggtatg
     5281 ccgggcaaaa tgcagttcgg ttccggctgg tggtttaacg atcagaaaga cggtatggaa
     5341 cgtcagatga cccaactggc gcagctcggt ctgctgagcc gctttgtcgg tatgctgact
     5401 gacagccgta gcttcctgtc atacacccgt cacgaatact tccgccgcat tctgtgccag
     5461 atgatcggtc gctgggtgga agcgggcgaa gcaccggcgg acatcaacct gctgggcgag
     5521 atggtgaaaa atatttgctt taacaatgcg cgtgactact tcgccattga actgaactaa
     5581 ggtctggggt tgatatgcaa tacatcaaga tccatgcgct ggataacgtc gcggtcgctt
     5641 tagcagattt ggctgaaggc acagaagtca gtgtcgataa ccagactgtt acgctgcgcc
     5701 aggatgttgc tcgtggacat aaatttgcgt taacggatat cgcaaaaggg gccaacgtca
     5761 tcaaatatgg cctgccgatt ggttatgcat tggcggatat tgcggcgggg gaacacgtcc
     5821 acgcccacaa tacgcgcacg aatctgagcg atctggatca gtatcgctat caacctgatt
     5881 ttcaggatct gcctgcgcaa gcggcagatc gtgaagtgca gatctatcgt cgcgctaacg
     5941 gcgatgtcgg ggtgcgtaac gagctgtgga tcctgccaac cgtgggctgt gtcaacggca
     6001 tcgcgcggca aatccagaac cgtttcctga aagagaccaa caacgccgaa ggtaccgacg
     6061 gcgtgttcct cttcagccac acctacggct gctcacaact gggcgacgat cacatcaaca
     6121 tccgcaccat gctgcaaaac atggtgcgcc acccgaacgc gggcgcagtg ctggtgattg
     6181 gtctgggctg tgaaaacaac caggttgccg cattccgtga aacgttgggc gatatcgatc
     6241 ctgaacgcgt tcatttcatg atctgccaac agcaggatga tgagatcgaa gccgggatcg
     6301 agcatttgca tcagctgtat aacgtgatgc gcaacgataa acgcgagcca ggcaaactca
     6361 gcgaactgaa gtttggtctg gagtgcggtg gttctgacgg tctttctggt attactgcta
     6421 acccgatgct ggggcgtttc tctgactacg tgattgctaa cggcggtact accgtactga
     6481 ccgaagtgcc ggagatgttt ggcgcagagc agttgctgat ggaccattgc cgcgacgaag
     6541 caacgtttga aaaactggtc accatggtca acgactttaa acagtacttt attgcccatg
     6601 atcagccgat ctacgaaaac ccatcaccgg ggaacaaagc gggcggtatc accacgctgg
     6661 aagacaaatc acttggctgt acccagaaag cgggttccag tgtcgtggtt gacgtgctgc
     6721 gttacggcga gcgtctgaaa acgcctgggc tgaacttgtt aagtgcgccg ggtaacgatg
     6781 ccgtagcgac cagcgccctg gcgggtgcgg gctgccatat ggtgctgttc agtactggtc
     6841 gcggcacgcc atacggtgga tttgtgccga cggtgaaaat cgccaccaac agtgaactgg
     6901 cggcgaagaa aaaacactgg atcgactttg acgcgggtca gctgatccac ggtaaagcga
     6961 tgccgcagtt gctggaagaa tttatcgaca ccatcgttga gtttgccaac ggtaagcaaa
     7021 cttgcaacga gcgtaacgac ttccgcgaac tggcgatctt caaaagcggc gtaacgctat
     7081 aaagtcgaaa tgagttgtta ataaaaaacg gcgtttcata atgtgaagcg ccgtttgctt
     7141 ttccatacct gataaggatt tatcatgacc gcgtattggc tggcccaggg cgtgggtgtc
     7201 atcgcctttc tgattggtat cacaacattt ttcaatcgtg acgaacgtcg cttcaaaaag
     7261 cagctttcgg tctatagcgc cgttattggc gtacattttt ttcttctggg cacctatccc
     7321 gctggtgcca gtgccatcct taatgccatt cgtacattga ttaccttacg cacgcgcagc
     7381 ttatgggtaa tggcgatttt tattgtgctg actggcggaa ttggcctcgc gaagttccat
     7441 catcctgtcg aactattgcc ggttatcggt acgattgtca gtacctgggc gctgttccgc
     7501 tgtaaagggc tcaccatgcg ctgcgtgatg tggttttcaa cgtgttgctg ggtgattcac
     7561 aacttctggg cggggtcgat aggcggcaca atgattgagg gtagttttct gcttatgaat
     7621 ggcttgaata tcattcgttt ctggcggatg cagaaaaggg gaattgatcc gtttaaagta
     7681 gagaaaaccc ctcccgccgt agacgaaagg ggttaaacaa ttaattacgc agggcgctat
     7741 ttgccagacg atcgtcttcc gcctggcaag ctgccgcagt gaacagcacg tcagttgaag
     7801 agttcagcgc ggtttcgcaa gagtcctgca atacgccgat gataaagccg acggcaacca
     7861 cctgcatggc gatatcgttc gaaataccga acatattaca ggccagcggg atcagcagca
     7921 gagaaccccc cgccacgccg gatgcgccac aggcacacag agaagccacc acgctcaaca
     7981 gcagcgccgt gggcagatcg accgggatac ccagcgtatt aaccgcagcc agcgtcaaca
     8041 cggtaatggt gattgctgcg cccgccatat tgatggtggc acccagcgga atagaaacgg
     8101 aataggtatc gcgatccaga ttcagctttt cacacagtgc catattcacc ggaatgttcg
     8161 ccgcagagct gcgggtgaag aaggcataca cgccgctttc gcgcaggcac agcagcacca
     8221 gcgggaacgg gttacgacga attttccacc acaccagcaa tgggttaacc accagcgcca
     8281 ccagtaacat acagccaacc agcacgacca gcagttgcgc gtagccccac agtgtggaga
     8341 aaccggtggt tgccagggta gaagaaacca gaccaaaaat accaatcggt gcgaagcgaa
     8401 tgaccagttt caccataaag gtgacggcat tcgacatatc gttgaccagg tttttggtgg
     8461 tctcgttacc gtgacgcagt gcgaaaccga ggccaattgc ccacaccagg atcccgatgt
     8521 agttaccttt cagcagcgcg tcgatggggt tggaaaccat gctcattacc agcccgcgca
     8581 tcacttctac aatgcctgac ggtggcgaaa tatcacccgc gctactggat aagtgcaggg
     8641 tagaagggaa ggcgaagctg aaaacgactg cggccagagc agcagagaag gtacccagca
     8701 gatagaggaa caaaataggg cggatattgg ttttctgccc atgctggtgg ttagcaatag
     8761 atgccatcac cagcatcaac accaggatgg gggcaacggc tttcagtgcg ccgacgaaca
     8821 aagtacctaa cagaccaaca gcttccgccg cgggttttga tatccatgcc agaagaatcc
     8881 ccagaacaag gccgaccagg atttgtttta ccaggctgcc atgagccaga cgccggaata
     8941 gccccggtga acgttgcgta gtcatttttc gatcctttca ttgtgttatc gcacatccct
     9001 ggtgcgttct gtcgacgcat ctttcaggat gtaacaaagt gtttgcaagg tcgagtataa
     9061 ggaaagttac agcttcggga agcgaaaaat gctggatttt aaagagaagt catattttga
     9121 aacttaatgg ttacatttgt ttgacataaa cagagggaag gactggctac taaggctgat
     9181 tagataacat atgggcaaaa ctatttggaa tatgagaacg gttgcgctcc tttaatgtgc
     9241 tatgccatat aaagtcactg gcgcagggag cgc
//
LOCUS       DAJTZL010000155         9217 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.155, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000155 DAJTZL010000000
VERSION     DAJTZL010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9217
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.155"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            73..732
                     /locus_tag="OO544_003297"
     CDS             73..732
                     /locus_tag="OO544_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HCR6768819.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLTEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            836..1810
                     /gene="ghrB"
                     /locus_tag="OO544_003298"
     CDS             836..1810
                     /gene="ghrB"
                     /locus_tag="OO544_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HCR6768820.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLHATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(1860..2570)
                     /locus_tag="OO544_003299"
     CDS             complement(1860..2570)
                     /locus_tag="OO544_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HCR6768821.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            3004..3294
                     /locus_tag="OO544_003300"
     CDS             3004..3294
                     /locus_tag="OO544_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HCR6768822.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            3575..3787
                     /gene="cspA"
                     /locus_tag="OO544_003301"
     CDS             3575..3787
                     /gene="cspA"
                     /locus_tag="OO544_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HCR6768823.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(3975..4127)
                     /gene="hokA"
                     /locus_tag="OO544_003302"
     CDS             complement(3975..4127)
                     /gene="hokA"
                     /locus_tag="OO544_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HCR6768824.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQESYELAAFL
                     ACNLKE"
     gene            complement(4453..6522)
                     /gene="glyS"
                     /locus_tag="OO544_003303"
     CDS             complement(4453..6522)
                     /gene="glyS"
                     /locus_tag="OO544_003303"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HCR6768825.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLDAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(6532..7443)
                     /gene="glyQ"
                     /locus_tag="OO544_003304"
     CDS             complement(6532..7443)
                     /gene="glyQ"
                     /locus_tag="OO544_003304"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HCR6768826.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(7538..7837)
                     /locus_tag="OO544_003305"
     CDS             complement(7538..7837)
                     /locus_tag="OO544_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HCR6768827.1"
                     /translation="MMMNAFFPAMALMVLVGWSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            8012..9007
                     /gene="wecH"
                     /locus_tag="OO544_003306"
     CDS             8012..9007
                     /gene="wecH"
                     /locus_tag="OO544_003306"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HCR6768828.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMAVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(9049..>9217)
                     /locus_tag="OO544_003307"
     CDS             complement(9049..>9217)
                     /locus_tag="OO544_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6768829.1"
                     /translation="MVGLWNATLLLSEKGFYGLAFFLSLFGAVAVQKNIRDAGINPPK
                     ETQVTQEEYSE"
BASE COUNT         2287 a         2331 c         2268 g         2331 t
ORIGIN      
        1 tcatgttcac gcggcataat ctcccgccac ggaacccgtg gcaaagaata aaaaggttat
       61 taaggattaa caatgaagaa acgtgtttat cttattgccg ccgtagtgag tggcgctctg
      121 gcggtatctg gctgcacaac taacccttac accggcgaac gcgaagcagg caaatctgct
      181 atcggcgcag gtctgggctc tctcgtgggc gcgggtattg gtgcgctctc ttcttcgaag
      241 aaagatcgcg gtaaaggcgc gctgattggc gcagcagcag gcgcagctct gggcggcggc
      301 gtgggttatt acatggatgt gcaggaagcg aagctgcgtg acaaaatgcg cggcactggt
      361 gttagcgtaa cccgcagcgg ggataacatt atcctcaata tgccgaacaa tgtgaccttc
      421 gacagcagca gcgcgaccct gaaaccggca ggcgctaaca ccctgaccgg cgtggcaatg
      481 gtgctgacag agtatccgaa aacggcggtt aacgtgattg gttataccga cagcacgggg
      541 ggtcacgacc tgaacatgcg tctctcccag cagcgtgcgg attccgttgc cagcgcgttg
      601 atcacccagg gcgtggacgc cagccgcatc cgtactcagg gccttggccc ggctaaccca
      661 atcgccagca acagcaccgc agaaggtaag gcgcaaaacc gccgtgtaga aattacctta
      721 agcccgctgt aatccctttc atgccaggtg atgcagaaat cacctggcat tcttcagttt
      781 tccttcatca tatttcaggc taaggtgatc gccttatcag tgaatggaga gaagcatgaa
      841 gccgtccgtt atcctctaca aagccttacc tgatgattta ctgcaacgcc tgcaagagca
      901 tttcaccgtt caccaggtgg caaacctcag cccacaaacc gtcgaacaaa atgcagcaat
      961 ttttgccgaa gctgaaggtt tactgggttc aaacgagaat gttgatgccg cattgctgga
     1021 aaaaatgccg aaactgcatg ccacatcaac gatctccgtc ggctatgaca attttgatgt
     1081 cgatgcgctt accgcccgaa aaattctgct gatgcacacg ccaaccgtat taacagaaac
     1141 cgtcgccgat acgctgatgg cgctggtgtt gtctaccgct cgtcgggttg tggaggtagc
     1201 agaacgggta aaagcaggcg aatggaccgc gagcataggc ccggactggt acggcactga
     1261 cgttcaccat aaaacactgg gcattgtcgg gatgggacgg atcggtatgg cgctggcaca
     1321 acgtgcgcac tttggcttca acatgcccat cctctataac gcgcgccgcc accataaaga
     1381 agcagaagaa cgcttcaacg cccgctactg cgatttggat acactgttac aagagtcaga
     1441 tttcgtttgc ctgatcctgc cgttaactga tgagacgcat catctgtttg gcgcagaaca
     1501 attcgccaaa atgaaatcct ccgccatttt cattaatgcc ggacgtggcc cggtggttga
     1561 cgaaaatgca ctgatcgcag cattacagaa aggggaaatt cacgccgccg ggcttgatgt
     1621 cttcgaacaa gagccactgt ccgtagattc gccgttgctc tcaatggcca acgtcgtcgc
     1681 agtaccgcat attggatctg ccacccatga gacgcgttat ggcatggccg cctgtgccgt
     1741 ggataattta attgatgcgt tacaaggaaa ggttgagaag aactgtgtga atcctcacgt
     1801 cgcggactaa gccgcgactg cgtgaagtaa agcccgataa tcgctcgggc ttttactctt
     1861 tattgggttg cagtaactgc tgtagtccag gcctgattaa acgcctgatg ttgtgccggt
     1921 aatggcgcaa tcagtttgtt atattcactt gcctgctgtg aagtcgggaa ctggatgcca
     1981 tttgcgacaa agctcacctg agtaccttgc tgagcaatgt agtctccaac catcaccagt
     2041 tgttgcgtga aagtttgtgc cgccggaatc aacggttgca atgcatcagc tggcgtcgtt
     2101 accactttgg tgaatgcctg atcaaatacc ggtttcaggt catcgctctg ttttagcgca
     2161 gagtgcgcag catcggcctg caacttcgca ttttgcagtt gttgcgccag cacacccagc
     2221 gaaccattca tttcacgcag cggaccgctt tgcgtaacgt aatcctgcgg tacgcgaatt
     2281 gcgttaacgc tgtctacgac cgggcgcaga ccagaatcca tcgcctgatt cacctgctga
     2341 gaataaccat acagaatcgc gtaatcagag acaaaaggac caaactgttt tttctgatcg
     2401 gcagtcaggg ttggtagacg ttcaccgcta cgcatcactg tattctgcag gaagtcgata
     2461 aacgctttac gctgatcgcc ttctttatca aaacacccac tcaggctaac taccattaat
     2521 aacgccgcaa gaggcgcaaa ccagcgagag caggactttc ctgtcgccat tttcttactc
     2581 ctttcaccca aaaaaagcgc acaacgacac acgcgtgtct gacgtgacaa ggatagtcca
     2641 ggacaggctt gcaggatacc ctttctacgt aatcttcctg gtaaaatcat ggagaaaatg
     2701 aaacaaggtt aatttgttgt ttcattgtta aaaaatgata gtgccgttgc cttttttgta
     2761 gccagagatt tacgccttcg cagaaggtta gattttcgaa atctttacaa aacaggagtg
     2821 gtaagagata tatcggcatt tatcagatcg ttaggtggct ataagtcacg ctaaatgaca
     2881 ggctgaatcg aatcatagcc agagcatgcc ctgacttcac cccgctgtgt ctgcttttcc
     2941 cgactattct taatgagctt cgatgcaatt cacgatcccg cagtgtgatt tgaggagttt
     3001 tcaatggaat ataaagatcc aatgcatgag ctgttgagca gcctggaaca gattgttttt
     3061 aaagatgaaa cgcagaaaat taccctgacg cacagaacaa cgtcctgtac cgaaattgag
     3121 cagttacgaa aagggacagg gttaaaaatc gatgatttcg cccgggtttt gggcgtatca
     3181 gtcgccatgg taaaggaatg ggaatccaga cgcgtgaagc cttcaagtgc cgaactaaaa
     3241 ttgatgcgtt tgattcaagc caacccggca ttaagtaagc agttgatgga atagactttt
     3301 atccacttta ttgctgttta cggtcctgat gacaggaccg ttttccaacc gattaatcat
     3361 aaatatgaaa aataattgtt gcatcacccg ccaatgcgtg gcttaatgca catcaacggt
     3421 ttgacgtaca gaccattaaa gcagtgtagt aaggcaagtc ccttcaagag ttatcgttga
     3481 tacccctcgt agtgcacatt cctttaacgc ttcaaaatct gtaaagcacg ccatatcgcc
     3541 gaaaggcaca cttaattatt aaaggtaata cactatgtcc ggtaaaatga ctggtatcgt
     3601 aaaatggttc aacgctgaca aaggcttcgg cttcatcact cctgacgatg gctctaaaga
     3661 tgtgttcgta cacttctctg ctatccagaa cgatggttac aaatctctgg acgaaggtca
     3721 gaaagtgtcc ttcaccatcg aaagcggcgc taaaggcccg gcagctggta acgtaaccag
     3781 cctgtaatct ctgcttaaaa gcacagaatc taagatccct gccatttggc ggggattttt
     3841 ttatttgttt ttcaggaaat aaataatcga tcgcgtaata aaatttagta ttatttttgt
     3901 gaagaataaa tttgggtgca atgagaatgc gcaacgccgt aagaaaggcg ggaataactt
     3961 cccgccaaag actcttactc tttcaaattg caggctaaaa atgccgccag ctcataactc
     4021 tcctgtttaa tatgcaattc acacagtgaa tctcttatca tccaggtgaa aaataaaagc
     4081 gtgaaacaaa tcactattaa agaaagtaat ctatatttct gcggcattcc cagcctcctg
     4141 tgttgatttc caacgagtaa gaggctaacc ttatggtgtt caggcatagg gtagcctcac
     4201 tttgatttat agtcaggtgg ggcttttctc tgtctgcctt tcggtgaata cctgagacaa
     4261 acagtctcaa gcacccgtgg ctattctagc ttaataagtt tgtttcttct ccttgatata
     4321 atcctaaaaa aacctcataa aattaatata tgagataatc tttattcaac agaagattat
     4381 taaaggttgc tgtattattt agcgataata aaaaaagcct gccagatggc aggcttttta
     4441 ataacggcgt tattactgca acagcgaaat atccgcaacg cgcaggaaca gttcgcgcag
     4501 tttctccagc atggtcagac ggttgagacg caattctttg tcatcaacca tgaccatcac
     4561 tttatcgaag aaagcatcaa ccggctcacg cagctctgcc agttcgacca gcgcatcctg
     4621 gtaacgacct tccgcaaagt acggctccag cttgtcacgt agcaccacaa cctgcatcgc
     4681 cagtttaatt tcttccggct ctttcagggt agaggcattc acgcggtcgc tcagcacttc
     4741 gtcagatttc gccagaatgt tagatacacg tttgttcgcc gccgccagtg cagcagctgc
     4801 atccagggta cggaaatgcg ataccgcttt catacgggca tcgaaatcag ccggacgagt
     4861 cggacgacgc gccagtaccg cctggatggt gtcaacggtg taaccttcgt cctgatacca
     4921 ggcgcggaag cgaccgagca taaagtcgat aacatcatca actacgttgg cgttagtcag
     4981 cttatcgcca tacagacgca ctgcttcttc ggtcagcgtt tgcagatcaa ggttgaggtt
     5041 cttctcaacg ataattcgca gcacgccaag tgcggcacga cgcagcgcaa acgggtcttt
     5101 gtcgcctttc ggatgctgac cgataccgaa gatacccgcc agagtatcca tcttatcagc
     5161 aatcgccagc gcacaagcta ccgggttgga cggcaggtca tcaccggcaa agcgcggctg
     5221 atactgctca ttcagcgcga cggctacgtc ttccgcttcg ccatcgtgac gcgcgtagtg
     5281 catccccata acgccttggg tgtcggtgaa ctcgaagacc atgttggtca tcaggtcaca
     5341 cttagacagc aggcccgcac gggttgcgtg gttaacgtca gcgccaatct gttcagcaat
     5401 ccagccagcc agcgcctgga tgcggtcagt tttgtcacgc agcgtaccca gctgttgctg
     5461 gaacagcacg gtttgcaggc gcggcaggtt atcttccaga cgttttttac ggtcggtatt
     5521 gaagaagaac tcggcatccg ccagacgtgg acgaacgact ttctcgttac cagagataat
     5581 ttgctgcgga tctttcgatt cgatgttggc aacgaagatg aagttcggca gcagtttgcc
     5641 atcgttcgca tacaccggga agtatttctg gtcacctttc atggtgtaaa ccagggcttc
     5701 agccggcacc gcgaggaatt tctcttcgaa ttttgcggtc agaacgaccg gccactccac
     5761 cagcgaagcc acttcttcca gcaggctttc gcttaagtca gcgttaccgc caatcttacg
     5821 cgctgcttct tcggcatcgg ctttaatctt cgctttacgt tcttcgtaat cggcgatgac
     5881 tttcccgcgc tcacgcagaa tttccggata ctgatcggcg ttgtcgatgg tgaattccgg
     5941 ttcgcccata aagcggtggc cgcgaatcac gcgatcggac tgaatgccca gaatggttgc
     6001 cggaatgact ttgtcgccca gcagcagggt cacggtgtgc accggacgca cgaagtgcac
     6061 gtcgcttgcg ccccaacgca tcagtttcgg gatcggcagt ttcgccagcg aagtcgcaac
     6121 catattcggc agtagtgctt cagtgctttc gcccttcaca tgggcgcgat acagcagcca
     6181 ttcgccttta tcggtagtca gacgctcagc ctggtcaacg gtaataccgc aaccacgcgc
     6241 ccaaccttct gccgctttgc tcggtttgcc ttcagcgtcg aacgcctggg caatcgccgg
     6301 gccgcgtttt tcgatttcac gatctggttg cgcttccgcc aggttagcta ctttcaacgc
     6361 cagacgacgc ggagcagcaa accattgaac ggtgccgtgt gcgaggccag cgttatccag
     6421 ctccgcagta aagttcgcag caaaggactc agccaggctg cgcagtgctt ttggtggcag
     6481 ctcttcagtg ccgatttcca ccagaaaagt tttctcagac atagccgcct cttacttatc
     6541 tttgttgcac atcgggaagc cgagggcttc acgggaagcg tagtatgctt ctgccactgc
     6601 tttggtcagg gtgcgaatgc gcagaatgta gcgctgacgc tcagtgacgg agatggcttt
     6661 acgcgcatcc agcaggttga agctgtgggc ggctttcaga atacgctcgt aggctggcag
     6721 cggcagcgga ttttccagcg ccagcagctg ctgcgcttct ttctcgtact gctcgaagca
     6781 ggtgaacagg aaatccacat ccgcgtattc gaagttgtaa gtggactgct ccacttcgtt
     6841 ctgatggaac acgtcgccgt aggtggtttt acccagcggg ccgtcgctcc agaccaggtc
     6901 gtaaacgctg tctacgccct gaatgtacat ggccagacgt tccagaccgt aggtgatctc
     6961 gccggtaacc ggtttacact ccagaccacc aacctgctgg aagtaagtga actgcgtcac
     7021 ttccatgccg tttagccaca cttcccagcc cagtccccag gcacccagag tcgggttttc
     7081 ccagttatct tccacgaaac ggatgtcgtg aatagtcggg tccatgccca gctctttcag
     7141 agaaccgagg tacagctcct gaatattgtc cggcgatggc ttaatgacca cctggaactg
     7201 atagtagtgc tgtaaacggt tggggttttc gccgtagcga ccatcggtcg ggcgacgaga
     7261 aggctgaaca taagcagccg ccatcggttc tggccccagc gcgcgcagac aggtcattgg
     7321 gtgagaggtt cccgcgccga cttccatgtc caatggttga acaatggtgc agccctggcg
     7381 agcccagtaa tcctgtaagg tcaggatcaa gccctggaag gtcctggtat caaacttttg
     7441 catattattt cgtgctggat acgtgtggat ttaaaggaag ggatcagtat acccgctgga
     7501 tggaagatat acagtacgaa acgggaaaaa gcagggctta acgcatggaa agatgcaaaa
     7561 aatggccatc tgcgtcaaaa gaacagacaa actgctcttt acggaaggta taaccgcgca
     7621 tttcatagct tccctggaac tgttcgaagg cggtgacgtc gattttttgc tcgccggtgt
     7681 tataacgttt tgccgcctga tccttgcata acgcttccat attcaacgaa cgcagaggat
     7741 caacctttac ccgttgtgct ttctgcacgg gtgacggtgt agaccaaccc actagcacca
     7801 taagcgccat tgccggaaag aaagcgttca tcatcattaa attaccgtct ggtttgcagg
     7861 tgagtcttat tatttatatc ggtatagtgg ctgatatctt acgaatttcc ttaagcctct
     7921 ataagctgta gtgataaaac tcagattatt ccagtgcgcc aaccaaatga tttatcggtc
     7981 agactttttt taaccagata cagagacaca gatgcagccc aaaatttact ggattgataa
     8041 cctgcgaggg atagcgtgtt taatggtggt gatgattcac accactacct ggtatgtgac
     8101 caatgctcat agtgttagcc ccgtcacatg ggatatcgcc aatgttctga attctgcctc
     8161 tcgtgtcagc gtgccgctat ttttcatgat ttccggctat ctcttttttg gcgaacgcag
     8221 cgcccagccg cgccatttct tgcgtatcgg cttatgtctg attttttata gcgcaatcgc
     8281 actgctctac attgcgctgt ttacctccat caatatggag ttagcgctga aaaacctgct
     8341 gcaaaagcca gtgttttacc acttgtggtt tttcttcgcg attgcggtga tttatctggt
     8401 ttcaccgctg attcaggtga agaacgtcgg cggaaaaatg ttgctggtgc taatggcggt
     8461 gattggcatt atcgctaacc caaacacagt gccgcagaaa attgacggtt ttgaatggct
     8521 gccaattaac ttatatatca atggcgatac tttttactac attctgtatg gcatgttggg
     8581 ccgcgctata gggatgatgg acacacagca taaagcactg tcgtgggtga gcgccgcgct
     8641 gtttgcgacg ggggttttta ttatctctcg cgggacatta tatgaattgc agtggcgcgg
     8701 aaattttgcc gatacctggt atctttactg tgggccgatg gtttttatct gcgcaatcgc
     8761 gctattgact ctggttaaaa acacgctgga tacgcgtacc attcgcggac ttggcttaat
     8821 ctcccgccat tcattgggta tatacggatt ccacgccttg attatccatg cgctgcgcac
     8881 ccggggaatt gagcttaaaa attggccaat actggatatt atttggatct tttgcgcgac
     8941 gttggcagcg agtttgttac tttctatgct ggtacaacga atcgacagaa acagattagt
     9001 gagttaagta aaagcccgtt cacattggac tgaccgggct tacgtgaatt attcgctgta
     9061 ttcttcctgg gtaacctgtg tttcttttgg cgggtttatt ccggcatcac gaatattctt
     9121 ctgcaccgct actgcaccaa acaagcttaa gaagaaagcc agtccataaa aacctttttc
     9181 actgagtaat aatgtcgcat tccacagacc taccatc
//
LOCUS       DAJTZL010000156         9188 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.156, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000156 DAJTZL010000000
VERSION     DAJTZL010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9188
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.156"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            171..1769
                     /gene="rtcR"
                     /locus_tag="OO544_003308"
                     /pseudo
     CDS             171..1769
                     /gene="rtcR"
                     /locus_tag="OO544_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417880.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
     gene            complement(1751..2509)
                     /locus_tag="OO544_003309"
     CDS             complement(1751..2509)
                     /locus_tag="OO544_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HCR6768830.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(2526..3356)
                     /gene="glpG"
                     /locus_tag="OO544_003310"
     CDS             complement(2526..3356)
                     /gene="glpG"
                     /locus_tag="OO544_003310"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HCR6768831.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPALKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(3401..3727)
                     /gene="glpE"
                     /locus_tag="OO544_003311"
     CDS             complement(3401..3727)
                     /gene="glpE"
                     /locus_tag="OO544_003311"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HCR6768832.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            3917..5422
                     /gene="glpD"
                     /locus_tag="OO544_003312"
     CDS             3917..5422
                     /gene="glpD"
                     /locus_tag="OO544_003312"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HCR6768833.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDDRM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNTHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGAISDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(5474..6079)
                     /locus_tag="OO544_003313"
     CDS             complement(5474..6079)
                     /locus_tag="OO544_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6768834.1"
                     /translation="MIKFRLYIPPVILGFVIVPLLVWPTVIVLAVLIFTLTFLAEIIF
                     SFPLLVVRISLQELQLELMVEYALFFSVMAGIGWQFSRRTPPELKNRLHCWLVFSPVY
                     FWLILSNFILYISPEKSALLENIRNFFLTFVWPPLNFSPFWPQPWTDFVGPISAQLGF
                     ALGYYCHWCRKNRNQRKKWGDWVTCLSLAILAQGPLFNYLQ"
     gene            complement(6082..6849)
                     /locus_tag="OO544_003314"
     CDS             complement(6082..6849)
                     /locus_tag="OO544_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6768835.1"
                     /translation="MQPSPAMQALIEQIYHTFRRYRVPQKFVVCCEYCLSQQEQKALR
                     STSLRAIPYSLINAWNSSPGPDPQNSDEVRYFLPRLLEFVAQGHFDNIHVVFSLRRIN
                     LANKENWRADERAILQRFACQYMTDWVSGDEAVELQYMLEMFFRADIALAPLLDAINS
                     VPGFWSTVSLACLLNRYCEDYIRDNQDDIDNAITTQINAWARNNHPLLKERARQAIEN
                     PLKQPEPMTEYQVWEDDWIIDECLCAMYDASSESPGK"
     gene            complement(6852..7562)
                     /locus_tag="OO544_003315"
     CDS             complement(6852..7562)
                     /locus_tag="OO544_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6768836.1"
                     /translation="MELVNTLFASLAGTDPFTGVDITIANCKSAYWDEGIVQQLINQA
                     LDEGEKFVGADGLEGLLRYDVTLNIGLTSSKVWPGFSLDTATISRLCACGADFGFDPY
                     ISDVPDVQCDLNTTNDVTVQFTAMLNPDERVIIAKRPLKKCDSWIEDVYIFQVFKDAW
                     KFHNNNSLRGFRDKQAELKLYTRHYSVENCAEESCRDCNSCIRPSFSLSRSALIRLNA
                     ANARFVYQPFTRDQRARG"
     gene            complement(7578..9083)
                     /locus_tag="OO544_003316"
     CDS             complement(7578..9083)
                     /locus_tag="OO544_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001472437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HCR6768837.1"
                     /translation="MQNRKWILSSLVMTFFGIPILTQFLAAVVAMLGVGLAGIIEVCN
                     ILITPTSYLLLNIFMLALGALMLFFSGRVWAGDSAPEKREIAVWRQCLFLVPGLLILV
                     GWIIALHLADYQFHQMGSGWLADLMLPWLGVLLVSVVGGEYWWIVIIPVGAHISFSLG
                     YGRPTRHPLTGTSGLRCRNSLLFILLMLGFVAGYQGYLYKQLNPGVGVRENIDTWAWR
                     PDKLNNQLTPLRGKPQIQFTQNWPRLDGATAAYPIYASAFYALSVIPEDFHTREYLES
                     SRTPDAYNRIVKGDADIIFVAQPSGGQKKRAEESGVTLLYTPFAREAFVFIVNADNPV
                     NSLTEQQVRDIFSGAITNWRTVGGNDQEIQTWQRPEDSGSQTVMQSQVMKNVRMISPQ
                     ETEVASMMEGMIKVVAEYRNTNNAIGYTFRYYATQMNADKNIKLLAINGIAPTAENIR
                     NGKYPYIVDAFMVTRENTTSETQKLLEWFLTPQGQSLVEDVGYVPMYKTLP"
BASE COUNT         2372 a         2359 c         2262 g         2195 t
ORIGIN      
        1 ttgtttgctg tcgtgataat tgcaaccgtc gtgccagaaa attaaataag cagattaatt
       61 ttttaattca ttgtttttaa ataggttaaa tctttacgtc cgtaaacgga gattttccac
      121 aaaaccaatt caccgataat gaaatatcgt cttttataag gatatctaag atgcgtaaaa
      181 cagtggcttt tggctttgtc ggtaccgtgc tggattatgc cggacgcggc agtcagcgct
      241 ggtcgaaatg gcgtccgacg ctttgtttat gccagcaaga atcgttagtc atcgatcggc
      301 tggaattgtt gcacgacact cgctcgcgct cgctattcga aacgcttaaa cgcgatatcg
      361 ccagcgtttc gccagaaaca gaagtcgtgg gcgttgagat tgaactgcat aacccgtggg
      421 atttcgaaga ggtttacgcc tgcctgcatg atttcgcccg tggttacgag tttcagccag
      481 aaaaagaaga ctatttaatt cacatcacca ccggcaccca cgtcgcgcag atttgctggt
      541 ttctgctggc ggaagcgcgt tacctgcccg cccggctgat acaatcttca cctccgcgca
      601 aaaaagaaca gccgcgcggc gcaggtgaag tgacgattat cgatctcgat ttaagccgtt
      661 ataacgccat cgccagccgc tttgccgagg aacgccagca aacgcttgat tttcttaagt
      721 ccggcattgc cacgcgtaac ccccacttca accgcatgat tgagcagatc gaaaaagtgg
      781 cgatcaaatc ccgcgcgccg attctgctta acggtccaac cggcgcgggc aagtcatttc
      841 tggcgcgacg catcttcgag ttaaaacagg cgcggcatca gtttagcggc gcgtttgtgg
      901 aagtgaactg cgccaccctg cgcggcgata ccgccatgtc gacgctgttt ggtcatgtaa
      961 aaggcgcgtt taccggggcg cggtaatctc gtgaaggttt attacgcagc gccaacggcg
     1021 gaatgttgtt tcttgatgag attggcgaac tgggcgcaga cgaacaggca atgctgctaa
     1081 aagccattga agagaaaacc ttttacccgt ttggcagcga tcgccaggtg agcagtgatt
     1141 ttcagcttat cgccggaacg gtgcgcgatt tgcgccagct ggttgccgaa ggcaaatttc
     1201 gcgaagatct gtacgcgcgg atcaatctct ggaccttcac cctgccgggt ctacgccagc
     1261 gccaggaaga tattgaaccg aaccttgatt atgaagtgga gcgccacgcc acactcactg
     1321 gcgacagcgt gcgttttaac accgaagcgc ggcgcgcctg gttggctttt gcgacctctc
     1381 cccaggcaac atggcgcggt aactttcgcg aactttctgc cagcgtcacg cggatggcca
     1441 cctttgccac tagcggacgc atcactctgg acgtggttga agatgagata aaccgtctgc
     1501 gctataactg gcaggagagc cgcccctccg cgcttacggc gttgctgggc gcagaggcag
     1561 aaaacatcaa tctcttcgac cgtatgcaac tggaacacgt tatcgctatc tgccgctagg
     1621 caaagtcgct ttctgcagcc ggacgacagc tttttgatgt ttcgcgccag ggcaaagcca
     1681 gcgtcaatga cgcggatcgg ctacgcaaat acctggcgcg ttttggtctg acgtgggaag
     1741 ccgtgcagga tcagcacagc tccagttgaa tatgatggtc cgtcagcacc tgcattacgc
     1801 ttactggcgg cggggcgtcg gtgtagacgg catctaccat gctgatgctg cccatattga
     1861 ccatcgcgtt acggccaaat ttcgagtgat cgacaaccag cataacgtgg cgcgagttct
     1921 caataatggc gcgcttggtg cgaacttcgt ggtaatcgaa ctccagcagc gagccgtcgc
     1981 tatcgatgcc gcttatcccc agaatgccga aatcaaggcg gaactgggag ataaaatcga
     2041 gcgtcgcttc gccaatgatc ccgccatcgc ggctgcgtaa ttcgccaccg gcgagaataa
     2101 tgcggaaatc ttcttttacc atcaacgtgt tagcaacatt gagattgttg gtgacaatac
     2161 gcaaattgct gtgattaagc agcgcgtgcg ccaccgcttc cggcgtggtg ccgatatcga
     2221 taaacagtgt cgagccgttg gggatttgct ccgccacttt gcgggcaatc ctctcttttt
     2281 cttcggtctg ggtggctttg cgatcgtgcc acggcgtgtt aaccgaactg gaaggcagcg
     2341 ccgcaccgcc atggtgacgc aggatcaggt tttgctccgc cagctcattg aggtcgcggc
     2401 gaatagtctg cgggctgacg gagaaatgct ctaccagctc ttcagtactg acataaccct
     2461 gctgtttaac cagttcgata ataccgttgt gacgttgtgt ttgtttcatt tataaatccc
     2521 tggaattatt ttcgttttcg cgcattgagc gaatcaacaa aagccatcgc taaacccacg
     2581 gctaacccgg cgatgtgtgc tccgttcgcc atcgacatcc caaacaaatc aaaccatccg
     2641 gcgacaatcc agatcagcgc aaagataatt aacccacgtt gcaggtaaat gccactttgc
     2701 ggatcgcgtt caccacgtag ccagacgtag cccatcagcg catacaccac gccagaaagc
     2761 ccgccaaacc acggcccgct gaatttttgc tgcacatagc cgcttaacag ggcgctaatg
     2821 agagtaataa caattagctt accgctaccg aggcgttttt ccaccgcacc gccgagatac
     2881 caccaccaga gcaggttaaa gaggatatgc atcagcgaga agtgcattaa cgcgtgggtg
     2941 aagtaacgcc agaactcaaa tttcagtgct ggatcgaatg gccaggccag ccataacatc
     3001 acttcctgat cgccgagaat ttgcatggca ataaacacca ccacgcaggc gatcatcatc
     3061 acccaggtca ccggacctgc gcgttcacgc aaggcggcaa agaaaggata acggcgataa
     3121 tgcaggccac tgccggtatg gcctgcctgc cagctggccg ccagataacg cggatctgcc
     3181 gggttttcga gaaaacgcgc cagctccgcc cgtacgcgct cggcctggga ctcatccgcc
     3241 agccagacat cgctttggtt atgttgttga atcgtgagga taacaccctg cgtcgccatg
     3301 taatcaacaa acgcctgcgc cacgcggggg ttagcaaaag aggtaatcat caacatcgtt
     3361 gctgtcgctt attccacaca aaaggggaca gtataaagcg ttacgcgccg tacgccacct
     3421 ctgcgggaaa ctgacgttgc caggcttcaa agccgccgtc aatgctatag accacatcgt
     3481 agccctgttg cagcagatac tgcgccgcgc ctttgctgct attgccgtga taacacatca
     3541 ccatcaccgg agtgtcaaag tcgttatcac gcataaacgc gcccagcgtg tcgttggtta
     3601 aatggaaagc ctgcaccgca tgccccatag cgaaactctg tggatcgcga atatcgacca
     3661 gcaccgcctc tttttcctgc aacttctggt gcgcgtcggc aacgttaata cattcgaact
     3721 gatccatgcg tctctctttc tttacaaaca agtgggcaaa tttaccgcac agtttacgtc
     3781 gaagcggcag ataaacgcca taatgttata catatcactc taaaatgttt tttcaatgtt
     3841 acctaaagcg cgattctttg ctaatatgtt cgataacgaa catttatgag ctttaacgaa
     3901 agtgaatgag ggcagcatgg aaaccaaaga tctgattgtg ataggtggcg gcatcaatgg
     3961 tgctggtatc gcggcagacg ccgctggacg cggtttatcc gtgctgatgc tggaggcgca
     4021 ggatctcgct tgcgcgacct cttccgccag ttcaaaactc attcacggtg gcctgcgcta
     4081 ccttgagcac tatgaattcc gcctggtcag cgaggcgctg gctgaacgtg aagtgctgct
     4141 gaaaatggct ccgcatatcg ccttcccgat gcgttttcgc ctgccacatc gtccgcatct
     4201 gcgcccggcg tggatgattc gcattggtct gtttatgtac gatcatctgg gtaaacgcac
     4261 cagcctgccg ggatcaactg gtttgcgttt tggcgcaaat tcagtgttaa aaccggaaat
     4321 taagcgcgga ttcgaatatt ctgactgttg ggtagacgac gcccgtctgg tactcgccaa
     4381 cgcccagatg gtggtgcgta aaggcggcga agtgcttact cggactcgcg ccacctctgc
     4441 tcgccgcgaa aacggcctgt ggattgtgga agcggaagat atcgataccg gcaaaaaata
     4501 tagctggcaa gcgcgcggct tggttaacgc caccggcccg tgggtgaaac agttcttcga
     4561 cgacaggatg catctgcctt cgccttatgg cattcgcctg atcaaaggca gccatattgt
     4621 ggtgccgcgc gtgcataccc aaaagcaggc ctacattctg caaaacgaag ataaacgcat
     4681 tgtgttcgtg atcccgtgga tggacgagtt ttccatcatc ggcaccaccg atgtggaata
     4741 caaaggcgat ccgaaagcgg tgaagattga agagagtgaa atcaattacc tgctgaaagt
     4801 gtataacacg cactttaaaa agcagttaag ccgtgacgat atcgtctgga cctactccgg
     4861 tgtgcgtccg ctgtgtgatg atgagtccga ctcgccgcag gctattaccc gtgattacac
     4921 ccttgatatc catgatgaaa acggcaaagc accgctgctg tcggtattcg gcggtaagtt
     4981 gaccacctac cgcaaactgg cggaacatgc gctggaaaaa ctgacgccgt attatcaggg
     5041 cattggcccg gcatggacga aagagagcgt gctgccgggt ggtgccattg aaggcgaccg
     5101 cgacgattac gcagctcgtc tgcgccgtcg ctatccattc ctgactgaat cgctggcgcg
     5161 tcattacgca cgcacttacg gcagcaacag cgagctgctg ctcggcaatg cgggggcgat
     5221 aagcgacctc ggggaggatt tcggtcatga gttctacgaa gcggaactga aatacctggt
     5281 ggatcacgaa tgggtccgcc gcaccgacga tgccctttgg cgtcgcacaa aacaaggcat
     5341 gtggctgaat gcggatcaac aatctcgtgt gagtcagtgg ctggtggaat atacgcagca
     5401 gaagttgtcg ctggcgtcgt aaggatgatg cccggttgaa gtgcaaccgg gctatttgga
     5461 ttccttagac tattcattgt aaataattga ataacggccc ctgagctaaa atcgccaaac
     5521 ttaagcacgt tacccaatcg ccccacttct tcctctggtt tctatttttt ctgcaccaat
     5581 ggcaataata tcccaatgca aaaccaagct gtgcactaat cgggccgaca aaatcagtcc
     5641 aaggctgcgg ccaaaaaggg gaaaaattca ggggaggcca gacaaacgtc agaaagaaat
     5701 ttcggatatt ttccagcaac gctgatttct ctggagaaat ataaagaatg aaattcgaga
     5761 gaattaacca gaaatagacc ggcgaaaaga ccagccagca atgcagtctg ttttttaatt
     5821 caggaggcgt tctgcgggag aattgccaac cgatgccagc cattacactg aaaaaaagtg
     5881 catattcaac cattaactca agttgtaatt cctgaagaga aatacgcaca accaggagcg
     5941 gaaaggagaa tattatttct gccagaaaag ttaacgtgaa tataagcacg gctaagacaa
     6001 taaccgtcgg ccataccaat aatggcacga taacaaaacc gagaattaca gggggaatat
     6061 aaagccggaa tttaatcatt tttattttcc tggtgattcg gatgatgcgt catacatcgc
     6121 gcataaacac tcatcaatta tccagtcatc ttcccagacc tgatattctg tcattggttc
     6181 gggctgtttt agcgggttct caatcgcttg ccgcgctctt tctttcagta aaggatgatt
     6241 atttctagcc caggcattga tttgggtggt aatggcgtta tcgatatcgt cctggttatc
     6301 tctgatataa tcctcacaat atctgttcaa taaacaggca agagaaacgg tcgaccaaaa
     6361 gccgggaact gaattaatgg catccagcaa tggcgcaaga gcgatatcgg cacggaaaaa
     6421 catttccagc atatactgta attcgacagc ttcatcgccg gatacccaat cagtcatata
     6481 ctggcaggca aatcgctgta aaattgcccg ttcatccgca cgccagtttt ctttgttggc
     6541 aaggttgata cggcgcagag aaaaaactac gtgaatattg tcaaaatgcc cctgtgcaac
     6601 aaattccagt aaacgcggca gaaaatagcg gacctcatcg ctattttgcg gatcaggccc
     6661 tgggctacta ttccaggcat tgattaagga atacgggatc gctcgtaacg atgtactccg
     6721 taatgccttt tgttcctgct gactcaggca atattcacaa caaaccacaa atttctgcgg
     6781 cacccgataa cgacgaaagg tgtgataaat ctgctcaatt aatgcctgca tggcggggct
     6841 gggttgcata gttaccccct cgcccgctga tcgcgtgtaa acggttgata gacaaaacga
     6901 gcattcgccg cgttcaggcg aataagggcg ctacgcgaaa gggaaaagct ggggcgaatg
     6961 caactattgc agtctcgaca gctttcctct gcgcaatttt ccactgagta atgacgcgtg
     7021 tagagtttca gttcggcctg cttatcgcga aatccgcgta aactgttatt gttatgaaat
     7081 ttccaggcat ctttaaagac ctgaaagata tagacatcct caatccagga gtcacatttt
     7141 ttaagcgggc gctttgctat tatcacccgc tcatccggat ttagcatcgc cgtaaattgt
     7201 acggtgacgt catttgtcgt gttcaggtca cattgaacat ctggtacatc actgatatag
     7261 gggtcaaatc caaaatcagc accacaggca caaagacggc taatggtggc ggtatcgagg
     7321 gaaaatcccg gccatacctt gctggaggtt aagccaatat ttaatgtgac atcatatcga
     7381 agcaatccct caagaccgtc agcaccgacg aacttttccc cttcatccaa ggcctggtta
     7441 ataagttgct gaacaatgcc ttcatcccag tatgccgatt tgcagttggc aatcgtaata
     7501 tccacgcctg tgaagggatc agtaccggca aggcttgcaa ataatgtatt aactaattcc
     7561 atttttatta tccagcttta aggtagcgtc ttatacatcg ggacataacc tacatcttcc
     7621 accaaactct gtccctgcgg cgttaaaaac cactccaaca atttttgtgt ctctgacgtc
     7681 gtattttccc gcgttaccat aaaggcatcg acgatatagg gatatttgcc gttgcgaatg
     7741 ttttcggcag tcggtgcaat accgttaatc gctagcagtt ttatattttt atcggcgttc
     7801 atttgtgttg cgtaataacg gaaggtgtaa cctattgcgt tgtttgtatt acggtattcg
     7861 gcaacgactt taatcattcc ctccatcata cttgccactt ctgtttcctg cggcgagatc
     7921 atgcggacat ttttcatgac ctgggattgc atcaccgtct ggctaccaga gtcttccggg
     7981 cgttgccagg tctggatctc ctgatcgtta ccgccaacag tacgccagtt ggtaattgca
     8041 ccactgaaga tgtcacgcac ctgttgttca gtcagggaat taaccggatt atccgcattg
     8101 acgatgaaaa caaacgcttc acgggcaaaa ggcgtgtaca gcaaagtaac gcccgattcc
     8161 tcagcgcgtt ttttctgccc accggaaggt tgcgccacaa aaataatatc agcatcacct
     8221 ttaacaatcc tgttatacgc atcgggggta cgagagctct ccagatattc cctggtgtga
     8281 aaatcttccg gtataacgct taaagcataa aatgcagagg cataaattgg gtacgccgcc
     8341 gtagcaccat cgagtcgcgg ccagttttgc gtaaactgaa tttgcggttt accacgcagt
     8401 ggtgtcagtt gattattgag tttatcgggt cgccaggccc aggtatcaat attttcgcgc
     8461 acaccgacac cgggattgag ctgtttatat aaataaccct gataaccagc gacaaaacca
     8521 agcatgagaa ggataaacag gagggaatta cggcaacgta gcccggatgt accggttaaa
     8581 ggatgtctgg ttggccggcc gtaccccagc gaaaaactga tatgcgcgcc aacgggtata
     8641 atgactatcc accagtactc acctccgact actgagacta acaaaacgcc cagccagggg
     8701 agcataagat cagccaacca acccgatccc atctggtgaa attgataatc tgccagatgc
     8761 agcgcgatta tccagccaac caggattaat aaaccgggta ctaaaaaaag acattgtcgc
     8821 cagacggcta tttctctttt ttctggtgca ctatcgcctg cccatactcg ccccgagaaa
     8881 aatagcatta atgcgcccag cgccagcata aaaatgttga gcagaaggta acttgttggc
     8941 gtgataagga tattacaaac ttcaataata ccggcaagtc cgacacccag catagcaaca
     9001 accgccgcca aaaattgcgt cagtatgggg atgccgaaaa aagtcattac cagcgaggac
     9061 aaaatccatt ttctgttttg cattattctt tccattcttt ttgaatggtg aaattatact
     9121 ccccgagtcc ccttgcccct tctggacact tttccgaaat gatggcggaa aaaaacggga
     9181 ccctttgg
//
LOCUS       DAJTZL010000157         9059 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.157, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000157 DAJTZL010000000
VERSION     DAJTZL010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9059
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.157"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..781
                     /gene="umuC"
                     /locus_tag="OO544_003317"
     CDS             <1..781
                     /gene="umuC"
                     /locus_tag="OO544_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HCR6768838.1"
                     /translation="VDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLA
                     DTDIRFIRKHFNVVLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQ
                     AICSYAARAAEKLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDII
                     NAATRSLDAIWQAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMTVMDTLN
                     AKEGRGTLYFAGQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(827..1357)
                     /gene="dsbB"
                     /locus_tag="OO544_003318"
     CDS             complement(827..1357)
                     /gene="dsbB"
                     /locus_tag="OO544_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HCR6768839.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGLEMPQWLLGIFIAYLIVAVLV
                     MISQPFKAKKRDLFGR"
     gene            complement(1503..3044)
                     /gene="nhaB"
                     /locus_tag="OO544_003319"
     CDS             complement(1503..3044)
                     /gene="nhaB"
                     /locus_tag="OO544_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HCR6768840.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAIFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     IGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            3265..3984
                     /gene="fadR"
                     /locus_tag="OO544_003320"
     CDS             3265..3984
                     /gene="fadR"
                     /locus_tag="OO544_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HCR6768841.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(4036..5568)
                     /locus_tag="OO544_003321"
     CDS             complement(4036..5568)
                     /locus_tag="OO544_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HCR6768842.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREMRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            5898..7196
                     /gene="dadA"
                     /locus_tag="OO544_003322"
     CDS             5898..7196
                     /gene="dadA"
                     /locus_tag="OO544_003322"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HCR6768843.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            7206..8276
                     /gene="dadX"
                     /locus_tag="OO544_003323"
     CDS             7206..8276
                     /gene="dadX"
                     /locus_tag="OO544_003323"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HCR6768844.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISSAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALVLRVPVVTV"
     gene            complement(8663..>9059)
                     /gene="cvrA"
                     /locus_tag="OO544_003324"
                     /gene_synonym="nhaP2"
                     /gene_synonym="ycgO"
     CDS             complement(8663..>9059)
                     /gene="cvrA"
                     /locus_tag="OO544_003324"
                     /gene_synonym="nhaP2"
                     /gene_synonym="ycgO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415709.2"
                     /note="the Vibrio parahaemolyticus gene VP2867 was found
                     to be a potassium/proton antiporter; can rapidly extrude
                     potassium against a potassium gradient at alkaline pH when
                     cloned and expressed in Escherichia coli; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="K+/H+ antiporter"
                     /protein_id="HCR6768845.1"
                     /translation="RDNQLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVAL
                     DQRFFGDFILEASAKYADVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEF
                     AGMIWTVAEKEDNEVLKIGVRVAEEEAES"
BASE COUNT         2269 a         2298 c         2397 g         2095 t
ORIGIN      
        1 ggtggattta tcaaatctgg aacgccagcg taaattaatg tctgctctcc ccgtggatga
       61 cgtctggggg attggacggc ggatcagcaa aaaactggac gcgatgggga tcaaaaccgt
      121 tcttgatttg gcggatacag atatccggtt tatccgtaaa cattttaatg tcgtgctcga
      181 aagaacggtg cgtgaactgc gcggtgaacc ctgtttgcaa ctggaagaat ttgcaccgac
      241 aaagcaggaa attatctgtt cccgctcgtt tggtgaacgt atcacggatt atacgtcgat
      301 gcggcaggcc atttgtagtt acgctgcccg ggcggcggaa aaacttcgca gtgagcatca
      361 atattgtcgg tttatctcca catttattaa gacgtcacca tttgcgctca atgaacctta
      421 ttacggcaat agcgcgtcgg taaaactgct gacgcccact caggacagca gggatatcat
      481 taacgccgct acgcgatctc tggatgccat ctggcaagcg ggccatcgct accaaaaagc
      541 gggcgtgatg ctgggggatt tcttcagtca gggagtcgcg cagctcaatt tattcgatga
      601 caacgcaccg cgccccggga gtgagcaatt gatgacggta atggatacac tgaatgctaa
      661 agagggcaga ggaacactct attttgccgg gcaggggatc caacaacaat ggcagatgaa
      721 gcgagccatg ctttcaccac gttatacaac gcgaagttct gatttactgc gggtcaaata
      781 aatatagcgg caggaaaaaa gcgctcccgc aggagcgccg aatggattag cgaccgaaca
      841 gatcacgttt tttcgcttta aacggctggg aaatcatcac cagcaccgcg acaatcaggt
      901 aagcgataaa aataccgagc agccactgcg gcatttccag acctaaaaaa tcccactgac
      961 gctcggcgca atcgccagag gcgacaaaca cttgcggcac ccatttatcc agcggcagcc
     1021 attccgggaa acgaaccata aaatcacagg tggcaaacgg cgaaggatag agctgaagca
     1081 tggtgtgctc gtaagttaac tgcacaccgc ggaacgcact atacaaccag ataaccatcg
     1141 ctacataacg cagcggagtt ttcggggcga tcgcgccaat cagcgcagca cccagaacgc
     1201 cgaataacgc gcagcgttca taaatacaga gcacgcaagg tttcagtaac atcacatgct
     1261 ggaaccacag cgccgtcagt tccagtgcca gagcagtaaa cgccatcaac agccacgcgc
     1321 cccggccttg tgaacattgg ttcaaaaatc gcaacataat catttccctg caatatgcat
     1381 agagtgcgca gtttaaacca attcattcgc tgcgccacca ggaggcagca aaaataggac
     1441 gcaattggac aattatcggg cgaaaaggca aaccgggcat ttatatgccc ggtaagttgt
     1501 tatcaaagcg ttgctatcca gcccatttgc ataaaccatt cggttacagg ggcaagcgta
     1561 aactcgacgc agagcagtcc gacgagtgtc aggacgaggg tgtaaggcag ggccatccac
     1621 accatgcggc cataagagag gcgaatcaat ggcgcgagtg cagaggtcag caggaatagg
     1681 aacgcagcct gaccgttcgg cgtagcgaca gagggcagat tggtaccggt attaatggcg
     1741 accgccagca gctcgtattg cttcaacgtg atagcgccac tttccattgc cgctttcgct
     1801 tcgttgatat aaatcgtccc cacgaagacg ttatccgaaa tggatgacag cagaccgttg
     1861 aatatataga acagcgacag ctgagcatgt tccgatgcct gcaacacaaa ctgaataatc
     1921 ggcgaaaaca gttgttggtc gataatcacc gcgactaccg agaaaaacac cgtcaacagt
     1981 gcggtgaatg gcagagattc ggtgaaggct ttaccgatag catgctcatc ggtgacaccg
     2041 atcaatgatg tcgccagaat aatgactgac aaaccaatca aaccaacttc cgccagatgc
     2101 aacgccagcg cagtcaccaa ccagacgcca ataatcgcct ggacaatcag acggatttta
     2161 tcctgacggg tacgctggtg gcggctttga tcgtcaaact gctgcagcac ttcgcggact
     2221 ttctccggca gcgtttcacc gtagccaaac caacgcagct tctccaccag caggcaggtt
     2281 aacaggccac aaatcagaac cggaacggtc accggcgaca tgcgcaggaa gaaatcgcca
     2341 aaatgccagc cagccgcttt agcgatgatc aggttctgtg gttcgcccac catggtcatc
     2401 acgccgccta atgcggtgcc gacaccggca tgcatcatca ggctacgcag aaagccacgg
     2461 aactgttcca gaaccacttt gtaatgcttg tcgatatgac tatcgtcttg caggtcggta
     2521 tcttcagtac gggaagaggc tacgcggtga taaataccat aaaaaccgac tgcaacgctg
     2581 atcaccaccg ccacgacggt taaggcatcg aggaacgcgg agaggaacgc cgccgccacg
     2641 caaaaagaga gcgacagcag cattttggag cgaatgctta acagcaaacg ggtaaatatg
     2701 aacagcaata gctgtttcat aaaatagata cccgccacca taaacatcag taacagcaag
     2761 acttcaagat ttgccgccac ctcttcacgg acgtgttccg cgctggtcat gccgatgaat
     2821 atcgcttcga tagccaacag accaccgggg agcagcgggt agcatttcag ggccatcgcc
     2881 agagtgaaaa taaattccgc taccagcaac cagcccgcga cgaaagggct gatgaggaaa
     2941 attaacgggt ttacgattaa gaaaatgatg agggcgagtt tgtaccagtc gggggactgg
     3001 cccaaaaaat tgcgccatag cgcgcggccc caggagatct ccatgatggt ttcccttacc
     3061 ttacaaataa tcaatgatgt ttttatgttt aaacgcaaag cttaacggtc aggcaggagt
     3121 gaggcaagtc ttgatagtca aggggaaaga gatgcggaaa atgaagcctt gatccctttt
     3181 tcttcttttt gtctgctatc agcgtagtta gccctctggt atgatgagtc caactttgtt
     3241 ttgctgtgtt atggaaatct cactatggtc attaaggcgc aaagcccggc gggtttcgcg
     3301 gaagagtaca ttattgaaag tatctggaat aaccgcttcc ctcccgggac tattttgccc
     3361 gcagaacgtg aactttcaga attaattggc gtaacgcgta ctacgttacg tgaagtgtta
     3421 cagcgtctgg cacgagatgg ctggttgacc attcaacatg gcaagccgac gaaggtgaat
     3481 aatttctggg aaacttccgg tttaaatatc cttgaaacac tggcgcgact ggatcacgaa
     3541 agtgtgccgc agcttattga taatttgctg tcggtgcgta ccaatatttc cactattttt
     3601 attcgcaccg cgtttcgtca gcatcccgat aaagcgcagg aagtgctggc taccgctaat
     3661 gaagtggccg atcacgccga tgcctttgcc gagctggatt acaacatatt ccgcggcctg
     3721 gcgtttgctt ccggcaaccc gatttacggt ctgatcctta acgggatgaa agggctgtat
     3781 acgcgcattg gtcgtcacta tttcgccaat ccggaagcgc gcagtctggc gctgggcttc
     3841 taccacaaac tgtcggcgtt gtgcagtgaa ggcgcgcacg atcaggtgta cgaaacagtg
     3901 cgtcgctatg ggcatgagag tggcgagatt tggcaccgga tgcagaaaaa tctgccgggt
     3961 gatttagcca ttcaggggcg ataatccctt ccgtttaaag agcaaacccc tcaaacgggg
     4021 ggttttttgt tgtttttaca gatttcccat tcttggcggg caacgttcca gcaactcgat
     4081 gctgccgtct tcgttttgct gttcgagcat cacatcaaat ccccacaggc gatgcacatg
     4141 cttcaggact tctttgcgcc cccgatccag cggtgcgcga ttatgtggaa tataacgcag
     4201 cgtcagcgaa cggtcgccgc gcaaatccac gttccagatc tgaatattcg gctccagatt
     4261 acttaagtta tattgcgacg ataaccggtt acgcatctcc cgataacctt cttcattatg
     4321 aatagcggaa atctccagat aattatgccg atcatcatcc agcacggtga agaagcggaa
     4381 atcacgcatc actttcggtg acagaaactg gctgataaaa ctttcatctt tgaaatcacg
     4441 catcgcgaaa tgcagcgttt ccagccagtc ggaaccggcg atatctggga accagtattt
     4501 gtcttcttcc gttggcgact gacaaatccg tttaatatcc tggaacatgg cgaacccgag
     4561 ggcatacggg ttgatgccgc tgtaccacgg gctgttatag gggggctgga agaccacatt
     4621 ggtgtggctg tgcaaaaact ccagcataaa acgttccgtt actttccctt catcatacag
     4681 atggttaagg atggtgtagt gccagaaggt cgcccagcct tcgttcatca cctgagtctg
     4741 tttttgcgga taaaaatact ggctcacctt acgcacaata cgcaggattt cacgctgcca
     4801 tgattccagc agtggcgcat ttttctccat aaaatagagc aggttttctt gtggttcgga
     4861 cggatagcgg cgcgcttcag caaccgtttt ctcttcctcg cgcttcggca gagtacgcca
     4921 gagcatattg acctgacttt gcagatactc ttcgcgactt ttctgccggg ctttctcttc
     4981 ttgcagcgag attttttgcg ggcgtttgta ccggtccacg ccgtagttca tcagcgcgtg
     5041 gcacgagtcc agaagccgtt ctacttcatc cacgccataa cgttcttcgc actcggtaat
     5101 atatttacgg gcaaaaatca gataatcgac aatcgaactg gcgtcggtcc aactgcggaa
     5161 taagtaattg tttttaaaga aagagttatg cccatagcag gcgtgtgcca tcaccagcgc
     5221 ttgcatggta atggtgttct cttccatcag gtaagcgata cacgggttag agttaatgac
     5281 gatttcatag gccagccctt gctgaccgtg cttatacagc cgttcagtct cgataaattt
     5341 tttaccgaat gaccagtgcg gatagttaat tggcatgccg acgctggagt aggcatccat
     5401 catctgttct gaggttatca cttcaatctg gtgcgggtag gtatccagcc ggtagagttt
     5461 cgccacccgg tctatctctg ccagataaac atccagcagg tcgaacgtcc agtcgggtcc
     5521 atcgctcaaa cgtgtggtgt ccttattcat agaatcgatc gtcgccatac gcgcacctca
     5581 ttgttgtcgg cgctctctgt gtggagcacc tcatttcaag catagaacac ctgttaaaaa
     5641 ccgcgtcgcc ggagaatttt tttctttgcg atttcttatt atcagagtgc cactaatccg
     5701 cttctgaacg gaattttatg ctggataaaa agggcgttca gcaggagata ctaaagacgc
     5761 catattgccg cagagtcagg gagatgtgag ccagctcacc ataaaaaagc cgtatgttga
     5821 ataatgtttt caactgagtt atcaagatgt aattagatta ttattctttt actgtatcta
     5881 ccgttatcgg agtggctatg cgagttgtca tactgggaag tggtgtggta ggcgttgcca
     5941 gcgcctggta cttaaatcag gcaggacatg aggtcaccgt cattgatcgg gagccggggg
     6001 ccgccctgga aaccagcgcc gcgaatgccg ggcaaatctc ccccggatat gctgcaccgt
     6061 gggcggcacc aggtgtgcct ttaaaagcga tcaaatggat gttccagcgc catgcgccgc
     6121 tggcggttcg tctcgacggt acgcagtttc agttgaaatg gatgtggcaa atgttacgta
     6181 actgcgacac cagccactac atggaaaaca aagggcggat ggtgcgtctg gcggaataca
     6241 gccgtgattg cctgaaagca ttgcgcgccg aaaccaatat tcagtatgaa gggcgtcagg
     6301 gtgggacgct gcaactgttc cgtaccgaac aacagtatga aaatgcgacc cgcgatatcg
     6361 ccgtgctgga agatgccggc gtaccgtatc agctgctgga atccagccgc ctggcggaag
     6421 tggagcccgc gctggcagaa gtagcgcaca aactgacggg tggtctgcaa ctgcccaatg
     6481 atgaaaccgg agactgccag ctgtttaccc agaatctggc gcggatggca gagcaggcgg
     6541 gggttaaatt ccgctttaat acgcccgttg accaactgct ttgcgacggc gagcaaatct
     6601 acggcgtgaa gtgtggcgat gaagtgatta aggccgatgc gtatgtgatg gcgtttggtt
     6661 cttactcgac ggcgatgctc aaaggcattg ttgatattcc ggtgtatccg ctgaaaggct
     6721 actcgctgac tattccaatt gcgcaggaag atggtgcgcc agtatccacc attcttgatg
     6781 aaacctacaa aatcgccatt acccgtttcg ataaccgcat tcgtgttggc ggaatggcgg
     6841 aaattgttgg ttttaacacc gagctgttgc aaccgcgtcg tgaaacgctg gaaatggtgg
     6901 ttcgcgatct ctatccacgc ggcggtcatg tcgagcaggc gactttctgg acggggttgc
     6961 gcccgatgac gccagacggt acgccggttg tcgggcgtac acgctttaaa aatctgtggc
     7021 tgaataccgg tcacggcacg ctcggctgga cgatggcttg cggttccggt cagttgttaa
     7081 gcgatctgct ctctggtcgc acgccagcga tcccatatga ggatctaagc gtagcgcgct
     7141 acagccgtgg atttacgcca tcacgtccgg gccatttaca tggcgcacac agctaaggaa
     7201 acgagatgac ccgtccgata caggccagcc tcgatctgca ggcattaaaa cagaatctgt
     7261 ccattgtccg ccaggccgcg ccgcacgcgc gcgtctggtc ggtggtaaaa gcgaacgctt
     7321 acgggcatgg tattgagcgt atctggagcg cgctcggggc caccgatggc tttgcattac
     7381 ttaacctgga agaggcaata acgttacgtg agcgcggctg gaaggggccg atcctgatgc
     7441 tggaaggatt tttccatgct caggatctgg agatttatga ccagcaccgc ctgaccacct
     7501 gcgtacacag caactggcag ctcaaagcac tgcaaaatgc gcggctaaaa gcaccgttgg
     7561 atatttatct taaagtgaac agtgggatga atcggttggg cttccagccc gatcgcgtgc
     7621 ttaccgtctg gcagcagttg cgggcaatgg cgaatgttgg cgagatgacc ctgatgtcgc
     7681 attttgccga agcggaacat cctgatggaa tttccagcgc gatggcgcgt attgagcagg
     7741 cggcggaagg gctggagtgt cggcgttcgt tgtccaattc ggcggcgact ctgtggcacc
     7801 cggaagcgca ttttgactgg gttcggcctg gcattatttt gtatggcgct tcgccgtccg
     7861 gtcagtggcg tgatatcgcc aataccggat tacgtccggt gatgacgcta agcagtgaga
     7921 ttattggtgt ccagacgcta aaagcgggtg agcgtgtggg ctacggcggt cgctatactg
     7981 cgcgcgatga acagcgaatc ggcattgtcg ccgcagggta cgccgacggt tatccgcgcc
     8041 acgcgcctac cggtacccct gttttagtgg acggcgtgcg caccatgacg gtggggaccg
     8101 tctcgatgga tatgctagcg gtcgatttaa cgccttgccc gcaggcgggt attggtacgc
     8161 cggttgagct gtggggcaag gagatcaaaa ttgatgatgt cgccgccgct gccggaacgg
     8221 tgggctatga gttgatgtgc gcgctggtgc tacgcgtccc ggttgtgacg gtgtaacttg
     8281 ttgtaagccg gatcggaggc aacgtcttct gggtgcaaaa aaaatcatcc atccggctgg
     8341 tcagcaactg tagttgttaa tgtgacagag ccattgccca tgatagtgtc cattaaaagg
     8401 atggacacta tttccccgga acctgaactc accgcacagg cgttctacat aaaacgctta
     8461 cgcttcattg ttgactcgaa ctcgacttca gataaatcac gctttaccct tgatggagcc
     8521 tgtacataga tttgtgtaat tgcctgattt tgatatgttc aattcaacat caaatgaagg
     8581 ttaaattatg gacgacaaac aattgcaggc tcaggctgcg ttcagcaaag catcgcaacc
     8641 ggcgatagat gcttcattaa atttaagatt cagcttcctc ttcagccacc cgtacgccaa
     8701 tcttcaacac ttcattatct tctttctcgg ccaccgtcca gatcatcccg gcaaactcta
     8761 cctggtcacc gacaaccggt gccgcgccta acaactgctg gacaatttca cccagcgttt
     8821 gctgcttatc acgatattct cgcccgtctt ccaggccata tatcagcgca acatcagcat
     8881 atttggcgct ggcttcgaga atgaagtcac caaagaagcg ttgatccagc gcgaccggtg
     8941 gcgactggct gaacagttta ccgagcgccg ggagatcgcg ttcccgacca attacacaca
     9001 acacatcgcc ttcgcgcagt cgggtgctgc cggtaggatg aagcaactgg ttatcacga
//
LOCUS       DAJTZL010000158         9036 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.158, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000158 DAJTZL010000000
VERSION     DAJTZL010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9036
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.158"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            142..1125
                     /locus_tag="OO544_003325"
     CDS             142..1125
                     /locus_tag="OO544_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCR6768846.1"
                     /translation="MSNDYPLVSIIIPTYNSSDYITETLTKLEKQTYPNFEIVIVNDG
                     SKDNTSNVLREYGLTHSRLIIINKENGGVSSARNTGIRKAQGQFICFMDDDDEIDPNY
                     LLKMYTRQHETGGDAIYCGLYGHYIKDGVTYSPINTEFNEGSLLFDFFYKKVRFHIGC
                     LFIRKQLLEENNLFFDEDLRLGEDLDFIYRLLITCDMYAVPYYMYKHNYRENSLMNSC
                     RTITHYRHESFAHERIYSSVMQLYKGNRKEEIHTLLSQNRAYHKTRYLWNVLLNGDFE
                     LLNQLVESNEKELNDCNLSGKRDKRRAKILASKNYILWRMVRLVNRKKNKR"
     gene            1171..2424
                     /locus_tag="OO544_003326"
     CDS             1171..2424
                     /locus_tag="OO544_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012602581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HCR6768847.1"
                     /translation="MSFCWNEINSGIKSLILILCIFSLMTLSLWDDVATKFLHAAGII
                     SALYFLITPKKTITNNPTLLIFISLCLLGIINIIWYSHYKVSGSVYTNAYRGPMETGK
                     IALCSAFIFLVLFAKNELRTKIKFGKLILFASLATQLLFFAHAMWQHFYLNVDRVALS
                     ASHATTAGYIILFPSLLASILILKSDFRHKTTLYTINFMLSLCAVIVTETRAAILVFP
                     FFALLLIVMDSYINKRINYKLYCFIAIALLAGVFSFKDTLLMRMNNLNNDLVNYSHDN
                     TRTSVGARLAMYEVGLKTYSPIGQSLEKRAEKIHELEEKEPRLSGALPYIDSHLHNDL
                     IDTLSTRGIPGVVLTILAFSAILIYALRTAKEPYILILLFSLLVVGLSDVILFSKPVP
                     TAVFVTIILLCAYFKAQSDQCLLDK"
     gene            complement(2494..3465)
                     /gene="rfaC"
                     /locus_tag="OO544_003327"
     CDS             complement(2494..3465)
                     /gene="rfaC"
                     /locus_tag="OO544_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HCR6768848.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGMDWQTAREPLASLFYNRKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQMVCRAPGNELSQLTANAVKRFIEENVAMI"
     gene            complement(3469..4515)
                     /gene="rfaF"
                     /locus_tag="OO544_003328"
     CDS             complement(3469..4515)
                     /gene="rfaF"
                     /locus_tag="OO544_003328"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HCR6768849.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDLRVLDKEAWPLMVERYVALAYDKGVMRTAQDLPKPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            complement(4525..5457)
                     /gene="rfaD"
                     /locus_tag="OO544_003329"
     CDS             complement(4525..5457)
                     /gene="rfaD"
                     /locus_tag="OO544_003329"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HCR6768850.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            5671..6867
                     /gene="kbl"
                     /locus_tag="OO544_003330"
     CDS             5671..6867
                     /gene="kbl"
                     /locus_tag="OO544_003330"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HCR6768851.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANYPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            6877..7902
                     /gene="tdh"
                     /locus_tag="OO544_003331"
     CDS             6877..7902
                     /gene="tdh"
                     /locus_tag="OO544_003331"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HCR6768852.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTVICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMAEGFDIGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            8141..>9036
                     /gene="waaH"
                     /locus_tag="OO544_003332"
     CDS             8141..>9036
                     /gene="waaH"
                     /locus_tag="OO544_003332"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HCR6768853.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWCFRETGETWKSIPSDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFNALRARYTEQSLYKYYLH
                     NTSVSRLHRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIYPEFHQQITYEALRVCH
                     AVRKEPDIITRQRMIAEIFTSG"
BASE COUNT         2395 a         2011 c         2116 g         2514 t
ORIGIN      
        1 tttatttact ttattaaatt taaattaaat aagtataaaa gaaaactatt tggataataa
       61 taaagatcat ggatgtctga gttacaataa taattaagga tgagtactat catattttat
      121 tagttcaggc aagagaacac gatgagcaat gattaccctt tagtatccat aataataccg
      181 acgtataatt catctgatta cattactgaa actctaacga aattagaaaa acaaacttac
      241 ccaaattttg aaattgttat tgttaatgat ggttctaaag ataacacatc aaacgttttg
      301 agagagtatg ggttaaccca ctctcgatta attattatca ataaagaaaa tggcggtgtt
      361 tcgtctgcca ggaatacagg tatccgcaag gcgcaaggac agtttatatg ttttatggat
      421 gatgatgatg agatagatcc taactatctg ctgaagatgt ataccagaca acatgagacg
      481 ggaggggatg cgatttattg tgggctttat ggccattata taaaagatgg tgttacttac
      541 tcacctataa atacagagtt taatgaagga tctttacttt tcgacttttt ttataaaaag
      601 gttagattcc atatagggtg cttgtttata agaaaacaac ttctggaaga gaataatctt
      661 ttttttgatg aagatttacg actaggagaa gatctggatt ttatctatcg actgctaatt
      721 acatgcgata tgtatgcggt tccatattat atgtataagc ataactatag agaaaattcc
      781 ttaatgaact catgtagaac catcactcat tatcgacatg agtcatttgc gcacgaaaga
      841 atctactctt ctgtgatgca gttatacaaa ggtaacagga aagaagaaat tcatacatta
      901 ttgagtcaaa atagagctta tcataaaact cgttatttat ggaatgttct acttaatggt
      961 gattttgaac tattaaatca attagttgaa agcaatgaaa aagaattgaa tgattgtaat
     1021 ctttctggca agagagataa aagacgagca aaaatattag catcaaaaaa ttatattctc
     1081 tggaggatgg taagactggt aaataggaaa aagaataaac gttagatctt gcattaaata
     1141 atccgtcctc gtagcatagg ttgaaactat atgtcatttt gttggaatga aattaattct
     1201 ggtataaagt ctttaattct catattatgt attttttctt tgatgacttt gtctttatgg
     1261 gatgatgttg caacaaagtt tcttcatgca gctggaatta tatctgcatt gtattttcta
     1321 ataacaccaa aaaaaacaat aactaacaat cctactttgt taattttcat ctcattatgt
     1381 cttttgggaa tcataaatat catctggtat tcacattata aagtttcagg ctctgtttat
     1441 accaatgcat atcgtggccc aatggaaact gggaaaattg ccttgtgtag tgcttttatt
     1501 ttcttggttc tttttgctaa aaatgagttg agaacaaaaa taaaatttgg gaaactaatt
     1561 ctgttcgcat ccctggcaac gcagttactt ttttttgcgc atgccatgtg gcaacatttc
     1621 tatttaaacg ttgaccgtgt tgcattatca gcttcccacg ctacaacagc aggctacatc
     1681 atccttttcc cttctttact ggcatcaatt ctcattttaa aatccgactt tagacataaa
     1741 acaacattat atacaattaa cttcatgctt agcttatgtg ctgtcatagt aactgaaacg
     1801 cgtgcagcca tattagtgtt tccattcttt gcgttactat taatcgtaat ggatagttat
     1861 attaataagc gaattaatta taagttatat tgttttattg cgattgcatt attagcaggt
     1921 gtattttctt ttaaagatac attgcttatg agaatgaata acttaaataa cgatttagtt
     1981 aattattcgc atgataacac cagaacttca gtcggtgccc gtctggcaat gtatgaagtt
     2041 ggcttaaaaa catattctcc aataggacaa tcactggaaa aacgagcgga aaaaatacat
     2101 gaactagaag aaaaagagcc tagattgagt ggcgctttac cctatataga ttctcatttg
     2161 cataacgatc tcatagatac gttatcaacg cgtggtattc ctggagttgt attaacaatt
     2221 ttagcctttt cagcaatact catatatgcc ttaagaactg ctaaagaacc ttatatttta
     2281 atcttgcttt tttcactact ggtagtaggc ctaagtgatg taatactctt ttctaaaccg
     2341 gttccgactg ctgtgtttgt caccataata ttgctttgtg cttattttaa agcacaatca
     2401 gaccaatgtt tattagataa ataatgaata tgtgaaataa aatcagcaag gaggctgatt
     2461 ttatttcaaa attatctatt aaggggttgt aatttaaatc atagcaacgt tttcttcaat
     2521 gaatcgctta acagcatttg ctgtcaattg agacaactca ttccccggag ccctacaaac
     2581 catctgattc ttcccatatc caccaattaa tcccggatcg gttggtccat aaaccgtgat
     2641 attgggtcta tccagtgccg ccgttaaatg gcttaacccc gtatccaccg acactacaaa
     2701 tttagccccg gccagcacgc gggcaacgcc ttccagactc atcttcggca atacttcaac
     2761 ataagcaaat ccttccgcca gtcgtttcgc ccgctcttcc tcatgcggcg cgccccacgg
     2821 aagtttaatc cgtattcctg aatcagccag taaaccaatc aattctcgcc agtgttcttc
     2881 cggccagtgt ttatcatcac gggtcgtcgc atgaagaaat acggcatatt cgccagcatc
     2941 tgtaggcagg ttcgtcagga aatgctgtgc aatagcataa tcgccctggg tttgcggttt
     3001 gctatagccc agacttttgg caaacagttc gcgggtgcgt tctacggcgt gctgctgttt
     3061 tgcaatatga tgcttacgat tgtaaaacag actggctaaa ggttcgcgag cggtttgcca
     3121 gtccatgcca tgctttacgc catgcgccag acgcgtcacc agcgccgcgc tttttaccag
     3181 cccctgagcg tcgataactg cgtcatagtt ctctgcttgt agcgcttcac gaaacgcttt
     3241 gcgttccgct tttatggggg ccgagaacca ggctttacgc cagcgacgta ttgccacagg
     3301 aataactcgc tcaacggcgg cgtgccagga aggaatctgt gcgaaccctt cttccaccac
     3361 ccagtcaaac ttaatccctg ggattgcctg ctgggcatca gtgagtgcgg gcaacgtatg
     3421 gagaacatcg cccatcgacg atgttttaac gatcaaaacc cgcatccgtc aggcttcctc
     3481 ttgtaacaat agcgcgttga gttcttccag tacgcgctgg ggagtaatgt cgattaagct
     3541 ctggtgataa ccctccgcag cgtcaccttt acgcactttg tgatagccgg taatcaggcg
     3601 gatcacgcgc gctttatggg atagcggcgg tgtgaagtcc gggctactcg gaccatacag
     3661 ggcaaccagc ggacgattga gcgccgccgc aacgtgcatc aggccagaat cgttagtgac
     3721 aatggcttta caggctgcaa tcaggataac cgcttgatca agctgtgttt cccccgccag
     3781 gttccgacac catgcctgct gctcggtatt caaagcggca agaatctcat tgcccgcttc
     3841 atgatctttc gccgagccaa acagaaccac ctgataacct tcatcaatca gctgctttgc
     3901 cagttccgca tagtggtagt gtggccagcg ttttgccgga ccaaactccg cacccgggca
     3961 aaaaccaatc atcggacgtt ctgatgaaag cgaaaattga ttacaggtat atgatttttc
     4021 accttcgctc acctgcaatt gcggccataa caacggtttt ggtagatctt gtgcggtacg
     4081 catcacgcct ttgtcatagg ccagcgcgac atagcgttcc accattagcg gccaggcttc
     4141 tttatcgagc acgcgtaaat cgttgagtaa accgtagcgc atctcgccgc gccagccggt
     4201 gcgatgagga atacccgcga agaaaggcac taatgcagat ttgaaggagt tgggtaagac
     4261 gtaggcgcgg tcgtagcgct tttcacgcag gctatgaccc agtttgcggc gttcgccgat
     4321 ttccagcgct ccgtgaccga gaggcatagg aatagcttcg ttaacttccg gcatccgcga
     4381 taataatgga cggcaccatg ccggtgccat cacatcgatt atcgcctggg gatagcgcgc
     4441 ctggagcgtg cgatagagac tttgcgacat catcatgtcg ccaacccaag acgggccgat
     4501 caccagtatt ttcatgcaga gctcttatgc gtcgcgattc agccaggcca tgtattccgt
     4561 tacaccttca gcaacggttt tgaacggttt gtcgtaaccc gccgcgcgca gatttgtcag
     4621 atctgcctga gtgaacgcct ggtagcggcc tttcagctta tccgggaacg gaatgtattc
     4681 gatctggcct ttcttgtgat aagccagcgt agcatcagct acagcctgga aggattccgc
     4741 acgaccagta ccgaggttga agatgccgga aacgccattt tccaggaacc acagattcac
     4801 atcagccacg tcgcccacat agacgaagtc gcgtttgaag ttctcgctac cttcaaacag
     4861 cttcggtgat tcaccgttgt taagctgggt gttgagatgg aaagcgacgc tcgccatgct
     4921 gcctttatgg ccttcacgcg gtccataaac gttgaaatag cggaagccaa caatctgcga
     4981 gttagcttca ggcaggattt ggcgaacata ttcatcaaac aggaattttg agtaaccgta
     5041 gacgttcaac ggtttttcgt actcgcggga ttcaataaag tcggaggtgc gtccgccgta
     5101 ggtggctgcg gaagaggcat acaggaacgg gatttcgcgc tccagacagt agtgcagcag
     5161 ctctttggag tattgatagt tgttatccat catatacttg ccatcccact cggtggtgga
     5221 agagcacgcg ccttcgtgga aaatcgcttc gacatcgccg aactcttcgc cagccataat
     5281 ctggatcagg aagtcttcct tatccatata gtccgcgata tccagatcca ccaggttcac
     5341 aaacttggtg ccgtctttca ggttgtccac caccagaata tcggtgatgc ctttatcatt
     5401 cagggcctta acgatgttgc tgccgataaa gcccgcgccg ccggtaacga tgatcataac
     5461 tgtaaccttc gaattatgga gtcagagaca atctcagaca cgaatacttc tatcatatca
     5521 ctacacggcg ttggcttcag ccattcgcca aaagtgagat tgatggacgt tatttatcct
     5581 gaattttgca gaagtgttaa cgcgtcatct cgtcgcgacc tataagtttg ggtaatatgt
     5641 gctggaattt gccctgtctg gagaatcgca atgcgtggag aattttatca gcagttaacc
     5701 aacgatctgg aaaccgcacg ggcggaaggg ttgtttaaag aagagcgcat tattacgtct
     5761 gcgcagcaag cagatatcac tgtggctgat ggaagccacg tcattaactt ttgtgccaat
     5821 aactatctcg ggctggcgaa ttatccggat ctgattgcgg cggcaaaggc gggaatggat
     5881 tctcacggtt tcggcatggc ttcggtgcgt tttatttgcg gcactcagga cagccataaa
     5941 gagcttgaac aaaaactggc ggcattcctg gggatggaag atgcgattct ctactcttcc
     6001 tgctttgatg ctaacggtgg cctgtttgaa acgctgctgg gcgcggaaga cgccattatc
     6061 tccgacgcac tgaaccacgc gtctattatt gatggtgtgc gtctgtgtaa agctaaacgc
     6121 tatcgctatg ccaacaacga tatgcaggag ctggaagcac gtctgaaaga agcgcgtgaa
     6181 gccggtgcgc gtcatgtgct gattgccacc gatggtgtgt tctcgatgga cggcgtgatt
     6241 gccaacctga agggcgtttg cgatctcgca gataaatatg atgccctggt gatggtagac
     6301 gactcccacg cggtcggttt tgtcggtgaa aatggtcgtg gttcccatga atactgcgat
     6361 gtgatgggcc gggtcgatat tatcaccggc acgctcggta aagcgctggg cggggcttct
     6421 ggtggttata ccgcggcgcg caaagaagtg gttgagtggc tgcgccagcg ttctcgtccg
     6481 tacctgtttt ccaactcgct ggcaccggcc attgttgccg catccatcaa agtactggag
     6541 atggtcgaag cgggtagcga gctgcgtgac cgtctgtggg cgaacgcgcg tcagttccgt
     6601 gagcaaatgt cggcggcggg ctttaccctg gcgggagccg atcacgccat tattccggtc
     6661 atgcttggtg atgcggtagt ggcgcagaaa tttgcccgtg agctgcaaaa agaaggcatt
     6721 tacgtcaccg gtttcttcta tccggtcgtt ccgaaaggtc aggcgcgtat tcgtacccag
     6781 atgtctgcgg cgcatacccc tgagcaaatt acgcgtgcag tagaagcatt cacgcgtatt
     6841 ggtaaacaac tgggcgttat tgcctgagga tgtgagatga aagcgttatc caaactgaaa
     6901 gcggaagagg gcatctggat gaccgacgtt cctgtaccgg aactcgggca taacgatctg
     6961 ctgattaaaa tccgtaaaac agtcatctgc gggactgacg ttcacatcta taactgggat
     7021 gagtggtcgc aaaaaaccat cccggtgccg atggtcgtgg gccatgaata tgtcggtgaa
     7081 gtggtaggta ttggtcagga agtgaagggc ttcaagattg gcgatcgcgt ttctggcgaa
     7141 ggccatatca cctgcggtca ctgtcgcaac tgtcgtggcg gtcgtaccca tctgtgccgc
     7201 aacacgatcg gcgtcggcgt taaccgcccg ggctgcttcg ccgaatatct ggtgattccg
     7261 gcgttcaacg ccttcaaaat tcccgacaat atctccgacg acctggcttc catttttgat
     7321 cccttcggta acgccgtgca taccgcgctg tcgttcgatc tggttggcga ggatgtgctg
     7381 gtctctggtg caggcccgat tggtattatg gcagcggcgg tggcgaaaca cgttggtgca
     7441 cgcaatgtgg tgatcactga tgttaacgaa tatcgtctcg aactggcgcg caaaatgggt
     7501 atcacccgtg cggtcaacgt cgctaaagaa aaccttaatg atgtgatggc agaactgggc
     7561 atggccgaag gctttgatat cggtctggaa atgtccggtg cgccgccagc gtttcgtacc
     7621 atgctcgaca ccatgaacca cggcggtcgc attgcgatgc tgggtattcc accgtccgat
     7681 atgtctatcg actggaccaa agtaatcttt aaaggcttgt tcattaaagg tatttacggt
     7741 cgtgagatgt ttgaaacctg gtacaagatg gcggcgctga ttcagtcagg tctggatctc
     7801 tcgccgatca ttacccatcg tttctctatc gatgatttcc agaagggctt tgacgctatg
     7861 cgttcgggcc agtccgggaa agtaattctg agctgggatt aacacgaaca agggctggta
     7921 ttccagccct tttgcctgag gataatccgt taaatatgta aaatcctgtc agtgtaataa
     7981 agatatcgta attgcgctga tctcttatat agctgctctc attatctctc taccctgaag
     8041 tgactctctc acctgtaaaa ataatatctc acaggcttaa tagtttctta atacaaagcc
     8101 tgtaaaacgt caggataact tcagaggtcg tcggtaattt atgatgaaca gcaccaataa
     8161 acttagtgtt attattccgt tatataatgc gggcgatgat ttccgcactt gtatggaatc
     8221 tttaattacg caaacctgga ctgctctgga aatcattatt attaacgatg gttcaacgga
     8281 taattctgtt gaaatagcaa agcattacgc agaaaactat ccgcacgttc gtttgttgca
     8341 tcaggcgaat gctggcgcat cggtggcgcg taatcgtggg attgaagtgg caacgggcaa
     8401 atatgtcgct tttgtcgatg ctgacgatga agtctatccc accatgtacg aaacgctgat
     8461 gaccatggcg ttagaggacg acctcgacgt ggcgcagtgc aacgctgact ggtgttttcg
     8521 tgaaacggga gaaacctgga aatccatccc cagcgatcgc cttcgctcaa ccggcgtatt
     8581 aaccggcccg gactggctgc ggatggggct ttcttcgcgc cgttggactc acgttgtctg
     8641 gatgggggtt tatcgccgcg atgttattgt taaaaataac attaaattta ttgccggatt
     8701 acatcatcag gatattgtct ggacaacaga atttatgttt aatgcgctgc gtgcgcgata
     8761 taccgagcaa tcgttatata aatattatct gcataatacg tcagtgagtc ggttgcatag
     8821 acaagggaac aaaaacctta attatcaacg tcactatatt aagattactc gtctgctaga
     8881 gaaattaaat cgaaattatg ccgacaaaat tacgatttat ccggaatttc atcagcaaat
     8941 aacttacgaa gcattgcgtg tttgccatgc ggtgcgcaaa gagccggata ttattacccg
     9001 ccaacggatg attgccgaga tatttacttc cggtat
//
LOCUS       DAJTZL010000159         8912 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.159, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000159 DAJTZL010000000
VERSION     DAJTZL010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8912
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.159"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(38..400)
                     /locus_tag="OO544_003333"
     CDS             complement(38..400)
                     /locus_tag="OO544_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HCR6768854.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATDPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            937..2850
                     /gene="mtlA"
                     /locus_tag="OO544_003334"
     CDS             937..2850
                     /gene="mtlA"
                     /locus_tag="OO544_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HCR6768855.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            3080..4228
                     /gene="mtlD"
                     /locus_tag="OO544_003335"
     CDS             3080..4228
                     /gene="mtlD"
                     /locus_tag="OO544_003335"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCR6768856.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISDLDANSEVVSEAVTAYKAMQ"
     gene            4228..4815
                     /gene="mtlR"
                     /locus_tag="OO544_003336"
     CDS             4228..4815
                     /gene="mtlR"
                     /locus_tag="OO544_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HCR6768857.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISRISLKKAFQK"
     gene            complement(4827..5036)
                     /locus_tag="OO544_003337"
     CDS             complement(4827..5036)
                     /locus_tag="OO544_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6768858.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            5321..5683
                     /locus_tag="OO544_003338"
     CDS             5321..5683
                     /locus_tag="OO544_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HCR6768859.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLQEMTGFSKTAF"
     gene            6226..6904
                     /locus_tag="OO544_003339"
                     /pseudo
     CDS             6226..6904
                     /locus_tag="OO544_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
     gene            6948..>8912
                     /locus_tag="OO544_003340"
     CDS             6948..>8912
                     /locus_tag="OO544_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HCR6768860.1"
                     /translation="MNKIFKVIWNPATGNYTVTSETAKSRGKKSGRSKLLISALVAGG
                     MLSSFGALANAGNDNGQGVDYGSGSAGDSWVAIGKGAKANTFMNTSGSSTAVGYDAIA
                     EGQYSSAIGSKTHAIGGASMAFGVSAISEGDRSIALGASSYSLGQYSMALGRYSKALG
                     KLSIAMGDSSKAEGANAIALGNATKATEIMSIALGDTANASKAYSMALGASSVASEEN
                     AIALGRSSVASGTDSLAFGRQSLASAANAIAIGAETEAAENATAIGNNAKAKGTNSMA
                     MGFGSLADKVNTIALGNGSQALADNAIAIGQGNKADGVDAIALGNGSQSRGLNTIALG
                     TASNATGDKSLALGSNSSANGINSVALGADSIADLDNTVSVGNSSLKRKIVNVKNGAI
                     KSDSYDAINGSQLYAISDSVAKRLGGGAAVDVDDGTVTAPTYNLKNGSKNNVGAALAV
                     LDENTLQWDQTKGKYSAAHGTSSPTASVITDVADGTISASSKDAVNGSQLKATNDDVE
                     ANTANIATNTSNIATNTASIATNTTNITNLTDSVGDLQADALLWNETKKAFSAAHGQD
                     TTSKITNVKDADLTADSTDAVNGSQLKTTNDAVATNTTNIANNTSNIATNTTNISNLT
                     ETVTNLGEDALKWDKDNGVFTAAHGTETTSK"
BASE COUNT         2445 a         2100 c         2204 g         2163 t
ORIGIN      
        1 agcaccacgt aagttaaaac aatcaataga tccataatta gatctccggg aacgtggaat
       61 agtcagtctt tttctcggcg gcgagttgat gctctagccg ggaaattcgg tcagaaagtg
      121 cggcgatttc cgggtcagtc gctttctcct gcgacgcaac atgtgactgc atgccccacc
      181 cgcgctccgg ttgatagagc gtcgcccaga tccacaggaa cggccagata acatgcaggg
      241 taaacaggct cacccagccc gccgtatgaa tagcgtcggc atggggatgg ttgcgctttt
      301 tggcaatcag ataagggatg tcatgtatcg cgatgattcc ataaaaaatc accagaaaga
      361 cgaagatcag cactcccaac gcgaaatagt ttagaaacat atctgcctcg gattcacgtt
      421 tatcagtgtt gtttttgggc tggcagccag aagggagtca ggctgatatt ttgacaataa
      481 tccgggttcg cgattctcgc cataacacca aagaataatt tttagaggtg atgagttgct
      541 tagttacata acaattgtat gacgaaggca taacatgctg tagatcacat caggtgaacg
      601 ccgtaagaaa atatcttgtg attcagatca caaagattca acaaaccatc aaaacaaaaa
      661 tgtgacacta ctcacattta aatgccattt ttagcgaaaa tcgccgcctt gttgcttttt
      721 tacacaagcg ttttgtgatg aacgtcacgt taattacctc tctaccccct atatttatgt
      781 gattgatatc acacaaaagg ccgtcgactg gacagttaac cgattcagtg ccagatttcg
      841 cagtatctac aaggtccggc tacctctgcc gccacattaa caaaaaacct cgggcttcca
      901 gcctgcgcga cagcaaacat aagaaggggt gtttttatgt catccgatat taagatcaaa
      961 gtgcaaagct ttggtcgttt cctcagcaac atggtgatgc caaatatcgg cgcgtttatc
     1021 gcgtggggta tcatcaccgc gttatttatt ccaacagggt ggttaccgaa cgagacgctg
     1081 gcgaagctgg tcgggccgat gatcacttat ctcctgccgc tgctgatcgg ttataccggt
     1141 ggtaagctgg taggcggcga acgtggcggc gtagtcggtg ccatcaccac catgggcgtt
     1201 atcgtcggcg cagacatgcc gatgttcctc ggttctatga ttgcaggtcc gctgggcggc
     1261 tggtgcatta agcacttcga ccgctgggta gacggtaaga tcaaatccgg ttttgagatg
     1321 ctggtgaata acttctccgc aggcatcatc gggatgatcc tcgctattct ggcattcctc
     1381 ggcattggcc cgattgttga agccctgtcc aaaatgctgg ctgcgggcgt taacttcatg
     1441 gttgtccatg acatgctgcc gctggcgtct atctttgttg aaccggcgaa aatcctgttc
     1501 ctcaacaacg ccattaacca cggtatcttc tcgccgctgg gtattcagca gtcccatgaa
     1561 ctgggtaaat caatcttctt cctgattgaa gctaacccag gtccaggtat gggcgtgctg
     1621 ctggcgtaca tgttctttgg tcgtggtagc gctaaacagt ctgcgggcgg tgcggcaatc
     1681 atccacttcc tgggtggtat ccacgaaatc tacttcccgt atgtgctgat gaatccgcgt
     1741 ctgatcctcg cagtcatcct cggcggtatg actggcgtgt tcacgctgac tatcctgggc
     1801 ggtggtctgg tttctccggc atctccgggt tctatccttg ctgtactggc gatgacacca
     1861 aaaggtgctt atttcgctaa catcgcgggt gtgtgtgcgg cgatggctgt ctccttcgtt
     1921 gtctctgcta ttttgctgaa aaccagcaaa gtgaaagaag aagatgatat tgaagcagca
     1981 actcgtcgta tgcaggacat gaaagctgag tctaaaggca catctccgct gtctgcgggc
     2041 gatgtgacta acgacctgag ccacgtacgt aaaatcatcg ttgcctgtga cgccggtatg
     2101 ggttccagtg cgatgggcgc aggcgtgctg cgtaagaaaa ttcaggatgc aggtctgtca
     2161 cagatttcgg tcactaacag cgcgatcaac aacctgccgc cagatgtgga cctcgtcatc
     2221 actcaccgtg acctgaccga acgcgctatg cgccaggttc cgcaggcgca gcatatttcg
     2281 ctgaccaact tcctcgacag cggcctgtac accagcctaa ccgaacgtct ggttgctgcc
     2341 cagcgccata ctgaaaacga agtgaaagtg aaagacagtc tgaaagacag ctttgacgat
     2401 tccagtgcta acctgttcaa actgggcgcg gagaacatct tcctcggtcg caaagcagca
     2461 accaaagaag aagcgattcg ttttgctggc gagcagctgg tgaaaggcgg ttacgttgag
     2521 ccggaatacg ttcaggcgat gctggatcgt gaaaaactga ccccgactta tctgggtgag
     2581 tctatcgcgg tgccacacgg tacggttgaa gcgaaagatc gcgtactgaa aacgggcgtg
     2641 gtgttctgcc agtacccgga aggcgtgcgc ttcggtgaag aagaagatga cattgcccgt
     2701 ctggtgattg gtattgcagc ccgtaacaac gagcacattc aggttatcac cagcctgacc
     2761 aatgcactgg atgatgagtc cgtcatcgag cgtctggcac acaccaccag cgtggatgaa
     2821 gtgctggaac tgttggcagg tcgtaagtaa tccaatccca ccctctccac atggagaagg
     2881 tggggttaat tgcctgatgc gctacgctta tcaggcctac aggatgcatc acgatttgtt
     2941 gaatttgcac gttcttgtag gccggataag gcgcttacgc cgcatccggc gctgcccctc
     3001 tcctcacgga gagggtttgg gtgagggaaa agcctcaccc cagccctctc gggtaaaaac
     3061 attgatgaag gttaatacta tgaaagcatt acattttggc gcaggtaata tcggtcgtgg
     3121 ctttatcggt aaactgctgg cagacgcggg tatccaactg acgtttgccg atgtcaatca
     3181 ggtggtactt gatgccctga atgcccgtca tagctatcag gttcatgtgg ttggtgaaac
     3241 cgagcaggta gataccgttt ccggcgtcaa tgctgtcagc agcattggtg atgatgtcgt
     3301 tgatctgatt gctcaggtcg atttagtcac taccgccgtt ggcccggttg tgctggaacg
     3361 tattgctccg gctatcgcca aagggctggt gaaacgcaaa gaacaaggta atgaatcccc
     3421 gctgaacatc atcgcctgtg aaaacatggt acgcggtacc acgcagctga aaggccatgt
     3481 gatgaacgcc ctgccggaag acgccaaagc gtgggtagaa gaacacgttg gctttgtcga
     3541 ttccgccgtt gaccgcatcg taccgccttc ggcttcggca actaacgatc cgctggaagt
     3601 gacggtagaa actttcagcg aatggattgt cgataaaacg cagttcaaag gcgcactgcc
     3661 gaacatccca ggcatggagt taaccgacaa cctgatggca tttgtcgaac gtaaactctt
     3721 caccctgaac acgggtcatg ctataaccgc gtacctcgga aaactggccg gtcatcagac
     3781 cattcgtgac gctattctcg acgagaaaat ccgcgcggtg gtaaaaggtg cgatggaaga
     3841 aagtggtgcg gtactgatca agcgctacgg ctttgacgca gacaaacatg cggcgtacat
     3901 ccagaaaatc ctcggtcgtt ttgagaaccc gtatctgaaa gatgatgtag agcgcgtagg
     3961 ccgtcagccg ctgcgtaaac tgagtgctgg cgaccgtctg atcaagccat tgctcggtac
     4021 gctggaatac agccttccgc acaagaatct gattcagggg attgctggtg caatgcactt
     4081 ccgcagtgaa gatgatccgc aggctcagga actggcagca ctgatcgctg acaaaggtcc
     4141 gcaggcggcg ctggcacaga tttccgatct tgatgccaac agcgaggttg tatccgaggc
     4201 ggtaaccgct tataaagcaa tgcaataatg gtggaccagg cgcaggacac cctgcgcccg
     4261 aataacagat tgtcagatat gcaggcaaca atggaacaaa cccaggcctt tgaaaaccgt
     4321 gtgcttgagc gtctgaatgc tggcaaaacc gtgcgaagct ttctgatcac cgccgtcgag
     4381 cttttgaccg aggcggtaaa tcttctggtg cttcaggtat tccgcaaaga cgattacgcg
     4441 gtgaagtatg ctgtagaacc gttactcgac ggcgacggtc cgctgggcga tctttctgtg
     4501 cgtttaaaac tcatttacgg gttgggcgtc attaaccgcc aggaatacga agatgcggag
     4561 ctgctgatgg cattgcgtga agaactaaat cacgacggca acgagtacgc ctttaccgac
     4621 gacgagatcc ttggaccctt tggtgaactg cactgcgtgg cggcgttacc accgccgcca
     4681 cagtttgaac cagcagactc cagtttgtat gcaatgcaaa ttcagcgcta tcaacaggct
     4741 gtgcgatcaa caatggtcct ttcactgact gagctgattt ccagaatcag cttaaaaaaa
     4801 gcctttcaaa agtaagcaac gtctgcttac tgcccctcta cctgcttcgg ccgataaagc
     4861 cgacgataat actccagacg ttgtagatat aacggcacgc tttcatcggg tattccggac
     4921 ggaatcgcgt tacggggagg aagttttttc agatactccc ggaacgcctg gcttgatgcc
     4981 atgaaatcta cggctttatc gataagaagc ggaacgtttt cacctaattt gcccatgatg
     5041 atatctccgt attaccccgc gccgggaatg cgcggccgcc aattttagtt taggttcccg
     5101 gtaaagatga cattaaggaa aacgtgctga atcctcaata atcatccgct gctgtttatt
     5161 atcttttttc tatagatttc ttaattaatc aataaattgc gtagttttta agcatgttaa
     5221 tgattacgtt acagaactat tgcaatgtca tcagttgtga acaaagcacc tggtcgcgca
     5281 tactaggggc tataaattta tctttatcag aagccatcac atgaaagaag tcgaaaaaaa
     5341 cgaaatcaaa cgtctcagcg atcgtctgga cgccatccgc caccagcagg ccgatctgtc
     5401 gctggttgaa gccgcagaca aatatgccga gctggaaaaa gagaaagcca cgctggaagc
     5461 agaaattgct cgcctgcgtg aagttcatag ccaaaaactg agtaaagaag cacagaagct
     5521 gatgaagatg ccgtttcagc gcgcaattac caaaaaagag caggctgata tgggcaagct
     5581 gaagaaaagt gttcgcggac tggtggttgt gcacccaatg accgcgctgg gccgtgaaat
     5641 gggcctgcag gagatgactg gattctctaa gactgcgttt taagcgcctc agacgactgg
     5701 ttcgatcata tcaacccgca tctggcttgc cttagctggt caatgcgggg actctcctgt
     5761 ctactcgcct aattaccaaa caattcactc cctttacgtc gatttaccgc tggttgatat
     5821 tctgaaaatg catttttacc gaaatagcca cctataaaca ataaaaatat atagaagata
     5881 tcatttatta attaacaagc agttacgtct ctttttttcg gatttaattg tgaaatttta
     5941 atcatcatat tcatacaata aattatctgt aaattttttc ccagacatgg tttctttcat
     6001 atttgacatt tattaataat catcattaga atccacgaaa ataatatatt catacaaaca
     6061 cagacatctt ttgaaacgaa tgatacattt ttatgatgaa tgcttaaaac aatatttaat
     6121 cgacacacat cactgtcatt aatgtaaagc aacatagatc accaataata caatggacaa
     6181 ctctaccggc attgatcgct ggtcggagga aataaggatt tttacatgaa cttgaagaag
     6241 atttttttca gtgctgtcac tgtttcagtt ctctgcgcat taacgggatg tgactacatt
     6301 gaagaaggca agccagaaag tagtttattg aaacaacaag aagaacatta aaattgatct
     6361 acttgaaaaa caacaagcac agctaaagag tcaactggaa actattcaaa aacaacaaac
     6421 tggcataata agcagtacaa agactttaac tcacgtaatt aaatccgtta aagatcaaca
     6481 aaacactttt atttttaccg agtttaaccc agcaaaaacg aagtatttca tcctgaacaa
     6541 tggttcggtg gctttagcgg gtcgagtgtt atccattgac gccacagaga atggcagtgt
     6601 tattcatatt tcactggtca acttattaag tacacctatc tcaaatattg gttttaatgc
     6661 gacatggggt ggtgaaaaac ctgtcgatgc caaagagttt gcccggtggc aacaattact
     6721 tttcaacaca tcaatgaaat ccacattgaa attattacca ggtcaatggc aagacattaa
     6781 tttgaccctg aagggtgtat cgcccaataa cctgggatat ctgaagttag ccatcaacat
     6841 ggaaaatatt cagttcgaca atcttccctc tgctgaaaat cggcagaaaa gaagcaaaaa
     6901 ataaatgcag ttagtacgct tataacaagg aatagcgagc atcattaatg aacaaaatat
     6961 ttaaagttat ctggaaccct gcgacaggga attatactgt taccagcgaa acggcaaaaa
     7021 gccgtggcaa gaaatctggg cgcagtaagc tgttaatttc tgcgctggtt gcgggtggaa
     7081 tgttgtcgtc gtttggggca ttggcgaatg ccgggaatga caacggccag ggtgttgatt
     7141 acggtagtgg atcagctggc gacagctggg ttgctatagg caaaggggcg aaagcaaata
     7201 cttttatgaa caccagtggt tccagtactg ctgtgggtta tgacgctata gctgaaggcc
     7261 aatatagctc tgccatcggg tcaaaaaccc atgcgattgg tggtgcatca atggcctttg
     7321 gggttagtgc aatatcagaa ggcgatagaa gtatagcact gggtgcctct tcgtattcat
     7381 tgggccaata ctcaatggcc ctcggccgtt attcaaaagc attgggtaaa ttgtctattg
     7441 ctatggggga ctcttccaaa gcggaaggag caaacgccat tgccctggga aatgccacta
     7501 aagctactga gattatgagt attgctcttg gcgacaccgc caatgcgtca aaagcgtatt
     7561 caatggcgct gggagcaagt agcgtcgcat ctgaagaaaa cgcaattgcc ctggggcgta
     7621 gcagtgtagc tagcggtact gacagcctcg catttggcag acaatcactt gccagcgcag
     7681 cgaacgctat tgcgataggt gctgagaccg aagccgctga aaatgcaact gctattggca
     7741 ataatgcgaa ggcaaaaggg actaatagca tggcaatggg gttcggaagc cttgccgata
     7801 aagtcaatac tatcgcatta ggaaatggca gccaggctct ggcagataat gcaatcgcca
     7861 taggccaggg caacaaagct gatggcgtgg atgccatcgc tctgggtaat ggtagccagt
     7921 cgagaggctt aaacaccatt gccttaggca cagccagtaa tgcaactggt gataagagtc
     7981 ttgcgcttgg tagtaatagc agtgccaacg gtattaactc tgtcgcgctg ggcgcagatt
     8041 ccattgcgga tttagacaat accgtctctg tcggcaatag ttcattaaaa cgcaagatcg
     8101 ttaatgtgaa aaatggcgcg atcaagtctg acagttacga tgccattaat ggttcacagc
     8161 tttatgccat tagcgactcg gtagcaaaaa ggcttggagg aggggctgca gtagatgttg
     8221 atgacggtac tgttacagca ccaacctaca atttaaaaaa tggtagcaaa aataacgtag
     8281 gggctgcgct cgctgtactt gatgaaaaca ccctgcaatg ggaccaaacc aaaggcaaat
     8341 acagcgctgc tcatggtact agtagcccaa ctgccagcgt aatcaccgat gttgcggatg
     8401 gcacgatttc agcctccagt aaggatgcgg ttaacggttc ccaactgaaa gctaccaatg
     8461 acgatgtcga agccaacacc gccaatatcg ctactaatac cagcaacatt gccacgaata
     8521 cagcaagtat tgccaccaat accaccaata tcaccaacct gacggattcc gttggtgacc
     8581 ttcaggctga tgccctgctc tggaacgaaa ctaaaaaggc attcagtgca gctcacggcc
     8641 aggataccac cagcaaaatc accaacgtta aagatgccga cctgacggct gacagcactg
     8701 atgctgttaa cggctctcag ctgaaaacca ccaacgatgc tgtggcgacg aataccacca
     8761 atatcgccaa taacacttcc aatattgcca ctaacaccac caacatctct aatctgactg
     8821 agacggtgac taatcttggt gaggatgcgc tgaaatggga taaggacaat ggtgtattca
     8881 cggcagctca tggcaccgag accaccagca aa
//
LOCUS       DAJTZL010000160         8872 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.160, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000160 DAJTZL010000000
VERSION     DAJTZL010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8872
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.160"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(56..1096)
                     /gene="ompA"
                     /locus_tag="OO544_003341"
     CDS             complement(56..1096)
                     /gene="ompA"
                     /locus_tag="OO544_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HCR6768861.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     INNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGSVENGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKSNVYGKNHDTGVSPVFAGGVEYAITPEIATRL
                     EYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKHFTLK
                     SDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLSER
                     RAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEV
                     KGIKDVVTQPQA"
     gene            complement(1451..1960)
                     /gene="sulA"
                     /locus_tag="OO544_003342"
     CDS             complement(1451..1960)
                     /gene="sulA"
                     /locus_tag="OO544_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor SulA"
                     /protein_id="HCR6768862.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
     gene            2179..2808
                     /gene="sxy"
                     /locus_tag="OO544_003343"
     CDS             2179..2808
                     /gene="sxy"
                     /locus_tag="OO544_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HCR6768863.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(2771..4933)
                     /gene="yccS"
                     /locus_tag="OO544_003344"
     CDS             complement(2771..4933)
                     /gene="yccS"
                     /locus_tag="OO544_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HCR6768864.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGLLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWQFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNEALASLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(4943..5389)
                     /locus_tag="OO544_003345"
     CDS             complement(4943..5389)
                     /locus_tag="OO544_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HCR6768865.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            5512..7566
                     /gene="helD"
                     /locus_tag="OO544_003346"
     CDS             5512..7566
                     /gene="helD"
                     /locus_tag="OO544_003346"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HCR6768866.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGERCDLDTTYRFNSRI
                     GKVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(7598..8056)
                     /gene="mgsA"
                     /locus_tag="OO544_003347"
     CDS             complement(7598..8056)
                     /gene="mgsA"
                     /locus_tag="OO544_003347"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HCR6768867.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(8152..8814)
                     /locus_tag="OO544_003348"
     CDS             complement(8152..8814)
                     /locus_tag="OO544_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HCR6768868.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
BASE COUNT         2313 a         2157 c         2320 g         2082 t
ORIGIN      
        1 ttactaaagg caaaaaaaac cccgcagcag cggggttttt ctaccagacg agaacttaag
       61 cctgcggctg agttacaacg tctttgatac ctttaacttc gatctctacg cgacgatccg
      121 gagccaggca gtcgatcagt gcagcacgct gtttcacgtt gtcacaggtg ttgccagtaa
      181 ccgggttgga ttcgcccata ccacgtgcgg agatcttgtc tgccgggata cctttggaga
      241 tcaggtaatc aacaacagac tgagcacggc gctcggacag accctggttg taagcgtcag
      301 aaccgatgcg gtcggtgtaa cccagaacaa ctacggaacc gtctttcgga tccaggttgc
      361 tcagctggct gtacagctga tccagagcag cctgaccttc cggtttcagg gttgctttgt
      421 tgaagttgaa cagaacgtca gacttcagag tgaagtgctt ggtctgtact tccggtgccg
      481 gagctggagc cggagcaact actggagctg cttcgccctg accgaaacgg taggaaaccc
      541 ccaggctcag catgccgttg tccggacgag tgccgatggt gtgtgcgtca ccgatgttgt
      601 tggtccactg gtattccaga cgggtagcga tttcaggagt gatcgcgtac tcaacaccgc
      661 cagcgaagac cggagaaacg ccggtgtcgt ggtttttacc ataaacgttg gatttagtgt
      721 ctgcacgcca taccatacca cccagacgag tgtagatgtc caggtcgtca gtgattgggt
      781 aacccagttt agcggtcagt tgaacgccct gagctttgta tgcaccgttt tcaacgctgc
      841 ctttgtacgg catacgacct aaccagtcgt aacccatttc aaagccaaca tacgggttaa
      901 cctggtaacc accaaaagca ccagcgccca gttggttttc atgggtcggg ccattgttgt
      961 tgatgaaacc agtgtcatgg tactgggacc agcccagttt agcaccagtg taccaggtgt
     1021 tatctttcgg agcggcctgc gctacggtag cgaaaccagc cagtgccact gcaatcgcga
     1081 tagctgtctt tttcattttt tgcgcctcgt tatcatccaa aatacgccat gaatatctcc
     1141 aacgagataa cacggttaaa tccttcaccg ggggatctgc tcaattaact ctaccgatat
     1201 cttcggctta tgccgagcac ccctggcgat gtaaagtcta caacgtagtt gaaaacttac
     1261 aagtgtgaac tccgtcaggc atatgaaaaa aaagtcttgt ataaggtatg tttaatcttt
     1321 tttgtcagcg acaatttaca gaagaaaatc gcggaaaccg cttcagacaa gcctccgcaa
     1381 ggaaaattag tcacgactga aagcattggc tgggcgacaa aaaaagttcc aggattaatc
     1441 ctaaatttac ttaatgatac aaattagagt gaatttttag cccggaaagt tgtctcgtgg
     1501 cgtgagagga tgcgcttacc ggacgcataa taaaccccat agcgttacct tcatttgccg
     1561 catcaacaag ttcagcatgc tcttcttcag tcaaatcatc tgccaaccaa ccgatcacca
     1621 cactgtaatt gcccgtgcgt aaagcgcgaa ccattgactc cacagtgtgg caaggggaga
     1681 gctggctaat ctgcattact ttcgttaagg gtagcccaga tgcctgaacc cattcccgac
     1741 tcagtttttg ttgcggtgtt aaccagagtt gccagcgcga ttgctgaccg agttgctgta
     1801 acaatggcaa cagtagaagt tgcgtcatca tgggctgatc ttcgcgatag acaacttcac
     1861 tgataagccc ggctgtagtg ttttccgtag agacacgcgc aattttactt gctgcggatg
     1921 agaacgacga agaacgatgt gcatagcctg aagtgtacat aatcaatcca gcccctatga
     1981 gttactgtat ggatgtacag tacatccagt gacaacaaag atcaacccta ttttcggaaa
     2041 gagcctcgca aattttgtcg ttggtgacgg gaaaacataa attaatcttg ccccttaaga
     2101 ataagttgcc tattttcgta gttaacggat ccgttaatgt gaatcattct tttatgttat
     2161 gattttaaaa ggaattttat gaaaagcctc tcctataagc ggatctataa atcacaagaa
     2221 tacctggcaa cgttgggcac aattgaatac cgatcattgt ttggcagtta cagcctgacc
     2281 gttgacgaca cggtgtttgc gatggtttct gatggtgagt tgtatcttcg ggcttgtgag
     2341 caaagtgcac agtactgtgt aaaacatccg cctgtctggc tgacatataa aaagtgtggc
     2401 cgatccgtta ccctcaatta ctatcgggtt gatgaaagtc tatggcgaaa tcaactgaag
     2461 ctggtgcgtc tgtcgaaata ttctctcgat gcagcgctga aagagaaaag cacgcgcaat
     2521 acccgggaaa gactgaaaga tttgcccaat atgtcttttc atctggaagc gattctcggg
     2581 gaggtgggga ttaaggatgt acgggcgtta cgtatacttg gggcaaaaat gtgttggttg
     2641 cgactgcggc agcaaaacag tctggtgaca gaaaagattc tgtttatgct tgaaggtgcc
     2701 attatcggca ttcatgaagc tgcgctcccg gtggcacgcc gccaggagct tgcagaatgg
     2761 gctgactctc ttacgccgaa acaggagttt cctgcggaac ttgagtaatc tggcgttgca
     2821 gacgaccaat ctcaggcagt aatgcaatca ataatccaac ttgttgtacg accagaggtt
     2881 ctttgctgtc tgcccgtggt tcaagttgct gcatccgctg tttcaggcta gctaatgcct
     2941 cattgacgcg ttcttcatca gcaggttgat gatgtaacgc gtcatcaaca tagcaaactg
     3001 cgtcatcaag aaacgccaga atttcaggat tagttaactg ctcccggtga gcaccgaggg
     3061 ctgagatata gctggtaaac gtatggttaa ggcacagcaa ccgaaacgcg gcttcgcgaa
     3121 tttgcggggt aacgttcggc tcgctggaca tatttgatac caccgacgcc agctcagcat
     3181 cacggttgtg tgcatcgcgg cgggcaatac gatacgccag acggttatca cgcccctgat
     3241 ggtattgctc cagtatggca tcgagatacc gacagttggc ctctgtggcg cgttcgagca
     3301 tgcgcggcag attgcgaaac tgccagtcag gccagatgta gctcactgcc gcccacgcaa
     3361 tggcacaacc aatcagcgta tcgattacgc gaggtaacgc tacttcaaaa ccttcaccca
     3421 gtaagttaaa acacagtagc accaaaagtg tgatgaacat cgttgcatga gcgtattgca
     3481 cgttacggaa ggcaaaaaag agcacgccgg taataaccag cagcaccagc tgcccttcca
     3541 gtgatggcac aaaccacagc acaggaatgc caatggcgat acctaccagc gtaccaataa
     3601 tccttaactt caggcggtgg cgcgtggcgt tatagtttgg ctggcagaca aacaaacttg
     3661 tcagcaagat ccaatacccg tgatgcattc cggttatctg aatgatggcg tagccgaagc
     3721 acaataccag cgacattctt accgcatgac ggaagagggc ggattccggc gtgaagtgac
     3781 ggctaagacg cagccagata tcactcaacc cgtgcgggct gtcatcagcg agctcatttt
     3841 cgtcattatt atggggtagt gcctgggcct gttctgattc aattgttgcc agttgggcat
     3901 caatggcgcg taaattgttc agcaaaaatc ccagtgtttt gagtaaatcg gcgggtgcgc
     3961 cgttatcgcg catccgctcc agcgcagcat caatatgcgt aaaagcgcgc tcaaaatgcg
     4021 gatcatgttg ataaggctga cgcaacaaaa tacagcgtga cagttgctgg cacgcctggc
     4081 cctgcatcga catcagccgc tgaaaacgga acagcacgtc gctgtggcga aaatgttcac
     4141 gcaatgtttg atactgaata tgagaagagc tggcacgctc gtgaatatcc tgtgcgacaa
     4201 agtaataatg cagcgtgcga cgcgttcccc gttgaccacg atcgccacgt aagcgggtca
     4261 gcagcgagag tttcgtctga ttcaatgtcg ccatcagcag accgttggcg agagccaaat
     4321 cgtacagcgg tgcctggctt tcatcttcaa tatcaggatc aaacatgcgc gacttgagct
     4381 caagataacg cgccagttgt tcatagcaac gcgccaggtt gtcctgcagc gggcggaccg
     4441 ggaacagcag atgaccaata agtgtcagga cgttgtacca gacggcaccg gccagcagat
     4501 acatcggctg ctgataccag tgctcataca gtgatgttcc caacatagtg taaatggcga
     4561 tcagcaatgc accgaaggca attgttgcat agcgttgacc cagaccgccg agcaaaatga
     4621 agccgctggt agagagcgtt aagccaatcg caaatagcca gggccaggga aacagcaatt
     4681 ctactgaggc cgaggcgata aaaaagcaga acagcgtaat gatgaggtta cgcaaacgtc
     4741 ccgccagtcg gtcatcgaga tcggtcagcg ccgctgccac catccccagc gttagcggaa
     4801 tcgtcagttt tacatcaccc agccaccacg gaaacgctgt ggttccacaa agcgcaataa
     4861 aaatacgcgc gtaatacagc caggcgctgt tccaggtata gcgtttgagc aaaggactta
     4921 gcataaaggc catattagtg gattattcaa aacgacgacg tgcattggct tcacgcgcag
     4981 cttgcgctgt ttctaccgat accacgcgac gaccaaccgg ccatagtgca atagcggcaa
     5041 ttttaaagtt cgcaatgccg acaggaatgc caatgattga aatacattgt gcgatgcccg
     5101 ttgcaatgtg catcaggcat aaccaccagc caaagaaaat cagccagaaa atattcaata
     5161 ccgtaccgcc agtattcagc agcacatttt tgccagccgg gttcagttca tcgacatgaa
     5221 tagcttcatt gccataaggc accagagata gtttagtgat ctcccagcag gatcgtgtca
     5281 gcggtaaggt aaaaatcagc acaatactga ccagagtcgc caacagccag cccagagtgg
     5341 tggcaaatcc gccaagcaca aagttcagaa tgttcaaaac ggtacgcata aaacctcgct
     5401 ttactgtggt tttcagtaat gggcggcaat tgtaacgttt ttttgggctg gagcacgttt
     5461 tctctgacgg ttacactgat aagaaataat ttcgtgtgga tctacagagt catggaactg
     5521 aaagcgacaa cgcttggaaa acgtctggca cagcaccctt acgatcgggc ggtgatcctc
     5581 aatgccggga ttaaagtctc cggcgatcgc cacgaatacc ttattccttt caatcaatta
     5641 ctggcgattc actgtaagcg cggtctggta tggggcgagc tggaatttgt actgccggac
     5701 gaaaaagtgg tgcgtctgca cggcaccgaa tggggcgaga cgcagcgttt ttaccatcat
     5761 cttgatgctc actggcggcg gtggagtggc gagatgagcg aaattgcgtc tggtgtttta
     5821 cgccagcaac tggatttgat tgccacgcgc actggagaaa ataaatggct gacgcgtgag
     5881 caaacctctg gcgttcagca acaaatccgc caggctttgt cggcgttgcc gttgccggtt
     5941 aaccgactgg aagaattcga taactgccgt gaggcgtggc gtaaatgtca ggcctggttg
     6001 aaagatattg aaagcgctcg gttgcagcat aaccaggcgt ataccgaagc catgcttacc
     6061 gagtatgcgg attttttccg ccaggtcgag tcttcaccgc tgaatccggc gcaggcccgg
     6121 gcagtcgtta atggcgagca ttctctgtta gtgctggcag gtgcaggaag cggaaaaacg
     6181 tcggtgctgg tggcccgtgc aggctggttg ctggcgcgtg gtgaagcgtc ccctgagcaa
     6241 attttattgc tggcgtttgg tcgcaaagcc gctgaagaga tggacgagcg gattcgcgaa
     6301 cggctacata ccgaagacat taccgcacgc acgtttcatg cgctggcgct gcatattatt
     6361 cagcagggca gcaaaaaagt tccgatagtc agcaaactgg aaaatgatac cgctgcccgt
     6421 catgaactct ttattgctga gtggcgcaag caatgcagcg aaaagaaagc gcaggcgaag
     6481 ggctggcggc aatggctgac ggaagaaatg cagtggtcag tgccagaagg taacttctgg
     6541 gatgatgaaa aattacagcg tcgccttgcc tctcgcctcg atcgctgggt aagtctgatg
     6601 cggatgcacg gtggtgcaca ggcagaaatg attgccagtg cacccgaaga gattcgcgat
     6661 ctgttcagta aacgtatcaa gttgatggcc ccgttattaa aagcctggaa aggtgcgctg
     6721 aaggcagaaa acgctgtcga tttttcgggc cttattcatc aggcgattgt gattctggag
     6781 aaaggtcgct ttatcagccc gtggaagcat attctggttg atgaatttca ggatatctcg
     6841 ccgcagcggg cagcgttgtt agcggcatta cgcaagcaaa acagtcagac gacgttgttc
     6901 gctgttggtg atgactggca ggcgatttac cgattcagcg gtgcgcaaat gtcgctcacc
     6961 accgctttcc atgaaaactt tggtgaaggc gaacgttgtg atttagacac gacttaccgt
     7021 tttaacagtc gtatcggtaa ggtggcaaac cggtttattc agcagaaccc aggccagctg
     7081 aaaaagccgc taaacagctt aaccaatgga gacaaaaaag ccgtcacgtt attggatgag
     7141 agtcaacttg acgctttgct ggataagctc tctggttatg ccaaaccgga agagcgcatt
     7201 ctgatcctgg cgcgttacca tcacatgagg cctgccagcc tggaaaaagc ggcaacacgc
     7261 tggccgaagt tgcaaatcga ctttatgacc attcatgcca gcaaagggca acaggcggat
     7321 tacgtcatca tcgttggctt gcaggaggga agtgatggtt ttccggctgc ggcgcgggag
     7381 tcgattatgg aagaggcgct actgccaccg gttgaggatt tcccggacgc tgaagaacgg
     7441 cggttaatgt acgtggcgct gacccgggca cgccatcggg tatgggcact gtttaacaaa
     7501 gagaatccct ctccctttgt ggaaatactg aaaaatctgg atgtgccggt ggcgagaaaa
     7561 ccgtaagaaa caggtggcgt ttgccacctg tgcaatatta cttcagacgg tccgcgagat
     7621 aacgctgata atcggggatt agaatatcga ccgcgtcgtt gaaatgcggc gactggatta
     7681 tgaagtctgc cgttgccacg ttggtggcga ccggaatgtt ccataccgtc gccagacgca
     7741 gcaaggcttt cacgtcagga tcgtgcggca cggcatttag tggatcccag aagaaaatca
     7801 atacatcaat tttcccttct gagatcaatg cgccaacctg ctggtcaccc cccattgggc
     7861 cactcaacat cgcgttgacg ttcatgccgg tcgcgcggga aattaagtta ccggtagtgc
     7921 ctgttgcata cagtacgtgt tgttccagta acggttgatg ccgttccacc cagctcatca
     7981 gcatttgttt gcagtgatcg tgtgccacca gcgcaatatg tttccgcgca ggtaaagtgc
     8041 gagtcgtcag ttccataatg tacatccgta gttaactttc ctacagatta ctgtaagcac
     8101 ttatcgctgc aagataaaga ccgaaaaagc ctgcgcacag gcacaaaaat ctcaggaaga
     8161 tggttgtttt tccgcccact gcaggaaagt atttcgcgtt tgtgggtcag ccagtttaaa
     8221 ccaatacttc agccgttgtt ctgtgagcac ctgagactgc ggtggaatag catccagctc
     8281 ggaaacaccc gaaagcagcg tactgtcatc tgccatcatc gtggcaaatt gcggtagtga
     8341 agcgcgtttc gcggatttgt tatagcgttc aacctcgacc tcataatcaa tcgtttttgc
     8401 cgttgaggta atggcgagaa tatccagctt taccggaatc ggcgtcgcat cgccatccaa
     8461 caattcaagg cgcggcgcgg catcaaaatg gttagcttcc cgctcattct ccaggcgagg
     8521 caggcgaaaa ttgacctggt tgatgagctg ggtattaaaa ctgaccacca gcggtgggga
     8581 gatatacagc cgttcttcac tattggaaag atgaatcgtc ttctcaacgc gaaacactaa
     8641 ctgatgcggt ccattatcca gttcaatgct gtccgcaccg cgcagcagag agctggaaac
     8701 cttcttgccg tcgagtacca gaagatcgac atcggttgaa agccgcagcg tggtggcaaa
     8761 aacggatacc ggcaaacata atgcaattaa ggtggtcacg atgccggttt tcataggtag
     8821 ctcctgtcaa aagaccgccc gaataataat gtgtcaaaat ttttctttaa tc
//
LOCUS       DAJTZL010000161         8838 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.161, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000161 DAJTZL010000000
VERSION     DAJTZL010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8838
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.161"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            812..1054
                     /locus_tag="OO544_003349"
     CDS             812..1054
                     /locus_tag="OO544_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6768869.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(1384..2313)
                     /gene="fdhE"
                     /locus_tag="OO544_003350"
     CDS             complement(1384..2313)
                     /gene="fdhE"
                     /locus_tag="OO544_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HCR6768870.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(2310..2945)
                     /gene="fdoI"
                     /locus_tag="OO544_003351"
     CDS             complement(2310..2945)
                     /gene="fdoI"
                     /locus_tag="OO544_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HCR6768871.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(2942..3844)
                     /gene="fdoH"
                     /locus_tag="OO544_003352"
     CDS             complement(2942..3844)
                     /gene="fdoH"
                     /locus_tag="OO544_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HCR6768872.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(3857..6907)
                     /gene="fdnG"
                     /locus_tag="OO544_003353"
     CDS             complement(3857..6907)
                     /gene="fdnG"
                     /locus_tag="OO544_003353"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(6320..6322),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HCR6768873.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            7101..7934
                     /gene="fdhD"
                     /locus_tag="OO544_003354"
     CDS             7101..7934
                     /gene="fdhD"
                     /locus_tag="OO544_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HCR6768874.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
     gene            8087..>8838
                     /locus_tag="OO544_003355"
     CDS             8087..>8838
                     /locus_tag="OO544_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiG family protein"
                     /protein_id="HCR6768875.1"
                     /translation="MKRNLLSSAIIVAIMALGLTGCDDKKAETETPPPANSQPAAPAP
                     EAPVAKAEAKPETPAQPVVDEQAVFDEKMDVYIKCYNKLQIPVQNSLARYADWLKDFK
                     QGPTGKESTVYGIYGISESNLAECEKGVKSAVALTPALQPIDGVAVSYIDAAVALGNT
                     INEMDKYYTQENYKDDAFAKGKTLHQTFLKNLESFEPVAESYHAAIQEINDKRQLAEL
                     KNIEEREGKTFHYYSLAVMISAKQINNLISQE"
BASE COUNT         2121 a         2232 c         2265 g         2220 t
ORIGIN      
        1 atttacatca ttccctgtct atgccggtaa tattgaataa gaactctata aatttttatt
       61 cttcgttggc accatacgtg tcttgctaca tcactgttca ggagcattta aatattattt
      121 gtagagctga gtggcaatta tagcagataa tgcttaactc atcatcttag ttcaggtttg
      181 agcgattttt gatagtcaaa acctcctctg gtgccatact tttctcataa atatttttaa
      241 aatctatagc tcacaaacat tatgcaaaga taatgggctg gcatcattgt cagtatcatt
      301 catttactgc tctgtatgtt atgaatcatt atgttgtatg tttgctctta aaccgtggga
      361 aggaggctat aatggttgcc aatattaatc tcattaaaca agaaagttat tctgttgtta
      421 atttagaaaa acaactttcc cgaactgtta ccaacaaaat tataacgatg agcaagtaaa
      481 gaccggttgg tggcagtact gaccggtctg tgatgaaaga ttaatttcac cttaaatcct
      541 ggtgctgtta ggatgctgac gtttttcggc tacaccattc agattatgca aataaatatc
      601 cctgaatata atatattcat agcacccata actcatcctg ggcgataact aaaccatttt
      661 tgagtaataa atgaaacaac tgcaacgctc ttattatttc tggaaaacgc ctcgcaaaaa
      721 taaaaaatga tgcgtaaatg agctttcttt aaagattcgg caagggtact tttctctcca
      781 tagattcaaa aatgatattg gcgagatatt tatgaactct ctggcaggta tagatatggg
      841 cagaatttta ctcgatttat cgaatgaggt gattaagcaa cttgatgatc tcgaggtgca
      901 gcgtaatctt cctcgtgcgg acctattaag ggaagcagta gatcaatacc tgataaatca
      961 atcgcaaaca gcaagaacca gtgttcctgg catctggcaa gggtgtgagg aagatggtgt
     1021 cgaatatcag cgtaagctgc gcgaggaatg gtaatgggaa aaaatgatgt caatcagatt
     1081 gctgacaacg tgcgcgttgt tcatgccgga tgcggcgtga acgccttatc cggcctacaa
     1141 agtcatgcaa attcaatata ttgaagttta ttggtaggcc tgataagcgc agcgcatcag
     1201 gcaatcatac gcttgtcatc agtgacatac ccataacctt atcgatgtgt tggagggaag
     1261 aacgaggcaa acagacaatt gcggcatgat cttgaatggg taacgcggaa tacgagagat
     1321 ggtgtcgtca caccctatga gctgtaatcc gggcgacctg cgccacccgg aaaacgtcag
     1381 agattactct ccttcacccg gaaacaggaa cgggttaatg gaactacggg cgtagccttc
     1441 ttgctccatt cgcgcgtcca gtaccagaga ggcgaggtca tctgccacgg cttcaacttt
     1501 cggttctttt tcctgataaa gaatcttcag gtaagtgccg cagtcatcgc agctttcggc
     1561 tttaatcgcg gcctgttcgt catccagcga ccagtaatgc agtttgccgc tctgctcgca
     1621 gttgctgcat tttacgcgca ctacgtgcca ttcggtttca cacaggttgc agtgcaggta
     1681 acgcagaccc tgagtggtgc caatttgcac catgctggac accggcatag agccacatac
     1741 cgggcaatat tgacgttgtt cgccgtattc agcgcgggct ttgccgggga tcagattggc
     1801 catctgcgcc cagtagagcg acagcgcagc ccagataaac ggcgctttat cgctgctgac
     1861 ggacgagaaa tcagaggcaa acagcgcgct ggccatatct tccagctcct gagtcgatgc
     1921 cttctccaga ttctcaatca ctgccagcgc cgggccgctc atttcaggtt tcagctcagc
     1981 aattagcgcc atcagcagct tttgccagtg cttatcacgc ggcagaacgt gaatatccag
     2041 cgggggcttg ccctgtgcgc tggcttcttt aatgcgcgcg gtcagatcca tctccagcgg
     2101 atggtcgtac agcaccactt cctgggcgtg ggcgataagc gcagcaaagc gcaggtaatc
     2161 acccagcgga ttattttctg ccagctcgcg cagacgctcg gcgcggcggt tgtataaatt
     2221 cttgagccga gggaacaata acggcggaat catatccgcc gtacgtttct cgctcgaacc
     2281 cagctcatct tgcgggatta tgcgaatact cattcagctt tcttttccgt tgtcttgcgg
     2341 acctcacggt accagcgcgg gtgatgtttc ttcgcccagg cgctggtaac ccatccttcc
     2401 accatggcgg taatcgtgcc tttcacccaa agggcggcgt agatatgcac catgataacc
     2461 acaattaacg ccactgcggc aaatgaatgc agcattaacg cgaatcggat caccgggatt
     2521 gagaaagcag gcgcaaaata aggacgccag ataatcacgc cgctcaccag cagcagaacc
     2581 aggaaaataa tcgccgccca gaaaacgcat ttctgaccga agttataacg cccggtgtca
     2641 cctacttcct cgttgacgac gatcttacga atattcttcg cccaaaagat atcatcccga
     2701 ttgattaggt tgtgatgcca gtaacggaaa aacatgatga tgaacgaggc aaacataacc
     2761 acgccgacaa acgggtgcag aattcgcgcc agctgcggtg tgcccatgat ttgcatcaac
     2821 cagttgaagg acgggaacaa aaagcccagc ccgctcaccg ccgccaggat gaagcagaag
     2881 gcggtgatcc agtggttgat acgttccggc gcggtgtagc gcacgatggt gtcacgtcgt
     2941 ttcatttgcg ctcctcgtct ttctcttcgt gcagattatt ctcttcctca tccgcacggt
     3001 tcggaccgac acccacgtag tggaagatac tggccgcaaa ggtagccgca aagccaacag
     3061 ccgcgagcgg tttccagatg cctttccaga atttcacggt ttcgctgatt tccgggttct
     3121 ccggcaagcc atgatacaga tttggcttgt cagcatggtg cagcacgtac atgacgtgtg
     3181 taccaccgac gcctgccgga tcgtacagac ccgcattgtc gtaaccgcgg gttttcagct
     3241 cagcaacgcg ctcgctcgcc agcgttttca tcgactcttt cgtaccgaag tgaatcgcgc
     3301 ccgttgggca ggtcttcacg caggctggtt cttgcccaac caccacgcgg tcaacgcaca
     3361 gcgtacattt gtagacgcgg ttgtcttccg ggttgaggcg cggaatgtcg aacggacagc
     3421 ccgcaatgca ataaccgcag ccaatgcact gctcggactg gaagtcgacg ataccgttgg
     3481 catactgaat aattgcacct tccgccgggc acgctttcag gcagcctgga tcggaacagt
     3541 gcatacagcc gtctttgcgg atcagccatt ccagtttgtc gttctgctcc acttccgaga
     3601 agcgcatcac cgtccacgat ttggcgctta aatcattggg gttgtcgtac accccaatgt
     3661 tattgccgac ggtatcgcga atgtcgttcc actctgaaca cgccacctga caggctttac
     3721 agccgataca ggtggtgacg tcgatgagtt tcgccacttc ttcctggaag tcccgcgcct
     3781 gaggcgcggg ggtcagaccg ttagtcgcgg aacgacgaat gatatcttgc gattgataag
     3841 ccatatgtcg tctccgttac accttttcca cattcacaag gaaggactta aactccggcg
     3901 tctgcgtgtt cgcatcacca acgaatggcg ttaacgtatt ggcaataaag ccttttttag
     3961 ctacgccttc atagccccag tgaatcggaa taccgatggt atcgatatct ttgccgttcg
     4021 ctttcagcgt gcgaatacgt ttggtcacca ccgctttggc tttgatatag ccacggttgg
     4081 aggagacttt cacggtatcg ccctgggcaa tgccaagttt attcgccagc gactccccga
     4141 tttccacaaa ctgctctggt tgcaaaatcg cgttcaacag cgcgtgtttg gtccagtagt
     4201 ggaagtgctc ggtcagacga taggtggttc cgacatacgg gaacttatcg gctttaccca
     4261 atgcttcggc gtcgtcttta aagattcgcg cagccgggtt cgagataacg tttggatgca
     4321 gcgggttagt tcccagcggc gtttcaaacg gctcgtagtg ttccgggaac ggaccttccg
     4381 ccatcttatc gagggcaaac agacgcccca tgccttcctg ctgcatgata aacggcccga
     4441 cgccgctgcc cggaggcgct gcgctgtagt ccggaatatc ccagccggtc cacttagtgc
     4501 cgtcccattt cagcaactga cgcttcggat cccacgggtt accctgcgga tctgcggagg
     4561 cgcggttata cagaatgcgg cggttaagcg gccatgccca tgcccagccc agcgtgttac
     4621 cgaggccaga cggatcggcg ttatcacgac gcgccatctg gttgccttcc ggcgtccagc
     4681 taccggcgaa aatccagcag ccacaggagg ttgtaccgtc atcgcgcaat tgggcgaacg
     4741 agctaagttg ttggcctttc ttgacgataa ccgcaccggt tgccggatcg gtaatatcgg
     4801 ccagcgcctt accgttgctc tccatcgcca cttcttccga cttcggctca tgcggaatgg
     4861 cgtagttcca ggtcatgttc agcacctgat ccgggtttgc gccaccctgt tcggcataca
     4921 tcttgcgcaa gcgcaggaag ataccggaga ggatctcgcc atcagtcagc gcaatccctg
     4981 gggcgtccgc acctttccag tgccactgca accagcgacc tgagttaacg attgaaccgt
     5041 tctcttctgc gaagcaggtc gatggcagac ggaacacttc ggtctggatc ttcgacgagt
     5101 caacttcgtt cagctcaccg tggttctgcc agaagttaga ggtttcagtg ttcagcgggt
     5161 cgatggtgac gaggaacttc agtttcgaca gacaaccgat cactttgttt ttgttcggga
     5221 atgaggcaac agggttaaag ccctggcaga tatagccatt gaccttgccc tctttcatca
     5281 tctcgaagta ttgcaggacg tcgtagcctt tatcccactt cggcaaccag tcaaagcccc
     5341 agctattttc cgccgtcgct ttatcaccaa agaaggcctt catcatagag acgaagaatt
     5401 tcgggtagtt gccccagtag tttacctggc cttccagcag cggttttggc gtgttggcgg
     5461 taaggtaggt ttgcagatcg gtctgcttct cgcttggcag cgtcatgtaa cctggcaggc
     5521 tctgcgacag cagccccagg tccgtcagcc cctgaatatt ggagtgaccg cgcagggcgt
     5581 taacgccgcc gcctgccatc cccatattgc cgagcagcag ctggatcatc gccatcgtac
     5641 gaatgttttg cgcaccaacg gagtgttgcg tccagccgag ggcatacagg aacgaggcag
     5701 ttttatcgtg agcactggtt tctgcgatgt attcgcagac tttcaggaac gcgtcttttg
     5761 gcgtaccaca gatgttttca accacatctg gcgtgtagcg ggaaacgtgc tgtttcagca
     5821 agttccacac gcagcgcgga tgttgcagcg tggtatcgcg tttggcgaag ccgttttcgt
     5881 ccagttcgta agtccaggag gatttatcgt acttgcgttt ttccgcgtca tagccggtga
     5941 acaggccatc ttcaaagccg taatcctcac gcacgatcag gctggcgttg gtataggctt
     6001 cggtgtattc gcggttgaat ttttcattgt tcagcaggta cagcaatacg cctgacagga
     6061 aagcaatgtc agtaccggaa cgaatagggg catagtagtc agccaccgcc gccgtacgcg
     6121 taaagcgagg atcgatcaca atcagcttcg cgccattgtg aattttggct tccatcgccc
     6181 agcggaaccc gaccgggtga gcttcagcgg cgttaccgcc catcaccacg acgaggttgg
     6241 cgttcttgat gtcgacccag tggttggtca tcgcaccgcg accaaatgtt ggagcaagac
     6301 ttgctaccgt tggtccgtgt cagacacgcg cctggttgtc gaccgcgagc atacccagcg
     6361 cgcgggagaa tttttgcgtt aaatagccgg tttcgttact cgacgcggaa gcacacagca
     6421 tcccggtgga gagccagcgg ttaacagtca cgccttcggc gttttgcgca atgtagttag
     6481 catcgcggtc ttctttcatc agtttggcga tgcgatcaaa cgcctcttcc caactgattt
     6541 gctgccattt atcagaacct ggcgcacggt attccggaaa cttcagacgg ctttcggagt
     6601 ggatgaaatc caccaggcca gcgcctttcg gacaaagtgc accgcggttg accgggtgat
     6661 ccggatcgcc ttcgatatgg aagatagatg ctttggcgtt ttttgctccg tcaccgaggc
     6721 tgtacatcaa cagcccacag cctacggaac aataggtgca ggtattacgg gtttcgcggg
     6781 tgcgcagcag tttatactgc cgggtttccg cgagtgctac gctgggtgca aaacccagtg
     6841 ccgctgccgt ggtgcctgcc ataccgccag cgcagatctt aaagaactgc cttctgctga
     6901 cctgcatgga ttgctccttg tttcgacatt gtcacgtccc atttacattc gcttgctgcg
     6961 tgtgcaggga gtgggagtta tttttctttg cggaaggggc cgcaaaggtc cagaattggc
     7021 tcaatttccc tccatccagg aagggtttgt aacagaatac cataatgttg gtgtgtgtgt
     7081 tcttatctgg ttaagagaaa gtgaaaaaaa cacagcgaaa agaaatcgaa aatgtgacaa
     7141 atatcacagg tgttcgtcaa attgagttat ggcggcgtga tgatttacaa cacccacggt
     7201 tagatgaggt cgcggaagaa gttcccgttg cgctggtcta caacggcatt tcgcatgtgg
     7261 tgatgatggc gtcgcccaaa gaccttgagt actttgcgct cggtttttcg ctttccgagg
     7321 ggataataga aagtccgcgc gatatcttcg gcatggatgt cgttccttcc tgtaatggtc
     7381 ttgaagtaca aattgagctt tccagccgcc gctttatggg gttgaaggag cgccgccggg
     7441 cgctggcggg acgtacgggc tgtggcgtat gcggcgtgga gcaacttaat gacatcggaa
     7501 aaccggtgca gccgctaccg ttcacccaga cgtttgatct caacaaactg gatgatgcat
     7561 tacgtcatct caacgatttt cagccagtgg ggcaactgac tggttgtact cacgccgctg
     7621 cctggatgtt gccatctggc gaactggtcg gcgggcatga agacgtgggt cgccatgtgg
     7681 cgctggacaa actgttaggc cgccggtcac aagaagggga aagctggcag caaggtgcgg
     7741 tactggtttc cagtcgtgcc agttatgaaa tggtgcaaaa gtcggcgatg tgcggcgtag
     7801 agattttgtt tgcggtgtct gccgcgacca cgcttgctgt agaagtggct gagcgctgta
     7861 atctgacact ggtaggtttt tgtaaaccgg gtcgggcaac ggtttatacc catccgcagc
     7921 gtttgagcaa ttaattaaaa taagtcctgc gatatatatt aaattgcagg acttattcat
     7981 ttcgtgaatt ttattatttt atttataaat aaaacgcgtg tagtgacgtt ttcttttttc
     8041 atttattatg ttcactctct tatctatata tttataaggc aattaaatga aaaggaattt
     8101 attatcttcc gctattatag tcgccatcat ggccctcggt ctgacgggtt gtgatgataa
     8161 aaaagccgaa acagaaacgc ccccgcctgc caatagtcaa cctgccgcac cagctcctga
     8221 agctcccgtt gcaaaagcag aagctaaacc tgaaacacct gcgcaaccgg tggtcgatga
     8281 acaagcggtt ttcgacgaaa aaatggatgt ctatatcaag tgctacaaca agttacagat
     8341 ccctgtacag aacagtctgg cgcgttatgc tgactggctg aaagatttta aacaggggcc
     8401 taccggtaaa gagagtactg tttatggcat ctacggcatt agtgaatcca acctcgctga
     8461 gtgtgaaaaa ggcgtaaaaa gtgctgtggc gttaacgcct gcgctgcaac ctattgatgg
     8521 cgtggcggtg agttatattg atgctgccgt ggcgttgggt aataccatta acgaaatgga
     8581 taaatattac acccaggaaa attataaaga cgatgcgttt gcgaaaggga aaacgctgca
     8641 ccagacattc ttaaaaaatc tggaatcctt tgaacctgta gcagaatctt atcatgcggc
     8701 gattcaggaa attaatgata agcgccagct tgcggaactg aaaaatattg aagaaagaga
     8761 agggaaaaca ttccactact actctctggc agtcatgatt tcagcgaaac aaattaataa
     8821 cctgatttcg caagagaa
//
LOCUS       DAJTZL010000162         8678 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.162, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000162 DAJTZL010000000
VERSION     DAJTZL010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8678
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.162"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(91..927)
                     /gene="bglG"
                     /locus_tag="OO544_003356"
     CDS             complement(91..927)
                     /gene="bglG"
                     /locus_tag="OO544_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HCR6768876.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKCAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRIISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLPNPLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMH
                     LVSAQMSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILE
                     HASINDSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRK
                     EH"
     gene            complement(1213..1938)
                     /gene="phoU"
                     /locus_tag="OO544_003357"
     CDS             complement(1213..1938)
                     /gene="phoU"
                     /locus_tag="OO544_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HCR6768877.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
     gene            complement(1953..2726)
                     /gene="pstB"
                     /locus_tag="OO544_003358"
     CDS             complement(1953..2726)
                     /gene="pstB"
                     /locus_tag="OO544_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HCR6768878.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(2817..3707)
                     /gene="pstA"
                     /locus_tag="OO544_003359"
     CDS             complement(2817..3707)
                     /gene="pstA"
                     /locus_tag="OO544_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HCR6768879.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSVPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(3707..4666)
                     /gene="pstC"
                     /locus_tag="OO544_003360"
     CDS             complement(3707..4666)
                     /gene="pstC"
                     /locus_tag="OO544_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HCR6768880.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(4753..5793)
                     /gene="pstS"
                     /locus_tag="OO544_003361"
     CDS             complement(4753..5793)
                     /gene="pstS"
                     /locus_tag="OO544_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HCR6768881.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKEIGNKVNYQGIGSSGGVKQIIANTVDFGASDSPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDETIAKLNPGLKLPSQNIAVVRR
                     ADGSGTTFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(6041..7114)
                     /locus_tag="OO544_003362"
     CDS             complement(6041..7114)
                     /locus_tag="OO544_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6768882.1"
                     /translation="MNKYIKQWCFAVFMLSLSSVALAAPKGICTPDNGVFHRTLDFSG
                     YLITANENKVGTTFNTTVTNGSSYPGRCHCDTGNVGEFPYIYYTSKINQALTYAGVHS
                     NINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKYNCNRIEPLSISSGGKA
                     IVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMADVYIRGDITAPQSCEIN
                     NLQPVYFDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLGILNTDDISTSFYATEP
                     NTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMGKSTVYGEKSGSVTFSAAPAS
                     LTGARPAPGQFTATATITVEIVR"
     gene            complement(7125..>8678)
                     /locus_tag="OO544_003363"
     CDS             complement(7125..>8678)
                     /locus_tag="OO544_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR6768883.1"
                     /translation="VTIKEEDGSEQRFTQPYASLAILKREGQTDVDVSVGELRDEDGF
                     TPDVLQAQILHGFSHGITLYGGMQAAENYGSAALGVGKDLGALGAISFDVTHARANFS
                     HDDTETGQSYRFLYSKRFDDTDTSLRLVGYRYSTEGYYTLNEWASRRNSPEDFWETGN
                     RRSCVEGTLTQSLGRDYGNLYLTLSRQQYWHTDDVERLMQFGYSSSWKRLSWNVSWSY
                     SNTARQGTGNNHASDNTSEQIYMLSLSVPLLGWWGNSYATYSVSQNDNSGSSHQLGLS
                     GTALERNNLSWNLMQSYNSHDDEVGGNMSLTYDGSYGTVNGSYNYSQNSQRLNYGIRG
                     GILAHSEGVTLSQELGETIALVKAPGAAGLEIDNMRGAATDWRGYTVKTQLNPYDENR
                     VAISDNYFSKSNIELDNTVVTMVPTRGAVVKAEFVTHVGYRVLFRVLNANGKPVPFGA
                     IAAIQDASLADSGIVGDRGELYLSGLPEKGQVTLSWGENASTKCIFNYSLSTPESESG
                     LIEQGVTCH"
BASE COUNT         2249 a         2205 c         1954 g         2270 t
ORIGIN      
        1 ctgaaaaaaa cggcctgacg tgaatcaagc aatttttttc aggttttgcc cgcttagtgc
       61 ggtaacaatc ctttactcag taataatatt tcagtgttct ttgcgcacgc gctctatatt
      121 tatggctaaa aacataatct ctgcgggtga aattttacgt tgatactgca aaccaataaa
      181 aatggcgatc cgttccgcac attgccatgc ttgcgggtaa ttttgtttta ctgcttgttg
      241 taatgattca tcactatcgt taattgaagc atgttcaaga atacgccagg ataaaaactt
      301 cagatgtgta accagtcgct gataactcaa gctttcttcc tggtaattaa ggctgaactg
      361 aaattttatt aattgcagca tttcgcgcat taactgcgtg acacctgcaa catcctccat
      421 atttccgctc atttgggcac tgaccagatg catggcaata aagcccactt catctttcgg
      481 taactgcacg cccaaccgtt tatcaataat ggttaacgct tcttccccta gctggaactc
      541 tttcgggtaa agccgctgga tatcccacag caacgggttg ggcagcaaca cgttttgctg
      601 aaagcgttta atcgcaaact ggcaatggtc agttagcgag atataaatac tgtcctgtaa
      661 ttttcccaag cgctcctgcg ctaaagagat aatacgatca caggttgcca tcacctcaag
      721 aggaatatga cttaagagtt cgcttaatcg cccgttcagt tcatgactgc tcaaggcata
      781 ctctttttct attccacttg agttaattct ttcgccagcg catttttgaa agccaattcc
      841 tcgccccatg acgacttttt cccgctgttg atcatcaata accaccacaa cattattgtt
      901 gagaattttg gtgatttgca tgttcatagc aaggaccttt tttataaaca aaaaaaaccg
      961 acttcaccag tattctctgg ttatgtcagg ttttgcctgc gaatgcagta acaatccagt
     1021 catttattaa tgttttttat aacgaacatc caggttcgga aattaattta attgcgtgca
     1081 gagaaaatag caatgcgcta ttgataaaaa tatgaccatg ctcgcagtta ttaactttgt
     1141 gtaattttag gaatttataa agttatatat aacaaatccc aataattaag ttattgggat
     1201 ttgtctggtg aattatttgt cgctatcttt tcccgccagc agtttatcca gctcatcacc
     1261 gcctacatga cggaaatcct gccccttcac gtagtagaag ataaactcac aaatattctg
     1321 gcagcggtcg ccaatacgtt cgatagaacg cgcgcagaac agcgcagtaa gtacgctcgg
     1381 aatggtacgc gaatcttcca tcatgtaggt catcagttga cgaacaatac cttcgtattc
     1441 ctgatcgact tttttatctt cacgataaat acgtaccgct tcgtcaatgt ccatccgcgc
     1501 gaacgcgtcc agcacgtcgt gcagcatctg gatggtatga cggcccagcg actccagact
     1561 taccagcaac ggctgatgct gctgggagaa tttctccagc gcagtacggc agattttgtc
     1621 cgccacgtcg ccaatacgct ccagctcggc aatggtttta ctgatcacca taaccagtcg
     1681 caggtcgctc gccgtcggct gacgtttggc gataatgcgc acgcaggctt catcgatcgc
     1741 cacttccatc atgttgacgt tcttgtcgcc ttcgatcacg cgcttcgcca gatcgctgtc
     1801 ctggttatgc atcgcggtga ttgcatcaga aagctgctgc tccaccatgc cacccatggt
     1861 catcacctgc gtgcggatac tttccagttc ggcgttgaac tggccggaaa tatgtttatt
     1921 aagattgaga ctgtccataa cgcactcctg aattaaccgt aacgaccggt gatgtagtct
     1981 tctgtttgtt tcttcgctgg cttggtgaac agatcgtccg tgttgctgaa ctcaatcaat
     2041 tcgcccaggt acataaacgc cgtgtggtcg gaacaacgcg cagcctgctg catgttgtgg
     2101 gtaacgatca ccacggtgta atcctgcttc agttcggtga tcagctcttc aatacgcccg
     2161 gtagagatgg ggtcgagcgc cgaacacggt tcgtcgagca gcagcacttc cgggcgaatg
     2221 gcgataccac gcgcaataca cagacgctgt tgctgaccac cagagagaga gtaaccgctc
     2281 tggtgcaatt tatctttggt ttcgttccac aatgcggctt tggtcaatgc ccactgcacg
     2341 cgctcgtcca tgtcggcacg ggagagcttc tcaaacagac gaacaccaaa agcgatgttg
     2401 tcgtagatgg acatcgggaa cggcgtcggt ttctggaaca ccatgcccac tttcgcacgc
     2461 agcagtgcga tatcctgaga gttggtcagg atgttgtcgc catcaagcag aatttcacct
     2521 tctgcgcgct gctccggata cagttcaaac attttgttga aggtacgcag cagcgtcgat
     2581 ttaccgcagc cggacggccc gataaacgcc gttacctggt ttttagcgat atccaggttg
     2641 atgtttttca gggcatggaa tttgccgtag tagaagttca aattacgaac ctgaatttta
     2701 ctcggggcag tttcaaccat actcattgca atcttttcct catcgtacct gatgcttttc
     2761 gctaatcagg cttaccgtag gccgggtaag gcgttcacgc cgcatccggc aaagtgttaa
     2821 ccgtgtttat tcttcgcaaa aacaacgcgc gccagaatgt tcagcagcag gacgcacagg
     2881 gtaatgatca ataccccggc ccaggccagt tgttgccatt ccgcaaacgg gctcatcgca
     2941 aacttaaaga tcgtcaccgg caggttggcg atcggctgca tcatgtccgt gctccagaac
     3001 tggttggaga gcgcggtaaa cagcagcggc gcggtttcac cggcaatacg ggcaatcgcc
     3061 agcaggatac cggtcataat cccggacacc gacgctttca gcgtaatcgc ggagatcatc
     3121 ttccacttcg gtgtacccag cgcataagcc gcttcacgca ggctgtccgg caccagtttc
     3181 agcatgttct cggtggtgcg gataacgatc ggcacctgca acagcgccag cgcaattacg
     3241 cccgcccagc cggagaagtg ctccatctgt gccaccacga tggtgtagac aaacagacca
     3301 accacaatcg acggcacgga gagcagaatg tcgttaatga agcgaatcac ttctgccagc
     3361 caggatttac gaccatattc cgccagataa atccccgcca taatgcccag cggcgtaccg
     3421 aataccgtgg cccacaaaat taacagcccg ctacccgcca gggcgttcgc cagaccacca
     3481 ccttccgtat tgggcggcgg cgtcatttca gtgaacagcg ccagcgacat accgtcgata
     3541 ccgcgagtga tggtggacat taaaatccag atcagccaga acaggccgaa ggccatcgtc
     3601 gccatcgaga gcgtcagcgc aatacggttt ttgaggcggc gacgcgcctg cattttgcgg
     3661 cgagattcag ccagcgccgc agtggtttgc atttcaacca tagccattag cgtgccccct
     3721 cattcttagc caggcgcata atcataaact tcgatgcggc gaggacgatg aaggtaatca
     3781 caaacaggat caggcccagt tccatcagtg cggcaacgtg cagaccggat tccgcttccg
     3841 caaattcgtt cgccaacgca gaggtgatac tattgcccgg catgtacagc gaggcgctgt
     3901 cgagctggta ggtgttaccg atgataaagg tcaccgccat ggtttcaccg agcgcgcggc
     3961 ccagccccag catgatgccg ccgataacac catttttggt gaacggaaga acgatacgcc
     4021 agataacttc ccaggtggtg cagccaatac cgtaggccga ctctttcatc atcaccgggg
     4081 tttgttcgaa cacatcacgc attaccgccg caatgtacgg aataatcatg atggcgagga
     4141 tcacgcctgc cgcgaggata ccgataccaa atgcggggcc agagaacagc gcgccaacaa
     4201 tcgggatatt cgacatgata ttgccgaccg gctcctgaaa gtaaacggcg aacagcggcg
     4261 caaagataaa caggccccac atgccgtaaa cgatacttgg aatggctgcc agcagctcaa
     4321 tggcgatacc cagcgggcgt ttcagccagc caggcgcaag ctcagtcagg aacagggcga
     4381 taccgaaact caccgggacg gcgatcagca gcgcgataaa cgaagtcacc aacgtaccgt
     4441 agatcggcac cagcgccccg tagatatcgt tcggtgcatc ccactctttg gtccatagga
     4501 aagccagacc aaatttctga atgctcggcc aggaggagat gatcagagag acaataatgc
     4561 cacccaacat caatagcaca atcagcgccg ccagttttac cagcacgctg aaaattatgt
     4621 cgcccttttt acccggtggg ttaaaagcag gcttggttgc agccataagt tactcttcag
     4681 ttaaacgcgt ttacaaagtc attttgtaat gccggatgcg gcgtaaaaca ccgtacccgg
     4741 cctggagttt tattagtaca gcggcttacc gctactgtct ttaatattgg tcttccacgc
     4801 agcgcgaacc tgttcaacta cactatccgg caggctggcg taatccaggt cgttcgcctg
     4861 tttagccccg gttttgtacg cccagtcgaa gaatttcagc acttctgtgc cttgttctgg
     4921 tttcttctga tctttgtgga tcagaatgaa cgtggtagag gtaataggcc atgcatcttc
     4981 gcctttctgg ttggtcagat cctgagcgaa ggttttgctc cagtctgcac cttttgctgc
     5041 attagcgaag ttttcttcgg tcggactaac cggtttacca tcagcggaga tcagtttggt
     5101 gtacgccagg ttgttctgct tcgcgtaagc atattcaaca taaccaattg cacccggcag
     5161 acgctgaacg aacgcggcga taccgtcgtt acctttaccg cccagaccga tcggccattt
     5221 tacggtagag ccagtaccaa cgttgttttt ccactcttcg ttcactttcg ccaggtagct
     5281 ggtgaagacg aaggtagtcc cggagccatc tgcgcggcgt actacagcaa tgttttgtga
     5341 aggcagtttc agacccggat tcagtttggc gatggtttca tcatcccact tcttgatttt
     5401 gcccaggtag atgtcgccga gggttttacc atccagcacc agttcgccag acttcagccc
     5461 tggaatgtta accgccagca ccacgccgcc aatcacggtc gggaactgga acagaccttc
     5521 ctgagccagt ttttcgtcag acagcggcga gtcagaggca ccaaaatcaa cggtattagc
     5581 gataatctgt tttacgccac cggaagaacc gataccctgg tagttaactt tattaccgat
     5641 ttctttctgg taagtgtcag cccatttggc atacaccggc gcagggaagg ttgcacctgc
     5701 acctgtcagg cttgcttctg caaacacaga gaaagcgctc atcgataagg tcgcggcgac
     5761 aacagttgcg acggtggtac gcataacttt cataatgtct cctgggagga ttcataaagc
     5821 attgtttgtt ggctacgaga agcaaaatag gacaaacagg tgacagttat atgtaaggaa
     5881 tatgacattt ttatgacaga aaggaattaa caaataactt actgatttta aatgtgaaga
     5941 tgaaaatccc acatgtcatc tttttgtcat gatgcattta aaagtgataa agaaatgggt
     6001 gagatgacaa cgatgtgatg aacaatcgtt gtcagaccta ttagcgcaca atctcaacag
     6061 ttatcgtcgc tgttgcagta aattgccccg gggctgggcg agctccagta aggctcgcag
     6121 gagcagctga aaaagttaca ctacctgatt tttcaccata cactgttgat ttacccatgt
     6181 ctgttggcaa ctcaccaccg ttcactttga tttccttatt atttttgtcg taaattttta
     6241 tccccacgtt agagtttgaa gttacgacca ttgaattgtc cgtattgggt tcggtagcat
     6301 aaaaagacgt actgatatca tcggtattca gtatccccag gttttcacac tcaatagtga
     6361 ctgttttagt aattttatgt gttgttaccg cacttccaac aacagatgaa aaatctgcag
     6421 ctggtatttc tttaaaatca aaataaactg gctgtaaatt atttatttcg caactttgcg
     6481 gagctgtaat atcgccccga atataaacat cagccatcgg ctgtgagtaa tccaccgggg
     6541 tgtcacggct aattgttcca tacaatgtca ctatctttgt ttcaggaata atcaattttc
     6601 ctgcaaatgt cttcttgata taaaaatata ctatcgcttt acccccactg gatatactta
     6661 aaggttcaat gcggttgcaa ttgtactttg tattaccaga cgggttgtta gcatgatatt
     6721 caaaaggtgc attcacatac ccaaccccaa gaatatctat agcaatccca acatcaaggt
     6781 tcggattaag atcataataa ttaatattag agtgaacccc agcataagtt aatgcctggt
     6841 ttatttttga cgtataatag atatagggga attctcctac attaccagta tcacaatgac
     6901 agcgtccagg atatgaggat ccattagtta cagtggtatt aaatgtcgtt ccaaccttat
     6961 tttcgttcgc ggtaataaga tatcccgaga aatcaagcgt acggtgaaat acgccattat
     7021 caggggtaca gataccttta ggagctgcaa gtgctacact acttaacgag agcataaaca
     7081 cagcaaagca ccactgtttg atatacttgt tcataaaaat ccttttaatg acatgtcaca
     7141 ccctgttcaa ttaatccgct ctcactttct ggtgtcgaaa gtgaataatt gaagatgcat
     7201 tttgttgagg cgttttctcc ccaggataac gtaacctgtc ctttttctgg tagaccagaa
     7261 agataaagtt cgccacggtc accgacaatt cctgaatctg ccaaacttgc atcttgtatc
     7321 gcagcaatgg ctccaaaagg taccggttta ccatttgcat ttaacactct gaagagaacg
     7381 cgataaccca catgagtcac aaactccgct ttaaccactg caccacgcgt gggaaccatc
     7441 gtaacgacgg tattatcaag ttctatattc gacttcgaga aatagttatc gctgattgct
     7501 acccgatttt catcataagg gtttagctgt gtcttgaccg tatagccgcg ccagtccgtc
     7561 gcagcaccgc gcatattatc tatttctaac ccggcggccc caggtgcttt aacaagagca
     7621 atagtttcac ctaactcctg acttaacgtt accccttcgc tgtgtgccag aattccccct
     7681 ctgataccat aattcagcct ctgggaattt tggctgtagt tatagctgcc gttcaccgtg
     7741 ccataagagc catcataggt cagggacata ttaccgccaa cctcatcatc atgactgtta
     7801 taggactgca ttaaattcca tgaaaggtta tttctttcca gcgccgtacc gctgagtccg
     7861 agttgatgtg agctaccgga attatcgttt tgcgaaacag aataggtggc gtaactatta
     7921 ccccaccagc ccaataaagg aacagataaa gagagcatgt agatctgctc actggtgtta
     7981 tcactggcat ggttgttccc cgtcccctgt ctggcagtat tggaataact ccaggagacg
     8041 ttccacgaga gacgcttcca gctactgctg tagccaaatt gcattaatcg ttcgacatca
     8101 tcggtatgcc agtattgttg ccggcttaat gtcaggtata aattgccata atctctcccc
     8161 aacgactgcg ttagcgtccc ctccacgcaa ctacgtcggt tacctgtttc ccaaaagtct
     8221 tcagggctgt tgcgccgcga tgcccactca ttgagggtat agtagccctc ggtggagtaa
     8281 cgatagccaa ccaagcgcaa gctagtgtct gtgtcgtcaa atcgttttga atagagaaag
     8341 cgatatgact gacccgtttc tgtatcatca tggctaaaat tcgcacgagc atgtgtcaca
     8401 tcgaaagaaa ttgcgcccaa agcgccaaga tctttaccga cacccagagc tgcagaacca
     8461 taattttcag cagcctgcat acctccatat aaagtgatcc cgtgggaaaa accatgaagt
     8521 atttgcgcct gaaggacgtc cggtgtaaat ccgtcttcat cgcgcaattc acccacgctg
     8581 acatcaacat ctgtctgacc ttcacgttta agaatcgcta atgaagcata aggttgggtg
     8641 aatcgttgtt cgctaccgtc ttcttctttg atggttac
//
LOCUS       DAJTZL010000163         8633 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.163, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000163 DAJTZL010000000
VERSION     DAJTZL010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8633
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.163"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(143..2686)
                     /gene="mdoH"
                     /locus_tag="OO544_003364"
     CDS             complement(143..2686)
                     /gene="mdoH"
                     /locus_tag="OO544_003364"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HCR6768884.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(2679..4214)
                     /gene="mdoG"
                     /locus_tag="OO544_003365"
     CDS             complement(2679..4214)
                     /gene="mdoG"
                     /locus_tag="OO544_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HCR6768885.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            4609..5766
                     /gene="mdoC"
                     /locus_tag="OO544_003366"
     CDS             4609..5766
                     /gene="mdoC"
                     /locus_tag="OO544_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HCR6768886.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(5774..7255)
                     /gene="clsC"
                     /locus_tag="OO544_003367"
     CDS             complement(5774..7255)
                     /gene="clsC"
                     /locus_tag="OO544_003367"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HCR6768887.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRQMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLSDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(7197..7730)
                     /gene="ymdB"
                     /locus_tag="OO544_003368"
     CDS             complement(7197..7730)
                     /gene="ymdB"
                     /locus_tag="OO544_003368"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HCR6768888.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(7825..8136)
                     /locus_tag="OO544_003369"
     CDS             complement(7825..8136)
                     /locus_tag="OO544_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR6768889.1"
                     /translation="MFRPFLNSLMLGSLFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSVPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(8257..8589)
                     /gene="csgC"
                     /locus_tag="OO544_003370"
     CDS             complement(8257..8589)
                     /gene="csgC"
                     /locus_tag="OO544_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgC"
                     /protein_id="HCR6768890.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDVYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPEDRVKIVVTVSDGQALHLSQQ
                     WPPSSEKS"
BASE COUNT         2156 a         2247 c         2051 g         2179 t
ORIGIN      
        1 ataacgccgg tatttttatt ggctcgttgt ttttgccgga tgcgacgtga acgctttacc
       61 taacccacaa aagcttgcaa aatcaataaa ttgcatgaac gatgtaggcc tgataagcgt
      121 agcgcatcag gcaactacat ttttattgcg aagccgcatc cggtttacgc aatgccagtg
      181 gattgagctt tatcccttcg taataactca cccatgaaga atatctctca ggggaattcc
      241 agacgcggaa atgcagacgg gccatcgtca ccggatcgct tagcagcaca aggcgacgat
      301 cgcgattcag cttctctggc gtctcgttca gcgcctgttc aacgtggcgg tcacgggcga
      361 tttccagcac cttactggcg cggtgacgcg cggtcgccat tgcggttgcc agagcgttaa
      421 atgacgggtt aaacactgcg tgcataaagc catcgtcaag ggagcgttgg cgattcatct
      481 caaggaaccg atcggtatca accagcacct gcggcggtga atactcttcc gggatcagga
      541 acagtttcca gcgtttggtg cgcagaccaa cggtggcacg gctggaaatc accgaaacaa
      601 acggtgacag gatcaacgag aagacaatcg gtgccagcca gaacaggaaa cgcagatcca
      661 gccacgccat cccaacagcc cacactaacc ctaacagcag ctgtgagccg tggcgtttga
      721 acgcttcacc ccaggaagtg gagtcatcat cacgctgcgg tgaattccac accacttccc
      781 agccaaggaa cgcgctgacg acgaagaccg tatggaacag catgcgtacc ggagccagca
      841 gcacggaaaa cagcacttcc agcagcaacg ataatgtaac gcgccagaag ccgccgtatt
      901 ctttcgttcc tttgcaccag ataagcaaaa tgctcaataa cttcggcagg aacaacagca
      961 ccatggtcga agcaaaaagt gcaatcgcca gctcaggacg ccactgcggc cacaccggga
     1021 acaactgccg tggttgcagg aagtattgcg gttcggtcaa cgcatgtact acctgcaatg
     1081 cagtagagag cgcgaggaac ataaaccaca gcggagcgga gagataagac atcacgcccg
     1141 tcaggaacac cgcacggtga accgggtgca tacccttcac caggaacaga cggaagttca
     1201 tcaggttacc gtggcaccat cggcggtcac gttttagctc atcaagcaag ttaggcggca
     1261 attcttcata agaacccggg agatcgtaag caatccagac cccccaacct gcacggcgca
     1321 tcaacgccgc ttccacgaag tcatgtgaca ggattgaacc ggcaaaggaa ccttcgcccg
     1381 gcagcggagc cagtgcgcag tgctcgataa acggtttcac gcggataatc gcgttatgtc
     1441 cccagtagtg cgactcgcca agttgccaga agtgcaaacc ggctgtaaac agtggcccat
     1501 acacgcgggt cgcgaactgc tgacagcgcg catacagcgt atccataccg gacgctttcg
     1561 gcgacgactg aatgatcccg gcgttcgggt tggcttccat caggcgcacc agcccgcaca
     1621 aacaatcacc ggtcattacc gagtcagcat ccagcaccac catgtagctg tactggctgc
     1681 cccagcgacg gcagaagtca tcgatattac cgcttttacg cttcacgcgg cgacggcggc
     1741 ggcgatagaa aatctgacct tcgccaccga cttcagcgat aagctccatc caggcttttt
     1801 gctctgcgac gcagatatcc gggttataac tgtcactaag aatgtagaca tcaaagtgtt
     1861 tggcattccc ggtggctttt actgattccc acgttgcacg caggccagca aaaacacggt
     1921 tcacgtcttc gttacagata ggcatgatca acgccgtgcg atgctccggg tttaatggtt
     1981 catcgccaac tgttgacgca gatatactgt atttatcgcg accaataagc agttgcagga
     2041 agcccattaa cgccgtccag aatccggcgg acacccaaca gaacagtacc gcaaagagga
     2101 tcaggatacc ggtttgcagc atataaggca gaagctgcat aaaggaaacc cacaaatcct
     2161 gaccaaccat atccatagga ttaatcagcg cccaaccctg ataaggaaga atggtcttca
     2221 tataccaggt cgcgacgaca gtttgcgcga gcgtcaggat caacagaatg taacggcgga
     2281 tggtaccgac ggtacgccac ttttgctcgc tctcctgctc ttctttggtc aaacgagcca
     2341 gatagcgcgg cgtgacatcg cgtccacgca ggcgatccca gaaacggcct accgggttgg
     2401 tacgccacgg gtcgggaaac atcgaggagc gttttgcttc tggcatcgcc ttcagctgat
     2461 cgcgcccttc gtcgtcttta attaactgtc catcagcaag tgaatctggc caggcttgtt
     2521 ccagacgcgc ctttaccgag ccttgcgggg aatcatcctc ccgcgcccag gtgcggtgtt
     2581 cggcatccag cgcctgatga acggcgcgga tatcagtctt cggcaatgcc gctttctcgc
     2641 ttgcggcgat gggcattgcg tcaatgtact cagttgtctt attcattggc aggtaactgg
     2701 tagctccagg tttcactcaa cgtctgatcg gcattcacca gcgcagcacg catttcagtg
     2761 gttttcttgg catctttcac tttcacacgc atcaccagac gccagccttt ggtaaccggg
     2821 ttatagcgca ccgtgctttc aactatctca ccattatcac caatgctggt ttgcgctgtg
     2881 accggggtat cctctggcag ttttttcatc tctgcgccgg taaaatcgac cacaaaggcg
     2941 atagtaccgt caggctggcg aatcaggttc gactgcttca catcccccgt tgaacgacgc
     3001 gtttgttgca cccatgcgtt atctggcgca tgcagtttgt cttcatcacg gctgaaggtg
     3061 atggtgtatt taaagttcat ctctttaccc ggctccggca gctgatccgg cgtccagtaa
     3121 gcgacgatgt tatcgttggt ttcatcgttg gttggaattt ccaccagctc aacgctgcct
     3181 ttaccccact cccctttcgg agtcacccat gcgcttggac gaagatcgta acgatcatcg
     3241 agatcttcaa agcgggagaa atcacgaccg cgctgcaaca gaccgaagcc ttgcgggttt
     3301 tccatcgaga agctgctaac cgctaaatgt ttcgggttat tcaacggacg ccagatccat
     3361 tcgccattac cggcatggat agagagaccg ttagagtcgt gcaactccgg acgatagtta
     3421 tttgcaggcg acggttggtt cggcccaaac aggaacatac tggttaacgg tgcaaccccc
     3481 agtttgccga ctttatcgcg cagatagatt ttcgactgca catccacaac cgtgtcacgc
     3541 cctggcataa ccacgaattt gtaagcacct gtcgcgcgcg gcgagtcaag caatgcataa
     3601 atggttaaac gtttatcagt cggttttgga cgctcgatcc agaactcttt gaagcgtgga
     3661 aattcttccc ccgatggcaa ggcggtatca attgccaggc cgcgggcaga aaggccataa
     3721 acctgacctg caccaatcac gcggaaatag ctggccccga gcatgctgac gatttcatcg
     3781 tttttatctt tgctgttgat cgggtaaagc actttaaaac cggcaaaacc aaggtctttt
     3841 accgtgtctt tgtcatgctg aacatcgccg aaagtgaaat aatccgggct gtatttgatt
     3901 cgtttgactg cggtggcagt cacttcattt attttgaccg gggtatcgaa gtacataccc
     3961 tgatggtaga actcgagttt gaatggggtc ttcagattgt tccagtacgc tttgtcatga
     4021 ttaaactgga tctgctgata gtccgcgtat ttcatatcgc ggaaaacgga gggcaagttg
     4081 cttttgggcg cctcatagcc tttcccggct aaggattgag cttgctttgc gacatcatca
     4141 atactgaaag cccagcttga agatgtatac agggttaaca ttactgcagc actcaaccaa
     4201 cgcattttca tcatttgtag tttatgtttc ataattagta aagcacttcc ccctttgtgt
     4261 gcttatatcg atccgatcta ttttaatgga taaccagcca ttccgacaac agaatcccgg
     4321 tatcgttcag cacgctggct catggattaa gcacctgatt gcaaccgttt tcactttccg
     4381 tgcttctcct aaagacagaa gcatcgactt cgtgtagggt ttgccagcaa ttttatttat
     4441 caaccgtctt acagacgctt ccattctggt tagtggtagc gatgatgcta tgcataaaaa
     4501 ccttgaagcc tgcgcctggc gcggtcaggt cggtgtcgct atggcaacaa gcctaaaggc
     4561 ataacccgac agggataaga cgaaaaatcc gagattagtt aaccatatat gaatccagta
     4621 cccgcgcaac gtgaatattt cctcgactcc atccgcgcct ggctgatgtt gttagggatc
     4681 ccttttcata tttctttaat ctattcgagc catacatggc atgtgaatag cgccgaaccg
     4741 tcattgtggc tgaccctttt taatgacttc atccactcgt tccgcatgca ggtatttttc
     4801 gttatatccg gctacttttc ctacatgctt tttttacgct atcccttgaa aaaatggtgg
     4861 aaagtacgtg tcgaacgtgt aggtatcccg atgttaacag ccatcccctt actgacattg
     4921 ccgcaattta ttatgctgca atacgtcaaa gggaaagcgg aaagttggcc tgggctgtca
     4981 ttgtatgaca aatataatac gttggcctgg gaattaatat cacacctgtg gtttttactg
     5041 gtgttagtag tcatgacgac gctgtgcgta tggatattta aacgcatcag aaataattta
     5101 gaaaattctg ataaaacgaa taaaaaattc tcgatggtaa aactatcggt gattttttta
     5161 tgcctcggca tcggctatgc ggtaataaga agaacgattt ttattgtgta tccgcccatt
     5221 ctgagtaatg gcatgttcaa ttttattgtc atgcaaacgc tattttattt gccgttcttt
     5281 atcctcggtg cactggcttt cattttccct catcttaaag ccttgtttac cacgccgtct
     5341 cgtggctgta cccttgcagc agcattggca tttgtcgctt atttactcaa ccagcgctat
     5401 ggcagtggcg atgcctggat gtacgaaacc gagtcggtga tcaccatggt cctcggtctg
     5461 tggatggtga atgtggtctt ctcctttggc caccgtttgc ttaacttcca gtcagcgcgg
     5521 gtgacttact ttgttaacgc atcgctattt atctatctgg ttcaccaccc gttaacgctg
     5581 tttttcggcg catacattac accgcacatc acctccaact ggcttggttt tctctgtggc
     5641 ctgatattcg tagtagggat tgcgataatt ctgtatgaaa ttcatttacg catcccgtta
     5701 ctgaagtttt tgttttctgg taaaccggtt gttaagcgtg agaacgataa agcaccagcc
     5761 cgttaagcca catttacaat aaccattcca cgggcaatat cgacgccagt ctgaccataa
     5821 cccgcttcca gaaactggtg gcgggttctt ttttgagaat aatctctttc tttgcatgac
     5881 gatcaaccca gttgatccgt ccccacctgt ccagacggag ctgccaggcc gcatcatact
     5941 ggctctgaat aaagcgttta tcaattaact gtgccagcgt ttcgctctct atcacgaagc
     6001 ccatttcagt attgagcaat gttgaacgcg gatcgaaatt gaaagagccg ataaacaccg
     6061 ttttaccatc gatgctaaaa gttttagcat gcaggctggc tccggaatta ccggttatgc
     6121 cgcgatcgtg taacgtacta ctttgttcac gcgtcggctt gagttcatat aattccacgc
     6181 catagcggag caattttttg cgccagcgcg cgtatccggc atggacgaca gcaacatcgt
     6241 tagcggcaag agaattggtt aggatcgcaa tctttacccc ttttctcacc atccgtaaga
     6301 gttgcgccac acctgcgcgt gtcggtacaa aataggaaga gataatatcg atgcgttcac
     6361 tgggtgagcc catgatatcg gacaggcgct gcggtaacag tgaatgccgt tttgccttgc
     6421 cctccccttt cgccggatca tcacttaata aacgtgtttt cgcccagata agcggcaacg
     6481 ttccatcaac cagatgattt ataaacggac tggattccat ttggcgtaag taacgatgcg
     6541 tcatggcatc gttatgccag gaggcgggta actcgatgcg atccgccatt tcaccttccg
     6601 ggacatccag cacctgctgt aagggtgaaa ccgatttgca ataccagtag cgggcgaaat
     6661 catcggcaac gtcctctacc acgggtccta ttgccataac atctaaatcc gaaaaaagtg
     6721 gctcttcccc tgccccaaaa taggcatcac caatatttcg tcctcccacc agggtaacca
     6781 cgccatcgac agtgaaactt ttattgtgca tacggcgatt gagacgggaa aagtcggtga
     6841 tataaccaag cggacgcagc aagcgaaacg agaaaggatt aaaaagccgg acttcaatgc
     6901 ggggatggct gtcaagcaag cgtaaaatat cgtcaagtcc gggcgtattg ttgtcgtcca
     6961 gcaacaaacg gacgcgaacg ccacgctttg ctgcggctaa cagggcggaa aacagtaacc
     7021 gacccgacat atcgtcctgc cagatgtaat actgaacatc cagcgtatgc tctgccattt
     7081 cggccagacg ataccgggcg gcaaacgcat ccagactttt ctccagaggg aaaagaccac
     7141 actgaccggg atgttgcgaa cacagtggca gcaccgcgct cgccagccgg ggcaaatcat
     7201 tcatctcctt gttgggtaag gagtctttcg tagaggtggg cgttttcttc atcataacag
     7261 acaaagtata cctgttcggg taaagcgtga cgggtaataa attctgaaac ggttttaact
     7321 gcgatttcag ccgctgccgc acgagggtaa ccataaaccc cagtactgat tgcaggaaaa
     7381 gccactgacg tatagctgtt tgccgccacc agtcgcaggc tattcagata ggcatcctgc
     7441 aaaagctggt cttcgttttg ttcaccacca cgccagactg gcccgacggt gtgtactacg
     7501 gctttagcgg gaagatcgcc tgcaagcgta ataacggcat gccccgtagg gcaatcgccc
     7561 tgctgttgcc tgacttttaa acaggcatcc agcagggccg gacccgctgc gcgatgaatg
     7621 gccccatcga cgccgccgcc tcccattaat gacggattag ccgcattcac aatcacatca
     7681 acggccagtt tggtaatatc accctgcaca acatgaatac gcgttttcat aacgcctcct
     7741 tacaccttct tgtttcttaa gtgtacagca ggctttttgg ttaaatcgca tttttctgct
     7801 cagcaacgtg cgttgttcag aaacttagtt atattcgatg ttcattacag ccagcttttt
     7861 ctgtttatca agatactgca cttttactga ggctatttgc gcgtttttaa cattaccgct
     7921 cattaacgct ttgctggaat aggttttgcc cggaacagaa ccattctgtg ggcagttcat
     7981 ttctacgggt gatgactggg tgctgacgtc acatgccggt tccacaattt ggccataaaa
     8041 atggatcaca cccccttgcg cggtgcttcc tgcgatggca ataaaaggaa aaaacaaact
     8101 gccgagcata agagagttta aaaaaggtct gaacatttat tctatttcct caatgattag
     8161 tcatccttga gggttgtgtt atccatactt tcaggttggc tgattcgcag cagaccattc
     8221 tctccagatt catcttatgc tcgatatttc aacaaattaa gacttttctg aagagggcgg
     8281 ccattgttgt gataaatgaa gtgcctgtcc atcagaaaca gtaacaacta ttttcacccg
     8341 atcttccggg gaaatattta aactcaactt cgtcaaagca ataggttgat tagcaggcaa
     8401 tgagagggtc ttttcttgct tcgtctgact ttgccctgaa ctgccttcgc gcagggacaa
     8461 tatttgtact ctgcacagac aagattgagt aagagtgact tcaggaataa tggtatacac
     8521 atccccttgc tgggtcgtat taaaggttat ctggctggaa agtgccgcaa ggagtaataa
     8581 cgcattcata ttcttctccc gaaaaaaaca gggcttgcgc cctgtttctg taa
//
LOCUS       DAJTZL010000164         8618 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.164, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000164 DAJTZL010000000
VERSION     DAJTZL010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8618
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.164"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(270..1043)
                     /locus_tag="OO544_003371"
     CDS             complement(270..1043)
                     /locus_tag="OO544_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HCR6768891.1"
                     /translation="MHDQPLLRMSLPSIPSFVLSGLLLLCLPFSSFACATTSHISFSY
                     AARQRMQNRARLLKQYQTHLKKQASYIVEGNAQSRRALRQHNREQIKQHPEWFPAPLK
                     ASDRRWQALAENNHFLSSDHLHNITEVAIHRLEQQLGKPYVWGGTRPDQGFDCSGLVF
                     YAYNKILEAKLPRTANEMYHYRRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDG
                     QFIESPRTGETIRVSRLAEPFWQDHFLGARRILTEETIL"
     gene            1229..1489
                     /gene="dinJ"
                     /locus_tag="OO544_003372"
     CDS             1229..1489
                     /gene="dinJ"
                     /locus_tag="OO544_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin DinJ"
                     /protein_id="HCR6768892.1"
                     /translation="MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVARE
                     KALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDLFDKLGI"
     gene            1492..1770
                     /gene="yafQ"
                     /locus_tag="OO544_003373"
     CDS             1492..1770
                     /gene="yafQ"
                     /locus_tag="OO544_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafQ"
                     /protein_id="HCR6768893.1"
                     /translation="MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLP
                     AVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLRFERTGTHAALFG"
     gene            1926..2666
                     /locus_tag="OO544_003374"
     CDS             1926..2666
                     /locus_tag="OO544_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HCR6768894.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(2637..3404)
                     /locus_tag="OO544_003375"
     CDS             complement(2637..3404)
                     /locus_tag="OO544_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HCR6768895.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(3610..4188)
                     /gene="lpcA"
                     /locus_tag="OO544_003376"
     CDS             complement(3610..4188)
                     /gene="lpcA"
                     /locus_tag="OO544_003376"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HCR6768896.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            4428..6872
                     /gene="fadE"
                     /locus_tag="OO544_003377"
     CDS             4428..6872
                     /gene="fadE"
                     /locus_tag="OO544_003377"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HCR6768897.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLDGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALAKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            complement(6915..7361)
                     /gene="ivy"
                     /locus_tag="OO544_003378"
     CDS             complement(6915..7361)
                     /gene="ivy"
                     /locus_tag="OO544_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HCR6768898.1"
                     /translation="MFKTITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDDTYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            7542..8312
                     /gene="yafV"
                     /locus_tag="OO544_003379"
     CDS             7542..8312
                     /gene="yafV"
                     /locus_tag="OO544_003379"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HCR6768899.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPKMF
                     TSGFAMEAAASSLAQDDVVDWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAI
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMMALREYREKFPAWQDADEFRLR"
     gene            complement(8354..>8618)
                     /locus_tag="OO544_003380"
     CDS             complement(8354..>8618)
                     /locus_tag="OO544_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HCR6768900.1"
                     /translation="AEKFATAIRNHWHVENKLHWRLDVVMNEDDCKIRRGNAAELFSG
                     IRHIAINILTNDKVFKAGLRRKMRKAAMDRNYLASVLAESGLS"
BASE COUNT         2070 a         2163 c         2259 g         2126 t
ORIGIN      
        1 tttgttctcc ttaattatgt aaggagtatt tgatcatgca taagcattaa aaaacagttt
       61 caagtaatga ggaatatctc aaattttaaa cataaaatgc gaattattta gtacaaaaag
      121 tgtacttctg atgatctttc cctgggtgat ggaataacag gaaagcggct cgcaaaaacg
      181 cacagatttg taggcatgat aagacgcgtc agcgtcgcat caggcatctg cgcaccaccg
      241 ccggatgcgg cgtaaacgcc ttatccgtcc tacaaaatcg tctcttccgt caatatccgc
      301 cgcgcgccca aaaaatgatc ctgccagaaa ggttcagcta accgacttac ccgaatggtt
      361 tccccggtgc gcggtgactc aataaactga ccgtcgccca gatacacccc catatggtcg
      421 gcaatctcgc ggctgtggat atggaaaaac agcaaatctc cccggcgcag gtcgttattc
      481 gccacaatgg ttgcccgacg gtagtgatac atctcattgg ccgtacgcgg gagcttcgcc
      541 tcgaggatct tgttgtaggc ataaaaaact aacccactac aatcgaagcc ttgatcgggc
      601 cgcgtgccgc cccagacgta aggcttgcca agctgctgct ccagacggtg aatcgccact
      661 tcggtgatgt tatgcaggtg atcgctgctt aaaaagtggt tgttttccgc cagcgcctgc
      721 cagcgtctgt cactcgcctt gagcggcgca ggaaaccatt ctggatgctg ttttatctgc
      781 tcccggttgt gctggcgcag cgcccttctg ctttgggcat tgccttccac aatgtagctg
      841 gcctgttttt tcagatgggt ttggtactgt tttaataaac gcgcacggtt ttgcatccgc
      901 tggcgggcgg cgtagctgaa agagatatgt gatgtggtgg cgcaggcaaa tgaagaaaac
      961 ggcaagcaaa gcaacagtaa tcccgacaac acaaaagagg gaatcgacgg caaagacatt
     1021 ctcaataatg gctggtcatg cattgatgtt gcagtgtatc ctggcccgta cctgaacaaa
     1081 cttaataaac cattattata aaattaataa atatatttca gtttattatg taaaaacatc
     1141 cgtttattaa attactcatg gattgtcgct gttgctcatt tgagctacaa ttcaagctga
     1201 ataaatatac agcacaggag atcccccaat ggctgctaac gcgtttgttc gcgcccgaat
     1261 cgatgaagat ctgaagaatc aggcggcgga cgtactggcc gggatggggc tgaccatctc
     1321 tgacctggtt cgcataaccc tcacaaaggt cgcgcgtgaa aaggcattgc cgtttgattt
     1381 acgcgagcct aatcaattaa ccattcaatc aatcaaaaac agcgaagctg gcgttgatgt
     1441 tcataaggcc aaagacgctg atgatttatt tgataaatta ggaatttaaa tatgattcaa
     1501 agggatattg aatactcggg acaattttca aaggatgtaa aacttgcaca aaagcgtcat
     1561 aaggatatga ataaattgaa atatcttatg acgcttctta tcaataatgc tttaccgctt
     1621 ccagctgttt ataaagacca cccgctgcaa ggttcatgga aaggttatcg cgatgctcat
     1681 gtcgaaccgg actggatcct gatttacaaa cttaccgata aacttttacg atttgagaga
     1741 actggaactc acgcggcgct ctttgggtaa agcacgctgc cgatgaaatt tgtaaaccct
     1801 gcgccgacag gcttgcgtaa aactcttttg gtgataatgt gagccttcca aatccagact
     1861 attccgattt gggatctatt ttgccgtcct ggcgtgtgta accgttttat caaggaataa
     1921 gcagtatgcg taaaatcgca ttaattcttg cgatgttgtt gatcccgtgc gtttcgtttg
     1981 ccggtttgct gggcagcagt agctcgacca cgccagtgag caaagagtat aagcagcagt
     2041 tgatgggatc ccctgtctac atccagatct tcaaggaaga acgtacgctc gatctctacg
     2101 tcaaaatggg cgagcaatat cagctgctcg acagttataa aatctgtaaa tattccggcg
     2161 gcttagggcc aaaacagcgt cagggcgatt tcaaaagccc ggaagggttt tatagcgtcc
     2221 agcgtaatca gttaaaacca gacagccgtt actacaaagc gattaatatt ggtttcccca
     2281 atgcctatga ccgtgcgcat ggttatgaag ggaaatacct gatgatccac ggcgattgtg
     2341 tttccatcgg ctgctacgca atgaccaatc agggtattga tgagatcttc cagttcgtta
     2401 ctggtgcgct ggtgtttggt cagccgagcg tgcaagtgag tatttacccg ttccgcatga
     2461 ccgacgccaa tatgaagcgc cataaatatt ccaactttaa ggacttctgg gagcaactga
     2521 agccgggtta cgactacttt gagcaaaccc gtaagccacc gaccgtttct gtggtcaatg
     2581 gtcgttacgt ggtcagcaag ccgttaagcc acgaagttgt gcagccacaa ctggcatcaa
     2641 actacacgct ccccgaggca aaataagcgc cattcgcctg gcataatctt ttgccaggtt
     2701 tcattgcccg tcagcggctg tgttgcaatc accgtgacca catcattcgg tgtggtctgc
     2761 gagctgaagt cgatttccac atcctgatcc agcagcgttg ccacgccaaa cggcgcgcgg
     2821 cgggtgatcc agtgtaaatt agtcgagcaa tacgccatca catagcgccc gtcagaaagc
     2881 agcatgttga aaacgccctt ctgccgcagt tcatccgcca gtgaggcgat atatttaaac
     2941 actgccgcca tgttgcccgg tgtgcgcggg taacgctgcg ttaatttatg caggagccag
     3001 cagaaggctt tttcgctatc ggtttcaccg accgggcgga agttgccggt ttccagtgat
     3061 ttgtagcccg tcagttgtcc gttatgggca taagtccagt tacgccccca taactcacgg
     3121 gtaaacgggt gagtattttc cagcgccacc tcgccccgat tggcctggcg aatatgagcc
     3181 accaccgaac aggattttat cgggtagtcc tggacaagtt tggcgatggg ggaattaaag
     3241 ctgggttgtg gatctttaaa tgtgcgacag cctttacctt cgtaaaaggt aatgccccag
     3301 ccatctttat gtggcccggt tccaccacca cgctgcacca gaccggtgaa actaaagcag
     3361 atatcggtag ggacgttggc gctcatcccg agcaattcgc acatgggtaa cctccacaac
     3421 agcgggagcg catggccccc ggcgaagatt gatttaagaa ttttcaggcc ggatgcggcg
     3481 taaacgtctt attcggccta cggttcgcgt acagatttgt aggcatgata agacgcatca
     3541 gcgtcgcatc aggcatcagc gcacaagtgc cggatgcggc gtaaacgcct tatccggcct
     3601 acgccagact tacttaacca tctctttttc aattaactgg atcaggatat ggatcacttt
     3661 aatgtgaatc tcctgaatgc ggtcggcata accaaagtgc ggaacgcgaa tttcgatatc
     3721 cgccgtgcca gccattttac cgccgtcttt accggtcagg gtgatcactt tcatcccctt
     3781 ctcacgcgcc gctgcgatcg ctttgatcac gtttgcagag ttaccggagg tggagatccc
     3841 cagcagtaca tcgccttcgc gacctaccgc ttcaacgtag cgggagaaaa tatcattgaa
     3901 accgaaatca ttaccgacgc aggaaatatg actaacgtca gaaatagcaa tcgccgggta
     3961 gcccggacgg ttttcacggt agcgaccggt caactcttcg gcaaagtgca tagcgtcgca
     4021 atgggaaccg ccgttgccgc aggaaagcac tttgccaccg gctttaaagc tgtctgctaa
     4081 caggaccgcc gcgcgctgaa tggcgtgaat attggcgtca tcttttaaaa agttagccag
     4141 cgtttccgcc gcttcgttca gttcgttacg aataagatcc tggtacatga ggatatcctt
     4201 cagcataaat gtaatagaca aaatgcagtg taccggatac cgccaaaagc gagaagtacg
     4261 ggcaggtgct atgaccagga ctttttgacc tgaagtgcgg ataaaaacag caacaatgtg
     4321 agctttgttg taattatatt gtaaacacat tgctaaatgt ttttacatcc actacaacca
     4381 tatcatcaca agtggtcaga cctcctacaa gtaaggggct tttcgttatg atgattttga
     4441 gtattctcgc tacggttgtc ctgctcggcg cgttgttcta tcaccgcgtg agcttattta
     4501 tcagcagtct gattttgctc gcctggacag ccgccctcgg cgttgctggt ctgtggtcag
     4561 cgtgggtact ggtgcctctg gccattatcc tcgtgccatt taactttgcg cctatgcgta
     4621 agtcgatgat ttccgcgccg gtatttcgcg gtttccgtaa ggtgatgccg ccgatgtcgc
     4681 gcactgagaa agaagcgatt gatgcgggca ccacctggtg ggagggcgac ttgttccagg
     4741 gcaagccgga ctggaaaaag ctgcataact atccgcagcc gcgcctgacc gccgaagagc
     4801 aagcgtttct cgacggcccg gtagaagaag cctgccggat ggcgaatgat ttccagatta
     4861 ctcatgagct ggcggatctg ccgccggagt tgtgggcgta ccttaaagag catcgcttct
     4921 tcgcgatgat tatcaaaaaa gagtacggcg gactggagtt ctcagcttat gcccagtctc
     4981 gcgtgctgca aaaactctcc ggcgtgagcg ggatcctggc gattaccgtc ggcgtgccga
     5041 actcattagg cccgggcgag ctgctgcaac attacggcac tgacgagcag aaagatcact
     5101 atctgccgcg tctggcgcgt ggtcaggaga tcccttgctt tgcactgaca agcccggaag
     5161 cgggttccga tgcgggcgcg attccggaca ccgggattgt ctgcatgggc gaatggcagg
     5221 gccagcaggt gctggggatg cgtctgacct ggaacaaacg ctatattacg ctggcaccga
     5281 ttgcgactgt gcttgggctg gcgtttaaac tctccgaccc agaaaagctg ctgggtggcg
     5341 cagaagattt aggtattacc tgtgcgctga tcccgaccac cacgccgggc gtggaaattg
     5401 gtcgtcgcca cttcccgctg aacgtaccgt tccagaacgg accgacgcgc ggtaaagatg
     5461 tcttcgtacc gatcgattac atcatcggtg ggccgaaaat ggccgggcaa ggctggcgga
     5521 tgctggtgga gtgcctctcg gtaggccgcg gcatcaccct gccttccaac tcaaccggcg
     5581 gcgtgaaatc ggtagcgctg gcaacgggcg cgtatgctca cattcgccgt cagttcaaaa
     5641 tctctatcgg taagatggaa gggattgaag agccgctggc gcgtattgcc ggtaatgcct
     5701 acgtgatgga tgctgcggca tcgctgatta cctacggcat tatgctcggc gaaaaacctg
     5761 ccgtgctgtc ggctatcgtt aagtatcact gtacccaccg cgggcagcag tcgattattg
     5821 atgcgatgga tatcactggt ggcaaaggca ttatgctcgg gcaaagcaac ttcctggcgc
     5881 gtgcttacca gggcgcaccg attgccatca ccgttgaagg ggctaacatt ctgacccgca
     5941 gcatgatgat cttcggacaa ggagcgattc gttgccatcc gtacgtgctg gaagagatgg
     6001 aagcggcgaa gaacaatgac gtcaacgcgt tcgataaact gttgttcaaa catatcggtc
     6061 acgtcggtag caacaaagtt cgcagcttct ggctgggcct gacgcgcggt ttaaccagca
     6121 gcacgccaac cggcgatgcc actaaacgct actatcagca cctgaaccgc ctgagcgcca
     6181 acctcgccct gctttctgat gtctcgatgg cagtgctgga cggcagcctg aaacgtcgcg
     6241 agcgcatctc ggcccgtctg ggggatattt taagccagct ctacctcgcc tctgccgtgc
     6301 tgaagcgtta tgacgacgaa ggccgtaatg aagccgacct gccgctggtg cactggggcg
     6361 tacaagatgc gctgtatcag gctgaacagg cgatggatga tttactgcaa aacttcccga
     6421 accgcgtggt tgccgggctg ctgaatgtgg tgatcttccc gaccggacgt cattatctgg
     6481 caccttctga caagctggat cataaagtgg cgaagatttt acaagtgccg aacgccaccc
     6541 gttcccgcat tggtcgcggt cagtacctga cgccgagcga gcataatccg gttggcttgc
     6601 tggaagaggc gctggtggat gtgattgccg ccgacccaat tcatcagcgg atctgtaaag
     6661 agctgggtaa aaacctgccg tttacccgtc tggatgaact ggcgcacaac gcgctggcga
     6721 aggggctgat tgataaagat gaagccgcta ttctggtgaa agctgaagaa agccgtctgc
     6781 gcagtattaa cgttgatgac tttgatccgg aagagctggc gacgaagccg gtaaagttgc
     6841 cggagaaagt gcggaaagtt gaagccgcgt aattgaataa cggagccgaa aggctccgtt
     6901 tctttatccg ctaattattt aaaattaaag ccatccggat ggttttccag gctgccggtc
     6961 aacgccgcga acagcaccgt tttaccatca atcgaaagcg catcgttcac attcagccag
     7021 gtgagtttct cttgcgacgt tttctcatca atagtcgaga acagccccgt catctgatta
     7081 gatttctcgg accacatcac agcgatacgt tgcgagccac agtcatgcgg tttgcacgcg
     7141 ctcatcacct gatacgtatc atctcccaac gttacggttt gtgcgggagt ataagtaccg
     7201 cctttcatca cccaggcagg cagcttatgc ccttgtacca tctgattaaa tgcagctttg
     7261 gtggtttcgc cctttgcaag gctgctaatg gttaaatcat cctgcgccat tgcactggtg
     7321 gcgatgacca gagcggcgac tgtcgttatt gtcttaaaca tcattcctcc cgagcttatc
     7381 ctgcccatgt tattaacctt ctaaagcctg gcgataacac gataatttgc aaatcagata
     7441 tttccaatat cagcatttgt tatcaattta ttctgcgcta aggcaactgc ctgataaggt
     7501 gttaaagtta taaaaccctt cgcataaagg agttgtacac cgtgcctggt ttgaagatta
     7561 cgcttttgca gcaaccactg gtgtggatgg atggccctgc caacctgcgt cattttgatc
     7621 gtcaactgga aggtattacc gggcgcgatg tgatcgttct accgaagatg tttaccagcg
     7681 gctttgccat ggaagcggca gcttcgtcgc tagcacaaga tgacgtagtg gactggatga
     7741 cagctaaggc gcagcagtgc aatgcgctga ttgcaggcag tgttgcatta caaacggagt
     7801 ctggttcggt taaccgcttt ttgctggttg agccgggcgg cacggtacat ttttatgata
     7861 agcgtcatct gttccgcatg gcagatgagc atctacatta taaagcgggc aatgcgcgag
     7921 tgattgtgga atggcgcggc tggcgtattt tgccgttggt gtgctacgac ttacgttttc
     7981 cggtgtggtc gcgcaatctc aacgattatg acctcgccat ctacgttgcc aactggcctg
     8041 ctccgcgctc tctgcactgg caggcattgc tgacggcccg cgcgattgag aatcaggcgt
     8101 atgtggcggg atgcaatcgc gtcggcagcg atggcaacgg ctgccattat cgcggtgaca
     8161 gccgggtgat taatccacaa ggcgagatta tcgctactgc cgacgcgcat caggcaacgc
     8221 gcattgatgc ggagctgtcg atgatggcat tgcgggaata tagagaaaag tttccggcat
     8281 ggcaggatgc ggatgagttt aggttgcggt gagtgggggt tgtgtttaag gctgggggag
     8341 agtcggggta agactacgaa agcccgctct ccgcaaggac tgacgccaga tagtttctgt
     8401 ccatagctgc ttttcgcatc ttacgtctta accctgcctt gaatacctta tcattcgtca
     8461 aaatattaat agcgatgtgc cgtatccctg aaaataattc tgctgcattt cctcttctta
     8521 ttttgcagtc gtcttcattc attaccacgt ccagacgcca gtgcagctta ttctccacgt
     8581 gccagtggtt tcggattgct gtggcgaact tctctgcg
//
LOCUS       DAJTZL010000165         8326 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.165, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000165 DAJTZL010000000
VERSION     DAJTZL010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8326
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.165"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            267..1925
                     /locus_tag="OO544_003381"
     CDS             267..1925
                     /locus_tag="OO544_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HCR6768901.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNMEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIVTTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(1922..2878)
                     /locus_tag="OO544_003382"
     CDS             complement(1922..2878)
                     /locus_tag="OO544_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HCR6768902.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            3029..4144
                     /gene="macA"
                     /locus_tag="OO544_003383"
     CDS             3029..4144
                     /gene="macA"
                     /locus_tag="OO544_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HCR6768903.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            4141..6087
                     /gene="macB"
                     /locus_tag="OO544_003384"
     CDS             4141..6087
                     /gene="macB"
                     /locus_tag="OO544_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HCR6768904.1"
                     /translation="MTPLLELKDIRRSYPAGNEQVEVLKGISLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(6160..6384)
                     /gene="cspD"
                     /locus_tag="OO544_003385"
     CDS             complement(6160..6384)
                     /gene="cspD"
                     /locus_tag="OO544_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HCR6768905.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            6789..8027
                     /locus_tag="OO544_003386"
     CDS             6789..8027
                     /locus_tag="OO544_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR6768906.1"
                     /translation="MSSAIHRLSDTLLRKLSGSPTTKNAFFNDGGNLSVRHSTSGLLT
                     WYFTYRAGTGRQVSPERLRLGNYPDLSLKAAREKAAQCRAWLAEGKNPRYELNRAVQD
                     ALAPVTVKEALTYWLESYAKEKRTDYESLKSRINKHIISQIGALPLEKCELRHWLACF
                     DQMAKRSPVSAGFLLQVCKQALKYCRKRRYAISNVLDDMVVGDVGKKAEISERVLTNK
                     ELGELLRALDEKIYPPYYSALIRLLIVFGCRATELRRSEVQEWDFKEMLWTVPKEHSK
                     TKVAIFRPIPEAILPFVTKLVEQNRHTGLLLGELKGQSSVSEYGRTAHRRINQAPWTL
                     HDIRHTFTTMLNDLGVDPHVVEQLTAHQLPGVQRVYNHSRYLDAKRDALNLWVERLEL
                     LQNNDEKIVVMTPRIYSQNS"
BASE COUNT         2057 a         1960 c         2233 g         2076 t
ORIGIN      
        1 ggttgcccca tatagtcatt tgtctgattg acagtgtagt gcacgcaaaa gatttaatcc
       61 tttaggcgta ataaaaaata atatatcatg ctaattattt gattttgttg tttttgcaga
      121 cttatcagca agagggagta taacgcgatt attcgctcat ttttcagaca tttgccatgc
      181 ttaaatgtga tgtcatcacg tattagcaag gcctttcccg ttatactgcc agcgtaaagg
      241 ataagtcaca tatttctgga ggggatatga ttcttgagcg cgttgaaatt gtgggttttc
      301 gcggtatcaa ccgtttgtcg ttgatgctgg aacaaaacaa cgtcctgatt ggggagaacg
      361 cgtggggtaa atccagcttg ctggacgcct taaccctgct gctatcgcca gaatcagatc
      421 tctaccattt tgagcgcgac gatttctggt tcccgccggg agatatcaac gggcgagaac
      481 atcatctgca tattattttg accttccgcg aatcgctgcc aggccgacat cgggttcgcc
      541 gttatcggcc gctggaagcg tgctggacgc catgcaccga tggctatcac cgtatttttt
      601 atcgtctgga aggggagagt gcggaagacg gcagcgtgat gacactgcgc agttttctcg
      661 ataaagacgg acatccgatt gatgtcgagg atattaacga tcaggcacgc catctggtgc
      721 gtttaatgcc ggtgctgcgc ttgcgtgatg cccgttttat gcgccgtatt cgtaacggca
      781 cggtgccaaa tgtccctaat atggaagtca ccgcgcgcca gctcgatttc ctcgcccgtg
      841 agttatcctc acatccgcaa aatctctctg atgggcagat tcgtcaggga ctttccgcaa
      901 tggtacagct gcttgagcat tatttctctg agcagggggc cggacaggcg cgatatcgtt
      961 taatgcggcg gcgagccagc aatgagcaac gaagctggcg ctatctggat atcatcaacc
     1021 ggatgattga ccgacctggt gggcgctcgt atcgggttat tttgctcggc ctgtttgcta
     1081 ctttgttgca ggcaaaaggc acattgcgac tggataaaga cgcccgtcca ttgttgctga
     1141 tcgaagatcc agaaacccgt ttacacccca ttatgctttc agttgcctgg catctgttga
     1201 atcttctgcc attgcagcgc attgtcacca ccaactcggg tgagttgctt tcgttaacgc
     1261 cggtagagca tgtttgccga ctggtacgtg agtcctcgcg cgttgccgcc tggcgtctgg
     1321 ggccgagtgg cttgagtacc gaagatagcc gacgcatatc ctttcacatt cgttttaacc
     1381 gtccgtcatc gctgtttgca cgctgctggt tgctggtgga aggggaaacg gaaacctggg
     1441 ttatcaatga actggcgcgt cagtgcggac atcattttga tgccgaaggg atcaaggtca
     1501 ttgagtttgc ccagtccggg ctaaagccac tggttaaatt tgcccgccga atggggattg
     1561 aatggcatgt actggtcgat ggcgatgaag cagggaagaa atatgccgct acggtacgca
     1621 gcctgttgaa taacgatcgg gaagccgaac gagaacattt aacggcgtta ccggcgctgg
     1681 atatggaaca ttttatgtat cgccagggat tttccgatgt gttccaccgc gtggcgcaaa
     1741 tcccggaaaa tgtaccgatg aatctacgca aaattatctc gaaagcgatc catcgctctt
     1801 ccaaacccga tcttgccatt gaagtggcaa tggaggcagg acgtcgtggt gtggactccg
     1861 taccgacgct gctgaaaaaa atgttctcac gcgtgctgtg gctggcgcgc ggtcgcgcgg
     1921 attaaccgcg aaacatcgtg gccatttgtg gctgaatagc gtcgagcatc tcatagcgcc
     1981 gacggtattc agcccgtttt ttactggcga tttcggcaat ctcttttcgt gctatctgtg
     2041 ctggaaggcg gtaatggcgt tcagcatcac atacgccgcc aaccgattcc cagaaagcgt
     2101 tgtaatcagc gtggatcttg ccttctttat cgcgataacg caggctgcgg taaatatgcg
     2161 tttcattgct gacggcaata atctgctcta cctgcaaacg ttgggcaaac agacaggccg
     2221 cttccatcac gaggcgtttg ggaaatagcc cgtggcaggc tttcgtcgca ttctggattt
     2281 cctgatgtgg aatttcccat tttgcgcctt gcagtccgcc aataaacatc gttcttttcc
     2341 cctgatattc acacagggta aacgtgatct ctgccagagg aataccttcg ctgttgcgaa
     2401 acaggattgt gctgtcacct tctttatcca ttgagatcat catggtcagc tcaagcgtga
     2461 actgctcgcc gtttttgcct tccagcttcg ccagttgcag cccgggggta ttcaaatata
     2521 agctgaattc ttccgccgac atacatccgc ggagtaacgc ataatggtaa cgtaacgcct
     2581 ccagcaattg cttacggcta agattcgccg caaggtaagg gcgatgcaga cgcacaggca
     2641 gtcgcggttg gcgcgttaac aacacattga gattaggcca gtgggaaagt tcgttcatcc
     2701 actcaacgct taaacgcggc ataatcaacg agcgcagcaa aaatttctgg cgaaaactac
     2761 ggcgatgcca gaatttaccc ggccgacact gtccacgtgc cagactaaga aaaagtgaca
     2821 ggctgctgag agattcagat ggcgtaaagg tccgttcagt tagctgcgac atattcatga
     2881 aatcaatggt tatacatgac gtcgatttca ccattgcgta tcttaaccaa acatcaatag
     2941 tgtgattact aacgtaaatt ttatggtttt gttgatattt cgttgaagtt aatgacccgg
     3001 attggcatat ggagtattca gaatatttat gaaaaagcgg aaaaccgtga agaagcgtta
     3061 cgttattgcg ctggtgatag tcatcgccgg actgattacg ttatggagaa ttcttaacgc
     3121 acccgtgccg acttatcaga cactgattgt gcgccccggt gatttacagc aaagcgtgct
     3181 ggcgaccgga aagctggacg cgctgcgtaa ggttgacgtg ggcgcgcagg tcagcggtca
     3241 gttgaaaacg ctgtcggtgg cgattggcga taaagtaaaa aaagaccagc ttttaggggt
     3301 cattgatcct gaacaggctg aaaaccagat caaggaggtc gaagcaacgc tgatggagct
     3361 acgcgcgcag cggcagcagg cggaagcgga gctgaaactg gcgcgggtga cgtattcccg
     3421 tcagcaacgt ctggcacaaa cgcaggcggt ttcacagcag gatctcgaca ccgccgcgac
     3481 ggagatggct gtgaaacagg cgcaaattgg caccattgac gcgcaaatca agcgcaatca
     3541 ggcttctctc gatacggcta aaaccaatct cgattacact cgcatcgttg ccccgatggc
     3601 cggggaagtc acgcaaatca ccactctgca aggccagacg gtgattgccg cacaacaagc
     3661 accgaacatt ctgacgctgg cagatatgag caccatgctg gtaaaagcgc aggtttctga
     3721 agcggatgta atccacctga agccggggca aaaagcctgg tttacggtac ttggcgatcc
     3781 actgacgcgc tacgaggggc aaatcaagga tgtactaccg acgccggaaa aggttaacga
     3841 cgctattttc tattacgccc gttttgaagt ccccaacccc aatggtttgc tgcggctgga
     3901 tatgactgcg caagtgcata ttcagctcac cgatgtgaaa aatgtgctga cgatccctct
     3961 gtcggcgtta ggcgatccgg ttggcgataa tcgttataaa gtcaaattgt tgcgtaatgg
     4021 cgaaacacgc gagcgtgaag tgacgattgg cgcacgtaac gataccgatg ttgagattgt
     4081 caaagggctg gaagcgggcg atgaagtggt gattggtgag gccaaaccag gagctgcaca
     4141 atgacgcctt tgctcgaatt aaaggatatt cgtcgcagct atcctgccgg taatgagcag
     4201 gttgaggtgc tgaagggcat cagcctcgat atttatgcgg gtgagatggt cgcgattgtt
     4261 ggcgcttcgg gttccggtaa atcgaccctg atgaatattc tcggctgcct ggataaggcc
     4321 accagcggca cctatcgcgt agccggtcag gatgttgcca cgctggacgc cgatgcgctg
     4381 gcgcaactgc gccgcgagca tttcggcttt attttccagc gttaccattt gctttcgcat
     4441 ttaaccgccg agcagaacgt tgaagtaccc gccgtctatg ctggtcttga gcggaaacag
     4501 cgactgcttc gtgcccagga gttgctgcaa cggctggggc tggaagaccg tacagagtat
     4561 tatccggcac agctttcggg tggtcagcaa cagcgcgtca gcatcgcgcg ggcattgatg
     4621 aacggcggtc aggtaattct tgccgatgaa ccaaccggcg cactggacag ccattctggc
     4681 gaagaggtga tggcgatcct gcatcagctg cgcgatcgcg ggcatacggt gattatcgtc
     4741 acccacgatc cgcaggtcgc tgctcaggcc gagcgggtga tcgaaattcg cgacggcgaa
     4801 attgtgcgca atcctcccgc cattgaaaaa gtgaatgttg ctggcgggac ggagcctgtt
     4861 gtcaatacag tgtctggctg gcggcagttt gtcagcggtt ttaacgaggc gctgacgatg
     4921 gcatggcggg cgctggcagc gaataaaatg cgtactttac tgaccatgct ggggattatt
     4981 atcggtattg cgtcggtggt ttccattgtc gtggtgggtg acgccgccaa acaaatggtg
     5041 ctggcggata ttcgttctat tggtacgaat actattgatg tctatcccgg gaaagatttt
     5101 ggcgatgacg atccgcaata tcagcaggcg ctgaagtacg acgacttaat cgccatccaa
     5161 aaacaaccgt gggtcgcctc agccacacct gccgtctcgc aaaacctgcg cctgcgttat
     5221 aacaatgttg atgttgctgc cagtgccaat ggcgtgagcg gcgattattt taatgtctat
     5281 ggcatgacct tcagtgaagg aaacaccttt aatcaggagc agctgaacgg tcgtgcgcag
     5341 gtcgtggttc tcgacagtaa tactcgccgc cagcttttcc cccataaagc agatgtggtt
     5401 ggcgaggtga ttctggtcgg caatatgccc gccagagtca ttggtgtggc ggaagaaaaa
     5461 cagtcgatgt ttggtagcag taaagtgctg cgtgtctggc taccttacag cacgatgtcc
     5521 gggcgagtta tgggccagtc gtggcttaac tccattactg tcagggtgaa agaaggattt
     5581 gacagcgccg aggcggaaca gcaactcacg cgtttacttt cactgcgcca cggtaagaag
     5641 gatttcttta cctggaacat ggacggcgtc ttgaaaactg ttgaaaagac cacacgtact
     5701 ttacaactgt ttctgacgct ggtggcggtg atttcgctgg tggtgggtgg tattggtgta
     5761 atgaatatta tgctggtgtc agtgaccgag cggacgcggg aaattggcat tcgcatggct
     5821 gtaggtgcgc gagcaagcga tgttttgcaa cagttcctga tcgaagccgt actggtttgc
     5881 ctggtcggtg gcgcgttggg aataactctg tcactgttaa ttgctttcac cttgcagctt
     5941 ttcttacccg gctgggagat tggtttttca ccgttggcac tgctgctggc gtttctctgc
     6001 tcgacggcca ccgggatttt atttggatgg ttacccgcac gaaatgcggc acgactggat
     6061 ccagtagatg ctctggcacg agagtaattt ttgagataaa aatgccagcc gatcgggctg
     6121 gcattttgcc tttaggatgt acacaatgag acagaagagc tatgcgactg ccgcttctac
     6181 ttcgacgggc acaataacac tggcgtgatt gccttttggc ccctggtgga catcaaactg
     6241 aacggattgt ccagctttta gcgttctata accatccatc tgaatggtgg aataatgagc
     6301 gaaaatatct tcgccgccgc cttcagggca gatgaaacca aaccctttgg cattgttgaa
     6361 ccacttaaca gtaccctttt ccatgcttcg acatccttcg caaatcttat acaagtaaga
     6421 tggaataaac cggggtcaga gagggggctg ttcaaaacct cgccaactct agaaatacaa
     6481 tttagagaat tagggcgagc cgtcaagcat ttgacagggg ccagggggca ggtatgaatc
     6541 aaaaatttga agcagttaac gctattgaca ggaatgtgac agatgtcgct gatgccaacg
     6601 atagatgata gttatttatc atctagtgtt cgcgggtgtt cgtggtagtg ttttgtgtgc
     6661 attcatcgct atgattataa aggaaaattt tgttttcctt gttcgctctt gttcgtcacg
     6721 gtttgccctt gtacgatgca cggtgtagtc aatggtgtag ttaattttga tgaccggaaa
     6781 cgcgaaacat gagcagcgca attcatagac ttagcgacac tcttttacga aaattaagcg
     6841 gatcaccaac cacaaaaaac gcctttttta atgacggtgg caatcttagc gtaagacatt
     6901 ccaccagcgg cctgttaacc tggtatttca cttacagggc cggaactggc aggcaggtat
     6961 caccggaacg cctgagactg ggcaactatc ccgatctgag cctgaaagca gccagggaaa
     7021 aagcggcaca gtgtcgcgcc tggctggctg aggggaaaaa tccgcgctat gagcttaatc
     7081 gtgctgtaca ggatgcgtta gcccccgtga cagttaagga agcgctcact tactggcttg
     7141 aatcgtatgc aaaggaaaag cgcactgact acgaatcact gaaaagccgg atcaataaac
     7201 acataatcag ccagattggc gctttgccgt tggaaaaatg cgagctacgg cactggttgg
     7261 catgttttga ccagatggca aagcgaagcc cagtatctgc cggatttttg ctacaggtat
     7321 gcaaacaggc gcttaagtac tgccgaaaac gacgatacgc aatcagcaac gttcttgatg
     7381 atatggttgt cggtgatgtg ggaaaaaaag cagaaataag cgagcgcgta ctaacaaaca
     7441 aggagcttgg ggaattactc cgcgccctgg atgaaaaaat ttacccaccg tactacagcg
     7501 ccctgattcg cctcctgatt gtcttcggat gccgtgccac tgagctaaga cgctctgagg
     7561 tccaggaatg ggattttaaa gaaatgctct ggaccgtgcc gaaagaacac agcaaaacga
     7621 aggtagcaat attcaggcca ataccggaag cgatcttgcc gttcgtaacg aagctggtgg
     7681 agcagaacag gcacacggga ttattgctcg gagaactgaa agggcaatca tccgtatcag
     7741 agtacggaag aacggcacac agacgcatta atcaagcccc ctggacgttg cacgacatcc
     7801 ggcacacgtt tacaacgatg ctgaacgatt taggcgtgga tcctcatgta gtggagcagc
     7861 taaccgcgca ccagttgcca ggagtgcaac gagtctataa tcactcccgt tatctcgatg
     7921 caaaacgtga cgctcttaat ctatgggttg agcgtctcga gcttctccag aacaatgatg
     7981 aaaaaatcgt tgttatgacc ccgcgaattt actcccaaaa ttcttgacaa attacggctg
     8041 tttttcttgc gaaaaatggc cctctaaaat aaatcgtcga atatcggcaa atatcgacga
     8101 tttatatgtg tcaactatcg aaaaaaattg aaatgtcttc ttctgagttc ttattctctg
     8161 tttcttgatt attccaatac ctgatggttt atctatttgt ttttaatgag gtaattcagg
     8221 tgtcatgcca aatggcgaca ttttttgttg cgtcgcttaa aaactcgtga cacgatttta
     8281 aacactaacg agcttctacg aaccttagcg aacaaagcct ttagca
//
LOCUS       DAJTZL010000166         8214 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.166, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000166 DAJTZL010000000
VERSION     DAJTZL010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8214
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.166"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(215..1105)
                     /gene="dgcZ"
                     /locus_tag="OO544_003387"
     CDS             complement(215..1105)
                     /gene="dgcZ"
                     /locus_tag="OO544_003387"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HCR6768907.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYGLCQFGRWIDHLGPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FDAFQEGLLSFTAALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNTDPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKAAN
                     DEEACRAGVRICQLVDNHAITHSEGHINITVTAGVSRAFPEEPLDVVIGRADRAMYEG
                     KQTGRNRCMFIDEQNVINRV"
     gene            complement(1360..1752)
                     /gene="ydeI"
                     /locus_tag="OO544_003388"
     CDS             complement(1360..1752)
                     /gene="ydeI"
                     /locus_tag="OO544_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HCR6768908.1"
                     /translation="MKFQSIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKNMHDGASVSLRGNLISHKGEDRYVFRDKSGEINVVIPAAVFDGREV
                     QPDQMINISGSLDKKSAPAVVRVTHLQK"
     gene            2028..2546
                     /locus_tag="OO544_003389"
     CDS             2028..2546
                     /locus_tag="OO544_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HCR6768909.1"
                     /translation="MNINKDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQNYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
     gene            complement(2591..4636)
                     /gene="dcp"
                     /locus_tag="OO544_003390"
     CDS             complement(2591..4636)
                     /gene="dcp"
                     /locus_tag="OO544_003390"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HCR6768910.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDGNDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLHRQWRGKAPQIMPMLQHRGLNI"
     gene            4773..5519
                     /gene="ydfG"
                     /locus_tag="OO544_003391"
     CDS             4773..5519
                     /gene="ydfG"
                     /locus_tag="OO544_003391"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HCR6768911.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWCNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            5608..6294
                     /gene="ydfH"
                     /locus_tag="OO544_003392"
     CDS             5608..6294
                     /gene="ydfH"
                     /locus_tag="OO544_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YdfH"
                     /protein_id="HCR6768912.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            6471..6674
                     /gene="ydfZ"
                     /locus_tag="OO544_003393"
     CDS             6471..6674
                     /gene="ydfZ"
                     /locus_tag="OO544_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HCR6768913.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(6710..8170)
                     /locus_tag="OO544_003394"
     CDS             complement(6710..8170)
                     /locus_tag="OO544_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCR6768914.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIIHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAKAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVGDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRYAAYGLMLQEQAP
                     TLKVQGVDLQDYANRLIERYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
BASE COUNT         2171 a         2055 c         1870 g         2118 t
ORIGIN      
        1 cggagataat ccctcaccta accggcccct tgttacagtt gtgtacaagg ggcctgattt
       61 ttatgacggc gaaaaaaaac cgccagtaaa ccggcggtga atgcttgcat ggatagattt
      121 gtgttttgct tttacgctaa caggcatttt cctgcactga taacggatcg ttgacacagt
      181 agcatcagtt ttctcaatga atgttaaacg gagcttaaac tcggttaatc acattttgtt
      241 cgtcaataaa catgcagcga tttcttccgg tttgcttacc ctcatacatt gcccggtctg
      301 ctcttccaat gaccacatcc agaggctctt caggaaatgc gcgactcaca cctgctgtca
      361 cggtaatgtt gatatgccct tcagaatgtg tgatggcatg gttatcgact aactggcaaa
      421 ttctgacacc tgcacgacat gcttcttcat cattagccgc tttaacaata atgataaatt
      481 cttcgccccc gtagcgataa accgtttcgt aatcacgcgt ccaactggct aagtaagttg
      541 ccagggtgcg taatactaca tcgccgatta aatgcccgta ggtatcatta accaatttaa
      601 atcggtcaat atccaacaac attaaataaa gattcagagg atcagtgttg cgtaactgat
      661 gatcaaagga ttcatcaaga acccgacgcc ccggcaatcc cgtcaaaaca tccatattgc
      721 tacggatcgt cagcaaataa attttgtaat cggttaatgc cgcagtaaaa gaaagcaacc
      781 cctcctgaaa ggcgtcgaaa tgcgcgtcct gccagtgatt ttcaacaata gccagcatta
      841 attcccgacc acagttatgc atatgttgat gggcagaatc cattagccga acgtaaggta
      901 attcatcgtt atcgagtggc cccagatgat caatccaccg accaaactgg cacagtccat
      961 aagaatggtt atccgttatt tctggcttac tggcatctct cgcgaccacg ctgtgaaaca
     1021 tactcaccag ccactggtag tgggcatcga tagccttatt gagatttaac aagatggcat
     1081 caatttccgt tgtcttcttg atcattgcca ctcctttttc acagttcctt gtgcgcgcta
     1141 ttctaacgag agaaaagcaa aattacgtca atattttcat agaaatccga agttatgagt
     1201 catctctgag ataacattgt gatttaaaac aaaatcagcg gataaaaaag tgtttaattc
     1261 tgtaaatcac ctctgcatta tcgtaaataa aaggatgaca aatagcataa cccaataccc
     1321 taatggccca gtagttcagg ccatcaggct aatttatttt tatttctgca aatgagtgac
     1381 ccgaacgacg gccggcgcgc ttttcttatc cagactgccg ctaatgttga tcatctggtc
     1441 cggctgaact tctcgtccat caaagacggc cgcaggaata acaacattaa tttcaccgct
     1501 cttatcgcga aaaacgtaac ggtcctctcc tttgtgagaa atcaaattac cgcgtagtga
     1561 aaccgaagcg ccatcgtgca tgttttttgc gaaatcaacg gtcatttttt ttgcatcatc
     1621 ggttccgcga tagccatctt ctattgcatg aggcggcggt ggcgctgcat cctgttttaa
     1681 accgccctgg tcatctgcca acgcataagg catgacaaga aaacttgcta atacaatgga
     1741 ctgaaatttc atactaactc cttaattgcg tttggtttga cttattaagt ctggttgcta
     1801 tttttataat tgccaaataa gaatactgcc aattgttata aggcatttaa aatcagccca
     1861 ctagctgtca aatattcaga gaatttgact cactaaagtt aagaagattg aagagtctta
     1921 aacatgtttt cagaataatc ggatttatat gtttgaaaat tattatattg gacgagcata
     1981 cagaaaaagc aaatcacctt tacatataat agcgtggaca aaaaacagtg aacattaata
     2041 aagataaaat tgtacaactc gcagataccg acactattga aaacctgaca tccgcgttga
     2101 gtcaaagact tatcgcggat caattacgct taactaccgc cgaatcatgc accggcggta
     2161 agttggctag cgccctgtgt gcagctgaag atacacccaa attttacggt gcaggctttg
     2221 ttactttcac cgatcaggca aagatgaaaa tcctcagcgt aagccagcag tctcttgaac
     2281 gatattctgc ggtgagtgag aaagtggcag cagaaatggc aacaggtgcc atagagcgtg
     2341 cggatgctga tgtcagtatt gccattaccg gctacggcgg accggagggc ggtgaagatg
     2401 gtacgccagc gggtaccgtc tggttcgcgt ggcatattaa aggccagaac tacactgcgg
     2461 ttatgcattt tgctggcgac tgcgaaacgg tattagcttt agcggtgagg tttgccctcg
     2521 cccagctgct gcaattactg ctataaccag gctggcctgg cgatatcccc aggccagcca
     2581 ttggtggtgt ttatatgttc aagccacgat gttgcagcat cggcataatc tgaggagcct
     2641 taccgcgcca ttgtcgatgc aggcgttcca gatcttcgct gttacctctg gaaaggatcg
     2701 cctcgcgaaa acgctgccca ttttcacgcg ttaatccgcc ctgctcaaca aaccactggt
     2761 aaccatcatc ggccaacatt tgcgtccaca gataagcgta ataacctgca gcatatccgc
     2821 caccaaaaat atgggcgaaa taactgctgc gatagcgtgg cggtatagca ggaagatcca
     2881 tattttccgc caccagcgcc cgcaattcaa aatcatcgac atcctgcatt gcttcgtttt
     2941 cttccaggca atgccagcgc atatcgagaa gtgcggcgct aagcagttcg ctcatctcat
     3001 accctttgtt gaacaggctg gcattacgca ttttctgttg cagttcgtca ggcattgctg
     3061 ccccgctctg ataatgccgg gcgtagcgag cgaatacctg cggatgcgtt gcccagtgtt
     3121 cgttgatttg cgacggaaat tcgacaaaat cacgcggcgt gttggtgccg gaaagcgtgg
     3181 cataacgctg gcgggcaaaa aggccgtgca gcgtatgacc aaattcatgg aataaggtta
     3241 ttacatcatc ccagagtaac aacgcaggct caccggcagc gggtttctga taattgcaga
     3301 cgttataaat taccggatgc gtttcattaa gcgttgattg ctcaacaaaa ttgcccatcc
     3361 atgcaccgcc gctttttgaa tcacgggcga agaaatcacc gtaaaataac gccagcccca
     3421 cgccattatg atcaaaaatt tcccacaccc gaacgtcagg atggtagaca ggaatatcaa
     3481 aacgttcgac aaacttaata ccgaagagct gattcgcggt ccagaataca ccttcattca
     3541 acaccgtgtt taattcaaaa tatggcttga gctgcgcttc atcaagatca aatttctccc
     3601 gccgtacctg ttccgcataa aatgcccagt cccacggctg cgcgctaaac ccgccctgct
     3661 gcttatcgat aaccgcctgt atggaggcta attcatcgct cgcacgttga cgcgccgctg
     3721 gaacaatttc ccgcataaag ttgagtgctg cttctggcgt ttttgccatc tgatcggcga
     3781 ttttccatgc ggcataatga ggaaaaccaa gcagtttcgc ctgctgtgcg cgaatctcta
     3841 ccagacgttg aatgatagcg cgggtatcat tgccatcatt tttttccgct cgcgtccagc
     3901 ccgcaataaa cagtttttca cgcgtcgcac gatcgcgcat ttcggcaagc gccggttgtt
     3961 gggtggtatt cagcagcgga atcagccatt tgttatccag acctttctcg cgagccgcct
     4021 ctgccgccag cgcaatctct tgctcactca ttcctgccag ctgcgcgata tcgttcacaa
     4081 ccagaccgcc ggatttattt gctgccagta atcgctggtt aaactggctg gtcagggtcg
     4141 cagcttctgt attcagtact tttaattttg ctttatcagc ttgcgcaagt ttggctccgg
     4201 caaggacaaa acgttgatga atcacctcca ccaggcggat ggattcacta tcaagcccca
     4261 gggattcacg gcgctgccag acagcatcta cccgcgcgaa taattcaccg ttcagataga
     4321 tatcattagc cagttccgcc agttcagcgg aaaactgttc atcaagacgc tgtaattcat
     4381 cattggtatg cgccgcagtc atcgcaaaaa agacgctggt aacgcgggta agtaattctc
     4441 cgctttgttc cagtgccaga atagtattgt tgaaatcagg cgtttgcggg ttaagcgcga
     4501 tggcagcaat ttctgcccgc ttttgctgca ttccctcatc gaatgccggg cgatagtgat
     4561 gattggcaat ttgatcaaaa tggggagcca gatacggtag tgtgctttgc acaaggaaag
     4621 gattcattgt tgtcattttc ttctcctgaa cgcgagatgt tccatagcgt aggcttactg
     4681 ataacggagt gcaatcttgc aaaccagtat tacccgctct taagcatcac gtgctatgtt
     4741 attgacacac aaaagcgttg aggaacagtg agatgatcgt tttagtaact ggagcaacgg
     4801 caggttttgg tgaatgcatt actcgtcgtt ttattcaaca agggcataaa gttatcgcca
     4861 ctggccgtcg ccaggagcgg ttgcaggagt taaaagacga actgggagat aatctgtata
     4921 tcgcccaact ggacgttcgc aaccgcgccg ctattgaaga gatgctggca tcgcttcctg
     4981 ccgagtggtg caatattgat atcctggtaa ataatgccgg cctggcgttg ggcatggagc
     5041 ctgcgcataa agccagcgtt gaagactggg aaacgatgat tgataccaac aacaaaggcc
     5101 tggtatatat gacgcgcgcc gtcttaccgg gtatggttga acgtaatcat ggtcatatta
     5161 ttaacattgg ctcaacggca ggtagctggc cgtatgccgg tggtaacgtt tacggtgcga
     5221 cgaaagcgtt tgttcgtcag tttagcctga atctgcgtac ggacctgcat ggtacggcgg
     5281 tgcgcgtcac cgacatcgaa ccgggtctgg tgggtggcac cgagttttcc aatgtccgct
     5341 ttaaaggcga tgacggtaaa gcggaaaaaa cctatcaaaa taccgttgca ttgacgccag
     5401 aagatgtcag cgaagccgtc tggtgggtgt caacgctgcc tgctcacgtc aatatcaata
     5461 ccctggaaat gatgccggtt acccaaagct atgccggact gaatgtccac cgtcagtaat
     5521 ttttataccc ggcgtaactg ccgggttatt gcttgtcaca aaaaagtggt agactcatgc
     5581 agttaactca ctcacaagca agaacgaatg accgtcgaaa cgcaacttaa tcccacacag
     5641 cctgtcaatc agcaaattta tcgtattctt cgtcgcgaca ttgtccattg cctgattgca
     5701 ccaggcacac cgttgtcgga aaaagaagtt tctgttcgtt tcaatgtgtc acgccagccg
     5761 gttcgtgaag cctttattaa actggcggaa aacggcctga ttcaaattcg tccgcagcgt
     5821 ggcagctacg tcaacaaaat ttccatggcc caggtgcgca atggcagttt tatccgtcag
     5881 gccattgagt gcgcggtggc gcgtcgggcg gcgagcatga ttaccgaaag ccagtgctac
     5941 caactggaac aaaatcttca ccagcaacgc attgccattg agcgcaagca actggatgat
     6001 ttttttgaac ttgatgacaa cttccatcaa ctcctgacgc agattgccga ctgtcaactg
     6061 gcgtgggata ccattgagaa cctgaaagcg accgttgatc gcgtgcgcta tatgagtttc
     6121 gaccacgttt ctccaccaga aatgctgtta cgccagcatc ttgatatttt ctctgccctg
     6181 caaaaacgtg atggcgatgc ggtagaacgt gcaatgacgc aacatttgca ggaaatcagc
     6241 gaatccgtgc gccagatccg ccaggaaaat agcgactggt ttagcgaaga gtaattcatt
     6301 tcctctcatc ccatccgggg tgagagtctt ttcccccgcc ttatggctca tgcatgtatc
     6361 aaaaaagatg tgagcttgat caaaaacaaa aaatatttca ctcgacagga gtatttatat
     6421 tgcgcccgtt acgtgggctt cgactgtaaa tcagaaagga gaaaatacct atgacgacct
     6481 acgatcgtaa ccgtaacgca atcaccactg gcagccgtgt tatggttagc ggcaccggtc
     6541 acactggcaa gatcctgtcg attgatactg aaggtctgac cgctgagcaa atccgccgcg
     6601 gaaaaaccgt agttgttgaa ggttgtgaag agaaactggc acctctggac ctgattcgtc
     6661 tcggcatgaa ctaagcgtgt gaatgccgcc gatggcggca ttgctttttt tacttcacgg
     6721 aatatttcgc cacggtcgct ttcgcgccat gcgctaataa agacaagtac gcttccgtca
     6781 cttttgcagt aaacaaacta ttgtctggca aatcatcacc aaagatcgcc ttaatcgcca
     6841 gcaatgactg gacgcgcgct ttcccttcag cactactttg tacagccttc tgaataacag
     6901 gtaacagtgg gtcactgatt tcaatcggat ttccctgttc atcaacacca ccgacataac
     6961 gcatccaacc cgcgacgccc agtgccagca gatcgaactt gctgtcatgc gccagatgcc
     7021 agcgaacaga atccaacatc cgctgtggca atttctggct gccatccatc gcaatctgcc
     7081 aggttcgatg acgtaacgcc gggttgctat agcgttcaat taatcggtta gcgtaatctt
     7141 gcaaatcaac gccctgcact ttcagcgtcg gcgcttgttc ctgcaacatc aagccatacg
     7201 ccgcataacg ataatgttca tcttccatgc agtcattaat gtgctgatat ccagcaagat
     7261 aacccaaata cgccaggaat gaatgactgc cgttgagcat gcgcaacttc atctcttcat
     7321 aaggcagcac atcactaacc agttcggctc ccgctttttc ccattctgga cgtccggcaa
     7381 caaagttatc ttctattacc cactggcgga aaggttcaca ggcaacgccc gcaggatcgc
     7441 ccacaccggt aagttgttcg attttcgcca gcgtatcctc tgtcactgcg ggcacaatac
     7501 ggtccaccat tgttgatggg aaagtcacgt tatcttcgat ccattgtgcc agttttacat
     7561 caacagcttt cgcgtatgaa gtgacaacgt cacgcataac atgaccgttt tctggcatgt
     7621 tgtcacatga catgacggta aatgcgggaa gtcctgccgc tttacggcgg gccagcgcct
     7681 caacaatcac ccctgttgct gttttcggct ggtggggatt ttgcacgtcg gcagcgacca
     7741 tcgggtgatc gagcattaac tgtccggtcg ccggagagtg gaaataccct ttttcggtga
     7801 ttgtcagaga gacaatcgcg atttgcggtt cacacatcgc agccaacacg gtttctaagc
     7861 catccatctg tacgtgcaag gctttcttaa cgacgccaac gacgcgagcc gtccacgcat
     7921 cggccgacat ttccgcaacg gtataaagat tatcttgctg ttgtaaatcg gcaatttgct
     7981 gttcgccgcc gattaagttg acctcataat atccccagtc actgaaatgt tctgtagcaa
     8041 gaatatcggc atacacgccc tgatgcgcac ggtgaaatgc accaaagcct aaatgaataa
     8101 ttcttggggc caggttatta cgatcataaa cagggagtgt cgcttttgct gataacaaat
     8161 tatttcccat aacaattcct taaatataaa tatggcaaac tatatgtttt gtta
//
LOCUS       DAJTZL010000167         8182 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.167, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000167 DAJTZL010000000
VERSION     DAJTZL010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8182
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.167"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..2847
                     /gene="yfaL"
                     /locus_tag="OO544_003395"
     CDS             <1..2847
                     /gene="yfaL"
                     /locus_tag="OO544_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HCR6768915.1"
                     /translation="ADGKTLVIGNTENDGAVDSIAGTGLITKTGSGDLVLNADNNDFT
                     GEMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDKYQNQAELNVGSTQQT
                     FVHSLTGFQNGTLNIDAGGNVTVNQGSFAGTIEGAGQLTIAQNGSYVLSGAQSMALTG
                     DIVVDDGAVLSLEGDAADLAALQDDPQSIVLNGGVLDLSDFSTWQSGTSYNDGLEVSG
                     SSGTVIGSQDVVDLAGGDNLHIGGDGKDGVYVVVDASDGQVSLANNNSYLGTTQIASG
                     TLMVSDNSQLGDTHYNRQVIFTDKQQESVMEITANVDTRSTTTEHGRDIEMRADGEVA
                     VDAGVDTQWGALMADSSGQHQDEGSTFTKTGAGTLELTASGTTQSAVRVEEGTLKGDV
                     ADIFPYASSLWVGDGAAFVTGADQDIQSIDATSSGTIDISDGTVLRLTGQDTSVALNA
                     SLFNGDGTLVNATDGVTLTGELNTNLETDSLTYLADVTVNGDLTNTSGAVSLQNGVAG
                     DTLTVNGDYTGGGTLLLDSELNGDDSVSDQLVMNGNTAGNTTVVVNSITGIGEPTSTG
                     IKVVDFAADPTQFQNNAQFSLAGSGYVNMGAYDYTLVEDNNDWYLRSQEVTPPSPPNP
                     NPTPDPDPTPDPDPTPDPAPTPAYQPVLNAKVGGYLNNLRAANQAFMMERRDHAGGDG
                     QTLNLRVIGGRYHYTAAGQLAQQEDTSTVQLSGGLFSGRWGTDGEWMLGAVGSYSDNQ
                     GDSRSNMTGTRADNQNHGYAVGLTSSWYQHGNQKQGAWLDNWLQYAWFSNDVSEHEDG
                     TDHYHSSGIIASLEAGYQWLPGRGVVIEPQAQVIYQGVQQDDFTAANHARVSQSQGDD
                     IQTRLGLHSEWRTAVGVTPTLDLNYYHDPHATEIEEDGSTISDDAVKRRGEIKVGVTG
                     NISQRVSLRGSVAWQKGSDDFAQTAGFLSMTVKW"
     gene            complement(2975..3697)
                     /locus_tag="OO544_003396"
     CDS             complement(2975..3697)
                     /locus_tag="OO544_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-demethylubiquinone
                     3-O-methyltransferase/2-octaprenyl-6-hydroxy phenol
                     methylase"
                     /protein_id="HCR6768916.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            3844..6471
                     /gene="gyrA"
                     /locus_tag="OO544_003397"
     CDS             3844..6471
                     /gene="gyrA"
                     /locus_tag="OO544_003397"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HCR6768917.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVEGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            6679..>8182
                     /locus_tag="OO544_003398"
     CDS             6679..>8182
                     /locus_tag="OO544_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HCR6768918.1"
                     /translation="MVLLSAGVWALQYAGSGPEKTLSPLVVHNNLQIDLNEPDLFLDS
                     DSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIEGSIRRIVYEHDLTLKDKLFS
                     SLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPLLFAATSDSQLSKTEISSIKI
                     NSETIPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFKDDQQDTEATAIAGDLLSGKK
                     RWQASFGLEERAAEKTPVRQRIVVSARLLGFGYQRLMPSFAGVRFEMGNDGWHSFVAL
                     NDESASVDTSFDFTPVWNSMPAGASFCVVVPYSHGIAEEMLSHISQENDKLNGALDGA
                     AGLCWYEDSKLQTPLFVGQFDGTAEQAQLPGKLFTQNIGAHESKAPEGVLPISQTQQG
                     EAQIWRREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLAMQNKTLLFSLDDTLVNNALQ
                     TLNKNRPAMVDVIPTDGIVPLYINSQGVAKLLRNETLTSLPKNLEPVFYNA"
BASE COUNT         1979 a         2030 c         2321 g         1852 t
ORIGIN      
        1 gccgacggaa aaacgctggt tattggcaat acagagaatg acggagctgt tgactctatt
       61 gctggtaccg ggttgatcac caaaacaggt tccggcgatc tggtacttaa tgcagataac
      121 aatgacttta ctggtgagat gcagattgaa aacggtgaag ttaccctggg ccgcagcaac
      181 tccctgatga atgtcggcga tacgcattgc caggacgatc cgcaagactg ctacggtctg
      241 acgataggga gtattgataa gtaccagaat caggcagagc taaacgttgg ctccacccaa
      301 caaacctttg tgcactcatt gacgggcttt cagaatggca ctttaaatat cgatgctggt
      361 ggcaatgtta ctgttaatca aggcagtttt gctggcacca tcgaaggtgc tggtcagctc
      421 accattgcgc aaaacggcag ctacgtgctg tcgggggcgc agtcgatggc gctaaccggc
      481 gatatagtcg ttgatgatgg tgcggtgctt tcgctggaag gcgacgcggc agatctcgcc
      541 gctctccagg acgatccgca gtcgatcgtg ttaaacggcg gtgtgctcga tctctctgat
      601 ttctccacct ggcagagcgg cacatcatac aatgatggcc ttgaagtcag tggcagcagc
      661 ggaacggtta tcggcagtca ggatgtggta gatcttgcag gtggcgacaa tttgcatatc
      721 ggcggcgacg ggaaagatgg cgtctacgtg gtggtcgatg cgagcgacgg gcaggtaagt
      781 ctggcaaaca ataatagtta tttgggcaca acacaaatcg cctccggtac gctgatggtg
      841 agcgacaact cgcagcttgg agatacccac tataaccgcc aggttatctt taccgataag
      901 caacaagaaa gcgtgatgga gattactgcc aatgtcgata ctcgctctac gacgactgag
      961 catgggcgtg atattgaaat gcgcgccgac ggtgaagtgg cagttgatgc gggggtagac
     1021 acgcagtggg gcgcactgat ggctgacagc agcgggcagc atcaggatga gggtagcaca
     1081 tttactaaaa cgggggcggg tacgctggag ctgaccgcca gcggtacaac gcagtcggca
     1141 gtacgtgtcg aagaaggcac cctgaaaggc gatgttgcgg atatctttcc ttatgcttcg
     1201 tcactgtggg tcggtgatgg ggcggcgttc gttactggcg cggatcagga tattcagtct
     1261 atcgatgcta cttccagcgg cactatcgat atcagcgatg gtacggtttt gcgtctgacc
     1321 gggcaggata cttccgtcgc ccttaatgcc tcattgttta acggcgatgg gacgctggtg
     1381 aatgctaccg atggcgtgac gttgacaggt gagcttaata ccaaccttga aactgacagc
     1441 ctgacctatc ttgccgacgt gacggttaat ggtgatctga ccaatacgtc cggtgcggtt
     1501 agcctgcaaa atggcgtcgc tggcgatacg ctgacggtaa acggtgatta taccggcggc
     1561 ggtacgctac tgctcgatag cgaattaaac ggcgatgact cggtaagcga tcaattggtg
     1621 atgaacggta atactgctgg caataccacg gtggtggtta actctattac agggattggt
     1681 gagccgacat cgacaggcat taaagtggtt gatttcgcag ctgatcccac gcagtttcaa
     1741 aacaatgcgc agttcagtct ggcaggcagc ggctacgtca atatgggagc gtatgactac
     1801 acgctggtgg aagataacaa cgactggtat ctgcgatcgc aagaagtaac gccgccatcg
     1861 ccacctaatc ccaacccgac tcccgatcct gatcccacac cggatcctga cccaaccccc
     1921 gacccggccc ctacgcctgc ttaccagccg gtgctgaatg ccaaagttgg cggttatctc
     1981 aataacctgc gggcggcaaa tcaggcgttt atgatggagc gacgcgatca cgctggtggc
     2041 gatggtcaga cgctgaattt acgtgttatc ggcggacgtt atcattacac ggcggcaggg
     2101 caactggctc agcaggaaga cacttctacg gtgcagctta gcggcggctt gtttagcggg
     2161 cgctggggca cggatggcga gtggatgctt ggggcggttg gtagctacag tgataatcag
     2221 ggcgacagcc gctcgaatat gaccggaact cgcgccgata accagaacca cggttatgcc
     2281 gttgggctga cctcaagctg gtatcagcac ggtaatcaga agcagggggc ctggctggat
     2341 aactggttgc agtacgcgtg gtttagcaat gatgtttctg aacatgaaga tggcacagat
     2401 cattaccatt cgtcggggat tatcgcctcg ctggaagccg ggtatcagtg gttaccgggg
     2461 cgtggtgtgg tgattgaacc gcaggcgcag gtgatttatc agggcgtgca gcaggatgat
     2521 tttaccgccg ctaaccatgc gcgcgtgtca caatcgcagg gggatgatat tcagacgcgg
     2581 ctgggtttac acagcgaatg gcgtaccgcg gttggtgtca caccaacatt agatctgaat
     2641 tattatcacg atccccatgc gacggaaatt gaagaagatg gcagcactat cagtgacgat
     2701 gcggtgaagc gacggggtga aataaaagtg ggagtcacgg gcaatatcag tcagcgagtg
     2761 tccctgcgcg gcagcgtggc gtggcagaaa gggagtgatg attttgccca gacggcaggg
     2821 tttttgtcga tgacggtgaa atggtaatcg ctgtctgacc atcacaattg ccggatgcgg
     2881 cgtaaacgcc ttatctggcc tacggagagc atttgtaggc ctgataagac gctgcaagcg
     2941 tcgcatcagg cattggcgcg gccaaacatc aacctcactt attctgcgtg tgcagcatat
     3001 agttcacatc cacgccgggg ccgagcttaa aggaattagt gatcgggttg taatgtagcc
     3061 cggttatatg tcgctctttt aaactggtct gatccaccca gccaagcagc tctgccggtt
     3121 taataaactt cttcacatca tgcgtacctt tgggcaccat gcgcaaaata tattccgcgc
     3181 caaccaccgc catcagccat gacttgccgt tgcggttaag cgtcgagaaa aagacatcgc
     3241 cgcctggttt caccagttgc gcacaggctc tgaccactga ctgcggatcg ggtacgtgct
     3301 ccagcatctc catgcaggtc accacatcat actgcccggc atgttttgcc gcgtgctctt
     3361 ccacggtttc ctgcacgtaa tccacctgaa tgccgctttc cagcgcgtgc agctttgcca
     3421 cctgcaatgg ctcaaagccc atatccagac cggtcaccgt cgcgccttcg cgcgccatac
     3481 tctcggccag aatgccgccg ccacaaccga catcgagcac ctttttgcca aataaaccgc
     3541 cagcacgctc ggcaatatag cccagacgca gcgggttaat gcggtgcaac ggtttgaact
     3601 caccttcaag atcccaccag cgggaggcga cggcttcaaa tttagcgatc tcttcgtggt
     3661 ctacgttatg gttttccggc gatttttcgg cattcattgg cacttctact ccgtaattgg
     3721 caagacaaac gagtatatca ggcattggat gtgaataaag cgtataggtt tacctcaaac
     3781 tgcgcggctg tgttataatt tgcgaccttt gaatccggga tacagtagag ggatagcggt
     3841 tagatgagcg accttgcgag agaaattaca ccggtcaaca ttgaggaaga gctgaagagc
     3901 tcctatctgg attatgcgat gtcggtcatt gttggccgtg cgctgccgga tgtccgagat
     3961 ggcctgaagc cggtacaccg tcgcgtactt tacgccatga acgtactagg caatgactgg
     4021 aacaaagcct ataaaaaatc tgcccgtgtc gttggtgacg taatcggtaa ataccatccc
     4081 catggtgact cggcggttta tgacacgatc gtccgtatgg cgcagccatt ctcgctgcgt
     4141 tacatgctgg tagacggtca gggtaacttc ggttccatcg acggcgactc tgcggcggca
     4201 atgcgttata cggaaatccg tctggcgaaa attgcccatg aactgatggc cgatctcgaa
     4261 aaagagacgg tcgatttcgt tgataactat gacggcacgg aaaaaattcc ggacgtcatg
     4321 ccaaccaaaa ttcctaacct gctggtgaac ggttcttccg gtatcgccgt aggtatggca
     4381 accaacatcc cgccgcacaa cctgacggaa gtcatcaacg gttgtctggc gtatatcgat
     4441 gatgaagaca tcagcattga agggctgatg gaacacatcc cggggccgga cttcccgacg
     4501 gcggcaatca ttaacggtcg tcgcggtatt gaagaagctt accgtaccgg tcgtggcaag
     4561 gtgtacatcc gcgcccgtgc tgaagtggaa gtcgacgcca aaaccgggcg tgaaaccatt
     4621 atcgtccacg aaattccgta tcaggtaaac aaagcgcgcc tgatcgagaa gattgcggaa
     4681 ctggtaaaag aaaaacgcgt ggaaggcatc agcgcgctgc gtgacgagtc tgacaaagac
     4741 ggtatgcgca tcgtgattga agttaaacgc gatgcggtcg gtgaagttgt gctcaacaac
     4801 ctctactccc agacccagtt gcaggtttct ttcggtatca acatggtggc attgcaccat
     4861 ggtcagccga agatcatgaa cctgaaagat atcatcgcgg cgtttgttcg tcaccgccgt
     4921 gaagtggtga cccgtcgtac tattttcgaa ctgcgtaaag ctcgcgatcg tgctcatatc
     4981 cttgaagcac tagccgtggc gctggcgaac atcgacccga tcatcgaact gatccgtcat
     5041 gcgccgacgc ctgcagaagc gaaaactgcg ctggtggcta atccgtggca gctgggcaac
     5101 gttgccgcga tgctggaacg tgctggcgac gatgctgcgc gtccggaatg gctggagcca
     5161 gagttcggcg tgcgtgatgg tctgtactac ctgaccgaac agcaagctca ggcgattctg
     5221 gatctgcgtt tgcagaaact gaccggcctt gagcacgaaa aactgctcga cgaatacaaa
     5281 gagctgctgg atcagatcgc ggaactgttg cgtattcttg gtagcgccga tcgtctgatg
     5341 gaagtgatcc gtgaagagct ggagctggtt cgtgaacagt tcggtgacaa acgtcgtact
     5401 gaaatcaccg ccaacagcgc agacatcaac ctggaagatc tgatcaccca ggaagatgtg
     5461 gtcgtgacgc tctctcacca gggctatgtt aaatatcagc cgctttctga atacgaagcg
     5521 cagcgtcgtg gcgggaaagg taaatctgcc gcacgtatta aagaagaaga ctttatcgac
     5581 cgactgctgg tggcaaacac gcacgaccat attctgtgct tctccagccg tggtcgcgtc
     5641 tattcgatga aagtttacca gttgccggag gccactcgtg gcgcgcgcgg tcgtccgatc
     5701 gtcaacctgc tgccgctgga gcaggacgaa cgtatcaccg cgatcctgcc agtgaccgag
     5761 tttgaagaag gcgtgaaagt cttcatggcg accgctaacg gtactgtgaa gaaaaccgtc
     5821 ctcaccgaat tcaaccgtct gcgtaccgcc ggtaaagtgg cgatcaaact ggttgaaggc
     5881 gatgagctga tcggcgttga cctgaccagc ggcgaagacg aagtaatgct gttctccgcc
     5941 gaaggtaaag tggtgcgctt taaagagtct tctgtccgtg cgatgggctg caacaccacc
     6001 ggtgttcgcg gtattcgctt aggtgaaggc gataaagtcg tctctctgat cgtgcctcgt
     6061 ggcgatggcg caatcctcac cgcaacgcaa aacggttacg gtaaacgtac cgcagtggcg
     6121 gaatacccaa ccaagtcgcg tgcgacgaaa ggggttatct ccatcaaggt taccgaacgt
     6181 aacggtttag tggttggcgc ggtacaggta gatgactgcg accagatcat gatgatcacc
     6241 gatgccggta cgctggtacg tactcgcgtt tcagaaatca gcatcgtggg ccgtaacacc
     6301 cagggcgtga tcctcatccg tactgcggaa gatgaaaacg tagtgggtct gcaacgtgtt
     6361 gctgaaccgg ttgacgagga agatctcgat accatcgacg gcagtgccgc ggaaggggac
     6421 gatgaaatcg ctccggaagt ggacgttgac gacgagccag aagaagaata attttacttc
     6481 ttcatgccaa aagggagcta tctcccttgt ttgaattgaa aagtccaggc tgcaaagtct
     6541 gggcttttgt cgtattaggg cacggtaaag tttggctgtg cccgtaaaaa atggctggct
     6601 atacacaagg aatgtggcaa tgagtggtga aaaaaagcga aaggctggcg gttctatggt
     6661 cttgtaggtt ttggcgcaat agtactgctt tccgctggtg tctgggcgtt gcaatatgct
     6721 ggcagtgggc cagaaaaaac gttgtcgccg ctggtggtgc acaacaatct gcaaatcgat
     6781 ctcaatgagc cggacctctt tctcgacagc gactctctga gccagcttcc caaagatctc
     6841 ctcaccattc cgtttctcca cgatgttctg agtgaagatt tcgttttcta ttatcagaat
     6901 catgccgatc gtctgggcat tgaaggcagc attcgtcgca ttgtctatga acacgatctc
     6961 acgctgaaag ataaactctt ttcgtcactc ttagatcagc ccgcgcaggc tgcgctatgg
     7021 cacgataaac aaggccatct ttcacattac atggtgctga tccagcgcag tggtttaagc
     7081 aaactgctgg agccattgtt gtttgccgct accagcgaca gccagttaag caaaacggaa
     7141 atcagtagca tcaagataaa tagtgaaact attcctgttt atcagttgcg ctataacggc
     7201 aataacgccc tgatgttcgc gacttatcag gacaagatgc tggtgttttc cagcacggat
     7261 atgttgttta aagatgatca gcaggatacc gaagccacgg cgatcgcagg tgatttgttg
     7321 agcggcaaaa aacgctggca agcaagcttt ggcctggaag agcgtgctgc tgaaaaaacg
     7381 ccagtacgcc agcgcatcgt ggtcagcgcc aggttgctgg gttttggcta ccagcggtta
     7441 atgccttctt ttgctggcgt acgcttcgaa atgggtaacg acggctggca cagttttgtg
     7501 gcgttaaatg atgaatccgc cagcgtagat accagtttcg attttacgcc ggtctggaac
     7561 agtatgcctg ccggggccag cttctgtgtg gtggtgccgt attcacacgg tattgccgaa
     7621 gagatgcttt cgcacatcag ccaggaaaac gacaagctga atggggcgtt agacggtgcc
     7681 gcggggctgt gctggtatga agactcaaaa ttgcaaaccc cgctgtttgt cggtcagttt
     7741 gatggcactg ccgaacaggc gcaattgcca gggaaactgt ttacgcaaaa tattggtgcg
     7801 cacgaaagca aagcgccaga aggtgttttg ccgataagcc agactcagca gggcgaagcg
     7861 caaatctggc gtcgcgaagt gagttcccga tacggtcagt atccgaaagc gcaggcggcg
     7921 caacccgatc aattaatgtc ggattatttt ttccgcgtgt cgctggcgat gcaaaacaaa
     7981 acgctgcttt tctccctcga tgacacgctg gttaataacg ccctgcaaac actgaataaa
     8041 aaccgcccgg caatggtgga tgtaataccc actgatggca tcgttccgct ctatatcaat
     8101 tcacaaggcg tggcgaaact gctgcgtaac gaaacgttga ccagtctgcc gaagaatctc
     8161 gaaccggttt tttataacgc cg
//
LOCUS       DAJTZL010000168         8157 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.168, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000168 DAJTZL010000000
VERSION     DAJTZL010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8157
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.168"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..449)
                     /locus_tag="OO544_003399"
     CDS             complement(<1..449)
                     /locus_tag="OO544_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HCR6768919.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFN"
     gene            complement(566..1612)
                     /gene="ansB"
                     /locus_tag="OO544_003400"
     CDS             complement(566..1612)
                     /gene="ansB"
                     /locus_tag="OO544_003400"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HCR6768920.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            complement(1788..2507)
                     /locus_tag="OO544_003401"
     CDS             complement(1788..2507)
                     /locus_tag="OO544_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HCR6768921.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            complement(2691..3017)
                     /locus_tag="OO544_003402"
     CDS             complement(2691..3017)
                     /locus_tag="OO544_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HCR6768922.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERKLDEVRTSEL
                     FDVWWD"
     gene            complement(3017..3736)
                     /gene="trmB"
                     /locus_tag="OO544_003403"
     CDS             complement(3017..3736)
                     /gene="trmB"
                     /locus_tag="OO544_003403"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HCR6768923.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            3897..4949
                     /gene="mutY"
                     /locus_tag="OO544_003404"
     CDS             3897..4949
                     /gene="mutY"
                     /locus_tag="OO544_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HCR6768924.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAAANNSWSL
                     YPGKKTKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRQWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            4977..5252
                     /locus_tag="OO544_003405"
     CDS             4977..5252
                     /locus_tag="OO544_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HCR6768925.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            5317..6396
                     /gene="mltC"
                     /locus_tag="OO544_003406"
     CDS             5317..6396
                     /gene="mltC"
                     /locus_tag="OO544_003406"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HCR6768926.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLQSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLDGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            6598..7854
                     /gene="nupG"
                     /locus_tag="OO544_003407"
     CDS             6598..7854
                     /gene="nupG"
                     /locus_tag="OO544_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HCR6768927.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(7903..>8157)
                     /locus_tag="OO544_003408"
     CDS             complement(7903..>8157)
                     /locus_tag="OO544_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HCR6768928.1"
                     /translation="IRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQR
                     YFLALEEGVNLLPGFSPELQGVYSETDADGMKRLYGYVLK"
BASE COUNT         1922 a         2160 c         1987 g         2088 t
ORIGIN      
        1 taattgaaat cttcgctatt cacgatcaga tatttattat tcgttttgat atcgtcgacc
       61 acccgcccac gatcgctggc tgccgcctga aaagagtaaa agttcaccga ccagccagac
      121 gcgggcgttc ctttactggt taacatcgcc gagaatttta ccggtttatc tttcgtccag
      181 gtctgttcga gcagttcgta atctgccaac tgaccgaccc aggtatagag atcgtcactg
      241 gaagagacat cgccttccgc tgaccaggtg gcctgattac ccttagcatc aagatttttt
      301 aaggtgatag catcaagctt gagaatgccg tgatactgct ttttgaattg atcttttaag
      361 atattttcag ttggcggttc gccatctgcc caggcattac cagtcatcaa cataagcagt
      421 gccgtgccaa caatccattg ctttttcacc cgaaaaacct cacgcggaat aatttattcc
      481 ggtgattatc attagggtaa tgaaaattgg caatggacgt aagtctgaag tgaaaaagcc
      541 ccggcacgat accggggcga ggtgattagt actgattgaa gatctgctgg atctgctgcg
      601 gatctttggt ttgcgtcaga gccagctgca gcaggacgcg cgctttttgc gggttcagcg
      661 tgccagaggc gacgaagccg tatttcgcat catccacttc agcatcctga gtggtagcac
      721 ccgtcggtac gcgggaagaa cgcactactg cagtgccgtt tttcgcggcg gttgccaggg
      781 tgtcgaacac ggatttatac aggttaccat tacccacacc agcgctaacg atgccatcat
      841 agcccgcatc taccagtgct ttagccggaa gatcggatgc gttagcgtag ttataaacga
      901 tgccgacttt cggcagctca ttcagcttag agacatcgaa tggcgtatcg ctggtgtgct
      961 tacgtgccgg ggtacgttgg tagtcaatct taccgttgtg aatgtatccc agaggaccgt
     1021 agttaacaga cttgaaggtc gctacgtcgg tggtgttggt tttggtgaca tcgcgaccgt
     1081 ccagtacggt gtcgttcatc accaccagca cgccacgatt agcggaggct ttatcagctg
     1141 cggtcactac cgcgttatac aggttgaatg gaccgtctgc gctcatggac gtggacgggc
     1201 gcattgcgcc gaccatcacc accggtttgt cgcatttcac cgtcaggtcg aggaagtaag
     1261 cggtttcttc catcgtgtcg gtaccgtggg taatgacgaa gccgtcggtt ttatcgcagt
     1321 cggtgttaat ttttttcgcc agtgtcagcc agacattatc gttcatgtcc tgggagccga
     1381 tattcactac ctgctcgcct ttaacgttcg caatgtcttt tagttgcggc accgcattaa
     1441 ccagattttc tacgccaact ttacccgctg tgtagttaga tttggttgcg gagtcaccac
     1501 caccggcaat ggtcccgccg gttgctaaaa tggtgatatt gggtaatgcc aatgctgcac
     1561 cactaaaacc cataaccagt gcggcaagtg ccgtcttttt gaaaaactcc atttcattcc
     1621 tccagttacg tgaacgctac gcattatccc ttagctctgt atgggaaatt tgacgttaaa
     1681 caatttacaa cgtgaatata ttttggagat ctacaaagtt agaggcaggt aacaaaacga
     1741 agaattaaac ggcataaaaa agtattatgc cgtcttaaaa tagaggatta ttttaaattc
     1801 ccgaccaggg ctttgcggct atcttccaga gtcacaacgc ggctacaaac atctttgcca
     1861 aactgctgga aatctttttc ctgctttttc cactctgttt ggattgagga ttgcagcccc
     1921 cccaggcttc ccagcacatt ctgtaatggg ttaccgccgc ttttcagcac cgctttcgcg
     1981 cccatttcat taatgctgtc ctgtaaaatt ccacccattg cctgattcac taattgctgg
     2041 ccttcggcgc gaacctgatc aatggcttta tagtgaaacg tcaggccatc gctgcgcgtt
     2101 tcgataatgc ggttcatctg ctctttcagc tgcgcatcaa gtttggtcag acggctgcgc
     2161 attttgctgc tttcgcccat ctcctgaacg ataattttat ccagcgcaat acgggctttt
     2221 tcgacgcggc ttttcgcgcc ttcatcaatc cacggcagcg tgctgcgtag ttcagcctga
     2281 taatccttcg cctgctcgcg ctgggcggca ttcagggaat attgcttacc gttatacatc
     2341 acgttgccgt ctggcgtgat caccagattg ccgttttcgc ccttcacctg cacggtttgc
     2401 gggctgacaa tcacatcgtc acgcggcgtg acgctgcact ggtagtcggc gtgagcggtc
     2461 attgccgtca ctgaaagtgc tgccgccagc agcattttgc gcatcatagt cttccctcaa
     2521 gaaaaaatca ggccagcatt tgctggcccc agattgataa caaagtacgc gttttccatg
     2581 ccggatgcgg cgtaaacacc ttatcccgcc tactcgatcg tgcatctttc caggcctgat
     2641 aagcgtagcg catcaggcag ttttgcattt gtcatcgccc gtatgctttc ttagtcccac
     2701 caaacgtcga aaagttcgct ggtgcgtacc tcatccagtt tgcgctcttc cagccactta
     2761 cgcacaatcg cctgatgttc ttcggtgcat ttgccgattt cctgcatgca gatcagacct
     2821 tcccaggcca gataaccgct gccgtcaaag gccagtttgt tcggttcgat aacctcgtta
     2881 ataaaatcat caacggtttt atcaatctgt tcttccgatg taccttccgg gaatcgccat
     2941 gccaccgaaa atcctaattc ctggaattcg tcgatgtgca tttttttacg cagacgacgg
     3001 ctacggttct ttgccattat ttcaccctct cgaacattaa gtcccatact ccgtgaccaa
     3061 gacgatgacc acgttgttca aatttcgtca ccggacgtga tgccggacgc ggcacgtaat
     3121 cattgctctc tgacaggttt ttataaccgt caatagaaga catcacttca agcatatgtt
     3181 ccgcataagg ttcccagtcg gtcgccatat ggaatacacc ccccagctgc agtttgcttt
     3241 ttaccagttc ggcaaacggc acctgtacga tacggcgttt attatggcgc gctttgtgcc
     3301 acgggtcagg gaaaaagagc tgcaccatgc gcaatgaatt gtcaggaatc attttatgca
     3361 gcacttcaac cgcatcgtga cacatcacgc gcaggttgct taaaccttct tcatgcgcag
     3421 aagccaggca cgcaccaacg cccggtgaat gcacttcaat gccgaggaag tcctgctcag
     3481 ggcgatcttt agccattgcc accagcgacg cccccatgcc aaaaccaatc tcaagcgtca
     3541 ccggcgcttc acggccaaaa agcgcgggga aatccagcat atcttcgctg aactcaacgc
     3601 ccatcaccgg ccagtagttt tccagcgcat gttcctggcc tttggtcagt cgcccctggc
     3661 ggcgcacaaa actacggata cggcgcagtg ggcggccgtt ttcatcaaat tccggtgaaa
     3721 tgacgtcgtt tttcataaag atttagtcgc ttgtgaaagt gttctgaaaa cgggcattat
     3781 ccaaagttag ttgccggatg caagcatgat aaggccgtgg ctgcggaaag ttccggttta
     3841 caccctgccg tcgctgtgct gcaatcttgc ccccaacaac agtgaattcg gtgaccatgc
     3901 aagcgtcgca attttcagcc caggttctgg actggtacga taaatacggg cgaaaaacgc
     3961 tgccctggca aattgacaag acgccctaca aagtatggct ctcagaagtg atgttgcaac
     4021 aaactcaggt tgcgaccgtt atcccctatt ttgaacgctt tatggcgcgc ttcccgacgg
     4081 tgaccgatct cgccaatgcg ccgctcgacg aagttctcca cttgtggacc gggcttggct
     4141 attacgcccg cgcgcgcaat ctgcataaag cggcacaaca agtggcgacc ttacacggcg
     4201 gtaaattccc ggaaaccttt gaagaagtcg cggcgttacc gggcgtcggg cgttccaccg
     4261 caggcgcgat tctctcgctt tctctgggta agcactttcc gattctcgac ggtaacgtca
     4321 aacgcgtgct ggcgcgctgc tatgctgtaa gcggctggcc tgggaaaaaa gaggtcgaga
     4381 ataaattatg gagtttgagc gagcaggtga cgcccgcggt cggcgtggaa cggtttaatc
     4441 aggcgatgat ggatttgggc gcgatgattt gtacgcgctc gaagccgaaa tgttcgctct
     4501 gtccgctaca aaacggatgt attgccgccg ccaataatag ctggtcgctt tatccgggca
     4561 aaaaaacgaa acagacgctg ccggagcgca ccggctactt tttgctgtta cagcacgaag
     4621 atgaagtatt gctggcgcag cgtccgccga gcggattgtg gggcggttta tactgtttcc
     4681 cgcagtttgc cgacgaagaa agtttgcggc agtggctggc gcaacggcag attgctgccg
     4741 ataacctgac gcaactgacc gcgtttcggc ataccttcag ccatttccac ttagatattg
     4801 tgcctatgtg gcttcccgtg tcgtcattca ccggctgcat ggatgaaggc aatgcgctct
     4861 ggtataactt agcgcaaccg ccgtcagttg gcctggcggc tcccgtggag cgtttgttac
     4921 agcagttacg cactggcgcg ccggtttagc gcgtgagtcg ataaagagga tgatttatga
     4981 gcagaacgat tttttgtact ttcctgcaac gtgaagcaga aggtcaggat tttcagctgt
     5041 accccggcga gctgggaaaa cgcatctata acgagatctc caaagaagcc tgggcgcagt
     5101 ggcagcacaa gcaaaccatg ctgattaatg aaaagaaact caacatgatg aatgccgagc
     5161 accgcaagct gcttgagcag gagatggtca acttcctgtt cgagggtaaa gaggtgcata
     5221 tcgagggcta tacgccggaa gataaaaaat aaaaacagtg ccggagcacg cctccggcaa
     5281 cttgcataaa aacaaacaca acacgcaccc ggaatgatga aaaaatatct cgcgctggct
     5341 ttgattgcgc cgttgctcat ctcctgttcg acgaccaaaa aaggcgatac ctataacgaa
     5401 gcctgggtca aagataccaa cggttttgat attctgatgg ggcaatttgc ccacaatatt
     5461 gagaacatct ggggattcaa agaggtggtg atcgccggtc ctaaggacta cgtgaaatac
     5521 accgatcaat atcagacccg cagccacatc aacttcgatg acggtacgat tactatcgaa
     5581 actatcgccg ggacagaacc tgccgcgcat ttgcgccggg caattatcaa aacgttgctg
     5641 atgggtgacg atccgagttc ggtcgatctc tattccgacg ttgatgatat tacgatttcg
     5701 aaagaacctt tcctttacgg tcaggtggtg gacaacaccg ggcagccgat tcgctgggaa
     5761 ggtcgcgcaa gcaacttcgc ggattatctg ctgaaaaacc gtctgcaaag ccgcagtaac
     5821 gggctgcgta tcatctacag cgtcaccatt aacatggtac cgaaccacct tgataaacgt
     5881 gcgcacaaat atctcggcat ggtccgccag gcgtcacgga aatatggcgt tgatgagtcg
     5941 ctgattctgg caattatgca gaccgaatct tcctttaacc cgtatgcggt cagccgttcc
     6001 gatgcgctgg gattaatgca ggtggtacaa catactgccg ggaaagatgt gttccgctcg
     6061 caggggaaat ccggcacgcc gagccgcagt ttcttgtttg atcctgccag caatattgat
     6121 accggcaccg cgtatctggc gatgctgaac aatgtttatc tcgacggaat tgataaccca
     6181 acatcgcggc gttatgccgt catcaccgcc tataacggcg gcgcaggcag cgtgctgcga
     6241 gtcttttcga atgataagat tcaggctgcc aatattatta acaccatgac gccgggcgat
     6301 gtttatcaga cgctgacgac ccgccatccc tctgcggaat ctcgccgtta tctttataaa
     6361 gtgaataccg cgcaaaaatc ctaccgccgc cgataattcc attaaccgcc cctgacgatg
     6421 ctcaggggca aaaatgttat ccacatcaca atttcgtttt gcaaattggg aatgtttgca
     6481 attatttgcc acaggtaaca aaaaaccagt ccgcgaagtt gatagaatcc catcatctcg
     6541 cacggtcaaa tgtgcttttt caaacactca tccgcatcac aatgtgagga aattaacatg
     6601 aatcttaagc tgcagctgaa aatcctctct tttctgcagt tctgtctgtg gggaagttgg
     6661 ctgacgaccc tcggctccta tatgtttgtt accctgaagt ttgacggtgc ttctattggc
     6721 gcagtttata gctcactggg tatcgccgcg gtatttatgc ctgcgctgct ggggattgtg
     6781 gccgacaaat ggttaagtgc gaaatgggta tatgccattt gccacaccat tggcgctatc
     6841 acgctgttca tggcggcaca ggtcacgacg ccggaagcga tgttccttgt gatattgatt
     6901 aactcgtttg cttatatgcc aacgcttggg ttaatcaaca ccatctctta ctatcgcctg
     6961 caaaatgccg ggatggatat cgttactgac ttcccgccaa tccgtatctg gggcaccatc
     7021 ggctttatca tggcaatgtg ggtggtgagc ctgtctggct tcgaattaag ccacatgcag
     7081 ctgtatattg gcgcagcact ttccgccatt ctggttctgt ttaccctgac tctgccgcat
     7141 attccggttg ctaaacagca agcgaatcag agctggacaa ccctgctggg cctcgatgca
     7201 ttcgcgctgt ttaaaaacaa gcgtatggca atcttcttca tcttctcaat gctgctgggc
     7261 gcggaactgc agattaccaa catgttcggt aataccttcc tgcacagctt cgacaaagat
     7321 ccgatgtttg ccagcagctt catcgtgcag catgcgtcaa tcatcatgtc gatttcgcag
     7381 atctctgaaa ccctgttcat tctgaccatc ccgttcttct taagccgcta cggtattaag
     7441 aacgtaatga tgatcagtat tgtggcgtgg atcctgcgtt ttgcgctgtt tgcttacggc
     7501 gacccgactc cgttcggtac tgtactgctg gtactgtcga tgatcgttta cggttgcgca
     7561 ttcgacttct tcaacatctc tggttcggtg tttgtcgaaa aagaagttag cccggcaatt
     7621 cgcgccagtg cacaagggat gttcctgatg atgactaacg gcttcggctg tatcctcggc
     7681 ggcatcgtga gcggtaaagt tgttgagatg tacacccaaa acggcattac cgactggcag
     7741 accgtatggt tgattttcgc tggttattcc gtggttctgg ccttcgcgtt catggcgatg
     7801 ttcaaatata aacacgttcg tgtcccgaca ggcacacaga cggttagcca ctaattacgc
     7861 aaagaaaaac gggtcgccag aaggtgaccc gtttttttat tcttacttca acacataacc
     7921 gtacaatcgt ttcatgccat ccgcatcggt ttcgctatag acaccttgca actccggcga
     7981 aaatcccggc aacaaattca cgccttcttc cagcgcaagg aaataacgtt gaaccgcccc
     8041 accccagact tccccgggaa ccacgcaaag cacgccaggt ggataaggca acgccccttc
     8101 tgccgcaatt cgcccttcgg catcacgaat ccgcaccaac tccacttcac cgcgaat
//
LOCUS       DAJTZL010000169         8104 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.169, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000169 DAJTZL010000000
VERSION     DAJTZL010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8104
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.169"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(101..796)
                     /locus_tag="OO544_003409"
     CDS             complement(101..796)
                     /locus_tag="OO544_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HCR6768929.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAVERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(789..2216)
                     /locus_tag="OO544_003410"
     CDS             complement(789..2216)
                     /locus_tag="OO544_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HCR6768930.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVWFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(2227..2946)
                     /locus_tag="OO544_003411"
     CDS             complement(2227..2946)
                     /locus_tag="OO544_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HCR6768931.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEALPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVVHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
     gene            complement(3474..4328)
                     /gene="rclR"
                     /locus_tag="OO544_003412"
     CDS             complement(3474..4328)
                     /gene="rclR"
                     /locus_tag="OO544_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RclR"
                     /protein_id="HCR6768932.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLQHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSARYFL
                     TSLPETLFLAPVNHSVEYNWLREAIPFLQQESRSAMPGVDALCSQICATFFTLAVREW
                     IAQVNTEKNILSLLLHPRLGAVIQQMLEMPGHAWTVESLASIAHMSRASFAQLFRDVS
                     GTTPLAVLTKLRLQIAAQMFSREMLPVVVIAESVGYASESSFHKAFVREFGCTPGEYR
                     ERVRQLAP"
     gene            4554..5879
                     /locus_tag="OO544_003413"
     CDS             4554..5879
                     /locus_tag="OO544_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridine nucleotide-disulfide oxidoreductase"
                     /protein_id="HCR6768933.1"
                     /translation="MNKYQAVIIGFGKAGKTLAVTLAKAGWRVALIEQSNAMYGGTCI
                     NIGCIPTKTLVHDAQQHTDFVRAIQRKNEVVNFLRNKNFHNLADMPNIDVIDGQAEFI
                     NNHSLRVHRPEGNLEIHGEKIFINTGAQTVVPPIPGITTTPGVYDSTGLLNLKELPGH
                     LGILGGGYIGVEFASMFANFGSKVTILEAASLFLPREDRDIADNIATILRDQGVDIIL
                     NAHVERISHHENQVQVHSEHAQLAVDALLIASGRQPATASLHPENAGIAVNERGAIVV
                     DKQLHTTADNIWAMGDVTGGLQFTYISLDDYRIVRDELLGEGRRSTDDRKNVPYSVFM
                     TPPLSRVGMTEEQARESGADIQVVTLPVAAIPRARVMNDTRGVLKAIVDNKTQRILGA
                     SLLCVDSHEMINIVKMVMDAGLPYSILRDQIFTHPSMSESLNDLFSLVK"
     gene            5988..6224
                     /locus_tag="OO544_003414"
     CDS             5988..6224
                     /locus_tag="OO544_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR6768934.1"
                     /translation="MFKKSVLFATLLSGVMEFSTNADDKIILKHISVSSVSASPTVLE
                     DTIADIARKYNASSWKVTSMRIDNNSTATAVLYK"
     gene            6236..6829
                     /gene="rclC"
                     /locus_tag="OO544_003415"
     CDS             6236..6829
                     /gene="rclC"
                     /locus_tag="OO544_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HCR6768935.1"
                     /translation="MEKYLHLLSRGDKIGLSLIRLSIAIVFMWIGLLKFVPYEADSIT
                     PFVANSPLMSFFYEHPEDYKQYLTHEGEYKPEARAWQSANNTYGFSNGLGVVEVIIAL
                     LVLANPVNRWLGLLGGLMAYTTPLVTLSFLITTPEAWVPALGDAHHGFPYLSGAGRLV
                     LKDTLMLAGAVMIMADSAREILKQRSNESSSTLKTEY"
     gene            complement(6989..7858)
                     /locus_tag="OO544_003416"
     CDS             complement(6989..7858)
                     /locus_tag="OO544_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308365.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCR6768936.1"
                     /translation="MEFSVLSNNLKMPMMGFGVFQVTDKNVCKQSVLNAIRTGYRLID
                     TAAVYGNEDAVGEAVREAISEGLCTRDELFITSKLWGQDMLNQDTAAAGIEASLKKSG
                     LEYFDLYLLHQAMRDYFSVWRALEDAYEEGKLKAIGVSNFYPHVLANFCETVRVKPMV
                     NQVELHPYFAQPEALATMKYYNVQPEAWAPLGGGRHKPFENNLLQSIADAHQKSISQV
                     ILRWNIQRGVVVIPKSTHQQRIEENFAIWDFSLTEKEMAQISSLDLGYVGESVKHFNP
                     EFVRGCLAVKIHD"
BASE COUNT         2193 a         1982 c         1733 g         2196 t
ORIGIN      
        1 agcataacca tgacggaacg gggttaagtc aggttgtcct taacgctatt ttcaacctcg
       61 tatgccttct tcaggtttat gttcagatct catatctctc tcagcaatcc tcaataatca
      121 gatacaccgc tttcaccgga ccatgaaccc cgacgacttt gataagctca atatccgccg
      181 ttgagctggg accactgatg atgttaatac atgacggcat tcgttcaccg gcctgcgctt
      241 tctgatgcaa tttttctgcg agttgcgcta cacgcggcag gattgtgctt ttacgcagga
      301 taaaaagaga agattccggg agcaggctca atgaacgccc gcgctcgacg gcggaaaaaa
      361 gaaccacgcc tcccgattcg gttaaaccat attcagcata cacgacaccc actttagcct
      421 gctctgcctg cgagatattc tcggcacctt tcgccggatc ccaaacaacg gcattgcatt
      481 cctgctgcaa acgttcacta atccccaatt cctccagcct cgtgtcaccg ctaatcacga
      541 ccgactgatc tcccagctct ttacacagac gtattgcagc ttctgccgcc ttcgcctcgc
      601 tggtcagctc acagcgcgtc aacataacat cgctggcaaa ctgaataaac gcgtcacagc
      661 gctgctgttg gttaagttgg gtaagccgct cgttagcata gttgttaagc ggcgcatctt
      721 ctgcttgcgg ttcaagtcgc agcgggcgac ccagtgcctg agcaacgtta ttcaaaaatt
      781 cgctccgatt atccattctt tttctcctgc gcctgatgtt tcttaaacca actacggaaa
      841 ctctctccgt cagcttcagg aagatcgcgt gcttccatcc agtcgctaat cgcgccaaat
      901 ttgagtggtg ttttgccgcc attgataaac cagcttgccg catgagcgcc ggccatcatc
      961 ccgactttcc acaatcctgg atgactattg gcataagcga acatttttat cgcccgttgc
     1021 tctgcttttg cggtgatccc tttttcagcc atcacccgac gatgacgcaa aatcagtttt
     1081 gacagcggaa tacgcaccgg acacacgctg tcacaagcag tgcataaaga gcaggcgtag
     1141 ggtaaatctt taaaatcttt atagccgcca agtagcggag aaatcaccgc accaattggc
     1201 cctggataaa tagagccata tccatgaccg ccaatatggc gatatgccgg acaagtattc
     1261 atacaagccc cgcagcgaat acagcgcagc acatcccgaa attcagaggc cagcacctca
     1321 gaacgcccgt tatcgacaat aaccagatga aactcttcag gaccatcaac gtgccccgct
     1381 tcgcgcggtc ctgtcagcca ggtgttgtat cccgtcaaac gtgcaccaac ggcactgcgc
     1441 gccagcatgg tgatcaatac atctacctcg gcaaacgtgg gggcaatacg ctccattccc
     1501 atcacagcaa tatgcgtttt aggcagcgtg gtacacattc gcgcattacc ttcattggtc
     1561 accaggcata ccgaacctgt ctctgccacc gcgaaattac agccggtaat acctatttca
     1621 gcactgagga aatcttcgcg gattttttgc cggatgaata aggtcatcgc ttcaggcgtt
     1681 tccggcccct catagcccag acgttcgtgt agcactcgac ggatctgatg gcgatcttta
     1741 tgaattgccg ggaccacaac atgagatggc ggatcttgat ccagctggag aatatattca
     1801 cccagatcgg tttcaatcac ctgaatgcca gcatcctgca acacatgatt gacaccaatc
     1861 tcttcggtca ccatcgattt agatttcacc accttccggg catttttgcg ttgggcaacc
     1921 tgtaaaatgt agcgggtagc gtcttctttg gtttttgcaa accagacgtg accgccgttt
     1981 tgcgtcactt tttctgagag ctggtacaga taagcgtcga gattactcag aacatgatca
     2041 cgtatctggg cggcccgatc gcgccactcc tcccagtgcc ccaattcatc gaccattttt
     2101 tgccgatttg ccccaatacg ctgctgcgcg tttgccaccg ctttgcgcat gatcggatct
     2161 tcaatttgct gacggatgcg tgtcttaaaa tctgtattac tggttttgat cgacatcttt
     2221 atatcctcag cggctcatca acacttcagc aatatgcatc actttgactt tctgcccttc
     2281 ccgttgtaat cgcccactga tgttcagcag gcaactcacg tcagcaccaa ttaaatactc
     2341 agggcggact tccatcaggt gcacaacctt ttctttcacc atctcgccgg atatttcggc
     2401 cattttgacc gagaacgtgc cgccaaatcc gcagcaggta tcctgttcag caaaggtcaa
     2461 cagctccagt ccacgcacat ttttcagcag cgtaagtggc tcgtccttca ctcccagctt
     2521 acgggccagg ctacaagatg ggtgatacac agctctccct tgcaaactgg cacctacatc
     2581 gactacccct aatttattaa caataaaaga ggtgagatcc tgcatacgcg cggcaacctt
     2641 ttcggcacgt gatgcccatt caggttcatc cgccagatac gtcgggtaac tttttacggc
     2701 ataggtgcaa gagccagccg gtgaaataat gggatcgtcg ttatcctcca gtgcggcgat
     2761 cagatttttc atccctggaa gcgcttcttt gatataaccg ctattgatcg caggctgacc
     2821 gcagcatccc tgtttctccg ggaaatttac gcgacagccg agtttttcca gtagcagcac
     2881 ggagtctcgt gccattcttg atttcagggc gtcaccaata caggtgacaa agaaattgac
     2941 attcacaact aatactccat tacttcatgc ccatttatgc ccgatggttc ccctccttcc
     3001 gggggatcaa tcatcaataa atatggcgtt cttttttaat tggctagcaa cataccaccc
     3061 ctcatttgtg tgacaaatga taatctttca tccaaacttc aaatgcaata actaatttta
     3121 tttctttcgg catcgctcac attttatggg gaaatcgacg ttcatttttg gcataagaaa
     3181 tccatttaaa aaggctacat cccttatacc tattcagagc tacttattca ggggttattg
     3241 agatatacac tgatggtgta caaaaaaatc accgacaata atcacgtatc aagtaatagt
     3301 cggggtttta tttaattgaa gcaatttagt ttgcattttt atttaaaaac tgagctagag
     3361 tccatcagaa aaaaatatat aaagcccaaa ccaccgttgg aaatagaaat agttgaaagg
     3421 ctcacaactc ccggaatgcg gattatcttt gccctgattt ctggcggttt tattcagggt
     3481 gcaagctgtc tgaccctttc ccgatattct cccggagtac aaccaaactc gcggacaaac
     3541 gccttgtgaa aagatgattc actggcatag cctactgact cagcgatcac cacaacaggg
     3601 agcatttccc gggaaaacat ctgggccgct atttgtagac gcaactttgt taatacagcc
     3661 agcggcgtgg ttccggaaac atcacggaaa agctgggcaa aacttgcccg agacatgtgg
     3721 gcgatgctgg ccagcgattc gacggtccag gcgtgtcctg gcatttccag catttgctgt
     3781 attaccgcac caagacgtgg atgcagaagc aaactgagaa tgtttttctc tgtattaacc
     3841 tgtgcaatcc actcacgcac cgcgagggta aagaatgtag cgcagatctg gctacacagt
     3901 gcatccactc ccggcatcgc cgatctggat tcctgttgta aaaacgggat cgcctccctc
     3961 agccagttat attccacgct gtggttaacc ggtgctaaaa acagcgtttc cggcaaagac
     4021 gttaaaaaat aacgcgccga atgttgcaac cgaagagtgc cgcagacaat acaggtcgat
     4081 tcattatcga catgactcag gcgatgagct gaattttgtg gtagcaggac cacatttccc
     4141 gggcgtaatg taaaaatctc tcccgtcggc atttccagct tcgccgctcc ttgcgttaac
     4201 gcatgccaac gaataaccga taattccccg gcaccatgtt gaagctgcca gtcacttcct
     4261 aacacgcaat tcttatcgat cgttccttgt ggagcgttaa gcatcaacaa acggctaagg
     4321 gcatccatat cagactcctg agtggttttt gattacaaac accaacaatc ttagtaaata
     4381 actaatctaa taccatcaaa tggttaatca tgatttttgt tttgtgattc tgattaagaa
     4441 aaagaaaata ccagacgata agacaaaata tcaagcgttg tgaagaaatg gtatttgctc
     4501 ttttgcgtct atagtcatga tgtcaaatga acgtgtttcg acaggaaatc atcatgaata
     4561 aatatcaggc agttattatt ggttttggca aggctggaaa aacattagcc gtcacgctgg
     4621 caaaagcagg ttggcgagtg gctctcatcg aacaatcaaa tgcaatgtat ggcgggacct
     4681 gtattaatat cggctgcatc ccaaccaaaa cattggttca tgacgcacag cagcacacag
     4741 attttgtccg tgccatacag cgtaaaaatg aagtggttaa ttttttacgt aataagaatt
     4801 ttcataatct tgcggatatg cccaatatcg acgtgatcga cggccaggcg gagtttatca
     4861 ataatcatag cctgcgtgtt catcggcctg agggaaatct ggaaattcat ggcgagaaaa
     4921 tttttattaa taccggtgca caaaccgtgg ttccgccaat tcctggaatt accaccacgc
     4981 ctggtgtata tgacagcacc ggattactta atctaaaaga attgcctggg catttaggta
     5041 ttttgggcgg cggatatatt ggcgttgagt tcgcctctat gttcgctaat tttggcagca
     5101 aggtaaccat tttagaagca gcttcgctgt ttttgcctcg ggaagatcgg gatattgctg
     5161 ataatatcgc gacgatttta cgcgatcagg gcgtcgatat tatcctcaat gcccatgtgg
     5221 agcgaatcag tcaccatgaa aatcaagtgc aagtgcatag cgagcacgcc caactggcgg
     5281 tggatgcact gttaatagct tccggtcgtc aaccggctac cgcttcgtta catccagaaa
     5341 atgccggtat cgcagtaaac gagcgcgggg caattgtcgt tgacaagcaa ttacatacca
     5401 ccgcagacaa tatttgggcg atgggagatg ttaccggcgg gctgcaattt acttatattt
     5461 cactggatga ttaccgcatt gtgcgtgatg agttgctggg agaaggcaga cgtagtactg
     5521 atgatcggaa aaatgtgcct tattccgtat tcatgacacc gccactgtcc agggttggta
     5581 tgacagaaga acaggccaga gagagtggtg ctgatattca ggtggtgaca ttgcctgtag
     5641 ctgcaattcc gcgtgccaga gtgatgaatg atactcgtgg ggtattaaaa gcgattgttg
     5701 ataataaaac ccaacgtata ttaggggcat cactgctgtg tgttgactcc cacgagatga
     5761 tcaatatagt gaaaatggtg atggatgccg ggctgcctta tagcatatta cgcgatcaga
     5821 tatttactca tccgtcgatg agcgaatcac tcaatgatct attttcatta gtcaaataaa
     5881 ctcaaaatca gacgccagaa caaatattct ggcgtctcag agaaaagaat cttattaatt
     5941 ctctgttact ctataacttc ttaatcactt cattgatggt attttatatg tttaaaaaat
     6001 ctgttttatt tgcaacacta ttatctggcg ttatggaatt ttccaccaat gcagatgata
     6061 aaataattct gaaacatatc agcgtctcgt cagtatcagc atcaccgaca gttctggagg
     6121 ataccattgc tgatatagcc agaaaatata atgcttcatc ctggaaagtc acatcgatgc
     6181 gaattgataa taattcaacc gcaacagcag tattgtataa ataaggatgt tcacaatgga
     6241 aaaatacctg cacctgttaa gtcggggaga taaaattggc ctgtcattga ttcgtctgag
     6301 tattgcgatt gtttttatgt ggattgggtt attaaagttt gtcccttacg aggcagacag
     6361 cattacacca ttcgtcgcaa acagtccact aatgtcgttc ttttatgaac acccggaaga
     6421 ctataaacag tatctgactc acgaaggcga atacaaacca gaagcaaggg catggcaatc
     6481 ggccaataat acctatggtt tttccaacgg tcttggcgtc gtggaggtga ttattgctct
     6541 gctggttttg gctaatcctg tcaatcgctg gttaggttta ttgggaggac tgatggcata
     6601 taccacgccg ctggtaacac tctcattttt aatcaccacc ccggaggcat gggtacccgc
     6661 attgggtgac gctcatcatg gtttccctta tttatccggt gctggtcgct tggtattgaa
     6721 agatactctg atgctggcag gtgctgtcat gataatggca gattcggcgc gggaaattct
     6781 taaacaacgc agtaatgaat ccagttcaac gttaaaaact gaatattgat aatccgaaca
     6841 ctgtctgttg ttcacttttt caggcgtcag gccccctttc tgatgatgaa gcctgacgcc
     6901 atattaccgg agatatgaca gagtgaagat caatatcact cccctgttaa aagattcttg
     6961 tcgtctcagg taaaatatat gattaatatc aatcatgtat ttttacagca agacaaccac
     7021 gaacaaattc aggattaaaa tgttttaccg actccccaac ataacccaaa tcaagcgaac
     7081 taatttgtgc catttctttc tctgtcagtg agaaatccca gatagcaaaa ttttcttcga
     7141 tacgctgttg atgtgtcgat ttcggaataa cgaccactcc ccgttgaata ttccaacgca
     7201 gaatgacttg agaaatcgat ttttgatggg catctgcaat actctgaagc agattatttt
     7261 caaagggttt atgtcgtcca ccacctaacg gagcccatgc ttcaggctgc acgttataat
     7321 acttcatggt tgccagcgcc tctggttggg caaaataggg atgcaactcg acctggttga
     7381 ccatcggttt aactcttacc gtttcacaaa agttcgccag aacatgagga tagaaattgg
     7441 aaaccccaat tgcttttaat ttgccctctt cataggcatc ttcgagtgca cgccacacac
     7501 tgaaataatc gcgcatagcc tggtgcaata aatagaggtc gaagtactct agtcccgatt
     7561 tttttaatga tgcttcaata cctgctgctg ccgtatcctg attcaacata tcctgccccc
     7621 acaatttaga cgttataaat aactcatctc gggtacataa accttcagat atagcttcac
     7681 gaacggcttc accgacggca tcttcattac cgtataccgc tgcagtgtcg atgagtcgat
     7741 aacccgtgcg gatagcgttc aacactgact gtttgcacac gtttttatcg gtaacctgaa
     7801 aaacaccaaa tcccatcatc ggcattttca gattgttact taatacggaa aattccactg
     7861 tcagtccctc cattaggcat gaacaatgag tctacgttaa aacataacct caaattagta
     7921 tgtggatttt gatatcactt atgcaaaaaa ttcattaata atgtaggact gaaacctctc
     7981 tattttcggg gacaacgaag cagacgctac cagtgctttt gccttcgacc ttgctgtttt
     8041 tgatacactt agggccttgg gcataacgaa aatgtgcgat atcacgaaga attaatggag
     8101 aatg
//
LOCUS       DAJTZL010000170         7968 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.170, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000170 DAJTZL010000000
VERSION     DAJTZL010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7968
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.170"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..239
                     /locus_tag="OO544_003417"
     CDS             <1..239
                     /locus_tag="OO544_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HCR6768937.1"
                     /translation="LLAHTEINVNQTNHVGWTPLLEAIVLNDGGIKQQAIVQLLLEHG
                     ASPHLTDKYGKTPLELARERGFEEIAQLLIAAGA"
     gene            279..1142
                     /locus_tag="OO544_003418"
     CDS             279..1142
                     /locus_tag="OO544_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HCR6768938.1"
                     /translation="MWALTADADFLAQRGQGQVEQVFARAVNIALPARQQLLTLLCEE
                     YDNAPNSCRLALTHFDDLFRHGDKVQFDDQGITVGQHLHIEMSRCRRWLSPTLQMTAV
                     NFHLIAWLQWHDIIHQHLGENETLFNYRGDNPFYQALNKELHIKRRAVIQTVNDKQNI
                     AAAVASMIGLGIGLTPSADDYLTGLALILFIPGHPAEKYKEEFYRGLLRGRNNTTLLS
                     AITLEAALQQRCRENIHRFIHNIIYGVPGNSTQAIEKIKHIGSSSGCDMLYGMADGCA
                     LSQTYGGNYVS"
     gene            1132..2679
                     /gene="fdrA"
                     /locus_tag="OO544_003419"
     CDS             1132..2679
                     /gene="fdrA"
                     /locus_tag="OO544_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HCR6768939.1"
                     /translation="MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNK
                     GVLKNLGLLTPTLEQAKNGDLMIVINGKSGADNEQLLVEIEELFNTKAQSGSHEARYA
                     TIASAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAH
                     EKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFGGGVSQ
                     LIGTGGRDLSEKIGGLMMLDAIGMLENDPQTEIIALISKPPAPAVARKVLERARACRK
                     PVVVCFLGRVETPVDEQGLQFARGSKEAVLKAVMLSGVKQENLDLHTLNQPLIADVRA
                     RLQPQQKYIRGLFCGGTLCDETMFAVMEKHGDVYSNIQPDPEFRLQDINRSIKHTFLD
                     FGDDDFTNGKPHPMIDPTNRISRLIEEARDPEVAVIVMDFVLGFGSHEDPVGSTIEAI
                     KEAKAIAAAEGRELIILAYVLGTDLDTPSLEQQSQMLLDAGVILASSSTNTGLLAREF
                     ICKGEEA"
     gene            2679..4037
                     /locus_tag="OO544_003420"
     CDS             2679..4037
                     /locus_tag="OO544_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HCR6768940.1"
                     /translation="MSQSLFSQPLNVINVGIAMFSDDLKKQHVEVTQLDWTPPGQGNM
                     QVVQALERIIQSHPVLIGFDQAINVVPGMTPKTILHAGPPITWEKMCGAMKGAVTGAL
                     VFEGLAKDLDEAAELAASGEITFSPCHEHDCVGSMAGVTSASMFMHIVKNKTYGNIAY
                     TNMSEQMAKILRMGANDQSVIDRLNWMRDVQGPMLRDAMKIIGEIDLRLMLAQALHMG
                     DECHNRNNAGTTLLIQALTPGIIQAGYSVEQQREVFEFVASSDYFSGPTWMAMCKAAM
                     DAAHGIEYSTVVTTMARNGVEFGLRVSGLPGQWFTGPAQQVIGPMFAGYKPEDSGLDI
                     GDSAITETYGIGGFAMATAPAIVALVGGTVEEAIDFSRQMREITLGENPNVTIPLLGF
                     MGVPSAIDITRVGSSGILPVINTAIAHKDAGVGMIGAGIVHPPFACFEKAILGWCERY
                     GV"
     gene            4180..5130
                     /locus_tag="OO544_003421"
     CDS             4180..5130
                     /locus_tag="OO544_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase family protein"
                     /protein_id="HCR6768941.1"
                     /translation="MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADMVLEMLASD
                     YDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLA
                     RHGEKKAVTVVTQVEVDKNDPGFAHPTKPIGAFFSESQRDKLQKANPDWCFVEDAGRG
                     YRRVVASPEPKRIVEAPAIKALIQQGFVVIGAGGGGIPVVRTEAGDYQSVDAVIDKDL
                     STALLAREIHADILVITTDVEKVCIHFGKPQQQALDRVDIATMTRYMQEGHFSPGSML
                     PKIIASLTFLEQGGKEVIITTPECLPAALRGETGTHIIKT"
     gene            5140..6522
                     /locus_tag="OO544_003422"
     CDS             5140..6522
                     /locus_tag="OO544_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HCR6768942.1"
                     /translation="MKESNSRREFLSQSGKMVTAAALFGTYVPLAHAAVAGTLNCEAN
                     NTMKITDPHYYLDNVLLETGFDYENGVAVQTRTARQTVEIQDGKIVALRENKQHPDAT
                     LPHYDAGGKLMLPTTRDMHIHLDKTFYGGPWRSLNRPAGTTIQDMIKLKQKMLPELQP
                     YTQERAEKLIDLLQSKGTTIARSHCNIEPVSGLKNLQNLQAVLARRQAGFECEIVAFP
                     QHGLLLSKSEPLMREAMQAGAHYVGGLDPTSVDGAMEKSLDTMFQIALDYDKGVDIHL
                     HETTPSGVAAINYMVETVEKTPQLKGKLTISHAFALATLNEQQVDALANRMAAQQISI
                     ASTVPIGTLHMPLKQLHDKGVKVMTGTDSVIDHWSPYGLGDMLEKANLYAQLYIRPNE
                     QNLSRSLFLATGDVLPLNEKGERVWPKAQDDASFVLVDASCSAEAVARISPRTATFHK
                     GQLVWGSVAG"
     gene            6899..7948
                     /gene="yahK"
                     /locus_tag="OO544_003423"
     CDS             6899..7948
                     /gene="yahK"
                     /locus_tag="OO544_003423"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase YahK"
                     /protein_id="HCR6768943.1"
                     /translation="MKIKAVGAYSAKQPLEPMDITRREPGPNDVKIEIAYCGVCHSDL
                     HQVRSEWAGTVYPCVPGHEIVGRVVAVGDQVEKHAPGDLVGVGCIVDSCKHCEECEDG
                     LENYCDHMIGTYNSPTPDEPGHTLGGYSQQIVVHERYVLRIRHPQEQLAAVAPLLCAG
                     ITTYSPLRHWQAGPGKKVGVVGIGGLGHMGIKLAHAMGAHVVAFTTSEAKREAEKALG
                     ADEVVNSRNADEMAAHLKSFDFILNTVAAPHNLDDFTTLLKRDGTMTLVGAPATPHKS
                     PEVFNLIMKRRAIADSMIGGIPETQEMLDFCAEHGIVADIEMIRADQINEAYERMLRG
                     DVKYRFVIDNRTLTD"
BASE COUNT         1919 a         2018 c         2308 g         1723 t
ORIGIN      
        1 agcttttggc gcatacggag attaacgtta accagaccaa ccatgtaggc tggacgccgc
       61 tactggaagc gattgtgctt aatgatggtg gtattaaaca gcaggcgatt gtgcagttat
      121 tgctggaaca cggtgccagc ccgcatctga ccgataaata tggcaaaacg ccactggaac
      181 tggcgcggga acggggcttt gaagagattg cgcagttact gattgccgca ggtgcataaa
      241 ccgggaggct ggctatcaac acaccagaaa aacggtgtgt gtgggcgcta actgcggatg
      301 cggattttct ggcgcagcgg gggcaaggac aggttgaaca ggtctttgcc agagcggtaa
      361 atatcgcact cccggctcgc cagcagttgc tgacgctgct ttgtgaagag tacgacaatg
      421 cgccaaacag ttgtcggttg gcactcactc actttgatga tctgttccgg catggtgata
      481 aggttcagtt tgacgatcaa ggtattacgg ttggtcaaca tcttcatata gagatgagtc
      541 gttgtcggcg ttggctgtcc ccaaccttgc aaatgaccgc tgtgaatttt caccttatcg
      601 cctggctaca gtggcacgac attattcatc agcacctggg ggaaaatgaa accctgttta
      661 attatcgcgg cgataatccg ttttatcagg cgttaaataa agaattacat attaaacgac
      721 gggcagttat tcagacagta aacgataagc aaaatatcgc cgcagcggtc gccagtatga
      781 ttgggttagg gattggcctt acgccatcag ccgatgatta tttaacaggt ctggcgctta
      841 ttttatttat tcccgggcat ccggcagaaa aatacaaaga ggaattttat cgcggtctgc
      901 tacgcggcag aaataacacc acgttattaa gtgccataac gctggaagcc gcattacaac
      961 aacgctgccg ggaaaatatt catcgcttta ttcacaacat tatttatggc gtccctggga
     1021 attcaactca ggcaatagaa aaaattaaac atattggctc cagttccggc tgcgacatgc
     1081 tgtatggcat ggccgatggt tgtgcgctga gccaaaccta cggagggaat tatgtcagtt
     1141 aaaatagtca ttaaaccgaa tacctatttt gattctgtct cgctgatgtc tatctccacg
     1201 cgtgcaaata aactcgacgg cgtcgagcag gcatttgtgg cgatggcgac cgaaatgaac
     1261 aaaggtgtgc tgaaaaattt aggactgctg acgccgacgc tggaacaggc gaaaaacggc
     1321 gacctgatga ttgtcatcaa tggtaaatcg ggtgcggaca acgagcagtt gctggtggag
     1381 attgaagaac tgttcaacac caaagcgcaa agcggctcgc acgaggcgcg ttacgccact
     1441 attgccagcg ccaaaaagca tatcccggaa agtaacctgg cggtgatttc ggtcaacggt
     1501 ctgtttgccg ctcgcgaagc gcgtcaggcg ctgcaaaacg atctcaacgt gatgctgttt
     1561 tccgataacg tctcagttga agatgaactg gcgctcaagc agttggccca cgaaaaaggg
     1621 ctgctgatga tggggccgga ctgcggcacg gcgattatta acggcgcggc gctctgtttt
     1681 ggtaacgccg tgcgtcgcgg caatatcggt attgttggcg catccggcac cggcagccag
     1741 gagctgagcg tccgcattca tgaatttggt ggcggtgttt cacaactgat tggcaccggc
     1801 gggcgcgacc tgagcgagaa aatcggcggc ctgatgatgc tcgacgccat cgggatgctg
     1861 gaaaacgatc cgcaaactga aattatcgca ctgatttcaa aacctcccgc gcctgcggtg
     1921 gcccgcaaag tgctggaacg cgcacgtgcc tgccgcaagc cggtggtggt ctgcttcctc
     1981 ggtcgcgttg aaacgccggt ggatgagcag gggctacagt ttgcccgcgg cagcaaagag
     2041 gcggtgctaa aagcggtgat gctctccggc gtgaaacagg aaaatctcga cctgcatacg
     2101 cttaaccagc cgttgattgc tgatgtgcgt gcgcgtctgc aaccgcagca gaaatacatt
     2161 cgtggcctgt tctgcggcgg cacgctgtgc gacgaaacca tgttcgcggt gatggaaaaa
     2221 catggcgatg tctacagcaa cattcagccc gatccggaat tccgcctgca agatatcaac
     2281 cgcagcatca aacacacctt cctcgacttt ggcgatgacg acttcacgaa cggcaagccg
     2341 catccaatga ttgaccccac caaccgcatc agtcgcttga tcgaagaggc gcgcgatcca
     2401 gaagtggcgg tgatcgtgat ggattttgtg ctcggatttg gatcgcatga agatccggtc
     2461 ggctccacca tcgaggcgat caaagaagcg aaagcgatcg ccgctgccga aggacgcgag
     2521 ttgatcattc tcgcctatgt gctgggtacc gatcttgata cgccatcgtt agaacaacaa
     2581 agccagatgc tgcttgatgc cggagtgatt ctggcgagca gcagcaccaa taccggattg
     2641 ctggcgcgtg aatttatctg caaaggggag gaagcctgat gagccagtca ctgtttagcc
     2701 aaccattgaa cgttattaac gtcggcatcg ccatgtttag cgatgacctg aaaaagcagc
     2761 atgtagaagt gactcaactc gactggacgc cgccggggca gggcaatatg caggtggtgc
     2821 aggcgctgga gcgcattatc cagtcgcatc cggtgctgat tggttttgat caggcgatta
     2881 acgtggtgcc gggcatgacg ccgaaaacca ttcttcacgc cgggccgccg atcacctggg
     2941 aaaaaatgtg cggcgcgatg aaaggcgcgg tcaccggagc gctggtgttc gaaggactgg
     3001 cgaaagatct cgacgaggcg gctgaactgg cggcctccgg ggagatcacc ttctcgccgt
     3061 gccacgaaca cgactgtgtg ggatcgatgg cgggcgttac ctcggcctcg atgtttatgc
     3121 acatcgtgaa aaacaaaacc tacggcaaca tcgcttacac caatatgagc gagcagatgg
     3181 cgaagatttt gcgtatgggt gccaacgatc agagcgtgat cgaccgcctc aactggatgc
     3241 gcgatgtgca ggggccaatg ctgcgcgacg cgatgaaaat tatcggtgag atcgatctgc
     3301 gcttaatgct ggcgcaggcg ctgcatatgg gcgatgagtg ccataaccgc aataacgccg
     3361 ggacgacgct gctgattcag gcactgacgc cggggatcat tcaggcaggc tattccgtcg
     3421 agcaacagcg cgaagtgttc gagtttgtcg ccagcagcga ctacttctcc ggcccgacgt
     3481 ggatggcgat gtgtaaagcg gcgatggatg cggcgcacgg catcgaatac agcaccgtgg
     3541 tcaccaccat ggcgcgtaac ggcgtcgagt tcggcctgcg ggtcagcggc ctgccggggc
     3601 aatggtttac cggcccggcg cagcaggtga tcggcccgat gtttgccggt tataagccgg
     3661 aagattcggg gctggatatc ggcgacagcg ccatcaccga aacctacggt attggtggat
     3721 ttgctatggc gaccgcgcct gcaatcgtcg cgctggtggg cggcacggtg gaagaggcta
     3781 tcgatttctc ccgtcagatg cgtgaaatca ctctcggtga aaaccccaac gtcaccattc
     3841 cgctgctcgg ttttatgggc gtgccgtcgg caatcgacat cacccgcgtg ggtagcagcg
     3901 gcattctgcc ggtgatcaac accgccatcg cccataaaga tgcgggcgtc ggcatgattg
     3961 gcgcgggcat tgtgcatcca ccattcgctt gcttcgagaa agccattctc ggctggtgcg
     4021 aacgttacgg cgtctgactt acatgtttat gccggatgcg gcgtgaacgc cttatccggc
     4081 ctacggatgg cccgagaatt gtaggcctga taagcgtagc gcatcaggca gtctggcgtt
     4141 ggtcataacc tcatcaccct ctgtagcgga cataacaaca tgaaagagct tgtggtcgtt
     4201 gccattggtg gcaacagcat tatcaaagat aacgccagcc agtcgattga gcatcaggcg
     4261 gaggcggtga aagccgtggc cgatatggtg ctggaaatgc tggcttccga ttacgacatt
     4321 gtgctgaccc acggtaacgg gccgcaggtc gggctggatt tacgccgtgc ggagattgcc
     4381 cacgagcgcg aagggctgcc cttaacgccg ctggcgaact gtgtggcaga tacgcagggc
     4441 ggcatcggct atctgatcca acaggcgctg aataaccgac tggcgcgtca cggcgagaaa
     4501 aaagccgtca ccgtggtaac tcaggtggaa gtggataaaa acgatccagg ttttgcccat
     4561 cccaccaagc ccattggtgc attcttcagt gaaagccagc gtgacaaact acaaaaggca
     4621 aaccctgact ggtgttttgt tgaagatgcc ggacggggct atcgccgcgt ggtcgcctcg
     4681 ccggaaccga aacgtattgt cgaagcaccc gccattaagg cgctgatcca acaaggtttt
     4741 gtcgtaattg gcgcgggcgg cggtggaatt ccggtagtgc gtactgaagc gggggattac
     4801 caaagcgtgg acgcggttat tgacaaagat ctctctaccg cgctgctggc ccgtgaaatt
     4861 cacgccgaca ttctggtgat caccaccgac gtcgaaaaag tctgtatcca ctttggtaaa
     4921 ccgcagcagc aggcgctgga tcgggtggat attgccacca tgactcgcta tatgcaggaa
     4981 gggcattttt ctcccggcag catgttgcca aaaatcatcg ccagcctgac gttcctggag
     5041 cagggcggca aagaagtgat tatcaccacg ccggaatgcc tgcctgcggc gctgcgcggt
     5101 gaaacgggca cccatattat taaaacgtaa ggacgtagga tgaaagaaag caatagccgc
     5161 cgtgaatttc tgagccagag cggtaagatg gtcaccgccg ccgcgctgtt tggtacctat
     5221 gtgccgctcg cccatgcggc ggtagctggc accctaaact gcgaagcgaa caacaccatg
     5281 aaaatcactg acccgcatta ctatctcgat aacgtgctgc tggaaaccgg ttttgactac
     5341 gaaaacggtg tggcggtgca aacccgcacg gcgcgccaga ccgtggagat tcaggacggc
     5401 aaaattgtcg ccctgcgcga gaacaagcag catccggacg ccacgctgcc gcactatgac
     5461 gctggcggta agttgatgct acccaccact cgcgacatgc atattcatct cgacaaaacc
     5521 ttctacggcg ggccgtggcg ctcgctcaat cgtccggcag gcaccactat ccaggacatg
     5581 atcaaactca agcagaaaat gctgccggag ttacaaccgt atacgcagga gcgggcggaa
     5641 aaactgatcg atctattgca gtcgaaaggc accaccattg cccgcagcca ctgcaatatc
     5701 gaaccggttt ccggcctaaa aaatctgcaa aatttgcagg cggtgctggc gcggcgtcag
     5761 gcgggcttcg agtgtgaaat cgtcgccttc ccgcagcacg gtttgctgct gtcgaaatcg
     5821 gaacccttaa tgcgcgaagc gatgcaggcg ggtgcgcatt acgtcggcgg gctggacccg
     5881 accagtgtcg atggcgcgat ggaaaaatcc ctcgacacca tgtttcagat tgcgctggac
     5941 tacgacaaag gtgtcgatat tcacctgcac gaaaccactc cgtcgggcgt ggcagccatc
     6001 aattatatgg ttgaaacggt agagaaaacg ccgcaactga aaggtaagct gaccatcagc
     6061 cacgcctttg cgctggctac gctcaacgaa caacaggtcg atgcgctggc gaatcggatg
     6121 gcggcgcagc aaatttctat cgcctcgacg gtgccgattg gcacgctgca tatgccgctc
     6181 aaacagttgc acgacaaagg cgtaaaagtg atgactggca ctgacagcgt tatcgaccac
     6241 tggtcgcctt atggtctggg cgacatgctg gaaaaagcca atctctacgc gcagctctat
     6301 attcgtccta acgaacagaa cctctcccgt tcgctgtttc tagccactgg cgatgtattg
     6361 ccgctgaatg aaaaaggcga gcgtgtatgg ccaaaagcgc aggatgatgc cagctttgtg
     6421 ctggtggacg cctcctgttc cgccgaggcg gtggcgcgta tctcgccgag aaccgcaacg
     6481 ttccataaag ggcaactggt gtgggggagt gtggcaggtt gatgcgcatt tgaaacgcct
     6541 gcaacggtga attgtaagag atttaagccc cagggagtgg ggcgacctca atatgatagt
     6601 gtcggcagaa atggacatta tctttgagga atatgccctt attgacgttg tgtagataac
     6661 tatttgtcta cgtgaaaaga tcatcagttt tgccgatttt agtcttttac ggatagcaaa
     6721 tatcacactt ataggcgctc gccttatggg gaacgttgct gctctgtttc attcaggttc
     6781 gcggcatgtt ggggctggat cgggtggttt aaatattggc tatattcaat ggacgcgttt
     6841 tgccgcgatg acatatcagg cgtcgccaaa tacacatagc taatcaggag taaacacaat
     6901 gaagatcaaa gctgttggtg catattccgc taaacaacca cttgaaccga tggatatcac
     6961 acggcgtgaa ccgggaccga atgatgtcaa aatcgaaatc gcttactgtg gcgtctgcca
     7021 ttccgatctc caccaggtcc gttccgagtg ggcggggacg gtttacccct gcgtaccggg
     7081 tcatgaaatt gtggggcgtg tggtagccgt tggtgatcag gtagaaaaac atgcgccggg
     7141 cgatctggtc ggtgtcggct gcattgtcga cagttgtaaa cattgcgaag agtgtgagga
     7201 cgggctggaa aactactgtg atcacatgat cggcacctat aactcgccga cgccggacga
     7261 accgggccat actctgggcg gctactcaca acagatcgtc gttcatgagc gatatgttct
     7321 gcgtattcgt cacccgcaag agcagctggc ggcggtggct cctttgttgt gtgcagggat
     7381 caccacgtac tcgccgctac gtcactggca ggccgggccg ggtaaaaaag tgggcgtggt
     7441 cggcatcggc ggtctgggac atatggggat taagctggcc cacgcgatgg gggcacatgt
     7501 ggtggcattt accacttctg aggcaaaacg cgaagcggaa aaagccctgg gggccgatga
     7561 agttgttaac tcacgcaatg ccgatgagat ggcggctcat ctgaagagtt tcgatttcat
     7621 tttgaataca gtagctgcgc cacataatct cgacgatttt accaccttgc tgaagcgtga
     7681 tggcaccatg acgctggttg gtgcgcctgc gacaccgcat aaatcgccgg aagttttcaa
     7741 cctgatcatg aaacgccgtg cgatagccga ttctatgatt ggcggcattc cagaaactca
     7801 ggagatgctc gatttttgcg ccgaacatgg catcgtggct gatatagaga tgattcgggc
     7861 cgatcaaatt aatgaagcct atgagcgaat gctgcgtggt gatgtgaaat atcgttttgt
     7921 tatcgataat cgcacactaa cagattgaaa aaattaataa atacgctg
//
LOCUS       DAJTZL010000171         7944 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.171, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000171 DAJTZL010000000
VERSION     DAJTZL010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7944
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.171"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..359
                     /locus_tag="OO544_003424"
     CDS             <1..359
                     /locus_tag="OO544_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="acts in anaerobic beta-oxidation of fatty acids;
                     possible ligase/synthase involved in anaerobic growth on
                     fatty acids; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cyclohexanecarboxylate-CoA ligase"
                     /protein_id="HCR6768944.1"
                     /translation="GRKKDIIVRGGENISSREVEDILLQHPKIHDACVVAMPDERLGE
                     RSCAYVVLKAPHHSLSLEEVVAFFSRKRVAKYKYPEHIVVIEKLPRTASGKIQKFLLR
                     KDIMRRLTQDVCEEIE"
     gene            complement(416..2794)
                     /gene="ppsA"
                     /locus_tag="OO544_003425"
     CDS             complement(416..2794)
                     /gene="ppsA"
                     /locus_tag="OO544_003425"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HCR6768945.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHEISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVEMMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            3127..3960
                     /gene="ppsR"
                     /locus_tag="OO544_003426"
     CDS             3127..3960
                     /gene="ppsR"
                     /locus_tag="OO544_003426"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HCR6768946.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            4117..5163
                     /gene="aroH"
                     /locus_tag="OO544_003427"
     CDS             4117..5163
                     /gene="aroH"
                     /locus_tag="OO544_003427"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HCR6768947.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGAATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYAPRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLLEGTQKIVGSQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            5295..5486
                     /gene="hemP"
                     /locus_tag="OO544_003428"
     CDS             5295..5486
                     /gene="hemP"
                     /locus_tag="OO544_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HCR6768948.1"
                     /translation="MRYTDSRKLTPETDANHKAASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(5490..6926)
                     /locus_tag="OO544_003429"
     CDS             complement(5490..6926)
                     /locus_tag="OO544_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HCR6768949.1"
                     /translation="MTLSFVTRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNGAGVWGGEALLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TAEPGAMLMRVAPSHLRFGHFEHFYYRRESEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FSDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FITEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSTASPLRDEFIDRAAFDGW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMTELHRLHGAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(6989..7702)
                     /gene="ydiV"
                     /locus_tag="OO544_003430"
     CDS             complement(6989..7702)
                     /gene="ydiV"
                     /locus_tag="OO544_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HCR6768950.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQVLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLERDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRGLLSLSQVYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTAEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
BASE COUNT         2030 a         2085 c         1888 g         1941 t
ORIGIN      
        1 ccggacgcaa aaaagatatt attgtccgcg gcggcgaaaa tattagcagc cgtgaagtag
       61 aagatatttt attgcagcat cctaaaattc acgatgcctg tgtggttgca atgcccgatg
      121 aacgtttagg tgaacgatca tgcgcttatg tcgtgctgaa agcgccgcat cattcattat
      181 cgctggaaga ggtggtggct ttttttagcc gtaaacgggt cgcaaaatat aaatatcctg
      241 aacatatcgt ggtaatcgaa aaactaccgc gtactgcctc aggtaaaata caaaagtttt
      301 tgttaagaaa agatattatg cggcggttaa ctcaggatgt ctgtgaagag attgaataag
      361 tttcatcttc ggggatcaca taaccccggc gactgaacgc cgccggggat ttattttatt
      421 tcttcagttc agccaagctt aaccaggttt gcaccacggt gtccgggttc agagacaggc
      481 tatcgatccc ctcttccatc aaccatgcgg caaagtcttc gtggtcggac ggaccctgac
      541 cgcaaatccc gacatatttg ccctgtttct tcgcggcacg gatagccatc gacagcagtg
      601 ctttcaccgc atcgttgcgc tcatcgaaca attcagacac cacgccggag tcacggtcca
      661 gaccgagcgc cagctgcgtc atatcgtttg agccaattga gaagccgtcg aaatattcga
      721 ggaactgctc ggccagcaag gcgttggacg ggatttcaca catcatgatg attttcagcc
      781 cgttctcacc acgcttcagc ccctgacgcg ccagttcctc aaccaccgct ttcgcctgat
      841 ctacggtacg cacgaacggg atcatgatct caacgttggt cagccccatg tcgttgcgca
      901 cacgtttcac tgcttcgcac tccagcgcga agcagtcgcg gaagctgtcg gaaacatagc
      961 gtcccgcgcc acggaagccg agcatcgggt tctcttcatc tggctcgtaa cgctcaccac
     1021 cgaccaggtt ggcatattcg ttcgatttaa aatcagagag acggacaatg acgcgcttcg
     1081 gataaaacgc ggcacccagc gtcgcgatcc cttcagtcag acgaccaacg taaaattcac
     1141 gcggagaatc aaaacctttc atcatctcgc ggatttcatt ttgcaactgc ggttcctgat
     1201 cgtcaaactc aagcagtgcg cgtgggtgga cgccaatcat acggttgatg ataaattcca
     1261 gacgcgcaag gcccacgcct tcgttcggca ggcaggcgaa gtcgaaagca cggtccgggt
     1321 taccgacgtt catcatcact ttcaacggca gatccggcat catttctacg ctggagcttt
     1381 tcacgctaaa ttccagcaac tccgcataga cgtaaccggt atcaccttcg gcacaagaaa
     1441 cagtgacgtt ctcaccgtct ttcatccgtt ctgttgcatc accacagccc actaccgccg
     1501 gaatgcccag ttcacgagcg atgatcgccg cgtgacaggt acgaccgcca cggttggtga
     1561 cgatggcaga tgctttcttc atgatcggtt cccagtccgg gtcggtcatg tcagtgacca
     1621 gcacgtcacc aggttcgatg cggttcattt cgctgatctc atggatgact ttcaccggac
     1681 ccgcgccgat gcgatgaccg atagcacggc cttcggcgat aatcttaccc tgtgaatgca
     1741 gcgtataacg ctccatgacc tgaccgcgtg agcgcacggt ttccggacgc gcctgcacaa
     1801 tgaacagttt gccagtgtgg ccatctttcg cccactcaat atccatcggg cgaccgtagt
     1861 gtttctcaat ttgtacggcc tgttttgcca gttcctgcac ttcttcgttg gtcagcgaga
     1921 agatgtcacg ctgttcctgc ggtacgtctt cgattttaac ctgcttgccg tgctcctggg
     1981 tcggcgcgta aaccatgcgg atttttttcg accccatggt gcgacgcacg atagccgggc
     2041 gattcgccgc cagtgtcggt ttatggacgt aaaactcatc cgggttaacc gcaccctgca
     2101 cgaccatttc cccaaggccc catgcggaag tgataaacac cacctggtca aagccggatt
     2161 cggtatcaat ggagaacatc acgccagaag atgcgaggtc ggagcgcacc atccgctgaa
     2221 caccggcaga gagcgccacg ccacggtgat cgtaaccctg gtgcacacga taagagatgg
     2281 cgcgatcgtt aaacagagaa gcaaatacat gcttcactgc cacgagaacg gcgtcaaaac
     2341 cctgaacgtt gaggaaggtt tcctgctgac cggcaaaaga agcgtccggc atatcttctg
     2401 cggtggcgga ggagcgcacc gcaaaagagg cgttttcgtc atcggcggaa agctgtgcat
     2461 aggcttcgcg gatggcgttt tccagctcag gctggaaggg agtgtcgata atccactggc
     2521 ggatttgcgc gcccgctttc gcaagctgag taacatcgtc aatatccgtt ttatccagca
     2581 gttcataaat gcgctggttt acgccgcttt ggtccagaaa ctggttaaac gcgtcggcgg
     2641 ttgtggcgaa accattcgga acggaaacac ccattccgga aaggttagta atcatttcac
     2701 ccagggaggc atttttgccc ccaaccctgt ctacatcatt catgccgagt tggttatacc
     2761 aaagcaccag cggtgacgag ccattgttgg acatcgaaca atccttttgt gataaatgaa
     2821 cggtttgaga aacacatttc tgcgcattta tctttgcata tttaaccgga tgaaaaaaac
     2881 ggtgaatcgt tcaagcaaat atattttttt actttttaag actgatccca gcgttgcgca
     2941 aatctgctct cttcgacaat ttccacataa accatcggta taaacgaaac cataaaaatg
     3001 aaatgctgtt ttcataaaaa ataaaattga aggttcattt tataaaccag acataacgtc
     3061 ctacgcttct gtgcgttttt aatttatgct ttcatagaat tatgtctgca tcacgggaag
     3121 aacaaaatgg ataatgctgt tgatcgccac gttttttata tttctgatgg tacggcaata
     3181 actgcggagg tattaggaca cgcagtaatg tcacaatttc ccgtcactat cagcagcatt
     3241 acgctgccgt ttgtcgaaaa tgagagccgt gcacgggcag tgaaggatca gattgacgca
     3301 atttatcacc agacaggtgt gcgcccgctg gtcttctact ccatcgtgtt gccggagatt
     3361 cgcgccatca tcttgcaaag tgaaggcttt tgccaggata tcgttcaggc gctggttgcc
     3421 ccgctacaac aagagatgaa actggatcca acgccgattg ctcatcgtac ccatggcctt
     3481 aaccctaata atctcaataa atatgatgcg cgcattgcgg cgattgatta caccctcgcc
     3541 cacgatgacg gcatttcgtt gcgcaatctg gatcaggctc aggtgatcct gctcggtgtt
     3601 tctcgctgtg gtaaaacccc caccagtctt tatctggcaa tgcaatttgg tatccgcgcg
     3661 gcaaactacc cctttattgc cgacgatatg gataatctgg tgctacccgc gtcgctcaaa
     3721 ccgcttcagc ataaattgtt cggcctgact atcgacccgg aacgtctggc ggcgattcgc
     3781 gaggaacgtc gggagaacag tcgctatgcc tcgcttcgtc agtgcaggat ggaagtcgcg
     3841 gaagtggaag cgttgtaccg taaaaatcag atcccgtgga ttaacagtac caattattcg
     3901 gtagaagaga ttgccaccaa gatcctcgat atcatgggcc ttagtcgccg aatgtactag
     3961 agaactagtg cattagctta tttttttgtt atgatgctaa ccacccggcg aggtgtgaca
     4021 cacctcgcac ttgaaatcag cagcgattgg tttatcgtga tgcgcatcac ttcccggcag
     4081 tcctgccgta gaagcaacaa atttctgaga cttgtaatga acagaactga cgaactccgt
     4141 actgcgcgta ttgagagcct ggtaacgccc gccgaactcg cgctacggta tcccgtaacg
     4201 cctggcgccg ccacccatgt caccgactcc cgccgcagaa ttgaaaaaat actcaatggt
     4261 gaagataagc gactgttggt cattattggc ccctgctcga tccacgatct caccgctgca
     4321 atggagtacg ccccccgtct gcagtcgctg cgcaaccagt accagtcacg gctggaaatc
     4381 gtaatgcgca cctattttga aaaaccacga actgttgtcg gctggaaagg actaatctcc
     4441 gatccagatt taaacggcag ctatcgggta aatcacggtc tggagctggc gcgcaaatta
     4501 cttttacagg taaatgagct gggcgtccca accgcgactg agttcctcga tatggtgacc
     4561 ggtcagttta ttgctgattt aatcagttgg ggcgcgattg gcgcacgtac taccgaaagt
     4621 cagatccacc gtgaaatggc ttcggcactc tcctgtccgg taggttttaa aaatggtacc
     4681 gatggcaata cgcggattgc tgtggatgct atccgcgcag cccgcgccag ccatatgttc
     4741 ctctcgccag acaaaaatgg tcagatgacc atctatcaga ccagcggcaa cccgtatggc
     4801 cacattatta tgcgtggcgg caaaaaaccg aattatcatg ccgatgatat cgccgcagcc
     4861 tgcgatacgc tgcacgagtt tgatttacct gaacatctgg tggtggattt cagccacggt
     4921 aactgccaga agcagcaccg tcgccagtta gaagtttgtg aggatatttg tcagcaaatc
     4981 cgcaatggct ctacggcgat tgctggaatt atggcggaaa gtttcctgct cgaaggaacg
     5041 caaaaaatcg tcggcagtca gccgctcact tacggtcaat ccattaccga cccgtgtctg
     5101 ggctgggaag ataccgaacg cctggtcgaa aaactcgcct ctgcggtaga tacccgcttc
     5161 tgaatgcgtg cccattcctg acggaatggg catttctgcg caacttgttg tcttctcaac
     5221 aaattactgc ttgctctggc ctgccataat attgataata agaatcattg ttatatcaat
     5281 tattattaat ctttatgcgt tatacggata gcagaaaact cacgcctgaa acggacgcca
     5341 atcacaaggc cgcttccccg cagcccatcc ggcgaattac cagccagaca ctgttaggtc
     5401 cggatggcaa actgattatc gatcatgacg ggcaagaata tctgctccgt aaaactcagg
     5461 ctggcaagct gctgttaact aagtagcctt taactcgagc agctgacttc cagccgttta
     5521 ccccagtcag gtggacgact gacatagtca tcatctctat cgctgaaagg atttcgcaac
     5581 gccccatgca ggcggtgcaa ttccgtcata tcaccctttt ctgccgcttc aatcgcccgt
     5641 tgcgccaacc agttgcgcaa taccagagcg gggttaacgc tttgcatcaa ctgctgacgc
     5701 tcactatcgc taacctcgtc ttgttgcaaa cgcccccgat aacgggcaaa ccagccatca
     5761 aatgccgcac gatcaataaa ctcatcacgt agcggtgacg ccgtgctgtg ctgctcggtc
     5821 agactcagca tgcggaatgt gcgggtataa tcgctgcgct ctcgcgccat cagactgaat
     5881 aattcattca gtagcgcgtt atcctctttt tgctccgtga tgaagcccag tttctgccgc
     5941 atccgttgtc cataatgcgt caacaaaacc tgctgatagc tgtccagggc ctcattcagg
     6001 gcatctacgg caacaaatgg tgacaatgtc tgcgccagac gctgtaaatt ccacaacgcg
     6061 acagcaggtt gattatcaaa actataacgc ccttgatgat ccgagtgatt acaaataaaa
     6121 ccgggttcgt aatcatcaag aaaaccaaac ggcccgtaat caagcgtcag tcccagcagc
     6181 gacatgttgt cggtattcat caccccatgt gcaaagccga ccgtctgcca ttgggcaatt
     6241 aacgacgcgg tacgtgcgac aacatcgcta aaccagagac ggtatttgtc ctcatcatct
     6301 tcaagatgtg accagtaatg acgaatagca aagtcagcta actgacgaac cttttccgac
     6361 tcgcggcggt agtaaaaatg ttcgaaatga ccaaagcgca gatgacttgg tgccacacgc
     6421 atcagcatcg cgcctggctc cgccgtttcc cgatacactg gcgaatcgct ggtgacgata
     6481 cttaacgcgc gggtcgtcgg aatgcccaga taatgcatcg cctcactggc gagactttct
     6541 cgtatcgtcg aacgtaaaac cgcccgtcca tcacccattc gcgaataagg cgtcaggcca
     6601 gcacctttca gatgccagtc cattgtagtg ccatcagcaa gcagttgttc gccgagtaaa
     6661 atgccgcgcc catcacccag ttggcccgcc cagacgccga actgatgacc actgtaaacc
     6721 tgcgccagtg gtgacatacc aggcagtagg gcttcgccgc cccagacacc tgcgccattt
     6781 ttaaacagcg acgatggaat actcagcgtg ttagccagtt cggtattatg ccaaatcagc
     6841 cgggcattat ttaaaggcgt aggggaaagt gctgtatagg tttctggcaa ttcatcgcgc
     6901 cagcgggtaa caaaagacag ggtcatagat cctcctgttt gatagtgtag acggttactc
     6961 tcgtcttaaa caccagcaaa cagaagggtt atcgctgaac caacgtcgtt atctgattta
     7021 tgggaacggc aggccacagg catccctgga gagcatggaa atcgaagggg gtaatctgtg
     7081 ccaatatttc tgccgtatca atgcctcccg caatgatgca attgcaacaa ggggaaattt
     7141 gcgcctggat cgcacgaata aacggttcga aggagcggtg cgtgatttgt tgctgaataa
     7201 aacttttatc cagcatgacg cgggtgaaaa gaccatcaaa aaccgctttc attgtgctat
     7261 tgcctgctcc caaattacct aacacaaggg gatacacttg cgataaggat aaaagaccgc
     7321 gattatcttt tccttcattg agatgcgggt aattctcatt aatcaacagt tcaataaacg
     7381 gatattttaa tagttcgcct gcgtaattat ccctctctaa taataatgta gccacttgtg
     7441 gcgttaaatt aagccaggca aagagtttat gttgaataaa aaaatgttgg catgatttta
     7501 gtaattccaa ctgctctgaa aataattgcc aatgttgctc ctcggtaagt tgcgctataa
     7561 cccgactcgt cgggatacgc accgtgccat cttcactgga gaaatgggta attaactcaa
     7621 cgccgacaag gacttgctgg ttatctctta tcggcaggaa atagcaatca gaatgataaa
     7681 gattctccaa aaaaatcttc atggtaaccg tccctctcga aggatgtttt tcagtatccg
     7741 gctaaaaggg atgaggccat aagacataac aattacagaa ggagtaactt tcatttgttc
     7801 catgttaacc actttttcag gggtcctttt tttagattat cctgattata aacgaataat
     7861 ccatttacgg aatttttgtc tgcaaaatac tactgtattc aggagtaaat ggacggcgat
     7921 gtatcatcgc cgtccatttt tcga
//
LOCUS       DAJTZL010000172         7944 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.172, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000172 DAJTZL010000000
VERSION     DAJTZL010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7944
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.172"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            111..443
                     /locus_tag="OO544_003431"
     CDS             111..443
                     /locus_tag="OO544_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HCR6768951.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            590..1951
                     /gene="yegQ"
                     /locus_tag="OO544_003432"
     CDS             590..1951
                     /gene="yegQ"
                     /locus_tag="OO544_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HCR6768952.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(2281..2598)
                     /locus_tag="OO544_003433"
     CDS             complement(2281..2598)
                     /locus_tag="OO544_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6768953.1"
                     /translation="MKKIAAISLISIFLISGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            3004..3903
                     /gene="yegS"
                     /locus_tag="OO544_003434"
     CDS             3004..3903
                     /gene="yegS"
                     /locus_tag="OO544_003434"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HCR6768954.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARYVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDGIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCLLLR"
     gene            complement(3985..4758)
                     /locus_tag="OO544_003435"
     CDS             complement(3985..4758)
                     /locus_tag="OO544_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR6768955.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSEVDKLALQKTRVKLITV"
     gene            complement(4864..5904)
                     /gene="gatD"
                     /locus_tag="OO544_003436"
     CDS             complement(4864..5904)
                     /gene="gatD"
                     /locus_tag="OO544_003436"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCR6768956.1"
                     /translation="MKSMVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTYPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFTQAVRDIA
                     RNAMPGKVLLIP"
     gene            complement(5952..7307)
                     /locus_tag="OO544_003437"
     CDS             complement(5952..7307)
                     /locus_tag="OO544_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HCR6768957.1"
                     /translation="MFSEVMRYILDLGPTVMLPIVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSTQNIPGFIIIGAIYLTGIFMTWRRARGFIKQEKTVLA
                     E"
     gene            complement(7311..7595)
                     /gene="gatB"
                     /locus_tag="OO544_003438"
     CDS             complement(7311..7595)
                     /gene="gatB"
                     /locus_tag="OO544_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HCR6768958.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQNHSIPVELIQCRVNEI
                     ETYMDGVHLICTTAKVDRSFGDIPLVHGMPFISGVGIEALQNKILTILQG"
     gene            complement(7626..>7944)
                     /gene="gatA"
                     /locus_tag="OO544_003439"
     CDS             complement(7626..>7944)
                     /gene="gatA"
                     /locus_tag="OO544_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HCR6768959.1"
                     /translation="EFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADD
                     DNDVEVSLVIALIVENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLD
                     SDE"
BASE COUNT         2148 a         2036 c         1783 g         1977 t
ORIGIN      
        1 tgacattatc tctccgccct ggcaggcatc agaaaatcgc tcatacttta atcggtaaac
       61 agcaccttta gatgctgttt tcgatacgca atttcaatca aggagtcatt atggctggtt
      121 ggtttgaact cagtaagagc agtgataatc agttccggtt tgtgctaaaa gcgggcaatg
      181 gtgagactat cctcaccagc gagctgtata catcaaaagc ctctgcggaa aagggcatcg
      241 cgtcggtgcg tagcaacagc ccgcaagaag aacgctatga gaaaaaaacg gcaagtaacg
      301 gcaaattcta tttcaatctg aaagccgcca atcatcaaat tatcggctcc agccagatgt
      361 acgccaccgc gcaatctcgt gaaaccggaa ttgcctccgt taaagccaat ggcacaagcc
      421 agacggtgaa agacaatacg taattacgat gccgggcgtg gggtgtcggc gtccggcgct
      481 ttaccagatc cccccacagc gctacaatgc ccgcccttaa agtgggggca ctcccctaac
      541 cgcttcatca ggtgaagcgg agctgacctg tcatcagaac gagagaatta tgtttaaacc
      601 ggaactcctt tccccggcgg gaacgctgaa aaatatgcgt tacgctttcg cttatggcgc
      661 agatgctgtt tatgcgggcc agccgcgtta ctccctgcgt gtgcgcaaca acgaattcaa
      721 ccacgaaaat cttcagctcg gcatcaatga agcccacgcg ctggggaaaa agttttatgt
      781 cgtggtcaac attgcaccgc acaacgccaa gctgaaaacc tttatccgtg acctgaaacc
      841 ggtggtggaa atggggccgg atgcgctgat tatgtccgat ccagggctga ttatgctggt
      901 gcgtgagcac ttccctgaaa tgccgatcca cctctcggtg caggctaacg ccgtaaactg
      961 ggcgacggtg aaattctggc agcaaatggg cctgacccgc gtgatcctct ctcgcgagct
     1021 gtcgctggaa gagattgaag agatccgcaa tcaggtgccg gatatggaga ttgagatctt
     1081 cgttcacggt gcgctgtgca tggcctactc cggtcgctgc ctgctctctg gctatatcaa
     1141 caagcgcgat ccgaaccagg gtacctgcac caacgcctgc cgctgggagt acaacgtcca
     1201 ggaagggaaa gaagatgacg ttggcaacat cgtacacaag tacgagccga ttccggtgca
     1261 aaatgttgag ccgacgctag gtatcggcgc gccaaccgac aaagtgttta tgatcgaaga
     1321 agcccagcgt ccgggcgagt atatgaccgc gtttgaagat gagcacggca cttacatcat
     1381 gaactcgaaa gatctgcgcg ccatcgccca tgtagaacgc ctgaccaaaa tgggcgtgca
     1441 ttcgctgaaa atcgaaggcc gtactaaatc tttctactat tgcgcacgca ccgcacaggt
     1501 ttatcgtaaa gctatcgatg atgccgctgc gggcaaaccg ttcgatacca gcctgctgga
     1561 aactctggaa ggtctggcgc atcgtggcta taccgaaggt ttcctgcgtc gtcatactca
     1621 cgacgattat cagaactacg aatacggtta ttcagtttct gaccgccagc agtttgttgg
     1681 tgagtttacc ggtgagcgca agggggacct cgcagcggta gcggtgaaaa ataaattctc
     1741 cgttggcgac agccttgagc tgatgacgcc gcaaggcaac attaacttta cccttgagca
     1801 catggaaaac gccaaaggtg aagcgatgcc ggtcgcacca ggcgatggtt atactgtgtg
     1861 gctcccggtc ccgcaggatc ttgagctaaa ttacgcgctg ctgatgcgta atttctccgg
     1921 ggaaaccacg cgtaacccac acggtaagtg attaatttcg attatttttc ccggatggaa
     1981 aattcttaga aaccgatcac atacagctgc atttattaag gttatcatcc gtttcgctga
     2041 aaaacataac ccataaaatg ctagctgtac caggaaccac ctccttagcc tgtgtaatct
     2101 cccttacacg ggcttatttt ttatgcataa gccctatccc tggtcaccgt cttccattga
     2161 ccacatcgat agaatctccc ttcatagcac gatgcctttc acgtaacggc atcgtgctcg
     2221 cacaggttcc ggctaagcac aaccagaacg cgcatgtttg atgcttacca aaaaatattc
     2281 tcactctcca catttgaatg tcagacgagc gacgccatgt aatcctgcac cttctgtctt
     2341 caggtcaact atctgcattt ttttgccctg agtaacacag aaatgggctg catcattttt
     2401 tactatattt tctgcaccag atattcttcc cctagctaaa gaagcttcgg cttcggtgta
     2461 gtattggtta tcgagtttac gctgaatatt acttttatat gcaagaccaa atttaccgat
     2521 acttgtttca tcattatgca cagcacaacc agatataaga aaaatactaa ttaatgatat
     2581 agcagctatc tttttcatct caccttcccc cattaaatac caacgacact ctctagtgtt
     2641 taaatataat aatggcatga ttattataat tgaataggat tataataaat gttctgtaca
     2701 acatttccta cataagtagg aattacggac attgaggccc ttcagggtaa ctccatgggg
     2761 gctttaatat attatattga agatgccact gtttagttga atattaggta tatgctcttt
     2821 tttgaaattt atcggtggca gccgttagta ttcgctgtcc ccattgcaag ctcctggtgg
     2881 taaccactga atcctccata cttgaactga ctttttatcc tccgactttc atcctgttct
     2941 gactccacct tttgttttct gctctacact atctacagac caatcataaa ggcacatacg
     3001 atcatggcag aatttcccgc cagcttactg attcttaatg gcaaaagtac tgacaatcta
     3061 cccttgcgcg aagcaattat gctgttgcgt gaggaaggaa tgacgatcca tgtgcgggtc
     3121 acctgggaga aaggcgatgc cgcacgatat gtagaggagg cccggaagtt gggcgtcgca
     3181 acggtgattg ccggtggtgg cgatggcacc attaatgaag tttctacggc gttgattcag
     3241 tgtgaggggg atggcatacc cgcgctggga attttgccat taggaaccgc caatgatttt
     3301 gccaccagtg tagggattcc tgaggcactg gataaggcgc tgaaactggc aattgccggt
     3361 aacgccattg cgatagatat ggcgcaggtc aacaaacaaa cctgttttat taatatggcg
     3421 acaggcggat ttgggacgcg tattaccaca gaaacgccgg aaaaattaaa agccgcgctg
     3481 ggtggcgtct cttacatcat tcatggctta atgcgcatgg atactctgca accggaccgt
     3541 tgtgaaatcc gcggtgaaaa ctttcactgg caaggtgacg ccctggtcat tggtattggt
     3601 aacgggcgtc aggccggtgg cggtcagcaa ttgtgtccga acgcgttaat taacgatggc
     3661 ttgctgcaac tgcgcatttt taccggcgat gaaattcttc cggctctcgt atcaacctta
     3721 aaatctgacg aagataaccc gaatattatc gaaggcgctt cgtcgtggtt tgatatacaa
     3781 gccccacacg aaatcacttt taatcttgat ggcgaaccgt tgagcggaca aaactttcat
     3841 attgaaatac ttccggcggc gttgcgttgt cgattaccac cagattgtct attattgcgt
     3901 taatcaaact attctctctt gaaccaggga gaatccgaaa tgccggatgc ggtgtaaata
     3961 ccttatccgg catacagaac aatactacac cgtaattaat tttacccgtg tcttctgcaa
     4021 tgccagttta tccacctctg atacagcatc atcggtaata ataatgtcaa ttttttcgat
     4081 tggcagtacc tgattaaaac cgcgacggtt aaatttcgat gaatcgagaa cggcaatcac
     4141 tttattagcg gcggtgacca tcgcaccgct aatcgaataa ccttcattaa aggtcgtaat
     4201 accattaacc gcatcaatac catcagcacc gacaaacatt aaatcggcat taatatcctg
     4261 caatgaacgc tcggcaatgg aaccatgcat cgagcgcgtt ttatggcgga ccgtgccacc
     4321 acagacgacc agagtaatgt ctttattttc ggaaagggca aacgctgccg ggagactgtt
     4381 ggtaatcacc gtgatatttt tagcggtcat taatccttcg gcgataagca ttgtcgtact
     4441 tccgctgtcg agaataacgg tcatcccttc atggatcatt ttgactgccg cctgcgctat
     4501 tctgtttttt ggcgcgctgg cgagctgaaa tcgctcttta aacccgactt cctgggtctc
     4561 ggtttcacta ttaccggaca ttattttcgc cgcaccgcca tgaaagcgcg taaccacgcc
     4621 tttttgttcg agaaagcgca aatcggcacg gattgtcgct tccgaggcag caaatactcc
     4681 cgccagatcc tgaacaagca cggttccctg ttcattcact aattggatga tcttattcct
     4741 tcgctcgaat gaattcatcg tcatttaccc atattcaatt gtggctagtg taaacgaagg
     4801 gggaccattg gtgaataatc tgttttgcac aataccgggc cagcatcacg ctggcccgcg
     4861 gtttcatgga atgagcaaca ctttgcccgg catggcatta cgagcgatgt cacgcaccgc
     4921 ctgggtgaag ctttcaaagc ttccacggtg agcgattaat ggctccaggc ttaacttacg
     4981 ttctgtcagc aaccggctcg ccgtttccca ctcctgcccc ggccaagggc tggaatagtt
     5041 catccaactg ccgataaccg tcagctcttt acgcaatatt ttgccaaacg ttgccgatgt
     5101 taaatgcaga tcctgatgca acgtgcccac cagcgccagt tgggcatgag gaccggcaat
     5161 ctctaccgcc agttcgacag tttgcggtac gccagccgtc tcgaggataa gctgattaaa
     5221 gcgcagttcg cgtaaaacgc tctgcatttg cggcgcgctc atttcgctac tgttaaatgt
     5281 ttgcatcgca ccgaaagatt ttgccaatgc cagtttttct gagctaatgt cgatcgccgt
     5341 cacactcttt gctcccagcg cgacagcgca ctgaatagcc agcaggccaa tggttccggc
     5401 accaataata ataacgtttt tattctcaca accttgcgct aaatgaaaag catgcaggcc
     5461 aacggtaatc ggctcaataa aagccccatc ctcaataggc atatccgtgg gtagagcaaa
     5521 gacatttttt cgcttaacga caatatattc agcaaatcca ccatcacgcc gcgagccaat
     5581 aaaatcatat tttgcgcact gggaataaaa ccctttcaaa cactctggat aagtaaaaca
     5641 gggtaataac ggcacacagg caaccgcatc gccagggtgt aaatcatcaa caccggatcc
     5701 caccgcatca atatagccgc taaattcatg gcctaacgtt attggataat aatgtgcacc
     5761 atttttaaat atcctgggta aatcggaacc acataagccc gagctggcaa tttttacccg
     5821 cacctcatcc tgatgtttaa tttcaggaat gacgctttct gcaacgcgca cgataccatc
     5881 agtatcattc accattgatt tcataaaaac tcctgattgt taagggggat aacctccccc
     5941 tcaggtaaaa attattctgc gagaacggtt ttctcttgtt taataaaacc acgcgctcta
     6001 cgccaggtca tgaaaatacc ggtaagataa attgcgccga taataatgaa accgggaata
     6061 ttttgcgtgg agaaaacctg aatcagtaac caggtaatgg gagaaccgcc ctgatccatt
     6121 gaagccacca tacccccggc ttttaacgcc ccagcattag ccgccagttg ggtgtgcaaa
     6181 ccaatagttt gcgtcgcgat ccacagggtg atgctcataa tgatgacacc cgagattaag
     6241 gtgcggaaca gatttccacg atgcaccgcg accgccatcg ccacgaagaa gccgatggtg
     6301 gcaagatcgc caaacggcag tacctgatta cccggaacac aaacagcaat taaaatggtg
     6361 agcgggataa aaatcaggct tgccgatacc accgccgtat gccccagcag taatgcggga
     6421 tccaggccaa tcaggaactc ctgaccgccg aacttcgcct gtaaacgact acgggcctgc
     6481 ttagcgatgg gcgttaaacc atccatgatg ggtttaatca cccgtggcat cagcagcata
     6541 actgccgccg tttttaccgc cagctgcaat acacctttga catcgtaacc ggcgaggatg
     6601 ccgataatca gccccatcac aaaacccacg gtgacaggct cgccaaatgg accaaatttg
     6661 cgctgaatat cgtcggcgct aaatttaatt cggttaacgc ctgggatttt ctcgatgata
     6721 gcatcgacca gcaccgcaat cggccccata tacgccgacg taccgtgcgg aatagcaata
     6781 ccttccagct caaagaaatt tcgggtatcg cgggcaaacc agtcgccgag cttataaaca
     6841 aacgccgcgt gaattaccac acctgccatc cctatcatcc atgaaccggt tgccagatgc
     6901 agcaacgcgc cggtgaaggt catatgccag atattccaga tatcaacatt taccacccgc
     6961 gtcatacggg tcagtaacat cgccacgtta accagaatcg caatcggaat cgccaccagc
     7021 gcaatttgcg acgcccaggt cattggtgaa gagcccggcc agccgacatc gaccacatgc
     7081 agattcaggt cgaaattttc cgccatcgct ttcgccgccg gaccaatgga atccagcatt
     7141 aagccaatca caaggccaat gccaacaaag ccaatcccga tatgcagacc cgctttaaag
     7201 caatcgcctg ccttcatgcc taatatttta gaaaaaataa tgatgacaat cggaagcatc
     7261 accgtagggc cgaggtcgag aatataacgc atgacttctg aaaacatagg tcacccctgt
     7321 aagatagtca gaattttatt ttgtaatgct tcgataccga caccagaaat aaaaggcatg
     7381 ccgtgaacta acggaatatc gccaaaacta cgatccactt tggcagtggt gcatatcaaa
     7441 tgcacaccat ccatataggt ttctatttca ttaacccgac actggattaa ttcaacagga
     7501 atactatgat tctgacacaa ctctttaatt tcttccgccg ccatcgtaga ggtcgcaacc
     7561 gcgcctccgc aagcgacaat aatcttgcgt ttcataaata gtccttttta ttgttacaga
     7621 gggatttatt cgtctgaatc taaaacatac tttgtgaagt attcctttaa ctgggtttca
     7681 ggaagagtga ttagtgtctc gacgatatcg ggctgttgta acttgccaaa taaacagcgt
     7741 aaaagtttca attgctgctg cggattctcc acaatcaacg caataaccaa cgatacctcc
     7801 acgtcgttat catcatccgc ttgctgaaaa tgaactttat ttgttggcct taacagataa
     7861 atggctgacg acttagcatg aatcgcctca caatgcggta ttgcaatggc gtgctgctca
     7921 agcattatcc cggtagggaa ttct
//
LOCUS       DAJTZL010000173         7879 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.173, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000173 DAJTZL010000000
VERSION     DAJTZL010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7879
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.173"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..200
                     /locus_tag="OO544_003440"
     CDS             <1..200
                     /locus_tag="OO544_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6768960.1"
                     /translation="VCCGDTLLPLTIINRGGLRGRPTIYPPETLDSFIKLYRQGKPIK
                     CIADALRMPLSTSYRIAQRLS"
     gene            583..1536
                     /locus_tag="OO544_003441"
     CDS             583..1536
                     /locus_tag="OO544_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HCR6768961.1"
                     /translation="MEKFHMFDDRIYQMTNGVKAADRIIYTVMLNEQKFYHDKGQYYT
                     PSYAHLGQYAGITRTSVIACMKQLEAHGLIKKTNVASNQCVIKVYSLDEVPEVLTELT
                     AKEARKAKLESKVTQSVKKDDPLSQNSLPTESKFFTQSVKIFDTYNNSNNKKELINNN
                     KEDDFEEEYFKKGASLCDSHECDSLGIEEAIKETVKTSDLDDLDSEAAKEVVKKEDKI
                     SDVIASSDHKNTQCPATDDELPDVDLDDEEDPEKELKREQRKAEIKERFRNAKLKNKV
                     SDLDDFDDSDDDFTFTPKKKTHTEPKKEKVKVRRVYGGGMF"
     gene            1536..1832
                     /locus_tag="OO544_003442"
     CDS             1536..1832
                     /locus_tag="OO544_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000860811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6768962.1"
                     /translation="MKTYNGERKYRDYLLRRYSISREGYLMKDTHGEVYRIRPKKEGR
                     DYYFVDDVTDLKIDALKFAVLYHYDLWDSVHQLRLKDGDPGNLRATNIIKGKCR"
     gene            2156..2446
                     /locus_tag="OO544_003443"
     CDS             2156..2446
                     /locus_tag="OO544_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000149937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6768963.1"
                     /translation="MTHTVTRLLPEAFERLAAIKAKYPDMKHTALVGEILKESVNLSP
                     FDNVKGVAEGSKAIRITTGQLELLKAQSAVAGCSMIQVLSAKVHQLADRLEV"
     gene            2667..2882
                     /locus_tag="OO544_003444"
     CDS             2667..2882
                     /locus_tag="OO544_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6768964.1"
                     /translation="MKTITVQLQTNKAFRYFENLLELYEGWGSIHGKDDIYLHLSAPN
                     YSLKTPVKQSWLKDYGHQMGLLVSDLS"
     gene            3029..3334
                     /locus_tag="OO544_003445"
     CDS             3029..3334
                     /locus_tag="OO544_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6768965.1"
                     /translation="MNYPMQIMVDQETMNWLIDLNRSGFNIYFMHCFEASTYIDKHII
                     NEIKLGDYVHKLEVTDPYNKRFIIEECNRLDLSPEQLLKLNVFLTYQNELNPYSEVM"
     gene            3810..4520
                     /locus_tag="OO544_003446"
     CDS             3810..4520
                     /locus_tag="OO544_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6768966.1"
                     /translation="MTNDIYINGKVIDSFSDWSPQTEHPAITIQRKEHDKRIELEQSL
                     KMSPKTTIFVSPEPQEMPDICKSEALLELEKKYYQLLKEQRLRLDDAYSKVTLMQSAI
                     EPSEFEIQDELSQKPFVYYQYEDNDGFGTFQENVAAVISSLPAGHRIVKMVKASRGAG
                     QFIYMTNKSDEELAEIARQNILASRNKQLDNVKLYLSRELQAMKELVKEYEDAKKIAM
                     QADIEQLTKISSKYAKAI"
     gene            4545..4751
                     /locus_tag="OO544_003447"
     CDS             4545..4751
                     /locus_tag="OO544_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6768967.1"
                     /translation="MIDLTERYELIKAISERLKEQPNKPKRIKSFTSSKQKNRIRKIA
                     KWCLDKLPSDCQIVGEDGNYYLVR"
     gene            4850..7030
                     /locus_tag="OO544_003448"
     CDS             4850..7030
                     /locus_tag="OO544_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HCR6768968.1"
                     /translation="MSKFTVDSFIVELSFSENVIKGLQRVEKAALQSAQRIERNLNRA
                     FKVDTKQLDSNLTSSLGQLERKFNKTFDKIEQRARSTKAFQFATRFNDTVNPPKQSRQ
                     PRISGNRAITAAHSVNMSKLGDINPLMAKMFKAQYYSLSGKAGNIGSEKFNAELAKLN
                     QSLRETLNKLRSQTKTASINNTSDAFNNLASNAIKLSGAFYSVMGALNAYKAIMNAGL
                     KRDSAQRAAKFVLGDKASEAEVFIRNLADKTGLNISEGLSSYAKFAAGAQGSMSQEQT
                     QELFGNATAMSRLMGLSNDELNGILRAFEQMASKGKIQAEELRGQLGDRMAGAFKLFA
                     EALGMTATELDAAMKNGKVLSADTLPKVAKQMGLMIDKAGGWAEVTKSTQTALGKLAN
                     NWDDTMVKIFSGSQDELNGFLSSLSNLLSEMGMSSSIAGDAIGGLIDMLKAGVDDIRV
                     FNNTIEGWILQTKKFYYSLDDTKRKLLDEVGDGFINFVKGLAIALSAKTLFSATTGVM
                     NLTRAITTLGTRANQVAGQVATGKGGGKLKGVAGGVGSALAIGYADDGYETALALASM
                     IPQIRGVTLGLYALKKALDFMNQEVVKNANMHPSGVGVGSDFNPVYSGDPNKPNMINE
                     GWSRIFSSMGELFNNATMNIARFNHPELNNIKQDSALTSADIQGLRDEISALSKRIQE
                     PVKVSLGGQVVVRPDEPSFFTFNQSANDQFTQDMLLSSSYPEEE"
     gene            7187..7775
                     /locus_tag="OO544_003449"
                     /pseudo
     CDS             7187..7775
                     /locus_tag="OO544_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000640586.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2599 a         1402 c         1688 g         2190 t
ORIGIN      
        1 cggtttgctg tggtgacacg ctattaccgt taaccatcat taacagagga ggcttgagag
       61 gaagacccac catttaccca cctgagacgc tcgacagctt cataaaacta tacaggcaag
      121 gaaaacccat taagtgcatt gcagatgctc tcagaatgcc tctgagcacg tcatacagga
      181 ttgctcaaag gttgagctaa aattctcata aatatggtca tttacaagat aggtgattca
      241 gatattaaga agtatccctg tgaacaataa atgaccaatc agtaattatt ctgtgaaata
      301 attgttgcaa tttataatca catctgttat agtttttata tggggtgata aaagaactca
      361 gaacattaag aggttacaac gttgggaggc gtcagggtat tgacagattt tcacggcctc
      421 ttattcaatc aaggcttttc cttttgctgt taatactcgc gtaatcgctc aggcttctga
      481 gttcctttat cagtccatct tctaattaat tcactccctc tggggttggt cactatacgt
      541 ggtcagccct ttctttgttt tatttttaaa aaggaaaata taatggaaaa attccacatg
      601 tttgatgacc gtatttatca gatgactaat ggcgttaaag ctgccgaccg tattatttac
      661 actgtcatgt tgaatgaaca aaagttttat cacgataagg ggcaatatta taccccttca
      721 tatgctcatc ttggtcagta tgctggaatc acaagaacat ccgtgattgc atgtatgaaa
      781 caacttgagg cacatggttt aatcaagaaa actaacgttg caagcaatca gtgtgttatt
      841 aaagtttatt cacttgatga agtaccggag gttttaacag agcttacagc taaggaagcc
      901 agaaaggcaa agctggagtc taaagttact cagtcagtca agaaagatga cccactgagt
      961 caaaattctt tacccactga gtcaaaattt tttactcagt cagtcaaaat ctttgacacg
     1021 tataataaca gtaataacaa gaaagaactt attaataaca ataaagaaga tgactttgaa
     1081 gaagaatatt ttaagaaggg agcttcgctc tgtgattcac atgaatgtga ttctcttggt
     1141 attgaagagg ctattaaaga gactgttaaa acatctgact tagatgacct tgatagtgaa
     1201 gctgctaaag aggtcgttaa aaaggaagat aaaatctctg atgtgattgc tagtagtgac
     1261 cataaaaaca cacagtgccc cgccactgat gatgaattac cggatgttga ccttgatgat
     1321 gaagaagacc ctgaaaagga attgaaacgt gaacagcgta aagcagaaat taaagaaaga
     1381 ttcagaaatg cgaagctaaa gaacaaggtt agtgacctcg atgactttga tgatagtgac
     1441 gatgatttta cttttacacc aaaaaagaaa actcacaccg agccaaagaa agaaaaagtt
     1501 aaagtcagac gtgtttatgg tggaggaatg ttttaatgaa aacttataat ggcgagagaa
     1561 aataccgtga ctatttactc agacgttaca gcatttctcg tgaaggttac ttgatgaaag
     1621 atactcacgg ggaagtttac cggattcgac cgaagaaaga agggagggat tattatttcg
     1681 tcgatgatgt gacagattta aagattgacg ctttgaagtt tgcggtgctg tatcattacg
     1741 atttatggga ttcagtccac cagctacgat taaaagacgg tgaccccggt aatctgaggg
     1801 ccaccaatat tatcaaggga aagtgccggt aaggccacca gctatttatt tactgaaatc
     1861 tctatctcaa cacgaagaag tttaagtcga attgtaagct ttgtcttttt tgtaatgact
     1921 cgattcataa cgcaaaacac caatagcaca ataaataccg caaagcccaa cgagctaaaa
     1981 cgatgagatt caataattgc tgtgaaaaaa tcatgcatta attttccttt tgtgaaagga
     2041 aacagcacct gtatcagccc ctacaaccat agtatcatgt aaatgaggta ctgacaatat
     2101 gttaaataga tgttaagatt gtagtatgag taagagattt aaggagattt ttacaatgac
     2161 tcacacagtt acgcgccttt tgccagaggc atttgaacga ctggcagcaa tcaaagccaa
     2221 atatcccgat atgaagcaca cagcgcttgt tggggagatt ttaaaagagt cggtaaacct
     2281 tagccctttc gataacgtta agggcgtcgc agagggttct aaggccatca gaatcacaac
     2341 gggccaactg gaactactca aagcacagtc cgcagtagca ggttgctcaa tgattcaggt
     2401 gttaagcgct aaggtccatc aactggcgga tagattggag gtttaatgtg ttataggttc
     2461 caatattcca cacttggccg ccacctaatc agacagaatt tagcagatgc tgaatttgaa
     2521 gatgtgaccc tcaaagcaaa caaacgatta aaaccctctc aaatccacca gcttaaacac
     2581 gaattaaagc cactggtaat actcgactat aacgaccctg aacagcttga gcagggaatt
     2641 aagcttattt ctgaatgggg tgcttgatga aaactatcac agtacaactt cagactaata
     2701 aagcatttcg ttattttgaa aacttgcttg agttatacga aggatgggga agtatccacg
     2761 gcaaagatga tatctatctt cacttgtcag cccctaacta ttctctgaag actccggtta
     2821 agcaaagctg gttaaaagac tacggtcacc agatggggct attagtctca gacttgagct
     2881 aaaaacagca aaacatatct ctcaggcttt accagacgca ccagaaacgt ttttaagccc
     2941 ctaatcaggg taacttaggg aatcgcctta agtggtctaa aaaaggggtt aaaacgtctt
     3001 agaatgcgat acaggaagga ttttaaaaat gaattaccca atgcaaatta tggttgacca
     3061 agaaaccatg aattggttga ttgacctgaa caggtcgggc tttaatatct atttcatgca
     3121 ttgttttgaa gcgtcaacgt atattgacaa gcatataatc aacgaaatta aacttggtga
     3181 ctatgttcac aagctggaag taacagaccc atacaataag cgctttatca ttgaagagtg
     3241 caatcgactt gatttaagcc ctgagcaatt attgaagctg aatgtattct tgacctatca
     3301 gaatgaatta aacccttaca gcgaggtgat gtaatgacac tcgttattct ctggatgact
     3361 ttagggttat ttgttggttg ctgggtaggt aatacggtaa ataagaattg atgttaccaa
     3421 tctgttaaaa agctgttgac aaataacctt aagcatgaga tgatggtaat agggaagagc
     3481 aactcttcct gaattaaact attaatcatt ctacatcaat gatagttatt ctgaaaggtg
     3541 caatgctttt cgctacatga cctttcaggt tagtctgaga ggtcatctcg aaaagtactc
     3601 ttgttcattc gtttgaatcc tttttgaatc ctttagatgt tttaatttct tcaccttatg
     3661 aatggccctt tataagaaat tatctagggc tattcagctt ttaaaaagca tacttggcaa
     3721 accgcctttt aagtctcttc taagtttaga taggctgtca ggtgtgcttt ttaaaaactg
     3781 aaacacttcc gcttagaaga ggaaaagcaa tgacaaatga tatttacatt aatggcaaag
     3841 tgattgatag cttcagtgac tggtcgccac agactgaaca tccagctatc actattcagc
     3901 gtaaagagca tgacaagcgt attgagcttg agcaatcgct aaagatgtca ccaaaaacaa
     3961 ccatctttgt gtctcctgag cctcaagaga tgcctgatat ttgcaagagt gaagcattac
     4021 ttgagcttga aaagaaatat taccaactgc tgaaagaaca gcgccttaga ttagatgacg
     4081 cttacagcaa agttaccttg atgcaatcag ctattgagcc gtcagaattt gagattcagg
     4141 atgaattgtc acagaaacca tttgtctatt accaatacga agataatgat ggttttggta
     4201 cttttcaaga aaacgtcgca gccgtgatta gtagtcttcc tgctggacat cgaattgtga
     4261 agatggttaa ggcatcaaga ggtgctggtc agtttattta catgactaat aagagcgatg
     4321 aagaactcgc tgaaattgct agacagaaca tcttggcatc acgtaacaag caacttgata
     4381 atgttaagtt gtatctctca agagagcttc aggctatgaa agagcttgtg aaagaatatg
     4441 aagatgctaa gaagattgct atgcaggctg atattgagca gcttacgaag atttctagca
     4501 aatatgcaaa ggctatttaa ttaataatta aaaggtggtg gtgaatgatt gatttaacag
     4561 aacgctatga gcttattaaa gcaattagtg aacgattgaa agaacaaccc aacaaaccaa
     4621 aacgaatcaa atcattcacc tcctctaagc aaaagaatag aatcaggaaa atagctaagt
     4681 ggtgtcttga taaattacca agtgattgtc agattgttgg tgaagatggt aattattatc
     4741 tggttaggta acaagattgg ttgggtgaaa atctacggga gagattatcg agtgatatcg
     4801 gtagtctttc cctttttcgt atatgaacac ttaaagaggt gaaacatgaa tgagcaaatt
     4861 cacagttgat agttttatcg ttgaacttag tttcagtgaa aacgttatta agggcttgca
     4921 aagagttgaa aaggcagctt tgcagtcagc acaaagaatt gaacgcaatt tgaatagagc
     4981 gtttaaggtc gataccaagc aacttgacag caatctgaca agttctttag ggcagcttga
     5041 aaggaagttt aataagacct ttgacaagat tgagcagaga gcaagaagca caaaagcatt
     5101 tcagtttgcc accagattta atgacactgt taatcctcca aaacaatcaa ggcaaccacg
     5161 aattagcgga aacagagcaa tcacagcagc gcatagcgtg aatatgtcta agcttggtga
     5221 tattaatccg ctgatggcta aaatgtttaa agcacagtat tacagcttaa gcggtaaggc
     5281 tggtaatatt ggaagtgaaa agtttaacgc tgaacttgca aaattaaatc aatctcttcg
     5341 tgaaacgttg aataaactca ggtcacagac aaagaccgcc agcattaaca atacaagcga
     5401 cgcatttaat aatcttgcaa gcaatgcgat taaattatct ggtgcatttt actctgtgat
     5461 gggtgctctt aacgcttaca aggccattat gaatgcaggt cttaaacgag attccgcaca
     5521 gagagccgct aagtttgtac ttggtgataa agcatctgaa gctgaggtat ttattcgaaa
     5581 tttagcagac aagacaggtc ttaatatctc tgaagggcta tcaagctacg ctaagtttgc
     5641 agcaggtgct cagggttcta tgtcacagga acagacacag gagctatttg gtaatgcaac
     5701 ggctatgagt cgcttaatgg ggctttctaa cgatgagcta aacggcattt tgagagcatt
     5761 tgagcagatg gcgagcaagg gcaagattca ggctgaggaa ttacgtgggc agttaggtga
     5821 ccgtatggct ggtgcattta agctattcgc tgaagcattg ggtatgactg ctacagagct
     5881 tgatgcagcc atgaaaaacg ggaaggtact ttccgctgat actctcccta aagtcgctaa
     5941 acagatgggc ttgatgattg ataaagcggg tggctgggct gaagtcacta agagcactca
     6001 gacagcttta ggtaagttag caaacaattg ggatgatacg atggtgaaaa tcttctcagg
     6061 ttcacaggat gagctaaacg gcttcttatc cagcctgagt aacctgctta gtgaaatggg
     6121 gatgagttct tctatcgctg gtgatgctat cggtggactt atcgacatgc ttaaagctgg
     6181 tgtagatgat attcgcgtat tcaataacac cattgaaggc tggattttac agactaagaa
     6241 gttctattac agccttgatg acactaaacg aaaactactt gatgaagtcg gtgatggttt
     6301 tatcaacttc gtcaaaggtc ttgctatagc gttatctgca aagacgctat tcagtgctac
     6361 tacaggggtt atgaacctga caagagcaat cacaacgtta ggtacaagag ctaatcaggt
     6421 ggctggtcag gtagctactg gtaaaggtgg tggaaaactg aaaggtgtag ctggtggtgt
     6481 aggtagtgct cttgcaattg gttatgctga tgacggttat gaaacagcgt tagcattagc
     6541 ttcaatgatt ccacaaatca gaggcgtcac tcttggttta tatgcattga agaaagcact
     6601 ggattttatg aatcaggaag tagtcaaaaa cgctaatatg catccgtctg gggtaggtgt
     6661 gggtagtgac tttaatcccg tgtattctgg tgaccctaat aaaccgaata tgattaacga
     6721 aggatggagt agaattttta gttcaatggg tgaattgttt aacaatgcca caatgaatat
     6781 tgcaagattc aatcatccag agttaaacaa cattaagcaa gattcagcat taacgagtgc
     6841 agatattcag ggtctgaggg atgaaattag tgctttgagt aagcgtattc aggagccagt
     6901 gaaagtttca cttggtggtc aagttgtcgt tcgaccagat gaaccatctt tctttacttt
     6961 caatcaatca gctaatgacc agttcacgca ggatatgctc ttaagttcaa gctatccaga
     7021 ggaagaatga tttaacggtg ttctacgtga ataaagcaaa gttaataccc acaccaaaaa
     7081 ttatttttca cgtagctcac gcttaacaga gaaaatacat atatatcaaa ggcttggtta
     7141 aaaatgatta aaatacacta agcctttgaa atcaaaggag aatttaatga tttacactaa
     7201 tcagcttcgt tacagtggca aagatggcat ctatttccac ttaagagaca atgtagatgc
     7261 cttcttaacc ttatcagcaa ctgaaaacat ggaatttgat agccctatgc aggtgactac
     7321 acagaacatg caattatgaa ggaagcttat tattaactcg tcagggtcaa ttagtagaaa
     7381 acttcattga cactctggaa aactggcgtg accagaagca gattatttct gttatctgta
     7441 aagatggaat taaaattgac gattccatta ttaccaactt taaagcctct aaagatgtta
     7501 ctatctctaa cggtcttcgt atccagctaa cttttcagga aattaacttt aaagcaatcg
     7561 taggtcaaac tgatatttca gcagccactg gcaaaacggc taccacaaat gacggtggtg
     7621 tgaccagtaa gaagaatgca ggtaacgtga ccactgaacg caataacgcg cctatgctat
     7681 cgtgcaaaga gttaatgaat attagcgatg tatccggtta cgctccagct atcagaaatg
     7741 atattctcga tgctcagatt aaatgtaata aggcttggaa gctggactat tcagacttaa
     7801 cagaagctga aaaaaccaac gtcaccaatg ctaatagtaa cgctggtcga gtagcttcaa
     7861 agcttaaaaa gtactcagt
//
LOCUS       DAJTZL010000174         7803 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.174, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000174 DAJTZL010000000
VERSION     DAJTZL010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7803
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.174"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..1341
                     /gene="yjeM"
                     /locus_tag="OO544_003450"
     CDS             <1..1341
                     /gene="yjeM"
                     /locus_tag="OO544_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HCR6768969.1"
                     /translation="LMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIW
                     MVSTSAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKI
                     ARITAVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVV
                     FAIFAYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVL
                     SNGSVNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFT
                     LCYSPLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASA
                     FFNKLTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIAMVVVVLVVT
                     FANVFTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
     gene            complement(1492..4815)
                     /gene="mscM"
                     /locus_tag="OO544_003451"
     CDS             complement(1492..4815)
                     /gene="mscM"
                     /locus_tag="OO544_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HCR6768970.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNMRDKPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(4837..5805)
                     /gene="psd"
                     /locus_tag="OO544_003452"
     CDS             complement(4837..5805)
                     /gene="psd"
                     /locus_tag="OO544_003452"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HCR6768971.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKICQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            complement(5902..6954)
                     /gene="rsgA"
                     /locus_tag="OO544_003453"
     CDS             complement(5902..6954)
                     /gene="rsgA"
                     /locus_tag="OO544_003453"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HCR6768972.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            7049..7594
                     /gene="orn"
                     /locus_tag="OO544_003454"
     CDS             7049..7594
                     /gene="orn"
                     /locus_tag="OO544_003454"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HCR6768973.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
BASE COUNT         1782 a         2028 c         2096 g         1897 t
ORIGIN      
        1 ttaatgatgg ctgaaatggg agctgcttat cgcaaagaag aaggtggtat ctattcctgg
       61 atgaataata gtgtcggacc acgttttgcc ttcattggta cgtttatgtg gttttcctct
      121 tatatcatct ggatggtgag tacctccgcg aaagtttggg taccgttctc aacattcctc
      181 tatggtagcg acatgaccca gcactggcgt attgccggac tggagcctac gcaggtggtt
      241 ggtctgctgg cagtagcatg gatgattctg gtcaccgtcg ttgcttcaaa ggggattaat
      301 aaaattgccc gcattactgc ggtgggcggt attgcagtaa tgtgtctgaa tttagtattg
      361 ctgttagtaa gcattactat tttgttatta aatggtgggc atttcgcgca ggatattaat
      421 ttccttgcat caccgaaccc gggttatcag tccggtctgg caatgctatc gtttgtggta
      481 tttgctattt ttgcctatgg cggaattgaa gcggttggtg gtctggtcga taaaacggaa
      541 aatccagaaa agaactttgc caaaggtatt gtttttgccg ctattgttat ttcaatcggt
      601 tattcgctgg caatattttt atggggcgtc agcacaaact ggcagcaggt attaagtaat
      661 ggttccgtta accttggcaa tattacctat gtgctgatga agagcctcgg ggtgacgctg
      721 ggtaacgcac tgcatttgtc acctgaagcg tcattgtcgc tgggcgtatg gtttgcgcgt
      781 attaccggac tttcgatgtt cctcgcttat accggtgcgt tctttacgct ttgctattca
      841 ccgctgaaag ccatcatcca ggggacgccg aaagcgttgt ggccggaacc gatgacgcgc
      901 ctgaatgcga tggggatgcc gtctatcgcc atgtggatgc agtgcgggtt ggttactgtc
      961 ttcatcctgc tggtttcgtt tggtggcggt accgcatccg cgttctttaa caagctgacg
     1021 ctgatggcga acgtgtctat gacgcttcct tacctgttcc tcgcgctggc tttcccattc
     1081 tttaaagcac gtcaggatct cgacagacca tttgtgattt tcaaaacgcg tatgtcggca
     1141 atgattgcga tggtggttgt cgtactggtg gtgacatttg cgaacgtctt caccattatt
     1201 cagcctgtgg ttgaagccgg agactgggac agcacattgt ggatgattgg cggccctgtc
     1261 ttcttctcgc tgttagcgat ggcgatttac cagaactatt gcagtcgcat ggcgaataaa
     1321 cctgagttag ctctcgactg atcatatgaa atcctgcaac tcatcgtctc tccggcgatg
     1381 agttgtcagg aagtaatgcg tatggatgat actctccagg gccggtgata actgaagatg
     1441 atgttaagct gctaaatgag ccacgttgac atcatttggc agcttcatgc attacaaact
     1501 tcccgcctga cgacctttgc ccgcagacgt cagcgttctc cccgtttgtt taccgttgag
     1561 gctttccaga cgcatctgga agggcgggaa tggcatatcg ataccgtggg catggaagcc
     1621 agccagaatc agctggtgga tctcatggcg tagcggcata cggtgaccca tctcagcggc
     1681 ataaatacgc agctcgaaaa tctgaatccc ttgttgcaga tccaccagga agacttccgg
     1741 tgccgggttg tcgatcacca gcgagcagcg acgcgctgcg gtgagcagga tttccgtcac
     1801 ttcttcactg ttggcatcag caggggccgg aatcgtcaac actacgcgcg tgaccgagtc
     1861 agagagcgac cagttgataa actgctcggt aataaacgcc ttgttcggca cgattatctc
     1921 tttacggtcc cagtcgctga tggtggtggc gcgggtgtta attttcgtca cgctaccggt
     1981 gagatcgcga attgtcaccg tatcgccaat gcgaatcggt ttttcgaaca ggataatcag
     2041 accagagata aagttggcga aaatttcctg caaaccaaaa ccgagaccaa caccgagcgc
     2101 ggcgaccagc cactgcaatt tcgaccactc aataccaatc atcgagaagc cgaccagccc
     2161 gccaatcagc atcagcagat atttggtgat ggtggtgatg gcgtagcccg tacccggcgt
     2221 taaatccagg tgctgcaaaa tcgccagttc cagcagcgcg ggcaggttgc gcaccagctg
     2281 cgtggtgatg ataaacacca gaatggcaat cagcaccgca ccgagggtaa ttggctccag
     2341 actttctacg ccctgtaccg tggaggtgac atcccacagc gaaatatttt cgaggaagcc
     2401 gaaagcggaa tggatttctg accacagcac aatgacagaa agcagggcga tcaacatcaa
     2461 aattgagcgc accagccgca aggattgcgc actgatggca tcgagatcga cttcgctttc
     2521 atcgacttca attgctcctt ccgggctact gtgatgatgc gcttcctctt cgccacgcgc
     2581 acgttgcgct aacatctctg cccggcgatg cttcgcccgg tcaaacgcca gcctgcggcg
     2641 ctggatcagc atccagcggc ggataacgtg ataaaccacc agtagcagga accagatggc
     2701 aaccgaggtt tcaagcctcg ctaacagcgc ctgtgccgtt gccagataac ccacagccga
     2761 cgccagaatg gcaaccaacg gcgcgccaat catcatgttc cacagcatat ggttggtaat
     2821 gttgtcgccg ctgccttctt tgttgagata cagcggaatc ccggcctttt tcaggctgag
     2881 ggtgaccacc gccagcgcac cgcaaatgag gataaagcaa agccttccca gcgaaccgga
     2941 gaattcacgg tcgtcgaggt tatcgaacat catcagcgcc ataatcagcg gcacaataag
     3001 cccgatgctc atcaggtagt agcgcatccc acgagatacg cgttcgcgcg gccagccaaa
     3061 atgagcgata aacaagccgt tcgggcgggc aaaggtggcg caaatcatca ctacccacag
     3121 cagcggcacg gtggccgtta caccatcgcc aatcgcgacc gccagcggat aaggccacgc
     3181 ctcgcgcaag ccgtaaccca gcgtcatcca cagcaccggt aacggtgacg cgacgagaat
     3241 cgaccagaaa agagtgcgca acgtcagcca gaagtgatcc tgagtcactt tgccaacttt
     3301 cgccgccgaa cgttcaagaa aacgggtgaa atagcggcgc gagtaaatac tgcaaccgac
     3361 cagaatcaac gcgccaaaca gcggcaaaat tgtctctttg ctggtcagca tcatcacact
     3421 ggctttgccc aactgactga aggtgtccag cgaaatgaga cgacgcagat cctgggcgat
     3481 ttccagcggc caggcgatgg tcatcggacg cacgtcagag gtccagaaca gatagcggtg
     3541 cgttgcttcg ttcacctctt tcagcgcatc ctccagttgc ccgttggaga ctttcagctt
     3601 ggtcagttcc aatagtagcg tgtcgccacc ctgcaacaat gagttcagca actcacgctg
     3661 agtgcgcagt tgtgcttcca gaatacggtt ttgctcggcg gtcagcggct gaccgtcggc
     3721 ctgatggatt tgccgtagca gcggctgttt attaagcaga tcctcataac gtaaccgttg
     3781 cacacgcaac tgcgccattt cggtatcaag ctgttgtggt tttggcattt ctggtagccg
     3841 tgccacctgc gctcgcaacg cttcaccgag cagattggac gatcccagcc attgcgactg
     3901 ttcacgcagc gtattcaacg cctgccggac ctgtaacgtc tggctggcag cctgacgctg
     3961 ttgcgaggca acgagatcca tccgctgcgc ctgttgattc agcgctgccg atagttcgcg
     4021 gttaattttg aattgcgcga cgatatcttt cggcaaatcg gcactgtttt ctgccagtaa
     4081 ttcggtactt tccagcgccc gctccgcctc aagctgacgt tggctattca actgattacg
     4141 taaggcctgc aaatacgcat ccagttgctg gctctctttt tccgcaagct ctgagcgtaa
     4201 gcgcgctaat tcttggcggt tattggcaga cagctgcgcc agctccagtt catcaacgag
     4261 cgccttaaga cgtgcagagt cagactgcaa cgcgaaattt tgtgcctgat tgagcggagt
     4321 attgccggta agcgttccca ggcggcgctc gatctcattt aactgacggc gggcgtcggt
     4381 ttgctgttgc ggcagttgat tcagcgaatc ggcaatctcg cgggcgcgct cctgctcttg
     4441 ctgggcctga cggcttttat ccagcaactg gctgctgacc tggagaattt cctgattcag
     4501 cgcgtcggta gacattcccg gcgacacgct gcgcggcttg tcacgcatgt tgtttaattg
     4561 tgcgcgcaga gtagcggaga gtttcggata attatcgata acttgctgat attgtttgat
     4621 gcgctcaagg gaaccttttc gttcctcaag cgcatttaag gcagactgaa gcgcctctac
     4681 gacttccggc tgtgcgggtt tcgccgcttt tgcctgctcc agttcctgag tgatttgttt
     4741 gctatcgggg gccgtcgcgg cgtacgcccc ccaactgagg caccaggcca tcagaaaagt
     4801 gataatcagg cgcacgtcag cgtttccttt gatggattag acctggtctt ttttgtcgtc
     4861 aaccaatggg ctggcgtcgt gttctgcttc gatctcttca gcaggaagcg gggcaggttc
     4921 agcgtctggc gtaacaaagg tttcggtaga tactgccagc ggctggcaaa ttttcgtgac
     4981 agacaggctt tccagttgct caaccagatt cactttaccc ggtgcaaaca ggttgataac
     5041 ggtggaaccg agtttaaagc gacccatttc ctggcctttc agcagtgcca cagaaccgtc
     5101 gttttccccg gcaggccagg tccagcgctt gatgatacct tcgcgcggcg gcgtaatggt
     5161 gcccgcccag accgtctcaa tgctgccaac aatcgtcgct ccgaccagaa tctgcgccat
     5221 tgggccaaat tcggtatcga aaaggcaaat cacgcgttcg ttacgggcaa acagattcgg
     5281 cacgttctga gccgtgagat ggttaacgga gaagagatcg cccggcacgt agatcatctc
     5341 acgcagaata ccgttgcacg gcatgtgtac gcggtggtag tcacgcgggg agaggtaagt
     5401 ggtcacaaac gtaccgttgc ggaacaggtc cgccatcaga tagttgcctg ccagcagggc
     5461 ttcgaggctg tagttgtggc ctttggcttg caggatttta tcttcttcga ttttacccaa
     5521 ctggctgata acgccatcgg caggcatgac cagtacattc ggatcggtat cgattgggcg
     5581 tacttcgtca cgcagcggac ggacaaagaa ttcgttaaag gtgcggtagc tggcggtgtc
     5641 cggcttttgc gcctctttca tgtcgacctt gtagtattta acgaacagat cgataaccag
     5701 ttttgtcaac catcctgccc gcttgcttgc gccccaaccc gccaggcgag taagccatag
     5761 tttcggcaga atgtactgta gcgaaagttt aaatgaattt aacaaggtag cctccaggcc
     5821 attgttttgt cgttcctgat tcggcctaca tgccggatcc tgaaaaaaag gggacgattc
     5881 taacgacggt tagcttaatt gtcagtcatc cgtatcagaa aagtttttac gcgtttttac
     5941 ctgcgccatg ctttccagaa tacggtgata gttttcgaaa cgggtttccg cgattttccc
     6001 ttcctcaacc gcttcccgga tagcgcagcc cggatcggta tcgtgtttgc aatcgcgata
     6061 tttacacaga cctaaatagt catggaattc gacaaagccc tgagtgattt gttccggctc
     6121 caggtgccag aggccgaact cacgcacacc aggggaatca atcacatcac cgccgtgcgg
     6181 gaagtgatac agccgagcgg cggtcgtggt gtgctgacca agccccgagt tgtcagagac
     6241 atcgttggtc aggatctctt tttgcagccc cagcaacgcg ttcagcaggc tggatttgcc
     6301 gacgccagac tgcccggcaa aaatgctgat gcgcccggtc aacgcctctt ccagcggttt
     6361 tagcccatcc tgagtatggc tggaaaccat caatacgcga taaccgatat tgcggtagat
     6421 atccatctgc tcgttgacga acgccatgcc ttcgtcgtcc agcaggtcta tcttgttgag
     6481 cacaataatc ggctcaatct gcaaggtttc gcaggccact aggtaacggt cgataatatt
     6541 gagcgacagc tccggcaaaa tggcggagac aatgacaatc tggtcgatgt tggcggcaat
     6601 aggttttacg ccgtcgtaga aatccggacg cgtcaacacc gaggtacgct catgcaccgc
     6661 ttccacgatc cctttgacat ttacgccttc cgccgccggt ttacccggac gccagactac
     6721 gcggtcgccg gttaccagcg aacggatggt acggcgaata ttgcagcggt gaacgtcgcc
     6781 atcggcggat tccacatcag cgtgcatacc aaagcggctg atgacgatac cttcatcagg
     6841 ctcgccaaac agattgtcgt cgtagtcggg cttctcctta gacgttttaa gacgacgctg
     6901 gtgattggcg ttcacgcggc gctgctggcc tttggagagt ttatttttac tcaatcgtac
     6961 tgactcctgg tcgcccctga tgggcaaaac atctatgata cacgcaattg tggatcaata
     7021 tagtcactgt gaatgggtgg aaaatagcat gagtgccaat gaaaacaacc tgatttggat
     7081 cgatcttgag atgaccggtc tggatcccga gcgcgatcgc attattgaga ttgccacgct
     7141 ggtgaccgat gccaatctga atattctggc agaagggccg accattgcag tacatcagtc
     7201 cgatgaacag ctggcgctga tggatgactg gaacgtgcgc acccataccg ccagcgggct
     7261 ggtagagcgc gtgaaagcga gcacgatggg cgatcgggaa gctgaactgg caacgctcga
     7321 attcttaaaa cagtgggtgc ctgcgggaaa atcgccgatt tgcggtaaca gcatcggaca
     7381 ggaccgtcgt ttcctgttta aatacatgcc ggagctggaa gcctacttcc actaccgtta
     7441 tctcgatgtc agcaccctga aagagctggc gcgccgctgg aagccggaaa ttctggatgg
     7501 ttttaccaag caggggacgc atcaggcaat ggatgatatc cgtgaatcgg tggcggagct
     7561 ggcttactac cgcgagcatt ttatcaagct gtaaaatttt tcctggtcac gtaagcgccg
     7621 gaatccactt gcggcggcgt gattttgacg ctaaattgaa cgtcttgctg ataatttcag
     7681 cacttgagat aaaaacgcaa aaaaaacttt tttggggggt tgcagaggga aagatttctc
     7741 gtataatgcg cctcccgtaa cgacgcagaa atgcgaaaat tacgaaagca aaattaagta
     7801 gta
//
LOCUS       DAJTZL010000175         7777 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.175, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000175 DAJTZL010000000
VERSION     DAJTZL010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7777
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.175"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..1780)
                     /locus_tag="OO544_003455"
     CDS             complement(<1..1780)
                     /locus_tag="OO544_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010349194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HCR6768974.1"
                     /translation="MEKHGAELLLQRMLSNTSATFREGQWEAIDAVVNQRRKLLVVQR
                     TGWGKSAVYFIASKIFRDRGAGPTIIISPLLALMRNQVAAAERLGITAETLNSTNREE
                     WQRISDKLLRGGVDCLLISPERLANQDFLETANTPVLGTTATANNRVVEDIRQQLGDI
                     VIQRGTLARESLALDALVLGEQSSRLAWLATVIPQFSKSGIVYTLTTRDAELVAEWLR
                     TNGISAFAYYSGVTCEGAEDSNTAREYLEQALLANKIKVLVATTALGMGFDKPDLGFV
                     IHYQMPGSIVGYYQQVGRAGRAIDSAVGILLCGGEDRAIHKFFRESAFPAEAQIHEIL
                     NVLSENDGLTLRGIEQRTNLRYGQIEKALKLLVAENPSPVVYTEKLWRRTIVSFSPDH
                     ERINHLMNQRKNELADVESYITTKECKMQFLRRALDEPSAERCGKCSSCLQHPLLSPD
                     IDSGLLHAANLFIKHADLPLNLNKQVASGAFTQYGFKGNLPAGLQGSTGRVLSRWGDS
                     GWGKQVAQEKKTGRFSDELVEACAEMVRQRWNPHPEPTWVCCVPSLKHLDLVPDFARR
                     LAAKLGLPFIDAIEKVVDNPPQKMQQN"
     gene            complement(1921..3261)
                     /gene="gntU"
                     /locus_tag="OO544_003456"
     CDS             complement(1921..3261)
                     /gene="gntU"
                     /locus_tag="OO544_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HCR6768975.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTDTNLVKLIIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDINEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNNSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(3265..3792)
                     /gene="gntK"
                     /locus_tag="OO544_003457"
     CDS             complement(3265..3792)
                     /gene="gntK"
                     /locus_tag="OO544_003457"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HCR6768976.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(3931..4926)
                     /gene="gntR"
                     /locus_tag="OO544_003458"
     CDS             complement(3931..4926)
                     /gene="gntR"
                     /locus_tag="OO544_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HCR6768977.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHCHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(5150..5845)
                     /gene="yhhW"
                     /locus_tag="OO544_003459"
     CDS             complement(5150..5845)
                     /gene="yhhW"
                     /locus_tag="OO544_003459"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HCR6768978.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(5968..7005)
                     /locus_tag="OO544_003460"
     CDS             complement(5968..7005)
                     /locus_tag="OO544_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR6768979.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYILNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDICSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAK"
     gene            7339..>7777
                     /gene="yhhY"
                     /locus_tag="OO544_003461"
     CDS             7339..>7777
                     /gene="yhhY"
                     /locus_tag="OO544_003461"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR6768980.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQHPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKYGFEIEGTGKKYA"
BASE COUNT         1977 a         2101 c         1872 g         1827 t
ORIGIN      
        1 ggttttgctg cattttctgc ggtggattgt ccacgacttt ttcaatggca tcaataaaag
       61 gtaagccaag tttcgccgcc agtcgccggg caaaatcagg aaccaggtcg aggtgcttta
      121 atgaaggaac gcagcatacc caggttggtt caggatgcgg attccagcgt tggcgaacca
      181 tttccgcaca tgcctccacc agctcatcac tgaatcgccc cgtttttttc tcctgtgcta
      241 cctgctttcc ccacccggaa tctccccaac gagaaaggac tcgccccgta gatccttgta
      301 aacccgcagg caagttccct ttaaatccgt attgcgtaaa agctccagat gctacctgtt
      361 tattgagatt taatggcagg tcagcgtgtt taataaataa atttgccgca tggagtaagc
      421 cgctatcaat atcgggcgac aacaacggat gctgaagaca actgctacat ttaccacaac
      481 gttcggcact tggctcatcg agtgcgcggc gcagaaattg cattttgcac tccttggtcg
      541 tgatatagct ttcaacatct gccagctcat tttttctctg attcatcaaa tggttaattc
      601 gttcatggtc aggagagaaa ctgacgatag ttctgcgcca taatttctcg gtatacacca
      661 caggcgatgg attttcagct accaataatt tcagtgcttt ttctatttgc ccataacgga
      721 gattcgtccg ttgttcaatg cctcgtaggg taagaccgtc attctcgcta agtacgttga
      781 gtatttcatg aatttgcgcc tccgcaggaa aggcgctttc acggaagaat ttatgaatag
      841 cgcggtcttc accaccacaa agcaatatgc caactgcgga atctatagca cgcccggcac
      901 gccctacctg ttggtagtaa ccgacaatag accccggcat ttgataatga atgacgaaac
      961 ctaaatcagg tttatcaaac cccataccta atgccgtcgt cgcgaccagc actttgattt
     1021 tatttgccag cagcgcctgc tccagatatt ccctggcagt attagaatct tccgcgcctt
     1081 cacaagtcac gccgctgtag taagcaaatg cactgatacc atttgtcctt aaccactcgg
     1141 cgacaagttc ggcatcgcga gtagtcaggg tataaacaat tcccgatttg gaaaactgag
     1201 ggataaccgt tgctaaccat gccagacggg atgactgttc acctaagact aaggcatcaa
     1261 gcgccaaact ttcgcgagcc agcgttccac gctgaatcac aatgtcaccc aattgctgac
     1321 ggatatcctc aacgacacga ttattcgctg tcgcggttgt acccagaaca ggggtattcg
     1381 cagtttcaag aaaatcctga ttcgccaacc gttcaggtga aatcagcaga caatctacac
     1441 caccccgcag caacttatcg ctaatgcgct gccattcttc cctgtttgta gaattcaacg
     1501 tttcagccgt aatacctaac cgttcagcgg cggcaacctg gttacgcatc agggcaagca
     1561 aaggagagat gataattgtc gggccagcgc cgcgatcgcg gaagatttta ctggcgatga
     1621 agtacacggc acttttcccc cagccagtgc gttggacaac cagcagtttt cgccgctgat
     1681 taaccaccgc atctatagct tcccattgcc cttccctgaa agtagcgctg gtgttactta
     1741 acatccgctg taacagcaat tcagctccgt gtttctccat catcttctcc ctcgtccgtt
     1801 aacgcagtga ttagaacgga taatgttccg gtctttttgc gagagagatc aaaaagatga
     1861 agacaggaga taagagaaat gaattactgc gagcaagctc gcagtaattt gtcgcagtaa
     1921 ttaactcaac agctggaacg caatcatccc aacaatggca ccaacagtgc cgaggatggt
     1981 ttccatcatg gtccaggttt tcagcgtttc ggcttcggtc gcgccggtaa atttaccgaa
     2041 caaccagaaa ccagcgtcgt taacgtggct gacaacaatc gaaccaccgg cgatacaaat
     2101 cgacagcgcc gccatttgcg caccggaatt gttcagttgt tcaatgaccg gcatcaccag
     2161 ccccaccgcc gttaaacagg caacggtggc agacccctga atgatgcgca ctgcagctgc
     2221 cagcacgaag caggtgatag caatcggcag gcccatgccg gttaacgctt cgcccagtgc
     2281 cggacctacg ccagagtcaa ccagcacctg tttgaacacg ccgcctgcac caatcaccag
     2341 cagaatgatc cccgccggtt gcagcgcgtg accgcaaatc tccatcactt tgtcttttgg
     2401 catgccctga cgcattgcca ggccataaat cgctaccaga caagcaacca gaatcgcggt
     2461 aaacggatga ccaataaact cgaaccattc gtaagcggta gagccttctg gcacaaaacg
     2521 cgcggcaatg gttttcagcc ccaccagcac cagcggcaac aggatcagcg acaggctgaa
     2581 tccgaaagat ggcattttac cttcgccgag atgcggctcg ttgatgtcgt caggaatatg
     2641 cagctcaacg taacggctga tgaagttacc ccacagcggc ccggcaataa tcattcccgg
     2701 aattgccgca cacaggccaa tcaggatcat ccagccaaaa tcggcgttca tctgcgatgc
     2761 cagcagcatt ggcgctggcc ctggcaccag gaacgccgcc gctgccgcca cgcctgcgaa
     2821 taatgggatt atcagcttca ccaggttcgt atcggtgtgg cgcgccattg agaaagcaac
     2881 gctaatcagc agaacaatcg ccacttcaaa gaacagcggt agcgcacaga ccagccccgc
     2941 aaggccgatg gcataatgcg cgcggctgtg accgaaggat ttgagcattt tgacggcaat
     3001 ctgatcgact gcgccggttt catgtaagat cttgccaaac atagctccca gggcgacaac
     3061 caccgccagg aagccgaggg tgcctcccat ccctttttcc atcgtcgctg cgattttatc
     3121 gagcggcata ccggaaaaaa ggccagcccc catggacacc accatcaaag ccaggaaagc
     3181 gtgcatacgc gccttcatga ctaaaaacag cagcagtaaa acagacccta ctgctgttaa
     3241 aacaagcgtt aatgtagtca ctacttattt gcctttttta ataacctcaa tggtgcttgc
     3301 cacaacacct tccagcggtt gatcgatatc caccaccagt acatcggttt cgtccgcacc
     3361 cggctcctgc agcgtttcaa actgcgtcac caacatttgg gttttaaaga aatggccttt
     3421 gcgcgctttc aggcggcttt caatcacgtc aaaatcgcct ttcaggtaga tgaaagagag
     3481 attcggatta ccttcacgca gcaagtcgcg atagtgtttt ttcaatgcag aacagacgat
     3541 cagtgacact ttattagtgc gctgcatagc aaacgcggcg tcgttcagcg cctgcaacca
     3601 gggtttgcga tcgtcgtcat tcagcggttc gccagacgcc attttttcga tattgcgccg
     3661 tggatggagg aaatcgccat caagaaacgc ggcatgaagt tgatgcgcca cttcactggc
     3721 gaccgcagat ttgccgctgc ccgatacgcc catcaagacg taaatgtggt gatcatggtt
     3781 agtcgtgctc aaagtggtgc ccccacaata caagaattaa aaatgttacg ggtaactgtt
     3841 atcggtaaca ttgtccagcc ggacaatatc agaagcaata tccatccgtg ccttaagtgt
     3901 ataagtgtga gctacttcaa atttgtaggc ttaaatagat ccgcccggtg acaaggtgaa
     3961 acctaaatct aacattttcg gtgtcacaga ttcgccacga atacgcgcca gcaggcgttc
     4021 agcgccaata ctgcccatcc gctcacgcgg cgtcagcacg ctcgctaacc gcggctccat
     4081 cacctggcca atgtcatgac cgtggaaacc ggcaatcgcc atatcgtcag gaaccttcaa
     4141 tcccagacgc tgacattcaa acgccgcgcc gaccgccagg tcatcgttgg tacagaacac
     4201 gccatccagc tgcggatatt cccgccgcgc ctggcgaatc agttcaatac cggaagagta
     4261 agaagaagat tgctcaacca tcacgctata cggcaccaga cctgcatcca gcatcgcctg
     4321 ttcgtatccc ttctgtttga tgatagtacg ttcgtcgaga cgcgcgccga gataggcaat
     4381 gtggcaatgc ccgcgagcaa taatggcggt ggtcatctgg cgtgccgctt caaagttatc
     4441 aaaaccgacg gcgatatcga ggcacggcga ctggctgtcc atgagttcca ccaccgggat
     4501 ccccgccact tcaatcatct ttaaggtgcg cggcgtgtgg gtacgttcgg tgaggatcag
     4561 tccgtcgata ttccaggaga gcatggattc gagacgttct tgctccattt ccggtttata
     4621 accgtagtga gccagcatgg tctgataacc gtgcgcgtcg gtgacgcttt cgattccgcg
     4681 taatacttcc gcgaaaacct ggttggtgag agaaggtaac aggacgccaa tcgcccggct
     4741 ggtggcgtta gagaggatat cgggcgcacg attgggaata tagcccagtt catcaagagc
     4801 ggcggcaatc ttgccgcgta gagcgacgga aacctgctcc gggttgcgta aaaaacggct
     4861 gaccgtcatt ttggtcacgc ctacacggtc agccacatcc tgaagtacgg gtcttttctt
     4921 tttcatcgtc ctgaaggtac aaaagagata gattctctta gtttaacacg gacgcatcag
     4981 aaccttcccg gggaaaacag tgcttgttta caattattag atatacacta aatcaattgc
     5041 atcgcattgt gctaacgacg cggtaaaacc ccggaagcgt gcatcagcac gcgaccggag
     5101 cgagcgagga agcctgcaaa aaggcaggct tcaaagatgc cgttgagttt taaaccggcg
     5161 gcagatcgaa cagtaacact tcgctgtcgc tatccgcatg gatggagatt gcctgctcat
     5221 cccagattgc cagaccatcg ctggtcgagg ctttcacgcc gttaatggtg acattgcctt
     5281 tcaccacctg gatccagacg cggcgttcag cggcaatctg atgcaccgac tgctcatctt
     5341 tcagcaacgc ccagcggtac agttccatat cctgatgcac tttcaatgaa ccatctcgcg
     5401 catccggcga gagcaccagc tgtttgccct gtacggcatc gaagcgacgc tgttcataac
     5461 gcggcgtaat accgttttct tcaggcatga tccagatctg atacagatgc agacgctcgg
     5521 tgctgcttgg gttgtactct gagtgacgaa tacccgtacc cgcactcata atctggaact
     5581 cacccgccgg aacctgctct ttattgccca tgctgtcctg atgctcaaca gtaccttcca
     5641 gcacgtaggt caaaatttcc atatctttat gcgggtgagt gccgaagccc tgccctgctt
     5701 caatcacgtc gtcgttaatc acgcgcagcg cggagaagcc cataaagttc ggatcgtaat
     5761 agttggcaaa agagaaagta tgccaggagt ccagccagcc atgatttgca tgaccacgtt
     5821 catttgcttt gcgtaagtag atcatttgta ttcgccccat gaatgatttc gatgggctta
     5881 gtgtggaccc gatctgcctg ggatgataga gggtgaaaat tgacccctct gttcaaaaaa
     5941 attgaactat tcgaggggcc atcaaactta cttcgcgaga gtcactgtgg agggagaggc
     6001 ttgctcaaat ccgcgttcaa ggatttccag attggtaaga acttcagatt ccttgacgta
     6061 atttggcgca ccgtgggtga tggtttgata caacgcatca taaacgcgcc cgtaatcgcc
     6121 catctccggc ttcatctctt ctctgaccgt cacgccctcg tcattgacat actccagcac
     6181 accgaccgaa tcatccgctg cgaatcccgg ttcgcccggc ataatattag ccttcaggct
     6241 ggtttcctgc tggtcgatac cgtatttaat aaacgaacct ttcttaccgt gaacgataaa
     6301 tttcggataa tcgattttca ccagatggct ggttttgacg atggctttca ggtcgccata
     6361 aaacagttgc gcttcaaagg tgtcgtcagg attggcttta ttacgcaggc tgcagatgtc
     6421 ataagcgacg tgatccgggc gaccgaacag agaaataatc tggtccatcg tatgcacacc
     6481 aaggccatag aacgcgccat cctgcggcaa cccaggtttg gtttctgcca ccgggcggta
     6541 atagtcaaaa tggctttcca cttcaacaat ctctcccagc ttaccgcttt caatcgcttt
     6601 tttcgccgtc aggaagcagg aatcaaagcg acgattctga tatggcgtga cggtcagccc
     6661 tttgcttttc gccagtgcaa acagctcttt cgcctgcgca agtgtcggag tgaacggttt
     6721 ttcgaccagc acatttttcc cggcttccag cgcgcgcttc gcgtactcga agtggctgtc
     6781 cgcatgggtg cagacaacaa ccaacttaac atcgggatcg tttagcactt cgtcgagatc
     6841 gctggtgaaa tggatatgag agtaaatggg agcctgttct tccggcttcg catggcgacg
     6901 aaaaatatgc gcgacatgcc agctatcctt gcggttaagt atatacggca gatggtaacg
     6961 ggtggtgctt ttgccgaagc caataaaggc gcagttgatg accatgattt cgtccttttt
     7021 aaggtggtta tttacacctt agcgcaaagc ggacgtggtt cctactggag tgcgcataac
     7081 gaacacaagc actcccgtgg ataaattgag aacgaaagat caaaaaaaaa gccagcaccc
     7141 ggctggctaa gtaatactgg aagcaatgtg agcaatgtcg tgctttcagg ttctccgcga
     7201 gggtcttcct gatcgcgaga caataataat cattctcatt cgcacttgtc caacactttt
     7261 tgcaaaaaaa atgcatttga ctcgcatttg aaagtcaatg atgttgaaag ggacatttac
     7321 cccaaagagg acaaaggaat gagtgagata gtaatacgcc acgcagaaac acgggattac
     7381 gaggccatca ggcagattca cgcccagccg gaggtgtatt gcaacacact acaggtgcct
     7441 catccttccg atcatatgtg gcaggagcga ctcgccgatc gtcccggcat caagcaactc
     7501 gtcgcctgta ttgatggaga cgtcgtgggc catctcacca ttgacgtgca acagcaccca
     7561 cgccgcagtc atgttgccga ttttggtatc tgtgtcgact ctcgttggaa gaaccgcggc
     7621 gtcgccagcg ccctgatgcg agagatgatt gaaatgtgcg acaactggtt gcgggtagat
     7681 cgcattgaac taaccgtgtt tgtcgataac gccccggcaa ttaaggtcta taaaaaatac
     7741 ggctttgaaa ttgaagggac tggtaagaag tacgcat
//
LOCUS       DAJTZL010000176         7739 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.176, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000176 DAJTZL010000000
VERSION     DAJTZL010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7739
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.176"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            18..515
                     /locus_tag="OO544_003462"
     CDS             18..515
                     /locus_tag="OO544_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HCR6768981.1"
                     /translation="MSPSLRKAVAVAIGGGAVAIASVLITGPGGNDGLEGVSYILYKD
                     IIGVWTVCHGHTGKDIMPGKTYTEAECKALLNKDLATVARQINPYIKVDIPETTRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGYIKGACDQLCRWTYAGGKQWKGLMTRREIEREVC
                     LWGQQ"
     gene            512..982
                     /locus_tag="OO544_003463"
     CDS             512..982
                     /locus_tag="OO544_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005013949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HCR6768982.1"
                     /translation="MSRVTAIISALVICIIVRLSWAVNHYRDNAITYKEQRDKNAREL
                     KLANATITDMQQRQRDADALDAKYTKELADAKAENDALRRKLDNGGRVLVKGKCPVPS
                     SAETSSASGMGNDATVELSPVAGRNVLGIRDGIISDQTALRMLQEYIRIQCLGG"
     gene            complement(1071..1583)
                     /locus_tag="OO544_003464"
     CDS             complement(1071..1583)
                     /locus_tag="OO544_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HCR6768983.1"
                     /translation="MMEWINALMNYFVLHPYRLWGLLFIIAFTKSTLLISAILPPASI
                     MMATVITVSKTTLSPWEAGITVMSGAWCGTIVNYHLGIIIGHIPQLACIISSRSNTIE
                     RVRLQLQNNSVSILFTSRFIAVLRYIAPLVAGMLQLHPVKVYTVSLISAASWSALYVG
                     SFSFVLPFFS"
     gene            1778..2542
                     /locus_tag="OO544_003465"
     CDS             1778..2542
                     /locus_tag="OO544_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6768984.1"
                     /translation="MAKPDWEAIESAYRAGVLSLRDIGEKYGVTEGAIRKRAKKLGWA
                     RSGGTQVCKNGTQKRKVRTSRKPAITGLTQKSTQLKTESTPDTKPIRGMRTDPPTNPF
                     QPGNQQALKHGGYARRLLLKDEVIEDAKALTLEDELFRLRANNLVAAENIGRWLVSLE
                     DANGDQERKMLIENISAAEKAMMRNTVRIESIVGTLATVGKIFADTAYRKAATDKVSL
                     EADRLRRDAGIDDGNGERDLNDFYSDIQTDAESGFT"
     gene            2493..3896
                     /locus_tag="OO544_003466"
     CDS             2493..3896
                     /locus_tag="OO544_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HCR6768985.1"
                     /translation="MTSTLTSKPTLNPALRSFWTMRARNKVLYGGRSSSKSWDAAGIA
                     IFLSNKYTLRFCCARQIQNKIEESVYTLLKIQIDRFGLRHRFRILNNKIINRVTGSEF
                     VFYGLWRNIEEIKSLEGIDVLWLEEAHALTEYQWKILEPTIRKEGSECWFIFNPGLVT
                     DFVWRNFVVDPPEGTLIRKINYDENPFLSDTMLKVIDAARRRDPDGFKHVYEGVPESD
                     DDAAIIKLSWIEAAVDAHKTLNFEPSGRKRIGFDVADSGTDKCANVYRHGSVVFWADE
                     WKAKEDELLKSCQRTYQAALEREADIVYDSIGVGASAGAKFSEINADRKSENAYARRV
                     NYQRFNAGAGVHEPDDEYNGIPNKDFFANLKAQAWWLVADRFRNTFNAINNGEQYPVD
                     ELISIDSRCPLLEKLKLELTTPHRDFDRNGRVMVESKKDLAKREIPSPNVADAFIMAF
                     APIDTSLDIWEQLGRQA"
     gene            3896..5362
                     /locus_tag="OO544_003467"
                     /pseudo
     CDS             3896..5362
                     /locus_tag="OO544_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706598.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
     gene            5253..5996
                     /locus_tag="OO544_003468"
     CDS             5253..5996
                     /locus_tag="OO544_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HCR6768986.1"
                     /translation="MTNRRSRQAEIRYRTSLRRIARAVGDIVNGRYDGSNDSITEIIA
                     ALERYSEIITPWATKVAENFTADLTRQNEKVWRQHSKNISRELRNLVESAPVGQVMQS
                     IIAEQVKYIKSLPLEAADRVYDIQNKAIEAVVTGGRAEQFAKEIASTGDVAKSRADLI
                     ARTELGRATGALDMARAIAIGSDGYIWRTADDGDVRDSHDHMKGKFVRWDSPPTLDGM
                     TGHAGELPNCRCYKEIVFVRVPFAMKRAA"
     gene            6000..7220
                     /locus_tag="OO544_003469"
     CDS             6000..7220
                     /locus_tag="OO544_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HCR6768987.1"
                     /translation="MKYFFETRLGETRYRLADGSLLCKDVPIGRTGKQLYGADDLPKL
                     KPDKFGEIVVTRSPEQVFHPATLASFEGMSITILHPEDENGNVRLVNPENWKELAVGH
                     LQNVRRGTGEQSDLMLADLIVKDESAIQLIEDGLREVSCGYDAEYEQTEPGKAEQVDI
                     TGNHVALVPKGRAGNRCAIGDRDTMANQKKNWWNRMRAAIKTGDADTMNELVESAPAS
                     VTGDEGDLPQGVNLNINLSPQQPLPDKAPEMGGDPTGDSDDDLKTLLKALLAKLERNA
                     TGDNDNKPDDNPTGDGEDDEEETTITGDSAWRAEVIVPGIDLSRKMKPTAFKREVLAS
                     ADKTLVRQIVGDADIRKLPKQSVDMAFNAVSEVAKGRNTRATTGDAQRLNMGMTSIAS
                     LNKQNAEFWANRKG"
     gene            7224..7730
                     /locus_tag="OO544_003470"
     CDS             7224..7730
                     /locus_tag="OO544_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6768988.1"
                     /translation="MNNVFLYRMPVGIAGAVSRPQDLTVEPVVLKSDNAFAAYGLAGK
                     YDDDGFFVPLADGDTADKVKGIYVRPYPTTSQPDMVRQVGTGKNFPGDAMKRGYVTVN
                     LGSDFDASTIKKGDPVYVVVSTDESIKVPLGGFMSTSVSGKNVVLTNAEFTGAGDADG
                     NAEISWKI"
BASE COUNT         2059 a         1845 c         2109 g         1726 t
ORIGIN      
        1 gctgcgagag gtgaataatg tcgccatcat tacgcaaggc tgttgcagtt gctattggtg
       61 gcggggctgt tgctatagca tctgtgttaa tcactggccc aggtggtaac gatggtctgg
      121 aaggtgtcag ctacatacta tacaaagata tcattggtgt atggactgta tgtcacggac
      181 acaccggaaa agacatcatg cctggtaaaa cgtataccga agcagaatgc aaagctctcc
      241 tgaataaaga ccttgccact gtcgccagac aaattaaccc gtacatcaaa gtcgatatac
      301 cggaaacaac gcgcggcgct ctttactcgt tcgtttacaa cgtgggcgct ggcaatttca
      361 gaacatcgac gcttcttcgc aaaataaacc agggctatat caaaggcgca tgtgaccagc
      421 tatgtcgctg gacatatgct ggcggtaagc aatggaaagg gctgatgacc cgtcgtgaga
      481 ttgagcgtga agtctgtttg tgggggcagc aatgagcaga gtcaccgcga ttatctccgc
      541 tctggttatc tgcatcatcg tccgcctgtc atgggctgtt aatcattacc gtgataacgc
      601 cattacctac aaagagcagc gcgacaaaaa cgcaagagaa ctgaagctgg cgaacgcaac
      661 cattactgac atgcagcagc gccagcgtga tgctgatgca ctcgatgcta aatacacgaa
      721 ggagttagct gatgcgaaag ctgaaaatga tgctcttcgg cgcaagcttg ataatggtgg
      781 tcgggtgctc gtcaaaggaa aatgccctgt gccatcctca gccgaaacct ccagcgcctc
      841 cggcatgggc aatgatgcca ccgtcgaact ctctccagtt gctggacgaa acgttctcgg
      901 tatccgggac ggaatcatca gcgaccagac agcattgaga atgcttcagg agtacatcag
      961 gatccaatgc cttggggggt agcggtaatt ttactcatta tccttcaaat cagattctgt
     1021 tgtcagagga atgggggaag ctggattcgc aagagctttt tatgggcagt ttagctaaaa
     1081 aaaggcaaca cgaagctaaa actaccaaca taaagtgctg accagctagc agctgaaatc
     1141 agactcactg tatacacttt cactggatga agttgcaaca ttcctgccac caaaggagca
     1201 atataacgca gcacggcgat aaagcgcgaa gtgaaaagta ttgacacaga gttattttgc
     1261 aactgaaggc gaacccgttc tattgtattg gaacggcttg atattataca tgcaagttga
     1321 gggatatgcc caattattat accaagatgg taattaacga tagtaccgca ccatgcgcca
     1381 ctcatgaccg tgattccagc ttcccatggt gaaagagtcg tcttgctgac tgtaattaca
     1441 gtggccatca taattgatgc tggtggcaat atcgccgaaa tgagtaaagt ggattttgtg
     1501 aacgctatta taaaaagaag cccccatagg cgatatggat gaagtacaaa atagttcatc
     1561 aaagcattta tccactccat caatgtcttt ccttctgtga atttttcttt gctatttaac
     1621 cgtgatacga cttaatttag gcttaaacac cttacattta caaggtactc ctgtgggggg
     1681 ccctgccacg gagcgtcgca ggagtgagtg atgaggaaga attaaagcaa cctattgtga
     1741 caggcagcga taacagtaaa tcacaggaag cataatcatg gcaaaaccgg actgggaggc
     1801 catcgaatcg gcataccggg ccggagtcct tagcctccgt gatataggcg agaaatacgg
     1861 cgttacagaa ggggctatca ggaagagggc caaaaagctt ggttgggcac gcagtggcgg
     1921 tacgcaggtt tgcaaaaatg gtacgcaaaa aaggaaagtg cgtaccagca gaaagcctgc
     1981 cattactggc cttacacaaa aaagtacgca actaaaaaca gaatctacac cggatacgaa
     2041 accgatacgc ggaatgcgta ccgatccccc gactaaccca ttccaacccg gtaaccagca
     2101 ggcattaaaa cacggtggtt atgcccgtcg ccttctgctc aaagatgagg tgatagagga
     2161 cgctaaagcg ttgacgctcg aggacgaatt atttcgcctt cgtgctaaca accttgtcgc
     2221 tgcagagaat attggtcggt ggttggtgtc gctggaagat gctaatgggg accaggaaag
     2281 gaagatgctg attgaaaata tcagcgccgc cgagaaagca atgatgcgca atacagttcg
     2341 tattgagtcc atcgttggca cgcttgcgac ggtaggaaaa atatttgctg atacagccta
     2401 ccgcaaggcc gccactgata aggtgtctct ggaggctgat cgtcttcgcc gtgatgcagg
     2461 tattgatgat ggcaatggag agcgtgacct caatgacttc tactctgaca tccaaaccga
     2521 cgctgaatcc ggctttacgt agtttctgga ctatgcgggc acgtaacaaa gtgctttatg
     2581 gtggtcggtc atcgtcaaaa tcatgggatg ccgctggcat tgccatattt ctgtcgaata
     2641 aatacaccct gcgtttttgt tgtgcccgtc agatccagaa taaaatcgaa gagtcggtgt
     2701 ataccctgct caaaattcag atagacaggt ttggtctgcg gcaccgtttc cgtattctga
     2761 acaacaaaat cattaaccgg gttactggct cggaatttgt tttttatgga ttatggcgca
     2821 acatcgaaga aattaagtca ctggagggga tcgatgtgtt gtggctggaa gaagcccacg
     2881 cactgacgga ataccagtgg aaaattctgg agccaacgat ccgtaaagag ggttcggaat
     2941 gctggttcat attcaacccc ggacttgtta ctgatttcgt ctggcgcaac ttcgttgttg
     3001 atccgcccga aggcactctc atccgcaaaa ttaactatga cgaaaatccg tttctgtctg
     3061 acaccatgct taaggttatc gacgcggcgc gacgccgtga tccggatggt tttaaacatg
     3121 tgtatgaggg cgttccggag tctgatgatg atgcggcaat catcaaactg tcttggatag
     3181 aagccgcagt ggatgcgcac aaaacgttaa atttcgaacc cagtggaaga aagcgtattg
     3241 gctttgacgt ggctgacagt ggtacagata agtgcgctaa cgtttaccgt cacggatccg
     3301 ttgttttctg ggccgacgaa tggaaggcca aagaagatga attactgaag agctgccagc
     3361 gtacttatca ggcggcgctg gagcgtgaag cagatattgt ttacgactct atcggtgttg
     3421 gtgcgtctgc cggtgctaaa ttctctgaaa ttaacgctga ccggaagagc gagaacgcat
     3481 acgcgcgacg tgtgaattac cagaggttta acgccggtgc tggtgtgcat gagccagatg
     3541 acgagtacaa cggcatcccc aacaaagact ttttcgcaaa tcttaaggct caggcatggt
     3601 ggctggtggc tgaccgtttc agaaatacgt ttaacgccat taacaacgga gaacagtatc
     3661 ctgtggatga gctgatcagc atagattctc gttgtccgtt gcttgaaaag ctgaaactgg
     3721 aactgacaac acctcatcgt gatttcgacc gtaacggacg tgtgatggtc gaaagtaaaa
     3781 aagacctcgc aaaacgcgag ataccgtcac caaacgttgc tgatgcattc attatggcct
     3841 tcgcgccaat cgatacatcg ctggatatct gggaacagct ggggagacag gcctgatggc
     3901 acgaaacaaa caagccctgc ggcgaactgt gcaggccaca gctgatggtt atgagaattt
     3961 tattgcccgc gtagggatgc agacacctaa ccagcactca gcatccacct accgggctaa
     4021 tttcaccagt cgtaaccgca tgctggtgga atggtcctat cgttcatcct agatcatcgg
     4081 cgaagcagtc gatgctatcc cggatgatat gacccgcaaa ggcattcgca tcacttcgga
     4141 aattgatgca aaagatcgtg gcattctcga atcacaactg gatgagttgc aaatctggga
     4201 tgcgctgaat gacgtgctga aatggtcgcg cctctacggc ggcgcggtgg gtttcatcat
     4261 gattgagggg taggcaccaa tgaccccgct gcgacccgaa accatcggta agggcaagtt
     4321 taaggggatt ctcccgctcg accgctggat ggtcgacccg gcactgaccc gccgcattaa
     4381 agatatgggg ccggacctgg gtaaacctga gttttacgat gtggtgacca cagcaacggg
     4441 aattcctgcc tggcgcattc atcacagtcg cctgattcgc tttgatggcg tcacgttgcc
     4501 atttcagcag aagatgaccg agaacgaatg gggaatgtcg gttgtagagc gtatctggga
     4561 tcgtcttacc gcgttcgaca gcgctactgt cggcgcggcg cagctggtct acaaggcgca
     4621 tttgcgtacc tacagcgtgg agaagctacg cgagcttatc gcacttggtg gtcctgcgta
     4681 tgaagcgttg ctgaagaata tcgacctgat tcgacagttc cagagtaatg aaggcatgac
     4741 gctcatggac tcgcgggata agtttgaaac ccatcagtac agcttcagtg gtctggatga
     4801 catcctttcg cagtttgtag aacagattag tggcgctgtt ggtatcccac tggtgcggtt
     4861 gttcggacag tccccgaaag gattttctac cggtgatgca gaccttgcca actattacga
     4921 tcgcatcagt tcgttgcagg agaggcgttt acgtcttccg gtgcggcgga tactggacat
     4981 catgcatcgt tcggaacttg gcaagccgct gccggacgat ttcacgtttg agtttaaccc
     5041 gctctggcaa atgtctgatg tcgatcgctc aacggtggcg ttaaacacca ccaacgcaat
     5101 cagtacggcg ctgggtgatg gtctgatgac actgaaagcc gctatgactg atttgcgaga
     5161 aaattctgac gtaaccggca tcggggcatc cattaccgac gaggacatcg agaatgccga
     5221 agatgaagcg ccgcccggca tcggcgaacc tgatgacgaa ccgcaggagc cgtcaggcgg
     5281 aaatccggta tcgaaccagc ctacgcagga tagcgagggc ggtcggagac atcgtaaatg
     5341 gtcgctacga tggttcaaat gacagtatca cggaaattat tgcggcgctg gagcgttaca
     5401 gtgaaatcat caccccctgg gcgacaaagg tcgcggaaaa ctttactgcg gacctaaccc
     5461 ggcagaacga gaaagtttgg cggcaacaca gcaagaacat cagtcgcgag ctccgcaatc
     5521 ttgtggaaag cgctcctgtg ggccaggtga tgcaatccat catcgccgaa caggtcaagt
     5581 acatcaaatc tctgcctctc gaggccgcag acagggtgta cgacatccag aacaaagcga
     5641 tagaggctgt tgtcaccggt ggtcgggcgg agcagtttgc taaagagatt gcatctaccg
     5701 gcgatgttgc taagtctagg gccgatctga ttgcccgaac ggaactggga agagcaacgg
     5761 gcgcgctgga tatggcccga gcgatagcta ttggctcgga tggttatatt tggcgtacag
     5821 ccgatgatgg cgatgtccga gattcccacg atcacatgaa aggtaaattc gtccgctggg
     5881 attcacctcc aactttggac ggcatgaccg gccacgcggg cgagctgcca aactgccgct
     5941 gctataaaga gatcgtgttt gttcgcgttc cattcgcaat gaaaagggca gcataaccca
     6001 tgaaatactt ttttgagacc aggctcgggg aaacccgata ccgcctggct gacggctcgt
     6061 tgctgtgcaa agacgtgccg ataggacgaa caggtaagca gctctatggt gctgatgacc
     6121 tgccaaaact gaaacccgat aagttcggtg aaatagtcgt cacgcgttct cctgagcagg
     6181 tattccatcc ggccacgctt gcctcattcg aagggatgag catcacgatc ctgcatcctg
     6241 aagatgaaaa cgggaatgtg cggctggtaa atcccgagaa ctggaaagag cttgctgtcg
     6301 ggcacctcca gaatgtccgg cgcgggacgg gtgagcagtc tgatttgatg ctggctgacc
     6361 ttatcgtcaa agacgaaagc gccattcagc ttatcgaaga tggcctgcgc gaagtgtcgt
     6421 gcggctatga cgcggagtac gagcagaccg agccaggtaa agccgagcag gtcgatatta
     6481 ccggaaacca tgtggctctt gtccctaaag gcagagccgg aaatcgttgt gcaattggag
     6541 acagagacac aatggcaaat caaaagaaaa actggtggaa ccgcatgcgt gcggccatca
     6601 agacaggaga tgccgacacc atgaacgaac tggtggagtc ggctcccgca tcggttacag
     6661 gagatgaggg ggatttgccg cagggcgtta atctcaacat caacctgtcc ccgcagcaac
     6721 cactaccgga caaagcacca gagatgggcg gagatccaac cggcgacagt gatgatgacc
     6781 tcaaaacatt actgaaagcc ctgctggcta agctggaaag aaatgccacg ggcgataacg
     6841 ataataagcc tgacgataat ccgaccggtg acggcgagga cgatgaagag gaaaccacga
     6901 ttactggtga ctcagcctgg cgtgccgaag ttatcgttcc gggtatcgat ctgagccgta
     6961 agatgaaacc gaccgcgttc aaacgcgagg ttctggcttc tgctgacaaa acgctggttc
     7021 gccagatagt cggtgatgcg gatatccgca aattaccgaa acaatcggtc gacatggcgt
     7081 ttaatgccgt gtctgaggtt gccaaagggc gaaacacccg cgccaccacc ggcgatgcac
     7141 agcgcctaaa catgggcatg accagtatcg cttccctgaa caaacaaaac gctgaattct
     7201 gggcaaaccg taaagggtaa aaaatgaata atgtatttct gtaccggatg cctgttggca
     7261 ttgccggggc tgtctctcgc ccgcaggact taaccgtcga accggtggtc cttaaatccg
     7321 ataacgcctt cgctgcctat ggcctggctg gtaaatacga tgatgacggt tttttcgtgc
     7381 cgctggcaga tggtgatacc gcagacaagg tgaaggggat ctacgtgcgc ccttatccga
     7441 ccacgtcgca gccggacatg gttcgccagg tgggaacagg caagaacttc ccgggcgacg
     7501 ccatgaagcg tggctacgtg accgttaatc tcggttctga ttttgatgcc agcactatca
     7561 aaaaaggcga tccggtatac gttgtcgtct ccactgatga atccatcaaa gtgccgctgg
     7621 gtggattcat gtccacgtca gtcagtggca aaaacgtggt gctgaccaac gctgaattca
     7681 caggtgccgg tgatgctgac ggcaatgcag aaatttcctg gaagatttaa ggaacagac
//
LOCUS       DAJTZL010000177         7716 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.177, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000177 DAJTZL010000000
VERSION     DAJTZL010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7716
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.177"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..644
                     /locus_tag="OO544_003471"
     CDS             <1..644
                     /locus_tag="OO544_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6768989.1"
                     /translation="HAFITESDADGHVLTVREDLIWPLLEVLYPGTRETFGLPGICLS
                     LADKPDELAALEHYWQLIERESVEQLPGREHTLTLLAQAVFTLLLRNAKLDDHAASGM
                     RGELKLFQRFNMLIESHFHQHWTVPDYANELHITESRLTDICRRFANRPPKRLIFDRQ
                     LREAKRLLLFSDNAVNNIAWQLGFKDPAYFARFFNRLVGCSPSAYRAKKVPVT"
     gene            895..2457
                     /gene="hpaB"
                     /locus_tag="OO544_003472"
     CDS             895..2457
                     /gene="hpaB"
                     /locus_tag="OO544_003472"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
                     /protein_id="HCR6768990.1"
                     /translation="MKPEDFRASTQRPFTGEEYLKSLQDGREIYIYGERVKDVTTHPA
                     FRNAAASVAQLYDALHKPEMQDSLCWNTDTGSGGYTHKFFRVAKSADDLRQQRDAIAE
                     WSRLSYGWMGRTPDYKAAFGCALGANPGFYGQFEQNARNWYTRIQETGLYFNHAIVNP
                     PIDRHLPTDKVKDVYIKLEKETDAGIIVSGAKVVATNSALTHYNMIGFGSAQVMGENP
                     DFALMFVAPMDANGVKLISRASYEMVAGATGSPYDYPLSSRFDENDAILVMDNVLIPW
                     ENVLIYRDFDRCRRWTMEGGFARMYPLQACVRLAVKLDFITALLKKSLECTGTLEFRG
                     VQADLGEVVAWRNTFWALSDSMCSEATPWVNGAYLPDHATLQTYRVLAPMAYAKIKNI
                     IERNVTSGLIYLPSSARDLNNPQIDQYLAKYVRGSNGMDHVQRIKILKLMWDAIGSEF
                     GGRHELYEINYSGSQDEIRLQCLRQAQSSGNMDKMMAMVDRCLSEYDQNGWTVPHLHN
                     NDDINMLDKLLK"
     gene            2475..2987
                     /locus_tag="OO544_003473"
     CDS             2475..2987
                     /locus_tag="OO544_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HCR6768991.1"
                     /translation="MQLDEQRLRFRDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            3380..5530
                     /gene="btsT"
                     /locus_tag="OO544_003474"
     CDS             3380..5530
                     /gene="btsT"
                     /locus_tag="OO544_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HCR6768992.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGHLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            5580..5783
                     /locus_tag="OO544_003475"
     CDS             5580..5783
                     /locus_tag="OO544_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HCR6768993.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            5794..6750
                     /gene="yjiA"
                     /locus_tag="OO544_003476"
     CDS             5794..6750
                     /gene="yjiA"
                     /locus_tag="OO544_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HCR6768994.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYEIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEDEIRAAFAGLKK"
     gene            complement(6828..>7716)
                     /locus_tag="OO544_003477"
     CDS             complement(6828..>7716)
                     /locus_tag="OO544_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HCR6768995.1"
                     /translation="RVTEIDRLLEQKPRLTADQAWDVIRQTSRQDLNLRLFLPTLQAA
                     TSGLTQSDPRRQLVDTLTRWDGINLLNDDGKTWQQPGSAILNVWLTSMLKRTVVAAVP
                     MPFDKWYSASGYETTQDGPTGSLNISVGAKILYEAVQGDKSPIPQAVDLFAGKPQQEV
                     VLAALEDTWETLSKRYGNNVSNWKTPAMALTFRANNFFGVPQAAAEETRHQAEYQNRG
                     TENDMIVFSPTTSDRPVLAWDVVAPGQSGFIAPDGTVDQHYEDQLKMYENFGRKSLWL
                     TKQDVEAHKESQEVLHVQR"
BASE COUNT         1784 a         2102 c         2021 g         1809 t
ORIGIN      
        1 ctcatgcgtt tattacggag tctgacgccg acggtcatgt gttgacggta cgggaagatc
       61 tgatctggcc cctgctggaa gttctttatc caggcactcg ggaaaccttc ggcctgccgg
      121 ggatttgcct gtcactggca gataaacccg acgaactggc ggcgctggaa cactattggc
      181 aactgataga gcgggaatcg gtggaacaac tgcctggacg ggaacacacc ctgacgctac
      241 tggcacaggc agtgttcacc ctactgctgc gtaatgcaaa actcgacgac catgccgcca
      301 gcgggatgcg cggagaatta aaactgttcc agcgttttaa tatgctgatt gaaagccatt
      361 ttcatcagca ctggacagta ccggattacg ctaacgaact gcatatcacc gaatcacgcc
      421 tcacggacat ctgccgccgc tttgccaacc gtccgccaaa acggttgatt tttgacaggc
      481 agctgcgaga agccaagcgg ctgctgctgt tttctgataa cgccgtgaac aatattgcct
      541 ggcaactcgg ttttaaggat ccagcttatt ttgcgcgctt ttttaatcgc ttagtcggtt
      601 gctcgcccag tgcttatcgt gccaaaaaag tacctgtgac gtgaaaatcc cttagcctta
      661 acgggaaacc aggcaccacc tgctattccc ctttctccgc ctggagcaag gggatttagt
      721 aacgtcaaaa atcgctaaaa gcgaccgcga tcacaaaacc gtacccgacc tgaaaagtac
      781 cagccattgt cccaaaagtg tcttcccgta atcgcccctg caagcttcaa ttgaataaca
      841 aaaaaaacac acaaaattaa caatcgtatt ccgattaata ctgtagaggt cgacatgaaa
      901 ccagaagatt tccgcgccag tacccaacgt ccgttcaccg gggaagagta tctgaaaagc
      961 ctgcaggatg gtcgcgagat ctatatctat ggcgagcgag tgaaagacgt cactactcat
     1021 ccggcatttc gtaatgcggc agcgtctgtt gcccaactgt acgacgcgct gcacaaaccg
     1081 gagatgcagg actctctgtg ctggaacacc gacaccggca gcggcggcta tacccataaa
     1141 ttcttccgcg tggcgaaaag tgccgacgac ctgcgccagc aacgtgacgc catcgctgag
     1201 tggtcacgcc tgagctatgg ctggatgggc cgtaccccag actacaaagc tgctttcggt
     1261 tgcgcactgg gcgcgaatcc gggcttttac ggtcagttcg agcagaacgc ccgtaactgg
     1321 tacacccgta ttcaggaaac tggcctctac tttaaccacg cgattgttaa cccaccgatc
     1381 gatcgtcatt tgccgaccga taaagtgaaa gacgtttaca tcaagctgga aaaagagact
     1441 gacgccggga ttatcgttag cggtgcgaaa gtggttgcca ccaactcggc gctgactcac
     1501 tacaacatga ttggcttcgg ctcggcacaa gtaatgggcg aaaacccgga cttcgcactg
     1561 atgttcgttg cgccaatgga tgccaatggc gtcaaattaa tctcccgcgc ctcttatgag
     1621 atggtcgcgg gtgctaccgg ctcgccgtac gactacccgc tctccagccg cttcgatgag
     1681 aacgatgcga ttctggtgat ggataacgtg ctgatcccat gggaaaacgt actgatctac
     1741 cgcgatttcg atcgctgccg tcgctggaca atggaaggcg gttttgcccg tatgtatccg
     1801 ctgcaagcct gtgtgcgcct ggcagtgaaa ctcgacttca ttacagcact gctgaaaaaa
     1861 tcactcgaat gtaccggcac cctggagttc cgtggtgtac aggccgatct cggtgaagtg
     1921 gtggcgtggc gcaacacctt ctgggcattg agtgactcga tgtgttctga agcgacgccg
     1981 tgggtcaacg gggcttattt accggatcat gccacactgc aaacctatcg cgtactggca
     2041 ccaatggcct acgcgaagat caaaaacatt atcgaacgca acgttaccag tggcctgatc
     2101 tatctccctt ccagtgcccg tgacctgaac aatccgcaga tcgaccagta tctggcgaag
     2161 tatgtgcgcg gttcgaatgg tatggatcac gtccagcgca tcaagatcct caaactgatg
     2221 tgggacgcca ttggcagcga gtttggtggt cgccacgaac tgtatgaaat caactactcc
     2281 ggtagccagg atgagattcg cctacagtgt ctgcgccagg cacaaagctc cggcaatatg
     2341 gacaagatga tggcgatggt tgatcgctgc ctgtcggaat acgaccagaa cggctggact
     2401 gtgccgcact tgcacaacaa cgacgatatc aacatgctgg ataagctgct gaaataacgc
     2461 agcaggaggt taagatgcaa ttagatgaac agcgcctgcg ctttcgtgac gcgatggcca
     2521 gcctgtcggc agcggtaaat attatcacca ccgagggcga cgccggacaa tgcgggatta
     2581 cggcaacggc cgtctgctcg gtcacggata caccaccatc gctgatggtg tgcattaacg
     2641 ccaacagtgc gatgaacccg gtttttcagg gcaacggtaa gttgtgcgtc aacgtcctca
     2701 accatgagca ggaactgatg gcacgccact tcgcgggcat gacaggcatg gcgatggaag
     2761 agcgttttag cctctcatgc tggcaaaaag gtccgctggc gcagccggtg ctgaaaggtt
     2821 cgctggccag tcttgaaggt gagatccgcg atgtgcaggc aattggcaca catctggtgt
     2881 atctggtgga gattaaaaac atcatcctca gtgcagaagg tcacggactt atctacttta
     2941 aacgccgttt ccatccggtg atgctggaaa tggaagctgc gatttaagct ccagcccctt
     3001 cacgcattcg tcgaaggggc atttttattg tgatctcaat cacatatcca cccgatgaat
     3061 aattcttgcc caaccgctca ccgccattcc tgaccgctta cgtccctaaa ataaccactc
     3121 agttatttac cttactttac gcgcgcgtaa ctctggcaac atcactgcag gatagcggtc
     3181 aatttacctc ctcaaacgca acgcaaacct agaacggctt cggcctatga ttaacaaatt
     3241 actctatgaa ttttggtttg caggaaggca gcaagtgagt gaatccccgg gagcttacaa
     3301 cagtaagtga caggggtgaa cgaacgcaac tgccgcacct gtaagccaaa agacgacgag
     3361 taaaatgcca ggttttacta tggatacgaa aaaactattc aagcacatac cctgggtgat
     3421 tctcggaatc atcggtgcat tctgcctcgc ggtagttgca ttacgtcggg gggagcacgt
     3481 cagcgccctg tggatcgtgg tcgcctctgt gtcggtgtat ctggtggcgt atcgctacta
     3541 cagtctgtac atcgcccaga aggtgatgaa actcgacccc acgcgcgcga cgcctgcggt
     3601 tattaacaac gacggtctga actacgttcc gaccaaccgt tacgtgttgt ttggtcacca
     3661 cttcgccgct atcgccggtg ctggtccgct ggtcggtccg gttctcgccg cacagatggg
     3721 ccacctgcct ggcacgctgt ggctgctggc gggggtagta ctggccggtg cggttcagga
     3781 ctttatggtg ctgtttatct cctctcgccg taacggcgca tctctcggtg agatgataaa
     3841 agaagagatg ggaccagtac cggggactat cgcgctgttt ggctgtttct taatcatgat
     3901 catcatcctt gccgtcctgg cgctgattgt ggttaaagcc ctggccgaaa gtccgtgggg
     3961 tgtcttcacc gtttgctcaa ccgtaccgat tgcgctgttt atgggtatct acatgcgctt
     4021 tatccgtccg gggcgtgtgg gtgaagtctc tgtcattggt atcgtgctgc tggttgcctc
     4081 tatctacttc ggtggcgtga ttgctcacga tccgtactgg ggtccggcac tgacctttaa
     4141 agacaccacc attaccttcg cgctgattgg ctatgcgttt gtttccgcat tgctgccggt
     4201 gtggctgatc ctcgcaccgc gcgactatct ggcaaccttc ctgaaaatcg gcgttatcgt
     4261 cggcctggcg ctgggtatcg tggtgctgaa cccggaactg aaaatgcctg cgatgaccca
     4321 gtacattgac ggtactggcc cgctgtggaa aggtgctctg ttcccgttct tgttcattac
     4381 catcgcctgt ggtgcggtat ctggcttcca cgcactgatc tcttccggta cgacgccgaa
     4441 actgctggct aatgaaaccg acgcgcgttt cattggctac ggcgcaatgc tgatggagtc
     4501 cttcgtggcg attatggcgc tggttgctgc gtccatcatc gaaccgggtc tttacttcgc
     4561 gatgaacacc ccgcctgctg gccttggcat caccatgcct aacctgcatg aaatgggtgg
     4621 cgagaacgcg ccgatcatca tggcgcagct gaaagacgtt accgcacacg cggcagcgac
     4681 cgtcagctct tggggcttcg tgatttcgcc agagcagatc ctgcaaaccg cgaaagacat
     4741 tggtgagcct tctgtcctga accgtgcagg tggtgcgcca acgctggcgg taggtatcgc
     4801 tcacgtgttc cacaaagtgc tgccgatggc tgacatgggc ttctggtatc acttcggtat
     4861 tctgttcgaa gccctgttca tcctgaccgc gctggatgcg ggtacccgtt ctggccgttt
     4921 tatgctgcaa gacctgctgg gtaacttcat cccgttcctg aaaaaaaccg actctctggt
     4981 tgccggtatc atcggtactg cgggctgtgt gggtctgtgg ggctacctgc tgtatcaggg
     5041 cgtggtcgat ccgctgggcg gcgttaagag cctgtggccg ctgttcggta tctccaacca
     5101 gatgctggca gccgtagcgc tggtactggg caccgttgtg ctgattaaga tgaagcgcac
     5161 ccaatacatc tgggtaactg ttgttccggc tgtatggctg cttatctgca ccacctgggc
     5221 gctgggtctg aaactgttca gcaccaaccc gcagatggaa ggcttcttct acatggcaag
     5281 ccagtacaaa gagaagattg ctaacggtac tgacctgacg gcgcagcaga ttgccaacat
     5341 gaaccacatc gttgtgaaca actacaccaa cgcaggcctg agtattctgt tcctgattgt
     5401 ggtgtacagc atcatcttct acggtttcaa aacctggctt gcggtgcgta acagcgacaa
     5461 acgtactgac aaagaaactc cgtacgttcc aatcccggaa ggcggcgtga agatctcttc
     5521 gcaccactaa ccgtgtttag ccccgcttcg gcggggcttt gttcttatca gagtgaacta
     5581 tgtttggtaa cttaggacag gcaaaaaaat atctcggcca ggcggcgaag atgttgattg
     5641 gcattccaga ctacgacaac tacgtcgagc atatgaagac caaccatccc gacaagccgt
     5701 acatgagcta tgaagaattc ttccgcgaac gccagaacgc gcgctacggc ggcgatggta
     5761 aaggcggtat gcgctgttgt taatggagag agcatgaacc cgattgcagt taccctactc
     5821 accggttttt taggcgcggg caaaaccacc ctgctgcgcc atattcttaa cgaacaacac
     5881 ggctacgaga ttgccgtgat tgaaaacgaa ttcggcgaag tctccgttga tgatcaactg
     5941 attggcgacc gcgccacgca gatcaaaacg ctgaccaacg gctgcatctg ctgttcgcgc
     6001 tccaacgagc tggaagacgc actactcgac ctgctggata atctcgacaa gggcaatatt
     6061 cagttcgacc gtctggtcat tgaatgcacc ggcatggccg atcccggccc gattattcag
     6121 accttcttct cccatgagat tttatgccag cgctacctgc tggacggggt gattgcgctg
     6181 gtggatgcgg tacatgccga tgagcagatg aaccagttca ccatcgccca gtcccaggtt
     6241 ggctacgccg accgtattct gctgacgaaa accgacgtcg caggcgaagc agagaaactg
     6301 cgtgaacgcc tggcgcgcat caacgcccgc gcaccggtct acaccgttac ccacggcgat
     6361 atcgacctgg gccttctgtt caacaccaac ggttttatgc tggaagaaaa cgtcgtcagc
     6421 accaaaccgc gtttccactt tatcgcggac aaacaaaacg atatttcgtc gattgtggtg
     6481 gaactggatt acccggtaga tatcagcgaa gtttcccggg tgatggaaaa cctgctgctg
     6541 gaatcggcgg ataaactgct gcgttacaaa gggatgctgt ggattgacgg cgaacctaac
     6601 cgcctgctgt tccagggcgt ccagcgcctc tacagcgccg actgggaccg cccgtggggc
     6661 gatgaaaaac cgcatagcac gatggtgttt atcgggattc agttgccgga ggatgagatt
     6721 cgggcggcgt ttgcgggact caaaaagtaa ccagccctcc aacatcacaa tacctgagac
     6781 tttatcaccc tcatattcct ccagaatatg agggcattcg ggcttaatta tctctgaacg
     6841 tgcaacactt cctgcgactc cttatgcgcc tccacatcct gcttcgttaa ccagagcgac
     6901 ttacgaccaa aattttcgta cattttcagc tgatcttcat agtgctgatc aactgttcca
     6961 tcgggagcaa taaacccact ctgaccgggt gcgaccacat cccaggcaag caccggacga
     7021 tcgcttgtcg ttggtgagaa aacaatcata tcgttttctg ttccacggtt ttgatactcc
     7081 gcctgatgac gcgtttcttc cgctgcggcc tgcggtacac caaagaaatt atttgcacgg
     7141 aacgttaagg ccattgcagg tgttttccag ttactcacat tattgccata gcgtttggaa
     7201 agagtctccc aggtatcttc cagcgcagcc aacacaacct cctgctgtgg tttcccagca
     7261 aacagatcaa ccgcctgtgg gattggtgat ttgtctccct gcaccgcctc atacaaaatt
     7321 tttgctccaa cacttatatt cagcgaacca gttgggccgt cctgggttgt ttcgtagcca
     7381 ctggcgctgt accacttatc aaatggcata ggtacggcag ccactacggt acgcttcaac
     7441 atactggtca gccaaacgtt caggatggca gagcctggct gctgccaggt tttaccatca
     7501 tcattaagca aattgatgcc atcccaacgt gttaatgtat ccaccaactg acggcgcgga
     7561 tcgctctgtg tcaaaccaga tgtcgctgct tgcagagtag gtaaaaaaag cctcaggtta
     7621 agatcctgac gactggtttg gcgaataaca tcccatgcct gatcagcagt taagcgtggc
     7681 ttttgctcaa gcagtcggtc gatctccgta acgcga
//
LOCUS       DAJTZL010000178         7708 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.178, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000178 DAJTZL010000000
VERSION     DAJTZL010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7708
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.178"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..304)
                     /locus_tag="OO544_003478"
     CDS             complement(<1..304)
                     /locus_tag="OO544_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6768996.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTVWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVCPPLWR"
     gene            complement(1125..1337)
                     /locus_tag="OO544_003479"
     CDS             complement(1125..1337)
                     /locus_tag="OO544_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncH family protein"
                     /protein_id="HCR6768997.1"
                     /translation="MLCFLIYITLPFSQLVYFISSEKKLTIHIVQMFHLLSQVFYNLK
                     KFLMMDMLGVGDAININANKNIRQVC"
     gene            complement(1413..2030)
                     /locus_tag="OO544_003480"
     CDS             complement(1413..2030)
                     /locus_tag="OO544_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HCR6768998.1"
                     /translation="MIKVYGVPGWGSTISELMLTLADIPYQFVDVSGFDHEGTSRELL
                     KTLNPLCQIPTLALENDEIMTETAAIALMVLDRRPDLAPPVGRAERQQFQRLLVWLVA
                     NVYPTFTFADYPERWAPDAPEQLKKNVIEYRKSLYIWLNSQLTAEPYAFGEQLTLVDC
                     YLCTMRTWGSGHEWFQDNATNISAIADAVCQLPKLQEVLKRNEII"
     gene            2297..3796
                     /gene="ansP"
                     /locus_tag="OO544_003481"
     CDS             2297..3796
                     /gene="ansP"
                     /locus_tag="OO544_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HCR6768999.1"
                     /translation="MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKEGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQEIVFKPETAS"
     gene            complement(3911..4972)
                     /locus_tag="OO544_003482"
     CDS             complement(3911..4972)
                     /locus_tag="OO544_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HCR6769000.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGVTINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKESVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTARQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            5214..7316
                     /locus_tag="OO544_003483"
     CDS             5214..7316
                     /locus_tag="OO544_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HCR6769001.1"
                     /translation="MKIFSVRQTVLPALLALSPVVFAADEQTMIVSAAPQVVSELDTP
                     AAVSVVDGEEMRLATPRINLSESLTDVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVTTQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPGDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDEASKLSLIFNSVDIKADDPGGLTKAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSAQDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGGVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNSGMPEYGQKGELRRDERN
                     LMWNIDPYLQTQWQLSEKLSLDAGVRYSSVWFDSNDHYVTPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNIYLAAGRGFETPTINELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVNA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYVPEDGWYAGTEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNLTVDLFGRVDNLFDKEYVGSVIVNESNGRYYE
                     PSPGRNYGVGMNIAWRFE"
     gene            complement(7352..>7708)
                     /gene="mcbR"
                     /locus_tag="OO544_003484"
     CDS             complement(7352..>7708)
                     /gene="mcbR"
                     /locus_tag="OO544_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HCR6769002.1"
                     /translation="LTPQDLTELQELLEKLQQAQEKGDMEQIINVNRLFRLAIYHRSN
                     MPILCEMIEQLWVRMGPGLHYLYEAINPAELREHIENYHLLLAALKAKDKEGCRHCLA
                     EIMQQNIAILYQQYNR"
BASE COUNT         1964 a         1801 c         1856 g         2087 t
ORIGIN      
        1 tgcgccacag cggtgggcat accttcatgc tgtacagcat ctggtttttg tcgttctccc
       61 ccagttcaaa ccaggtcacc acacctttca cccggcgctg tacgtcatcg ccctgccata
      121 ccgtcaggta ggccattttg tccagcacct gcgcaaattc aagggagaga aactgctggc
      181 tgaccagaga gagatcgagg gaaaagagcg aagagagtga ctggttcaaa tgaaaggaaa
      241 ccaccgcaaa agcatccggt ggcaggccgt ccacttccag tgtgaaacgt aatccggttg
      301 acatagcaac ctccgtgaaa atgaataata atccgcacat attgcatgta caacatgtcg
      361 ggcggatgag ataagcgagt cgacatctgg cgttaccggg gaatatcaga aaaatgtcat
      421 ggttatgtga tgatttagtc tgggatttta cttcagagaa acgggtgtct gtttagtcaa
      481 caaaataatc acaaacgcaa cggatgggtt ctcgacgtta ttcacgacag gctcgtacat
      541 tacagcgagt aataaaaaac ctgatatgca aagtcaaacc aatattcata atgtcttttt
      601 attcacaaca ataaataatt caataacaat cattgaaatg aatgatggtt tgtataagga
      661 atatacgcta ccgcccaccc tgataacatg atctgcacat tgccatctta acgtcgttaa
      721 acactggctt aatagtggat atttcaaatt aggaatttat taaattcacc tgatgaatat
      781 aataagcaaa tagcatagaa aataatggca actccccccg agaggaccat ttccaaagca
      841 ctgcaccacc acctggtgcc cggatgatct tgcttcagtg gtaagagtac ctgattcatt
      901 gttcccaaaa tgatgttttc cccggccccg ccaataatgt gcagctctca cgtgataatc
      961 tattgattag gttatattaa gaatcaatat ttaaaataca tccattttct tgaataaatc
     1021 aacatattta tttatcaatt gctgttttag ctctgactca tcaagctctc catatttatc
     1081 ctatagataa ttgcgaataa atacagagaa cgccatttat ccttttagca tacctgccgg
     1141 atatttttat ttgcattaat attaattgca tctccaactc cgagcatatc catcattaaa
     1201 aactttttga gattatagaa tacctgtgat aacaagtgaa acatctgcac aatgtgaata
     1261 gttagtttct tttcagagga aatgaaatag acaagctgac tgaaggggag agtgatataa
     1321 atcaaaaaac ataacatgaa tttaattaat tacgcccatc attgtggata aagttactga
     1381 ataataaata acataatcct ctggtatccc actcaaataa tttcattcct ttttaatact
     1441 tcttgtaatt tcggtaactg acatacagca tcagcaatcg cactgatatt cgtggcatta
     1501 tcctgaaacc attcatgccc agacccccat gtgcgcatag tgcaaagata acagtccacc
     1561 agcgttagtt gctcaccaaa cgcataaggt tcagcggtca attgtgaatt cagccagata
     1621 taaagcgatt tacgatattc aataacattc ttctttaact gttcaggtgc gtcaggagcc
     1681 caacgctcag ggtaatcggc gaaagtgaat gttggataga cattagcaac cagccagacc
     1741 aatagccgtt gaaactgctg acgttcggcg cgcccaaccg gtggcgcaag gtccgggcga
     1801 cgatcgagaa ccatcaatgc aatcgccgct gtctccgtca taatctcgtc attttccagc
     1861 gccagtgtcg gaatctgaca caacgggttc agggttttca ataactcgcg tgaagttccc
     1921 tcgtggtcaa aaccactgac atcaacgaac tgataaggaa tatcagccag tgttagcatc
     1981 agctcactga ttgtagagcc ccatccgggt acgccataca ccttaatcat atgcccctca
     2041 caatctttta aagattaagt gtaggacagt acacaggtta accagcaaaa gtatttgtca
     2101 ttttttcgtc agttgtatga atccccttca ggattgtaag gggatgtttt gcatcgtata
     2161 tcgataacgg gtaggatgct gcgccgatag ttttataccc ataaagaata atggtgataa
     2221 ctatcatcgc caggatgaat aaacattgtt catggcaact tatatgactt tttcattaaa
     2281 gcaatcaggg agagcaatga gtaaacacga caccgacact tcagatcaac acgccgcgaa
     2341 acgccgctgg cttaatgccc acgaagaggg gtatcacaaa gcgatgggca atcgccaggt
     2401 acagatgatc gccattggcg gcgcgattgg caccggcttg tttttaggtg caggagcccg
     2461 actgcaaatg gcgggtccag cactggcact ggtttattta atttgtggct tgttttcgtt
     2521 ttttattctg cgtgcattgg gtgagctggt gctacaccgc ccttccagtg gcagttttgt
     2581 ctcttatgcc cgtgagtttt tgggtgagaa agccgcttat gttgctggct ggatgtactt
     2641 catcaactgg gcgatgacgg ggattgttga tattaccgcc gtcgctctgt atatgcatta
     2701 ctggggtgcg tttggcggcg tgccgcagtg ggtctttgcg ctcgctgcac ttaccatcgt
     2761 tggcaccatg aatatgatcg gtgtgaaatg gtttgcggag atggagttct ggtttgcgct
     2821 tattaaagtg ctcgccattg tgaccttttt ggtcgtgggt acagtgttcc tcggtagtgg
     2881 tcagccgctg gatggcaaca ccactggctt tcatttaatc accgataatg gcggcttctt
     2941 cccccacggt ttgctgcctg cgctggtgtt gattcagggc gtagtgtttg cttttgcctc
     3001 cattgaaatg gtgggtacag ctgccggaga atgtaaagat ccgcagacca tggtgcctaa
     3061 agccattaac agtgtgattt ggcgtattgg cctgttttac gtcggctccg tggtgttgct
     3121 ggttatgtta ttgccgtgga gcgcgtatca ggcggggcaa agtccgttcg tgacgttttt
     3181 ctctaaactg ggtgtgccat atatcggcag cattatgaac attgtggtgc tgaccgctgc
     3241 cctctccagc ctgaactcag gtctgtactg caccgggcgt attctgcgct cgatggcgat
     3301 gggcggttcc gcaccgagtt ttatggcgaa aatgagtcgt cagcatgtgc cgtatgccgg
     3361 gattctggcg acactggttg tgtatgtcgt cggcgtattc ctcaactatc tggtgccgtc
     3421 gcgcgtattt gagattgtgt tgaacttcgc gtcgctggga atcatcgctt catgggcgtt
     3481 tatcatcgtg tgccagatgc gcctgcgtaa agcgattaaa gaaggcaaag cagcggatgt
     3541 cagttttaaa ctgcctggcg cgcccttcac ttcctggctg acattactgt ttttactgag
     3601 tgtccttgtg ctgatggcgt tcgattaccc gaacgggact tatactatcg cggcgctgcc
     3661 gattatcggt attctgctgg ttataggctg gtttggtgtg cgcaaacgcg ttgctgaaat
     3721 tcacagcact gcgccagtcg tcgaggaaga tgaagaaaaa caggaaattg tgtttaagcc
     3781 tgaaacggcg agctaattgc aaaattgccc ggctacgtgc cgggcaaatt tagattactg
     3841 acgaaggtgt gccgcatgcg gcgtgataaa agtcaggcaa gtctctccgt tttcaccctc
     3901 gccatatcta ttacagcgca atacgaatca catcgtctgg ctgggtagct tcctgctgtt
     3961 tagtggattt ttgtttcaca ctgacataca gcgttttgcc atcggcagac agcgccaggc
     4021 tgtttggatg agtcggcgta tcgaacgttt tcactacttt atagcttttc gcgtcaatca
     4081 cactgacttt acctgcctga cgatgcgtta cgtaggcttc attacgcgct gggttaaaca
     4141 gcacagccag tgattccggt gctgcaacct tcgccagaat attgccatta cgggtatcga
     4201 ccactaacac ttctgcggct ttagaatcgg tgataaatgc acgctgccta gcggtatcaa
     4261 ggctaatgtt gataaagaag tgctctttgc cgtcatccag cagcttttta cggctgagga
     4321 ttttattgtc ggcggtgtcg atggtaatca attcgccgtc agcgttagtg gtgtaaagac
     4381 gtttgccttt gctatcaagc gccagaccgg tactcatttt accggtgttc tggatggcgg
     4441 ttttcagttt gatattctcg ccatcaacga cccaaatcac gctctcttta ccaataccac
     4501 tgatgtaaac ggtgttcgtg gcatcgtcag ctaccagctc acgcggttgc agcgggcgca
     4561 cctcttccgt acgcttacga tcatccagca ccagacggcc tttcacctcg cccgttttgg
     4621 catctatcgc cgtgaccgcg ctgtttacag tgttaccaaa ccacagtgtc tgagtcgtgt
     4681 tattgatggt gacaccaaac ggcttgagat cgttatggat cgcctgcgtc acttccagag
     4741 tgaccggatc aagacgataa accacgccgc ctttatccag tttgcggctt tgcgaagtgg
     4801 cgagccacag cgcgttttct tgctggctat aagccatttc gtaggcacct ttacctaccg
     4861 ctttacgcag catttcttct gcggcctgcg tactgaatga tgaagcaaca agcaatgaac
     4921 ctaacagtaa tgaaccacgc aggcgcgatg aaaacagatg acgtaaatgc atgacgactc
     4981 cctttgatga accgatattt ttgctgcttc acaatgcttc gctaagcgta atcatcgtta
     5041 cgctcttgtt ataaatggga atggtaatca ttattttctc aaatgtggag catttcttgc
     5101 cgttacgact acaattttct caattactta acgtatcgtg ccgtttcgtt ttccatgaat
     5161 ttacaatttt attaccataa cggcgttttc ccattcttaa cctggctttt ttcatgaaga
     5221 ttttttccgt ccgacagacc gttttgcccg cactacttgc cctttccccc gttgtttttg
     5281 ccgctgatga acagaccatg attgtcagtg ccgcaccgca ggtggtttca gaactggata
     5341 ccccagcagc agtaagcgtg gtggatggcg aggagatgcg cctggcaaca ccgcgcatta
     5401 acttgtccga atcactgacc gacgtgcctg gtttgcaggt acaaaaccgg cagaactatg
     5461 cgcaagattt acagctgtcg attcgcggat ttggctcccg ctccacttac ggtattcgcg
     5521 gtattcgcct gtatgtggac ggtattcccg ccaccatgcc cgacgggcaa gggcaaacat
     5581 ccaacatcga tttaagcagt gtgcaaaatg tggaagtgct gcgtggcccc ttctctgccc
     5641 tgtatggcaa cgcgtctggt ggggtaatga atgtcaccac ccagaccgga caacagccac
     5701 caaccattga agccagtagt tactacggca gttttggcag ctggcgctat gggctgaaag
     5761 caacgggcgc aacgggagac ggcacacagc ctggcgatgt cgattacacc gtctcaacca
     5821 cgcgttttac gacccacggc tatcgtgacc atagtggcgc acagaaaaat ttagccaatg
     5881 ccaaactggg cgtacgcatt gatgaagcca gcaaattaag tctgattttc aatagtgtgg
     5941 atatcaaagc agatgacccg ggtgggctaa ccaaagcaga atggaaggct aatccacaac
     6001 aagcgcctcg tgcagaacag tacgacacgc gaaaaaccat caagcaaact caggctgggt
     6061 tgcgctatga gcgtagcctg agcgcgcaag atgatatgag tgtgatgatg tatgccggag
     6121 agcgagaaac gacccagtac cagtcaatac ccatggcacc acaacttaac ccgtcacatg
     6181 cgggcggcgt gattaccctg caacgccatt accaggggat agacagccgc tggacacacc
     6241 gtggtgaact gggcgttccg gtcacgttca ctaccggcct gaactacgaa aacatgagtg
     6301 aaaaccgcaa gggctacaat aacttccgcc tgaatagcgg catgccggag tacgggcaaa
     6361 aaggtgagtt gcgtcgcgac gaacgcaatc tgatgtggaa catcgatccc tatttacaga
     6421 cgcagtggca gctgagcgaa aaactgtcgc tggatgctgg cgtgcgctac agctccgtgt
     6481 ggtttgattc caacgaccat tacgttactc cgggtaacgg cgatgacagc ggtgatgcca
     6541 gttatcataa atggctacct gccggttcgt taaaatatgc aatgaccgat gcctggaata
     6601 tctatctggc agccgggcga ggttttgaaa cgccgacgat taatgagctg tcttatcgtg
     6661 ctgatgggca aagcggtatg aactttggtt taaaaccatc caccaacgat acaattgaga
     6721 tcggcagtaa aacgcgtatt ggtgatgggc tgcttagtct cgcattgttt cagaccgaca
     6781 ctgatgatga aattgttgtc gatagcagta gcggtgggcg tacgacttac aaaaatgccg
     6841 gaaagacccg tcgtcaaggc gctgaactgg catgggatca acgtttcgca ggagattttc
     6901 gcgtaaacgc gtcctggacc tggcttgatg cgacctatcg cagcaatgtt tgcaatgaac
     6961 aggattgtaa cggtaatcgg atgccaggga tcgcccgtaa tatgggcttt gcgtcgatag
     7021 gttatgtacc ggaagatggt tggtatgcag gcacggaagc gcgttatatg ggcgatatta
     7081 tggcagatga tgaaaatacg gcaaaagcgc cgtcttatac tctcgtcggc ttattcaccg
     7141 ggtataaata caattaccac aatttaactg tggatttatt tggtcgtgtc gataatttat
     7201 tcgataaaga atacgttggt tctgtcattg tcaatgagtc aaacgggcga tattacgaac
     7261 cttcgcccgg acgaaattat ggtgtcggca tgaatattgc gtggagattt gagtaattac
     7321 cttgatgccc ggtatttgcc gggcatttac tttaacgatt gtattgctgg tataaaatag
     7381 caatattttg ttgcataatt tcagcaagac aatgtctgca tccctctttg tcttttgctt
     7441 ttaacgcagc gagtaacaga tgatagtttt ctatatgctc ccgtaattcc gctggattaa
     7501 tcgcttcata gagataatgt aaaccaggcc ccatcctgac ccacagttgc tcaatcatct
     7561 cacacaggat gggcatattt gagcgatgat aaatcgctaa gcgaaatagc ctgtttacat
     7621 taatgatttg ttccatgtcg cccttttctt gcgcctgctg taatttctcg agcaattcct
     7681 ggagttccgt caggtcttgc ggggtgag
//
LOCUS       DAJTZL010000179         7623 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.179, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000179 DAJTZL010000000
VERSION     DAJTZL010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7623
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.179"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..96
                     /locus_tag="OO544_003485"
     CDS             <1..96
                     /locus_tag="OO544_003485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR6769003.1"
                     /translation="PAASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
     gene            complement(152..487)
                     /locus_tag="OO544_003486"
     CDS             complement(152..487)
                     /locus_tag="OO544_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HCR6769004.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(491..835)
                     /locus_tag="OO544_003487"
     CDS             complement(491..835)
                     /locus_tag="OO544_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HCR6769005.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            complement(837..1010)
                     /locus_tag="OO544_003488"
     CDS             complement(837..1010)
                     /locus_tag="OO544_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6769006.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            1111..1296
                     /locus_tag="OO544_003489"
     CDS             1111..1296
                     /locus_tag="OO544_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6769007.1"
                     /translation="MSRRYSSATITLVILFCRRPHCRKISVRQYYLKRVIFYPTTLQR
                     TAFKKCLFLMRDIIISS"
     gene            1257..1376
                     /locus_tag="OO544_003490"
     CDS             1257..1376
                     /locus_tag="OO544_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HCR6769008.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(1855..2121)
                     /gene="minE"
                     /locus_tag="OO544_003491"
     CDS             complement(1855..2121)
                     /gene="minE"
                     /locus_tag="OO544_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HCR6769009.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(2125..2937)
                     /gene="minD"
                     /locus_tag="OO544_003492"
     CDS             complement(2125..2937)
                     /gene="minD"
                     /locus_tag="OO544_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HCR6769010.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            complement(2961..3656)
                     /gene="minC"
                     /locus_tag="OO544_003493"
     CDS             complement(2961..3656)
                     /gene="minC"
                     /locus_tag="OO544_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HCR6769011.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            4176..4544
                     /locus_tag="OO544_003494"
     CDS             4176..4544
                     /locus_tag="OO544_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HCR6769012.1"
                     /translation="MNMMRIIYIGLSGVGMMFSSMASGHDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSLQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVD
                     GKRSGKINQTTTKMLFMCRE"
     gene            complement(4652..5053)
                     /locus_tag="OO544_003495"
     CDS             complement(4652..5053)
                     /locus_tag="OO544_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HCR6769013.1"
                     /translation="MKIKSISKAVLLLALLTSTSFAAGKNVNVEFSKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            5295..5588
                     /locus_tag="OO544_003496"
     CDS             5295..5588
                     /locus_tag="OO544_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HCR6769014.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            5660..6319
                     /locus_tag="OO544_003497"
     CDS             5660..6319
                     /locus_tag="OO544_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HCR6769015.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFISAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            6411..6857
                     /locus_tag="OO544_003498"
     CDS             6411..6857
                     /locus_tag="OO544_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HCR6769016.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMICAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(7064..>7623)
                     /gene="hlyE"
                     /locus_tag="OO544_003499"
     CDS             complement(7064..>7623)
                     /gene="hlyE"
                     /locus_tag="OO544_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hemolysin HlyE"
                     /protein_id="HCR6769017.1"
                     /translation="VLDDGITKLNEAQKSLLVSSQSFNNASGKLLALDSQLTNDFSEK
                     SSYFQSQVDKIRKEAYAGAAAGVVAGPFGLIISYSIAAGVVEGKLIPELKNKLKSVQS
                     FFTTLSNTVKQANKDIDAAKLKLTTEIAAIGEIKTETETTRFYVDYDDLMLSLLKEAA
                     KKMINTCNEYQKKHGKKTLFEVPEV"
BASE COUNT         1957 a         1714 c         1772 g         2180 t
ORIGIN      
        1 ccggcggcca gtgtctgggg taatgtgggc gtgcagctgg gtgataatgg ctacaatgac
       61 accgcagtga tggtgggcct gaaatataag ttctgatccc cgttagctaa aataccgcgt
      121 cgaattcatc gacgcggtaa catattaaat attatttttt gccgatagca cgcatggtgt
      181 catcaattgc cgtgatcaac agcatttgcg ggtctttagc gcaaacctga ttcagttttt
      241 ctaccacttt ggcgctcagt tccggagatt gctcaatttt taaatcacgg ctggcaacgc
      301 tggcattacc catcaccgca acaatttctg caacctgtgc gctgtcagtt tttgccattt
      361 cgttggcttc tgcgcaagta atataggttt ctgacggcaa accgttttta atattgtagt
      421 cctgcgccca ggtcattggt gcgaaaacaa acaggcccgc cagtaaagca aattttttca
      481 tcatcattcc ttatttcatt ttacccagaa ttgcaccacc cgtaccgcca atcacggcac
      541 ctttaatcgc cccttcgagg ccattgccgg tcagaacgcc agtgacagca ccaacggcgg
      601 cacccacttt tgcaccttta cgtgcatttt taccgtcgcg gcctttttct gttactgcac
      661 caacaccagc gccaacagct gcacctttca gtacgccatt aacaccattg ccagtaagta
      721 atccaacgcc tgcgcctagc aatgcacctt tcgtggtgcg gttcatatcc gccatcgctg
      781 gcgtggagca gaacaatgct gagataagcc cgaaggcaag tatttttttc ttcaacttag
      841 atgtccggta ttaagtaagt tgcacacaca ataatttcgt cttcaattaa gatctgctta
      901 actaaagaac gctcgctatt attcagataa ttcaaaatga gcgtggctgt gatgatagga
      961 attatgtttt ttacgtgaat gagaataatc ttaaatgagg aataactcat tgattggcaa
     1021 tatttttatt caagaagtat cattgactgt taacgcaatg ttgtaaaggt aagataatct
     1081 gatttatcaa tattattgtg tgatttttat gtgagcagaa gatattcatc agcaacgatt
     1141 acattagtca ttttattttg ccgacggcct cattgtcgaa agataagcgt acgacagtat
     1201 tatctgaaaa gagtgatttt ttatccaact acacttcagc gcactgcgtt taaaaaatgc
     1261 ctctttctta tgcgggatat catcatttca tcatgatgtc tttggtgagc ggtgaacaca
     1321 atacacctgc gctgtctctt caggatgaat cccctggtct ggtgcctgcg ggctgatgtt
     1381 gcagcagagc ttaggtcact tagacgctac tatcatttat ccaatggcat ggaatcgaaa
     1441 tcagtcgata cccgcagtat atatcgtgaa ctggtcgttc tgaccatcaa cgattaaact
     1501 gcgcttcggc gcagttttcg tttacagggg gctaaaaggg aaaattctgg ggcaaaaaaa
     1561 gcccgccagt tacggcggga aacctcatcc tatgggagaa caatgaataa tgaaattgcg
     1621 gggttatcat cccccagtgt atccacacta acaataaggt tatttactca gccaggcata
     1681 aacattatgt tctgtgcgtg ggaacagcct taaggtgtaa agggggaggt ggaaatagca
     1741 atgaggagta tcagcaagaa tactcgccgc tttaccacaa cgtggatgag agggatgaaa
     1801 aactcaaggc agaaatgact ctgccttgaa gataaatgcg tttttacagc gggcttattt
     1861 cagctcttct gcttccggta aggtcacgtt cagctcaaga atagaaatat cgccatcttt
     1921 ttgctcaagc tgtacggtta ccatctcagg atcaatttgt acgtatttac aaatgacctc
     1981 aagaatatct ttacgcaact gcggcagata atgcggttct gcatcgctgc gacggcgttc
     2041 agcaacaata atctgcagcc gttcttttgc aatgttggct gtgtttttct tccgcgagag
     2101 aaagaaatcg agtaatgcca taacttatcc tccgaacaag cgtttgagga agcctttctt
     2161 ctcttcttca atgaagcgga aaggacgttc ttctcccaac agacgttcta cggtatctgc
     2221 gtaggcttta cccgcatcgg cgttaatgtc gagaatgacc ggttcaccct ggttagaggc
     2281 gcgtaatact gattgatcct ctgggatcac gccgacgagt ttgatgcgca ggatctccag
     2341 cacatcttcc atgctcagca tgtcacctct gcttacgcgg cctgggttat agcgcgttaa
     2401 cagcaggtgc tctttaatag gctcttcgcc attttctgcg cggcgtgatt tcgacgccag
     2461 aatgcctaaa atacggtcag agtcgcgtac tgaggagact tccgggttgg tggtaataat
     2521 ggcttcgtct gcaaaataga gtgccattaa cgcaccggtt tcaatccctg ccggggagtc
     2581 acaaacgata aattcaaaat ccatcgcttt cagatcatca agaactttgg cgaccccttc
     2641 acgggtgagg gcatctttat cgcgtgtttg cgatgccgga agaatataga gattttcagt
     2701 acgcttatct ttaattaacg cctgatttag cgttgcatcg ccctgaatga cgttgacgaa
     2761 atcgtaaacg acccggcgtt cgcaacccat aatcaggtcg agattacgca ggccgatatc
     2821 aaaatctatc acgacagttt tctttccctt ctgggccaaa ccagtggcga tggccgcgct
     2881 ggaggttgtc ttaccaaccc cccctttgcc cgaagtaaca acaataatgc gtgccataga
     2941 aattccttgt taaaaaggga tcaatttaac ggttgaacgg tcaaagcgtt ttcgactaac
     3001 tgcagtcgcg ccgctttgcc ataaaattct gctgggattt gatcactcag ccagtattca
     3061 cctgcgatgg acaccaattc cgccatcagg ttcgtacaaa atatttgcgt ttcccggtca
     3121 ccacttgccc ctgccagcgc acgaccgcgc atcatgccat agacatgaat gttcccatcg
     3181 gcaatcaatt cggccccagc gctaacgtgg cttgtaacaa tcagatcaca ttgtggagca
     3241 taaatacgct gaccggaacg caccggggta tctattaaac gcgtttttgt gaccggcgtg
     3301 gtattttgcg ctggagcctg tggtgcggga gctggacgtg gcgctttttc ctttccttcc
     3361 gtcaggatag gcagccccat tttttcaatt tcggctttaa gttgcgcgtc tttgcagcca
     3421 cttacgccaa taacccgcaa accggttgcc gaaaccgcct tatgcatcgc tgaccagttt
     3481 accgggtctt ccagtgcact gacgttgagt acaacggggg catgttttaa aaatgcgggg
     3541 gcctgagcga ttttgtcttc cagcgcctga tggataacct taggttctgc ctcatgcaga
     3601 tgaaccacag ataaagtgaa gctactgcct ttaagctcga ttggcgtgtt tgacatcctg
     3661 gccttactca attagctatt aatcatcgcc agcgcgcgat gatgttccga agactataag
     3721 gcatgttata gtctggatta tattgaggca agtcaccctc ccatttattc agagtaaaag
     3781 tctattctgt gataaatggc gctgattcat agcttaaaaa atacccttgt caatcaaccc
     3841 attgccgccg tacttttgat tcttcttatt tacgcttcct ttttccgcac cctaatcgtg
     3901 acagcatctc cagacctgtt taaatatcca gcattgaggc aagtgtattt tttcaccacg
     3961 ccaggctttc ttaactttcc attttttgca gcatcattaa agaacagata cccgttatct
     4021 taatatccgc cacagaattg ctctatcata gaaaccgcag aagacgtctg atacttctgt
     4081 caggtcatcg taactcgtcc aggtcagcgg ttttgattac ggattgatga cgaaatgagc
     4141 ataagtgttg tacggttgga ttatcaggat gtgctatgaa tatgatgcgt attatttata
     4201 tcggattgtc aggtgtgggg atgatgttct catcaatggc gtctggacat gacgctggcg
     4261 gacttcaatc tccggcgtgc ggcgttgttt gcgacccgta tatttgtgtg aactcagatg
     4321 gcatttcccc agagttaaca aggaaatatc tcggcgaaaa agccgctgaa aacttacaat
     4381 cattacaagg ctacgatccc agcgaattta cattcgctaa cggtgtattt tgcgatgtta
     4441 aagaaaaatt atgtcgtgat gatcgttatt ttggtgtaga tggtaagcgg agtggaaaaa
     4501 tcaatcaaac caccacaaag atgttattta tgtgtcgtga gtgatgcctt gtttacatta
     4561 tgttctctgc tattcgtcat taacctcttg agcgacagag aggagtgggg cgttaacgtc
     4621 tatgatgggg atagtccccg tcggttgaca tttacttaat ttgaatatcg acgttatatt
     4681 ttttagtttt gtttttacgg gcgtcgtttc gagtttgcag cactcgcagc tcatatttgc
     4741 cagaagcagg gagtgagtat tgtccgtgac tatcgagttc tggggaatat ctggataaat
     4801 caacagaatc atcgattcct ggcccgaaga gataagtatc agcgccttca ttagagatac
     4861 tcacatgcac tttctgacct tttttggcat agaaggtata tgtatcgtaa tcgtatccct
     4921 ttatttcgcc ggagtactga gcgctgctat ggcctttgct gaactcaaca ttgacatttt
     4981 tacccgcagc aaagctcgtg gaggttagca gtgcaagcag caatacagcc ttactgatgc
     5041 tcttgatttt cattggtagt ctcctgttca gaatgagtac gatgaactgt tataatataa
     5101 caatccctta cgggagaatt ccagtggctt ttctgcgttc ggtaaaatcc cggcgcggca
     5161 ttttttgccg gggggattac ttataaaggg gcatggactc aacaaaccgc agaaatctac
     5221 agctggttgg cttgtggcgc gggtttcatt gatgttagct tatgccaaaa cctggcatct
     5281 taaagagtaa aagtatgttt tgtgtgattt atcgaagcag caagcgtgac cagacctatt
     5341 tatatgtcga aaaaaaagac gatttttcgc gtgttcctga ggaactgatg aaaggttttg
     5401 gtcagcctca gttagcgatg attctgccgt tggatgggcg gaagaaactg gtcaatgctg
     5461 atattgaaaa ggttaaactg gcattaaccg agcaaggtta ctatttgcag ttaccgccac
     5521 cacctgaaga tttgctgaag caacatcttt ccgtcatggg gcagaaaaca gacgacacta
     5581 acaaataacc gatatccggc ggtggcatta tctttgtcgg cgcgggtttt catatccacg
     5641 ataaggtgag gggaacgtta tgtatcaaca tcacaactgg caaggtgcgc tgctggatta
     5701 tccggtgagt aaagtagtct gtgttggcag taactatgcc aaacatatta aagagatggg
     5761 cagcgcagtg cccgaagagc cagtgctgtt tattaaacca gaaacggcac tgtgcgatct
     5821 gcggcagcca ttggcgatcc catccgattt cggttcagtt catcatgaag tcgaactggc
     5881 ggtgttgatt ggcgcgacgc tgcgtcaggc aacggaagag catgtccgca aagccattgc
     5941 cggttacggc gtggcgcttg atctgacatt gcgtgatgtt cagggaaaaa tgaagaaagc
     6001 cgggcagccg tgggaaaagg ctaaagcgtt tgataactct tgtccgcttt ccgggtttat
     6061 ctccgcggcg gaatttaccg gcgatccaca aaatacaacg ctgggcctga gcgtaaacgg
     6121 cgaacaacgc cagcaaggta cgactgcgga catgatccat aaaatcgttc cgttgatcgc
     6181 ttatatgagc aagtttttta ccctcaaggc cggtgacgtt gtgttgacag gcacgcctga
     6241 tggcgtcggc ccgttgcaaa gcggtgatga gctgacagtc actttcgatg ggcattcttt
     6301 gacaactcgc gttttgtaat actttttgcc gcctgaaagc ggcggcaaaa cttgcatcgc
     6361 tgtgccagac tggttataag gtgcgtttta acgtaatggc ggaacacctg atgagcgatg
     6421 tacctttctg gcaaagtaaa accctggacg aaatgagcga tgcggaatgg gagtcgttgt
     6481 gtgatggttg cggtcagtgt tgcctgcata aactgatgga tgaagacacc gacgaaatct
     6541 acttcactaa cgtcgcctgt cgccagctca atattaaaac ttgtcaatgt cggaactacg
     6601 aacgtcgttt cgagtttgaa cccgactgca ttaaattaac ccgtgaaaat ctgccaacat
     6661 tcgaatggct gccaatgatc tgcgcttatc gtttgctggc ggaaggtaaa gatttacctg
     6721 cgtggcatcc gctacttact ggttcgaaag cggcaatgca tggtgaacgt atctctgtgc
     6781 gtcatatcgc agtgaaagaa tcagaagtga ttgactggca ggatcatatc ttaaataaac
     6841 ctgactgggc gcagtgataa atttaataga tgttgatggg ggctttctta atatacgtct
     6901 ggatgctcgc catttcgtag ccctgttttt gaaagcgctc tgtcataatg agagttaccc
     6961 gataccttat tatcaacagg taactctcaa tctttaaaaa acgtctgtgt ttttaaatgt
     7021 gataaacctt ataccttgag tacatggaga gaataatcgc tgatcagact tcaggtacct
     7081 caaagagtgt ctttttaccg tgttttttct gatactcatt acaggtgtta atcatttttt
     7141 tggccgcttc ttttagcaaa gaaagcatta aatcatcata atcaacgtag aatctggtgg
     7201 tttcagtttc cgtttttatc tctccgatgg cggctatttc ggtggttaat ttcaatttgg
     7261 cggcatcgat atctttattc gcttgtttaa ccgtgttaga cagggtggta aagaaactct
     7321 gcacagattt taacttgttc ttcaattctg ggatcagttt cccttcaact acgcccgcag
     7381 caatagaata ggaaatgatt aaaccaaatg gaccggcgac gacaccggct gcggcaccgg
     7441 catacgcttc cttcctgatt ttatctacct gtgactggaa atagctgctt ttttctgaaa
     7501 aatcattggt taactggcta tctaacgcca gcagtttccc ggaagcgttg ttgaaacttt
     7561 gtgagcttac cagcagggat ttttgcgctt cattcagctt cgtgatgccg tcatccagta
     7621 cct
//
LOCUS       DAJTZL010000180         7491 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.180, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000180 DAJTZL010000000
VERSION     DAJTZL010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7491
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.180"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(16..522)
                     /gene="mug"
                     /locus_tag="OO544_003500"
     CDS             complement(16..522)
                     /gene="mug"
                     /locus_tag="OO544_003500"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HCR6769018.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
     gene            647..722
                     /locus_tag="OO544_003501"
     tRNA            647..722
                     /locus_tag="OO544_003501"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:680..682,aa:Ile,seq:cat)
     gene            complement(776..1540)
                     /gene="nfeF"
                     /locus_tag="OO544_003502"
     CDS             complement(776..1540)
                     /gene="nfeF"
                     /locus_tag="OO544_003502"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase"
                     /protein_id="HCR6769019.1"
                     /translation="MNNSPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELAID
                     FFIHDGGVASGWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK"
     gene            1828..2451
                     /gene="nfeR"
                     /locus_tag="OO544_003503"
                     /gene_synonym="yqjI"
     CDS             1828..2451
                     /gene="nfeR"
                     /locus_tag="OO544_003503"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HCR6769020.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHSRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDITDAQIKKIIAVIDHAAFDITQLD"
     gene            complement(2558..4078)
                     /gene="aer"
                     /locus_tag="OO544_003504"
     CDS             complement(2558..4078)
                     /gene="aer"
                     /locus_tag="OO544_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HCR6769021.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGFT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTSKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLAAMLWFVAAPVVTYFLCVLVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            4496..5875
                     /gene="ygjG"
                     /locus_tag="OO544_003505"
     CDS             4496..5875
                     /gene="ygjG"
                     /locus_tag="OO544_003505"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HCR6769022.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGSLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMQLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQADQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            complement(5917..6249)
                     /locus_tag="OO544_003506"
     CDS             complement(5917..6249)
                     /locus_tag="OO544_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HCR6769023.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRVV"
     gene            6468..7451
                     /gene="ebgR"
                     /locus_tag="OO544_003507"
     CDS             6468..7451
                     /gene="ebgR"
                     /locus_tag="OO544_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HCR6769024.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLFSVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
BASE COUNT         1825 a         2029 c         1868 g         1769 t
ORIGIN      
        1 tttcggtggc gttttttatc gcccacgcac taccagcgcc tggtccagct cgcgatacgc
       61 ttcaaccagt ttctctagtg aaacgcgact taaaccgctg ggatttggca gcacccaaat
      121 ctgcgtcgaa ccaatggtga gcgtttgttt cccccactgt gcaccgcgct ggctgaatcc
      181 ctgttcatat gcttgtttgc ccagaatcgc caacgcctgc ggctgataat cttcaatttt
      241 ttcaatcagc ttacgcccac ctgcgtgcag ctcctgcttt gaaacttcat tggcttgcac
      301 cgttggacgg tctaccagtt tggtgacgcc acaacgataa tccagcagat gctgtgcctc
      361 ctgcggcttc aactgacggt cggtaaaccc ggcctgatat atcaccttcc agaagcgatt
      421 tgctggatga gcaaagggaa aaccagtccc ggcggatgaa agcccagggt tgataccgca
      481 aaatacgacc cgtaaccctg gagccaaaat atcctcaacc atatttactc ctttctaaca
      541 tcaccaggaa aagtataacg gattgaaaat acattgttta taaaaacagc aggcgcacgg
      601 taatggctgg attgcgacac ggagttactt tataatccgc taccatggcc ccttagctca
      661 gtggttagag caggcgactc ataatcgctt ggtcgctggt tcaagtccag caggggccac
      721 cagatatagc aaaggctgac gagaaatcgt cagccttttt ctttttatat atcagttact
      781 ttgcgtgcca gtaagccgct gcacgtaccc gctgtgggtc atactgttcc gcttcaaagc
      841 ggcggcttaa attcttaacg actttacctt cgccggttat ccagatgaag taatcatcgg
      901 cagggatttg catctgcgcc agacgcgcat ctaccgcctg ctcatcatgt gccagccatt
      961 cgatattaaa accatcaagg tgcgcgagat aatcctgaca ggcgttatcc cgcacgctaa
     1021 ccagcgcact aacttgcggt ttaacggcaa gtttgctcaa cgtttccaag cggcggcgca
     1081 gtgcgggcat tccggattca tcgcagacat acagctgata cgcgtaatct tccggcacca
     1141 ccagcgaacc gcgcggacct gcaaccgtaa gtttatcgcc cggttgcgcc tgcatcgccc
     1201 agccgctggc gaccccaccg tcgtgaataa agaaatcgat cgccagttca tggcgtagtt
     1261 cgtcatacag cggcgtatag tcacgcgacg gtgggcgtgg tccttccggc cagacgatgc
     1321 cctcttccgt taccgttggc ggcacaaagt gagcgtcagg ttgaggaaag aagagtttgc
     1381 tgtgatcgtc aaagccgcgc gatgtaaaac cgtccagcgc ctcgccgccg aggacaatgc
     1441 gctgaaaacc ggcgctgatg cgctcaacgc gtaacacagt cagttcacgg aagcgcagat
     1501 cattgcgaac gcgctgcggg tagcgggggg agttattcat tgttatcgcc ttcgtgatgg
     1561 taatcagata tatctaaata attttcgtaa atgataatga ttgttaatca tgataaatgc
     1621 aagcgatttg tagaactgat atgtctatag tctgataaga cgaaccgcct cttctcaggc
     1681 atcattactc aacgccggat gcggcgtgaa cgccttatcc ggcctacgtg tgagatgagt
     1741 ccacatttaa aaatcatttt tattcttgca agagtgctca tatcagatat aaattagata
     1801 tatctaatca agcaaaagga ggctgatatg agccatcatc acgaagggtg ttgtaaacat
     1861 gaaggccagc cacgccatga gggctgctgc aaaggtgaga agtcagaaca cgagcactgc
     1921 ggacacggtc accagcatga acacggtcaa tgctgcggtg gtcgccacag tcgcggcggc
     1981 ggtcgtcggc aacgtttctt tggtcacggt gaattgcgtc tggtgattct ggatattctc
     2041 tcgcgcgatg acagccacgg ttacgaattg attaaagcga ttgagaatct aacccagggg
     2101 aattacaccc caagcccggg cgtcatctac ccaacgctgg attttctgca ggagcagtcg
     2161 ctgattacca tccgcgaaga ggaaggtggt aagaagcaga ttgcgctgac cgaacaaggc
     2221 gcgcagtggc tggaagaaaa ccgcgaacag gtggagatga ttgaagaacg catcaaagcg
     2281 cgttgcgttg gtgctgcgct gcgccagaac ccgcaaatga agcgggcgct ggataatttt
     2341 aaagcggtgc tggatttacg cgtcaaccag agcgatatca ctgatgcaca aataaaaaag
     2401 atcattgcgg tgatcgacca cgccgctttt gatattaccc aactggatta atcaccgatt
     2461 gccggatgcg gcgtacccta cacaccccgc aggcctgata agatgcgcca gcatcgcatc
     2521 aggcattgtg ctccaaccgc cggatccggc ataccgatta atgcagcacc gtcaccgcgt
     2581 cttccagtcg gctggcgcgg tgtttcacca tcgccgacac ctgcgcactc tcttccacca
     2641 gctcagcatt tttctgggtg atcaggttaa gctcatccac tgcacgggtc aggctggaaa
     2701 gcccatcggc ctgttccagc gttgaatggc taatctgggc gatcaactgg gtgacgtttt
     2761 tcacctgtgc cacaatatct tccatcgtcc gtccggcggc gtgtacctgc tgcgaaccgg
     2821 attgcacctt atcagcactg gcatcaatca gcttgcgaat atcgttggca gcattagcgc
     2881 tgcggctggc taaatgacgc acttcccctg ccaccactgc aaaacctttg ccctgttcgc
     2941 cggcacgcgc cgcttccacc gccgcattca gggccagaat attggtctga aacgcaatat
     3001 cgttaatcag cgaagtaatg gtgccaatgc gctgggtact gtcggcgata tcgtccattg
     3061 tcttgatcac cgtggtcatc gcctccccac cctgcactgc cgcattactg gcggtgatag
     3121 aaagtttatc ggcagccgac gccgtggcag agttctgttt caccgacgcc gccatttggt
     3181 tcatggtcgc caccgtttgc tgaacgttat caactgtctg ctgggtatgt tcgttcagtt
     3241 catcggtgcc tttcgccagc gtctcactgc catttctgac actggacacc tggcttgaga
     3301 catcgttaat tagccaacgg cacatcaggc caagctgccc taccgcgcgt aatgtcagcc
     3361 ccagctcatc gctgcgattc aggtgctcaa cactattacg ctctccggtc gccaccttca
     3421 gtgcctgacg ggcgacattt tctatcgggc gcacaatctg ccattcaaaa caagcgcttg
     3481 ccaacaatac cactaacaca cagaggaaat acgtcaccac cggggcagca acaaaccaaa
     3541 gcatggccgc cagcaagata aacatcaggg tcatcactcc acgcgcccgc cagcgaagcg
     3601 gtaatgaagg cagtttaccc agccagcctt tacgcaccac caggccttta tgaatacgct
     3661 tactggtacg tccggcgttc agcgctttgt acagcggctc caccgccgca atctcttcat
     3721 ccgtcgcccg ggtacgaatc gacatatagc cactgatttt tccctcgcgc accatcggta
     3781 ccgcattggc ccgcacccaa taatggtcgc catttttgcg gcgatttttc acgatgccgc
     3841 tccagggctc cccttttttc agggtgaacc acatatccgc aaacgccgct tttggcatat
     3901 ccgggtgacg caccatgttg tgcggctgcc cttgtaactc ttgcaaggta aagccgctca
     3961 cctgcacaaa agtgtcatta gcatgagtga tatagctttg cagatcggta gtggacatca
     4021 gagtggtatc gtccgccagc ggggtatttt gctgggtgac atacggatga gaagacatgt
     4081 tcgcgtcctg tgcaggttat atggttgtta acttcttgtc agagtttatg tcggccccgc
     4141 tgcggttatc tttaaccgat taatttgatt tagatcgcaa tttgcgatta aagcacaaat
     4201 ctaattcttt gatttaaaat actttcactc tgttactata cgaaaacgtt aattatcttg
     4261 cccaaaaatc aggcaattat tgccctgaaa acgtgcattt gcgcagcaat catcaaatcc
     4321 atacccgaca aaaaccgtgc aaaataacaa caaatgttaa cagatagcat taaatattgc
     4381 acaaatgata accgaatttg tgtttatccc gattttcgcg atcgcagccg gagtggcgca
     4441 atccctgcaa tacttaaatc ggtatcatgt gatacgcgag cctccggagc atattttgaa
     4501 caggttacct tcgagcgcat cggctttagc gtgcagcgcc cacgccctga atctcattga
     4561 gaagcgaacg ctggatcatg aggagatgaa agcacttaac cgagaggtga ttgaatactt
     4621 caaagagcat gtcaatccgg ggtttttaga gtatcgcaaa tctgttaccg ccggcgggga
     4681 ttacggagcc gtagagtggc aagcgggaag tttaaatacg cttgtcgaca cccagggaca
     4741 ggagtttatc gactgcctgg gaggttttgg aattttcaac gtggggcacc gtaatccagt
     4801 tgtggtttcc gccgtacaga atcaacttgc gaaacaaccg ctgcacagcc aggagctgct
     4861 cgatccgtta cgggcgatgt tggcgaaaac ccttgctgcg ctaacgcccg gtaaactgaa
     4921 atacagcttc ttctgtaata gcggcaccga gtccgttgaa gcagcgctga agctggcgaa
     4981 agcttaccag tcaccgcgcg gcaagtttac ttttattgcc accagcggcg cgttccacgg
     5041 taaatcactt ggcgcgctgt cggccacggc gaaatcgacc ttccgcaaac cgtttatgca
     5101 gttactgccg ggcttccgtc atgtgccgtt tggcaatatc gaagccatgc gcacggctct
     5161 taacgagtgc aaaaaaaccg gtgatgatgt ggctgcggtg atcctcgaac cgattcaggg
     5221 tgaaggtggc gtaattctgc cgccgccggg ctatctcacc gccgtacgta agctatgcga
     5281 tgagttcggc gcactgatga tcctcgatga agtacaaacg ggcatggggc gcacgggcaa
     5341 gatgttcgcc tgcgagcatg agaacgtaca gccggatatc ctctgccttg ccaaagcgct
     5401 cggcggcggc gtgatgccga ttggcgcgac catcgccact gaagaggtgt tttcagttct
     5461 gttcgacaac ccattcctgc ataccaccac ctttggcggc aacccgctgg cctgtgcggc
     5521 ggcgctggcg accatcaatg tgttgctgga gcagaactta ccggctcagg ctgatcaaaa
     5581 aggcgatatg ttgctggacg gtttccgtca actggcgcgg gaatatcccg atctggtaca
     5641 ggaagcgcgt ggtaaaggga tgttgatggc gattgagttt gttgataacg aaatcggcta
     5701 taactttgcc agcgagatgt tccgccagcg cgtactggtg gccggaacgc tcaataacgc
     5761 caaaacgatc cgcattgaac cgccactgac actgaccatt gaacagtgtg aactggtgat
     5821 caaagcggcg cgtaaggcgc tggcggccat gcgagtaagt gtcgaagaag cgtaatatca
     5881 aatcggatgg cgatacgacg tcgccatccg atctttttat acgacgcgta cgcccgcagg
     5941 catcatccgc tccggcgtta acagcacgct ttcactgccg tcatcggttt ccgcacacaa
     6001 cagcatgcat tccgacgttt caccacgcat tttcgctttt tgcagattgc ataagaccac
     6061 caccgttttt cccatcagct cctcttcgct gtagtacggc accaggctgg tcacggtttg
     6121 cagtgttttt tcccccacat caacctgtac gatgtacagc ttgtcggcgt tttcatggcg
     6181 tttcacttcc acaatctttc cgacgcgcat ttccagacgt gcaaaatcag cgtaagccac
     6241 ggtttccatt gcttcctccc ttcaagtaaa attttactaa actatagcaa aattttttct
     6301 caatcctgta ggctaaaaat ggagaatgca ggcgtgatca cattcgtaag ccgctgtgtt
     6361 accgttacag cgtcaaagaa acgcgcttta tttactgaaa acaggtgacc cgataagcac
     6421 ttcctctaca atgggggcgc acatcaggga aagtaaaaaa ggtaaacatg gcaacattaa
     6481 aagacatcgc aatcgaagct ggcgtatccc tggcgacagt atccagggtc ttaaatgacg
     6541 atccgacatt gaatgtgaaa gaagagacga aacatcgcat tctcgagatc gccgaaaagc
     6601 tggagtacaa gaccagtagc gcccgtaaac tacagacagg cgcagtcaac caacaccata
     6661 ttctggctat ctacagctac cagcaggagc tggagatcaa cgatccttac tatctggcga
     6721 tccgccacgg cattgaaact cagtgcgaaa agctggcgat cgaactgacc aactgttatg
     6781 aacacaacgg attaccagac attaaaaacg tcaccggtat tttaattgtc ggcaaaccca
     6841 cgcccgccct gcgcgccgct gccagcgcgt tgaccgacaa tatctgtttt atcgactttc
     6901 acgaacccgg cagcggttac gatgcggtgg atatcgatct ggcacgcatc agtaaagaaa
     6961 tcatcgactt ctatatcaac cagggcgtta atcgtattgg ttttattggc ggtgaagatg
     7021 agcctggcaa ggcggatatt cgtgaggtcg cctttgcgga atatggccga ctgaaacaag
     7081 tggtacgcga agaggatatc tggcgcggcg gtttttccag ttcgtcgggt tatgaactgg
     7141 caaaacaaat gctggcgcgg gaagactatc cgaaggcact gtttgttgct tccgattcca
     7201 ttgctatcgg cgtactgcgg gcaattcatg aacgtggcct gaacatccca caggatattt
     7261 cgcttttcag cgttaacgat atccccaccg cgcgatttac ctttccgccg ctctccaccg
     7321 tgcgcatcca ttccgagatg atgggaagtc agggcgttaa cctggtgtat gaaaaagccc
     7381 gcgatggtcg cgcgctgccg ctgctagtct tcgttcccag caaattaaaa ctgcgcggca
     7441 cgacccgtta aatcccctta cacactgtcc ggcaatcatt tttgccggac a
//
LOCUS       DAJTZL010000181         7453 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.181, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000181 DAJTZL010000000
VERSION     DAJTZL010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7453
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.181"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..834
                     /locus_tag="OO544_003508"
     CDS             <1..834
                     /locus_tag="OO544_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCR6769025.1"
                     /translation="EDLMPWRWPRIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGI
                     SSGAAFGVVLMLFLVPGNAFGWLLPAGSIGAAVTLLIIMIAAGRGGFSPHRMLLAGMA
                     LSTAFTMLLMMLQASGDPRMAQVLTWISGSTYNATDAQVWRTGIVMVILLAITPLYRR
                     WLTILPLGGDTARAVGMALTPTRIALLLLAACLTATATMTIGPLSFVGLMAPHIARMM
                     GFRRTMPHIVISALVGGLLLVFADWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQS
                     R"
     gene            complement(992..2272)
                     /gene="hemL"
                     /locus_tag="OO544_003509"
     CDS             complement(992..2272)
                     /gene="hemL"
                     /locus_tag="OO544_003509"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HCR6769026.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIIEPVAGNMNCVPPLPD
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVEPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            2497..3918
                     /gene="clcA"
                     /locus_tag="OO544_003510"
     CDS             2497..3918
                     /gene="clcA"
                     /locus_tag="OO544_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HCR6769027.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            3942..4007
                     /locus_tag="OO544_003511"
     CDS             3942..4007
                     /locus_tag="OO544_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6769028.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            4000..4344
                     /gene="erpA"
                     /locus_tag="OO544_003512"
     CDS             4000..4344
                     /gene="erpA"
                     /locus_tag="OO544_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HCR6769029.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(4391..5014)
                     /locus_tag="OO544_003513"
     CDS             complement(4391..5014)
                     /locus_tag="OO544_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HCR6769030.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEVGPLIAVCMVVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(5052..5852)
                     /gene="btuF"
                     /locus_tag="OO544_003514"
     CDS             complement(5052..5852)
                     /gene="btuF"
                     /locus_tag="OO544_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HCR6769031.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(5845..6543)
                     /gene="mtnN"
                     /locus_tag="OO544_003515"
     CDS             complement(5845..6543)
                     /gene="mtnN"
                     /locus_tag="OO544_003515"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HCR6769032.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            6627..>7453
                     /gene="dgt"
                     /locus_tag="OO544_003516"
     CDS             6627..>7453
                     /gene="dgt"
                     /locus_tag="OO544_003516"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HCR6769033.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEQLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVA"
BASE COUNT         1683 a         1924 c         2043 g         1803 t
ORIGIN      
        1 gaggatttga tgccctggcg ctggccgcga attatggcgg cgctgtttgc gggcgtcatg
       61 ctggcggtgg cgggctgtat tattcagcgg ctgaccggaa acccgatggc aagcccggaa
      121 gtgctgggga ttagctctgg cgcggcgttt ggcgtggtgt taatgctgtt tctggtgccg
      181 ggtaatgcct ttggctggct gttacccgcg ggcagtatcg gggcggcggt gacgctgttg
      241 atcattatga tcgccgccgg ccgcggtgga ttttccccac accgtatgtt actggcgggg
      301 atggcgttaa gtaccgcatt caccatgctt ttgatgatgt tgcaggcgag cggtgacccg
      361 cgaatggcgc aagtgctgac ctggatttcc ggttcgacct acaacgcgac cgatgcgcag
      421 gtctggcgta ccggaattgt gatggtgatt ttgctggcga ttaccccgct gtaccgccgc
      481 tggctgacca ttttaccgct gggtggtgat accgcacgag ccgtaggaat ggcgctgacg
      541 ccgacgcgaa ttgcgctgct gctgttagcg gcttgcctga cggcgaccgc gacgatgact
      601 attggaccgt tgagttttgt tggtttaatg gcaccgcata ttgcgcggat gatgggcttt
      661 cgacggacga tgccacacat cgtaatttcg gcgctggtgg gtggtttact gctggtattc
      721 gctgactggt gtgggcggat ggtgctgttt ccattccaga tcccggcggg gctgctgtcg
      781 acctttatcg gcgcgccata ttttatctat ttgctgagaa agcagagccg ttaatttttc
      841 catataacgg ccctgtacgc ctggacggat aaagcgttta cgcgacaacg gatgcccgat
      901 gcgacgctgg cgcgtcttat cgggcctaca aacggccccg aatcgtaggt cggataaggc
      961 gttcacgccg catccgacaa accatgctgg atcacaactt cgcaaacacc cgacgtgcag
     1021 catcgatggt gttattgata tcttccatgc tgtgcgccac ggacataaag cccgcttcaa
     1081 acgctgacgg tgccaggtaa acaccttcgt ccagcatcat atggaagaaa cgcttaaagc
     1141 gttccacgtc acaggccatc acatcctgat agcacgtcac ggactcggcg tcggtaaaga
     1201 aaataccgaa catgccgcca acgtggttaa cgaccagcgg aattccggct tcttctgccg
     1261 cttccagcag accttctgcc agacgtgttg tcagctcatc caacgtttcg tgaacgcccg
     1321 gttgcgcgac ttcattcaga caggcgaaac cggctgccat cgcaattggg ttaccggaaa
     1381 gcgtacccgc ctgatagacc ggacccgtcg gggccagcgc atccattaca tcacgacgac
     1441 caccgaatgc gcctaccggc attccaccgc cgatgatttt gcccaggcag gtgagatccg
     1501 gttccacacc gtaataatcc tgtgcgccag ccagtgccac gcggaagccg gtcattactt
     1561 catcgatgat cagcaatgcg ccaaattcgt cgcacagcgc acgcagacct ggcaggaagt
     1621 ctggcagcgg tggaacacag ttcatattgc ctgccaccgg ctcgatgata atacaggcaa
     1681 tctcttgcgg gtattgctca aacgcggcgc gtacagaagc cagatcgtta taagtacagg
     1741 ttaaggtatg tttggcgaaa tctgccggaa cgcccggcga gtttggctgg cctaacgtga
     1801 gtgcgccaga accggctttc accagcaggc agtcagcgtg accgtggtaa cacccttcaa
     1861 atttaatgat tttgtcgcga ccggtaaaac cacgggccag acggatggcg ctcatcgtcg
     1921 cctcggtgcc ggagttcacc atgcgcacca tatccatggt cgggaccaat tcggtcacca
     1981 gttgcgccat tttcacttcc atttcggttg gtgcaccaaa gcttaaacca cgctcggcgg
     2041 cttcaatcac ggcattgcgg attgccggat ggttatggcc cagcaccatc ggcccccagg
     2101 aaccgacata atcgatatag gctttgccat caacatcgta cagataagcg ccgtccgctt
     2161 tttcgataaa cagtggagtg ccgcccacgc cagtaaaggc gcgaacaggg gagttcacac
     2221 cgccagggat cagctcgcgc gctgcgctgt aaagattttc agacttactc atggagggtt
     2281 cctgattcgt agaaaaagtg aatggctgct attctatgtt attcataaca agttaaatac
     2341 tcgtcaaaca tcaggctgct tgtactggtg caatcctgat ttcttagagt ataaaagttt
     2401 tgtgcatttg aaacattacg ctttgcaaag gattttcatg gaacgtgcga gtaaaatgcc
     2461 gtcatcttat ttgtatgacc aataagtgat cattggatga aaactgatac tccctcttta
     2521 gaaacaccgc aggccgcgcg cctgcgacgc agacaactga ttcgccaact tcttgagcgc
     2581 gataaaaccc cgttagccat tttgtttatg gcggcagtcg tcggcacgct tgttgggctg
     2641 gcagcggttg cttttgacaa aggtgtcgcc tggttgcaga accaacgtat gggggcgctg
     2701 gtacatactg ctgataatta tccgcttctg ttaaccgtcg cttttctctg ttcggcggtg
     2761 ctggcgatgt ttggctactt tttggtgcgc aaatacgcgc cggaagcagg tggttcgggg
     2821 atcccggaaa ttgaaggggc gctggaagat caacgtcccg ttcgctggtg gcgtgtattg
     2881 ccggtgaagt tctttggcgg gctggggaca ctcggcggag gcatggtgtt ggggcgcgaa
     2941 gggccaaccg tgcagatcgg cggtaacatt ggccgtatgg tgcttgatat tttccgcctg
     3001 aaaggtgacg aagctcgcca tacgctgctg gcaaccggtg ctgctgcggg gctggctgcg
     3061 gcctttaacg cgccgctggc gggtattttg tttattatcg aagagatgcg tccgcagttt
     3121 cgctatacgt taatttcgat aaaagcggta tttattggcg tcattatgtc gaccattatg
     3181 taccggattt ttaatcatga agttgcgttg attgacgtcg gtaaactttc tgatgcgccg
     3241 cttaatacgc tgtggcttta tctgatcctc ggtattattt ttggcatttt cggccctatt
     3301 tttaataaat gggtgctggg gatgcaggat ttgctgcacc gtgtgcacgg cggcaatatt
     3361 accaaatggg tactgatggg cggtgcgatt ggcggtctgt gtggattgct ggggtttgtg
     3421 gcaccagcaa cgtcgggcgg cggttttaac ctgattccta tcgctaccgc ggggaatttc
     3481 agcatgggaa tgctggtgtt tatcttcgtc gctcgggtca ttaccacctt actctgcttc
     3541 tcttccggcg cgccgggcgg tatttttgcc ccgatgctgg cgctgggtac tgtgctggga
     3601 accgctttcg gaatggttgc cgttgagctg tttccgcaat atcaccttga ggcggggacg
     3661 tttgctattg ccggaatggg ggcattactg gcggcatcta ttcgcgcgcc gttaacgggg
     3721 atcattctgg ttctggagat gaccgataac taccagctca ttttgccaat gattattacc
     3781 ggtcttggcg caacactatt agcgcaattt accggcggga aaccgctata ctcggcgatt
     3841 cttgcgcgca cgctggcaaa acaggaagct gagcaactgg cgcgaagcaa ggccgcatca
     3901 gccagcgaga atacttgaac gaaataccag ggtattagat aatggcgatt attattgggt
     3961 tagaatttgc ccaattgccg atgtcgtttg gagcaaaata tgagtgatga cgtagcactg
     4021 ccgctggagt ttaccgacgc agcagccaac aaagttaaaa gcctgatcgc tgacgaagat
     4081 aacccgaatc tgaaattacg cgtgtatatc accggtggcg gttgcagcgg cttccagtat
     4141 ggtttcacct ttgatgatca ggtgaacgaa ggcgatatga ccatcgaaaa acagggcgtt
     4201 ggcttggtgg ttgatccgat gagcctgcaa tatctggtcg gcggttccgt tgactatacc
     4261 gaaggactgg aaggttctcg tttcatcgtg accaacccga acgcgaaaag cacctgcggt
     4321 tgcggttctt cctttagcat ctaatctgtt gttcatgtgc cggataaact atccggcgca
     4381 tcttctctct tcaacgccca ttctcatcca gcgcaaacgt cggtagctta agatgccaac
     4441 gaatagccgc cagccgaatc aatagcgtca cgaccatgcc catcatactg gctgtttcca
     4501 gtggtacgga aaatgtgtaa taagccgtag cgtggacaat accgccgata atacaggcag
     4561 ttgcgtagat ttctgtacgt aaaatcatgg ggatctcgcg ggccagtaca tcacgaatga
     4621 tcccgccgcc aacgccagta atgacgacca tacaaaccgc gattaacgga ccgacttccg
     4681 cattaaaggc tttattcacg ccaatgccga caaacaccgc cagaccaacg gcgtccagca
     4741 ccggcaacat ccattttggt aagcgtcttg gctggcgcac cagcacgatg gtcagcatgc
     4801 tggttaccat tgcaacgacc agatcggtgg gatctttcac ccaaaatacc gggccgtgat
     4861 ccagcgccat gtcgcgaatt gtcccgccgc ctacagcggt aaccacgccc agtaccagaa
     4921 caccaaaagg gtccatacgc aattttccgg ctaacaaaac gccggagatg gcaaataccg
     4981 ctgtgccgac tatatccagc caatagacga gcattgttca atccccactg agcacctaaa
     5041 aaggtcaggc gctaatctac ctgtgaaagc gcattacaga gctgttgtgc agcgaggata
     5101 atacgtgggc ttgcacgttc aaaccagtca ctcgtgagag gaataacggg aattttgagc
     5161 tgttcacccc agtattgttt gattttagga atttggtccg gtccgcctgt aatgacaatc
     5221 gcctgtggcg agcgtgctaa cacctgttcg cggctaactt gcggccaggg aacccggctg
     5281 tctttaaaga tgttttctcc gccacaaact tcgagtacct ggttttgaat cgactctttt
     5341 ccactggtaa atggcggatt aatgccgaat tgcagaaaaa cacgtttttt aggtttatca
     5401 gcatattgcg ctttcaattg cgcgtactga tccagcaggg attgcgcggc ttgttcggcc
     5461 ttatccggtt gcggactcca gggggccaat tgacgtaacg cattggcaat ttgttcaatg
     5521 cttgtcgcat cgacccacat cacttttatt cccagcgaag ccagctggtc aacctgccgc
     5581 tcggcattac ctccacgcca ggcaatcacc agatcgggtt tcagcgcgac aatgcgttcc
     5641 agattcatcc cctgccaggt ggaaacctgc tcaatctttt gcgcttgtgg aggatagtcg
     5701 gaatagctgc tgaccccaac cggcgtgatc ccggcggcaa aggcaagttc agtgttggcg
     5761 ggagaaagcg tgatgacgcg cggcgcggcg ttgagccaca gtggcgcaag aaaagacagg
     5821 gcgaccagcg ccctgaacag tgacttagcc atgtgccagt ttctgcacca gtgactcaac
     5881 catcaggctg gactgtttag cggcaacagc caggaactca tcgaagctaa gatgagactg
     5941 ttgatcggcc acgtcggaga tggcgcgtac gacaacaaac gggacgttga aattgtggca
     6001 gacatgggcg attgccgtcg cttccatctc tacagcaatg gcctgtggga agttgtggcg
     6061 gattttcgcc agaccaacag aaccgttgat gaaagcgtcg ccgctaacaa tcaggccacg
     6121 tacagcgtta agattcagtt cggcaatgca ggcctcagcg gcagcgatca gtttatcgtc
     6181 agctttaaag cctgccggac agcctggtaa ctgaccgtat tcataaccaa atgccgtgac
     6241 atccgcgtcg tgataacgcg cttcgtccga gacaacgata tcgcccactt tcagcgttgg
     6301 tgccaggcca ccggcagaac cggtgttaat aatcacatct ggcttgcagt gttccaacag
     6361 cagagtggca cccagcgccg cagcgacttt accgatgccc gatttcagaa gcgcaacctc
     6421 ggttccattc agttggccgg tatagatttc gcaaccgccg agactgatag tttgacggtt
     6481 ttcgattttg tcacgcagca gcgtaacttc ttcttccatt gcaccaatga tgccgatttt
     6541 catagattta ctcgcgataa gcccgatttg aaagcatagt ttaccatgcg cttacgggga
     6601 agcgtatttc tcacgcggga gaggacatgg cacagattga tttccgaaaa aaaataaact
     6661 ggcatcgtcg ttaccgttca ccgcagggcg ttaaaaccga acatgagatc ctgcggatct
     6721 tcgagagcga tcgcgggcgt atcatcaact ctccggcaat tcgtcgtctg caacaaaaga
     6781 cccaggtttt tccactggag cgcaatgccg ccgtgcgcac gcgtcttacc cactcgatgg
     6841 aagtccagca ggtgggacgc tacatcgcca aagaaatttt aagccgtctg aaagagctta
     6901 aattgctgga agcatacggc ctggatgaac tgaccggccc ctttgaaagc attgttgaga
     6961 tgtcatgcct gatgcacgat atcggcaatc cgccgtttgg tcattttggc gaagcagcga
     7021 taaatgactg gtttcgccaa cgtttgcacc cggaagatgc cgaaagccag cctctgactg
     7081 acgatcgctg cagcgtggcg gcactacgtt tacgggacgg ggaagaacag cttaacgagc
     7141 tgcggcgcaa gattcgtcag gacttatgtc attttgaggg gaatgcacaa ggcattcgtc
     7201 tggtgcatac attgatgcgg atgaatctca cttgggcaca ggttggcggt attttaaaat
     7261 atacccgtcc ggcgtggtgg cgtggcgaaa cgcctgagac acatcactat ttaatgaaaa
     7321 agccgggtta ttatctttct gaagaagcct atattgcccg gttgcgtaaa gaacttaatt
     7381 tggcgcttta cagtcgtttt ccattaacgt ggattatgga agctgccgac gacatctcct
     7441 attgtgtggc aga
//
LOCUS       DAJTZL010000182         7429 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.182, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000182 DAJTZL010000000
VERSION     DAJTZL010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7429
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.182"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            69..1055
                     /gene="dcyD"
                     /locus_tag="OO544_003517"
     CDS             69..1055
                     /gene="dcyD"
                     /locus_tag="OO544_003517"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HCR6769034.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGVIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCEALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEIILWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIGCISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            1070..1738
                     /gene="tcyL"
                     /locus_tag="OO544_003518"
     CDS             1070..1738
                     /gene="tcyL"
                     /locus_tag="OO544_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HCR6769035.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            1735..2487
                     /gene="yecC"
                     /locus_tag="OO544_003519"
     CDS             1735..2487
                     /gene="yecC"
                     /locus_tag="OO544_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     YecC"
                     /protein_id="HCR6769036.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKFLLQ"
     gene            2717..3439
                     /gene="sdiA"
                     /locus_tag="OO544_003520"
     CDS             2717..3439
                     /gene="sdiA"
                     /locus_tag="OO544_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HCR6769037.1"
                     /translation="MQDTDFFSWRRTMLLRFQRMEAAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPESWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFSE
                     AQPLWEAAHAHGLRRGVTQYLMLPNRALGFLSFSRCSTREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILKWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
     gene            complement(3506..3730)
                     /locus_tag="OO544_003521"
     CDS             complement(3506..3730)
                     /locus_tag="OO544_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein"
                     /protein_id="HCR6769038.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(3717..3893)
                     /locus_tag="OO544_003522"
     CDS             complement(3717..3893)
                     /locus_tag="OO544_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6769039.1"
                     /translation="MIKIFIGHYINAFNSIVDVTLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            4189..4845
                     /gene="uvrY"
                     /locus_tag="OO544_003523"
     CDS             4189..4845
                     /gene="uvrY"
                     /locus_tag="OO544_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HCR6769040.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            4842..6674
                     /gene="uvrC"
                     /locus_tag="OO544_003524"
     CDS             4842..6674
                     /gene="uvrC"
                     /locus_tag="OO544_003524"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HCR6769041.1"
                     /translation="MSDQFDAKAFLKTVTCQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            6731..7279
                     /gene="pgsA"
                     /locus_tag="OO544_003525"
     CDS             6731..7279
                     /gene="pgsA"
                     /locus_tag="OO544_003525"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HCR6769042.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
BASE COUNT         1837 a         1740 c         1958 g         1894 t
ORIGIN      
        1 ccttttttat ttacgcgttt tcagcgtgca taataagaaa atcacaacaa catacatcgg
       61 aggctgttat gccactgcat aatttaaccc gttttccacg gttggagttt atcggcgcgc
      121 caacgccgct cgaatatctg ccgcgctttt ctgattatct aggacgggaa attttcatca
      181 aacgggatga cgtcaccccc atggcaatgg gcggcaataa attacgtaag ctagaatttc
      241 tcgcggcaga tgctctgcgc gaaggtgccg atacgctgat tactgccggg gtgatccagt
      301 ctaaccatgt gcggcagact gccgcagttg cggcgaaact cggtctgcac tgtgtggcgc
      361 tgctggaaaa tcctattggc acaaccgcag aaaactattt aaccaacggc aatcgcttgt
      421 tgctggatct gttcaatacc cagattgaaa tgtgcgaagc actgaccgat cccaatgccc
      481 aactggaaga gctggcgacg cgagtcgaag cacaaggctt tcgcccgtat gtcattccgg
      541 ttggcggttc taatgctctt ggcgcgctag gttatgtgga gagtgcgctg gaaatcgcgc
      601 aacagtgtga aggggcggtt aatatttcgt cggtggtagt cgcatcgggc agtgccggaa
      661 ctcacgccgg actggctgtt gggctggaac acctgatgcc tgaaagcgaa ctgattggcg
      721 tgaccgtgtc gcgttccgtt gccgatcagt tgccgaaagt ggttaaccta caacaggcga
      781 ttgcgaaaga actggagctg accgcatcag cggaaattat actctgggat gactattttg
      841 cacctggcta cggcgtgccg aacgacgaag gcatggaagc agtgaaattg ctggcgcggc
      901 tggaaggcat tctgcttgat cctgtgtata ccggaaaagc gatggcgggg ctgattggct
      961 gtatcagtca gaaacgcttc aaagatgaag ggccgattct gtttattcat accggcggtg
     1021 cgcctgcgct gttcgcctat catccccacg tttagctccg ggaacgctca tgcaagaaag
     1081 tatacaactg gttattgatt ccttgccgtt cctgctgaaa ggggctgggt atacgctgca
     1141 actcagtatc ggcggcatgt tttttggctt actgctcggg tttattctcg cgctgatgcg
     1201 cctgtcgcca atctggcccg tgcgctggct ggcgcgtttt tatatctcta tttttcgtgg
     1261 tacaccgctc atcgcacaac tgtttatgat ctattacggc ctgccgcagt ttggtatcga
     1321 attagatccc attccgtcag caatgattgg tctgtcgctg aacactgccg cctatgccgc
     1381 cgaaacactg cgcgccgcca tttcttctat tgataaaggt cagtgggaag cagctgccag
     1441 tattggtatg acgccatggc agactatgcg tcgcgcgatt ttgccacagg cggcgcgggt
     1501 ggcgttgccg ccgctgtcga acagctttat cagcctggta aaggatacgt cgctggccgc
     1561 gacaatccag gtgccggagc tgttccgtca ggcgcagttg attacctcgc gcacgctgga
     1621 ggtgttcacc atgtacctgg cggcttcgct catctactgg atcatggcga cagtattatc
     1681 gacgttgcag aaccattttg agaatcaact taatcgccag gagagagaac caaaatgagt
     1741 gccattgaag ttaagaacct ggtgaaaaaa tttcacggtc agacggtgct gcacggtatc
     1801 gacctcgagg taaagcctgg cgaagtggtg gcaattatcg gtccgagtgg ttccggcaaa
     1861 accacgttgc tacgcagcat aaatctgctg gaacaaccgg aagcgggaac gatcaccgtt
     1921 ggcgatatca ctattgatac tgcacgttca ttaagtcagc aaaaatcgct gatccgccag
     1981 ttgcgtcagc acgtcgggtt tgtcttccag aactttaatt tgtttccgca tcgtacggtg
     2041 ctggaaaaca ttattgaagg gccggtgatc gtcaaaggcg aaccgaaaga agaggccacg
     2101 gcgcgcgctc gcgagctgct ggcgaaagtt gggctggccg gtaaagaaac cagctatcca
     2161 cgtcgtttgt ctggcggtca acagcagcgt gtagcgattg cgcgtgcgct ggcaatgcgt
     2221 cctgaggtga ttttgtttga cgagccaacg tcagcgctgg atccagagct ggtgggcgaa
     2281 gtcctgaaca caatccgcca gctggcgcag gaaaagcgca cgatggtgat tgtgacgcac
     2341 gaaatgagct ttgcccggga tgttgcggac cgggcgattt ttatggacca ggggcggata
     2401 gtcgagcagg gggcagcaaa agcgttattt gctgatcccc agcagcctcg cacccgccag
     2461 ttcctcgaga agtttctact gcaataataa gaaaaaatca gccccgacga ttcacctgtc
     2521 ggggcgggac gccatttcaa gcgtgataaa actgcttaac aaatcagcat aactcattaa
     2581 taacataaga gaatgcgatg gcttgcaaaa gtaattcatt gcctgaataa tataaattat
     2641 atataaatct tatttatgtg atagtttgaa ttatcattat aaatgatact cactctcagg
     2701 ggcgttgcgg tttactatgc aggatacgga ttttttcagc tggcgtcgca cgatgctgtt
     2761 gcgttttcag aggatggagg ccgcagaaga ggtctaccat gaaatagagc ttcaggctca
     2821 gcagctggag tacgattact attcgttatg tgttcgccac ccggtaccat tcacgcgacc
     2881 taaagtggct ttctacacca attaccctga gtcgtgggtt agttattatc aggcaaaaaa
     2941 ttttctcgca attgatcctg tgctgaaccc tgaaaacttt agtcagggcc atttaatgtg
     3001 gaatgatgac ttattcagcg aagcacagcc attatgggaa gccgcgcacg cacatggttt
     3061 acgccgcggt gtcactcagt atttaatgct gccaaatcgg gcgctgggct ttttgtcctt
     3121 ttcccgttgc agcacgcgcg aaatacccat tcttagtgat gaactgcaat taaaaatgca
     3181 gttactggtg cgcgaaagtc tgatggctct gatgcgttta aatgatgaaa tagtgatgac
     3241 gccagagatg aatttcagca agcgcgaaaa agaaattctg aaatggacgg cggaagggaa
     3301 gacatcagca gagatagcga tgattttgtc aatctctgag aatacggtca atttccatca
     3361 gaaaaacatg cagaaaaaaa tcaatgcacc aaataagacc caggtggcct gttacgcggc
     3421 cgctactggc ttaatttgat cgcttttctt tctgcgtgcc agatgcaaaa atcggctgaa
     3481 aggcacgctc tcagccggtt ttatattact gacggtaggc ttgtttaatt tgcttaaccg
     3541 ttttggaaaa tactgcagcc tgggtttcgt cttcgatcag cgcaagctgt ttttccatct
     3601 ttaacatcac gcggcccgcg tcagcttgtc ccatcgcctg tagcatcagc gtcagcatcg
     3661 ctttcaggca ggagacttca tttgccagtt cttgattatt ctcggcagta gaaaaatcag
     3721 gcgtactcat ttattttcct cgtcatgttg caatgaaaat ttgcggtgaa aaatgttaag
     3781 gcggcggagt ataccataag ctttgctaaa aatagcagtg gttgtttttt gagcgtgacg
     3841 tcaacaatgc tattaaatgc gttaatataa tgaccaataa atatttttat catgaatgtt
     3901 ttttgcccgt attgttcggg ttaattaatg ttacatattc agcgggctga ttttcatttt
     3961 tgctgaataa agtcaatttt tgtcacattt catcgccggg cttactgtga aacgatcccg
     4021 gaagccgttg gtgacgggcg tgaccataac tgtggacaat cgaatttaca aaaacgagag
     4081 aaaaatcgaa tacccaccat ttttaacgtt tcaaagttgc aataaaaacc gctaatatac
     4141 gaatgactaa ctatcagtag cgttatccct atttctggag atattccttt gatcaacgtt
     4201 ctacttgttg atgaccacga actggtgcgc gcagggatac gacgcattct ggaagatata
     4261 aagggtataa aagtcgtcgg tgaggcatcg tgcggtgaag acgccgttaa gtggtgccgg
     4321 acaaatgccg ttgacgtggt gctaatggac atgagtatgc cgggcattgg cggtcttgag
     4381 gcgacgcgta aaatcgcgcg ttccacagct gatgtcaaaa tcatcatgct taccgtccat
     4441 acagaaaacc ctttaccagc gaaagtcatg caggccggtg ctgcgggcta cctcagcaaa
     4501 ggcgcggctc cgcaggaagt cgtgagtgcg attcgttctg tctattcagg gcagcgttac
     4561 attgcttctg atatcgctca acaaatggcg ttaagccaga tcgaaccaga aaaaacagaa
     4621 agcccatttg ccagtttgtc tgaacgtgaa ttgcagatta tgctgatgat caccaagggc
     4681 cagaaggtca atgagatctc agaacagctc aatctcagtc cgaaaacggt gaacagctac
     4741 cgctatcgta tgttcagtaa actaaacatt catggcgatg ttgagctgac tcacctggca
     4801 attcgccatg gtctgtgtaa tgcggagaca ttatcaagtc agtgagtgat cagtttgacg
     4861 caaaagcgtt tttaaaaacc gtaacctgcc agccaggcgt ttatcgcatg tacgatgctg
     4921 gtggtacggt tatctatgtc ggcaaagcga aagacctgaa aaaacggctt tccagctatt
     4981 tccgcagcaa cctcgcttcg cgcaaaaccg aagcgctggt cgcccagatc cagcaaattg
     5041 atgtaacggt tacgcataca gaaaccgaag cgctgttgct ggaacacaac tacatcaaac
     5101 tctatcagcc gcgttacaac gttttgctac gcgatgataa atcttatcct tttatcttcc
     5161 tgagtggcga tacccacccg cgtctggcga tgcatcgtgg tgcgaagcat gccaaaggtg
     5221 aatatttcgg cccgttcccg aatggctatg ccgtacgtga aacactggcg ctactgcaaa
     5281 agattttccc cattcgccag tgcgaaaata gtgtttatcg caatcgctcg cgtccgtgtc
     5341 tgcaatatca gatagggcgc tgtctgggac cgtgcgttga aggactggtg agtgaagaag
     5401 aatacgctca gcaggttgag tatgtgcgcc tgtttttgtc tggcaaagat gatcaggtgc
     5461 ttacgcaact cattagtcgt atggaaactg ccagccagaa tctggagttt gaagaagctg
     5521 cacgtattcg cgaccaaatt caggcggtgc gacgcgtcac cgaaaaacaa ttcgtttcca
     5581 ataccggcga cgacctcgac gttattggtg tggcgttcga tgcgggcatg gcttgtgtcc
     5641 acgtattgtt cattcgtcag ggcaaagtgc tcggcagccg cagctatttc ccgaaagtgc
     5701 ctggcggtac ggaactgagc gaggtggtag aaaccttcgt aggccagttc tatttacaag
     5761 gcagccagat gcgcacctta ccgggtgaga tcctgctcga ttttaatctt agcgataaaa
     5821 cgctgctcgc cgattccctt tcagaactgg cgggacgcaa gattaatgtt caaaccaaac
     5881 ctcgcggcga tagggcgcgt tatctgaaac tcgcgcgcac caatgcggcg acggccttaa
     5941 ccagcaaact ttcgcagcaa tctaccgttc accagcgact gaccgcgctt gccagcgtgt
     6001 tgaaattgcc ggaagtgaag cggatggagt gctttgacat cagccatacc atgggcgaac
     6061 aaaccgtcgc ttcctgtgtg gtgtttgatg ctaacggccc gctgcgtgcg gagtatcggc
     6121 gctataacat tacaggcatc acgccgggcg atgattatgc ggcgatgaat caggtgctgc
     6181 gtcggcgtta tggtaaagcc attgacgaca gtaagatccc ggatgtgatc cttatcgacg
     6241 gcggcaaagg ccagcttgcg caggcgaaaa atgtcttcgc cgaactggat gtctcatggg
     6301 ataaaaatca tccgctgcta cttggcgttg ccaaaggagc agatcgtaag gctgggctgg
     6361 aaacgctgtt ctttgagccg gaaggtgagg gattcagttt gccgccagat tctcccgcgc
     6421 tgcatgttat ccagcatatt cgcgatgaat cacatgatca cgcgattggc gggcaccgta
     6481 aaaaacgggc gaaggtcaaa aataccagtt ccctggaaac cattgaaggc gtcgggccaa
     6541 aacgtcggca aatgttgttg aaatatatgg gcggtttgca aggtttacgt aacgccagcg
     6601 tcgaggaaat tgcaaaagtg ccgggtattt cgcaaggtct ggcagaaaag atcttctggt
     6661 cgttgaaaca ttgatgtctc tgtagcaaca taggggtaat cttactgaca acagatagtt
     6721 acccgtcatt atgcaattta atatccctac gttgcttaca ctgttccgtg tcatccttat
     6781 cccattcttt gtattggtct tttatctgcc tgtcacctgg tcgccgtttg ccgccgcgct
     6841 cattttctgc gtcgcggcgg tgactgactg gttcgatggt tttctggcac gccgctggaa
     6901 ccagagtacc cggtttggtg ctttccttga ccctgtagca gataaagttc tcgtggctat
     6961 cgccatggtg ctggtaaccg agcattacca cagctggtgg gtgaccttac cggcagcaac
     7021 gatgatcgcc cgtgaaatta ttatttctgc gctacgcgaa tggatggcgg agttgggtaa
     7081 acgcagtagc gtggctgtct cctggattgg gaaagtgaaa accactgccc agatggtggc
     7141 gttggcatgg ctgctgtggc gtccgaacat ttgggttgag tacgccggta ttgcactttt
     7201 ctttgtggct gcggtactga ctctgtggtc aatgttgcaa tatttgagcg ctgcgcgtgc
     7261 agatttgctt gatcagtgat cgtttcggcg taattttcag caaacgatca aaagtggtga
     7321 aaaatatcgt tgactcatcg cgccaggtaa gtagaatgca acgcatcgaa cggcggcact
     7381 gattgccaga cgataataaa atcaagtgat taactgattg cttgatgaa
//
LOCUS       DAJTZL010000183         7429 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.183, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000183 DAJTZL010000000
VERSION     DAJTZL010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7429
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.183"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..99)
                     /locus_tag="OO544_003526"
     CDS             complement(<1..99)
                     /locus_tag="OO544_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001433403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HCR6769043.1"
                     /translation="MTLTRREFIKHSGIAAGAQVVTSAAPLPAWAEE"
     gene            complement(124..1224)
                     /locus_tag="OO544_003527"
     CDS             complement(124..1224)
                     /locus_tag="OO544_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HCR6769044.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKSFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPGLADRKPLWQYAEKLDDTYCAGCHAPIAADHYTVNAWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
     gene            complement(1612..2358)
                     /gene="cutC"
                     /locus_tag="OO544_003528"
     CDS             complement(1612..2358)
                     /gene="cutC"
                     /locus_tag="OO544_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HCR6769045.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(2372..2938)
                     /locus_tag="OO544_003529"
     CDS             complement(2372..2938)
                     /locus_tag="OO544_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR6769046.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSLRLGLNITPLTADHIS
                     LRCHQNATAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPG
                     EKRYPHEGWEHIEIVLLGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            3154..4890
                     /gene="argS"
                     /locus_tag="OO544_003530"
     CDS             3154..4890
                     /gene="argS"
                     /locus_tag="OO544_003530"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HCR6769047.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRHSAKVQFGDYQANGM
                     MAVAKKLGMTPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVSTPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGDEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKL
                     QSGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAK
                     GLAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRV
                     LYYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLA
                     DLLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNM
                     LAFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETL
                     TVVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLG
                     LDTLGIETVERM"
     gene            5067..5429
                     /locus_tag="OO544_003531"
                     /pseudo
     CDS             5067..5429
                     /locus_tag="OO544_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416391.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
     gene            complement(5433..6503)
                     /locus_tag="OO544_003532"
                     /pseudo
     CDS             complement(5433..6503)
                     /locus_tag="OO544_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
     gene            complement(6496..>7429)
                     /gene="flhB"
                     /locus_tag="OO544_003533"
     CDS             complement(6496..>7429)
                     /gene="flhB"
                     /locus_tag="OO544_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
                     /protein_id="HCR6769048.1"
                     /translation="DPNLILGQIILLIREAMLALLPLISGVVLVALISPVMLGGLVFS
                     GKSLQPKFSKLNPLPGIKRMFSAQTGAELLKAILKTILVGSVTGFYLWHHWPQMMRLM
                     AESPTTAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKKLRMSRQDIRDEFKQ
                     SEGDPHVKGRIRQMQRAAARRRMMADVPKADVIVNNPTHYSVALQYDENKMSAPKVVA
                     KGAGLVALRIREIGAENNVPTLEAPPLARALYRHAEIGQQIPGQLYAAVAEVLAWVWQ
                     LKRWRLAGGQRPVQPTHLPVPEALDFINEKPTHE"
BASE COUNT         1920 a         1947 c         1776 g         1786 t
ORIGIN      
        1 ctcttctgcc catgcgggga gcggtgctgc cgatgtcacc acctgcgctc cagcggcaat
       61 accgctgtgt ttaataaatt cacgtcttgt taatgtcata acttcctccc tgatcaacga
      121 ggatcactgt ttctcggtaa tatctttggc gttgtactgg aaataccgcg ttaaaatgtc
      181 cagttcgttt tcgctcatgc tggttcgtgc ccccattcct ttggcaatgg acggccacgc
      241 attgacggtg taatggtcgg cggcaatagg ggcatgacaa ccagcgcaat aggtatcgtc
      301 aagtttttca gcgtattgcc atagcggttt acggtccgct aatccgggat cggtaagcgc
      361 accctgtaaa gacgcctgac gccattgatt gccgtattcg tcagcctgcc attccccgtt
      421 taccgtgagc gccttgatac cttcttcact taatgtggct agcgccagcc gttgacctgc
      481 cgccaggtag agcgtgtttt cactgccctg catttgataa ccctgcaaca gaacgatcgg
      541 ctgtttgcca ctggcatcaa cgacggtgag atcggtacca ggattcacgg tagccagctc
      601 gcctatgcga gaagttttga aaggataaat atgtgcgcca ttagtcactg aagtagcggc
      661 ctgactttcc agctcatgcg ccgcgttgtc atccattttt atttctggcg gaaaatgggc
      721 aatgccttta tgacaatcga tacaggtttc gccgcccttt tgtgctttgt tatgcatttt
      781 ctgcgcagat tcactttgcg aggcaatatc catggcatca aaagaatggc aactacggca
      841 cgttgcagag tcagttgctt ttaattcttt ccatactgtt tcggccattt cctggcgatg
      901 agtttcgaac ttatcgtcac tgtctatttt gccgctaaca aattcatgat aaatatcttt
      961 agatgctttt aatttagcaa ataaataatc catccctgac tttggaatat ggcaatctgc
     1021 acattccgca cgtatccctt tctggttcga aaagtggaca gttccctgat attcctcaaa
     1081 agatttactc atcgagtggc aggaaaggca aaatgctgta tccgacgttt tatgtaaggc
     1141 tttttgcgcc agcaataacc cgccgccacc aaccacaacc gctatcagca aaaacaataa
     1201 cccaatgcgt tttttccctc gcatagattc ctctaaataa attccagaac accggaatca
     1261 ttgtataatt aaatacacaa cgaattaacc gcttgcataa ctcggcacaa cactgcctga
     1321 aataatcgat aaagaatatg atttattaca atgtaatcat taattgctaa ggaataaccc
     1381 agttgctatt tagaatatat atttatcagt tttagcaatt taaatcccat aatcaatgtg
     1441 aatataaaaa atattattag cacaccgcta tgttcactgt cacccgctca acagatcacg
     1501 gttatcgttc gttttttata ctgctcaggg ataaaaaaag gcccctgttg aaattgcagg
     1561 ggcctggtac gagcaagcat catattgggc gacatgatgc aacggtaaaa atcatttggc
     1621 ctgatggcgt tcaatgattc ctttcatttc agcaaccgcc gccccgtcta cgatataacg
     1681 cgaatactcg tccgcgtgtt catctgatga catggacaat ccttgattac gataacgcat
     1741 cggtgaggct tgccacgctc ccgcggagct atggacttcc agcactccgg catcgaggaa
     1801 gtggtgcagg ttttctgcac ggactcctgc tccggccata atgattggag catcacgatg
     1861 ggcaataagt tccataattt ttgataaacc ttgcagtgcg tctgattttt gccctgatgt
     1921 cagtacccgg gcaatgccca attccgcaag attattgagt gtatataaag ggttagcgca
     1981 catatcgaag gcgcgatgaa aagtcactgc cagcggaccg gcagcagcca ttattttttc
     2041 cattcgtggc atatcgacat tcccgtcaac atcgagaacg cccgtcacca gtccaggaaa
     2101 acctaattcg cggaccgtgc gcacatcctc aagaatggcg gcaaactcac cgtcgctgta
     2161 gcaaaaatca ccaccgcgtg ggcgaattat cggatgcaca gggatcgtca cccgctggcg
     2221 cacggatttc agtaccccca acgacggcgt taagcccccc tcttttgggg ctgcgcataa
     2281 ttcaactctg tctgcgccgt tttgctgcgc cgttagtgca cattccatgc tgtaacagca
     2341 aatttccagt aatgccattt ttgctcctta attacgctga ctgctcgctg gcaacgatct
     2401 cttcaatgga ccacggatga aacttaatag ttgttttgcc atcggtgacc gccagcgtcg
     2461 gattcggtaa tcgctcatgc tcaccttttg gtgaactggt tttaactgag atccccggta
     2521 ggctgagtcc ttcatcagaa agcaatgcca gcgcacgggc attcagcgtt tctggatcac
     2581 ccagcagaac gatttcaata tgttcccaac cttcgtgtgg gtaacgtttt tccccgggcc
     2641 acggtagctc cacaatagaa aactgccagt gcgcaacctg taccggttca tgcaatttaa
     2701 acagacaaat cggtctgcca ttgatcatat tttctgacaa aagctcgcca cactgttcaa
     2761 acccgcgacg ccagcgttca gcagtggcgt tttgatggca acgcaaagaa atgtgatcgg
     2821 cagtcagcgg agtgatattc aaccccagac ggagggaaag ttcatctaat gcgtgggtaa
     2881 atcgcggtaa atccgatgca atatcctgca gctcgtcgat agattgccag ttcgccataa
     2941 tcactcttcg tctttcagta aaagcgttaa tttaccctgt tgccctgcgc caaccaaccg
     3001 ctgatttcac gccgcttctg atgcaatagt gaaaacggca atacgccacg cgcacgttgc
     3061 tgacgaaaac agccatttgc agtatactcc cgccctaatt tctttaactg gtgcgggcaa
     3121 tttttgctcg cttcatcaat gtaaggtatt ccggtgaata ttcaggctct tctctcagaa
     3181 aaagtccgtc aggccatgat tgcggcaggc gcgcctgcgg attgcgaacc gcaggttcgt
     3241 cattcagcaa aagttcagtt cggcgactat caggctaacg gcatgatggc agttgctaaa
     3301 aaactgggta tgacaccgcg acaattagca gagcaggtgc tgactcatct ggatcttaac
     3361 ggtatcgcca gcaaagttga gatcgccggt ccaggcttta tcaacatttt ccttgatccg
     3421 gcattcctgg ctgaacatgt tcagcaggcg ctggcgtccg atcgtctcgg tgtttctacg
     3481 ccagaaaaac agaccattgt ggttgactac tctgcgccaa acgtggcgaa agagatgcat
     3541 gtcggtcacc tgcgctctac cattattggt gacgcagcag tgcgtactct ggagttcctc
     3601 ggtcacaaag tgattcgcgc aaaccacgtc ggcgactggg gcactcagtt cggtatgctg
     3661 attgcatggc tggaaaagca gcagcaggaa aacgccggtg atgaaatgga gctggctgac
     3721 cttgaaggtt tctaccgcga tgcgaaaaag cattacgacg aagatgaaga gtttgccgag
     3781 cgtgcacgta actacgtggt aaaactgcaa agcggtgacg aatatttccg cgagatgtgg
     3841 cgcaaactgg tcgacatcac catgacgcag aaccagatca cctacgatcg tctcaacgtg
     3901 acgctgaccc gtgatgacgt gatgggcgaa agcctctaca acccaatgct gccaggaatt
     3961 gtggcggatc tcaaagccaa aggtctggca gtagaaagcg aaggggcgac cgtcgtattc
     4021 cttgatgagt ttaaaaacaa ggaaggcgaa ccgatgggcg tgatcattca gaagaaagat
     4081 ggcggctatc tctacaccac cactgatatc gcctgtgcga aatatcgtta tgaaacactg
     4141 catgccgatc gcgtgctgta ttacatcgac tcccgtcagc atcaacacct gatgcaggca
     4201 tgggcgatcg tccgtaaagc aggctatgta ccggaatccg taccgctgga acaccacatg
     4261 ttcggcatga tgctgggtaa agacggcaaa ccgttcaaaa cccgcgcggg tggtacagtg
     4321 aaactggccg atctgctgga tgaagccctg gaacgtgcac gccgtctggt ggcagaaaag
     4381 aacccggata tgccagctga cgagctggaa aaactggcta acgcggttgg tattggtgcg
     4441 gtgaaatatg cggatctctc caaaaaccgc accacggact acatcttcga ctgggacaac
     4501 atgctggcgt ttgagggtaa taccgcgcca tacatgcagt atgcttacac gcgtgtattg
     4561 tccgtgttcc gtaaggcaga aattaacgaa gagcaactgg ctgcagctcc ggtaatcatc
     4621 cgtgaagatc gtgaagcgca actggcagct cgcctgctgc agtttgaaga aaccctcacc
     4681 gtggttgccc gtgaaggcac gccgcatgtg atgtgtgctt acctgtacga tctggctggt
     4741 ctgttctctg gcttctacga gcactgcccg atcctcagcg cagaaaacga agaagtgcgt
     4801 aacagccgtc tgaaactggc acaactgaca gcgaagacgc taaagctggg tctggatacg
     4861 ctgggtattg agacagtaga gcgtatgtaa tcgatttttc gtgagagtaa agcctgatca
     4921 gggttacctg atcaggcttt tttattgcca tctaaatgta ttctgaaaat ggacatgcca
     4981 ttgttttctg actgttggat aagaggccag aagcgtaata tccggcccca gggaaacgat
     5041 aacggttgaa tttaaggaat accgcagtgt ttaaatttct tgtattaaca ttaggcatta
     5101 tctcttgcca ggcttacgca gaagatacgg ttatagaaaa cgaccatgac atttcagcca
     5161 tcaaagattg ttggcaaaaa aattcagatg atgatactga cattaacgtg atcaaatcat
     5221 gcctgcgaca agaatacaat ctcgtcgatg cgcaattaaa taaagcctat ggtgaagctt
     5281 atcgttatat agaacaagtg ccacgcacag gtgtaaaaaa acctgatacc gaacaactta
     5341 acttgcttaa aaaatcacag cgagcctggc tggattttag ggacaaagaa tgtgaattaa
     5401 tcctttcaaa tgaggacgtt caggatttaa tgtacatcgt tccacgtcgc ttcgacaacg
     5461 gtattattct ctgccatttt taccggtttg ggttcggcag gcgctttttg ttcgcgtccg
     5521 cgtatccacc aggccagccc gagcaatccg gcagtgaaca gcaaaaatac caggttcggc
     5581 attccaggca ccaggccgag taaaccgagc acggcggcgc ttaacaacat aacgcttggg
     5641 ttactgaaaa gctgattcac catctgctcg ccaacatcct gatcggtgct gacacgcgta
     5701 acgatgaccc ccgcggcggt aaaaatcacc agcgccggaa tttgtgccac cagaccatcg
     5761 ccaatggtca atagcgtata actttccgcc gcgtgtccca tgctcatgcc atgttgcagc
     5821 acgccgacca gcaacccgcc gacaacgtta atgaccatga tgaggatccc ggcgatggca
     5881 tcgccgcgaa caaacttact tgccccgtcc attgagccgt aaaaatcggc ttcctgagtc
     5941 acttcggagc ggcgtttttt cgcctcatct tcaccaatca atccagcgtt aaggtcggcg
     6001 tcaatcgcca tctgcttacc cggcatacca tcgagaacaa agcgcgcacc cacttctgcg
     6061 atacgcccgg cacctttggt aatgaccata aagttgatga tcacgagaat gacaaacacc
     6121 acgataccga tagcgaaatt gccaccaacg aggaagtgac cgaacgcttc gaccaccttc
     6181 cctgccgccg ccgcgccggt atgcccttcc atcaaaatga tacgggttga agccacgtta
     6241 agtgccagac gcaacaacgt agtaaacaac agaatggtcg gaaacgcagc aaactcaagc
     6301 gtgcgctggg taaacatcgc caccagcaac accatgatcg acaaggcaat attgaaggta
     6361 aacaacaggt cgagtatgaa tgcgggcagt ggcagcacca tcatcgacaa gatcaacagg
     6421 atcaaaatcg gtccggcaag gatctgccat tgtgtcgatt tcaggtttgc gggcaggcgc
     6481 agcatcgcgg ccagattact catgggtcgg tttctcgtta ataaaatcca gggcttccgg
     6541 caccggaaga tgagtaggtt gtacagggcg ctgtccacca gccagacgcc agcgtttcag
     6601 ttgccagacc caggccagca cttccgccac cgcggcgtac agttgacccg ggatttgttg
     6661 accaatctcc gcatgtcgat acagcgctcg cgccagcggc ggcgcttcaa gcgtcgggac
     6721 gttattttca gcgccaattt cacgaatgcg cagcgcgacc agccctgcac ctttagcgac
     6781 cactttcggt gcgctcattt tgttttcgtc atactgcaac gctaccgaat agtgggtcgg
     6841 gttattgaca atgacatcag ctttcggcac atcggccatc atccgacgcc gtgcagcagc
     6901 tcgctgcatc tgacggatcc gccctttaac atgagggtca ccttcgcttt gtttgaactc
     6961 atcacgaata tcctgccgcg acatacgcag ctttttcagg tggctgaaga tttggaaaaa
     7021 gacgtcaaat cccaccattg gaatgacacc aagcaccacc agcagtgcgc atagcccaac
     7081 cagatccatc gcattaccca tggcggtagt cggagactcg gccattaagc gcatcatctg
     7141 cggccagtga tgccagagat aaaaccccgt cacgctgcca accaggatgg ttttcaaaat
     7201 tgccttaagc aactccgcgc cagtctgtgc cgagaacatc cgtttaatgc ccggtagcgg
     7261 gttgagtttg gaaaacttcg gctgcaagga tttgccgcta aataccagcc cccccagcat
     7321 gaccggagaa atgagcgcca ccagcaccac gccgctaatc agcggcagca gcgccagcat
     7381 ggcttctctg atcagcagaa taatctgccc gaggatcaga ttcgggtca
//
LOCUS       DAJTZL010000184         7413 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.184, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000184 DAJTZL010000000
VERSION     DAJTZL010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7413
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.184"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(73..1581)
                     /gene="putP"
                     /locus_tag="OO544_003534"
     CDS             complement(73..1581)
                     /gene="putP"
                     /locus_tag="OO544_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HCR6769049.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALLAGAASTILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPAVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKQASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQEN"
     gene            2003..5965
                     /gene="putA"
                     /locus_tag="OO544_003535"
     CDS             2003..5965
                     /gene="putA"
                     /locus_tag="OO544_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HCR6769050.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLENLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLVDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQPVAAGEMSPVINPAEPKDIVGFVREATPR
                     EVEQALESAVNNAPIWFATPPAERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGQGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVVDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRPVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELHKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSAVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            complement(6005..6643)
                     /gene="rutR"
                     /locus_tag="OO544_003536"
     CDS             complement(6005..6643)
                     /gene="rutR"
                     /locus_tag="OO544_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HCR6769051.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEIFFNQTVENVQRIIIEGIRPR"
     gene            6931..>7413
                     /locus_tag="OO544_003537"
     CDS             6931..>7413
                     /locus_tag="OO544_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HCR6769052.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPVYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     D"
BASE COUNT         1771 a         2054 c         1943 g         1645 t
ORIGIN      
        1 tatatggcga ggcacattaa gggagcgcaa cccgctgctg aagccaaaca aaaccgccgc
       61 aggctaagtc ccttagtttt cctgcaaccg tgacggcgga gccgaatgat agtgcgcatc
      121 ggcctcggca aagcgttttt gcatcgccgc tgacggcgct ttacccagca aactaaacac
      181 tacaatcccg atactgccga agatgaagcc cggaataatt tcgtacagtc ccagccagcc
      241 gaactgcttc cagacgataa ctgtcagcgc accgatgatc atccccgcca gcgcaccgtt
      301 gcgcgtcatg cgtgaccaca tcaccgagaa cagcaccacc ggaccaaacg ccgcgccaaa
      361 gcctgcccac gcgtagctca ctaagcccag cacgcggttt tccgggtttg ccgccagcgc
      421 aatcgccacc agcgccacca ccagcaccat cacacgtcct acccacacca gctctttctg
      481 gctggcctgt ttacgcagaa acgctttgta taaatcttcg gtaatcgcac tggagcacac
      541 cagcagctgg caacttaagg ttgacattac cgccgccaga atcgccgaca gcagaatccc
      601 ggcaatccac gggttaaaca gaatttgcgc cagttcgata aacacgcgtt cagcgttctg
      661 gtttaccgca cctgctaccg ccggatgctc gttaaagtaa gcgataccaa agaagccaac
      721 agccaccgcc cctgccaggc aaaggatcat ccaggtcata ctgatacgac gcgcatggac
      781 aatgctgtgg tgagaatctg ccgccataaa acgcgccagg atgtgcggct gcccgaagta
      841 acccagcccc caacccatca atgagataat ggcaacaaag ttcagccctt tgagcatatc
      901 cacgttttcg atgctctttt gtttgatcac ttccagcgag tcaccaaagc caccgacact
      961 gataatgacg ataaccggcg tcaggatcag ggcaaaaatc atcaggctgg cctgtacagt
     1021 gtcagtccag ctcaccgcga ggaaaccgcc aataaaggtg taaaggatcg tcgacgcagc
     1081 tcccgccaac agagccgttt cgtaactcat gccaaaggta ctttcaaaca gacgcgcgcc
     1141 tgccacaatg cccgaagcgc aataaatggt gaagaacagc aaaataacca gcgcggagat
     1201 aatgcgcaaa atgcggcttt tatcttcaaa acgcccggtg aaataatccg gcaatgttaa
     1261 ggcgttatta ttgtattcgg tatgcacacg caaccgcccg gccaccagct tccagttgat
     1321 ccacgcgcct aatgtcaggc caatggcgat ccagctttcg gaaatcccgg aaagaaaaac
     1381 cgcgccaggc aaccccatta acagccagcc gctcatatcc gacgcacccg ccgataatgc
     1441 cgtcacgaat ggcccaagac tacgaccgcc cagaatatag tcgtcaaagt ttttcgttga
     1501 tcgccaggcg ataaacccaa tcaatatcat gccaaagata tagacacaaa atgtcaccaa
     1561 catcggtgtg ctaatagcca tctaaagtct ccaaaaaatt attatcggca atgtcgaaac
     1621 ttgccgttat atctgccacc ggaacggggt aacagagttt atgttttacc agggcgaccg
     1681 tatcctgccg gaagcgctgg ttattcacaa tcgatttaac acaccattta cattaaattt
     1741 tagtgctcag cgacactatt tttcatcagg ttgcactctc tcacattttt tgcggttgca
     1801 cctttcaaaa aggttaactg ccgcagagaa aaagtctgag ttatttttta atcactgtca
     1861 tatcgatttc ttttattaac atttcattca tttttaagct tgctacgcaa gtcacattta
     1921 acatggttgc acaaagttgc aacatcatgg atatttcacg ataacgttaa gttgcacctt
     1981 tcagaacaac aggagtaatg gcatgggaac caccaccatg ggggttaagc tggacgacgc
     2041 gacgcgtgag cgtattaagt ctgccgcgac acgtatcgat cgcacaccac actggttaat
     2101 taagcaggcg attttttctt atctcgaaca actggaaaac agcgatactc tgccggagct
     2161 acctgcgctg ctttctggcg cggccaatga gagcgatgaa gcaccgactc cggcagagga
     2221 accacaccag ccattcctcg actttgccga gcaaatattg ccccagtcgg tttcccgcgc
     2281 cgcgatcacc gcggcctatc gccgcccgga aaccgaagcg gtttctatgc tgctggaaca
     2341 agcccgcctg ccgcagccag ttgctgaaca ggcgcacaaa ctggcgtatc aactggccga
     2401 taaactgcgt aatcaaaaaa atgccagtgg tcgtgcagga atggtccagg ggttattgca
     2461 ggagttttcg ctgtcatcgc aggaaggcgt ggcgctgatg tgtctggcag aagcgttgtt
     2521 gcgtattccc gacaaagcca cccgcgacgc gttaattcgc gacaaaatca gcaacggtaa
     2581 ctggcagtca cacattggtc gtagcccgtc actgtttgtt aatgccgcta cctgggggct
     2641 gctgtttact ggcaaactgg tttccaccca taacgaagcc agcctctccc gctcgctgaa
     2701 ccgcattatc ggtaaaagcg gtgaaccgct gatccgcaaa ggtgtggata tggcgatgcg
     2761 cctgatgggc gagcagttcg tcacgggtga aaccatcgcg gaagcgttag ccaatgcccg
     2821 caagctggaa gagaaaggtt tccgttactc ttacgatatg ctgggcgaag ctgcactgac
     2881 tgccgcagac gcacaggcgt atatggtttc ctatcagcag gcgattcacg ccattggtaa
     2941 agcgtctaac ggtcgtggca tctatgaagg tccgggcatt tcaatcaaac tgtcggcgct
     3001 gcatccgcgc tacagccgcg cccagtatga ccgggtaatg gaagagcttt acccgcgtct
     3061 gaaatcactc accctgctgg cgcgtcagta tgatattggt attaacatcg atgccgaaga
     3121 ggccgatcgt ctggagatct ccctcgatct gctggaaaat ctctgtttcg agccggaact
     3181 ggcaggctgg aacggcatcg gttttgttat tcaggcttat caaaaacgct gcccgttggt
     3241 gatcgattac ctggttgatc tcgccacccg cagccgtcgc cgtctgatga ttcgcctggt
     3301 gaaaggcgcg tactgggata gtgaaatcaa gcgtgcgcag atggacggcc ttgaaggcta
     3361 tccggtttat acccgcaagg tgtataccga cgtttcttat ctcgcctgtg cgaaaaagct
     3421 gctggcggtg ccgaatctaa tctacccgca gttcgcgacg cacaacgccc atacgctggc
     3481 agcgatttat caactggcag gtcagaacta ctatccgggt cagtacgagt tccagtgcct
     3541 gcatggtatg ggcgagccac tgtacgagca ggtcaccggg aaagttgccg acggtaaact
     3601 taaccgaccg tgtcgtattt atgccccggt tggcacacat gaaacgctgc tggcgtatct
     3661 ggtgcgtcgc ctgctggaaa acggtgctaa cacctcgttt gttaaccgta ttgccgacac
     3721 ctctttgcca ctggatgaac tggtcgccga tccggtcact gctgtagaaa aactggcgca
     3781 acaggaaggg caaactggat taccgcatcc gaaaattccc ctgccgcgcg atctttacgg
     3841 tcacgggcgc gacaactcgg cagggctgga tctcgctaac gaacaccgtc tggcctcgct
     3901 ctcctctgcc ctgctcaata gtgcactgca aaaatggcag gccttgccaa tgctggaaca
     3961 accggtagcg gcaggtgaga tgtcgcccgt tattaaccct gcggaaccga aagatattgt
     4021 gggctttgtg cgtgaagcca cgccacgtga agtagaacag gcgctggaaa gtgcggttaa
     4081 taacgcgcca atctggtttg ccacgcctcc ggctgaacgc gcggcgattt tgcatcgcgc
     4141 tgccgtgcta atggaaagcc agatgcagca actgattggt attctggtgc gtgaagccgg
     4201 aaaaaccttc agtaacgcca ttgccgaagt gcgcgaagcg gtcgattttc tccactacta
     4261 cgccggacag gtgcgggatg atttcgctaa cgaaacccac cgtccattag ggcctgtggt
     4321 gtgtatcagt ccgtggaact tcccgctggc tattttcacc gggcagatcg ctgccgcact
     4381 ggcggcaggt aacagcgtgc tggcaaaacc ggcagaacaa acgccgctga ttgccgcgca
     4441 agggatcgcc attttgctgg aagcgggtgt accccctggc gtggtgcaat tgctgccagg
     4501 tcagggggaa accgtgggcg cgcaactgac gggtgatgat cgcgtgcgcg gggtgatgtt
     4561 taccggttca accgaagtcg ctacgttact gcagcgcaat atcgccagcc gcctggacgc
     4621 tcagggtcgc cctattccgc tcatcgctga aaccggcggc atgaacgcga tgattgtcga
     4681 ttcttcagca ctgaccgaac aagtcgtcgt ggatgtgctg gcctcggcgt tcgacagtgc
     4741 gggtcagcgt tgttcggcac tgcgcgtgct gtgcctgcaa gatgagattg ccgaccacac
     4801 gttgaaaatg ctgcgcggtg caatggccga atgccggatg ggtaatccgg gtcgcctgac
     4861 caccgatatc ggtccggtga ttgatagcga agcgaaagcc aatattgagc gccatattca
     4921 gaccatgcgt agcaaaggcc gtccggtgtt ccaggcggtg cgggaaaaca gcgaagatgc
     4981 ccgtgaatgg caaagcggca cctttgtcgc cccgacgctg atcgaactgg atgactttgc
     5041 cgaattgcac aaagaggtct ttggtccggt gctgcatgtg gtgcgttaca accgtaacca
     5101 gctaccagag ctgatcgagc agattaacgc ttccggctat ggtctgacgc ttggtgtcca
     5161 tacgcgtatt gatgaaacca tcgcccaggt cactggctcg gcccatgttg gtaacctgta
     5221 tgttaaccgt aatatggtgg gcgcagtggt tggtgtgcag ccgttcggcg gcgaagggtt
     5281 gtccggtacc gggccgaaag caggcggtcc gctctatctc taccgtctgc tggcgaatcg
     5341 cccggaaagt gcgctggcag tgacgctcgc gcgtcaggat gcagagtatc cggtcgatgc
     5401 acagttgaaa gccgcattga ctcagccgct aaatgcactg cgggaatggg cagcaaatcg
     5461 tccagaattg caggcgttat gtacgcaata tggcgagctg gcgcaggcag gaacacaacg
     5521 attgctgccg gggccgacgg gtgaacgcaa cacctggacg ctgctgccgc gtgagcgcgt
     5581 gttgtgtatt gccgatgatg agcaggatgc gctgactcag ctcgccgccg tgctggcggt
     5641 gggcagccag gtactgtggc cggatgacgc gctgcatcgt cagctagtaa aggcattgcc
     5701 atcggcagtc agcgaacgta ttcaactggc gaaagcggaa aatataaccg ctcaaccctt
     5761 tgatgcggtg atcttccacg gtgattcgga tcagcttcgc gcattgtgtg aagccgttgc
     5821 tgcgcgggat ggcgcaattg tttcggtgca gggttttgcc cgtggcgaaa gcaatatcct
     5881 tctggaacgg ctgtatatcg aacgttcgct gagtgtgaat accgctgccg ctggcggtaa
     5941 cgccagttta atgacgatcg gttaaacagg tagccggagg atgttacaac ctcctccggc
     6001 atctttaacg tggtcgaatc ccctcaataa taatccgctg cacgttttca accgtttgat
     6061 tgaaaaatat ctcatcgcgc aacgtcgcgc ctgtcaccgc ctccacctga ggggcgaaat
     6121 cggcgtaatg ttgagtggaa gcccaaatca tgaaaatcaa atgctgcgga tcaatcggcg
     6181 cgagtttgcc gcttttgacc caaccggcaa tcagcgcaga tttctcatca attaatgcct
     6241 tcaaatcgcc cgtcagttca tccattaaca gcggcgcgcc tgccagcatc tccatacaga
     6301 acaggcgcga agcctgcgga taatcgcggg agacttccag cttcagacgg atgtactctt
     6361 ttatcgccgc cagcggagcg aaatcttcac gaaacgcttt taacggtgcc agccagatat
     6421 cgagaatctg ccgcagcacg gcaatataca gcgcctcttt tgacggaaag taatacagca
     6481 gattggtttt tgaaacaccc gccaactctg cgatctgctc cagccttgtg ccgtgaaaac
     6541 cgaattgtga aaaagtgtcc agtgctgcgc taagaatcgc ttttttcttc gcgcttactg
     6601 cgcgcgaacg tttacccgtt gttttcactg cgccttgcgt catgcgctct cccctcttgt
     6661 agattggcaa caggatagca aaacccccgc ccggcctcga ccttctgcac tctcatcgcg
     6721 cacagatgca tgttttatgt gcaactgttt tgaccgttta gtccactttt tatcagatat
     6781 tttagataac gtaatattaa catattaata acattaactt tttaaaaact ggcatccgct
     6841 ttgcaaacaa gccaatacgc ggctgaaaag gatatgcagg atgcagcgcc ccgtctgact
     6901 ttcactttac gagacgaaga gaggttgatg atgaaaattg gcgtattcgt acctattggc
     6961 aacaacggct ggctcatttc gacccacgcg ccgcagtaca tgccgacctt tgaactgaat
     7021 aaagccattg tgcagaaagc ggagcattac catttcgatt tcgccctgtc gatgatcaaa
     7081 ctgcgtggct ttggcggcaa aactgagttc tgggatcaca accttgagtc gttcaccttg
     7141 atggcgggtc tggcagccgt gacctcgcgc attcagattt acgccactgc tgccacctta
     7201 acgttacctc cggcaatcgt cgcccgtatg gccgcaacca tcgactccat ctctggcggg
     7261 cgttttggcg tcaacctcgt gactggctgg caaaagcccg tgtatgagca gatgggtatc
     7321 tggcctggcg atgactattt ctcccgtcgt tacgactatc tcaccgaata tgttcaggtg
     7381 ctgcgcgacc tgtggggcac ggggaaaagc gat
//
LOCUS       DAJTZL010000185         7239 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.185, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000185 DAJTZL010000000
VERSION     DAJTZL010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7239
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.185"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..337
                     /locus_tag="OO544_003538"
     CDS             <1..337
                     /locus_tag="OO544_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR6769053.1"
                     /translation="ADLSRYSGTVVNRPTFFAFAKGAGVMGEAGPEAILPLRRGADGK
                     LGGVADTGGSGMVMFAPQYNIEINNDGTNGQIGPAALKVVYDLGKKAAADFMQQQARD
                     GGRLSGAYR"
     gene            337..666
                     /locus_tag="OO544_003539"
     CDS             337..666
                     /locus_tag="OO544_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6769054.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSTY
                     SVKIRVRKGEHPFLKAFLERHGGVRAFQWTPPYDWKPIRVVCRKWSASVGALWVTITA
                     DFEQVVA"
     gene            676..1374
                     /locus_tag="OO544_003540"
     CDS             676..1374
                     /locus_tag="OO544_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HCR6769055.1"
                     /translation="MQDIPQETHHETTRLTQSAQVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGTGFELNGKGSAARPTLTVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLATPTETDG
                     AVFPGRIMLANTCMWTYRSDECGYTGRAVADEFDKPTTDIRKDKCSKCMRGCELRNNT
                     GNFGGFLSINKLSQ"
     gene            1379..2122
                     /locus_tag="OO544_003541"
     CDS             1379..2122
                     /locus_tag="OO544_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6769056.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVRTPEGDRYLPSENISGEPE
                     ERFRMAPEDWLRAQMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRNGQNLYLDNMEVT
                     GFYRVALTEAQPGDVLLCCFGSSVPNHAAIYCGDGGLLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWRASAFTGIYNDLVAASTFV"
     gene            2059..2661
                     /locus_tag="OO544_003542"
     CDS             2059..2661
                     /locus_tag="OO544_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HCR6769057.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAIRALATQLPAFRQKLSD
                     GWYQVRIAGRDAGETELSARLNEPLANGAVIHIVPRLVGAKSGGVFQAVLGAAVMAVA
                     IWMPGVGIMASNLLFSLGASMTLGGVAQMLAPKARTPRTQTTDNGKQNTYFSSLDNMV
                     AQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            2722..6201
                     /locus_tag="OO544_003543"
     CDS             2722..6201
                     /locus_tag="OO544_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HCR6769058.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVDGPVDGLKS
                     VLLNGTPVLDSEGKTNFSGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRLTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVGNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGNQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVTDGFGGTEPRITCNSYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQMLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPDNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGSGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDRRGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVAADDGSERLVSTARTTETTYRFRQ
                     LALGNYSLTVRAVNARGQQGDPASVSFRIAAPAAPVTIELIPGYFQITAVPKLAVYDP
                     TVQFEFWFSEKRIADIRQVETSARYLGTALYWIAASINIRPGHDYYFYVRSVNTVGKS
                     AFVEAIGRASDDAEGYLNFYKGLINKTHLGKELWTQIDNGQLAPDLTEIRTSITNVSN
                     EITQTVNKKLENQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMKDGRLYIAGIGAGIEN
                     TPAGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNDVFLKCLTAPTITSGGNP
                     PAFSLTPGGRLTAKNADISGNVNANSGTLNNVTINKNCRVLGKLSANQIEGDLVKTVG
                     KPFPRDSRAPERWPSGTITVRVYDDQPFDRQIVIPAVAFRGAKHERKNNNIYSSCRLI
                     VKKNGAEIYNRTTLDNTLIYTGVIDMPAGHGHMTLEFSVSAWLVNGWYPTASISDLLV
                     VVMKKATAGITIS"
     gene            6269..6868
                     /locus_tag="OO544_003544"
     CDS             6269..6868
                     /locus_tag="OO544_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HCR6769059.1"
                     /translation="MRKVCAAILSAAICLAVSGAPAWASEHQSTLSAGYLQTHTDMPG
                     SDDLKGINVKYRYEFTDTLGLITSFSYANAEDEQKTHYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDGGRHSNTSLAW
                     GAGVQFNPTESVAIDLAYEGSGSGDWRTDGFIVGVGYKF"
     gene            6932..>7239
                     /locus_tag="OO544_003545"
     CDS             6932..>7239
                     /locus_tag="OO544_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004981847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCR6769060.1"
                     /translation="MAVKISGVLKDGAGKPVVNCAIELRARRTSPTVVAHVVATCVTD
                     NNGAYVIEAEPGYYEVALHCNGWQPTRVGDIDVAPTDAPGTLNAFLNAPKDGDLRPEV
                     "
BASE COUNT         1637 a         1726 c         2315 g         1561 t
ORIGIN      
        1 tgctgatttg agtcgctaca gtggcacggt ggttaaccgt ccgacgtttt ttgcttttgc
       61 aaaaggcgcg ggtgtgatgg gggaagcggg acctgaagcc attctgccac tgcgtcgcgg
      121 tgctgatggt aagctggggg gtgtggcgga tactggtggt tcaggcatgg tgatgtttgc
      181 cccgcagtac aacattgaga tcaataacga tggcacgaac gggcagatag gtccggctgc
      241 cctgaaggtg gtttatgacc tcgggaaaaa agcggccgcg gactttatgc aacagcaggc
      301 ccgtgatggt ggccggttaa gtggagcata ccggtaatgg agacgtttca ctggaaagtg
      361 cgcccggata tgaatgtggt atcagagccg aaagtggtga cagtgaagct gggcgatggt
      421 tatgaacagc gtcgtgcggc gggactgaat aaccagttgt cgacttacag cgtgaagata
      481 cgtgttcgta aaggtgaaca cccattttta aaagcctttc tggaacggca cggtggcgtc
      541 cgcgcatttc agtggacgcc accttatgac tggaagccga tcagggtggt ttgtcgtaaa
      601 tggtcggcaa gcgtgggggc gttgtgggta accataacgg cagattttga acaggtcgtg
      661 gcataggagg ctctgatgca ggatattcca caggaaacac atcatgagac gacacgcctc
      721 actcagtcag cccaggtggt gctctgggaa atcgatctga cagaggtcgg tggtgaacgt
      781 tattttttct gtaatgagca gaacgaaaaa ggtgagccgg ttacctggca ggggcggcag
      841 tatcaggcat accccattca ggggacggga tttgaactga acggcaaggg cagtgctgcc
      901 cgtccgacac tgacggtttc taacctgcac ggaatggtca ccgggatggc ggaagacctg
      961 cagagtctgg tcggcggaac ggtggtcagg cgtaaggttt acgcccgttt tctggatgcg
     1021 gtgaacttcg tcaacggaaa cagcgacgcc gatccggaac aggaggtgat cagccgctgg
     1081 cgcatcgagc agtgcagcga actgagcgcg gtcagtgcct cttttgtgct ggccacacca
     1141 acggagacgg atggcgcggt tttcccgggg cgtatcatgc tggcgaatac ctgtatgtgg
     1201 acttaccgtt ctgatgagtg tggttacacg ggcagggcag tggctgacga gttcgacaaa
     1261 ccaacgacgg atatccggaa ggacaaatgc agcaagtgta tgcgcgggtg tgagttgcgc
     1321 aacaataccg gtaatttcgg cggtttcctt tccatcaata aactttctca gtaaatccat
     1381 gacacagaca gaatcagcga ttctggcgca cgcccggcga tgtgcgccag cggagtcgtg
     1441 tggcttcgtg gtaagaacgc cggagggaga caggtatctt cccagcgaga atatctccgg
     1501 tgagccggag gaacggttcc ggatggctcc ggaggactgg ctgcgggcac aaatgcaggg
     1561 tgagattgtg gcgctggtcc acagtcatcc cggtggtctg ccctggctga gtgaggctga
     1621 ccggcggctg caggtgcaga gtgatttgcc gtggtggctg gtctgccggg gggcgattca
     1681 caagttccgc tgtgtgccgc atctcaccgg gcggcgcttt gagcacgggg tgacggactg
     1741 ttacacgctg ttccgggatg cttatcatct ggcagggatt gagatgccgg attttcatcg
     1801 cggggatgac tggtggcgta acggtcagaa tctctatctt gacaatatgg aggtgacggg
     1861 tttttaccgt gtcgcactga cagaggcaca gccgggcgat gtgctgctgt gctgttttgg
     1921 ttcatcggtg ccgaatcatg ccgccattta ctgcggtgac ggcgggctgc tgcaccatat
     1981 tcctgaacaa ctgagcaaac gagagaggta caccgacaaa tggcagcgac gcacacactc
     2041 cctctggcgt caccgggcat ggcgcgcatc tgcctttacg gggatttaca acgatttggt
     2101 cgccgcatcg accttcgtgt gaaaacgggg gctgaagcca tccgggcact ggccacacag
     2161 ctcccggcgt ttcgtcagaa actgagtgac ggctggtatc aggtgcgcat tgccgggcgt
     2221 gatgcaggtg aaaccgaatt gtctgcccgt cttaatgagc cgctggcaaa tggtgcagtg
     2281 atccacatag taccgcgtct ggtgggagct aaaagtggcg gtgtgtttca ggcggtgctg
     2341 ggggcggctg ttatggcggt tgctatatgg atgccggggg taggaattat ggcgagtaat
     2401 ctgctgtttt ctctcggtgc cagtatgacg cttggcggtg ttgcacagat gctggcaccg
     2461 aaagccagaa ctccccgtac acagacaacg gataacggta agcagaacac gtatttctcg
     2521 tcactggata acatggttgc ccagggcaat gttctgcccg ttctgtacgg tgaaatgcgc
     2581 gtggggtcac gtgtggtttc tcaggagatc agcacggcag acgaagggga tggtggtcag
     2641 gttgtggtga ttggtcgctg atgcaaaatg ttttatgtga aaccgcctgc gggcggtttt
     2701 gtcgtttatg gagcgtgagg aatgggtaaa ggcagcagta aggggcatac cccgcgcgaa
     2761 gcgaaggaca acctgaaatc cacgcagttg ctgagtgtga ttgatgccat cagcgaaggg
     2821 ccggttgacg gtccggtgga tggattaaaa agcgtgctgc tgaacggtac gccggtcctg
     2881 gacagcgagg ggaagacaaa cttttccggt gttacggtgg tgttccgtgc aggtgagcag
     2941 gagcagacac cgccggaggg ttttgaatcc tccggctccg agacggtgct gggtacggaa
     3001 gtgaaatatg acacgccgat cacccggacc atcacgtcgg caaacatcga ccgtctgcgc
     3061 ctgaccttcg gtgtgcaggc actggtggaa accacctcaa agggtgaccg gaatccgtcg
     3121 gaagtccgcc tgctggttca gatacagcgt aacggtggct gggtgacgga aaaagacatc
     3181 accattaagg gcaaaaccac ttcgcagtat ctggcctcgg tggtggtggg taacctgccg
     3241 ccgcgcccgt ttaatatccg gatgcgcagg atgacaccgg acagcaccac agaccagctg
     3301 cagaacaaaa cgctctggtc gtcgtacacc gaaatcatcg atgtgaaaca gtgctacccg
     3361 aacacggcgc tggtcggcgt gcaggtggat tcagagcagt tcggtaacca gcaggtgagt
     3421 cgcaattatc atcttcgcgg gcgcattctg caggtgccgt cgaactataa cccgcagacg
     3481 cggcaataca gcggtatctg ggacggaacg cttaagccag catacagcaa caacatggcc
     3541 tggtgtctgt gggacatgct cactcatccg cgctacggca tggggaaacg tcttggtgcg
     3601 gcagatgtgg acaaatgggc gctgtatgtc atcggccagt actgcgacca gtcagtgacg
     3661 gacggatttg gcggcacgga gccgcgcatc acctgtaact cttacctgac cacacagcgt
     3721 aaggcgtggg atgttctcag tgatttctgc tcggcgatgc gctgtatgcc ggtatggaac
     3781 ggacagatgc tgacgttcgt gcaggaccga ccgtcggata aggtgtggac ctataaccgc
     3841 agtaatgtgg tgatgccgga cgatggcgcg ccgttccgct acagcttcag cgccctgaag
     3901 gaccgccata atgccgttga ggtgaactgg attgacccag ataacggctg ggagacggcg
     3961 acagagcttg tggaggatac gcaggccatt gcccgttacg gtcgtaatgt cacgaagatg
     4021 gatgcctttg gctgtaccag ccgggggcag gcacaccgag ccgggctgtg gctgattaaa
     4081 acggaactgc tggaaacgca gaccgtggac ttcagcgtgg gcgcagaagg gcttcgccat
     4141 gtaccgggtg atgtcattga aatctgcgat gatgattatg ccggtatcag caccggtggt
     4201 cgcgtactgg cggtgaacag ccagacccgg acgctgacgc tcgaccgtga aatcacgctg
     4261 ccgtcctccg gtaccacgct gataagcctg gttgacggaa gtggcaatcc ggtcagcgtg
     4321 gaggtccagt ccgtcaccga cggcgtgaag gtgaaagtga gccgggttcc tgacggcgtt
     4381 gcagaataca gcgtgtgggg gctgaagctg ccgacgctgc gccagcgtct gttccgctgc
     4441 gtgagtatcc gtgagaacga cgacggcacg tatgccatca ccgccgtgca gcatgtgccg
     4501 gaaaaagagg ccatcgtgga taacggggca cactttgacg gtgaccggcg cggcacggtg
     4561 aatggtgtca cgccgccagc ggtgcagcat ctgaccgccg aagtcaccgc agacagcggg
     4621 gaatatcagg tgctggcgcg atgggacacg ccgaaggtgg tgaagggtgt gagcttcctg
     4681 cttcgcctga ccgtggcagc ggacgacggc agtgagcggc tggtcagcac agccaggacg
     4741 acggaaacca cataccgctt caggcagctg gcgctgggga attacagtct gacagtccgg
     4801 gcggtaaatg cccgggggca gcagggcgat ccggcgtcgg tatcgttccg gattgcggca
     4861 ccggcagcgc ctgtcactat tgaactgata ccggggtatt ttcagataac ggcggtcccg
     4921 aaactggctg tatatgaccc gacggtgcag tttgagttct ggttttcgga aaagcggatt
     4981 gcggatatca ggcaggttga aaccagcgcc cgctatcttg gcacggcgct gtactggata
     5041 gccgccagta tcaatatcag gccgggccat gattattatt tttacgttcg cagtgtgaac
     5101 accgttggca aatcggcatt tgtggaggcc atcggtcggg cgagcgatga tgcagaaggt
     5161 tatctgaatt tttataaagg gttgatcaat aaaacgcatc tcggcaagga gttgtggacg
     5221 cagattgata acggtcagct tgcgccggac ctgactgaaa tcaggacgtc cattacgaat
     5281 gtcagcaatg aaatcacgca aaccgtcaat aaaaaactgg aaaatcagag tgctgcaatc
     5341 cagcagatac agaaagttca ggttgataca aataataacc tgaacagcat gtgggctgtg
     5401 aagctgcaac agatgaagga cggacgcctt tatattgcgg gtatcggtgc cggtattgag
     5461 aatacgccag caggaatgca gagtcaggtg ctgctggcgg cagacaggat tgcgatgatt
     5521 aatcctgcga atggcaacac aaagccgatg tttgttggtc agggcgatca gatattcatg
     5581 aacgacgtgt tcctgaaatg cctgacggct ccgaccatta ccagcggcgg taatcctccg
     5641 gcattttccc tgacacctgg cggacggctg acggcgaaaa atgccgatat cagcggtaac
     5701 gtgaacgcga actccgggac gctcaacaac gtcacgatta ataagaactg tcgggttctg
     5761 ggaaaattgt ccgcgaacca gattgaaggc gatctcgtta aaacagtggg caaacctttc
     5821 ccacgggact cccgggcacc ggagaggtgg ccatcaggga ccattaccgt cagggtttat
     5881 gacgatcagc cgtttgatcg gcaaattgtt attcccgcgg tggcgtttcg cggtgctaaa
     5941 catgagcgga agaataacaa tatttattcg tcatgccgcc tgatagtgaa gaaaaacggt
     6001 gctgaaattt ataaccgaac gaccctggat aatacgctga tatatacggg tgttattgat
     6061 atgcctgccg gtcacggtca catgacgctg gagttttctg tatcggcatg gctggtaaat
     6121 ggctggtatc ccacagcaag tatcagcgat ttgctggttg tggtgatgaa gaaagccact
     6181 gcaggcatca cgattagctg aattttataa cccagatacg ggcaccagaa atggtgcctt
     6241 ttttattgca gaaaagcgag aggtaattat gcgtaaagtt tgtgcagcca ttttgtccgc
     6301 agccatctgt ctggccgtat ccggtgcgcc tgcatgggcg tctgaacatc agtccacgct
     6361 gagcgccggg tatcttcaga cccatactga tatgccaggc agcgatgacc tgaagggcat
     6421 taacgtgaaa taccgttatg agtttacgga cacgctgggg ctgattacgt cattcagtta
     6481 tgccaacgct gaagatgagc aaaaaacgca ttacagcgat acccgctggc atgaggattc
     6541 cgtgcgtaac cgctggttca gcgtgatggc ggggccgtct gtgcgcgtga atgaatggtt
     6601 cagcgcgtat gcgatggcgg gcgtggctta cagccgtgtg tcgaccttct ccggggatta
     6661 tctccgcgta actgacaaca aggggaaaac gcacgatgtg ctgaccggaa gtgatggcgg
     6721 tcgccacagc aacacgtctc tggcgtgggg ggctggcgtg cagtttaacc cgaccgaatc
     6781 cgtggccatt gaccttgctt atgaaggttc cggcagtggt gactggcgca ctgacggttt
     6841 catcgtgggt gtcggttata agttctgatt agccaggtaa cacagtgtta tgactgcccg
     6901 ctggttcagg cgggcttttt gtggggtgaa tatggcagta aagatttcag gtgtactgaa
     6961 agatggggcg ggtaaacctg tcgtaaattg tgcgattgaa ctgcgggcca gaagaaccag
     7021 tccgaccgtt gtggcacacg ttgttgccac ttgcgtgacg gacaataacg gtgcttatgt
     7081 gattgaggct gagccggggt attacgaggt tgcgcttcac tgtaacggct ggcagccaac
     7141 ccgtgtcggg gatattgatg tggcaccgac tgatgcaccg gggacactga acgcgtttct
     7201 gaatgcacca aaggatggtg atttacgtcc ggaggtgat
//
LOCUS       DAJTZL010000186         7187 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.186, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000186 DAJTZL010000000
VERSION     DAJTZL010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7187
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.186"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..1143
                     /gene="gabD"
                     /locus_tag="OO544_003546"
     CDS             <1..1143
                     /gene="gabD"
                     /locus_tag="OO544_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HCR6769061.1"
                     /translation="AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQP
                     IGVTAAITPWNFPAAMITRKAGPALAAGCTMVLKPASQTPFSALALAELAIRAGIPAG
                     VFNVVTGSAGAVGNELTSNPLVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPF
                     IVFDDADLDKAVEGALASKFRNAGQTCVCANRLYVQDGVYDRFAEKLQQAVSKLHIGD
                     GLDKGVTIGPLIDEKAVAKVEEHIADALEKGARVVCGGKADERGGNFFQPTILVDVPA
                     NAKVSKEETFGPLAPLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYG
                     IVGINTGIISNEVAPFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            1157..2437
                     /gene="gabT"
                     /locus_tag="OO544_003547"
     CDS             1157..2437
                     /gene="gabT"
                     /locus_tag="OO544_003547"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HCR6769062.1"
                     /translation="MNSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGREYL
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGDFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYAAS
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEHENLLQKANDLGQKLKDG
                     LLAIAEKHPEIGDVRGLGAMIAIELFEDGDHSKPDAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIISQCFAEAKQ"
     gene            2748..4064
                     /locus_tag="OO544_003548"
     CDS             2748..4064
                     /locus_tag="OO544_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HCR6769063.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSA
                     LYTASRMLYSLSRRGDAPAVMGKINRSKTPYVAVLLSTGAAFLTVVVNYYAPAKVFKF
                     LIDSSGAIALLVYLVIAVSQLRMRKILRAEGSEIRLRMWLYPWLTWLVIGFITFVLVV
                     MLFRPAQQLEVISTGLLAIGIICTVPIMARWKKLVLWQKTPVHNTR"
     gene            4085..4747
                     /gene="csiR"
                     /locus_tag="OO544_003549"
     CDS             4085..4747
                     /gene="csiR"
                     /locus_tag="OO544_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HCR6769064.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(4748..5197)
                     /gene="kbp"
                     /locus_tag="OO544_003550"
     CDS             complement(4748..5197)
                     /gene="kbp"
                     /locus_tag="OO544_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HCR6769065.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(5281..5439)
                     /locus_tag="OO544_003551"
     CDS             complement(5281..5439)
                     /locus_tag="OO544_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HCR6769066.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            5622..5921
                     /locus_tag="OO544_003552"
     CDS             5622..5921
                     /locus_tag="OO544_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6769067.1"
                     /translation="MTELAQLQASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLIDSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            5931..6455
                     /gene="ygaP"
                     /locus_tag="OO544_003553"
     CDS             5931..6455
                     /gene="ygaP"
                     /locus_tag="OO544_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HCR6769068.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(6502..6906)
                     /gene="stpA"
                     /locus_tag="OO544_003554"
     CDS             complement(6502..6906)
                     /gene="stpA"
                     /locus_tag="OO544_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HCR6769069.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
BASE COUNT         1667 a         1792 c         1958 g         1770 t
ORIGIN      
        1 gccgaagcga aaggcgaaat cagctacgct gcctctttta ttgagtggtt tgccgaagaa
       61 ggcaaacgta tttatggcga taccattccc ggtcatcagg ccgataagcg cctgattgtt
      121 atcaagcagc cgattggcgt taccgcagct atcacgccgt ggaacttccc ggcggcgatg
      181 attacccgta aagccggtcc ggcgctggcg gcaggctgca ccatggtgct gaagcccgcc
      241 agtcagacac cgttctctgc gctggcgctg gcggagctgg cgattcgcgc gggcattccg
      301 gctggcgtgt ttaacgtagt caccggttcg gcaggcgcgg tcggtaatga actgaccagc
      361 aacccgctgg tacgcaaact gtcgtttacc ggttcgaccg aaattggccg ccagttaatg
      421 gaacagtgcg cgaaagacat caaaaaagtg tcgctggagc tgggcggcaa cgcgccgttt
      481 atcgtctttg acgatgccga tctcgataaa gccgtggaag gcgcgctggc ctcgaaattc
      541 cgcaacgccg ggcaaacctg cgtctgcgcc aaccgtttat acgtgcagga cggcgtgtat
      601 gaccgctttg ccgaaaaatt gcagcaggcg gtgagcaaac tgcacatcgg cgacgggctg
      661 gataaaggcg tcaccatcgg gccgctgatc gatgaaaaag cggtagcaaa agtggaagag
      721 catattgccg atgcgctgga gaaaggtgcg cgtgtggttt gcggcggtaa agcggatgaa
      781 cgcggcggca acttcttcca gccgaccatt ctggtggacg tgccggccaa cgccaaagtg
      841 tcgaaagaag agacgttcgg ccccctcgcc ccgctgttcc gctttaaaga tgaagccgat
      901 gtgattgcgc aagccaatga caccgagttt ggccttgccg cctatttcta cgcccgtgat
      961 ttaagccgcg tcttccgcgt gggcgaagcg ctggagtacg gcatcgtcgg catcaatacc
     1021 ggcattattt ccaatgaagt ggccccgttc ggcggcatca aagcctcggg tctgggtcgt
     1081 gaaggttcga agtatggcat cgaagattac ttagaaatca aatatatgtg catcggtctt
     1141 taactggaga atgcgaatga acagcaataa agagttaatg cagcgccgca gtcaggcgat
     1201 tccccgtggc gttgggcaaa ttcacccgat tttcgctgac cgcgcggaaa actgccgggt
     1261 gtgggacgtt gaaggccgtg agtatcttga tttcgcgggc gggattgcgg tgctcaatac
     1321 cgggcacctg catccgaagg tggtggccgc agtggaagcg cagttgaaaa aactgtcgca
     1381 cacctgcttc caggtgctgg cttacgagcc gtatctggag ctgtgcgaga ttatgaatca
     1441 gaaggtgccg ggcgatttcg ccaagaaaac gctgctggtt acgaccggtt ccgaagcggt
     1501 ggaaaacgcg gtaaaaatcg cccgcgccgc caccaaacgt agcggcacca tcgcttttag
     1561 cggcgcgtat cacgggcgca cgcattacac gctggcgctg accggcaagg tgaatccgta
     1621 ctctgcgggc atggggctga tgccgggtca tgtttatcgc gcgctttatc cttgcccgct
     1681 gcacggcata agcgaggatg acgctatcgc cagcatccac cggatcttca aaaatgatgc
     1741 cgcgccggaa gatatcgccg ccatcgtgat tgagccggtt cagggcgaag gcggtttcta
     1801 cgccgcgtcg ccagccttta tgcagcgttt acgcgctctg tgtgacgagc acgggatcat
     1861 gctgattgcc gatgaagtgc agagcggcgc ggggcgtacc ggcacgctgt ttgcgatgga
     1921 gcagatgggc gtggcgccgg atcttaccac ctttgcgaaa tcgatcgcag gcggtttccc
     1981 gctggcgggc gtcaccgggc gcgcggaagt gatggacgct gttgcaccgg gcggtctggg
     2041 cggcacctat gccggtaacc cgattgcctg cgtggctgcg ctggaagtgt tgaaggtgtt
     2101 cgagcatgaa aatctgctgc aaaaagccaa cgatctgggg cagaagctaa aagacggatt
     2161 gctggcgata gccgaaaaac acccggagat cggcgacgta cgcgggctgg gagcgatgat
     2221 tgccatcgag ctgtttgaag atggcgatca cagcaaaccg gacgcaaaac tcaccgccga
     2281 gatcgtggca cgcgcccgcg ataagggcct gattcttctc tcctgcggcc cgtattacaa
     2341 cgtgctgcgc atccttgtac cgctcaccat tgaagacgct cagatccgtc agggtctgga
     2401 gatcatcagc cagtgttttg ctgaggcgaa gcagtagcta tgtccgtttg taagagttaa
     2461 ccgttgctaa gggaatgata acgatgcagt aaaaaataat aacgatgagg cgaacgacaa
     2521 agttaaaaac gactgcggac agtcccctcg cccctctgga gagagggtta gggtgagggg
     2581 taaaacacgc ggctaaaccc tcccctggcc ctctccctaa gggagaggga gaaaaccctc
     2641 ccgctttcgt ttttaagatt ttgttgaccg tctcaattaa acgcgtgacc cggcgcggga
     2701 aatgtcgggg cgctctccca agtgacacac tttcgagagg attcaggatg gggcaatcat
     2761 cgcaaccaca tgagttaggc ggcgggctga aatcacgcca cgtcaccatg ttgtctattg
     2821 ccggtgttat cggcgcaagt ctgtttgtcg gttccagcgt cgccatcgcc gaagcgggcc
     2881 cggcggtatt actggcctat ctgttcgccg ggctactggt ggttatgatt atgcggatgt
     2941 tggcggaaat ggcggttgcc acgcccgata ccggttcgtt ttccacctat gccgataaag
     3001 ccattggtcg ctgggcgggc tataccatcg gctggttgta ctggtggttt tgggtactgg
     3061 ttatcccgct ggaagccaat atcgccgcta tgatcctgca ctcgtgggtt ccaggcattc
     3121 ccatctggtt attttccctc gtcattaccc ttgccttaac tggcagtaat ttattaagcg
     3181 ttaaaaacta cggcgaattt gagttctggc tggcgctgtg caaagtcatc gctatcctgg
     3241 cctttatttt ccttggtgca gtcgcaatta gcggttttta cccttatgcc gaagtgagcg
     3301 ggatctcaag attgtgggat agcggcggct ttatgcccaa cggtttcggt gcggtattaa
     3361 gcgcgatgtt gatcaccatg ttctcgttta tgggcgcaga aattgtcacc attgccgccg
     3421 cggaatccga cacgccggaa aaacatattg tccgcgccac caactcggtt atctggcgta
     3481 tttctatctt ctatttgtgc tctatttttg tcgtcgtggc attaataccg tggaatatgc
     3541 cggggctgaa agccgttgga tcttatcgct cggccctgta taccgcctca aggatgctct
     3601 actccttaag ccgtcgcggt gatgctcccg cggtaatggg taaaatcaac cgcagtaaaa
     3661 ccccgtacgt ggcggtgtta ctctccaccg gcgcggcatt cttaacggtg gtggtgaact
     3721 attacgcgcc tgcgaaggta tttaaatttc tgatcgacag ctccggcgct atcgccctgc
     3781 tggtttattt agtcatcgcc gtttcacagt tgcggatgcg caaaattctg cgagcggaag
     3841 gaagcgaaat tcgcttgcga atgtggcttt atccctggct cacctggctg gtcatcggct
     3901 ttattacctt tgtgttggta gtgatgctat tccgtccggc gcaacagtta gaagtgatct
     3961 ccaccggctt attagcgata gggattatct gtaccgtgcc aattatggct cgctggaaaa
     4021 agctggtatt gtggcaaaaa acacccgttc ataatacgcg ctgataatga tcaggagtca
     4081 caccatgacc attacgtctc tggatggcta tcgctggctg aagaacgata ttattcgcgg
     4141 taattttcaa ccggatgaaa aattacggat gagtttgctg acatcgcgtt atgcacttgg
     4201 cgttgggccg ttgcgggaag cactttctca actggtggcg gaacggctgg tcacggtggt
     4261 gaatcaaaaa gggtatcggg tggcgtctat gtcagagcag gagctgctcg atattttcga
     4321 tgcccgcgcc aatatggaag cgatgttagt gagtctggcg attgcccgcg gtggcgatga
     4381 gtgggaggca gacgttctcg caaaagcgca tctgctcagt aagctggaag cctgtgacgc
     4441 cagcgagaaa atgcttgatg agtgggatct gcgtcatcag gcgtttcata cagcaattgt
     4501 ggcgggctgt ggttctcact atttgctgca aatgcgtgaa cggttgtttg atctggcggc
     4561 gcgctatcga tttatctggc tgcggcgaac ggtgctttcg gtagaaatgc tggaggataa
     4621 acacgatcag caccagaccc tgactgcggc ggtactggcg cgagataccg cgcgcgccag
     4681 tgagttaatg cgccagcatt tactgacgcc aattcccatt atccagcagg cgatggctgg
     4741 caattaacta ctcttccgga atacgcaaca cttgccccgg ataaatttta tccgggcttt
     4801 ttagcatcgg tttattcgct tcgaagattt tattgtacag attagcgtta ccgtagacct
     4861 gtttggaaat ggcactcaga gtgtcgccag acttaacggt ataaaactgg ctggcagtgg
     4921 ctggtgtcgc cgttttcacc tgatcatcaa cactggcaat accggaaata ttccccaccg
     4981 caacaaggat tttctccttc gcctcctgac tcaggccgtc accagtgacc gtcgctttgc
     5041 cgtcggcaat ttgaatgttc actttatcag catcaggtat accggttttg ctcagatgct
     5101 cctgcacctt cttcgcctga tcgtctttat cgtgctgacc tgtaaccgcg tcccagagtt
     5161 tttctccggc atctttcaca aaattgaaca gacccatagc tacctcattc gttaacggaa
     5221 acactcagaa agtgtagcag aggaacgcgg cgcgttaacg ccgcgggggt gagcacaggt
     5281 ttaatcgcgg gtttgcaccc agaacgcgtg aatcaaaccg gggatatacc caagcagtgt
     5341 cagcagaata ttaataatga acgcccaacc gaacccttta ccgagcagca cgccgagcgg
     5401 cggtagaatg atggtgatga cgattctcca gaaacccata tgtactccct ataagaaaat
     5461 tactcattgt ttaaaaagag attttatctc ttaagcgtag taattttagc ggagcctgcc
     5521 agtttcagct tacgctttta ctctcattta cgctgctgag gctggtatat gttgcatttt
     5581 tagtttagag cttacttaat ttagaaaata cttaaataaa tatgactgaa ctcgcgcaat
     5641 tacaggccag tgccgaacag gcagcggcct tattgaaagc aatgagccat cctaaacgat
     5701 tgctgattct gtgcatgctt agcggttccc ccggcaccag cgcgggagag ctgacgcgca
     5761 ttaccggact gagtgcctct gcgacatcac agcatctcgc tcgtatgcgg gacgaagggc
     5821 ttatcgacag ccaacgggat gcccaacgca ttctatattc cattaaaaat gaggcggtaa
     5881 atgccattat cgccaccctg aaaaatgtct attgtccgta aggagtcact atggctttga
     5941 caaccatttc gccgcatgat gcacaagaat taatcgcacg cggcgcaaag ttaatcgata
     6001 ttcgtgacgc tgatgaatat cttcgtgaac atattcctga agcagatctg gctccattat
     6061 ccgtgctgga acagtcaggt cttccaccta aattacgtcg cgagcaaatt atattccact
     6121 gccaggcagg taaacgcacc agtaataacg ccgataaatt agcagcgatt gccgcccccg
     6181 cagaaatatt tttactcgaa gatgggattg atggctggaa aagagctgga ttgccagtag
     6241 cggtaaataa atctcaaccc ttgccgttaa tgcgccaggt gcagatcgct gcgggtggtt
     6301 taatattaat cggcgttgta ctgggttata ccgtaaatag cggtttcttc ttattaagtg
     6361 gctttgttgg tgccgggtta ctgtttgcag gaatcagcgg tttttgtgga atggcaaggt
     6421 tgttagataa gatgccgtgg aaccaacgag cttgagaagc gacgccggac gcgccctagc
     6481 agcgacatcc ggcctcagta attagatcag gaaatcgtcg agagatttac cttctgccag
     6541 cgcctgagca attggcttcg gtgtacggcc ctgaccggtc caggttttag tttcaccgtt
     6601 aacatcggtg aatttatatt tcgccggacg cggctggcgt tttttaccag cgcgtggtgc
     6661 agcagcagag ctattaccca ataactcttc cgggttaatt ccgtcagcct tcatcagctc
     6721 cagccaggtg ctaatttttt cctgacgctc agccagttca cgctgctgct gttcttcttc
     6781 ttcacgtctt tctttagtga caaccctgaa tttttcgagc atttcttcaa gaacgtcaat
     6841 ggagaattcg cgagccatcg cacggagggt gcgaatgtta tttaaacttt gtaacattac
     6901 ggacataata aagaaaacct tttaacgcca aaacaaaaaa gtatttcgtc gcgtagtgta
     6961 agcgatcact tattatattt ccacaaaatt taatctattt acagtcttgc gcgagattat
     7021 ttcagctgtt attttctgaa actctcagaa taatttcaag attatatatt agccattttg
     7081 atgagtaatt tagattattg gtgaacagaa cggcgctctc tgctcgctaa ttccgcaagc
     7141 ctgacaaaac cgaagtgaat aaaaatgcaa tataacagaa taaaaga
//
LOCUS       DAJTZL010000187         7121 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.187, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000187 DAJTZL010000000
VERSION     DAJTZL010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7121
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.187"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            4..435
                     /locus_tag="OO544_003555"
     CDS             4..435
                     /locus_tag="OO544_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HCR6769070.1"
                     /translation="MALNLASGEGNFFIRPGGVFYVAGDKVDIVRLDAFKTSKEIQFA
                     VQSGPMLMENSVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKA
                     KLNVEQLLYLDGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            518..1420
                     /locus_tag="OO544_003556"
     CDS             518..1420
                     /locus_tag="OO544_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HCR6769071.1"
                     /translation="MKKTLATLFLLTCLGSSSAYADNALILQTDFSLKDGAVSAMKGV
                     AFGVDHNLKIFDLTHEIPPYNIWEGAYRLYQTASYWPQGSVFVSVVDPGVGTDRKSVV
                     LKTKNGQYFVSPDNGTLTLVAESLGIESVRQIDEKTNRLKGSEKSYTFHGRDVYAYTG
                     ARLASGAITFEQVGPELPAKVVELSYQKAKATKGEVKGNIPILDIQYGNVWSNISDEL
                     LNQAGIKLNDTLCVTISEGSQQKYAGKMPYVASFGDVPEGQPMVYLNSLLNVSVALNM
                     DNFAQKHQVASGADWNIDVKKCAK"
     gene            complement(1475..3637)
                     /gene="uvrD"
                     /locus_tag="OO544_003557"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(1475..3637)
                     /gene="uvrD"
                     /locus_tag="OO544_003557"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HCR6769072.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTLHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLETV"
     gene            complement(3721..4437)
                     /gene="yigB"
                     /locus_tag="OO544_003558"
     CDS             complement(3721..4437)
                     /gene="yigB"
                     /locus_tag="OO544_003558"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HCR6769073.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(4437..5333)
                     /gene="xerC"
                     /locus_tag="OO544_003559"
     CDS             complement(4437..5333)
                     /gene="xerC"
                     /locus_tag="OO544_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HCR6769074.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDVTMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(5330..6037)
                     /locus_tag="OO544_003560"
     CDS             complement(5330..6037)
                     /locus_tag="OO544_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HCR6769075.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(6034..6858)
                     /gene="dapF"
                     /locus_tag="OO544_003561"
     CDS             complement(6034..6858)
                     /gene="dapF"
                     /locus_tag="OO544_003561"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HCR6769076.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(6895..7098)
                     /locus_tag="OO544_003562"
     CDS             complement(6895..7098)
                     /locus_tag="OO544_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR6769077.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
BASE COUNT         1640 a         1991 c         1853 g         1637 t
ORIGIN      
        1 aaggtggcgt taaatctcgc ttcaggtgaa gggaatttct ttatccgtcc tggcggcgtg
       61 ttttatgtcg cgggagataa agtcgacatc gttcgtctgg atgccttcaa aaccagtaaa
      121 gagattcagt ttgcggtgca gtcagggcca atgttgatgg aaaacagtgt aattaatccg
      181 cgtattcatc ccaacgtcgc ctcacgtaaa attcgtaacg gtgttgggat taataaacat
      241 gggaatgccg tgtttttgtt gagccagcag gcgacaaatt tttatgattt tgcctgttat
      301 gccaaagcga aactgaacgt tgagcagctg ctttatcttg atggcacgat ttcacatatg
      361 tatatgaaag gcggggcaat cccgtggcaa cgctatcctt ttgtcaccat gatttccgtc
      421 gagaggaaag gttaagcatt gtacactcga ggaggccaga caaactgggg ctggctacac
      481 agctgaaaaa tcatcagata aatgaaggga aacaaaaatg aaaaaaacgc tcgcaacgtt
      541 atttttatta acgtgtctgg gatcatcatc cgcttatgcg gataacgctt tgattttaca
      601 aactgatttt agcctgaaag atggtgccgt ctcggcgatg aaaggggtcg cattcggtgt
      661 tgaccataat cttaaaatat ttgatttaac acacgaaatt ccaccgtata acatctggga
      721 aggcgcttac cgcctgtatc agaccgccag ttactggccg caaggttcgg tatttgtcag
      781 tgtggtcgat cctggtgttg gtacagaccg taaatcggta gtgctaaaaa ccaaaaacgg
      841 tcaatacttc gtttcaccag ataacggtac gctcactttg gtggcggaat cgctgggtat
      901 tgagagcgtc cggcaaatcg atgaaaaaac caaccgcctg aaaggttccg aaaagtcata
      961 taccttccac ggtcgtgacg tgtatgccta caccggtgca cgcctggctt ccggagcgat
     1021 cacgttcgaa caggtggggc cagagcttcc tgcaaaagtt gtcgaacttt cttatcagaa
     1081 agccaaagca acaaaaggtg aagtgaaggg caatatcccg attctcgata ttcagtacgg
     1141 caacgtctgg agcaatatca gcgacgagtt gctgaatcag gcaggaatca aactcaacga
     1201 cacgttgtgt gtaaccattt ccgaaggttc ccagcaaaaa tacgcaggga aaatgccgta
     1261 tgtcgcaagc ttcggcgatg tgccggaagg ccagccgatg gtgtatctaa acagcttatt
     1321 gaatgtttcc gtggcgctca atatggataa tttcgcgcag aaacatcaag ttgcgtctgg
     1381 tgctgactgg aatattgatg tgaagaagtg cgctaagtaa ggtgctgatg caggatgcgg
     1441 tgtggcgcac cgcatcctgc aacggagttg taaattacac cgtctccagc cgggcgtaag
     1501 ccgccaccag ccatttgatt ccctggccct ggaatgccac ctgcaaacgg ctatgttcac
     1561 cgctgccttc catattgacg atggtgcctt caccaaactt agcgtggcgc acgcgctggc
     1621 cgagtttgta gccgctgtcg ttctcgacca tcggcgtacc catccgctga tggctaaccg
     1681 ggcggcttac cgtggcgcgc aggcgcacct cttccacaca ctcttccggc agctcgccga
     1741 taaagcgcga cgggcgatgg taaacctctt tgccatacag acggcgagtt tccgcgtagg
     1801 tcagcgtcag tttctgcatc gcacgggtta cgccaacgta ggccagacgg cgttcttctt
     1861 ccagacgtcc gccttcatcc agcgacatct ggcttgggaa catgccctct tccataccga
     1921 cgataaacac ctgcgggaac tccagccctt tcgccgagtg tagcgtcatc aactgcaccg
     1981 catcctgcca agtatccgcc tgcccttcac ctgcttccag tgccgcatgg gagaggaacg
     2041 cctgcagcgg cattaaatct tcgtcttctt cgttgtagct gaactggcgc gttgccgtca
     2101 ccagttcctc taagttttcg atacgcgtct gacctttttc gcccttctcc tgctcgtaca
     2161 tggtacgcag gccggagtct ttaattaccc ggtcagtctg tacatgcagc ggcatatcgg
     2221 cagtttcttg cgctaaggca tcgattaatt ccataaatcg ctgcaaggcg ctggcagctc
     2281 gcccggcgag ggctttttcc tgcaacagtt cacgacatgc ctgccagagt gttaactggc
     2341 gatcgcgcga tgtctgacgt accacgtcca gcgtccggtc accaataccc cgcgttggcg
     2401 tattcaccac gcgctcaaac gccgcgtcgt cgttacggtt ggcaatcaga cgcagatacg
     2461 agagcgcgtc tttgatttcc tggcgttcga agaagcgcat cccgccgtaa atacggtacg
     2521 gcatactggc ctgcaacagt gcttcttcca gtacacgcga ctgggcgtta ctgcggtaga
     2581 gaatggcgca ctcggcaagc gccccgccgt tgtcctgcca agttttgatg cggttaacca
     2641 caaaacgcgc ttcgtcgagt tcgttaaaag cgcaatagag ggaaataggc tcaccgtccg
     2701 cgccatcggt ccacagtttt ttacccagac gcccgttgtt gttttcaatc agggcgttcg
     2761 cggcgctcag aatattgctg gtagagcggt agttctgctc cagacggata gtttcagcac
     2821 cggggaaatc attgaggaaa cgctgaatat tctctacctg cgccccgcgc cagccgtaga
     2881 ttgactggtc gtcatcgcca acgatcatca ctttgccggt atcgcccgcc agcaggcgga
     2941 tccacgcgta ctgaatgttg ttggtatcct ggaattcgtc caccaggatg ttggtaaaac
     3001 gttcgcggta gtgttgcagg atatgcggct tgttaagcca caactcgtga gcgcgcagca
     3061 gcagctcggc gaagtccacc aggcccgcac gatcgcacgc ttcctgatac gcctgataca
     3121 ccttctgcca ggtctgctcc accggattac cgtagctttg aatatgatgc ggacgcaggc
     3181 cttcatcttt ctggctgttg atgtaccaca ttgcctggcg cggcggccac tgcttctcgt
     3241 cgaggttcat ggctttgatc agacgcttaa gcaggcgtag ctggtcttca ctgtcgagga
     3301 tctggaaatc ctgcggtaga ttggcgtcca tatggtgcgc acgcagcaaa cggtgcgcca
     3361 gcccgtggaa ggtgccgacc cacatgccgc cctggctggt gcccatcagt tgcccgatac
     3421 gatgacgcat ctccgccgcc gctttgttgg taaacgtcac agccataatc gagtatggcg
     3481 agcagttttc cacgctcatc agccaggcga tacgatgcac cagtacgcgc gtcttaccac
     3541 tgcccgcgcc cgccagcacc agaaggttgc tgcgtggcgc ggccaccgct tcgcgctgtt
     3601 tgtcattaag gctgtcgagc aggtaagaaa cgtccattgg caccgccgcg taaaaatagg
     3661 ttgggcggag aagcgcaacg ccctccgcca aaaagctggg tatatataca gatttactga
     3721 ttatatcagc gaggtcagag atgccaaccg ggaaatttcc agatgcggca gtaaacggct
     3781 gtcccaggtt tgcatcagat cgccattttc cggtctgatc caacaagcct gcattccgct
     3841 gcgaattgcc ccacccacgt cagtggtgag atcgtcccca acatgtaaga tctcgccgat
     3901 cggcacgttg agtttttccg cagccaaaaa gtacatatcg ctgaacggtt ttgagcgccc
     3961 atgcgggcca gcgcgcagca caaactcaaa ataatccccc aaaccaaaca gttccggctg
     4021 ggcgttaccg ttggtgatcg ccaccagcgg ccatttcttc gccagctgtt ttaaggtgtc
     4081 gtgagtttgc tgcgggacgt cgattcggct gcgccatttg gcaaagttaa tcattgctgc
     4141 gtgtgcgcct gcactggctt cttcggcact cagcccggcg tcgagcatcg cttgttcaat
     4201 cgaacgaaaa cgccaacgcg tcacgtcgtg ataaatctcg ggttccgctt cccgtaccgc
     4261 ctggcgcagg cgttgcagat cttcattctg gaagctgcgc agcgccggat gataattttg
     4321 cacaaaggta agcgcctctc gctcggtgcg caaaatcacc ggacggttat cgtaaagggt
     4381 atcatccagg tcaaaggtga gcgccgagat gcgccccaaa ggccggtaaa aacgcattat
     4441 ttcccccgtt tggcgcgtgg atgcgccgca tcgtacaccg aggcaaggtg ttgaaaatca
     4501 agatgagtat agatttgcgt ggtggagagg ttggcatgac ccagcagctc ctgcacacca
     4561 cgaagatcgc cgctcgactc cagcatatgc gtggcgaacg agtgacgtaa tttatgcgga
     4621 tgaacgtgat tattcagccc ttgttttatg ccccattcgg caaagcgttt ctgcacatta
     4681 cgcgcggaga tacgcttgcc cagtttcgac agaaaaagcg cgtcgtcttc gctaccaaac
     4741 aggtcgcgca aatcaagcca gtgctcaatc cacgccacag cgttgcgacc aatcggcagg
     4801 cggcgctctt tgctgccttt ccccattacc cacacttcac cagactccag gtcgaggtgt
     4861 ttgatatcca gccccaccag ctcggaaaga cgcagacccg cgccgtacat cacttccagc
     4921 attgcacggt cgcgtacagc gaggggatcg ttgatatcaa tgtccagcag ccgattcata
     4981 tcgtcgacgt cgatattttt cggcagatga cgcggcgctt tcggtgccga aacacctttc
     5041 gccgggttag ctttgagttc gttctggctg accagccagt caaaaaagct acgtagcgca
     5101 gaaagccgta acgcgaggct tgctgctccc agccctttac ggcgactgcg tacagcaaaa
     5161 ttgcgcacca tcgtcacatc acattgctgc cagctttgca ggccgttttc gctggcaaaa
     5221 ttgatgatcg cctcaagctg acgctggtag ttaagcaggg ttatcgggct aagctggcgc
     5281 tccacgctca gataacgtag gtagcgttct acatcggtgt gtaaatcggt catacgcgtt
     5341 caatccaacg ctccagaagc tccggcaaca tcagcgcaat ttcatgaagt aactgcgttc
     5401 cctgcccttg ttgatagtga ctggcatcgc gactggtaaa cagcacgaca cccaaatcag
     5461 catcgcttcc cagcatcgac atcgccaccg atcccaccgc tttcgcttcc ggtagcacca
     5521 ccagcagctc tggtccgtta agcggcccaa gatagtgctg ttcctgcccc aaacgctgaa
     5581 tacgcagcgg ttcgaaagac tgacggctta atgccagatg agtgtggttc gacggcgcac
     5641 ctaagcgcca gcgatccgga aacaggcgca gactcgcacc tgccaggccg agatcgcgcg
     5701 cccagcggtg aaagcgcatc agcatatcgt cgagactgct ggcggcggtg agactgcgct
     5761 gcaggtagag taggcgataa aacaggcctt cgttggcgat agcctgttcc atcaacagcg
     5821 ccatgttctc ttccagaaca tgaatatgat tacgtgcgcg ggccatgtgc cactcgacca
     5881 acgaaacggt gccgcgtacc ggatgcggca cacgtatcgc ttctactgcg cgcgcattac
     5941 ggataaaaaa ctcaggattt ttaatcagat aatcgacaac cgcccggtca tcaagctccg
     6001 tgagtgtttc ctgcagttct tcccctggtt gcttcataga tgaataaatc cgtcgtagac
     6061 atgtaccgcc gggccagtca tatataacgg gtgacccgga cctttccagg cgatatcaag
     6121 acgaccgccg gggagttcca cgcgtacttc ttcggccagc aaaccttgct gaatccctac
     6181 tgcaaccgcc gcacacgcgc cgctgccgca ggcctgggtt tctcctgccc cacgctcata
     6241 aacgcgtaaa cgaatatgct cgcgcttaac cacttgcata aaaccgatat tggcgcgctc
     6301 cggaaaacgc tcgtggcttt ccagaacagg accaagcgtt tctaccgccg cggtatcgac
     6361 atcatcgacc tgaatcacgc aatgcggatt tcccatcgac accacgccgc ataagattgt
     6421 ctgctcggcg gcgcgcataa tataggtctt ttccgctttg ttagcgcgaa acggcacggc
     6481 ggaaggttcg aagttgggtt cgcccatatt tacgcggacc agatcatcat cggtgacggt
     6541 cagaaccatc cgcccgttgg cggtgctgac gcggatatca cgcttattgg tcagtccttt
     6601 cagacgcaca aaacgggcaa agcagcgcgc accgttgccg cactgcgcca cttcactgcc
     6661 atcagcattg aaaatgcgat agtgaaaatc cagttcagga tcatacggcg gctcaaccac
     6721 cagcagttgg tcaaacccta cccccaggtg ccgatcagcc aggcgacgaa tcagctccgg
     6781 tgaaaaaaag acattctgcg ttaccgcgtc gacgaccata aaatcgttgc caaggccatg
     6841 cattttcgag aactgcatca tttactccaa tcacgcgggt acagaaactg acttttagta
     6901 attcacctgg gatggaccat cgccagtggc gcgatcgttt ttatccggca ccgtggattg
     6961 cgtttgcgtc tctaccggtt tggtcggcgg cggtgcgttt ttatctgcag gcgggaaata
     7021 gagcggacct ttcagaccgc agcccgtcag gctgaagaga gtaagtaata cagtgagtgc
     7081 cttaaacacg tttttcatta tggattgcct gtaaagttca t
//
LOCUS       DAJTZL010000188         7007 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.189, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000188 DAJTZL010000000
VERSION     DAJTZL010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7007
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.189"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            689..1459
                     /locus_tag="OO544_003563"
     CDS             689..1459
                     /locus_tag="OO544_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HCR6769078.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNKGGWDGAVITFFASTAAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1450..1923
                     /locus_tag="OO544_003564"
     CDS             1450..1923
                     /locus_tag="OO544_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HCR6769079.1"
                     /translation="MGVIEFLFALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGAI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            2031..2570
                     /gene="dnaT"
                     /locus_tag="OO544_003565"
     CDS             2031..2570
                     /gene="dnaT"
                     /locus_tag="OO544_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HCR6769080.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2573..3310
                     /gene="dnaC"
                     /locus_tag="OO544_003566"
     CDS             2573..3310
                     /gene="dnaC"
                     /locus_tag="OO544_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HCR6769081.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            3359..3853
                     /locus_tag="OO544_003567"
     CDS             3359..3853
                     /locus_tag="OO544_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HCR6769082.1"
                     /translation="MMKTVKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            4107..6398
                     /gene="opgB"
                     /locus_tag="OO544_003568"
     CDS             4107..6398
                     /gene="opgB"
                     /locus_tag="OO544_003568"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HCR6769083.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIAAFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWQGKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6537..>7007)
                     /gene="lgoD"
                     /locus_tag="OO544_003569"
     CDS             complement(6537..>7007)
                     /gene="lgoD"
                     /locus_tag="OO544_003569"
                     /EC_number="1.1.1.414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HCR6769084.1"
                     /translation="DGAQVVVADTSPARREHVATRLELPLLDPSAEDFDAQLRAQFGG
                     SLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHKKETTMMGSRN
                     ATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELIKGVITF"
BASE COUNT         1776 a         1798 c         1719 g         1714 t
ORIGIN      
        1 cttttaaccc tgcttgcata acaggtattt tactgataac aattccattt ttgcagcatc
       61 ctggcaacat atttcctcca catccactgt ctaaaaatca tgatggttta ttcaggatat
      121 tcattcctta aatgtttcca gcaaattaat agattaagcg aataaattat ccttagcatt
      181 cactttcata tcagcatact cctgctgcac aacaactcca ctgcatgtgt cagaaggtta
      241 gcatcaaatg ataacctggt gaaatagcct ggagacattt aaaatatcta cattaagttg
      301 ttaggaatac ttaatgaaaa acagaaatca cattcattta atttataatt aaatgatatc
      361 ccttcccgca gaatgaaaaa tcacacaacc aatatttatt caatgaaatc tgatggtttt
      421 aacttgttaa cattaaaaaa cgtgcccggt tatctattat cctgattcgt aaaggccaaa
      481 caataaattc attatagtga taatattcat gtgatagttt gtttgcggcg agagataatt
      541 cgctttttat caccgagctt gtcccgataa ccaggattaa tcagcgcaat tttgatctta
      601 acctaccgca aaacaaccgt gcttcatgat acaaatggat tctcattatc tgaacaatac
      661 gcagcacgtc tatgacaaag ggcgagttat gcaaactgag caacagcgag ccgtaacacg
      721 gctttgtatc cagtgtggat tatttctttt gcaacatggt gcggaaagcg cgttggttga
      781 tgagctttcc tcacgactgg gtcgggcgtt ggggatggac agcgtcgaaa gttctatctc
      841 ttcgaacgcc atagtgctga caaccattaa agatgggcaa tgcctgacat cgacacgtaa
      901 aaatcacgat cgcggcatta atatgcatgt ggtgactgaa gtccagcaca ttgtgattct
      961 ggcggagcac catctgctgg attacaaagg cgtagagaaa cgatttagcc aaattcagcc
     1021 attacgttac ccaagatggc tggtagcctt aatggttggc ctttcttgcg cctgtttctg
     1081 caaactcaat aaaggtggct gggatggtgc cgtcatcacc ttctttgcca gtacggccgc
     1141 gatgtatatc cgccagctgc tggcacaacg tcatcttcat ccgcagatca acttttgcct
     1201 caccgctttc gccgccacca ccatttccgg attgcttttg caacttccca ctttcagcaa
     1261 tacacccact atcgcgatgg ccgccagcgt tctgctgctg gtgccgggat ttccgttgat
     1321 taatgccgtc gccgatatgt ttaaaggcca catcaatacc ggactggcac gctgggcgat
     1381 cgccagtctg ctgacactgg ctacctgcgt cggcgtggtg atggcactga cgatttgggg
     1441 gctacgcgga tgggtgtgat cgagtttctg tttgcattgg cgcaggatat gatcctcgcc
     1501 gccattccag cggtcggctt tgcgatggtg ttcaacgttc ccgttcgggc attacgctgg
     1561 tgtgcgctgc ttggcgcgat tggtcatggt tcacgaatga tcttaatgac cagcgggctg
     1621 aatattgagt ggtcaacctt tatggcctcc atgctggtcg gcaccattgg tattcaatgg
     1681 tcgcgctggt atctggcgca tccgaaagtg tttaccgtgg cggccgttat ccctatgttc
     1741 ccgggcatat cggcttatac cgcaatgatt tcggcggtaa aaatcagcca gttaggttac
     1801 agcgaaccgt tgatgattac cctgttaacc aactttctta cagcttcatc gattgttggt
     1861 gcgttatcca tcggtctttc cattcctgga ttatggttgt accgcaagcg ccctcgcgta
     1921 taaaattgcc tcgcttaaag cacacggatg agagacagcc tcctctcctc cgtgtgttac
     1981 tataaaagtt atctcccttc tcgttcatcg ttccatattt gagaaacagt atgtcttcca
     2041 gagttttgac cccggacgtc gttggtattg acgccctggt acacgatcac caaaccgttc
     2101 tggcaaaagc tgaaggcggt gtggttgccg tatttgctaa caatgccccg gcgttttatg
     2161 ccgtcacgcc tgcacgcctg gctgaactgc tggcgctgga agaaaagctg gcgcgtccgg
     2221 gaagcgatgt cgctctggac gatcaactct atcaggaacc gcaagccgct cccgttgctg
     2281 tacccatggg gaaattcgcc atgtatccgg actggcaacc cgatgccgat tttatccgcc
     2341 tggcggcgct atggggtgtg gcactaagag agccggtgac caccgaagaa ctggcctcat
     2401 tcattgccta ctggcaggcg gaaggtaaag tctttcacca tgtgcagtgg caacaaaaac
     2461 tggcgcgcag cctgcaaatc ggtcgtgcca gcaacggcgg actgccgaaa cgagatgtga
     2521 atacggtcag cgaacctgac agtcaaattc caccaggatt cagagggtaa cgatgaaaaa
     2581 cgttggcgac ctgatgcaac gcctgcaaaa aatgatgcct gcccatatca aacctgcatt
     2641 caaaacgggt gaagaactac tggcctggca gaaagaacaa ggagcgatcc gctccgccgc
     2701 tctcgaacgt gaaaatcggg cgatgaaaat gcagcgcacc tttaaccgct ccggtattcg
     2761 gccactgcat cagaactgct cctttgagaa ctatcgcgtt gagtgtgaag ggcagatgaa
     2821 tgcgttaagc aaagcgcgcc agtacgtcga agagtttgac ggcaacatcg ccagcttcat
     2881 cttttctggt aagccaggaa ccggcaaaaa ccatctggcg gcggcaatct gcaacgagct
     2941 gctgctacgc ggtaaatccg tattgatcat caccgtggcc gatattatgt cggcgatgaa
     3001 agataccttc aggaatagcg gtaccagtga agaacaactg cttaacgatc tgagcaacgt
     3061 tgatctgctg gtgatcgatg agatcggcgt gcagaccgaa tcgaaatacg aaaaagtgat
     3121 catcaaccag atcgtcgatc gccgctcttc ttccaaacgc ccaaccggga tgctgaccaa
     3181 cagtaatatg gaagagatga ccaagttgct gggcgaacgc gtgatggacc gtatgcgcct
     3241 gggtaacagt ttgtgggtga tcttcaactg ggatagctac cgtagccggg taacaggtaa
     3301 agagtattaa tccccgtttc tggcggtata ctgcgacaca attccacctt gagtaatgat
     3361 gatgaaaact gttaaacatc ttctgtgctg tgccattgcc gccagcgcat taatttccac
     3421 cggggtgcat gctgcgtcct ggaaagatgc gctctccagc gccgccagcg aacttggcaa
     3481 ccaaaacagc acgacacagg aaggcggttg gtcgctcgcg tcattaacta acttgcttag
     3541 cagcgggaac caggccttaa gcgcagataa catgaacaac gccgcaggca ttctgcaata
     3601 ctgcgcgaag caaaagctgg cgtcggtaac cgatgccgaa aacatcaaga accaggtgct
     3661 ggaaaagctg ggcctgaaca gtgaagagca aaaagaagac accaactatc tggatggcat
     3721 tcagggtttg ctgaaaacca aagatggtca gcaactcaat ctggataaca tcggaacgac
     3781 tccgctggca gaaaaggtga aaaccaaagc ctgcgatctg gtgttaaagc aggggctgaa
     3841 cttcatttcc tgaaccgcga aacgccgcgt tttagctgtt acccggctga cgcggctctc
     3901 tcctgccctt ttccctgctc ctggacggtt ttccccctgt tacacgggaa gcgaccgcga
     3961 tcaaaaaagt ctgtttataa actaattcct aacaacatcg catttttttg acactagaat
     4021 tgcagcaata tggcgacgcc attacattgt gcttctcaaa aagcttaaac gttggctaaa
     4081 cggcccggtt tagtgaggat cgcccgttgt cagaactact ctctttcgcc ctttttctcg
     4141 cctctgtgct gatttacgca tggaaagcgg gacgtaacac ctggtggttt gcagccacat
     4201 taacggtgct ggggctattt gtcgttttaa atatcaccct gtttgccagc gactatttta
     4261 ctggcgatgg tattaacgac gcggttctct ataccttaac caacagcctg accggtgctg
     4321 gcgtcagcaa atacatcctg ccgggtatcg gcattgtgct ggggctgaca gcggtgttcg
     4381 gtgcgctggg ctggatcctg cgccgtcgtc gccatcatcc gcaccatttt ggttacagcc
     4441 tgctggcgct cttactggcg ctgggttcgg tggacgccag cccggcattt cgtcagataa
     4501 cggaactggt gaaatcccag tcacgcgatg gcgacccgga ctttgcggct tattataaag
     4561 agccgtcgaa aactatccct gacccgaaac tcaacctggt ttatatctac ggcgaaagtc
     4621 tcgagcggac ctattttgat aacgaagctt tcccggatct cacgcctgaa ctgggcgcgt
     4681 tgaaaaatga aggcctggat ttcagccaca cgcagcagct gccaggaacg gattacacga
     4741 ttgcgggcat ggtggcttct cagtgcggca taccgctgtt tgcccccttt gaaggcaacg
     4801 cctccgcctc tgtctccagc ttcttcccgc agaacatctg tctgggcgat atcctgaaaa
     4861 actcgggtta tcagaactat ttcgtgcagg gcgcgaatct gcgttttgcc ggtaaagatg
     4921 tgttcctgaa gtcgcatggc ttcgaccact tatacggctc agaagagctg aaaagcgtgg
     4981 tggccgaccc gcactatcgc aacgactggg gattctacga cgataccgtt ctcgatgaag
     5041 cgtggaaaaa gtttgaagag ctttcccgct caggtcagcg attctcactg tttaccctga
     5101 cagtcgatac ccatcacccg gatggtttta tctcgcgtac ctgtaaccgc aaaaaatatg
     5161 attttgacgg taagccgaac cagtcattca gcgcggtaag ttgcagccag gagaatatcg
     5221 cggcgtttat caacaaaatc aaagcgtcac cgtggtttaa agataccgtc atcgtcgtct
     5281 cttctgacca tttagcgatg aacaacacgg cgtggaaata cctcaataag caggatcgca
     5341 ataacctgtt ttttgtcatt cgtggcgaca agccgcagca agagacgctg gcagtgaagc
     5401 gtaacacgat ggataacggc gcgacggtgc tggacattct cggtggcgat aactatctcg
     5461 gacttggtcg tagcagttta tccgggcagt cgatgtcgga aatcttcctc aatatcaaag
     5521 agaaaacatt ggcgtggaag ccggatatca tccgcctgtg gaaattccct aaagagatga
     5581 aagagttcac catcgaccag cagaaaaaca tgattgcctt ctcgggtagc catttccgtt
     5641 tgccgctgct gttgcgggtt tcagacaaac gcgtggaacc gctgccggaa agcgaatact
     5701 cagcaccgct gcgtttccag ctggccgatt tcgctccacg cgacaatttc gtctgggttg
     5761 accgttgcta taagatggca caactctggg ctccggaact ggcactctcc accgactggt
     5821 gtgtctcgca agggcagctt ggcggtcagc aaattgttca gcatgttgac aaaacaacat
     5881 ggcagggcaa aacggcattt aaagatacgg tcatcgacat ggcgcgttat aaaggcaatg
     5941 tcgatacgct gaaaattgtt gataacgata ttcgctacaa agccgacagt ttcatcttca
     6001 acgtcgccgg tgcgccagaa gaggtgaaac agtttagtgg gatttcccgt ccagagtcgt
     6061 ggggccgctg gtccaacgcg cagctgggcg atgaagtaaa aatcgagtac aagcatccgc
     6121 tgccaaagaa atttgacctg gtgattaccg ccaaagcata cggcaataac gccagccgtc
     6181 ctattccggt acgcgtaggc aatgaagaac aaacccttgt gctgggcaat gaagtgacca
     6241 ccaccacact gcatttcgat aacccaaccg atgccgacac actggtaatt gtgccgccgg
     6301 aacctgtctc aaccaacgaa gggaatatcc tcggacactc gccgcgtaag ctcgggatcg
     6361 gcatggtgga aattaaagtg gtggaacgtg aaggataatt cctgactgat gtgtaggtcg
     6421 cataaggtgc gacctacaca ccttctgcgg cctacaaaac atcattgaga aacagaaaaa
     6481 aacagcgccc aacacttccc cggacgctgc atggcaactt taataatctc gccttatcag
     6541 aaagtaatta cacccttaat caactcacga ttgttaatca catcgcgctc gtaggtttct
     6601 gccagcgtgg cgaacggata gcgatgggtt aacatcatgt cagcagtgat ttttccttcc
     6661 gccatcagtc gaccgacttt agcaaaatct tccggcgtgg cgttgcggct acccatcatc
     6721 gtcgtttctt ttttatggaa ttccggatcg gagaactgca actcaccttt aaacaggccg
     6781 acaaatacca ccgtgccgcc gtgacgaatc aaattcacgg tgttattcat cgcatgttga
     6841 ttacctgtcg cgtcgatcac tttctgcgcc agcgaaccac caaactgcgc ccgtagctgc
     6901 gcgtcaaaat cttcggctga cgggtccagt aaaggtaatt ccagacgcgt tgccacatgt
     6961 tcacggcgcg ccggactggt atccgccacc accacctgtg cgccatc
//
LOCUS       DAJTZL010000189         6905 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.190, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000189 DAJTZL010000000
VERSION     DAJTZL010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6905
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.190"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(308..889)
                     /locus_tag="OO544_003570"
     CDS             complement(308..889)
                     /locus_tag="OO544_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6769085.1"
                     /translation="MYYPVTDYIALALIISFLFLTLFICLLCLKHERIKKETIRQKNA
                     HILEHGWNATEFSWFRYGQYNETGIYISIEKTIIITITVSGECFKKEYSIVSHMLVTD
                     TITEATLYENGLYTRHIRLSRPVSDSKYPLPPGSQLIKNMTLRLRLQDQQEETSVTLF
                     QGKMSTDGNNYYIIKGKVSSVLLLLKMLQINHA"
     gene            complement(936..4793)
                     /gene="sigA"
                     /locus_tag="OO544_003571"
     CDS             complement(936..4793)
                     /gene="sigA"
                     /locus_tag="OO544_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SigA"
                     /protein_id="HCR6769086.1"
                     /translation="MNKIYSLKYSHITGGLVAVSELTRKVSVGTSRKKVILGIILSSI
                     YGSYGETAFAAMLDINNIWTRDYLDLAQNRGEFRPGATNVQLMMKDGKIFHFPELPVP
                     DFSAVSNKGATTSIGGAYSVTATHNGTQHHAITTQSWDQTAYKASNRVSSGDFSVHRL
                     NKFVVETTGVTESADFSLSPEDAMKRYGVNYNGKEQIIGFRAGAGTTSTILNGKQYLF
                     GQNYNPDLLSASLFNLDWKNKSYIYTNRTPFKNSPIFGDSGSGSYLYDKEQQKWVFHG
                     VTSTVGFLSSTNIAWTNYSLFNNILVNNLKKNFTNTMQLDGKKQELSSIIKDKDLSVS
                     GGGELTLKQDTDLGIGGLIFDKNQTYKVYGKDKSYKGAGIDIDNNTTVEWNVKGVAGD
                     NLHKIGSGTLDVKIAQGNNLKIGNGTVILSAEKAFNKIYMAGGKGTVKINAKDALSES
                     GNGEIYFTRNGGTLDLNGYDQSFQKIAATDAGTTVTNSNVKQSTLSLTNTDAYMYHGN
                     VSGNISINHIINTTQQHNNNANLIFDGSVDIKNDISVRNAQLTLQGHATEHAIFKEGN
                     NNCPIPFLCQKDYSAAIKDQESTVNKRYNTEYKSNNQIASFSQPDWESRKFNFRKLNL
                     ENATLSIGRDANVKGHIEAKNSQIVLGNKTAYIDMFSGRNITGEGFGFRQQLRSGDSA
                     GESSFNGSLSAQNSKITVGDKSTVTMTGALSLINTDLIINKGATVTAQGKMYVDKAIE
                     LAGTLTLTGTPTENNKYSPAIYMSDGYNMTEDGATLKAQNYAWVNGNIKSDKKASILF
                     GVDQYKEDNLDKTTHTPLATGLLGGFDTSYTGGIDAPAASASMYNTLWRVNGQSALQS
                     LKTRDSLLLFSNIENSGFHTVTVNTLDATNTAVIMRADLSQSVNQSDKLIVKNQLTGS
                     NNSLSVDIQKVGNNNSGLNVDLITAPKGSNKEIFKASTQAIGFSNISPVISTKEDQEH
                     TTWTLTGYKVAENTASSGAAKSYMSGNYKAFLTEVNNLNKRMGDLRDTNGEAGAWARI
                     MSGAGSASSGYSDNYTHVQIGVDKKHELDGLDLFTGLTMTYTDSHASSNAFSGKTKSV
                     GAGLYASAIFDSGAYIDLISKYVHHDNEYSATFAGLGTKDYSSHSLYVGAEAGYRYHV
                     TEDSWIEPQAELVYGAVSGKRFDWQDRGMSVTMKDKDFNPLIGRTGVDVGKSFSGKDW
                     KVTARAGLGYQFDLFANGETVLRDASGEKRIKGEKDGRILMNVGLNAEIRDNLRFGLE
                     FEKSAFGKYNVDNAINANFRYSF"
     gene            complement(5154..6158)
                     /locus_tag="OO544_003572"
                     /pseudo
     CDS             complement(5154..6158)
                     /locus_tag="OO544_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003557916.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
     gene            6143..6520
                     /locus_tag="OO544_003573"
                     /pseudo
     CDS             6143..6520
                     /locus_tag="OO544_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014839879.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6559..>6905)
                     /locus_tag="OO544_003574"
     CDS             complement(6559..>6905)
                     /locus_tag="OO544_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000555401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6769087.1"
                     /translation="VPGEYSSGNSIRPRGITKVGNSELRRLLYEAAWSYRTPAKVGAW
                     LIYYRPDSVTQYSKDIAWKAQQRLCSRYRTLTAKGKKSQVAITAVARELTGFMWDIAL
                     AAQSSFSQQKQN"
BASE COUNT         1723 a         1531 c         1558 g         2093 t
ORIGIN      
        1 taccaatact ggcttaatgg ctacatacgg cagtcggttt acgcttacct ccgttaacgc
       61 ttacctccgt taacgcttac ctttaatctg ctgaatttcg aattgttcag gagtaatagt
      121 ccaaagtatc gggaattttc cagacaatcc tgataatacg cttatgaaca ttacaacacg
      181 ctcatgcgga acctggatgg gggaaggaac ggtgaagctg ttagcgaatg accggcgttt
      241 gtataaacgc cggtcatgat gaatgatatt tcagaatata cctcgggcta aaataccggg
      301 acagagatca ggcgtgatta atctgcagca tttttaataa cagaagtact gagcttactt
      361 tacctttgat gatgtaatag ttatttccgt cggtactcat ttttccctga aataatgtca
      421 cggatgtttc ttcctgctga tcctgcaggc gcagtcgtaa ggtcatattt ttaatgagct
      481 gagatccggg tggcaagggg tatttgctgt cagatactgg gcgactgagt ctgatgtggc
      541 gcgtatacag cccgttttcg tacagggtgg cctctgttat ggtgtcagtg accagcatgt
      601 gggagacaat gctgtattct tttttaaagc actcaccaga aacggtgatt gtaatgataa
      661 tggtcttctc gatcgagatg taaatacccg tttcgttata ctgtccgtat cggaaccatg
      721 agaactcagt tgcattccag ccatgctcca gaatatgtgc atttttttgc ctgatagttt
      781 ctttttttat tcgctcatgt ttaaggcata acaggcagat aaataatgtc agaaaaagaa
      841 agctaataat gagagcaaga gcgatatagt ctgtcacagg gtaatacata ttctctacgg
      901 tagaagaagg gccgcaaacg cggcccgggc tgttatcaga aagagtaacg gaagttggcg
      961 ttgatcgcgt tatccacgtt gtatttacca aatgccgatt tctcaaactc aagaccgaag
     1021 cgaagattat cgcgaatttc ggcgttgaga ccaacattca tgagaatacg accgtctttt
     1081 tcacctttga tacgtttctc accggacgca tcacgcagta cggtttcacc gttggcaaac
     1141 aggtcaaact ggtagccaag gccggcgcgg gctgtgactt tccagtcctt accggagaag
     1201 gatttaccca catcaacacc ggtacgccca atcagcggat taaagtcctt atccttcatg
     1261 gtcacgctca ttccgcgatc ctgccagtcg aaccgtttac ctgatacggc cccataaacc
     1321 agttctgcct gcggctcaat ccaggagtct tctgttacat gatagcggta gcctgcttca
     1381 gcacccacat acaaggaatg agaactgtag tcttttgttc cgagtccagc aaaggtcgcc
     1441 gagtactcat tatcatggtg aacatactta ctaatcaggt cgatataggc accagagtca
     1501 aatatagcgg aagcatacag acctgccccg acggacttcg tcttgccact gaatgcatta
     1561 ctgctggcat gactgtcggt atacgtcata gtcagaccag tgaaaaggtc aagtccatcc
     1621 agctcatgtt ttttatccac accaatctgc acatgggtgt agttgtcact gtatccacta
     1681 gaagctgaac ctgctccgct catgatgcgg gcccatgcac cggcctcgcc attggtgtca
     1741 cgcagatccc ccattcgttt attcaggttg ttgacttctg tcaggaaggc tttgtaatta
     1801 ccggacatat acgattttgc tgcaccggaa gatgctgtat tttcagccac cttatatccg
     1861 gtcagggtcc acgtggtatg ttcctgatcc tctttcgtgc tgatcacagg agatatgttg
     1921 ctgaaaccta tggcctgagt actggcttta aatatctctt tattgcttcc ttttggggct
     1981 gttatcaggt caacgtttaa tcctgagtta ttatttccca ctttctgtat atcgaccgac
     2041 agactgttat tgcttccggt taactgattt ttaacaatga gtttatccga ttgatttaca
     2101 gactggctca gatcagcccg cataatcaca gcagtattag tggcatccag tgtgtttact
     2161 gtcacagtat ggaaacccga attctctatg ttactaaaca acaaaagact gtcgcgggtt
     2221 tttaatgatt gcagggctga ctgtccgttt actctccata aggtgttata catgctggct
     2281 gaggcagcag gagcatcaat acctccggta taagaagtat caaagccacc cagcaaacct
     2341 gtagccagcg gtgtgtgtgt ggttttgtcc aggttatctt ctttatactg gtcaacacca
     2401 aacagaatag atgctttttt gtctgatttt atattaccat tgacccaggc ataattttga
     2461 gcctttaacg tggcaccatc ttctgtcata ttatatccat ctgacatata gattgccggg
     2521 ctgtatttat tattttctgt aggggtgcct gttaatgtca gggttccggc cagttcaata
     2581 gctttatcta catacatttt tccctgggcg gtaacagtag ctcctttgtt gataatcagg
     2641 tctgtattaa ttaaggataa tgcaccagtc atagtaacag ttgatttatc accaacagtt
     2701 attttgctgt tttgagcact cagactgccg ttgaaactac tttcgcctgc tgaatccccg
     2761 gagcgaagct gttgtctgaa tccaaaacct tcgccagtaa tgtttcttcc tgagaacatg
     2821 tcaatgtatg cagttttatt tcccagaaca atttgagagt ttttagcctc tatgtgtcct
     2881 tttacattag catcccggcc tatactcagg gttgcgtttt ctaaatttaa tttccggaaa
     2941 ttaaatttac gactttccca gtcgggctgg gaaaaagagg ctatctgatt gttggactta
     3001 tattccgtat tgtaacgttt atttacagtg ctttcctggt cctttatggc agcagaatag
     3061 tctttttgac ataaaaaagg aattggacag ttgttattgc cttctttaaa tatggcatgt
     3121 tctgtcgcat gtccttgtaa tgttaactgt gcattccgga cagagatatc gtttttgata
     3181 tcgactgagc catcaaagat cagattggca ttattgttat gttgctgggt agtattgata
     3241 atatgattta tgcttatatt acctgataca ttcccatggt acatatatgc atcagtatta
     3301 gtaagtgata atgttgattg cttcacgttt gagttcgtta cggttgttcc cgcatctgtt
     3361 gctgcgattt tctgaaatga ctggtcatag ccgtttagat ccagtgttcc gccatttctg
     3421 gtaaaataga tttcgccatt accgctttcg cttaaagcgt ctttggcatt tatttttacc
     3481 gtacctttac ctccggccat gtaaatttta ttgaaggctt tttcagcact aaggatgaca
     3541 gtcccattac ctattttaag gttatttccc tgtgctattt ttacatccag agtaccacta
     3601 cctattttat gcagattatc tccggcaacg cccttaacat tccattcaac ggtggtatta
     3661 ttatcaatat ctatcccggc acctttataa gacttatctt ttccgtacac tttatatgtc
     3721 tggttcttat cgaatataag cccgccaatg ccaagatcgg tatcctgctt gagcgttaat
     3781 tcccctcctc ctgagacaga caggtcctta tcttttataa tcgatgataa ctcttgtttt
     3841 ttaccatcca gctgcatagt gtttgtgaaa ttctttttta aattgtttac cagaatatta
     3901 ttaaataacg agtagtttgt ccaggctata ttggtactac tgagaaaacc aactgtactg
     3961 gtaacaccat ggaaaaccca tttttgttgt tctttatcat atagataaga accagaacca
     4021 ctatcgccaa aaattggtga gtttttaaaa ggggttctgt tggtataaat gtaactcttg
     4081 tttttccagt ccagattaaa aagacttgcg cttaacaagt cgggattata gttttgtcca
     4141 aacagatatt gtttgccgtt taatatcgtt gaggttgttc cggcacctgc tctgaagcca
     4201 attatttgtt ccttaccgtt gtagtttacg ccatatcttt tcatcgcatc ttcgggagaa
     4261 agtgagaagt cggcactctc cgtaacccct gttgtttcca cgacgaattt attcagacga
     4321 tgaaccgaaa agtcgccaga tgatactctg ttacttgctt tatatgctgt ctgatcccat
     4381 gactgtgttg ttattgcatg atgctgtgta ccgttatgag tcgcagtaac actgtacgca
     4441 cctccaattg atgttgttgc acctttgttg gaaacagcag aaaaatcagg tacaggtagt
     4501 tctggaaaat gaaatatctt tccatctttc atcattaatt gaacatttgt tgcacccggt
     4561 ctgaactctc ctctgttttg agcaaggtca agataatcgc gggtccatat attatttata
     4621 tccagcattg ctgcaaatgc tgtttcgcca taacttccat atattgagga taaaataata
     4681 ccgaggataa ctttctttct tgatgtaccg acactaactt tccgggtcag ttcagaaaca
     4741 gcaactaatc cacctgtaat atgactatat ttcagtgaat aaattttatt catacgtgaa
     4801 ctccgatatt tctgagttgt ggttatggga tagcatttaa ttacaacggg gaactatcag
     4861 tcatggtacg taactgataa gggtataatg ataatcactg tcagttatat ttgtatgaca
     4921 gatgtaacaa aatgaaaaaa aagataagca caagtgattg attggtcaga tattgatcac
     4981 ttggtgtttg tattttgaac tttgcgatca aaatttgatc tttgttgttt ccggtcaatc
     5041 ctttacaggt tttctgtatt atccggggga gttaagtctg tcgatgtccg acagactttt
     5101 gatgatggaa acaacatgca gcgataatgt gcatgctgtt ttgcaggtgg aactcagaag
     5161 gtgaactgga actgtgcgac cgcaccgtaa gtgttgtccg tgccgatcag gcctgcaaca
     5221 tcaaggtgaa ccacatcaaa cggtgagatc cccaccccgg ccgtgaatgc actgccgtcg
     5281 ttaccggcca gattctgacg gtaaccggcg cgcagctgtg cccatttcca ggcattaaat
     5341 tctgcgccaa tggcggcaaa ctgacggttg ctgtcggagg tgaaaccgct ggccggggtc
     5401 agatccacat caaatgcggt ggtgaacatc gcgttgtgcc aggagacacc ggccgtcacc
     5461 tgcgggcgga tcctgaaggt ttccgtgata ccgttcactt cttttgtctc aatactacgg
     5521 gggatcaggt tctgtgcgac cagtcccagc gtccagttgt catccaggtc cgtactggca
     5581 ccgatgtcgg cgttgatgcc atttttcgtg ttgtggtagc ggtcgccttt gaaggcgctg
     5641 ctgtcataat ttctgaccag cacgttgtag ttgaacaggt cgacgcgctg gaatttgggg
     5701 gggaagccca gagaccattt ctgcccggcc gtttccagtt ccgtagccag agcaataccc
     5761 acatcagtga tcagtgccgc gcgggcaaag gcctgtgatg tcaggcggct tttgtccacc
     5821 tcctgcccgg tgctgtttgc cacctcttcc aggtaagtga ggtcagcatc agagacttta
     5881 ccgtcgacac tgacggtgcc gtgtgattta accatcagtg cggcagagag gcgatcaccg
     5941 ggaagggccg ccacagcaga aactcccagc tgtgcggcgg catgtgtgtt acggaagtcc
     6001 cggaggtgtt ctttcaggcg tgcggcagcc tcaggaacac cgtggttact ttcgatggcg
     6061 cggtcgaagg ccttccactc atcactgatc cggtcggcgt tgtcggtgat attatccgga
     6121 ttggagagct gcgcgccgac ggacggaagc aggatgctgg gctcatgcgc gacgcaaaat
     6181 ccacgatgta tatatcagta ctaaaagcgc gacggcggaa gaagctctga aactaatcgg
     6241 tgaactgtac gccatagagc acgatatacg cgggttaccg gtgtctgaac gcctggcggt
     6301 caggcaaatg cagagtagac cgctcctgac ttccctgtat aagctgatgc aggagaaaga
     6361 acacacgtta tcgaagaaat gtcgtctggg agatgcgttc cggtatatca ggaagcactg
     6421 ggctgcgttg tgcaacttct gtgatgatgg cctggctgag gtagataata atgcagcaga
     6481 aagagcgctt cgtgcagtct gtctcgggaa gaaaactttt tcgaggatca ctcattccgt
     6541 gtctgactgc ttcagggttt aattttgctt ctgctgactg aatgatgatt gggcagcaag
     6601 tgcaatatcc cacataaatc cagttaactc acgagccacc gccgtaatgg ctacttgtga
     6661 ttttttccct tttgctgtca gagttcggta acgagaacac aatcgttgtt gagctttcca
     6721 tgcaatatct ttggaatatt gtgttacaga gtccggtcgg taatatataa gccatgctcc
     6781 aacttttgca ggtgtacgat aagaccaagc ggcttcgtaa agcagacgtc tcagttcgct
     6841 gtttccaact tttgttattc ctctgggacg aatactgttt ccactggaat attcacctgg
     6901 cacca
//
LOCUS       DAJTZL010000190         6866 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.191, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000190 DAJTZL010000000
VERSION     DAJTZL010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6866
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.191"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..1337)
                     /gene="tar"
                     /locus_tag="OO544_003575"
     CDS             complement(<1..1337)
                     /gene="tar"
                     /locus_tag="OO544_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HCR6769088.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNIDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVARVDTGSVLVESAGETMNNIVNA"
     gene            complement(1482..1985)
                     /gene="cheW"
                     /locus_tag="OO544_003576"
     CDS             complement(1482..1985)
                     /gene="cheW"
                     /locus_tag="OO544_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HCR6769089.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(2006..3970)
                     /gene="cheA"
                     /locus_tag="OO544_003577"
     CDS             complement(2006..3970)
                     /gene="cheA"
                     /locus_tag="OO544_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HCR6769090.1"
                     /translation="MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASFDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQSPR
                     RIILSRLKAGEVDLLEEELGHLTTLTDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKISTPPVLKLAAEQAPTGRVEREKTTRSNESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSMAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVGDEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(3975..4901)
                     /gene="motB"
                     /locus_tag="OO544_003578"
     CDS             complement(3975..4901)
                     /gene="motB"
                     /locus_tag="OO544_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HCR6769091.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEDLKKRMEQSRLRKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRVIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(4898..5785)
                     /gene="motA"
                     /locus_tag="OO544_003579"
     CDS             complement(4898..5785)
                     /gene="motA"
                     /locus_tag="OO544_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HCR6769092.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGGVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(5911..6489)
                     /gene="flhC"
                     /locus_tag="OO544_003580"
     CDS             complement(5911..6489)
                     /gene="flhC"
                     /locus_tag="OO544_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HCR6769093.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(6492..6842)
                     /gene="flhD"
                     /locus_tag="OO544_003581"
     CDS             complement(6492..6842)
                     /gene="flhD"
                     /locus_tag="OO544_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HCR6769094.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
BASE COUNT         1588 a         1856 c         1687 g         1735 t
ORIGIN      
        1 gcattgacga tattgttcat tgtttccccg gcgctttcga ccagcaccga accggtatca
       61 acgcgtgcga cggagtcttc aatgagggct ttgatctctt ttgccgcctg ggcgctgcga
      121 ctggcaagat tacgcacttc acccgccacc acggcaaaac cacggccctg ttcacccgca
      181 cgcgcggctt caaccgcggc attcagcgcg aggatattag tctggaaggc aataccgtcg
      241 ataacgctga taatgtcggc aattttcttc gaactatcgg cgatctcatg catcgttttc
      301 actacgccat ccaccacttt gccgccgtgc tgggcggtgt cggaggcact ttgcgccagt
      361 tgcgaggcct ggcgggcgtt atcggcgttt tgcttcactg tcgcggtgag ctgctccatg
      421 ctggcggcag tttcttccag cgcggatgcc tgctgttcag tacgggagga aagatcggtg
      481 ttgcccgccg caatttcacg ggtaccggca tagatggcat ctgaaccttc gcggacatga
      541 gtgacggtgt cagtcaaaga gcgttgcata tgtgaaacgc tctgcgccag gtcgcccatt
      601 tcactgcgcc cgtcaatggt cagggtattc gctaggttac caccggcgat ttcgcgaatg
      661 tgagcaataa tttttgccag cggagtaagc aacatacggc gaatgccgta ccacgccacc
      721 agcagaatca ataccaccac cagcgcgata accgccagtt gccactgggc aaatcggtaa
      781 tcatctgcgt tgtcagtgac gatatcgcga tacagttttt cactgctgag ggcgtactga
      841 gcaaacgctt cgcccattgc attttgcatt ccctgggttg gctgagcgaa ataagctcca
      901 gtattgccat aatctagata atcaatcagt tcagttaacg ctgtgtaata gtttttatat
      961 ttttcatcaa tattacgact ggtagcgacc atttcaggta acggtgccat gcttttgaat
     1021 tttttataat gcgtcgctgc ctgcgccaat gttttcctgg cgctatcgag caattcaact
     1081 ttggcgttac tttgttgatt ggaggaatcc atcatcatcc gcaccgctga acgactcagg
     1141 ttaatgcgcg tttgcagcat taaatcccag gttgacgtca gctcgccctg ctgttcccgc
     1201 aattgattgg aaaccacaaa gctcttctgg ctatggtgaa gggaagaaaa aaacagactg
     1261 ccggaaataa gctgtaacag tgcgaatacc cccagcacca ttaccaacag cgtgactacg
     1321 cggatacggt taatcataag gcaccttcct gaaaacaagt tgatctcgtt atcggcaaga
     1381 aggggggaaa ctttattgct gatgccaccc gccgcgaaat tgaaataaaa aacccgatgc
     1441 gcagatcatc gggttcattt caattgagga aatcgggaga attacgccac ttctgacgcc
     1501 gcgctatcta acagcgccat ctcttcgctg ttcagcagtt tttcgatgtt caccagaatc
     1561 aacatacggt cgcccagtgc gcccagtcca gtgagatatt ctgttgaaag cgtcacggca
     1621 aactctggtg ccggacgaat ttgctccgcc gtcaatgaaa gcacgtctga aacgccgtca
     1681 accacgatgc cgaccacccg ctgtccgaga ttcaggacga taactaccgt gttgtcgtta
     1741 tagtccacat ccacctggct gaacttaatt cgtaagtcaa caatcggcac aataacgccg
     1801 cgcagattcg tgacgccttt gataaacgct ggcgtgttcg caatccgtgt tacctgatcg
     1861 tagccacgga tctcctgcac tttcaggata tcaataccgt actcttcatc accaagggta
     1921 aataccagaa attcctggcc tgacggctcg ctggccagct ttgttacatt cgtcataccg
     1981 gtcatattgt taccttttta ctcattcagg cggcggtgtt cgccatacgt tgttcgcggt
     2041 ttatcgcctg caaggcggag acatcaacaa tcagtgccac gctgccgtcg ccaagaatgg
     2101 tcgcagcaga aatgccgggg actttgcgat agttactttc aaggttttta accacaacct
     2161 ggtgttgacc aattaattga tccaccagca aggcgtagcg gcgaccgcca ctttgtaaga
     2221 tcacaacaat tccctgggtg gcttcggttt tcgcgcccgc gacgttgaac actttccaca
     2281 gttcgacgat gggcagatat tcaccccgca cttccagtac ccgctcgccg ccggccagtg
     2341 gatggagatc ggcttcacgg ggttgcagtg attccataac agcattcagc ggcagaatga
     2401 aaacttcatc cccaacgcgt acggacatgc cgtcgaggat ggccagcgtc agcggcagta
     2461 aaatgcggat cgtagtgcca gtcccctgct tcgactggat ttcgacatga ccgcccatct
     2521 cctggatatt acgtttaacg acgtccatgc cgacgccgcg cccggagacg tcggtgacct
     2581 gctctgccat ggagaagcct ggtgcaaata tcagcatcgc gacttcgtcg tcgctcatgt
     2641 tttcgctgac agtcaaacct tgcgaggccg cttttgccag aattcgctca cggtttagcc
     2701 ccgccccatc gtcggtcact tcaatgcaaa tgttgccgcc ctgatgttcg gcagacagaa
     2761 ttaaatttcc gacgctgttt ttacctgcgg cgagccgttt ttctggcagt tcaataccgt
     2821 gatcgaggct attgcgtacc aggtgggtca gcgggtcgat aatgcgttct atcaggcttt
     2881 tgtcgagttc agtagaactg cccaccagcg tcagttctac ctgcttgccg agttttcccg
     2941 ccagatcacg caccagccgg ggatagcgac taaaaacata ttccatcggc atcatgcgaa
     3001 tcgacatcac tgattcctgc aaatcacggg cgttacgttg taactgcccc atgctggtta
     3061 tcaaatcacc atgattaacc gggtccagtt cgctggaacg ctgggcaagc atggactggg
     3121 tgataaccag ctcgccgacg aggttaatta attgatcaac cttttctacc gctacacgga
     3181 tgctggtgga ttcattgctg cgcgtcgttt tttcccgctc cacgcggccg gttggcgctt
     3241 gttcggctgc cagtttaagc actggtgggg tggatatttt tggcgagact tctactgttt
     3301 caaaggtaat ctgatcggct tcaatcacaa aacagagtac cgctgtgatg tcatcttcgg
     3361 cgatgtcgcc cggtaatatt gccgagagcg aatccgcccc tttcaccacg tcagttaacg
     3421 ttgtcagatg tcccagttct tcttccagca ggtcgacttc cccggccttc aggcgcgaaa
     3481 ggataattcg tcgcggcgac tgactgcgac tctgctcatc ttgcggttca cttttggcaa
     3541 ccacacttaa tcgggtcact gcggatggcg tttcgccttt cgcttctaat gccagttgac
     3601 gcaaggcctg gcagatataa tcgaagctgg cggcatccgg ctcttgcgac tgtttataag
     3661 cgtcgagctg ttcttgcatg atgtccttcg tttccaaaaa cagattgata atgtcggtgt
     3721 tgagttgcat ctcacctcgt ctggcttcat cgagcaggtt ttccatcaga tgcgtggttt
     3781 cctgcaaaac gctgaagcca aaagttcctg cccctccttt gatcgagtgg gcagcccgaa
     3841 agatggcatt caattgttcg gcatctggcg cttccggctg caaaaccagc agatgctgct
     3901 ccatgtcagc caacagttcg tccgcttcat caaaaaatgt ctgataaaaa tcgcttatat
     3961 ccatgctcac gctgtcacct cggttcggct gatggcattg tgggaacact gacctgtggt
     4021 gcaacctcag gtttttccag ggcgcttact ggctcattct ggctttcggc gttttcatgc
     4081 aaaatggcct gttcggcttg tttgttcagt accagcaggc tgatgcgacg gttgacggca
     4141 tcatcaggtc cgcgatcgct taagcgcatc gttgccgcca tgccgacgac acgtaacact
     4201 ttgccgctat ccaaccctcc gaccatcagt tcgcggcggg atgcattggc ccgatcggca
     4261 gaaagctccc agttgctata tcctttctca ccgctggcgt aggggaaatc atcggtatga
     4321 cctgaaaggc taatacggtt gggaataccg ttcagtacag gcgcaatgac acgcagaatg
     4381 tcgcgcatat agggttcgac atcggcactg ccggttctaa acatcgggcg attctggcta
     4441 tcgatgatct gaatacgtag accttcctgg accagatcga ttttgagatg gggacgtaac
     4501 gcccgcagtt tcggatcgga ctctatcaac tggtcgagat caccccgcaa tttccgcaat
     4561 cgactttgct ccatgcgttt tttcagatct tcgatgttcg gctgcttatt cacttccccc
     4621 tggctttggg tgtaatcatc accaccgccg ggaattgggc tttcactatt agaaatgcga
     4681 tcgccgcccg taaccgcagt cgccagtgga gtccggaagt actccgcaat ctgaatcagc
     4741 tcttttgggc tggagatgga gatcagccac atcaccagaa aaaaggccat catcgcagtc
     4801 ataaagtcgg cataagcaat cttccacgat ccatgtgctg ccccgtggct tttggctttg
     4861 cgtcgtttga cgacaataat cggatgcgct tgattcttca tgcttcctcg gttgtcgtct
     4921 gttgttgcgg atttttcacc gcacgcacat gctcttccag ttcaataaac gacggacgtt
     4981 cgctggaata gagcgttttg cgaccaaact caacggcgat aggcggtgcg taaccgttca
     5041 gattagaaag cagagtgact ttgacgcact gcatcatttt gctggtttcg gcgcttttct
     5101 gacgtaaaac actcgctaat ggggaaataa atccgtaagc caataaaatg ccgaggaaag
     5161 tccccaccat cgcatgtgcg ataagcgcac ccagctcggc ggcaggacga tcggctgaac
     5221 ctaacgcgtg aacgcccccc attacagccg cgacaatacc aaacgccgga agtgagtccc
     5281 cgaccagcgc cagactgttc gccgggactt ctgcctcgct ttcgtgcgtc tcaatctctt
     5341 catccatcag ggcttcgatt tcgaaggtgt tcatatgacc gctgataatc agacgcagat
     5401 aatcgacgat aaaatcaagc atgacgctat ccgcgaggat gcgtgggtag ctggcgaaga
     5461 tctcgctctc acggggattt tcaatatcac gttccagcga aaacatcccc atctgccgcg
     5521 atttcgccat caaccgataa agcagagcca gcagatccat atacattgct ttggtgtatt
     5581 tggagcgacg aaacagcaac ggcagcgcct tcagcgtgcc tttaatcgct ttgccattat
     5641 tgccgacgat aaacgaccca atccctgcac cggcaataat caccagttca gcgggttgat
     5701 agagtgctcc aaggcttcca ccggtcatca aataaccgcc gaaaactgta ccgagaacaa
     5761 ccaggtaacc taataagata agcacgacat catccttcca ctgttgacca tgacaggatg
     5821 ttcagtcgtc aggcgttaac gcgcgattgg ggcaaaaaaa gcagcggtac gtcgttaccg
     5881 ctactggaat gttgcgcctc accgtatcag ttaaacagcc tgtactctct gttcatccag
     5941 cagttgtggg ataatatcgg caggattctg ggaaagttta cgtcttttta ctgcccggga
     6001 tggcggttga cataagctgc aggcaaagct gccaacaggc tggtgagcgt gggtaataaa
     6061 attgccgccg cagcagttgc agctggaaag ttgcagtaat ccactttcaa caaaccgcac
     6121 caatgtccag gcacgggtta atgccagcag tggtccttct tctgcttgtg ggcactgttc
     6181 aaggtataaa cggtaggctt tgatcaccgc atcgacgcca ttacacaaac cggttttcag
     6241 taaaaactgc catgcattac agaacatcga agcatgaacg ttttgttccc aggtcataaa
     6301 ccagtcggtt gagaatggca gcatgccttt cggcggtggg cttccgcgca gttctttata
     6361 aagttttatc aggcgtccgc gacttaactg tgtttcgctt tccagcatct gcaaacgagc
     6421 gcccagggta atcaattcca ttgccagctg aatatcccgc gcttcctgaa caatgctttt
     6481 ttcactcatg atcaggccct tttcttgcgc agcgcttctt caggctgatt aacatcattc
     6541 agcaagcgtg ttgagagcat gatgccggta tgaatttgct ggagatcgtc aacgcgggaa
     6601 tcttgcgtca actgagtaat cgtctggtgg ctgtcaaaac ggaagtgaca aaccagttga
     6661 ttggtttctg ccagcttaac catttgcgga agagtcagtg ccgctaacgt tgtcgccatt
     6721 tcttcattta tgccgagacg aaacatagcg gacgctttgt cctgaacaat caaacgctgt
     6781 gcaagtagta aatatgacaa gttgatgtca taaatgtgtt tcagcaactc ggaggtatgc
     6841 attattccca cccagaataa ccaact
//
LOCUS       DAJTZL010000191         6863 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.192, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000191 DAJTZL010000000
VERSION     DAJTZL010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6863
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.192"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..353)
                     /gene="rnpB"
                     /locus_tag="OO544_003582"
     ncRNA           complement(<1..353)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="OO544_003582"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(386..1531)
                     /gene="garK"
                     /locus_tag="OO544_003583"
     CDS             complement(386..1531)
                     /gene="garK"
                     /locus_tag="OO544_003583"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HCR6769095.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSVPVADG
                     GEGTVEAMIAATQGAERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESSATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG"
     gene            complement(1628..2518)
                     /gene="garR"
                     /locus_tag="OO544_003584"
     CDS             complement(1628..2518)
                     /gene="garR"
                     /locus_tag="OO544_003584"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HCR6769096.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLLLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(2548..3318)
                     /gene="garL"
                     /locus_tag="OO544_003585"
     CDS             complement(2548..3318)
                     /gene="garL"
                     /locus_tag="OO544_003585"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HCR6769097.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRTNMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            complement(3334..4668)
                     /gene="garP"
                     /locus_tag="OO544_003586"
     CDS             complement(3334..4668)
                     /gene="garP"
                     /locus_tag="OO544_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HCR6769098.1"
                     /translation="MILDTVDVKKKGVHTSYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGGIGFVLTALWIKLIHNPTDHPRMSAEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     VSTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            5043..6614
                     /gene="garD"
                     /locus_tag="OO544_003587"
     CDS             5043..6614
                     /gene="garD"
                     /locus_tag="OO544_003587"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HCR6769099.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHTPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPAPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLVGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGVQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEESIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            6763..>6863
                     /gene="prlF"
                     /locus_tag="OO544_003588"
     CDS             6763..>6863
                     /gene="prlF"
                     /locus_tag="OO544_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PrlF"
                     /protein_id="HCR6769100.1"
                     /translation="MPANARSHAVLTTESKVTIRGQTTIPAPVREAL"
BASE COUNT         1682 a         1883 c         1689 g         1609 t
ORIGIN      
        1 gttctgtcgt ggacagtcat tcatctaggc cagcaatcgc tcactggctc aagcagccta
       61 cccgggttca gtacgggccg taccttatga acccctattt ggccttgctc cgggtggagt
      121 ttaccgtgcc acggactgtt accagccgcg cggtgcgctc ttaccgcacc ctttcaccct
      181 tacctgatcc cgcttgcgcg ggccatcggc ggtttgctct ctgttgcact ggtcgtgggt
      241 ttccccccca ggcgttacct ggcaccctgc cctatggagc ccggactttc ctcccctccg
      301 cccgtctccc ccgaagagga cgacgacgaa gcggcgactg tctggtcagc ttcggcgcgc
      361 agtatagagg gtttgcgcgc ccttgtcacc ccgcgttgcg cattccaatc gccagtgtcg
      421 cggcgatatt acgtgaagca cggcagatat tgtcataagc cccgcggaat gcttcgtcca
      481 acgtacctat gctggtcaat acgctgaaga ccgcatcgat gccatgctga tgtacaacgc
      541 caacatcatc ggtcaggcta cccgcaatgc caatcaccgg tttatggtac ttcttcgcca
      601 cgtttgcaac gccaatcggt accttgccgt gaatactctg gctgtcaata cgcccttcac
      661 cggtgatcac cagcgtacaa tcgtgaatat gttcttccag attcagcgcc gtagtgacga
      721 tttcaatacc acttttcagt tccgcaccaa gaaacgccat cagcgccgcg cccataccgc
      781 ccgcagctcc tgcgccgggg acatctttca catcaacatg cagcgctttt ttaatgacat
      841 cggcatagtg agagaggtta ttgtccagct caacaatcat cgcttcactg gctccctttt
      901 gtgggccaaa gatgcgcgat gcgccgttat cgcccaccag cggattggtg acatcacaag
      961 cgacgcgaat gacgcaatct tttaagcgcg gatcgaggcc ggaaatatca atatcattca
     1021 gagtattaag actaccgccg ccaaaaccaa tttcattgcc gttggcgtcg cataatttcg
     1081 cccccagcgc ctgtaccatg cctgcgccgc catcatttgt agcgctgccg ccaatgccga
     1141 taataatgtt tgttgcactg ctctccagcg cctgcaggat taactcgcct gtgccgcgtg
     1201 aagtggttac gagtggatcg cgtttttccg caggtaccag ctccagccca ctggccgccg
     1261 ccatttcaat aaacgcggtt ttgccatcgc cggagatccc ccaactggca ttcactttct
     1321 cgcccagcgg ccctgtaacc caggcgtgac gttcagcccc ctgggtggct gcaatcatcg
     1381 cttccaccgt tccttcacca ccgtcggcaa ccggaacaga aacatactgt gcatcaggaa
     1441 aaatttcccg aaatcctttt tctatcgcct gcgcaacctc gctggcagat aaactttctt
     1501 tataagagtc tggggcgatt acgattttca tacctatgcc tgttaccaca tgacgccgga
     1561 gggcgtttct cttattcggc ctagattcca ggcccggatt gcaatacacc atccgggcac
     1621 gacgtcatta acgagtaact tcgactttcg ccagtttttc gtagtagcac gccagggcgc
     1681 tatgatccgc cgttcctaaa ccatctgcac gcagtgcctg catcatctcc ataaccgcag
     1741 ctgtgagcag cagttgtgcg ccgacgccgt gagaagtatc cagcgcattc gccagatcct
     1801 taatatgcag atcaatgcgg aagcccggct tgaagttgcg gtccatcacc atcggcgctt
     1861 tggcatccag cacggtactg cccgccagcc caccgcgaat tgcctgataa accaggtccg
     1921 ggttaacgcc cgctttggtt gccagcgtta acgcttctga catcgcagca atattcagcg
     1981 ccacaatgac ctgatttgcc agtttggtga cgttacctgc accgatttcc ccggtatgca
     2041 ccacggaacc cgccatcgct ttcatcaaat catagtattt gtcgaaaata gccttgtcgc
     2101 cgcccaccat cactgacagc gtaccgtcga tggctttcgg ttcaccgccg ctcaccggag
     2161 catccagcat atcaatgcct ttcgctttca gcgcttcgct gatttcacgg cttgccagcg
     2221 gtgcgataga actcatatcg atcaataccg tacctggctt cgcgccttca ataatgccat
     2281 tctcacccag cgccacctct ttcacatgag gggagtttgg cagcatggtt atgatgacgt
     2341 cgcactgttc agcgatcgct ttagccgtag acgctgtttc tgcacctgca gcaatcacgt
     2401 cagcaatagc ttctgggtta cggtcagcaa ccaccagcga gtaacctgct ttcagaaggt
     2461 ttttactcat tggtttaccc ataatcccca ggccaataaa accaactttc atagtcatat
     2521 caaccatctc tcttgttgcg gtggtggtta ttttttaaag gtatcagcca gtttctgagt
     2581 ggcagagcgg aagacgccaa gatcgctgcc gacagccaca aacgtcgcgc cccattccag
     2641 ataacgacgc gcatcggctt cgaccggcgc gagaataccg ctgggtttgc cgtgcgcgct
     2701 ggcacggtta aaaatatgct gaattgcttt ttgtacatcc ggatgcgagg cattgccgag
     2761 atggcccaat gccgcagcca gatcgctggg gccgacgaag ataccgtcta caccttcggt
     2821 agcggcaata gcatcgacgt tatctacgcc ctgctgactt tctatctgga ccagaatagt
     2881 aatgttcttg ttcgactgag cgaaataatc cgccacggtg ccaaacatat tggtgcggtg
     2941 agaaacggag acgccgcgaa tgccttccgg cgggtaacga gttgatgcca ccgcctgctc
     3001 tgcttcctct tttgtttcta caaaaggaat caggaagtta tagaaaccga tatccagaag
     3061 acgcttaata attaccggct cgttggtcgg cactcgcact actggcgcgc tggcgctgcc
     3121 tttcagggcc attaactgcg gaataaacgt ggaaatatcg tttggcgcat gttcgccatc
     3181 cagcaccagc cagtcaaacc cagccaaacc aagaacttca gtgctaatcg ggttagagag
     3241 tgctgaccag caaccaattt gtacctgttt cgcagccagt gcggctttga atttattcgg
     3301 gaaaacatcg ttattcatcg cttatacctt tgcttatttc tgcaattcca tacgtttaat
     3361 gtcgccaact acgaagaggt agcagaccat cgccatcagc gctgaacatc ccacgaaaac
     3421 cagtgctgca ttgaaggagt gcagttcact taccaggtag ccaatcacca gtggagtgac
     3481 aatggaggca acattgccaa agacgttaaa gacgccgcca cagaggccaa caatctcttt
     3541 cggcgcggtg tcagaaatca ccggccagcc cagagcacca aatcctttgc caaaaaaagc
     3601 cagcgccatc agcatgacca ccagcgtggt gttgttggtg tagttacata agatgatggt
     3661 ggaaaccagc aacattccca gcacaatcgg tagcttacgt gccagggtca gggataaacc
     3721 gcgtttgatc agataatccg agaagacacc tcccagcacg ccgcccgcaa aaccacacag
     3781 tgccggaatc gaggcgacca gacccacttt cagaatcgac atgccttttt cctgcaccag
     3841 ataaatcggg aaccaggtga ggaagaacca ggtgatggtg ttgataaaat attgtccgaa
     3901 aaatacgccc aacatcatgc ggttagagag caattgcttg atgtaatgca gtttgggacc
     3961 gcttgctgcc gcgctgcccg gctttttgtg gtccatatcg accaccgcgc cattttcaga
     4021 gataaacttc agctcttccg cagacatacg tgggtgatct gtcgggttat gaatcaactt
     4081 gatccacagc gccgtcagca caaaaccaat cccccccata acggtgaaga cgtgctccca
     4141 gccccaagcg aaagtcagcc agccaagcag cggcgaaaag agcgccagcg agaaatattg
     4201 cgccgagtta aagatggcgg aggcagtacc acgttctttc gtcgggaacc aggcggcgac
     4261 aatacgggcg ttcgccggga atgatggcgc ttccgagaac cccagcataa agcgcataaa
     4321 gaacatggag atccctgccc aggccagcgg gaacatatca acaaagcctt gcaggaaggt
     4381 gaacagcgac cagaaaaaga ggctgtaggt gtaaactttt ttcgagccaa acttatcaag
     4441 caaccagccg ccggggattt gcatcagcaa gtaggcccag ccaaaagcag agaagatgta
     4501 acccatcgaa accgcactta actgcaactc ttttgccact tcggtaccag caatagacag
     4561 cgttgcacga tcggcgtagt taacggcggt aacaataaaa ataatcagta atattaaata
     4621 gctggtatgc acgcctttct tttttacgtc aacggtgtcc agaatcattt tatttacctc
     4681 gggtacttat gctgattttt attattatgg ggaaggtgtt atttatgagt tttatttatg
     4741 ccgttacgac aatgaactcg ggaattagta taagcagcgg gagaataata atcattgtgc
     4801 aaatgctaat ttaatcagta ctatttaaat attattttga gcatatgcac ataaggttgc
     4861 gcgctaaagc acagatttgc gctttacctt accgggcggc actgcaatcc ctgaaatgat
     4921 tgacattgat cacatttctg cgtttaaact cctgacattc ttatttcacc caatgaagtc
     4981 atttattttt aaatgagacc agatcctcat tttaataacc cctggctgga gaatattgca
     5041 caatggccaa catcgaaatc agacaagaaa cgccaactgc gttttatata aaagttcacg
     5101 acacagataa tgtggcaatt attgttaatg ataatggcct gaaagcagga acgcgttttc
     5161 cggatgggct ggaattaatt gaacatactc cccaggggca taaagtcgca ttgctggaca
     5221 ttccagctaa tggtgaaatt attcgttatg gcgaagtgat tggttacgcc gtgcgcgcaa
     5281 tcccacgcgg aagctggatc gacgaatcaa tggttgtact accggaagcg ccgccgttac
     5341 acacgctgcc actggcaacc aaagtcccgg cccccttacc gccgctggaa ggatacacct
     5401 ttgagggcta tcgcaatgcc gatggcagcg tgggcaccaa aaacctgctc ggcatcacca
     5461 ccagcgtcca ctgtgtggca ggcgtggtgg actatgtagt aaaaatcatt gaacgcgatc
     5521 tgctaccgaa atacccgaac gtcgatggcg tggtggggct gaatcatttg tacggctgtg
     5581 gcgtggcgat taacgcaccg gcggcagttg tgcctattcg taccatccac aatatttcgc
     5641 tgaaccctaa ctttggcggc gaagtaatgg tgattggtct gggttgtgaa aagttgcagc
     5701 ctgagcgcct gctggtcggc acggatgatg tgcaagctat tccagtagaa agcgccagca
     5761 ttgtcagttt gcaggatgaa aagcatgtcg gctttcagtc catggtcgag gatattttgc
     5821 aggtcgccga acgccatcta caaaaactga atcaacggca gcgagaaacc tgcccggctt
     5881 cagaactggt cgttggtgtg cagtgcggtg gcagcgatgc gttttctggt gtaacggcaa
     5941 acccggcggt tggctatgcg tctgatctac tggtgcgctg cggcgcaacg gtgatgtttt
     6001 cagaagtaac ggaagtgcgt gacgcgatcc atctgctgac accacgcgca gtgaacgaag
     6061 aggtcggcaa acggctgctg gaggagatgg agtggtacga taactatctc aatatgggaa
     6121 aaaccgaccg cagcgccaac ccttcgccgg gcaacaagaa aggcggtctg gcaaatgtgg
     6181 tggagaaggc actcggctcc attgctaaat cgggtaaaag tgcaattgtt gaagtgctgt
     6241 cgcccggtca acgcccgact aaacgcggat taatttacgc cgcgacgcca gccagcgatt
     6301 ttgtctgtgg cacgcaacag gtggcttcgg gtatcaccgt acaagtgttt acgaccggcc
     6361 gtggtacgcc gtacggcctg atggcggtac ccgtaattaa aatggcaacc cgcaccgagc
     6421 tggcgaaccg ctggtttgat ttaatggata ttaacgcggg cactatcgct accggcgaag
     6481 agagcattga agaggtgggc tggaagttgt tccactttat tctcgacgtc gccagcggga
     6541 agaagaaaac cttctcggat caatgggggc tgcataacca gctggcggtg tttaacccgg
     6601 caccggtgac ctgatttctt ttggaacgtc cctcgcaaaa catggcctta gtgccatgtt
     6661 tttattgttt aaagccccca cgtccattaa taatgcattt gcattacctt agttcaagct
     6721 tataatttga gcagaaaaca ggctgtaaaa ggacaatgaa tcatgcccgc taatgctcgc
     6781 tctcacgctg tactgaccac tgaatcaaag gtcacgatac gcggacaaac aactatcccc
     6841 gcgccagtgc gtgaggcctt aaa
//
LOCUS       DAJTZL010000192         6795 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.193, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000192 DAJTZL010000000
VERSION     DAJTZL010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6795
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.193"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            356..981
                     /gene="gstB"
                     /locus_tag="OO544_003589"
                     /pseudo
     CDS             356..981
                     /gene="gstB"
                     /locus_tag="OO544_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
     gene            complement(978..2093)
                     /gene="yliI"
                     /locus_tag="OO544_003590"
     CDS             complement(978..2093)
                     /gene="yliI"
                     /locus_tag="OO544_003590"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HCR6769101.1"
                     /translation="MHRQSFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRLVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDQGEIPDDNPFIKESGARAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKHYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(2204..2587)
                     /gene="bssR"
                     /locus_tag="OO544_003591"
     CDS             complement(2204..2587)
                     /gene="bssR"
                     /locus_tag="OO544_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HCR6769102.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            2800..4125
                     /gene="rimO"
                     /locus_tag="OO544_003592"
     CDS             2800..4125
                     /gene="rimO"
                     /locus_tag="OO544_003592"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HCR6769103.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPSETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(4172..5493)
                     /locus_tag="OO544_003593"
                     /pseudo
     CDS             complement(4172..5493)
                     /locus_tag="OO544_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            complement(5501..>6795)
                     /locus_tag="OO544_003594"
     CDS             complement(5501..>6795)
                     /locus_tag="OO544_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCR6769104.1"
                     /translation="IAEMKDAEIERSNKLLSLISYDQESGFIKNMAIIESNNNQYLAV
                     GIIKLCGLEAVEAVFGVDERNKIVRKLCQRIAEKYAQCCDIVTFNADLYLLLCRENVQ
                     TFTRKIAMVNDFDSSFGYRNLRIHKSAICEPLQGENAWSYAEKLKLAISSIRDHMFSE
                     FIFCDDAKLNEIEENIWIARNIRHAMEIGELFLVYQPIVDINTRAILGAEALCRWVSA
                     ERGIISPLKFITIAEDIGFINELGYQIIKTAMGEFRHFSQRASLKDDFLLHINVSPWQ
                     LNEPHFHERFTTIMKENGLKANSLCVEITETVIERINEHFYLNIEQLRKQGVRISIDD
                     FGTGLSNLKRFYEINPDSIKVDSQFTGDIFGTAGKIVRIIFDLARYNRIPVIAEGVES
                     EDVARELIKLGCVQAQGYLYQKPMPFSAWDKSGKLVKE"
BASE COUNT         1803 a         1697 c         1552 g         1743 t
ORIGIN      
        1 cgctcggcgc ttcaacggtt tgttccgccg cgaatgccgt tggggcaaaa aggaataaaa
       61 atgcagaacc cgctgcaaga ccacgaagga gagaggagta ttgcgtcata aatgccaccc
      121 gagtatccat tcaaaaaaaa tacgttatat cgccgtatga taagttcggt gattaataac
      181 gcacacacaa acttaaagaa accgtaagtt ggtaaagttt ttaaagttta tgcaatatcc
      241 tgccgcttcg ctgcttctgg tgtcattttt ttgccagctg ttgcttaaaa gttaacttta
      301 cttcaccatc ggagtcctgt tcagcctgtg gctgcctgtc ggaaaagggg tgagtatgat
      361 tacgctgtgg ggtcggaata attcaactaa cgtaaaaaag tattgctgac gctcgaagaa
      421 ctggaactac cttatgagca aattctcgcg ggccgtgagt ttgggataaa tcacgatgct
      481 gattttctgg cgatgaaccc taacgggctg gtgccgttgt tgcgtgacga cgaaagtgat
      541 ctcattcttt gggaatcaaa cgccattgtc cgctatctgg cggcacagta cgggcaaaaa
      601 cgcctgtgga tcgactcacc ggcacgtcgt gcggaagcgg aaaaatggat ggactgggca
      661 aaccagacgc tcagtaatgc tcatcgcggg atcctgatgg gattagtcag aacaccaccg
      721 gaagagcgcg atcaggccgc cattgatgcc agttgcaaag agtgcgacgc cctgtttgcc
      781 ctgctcgatg cggaactggc aaaagtaaaa tggttctctg gcgacgagtt tggtgtgggc
      841 gatatcgcta tcgcaccgtt catctacaat ttgtttaacg ttggcctgac ctggacaccg
      901 cgtccaaatc tgcaacgctg gtatcagcaa ctcactgaac gccccgcagt gcgcaaagtg
      961 gtgatgattc ccgttagcta attgcgtggg ctaactttaa gtaattcccc actggactcg
     1021 tcggtgagaa cgtataaata accgtcgggt ccagtgcgaa catcacgaat tcgctgccct
     1081 ctgtccgtta aaatacggcc atcttctgtc actttgtcgc cgttgacgct catcacaatg
     1141 acatctttat ctttcagcgc gccaataaat aatttttgct gccactgggg gaatttatcg
     1201 ctgttataga aggccatgcc gctcacagcg ggcgaatctt tccagtaaaa aacaggttgc
     1261 tcggtcccgg cgacgatctc ccctttcgct tccggtatct taaagcctga atagttgatt
     1321 ccccaggttg ccagcggcca gccgtagtgt ttgccttttt gcgggatatt aatttcatca
     1381 ccaccgcgcg ggccatgttc attcagccac agtgcattac tccacggatt catcgccatt
     1441 ccttgcggat tacgaatgcc ataagaccag atctcggcgc gcgcaccgga ttcctttata
     1501 aaaggattat catccgggat ttcgccctgg tcggtcagac gcaccagttt gccctgtaat
     1561 ttatccagat cctgcgccgt cgggcgctga ttgttttcgc ccagagcaat aaaaagataa
     1621 cctttaccgt cgaataccag ccgcccgcca aaatggttgc cggtagacag ttttggcatc
     1681 tggcggaaga cggtgcggaa gtcggtcact tttgagagat catcacttaa gcggccataa
     1741 cccacagcag ttccggcttt gccatcatcg ccaacttcgg aataacttaa ccagatgcgg
     1801 cgagactgag caaaatcagg cgctaaaacc acgtccagca ggccgccctg cccgtgcgcc
     1861 caaacgtccg gaactccgga aagcggcgca gataatcctt ttcctgcttg ccagtgacgc
     1921 aactcgccgc cgcgcagagt gattaacata ccgtgattat cgggtaaaaa ggccagtgcc
     1981 cagggatggt cgagtttgtc ttgcagtact tcgacattta ccgttgcagg agccgcccag
     2041 agagcggaag aaagacaaat aaggggcaca aggaaaaagg attgtcgatg cataacgcct
     2101 ccggattgac tctggcttaa agcgtagtca gtggaggaga taacaaattc atttttacaa
     2161 aaacttaaac atgaaggggg agacgctttc tcccccttag ttttcaggcc ttctcaagca
     2221 tggcgtgctt ctgcaggctc tggatactca gcgttaagct catcagacaa ttttcaagct
     2281 tatcggcgtt gacggtaata acagtcgggc aatcatggtg cccactcatc aaacatactg
     2341 cggctgtcgc taatgcttct tcagcatgat gaagagcact ccactcttcc tgatccagat
     2401 gaagattcaa ccgcagcgat ttatcgtgca gttcgcgatt cagtttaaaa aagttatctc
     2461 gtagatgatt gctttcgctg acggacatgt atccttttgc cttcctcagt tgcacgtatg
     2521 cagcgaggtc aacgcgtgcg tcgatcaacc ggttcagcag atcgattcgc tgtctgtcaa
     2581 cgaacatact tcgatcctcc tctttccagc cttgttgcga ttaaatagta tggctatttt
     2641 ttgagcaaaa tgctgtccag gatcaattct tcacgccaaa ttaacaaaat ttacatttcc
     2701 ttcgcctccg ccagcgaatg agattgcgat acaaatttta tccagaactc tgtagaatcc
     2761 ctgccctgac ctttcctgta actgaatatt ttttaagcta tgagcaaagt aactccccag
     2821 ccgaaaatcg gctttgtttc ccttggctgt ccgaaaaacc ttgtcgattc agagcgtatt
     2881 ctcaccgaac tacgcactga aggttatgac gtggtaccga gctatgacga tgcggacatg
     2941 gtgatcgtca acacctgcgg ctttattgac agcgcggtac aagaatcact ggaagccatt
     3001 ggtgaagcgt tgaatgaaaa cggcaaggta attgtgaccg gttgtctggg ggcaaaagaa
     3061 gatcagatcc gcgaagtcca cccgaaagtg ctggaaatca ccgggcctca tagctatgag
     3121 caggttctgg agcacgttca tcactacgtg ccaaaaccga aacacaaccc attcctgagc
     3181 ctggtgccag aacaaggtgt gaaactgaca ccgcgtcatt atgcctatct gaaaatttcg
     3241 gaaggctgta atcaccgctg caccttctgc attattccgt ctatgcgcgg cgacctggtg
     3301 agccgtccga ttggcgaagt gttaagtgaa gcgaaacgtc tggtagatgc gggcgttaaa
     3361 gagattctgg tgatctcgca ggatacttcc gcctatggcg ttgatgttaa acatcgtact
     3421 ggcttccaca acggcgagcc ggtaaaaacc agcatggtca gcctgtgcga acagttatcg
     3481 aaactgggga tctggacacg tctgcactac gtttaccctt atccgcatgt ggacgacgtc
     3541 atcccactga tggcagaagg caaaatcctg ccgtatctgg acattccgtt gcagcacgcc
     3601 agcccgcgca ttctcaaact gatgaagcgt ccgggttctg tagatcgcca actggcgcgc
     3661 atcaaacagt ggcgcgaaat ctgcccggaa ctgaccctac gctcaacctt tattgtcggc
     3721 ttccctagcg agacggaaga agatttccag atgctactcg acttcctgaa agaagcgcgt
     3781 ctggatcgcg ttggctgctt taaatacagc ccggttgaag gtgcagacgc caatgccctg
     3841 cctgaccagg ttccggaaga agtgaaagaa gaacgctgga accgtttcat gcagctgcag
     3901 cagcagattt ccgccgagcg cctgcaagag aaagtgggcc gtgaaattct ggtgattatc
     3961 gacgaagtgg acgaagaagg cgcgattggt cgcagcatgg cagatgcacc ggaaatcgac
     4021 ggcgcggttt atctcaatgg tgaaaccaac gttaagccgg gtgatatcct gcgtgtgaaa
     4081 gtcgagcacg ccgatgagta cgatttgtgg ggtagccggg tttaattaat tcaggccggg
     4141 caaaatcatt tgtccgccct gcgaataatg attactgcca gctaatctga gccctgccgt
     4201 ttttcttact cttatacagc cgctcatccg ccagcatttt caagcgttcg acggtggtac
     4261 attcatttga atgcgctaca ccgatactgg tacttaaatt aatgcttccc acctggaaat
     4321 gaacgatttc ttttagaatc gatagcaact cttccagcgt tccacccgta taaatcaccg
     4381 caaattcgtc accgccgaag cggtagattt tacctttatc taccgggatc tgtgatttaa
     4441 ggattttcac cactttcatc aacacttcat cgcccagcaa atgaccgaag gtgtcgttaa
     4501 tgtttttgaa tttatcgacg tcaaacaggc acaaataacc gcctttttcc ggcagcgctt
     4561 ttaaatcttc gtcaaaggat tttctgccca ttgctttggt caggtcgtcc aggaacgccg
     4621 agttatacat actgtagagt tttttgacat cggcagccga aaagaaaagc ccgccatcca
     4681 gtacgcgttc aaaatggtta ttaatgatgg cttctttctc cacaatacca cgattaatca
     4741 ctctgcgtac cagatagagg caactggtac ccgatatcac tgccaccaat gcggcaaaga
     4801 aaataatcaa tgtacagacc cgcaaataaa catctttttt atacgcaaat acgtacaatc
     4861 cggtcggcaa ataggcgaag tgctccatcg cttccgaaac ggtgaagatg gcgtttttag
     4921 cattatattc atcaccggtt taccgttctt aaatagagag aacatcaggt tctcgctgtc
     4981 catcgccagc gattttgaca gtaaagaaaa acgctccaga ccaagcaaaa atatcagact
     5041 acgattgtga gttaccggat agaggtaata gacgaaggtt ttaccgtcaa aagagataaa
     5101 ccctgtattt ttgacatgtc gatacttcac aacctgcata atatctgcac aataattttt
     5161 ctcgaactcc tttacctgaa tacattcgtc actggaaatc agataaggac cgacatcgta
     5221 caaataagag ggggaaaccg agtcggcgta agctgtgcgc aaaccaacca acagctgttc
     5281 ctccgtggga tttagcgtgt tgataagatc aattgccacg ctacgccgat attcagcaat
     5341 atttgccacc gcaaaagcag tatcaataac ggactgtttc aggctataca cccgttcctt
     5401 taccatttga gtgtagatcc cgcaggcaac tgctgaaatc atgataaaaa tcagcagact
     5461 gactgtaagt acgaacttat tgattctgga catacgtaaa ctactctttt actaattttc
     5521 cacttttatc ccaagcggag aatggcatgg gtttctggta cagatacccc tgagcctgaa
     5581 cacatcctaa tttaattaat tcgcgcgcaa cgtcttcgct ctctacgcct tccgcaatca
     5641 ccgggatccg gttatagcgt gccagatcga aaataatgcg cacaattttt cccgcagtac
     5701 cgaaaatatc gccggtaaat tgcgagtcca cctttatgct gtctggatta atttcataaa
     5761 aacgtttcag gtttgacaaa ccggtgccaa agtcatcaat cgatatccgt accccttgtt
     5821 tacgcagttg ttcaatattg agataaaaat gttcattaat tcgctcgatc acggtttcag
     5881 tgatctcaac acagaggctg ttcgccttca ggccattttc tttcatgatg gtggtaaaac
     5941 gctcatgaaa gtgcggttcg tttaactgcc agggcgaaac attaatatgc agtaagaaat
     6001 catccttcag cgacgcacgc tgactaaaat gtctgaattc acccattgcc gttttaataa
     6061 tctgataacc cagctcattg ataaacccga tatcttcagc aatggtaatg aacttcagcg
     6121 gtgaaatgat cccccgctcc gcagaaaccc aacggcacaa cgcctccgcg cccagaatgg
     6181 cgcgggtgtt aatatcaacg atcggttgat agacgaggaa tagttcgcca atttccattg
     6241 catggcgaat attacgcgca atccagatat tctcttctat ttcgttgagt ttcgcgtcat
     6301 cacagaaaat aaactcggag aacatatggt cacggatact ggaaatcgcc agtttcagtt
     6361 tttctgcgta actccaggcg ttttccccct gcaaaggttc acaaatggca gacttatgga
     6421 tgcgcagatt gcggtagcca aagctgctgt caaaatcgtt taccatcgct attttacggg
     6481 taaatgtctg tacattttcc cgacacagaa gtaaatagag atcggcattg aatgtcacga
     6541 tatcgcagca ttgcgcatat ttctcggcaa ttcgctgaca caatttcctg acgattttat
     6601 tgcgttcatc aacaccaaac accgcttcca cggcttccag accacacagt ttgatgatcc
     6661 ccacagccag atactgatta ttgttggact caataatcgc catattttta ataaaaccgc
     6721 tttcctgatc gtaagagatc agtgagagca atttatttga ccgttcaatt tcggcatctt
     6781 tcatttccgc gatcg
//
LOCUS       DAJTZL010000193         6762 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.194, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000193 DAJTZL010000000
VERSION     DAJTZL010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6762
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.194"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(61..204)
                     /locus_tag="OO544_003595"
     CDS             complement(61..204)
                     /locus_tag="OO544_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_072020761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="HCR6769105.1"
                     /translation="MKKITGIILLLLAAIILAACQANYIRDVQGGTVSPSSSAELTGL
                     ATQ"
     gene            complement(302..553)
                     /locus_tag="OO544_003596"
     CDS             complement(302..553)
                     /locus_tag="OO544_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012644888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E5 immunity protein"
                     /protein_id="HCR6769106.1"
                     /translation="MKLSPKAAIEVCNEAAKKGLWILGIDGGHWLNPGFRIDSSASWT
                     YDMPEEYKSKIPENNRLAIENIKDDIENGYTAFIITLKM"
     gene            complement(621..2291)
                     /locus_tag="OO544_003597"
     CDS             complement(621..2291)
                     /locus_tag="OO544_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-type pyocin domain-containing protein"
                     /protein_id="HCR6769107.1"
                     /translation="MSGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENN
                     PWGGGSGSGIHWGGGSGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGG
                     LAVSISAGALSAAIADIMAALKGPFKFGLWGVALYGVLPSQIAKDDPNMMSKIVTSLP
                     ADDITESPVSSLPLDKATVNVNVRVVDDVKDERQNISVVSGVPMSVPVVDAKPTERPG
                     VFTASIPGAPVLNISVNNSTPAVQTLSPGVTNNTDKDVRPAGFTQGGNTRDAVIRFPK
                     DSGHNAVYVSVSDVLSPDQVKQRQDEENRRQQEWDATHPVEAAERNYERARAELNQAN
                     EDVARNQERQAKAVQVYNSRKSELDAANKTLADAIAEIKQFERFAHDPMAGGHRMWQM
                     AGLKAQRAQTDVNNKQAAFDAAAKEKADADAALSTAMESRKKKEDNKRDAEGKLNDEL
                     AKNKGKIPDLKIDQKIRGQMPERGWTEDDIKNTVSNGATGTSFDKRSPKKTPPDYLGR
                     NDPATVYGSPGKYVVVNDRTGEVTQISDKTDPGWVDDSRIQWGNKNDQ"
     gene            complement(2524..4743)
                     /locus_tag="OO544_003598"
     CDS             complement(2524..4743)
                     /locus_tag="OO544_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HCR6769108.1"
                     /translation="MAIYHLSMKIISRKNGYSAVASAAYRSGSVIPDDRTGLTHDYTR
                     KRGVDDAVILTPANAPSWCGDRSVLWNAVEKAEQRRNSQLAREIELAIPREISREAAR
                     ETVLAFVRENFVSRGMIADVAFHHMDRTNPHAHIMLTTRAVGETGFAGKVRDWNDRAL
                     AETWRASWADHANRALANAGYQEEIDHRSYERQGLEKAPGLHLGKAACAMEKRGMETE
                     RGEQNRLINSLNLEIQVSRTRLAVIQVQENVRKRELSDAARRAAEALNLTIPAANASA
                     DTLREFIATLPQECGNAWEMTPEFLAMSGKVNDIEREGNALLKEQAILEKEMTGLKKA
                     RPVASLLSEIPLMTWAEPEYRKRQLRFWKLGKQIESLRRTYRAVKERDIPARRQAFET
                     QWNTWIAPGMAELKEKLSAREAERRREEAEAEARRKEQEHEARLKRHDNHRLSRETAL
                     VGVITELGRAREPGTGRITRYMMLSNRAGEFTVWGDELAHYPQSVHDPVNVYLSPGGA
                     VMVSDIREGMPESHETMARPERVRMYSGATVRHVLEQMRQGWPSYGFPALPHHWPDNF
                     YFSDDRRPVASPLPSAHRVDITADAVPEQLMPVVFSREGNSRTLNLLLCKGPEEMLVG
                     FVRREGGFCPVLVLPSPDYSHLIVSTITENGVCLAGYGEAINHDEDTPYPPEPHLMQF
                     RLKGHHDRLLAAVHKPEEMPDYLFRQLGFNQTWHEWKRDEQHRQQQRRPGHFRGMSM"
     gene            4922..5188
                     /locus_tag="OO544_003599"
     CDS             4922..5188
                     /locus_tag="OO544_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="HCR6769109.1"
                     /translation="MSRTLEQKIAEAEARLQRLKAKSRSLDTAQKVIVGAAMLARIRR
                     PEEAQLRAFLLQFLRKEVTRQADVNRIQPLINELEKLPKPPAKP"
     gene            complement(5402..6328)
                     /locus_tag="OO544_003600"
     CDS             complement(5402..6328)
                     /locus_tag="OO544_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HCR6769110.1"
                     /translation="MSAALQYFEENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQ
                     PHAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASV
                     KALKYAAAIERALCEKLGADVNYSGLICKNPFHLEWLVMEWREEAYTLDELADYLDLS
                     ASARRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPVFSQWLDAVIQRVEMYNVSLPV
                     PLSPAECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRKGGKIGGAKSKRG
                     AVATSARTLKPWETLGISRAWYYQLKKRGLVE"
BASE COUNT         1597 a         1889 c         1547 g         1729 t
ORIGIN      
        1 aaaaaaaagc gcgtcagaat cagcctgaca cgctttgttc ttgtcaaaga ggatttcggg
       61 ttactgcgtt gctaatccgg tcagttcagc tgaggatgac ggggatacag tcccgccctg
      121 aacatcgcgg atatagtttg cctgacatgc agcaagaata atggctgcaa gaagcaataa
      181 aataatccct gttatttttt tcatactgtt ccttgtatat atggactcag gcattcatcc
      241 ataaaaacct gaaatccaaa ggttgaaatc atccatgaaa cataaaattc tataacacta
      301 tttacatctt taacgtgata atgaaagcag tgtatccatt ctcaatatca tctttaatat
      361 tttcaatagc caatctatta ttttcaggga tttttgattt gtattcctcc ggcatatcat
      421 atgtccatga tgctgaacta tctatcctga atccaggatt cagccaatgc ccaccatcaa
      481 tgcccaaaat ccataagcct tttttcgctg cttcattaca aacttctatt gcagcttttg
      541 gtgataactt catagaagca tttcctttta attcaaaaaa ggcatcacca ctatcatata
      601 acaccgtatg ttcaaataat ttattggtca tttttatttc cccattgaat tctcgaatcg
      661 tccacccaac ccggatctgt cttatcacta atctgagtaa cctcaccagt acgatcattg
      721 acaacaacat atttaccagg agaaccatac actgttgctg gatcatttcg acctaaatag
      781 tctggaggtg ttttcttcgg actacgctta tcaaaagatg taccagttgc accgttagat
      841 acggtatttt tgatatcatc ctctgtccat cctctttctg gcatttgtcc cctaattttc
      901 tgatctattt ttagatctgg tatctttcct ttattttttg ctaactcatc atttagtttg
      961 ccttcagcat ctcttttatt atcctctttc ttcttcctgc tttccatcgc agtacttaat
     1021 gcagcatcag catctgcctt ctcttttgca gcagcatcaa atgcagcctg cttattattt
     1081 acatccgtct gcgcccgctg ggctttaagc ccggccattt gccacattct gtgaccgcca
     1141 gccattgggt catgtgcaaa tcgttcaaat tgttttattt cagctattgc atcagcaaga
     1201 gttttattcg ctgcatcaag ttcgctttta cgcgaattat aaacctgaac agctttagcc
     1261 tgtcgctcct gatttctggc aacatcttca tttgcctgat tcagctctgc acgcgcgcgt
     1321 tcataatttc gctcagccgc ttcaaccgga tgcgtagcat cccattcctg ctgacggcga
     1381 ttttcttcat cctgacgttg ttttacctgg tcagggctaa gaacatcact cactgaaaca
     1441 tatacggcat tatgaccgct gtccttcggg aatcgaataa ctgcatccct ggtattaccc
     1501 ccctgagtaa atcctgccgg gcgaacatcc ttatcagtat tatttgtaac acctgggctt
     1561 aatgtctgta ctgctggcgt actgttatta actgaaatat tcagaacagg tgcacctgga
     1621 attgatgccg taaaaacacc tggacgttcg gtaggttttg catcaaccac cggaacactc
     1681 atcggaacac ctgaaacaac cgaaatattc tgtcgttcgt cttttacatc atcaacaaca
     1741 cgaacattta cgtttactgt tgccttatcg agaggtaatg aactgacagg tgattcagta
     1801 atatcatctg cgggtaatga cgtcacaatc tttgacatca tattggggtc atctttcgct
     1861 atttgtgatg gcaatacacc atataaagcc accccccaaa gaccaaattt aaacggtcct
     1921 ttcagggcag ccataatatc agcaatagct gccgataatg ctcccgctga aatactgacc
     1981 gccagaccgc cagcacctgg agtggaaagt gccggaaaac caaatgccac tggcgcagct
     2041 actgctgaca gattaccgcc tgttcccgag ccaccaccgg aattaccatt ccccccgcca
     2101 ttaccatgac cggaaccacc tccccagtga atgccgctac cggaaccacc accccacggg
     2161 ttattttccg aactccaccc ggaaccatca gaagcaccac cacctacacc aagcccggtc
     2221 gggccaccat taatgttacc acttgtgcta tgcgcgcccg tgttatggcc gcgtccatcg
     2281 ccaccgctca taaaaattcc ctcttttaag cgttaaaaca attaattaca cgattaatac
     2341 tgtacataaa accactggtt ttatgtacag atcaaaaagt acccgtcatc gttttttctg
     2401 tcaagctcag gtggaaaatt ttttagaaat caggagggat aacacttaga aaacgcagag
     2461 cgggccacaa aaaattttgt ggcccgtgat gtgttggcgg ggtccggggt gcagccccgg
     2521 acatcacata ctcatgcccc ggaagtgccc ggggcggcgt tgttgttgcc tgtgctgttc
     2581 gtcccgcttc cactcatgcc aggtctgatt aaaaccgagt tgacggaaga gataatccgg
     2641 catctcttcc ggtttgtgga cagcagccaa aagcctgtca tgatggcctt tgagccggaa
     2701 ctgcatcagg tgcggttccg gtgggtacgg agtatcctca tcatggttta tagcttctcc
     2761 gtaacctgcc aggcataccc cgttctccgt gatggtgctg actatcagat gactgtaatc
     2821 cggcgacgga agcacaagaa cggggcaaaa ccctccttcc cggcgaacaa acccgacaag
     2881 catttcctcc ggccctttgc ataacaacag attcagcgtc ctgctgtttc cctctctcga
     2941 aaatacaaca ggcatgagtt gttccggtac agcatcagcg gtgatatcca cccggtgcgc
     3001 tgacggcagc ggagaggcta cgggcctgcg gtcgtcgctg aaataaaaat tatccggcca
     3061 gtgatgcggc agcgccggaa aaccgtaaga gggccacccc tggcgcatct gttccagtac
     3121 atgccggacc gtcgcaccgg aatacattct cacacgctca ggccgcgcca tcgtctcatg
     3181 agattctggc attccctcac gtatatccga gaccatcaca gccccgcctg gcgacaggta
     3241 aacattcacc gggtcatgaa cactctgggg gtaatgcgcc agctcatcac cccataccgt
     3301 gaattctccg gctctgttac tcagcatcat gtagcgggtt atcctgcccg ttcccggctc
     3361 tctggcacgc cccagctccg taataacccc gactaatgcc gtttcacggc tcagacggtg
     3421 gttatcatga cgtttcagcc gcgcctcatg ctcctgttcc ttccggcgcg cttccgcttc
     3481 ggcttcctcc ctgcgccgct ccgcttcccg tgctgacagt ttttctttca gctctgccat
     3541 tcccggcgca atccacgtat tccactgcgt ttcaaaggcc tgacgacggg cgggaatgtc
     3601 ccgttctttc acggccctgt aggtgcggcg aagagattca atctgtttcc cgagtttcca
     3661 gaaacggagt tgtcttttgc ggtattccgg ttcagcccat gtcatcaggg ggatctctga
     3721 cagaagggaa gcgacagggc gtgctttttt gagtccggtc atctcctttt cgagaatggc
     3781 ctgctctttc agcagcgcat tcccctcacg ttcgatgtcg ttcaccttcc cgctcatcgc
     3841 caggaactcc ggggtcatct cccacgcgtt gccgcattcc tgcggcaacg tggcaatgaa
     3901 ttcccgcagg gtatccgctg aggcattcgc agcgggaatg gtcaggttaa gggcttccgc
     3961 tgcacgacgt gcggcatcgc tgagttcccg tttccgcacg ttctcctgta cctggatgac
     4021 tgccagcctt gtgcgggaaa cctgtatttc caggttaagg ctgttaatca gacggttctg
     4081 ctcaccgcgt tctgtttcca ttccccgttt ttccatcgca caggcagcct ttccgaggtg
     4141 aaggccgggc gctttctcca gtccctgacg ctcgtatgaa cgatggtcta tctcttcctg
     4201 gtagccggcg ttcgcaagcg ctctgttcgc atggtcagcc catgatgcgc gccacgtctc
     4261 ggcgagtgcc cggtcgttcc agtccctgac ctttcctgca aatcccgttt ccccgacagc
     4321 tctcgtggtc agcatgatgt gcgcatgggg attggtccgg tccatgtgat ggaacgccac
     4381 atcggcaatc atgccccgac tgacaaagtt ttcccggaca aaagcgagaa cggtctcccg
     4441 tgcggcctcg cgggaaatct cacggggaat ggcgagttca atctcccttg ccagctggga
     4501 gttccggcgc tgttcggctt tctcgaccgc attccagaga acggaacggt caccacacca
     4561 ggacggtgca ttcgcagggg tgagaatgac cgcatcatca acgccgcgtt tacgggtgta
     4621 atcgtgggtt aatccggtac ggtcatcggg tatgacagag ccggaacggt aggcagcaga
     4681 agcaacggca ctgtagccgt tttttcgcga aatgattttc atgctgagat gataaatcgc
     4741 catttctgcc gtaatgcctt tttgccgttt tcttttagcg tcgcagaccg cacacaatcg
     4801 aaacgaagtg gagattgtat aagtgcgcac ttcgtgatgt gccgtgcatt atgccagttc
     4861 agttttgcaa ctaccagtga cgcgctaaaa tgcgattttt caccgcgcat ggagtcacaa
     4921 aatgagcaga acactcgaac agaagattgc cgaagctgag gccagattac agcgcctgaa
     4981 ggcgaagagc cggagcctgg acacggcgca aaaagtcatt gtgggggctg ccatgctggc
     5041 gaggatacgg aggccggaag aggcgcaact gcgtgcgttc ctgctccagt tcctgcgaaa
     5101 ggaagtcaca agacaggccg atgtgaaccg gatacaaccg ctgattaacg agctggagaa
     5161 gctacccaaa ccacctgcga aaccgtagaa cggagaaacc ccggcgcgta agtgccacat
     5221 gcaccaccga cacctttgcg cttcctcgct cattcgctcg ctacgctcgg tcatgagact
     5281 gcggcgagcg ctacaacctc aatcatgcgg aaagccgcac cattgcaggg aggaaacgaa
     5341 aaagccacca aacgccaaca gctcaaaaaa gcgctgttat ctgatatagg ttttatttgg
     5401 tctactctac aagacctcgt tttttcagtt ggtaatacca ggcgcggcta attccaagag
     5461 tctcccacgg tttcaacgtt cgcgcagatg tggcaacagc accacgctta gacttagcac
     5521 ctccaatttt cccgcccttg cgaccacgtg cagcctgaat ttctggcgtg tgcgtatcag
     5581 ccacatactg tgcgaaagtt tccggcgtga agttcctgtg cgtgtatttc gcaatgctct
     5641 tgccaatagc ccgacattcc gccggagaaa gcggaacggg aagcgatacg ttgtacattt
     5701 cgacacgctg gatcacggca tcaagccatt gtgagaatac aggccagccc tgacgaatcg
     5761 ccctgtaagc ccatttgcgc gtcatttcga acaggtggca gtttcgcccc atcccgtaat
     5821 gtttatcgat gctacggcgc gctgaggcgc tcaaatcgag ataatcagcc agttcatcga
     5881 gggtataggc ttcctcgcgc cactccatca ccagccattc caggtggaac ggatttttgc
     5941 aaatcagtcc gctgtagttc acatctgcac caagtttctc acacagcgca cgctctatcg
     6001 cggcagcgta cttcagtgcc ttaaccgacg catcaggcgc ggttctcacg gcgatgttga
     6061 gtgcatagag caggtgagca tgaccgttca cgggattttt tacggtgatg ttcggcgcgg
     6121 gggcgttccg gtcgctccag tcaatcgcag ccccctcgcg gtcaacgtca aaaaccagcc
     6181 agaactgcgc atgaggctgg ttctgctgga tgtaccgcgc aagaagcgca cgccctttgc
     6241 cggagatgcg aagaccaaaa gcgagatcat ccgtgtgata gggacggtgg ggtaaatttt
     6301 cttcgaagta ttgaagcgcg gcgctcatgt gttacgattc cttccaggtt atcgaccgac
     6361 ctcgcccctg tattctcgtg acaatcttgg cggagagaca cgaatttcag gggcgtttgt
     6421 ttttcagtcg cttacactac acccaacggc aaatcctgtc gaaccttccc gaaaacccga
     6481 tccatttacc ataactcccg ttgtacgtac cccttaaaac gccctcaatc gctttctgac
     6541 gcaaaaaacg caaaaagaca aggaaacaca gggtaaacat aaaaaaatgc gtcagaatcg
     6601 cttttagcgc gttgcataac tatgcacgaa agcgatgcgc gatcggtttc gtgcgttctg
     6661 aacaccgaac ggctcaaaac gacaagtttt ctccccctgg atggaaagtt ctgcacgagg
     6721 attttagatc ccatctccca catggaattt ttgtgacact tc
//
LOCUS       DAJTZL010000194         6694 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.195, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000194 DAJTZL010000000
VERSION     DAJTZL010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6694
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.195"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            375..1250
                     /locus_tag="OO544_003601"
     CDS             375..1250
                     /locus_tag="OO544_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HCR6769111.1"
                     /translation="MRKFILISMITLASVSLGACRQNVTIKQDAPGQKAFLTDGQIYD
                     LHSGKIISSSELLADLATARHLIIGEKHDNAEHHQIELWLIQNLLIQRPQGSVLLEML
                     TSEQQPRVNQVKCWLKDNPVVRDSRVQELLNWQKGWSWEMYGDIVMQLLRGPYPLLNA
                     NIGREQMLALYKKNEFPKGKKSTAPVVQEALRETIISMHEGNLESQQLTSMLSIQQQR
                     DRYMARQLLSAPVPSLLIAGGYHASKSMGVPLHMEDLATGTHPVVLMLAEKGMNITVD
                     HADYVWFVAPDTTKR"
     gene            1258..2034
                     /locus_tag="OO544_003602"
     CDS             1258..2034
                     /locus_tag="OO544_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000981087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HCR6769112.1"
                     /translation="MMNILHFSQSVKWSSWFICSLLLHGLIFLAFIWRFSEVQPAMSP
                     APAIMLQWAEIIEAPSSPLSLPVGIAQQESAVTEEKQQTEDRQQRPVTEDSDATIEIT
                     RKKKSSDGEKKKTRPPRKIKAQTSDSNPTAVSSNAAPQALVESSRIAAPFNSDSTKRD
                     NSEASWESRVKGHLNRYKRYPGDARKRARTGTAVVTFTVNTEGTIVSSFLEISSGTLS
                     LDREAIAVLERAQPLPKPPPEILEGGLFKVKMPITFKLKE"
     gene            2203..4464
                     /locus_tag="OO544_003603"
     CDS             2203..4464
                     /locus_tag="OO544_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HCR6769113.1"
                     /translation="MNVIKLAIGSGILLLSCGAYSQSISEKTNSDKKGAAEFSPLSVS
                     VGKTTSEQEALEKTGATSSRTTDKNLQSLDATVRSMPGTYTQIDPGQGAISVNIRGMS
                     GFGRVNTMVDGITQSFYGTSTSGTTTHGSTNNMAGVLIDPNLLVAVDVTRGDSSGSEG
                     INALAGSANMRTIGVDDVIFNGNTYGLRSRFSVGSNGLGRSGMIALGGKSDAFTDTGS
                     IGVMAAVSGSSVYSNFSNGSGINSKEFGYDKYMKQNPKSQLYKMDIRPDEFNSFELSA
                     RTYENKFTRRDITSDDYYIKYHYTPFSELIDFNVTASTSRGNQKYRDGSLYTFYKTSA
                     QNRSDALDINNTSRFTVADNDLEFMLGSKLMRTRYDRTIHSAAGDPKANQESIENNPF
                     APSGQQDISALYTGLKVTRGIWEADFNLNYTRNRITGYKPACDSRVICVPQGSYDIDD
                     KEGGFNPSVQLSAQVTPWLQPFIGYSKSMRAPNIQEMFFSNSGGASMNPFLKPERAET
                     WQAGFNIDTRDLLVEQDALRFKALAYRSRIQNYIYSESYLVCSGGRKCSMPEVIGNDW
                     EGISDEYSDNMYIYVNSASDVIAKGFELEMDYDAGFAFGRLSFSQQQTDQPTSIASTY
                     FGAGDMTELPRKYMTLDTGVRFFDNALTLGTIIKYTGKARRLSPDFEQDEHTGAIIKQ
                     DLPQIPTIIDLYGTYEYNRNLTLKLSVQNLMNRDYSEALNKLNMMPGLGDETHPANSA
                     RGRTWIFGGDIRF"
     gene            4533..5708
                     /gene="senB"
                     /locus_tag="OO544_003604"
     CDS             4533..5708
                     /gene="senB"
                     /locus_tag="OO544_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterotoxin production-related protein TieB"
                     /protein_id="HCR6769114.1"
                     /translation="MNIFTLSKAPLYLLISLFLPTMAMAIDPPERELSRFALKTNYLQ
                     SPDEGVYELAFDNASKKVFAAVTDRVNREANKGYLYSFNSDSLKVENKYTMPYRAFSL
                     AINQDKHQLYIGHTQSASLRISMFDTPTGKLVRTSDRLSFKAANAADSRFEHFRHMVY
                     SQDSDTLFVSYSNMLKTAEGMKPLHKLLMLDGTTLALKGEVKDAYKGTAYGLTMDEKT
                     QKIYVGGRDYINEIDAKNQTLLRTIPLKDPRPQITSVQNLAVDSASDRAFVVVFDHDD
                     RSGTKDGLYIFDLRDGKQLGYVHTGAGANAVKYNPKYNELYVTNFTSGTISVVDATKY
                     SITREFNMPVYPNQMVLSDDMDTLYIGIKEGFNRDWDPDVFVEGAKERILSIDLKKS"
     gene            complement(6234..>6694)
                     /locus_tag="OO544_003605"
     CDS             complement(6234..>6694)
                     /locus_tag="OO544_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HCR6769115.1"
                     /translation="QNGLAKALREIGRIERTLFMLDWFRDPALRRRVQAGLNKGEARN
                     ALARAVFMHRLGEIRDRKPENQSYRASGLTLLTAAISLWNTVYMERAVDALKRKGMKI
                     NAQLLSHLSPLGWEHINLTGDYIWKNSRIPTSGKFRRLRSVKIDKVKKQP"
BASE COUNT         1894 a         1550 c         1536 g         1714 t
ORIGIN      
        1 ttaacgtacg ttttcgttcc attggccctc accccccgat aacgacccag gtagcgcgcc
       61 gtattccccc cgccggccgt tttcttcgac atgtacatca ggcgcggaac tcatcggggc
      121 gcgggaatga aatcccattc agactccgga tagattcaag caagcccact tatcgtcaaa
      181 atcggtgttg caaaaacggg agtgaccata gatccgtttt ccaagcggac cccataatat
      241 cggctggtaa acatcactcg ctagtgcatt ctttttgttg gtaatgataa tcatttacat
      301 tcattctttg atgttttatc ataattaatg ttttgtcaat aaatatatgg aaacatattg
      361 tttcagatgg gaatatgaga aagttcatcc ttatttctat gataacgtta gctagtgtta
      421 gtctgggagc ctgcagacag aatgtcacca taaagcagga tgcgccaggg cagaaagctt
      481 tcctgactga cgggcagatt tacgatttgc acagtggcaa aattatttct agcagcgagc
      541 ttttggcgga tcttgctacc gctcgacatc ttattattgg tgaaaaacac gataatgccg
      601 agcatcatca gattgagctt tggcttattc aaaatttgct catccagcga ccacagggga
      661 gtgtgttgct tgagatgttg acgtcagagc aacagccccg agttaaccaa gttaaatgtt
      721 ggctgaaaga taatcctgtg gttcgagata gccgtgttca ggaactactt aactggcaaa
      781 aagggtggtc ctgggagatg tatggcgaca tcgtcatgca gctattacgt gggccttacc
      841 cgctgctcaa tgccaacatt ggccgggagc agatgttggc actgtacaaa aaaaacgaat
      901 ttcctaaagg aaaaaaatcc actgcacctg tggtacaaga agctctgcgt gaaaccatca
      961 tatctatgca tgaaggcaac cttgaaagcc agcaactgac gtcaatgctc tctattcagc
     1021 agcagcggga tcgctacatg gcccgccagc ttctgagtgc cccggtaccg tcgttgttga
     1081 ttgcaggtgg ttatcacgcg tcaaaaagca tgggcgtacc gttacacatg gaagatcttg
     1141 ccaccggcac ccatccagtg gtgttgatgc ttgcagaaaa aggaatgaac atcacagtag
     1201 atcatgctga ctacgtgtgg tttgtagcgc cagatacaac aaaaaggtaa accatggatg
     1261 atgaatattc tccacttctc acagtcggta aaatggagca gctggtttat ctgttctcta
     1321 ctgcttcatg gactgatttt ccttgcgttt atctggcgtt tttccgaagt tcagcccgct
     1381 atgtctcctg caccggcgat catgctgcaa tgggcagaaa ttatagaggc tccttcttct
     1441 ccactttcgc ttccggtggg aatcgctcaa caggagtccg cagtgacaga agaaaagcaa
     1501 caaactgagg acagacaaca acgtcctgtc actgaagaca gtgatgcaac cattgaaata
     1561 actaggaaga aaaagtcatc cgatggagaa aagaaaaaga cacgtccacc tcgtaagata
     1621 aaggctcaaa ccagcgacag caaccccaca gccgtttcca gtaatgctgc acctcaggct
     1681 ctggtcgaat cgtcccgtat tgctgcgcct tttaacagtg actcaacaaa gcgagataat
     1741 tctgaggcat cttgggaaag cagagtaaaa ggccacctaa accgttataa acgttaccct
     1801 ggcgatgcca gaaaacgtgc acgaaccggc acagccgtcg tgacttttac cgtaaacacg
     1861 gaaggcacta ttgtcagtag tttcttggaa atctcttcag gaaccctttc tcttgaccgc
     1921 gaagctattg ctgttttaga acgcgctcaa ccacttccca aaccaccgcc tgaaatattg
     1981 gagggaggat tatttaaagt aaaaatgccg attaccttta agttgaagga gtaaagggat
     2041 aattggccgt tcaagctcat ttaactgaaa tttaattttc atttttatgc tgaacatttt
     2101 atcaaacttt tccactgtcg aaaaaatcaa aaagacagta gaacataatt aataaacaaa
     2161 tagttatact tatctttatt aatattatac ccaaggaaat ttatgaacgt tataaaactg
     2221 gctatcggct caggcatatt attgctcagc tgcggtgctt actcacaatc catcagtgaa
     2281 aaaactaatt ccgacaaaaa aggagcggca gaattcagtc cgctcagcgt ttctgtcggg
     2341 aagacgacca gtgagcagga agctctcgag aaaacaggcg cgaccagttc ccggacaacg
     2401 gacaaaaacc tgcaatcact tgacgcaaca gtgcgtagta tgcctggtac ttatactcaa
     2461 atagatcctg gtcagggagc aatcagtgtg aatattcgag gcatgagcgg atttggtcgt
     2521 gtaaacacta tggtcgatgg tattacccag agtttttacg gaacctctac ctccggaaca
     2581 acgacgcatg gttcaactaa caatatggct ggcgtactta tagatcctaa cttactggta
     2641 gcagttgatg ttacacgcgg tgacagcagt ggctctgaag ggatcaacgc ccttgccggt
     2701 agtgcaaata tgcgtactat tggcgttgac gatgtaatat ttaacggtaa tacatatggc
     2761 cttcgttcac gtttctctgt cggtagtaat gggctgggac gcagcggaat gatcgccctt
     2821 ggtggaaaaa gcgacgcttt tacggatacg ggaagcattg gcgttatggc tgctgtgagc
     2881 ggcagttctg tgtactctaa tttctcaaat ggttctggaa ttaacagcaa agagtttggt
     2941 tatgataaat atatgaagca gaaccccaaa tcccaactgt ataaaatgga tatcagacca
     3001 gacgaattta acagcttcga actttccgct cgaacctatg aaaataaatt tacacgtcgt
     3061 gatataacca gtgacgacta ttacattaaa tatcattaca cccctttttc tgaattaatt
     3121 gactttaacg taacggccag taccagtcgc ggtaatcaaa agtatcgtga tggctcgctg
     3181 tatactttct acaaaacctc agcgcaaaat cgttctgacg cgctggatat caacaatacc
     3241 agccggttca ctgtcgcgga caatgacctg gagtttatgc tgggcagcaa actgatgcgt
     3301 acccgctatg accggaccat tcactcagcg gcgggcgacc cgaaagcgaa tcaggaatcg
     3361 atcgagaaca atccgttcgc accctccggc cagcaggata tttcagcgct gtataccggg
     3421 ctgaaggtta cgcgcggcat ctgggaggca gatttcaatc tcaactacac acgtaacagg
     3481 atcacagggt acaagcccgc ctgcgattca cgcgttatct gcgtgccaca gggtagctac
     3541 gatattgacg ataaagaggg tggcttcaac ccttcagttc agctttctgc tcaggtaaca
     3601 ccatggcttc agccgttcat tggctacagc aaatccatgc gcgccccgaa catccaggag
     3661 atgttcttct ctaattcagg aggcgcatcc atgaacccat tcctgaagcc tgaacgtgca
     3721 gaaacctggc aggcgggttt taacattgat accagagatt tactggtcga acaggatgcc
     3781 ctgcgcttta aggctctggc gtaccgcagc aggatccaga actacatcta cagcgagtct
     3841 tatctggttt gttctggagg tcgtaaatgc agtatgcctg aggtgattgg caatgactgg
     3901 gagggcatta gcgatgaata cagcgacaat atgtacatct acgttaactc ggcaagcgac
     3961 gttatcgcaa agggcttcga actggagatg gattatgatg caggttttgc ttttggccga
     4021 ctctctttca gccagcagca aacagaccag ccaacctcca tcgccagcac ctactttggc
     4081 gcaggggata tgaccgaact gcccagaaaa tacatgacgc tggatactgg tgttcgcttc
     4141 ttcgataacg cgttgaccct gggcactatc ataaaataca caggcaaggc tcgtcgcctg
     4201 tcgcctgatt ttgagcagga cgaacatacc ggcgcaataa tcaaacagga tttgccgcag
     4261 atcccaacga ttatcgatct ctatggtact tacgagtaca accgcaacct aacactgaaa
     4321 ctttcggtac aaaacctgat gaacagagat tattcggagg cgctgaataa gctcaacatg
     4381 atgccaggtc ttggtgacga gacccaccca gccaattccg cgcgtggcag aacatggata
     4441 tttggcgggg acattcgttt ctgatgacaa tttccgctag gttcagacaa tacgttttct
     4501 ctcttatgtc aattttattg caggaacgaa aaatgaatat tttcacttta tccaaagcac
     4561 cgctatacct gttaatttca ctatttttac ccacgatggc catggctatc gatccacctg
     4621 aacgcgaact ttcgcgattt gccctgaaaa cgaattacct tcagtcccct gatgaaggcg
     4681 tctatgaact ggcgtttgat aatgccagta aaaaggtgtt tgcagcagtc accgatcgtg
     4741 taaatcgtga agccaataaa ggctatctgt attcgtttaa ttcagattcg ctgaaagtcg
     4801 aaaataaata cacgatgcca taccgggcat tttcgctggc gataaatcag gataaacatc
     4861 agctctatat cggacacacc cagtcagcgt ccctgcgtat cagtatgttt gacaccccaa
     4921 ccggcaaact ggtaagaacc agcgacaggt taagttttaa agcggcaaac gctgcagatt
     4981 cgcgttttga gcattttcgc catatggttt acagccagga ttccgatacc ctgtttgtga
     5041 gttatagcaa tatgctgaaa acggccgagg gcatgaagcc tctgcataag ctgttaatgc
     5101 tcgacgggac gacgcttgcc ttaaaaggcg aggttaagga tgcttacaaa ggtacagcgt
     5161 atggtctgac gatggatgaa aaaacacaga aaatctacgt tggcggaaga gattacatca
     5221 acgaaattga tgcgaaaaat cagacgctgc tgcgtaccat cccgttgaaa gatccgagac
     5281 cacaaatcac aagtgtgcag aatctggcgg tggactccgc ttctgaccgt gcctttgtgg
     5341 tggtattcga ccatgacgat cgttccggta caaaagatgg actctatatt tttgacttac
     5401 gcgacggtaa acagcttggc tatgtgcaca caggagccgg agctaacgcg gtgaaataca
     5461 atccgaaata taacgaactg tatgtcacca acttcactag cggcaccatc agcgtagtgg
     5521 atgccaccaa atacagcatc acccgtgaat ttaacatgcc ggtctaccca aaccagatgg
     5581 tgttgtcgga cgatatggat accctttaca ttggcatcaa agaaggcttt aaccgcgatt
     5641 gggatcctga tgtgtttgtg gaaggagcta aagaacgtat tctgagcatt gatttgaaaa
     5701 agtcgtgatc ggggggaaaa aacatgggat aaaaactccg ttattccact aaactggccg
     5761 gactaggaaa agcaaacatc tctgtcctgc cggtacgcct gttacgccgc tccatgaggc
     5821 tgaggaatct ccagaaaacg agacaggcac agcattgtac gatcaactga tttgtgccaa
     5881 taattaaatg aaatcacagc tatgcctgtt catggggttc taggccggaa ccccagattt
     5941 ttccgattgt ttgttttcta actgattatt cagtagtttt agagggctga cgctcagtgg
     6001 aacgaaaact cacgttaaga ggttttgact atgataaaag tcaaagtagg ggtgagtttg
     6061 gatatggaaa ttattgtact ataaggagag catgaaaacc cctggggcag tcactgaatg
     6121 agggaccagc agcggtaaaa taaccagcat aatttctgca tggtcgattt ttataccgtg
     6181 acaggctgtt ttttatactt ggggtcagtt tggatatcga aaattattgt acgttaaggt
     6241 tgttttttga ccttgtcgat cttaactgat ctcaaacgac gaaacttacc tgaagtcggt
     6301 atccggctgt ttttccagat ataatcgcct gtcagattga tatgttccca acctaatggt
     6361 gataaatgcg ataatagttg agcatttatt ttcatgccct tacgcttcag agcatcaacc
     6421 gctctttcca tgtaaactgt gttccataag gaaatagccg ccgtcaataa agtcaatcca
     6481 ctggcgcgat aactttgatt ttccggtttc cgatctctaa tctcccccaa tcgatgcatg
     6541 aatacagcac gggccagagc attacgggct tctcctttgt tcagcccggc ctggacacgt
     6601 cgccgcaatg ccggatcccg aaaccagtcc agcataaaca gtgttcgttc tatacggcca
     6661 atctctctca gagctttagc caacccattt tgtt
//
LOCUS       DAJTZL010000195         6660 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.196, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000195 DAJTZL010000000
VERSION     DAJTZL010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6660
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.196"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(1..767)
                     /locus_tag="OO544_003606"
                     /pseudo
     CDS             complement(1..767)
                     /locus_tag="OO544_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
     gene            1046..1999
                     /gene="tal"
                     /locus_tag="OO544_003607"
     CDS             1046..1999
                     /gene="tal"
                     /locus_tag="OO544_003607"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCR6769116.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            2114..2701
                     /gene="mog"
                     /locus_tag="OO544_003608"
     CDS             2114..2701
                     /gene="mog"
                     /locus_tag="OO544_003608"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HCR6769117.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAAGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(2736..3302)
                     /gene="satP"
                     /locus_tag="OO544_003609"
     CDS             complement(2736..3302)
                     /gene="satP"
                     /locus_tag="OO544_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HCR6769118.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(3451..4164)
                     /gene="msyB"
                     /locus_tag="OO544_003610"
     CDS             complement(3451..4164)
                     /gene="msyB"
                     /locus_tag="OO544_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HCR6769119.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKNMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVIQIACLRRMVSATQV"
     gene            complement(4190..4594)
                     /locus_tag="OO544_003611"
     CDS             complement(4190..4594)
                     /locus_tag="OO544_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HCR6769120.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            4971..>6660
                     /gene="dnaK"
                     /locus_tag="OO544_003612"
     CDS             4971..>6660
                     /gene="dnaK"
                     /locus_tag="OO544_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HCR6769121.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKT"
BASE COUNT         1784 a         1663 c         1691 g         1522 t
ORIGIN      
        1 taaggtatat cccgccgcgc cgcctgccgc ttcctgccag ccaaaagcac tctcaaaaat
       61 agaccaaatg acgtggggaa gttgcccgat atttattacg caaatcacca tgctggtcag
      121 cacccagatt atcgccatca acgggacaaa cccctgcatg agccgggcga cgccatgaag
      181 accgcgagtg attgccagca gagcaaagac agcgagaata atgcctgtca ccagcggggg
      241 aaaatcaaaa gaaaactcag ggctcgggca acggcgttcg cctgaactcc gctgaaaatt
      301 atgccgtagg cgatgagcaa aaagacggcg aacagaacgc ccatccaacg catccccagc
      361 ccgcgcgcca tataccatgc cggtcctcca cgaaactgtc cattgacgtc acgttcttta
      421 taaagttgtg caagggaaca ttcggcaaac gaggtcgcca tgccgataaa cgcggcaacc
      481 cacatccaaa agacggctcc aggtccaccg gcggtaatag ccagcgcaac gccggccagg
      541 ttgccgctac ccacgcgcgc cgcaagactg gtacacaatg actgaaatga ggttaaaccg
      601 cctggctgtg gatgaatgct atttttaaga cttttgccaa actggcggat gtagcgaaac
      661 tgcacaaatc cggtgcgaaa agtgaaccaa caacctgcgc cgaagagcag gtaaatcatt
      721 accgatcccc aaaggacgct gttaataaag gagaaaaaat ctggcatgca tatccctctt
      781 attgccggtc gcgatgactt tcctgtgtaa acgttaccaa ttgtttaaga agtatatacg
      841 ctacgaggta cttgataact tctgcgtagc atacatgagg ttttgtataa aaatggcggg
      901 cgatatcaac gcagtgtcag aaatccgaaa cagtctcgcc tggcgataac cgtcttgtcg
      961 gcggttgcgc tgacgttgcg tcgtgatatc atcagggcag accggttaca tccccctaac
     1021 aagctgttta aagagaaata ctatcatgac ggacaaattg acctcccttc gtcagtacac
     1081 caccgtagtg gccgacactg gggacatcgc ggcaatgaag ctgtatcaac cgcaggatgc
     1141 cacaaccaac ccttctctca ttcttaacgc agcgcagatt ccggaatacc gtaagttgat
     1201 tgatgatgct gtcgcctggg cgaaacagca gagcaacgat cgcgcgcagc agatcgtgga
     1261 cgcgaccgac aaactggcag taaatattgg tctggaaatc ctgaaactgg ttccgggccg
     1321 tatctcaact gaagttgatg cgcgtctttc ctatgacacc gaagcgtcaa ttgcgaaagc
     1381 aaaacgcctg atcaaactct acaacgatgc tggtattagc aacgatcgta ttctgatcaa
     1441 actggcttct acttggcagg gtatccgtgc tgcagaacag ctggaaaaag aaggcatcaa
     1501 ctgtaacctg accctgctgt tctccttcgc tcaggctcgt gcttgtgcgg aagcaggcgt
     1561 gttcctgatc tcgccatttg ttggccgtat tcttgactgg tacaaagcga atactgataa
     1621 gaaagagtac gctccggcag aagatccggg cgtggtttct gtatctgaaa tctaccagta
     1681 ctacaaagag cacggttatg aaaccgtggt tatgggcgca agcttccgta acatcggcga
     1741 aattctggaa ctggcaggct gcgaccgtct taccatcgca ccggcactgc tgaaagagct
     1801 ggctgagagc gaaggggcta tcgaacgtaa actgtcttac accggcgaag tgaaagcgcg
     1861 tccggcgcgt atcactgagt ccgagttcct gtggcagcac aaccaggatc caatggcagt
     1921 agataaactg gcggaaggta tccgtaagtt tgctattgac caggaaaaac tggaaaaaat
     1981 gatcggcgat ctgctgtaat cattcttagc gtgaccggga agtcggtcac gctacctctt
     2041 ctgaagcctg tctgtcactc ccttcgcagt gtatcattct gtttaacgag actgtttaaa
     2101 cggaaaaatc ttgatgaata ctttacgtat tggcttagtt tccatctctg atcgcgcatc
     2161 cagcggcgtt tatcaggata aaggcatccc tgcgctggaa gaatggctga catcggcgct
     2221 aaccacgccg tttgaactgg aaacccgctt aatccccgat gagcaggcga tcatcgagca
     2281 aacgttgtgt gagctagtgg atgaaatgag ttgccatctg gtgctcacca cgggcggaac
     2341 tggcccggcg cgtcgtgacg taacgcccga tgcgacgctg gcagtagcgg accgcgagat
     2401 gcctggcttt ggtgaacaga tgcgccagat aagcctgcat tttgtaccaa ctgcgatcct
     2461 ttcgcgtcag gttggtgtga ttcgcaaaca ggcgctgatc cttaacttac ccggtcagcc
     2521 gaagtctatt aaagagacgc tggaaggtgt gaaggacgct gcgggtaacg ttgtggtaca
     2581 cggtattttt gccagcgtac cgtactgcat tcagttgctg gaagggccat acgttgaaac
     2641 ggcaccagaa gtggttgctg cattcagacc gaagagtgca agacgcgacg ttagcgaata
     2701 aaaaaatccc cccgagcggg gggatctcaa aacaattagt gggattcacc aatcggcaga
     2761 acggtgcgac caaactgctc gttcagtact tcacccatcg ccagatagat tgcactggca
     2821 ccacagatca gaccaatcca gccggcaaag tggatgattg cggcgttacc ggcaatgtta
     2881 ccgatcgcca gcagggcaaa cagcacagtc aggctaaaga aaacgaattg cagaacgcgt
     2941 gcgcctttca gcgtgccgaa gaacataaac agcgtaaata cgccccacag acccaggtag
     3001 acaccaagga actgtgcatt tggcgcatcg gtcagaccca gtttcggcat cagcagaatc
     3061 gcaaccagcg tcaaccagaa agaaccgtaa gaggtgaatg cggttaaacc gaaagtgttg
     3121 ccttttttgt actccagcag accggcaaaa atttgcgcga tgccgccgta gaaaatgccc
     3181 atggcaagaa taataccgtc cagagcgaaa taacccacgt tgtgcaggtt aagcagaatg
     3241 gtggtcatgc cgaagcccat caggcccagc ggtgccggat tagccaactt agtgttgccc
     3301 ataattcctc aaaaatcatc atcgaatgaa tggtgaaata atttccctga ataacagtag
     3361 tgttttcagg gcgcggcata ataatcagcc agtggggcag tgtctacgat cttttgaggg
     3421 gaaaatgaaa attttccccg gtttacggtg tcagacctga gtggcgctaa ccatccggcg
     3481 caggcaggcg atttgtatta cggctggaat tgtcacgcga taggcactgc cgctgaccgc
     3541 tttaacccca tttaatgctg cgcccacagg gcctcccaga cccgcgccgc gcagcaaacc
     3601 atgcccaagc acgctcattg ctgcgtgggt gcgtaaaatg cgggtcaatt ggctggaaag
     3661 caaatgcgac acgccttttg ccaataattt gtctttcatc agcagcggca gcagctcttc
     3721 cagctcattc accctggcat cgaccgcgtg cagaaactcc tgcttatgtt cctcgtccat
     3781 attcttccag gtgttacgca gaaattgttc cagtaactgt tgctcaatct caaacgtaga
     3841 catctctttg tcggctttca gcttcaatcg ttttgaaaca tcgagcaaaa tggcccgata
     3901 caatttaccg tgtccgcgca gtttgttggc gatactatcg ccaccaaaat gctgtaattc
     3961 tccagcaatc agctgccagt tgcggcgatg ttgctcggga tgcccttcca tcgatttaaa
     4021 cagttcgttg cgcatcagta cgctggagag gcgagttttg cctttttcat tatgggtgag
     4081 caatcgggcg aaatttgcca actgttcctc actacaatgc tgaagaaaat ccagatctga
     4141 atcattcagg taattaacat tcattttttg tggtttctat attctggcgt tagtcgtcgc
     4201 cgataatttt cagcgtggcc atatccgatg agttcaccgt atgaccggaa aaggtgattt
     4261 ttgagacgca gcgtttattg tcgttatcac tgttaatgtt gatccagtca gtggtttgcc
     4321 cttcttttat ttctgaagga atattcagac tctgactggc gctacgggcg gctttgaaat
     4381 aaaccgatgc accgcttaac tgtaaatcgc catggtcggc agagagttgt atgcgtttca
     4441 caatgcgaca aacaggaagt ttcagcgcca gatcgttggt ttcgttacgc ggcattgcaa
     4501 tgacgccgag gagtttatgg tcgtttgcct gcgccgtgca gcacagcatc aggctaatcg
     4561 ccaggctggc ggaaatcgta aaaacggatt tcataaggat tctcttagtg ggaagaggta
     4621 gagggatgaa tacccactag tttactgctg ataaagagaa gattcaggta cgtaatcttt
     4681 tctttttatt acaatttttt gatgaatgcc ttggctgcga ttcattcttt atatgaataa
     4741 aattgctgtc aattttacgt cttgtcctgc catatcgcga aatttctgcg caaaagcaca
     4801 aaaaattttt gcatctcccc cttgatgacg tgggttacga ccccatttag tagtcaaccg
     4861 cagtgagtga gtctgcaaaa aaatgaaatt gggcagttga aaccagacgt ttcgccccta
     4921 ttacagactc acaaccacat gatgaccgaa tatatagtgg agacgtttag atgggtaaaa
     4981 taattggtat cgacctgggt actaccaact cttgtgtagc gattatggat ggcaccactc
     5041 ctcgcgtgct ggagaacgcc gaaggcgatc gcaccacgcc ttctattatt gcctataccc
     5101 aggatggtga aactctggtt ggtcagccgg ctaaacgtca ggcagtgacg aacccgcaaa
     5161 acaccctgtt tgcgattaaa cgcctgattg gccgccgctt ccaggacgaa gaagtacagc
     5221 gtgatgtttc catcatgccg ttcaaaatta ttgctgctga taacggcgac gcatgggtcg
     5281 aagttaaagg ccagaaaatg gcaccgccgc agatttctgc tgaagtgctg aaaaaaatga
     5341 agaaaaccgc tgaagattac ttgggtgaac cggtaactga agctgttatc accgtaccgg
     5401 catactttaa cgatgctcag cgtcaggcaa ccaaagacgc aggccgtatc gctggtctgg
     5461 aagtaaaacg tatcatcaac gaaccgaccg cagctgcgct ggcttacggt ctggacaaag
     5521 gtactggcaa ccgtactatc gcggtttatg acctgggtgg tggtactttc gatatttcta
     5581 ttatcgaaat cgacgaagtt gacggcgaaa aaaccttcga agttctggct accaacggtg
     5641 atacccacct ggggggtgaa gacttcgaca gccgtctgat caactacctg gttgaagaat
     5701 tcaagaaaga tcagggcatt gacctgcgca acgatccgct ggcaatgcag cgcctgaaag
     5761 aagcggcaga aaaagcgaaa atcgaactgt cttccgctca gcagaccgac gttaacctgc
     5821 cgtacatcac tgcagatgcg accggtccga aacacatgaa catcaaagtg actcgtgcga
     5881 aactggaaag cctggttgaa gatctggtaa accgttccat tgagccgctg aaagttgcac
     5941 tgcaggacgc tggcctgtcc gtatctgata tcgacgacgt tatcctcgtg ggtggtcaga
     6001 ctcgtatgcc aatggttcag aagaaagttg ctgagttctt tggtaaagag ccgcgtaaag
     6061 acgttaaccc ggacgaagct gtagcaatcg gtgctgctgt tcagggtggt gttctgactg
     6121 gtgacgtaaa agacgtactg ctgctggacg ttaccccgct gtctctgggt atcgaaacca
     6181 tgggcggtgt gatgacgacg ctgatcgcga aaaataccac tatcccgacc aagcacagcc
     6241 aggtgttctc taccgctgaa gacaaccagt ctgcggtaac catccatgtg ctgcagggtg
     6301 aacgtaaacg tgcagctgat aacaaatctc tgggtcagtt caacctggat ggtatcaacc
     6361 cggcaccgcg cggcatgccg cagatcgaag ttaccttcga tatcgatgct gacggtatct
     6421 tgcacgtttc cgcgaaagat aaaaacagcg gtaaagagca gaagatcacc atcaaggctt
     6481 cttctggtct gaacgaagat gaaatccaga aaatggtacg cgacgcagaa gctaacgccg
     6541 aagctgaccg taagtttgaa gagctggtac agactcgcaa ccagggcgac catctgctgc
     6601 acagcacccg taagcaggtt gaagaagcag gcgacaaact gccggctgac gacaaaactg
//
LOCUS       DAJTZL010000196         6648 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.197, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000196 DAJTZL010000000
VERSION     DAJTZL010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6648
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.197"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..738)
                     /locus_tag="OO544_003613"
     CDS             complement(<1..738)
                     /locus_tag="OO544_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HCR6769122.1"
                     /translation="MRHFIYQDEKSHKFWAVEQQGNELHISWGKVGTKGQSQIKSFSD
                     AAAAAKAELKLIAEKVKKGYVEQAKDNSLQPSQTVTGSLKVADLSTIIQEQPSFVAET
                     RAPDKNTDAVLPWLAKDIAVVFPPEVVHTTLSHRRFPGVPVQQADKLTQLRRLACSVS
                     QRDNKTATFDFSACSLEWQNTVAQAISQIDGLKTTQLPSPVMAVLTALEMKCTRYKVR
                     EDVMDQIVQEGGLEYATDVIIHLQQIDI"
     gene            complement(879..2912)
                     /gene="metG"
                     /locus_tag="OO544_003614"
     CDS             complement(879..2912)
                     /gene="metG"
                     /locus_tag="OO544_003614"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HCR6769123.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRSDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            3044..4153
                     /gene="apbC"
                     /locus_tag="OO544_003615"
     CDS             3044..4153
                     /gene="apbC"
                     /locus_tag="OO544_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HCR6769124.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLNALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            4398..4679
                     /locus_tag="OO544_003616"
     CDS             4398..4679
                     /locus_tag="OO544_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HCR6769125.1"
                     /translation="MNKYWLSGIIFLAYGLASPAFSSETATLAINGRISPPTCSMAMV
                     NGQPQPHCGQLTYNVDTRHLFSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
     gene            4922..5464
                     /locus_tag="OO544_003617"
     CDS             4922..5464
                     /locus_tag="OO544_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6769126.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGYYLYLGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLT
                     DNSVQSGDVLSSLVMRVAQD"
     gene            5544..6218
                     /locus_tag="OO544_003618"
     CDS             5544..6218
                     /locus_tag="OO544_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCR6769127.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLDIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNVKSNNANNWHLTIIDDHGN
                     YISDKI"
     gene            6234..>6648
                     /locus_tag="OO544_003619"
     CDS             6234..>6648
                     /locus_tag="OO544_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR6769128.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYTWDIGVFRLDFSVPQAWVEELE"
BASE COUNT         1757 a         1507 c         1593 g         1791 t
ORIGIN      
        1 aatatcaatc tgttgaaggt gtattattac atcagtagca tattccagac cgccttcctg
       61 gacgatctga tccataacat cctcacgcac tttatatctt gtgcatttca tttcaagtgc
      121 cgtgagtaca gccattactg gtgatggtaa ctgtgttgtt ttcaggccgt cgatctgact
      181 gatcgcctgg gcgacggtgt tttgccattc taaagaacag gcgctgaagt caaatgtggc
      241 tgttttatta tcccgttgcg acacactaca ggctaagcga cgtaattggg tcaatttgtc
      301 tgcttgctga acaggaactc cgggaaagcg gcgatgactt aacgtggtgt gtacaacttc
      361 gggcggaaaa acgacagcaa tatctttcgc cagccacggt aaaacagcat ctgtattttt
      421 gtcaggcgca cgggtttctg ctacgaaaga gggttgttcc tgaataatgg tggataagtc
      481 cgctaccttg agagagcccg ttaccgtttg ggaaggttgc aaagaattat ccttcgcttg
      541 ctccacatac cccttcttca ccttctccgc aatcagctta agctccgctt ttgccgctgc
      601 cgcagcatct gaaaaacttt ttatctggct ttgccctttg gtgccaactt ttccccaact
      661 gatatgcaac tcgtttccct gttgctcaac tgcccagaat ttatgtgatt tttcgtcctg
      721 atagataaag tgtctcattc tgcatccctg taattttatc cagttgaatg cagatgctac
      781 cagtatttat gcgggttaga gagagacaaa atcgcaagaa aacaacttta ggaatttata
      841 aagaaaaggc gctgtcgatg cagcgccttg aagagggatt atttcacctg atggcctggt
      901 ttagcaccag catccgggct taacaggaag atatctttcc cgccaggacc agcagccatt
      961 accatgcctt cggagatacc aaagcgcatt ttacgcggtg ccaggttggc caccataatg
     1021 gtgtgacgcc cgatcagtgc ctgcggatcc gggtaggcag aacgaatacc ggagaagaca
     1081 ttgcgttttt caccgccgag atccagtgtc aggcgcagca gtttgtcaga accttcaaca
     1141 aactctgcgt tttcgatcag cgccacgcgc aggtcaactt ttgcgaagtc gtcaaaggtg
     1201 atggtttcct gaatcggatc atctgccagt gggccagtaa ctggcgcggc agctgctttc
     1261 acttcttctt tagacgcttc caccagtgct tcaacctgct tcatatcgat gcggttatac
     1321 agcgctttga acggattcac tttgtggccc agcagcggtt gctggatacc atcccaggtc
     1381 agttctgtat tgaggaatgc ttctgcgcgc tcggtcagtt tcggcagtac cggcttcagg
     1441 taagtcatca gcacgcggaa caggtttatg cccattgagc aaattgcctg caggtcggca
     1501 tcgcggcctt cctgttttgc caccacccac ggagcctgct cgtcgacata gcggttagcc
     1561 agatcagcca gcgccatgat ttcgcgcact gctttaccaa attcgcggct ttcccacgct
     1621 tcaccaatca cttcagcggc atcagtgaag gttttgtaca actgcgggtc agccagttcg
     1681 cttgccagca cgccgtcaaa acgcttgttg ataaagcccg cattacgaga ggccaggtta
     1741 accactttgt tcacgatatc ggcattcaca cgctgaacga aatcctccag gttgagatcg
     1801 atatcatcaa tgcgcgaaga gagtttcgca gtgtagtagt aacgcaggct gtcagcgtca
     1861 aaatgattca gccaggtgct ggctttaata aaggtgccgc gagacttgga catctttgcg
     1921 ccgttcaccg tcacatagcc atgaacaaac aggttggtcg gcttgcggaa gttgctgcct
     1981 tccagcatgg caggccagaa caggctgtgg aagtaaacaa tatctttacc gatgaagtgg
     2041 tacagctcgg cggtggagtc tttcttccag tattcatcga agcttacgct gtcgctgcgc
     2101 ttgtcgcaca gattcttgaa agaacccatg tagccaatcg gtgcgtccag ccagacgtag
     2161 aaatatttgc ccggcgcgtt cggaatttca aaaccaaagt atggcgcgtc gcgggagata
     2221 tcccactgtt gcaggccgga ttcaaaccac tcctgcattt tatttgccac ctgctcctgc
     2281 aacgcaccgc tgcgggtcca tgcctgcaac atttcgctga aagagggcag atcaaagaag
     2341 aagtgttcag aatcacgcat taccggcgta gcgccagaaa ccaccgattt cggctcgatc
     2401 aattcagtcg ggctgtaggt cgcgccgcag acttcgcagt tatcgccgta ttgatccggg
     2461 gatttacatt tcgggcaggt gcctttcaca aaacggtccg gcaggaacat gcctttttcc
     2521 ggatcgtaca gctgagagat ggtgcggttt ttaataaaac cgttctcttt caggcgagag
     2581 tagataagtt ctgacaactg gcggttctct tcgctgtgcg tcgagtgata gttgtcatag
     2641 ctgatgttaa agcctgcgaa atcagtctga tgctcctgac tcatttcgcc aatcatctgc
     2701 tccggggtga taccaagctg ctgagctttc agcatgatcg gtgtaccgtg ggcatcgtcg
     2761 gcgcagatga agttgacctc gtggccgcgc attcgctggt aacggaccca gacatcagcc
     2821 tggatgtgct ccagcatatg gccgaggtgg attgagccgt tagcgtacgg cagtgcgcac
     2881 gtcaccagaa ttttcttcgc gacttgagtc atagtaggca ttacttctta atagtgaaaa
     2941 ggggttagat agtaccaaat gggaaaatgt taagtaagta cttctctata ttcttcaggt
     3001 aaactatcag ctgatggttt atctatacaa aaaaggagtc gggatgaacg aacaatccca
     3061 ggccaaatca ccagaagcct tgcgcgcaat ggtcgccggg acactggcca attttcagca
     3121 cccaaccctg aagcataacc tcaccacgct gaacgcgttg caccatgtcg cctggatgga
     3181 cgacacgttg catgtggaac tggttatgcc attcgtctgg catagcgcgt ttgaagagtt
     3241 aaaagagcaa tgtagcgccg aactgctgcg tatcaccggg gcgaaagcta tcgactggaa
     3301 actgtcgcac aatatcgcca ccttaaaacg cgtgaagaat cagccgggta ttaatggtgt
     3361 gaagaacatc attgcggtca gttccggtaa aggcggcgtg ggtaaatcct ccacggcggt
     3421 aaacctggcg ctggcactgg cggcggaagg ggcgaaagtc ggcattctgg acgccgatat
     3481 ctacggccca tcaattccca ccatgctggg cgcagaaaat cagcgtccaa cttcgccgga
     3541 cggtacccat atggcaccga ttatgtctca tggcctggca accaattcaa ttggttatct
     3601 ggtcactgac gacaatgcga tggtgtggcg tggaccgatg gcgagcaagg cgttaatgca
     3661 gatgttgcag gaaaccttgt ggccggatct cgactatctg gtgcttgata tgccgccagg
     3721 caccggtgac attcaattga cgctggcgca aaacattcca gtaactggtg cagtcgtggt
     3781 caccaccccg caggacatcg ctttgatcga tgcgaaaaaa ggcattgtga tgttcgagaa
     3841 agtcgaggtt ccggtgcttg gtattgtcga gaacatgagt gtgcatattt gcagtaactg
     3901 tggtcaccat gaaccgattt tcggtaccgg tggtgcagag aaactggctg aaaaatacca
     3961 cacacagctg ctggggcaga tgccgttaca catctcctta cgtgaggatc tcgataaagg
     4021 cacgccaacg gttatcagcc gtccggagag cgaatttacc gctatctacc gccagctggc
     4081 tgaccgcgtt gcagctcagc tctactggca gggtgaagtc attccaggcg agatttcctt
     4141 ccgcgcggtc taacgcctat taaccattct ggttattctt catacaccgc agagatgtta
     4201 cattgatgcg gtgtattagt ttttgccgca ttaatgaaca cttatttaat actgcgaata
     4261 ttctgcggac cttcgtccga aactctacaa agtcgattga tcatacaggc aatgcttcat
     4321 tatcatcctt cgtggctgtg tagtggatgt acagctatag tttgttatgc actcattcta
     4381 tccaggcata accttcaatg aataagtatt ggctgtcagg aattattttt cttgcctatg
     4441 gattggcttc tcccgcattc tcttcagaga ctgccacatt agcgattaat ggcaggatca
     4501 gtcccccaac atgcagtatg gctatggtta atggtcaacc tcaaccgcat tgtggtcagt
     4561 taacgtataa tgttgacacc cgtcatctgt tttcttcgcc cgtcaaaggt gtaacgacag
     4621 aagtggttgt tgcaggtagc gatagcaaac gtcgaatcgt gttaaatcgc tacgattaat
     4681 ttggcaacct gatttttatt gatatcaaca ataccattta cataaatcta cattaattga
     4741 tagatgcgat aaatagttaa tactcttaaa tatttgttgt atttatattg caatggttaa
     4801 tttgtttcaa ataaataaaa caatgtgttt acattgcggt aacttttttg atgtgagaaa
     4861 tttgtttcca agagatacag actcttaaca accaaaaaaa aattttatta aggaacatat
     4921 tatgaaacgt tcaattattg ctgccgctgt cttttcttct ttttttatga gcgctggagt
     4981 atttgctgca gacgttgata ccggaacatt aactattaag gggaatattg cagaatctcc
     5041 gtgtaaattc gaagcgggtg gtgattcagt aagtattaat atgccgactg taccaaccac
     5101 tgtctttgaa ggtaaagcta aatattctac ctatgatgat gcagtcggtg taaccagcag
     5161 catgttaaaa attagctgcc cgaaagaagt tgctggtgta aaactctcgt tgattaccaa
     5221 cgataaaata accggtaacg ataaggcgat agccagtagc aacgataccg tgggttacta
     5281 tctctattta ggtgataaca gcgatgtcct ggatgtttct gcacctttta acattgagag
     5341 ttataaaaca gcggaaggtc aatatgctat tccgtttaaa gcaaaatacc tgaaactgac
     5401 agataactca gtgcaatcag gtgatgtgtt atcttctctg gttatgcgtg tggcgcagga
     5461 ttaataaata aaattgtaag tttctccagg taataaacat ggccgctatc ccatggcggc
     5521 cttttaattt aagaggcatt aaaatgaaag gattattatc tttactcatt ttttctatgg
     5581 tccttcctgc acatgccgga attgttatct acgggacgcg cattatttac ccggcagaaa
     5641 ataaagaagt gatggtgcag ttgatgaacc agggaaaccg ttcttcgctg ctgcaggcgt
     5701 ggattgatga tggcgatacg tcattaccac cagaaaaaat tcaggttcct ttcatgttaa
     5761 cgccaccagt ggcaaaaata ggggcaaatt ccgggcagca agtaaaaatc aaaattatgc
     5821 cgaataaact gcccactaat aaagaaagca ttttttatct gaatgttctg gatattccac
     5881 caaatagtcc agagcaagaa ggtaagaatg cactgaagtt tgcgatgcaa aacagaatta
     5941 agttgtttta ccgaccagcg ggtattgctc cggtaaataa agcgacattt aaaaaattgc
     6001 tggtaaatcg cagtggcaat ggtttggtga taaaaaatga ctcagctaat tgggtgacga
     6061 tttcggatgt caaagctaat aatgtcaaag tcaattatga aactattatg attgccccct
     6121 tagaaagtca gagtgttaat gtcaaaagta ataatgcaaa taactggcat ctgaccatta
     6181 tcgatgacca tggcaactat attagtgaca aaatttaatt gcaggagctg cctatgttga
     6241 gaatgacccc acttgcatca gcaatcgtag cgttattgct cggcattgaa gcttatgcag
     6301 ctgaagaaac ctttgatacc cattttatga taggtggaat gaaagaccag caggttgcaa
     6361 atattcgtct tgatgataat caacccttac cggggcagta tgacatcgat atttatgtca
     6421 ataagcaatg gcgcgggaaa tatgagatta ttgttaaaga caacccgcaa gaaacatgtt
     6481 tatcaagaga agttatcaag cggttaggca ttaatagcga taacttcgcc agcggtaagc
     6541 aatgtttaac atttgagcaa cttgttcagg gtgggagcta tacctgggat atcggggttt
     6601 ttcgtctcga tttcagtgtc ccgcaggcct gggtggaaga actggaaa
//
LOCUS       DAJTZL010000197         6537 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.198, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000197 DAJTZL010000000
VERSION     DAJTZL010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6537
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.198"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            142..1317
                     /gene="mdtL"
                     /locus_tag="OO544_003620"
     CDS             142..1317
                     /gene="mdtL"
                     /locus_tag="OO544_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HCR6769129.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            1292..2251
                     /gene="yidZ"
                     /locus_tag="OO544_003621"
     CDS             1292..2251
                     /gene="yidZ"
                     /locus_tag="OO544_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HCR6769130.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA"
     gene            2408..3157
                     /locus_tag="OO544_003622"
     CDS             2408..3157
                     /locus_tag="OO544_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HCR6769131.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            3179..3729
                     /gene="yieF"
                     /locus_tag="OO544_003623"
                     /gene_synonym="chrR"
                     /pseudo
     CDS             3179..3729
                     /gene="yieF"
                     /locus_tag="OO544_003623"
                     /gene_synonym="chrR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
     gene            complement(3783..5120)
                     /gene="adeP"
                     /locus_tag="OO544_003624"
     CDS             complement(3783..5120)
                     /gene="adeP"
                     /locus_tag="OO544_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HCR6769132.1"
                     /translation="MSHQHTTQTSGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQIGMGAIFWGAVGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            5286..5951
                     /gene="yieH"
                     /locus_tag="OO544_003625"
     CDS             5286..5951
                     /gene="yieH"
                     /locus_tag="OO544_003625"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HCR6769133.1"
                     /translation="MSRIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     RFKGVKLYEIIDIVSLEHGVTLAKTESEHVYRAEVARLFDSELEAIEGAEALLSAITA
                     PMCVVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWD
                     ITA"
     gene            6018..6491
                     /locus_tag="OO544_003626"
     CDS             6018..6491
                     /locus_tag="OO544_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CbrB"
                     /protein_id="HCR6769134.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLLVIIQ
                     VLPLFLLLSITTGAIPALLTGVMVACLPEKIGSQKNYRCLAGGIGGVVITEIYCAVIV
                     HIKGMASSELFENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
BASE COUNT         1607 a         1650 c         1652 g         1628 t
ORIGIN      
        1 atttggctaa atccttcaag aagccagcca ttcgctggct tcttgcctct caggaaatca
       61 cttatgtcca aatggcaact cgcctgatcc tccttcacca cgtatgcttt gcgtcacctt
      121 actatcagga cgctttagcc catgtcccgc tttttgattt gtagttttgc cctggtttta
      181 ctttatcccg ccgggattga tatgtacctc gttggtttac cgcgcatcgc cgccgatctc
      241 aatgccagcg aagcgcagtt gcatattgcg ttctccgtat atctggcggg gatggcagct
      301 gcgatgttat ttgccggtaa agtggccgat cgttcaggga gaaagccggt cgccataccc
      361 ggcgcggcgc tatttattat tgcctcggtg ttctgttcac tggctgaaac cagcacgtta
      421 tttcttgcag gccgatttct acaggggttg ggcgcaggct gttgttacgt agtggcgttc
      481 gccattttgc gcgacacgct ggatgatcga cgtcgggcta aagtgctgtc attactcaac
      541 ggtattacct gcatcattcc ggtgttagcg ccagtgctcg gacatctgat tatgcttaaa
      601 ttcccgtggc agagtctgtt ctgggcgatg gcaatgatgg gcatcgcggt actgatgttg
      661 tctttgttta ttttaaaaga aacgcgccca gcgtcccccg ctgcttcgga caaaccacga
      721 gaaaatagcg agtcgctgct taatcggttt ttcctcagcc gtgttgttat caccaccctc
      781 agcgtttcgg tgatcctcac tttcgtcaat acatcgccgg tattgctgat ggaaatcatg
      841 ggttttgagc gcggagaata cgccaccatt atggcgttga ccgctggcgt cagcatgacc
      901 gtttcattct ccacgccatt tgcgctggga atttttaagc cacgtacgtt gatgatcacc
      961 tcgcaggtgt tattccttgc ggcggggatc acccttgccg tttcaccttc ccatgcggtt
     1021 tctctgtttg gtatcacgct gatttgcgcc ggtttctcgg taggttttgg cgtagcgatg
     1081 agtcaggcgt taggaccatt ttcattacgc gcgggcgtag ccagctcgac cttaggtatt
     1141 gcgcaggttt gcggttcgtc actgtggatt tggctggcag cggtggttgg tattggcgca
     1201 tggaatatgc tgatcgggat tctgattgcc tgtagcatag tgagcctgtt gctgattatg
     1261 ttcgtcgcgc ctggacgccc cgttgccgct catgaagaaa tccatcacca cgcttgatct
     1321 caatctgctg ctctgtctgc aactgctgat gcaggagcgc agcgtgacca aagcggcgaa
     1381 gcggatgaac gtgacccctt cagcggtgag taagtcgctg gcaaagttaa gagcgtggtt
     1441 tgacgacccg ctctttgtga actcaccgct gggcctgtcg cccacaccgc taatggtcag
     1501 catggagcaa aatctggcgg aatggatgca aatgagcaat ctgctgctgg ataaaccgca
     1561 ccaccagaca ccgcgcggcc tgaagtttga gttggcggcg gaatcgccgc tgatgatgat
     1621 catgcttaat gcgctgtcga aacggatcta ccaacgttac ccgcaggcga ccatcaaact
     1681 acgtaactgg gattacgatt ccttagatgc cattactcgt ggtgaagtgg atattggttt
     1741 ttccggtcgc gaaagccatc ctcgctcgcg ggagctgtta agctcgctac cgttagccat
     1801 tgattatgaa gtgctgttta gtgatgtacc ctgcgtctgg ttacgcaaag atcatccggc
     1861 actgcatgaa acgtggaatc tggacacctt tttacgttat ccgcatatca gcatttgctg
     1921 ggaacagagc gacacctggg cgctggacaa tgtgttacag gagctggggc gcgaacgcac
     1981 gattgctatg agtctgccgg aattcgagca gtcactgttt atggcagcgc aacccgacaa
     2041 tctgctactg gcgaccgcgc cgcgctactg tcagtactac aatcaactcc atcaactgcc
     2101 gttggttgct cttcctctcc cgtttgacga aagccagcaa aaaaagctgg aagttccttt
     2161 taccctgctg tggcataaac ggaacagcca taatccgaag atcgtctggt tacgggaaac
     2221 cattaaaaac ctttacgcgt cgatggcata accgaatcgt atgaaacgcg acccaatttc
     2281 acaataaaat gtaaaaaagt tgtaacaagc ttgtctgaat cgaactttta gccgctttag
     2341 tctgtccatc attccagtaa atgattactc ttgtattcat aatggaccat taagcatgga
     2401 gcgaaaaatg gcgactcact ttgcccgagg gattttaacg gaaggacatc tgatttctgt
     2461 tcgtctcccc tcccagtgtc atcaagaagc ccgaaacatt ccccctcatc gtcaaagccg
     2521 ttttctggcg tccagaggtt tactcgcaga actgatgttc atgctgtatg gcattggcga
     2581 attgccggaa atcgtcaccc tgccgaaagg taaaccggtt ttcagtgata aaaatttgcc
     2641 ttcgttttct atttcctatg ccgggaatat ggttggcgtg gcgttaacaa ccgaaggtga
     2701 atgtggcctc gatatggaac tacagcgtgc gacgcgcggg tttcatagcc cacacgcgcc
     2761 cgataaccac accttttcca gcaatgaatc gctatggatc agtaaacaaa acgatcctaa
     2821 cgaagcgcgg gcgcagctca tcacgctgcg ccgaagcgtg ctaaaactaa ccggtgatgt
     2881 tttgaatgac gatccgcgcg atctgcagct gctgcccatt gccggacgcc tgaaatgtgc
     2941 tcatgtaaat catgtagaag cgttatgcga cgcggaggac gtgctggtgt ggtccgtggc
     3001 ggtcacgccc gcgattgaaa agctcagtgt ctgggagtta gatggcaaac acagctggaa
     3061 aagcctgccg gatattcaca gccgcgccaa caatcctacc agccggatga tgcgttttgc
     3121 ccagctctct accgtgaagt ctttttcgcc aaattgatag acaaccacag gagtcattat
     3181 gtctgaaaaa ttgcaggtgg ttacgttact ggggagcctg cgcaaaggct catttaatgg
     3241 catggttgca cgtaccctgc cgaaaattgc tccggcgagc atggaagtca atgcgttacc
     3301 ttccattgcc gacattcccc tgtatgacgc tgacgtacag caggaagaag gttttccagc
     3361 aacggttgaa gctctggcgg aacagatccg tcaggctgac ggtgtggtga tcgtcacgcc
     3421 ggaatataac tactctgtac cgggtgggct gaaaaatgcc atcgactggc tttcccgctg
     3481 gccggtaaac cggtgttgat tcagaccagc tcaatgggcg tgattggcgg cgcgcgctgt
     3541 cagtatcacc tgcgccagat tctggttttc ctcgatgcaa tggtgatgaa caagccggaa
     3601 tttatgggcg gcgtgattca gaacaaagtt gatcggcaaa ccggagaagt gattgatcag
     3661 ggtacgctgg accacctgac cgggcaattg accgcatttg gtgagtttat tcagcgagtt
     3721 aagatctaaa taaaaaaccc gccagtaatc atgcatggcg ggtttttaat gcgctatcga
     3781 ttttaatgag cgtcgataaa tacaatcttc aggataaaca gcagcgcaac gatgattacg
     3841 cacgggctaa ggtcacgcag acgcccggta ccaatcttca tcacgcagta ggagataaag
     3901 cccagcgcaa taccttcggt aatcgaaaag ctgaacggca tcatcacggc ggtaataaac
     3961 gccggaacag attcagtaag atcctgccag ttcacgcgag caagacttga ggtcatcaac
     4021 acgccaacgt agatcaacgc gccagctgca gcgtagcctg gcaccattcc cgccagcggc
     4081 gacagaaaga taaccagcag gaacaacaga ccaacaacca ctgccgtcag accggtacga
     4141 ccgccaaccg atacgccgga agaggactca atataagccg taacggaaga agtaccgata
     4201 aacgaaccgg tcacggaaga gatactgtcg acatacagcg cctgcttcat gcgcgggaat
     4261 ttccccttct catccgccag acctgcttta tcggtcacgc caatcagcgt accggaggag
     4321 tcaaacaagt tgaccaacat gaaagagaaa atcacccctg ccagcccgag gttaaacgac
     4381 ccggctaaat ctacatgacc cacaactgtc attacgctcg gcggcgcaga aacgatgcca
     4441 ttgtagtgca catcacccag catccagccc agcagcgtcg tcaccacgat agaaaccagc
     4501 accgctgcgt gaatgttgcg cgaggccaga atggcgatga tgaagaaacc gaggatcccc
     4561 agcagcacgc tgtgagaagt cagattaccg atgctcacca gcgtttccgg gttagcgaca
     4621 atcacacctg cgtttttcag ccccatcatg ccaatgaaca gaccgatacc gctggtaata
     4681 cccacacgca gacttaccgg aatgttagca atcatccagt aacgaacgcg gaaaatggtc
     4741 aaaagaagca gtccgacagc gccccagaag attgcgccca taccgatctg ccacggcagc
     4801 cccattgcct ggacaaccac aaaagcgaaa aaggcgttaa gccccatcgc aggagccagc
     4861 gccaccggaa ggtttgcaaa caatcccatc ataatgctac cgaaagcagc aatcagacag
     4921 gttgttacga agacggcact ggtgtccatg ccagcaacgc ccagaatttg tgggttaaca
     4981 aatacgatgt agaccatcgt caggaaggtg gtgaacccgg caatcacttc agttcgtgcc
     5041 gtagtaccat gttcgcgcag tttaaaaact cgttccagca tcccctgacc agaagtctgg
     5101 gtggtatgtt gatgactcat tatctatttc cgaacaagga gggaaaatcc gtcgctatcg
     5161 tataccaaaa tgcgacaata ggcgcgtttg tgagagactt tttttattgg atttacttat
     5221 acggcaacga ttgcgttgcg caaatcggct ctacgaaaac gttaaactga ttaaaaagga
     5281 aaggcatgtc ccggatagaa gcggtatttt tcgactgcga cggtacgctg gtcgacagtg
     5341 aagtcatttg ctcccgcgca tatgtaacga tgtttcagga atttggtatt acgctcgatc
     5401 ctgaagaggt attcaaacgt ttcaaaggtg taaaactgta cgaaattatc gatattgttt
     5461 cccttgaaca tggtgttacg ttagcgaaaa cagaatctga acacgtttac cgtgcagaag
     5521 tcgctcggct gttcgattca gaactggaag ccatcgaagg ggctgaagcg ctcctgtcag
     5581 cgatcactgc gccaatgtgt gtggtatcta acggcccaaa taacaaaatg cagcattcta
     5641 tgggcaagct gaatatgttg cactacttcc cggataaact gttcagcggc tacgatattc
     5701 agcgctggaa gccagacccg gcgttaatgt tccatgcggc aaaagcgatg aatgtaaatg
     5761 tagaaaactg cattctggtt gatgactcag ttgccggtgc gcaatctggt atcgacgcag
     5821 gtatggaagt gttctacttc tgcgccgacc cgcacaataa gccgatcgtt cacccgaaag
     5881 tcaccacctt tacccatctt tcgcagttac cggaactgtg gaaagcgcgt ggttgggata
     5941 ttacggcata gttcttcaca ctcccttcac ttaccccgct taaattggcg ctcaaaggta
     6001 agtaaaggga gtttgatatg tctgtttcac gtcgggtaat acatcacgga ctttattttg
     6061 cggttttagg accgttaatt ggtgttctgt ttcttgtcct ctacatattc ttcgcaaaag
     6121 aaccgctggt tcttttggta ataatacagg tacttccatt gtttctctta ttgtcgatta
     6181 ctacgggagc tattcctgca ttgttaaccg gcgtgatggt cgcctgtctg ccggaaaaga
     6241 tcgggtcaca gaaaaattat cgttgtctgg ctggtggcat aggtggcgtc gttatcaccg
     6301 agatctattg tgcagttatt gtacatatta agggcatggc ttcctcggag ttgtttgaaa
     6361 acattctttc tggtgacagt ctcgttgtcc gcatcattcc tgcattgctg gcaggtgtgg
     6421 tgatgagcag aatcattacc cgtctgcccg gattggatat ttcatgtcct gaaacagact
     6481 ctttaagtta agcgggatac tttatctttg ggctactcaa aagcagacag gatgtta
//
LOCUS       DAJTZL010000198         6535 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.199, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000198 DAJTZL010000000
VERSION     DAJTZL010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6535
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.199"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..548)
                     /locus_tag="OO544_003627"
     CDS             complement(<1..548)
                     /locus_tag="OO544_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCR6769135.1"
                     /translation="MAVKISGVLKDGTGKPVQNCTIQLKAKRNSTTVVVNTLASENPD
                     EAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSRPGTLNDFLGAMTEDDARPEA
                     LRRFELMVEEVARNASAVAQNTAAAKKSASDASTSAREAATHATDAADSARAASTSAG
                     QAASSAQSASSSAGTASTKATEA"
     gene            complement(613..1129)
                     /locus_tag="OO544_003628"
                     /pseudo
     CDS             complement(613..1129)
                     /locus_tag="OO544_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
     gene            complement(1200..4697)
                     /locus_tag="OO544_003629"
     CDS             complement(1200..4697)
                     /locus_tag="OO544_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HCR6769136.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVEGPVDGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRAGEQEQSPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGSTEPRITCNAWLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRQSDKVWAYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPDNGWETATELVEDTQAIVRYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGIRTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGQGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     HQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVTADDGSERLVSTARTTETTYRFTQ
                     LALGNYRLTVRAVNAWGQQGDPASVSFRIAAPAALSRIELTPGYFQITATPHLAVYDP
                     TVQFEFWFSEKRIADIRQVETTARYLGTALYWIAASINIKPGHDYYFYIRSVNTVGKS
                     AFVEAVGQPSDDASGYLDFFKGEIGKTHLAQELWTQIDNGQLAPDLAEIRTSITDVSN
                     EITQTVNKKLEDQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMQDGRLYIAGIGAGIEN
                     TPDGMQSQVLLAADRIAMVNPANGNTKPMFVGQGDQIFMNDVFLKRLTAPTITSGGNP
                     SAFSLTPDGKLTAKNADISGSVNANAGTLNNVTVNENCTIKGMLEATQVRGDFVKAVS
                     KSFPKQAGTWGNTETPNGTVTVTISDDHNFDRQIIIPPIIFNGIAYDDPGSGNNPGGT
                     RYTGYGFEVRKNGVLIASRETKGAIPGSYSAVIDMPSGGGSVTLEFKIFQKGNQGAGN
                     ITDCTVIVTKKAASGISIR"
     gene            complement(4758..5390)
                     /locus_tag="OO544_003630"
     CDS             complement(4758..5390)
                     /locus_tag="OO544_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HCR6769137.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAIRAMATQLPAFRQKLSD
                     GWYQVRIAGQDVSTSGLTAQLHEALPDGAVIHIVPRVAGAKSGGVFQIVLGAAAIAGS
                     FFTAGATLAAWGAAIGAGGMTGILFSLGASMVLGGVAQMLAPKARTPRTQTTDNGKQN
                     TYFSSLDNMVAQGNVMPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            complement(5327..6070)
                     /locus_tag="OO544_003631"
     CDS             complement(5327..6070)
                     /locus_tag="OO544_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6769138.1"
                     /translation="MTETESAILAHARRCAPAESCGFVVRTPEGERYFPCVNISGEPE
                     AYFRMSPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHREDDWWRHGQNLYLDNLEAT
                     GLYQVPLSAAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWHASAFTGICNDLAAASTFV"
     gene            complement(6076..>6535)
                     /locus_tag="OO544_003632"
     CDS             complement(6076..>6535)
                     /locus_tag="OO544_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HCR6769139.1"
                     /translation="VSNLYGMVTGMAEDLQSLVGGTVVRRKVYARFLDAVNFVNGNSD
                     ADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDGAVFPGRIMLANTCTWTYRGDEC
                     GYHGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNVGNFGGFLSINKLSQ"
BASE COUNT         1372 a         2039 c         1636 g         1488 t
ORIGIN      
        1 gcttcagtgg cctttgttga tgccgttcct gcgctggaag acgctgactg agccgacgaa
       61 gcggcctgtc cggctgacgt gctggctgca cgtgctgaat ctgcagcatc agtcgcatgg
      121 gttgccgcct cacgggctga tgtgctggca tcgctggctg atttcttcgc ggctgccgtg
      181 ttctgtgcca ccgcggacgc gttacgcgcc acctcttcca ccatcagctc aaaacggcgc
      241 agtgcctccg gacgggcatc atcctccgtc atggcaccga gaaaatcatt cagcgtaccg
      301 ggtcgggaat cttcatacac ggtgatggtc ccggcatgtg acggcgggaa tccttccacc
      361 aacagaataa cgctgtactg accgtactca acgtccatgc tgtaacgccc ggcttcatcc
      421 ggattttctg aggccagcgt gttcaccacc accgtggtgc tgttacgttt tgctttcagc
      481 tggattgtgc agttctgtac cggttttcct gtgccgtctt tcagtacacc tgaaatcttt
      541 actgccatat tcaccccaca aaaaagcccg cctgaaccgg cgggctgtca taacactgtg
      601 ttacctggct aatcagaatt tataaccgac acccacgatg aaaccgtcag tgcgccagag
      661 acgtgttgct gtggcgaccg tcatcacttc cggtcagcac atcgtgcgtt ttccccttat
      721 tgtcagttac gcggatataa tccccggaaa aagtcgacac acggctgtaa gccacacccg
      781 ccatcgcata cgcgctgaac cattcattca cgcgcacaga cggccccgcc atcacgctga
      841 accagcggtt acgcacggaa tcttcatgcc agcgggtatc gctgtaacgg gtcagctggc
      901 gattcttgtc tcctgcatag ctgaatgacg tcaccagccc cagcgtatcc gtaaactcat
      961 aacggtattt cacgttaatc ccgttcagat catcactgcc gggaacgttg gtccgggcat
     1021 gaagataccc cgcgctcagc gtggactgat gttcagacgc ccatgcaggc gcaccggata
     1081 cggccaggca aatggctgcg gacaaaatgg ctgcacaaac tttacgcata attacctctc
     1141 gcttttctgc aataaaaaag gcgtcattcc tgacgccatt tattggggtt ataaatattt
     1201 caacgaatac tgatgccgga agccgctttt ttggtcacaa tcaccgtaca gtcggtgata
     1261 ttgcctgccc cctgattgcc tttctggaaa atcttaaact ccagagtgac gctaccacca
     1321 ccactaggca tatcaataac tgcactgtaa ctaccgggaa tggcaccttt agtttctctg
     1381 gatgcgatta atacgccgtt tttgcgaact tcaaaaccat aacccgtgta tcgcgtgcct
     1441 cctgggttat ttccgctccc cggatcgtca tacgctatac cgttaaaaat aatgggcgga
     1501 ataataatct ggcggtcaaa gttatgatca tcgcttatgg tgactgtaac cgtcccgttt
     1561 ggtgtttccg tgttacccca cgtaccagcc tgtttcggaa atgatttgga tacagcttta
     1621 acgaagtcac ctctgacctg agtcgcctcc agcatgccct taatcgtaca gttttcattt
     1681 accgtgacat tgttgagcgt cccggcgttc gcattcacac tgccactgat atccgcattt
     1741 ttagcggtca gctttccgtc cggagtcagg gaaaatgccg atggatttcc accgctggta
     1801 atggtggggg ccgtcaggcg tttcaggaac acgtcgttca tgaatatctg atcgccctga
     1861 ccaacaaaca tcggttttgt gttgccattc gcaggattaa ccatcgcaat cctgtccgcc
     1921 gccagcagca cctgactctg catgccgtca ggggtgttct caataccggc accaataccc
     1981 gcgatataaa ggcgtccgtc ctgcatctgc tgcagcttca cagcccacat gctgttcagg
     2041 ttattatttg tatcaacctg aaccttctgt atctgctgaa ttgccgcact ctggtcttcc
     2101 agtttcttat tgacggtctg cgtgatttca ttgctgacat ccgtaatgga cgtcctgatt
     2161 tcagccaggt caggcgcaag ctgaccgtta tcaatctgcg tccacagctc ctgagccaga
     2221 tgggttttcc ctatctcgcc tttgaaaaaa tccagataac cggatgcatc atcactcggc
     2281 tgaccaacag cctccacgaa tgccgatttg ccaacggtgt tcacactgcg gatataaaag
     2341 taataatcat ggcccggttt gatattgata ctggcggcta tccagtacag cgccgtgcca
     2401 agatagcgtg ctgtggtttc aacctgcctg atatccgcaa tccgcttttc cgagaaccag
     2461 aactcaaact gtaccgtcgg atcataaacc gcaagatgcg gcgtggcggt tatctgaaaa
     2521 tagcccggcg tcagctcaat ccgcgacagc gctgccggtg cggcaatccg gaacgatacc
     2581 gacgccggat cgccctgctg cccccacgca tttactgccc ggactgtcag cctgtagttc
     2641 cccagcgcca gttgtgtgaa gcggtaagtg gtttccgtcg tccgggccgt gctgaccagc
     2701 cgctcactgc cgtcatccgc tgttacggtc agacggagca ggaagctcac gcccttcacc
     2761 accttcggcg tgtcccagcg cgccagcacc tgatattccc cgctgtctgc ggtgacttcg
     2821 gcagtcaggt gctgcaccgc tggcggcgtg acaccattca ccgtgccgct ctggtcgccg
     2881 tcaaagtgcg ccccgttatc cacgatggct tctttttccg gcacatgctg cacggcggtg
     2941 atggcatacg tgccgtcgtc gttctcacgg atactcacgc agcggaacaa tcgctggtgc
     3001 agcgtcggca gcttcagccc ccacacgctg tattcagcaa caccgtcagg aacacggctc
     3061 acttttacct tcacgccgtc ggtgacggac tggacctcca cgctgaccgg attcccctgc
     3121 ccgtcaacca ggcttatcag cgtggtgccg gaggatggca gcgtgatttc acggtcgagc
     3181 gtcagcgtcc gggtctggct gttcaccgcc agcacgcgcc cgccggtgcg gataccggca
     3241 tagtcatcat cgcagatttc aatgacatcg cccggtacat gacgaagccc ttcagcaccc
     3301 acgctgaaat ccacggtctg cgtctccagc agttccgttt taatcagcca cagcccggcg
     3361 cggtgtgcct gcccccggct ggtacagcca aaggcatcca tcttcgtgac gttacgaccg
     3421 taacggacaa tggcctgcgt atcctccaca agctctgtcg ccgtctccca gccgttatcc
     3481 gggtcaatcc agttcacctc aacggcatta tggcggtctt tcagggcgct gaaactgtag
     3541 cggaacggcg cgccatcatc cggcatcacc acattactgc ggttataggc ccacacctta
     3601 tccgactgtc ggtcctgcac gaacgtcagc gtctgcccgt tccataccgg catacagcgc
     3661 atcgccgagc agaaatcact gagcacatcc cacgccttgc gctgtgtggt cagccaggca
     3721 ttacaggtga tgcgcggctc cgtgctgcca aagccgtccg gcaccgactg gtcacagtac
     3781 tggccgatga catacagcgc ccatttatcc acatctgccg caccaagacg tttccccatg
     3841 ccatagcgcg gatgggtcag catatcccac agacaccagg ccatgttgtt gctgtatgcc
     3901 ggttttaacg ttccgtccca gataccgctg tattgccgcg tctgcggatt atagttcgac
     3961 ggcacctgca gaatgcgccc gcgaagatga taattacggc tcacctgctg gctgccgaac
     4021 tgctccgaat ccacctgtac gccgaccagt gccgtgttcg ggtagcactg tttcacatcg
     4081 atgatttcgg tgtatgacga ccagagcgtt ttgttctgca gctggtctgt ggtgctgtcc
     4141 ggcgtcattc tgcgcatccg gatattaaac gggcgcggcg gcaggttatc caccaccacc
     4201 gaggccagat actgcgaggt ggttttgccc ttaatggtga tgtctttttc cgtcacccag
     4261 ccaccgttac gctgtatctg aaccagcagg cgaacttcag acggattcct gtctcccttt
     4321 gaggtggttt ccaccagtgc ctgtacaccg aaggtaaaac gcagtcggtc aatgtttgcc
     4381 gacgtgatgg tccgggtgat cggcgtgtca tatttcactt ccgtacccag caccgtctcg
     4441 gagccggagg attcaaatcc ctccggcggt gactgctcct gctcacctgc ccggaacacc
     4501 accgtgacgc cggagatgtt ggtattcccc tcactgtcca gcaccggcgt actgttcagc
     4561 agcacgcttt ttaatccatc caccggacct tcaaccggcc cttcgctgat ggcatcgatc
     4621 acactcagca gctgcgtgga cttcaggttg tccttcgctt cgcgcggggt atgcccctta
     4681 ctgctgcctt tacccattcg tcatgctcca taaacgataa aaccgcccgg aggcgatttc
     4741 acataaaaca ttttgcatca gcgaccaatc accacaacct gaccaccgtc cccttcgtct
     4801 gccgtgctga tctcctgaga taccacacgt gaccccacgc gcatttcacc atacagaaca
     4861 ggcataacat tgccctgggc aaccatgtta tccagtgagg agaaataggt gttctgctta
     4921 ccgttatccg ttgtctgtgt gcggggagtt ctggctttcg gtgccagcat ctgagccaca
     4981 ccgccgagca ccatactggc accgagagaa aacagaatgc cggtcatacc accggcacca
     5041 atggcagccc cccatgctgc aagggtggct ccggcggtaa agaatgatcc ggcaatggcg
     5101 gctgctccca ggacaatctg gaatacacca cctgacttgg ccccggcgac tctgggaaca
     5161 atatgaatca cagcgccgtc aggcagagcc tcatgtaact gtgccgttaa tccggacgtg
     5221 ctaacatcct gcccggcaat acgcacctga taccagccgt cgctcagctt ctgacgaaac
     5281 gcagggagct gtgtggccat cgcccggatg gcttcggcac ccgttttcac acgaaggtcg
     5341 atgcggcggc caaatcgttg caaatccccg taaaggcaga tgcgtgccat gcccggtgac
     5401 gccagaggga gtgtgtgcgt cgctgccatt tgtcggtata cctctctcgt ttgctcagtt
     5461 gttcaggaat atggtgcagc agctcgccgt caccacagta aatggcggca tgattcggca
     5521 ccgatgaacc aaaacagcac agcagcacat cgcccggctg cgccgctgac aacggcacct
     5581 gatacagccc tgtggcctcc agattatcca gatagagatt ctgaccgtga cgccaccagt
     5641 catcctcacg atgaaaatcc ggcatctcaa tccccgccag atggtaagca tcccggaaca
     5701 gcgtgtaaca gtccgtcacc ccgtgctcaa agcgccgccc ggtaagatgt ggcacacagc
     5761 ggaacttgtg aatcgccccc cggcagacca gccaccacgg caaatcactc tgcacctgca
     5821 gccgccggtc ggcctcactc agccagggca gaccaccggg gtggctgtgg accagtgcca
     5881 caatctcacc ctgcatctct gcctgcagcc agtcttccgg cgacatacgg aaatacgcct
     5941 ccggctcacc ggagatattc acgcagggga aatatctttc cccctccggc gttcttacca
     6001 cgaagccgca cgactccgct ggagcacatc gccgggcgtg cgccagaatc gctgattctg
     6061 tctctgtcat gggatttact gcgaaagttt gttaatggaa aggaagccgc caaagttgcc
     6121 gacgttattg cgaaacttac agccactcag gcatttgctg catttatcct tcgtgatgtc
     6181 ggacgttggc tggtcatatt catccgcgac cgccggaccg tgataaccgc actcatcgcc
     6241 gcgataggtc caggtgcagg tgttggccag catgatacgt cccggaaaaa cagcgccatc
     6301 tgtttccgtc ggcgtggaca gtacaaaaga ggcactcacc gcgctcagtt cgctgcactg
     6361 ctcaatgcgc cagcggctga tcacctcctg ctccggatcg gcgtcactgt ttccgttgac
     6421 gaagttcacc gcatccagaa aacgggcgta aaccttacgc cggaccaccg ttccgccgac
     6481 cagactctgt agatcttccg ccatcccggt gaccataccg tacaggttag aaacc
//
LOCUS       DAJTZL010000199         6530 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.200, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000199 DAJTZL010000000
VERSION     DAJTZL010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6530
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.200"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..906)
                     /locus_tag="OO544_003633"
     CDS             complement(<1..906)
                     /locus_tag="OO544_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter YfaL"
                     /protein_id="HCR6769140.1"
                     /translation="MRIIFLRKEYLSLLPSMIASLFSANGVAAAIDLCQGYDIKASCH
                     ASRQSLSGITQDWSIADGQWLVFSDMTNNASGGAVFLQQGAEFTLSPENETGMTLFAN
                     NTVTGEYYNGGAIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGAVDLSVTNA
                     MFRNNIANDGKGGAIYTINNDVYLSDVIFDNNQAYTSTSYSDGDGGAIDVTDNNSDSK
                     HPPGYTIINNTAFTNNTAEGYGGAIYTNSATAPYLIDISVDDSYSQNGGVLVDENNSA
                     AGYGDGPSTAAGGFMYLGLSEVTFDI"
     gene            1602..3887
                     /gene="nrdA"
                     /locus_tag="OO544_003634"
     CDS             1602..3887
                     /gene="nrdA"
                     /locus_tag="OO544_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HCR6769141.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINNLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDTIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            4121..5251
                     /gene="nrdB"
                     /locus_tag="OO544_003635"
     CDS             4121..5251
                     /gene="nrdB"
                     /locus_tag="OO544_003635"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HCR6769142.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            5251..5505
                     /gene="yfaE"
                     /locus_tag="OO544_003636"
     CDS             5251..5505
                     /gene="yfaE"
                     /locus_tag="OO544_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HCR6769143.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEM"
     gene            complement(5582..6208)
                     /gene="inaA"
                     /locus_tag="OO544_003637"
     CDS             complement(5582..6208)
                     /gene="inaA"
                     /locus_tag="OO544_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005136699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HCR6769144.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYARHAVSPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRNKAINHDFRQLENIWSQSPKRTGSR"
     gene            6387..6530
                     /locus_tag="OO544_003638"
                     /pseudo
     CDS             6387..6530
                     /locus_tag="OO544_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
BASE COUNT         1685 a         1685 c         1526 g         1634 t
ORIGIN      
        1 aatatcaaag gtaacttcac ttaagccgag atacataaag ccacccgccg cagtggaagg
       61 accatctcca tagcctgctg cgctgttgtt ctcatccact aacacgccgc cgttctggct
      121 gtagctgtca tcaacagaaa tatcaataag atagggagcc gtcgcgctat tggtatatat
      181 cgccccgcca taaccttcgg cagtgttatt tgtaaaggca gtgttattta ttatcgtata
      241 acctggagga tgcttgctgt cgctattatt atcggtaaca tcgattgccc cgccatcgcc
      301 atcactgtaa cttgttgatg tatatgcctg gttgttatca aaaataacat cacttaaata
      361 gacgtcatta ttaatggtat aaattgcgcc acctttgcca tcattagcga tattattgcg
      421 aaacatggcg ttagtgacac ttaaatcgac ggcaccagta tcgttagtac cagaagaata
      481 gattgcgcca ccatagccgc ctgcgacgtt accggaaaaa atgacatcag taagattcag
      541 cgttgagttt tctttagcaa atatggcccc gccgttataa tattctcctg taacggtgtt
      601 attggcaaac agagtcattc cagtttcgtt ttctggtgat aatgtaaatt ccgctccttg
      661 ttgcaaaaat acggccccac cgctggcatt attggtcata tccgaaaaaa ccagccattg
      721 cccatcggcg atactccagt cctgcgtaat gcctgaaagg ctttgcctgc tggcgtgaca
      781 actcgctttg atatcatatc cctggcataa atcaatggcc gccgcgacac cgttagcaga
      841 gaaaagagat gcaatcattg acgggagtag agataaatac tccttgcgta gaaagataat
      901 ccgcatatta atctgaccat cattttatat aagacggcat atttaaacgc attatacaat
      961 atgggttatc tttcttttgt aaattgtcca acccgatgca atggctattt tgttattatt
     1021 tgatgaataa tatcagtgcg tcataattca agttaataac cttaagggat accagttata
     1081 tttaaactaa attatagtaa tgaataattt tcttataata taaggtaaat taacaaaatg
     1141 gcttcgcatt taacaataac cgaatagaaa acaaccattt cgacatcaac aatcgcttac
     1201 attcgcttat atattgacca caactgatac atcagattat gtgatgactc gtgcttagat
     1261 caatttttgc aatcattaac aaaaagatta ataaaccatc tatatcaatt tatccaacct
     1321 attctgccgt tcaagaaatc gccgaacagt tatttttaac aaatttttct cttcccattg
     1381 actttcccgg acaccttgtc tgacctaagg tgcgcgaaag ccactttttc cttcctgagt
     1441 tatccacaaa gttatgcact tgcaagaggg tcattttcac actatcttgc agtgaatccc
     1501 aaacataccc cctatatata gtgttctaag cagcttccct tactacaggt agtctgcatg
     1561 aaactattgc ggaaagaatt ccaaaaacag gtacgacata catgaatcag aatctgctgg
     1621 tgacaaagcg cgacggtagc acagagcgca tcaatctcga caaaatccat cgcgttctgg
     1681 attgggcggc agaaggactg cataacgttt cgatttccca ggtcgagctg cgctcccaca
     1741 ttcagtttta tgacggtatc aagacctctg acatccacga aaccattatc aaggctgccg
     1801 cagacctgat ctcccgtgat gcgccggatt atcagtatct cgccgcgcgc ctggcgatct
     1861 tccacctgcg taaaaaagcc tacggccagt ttgagccgcc tgcgctgtac gaccacgtgg
     1921 tgaaaatggt cgagatgggc aaatacgata atcatctgct ggaagactac acggaagaag
     1981 agttcaagca gatggacacg tttatcgatc acgaccgtga tatgaccttc tcttatgctg
     2041 ccgttaagca gctggaaggc aaatatctgg tacagaaccg cgtgaccggc gaaatctatg
     2101 agagcgccca gttcctttat attctggttg ccgcgtgcct gttctcgaac tacccgcgtg
     2161 aaacgcgcct gcaatatgtg aagcgttttt acgacgcggt ttccacattt aaaatttcgc
     2221 tgccgacgcc aatcatgtcc ggcgtgcgta ccccgactcg tcagttcagc tcctgcgtac
     2281 tgatcgagtg cggtgacagc ctggattcca tcaacgccac ctccagcgcg attgttaaat
     2341 acgtttccca gcgtgccggg atcggcatca acgccgggcg tattcgtgcg ctgggtagcc
     2401 cgattcgcgg tggtgaagcg ttccataccg gctgcattcc gttctacaaa catttccaga
     2461 cagcggtgaa atcctgctct cagggcggtg tgcgcggcgg tgcggcaacg ctgttctacc
     2521 cgatgtggca tctggaagtg gaaagcctgc tggtgttgaa aaacaaccgt ggtgtggaag
     2581 gcaaccgcgt gcgtcatatg gactacgggg tacaaatcaa caaactgatg tatacccgtc
     2641 tgctgaaagg tgaagatatc accctgttca gcccgtccga cgtaccgggg ctgtacgacg
     2701 cgttcttcgc cgatcaggaa gagtttgaac gtctgtatac caaatatgag aaagacgaca
     2761 gcatccgcaa gcagcgtgtg aaagccgttg agctgttctc gctgatgatg caggaacgtg
     2821 cgtctaccgg tcgtatctat attcagaacg ttgaccactg caatacccat agcccgtttg
     2881 atccggccat cgcgccagtg cgtcagtcta acctgtgcct ggagatagcc ctgccgacca
     2941 aaccgctgaa cgacgtcaac gacgagaacg gtgaaatcgc gctgtgtacg ctgtctgctt
     3001 tcaacctggg cgcaattaat aacctggatg aactggaaga gctggcaatt ctggcggttc
     3061 gtgcacttga cgcgctgctg gattatcagg attacccgat cccggccgcc aaacgtggag
     3121 cgatgggtcg tcgtacgctg ggtattggtg tgatcaactt cgcttactac ctggcgaagc
     3181 acggtaaacg ctactccgac ggcagcgcca acaacctgac gcataaaacc ttcgaagcca
     3241 ttcagtatta cctgctgaaa gcctctaatg agctggcgaa agagcaaggc gcgtgcccgt
     3301 ggtttaacga aaccacttac gcgaaaggga tcctgccgat cgatacctat aagaaagatc
     3361 tggataccat cgctaatgag ccgctgcatt acgactggga agcattgcgt gaatcaatca
     3421 aaacgcacgg cctgcgtaac tctacgcttt ctgccctgat gccgtccgag acctcttcgc
     3481 agatctctaa cgccactaac ggtattgaac cgccgcgcgg ttacgtcagc atcaaagcgt
     3541 cgaaagacgg tattttgcgc caggtggtgc cggactacga gcacctgcac gacgcctatg
     3601 agctgctgtg ggaaatgccg ggtaacgatg gctacctgca actggtaggt atcatgcaga
     3661 aatttatcga tcagtcgatc tctgctaaca ccaactacga tccgtcacgt ttcccgtcag
     3721 gaaaagtgcc gatgcagcag ttgctgaaag acctgctcac cgcctacaaa ttcggggtca
     3781 aaacgctgta ttatcagaac acccgtgacg gcgctgaaga cgcgcaggac gatctggtac
     3841 cgtcaatcca ggacgatggc tgcgaaagcg gcgcatgtaa gatctgatat tgagatgccg
     3901 gatgcggcgt aaacgcctta tccggcctac ggctcggttt gtaggcctga taagacgcgc
     3961 cagcgtcgca tcaggctccg ggtgccggat gcagcgtgaa cgccttatcc ggcctacggc
     4021 tcggatttgt aggcctgata agacgcgcca gcgtcgcatc aggcacagga tgcggcgtaa
     4081 aatgccttat ccggcattaa actcccaaca ggacacactc atggcatata ccaccttttc
     4141 acagacgaaa aatgatcagc tcaaagaacc gatgttcttt ggtcagccgg tcaacgtggc
     4201 tcgctacgat cagcaaaaat atgacatctt cgaaaagctg atcgaaaagc agctctcttt
     4261 cttctggcgt ccggaagaag ttgacgtctc ccgcgaccgt atagattacc aggcgctgcc
     4321 ggagcacgaa aaacacatct ttatcagcaa cctgaaatat cagacgctgc tggattccat
     4381 tcagggtcgt agcccgaacg tggcgctatt gccgcttatt tctattccgg aactggaaac
     4441 ctgggtcgaa acctgggcgt tctcagaaac gattcattcc cgttcctata ctcatatcat
     4501 tcgtaatatc gttaacgatc cgtctgttgt gtttgacgat atcgtcacca acgagcagat
     4561 ccagaaacgt gcggaaggga tctccagcta ttacgatgaa ctgatcgaaa tgaccagcta
     4621 ctggcacctt ctgggcgaag gcacccacac cgttaacggt aaaaccgtaa ccgttagcct
     4681 gcgcgagctg aagaaaaaac tgtatctctg cctgatgagc gttaacgcgc tggaagcgat
     4741 ccgcttctac gtcagctttg cttgttcctt tgcctttgca gaacgcgaac tgatggaagg
     4801 taacgccaag attattcgcc tgattgcccg cgatgaagcc ctgcacctga ccggtactca
     4861 gcatatgctg aatctgctgc gcagcggcgc ggatgatccg gagatggcgg aaattgccga
     4921 agaatgtaag caggagtgct atgacctgtt tgtccaggcg gcacagcaag agaaagactg
     4981 ggcggattat ctgttccgtg acggttcgat gattggtctg aataaagata ttctctgcca
     5041 gtacgttgaa tacatcacca atatccgcat gcaggcagtc ggtttggatc tgccgttcca
     5101 gacgcgctcc aacccgatcc cgtggatcaa cacctggctg gtgtctgata acgtgcaggt
     5161 tgctccgcag gaagtggaag tcagttctta tctggtcggg cagattgacg cggaagtgga
     5221 caccgacgat ttgagtaact tccagctctg atggcccgcg ttaccctgcg catcactggc
     5281 acacaactgc tgtgccagga tgaacaccct tcccttctgg cggcgctgga atcccacaat
     5341 gttgcggttg agtaccagtg ccgcgaaggt tactgcggct cctgtcgcac ccgactggtc
     5401 gcaggtcagg tggactggat tgccgaaccg ttagccttta ttcagccggg ggaaattttg
     5461 ccctgttgtt gccgggcaaa aggcgatatt gaaatcgaga tgtgaattgg ttgtagtgcc
     5521 agatacaacg cttatacgtc ttatctggcc tacaacgatt acatggcgta gtaatacgct
     5581 ttcacctgct cccagtccgc tttggggatt ggctccagat attttccagc tggcggaagt
     5641 catgattaat cgctttattg cgacgcaagc ggcgacgact tttctccagg tcaagaaaac
     5701 cagcttctgc tttaccttct gttttcacat agatatggcg aacataacaa cagccatgct
     5761 gacgattaac gctatgcatt ttcttaaacg ccagcgccac tgctttcaac atggcttgcc
     5821 gtacttcgtc agaatagggc gatactgcat gccgggcata ccagtcagca atgctgatga
     5881 accccgccat atcttcagtc accagcaacg cgcgccattc accctcaatt ttcaccgctt
     5941 caccaaaaac gatcttcggc acgatgacac cggcccgttc cagttcttta attaccgcaa
     6001 cctcacggac aatcgttggt cggccgaacg gataacgtac ggaatgaaac agatgatgcg
     6061 tcatgcgctt tacatacagc tttttgccgt tgcgctcgac gcattgcacc ccgctcatac
     6121 cattacggcg atagttaggc tcttcaaccc agtcgccctc tgttgcccac cagtgattaa
     6181 attcgtcgta ctttgctgaa actgccatac cctatcgcct gtcattttta ttaacgacaa
     6241 tgactatagg tggttacctg aggaaaatct taatgaaacg tgtcgtatta atgaatttct
     6301 tttgccgcta ctagtgtgac accgcttttg cccttcattt gcagcgcata gattccacca
     6361 gccacgacca ctaatagtgc gcccagcaga taaaatgtga atttaatcag gtggatacag
     6421 cgatgaattt tattcgacag ggattaggca tcgccttatt accagagtta acgctgaaaa
     6481 ccattgcagg tgaattgtgt tccgttcctc acgaaccaac cttctatcga
//
LOCUS       DAJTZL010000200         6512 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.201, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000200 DAJTZL010000000
VERSION     DAJTZL010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6512
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.201"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            38..250
                     /locus_tag="OO544_003639"
     CDS             38..250
                     /locus_tag="OO544_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6769145.1"
                     /translation="MSNTQKIINTEKYNEWVKKFSEQIFKITGDENVAKNELEPWTPE
                     GNAPNYCWWEVDPVDAANEAMSYHND"
     gene            283..501
                     /locus_tag="OO544_003640"
     CDS             283..501
                     /locus_tag="OO544_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HCR6769146.1"
                     /translation="MTTLFRKEYPQKSRATEFLFLILFIVLMIPISPLIFVWAIGKII
                     EPVTELYTDVVWASFNTLHNKINPYKEN"
     gene            656..889
                     /locus_tag="OO544_003641"
     CDS             656..889
                     /locus_tag="OO544_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001726830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR6769147.1"
                     /translation="MVKPATLTVAFKKARDSVDYNWRANGTPPSFHEQRSLSERLFRE
                     QGVDTKILLGHSNQKMTDIYNDVRGKEWKKLVI"
     gene            complement(922..1560)
                     /gene="ompW"
                     /locus_tag="OO544_003642"
     CDS             complement(922..1560)
                     /gene="ompW"
                     /locus_tag="OO544_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HCR6769148.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDAGSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDYWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTAKYKSGVTTVKDSVRLDPWVFMFSAGYRF"
     gene            1800..1889
                     /locus_tag="OO544_003643"
                     /pseudo
     CDS             1800..1889
                     /locus_tag="OO544_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            1917..2660
                     /locus_tag="OO544_003644"
     CDS             1917..2660
                     /locus_tag="OO544_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0259 family protein"
                     /protein_id="HCR6769149.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            2690..3229
                     /locus_tag="OO544_003645"
     CDS             2690..3229
                     /locus_tag="OO544_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HCR6769150.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            3334..3732
                     /gene="yciA"
                     /locus_tag="OO544_003646"
     CDS             3334..3732
                     /gene="yciA"
                     /locus_tag="OO544_003646"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HCR6769151.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(3772..4491)
                     /gene="tonB"
                     /locus_tag="OO544_003647"
     CDS             complement(3772..4491)
                     /gene="tonB"
                     /locus_tag="OO544_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HCR6769152.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     VKKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALSRNQ
                     PQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPG
                     KPGSGIVVNILFKINGTTEIQ"
     gene            4582..6102
                     /locus_tag="OO544_003648"
     CDS             4582..6102
                     /locus_tag="OO544_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR6769153.1"
                     /translation="MRTVITYLRFSSAIQGAEGADSTRRQNDLFKQWLKKNGDAQIVA
                     SFSDEGLSGYKGKHLTGQFGDMLARIEAGEFPEGTILLVESIDRIGRLEHLETEALMN
                     RILGNGIEIHTLQDGLIYTKDALADDLGISIIQRVKAYIAHQKSKQKSFRVSQKWGQR
                     AKLALAGEQRLTKMVPGWIDPETFKLNEHAETVRLIFKLLLDGESLHNIARHLQSNGI
                     KSFSRRKDANGFSVHSVRTILRSETTIGTLPASQRNDRPAIPNYYEGVVDIPTFNKAQ
                     EILDKNRKGRTPASDNPLTINIFKGLFRCQCGASVHPTGTKNKYAGVYRCNNHLDGRC
                     DVPPLKRKPFDRWMIDNFLGMIDVGNDGESERKIAALQHEVEIVTARIKKATALLLEM
                     DDIDELKIQLKELNQKRTELQTTIDNMRRKASLTDKELPQLKDIDLMTKAGRVECQLI
                     LSKHLKGLTLGKDSVTVTLQNDTEITIPTNPLPLNDGSPIFEIADKELLDIDAYQL"
     gene            complement(6135..>6512)
                     /locus_tag="OO544_003649"
     CDS             complement(6135..>6512)
                     /locus_tag="OO544_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000640586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6769154.1"
                     /translation="ANGLRIQMTFQGVEFRSIVGQTDVSAATGKTATTNDGGATSKKN
                     TGNTTTSLGSPMLTCKELFGYSASELSDEALKARVTCSKSVSVKNGESTFSDAANEQA
                     SKVLKPGNALRKYSVNPNKKGTY"
BASE COUNT         1712 a         1489 c         1550 g         1761 t
ORIGIN      
        1 tgtaaataaa atttaatcct taactggagg tatatctatg tcaaatacac agaaaattat
       61 taacactgaa aaatataacg agtgggtgaa aaaattctct gagcagattt ttaaaattac
      121 tggcgacgag aatgtggcaa aaaatgaatt agaaccgtgg acacctgaag gaaacgcacc
      181 aaattattgc tggtgggagg ttgatccggt tgatgctgca aatgaagcca tgagttacca
      241 caacgattaa tgtcgggagg ccgcccgaaa gggcggtaag aaatgactac attattcaga
      301 aaagaatatc cgcaaaaaag tagggcgaca gaatttttgt ttctcattct gtttatcgta
      361 ttgatgatac cgatatcccc tctaattttt gtctgggcaa tcgggaaaat aattgagcca
      421 gttactgaat tgtataccga cgttgtatgg gcgtcgttca acacactgca caataaaatt
      481 aatccgtata aggaaaactg atatggcaac tttgacaaaa aaagaacggg catggttgaa
      541 cgaattacag gaagttcttg atcgctgtcc tgtggtgtca tcctgccgcg attgcgttct
      601 tagtccgtgg ctattgcacc accatcacgc gaaagggaca gctaagcgcg gcgggatggt
      661 taagccagca acattaaccg ttgcatttaa aaaagcccgg gattctgtgg attacaactg
      721 gcgtgctaat ggcaccccac cctctttcca tgagcagaga tctttatcag agcgattgtt
      781 cagagagcag ggggttgata ccaaaatttt gctaggccat tcgaatcaaa aaatgaccga
      841 tatttacaac gatgtacgcg gtaaggagtg gaaaaaactg gtcatttgat aaccagtttt
      901 gcagaggttt tgcagagaaa attaaaagcg gtacccggca gagaacataa acacccacgg
      961 atccagtcgt accgagtctt ttacggtagt aacaccagat ttgtacttag ccgtggtatc
     1021 gatatccatg taccacactg acatgttaac caaccagtca cggttaatca gataatcaac
     1081 ccccacctgc ccggcagctc cccagtaatc tttcagactg agatcggaaa gccccgcctc
     1141 tttgccatga tcgttaaatc cattatcaaa gaaggtggtg tagttaatac ctgccccaac
     1201 ataaggacgg aatttgctgc cggcatcacc aaaataccac tgcgccatca gtgttggcgg
     1261 cagatgatga acggttgcaa tatcgccggt cgcccgggtg ccgattttat ggcggaacgg
     1321 cgtcgctgcc agtaattcca caccaatgtt gtcggtcgcc atataagtaa acgtcaggcc
     1381 cagttgcgtg ttattggtca cgctgaatcc acccagactt cctaacgtac caccagcacc
     1441 ttctgttgga cgtacggttg cagaacctgc acgcataaaa aattcgcctg cttcatgcgc
     1501 aaaggcactg ccagagagaa gagttgttac tgccaaagcc gccactgtta actttttcat
     1561 atccgctccg tcgttatggt tataaaaacg tgacgaatat acctacattg gagtaacaaa
     1621 tgatcctaca tagatcacat tacatatgat aattcaccct ttattgatct ggattaattt
     1681 agaagttgca ttgagaatcc tggttaacgt gatttaaatc aatttttaga tattttcgct
     1741 acgcaaatat taactcgttc gccacttgcc gcggtttgcc cacgcctgaa ggccagcaat
     1801 tacctccgct ttacaaagat atgcttttcc atacaagacc tgatgctgcc acccgaggct
     1861 taaccagggt acaattgccc gctaattaac acctgcataa aactcaagga gagtgcatgt
     1921 ctatcacggc gcagtccgta taccgtgaca ccggaaattt cttccgtaat caatttatga
     1981 ccattctgtt ggtatcgttg ctatgtgcgt ttatcacagt ggtgttaggg catgttttct
     2041 cacccagtga tgcacagctt gcgcagctca atgacggcgt gcccgttagc ggcagtagtg
     2101 ggttgttcga cctggttcag aatatgtcac cggaacaaca acaaattttg ctgcaggctt
     2161 cagcggcgtc cactttttca ggattaatcg gtaacgccat tctcgccgga ggcgtaatat
     2221 taattatcca gctggtgtct gcgggtcaga gagtcagtgc gctgcgagct attggtgcca
     2281 gtgcgccgat attgcccaag ttatttattc tgatttttct gactaccctt ttagtacaga
     2341 ttggcatcat gctggtggtc gttccgggaa ttatcatggc cattttactg gccctggcac
     2401 cggtgatgct ggtacaggac aaaatgggca tttttgcctc gatgcgtagc agtatgcggc
     2461 tgacttgggc gaatatgcgt ctggtggcac ccgcagtact gagctggttg ctggcaaaaa
     2521 cactgttgct gctttttgcc tcttcttttg ccgcattaac cccggaaatt ggtgccgtac
     2581 tggcgaacac cttgagcaac ctgatttcag ccatattgct catctatctg ttccgcctgt
     2641 atatgttgat tcgccaataa ccttaagcat ttgattacgg aatcgtaaaa tgaagcagtt
     2701 tcttgatttt ttaccgctgg ttgtcttttt cgcgttttac aagatttatg acatctatgc
     2761 ggctactgcg gcgctgatcg tcgccacggc gattgtgctt atctatagct gggttcgctt
     2821 tcgtaaggtt gagaagatgg ccctgatcac ttttgttctg gtggtcgtct tcggtggctt
     2881 gacgctgttc ttccacaatg atgagtttat taaatggaag gttacagtca tttatgctct
     2941 gtttgcggga gccctgttag tcagccaatg ggtgatgaaa aagccgctaa ttcagcggat
     3001 gctgggtaaa gaactcacgc tgccgcaacc ggtatggtcg aagctaaatc tggcctgggc
     3061 tgttttcttt atcctttgcg gtctggcaaa catctacatc gcattctggc tgccgcaaaa
     3121 tatttgggtc aactttaaag tctttggcct gaccgccctt accttaatct tcacattgtt
     3181 aagcggtatc tatatctacc gccacatgcc gcaggaagat aaatcctaac cagactgcca
     3241 gtccttcggg gctggcgctg tatctcctct gttctcctca ggaaaatcat agtagcatcg
     3301 cgcctgtgat tttcctttta agtcggtttt accatgtcta caacacataa cgtccctcag
     3361 ggcgatcttg ttttacgtac tttagccatg cccgccgata ccaatgccaa tggtgacatc
     3421 tttggtggtt ggttaatgtc acaaatggat attggcggcg ctattctggc aaaagaaatt
     3481 gcccacggtc gcgtagtgac tgtgcgggtt gaaggaatga ctttcttacg gccggttgcg
     3541 gtcggcgatg tggtgtgctg ctatgcacgc tgtgtccaga aagggacgac atcggtcagc
     3601 attaatattg aagtgtgggt gaaaaaagta gcgtctgaac caatcgggca acgctataaa
     3661 gcgacagaag cattatttaa gtatgtcgcg gttgatcctg aaggaaaacc tcgcgcctta
     3721 cctgttgagt aatagtcaaa agcctccggt cggaggcttt tgactttcta cttactgaat
     3781 ttcggtggtg ccgttaattt taaacaggat attcaccaca atcccactgc ctggcttacc
     3841 cggctcataa cgccatctgc gcatcgcatt tttcacctca cgctcaaaca tgttcgcagg
     3901 cttggctgag aggatttgta cgttatccac gcgaccatcc ggcgtgacgt caaatttaac
     3961 tttaacctgc ccttcaatgc gcaatgcctg tgctcgtgcc ggatactgcg gctgattacg
     4021 gcttaatgcg cgtggtcctg aagccacact ggtaaccggc ttgctggttg cagccgttgc
     4081 cgtacttgat gtggggcgcg cgggtgccgt attttcaaac ggtgatgccg gacgcgactc
     4141 tacgggtttg acatcgcgtt ttggctgctc ctgtaccttt ttcaccggct tcggttttgg
     4201 cttaggtttc ggcttcggct tttcaatgac caccggtgct tcttttggcg gttcggggat
     4261 cggctcaggt tccggttctg gctctaccac cggctccggt ggcggctgaa cggcttgtgg
     4321 cggttcgaga tcagcaggcg caaccatcgt gacagaaatt ggctgcgcag gcgcaggtag
     4381 ttcaataacc tgatgtaccg aggtatagag cagacccgcc acaacagcac catgaatgca
     4441 gaccgaaagt aacgtcggcc aggggaagcg gcgaggtaaa tcaagggtca ttgtattgta
     4501 gctattcggc attgtagcat tagccataac gtttataagt tcatctttaa atgataatca
     4561 attgcatggg agtttatcac cgtgagaacc gtaataacat acttgagatt ctcatcagcc
     4621 atacaaggcg ctgaaggtgc agattcaacc agacgacaaa acgacctgtt caagcagtgg
     4681 ctgaagaaga atggcgatgc tcaaatagta gcgtctttca gtgatgaagg gttgagtggt
     4741 tacaaaggta aacaccttac tggtcagttt ggtgacatgt tagcccgtat tgaggctggg
     4801 gagtttccag aaggtacaat tctgctagtt gagagtatcg accgtatagg ccgattagag
     4861 catcttgaaa cagaagcctt gatgaaccgc atcttaggca acgggataga gattcacaca
     4921 ttgcaagatg gccttatcta cacaaaggat gctttagccg atgaccttgg cataagcatc
     4981 atccagcgtg tcaaagccta catagcgcat cagaaatcta agcagaagtc tttccgtgtt
     5041 tctcagaaat gggggcaacg tgcaaaactt gcccttgctg gtgaacaacg tcttacaaag
     5101 atggttcctg gatggattga ccctgagact tttaaactca atgaacacgc tgagactgtg
     5161 agattgatat tcaaactgct gctagatggt gaaagtctgc ataacattgc acgtcacctt
     5221 caaagcaacg gtataaagtc gtttagtcgc cgtaaagatg ctaatgggtt ctctgttcac
     5281 tctgtacgca ccattctaag gtcagagaca acaataggca cgttaccagc atcacaacgt
     5341 aatgaccgcc ccgctatacc gaactactac gaaggtgttg tagatatacc aacgttcaat
     5401 aaagctcaag agattctcga caagaatcgt aaaggccgta cacctgcaag tgacaaccca
     5461 ctaacgatta acatcttcaa aggtctgttt aggtgtcagt gtggggctag tgtccatcct
     5521 accggaacaa agaataagta tgctggggtc tacaggtgca ataaccactt agacggtcgc
     5581 tgtgatgttc caccgttgaa gcgtaaaccg tttgaccgat ggatgattga taattttctg
     5641 gggatgattg acgtggggaa tgatggagaa tcagagagaa agattgcagc gttacagcat
     5701 gaggttgaaa ttgtcacagc cagaatcaag aaagctaccg ccctacttct tgagatggat
     5761 gatattgatg aactaaaaat tcagcttaag gaactgaacc agaagcgcac agaactacag
     5821 accacgattg ataacatgag gcgtaaggct tcactcactg ataaggaact accccagctt
     5881 aaagatattg accttatgac taaagcgggt cgtgtcgagt gtcagttgat tttgtccaag
     5941 catctaaaag ggcttacatt gggtaaggat tcggtaactg taacgctaca gaacgacact
     6001 gaaatcacca tacctacaaa cccgttaccg ctaaatgatg ggtcaccaat ctttgaaatt
     6061 gctgataaag agctactgga tattgacgct tatcaactgt aattttcagg ggctatgaag
     6121 ccccttttta tgtctcaata agtgcccttc ttgttagggt tcacagagta ctttcgcaga
     6181 gcattacccg gcttaagcac ctttgaagct tgctcattag cggcatctga gaaggtgctc
     6241 tcaccatttt taacgctaac gctcttagag cacgttacac gggcttttag cgcttcatct
     6301 gataactcac ttgccgaata gccaaaaagc tctttacagg tcagcatagg gcttccaagg
     6361 ctcgttgtag tgtttccggt gttcttttta ctggtagcac cgccatcatt cgtggtagcg
     6421 gtctttccag tagcagccga aacatcagtc tgacctacga ttgaacgaaa ctctacacct
     6481 tggaaggtca tctgaatacg aagaccgtta gc
//
LOCUS       DAJTZL010000201         6428 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.202, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000201 DAJTZL010000000
VERSION     DAJTZL010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6428
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.202"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..98)
                     /locus_tag="OO544_003650"
     CDS             complement(<1..98)
                     /locus_tag="OO544_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic acetyltransferase"
                     /protein_id="HCR6769155.1"
                     /translation="MKVQLYKFTEDKNKTLTFRWTKKHFEFCMDNK"
     gene            complement(308..1732)
                     /gene="patD"
                     /locus_tag="OO544_003651"
     CDS             complement(308..1732)
                     /gene="patD"
                     /locus_tag="OO544_003651"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HCR6769156.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGDVLLEIAEASAEQVDAA
                     VRAADAAFAEWGQTTPKVRAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINILFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHAASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTPFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            complement(1754..2548)
                     /locus_tag="OO544_003652"
     CDS             complement(1754..2548)
                     /locus_tag="OO544_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6769157.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGSVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     LLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            complement(2538..3438)
                     /locus_tag="OO544_003653"
                     /pseudo
     CDS             complement(2538..3438)
                     /locus_tag="OO544_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415959.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(3439..4452)
                     /locus_tag="OO544_003654"
     CDS             complement(3439..4452)
                     /locus_tag="OO544_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6769158.1"
                     /translation="MTYAVEFDNVSRLYGDVRAVDGVSIAIKDGEFFSMLGPSGSGKT
                     TCLRLIAGFEQLSGGAISIFGKPASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVNKKQRHAMAQEALEKVALGFVQQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFIFVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDGLMAEKLCGMTGSFALRPEHIRLNTPGELQANGT
                     IQAVQYQGAATRFELKLNGGEKLLVSQANMTGEELPATLTPGQQVMVSWSRDVMVPLV
                     EER"
     gene            complement(4470..5615)
                     /locus_tag="OO544_003655"
     CDS             complement(4470..5615)
                     /locus_tag="OO544_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR6769159.1"
                     /translation="MSKTFARSSLCALTMTIMTAHAAEPPTNLDKPEGRLDIIAWPGY
                     IERGQTDKQYDWVTQFEKETGCAVNVKTAATSDEMVSLMTKGGYDLVTASGDASLRLI
                     MGKRVQPINTALIPNWKTLDPRVVKGDWFNVGGKVYGTPYQWGPNLLMYNTKTFPTPP
                     DSWQVVFVEQNLPDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGISDPYQLTEEQY
                     QAVLKVLRAQHSLIHRYWHDTTVQMSDFKNEGVVASSAWPYQANALKAEGQPVATVFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMNWSLTPKVQGDVAAWFGSLPVVPEGCKASPL
                     LGEKGCETNGFNYFDKIAFWKTPIAEGGKFVPYSRWTQDYIAIMGGR"
     gene            complement(5860..>6428)
                     /locus_tag="OO544_003656"
     CDS             complement(5860..>6428)
                     /locus_tag="OO544_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HCR6769160.1"
                     /translation="VYSELYFGREKPLPAKAWDRHDGVLHCSSFSKCLVPGFRIGWVA
                     AGKHARKIQRLQLMSTLSTSSPMQLALVDYLSTRRYDAHLRRLRRQLAERKQRAWQAL
                     LRYLPAEVKIHHSDSGYFLWLELPEPLDAGELSLAALTHHISIAPGKMFSTGENWSRF
                     FRFNTAWQWGEREEQAVKQLGKLIQERL"
BASE COUNT         1635 a         1805 c         1518 g         1470 t
ORIGIN      
        1 attttattat ccatacaaaa ctcaaagtgt tttttcgtcc atctgaaggt aagggtttta
       61 tttttatctt ccgtgaattt gtacaattga actttcattg ttaatacttc tacttataaa
      121 ttattagttt aataacgata ctctaaagcc ttatgctaat caactgtgac aggatgaaaa
      181 atcgccatca aacatttagc ctaattttta cgctagttat taatgtaatc attctaatac
      241 ttaaccgcca ttaaactcag aacctaaaac gatagtggcc gtcctataac agccactaac
      301 atctaactta atgtttaacc atgacgtggc ggacgacggt gtaatcctcc agcccataaa
      361 gtgacatatc cttgccgtaa ccagaaagtt tctgcccacc gtgcggcatt tcacttacca
      421 gcatgaaatg ggtattgacc caggtacaac catattgcag ccgtgcgctg acgcgatgcg
      481 ccctgcccac atctttcgtc cataccgaag atgcaagtcc gtactggctg tcattcgccc
      541 agttcaccac ctgttcttcg ttgtcgaagg gcgtaacact cactactgga ccaaatacct
      601 ctttttgcac gatggcatcg tcctgtaatg cgccagccag cagcgtcggc gcatagtaat
      661 agccattacc cttgcgcttt tcaccgccag tgatcacttt gatgtgccct gtcgctttcg
      721 cctcttctac tgccttgctg acgcgttcga gatgcgccag cgagcttaaa ggtccaagct
      781 ccgtagactc gtcatccggc gcaccagatt ttaacgttgc caccgcagca cccagttttt
      841 ccaccagcgt atcgtaaatg cctttttgcg cgtagatccg acaagccgca gtacaatcct
      901 gtccagcatt gtaatagcca aatgtacgta caccttcgac cactgcttca atatccgcat
      961 catcaaaaac aatcactggc gctttgccac caagttccat atgagtacgc ttaatggacg
     1021 acgcggcatg gctgatgatg tgctcgccgg tggcgataga gcccgtcagc gacaccatcc
     1081 gcactttggg atgaccggtc agcggatcac ccaccgtttt gcctctgcca aacagtatgt
     1141 taatcacgcc tgccgggaag atatctttcg ccagctctgc caacttcaac gcggtcagcg
     1201 gggtaatttc tgatggttta agcactacgc agttccctgc cgccagcgcc ggagcaagtt
     1261 tccacgcggc catcatcagc ggataattcc acggtgcgat agaagccacg acccccaacg
     1321 gatcgcgacg gatcatcgaa gtatgacctt caagatattc acctgccgcc agaccattca
     1381 gacagcgcgc cgcacccgcg aaaaagcgaa aaacatcgac aatcgccggg atttcatcat
     1441 tgaacgcact atgcagcggt ttgccacaat tacgggactc cagttcggca aaaacctgac
     1501 cattttcttc gataacatca gccagtttca gcagacattc cgcacgcact ttcggcgtgg
     1561 tttgccccca ttcggcaaat gctgcatctg ccgcgcgcac agcagcatcg acctgctctg
     1621 cggatgcctc ggcaatttcc agtaaaacgt cccccgttgc cggattatag acaggctgtt
     1681 tttccccttc gccgctaacc agttctccgt taatcagtaa cttatgttgc atagcatatt
     1741 cctgtatcag tggttattga ccattgtcgc cttcgcgggt tagccaccag gcccccagga
     1801 tcggcaaggt tgttaccaac ataaccagca gtgccaccac gttagttacc ggtacatcac
     1861 gcggtcgccc aagctgattg agcaaccaca acagtaacgt tcgttcatga cctgccgtaa
     1921 aggtcgtaac gatgatttca tcgaacgaca aggcaaacgc cagcattcct cctgccagta
     1981 acgccgaact gagattcggc aacactacgt agcggaaggt ttgccagcca ttggccccaa
     2041 gatccattga cgcctcaacc agactccagg aggtgcggcg aaaacgggcg atgacattgt
     2101 taaacaccac aactacacaa aaagtcgcat gaccgaccac gatggtgaaa aatcccggct
     2161 ccagattgat ggttttaaag gcggttaata acgccagacc agtgacgctg cccggcagcg
     2221 caatgggcag cagcagtaac agcgaaatgg cgtttttgcc gaaaaagtct cgtcgccaca
     2281 gcgcggcagc tgccagcgtc cctaacacca gcgcaattaa tgtcgccagc gccgccactt
     2341 taagtgacag tgtcacggca tcaagaatat cactacgctg tgctgccacg ctaaaccagc
     2401 gcagcgtcag gccctgcggt ggaaaactaa acgccgcatc ttcagtgtta aacgcatagg
     2461 cggcgattat caggatggga aaatgtagga aaacaacgcc gccccaggcc gccagtttga
     2521 ggaaaaacgg tgcgcgttca gagtgcatcg aacgctccca gacgtttcac gaacgccagg
     2581 tacagtgcga tgagaataat cggcaccaag gtgaatgccg ccgccatcgg catattgcca
     2641 atcgccccct gctgggaata aaccatattg ccgataaaat atcctggagg accaaccagc
     2701 tgcgggacga taaaatcgcc cagagtgagt gagaaggtaa agatagagcc agcggcaata
     2761 cccgggattg ccagcggcag caccacatag cgaaaggttt gtcgtggacg tgcgccgaga
     2821 tcagccgacg cctgcaacaa tgacggcggc aaacgctcaa gcgccgcctg aacgggcagg
     2881 atcatgaacg gcaaccagat atagagaaac accagaaagc gccccagccc ggaagttgac
     2941 agcgtatttc cgcccaccgc aggtaatgta aggaacgcag tcagcagtgg ttccagccca
     3001 agatgttgta aaaaccactg agccacgcca tcttttgcca gcaataacgt ccaggcatag
     3061 gctttaacaa tgtagctcgc ccacatcggc aacattaccg caatataaaa acgctttcat
     3121 tttcccgctg gtatagcgcg ccatatacca cgccattgga aaagccagaa tggcgctggc
     3181 gatagtgact gccacagcca tagtcagcgt gcggagaatg atgtcgtaat tcgccggatt
     3241 aaacagcgca cggatattcg ccagcgtcag ttccggcgtt accgacatgg tgaaatcgtc
     3301 aaaagtataa aatccctgcc ataacagtgt cagcagcgag ccgaaataga caatgccaaa
     3361 ccacattagc gggccaagca gcagtaaaaa cagccccaga cctggacgtg aaggtgattg
     3421 caatacattc atcgccattc acctctcctc aaccagcggc accatcacat cacgcgacca
     3481 ggaaaccatc acctgttgtc cgggcgtgag cgtggcaggc agttcttcgc ctgtcatatt
     3541 ggcctgactc acaagcagtt tttcaccgcc gttcaatttc agttcaaaac gagttgccgc
     3601 gccctgatat tgcaccgcct ggatcgtgcc attggcctgc agttcaccag gggtgttgag
     3661 gcggatatgt tccggtcgca gggcgaagct tcccgtcatg ccacaaagtt tctctgccat
     3721 cagtccatca aaaacattcg atgtaccaac gaacccggca acaaacggcg tgcgcgggcg
     3781 catatagaga tcgcgcgggg aatcgacctg ctcaatgcgt ccattattga aaaccgccac
     3841 acgatcggac atcgataacg cttcgccctg atcgtgggta acgaagataa aagtgatacc
     3901 gagagactgt tgcagttttt tcagttccag ctgcatctgc tcacgcaatt tgagatccag
     3961 tgcgccgagc ggttcatcca acagcaatac gcgcggttca ttcaccaatg ctctggcgat
     4021 agcaacccgc tggcgctgac caccagaaag ttgtgacggt ttacgttgct gtacaaaccc
     4081 caacgccact ttctccagcg cctcttgcgc cattgcgtgc cgctgctttt tattcacgcc
     4141 tttgaccatc agcccatagg cgacattgtc aagaatcgac atatgcggaa atagcgcgta
     4201 gtcctgaaag acagtattca cgtcccgctc ccacggtggc agattgctgg cgggtttacc
     4261 aaagatagag atagccccgc cggaaagctg ttcgaagcca gcaatcaggc gcaggcaggt
     4321 ggttttgccg gagccggacg gccccagcat agagaagaac tcaccatctt ttatcgcaat
     4381 actgacgcca tctactgcgc gcacgtcacc gtacaaccgc gagacgttgt caaactccac
     4441 tgcgtacgtc ataaagcacc ccagcgaagt tagcgaccgc ccataatggc aatgtaatcc
     4501 tgcgtccagc gactgtaggg aacaaacttg cccccttctg ctataggcgt tttccagaag
     4561 gcgattttgt cgaaatagtt aaaaccgttg gtttcacaac ctttttcgcc taataacgga
     4621 ctggctttac acccttccgg cactaccggt aacgagccaa accaggccgc cacatcgccc
     4681 tgcacttttg gcgttaatga ccagttcatc catttgtagg cgcaaaccgg atgtttcgct
     4741 tcgctatgca gcatggtggt atcagcccaa ccggtaacac cctccttcgg gaaaacggta
     4801 gcaacaggct ggccttcggc tttcagggcg ttggcctgat agggccaggc actggaagca
     4861 accacaccct cgtttttgaa atcgctcatt tgcacggtag tgtcatgcca gtagcgatgg
     4921 atcaaactgt gttgagcgcg cagcactttc agcaccgcct ggtactgttc ttcggttagt
     4981 tgatacggat cgctgatgcc caactgcggc tgagtggctt taacgaacaa cgcagcgtcc
     5041 gcaatgtaga tagggccatc ataagcctga acgcggcctt tattgctctt gccgtccggc
     5101 agattttgct caacaaaaac cacttgccag ctatccggcg gcgtcgggaa ggttttagtg
     5161 ttgtacatca gcaggttcgg cccccattgg taaggtgtgc cgtaaacttt gccgccaaca
     5221 ttaaaccagt cgcctttaac cacgcgcgga tcgagcgttt tccagttggg aatcaaggcg
     5281 gtattaatcg gctgcacgcg tttgcccata atcaaacgca gcgaggcatc gccggatgcc
     5341 gtaaccagat cgtaaccccc tttggtcatc agactgacca tttcatcgga agtcgcggcg
     5401 gttttcacat tcaccgcgca gcctgtctct ttttcaaatt gcgttaccca gtcgtattgt
     5461 ttatcagttt gtccgcgttc gatgtatccc ggccaggcga taatatccag tcgcccttcc
     5521 ggtttatcta aattggtagg tggttcggcg gcgtgagcgg tcattattgt catggtgagc
     5581 gcacacaggc tgctgcgggc aaatgtcttg ctcataaggt cttactcctg tcgtaattaa
     5641 ttgttcagcc gccgtgccgt aaaaatatct ccttgtttaa ccatagacgg cggcttgcag
     5701 gcacaccttc gtccctgaga aaatttaatc aggttgtgag ctaccgcgca ttatgccgtc
     5761 tgtaaggtta tcttctggag tatagacaag gggttaggaa atatgactgc tattcgaaat
     5821 tcatattacc caatctaaga ggaattaaat taaacgctat tacagccgtt cttgaataag
     5881 tttgcctaat tgttttaccg cctgttcttc acgttctccc cactgccacg cggtattaaa
     5941 acgaaaaaaa cgtgaccagt tttcaccggt agaaaacatt ttacccggcg caatactgat
     6001 atgatgcgtc agtgccgcca ggcttaattc gccggcatct aacggctcgg ggagctccag
     6061 ccagagaaaa taaccgctgt cactatgatg aattttaact tccgcaggca gataacgcag
     6121 cagtgcctgc caggcacgtt gtttacgttc cgcaagctgg cgacgcaggc gacgaagatg
     6181 ggcgtcgtat cggcgcgtgg aaaggtaatc caccagcgca agttgcatcg gtgagctggt
     6241 ggaaagcgta ctcatcaact gcaagcgttg aattttacgt gcatgttttc cggcggcgac
     6301 ccaaccaata cgaaaaccag gcaccagaca tttcgaaaac gaagagcaat gcaaaacgcc
     6361 atcgtggcga tcccacgctt tcgcaggcag cggtttttcc cgtccaaaat aaagttcgct
     6421 gtaaacgt
//
LOCUS       DAJTZL010000202         6401 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.203, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000202 DAJTZL010000000
VERSION     DAJTZL010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6401
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.203"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..611
                     /locus_tag="OO544_003657"
     CDS             <1..611
                     /locus_tag="OO544_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6769161.1"
                     /translation="IGIDNTYPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDR
                     QLDFIISPQHVSARVQELENLTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRF
                     QNRANFEAMIDANMRPCGINPTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPAT
                     LKYFDAPHRPMYMQEYLYSIRNHRYTATMLQHIAEDRDGTNH"
     gene            complement(608..2086)
                     /gene="scpC"
                     /locus_tag="OO544_003658"
     CDS             complement(608..2086)
                     /gene="scpC"
                     /locus_tag="OO544_003658"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HCR6769162.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPTAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSGLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(2110..2895)
                     /gene="scpB"
                     /locus_tag="OO544_003659"
     CDS             complement(2110..2895)
                     /gene="scpB"
                     /locus_tag="OO544_003659"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HCR6769163.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMMPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(2906..3901)
                     /gene="meaB"
                     /locus_tag="OO544_003660"
     CDS             complement(2906..3901)
                     /gene="meaB"
                     /locus_tag="OO544_003660"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HCR6769164.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPFCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRVLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(3894..6038)
                     /gene="scpA"
                     /locus_tag="OO544_003661"
     CDS             complement(3894..6038)
                     /gene="scpA"
                     /locus_tag="OO544_003661"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HCR6769165.1"
                     /translation="MSNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKQLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSFF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCRTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAADHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            complement(6241..>6401)
                     /locus_tag="OO544_003662"
     CDS             complement(6241..>6401)
                     /locus_tag="OO544_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="specific inhibitor of chromosomal initiation of
                     replication in vitro; binds the three 13-mers in the
                     origin (oriC) to block initiation of replication; also
                     controls genes involved in arginine transport; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ArgP/LysG family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR6769166.1"
                     /translation="EKELASGELIDLTPGLFQRRMLYWHRFAPESRMMRKVTDALLDY
                     GHKVLRQD"
BASE COUNT         1481 a         1663 c         1675 g         1582 t
ORIGIN      
        1 tgattggcat agataatact tatccgacaa ttattttcga tcagttgatt agtctcggag
       61 ataagtatga aggcgtgacc gcccagccag ttgagttcag cgaaaacggc gttatcgata
      121 atctttttga ccgtcagtta gattttatta tctccccaca gcatgtatct gcacgagttc
      181 aggaactgga aaacctgact atcagtgaat taccaccgct gcgattgggc tttctcgttt
      241 ctcgccgtta tgaagagcgt caagagcaag agctgcttca ggagctgccc tggctacaaa
      301 tgcgctttca aaaccgggct aacttcgaag caatgattga tgcgaatatg cgaccttgtg
      361 ggatcaatcc caccattatt tatcgtccct acagttttat ggccaaaatc agcgccgtgg
      421 agcgcggaca ttttctgacg gtgatcccgc attttgcctg gcgactggtg aatccggcaa
      481 cgctgaaata ttttgatgcg ccgcacaggc cgatgtatat gcaggaatat ctttattcaa
      541 tcagaaatca tcgttatacc gccacgatgc ttcagcatat tgctgaagat cgtgacggga
      601 caaaccatta acccagcatc gagccggttg caattaaatt acggtgtaag tcgaagacgt
      661 ggctaagatc gtggtgaata tgtccgccgg gcgcattttc cagatagcga tgcagataat
      721 cccgatacat aggatgtgca caattatcaa tgatagtgcg ggcgcgttga agcggggaaa
      781 gaccgcgcag atcggcgatc ccttgttcgg tgatgatcac tttgacgctg tgttcgctgt
      841 gatcaacatg gctgcacatt ggcacgacgg ttgagatctt gccttcttta gcaatcgacg
      901 gagccataaa gatcgacaga tacgcgttgc gttcaaaatc accgctgccg ccgatgccgt
      961 tcatcagatc gaccccggct acgtgcgttg aatttgcatg cccgtaaata tcaaactcca
     1021 ggccgacgtt cagagcgatg acgcccagac gacggatgat ttccgggtta ttggaaatct
     1081 cctgcggacg caacacaatg cggctggcaa agtaatccat attgtcgtaa atcttgcgca
     1141 gggaatcggc cgagattgtc aggctggagg cgctggcccc gctgattttg ccggtttcca
     1201 gtaaatgcac caccgattcc tgtagcactt ccgaatacat catgaacgga ggaatttccg
     1261 ggttttcccc cagacgcgcc attaccgcat tattgatatt gcccacgcca ctttgcagcg
     1321 gcagaaattc cggcggaata cgcccatgcg ccatttcctg caataagaac gtgaccacgt
     1381 tatcggcaat ctgctggcac atgggatttt gcttatccag catattaccg gcgtcgggca
     1441 agttggtttc cacgacggcg acaatctttt tcggatcgat ttgcacatag cgggtaccga
     1501 cgcgatccat tgcatggaag atcgacacgc tattgcgccg cggtggtgcg ccaggaatca
     1561 caatatccgc cagttctgca acgcgcggat cgtgatagtg attaagttca atgatcactt
     1621 tcttcgcccg cagcagccat gtcggcgcat taccgatccc gctggttaac cagactcgac
     1681 catccggtgc cagtgccgat gcttcgatga cggcaacatc aatgtcgccg aagaaaccgt
     1741 aattgaccat ttgcgccact tcgctcaaat gcaggtcaac gaaactcacc gcgccctgat
     1801 tgatcttttt acgtaaaccg gacgatgttt gatatggcgc acgccaggaa acagcatcgg
     1861 cgtcagaaag tacatcgtca gcggcggcgc tgattgacgc ccccgtcagc aggcgaattt
     1921 gatacggctt tttggcctca tgctgttcgt tagctctgcg ggcaatcgcg gtcggtaagg
     1981 ctttcggcga acccgctggg gtaaagccgc taaatgccac catgtcgtta tgctggataa
     2041 tttctgccgc ttcatcggcg gtcatccttg tccactgagt ttccattttt actccttcgt
     2101 tcgcagggat taatgaccaa cgaaattagg tttacgtttt tcgaggaaag cgttcatccc
     2161 ttcctggtaa tcttcgctgt cataaaccgc gcggcgcatc ccctgaatac gttcaaattc
     2221 atcggagttc atggtgtgcg cttcgcccag tacacgcagc tcttctttga taacagcaat
     2281 ggctaacggc gctttctcag agatgtggtg cgccatttgt aaggtgaaat cttccagttc
     2341 ttccacttcc acaacatggt tgaggatgcc gacagccaac gcgcgctggg cggtgattgg
     2401 cgaagcggta aaaatcagct ctttgacaat gtggaagccc gcgtcgcggg tcaggttgtg
     2461 aatgccgacc aggttatacg ggacgccgag gtttacaggc atcattgaga aggttgaggt
     2521 actggcggcg atgatcagat cggaactcat gatcatttca aatgcgccac cccaaacact
     2581 accttccacc atcgaaatga tcggtttcgg gaatttttgg atcatgcggg tgatttgacg
     2641 caatggatca tcataggaga gcggatcgcg accgccagac ggcagttcgt gaatatcgtg
     2701 acctgcggag aagactttgg atccacttgg cgcccgcaaa atgatacagc gaatttccgg
     2761 ccggttgaga tcgcttaacg cctgcataag atcatcaata aagactttac ttaaggcatt
     2821 aagttttcgt ccatagttaa actcaatgac cgccactttg ttgatagtgg caacgttcac
     2881 atactgataa gacataaaaa ttcctttaat caaaatattg cgtctggata aattcactga
     2941 gctgccgcag gccggtgcgc ggtgagagcg tattgttttt gaccgctaaa agcgtctggc
     3001 ggtaatagcg atcgaaatct tcattcgcga acaggtgatt cagtacttct tcttcggtct
     3061 gcttacgcag ccattccacc gattgttgtt gccgcacttg ttgtaaacga ccactggcag
     3121 ttagcgcggt tttgaagtcg atgatggcgt gccagatctc atcgattcca cgtttttcca
     3181 gtgcgctaca agtcagaacc cgtggctgcc attcgtcgta tttacgtcgc agaatatgca
     3241 gggcactctc gtacatatgc cgggcaatgg cgacattggt atggttatcg ccatcgtctt
     3301 tgttgataac gagcagatca gccacttcca tcagcccttt tttaatgccc tgcagatcat
     3361 cgccgccacc ggcaatttgc aacgagataa aacagtccac catgcgggcg acttccgttt
     3421 ccgactgccc gacgccaacc gtttcgacaa tcactacgtc ataacccgcc gcttcgcata
     3481 acagcattaa ttcccgtgct cgctgactgg caccgcccag atgaccggag gagggtaccg
     3541 ggcgaataaa cgccgcctcg gcacgcgcca ggtcattcat gcgggtttta tccccgagaa
     3601 tgctaccgcc agtgaccggg ctgctgggat cgaccgcaat aacagcaacc tttaatccct
     3661 cacgaatcaa caacatgcca aaggcttcaa gaaaggtact tttccccgcg ccgggtgtgc
     3721 cggtaacgcc cagtcgcagg gcgttaccac agaacggcat aatggcgtca agcagctgtg
     3781 tacttagcgc ctgatgacgt gggtgacggc tttccaccag cgtcatggcc tgggcgagcg
     3841 tggcacgctc accctgacgt aagcggcgaa tactttctgc cagcgtggct tcattaatca
     3901 tgatgctggc ttatcagatt cagtacgtcg cgcacactgt cgagcatagg tgtacctgga
     3961 ccataaatcg ccgccacgcc gcgctcttgc aggaaggcgt aatcctgcgg cggaatgacg
     4021 ccacccgcga ccacgcagat atcttcgcgt ccccattttt tcagcgcttc gaccagttcc
     4081 gggatcagcg ttttatgatc ggcagccagt gaggatgcgc ccactacgtg aacgtcgttt
     4141 tctacggcca ggcgggcgat ctcttcaggt gtagagaaca tcgggcttaa atctacgtcg
     4201 aaaccgagat cggaataggc gctggcgatc actttcgcgc cgcgatcgtg tccatcctgg
     4261 cccatcttag cgatcagaat gcgcgggcga cgaccattgt cggcaaggaa ctgctccgtt
     4321 tgcgcaacaa tggcatcgaa ctcggaggcc gatttctcag actgatgata gctttgcgca
     4381 atcacgccgg taacacactg gcttggcacc agataacggt cgaaagcgac ttccagcgca
     4441 tcggaaattt cacccagggt ggcgcgaacg cgagcggcat taacagcggc agccagcagg
     4501 ttttcgttat gctgtgcggc gtgagtcagg gcgttcaacg cggcggttac ggcggcatca
     4561 tcacgggtgg cgcgaatgcg ttccagcgaa gcaatttgct cgttacgcac catcacgttg
     4621 tcgatctcaa gtacatcggt ttcgtcttcg tgatccagtt tgtacttgtt gacaccaacg
     4681 atgacacgct tgccttggtc gatcagcgac tgttcgcgcg ctgaggcctc ttcgatcatt
     4741 cgttttggca gacctgcttc gatcgctttc gccatgccac cggcttcgtc gatctgttgg
     4801 ataatagctc tggcttgttt gacgatttga tcggtcagcg actcaatgta ataggatccg
     4861 gccagtggat cgacggtgcg gcagagttct gattcttcct ggatgatgat ctgggtgttg
     4921 cgggcaatgc gtgctgagaa atcggtaggc aaaccaagcg cttcgtcaaa ggcgttggta
     4981 tgcagtgact gagtaccgcc cagcgtcgca gccagtgctt caatggtggt gcggataacg
     5041 ttgttatacg gatcctgttc agtcaggctc cagcctgagg tctggcagtg ggtacgcagc
     5101 gccagtgatt tcggatcctg tgcgccaaat ccactgaccg cttcgctcca taaataacgt
     5161 gccgcacgca acatggcgac gttcataaac agatccatgc cgataccgaa gaagaacgac
     5221 aggcgaggag cgaagtcatc aattttcagt ccggcagaga ttgctgcttt gatgtactca
     5281 atcccatcgg cgagcgtaaa tgctacctgc tgcacgcagt tggcacccgc ttcccccatg
     5341 tggtaaccgc tgatactgat ggtattaaat cgcggcatgt tgccggaaca ccaggcgatg
     5401 atgtcggcga taatgcgcat tgacggtttt ggtgggtaaa tataggtgtt gcggcagagg
     5461 tactctttga gaatatcgtt ttgaatggtg ccggtcagtt tatcaggtgt aacaccttgc
     5521 tcttctgcgg cgacgatata aaacgccagt actggtagca ctgcgccatt catggtcatc
     5581 gaaaccgaca ttttatccag cgggatctgg tcgaacagga ctttcatatc ttccacggtg
     5641 tcgatagcga cgcccgcttt gccgacgtca cccgccacgc gtgggttatc ggagtcgtag
     5701 ccacggtggg tggcaaggtc aaacgcaacg gaaagacctt tttgcccggc ggccaggtta
     5761 cggcgataaa aagcgttgga ctcttttgct gttgaaaaac cagcatactg acggatggtc
     5821 cacggttggg cggtatacat agtggcacgc gggccacgaa cgtagggcgg caaaccagga
     5881 agggtacctg tcacctccag attatcgaga tcggcttcgg tatacagctg cttgatggcg
     5941 atcccttccg cggtttgctg aaccagcgag tcgacagttt tctcccgacg gctcaattcc
     6001 ttgttggcaa gctgttgcca ctcctgctcg ttagacatta tttgctccgt aaaacgcaat
     6061 caaaatgagg gtatttgcgc ttacggtgag ctttttacgt gggtttgtcg ccagcaaaaa
     6121 aatgcattca gacgtcgttt tttgccgcct gagatttgtc tattttgtga tgtgattcaa
     6181 ggaatacaaa aagaagtgag gataacgcct gatatgtgct atcaggcatt atttgatgga
     6241 ttaatcctga cgaaggactt tgtgaccata atcgagcaac gcatcagtga ctttacgcat
     6301 catgcggctt tcaggagcaa agcggtgcca gtagagcatc cggcgttgaa atagccctgg
     6361 cgttaagtca atcagttcac cgctggctag ttctttctcg a
//
LOCUS       DAJTZL010000203         6223 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.204, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000203 DAJTZL010000000
VERSION     DAJTZL010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6223
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.204"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            122..952
                     /locus_tag="OO544_003663"
     CDS             122..952
                     /locus_tag="OO544_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418745.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfZ/YjiC family protein"
                     /protein_id="HCR6769167.1"
                     /translation="MSMPLSNALQSQIITDNHFLHHPIVDSEFTHKLKYARMDLENIY
                     LPPLTRGNNHNYDGKSAVEIRKLDISKESWPFNYVTGACRESDGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIMTVTGVANSSGFALAALLNANIELGN
                     DPIIGIEAYPGTAEIHAKMGYKVIPGDENAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(1093..1866)
                     /gene="uxuR"
                     /locus_tag="OO544_003664"
     CDS             complement(1093..1866)
                     /gene="uxuR"
                     /locus_tag="OO544_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HCR6769168.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWRHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(2081..3511)
                     /locus_tag="OO544_003665"
     CDS             complement(2081..3511)
                     /locus_tag="OO544_003665"
                     /EC_number="1.1.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HCR6769169.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLSGIFADDLPQN
                     ADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(3592..4776)
                     /gene="uxuA"
                     /locus_tag="OO544_003666"
     CDS             complement(3592..4776)
                     /gene="uxuA"
                     /locus_tag="OO544_003666"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HCR6769170.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLVQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            5116..>6223
                     /gene="gntP"
                     /locus_tag="OO544_003667"
     CDS             5116..>6223
                     /gene="gntP"
                     /locus_tag="OO544_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HCR6769171.1"
                     /translation="MHVLNILWVVFGIGLMLVLNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIIS"
BASE COUNT         1475 a         1606 c         1578 g         1564 t
ORIGIN      
        1 gtgtagagtt gatatcacta caacagaaac atgcatgtca tttgtttact tctcttttta
       61 ctacagaaaa atttatctat ttttctaaac atcagtgaaa tactcataaa aggtaactat
      121 tatgtctatg ccattaagca atgcccttca gagtcaaatt attacagata atcacttcct
      181 tcatcaccca atagtcgaca gtgagtttac acataaatta aaatatgcac gcatggattt
      241 ggaaaacatc tatcttccgc ctcttactcg tggcaataac cataattacg atggaaaaag
      301 cgctgtcgaa attagaaaac ttgatattag taaagagtcc tggccattta attatgtgac
      361 aggagcatgc agagaatccg atggtattac aacaacagga cgtatgctct atcggaactt
      421 gaaaatcacc tcagctttag acgaaatata tggtggcata tgtaaaaagg cgcatgctgc
      481 cacagagtta gcagaaggtt tacggctaaa tttatttatg aagagccctt tcgatcctgt
      541 tgaggactat acagttcatg aaataacatt aggccctggt tgcaatgttc ctggttatgc
      601 cggaaccacg ataggatata tttcaaccct tccagcttct caggcaaaaa gatggaccaa
      661 tgagcagcca aggattgata tctatattga tcagataatg acggtaacag gggtagcaaa
      721 ttcttccgga ttcgcccttg ccgcattact taatgctaat attgagttgg gtaatgaccc
      781 aataattggt atagaagcct atcccggtac cgcggagatc catgcaaaaa tgggatataa
      841 agttatcccg ggtgatgaaa atgcaccact caaacgtatg actctgcaac cgtcatctct
      901 tccagaactg tttgaattaa aaaatggtga gtggaattat attggtaaat aactaccagg
      961 aagaatgagt actaacattc aggttataca gatagctaaa caataccctg caatttacaa
     1021 agtaatactc attgcgactt cttaatttaa gaaagcgcaa ggaacattta cccttgcgct
     1081 tattataata agtcaggcgt caactttatc cagcggccat gaatcaaaca gatagccatc
     1141 aaaataaata tcgtcaacgt tcgagaattc cagcagacgt tgcttaacgt tttccagatg
     1201 ccgccacatt gccagcttcg ccgctcgggc atcttttttg attaacgcgg cgaggatctg
     1261 tttgtgatcg cccaaccact ctttgcgata caggctgtca tccagatggc tgtgcaactg
     1321 gatccacatt ggattgtttt cccgccactg ccaggactga cggaacagct ccaccagcat
     1381 gctgttatgc gttgcttcgg caatggcgag atggaactgc atatcaccac tttcgctgct
     1441 gcccggcgcg ctggaagcca gctcacgctc ttccagttgc aatgcctgac gcattttgac
     1501 gatatcttcg cgggtagcct gcaaagcggc aaattccgcg atgttacttt ccagtaactg
     1561 acgcgcctgt aacagctcaa aaggaccggc atcattgcag acgttagcat ccggactgtc
     1621 tgtgttgtgg ctgcctgagc tatcaagaac atagataccg gcaccccggc gcacttccac
     1681 cagccctttg atctccagca tgatcagcgc ttcacgtacc accgtcctcg tgacatcaag
     1741 catttctgca atttcacgct ccggcggcag ccgttcgcca ggattgtatg gcgtctttat
     1801 gatcagatcg cggatcatcg ccccgacttc ctggtaaggt ctttgcgcag aggtggcaga
     1861 tttcatggtg ttgtgtccgt taggtgcggt taatcaaatt tttgccggat gcggcgtgaa
     1921 cgccttatcc ggcctacaac gggcaatttt acggcacccg ttaatctcaa actataccat
     1981 tagcactgga agttggcacg tacaggtggg caaaaaaacg aaaactggtc aaccaaatat
     2041 cagtttgatt gaccagtaag tctgtagggt aatcagttag ttacagcgca gccacacact
     2101 cgcgcgcacc gcgttcgcac agctgctgat atgccgccgt caccgcgcca acaaagtcgg
     2161 cattctgcgg cagatcatcg gcaaaaatgc cgctcaggcc cagcagcgct ttcacgcggt
     2221 ctgcgccctg atactgcgcg ttaatcttct ggaactcggc cagcatcggg tcaaccacgt
     2281 caatggcatt accctgttca tccacgccca gtgccaggtg acgccagctg ccgccgtttt
     2341 gcaggtgcag acgcaccgga tccagaagac gctgcggtaa cttctggctg ccgtccattg
     2401 caatctgcca ggtacggtga cgcagagacg ggttgctgaa acgctcgatc agcagcgtcg
     2461 cataggcgtt caggtctgta ccttccggca tcgacagcgt tggcgcttgt tcctgcatca
     2521 tcagggcaaa ggccgctttg cgataatccg gattagtcat ggtgtcggca atggtttcat
     2581 agccgccgag gtaaccgagg tacgccagga aagagtggct gccgttcagc atacgcagtt
     2641 tcatcatttc gaacggcaca acgtctgcaa cgaactgtgc gcccacttta tcccagtccg
     2701 gacgaccatt aacgaagtta tcttcaatca cccactgacg gaacggttcg caggcaatgg
     2761 cgcacgggtc gtaaacaccc agctggtcag caatttcctg taaggtttct ggcgtcgccg
     2821 ccggaacgat gcggtcaacc atggtgcacg ggaaggtgac gttctcttca atccatgccg
     2881 ccagctgcgg atcacgtgcc tgagccagcc ccagaaccgc gaccttcgcc acatgaccgt
     2941 tttcacgcac gttgtcgcag gacatcaccg taaacgcttt cagccctttt tcacgacgca
     3001 gacgcaaggc ttcgacgatg taaccaatcg cggacttcgg cgcagtcggg ttttccagat
     3061 cgtgcttgat cagcgggtta ttgagatcca gctgaccgct tgccgcatcg gcgcaataac
     3121 ctttctctgt gaccgtcagg gagacaatcg ccgtctgcgg acgcgccatc gcgttgagaa
     3181 taccttcaca accgtcgatt tccggatgca gggcttcttt catcgaaccg ataattttca
     3241 gctcggtgct ttctgcgcct ttttccgcca cggtgtacag cagttgctgt tttttcaggt
     3301 tttcaatcag cacacggtcg ttgcctggca tcaggttaac ttcgcagata ccccagtcgc
     3361 tgtcggtact ttccagcaga tgatgggtgt acagcgcctg gtgcgcgcgg tgaaacgccc
     3421 cgcaaccgag atgcacaatg cgtgattcca gacgagaatg atcccatgac gggcgggcaa
     3481 ccggcagatt gctgtcaaca atagtagtca tcgtaaactc cagaatccgt cttcagagag
     3541 tgacggcaag gaagaaggaa ccggcacggc gcgcagccat gccggtgggt atcaacggct
     3601 aaagaaagcg cgctggatcg ccagttcgac accgcgaact tcagccaggc ctttcagacg
     3661 accaattgcg gagtaacctg ggttggtttt cttcttcagg tcgtccagca tctgatgacc
     3721 gtggtccgga cgcatcggga tcaggtcttc tttgccttcc gctttacgac ggtgttcttc
     3781 ttcaacaatc gctttcacca cttcgtacat atcaacgtca ccgttcaggt gcgccgcttc
     3841 gtggaaggtt ttcgggttat cttcacgcat ggtggagcgc agatgggtga agtaaatacg
     3901 cggaccgaac tgcttgatca tatcaaccag atcgttgtca gcacgcacgc cgtaggaacc
     3961 tgtgcacatg gtgaaaccgt tcgccatgct gtttacggta tcaaccatcc actgcatatc
     4021 ttcaatggtg gaaacaatgc gcggcaggcc gaggatcggg cgcggcggat cgtccgggtg
     4081 aacagccata cggacgccaa cttcttcggc aaccggaata atcgctttca ggaagacggc
     4141 aaagttttcg cgcagtttgg ctttgtcgat atctttatac agctccaggt gcttacggaa
     4201 ctggtcgagg gtataccctt cttccgcgcc cggcagaccg gcaatgatgt tacgggtcag
     4261 acgcgctttg tcttcgtcgc tcatagtggc gaagcgttca gcggcctgag caatttcttc
     4321 ttcggtgtaa tccgcttccg cgcctggacg cttcaggata tgcatttcga atgcagcgaa
     4381 ttcgatctgg tcgaagcgca gagctttgga gccgtctggc agcacgtatt cgaggtcagt
     4441 acgggtccag tcgagcaccg gcatgaagtt gtagcacacg gtgcgaatgc cgcactgcac
     4501 caggttgcgc agagtctgct gatagttagc gatccactgt tcatagttgc cagtgtgggt
     4561 tttgatatct tcgtgaattg gcacgctttc tacgacagac cataccaggc ctgcgtcttc
     4621 aacgatcgcc ttgcgtttga ggatctcttc tacggaccat acttcgccgt tcgggatatg
     4681 gtgcagcgcg gtaaccacgc cagttgcgcc cgcctgacgg acatcagcta gagaaaccgg
     4741 atcgtttggg ccgtaccagc gccaggtctg ttccatgatt tttcctcttt cacatagtgg
     4801 gttccacagc aaagagacgt ggagccccgg agttgtttag gtatccggat gaacaaccag
     4861 accgcagccg tggcgttgca tcggatgaga tggcgtataa gttctacctt ttggtaagac
     4921 atgtcaaccc gaaatctgaa attggttaac cacatcgcaa gaatttcact ctcacggttg
     4981 ttgctgcaaa ttgcagcaaa gggtaatcga tggttattga tcagaggatg tgacattcat
     5041 cgcaacaatg gttgaccaat ttacataaca tgtcgcccaa aataacaacg gttcaaccag
     5101 gtaaggtaaa acgttatgca tgtgcttaac attctctggg tggtattcgg cattggcctg
     5161 atgctggtac tgaatttgaa gttcaaaatc aattcaatgg tggctttgct agtggcggcg
     5221 ctgtccgtcg ggatgctggc gggcatggat ttgatgtcgc tgctgcacac aatgaaagcg
     5281 ggcttcggca acacgctggg ggaactggct atcatcgtgg tgttcggtgc ggtcatcggt
     5341 aaattgatgg tcgactccgg cgcggcgcac cagatagcgc atacgctgct ggcgcgtctc
     5401 ggtctgcgct atgtacagct gtcggtaatt atcatcggcc tgattttcgg tctggcgatg
     5461 ttctatgaag tggcctttat catgttagcg ccgctggtta ttgtcattgc cgccgaagct
     5521 aaaatcccgt ttctgaaact ggcgatcccg gcagtagcag ctgccactac cgcacattca
     5581 ctgttcccac cgcagccggg tccggtggcg ctggtgaatg cttatggcgc ggatatgggg
     5641 atggtttata tctatggcgt actggtgacg atcccaagtg tcatctgcgc aggtctgatc
     5701 ctgccgaagt tcctcggcaa tcttgagcgc ccaacgccat cattcctgaa agcagatcaa
     5761 ccggtagata tgaataatct gccctctttc ggcgtttcga ttctggtgcc gctgatcccg
     5821 gcgatcatta tgatctctac caccatcgcc aatatctggc tggtaaaaga tacccctgcc
     5881 tgggaagtgg ttaactttat cggttcctcg ccgattgcaa tgtttattgc gatggtggtt
     5941 gcattcgtac tcttcggcac cgcgcgcggg cacgatatgc agtgggtgat gaacgctttt
     6001 gaaagcgcgg tgaagagcat tgcaatggtg attctgatca tcggtgcggg tggcgtgctg
     6061 aagcagacca tcatcgacac cggcattggt gacaccatcg gcatgttaat gtcccacggc
     6121 aatatctcgc cctacatcat ggcatggctg atcaccgtac tgattcgtct ggcgacgggt
     6181 cagggtgtgg tttcagcgat gaccgccgcc gggattatca gtg
//
LOCUS       DAJTZL010000204         6163 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.205, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000204 DAJTZL010000000
VERSION     DAJTZL010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6163
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.205"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..280
                     /locus_tag="OO544_003668"
     CDS             <1..280
                     /locus_tag="OO544_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HCR6769172.1"
                     /translation="QFPNIDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGD
                     SLEAQQYGIAFPKGSDELRDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            419..1078
                     /gene="glnP"
                     /locus_tag="OO544_003669"
     CDS             419..1078
                     /gene="glnP"
                     /locus_tag="OO544_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HCR6769173.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            1075..1797
                     /gene="glnQ"
                     /locus_tag="OO544_003670"
     CDS             1075..1797
                     /gene="glnQ"
                     /locus_tag="OO544_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HCR6769174.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     DPQVLIKNPPSQRLQEFLQHVS"
     gene            1914..4139
                     /gene="ybiO"
                     /locus_tag="OO544_003671"
     CDS             1914..4139
                     /gene="ybiO"
                     /locus_tag="OO544_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HCR6769175.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEV
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALSHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIQDESARYWSRRLSWLSSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLIIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPTPGATPAEPLPPGEPT
                     L"
     gene            complement(4136..5062)
                     /gene="rlmF"
                     /locus_tag="OO544_003672"
     CDS             complement(4136..5062)
                     /gene="rlmF"
                     /locus_tag="OO544_003672"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HCR6769176.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVAFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            5338..5598
                     /locus_tag="OO544_003673"
     CDS             5338..5598
                     /locus_tag="OO544_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR6769177.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTATIYK"
     gene            5863..>6163
                     /locus_tag="OO544_003674"
     CDS             5863..>6163
                     /locus_tag="OO544_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HCR6769178.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQTNADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNAPG"
BASE COUNT         1427 a         1623 c         1585 g         1528 t
ORIGIN      
        1 tcagttcccg aacatcgata acgcctatat ggaactgggc accaaccgcg cagacgccgt
       61 tctgcacgat acgccaaaca ttctgtactt catcaaaacc gccggtaacg gtcagttcaa
      121 agcggtaggt gactctctgg aagcgcagca atacggtatt gcgttcccga aaggtagcga
      181 cgagctgcgt gacaaagtca acggcgcgtt gaaaaccctg cgcgagaacg gaacttacaa
      241 cgaaatctac aaaaaatggt tcggtactga accgaaataa taacgctaca cctgtaaagc
      301 gcactggcag ttccctctcc cctctgggga gaggattagg gtgaggggcg caaacccgct
      361 ccggggccat taattaccct gaatttgatt atttacacca cggtaacagg aacaacatat
      421 gcagtttgac tggagtgcca tctggcctgc cattccgctt ctgattgaag gtgccaaaat
      481 gaccctgtgg atttcggtcc tcggtctggc tggcggtctg gtaatcggat tgctggcagg
      541 ttttgcacgc accttcggtg gttggatagc caaccacgtc gcgctggtct ttattgaggt
      601 gatccgcggc acacctatcg tcgtccaggt gatgtttatt tatttcgccc tgccgatggc
      661 gtttaacgac ttacgcatcg acccatttac tgcggcggtg gtcaccatca tgatcaactc
      721 cggcgcgtac attgcggaaa tcacgcgtgg tgcggtgctg tctattcaca aaggttttcg
      781 tgaagcagga ctggcgctcg gtctttcacg ttgggaaacc attcgctacg tcattttacc
      841 gctggcactg cgtcgtatgc tgccgccgct gggtaaccag tggatcatca gcattaaaga
      901 cacctcgctg tttattgtga tcggcgtggc ggaactgacc cgtcaggggc aagaaattat
      961 tgccggtaac ttccgcgccc ttgagatctg gagcgccgtg gcggtgttct atctgattat
     1021 taccctggtg ctaagcttta ttttgcgtcg tctggaaaga aggatgaaaa tcctgtgatt
     1081 gaatttaaaa acgtctccaa gcactttggc ccaacccagg tgcttcacaa tatcgatttg
     1141 aacattgccc agggcgaagt cgtggtgatt atcgggccgt ccggttccgg taaatcgacc
     1201 ctgctgcgct gcatcaacaa actggaagaa atcacctccg gcgatctgat tgtcgatggc
     1261 ctgaaggtta acgatccgaa agttgacgag cgcctgattc gccaggaagc aggtatggtg
     1321 ttccagcagt tttacctctt cccgcatctg acggcgctgg aaaacgtcat gtttggtccg
     1381 ctacgcgtgc gtggcgcgaa caaagaagag gcggaaaaac tggcgcgtga gctgctggcg
     1441 aaagtcggtc tggcagaacg tgcacatcac tacccttccg aactttctgg tggtcaacag
     1501 cagcgtgtgg cgattgcccg cgcgctggcg gtgaagccga aaatgatgct gtttgatgaa
     1561 ccgacttccg ctcttgaccc ggaactgcgc catgaagtgc tgaaggttat gcaggatctg
     1621 gctgaagaag ggatgacgat ggtgatcgtg acccacgaaa tcggttttgc cgagaaagta
     1681 gcttcgcggc tgatctttat cgacaaaggc cggattgcgg aagatggcga tccgcaggta
     1741 ttgatcaaga acccgccgag ccagcgcttg caggaatttt tgcagcacgt ctcttaactt
     1801 ctctgcctcc ctttcacccg aaagggaggc acaccagatt cctctcattt aaaatcgccc
     1861 ctcccccagc atctatactt atctttttgc tctgttttct cactggagga gtcatgcggt
     1921 ggatcctgtt catcctcttc tgcctgctgg gcgcacctgc ccacgcggta tccatacccg
     1981 gcgttacaac cacaacgaca acggactcaa cgactgaacc ggccccggaa ccggatatcg
     2041 aacaaaaaaa agcggcctat ggcgcactgg cggatgtgct ggataacgac acctcgcgta
     2101 aagaattgat cgaccagttg cgtactgtcg ccgcgacgcc ccctgcggaa ccggtcccga
     2161 agatcgtgcc gccgacgctg gttgaagagc aaaccgtgct gcaaaaggtc accgaagtca
     2221 gccgccatta tggtgaagcc ctttccgccc gcttcgggca actttatcgc aatatcaccg
     2281 gctccccgca taagccattt aatccacaaa ccttcagcaa tgcgctgagc catttttcaa
     2341 tgttagcggt attagtgttt ggtttttact ggctgattcg cctgtgcgca ctgccgctgt
     2401 atcgcaaaat gggtcagtgg gcgaggcaaa aaaatcgtga gcgcagcaac tggttgcagc
     2461 ttccggcgat gattatcggg gcgtttatta tcgacctgct gttactggcc ctgacattat
     2521 ttgtcggcca ggtattaagc gacaacctga atgcaggcag tcgcaccatc gctttccaac
     2581 aaagtttgtt tctcaacgcc tttgccctga ttgaattttt caaagccgta ctacgcctga
     2641 ttttttgccc aaacgtggcg gagctgcgcc cgttcacgat tcaggacgag agcgcccgtt
     2701 actggagtcg tcgcctgagc tggttaagca gtttgatagg ttatggcctg attgtggccg
     2761 tgccgattat ctctaatcag gtgaatgtac agataggtgc gctggcgaac gtcatcatta
     2821 tgctgtgcat gaccgtctgg gcgttgtacc tgatctttcg taataaaaaa gagattaccc
     2881 agcatttgct caactttgcg gagcattcgc tggccttttt cagcctgttt atccgcgcct
     2941 ttgcgctggt gtggcactgg ctggcaagcg cctattttat cgtgctgttt ttcttttcgt
     3001 tgttcgatcc gggcaacagc ctgaaattta tgatgggtgc aacggtgcgc agcctggcga
     3061 ttattggtat cgcagcgttt gtttccggta tgttttcccg ctggctggca aaaaccatca
     3121 ctctctcgcc acatactcag cgtaactatc cagagttgca aaaacggctg aatggctggc
     3181 tttcggcggc gctgaaaacg gcgcgtattc tgaccgtctg cgtggcggta atgttgctgt
     3241 tgagcgcatg gggattgttc gatttctgga actggctgca aaacggcgcg gggcagaaaa
     3301 ccgtagatat cctgatccgt atcgcactta ttcttttttt ctcggcggtt ggctggacgg
     3361 tgctcgccag tttgatcgaa aaccggctgg cttcggatat tcatggccgc ccgctaccca
     3421 gcgcccgtac gcgtaccctg ctgacgctgt tccgtaacgc gctggcggtg attatcagta
     3481 ccatcaccat catgattgtg ttgtcggaaa tcggcgtcaa tatcgcgcca ttgctggcag
     3541 gtgccggggc attaggtctg gctatctcgt ttggttcgca aacgctggtg aaagatatca
     3601 ttaccggggt gtttattcag tttgaaaacg gcatgaacac tggagatttg gtgactatcg
     3661 ggccgttgac cggcacagtg gaacggatgt cgattcgctc cgtgggcgtg cgccaggata
     3721 ccggggcgta tcacatcatt ccgtggtctt cgataaccac ctttgctaac ttcgtccgcg
     3781 gtattggttc ggtagtggca aactacgatg ttgatcgcca tgaagatgct gataaagcca
     3841 atcaggcact gaaagatgcg gtagcggaat taatggaaaa cgaagaaatt cgcgggctga
     3901 ttattggtga accgaatttt gccgggattg tcggcttaag caataccgcg tttacactgc
     3961 gtgtttcgtt caccacgctg ccactcaaac agtggacggt acgctttgcc ctcgacagcc
     4021 aggtgaaaaa acatttcgac ctggcgggcg ttcgcgcgcc agtgcagact tatcaggtgc
     4081 tgcctactcc gggcgcgacc ccggctgaac cgttaccgcc gggggagcca acgctttaac
     4141 gctggcgatt gacaaaacgg cggcgctgct cgtcgttcat aaaggtccag gcaataaagc
     4201 gactctgctt ttgcccctgg gccatctctt tttttaccac cttcaccgcg cccacgtcgg
     4261 tcagagcacg atacaacggc ggtaagtttt caccacgaga taccagcgag gtaaaccaca
     4321 tcacctgctt cgcgaagcct ttgctctctt caatcatctt tttgataaag gcgacttcac
     4381 caccttcaca ccacaactcc tgttgctgac caccaaagtt cagtgcatcg tctttgttca
     4441 gccccaggtt acggcgttta cgctcactac ctgcccgcgc tgcagcggcg gaatcgtgga
     4501 atggcgggtt acacaaggtc gcgtcgtatt gctcgttttt atggatgatg ccgttaaaaa
     4561 ttgccccact ctctttttgc cgacgcagac gaatggcgcg gttaagcccc ggattagcac
     4621 tgataatcgc ctgcgcactg cttaacgcct ggctgctggt ttcactaccg gtaaaacgcc
     4681 agccatattc atgtacgcca atcagcggat aaatacagtt cgcaccaacg ccgatatcca
     4741 gaatgctggc attcgctgga attgttccgc ttgcttcagc cagtaaatcg gcaaggtgat
     4801 gaatataatc cgcccggccc ggtactggtg ggcagagaaa accgtcgggg atatcccagt
     4861 tcgctacggc gtaaaaatgg gccagcaacg ccttattgag cgccttcacc gccagcggat
     4921 tggcaaagtc tacgctttgc tccccggcgg gtgtaagcgt gaggaattgc ctgagttcag
     4981 gattgacctg acaaagcgtg gcgagatcgt agcggctgtg atgacggttg cgcggatgca
     5041 accccggttt ctgggcggac atagcattct cctttaagca gcggcgtaag atacccgcat
     5101 aacgcgacag ggtaaatatc tggctgcgtt tatcattccc caaaacctta acatggctat
     5161 ggtttatggc aattcatcat aaaaatcagc attatgcatg tcattaattt gtcttatttt
     5221 gtaaaaatat atcgttcaaa aaataagcac atctctgtca attttttgcg tcatttgcat
     5281 cgcgcaggcg acctacactt atcaggcact acccgttgta tttatgagga tgtcattatg
     5341 aaaaagtgcc tcacactact gattgccacc gtcctgagcg gaatctctct cacggcttat
     5401 gccgcgcaac cgatgagtaa cctggacagc ggtcagctac ggcccgccgg taccgtttcg
     5461 gcaactggcg catcaaacct aagcgatctg gaggataaac tggcagaaaa agcgcgcgaa
     5521 caaggcgcga aaggttatgt cattaattcc gcaggcggaa atgaccagat gttcggtact
     5581 gcaaccatct acaaataacc ccaccaacgt aactgcattc gcctctggat gcagttttcg
     5641 cctgttttgt aatcaaaaaa catcaaatat gaactcaatg taaataaatg tatttctttt
     5701 tcgcgcaatg ggtgatagaa aatcgcgcca agtgataatg cttatcaaaa ttattatcac
     5761 ttgcatgaac actatcacgg gattaacagt ggcatcgcat ccgcagagat gctttctcgt
     5821 ggcagtgaaa atttcaacat ataagaaaaa gtcacctgca aaatggaaaa caatcgcaat
     5881 ttccctgcca gacaatttca ttcgctcacg ttctttgccg gtctttgtat tggcatcacg
     5941 cctgtggctc aggcactcgc cgccgaaggg caaactaacg cggatgacac gctggttgtc
     6001 gaagcatcaa cgccttcgct ttatgcgcca caacaatctg ccgatccgaa attctcgcgt
     6061 ccggtagcgg atactacccg cacgatgacg gtgatttctg aacaagtgat taaagatcag
     6121 ggcgcaacca accttaccga cgcgctcaaa aacgcccccg gcg
//
LOCUS       DAJTZL010000205         6120 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.206, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000205 DAJTZL010000000
VERSION     DAJTZL010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6120
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.206"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..358
                     /locus_tag="OO544_003675"
     CDS             <1..358
                     /locus_tag="OO544_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6769179.1"
                     /translation="PGRNVRAKSGLNRSILDQGWYEMRRQLEYKQLWRGGQVLAVPPA
                     YTSQRCACCGHTAKENRLSQSKFRCQVCGYTVNADVNGARNILAAGHAVLACGEMVQS
                     GRPLKQEPTEMIQATA"
     gene            complement(398..1069)
                     /locus_tag="OO544_003676"
     CDS             complement(398..1069)
                     /locus_tag="OO544_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HCR6769180.1"
                     /translation="MCGRFAQSQTREDYLALLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPFFIYRADGQPIFMAAIGSTPFERGDEAEGFLIVTAAADQGLVDIH
                     DRRPLVLSPEAAQEWMRQEIGGKEASEIATNGCVPANQFTWHPVSRAVGNVKNQGAEL
                     IQPVC"
     gene            complement(1176..1409)
                     /gene="yedF"
                     /locus_tag="OO544_003677"
     CDS             complement(1176..1409)
                     /gene="yedF"
                     /locus_tag="OO544_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HCR6769181.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            complement(1406..2611)
                     /gene="yedE"
                     /locus_tag="OO544_003678"
     CDS             complement(1406..2611)
                     /gene="yedE"
                     /locus_tag="OO544_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HCR6769182.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFTALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            2798..3211
                     /locus_tag="OO544_003679"
     CDS             2798..3211
                     /locus_tag="OO544_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR6769183.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEEIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            complement(3245..4732)
                     /gene="amyA"
                     /locus_tag="OO544_003680"
     CDS             complement(3245..4732)
                     /gene="amyA"
                     /locus_tag="OO544_003680"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HCR6769184.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(4810..5175)
                     /gene="fliT"
                     /locus_tag="OO544_003681"
     CDS             complement(4810..5175)
                     /gene="fliT"
                     /locus_tag="OO544_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HCR6769185.1"
                     /translation="MNHAPHLYFAWQQLVDKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLRLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            complement(5175..5585)
                     /gene="fliS"
                     /locus_tag="OO544_003682"
     CDS             complement(5175..5585)
                     /gene="fliS"
                     /locus_tag="OO544_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HCR6769186.1"
                     /translation="MYTASGSKAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSLIQDPV"
     gene            complement(5600..>6120)
                     /gene="fliD"
                     /locus_tag="OO544_003683"
     CDS             complement(5600..>6120)
                     /gene="fliD"
                     /locus_tag="OO544_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HCR6769187.1"
                     /translation="KEVDPGAEAQDKNNGALLGDSVVRTIQSGIRAQFANGASDSAFK
                     TLNEIGIKQDGTTGKLKIDDDKLKKVLNENTASVRELLVGDGKETGITTKIATEVKGY
                     LADDGIIDSAQDSINATLKKLTKQYLSVSASIDDTVARYTAQFTQLDTMMSKLNNTST
                     YLSQQFTAMSNS"
BASE COUNT         1497 a         1608 c         1527 g         1488 t
ORIGIN      
        1 gccggggcgc aatgtccggg caaaatcagg tttaaaccgt tcgatactgg atcagggctg
       61 gtatgaaatg cgccgccagc ttgagtacaa gcagctctgg cgtggcggtc aggtgcttgc
      121 tgttccgcca gcgtacacaa gccagcgttg cgcgtgctgt ggtcatacag cgaaagaaaa
      181 ccgcctgtca caaagtaaat tcagatgcca ggtatgtgga tatacagtga acgccgatgt
      241 aaatggcgct cgtaacattt tagcggcggg gcacgccgtt cttgcctgtg gagagatggt
      301 gcagtcaggc cgcccgttga agcaggaacc caccgaaatg attcaggcga cagcctgaac
      361 gtagcaggga tcctcgtcct tcagggcgtg gaggatgtca acaaacaggt tgaattaact
      421 ccgcgccctg gtttttgaca ttacctaccg cgcgcgatac cgggtgccag gtgaactggt
      481 ttgctggcac acagccgttg gtagcaattt ctgatgcttc tttaccgcca atctcttgcc
      541 gcatccattc ctgtgcagct tctggcgaca gaaccagcgg gcggcggtca tgaatgtcta
      601 ccagaccctg gtctgctgca gcagtgacaa ttaaaaatcc ttcggcttcg tcaccacgct
      661 caaatggtgt gctacctatc gcggccataa aaataggttg tccatcagca cgatagataa
      721 aaaaaggctg ttttttgtcg ccttcttttt tccactcaaa ccagccatcg gcaaaacaga
      781 ttgcccgacc atgttgccag agcggtttaa acatgcgact ggttgcggca gtttctacgc
      841 gggcgttaat cagcggcggt ttatcccacc atccgggagc atatccccag aaaaccggat
      901 ccagatgaag gtgttcatca cgttcactga gcagaagaac tttggttccc ggcgcgacgt
      961 tgtatctgcc aatgggttcg ggatcgtagg gaatatcgcg ttcaatatct tccgcgagaa
     1021 gcgcaaggta atcttcacgc gtttgggatt gggcaaagcg tccacacata gaaacctcct
     1081 gagatcatac tgaaaagaat aggagagggg gcgggtaaaa tctatgtgtc agaggcaaca
     1141 atgttcccgc tgtgggccac agcgggaatg agggattact tctgaattaa ataacgaatg
     1201 gtcggcccgt cttgctgaat atccagcacc gtatagccgt gattacgcgc atccagtgga
     1261 atattattga tcgactgtgg acagtcgctc accacttcca ggatttcccc tttttttaac
     1321 tgcggcatcg cctcaagggt tgcgactgcc ggataagggc agggttcacc caccatatca
     1381 aggcggtaat caggaacgat atttttcatg cgatctcctt agcagtctgc ggtgccgcac
     1441 ggcggaagaa gcgtttttcc cagccgataa tcaacatcag cgcagtaaac aacagcaaat
     1501 atgtcaccag caggccaccc atcgggccaa aggttttcag caggttgatt ttgtcccagt
     1561 cggtggccag cgccggagcg aaatcatccc agtaatacgc cagaatcgtc gagccgatca
     1621 cattgcccag accgacccac cagtagtgca cctggccttc taccgcgcgg tacatccagc
     1681 cggtttcgca gccgcctgcc agcacgatgc caaaaccaaa cagtaaacca ccaattaccg
     1741 cgtttggtcc cgcccacatg attttgggtt caacgcctaa ctgtacgtaa ctgaagatcc
     1801 cgatggcact caccgccata ccgataatga ttgctttcgc catatgggta cgtccggtga
     1861 tccacatatc gcggaacgcc gaagtaaagc agatttgcgc acgttcaatc agtaaaccaa
     1921 agccgacgcc aaacagcatt gccagcccca gtttgggttg gttcatcgcc gtgagcagcg
     1981 cccagcccag catgccgaaa aagaccagca tcccgagacg aaaacgacgc cgcgcctgat
     2041 ccggtttttg cgtcagcggt gaggcggcag aaactttctg cattttcacg ggaatacgga
     2101 agatgggcag cagggtaaag cgcgcgccaa accatgaacc aatggcagtg gcgatggcaa
     2161 agaaccaggc atgcagcgag aactgaggaa tgccggtaaa gaacgccgcc aggttacagc
     2221 ccattgccag acgcgcgcca aaaccggcga taatgccgcc aataatggcc tgcataatgc
     2281 ggatacggct gcgcggcatg cgcagtttga cattgttggc ccacagcgct gcggcaaagc
     2341 agccgccaaa catgccgagg atcatcatcc cgtcgatgcg ggttaatggc gatccttcca
     2401 gatggataat tttaaaataa ccccactctt cagcatggac gccaaacagc tgcaggagct
     2461 gaccgcccca acgggtaaat tcacccgtga cagcccaaaa ggtgccagta atgccaaaat
     2521 agtaagtgga gagaataccc gccgcgatga ccgcagggat gggtgcccag aatttaatca
     2581 accaggcgtg tttgaattgc tgccatgaca tgaatgtagc ctctgaactc agaaggtttg
     2641 aaacaagaat cgcgaatcat acatcatgca aatttatttt cggggtaata attcacaaaa
     2701 ataatagtta gatcaaattt ctctctgaac ctggaaaggc tggcgacatc agggctttaa
     2761 tgccacaatc ctcttttctt ctcatgcagg acgaatgatg aaaaaactcg caattgcagg
     2821 tgcgctcatg ttgctggcgg gctgtgccga agtcgaaaac tataacaatg tcgtgaaaac
     2881 ccccgcgccg gactggctgg cgggctactg gcagaccaaa gggccgcaac gtgcgctggt
     2941 cagtccggaa gagataggca gcctgattgt cactaaagag ggcgataccc tggattgccg
     3001 tcagtggcag cgggtgattg ccgtgccggg caagttgaca ttgatgtctg atgatctcac
     3061 taacgttacc gtaaaacgtg agctgtacga ggttgagcgt gacggtaaca ccattgagta
     3121 cgatggtatg acgatggagc gtgtggatcg cccgacggca gagtgtgccg ccgcgctgga
     3181 taaagcgccg ttaccgacgc cactgccgta atgaaatctc cccggcttgc cgccggggat
     3241 gaatttaaat cacctcttcg ataacccaca cgctgacgct gccgccgttg caaaagaagg
     3301 tcgcttcgcc gttttcgtcg gtcactacgc tctcttgccg attccccaga aaatcacgcc
     3361 aggttttatt gccgtaattc tctcccagat gaatggtttt ttcgccatca tccccgttcg
     3421 acatgaccac cacgcagccg ggatattcgt cagtgccact gcggctaaag gcaatgcagt
     3481 tcggatggtc gaaaaataac gtctgtacac cgtgggcgaa acgctgacgg gcgagaatta
     3541 actcatcaag ctgttcgatt attggcatat ctatcggata ggtttgcccg tcaccaccga
     3601 catcttcgta atgcgcaccg tagaggtccg gatagaacac cgaaggaacg ccattttccc
     3661 gcaacaaaat tagagcatac gccagcggtt taaaccacgg ttcgaccggc gcttcgaggg
     3721 cttgcaacgg ttgggtgtcg tgattggcta cgagggttac ggcgtggaaa ggatcggctt
     3781 ccactaatgt acccgtgaaa atctgcgtca tgtcgtagtc gcgtcccatg cgcgatgctt
     3841 catggaattt catctgcagc ggcgcatcga acagcatggt tttgccttcc acctgatcaa
     3901 tatacgtttg cagtttatcg acttcatgcg accagtactc tgccacaata aacagcggct
     3961 ttggcgcaac ttcctgtacg tgttcgatcc actctttata aaaccacgcc ggaatatgtt
     4021 taaccgcatc aagacgaaaa ccgtcgcatt gtgtttgttc catcacccag cgcgcccagt
     4081 atttaatctc ttccgtcacg gcatgattgc ggaaatcgat attctcaccc atcagataat
     4141 cgaaattacc gagttcatca tcaacctgat cgttccagcc ttcgccggtg tagtcgttaa
     4201 caattttaaa tatgccatct tcatcaggat tttcgatatg gtcgataccg ctaaaacatt
     4261 tgaagtccca gataaactgc gagtattgcc cggcacgggc ggggaaggtg taacgcgtcc
     4321 agccttcgca ctcaatgatt tcttcatcaa tttgcgtacg gtcatcagca tttacacgct
     4381 gcacgcgaat agcttctttt tcatccgccc ccattttgtg gttgactacc acgtccagta
     4441 gcaccgcaat gtcattacgt ttcagagcgt caatggcggc cagcagttgt gctttatcgc
     4501 catatttagt agggatgctg cctttctgat caaactcacc taaatcaaat aaatcatagg
     4561 agtcgtagcc gaccgaatac ccgcccgatg cgcctttata ggcgggcggc aaccagacca
     4621 tattgatacc aatatcatta aaaccgtcgg cgcgctcggc cagttcaggc cagagcttac
     4681 cgccttccgg gtaataccag tgaaaacatt gtaacagcgt gggattacgc attcttcgac
     4741 tccattcaag gggaacatta gaagcgtagc cgtaatcgga ttattcgcga gccatcggct
     4801 cgttaagatt caaaagaggt tatcctgcgg agccagcaca aagccgccct gatctccata
     4861 ggcacttaac actgattttt gcaccgatga ctgaccgacc agtttcgcca gttcatccat
     4921 acgaatctgt aataactgct ttaccttgct ttcgttgtcg agaatcaggc gcagcatcgg
     4981 gcggagctgc tcctgcatcg tggtggacgg ggcaacctct tcagtcagat gtgcaatctc
     5041 ctgcaccgca ttcacatacg ccatttcgct ggcgatgagt tcgtcccatt gttcttccgt
     5101 tgccaggcgt aacatgagct ggcttttatc gacgagttgt tgccaggcga aatacaaatg
     5161 cggtgcatgg ttcatcagac tgggtcctga atcaaagaag gggagagtaa agactctttc
     5221 caggcatcgg caatattgcg cattaatgct tccacttctt cgactgcgga gacatcgttg
     5281 cgtaaattgg cttgcagcaa gcgcctgacc atatagctat aaagagcaat caagttttgg
     5341 gttagttcgt ctttgctctc ttcatcaaga ctcacccgca gcccgttctc aatgatgttg
     5401 atcgcttttg acaaagagac gcctttgcct tgctgattgt tgtcctgcat aaacaggcgc
     5461 gctctaacca gtgcgctcag cactccatca aatagcatgg tgaccagctg ttgctggctg
     5521 gcgctcatta cggcgctttc gacgccaatt tgtgcatagg cttttgagcc gctcgcggta
     5581 tacatgctga cctccgtgat caggaattac tcattgcagt aaattgttgg ctgagatagg
     5641 tactggtgtt attcagtttg ctcatcatgg tatctaattg cgtaaactgt gcagtgtaac
     5701 gcgccacggt gtcatcaatg ctggcactga cggatagata ctgtttggtc agttttttca
     5761 gtgtggcgtt aatactgtcc tgtgcgctat caataatgcc atcatcggca aggtaacctt
     5821 ttacctcggt ggcaattttg gtggtaattc ctgtttcttt accatcgcct accagcaatt
     5881 cacgtacgga tgctgtattt tcattcagga cttttttgag tttatcatca tcaattttca
     5941 gcttacccgt cgtgccgtcc tgcttaatcc cgatctcgtt taatgttttg aaggcactgt
     6001 cgctggcacc gtttgcaaat tgtgcgcgga tgccgctttg aattgtacgt acaacgctgt
     6061 ctccgagaag tgcaccatta tttttatctt gtgcttcagc acctgggtct acttctttat
//
LOCUS       DAJTZL010000206         6052 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.207, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000206 DAJTZL010000000
VERSION     DAJTZL010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6052
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.207"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            65..1321
                     /locus_tag="OO544_003684"
                     /pseudo
     CDS             65..1321
                     /locus_tag="OO544_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000667465.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
     gene            1324..2652
                     /gene="ssnA"
                     /locus_tag="OO544_003685"
     CDS             1324..2652
                     /gene="ssnA"
                     /locus_tag="OO544_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HCR6769188.1"
                     /translation="MLILKNVTAVQLHPAKVQEGVDIAIENDVIVAIGDALTQRYPDA
                     SYKEMHGRIVMPGIVCSHNHFYSELSRGIMANIAPCPDFISTLKNLWWRLDRALDEES
                     LYYSGLICSLEAIKSGCTSVIDHHASPAYIGGSLSTLRDAFLKVGLRAMTCFETTDRN
                     NGIKELQEGVEENIRFARLIDEAKKATSEPYLVEAHIGAHAPFTVPDAGLEMLREAVK
                     ATGRGLHIHAAEDLYDVSYSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDIALL
                     NQRDAFLVHNARSNMNNHVGYNHHLSDIRNLALGTDGIGSDMFEEMKFAFFKHRDAGG
                     PLWPDSFAKALTNGNELMSRNFGAKFGLLEAGYKADLTICDYNSPTPLLADNIAGHIA
                     FGMGSGSVHSVMVNGVMVYEDRQFNFDCDSIYAQARKAAASMWRRMDALA"
     gene            2703..3482
                     /locus_tag="OO544_003686"
     CDS             2703..3482
                     /locus_tag="OO544_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HCR6769189.1"
                     /translation="MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKK
                     IAISLQDLELDWVDWDNGALRIGAMSRLQPLRDARFIPAALREALGFVYSRHVRNQST
                     IGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEDYLACPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVAMTDHDGMRIALDGVASKALRLHDVEKQNLEGNALEQA
                     VANAIFPQEDLRGSVAYKRYITGVLVADLYADCQQAGEEAV"
     gene            3479..>6052
                     /locus_tag="OO544_003687"
     CDS             3479..>6052
                     /locus_tag="OO544_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HCR6769190.1"
                     /translation="MIIHFTLNGAPQELTVNPGENVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIAAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLDVIGKHYPKTDAAKMVQAKPCYVEDCVTADACVIKMLRSPHAHALITHL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AESEEIALEALKLIDVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGAPDTLEDDNS
                     HAAQRGEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADVIIERTYNSTQAQQCP
                     TETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVGMKQHKVHVIKERVGGGFGSKQD
                     ILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKKDGRLTAVKMDF
                     RANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGAPKGNF
                     AITMALAELAEQLQIDQLEIIERNRVHEGQELKILGAIGEGKAPTSVPSAASCALEEI
                     LRQGREMIQWSSPKPQNGDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFIVHSG
                     GADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGNAARL
                     AAENLREKILFHGAQMLGEAVADVQLATPGVVRGKKGEVSFGDIAHKGETGIGFGSLV
                     GTGSYITPDFAFPYGANFAEVAVNTRTGEIRLDKFYALLDCGTPVNPELALGQIYGAT
                     LR"
BASE COUNT         1475 a         1581 c         1624 g         1372 t
ORIGIN      
        1 tcctcagttc cgtattcctg cagagttaat tcagcacgat atcgattttg ttgccgctca
       61 cggcgtgaaa tttgagtatg gctgctcacc cgatttaacc gttgaacagt taaaaaatca
      121 gggcttccac tatgttctga ttgccaccgg cactgataaa aatagcggtg tgaaactggc
      181 gggcgacaac caaaatgtct ggaaatcact ccccttcctg cgtgaataca acaagggtac
      241 agcgctcaag ctgggcaaac atgtggtcgt tgtcggggcg ggtaacaccg caatggactg
      301 cgctcgtgcg gcgttacgcg ttccaggcgt agaaaaagca acgatcgttt accgtcgttc
      361 actacaagag atgcccgcat ggcgcgaaga gtatgaagaa gcgttgcacg acggcgtaga
      421 gttccgtttc ctgaataatc cggaacgttt cgatgctgat ggcaccttaa ccttgcgcgt
      481 tatgtcgctt ggcgaaccgg atgagaaagg tcgtcgccgt ccggttgaaa ccaacgaaac
      541 agtaacactg catgtagaca gcctgatcac cgccattggt gaacagcagg atactgaagc
      601 cctgaatgcg atgggcgtgc cgctggacaa aaacggctgg ccagacgtcg accataatgg
      661 cgaaactcgt ctgactgacg tctttatgat cggcgacgta cagcgcggac catcctccat
      721 tgtcgctgct gtcggaaccg cgcgtcgggc gaccgatgcc atccttagtc gggaaaatat
      781 ccgttcccac cagaacgata aatactggaa caacgtcaat ccagcggaaa tctatcaacg
      841 taaaggcgat atctctatca cgctggtgaa cagtgacgat cgtgacgcgt ttgtcgccca
      901 ggaagccgct cgctgcctcg aatgtaacta cgtttgcaac aagtgtgtgg atgtctgccc
      961 gaaccgcgcc aacgtctcca ttgcagtccc tggcttccag aatagattcc agacgctgca
     1021 cctcgacgct tactgtaacg aatgcggcaa ctgcgctcag ttctgtccgt ggaacggtaa
     1081 accatacgaa gacaaaatca ccgtcttcag cctggcgcaa gactttgata acagcagcac
     1141 cccaggcttc cttgtggaag attgccgggt acgagtacgt ctgaataacc aaagctgagt
     1201 gttaaacatc gacagcgaag gtcagtttaa caacgtacca ccggagctga acgatatgtg
     1261 ccgcatcatc agccatgtcc accagcatca tcattatctg ctgggccgcg tggaggtgta
     1321 atcatgttga ttctgaagaa tgtcaccgca gtgcagttac acccggcgaa agtgcaggaa
     1381 ggcgttgata tcgctatcga aaatgacgtg attgtcgcta tcggcgatgc cctgacgcaa
     1441 cgctaccccg atgccagcta caaagagatg catggtcgga ttgtgatgcc gggaattgtc
     1501 tgctcgcaca accattttta ctcggagctt tcccgcggaa ttatggcaaa catcgcccct
     1561 tgcccggatt tcatctcaac gctgaaaaat ctctggtggc ggctcgatcg cgcccttgat
     1621 gaagagtcgc tctattacag cggactgatt tgttccctgg aagcgattaa gagcggatgt
     1681 acatcggtta tcgatcacca tgcctcgccg gcgtatatcg gcgggtcgct ctccacattg
     1741 cgcgacgcat ttttaaaagt tggcctgcgc gcgatgacct gttttgaaac tactgaccgt
     1801 aacaacggca tcaaagagtt gcaggaaggt gtagaagaaa acatccgttt cgcccgtttg
     1861 attgatgagg cgaagaaagc gacaagcgag ccgtatctgg tggaagcaca tatcggtgct
     1921 cacgcgccgt ttaccgtgcc ggatgccggt ctggagatgc tgcgtgaagc cgtgaaagcc
     1981 acaggccgtg gtttgcatat tcacgctgcg gaagaccttt acgacgtttc ctacagtcac
     2041 cactggtacg gcaaagacct gctggcacga ctggcgcaat tcgacctcat cgacagcaaa
     2101 acgctggtcg ctcatgggct gtacttgtcg aaagatgaca tcgccctact caatcagcgc
     2161 gatgcgttcc tggtgcataa cgcccgttca aacatgaaca accatgtcgg ctacaaccat
     2221 caccttagcg acatccgcaa cctggcattg ggaacggacg gcattggttc ggacatgttt
     2281 gaagagatga aatttgcctt ctttaaacat cgcgatgcgg gtggtccgct gtggcctgac
     2341 agttttgcca aagccctgac taacggtaac gaactgatga gccgcaactt tggcgcgaaa
     2401 tttgggcttc tggaagccgg ttacaaagct gatttgacca tttgcgatta caactcgccg
     2461 acgccgctgc tggcagacaa tatcgccggg catatcgctt tcggtatggg ctcaggcagc
     2521 gttcacagcg tgatggtcaa tggtgtgatg gtctatgaag accgtcagtt taacttcgat
     2581 tgcgattcca tttatgcaca agccagaaaa gccgctgcca gtatgtggcg tcggatggat
     2641 gcgctggcat aaatgacaga tgccctcttc ccgcagggaa gagggttaaa taaggaaaga
     2701 agatgattga acaatttttc aggcccgact ctgtcgaaca ggcgctggaa ctgaagcgcc
     2761 gctaccagga tgaagccgtc tggttcgccg ggggcagcaa actcaatgct acaccaaccc
     2821 gtaccgataa aaagattgcc atttccttac aggatctgga actggactgg gttgactggg
     2881 ataacggtgc actgcggatt ggtgcaatgt ctcgcttgca gccactgcgt gatgcgcgct
     2941 ttattcctgc agcactgcgt gaagccctcg gttttgttta ctcacgccat gttcgtaatc
     3001 agtcgaccat tggtggtgaa atcgccgccc ggcaggaaga gtcagtgctg cttcccgtcc
     3061 tgctagcact ggatgctgaa ctggtttttg gcaacggcga aacgctgtca atcgaggact
     3121 acctggcctg cccatgcgat cgcctgctaa ccgaaattat cattaaagat ccgtatcgca
     3181 cctgtgcgac ccgcaaaatt agccgttctc aggcaggttt aaccgtcgtg acggcagccg
     3241 ttgcaatgac agaccacgac ggtatgcgaa ttgcgctgga tggcgtcgcc agtaaagcac
     3301 tgcgtctgca cgatgtcgaa aaacaaaatc tggaaggcaa tgcacttgaa caggctgtcg
     3361 ccaacgccat tttcccgcag gaagatttgc ggggcagcgt ggcctataaa cgctatatca
     3421 cgggagttct ggtagccgac ctgtatgccg actgccaaca ggctggggag gaagccgtat
     3481 gatcatccac tttactttaa atggcgcgcc tcaggagcta accgttaatc caggcgaaaa
     3541 cgtgcaaaag ctgttgttta acatgggaat gcactctgta cgcaacagtg atgatggctt
     3601 cgggtttgcc ggttctgacg caataatctt taacggtaat atcgttaacg cgtccttgct
     3661 tattgccgca cagttagaga aggcagatat tcgtaccgca gaatctctgg gtaaatggaa
     3721 cgagttaagt ctggttcaac aggcaatggt tgatgttggc gtggtgcagt ctggttataa
     3781 cgatccagct gcagctctga ttatcaccga tcttctcgat cgcatcgccg cacctacccg
     3841 cgaagagatc gacgacgcgc tttctggttt gttcagccgc gatgcaggct ggcaacaata
     3901 ctatcaggtc attgaactgg cggttgcacg taaaaataat ccgcaggcca ccattgatat
     3961 cgctccgact ttccgtgacg accttgacgt cattggcaag cattatccta aaactgatgc
     4021 cgcgaaaatg gtgcaggcga aaccctgcta tgttgaagac tgcgtaacgg ctgacgcctg
     4081 cgtcattaaa atgttacgta gcccacacgc tcacgcactg attactcatc tggatgtcag
     4141 caaagctgaa gccttaccgg gcgtcgttca cgttattact cacctgaatt gccctgatat
     4201 ctactatacc ccggggggtc agagcgcacc ggaaccgtca ccgcttgacc gccgtatgtt
     4261 cggcaagaaa atgcgtcacg tcggcgatcg tgttgctgcg gtcgtcgcag aaagtgaaga
     4321 aattgcgctc gaagcactga agctcatcga cgttgaatat gaagtgctta agccggtaat
     4381 gtcgatcgac gaagcaatgg cggaagatgc gcctgtcgtg cacgatgaac cggtggtgta
     4441 tgttgctggt gcgccagata ctctggaaga cgataacagc catgcagccc agcgcggcga
     4501 gcatatgatc atcaacttcc cgatcggttc tcgccctcgc aaaaatatcg ccgccagtat
     4561 tcatggtcat attggcgata tggacaaagg ctttgccgat gccgatgtga tcattgagcg
     4621 aacctataac tcaacgcagg cacagcagtg cccgactgaa acacatatct gctttacccg
     4681 gatggacggc gatcgtctgg ttatccacgc ctccacccag gtaccatggc acttacgccg
     4741 ccaggtcgcg cgcctcgtgg gcatgaaaca gcataaagtt catgtcatta aagagcgagt
     4801 tggcggcggt tttggttcca aacaggacat cctgctggaa gaagtgtgcg cctgggcaac
     4861 ctgcgtgacc gggcgtccgg tactattccg ctacacccgt gaagaagagt ttattgctaa
     4921 cacctctcgt cacgtcgcga aagtcaccgt caaactggga gcgaaaaaag atggtcgcct
     4981 gacggcagtg aagatggatt tccgcgccaa cactggccct tacggcaacc actcactcac
     5041 cgtaccgtgt aacggaccgg cgctgtcgct gccgttatat ccgtgcgata acgtcgattt
     5101 ccaggtcacc acctactaca gcaacatttg cccaaatggt gcttatcagg gttatggcgc
     5161 accgaaaggt aacttcgcta tcaccatggc attagcggaa ctggctgaac agttacagat
     5221 cgaccaactg gaaattatcg aacgtaaccg ggtacacgaa gggcaagagc tgaaaattct
     5281 cggtgcaatc ggtgaaggta aagcgccgac ctccgttcct tccgccgcca gctgtgcact
     5341 ggaagagatc ctgcgtcagg gtcgcgagat gatccaatgg tcgtcaccga aaccgcaaaa
     5401 tggtgactgg cacatcggtc gcggcgtcgc cattatcatg cagaaatcgg gtatcccgga
     5461 tatcgatcag gctaactgca tgatcaaact ggaatcggac ggcaccttta tcgttcattc
     5521 tggcggtgcg gatattggta ctggtctgga tactgtagtg acgaaactgg cagcagaagt
     5581 gctgcactgc ccaccgcagg acgtgcatgt tatctccggt gataccgatc atgcgttgtt
     5641 tgataaaggc gcatatgcct cgtccggtac ttgcttctcg ggtaacgcgg cgcgtttggc
     5701 agcggaaaat ctacgggaga aaattctgtt ccacggcgcg caaatgttgg gtgaggcagt
     5761 ggcagatgtt caactggcaa cgccgggcgt cgtgcgcggc aagaaaggcg aagttagttt
     5821 cggggatatt gcccataaag gcgaaaccgg catcggcttt ggttcactgg tgggaactgg
     5881 cagttatatc acgcctgatt tcgccttccc gtatggcgca aacttcgctg aagttgccgt
     5941 caacacgcgt acgggtgaaa tccgcctgga taaattctac gccttgctgg actgcggtac
     6001 accggtcaac ccggaattag cattgggaca aatctacggt gctaccctgc ga
//
LOCUS       DAJTZL010000207         6019 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.208, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000207 DAJTZL010000000
VERSION     DAJTZL010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6019
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.208"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(15..614)
                     /locus_tag="OO544_003688"
     CDS             complement(15..614)
                     /locus_tag="OO544_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family protein"
                     /protein_id="HCR6769191.1"
                     /translation="MRKVCAAILSAAICLAVSGAPAWASEHQSTLSAGYLHASTNVPG
                     SDDLNGINVKYRYEFTDTLGLVTSFSYAGDKNRQLTRYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKKHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVAIDIAYEGSGSGDWRTDGFIVGVGYKF"
     gene            complement(685..4182)
                     /locus_tag="OO544_003689"
     CDS             complement(685..4182)
                     /locus_tag="OO544_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HCR6769192.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPIEGPVDGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRTGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVGNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTFKPAYSNNPAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFSGTEPRITCNAWLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPNNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQIVDFSVGAEGLRHVPGDVIEICDDDYAGIRTGGRVLAVNIQTRTLTLD
                     REITLPSSGTTLISLVDGQGSPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVAADDGRERLVSTARTTETTYRFTQ
                     LALGNYRLTVRAVNAWGQQGDPASVSFRIAAPAAPSRIELTPGYFQITATPHLAVYDP
                     TVQFEFWFSEKRIADIRQVETSARYLGTALYWIAASINIKPGHDYYFYVRSVNTVGKS
                     AFVEAVGQPSDDASGYLDFFKGEIGKTHLAQELWTQIDNGQLAPDLTEIRTSITDVSN
                     EITQTVNKKLEDQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMQDGRLYIAGIGAGIEN
                     TPDGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNEVFLKRLTAPTITSGGNP
                     PAFSLTSDGRLTAKNADISGSVNANSGTLNNVTINQNCTIKGMLEATQVRGDFVKAVS
                     KAFPKKVGTWGNTETPNGTVTVTISDDHNFDRQIIIPPIIFNGIAYDDPGSGNNPGGT
                     RYTGYGFEVRKNGVLIASRETKGAIPGSYSAVIDMPSGRGSVTLEFKIFQKGNQGAGN
                     ITDCTVIVTKKAASGISIR"
     gene            complement(4242..4874)
                     /locus_tag="OO544_003690"
     CDS             complement(4242..4874)
                     /locus_tag="OO544_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HCR6769193.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAIRALATQLPAFRQKLSD
                     GWYQVRIAGRDVSTSGLTAQLHETLPDGAVIHIVPRVAGAKSGGVFQIVLGAAAIAGS
                     FFTAGATLAAWGAAIGAGGMTGILFSLGASMVLGGVAQMLAPKARTPTAASTDNGKQN
                     TYFSSLDNMVAQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            complement(4811..5554)
                     /locus_tag="OO544_003691"
     CDS             complement(4811..5554)
                     /locus_tag="OO544_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6769194.1"
                     /translation="MTETESAILAHARRCAPAESCGFVVRTPEEERYFPCVNISGEPE
                     DYFRMSPEDWLQAKMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTGRRFEHGVTDCYTLLRDAYHLAGIEMPDFHREDDWWRHGQNLYLDNLEAT
                     GLYQVPLSAAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRYRAWHASAFTGIYNDLAAASTFV"
     gene            complement(5560..>6019)
                     /locus_tag="OO544_003692"
     CDS             complement(5560..>6019)
                     /locus_tag="OO544_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HCR6769195.1"
                     /translation="VSNLYGMVTGMAEDLQSLVGGTVVRRKVYARFLDAVNFVNGNSD
                     ADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDGAVFPGRIMLANTCTWTYRGDEC
                     GYHGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNVGNFGGFLSINKLSQ"
BASE COUNT         1257 a         1886 c         1513 g         1363 t
ORIGIN      
        1 tattacctgg ctaatcagaa tttataaccg acacccacga tgaaaccgtc agtgcgccag
       61 tcaccactgc cggagccttc ataagcaata tcaatggcca cggattcggt cgggttaaac
      121 tgcacgccag ccccccacgc cagagacgtg ttgctgtggc gaccgtcatc acttccggtc
      181 agcacatcgt gctttttccc cttgttgtca gttacgcgaa gataatcccc ggagaaagtc
      241 gacacacggc tgtaagctac acccgccatc gcatacgcgc tgaaccattc attcacgcgc
      301 acagacggcc ccgccattac gctgaaccag cggttacgaa cggaatcttc atgccagcgg
      361 gtatcgctgt aacgggtcag ctggcgattc ttgtctcctg catagctgaa tgacgtcacc
      421 agccccagtg tgtccgtaaa ctcataacgg tatttcacgt taatcccgtt cagatcatca
      481 ctgccgggga cgttcgtcga ggcatgaaga taccccgcgc tcagcgtgga ctgatgttca
      541 gacgcccatg caggcgcacc ggatacggcc agacaaatgg ctgcggacaa aattgctgca
      601 caaactttac gcataattac ctctcgcttt tctgcaataa aaaaggcgtc attcctgacg
      661 ccctttattg gggttataaa tatttcaacg aatactgatg ccggaagcgg cttttttggt
      721 cacaatcacc gtacagtcgg tgatattgcc tgccccctga ttgcctttct ggaaaatctt
      781 aaactccaga gtgacgcttc ccctgccact cggcatatca ataactgcac tgtaactacc
      841 gggaatggcc cctttagttt ctctggatgc gattaatacg ccgtttttgc gaacttcaaa
      901 accataaccc gtgtatcgcg tgcctcctgg gttatttccg ctccccggat cgtcatacgc
      961 tataccgtta aaaataatgg gcggaataat aatctggcgg tcaaagttat gatcatcgct
     1021 gatggtgact gtaaccgtac cgtttggtgt ttccgtgtta ccccacgtac cgactttttt
     1081 cgggaaggct tttgatacag ctttaacgaa atctcctctg acctgggtcg cctccagcat
     1141 gcccttaatc gtacagttct ggttaatcgt gacattgttg agcgttcctg agttcgcatt
     1201 cacactgcca ctgatatccg catttttcgc cgtcagtctc ccgtctgatg tcagggaaaa
     1261 tgccggagga ttaccgccgc tggtaatggt gggggccgtc aggcgtttca ggaacacttc
     1321 attcatgaat atctgatcgc cctgaccaac aaacatcggc tttgtgttgc cattcgcagg
     1381 attaatcatc gcaatcctgt ctgctgccag cagcacctga ctctgcatgc cgtcaggggt
     1441 gttctcaata ccggcaccga tacccgcgat ataaaggcgt ccgtcctgca tctgctgcag
     1501 cttcactgcc cacatgctgt tcaggttatt atttgtatca acctgaacct tctgtatctg
     1561 ctggatcgct gcactctggt cttccagttt cttattgacg gtctgtgtta tttcattgct
     1621 gacatccgtt atggacgtcc tgatttcagt caggtcaggc gcaagctgac cgttatcaat
     1681 ctgcgtccac agctcctgag ccagatgggt tttccctatc tcgcctttga aaaaatccag
     1741 atagcctgat gcatcatcac tcggctgacc gacagcctcc acgaatgccg atttgccaac
     1801 ggtgttcaca ctgcgaacgt aaaaataata atcatggccc ggtttgatat tgatactggc
     1861 ggctatccag tacagtgccg taccaagata acgcgcgctg gtttcaacct gcctgatatc
     1921 cgcaatccgt ttttccgaga accagaactc aaactgtacc gtcgggtcat aaaccgcaag
     1981 atgcggcgtg gcggttatct gaaaatagcc cggcgtcagc tcaatccgcg acggcgctgc
     2041 cggtgcggca atccggaacg ataccgacgc aggatcgccc tgctgccccc acgcatttac
     2101 tgcccggact gtcagcctgt agttccccag agccagttgt gtgaagcggt aagtggtttc
     2161 cgtcgtccgg gccgtgctga ccagccgctc acggccgtca tccgctgcca cggtcaggcg
     2221 aagcaggaag ctcacgccct tcaccacctt cggcgtgtcc cagcgggcca gcacctggta
     2281 ttccccgctg tctgcggtga cttctgcggt caggtgctgc accgctggcg gcgtgacacc
     2341 atttaccgtg ccgctctggt cgccgtcaaa gtgcgccccg ttatccacga tggcctcttt
     2401 ttccggtaca tgctgcacgg cggtgatggc atacgtgccg tcgtcgttct cacggatact
     2461 cacgcagcgg aacaggcgct ggcgcaacgt cggcagcttc agcccccata cgctgtattc
     2521 agcaacgccg tcaggaacac ggctcacttt caccttcacg ccgtcggtga cggactgaac
     2581 ctccacgctg accggactcc cctgcccgtc aaccaggctt atcagcgtgg tgccggaaga
     2641 tggcagcgtg atttcacggt cgagcgtcag cgtccgggtc tggatgttta ccgccagcac
     2701 gcgcccgccg gtgcggatac cggcatagtc atcatcgcag atttcaatga catcgcccgg
     2761 tacatggcga agcccttcag cacccacgct gaaatccacg atctgcgtct ccagcagttc
     2821 cgttttaatc agccacagcc cggcgcggtg tgcctgcccc cggctggtac agccaaaggc
     2881 atccatcttc gtgacattac gaccgtaacg ggcaatggcc tgcgtatctt caacaagctc
     2941 tgtcgccgtc tcccagccgt tgttcgggtc aatccagttc acctcaacgg cattatggcg
     3001 gtccttcagg gcgctgaagc tgtagcggaa cggcgcgcca tcatccggca tcaccacatt
     3061 actgcggtta taggtccaca ccttatctga tggtcggtcc tgcacgaacg tcagcgtctg
     3121 cccgttccat accggcatac agcgcatcgc cgagcagaaa tcactgagca catcccacgc
     3181 cttacgctgc gtggtcagcc aggcattaca ggtgatgcgc ggctccgtgc cgctaaaacc
     3241 gtccggcact gactggtcgc agtactggcc gatgacatac agcgcccatt tatccacatc
     3301 cgccgcacca agacgtttcc ccatgccgta gcgcgggtgg gtcagcatat cccacagaca
     3361 ccaggccggg ttgttgctgt atgccggctt aaacgttccg tcccagatac cgctgtattg
     3421 ccgcgtctgc gggttatagt tcgacggcac ctgcagaatg cgcccgcgaa gatgataatt
     3481 acggctcacc tgctggctgc cgaactgctc cgagtccacc tgcacgccga ccagtgccgt
     3541 gttcgggtag cactgtttca catcgatgat ttcggtgtat gacgaccaga gcgttttgtt
     3601 ctgcagctgg tctgtggtgc tgtccggcgt catcctgcgc atccgtatat tgaacgggcg
     3661 cggcggcagg ttacccacca ccaccgaggc cagatactgc gaggtggttt tgcccttaat
     3721 ggtgatgtct ttttccgtca cccagccacc attacgctgt atctgaacca gcaggcggac
     3781 ttccgacgga ttcctgtccc cctttgaggt ggtttccacc agtgcctgca cgccgaaggt
     3841 aaaacgcagt cggtcaatgt ttgccgacgt gatggtccgg gtgatcggcg tgtcgtattt
     3901 cacttctgta ccgagcaccg tctcggagcc ggaggattca aatccctccg gcggtgtctg
     3961 ctcctgctca ccggtccgga acaccaccgt gacgccggag atgttggtat tcccctcact
     4021 gtccagcact ggcgtactgt tcagcagcac gctttttaat ccatccaccg gaccttcaat
     4081 cggcccttcg ctgatggcat cgatcacact cagcaactgc gtggacttca ggttgtcctt
     4141 cgcttcgcgc ggagtatgcc ccttactgct gcctttaccc attcctcacg ctccataaac
     4201 gacaaaaccg cccggaggcg gtttcacata aacgtttttc atcagcgacc aatcaccaca
     4261 acctgaccac catccccttc gtctgccgtg ctgatctcct gagagaccac ccgcgacccc
     4321 acgcgcattt caccgtacag aacgggcaga acattgccct gggcaaccat gttatccagt
     4381 gaggagaaat aggtgttctg tttgccgtta tctgtacttg ctgccgtggg cgtcctggct
     4441 ttcggtgcca gcatctgcgc cacaccaccg agcaccatac tggcaccgag agaaaacagg
     4501 ataccggtca taccaccggc cccaatggct gccccccatg ctgcaagggt ggctccggca
     4561 gtaaagaatg atccggcaat ggcggcagcc cccagaacaa tctggaatac gccacctgac
     4621 ttggccccgg cgactctggg aacaatatga atcacagcgc catcaggcag agtctcatgt
     4681 aactgcgccg tcaacccgga cgtgctgacg tcccgcccgg caatccgtac ctgataccag
     4741 ccgtcgctca gtttctgacg aaacgccggg agctgtgtgg ccagtgcgcg gatggcttca
     4801 gctcccgttt tcacacgaag gtcgatgcgg cggccaaatc gttgtaaatc cccgtaaagg
     4861 cagatgcgtg ccatgcccgg taacgccaga gggagtgtgt gcgtcgctgc catttgtcgg
     4921 tatacctctc tcgtttgctc agttgttcag gaatatggtg cagcagctcg ccgtcgccgc
     4981 agtaaatagc ggcatgattc ggcaccgatg aaccaaaaca gcacagcagc acatcgcccg
     5041 gctgcgccgc tgacaacggc acctgataca gccctgtggc ctccagatta tccagataga
     5101 gattctgacc gtgacgccac cagtcatcct cgcgatgaaa atccggcatc tcaatccccg
     5161 ccagatgata agcatcccgt aacagcgtgt aacagtccgt caccccgtgc tcaaagcgcc
     5221 gcccggtgag atgcggcaca cagcggaact tgtgaatcgc cccccggcag accagccacc
     5281 acggcaaatc actctgcacc tgcagccgcc ggtcggcctc actcagccag ggcagaccac
     5341 cggggtgact gtggaccagc gccacaatct caccctgcat tttggcctgc agccagtcct
     5401 ccggcgacat ccggaaataa tcctccggtt caccggagat attcacgcag ggaaaatatc
     5461 tttcctcctc cggcgttctc accacgaagc cgcacgactc cgctggcgca catcgccggg
     5521 cgtgcgccag aatcgctgat tctgtctctg tcatgggatt tactgcgaaa gtttgttaat
     5581 ggaaaggaag ccgccaaagt tgccgacgtt attgcgaaac ttacagccac tcaggcattt
     5641 gctgcattta tccttcgtga tatcggacgt cggctggtca tattcatccg cgaccgccgg
     5701 accgtgataa ccgcactcat cgccgcgata ggtccaggtg caggtgttgg ccagcatgat
     5761 tcgtcccgga aaaacagcgc cgtccgtttc cgtcggcgtg gacagtacaa aagaggcact
     5821 gaccgcgctc agttcgctgc actgctcgat gcgccagcgg ctgatcacct cctgctccgg
     5881 atcggcatcg ctgtttccgt tgacgaagtt caccgcatcc agaaaacggg cgtaaacctt
     5941 acgccggacc accgttccgc cgaccagact ctgcagatct tccgccatcc cggtgaccat
     6001 accgtacagg ttagaaacc
//
LOCUS       DAJTZL010000208         5973 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.209, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000208 DAJTZL010000000
VERSION     DAJTZL010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5973
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.209"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..202)
                     /locus_tag="OO544_003693"
     CDS             complement(<1..202)
                     /locus_tag="OO544_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HCR6769196.1"
                     /translation="MSIEHILIGKHHGGSDYWRTPLLLFKNLHREFGFTLDGAATEHD
                     TLLPRFTNDISRQSWVSEKVFCN"
     gene            718..1287
                     /locus_tag="OO544_003694"
     CDS             718..1287
                     /locus_tag="OO544_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR6769197.1"
                     /translation="MRTFLYARVSVDDLTTQNQVEAVKRAGYEVRPSRVIEEVISGAT
                     PAMDRPQFVKLVDKLEEGDCLVVTKIDRLGRDNIDVQKTVTMLLGMGVKLVCLDLPVQ
                     DLSSAEGKLILQLFSTFAEFELNRIRERTREGLERAKAQGKVLGRPVAKNTTEAVLEH
                     KAKGLSQSQVAKLLNLSVRTVSRHWTKTL"
     gene            2015..2188
                     /locus_tag="OO544_003695"
     CDS             2015..2188
                     /locus_tag="OO544_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HCR6769198.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGQHSGGNFKNDPQRASEAG
                     KKGGKSSHGKSDN"
     gene            complement(2446..3774)
                     /gene="rutG"
                     /locus_tag="OO544_003696"
     CDS             complement(2446..3774)
                     /gene="rutG"
                     /locus_tag="OO544_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HCR6769199.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSHAAWFGLPHFSTPAFNSQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(3795..4289)
                     /gene="rutF"
                     /locus_tag="OO544_003697"
     CDS             complement(3795..4289)
                     /gene="rutF"
                     /locus_tag="OO544_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization flavin reductase protein
                     F"
                     /protein_id="HCR6769200.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPSGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSYHALM
                     RPAC"
     gene            complement(4300..4890)
                     /locus_tag="OO544_003698"
     CDS             complement(4300..4890)
                     /locus_tag="OO544_003698"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HCR6769201.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLRELYAPIKWGP
                     TSANCSPARIVFIRTVEGKERLHPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            complement(4900..5700)
                     /gene="rutD"
                     /locus_tag="OO544_003699"
     CDS             complement(4900..5700)
                     /gene="rutD"
                     /locus_tag="OO544_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HCR6769202.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIEHYAVVGHALGALVGMQLALDYP
                     ASVTVLISVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPSACSS
                     ELHAALPDSQKMVMRYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            complement(5708..>5973)
                     /locus_tag="OO544_003700"
     CDS             complement(5708..>5973)
                     /locus_tag="OO544_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyrimidine utilization protein C"
                     /protein_id="HCR6769203.1"
                     /translation="VLFADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKN
                     YAAINEIYAEFFPGDKPARFCIQCGLVKPDALVEIATIAHIAK"
BASE COUNT         1607 a         1536 c         1446 g         1384 t
ORIGIN      
        1 ggttacagaa gaccttttca ctaacccatg attgacggct tatatcattg gtgaatcgtg
       61 gtaacagcgt gtcatgctca gttgcagcac catctagcgt aaaaccaaat tcacggtgta
      121 agttcttaaa taacaacaat ggagttctcc agtaatcaga acccccatga tgtttcccga
      181 ttaaaatatg ctcaattgac ataagaaata aaaaatttta ttgcaatcac tcttgacaag
      241 gtttactctc ttttaagata agagatactt aacaggcttt ctaaacggat atcctttctg
      301 gaatatctat ctcttatctt aaaagtcttt acagatactt taaagatatc tgtattaaat
      361 ctgtacagta acttttcaga atctgaaaat ttttataaaa tcgtagtagc acctcttcaa
      421 attccccttc taaaaatctt cgaaaatatc tgacagatgt ctgtagctgc taagtacccg
      481 tcacaccagc tatgtgaccc cctttgcaaa aaagccaaat atcattgact ttctcggtga
      541 attgctataa aggcatctat ctaggtcttt aactgtataa atacacatga caacaaacgc
      601 ccgttaagtg tctatataaa aaggtagctg tagctataga caacaattgt gataattgat
      661 ttaatggcta tagacaagca agacagacct ttgttgacta tatagagaga taagaacatg
      721 cgtacattcc tttatgctcg tgtatcagtt gatgacctca ccacacagaa ccaagtagaa
      781 gctgttaaac gtgctggtta tgaagtcaga ccttcacgag taattgagga agttattagt
      841 ggggctacac cagctatgga cagaccacaa tttgtaaagc tggtggataa gctggaagaa
      901 ggtgattgtc ttgtagtgac caagattgac cgcttaggaa gggataacat tgacgtacag
      961 aagaccgtca caatgttact tggtatgggt gttaagctgg tctgtctaga tttgcctgtc
     1021 caagaccttt caagtgctga aggtaaactt attcttcaac tattcagtac ctttgcagag
     1081 tttgaactta accgcatcag agagcgaacc agagagggct tagaacgagc taaagcacaa
     1141 ggtaaggtgt tgggtcgtcc tgtagctaaa aacactacag aggccgtttt agagcataag
     1201 gctaagggat tgtcacaaag tcaggttgca aagcttctga atctctctgt gagaacggtt
     1261 agccgtcact ggacaaagac actgtaaaaa aaacttagta gcatcttcat agcctccttc
     1321 atggggactt ttttgtgtct gtaacatatg gtgaccaaac atctatatat cgttatatag
     1381 ccctgtatat agcactatat atagtgtctg gagagggacg actaacaccc tgcatagttc
     1441 cctgaaaagg ggcttcacag attctctata tatttcaata agttaggata aaaagggact
     1501 ataccgatac tgcatagaat ctgtgtagga ttgcatagtc ataggggttg gaacaagccc
     1561 aactacacct gataaagcct gttattaaca ggtagttgaa tgtacgtgtt tagctatgta
     1621 aaagcaccag aactttagcc atttctaacc actcctcgtg ttatctctat tccgtagcga
     1681 ttcgctacca cttatttaaa gataagacgt ccttttcaga gtgcaaattt cacaaccact
     1741 tatttgattt ataacaactt tcacaagcac gtaattttgt cgcaaaatga cacattttta
     1801 tctcatcgcg tttttttaat cataagagcg gcttatggat aattattgga gatgatatct
     1861 attctcgcta agaagctgtt gcaggatatt accaaacgcg ggtctacccg cgtcagttca
     1921 ctaagcttag tcccacgtag cgaaaatatg gcagccgcca tacgccgcgt taattctatg
     1981 caatatgatg tctataccca gacggaggtc agtaatggca aaccatcgag gcggttccgg
     2041 caattttgca gaagaccgcg aaagagcatc agaagcaggt aaaaaaggtg gacagcacag
     2101 cgggggtaat ttcaagaatg acccgcagcg cgcatctgaa gcaggtaaaa aaggtggcaa
     2161 gagcagtcac ggcaaaagcg acaactagcc gggctaatca atgacgaatg catttttgtc
     2221 tgtagctcgt caaaaagcca tcaccgccgg ttacccggtg gttgatactg atgacaaatg
     2281 taagcttgcc tgatgcgcga tgcttatcag gcctaccaga agattgcaat atattgaatt
     2341 tgcactgttt tgtaggccgg ataaggcgtt tacgccgcat ccggcatgaa caatgcgtac
     2401 gttgtcaaca atctgcaccg ccgggtaacc ccggcggttt tctgtttatg gctcctgatg
     2461 aacaacttct ggcggtggaa cgtcaaccaa ccttctactt aacaacgcat tgagtaaaat
     2521 cgcgccaaag gtggctgtac cgatccctcc caacgtaaaa ccgcccagcg tgagagcaaa
     2581 atcacccgcg cccagcacta aggttactgc gaccataatc aaattaccgt tctggcttaa
     2641 atcgacacgg ttttgtaccc agatccttgc gcctgcgacg gcaatcagcc cgaacacaac
     2701 aattgatgca ccaccaatca ccgcggccgg aatggtatgg atcagcgcgc caaatttcgg
     2761 cgaaaagccc aacagcatgg cgatgacggc agcagcaaca aacaccagcg tcgagtagac
     2821 ttttgtcacg gccatcacac cgatattttc agcataggtg gtgacgccgc taccgccgac
     2881 agagccggaa agcatcgttg ccagaccatc accgacgaat gctcgcccca tatacgggtc
     2941 catattgcgt ccggtcatcc cggcgaccgc cttgagatga cctaagtttt ccgccaccag
     3001 aatcaccgcc acgggcgcaa tcagcatcat tgcctgacta ttaaaagcag gagtggaaaa
     3061 atgtggcaaa ccgaaccagg cagcatggct gacgagagta aaatcgacgg cttttcccag
     3121 ccctaaaacg ttggtcatca cgccatacag cagacaggcg acaattaaac ctacgagaat
     3181 caataaccgc tggatcatgc cacgggtaaa caccgccacc agcccaatac acagcaccgt
     3241 cattaccgcc atccagctat caaaggctga agccgataca cttttcactg cgataggcgc
     3301 taagttcagg ccaatcgcca tcaccaccgc acccgtcacc accggcggca ttagtcgttc
     3361 aatccagcgt gtaccaattt tcatcaccac caggccaatg acggtataaa ccagcccaca
     3421 ggcgataatc ccgcccagtg cgatgctgat attcgggtta atgccctgac cgttaaagcc
     3481 cgtcgcggca atcaccacgc cgacaaaagc cgcgctggag ccgagataac tggggacgcg
     3541 cccgccggta ataaagaaaa acagtaacgt gccgatcccc gacattaaaa tggaaagatt
     3601 gggatccagc cccatcagaa tcggcattaa caccgtcgcg ccaaacatcg ccaccgcgtg
     3661 ttgaacgccc attactgccg tctgagcaaa cggcaatcgt tcatccggcg cgaccacgcc
     3721 gctctctgta gaggtcgatt ttaactgcca gtgaggaaaa ccgaacattg ccatcagctg
     3781 tctccttaag gaggttaaca agcagggcgc atcagcgcgt gataactgcg atcgaaccac
     3841 accagcccgt agggtgtggt gtgacgatga atcgcttcga tggcgcaaaa cagaatgtcg
     3901 tgggtgccga cgctcaccac ctggctgatg cggcagtcaa acgaaaccag agcctcttcc
     3961 agttgcgggc atccggtcac acccgtctgc cagcgggcgg cggcaaagcg gtgttccatg
     4021 ggcgttttgc cgccaaaaag atttgaaagc ggctcctgcc cggcgctcag tgtatttaca
     4081 cacagcgttc gattttcatt gaataccggc cagacggacg ccccacgatt caggcacacc
     4141 agtaatgtgg gcggcgtgtc ggtcacgctg cagacggcgc tggcggtgaa cccggcgcgc
     4201 ccggatggac cgtccgtggt gatgatattg accgccgcgc ccatacagga catcgcatcg
     4261 cgaaaggttt gttgatcaac tatgttcata gtttgctcct tacaacagcc cgcaggcttc
     4321 ttcaaaggac agacgtggca ggcgcgcaaa aagtttgctg ctatcgccat agccgatatt
     4381 aatcagcaga ttgctcttca gcgtgctgcc cgcaaaaaag gcgtcgtcaa cgtgttgacg
     4441 gtcaaaaccc gacatcgggc cggtatccag tcccagcgca cggcaagcga cgatcagata
     4501 ggccgcctgc atggagctgt tgcgaaacgc tgtttcttcg gcaagttgtg ggctggaggt
     4561 aaaccaactg cgggcatcac cgtggggaaa cagtagtggt aaccgttcat aaaattcact
     4621 gtcccaggca acgatagcgg tgacgggcgc ggtcagggtt ttttgtagat tgccgctgga
     4681 aagcgccggg tgcagacgtt cttttccttc tacagtgcgg ataaacacga tccgtgccgg
     4741 agaacagtta gctgatgtcg gcccccattt tatcggggca taaagctccc gtaacgtctc
     4801 atcgctgacg ggtgtctccc gccagccgtt gtgagtgcgg gcatcggtga acagggtgct
     4861 aagcgcacct gggctaacgg cttcgttcat agcaattcct tacagggcgg cttcacggtg
     4921 atgtaacagg ctggcaagcc cgttgagtaa cagagcatta aacgtttcgg ggtcggtcac
     4981 gttgcaggcg tgtccgccat agcgcatcac cattttctgg ctatcgggca gggcggcatg
     5041 aagctcactg gaacatgctg atggcaccag cagatcatca ctggcgcaga tgatttgcac
     5101 cgggcagcgg atgcgatccg catggtgact aaagtcagcg cgtttgaggg cgttaagtcg
     5161 acgcagtaaa ttatttttgc cctgaaaatg cgccagtgcc agcgcgtctt ctgcctcaag
     5221 gcgaggtgcg cgggccgcca tccagtcggc gggatagagg aacaacggct gcgcttccac
     5281 ccatgcctgc gcgccgccgc tatacagtaa tcgttcgcga acctgaaaac agcggcgcgt
     5341 atgggcgttt attcgtagcc agccgttaac gctgatcagc acagttaccg acgcgggata
     5401 atccagcgcc agctgcattc ccaccagcgc accgagcgca tggccgacca ccgcgtaatg
     5461 ctcaatccct gcggctacca gcgcctgatg cagttccgct gccatctggg cgatactgta
     5521 atcttctgcc agcgtgtcgg gattattgcc ggtgccgcgc tggtcgtaac agactacctg
     5581 atactcctgc tccagcaccg ccagttgcgg taaccagtaa ctgccgctac cgccaagacc
     5641 cgaaatcaac accactacgg gcgcatcagc ataaggggga ggtgagagtg aaagtttcat
     5701 cgcggcctca cttggcgata tgcgcaattg tggcgatttc caccagcgcg tcaggtttta
     5761 ccagtccgca ctgaatgcag aatcgcgccg gtttatcacc cggaaaaaac tcggcgtaga
     5821 tttcgttaat cgcggcgtaa tttttccagt cggtaataaa gatgctgttg aaggtcacat
     5881 ccgccatcgt gccacccgcc gtctcgatca ccttgcggat agtttccaga acgtggcggg
     5941 tttgcgcctt tgggtcatcg gcaaacagca cgt
//
LOCUS       DAJTZL010000209         5934 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.210, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000209 DAJTZL010000000
VERSION     DAJTZL010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5934
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.210"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            292..368
                     /locus_tag="OO544_003701"
     tRNA            292..368
                     /locus_tag="OO544_003701"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:326..328,aa:Arg,seq:tct)
     gene            complement(413..1174)
                     /gene="envY"
                     /locus_tag="OO544_003702"
     CDS             complement(413..1174)
                     /gene="envY"
                     /locus_tag="OO544_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HCR6769204.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKNLTCVKPWSRLATPVIACHSRTPEVFRLAA
                     NHSKQQPSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENISYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYGLTPLNYLAKQRQKVMW"
     gene            complement(1357..2247)
                     /locus_tag="OO544_003703"
     CDS             complement(1357..2247)
                     /locus_tag="OO544_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HCR6769205.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRLQGFDTLVLQWTRYGDAFTQPEQRTLLFKRAAAAQQAGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGITPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDTLYFSLRYLPVAHGILEY"
     gene            complement(2248..5220)
                     /gene="nfrA"
                     /locus_tag="OO544_003704"
     CDS             complement(2248..5220)
                     /gene="nfrA"
                     /locus_tag="OO544_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HCR6769206.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDARLERSLAAIPVEVKSVTTVEELLAQQKACDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLNEN
                     KPLFTTDAQEKSWLYLLSKYSANPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYAVSVATRNKAESLRLARLLYQQEPANLTRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARVSQTLMARLASLLESHPYLATPAKVAILSKPLPLAEQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPSAWQHRAVAYQAYQVEDYATALAAWQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRGLGSNALYWWLHAQRYIPGQPELALNDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELEPNNSNIQAALGYALWDSGDIAQSREMLEQAHKRLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRR
                     WTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRIGRNMLLEGDLLSVY
                     SRVFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            complement(5207..>5934)
                     /gene="nfrB"
                     /locus_tag="OO544_003705"
     CDS             complement(5207..>5934)
                     /gene="nfrB"
                     /locus_tag="OO544_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl transferase family protein"
                     /protein_id="HCR6769207.1"
                     /translation="LDTALRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESI
                     DAWQIPSSLIAEMPASVALHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVR
                     YFIVLRGQIVTGLRHWYARRRGHDPRAMLYNAVQHQWLTEQQAGEIWRQYVPHQFLFA
                     EILTTLGHINRSAINVLLLRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQ
                     SLLLKAGLNTEQVAQLESENEGE"
BASE COUNT         1347 a         1559 c         1551 g         1477 t
ORIGIN      
        1 ttctgatgat aggatgagta tccataatga tcaccgacgt tggtttcata gttaccagtc
       61 tcataggagc ggacaatttt ccgttagggc ggtaaattgt actttgatac atgaaaatac
      121 gggttttctt gattcaaacg cgcagcggtg tgcatttgtt tgccgctata gcgtaataaa
      181 tcagaaaatc agacgcggtc gttcacttgt tcagcaacca gatcaaaagc cattgactca
      241 gcaagggttg accgtataat tcacgcgatt acaccgcatt gcggtatcaa cgcgccctta
      301 gctcagttgg atagagcaac gaccttctaa gtcgtgggcc gcaggttcga atcctgcagg
      361 gcgcgccatt tcttcttcat ttaaccccgt cttatccgtt ccgctttgct cttcaccaca
      421 tcactttttg tcgctgtttg gcgagataat tcaacggtgt caggccgtaa aacgccttaa
      481 aaacagagat aaagtacgac gtgctgctat agccacataa ttgcgccacc tgagtgatat
      541 ttttgttatc catcaataac atctgtacgg cgtaacgcat acgacactct gtgacaatct
      601 ggctatagct gatattttcg ttttttaatt tctttttgag caggctgggg cttaaacata
      661 gcgaactggc gacaattcgc agattccagt aatgctgaat atcgctttga ataatgcggc
      721 agacgctgtc gcggacgctg ctgcgtaaga tatacatcag tagggcaata aaccgcgatt
      781 gctcaagaaa gttagacaat acggtaaaaa gcaatgcgcg cgtcaactcc gcttcgcagg
      841 gtttgctggg ttgctgcttg ctgtggttgg cggctagccg gaacacttcc ggtgtacggc
      901 tatgacaagc gataaccggg gttgccagcc gcgaccaggg ctttacacag gttaagttct
      961 tatttaaaaa caacagataa tcgttgatga tgttacggtt gatgtgggtg attaatacgt
     1021 gatttaacgt tgagagttca atgacgttat tgttgcacgc gaaggcggcc aggtagtttt
     1081 tcggaagggt aaacgtagca tggttattga cgcttacctc tacctctttt tcggtcagga
     1141 tcaccacgca aggttcactg ctgctcaatt gcatttcgca ctcctcagat atcagaaact
     1201 ccgctcaaag gatctatgct tcctgcatga gtgatcggcc cgttcgccga taacgatctt
     1261 ctttctttag cacgcttttt agcaattaat cttgatggaa ttctgacgag agcgaaagag
     1321 gtaagccagg tcgtacccga cttacctgga ggagatttaa tactcgagaa tgccgtgcgc
     1381 gacgggcaaa tagcgcagag agaaatagag agtgtcttta ccgcaagagg aacgtttcgc
     1441 taacagcgag gcgatctctg catccggttt cggtttcgct gtaaaggttt tgcctttgcc
     1501 ggcgacaaac agttcataaa caatgccgct tgctggggcg gagctttggc aatcggcgct
     1561 ggcctgtaga taacgttcac gctgttcagc ggtaagttta tccacgccgc tgccatcctg
     1621 tacccagaca ttaactccgg ttgctttaac ttgttccagc agttggcgat agccatctgg
     1681 cgacatgttt ccggcgaaaa aactactgat ataaaccggt tttgctgaaa catcgctaat
     1741 cagccgctgc gcgttgttta accatgttag caaaggctga cgggcagctt cgctgcgcca
     1801 gttcaggtcg tcaatttccg cgctgatgta ccagccatcc ggcgttatgc caggcgcggc
     1861 gctccataat ctggcttgct ggagatcggc agccagcagg cgattaagat agctttccag
     1921 cgctgcggac gactgtttct ggtgcataaa aaattccgga tcggcgttca gcccgacaat
     1981 cagtttcagg ccagcctgtt gcgcagctgc ggcacgctta aacaataacg tgcgctgttc
     2041 tggctgggta aatgcatcgc cgtaacgggt ccattgcaaa accagggtat cgaagccttg
     2101 caaacgtaac tgactcatca gcccctgcca ctgggtatcg gtaacctgac tatctcggtt
     2161 ttgtggttgc cagataatac ctttcatcgc aaaggaaaaa gggctgacca aaagcagtgt
     2221 cagcaatacg aaaatgaact tacgcattta ccagtgcact ccaatggtga gaaacgcgtt
     2281 gttgcgctct ccgttacgtt gattaatcgc cttaaaggta tgttgatact cgacgccgag
     2341 actgactttg tgcggccagg cgtcgtagtg cgtctcgccg gtccagatat tccagcggac
     2401 cccgactccg ccaagctgcg cgccctgagt gcctttatca cgatagccgt tgtcctgaac
     2461 gtgagcgtaa ggctcaatag tctgtccgtt agctaccttc tgatgccagc tgacgcgata
     2521 atctgccgtc cacgcctgaa tatcctggcg gatatattgc gccgcatcga ggtacaggtt
     2581 ttgggcaaac cagcctgaac cgttcgggtg ccattcgtcg ctgtatttgc cgccattaaa
     2641 gaatgaggcg ctggcgcgca gcatggtatc ggatgcgcca ttttgcccgt tcagcggcaa
     2701 ctgctgttcg acggcgagga aaaagatctg atcgcgcagc ggcttccagc gcagaccggt
     2761 gccggacatc ggatttttca ccggcatcat caccccgttt tctccggtat cggcaaagac
     2821 gcggctataa actgagagca ggtcgccttc cagcagcata ttgcgtccga tgcggtactc
     2881 ggcttccagt tgcccgtagc tacgatagct tttccctggc gctgcgccgc caacattatt
     2941 gttagcggta ctcattgccc cggaacgcaa gccgatagaa gaatcgaaac tgaacgtcca
     3001 gcggcgaccg acctcctcat gcaaacggcg gaaattgaag cgttgttgat tttgctctgg
     3061 ggtcagtggg gttatcagag cctgattatc aatgtcatca atcaccagcc gggcgtagtg
     3121 ctgcgtcgca ggcatgtcat ccagacgctg gttcacgtag gccagttgtc ggatcagtgc
     3181 cggatcgtcc ggaagccttt tatgcgcctg ttcgagcatt tcccgcgact gtgcgatatc
     3241 accgctatcc cacaaggcgt aaccaagcgc tgcctggatg ttgctattat tcggttccag
     3301 ttccagcgcg gcgcgcaaat cactcaccgc ggccgggaca ttatgacgtt ggcgataaat
     3361 tgtcgcccgc gcaacgtaag cgttggcaga aggcgcaata ttgattgagc gcgtgagatc
     3421 gttcagtgcg agttccggct gaccaggaat gtaacgttgt gcatgcagcc accagtagag
     3481 ggcattgctt cccagtccac gttgttctgc ctgttgtagc cagcgatcgc gagccgcacc
     3541 atttcctgcc gcctgggcgg tattggcagc agcaagcaga tcctcattgc tcatgtcgtg
     3601 aagactgatt ttctgccagg ccgccagtgc ggtggcgtag tcctcaacct gatacgcctg
     3661 ataggctacc gcacgatgtt gccaggcgct cggttgtcgt tgttcggcct gaagccatgc
     3721 atacaacgcc acaccgggta gcgtgtcccg ataacacttt gccagacggt tccaggcggc
     3781 agcatcgtag gaaggcgaca tatcgcccag caagcgaact attgccgggc aattatctgc
     3841 aatacccggc aactgacttt gccactgacg ttgctccgcc agcggtaagg gtttcgataa
     3901 aatcgccacc ttcgccggcg ttgccaggta aggatgactt tccagcagag acgccagtcg
     3961 cgccattaaa gtctggctga cacgcgcatc gccctggaaa ggatagcgtt gcagcaataa
     4021 atcggcagct tcgcgtgact gctcgttctg catcagttgc caggtcagtt gatccaggcg
     4081 ggtaagattt gccggttctt gctgatacag caatcgtgcc agacgcagag attcagcctt
     4141 gttacgggtc gccacgctga cagcataacg ctcctcaagc atttcattgg cggggagggt
     4201 ggcgagcagt ttttgcgctg cgtcgtactg accttctttt aacagcaccg gtagcgtcgc
     4261 gccaacaaca tactggcggt tatcggcaaa ctgcaccgta taattcgcca atgcctgaac
     4321 ggggttggcg ctgtatttag ataacagata gagccaactt ttctcttgtg cgtccgtggt
     4381 aaacagcggc ttattttcat tgagataatg ctggaggcgt gctttttcgc cacgataagc
     4441 cagcgcggtc gcgtaagtaa tatatgactg aggatcggtg aagatcccct gtgattgcag
     4501 tgctaggatc cgatcgtcca gctgcccggc aagaagcacg tcaaaccact gacggcgttc
     4561 tgccgcgctt aatgtgttct gctggcgtgc ttcattgtat agcgtatctg cctgggacca
     4621 ttgtttcagg tagattgccc gttgcagcag atcggttcgc agcgtttttc cttccggcga
     4681 tgcagcaaac gtcgcatcgt tcagttgcgc tctggcgaca ggtaactgtg caagccgcag
     4741 ggcattctgc ccgacttcac tgcgacaacg cagggtcggc gcagcatcgc acgctttttg
     4801 ctgggcaagc agttcttcaa cagttgtaac gcttttcact tcaaccggaa tagccgccag
     4861 actgcgctca agtcgggcat ctcctgggtg acgtttcagt tgatcctcaa gcaacagccg
     4921 cgcccggtca tcatgaccaa aatggcgata ggcttccgca aggtataaag tcagcggaat
     4981 attatccggc acctgctggt gtatatattc aaattcgcgg atggcggttg cttcgtcgtt
     5041 atttttctgt gccttcagcg ccttatcgag acggggataa ataacaaaat ggcgataatc
     5101 gctcagcccc agctcttctg cgctggtgcc gatattgtct gcgagtgcgc tggtactcaa
     5161 taaagacgtc agcagtaaac cagaccatcc gatgacgcga ttaaggttat tctccttcat
     5221 tttcggactc cagttgcgca acctgttctg tgtttaaacc tgctttgagt aatagtgatt
     5281 gcatcgaaac ttgtaattcg cgttgaattg tcaggacgcg atccaacgtt tcctggctga
     5341 taacgccttc ggtgaccaaa aacttgccga gcggcagaga actgcgttca tggcgcaata
     5401 acaacacgtt aattgctgaa cgattaatat gaccgagcgt ggtcagtatt tcggcgaaca
     5461 ggaactgatg cggcacatat tgccgccaga tttcaccggc ctgctgttcc gtgagccact
     5521 gatgctgaac cgcattgtac aacattgccc gcggatcgtg accgcgtcgg cgtgcatacc
     5581 agtgacgtaa ccccgtgaca atttgtcccc gcagaacaat gaagtaacgc actttgcgtc
     5641 cgactttacg cgtcagggcc gccagcgaaa ccgggtcaat accatcttca ctgccgacaa
     5701 ttaactcatc attttccaga cgcagcggca gtaccgcata atgcagcgcc acggaggccg
     5761 gcatttcggc aatcagcgag gaagggatct gccaggcatc gatggattcc cacgccacgc
     5821 cgttttgctc tgccagcgcc tgtgccagct gctcggcgct aatcagcccc tgcatcagca
     5881 ttgaaccgcc caagcgtaga ccttcgacgc gattacgcag tgctgtatcg agtt
//
LOCUS       DAJTZL010000210         5855 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.211, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000210 DAJTZL010000000
VERSION     DAJTZL010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5855
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.211"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..1687
                     /gene="fecA"
                     /locus_tag="OO544_003706"
     CDS             <1..1687
                     /gene="fecA"
                     /locus_tag="OO544_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Fe(3+) dicitrate transport protein FecA"
                     /protein_id="HCR6769208.1"
                     /translation="LAPVSLGNMNAIDVVRGGGAVRYGPQSVGGVVNFVTRAIPQDFG
                     IEAGVEGQLSPTSSQNNPKETHNLMVGGTADNGFGTALLYSGTRGSDWREHSATRIDD
                     LMLKSKYAPDEVHTFNSLLQYYDGEADMPGGLSRADYDADRWQSTRPYDRFWGRRKLA
                     SLGYQFQPDSQHKFNIQGFYTQTLRSGYLEQGKRITLSPRNYWVRGIEPRYSQIFMIG
                     PSAHEVGVGYRYVNESTHEMRYYTATSSGQLPSGSSPYDRDTRSGTEAHAWYLDDKID
                     IGNWTITPGMRFEHIESYQNNAITGTHEEVSYNAPLPALNVLYHLTDSWNLYANTEGS
                     FGTVQYSQIGKAVQSGNVEPEKARTWELGTRYDDGALTAEMGLFLINFNNQYDSNQTN
                     DTVTARGKTRHTGLETQARYDLGTLTPTLDNVSIYASYAYVNAEIREKGDTYGNLVPF
                     SPKHKGTLGVDYKPGNWMFNLNSDFQSSQFADNANTVKESADGSTGRIPGFMLWGARV
                     VYDFGPQMADLNLAFGVKNIFDQDYFIRSYDDNNKGIYAGQPRTLYMQGALKF"
     gene            1732..2634
                     /gene="fecB"
                     /locus_tag="OO544_003707"
     CDS             1732..2634
                     /gene="fecB"
                     /locus_tag="OO544_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter
                     substrate-binding protein FecB"
                     /protein_id="HCR6769209.1"
                     /translation="MLAFIRFLFAGLLLVISHAFAATVQDEHGTFTLEKTPQRIVVLE
                     LSFADALAAVDVSPIGIADDNDAKRILPEVRAHLKPWQSVGTRAQPSLEAIAALKPDL
                     IIADSSRHAGVYIALQQIASVLLLKSRNETYAENLQSAAIIGEMVGKKREMQARLEQH
                     KERMAQWASQLPKGTRVAFGTSREQQFNLHTQETWTGSVLASLGLNVPAAMAGASMPS
                     IGLEQLLAVNPAWLLVAHYREESIVKRWQQDPLWQMLTAAQKQQVASVDSNTWARMRG
                     IFAAERIAADTVKIFHHQPLTVVK"
     gene            2631..3629
                     /gene="fecC"
                     /locus_tag="OO544_003708"
     CDS             2631..3629
                     /gene="fecC"
                     /locus_tag="OO544_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dicitrate ABC transporter permease FecC"
                     /protein_id="HCR6769210.1"
                     /translation="MTAIKHPVLLWGLPVAALIIIFWLSLFCYSAIPVSGADATRALL
                     PGHTPTLPEALVQNLRLPRSLVAVLIGASLALAGTLLQTLTHNPMASPSLLGINSGAA
                     LAMALTSALSPTPIAGYSLSFIAACGGGVSWLLVMTAGGGFRHTHDRNKLILAGIALS
                     AFCMGLTRITLLLAEDHAYGIFYWLAGGVSHARWQDVWQLLPVVVTAVPVVLLLANQL
                     NLLNLSDSTAHTLGVNLTRLCLVINMLVLLLVGACVSVAGPVAFIGLLVPHLARFWAG
                     FDQRNVLPVSMLLGATLMLLADVLARALAFPGDLPAGAVLALIGSPCFVWLVRRRG"
     gene            3626..4582
                     /gene="fecD"
                     /locus_tag="OO544_003709"
     CDS             3626..4582
                     /gene="fecD"
                     /locus_tag="OO544_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter permease
                     subunit FecD"
                     /protein_id="HCR6769211.1"
                     /translation="MKIALVIFITLALAGCALLSLHMGVIPVPWRALLTDWQAGREHY
                     YVLMEYRLPRLLLALFVGAALAVAGVLIQGIVRNPLASPDILGVNHAASLASVGALLL
                     MPSLPVMVLPLLAFAGGMAGLILLKMLAKTHQPMKLALTGVALSACWASLTDYLMLSR
                     PQDVNNALLWLTGSLWGRDWSFVKIAIPLMILFLPLSLSFCRDLDLLALGDARATTLG
                     VSVPYTRFWALLLAVAMTSTGVAACGPISFIGLVVPHMMRSITGGRHRRLLPVSALTG
                     ALLLVVADLLARIIHPPLELPVGVLTAIIGAPWFVWLLVRMR"
     gene            4583..5350
                     /gene="fecE"
                     /locus_tag="OO544_003710"
     CDS             4583..5350
                     /gene="fecE"
                     /locus_tag="OO544_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter ATP-binding
                     protein FecE"
                     /protein_id="HCR6769212.1"
                     /translation="MTLRTENLTVNYGTDKVLNDVSLSLPTGKITALIGPNGCGKSTL
                     LNCFSRLLMPQSGTVFLGDNPINMLSSRQLARRLSLLPQHHLTPEGITVQELVSYGRN
                     PWLSLWGRLSAEDNARVNVAMNQTRINHLAVRRLTELSGGQRQRAFLAMVLAQNTPVV
                     LLDEPTTYLDINHQVDLMRLMGELRTQGKTVVAVLHDLNQASRYCDQLVVMANGHVMA
                     QGTPEEVMTPGLLRTVFSVEAEIHPEPVSGRPMCLMR"
BASE COUNT         1238 a         1667 c         1665 g         1285 t
ORIGIN      
        1 actggctccc gtttcgctcg gcaacatgaa tgccattgac gtggtacgcg gtggtggtgc
       61 ggtgcgttac ggaccgcaga gcgtgggcgg cgtggtgaac tttgttaccc gtgccattcc
      121 gcaggacttt ggtatcgagg cgggcgtgga aggtcagctc agcccaacct cttcacaaaa
      181 caacccgaaa gagacgcaca acctgatggt gggcggcaca gcggacaacg gttttggcac
      241 cgcgctgctc tactccggca cgcgcggcag tgactggcgc gagcacagcg ccacccgcat
      301 cgacgacctg atgctgaaaa gcaaatatgc gccggatgag gtgcacacct tcaacagcct
      361 gctgcaatat tacgacggtg aagccgacat gcccggtggc ctgtctcgcg cggattacga
      421 cgccgatcgc tggcaatcca cccgcccgta tgaccgcttc tggggtcgtc gcaagctggc
      481 gagcctgggc taccagttcc agccagacag ccagcataaa ttcaacattc aggggttcta
      541 cacccaaacc ctgcgcagcg gctacctgga gcaaggcaaa cgcatcaccc tctcgccgcg
      601 taactactgg gtgcgcggta ttgagccacg ctacagccag atctttatga tcggcccttc
      661 cgcgcacgaa gtgggcgtgg gctatcgcta tgtgaatgaa tcaacgcatg aaatgcgtta
      721 ctacaccgcc accagcagcg ggcagttgcc gtccggctca agcccttacg accgcgatac
      781 gcgttccggc accgaggcgc acgcctggta tctggatgac aaaatcgaca tcggcaactg
      841 gaccatcacg ccgggtatgc gtttcgaaca tatcgagtca taccagaaca acgccatcac
      901 aggcacgcac gaagaagtga gctataacgc accgcttccg gcgttgaacg tgctctatca
      961 cctgactgac agctggaatc tttatgcaaa cactgaaggc tcgttcggca ccgtacagta
     1021 cagccagatt ggcaaggctg tgcaaagcgg caatgtggaa ccggaaaaag cgcgaacctg
     1081 ggaactcggt acccgctacg acgacggcgc gctgacggcg gaaatggggc tgttcctgat
     1141 taactttaac aatcagtacg actccaacca gaccaacgac accgtcactg cacgtggcaa
     1201 aacgcgccat accgggctgg aaacgcaggc acgttacgat ctgggtacgc taacgccaac
     1261 gcttgataac gtttccatct acgccagcta tgcgtatgtg aacgcggaaa tccgcgagaa
     1321 aggcgacacc tacggcaatc tggtaccatt ctccccgaaa cataaaggca cgctgggcgt
     1381 ggactacaag ccaggaaact ggatgttcaa tctgaacagc gatttccagt ccagccagtt
     1441 tgcggataac gccaatacgg tgaaagagag cgccgacggc agtaccggcc gcattcccgg
     1501 cttcatgctc tggggcgcac gcgtggtgta tgactttggc ccgcagatgg cagatctgaa
     1561 cctggcattc ggtgtgaaaa acatcttcga ccaggactac ttcatccgct cttatgacga
     1621 caacaacaaa ggcatctatg caggccagcc gcgcacgctg tatatgcagg gagcgttgaa
     1681 gttctgatgt gttaacgccc ggcttgccgg gcttttagct ggaatgtgat tatgttggca
     1741 tttatccgtt ttctttttgc aggcctgctg ctggtgatca gccacgcctt tgccgccacg
     1801 gttcaggacg aacacggcac gtttacactc gaaaaaacgc cacaacggat tgtggtgctg
     1861 gaactctcgt tcgccgatgc gctggccgcc gtggacgtca gcccgatcgg tattgccgac
     1921 gataacgatg caaaacgcat cctgcccgaa gtgcgtgcgc acctgaaacc gtggcagtcc
     1981 gtcggaacgc gcgcgcagcc gagcctggaa gccattgccg ctctgaaacc agacctgatc
     2041 attgccgaca gcagtcgcca tgcgggggtt tacatcgcct tgcagcaaat cgcgtcggta
     2101 ctgctgctta agtcccgcaa cgaaacctac gctgaaaatt tgcaatctgc ggctatcatc
     2161 ggcgaaatgg tgggtaaaaa gcgagagatg caggcacgtc tggaacaaca taaagagagg
     2221 atggcgcagt gggccagcca gcttcccaaa gggacacgcg tggcctttgg cacatcacgc
     2281 gaacagcaat tcaacctgca tactcaggag acctggaccg gcagcgtgct ggcctctctg
     2341 gggctgaacg ttcccgctgc gatggcgggt gcgtccatgc cgtccatcgg cctggagcaa
     2401 ctgctggcgg tcaatcctgc ctggctgctg gttgcccact atcgcgaaga gagcattgtt
     2461 aaacgctggc aacaagatcc gctctggcag atgttaaccg ccgcgcagaa gcagcaggtt
     2521 gcttcggtcg acagtaacac ctgggcgcgg atgcgcggta tttttgctgc agagcgtatt
     2581 gccgctgaca cggtaaaaat cttccaccat cagccgctta ccgttgtgaa atgaccgcga
     2641 taaaacaccc ggtgctgctg tgggggcttc ccgttgcagc acttattatt attttctggc
     2701 tgagtctgtt ttgctactcg gccattcctg tttccggagc agatgcaacc cgcgccctgc
     2761 tgcctggaca cacgccaacg ctaccagaag cgctggtgca aaaccttcgt ttgccacgaa
     2821 gcctggtcgc cgttctgatc ggcgcaagcc tggcgctcgc gggcacgctg ctgcaaaccc
     2881 tgacccacaa cccaatggcc tctccttcac tgctcggcat taacagcggc gcggcgctgg
     2941 ctatggcgct taccagcgcg ctgagtccga cgccgattgc aggctattct ctgtcgttca
     3001 tcgcggcatg cgggggcggc gtgagctggc tgctggtcat gaccgcagga ggcggatttc
     3061 gtcataccca tgacagaaac aaactgatcc tcgcgggtat cgcgctgtcg gccttttgta
     3121 tgggccttac ccgcatcacc ctgctgctgg ccgaagatca tgcttacggc atcttttact
     3181 ggctggctgg cggagtgtcc cacgcccgct ggcaggatgt ctggcagctc ttgccggtgg
     3241 tggtcactgc agtccctgtc gtgttgctgc tggcgaatca actgaacctg ctcaacctca
     3301 gcgacagcac cgcccatacg ctgggagtga acctgacgag gctatgtttg gtcatcaata
     3361 tgttagtgct gcttctggtt ggtgcgtgcg tcagcgtggc aggtccggtg gcgtttatcg
     3421 gtctgctggt gccacatctg gcgcgcttct gggcaggctt cgatcagcgc aacgtactgc
     3481 cagtgagcat gctgctgggg gccacgctga tgctgctggc agatgtactc gcacgcgcgc
     3541 tggccttccc cggagatctg cccgcaggcg cagtgctggc gctgattggc agcccttgct
     3601 ttgtctggct tgtgaggagg cgaggatgaa aattgcgctg gttattttca tcacccttgc
     3661 cctggcgggc tgtgcgctgt tatcactcca tatgggagtg atccccgtgc cgtggcgcgc
     3721 gctgctgacc gactggcagg ccggacgcga gcattattat gtattgatgg agtaccgact
     3781 gccgcgcttg ctgctggcac tgtttgtcgg tgcagccctc gccgtggcgg gcgtgctgat
     3841 acaggggatt gtgcgcaacc ctctggcatc accggatatt ctcggcgtta accatgccgc
     3901 cagcctggcc tctgtggggg ctctacttct tatgccgtca ctgcccgtga tggtgctgcc
     3961 gctgctggcc tttgcgggcg gcatggcggg gttgatattg ctgaagatgc tggcaaagac
     4021 ccaccagccg atgaagctgg cactcaccgg cgtggcgctt tctgcatgct gggccagcct
     4081 gacggattat ctgatgctct cgcgcccgca ggatgtgaac aacgccctgc tgtggctgac
     4141 cggcagctta tggggccgtg actggagctt tgtgaagatt gccatcccgc tgatgatttt
     4201 atttctgccg ctgagcctga gtttttgccg cgatctcgac ctccttgcac tcggcgatgc
     4261 gcgcgccacc acgctcggtg tgtcggtgcc ctatacccga ttctgggctt tgttactagc
     4321 tgtcgccatg acatctaccg gcgtggccgc ctgcggcccg attagcttta ttggtctcgt
     4381 ggtgccgcat atgatgcgta gcatcaccgg tggacgtcac cgcagactgc tgcctgtttc
     4441 ggccctgaca ggtgcgttgc tgttggtggt tgccgatctg ctggcgagaa ttattcatcc
     4501 cccactggag ctcccggttg gcgtgctgac cgccattatc ggtgcgccgt ggtttgtctg
     4561 gttgcttgtg agaatgcgat aaatgacttt acgaactgaa aatctgacgg tcaattacgg
     4621 aacagacaag gtacttaacg acgtttcact ctcactgcca acggggaaga tcaccgccct
     4681 gatcggtcct aacggttgcg ggaaatcgac gctgttaaac tgtttttcgc ggcttttaat
     4741 gccgcagtct ggcaccgtat ttctcggcga taatcccata aatatgctct catcgcgcca
     4801 gttggcccgc aggctttcgc tgctgcctca gcaccattta acgccagagg ggatcacagt
     4861 ccaggagctg gtttcgtatg gtcgtaatcc ctggctgtca ctctgggggc gtctctccgc
     4921 tgaagacaat gcacgagtta atgtcgccat gaaccagacc cggatcaatc atcttgccgt
     4981 tcgtcggtta accgagcttt ccggcggtca gcgccagcgc gcatttctgg caatggtcct
     5041 ggcccagaat acgcccgttg tattacttga tgagccaacc acctatcttg atatcaatca
     5101 ccaggtggac ctgatgcggt tgatgggcga actccggact caggggaaaa cggtggtcgc
     5161 tgtgctgcac gaccttaatc aggctagccg gtactgcgat caactggtgg taatggcaaa
     5221 cggacatgtt atggcgcaag gcacaccaga agaggtgatg accccaggat tgctgagaac
     5281 agtattcagc gtggaagcgg aaatacaccc cgagccggta tctggcaggc cgatgtgcct
     5341 aatgaggtag attgcacagg ccgtaagaac caaaccacga ctgaatgaaa ctggactggc
     5401 gccagcaagc ctgttcagac tggggctgaa cttttccgga ctctgaaaga ttaccaatac
     5461 tcatcgtcca tccgcttgct ttaggctgac aggttcataa tcaacgcaaa ccagagctgt
     5521 acaggcttgg gcgcggcttt caaaccagtc gtgatcacgg caatcaattt tgaactctgc
     5581 ttaacggaca tttctgtata acccttacgg caacgaaaaa cgcgaagtta aaattttaga
     5641 aacccaaaaa cgtgacatga ctaagtttag atttcagggg ggggagatca aaaaatttcg
     5701 ctctgtgcca gagcggacat tcacggagct ggttcattac caatgaggtt gggcttttga
     5761 ggataaatca atgatcagac gccaacgtaa atcaaaagca accctggaac ggaattgcta
     5821 atccagtttc tgaccatcga tttttctaaa aagtg
//
LOCUS       DAJTZL010000211         5836 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.212, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000211 DAJTZL010000000
VERSION     DAJTZL010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5836
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.212"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..242)
                     /locus_tag="OO544_003711"
     CDS             complement(<1..242)
                     /locus_tag="OO544_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR6769213.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSI"
     gene            complement(604..3387)
                     /locus_tag="OO544_003712"
     CDS             complement(604..3387)
                     /locus_tag="OO544_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCR6769214.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNGLRYMI
                     YPHAQPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETLYQVSLPTTQKQNLQQVMAIFSEWSNAATFEILEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFVEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNELAAKKALSLAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGARVILAKSAGEEQKLQITAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYETRYADDRTKLLQENQIVQFTA
                     ADALAADRQLFSSPADITFVIVGNVSEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLAKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     HPVNTYTGVLLPK"
     gene            complement(3444..5816)
                     /locus_tag="OO544_003713"
     CDS             complement(3444..5816)
                     /locus_tag="OO544_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HCR6769215.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRSDWLTSHIDENNKSAFNQGSSGSTYYSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVTGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTCGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMAEHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQDLKTPYNDELAMGLQQKIGKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFSLSFELAEPLHIRQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
BASE COUNT         1487 a         1320 c         1312 g         1717 t
ORIGIN      
        1 ttaatggata aatccatcaa tttgtggaca ttttcgtcgt cccaggtctg gcagaaagtg
       61 gccaggttct gacgagcgtt gccatcaaga tataattcgt cattgataat ctggaatgcg
      121 acatcgtcgc gcatttcgtg cagcggaaaa cgttttgatt ctgcgatagt ggaaatagac
      181 ttcgcaccaa aacgtgaatc gagtagttcc gaccttaaat ccgttacttg cttcttatcc
      241 attttaaact ccttaaaatg attggatagc attaaaaaag taggatttat cgataaagta
      301 agcaagtgca aaggactcgt gtttaaataa caaaatccta atgttattta tcgtgagata
      361 ttgcgagaat aatatttttt cattgaaaaa caatataata tgaaattctt gggtggtggt
      421 aaggtgtttt atgctgttat ttttatgcgc attctgtgcc tcctgaattt tcacgtaaaa
      481 atcagacctt aaaatatcac tattagtacc tggttattat tttgaacgca tttataaaat
      541 tattacataa aaatggcgaa tattgctaaa atccccgcca acgatgtgtt gacggggctg
      601 ttattatttt ggcaataata ctccagtata agtatttacc ggatgagaaa gatattgttt
      661 aacggcagtg ttaacattct ctaccgtcat ttgtttcaac aattgctcct gctcagtcca
      721 tgctgcagga tcgtcatatt gaataagact atttacaata gtgttcgcta attgttgaac
      781 gctacgctgt tggatatcga ggctgcgctg aacgttttgc tggtattcat tcagttcttg
      841 ctcactgatc cctttagcca gacgcttaac catcacttca ttcgctaacg ttaacagttc
      901 atcatgtcgt tctggttgac aagtaaaagc cagcaaatga ctgatatctt tggcctgagg
      961 atcaaccgag aggcgagaag aaacgctgta tgctccagat gcctgttcac gaatattaac
     1021 gcgtagatct tttgccagtg cgacgttaaa agcatcgagc gccatacgcg tcgccagatt
     1081 aacaggtgtc cgggaatcat aacgcttcca ctgtgaaacc tgtgccacag gttcattttg
     1141 ttcttttaca gtaaccgatg cgttgtccgt cgcgcgagtt aatggtttac ctgcggctaa
     1201 tggcgaatca gagtgtttga ttgatcctaa gtaacgcgta attaacgcca cgagtttgtc
     1261 ttctgagaca ttaccgacaa tgacaaacgt gatatccgct ggagatgaaa acaactggcg
     1321 atcggcagcc agcgcatctg cggcagtaaa ctgtacaatc tgattttctt gcagtaattt
     1381 cgtgcggtca tcagcatagc gcgtctcata catctgctgg gcgaatttct ccgctggacg
     1441 ctggtcgagc gttttcaacg cctgaatttg agcattttgt agcgatgccc aaatattgtc
     1501 gttaatcgtg ctgtgggtga ttcgctggtt aatcaactga aaaccaggtt cagggttatt
     1561 agtccgcgcg ctaacggaga gcaacgtatt catgccactg actttactgc tcatggttac
     1621 cgaattttcc gcactccagc gtttcaggct ggaagaggag agctcaccaa cgccgcttcc
     1681 gcttactgct ttatttgcca gcgcgataag tgatttttgc tgcgcaggga aacttaaatc
     1741 gcctttatta gatacggcag taatttgtag cttttgctct tcacccgcgg attttgccag
     1801 aataaccctg gcaccattgg aaagtgttaa tgatgtcaga ttctcagcca gcgtttcttt
     1861 gctgctaatt tccgcctgtg ggtcagcgtc tactgttaac gataaatttc tgcctgggaa
     1921 gatgtaagcc gccagctttt tgttggcgta ctctttttcc agcgccagga tagccgcaag
     1981 agacaatgct tttttggcgg caagctcatt gtttaccatt tgctcccaaa atgcgtcctg
     2041 gttctttctt aactgctgcc atttttccgc cagactttgc acggtaattt gctgccacag
     2101 acgtttcgaa agttgatatg tctcttccgg cgacaagaac ggcgtattat ttaatgagct
     2161 ggatgccagg cgactggtca gcatacgtaa atcacgttca gcttgctgat caaccgcatt
     2221 tttcagccag gtgaggcggg tagatttgac atcatcgagt tcttcagcag aaaaaccatg
     2281 ctgatcaatg gttgccaact ctgccattaa tgcatttgca gcatcctgca tattatcgtc
     2341 tcgtgcattt acacggaaaa acagcgactg ataatcgggt gcaattttaa cgctgcgcgc
     2401 agtgccgcca gaaatagtct tcaactcgcc cgactgtatg cgttcctgca gacgttgatt
     2461 gaacagctga actaacatgc tccattcagc ttgttcgaca aagctttgct catcgttcac
     2521 ttgtaccatt ggcaggcgat aatagagtgc gatgccgttc actcggtttt ctttatcatt
     2581 aatgatatta aagcgcaggt ggttttcggc ttttgtcggc cagacgcgat tttcagctgc
     2641 tttgttagcc ggaagcttac ttaaattatc ctttatcagc gccagcgctt ctttactgtc
     2701 gatatcgccg accacgataa aggtcatatt atttggttga taccagcgtt gataaaattg
     2761 gcgcaattgt gccggtgtga ccgtggcgac agtatccatc aggccgatag gttcacggtc
     2821 taaattacgg gtatttgcca gcaggaaagg gcggcgcgcc tgagaggtgc gccatttcgc
     2881 atcctgatgg gcacgccatt cctcagtaat tacgccgcgt tcagcgtcta cttcgagtat
     2941 ttcaaaggtt gcggcattac tccattcact gaagattgcc atcacttgtt gcagattttg
     3001 tttctgcgta gtcggcagac tcacctgata cagcgtttcg tcatagctgg tataggcatt
     3061 aacatcgcga ccgaaacgca ggcccattga ctcaaatgtt tcgatgactt tattacccgg
     3121 ccatgttttt gtgccgttaa acatcatatg ttctacaaaa tgagccacgc cgcgctcatt
     3181 gtcttcttcc tgcaatgaac cggtatgaat ttgcagccat aaatttacct gatcctttgg
     3241 ttgagcatgc ggataaatca tatatcgcaa gccattgtcc agttgcccgg taattaactt
     3301 ttcatcctgc ggtaaggcgg cggcaatcag ccgcccgggg agcaacagag ttgccactaa
     3361 cgtcagtaag aaacagaggt ttctcataat tatctccatg cgaaaaccgg gcgaatttac
     3421 ccggttaagt aaaatccgaa ctattaaaat ttcatgctga catcaagcca gaaagtacgg
     3481 ccactggcgt aggtcgccac accggtgttc gttgtatcaa cagcggtttt gctatccagt
     3541 acattgagaa tatcggcact gatcgtcagg ttttgttgtt tcagaaattg tggcgtccag
     3601 gacaagcggg tgtcccaggt caggctgcta tccagttttt cgtcaacata ctgcttgtaa
     3661 tctgaatatt cgctgatgta ttgcgcattt gtcttaccaa ggataatgcg agctttacgc
     3721 gcttcttgcc aggccagcgt attcgcccac actaacccgc tcggttgatg cgtgaaatcc
     3781 atgtttaagg agatctttaa tgggttatta aaatctgcca ctggaacact atcgtaagag
     3841 accagattac cgttataaac cacctggtta tcaccggtat tgctctcctc ataaccattg
     3901 tttaacgaca agttgccctt gctcttgata tagctaaaga caatttgtgg gttaatatct
     3961 acttggcgga tatgcagggg ttcggccagt tcaaaactga ggctgaacga atgcgttttg
     4021 gttttgccat cgttgttata ttcagtaatg gtggttttag tcgcgctgtc ggtacgactg
     4081 cttttgctga tttgatcatg cgcttcacgg taaacatagt ttgcacgcgc aataacgttc
     4141 ttaccgattt tctgctgcaa tcccattgcc agttcatcgt tataaggcgt tttcaaatcc
     4201 tgataacgcg tcagagtttt attacctgat accgattccg tccagctatt gcggatatca
     4261 cgtaatccca tatcaagaat attcccgcca tagtaacggt tataaccggc ggtaatcatt
     4321 gaggtttgat cagcaaaaat atcccattcc gtcataaagc gcggggagat attgtggttt
     4381 gacagatagt tgtcatagtc ataacgcaca ccgggcatta acgacacatt ccgccagcta
     4441 atgtgctccg ccatatacag cgtgtagttg tcaattccca ggctgccttt acctttatgg
     4501 taaatggtat ggttagtttt ctttccggca gcattaatca cataggattc agactggtta
     4561 tggcgttcag tccatgcatc ggaatagatg tattccgcgc cgaagtaggg ttgatgcgaa
     4621 acatcaccca cggcgaattt ttgccagtcc aggcgtgttt tgaaggtgta attatctaca
     4681 gcctgggaaa tgtgtcctaa tccgccacag gtacaacgcc ctgtaatatc accatatgta
     4741 catgaaagtt cggtgtacca gatgtcatga tcgtgacggg tataatcact aatatgatcc
     4801 caaccaacgg tggtacgtag tttggcccat gcgagttgcg tatccatatc ccatgccaga
     4861 ccataggatt tattacccat ttcgcgatca gactgcggga aggtgctggt attataatca
     4921 cggctggagc cggtatattt taaggttaaa tcgtgggtaa agcggtcgct ggcaaaccag
     4981 gtaaatttgc tcaatgcagt atcgataacg tttttatact gtgcccgacc ggtgacaatg
     5041 ccgtcattcg aaacataatc cgcgcgggtg atatcagact ggcggcgcga taaaccggcg
     5101 gtaacgccga agttatcagc gagttcctga ttaaacgaca aggtataaaa gttcttttta
     5161 aaatctgggg agtaataagt acttcctgaa gagccttgat taaatgcgct cttgttattc
     5221 tcatcgatat gcgatgttaa ccagtccgaa cgcgtagtgc gataacccaa tttcaccttg
     5281 ctatcatcag cgttgaagcg tttgatcttt gcatcaatta ccccgccatt gaagcgacca
     5341 aattcaaccg gcacaaaact gtcataaagc gtcacattgt ccagtaagct gacatcaaga
     5401 taatacccct gtgacatccc gctaatattg gttgcactac tggcatcgga ctcattcgct
     5461 gggttcaggt tattagttgc actaataccg tcaatcaaat aggcattctg gtagggcgac
     5521 gcaccgtgaa tagagatttt ctcagggcga atatctccct ggttcaacga ggtactttgc
     5581 gttgaatcca tgcgtaccgc agggttggtt ctcagcagat cgctgatatt gccattcccg
     5641 gtcggtaaac gctcgatgct ttcactggtg taatgcgtgt tgccgtttac gggtacaggg
     5701 acaggagcat agaccgtcat ctcttcaaaa aaatgcatgt acatgttttt gtcatcgacg
     5761 gcctgcgcca catcagcgcc acataaaatg acgccaggaa taagaactcg cttcatttat
     5821 ttatatctcc gtacag
//
LOCUS       DAJTZL010000212         5614 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.213, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000212 DAJTZL010000000
VERSION     DAJTZL010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5614
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.213"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            12..428
                     /gene="soxR"
                     /locus_tag="OO544_003714"
     CDS             12..428
                     /gene="soxR"
                     /locus_tag="OO544_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001129347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HCR6769216.1"
                     /translation="MAKRSGVAVSALHFYESKGLITSIRNSGNQRRYKRDVLRYVAII
                     KIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREELDRRIHTLVALRDELDG
                     CIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            974..2323
                     /gene="ghxP"
                     /locus_tag="OO544_003715"
                     /gene_synonym="yjcD"
     CDS             974..2323
                     /gene="ghxP"
                     /locus_tag="OO544_003715"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HCR6769217.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            2475..4124
                     /locus_tag="OO544_003716"
     CDS             2475..4124
                     /locus_tag="OO544_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HCR6769218.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(4278..5569)
                     /locus_tag="OO544_003717"
                     /pseudo
     CDS             complement(4278..5569)
                     /locus_tag="OO544_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
BASE COUNT         1256 a         1270 c         1456 g         1632 t
ORIGIN      
        1 cccccggcga agtggcgaaa cgcagcggtg tggcggtatc ggcgctgcat ttctatgaaa
       61 gtaaagggtt gattaccagt atccgtaaca gcggcaatca gcggcgatat aaacgtgatg
      121 tgttgcgata tgttgcaatt atcaaaattg ctcagcgtat tggcattccg ctggcgacca
      181 ttggtgaagc gtttggcgtg ttgcccgaag ggcatacgtt aagtgcgaaa gagtggaaac
      241 agctttcgtc ccaatggcga gaagagttgg atcggcgcat tcatacctta gtggcgctgc
      301 gtgacgaact ggacggatgt attggttgtg gctgcctttc gcgcagtgat tgcccgttgc
      361 gtaacccggg cgaccgctta ggagaagaag gtaccggcgc acgcttgctg gaagatgaac
      421 aaaactaaag cgccacaagg gcgctttagt ttgttttccg gtctttgtct ttctctctat
      481 cccgctggta cacaggaggg tttcccccga cgtcaacaca ccccattcga gcacgtggtg
      541 gaggttccgg ttggtgttga tgctttaatt gtatgtcacc ggcatttctt cgccagtgta
      601 aaagtatact ttttatccgc aatatttttg tcatctcaga cgatttttta tcgcaatcct
      661 gaacggtata cggctcgata acgctgcaat cttgcgcacc gacgataacg tttgcgcatc
      721 aattgcctgg tttttcatcg tcaagacaat aaaagagaaa aaagcagcaa acttcggttg
      781 aaaaagccgc tatgatcgcc ggataatcgt ttgctttttt taccacccgt tttgtatgcg
      841 cggagctaaa cgtttgcttt tttgcgacgc agtaaattgt cgcaaacctg gagcaggaag
      901 ataacgtttc tctggcaggg gattgtccgc cacgcatctt gacgaaaatt aaactctcag
      961 gggatgtttt cttatgtcta cgccatcagc gcgtaccggc ggttcactcg acgcctggtt
     1021 taaaatttca caacgtggaa gcactgtccg tcaggaagtg gttgccgggt taacaacgtt
     1081 tctggcgatg gtctactcgg tcatcgtcgt tccaggtatg ttgggtaaag caggcttccc
     1141 gcctgcggca gttttcgttg caacctgtct ggttgccgga ctcggttcta tcgtgatggg
     1201 tctgtgggct aatctgccgt tggcgattgg ttgcgccatc tccctgacgg cgtttaccgc
     1261 attcagcctg gtgctggggc aacatattag cgtacctgtc gcgctgggtg ccgtgttcct
     1321 gatgggtgtg ctgtttacgg taatttctgc cacgggtatc cgtagctgga ttttgcgcaa
     1381 cttgccgcac ggtgtggcgc acggcacggg gatcggtatc ggtctgttcc tgctgctcat
     1441 tgccgctaat ggtgtcggtc tggtgattaa aaacccgctt gatggtctgc ccgttgcgct
     1501 gggtgatttc gcgaccttcc cggtgattat gtcactggta ggtctggcgg tgatcatcgg
     1561 cctggaaaaa ctgaaagtcc ctggtggcat tctgctgacc attatcggta tctcaattgt
     1621 cggtttgatc ttcgatccta acgtccattt ctccggcgtt ttcgccatgc cttcattgag
     1681 cgatgaaaac ggcaattcac tgattggcag cctggacatt atgggcgcgc tgaatcctgt
     1741 agtcctgcca agcgttctgg cgctggtgat gacggcagta tttgatgcca ccggaactat
     1801 ccgtgccgtc gccggccagg cgaacctgct ggataaagat gggcagatca tcgacggtgg
     1861 gaaagcactg accactgact ccatgagcag cgttttctct ggcctggtgg gtgctgctcc
     1921 ggcagcggta tacatcgagt ctgcggcggg tacggcggcg ggcggtaaaa ccggtttgac
     1981 ggctatcacc gttggcgtgc tgttcctgct gattctgttc ctctctccgc tctcttacct
     2041 cgttccgggg tatgcaacgg ctccggcgct gatgtacgtt ggcctgctga tgctgagcaa
     2101 cgtggcgaaa atcgactttg ctgattttgt tgatgcgatg gcgggtctgg ttacggcggt
     2161 attcatcgtg ctgacctgta acatcgtaac aggcatcatg atcggcttcg cgactctggt
     2221 gattggtcgt ctggtttccg gcgaatggcg caagttgaac atcggtacgg tcgttatcgc
     2281 cgtggcgctg gtgaccttct atgcgggtgg ctgggctatc taatctttca tcggattctg
     2341 aaaacgggtg gcaatggctg cccgttttta ttttctccgc acattgtgtg agcttttgcg
     2401 atattctgaa aaaatgagaa ttcaggcata acgcctttct cagatcataa aaaaacatcg
     2461 caaacaggga acgcatggaa atcttcttca ccatactgat aatgaccctc gtggtctcac
     2521 tgtccggggt ggtcactcgt gtcatgccct ttcagatccc gcttccgctt atgcaaatcg
     2581 ccatcggtgc gctactggcg tggccgacgt ttggtttgca tgtggagttt gatcctgaac
     2641 tctttttagt cttgtttatc ccgccgttgc tgttcgctga tggctggaaa acgccgaccc
     2701 gtgaatttct cgaacatggt agagagattt tcggcctcgc gctggcgctg gtggtggtca
     2761 ccgtggtcgg cattggcttc cttatttact gggtggtgcc gggcattccg ctgatccccg
     2821 cctttgcgct ggcggcggta ctttctccga ccgatgctgt ggcgctctcc gggattgttg
     2881 gcgaagggcg catcccgaaa aaaatcatgg gcattttgca gggcgaagcg ttgatgaatg
     2941 acgcctccgg cctggtgtcg ttgaagtttg ccgtggcagt ggcgatgggg acgatgatct
     3001 tcaccgtcgg cggtgcgacg gtcgaattta tgaaagtagc cattggtggt attcttgccg
     3061 gttttgtggt gagctggctg tatggtcgct cgctgcgatt cctcagccgc tggggcggtg
     3121 atgaacccgc gacgcagatc gttctgctgt tcttgctgcc attcgcttct tatctgattg
     3181 ccgaacatat tggcgtttcc ggcatcctcg ctgcggttgc cgccgggatg accatcaccc
     3241 gctccggtgt gatgcgccgt gcgccgctgg caatgcgcct gcgcgcaaac agcacctggg
     3301 cgatgctgga atttgtcttt aacggcatgg tgttcctgct gttaggtctg cagctgccgg
     3361 gtattctgga gacgtcgctg atggcggcag aaatcgaccc taacgtcgaa atctggatgc
     3421 tgtttaccga tattattctg atatatgcgg cgctgatgct ggtccgtttc ggctggctgt
     3481 ggacgatgaa aaagttcagc aaccgcttcc tgaagaagaa gccgatggag tttggttcgt
     3541 ggaccacacg agaaatcctg atcgcgtctt tcgccggggt gcgtggggcg atcactctgg
     3601 ccggtgtgct ctctatcccg ctgctcttgc cggatggtaa cgtcttcccg gcgcgctatg
     3661 agctggtgtt cctggcggct ggcgtcattc tcttctcgct gtttgtcggc gtggtgatgt
     3721 tgcctattct gctacaacac attgaagtcg cggaccattc gcaacaattg aaagaagaac
     3781 gtattgcgcg agcggcaacg gcagaagtgg cgattgtggc gatccagaaa atggaggagc
     3841 gtctggcggc ggataccgaa gagaatatcg ataaccagct gcttacggag gtcagttctc
     3901 gcgtcattgg taacctgcgt cgtcgcgccg atggacgtaa cgacgttgaa agttccgtgc
     3961 aggaagagaa ccttgagcgt cgcttccgtc tggcggcatt gcgttctgaa cgtgctgaac
     4021 tgtaccacct gcgcgccacg cgggagatca gcaacgaaac gctgcaaaaa ttactgcacg
     4081 atctcgattt gcttgaagcg ttgctaattg aggaaaatca gtaagccgaa agcgacgtaa
     4141 ccaggcccga ggtaatctga aaaaggtgca atcttttgca cctttaacag gttagctcgg
     4201 gctaacattc atgattctaa aacaaataag ttatctctct tgtaaagtca attcattggc
     4261 tgttcttcga cttaacctca atctgcgtgt tgcataatat ctgcagtaat cttcatagcg
     4321 actgcattaa tattatcctc agtttgtgac atgccagtat gagaggtaca atcttcttca
     4381 aagaatttct gtagtgttac cagggtatca ctatcaatgg ggatattttc ctccccacgg
     4441 agtatggaaa tgcccattcc ctggacgcta gcctcatcaa aattaacccg atagagacgg
     4501 gtattaaaca tacaggcttt tgttaaaata gaacagttga aatggcagtt gcttaaattg
     4561 gcattattaa aattaacatt agccatatta gagtcggtac atataaccat ttcgaggaat
     4621 gtatttgtta gccacgtaca aaagagtttg ctatcaatta agtttatatt ctggaggatt
     4681 gctttgttca gattgacgcg agacagatca gcatgttcaa agttaattaa atcgagttta
     4741 gagaaactaa ggtcggcttt aaataaatta acatatggcg ctattacttt ataaagattt
     4801 gcctcagcca tgtacgccat ataaaaattt gaatagtcaa gaaaggtatt tttaatattg
     4861 gctctattca ttgctgcatt gtagaattga acatgcataa cggagcatgc atttagagtt
     4921 ccgtcggcca tttttatctg agcgaaattt gagtcatcga gaatacaatt atcaaaaagt
     4981 atttttccat tatggcgtaa taaaaattcg ttttatacat attacagtcc tggaaaatag
     5041 cgttttgtag tctgcaacct gcaaaattcg aattggataa atcttcatct ttaatcacga
     5101 tacctttcag gctaaggtgt gaaaaatcgt aaccaccatt tactgtctgt ggaatttcga
     5161 tagtattatt atggacggcg acattattta taaaatatac caccttaatg gatacttcct
     5221 tttctttact aaggtaaact cttctcgtaa ttctatcgtc acatttatta tttgcatata
     5281 aagctatatc tataaaatgt ttatgtattt tcctgtccgt ttccttcgtg atttttcctg
     5341 atggtaaact atcataaatt cttttcattt tctgaatcaa acctatcact ttttcatgat
     5401 catccatctt gaaaagagag agggctttat tgtcaattaa cgtgttttta tgatgtttgc
     5461 tgtaattata tgattttgtt ttctttgtat gtgatgaact tgtgaaagag tggttatcgt
     5521 taatctggca aagagagaaa aacatattat ttaaaccatt atagcgcata aaatatcctt
     5581 ataattaaca agaaagaaaa ggcatattct ctgc
//
LOCUS       DAJTZL010000213         5590 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.214, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000213 DAJTZL010000000
VERSION     DAJTZL010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5590
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.214"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(506..1303)
                     /locus_tag="OO544_003718"
     CDS             complement(506..1303)
                     /locus_tag="OO544_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6769219.1"
                     /translation="MLAKDRTNLKIEEIRMHKHHEIHRVKPLMPASCRIRQGKKVINW
                     ETHSLTVDNNQIILFPCGYEFYIANYPEAGLYLAEMLYYPIDLIEKFQNLYAITDQIR
                     NTTGFCLPQNSELIYCWEQLKTSISRGFSTQIQEHLAMGVLLSLGAHHVNCLLLSDSK
                     QSLISRCYNLMLSEPGTKWTANKVARYLYISVSTLHRRLASEGVSFQSILDDVRLNNA
                     LSAIQTTVKPISEIARENGYKCPSRFTERFHNRFKITPRELRKASRE"
     gene            complement(1313..1864)
                     /gene="ybcL"
                     /locus_tag="OO544_003719"
     CDS             complement(1313..1864)
                     /gene="ybcL"
                     /locus_tag="OO544_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HCR6769220.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGV
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPADAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKIWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEIIPVYEVK"
     gene            complement(2033..2365)
                     /gene="emrE"
                     /locus_tag="OO544_003720"
     CDS             complement(2033..2365)
                     /gene="emrE"
                     /locus_tag="OO544_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family multidrug efflux protein EmrE"
                     /protein_id="HCR6769221.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            complement(2699..3013)
                     /gene="fliE"
                     /locus_tag="OO544_003721"
     CDS             complement(2699..3013)
                     /gene="fliE"
                     /locus_tag="OO544_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HCR6769222.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            3229..4887
                     /gene="fliF"
                     /locus_tag="OO544_003722"
     CDS             3229..4887
                     /gene="fliF"
                     /locus_tag="OO544_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
                     /protein_id="HCR6769223.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYCFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     QSNTSGRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESHAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMKQIEDLTREAMGFSEKRGDSLNVVNSPFNSSDESGGELPFWQQQAFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTRRAEAMKAVQQQAQAREEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            4880..>5590
                     /gene="fliG"
                     /locus_tag="OO544_003723"
     CDS             4880..>5590
                     /gene="fliG"
                     /locus_tag="OO544_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HCR6769224.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTYVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFE"
BASE COUNT         1555 a         1339 c         1294 g         1402 t
ORIGIN      
        1 atctacttca caatttaaat gagaaactaa aacttacatc ttgaaataat ctcattgatt
       61 ggatgaatat ttgtagtgca gtgacatcat ttttaaataa tagttcaaat aaaagggctc
      121 acgatgaaaa aattaacagt ggcaatttct gctgtagctg catcttaacg tataaactag
      181 atttaataat aacgaactag atttaataac aacgatatcc gtacctatcc ggatatctaa
      241 tgtcataagc atccctttaa ttatcccctc aacctcttgt aactttccca ctcttgaact
      301 ccacatagtc caaacgccgg atgcaaaaaa atcacacgtg catttctgaa attgtgatgg
      361 aggaagatag tatcaattat atgtgcttat aaacgtcccc gattagcgca gaagtcaccg
      421 gattgttcag gctccaatag gttgagtatg ccgtatttat tttccaaaat aaaaatcgat
      481 gctgttgctc cttcataaaa tacttttact ctctggacgc ttttcttagc tctcttggtg
      541 ttatcttaaa acgattatgg aacctttcag taaaacgaga aggacacttg taaccatttt
      601 ccctggcaat ctcgctgatg ggttttactg tcgtttgtat agcagacaac gcattattta
      661 acctcacgtc gtccaatata ctttgaaaac ttactccctc gcttgccaga cggcgatgca
      721 atgtggaaac agaaatgtag agatatctcg ctaccttgtt tgctgtccat tttgttccag
      781 gttcggatag cattaggtta taacaacgac ttattaatga ttgtttacta tctgataaaa
      841 gtaaacaatt aacatgatgt gctcctaatg aaagtagaac gcccattgct aagtgttcct
      901 gaatttgcgt tgagaagcct cgggaaatag atgtttttag ttgctcccaa caatatatta
      961 actcggaatt ctgaggtaag cagaaacctg tcgtgttacg aatttgatcg gttatcgcat
     1021 aaagattttg gaacttttca attagatcaa tgggataata aagcatttct gcaagataaa
     1081 gacctgcttc tgggtaattc gcaatataaa attcatatcc acagggaaat aatattatct
     1141 gattattatc aacagttaga ctatgcgtct cccaattgat aacttttttt ccctgacgga
     1201 tacgacatga agctggcata agaggcttaa ccctgtgaat ctcatgatgt ttatgcatcc
     1261 gtatttcttc aatctttagg ttagtcctat cttttgccag catactctta tcctacttta
     1321 cctcataaac gggtattatc tcagcggttg caattttatt ggcattaagc atataaccaa
     1381 ctaacgctcc gctggaatta gaatccacag gaatcttatc agtttttaga gcccatattt
     1441 taaactggta atgatggggt ttatctcctt tgggaggaca tgctccacca aatcccgcat
     1501 agccaaaatc atttcggcct tggacagcac cagtcggaag ttttgtacca tcacgtcttc
     1561 ctgcatcagc gggcaaatat gttaccgttg ctggaatatt agcaacagtc cagtgccacc
     1621 atccgctgcc tgtaggcgca tctggatcat acacagttac ggcaaagctt ttggtgcctt
     1681 ctggagcacc agaccaggtt aatgagggcg atgtattacc cccctcacac ccaactccag
     1741 aaaatacatg agacgttgta agttgctctc ctgtttttat ttcattacta gtgacctgaa
     1801 atgctgcggc ctgcgcagaa aatgttatga atgccaatac acttgaaacg ataagttttt
     1861 tcataaaaac ctctttgttg tgacctatcg ttattttatt tgatgtgcat tgatctcatt
     1921 atgcaaaaag gcgcaatgat cgcgcaaatg caatcataat tataaagttg ccccgaacat
     1981 tagccataat atgcacaaat cagattcctg ttatctcaag gatgattatt ttttaatgcg
     2041 gtgcgcttcg tgacaacaag ttaatgacta acacaccggc acaaatcaac atcatgccta
     2101 tgatggctgg caggtccagt cgttggccaa aaaatcccca ggaaagcaaa ctaatcagaa
     2161 cgataccgac tcctgaccag atagcataag caatccctgt aggtatataa gccagtgtct
     2221 gagctaataa ccaaaatgat gcacaatagc aaataattgt gccaacagag ggccataacc
     2281 gtgtaaaccc ctctgaaaac ttcattaagg ttgtaccaat gacctctgca agtattgcgc
     2341 caccaagata aatataaggg ttcatagcat attctttcct gttcatactg gagagaattg
     2401 tactacagtt tgaacacaac tcaccgtttt catcctggtg tatgtgtatc cagggggctc
     2461 tggcgtggca agagataaag gcaggaacaa taatgatcag aaggaacccc aggctaacaa
     2521 aagagttcac tcttcctatg agcaaagcga agacaaaaca tgttatcgcg tttacggatc
     2581 gtggatggca gaaaactttc aggatcaggt atccatttta aaccagaaat tgagtgagtt
     2641 tgccccatcc atgccccacg cggtgagatc ggatgtaatt aacaacaggt tacaaaacct
     2701 acacctgcat ggacatcact tcctgataag ccgccaccag cttattacgc acctgaatcc
     2761 ccatttgcat agaaactgag gctttttgca tatcggtcat cacatcgttt aacgccacgc
     2821 cgggttcacc gagagtgaat ttttctgcct gcgtgcgggc ggctgtttgt gtatcgctta
     2881 tgcgatcgag cgcggcgtgc agctgcccgg caaaactaat ggtcggttgt ggcagtgatt
     2941 cctgcgcacg cgcactcatc gccgtagcct gtaactggct gataacccct tcaatcccct
     3001 gtatcgctga cattttcatc tcccggataa tttctggtag caaagcttac cagtaagtca
     3061 ataagacaaa ggcgctaaat agcaacaaaa aaaacgggtt tattggcgga tagaaaaaaa
     3121 cgaaagcaca aataatggca gcgtcaattt ttcgagtttg ctgacccggg agtgagtctt
     3181 gttccacttt gccaataacg ccgtccataa tcagccacga ggtgcgcgat gaatgcgact
     3241 gcagcccaga caaaatctct tgagtggctt aatcgcctgc gtgcgaatcc gaaaattcca
     3301 ttgattgttg ccggttccgc ggcagtggcg gtcatggtcg cactgatcct gtgggcgaaa
     3361 gcccccgact accgcacatt attcagcaat ctttccgatc aggatggtgg cgcaattgtc
     3421 agccaactga cgcaaatgaa tattccttac tgcttcagcg aagccagcgg cgctattgaa
     3481 gttccggcag ataaagttca cgaactgcgt ctgcgcctgg cacaacaagg tttgcccaaa
     3541 ggcggcgcgg tcggtttcga actgcttgat caggaaaagt ttggtatcag ccagttcagc
     3601 gaacaggtga attatcagcg ggcgctggaa ggcgagcttt ctcgtaccat cgaaactatc
     3661 ggcccggtaa aaggggcgcg cgtacatctg gcaatgccga aaccgtcttt attcgtccgt
     3721 gaacaaaaat ccccttctgc atcggtgacg gtaaatctgt tacccggccg cgcactcgat
     3781 gaagggcaaa ttagcgccat tgtgcacctg gtttccagcg ccgttgctgg tctgccgccg
     3841 ggaaacgtca cgctggtgga tcagggcgga catctgttaa cccagtccaa taccagcggg
     3901 cgcgatctta atgacgctca gttgaaatat gccagcgatg tcgaaggccg tattcagcgg
     3961 cgtattgaag caatcctgtc gcctattgtt ggtaacggta atattcacgc ccaggtcacg
     4021 gcgcaactgg acttcgccag taaagaacaa acggaagaac agtatcgccc taacggtgat
     4081 gaatctcatg cggcgcttcg ttcacgccag cttaatgaga gcgagcaaag cggttccggt
     4141 tatccgggcg gcgtaccggg ggcgttgtcg aatcaaccgg cacctgcgaa taacgcgcca
     4201 atcagcacgc ctccggcaaa tcaaaataac cgccagcagc aggcgagcac caccagcaat
     4261 agcgggccgc gtagcacaca gcggaatgaa accagtaact acgaagtcga tcgcaccatt
     4321 cgtcatacca aaatgaacgt gggcgatgtg caacgtctgt cagtcgcggt agtggtgaat
     4381 tacaaaacct tgccagacgg taaaccattg cctctcagca acgaacagat gaagcaaatt
     4441 gaagatctga cccgcgaagc gatgggcttt tctgaaaaac gcggtgactc gctcaatgtc
     4501 gttaactcgc cgttcaatag cagtgacgaa agcggcggag aactgccgtt ctggcaacag
     4561 caagcgttta tcgatcagtt gctggctgcc ggtcgctggt tgctggtact gctggtggcg
     4621 tggctgctgt ggcggaaagc ggtacgcccg cagttaacac gtcgcgccga ggcgatgaaa
     4681 gctgtacagc aacaggcgca ggcccgtgag gaagtggaag atgcggtgga agtccgcctg
     4741 agcaaagacg aacaactcca acaacgacgc gctaaccaac gtctgggggc agaagtcatg
     4801 agccagcgta tccgtgaaat gtctgataac gatccgcgcg tggtggcgct ggtcattcgc
     4861 cagtggataa ataacgatca tgagtaacct gacaggcacc gataaaagcg tcatcctgct
     4921 gatgaccatt ggcgaagacc gggcggcaga ggtgttcaag cacctctccc agcgcgaagt
     4981 gcaaaccctg agcgctgcaa tggcgaacgt cacgcagatc tccaacaagc agctaaccta
     5041 tgtgctggcg gagtttgagc aagaagctga acagtttgcc gcactgaata tcaacgccaa
     5101 cgattatctg cgttcggtat tggtcaaagc tctgggtgag gaacgtgccg ccagcctgct
     5161 ggaagatatt ctcgaaactc gcgataccgc cagcggtatt gaaacgctca actttatgga
     5221 gccgcagagt gccgccgatc tgattcgcga tgagcatccg caaattatcg ccaccattct
     5281 ggtgcatctg aagcgcgcgc aggccgccga tattctggcg ttgttcgatg aacgtctgcg
     5341 ccacgacgtg atgttgcgta tcgccacctt tggcggcgtg cagccagccg cgctggcgga
     5401 actgaccgaa gtactgaatg gcttgctcga cggtcagaat ctcaagcgca gcaaaatggg
     5461 cggcgtgaga acggcagccg aaattatcaa cctgatgaaa actcagcagg aagaagccgt
     5521 tattaccgcc gtgcgcgaat tcgacggcga gctggcgcaa aaaattatcg acgagatgtt
     5581 cctgttcgaa
//
LOCUS       DAJTZL010000214         5526 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.215, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000214 DAJTZL010000000
VERSION     DAJTZL010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5526
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.215"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(33..770)
                     /gene="bamD"
                     /locus_tag="OO544_003724"
     CDS             complement(33..770)
                     /gene="bamD"
                     /locus_tag="OO544_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HCR6769225.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            905..1885
                     /gene="rluD"
                     /locus_tag="OO544_003725"
     CDS             905..1885
                     /gene="rluD"
                     /locus_tag="OO544_003725"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HCR6769226.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            1882..2613
                     /gene="pgeF"
                     /locus_tag="OO544_003726"
     CDS             1882..2613
                     /gene="pgeF"
                     /locus_tag="OO544_003726"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase"
                     /protein_id="HCR6769227.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAVHAGWRGLCAGVLEETVSCFADKPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2743..5316
                     /gene="clpB"
                     /locus_tag="OO544_003727"
     CDS             2743..5316
                     /gene="clpB"
                     /locus_tag="OO544_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HCR6769228.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
BASE COUNT         1361 a         1325 c         1503 g         1337 t
ORIGIN      
        1 caagggctgc cgttttgtgt ttcaggtttc tgttatgtat tgctgctgtt tgcggcgatg
       61 attttcgcta ctttttcagc ttgcgcattc atctgcatct gacggtatgc attttccatc
      121 agtggcagcg catcacgcgt agcctgggta tccgggtagt cgcgcaacat gccttctacg
      181 cggttaacga cggcaaccca tgcaccacgt tctgtatagt actcggcaac ggagtattca
      241 tatttcgcca gacgatcttt caggaatacc agacgtttgg tggcatcggt agtgtactga
      301 ctgttcggat agccgcgcac cagtttggaa aagtcactaa acgcagctcg tgcatgttga
      361 ggatcgcgat cgctacggtc aacgccaaag aacccttgca gcgcactgtc atccagcgcc
      421 atattggtca ggccacgcat gtacatgaca taatcgatat tcggatgggt cgggttaagg
      481 cgaataaaac gatcgatggc agcctgtgct aacggcaaat cggcgttttt atagtaggcg
      541 tagatgagat ccagctgcac ctgctgcgaa tacggaccaa acggatagcg attatctaac
      601 gcttccagtt gcgttattgc ctgtctccag ttaccgtcct gcagcttttg ttgtgcagtc
      661 gcgtaaattt catttggcgg attatcaggt acttcttcct ttgaccccga gcaacccgcc
      721 aaaaacaggc ttagtgtggc ggctgccacc agatatttca tgcgcgtcat gacgttttga
      781 ctttcctcaa aatgtaatac gggagattct ctgttcctgc tcccggttaa gaccagctac
      841 aatagcacac tatattaaac ggcaaagccg taaaacccca acgataaacg aagaagcagt
      901 atatatggca caacgagtac agctcactgc aacggtgtcc gaaaaccaac tcggtcaacg
      961 cttagatcag gctttggccg aaatgttccc ggattattca cgttcgcgga taaaagaatg
     1021 gatcctcgac cagcgcgtgc tggttaacgg caaagtttgt gataagccga aagaaaaagt
     1081 attgggtggc gagcaggttg ccatcaacgc tgagattgaa gaagaagcgc gttttgaacc
     1141 gcaggatatc ccgctggata tcgtctatga agatgaagat atcattgtca ttaataaacc
     1201 gcgcgaccta gtagtacatc ctggcgcggg taacccggat ggcacggtac tgaatgcgtt
     1261 gcttcattat tatccgccca ttgccgatgt accgcgtgcg ggcatcgtcc atcgtctgga
     1321 taaagacacc accggcctga tggttgtggc aaaaaccgtt ccggctcaga cgcgtttagt
     1381 cgaatctttg caacggcgtg aaattactcg tgagtatgaa gcggtggcga ttggtcatat
     1441 gaccgctggc ggtacagtag atgaacccat cagccgccac ccgaccaaac gtactcacat
     1501 ggcggtgcat ccgatgggca aaccggcggt gactcactat cgcatcatgg aacacttccg
     1561 tgtgcacacg cgtctgcggt tgcgtctgga aactggacgt acgcaccaga tccgcgtgca
     1621 tatggcccat atcactcatc cgctggtggg cgatccggtt tatggtggcc gtccgcgtcc
     1681 gccaaaaggt gcttcggaag catttatctc cacgctgcgt aagtttgacc gccaggcgct
     1741 tcatgcaacc atgctgcgtc tttatcaccc gatctctggc atcgaaatgg aatggcatgc
     1801 gcctattcca caagatatgg tggagctgat tgaggtgatg cgcgccgatt tcgaagagca
     1861 taaggatgaa gtggactggt tatgagtaag ctgattgtcc cgcagtggcc actgccaaaa
     1921 ggtgttgcgg cctgtagctc cactcgtatc ggcggcgtga gcttgccccc gtatgactca
     1981 ctcaacctcg gtgcccattg tggcgataac ccggatcacg ttgaggagaa tcgtaagcga
     2041 ctttttgctg cgggcaattt gccttctaaa ccggtctggc ttgagcaggt acacggcaaa
     2101 gatgtgctta agcttactgg cgaaccttat gcctcaaaac gagcagatgc ctcttatagc
     2161 aatacgcctg gcacggtttg cgcagtgatg actgccgact gcctccctgt gctgttttgc
     2221 aatcgagcgg gaacggaagt cgccgccgtt catgctggct ggcgtggact gtgcgcaggc
     2281 gtgctggaag agacggtttc ctgttttgct gataaacctg aaaacattct cgcctggtta
     2341 gggccggcaa ttggtccacg cgcgttcgaa gtgggggcgg aggttcgcga ggcgtttatg
     2401 gcagtagacg ctaaagcaag tgcagctttc attcagcatg gtgataagta tctggcggat
     2461 atttatcagc ttgcccggca gcgtctggcg aacgtgggtg ttgagcaaat tttcggcggc
     2521 gaccgttgta catatacgga aaatgagact ttcttctctt atcgtcgcga caagaccacc
     2581 ggtcgtatgg caagtttcat ttggctgata taacctaaag aatcaagacg atccggtacg
     2641 cgtgattttc ttttcacatt aatctggtca ataaccttga ataattgagg gatgacctca
     2701 tttaatctcc agtagcaact ttgatccgtt atgggaggag ttatgcgtct ggatcgtctt
     2761 actaataaat tccagcttgc tcttgccgat gcccaatcac ttgcactcgg gcacgacaac
     2821 caattcatcg aaccacttca tttaatgagc gccctgctga atcaggaagg gggttcggtt
     2881 agtcctttat taacatccgc tggcataaat gctggccagt tgcgcacaga tatcaatcag
     2941 gcattaaatc gtttaccgca ggttgaaggt actggtggtg atgtccagcc atcacaggat
     3001 ctggtgcgcg ttcttaatct ttgcgacaag ctggcgcaaa aacgaggtga taactttatc
     3061 tcgtcagaac tgttcgttct ggcggcactt gagtctcgcg gcacgctggc cgacatcctg
     3121 aaagcagcag gggcgaccac cgccaacatt actcaagcga ttgaacaaat gcgtggaggt
     3181 gaaagcgtga acgatcaagg tgctgaagac caacgtcagg ctttgaaaaa atataccatc
     3241 gaccttaccg aacgagccga acagggcaaa ctcgatccgg tgattggtcg tgatgaagaa
     3301 attcgccgta ccattcaggt gctgcaacgt cgtactaaaa ataacccggt actgattggt
     3361 gaacccggcg tcggtaaaac tgccatcgtt gaaggtctgg cgcagcgtat tatcaacggc
     3421 gaagtgccgg aaggattgaa aggccgccgc gtactggcgc tggatatggg cgcgctggtg
     3481 gctggggcga aatatcgcgg tgagtttgaa gaacgtttaa aaggcgtgct taacgatctt
     3541 gccaaacagg aaggcaacgt catcctgttt atcgacgaat tacataccat ggtcggcgcg
     3601 ggtaaagccg atggcgcaat ggacgccgga aacatgctga aaccggcgct ggcgcgtggt
     3661 gaattgcact gcgtaggtgc cacgacgctt gacgaatatc gccagtacat tgaaaaagat
     3721 gctgcgctgg aacgtcgttt ccagaaagtg tttgttgccg agccttctgt tgaagatacc
     3781 attgcgattc tgcgtggcct gaaagaacgt tacgaattgc accaccatgt gcaaattact
     3841 gacccggcaa ttgttgcagc ggcgacgttg tctcatcgct acattgctga ccgtcagctg
     3901 ccggataaag ccatcgacct gatcgatgaa gcagcatcca gcattcgtat gcagattgac
     3961 tcaaaaccag aagaactcga ccgactcgat cgtcgtatca tccagctcaa actggaacaa
     4021 caggcgttaa tgaaagagtc tgatgaagcc agtaaaaaac gcttggatat gctcaacgaa
     4081 gaactgagcg acaaagaacg tcagtactcc gagttagaag aagagtggaa agcagagaag
     4141 gcatcgcttt ctggtacgca gaccattaaa gcggaactgg aacaggcgaa aatcgctatt
     4201 gaacaggctc gccgtgtggg ggacctggcg cggatgtctg aactgcaata cggcaaaatc
     4261 ccggaactgg aaaagcaact ggaagccgca acgcagctcg aaggcaaaac tatgcgtctg
     4321 ttgcgtaata aagtgaccga cgccgaaatt gctgaagtgc tggcgcgttg gacggggatt
     4381 ccggtttctc gcatgatgga aagcgagcgc gaaaaactgc tgcgtatgga gcaagaactg
     4441 caccatcgcg tcattggtca gaacgaagcg gttgatgcgg tatctaacgc tattcgccgt
     4501 agccgtgcgg ggttggcgga tccaaatcgc ccgattggtt cattcctgtt cctcggccca
     4561 acgggtgtgg ggaaaactga gctttgtaag gcgctggcga actttatgtt tgatagcgac
     4621 gaggcgatgg tccgtatcga tatgtccgag tttatggaga aacactcggt gtctcgtctg
     4681 gttggtgcgc ctccgggata tgtcggttat gaagaaggtg gctacctgac cgaagctgtg
     4741 cgtcgtcgtc cgtattccgt catcctgctg gatgaagtgg aaaaagcgca tccggatgtc
     4801 ttcaacattc tgttgcaggt actggatgat ggtcgtctga ctgacgggca ggggagaacg
     4861 gtcgacttcc gtaatacggt tgtcattatg acctctaacc tcggttccga tttgattcag
     4921 gaacgcttcg gtgaactgga ttatgcgcac atgaaagagc tggtgctcgg tgtggtaagc
     4981 cataacttcc gtccggaatt cattaaccgt attgatgaag tggtggtctt ccatccgctg
     5041 ggtgaacagc acattgcctc gattgcgcag attcagttga aacgtctgta caaacgtctg
     5101 gaagaacgtg gttatgaaat ccacatttct gacgaggcgc tgaaactgct gagcgagaac
     5161 ggttacgatc cagtctatgg tgcacgtcct ttgaaacgtg ctatccagca gcagatcgaa
     5221 aacccgctgg cacagcaaat actgtctggt gaattggttc cgggtaaagt gattcgcctg
     5281 gaagttaatg aagaccggat tgtcgctgtc cagtaatgat aaaacgagcc cttcggggct
     5341 cgtttttgtc tataagttag acggaaaaga ctatatttaa gatgttttgc ctgaaaaatg
     5401 agcgaacgat aaagttttta tatttttcgc ttgtcaggcc ggaataactc cctataatgc
     5461 gccaccactg acacggaaca acggcaaaca cgccgccggg tcggcggggt tctcctgaga
     5521 attccg
//
LOCUS       DAJTZL010000215         5514 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.216, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000215 DAJTZL010000000
VERSION     DAJTZL010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5514
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.216"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(18..93)
                     /locus_tag="OO544_003728"
     tRNA            complement(18..93)
                     /locus_tag="OO544_003728"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:His,seq:gtg)
     gene            complement(152..228)
                     /locus_tag="OO544_003729"
     tRNA            complement(152..228)
                     /locus_tag="OO544_003729"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(192..194),aa:Arg,seq:ccg)
     gene            complement(329..1714)
                     /locus_tag="OO544_003730"
     CDS             complement(329..1714)
                     /locus_tag="OO544_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR6769229.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(1905..2645)
                     /gene="rffM"
                     /locus_tag="OO544_003731"
     CDS             complement(1905..2645)
                     /gene="rffM"
                     /locus_tag="OO544_003731"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HCR6769230.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(2648..4000)
                     /gene="wzyE"
                     /locus_tag="OO544_003732"
     CDS             complement(2648..4000)
                     /gene="wzyE"
                     /locus_tag="OO544_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HCR6769231.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNREPNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(3997..5076)
                     /gene="rffT"
                     /locus_tag="OO544_003733"
                     /gene_synonym="wecF"
     CDS             complement(3997..5076)
                     /gene="rffT"
                     /locus_tag="OO544_003733"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HCR6769232.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNDHIAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(5073..>5514)
                     /locus_tag="OO544_003734"
     CDS             complement(5073..>5514)
                     /locus_tag="OO544_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="involved in the movement of lipid III across the
                     membrane; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HCR6769233.1"
                     /translation="SFSVYLLPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLL
                     RDFAIWLLLSNKFTAMRDLFAWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFT
                     LLMVFAHWLIPAHGALGAAQAYMATYIVYFSLCCGVFLLWRRRA"
BASE COUNT         1525 a         1543 c         1340 g         1106 t
ORIGIN      
        1 gtcacaactt ctaataatgg ggtggctaat gggattcgaa cccacgacaa ctggaatcac
       61 aatccagggc tctaccaact gagctatagc caccactaca aatcttgtta cgcggtatta
      121 ctaccaccgc agctcaagcg ccgggactaa atggcgcgcc cgacaggatt cgaacctgag
      181 acctctgcct ccggagggca gcgctctatc cagctgagct acgggcgctt agcgccgttg
      241 cggggggata atacggactt cccacccctc tgtctagtcc ctttttaaat aaattgcgcg
      301 tttggttacg gtttgtgcgt tttgctgctt attcctccag tttatgcgct ttcccgtggc
      361 gattaaggcc aaaaacttta taaatcgccg tcaccgccag cataaagatg atgccaacaa
      421 acagcgacat acgcgtatct tcattaaagt acatgccgat caaaacgcaa atcaggaatg
      481 ccattgttac gtagttggcc cacgggaaca ggattgagcg gaacggatgg ctggcaatcg
      541 ccgctttatg tgcacgccga aaacgcagct ggcttatcaa tatcacaaac catggcacca
      601 tccccggaag cacgctggca ctgtagacgt agacaaacac acgctgcgga ttgggaatga
      661 tgtagttcag gcatgagcca attagcagaa tagcaataga tactgccaca cccgcaactg
      721 gtacgccgtg acgggaaact ttcgccatcg ccgccggtaa ctgacggttt ttcgccagtg
      781 cgtagagcat acgtccgcaa ctgtacatgc cgctgttaca gccagagagc gcagccgtca
      841 gcaccacaaa gttgataatg cccgccgctg cggtaatacc gattttggca aaagtcagta
      901 cgaacgggct gccgttgctg ccaatttcat tccacgggaa gatggtgacg ataacgaaaa
      961 tcgcgcctac gtagaaaatc aggatccgcc acagcacttt gcctacagca ctgcgcagcg
     1021 tcacctgcgg attcttcgct tcaccggcag taatgccaat cagctccacg ccctggtagg
     1081 acgccaccac aatacacagc gcggtcagga accctttcca gccacccgca aagaaaccgc
     1141 catgctctgt gagattgcta aaaccaatcg actgcccgcc attgccaaag ccaaagaaaa
     1201 tcacgcccag gccaatgaca atcatcacga taatcgtggt gactttgatc atcgcgaacc
     1261 agaactcgat ttcgccgtac aaccgcaccg ccgccagatt cgccaacgcc accagcgcca
     1321 ccgcgatcaa tgcaggtatc cactgcgcca tctccgggaa ccagaactgg acataaacgc
     1381 caatggcggt gatttctgag atccccaccg ccatccacat aaaccagtaa gaccaggcgg
     1441 tgagataacc aaaaaacggg ctcatataac gatgcgcata aacggcgaac gaaccggtaa
     1501 caggttcaag gaacaacatt tcgcccattg aacgcatgat gaaaaagacg aacagcccgg
     1561 cgatgatata ggccaacaat acggatggcc cggcccattt cagggtactg gcggccccca
     1621 taaacaggcc gacgccaata gtgcccccca gggcgatgag ttcgatatgt cgagcttcca
     1681 gcccacgctg tagctctggt ttgttatctg ccataaatcc tcgtgttgtg tttgcatgct
     1741 ttccggtgtt accggttatc gttatgggta catcgagtgt tgcgaatgtt ttcgtaattc
     1801 aggagaaatg gcaaataaag cattaaaaat ttgaatgctt tgtgtaataa aaaagcagac
     1861 aggcgacgga gtgaccactc cgtcgcttta caaagagagg aaaatcagag gttgccggtg
     1921 tagtgccagc gtaaataacg cagcaaacga agctgacgct taatgcggct cggctgcgaa
     1981 agcaggcggt agagccactc cagtcccagc gtttgccaga ttttcggtgc gcgttttacg
     2041 tgaccggtga aaacatcgta agtcccgcca acgcccatat acagcgcatc tggatgcacc
     2101 agacggcagt cgcgcatgaa gatctcctgc tttggcgatc ccatcgcaac ggtgacgatt
     2161 tgcgctccgc tggcatgaat gcgttcaaac agcgcctgac gctgctcggg tttaaaataa
     2221 ccatcctgac tgccaacgat attcacattc cattggttgc gcagtttagc ttcagtttgc
     2281 gccagcactt caggtttacc gcccacaaga aataccggcg tcccttcttt gcctgcgcgc
     2341 gccatcagct cttcccagag atcggcaccg gcaacgcggg aaacctgcgc ctgcgggtac
     2401 tttttacgta ctgaacgtac aacgctgatg ccatccgcat atttaaattc ggcagcgtta
     2461 attaactccc tgacctcggc gttatcttca atggtcagca ttttttcagc attaatggca
     2521 accagcgttc cctgcttaag ctgcccgtca gcaaacagat aatcgagggc gtgctgcata
     2581 tcacgccaac caatcaactg taagccacgc agcgtatagg tcggtgccgt ggtgttgtta
     2641 ttcattgtta tccttcaacc tgcgtccgga gcgatgattt tgtacgttta tgaatgagtc
     2701 cggcgctttc aaaaagccag tacagcagtt ttgcgatcat cagacatgcg ccgaagacca
     2761 cgataaaaaa gactacgcgt gagacaaacg aatccagccc ttcacgcgcc agcacgatca
     2821 tattgaagat cgccccaaag cagaaactgt gcaatatcgc agccttatag cgattaggct
     2881 cgcggttgcc cagctcatac agccagtcga accatttgat gatcagacca accacgatcg
     2941 ccccgagcgg gatgaacaac gcgccgccca tcaccaccag tgagcctata agcgtaggcg
     3001 agatcgccag tccggagtgg ttattcagca cttcccaggt aaagtagttg gctgagttca
     3061 gcaccatact cgggcgaccc ggccacagcc aggaagggat aaagacatag aaatcgcgga
     3121 caattggagc caggccctgg aagtcgatgt tgtcgtagtt ctgcaacagc aacgccagat
     3181 tctcccacgg ggagaaggtg tcgcgagtga gatagagaaa cgtatagaac gcttcatcgc
     3241 cgctcacatt cattccatag cgttttagcg ccagccagaa catgccaaca atccccagca
     3301 cgcccgccgc cgccagcatc cacaacgaaa tccagccgcg aataatgcca ataaacagga
     3361 agatagcgaa tgcgatgatg atattggcgc gagtgccgcc gacaatcata taagtcagca
     3421 agccaaaggc gaccgtgctg acgaggaaaa acagccacgc tttgctgtcc tggcgcagaa
     3481 agtagaccac cagcatcgcc gggatgaaaa agtaaaagaa gcgttttaac gccacgccgg
     3541 agacttcact ggagaagatc tgactgtagg agttaagccg gaacagcaaa aagccgttgt
     3601 gcatgaagaa gatgccgacg cttaccagcg cgatacccat caggatcacc cacgtaagat
     3661 tggtctccac gcggttcatg gtaaacagcg gacggcgcgg tacatcagca acgcgtttgc
     3721 gtaggcgggt tttgtaggtg acatagtaaa ccgcgtagaa gcagcccgca gaaagcaacg
     3781 cctgcaacaa gatttctggc ggcgcgacac caacatcaaa gcgaaatacc agcacgctgg
     3841 tcagcgggaa gccgaagaaa aaggtgagca aaaacagcaa tgaaaagaag acattgaagt
     3901 taaagcgcac acggcgaaac tcaaaccagg tcagcgtggc aataaacagc gtgcagagca
     3961 gccagaccac aaacaggcca ctgaattgca gcagactcat gcgacctccc cggcggcaat
     4021 agccaacgcc cgctgccagc cttgtagata gttagggcta aagaaggcaa tggtgttttt
     4081 atccaccgac gccaactggc gctgcgcttc acgcacaata tcctcgttga gatcgtcggt
     4141 agtaaacagc accggcaaat gctgttccgt catatcctgc cagaacggat tttcccggtt
     4201 aagcacacaa ggaatgcccg cctgaatcag taagcaaagc gtaccaatgc cctgctggcg
     4261 ggcaaaaata aagtaaccaa gatcgcactg acgaagtagc gccagatagg cgtcaaattc
     4321 cagtttttcg ctcaaaattt gcagattttc ttcgctgaat aactccagcc ccgcctgacg
     4381 aacttcctca atgtacgctt cgttattagg cggatatccc atcggcacca ccacttttac
     4441 cgtatcgcca aattgctgat gaacggcgcg caaggcagca atatggtcat tgctgcggtc
     4501 accggagttc cccaccagaa tggtcatttt cccttcacgt tgccgatcat tcgccatcgt
     4561 attgagagaa gggtccatcc gcgtcgggaa gtacagcagt tcgccccgca cctttggatg
     4621 cgttttggca aaaaagctca aatcaccgcg ggtggcaaat acacagccga ctcgcttttg
     4681 cgccaggcga cgtagcgggt aaaaaagctt atatctcaag ccactggaaa gctcatacag
     4741 gtctgccccc cagatatgcc agaaaaactg gctgggctta atgccaccac tcagcagagc
     4801 cagccacagt gtgggattga actgaccgtg gaaaaaaaat cgctgctgac ggttagcttt
     4861 tgctttcgcg atgaccgctt ccgccagcga ttttttccca gggaaaaatt gcacagaaag
     4921 cgccggacag ctatcactta agccgtcgtc cttgccaaca accataaact cgcgcgcgtg
     4981 ctcgctcgtc gcggccagcg cgtcattgaa aaaccgcaaa acggttcggt tatggtgagg
     5041 gatatccgat cccagtacgt gaatcagtac agtcatgccc gcctacgcca gagtaaaaac
     5101 acgccacaac aaagagaaaa atagacgata taagttgcca tatatgcctg cgccgcgccc
     5161 agtgcaccat gcgcagggat tagccagtgg gcaaatacca tcaataaagt gaactggctg
     5221 acttccgcca gaatataaaa ccgcagtgac gctttggcga tcaccagata accaaagaca
     5281 taagcgccca cttttaacac atcacccact aactgccagg caaagagatc gcgcatagcg
     5341 gtaaatttat tcgacaacag caaccagata gcaaaatcac gcagcaacca gacggtaaaa
     5401 ctcgccgccg ccactgccgg taagacgaat ttcagcgatt taaccacttc ccgggtgata
     5461 tcgcgctttt ccgttagccg cgacaacgtg ggcagcaaat atacgctgaa cgat
//
LOCUS       DAJTZL010000216         5486 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.217, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000216 DAJTZL010000000
VERSION     DAJTZL010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5486
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.217"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..348)
                     /gene="zipA"
                     /locus_tag="OO544_003735"
     CDS             complement(<1..348)
                     /gene="zipA"
                     /locus_tag="OO544_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HCR6769234.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPIQQPPEAQVPPQ"
     gene            578..1339
                     /gene="cysZ"
                     /locus_tag="OO544_003736"
     CDS             578..1339
                     /gene="cysZ"
                     /locus_tag="OO544_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HCR6769235.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            1524..2495
                     /gene="cysK"
                     /locus_tag="OO544_003737"
     CDS             1524..2495
                     /gene="cysK"
                     /locus_tag="OO544_003737"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HCR6769236.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            2879..3136
                     /gene="ptsH"
                     /locus_tag="OO544_003738"
     CDS             2879..3136
                     /gene="ptsH"
                     /locus_tag="OO544_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HCR6769237.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            3181..4908
                     /gene="ptsI"
                     /locus_tag="OO544_003739"
     CDS             3181..4908
                     /gene="ptsI"
                     /locus_tag="OO544_003739"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HCR6769238.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            4949..5458
                     /gene="crr"
                     /locus_tag="OO544_003740"
     CDS             4949..5458
                     /gene="crr"
                     /locus_tag="OO544_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR6769239.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
BASE COUNT         1411 a         1306 c         1365 g         1404 t
ORIGIN      
        1 ttgcggcggt acctgcgctt caggcggctg ctggatcggt tgacgcggct gcgcagacgc
       61 ataaggcggt tggtactggt gttgcggcga cggacgagca gcctcatgct cctgagcgtt
      121 agccggggca tgattcacgc ggtgaacacg aacctcacca acgccctcat catcttcgac
      181 atcctcgtca taagaatcgt cgtcacgttt tgacttcatt cgttttaatg gccgatcgcg
      241 gaacatagaa gatcgttctt tacggctggt ccagaaacca tgtaccagta aagcgattat
      301 ggcgatcgcg ccaacaatga ttaatatcag acgcaaatcc tgcatcatta tattctctgt
      361 tgttctaaca ccttgccacc acggcaaaca tttactcact aagagtattt gccgattacc
      421 tcaagtgcaa gtgcactatt aactttcaca gcacaaagat agatgaaatc gtgctttttg
      481 ctgttttttc gaacatatcc taactgtcca ttgcgcaatt acccggtaaa atacgcagaa
      541 ttttcctggg attggtcaaa aggagctcat cctgattatg gtttcatcat tcacatctgc
      601 cccacgcagc ggtttttact attttgcgca aggctggaag ctcgtctcgc aacctgggat
      661 tcggcgtttc gttattttac cgctgctggt caatattttg ttgatggggg gcgcattctg
      721 gtggctcttt acacagctcg atgtctggat cccgactctc atgagttacg ttccggactg
      781 gctacaatgg ctgagttatc tgttgtggcc tctggctgtc atctctgtgc tgttagtgtt
      841 tggctatttc ttctccacga ttgctaactg gattgccgct ccgtttaacg gtttattggc
      901 tgaacaactg gaagcacgat tgactggcgc tacaccgcca gataccggga ttttcggtat
      961 catgaaagat gtgccgcgaa tcatgaaacg cgaatggcaa aaatttgcct ggtatctgcc
     1021 gcgcgcaatt gtattactaa ttctttactt catccctggt attgggcaaa ccgtcgcgcc
     1081 ggtactgtgg ttcctgttta gcgcctggat gttagccatc cagtattgcg attacccctt
     1141 cgataaccac aaagtgccgt ttaaagagat gcgcaccgcc ctgcgcacac gcaaaatcac
     1201 caatatgcag tttggtgctt taaccagcct gtttacgatg atcccgctgc ttaatctgtt
     1261 catcatgccc gttgccgttt gtggcgcgac ggcgatgtgg gtcgattgct atcgcgataa
     1321 acacgcgatg tggcggtaac aatctaccgg ttattttgta aaccgtttgt gtgaaacagg
     1381 ggtggcttat gccgcccctt attccatctt gcatgtcatt atttcccttc tgtatataga
     1441 tatgctaaat ccttacttcc gcatattctc tgagcgggta tgctacctgt tgtatcccaa
     1501 tttcatacag ttaaggacag gccatgagta agatttttga agataactcg ctgactatcg
     1561 gtcacacgcc gctggttcgc ctgaatcgca tcggtaacgg acgcattctg gcgaaggtgg
     1621 aatctcgtaa ccccagcttc agcgttaagt gccgtatcgg tgccaacatg atttgggatg
     1681 ccgaaaagcg cggcgtgctg aaaccaggcg ttgaactggt tgaaccgacc agcggtaata
     1741 ccgggattgc actggcctat gtagctgccg ctcgcggtta caaactcacc ctgaccatgc
     1801 cagaaaccat gagtattgaa cgccgcaagc tgctgaaagc gttaggtgca aacctggtgc
     1861 tgacggaagg tgctaaaggc atgaaaggcg caatccaaaa agcagaagaa attgtcgcca
     1921 gcaatccaga gaaatacctg ctgctgcaac aattcagcaa tccggcaaac cctgaaattc
     1981 acgaaaaaac caccggtccg gagatctggg aagataccga cggtcaggtt gatgtattta
     2041 ttgctggcgt tgggactggc ggtacgctga ctggcgtcag ccgctacatt aaaggcacca
     2101 aaggcaagac cgatcttatc tctgtcgccg ttgagccaac cgattctcca gttatcgccc
     2161 aggcgctggc aggtgaagag attaaacctg gcccgcataa aattcagggt attggcgctg
     2221 gttttatccc ggctaacctc gatctcaagc tggtcgataa agtcattggc atcaccaatg
     2281 aagaagccat ttctaccgcc cgtcgtctaa tggaagaaga aggtattctt gcaggtatct
     2341 cttctggagc agctgttgcc gcggcgttga aactacaaga agatgaaagc tttaccaata
     2401 agaatattgt ggttattcta ccatcatcgg gtgagcgtta tttaagcacc gcattgtttg
     2461 ccgatctctt cactgagaaa gaattgcaac agtaatgcca gcttgttaaa aatgcgtaaa
     2521 aaagcacctt tttaggtgct tttttgtggc ctgcttcaaa ctttcgcccc tcctggcatt
     2581 gattcagcct gtcggaactg gtatttaacc agactaatta ttttgatgcg cgaaattaat
     2641 cgttacagga aaagccaaag ctgaatcgat tttatgattt ggttcaattc ttcctttaac
     2701 ggcataatgt ttaatgacgt acgaaacgtc agcggtcaac acccgccagc aatggactgt
     2761 attgcgctct tcgtgcgtcg cgtctgttaa aaactggcgc taacaataca ggctaaagtc
     2821 gaaccgccag gctagacttt agttccacaa cactaaacct ataagttggg gaaatacaat
     2881 gttccagcaa gaagttacca ttaccgctcc gaacggtctg cacacccgcc ctgctgccca
     2941 gtttgtaaaa gaagctaagg gcttcacttc tgaaattact gtgacttcca acggcaaaag
     3001 cgccagcgcg aaaagcctgt ttaaactgca gactctgggc ctgactcaag gtaccgttgt
     3061 gactatctcc gcagaaggcg aagacgagca gaaagcggtt gaacatctgg ttaaactgat
     3121 ggcggaactc gagtaatttc ccgggttctt ttaaaaatca gtcacaagta aggtagggtt
     3181 atgatttcag gcattttagc atccccgggt atcgctttcg gtaaagctct gcttctgaaa
     3241 gaagacgaaa ttgtcattga ccggaaaaaa atttctgccg accaggttga tcaggaagtt
     3301 gaacgttttc tgagcggtcg tgccaaggca tcagcccagc tggaaacgat caaaacgaaa
     3361 gctggtgaaa cgttcggtga agaaaaagaa gccatctttg aagggcatat tatgctgctc
     3421 gaagatgagg agctggagca ggaaatcata gccctgatta aagataaaca catgacagct
     3481 gacgcagctg ctcatgaagt tatcgaaggt caggcttctg ccctggaaga gctggatgat
     3541 gaatacctga aagaacgtgc ggctgacgta cgtgatatcg gtaagcgcct gctgcgcaac
     3601 atcctgggcc tgaagattat cgacctgagc gccattcagg atgaagtcat tctggttgcc
     3661 gctgacctga cgccgtccga aaccgcacag ctgaacctga agaaggtgct gggtttcatc
     3721 accgacgcgg gtggccgtac ttcccacacc tctatcatgg cgcgttctct ggaactgcct
     3781 gctatcgtgg gtaccggtag cgtcacctct caggtgaaaa atgacgacta tctgattctg
     3841 gatgccgtaa ataatcaggt ttacgtcaat ccaaccaacg aagttattga taaaatgcgc
     3901 gctgttcagg agcaagtggc ttctgaaaaa gcagagcttg ctaaactgaa agatctgcca
     3961 gctattacgc tggacggtca ccaggtagaa gtatgcgcta acattggtac ggttcgtgac
     4021 gttgaaggtg cagagcgtaa cggcgctgaa ggcgttggtc tgtatcgtac tgagttcctg
     4081 ttcatggacc gcgacgcgct gcccactgaa gaagaacagt ttgctgctta caaagcagtg
     4141 gctgaagcgt gtggctcgca ggcggttatc gttcgtacca tggacatcgg cggcgacaaa
     4201 gagctgccat acatgaactt cccgaaagaa gagaacccgt tcctcggctg gcgcgctatc
     4261 cgtatcgcga tggatcgtaa agagatcctg cgcgatcagc tccgcgctat cctgcgtgcc
     4321 tcggctttcg gtaaattgcg cattatgttc ccgatgatca tctctgttga agaagtgcgt
     4381 gcactgcgca aagagatcga aatctacaaa caggaactgc gcgacgaagg taaagcgttt
     4441 gacgagtcaa ttgaaatcgg cgtaatggtg gaaacaccgg ctgccgcaac aattgcacgt
     4501 catttagcca aagaagttga tttctttagt atcggcacca atgatttaac gcagtacact
     4561 ctggcagttg accgtggtaa tgatatgatt tcacaccttt accagccaat gtcaccgtcc
     4621 gtgctgaact tgatcaagca agttattgat gcttctcatg ctgaaggcaa atggactggc
     4681 atgtgtggtg agcttgctgg cgatgaacgt gctacacttc tgttgctggg gatgggtctg
     4741 gacgaattct ctatgagcgc catttctatc ccgcgcatta agaagattat ccgtaacacg
     4801 aacttcgaag atgcgaaggt gttagcagag caggctcttg ctcaaccgac aacggacgag
     4861 ttaatgacgc tggttaacaa gttcattgaa gaaaaaacaa tctgctaatc cacgagatgc
     4921 ggcccaattt actgcttagg agaagatcat gggtttgttc gataaactga aatctctggt
     4981 ttccgacgac aagaaggata ccggaactat tgagatcatt gctccgctct ctggcgagat
     5041 cgtcaatatc gaagacgtgc cggatgtcgt ttttgcggaa aaaatcgttg gtgatggtat
     5101 tgctatcaaa ccaacgggta acaaaatggt cgcgcctgta gacggtacca ttggtaaaat
     5161 ctttgaaacc aatcacgcgt tctctatcga atctgatagc ggcgttgaac tgttcgtcca
     5221 cttcggtatc gacaccgttg aactgaaagg cgaaggcttc aagcgtattg ctgaagaagg
     5281 tcagcgcgtg aaagttggcg atactgtcat tgaatttgat ctgccgctgc tggaagagaa
     5341 agccaagtct accctgactc cggttgttat ctccaacatg gacgaaatca aagaactgat
     5401 caaactgtcc ggtagcgtaa ccgtgggtga aaccccggtt atccgcatca agaagtaatt
     5461 cttgccgcag tgaaaaatgg cgccca
//
LOCUS       DAJTZL010000217         5477 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.218, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000217 DAJTZL010000000
VERSION     DAJTZL010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5477
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.218"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(278..649)
                     /locus_tag="OO544_003741"
     CDS             complement(278..649)
                     /locus_tag="OO544_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6769240.1"
                     /translation="MSYEPPRDGYTNGYIVDVMYDNRPSKYPAVFRTYVRIPDYVELD
                     NAEELADAIIREVELKEAWLTRVLGNSADPDASVGKWDRRLAQCGGIDEMSSGVQATY
                     FSPNGQLVGLVAYIDAEWMDE"
     gene            complement(710..1207)
                     /locus_tag="OO544_003742"
     CDS             complement(710..1207)
                     /locus_tag="OO544_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6769241.1"
                     /translation="MRDLLKVVLSLVLAMASGQALADLPIVLVDEARLPYDYSPSNYD
                     ISPSNYDNSISNYDNSPSNYDNSESNYDNSSSNYDNSRNGNRRLIYSANGSRTFAGYY
                     VIANNGTTNFFSTSGKRMFYTPKGGRGVYGGKDGSFCGALVVINGQFSLALTDNGLKI
                     MYLSN"
     gene            complement(1283..2071)
                     /gene="aadA1"
                     /locus_tag="OO544_003743"
     CDS             complement(1283..2071)
                     /gene="aadA1"
                     /locus_tag="OO544_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCR6769242.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
     gene            complement(2129..2653)
                     /gene="sat2"
                     /locus_tag="OO544_003744"
     CDS             complement(2129..2653)
                     /gene="sat2"
                     /locus_tag="OO544_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HCR6769243.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            complement(2748..3221)
                     /locus_tag="OO544_003745"
     CDS             complement(2748..3221)
                     /locus_tag="OO544_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HCR6769244.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            3553..4089
                     /locus_tag="OO544_003746"
     CDS             3553..4089
                     /locus_tag="OO544_003746"
                     /inference="COORDINATES: protein motif:HMM:NF024886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 2 integron integrase IntI2"
                     /protein_id="HCR6769245.1"
                     /translation="MSNSPFLNSIRTDMRQKGYALKTEKTYLHWIKRFILFHKKRHPQ
                     TMGSEEVRLFLSSLANSRHVAINTQKIALNALAFLYNRFLQQPLGDIDYIPASKPRRL
                     PSVISANEVQRILQVMDTRNQVIFTLLYGAGLRINECLRLRVKDFDFDNGCITVHDGK
                     GGKSRNSLLPTRLIPAIK"
     gene            4204..4530
                     /locus_tag="OO544_003747"
     CDS             4204..4530
                     /locus_tag="OO544_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6769246.1"
                     /translation="MFVFPSSTLCNHPYNGKLCRHHLHDSVARKALKAAVQKAGIVSK
                     RVTCHTFRHSFATHLLQAGRDIRTVQELLGHNDVKTTQIYTHVLGQHFAGTTSPADGL
                     MLLINQ"
     gene            complement(4718..4957)
                     /locus_tag="OO544_003748"
     CDS             complement(4718..4957)
                     /locus_tag="OO544_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HCR6769247.1"
                     /translation="MTPFRYNSDLTSGSLQTRECRIITGLLLQELDEAAWDKAMYKEN
                     VLQKRTQSTVRRISSALRKRLEHLSSDFWAFAFLC"
BASE COUNT         1426 a         1306 c         1219 g         1526 t
ORIGIN      
        1 ctctctagat agcagggatt tcttggcctt gcctcatgct gatagcttgt aaatatcaca
       61 cgatccttag ccccacaaag tgagcatact ccgtagcttt catttataga aatcgtcgtt
      121 agattgtcgc agcatgtgca cgttccgact atttgctcag gaagcatcgc cataccttgg
      181 cctaggtaaa gaagatcaga ctcgtagcca catcggtggc aactgagctt gtacgcaatg
      241 cccatctggt tgtctcacct gaatcgtaac gtcagagcta ttcatccatc cattcggcat
      301 caatgtatgc cactaagccg accagctgac cgtttggact gaagtatgtt gcctgcaccc
      361 cacttgacat ctcatcaata ccaccgcact gcgctaggcg ccggtcccac ttgcccacag
      421 aagcgtcagg atcggctgag ttccccaaga cccttgtgag ccatgcctcc tttaactcaa
      481 cctctcgaat gattgcgtcg gccagctcct ccgcgttatc tagttcgaca tagtctggaa
      541 ttcggacgta cgttctgaac acagctggat acttacttgg gcgattgtca tacatgacat
      601 caacaatgta cccgtttgtg taaccgtctc ttggaggttc gtatgacact agtggttccc
      661 ctcagcttgc gactagatgt tgaggcctaa cattttatta gagagcaggc tagttgctta
      721 gatacatgat cttcaggccg ttatctgtca gggcaagcga aaattggcca tttatgacga
      781 ccaatgcccc gcagaagctc ccatctttgc cgccatagac gccgcgcccc ccttttgggg
      841 tgtagaacat ccttttgcca gatgtggaaa agaagttcgt tgtcccattg ttggcaatga
      901 cgtagtagcc ggcgaaagtg cgagacccat ttgcgctata tataagccta cgatttccgt
      961 tgcgactatt gtcgtaattg gatgaactat tatcgtagtt gctctcagag ttgtcgtaat
     1021 ttgatggact attgtcgtaa ttgcttatgg agttgtcgta gttgcttgga gaaatgtcgt
     1081 agttggatgg ggagtagtca tagggaagac gagcttcatc cactaaaaca attggcaggt
     1141 cagcaagtgc ctgccccgat gccatcgcaa gtacgaggct tagaaccacc ttcaacagat
     1201 cgcgcatagt cttccccagc tctctaacgc ttgagttaag ccgcgccgcg aagcggcgtc
     1261 ggcttgaacg aattgttaga cattatttgc cgactacctt ggtgatctcg cctttcacgt
     1321 agtgaacaaa ttcttccaac tgatctgcgc gcgaggccaa gcgatcttct tgtccaagat
     1381 aagcctgcct agcttcaagt atgacgggct gatactgggc cggcaggcgc tccattgccc
     1441 agtcggcagc gacatccttc ggcgcgattt tgccggttac tgcgctgtac caaatgcggg
     1501 acaacgtaag cactacattt cgctcatcgc cagcccagtc gggcggcgag ttccatagcg
     1561 ttaaggtttc atttagcgcc tcaaatagat cctgttcagg aaccggatca aagagttcct
     1621 ccgccgctgg acctaccaag gcaacgctat gttctcttgc ttttgtcagc aagatagcca
     1681 gatcaatgtc gatcgtggct ggctcgaaga tacctgcaag aatgtcattg cgctgccatt
     1741 ctccaaattg cagttcgcgc ttagctggat aacgccacgg aatgatgtcg tcgtgcacaa
     1801 caatggtgac ttctacagcg cggagaatct cgctctctcc aggggaagcc gaagtttcca
     1861 aaaggtcgtt gatcaaagct cgccgcgttg tttcatcaag ccttacggtc accgtaacca
     1921 gcaaatcaat atcactgtgt ggcttcaggc cgccatccac tgcggagccg tacaaatgta
     1981 cggccagcaa cgtcggttcg agatggcgct cgatgacgcc aactacctct gatagttgag
     2041 tcgatacttc ggcgatcacc gcttccctca tgatgtttaa ctcctgaatt aagccgcgcc
     2101 gcgaagcggt gtcggcttga atgaattgtt aggcgtcatc ctgtgctccc gagaaccagt
     2161 accagtacat cgctgtttcg ttcgagactt gaggtctagt tttatacgtg aacaggtcaa
     2221 tgccgccgag agtaaagcca cattttgcgt acaaattgca ggcaggtaca ttgttcgttt
     2281 gtgtctctaa tcgtatgcca aggagctgtc tgcttagtgc ccactttttc gcaaattcga
     2341 tgagactgtg cgcgactcct ttgcctcggt gcgtgtgcga cacaacaatg tgttcgatag
     2401 aggctagatc gttccatgtt gagttgagtt caatcttccc gacaagctct tggtcgatga
     2461 atgcgccata gcaagcagag tcttcatcag agtcatcatc cgagatgtaa tccttccggt
     2521 aggggctcac acttctggta gatagttcaa agccttggtc ggataggtgc acatcgaaca
     2581 cttcacgaac aatgaaatgg ttctcagcat ccaatgtttc cgccacctgc tcagggatca
     2641 ccgaaatctt catatgacgc ctaacgcctg gcacagcgga tcgcaaacct ggcgcggctt
     2701 ttggtacaaa aggcgtgaca ggtttgcgaa tccgttgctg ccacttgtta acccttttgc
     2761 cagatttggt aactataatt tatgttagag gcgaagtctt gggtaaaaac tggcctaaaa
     2821 ttgctgggga tttcaggaaa gtaaacatca ccttccggct cgatgtctat tgtagatata
     2881 tgtagtgtat ctacttgatc gatcaggctt ttgtatatct ccccaccacc tgaaacaatg
     2941 acatgatccg ttattttctt taggttggtt aaagcatctt taattgatgg aaagatcaat
     3001 acgttctcat tgtcagatgt aaaacttgaa cgtgttacga ccgcatactt tcggttgggt
     3061 aatgctccca ttgattcaaa agtcttgcgt ccaaccaaca gccattggtt ataggtaata
     3121 gctttaaaca ggagctgttc acctttggca ctccatggaa tatcagggcc attcccgata
     3181 actccattct tcgatatagc taccattagt gatagtttca caattcttcc tcagaggtta
     3241 acattttatt agtaagcatg ctcgttttgt cacccgctga tgcttaccgt taattaatat
     3301 aatcacttgt attaaataga ctttctttaa aaatacaaga cactctgtta ttacaaatcg
     3361 tgcatgccgt ctatcctgct tgcacgatgc acattatctc acccaaagaa ctcataacat
     3421 tttaatttat ttagcattaa attaaaactc taccggtaaa caagcatctc taggcgtaaa
     3481 aaacaggcac ttttttaaat cccagctaga ataggctgta taggcagaca gttgcaagac
     3541 aaggagcgtt ttatgtctaa cagtccattt ttaaattcta tacgcacgga tatgcgacaa
     3601 aaaggttatg cgctgaaaac tgaaaaaact tacctgcact ggattaagcg ttttattctg
     3661 tttcacaaaa aacgtcatcc tcagaccatg ggcagtgaag aggtcaggct gtttttatcc
     3721 agcttagcaa acagcagaca tgtagccata aacacgcaga aaatcgcttt aaatgcccta
     3781 gcttttttgt acaacaggtt tttacaacag ccgttgggcg atattgatta tatccctgca
     3841 agcaagccta gacggctacc ctctgttatc tctgcaaatg aagtgcaacg cattttgcag
     3901 gttatggata ctcgcaacca agttattttt acgctgctgt atggtgcagg tttgcgcatt
     3961 aatgaatgct tgcgtttgcg ggttaaagat tttgattttg ataatggctg catcactgtg
     4021 catgacggta agggtgggaa aagcagaaac agcctactgc ccacgcgcct aatcccagca
     4081 ataaaataac tcattgagca agcgcggctt attcagcaag acgacaactt acaaggcgta
     4141 gggccatcgc tgccttttgc tttagatcac aaataccctt ctgcttatcg acaagcggcg
     4201 tggatgtttg tctttccctc cagcacgctc tgcaaccacc cgtataacgg caaattatgc
     4261 cgccatcatc tgcatgactc cgttgcgcga aaggcattga aggcagccgt acaaaaagca
     4321 ggcatcgtta gcaagcgtgt cacttgtcat acatttcgtc actcgtttgc tacgcatcta
     4381 ttacaagcgg ggcgtgatat tcgcactgtg caagaactct tagggcataa cgatgttaag
     4441 accacgcaaa tctatacgca tgtgttgggt cagcattttg ccggcaccac cagtcctgcg
     4501 gatggactga tgctacttat caatcagtaa aagtagataa atatctttat gtagccctga
     4561 gtagcccctg atacttttta tggtgaaaaa gataaacaat catagagggg ataaatatga
     4621 gccagttcta ccattgttca gataagttta agcaagatac tgtcaatcac agtgaccatt
     4681 catggtcatt acacttcctc cagttgccag acaatgccta acataaaaac gcaaaagccc
     4741 aaaagtcaga gcttaggtgt tctaggcgtt ttctaagcgc tgaagaaata cgtcttaccg
     4801 tagattgcgt gcgtttctga agcacatttt ccttatacat ggctttatcc caggcagctt
     4861 catcaagctc ttggagtaac aagccagtaa taatgcgaca ctctcgggtt tgtagcgagc
     4921 cgctggtgag atcggagtta tagcggaaag gggtcatttg taattatttg tgggtggagg
     4981 atagttaact tattattgta gttggaagag gctgctttcc aaacattagc acctcgtaat
     5041 gcttgttact gtatattaat ttatgctctt agcgcatgtt aatgggctat tacaatgcat
     5101 aatgtccaca tcaagataac tgtggcttaa cacctgtaca agcgaaaaaa atcaatcacc
     5161 tgtccggaaa tacgggatca ctgtaaagtg ttggtgaggc agtctaggag ggggatttaa
     5221 tttgggatac tgatgaaagg attatgatca gttggatata ggatcgtact aagctgagcg
     5281 gcaggttagc tagattagtt aaccagataa gtgaaatcta gttccaaact attttgtcat
     5341 ttttaatttt cgtattagct tacgacgcta cacccagttc ccatctattt tgtcactctt
     5401 ccctaaataa tccttaaaaa ctccatttcc acccctccca gttcccaact attttgtccg
     5461 cccacaaggg ctgccga
//
LOCUS       DAJTZL010000218         5462 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.219, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000218 DAJTZL010000000
VERSION     DAJTZL010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5462
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.219"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..115)
                     /locus_tag="OO544_003749"
     CDS             complement(<1..115)
                     /locus_tag="OO544_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HCR6769248.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLE"
     gene            complement(220..1722)
                     /gene="dtpA"
                     /locus_tag="OO544_003750"
     CDS             complement(220..1722)
                     /gene="dtpA"
                     /locus_tag="OO544_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HCR6769249.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAIIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
     gene            complement(2333..2968)
                     /gene="nth"
                     /locus_tag="OO544_003751"
     CDS             complement(2333..2968)
                     /gene="nth"
                     /locus_tag="OO544_003751"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HCR6769250.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(2968..3663)
                     /gene="rsxE"
                     /locus_tag="OO544_003752"
     CDS             complement(2968..3663)
                     /gene="rsxE"
                     /locus_tag="OO544_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HCR6769251.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRTEAA
                     AERALPNGETGNV"
     gene            complement(3667..4287)
                     /gene="rsxG"
                     /locus_tag="OO544_003753"
     CDS             complement(3667..4287)
                     /gene="rsxG"
                     /locus_tag="OO544_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HCR6769252.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNTLAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(4291..5349)
                     /gene="rsxD"
                     /locus_tag="OO544_003754"
     CDS             complement(4291..5349)
                     /gene="rsxD"
                     /locus_tag="OO544_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HCR6769253.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSTLLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGLWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(5350..>5462)
                     /locus_tag="OO544_003755"
     CDS             complement(5350..>5462)
                     /locus_tag="OO544_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="electron transporter RnfC"
                     /protein_id="HCR6769254.1"
                     /translation="NAEPEEQVDPRKAAVAAAIARAQAKKAAQQKVVNED"
BASE COUNT         1445 a         1384 c         1340 g         1293 t
ORIGIN      
        1 tttcgagacg ttttttcatt aaatccacac tgacgagggt aaaatccttt ccgctctcac
       61 gcagggtgat atgggaagcg agagagcagg cacccggttt gtagaacaat ttcatcggta
      121 actccttttg tctgtggttt aaagtatggt agtgcgttgc tgaacaaaaa aaagccgcta
      181 acgaaaagtt agcggcttgt aaaagagttt ccctgaattt tacgctacag cagctttcgc
      241 cgctttgtct gcagcgtcat cctgcgtcat acggtgcagt ttaggcgcgg tcagcagcat
      301 cagtactgca ataatggcag tagcgacacc aatctgcaag aatacgcgac cataaacttc
      361 cagtgacatc agcgggtcgg taacgttatc cggcacagcc atcatacccg caacataacc
      421 accaatcagg tttgcaccgg cagtggtcag gaaccagcta cccataatga agcccatcag
      481 acgctgcgga acgagttgtg caaccattgc cagacccaga ccagagatca tcagttcccc
      541 gatgctctgc aggccatagc ttgcgaccag ccagcttaca gacacgatac cagcgtcaga
      601 cgcgaatttc gcacccagcg gcagaatcag gaacgcacca gaacacatca ccatgccgat
      661 tgcaaacttg gttggcatcg gcagggtatc gcccatcttg ttatagatag cggccagaat
      721 cggactaccg atgatgatcc agaacgggtt cagtgcctga tactgctcag gttctacggc
      781 cagacccaga atggagtgct caacgttacg aatcgcaaag aagttcagtg acgttggcat
      841 ctggctgtac agcacgaaga agataatggc ttcgagcatc aggatgaagg caacgatcat
      901 tttacgacgc gcagcacctt tcatcgcgaa ggcttcttta ccgaagataa ccacgatacc
      961 gaaggcaaca acgcccagcg ccatacgcgc aacttcctga ttgtgcagca gccaggtggc
     1021 gatagcgatc agtgccacaa caccaataat ggtcagcagc aggttacggt agttgatagg
     1081 ctcgaagtct ggttttgaac cgtactgttt aacccagcgt tggcagaagg cgaagttaac
     1141 gatagtgatc agcaggccta caacgctcaa cgcaaacgca acactccagc cgtatttcgc
     1201 ggccagccac ggcgtagcaa tcatagagaa gaaagagccg atgttgacgg acatgtagta
     1261 catggtgaat gcaccgtcca gacgcgggtc gtttttctca tagcatgtag aaagcagaga
     1321 agatgggtta gctttaaaca ggccgttacc gaccgcaata gccgccatac ccatataaac
     1381 gataccggcg tcgtgaccag accaggcaac cagagcataa ccaatcgcca gcacaatagc
     1441 accgagcata attacgcgtt tagtacccag taccttgtca cctaaccagc cgccgatagc
     1501 gaccagacca taaaccaggg cactaaagga agagaaaagg gtgattgaat ccgcttcaga
     1561 catacccagt tgtttaacca ggtaaacagc cataattcct tgtaggccgt aataaccaaa
     1621 acgttcccat aactcaatcg agaagatgag atagaacgcc ttcggttgtt tgaaagcgtt
     1681 caaactgacg ctttcagttg gtttttggtt tgcagtggac acttttacct ctttttttac
     1741 atcccatatt aacgggggtg ctttctcgcg ttatgtctaa aaaacatacg cgcaattgtt
     1801 atagtgggag gggaaacggc aggtaatgtt cactatcttg cactttcaga caagtccttt
     1861 gtaataatct gttacatata actgaggcgg ggaattcaac cattccggga aatgttatcc
     1921 agcgttaaat ttttcaaaaa acgtaatggc agattttttc actgcactga gaggggggtg
     1981 atggcttaac ggcgatatgt tctgctattt agcgttttta ctagtgagat agtccagttt
     2041 ctgaaaaata gccagtgtaa tgttttgtag gtcaatattt gttgcgtgtg taaatttaaa
     2101 ttattttaac ctgattcttg caatgttttt acattgcgac agcatcgtga tcttgatcac
     2161 gaatgaatag aaaatttcgc tattaaaaaa acaattaacc tgcatttttg gttatccgca
     2221 aacggattac ctgatgataa cgctaagcaa tggcatcatt cacgttaaaa agatgtttta
     2281 aaagaggata aagaaaggtt atcaatgggg taatcggtgt tacccctttt cttcagatgt
     2341 caactttctc tttgtattca caaagatctt caataataca agagccacag cggggcttgc
     2401 gggcaatgca ggtataacgc ccgtgcagga tcaaccaatg gtggcagtcg actttaaact
     2461 ctgccggaac cactttcagt agcttttctt ctacctgttc gacgtttttc cccggcgcaa
     2521 attgagtacg attacaaacg cggaaaatgt gcgtgtcgac agcaatagtc ggccagccga
     2581 atgcagtgtt taatacgacg ttggctgttt tacgacctac gccgggcagg gcttcaagcg
     2641 cagcacgatc ttccggaacc tcgccattat gctgctccag taagatacgg caggttttga
     2701 tgatattttc tgctttgctg ttataaagcc caatcgtttt gatataggtt ttaacccctt
     2761 caacgcccag ttcaagcatc gctgcaggcg tattcgccac cgggtaaagt ttcgccgtcg
     2821 ccttattaac actgacatcg gtcgcctgag cggaaagcag tacggcaatc agcaattcaa
     2881 aaggcgaact gaaattaagc tcggtggtgg gatgaggatt gttctcacgc aggcgagtga
     2941 ggatctccag gcgttttgct ttgttcatca gacattccct gtttcaccgt ttggcaatgc
     3001 acgttcggca gctgcttcag tacggcgctt tttcattctt tcatcaatca ggtattttcc
     3061 tgccagcatc agtcccaggc caataaatgc acctggtggc agcatcgcca gcaggaaagg
     3121 ggagtcggtg tggaaaatct ccacgcgtaa tacttttgcc cagctaccta acagcgcatc
     3181 tgcaccgtca aacaatgtgc cattgccgat aatttcgcgt agtgaaccca gcacgaacat
     3241 ggcgcaggtt gcgcccatac caattgaaaa gccgtccagt gccgaaagcg ccggaccttt
     3301 tttggcggcg aaggcttcag cgcggcccac aacgatacag ttagtgacaa tcagcggaat
     3361 aaaaatccct aatgattgat acaggccaaa ggcgtaggcg ttgatcagca tctgtacagc
     3421 gctgaccacc gaggcgatga tcatcacgta aatgggaatg cggatctcgg ctggcgtcca
     3481 gtgacgcagc gtcgaaatgg tcaggttggt cagcgtcagt accagcgtag tcgcaagtcc
     3541 taaacccaga gcgttagtgg cagtggacgt gaccgccaac agaggacaaa ggccgagcaa
     3601 ctggaccagc gcagagttgt ttttccacaa cccctgaaca ataacgtctt taatttcgct
     3661 cacgctttat tctccacagg caggaagttg agaaagttgt gccggtaacg tctgagcgta
     3721 caatccggcg cgttttaccg cattaaccac cgcgcgggga gtaatcgtcg cgccggtgaa
     3781 ctggtcgaaa tcaccaccat ctttcttcac cgcccagtgc gcatcatccg caccactgat
     3841 ttttttaccc gcaaaatggg tgatccagtc agaaaggcgc agttcgattt tatcgccaag
     3901 ccctggcgtt tcgtggtgct ctgtcacgcg cgtgccaagt accgtgccgt taaaatcggc
     3961 tcccaccagc agctgaatcg caccggaata gccatctggc gcggttgctt ccagaacggc
     4021 ggctaccggt ttgtcatcct gtttggcgat gtaaacccga tgctcacctt tacctaactc
     4081 tggcgcagtt accagatagc aactctgtgc cagcgtattg ttatagcgtt cggctggcag
     4141 cacctgatca aataatgcct tttgttgcag actggcctgt tcagcaatcg tcgttttggt
     4201 catctggttg atggccgcag ttaaccctgt tgaacccgct gcaaatagcg ctaacgtaat
     4261 gccgtgtttt cggatagttt tcagcatggt ttaccctttg cgatggccgt agacgcgcgg
     4321 acgcgtgtag taatcgatca gaggaaccgt gatgttcgcc agcaggacgg caaaagccac
     4381 gccgtcagga tagccgccga aactgcggat caaccagact aataagcccg caagcgcgcc
     4441 gaaaataaga cgaccacgat tggtcgtaga agcggtaacc gggtcagtca aaataaagaa
     4501 tgcgccgagc atggtcgcac cagacagcag atgaatttgc ggtgctgcca gtgtttctgg
     4561 tgagaacaac cagcccaacg ttgcgcataa cgccagcgtt actaagaagc tgaggggaat
     4621 atgccagcga atcgctttct gccatagcaa ccacaggccg ccagccagcc aggcgagatt
     4681 tacccattgc caaccagcgc ccgccagaat accgctgtag atcggatatt gcataatctg
     4741 ttcaaccgaa tgaccggcac ggacagaggt tttaaatgta tccagcggtg tcgcctgact
     4801 aatgccatca atacctaagc gtagtgtgtt catatcacca ccactggcgg tatgcccgct
     4861 gaaaataacc tgtatggcgt cgataaaacc agggatgttg accgcaattt catgtggcgg
     4921 taaccagctg gtcatctgca ccgggaagga gatcagtaag accacataac caatcattgc
     4981 cggattaaac gggttttgcc ccagaccgcc atacaactgt ttagcgatga tcaccgcaaa
     5041 caccgtaccc agcacgacca tccaccatgg cgcgaggggg ggaatactta ccgccagcaa
     5101 taagcctgtc agcaatgctg agttatcttt caacgttgcg gctaccgact gcttgcgtag
     5161 tttgagtacg agagcttcgg ctaacagagt actaaccgat gccaacagga tctgaacgag
     5221 agtaccccaa ccaaaaaacc acagttgcgc tgcgattcct ggcacggctg cgagcaacac
     5281 caacagcata atgcgcgatg tctggcgctg gttatgggta taaggggagc tagctattct
     5341 gaataccatt tagtcctcgt ttacaacctt ctgctgggcg gcttttttgg cctgagcgcg
     5401 ggcaatagcc gcggcaactg ccgctttgcg cggatcaacc tgttcttctg gttctgcgtt
     5461 ag
//
LOCUS       DAJTZL010000219         5461 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.220, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000219 DAJTZL010000000
VERSION     DAJTZL010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5461
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.220"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..293
                     /locus_tag="OO544_003756"
     CDS             <1..293
                     /locus_tag="OO544_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein I (serine
                     chemoreceptor protein)"
                     /protein_id="HCR6769255.1"
                     /translation="ASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEE
                     QASRLTQAVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRIAEQDPNWETF"
     gene            339..1940
                     /gene="tap"
                     /locus_tag="OO544_003757"
     CDS             339..1940
                     /gene="tap"
                     /locus_tag="OO544_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HCR6769256.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNQVE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISTLVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSQEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMIDIVSSVTRVND
                     IMGEIASASEEQQRGIEQVAQAVSQMDQVTQQNASLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            1959..2819
                     /gene="cheR"
                     /locus_tag="OO544_003758"
     CDS             1959..2819
                     /gene="cheR"
                     /locus_tag="OO544_003758"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HCR6769257.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHAHRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQNTQQEILRRFVPLLKPDGLLFAGYSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            2822..3871
                     /gene="cheB"
                     /locus_tag="OO544_003759"
     CDS             2822..3871
                     /gene="cheB"
                     /locus_tag="OO544_003759"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HCR6769258.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLAKISAGQAIRI"
     gene            3886..4275
                     /gene="cheY"
                     /locus_tag="OO544_003760"
     CDS             3886..4275
                     /gene="cheY"
                     /locus_tag="OO544_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HCR6769259.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            4286..4930
                     /gene="cheZ"
                     /locus_tag="OO544_003761"
     CDS             4286..4930
                     /gene="cheZ"
                     /locus_tag="OO544_003761"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HCR6769260.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGLVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKVGVVASQDQVDDLLDSLGF"
     gene            5132..5341
                     /locus_tag="OO544_003762"
                     /pseudo
     CDS             5132..5341
                     /locus_tag="OO544_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310617.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
BASE COUNT         1314 a         1327 c         1585 g         1235 t
ORIGIN      
        1 ttgcatcggc atcggatgaa cagagccgtg gcatcgatca agtcgcattg gcggtttcgg
       61 aaatggatcg cgtcacgcaa cagaacgcat cgctggtgca ggaatcagct gccgccgccg
      121 ctgcgctgga agaacaggcg agtcgtttaa cgcaagcggt ttccgcgttc cgtctggcag
      181 ccagcccact caccaataaa ccgcaaacac catcccgtcc tgccagtgag caaccaccgg
      241 cacagccacg actgcgaatt gctgaacaag atccaaactg ggaaacattt tgacgagtat
      301 ttactaacgc ggtcattgcc gcctgatggg gagcgttgat gtttaatcgt attcgaattt
      361 cgaccacgct gtttttaatt ttgattctct gcgggatctt gcagattggc agtaacggca
      421 tgtctttttg ggcatttcgc gacgatttgc aacgactgaa tcaggtcgag cagagcaatc
      481 agcaacgtgc ggcattagcg caaactcggg cggtaatgtt acaggccagt accgcgctga
      541 acaaagcggg cactctgacg gcacttagct atccggcgga tgacattaaa acgttgatga
      601 cgacggcgcg cgccagtctg acgcaatcca ccacgctgtt taaaagtttt atggcgatga
      661 ctgcgggcaa cgagcacgtc agggcattgc aaaaagagac ggagaaaagt tttgcccgct
      721 ggcacaacga tctcgaacat caggcgacct ggcttgaaag taatcaactt tcggatttcc
      781 tcactgcgcc ggtgcaggga tcacagaatg cgtttgacgt taactttgag gcctggcagc
      841 tggagatcaa ccatgtgctg gaagccgcca gtgcgcaaag ccagcgtaac tatcagattt
      901 cgacactggt gtttatcagc atgattattg ttgcggcgat ctacatcagc agtgcgctgt
      961 ggtggacgcg caagatgatt gttcaaccac tggccattat cggtagccat tttgacagca
     1021 ttgctgcggg taatctggcg cgtccgattg cggtatatgg tcgtaatgag atcaccgcca
     1081 tttttgccag tctgaagacc atgcagcagg ctttgcgtgg gacggtaagt gatgtgcgta
     1141 agggaagcca ggagatgcac attggtatcg cggagattgt cgcaggcaat aacgatctct
     1201 caagtcgtac cgaacagcag gcggcatcgc tggcacaaac ggccgccagt atggagcaat
     1261 taaccgccac ggtagggcaa aacgccgata acgcacgaca ggcgtcggaa ctggcaaaaa
     1321 atgccgcgac aacggcgcag gcgggcggtg ttcaggtcag taccatgact cacaccatgc
     1381 aggagatcgc caccagctcg caaaaaattg gcgacattat cagcgttatc gacggaattg
     1441 ctttccagac caatattctg gccctgaatg cggcagtgga agcggctcgc gccggagagc
     1501 aggggcgtgg ttttgcggta gtggcaggtg aagtgcgcaa tcttgccagc cgtagcgcgc
     1561 aggcagcaaa agagatcaaa gggctgatcg aagagtcagt caatcgtgtc cagcagggtt
     1621 cgaaactggt gaataacgcc gccgcgacca tgatcgatat tgtcagttcg gtgacccgcg
     1681 tgaacgacat tatgggagaa attgcctctg cgtcggaaga acaacagcgg gggattgagc
     1741 aggttgcaca ggctgtcagc cagatggatc aggtgactca gcagaacgcc tcgctggtag
     1801 aagaagcggc ggtggcaacg gaacaactgg cgaatcaggc cgaccatctt tcgtcgcgtg
     1861 tggcggtatt tacccttgaa gaacatgaag tagcacgaca tgagtcggcg cagttacaaa
     1921 ttgcgccagt ggtatcctga agtgattgag aaggcgctat gacttcatct ctgccctgtg
     1981 ggcaaacgtc tttattgtta cagatgaccg agcgcctggc gctttccgac gcgcattttc
     2041 ggcggataag tcaattgatc tatcaacgag ccgggatcgt tctggctgac cataaacgcg
     2101 acatggttta caaccgactg gttcgtcgtt tgcgttcgct gggactgacg gatttcggtc
     2161 attatctgaa cttgctggaa tctaatcagc acagcggtga gtggcaggcg tttatcaatt
     2221 cgctgaccac gaatctgacg gcatttttcc gtgaggcaca ccatttccct ctgctcgcgg
     2281 atcacgcaca tcgccgttct ggcgagtatc gcgtatggag cgcggcggct tcgaccggcg
     2341 aagagccgta cagcattgcg atgacgctgg ctgacacatt gggcaccgcg cccggacgct
     2401 ggaaagtgtt tgccagtgat atcgacaccg aagtgctgga aaaagccaga agcggtattt
     2461 atcgccatga agagttgaaa aacctgacgc cgcagcaact gcaacggtat ttcatgcgag
     2521 ggacgggacc gcatgaaggg ctggtacgcg tgcgtcagga gctggcgaac tatgttgatt
     2581 ttgccccgct gaatctactg gcgaaacagt acaccgtgcc ggggccgttt gatgcgatct
     2641 tctgtcgtaa cgtcatgatc tacttcgatc aaaataccca gcaggagatt ttgcgccgct
     2701 ttgttccgct ccttaaaccc gacggattgc tgtttgcggg ttactctgaa aactttagcc
     2761 accttgagcg ccgcttcacg ctgcgtggtc agacggtgta tgcgctaagt aaggattaac
     2821 gatgagcaaa atcagggtgt tatctgtcga tgattcggca ctgatgcgcc agatcatgac
     2881 agaaatcatc aacagccata gcgacatgga aatggtggcg accgcgcctg atccgctggt
     2941 cgcgcgtgac ttgattaaga aattcaatcc cgatgtgctg acgctggatg ttgaaatgcc
     3001 gcggatggac ggactggatt tcctcgaaaa attaatgcgt ttgcgtccaa tgcccgttgt
     3061 gatggtttct tccttgaccg gcaaaggatc agaagtcacg ctgcgcgcgc tggagctggg
     3121 ggcaatagat tttgtcacca aaccgcaact gggtattcgc gaaggaatgc tggcgtatag
     3181 cgaaatgatt gctgaaaagg tgcgtacggc agcaaaggcg agccttgcag cacataagcc
     3241 attgtcagca ccgacaacgc tgaaggcggg tccgttgttg agttctgaaa aactgattgc
     3301 gattggtgct tcaacgggtg gaactgaggc aattcgtcac gtactgcaac cgttgccgct
     3361 ttccagcccg gcattgttaa ttacccaaca tatgccgccc ggtttcaccc gctcttttgc
     3421 cgacagactt aataagcttt gccagatcgg ggttaaagaa gccgaagacg gagaacgtgt
     3481 cttgccgggg catgcctata ttgcgccggg cgatcggcat atggaactgg cgcgtagtgg
     3541 cgcaaattat caaatcaaaa ttcacgacgg cccggcggtt aaccgtcatc ggccttcggt
     3601 agatgtgttg ttccattctg tcgccaaaca ggcggggcgt aatgcggttg gggtgatcct
     3661 gaccggtatg ggcaacgacg gcgcggcggg aatgttggcg atgcgtcagg cgggggcatg
     3721 gacccttgcg caaaacgaag caagttgcgt ggtgttcggc atgccgcgcg aggccatcaa
     3781 tatgggtggt gtctgcgaag tggtcgatct tagccaggta agccagcaaa tgttggcaaa
     3841 aattagtgcc ggacaggcga tacgtattta aatcaggagt gtgaaatggc ggataaagaa
     3901 cttaaatttt tggttgtgga tgacttttcc accatgcgac gcatagtgcg taacctgctg
     3961 aaagagctgg gattcaataa tgttgaggaa gcggaagatg gcgtcgacgc tctcaataag
     4021 ttgcaggcag gcggttatgg atttgttatc tccgactgga acatgcccaa tatggatggc
     4081 ctggaattgc tgaaaacaat tcgtgcggat ggcgcgatgt cggcattgcc agtgttaatg
     4141 gtgactgcag aagcgaagaa agagaacatc attgctgcgg cgcaagcggg ggccagtggc
     4201 tatgtggtga agccatttac cgccgcgacg ctggaggaaa aactcaacaa aatctttgag
     4261 aaactgggca tgtgaggatg cgactatgat gcaaccatca atcaaacctg ctgacgagca
     4321 ttcagctggc gatatcattg cgcgcatcgg cagcctgacg cgtatgctgc gcgacagttt
     4381 gcgggaactg gggctggatc aggccattgc cgaagcggcg gaagccatcc ccgatgcgcg
     4441 cgatcgtttg tactatgttg tgcagatgac cgcccaggct gcggagcggg cgctgaacag
     4501 tgttgaggcg tcacaaccgc atcaggatca aatggagaaa tcagcaaaag cgttaaccca
     4561 acgttgggat gactggtttg ccgatccgat tgaccttgcc gatgcccgtg aactggtaac
     4621 agatacacga caatttctgg cagatgtacc cgcgcatacc agctttacta acgcgcaact
     4681 gctggaaatc atgatggcgc aggattttca ggatctcacc gggctggtca ttaagcggat
     4741 gatggatgtc attcaggaga tcgaacgcca gttgctgatg gtgctgttgg aaaacatccc
     4801 ggaacaggag tcgcgtccaa aacgtgaaaa ccagagtttg cttaatggac ctcaggtcga
     4861 taccagcaaa gtcggcgtgg tagccagtca ggatcaggtg gacgatttgt tggatagtct
     4921 tggattttga tttgtattgc ctgatgtggc gtgaccacgt catatcaggc ggtctgataa
     4981 ggcgatgacg ccgcatccga caaacgcaca ctatgcttga tgtgtggcag caaaagccct
     5041 aaatcccgcc tgttttgccc cttactcaaa ccattgaacg ctttgcgctc tggcatcatt
     5101 taagcttaat actctttcca ggattggcga cgtgtctgac gagagcgacg acaaaacaga
     5161 agcccccaca cctcaccgac tagaaaaagc gcgggaagag gggcaaatcc cgcgttcccg
     5221 tgaactgacc tcgttgctga ttttgttagt gggcgtgtgt gttatctggt ttggcggtgt
     5281 gtcgctggcc cgtcgattgt cgggcatgct ctccgctggg ctgcattttg atcacagtat
     5341 ttatatgaac gcatcccgtt tgcaaggcat ccattgtttt gacatgacgg gtaggttgca
     5401 gctttatatc cggctttatt gcagccgtaa cgctgcgagc ccctatgtaa ttcgccgact
     5461 c
//
LOCUS       DAJTZL010000220         5281 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.221, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000220 DAJTZL010000000
VERSION     DAJTZL010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5281
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.221"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..471)
                     /locus_tag="OO544_003763"
     CDS             complement(<1..471)
                     /locus_tag="OO544_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR6769261.1"
                     /translation="MKHIQIRNSDMAWHIAANIQFPPNFDESKQYPAIISVHPFGSCK
                     EQTSGNIYGKALAEKGYLVLAYDASFQGESGGEPRWIEDPTQRVEDISRVIDYAVTLP
                     YVNAERIGVLGVCGGGGYAINAALTEKRIKAVVSITGVNIGRLFREGFSNYDPIG"
     gene            complement(471..1391)
                     /locus_tag="OO544_003764"
     CDS             complement(471..1391)
                     /locus_tag="OO544_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR6769262.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYQAIVVSHPGG
                     GVKEQTAGTYAKKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDISAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPYVEAGSNARTSDISSGEYAVMPLAPMKESDAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITFDAYHMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            1529..2428
                     /gene="pgrR"
                     /locus_tag="OO544_003765"
     CDS             1529..2428
                     /gene="pgrR"
                     /locus_tag="OO544_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HCR6769263.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSALASLSELQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSVPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
     gene            2765..4378
                     /gene="mppA"
                     /locus_tag="OO544_003766"
     CDS             2765..4378
                     /gene="mppA"
                     /locus_tag="OO544_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HCR6769264.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKDRV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(4429..>5281)
                     /gene="ynaI"
                     /locus_tag="OO544_003767"
     CDS             complement(4429..>5281)
                     /gene="ynaI"
                     /locus_tag="OO544_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HCR6769265.1"
                     /translation="INYVIENYKLKFITPDVIDFICTSLIAVILTIKLFLLINQFEKQ
                     QIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAGKD
                     ILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPNSL
                     FSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLVYF
                     NQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMDNI
                     TPPEQGR"
BASE COUNT         1429 a         1203 c         1226 g         1423 t
ORIGIN      
        1 gccaatcgga tcgtaattgc tgaaaccttc ccgaaaaaga cgcccgatat taacacccgt
       61 aatactcact actgctttaa ttcgtttttc tgtcagcgcg gcattaatgg catagccacc
      121 acccccacac actcccagca cgccaatacg ttcagcatta acatatggca gagttactgc
      181 ataatcgata acacggctaa tatcctctac gcgctgtgtc ggatcttcta tccagcgagg
      241 ctccccacca gattctccct ggaaactggc gtcataagcg agaacgagat agcctttttc
      301 agccaatgcc ttgccataga tattgccaga cgtctgctcc ttacaactgc caaaaggatg
      361 gacgctgata atcgccggat actgttttga ctcatcaaaa tttggtggaa actgaatatt
      421 ggcagcaata tgccaggcca tatcactatt gcggatttga atatgtttca ttacagagtt
      481 tcctcaaaga acggagctaa tactgaaatt gcttcagcca cataggcttt gccgtcataa
      541 agatccatat ggtttgcgcc ttcaacaatg tgatagcgtt tatcttggct tgatgctcga
      601 tcgtacaggt cgtcactcat ccatttgctc cctgcctggc tgcccgccac aatctgcatc
      661 ggctgagtca ggtacacttc cgccatatgg taagcatcaa aggtaataat ctggttaagg
      721 ctgcgcaaag tagcgtaacc cggtgctgtt ggatactgcg cgcgaggggt gtggtaatac
      781 tcccaggcct gacgcagttc ttcgttcggc gcatcggact ccttcattgg tgccagtggc
      841 ataacggcat attctccgct gctaatatcg ctggttcggg cgtttgaacc cgcttcaacg
      901 tatggcaggg catcaataga tttcacattg ttttcccaac cattacggaa catcgaacca
      961 atattgaccg cactaacggt accgatggcc ttgatgcggc gatcctgaat tgcagcattg
     1021 gctgtatatc ctgcaccggc acaaattccc atcgcaccaa ttcgggtatt gtcgacataa
     1081 gaaagcgttg tcaggtaatc aatcacggca ctgatgtctt cagtacgaat gtatgggttt
     1141 tctaactgac gcggctcgcc gccactttca ccttgataag atgcgtcata agcaatagtg
     1201 acaaatccct tttccgccag ttttttggca taggttccgg ccgtttgttc tttaacgccc
     1261 ccacctgggt gagataccac aattgcctga tactgacggg tttcatcaaa ttttggaggg
     1321 aaatagatca ctgcagacaa agagatggtt ggattattgc tgttagtgaa gctgacttta
     1381 ttattcatca ttccgttcct catggagttg tcggttcgtt ttaacggttg gtgatatcac
     1441 tatagatatt gatcattaag ttgattagac ccaaaattat gattagacct atcatttaaa
     1501 tgattaatag attgcatgga gatactgaat gaagcgtgaa gaaatcgctg atctgatggc
     1561 gtttgtcgtc gttgcagagg agcgtagctt cactcgtgca gcagcccgcc tgagcatggc
     1621 gcagtcagct ttaagccaga tagtgcgtcg tatagaagaa cgattgggat tgcggcttct
     1681 gacgcgaacc acgcgcagcg ttgttccaac tgaagcgggc gagcatcttt tgtctgttct
     1741 tggccctatg ttgcatgaca tagattcagc cctggcatcc ctgagcgagc tgcagaaccg
     1801 cccatccggg acaatacgta ttactactgt agaacatgca gccaaaacga tattgttacc
     1861 agcaatgcgc acattcctga aatcgcatcc tgaaattgat attcagctca ccattgatta
     1921 tggtttgacc gatgtcgttt ctgaacgttt tgatgcaggc gtccgtctgg gtggggagat
     1981 ggataaagat atgatcgcca ttcgaatcgg gccagatata ccaatggcta ttgttggctc
     2041 accggattat ttttctcgcc gaagtgttcc aacgtcagtg tcacaattaa tagatcatca
     2101 ggcaattaat ttgtatcttc ccacatcggg tacagcaaat cgctggagat taatacgcgg
     2161 tggacgtgaa gttcgtgttc gcatggaagg tcagctttta ctgaatacga tagacctgat
     2221 cattgatgct gcaattgatg ggcatggatt ggcgtatcta ccttatgatc aggttgagcg
     2281 ggctattaaa gaaaaaaaac tgatacgtgt tttggataaa ttcacaccag atttacccgg
     2341 ttatcacctg tactatccac accgtcgaca tgctggctcg gcattctcat tatttataga
     2401 taggctgaag tataaaggtg ctgtttagca ctacttgctg atacattaat ttaatttttc
     2461 tcttaacgta ttctcagttc ctttcaacgt tttggttatt ttttattctt cgtacaatgg
     2521 cgacagatgc tgattatgat accgaaaact ggtttgaacg tgcgaagcct gaacgtagtg
     2581 ttcggtgttc tatgtgcttt accgcatttt gtcgtctatt atttacacta aatctgattt
     2641 aatatattga tacataaaac attttatgct ccctgtgtca cttttttgat ggaagttgtt
     2701 tgcatttctt taaggcgaaa caaataatta cgcatcaatt ttaatgtcgg ttagagggaa
     2761 acttatgaag cactctgttt cagtcacgtg ttgtgcgctg ttggtcagca gcatttctct
     2821 ttcgtatgct gcagaagttc cgagcggcac agtactggca gagaagcagg agctggtgcg
     2881 ccacattaaa gatgagcctg cgtcgctgga tcccgctaaa gccgtgggcc tgccagagat
     2941 tcaggtcatt cgcgatctgt ttgaaggact ggtgaatcag aacgagaaag gggagattgt
     3001 ccccggcgtt gcgactcagt ggaaaagtaa tgacaaccgt atctggactt ttaccctgcg
     3061 cgataacgca aaatgggcgg atggcacacc ggtaacggcg caagattttg tctacagctg
     3121 gcagcgtctg gtggacccga aaacgctatc gccatttgca tggtttgccg cgctggcggg
     3181 aatcaacaac gcacaggcga ttattgatgg taaagctacg ccagaccagc ttggcgtcac
     3241 cgcagttgat gcccatactt tgaaaattca gcttgataaa ccgttgccgt ggtttgtgaa
     3301 tttaaccgct aactttgcct tcttcccggt gcaaaaagcc aacgtagaaa gcggtaaaga
     3361 gtggacgaaa cccggaaatc tgatcggcaa tggcgcttat gttcttaaag atcgcgtagt
     3421 caatgaaaaa ctggtcgtgg taccgaatac ccattattgg gataacgcca aaacggtact
     3481 gcaaaaagtg accttcctgc caattaatca ggaatccgca gccactaagc gttacctcgc
     3541 gggggatatt gatatcaccg aatccttccc gaaaaatatg tatcagaagc tgttgaagga
     3601 tattccgggg caggtttata cgccgccgca gctcgggacc tattattatg cgtttaacac
     3661 gcaaaaaggg ccgacggcag atcagcgcgt tcgtctggca ttaagtatga cgatagatcg
     3721 ccgcctgatg accgaaaaag tattagggac gggcgaaaag ccagcgtggc attttacacc
     3781 agatgttacc gccggattta cgccggaacc ttcgccgttt gaacaaatga gtcaggaaga
     3841 actgaatgcg caggcaaaaa ctttgttgag cgcagctggt tatggtccgc aaaaaccgct
     3901 gaagctgacg cttttgtata acacttcaga aaaccatcaa aaaattgcga ttgctgtagc
     3961 atcgatgtgg aaaaagaacc ttggcgtaga tgttaaattg caaaatcagg aatggaaaac
     4021 ctatatcgat agccgtaaca ccggcaattt tgatgttatc cgcgcctcat gggtggggga
     4081 ttataatgaa ccctccactt tcctgacatt attaacgtca acgcattcag gaaatatttc
     4141 acgctttaac aatccggcat acgacaaagt tctggcccag gcatcgacgg aaaataccgt
     4201 taaagcgcgt aatgccgatt acaacgcggc agaaaaaatc ctcatggagc aagcaccgat
     4261 tgcaccaatt tatcaatata ccaatggacg attaatcaag ccgtggctga aaggttatcc
     4321 cattaataac ccggaagatg tggcgtacag ccggactatg tatattgtga agcattgatg
     4381 tgatgggaac tggcgttacc cttgtggata acgccagtga tgtctgattt agcgcccctg
     4441 ttccggcggt gtaatgttat ccatatacag cgtctggctg gggaaggcaa agtccgcgcc
     4501 gtgtgactgc acaatatcga taatcttcaa ataaacgtct tgctgtgcag caagccattc
     4561 agcccatacc gtggttttgg taaagcaata aaccataata ttcaatgacg agtcggcaaa
     4621 ctggttgaaa taaaccagta aggtttgtcg ctggtcgatg gccgggtgat ttttcagcat
     4681 ctcacgtacg gcttcgacaa taacgcccac ttttgccgca tcctcataac gtaaaccaat
     4741 ggtcgtggta atgcggcggt tggtcattcg tcctgggttt tctacgctga tcgacgaaaa
     4801 cagcgagttc ggtacgtaca atggacgatt atcaaaggtc gtaattttgg taattcgcca
     4861 gccaatttcc gctactgtac cttcgatatt tctgtccggt gaacggatcc agtcgccaat
     4921 actgaaagga cggtcgaaat agagcatgat cccggaaaag aaattactca gaatatcttt
     4981 accggccata ccgacagcca gaccaccaat accaccaaag gtcagcaagc cagaaaggct
     5041 catgccgaaa tgttcgccat aaagcagaac aagcaccaca ataatggtga ttttgataat
     5101 gcgcgacata atccgcgcac tggtgatatc gcgacctttt ttaatctgct gtttttcaaa
     5161 ctgattaatc agcaaaaata gcttaatcgt cagaataacc gcaatcaggg acgtacagat
     5221 aaaatcgata acgtctgggg tgataaattt gagtttatag ttttctataa cataattaat
     5281 a
//
LOCUS       DAJTZL010000221         5219 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.222, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000221 DAJTZL010000000
VERSION     DAJTZL010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5219
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.222"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(291..575)
                     /locus_tag="OO544_003768"
     CDS             complement(291..575)
                     /locus_tag="OO544_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6769266.1"
                     /translation="MISQNVWAPPFFFGPWVELENHTGDIAYTVYFDTLSNVPGPFIA
                     FIWYAPNGPRTVNVIGPASYQISCKGAGVDKIKFKSVSGTGQIIHVYTTL"
     gene            complement(593..790)
                     /locus_tag="OO544_003769"
     CDS             complement(593..790)
                     /locus_tag="OO544_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6769267.1"
                     /translation="MKEEKVLLHRFLFVVRNKNGCELSCSADLMGTRDDVYKYFSDSV
                     SGLDVELIDVSCESEWEEHSH"
     gene            1002..1298
                     /locus_tag="OO544_003770"
     CDS             1002..1298
                     /locus_tag="OO544_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001679964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6769268.1"
                     /translation="MSKTKSGRHRLSKTDKRLLAALIAAGYEERTARDLIQKHVYTLT
                     QADLRHLVSEISNGVGQSQAYDAIYQARRIRLARKFLNGKKPEEGLNAWKGDLP"
     gene            complement(1843..2076)
                     /locus_tag="OO544_003771"
     CDS             complement(1843..2076)
                     /locus_tag="OO544_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HCR6769269.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDN
                     AMLNERVMQLSQQVQHLSEQTERLSQLYSENRR"
     gene            complement(2083..2613)
                     /locus_tag="OO544_003772"
     CDS             complement(2083..2613)
                     /locus_tag="OO544_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HCR6769270.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTETMLNDEFRKLEKSVSKAVTSN
                     ETKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVIFLFTV
                     SGGTLWYLGGMIQANLEEIRKQNENLEKLNAKTWGVRYHEGSNGRFLVLPEGMKAETN
                     WTMENGKQNAVRLVQE"
     gene            complement(2591..3349)
                     /locus_tag="OO544_003773"
                     /pseudo
     CDS             complement(2591..3349)
                     /locus_tag="OO544_003773"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3291..3614)
                     /locus_tag="OO544_003774"
     CDS             complement(3291..3614)
                     /locus_tag="OO544_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HCR6769271.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGRLPT
                     LAPPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQG
                     TRDDS"
     gene            3679..3870
                     /locus_tag="OO544_003775"
     CDS             3679..3870
                     /locus_tag="OO544_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HCR6769272.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDH
                     ADELYRSCLARFGDDGENL"
BASE COUNT         1367 a         1434 c         1118 g         1300 t
ORIGIN      
        1 aaaaaaacaa aggcgtcata atagaagtaa acacagatag tttatgtaag aaaaaactaa
       61 acgaaataac tagtaaaaat atagggaaac ccctgatttc atattttgat ggaaaagtaa
      121 tatcccaacc tgcaatcatt cattctgcac taaatccaac ccattattat caacctgtga
      181 acgatgaaac aacagtgatg gaaatcataa aagcatattc aaaataatat taatagatat
      241 aatcctgctt aaggtatagc ttaggcagga ttatatctat tcagctctta ttataaggta
      301 gtgtatacat gaataatctg ccctgtccca ctgacacttt taaatttaat tttatcaaca
      361 ccagcgccct tgcaagagat ctgataagat gctggaccaa taacgttaac tgttcttggc
      421 ccattagggg cataccagat aaaagcaata aatggaccgg gtacattaga caatgtatca
      481 aaatagacag tgtacgcgat atcaccagta tgattttcaa gttcaaccca aggaccaaaa
      541 aagaaaggag gagcccaaac attttgagaa atcatataat ctcctgatta gttcaatgcg
      601 aatgttcttc ccattcactc tcgcaagaaa catctataag ctcaacatct agcccactaa
      661 cagaatcaga gaaatactta taaacatcat cccttgttcc cataaggtct gccgaacatg
      721 atagttcaca accattctta ttcctaacta caaataagaa acggtgaagc aatacttttt
      781 cttctttcat tttatccctc cctttattta cacagaatat actgcatata taaacactag
      841 ttttatatac agtataaatt acgccgcatt atttttcctg tcaatgatat gaattttcat
      901 ttttgtgttc tgaatcaccc ttataaaaat caggaaggga agattcgcag cagaaaaaca
      961 gcaccggata acatcaggag aaaaaacaga gaggagataa cgtgagcaaa acaaaatctg
     1021 gtcgccaccg actgagcaaa acagacaaac gcctgctggc tgcacttatc gctgccggat
     1081 acgaagagcg gacagcccgt gacctcatcc agaaacacgt ttacacactg acacaggccg
     1141 acctgcgcca tctggtcagt gaaatcagta acggtgtggg acagtcacag gcctacgatg
     1201 cgatttacca ggcaagacgc attcgtctcg cccgtaaatt cctgaacgga aaaaaaccgg
     1261 aagaagggct gaacgcctgg aagggtgatt taccataatc acttattgta cgcaccgctg
     1321 aaacgcgttc ggcgcgatca cggcagcaga caggtaaaaa tggcaacaaa ccacccgaaa
     1381 aactgccgcg atcgcacccg gtaaatttta accacatgca tagctatgca gccatgtgaa
     1441 tcatgcagga attgccagcc agagacagct gaaacggatt tttttcatgc tttcggaagc
     1501 gaagaagacg gggaccggag cgggaaaaca gaagttcagg ggaatgagcg cgatctggca
     1561 atagaggcaa aacacagcaa caaaagacac accagaatcg cgcccgtatg cgttttaacg
     1621 cgttcctgtg ttttcagcaa ggtctgactg aagcgcgttc ccggtgcccc cgaaccaacc
     1681 attatcccaa aaaatgcacc ggaaacgcgc tgacaacatt ttagcctgtc tctgattacc
     1741 atcccagcga agggccatcc agtgtccgtt cctgtatttc cagctggcgc tcacgctcca
     1801 gggataacgc atgttcgcgt tcctgtatca gccgttcgtc ccttatctcc tgttctcgct
     1861 gtataactgg ctcaagcgtt ctgtctgctc gctcaagtgc tgcacctgct gactcaactg
     1921 catgacccgc tcgttcagca tcgcgttgtc ccgttgcgta agcgaaaaca ttttctgcaa
     1981 ttccacgaag gcgctctccc attcgttcag ccgctgcata tagtcctgtt gcagttgctc
     2041 taatgcgttc agcaaatgtc tttccagctc cgtcatgctc tgttactcct gtaccagcct
     2101 tacggcattc tgtttcccgt tttccatcgt ccagttcgtt tcggctttca tcccctcggg
     2161 caacaccaga aaacgtccgt tgctcccctc gtgataacgc acgccccatg tcttcgcgtt
     2221 cagcttctcc agattctcgt tctgcttcct gatttcttcc aggttcgcct gtatcatccc
     2281 gccaagatac cagagcgtcc cgccactcac ggtaaacagg aaaatgacca tccccagtaa
     2341 catcatgccc gtattccctg ccagctttaa cacgtccttc ctgtgctgca tcatcgcctc
     2401 tttcacccct tcccggtgtt ttttcagtga ttcctctgtc gaagctgtga acagggctat
     2461 agcgtctttg attttcgtct cgtttgatgt cacagccttg cttacagatt tttcgagctt
     2521 cctgaactcg tcgttcagca ttgtctctgt agattcggct ctctctttca gctttttctc
     2581 gaactccgcg cccgtctgta aaagattgct catagaatgc tcctttcagt ctgatattct
     2641 tcccgccgtt cgggtctgca atgctgagac tgcttcgcgt caccctgacc acttcaagcc
     2701 ctgcctctgt gagcgcctga atcacatcct gacggccttt tatctccccg acatgataaa
     2761 gagcgtctat cccgcgtgtg acgctctctg caagcgcctg tttcgtttcc ggcaggttat
     2821 cagggagagt cagtatccgg cggttctccg gggcgttggg gtcatgcagc ccgtaatggt
     2881 gattcaccag cgtctgccag gcatcaattc tcggcctgtc tgcccggtcg tagtacggct
     2941 ggaggcgttt tccggtctgt agctccatgt tcggaatgac aaaattcagc tcaagccgtc
     3001 ccttgtcctg gtgctccacc cacaggatgc tgtactgatt tttttcgaga ccgggcatca
     3061 gtacacgctc aaagctcgcc atcacttttt cacgtcctcc cggtggcagc tccttctccg
     3121 cgaacgacag cacaccggac gtgtatttct tcgcaaatgg cgtggcatcg atgagttccc
     3181 gtacttcttc cggattcccc tgaagcaccg ttgccccttc ccggttacgc tcccttccca
     3241 gcaggtaatc aaccggacca ctgccaccac cttttccccg ggcatgaaac ttaactatca
     3301 tcccgcgtcc cctgttccct gacggccaga cgcagccggc gcagctcatc cccgatggcc
     3361 atcagtgcgg ccaccacctg aacccggtca ccggaagacc actgcccgct gttcaccttc
     3421 ctcgctgtct gattcaggtt attcccgatg gcggccagct gacgcagtaa cggcggtgcc
     3481 agtgtcggca gcctcccgga acgggcaacc ggctcaccca agcagacccg ccgcatccat
     3541 accgccagct gtttaccctc acagcgttcc agtaaccggg catgttcatc atcagtaacc
     3601 cgtatcgtga gcatcctctc gcgtttcatc ggtatcatta cccccatgaa cagaaatccc
     3661 ccttacacgg aggcatcagt gaccaaacag gaaaaaaccg cccttaacat ggcccgcttt
     3721 atcagaagcc agacattaac gctgctggag aagctcaacg aactggacgc ggatgaacag
     3781 gcagatatct gtgaatcgct tcacgaccac gccgatgagc tttaccgcag ttgcctcgct
     3841 cgcttcgggg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct
     3901 tgtctgtgag cggatgccgg gagctgacaa gcccgtcagg gcacgtcagc gggttttagc
     3961 gggtgtcggg gcgcagccct gacccagtca cgtagcgata gcggagtgta tactggctta
     4021 accatgcgcc atcagtgcgg atggtatgaa aagtgcgcca taccgggtgt gaaatgccgc
     4081 acagatgcgt aaggagaaaa tgcacgtcca gccgcttttc cgcttcctcg ctcactgact
     4141 cgctacgctc ggtcgttcga ctgcggcgag cggtatctac tcactcaaaa acgaaatgct
     4201 gttatccaca gaatcagggg ataacaccgg aaaaaacatg tgagcaaaag accaggaaca
     4261 ggaagaaggc cgcgccgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     4321 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg acttaaagat accaggcgtt
     4381 tccccccgga agctccctcg tgcgctctcc tgttccgacc ttaccgttta ccggatacct
     4441 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     4501 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     4561 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     4621 aacgccactg gcagcagcca ttggtaacta aaaagtggat ttagatacgc agaactcttg
     4681 aagttgaagc cttatagcgg ctacactgaa aggacatctt ttggtatctg tgctccactt
     4741 aagccagcta cccggttagg taatctgcca ggattagcta accttcgaaa aaccacctgc
     4801 cagggtggtt ttttcgttta caggcagcag attacgcgca gaaaaaaagg atctcaagaa
     4861 gatcctttga tcttttttct ctaaactgca atcccatcag ttcaggagag aaagatagta
     4921 taattgccct ctcttatatg gaaccaccat tcaatcacat catggggata atataagtga
     4981 aaacgttcgc attgtttatt tttacgtgta tatgtattta tagattggaa ttttaacaaa
     5041 ataaaatgga gttaatatgt tcaaatcatc agtaaagtca ataacatcga gtactatact
     5101 gaccttactt ataggatgtt caagtactga aacaaacaaa aacattgttt acaaagttgg
     5161 tggtgacagc atcattctga ataaatattg catacttaaa atgtaccctt cccaatcag
//
LOCUS       DAJTZL010000222         5208 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.223, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000222 DAJTZL010000000
VERSION     DAJTZL010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5208
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.223"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(1..255)
                     /locus_tag="OO544_003776"
                     /pseudo
     CDS             complement(1..255)
                     /locus_tag="OO544_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            319..465
                     /locus_tag="OO544_003777"
                     /pseudo
     CDS             319..465
                     /locus_tag="OO544_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(465..1622)
                     /gene="intS"
                     /locus_tag="OO544_003778"
     CDS             complement(465..1622)
                     /gene="intS"
                     /locus_tag="OO544_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HCR6769273.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISVGIVPVKAKKASSNNNSFSAIYKEWYE
                     HKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGAMERANKA
                     RRRCGEVFRYAIVTGRAKYNPAPDLADAMKGYRKKNFPFLPADQMPAFNKALATFSGS
                     IVSLIATKVLRYTALRTKELRSMLWKNVDFENRIITIDASVMKGRKIHVVPMSDQVVE
                     LLTTLSSITKPVSEFVFAGRNDKKKPICENAVLLVIKQIGYEGLESGHGFRHEFSTIM
                     NEHEWPADAIEVQLAHANGGSVRGIYNHAQYLDKRREMMQWWADWLDEKVE"
     gene            complement(1784..1858)
                     /locus_tag="OO544_003779"
     tRNA            complement(1784..1858)
                     /locus_tag="OO544_003779"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1823..1825),aa:Arg,seq:cct)
     gene            complement(1934..2866)
                     /locus_tag="OO544_003780"
     CDS             complement(1934..2866)
                     /locus_tag="OO544_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HCR6769274.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            3160..3915
                     /gene="mlaA"
                     /locus_tag="OO544_003781"
     CDS             3160..3915
                     /gene="mlaA"
                     /locus_tag="OO544_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HCR6769275.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            complement(4097..4432)
                     /locus_tag="OO544_003782"
     CDS             complement(4097..4432)
                     /locus_tag="OO544_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001315756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6769276.1"
                     /translation="MAESFATGSDHQVVNELNGERLREPNEVFKRLGRAIRYNFQVDD
                     AKFRRDNVKEIISNLFANKVDVDHPENKYKDFKDLEDNVEKRLQNRQAKYQNEINQLS
                     APGVNFDDI"
     gene            complement(4390..4782)
                     /locus_tag="OO544_003783"
     CDS             complement(4390..4782)
                     /locus_tag="OO544_003783"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6769277.1"
                     /translation="MIFSALKEIYDGDPGFIFDKISHKLRHTVTEFDESGNSEPTDLF
                     TWYGKDKKGNSLAIVIKNKNGNDYLSLGYYDQDDYHIQRGIRINGDSLTQYCSEKARN
                     ASAMLQRGLKAVKLSWQNHLQQVPIIRL"
     gene            complement(4789..5165)
                     /locus_tag="OO544_003784"
                     /pseudo
     CDS             complement(4789..5165)
                     /locus_tag="OO544_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937824.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1279 a         1201 c         1163 g         1565 t
ORIGIN      
        1 aggactgctg attgccgttg tgcagggctc cagattcaac tctctggcga actgccgtgt
       61 ttcatgcgct ctatacgctg aaccgttatc tgtcagccac tggatggact gttccggtac
      121 tttatcgcca aagcgttttt ctatcgctcc taacatgaca tcctgcactg tcgctttatc
      181 gtaacctccc gtgcttgcgg cccagtctat ggcttcacga tcgcaacagt ccagggcgaa
      241 cgtaacacgc agttttgaat cgccacgggt ttaacagaca cctcagagtc atttaagatg
      301 gcttaaagag aggtgcccat gagcggtaag cgttatcccg aagagtttaa aactgaagca
      361 gtcaaacagg ttgttgatcg cggttattct gttgccagcg ttgcaacacg tctcgatatc
      421 actacccaca gcctttatgc ctggataaag aagtacggtc cggattactc cactttttca
      481 tcaagccaat ccgcccacca ctgcatcatt tctctgcgct tatcgagata ctgagcatgg
      541 ttgtaaatac cgcgcacaga tccgccgttg gcatgtgcca gctgcacttc aatagcgtca
      601 gcaggccatt cgtgctcgtt cataatcgtg ctgaattcat gcctgaatcc gtgaccgctt
      661 tccagacctt catagccgat ttgtttgatc acaagcaata ccgcgttctc gcagattggc
      721 ttcttcttat cgttgcgccc ggcaaaaaca aactctgaga ctggtttggt gattgagctt
      781 agcgtagtga gaagttcaac cacctggtct gacatcggga ccacatgaat tttgcgtccc
      841 ttcatcacac tggcgtcgat ggtgataatc ctgttttcaa aatcgacgtt cttccatagc
      901 atggaacgaa gctcttttgt tcttagggct gtgtagcgta aaactttggt cgcaatgagc
      961 gatacgatac ttcctgaaaa tgttgccagt gctttgttga atgcaggcat ctggtctgct
     1021 ggaaggaacg ggaagttctt cttgcggtat cctttcatgg cgtctgcaag gtcaggtgcc
     1081 gggttatatt tagcccttcc ggtgacaata gcgtaacgaa aaacctcgcc gcatcttctg
     1141 cgggctttgt tggctcgctc cattgcaccg cgatcttcaa atctgcggat tacttccagc
     1201 agttgcatcg gctcaatatc ctgaatctca agaccgccga tgatgggtaa aatgtcgtca
     1261 tcaaacattt tggcaagttc agttgcatag cctactgacc agacttgctt cttgtgctcg
     1321 taccattcct tgtaaatcgc actaaatgaa ttgttgttag acgaagcctt tttcgccttt
     1381 accggaacga tgccaaccga gatgtctttc ctcgcagtcc atgccttatc ccttgcttcc
     1441 tgcaaagtca taagcggata ttttccgacg gtcaggattt tctccttacc gtcaatcttg
     1501 tagcgaagct gccatacctt tttcccggat acaggaacat aaaggtacag gccattacca
     1561 tcgagaaggc ggtatggttt ttctttcggc tttgctgctt caatctgctt aacggtgagc
     1621 atgggtaaaa atccggtggg taaaattatt ttatccactt tttacccgtc atggagtgcg
     1681 gctgtcaacg atctgacgcg aaccatgacg aaccgtgaat atacggaagg cttgatattc
     1741 aggggatttt gcggactggt acggatggga gcgaactgat aaatggtgtc ccctgcagga
     1801 atcgaacctg caattagccc ttaggagggg ctcgttatat ccatttaact aagaggacaa
     1861 tgcggcatga gtatacccgc taatggagtg cggggtaagt acgctgccgc tcgattgctt
     1921 aaaccctcgc catttatgcc gggtttttat catttttctt aatgttttcc gcacgttctg
     1981 ctttttggcg tgcttctgct ttacgcttgt tgctcatgtc gttacgaatc tgtgcatgac
     2041 tcattaacgc gaagataaag gtgccgccgc agatgttccc cgctaaagta ggtagtgcga
     2101 agggccagat gaaatcgctc cagtgcagcg taccgttaaa caccagatag aggatttcaa
     2161 cagaaccgac cacgatatgg gtggtgtcac ctagggcaat aagccaggtc atcaatataa
     2221 tcaccacaat ctttgccgca cctgctgcag gaaacatcca taccatagtg gcgatcagcc
     2281 agccggaaat gatcgcgttg gcaaacatct cgctgggggt gttcttcatc acatccatgc
     2341 cgattttgac aaatgcatcg cgagtttctt cattgaagat aggcatatat tcaaatgccc
     2401 acgcagcaat accagtcccg agaatattac ccagcagcac gactccccat aaccgcataa
     2461 gtaagccaac gttgctcatt gtcggttttt gcatgacggg tagtaccgca gtcacggtgt
     2521 tttcggtaaa taattgctgg cgggccatta tgacgataat aaaaccaaag gtataaccga
     2581 gattctccag taagaagctg cccggcacac cttccagttc gacatgaaat atcccttttg
     2641 ccagtaacga agcgcccatc gacagacccg ccgcaatggc tgaccacagt agcgccattg
     2701 cgtcgcgttc cagctctttt tcaccatcct ggcggatatg ctcatgaatt gccatcgccc
     2761 gggaggggag tcggtcttca tctatttcta tttttttgcc gcgctctttt tcttcgctct
     2821 caacttcaat ttcgtcgctg tgttgatcaa ttttgtcgtt gtccatggtc tcttctcttg
     2881 aattagcacg tatagctaaa gcgtagcggc ttttttgctc gcaactggcg ggagttactc
     2941 tgaaaatgta gaaaaggctg cgtttgcctt tttctgtttc tatagaatca agtagcctac
     3001 agggcggcga ttaccaggct atgatcaaat cagcaaatca gggcgtctgg acatcagttg
     3061 acgtgctgtt acaatcgccc acacctaaac aggcggatac ggtatcgttc cgtcatggat
     3121 ggcaaactgc ataagccata aaaaaacagg gagacattta tgaagcttcg cctgtcggcg
     3181 cttgctctgg gaactacgct tctggtgggg tgtgcgagtt ccggtacaga tcagcaaggg
     3241 cgttctgacc cgttagaagg gttcaaccgc accatgtaca acttcaactt caacgtatta
     3301 gacccgtata ttgttcgacc ggtcgctgtc gcctggcgtg attatgttcc gcaaccggcg
     3361 cgtaatggtt tgagcaactt tactggcaac cttgaagaac ctgcggtgat ggttaactac
     3421 ttcttgcagg gcgaccctta tcaggggatg gtccacttta cccgcttttt cctgaacacc
     3481 attttgggga tgggcggctt tattgatgtt gcagggatgg cgaacccgaa actgcaacgg
     3541 actgaacctc accgcttcgg tagtacgctt ggtcattatg gcgtgggtta tgggccttac
     3601 gttcagttac cgttctacgg tagcttcacg ctgcgtgatg acggtggtga tatggcggat
     3661 ggtctttacc cggttctttc ctggctgacc tggccgatgt ctgtgggtaa atggacgctt
     3721 gaagggatcg aaacccgcgc tcagctgctg gattccgatg gtctgctgcg tcagtcgtcc
     3781 gatccttata ttatggtgcg cgaagcgtac ttccagcgtc atgatttcat cgctaatggc
     3841 ggcgaactca aaccgcagga aaatccgaac gcacaagcga ttcaggatga tttaaaagat
     3901 attgattctg aataagaaac aaataaaaaa ggtgagtcgc aaaactcacc ttttttgttg
     3961 ttatccctca attaccacaa gtaaaaggag ataaaccgac aatgacccgg acgtaatgat
     4021 gtaaaaatac tgatgttcgt cactgactat ttccgatatt caggcgttga aaaatcaaaa
     4081 aactggaaaa ataattttaa atatcatcaa aattaacacc tggcgcagat agttgattaa
     4141 tttcattttg atatttcgcc tggcgatttt gaagtctttt ttcaacgtta tcttcgagat
     4201 ccttaaagtc tttgtattta ttttcaggat ggtcaacgtc aactttgtta gcgaataaat
     4261 tagaaattat ttcttttaca ttgtcgcgtc taaattttgc atcgtccact tgaaaattat
     4321 atcttattgc tcgacctaaa cgtttaaaaa cttcgttagg ttctctcagt ctttccccgt
     4381 tgagctcgtt tacaacctga tgatcggaac ctgttgcaaa tgattctgcc atgatagctt
     4441 tactgctttc aaaccacgct gaagcattgc tgaagcattc ctggcttttt cactacaata
     4501 ttgggtgaga ctatcaccat taatacgaat tcctctttga atgtggtagt cgtcctgatc
     4561 gtagtaaccg agagataagt aatcatttcc gtttttattt ttaattacaa tagcgagaga
     4621 gttgcctttt ttatctttac cgtaccaggt aaataagtcc gttggttcgc tattcccgct
     4681 ctcatcaaat tctgttaccg tatgtctgag tttgtgtgat atcttatcga agataaaacc
     4741 aggatctcca tcatatattt cttttaatgc gctgaagatc atttccttgt tattgatatc
     4801 atgagtcttc tcaactatgt tgtcattaat gatagtacgg acgagttctt tgtctttata
     4861 gttcgcaggt aattctattg gttcttggtt gtcgatacca atagtaatat ggtctgtatc
     4921 attggagcca atatcaatat aagtaactga ggaattatta atattaaatc ttaaggatcg
     4981 tcgtttactc gtatggcgtt ttttataagc tcaagtagat gtttaataca ggtgcttatt
     5041 ccttccttac cattaaaaag gtttttgagc tcttgtacta atccattaac ttcatcattg
     5101 ttattttcat taatggattc tgggtaagtt gcgctggtat tgttgattga aatagatggt
     5161 agcatgattt tattccattt tgattgattt gttacaaata tatattac
//
LOCUS       DAJTZL010000223         5205 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.224, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000223 DAJTZL010000000
VERSION     DAJTZL010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5205
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.224"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            76..417
                     /locus_tag="OO544_003785"
     CDS             76..417
                     /locus_tag="OO544_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HCR6769278.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASKIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQRQQAEKAQEKEQRLSLNVSLLNP
                     VENTTHIDIYI"
     gene            complement(408..1334)
                     /locus_tag="OO544_003786"
     CDS             complement(408..1334)
                     /locus_tag="OO544_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6769279.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFCIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGAPLYQI"
     gene            1424..2422
                     /locus_tag="OO544_003787"
     CDS             1424..2422
                     /locus_tag="OO544_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HCR6769280.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(2419..2637)
                     /locus_tag="OO544_003788"
     CDS             complement(2419..2637)
                     /locus_tag="OO544_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HCR6769281.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(2639..4654)
                     /gene="ligA"
                     /locus_tag="OO544_003789"
     CDS             complement(2639..4654)
                     /gene="ligA"
                     /locus_tag="OO544_003789"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HCR6769282.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEVLEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(4725..>5205)
                     /gene="zipA"
                     /locus_tag="OO544_003790"
     CDS             complement(4725..>5205)
                     /gene="zipA"
                     /locus_tag="OO544_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HCR6769283.1"
                     /translation="VAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLL
                     NSIQQAGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQ
                     VPSYGDELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
BASE COUNT         1206 a         1450 c         1264 g         1285 t
ORIGIN      
        1 tgtaagattg atttttcttc tcaagttcct tgttcttctg ccgatattac ctcgtacagt
       61 gacgaggact gaacaatggc cactatctct tcaacttcaa ttccttccat acaaacacag
      121 agtagtaaca gggcatcaca aggtagcgat gttgcttcaa aaatcgcgcg gataagtcag
      181 caaatcatca aactcactca acaaattaaa gagattgtag acactagcgg cagtgctgaa
      241 gacaaacaaa aacaggccga attgattcaa cagcaaatca ccctactgga aacacaactg
      301 gcgcagttgc agagacagca ggcagaaaag gcgcaggaga aagagcagcg tttgtctctg
      361 aatgtatccc ttctaaatcc tgttgaaaat acgactcata tcgatatcta tatctgatac
      421 aacggagccc cttcccgacc agccattagc gcattgccct ggtctcgtac cacgtcccat
      481 aacgcttcag cggcggtgga aagtgaacgg ttcttcctgc gcaccagcat caactggcgt
      541 tcaacaaccg gcgtaatgcg ctttaccacc agtgggctac cttcaggcaa tggtagcgcc
      601 agtgcgggaa gaatactgat gccgatgcct gcggctacca tcgggaacag cgtcgccgga
      661 tgtccaatct cctgcacaat attcgcctga ataccattgc gcgccagcgc tgcgtcaatc
      721 agcggtcggc tgcctgacgc gtaatcctgc aacaccaatt tcgctccctg taatgcctgc
      781 cagggtacat aatcttccac ggccaaagcg ctatcgcgat ggcaaagcag aaagaaaggc
      841 tcggaaagaa tcgcttcaca ttgcagatcg cccactgggc ctggatcgat gacgatgcaa
      901 aaatcgacat ctccctggcg aatactttcc atcacccact gctgcggacg atcgtgcagg
      961 acaaactgaa tatctggata gcggcgatgg ctttcagcaa tgcattgcgg aataagatgc
     1021 gcggaaatgg tctggctggc agcgacccga acttttccgc tcagttgttg ccccatacgc
     1081 ccggtatcgc gcaacgtgct gttaagttca tccagcagtc gctcaagacg caaagccaac
     1141 tgctgccctg catctgtaag caccacttca cgcgtggttc tgtccagcag gcgaacacca
     1201 gtatgatttt ccagctcctt cacactgtga cttactgccg actggctcaa gccgatacgc
     1261 tctcctgcac gactaaaact tttctcctgc gctactgtga cgaaaacctt taattgtttt
     1321 aaagaataat tcatctgttt tcttcatgaa tggatgcaat aaatcaattt tatttctcaa
     1381 actggaagaa gcacaataga accatcgatc atctggagtc tttatgaaac tttttcgtat
     1441 cctcgatcct ttcaccttaa ccctgatcac ggtggtgttg ctggcctctt tctttccggc
     1501 cagaggcgat ttcgtcccct tctttgaaaa tctgaccacc gcagctattg ccctgctgtt
     1561 ctttatgcac ggcgcgaagt tgtcgcgtga ggcgattatt gctggcggtg gtcactggcg
     1621 actgcatttg tgggtaatgt gcagcacctt cgtgctgttt ccgattctgg gtgtactgtt
     1681 tgcctggtgg aaaccggtaa atgtcgaccc gatgctctac tccggttttc tctacttgtg
     1741 cattctcccg gctaccgtgc agtctgcaat cgccttcacg tcaatggcgg gcggtaacgt
     1801 cgcggcggcg gtttgttctg cgtcagcatc cagcctgctg gggattttcc tttcaccatt
     1861 gctagttggt ctggtgatga atgttcacgg tgcagggggc agccttgagc aggtcggtaa
     1921 aattatgctg caactgctgc tgccgtttgt gttggggcat ctttcccggc cgtggattgg
     1981 tgactgggtg tcgcgcaata aaaaatggat tgcgaaaact gaccagacgt ccattctgtt
     2041 ggtggtttat acagcgttca gcgaagccgt cgttaatggt atctggcata aagttggctg
     2101 gggatcattg ctgtttatcg tggtggtcag ctgcgttctt ctggctatcg tgattgtagt
     2161 taacgtcttt atggcacgcc gactgggctt caataaggca gatgaaatta ctatcgtctt
     2221 ttgtggttcg aaaaagagtc tggcaaatgg catcccgatg gcaaacattc tgttccccac
     2281 atcggtgatc ggtatgatgg tgctgcccct gatgattttc catcagatcc aattgatggt
     2341 ctgtgcggtg ctggcgcgtc gatacaaacg ccagaccgaa cagttacagg cgcagcagga
     2401 aagcagcgcc gataaagctt aaagcggacg cttcaggggc tggaccagtt gcgtcagccc
     2461 ctcggtttta atcagcagcg tgatttgcat tagctcaccg agcttccctg ccgggaactc
     2521 atctttgcgg gcaaaccaca gcagatactc ctccggcagg tcgattaagc gacgcccttt
     2581 gtatttgcca aacggcatta tcgtattggc tatttcaatc agctgctctt tttccatctc
     2641 agctacccag caaacgcagc atttccgctt cgtcgatgac ttcaatgccc agttcctgcg
     2701 ccttcgccag tttagatcct gcagcttcac ccgctatcac cagatcggtt ttcttcgaca
     2761 cgctgcccgc gactttcgcg cccagttcga ccagtcgagc tttagcgtca tcacgcgaca
     2821 tctggcttaa gctgcccgta agcaccacgg ttttaccagc aaacgggctg tcaatctctt
     2881 ccgcgttgat aacgatcggc gcaggccagt gaacaccttc cgccaacagc tcgctgatga
     2941 cattgcggtt gctttcttcg gcaaagaagt tgtgaacgtg ggatgcaacg acaatgccaa
     3001 catcaggcac cttttgcagc tcttcaatcg aagcggcttc cagcacttcc agcgtgccga
     3061 aatatgccgc cagacctgct gcggtggcct cgccgacttc acggatgcca agtgcataga
     3121 ggaagcgagc aaaggtggtt tctttcgctt tttccagcgc gttaaccacg ttttgtgccg
     3181 attttggccc catacgctcc agtccggtca gtttgcctgc ggtgagtttg aacagatctg
     3241 ccggagtgtg gacatattct ttttcaacca gctgatcgat gattttgtcg cccattccgt
     3301 caacatccat cgcacggcgg gaaacaaagt gtttcagcga ctctttacgc tgcgcaccgc
     3361 aaatcaggcc accggtacag cgggcaaccg cttcaccttc cacacgctca acgtcagaac
     3421 cacataccgg acaatgcgtc gggaatacaa cctcacgggt atcttccggg cgttcagaaa
     3481 gcacgacgtt aaccacctgc gggatcacgt cgccagcgcg gcgaatcacc actttatcgc
     3541 caatgcgtaa accaagacgt tcgatttcat ccgcattgtg taaggttgcg ttactcacca
     3601 gcacgccagc aacatggaca ggttccagac gcgcaacagg cgtaatcgcg ccagtacgcc
     3661 caacctgaaa ctcgacgtca cgcacaaagg tcatctgctc ctgcgccggg aatttaaacg
     3721 ctaccgccca gcgcggggca cgcgcgacaa agccaagctg ctcctgctgt tccagtgagt
     3781 tgaccttaat caccacgccg tcgatatcaa agcccagcgt cgggcggtct tcttccactt
     3841 tgtgatagaa cgccagcact tcttccgccg attcacaaag cgttacccga tcgctgaccg
     3901 gcaaccccca ctttttaaat tgcagtaaac ggccaagatg agtatccggc agctcgccac
     3961 cttccagaac accaacgcca tagcagaaaa aagtgagcgg tcgcttcgct gtaatacgcg
     4021 gatcaagctg acgcagtgaa ccagctgccg cattacgtgg gttagcaaaa actttcccgc
     4081 ccgtgcgtcg cgcatcttcg ttaatctttt cgaaccccgc ctgcggcagg aacacttcac
     4141 cacgcacttc cagacgcgcc gggatattct ctccgtgcag cttcagcgga atggcgcgaa
     4201 tagtacgcac attagacgtg atatcttccc cggtggtgcc atcgccacgg gtcgcggcac
     4261 tgactaaaac gccattttca tacagaatac tgacggcaag gccatccagc ttcagctcac
     4321 agcaccaggt gactttctcg ttgtttttca gacggtcctg cacacgtttg ttgaaagcaa
     4381 gaaagctttc ttcatcaaaa acgttatcca gtgacagcat tggtacttca tggcgtatct
     4441 ggctgaaagc cgccagcggc gcagcaccta cacgttgggt aggcgaatca ggcgtaatca
     4501 gttctggatg tttggtttcc agctcgcgca gttcgcgcat cagcctgtcg tattcagcgt
     4561 cgggaatttc cggcgcatcc atcacatgat aaagatattc atgatggcga agcgtcgttc
     4621 gcagttctgt cagttgttgt tcgattgatt ccatatcgca ccatcaatgc taaaaacccc
     4681 cgacaagcgg gggttcgaag aggagttaat ttgccttaag tgtatcaggc gttggcgtct
     4741 ttgacttcgc ggatgatgtc ctggtactcg cgcaatttct gcggagtcat catacggcgc
     4801 tgatcgtcaa gcacgacacc gcccacttca tcggcaatat gctgcgcaga ttgcagcatc
     4861 agcttgaagt tctgcagctc gtcaccgtaa gacggtacct gcataaagat agtgacaccc
     4921 ggagtagtga aatccttcat ttcaggatca aaggttcccg gtttcaccat attcgccagg
     4981 ctgaataacg ccgggccgct gccatccggg ctaagatgac gatggtaaat attcatatcg
     5041 ccaaaaatga agcccgcttg ttgaatgctg ttaagaagca gttcaccgtt tagctcgcta
     5101 ccgtgatgcg ccgcgacgtt catgataatc accgcttctt tgcgcttcgg tttatccata
     5161 actggagccg gttccgctac aggctcaggc tgtggtgccg ctacg
//
LOCUS       DAJTZL010000224         5114 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.225, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000224 DAJTZL010000000
VERSION     DAJTZL010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5114
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.225"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..379
                     /locus_tag="OO544_003791"
     CDS             <1..379
                     /locus_tag="OO544_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001340328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6769284.1"
                     /translation="KKTNIYFDDNFLNIYKRSQDRAPQIISDLKKHNIEIGEKLNDCR
                     KKLNISDVKLTYYWFVTDTGGVDYLDGVLTYNFQGKNRAENFHKQLLQYAYINYSKNQ
                     IPRDFVTSIKNSIYYKIEDCTKF"
     gene            complement(575..2125)
                     /locus_tag="OO544_003792"
     CDS             complement(575..2125)
                     /locus_tag="OO544_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001624483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HCR6769285.1"
                     /translation="MASYHCTVKAGAKGSALKHADYISRSGEYKSYKSREDLEFSSSG
                     NMPSWAKKNPAELWKAADEFERKNGTAYREIEIALPRELTREQRIELVEDFVQKELGD
                     RHAYQYAIHNPPGAIDGKEQPHAHIMFCERINDGIERDPQQFFKRANSKSPERGGAKK
                     ASIPQTAGERKAALVALRSRWADVQNEHLARHGHESRVDHRSLKEQGINRTPEVHLGP
                     VQAASLNGEQIVAIQERRNAERELKTARDAANAIQQEQEQKQKIKAVEPVRSARSPEL
                     LLQYRKVMKTVIQGEARLARLGDANPNALKEHKLLQNAKAKKDSLSEWSRRIYEGARY
                     LDKLGRNVVSAQRELRELQEQRNALNGIRGLFRGADKREIDARILEQKSVLETAEHER
                     NEFRNKLQQAESEWDKENAAFKRTEGYKYVGELDRYREREILAAASLENTRQKVAEEV
                     SVARSQMLSLEPELSISGDEKAQMRHELLAEMAQERQQQEEKALCIQRSWARGVSRSN
                     ERDQDMER"
     gene            2297..2743
                     /locus_tag="OO544_003793"
     CDS             2297..2743
                     /locus_tag="OO544_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="HCR6769286.1"
                     /translation="MTNREEWLSAKIAYINGLKSPSEQQRLLVLLAEKKNRTTTDEKT
                     LSALIRAEKTAEKAAAAKARVTAIIAAERKAAARAERKARDHELYKAAGLMIVAGLVD
                     SKTGKPKFSAAELVGALAGIAELPHNHPKWQEWEKRGKELLTKDSA"
     gene            complement(2937..3872)
                     /locus_tag="OO544_003794"
     CDS             complement(2937..3872)
                     /locus_tag="OO544_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009440807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HCR6769287.1"
                     /translation="MSAALQYFEENLPHRPYHTDELAFGLRISGKGRALLARYIQQNQ
                     PHAQFWLVFDVDRVGAAIDWSDRNAPAPNITVKNPENGHAHLLYALNLAVRTAPDASV
                     KALKYAAAIERALCEKLDADVNYSGLICKNPFHLEWQVMEWREEAYALDELADYLDLR
                     ASERRSVDKHYGLGRNCHLFEMTRKWAYRAIRQGWPAFSQWLDAVIQRVEMYNASLPV
                     PLSPPECRAIGKSIAKYTHRNFTPESFAQYVADTHTPEIQAKRGRKGGIAKGEAYDDK
                     RFMALCMLENGYSQKAIAAMLNVHRNTIRNWAMHK"
     gene            4384..>5114
                     /locus_tag="OO544_003795"
     CDS             4384..>5114
                     /locus_tag="OO544_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001360357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6769288.1"
                     /translation="MGKMNINFKHILPLLSSFFISGCDSDGISKVKSFVYYDIDDSMT
                     VGKAFDTRSDCINGTWSEKEDDRGRTIVTYTCDISESGLKIINSAIMQEPEDKAEYSI
                     AQNNNSIKSTNESLEEYKQKTPLVEKTKEVIIDHIRKLNSAFNEDPMIYSKLTISPYL
                     DRMLYLKDHNDNALNEICGGYEYIQSADSYSWRDVSEEYAKESCEKHIYKVYQSFKKN
                     ASPLITKNFPGFYERVPPCENADEC"
BASE COUNT         1418 a         1244 c         1126 g         1326 t
ORIGIN      
        1 aaaaaaaaca aatatatact ttgatgataa ctttctaaat atctacaaaa gaagccaaga
       61 tagagcacct caaataattt ctgatttaaa aaaacacaat atagaaatag gtgaaaagtt
      121 aaatgattgt aggaaaaaac taaatatctc agatgtcaaa ttaacctatt actggtttgt
      181 aacagatacc ggaggagttg attatttaga tggggtgttg acatataatt ttcagggaaa
      241 gaatagagct gaaaactttc ataaacaact attacaatat gcatacatta actacagcaa
      301 aaatcaaata cctcgggact ttgtaacatc aataaaaaat agtatttatt ataaaataga
      361 agattgcact aagttttagt ccttccctta caggtggtta cacatataac cacctgcaaa
      421 tatttaacac ctgttaactt cattaatctg aactgtgagt aaatcaaaac gacaattttt
      481 ctcattcaga aaacgtttca cgggacgcgg aattttttcg cgttccgtga aacgttggcg
      541 gggttcgggg cgcagtcccg atattgctac attttcaacg ctccatatct tgatcacgtt
      601 cgttactacg tgataccccc ctggcccagg atcgctgtat acacagtgct ttttcctcct
      661 gttgctgccg ttcctgcgcc atttctgcca gtaactcatg tcgcatttga gctttttcat
      721 ctccagatat actgagttca ggctccagag ataacatctg gctacgggca acggatacct
      781 cttcagcaac cttttgcctg gtgttctcta aagatgccgc cgccagaatc tcgcgttctc
      841 tgtacctgtc cagctcccca acgtacttat atccctcggt acgcttaaaa gcagcgtttt
      901 ctttgtccca ctcggattcg gcttgctgta gcttgttcct gaattcattc ctttcatgtt
      961 cagcggtttc cagcacactt ttttgctcca gaatacgcgc atcaatctcc cttttgtcag
     1021 ccccccgaaa caggccacga atcccattca aagcattgcg ttgttcctgt agctcccgca
     1081 gctccctttg tgcggaaaca acattacgcc ccagtttgtc taaataccgt gccccttcat
     1141 atattcgtct actccattcg gaaagagagt cttttttagc tttcgcattc tgaagcagct
     1201 tatgttcttt gagcgcattg ggattggcat cccctaaacg agcaagcctt gcctctccct
     1261 gaatgaccgt tttcataacc ttgcggtact gaagcaataa ctccggtgaa cgggctgaac
     1321 gaacaggctc aactgccttg attttttgct tttgctcctg ttcctgctga atggcatttg
     1381 cggcatctct ggcggtcttg agttcccgct cggcattcct ccgttcctgg attgcaacaa
     1441 tctgctcccc attcagactg gcggcctgaa cggggccaag atgaacttcc ggcgtgcgat
     1501 tgataccctg ttctttcaga gaacgatgat ctacgcgcga ctcgtgaccg tgtcgcgcaa
     1561 ggtgctcatt ctgcacatct gcccagcggg aacgcagcgc aacaagggcg gcttttcgct
     1621 ctccggctgt ctggggaata ctcgcttttt ttgcgcctcc ccgctctggt gatttgctgt
     1681 tggcacgttt aaaaaattgc tgtggatcgc gttcaatacc atcattaatc cgctcgcaaa
     1741 acatgatatg cgcgtgtggc tgttcctttc cgtcgattgc acctggcgga ttgtggatcg
     1801 cgtactggta ggcatggcga tcgccaagtt ccttttgcac gaaatcttca acaagttcta
     1861 tccgttgttc gcgtgtcagt tcacgcggta acgcgatctc aatttcacgg tatgcagtcc
     1921 cgttttttcg ttcaaattca tctgcggctt tccatagctc tgcgggattt ttcttcgccc
     1981 atgatggcat gttaccggat gacgaaaatt ccagatcctc ccgcgatttg taactcttat
     2041 attcgccgga tctggaaatg taatcagcat gttttaaggc actgcctttt gctccagctt
     2101 tgacagtgca atggtagctt gccatctgcc ttttagccct ccgcaggagc gcacagtttc
     2161 tcgacgaagg agagaaacta ctaagtgcgc atccctgaat taatcatgga tgattttacg
     2221 gtctcacacg caggcaaaca aggtgatatt atgcataata acaactcaca ttaacggcaa
     2281 aagtgcagag gtaagaatga caaatcgtga agagtggtta agtgcaaaaa ttgcctacat
     2341 caacggactg aaatcaccat cagagcagca gagattactt gtgttactgg cagagaaaaa
     2401 aaaccgaaca acgacggatg aaaaaacgtt gtccgcactg atacgggcag aaaaaaccgc
     2461 agagaaagca gccgcagcaa aagccagagt aacggcaatc atagcggcag aacggaaggc
     2521 agccgcaagg gcggaacgaa aggcgcggga tcatgaactc tacaaggccg caggcctcat
     2581 gattgtggca ggactggtgg acagcaaaac cggaaagcca aagtttagtg ctgccgaact
     2641 ggtaggcgca ctcgcaggaa tcgcagagct accgcacaac catccaaaat ggcaggaatg
     2701 ggaaaaacgc gggaaagagc tactcaccaa agacagtgcg taagagcctc cgttcacgtt
     2761 ccagttccct ggtgcttcct cgctcattcg ctcgctacgc tcggtcatga gactgcggcg
     2821 agcacgacca cctcaatcat acggtaagcc gcaccattgc tgggctgaaa ggagaaaacc
     2881 gccggaaagc ccgctgctca aaaagacgcc gaaaaggcgc tgttatctga tataggctat
     2941 ttgtgcattg cccaattacg gatcgtatta cgatgtacat tcagcatcgc cgcaatagct
     3001 ttctgagaat atccattctc cagcatacat agcgccatga aacgcttgtc atcgtaggct
     3061 tcgcctttag cgatgccacc tttccggcct ctcttggcct gtatttctgg tgtgtgcgta
     3121 tcagccacat actgtgcgaa actttccggc gtgaagttcc tgtgcgtgta tttcgcaatg
     3181 ctcttgccaa tagcccgaca ttcaggcgga gaaagcggaa cgggaagcga tgcgttgtac
     3241 atttcgacac gctgaatcac agcatcaagc cactgtgaga atgctggcca gccctgccga
     3301 atcgccctgt aagcccattt gcgcgtcatt tcgaacagat ggcagtttcg ccccagcccg
     3361 taatgtttat cgacgctacg acgctctgag gctctcaaat cgagataatc agccagttca
     3421 tcgagggcgt aggcttcctc gcgccactcc atcacctgcc attccaggtg gaacggattt
     3481 ttgcaaatca gtccgctgta gttcacatct gcgtcaagtt tctcacacag cgcacgttca
     3541 attgcagcgg cgtatttcag cgccttaacc gacgcatcag gcgcggttct cacggcaaga
     3601 ttgagcgcgt agagcaggtg agcgtgcccg ttctcgggat tttttacggt gatgttcggc
     3661 gcgggggcgt tccggtcgct ccagtcaatc gcagctccta cgcggtcaac gtcaaaaacc
     3721 agccagaact gcgcatgagg ctgattttgc tggatgtagc gagcaagcag cgcacgccct
     3781 ttgccggaga tacgaagacc gaaagcgagc tcatccgtgt gatagggacg gtgaggtaaa
     3841 ttttcttcga agtattgaag cgcggcgctc atgtgttacg attccttcca ggttatcgac
     3901 cgacatcgcc cctgtatctt cgtgaaagtc ttgccggata gacacgaatt tcaggggcgt
     3961 ttgtttttca gtcgcttaca ctacccccaa cgacaaaacc tgtcgagtct tcccgcaaac
     4021 ccgatccatt taccataact cccgttgtgc gcccccctta aaacgccctc agtcgctttc
     4081 tgtcgcaaaa aacgcaaaaa gacagggaaa caacaggcca gaccaaaaaa acgcgccaga
     4141 attgttttca gggggctgca taactatgca cgaaagcgat tcatgtccgg tttcactcgt
     4201 tttgaacagc gaacaggtca aaacagcaaa ctttctcatc cagaaaaagg tgaactggtg
     4261 cgaaatcttt tcgaactgcg taagacgttg agcacaaaca tagtaagtcg gtttatcggt
     4321 atgtatggat tccattaaca tatcgcctgt attaatattt cctcgttatt gttagaaata
     4381 aaaatgggaa aaatgaatat taactttaaa catatccttc ctttattgtc gtcatttttt
     4441 atttcaggat gcgactctga tggaataagc aaggtaaagt ctttcgtata ttacgacatt
     4501 gacgactcaa tgactgttgg taaagccttc gatacaagga gtgattgcat caatggcaca
     4561 tggagtgaaa aagaggatga tcgaggaaga accatcgtca catatacctg tgatattagt
     4621 gaaagtggcc taaaaataat aaattcagcc attatgcaag aacctgaaga taaggctgaa
     4681 tattctatag cacaaaataa caacagcata aaatcaacca atgaatcact agaggaatac
     4741 aaacagaaaa cgcctctggt ggaaaaaaca aaagaggtca taatagatca tataagaaag
     4801 ctaaacagtg cttttaatga agatccaatg atatatagca aactcacaat tagcccttat
     4861 ttagacagaa tgctatatct aaaggaccat aatgacaatg cgctaaatga aatttgtggt
     4921 gggtacgaat acattcaatc tgccgatagt tacagttggc gtgatgtaag tgaagagtat
     4981 gcaaaagaat catgcgaaaa gcatatatat aaagtatatc aatcgtttaa gaaaaatgca
     5041 tcaccattaa taactaaaaa cttccccggt ttctacgaac gtgtcccccc ctgtgagaac
     5101 gcagatgaat gcat
//
LOCUS       DAJTZL010000225         5068 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.226, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000225 DAJTZL010000000
VERSION     DAJTZL010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5068
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.226"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..348)
                     /gene="mhpC"
                     /locus_tag="OO544_003796"
     CDS             complement(<1..348)
                     /gene="mhpC"
                     /locus_tag="OO544_003796"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308431.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HCR6769289.1"
                     /translation="MSYQPQTEAATSRFLNVEEAGKTLRIHFNDCGQGDETVVLLHGS
                     GPGATGWANFSRNIDPLVEAGYRVILLDCPGWGKSDSIVNSGSRSDLNARILKSVVDQ
                     LDIAKIHLLGNSMG"
     gene            complement(366..1310)
                     /gene="mhpB"
                     /locus_tag="OO544_003797"
     CDS             complement(366..1310)
                     /gene="mhpB"
                     /locus_tag="OO544_003797"
                     /EC_number="1.13.11.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-carboxyethylcatechol 2,3-dioxygenase"
                     /protein_id="HCR6769290.1"
                     /translation="MHAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPE
                     LVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSAAGELPVPVELAEACAHAVMKSG
                     IDLAVSYCMQVDHGFAQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGR
                     FTSTLNKRVLFLGSGGLSHQPPVPELAKADAHMRDRLLGSGKDLPASERELRQQRVIS
                     AAEKFVEDQRTLHPLNPIWDNQFMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWV
                     AAFAAISAFGNWRSEGRYYRPIPEWIAGFGSLSARTEN"
     gene            complement(1312..2976)
                     /locus_tag="OO544_003798"
     CDS             complement(1312..2976)
                     /locus_tag="OO544_003798"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HCR6769291.1"
                     /translation="MAIQHPDIQPAVNHSVQVAIAGAGPVGLMMANYLGQMGIDVLVV
                     EKLDKLIDYPRAIGIDDEALRTMQSVGLVENVLPHTTPWHAMRFLTPKGRCFADIQPM
                     TDEFGWPRRNAFIQPQVDAVMLEGLSRFPNVRCLFSRELEAFSQQDDEVTLHLKTAEG
                     QRETIKAQWLVACDGGASFVRRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDP
                     VRPYVSAALPHAVRRFEFMVMPGETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYT
                     HNARLAQRFRIDRVLLAGDAAHIMPVWQGQGYNSGMRDAFNLAWKLALVIQGKARDAL
                     LDTYQQERRDHAKAMIDLSVTAGNVLAPPKRWQGTLRDGVSWLLNYLPPVKRYFLEMR
                     FKPMPQYYGGALVREGEAKHSPVGKMFIQPKVTLENGDVTLLDNAIGANFAVIGWGCN
                     PLWGMSDEQIQQWRALGTRFIQVVPEVQIHTAQDNHDGVLRVGDTQGRLRSWFAQHNA
                     SLVVMRPDRFVAATAIPQTLGNTLNKLASVMTLTRPDADVSVEKVA"
     gene            3018..3851
                     /gene="mhpR"
                     /locus_tag="OO544_003799"
                     /pseudo
     CDS             3018..3851
                     /gene="mhpR"
                     /locus_tag="OO544_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414880.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator MhpR"
     gene            3928..5010
                     /gene="lacI"
                     /locus_tag="OO544_003800"
     CDS             3928..5010
                     /gene="lacI"
                     /locus_tag="OO544_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor LacI"
                     /protein_id="HCR6769292.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNT
                     QTASPRALADSLMQLARQVSRLESGQ"
BASE COUNT         1165 a         1459 c         1377 g         1067 t
ORIGIN      
        1 gcccatcgag ttgcccagca ggtggatttt ggcgatatcc agttgatcca ccacgctttt
       61 caggattcgt gcattaagat ccgatcgtga accactatta acgatcgaat cgcttttgcc
      121 ccaacccgga caatccagca ggatcacccg atagcccgcc tctaccagcg gatcgatatt
      181 gcggctgaag ttcgcccagc cagtagcacc cgggccggaa ccatgcagca ggacaacggt
      241 ttcgtcgcct tgtccgcagt cattaaaatg gatgcgcagc gttttacccg cttcttctac
      301 attcagaaaa cggctggtgg cggcttcggt ttgtggctga taactcatca tcttctcctg
      361 catattcagt tctctgttct ggcgcttaac gagccaaatc cggcaatcca ctccgggatt
      421 gggcggtaat aacgcccttc gctacgccag ttgccaaatg cagaaatagc ggcaaaagcg
      481 gcgacccagg ttttgatttc atgtgtcgac tttccggcaa tggcggaaag ctcttcgtta
      541 ctgacggcat ccagttcctg tatgcgtccc tgctccagca aagtcatgaa ctggttatcc
      601 caaatcgggt tgagcggatg cagagttctc tgatcctcaa caaacttctc agcggcgcta
      661 atcacccttt gctgacgcaa ttcgcgctca ctggcgggta aatctttccc gctccccaac
      721 agacggtcgc gcatatgggc atcggctttc gccagttcgg gcaccggcgg ctgatgggaa
      781 agcccaccgg aacccaggaa cagcacgcgt ttattgagag tgctggtgaa acgtccaatg
      841 gcttcaccca acatgcgggt acgctggaaa ccgggcagcg gcgtggcgac accgttgatg
      901 aacacaggca gaactggcac cttatccagc ccaccgagca ggaactccag cggctgggcg
      961 aacccgtggt ccacctgcat acagtaagaa acggcaagat cgatcccgct cttcatgaca
     1021 gcatgcgcac aggcctccgc cagctccaca ggcacgggca gctctcctgc cgcactgccg
     1081 aaatcaccaa ttgccgtcgc tccaacgcct aaacagaacg gtggcatcac gtcatagaaa
     1141 aagccgttgt agtgatctgg cgcaaacagc accaccagtt caggggagaa agccgcaata
     1201 cgctcgcggg cgctggcaat cacgccattg acctcatcga gtacctcttg cgccgggtcg
     1261 acatacccca ccaacggcga gtgggaaaga cagtgaagat aagcgtgcat atcaggctac
     1321 cttttcgaca gaaacgtcgg catcagggcg ggtcagcgtc atcaccgacg ccagtttatt
     1381 cagcgtattg cccagggttt gcggaatggc ggtggcggca acaaagcgat ccgggcgcat
     1441 caccaccagc gaagcattgt gttgcgcgaa ccagctacgc agacgacctt gcgtatcgcc
     1501 cacgcgtagc acgccgtcgt ggttatcctg tgcggtatga atttgcactt ccggcaccac
     1561 ctgaatgaag cgtgtgccca acgcgcgcca ctgctggatt tgctcgtcgc tcatccccca
     1621 cagtggattg catccccagc caattaccgc gaagttcgcg ccgatcgcgt tatcgagcag
     1681 cgtcacgtcg ccgttttcca gcgtgacttt cggctgaata aacatcttgc cgaccggaga
     1741 gtgcttcgct tcgccctcac gcaccagcgc accgccgtaa tattgcggca tcggcttgaa
     1801 gcgcatttcg aggaagtagc gttttactgg cggcagataa ttcaacagcc aggaaacgcc
     1861 gtcacgtaac gtaccctgcc agcgtttcgg cggagccagc acgttgcccg ccgtcacgga
     1921 caggtcaatc atcgctttgg cgtgatcgcg acgttcttgt tgataggtat cgagcagcgc
     1981 atcgcgggct ttcccctgga taaccaacgc cagtttccag gcgaggttaa aggcgtcgcg
     2041 cataccactg ttatagccct gcccctgcca caccggcatg atgtgcgcgg catcgcccgc
     2101 cagcagtacg cgatcaatac ggaaacgttg cgccagtcgc gcgttatggg tgtagacacg
     2161 ctggcgaatc aattcaacat tgtccggatt aggcagcact ttgcttaaca gcttgcgcat
     2221 attttgcggc tcacgcagct gctcttcggt ttctcccggc atcaccataa attcaaagcg
     2281 acgtaccgca tgaggcagcg cggcagaaac atacgggcgc accggatcgc aacacaaata
     2341 gatatgcggc gtacttaacg gatcgttggc gatatctacc acaatccact gatttggcgc
     2401 agttttacct tcaaacggca cattcagggt gcgacgaaca aaacttgccc caccatcaca
     2461 ggccaccagc cactgggctt tgatcgtttc ccgctgccct tctgccgttt tcaggtgcaa
     2521 ggtcacttcg tcatcttgct ggctgaaggc ctccagctcg cgggaaaaca agcagcgcac
     2581 attcggaaaa cgcgacaacc cttccagcat caccgcatcg acctgtggct gaataaaggc
     2641 gttacggcgc ggccagccaa attcatcggt cattggctga atatcagcaa aacagcggcc
     2701 ttttggggtg agaaaacgca tcgcgtgcca cggtgtagtg tgcggcagaa cattctcgac
     2761 caggccgacc gactgcatgg tgcgcagcgc ctcgtcatca ataccaatcg cacgcgggta
     2821 gtcgatcaac ttatcgagtt tctccaccac cagcacgtca atgcccatct gaccgagata
     2881 gttcgccatc atcagcccaa ccggaccggc accagcgatc gccacctgaa cgctatggtt
     2941 aacagcaggc tggatgtcag ggtgttgtat tgccatttct gcacaacgtt gttttaacta
     3001 tagaaatgtc aattaatatg cagaacaatg agcagacgga atacaaaacc gtgcgcggct
     3061 taacccgcgg tctaatgtta ttaaatatgt taaataaact tgatggcggt gccagcgtcg
     3121 ggctgctggc ggaactcagc ggcctgcatc gcaccactgt gcggcgactg ctggagacgc
     3181 tgcaggaaga gggatatgtc cgccgtagcc cctccgatga cagttttcgt ctgaccatca
     3241 aagtgcggca attaagcgaa ggatttcgtg acgaacagtg gatttctgca ctggcggccc
     3301 cgctgctggg cgatctgttg cgcgaagtgg tatgaccaac agatgtgtcc accctggatg
     3361 ttgatgcaat ggtggtacgc gaaaccaccc accgtttcag ccgcttatcc tttcaccggg
     3421 caatggtcgg gcgacgttta ccgctcctga aaaccgcctc gggcctgacc tggctggcct
     3481 tttgcccgga acaagaacgc aaggaattaa tcgaaatgtt agccgcccgc cccggtgatg
     3541 actatcaact ggcacgagaa ccgttaaagc tgcaagccat tctggcgcgc gcgcgcaaag
     3601 agggttacgg acagaactac cgcggctggg atcaggagga gaagatcgcc tctatcgccg
     3661 taccgctgcg cagtgaacaa cgggtgattg gctgtctgaa tctggtgtat atggcgagcg
     3721 caatgaccat tgaacaggca gcggaaaagc atcttccggc gctacaacgg gtagcaaaac
     3781 agatcgaaga aggggttgaa tcgcaggcta ttctggtggc cggacggcga agcggcgtgc
     3841 atttacgttg acaccatcga atggcgcaaa acctttcgtg gtatggcatg atagcgcccg
     3901 gaagagagtc aattcagggt ggtgaatgtg aaaccagtaa cgctatacga tgtcgcagag
     3961 tatgccggtg tctcttatca gaccgtttct cgcgtggtga accaggccag ccacgtttct
     4021 gcgaaaacgc gggaaaaagt ggaagcggcg atggcggagc tgaattacat tcccaaccgc
     4081 gtggcacaac aactggcggg caaacagtcg ttgctgattg gcgttgccac ctccagtctg
     4141 gccctgcacg cgccgtcgca aattgtcgcg gcgattaaat ctcgcgccga tcaactgggt
     4201 gccagcgtgg tggtgtcgat ggtagaacga agcggcgtcg aagcctgtaa agcggcggtg
     4261 cacaatcttc tcgcgcaacg cgtcagtggg ctgatcatta actatccgct ggatgaccag
     4321 gatgccattg ctgtggaagc tgcctgcact aatgttccgg cgttatttct tgatgtctct
     4381 gaccagacac ccatcaacag tattattttc tcccatgaag acggtacgcg actgggcgtg
     4441 gagcatctgg tcgcattggg tcaccagcaa atcgcgctgt tagcgggccc attaagttct
     4501 gtctcggcgc gtctgcgtct ggctggctgg cataaatatc tcactcgcaa tcaaattcag
     4561 ccgatagcgg aacgggaagg cgactggagt gccatgtccg gttttcaaca aaccatgcaa
     4621 atgctgaatg agggcatcgt tcccactgcg atgctggttg ccaacgatca gatggcgctg
     4681 ggcgcaatgc gcgccattac cgagtccggg ctgcgcgttg gtgcggatat ctcggtagtg
     4741 ggatacgacg ataccgaaga cagctcatgt tatatcccgc cgttaaccac catcaaacag
     4801 gattttcgcc tgctggggca aaccagcgtg gaccgcttgc tgcaactctc tcagggccag
     4861 gcggtgaagg gcaatcagct gttgcccgtc tcactggtga aaagaaaaac caccctggcg
     4921 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
     4981 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac
     5041 tcattaggca ccccaggctt tacacttt
//
LOCUS       DAJTZL010000226         5050 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.227, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000226 DAJTZL010000000
VERSION     DAJTZL010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5050
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.227"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..348)
                     /locus_tag="OO544_003801"
     CDS             complement(<1..348)
                     /locus_tag="OO544_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HCR6769293.1"
                     /translation="MFDGELSFALKLAREMGRPDWRAMLAGMSSTEYADWHRFYSTHY
                     FHDVLLDMHFSGLTYTVLSLFFSDPEMHPLDFSLLNRREADEEPEDDVLMQKAAGLAG
                     GVRFGPDGNEVIPA"
     gene            complement(330..752)
                     /locus_tag="OO544_003802"
     CDS             complement(330..752)
                     /locus_tag="OO544_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HCR6769294.1"
                     /translation="MFLKTESFEHNGVTVTLSELSALQRIEHLDLMKRQAEQAESDSN
                     RKFTVEDAIRTGAFVVAMSLWHNHPKKTQMPSMNEAVKQIEQEVLTTWPTEAISHAEN
                     VVYRLSGMYEFVVNDAPEQAEDAGPAEPVSAGKCSTVS"
     gene            complement(768..1508)
                     /locus_tag="OO544_003803"
     CDS             complement(768..1508)
                     /locus_tag="OO544_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6769295.1"
                     /translation="MPVPNPTMPVKGAGTTLWVYKGSGDPYANPLSDVDWSRLAKVKD
                     LTPGELTAESYDDSYLDDEDADWTATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTA
                     TTGMTVTPASASVVKGQSTTLTVAFQPDGATDKSFRAVSADKTKATVSVSGMTITVKG
                     VAAGKVNIPVVSGNGEFAAVAEINVTAS"
     gene            complement(1516..1911)
                     /locus_tag="OO544_003804"
     CDS             complement(1516..1911)
                     /locus_tag="OO544_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6769296.1"
                     /translation="MKHTELRAAVLDALEKHDTGATLFDGRPAVFDEEDFPAIAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDSWMESRIYPVMSDIPALSDLITSM
                     VASGYDYRRDDDAGLWSSADLTYVIIYEM"
     gene            complement(1908..2486)
                     /locus_tag="OO544_003805"
     CDS             complement(1908..2486)
                     /locus_tag="OO544_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6769297.1"
                     /translation="MAIKGLEQAVENLSRISKTAVPGAAAMAINRVASSAISQSASQV
                     ARETKVRRKLVKERARLKRATVKNPQARIKVNRGDLPVIKLGNARVVLSRRRRHKKGQ
                     RSSLKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            complement(2498..2851)
                     /locus_tag="OO544_003806"
     CDS             complement(2498..2851)
                     /locus_tag="OO544_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HCR6769298.1"
                     /translation="MADFDNLFDAAIARADETIRGYMGTSATMTSGEQSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDEVRQLRRGDTLTIGEENFWVDRVSPDDGGSCH
                     LWLGRGVPPAVNRRR"
     gene            complement(2863..3261)
                     /locus_tag="OO544_003807"
     CDS             complement(2863..3261)
                     /locus_tag="OO544_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HCR6769299.1"
                     /translation="MTKDELIARLQELGEQLNRDVSLTGTKEELALRVAELEEELDDT
                     DETAGQDTPLSRENVLTGHENEVVSAQPDTVIQDTAELVTVVALVTLHTDALHATRDK
                     PVAFVLPGTAFRVSAGVAAEMTERGLARMQ"
     gene            complement(3303..4328)
                     /locus_tag="OO544_003808"
     CDS             complement(3303..4328)
                     /locus_tag="OO544_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HCR6769300.1"
                     /translation="MSMYTTAQLLAANEQKFKFDPLFLRLFFRESYPFTTEKVYLSQI
                     PGLVNMALYVSPIVSGEVIRSRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLA
                     DPAYRRRRIIMQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSAAN
                     NITQSGVTEWSKRDKSTYDPTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVKEKL
                     DTRRGSHSELETAVKDLGKAVSYKGMYGDVAIVVYSGQYVENGVKKNFLPDNTMVLGN
                     TQARGLRTYGCIQDADAQREGINASARYPKNWVTTGDPAREFTMIQSAPLMLLADPDE
                     FVSVQLA"
     gene            complement(4384..4716)
                     /locus_tag="OO544_003809"
     CDS             complement(4384..4716)
                     /locus_tag="OO544_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HCR6769301.1"
                     /translation="MTSKETFTHYQPLGNSDPAHTATAPGGLSAKAPAMTPLMLDTST
                     RKLVAWDGTTDGAAVGILAVAADQTSTTLTFYKSGTFRYEDVLWPEAASDETKKRTVF
                     AGTAISIV"
     gene            complement(4726..>5050)
                     /locus_tag="OO544_003810"
     CDS             complement(4726..>5050)
                     /locus_tag="OO544_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6769302.1"
                     /translation="ATEGENASAAQPDVNAQITAAVAAENSRIMGILNCEEAHGREEQ
                     ARVLAETPGMTVETARRILAAAPQSAQTRSDTALDRLMQGAPAPLAAGNPASDAVNDL
                     LNTPV"
BASE COUNT         1057 a         1553 c         1304 g         1136 t
ORIGIN      
        1 agcggggata acttcattcc cgtccgggcc aaagcggaca cctccggcaa gccctgccgc
       61 tttctgcatc agcacatcat cttcaggctc ttcgtcagcc tcgcgccggt tcagcagact
      121 gaaatccagc ggatgcatct ccggatcgct gaaaaacagg ctgagcacgg tgtacgtcag
      181 cccggaaaag tgcatatcca gcagaacatc atgaaaataa tgggtactgt aaaagcggtg
      241 ccagtcggca tactccgtgg atgacatccc ggcaagcatg gcgcgccagt cgggtcgccc
      301 catctcacgc gccagtttca gggcaaaact cagctcaccg tcgaacactt tcccgcagaa
      361 acaggctctg caggcccggc gtcctctgcc tgttcaggag catcattcac cacaaactca
      421 tacataccgg acagccggta caccacgttt tcagcatgag aaattgcctc tgtgggccag
      481 gtggtaagca cttcctgctc aatctgttta acggcttcat tcatggacgg catctgcgtc
      541 ttcttcggat ggttatgcca cagggacatc gcaaccacaa aagcgccggt tctgatggcg
      601 tcttccacag taaacttccg gttgctgtct gactccgcct gttctgcctg ccgtttcatc
      661 aggtcgagat gctcaatacg ctgcagggct gacagttcag aaagcgtgac ggtcacaccg
      721 ttatgttcaa atgattcggt tttcaggaac atcgctgact ctccggatta actggcggtg
      781 acgttgattt ctgcaaccgc agcaaactca ccattaccag atacgaccgg aatgttgacc
      841 ttgcctgcag caacaccttt cacggtgatg gtcataccac tgaccgacac ggtggctttt
      901 gttttatccg cagacaccgc acggaagctc ttgtcggttg cgccatccgg ctggaatgcc
      961 acggtcagcg tggtgctctg ccctttcact acggaagcac tggcgggtgt caccgtcatg
     1021 ccggtcgtcg ccgtcaccgt gctgcgatct tctgccatcg acggacgtcc cacattggtg
     1081 accttcaccg tgcgggtaat cacctccttc gccgtcaccg ccttaccgat actgctgacc
     1141 cagccacgga acacatcgac cgtgccgttc gggaagcgga ttttataggc acgggtatca
     1201 ccttcattaa accacgccag cagcgcctgc tgcccctgct ctccgggcat ccacgccagc
     1261 gtgaagctgg tatctccggc agatttctgc ccctgcccgg tcgcagtcca gtctgcatct
     1321 tcatcatcga gatagctgtc gtcataggac tcagcggtca gttcgccggg cgtcaggtct
     1381 ttaacttttg ccagacgcga ccagtcaacg tctgaaagcg ggttcgcata agggtcaccg
     1441 ctccccttat aaacccacag ggtggtcccg gcacctttca ccggcattgt aggatttggt
     1501 acaggcatag cgtcctcaca tttcatagat aatgacataa gtcagatcgg ctgaactcca
     1561 caggcccgca tcatcgtcgc gccggtagtc atagccgctg gccaccatac tggtgatcaa
     1621 atctgacagt gccgggatat cgctcatcac aggataaatc cgggactcca tccacgaatc
     1681 cagctctgaa tccggcacct gagcaggcag gaaaacttca atatgcagct ccgcctgcca
     1741 ggtatcgctg tccagctctt cgcccgtgta ttcagcgccg gtgagataaa cggcaattgc
     1801 cggaaaatct tcctcatcaa aaacagcggg gcgaccatca aaaagcgtcg ccccggtgtc
     1861 atgcttctcc agtgcatcca gtacggctgc acggagttca gtatgtttca tcgctttatt
     1921 accattctca gttgatgctg cagcgcatag cccagctctt tcggaagacg ttcacgccgt
     1981 atccgttcaa tattctgttt aaacgccgtg gtcagcggca ccgccatcgg gattttcacc
     2041 acatcaatgg ggtaacggtt tttcccggcc acacgctgca taacatgcca ccggccgttt
     2101 ttcagttgct gaataaacgc gccgggaata cgacggttac ccaccacaag cacgctgccg
     2161 ccacctttca gggatgaacg ctgccccttt ttatgacgcc tgcggcggga caggacaacc
     2221 cgcgcattac ccagcttgat tacgggcaaa tccccccggt taactttgat tctggcctgc
     2281 ggatttttga ccgtggccct tttcagcctg gccctttcct ttaccagttt ccggcgtacc
     2341 tttgtctcac gggcaacctg tgacgcagac tgcgatatcg cggatgaagc aacgcggtta
     2401 atggccattg cggcggcacc gggcaccgcc gttttgctga tacggctgag gttttcaacg
     2461 gcctgctcaa gaccttttat ggccatacat ccccctttca gcggcgacgg ttaacggcag
     2521 gcggtacgcc ccgtccaagc cagagatgac aacttccgcc atcatccggc gaaacccgat
     2581 ctacccagaa attttcctca ccgatggtca gcgtgtctcc acgccgcagc tgccgcacct
     2641 catcagtccg gacaaacagg gacgggctgg agccttcaac gcgcacgccc tgtccggcat
     2701 agctgatatt ttcagggtca tcaaaaacac cacgtatcac cgcacctgac tgctcaccgg
     2761 atgtcatggt ggctgacgtt cccatgtacc cgcgtatcgt ttcatcggcg cgggcaatgg
     2821 cagcatcgaa caggttatcg aaatcagcca cagcgcctcc cgttattgca ttctggccag
     2881 gccgcgctct gtcatttcgg ctgccacacc ggcagagaca cgaaacgccg ttcccggcag
     2941 cacaaatgcc acaggtttat cccgcgtggc gtgaagtgca tcagtatgca gcgtcaccag
     3001 tgccacgacc gtgaccagtt cagccgtatc ctgaatcacg gtatccggct gcgctgatac
     3061 cacctcattt tcatgcccgg tcagcacatt ttcccggctg agaggggtgt cctgaccggc
     3121 agtttcatcc gtgtcatcaa gctcttcttc cagctctgcc acacgaagcg ccagttcttc
     3181 tttcgttccc gtcaaactga catcgcggtt caattgctca cccagctcct gaagacgggc
     3241 aatcagttca tctttcgtca tgaactcctc cacagagaga aaatggcccc gaagggccat
     3301 gattacgcca gttgaacgga cacgaactca tcaggatcag ccagcagcat cagcggtgct
     3361 gactgaatca tggtgaactc acgcgccgga tcgccggtgg tcacccagtt tttcgggtaa
     3421 cgggcagaag cgttaatgcc ttcgcgctgt gcgtccgcat cctgaatgca gccataggtg
     3481 cgcagaccgc gagcctgagt gttccccagc accatcgtgt tgtccggcag gaagttcttt
     3541 ttgacgccgt tttccacgta ctgtccggaa tacacgacga tggccacatc gccatacatt
     3601 cccttatagg acaccgcttt gcccaggtct tttaccgctg tctccagctc ggaatgagag
     3661 ccgcgacggg tatccagctt ctccttgacg gctttgaagg aacggaacag cgcccagcct
     3721 ttcgggtcaa acacgatgat attcaccaca ccgctggcgt tcagcgcgta ggcttcgata
     3781 tcgtcggtcg ggtcatacgt ggacttgtca cgcttgctcc actccgtgac gccggactgt
     3841 gtgatgttgt tcgccgcact gcggcccata tccacctcaa ccggatcgaa ggcttcaccg
     3901 gtcatggtgt atttgccctt gagcacggca gaaactgcct gcatctcttc gacctgagca
     3961 atggccagct cttcgtcacg catgttctgc atgatgatgc gacggcggcg gtaagccggg
     4021 tccgccagat tctgcggatc ttcatccggc aggcgacgca gggtcatctg cggattcacc
     4081 tcatgcttgg gtttgacata tcccggcgta aattcagagg tggagccgcc acgggaacgg
     4141 ataacctcac cggaaacaat cggcgaaacg tacagcgcca tgtttaccag tcccggaatt
     4201 tgtgagagat agactttctc cgtagtgaag ggatagctct cacggaaaaa gagacgcaga
     4261 aacagcggat caaacttaaa tttctgctca tttgccgcca gcagctgggc ggttgtgtac
     4321 atcgacataa aaaaatcccg taaaaaaagc cacacaggcg gcctttagtg atgaagggta
     4381 aagttaaacg atgctgattg ccgttccggc aaacacggtc cgttttttcg tctcgtcgct
     4441 ggcagcctcc ggccagagca catcctcata acggaacgtg ccggacttgt agaacgtcag
     4501 tgtggtgctg gtctggtcag cagcaaccgc aagaatgcca acggcagcac cgtcggtggt
     4561 gccatcccac gcaaccagct tacgggtgga ggtgtccagc atcagcgggg tcattgcagg
     4621 cgctttcgca ctcaatccgc cgggcgcggt tgcggtatga gccgggtcac tattgcccag
     4681 cggctggtaa tgggtaaagg tttctttgct cgtcataaac atcccttaca ctggtgtgtt
     4741 cagcaaatcg ttaacggcat cagatgccgg gttacctgca gccagcggtg ccggtgcccc
     4801 ctgcatcaga cgatccagcg cagtgtcact gcgcgtctgt gcactctgtg gtgctgcggc
     4861 cagaatacgg cgggccgttt ccacggtcat accgggggtt tcggccagca cgcgtgcctg
     4921 ttcttcgcgt ccgtgagcct cctcacagtt gaggatcccc ataatgcgac tgttttctgc
     4981 cgcaaccgct gcggtgatct gcgcgttcac gtccggctgc gccgcgctgg cgttctcgcc
     5041 ctccgtcgct
//
LOCUS       DAJTZL010000227         5044 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.228, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000227 DAJTZL010000000
VERSION     DAJTZL010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5044
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.228"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            73..1601
                     /gene="lsrA"
                     /locus_tag="OO544_003811"
                     /pseudo
     CDS             73..1601
                     /gene="lsrA"
                     /locus_tag="OO544_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416030.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
     gene            1595..1903
                     /locus_tag="OO544_003812"
     CDS             1595..1903
                     /locus_tag="OO544_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000911157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6769303.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLMRKVVSAMISWIILSRARQKYGAASVVVDEQSWLSSGVKKSPY
                     "
     gene            complement(2075..3523)
                     /gene="uxaB"
                     /locus_tag="OO544_003813"
     CDS             complement(2075..3523)
                     /gene="uxaB"
                     /locus_tag="OO544_003813"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HCR6769304.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAISYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFAL
                     AALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDHVIGTQELVAIVLAEKDHWEQ
                     DLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(3750..4700)
                     /locus_tag="OO544_003814"
     CDS             complement(3750..4700)
                     /locus_tag="OO544_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HCR6769305.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGGFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPLTVMLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGL
                     VARMGGEEFAVAVPSVNPVDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRAS
                     YRTLTDDFNKLMVEADTCLYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(4839..>5044)
                     /locus_tag="OO544_003815"
     CDS             complement(4839..>5044)
                     /locus_tag="OO544_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4186 family protein"
                     /protein_id="HCR6769306.1"
                     /translation="ALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSL
                     SEEQQRYIVAVIYHWLVVQMNQP"
BASE COUNT         1316 a         1288 c         1229 g         1211 t
ORIGIN      
        1 taaggttatg aacaaattaa aagcagaaat acatttgttc aaaactcacc tgcaaaactg
       61 aacgggggaa atatgcaaac gagtgatacc cgcgcgttac cgctactttg cgcccgctcg
      121 gtttataaac agtattcagg ggtcaatgtc ctgtcgattt tacgttgcat caaggggagg
      181 tccacgccct gctcggcggc aatggtgccg gtaaatcgac gttaatgaag attattgccg
      241 gtattacccc tgctgatagc ggtacgctgg agattggggg caacaactac gtcagattaa
      301 cgccagttca tgctcatcag ctgggtattt atctcgttcc ccaggaaccg ctgcttttcc
      361 caagcctgtc gataaaagaa aacatcctgt ttgggctggc aaaaaaacag ctctccatgc
      421 agaaaatgaa gaacttgctg gcggcgctgg gctgctagtt tgatctgcat agtctggcag
      481 gatcgctgga tgtcgccgat cgccaaatgg tggaaatcct ccgcggcctg atgcgcgact
      541 cgcggattct gatcctcgat gaacctaccg cctcgcttac ccctgcggaa accgaacgct
      601 tgtttcgtcg cttgcaagag ctgcttgcta ctggcgtggg tattgttttt atctcgcata
      661 agctgccgga aattcgccag attgccgatc gaattagcgt gatgcgcgac ggaaccatcg
      721 ccttaagcgg caaaaccagc gaactgtcta ccgatgacat tattcaggcc atcacgccag
      781 tggtacggga aaaatcgctc tctgccagcc aaaaattatg gctggagtta cctggcaacc
      841 gcccacaaca tgccgccgga acgtcggtgc tgacactaga aaatctgacc ggcgaaggtt
      901 tcaggaatgt cagcctgacg ctcaatgccg gagaaattct gggtctggcc gggctggtgg
      961 gggccggacg cacagaactg gccgagacgc tctatggtct gcgtactttg cgtggcggac
     1021 gcattatgct gaatggtaaa gagatcaata aattatccac tggagaacgt ttactgcgcg
     1081 gtctggttta tctgccggaa gatcgccagt catccggact gaatctcgat gcttcgctgg
     1141 cctggaacgt ctgcgccctt actcataacc ttcgtggatt ctgggcgaaa accgcgaaag
     1201 ataatgccac cctggaacgt tatcgtcggg cgctgaatat taaatttaac caaccggaac
     1261 aagctgcacg gacattatcc ggtggcaacc agcaaaaaat cctgattgcc aaatgcctgg
     1321 aagcttcgcc gcaagcattg attgtcgatg agccgacgcg cggcgtggat gtctcggccc
     1381 gtaatgatat ctaccagctg ttgcgcagca tcgccgcaca gaatgtggca gtgctgctta
     1441 tctcctctga tctggaagag atcgaactga tggcagatcg tgtgtatgtg atgcatcagg
     1501 gcgaaattac ccactctgca ctgaccgggc gcgatattaa tgtcgagact attatgcgcg
     1561 ttgccttcgg cgatagtcag cgtcaggagg cgtcatgctg aagtttattc agaacaaccg
     1621 tgaaatcacg gcactgctgg cggtggtgct gctgtttgta ttacccggtt ttctcgaccg
     1681 ccagtattta agtgtgcaaa cgctgaccat ggtttatagc agcgcgcaaa tcctgatcct
     1741 gctggcaatg ggcgcgacgc tggtaatgct tatgcgcaaa gtagtaagcg cgatgatatc
     1801 ttggataata ctgagccgtg ccagacaaaa atacggcgca gccagtgttg ttgtggacga
     1861 acaatcatgg ttatcttcag gcgtgaaaaa gtcgccctat taaacgtgat tacgatcaca
     1921 ttctcaagac gctacttaca aattacctac aagcttacaa cattaacagg cgctgcatgt
     1981 ggcatctgtt aatgataaag gcacatagga tgttgtaact aatatagtga aataagaaac
     2041 ccggtcaaaa ccgggttcag aagtaacggt gttattagca caacggacgt acagcttcgc
     2101 gcatcccttt ttcaagaatc gcatccaggt cattagcaac ctgctcgacc agaccaggca
     2161 cttgcgtcag gtcctgctcc cagtggtctt tttctgccaa cacaatcgct accagttcct
     2221 gggtaccgat aacatggtca cgatgctggc tccagagttg ctgataacgt tccagccagt
     2281 gtgcatcgtc ctgtaccgga tatgtttctc cgttacgctc accacgatag aacgcaatca
     2341 atgccgctaa tgcgaaagta aggcgcgccg gaagtgtgcc gtttgccttc tgccctgcca
     2401 gcagctgcgg caggatgcgg gtgcggaact tggtcatacc gttgagcgcg atagacagca
     2461 gctgatgctt atacgggtta cggaaacgcc cggtgactgc actggcgaaa gattccagtt
     2521 catcacgagg caaatccagt accggaataa tttcttcgta gatggctttt tcaacgaatg
     2581 cgcaaatttc agcatcgttc atcgcttcac ctacggtatc cagccctgcc tggaacgcca
     2641 ccggcaccag cgcggtgtgc gcaccgttga ggattgccac tttgcgttct ttatacggtt
     2701 taatatcgtc aacaatcagc acgttgagcg gatatttgtc caggcgcagc tcagtcgcta
     2761 aggatttcgg cccctgaatc acaaacaggt aaaagtgttc agcggtgtcg aggaaaccat
     2821 cgtgataacc cagttcttct tccagtttag ccacttcatc gcgcggataa ccggtaacga
     2881 tacggtccac cagcgtagaa cagaagctgt tagcctgatc cagccattga ataaatgctt
     2941 ctggcagtgc ccactcttgc gcatagcgca gcaccagttc acgcaacgcg tcgccattgt
     3001 agtcgatcaa ctcacacggg atgatgatcc aacctttatc cagcgcaccg ttgaaatggc
     3061 taaagcgttc gaacagcaga cgggtcagtt ttgccggata gcttatcgct ggcgcgtcat
     3121 cgaatttatc gcccgcatgg tagctgatcc ccgcttcggt ggtgttagag aaaacaaagc
     3181 gcatttccgg gttgtgcgcc agtttcagga attcatcgta ttcactgtag acgctgattt
     3241 cacgattaac cgagcgaatc agacgcgcgt cgctgaccgc ttcccccttc tcattcaggc
     3301 cacggataat ggtggtatac agaccatcct gcgtgctcag tgacggcggg aatgaagttt
     3361 caatcggacg aacaacgacc acgccagaat tcagatcggt gtgctcattc aggagatcga
     3421 tttgccagtc aacaaaggcg cgcaggaagt taccttcacc aaactgaatg atacgttctg
     3481 gatactgtgc accgggaaaa tcgcgacgat ttagtgtttt cacaatgggt tcccttctga
     3541 ttagtcatac aacctgtttt aattggtacg acaggttagc aaactttaat acgccgaacc
     3601 cctgttttga tcaactcctg atgattaatg agcagtttta tgagaaaagt gtggcgcgga
     3661 tcatggttta atcgaggaaa tacgcctttt cctggatcat aaagtggtag aacacattgc
     3721 attcaaatcg cgcggaatga ataaagatgt cagacaactt cctcaccgta acgcatagtg
     3781 ctggtacggt tgcgcccatc tttcttcgag cgatacagac acgtatcggc ttcgaccatc
     3841 aatttattaa aatcatcggt cagcgttcgg tacgatgcgc gaccactacc gacgccaata
     3901 cttaccgtga gataaagcgt tttttgttgc caggtgaatg gttgcagttc aacgccttta
     3961 cggatttttt ccgccattag cagaccatcg acaggattca ccgacggcac tgcaacagca
     4021 aattcttcgc cgcccatgcg cgccaccagc cccttatcac cgacaatctt ctgaatatgc
     4081 cgggcaaaca cgcttaacac tttatcgcca cattcatgtc catagttatc gttaatgctt
     4141 ttgaaatagt cgatatcaag cagcatgacc gtcagaggca cgatcaacac gcccgttgag
     4201 aattgctcac tgaaccagtc cgcaagcaaa ggccagaaat ccagactatc aatactgacc
     4261 gaaccaatcg ccccgacaat agcgcataat aatgcggcaa tcagacagta attaaatagc
     4321 cgtaaggcac ttactggctc atacttattt ttccccagac gcttatcccg cacgaccagt
     4381 aaggcgacgg taacaataaa catcatattg gagaaattga taaccagtga aaccagtccc
     4441 cattcggtgg ttatggcatc ataaaccagc atcgccacat aactgatcgc atagtaatgc
     4501 agtcgattaa gccagacata gcgggcaaac ccccccgcca ttacgccatt cagcggccag
     4561 aaaagagaaa gttcgtgaac cagccgtaac tctgcaccaa taaaatagaa cagcgtggtt
     4621 agcgcaaaaa tagcgatgct attacgtagc agatgacctt cctggaacaa acggaaagtt
     4681 gaaatggggt gtacatgcat taattatctg tccatacaat tgcgggtggc aattatctca
     4741 acgtaataag taaattctca taaaggatag catgttcgct aacagcggcg gcaaataaag
     4801 gtaccctggg aatgctattt tatcgttttc cgcaattctc atggctgatt catctgtaca
     4861 accagccagt gatagattac cgccacgata taacgttgtt gttcttcgct taacgaaaca
     4921 ccctgcggaa tgttatgcca cttagccaga caaccgcgac agcaggtcgc ggtggcgtgc
     4981 tgagcgataa acaccggatg cccgcgcatg ggggtttgct taccgtcatt agccggtaat
     5041 gccg
//
LOCUS       DAJTZL010000228         4838 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.229, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000228 DAJTZL010000000
VERSION     DAJTZL010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4838
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.229"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(263..463)
                     /locus_tag="OO544_003816"
     CDS             complement(263..463)
                     /locus_tag="OO544_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6769307.1"
                     /translation="MLCIKFEYLTDKMIKHVSDLLIKEGGFGDACNPKDIFIHATSPN
                     ATLKTAVTAEWFERNKAELGYW"
     gene            complement(463..735)
                     /locus_tag="OO544_003817"
     CDS             complement(463..735)
                     /locus_tag="OO544_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000816065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR6769308.1"
                     /translation="MKQWRDDIKRFFATYFMINYETGMLEWIDGGEVKTRDDAYGNPM
                     IRYGTRDYYLKYVIWFLHHEVQATKKIIFRDGNKRNCHPNNLLLEI"
     gene            complement(738..1016)
                     /locus_tag="OO544_003818"
     CDS             complement(738..1016)
                     /locus_tag="OO544_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6769309.1"
                     /translation="MNKHTLTRVTKTAFDELNRIKNITGLPHSAVMKLAINRDINEQD
                     LLNSRLEAARHHIRIKQDDLDSLKSLNAFGLDLARLLSIKIHKLSQEV"
     gene            complement(1017..1574)
                     /locus_tag="OO544_003819"
     CDS             complement(1017..1574)
                     /locus_tag="OO544_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6769310.1"
                     /translation="MQYSEFRAMLQASRERNKHNSYAYTNNPTSYEVPTFSKSERKNI
                     EAVIRSITPRDRFMPVRKTTKNTIKNYLANFDSYEQLPGKLDDVFIGFCRSEGHPKYN
                     KKLFYLLKNLDEINASTVTNHLQRQATRLSYELPSDKYCALLAVMCAKLIGIVEHHIA
                     VGNIEPMENEQPDFEFDPYLVTEDF"
     gene            complement(3138..3689)
                     /locus_tag="OO544_003820"
     CDS             complement(3138..3689)
                     /locus_tag="OO544_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR6769311.1"
                     /translation="MAILGYCRVSTDDQSITNQQMQIEEAGYNIAKWFTDEAVSGSVK
                     ASLRNGFSRLLAYAREGDTVVVVAVDRLGRDTIDVLSTVKALQAKGVTVISLREGFDL
                     SSAMGEAMLGIMSTLAQLERSLIAERRKAGIERAKAEGVHMGRPVKASSEAVQTLISQ
                     GKTRLQIQEELGISRATYYRLAK"
     gene            4488..4784
                     /locus_tag="OO544_003821"
     CDS             4488..4784
                     /locus_tag="OO544_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HCR6769312.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
BASE COUNT         1362 a          994 c          923 g         1559 t
ORIGIN      
        1 aattgataca ccggaatcag cgaaagcctg caccatcata gcagtgtcgc tatcaatgaa
       61 aatctgtaat tcatattcgt tcatttttaa aatctccttt ttaccagacc tcctcaatcg
      121 ctctgtaacg agctaaacag tctttttcag gggtagcctg tacagtggta ttacccaccc
      181 taaaataaga tagttttttg cgttagaatg cgattgagag cgtctgataa gctgtttttc
      241 taatttctct taaagtctcg gactaccaat aacctaattc cgctttattt cgttcaaacc
      301 attccgctgt aactgctgtc tttagtgttg cgtttgggct ggtggcatga atgaagatat
      361 cttttggatt gcaagcatca ccgaaaccac cttctttgat gagcaggtct gatacatgct
      421 taatcatttt atctgtcaaa tattcgaatt taatacataa cattaaatct ccaggagtaa
      481 attgtttggg tggcaatttc ttttattacc atccctgaag ataatctttt tagttgcctg
      541 aacttcatga tgcaaaaacc agatgacata cttcagataa taatcacgag tgccataacg
      601 aatcattgga ttaccgtaag catcatctct tgtcttaacc tctccaccat caatccattc
      661 aagcattcct gtctcataat taatcatgaa gtaagttgca aagaaccgtt taatatcatc
      721 tcgccattgc ttcataatca gacctcctga gacagtttgt gaattttgat acttagtaac
      781 cttgctaaat ccagtccaaa agcatttaag gatttcagac tatcaaggtc atcttgctta
      841 atcctgatat ggtgtcttgc agcctctaag cgactattaa gcaaatcctg ttcgttaata
      901 tcacggttaa ttgcaagctt catcactgct gagtgtggca gacctgtaat gtttttgatt
      961 cggttcagtt catcaaaggc tgtctttgta actctggtta atgtgtgttt attcatttaa
     1021 aaatcctctg taactaaata ggggtcaaac tcgaaatctg gttgctcgtt ttccattggc
     1081 tcaatattcc caacggcaat atgatgttca acaatgccaa ttaatttcgc gcacatcaca
     1141 gcaagcaaag cgcaatattt atcgcttggt aattcgtaac taagtctggt tgcctgtcgc
     1201 tgaagatgat ttgtcacggt ggatgcgtta atttcgtcaa gattctttag cagataaaat
     1261 agttttttgt tgtatttcgg gtggccttca cttctacaga acccaataaa aacatcatca
     1321 agcttgcctg gtaactgctc gtaactgtca aagttagcca gataattttt aatagtattc
     1381 tttgttgttt tgcgtactgg cataaatctg tctcgtggcg taatgctacg aatgaccgct
     1441 tcaatgttct tacgttcgga tttgctaaag gttgggactt cgtagctggt ggggttgttt
     1501 gtgtaagcgt aggagttgtg cttgttgcgt tctcttgatg cctgaagcat cgctctaaat
     1561 tctgaatact gcattcatgt tctgactctc ataagatgtt attattctgt taacctatat
     1621 acaatattag cattttcaaa gactaatgtc aagtattttt aacattttcg taacatctgt
     1681 ggtataatgg gtttatgggg agagtaaaat atgaggcgtg gctatgtatt tggtcgcata
     1741 ttttgcattg cacacagacc tcttcaatcg ccctgtaacg agttaaacga cctcttcagg
     1801 tcaactcata aggtcaggta ctttttgtgt cagaatgcga ttgagagcgt cttacaagga
     1861 aaactaatgg taaatctccc tcctgagctg gggtcacctg atgtatggac tggtggaggt
     1921 attgggctga ttgcaggcct gctacttcgc tacgtcctcg tcagagtgct tgttgtcttt
     1981 aaactgttcg gtcgtacatg gacgcttgac cttaaggata acgaccacac agacccccgt
     2041 taaactcctt caaaacccct gcacgagatg acagatagaa gtctatggta caggggtgtc
     2101 tttagcattt tgtgctatct attaaaaaaa tagcttgaca gaaccggaaa gccgtatcta
     2161 tcaagcattc acctctctga cagtaatttc taaaagttct tatttttcaa taagttgtaa
     2221 ttagccatat atagagaact aaaagatttt ttagctattt tgtttaaaag catatcaaac
     2281 gatacatttt attgatttag gtcaaagaca aaagcattga caaaatggta agattgttca
     2341 catgacctta cttaacagac ttttaagaga ctttgaaaca gcctgtttaa caggtcacta
     2401 cctctggtct tatcgttccc ttctttctag gtggttatct gttaagagtt tttcagtttt
     2461 tattcttaag ataattgtta agatgatact ctcccttcgg gttatatcgt atctcttctc
     2521 ccgttaaata tcccttcaca gtcagactaa ataacacctg tgtagttctt ggtagaatct
     2581 ttcaaggtaa cttagctctg agtttatgaa gttgacctat gagatagact ctaccgaatc
     2641 tgttagtcaa ttgttgctta ggtgctgcta aggcttactt agtggaaaga gaggggatat
     2701 gtgaccatag gggaacccct cctgttaaaa gactacttag aagtctgagg ttttagtaaa
     2761 atctgtgaat atggcttctc agacgctctg taacgcattc taagcgattt taccccttta
     2821 ttttaaggtg gttagtacat ccctataggt tacccctaaa aatggggtta aaactcgcta
     2881 cagagctttc tatgagatta gcttctaatc gaatcaacta tgaggtttgg taggattgtg
     2941 caaaatctgt acagtaactt ttcagaatct gaaaattttt ataaaaaatg actagccctc
     3001 tttgaatctc atcttccccg aatcttcaaa aatatctgac agatgtctgt agctgctaag
     3061 tacccgtcac accagctatg tgaccccctt ggcaaaaaag ccaaatatca gctacatcca
     3121 tgtaactatt cagtaactta tttagcaagt ctgtaataag ttgctctaga gattcctaac
     3181 tcttcctgaa tctgcaacct cgtcttacct tgtgaaataa gcgtctgtac agcctctgaa
     3241 gaagctttta caggcctccc catatgtacc ccttcagctt tagcacgttc aataccagcc
     3301 tttctacgct ctgctatcaa gcttctttca agctgtgcca acgttgacat aatgcctaac
     3361 attgcttcac ccatagcgct tgataagtca aacccttctc taaggctgat aactgtcaca
     3421 cctttagcct gtagcgcttt aaccgttgat aacacatcaa ttgtgtcacg tccaagacgg
     3481 tctacagcta ccacaactac ggtgtcacct tctcgtgcat aagctaatag tctgctgaaa
     3541 ccgtttctta aagaagcttt gacagaacca ctgacagctt catcagtgaa ccatttagca
     3601 atgttgtacc ctgcctcttc aatttgcatt tgctggttag tgatggattg gtcatctgta
     3661 ctcaccctgc aatatccaag tatcgccata atgttcccct tagaagtctc aaaagttagt
     3721 ctcataagtt tagctgtctc ataatccaaa aacaaccttt tgagactact tgagggtgca
     3781 ttctaccgtt acgttgaaag tgtctcaaaa ggaagtcctt ttaagactgg ttatgaagga
     3841 gctatgaggt tgattagggg ttggtacaag cccaactaac cgaatcaact atgaagattt
     3901 taggagggta actgtaacca tcactttcta actttcacgt aggaaattgt taagtggttt
     3961 gatagttaca ccatgtgacc aagcatctat atatcgttat atagccctgt atatagcact
     4021 atatgtagtg tcgagagagg aacgactaac acgatacagt ttaccctgaa aagagacttc
     4081 acagattctt tatatatttc aataagttag ggtaaaaagg gactataccg agactgtata
     4141 gaatctgtgt agaattgcat agatacttgg aaatctacta ttaaattgca tagggactca
     4201 cctgatattg tcctgttttt aacaggtctc ctagaaaacc gaatagactt cattcaaggg
     4261 tcattgaagt cataatcatt tcagtagaaa aaccaagtct cgattttaaa tgcaaatagc
     4321 aatcatattc aataaggcaa gacgatcagg atcagtgaaa atgtcatttt tggcgtaatt
     4381 gaccttactt tatgcatggt cttaacaaag acatcatctt tccgttgcag atcttcgccc
     4441 tttcaaataa cgtactttac aactttcccg aacaaggagt tgtgcccgtg ttgtatgtta
     4501 tttacgctca agacaaagct gatagcctcg aaaaacgcct ttccgttcgt ccggcacatt
     4561 tagcacgttt acagttactg catgatgaag gtcgtttgtt gacagctggt ccaatgccag
     4621 cggtagacag taacgatcca ggggctgcgg gatttactgg ctcaaccgtt attgctgaat
     4681 ttgaatcgct ggaagcagct caggcctggg ccgatgctga cccgtacgta gcggcaggtg
     4741 tctatgaaca cgtttcagtg aaaccattta agaaagtgtt ctgaatagag attcaatatt
     4801 taagggcacg gtttttgcaa ccgtgttctt gtttggcc
//
LOCUS       DAJTZL010000229         4836 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.230, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000229 DAJTZL010000000
VERSION     DAJTZL010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4836
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.230"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..187)
                     /locus_tag="OO544_003822"
     CDS             complement(<1..187)
                     /locus_tag="OO544_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6769313.1"
                     /translation="METVFDALKALKRASSHVVASRLGISREDAVNELWKLKRRGEAD
                     NKGSMWWLIQAGESEPVS"
     gene            complement(209..955)
                     /locus_tag="OO544_003823"
     CDS             complement(209..955)
                     /locus_tag="OO544_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR6769314.1"
                     /translation="MKNIAAAGVLERIHRLAPQGAVPPYRTVEEWREWQLAEGRKRSE
                     EINRQNRQLRVEKILNRSGIQPLHSKCSFANYQVQNDGQKYALSQAKSIADELMTGCT
                     NFVFSGKTGTGKNHLAAAMGNRLMAKGRSVIIVTESDVMSVLHDSYDNGKSGEKFLQE
                     LCGVDLLVLDEIGIQRETKNEQVVLHQIIDRRTASLCSVGMLTNLNHAAMSTLLGERI
                     MDRMTMNGCRWVTFNWDSWRSNVSFPGVVK"
     gene            complement(962..2026)
                     /locus_tag="OO544_003824"
     CDS             complement(962..2026)
                     /locus_tag="OO544_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer"
                     /protein_id="HCR6769315.1"
                     /translation="MANAWLRLWHDMPNDPKWRTIARVSGQPIATVMAVYIHLLVSAS
                     RNVTRGHIDVTTEDLASALDVTEEVIDSILQTMQGRVLDGDLITGWEKRQVLKEDNGN
                     ISQTAKSPAERKRAQRERERKREQNGDCHGASRNVTHMSRRVTTDTDKDTDKEDQNTM
                     VHGVKNATNQAGDVQTVNPGQPAGSTPEADSAYALKADSGAVQQVMTARPEQSLQLQQ
                     PEADSAIQREADRVAPENTGQPVGRVDYPDVFEQVWREYPLRAGANPKKSAFSAWKAR
                     LREGVPPETMLDGVRRYARYLAATGKAGTEFVQRATTFFGPDRNFENPWLLPVSGTNN
                     QRCVNHISEPDTEIPPGFRG"
     gene            complement(2098..2523)
                     /locus_tag="OO544_003825"
     CDS             complement(2098..2523)
                     /locus_tag="OO544_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCR6769316.1"
                     /translation="MKIKHEHIRMAMKAWAHPDGEKVPAAEITRVYFELGMTFPVLHN
                     DSKHNTLYLNTQKIFRWLDKDTPDAVEKIQALLPAIERAMPPPLVARMRSHSSAYFRE
                     LVETHERLVRDADDFVAVAIAGFNQMNRGGPAGNAVAVH"
     gene            complement(2520..2783)
                     /locus_tag="OO544_003826"
     CDS             complement(2520..2783)
                     /locus_tag="OO544_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6769317.1"
                     /translation="MNLGEYLHHFHITQSSFAEIVGASQGMVSHVITGRAKLTGEKIL
                     RWCEATGWVVTPHEIDSKTYPNPTDGLPAEFQANTQPSAGVDS"
     gene            2895..3395
                     /locus_tag="OO544_003827"
     CDS             2895..3395
                     /locus_tag="OO544_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6769318.1"
                     /translation="MKRKSLSDTDLNAARRLKEIWMAKKSQLGLTQERAAEIMGFSTQ
                     GAVSHYLNGQTPLNLEAVLKFAGLLKVPPESIRPDMVDLLQIARMYCREPQQDNIVAL
                     PADTEQTEDELPFAVEPMERDLIHTFRAFPKEDQEHMLQEMKDKKESMDRTVARWLAA
                     QKGRRA"
     gene            3415..3609
                     /locus_tag="OO544_003828"
     CDS             3415..3609
                     /locus_tag="OO544_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HCR6769319.1"
                     /translation="MSTVLSPMVSEFETVEQENSYNEWLRAKVAASLADPRPAIPHDE
                     VMAEMEQIIAQIAAATHRGE"
     gene            3609..3899
                     /locus_tag="OO544_003829"
     CDS             3609..3899
                     /locus_tag="OO544_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012601411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCR6769320.1"
                     /translation="MLPVLWLPSARDDLRQIVAYIAKENPAAARRMKIRIETSVLPLT
                     EHPYLYPPSERVLGLREIVTHPNYIILYRVTASSIEVVNIVHSRRQYPGKNS"
     gene            4180..4332
                     /locus_tag="OO544_003830"
     CDS             4180..4332
                     /locus_tag="OO544_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HCR6769321.1"
                     /translation="MDTIDLGNNESLVCGVFPNQDGTFTAMTYTKSKTFKTESGARCW
                     LARNTD"
     gene            4332..4709
                     /locus_tag="OO544_003831"
     CDS             4332..4709
                     /locus_tag="OO544_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001241301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6769322.1"
                     /translation="MRLTMEFKDLPSDVQKTAAHTLHSVLREIGKDIASEPAKDLARK
                     IKTAFVELYNVGTDSETVETKTVSSPIFSLEPSVLSGEICTEISSELLPVIREAIRRR
                     GLDGSYDHDVLQVLRTMVTSLGI"
BASE COUNT         1200 a         1279 c         1098 g         1259 t
ORIGIN      
        1 gtgacactgg ttcactttca ccagcctgaa tcagccacca catcgaaccc ttgttatccg
       61 cttcaccacg gcgcttcagt ttccacagtt cgttgaccgc atcttcacgg ctgattccaa
      121 ggcgggacgc caccacatgt gaagaggctc ttttcagtgc tttcagtgcg tcaaatacag
      181 tttccattaa aacatcctcc aacaaaaatt acttcacaac tcccggaaag ctgacatttg
      241 aacgccagct atcccagtta aacgtcaccc accgacaacc gttcatggtc atgcggtcca
      301 taatcctctc accaagaagc gtgctcattg cggcatgatt caggtttgtt aacatcccga
      361 cactgcacag tgatgctgtc cggcgatcaa ttatctggtg caataccacc tgctcgtttt
      421 tcgtctcccg ctgaatgcct atttcatcca ggaccagcaa atcaaccccg caaagctcct
      481 gtaaaaattt ttcaccggat ttgccgttgt cgtagctgtc atgcaacacg ctcatgacat
      541 cagactcggt gacgataatc acgctgcgcc ccttcgccat cagccggtta cccatcgccg
      601 ctgcaaggtg atttttcccg gtgccggttt taccgctgaa cacaaaattc gtgcacccgg
      661 tcatcagttc gtcagctatg gatttggcct ggctcagcgc gtatttttgc ccgtcgttct
      721 gcacctgata attcgcaaac gagcatttgc tgtgcagagg ctggatgccc gaacgattca
      781 ggattttttc cacccgcaac tggcgattct ggcggttaat ctcctcgctg cgttttcgtc
      841 cttcagcaag ttgccattcc cgccactcct ccaccgtccg gtacggtgga accgccccct
      901 gtggtgcaag tctgtgaata cgttcaagaa ccccggctgc cgcaatgttt ttcatgccac
      961 atcaccccct gaatcccggc ggaatttcgg tatccggttc agaaatatga ttcacacaac
     1021 gctggttgtt cgtgccgctt accgggagca accaggggtt ttcaaaattc cggtccggcc
     1081 caaaaaacgt cgtcgctcgc tgaacaaatt ccgttcccgc tttcccggtc gccgccaggt
     1141 atctcgcgta acgcctcaca ccatccagca tggtctctgg tggcaccccc tcgcgcaatc
     1201 tggccttcca ggcactgaat gcggatttct tcgggtttgc cccggcacgc aacgggtatt
     1261 cccgccagac ctgttcgaac acatccggat aatccactcg tcccacaggc tgcccggtgt
     1321 tttccggggc tacccgatcg gcttcccgct gaatggcgga atcggcttca ggctgctgca
     1381 gttggagtga ttgctccggc cttgcggtca tcacctgctg cacagcgccc gaatcggctt
     1441 tcagcgcata cgctgaatcg gcttccggtg tcgagcctgc tggctgacca ggattgacgg
     1501 tctgaacatc ccctgcctgg ttcgtggcgt tttttacgcc atggaccata gtgttttgat
     1561 cttctttatc tgtatcttta tctgtatctg tcgtgactcg tcgtgacatg tgcgtgacat
     1621 ttcgtgacgc gccgtgacaa tcgccatttt gttcccgctt tctttccctc tctcgctgcg
     1681 ccctcttgcg ctctgcagga gattttgcgg tttgcgaaat attgccgttg tcctctttaa
     1741 gcacctggcg tttttcccat ccagtgatta aatcaccatc aagtacccgc ccctgcatcg
     1801 tctgcaaaat tgaatcaatt acctcttctg tcacgtcgag cgcacttgcc aaatcttctg
     1861 tcgtgacatc aatgtgacct cgcgtgacat ttcgtgacgc gctcaccagg aggtggatat
     1921 acactgccat cactgttgca attggctgcc ctgacaccct ggcaattgtt cgccacttag
     1981 ggtcatttgg catgtcatgc cataatctga gccaggcgtt agccatactc acctcttctg
     2041 ataccgaatc tttttactca cgagttgccg ggagcgattc gatatggcta ttgtcagtca
     2101 atgtactgcc acagcatttc ctgccgggcc accacggttc atctgattga aaccggcgat
     2161 tgccactgcg acaaaatcat cagcgtctct caccagtcgt tcatgcgtct ccaccagttc
     2221 ccgaaaataa gctgaactgt ggctgcgcat tcgagccacc agcggaggtg gcattgccct
     2281 ttcgatcgca ggtaacaacg cctgaatttt ttcaacagcg tcaggggtgt ctttatccag
     2341 ccaacggaaa atcttttggg tgttgagata aagcgtgttg tgcttgctgt cgttatgcag
     2401 aaccggaaac gtcattccca gttcgaaata aaccctggtt atttcagctg ccggaacttt
     2461 ttcaccgtcc ggatgcgccc aggctttcat cgccatgcgg atgtgttcat gcttgatttt
     2521 catgaatcaa cccccgccga tggttgtgtg ttagcctgaa actcagcagg caagccgtca
     2581 gttgggttgg ggtaggtttt actgtcaatc tcatgtgggg tcaccaccca tcccgttgct
     2641 tcacaccaac gtaatatttt ttctcctgta agttttgcac gccccgttat aacgtggctg
     2701 accatccctt gggatgcgcc aacaatctca gcaaatgaac tctgggttat atgaaaatga
     2761 tgcaaatatt ctccaagatt cataatccgc cctatgtgat gtgttaccaa tgcataatta
     2821 atagcactgt tatttttaaa agtaaatagt gatactattt caaagtgatt agcaatctta
     2881 ttagaattgg atgtatgaaa agaaaatcat tgtcagacac cgacctcaac gccgccagaa
     2941 gactgaaaga aatctggatg gcaaagaaat ctcagttggg attgactcag gaacgtgcgg
     3001 cggaaattat ggggttttca acacaagggg cggtaagtca ctacctaaat ggtcaaaccc
     3061 cactaaacct tgaggctgtt ctaaagtttg ctggcttact gaaagttccc cctgagtcaa
     3121 tcaggccaga catggtagat ttgctgcaaa ttgcaaggat gtattgtcga gaaccacagc
     3181 aagataacat agtcgcatta ccagcagaca cagaacaaac agaggatgaa ctaccgtttg
     3241 ccgtagagcc gatggaacga gatttaatcc atacgttcag agcttttccg aaagaagatc
     3301 aagaacacat gcttcaggag atgaaagata aaaaagaatc aatggatcgt acggttgcgc
     3361 ggtggttagc tgcacaaaaa ggccgtcgcg cctaaatcag aggagatatg taaaatgagt
     3421 acagtccttt ccccaatggt ttcagaattt gagacagttg agcaagaaaa tagttacaac
     3481 gaatggctgc gggccaaagt agcagcaagc ctcgcggatc cccgcccggc gattccgcat
     3541 gatgaggtaa tggcggaaat ggaacaaatt attgcccaaa ttgctgctgc aactcacaga
     3601 ggtgaataat gttacccgtt ttatggctac catctgctcg tgatgatttg cgccagatcg
     3661 tagcctacat agctaaagaa aatcccgcgg cagcacgcag aatgaaaatt cgaattgaaa
     3721 catccgtttt gccgctcaca gagcatcctt acctgtatcc tccaagcgag cgagttcttg
     3781 gtcttcgtga aattgtaact catcctaact acatcatcct ttaccgagta acagcatcaa
     3841 gcatagaagt agtaaatatt gtgcattctc gaagacagta tccaggcaaa aacagttaat
     3901 tattcccgcc gacagaacca ccttagggtg gttttttctt gccccaacaa aaaacaatag
     3961 cgatgctatt tacatcaaaa aaaaataatg atgctattgt ttcaggcgtc aacccacccc
     4021 gccccacaga atgcagggca atacttcgag ttaccagaca gtggtcaggg gttaagtagc
     4081 cagcccgagg cgtaagaaca tgacggcagg gttcaatctt taactatgca gcaggttttt
     4141 tgttccgcca ccccggcgtt aaggggaaat gaggtcagca tggatactat cgatcttggc
     4201 aacaacgaat ctctggtgtg cggcgtgttc cctaaccagg atggaacgtt taccgccatg
     4261 acgtatacca aaagcaaaac gtttaaaacc gaatctggcg cgcgttgctg gttagccaga
     4321 aacactgact gatgaggctg acgatggaat ttaaagattt accgtctgat gtacagaaaa
     4381 cagcagctca tacattgcat tctgtgctgc gagaaatcgg gaaagatatt gcaagcgagc
     4441 cagcaaaaga tctggcccgg aaaatcaaga ccgctttcgt tgagctttat aatgttggca
     4501 ctgactctga aactgtcgag accaagaccg taagttcacc aatattctca cttgagccaa
     4561 gcgtattatc aggtgaaata tgcaccgaga tttccagcga actgcttccg gtaatacgag
     4621 aagcaattcg ccgtcgcggt ttggatggaa gttatgatca tgacgttctg caagttctca
     4681 ggacaatggt gacttcactg gggatttgat ccctgcgtcc tctaagcttt tgatatagcg
     4741 gtcctccaag aagcggtaaa ttttgtcgaa atcatctttt ccctctggtt tattcagagg
     4801 attacaggac ccgtaagtag cggcatacat ttcaac
//
LOCUS       DAJTZL010000230         4803 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.231, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000230 DAJTZL010000000
VERSION     DAJTZL010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4803
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.231"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(279..1322)
                     /locus_tag="OO544_003832"
     CDS             complement(279..1322)
                     /locus_tag="OO544_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCR6769323.1"
                     /translation="MNDRLCFEVHDNKGYFVFPDTWFGPLLGEFEEVLDAYDADEISE
                     TSYINKLRRLAQREPDFIDIHAHLAYAFLEQNAPRKALNASLKGLAAGNRIIPESFCG
                     EIIWMHPENRPYLRALYAAILANVHLQRHQDAVMLTDKILAYNPEDNQGARWLLGSEL
                     LRTGDHERAFSVLKEHADEFSPYWYELGLLHFLNGEHVKAATAFRHGFATNTYIAEML
                     CGNLHPFPLAVWHDFSGSLDTAEDYYATYSPLWGQYPEALLFVNWLYNHSSVLHERSE
                     IIKCAEMLIQEDDFEICESILRQQEHLWKRIDKTLSEEIVQKCRNMNGEYIWPWILPF
                     SAAGMKHSSIQYQ"
     gene            complement(1734..2918)
                     /locus_tag="OO544_003833"
     CDS             complement(1734..2918)
                     /locus_tag="OO544_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6769324.1"
                     /translation="MALTDIKVKTAKPKDKPYKLADGGGMYLLINTNGSKYWRMKYRF
                     AGKEKMLSIGVYPDVTLADAREKRSEARKLLAAGGDPGEAKKEEKIAQQMSLKNTFEA
                     IAREWHQSKADRWSLRYRDEIIDTFEKDIFPYIGKRPIAEIKPMELLEALRKMEKRGA
                     LEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLASALATPKKVHFPFLTANELPHFLND
                     LAGYTGSIITKTATQIIMLTGVRTQELRFARWEDIDFETKLWEIPAEVMKMKRPHIVP
                     LSEQVIMLFKQLEPISKHHPLVFIGRNDPRKPISKESINQVIELLGYKGRLTGHGFRH
                     TMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHALYLDGRREMMQWYADYIDSLSSR
                     ES"
     gene            complement(3218..3312)
                     /locus_tag="OO544_003834"
     tRNA            complement(3218..3312)
                     /locus_tag="OO544_003834"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3276..3278),aa:Sec,seq:tca)
     gene            3605..>4803
                     /locus_tag="OO544_003835"
     CDS             3605..>4803
                     /locus_tag="OO544_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HCR6769325.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTVVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     EDLRDIWQNDQWRIVGLLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSA"
BASE COUNT         1253 a         1055 c         1092 g         1403 t
ORIGIN      
        1 gggataccag ttcaaccgaa aacgccagag gaggggatta cccgctggca gggtaaatct
       61 gtggtttttg atgccagcta taacgtgttt gacgataaag tattacgaaa gaactttaat
      121 gagttcatgg agtaattcgt taaagtaatt tacaacgcta tccttttgca aaggaaactt
      181 tcagaaaata accccagggt aatcacctgg ggaaggaaca cagtgttatt attggataat
      241 aaattattac ttgttttaaa gttacactaa tgcaatatct actgatactg gatgcttgaa
      301 tgtttcatcc ctgctgcgga aaagggcaat atccagggcc agatatattc accgttcata
      361 tttctgcatt tctgtacaat ttcctcagaa agtgtcttgt caattctttt ccacaggtgc
      421 tcctgctgtc ttaaaatact ttcgcatatt tcgaaatcat cttcctgaat cagcatttcg
      481 gcgcatttaa tgatttctga tcgttcatgc agcacggagg aatggttata gagccagttt
      541 acaaacagca atgcttccgg atattgtccc cataatggtg agtatgttgc atagtaatct
      601 tctgccgtat ccagagagcc ggagaagtca tgccacacag caagtggaaa tggatgcaga
      661 tttccgcaca gcatttctgc gatgtaagtg tttgtggcaa agccatggcg aaaagctgtg
      721 gcggccttaa catgttcccc gttcagaaaa tgcaggagac cgagttcata ccagtaaggt
      781 gaaaattcat cagcatgctc tttcagtaca ctgaaggctc gctcatgatc gccggtgcgt
      841 aacagctctg aacccaacaa ccagcgagcc ccctgattat cttcaggatt gtatgccagt
      901 attttatcag tgagcataac cgcatcctga tggcgctgta agtgcacatt tgcgagaatt
      961 gctgcataaa gcgcgcgcag ataaggccgg ttttcgggat gcatccagat tatttcacca
     1021 cagaaacttt ccgggatgat acgattacct gccgccagtc ctttaagaga ggcgttcagt
     1081 gccttacgcg gggcattctg ttccagaaat gcatatgcca gatgagcgtg tatatcaata
     1141 aagtccggct cccgttgagc aaggcgtcgg agtttgttga tgtaactcgt ctcgctgatt
     1201 tcatcagcgt cgtatgcatc aagcacttct tcaaactccc ccagcaatgg gccaaaccag
     1261 gtatccggaa atacgaaata acccttgtta tcgtgcactt caaagcataa tctatcgttc
     1321 atttctgagc ctgtaaaaca tgtggtgata tagtctgaga gcataaaagt tgagattctg
     1381 tgtatcaagt cctgtcttgg caccgaatga atattgctta cgaagcccct tcatgctttc
     1441 cttgagaaca ataattttat ctttatgtga atctcatttt ttataatgat gtcagatgat
     1501 tgaaatgcta aattatatct gaatcaactt ttcctttgct aaaggtatcg aaataactgc
     1561 aaggctgtat tttgtaattg ccaatttaaa aatgtcaatt tgtttttaat tatgtttttg
     1621 taataagttt gaagacgcaa aaaaaataat ataataacaa aaatcctcca gccttgtagg
     1681 ccagaggata aaatacttaa ttgaggaaaa tatcagggaa aagtatttct gacttaactt
     1741 tcccggctgg agagtgaatc aatgtaatca gcgtaccatt gcatcatttc acggcggcca
     1801 tcgagataca gggcatgatt ataggtaccc ctgatggaat ttttatccac atgagctaac
     1861 tgcatttcaa tccaggcgga attaaagcct tgttcatgca gaattgtact catggtgtgt
     1921 ctgaaaccgt gccctgtgag tcttccctta tatccaagta attcaattac ttggttaatg
     1981 ctttccttac taattggttt gcggggatca tttctaccga taaagactag gggatgatgt
     2041 tttgagattg gttcaagctg cttaaatagc ataatgacct gctcagagag cggcacaata
     2101 tgaggtcgct tcatcttcat tacttctgcc gggatctccc acaattttgt ctcaaaatcg
     2161 atatcttccc agcgtgcaaa acgcagttct tgtgttcgta cgccggttaa cataatgatc
     2221 tgagtagctg tcttagtgat tatacttccg gtataacccg ccagatcgtt gaggaagtgg
     2281 ggaagttcat tagcagtaag aaagggaaaa tgtacttttt tcggtgtagc taaagcactg
     2341 gcaagatcgg gggctgggtt atagtctgcc cggccagtaa caattgcata gcggaacacc
     2401 tcaccacagc gctggcggac cttgcgcatt ttctccagag ctccacgttt ttccatttta
     2461 cgcagtgctt ccagtaactc cattggtttg atttcggcaa tagggcgctt gccaatgtag
     2521 ggaaaaatgt ctttttcaaa ggtatcaatg atttcatcac gatagcgtaa tgaccaccga
     2581 tcagcttttg actggtgcca ttcgcgagca atagcctcga acgtattttt cagactcatt
     2641 tgctgcgcga ttttttcttc cttttttgcc tcccctggat cgcctccagc agcgaggagt
     2701 ttcctggctt cactgcgttt ctcgcgagca tcggctagcg ttacatcggg gtatacgcca
     2761 atagaaagca tcttttcttt accagcaaag cggtacttca tgcgccagta tttagaacca
     2821 ttagtgttaa tcagtagata cataccgccg ccatcagcaa gcttataggg tttgtctttg
     2881 ggttttgcgg ttttcacttt gatatcggtt aatgccatga catcctccgg cttttgatga
     2941 attcatgttc ctgaggggga tttctgccag taactaacct gataccccca atacacccct
     3001 tgtataacaa agatattgtt aaaagccgtt gttatgtatc acatttttcc tattatgggg
     3061 gtgcttgtat ttcaatagga aataacaccc cctaaagtac ccccaaaaga aacttgatat
     3121 ggggtgaagt aggttgacct caggatacaa ataaatactg atatacaagg gattagaaga
     3181 gatttattga cattgggaga gttcagaaga ggtaaactgg cggaagatca caggagtcga
     3241 acctgcccgg gaccgctggc ggccccaact ggatttgaag tccagccgcc tcaccggaga
     3301 cgacgatctt ccgcgcctcg attgctacat ggaggcgggg cgcattatag ctacttcctt
     3361 gagtttctac atcccccaga tcagtttttt ccgcctaata cacgcagcgt attatcacga
     3421 aagcattata actcatataa aacccatatt ttacaatgat atatgagcgg aattaacgct
     3481 accacgatca cataaaacaa gaatcataaa ttaataacca gatatcggaa tattcgctct
     3541 cacagggatg gtttacaaaa tgcgttatcg gtctcagcac ccctatagca tcaaggaaaa
     3601 gcagatgaag agtgaagtgt tgtccgttaa agagaaaatt ggttatggca tgggagacgc
     3661 cgccagccac attattttcg ataacgtaat gttatatatg atgttctttt ataccgatat
     3721 ttttggcatt cctgccggat ttgtcggaac catgtttttg gtcgctcgtg cactggatgc
     3781 gatttccgat ccttgcatgg ggttgttggc cgatcgaacg cgctctcgct ggggtaaatt
     3841 tcgtccgtgg gtactgtttg gcgcactgcc attcgggatc gtctgtgtac tggcctatag
     3901 cacgccagat ctcagtatga acggcaaaat gatctatgca gcaattactt acaccctact
     3961 taccttactt tataccgtcg tcaatatccc ttactgcgca ttgggtggtg taatcaccaa
     4021 tgacccgact cagcgtatct cgctgcaatc ctggcgtttt gtgctggcga ccgcgggagg
     4081 catgctttct actgttctga tgatgccact ggttaattta attggcggtg ataataaacc
     4141 actcggtttc cagggcggta tcgcggtcct ttccgtggtg gcattcatga tgctggcatt
     4201 ttgtttcttc accactaaag aacgcgttga agcaccacct acaacaacgt ctatgcggga
     4261 agatttacgt gatatctggc aaaacgacca gtggcggatt gtcggtttac taaccatttt
     4321 caatatcctg gcggtgtgcg tacgcggtgg ggcgatgatg tattacgtca catggatttt
     4381 gggcacgccg gaagtgtttg tcgcttttct caccacttat tgcgtgggta acctgattgg
     4441 ttccgcactg gcaaaacctc tgaccgactg gaaatgtaaa gtcactatct tctggtggac
     4501 gaacgccctg ctggcagtga ttagcctcgc gatgttcttt gttcccatgc aggccagcat
     4561 cactatgttt gtcttcatct tcgtgattgg tgtgttgcat caactggtga cacctatcca
     4621 gtgggtaatg atgtccgata ccgtcgacta cggcgagtgg tgcaatggta aacgcctgac
     4681 cgggatcagt tttgctggca cgctgtttgt gctcaaactg gggttggcct tcggcggcgc
     4741 tcttatcggc tggatgctgg cttatggcgg atatgatgcg gcagaaaaag cgcagaacag
     4801 cgc
//
LOCUS       DAJTZL010000231         4798 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.232, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000231 DAJTZL010000000
VERSION     DAJTZL010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4798
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.232"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            241..582
                     /gene="raiA"
                     /locus_tag="OO544_003836"
     CDS             241..582
                     /gene="raiA"
                     /locus_tag="OO544_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HCR6769326.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    685..810
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            686..733
                     /gene="pheL"
                     /locus_tag="OO544_003837"
     CDS             686..733
                     /gene="pheL"
                     /locus_tag="OO544_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HCR6769327.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            833..1993
                     /gene="pheA"
                     /locus_tag="OO544_003838"
     CDS             833..1993
                     /gene="pheA"
                     /locus_tag="OO544_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HCR6769328.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQQQN
                     FTRFVVLARKAINMSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEIFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(2036..3157)
                     /gene="tyrA"
                     /locus_tag="OO544_003839"
     CDS             complement(2036..3157)
                     /gene="tyrA"
                     /locus_tag="OO544_003839"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HCR6769329.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(3168..4238)
                     /gene="aroF"
                     /locus_tag="OO544_003840"
     CDS             complement(3168..4238)
                     /gene="aroF"
                     /locus_tag="OO544_003840"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HCR6769330.1"
                     /translation="MQKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            4447..>4798
                     /locus_tag="OO544_003841"
     CDS             4447..>4798
                     /locus_tag="OO544_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HCR6769331.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVESASQ
                     LHEVWQKGADENAST"
BASE COUNT         1311 a         1229 c         1096 g         1162 t
ORIGIN      
        1 tgagctgccg tttttttatt ctgttagttg tgaaactgaa gcgatttagt cactatcgat
       61 ctcatcaaat atggctcgct ttgagatatt cctcaagtaa aaaaacacct cttcctgcga
      121 tttctcacaa aaaagattcg ttgacaaaaa gtgacaaaat tatgagattc ccatcacaca
      181 ttttgacatc aggaacggta tgctgaattc accaagacgg gaagacaaga ggtaaaattt
      241 atgacaatga acattaccag caaacaaatg gaaattactc cggccatccg ccaacatgtc
      301 gcagaccgtc tcgccaaact ggaaaaatgg caaacacatc tgattaatcc acatatcatt
      361 ctgtccaaag agccacaagg gtttgttgct gacgccacaa tcaatacacc taacggcgtt
      421 ctggttgcca gtggtaaaca tgaagatatg tacactgcaa ttaacgaatt gatcaacaag
      481 ctggaacggc agctcaataa actgcagcac aaaggcgaag cacgtcgtgc cgcaacatcg
      541 gtgaaagacg ccaacttcgt cgaagaagtt gaagaagagt agtcctttat attgagtgga
      601 tcgccaacgc gccttcgggc gcgttttttg ttgacagcgt gaaaacagta cgggtactgt
      661 actaaagtca cttaaggaaa caaacatgaa acacacaccg tttttcttcg cattcttttt
      721 taccttcccc tgaatgggag gcgtttcgtc gtgtgaaaca gaatgcgaag acgaacaata
      781 aggcctccca aaccgggggg ccttttttat tgataacaaa aaaggcaaca ctatgacatc
      841 ggaaaatcca ttactggcgc tgcgagagaa aatcagcgcg ctggatgaaa aattattagc
      901 attactggca gagcggcgcg aactggccgt cgaggtggga aaagccaaac tgctctcgca
      961 tcgcccggta cgagatattg atcgtgaacg cgatttactg gaaagattaa ttacgctcgg
     1021 taaagcgcac catctggacg cccattacat tactcgcctg ttccagctca tcattgaaga
     1081 ttccgtatta actcagcagg ctttgctcca gcaacatctc aataaaatta atccgcactc
     1141 agcacgcatc gcttttctcg gccccaaagg ttcttattcc catcttgcgg cgcgccagta
     1201 tgctgcccgt cactttgagc aattcattga aagtggctgc gccaaatttg ccgatatttt
     1261 taatcaggtg gaaaccggcc aggccgacta tgccgtcgta ccgattgaaa ataccagctc
     1321 cggtgccata aacgacgttt acgatctgct gcaacatacc agcttgtcga ttgttggcga
     1381 gatgacgtta actatcgacc attgtttgtt ggtctccggc acgactgatt tatccaccat
     1441 taatacggtc tacagccatc cgcagccatt ccagcaatgc agcaaattcc ttaatcgtta
     1501 tcctcactgg aagattgaat ataccgaaag tacgtctgcg gcaatggaaa aggttgcaca
     1561 ggcaaaatca ccgcatgttg ctgcgttggg aagcgaagct ggcggcactt tgtacggttt
     1621 gcaggtactg gagcgtattg aagcgaatca gcaacaaaac ttcacccgat ttgtggtgtt
     1681 ggcacgtaaa gccattaaca tgtctgacca ggttccggcg aaaacgacgt tgttaatggc
     1741 gaccgggcaa caagccggtg cgctggttga agcgttgctg gtgctgcgca accacaatct
     1801 gattatgacc cgtctggaat cacgcccgat tcacggtaat ccatgggaag agatatttta
     1861 tctggatatt caggccaatc ttgaatcagc ggaaatgcaa aaagcattga aagagttagg
     1921 ggaaattacc cgttcaatga aggtattggg ctgttaccca agtgagaacg tagtgcctgt
     1981 tgatccaacc tgatgaaaag gtgccggatg atgtgaatca tccggcactg gattattact
     2041 ggcggttgtc attcgcctga cgcaataaca cgcggctttc actctgaaaa cgctgtgcgt
     2101 aatcgccgaa ccagtgctcc accttgcgga aactgtcaat aaacgcctgc ttatcgccct
     2161 gctccagcaa ctcaatcgcc tcgccgaaac gcttatagta acgtttgatt aacgccagat
     2221 tacgctctga cgacataata atgtcggcat aaagctgcgg atcctgagca aacagtcgcc
     2281 cgaccatcgc cagctcaagg cggtaaatcg gcgaagagag cgccagaagt tgctcaagct
     2341 gaacattttc ttctgccaga tgcagcccat aagcaaaagt agcaaagtgg cgcagagcct
     2401 gaataaacgc catattctga tcgtgctcga cagcgctaat acgatgcagc cgagcgcccc
     2461 agacctgaat ttgctccaga aaccattggt atgcttccgg cttacgtcca tcacaccaga
     2521 ccacaacttg ctttgccagg ctaccgctgt ccgggccgaa catcgggtgt aaccccagta
     2581 ccgggccatc gtgcgccgcc agcatggcct gtaatggtcc atttttcact gatgccagat
     2641 caaccagaat acaatctttc ggtaaaggcg gtaatttgcc gataacttgc tcagtaacgt
     2701 ggattggcac actaacaatc accattccgg catcggcaac aatatcagcc gctcgatccc
     2761 agtcatgttg ctccagaatc cgcacctgat aacccgagag tgtcagcatc ttctcgaaca
     2821 ggcgtcccat ctgaccgcca ccgccgacga taaccaccgg acgcagtgac ggacaaagtg
     2881 ttttaaatcc tttgtcgttt tcactggagt aagattcacg catcacccga cgcaaaacat
     2941 cctcaatcag atctggcggt acacccagag cttccgcctc tgcacgacgc gaggccaaca
     3001 tagatgcctc gcgctccgga acataaatag gcagtccaaa gcggcttttc acctcgccca
     3061 cttcagcaac cagttccaga cgcttcgcta ataaattcag cagcgcttta tcgacttcat
     3121 caatttgatc gcgtaatgcg gtcaattcag caaccataat aaacctctta agccacgcga
     3181 gccgtcagct gcccgttcag atcctgatga atttcacgca gcaaggcatc ggtcatttcc
     3241 cagctaatgc aggcatcggt tacggataca ccgtatttca tttcactgcg cggttgctca
     3301 gaagactgat tgccctcgtg gatattactt tcgatcatca gaccaataat tgagcgattg
     3361 ccatctttga tttgagcaac cacggattct gccaccgcag gctgacggcg ataatcttta
     3421 ttggaattac cgtggctgca atctaccatc agagacgggc gcagtcccgc ctgttccatc
     3481 tctttttcac attgcgcaac atccgcaggg ctatagttcg gcgctttacc accgcgcagg
     3541 atgacatgcc cgtccggatt cccctgagtt tgtagcaacg caacctgccc tgcctggtta
     3601 atgccaacaa aacggtgcgg ctgggcggcg gcgcgcatag cgttaattgc tgttgccaga
     3661 ctgccgtcgg tgccgttttt aaaaccaacc ggcatggaaa gcccggaggc catttcacgg
     3721 tgagtttgcg attccgttgt acgagcacca attgctgacc agctaaacag atcgcccagg
     3781 tattgcgggc tattcggatc taacgcttcc gtcgccagtg gcagtcccat attcaccagc
     3841 tcaagcagca atttacgcgc gatctgcagc ccggcttcta catcaaaaga gccatccata
     3901 tggggatcgt taattaaccc tttccagccg acagtggtac ggggtttttc aaaatagacg
     3961 cgcattacca gatagaggct atcgctgacc tctgcggcaa gggctttaaa tcgacgagca
     4021 tattccagag cagtttccgg atcatgaatg gaacaaggac cacaaactac cagcagacga
     4081 ggatcgcgcc cggcgataat atctgaaatg gttttacgcg agtcagcaat ctgggcttct
     4141 tgttgcaggc tcaatggaaa agcggccttc agttgttccg gagtcattaa aacctgttcg
     4201 tcggtaatat gtacgttatt cagcgcgtct ttttgcatga tggcgatcct gtttatgctc
     4261 gtttgcgata gttgatcctc agcgaggatg acgtaacgat aacacataaa gtaaagtttt
     4321 caatccatat ttcgtacatt tttctttaca cgaacaattt gctcgcgctt ttgatgctta
     4381 tttcgtacaa ataaatttac acctccttta aaaacaatcc gctatgcttt gaaaaaggag
     4441 aaagaaatga tgaaaaagtt tatcgccccc ttattggctt tactggttag cggatgtcag
     4501 attgatcctt atactcacgc gccaaccttg accagcaccg actggtatga tgtcggtatg
     4561 gaagatgcga tatcgggcag cgccataaaa gatgacgatg catttagcga ttcacaggcg
     4621 gatcgcggtc tataccttaa aggatatgcc gaaggacaaa agaaaacttg ccagaccgat
     4681 tttacttatg cccggggact ttccggtaaa agctttcctg ccagctgtaa caatgttgaa
     4741 agtgccagcc aactgcatga agtatggcaa aaaggggctg atgaaaatgc cagcacaa
//
LOCUS       DAJTZL010000232         4775 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.233, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000232 DAJTZL010000000
VERSION     DAJTZL010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4775
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.233"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(51..494)
                     /locus_tag="OO544_003842"
     CDS             complement(51..494)
                     /locus_tag="OO544_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCR6769332.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEVLVR
                     SFLPEAPLWVAVNERDQPVGFMLLSGQHMDALFIDPDVCGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVEE"
     gene            651..1580
                     /gene="metA"
                     /locus_tag="OO544_003843"
     CDS             651..1580
                     /gene="metA"
                     /locus_tag="OO544_003843"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HCR6769333.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLDPDVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            1849..3450
                     /gene="aceB"
                     /locus_tag="OO544_003844"
                     /pseudo
     CDS             1849..3450
                     /gene="aceB"
                     /locus_tag="OO544_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
     gene            3480..4771
                     /gene="aceA"
                     /locus_tag="OO544_003845"
                     /pseudo
     CDS             3480..4771
                     /gene="aceA"
                     /locus_tag="OO544_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
BASE COUNT         1189 a         1257 c         1208 g         1121 t
ORIGIN      
        1 tgcgacgctg gcgcgtcttc tccagcctgg gcaggcacgg caggggcagc ttattcctcc
       61 acatacgcca gattcagcaa cggatacggt ttccccaaat cgtccacctc agagcgtccc
      121 gtaaccttaa aacccacctt cttatagaac ccaaccgcct gctcattttg ctcattaacg
      181 ttggttgtca gttccggtgc catcgagagc gcatgctcca ccagcatccg acccacgccg
      241 cagccgcaca catcaggatc gataaacagc gcatccatat gctgcccact taacaacata
      301 aacccaaccg gctgatcccg ttcattaacc gcgacccaca acggcgcttc cggcaggaag
      361 gaacgaacta gcacctccag ctcggcccga tactctgctg ataaaaaatc gtgtgtggca
      421 tcgacagaac gacaccaaat cgcaacgagt tcctcccctt cctcatgccg tgagcggcga
      481 atactaataa ccattttctc tccttttcgt cattcttata ttctaacgta gccttttcct
      541 tgaaactttc tcaccttcaa catgcaggct cgacattggc aaattttctg gttatcttca
      601 gctatctgga tgtctaaacg tataagcgta tgtagtgagg taatcaggtt atgccgattc
      661 gtgtgccgga cgagctaccc gccgtcaatt tcttgcgtga agaaaacgtc tttgtgatga
      721 caacttctcg tgcgtctggt caggaaattc gtccacttaa ggttctgatc cttaacctga
      781 tgccgaagaa gattgaaact gaaaatcagt ttctgcgcct gctttcaaac tcacctttgc
      841 aggtcgatat tcagctgttg cgcatcgatt cccgtgaatc gcgcaacacg cccgcagagc
      901 atctgaacaa cttctactgt aactttgaag atattcagga acaaaacttt gacggtctga
      961 tagttaccgg cgcgccgctg ggcctggtgg agtttaatga tgtcgcttac tggccgcaga
     1021 tcaaacaggt gctggagtgg tcgaaagatc acgtcacctc gacgctgttt gtctgctggg
     1081 cggtacaggc cgcgctcaat atcctctacg gcattcctaa gcaaactcgc accgacaaac
     1141 tctctggcgt ttacgagcat catattctcc atcctcatgc gcttctgacg cgtggctttg
     1201 atgattcatt cctggcaccg cattcgcgct atgctgactt tccggcagcg ttgattcgtg
     1261 attacaccga tctggaaatt ctggcagaga cggaagaagg ggatgcatat ctgtttgcca
     1321 gtaaagataa gcgcattgcc tttgtgacgg gccatcccga atatgatgcg caaacgctgg
     1381 cgcaggaata tttccgcgat gtggaagccg gactagaccc ggatgtaccg tataactatt
     1441 tcccgcacaa tgatccgcaa aacaaaccgc gcgcgagctg gcgtagtcac ggtaatttgc
     1501 tgtttaccaa ctggctcaac tattacgtct accagatcac gccatacgat ctacggcaca
     1561 tgaatccaac gctggattaa tcttctgtga tagtcgatcg ttaagcgatt cagcacctta
     1621 cctcaggcac cttcgggtgc cttttttatt tccgaaacgc acctcagcaa gtgaataaat
     1681 tttattcata ttgttatcaa taagttatca agtattttta attaaaatgg aaattgtttt
     1741 tgattttgca ttttaaatga gtagtcttag ttgtgctgaa cgaaaagcgc acaacgatcc
     1801 ttcgttcaca gtggggaagt tttcggatcc atgacgagga gctgcacgat gactgaacag
     1861 gcaacaacaa ccgatgaact ggctttcaca aggccgtatg gcgagcagga gaagcaaatt
     1921 cttactgccg aagcggtaga atttctgact gagctggtga cgcattttac gccacaacgc
     1981 aataaacttc tggcagcgcg cattcagcag cagcaagata ttgataacgg aacgttgcct
     2041 gattttattt cggaaacagc ttccattcgc gatgctgatt ggaaaattcg cgggattcct
     2101 gcggacttag aagaccgccg cgtagagata actggcccgg tagagcgcaa gatggtgatc
     2161 aacgcgctca acgccaatgt gaaagtcttt atggccgatt tcgaagattc actggccccg
     2221 gactggaaca aagtgatcga cgggcaaatt aacctgcgtg atgcagttaa cggcaccatc
     2281 agctatacca atgaagcagg caaaatttat cagctcaagc ccaatccagc ggttttgatt
     2341 tgtcgggtac gcggtctaca cttgccggaa aaacatgtca cctggcgtgg tgaggcaatc
     2401 cccggtagcc tgtttgattt tgcgctctat ttcttccaca actatcaggc actgttggca
     2461 aagggcagcg gtccctattt ctatctgccg aaaacccagt cctggcagga agcggcctgg
     2521 tggagcgaag tcttcagcta tgcagaagat cgctttaatc tgccgcgcgg cgccatcaag
     2581 gcgacgttgc tgattgaaac gctgcccgcc gtgttccaga tggacgaaat ccttcacgcg
     2641 ctgcgcgacc atattgttgg tctgaactgc ggtcgctggg attacatctt cagctatatc
     2701 aaaacgttga aaaactatcc cgatcgtgtc ctgccagaca gacaggccgt gacgatggat
     2761 aaaccattcc tgaatgctta ctcacgcctg ctgattaaaa cctgccataa acgcggtgct
     2821 tttgcgatgg gcggcatggc agcgtttatt ccgagcaaag atgaagagcg taataaccag
     2881 gtgctcaaca aagtaaaagc ggataaagcg ctggaagtca ataacggtca cgatggcaca
     2941 tggatcgctc acccaggcct tgcggacacg gcaatggcgg tattcaacga cattctcggc
     3001 tcccgtaaaa atcagcttga agtattgcgc gaacaagacg cgccgattac tgccgatcag
     3061 ctgctggcac cttgtgacgg tgaacgcacc gaagaaggta tgcgcgccaa cattcgcgtg
     3121 gctgtgcagt acatcgaagc gtggatctcc ggcaacggct gcgtgccgat ttatggcctg
     3181 atggaagatg cggcgacggc tgaaatttcc cgtacctcga tctggcagtg gatccatcat
     3241 caaaaaacgt tgagcaatgg caaaccggtg accaaagcct tgttccgcta gatgctgggc
     3301 gaagagatga aagtcattgc cagcgaactg ggcgaagaac gtttctccca gggccgtttt
     3361 gacgatgccg cacgcttgat ggaacagatc accacttccg atgagttaat tgatttcctg
     3421 accctgccag gctaccgcct gttagcgtaa accaccacat aactatggag catctgcaca
     3481 tgaaaacccg tacacaacaa attgaagaat tacagaaaga gtggactcaa ccgcgttggg
     3541 aaggcattac tcgcccatac agtgcggaag atgtggtgaa attacgcggt tcagtcaatc
     3601 ctgaatgcac gctggcgcaa ctgggcgcag cgaaaatgtg gcgtctgctg cacggtgagt
     3661 cgaaaaaagg ctacatcaac agcctcggtg cactgactgg cggtcaggcg ctgcaacagg
     3721 cgaaagcggg tattgaagca gtctatctgt cgggatggca ggtagcggcg gacgctaacc
     3781 tggcggccag catgtatccg gatcagtcgc tctatccggc aaactcggtg ccagctgtgg
     3841 tggagcggat caacaacacc ttccgtcgtg ccgatcagat ccaatggtcc gcgggcattg
     3901 agccgggcga tccgcgctat gtcgattact tcctgccgat cgttgccgat gcggaagccg
     3961 gttttggcgg tgtcctgaat gcctttgaac tgatgaaagc gatgattgaa gccggtgcag
     4021 cggcagttca cttcgaagat cagctggcgt cagtgaagaa atgcggtcac atgggcggca
     4081 aagttttggt gccaactcaa gaagctattc agaaactggt cgcggcgcgt ctggcagctg
     4141 acgtgacggg cgttccaacc ctactggttg ctcgtaccga tgccgatgca gcggatctga
     4201 tcacctccga ttgcgacccg tatgacagcg aatttattac cggcgagcgt accagtgaag
     4261 gcttcttccg tactcatgcg ggcattgagc aagcgatcag ccgtggcctg gcgtatgcgc
     4321 catatgctga cctggtctgg tgtgaaacct ccacgccgga tctggaactg gcgcgtcgct
     4381 ttgcacaagc tatccacgcg aaatatccgg gcaaactgct ggcttataac tgctcgccgt
     4441 cgttcaactg gcagaaaaac ctcgacgaca aaactattgc cagcttccag cagcagctgt
     4501 cggatatggg ctacaagttc cagttcatca ccctggcagg tatccacagc atgtggttca
     4561 acatgtttga cctggcaaac gcctatgcct agggcgaggg tatgaagcac tacgttgaga
     4621 aagtgcagca gccggaattt gccgctatac cttcgtatct caccagcagg aagtgggtac
     4681 aggttacttc gataaagtga cgactattat tcagggcggc acgtcttcag tcaccgcgct
     4741 gaccggctcc actgaagaat cgcagttcta agcaa
//
LOCUS       DAJTZL010000233         4708 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.234, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000233 DAJTZL010000000
VERSION     DAJTZL010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4708
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.234"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            387..1469
                     /locus_tag="OO544_003846"
     CDS             387..1469
                     /locus_tag="OO544_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpD"
                     /protein_id="HCR6769334.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYARLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNNQ
                     VIYGNNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEV
                     VAQYQFDFGLRPSVAYLQSKGKDLGAWGDQDLVEYIDVGATYYFNKNMSTFVDYKINL
                     IDKSDFTKASGVATDDIVAVGMVYQF"
     gene            complement(1658..2041)
                     /gene="quuD"
                     /locus_tag="OO544_003847"
     CDS             complement(1658..2041)
                     /gene="quuD"
                     /locus_tag="OO544_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein QuuD"
                     /protein_id="HCR6769335.1"
                     /translation="MRDIQMVLERWGAWAANNHEDVTWSSIAAGFKGLITSKVKSRPQ
                     CCDDDAMIICGCMARLKKNNSDLHDLLVDYYVVGMTFMSLAGKHCCSDGYIGKRLQKA
                     EGIIEGMLMALDIRLEMDIVVNNSN"
     gene            complement(2127..2267)
                     /locus_tag="OO544_003848"
     CDS             complement(2127..2267)
                     /locus_tag="OO544_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HCR6769336.1"
                     /translation="MMFEFYMAELLRHRWGHLRLYRFPGSVLTDYRILKNYAKTLTGA
                     GV"
     gene            complement(2264..2626)
                     /gene="rusA"
                     /locus_tag="OO544_003849"
     CDS             complement(2264..2626)
                     /gene="rusA"
                     /locus_tag="OO544_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RusA"
                     /protein_id="HCR6769337.1"
                     /translation="MNTYSITLPWPPSNNRYYRHNRGRTHISAEGQAYRDNVTRIIKN
                     AMLDIGLAMPVKIRIECHMPDRRRRDLDNLQKAAFDALTKAGFWLDDAQVVDYRVVKM
                     PVTKGGRLELTITEMGNE"
     gene            complement(2623..2913)
                     /locus_tag="OO544_003850"
     CDS             complement(2623..2913)
                     /locus_tag="OO544_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HCR6769338.1"
                     /translation="MADLRKAARGRECQVRIPGVCNGNPETSVLAHIRLAGLCGTGIK
                     PPDLIATIACSACHDEIDRRTYFVDAEYAKECALEGMARTQVIWLKEGVIKA"
     gene            complement(2906..3076)
                     /locus_tag="OO544_003851"
     CDS             complement(2906..3076)
                     /locus_tag="OO544_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6769339.1"
                     /translation="MATPLIRVMNGHIYRVSNRRKRKPELKPSEIPTLLGYTASLVDK
                     KWLRLAARRNHG"
     gene            complement(3076..3531)
                     /locus_tag="OO544_003852"
     CDS             complement(3076..3531)
                     /locus_tag="OO544_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbcN family protein"
                     /protein_id="HCR6769340.1"
                     /translation="MTLPVDGIKLHRGNFAAIGQQIQPLLDAGQCLRLQVKPWREKRS
                     LSQNALSHMWYTEISEYLIARGKTFATPEWVKDAMKHTYLGYESKDRVDVVSGEVTTV
                     QSLRHTSELETGEMYIFLCKVEAWAMNIGCHLTIPQSCEYQQLRDKQEA"
     gene            3796..4173
                     /locus_tag="OO544_003853"
     CDS             3796..4173
                     /locus_tag="OO544_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6769341.1"
                     /translation="MTNKENKDKYINKFKKELSKYIQPTAGVDIQLFNSKDGGGVIKA
                     TLNRSGKRKSSIAGNFTKLGEAITSSGQRVFGGDLSNVNFYGTNTIFDGDTIFLIKSP
                     DSEEWSSQKASKDVEGIVFGGKK"
BASE COUNT         1352 a         1050 c          980 g         1326 t
ORIGIN      
        1 ttttttacgt acgtaaacca ctaaatgata tttgcgagaa tgctaccgag tattgaaaac
       61 accactacaa atacataagc aaatcccaac aaataaccaa caaataattt ccagtgttat
      121 ttttagccgg tttaaattga accttcaaat tatagagcac ttataaataa cagccgttaa
      181 tataaattgg ctaatagatt tatttttatt cagtcaagag ccatgaatag gattcgatag
      241 aaaaaagttc agataaaaat agagatctac ttcacaaatc aaacgagaaa ccaaaactta
      301 catcttgaaa taatcacatt gattagatga atatttatcg cgcaatgaca tcatttttta
      361 ataatagttc aaaaaaaggg ctcacgatga aaaaattaac agtggcaatt tctgctgtag
      421 ctgcatcagt actgatggcg atgtctgctc aggcagctga aatttataat aaagacagta
      481 acaagctgga tctgtacggg aaagttaatg ctaagcacta cttctcctct aatgatgcag
      541 atgatggtga tactacttat gcccgtcttg gcttcaaagg tgaaacccaa atcaacgatc
      601 aactgactgg tttcggtcag tgggaatatg aattcaaagg caaccgcgct gaatctcaag
      661 gttcctccaa agataaaacc cgtcttgcct tcgctggcct gaaattcggt gactacggct
      721 ccatcgatta cggccgtaac tacggtgtag catacgacat cggtgcgtgg actgacgtcc
      781 tgccagaatt cggtggtgac acttggactc aaacagatgt gttcatgact ggtcgcacca
      841 ctggtgttgc aacttatcgt aacaacgact tctttggtct ggttgatggt ctgaactttg
      901 ctgctcagta tcagggtaaa aatgaccgca ctgacgtaac tgaagctaat ggtgatggtt
      961 tcggtttctc cactacttat gagtatgaag gattcggtgt aggtgcaacc tatgctaaat
     1021 ctgaccgcac taataatcag gttatctacg gtaacaacag cctgaatgca tctggtcaaa
     1081 atgctgaagt atgggcagct ggtctgaaat atgatgcgaa caacatctat ctggctacca
     1141 cctattctga aacccagaac atgactgttt ttggtaataa ccatattgcc aacaaagcac
     1201 aaaacttcga agtagttgca caatatcagt tcgacttcgg tctgcgtccg tccgttgctt
     1261 acctgcaatc taaaggaaaa gacttgggtg cgtggggtga tcaggacctg gttgaatata
     1321 ttgatgtagg tgcaacctat tacttcaaca aaaatatgtc cacttttgtt gattacaaaa
     1381 tcaacctgat tgataagagc gatttcacga aagcatctgg cgttgctacc gatgatatcg
     1441 ttgctgtagg tatggtttac cagttctaat ttgattacta aaagatatgt tgcgggaggc
     1501 tttgcctccc caacatataa gtggctccct caagccactt cctttaggag cacaaccttg
     1561 cttctaacta tataaacctt ctgttatata ttacccttta tttttggggg cgttgcaacg
     1621 ccccattttt aataattttt agtaaacaat tggcatatta attagagtta ttaacaacga
     1681 tatccatctc taaccggata tctaatgcca ttaacatccc ttcaattatg ccctcagcct
     1741 tctgtaacct tttcccgata taaccatcag agcagcaatg cttacctgcc agtgacatga
     1801 atgtcatacc gactacataa taatctacta ataaatcgtg caaatcgctg ttgttctttt
     1861 tcagacgggc catgcacccg caaataatca tcgcgtcatc gtcacaacat tgcgggcgag
     1921 attttacttt tgaagtaatt aatcccttaa aaccggcggc aatggacgac caggtcacat
     1981 cttcatgatt attagccgcc cacgctcccc aacgctcaag aaccatctga atatcacgca
     2041 tcaacttact ccacaaaaat cagaccagaa cgccaattac aagcaaaaat caacaaaaca
     2101 gtattagttg attgttatct ctgacttcat actcctgctc ctgtcagggt tttggcgtaa
     2161 ttcttcagta ttcggtaatc ggtcaaaaca gaaccgggga aacgatataa gcgcagatgc
     2221 ccccagcggt ggcgaagaag ttctgccata taaaactcaa acatcattca ttccccattt
     2281 cggtgatggt cagttccagc ctcccacctt tggtaacagg catcttcaca acgcggtaat
     2341 caacgacctg agcatcatcc agccagaaac ctgctttggt gagtgcgtca aaagcggctt
     2401 tttgcagatt atccaggtca cggcgacggc gatccggcat gtggcactca atacggattt
     2461 tcactggcat agccaggccg atatccagca ttgcgttttt aatgattcgg gtgacgttat
     2521 cgcggtatgc ctgcccttct gcgctgatgt gtgtgcgccc gcgattatgg cggtaatagc
     2581 ggttattgct cggaggccag ggtaatgtga tgctgtaggt attcacgcct taataacccc
     2641 ctctttcagc cagataacct gcgttctcgc cataccttcc agcgcgcatt cttttgcata
     2701 ctcagcatcg acaaaatatg tgcggcggtc gatttcgtcg tgacaggcag aacatgcaat
     2761 ggtggcaatc aggtctggcg gtttgatacc ggtaccgcac aatccagcca gccggatatg
     2821 tgccagtact gacgtttcag gattgccatt acatacgcca gggattctta cctggcattc
     2881 ccgaccacgc gctgcttttc tcaaatcagc catgattcct ccttgctgcc agtcgcaacc
     2941 attttttatc aaccaggcta gcggtatatc cgagcagtgt tggtatttcg gatggcttca
     3001 gctcaggctt acgcttacga cgatttgata ctctgtagat gtgcccgttc atgacacgaa
     3061 taagcggtgt agccattacg cctcctgctt atcgcgcagc tgctggtatt cacagctctg
     3121 cggaattgtc aggtggcagc cgatattcat cgcccaggct tcgactttgc acaggaagat
     3181 gtacatctcg ccggtttcca gctctgacgt atggcggagg gattggacgg tggtgacctc
     3241 gccggacacg acatctacac ggtctttgct ttcgtagccg agataggtgt gcttcatcgc
     3301 gtctttgacc cactcaggcg tagcgaaggt cttgccgcgg gcgatgagat attcgctgat
     3361 ctccgtgtac cacatgtggc tgagcgcgtt ctgcgacagg ctgcgcttct cgcgccacgg
     3421 cttaacctgc aggcgtaagc attgcccggc atccagcaat ggctgaatct gctggccgat
     3481 ggccgcgaag ttaccgcgat ggagtttgat gccgtctact ggcagagtca tacggcctcc
     3541 ctaacggaaa ccgcagaatg cagaaaatcg caggtgcatt tctgcatctg tgacaaggtg
     3601 atgagttcag attgtggtcg catttaagtc cccttaaatg cgcagaagtc accggagttg
     3661 ttcaggctcc gatgagagga ttatggcgga ttgattacga taaatcaaaa ctatcttgaa
     3721 gccaaaacag tgatcttcgc tttaaaaacc ttgtatactt tcacctaatc acagccagaa
     3781 ctatggatct aaccaatgac caacaaagaa aacaaagata aatatattaa caagttcaag
     3841 aaagagcttt caaaatatat acaaccaact gctggagtgg acattcaatt atttaactct
     3901 aaggatggtg gtggtgtaat taaagccact ttgaatagaa gtggaaaaag aaaatcatct
     3961 attgctggta attttactaa attaggtgag gcaattacct cgagtgggca gagggttttt
     4021 ggtggtgatc tatcaaatgt gaatttttac ggaacaaata cgatttttga tggagataca
     4081 atatttttaa ttaaatcacc tgattcagag gagtggtcat cacaaaaagc ttccaaagac
     4141 gtagagggca tcgtatttgg agggaaaaaa tgattaacat tactactaag gatgcactca
     4201 agtcagtggt gaagtaagag atctagtaat tcttcttgaa actgatggaa aaacggtgtc
     4261 tgattatttg cagccaatcc ctcccaaaaa agatacacac tggagcatga ttttcattcc
     4321 ggtagcaatt tatatactac ttaatcttgt atacattatt gctcacccat tatttcctga
     4381 aaaatatcta aaaacatcaa catctatatt tctattcatt tccttcatat taagtgcaat
     4441 agtagcatat tttgttttcc ataagcataa atctgccact ctatccatat gcagcttagt
     4501 gttcttagtg ttaattgtag gtgctaacgt agggtttatc agttacagcg acctaacaca
     4561 aaaagcgacg caaaaattag atagtttgtc taaataatta tgttgccatg cgaagtatgc
     4621 atggctgcat gtctgcatgt ctgccttcca tttaaaatgg gctaagacct ataaccctaa
     4681 atattattct ttattatctt ctttacca
//
LOCUS       DAJTZL010000234         4636 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.235, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000234 DAJTZL010000000
VERSION     DAJTZL010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4636
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.235"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..253)
                     /locus_tag="OO544_003854"
     CDS             complement(<1..253)
                     /locus_tag="OO544_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HCR6769342.1"
                     /translation="MKLNDSNLFRQQALINGEWLDANNGGIIDVTNPANGDKLGSVPK
                     MGADETRAAIDAANRALPAWRALTAKERANILRNWFNLMM"
     gene            complement(276..1544)
                     /gene="lhgO"
                     /locus_tag="OO544_003855"
     CDS             complement(276..1544)
                     /gene="lhgO"
                     /locus_tag="OO544_003855"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HCR6769343.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSQLEMERM
                     RALWERTAANGIEREWINAVELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSKHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILSSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            complement(1564..2541)
                     /gene="csiD"
                     /locus_tag="OO544_003856"
     CDS             complement(1564..2541)
                     /gene="csiD"
                     /locus_tag="OO544_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation induced protein CsiD"
                     /protein_id="HCR6769344.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            complement(2877..>4636)
                     /locus_tag="OO544_003857"
     CDS             complement(2877..>4636)
                     /locus_tag="OO544_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HCR6769345.1"
                     /translation="VYNINVTSPSGQQETFKILINPEHLRQTLERKSLTAVQKSQCEI
                     ITPKKPGEAILHAFNATYQQIRENMSEFARCHYGYIQIPPVTTFRADGPETPEEEKGY
                     WFHAYQPEDLCTIHNPMGDLQDFIALVKDAKKFGIDIIPDYTFNFMGIGGSGKNDLDY
                     PSADIRAKISKDIEGGIPGYWQGQVLIPFIKDPVTKERKQIHPEDIHLTAKDFEASKD
                     NISKDEWENLHALKEKRLNGIPKTTPKSDQVIMLQNQYVREMRKYGVRGLRYDAAKHS
                     KHEQIERSITPPLKNYNERLHNTNLFNPKYHKKAVMNYMEYLVTCQLDEQQMSSLLYE
                     RDDLSAIDFSLLMKTIKAFSFGGDLLTLASKPGSTISSIPSERRILININHDFPNNGN
                     LFNDFLFNHQQDEQLAMAYMAALPFSRPLVYWDGQVLKSTTEIKNYDGSTRVGGEAWL
                     NKGCSTYQQLYNEFHALYIDKAGIWSAFEGVFATKNVLAFSRGDSVNINHSPHDGLVI
                     INKGNEEVEGTWPNKLQPGIYKNMGSNSVNIIINNTRKIIPPGKVFTLRGGTLNINIP
                     GRSALLLGKTGEPPNYLYL"
BASE COUNT         1144 a         1090 c         1120 g         1282 t
ORIGIN      
        1 ccatcatcaa attgaaccag ttgcgcagaa tgttggcgcg ttctttggcg gtgagcgcac
       61 gccaggcggg cagggcgcgg ttggcggcgt cgatagcggc gcgggtttca tcagcgccca
      121 ttttcggcac gctacccagc ttgtcgccgt tcgccggatt ggtgacgtcg atgatcccgc
      181 cattgttggc gtccagccat tccccgttaa tcaacgcctg ctggcggaat aagttactgt
      241 cgttaagttt cattgttacg tcctgttttc aaaggttatt gattaaatgc ggcgtgtaag
      301 gcatccacac tacgtgccgc tcgcagcgtg cgtccggggt tactctggct tgccaacagc
      361 gtttgtacct tgctgacaat atgcgcacca ataggaattg ctgatgtcgc tgccggggag
      421 ggcgcattgc aggtgtggat cgtgcgtggg gtggtgacaa acagaaaatc gtcaatcagc
      481 ttgccgtccg gcgatactgc ctgcgcccgc acaccggcgg gccagggctg gagatcgctt
      541 aacgaaagcc ggggacaata cttttgcacc agccgcaggt agccgctttt gcacagcgag
      601 tttttcatct cgcccagtcc tgagcgtaga tggttttgca gcacccggcg aatccccgac
      661 gagctcaaaa tctccagcgt gtcactaaat gagaagtcgc gcttgcgata gccttcgcgt
      721 ttgaaagcca gcaccgcgtt tggcccgacg gtcacgctgc cgtcgatcat gcgggtgaga
      781 tgcacgccca aaaatggcat cgcggggtcg ggaatggggt aaatcaggtg gttaacaatc
      841 tggttatgct ccggcgcaag gcggaaatac tcaccacgga acgggcagat gataaagccc
      901 ggttcgaggc cgagcatttt caccagccgg tcagccatca gcccggaaca gctaatcagc
      961 gtcgatgctt catattcacc gccctgacgg gtgcgtatca ccacgccgtt tttatgctta
     1021 ctgagggcgc tgacttcggc gttataaata atctcgccgc ctctggcctg gaagattttt
     1081 gccattgccg ctgtgacttc gcggtagctg acaatgccgc tggacggcac aaaaatgcca
     1141 ccaagcccgg tgatattcgg ttcgcgctcg cgcagttcca cggcgtttat ccactcgcgc
     1201 tcgataccgt tcgccgctgt gcgttcccat aaggcgcgca tccgttccat ttcgagctga
     1261 gacgtggcga ccagcatctt gccgcagttg tcatagcgaa tgccgttttg atcgcaaaag
     1321 gctttggtgg cgcggttccc cgccaggcaa aactgcgcct tcaggctgcc gggcgtgtaa
     1381 tagaccccgg catggatcac gccgctgttg tggcccgtct ggtgacaggc cgggccggac
     1441 tctttttcca gcaacgcaat gcgggcatcc ggataaacat caatcagttg catggcggtc
     1501 gacatgccga tgatgccgcc gccaataatc acaaaatcat acatccgctc aattcctttg
     1561 cgcttactga tgcgtctggt agtggtgagt ggcgtaagcg aaatagccac gctgacgcat
     1621 cagttcacgg cgcagatccg ggtgcggagt aaagcggtcg cgaccgtgca gccagaacag
     1681 gttgtttatc aacaggaatt tgccaacggg aacgggtaca gaaagaatgc ctttgctggt
     1741 ttcaatggca tcggaaagct cgctcaacca cacgccttct tcaaaatctt ttggctggac
     1801 gaactggtcg atatagcgca tcaccgggcg accctgttga tcaacgtcga acactggatg
     1861 gaaaacatct ttgctgacgt ttttgctcgg cggcgcggca aagcgcatcg ggcgacgcgc
     1921 cagtggatgg cggaaatagt ggtccagatg ttcccagtca tcgagatgca gcagcaacga
     1981 atttccaccc tgcatgtttt gctcatcgat tttcatcatc agcacgtaat cggtgatctc
     2041 ttcgacatac gtgccgtcgt tgtgcagttc catcacgcgg tgcggctgac gcagatagct
     2101 gtctgagtta tcgacatttt tcaccacgaa acgcgcgtaa tactgaccgc tcatcgcatc
     2161 gaaatttgag cgaccaatca gatgcgctac cgccgttgcc agcttcacca tctcatctgc
     2221 ctgcgccaca tcatcgatac ccaccgcatt gatcaacagc gcaccttcag cacggtttag
     2281 cagggtcttc aacagcaatg gttgcagctg attcgcacac agatcgtcaa gaattttgcc
     2341 tacccgaaaa cgcagaaacg atttgtactc cagcgcctgt acgggccatt cggcaacctg
     2401 ctcgagaaac tgtttggtcg tctgttcggt gaaggtgagt tccagcagac gcggggattg
     2461 cgccgacggg atgagagtga atccgctata gtcctggcct gaatcgacag cgttattttg
     2521 tacggcggtc agtgcattca tcagaagcga tcctcttatg agatgtaggg tgacatggcg
     2581 atgctcattt cgtagccata atctaaaaat atctacattt ctgaaaaatg cgcacaaaag
     2641 cgacatattg ttttcttatt gtgatcaaaa gcaacaaatt agtaacattt aacgtagagt
     2701 attaaggcaa agtgagacag gaaatgaata actaaagcaa caggttaggg ggagttaaca
     2761 ttagagaaca gattatctgg tcgataaaaa acagtaaatg gcttcactgc aaaatgaccg
     2821 ttagtaaact aaacatatta tctgccccgt tttatcgggg cagatataac attaaattat
     2881 aaatagagat agttcggcgg ttctcctgtt ttccctaaaa gaagagcgct acgcccagga
     2941 atattgatat ttagagttcc gcctctaagc gtaaatactt taccaggggg gataattttt
     3001 cgggtattat taataataat gttaacgcta ttactcccca tgtttttata tattccaggt
     3061 tgcaatttgt taggccaggt accttcaact tcttcgtttc ctttatttat tataactagt
     3121 ccatcatgag gagagtgatt aatgttcaca gaatccccac gactaaaggc tagaacgttt
     3181 ttagttgcaa atacaccctc aaatgcgctc catattcctg ctttatctat atataatgcg
     3241 tggaattcat tgtagagctg ctgataggta gagcaacctt tattaagcca cgcctcacca
     3301 ccgacacgcg tggacccatc ataattttta atttcagtcg ttgattttaa tacctggcca
     3361 tcccagtaaa ctaaaggtct gctgaacggg agagcggcca tatatgccat tgctaattgt
     3421 tcatcttgtt gatggttaaa tagaaagtca ttgaaaagat tgccattgtt aggaaaatcg
     3481 tggttaatgt taatcaatat ccgtctttct gatgggatgc ttgagattgt ggaacccggt
     3541 tttgatgcaa gggttaggag atctcccccg aatgaaaagg cttttatcgt cttcatgagc
     3601 aatgaaaaat caatggcgct taaatcatct ctttcataaa gcagtgatga catttgttgt
     3661 tcatccaact gacaagttac cagatattcc atgtaattca taacggcttt tttatgatat
     3721 tttgggttaa ataagttagt attgtgtaac cgctcattat aatttttaag cggtggggtt
     3781 attgatcttt ctatttgttc atgttttgag tgttttgccg catcataacg taaacctcgt
     3841 acgccatatt ttcgcatttc acgaacgtat tgattttgca acataataac ctggtcactt
     3901 ttgggtgttg ttttaggaat tccatttaaa cgcttttctt ttaatgcatg gaggttttcc
     3961 cattcatcct tagagatgtt atctttactt gcttcgaagt cttttgcagt gagatgtata
     4021 tcttctggat ggatttgttt acgttctttt gttactggat ctttaatgaa tggaatcaaa
     4081 acctgacctt gccaatagcc agggatacca ccttctatat ctttactgat cttcgctcgt
     4141 atatcagcag agggataatc caggtcattt ttaccactac ccccaattcc cataaagtta
     4201 aaggtataat caggaatgat atcgatacca aattttttag catctttaac caatgcaata
     4261 aaatcctgca aatctcccat tggattatgg atggtacaaa gatcttcggg ttgataagca
     4321 tgaaaccagt aacccttttc ttcttcggga gtttctggtc cgtcggcgcg gaaagttgtc
     4381 actggaggga tttgtatata cccataatgg caacgtgcaa actcagacat attttccctg
     4441 atttgctggt aggtggcatt aaaagcatga agaatcgctt cgccgggttt tttaggggtg
     4501 atgatttcac attgtgattt ctgaacagca gtaagagatt tacgctcaag tgtctgccgt
     4561 agatgttctg gattgattag tattttaaat gtttcttgct gtccggaagg tgacgtaacg
     4621 ttaatattat atacat
//
LOCUS       DAJTZL010000235         4627 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.236, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000235 DAJTZL010000000
VERSION     DAJTZL010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4627
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.236"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(149..1231)
                     /locus_tag="OO544_003858"
     CDS             complement(149..1231)
                     /locus_tag="OO544_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HCR6769346.1"
                     /translation="MNLPVVPPLNVIRERLQLIFPEGTENRGYLIREIAAKTIFVMFY
                     AGAVEGLGRWIRPSQVVSMGDSQAALTDDASRESWVKMTFSKQKIRPADAWYAENTRE
                     PIRDETIKNGLIPCNAIVERKGIPTTSSRPRYSLNRSFAALFDAGLEGDSLLSAISSW
                     QENHLNKAALARLRLLKSGAVIAADAVVVTFPNGEKRTLAPGPSSTIAKAVIEVFAPN
                     FLKNPTVLWLSESGNKVVAQDDVLAKALGLNIDASKALPDIILIDLGEDRTGSDMLVV
                     FTEVVATDGPINRERKVTLTHLAIDAGFSEKNLAFLTAFMDRSALPFKKAIPELAWGS
                     YAWFASEPEHLIDLRDGYPVKISQRK"
     gene            complement(1228..3024)
                     /locus_tag="OO544_003859"
     CDS             complement(1228..3024)
                     /locus_tag="OO544_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020803211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HCR6769347.1"
                     /translation="MNVQHFDFFTDNEFSNLERYQTCKAMAKGYAGNYKTGQSRLMHA
                     RSYCFSIVAAYWDTLAKKQKSSIKMRAVPHTVYIEDIPCDARELAERTGELIAQFPAE
                     DAGYLIGSIYTVMLPSAYRSELGAYYTPPPLVARLLDLAEKSGVDFSHASVIDPACGG
                     GAFLAPVAIRMLKKDKGSSPEWMLRRISRRLKGIEIDPFAAWMSLVLLESVLMPLCVK
                     VKRRLPEDTIIVADALQQDKFSGYDLVVGNPPYGRVTLDIKTREKYSRSLFGHANLYG
                     LFTDLAVRMVKEKTGVIAFLTPTSFLGGQYFTALRTLLTEKTTPYALDFVTDRDGVFD
                     DVLQETMLTAFKAGEHKVRAQVSSLVPKGLNKAKIEKIGNVDIEKGGATWLLPRVKDD
                     ACFLNVLKQMSTRLADLGYSVSTGQLVWNRFKSQLRTTKGKNSYPLVWAESITSAGFR
                     FSADRKNHVPYIDITPQQGFLVTKSECVLVQRTTSKEQDRRILAAILPQGFIDETGGV
                     VVENHINIVYSNGLFSAVRPDVIDMLLNSHVVDRAFRCISGSVAVSAYELNSIPLPSL
                     EQVMEIQSLIDAGVHRRIIERTIAGFYGVMIA"
     gene            complement(3275..3631)
                     /locus_tag="OO544_003860"
     CDS             complement(3275..3631)
                     /locus_tag="OO544_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6769348.1"
                     /translation="MEIKPEDELSNIVLFPVKEDNPRNQVNFLYEPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEERERRKNIEKLIQIE
                     RNAKARIRRVTKYSPE"
     gene            complement(3689..4111)
                     /locus_tag="OO544_003861"
     CDS             complement(3689..4111)
                     /locus_tag="OO544_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HCR6769349.1"
                     /translation="MAKVFTQEEREKIKGQVVELVRQSGRETLRQLEAKTGATRYLIS
                     VLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKLKKPSVVDPDLIWSLPDG
                     EIRRYDRHQNIICRECRKSEVMQRVPAFYQGDFQEAVL"
     gene            complement(4127..>4627)
                     /locus_tag="OO544_003862"
     CDS             complement(4127..>4627)
                     /locus_tag="OO544_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HCR6769350.1"
                     /translation="IEQHGPQTADELALMFGITSRRANSSLAMAISKGRLIRVNQGGK
                     FRYCIPGADLPAEPKAASIAETDGKVFPQPAGVALPVQEAATQEEIKTETVADIVQSL
                     PSFTETQADDLVLPSLHMANRELRRAKSHVQKWERVCAALRELNKHRDIVRQIVDSSG
                     RIVSEK"
BASE COUNT         1240 a         1200 c          909 g         1278 t
ORIGIN      
        1 cacagaacaa ttattgcttt tgccgttgat gacaataaag tttttatcgc aggcgtatac
       61 cacggtgggc aaatctatga aaccgatttt ttatccagtg caccgtaaaa cccccatcct
      121 tcagggcgtg gcggatatca aactttggtc attttctctg gctgatcttt accggataac
      181 catcccgaag atcaattaag tgctccggtt cagaagcaaa ccatgcataa gatccccacg
      241 ccaattctgg tattgccttt ttaaaaggca gagcacttct gtccataaat gccgtaagga
      301 atgcaagatt tttctcgctg aacccagcgt caatagccaa atgagtgaga gtaacttttc
      361 gttccctgtt tataggccca tctgtcgcaa ctacttcagt gaaaactaca agcatgtccg
      421 aacccgttcg gtcctctccc aaatcaatca aaataatatc tggaagtgct tttgaggcat
      481 caatattcag ccccaatgct tttgccaata catcatcctg agcaacaact ttattcccgg
      541 attcagacaa ccagagcaca gttggattct taagaaaatt aggagcaaaa acttcgatga
      601 cggccttagc tattgtactt gacgggcccg gagctaaagt tctcttctct ccatttggga
      661 aagtgacgac tacggcatca gcagcaatca ctgcccctga tttcaataaa cgcaacctag
      721 caagagcggc tttgttcaaa tgattttctt gccaggaact tattgcactc agcagcgaat
      781 ctccttcaag cccggcatca aagagtgctg caaaagagcg attcaaactg taacgaggac
      841 gagaggatgt ggtgggtata cctttacgct caacaattgc attgcaaggt attaggccat
      901 tttttatagt ttcatcacgt attggctcac gcgtattttc cgcataccat gcatcagcag
      961 gccttatctt ttgtttagag aaagtcatct ttacccagct ttctctggac gcatcatcag
     1021 taagagcagc ctgcgaatct cccatactta caacctggct gggccttatc catcgcccca
     1081 gcccctcaac cgcaccagca taaaacatga caaaaattgt ttttgcagct atttcccgaa
     1141 ttaaatatcc acgattctca gtcccttcag ggaaaattaa ttgtaatctt tcccggataa
     1201 cattcaatgg gggaacaaca ggcagattca tgcaatcatc actccataga aaccagcgat
     1261 agttctctca ataatccgtc tgtggacgcc agcatcaatt aacgattgaa tctccatcac
     1321 ctgctccaga gaaggcaacg ggattgaatt caattcatag gccgaaacag caacacttcc
     1381 gcttatacac ctgaacgccc gatcgacaac atgcgaatta agtagcatat caataacatc
     1441 agggcgaacc gcagagaaca atccgtttga atatacaata ttaatatggt tttcaactac
     1501 tacgccacca gtttcatcta taaatccctg aggtaaaatt gccgctaaaa tccttctgtc
     1561 ctgttccttc gatgttgtcc gctgaacaag cacacattca gattttgtca caaggaaacc
     1621 ctgttgtggg gttatatcga tatagggaac atgatttttt ctgtctgcac taaaacgaaa
     1681 accagctgaa gttattgatt ccgcccagac taatgggtag ctattttttc ctttcgtcgt
     1741 acgcaactgc gacttaaatc gattccagac taactgacct gtagagactg agtatcccag
     1801 atcagctaaa cgcgtagaca tctgttttaa tacattaaga aaacaggcat catctttaac
     1861 gcgtggcaaa agccacgttg ctccaccttt ttctatatca acattaccta ttttctcaat
     1921 ttttgctttg ttcaacccct tagggacaag tgaggatacc tgggcccgaa ctttatgttc
     1981 tcccgccttg aatgctgtca acattgtttc ctggagaaca tcgtcaaaga ccccatccct
     2041 gtcagtgacg aaatcaaggg catacggagt agttttttcg gtcaaaaggg ttcgcagcgc
     2101 ggtgaaatac tgcccaccaa gaaaagaagt tggcgtaaga aatgcaataa ctcctgtttt
     2161 ctctttaacc atgcgaacag ctaagtcggt gaacaatcca tatagatttg catgaccaaa
     2221 taacgaacga gaatattttt ctctcgtttt aatatcgaga gtgacacgtc cgtaaggcgg
     2281 attccctacc acaagatcat aaccagaaaa tttatcttgc tgaagcgcat cagcaacaat
     2341 aattgtatct tcaggcaatc tacgttttac ctttacacat agtggcatta gtacagattc
     2401 gagcaatacc aaactcatcc aagccgcaaa tggatcgatc tctatcccct ttaaacgacg
     2461 actgattctc ctcaacatcc attcagggga agaccccttg tcttttttta acattcgaat
     2521 ggctactggt gccaaaaatg caccgccacc acacgcagga tcaataaccg atgcatgaga
     2581 aaaatcaaca ccagactttt cagccaggtc caagagacgc gctaccaaag gtgggggggt
     2641 atagtaagcc cccaattccg aacggtaagc ggatggcagc attaccgtat aaattgaccc
     2701 aatcagatat ccagcgtcct ccgccggaaa ttgagcaata agctcacctg ttcgttcagc
     2761 gagctcgcgt gcatcacatg gaatgtcttc tatgtaaaca gtgtgcggta ctgccctcat
     2821 tttaatgctg cttttttgct tttttgcaag ggtatcccaa tatgcagcta cgatgctgaa
     2881 gcagtatgag cgggcatgca tcaaccttga ctgcccggtt ttgtaattgc ccgcataacc
     2941 tttggccatg gctttacaag tttgatatct ttccagatta gaaaactcat tatctgtaaa
     3001 aaaatcaaaa tgttgcacat tcataatgta aaatcctaat gtatcaaaca tgcgggccat
     3061 aacatctcat ggtggcgcat agcaaactgg cccgaagaat atcacgcacc aagtcgatct
     3121 cataacccgt tatgacaata aatctttgta gctgaactca cttggtgatt tgtcaatcat
     3181 tcctggcctc cagttcgtcc tgaatctctt tatcgagttc accgtatttg ttagtcctca
     3241 tcactcacga cttgacttcc agaaataacc ttgattattc ggggctatat tttgtcaccc
     3301 tgcgtatccg cgctttcgca ttacgctcaa tctggattag cttttctata ttttttcgcc
     3361 tttcccgctc ctcctggcgc aagagcctta catcatctgc cagtctggtt tctcttttcg
     3421 ccacagagag catccagtca aatggctcca caactgcacc gcaaatttta cagcggacct
     3481 gacgctcttt ttcgtcaacc cggacagaag cgtgatggca atatggtctt tccgatggct
     3541 cataaagaaa attaacctga ttacgagggt tatcctcttt taccggaaat aaaacaatat
     3601 tgcttaactc atcttctggt tttatttcca tgctcctctc ctttgatgcg aatgccagcg
     3661 gtaattgaag cctgatagct aatttcactc acagtaccgc ctcctgaaaa tcaccctgat
     3721 agaaagccgg tacacgctgc ataacttcac tcttccggca ctcgcgacag attatgttct
     3781 gatgtctgtc gtagcggcgt atttctccgt ccggtaatga ccagataagg tccggatcaa
     3841 ccacagatgg tttcttcagc tttgccctcg agagtttttt gcgggcgttt tgccagtcct
     3901 tacgagcctg ttcagacggg aataacccgt agccagagtt gtatacatcc ccactggcaa
     3961 ccagctctct ggcgagaacg cttattaaat atcttgtcgc cccggtttta gcttccagtt
     4021 gtcgtaacgt ctcgcgccca ctctggcgta cgagttcaac aacctgcccc ttaatttttt
     4081 cccgctcttc ctgtgtaaat acttttgcca taagcgcccc cggcaatcac ttttccgaca
     4141 caatacgacc ggaggaatcg acaatctgtc gaacaatatc ccggtgcttg ttcagctccc
     4201 gcaacgcggc gcagacacgc tcccacttct ggacatgact tttcgcccga cgcagttcgc
     4261 ggtttgccat atgcagcgat ggtaaaacca ggtcatccgc ttgcgtttcg gtgaacgatg
     4321 gcaacgactg cacaatgtcc gccacagttt ctgttttaat ttcttcctgt gttgcagctt
     4381 cctgtaccgg taacgcaaca cctgctggct gaggaaagac tttaccatcc gtttccgcta
     4441 tggatgcggc tttcggctct gccggtaaat cagcgcccgg tatgcagtac cgaaatttac
     4501 cgccctgatt cacgcgaatc agacgccctt tgctgattgc catggccagc gatgaattcg
     4561 cccggcggga ggtaatcccg aacatcagtg ccagctcatc cgccgtttgt gggccatgtt
     4621 gttcaat
//
LOCUS       DAJTZL010000236         4608 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.237, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000236 DAJTZL010000000
VERSION     DAJTZL010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4608
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.237"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..2163
                     /locus_tag="OO544_003863"
     CDS             <1..2163
                     /locus_tag="OO544_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HCR6769351.1"
                     /translation="KGTIAWSILSSHNTSGNMDKLKIKFDSLASHDITFVGIVQTAKA
                     SGMERFPLPYVLTNCHNSLCAVGGTINGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMR
                     EMMAGGGKMILGSDSHTRYGALGTMAVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGK
                     PAPYVGPQDVALAIIGAVFKNGYVKNKVMEFVGPGVSALSTDFRNSVDVMTTETTCLS
                     SVWQTDEEVHNWLALHGRGQDYCQLNPQPMAYYDGCISVDLSAIKPMIALPFHPSNVY
                     EIDTLNQNLTDILREIEIESERVAHGKAKLSLLDKVENGRLKVQQGIIAGCSGGNYEN
                     VIAAANALRGQSCGNDTFSLAVYPSSQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCF
                     GAGDTPINNGLSIRHTTRNFPNREGSKPANGQMSAVALMDARSIAATAANGGYLTSAS
                     ELDCWDNVPEYAFDVTPYKNRVYQGFVKGATQQPLIYGPNIKDWPELGALTDNIVLKV
                     CSKILDEVTTTDELIPSGETSSYRSNPIGLAEFTLSRRDPGYVGRSKATAELENQRLA
                     GNVSELTEVFARIKQIAGQEHIDPLQTEIGSMVYAVKPGDGSAREQAASCQRVIGGLA
                     NIAEEYATKRYRSNVINWGMLPLQMAEVPTFEVGDYIYIPGIKAALDNPGTTFKGYVI
                     HEDAPVTEITLYMESLTAEEREIIKAGSLINFNKNRQM"
     gene            complement(2397..3680)
                     /locus_tag="OO544_003864"
     CDS             complement(2397..3680)
                     /locus_tag="OO544_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HCR6769352.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNMYYGFLAVNSRFN
                     AFGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(3815..4507)
                     /locus_tag="OO544_003865"
     CDS             complement(3815..4507)
                     /locus_tag="OO544_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6769353.1"
                     /translation="MLNGYIDEGKAASAKLIRSTLSDAFREAIAEGHITTKPVAATRA
                     AKSEVRRSRLTADEYLKIYQTAESSPCWLRLAMELAVVTGQRVGDLCEMKWSDIVDGY
                     LYVEQSKTGVKIAIPTTLHVDALGISMKETLDKCKEILGGETIIASTRREPLSSGTVS
                     RYFMRARKASGLSFEGDPPTFHELRSLSARLYEKQISDKFAQHLLGHKSDTMASQYRD
                     DRGREWDKIEIK"
BASE COUNT         1094 a         1142 c         1194 g         1178 t
ORIGIN      
        1 aaaggcacta ttgcctggtc tattctctct tctcataata cgtccggaaa tatggataaa
       61 cttaaaatta agtttgattc attagcctct cacgatatta cctttgttgg tattgtacag
      121 accgctaaag cgtccggaat ggaacgtttc ccgctgccgt atgtgctgac caactgccat
      181 aactcactct gcgccgtcgg cggcaccatt aacggtgatg accatgtttt tggtttatcg
      241 gcagctcagc gttatggcgg tatttttgtg cctccgcata ttgcggtcat ccatcaatat
      301 atgcgtgaga tgatggcagg cgggggcaaa atgatcctcg ggtcagacag ccacacccgt
      361 tacggtgcat tagggacaat ggcagtcggt gagggtggcg gtgagttggt aaaacagctg
      421 cttaatgaca cctgggatat cgactatccg ggcgtggttg cggtgcatct gaccggaaaa
      481 cctgcgccgt atgtggggcc acaggatgtg gcgctggcta tcattggcgc ggtgttcaaa
      541 aacggttacg tcaaaaacaa agtcatggag ttcgttggac cgggcgttag cgcgctctct
      601 accgatttcc gtaacagcgt tgacgtgatg accacggaaa ccacctgttt aagctccgtc
      661 tggcaaactg atgaagaagt gcataactgg ctggcgctgc acggtcgcgg ccaggattac
      721 tgccagctta accctcaacc gatggcgtac tacgatggct gcatcagcgt tgatttaagc
      781 gccatcaaac caatgattgc gctgccgttc cacccgagca acgtgtatga aatcgacaca
      841 ctgaaccaga acctgaccga cattctgcgt gagattgaaa ttgagtccga acgcgtggcg
      901 cacggtaaag ccaaactctc gctgctggat aaagtggaaa atggtcgcct gaaagtgcag
      961 caggggatta tcgcgggctg ttctggcggt aactacgaaa acgtcatcgc ggcggcgaat
     1021 gcactgcgcg gtcaatcctg tggcaatgac accttctcgc tggcagttta cccgtcatca
     1081 cagccggtgt ttatggatct cgccaaaaaa ggtgtggtag cagatttgat tggcgcaggc
     1141 gcaatcatca gaaccgcgtt ctgcggccca tgctttggcg cgggcgatac gccaatcaac
     1201 aacggtttga gtattcgcca caccacgcgt aacttcccga accgcgaagg ctctaagcca
     1261 gctaatgggc agatgtcagc ggtggcgttg atggacgctc gttctatcgc tgcgactgcg
     1321 gcaaacggtg gctatttaac ctctgccagc gaacttgatt gctgggacaa cgtgccggag
     1381 tacgccttcg atgtaacgcc gtataaaaac cgtgtttatc agggctttgt gaaaggggca
     1441 actcagcaac cgctgattta cgggccgaac attaaagact ggccggaatt gggtgcgctg
     1501 actgacaata tcgtcctgaa agtgtgctcg aagatcctcg acgaagtgac caccaccgac
     1561 gaactgattc cttccggtga aacctcttct tatcgttcaa atccgattgg tctggcggag
     1621 tttaccctgt ctcgccgtga tcccggttat gttggcagaa gtaaagcgac tgctgagctg
     1681 gaaaatcagc gtctggcggg gaatgtcagc gagctgacag aggtgtttgc gcgcattaag
     1741 cagattgctg gtcaggagca tattgatccg ctgcaaactg aaattggcag catggtctat
     1801 gcggtgaaac caggcgatgg ttctgcgcgt gaacaggcgg cgagctgcca gcgtgtgatt
     1861 ggcggtctgg cgaatattgc cgaggagtac gcgactaaac gctaccgttc taacgtcatc
     1921 aactggggga tgttacctct gcagatggcg gaagtgccaa cctttgaagt gggggattac
     1981 atttacatcc ctggcattaa agcggcgctg gataatccgg gtacgacgtt taaaggttat
     2041 gtgatccatg aagatgcgcc ggtaacggaa attacgctct atatggaaag tctgactgct
     2101 gaagagcgcg agattatcaa ggcgggtagt ttgattaact tcaataaaaa ccgtcagatg
     2161 taaaaagcgc catgtgaatg taggtcgcat tcggcactta ttgtcggatg cgatgcttgc
     2221 gcatcttatc cgacctacga atcgcatcga atctgtaggc cagataaggc atttacgcag
     2281 catccggcac ttattgtcgg atgcgatgct tgcgcatctt atccgaccta caaatcgcat
     2341 cgaaccgtag gccggataag gcgtttacgc cgcatccggc aaatagttaa ttgctcttac
     2401 ttcttcgcct ctgcaaccac tttactaccc acgccgcggt tattgtattc ccacatgcgg
     2461 ttgtagttag tgtcattcag attgcgctgt atttcgtcgt tatcatctac gctgccggta
     2521 ttacccgcaa acggacgatt agagatcacc gcatcggccc acggtttagc cgtgttaaaa
     2581 ccttcgttga tggcgctatc acggatcacc acctgaccgt tggtattggc atcaacatcc
     2641 agcgagcggc ccagttgcgc cacaccatca ccgaaagcat tgaaacggct gtttacggcg
     2701 aggaaaccgt agtacatgtt ggacagcgta gccggtgcaa acacatacgc ttcttgctga
     2761 gtacgtgagt tcaccacgcg gaattcggtg ttatcgaaca ccactgcgcc gcgaccagaa
     2821 acgatatcca catccccttc aatgtagctg ttggtcacca gcgtacgcgg ctgacgattc
     2881 gtttccagac ggttctgcac accgctgttg gtgacaaaga aggtgttctg acgaccgaga
     2941 atgttaacgt tgttaatctg tacctggtca ccatcagtac gcagtgccac cgccggatgg
     3001 ttacctgcat ctacgctatc gcccagcgtg ttttcgatgg tcagattttg cagttgcagg
     3061 ccattgtttt gtgaccagaa gaccgcagag cagagaacac cgatactgtc gctgcgtttg
     3121 ctctggcagc tatcgtacat ataccacgct ggtttacctg gcatatattt gccgcgcggg
     3181 ttgacgtcgt gacgccagtc ggcagggctc atgccaccat caagggaaag cccaatcttc
     3241 acatcaatcg gtttttcacc tgtaccgtac agagtaattc cacccggagc ggcagggaca
     3301 tataccgttc cctgatactc accaggcatc acggcaatat actggcgctt gttggtacgc
     3361 ttgataattg ccgcatctac cgccgcctga atcgtggtat gcgttacacc ttgagtgccc
     3421 gccgggccga caacaaagtc aggttgcgca ggcagggtaa tcggggaagg attccacgct
     3481 gcagcacctg gtgtcaggga tgcaaaatag tgttgagcat cgaaattctg cgcttctttt
     3541 gccgacagaa tcgggcgaga agaggtacca ggcgcggttt gatcagaagg acgttgatcg
     3601 ggcggggttg agctacaggc ggtcagcgtc acgccaaaag ccaatgccag cgccagacgg
     3661 gaaactgaaa atgtgttcac aggttgctcc gggctatgaa atagaaaaat gaatccgttg
     3721 aagcctgctt ttttatacta agttggcatt ataaaaaagc atcgcttatc aatttgttgc
     3781 aacgaacagg tcattatcag tcaaaataaa atcattattt gatttcaatt ttgtcccact
     3841 ccctgcctct gtcatcacga tactgtgatg ccatggtgtc cgacttgtgc ccgagaagat
     3901 gttgagcaaa cttatcgctt atctgcttct catagagtct tgcagacaaa ctgcgcaact
     3961 cgtgaaaggt aggtggatcc ccttcgaagg aaagacctga tgcttttcgt gcgcgcataa
     4021 aataccttga tactgtgccg gatgaaagcg gttcacgacg agtagatgca attatggttt
     4081 ctccgccaag aatctctttg catttatcaa gtgtttcctt cattgatatc ccgagagcat
     4141 caacatgcaa tgttgtaggg atggcaattt ttacgcctgt tttgctttgc tcgacataaa
     4201 gatatccatc tacgatatca gaccacttca tttcgcataa atcaccaact cgttgcccgg
     4261 taacaacagc cagttccatt gcaagtctga gccaacatgg tgatgattct gctgtttgat
     4321 aaattttcag gtattcgtca gccgtaagtc ttgatctcct tacctctgat tttgctgcgc
     4381 gagtggcagc gaccggtttt gttgttatat ggccttcagc tattgcctct cggaatgcat
     4441 cgctcagtgt tgatctgatt aacttggctg atgccgcctt gccctcgtct atgtatccat
     4501 tgagcattgc cgcaatttct tttgtggtga tgtcttcaag tggagcatca ggcagccccc
     4561 tccttattgc tttaattttg ctcatgtaat ttatgagtgt cttctgct
//
LOCUS       DAJTZL010000237         4595 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.238, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000237 DAJTZL010000000
VERSION     DAJTZL010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4595
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.238"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(98..856)
                     /locus_tag="OO544_003866"
     CDS             complement(98..856)
                     /locus_tag="OO544_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCR6769354.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLTVQLGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFAHPKSERTKAFLSQVIH"
     gene            complement(864..1967)
                     /locus_tag="OO544_003867"
     CDS             complement(864..1967)
                     /locus_tag="OO544_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCR6769355.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAVWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTNIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(1977..3158)
                     /locus_tag="OO544_003868"
                     /pseudo
     CDS             complement(1977..3158)
                     /locus_tag="OO544_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026209.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
     gene            complement(3226..4251)
                     /locus_tag="OO544_003869"
     CDS             complement(3226..4251)
                     /locus_tag="OO544_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6769356.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPGFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTDYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
BASE COUNT         1269 a         1191 c         1085 g         1050 t
ORIGIN      
        1 gggtctcccc atgcgagagt agggaactgc cagacatcaa atcaagcgaa aggccatccg
       61 aaaggatggc ctttttgctt ttcgaactaa cattcaatta atggattacc tgcgataaaa
      121 atgccttcgt acgctctgat ttaggatgcg caaaaaattc atcaggtgca gcttgctcca
      181 ctatttcccc acgatccata aaaatcaccc ggtcagcgac ggttcgcgca aaccccatct
      241 catgcgttac acacaacatt gtcatacccg attgcgccag cccaatcatc gtatccagca
      301 cctctttcac catctccgga tcgagcgccg acgttggctc atcaaacaac ataattttcg
      361 gcttcataca cagcgaacgc gcaatggcaa cgcgttgctg ctgaccacca gaaatttgtc
      421 cgggaaactt atgcgcatgt tcggcaattc tcacccgctc aaggtaatgc atcgccagag
      481 cttcagcctc tttctttggc atcttgcgta cccaaatcgg tgccagggta cagttctgta
      541 gaacggtcag atgagggaag agattgaaat gctgaaagac cattcccact tcctgcctga
      601 cgcgctcaat attgcggata tcttcattaa gttcgatgcc atctactacg atccgtccct
      661 gttgatgttc ttccagatga ttaatacaac gaatggttgt cgatttaccg gaaccagaag
      721 ggccacacag aacgatccgt tctcccagtt gcacggttaa atttatattt ttcaaaacat
      781 ggaattgtcc ataccattta ttgacgtttt ccagcgtaat catcgcgtta gcaggttgca
      841 gtaaaatttg gctcatagtg tcctcaatgc ggtgtacgcc cggtgttaaa acgtttttcc
      901 agatgctggc tatagcgcga catgctgaaa cagaagatcc agtagatcag tgcggcgaaa
      961 acataccctt ccgtcgacat acccaaccag gcgggatcaa cggttgcctg ctgaacgcta
     1021 ctgaaaagat cgaacaaccc gataatgatc accaggctgg tatctttgaa gagtgcgatg
     1081 atggtattta ccagcccagg aattaccagc ttcaacgcct gtggcagaat aaccagcccc
     1141 tgagttttcc agtaacccaa tgccagtgac tctgccgctt catattgccc tttaggcagc
     1201 gcctgtaatc cgcctcgcac gacttccgca acatatgctg actgaaacag gatcacgcca
     1261 accagcgcgc ggatcagttt gtcgatattg gtgccttctg ccataaacaa aggcagcatg
     1321 accgaagaca taaacaatac ggtaatcagc ggtacgccgc gccagaactc gataaaaatg
     1381 accgataaga tacgcacaat cggcatatgg gagcggcgac ctaacgccag taagatcccc
     1441 cacggtagcg cccccgcaat cccaacggat gcaataatta aagttagcgt cagcccgccc
     1501 cattgccggg tttcaacccg ctccagacca aaaaaaccgc catacatcag ccaccagaca
     1561 atcagtgggt aaatcaccgc ccagaccgca atatagcgac cgcgatgcgg gagtattttc
     1621 cagaacattg gtgcgattga cacaaggcca atcagtaatg ccaggttaat tcgccagcgt
     1681 tggtcgtgtg ggtaaagtcc atacataaac tgaccaaatc gttcgtggat gaagacccaa
     1741 caggcaccgg cttttgtgca gtcggcacgc gttgagccaa cccaattagc ctgcaaaaat
     1801 gcccagttca gcaacggtgg gatcagttcc cacatcaacc atatgcagcc aatagtcagc
     1861 aggctattgc tccagctgga gaacagattt tttcgcaccc acaccatcgc gcggcttgag
     1921 ttatggctcg ctgggcgcga gggctgagac agcaatactt ttgtcatcat gactccttag
     1981 cgttcaacga tcgcgatgcg gcggttatag atattcatca gcagcgagat agtcaggctg
     2041 ataatcagat agaccgacat ggtcatggct atcgtctcga tggcttgccc cgtctggttc
     2101 agcacggtgc cagcaaacag cgaaaccata tcgggatagc caatagcggc ggcaagagag
     2161 gagtttttga cgatgttgag atactggctg gttaacggcg gaataatcac tcgcagtgcc
     2221 tggggaataa tgacctagcg tagcgtaacc gggttgggta atcccagtga ccgagccgct
     2281 tcatgttgac cataaggcac tgcctggatc ccagcgcgga taatctcggc gataaatgca
     2341 gatgtataaa ccgaaagtgc cagcgttaag gctgccagtt caggaattaa aaccatcccg
     2401 ccgcggaagt taaagcctcg tagggctggg acatcccagt gtagtgctgc gccaaaaagc
     2461 cattgcgcca gtaaaggcaa accaatgatc aacactgcgg cgatcggcca ggtgcggcgc
     2521 agttgtccgg tctttatctg gtatgtttta ttaaagcgga atagcccaac agaaaggact
     2581 atagccataa caacagccag gataaacgca ataaatccgt ctcccagctg cggtgacgga
     2641 atataaagcc cacgattgct caaaaaggcc agatcaaacg cgctcacagc ctggcgtggt
     2701 ccgggcaaat tgcgcaacac ggcaaagtac cagaagaaga tttgcagcag cgggggaata
     2761 ttacggaaga tctcaatata aattgtggaa agctttcgta gcagccagtt atccgaaagt
     2821 ctcgccagac cgataaagaa gcccaaaaca gaagcgaaca caatacacaa tgcagaaacc
     2881 agtagcgtat tgagtaagcc gacaataaaa acgcgtccgt aggtgtcgcc ctgctggtaa
     2941 tcgatcaaat gctggacaat accgaagcca gcgccgcgat ccagaaaggc aaaacctgaa
     3001 gtaatgccac gattattgag attcgttaca gtgttgtgaa ataaccaacc aacaatgccg
     3061 acaacagcaa caacggcaag gatctggaat aaccaggcac gaaccgtagg gttggcaaaa
     3121 gagagtgagc ctttaacggt tgagcggcga tgagacatag aaacctcgga aagcataacg
     3181 ctgaagagag cgccgccgta gcgacgctcg ttacctcaca ccgacttaac gcacgggcgg
     3241 cgcgtactga ataccgccgt tattccagag attattttgc ccacgtttaa ttttcagcgg
     3301 gctttctgaa cctacgttac gctcgaaaat ttccgagtag ttacccacct gtttgatgat
     3361 gttataggcc catttattat ccagcttcag atccttgccg taatcgcctt ctttacccag
     3421 cagatgtgcc atatcaggcg ttgctggatt agccgctttt tcatcgacgt tctgggaatt
     3481 gatgcccatc tcttcagcat tcagcatggc gaaaagcgtc cagcgtacaa tcgagaacca
     3541 ttcatcatcg ccacgacgaa ctaccggccc aagcggttct ttagagataa cctccggtaa
     3601 gacaatccat tcagctgggt tgcttaattt gatgcgcagg gcatacagtt gcgactgatc
     3661 cgaggccagc gtatcgcagc gaccagattc cagtgccttc gctgattcgt cagagcgatc
     3721 gaaagtcacc ggtgtgtact tcatattgtt tgccttgaaa tagtcggcga cgttgagttc
     3781 ggtatctgta cccgcctgaa tacagacggt agcgccatcc agttctttcg cgctttttag
     3841 ccccgcttta tcgtgcgtca ggaagccaat gccgtcgtaa tcagtgacgc cagtaaatgc
     3901 cattcccatc ccggcatcgc gagatgaagt ccaggtcgta ttacgggaga gcaaatccac
     3961 ctcccctgac tgtaaagcgg tgaagcgttc ttttgcagtg agcggggtat atttcacttt
     4021 cgtgtcgtca ccaaatacag cagcggcaac accacgacaa acatctacat caatacctga
     4081 aaacttaccg tctgcatcgg cataagagaa cccaggtaat ccatcactga tcccgcattg
     4141 cacaaaacct tttttctgta cggcatcaag cgtcgcgcca gcatgcgcct gatttgcaac
     4201 ggcaagcagc acgctggcgg cagccagtgt ggctatcatc atctttttca taatgcttcc
     4261 tgtgaggcga aaattatcgt tataggacgt ttgggaaacg tcttttacct gtctgtggcc
     4321 atcctcttat aagcaaaagt agtgccatct tttgcgcacg atttcgcgtc gcaataagct
     4381 gagtgtagac aggaaataat aaaaacagcg ccccgcaatg gtgcgaaaat gaaacatgcg
     4441 ccacattttg gcgcaaaata tttacgcgtg ttttactaag cagaataatg attgttagcg
     4501 tgagtatatt tctggtggga ggtgtgaaag ttaaattgat gcgttatttc gaaagcagcg
     4561 aaataacgcg atataacatg ctaaggtaat gactc
//
LOCUS       DAJTZL010000238         4543 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.239, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000238 DAJTZL010000000
VERSION     DAJTZL010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4543
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.239"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..346
                     /locus_tag="OO544_003870"
     CDS             <1..346
                     /locus_tag="OO544_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HCR6769357.1"
                     /translation="VRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFP
                     SLEAGNIGYKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVPRQTE
                     VNRESSLQTLVE"
     gene            385..678
                     /gene="eutM"
                     /locus_tag="OO544_003871"
     CDS             385..678
                     /gene="eutM"
                     /locus_tag="OO544_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HCR6769358.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            785..1072
                     /gene="eutN"
                     /locus_tag="OO544_003872"
     CDS             785..1072
                     /gene="eutN"
                     /locus_tag="OO544_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HCR6769359.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPQGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            1084..2487
                     /locus_tag="OO544_003873"
     CDS             1084..2487
                     /locus_tag="OO544_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase EutE"
                     /protein_id="HCR6769360.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPPAAVHEMGVFASLDDAVAAAK
                     VAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQPVLLKNIDERGKGTVSRDWVGRDAAKIA
                     AAIGLNVPQETRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNGPRIAGLGLGGEGWTTMTITTPTGEGVTSVR
                     TFVRLRRCVLVDAFRIV"
     gene            2498..3334
                     /gene="eutJ"
                     /locus_tag="OO544_003874"
     CDS             2498..3334
                     /gene="eutJ"
                     /locus_tag="OO544_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HCR6769361.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQSVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGRRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTLAGNRRISLEEAEQYKRGHGEEIWPTVKPVYEKMVDIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFRKQFPALQVHLPQHSLFMTPLAIASSGREKAEGLY
                     AK"
     gene            3324..4511
                     /gene="eutG"
                     /locus_tag="OO544_003875"
     CDS             3324..4511
                     /gene="eutG"
                     /locus_tag="OO544_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HCR6769362.1"
                     /translation="MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGSVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQPRLPLIAIPTTAGT
                     GSETTNVTVIIDAVSGRKQVLAHASLMPDVAILDAALTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGKSLPKAVGYGHDLAARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRERFSQIGRALRTKKSDDRD
                     AINAVSELIAEVGIGKRLGDVGATSAHYGAWAQAALEDICLRSNPRTASLEQIVGLYA
                     AAQ"
BASE COUNT          973 a         1189 c         1436 g          945 t
ORIGIN      
        1 cgtccgtgag cgcgcaccaa agctggtggt tgatggcgag ttgcagtttg acgccgcctt
       61 cgtgccggaa gtggcggcgc aaaaagcgcc tgccagcccg ctacagggca aagccaatgt
      121 gatggttttt ccgtcgctgg aagccggaaa tattggctac aaaatcgcac aacgactcgg
      181 cggatatcgt gccgtcgggc cattgataca aggacttgcc gcgccgatgc acgatctctc
      241 tcgtggttgt agcgtgcagg aaattatcga gctggcgctg gtggcagctg tgccgcgtca
      301 gacagaagtg aaccgcgaaa gcagtttaca aacactggtt gaatgaacgg tcccgttctg
      361 gaccctgata aagaggaaag cacaatggaa gcattaggaa tgatcgaaac ccggggcctg
      421 gttgcactga ttgaggcttc tgacgcgatg gttaaagctg cgcgcgtcaa gctggttggt
      481 gttaagcaga ttggcggcgg cctgtgtact gccatggttc gtggtgatgt tgcagcctgt
      541 aaagccgcga ccgatgcagg tgccgcagcg gcacagcgta tcggcgagct ggtatctgta
      601 cacgttatcc cgcgtccgca cggcgacctg gaagaagtct tcccgatcgg cctgaaaggc
      661 gatagcagca acctgtaatc accctgtgtg acgtcgattc gggcgtcacg ctctctcctt
      721 tttcatttta ccttctgcgg agggtagggg gagcttttac ctgaaatacg ccacggaggc
      781 gggtatgaaa ctggcagtcg tcactggaca aattgtttgt accgtacgcc atcacggact
      841 ggcgcatgac aaattgctga tggtggaaat gattgatcca caaggtaatc ctgacgggca
      901 atgcgccgtc gccatcgaca atattggcgc gggaaccggg gagtgggtgt tactggtgag
      961 tggcagttcc gcccgccagg cgcataaaag cgaaacgtca ccggtcgatc tgtgcgtgat
     1021 tggcattgtc gatgaggtgg tgtctggcgg tcaggtaatt ttccacaaat aaggcagaac
     1081 atcatgaatc aacaggatat tgaacaggtg gtgaaagcgg tactgctgaa aatgcaaagc
     1141 agtgacacgc cgcccgccgc cgttcatgag atgggcgttt tcgcgtccct ggatgacgcc
     1201 gttgcggcag ccaaagtcgc ccagcaaggg ttaaaaagcg tggcaatgcg ccagttagcc
     1261 attgctgcca ttcgtgaggc aggcgaaaaa cacgccagag atttagcgga acttgccgtc
     1321 agtgaaaccg gcatgggccg cgttgaagat aaatttgcca aaaacgtcgc tcaggcgcgc
     1381 ggcacgccag gcgttgagtg tctctctccg caggtgctga ccggcgacaa cggcctgact
     1441 ttaattgaaa acgcgccctg gggcgtggtg gcttcggtaa ctccgtccac taacccggcg
     1501 gcaaccgtaa ttaataacgc catcagcctg attgccgcgg gcaacagcgt catttttgcc
     1561 ccgcatccgg cggcgaaaaa agtctcccag cgggcgatca cgctgctcaa ccaggcgatt
     1621 gttgccgcag gcgggccgga aaacttactg gttactgtgg caaatccgga tatcgaaacc
     1681 gcgcagcgtt tgttcaagtt tccgggcatc ggccttctgg tggtgaccgg cggcgaagcg
     1741 gtagtagaag cggcgcgtaa acacaccaat aaacgtctga ttgccgcagg cgctggcaac
     1801 ccgccggtag tggtggatga aaccgccgac ctcgcccgtg ccgctcagtc catcgtcaaa
     1861 ggcgcttctt tcgataacaa catcatttgt gccgacgaaa aggtactgat tgttgttgat
     1921 agcgtagccg atgaactgat gcgtctgatg gaaggccagc acgcggtgaa actgaccgca
     1981 gaacaggcgc agcagctgca accggtgttg ctgaaaaata tcgacgagcg cggaaaaggc
     2041 accgtcagcc gtgactgggt tggtcgcgat gcggcgaaaa tcgcggcggc aatcggcctg
     2101 aacgtgccgc aagaaacgcg tttgctgttt gtggaaacca ctgcagaaca tccgtttgcc
     2161 gtgaccgaac tgatgatgcc ggtgctgccc gtcgtgcgcg tcgccaacgt ggcggatgcc
     2221 attgcgctgg cggtgaaact ggaaggcggt tgccaccaca cggcggcaat gcactcgcgc
     2281 aacatcgaaa acatgaacca gatggcgaat gctattgata ccagcatttt cgttaagaac
     2341 ggaccgcgca ttgccgggct ggggctgggc ggggaaggct ggaccaccat gaccatcacc
     2401 acgccaaccg gtgaaggggt aactagcgtg cgtacgtttg tccgtctgcg tcgctgtgta
     2461 ttagtcgatg cgtttcgcat tgtttaagga gtaacagatg gcgcacgacg aacaatggct
     2521 caccccacgt ctgcaaacgg cggcaacgct gtgtaaccag acgcccgccg cgacggaatc
     2581 tccgctgtgg ctgggggtgg atctgggcac ctgcgatgtg gtgtcgatgg ttgtcgaccg
     2641 cgacggtcag tcggtggcgg tatgcctcga ctgggccgat gtggtccgcg acggcatcgt
     2701 ctgggatttc ttcggcgctg tcaccattgt tcgtcgccat ctcgacacgc tcgagcagca
     2761 attcggtcgc cgttttagcc atgcggcgac ttcatttcct ccaggtacgg acccgcgtat
     2821 ttccatcaac gtgctggagt ctgctggact ggaagtgagc catgtgctgg atgagccaac
     2881 cgcagttgcg gatctgctgc aactggacaa cgccggtgta gtggatatcg gcggcggcac
     2941 taccggcatt gccatcgtga aaaagggcaa ggtgacgtac tcggcggatg aagcaaccgg
     3001 cggacatcac atctctctga ctctcgccgg aaatcgccgt atttcgctgg aagaggcgga
     3061 gcagtacaag cgcggtcacg gtgaagagat ttggcctacg gtgaaaccgg tgtacgaaaa
     3121 aatggtggac attgtcgccc ggcatattga agggcagggc attactgatt tatggctggc
     3181 gggcggctct tgtatgcaac cgggcgtggc ggaactgttt cgcaaacaat tcccggcgtt
     3241 acaggtgcat ttaccgcagc acagtttgtt tatgaccccg ctggcgatcg ccagtagtgg
     3301 gcgagagaaa gcggaggggc tatatgcaaa atgaattgca gaccgcgctc tttcaggcgt
     3361 tcgataccct gaatctgcaa cgggtaaaaa catttagcgt tccaccggtg acgctttgcg
     3421 gtccgggctc ggtgagcagt tgcggacagc aagcgcaaac gcgtgggctg aaacatctgt
     3481 tcgtgatggc agacagcttt ttgcatcagg cagggatgac cgccgggctg acgcgcagcc
     3541 tggctgttaa aggcatcgcc atgacgctct ggccatgtcc ggtgggcgaa ccgtgcatca
     3601 ccgacgtgtg tgcagccgtg gcgcagttgc gtgagtcagg ctgtgatggg gtgatcgcat
     3661 ttggcggcgg ctcggtgctg gatgcggcga aagccgtggc gttgctggtg acgaacccgg
     3721 atagcacgct ggcagagatg tcagaaacca gcgttctgca accgcgcttg ccgctgattg
     3781 ccattccaac gaccgccgga accggctctg aaaccaccaa tgtaacggtg attatcgacg
     3841 cggtgagcgg gcgcaagcag gtgttagccc atgcctcgct gatgccggat gtggcgattc
     3901 tcgacgccgc gttgaccgaa ggtgtgccgt cgcatgtcac ggcgatgacc ggcattgatg
     3961 cgttaaccca tgccattgaa gcatacagcg ccctgaacgc tacaccgttt accgacagtc
     4021 tggcgattgg tgccattgcg atgattggca aatcgctgcc gaaagcggtg ggctacggtc
     4081 acgaccttgc cgcgcgcgag agcatgttgc tggcttcatg tatggcggga atggcgtttt
     4141 ccagtgcggg tcttgggttg tgccacgcga tggcgcatca gccgggcgcg gcgctgcata
     4201 ttccacacgg tctggcgaac gccatgttgc tgccaacggt gatggaattt aaccggatgg
     4261 tttgtcgtga acgctttagt cagattggtc gggcactgcg aactaaaaaa tccgacgatc
     4321 gtgacgctat taacgcggta agtgagctga ttgcggaagt tgggattggt aaacgactgg
     4381 gcgatgttgg tgcgacatct gcgcattacg gcgcatgggc gcaggccgcg ctggaagata
     4441 tttgtctgcg cagtaacccg cgtaccgcca gcctggaaca gattgtcgga ctgtacgcgg
     4501 cggcgcaata aatgccggat gcgacgcttg ccgcgtctta tcc
//
LOCUS       DAJTZL010000239         4481 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.240, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000239 DAJTZL010000000
VERSION     DAJTZL010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4481
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.240"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(7..1068)
                     /locus_tag="OO544_003876"
     CDS             complement(7..1068)
                     /locus_tag="OO544_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HCR6769363.1"
                     /translation="MTEPLKPRIDFDGPLEVDQNPKFRAQQTFDENQAQNFAPATLDE
                     APEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWQCLWCLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(1065..2462)
                     /locus_tag="OO544_003877"
     CDS             complement(1065..2462)
                     /locus_tag="OO544_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HCR6769364.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSVKWRMMCEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDTWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTVDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            2617..3615
                     /locus_tag="OO544_003878"
     CDS             2617..3615
                     /locus_tag="OO544_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR6769365.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLAAHNIAVRD
                     ELIVDGGYTHETALQAARELLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(3726..>4481)
                     /gene="ompG"
                     /locus_tag="OO544_003879"
     CDS             complement(3726..>4481)
                     /gene="ompG"
                     /locus_tag="OO544_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HCR6769366.1"
                     /translation="AEPSVYFNAANGPWRIALAYYQEGPVDYSAGKRGTWFDRPELEV
                     HYQFLENDDFSFGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGW
                     LSMYKFANDLNTTGYADTRVETETGLQYTFNETVALRVNYYLERGFNMDDSRNNGEFS
                     TQEIRAYLPLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNG
                     LSVSLEYAFEWQDHDEGDSDKFHYAGVGVNYSF"
BASE COUNT         1092 a         1228 c         1098 g         1063 t
ORIGIN      
        1 attgcattat ttttcgctgg gtgttttgcc tttttgcagt gtttctttca cctgaccgat
       61 aagctgacga cggaaatccc cgaggcgagg tttgtcatcg tcaatccacg gcagcgggcg
      121 gcaaagctcc atagctttaa tcccgagtcg tgccgtcaga agtcctgcac caatcccctg
      181 agctgcgcgg gtagacaaac gagcagcgag atcttgcgac atccagtcca tccccacttc
      241 gcgcaccagt tcgctggctc cggcaaaagc gatattcagc aataccagct taaacagacg
      301 caaacggctg taatacccca gttcaatgcc atacagcgtg gcgatgcgat taattaaacg
      361 cagattgcgc caggcgataa acgccatatc gaccaacgcc agcgggctga ccgcaatcat
      421 caacgttgat tccgccgccg aacggctgat ttcgcgccgc gcctgggcat ctaaaactgg
      481 ctggaccaaa tgcgcataca ggctgaccac ttcacggtcg ttttgcgttt catggattga
      541 ggcataccag cgttgcagcg ccggatgcga ctgatcaata cccgcctgct gcgccagttt
      601 ttcgcaaaat gcgcggcctt tgcccgtgcc gtggctatgc aataaatcac gcgcttcgtc
      661 gcgttcatgg gcgcgctgtc gcaagcacca taagcactgc cactctgtta ccacagaacc
      721 tacgccagcg ccgataatca atgccccagc ggcacatcca cccagcgcta cccagtcctg
      781 ggtttgccag gcattcattg tccactgtac accctgcccg acaacacttg cgccaaacag
      841 agccagcccg cccatcacca ttttgcgcca caggctgcgt ttcggacgta acgctgcatc
      901 cattaccgct tcaacttgcc cctcttcttc aggcgcttca tcgagcgtgg ccggggcaaa
      961 attttgcgcc tgattttcgt caaaggtctg ctgcgccctg aattttggat tctgatcgac
     1021 ctccagcgga ccgtcgaaat caatacgtgg ttttaacggt tcggtcatcg caatttatct
     1081 cctattaaaa attccagcgc ggcatcaaga cgaatatgcg gcagcggttt gtcgacatcc
     1141 atcacctgcg ggcgaaacgc ttcaaactgg aacccttgct tgtcccagaa cgcctgaccg
     1201 ggcaaacgcg caggaacttc accggggtaa acggtgagag gcgcaccatc gctgagccga
     1261 ttaccgcgca atgccggaat tttctcgccg ttgacatcaa taatgccgct ggtggtcgcc
     1321 tgcaccgagg ccagccccag gcaatccata ctaatccctt caaacgccgc attttgccag
     1381 gcatcctgaa tcagttgttg cagtaatgaa accatattgg cgtgctgatc gaccgttaca
     1441 tggtccgctt tcgttgcggc aaacagcagt ttgtcgataa ccggcgagaa taagcggcga
     1501 aagagcgtgc gctgcccgta gtggaaactt tgcatcagct gcgtcagtgc cagacgcata
     1561 tcattaaatg cctgtggccc actgttgaga ggttgcaggc aatccaccag cacaatctgg
     1621 cggtcaaagc gcagaaaatg attcttatag aaccccttca ccaccttctc gcagtaataa
     1681 ttaaaccgct cgcgcagcat tccggcattg gtgtgcttat cggcctgcgc cagtttggac
     1741 tcgccccagg tatcgacatc cggccacggg aagaattgca gcgcgggcgc acctgccata
     1801 tctcctggca agacaaagcg ccctggctga ataaagtgca gcccctgctg tttacagtgg
     1861 tggagataat cggtccacgc ggcggcaatg tctgccagcc ggttttcgtc ggcaggtgct
     1921 agcgggtcca gcccttcgca catcattcgc catttgaccg accattcgcc gcgctgacca
     1981 ttgagtaagc ccgtcatctg gcgtgaccag cttaaatagt cctgcgccag catcggcagg
     2041 tcgagcaacc attcgccggg ataatccaca atctccagat acagcgtgga ggtgtctttg
     2101 aagtggcgta gcagcgaatc gttcgattta aaacgtagcg ccaggcggat ttcactgacg
     2161 ccgcgcgtag gcgtcggcca ggcgggagga tcgccatata actgcgccag tccttcatca
     2221 taggtgaagc gcggaatgcc aaagtcacgc tgaggaatgc gttttacgcc cagcaggcgc
     2281 tcttcacgca ccgcgcttaa cagcggcaag cgtgctccgg cgtgaatatt gagcaactga
     2341 ttgaccatcg cagtgataaa cgctgttttg ccgctgcggc ttagtccggt tacggcgagg
     2401 cgcagatgtc tgtcgacacc ccgattcacc agcgcattaa gttcattttt aagtcgcttc
     2461 attgctgtcc tgtgctgctc tgaagaccag aaaaaaatca ttcgtaagga tatgaatgtg
     2521 ggggccaggc cattgattat caactgcccg tgttgtcatc gacgggcgaa ctccataaga
     2581 tgtgcagcat tcacgttcgg acggagttcc tgacctatgt cccctactat ttatgatatt
     2641 gccagggttg caggcgtatc aaaatccacc gtatcacgcg tgctgaataa gcaaactaat
     2701 atctccccgg aagcgcgcga aaaagtgtta cgggccattg aagaattaca gtatcagcca
     2761 aacaagctag cccgcgcgct gacctcttcg ggttttgatg ccattatggt gatttctacc
     2821 cgttcgacca aaactacggc gggtaatccg tttttctctg aagttttgca tgccatcacc
     2881 gccaaagctg aagaagaagg tttcgacgtg atattgcaga cgtcgcacaa cccggcagaa
     2941 gacttacaaa aatgtgaaag caaaattaag cagaaaatga ttaaaggcat tattatgcta
     3001 agttcgccag cggatgagtc attttttgcc caactcgata aatacgatat tccggtggtg
     3061 gtgattggca aagttgaagg tcaatatgcc catgtttatt ctgtcgatac cgataatttt
     3121 ggcgacagca ttgcgttgac cgatgcatta attgaaagtg ggcatcaaaa tattgcctgc
     3181 ctgcatgctc cgcttgatgt ccatgtttca gtggatcggg taaatggtta taagcagagc
     3241 ctggctgcgc ataatattgc agtgcgtgat gaattgattg ttgacggtgg ttatacccat
     3301 gaaacagcac tgcaagccgc gagagaatta ttaagccagt caccgttgcc tgaggcggtg
     3361 tttgccactg acagtctgaa attaatgagt atttatcgtg cggcagcaga gaaaaatatt
     3421 gctattccgc agcagttagc ggtggtgggt tatagtaatg aaacgctgtc gtttatatta
     3481 acgcctgcac cgggcggcat cgatgttccg acgcaggagt taggtcaaca aagctgcgag
     3541 ttattattcc gcttaatttc tggaaaaccg tcaccacaga atattaccgt tgccacgcat
     3601 atgacgctga aataaatacc agctaccttg cctgaatgcc ggatgcgatg ctagcgcatc
     3661 ttatccggcc tacatttata gatcggataa agtactcgcg ccgcatccgg caatttagcc
     3721 cattatcaga acgagtaatt tacgccgaca cctgcataat ggaatttatc actgtctcct
     3781 tcgtcgtgat cctgccactc aaacgcgtat tccagcgaaa cggaaagtcc gttctggaaa
     3841 tcataaccgt aaaataaacc tacacggtta aaatcatggc cttcacgttc aatatcatcc
     3901 tgccagtccc agttactcca gcgatccagc ccaatgcgcg tatacggcgt caccgagtgg
     3961 ttgccgagcg tcagcggcaa ataggcgcga atttcttgcg tggaaaactc accgttattg
     4021 cggctgtcgt ccatattgaa gccgcgctcg agataatagt tcactcgcaa ggcaaccgtt
     4081 tcgttgaagg tatattgcag acctgtttcc gtttcgacac gggtatcagc gtaaccggta
     4141 gtgttcagat cgttggcaaa tttatacatc gacaaccaac cgttgaaacg taaatcgtca
     4201 gtcagtttca catcccagtc tggcgcgatt ttccagcgct gcatattcgc cgtgtcttta
     4261 cccggttcat caacgtagtg ataaccataa ttacggaaac cgccggtcag gccgaaactg
     4321 aaatcatcgt tttcgaggaa ctgataatgc acctccagct ccgggcgatc aaaccacgtt
     4381 ccacgtttac ccgcgctata atctaccggc ccttcctgat aataggccag agcaattctc
     4441 cacggcccgt tggcggcatt aaaatagact gaaggctccg c
//
LOCUS       DAJTZL010000240         4463 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.241, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000240 DAJTZL010000000
VERSION     DAJTZL010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4463
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.241"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..345
                     /locus_tag="OO544_003880"
     CDS             <1..345
                     /locus_tag="OO544_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail tape measure protein"
                     /protein_id="HCR6769367.1"
                     /translation="DSSASAADKAGAVGSLAGSIAGGALGAAAGPVGVAIGSTVGSYL
                     GDYLGGWLTQAWQKLRGSSDENGGQATAKTAARVELVAPEGWRARSIDVDDTAQHGLD
                     VNVWNGGNYGLY"
     gene            332..1690
                     /locus_tag="OO544_003881"
     CDS             332..1690
                     /locus_tag="OO544_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization protein"
                     /protein_id="HCR6769368.1"
                     /translation="MASTDTPGRGSFRGVPFLVYQEQRERGGRNIVRREYPLRESGGA
                     DDLGPKLPEFTFTVLVTGDDLQTQRSRLRDALRAPGAGELMHPDYGTLNVLINSFESR
                     YNASEQGTVEFTINVIPASDDTAPAVAEDTAAALEQKSGSAMNQLFNTLSDGWTVISD
                     GLHDVQAMTDTISDKIDALENAVSGMGIVQDISAFTASFTALKGNAAALINAPRRMAE
                     SLAGMFAVLTGLPGDPSLSLTGKAGTPSGSLTTNRDSMAEQAMPQLYRTLSSLRYTLS
                     EQDDPQRLIGLTPAAQKNIRLLRSVMQSATLVSQAQTLGKLLDQVIRQNTTHVQDTDR
                     SGGLAWLESSADVQRINRDLSDAMEQQVLDLSAQGYTGTALALRDAGLALTEDLTTRS
                     VHLPGAFQVMVRTTEPALVTLYRATGNSSRWQYFVRRNNIPDPVFVPGGRSVEVISEQ
                     QD"
     gene            1674..2798
                     /locus_tag="OO544_003882"
     CDS             1674..2798
                     /locus_tag="OO544_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6769369.1"
                     /translation="MSSRIELYIGGSIFSGWLTVSVRRSLEHLAGSFELGLMLPGERI
                     PSALRTGQSLTLRINGQTVISGWLDQVSQRISATRHQISISGRDKTGDLVDCAAIHPG
                     SQWRNRTLAQIAADLCAPFGIAVRWQVNDDTAARPFSSFTLENSETVADALTRAARHR
                     GVLVTSNADGDLVFTQAGSQQTDRLVLGDNLLDADYNTDWRGRYSEYRVRGHGRGGGK
                     RGDSESAARLAAPVGVISDEQIGRYRSKIILADQQTDTTGARQRALREMRRAIARSER
                     FSATVRGWFRDDGRLWDVNLLTGVSALRFGIEQTELLVCQVEFLLDEHNGEVTRLVLA
                     PRDGFIVPAEPDSKGRGGSGDDVDAFIRQQMKKQGISFDE"
     gene            2788..3402
                     /locus_tag="OO544_003883"
     CDS             2788..3402
                     /locus_tag="OO544_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HCR6769370.1"
                     /translation="MMNDEVFSRLIAPVTRGIRLLFGRGVLTGTHDELKMQNVQLTGM
                     DGETFDDVERPQQYGQISVPLPGAETFFACLGGQRDQTVVLVVEDRRSRPTGLTAGDT
                     GVYHHEGHRIRLTKDGRIIVTCKMLEIYADEGMRVDTPEATFTGNVTVDKNLHVKGNF
                     VLDGTGKSDGLFTMSDAVIAGITYSGHVHQDNGKGSKTGAPENG"
     gene            3395..3832
                     /locus_tag="OO544_003884"
     CDS             3395..3832
                     /locus_tag="OO544_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HCR6769371.1"
                     /translation="MADIAIVWDQGCGSLQLNGADLLTDDSLLTAVLISLFTDRRALA
                     SDEIPDGTRDRRGWWGDSFRPRPIGSRLWLLSREKTLASVISRARAYADEALGWLSQD
                     GVASSVVCHAERVGHARLALSVRITLPDGSVRPMIFYADLKGE"
     gene            3832..>4463
                     /locus_tag="OO544_003885"
     CDS             3832..>4463
                     /locus_tag="OO544_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313014.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HCR6769372.1"
                     /translation="MPYQPLPLAQLITQTQQDISQRLPGSQPGVNETTLNAIAYAQAG
                     LSAQEHEHLAWIARQIIPTEADEAELLKHCAFWGVMRKPASRGDGPVQLMLTTDAGIK
                     EGVLLQRSDGVVYRITTSLTGKAGTLNVSVEAESAGRAGNAPAGTKLTFITPQAGINQ
                     TATVTGTGITGGADVETVPELLSRLVFRVQNPPSGGTQYDFERWAREVPGV"
BASE COUNT          908 a         1201 c         1447 g          907 t
ORIGIN      
        1 gacagcagcg ccagtgcagc cgataaagcc ggtgccgtgg gttctcttgc cggaagtatc
       61 gccggtggcg cgctgggggc ggctgccggt ccggtcgggg ttgccattgg ttccacggtg
      121 ggcagctatc tcggtgatta tctggggggc tggctgacgc aggcctggca gaaactgcgt
      181 ggcagcagtg acgaaaacgg cggacaggcc accgcgaaaa ccgccgcacg ggtggagctt
      241 gtggcccctg aaggctggcg ggcgcgcagt attgatgtgg atgataccgc acagcatggc
      301 ctggatgtga acgtctggaa cggagggaac tatggcctct actgacacgc cggggcgtgg
      361 ctcctttcgt ggcgtaccgt tcctggttta tcaggagcag cgggaacggg gcggacgtaa
      421 cattgtgcgc cgtgaatacc cactgcgtga aagcggcggt gcggatgatc tggggccaaa
      481 actcccggag ttcacattca cggttctggt gaccggagac gatcttcaga cacagcgaag
      541 ccgcctgcgt gatgcgctgc gtgcacccgg tgccggtgaa ctgatgcacc cggattacgg
      601 cacgttaaac gtgctgataa acagctttga aagccgttat aacgccagcg agcagggaac
      661 ggttgagttc acgatcaacg tgatccccgc cagtgacgat accgcccctg cggtggcaga
      721 agatacggca gctgcgctgg agcagaaaag cggctcagca atgaatcagc tgtttaacac
      781 gctttcagac ggctggacgg tgatttcaga cggtctgcat gatgtacagg ccatgaccga
      841 caccatcagc gataagattg atgcactgga gaatgccgtt tcggggatgg ggattgtgca
      901 ggatatcagt gcctttacgg ccagttttac tgccctgaaa ggcaatgccg ccgccctgat
      961 taatgcgccc cgtcgcatgg ctgaatcgct ggccggaatg tttgccgtgc tgaccgggct
     1021 gcccggcgat ccttccctgt ctctgaccgg aaaagccgga acgccgtccg gcagcctgac
     1081 cacaaaccgt gacagcatgg cagagcaggc catgccgcag ctgtaccgca cactttcctc
     1141 cctgcgttac accctcagcg aacaggatga tccacagcgc ctgattggcc tgacgcctgc
     1201 ggcacagaaa aatatccgtc tgctgcggag cgtgatgcag agtgcaaccc tggtgtcgca
     1261 ggcacagacg ctcggaaaac tgctggatca ggtcatccgg cagaatacga cgcacgttca
     1321 ggatactgac cgttcgggcg gactggcctg gctggaaagt tctgctgatg tgcagcgtat
     1381 caaccgggat ttgagcgatg ccatggaaca acaggtgctg gatctgtccg cacagggcta
     1441 taccgggact gcccttgcat tacgtgatgc agggcttgcg ctgacagaag atttaaccac
     1501 acggagcgtg catcttcccg gtgcttttca ggtcatggtt cgcaccaccg aaccggcact
     1561 ggtcacgctg taccgtgcca ccggcaacag cagtcgctgg cagtattttg tccgcagaaa
     1621 caatatcccc gatccggttt ttgttccggg tggccgcagc gtggaggtga tcagtgagca
     1681 gcaggattga actgtatatc ggtggcagta ttttttccgg ctggctgacg gtcagcgtcc
     1741 gtcgctcgct ggagcatctg gccgggtcgt ttgaactggg gctgatgctg cccggtgagc
     1801 gcatcccctc agccctgcga accggtcagt ccctgacgct cagaattaac gggcagacgg
     1861 tcatcagtgg ctggctggat caggtcagtc agcggatcag tgcaacccgt catcagatca
     1921 gcatcagcgg tcgggataaa accggcgacc tggtggactg tgccgccatc catcccggca
     1981 gccagtggcg caaccggacg ctggcacaga ttgccgcaga tttatgtgcg cccttcggga
     2041 tagccgtgcg ctggcaggtt aacgacgaca cggctgcacg ccccttcagc tcttttacgc
     2101 tggaaaactc agaaaccgtg gcggatgcgc tgacccgtgc tgcccggcat cgcggggtgc
     2161 tggtgaccag taatgctgac ggcgatctgg tgttcaccca ggcaggaagc cagcagacgg
     2221 acagactggt gctgggagat aacctgcttg atgccgatta caacacggac tggcgaggac
     2281 gatacagcga ataccgtgtc cggggacacg ggcgcggtgg tggcaaacgg ggagacagcg
     2341 aatccgccgc ccgtctggca gcacctgtgg gcgtcatcag tgatgagcag atcggccgct
     2401 accggtcgaa aatcatcctc gccgatcaac agacagacac caccggtgca cggcagcgtg
     2461 ccctgcgtga aatgcgccgt gcgattgccc gttcagaacg gttttctgcc accgtgcgtg
     2521 gctggttccg ggatgatggc cggttatggg atgtcaatct gctgaccggt gtttcagccc
     2581 tgcgtttcgg tatagaacag actgaactgc tggtctgtca ggtggagttt ttacttgatg
     2641 aacataacgg ggaagtcacc cggctggtac tggcaccgcg tgacggcttt atcgttccgg
     2701 cagagccgga cagtaagggc cggggtggtt ccggtgacga tgttgatgcc tttattcgcc
     2761 agcagatgaa aaaacagggg atcagctttg atgaatgatg aagtgttcag ccgcctgatt
     2821 gccccggtaa cgcgcggtat tcgcctgttg tttggccggg gcgttctgac cggcacacat
     2881 gacgaactga aaatgcagaa tgtgcagctc accggcatgg acggcgaaac ctttgatgat
     2941 gtggagcgtc cccagcagta cgggcagatc agcgttcccc tgccgggtgc ggaaaccttt
     3001 tttgcctgtc tgggcggaca gcgggatcag actgtggtgc ttgtggtgga agaccggcgc
     3061 agccgcccga ccggactcac agccggagat acgggggtgt atcaccatga ggggcaccgg
     3121 atacggttaa caaaggatgg tcgcattatt gtgacatgca aaatgctgga gatttacgcc
     3181 gatgagggga tgcgggtgga tacgccggag gccaccttta cgggcaatgt gacggtggat
     3241 aaaaacctgc atgttaaggg taatttcgtg cttgatggta caggtaaatc agacgggctg
     3301 ttcacgatgt ctgatgccgt tattgccggg atcacctatt ccggccatgt gcatcaggat
     3361 aacggcaaag gcagtaagac aggagcgccg gagaatggct gatattgcaa ttgtatggga
     3421 tcaggggtgc ggttcgctgc aactgaacgg tgccgacttg ctgacggatg acagtctgct
     3481 gacggccgtt ctgatttcgc tgtttactga ccgccgggcg ctggcatcgg atgaaatccc
     3541 tgacggtacg cgcgaccgcc ggggatggtg gggagacagt tttcgcccgc gccccattgg
     3601 ctcccgtctg tggcttctca gccgggaaaa aacgctggcc tcggtgataa gccgtgcccg
     3661 tgcttacgcg gatgaggcgc tgggctggct cagtcaggat ggcgtggcgt catccgtggt
     3721 ctgtcatgca gaacgtgtgg gacacgcgcg gctggcgctt tcggtgcgca taaccctgcc
     3781 tgatggttca gtgaggccca tgatttttta tgctgatctt aagggggagt aatgccttat
     3841 cagcctttac cgctggcaca gttaatcacg cagacacagc aggatatcag ccagcgcctg
     3901 cccggctcgc agccgggtgt gaatgaaacc accctgaatg ccattgctta cgctcaggca
     3961 gggttatctg ctcaggagca tgagcatctg gcctggattg cgcgtcagat catcccgacc
     4021 gaagccgatg aagccgaact gctgaaacac tgtgcattct ggggcgtcat gcgtaagcct
     4081 gcctctcggg gggacgggcc ggttcagctg atgctgacca cggatgcggg gatcaaggaa
     4141 ggtgtgcttc tccagcgaag tgatggcgtg gtttaccgta tcaccacctc cctgaccggt
     4201 aaggccggta cgctgaatgt cagtgtggaa gccgaaagtg ccggtcgtgc ggggaatgcc
     4261 ccggcaggga caaaactgac atttatcaca ccgcaggcgg ggatcaacca gacggccacg
     4321 gtgaccggaa cggggatcac cggtggtgcg gatgtggaaa ccgtgccgga gctgctttcc
     4381 cgtctggttt tccgggtgca gaatccaccg tccggcggca cgcagtatga ttttgaacgc
     4441 tgggcgcgtg aagtgccggg cgt
//
LOCUS       DAJTZL010000241         4438 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.243, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000241 DAJTZL010000000
VERSION     DAJTZL010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4438
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.243"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            52..936
                     /locus_tag="OO544_003886"
     CDS             52..936
                     /locus_tag="OO544_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HCR6769373.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHNEDADGVALTFELTQSEETKKFCPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSISMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(987..1841)
                     /gene="yeaE"
                     /locus_tag="OO544_003887"
     CDS             complement(987..1841)
                     /gene="yeaE"
                     /locus_tag="OO544_003887"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HCR6769374.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLRDNVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHRSGSFAFEETVAAMEKLIAQGKIRRWGVSNLDYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISSAQVLLAWVISHQGVMAIPKAATIAHVQQNAAVLEVELSSAELTMLDKAYPAPK
                     GKTALDMV"
     gene            complement(1931..2677)
                     /gene="mipA"
                     /locus_tag="OO544_003888"
     CDS             complement(1931..2677)
                     /gene="mipA"
                     /locus_tag="OO544_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HCR6769375.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLTGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            3113..>4438
                     /gene="yeaG"
                     /locus_tag="OO544_003889"
     CDS             3113..>4438
                     /gene="yeaG"
                     /locus_tag="OO544_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HCR6769376.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMKGLSTRFAFKILSRVFNFDHVEVAAN"
BASE COUNT         1118 a         1113 c         1079 g         1128 t
ORIGIN      
        1 cttcggtcgc tctttttttt acctgataaa atgaagttaa aggactgcgt catgattaag
       61 aaaatttttg cccttccggt catcgaacaa atctcccctg tcctctcccg tcgtaaactg
      121 gatgaactgg acctcattgt ggtcgatcat ccccaagtaa aagcctcttt tgcattacag
      181 ggcgcacacc ttctctcgtg gaaacctgcg ggtgaagaag aagttctgtg gttgagcaac
      241 aacacaccgt tcaaaaatgg cgtcgctatt cgcggtggcg taccggtttg ctggccgtgg
      301 tttggtccgg cggcacaaca aggtctgcct gcgcacggtt ttgcccgcaa cctgccgtgg
      361 acgctgaaat cacataatga agatgctgat ggcgtagcgc tgacttttga attgacgcaa
      421 agcgaagaga cgaaaaaatt ctgtccgcac gactttacgc tgttagcgca tttccgcgtg
      481 ggtaaaacct gtgaaatcga tcttgaatca catggcgaat ttgaaaccac ctctgccctg
      541 catacctact ttaacgtggg tgatatcgct aaggtaagcg tcagtgggct gggcgatcgc
      601 ttcattgata aagtgaatga cgcgaaagaa gatgtactga ccgacggtat tcagaccttc
      661 cctgaccgta ccgatcgcgt gtatctgaat ccgcaagatt gcagcgtgat taatgatgaa
      721 gcgctgaatc gtattatcgc cgtaggccac cagcatcatc tgaacgttgt aggctggaac
      781 ccgggaccgg cgctttcaat tagcatgggc gatatgccgg atgatggcta caaaacattt
      841 gtttgtgtag aaacggctta cgcttcagaa acgcaaaaag tcaccaaaga gaaacctgca
      901 catctggcgc aatccattcg cgttgcgaaa cgttaatttg cgttaatgtt gtgtgccggg
      961 tgcgatgcat ccggcacgta tcaacatcac accatatcca gcgcagtttt tccttttggt
     1021 gccggatacg ctttatccag catggttaat tccgctgacg aaagttcgac ctcaagcaca
     1081 gccgcattct gttgaacatg ggcaatcgtg gccgcttttg gaatcgccat cacaccctga
     1141 tggctgatta cccacgccaa caatacttgt gccgagctga tattgtgagc atgtgcaatt
     1201 tcgttgacta ccgcgttttt taacagtcca ttgcgcaacc gcccggcctg ggctaatgga
     1261 ctgtaagcca tcaccggcat ctgctgttgc tggcaccagg ggagtagatc gtactcaatc
     1321 cctcgtgaac cgagatggta aagcacctga ttagtggcac actgatttcc ccccggcagc
     1381 tgccagagtt cctgcatatc agcataatca aggttagaaa cgccccagcg gcggattttt
     1441 ccctgggcga tcaatttttc catcgctgca acagtctctt caaaagcgaa actgccagac
     1501 ctgtgtaata agtagagatc gagataatca gtattgagac ggcgtaaact ggcttcgcat
     1561 gcatttatcg ctttttgccc gccagcattc cacggataga ctttagagac gagaaagacg
     1621 ttatcacgca gaccggttaa tgcttcccca accacctttt cggcaccgcc atcggcatac
     1681 atttcggcgg tatcaatgag ggttaaaccg agctcaatgc ccgcgcgtag tgcagcaact
     1741 tctgttttgc gctgactggc atcttcgccc atataccatg ttccctgccc tatggctggc
     1801 agtgagacat cgccactaaa ttgaatcatt ttttgttgca ttgtttcctc ccaggtattg
     1861 caccaccgta atgcaaaaca gggcgtgacg ccctgttttt atgcacaaaa tgccctggaa
     1921 agatgcatta tcagaatttg taggtgatcc cggtagaaat caggccagtc caggatttat
     1981 ccaccatcgg gctgtcagta acttcatcag acagacgggt atagcgcgcg gtaccgtaaa
     2041 cactccagtc gccgaggaag ttgtagctgg cgctcagctc caggtaaggg ctccagctgt
     2101 cgttcgggtt atagccacgc agaccgctgc gagcggactc tttgcgcgat acgccataat
     2161 agtattcgtt ctggttttcg ctgttccact gcacaccgat acccggggtc actgtcaggc
     2221 cgccgttggt gtaacgatac aaccaggcca tatcccagac aatgccgttg ctgttatcca
     2281 gggtatcgcc agtcagggtg gtacgcaggt aaccgtactg ggtaaagtga gcataagaca
     2341 gaccagccat catggtgctc ttacggtcat ccaggtgacg catttggtga tcgccactgt
     2401 ctttcgcttt gaagtaaagc ggcgaccaat aagcggtaat tgaaagtttg tccgttgcgt
     2461 cattccacag gtagtaacca ccacctaagc cacggaacca gaagttatcg ccttcatagt
     2521 tgattaccgg tactgggtaa acatcggtat cgtaatcttt atatgggtgt tcaacgacac
     2581 cgacgcctgc gcccagggaa aatttacctt cagcgtgcgc tacgcctgca gacgttgcga
     2641 taagcactcc aagtgccaga agtttgagtt tggtcacaat taatcattcc ttaaacaaat
     2701 gtttagcggg cgacaaagtt tacccgtcaa tacatcgaaa cccaaccttt ttacgttttc
     2761 attttttaaa gtaactgttt aattttcctg acgcggatga caccgcgctt acagccaaat
     2821 gaatttagcg ttactggcga gccttgtctt tacattaatt atgcaaaatt tatggatgag
     2881 ttgttgatat gccattgaaa ttaagaaagc cgtgcaggca agttttccat ttgccatcta
     2941 cgcttaattt tgaaggtgta tcaccgggca cgttgttctc atcgtcgata aaatggcatg
     3001 agagttgctg tgttttagca agagacgtcg ttcagtttac ctcttccggg agcctctact
     3061 attcatatga acggctctta acctgtgcta aaaaacgaaa ggacggcata ccatgaatat
     3121 attcgatcac tatcgccagc gatatgaagc tgccaaggac gaagagttca cactgcagga
     3181 gtttcttacc acttgtcggc aagatcgcag tgcttatgcc aacgcggctg agcggctatt
     3241 gatggctatc ggtgagcctg tcatggtcga tacagcccag gaacccagac tttctcgact
     3301 cttttctaac cgggtcattg cacgttatcc ggcatttgaa gagttttacg gcatggaaga
     3361 cgcgattgaa cagattgtct cttatctgaa acacgcggct caggggctgg aagagaagaa
     3421 acaaatcctg tatctgctgg ggcctgtggg tgggggtaaa tcatcgcttg ctgagcgact
     3481 gaaatcatta atgcagctcg taccgattta tgtattgagc gccaacggtg agcgtagccc
     3541 ggtcaacgat catccgttct gtcttttcaa tccgcaggaa gatgcgcaga ttctggaaaa
     3601 agagtatggc attcctcgcc gctatctcgg caccatcatg tcgccgtggg cggcaaaacg
     3661 cctgcatgaa tttggtggcg atatcactaa gttccgggta gtgaaggtct ggccgtcaat
     3721 tctgcaacaa attgctatcg ccaaaacaga acccggtgat gagaacaacc aggacatctc
     3781 cgcgctggta gggaaagtcg atattcgtaa actcgaacac tacgcgcaga atgacccgga
     3841 cgcctacggc tattccggtg cgctgtgccg cgccaatcag gggatcatgg aatttgtcga
     3901 gatgtttaaa gcaccgatta aagtgctgca tccgctgtta accgctactc aggaaggtaa
     3961 ctacaacggg acggaaggta tctccgccct gccgttcaac gggattattc tcgcccactc
     4021 gaacgaatcc gaatgggtca ctttccgtaa taacaaaaac aacgaagcct tcctcgaccg
     4081 tgtttacatc gtgaaggtgc cgtattgctt acgcatttcc gaagagatca aaatctacga
     4141 gaaattgctt aatcacagtg aattgactca cgccccatgc gcccctggca cgctggaaac
     4201 actgtcacgt ttttccattc tttcgcgcct gaaagagcca gaaaactcca gcatttattc
     4261 aaagatgcgg gtttatgatg gcgaaagtct gaaagacacc gatcccaaag ccaagtcgta
     4321 tcaggaatat cgtgactacg ccggtgtcga tgaagggatg aaaggtctgt cgacgcgttt
     4381 tgcgtttaag atcctctccc gcgtgttcaa cttcgatcat gtagaagtgg cggcaaac
//
LOCUS       DAJTZL010000242         4411 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.244, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000242 DAJTZL010000000
VERSION     DAJTZL010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4411
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.244"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(173..2122)
                     /locus_tag="OO544_003890"
     CDS             complement(173..2122)
                     /locus_tag="OO544_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR6769377.1"
                     /translation="MADSFQLKAIITAVDKVSAPLKGMQRQLKGFKKEFASLLLGAAG
                     AGTAVLGALALPVKSAIALESKMADVRKVVDGLDTPEAFKAMTEQVRDLSTELPMSAE
                     GIAEIVAAGGQAGIARDELMQFTDDAVKMGVAFDTTAEESGQMMAQWRTAFKLTQGEV
                     AGLADKINYLGNTGPASAKKISDVVTRIGPLGSVAGVASGEIAAMGATIAGMGVESEI
                     AATGIKNFMLSLTARDSATKSQKKVLRSLRISPKKLAADMQKDARGAMLHVLDSLAKV
                     PKEKQAAVLKALFGKESLGAIAPLLTNLDLLRTNFNRVADAQQYGGSMQKEYAARAAT
                     TENQLLLLQNQINAISSTLGETFLPSLNEGIKEMKPFLEEVRTFVRENPEVVKTIAKT
                     GAALLTMGVAIGTLTRITKIMGSVMNMTPAKGLIALLVGGAYLIIDNWETVGPVVKKV
                     WQEVDQVVRAMGGWEQAVKTIATVSALYIGVKAVASIRAATVAQNQWTTAAGKTGLKL
                     KGLGKISLIGGLLELGMMAQEFEKAHPWLVKNFVADALNSGFGLNDKFDEWGKQFHDF
                     VYDMTGWQMPRGDGYLSPDKRYTPNVSLERNQLLSLASSPATRSELKVTFDNAPPGMR
                     VIDLPKTGDPFMKITHDVGYSPFKR"
     gene            complement(2207..2542)
                     /locus_tag="OO544_003891"
     CDS             complement(2207..2542)
                     /locus_tag="OO544_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HCR6769378.1"
                     /translation="MAMNVTEIVLKKPVTAHNEMLHVLELREPTYDEIEALGFPFIIS
                     GEGSIKLDSQVALKYIPLLAGIPRSSAAQMAKLDIFKTSMQILRFFTQSETGSTSGND
                     STMLPGSGN"
     gene            complement(2542..2898)
                     /locus_tag="OO544_003892"
     CDS             complement(2542..2898)
                     /locus_tag="OO544_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HCR6769379.1"
                     /translation="MAKIAGTCFFKVDGQQLSLTGGIEVPMNTNVRDDIVGMAGDVDY
                     KETWRSPYVKGTFKVPKNFPVDKITTSDQMTITAELANGMVYVLSAAWLHGEANHNAE
                     EGTADLEFHGEEGGYQ"
     gene            complement(2898..4394)
                     /locus_tag="OO544_003893"
     CDS             complement(2898..4394)
                     /locus_tag="OO544_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HCR6769380.1"
                     /translation="MTISFNTVPSNTLVPLFYAEMNNSAANTAVTSAPALLIGHASND
                     AAIEVNSLVLMPSADYARQICGAGSQLARMVEVYRQTDPFGELYVIAVPEARGAAATV
                     RVTVAGEAEESGTLSLYVGRSRVQVPVVNGDDATAVATAIKEAVNGVITLPFTASSDA
                     GVVTLTARHKGLYGNELPVCLNYYGSGGGEILPAGLRVVTEVGAAGSGAPDLTAAVAA
                     MGDEAFDFIGLPFNDAASINMMMTEMNDSSGRWSYARQLYGHGYTAKLGTLSELVGAG
                     DMHNQQHITLAGYEKETQSPVDELVASRLAREAVFIRNDPARPTQTGELVGMLPAPKG
                     KRFIMTEQQTLLSHGVATAYVEGGTLQIQRSVTTYKKNAYGVADNSYLDSETLHTSAY
                     VLRKLKSVITSKYGRHKLANDGTRFGPGQAIVTPAVIKGELLATYRQMERAGIVENYD
                     LFKQYLIVERDADNPNRLNTLFPPDYVNQLRVFAVVNQFRLQYSEESA"
BASE COUNT         1020 a         1315 c          970 g         1106 t
ORIGIN      
        1 aacgttacta ataaatcgtg aaacaatctc ctcatttgtt agtattttcg cctgagcaca
       61 gcaagaggtt aatattgcaa ccgtcattaa aaatggcttt atcacaaaac aaactccatt
      121 gttagcatgt tacgcgataa ttttgcctca tctttcacca caaaaaaaga actcaccttt
      181 taaatggaga atacccaaca tcatgggtga ttttcataaa gggatcgcct gttttcggca
      241 aatcgataac gcgcattcca ggcggcgcgt tgtcaaacgt caccttaagt tcgctgcggg
      301 ttgccggtga agatgcaagg cttaacagct gattcctttc cagtgataca ttcggggtgt
      361 agcgtttatc gggagataaa tacccatcac cacgtggcat ctgccatccg gtcatgtcat
      421 agacaaaatc atggaactgt ttgccccact cgtcgaactt atcattcaga ccaaatccac
      481 tgttcagtgc atcagccaca aaatttttaa caagccaggg atgtgctttt tcaaactcct
      541 gcgccatcat gcccagttca agcaatccac caatcagact gatctttccg aggcccttta
      601 atttcagccc tgtttttcct gccgccgttg tccattgatt ctgggcgact gttgccgcac
      661 gaatacttgc caccgccttc acaccaatat acagggcaga aaccgttgct attgttttta
      721 ctgcctgttc ccatcccccc attgctctga caacctgatc aacctcctgc cagacttttt
      781 tcacaaccgg accgaccgtt tcccagttat caattatgag atatgcacca ccgacgagaa
      841 gcgcaattaa tccctttgcc ggcgtcatat tcatcacgct acccatgatt tttgttatgc
      901 gtgtcaatgt gccaatcgcc acgcccatcg tcagtaatgc cgcaccagtt ttcgcgatgg
      961 tttttacgac ctcaggattt tctctgacaa acgtgcgcac ttcttccaga aaaggcttca
     1021 tctcttttat gccttcattg agtgaaggca ggaaggtttc ccccagcgtg gaagaaatcg
     1081 cattgatctg gttctgcagc agcaacaact gattttctgt cgtcgcggca cgggcggcat
     1141 attctttctg cattgagccg ccatactgct gcgcatccgc cacacgatta aagttggttc
     1201 gcaacaaatc cagattcgtg agcagcggcg caatggcccc cagggattcc ttcccgaaca
     1261 gcgctttcag cacagcagcc tgtttttctt tcggcacttt tgccagagaa tccagtacgt
     1321 gcagcatggc cccgcgggcg tctttctgca tatccgccgc cagttttttc gggctaatgc
     1381 gcaacgagcg gagcactttt ttctgcgatt tggtggcaga atccctcgct gtcagcgaca
     1441 acataaaatt ctttatcccc gtcgcagcaa tttctgactc cacccccatt ccggcaatgg
     1501 ttgcccccat tgcggcaatc tctccggagg ccacacccgc aacactgcct aaagggccaa
     1561 tacgggtcac aacatcagaa atctttttcg cacttgcagg accggtatta ccaagatagt
     1621 taatcttatc cgcaagccct gccacctctc cctgtgtcag tttaaaggca gtgcgccact
     1681 gcgccatcat ctgaccggat tcttctgccg tggtgtcaaa ggccacgccc atcttcacgg
     1741 catcgtcagt aaactgcatc agctcgtcac gggcgatacc agcctgacca cccgccgcca
     1801 caatttcggc gataccttcc gccgacatgg gcagttctgt tgacaggtcg cgcacctgct
     1861 ccgtcattgc cttaaacgct tccggcgtat ccagaccatc caccactttc cggacatccg
     1921 ccatttttga ttcaagggca atggcagatt tgaccgggag cgccagcgcc cccagtactg
     1981 cggttccggc acccgctgcg cccagtaaca ggctggcaaa ctccttttta aaacctttca
     2041 gctggcgctg cattcccttc agcggtgccg ataccttatc cacggcagtg atgatcgcct
     2101 tcagctgaaa actgtcagcc atgcttcatc tcctcgttaa tgcggacggc ctcggcttcc
     2161 agttctgtaa actgcgaaac agccccccgt cgcagctcca gtggattcag tttccagaac
     2221 caggcaacat tgtagagtcg tttccggagg tgcttcccgt ctccgactgg gtaaaaaaac
     2281 gcaggatctg catgctggtc ttaaaaatat ccagttttgc catctgcgcc gccgatgaac
     2341 gcgggatccc cgccagcaac gggatatatt tcagtgccac ctggctgtcc agtttaatac
     2401 tgccttcacc ggaaataatg aaaggaaaac ccagcgcctc gatttcgtca tacgtgggct
     2461 cgcgcaactc cagcacatgc agcatttcgt tatgcgctgt caccggtttt tttaaaacta
     2521 tttctgtaac gttcattgcc attactgata ccctccctct tcgccgtgga attcaagatc
     2581 tgccgtgcct tcttcggcat tatgattagc ctctccgtgc agccatgccg ccgaaagcac
     2641 atacaccatg ccgtttgcca gctcagcggt aatcgtcatc tggtctgagg tggtaatttt
     2701 gtcgaccgga aagtttttgg gcactttaaa cgtgccctta acgtaaggtg accgccaggt
     2761 ctccttgtaa tccacatccc ctgccatgcc gacgatgtca tcccggacat tggtgttcat
     2821 cggcacttca atgccacctg tcagcgataa ctgctgacca tccactttaa aaaaacaggt
     2881 tccggcgatc tttgccatta tgctgactcc tctgaatact gaagacggaa ctggttaacc
     2941 accgcaaaga cacgtaactg attaacataa tccggcggga acagcgtatt cagtcggttc
     3001 gggttatctg catcacgctc aactatcaga tactgtttaa acagatcgta attttccaca
     3061 ataccggcac gctccatctg acgatatgtc gccagaagtt cccctttgat aacggcagga
     3121 gtgacaatcg cctgccccgg accaaaacgg gtaccatcat ttgccagctt gtgacgtccg
     3181 tacttgcttg tgatgaccga tttcagtttg cgaagaacat atgcactggt atgcagagtt
     3241 tcactgtcca gatagctgtt gtctgccacg ccatacgcat tctttttgta ggtggttacg
     3301 gaacgctgga tctgcaacgt gccgccttcc acataagccg tcgccacgcc gtgagataaa
     3361 agggtctgct gctctgtcat gatgaatcgc ttacctttcg gtgccggaag catccccacc
     3421 agctcccccg tctgtgtcgg acgggcagga tcattccgga taaataccgc ttcacgggca
     3481 aggcgactgg caaccagttc atcgacaggc gactgggttt ctttttcgta accggcaagc
     3541 gtgatatgtt gctgattatg catatctccg gcaccaacca gctctgacag cgttcccagt
     3601 tttgcggtat agccatgccc gtataactgg cgcgcataac tccagcgacc gctgctgtca
     3661 ttcatttcgg tcatcatcat attgatggag gcggcatcgt tgaacggcag accgataaag
     3721 tcgaatgcct catcgcccat agcagcaaca gcggcggtaa gatcaggcgc tccgctaccg
     3781 gcagcaccga cttccgtcac cacccgaagc cctgctggca gaatctcacc accaccagaa
     3841 ccataataat tcagacagac cggtaactca ttaccataca gccccttatg acgggcagtc
     3901 agcgtcacca cacctgcatc agatgacgcc gtaaacggca gggtgataac cccgtttacc
     3961 gcttccttaa tcgcggtggc aaccgcagtg gcatcatcgc cattcaccac aggcacctgt
     4021 acacgggagc gcccgacata cagactcagg gtgccgcttt cctctgcttc tccggcaacc
     4081 gtcaccctga ccgtcgccgc cgcccctctg gcttccggta ctgcaataac atacagttca
     4141 ccgaaaggat ctgtctgacg gtagacttcc accatacgcg ccagctggct ccccgcccca
     4201 caaatctggc gggcataatc tgccgatggc atcagcacca ggctgttaac ctcaatggct
     4261 gcatcgttgc tggcatgccc gatcagtaat gcaggcgcgc tggtcaccgc tgtatttgcc
     4321 gcagaattgt tcatctcggc gtagaacaac ggcaccagcg tattcgacgg aacagtatta
     4381 aaacttatcg tcatgatttt ttagccttat c
//
LOCUS       DAJTZL010000243         4404 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.245, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000243 DAJTZL010000000
VERSION     DAJTZL010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4404
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.245"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(139..3654)
                     /locus_tag="OO544_003894"
     CDS             complement(139..3654)
                     /locus_tag="OO544_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR6769381.1"
                     /translation="MDKNALGFASYWRNSLADAESGKGSFERKDAKNFTHWHGIAAGR
                     LDEAIVSKFFKGEKDDVETVDVILRPKVYFRLLQHGKDRSAGAPDIVTPIVTPALLSR
                     EGFLYPTPATSIPRDLLEPLPKGAFSIGEIGQYDKYKTTHTTFSINFDDSVDKTAETD
                     EEREARYAALQQEWRQYLYDSERLLKSVAGDWIEKPEQYELAEHGYIVKTAQSGGASS
                     HILSLYDHLIVCNKDVPLFNRFASREVHAAESLLAPGAKFSDRLGHSGDKFPLAKAQR
                     DALSHFLDARHGDILAVNGPPGTGKTTLVLSIIATQWARAALEKSEPPVIIATSTNNQ
                     AVTNIIEAFGKDFSQGSGAMAGRWLPELKSFGAYFPSSSRKAEAAKKYQTEDFFNQVE
                     SKEYVEDALLFYLEKAKAAFPGKECSSPEKVIELLHGQLAAKSEQLIRLNATWQTLSQ
                     IRAARELIANDIEQYLDNLNKLLSGQEQKVTLLKSAKTEWKKYRAGESLIYSLFSWLP
                     AVRNKRQYQIQLFLEDKLGALIAGNQWSDPETIERNIDGLLNSAEREQTTYRQQIDSA
                     HEIVLKEQQAVQEWQRLAFDLGYEGDEELSFSQADELADTQIRFPAFLLTTHYWEGRW
                     LMDMASIDDLQDEKKKKGAKGVTARWQRRMKLTPCVVMTCYMLPGNMQISEHKGQRKF
                     EKSYLYDFADLLIVDEAGQVLPEVAAASFALAKKALVIGDTEQIPPIWSIAPAIDVGN
                     MLAEKILSGSTQEEITEKYTAIADLGKSAASGSVMKIAQFASRYQYDPELARGMYLYE
                     HRRCYDNIIGYCNTLCYHGKLLPKRGREESNLMPAMGYLHIDGKGELASSGSRYNLLE
                     AETIAVWLAENQQNIEAHYGKSLHEVVGIVTPFSAQVSTIKQVLGKQGISTGTNEKSL
                     TVGTVHSLQGAERAIVIFSPVYSKHEDGGFIDSDNSMLNVAVSRAKDSFLVFGDMDLF
                     EVQPASSPRGLLAKYLFESEKNALSFDYKERKDLKTAGTKIYTLHGVEQHDNFLNQTF
                     ENTSKHITIISPWLTWQRLEQTGFLDSMIAACSRGINVTIVTDRSYNTEHNDFEKRKE
                     KQQNFKAALEKLNALGIATKLVNRVHSKIVIGDDGLLCVGSFNWFSATREARYERYDT
                     SMVYCGDNLKGEIEAIYNSLERRQV"
     gene            complement(4106..>4404)
                     /locus_tag="OO544_003895"
     CDS             complement(4106..>4404)
                     /locus_tag="OO544_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR6769382.1"
                     /translation="VNKALRVMGYDTTQDVCGHGFRAMACSALIESGLWSRDAVERQM
                     SHQERNGVRAAYIHKAEHLEERRLMLQWWADFLDANRDKGISPFEYAKINNPLK"
BASE COUNT         1141 a         1128 c          905 g         1230 t
ORIGIN      
        1 gagcctctga ttcttccgga cagaagataa tcatgtcggt atgaatgtta taattaagga
       61 ctgtgcaata atagccattc cattcagcca tcttacgctc gaaaacgatc ggtaagatgg
      121 ctactcacca ttcctgctct aaacctgacg cctctcaaga ctattataaa tagcctcaat
      181 ctcacccttc aggttatcac cgcaataaac cattgatgta tcgtatcgtt catatcgcgc
      241 ttcccgtgtc gcactaaacc agttgaacga tcccacacac agcaaaccat catcaccaat
      301 aacaattttg ctatgaacac ggtttaccag ctttgtagca atacccagcg cattcagttt
      361 ctccagcgcc gctttaaagt tctgctgctt ctcttttcgc ttctcaaaat cattatgttc
      421 agtgttgtag cttctgtcag tgactatcgt gacgttaatt ccacgtgaac acgccgcaat
      481 catggaatca agaaaaccgg tttgctccag cctttgccag gtcagccatg gagaaattat
      541 cgtgatgtgt ttactggtat tttcaaatgt ctgattcagg aaattatcat gttgctccac
      601 accatgaagt gtgtagattt tggtcccggc ggtttttaaa tccttacgct ctttataatc
      661 aaaagagagc gcattcttct ctgactcaaa gaggtatttt gccagtaatc cccgtggcga
      721 tgaggctggc tggacctcaa acaggtccat atcgccgaag accagaaaac tgtccttcgc
      781 acgggagact gcaacattca gcatgctgtt atcgctatca ataaacccgc cgtcttcatg
      841 ttttgaatag actggcgaga atatcacaat cgctctttcc gctccctgaa gagagtgcac
      901 ggtgcccact gtgagcgact tttcattcgt gcctgtactg ataccttgtt tgcccagcac
      961 ctgtttgata gtggatacct gagcgctaaa aggcgtcaca ataccgacaa cttcatgaag
     1021 cgatttaccg taatgcgctt caatattttg ctggttctct gccaaccaga ccgctatcgt
     1081 ttcagcctca agcaaattat atcgacttcc actacttgcc agctctcctt taccatcaat
     1141 atggagatac cccattgcgg gcattaaatt gctctcttca cgccctcttt taggcaacaa
     1201 cttaccgtga tagcagagcg tattacagta tccaataata ttgtcgtagc accggcggtg
     1261 ttcatatagg tacataccac gagccagttc gggatcatat tgatagcgcg aagcaaactg
     1321 cgctattttc ataacgctgc cagatgcggc acttttacca aggtctgcga ttgccgtata
     1381 tttctcggta atctcttctt gcgtactgcc agacagaatt ttttccgcca gcatgttacc
     1441 gacatcaatc gcaggagcaa tactccatat tggcgggatc tgctccgtat cgccaatcac
     1501 taatgccttc ttagctaatg caaacgaggc agcagccact tcaggaagca cctgcccggc
     1561 ttcatcgaca atgagtaaat cggcaaaatc atacaaataa cttttctcga atttacgttg
     1621 tcctttgtgc tcacttatct gcatattacc gggcagcata tagcatgtca ttaccacaca
     1681 tggcgtgagt ttcattcgac gttgccaacg ggcggttacc cctttagcac cttttttctt
     1741 cttctcgtcc tgcagatcat caatgctggc catatccatc agccaacgac cttcccagta
     1801 gtgagtcgtc agtaaaaatg cagggaagcg aatctgcgta tcagccagtt catcggcctg
     1861 tgagaagctc agttcctcgt cgccctcata ccctaaatca aatgccagcc tctgccactc
     1921 ctgaaccgcc tgctgttctt taagaacgat ttcatgggcg gagtcaatct gctgccggta
     1981 tgttgtttgc tcgcgctcag cggaattgag cagcccatca atattacgtt cgatagtttc
     2041 aggatcagac cactgatttc ctgcaatcag cgcgcctaat ttatcttcga gaaacagctg
     2101 tatttggtac tgtcgcttat tgcgaaccgc cgggagccag gaaaataatg aatagatcag
     2161 tgattcaccg gcgcgatatt ttttccattc cgttttagca ctcttcagta gagtgacttt
     2221 ttgttcttgt ccggaaagta atttatttaa attatcgaga tattgctcaa tatcattagc
     2281 aataagctca cgcgcagccc gaatctggct taacgtttgc catgttgcgt tcagtcttat
     2341 cagttgctca gattttgctg ccaactgacc atgcaggagt tcaatgacct tttcagggga
     2401 tgaacactct tttccaggaa aggctgcctt agccttttcc agataaaaca gcagtgcatc
     2461 ctctacatac tcttttgact caacctggtt gaagaaatct tcagtttgat attttttggc
     2521 tgcctcagct ttacgactgc ttgagggaaa ataagcaccg aagcttttca gctctggcaa
     2581 ccatcgcccg gccatcgcac ctgaaccttg cgaaaagtct ttcccgaatg cctcaataat
     2641 gttcgttaca gcctggttat tcgttgaagt cgcgataata accggaggct cagatttttc
     2701 gagagccgct ctggcccact gcgtggcgat gatagaaagc accagcgtgg tttttccggt
     2761 tcccggaggg ccattaacag caaggatatc gccatgtctt gcatccagaa aatggcttaa
     2821 ggcatcgcgc tgagcctttg ccagcggaaa cttatctccg gagtgtccaa gcctgtcgct
     2881 gaattttgct cctggggcca gcaaagactc tgcagcatga acctctcgcg aggcgaagcg
     2941 attgaagagc ggcacatcct tattgcaaac aatcaggtga tcataaagag aaaggatatg
     3001 ggaactggca ccgccagatt gagccgtttt aacaatataa ccgtgctcag cgagttcata
     3061 ttgctcaggt ttttcaatcc agtcgccggc aacgctcttc agtagcctct ctgagtcata
     3121 cagatattga cgccactcct gctgcaaggc ggcatatcgt gcttcccgtt cttcatccgt
     3181 ttcggcagtc ttatcaacgc tgtcatcaaa gttgatagag aacgtggtat gggtcgtctt
     3241 gtatttgtca tactgcccaa tctcaccaat cgaaaatgct ccttttggca aaggttcaag
     3301 caggtctctg ggaatggagg tcgctggcgt cggatataaa aaaccctcac ggcttagcaa
     3361 ggctggcgtc actatcgggg taacaatatc aggcgcacct gcagaacggt ccttaccatg
     3421 ctgcagtaac cggaaataaa cttttgggcg caagatgaca tcgaccgttt cgacatcgtc
     3481 tttttctccc ttaaaaaatt tactgacaat cgcttcgtca agacgtcccg ccgctatccc
     3541 atgccagtga gtgaaatttt tggcgtcttt ccgttcaaaa ctgccctttc ctgactcagc
     3601 atccgcaagc gagttgcgcc agtatgaggc aaaccctaaa gcatttttat ccattaccag
     3661 tcctgtgact caatgaaatc cattgattta tcgaatataa aaccaccata tggacaacta
     3721 ttgtccatat tgaaaaagtt ggatgcaatg ttttgtttat aattcggtat caaatgtact
     3781 ggttgtacaa ggttcagaac acaagcacat tcaacagtac gtcaggacta ttgatttcat
     3841 aacagtgaat acatttttta ccgtttataa tgactgcctg tgttggacaa ggataccaga
     3901 acagccgaaa acaccggggg ttattacctg ctctcatgga aaccagatat tcctggtgct
     3961 aatcacagtc ataaatagcc tgaagaacat gacgaaggga ctttaatgga agccggagta
     4021 acttgccaga acatcaatct tcgtcatcat ttacaaagaa aaataatcag ttaaagcagt
     4081 ccgggcaatc agcccggact gcttactatt ttaatggatt gttaatcttt gcatattcaa
     4141 acgggctgat acccttatct ctgttcgcat ccagaaaatc tgcccaccac tgtagcatca
     4201 gtcgacgttc ttccagatgt tctgctttat ggatatacgc agcacgtaca ccattacgct
     4261 cctgatggct catctgacgt tccacagcat cgcgggacca caaacctgat tcaatcaatg
     4321 cactgcacgc catcgcccgg aatccatggc cacagacatc ctgggttgtg tcatacccca
     4381 tcaccctcag ggctttattt acag
//
LOCUS       DAJTZL010000244         4401 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.246, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000244 DAJTZL010000000
VERSION     DAJTZL010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4401
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.246"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..590)
                     /locus_tag="OO544_003896"
     CDS             complement(<1..590)
                     /locus_tag="OO544_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000785580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HCR6769383.1"
                     /translation="MADSQFYRPGLPQLISMIRSDLLTRFEQDTLLRRMDAEVYARVQ
                     AAAVHTLYGYIDYLARNLLPDVCDEDWLYRHARIKRCPRKEAVAARGFVRWDGVEGTP
                     VLPAGTQIQRDNQVTFTTTATVTAADGLLRVPVVADEPGSAGNTDDGIAMQLGTPVSG
                     LPSTGYADTITDGEDIENLEIWRARVMERYYYIPQGG"
     gene            complement(583..993)
                     /locus_tag="OO544_003897"
     CDS             complement(583..993)
                     /locus_tag="OO544_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000973352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HCR6769384.1"
                     /translation="MMINVNGRPVSTGASIDLLTRAVIISLFTWRRAGRDDAPRIFGW
                     WGDTWPAVQNDRTGSRLYLLRRSKLTNKTPQLARDYVREALAWMVEDGVASRLDINAV
                     RTGTDSLALAITIYQRDGNIHNIIFDDIWSELNG"
     gene            complement(999..1532)
                     /locus_tag="OO544_003898"
     CDS             complement(999..1532)
                     /locus_tag="OO544_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015967647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein"
                     /protein_id="HCR6769385.1"
                     /translation="MNGFSLRNLISRAVITAVDSARKCQSVGLKMIAGDQKQHVEHLE
                     PYGFTSAAQNGAEGVALFPAGDRSHGVVVVVADRRYRLKGLKRGEVALYDDLGQSVVL
                     TRSGIVVDGAGKAIIFKNAPKARFEMPVESTADITDNCDSGGLSMQQMRQAYNAHKHT
                     ENGDGGGITDTPDQPMG"
     gene            complement(1510..2592)
                     /locus_tag="OO544_003899"
     CDS             complement(1510..2592)
                     /locus_tag="OO544_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate protein"
                     /protein_id="HCR6769386.1"
                     /translation="MNDTVLLRVSGREWGGWTSVRISAGINRIARDFNVAITTRWPGS
                     RDYQPRIKNGELVEVLIGDEPVLTGYVEALPLRYDASSVSMGIVGRSKTADLVDCSAL
                     PLQQSGKNLLRIVSELAAPFGITVVDAGVPQTAVIDAQPEHGETVADCLNRLLGQVQT
                     LAYDDECGRLVLGKPGTGKAATALVLVLGENILSCDTERSIRERFSEYQVSGQRPGND
                     DDFGEATIAAIRQTIQDSGVTRYRPLLIQQSGTATTATCKARCEFEARQRAALTRETT
                     YTVQGWRQGSGALWRPGLSVIVFDPLNNFDNDELVIAEVTYNQDDRGTTTELRVGPAD
                     AYLPEPVTARKKKMLRRISDERVFSS"
     gene            complement(2589..3980)
                     /locus_tag="OO544_003900"
     CDS             complement(2589..3980)
                     /locus_tag="OO544_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000347288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization N-terminal domain-containing
                     protein"
                     /protein_id="HCR6769387.1"
                     /translation="MDLPSLPSLSSLFSASSGINWRDNLYDASFRGVPFSVESDEGSF
                     GRRVQVHEYPNRDKPYTEDLGRATRRLTINAYLVGDDYAEQRDRLITAIETAGPGTLI
                     HPQFGEMQGCIDGQVTVSHSGTEGRMCRVSFQFVESGELSFPVAGAATARKLEESSVF
                     LDELIEDMFGDFDLAGIPDFIQNDVIARTTDMLGTVQTAFKMVNSAVSAGARLLQGDL
                     SVILMPPSVASDFVHTLQDTWRAGTRLVDNTQDLVQSITTMSGITLDPGLAPRAVWPT
                     DSASVIRQKQQTNLVAAVIRTTAISEATRAVTSLPQPGSLVKNQQAVVAVGGSTERQS
                     DIIHVSHPALDSVAASTGQDERAQPPTRENLTIIRKSLNAAIEQELRRTADDRLFFQL
                     TSLRTELNRDIQARLVQTEETAERTPAEVLPALVLAASWYDDASRETDILDRNAISHP
                     GFVPVRALRVPVR"
     gene            complement(4027..4233)
                     /locus_tag="OO544_003901"
     CDS             complement(4027..4233)
                     /locus_tag="OO544_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6769388.1"
                     /translation="MTFLIPIYKDDDFDSDTVGFTFAFKMPRGQFFVDVKENGNIRAG
                     VNVNGESGVTYENCKLNMKDINDD"
BASE COUNT         1054 a         1283 c         1040 g         1024 t
ORIGIN      
        1 cccccctgtg gaatgtagta ataacgttcc ataacgcggg cacgccatat ttccagattt
       61 tcaatatctt caccgtctgt aatggtgtca gcgtaccctg tggacggcag accactgacg
      121 ggtgttccca gttgcatggc aataccatca tccgtattcc ccgccgatcc cggttcgtct
      181 gccacaacag gcacccggag aagaccatcg gctgcggtca ccgtcgccgt cgtggtaaag
      241 gtcacctgat tatcacgctg gatctgcgtt cccgctggca ataccggcgt cccctctacg
      301 ccatcccagc gcacaaatcc ccgggctgcc accgcttctt ttcgcgggca gcgtttgatt
      361 ctggcgtgcc ggtacagcca gtcttcatca cacacgtccg gtaacagatt tctggcaaga
      421 taatcgatat acccgtacaa cgtgtgtacg gctgcagcct gtacacgggc atacacttcc
      481 gcatccatac gacgaagcag cgtatcctgc tcaaagcggg ttaataaatc gctccggatc
      541 atagaaataa gttgcgggag gccggggcga taaaactgac tgtcagccat tcagttcact
      601 ccagatatca tcaaaaataa tgttgtgaat attgccgtca cgctggtaaa tggtaatggc
      661 aagtgccagc gagtctgtcc cggtccggac agcgttaata tcaagacggg aagcaacacc
      721 atcctccacc atccacgcca gcgcctcacg gacataatct ctggcaagct gcggggtttt
      781 atttgtcagc ttgctgcgtc gcaacagata caaacgcgac cccgtgcgat cattctgaac
      841 cgcaggccag gtatcccccc accatccaaa tatccgtggt gcatcatcac gcccggcacg
      901 ccgccatgta aaaagcgaaa taatcacagc acgcgtcaga aggtcgatcg aagccccggt
      961 cgacacgggt cgcccgttaa cattaatcat catgattttc agcccatcgg ttgatccggc
     1021 gtgtcagtga tcccgccacc atcaccattt tcggtatgtt tgtgggcatt gtaggcctgc
     1081 cgcatttgct gcatgctgag tccgccactg tcgcaattgt cagtaatatc ggcggtggat
     1141 tcgactggca tttcaaagcg cgctttaggc gcgtttttaa aaattatggc cttcccggcc
     1201 ccgtccacca caataccgga acgggtcagg acaacggact gtcccagatc gtcataaagc
     1261 gccacttccc cacgtttcag tcctttcagc cggtaacgtc tgtcagccac gaccacaacc
     1321 acaccatgag aacgatcgcc cgccggaaat aaagcaacgc cctcagcacc gttctgtgcg
     1381 gcagatgtaa aaccataagg ttcaaggtgc tcaacgtgtt gtttctgatc tccggctatc
     1441 attttcaacc ctacagactg acactttctg gcggaatcca ccgccgtgat gacagcccgg
     1501 gaaatcagat tacgaagaga aaacccgttc atcagaaatc ctcctcaaca tttttttctt
     1561 cctggcggta acaggctcgg ggagataagc atctgccggg ccaacccgta attcagtcgt
     1621 cgtgccccgg tcgtcctggt tataggtaac ttctgcgatc accagttcat cattatcaaa
     1681 attattcagc gggtcgaaaa cgatgacaga taaccccgga cgccataacg cgccactgcc
     1741 ctgccgccag ccctgaaccg tatatgttgt ctcacgggta agcgcagccc gttggcgcgc
     1801 ttcaaattca caacgggcct tacaggttgc tgtcgttgct gtgcctgact gctgaatcaa
     1861 caaagggcga taacgggtca cgccactgtc ctgaatggtc tgacgtattg cggcaatggt
     1921 ggcctcacca aaatcatcgt cgttgcccgg gcgctgccca ctgacctgat attcagaaaa
     1981 ccgctctctg atgcttcttt ccgtgtcaca ggaaagaata ttctctccca gcaccagcac
     2041 cagcgccgtc gccgctttac ctgttccggg ttttcccaga accagtcgcc cgcattcatc
     2101 gtcataagcc agcgtctgaa cctgccccag taaccggtta agacaatcgg caacggtttc
     2161 gccgtgttcc ggctgggcat caatcaccgc tgtctgcggc acgccagcat caacaacggt
     2221 gatgccaaat ggcgcagcca gttcactgac tattctgagc aggttttttc cgctctgctg
     2281 gagtggcaaa gcagagcagt caaccagatc ggctgttttg cttcgcccga caattcccat
     2341 gctgacgctg ctggcgtcat aacgaagcgg tagtgcctcc acatatccgg tgagcacagg
     2401 ctcatccccg ataagcactt caaccagctc accatttttt atccggggtt gataatcccg
     2461 gcttccgggc cagcgggtgg taatggcaac attaaaatcc cgggcaatac ggttaatgcc
     2521 cgcactgata cggacggatg tccagccgcc ccattcgcga ccggaaaccc gaagtaaaac
     2581 ggtatcattc atctgacggg tacccttaat gccctgaccg gaacaaagcc cggatgagag
     2641 atggcatttc gatccaggat atcagtttca cgggacgcat cgtcgtacca tgacgcagcc
     2701 agaacaagcg caggcagaac ttccgctggc gttcgctctg cggtttcctc cgtctgaacc
     2761 agacgcgcct gaatatcccg gttcagttct gtacgtaatg acgtcagctg aaaaaacagc
     2821 ctgtcatccg ccgtacgcct gagctcctgt tcaatcgccg cgttcagcga tttgcgtatg
     2881 atggtgagat tttcccgcgt gggtggttgc gctctctcat cctgtcctgt gctggctgcc
     2941 acgctgtcaa gtgccggatg agaaacgtga ataatatcgg actgacgttc agtggaacca
     3001 ccaacagcca caaccgcctg ctgatttttc accaggcttc cgggttgtgg cagtgaagtg
     3061 accgcccttg tggcttcgct gattgccgtc gtccggatga cagcagccac aaggtttgtc
     3121 tgctgttttt gcctgataac cgatgcggaa tctgtgggcc acaccgcacg cggtgccagt
     3181 cctgggtcca gcgtaatgcc ggacattgtc gttatggact gcaccagatc ctgtgtgtta
     3241 tccaccagcc gggttccggc ccgccaggta tcctgcaacg tatgcacaaa atcactggca
     3301 accgacggcg gcatcagaat gacggataaa tcgccctgca gtagtctcgc tccggcagaa
     3361 acagccgaat taaccatttt gaaagctgtc tgaacggttc ccagcatatc ggtcgtccgg
     3421 gcaataacat cgttctgaat aaagtccggt attcccgcga gatcaaaatc gccgaacatg
     3481 tcttcaatca actcatccag gaatacggac gactcctcca gttttctggc ggttgcagct
     3541 ccggcgaccg gaaatgacag ttccccgctc tcaacaaact gaaatgaaac ccggcacata
     3601 cggccttcag tgccggagtg agaaacggtc acctgcccgt caatacagcc ctgcatttca
     3661 ccgaactgcg gatggatcag cgtccccggc ccggcggttt caatcgctgt aatcagcctg
     3721 tcgcgctgct ctgcgtaatc atcaccaacg agatacgcat taatcgtcag ccgtcgcgtg
     3781 gcacgcccga gatcttccgt gtacggtttg tcacggtttg ggtattcatg gacctgaacg
     3841 cggcgtccga acgaaccctc gtcgctctcc accgaaaacg gaacgccacg aaatgaagcg
     3901 tcgtaaagat tatcgcgcca attgatacct gaagatgcag aaaagagtga agaaagagaa
     3961 ggtaaagaag gtaagtccat actgtctcca ctataaaaaa accgccaatt tctggcggct
     4021 tatatattaa tcatcattaa tatctttcat attcaatttg caattttcgt aggtcactcc
     4081 cgactcgcca ttaacattaa ctccagctct gatattcccg ttttctttta catctacaaa
     4141 aaactggcct cttggcatct tgaacgcaaa tgtaaaacca acagtatctg aatcaaaatc
     4201 atcatcttta tatattggga tcaaaaatgt catcgtcgct ggtgatgcat tgtttgcaaa
     4261 atcaaataca cctatacttt tgccgaattc ataagaagct tttctcaata taatcgcacc
     4321 gctggcagaa ggggatgggc acgatatcct cagttgtctg aatccatctg tatccaagtc
     4381 aacgtcatta tctgcagatg t
//
LOCUS       DAJTZL010000245         4325 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.247, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000245 DAJTZL010000000
VERSION     DAJTZL010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4325
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.247"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..579)
                     /locus_tag="OO544_003902"
     CDS             complement(<1..579)
                     /locus_tag="OO544_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR6769389.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIDLGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYTSYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADAITVDAMIPN"
     gene            complement(642..1091)
                     /locus_tag="OO544_003903"
     CDS             complement(642..1091)
                     /locus_tag="OO544_003903"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HCR6769390.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(1088..4180)
                     /gene="ebgA"
                     /locus_tag="OO544_003904"
                     /pseudo
     CDS             complement(1088..4180)
                     /gene="ebgA"
                     /locus_tag="OO544_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026199.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
BASE COUNT         1027 a         1089 c         1167 g         1042 t
ORIGIN      
        1 gttcgggatc atcgcgtcaa cggtgatggc gtcagcaggc tgtacgccgc caaccagcgc
       61 cgtacctgcc agtaaaatgt aggagagcgt taacagcagc atcagcgttg aagtgacgga
      121 ggtaattggc cccaacattt tcgccccgtt ggtggaaacc caggtggaga aggcgaacag
      181 caccatactg ataatggtgg tggcaaccgg cgtcataata tattcgtatc cgaggaaggc
      241 atacgaagta taagcaataa cgcgcggcaa cagtgaggtg aaaaagaaca ggtttacgaa
      301 ccagtaggta taggcagtaa taaatgccca acgtccgccc agcgaacttt ttacccatgc
      361 gtagacaccg gcttctgagt ttttatttaa cgaaacaaat tctgcgatga tcagacagaa
      421 gggaataaaa taaaaaatcg tcgcgaggaa aaacatcggt gccgaggcca gtccaagatc
      481 aatattatta ttgataacgt tgttaaagct gtaaacggcg gcaaaagtca gcgagagcaa
      541 gccgaatttg ccgattgtat tacgtttggt atcagacata acacatctcc gaaaatgagt
      601 agggtagcgg tggcggcaaa ctccttttgc cgccgtagtt gttatttgtt atggaaataa
      661 ccatcttcga tggtgacttt gagaaccact tttttgaccg cgttattgca gataaaccga
      721 tacgcttcat ggatatcgca aataacgatt tgcccttcgt ggacctcaac ggtttctccg
      781 cagcctttta aatattcacg gtcagtttca tcacgataat attccactac ctgtaatgtc
      841 tctttcggcg catattcaat tttttgctgc ccttgcaggt agtaatgcac ttcaaaatag
      901 cgtcgatgcc cggtaaatag cgcatcggtc gcggagtctg tctccacgcg gtaagtcaat
      961 gagtcaccga tggagtgggc gacgccaggc tgaatgttgt cgatattttc aatcgcttca
     1021 acgcagcgtt gccacttctt gccagaggcg taaatctggc ggaactgttc taagttatcg
     1081 atgatcctca ttgcttattc tcgctgtgca aattcgtgga aaagaaccct gcgccgaact
     1141 catacgacgc caggctttgc gcggtagctt ctccgccaga aaccggcagc aacgtaaagc
     1201 cgtagctgaa gtcacggaac cagacgcgcc aggagtccag cacctcgctg ccccaggagt
     1261 tggagccgag gccaagcagc tggtgatcga ggttcagggt gatgtcatca ctgcgctgca
     1321 gctcgttaca gtgctgggca gcgtggatgt tttcctaggt atagtgccag gcgctgaagt
     1381 taattgggcg ctgcggaacc accagcagac cgttaccgtg gcggttagtc agtgccgtcc
     1441 agcggacatg ctgacggtta ccgttgttct gcgggaaggg atagttctcg aacatggcat
     1501 cgacggtgct gcgccagata tcgatgatgt tagcctgctg gctgtcggcg tagttttcgc
     1561 ccggtccacg accgtaatac gccacctgat cgtattcgcc gttaattccc atggtgaaac
     1621 cgatgcacgg aatgatgtgc ggatagtcgc cgtaacgctc gccggaaagc gccacgttaa
     1681 cctggccatc ggcagcgatg cgccagatgt aggtgcagcg cattccgaaa tcaaacaccg
     1741 gcggggcaat aaccgtgcgg ctgatgatca acacttcacc ctcgctctgt tctacggcaa
     1801 agtcgcgcag atgttcctgc atgatctgca aatgattcgg ctgccacaac ccttcgtact
     1861 cctgcttgtg gttgtcgatc atcggcttga agaagttgat ctttggctcg cgggtcagca
     1921 gcgactcgcc atttacctgc caggatgtcg gtttgccact catttttgag aaggtgatcg
     1981 cgaagttgta gccgcgaacg gtgcagctca aacgatcgtc ttccagcgtc agcggacgcg
     2041 cattatttgg tgcgaaaggc actggctgcg cggtgttttc cttcagcggg aactgataag
     2101 tggcgataga atgcccggct tcgctgtagc gggtgcggga atctttggtc accgtaatgt
     2161 tgaggaacgc ttcgcgggcg tccagctgcg gcagcgtgat ctgcaagggg gcttcgctgt
     2221 tcggcgcaac gtcgcgcagt ttaatctgct gcgtcgcgag cgtttcacct tcggcgcgca
     2281 cctctgcgtg cagggggtag tcatcaagcg tggtaaacca cagtttattt tcgactttca
     2341 actcgccgcg agtcagatcc cgcgcgtgga tttttaccgg cgcgataacc tgtttgtact
     2401 ctttcaggcc cggtcccggc gtctgatcgg aatagatcaa accatcaaga cagaagttat
     2461 agttgttggg atagtcgccg tagtcgccgc cgaatttata ccagacattg ccgtggtcgt
     2521 cctgtgcctg gatcccgtgg tcgcaccact cccagacata atgaccctga atgcaatcgt
     2581 gcttatagaa gacgttctgg tactccgtca gcccgcccgg tccgttcccc atcgcatgag
     2641 catattcaca gatgatgcgc ggcttcggat gcgggtattc accaaactca ttcatcagcg
     2701 gcacgcgggt gtacatggtg gaaataatat cgaccacttc agcatcgcga tcttcttcgt
     2761 aatgcaccag tcgcgtgtca tccagcgcct tcgctgcgtg gtacatcgcg cggatgttac
     2821 agccatagcc ggattcattg cccagcgacc agatgatgat cgacggatgg tttttctgcg
     2881 cgtgaatatg gcgaacaatg cgctcgacgt agactttttc ccactgcgga tcgtcggtaa
     2941 tacggctgat atcgccgaca ttagcaaagc cgtgcgattc gacgtcggtt tccgccatca
     3001 caaacaggcc gtagatatca cacagttcgt aaaaacgcgg atcgttcggg tagtgagcgg
     3061 tacgcacgga gttgatgttg tgctgcttca tcaactggag atctttctcg acgcgatcca
     3121 ttccaacggc gcggcctttg cgatgatcgt tgtcgtgacg gttgacgccg tgcagcatca
     3181 cataacggtt attgatccag aacagaccgt cgcgcacttt gatatcacgg aagccaacgc
     3241 gttgtggtac cacttccaga acgttgccgt tggcgtcttt cagcgtcatg accagatggt
     3301 aaagataagg ggattctgct gaccattgct gcggctgttc gacagtaaaa gcaaagctgg
     3361 cgctggtcag tttttcaatt gccaaatgat caatggcgct gctgtgcacc acgcgttcgc
     3421 catcaaacag ggtatattcc agcgtcgtga cgacagggga ggcggcgaga ttttccagca
     3481 ccacttcgca ggaaagcgtg gcatcgcaat aggcttcgtc aaagtcggta cgcacggtga
     3541 aatcgttaat atgtgttagt tgttttccga tcagataaac atcgcggaag atccccgctg
     3601 accaccacat atcctggtct tccacgtagg tagagtccgc ccattgcatc acgcgcacac
     3661 acaacaggtt gtcgccggtt ttaaccatcg cgctgatgtc aaactctgcg gtcaggcgac
     3721 tgcccttgct gaaacccaca tactgaccgt taacgtagac ttcaaaatag gtttcgacgc
     3781 cgtcaaattt aatcagcgtc tgtttaccct gccagccgtc gctgagggtg aaaatacgtt
     3841 gataggcacc ggttgggtta tcgctgggaa caaacggcac atcgatgggg aacggaaaac
     3901 cttcgtcggt atattgcagt ttgccgtgac cttccatttg ccacatggcg gggacggtaa
     3961 tatgccccca gtcagccatt aactcagagg tgaaggcttc aggcacttgc agcgggtggt
     4021 caaaaaagtg gaaattccac tgaccgctta agggcagaaa caggctgctg gtttcgcggg
     4081 caaaggtacg cgcttgcgca acagaatcat atgaaaaaaa gtacgcacgt ggcgcaagtc
     4141 ggttttcgtg ggtgagctga atgttttccc agcgattcat agggcctccc ggtagagaga
     4201 aatcggaacc attcatggtg atggtagttt gtgtaattgc tggggagttt agtaaaattt
     4261 ttactaaaaa gtgacaggta aatgcattta ctttaactgg atcacgaaaa taaacggcag
     4321 cactg
//
LOCUS       DAJTZL010000246         4324 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.248, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000246 DAJTZL010000000
VERSION     DAJTZL010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4324
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.248"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..1882)
                     /gene="speC"
                     /locus_tag="OO544_003905"
     CDS             complement(<1..1882)
                     /gene="speC"
                     /locus_tag="OO544_003905"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HCR6769391.1"
                     /translation="MKSMNIAASSELVSRLSTHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGATSVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKAELPRPFR
                     LAIIQLGTYDGTVYNARQVIDTVGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSPLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAD"
     gene            2284..2991
                     /locus_tag="OO544_003906"
     CDS             2284..2991
                     /locus_tag="OO544_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HCR6769392.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            3097..3172
                     /locus_tag="OO544_003907"
     tRNA            3097..3172
                     /locus_tag="OO544_003907"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3130..3132,aa:Phe,seq:gaa)
     gene            3370..>4324
                     /locus_tag="OO544_003908"
     CDS             3370..>4324
                     /locus_tag="OO544_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HCR6769393.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDEARKLISEGVDPCEQKRAKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSNKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALDLSRLPELLSRIGS
                     YKGQPVTQLAVMLNLLVFIRSSELRYARWSEIDIDNAMWTIPAEREPLPGVKFSHRGS
                     KMRTPHLVPLSKQAVAILTELQTWAGENGLIFTGAHDPRKPI"
BASE COUNT         1118 a         1145 c         1040 g         1021 t
ORIGIN      
        1 tatcagcgct atgcgcatcc tgggggttca tcaccactga cgggaaactc tgctggcgga
       61 acatcgcttt ttgtaggtct ttgacgtcga aactgacata cagatcgtgc atctcctgac
      121 acaactggcg cagggtgtag tcgcgatagc gcaccggata tttgttataa acgctcggca
      181 acacctcagc cagcggcgag tcatcctcaa tatgctgttc aaattgcgcc agcatcgcca
      241 ccagttgtgc cagcttctcg tggctttccg ccggagttaa taagaacaga atggagttga
      301 gatcgcactt ctccggcaca atgccgttct cacgcagata gtgcgccaga atcgtcgccg
      361 gaacgccaaa gtcgctatat tcgccggttt cggcattgat acctggtgta gtgagtaaca
      421 gcttgcacgg atcaacaaaa tactgatccg cggcatatcc ttcaaagccg tgccacttcg
      481 cccccggctc aaaactgaaa aaacggcggt cgctggctaa cacagatgtc ggataatcct
      541 gccacaattt gccatcaaca acgggcggga taaacgggcg gaacagctta cagcgcgcaa
      601 gaatagcctt gcgcgactca atacccaact caacgcactc agcccacagc cgacgcccac
      661 tctccccttc atgaattttg gcgttaacat ccagtgcagc aaacagcgga tagaaagggc
      721 tggtagaagc atggagcata aaggcgttat tcaaccgctt atgcgggcaa aaacgcgcct
      781 gtccgcggat atggttatct tttttatgga tctgcgacgt ctgtgagaat cccgcctgct
      841 gtttgtgcac cgactgagtc acaaagatcc ccggatcgtt ttcgttaagt tctaatagca
      901 gcggcgagct atccgccatc atcgggataa actgttcgta accgacccac gcggaatcaa
      961 acagaatgta atcacacaga tgcccaacgg tatcgatcac ctgacgggcg ttatagacag
     1021 ttccgtcata ggttcccagc tgaataatcg ccaggcgaaa cgggcgcggc agctcggctt
     1081 tttctggcgc aacgtcgcga atttgctggc gcagatactc ttcattaaaa cagtgcgcat
     1141 caataccgcc aatgaaacca aacgggttgc gtgaagcttc cagatagacc gacgtcgccc
     1201 ccgcctgaat cagcgcgccg tgatgattcg acttatggtt gttacggtcg aagagcacca
     1261 gatcgccacg cgttaacagc gcattcgtca ccactttatt cgctgccgat gtgccgttca
     1321 gcacaaaata ggttttatcg gcatgaaaga ctttggctgc gaatttctgc gcatccttcg
     1381 ccgatccttc atgaataagc agatcgccca attttacgtc agcgttacac atatcggcgc
     1441 gaaagacgtt ctcaccaaag aaatcgtaaa aatggcgtcc ggcaggatgc tttttaaaaa
     1501 acgcaccatg ttgatgtcca gggcaagcaa aggtgctgtt gcccatctca acgtactgcg
     1561 tcagcgtgtc ataaaacggt ggcagcaaat tctcttcata ctgacaggct gcggattcca
     1621 gctccagcca ctgctgctcg ttgccgttga ttaccgccgt aacgcccgca ggtaattcaa
     1681 cagcatgttc ggaatacaaa aacaccggta gatgaaaacc ggtgcgctta agcaacgcaa
     1741 gaatgccact gcgactatcc gcagcggtaa tgacgactgc cgcgacatcc gtaaaatcag
     1801 tatctcccaa cgccaccacg cggcgatgag tagaaagtcg ggataccagt tcactactgg
     1861 cggcaatatt cattgatttc ataagcgcaa aacccgtttc ggggaagtaa gaataccgga
     1921 caaagtgtaa aaccctgccc catgagatat gggtcaaact ggtcaccagc tccgacgacc
     1981 gccagacatc agtaaaagca gaaacgctct gattttactg ttgtcctgca gtgaacgtgc
     2041 gttaacttca ccgcatgagc agtaacatag aaagggaaac gtttcgcgcg aaacggcgat
     2101 aagcgagaga atgtaaggag atggcgtgca tcgggcaaac tccgtacaga gaggagaaaa
     2161 ttcgcgcaat catggcacct ttcgctaagg cgtgcaagcc aggactttgc gaccagacaa
     2221 gccatcaaca aggcaaaaag ttcataataa gaaaatcaaa caatacagct aacaggaact
     2281 cttgtggtca tcggcccttt tatcaacgct agtgcagtct tactgggtgg cgttctcggc
     2341 gcactgctca gccaacgctt accggaacgt atccgcgtct ccatgacatc tatttttggt
     2401 ctggcatcgc tggggattgg tattttactg gttgtgaaat gcgccaacct tccagcgatg
     2461 gttttagcta cgctacttgg cgctctaatt ggcgaaattt gtttgctgga gaaaggtgtc
     2521 aatacagcag tcgccaaagc acaaaatctg tttcgtcact cacgtaagaa gccagcgcat
     2581 gaatctttta ttcagaatta tgtcgcgatt attgtcctgt tttgcgccag cggcaccggg
     2641 atcttcgggg cgatgaacga agggatgacc ggcgatccga gtattttaat cgccaagtca
     2701 tttcttgatt tctttacggc gatgatcttc gcctgctcgc tgggtattgc ggtgtcggta
     2761 attagtatcc cattactgat catccagcta acgctagcgt gggctgccgc gctgatatta
     2821 ccgctgacca caccgtcgat gatggcagac ttcagcgccg taggcggttt attgctgctg
     2881 gcaaccggat tacgcatctg tggcattaaa atgttcccgg tggtcaacat gcttccagca
     2941 ctccttctgg caatgccgct ttccgccgcc tggaccgcct ggtttgcctg acaatgcgtg
     3001 caatagcggc aaagtgatga tagattgtgc agtctgcagt aaattgaaga aatttgattg
     3061 acgagacgag gcgaatcagg tttaatgcgc cccgttgccc ggatagctca gtcggtagag
     3121 caggggattg aaaatccccg tgtccttggt tcgattccga gtccgggcac caaattcata
     3181 tcaacggacc tccacggagg tccgtttttc gtttcagaac gccacgattt aagcgttctg
     3241 cctacaaatc aattctaccg aactcaacca gattcccccc acatcaaccc cattgtgtgg
     3301 gtataattgc gggcataccc cagttcgaca gaatttgtac cctctttaac gcattcagaa
     3361 ggctgaacta tggcactgac tgacgcaaaa atccgggctg caaagcccac tgacaaggct
     3421 tataaactca ctgacggggc tggcatgttc ctgctggtac atcctaatgg ctcccgttac
     3481 tggcgtctcc gttatcgtat tctgggtaag gagaagactc tggcacttgg tgtgtatcca
     3541 gaagtttctc tctccgaagc tcgtacaaaa cgggatgagg cccgaaaact gatttcggag
     3601 ggggttgacc cttgcgaaca gaaaagagct aaaaaagtag tccctgattt acagctctct
     3661 tttgaacata ttgcgcgacg ctggcatgcc agtaataaac aatgggcaca atcacacagc
     3721 aataaagtac tcaaaagcct tgagactcac gttttcccct ttatcggcaa ccgggatatc
     3781 acaacactca ataccccgga tctgcttatc cctgttcgtg ctgcagaagc taaacaaatt
     3841 tatgaaatcg ccagtcgtct gcagcaaaga atatctgccg taatgcgtta tgccgtacag
     3901 tctggcatca tcagatataa tcctgctctg gatatggctg gcgcattgac tacggtaaaa
     3961 cgccagcatc gccccgctct tgatctttct cgcctgcctg aacttttgtc gcgtattggc
     4021 agttacaaag ggcaacctgt cacccagctt gccgttatgc tgaatttact ggtttttatt
     4081 cgttccagtg aactcagata cgcccgctgg tctgaaattg atattgacaa tgccatgtgg
     4141 actattccag ccgaacgcga acccctgcca ggcgtaaaat tctcacaccg gggctccaag
     4201 atgcgaacac cacatcttgt gccactcagc aaacaggctg tagccatact gacagaacta
     4261 cagacatggg ctggtgaaaa tggtctgatc tttacgggag cacatgaccc gcgtaaacca
     4321 atca
//
LOCUS       DAJTZL010000247         4262 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.249, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000247 DAJTZL010000000
VERSION     DAJTZL010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4262
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.249"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            288..896
                     /locus_tag="OO544_003909"
     CDS             288..896
                     /locus_tag="OO544_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HCR6769394.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            994..2385
                     /gene="selA"
                     /locus_tag="OO544_003910"
     CDS             994..2385
                     /gene="selA"
                     /locus_tag="OO544_003910"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HCR6769395.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRDSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPMIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            2382..4226
                     /gene="selB"
                     /locus_tag="OO544_003911"
     CDS             2382..4226
                     /gene="selB"
                     /locus_tag="OO544_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HCR6769396.1"
                     /translation="MIIATAGHVDHGKTTLLQVITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPSFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPGREHASQHSVRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETAHAGQRIALNIAGDAEKEQINRGDWLLADAPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQSDADALSVHLERGAVNLADFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
BASE COUNT          964 a         1078 c         1241 g          979 t
ORIGIN      
        1 acaatgctac cgccatactg cgtcatgtcg ccctgacgcg ctgccggttt tccgctcatc
       61 tgctcatcct tgttttatcg ggttatttgt ctgcttccag gcaaaaccct acagatttat
      121 aagaaagcct taagtgagat atccatcaga tagaaacaat taggaaaata tttatatcac
      181 catgataaat atgtgattta tttggaagta acaaaccatc aacattttct gccctttttc
      241 ccatcaccag gccatactga tatttttcct cgcacaaagg actcggcatg aaactcgtcg
      301 gtagctacac cagcccgttt gtacgcaaac tttctattct gttgttagaa aagggcataa
      361 ctttcgaatt tattaatgaa ctgccctata acgcggacaa cggcgtggcg caatttaacc
      421 cgttaggaaa agtgccggtg ctggtgaccg aagagggcga atgctggttt gattcgccga
      481 tcatcgctga atatattgag ttaatgaatg tcgctccggc gatgttgccg cgcgatccgc
      541 tggagtcgtt gcgggtgcgc aaaattgagg cgctggcgga tggcattatg gatgccgggc
      601 tggtatcggt gcgtgaacag gcgcgtccgg cggcgcagca gtctgaagat gaattgttac
      661 gccagcggga gaaaatcaac cgcagtctgg atgtgctgga aggatatctg gtcgatggca
      721 cactcaaaac cgatacggtc aatctggcga ctatcgccat tgcctgtgct gtcggatatc
      781 tcaatttccg ccgcgttgcg ccaggttggt gtgtcgaccg cccacactta gtcaaactgg
      841 tcgaaaacct gtttagccgc gaaagttttg ctcgcaccga accgccaaag gcttgatgcg
      901 cgatatgtcc tcctgaccca tctcacgtta caatccgtgg ttatgttaaa cgcccttctc
      961 cgtgtgagag ggccttgatc agccaggttt cctatgacaa ccgaaacgcg ttccctctat
     1021 agtcaacttc cggctattga tcgcttattg cgcgatagct ccttcctttc tttgcgtgat
     1081 acttatggtc acacccgcgt ggtggaattg ttgcgtcaga tgctcgacga agcgcgagaa
     1141 gtgattcgtg acagccagac gcttcctgcg tggtgtgaaa actgggcgca agaagtcgat
     1201 gcccggttga cgaaagaggc gcagagcgcg ctgcgtccgg tgatcaacct gacgggaacc
     1261 gtgctgcata ccaaccttgg acgagcttta caggcggaag ccgcggtgga agccgttgcg
     1321 caggctatgc gttcgccagt gaccctcgag tacgatctgg acgacgccgg acgcggacat
     1381 cgcgatcggg cgctggcgca actgctgtgc cgtattacgg gggcggaaga tgcctgtatc
     1441 gtcaataaca atgcggcggc ggtgttattg atgttggcgg ccactgccag cgggaaagag
     1501 gtggtagtat ctcgcggcga attggtggag attggcggcg cgtttcgtat tcccgatgtt
     1561 atgcgtcagg caggctgcac cctacacgaa gtagggacca ccaaccgcac gcacgcgaat
     1621 gattatcgtc aggcggtgaa tgaaaatacc gcactgttga tgaaagtaca taccagtaac
     1681 tacagcattc aggggttcac caaagcgata gatgaagcgg aactggtggc gctcggcaaa
     1741 gagctggatg ttcccgtagt gactgattta ggcagtggct cgctggtcga tcttagccag
     1801 tacggtttgc cgaaagagcc aatgccgcag gagttgattg cggcgggcgt cagtctggtg
     1861 agcttctccg gcgacaagtt gttaggcggg ccgcaggcag ggattatcgt tggtaaaaaa
     1921 gagatgatcg cccgcctgca aagccacccg ctgaagcgtg cattacgcgc ggataaaatg
     1981 accctcgcgg cgctggaagc cacgttgcgt ctttatttac accctgaagc tctgagtgaa
     2041 aaattaccga ccctgcgcct gcttacccgc agcgcagagg tcattcaaat ccaggcacaa
     2101 cgtttacagg ctccccttgc cgcacattac ggcgcggagt ttgcggtaca ggttatgcca
     2161 tgtctttcgc agattggcag tggttcgctg ccggttgatc gcctgccgag cgcggcatta
     2221 acgtttacac cccatgatgg acgcggtagc caccttgagt cattagccgc ccgctggcgt
     2281 gaattgccag tgccgatgat tggtcgtatt tatgacggac gattgtggct ggatttacgc
     2341 tgccttgaag atgagcaacg gtttttggag atgttgttga aatgattatt gcgactgccg
     2401 gacacgttga ccacggcaaa acaaccttat tgcaggtgat tactggcgta aatgctgacc
     2461 gtctgccgga agaaaaaaag cgcggcatga ccatcgatct cggctatgcc tactggccgc
     2521 agccggatgg tcgcgtgcct agttttatcg acgtacccgg tcatgaaaag tttctttcca
     2581 acatgctggc gggcgttggt ggtatcgatc acgcgctgtt ggtggtggcg tgcgatgacg
     2641 gcgtgatggc gcagacccgt gagcatctgg cgattttgca gctgaccggt aacccgatgc
     2701 tgacagtggc gctgaccaaa gccgatcgcg tggacgaagc gcgtgttgat gaggttgaac
     2761 gccaggtaaa ggaggtgctg cgggaatacg gttttgctga ggcaaaactg tttatcaccg
     2821 cagcaaccga aggtcgggga attgatgccc tgcgcgagca tctgcttcag ttaccaggac
     2881 gtgaacacgc cagccaacat agtgtccgcc tcgcgatcga ccgcgcattt accgtaaaag
     2941 gtgccggtct ggtcgtcacc ggtacggcgt taagcgggga agtgaaggta ggcgattcac
     3001 tctggctgac tggtgtaaat aaaccgatgc gtgtacgtgc gctgcatgca caaaaccagc
     3061 caacagaaac cgcccatgcc gggcagcgta tcgcgcttaa catcgcgggt gatgcggaaa
     3121 aagagcagat taaccgtggc gactggctgc ttgccgatgc gccgccagag ccgttcacac
     3181 gggtgattgt cgagcttcaa acccatacgc cgctgaccca gtggcagccg ctgcatattc
     3241 accacgccgc cagccacgtt acgggacgcg tttcgctgct ggaagataac cttgccgagc
     3301 tggtgttcga cacgccgctg tggctggcgg ataacgatcg cttagtgctg cgcgatatct
     3361 cggcccgcaa cacgctggcg ggggcgcgtg tggtgatgct taacccgccg cgtcgcggta
     3421 aacgtaagcc ggaatatctg caatggctgg cgtctcttgc acgggcgcag agcgatgccg
     3481 atgcgttatc tgttcatctg gaacgcggcg cggttaacct tgcggatttc gcctgggcgc
     3541 gccagctcaa cggcgaaggg atgcgcgaat tgctgcaaca gcctggctat attcaggctg
     3601 gttatagctt gttgaatgcg ccggttgccg cccgctggca gcggaaaatt ctcgacacat
     3661 tagcgactta tcatgagcaa catcgcgatg aacctggtcc tgggcgcgaa cgtctgcgac
     3721 gtatggcgtt gccaatggaa gatgaagcgc tggtactgtt gctgattgaa aagatgcgcg
     3781 aaagcggcga catcctcagt catcacggtt ggctgcatct gccggatcac aaagcgggct
     3841 tcagcgaaga gcagcaggcc atctggcaaa aagcagagcc gctgtttggt gacgagccgt
     3901 ggtgggtgcg tgatctggca aaagagacgg gtaccgacga gcaggcaatg cgcctgacgc
     3961 tacgccaggc ggcgcagcaa ggaataatta ccgcgatcgt caaagatcgt tattaccgta
     4021 acgatcggat tgtcgaattt gccaatatga tccgcgatct cgatcaggag tgtggttcaa
     4081 cctgcgcggc ggatttccgc gatcgcttag gcgtaggccg aaagctggca attcagattc
     4141 tggaatattt tgaccgcatt ggctttacgc gtcgtcgtgg aaatgatcat ttattgcgcg
     4201 acgcattatt atttccggaa aaataaggaa atgattaatt aatttttaaa ataaattaat
     4261 ac
//
LOCUS       DAJTZL010000248         4246 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.250, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000248 DAJTZL010000000
VERSION     DAJTZL010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4246
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.250"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..1358
                     /gene="dcuC"
                     /locus_tag="OO544_003912"
     CDS             <1..1358
                     /gene="dcuC"
                     /locus_tag="OO544_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HCR6769397.1"
                     /translation="VVVIVGVARYIIKGYSATGVLFVGGLLLLIISAIMGHKVLPSSQ
                     ASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMVVKLASKPLQY
                     INSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRGAAAAICASPA
                     AIILAPTSGDVVLAAQASEVSLIDFAFKTTLPISIAAIIGMAIAHFFWQRYLDKKEHI
                     SHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVICMLIASILEF
                     LRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQSLISIATSFG
                     SASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAYLTIPMLQASN
                     LGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELMVPGTAAAVTG
                     K"
     gene            complement(1399..2079)
                     /gene="dpiA"
                     /locus_tag="OO544_003913"
                     /gene_synonym="citB"
     CDS             complement(1399..2079)
                     /gene="dpiA"
                     /locus_tag="OO544_003913"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HCR6769398.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISCTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(2048..3706)
                     /gene="dpiB"
                     /locus_tag="OO544_003914"
     CDS             complement(2048..3706)
                     /gene="dpiB"
                     /locus_tag="OO544_003914"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HCR6769399.1"
                     /translation="MLQLNENKQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTAS
                     FEDYLTLHVRDMAMNQAKIIASNDSVISAVKTRDYKRLATIANKLQRDTDFDYVVIGD
                     RHSIRLYHLNPEKIGYPMQFTKQGALEKGESYFITGKGAMGMAMRAKTPIFDDDGKVI
                     GVVSIGYLVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIA
                     RVVRQQEALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKPIAEVVRPA
                     DFFTEQIDEKRQDVVANFNGLSVIANREAIRSGDDLLGAIISFRSKDEISTLNAQLTQ
                     IKQYVESLRTLRHEHHNWMSTLNGLLQMKEYDRVLAMVQGESQAQQQLIDSLREAFAD
                     RQVAGLLFGKVQRARELGLKMIIVPGSQLSQLPPGLDSTEFAAIVGNLLDNAFEASLR
                     SDEGNKIVELFLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTRADEPGEHGIGLY
                     LIASYVTRCGGVITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
     gene            4086..>4246
                     /locus_tag="OO544_003915"
     CDS             4086..>4246
                     /locus_tag="OO544_003915"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308684.2"
                     /note="activates citrate lyase by acetylation of the gamma
                     subunit; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HCR6769400.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGG"
BASE COUNT         1048 a         1079 c          980 g         1139 t
ORIGIN      
        1 gggttgtggt tattgtgggt gtagctcgct acatcattaa agggtattcc gccactggtg
       61 tgttatttgt cggtggcctg ttattgctga ttatcagtgc cattatgggg cacaaagtgt
      121 taccgtccag ccaggcttca acaggctaca gcgccacgga tatcgttgaa tacgttaaaa
      181 tattactaat gagccgcggc ggcgacctcg gcatgatgat tatgatgctg tgtggatttg
      241 ccgcttacat gacccatatc ggcgcgaatg atatggtggt caagctggcg tcaaaaccat
      301 tgcagtatat taactcccct tacctgctga tgattgccgc ctattttgtc gcctgtctga
      361 tgtctctggc cgtctcttcc gcaaccggtc tgggtgtttt gctgatggca accctatttc
      421 cggtgatggt aaacgttggt atcagtcgtg gcgcagctgc tgccatttgt gcctccccgg
      481 cggcgattat tctcgcaccg acttcagggg atgtggtgct ggcggcgcaa gcttccgaag
      541 tgtcgctgat tgacttcgcc ttcaaaacga cgctgcctat ctcaattgct gcaattatcg
      601 gcatggcgat cgcccacttc ttctggcaac gttatctgga taaaaaagag cacatctctc
      661 atgaaatgtt agatgtcagt gaaatcacca ccactgctcc tgcgttttat gccattttgc
      721 cgttcacgcc gatcatcggt gtactgattt ttgacggtaa atggggtccg caattacaca
      781 tcatcactat tctggtgatt tgtatgctga ttgcctccat tctggagttc ctccgcagct
      841 ttaataccca gaaagttttc tctggtctgg aagtggctta tcgcgggatg gcagatgcgt
      901 ttgctaacgt ggtgatgctg ctggttgccg ctggggtatt cgctcagggg cttagcacca
      961 tcggctttat tcaaagtctg atttctatcg ctacctcgtt tggttcggcg agtatcatcc
     1021 tgatgctggt attggtgatt ctgacaatgc tggcggcagt cacgaccggt tcaggcaatg
     1081 cgccgtttta tgcgtttgtt gagatgatcc cgaaactggc gcactcttcc ggcattaacc
     1141 cggcgtattt gactatcccg atgctgcagg cgtcaaacct gggtcgtacc ctatcacccg
     1201 tttctggcgt agtcgttgcg gttgccggga tggcgaaaat ctcaccattt gaagtcgtaa
     1261 aacgcacctc ggtgccggtg cttgttggtt tggtgattgt tatcgttgct acagagctga
     1321 tggtgccagg aacggcagca gcggtcacag gcaagtaaat agtaatgccg gaggcaagtt
     1381 tcctccggct ttatcatgtc acccactgtg gtatatgcgt tgtggtctgc caactttgcc
     1441 gtgaacaatt tcagcaataa tcagatggcg gctggcgcaa tattcaagat aacgcctggc
     1501 agtggtgcag ctgatggtca gtgcctgcgc caccgtttct gccgtatgtt gcacaccagg
     1561 ctctttaaac agttttcgca ccgcgtttag cgtcaagggg tcaatgccgg tcggtagctc
     1621 gtccttaggt tcaccgcgag cataagcatt aaacatctca tcaatttgct tctggctggc
     1681 gctatcaata ctttccagca tatgtttacg ctggcggaaa cgggttagcg tttgccccag
     1741 ccgttcatag gcaatgggct taatgagata atcaaataca ccacaacgta cggcttcaga
     1801 caccgtttcc atatcgctgg ctgcagtggt aaacaccacg tcgccgggat aatgcgcctg
     1861 caccagttca tgcagtaaat taatccctct accgtcagga agatagttat cgagcaagat
     1921 tagccccggc ttaaaacgct cgatcatcat tcgggcctgc gccagatttc ccgccagtaa
     1981 tatctgactg aatccgggaa tgtgacgaat atattccgca tgcatctctg ccagcggcgt
     2041 ttcgtcctca acgatcaata gggttaatgg agctgtcatt aggtttcact ttcggaatat
     2101 agattgaaaa taaggtaccg cagggatcat tatcttcgag agtgataaca ccaccgcagc
     2161 gcgttacgta gctggcaatc aagtacaacc caatgccatg ttcaccgggc tcgtcagcac
     2221 gcgtactgac cccctgctca aatattttgt ctcttagaga ctctggaacg ccgcagccct
     2281 gatcggcgac ttcaatcacc acatcatcgc cttcatcgct gaggaataat tcaacgatct
     2341 tgtttccttc atcgctacgc aggctggctt cgaaggcgtt atcaagtaaa ttgccaacaa
     2401 tggcggcaaa ctcggtgcta tccagtcctg gcggcagttg cgaaagctgg ctaccgggga
     2461 cgatgatcat ttttagcccc agttcgcggg cgcgctgcac tttaccaaaa agcagccccg
     2521 ccacctggcg atcggcaaac gcctcacgca ggctgtcaat aagctgttgc tgggcctgag
     2581 actccccctg caccatcgcc agcacgcgat catactcttt catctgcaac agaccattga
     2641 gcgtcgacat ccaattgtga tgctcgtgtc gcaatgtacg aaggctctcg acgtattgtt
     2701 ttatttgcgt cagttgcgca ttgagggtgg aaatttcgtc tttactacga aagctgataa
     2761 tggcccccag caaatcatca cctgaacgaa tagcttcccg gttggcaata acgctcagac
     2821 cattaaagtt cgccaccaca tcctgacgtt tttcatcaat ctgttcggta aagaaatcgg
     2881 cgggcctgac cacttcagca atgggtttac ccaaccattg ccgtccggga gagctcagcc
     2941 ccagcatctt tcttgcgtta cgattgatgg cggtaatgta accatgcgga tccaccgcaa
     3001 tcagcccttc ataaaccgaa ctaaacagcg cctcttgctg acggaccacg cgtgcgattt
     3061 gctttggctc catgcccatc atttgtcgac ggatatgcgc ggccaggaac cacgacagca
     3121 acatcagaat ccctaacagc acgacaaaca cgcctgccat cggtaataaa aactcagccc
     3181 gccagctatc gattttactc accaggtagc caatcgacac cacgccgatg acttttccat
     3241 cgtcatcaaa gattggcgtt ttggcgcgca tcgccatccc cattgcccct ttcccggtaa
     3301 tgaagtagct ctcccctttc tccagcgcgc cctgtttggt gaactgcata ggataaccaa
     3361 ttttctccgg attaagatgg taaaggcgga tcgagtgccg gtcgccaatc accacataat
     3421 caaaatcggt atctctttgt aatttgttag cgatggtcgc cagccgtttg tagtcacgcg
     3481 ttttcaccgc agagatgaca ctgtcattgg aggcaataat tttcgcctga ttcattgcca
     3541 tgtcgcgtac atgaagcgtt aaatagtcct caaaactggc cgtaaaatat tgcgccaggg
     3601 ctgcaatgac aaatattgag aacaccagaa tcagcaaaaa gatacgcagc ggaaatgcca
     3661 gtctttggaa aaatgcaaac tgtttattct cgttaagctg caacattgtt ttccttgcgg
     3721 gttgtcccga gcctctttga cggatgtaat taaaatgttg aatcaaaata catagatatt
     3781 aattatgatc gtggcaaatt ttattcatta aatcaattaa atcacttaaa cctgcaggtc
     3841 atttcgtaat tacattaatt aaaaaactta aaaccattaa ataaataaaa aaccactaac
     3901 tctatgtgaa ataaatcaaa atttcacgct gcaatactcc ttagaatgta tagcgaaagg
     3961 agaaaaagat atacctcgat cacccccctt tctcccaagt gaaaataaaa ggttatcaac
     4021 ttgcaacatt gaacaacatt cgttgcaaat cgataacaac atgcaccttc aggatactat
     4081 ttattatgtt cggcaatgat attttcaccc gcgtaaaacg ttcagaaaat aaaaaaatgg
     4141 cggaaatcgc ccaattcctg catgaaaatg atttgagcgt tgacaccaca gtcgaagtat
     4201 ttattaccgt aacccgcgat gaaaagctta tcgcgtgcgg tggaat
//
LOCUS       DAJTZL010000249         4228 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.251, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000249 DAJTZL010000000
VERSION     DAJTZL010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4228
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.251"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(27..1391)
                     /locus_tag="OO544_003916"
                     /pseudo
     CDS             complement(27..1391)
                     /locus_tag="OO544_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
     gene            complement(1456..1575)
                     /locus_tag="OO544_003917"
     CDS             complement(1456..1575)
                     /locus_tag="OO544_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HCR6769401.1"
                     /translation="MPLSYVEHHHFIMMSLMSGEHNTLALSLQDEPPGLVPAG"
     gene            complement(1730..1948)
                     /locus_tag="OO544_003918"
     CDS             complement(1730..1948)
                     /locus_tag="OO544_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR6769402.1"
                     /translation="MKKLLRTLSAVDARVVCKPHRLGRSMWHLVVLLEELCKRGINFR
                     ALAQSIFAQQWGDECCKSKRICDLKVIV"
     gene            complement(2302..3780)
                     /locus_tag="OO544_003919"
     CDS             complement(2302..3780)
                     /locus_tag="OO544_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5507 domain-containing protein"
                     /protein_id="HCR6769403.1"
                     /translation="MLVSKSNEFNTSSFIDSGNCNERKSSESMELLAYSIINLICKEA
                     ASGTYRGALETLQKMMSECIYHEGNAFVIMGSGEQLKRIKYDVDENNLKVFNVYFDNN
                     EELVTDGEPDVVCLSKQVWEDLLIKLKLENKENAVSETDLSSNKNNVDQFIECAKRNE
                     QTLFDNIRKSDFHVDSFKPGSTRSVILETQPNVCMEPHNLYDNQIDKVSPVTKNSQQV
                     KGHYGDKFKEMQMFLNQMSNALQQTPSLSESKEHTIDIQKITDAFVKEFRGVLIDKNG
                     NCSERLFNFYECCYKFLPRAQPQDKIESYNSALQAFSIFRSSTLNNNDVGFNFKLFPE
                     VKLSGENLETVFKYKKGSFVREIARINITLQKEEDGLYNLGGLDFKGCFFSGQNFSNY
                     DIQYVNWRTSLFDLDTPCIFNAPAYNKSNEKSLKPVSENGLSGVLTDRNNKIKLITGV
                     APFDDILFMDDDFDDSSSEDDPVENSPVVTSPVVSSSKSSFQ"
BASE COUNT         1190 a          925 c          750 g         1363 t
ORIGIN      
        1 tgtctggaga ttcagggggc cagtctagca actaaagcac tgcccgtctc ctggttaacc
       61 ccatgtgcta taccgccggt atagacaatc agcgagcctc cggcgctaat attgctgcca
      121 gttgccgtac catctttctc aacaacctgc cggctcccgg tggaaatatt tgtcgtgtca
      181 gctttcccgc cgctcttgat attttgtgtt ccgctgttga tattggtacc cgtggcaata
      241 ccatgattat taatattctg tgtaccacca ttgattatgg tattggtcgc gtttctggta
      301 acatccatta ccccgccgtt atctattcgg gtcgcatcag ctttagcatt cgttaaaact
      361 gacattgttc ctttatcttt aataatcgtc ttatttgccg aaccatatgc gtttatgtct
      421 aaatgaccac cgttttccag caacacattg tctgccacgt gattgtggat ggagaatgca
      481 ccttcactat tcgtaccgct caccgtcgta ccgttagtgt ttgtttttaa aattgcacca
      541 tcgtgctggg taacatttgt tgccgtacca ccactaacat caagcacgcc accggaataa
      601 acttcaataa catccgagga gctggtgtaa tcaacaattt gcgtaccacc agaatagatc
      661 tgagtatttt ttgccgttga cttattattc agagactgag ttccaccttc aatcgtcgtg
      721 tccagcgcat ggctttcata tactctttgc tcaccgccat ttttaatggt ggttgttcct
      781 actgtgctct gttcaacata ctgtcgtcca ccatttatga ttgtgttcgt tgccatactt
      841 ccttgcgtga tgtcctgata accggactta tttatcgttg taccatcaac atgcccctga
      901 gttgttactg tctgccctcc accatcaatg atggttccat tcgcagtccc cccacttatg
      961 tgtgaattac cacccttaat tgtcgttcca ttgctgatac cgcctgtata aacgtcctga
     1021 ttgccactgt cgattatcgt accggtggaa atgcccccgt catgaattgt ctgtcttcct
     1081 ccgttaatgg ttgtattatt agactgcccc atataattgt tattccttcc aacatcttga
     1141 tatccaccag attcaataag acttccatta gatactccgc cataaacact ctgctggcca
     1201 tggttgataa tatgagtgtt atttgttgta cctcgttcat ctactcgttg gttgccatct
     1261 acagtctcat cgtttaccac accaataaca ttagtagtaa aggccgccat cacgggcggg
     1321 gcatatatca aggcagatat caataaggaa agtactgagc ggcgacaata atggggactg
     1381 gtcctgttca taaatttcat cctctgaaaa gtgaatactg agtagcgttt aagcgacttt
     1441 agctctgctg caacatcagc ccgcaggcac cagaccgggg ggttcatcct gaagagacag
     1501 cgcaagtgta ttgtgttcac cgctcatcaa agacatcatg atgaaatgat gatgttccac
     1561 ataagaaaga ggcatttttt aaacacagtg cgctgaagtg tgattggata aaaaagtcaa
     1621 tctattcagg aaatacgggc gtattctttt ctttcgacaa tcaggccgtc ggcaaaataa
     1681 aatgatttac ataatcgttt ctgatgaata tcttctgctc acataaaaat cacacaataa
     1741 ctttgagatc gcagattctt ttacttttac agcattcgtc cccccattgt tgggcaaata
     1801 tagattgggc cagagcacga aagttaatac cacgtttgca cagctcctcc aacagcacga
     1861 caagatgcca catactgcgc cccagtcggt gcggtttaca gactacccgt gcgtccaccg
     1921 ccgataatgt cctgagcagt tttttcagtc cggacctcct gccacggttt tacgttgaag
     1981 aggtaaccct gagcacgcag ttcttcagtc aggcgtggtg caccgtaacg ctgttattgc
     2041 tgggtaagat caaaaaactt tcaggcagct aaggaaagtt gaaccagaca ttagatgaaa
     2101 tatttcaacc aattacagca ccaatttaaa ctcagtaaat cacagcacag atcaaggggt
     2161 tacgtgaaag cgtagcccct ttttctttgg tagtggcagc aaaatggttg tagtgtaaaa
     2221 aataatcccg tttaatcaat caataataca tattgtttca atctacgtta ttatctcttt
     2281 gtaaaaatag ccatttatta atcattgaaa actgctttta gaacttgata caacgggact
     2341 agtcacaaca ggactattct caacgggatc atcctcagag gaactatcat caaagtcatc
     2401 atccataaat aaaatatcat cgaatggtgc cacgcccgtg atgagtttta ttttattatt
     2461 acgatcagtc aagactccac ttaaaccgtt ttcgctcaca ggttttaatg atttttcatt
     2521 actcttgttg taagcaggcg cattaaaaat acacggagta tcaagatcaa acaatgacgt
     2581 tctccagttc acatattgaa tatcatagtt actgaagttc tgtccagaaa agaagcatcc
     2641 cttaaaatcc aatccaccta aattatataa accatcctct tctttttgga gagtaatgtt
     2701 aattctggct atctcccgga caaaacttcc ttttttgtat ttgaataccg tttcaagatt
     2761 ttcaccagac agcttgactt ctgggaataa tttaaaatta aaccctacat cattattatt
     2821 caaagtagat gaacgaaaga tggaaaaagc ttgtagtgct gaattatagc tttcgatttt
     2881 atcttgaggc tgcgctcttg gtaaaaactt atagcagcac tcataaaaat tgaataaacg
     2941 ttctgaacaa ttcccatttt tatcaattaa taccccccgg aattctttca caaaggcatc
     3001 cgttattttt tgaatatcta tggtgtgttc ctttgactct gacaaagatg gagtctgttg
     3061 aagtgcattg ctcatctggt taaggaacat ctgcatttct ttaaatttat ctccatagtg
     3121 gcctttaact tgctgagagt ttttagttac aggtgaaacc ttatctattt ggttgtcata
     3181 taaattatgt ggttccatac agacatttgg ttgtgtttct aaaataacac tacgtgtact
     3241 acctggctta aatgaatcaa catgaaaatc actttttctt atattatcga atagggtctg
     3301 ttcatttctc ttagcgcatt caataaactg atcaacatta tttttattcg atgacaggtc
     3361 agtttcagaa accgcatttt ctttgttctc cagctttagt ttaatgagaa gatcttccca
     3421 gacctgcttg cttaaacata ctacgtcagg ctcaccatca gtaactaact cttcattatt
     3481 atcaaagtat acgttgaata cttttaagtt attttcatca acatcatatt taatacgttt
     3541 taattgctct ccagatccca taatgacaaa ggcgttgcct tcatgatata tacattcaga
     3601 catcattttt tgtaaagttt caagagcacc gcgatacgtc cctgaggcag cttccttaca
     3661 aattaaattt ataatactat aagctagtag ctccatggat tcagaagatt ttctttcatt
     3721 acaatttcca ctatctataa atgagctagt gttaaattcg ttgcttttac taactaacat
     3781 tgtctattcc tcagttaatg tctacatggc tatttttaat tttattattg tttgtcacta
     3841 taaaaaatcg ctcatttgag acaattgctg gcattaacag cttcatatgc tatacatggt
     3901 acttttaatt aaattagcac aagaatgtta aatttaataa acaaaaagtt atttcgctgt
     3961 atgataaaaa ccacccgtta taatttatta gtgaaattcg tttttcgagt gttagaaatt
     4021 tatatctaaa tagcgttgat taatgagcaa agccagactc ctgtaacgct cacaagactc
     4081 atctacctgc gacgggtatt ctggtcagta gtagatgttt aaggcgtggc agagacattt
     4141 catccttact ctacggcatt ggcccgcata caaatcgtgg ttgtgacact cacttatcat
     4201 cagtgagcga acagagaata gttcagtg
//
LOCUS       DAJTZL010000250         4200 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.252, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000250 DAJTZL010000000
VERSION     DAJTZL010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4200
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.252"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..205)
                     /locus_tag="OO544_003920"
     CDS             complement(<1..205)
                     /locus_tag="OO544_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCR6769404.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAV"
     gene            406..1140
                     /locus_tag="OO544_003921"
     CDS             406..1140
                     /locus_tag="OO544_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HCR6769405.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            complement(1142..1753)
                     /gene="emtA"
                     /locus_tag="OO544_003922"
     CDS             complement(1142..1753)
                     /gene="emtA"
                     /locus_tag="OO544_003922"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HCR6769406.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLEHVARNHPAPQAPRYIYKLEQALDAM"
     gene            1853..2767
                     /gene="ldcA"
                     /locus_tag="OO544_003923"
     CDS             1853..2767
                     /gene="ldcA"
                     /locus_tag="OO544_003923"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HCR6769407.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIQRLTDEGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAHGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCRAEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRDERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGTQLTISGHPVLKM"
     gene            2862..>4200
                     /locus_tag="OO544_003924"
     CDS             2862..>4200
                     /locus_tag="OO544_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HCR6769408.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAAL"
BASE COUNT          941 a         1091 c         1072 g         1096 t
ORIGIN      
        1 ccaccgccac caggaagctg tgcaaattaa aaccgctgat ggagccgcca atgccaaaca
       61 tggtcgccag ccagccgccg accaccgcac cgactatccc gagaatacag gtcaggaaaa
      121 atccaccacc atcacgcccc ggcatgatta gcttggcgat aatgccggct atcaggccaa
      181 aaataatcca agcaataatt cccatgttca ggccctcgtt tcgatgaatc ggacgcaaga
      241 aatgcttacg tcaacgctct aagtataggc aagggcggga tcgatgctgg gcgtatgtcg
      301 gctgatttaa gaacaacatg taaaaaatat tcacgcattt gtattaagtt ttgttaactg
      361 tgaccgataa accaaagaca gtttgtcagt caggagtttt tccgcgtgag tcattaccat
      421 gagcagttcc tgaaacaaaa tccgttagcc gtcctgggcg tgttacgcga tttgcacaaa
      481 gccgcaattc ctttgcgtct cagttggaat ggcgggcagc tgatcagcaa aatactggca
      541 ataaccccgg ataaactggt gctggatttc ggcagtcaag ccgaagacaa catcgctgtg
      601 ctaaaggcac agcacattac cattaccgcc gaaactcagg gtgcgaaagt cgagtttact
      661 gttgaacaac tacagcagag tgaatacttg cagcttccgg catttatcac cgtaccgcct
      721 cccaccttat ggtttgtaca acgacgccga tatttccgca tctccgcccc actccatccg
      781 ccttattttt gccagaccaa actggcggat aacagtacgt tacgtttccg cctgtatgat
      841 ttgtcgttag gcggcatggg cgcattactg gaaacagcaa agcctgccga attacaagaa
      901 ggcatgcgct tcgctcagat tgaagtcaac atggggcaat ggggtgtttt tcactttgac
      961 gcccagttaa tctctatcag cgagcgcaaa gtgattgatg gcaagaatga aaccatcacc
     1021 actccccgtc tgagcttccg ttttcttaac gtcagcccga cggtggagcg gcaattacag
     1081 cggattattt tctctctcga gcgagaagcc cgggaaaaag cggacaaagt gcgcgactga
     1141 tttacatcgc gtccagtgcc tgctcaagtt tgtagatata gcgcggagcc tgcggcgcag
     1201 ggtgatttcg cgctacgtgt tcgaggaact cgtcagcatc caaatcgttg attttgctga
     1261 tcgccttttt ccgatctgac gagaaagtcc gtagcagcgc acctgccccg ttagcgtatg
     1321 acaccaccag cgcatattgc agtaccttcg gatcttcaat gcctgccagc gggccggttt
     1381 ccagaatatt caggtaagcc gcccccattg aaatattacg ctccggattt ttcagctcgc
     1441 tggtcgtcgg ctcaccactc cagcccatac ggcgataaac atcacgtccg gaggttgaag
     1501 cttttaactg catcaaacca atggcattcg atttactcac cgcattcgga ttaccacccg
     1561 attcgatagc gataatcgcc gtgatcaatt gtggatcgac gccccaggct gcaccggctt
     1621 tctggcttat tggcatccac tgcatcgcac gttgcaccgg aactttcgcg ttccacggcg
     1681 ggttcgtata gtcatgcttt gatgaacaac ccgctaataa cacaatcaaa aaggcaaacc
     1741 atctcaattt cacttcttct atccttatag cctggaatcg ccgttgaggt gacaacggca
     1801 cacctaagcg gcatgatata catttcgttt ctgttgtcag caaggaattg ccatgtctct
     1861 gtttcactta attgccccat cgggttactg cattaaacag cacgccgcgc tgcgtggtat
     1921 ccagcgcctt accgacgagg ggcatcaggt caataacgtc gaggttattg cccgtcgttg
     1981 tgagcgtttt gcgggtacgg aaacggagcg cctggaagat ctcaattccc ttgccagact
     2041 gaccaccccc aacaccatcg tgctggctgt acgcggcggt tacggtgcca gtcgtttact
     2101 ggcagatatt gactggcagg cactggtggc tcgccaacaa catgacccgt tgctcatttg
     2161 cgggcatagc gattttaccg ccattcagtg cggtcttctg gcgcatggca atgtcatcac
     2221 ctttagcggc cccatgctgg tagcaaactt tggcgcggat gaactgaacg cctttaccga
     2281 gcaccatttc tggctggcgt tacgcaatga aaccttcacc attgaatggc aaggggaagg
     2341 cccgacatgt cgggccgaag gcacgttgtg gggaggcaat cttgcgatgc tgatttcact
     2401 gatcggtaca ccgtggatgc caaaaattga gaacggtatt ctggtgctgg aagatattaa
     2461 cgagcaccct ttccgggacg aacgtatgct gttgcagctc tatcatgcgg gaattttgcc
     2521 acgtcagaag gcgatcattc tcggtagctt tagcggcagc acgcccaatg attatgacgc
     2581 aggttacaac ctggagtcag tgtacgcgtt tttgcgttcc cgcctgtcga ttccactcat
     2641 taccggcctc gattttggtc atgaacaacg cacggttacg ctgccgttgg gcgcacatgc
     2701 cattctgaat aacacccggg aaggcactca gttgacgatt agcggtcatc ctgttcttaa
     2761 aatgtaaaaa aatcacgctt cagggctaag taaaacgctg tctctgccgc taatatggta
     2821 gggtttatat aagagacagc gtaatcagga gtaaccgacc gttggatgcc acaacaataa
     2881 ttagcctttt catcttagga tccatccttg tcaccagcag tatattactt agttcatttt
     2941 cttcccgtct tggcattcct attctggtta tttttctggc gatcggcatg ctggcaggag
     3001 tcgatggcgt cggcggtatc ccgtttgata attacccctt cgcctacatg gtaagtaacc
     3061 tggcactggc gattattttg ctcgacggcg ggatgcgcac tcaggccagt tcctttcgtg
     3121 tggcgttagg accggcactg tcgctggcga cgctgggcgt gcttatcacc tctggtttaa
     3181 ccggcatgat ggcggcgtgg ctgtttaatc ttgatttgat tgaaggctta ttaatcggcg
     3241 ctatcgtcgg ctctaccgat gctgcagcgg tcttttctct gctgggtggt aaggggctta
     3301 acgaacgtgt tggctcgacg ctggaaattg aatccggcag taatgatcca atggcggtct
     3361 ttctgacgat taccctgatt gcgatgatcc agcaacatga aagcagtgtc agttggatgt
     3421 tcgtggtcga tattctgcag caatttggcc tcgggattgt catcggactt ggcggtggtt
     3481 atttactgct gcaaatgatt aatcgcatcg ccctgcctgc cggattatat ccattgctgg
     3541 cattaagcgg cggtatttta atttttgctt taaccacggc gctggaaggc agcggtattc
     3601 tggctgttta tctgtgcggt tttctgctgg gtaatcggcc gattcgcaac cgctacggca
     3661 tcctgcaaaa tttcgacggc ctcgcctggc tggcgcaaat cgccatgttc ctggtgctgg
     3721 ggctattggt taacccaagc gatctgctgc ctattgccat tccggcgctc attttgtcgg
     3781 catggatgat attcttcgcc cgtccccttt cggtatttgc tggattgctc cccttccgcg
     3841 gtttcaatct gcgtgagcgc gtgtttatca gctgggtagg attacgcggc gcggtgccaa
     3901 tcatcctggc cgtgttcccg atgatggcgg ggctggagaa tgcacgtctg ttctttaatg
     3961 tcgccttctt tgtggttctg gtttcactgc tcttgcaggg aacatcactc tcgtgggcgg
     4021 cgaaaaaagc caaagtggtg gttccgcccg tcggacgtcc agtgtcacgc gttggtctgg
     4081 atattcatcc ggaaaatccg tgggagcagt ttgtttatca attgagtgcc gataaatggt
     4141 gtgtaggtgc ggcactgcgc gatctgcata tgccaaaaga gacgcgtatt gcggcactat
//
LOCUS       DAJTZL010000251         4192 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.253, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000251 DAJTZL010000000
VERSION     DAJTZL010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4192
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.253"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..215)
                     /gene="tuf"
                     /locus_tag="OO544_003925"
     CDS             complement(<1..215)
                     /gene="tuf"
                     /locus_tag="OO544_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR6769409.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDT"
     gene            complement(330..405)
                     /locus_tag="OO544_003926"
     tRNA            complement(330..405)
                     /locus_tag="OO544_003926"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(370..372),aa:Thr,seq:ggt)
     gene            complement(412..486)
                     /locus_tag="OO544_003927"
     tRNA            complement(412..486)
                     /locus_tag="OO544_003927"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(451..453),aa:Gly,seq:tcc)
     gene            complement(603..687)
                     /locus_tag="OO544_003928"
     tRNA            complement(603..687)
                     /locus_tag="OO544_003928"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(651..653),aa:Tyr,seq:gta)
     gene            complement(696..771)
                     /locus_tag="OO544_003929"
     tRNA            complement(696..771)
                     /locus_tag="OO544_003929"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(736..738),aa:Thr,seq:tgt)
     gene            1133..2083
                     /gene="coaA"
                     /locus_tag="OO544_003930"
                     /gene_synonym="panK"
     CDS             1133..2083
                     /gene="coaA"
                     /locus_tag="OO544_003930"
                     /gene_synonym="panK"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HCR6769410.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2112..3077)
                     /gene="birA"
                     /locus_tag="OO544_003931"
     CDS             complement(2112..3077)
                     /gene="birA"
                     /locus_tag="OO544_003931"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HCR6769411.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3074..4102)
                     /gene="murB"
                     /locus_tag="OO544_003932"
     CDS             complement(3074..4102)
                     /gene="murB"
                     /locus_tag="OO544_003932"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HCR6769412.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1080 a         1042 c          936 g         1134 t
ORIGIN      
        1 gtgtcgtatt caacgtgaga agtgttgatg gtgataccac gagctttttc ttccggcgcg
       61 ttatcgatct ggtcgaatgc gcgagcagca ccgccgtagg ttttagccag tacggtagtg
      121 attgcagcgg tcagcgttgt tttaccatgg tcaacgtggc cgatagtacc gacgttaacg
      181 tgcggttttg tacgttcaaa cttttcttta gacatcgatt gtccctctaa gacacggata
      241 aatcggtgat atcaccacat caaccaggca acatgcccga cttgttgaat gcaataaaca
      301 gaaggaaaaa acagggagga gaaaagaagt ggtgctgata ggcagattcg aactgccgac
      361 ctcaccctta ccaagggtgc gctctaccaa ctgagctata tcagcacatc ttggagcggg
      421 cagcgggaat cgaacccgca tcatcagctt ggaaggctga ggtaatagcc attatacgat
      481 gcccgcatcc tggaactcgg ctacctgatt ttcattctgc actgaatatc gagagaagct
      541 ctctttattc gagccggtaa gcgaacttat cgtctcgggc tacgccatcg cgtggccgaa
      601 attggtggtg ggggaaggat tcgaaccttc gaagtctgtg acggcagatt tacagtctgc
      661 tccctttggc cgctcgggaa ccccaccgga cttgatggtg ccgactaccg gaatcgaact
      721 ggtgacctac tgattacaag tcagttgctc tacctactga gctaagtcgg catcaagtag
      781 cgcgcattct atggagacat gcgagttcat gcaactaaaa aattgcataa tttgttttat
      841 tggtcacatt ttatgcgaca cgatgaagaa acagccgaac ggagggcaga ttagcacact
      901 ttttcaacat cattgtgctc aacaatgcgc tcctgctaaa ccataattct ttttatcaga
      961 tggaatatct gtcacattgc ttttcaacga tagcttcctg gcagagattt tttcttatta
     1021 ttcctcccca tctggtgtta ccctcctgcc cattaaccca ttcaacagaa ctgtgacgcg
     1081 ccatggcaaa tatcgctttg ccgatagagc tatgaccgcc agaaacatgc ttatgagtat
     1141 aaaagagcaa acgttaatga cgccttacct acagtttgac cgcaaccagt gggcagctct
     1201 gcgtgattcc gtacctatga cgttatcgga agatgagatc gcccgtctca aaggtattaa
     1261 tgaagatctc tcgttagaag aagttgccga gatctattta cctttgtcac gtttgctgaa
     1321 cttctatata agctcgaatc tgcgccgtca ggcagttctg gaacagtttc ttggtaccaa
     1381 cgggcaacgc attccttaca ttatcagtat tgctggcagt gtcgcggtgg ggaaaagtac
     1441 aaccgcccgt gtattgcagg cgctattaag ccgttggccg gaacatcgtc gtgttgaact
     1501 gatcactaca gatggcttcc ttcaccctaa tcaggttctg aaagaacgtg gtctgatgaa
     1561 gaagaaaggc ttcccggaat cgtatgatat gcatcgcctg gtgaagtttg tttccgatct
     1621 caagtccggc gtgccaaacg ttacagcccc tgtttactcg catcttattt atgatgtgat
     1681 cccggatgga gataaaactg ttgttcagcc tgatatttta attcttgaag ggttaaatgt
     1741 cttacagagc gggatggatt atccacacga tccacatcat gtatttgttt ctgattttgt
     1801 cgatttttcg atatatgttg atgcaccgga agacttactt cagacgtggt atatcaaccg
     1861 ttttctgaaa ttccgcgaag gggcttttac cgacccggat tcctattttc ataactacgc
     1921 gaaattaact aaagaagaag cgattaagac tgccatgaca ttgtggaaag agatcaactg
     1981 gctgaactta aagcaaaata ttctacctac tcgtgagcgc gccagtttaa tcctgacgaa
     2041 aagtgctaat catgcggtag aagaggtcag actacgcaaa taatttgcag gggagcgaat
     2101 actccccttt cttatttttc tgcactacgc agggatattt caccgcccat ccagggtttt
     2161 attattccat cctgctcaag taataaagcc ccctgtttgt ctattccgcg tgaaatgcca
     2221 aatatttctt tatcaccaat gataagtttc actgggcgat taataaaatt atccagcttt
     2281 tcccagcgcg acagataagg tgccaatcct tcttgttcga agagttccaa cgcagcacgt
     2341 aattcacgta ttagcatggc cgccaacgta ttacgatcga gattgatccc cgcttcctgc
     2401 agcgtgatcc acccctgatt aacgacactc tcttcaacac ggcgcattgc catgttgatc
     2461 ccggctccaa tgactatttg cgccgcatcg ccagttttgc cagtcagctc cacaagaatg
     2521 cctgccagct tgcgatcctg cagatagagg tcattaggcc atttgacacg aactttatct
     2581 gctcccagct tgcgtaatac ttccgccatc acgataccga taaccagact taaaccaatc
     2641 gccgccgccg ggccttgttc cagacgccag aacatcgaca aatataagtt tgcgccaaaa
     2701 ggcgaaaacc atttccgccc ccggcgacca cggccagcct gctggtattc tgcaacacag
     2761 gcatcgcccg atttaagctc tccgatacga tcaagaaggt actgattcgt ggagtcaata
     2821 actggcagca cggctacact accgccatcc agctgaccca atatctgttc agcattaagt
     2881 aactggatgg gctcaggcag gctgtatcct ttacccggaa cggtaaagac atcaacgccc
     2941 cagtcacgca gtgtctgaat gtgtttatta atagccgccc ggctcattcc cagcgtttca
     3001 cccaactgct cgccagagtg aaattcaccg ttcgctaaca gggcaatcaa tttcagtggc
     3061 acggtgttat ccttcatgaa attgtctcca ctgcgctcac ttcacctgat gcaccaataa
     3121 agcggacttc aggctcaagc cagacattaa atttttcacc cactttttgt cttacatggt
     3181 gtgccagttg caccacatct tcgctttttg cattgtcttc attaatgaga actaacgcct
     3241 gttgacggtg caccgcagcc ccacccattt gcatcccttt tagctggcac tgatcaataa
     3301 gccaacctgc tgccagtttt actgaaccac ccgcctgggg gtaatttggt gctgttggaa
     3361 attgcgccag taatgcttta gccgtttcgg cagatacaac agggtttttg aagaaactac
     3421 cggcattgcc attcactttt ggatcaggga gtttggtggt gcgcatatga cacaccgcat
     3481 taaatacttg ctgtggcgtt actgttgtag gatccagacg agttaagtca ccatacgtta
     3541 gtacaggttg ccactctttt ggcagacgca gacctacggc tacaatggcg aagcgatcct
     3601 ggtattcatg tttaaaaata ctgtcgcgat agccaaaacg gcactctttg gcagttaagc
     3661 gcacttgctt gcctgtcgcc agttcaacac aatcaacata agcgcaaact cgctgtaatt
     3721 ctacgccata agcaccaata ttctggatag gtgatgagcc gacacaacca ggaattaatg
     3781 ccagattttc cagaccaggc ataccttcct gcaaagtgta ttttaccagg cgatgccagt
     3841 tttctccggc tcctacatgt aaataccacg catcaggttc atcatgaatt tcgatacctt
     3901 tgatccggtt gatgatcacc gtgccacgat agtcttccag aaaaagtaca ttacttcctt
     3961 cacccagaat aagaacgggt tgtccttctg cggttgcatg ctgccaggca ttgagtaatt
     4021 gttgttcgtc ttcggcacat acaatgtgct gagcattatg atcaatgcca aatgtgttcc
     4081 agggttttaa ggagtggttc atagctgctt tcctgatgca aaaacaaggc tagtttaccg
     4141 tatctgtggg gggatggctt gtagatatga cgacaggaag agtttgtaga aa
//
LOCUS       DAJTZL010000252         4185 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.254, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000252 DAJTZL010000000
VERSION     DAJTZL010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4185
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.254"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..324
                     /locus_tag="OO544_003933"
     CDS             <1..324
                     /locus_tag="OO544_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6769413.1"
                     /translation="FYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGKATGIYYS
                     AGSIATFTIPLITAHLSQRSIADIMWFDTAIAAIGFLLALFIGLRSRKKTRHHSLKEN
                     VAPGG"
     gene            551..1816
                     /locus_tag="OO544_003934"
     CDS             551..1816
                     /locus_tag="OO544_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6769414.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMTSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFSRY
                     YRVFIIPENDVRFGERKFSTRLNTIKHRG"
     gene            1828..2694
                     /gene="ydiB"
                     /locus_tag="OO544_003935"
     CDS             1828..2694
                     /gene="ydiB"
                     /locus_tag="OO544_003935"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HCR6769415.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEVLASADILTNGTKVGMNPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            2725..3483
                     /gene="aroD"
                     /locus_tag="OO544_003936"
     CDS             2725..3483
                     /gene="aroD"
                     /locus_tag="OO544_003936"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HCR6769416.1"
                     /translation="MKTVTVKDLVIGAGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLTATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            3627..>4185
                     /locus_tag="OO544_003937"
     CDS             3627..>4185
                     /locus_tag="OO544_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6769417.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLITALADKYKQTQTPRNLSIISPTGLGDRADRGISPLAQEGLVKWALCGHWGQSPRI
                     SELAEQNKIIAYNYPQGVLTQTLRAAAAHQPGIISDIGIGTFVDPRQQGGKLNEVTKE
                     DLIKLVEFDNKEYLYYKAIAPDIAFI"
BASE COUNT         1092 a          938 c         1004 g         1151 t
ORIGIN      
        1 ttttatgtgg tgataatatt tgcttttgtc attggtttta cctctgctgg aggtgttgtg
       61 caaattggcc tgacgttaat ggctgaacgt ttcccttacg ctaaaggtaa agctacaggg
      121 atctattaca gtgcgggcag tattgcgacc tttactattc cgttgattac ggctcatctg
      181 tcccaaagaa gtattgccga tattatgtgg ttcgataccg ccatcgctgc catcggtttt
      241 ttactggcac tgtttatcgg cttacgcagc cgcaaaaaaa cgcggcatca ctcgctaaag
      301 gaaaatgtcg ctccgggtgg gtaatgcaat attcttttca ggtcatgcaa gatcttacgg
      361 ataaataact ctttctgcgc taactaagga aaatcgcgat caaaaacaaa ctatgacatg
      421 caatattcct ggaaacataa actttatgcc atgtacccag ggaaaatcat cttcagtata
      481 gtaattatgt aaaccgtcgg agaacaatac gtacggtaac gaaattatct ttcagcaagg
      541 agctgtgaaa atgtctcaaa ataaggcttt cagcacgcca tttatcctgg ctgttctttg
      601 tatttacttc agctatttcc tgcacggcat tagtgttatt acgcttgccc aaaatatgac
      661 atctctggca gaaaagtttt ccactgacaa cgcgggcatt gcctatttaa tttccggtat
      721 cggtttgggg cgattgatca gtattttatt cttcggtgtg atctccgata agtttggtcg
      781 tcgggcggtg atattaatgg cagtaataat gtatctgcta ttcttctttg gtattcccgc
      841 ttgcccgaat ttaactctcg cctacggtct ggcagtgtgc gtaggtatcg ctaactcagc
      901 gctggatacg ggtggctacc ccgcgctcat ggaatgcttt ccgaaagcct ctggttcggc
      961 ggtcatactg gttaaagcga tggtgtcatt tgggcaaatg ttctacccaa tgctggtgag
     1021 ctatatgctg ctcaataata tctggtacgg ctatgggctg attattccgg gtattctgtt
     1081 tgtactgatc acgctgatgc tgttgaaaag caaattcccc agccagttgg tggacgccag
     1141 cgtagctaat gaattaccgc aaatgaacag caaaccgtta gtctggctgg aaggtgtttc
     1201 atcggtactg ttcggtgtag ccgcattctc gaccttttat gtgattgtgg tgtggatgcc
     1261 caaatatgcg atggcttttg ctggtatgtc agaagctgag gcattaaaaa ccatctctta
     1321 ttacagtatg ggctcgttgg tctgtgtctt tatttttgcc gcactactga aaaaaatggt
     1381 ccggcccatc tgggctaatg tatttaactc tgcgctggca acaataacag ctgccattat
     1441 ctacctgtac ccttctccac tggtgtgtaa tgccggcgcc tttgttatcg gtttctcagc
     1501 agctggcggc attttacagc tcggtgtttc ggtcatgtca gagtttttcc ccaaaagcaa
     1561 agccaaagtc accagtattt atatgatgat gggtggactg gctaactttg ttattccgct
     1621 gattaccggt tatctgtcga acatcggcct gcaatatatc attgttctcg attttacttt
     1681 cgcgctgctg gccctgatta ccgcaattat tgtttttagc cgctattacc gcgtttttat
     1741 tattcctgag aatgatgtgc ggtttggcga acgcaaattt agcacccggt taaacacaat
     1801 taagcataga ggttaaagga gttaattatg gatgttaccg caaaatacga attgattggg
     1861 ttgatggctt atcctatccg ccacagttta tcgcctgaaa tgcagaataa agccttagaa
     1921 aaagcgggat tgccatttac ctatatggcc ttcgaagtgg ataacgatag ctttcccgga
     1981 gcaattgaag gattaaaagc cctcaaaatg cgcggaactg gtgtgtcgat gccgaacaaa
     2041 caactggcgt gtgaatatgt tgatgaatta acgccagcag ccaaactggt gggtgccatc
     2101 aacaccatcg ttaatgatga tggctatctg cgtggctata acaccgacgg cacgggccat
     2161 attcgcgcca ttaaagagag cggttttgat atcaaaggca aaacgatggt gctgttaggg
     2221 gccggaggtg cctcaacagc gattggcgcg cagggggcaa ttgaaggttt aaaagaaatt
     2281 aaactcttta accgtcggga tgagttcttc gataaagccc tcgccttcgc gcagcgggtt
     2341 aacgaaaaca ccgattgtgt cgtcacggtt accgatctcg ccgatcagca agcctttgct
     2401 gaagtcctgg cttccgccga cattttaacc aatggcacaa aagtaggtat gaaccccctt
     2461 gagaatgaat cattggttaa tgatatcagt ctgttacatc cgggactgct ggtcactgaa
     2521 tgcgtgtata acccgcatat gacgaagtta ttgcagcagg cacaacaagc aggttgcaaa
     2581 acgattgatg gatacggcat gttgttgtgg caaggggctg aacagttcac gttatggact
     2641 ggcaaagatt tccctctgga atatgttaaa caggtcatgg ggttcggtgc ctgacaggct
     2701 gaccgcgtgc agaaagggta aaaaatgaaa accgtaactg taaaagatct cgtcattggt
     2761 gcgggcgcac ctaaaatcat cgtctcgctg atggcgaaag atatcgcccg cgtgaaatcc
     2821 gaagctctcg cctatcgtga agcggacttt gatattctgg aatggcgtgt ggaccacttt
     2881 gccgacctct ccaatgtgga gtctgtcatg gcggcggcaa aaattctccg tgaaaccatg
     2941 ccagaaaaac cgctgctgtt taccttccgc agtgccaaag aaggcggcga gcaggcgatt
     3001 tccaccgagg cttatattgc tctcaatcgt gcagccatcg acagcggcct ggttgatatg
     3061 atcgatctgg agttatttac cggcgatgat caggttaaag aaaccgtcgc ctacgcccac
     3121 gcgcatgatg tgaaagttgt catgtccaac catgacttcc ataaaacgcc ggaagccgaa
     3181 gaaatcattg cccgtctgcg caaaatgcag tccttcgacg ccgatattcc taagattgcg
     3241 ctgatgccgc aaagtaccag cgatgtgctg acgttgctta ccgcgaccct ggagatgcag
     3301 gagcagtatg ccgatcgtcc aatcatcacg atgtcgatgg caaaaactgg cgtaatttct
     3361 cgtctggctg gtgaagtatt tggctcggcg gcaacttttg gtgcggtaaa aaaagcctct
     3421 gcaccagggc aaatctcggt aaatgattta cgcacggtat taactatttt acaccaggca
     3481 taagcaataa tatttcggcg ggaacaccct ccccgccgaa cttaaaaaat atatttaacc
     3541 gtatttaata aaaatatttc gtgagtctct gtgcgctaat tctccatctg gcgtagggaa
     3601 aatcacatct gaatcaggaa ttaacaatga aacctgtaaa accacctcgt attaatggac
     3661 gagtgccggt cctgtcggca caggaagcgg tgaattatat tcccgacgaa gcaacacttt
     3721 gtgtgttagg cgctggcggc ggtattctgg aagccaccac gttaattaca gctcttgctg
     3781 ataaatataa acagactcaa acgccacgta atttatcgat tattagtccg acagggcttg
     3841 gcgatcgcgc cgaccgtggt attagtcctc tggcgcaaga aggtctggtg aaatgggcat
     3901 tatgtggtca ctggggacaa tcgccgcgta tttctgaact cgcagaacaa aataaaatta
     3961 ttgcttataa ctatccacaa ggtgtactta cacaaacctt acgcgccgcc gcagcccacc
     4021 agcctggtat tattagtgat attggcatcg ggacatttgt cgatccacgc cagcaaggcg
     4081 gcaaactgaa tgaagtcacg aaagaagacc tgattaaact ggtcgagttt gataacaaag
     4141 aatatctcta ttacaaagcg attgcgccag atatcgcctt cattc
//
LOCUS       DAJTZL010000253         4131 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.256, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000253 DAJTZL010000000
VERSION     DAJTZL010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4131
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.256"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            115..528
                     /locus_tag="OO544_003938"
     CDS             115..528
                     /locus_tag="OO544_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HCR6769418.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(573..890)
                     /gene="hspQ"
                     /locus_tag="OO544_003939"
     CDS             complement(573..890)
                     /gene="hspQ"
                     /locus_tag="OO544_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HCR6769419.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(948..2138)
                     /gene="rlmI"
                     /locus_tag="OO544_003940"
                     /pseudo
     CDS             complement(948..2138)
                     /gene="rlmI"
                     /locus_tag="OO544_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
     gene            2233..2511
                     /gene="yccX"
                     /locus_tag="OO544_003941"
     CDS             2233..2511
                     /gene="yccX"
                     /locus_tag="OO544_003941"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HCR6769420.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(2508..2837)
                     /gene="tusE"
                     /locus_tag="OO544_003942"
     CDS             complement(2508..2837)
                     /gene="tusE"
                     /locus_tag="OO544_003942"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HCR6769421.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(2928..3587)
                     /gene="yccA"
                     /locus_tag="OO544_003943"
     CDS             complement(2928..3587)
                     /gene="yccA"
                     /locus_tag="OO544_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HCR6769422.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIVHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(3794..3881)
                     /locus_tag="OO544_003944"
     tRNA            complement(3794..3881)
                     /locus_tag="OO544_003944"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3845..3847),aa:Ser,seq:tga)
BASE COUNT         1101 a         1047 c         1017 g          966 t
ORIGIN      
        1 atacttacta acatatagac atatttcccg cttttgctct cattcattcg tattagctgc
       61 atggttggca tgtcggcttt gtcgtacact ttgttaaaca gtctggagga aataatgaaa
      121 gaaaccgata ttgcaggcat tttaacgtcc actcacacca ttgcgctggt gggggcaagc
      181 gacaaacccg atcgtcccag ctatcgcgtg atgaaatatc tactcgacca ggggtatcac
      241 gtcattccgg tttcgccaaa agttgccggc aaaacgctgc tgggccagca ggggtatggc
      301 acgctggcag acgttccgga aaaggtcgat atggtcgatg ttttccgtaa ttcagaagcg
      361 gcgtggggcg tggcacagga agctattgcc attggcgcga aaaccctctg gatgcaactg
      421 ggggtgatta atgagcaagc ggcagtactg gcacgggatg ccgggttgaa tgtggtgatg
      481 gatcgctgcc cggctattga gatccctcgc ctgggcctgg ccaaataaaa aatccccgga
      541 aggcaaaaac cttccgggga ttcgttcagg gatcagttac gcagacgcgg ggcctggagt
      601 tgtttgcgga tggtctgcgc cagctcatcc atggatggct gttcaggatg ttcatcctgc
      661 aattcgctac ttagctgggc ttcagccagg taggtatgaa ccggtaggcc gttatcgtcc
      721 tccatcacta cgtgatacca cggagcggcg cgaagctcgt catttaccgc cagctcatca
      781 ggcgacggtt cagagagcga ataaaccggg tcgatatcca cgaccactcc gagataacct
      841 aacagggaat ggcggacctg ctggccgata ccgaatttgc tggcaatcat agtcacctcc
      901 cgggaaatct ttacctttga tataagggta aaattccaca tttcaagtta catgacgcga
      961 caggcaaacc ctttcagata tagcccttcc ggataggtag cgatcaccgg atgatcggct
     1021 gcctgacgga actgctctat aaattgtaca tcacggccgg catcaattgc ggcatccgcg
     1081 atgattttct gaaataaatc gctggtcatc agaccggaac aggagaaagt caggagaata
     1141 ccgccttcat tcagcaactg aatcgccagc atgttgatat ctttatagcc acgacacgcg
     1201 cccatcaact ggcttttatt ctcaacaaac ttcggcgggt ccatcacgat aacgtcaaat
     1261 ttttcaccgc gatcgcgata agtacgcagc aatttaaaga catcatcacg gacaaactca
     1321 gccttgctca gatccagttt gttcagctca acgttctgcc gtgcaatatc cagcgcttcc
     1381 taggaggtat caacgctgac aacctggctg caaccgccca tcagtgccga tacggcgaaa
     1441 ccaccggtat aggagaaaca gttcagcaca cgtttatttt caacgtagcg gcgggtagcc
     1501 aggcggctgt ctcgctggtc caggtagtag cccgttttgt gtccgtgctg aatatccacc
     1561 agcagcttca tgccgtgttc ttcaatcggc agcagggcag gcggcaactc gccggtgacg
     1621 aggccctggg tcagctccat cccttctttt ttacgtaccg caacatcgct gcgatcgtaa
     1681 atcgcacatt ccgggtacag cgtttgcagg gcactaatta atgccgcgcg ctgatattct
     1741 gccccagcac tcagcagttg cagcaccaga aaattaccga aacgatcgat agtaataccc
     1801 ggcaggccat cagattctcc ggcgattaaa cgatagctgt cgaggccatc tttttgcgcc
     1861 agtcagtcac gccatttttg tgcttgttgc aaacggcggg aaaaaaaagc aatgtcgata
     1921 gactcagacg ggtcaaacgt ccagacgcgc gcccggattt gcgaagctgg cgaataagcg
     1981 ccgcgtgcta accattttcc ctgatgatca acaatatcga tggtttcacc gaggctggct
     2041 ttaccttcca tgcgggcaac ggccccggaa aagacccacg gatggcgacg aagtaatgat
     2101 ttttcgcgcc ctttggctaa cactaaacgt acactcataa tttacttttc tgtcgatgca
     2161 aagaaatggg cgtcattgtc cggagttttc catggatttg caacgcgtcc attaaggata
     2221 aggagagcag caatgtcgaa agtctgcata attgcctggg tttacgggcg agttcagggc
     2281 gtaggatttc gctacaccac acagtacgaa gcgaaaagac tgggtttaac cgggtacgcc
     2341 aaaaatcttg acgatggcag cgtggaagtg gttgcctgcg gtgaagaagg gcaggtggaa
     2401 aaattaatgc agtggttaaa gagtggcggc ccgcgttctg cgcgtgtaga acgggtgctc
     2461 agcgagccgc atcatccctc gggggaatta accgattttc gtatccgtta aatacatttt
     2521 accggttttg gcaggccagc aattttggtg gcttgcttag ccggaccttt cggaaacagt
     2581 cggtataaat agcggctgtt acctttttct tcgccaaatt tattcgccat cgcttttacc
     2641 agcatacgaa tcgccggaga agtattgaat tccagataga aatcacgcac aaaacgcacc
     2701 acttcccagt gttccggtga cagcgaaatc ccttcgttct ctgcaatcac cactgccagc
     2761 ggctcactcc actggctgct ttctttgaga tagccttcgg tatccgtttc tatctcttta
     2821 ccttcgaaga tcagcataat tattactacc ttaatcagac tggcggcagt gtaacaaaaa
     2881 acaaagcccc gcataaagcg aggctatgaa agtgttagcg ggtgagatta atcgcggctg
     2941 gcgaagccca gaatgctcag caggctgacg aagatgttgt acagcgaaac atacaggcta
     3001 accgtggcac gaatatagtt cgtttcaccg ccatgaacga tgttgctggt ttcaaacaaa
     3061 atagcgccag aggagatcag aatgaagacc gcgctgatcg ccagatgcag agcaggcagc
     3121 tgcaggaaga tattagcaac cataccaatc agcaccacca caatacccgc catcagcata
     3181 ccgccgagga acgacatatc tttgcgggtg gtcagcacat atgcagagca gcagaagaac
     3241 actaacgccg ttccgcccag tgccatagcg attacgtcac ccattccggc agacagatag
     3301 gtgttcagaa taggtccgag gatataaccc agaaaaccgg taaaggcgaa tgcggagata
     3361 atcccggtcg gcttattcgc cgttttatag gtcaggaaca tcaaaccata catacccacc
     3421 agcgtcagaa tcagacccgg agatggcagc atcagcaccg tactggcagt tgcggtaatc
     3481 gccgaaaagg ccagcgtcag gctcagcaga aaataggtat tacgcagcac cttatgggtg
     3541 ctaagcagtg atgtacggtc atgtgaagaa ctaacaatac gatccattga gtcactctct
     3601 atgacagatg taattaatta agcagcataa tgataatgcg taagggcacc cagaagtttt
     3661 acccatcttt acgcatttga tctggaacag gtttaacagc ggattatcag gtcattaagc
     3721 aaatataacg ccctgagaat ttcgacaggc aaaagaaaaa ggggttagca tttagctaac
     3781 cccttatctt atttggcgga agcgcagaga ttcgaactct ggaacccttt cgggtcgccg
     3841 gttttcaaga ccggtgcctt caaccgctcg gccacacttc cggaatgacg cgcactataa
     3901 acatcccgat gcggcgtgta aacccctaat ttgtttgttt gcctgaaaaa cagccaaaag
     3961 tgcattgata gcgtgaaata acagcagatt gatcatttca tcaccatgaa ttccttctct
     4021 tttactcgtt tagcaaccgg ctaaacatcc ccaccgcccg gcaaaaagaa aaataggtcc
     4081 atttttatcg ctaaaagata aatccacaca gtttgtattg ttttgtgcaa a
//
LOCUS       DAJTZL010000254         4089 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.257, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000254 DAJTZL010000000
VERSION     DAJTZL010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4089
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.257"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..879
                     /gene="adeQ"
                     /locus_tag="OO544_003945"
                     /gene_synonym="yicO"
     CDS             <1..879
                     /gene="adeQ"
                     /locus_tag="OO544_003945"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HCR6769423.1"
                     /translation="IGLFIALMGLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFII
                     TVLSSRHFHAAVLVSIVVTSCCGLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLEL
                     AGIIFSFMLINLFDSSGTLIGVTDKAGLIDGNGKFPNMNKALYVDSVSSVAGAFIGTS
                     SVTAYIESTSGVAVGGRTGLTAVVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLM
                     TSSLARVNWDDFTESVPAFITTVMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLC
                     VVVVASLFALKIILVD"
     gene            932..1384
                     /locus_tag="OO544_003946"
     CDS             932..1384
                     /locus_tag="OO544_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HCR6769424.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LSAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            1595..2775
                     /gene="nepI"
                     /locus_tag="OO544_003947"
                     /pseudo
     CDS             1595..2775
                     /gene="nepI"
                     /locus_tag="OO544_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
     gene            complement(2816..3109)
                     /locus_tag="OO544_003948"
     CDS             complement(2816..3109)
                     /locus_tag="OO544_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6769425.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            3331..>4089
                     /gene="nlpA"
                     /locus_tag="OO544_003949"
     CDS             3331..>4089
                     /gene="nlpA"
                     /locus_tag="OO544_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HCR6769426.1"
                     /translation="MKLTTHHLRTGAALLLAGILLAGCDQSSSDAKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVALGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVHDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQS"
BASE COUNT          901 a          907 c         1076 g         1205 t
ORIGIN      
        1 attggattat ttatcgcctt aatgggatta aaaaatactg gcgttattgt cgccaataaa
       61 gacacgctgg tgatgattgg cgatttaagt tctcacggcg tgttgttagg tattttaggg
      121 ttttttatta taaccgtgtt gtcatcacgt cattttcatg ccgcggtgct ggtttctatt
      181 gtggtgacgt cttgctgtgg attatttttc ggtgatgttc attttagcgg cgtctattcc
      241 attccgcctg atattagcgg cgtcattggt gaagtagatt tgagcggcgc gttaacactt
      301 gaactcgccg gtatcatttt ctcctttatg ctgatcaacc tatttgattc atcaggaaca
      361 ttaattggtg taactgataa agcgggctta atagatggta acggtaaatt ccccaatatg
      421 aataaggcgc tgtatgttga tagcgtcagt tcggtggcgg gtgcgtttat cggcacctcg
      481 tctgttactg cctatattga aagtacttct ggtgtggcag tcggtggccg cacggggctg
      541 actgcggttg tggttggcgt tatgttcctg ttggttatgt tcttctcccc gctggtggcg
      601 atggttcctc cttacgcaac cgccggagcg ttaatctttg ttggcgtgct gatgacttcg
      661 agcctggcgc gcgttaactg ggatgatttt accgaatcgg tgcctgcgtt tattaccacg
      721 gtgatgatgc cctttacttt ctcgatcacc gaagggattg cactcggctt tatgtcgtac
      781 tgcatcatga aagtgtgcac cgggcgctgg cgcgatctga acctgtgtgt ggtggtggtc
      841 gcatctctgt ttgcactgaa gattattctg gtggattagt aactggcgag ggtttccaga
      901 tatcatgagt tctgataacg caggagaact catgatctgg ataatgctcg ccacgctggc
      961 ggtagtgttt gtggttggtt ttcgggtgct gacatccggg gccagaaaag cgattcgccg
     1021 tctcagtgat cggctgaata tcgatgtcgt acccgtggag tcgatggtcg atcaaatggg
     1081 aaagtcagcc ggtgacgaat ttttacgtta tttgcatcgc ccggatgagt cgcacctgca
     1141 aaacgccgcg caggtgttgc tcatctggca aattgtcatt gtcgatggta gcgaacagaa
     1201 cctgctgcaa tggcatcgga ttttacaaaa agcccgcctt tccgcgccga ttaccgatgc
     1261 tcaggtcagg ctggcgctgg gttttctgcg cgaaaccgaa cctgaaatgc aggatattaa
     1321 tgcttttcag atgcgctata acgcgttctt tcagcctgcc gagggcgttc actggctgca
     1381 ttaaaaccgc ttcgtcttat cagcctacag accgcgcctg acctgtcggg tggataagac
     1441 gttcacgccg catccggtaa tctttgccct gttccgtttc gatcttaact ccagctcaag
     1501 caacaccgta aaagttgcaa aaacgttaaa tgcgtcacac atttcaatgt taaagtggtc
     1561 ggcttttccc ctgaaacatg ccacgggtaa caccatgagt gaatttattg ccgaaaaccg
     1621 cggcgcggat gccatcaccc gaccgaactg gtcagccgtt ttctcggtgg cgttttgtgt
     1681 cgcctgtctg attatcgttg agtttttgcc cgtcagtttg ttgacgccaa tggcccagga
     1741 tttaggcatt tcggaagggg ttgccgggca atcggtgacc gtgaccgcct ttgtggcaat
     1801 gtttgccagt ttgtttatta cccagacaat tcaggctact gaccgccgca acgttgttat
     1861 tttgtttgcc gttttgctga cgctctcctg cttgctggtt tcctttgcta actcattcag
     1921 tttgctttta atcggtcgtg cctgtctggg gctggcgctg ggcgggttct gggcgatgtc
     1981 ggcgtcgctg accatgcgtc tggtgccgcc gcgtacggtg ccgaaggcgc tgtcggtgat
     2041 cttcggcgcg gtttctattg cgctggtgat tgccgcgccg ttgggcagtt ttttaggcga
     2101 gcttatcggt tggcgcaatg tctttaatgc ggcggcggtg atgggcgtgc tgtgtatttt
     2161 ctggattatc aaatcattgc cttcactgcc aggcgaaccc tcgcatcaga aacaaaatac
     2221 tttccgctta ttacaacgtc cgggtgtgat ggcagggatg atcgccatct tcatgtcttt
     2281 cgccgggcag tttgctttct tcacatatat tcgcccggtg tatatgaacc tggcgggatt
     2341 cagcgtggat ggcttaacgc tggtgctgtt gagttttggc attgccagct ttatcggtac
     2401 gtcgctttcg tcattcattc ttaaacgttc ggtaaaactg gccttagcag gcgcgccgtt
     2461 aatactggct gtgagtgcgt tggtgctgac gttgtgggga agcgataaaa tcgttgctac
     2521 cggcgtggcg attatctggg ggctaacttt tgcattggtt cccgtcggct ggtcaacgtg
     2581 gatcacccgc tcactggccg atcaggcaga aaaagccggg tctattcagg tggcggttat
     2641 tcagcttgtg gcgcggcaat cggcggttat gcgctggata atattggtct gacttcgccg
     2701 ttgatgtttt ctggcacatt gatgttgctg actgcattgt tggttactgc aaaggtgaaa
     2761 atgaagaaat cctgatatca ggtaaagaag cgttggtcag tagttgttgc tgttcttaca
     2821 tatccgggca ttctacctta cccaacgctt cccaataatt gctctgatta ttacgttcca
     2881 tcgccacgat ggcctcgcgt atatgttgtt tattgttttc atctgacaac attaactttt
     2941 cccacgcttc atctgataat gacgcctgtg gcgtgcaaat tttacgtaaa tcttgtaata
     3001 ccgtggtttt ctcttttgcg gactgcagtg agctaaaagg cgactcggcg tatacgatgc
     3061 ctggcatagc gaaaaaagtg aaaataagca gtagtgtcgt tggtttcatg gattcaacct
     3121 tttacaaatg tgtatcgaat gaagcataag tgtagtttgc tttcatcgct ctcatcatgc
     3181 tgtacgaaac gcgcttgcgt ggtcagtaag aagtgccaga ctttatattc cacttttatt
     3241 cctttttatt cttatcgata gcgtttcgtt ttttaaaccg cagcgacctt accgctatag
     3301 tcaggtaatc attaataaaa ggataaaaaa atgaaactga caacacatca tctacggaca
     3361 ggggccgcat tattgctggc cggaattctg ctggcaggtt gcgaccagag tagcagcgat
     3421 gcaaaacaca ttaaagttgg cgttataaat ggcgcagaac aagatgtcgc ggaagtcgct
     3481 aaaaaggtgg caaaagagaa atatggcctc gatgttgaac tggtaggatt tagcggttcg
     3541 ttgctaccga acgatgcgac aaatcatggc gaacttgatg cgaatgtctt ccagcatcga
     3601 ccctttcttg aacaagataa tcaggcgcat ggttataaac tggtagcgct gggaaatacg
     3661 tttgtcttcc ctatggcggg ttattccaaa aaaatcaaaa ccgtggcgca aataaaagaa
     3721 ggcgcgacag tggcgatccc caacgacccg accaaccttg gtcgcgcact tttactgttg
     3781 caaaaagaga agctaattac tctgaaagag ggaaaagggt tattacctac cgcgctggat
     3841 ataaccgata atcctcgtca tttgcagatt atggaactcg agggggcgca gctcccgcgc
     3901 gtactggatg atcccaaagt tgatgtagcg attatcagca ccacttacat tcagcagaca
     3961 gggctttctc cggtgcacga cagcgtattt attgaagata agaattcgcc gtatgtgaat
     4021 attttggtgg cacgggaaga taataagaat gcagaaaacg tgaaggaatt tctgcaatct
     4081 tatcaatca
//
LOCUS       DAJTZL010000255         4076 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.258, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000255 DAJTZL010000000
VERSION     DAJTZL010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4076
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.258"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..784
                     /locus_tag="OO544_003950"
     CDS             <1..784
                     /locus_tag="OO544_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCR6769427.1"
                     /translation="AKTRHQINLSNSTSFSKDGYSSNNTGITGIAGEHDQLNYGIYVN
                     QQQQNNDTSLGTNLSWRTPIAIIDGSYSHSKNAWQSGGSISSGLVVWPGGINITNQLS
                     DTFAILDAPGLEGAHINGQKYNRTNSKGQVVYDLIIPHRENHLVLDIANSESETELQG
                     NRQIIAPYRGAVSYVQFTTDQRKPWYIQALRPDGSPLTFGYDVLDLQENNIGVVGQGS
                     RLFIRVDEIPTGIKVALNDEQNLFCTITFQHVIDENKTYICQ"
     gene            797..1757
                     /locus_tag="OO544_003951"
                     /pseudo
     CDS             797..1757
                     /locus_tag="OO544_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
     gene            complement(1795..2694)
                     /gene="nhaR"
                     /locus_tag="OO544_003952"
     CDS             complement(1795..2694)
                     /gene="nhaR"
                     /locus_tag="OO544_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HCR6769428.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKSFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAAR"
     gene            complement(2760..3926)
                     /gene="nhaA"
                     /locus_tag="OO544_003953"
     CDS             complement(2760..3926)
                     /gene="nhaA"
                     /locus_tag="OO544_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HCR6769429.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMMMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
BASE COUNT         1225 a          953 c          861 g         1037 t
ORIGIN      
        1 tgccaaaaca cgtcaccaaa ttaacttatc gaattcgacc tcattttcca aagatggcta
       61 ttcctccaac aatactggaa ttactggcat agccggtgaa catgatcagt taaattatgg
      121 tatatatgtt aatcagcaac aacaaaataa tgatacctcg cttggtacga atttaagctg
      181 gagaactccc atcgccataa tagatggcag ctatagtcat tctaaaaacg cctggcaaag
      241 tggtggaagt attagtagtg gattagttgt ctggcccggt ggtattaata tcactaacca
      301 gttatccgat acatttgcaa ttctggatgc gcctgggtta gaaggtgcac atattaatgg
      361 acaaaaatac aatcgaacaa acagcaaagg ccaggttgtt tacgacctga taatacctca
      421 tcgtgaaaac catctggtac ttgatatagc aaacagtgaa agtgaaacag aattgcaggg
      481 caatcgtcaa attattgcac cttaccgtgg agcagtttct tatgtgcagt ttacaactga
      541 ccaacgtaag ccctggtata tacaggcact gcgtcccgat ggttcgccat taacctttgg
      601 ctatgacgta ctggatctcc aggaaaataa tattggagtc gttggccagg gtagtcgcct
      661 ttttattcgc gtagatgaaa ttcccactgg aataaaagtt gctctcaatg atgaacagaa
      721 cttattctgt actattactt ttcaacacgt tatcgatgaa aacaaaacat atatatgcca
      781 gtaaaaggag tttactatga agtggctttt attaatcacc ttgagtctat attcttttat
      841 tgtgcaaagt gctccctgtg cattgaccaa tgtcggggaa caacgtggaa catatatact
      901 caagccactg agtatgaaag gtaatctcac cgccgaaaaa attgaaaaca ccggaaatat
      961 cattgagtgt gcagacttac aagagacgac caacgccatt aaagttcatt ttttaacaca
     1021 gaacgcttta aggtttaaag tcggaagtcg tcattatatt attaatttcc cctttgaatc
     1081 tgacggtaaa aaaaatagag cttccaggaa ggtcatctta taatttagaa gaaatgctat
     1141 ctgcgataaa tttcaccatt ccatactcta tcgcatctgt atcagatagt tccaacaccg
     1201 cagatatttt tccaggaact ccttttcctt tgttgttcga aattgaagtg agttcatgta
     1261 cagggaataa tactaattgt aatagcgtcc gctttaatta taatttaaat actattttac
     1321 aggtaaattt aatgacctgt ggttttgaag atcaatccat cgacactgga cgatttgtac
     1381 tgtctcgtat caataatgaa aattatcagt ttcatcatat aagatttgac tgcatccagg
     1441 gcgagcaagg tgaattagtt tttgaaccca gtgatgttga atattacttt gaaccagtga
     1501 cgatacagga gtcaggaacc agcatattaa aaaatgagtt agaaaacagt gaggacggtg
     1561 cgggtcaagt aggctttcaa ccctccaatg atggtacgaa tgaaatccag tatggtaaaa
     1621 gcaattacta ctcttttcag catccccatg aaggaagtaa tcagattcct ctatttattc
     1681 gcccgcgtac atatggaaat aatgtctcaa gcgggcaaat aatgagccga gtcaaaatcg
     1741 tggttatgta caactaatct atctccccca ccttaacttt gggggaggtg ccgattaacg
     1801 cgccgctgga ctaaaaagcg cagaataatc cgtattgcag attcgctgta ccgccgggtg
     1861 ctgaatcatc cgctcagcaa aaatagcatg gtactcttcc atcacattct cgacgcgacc
     1921 aatttctacg acagttttat cggcataaaa gtcatatgca taaagcgttg gggcaacgaa
     1981 gattgcattg tgcatcgcac caaaagcttt catcaacgcg gcatcatcaa actcgccgag
     2041 gatttctacg tttaatccct gggagttaaa ccagttaagc aatttgcgcc ctaacattga
     2101 acgtcgccca ggaatcaaaa gtcgccgttc ttccagacaa gccgggaacg atttttctgg
     2161 tggtggattt gtacaccaga aactcacgcc acattcgcca attctcacgg agaacaggcc
     2221 ttcctgctgc gtagagtcta tcggacagtc agaaatgatc atatccagtt tatgctgact
     2281 taattgctcc agcagcattt cgtgggtgga ttcgaagcag cgaagatgaa tgggctcgcc
     2341 ttctaccact gcggcgttaa gtacgctact gaccaggcgt ttggaaagtg catcagccac
     2401 gccaacgtca aacaataaat tggattcttt gcgatagttc acaatatcca gcatttcctg
     2461 gcttaaggtg aacattttat cggcatagcg atagaccagt tctcccagct cgctgggttc
     2521 gagaccacgt cccttgcgtt taaataattt gccttgcagg cgctcttcca gcgctcgaat
     2581 ctgtccggta atggtttgtg gagttaaata aagcgcctcc gctgcgccaa ccacggaacc
     2641 ttctttatag acatgccaga agtaatacaa gtggttgtaa ttgatatgag acatgctcat
     2701 ttctctccct gataacaatg aaaagggagc cgtttatggc tccccggtaa accgtcctgt
     2761 caaactgatg gacgcaaacg aacgcgtaac cagctgtatc caattaccgc cgaagagata
     2821 gaaccgacca ggataccgag tttcgcccag ttaatcagtt ctggatctac gctaccaaag
     2881 gccaggctgg caataaagat agacatagta aaaccgatac cgcacaggat ccccaccgcc
     2941 ataatttgct gataagtcgt tccctcaggc agatgcgcca gtttcaaacg cagcgccaac
     3001 cagcagaaca gactaatccc cagcggtttg ccaatcagca agccagcgat gatccccaat
     3061 ggcagaatgg aggtcaagcc atccagcgtg acgccttgca gtgaaacgcc agcattagca
     3121 aatgcaaaca gcggtaaaat cagatacgcc acccacgggt gcaatacatg ctccagtcgc
     3181 ttcgctggag aacgcccatg cttctctttc aaaggaataa agaagccgac aattaccccc
     3241 gccagggttg cgtgaacccc cgatttcaac accgcagtcc acaacaccac gccaacaaga
     3301 atatagacgc ccgtgcggcg tacaccacac agattcaata ccgcgagtac cgcaattgct
     3361 acagccgcga cgccaagaga ggccatcgat aagtcattag tgtagaacaa tgcgatgata
     3421 atgatggccc caagatcgtc gataatagcc agagccatca aaaagatctt cagtgctaac
     3481 ggaacacgac ttcccaacag cgccagcaca ccaagtgcaa aggcaatgtc agtagccgcc
     3541 gggatcgccc acccttcgcg ggtaatcgga tcggcatagt taaaggccag atagagtaat
     3601 gccggaacaa tcatcccacc aatagcggcg ataaccggaa atgcggcctg gcgtaagctg
     3661 gctagcgatc cttgcatcag ttcacgttta acttccagac cgactaacag gaaaaatacc
     3721 gccatcagcg cgtcatttat ccataacagc atgtttttgt tgatttcgag tgaaccaacc
     3781 cggagctgaa ccggcgtctc cagaaagtcg tgataccatc cactggttgc gccgctgttg
     3841 gccatcatca tcgccaggat agcggcaatg ataagaataa tgcctcccga ggcatcactg
     3901 ctaaagaatc gatgcagatg tttcactttt tatttctctt tcaggtgaat agatcgtttt
     3961 tactatttta ccccgcacaa atcatcgtta aaatcagatt atagtgtagg aataactcgt
     4021 tttttacgag caattaaagg atttgcgcca tcagcggtga gatgagtttt tgacgg
//
LOCUS       DAJTZL010000256         4059 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.259, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000256 DAJTZL010000000
VERSION     DAJTZL010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4059
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.259"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            35..1181
                     /locus_tag="OO544_003954"
                     /pseudo
     CDS             35..1181
                     /locus_tag="OO544_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415521.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1475..2716
                     /gene="agp"
                     /locus_tag="OO544_003955"
     CDS             1475..2716
                     /gene="agp"
                     /locus_tag="OO544_003955"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HCR6769430.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGMVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRTSAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(2754..2981)
                     /locus_tag="OO544_003956"
     CDS             complement(2754..2981)
                     /locus_tag="OO544_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HCR6769431.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKADKDSLFWGEQTIERKNV"
     gene            complement(3002..3607)
                     /gene="wrbA"
                     /locus_tag="OO544_003957"
     CDS             complement(3002..3607)
                     /gene="wrbA"
                     /locus_tag="OO544_003957"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HCR6769432.1"
                     /translation="MSVKARILVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPE
                     TMPPQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWA
                     SGALYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGG
                     TPYGATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            3659..>4059
                     /locus_tag="OO544_003958"
     CDS             3659..>4059
                     /locus_tag="OO544_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR6769433.1"
                     /translation="MKLLVTYIRWSTKEQDSGDSLRRQTNLIDAFYSKHKNDYYLLPA
                     HRYVDKGKSGFHQQHKNQGSDFRRMFENVMSGAIPEGSLIVVENFDRFSRADIDTAID
                     DVRQILRKGVSILTLGDGELYDKSALTDPVK"
BASE COUNT         1145 a          865 c          954 g         1095 t
ORIGIN      
        1 cgtcattatt tattaaattt tatagacgct atatatgggt agtaatatac atggaattag
       61 ttgcactgca aataattatt tgaaacaggc ctggaacgat ataaaaaatg agtacgaaaa
      121 aaatcaaaca tattcaatca cgctttttga aaacacactg gtgtgtttta tgcggttata
      181 caatgaactc agacgtaaag taaatgaaga ggatactcca tgtctggaat gtgaatcact
      241 agaaaaagaa tttgaggaaa tgcagaatga taatgatcta tcattattta tgagaacatt
      301 gcgtactaat gatacacaaa tttattcagg ggtttcagga ggtattacat atactataca
      361 atatgttcaa aatgttgata ttgttagagt gtccttgccg ggcagaggtt cagagtctat
      421 cacagatttt aaaggttatt attggtatgg ctttatggaa tatattgaaa acattactgc
      481 gtgtgatgat attttttctg agtattgttt agatgatgaa aatatgagta tccagccaga
      541 acagataaat acgccgggaa tatctgattt agatactggt tttaaaagaa catgagtatt
      601 catgggcgct gggcggccat aagacgcaaa agcaagtata cgaagagttt attaaaattg
      661 ttgataaaca aacgatcgag gctcttgttg atacggcatt ctataatctc agggaagatg
      721 taaagacgtt atttggcgtt aatctacaat ctgacaacaa aattcagggg cagggtggtt
      781 ttttatcatg gagttttttg tcttttaaaa aagaatttat tgatagttgc ttgaatgaca
      841 aaaaatgcat actgcattta cccgaattta tatttaatga taacaaggca cgccttgttt
      901 tagacaccga cccagagcag aaggttaatg aggtgaaaaa tttcctcact gctctttcag
      961 atagcatttg ctcattattt attgtttaca gcaatgtggc atcaatctcc ttagggaatg
     1021 aatccttttc aacagatgac gaccttgagt atgggtattt aataaacact ggcaatcatt
     1081 atgacgttta cctccctcct gaactttttg ctcaggctta cgagttaaac aataaggaaa
     1141 ggaatgcgca aattgatttt ttaactcgtt atgcaattta atgacaaagg catgtgctaa
     1201 aaaccattgt tattagcctc acttctctta ttattggtac tattgcctct gtattagtaa
     1261 cgccgctgat attctgttta tccacaggtg caattatcag cggcgtacgc gaggcagggg
     1321 ctaatcaggc atagtttgcg tcaaaccttg cctgtttttg aagatgtata tagaaaaaca
     1381 ggcgttcaac aagccatttt gcgaacctgt tcccggaaaa aagtcatatt tctgtcacac
     1441 tctttaatga ttgataacaa aagaggtgcc aggaatgaac aaaacgctaa tcgccgcaac
     1501 tgtggcaggg atagttttac tcgcttcaaa tgctcaggca caaaccgtac cggaaggcta
     1561 tcagctacag caagtgctca tgatgagccg ccataactta cgtgcgccgc tggcgaacaa
     1621 tggcagtgtg ctggagcagt cgacgccgaa taaatggcca gaatgggacg tccccggtgg
     1681 gcaactcacc accaaaggtg gcgtgctcga agtgtatatg ggccattaca tgcgtgaatg
     1741 gctggcagag caggggatgg tgaaatcggg ggaatgcccg ccgccggaca ccgtttatgc
     1801 ctatgccaat agtctgcaac gtaccgttgc gaccgcacag ttctttatta ccggcgcatt
     1861 cccggggtgt gatattcctg tgcatcacca ggaaaaaatg ggcactatgg acccaacctt
     1921 taacccggtg atcaccgatg attccgccgc attcagtgaa caggcggtgg cggcaatgga
     1981 gaaagagctc agcaaactcc agcttaccga cagctaccag ctactggaaa aaatcgttaa
     2041 ctataaagat tcccctgcct gtaaagagaa acaacagtgt tcgctggtgg atggcaaaaa
     2101 tacctttagc gccaagtatc aacaagaacc aggtgtttcc gggccgctga aagtcggcaa
     2161 ctcgctggta gatgcgttta ctttgcaata ttacgaaggt tttccgatgg atcaggtggc
     2221 ctggggagaa atcaaatctg accagcagtg gaaggtgttg tcgaagctta aaaacggcta
     2281 ccaggacagc ctgtttacct caccggaagt ggcgcgcaat gttgcgaaac cgctggtcag
     2341 ttatatcgac aaagctctgg tcaccgatcg caccagcgca ccgaaaatta cagtgttggt
     2401 tgggcacgac tccaacattg cctctctgtt aacggcgctg gatttcaaac cgtatcagtt
     2461 gcatgaccag aacgaacgca cgccgattgg cggcaaaatc gttttccagc gttggcatga
     2521 cagcaaagcc aatcgcgatt tgatgaaaat tgaatatgtg tatcagagtg cggaacagtt
     2581 acgtaatgcc gatgcgttaa ccctgcaggc acctgctcag cgtgtgacgc tggaattaag
     2641 cggttgcccg atagatgcca acggtttctg cccgatggat aaatttgata gcgtgttgaa
     2701 tgaagcggtg aaataacaga aaactccccc gcgagaagcg ggggagtcgc tggttaaacg
     2761 tttttacgtt cgatggtctg ttcgccccaa aaaagcgaat ctttatcggc cttagcaaag
     2821 gctttgacta acacttcatc gctaccttct tcccaaatct tttctgccat tttttcgtcg
     2881 tacccggcga cttcgaaaat ggcctcggct atttccggcg acgtattgcg cagagatgcc
     2941 cattcaccga cgtgatgagc tttcgcttct tgagttggca tgcgtatcct cctgttgaag
     3001 attagccgtt aagtttaact gccagacctg cgacatattc cccttgataa cgagcaatag
     3061 acagttcttc ctggcttggc tggcgtgagc cgtcaccgcc tgcgatggtg gttgcgccgt
     3121 acggcgtacc gccgcgaacc tgtgaaacgt caaataattc ctgcgctgcg tagccaatgg
     3181 ggacaatcac catgccgtga tgcgcaaggg tcgtccaggt tgaagtaata gtttgttcct
     3241 gaccgccgcc agtaccggtg gaactaaaga cgctcgccag ttttccgtat agtgcgccgg
     3301 aagcccacag gccgcccgtc tggtcgagga aggtacgcat ttgaccggac atgttgccaa
     3361 agcgggtagg tgtaccaaaa ataatggcgt cgtaatcggc cagttcttgc ggggttgcaa
     3421 ccggtgcagt ttgcgtttta ccgcctgctt tttcaaataa ttgcggcggc atggtttccg
     3481 gtacacgctt aacgacaact tctgcgccat cgactttgct tgcaccctca gcgactgcgc
     3541 gtgccatcgt ttcaatatgt ccgtacatgg aataataaag cactagtatt cttgccttaa
     3601 cactcatatc attgttctcc tgttcaatga accacttaga taagcatagt ccgaagatat
     3661 gaagctgcta gtgacataca tcaggtggag tactaaagag caagactcag gagactcctt
     3721 aagacggcaa acaaacctca ttgatgcttt ctactctaag cacaaaaacg attattacct
     3781 tcttcctgct cacagatatg ttgataaggg taaatctggt tttcaccagc aacataagaa
     3841 tcaaggttct gacttcagac gtatgtttga aaatgttatg tctggggcta ttccagaagg
     3901 ttccttgatt gttgtagaga actttgaccg cttcagtagg gctgatatcg acacagctat
     3961 agatgatgtc aggcagatac tgaggaaggg ggtctctata ctgactctgg gtgatggtga
     4021 actctacgac aagtcagcct taacagaccc tgtgaagtt
//
LOCUS       DAJTZL010000257         4046 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.260, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000257 DAJTZL010000000
VERSION     DAJTZL010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4046
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.260"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            334..1211
                     /locus_tag="OO544_003959"
                     /pseudo
     CDS             334..1211
                     /locus_tag="OO544_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000049841.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            1211..1501
                     /locus_tag="OO544_003960"
     CDS             1211..1501
                     /locus_tag="OO544_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000666098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6769434.1"
                     /translation="MKEYHGEKRYKDYLLKRYSISREGHLMKDTHGEVYRIRPKKEGR
                     GYFFYDGVTDLKIDALRFAVLYHFDIWNSIHQLRLKDGDPSNLKATNIITKR"
     gene            1822..2112
                     /locus_tag="OO544_003961"
     CDS             1822..2112
                     /locus_tag="OO544_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000149937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6769435.1"
                     /translation="MRHTVTRLLPEAYSRLAAIKDKYPAMSHTALVGEILKEHIALTP
                     FDAIKGVAEGSKAIRITTGQLELLKAQSAVAGCSMIQVLSAKVHQLADKLGV"
     gene            2112..2531
                     /locus_tag="OO544_003962"
     CDS             2112..2531
                     /locus_tag="OO544_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000882665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6769436.1"
                     /translation="MLCTFDYHAFYRHRGHLVRENNGTWKDLTRNAEPFTHQQLKSRF
                     SRWKNAPADTGDFEIIHTFDYASEDDLHDAIKLIEDFNTSEDVDGDKYKSRGNRYNRD
                     DITQAINAGQSQRYITEKYGISRRQYFYIKPLINKDN"
     gene            2699..3022
                     /locus_tag="OO544_003963"
     CDS             2699..3022
                     /locus_tag="OO544_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6769437.1"
                     /translation="MNNYPMQIMVDNDTAMMVQSFIDAGIAINFDKLLKLMADNVENI
                     SDFIQSVEFNEPRMMLPITDSNMKRLVIEQTNIYSISPEQFLKGAVTILYSDNILVTD
                     SVRVH"
     gene            3452..>4046
                     /locus_tag="OO544_003964"
     CDS             3452..>4046
                     /locus_tag="OO544_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001461099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6769438.1"
                     /translation="MIRNEHEMLIEHRPSALDIHELTLRESFDRAWRESHEVNKGPYI
                     PPEPLEIPRVEIDFSMNERCELDLKLKLQRRYFQAIKDSQEAMRSVYEKIQSKLEDEI
                     DEMVISMELQTNSYAYFSRLDRDGWGYDPVEIGKVIADLPEGQRVVRTERAVRAPHRL
                     SLIVADKSQAELEEIAKEKLMKARENEINLLKSRLAKL"
BASE COUNT         1342 a          715 c          838 g         1151 t
ORIGIN      
        1 aggtgattca gatgttaaga agtatccctg tgaacaataa atgaccaatc agtaatcatt
       61 ctgtgaaata attgttgcaa tttataatcg catctgttat agtttctata tgggctgata
      121 aaaaagttca tgtcttgaag tggtaacact tggggaggtg tcagatggtt ggcgtatatt
      181 aacctctcat tcagggtata cctcagcgtt ttaatcatcg tactgtttca ggcatggact
      241 tctttatcag tccatcaatc ccccagatat tttaatcaca caggctaatc ggttttatcc
      301 ggttggcctt ttcacgtatg aggtaattta tgaatgtcta aatataaccc aaatcagttt
      361 tataaatttg atgtgcgaat ctctcaactt tcagaagaaa ttaacgcagc agcaagattg
      421 atttatatgg tcatggtcca tagctgtgac ttctatgaag agaaatacaa atcatattca
      481 ccaagtattg accatctcgc ttatatggct ggtgtatcaa agagaacagc aatcagagcc
      541 attgagacac ttgagaaaca cggtttaatt caggtgcaaa agaatcaggg gcgtcaaagc
      601 acttatcgtg tattcacact ggatgataag ccggaattac ttgtaagacc tacatcacag
      661 gaagttgcag cctcagtaag acaaaatgac accactacca gtgacaaaat gtcatggggg
      721 tagtgacaaa atgtcacccg tgttagagtt tagataagtt attagataat aatattacag
      781 ataagaataa agagactgga cttaatgatg atattaaaga agatgacttt ctttctttgg
      841 atgaattaaa tgattttctc tctatgaatg aatctgacct tgaagtaagc tctaaaaaga
      901 cttctaaaca agacagagaa atcactgatg tgagtgttga tagtgaccat aaaaacacac
      961 agtgccccgc cactgatgat gagttggacc ccttcgatga tgaagaggat gaacgtgaaa
     1021 aagccattaa gagggcagag aagaaaaaga gattcgagct tgcaagacaa aagaacaaag
     1081 tatcgggttt agacccattc gatgacagtg aagatgatta ttatctttca ccgaaaaaga
     1141 aacaaactca ggatgacaag aagcaaaaaa ttaaaccgaa gatgtatttt gccggtggag
     1201 gaattaagta atgaaagaat atcacggtga aaagagatac aaagattacc tgttaaagcg
     1261 ttacagcatt tctcgtgaag gtcatcttat gaaggatact cacggggaag tttaccggat
     1321 tagaccaaag aaagaaggga ggggttattt cttttacgat ggtgtgacag atttaaagat
     1381 tgacgctttg aggtttgcag tgctgtatca ttttgatatc tggaattcaa tccaccagct
     1441 acgattaaaa gacggtgacc ccagcaactt aaaggccacc aatattatca caaaacgtta
     1501 gtttgtttta ccatcatcaa tcgtaattgt gatttcatat ttgaaaagct taatgctgaa
     1561 attgaatcgt gtacttctgg cgataatcat ttttaaaagc tgatacacaa gaagcaccag
     1621 aaacacaaag caacctaagc ttgcgaatct gtgagattca gcaattgttt caagaaagcc
     1681 cattaattcc ccttatttat caaagggaat gacctgtatc agccccctac aaccatatta
     1741 acatgtaaat gagttgttga caatatgtta taaatatgtt aaagttagtt ataagtcaaa
     1801 gattttaaga aggaaaattt aatgagacac acagttacac gccttttgcc agaggcatat
     1861 tccagactgg cagcaatcaa agacaaatac ccagccatga gccacacagc acttgttggt
     1921 gagattttga aggaacacat agcccttaca ccgtttgacg ctatcaaggg cgttgcagag
     1981 ggttctaagg ccatcagaat cacaacgggc caactggaac tactcaaagc acagtccgca
     2041 gtggcaggtt gctcaatgat tcaggtgttg agtgccaagg ttcatcaact ggcggataag
     2101 ctgggggttt aatgctatgc acatttgatt atcatgcgtt ttatcgccat agaggtcacc
     2161 ttgtccgtga gaataacgga acatggaaag atttaactcg taatgctgaa cccttcacac
     2221 accagcaatt aaaatcccgc ttctccagat ggaagaatgc accagcagat actggtgatt
     2281 ttgagattat ccataccttc gattatgcct cagaagatga ccttcacgat gcaattaagc
     2341 tgattgaaga cttcaatacc tcagaagatg tggatggtga caaatataaa tcccgtggta
     2401 accgctataa ccgtgatgat atcactcagg ccataaatgc tggtcaatct cagcgataca
     2461 ttacagaaaa atatggtatt tcacggagac agtatttcta cattaagcca ttgattaata
     2521 aagacaatta aaaataacca tctaagaaac ggtagaatgc actttacagg cagagttgca
     2581 ccttaatttg ataaattacc ttaccagcca gtgcaactcg ctctgtaacc cgcatggtta
     2641 ctggcctcag aatggctatt ttaagaggtc tgaggtgtgt ttcataggag atttaaaaat
     2701 gaataattac ccaatgcaaa ttatggttga taacgacact gctatgatgg tgcaaagctt
     2761 cattgatgca ggtatcgcaa ttaattttga taaattacta aagctgatgg ctgataatgt
     2821 agaaaacatc tctgacttta ttcagtcggt ggagtttaac gagcctcgaa tgatgctacc
     2881 aattacagac agcaacatga aaagattggt tatcgagcaa acgaacatat acagtatttc
     2941 cccagagcaa tttttgaaag gtgctgtgac aatcctgtac agtgataata ttctggtgac
     3001 ggattctgtg agggttcact aatgggttta ataattgctt ggacgttgct tggcttattt
     3061 gttggttgta ttattaacaa tgcggtaaat aagaattgat gttatcaacc tgttaaaaag
     3121 ctgttgacaa ataaccttaa gcatggcata attttaatta tgggagagca attctctctt
     3181 atttttcttt caataatccg atggatggat attctgagtt ggaaaggtgc aatgcttttc
     3241 gtcatggcct ttcaggttag tctgagaggc tatctcaaaa agtactcttg ttcattcatt
     3301 attgaatcct ttgcccttca cagaaatgtt tagggctttt tcagttttta aaagcacact
     3361 tggcaggttc ttgttcagtc agactcttca cctctgattg ctgagtgtgc ttctataaat
     3421 tgaaataaca aataaactaa ggggtgaacg aatgattaga aatgaacatg aaatgcttat
     3481 cgaacacaga ccaagtgcac ttgatattca cgaattaact ttgcgtgaat cttttgatag
     3541 agcttggcgg gaatcacacg aagtaaataa aggtccatat attccaccag aaccactcga
     3601 gattccaaga gttgaaattg atttttctat gaacgaacgg tgtgaacttg atttgaagtt
     3661 gaagctacaa cgccgatatt tccaagctat taaagactct caagaagcaa tgagaagtgt
     3721 ttacgagaaa attcagtcca agcttgaaga cgaaattgac gaaatggtta tttcgatgga
     3781 gcttcaaacc aattcttatg cttatttctc tcgactagac cgtgacggtt gggggtatga
     3841 ccctgtagag attggtaaag tgattgcaga tttaccagaa ggtcaaagag ttgttcgtac
     3901 agaaagagct gtcagagcac cacatcgact gtctctaatt gttgctgata aatcacaagc
     3961 tgagcttgaa gaaattgcca aagagaaact gatgaaagca agagaaaatg agattaattt
     4021 gctgaaatca agactggcta agctga
//
LOCUS       DAJTZL010000258         4045 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.261, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000258 DAJTZL010000000
VERSION     DAJTZL010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4045
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.261"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            30..758
                     /gene="mpaA"
                     /locus_tag="OO544_003965"
     CDS             30..758
                     /gene="mpaA"
                     /locus_tag="OO544_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HCR6769439.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(733..1698)
                     /gene="ycjG"
                     /locus_tag="OO544_003966"
     CDS             complement(733..1698)
                     /gene="ycjG"
                     /locus_tag="OO544_003966"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HCR6769440.1"
                     /translation="MRTVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVSQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGVAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKIGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            1817..2323
                     /gene="tpx"
                     /locus_tag="OO544_003967"
     CDS             1817..2323
                     /gene="tpx"
                     /locus_tag="OO544_003967"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HCR6769441.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTDVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(2367..3908)
                     /gene="tyrR"
                     /locus_tag="OO544_003968"
     CDS             complement(2367..3908)
                     /gene="tyrR"
                     /locus_tag="OO544_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HCR6769442.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICVTQKNLVELVQKGMFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVQRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
BASE COUNT          969 a         1142 c          970 g          964 t
ORIGIN      
        1 ctgtaatact tcctgaacca gagacgatca tgaccgtaac ccgcccacgc gccgaacgcg
       61 gcgcatttcc gcccggaaca gaacattacg gacgttcatt attgggcgcg ccgttaatct
      121 ggtttcctgc ccctgcagcc agccgcgaaa gtggtttgat tctggctggc actcacggtg
      181 atgaaaactc ttcggtcgtc acgctctcct gtgctctgcg gacgttgaca ccttctttgc
      241 gccgccatca tgtggtgctg tgtgtgaatc ctgacggctg ccagctgggg ttacgggcca
      301 atgctaatgg tgtggattta aacagaaact ttccggcggc gaactggaag gaaggtgaaa
      361 cggtttatcg ctggaacagc gccgctgaag aacgtgatgt cgtattactg acaggtgaca
      421 aacctggctc agaacctgaa acccaggcgc tgtgccagct tatacaccgc attcaacctg
      481 cctgggtggt ctcattccac gatccactgg cctgtattga agatcccaga cacagcgaat
      541 taggcgaatg gctggcccag gcgtttgaat tgcctctggt aaccagcgtc ggttatgaaa
      601 cgcctggctc tttcggcagt tggtgtgcag atctcaactt gcattgcatc accgcagagt
      661 ttccgcccat ctcctccgac gaagccagcg aaaaatatct ctttgcgatg gcaaacttgc
      721 tgcgctggca tcctaaagat gcaattcgcc cgtcgtgaac tgaagcgccg gttccacatc
      781 taccgccagc caggtcggtc cgtcaagatc ggcgaaactg acctgcggca ccagcggtaa
      841 agcggcgcta atggcacgag aggtacacaa catgcagccc agcatcagac tgaaaccttg
      901 tgcacgcgct tcagtcgcca gcgccagcgc ttccgtcaga ccaccgattt tatcgagctt
      961 aatgttaacc atctcatagc gccctttcag cgccttcaga ttgctacgtg tatgacaact
     1021 ctcatcagca caaatcggca acggatgaat aaaattctcc agcgccgcat catcctgcgc
     1081 aggaaggggt tgttcaagca tcgcaacgcc taaatccgcc aatagctggc aacgcgccgc
     1141 caccccttct gcacgccagg attcatttgc atcaacgatc agcgtcgcat cgggcacagc
     1201 tgtgcgaatt gccaccatcc gctcactaat aagatggtta tccagcttca ctttcagtaa
     1261 tttcgcgcct gcctgccaga gtgttgatgc actattggcc atctgatcag gcgtaccgat
     1321 gactaccgtc tgtgcagtga taactgtctc gggaagcgtt atgccgatca aatcagccag
     1381 cgattgctgc tgttttcgcg cggccagatc ccacaatgca caatccagcg cattacgtgc
     1441 tgcgccggca gggagaattt tttgcaactc ctcccgtgtc agccctttct ctaattgcga
     1501 cacgacgctc ataatttgcg ccattaccga ggcatcactt tctccataac gcggatacgg
     1561 cgtgcattcg ccggtgcctt taataccctc ttcttccagt tcaaccacca ccacgcgcgc
     1621 ttcactgcga cttccccggg caatcacaaa cggggtatgt aagggccagg cttcctcaaa
     1681 taccttaacg gttctcatct ttactccttg ttgtcgcggt ggtctgcgga ttaagcgcaa
     1741 atagcgtttg ctgtgttatt gacagttagc ataaactagg tgtgacgtta actatatgta
     1801 aacaggaaag ataatcatgt cacaaaccgt tcatttccag ggcaacccgg ttacagtcgc
     1861 caattccatc ccgcaggcgg gtagcaaagc gcagactttt actctcgtgg caaaagatct
     1921 gtctgacgtc accctcggtc agtttgcggg taaacgcaaa gtgctgaaca ttttcccgag
     1981 tattgatacc gatgtttgcg ccgcatcagt acgcaagttt aaccaactgg caactgagat
     2041 cgacaacacc gttgtgctgt gtatctctgc cgatctgccg ttcgcccagt ctcgtttctg
     2101 cggcgcagaa ggtctgaaca acgttatcac cctctccact ttccgtaacg ctgaatttct
     2161 gcaagcttac ggtgtggcaa ttgctgatgg cccactgaaa ggtctggcag cgcgtgccgt
     2221 tgtggttatt gacgaaaatg acaatgtgat tttcagccag ctggtggatg aaatcaccac
     2281 cgagccggat tacgaagcag ctctggctgt actgaaagca taagttaata tgcctgatgg
     2341 tgttgcacca tcaggcatat tcgcgcttac tcttcgttct tcttctgact cagaccatat
     2401 tcccgcaact tattggcaat cgcggtatgt gaaacgccga gacgttttgc cagtttgcgc
     2461 gtgctgggat aattgcgata aagctgggtt aataccgagc gttcaaaacg gctggtgatt
     2521 tcgtccagcg aaccttccat cgcatcttcg cccacggcta ccgttgcggc gtcataatcc
     2581 ggcaacaaaa tatcctgtgg acgcagctca taaccgtcca gttgtgtcag tgcgcgatag
     2641 atagcgttct ttaactgccg cacatttccc ggccacgcat aacgcgtaag tacagtattc
     2701 aggtcagcgg ccagtttcgg acgctgcacg ccctgctcgt cggcaaagcg ggcgacgaac
     2761 agctcagtta acggcatgat gtcctgcgga cagtcacgta gcggcggcag attgagcgtc
     2821 aacacgttca gacgataata gagatcttca cggaacatgc ctttttgcac cagttcgacc
     2881 agattcttct gcgtaacgca aatcacccgc acatcgacat gcacctcatg gtcttcgcca
     2941 acccgacgga aagtgccatc attaaggaaa cgcagtaatt tcgcctgcat ccgtggtgac
     3001 atttccccta tttcatccaa cagcaccgaa ccaccgttcg cctgctcaaa gaatcctttc
     3061 ttcccttccg gagcatgacc aaacagttca ctctcgaccg catcttccgg tatagacgca
     3121 cagttcagcg ccaggtaagg tttgcccgct ctggggcttg cctgatggca ggcgtaggca
     3181 aagagatctt tacctgtacc tgtgtcaccc gtaatcagca gcggcgcgct tagcatcgcc
     3241 agtttctgcg cctgttcgac aacatgcttc attttcgggc tgacggcgac aatttgactg
     3301 aaggcgctga cgtcctgggc ggcgacattt tgcaactggc ggcccatacg aatcgttgat
     3361 cgcaacatca ccaccgcacc ggtcaggacg tgttgatcat tttcatcctg aagataaaca
     3421 ggcgtaatct ccatcaggaa attctgccca ttaataacga catgctcgtt atgcgaatct
     3481 tgcggttcgc tttccagcca acgtaaaaaa ttaaagccgt taatcaattg tgcggcggta
     3541 tggttgcgca ggcgatccaa tttttgccca aaaagctgac agctcgccgg gttcgccata
     3601 tccactttgc ttttcatatc gacagagagc acaggttcag gcaacgcctc cagtaacgcg
     3661 ctcaacgcca gatgctcacg ttcggaaggc atccacggga cagtacgcac atcggtaaca
     3721 cccgcaatac ggcgtatttc ggccatcaga ctgctgaaac tctcaaactc cagttcagca
     3781 aaattgaggt agattcgccc aatgggatca atctcaatac cgcgtaaatc aatgcctctt
     3841 agcacgagta gatcgagtaa ttcgcgggtc agaccgagtc ggtcttcaca aaagacttcc
     3901 agacgcatgg gaaccttcac ctgaaaaaag aacaattaat atgatgatat gacactattt
     3961 ggatagcagg aaggtttctg tcaacaatca ttgacacaaa gacggaaaaa cgggcacttc
     4021 ggcgtaaaga tatccggtca gcacc
//
LOCUS       DAJTZL010000259         4000 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.262, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000259 DAJTZL010000000
VERSION     DAJTZL010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4000
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.262"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            234..1898
                     /gene="tssM"
                     /locus_tag="OO544_003969"
                     /pseudo
     CDS             234..1898
                     /gene="tssM"
                     /locus_tag="OO544_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354198.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
     gene            1909..3261
                     /locus_tag="OO544_003970"
     CDS             1909..3261
                     /locus_tag="OO544_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001087757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ImpA family N-terminal
                     domain-containing protein"
                     /protein_id="HCR6769443.1"
                     /translation="MNSNVLTQTIVTGSDPRGLPEFSAIREEINKASHPSQPELNWKL
                     VESLALAIFKANGVDLHTATYYTLARTRTQGLAGFCEGAELLAAMVSHDWDKFWPQGG
                     PARTEMLDWFNSRTGNILRQQISFAESDLPLIYRTERALQLICDKLQQVELKRVPRVE
                     NLLYFMQHTRKRLEPQPKSNAENAAQTTVRTLIYAPETLASSTPEAVVPPLPGLPEMK
                     VEVRSLTENPPQASVIKQGSTVRGFIAGIACSVAVASALWWWQVYPVQQQLLQVNDTA
                     QGAATVWMASPELENYERRLQQLLDTSPVQPLETGMQMMRVADSRWPESLQQQQASTQ
                     WNEALKTRAQSSPQLRGWLQTRQDLHAFADLVMQREKEGLTLSYIKNVIWQAERGLGQ
                     ETPIESLLTQYHDARAQKQNTDALEKQINERLEGVLSRWLLLKNNVMPEAATGTTAEK
                     "
     gene            3285..3767
                     /locus_tag="OO544_003971"
     CDS             3285..3767
                     /locus_tag="OO544_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HCR6769444.1"
                     /translation="MANISYLSLSGETQGLISAGCSTLDSVGNKAQPEHKDQIMVYAL
                     MHSISRSQNVNHHELIITKPVDKSSPLLAKALSDNEKMAICEFILYRTSKAGIYQPYY
                     KINLSKARISSIDFVTPHAVLEKELEPQERIAFIYEDISWEHTLAGTNAMSKWQDRVQ
                     "
     gene            3811..>4000
                     /locus_tag="OO544_003972"
     CDS             3811..>4000
                     /locus_tag="OO544_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6769445.1"
                     /translation="MAGLRNNNNTQNAQWADYVGDILRGAQPINQLVPQHPYLNDVPL
                     IDELRHQNTHHVILLTLDV"
BASE COUNT         1073 a         1029 c         1070 g          828 t
ORIGIN      
        1 tggaacagac ctatctgcaa ctgctggagc aacgttacct gccctcgctg tttaacgggc
       61 tggtcaaagc gatgaacgcc gcgccgccgg agagtgaaga aaaactcgcg gtgctgcgcg
      121 tgatgcgaat gctggaggac aaaagcggac gtaacaatca ggtggtgaag cagtatatgg
      181 caaaacgctg gagcgaaaag ttccacggtc agcgcgatat ccaggcacaa ctgatgtccc
      241 atcttgacta cgcgctggct catactgact ggcacgcaga gcgtcaggcg ggcgacggtg
      301 acgccatcag ccgctggacg ccatatgaca agcccgtggt atcagcacag aaagaactga
      361 gcaaactgcc tgtctaccag cgggtttacc agagcctgaa aacgcgggcg ctgggcgttc
      421 ttcctgccga cctcaatctg cgtgaccagg tagggccaac ctttgaccag gtgtttacat
      481 ctgccgatga caacaaactg gttgttccac agtttatcac ccgctacggc ctgcaaagct
      541 attttgtaaa acagcgcgat gaactggttg aactgacagc gatggattcc tgggtactta
      601 acctcacccg caatgtgaaa tacagtgacg ccgaccgtgc ggaaatccag caccagttga
      661 ccgagcagta tatcagcgac tacaccgcca cctggcgggc tgggatggac aatctgaata
      721 tccgcaattt tgagtccatc ggacaactga ccggggcgct ggagcaggtt atcagcggcg
      781 accagccttt gcagcgggcg ctgaccgtgc tgcgtgacag cacacagcca ggcgtctttt
      841 ctgaaaaact ctctgccaaa gaacgggagg aagccctggc agagccggat taccagttac
      901 tcacccgcct cgggcatgaa ttcgccccgg aaaacagtac cctggcagta cagaaagaca
      961 aagaaagcac gatgcaggcc gtgtatcagc aactcaccga gttgcaccgc tacctgctgg
     1021 caatccagaa cgcgcctgta ccagggaaat cggcgctgaa agccgtgcag ttacggcttg
     1081 atcagaacag cagcgatccg atattcgcca cccgccagat ggcaaaaacg ctgcctgctc
     1141 cgctcaaccg ctgggttggc agactgactg actaggcctg gcatgtggtg atggtggagg
     1201 ctgttcatta tatggaagtg gactggcgcg acagcgtggt gaaaccgttt aacgagcaac
     1261 tggcaaataa ctatccgttt aatccgcgtt ctgcacagga tgcctcactg gatgccttcg
     1321 aacgcttctt taaaccggat ggcatactgg ataccttcta ccagcagaac ctgaagctgt
     1381 ttatcgataa tgacctgagt ctggaggatg gcgataacaa cgtcattatt cgcgaagata
     1441 ttattgcgca actggaaact gcgcagaaaa tccgtgacat cttcttcagc aaacagaacg
     1501 gtctgggaac atcctttgcc gtggaaacgg tatcgctttc aggcaataaa cgccgcagtg
     1561 tactgaacct tgacagtcag ttagtcgatt acagccaggg ccgtaactat accgcccatc
     1621 tggtctggcc taacaacatg cgcgaaggca acgaaagtaa gctgacgctc atcggcacca
     1681 gcggcaacgc gccgcgcagt atcagcttca gcgggccgtg ggcgcagttc cgcctgttct
     1741 gggccggaca actgaccgga gtacaggatg gcaactttac cgtgcgcttt agcgtggacg
     1801 gtggcgcgat gacctaccgt gtgcataccg acacggaaga taacccgttc agcggtgggt
     1861 tgttcagcca gtttggtctg tcagacacac tgtactgagg aggcagggat gaatagtaac
     1921 gtactgacac aaactatcgt taccggcagt gacccgcgcg ggctgccgga attcagcgcc
     1981 atccgcgagg aaataaacaa agccagccac ccgtcacagc ctgagctgaa ctggaaactg
     2041 gtggagtcgc tggcgctggc gatttttaaa gccaacggtg tggatttaca caccgccacc
     2101 tactatacgc ttgcccggac acggacacag ggactggcgg gattctgcga aggtgcggaa
     2161 ctgctggcgg caatggtaag ccacgactgg gataagttct ggccgcaggg cggcccggcg
     2221 cgtactgaaa tgctggactg gtttaactcc cgaaccggca atattctgcg tcagcaaatc
     2281 tcctttgcgg aatccgacct accactgatc taccgcacag agcgggcatt gcagcttatc
     2341 tgcgataagc tccagcaggt ggaactgaag cgcgttccgc gcgtggagaa tctgctctat
     2401 tttatgcagc acacgcgtaa acggcttgaa ccccagccga agagtaacgc tgagaacgcc
     2461 gcacagacca cggtcagaac gctgatttat gccccggaaa cactggcatc ttccacacca
     2521 gaagcggtag tgcctcccct gcccggcctg cctgagatga aagtggaagt gcgcagtctg
     2581 acagagaatc ccccacaggc cagtgtgata aagcaaggca gtacggtaag agggtttatc
     2641 gcagggatcg cctgttcagt ggctgtcgcc tcagcattgt ggtggtggca ggtctatccg
     2701 gtgcagcagc aactgttaca ggttaacgac accgctcagg gcgcagcaac ggtgtggatg
     2761 gcctcacctg aactcgaaaa ctatgagcgc aggctgcaac aacttcttga tacctccccg
     2821 gtacagccgc tggaaaccgg gatgcagatg atgcgtgttg ccgacagtcg ctggccggaa
     2881 agcctgcaac agcaacaggc ctcgacacaa tggaatgagg cactcaaaac ccgcgcacag
     2941 agtagcccgc agttgcgtgg ctggttgcag acccgccagg acttacatgc ttttgcagat
     3001 ctagtgatgc agcgcgagaa agagggacta accctttcct atatcaaaaa tgtcatctgg
     3061 caggcggagc ggggactggg gcaggaaacc cccattgagt ctctgttgac gcagtaccat
     3121 gatgcccgtg cgcagaagca gaatacagat gcgctggaaa aacaaattaa tgagcgactc
     3181 gaaggcgtgt taagccgctg gctgctgctg aagaataacg tcatgccaga ggcggcaacc
     3241 ggtaccacgg ccgaaaaata attatttaat taaaggagat tatcatggca aatataagtt
     3301 atttatcttt gagtggagag acacaagggc taatttcagc aggttgctca acgcttgatt
     3361 ctgtcggaaa caaagcgcag ccagaacata aagatcagat catggtttat gcgttaatgc
     3421 attccatctc aagaagtcaa aatgtcaatc atcatgaatt aattatcacc aaaccggttg
     3481 ataaatcctc accattgctg gcaaaagcac ttagtgacaa tgaaaaaatg gcaatatgtg
     3541 aatttatact ctaccgaacc agtaaagcgg gtatttacca gccttattat aaaataaatc
     3601 tgtcaaaagc ccgaatatca agtattgatt ttgtcacacc gcatgctgtc ctggaaaaag
     3661 aactggaacc acaggagaga attgccttta tttatgaaga tatttcctgg gagcatacgt
     3721 tagccggtac gaatgcaatg agtaaatggc aggatcgtgt acagtaactt actgtaataa
     3781 cataataaat tactattgcg gagagataaa atggctggtt taaggaataa caacaacaca
     3841 cagaatgcac agtgggctga ttatgtgggt gacattcttc ggggggctca gccgataaac
     3901 cagctagtac ctcagcaccc ttatcttaac gacgtcccac tcattgatga gttaaggcat
     3961 cagaacactc atcatgttat tctcttgaca cttgatgttg
//
LOCUS       DAJTZL010000260         3978 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.263, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000260 DAJTZL010000000
VERSION     DAJTZL010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3978
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.263"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..2839
                     /locus_tag="OO544_003973"
                     /pseudo
     CDS             1..2839
                     /locus_tag="OO544_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat protein"
     gene            3820..3978
                     /locus_tag="OO544_003974"
                     /pseudo
     CDS             3820..3978
                     /locus_tag="OO544_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          992 a          966 c         1231 g          789 t
ORIGIN      
        1 tgccgggcgg gagttccgtc tggtgctgac cacgcaggcg cagcgtgcgg aagaggcccg
       61 cacctcttcg ctatcttctt ctgacagttc ccgccctctc tcagcctcag cgttccccga
      121 cacactgccc ggtaccgaat acggccccga caggggtatc cgcctttcgg cggtgtggtt
      181 gatgcacgac ccggcatacc cggagagcct gcccgctgcg ccactggtgc ggtacacgta
      241 tacggaagcc ggtgaactgc tggcggtata tgaccgcagc aatacgcagg tgcgcgcttt
      301 cacgtatgac gcgcagcacc cgggccggat ggtggcgcac cgttacgcgg gaaggccgga
      361 gatgcgctac cgctacgacg atacggggcg ggtggtggag caactgaacc cggcagggtt
      421 aagctaccgc tatctttatg agcaggaccg catcaccgtc accgacagcc tgaaccggcg
      481 tgaggtgctg catacagaag gcggggccgg gctgaaacgg gtggtgaaaa aagaactggc
      541 ggacggcagc gtcacgcgca gcgggtatga cgcggcagga aggctcacgg cgcagacgga
      601 cgcggcggga cggcggacag agtacggtct gaatgtggtg tccggcgata tcacggacat
      661 caccacaccg gacgggcggg agacgaaatt ttactataac gacgggaacc agctgacggc
      721 ggtggtgtac ccggacgggc tggagagccg ccgggaatat gatgaaccgg gcaggctggt
      781 atcggagaca tcgcgcagcg gggagacagt acgctaccgc tacgatgacg cgcacagtga
      841 gttaccggcg acgacaacgg atgcgacggg cagcacccgg cagatgacgt ggagccgcta
      901 cgggcagttg ctggcgttca ctgactgctc gggctaccag acccgctatg aatacgaccg
      961 cttcggccag atgacggcgg ttcaccgcga ggaaggtatc agcctttacc gccactatga
     1021 caaccgtggc cggttaacct cggtgaaaga cgcacagggc cgtgaaacgc agtatgagta
     1081 caacgccgca ggcgacctga ctgccgttat caccccggac ggcaaccgga gcgagacaca
     1141 gtacgatgcg tggggaaagg cggtcagcac cacgcagggc gggctgacgc gcagtatgga
     1201 gtacgatgct gccggacgtg tcatcagtct gactaacgag aacggcagtc acagcgactt
     1261 cagctacgat gcgctggacc ggctggtaca gcagggcggc tttgacgggc ggacgcaacg
     1321 ttatcattat gacctgaccg gaaaactcac gcagagtgag gatgaggggc ttgtcaccct
     1381 ctggcactac gatgcgtcgg accgcatcac gcaccggacg gtgaacggcg acccggcaga
     1441 gcagtggcag tatgacggcc acggctggct gagagaaatc agccacctga gcgaaggcca
     1501 ccgtgtcgct gtccactacg gctacgacga taagggccgc ctgacgggcg aacggcagac
     1561 ggtggagaac ccggagacgg gggaactgct gtggcagcat tagacgaaac acgcctacaa
     1621 cgaacagggg ctggcaaacc gcgtcacgcc ggacagcctg ccgccggtgg agtggctgac
     1681 ctacggcagc ggttacctgg cgggcatgaa gctgggcggg acgccgctgg tcgagtatac
     1741 gcgggacagg ctgcaccgtg agacggtgcg caccctcgca ccggacctgg acatccgcat
     1801 cccgtatgcc accgacccgg cgggaaaccg cctgccggac ccggagctac acccggacag
     1861 cacgctcacg gcgtggccgg ataaccgcat cgcagaggat gcgcactatg tttaccacta
     1921 tgatgaatac ggcagactga cggagaagac ggaccgcatc ccgacgggtg tgatacggac
     1981 ggacgacgag cggacccacc actacgacag ccagcaccgc ctggtgttcc acacgcggat
     2041 acagcatggc gagccactgg tcgagagccg ctacctctac gacccgctgg gacggcgaat
     2101 ggcgaaacgg gtatggcggc gggagcgtga cctgacgggg tggatgtcgc tgtcgcgtaa
     2161 accggaggag acatggtacg gctgggacgg cgacaggctg acgacggtac agaccgacac
     2221 cacacgtatc cagacggtat acgagccggg aagcttcacg ccgctcatcc gggtcgagac
     2281 agagaacggc gagcgggaga aagcgcagcg ccgcagcctg gcggagaagc tgcagcagga
     2341 agggagcgag gacgggcacg gcgtggtatt tccggctgaa ctggtgcggc tgctggacag
     2401 actggaggaa gaaatccggg cagaccgcgt gagcagtgaa agccgggtgt ggcttgcgca
     2461 gtgcgggctg acggtggagc aactggccag acaggtggag ccggaataca caccagtgcg
     2521 aaacgttcat ctttaccact gcgaccatcg gggccagctg ctggcgctca tcagcgaaga
     2581 cggcgatacg gtgtggagcg gggcgtatga tgagtggggc aaccagctaa atgaggagaa
     2641 cccgcatcat gtgtatcagc cataccgtct gccggggcag cagtatgatg aggagtcggg
     2701 gctgtactac aatcgtcacc ggtactatga tccgttgcag agacggtaca tcacccagga
     2761 ccccattggg ctgaaaggtg ggtggaatct gtatcagtat ccgctgaatc cggtgagcag
     2821 gatagatccg ttggggttat tctcatgtga gagttgtagt cattttatag atgatataaa
     2881 taataaactg gataaccttc cgtttaaccc gacaaaaatg tttacagcta gtttaaatac
     2941 tgctaattca gcaaggctgt atgcaagtgg catgacaaaa ttcggtataa gtgttgctgc
     3001 cgatggaacg atcattgctg ccccagtcgg tgcagcttct atggctttag gggcgtggaa
     3061 tataaaatct gcgacagctg catataacag agcaaattta caattatcag aatcacttca
     3121 ggaaaatggt agagataggt gttggagaaa tttattgggt attttacctt atggtgagca
     3181 gtttgatgac cctggtgaac catcaatatt agacgtttat ggtgaaaaga ttgagaatat
     3241 caaagattct ccaatgaagt tttttgaaga gcttgggacg ctggggttgt aatgtgtatt
     3301 tttaatgata tttttatgtt tgtgtttatt gttagtagta tacttgtaac cgtgcatttt
     3361 gtgtattctt tattttttaa aagtggaaag gtaaaagata aggtaaaaaa atggattaaa
     3421 gtaatgtgtg atttattttg ggggatagga tagtggtgtg atatggataa atataaatta
     3481 cagtggctgg catatttcga cctgacgccg gagtagataa aaaaacagac aggattggag
     3541 tatagcaagc gtctgacaat ctctcgggac agtagtatga tgaagaggcg gggttgtact
     3601 ataaccgtaa ccggtactac gatccgctaa agggcaagta tatgactcag ggaaagatca
     3661 tcagaagttc actttttgta ctaaataatt cgcattttat gtttaaaaat tgagatattc
     3721 ctcattacct gaagctgttt ttttattgct tatacataat caaatactcc ttacataatt
     3781 aaggagaaca aaatggaaat taaaaaattg atggaacatt ttttgaagca atatggtgat
     3841 tttgaaaatg gcattcctgt tcacgatacc attgccagag ttgtatcctg tatcagtcct
     3901 gcaaaatttc atgagtgctt tattaactgg atgcgtgact gccattcttc agatgataaa
     3961 gacgtcattg caattgat
//
LOCUS       DAJTZL010000261         3972 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.264, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000261 DAJTZL010000000
VERSION     DAJTZL010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3972
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.264"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            168..503
                     /locus_tag="OO544_003975"
     CDS             168..503
                     /locus_tag="OO544_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR6769446.1"
                     /translation="MLSAFVIGAYSAYWLWNNNQPKPFFKDYKTVAEINGCHFNVTED
                     TIDGLKEFDKYKTRILDSGINCKKYPWLYFPLAKSSPGMIVMACNKNYNQHEVADCLT
                     LSYREVNRD"
     gene            496..978
                     /locus_tag="OO544_003976"
     CDS             496..978
                     /locus_tag="OO544_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6769447.1"
                     /translation="MIDYKKNLLFILVFISGFILFTVYSYTAEKMIYNETCTANWVIF
                     NDQGRANLTIDFMYNKKNKTGTVALSGTWQQGNRESKSIRRNIEYTWIENYDTAHLTS
                     KKVNKFEIMDQVDDDRLAQLIPDFYVFPEKSVSYNILKQGKHAFILSIGNRAIMHCAR
                     "
     gene            complement(1012..1092)
                     /locus_tag="OO544_003977"
     CDS             complement(1012..1092)
                     /locus_tag="OO544_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6769448.1"
                     /translation="MKDVDQNFDALDCHILREYLILLFYD"
     gene            complement(1128..1552)
                     /locus_tag="OO544_003978"
                     /pseudo
     CDS             complement(1128..1552)
                     /locus_tag="OO544_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417326.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1982..2472
                     /locus_tag="OO544_003979"
                     /pseudo
     CDS             1982..2472
                     /locus_tag="OO544_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311734.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2697..3188
                     /locus_tag="OO544_003980"
     CDS             2697..3188
                     /locus_tag="OO544_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCR6769449.1"
                     /translation="MSTETIEIFNNSDEWANQLKHALSKGENLALLHGLTPDILDRIY
                     AYAFDYHEKGNITDAEIYYKFLCIYAFENHEYLKDFASVCQPKKKYQQAYDLYKLSYN
                     YSPYDDYSVIYRMGQCQIGAKNIDNAMQCFYHIINNCEDDSVKSKAQAYIELLNDNSE
                     DNG"
     gene            3523..>3972
                     /gene="ygeH"
                     /locus_tag="OO544_003981"
     CDS             3523..>3972
                     /gene="ygeH"
                     /locus_tag="OO544_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HilA family transcriptional regulator YgeH"
                     /protein_id="HCR6769450.1"
                     /translation="MDLENKFSYHFLEGLTLTEDGILTQGNEQVYIPQKELGVLIVLL
                     ESAGHVVLKDMIIESVWKNIIVSDESLTRCIYSLRCIFEKIGYDRCIETIYRKGYRFS
                     GQVFKTKINEDNTSDYSIAIFPFTTSLNTLDPLILNQELVQIISNKKI"
BASE COUNT         1488 a          635 c          638 g         1211 t
ORIGIN      
        1 tccataatga cattaacata caaaatcatg taataaactc gtcaacagac gcacatacac
       61 acaatgcccc acctgccata aaagttaatg cgggatacaa agagagcatt ggtggcgcaa
      121 agaatttcaa taacaaaatc ctcaaacata taaaaagtca tctaattatg ttgagcgcat
      181 ttgtcatagg cgcatattct gcatattggc tatggaataa taatcaaccg aagccatttt
      241 tcaaggatta caagactgtt gcggaaatta atggctgcca ttttaatgta acagaagata
      301 caattgatgg gttgaaagag ttcgataaat ataaaacacg gatactggat tcggggatta
      361 actgtaaaaa atatccgtgg ttatacttcc ctcttgcaaa atcctcaccg gggatgattg
      421 ttatggcatg caataaaaac tataaccaac atgaagtggc agactgttta accctgtctt
      481 accgtgaggt taatcgtgat tgattataaa aaaaacttat tatttatttt agtttttatc
      541 agtgggttta ttctcttcac tgtctattct tacactgcag aaaaaatgat atataacgaa
      601 acatgcactg caaattgggt aatttttaat gaccagggac gagcaaatct taccatcgac
      661 tttatgtata acaaaaaaaa taaaacagga actgttgcgt tgagtggaac ctggcaacaa
      721 ggtaacagag aaagtaaatc aatacggcga aatattgaat acacatggat tgaaaactat
      781 gacacagccc atttaacatc aaagaaagtt aataagtttg agattatgga tcaagttgat
      841 gatgatagac ttgcacaact tattcctgat ttttatgtct ttccagaaaa aagtgtaagc
      901 tataatatac taaagcaagg taagcatgct tttattttga gcattggtaa cagagcaata
      961 atgcattgtg caaggtaaac taaaatagat aacaaaaaag atggaacctc gttaatcata
     1021 aaacaataaa attaaatatt ctcgcagtat atggcagtct aaagcatcaa agttttgatc
     1081 aacatctttc attttagaca tctccttgca atgtaaaata tcatgtatca acaacatctg
     1141 gtgcaaatcc atgagtcgaa ctcgacattc atcttagctc gattattatt ccttcgtaca
     1201 gtcgatgtaa acaatacaga aagaggatta ttaagaacca gtttactcag gaatcaatag
     1261 aggaaaggac taacgtttct ttaaaagaat tgatttcatc atctgttaaa ctaaactcat
     1321 cattgacaga tcgtgagata taactgtttt taactttact ctttacgttg actttattga
     1381 cagaattaac attcacatat cttgaattta atgtccatga tgttgtttgc gtgagaacat
     1441 tctcagcatt aaggaatttt tttaacggaa tccttggttt ctttttcgag tccgaattta
     1501 caatatcatg catatagacc atttcatgaa tctgcgaaaa ctcaatgtcc attgtatacc
     1561 tcacattttt accgtgactc gatgttactg ttcaataatc accttccatc aatactaaaa
     1621 ttaatacccc taatgtgccg ataacaaata tagtcattct acgtaacgtc tccataaggt
     1681 gataaccttg atcaaatgaa tttcgaatat aataaaggaa atcttttgag atgtttatca
     1741 cgaataaagc tatttatgtt accaatatca tttcgagtgc gcacactatc tatctgtttt
     1801 ttcgagatat aaaaaacaac gtgtaagtta tgtaaattgc cgatgctact gagtaacatt
     1861 atggtaaaaa aagtaaaaat gagcgatttt tgatagaata aagcatgaga aaaggtgata
     1921 tttgacatta tcagaagctg cgaattcgga ttttgctcta atcaaatgag agaaatgtaa
     1981 aatgaagcca cgaaatatta ataatagcct accactgcaa ccattagttc ctgatcagga
     2041 gaacaaaaat aagaaaaatg aagagaaatc cgttaatcca gttaaaatca caatggggtc
     2101 tggtttaaat tatattgaac aagaatctct tggaggaaaa tatctaacac atgatttgtc
     2161 aataaagata gcggatattt ctgaagagat aattcagcaa gcaatattat ctgctatgag
     2221 catatataaa ttttcgataa cagatgattt aatgagtatg gctgtaaatg aactcataaa
     2281 actgaccaaa atagagaata atgtagacct gaataaattc actactatat gcacagacgt
     2341 tctatccccc ctcgtcacca gacataataa agaaaaaaaa caaacgacat tctacccttc
     2401 tcaaaaatcc cctttttaat tttcattgaa aaaatggcaa ttgatactat gaatccaggt
     2461 catacacatt aaggcattta tgtaatgtcg taattaagat aactaataag gtgaatatta
     2521 gtaaaacaac taatgatgtt attagtttgt agtgaacagt acttttacca ataatgaaaa
     2581 atataccata agcaatgcaa gcttaaaact aataatttaa aaaagataaa tgtaaaaata
     2641 ttaatgagtc aatcaataat aatttagcat cacgagacac atcacagagg aatattatga
     2701 gcacagaaac aattgaaata ttcaataata gtgatgaatg ggcaaatcaa ctaaaacacg
     2761 cattatcgaa aggagaaaat ctggcattac tacatggttt aactcctgat atccttgata
     2821 gaatatatgc atatgcattc gactaccatg aaaagggtaa tataacggac gcagaaattt
     2881 attataaatt tttgtgcatt tatgcgttcg aaaatcatga gtatctaaaa gattttgcat
     2941 cagtatgtca gccaaaaaag aaatatcaac aagcatatga cctttacaaa ctaagttaca
     3001 attactcccc gtatgatgac tattcagtta tttatcgtat gggtcaatgt cagattgggg
     3061 ctaaaaatat cgataacgca atgcaatgtt tctatcacat tattaacaat tgtgaggatg
     3121 atagtgttaa gagtaaagcg caggcatata ttgaactctt aaacgataat tcagaagata
     3181 atggctaagc attacaatca tttggttaaa tttgctggtt atagtgcagt taactactat
     3241 aaccagcacc tcttatgttt tcttaaatca gcatcttaaa acagttaaga gcatactata
     3301 aatcattatt tttcaatgcg ttagagaaca atctacttta taaaaattga ataaggctat
     3361 ttcagattac atcactgaat tcctaagata aattagtgaa cacaaccata gatatgtcga
     3421 atgtattttc ctgtagcatt aatgacacat aaatatgaat agccataatt tctattgctt
     3481 aagcaggatg caagaaacca attttttcat agaggttaac taatggactt agaaaataaa
     3541 ttctcatatc attttcttga gggattaacg ctcacggaag atggaattct tactcaagga
     3601 aatgagcaag tttatattcc acagaaagag ttaggtgtat taatagtatt acttgaatcc
     3661 gctggtcatg ttgtactgaa agatatgatc atcgaatcag tatggaaaaa tattattgtt
     3721 agtgacgagt ccctgacaag atgtatctat tctttgcgct gcatttttga aaaaattggc
     3781 tatgatcgtt gcatagaaac aatctaccgg aaaggttatc gtttcagcgg gcaggttttc
     3841 aaaactaaaa ttaatgaaga taatacttca gactattcca tagctatatt ccctttcact
     3901 acttcattga atacactgga tccattaata cttaatcagg aattagtgca aatcatttca
     3961 aataaaaaaa tc
//
LOCUS       DAJTZL010000262         3961 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.265, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000262 DAJTZL010000000
VERSION     DAJTZL010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3961
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.265"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..242)
                     /locus_tag="OO544_003982"
     CDS             complement(<1..242)
                     /locus_tag="OO544_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR6769451.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSI"
     gene            complement(453..1850)
                     /gene="ccp"
                     /locus_tag="OO544_003983"
     CDS             complement(453..1850)
                     /gene="ccp"
                     /locus_tag="OO544_003983"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HCR6769452.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLGVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            2255..3904
                     /locus_tag="OO544_003984"
     CDS             2255..3904
                     /locus_tag="OO544_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HCR6769453.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
BASE COUNT         1032 a         1001 c          928 g         1000 t
ORIGIN      
        1 ttgatcgaca aatccatcaa tttatggacg ttttcgtcgt cccaggtctg gcagaaagtg
       61 gccaggttct gacgagcgtt gccatcaaga tataattcat cattgataat ctggaatgcg
      121 acatcatcgc gcatttcgtg cagcggaaat cgttttgact ccgcgatagt agaaatggcc
      181 tttgcgccaa aacgtgaatc gagtagttct gagcggaaat ccgttaacag cttctggtcc
      241 atttcgaact ccttaaattt atttgaaggc aataaaaaag taggatttat ccgcaatgga
      301 agcaaggcat tacaggctta atttaaataa caaaatccta agcagaaaaa cgacttttta
      361 taatataaac atttaacatg ataatattaa aaacagactt tattatcgta ataaaaatcg
      421 cccaaacatt cctgttcggg cgatcaaatt aattattgtt tatcctgcat atacggcgtg
      481 tacaccccgt tcagactgtg caggaaagct acgatatcat ccacatcctc ctgcggcagc
      541 tctttgccta cctgatagcg cagcatcagt ttcaccgccc cgtccagcgt cggcacgtca
      601 ccgcgatgga agtacggtgc cgttaaagca acgttacgta aaccgggtac tttctgacgc
      661 aatttatcac gctcttcttt agtaacattc atacgaccaa tatccgccgc cgtaatttcc
      721 ccaaagttaa agtctttttt cagccccaac ggttcaaagg aacgtccacc gagaataata
      781 ccaccatgac aagttgcaca tttattatct ttaaataatt gatagccttt tttctgttgc
      841 gccgtcagcg cattttcatc tccacgcaac catttatcaa atggggaatc cggcgtaatt
      901 aatgttttct caaattcagc aatggcatca gtaatatttt cgccactgaa accttgcgga
      961 tagacgccga ggaactgcgc tttaagctgc ggatcttttt ccagcttagc aataatttcg
     1021 tcccaagatt tcgacgccat ttcaatcggg ttcaacggcg gtccaccagc ctgatcctgc
     1081 aatgttgccg cacgaccatc ccagaactgc tcaacgttaa atactgagtt aaataccgtc
     1141 ggcgcgttaa tcggcccaac tgcgccacca acaccaatcg atgtttttct gccatcgacg
     1201 ccccccgcat tcaacgcatg gcaatgagcg catgaaatgg tgctatcagc cgataaacgg
     1261 ggatcgtgat acagcgcaaa gcccaacgcc actttttgcg catcggtagg cagtttttgc
     1321 gggatgggct gcaccggttc attgcgatgc tccggagcag tatcattgct ggcgtaatat
     1381 tccgcgcgct gttttgcaat ccaggccagt atttccgccc gctcttcatc actcacctta
     1441 cccgcccagt gaagcgcggt ataacgcgtt ggcggcatag tttcatactg catcacccat
     1501 tcaatcttat tcagatcgct ttgcgaaacg ggtttatcag ccagcagtgc cgcacgcacg
     1561 gcctcgaggt taaaagattt atatccaagc ttaatgtcgt aatccatcaa ctgtttcgcg
     1621 ccaggaatat aataataggc gggtaattct gccgaaggcg tgtggcaata gtcgcatcct
     1681 ttttcgcgga gaaaacctaa aaccttatta ttttcactga cagcggatgc ctgaacatcg
     1741 gcctgtttac tgcgtttatt atcgtggtac cacacataac cagataaccc taaatagcaa
     1801 atcgcgacgc cagccaggcc gatcgcggta atacgtgaga ccattttcat tattcttccc
     1861 tcacggtggt gagttattgt gacccagaaa attttccaga atgctgcaaa cgtctctgcg
     1921 agacaaagcc atcataaaaa acagtgagat acgctttttg ataggaatca atcaattaca
     1981 tgaatataaa ctatcaataa gatagcctgt gcctattacc cagatcagga atggcatatc
     2041 atgattgcgg cgagagcagg attgaatgtt ctgaaaatga aaaacaacac gaaatccgta
     2101 tttttataaa ttccatggat attagtgcgt tatatttttt gacaggttaa taaacgtgat
     2161 ccaccgcacg ctttgtcgcc caccaggcgg agcgaatgac tacccttaaa gaaaagcccg
     2221 ataattagcg acgaatttcg gaggttggat ccttatgctc aatcagaaaa ttcaaaaccc
     2281 taatccagac gaactgatga tcgaagtcga tctctgctat gagctggacc cgtatgaatt
     2341 aaaactggat gagatgatcg aggcagaacc ggaacccgag atgattgaag ggctgcccgc
     2401 ctctgatgcg ctgacgcctg ccgatcgcta tctcgaactg ttcgagcatg ttcagtcggc
     2461 gaaaattttc ccggacagta aaacctttcc cgactgcgca cctaaaatgg acccgctgga
     2521 tattttaatc cgctaccgta aagtgcgccg tcatcgtgat tttgacttgc gcaagtttgt
     2581 tgaaaatcac ttctggctgc cggaggtcta ctccagcgag tatgtatcgg acccgcaaaa
     2641 ttccctgaaa gagcatatcg accagctgtg gccggtgcta acccgcgaac cacaggatca
     2701 cattccgtgg tcttctctac tggcgctgcc gcagtcatat attgtcccag gcggccgttt
     2761 tagcgaaacc tactattggg actcctattt caccatgctg gggctggcgg aaagtggtcg
     2821 ggaagatttg ctgaaatgca tggccgataa cttcgcctgg atgatcgaaa actatggtca
     2881 catccccaac ggcaaccgca cctattattt gagccgatcg caaccaccgg tttttgcgct
     2941 gatggtggag ttgtttgaag aagatggtgt acgcggtgcg cgccgctatc tcgaccacct
     3001 taaaatggaa tatgccttct ggatggacgg tgcagaatcg ttgatcccta atcaggccta
     3061 tcgccatgtt gtgcggatgc cggacggatc gctgctcaac cgttactggg acgatcgcga
     3121 cacgccgcgt gacgaatcct ggcttgagga cgttgaaacc gcgaaacatt ctggtcgccc
     3181 gcccaacgag gtgtaccgcg atttacgcgc gggggcggcc tccggttggg attactcttc
     3241 ccgttggctg cgtgatactg gtcgtctggc gagcattcgt accacccagt tcatccccat
     3301 cgatctgaat gccttcctgt ttaaactgga gagcgccatc gccaacatct cggcgctgaa
     3361 aggcgagaaa gagacagaag cgctgttccg ccagaaggcc agtgcccgtc gcgatgcggt
     3421 aaaccgttac ctctgggatg atgaaaacgg catctaccgc gattacgact ggcgacgcga
     3481 acaactggcg ctgttttccg ctgccgccat tgtgccgctc tatgtcggca tggcgaacca
     3541 tgaacaggcc gatcgtctgg caaacgccgt acgcagccgg ttactgacac ctggcgggat
     3601 tctggcaagc gagtacgaaa ccggtgaaca gtgggataaa cccaacggct gggcaccgtt
     3661 acaatggatg gcaattcagg gatttaaaat gtatggcgat gaccttctgg gtgatgaaat
     3721 cgcgcgcagc tggctgaaaa cggtgaatca gttctatctg gaacagcaca aaatgatcga
     3781 gaaataccat attgccgatg gtgttccccg cgaaggcggc ggtggcgagt atccgttgca
     3841 ggatgggttt ggctggacta acggtgtggt acgccgttta attggtttgt acggcgaacc
     3901 ataatatttt tacagccagc cgctaacttc ctgctggctg taaaattatc ctcttcagga
     3961 g
//
LOCUS       DAJTZL010000263         3949 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.266, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000263 DAJTZL010000000
VERSION     DAJTZL010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3949
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.266"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..952)
                     /gene="ydcO"
                     /locus_tag="OO544_003985"
     CDS             complement(<1..952)
                     /gene="ydcO"
                     /locus_tag="OO544_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HCR6769454.1"
                     /translation="MLRCLYAITHEVTMRLFSIPPPTLLAGFLAVLIGYASSAAIIWQ
                     AAIVAGATTAQISGWMTALGLAMGVSTLTLTLWYRVPVLTAWSTPGAALLVTGLQGLT
                     LNEAIGVFIVTNALIVLCGITGLFARLMRIIPHSLAAAMLAGILLRFGLQAFASLDGQ
                     FTLCGSMLLVWLATKAVAPRYAVIAAMIIGIVIVIAQGDVVTTDVVFKPVLPTYITPD
                     FSFAHSLSVALPLFLVTMASQNAPGIAAMKAAGYSAPVSPLIVFTGLLALVFSPFGVY
                     SVGIAAITAAICQSPEAHPDKDQRWLAAAVAGIFYLLAGLF"
     gene            1005..1541
                     /gene="sutR"
                     /locus_tag="OO544_003986"
     CDS             1005..1541
                     /gene="sutR"
                     /locus_tag="OO544_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HCR6769455.1"
                     /translation="MENLARFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSAMPSVYDPQQQAMVITSLFPYDPQLCFEHF
                     SIQMASGAISESTPHEKGVIEHVVVIDGQLDLCVDGEWQTLNCGEGVRFAADVTHIYR
                     NGGEQTVHFHSLIHYPRS"
     gene            1614..3575
                     /gene="rlhA"
                     /locus_tag="OO544_003987"
     CDS             1614..3575
                     /gene="rlhA"
                     /locus_tag="OO544_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HCR6769456.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLDQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERGDLTRAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVLKVAKDHLNV
                     AVTEPLANGDGLNVMIKREVIGFRANTVEKTGENQYRVWPNEMPADLHKIRPHHPLNR
                     NLDHNWQQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSITHTLDGQFDEANNA
                     EKAMNNLKDGLAKLGQTLYYARDVQINLPGALFVPNSLLNQFRREAADMLDAARLASY
                     QRGSRKPVADPAPVFPQTHLSFLANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDELLKTLPKRKG"
     gene            complement(3667..3897)
                     /locus_tag="OO544_003988"
     CDS             complement(3667..3897)
                     /locus_tag="OO544_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncJ family protein"
                     /protein_id="HCR6769457.1"
                     /translation="MFTKALSVVLLTCALFSGQLMAGHKGHEFVWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGQR"
BASE COUNT         1023 a         1007 c         1001 g          918 t
ORIGIN      
        1 caaacagacc tgcgagcaaa tagaaaatgc ctgcaacggc agctgccagc caacgttgat
       61 ctttatccgg atgcgcttcc gggctttggc aaatagccgc ggtgattgcc gcaataccga
      121 cggaataaac gccgaaaggg gaaaaaacca gtgccagcaa tccagtaaat acaattaatg
      181 gcgaaacagg agccgaatat ccagctgctt tcattgctgc gatacccggt gcgttttgcg
      241 atgccatcgt caccagaaaa agggggagtg caacgctcag gctgtgagca aacgaaaaat
      301 caggggtaat ataagtgggg agaacgggtt taaagacaac atcagttgtg acaacgtcac
      361 cttgcgcgat aacgatcacg atcccaataa tcatcgcggc aattaccgca tagcgcggcg
      421 caacggcctt ggttgccagc cataccagca acatacttcc acacaacgta aattgaccgt
      481 ccagactggc aaacgcctgt aaaccaaagc gtaataaaat cccggcaagc attgccgccg
      541 caagcgagtg cggaataatg cgcatcagac gagcaaagag tcccgttatg ccgcagagga
      601 ctattaacgc gttggtgaca ataaaaacgc cgatggcttc gttaagtgtt agtccctgca
      661 atccggtgac caacaaagcc gcgccaggcg ttgaccatgc ggtgagaaca ggtacgcgat
      721 accataatgt cagagtcagc gtactgacgc ccattgccag ccccagcgcc gtcatccagc
      781 cagagatttg tgcagtggtg gctccggcga caatcgctgc ttgccagatt attgccgctg
      841 aactggcgta gccaattaat accgccagaa accccgccag tagcgtgggt ggaggaatag
      901 aaaacagacg catagtcacc tcgtgcgtta tagcgtacag gcaccgtagc atttgtccgt
      961 tataacgcac aagtgataaa cttccgtttt gccggaggag tcgcatggaa aatctcgctc
     1021 gctttttatc cactacactt aaacaactac gccagcagcg cggctggagt ctttcgcgac
     1081 tggcagaagc gacgggcgtt tctaaagcaa tgttagggca gattgagcgt aatgagtcca
     1141 gtccgacggt cgcgacatta tggaaaattg ccaccgggtt gaacgtacca ttttcaacat
     1201 ttatttctcc gccgcagtcc gctatgcctt cagtttatga tccacaacag caggcgatgg
     1261 tgatcacttc gctgtttcct tatgatccac aactctgctt tgaacacttc tcgattcaga
     1321 tggcatctgg tgcaataagc gaatcgacgc cccatgagaa gggggtaatc gaacatgtcg
     1381 tcgtcattga tggacaactt gatctgtgcg ttgatgggga atggcagacc cttaattgtg
     1441 gggaaggcgt tcgatttgct gcagacgtca cgcatatcta tcgtaatggt ggggagcaaa
     1501 ccgtacattt tcattccctc atccattacc cgcgcagtta agcgggaaaa ctatttcgca
     1561 acgtcgcgct tgtggctaaa atagccgcca tttttcagct actggataag aatgtgaccg
     1621 tatcttctca tcgacttgaa ctgttaagcc cggcacgcga tgccgccatt gcccgcgaag
     1681 ctattttgca cggtgccgat gctgtttata tcggcggccc tggttttggt gcccgtcata
     1741 atgccagtaa tagcctgaaa gatattgccg agctggtgcc gtttgcccat cgttatggtg
     1801 caaaaatttt cgtcacgctt aacaccattt tgcatgatga tgagctggaa cccgcgcaac
     1861 ggctgattac tgacctctac cagaccggtg tcgatgcgct gattgttcag gatatgggga
     1921 ttctggaact tgatattccg ccgattgaac tgcacgccag tacgcagtgc gacattcgta
     1981 cagttgaaaa agcgaagttc ctctctgatg ttggcttcac gcagattgtg ctggcgcgag
     2041 agctgaatct tgatcagatc cgcgcgattc accaggctac ggacgcgacc attgaattct
     2101 ttattcatgg ggcactgtgc gtggcctatt cgggtcagtg ctacatttct catgcgcaaa
     2161 cagggcgtag cgccaaccgt ggcgattgct cgcaggcgtg ccgtttgcca tacacattga
     2221 aagacgatca ggggcgggtg gtttcctatg aaaaacatct gctgtcgatg aaagataacg
     2281 atcagactgc caacctcggc gcgctgattg atgctggcgt acgctccttc aagattgaag
     2341 ggcgttacaa agatatgagc tacgtgaaga atatcaccgc ccattatcgc cagatgctgg
     2401 atgccattat tgaagaacgt ggcgatctga cgcgtgcttc atcaggtcgt actgaacatt
     2461 tctttgttcc atcgacggaa aagactttcc accgtggtag cacagattat tttgtgaatg
     2521 cccgtaaagg cgatattggc gcgttcgatt cgccgaaatt tatcggcctg ccggtgggtg
     2581 aagtattgaa agtggcgaaa gatcatctca atgttgccgt caccgagcca ctggcaaatg
     2641 gtgatggcct gaacgtgatg attaaacgtg aagtcatcgg ttttcgtgcc aatacggtcg
     2701 agaaaaccgg agaaaatcag taccgcgtct ggcccaatga aatgccagca gatttgcaca
     2761 aaattcgtcc acatcaccca ctaaaccgta atcttgatca taactggcag caggcactga
     2821 caaaaacctc cagcgaacgt cgggtggcgg tagacattga actgggcggc tggcaggaac
     2881 aactgattct gaccctcacc agtgaagagg gtgtcagcat cacgcatacg ctggacgggc
     2941 agttcgacga agccaataac gccgaaaaag caatgaacaa tctgaaggat ggtctggcaa
     3001 aactggggca aaccctctat tacgcccgcg atgtgcaaat taatttgccg ggggcgctgt
     3061 ttgtaccaaa cagtctgtta aaccagttcc gccgtgaagc tgctgacatg ctggatgctg
     3121 cgcgtcttgc cagttaccag cgcggcagcc gtaaaccggt tgctgatcct gcgccggttt
     3181 ttccgcaaac gcatctgagt ttcctcgcga acgtatacaa ccagaaagcg cgtgaatttt
     3241 atcatcgcta tggtgtgcag ctgattgacg cggcgtatga ggcacatgaa gagaagggcg
     3301 aagtcccggt gatgatcacc aagcattgtc tgcgctttgc ctttaatctg tgcccgaaac
     3361 aggcgaaagg caatatcaaa agctggaagg cgacgccaat gcaactggtt aacggcgatg
     3421 aagtattaac gctaaagttt gattgccgcc catgcgagat gcacgtcatt ggcaaaatca
     3481 aaaatcacat actgaaaatg ccgttaccgg gaagcgtagt ggcatccgta agtccggatg
     3541 agctgctgaa aacattgcct aagcgaaaag ggtaaaacgc cagttttctg gttactcaca
     3601 actcattgaa tctgcatgat attgcctgcc gggtaaggcg tcacgccgca tccggcatca
     3661 aacgactcaa cgttgtcccg cttctggttt gcgcgatttt tgccagtaaa aatgctcgcg
     3721 caaaccttcc gccgactctt ccgccacagc acgcaattca tcgctgtccg cttcatgacg
     3781 cagctgatga tccacattct ttacccacac aaattcatgt cctttgtgcc ctgccatgag
     3841 ttgtcctgag aacagcgcac acgttaataa gacaaccgat aacgccttcg taaacatcct
     3901 gccacctttt tcttaccttt tgccgctatg atgccgatcg tttcttgag
//
LOCUS       DAJTZL010000264         3916 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.267, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000264 DAJTZL010000000
VERSION     DAJTZL010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3916
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.267"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..302
                     /locus_tag="OO544_003989"
     CDS             <1..302
                     /locus_tag="OO544_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6769458.1"
                     /translation="RVELYYRFNHEEEYYPSHRFMLVVDMKTVMVFSQRAESLGLYSN
                     NRLLINIIAEHIHSDIYLTKYQQQYPDANALINTLHESGNDMVQDEKHLSDSKNR"
     gene            391..2259
                     /locus_tag="OO544_003990"
     CDS             391..2259
                     /locus_tag="OO544_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001548650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HCR6769459.1"
                     /translation="MIFFNDIKPTGWLKNKMEWYMNGHIGELDKLVPHLITEHKIYGR
                     DRITPRTVSAEDGVIKKQNNPDDNMMQYYWWDSETQSNWKDGYCRSAYLLDNKEWQEK
                     ASDLCLELIDTQDDDGYIGIYDSSLKFNCKAENGEFWAQATALRFVLGCYESTRNESL
                     LTSLTQAAQCIMAGYPKETSHPFVVEQGFAGHSHGLTITDVFYRLYEITNNIEYLSYC
                     LWLYEDYSAGCVSEQDLQYKNVIDPGYLLKGHGVHTYEHIRALAIAASQRPQYQTALD
                     IFLSQLKYYLTPSGAPIGDEWITGRTANATYTGYEYCSVHELFSTYVMLIKLTTNIGL
                     ADDAEWLFYNASFGMQHPILHAIMYCKTDNCYEANERKHPDSNQFNTRYKYSPTHEEA
                     AVCCVPNTARTIPAFVENMFQENNNGFTALFYGPCEFYGTFNNVAIKITQLTEYPHDL
                     SVKCLIEPESSVRFALSFRYPGWAKMMIINGVTFTKNDTQNSLIILDRTWQHHDVIDI
                     KFIADIQFNTDLCGDTYISRGPVLYAIELESDILIKKNLLNGKYFDSGYIPCSRADES
                     IQFSYADMESFSYSTSPEGKQYIEGCFYLNKTKIVRKLIPFGQTILRKVTFSNIES"
     gene            2296..>3916
                     /locus_tag="OO544_003991"
     CDS             2296..>3916
                     /locus_tag="OO544_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HCR6769460.1"
                     /translation="MYLTDNIIKLSNKIGQRVYTVVSEMSIEAWITKEPQPFVYRRSG
                     AYQKLTIGSDWGQLFDCAWMRVYGKRPADKFRHKLVALVDIGGEGLIVDKNGSPICGI
                     TNKASSYGVPPDKPGKWVVDLSLVSENDEVEFWIDAACNDLFGYVTNGGIITDVHIAT
                     RNQLLKSLYYDIEVLFDWINDGQKFESIHPKGITPEQFIAKRSERTDEIISTLEYIDN
                     TLITFSNEEIIKCQIAIQSIINKNNNPSEFRIMATGHAHLDIAWMWPLREGRRKAIRT
                     FATALANIEKYPDYIFGASQYQLFHWIKKDYPYFFEKLKKQIAAGRFELQGCFWVECD
                     LNLVSGESLIRQIMHGTRFTLQNFSKKINYVWQPDVFGFPATLPQILKKSGINYIASQ
                     KLSQNKINKFNNYLFRWQGLDGSEILMHNFPEDTYDSRARARSLEYIEQNYNEKEICP
                     YALMVYGVGDGGAGPGEEHIERLTRIRNIDGLPHVDFSRVDKFFTYADAFRESLPIIS
                     GELYFEAHQGCFTSESATKAHNRNMENKLHDA"
BASE COUNT         1230 a          722 c          766 g         1198 t
ORIGIN      
        1 cacgtgtaga actgtattac cgctttaacc atgaagaaga atattaccct tctcaccgtt
       61 ttatgcttgt tgttgatatg aagacagtaa tggttttttc acaacgagct gaatcactgg
      121 ggttatactc gaataatcgc ttgcttatca atataattgc cgaacatatc catagtgata
      181 tatatctaac caagtatcag caacaatatc cagatgccaa tgcattaata aatactttgc
      241 atgaatctgg caatgatatg gtgcaggatg aaaaacattt gagtgatagc aaaaaccgtt
      301 aatattttcc tttcattgtg atcgtctgta ggcgatcaca atctatttgt cttttataac
      361 gtctaaactt ttttatgcaa aggtgtattt atgatttttt ttaacgatat aaagcccact
      421 ggttggttaa agaataagat ggaatggtat atgaacggtc atataggaga actggataaa
      481 ttagttcccc atcttattac ggagcataaa atatatggaa gagatcgtat aactccacgc
      541 actgtatctg ctgaagatgg cgttattaaa aaacagaata atcccgatga taacatgatg
      601 caatattatt ggtgggatag tgaaactcaa tcgaattgga aggatggata ttgccgcagt
      661 gcctacttgt tagataataa agaatggcag gaaaaagcga gcgatttatg tttggaactt
      721 atcgatacgc aagatgatga tggctacatt ggaatttatg attcatcatt aaaatttaac
      781 tgtaaagcag aaaacggaga gttctgggca caagctactg ccttgcgttt tgtgcttgga
      841 tgttatgagt ccacccgtaa tgagagttta ttaacttctc ttacccaagc cgcacaatgc
      901 attatggctg gctaccctaa agaaacgtca catcctttcg ttgtggaaca gggttttgct
      961 ggacatagcc acggattaac aataacagat gttttctacc gcctttatga aataaccaat
     1021 aacattgaat atctctcata ttgcttgtgg ctgtatgaag attattccgc aggatgcgtg
     1081 tcggagcaag atctgcaata taaaaatgtc atagaccctg gttaccttct taaaggtcat
     1141 ggcgtacata cgtatgagca tattagggca ttagccatcg cagcgagcca acggccgcag
     1201 tatcaaactg cattagatat ttttttatcc cagttgaaat attatcttac accatctggc
     1261 gctcccattg gtgatgagtg gattacagga cgcacagcta atgccactta tacaggctat
     1321 gaatattgtt ctgttcatga actatttagc acttatgtta tgttaataaa actgaccact
     1381 aatattggtc tggccgatga tgctgagtgg ctattctata atgctagttt tggcatgcag
     1441 catcccatac ttcacgcgat aatgtactgt aagactgata attgttatga agcaaatgaa
     1501 aggaaacatc ctgacagtaa tcagtttaat accagatata aatactcccc tactcatgag
     1561 gaggctgctg tctgctgcgt accgaataca gcaagaacca taccggcttt tgtcgaaaat
     1621 atgtttcaag aaaataacaa tggttttact gcattatttt atggcccgtg tgagttttat
     1681 ggcacattta ataatgttgc aattaaaatt acacaattaa ctgaataccc tcatgattta
     1741 agcgtaaaat gtctcattga gcctgagtca tctgttcgtt ttgctctcag tttccgttac
     1801 cccggctggg caaaaatgat gattattaat ggtgttactt ttactaaaaa tgatactcaa
     1861 aactcactta ttattttaga tagaacatgg caacatcatg atgtgattga cattaaattt
     1921 atagctgata tacagtttaa tactgactta tgtggtgaca cttatatttc cagaggtccc
     1981 gttttatatg caattgagct tgagagcgat attttaatca aaaaaaacct gctgaacggg
     2041 aaatattttg actcaggata cataccctgt agtagagctg atgaatctat ccagttctct
     2101 tatgctgata tggaatcttt ttcatattca acctcaccag agggcaaaca atacattgaa
     2161 ggctgttttt atttaaacaa gactaaaata gtcagaaaac ttataccatt tggtcaaacc
     2221 attcttcgca aagtaacttt ctccaatata gagagttaat attttcgagt gttataatta
     2281 attaaaggga tttctatgta ccttactgat aatattatca aactgtcgaa taaaataggt
     2341 cagcgtgttt atactgttgt ctctgaaatg tcgattgaag catggataac aaaagaaccg
     2401 cagccttttg tttatcgccg tagcggcgct taccagaaac tcaccattgg ctcagactgg
     2461 ggtcaactgt ttgactgtgc ctggatgcga gtttatggta aacgtccagc agataaattt
     2521 agacacaaac tcgtcgctct cgttgatatc ggtggcgaag gtcttattgt ggataagaat
     2581 ggttcaccca tctgcggcat cacgaataaa gcatcatctt atggcgtacc gccagataaa
     2641 ccaggtaaat gggtggttga cctttctctc gttagtgaaa atgacgaggt tgagttttgg
     2701 attgatgctg cctgcaacga tttatttggc tatgttacta atggagggat tattactgat
     2761 gttcatatcg cgacacgtaa tcaactactg aaatcccttt attatgatat tgaggtgctt
     2821 ttcgactgga ttaatgatgg tcagaagttt gaatccatac atcctaaagg tatcacccca
     2881 gaacaattta ttgccaaaag aagtgagcgt actgacgaaa tcataagtac tctcgaatat
     2941 attgataata cgttaattac ctttagcaat gaagaaataa ttaaatgtca aattgcaatc
     3001 caatccataa tcaataaaaa caataatcct tcagagttca ggattatggc tactggtcat
     3061 gcccatttag atatagcgtg gatgtggcct ttaagagaag gacgcagaaa agcaataaga
     3121 acatttgcca ccgcccttgc aaacattgaa aaatatcctg attacatttt tggcgctagt
     3181 cagtaccaac tttttcactg gataaaaaaa gattatccct atttcttcga aaagttaaaa
     3241 aaacaaatcg ccgcaggccg ctttgagtta caaggttgct tctgggtcga atgcgattta
     3301 aatctggtaa gtggtgaatc gctgataagg caaatcatgc atgggacacg attcacactg
     3361 cagaattttt caaaaaagat aaattatgtc tggcaaccgg atgtctttgg attccccgca
     3421 acgcttccac aaattctcaa aaaatcaggc attaactata ttgcatcgca aaagctctca
     3481 caaaacaaga ttaataaatt taataattac cttttccgct ggcagggcct ggatggctca
     3541 gaaattctga tgcataactt tcctgaagac acttatgata gccgagcacg tgctcgctct
     3601 ttagaatata ttgaacaaaa ctataatgaa aaagagatct gtccctatgc gcttatggtg
     3661 tatggtgtcg gtgatggcgg cgctggcccc ggtgaggaac atatagaacg attaacacgt
     3721 atacgcaata ttgatggctt acctcatgtt gatttcagcc gtgttgataa gttcttcact
     3781 tacgccgacg cctttcgcga aagcttgccc attatttcag gtgaacttta tttcgaggct
     3841 catcaagggt gctttacatc agagtcagca actaaagctc ataatcgaaa tatggaaaat
     3901 aaattacatg atgctg
//
LOCUS       DAJTZL010000265         3910 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.268, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000265 DAJTZL010000000
VERSION     DAJTZL010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3910
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.268"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            26..592
                     /locus_tag="OO544_003992"
     CDS             26..592
                     /locus_tag="OO544_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HCR6769461.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            596..1171
                     /locus_tag="OO544_003993"
     CDS             596..1171
                     /locus_tag="OO544_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HCR6769462.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(1213..2265)
                     /locus_tag="OO544_003994"
     CDS             complement(1213..2265)
                     /locus_tag="OO544_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HCR6769463.1"
                     /translation="MPVLHNRISNDALKAKMLVESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            2490..3410
                     /gene="lpxL"
                     /locus_tag="OO544_003995"
     CDS             2490..3410
                     /gene="lpxL"
                     /locus_tag="OO544_003995"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HCR6769464.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGIQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            3582..3779
                     /locus_tag="OO544_003996"
     CDS             3582..3779
                     /locus_tag="OO544_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HCR6769465.1"
                     /translation="MSPCGNDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSIIPS"
BASE COUNT          971 a          988 c          951 g         1000 t
ORIGIN      
        1 tcgcaaattc gtattggatg cacttatgtc attcacaaat acccctgaac gctatggtgt
       61 tatatctgcg gccttccatt ggctaagcgc aattattgtc tacggcatgt ttgctttagg
      121 tctgtggatg gtcacgctca gttattacga tggctggtat cacaaagcac ccgaactgca
      181 taaaagcatc ggtattttgt taatgatggg gctggttatt cgcgttctgt ggcgtgtcat
      241 atctccaccg ccgggtccgc tgccgagtta ttcgcccatg actcgtcttg ccgcaaaggc
      301 tggacatctc gcattgtacc tattgctgtt cgccatcggc atcagcggct atctgatctc
      361 aactgccgat ggtaaaccga tcagcgtttt tggctggttt gacgtccccg cgacccttgc
      421 cgacgctggc gcacaggcag actttgccgg tgccctgcat ttttggctcg cgtggagcgt
      481 cgtagtactg tccgttatgc acggatttat ggccctgaag catcatttca tcgataaaga
      541 cgacactctg aagcgcatgc tgggaaagtc gtcatctgac tatggagtat aaaaaatgaa
      601 aaaaagcctg cttggtttaa ccttcgcgtc cctgatgttc tctgccggtt cagcggttgc
      661 cgccgattac aaaattgaca aagaaggtca gcacgccttt gttaatttcc gcatccagca
      721 ccttggctat agctggttat acggcacctt taaagatttc gacggtactt ttacctttga
      781 cgaaaaaaat ccggctgccg ataaagtgaa tgtgacaatt aacaccacca gcgtcgatac
      841 taatcacgcc gaacgcgata aacatcttcg cagtgcagat ttcctcaata ccgcaaaata
      901 tccacaggca acattcacct ccaccagcgt gaagaaagac ggtgacgaac tggatattac
      961 cggcgatctg acgctgaatg gcgtaaccaa acccgtcacg ctggaagcga aattaattgg
     1021 tcagggcgac gacccatggg gtggtaaacg tgcaggcttc gaggccgaag gcaaaattaa
     1081 gctcaaggac ttcaatatca agacagattt aggtccagct tctcaggaag tggatctgat
     1141 tatttcagtg gaaggcgtac agcagaagta agcgtgaaat gttgccggat ggcacgcatc
     1201 cggcaatgat atttattctg ttggatcagg aatgcccagt gttgtattca gacgtccacg
     1261 agacttatta aagatcttat tgccattttc acgtcctgcc cgacggcgtc gctgttcttc
     1321 tggcggtaac gcgctttctt cgcagcaaat ctcactacaa caacctttgt atttttccgc
     1381 gcatactgga cactgaataa acagcaggtg gcagccatca tttttacagt tggtatggct
     1441 gtcgcacggc gcgccgcact ggtggcaatg cgcgataatc tcatccgata tacgttcgcc
     1501 catccgctcg tcaaaaacaa aatttttgcc aataaaacgc accggcaagc cctgctcgcg
     1561 cgccttacgg gcgtattcaa taattccgcc ctcgatatgc cagactttgt tgaatccgtt
     1621 atgtttcatc caggcactgg ccttttcaca acgaatgccg ccggtgcagt acatgacgat
     1681 ttttttatct ttatgtgcct gcatcatctc gactgctttt ggcagctgct cacggaaggt
     1741 atctgccgga atttcgagcg cgttttcaaa gtgccccact tcatactcat agtggttacg
     1801 catgtcgata aatagtgcat cgggatcgtc aagcatggcg ttcacttccg ccgcttgcag
     1861 atactcacca acattgctgg catcaaagtg aggatcgtca ataccgtcgg caacaatgcg
     1921 atcgcgtacc ttcatgcgca gtacccagaa ggatttcccg tcatcatcca acgcgatatt
     1981 caggcgtaaa ccctctaaag ccgggtcgaa ggcatagagc tgcgcgcgaa atgtttcaac
     2041 attgctcgca ggtacgctga tttgcgcgtt aatgccctca tgcgccagat acactcgccc
     2101 aaaaacattc agcgcggtaa acagctgata taaagcgtca cgggtcgcct taggatcggc
     2161 gatgtggaaa tacttgtaaa acgaaatggt ggttcgcggt tcgctctcaa ccaacatttt
     2221 ggcttttaac gcgtcgttgg aaatgcggtt gtgtaacact ggcatggtgt acggttcctg
     2281 cgagatggga aagtaaaaat ccgcggcatg atatagcaat tatcgataat taacatccac
     2341 acattttacg ctacatttgc gcattaaaaa ttatttgtta tttacaagcg cggcaatttc
     2401 gcccagtctt cagccacaaa ttttggttgc gggcgaaaaa atgcgacaat acatacaatt
     2461 gcccgaatag gttgaaaaac aggattgata tgacgaatct acccaagttc tccaccgcac
     2521 tgcttcatcc gcgttattgg ttaacctggt tgggtattgg cgtactttgg ttagtcgtgc
     2581 aattgcccta cccggttatc taccgcctcg gttgtggatt aggaaaactg gcgttacgtt
     2641 ttatgaaacg acgcgcaaaa attgtgcatc gcaacctgga actgtgcttc ccggaaatga
     2701 gcgaacaaga acgccgtaaa atggtggtga agaatttcga atccgttggc atgggactga
     2761 tggaaaccgg tatggcgtgg ttctggccgg accgccgaat cgcccgctgg acggaagtga
     2821 tcggcatgga acacattcgt gacgtgcagg cgcaaaaacg cggcatcctg ttagttggca
     2881 tccattttct gacactggaa ctgggcgcgc ggcagtttgg tattcaggaa ccggggattg
     2941 gcgtttaccg cccgaacgat aatccactga ttgactggct acaaacctgg ggccgtttgc
     3001 gctcaaataa atcgatgctc gaccgcaaag atttaaaagg catgattaaa gccctgaaaa
     3061 aaggcgaagt ggtctggtac gcaccggatc atgattacgg cccgcgctca agcgttttcg
     3121 tcccgttatt tgccgttgag caggctgcga ccacgaccgg aacctggatg ctggcacgga
     3181 tgtccggcgc atgtctggtg cccttcgttc cacgccgtaa gccagatggc aaagggtatc
     3241 aattgattat gctgccgcca gagtgttctc cgccactgga tgatgccgaa actaccgccg
     3301 cgtggatgaa caaagtggtc gaaaaatgca tcatgatggc accagagcag tatatgtggt
     3361 tacaccgtcg ctttaaaaca cgcccggaag gcgttccttc acgctattaa atctcccctg
     3421 ccggatgctt cagaatggca tccggcatta ccacagcaaa tccccctgat ttagcgataa
     3481 aagctctctg gattgcgccc cctggaagtc gggcgcataa ttagtgtgct tatcttttct
     3541 tcttatgttc accgcgcctg gcgcaccaac agcggattgc tatgtcaccc tgtggaaatg
     3601 acacccctat aaactggaaa cgaaacctga tcgtcgcctg gctaggctgt tttcttaccg
     3661 gtgccgcctt cagtctggta atgcccttct tacccctcta cgttgagcag cttggcgtta
     3721 ccggtcactc cgccctgaat atgtggtccg gtattgtctt cagtataata ccttcctaaa
     3781 tacactacct gagagaagag atgaaaagag ctatcagcta catgaggttt tcaactgaga
     3841 agcagataaa aggggactct atacgaagac agtctaagct ggtcactgac tggttatcaa
     3901 agaaccctga
//
LOCUS       DAJTZL010000266         3866 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.269, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000266 DAJTZL010000000
VERSION     DAJTZL010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3866
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.269"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            752..1468
                     /gene="nanC"
                     /locus_tag="OO544_003997"
     CDS             752..1468
                     /gene="nanC"
                     /locus_tag="OO544_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HCR6769466.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            1488..2594
                     /gene="nanM"
                     /locus_tag="OO544_003998"
     CDS             1488..2594
                     /gene="nanM"
                     /locus_tag="OO544_003998"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HCR6769467.1"
                     /translation="MNKTIMALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPRDQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSLAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLDPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            2659..3639
                     /locus_tag="OO544_003999"
     CDS             2659..3639
                     /locus_tag="OO544_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709926.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HCR6769468.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHSRIKQL
                     ARFAHTHPGGPPCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPNNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHSYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGIISDRFVEAILQFWRER"
BASE COUNT         1163 a          801 c          877 g         1025 t
ORIGIN      
        1 gacttcagaa gcgaccagtt acaagccaca aatcaggaaa catatttcat ggtgagaatt
       61 atgatcagcc tgatggcggg taaacacttg ttttcaccac gctcactgag ttagcttgca
      121 aagcttccct tgtttagacc ctaaataaat tttttataaa acaaacagtt aaaagaaatc
      181 ctttagtttt tgaagaccac ctcaagtgtt cgtctggctt cacatggcat cttcctctta
      241 gaaaaagatc gacatatttt gtgacacgaa ttgcaaatct cgttttgttg tatggcttgc
      301 gtgatttttg atctggtata acaggtataa aggtgcaccg cgatagtcaa tgagacaggg
      361 catctcgcaa tctatggcaa acatcacttc agttctttct catcgggtga tgaaaacgca
      421 cttcagtctg aaaggaatat gaaaatgaga tcaacagaca ctctatttta tgactctggg
      481 taaaatggat tgagtaagtg atatagctta cgaacattca aatcaattaa acatcagaag
      541 agattttata ctcaggtatt taatctgatt ctctgtttat ttaaataatg tggaaagaga
      601 attttcacag gagaccttat acaaaaatat aaaataagct acccgttgcc aaagacacta
      661 taagcctggc aaaaaatatt acacaacata aatgctaatt gtttatgcgg gctttgtatt
      721 gctttctgta tcctacaaat gagtgaaatt tatgaaaaag gctaaaatac tttctggcgt
      781 attattactg tgcttttcgt caccattaat ttctcaggct gcgacactgg acgtacgtgg
      841 tggatatcgt agcggaagcc atgcctatga gactcgactc aaagtcagtg agggatggca
      901 aaatggatgg tgggcaagca tggaaagtaa tacctggaat accattcatg ataataaaaa
      961 ggaaaatgcc gcactcaatg atgttcaggt tgaagttaat tacgcgatta aacttgatga
     1021 tcaatggacg gtgcgcccgg gaatgttaac gcattttagc agcaacggca cacgctacgg
     1081 accctacgta aaactgtcct gggacgcgac aaaagatctt aattttggca ttcgctatcg
     1141 ttacgactgg aaagcttacc gacaacaaga cttatccggt gatatgtctc gtgataacgt
     1201 tcatcgttgg gatggatatg tcacttacca tattaatagt gatttcacct tcgcatggca
     1261 aacgacgcta tacagcaaac agaatgatta tcgctatgca aaccacaaga aatgggcgac
     1321 ggaaaatgcc tttgttctac aataccatat gacgcccgat attacgccat acatagaata
     1381 tgactacctt gaccgccagg gtgtttacaa cggcagagat aatttatcgg aaaacagtta
     1441 tcgcattggt gtgtcattta aactgtagta gacaggagac agtcacaatg aataaaacta
     1501 taatggcgct tgctatcatg atggcttcat ttgccgcaaa cgcgtctgta ttaccggaaa
     1561 ctcctgtgcc atttaaaagt ggtaccggag caattgataa cgacactgtc tacattggtt
     1621 taggtagcgc aggtacagca tggtacaagc tagatacaca ggccaaagat aaaaaatgga
     1681 ctgcgttagc tgcattccct ggtggaccga gagatcaagc aacctctgca tttattgatg
     1741 gcaatctata tgtgtttggc ggcattggca aaaacagcga gggattgact caggtattta
     1801 atgacgtaca caaatacaac cccaaaacca atagctgggt taaattgatg tcgctcgcgc
     1861 cgatgggcat ggcgggtcat gtgacttttg tacacaacgg caaggcttat gttactggcg
     1921 gtgttaacca gaatatcttc aatggctatt ttgaagatct caacgaggct ggaaaagatt
     1981 caaccgctat agataaaatc aacgcccact attttgacaa aaaagcagaa gattatttct
     2041 tcaataagtt tctgttgtct tttgatccct caacacagca atggagttac gctggcgaat
     2101 cgccctggta cggaacggct ggtgcggcgg ttgtgaataa aggtgataaa acctggctta
     2161 ttaatggcga agccaaacca ggattgcgaa cggatgccgt atttgaactt gatttcaccg
     2221 gtaataattt aaaatggaat aagcttgatc ccgtctcatc accagatggc gtcgctggcg
     2281 gttttgcggg gataagcaat gattctctta tatttgccgg aggggccgga ttcaaaggtt
     2341 cacgagaaaa ttaccagaac ggtaagaact atgcgcatga aggcctgaaa aaatcatata
     2401 gcactgatat tcatctttgg cataacggga aatgggataa atcgggtgaa ttatcgcaag
     2461 gtcgggccta cggagtatca ttgccctgga ataatagttt attaattatt ggcggtgaaa
     2521 ctgcaggcgg caaagcggtg acggattcag ttttaatttc tgtgaaggat aataaagtca
     2581 cagtacaaaa ctaacgcttc agggccccgg taaaggggcc ctgctatcaa tttgtcattt
     2641 gtattacgcg aaaattatat gaacgcaata atatcgcccg attattacta tgttcttacc
     2701 gttgctggtc agtctaatgc catggcgtat ggcgaaggac tgccattacc ggacagggaa
     2761 gatgcgcctc attccagaat taaacaatta gcgagatttg cgcatacgca tcccggaggc
     2821 ccgccatgtc actttaacga cattattcca ctgactcact gcccacacga tgttcaggat
     2881 atgcagggtt atcaccatcc tctggcaacg aatcatcaaa cacagtacgg caccgttggc
     2941 caggcactgc atattgcacg gaaattactg cccttcattc ctaataatgc agggattctt
     3001 atcgttccgt gttgccgtgg cggatcggct tttaccgcgg gcagcgaagg gacatattca
     3061 gaacggcacg gagccagcca tgatgcttgt cgttggggaa cggatactcc gctgtaccag
     3121 gatttagtca gcagaacgcg agccgcactg gcaaaaaatc cgcagaacaa attcctcggc
     3181 gtatgctgga tgcaaggcga atttgactta atgaccagtg actacgcgtc acaccctcaa
     3241 cactttaatc atatggttga agcctttcgt agggatctaa aacaatacca ttctcagctt
     3301 aataatatta ctgacgcgcc atggttttgc ggcgatacca cctggtactg gaaagaaaat
     3361 ttcccgcatt cgtatgaagc tatttatggc aattatcaaa ataatgtttt agccaatatt
     3421 attttcgtcg acttccagca acaaggtgaa agaggactga cgaacgcgcc tgatgaagac
     3481 ccggacgatt taagcacggg atattacggt tcagcgtacc ggtcaccgga gaactggacg
     3541 acggcactgc gaagcagtca tttcagcacg gcagcccgtc gggggattat ttctgacaga
     3601 tttgtagaag caattttgca gttttggcgc gaaaggtgag cggggagttt attcatgggg
     3661 gatgtccttg ttcgttctca gagtagacaa caaaaaacaa ataagatcaa ggtaatatct
     3721 cttcacttgc gggctcgaac agcaggcaat tgccaatata ataatctggc acaagggtga
     3781 aaaacgcgca ccgcataaac cgttgttatt gcttacagat tagccggatg ccttaataga
     3841 catccgcgcc ttttcgatta tggctc
//
LOCUS       DAJTZL010000267         3864 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.270, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000267 DAJTZL010000000
VERSION     DAJTZL010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3864
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.270"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..1803
                     /gene="fimD"
                     /locus_tag="OO544_004000"
                     /pseudo
     CDS             1..1803
                     /gene="fimD"
                     /locus_tag="OO544_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308621.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane usher protein FimD"
     gene            1816..2823
                     /gene="fimH"
                     /locus_tag="OO544_004001"
     CDS             1816..2823
                     /gene="fimH"
                     /locus_tag="OO544_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrin D-mannose specific adhesin FimH"
                     /protein_id="HCR6769469.1"
                     /translation="MKIICRLLLAMACLCLANISWATVCANSTGVAEDEHYDLSNVFN
                     STNNQPGQIVVLPEKSGWVGVSAICPLGTLVNYTYRSYVTNFIVQETIDNYKYMQLND
                     YLLGAMSLVDSVMDIQFPPQNYIRMGTDPNVSQNLPFGVMDSRLIFRLKVIRPFINMV
                     EIPRQVMFTVYVTSTPYDPLVTPVYTISFGGRVEVPQNCELNAGQIVEFDFGDIGASL
                     FSAAGPGNRPAGVMPQTKSIAIKCTNVAAQAYLTMRLEASAVSGQAMVSDNQDLGFIV
                     ADQNDTPITPNDLNSVIPFRLDAAAAANVTLRAWPISITGQKPTEGPFSALGYLRVDY
                     Q"
     gene            2834..3349
                     /gene="sfmF"
                     /locus_tag="OO544_004002"
     CDS             2834..3349
                     /gene="sfmF"
                     /locus_tag="OO544_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria assembly protein"
                     /protein_id="HCR6769470.1"
                     /translation="MRRVLFSCFCGLLWSSSGWAADPLGTININLHGNVVDFSCTVNT
                     ADIDKTVDLGRWPTTQLLNAGDTTALVPFSLRLEGCPPGSVAILFTGTPASDTNLLAL
                     DDPAMAQTVAIELRNSDRSRLALGEASPTEEVDANGNVTLNFFANYRALASGVQPGVE
                     KADAIFMINYN"
     gene            complement(3352..>3864)
                     /gene="fimZ"
                     /locus_tag="OO544_004003"
     CDS             complement(3352..>3864)
                     /gene="fimZ"
                     /locus_tag="OO544_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria biosynthesis transcriptional regulator
                     FimZ"
                     /protein_id="HCR6769471.1"
                     /translation="TIDYLRTRPVDLIIMDIDLPGTDGFTFLKRIKQIQSTVKVLFLS
                     SKSECFYAGRAIQAGANGFVSKCNDQNDIFHPVQMILSGYTFFPSETLNYIKSNKCST
                     NSSTVTVLSNREVTILRYLVSGLSNKEIADKLLLSNKTVSAHKSNIYGKLGLHSIVEL
                     IDYAKLYELI"
BASE COUNT          988 a          846 c          959 g         1071 t
ORIGIN      
        1 tggtgtacgg ctttattctt ccgataatat gtatccagat agccagcaag ggtttgcccc
       61 aacggtacgt gggattgccc gtacggcggc ccagctaacg attcggcaaa atggttttat
      121 tatctatcaa agctatgttt cccccggtgc ttttgaaatt actgatttgc acccgacatc
      181 ttcaaatggc gatctggatg tcaccatcga cgagcgcgat ggcagaatta cacaattccg
      241 tattcaacag tgccgatttt acaacgcgaa gggcgtttca aatttgacct gacggcgggc
      301 gattttcgta gcggtaatag tcagcaatca tcacctttct tttttcaggg cacggcactc
      361 ggtggtttac cacaggaatt tactgcctac ggcgggacgc aattatctgc caattacacc
      421 gcctttttat tagggctggg gcgcaacctc gggaactggg gcgcagtgtc gctggatgtg
      481 acccatgcgc gcagccagtt agccgacgac agtcgtcatg agggggattc cattcgcttc
      541 ctctatgcga aatcgatgta caccttcggc accaattttc agttaatggg ttaccgctat
      601 tcgacacaag gtttttatac ccttgatgat gttgcgtatc gtcgaatgga ggggtacgaa
      661 tatgattacg attacgacgg tgagcatcgc gatgaaccga taatcgtgaa ttaccacaat
      721 ttacgcttta gccgcaaaga ccgtttgcag ttaaatattt cacaatcact taatgacttt
      781 ggctcgcttt atatctctgg tacccatcaa aaatactgga atacttcgga ttcagatacc
      841 tggtatcagg tggggtatac cagcagctgg gttggcatca gttattcact ctcattttcg
      901 tggaatgaat ctgtagggat ccccgataac gaacatattg tcggacttaa tgtttcagtg
      961 cctttcaatg ttttgaccaa acgtcgctac acccgggaaa atgcgctcga ccgcgcttat
     1021 gcctccttga acgctaaccg taacagcaac gggcaaaata gctggctggc aggcgttggt
     1081 gggaccttac tggaaggcca caacctgagt tatcacgtaa gccagggcga tacctcgaat
     1141 aatgggtaca cgggcagcgc cacggcaaac tggcaggcca cttacggtac gctgggggtc
     1201 gggtataact acgaccgcga tcaacatgac gttaactggc agctgtctgg cggtgtggtc
     1261 gggcatgaaa atggcataac actgagccag cctttagggg ataccaatgt tttgattaaa
     1321 gcgcttggcg caggcggtgt acgcattgaa aatcaaactg gcattttaac cgactggcgc
     1381 ggctatgcgg tgatgccgta tgccacggtt tatcggtata accgtatcgc gcttgatacc
     1441 aatacgatgg ggaattccat cgatgttgaa aaaaatatta gcagcgttgt gccgacgcaa
     1501 ggcgcgttgg ttcgtgccaa ttttgatacc cgcataggcg tgcgggcgct cattaccgtt
     1561 acccagggcg gaaaaccggt gccgtttgga tcactggtac gggaaaacag taccggaata
     1621 accagtatgg tgggtgatga cgggcaagtt tatttaagtg gtgcgccatt gtctggtgaa
     1681 ttactggttc agtggggaga cggcgcgaac tcacgctgca ttgcgcacta tgtattgcca
     1741 aagcaaagct tacagcaagc cgtcactgtt atttcggcag tttgcacaca tcctcgctca
     1801 taaaggaaat tatcaatgaa gataatctgc agattattat tggcaatggc atgtttgtgt
     1861 ctggcaaaca tatcctgggc tactgtttgt gcaaatagta ctggcgtagc agaagatgaa
     1921 cactatgatc tctcaaatgt ctttaatagc accaataacc agccagggca gattgttgtt
     1981 ttaccggaaa aatccggctg ggtaggtgtc tcagcaattt gtccactcgg cacgctggtg
     2041 aattatacat accgtagtta tgtcactaac tttattgttc aggaaactat cgataattat
     2101 aaatatatgc aattaaatga ttatctatta ggtgcgatga gtctggttga tagtgtgatg
     2161 gatattcagt tccccccgca aaattatatt cggatgggaa cagatcctaa cgtttcgcaa
     2221 aaccttccgt tcggggtgat ggattctcgt ttgatatttc gtttaaaggt tattcgtccc
     2281 tttattaaca tggtggagat ccccagacag gtgatgttta ccgtgtatgt gacatcaacg
     2341 ccttacgatc cgttggttac acctgtttat accattagtt ttggtggccg ggttgaagtg
     2401 ccgcaaaact gcgaattaaa tgccgggcag attgttgaat ttgattttgg tgatatcggc
     2461 gcatcgttat ttagtgcggc agggccgggg aatcgaccag ctggcgtcat gccgcaaacc
     2521 aagagcattg cgatcaaatg tacgaatgtt gctgcgcagg cttatttaac aatgcgtctg
     2581 gaagccagtg ccgtttctgg tcaggcgatg gtgtcggaca atcaggattt aggttttatt
     2641 gtcgccgatc agaacgatac gccgatcacg cctaacgatc tcaatagcgt tattcctttc
     2701 cgtctggatg cagctgcggc agccaatgtc acacttcgcg cctggcctat cagtattacc
     2761 ggtcaaaaac cgaccgaagg gccgtttagc gcgctggggt atttacgcgt cgattatcaa
     2821 tgaggtacgg agaatgagaa gagtactctt tagctgtttc tgcgggttac tgtggagttc
     2881 cagtggatgg gcagctgacc ctttgggaac gattaatatc aatttgcacg gtaacgttgt
     2941 tgatttctcc tgtaccgtaa acactgcgga tattgataag acggtagatt taggcagatg
     3001 gcctacgaca caattactga acgctggcga taccacggca ctcgtccctt ttagcctgcg
     3061 gctggaggga tgtcctccgg gttccgttgc gattttattt acgggaacgc cggcatccga
     3121 taccaacctg ctggctctgg atgatcccgc aatggcacaa accgtcgcca tcgaattacg
     3181 taatagcgat cgctcccggc tcgcactggg ggaggcgagc ccgactgagg aagtagatgc
     3241 aaatggcaat gtcacactaa acttttttgc caattatcga gcgttagcca gcggtgttca
     3301 gccaggtgtg gagaaagcgg atgcgatatt tatgatcaat tataattaat attatattaa
     3361 ttcgtataat ttggcgtagt cgataagctc tacaattgaa tgcaaaccta gtttgccata
     3421 aatattagat ttatgcgcac taactgtttt attgctaagt aataacttat cggcaatttc
     3481 tttattagat aatccgctaa ccagataacg taatatggtc acttcacgat tagatagcac
     3541 agtgaccgtt gaactattcg tactacattt attgcttttt atatagttga gcgtttcgct
     3601 aggaaaaaac gtgtatccgg agaggatcat ctgaacggga tgaaaaatat cattctgatc
     3661 attgcattta ctgacaaaac cgttagcacc agcttgtatc gctctgccag cataaaagca
     3721 ttctgatttc gatgataaaa ataacacttt cactgtgctc tggatttgtt tgatcctttt
     3781 caggaaggta aaaccgtctg ttccgggcaa gtctatatcc ataatgatta aatcaacagg
     3841 acgggttcgg agataatcga tggt
//
LOCUS       DAJTZL010000268         3853 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.271, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000268 DAJTZL010000000
VERSION     DAJTZL010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3853
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.271"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            115..510
                     /gene="caiF"
                     /locus_tag="OO544_004004"
     CDS             115..510
                     /gene="caiF"
                     /locus_tag="OO544_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HCR6769472.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVAEISCAVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSRDKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(719..1309)
                     /gene="caiE"
                     /locus_tag="OO544_004005"
     CDS             complement(719..1309)
                     /gene="caiE"
                     /locus_tag="OO544_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HCR6769473.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFHGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(1315..2100)
                     /gene="caiD"
                     /locus_tag="OO544_004006"
     CDS             complement(1315..2100)
                     /gene="caiD"
                     /locus_tag="OO544_004006"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HCR6769474.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFDGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIFRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            complement(2209..3762)
                     /gene="caiC"
                     /locus_tag="OO544_004007"
     CDS             complement(2209..3762)
                     /gene="caiC"
                     /locus_tag="OO544_004007"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HCR6769475.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTTNLFYTLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESA
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYL
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQVQKYRATVTECIPMMIRTL
                     MAQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPG
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYCDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
BASE COUNT          909 a         1047 c          941 g          956 t
ORIGIN      
        1 ttatcgacgg tgaagttgca tactatcgat atatccacaa ttttaatatg gccttgttta
       61 attgcttcaa aacgagtcat agccagactt ttaatttgtg aaactggagt tcgtatgtgt
      121 gaaggatatg ttgaaaaacc actctacttg ttaatcgccg aatggatgat ggctgaaaat
      181 cggtgggtga tagcaagaga gatctctatt catttcgata ttgaacacag caaggcggtt
      241 aataccctga cttatattct gtcggaagtc gcagaaataa gctgcgcagt taagatgatc
      301 cctaataagc tggaaggacg gggatgccag tgccagcgac tggtaaaagt ggtcgatatc
      361 gatgagcaaa tttacgcgcg cctgcgcaat aacagccggg ataaattagt cggcgtaaga
      421 aagacgccgc gtattcctgc cgttccgctc acggaactta accgcgagca gaagtggcag
      481 atgatgttgt caaagagtat gcgtcgttaa tgtcatttcg ttgataccac gcagcctggt
      541 tgctggatgc aatgctggcg catcttatcc agcctacggt tgctctccag ccgggttgga
      601 taaggcgttt atgccgcatc cgacagctat cgccggatgc gatgctggcg catcttatcc
      661 ggcctacggt tgctctccag cagagttgga taaggcgttt acgccgcatc cggcaagatc
      721 accgtttagg cgtcacatcc gtcgtcccct gcaaacgggg gcgattttcc tccatttgcc
      781 tcagtggctg tgtttcatgt aacgatgcat gacagcgccc gacaagatcc tgatactctt
      841 tggtattcaa ccgtttccag tgtaactcgt cgtcactaac actgcgtaca gcgcgggctg
      901 gcgtacccat caacaactgg cgtttctcgc cgtgaaagcc tgctttgaca aagctcatgg
      961 cggcaacaat gctctcttcg ccaatgaccg cgccatccat aatcacgctg ttcatcccga
     1021 ccaatgcatc gcgaccaatc acacaaccat gcaggatcgc tccgtgcccg atatggccgt
     1081 tttccccaac gatggtgtca gtgtcgcagt agccatgcat aatgcagcca tcctgaatat
     1141 tggctcccgc ttgcacgatc aaccgcccgt agtcaccacg cagtgaggcg agtgggccga
     1201 tgtagacacc ggctcccaca atcacatcgc caatcaagac ggcactggga tggacaaacg
     1261 ccgtcgggtg aaccaccgga attaacccct caaaggcgta atagctcacg gttgttaacg
     1321 tcctttccac accggatcgc gcttctcggc aaacgccagc ggcccttcaa tggcatcctc
     1381 cgaatgcaga accgacggat agtgtttcaa cacgccgctg cgaatatagc gatacgcttc
     1441 ttctaccggc atttcgctgg tggtgcggaa gatctctttc agcgccgcaa tcgccagcgg
     1501 ggcgctgtta accagctgct gcgccagttc gcgggcgtta tccatcagtt ccgcctggct
     1561 aaccacgcgg ttgactatcc cccaacgcag cgcctcttct gcgcccattc gtctgccggt
     1621 catcaccatt tcattgacga tggcaggcgg caggatcttc ggtagacgca gcacgccgcc
     1681 gctgtcagga acgatgccca gcttggcttc cggcagggcg aagctggcgt tatcggcaca
     1741 aacaataaaa tctgccgcca gcgccagttc gaaaccgccg tcaaaggcat agccgttcac
     1801 agctgcgata accggtttgt cgagattgaa aatttcggtt aatcccgcaa atccacccgg
     1861 gccaaagtca gcatccggtg cttcgccttc tgctgccgct tttaaatccc agcccgcgga
     1921 aaagaacttc tctccggcac cggtaataat ggcgacacgt aattgcggat cgtcacggaa
     1981 atttagaaat acttcgccca tttcaaagct ggtttttgca tcaatagcat tcgcttttgg
     2041 acgatcaagg gtaatttcca gaattgatcc attgcgggtc agatgtaatg attcactcat
     2101 tccttttctc catttttgct ttttcaggga cgacaacatc cctgcaaaaa atgcatattg
     2161 ttttatagcg tgattattag ttggcagggt agttccctgc tgtttcattt atttcagatt
     2221 ctttctaatt attttccccg agcaattacg tggcagatct tttctgatct ccagataaga
     2281 gggcacttta aatttcgcca tattttgttc gcagaagcgg aaaaattcct cttcgctcaa
     2341 tgtttcacct tcattcagca ccacaaatgc tttgatggct tcatcgcgaa tcgaatcttt
     2401 aatacccaca accacgatat cctgaatttt tggatgggtg gcgatgatat tttccagctc
     2461 cacgcaggag acattctcgc caccgcgttt gatcatattg cagcggcgat cgacgaaata
     2521 aaaaaagccc tcttcgtcgc agtatccggt atcgccggta tgtagccagc cgtcggcttc
     2581 cagcactttc gcagtggctt tcgggttgag aaaatactct ttgaagatgg ttttccctgg
     2641 tacgccttta atacagattt caccgatctc acctgccggg agcgggcgat tgtgatcgtc
     2701 gcggatctcc gcttcgtagc aaaaccccgc ccgaccaatc gacggccagc gtcgtttatc
     2761 gccagggcga tcgccgataa tgcccacaat ggtttccgtc atcccataag acgtcagcaa
     2821 gcgaacgccg aagcgttcac aaaacgcatc tttttcctgc tccgacaagt tgagataaaa
     2881 catcacttcc cgcaggcggt gttgccgatc gttcgctgaa ggcggctgcg ccatcaaggt
     2941 acggatcatc atcggaatac attcggtaac ggtggcgcgg tacttctgta cctgtcccca
     3001 gaaggcgcgg gcgctgtatt tctcgaccag cacaaaggtg gccccggcag aaaacgccgc
     3061 catcgccgca gtacactggc aatcgatatg aaacgcaggc attaccgtca ggtagacgtc
     3121 atcgtcacgc agtgcacact gccaggcgga gtaatatcca gcgaagcgca ggttgtaatg
     3181 ggtaatcacc acacctttcg gtcgggaggt ggtgccggag gtgaagagaa tttccgccgt
     3241 atcgtcagtc gatagcggcg gtgcatagca caaggtggca ggttgttgat ttttcagttg
     3301 agtaaacgaa ctcacgccat catcagcggg aagtgccaca tctgtcaggc aaatgtgccg
     3361 caattgagtg gcatcttcct gctgaatctg ttgatacata ggatagaatt gcgcactggt
     3421 caccagcagg cacgcctggc tattttgcag gatccacgcg ctttcctcgc gcaacaggcg
     3481 ggcgttaatc ggcaccataa tcgcgccaat ttttgccagc ccgaaccagc aaaagataaa
     3541 ttccgggcag ttgtcgagat gtagagcaac cttgtcgcct ttgcgaatcc ccagcgtata
     3601 aaacaggttt gtcgtgcggt taatctcctg atttaactca agataactat accggttaac
     3661 gactccgccg ctggattcac aaatcagcgc cgttttatga ccgtaaacgt ccgccagatc
     3721 gtcccacatt tgacgtagat gttgtccgcc aatgatatcc attgcacctc tatccatttt
     3781 tgttcgtttg ttattgggcg ggcgctagtc aggcaagccg actgacgcca cgcgtttagt
     3841 cctcaacttt ggc
//
LOCUS       DAJTZL010000269         3833 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.272, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000269 DAJTZL010000000
VERSION     DAJTZL010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3833
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.272"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..267)
                     /locus_tag="OO544_004008"
     CDS             complement(<1..267)
                     /locus_tag="OO544_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HCR6769476.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRELTDAGGFLDISLD"
     gene            354..761
                     /gene="nudG"
                     /locus_tag="OO544_004009"
     CDS             354..761
                     /gene="nudG"
                     /locus_tag="OO544_004009"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HCR6769477.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFADGKVELDES
                     QQQALVRELNEELGIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARAAD"
     gene            complement(727..999)
                     /locus_tag="OO544_004010"
     CDS             complement(727..999)
                     /locus_tag="OO544_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HCR6769478.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLLWRRVKP"
     gene            1235..2578
                     /gene="gdhA"
                     /locus_tag="OO544_004011"
     CDS             1235..2578
                     /gene="gdhA"
                     /locus_tag="OO544_004011"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HCR6769479.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQIQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            complement(2695..3734)
                     /locus_tag="OO544_004012"
                     /pseudo
     CDS             complement(2695..3734)
                     /locus_tag="OO544_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
BASE COUNT          998 a          932 c          917 g          986 t
ORIGIN      
        1 atcaagagaa atatcaagaa agccgcccgc gtcggtaagc tctctgcgcc gcgccagcgc
       61 accgtccaga ccatcaagca gcctgttcaa caaaatgacg actagcgccg ccagatacca
      121 gcccagcgcc agaaacggca gagccagcac gccgatggca aatcccacta atgttaaacc
      181 atctggcgta atgcccggtt tatcaagaac ccgcacgcac tgatgcaaca acggtttaat
      241 ccggggatga agatggcggt ctagcacagg cactccttaa atataaagcc tttctgattg
      301 agcaacagtg cggatattat ggcatttttc gcttatctgc ccgtgtgtaa tttatgaaaa
      361 tgattgaagt tgttgccgcc atcattgaac gtgatggcaa aattttactc gcgcaacgcc
      421 ccgcccagag cgatcaggcg ggattatggg agtttgccga tggtaaagtc gagctggatg
      481 aaagccagca gcaggcactg gtgcgtgagt taaacgaaga attgggcatc gaagcaactg
      541 tgggtgagta tgttgccagc catcagcgag aagtttcggg ccggattatc catcttcatg
      601 cctggcacgt acccgacttc cacgggacgt tacaggcaca tgaacatcag gcgctggtct
      661 ggtgctcacc tgaagaggcg ctgcaatatc cgctggcccc tgctgacatt ccactattag
      721 aggcgtttat ggctttacgc gccgccagag cagcggatta gtgctaaggg ttttgtcatc
      781 ccgctggcat tgcagcagta ttccttcggc tttaattacc gccccttcag aataattttg
      841 atcctgataa acgcagcact gagtacaggg ctgcgctgac tgcccgcctg aactgaacac
      901 ttctggcggt acgtttacct ccacgtccgg acgataatgc gggttagcca gtgcaattaa
      961 tggaaatgct aatactacgg cgaacaatgc tcgactcaca gggaactcct taacgttatt
     1021 gtctctgcaa ctgataacgg tagccgggtg gcaaaacttt agcgtctgag ttatcgcatt
     1081 tggttatgag attactctcg ttattaattt gctttcccgg gtcatttttt tcttgcttac
     1141 cgtcacattc ttgatggtat agtcgaaaac tgcaaaagca catgacataa acaacataag
     1201 cacaatcgta ttaatatata agggttttat atctatggat cagacatatt ctctggagtc
     1261 attcctcaac catgtccaaa agcgcgaccc gaatcaaacc gagttcgcgc aagccgttcg
     1321 tgaagtaatg accacactct ggccttttct tgaacaaaat ccaaaatatc gccagatgtc
     1381 attactggag cgtctggttg aaccggagcg cgtgatccag tttcgcgtgg tatgggttga
     1441 tgatcgcaac cagatacagg tcaaccgtgc atggcgtgtg cagttcagct ctgccatcgg
     1501 cccgtacaaa ggcggtatgc gcttccatcc gtcagttaac ctttccattc tcaaattcct
     1561 cggctttgaa caaaccttca aaaatgccct gactaccctg ccgatgggcg gtggtaaagg
     1621 cggcagcgat ttcgatccga aaggaaaaag cgaaggtgaa gtgatgcgtt tttgccaggc
     1681 gctgatgacc gagctttatc gtcatctggg cgcggatacc gacgttccgg caggtgatat
     1741 cggcgttggt ggtcgtgaag tcggctttat ggcggggatg atgaaaaagc tctccaacaa
     1801 taccgcctgc gtcttcaccg gtaagggcct ttcatttggc ggtagtctta ttcgcccgga
     1861 agctaccggc tacggtctgg tttatttcac agaagcaatg ttaaaacgcc acggtatggg
     1921 ttttgaaggg atgcgcgttt ccgtttctgg ctccggcaac gtcgcccagt acgctatcga
     1981 aaaagcgatg gaatttggtg ctcgtgtgat cactgcgtca gactccagcg gcactgtagt
     2041 tgatgaaagc ggattcacga aagagaaact ggcacgtctt atcgaaatca aagccagccg
     2101 cgatggtcga gtggcagatt acgccaaaga atttggtctg gtctatctcg aaggccaaca
     2161 gccgtggtct gtaccggttg atatcgccct gccttgcgcc acccagaacg aactggatgt
     2221 tgacgccgcg catcagctta tcgccaatgg cgttaaagcc gtcgctgaag gggcaaatat
     2281 gccgaccacc atcgaagcga ctgaactgtt ccagcaggca ggcgtactgt ttgcaccggg
     2341 taaagcggct aatgctggcg gtgtagcaac gtcgggcctg gaaatggcac aaaacgctgc
     2401 gcgcctgggc tggaaagccg agaaagttga cgcacgtttg catcacatca tgctggatat
     2461 ccaccatgcc tgtgttgagc atggtggtga aggtgagcaa accaactacg tgcagggcgc
     2521 gaacattgcc ggttttgtga aggttgccga tgcgatgctg gcgcagggtg tgatttaagt
     2581 tgtaaatgcc tgatggcgct acgcttatca ggcctacaaa tgggcacaat tcattgcagt
     2641 tacgctctaa tgtaggccgg gcaagcgcag cgcccccggc aaaatttcag gcgtttatga
     2701 gtatttaacg gatgatgctc cccagggaac atttcttatg ggccaacggc atttcttact
     2761 gtagtgctcc caaaattgct tgtcgtaacg ataacacgct tcaagttcag catccgttaa
     2821 ctttctgcga tagcagcaga tatgccagta aagaaacccc attagactat ttttttgata
     2881 atcttcttcg ctttcgaaca actcgtgcgc ctttcgagaa gcaagcatta tataatgcca
     2941 ggccagttct tcttcaattg tcccgttttg aaaagctgtg cttgatatcg aggtcatcca
     3001 tgataattcc gccgcccata ttagcttcgc cgaggattta ccggagcgat tattagcgca
     3061 atcagagata tagtctgagg gaaaaacagc aaatttattc aacaaggcga taacctgctc
     3121 tggggcttcc tccatgtttg ctttaaaggt attggctcca tggtcgccag aaagaaaatg
     3181 ctccattagg cacaataact ttcgctatct tcgatacccc attgattctc taaagactcg
     3241 cgtcttttac tgatgatatc gatcgagtca aaaggaagca catgatattg gaaggtatct
     3301 ttaccaggtt caggctttcg cggccagaac tctagcgttt cagaccattg cttatgatag
     3361 aatcgataag gtgcgatcaa ttgtagcgcc tgtaacttct cgatactgag cggctcaata
     3421 cctttagcct gataataatg cagttgttct ttttttgctt taaaaccggc ccgaacaata
     3481 agccccatca taattaatag ataaagaaaa gagcatcccg cggtaatcag gcctctttca
     3541 ttcaaaccgt tggatgttat cgctgcgaac acaaacatta cagcgacaac acatgttaaa
     3601 taaaaccccc acttacaaag cagcatggcc ttattttctt taatcatccg ttcaaaatta
     3661 ctattaaata tttcccagcc attaaaagaa tacttctcgc tcccaggatg gttttgtaat
     3721 aaaacttttt tcatcacatt ccctgttatt acatacttta gaggcgataa ttttatccat
     3781 gcaaaaaaaa tatccaacga attttcttga tccgctggat atttcagaat atg
//
LOCUS       DAJTZL010000270         3802 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.273, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000270 DAJTZL010000000
VERSION     DAJTZL010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3802
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.273"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..577
                     /gene="yeaG"
                     /locus_tag="OO544_004013"
     CDS             <1..577
                     /gene="yeaG"
                     /locus_tag="OO544_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HCR6769480.1"
                     /translation="IEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSE
                     YGQNIFDRYVTYADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNE
                     IVNFVLRARANNSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKH
                     DDFVDRMMEKGYTRKQVRLLCEWYLRVRKSS"
     gene            690..1973
                     /locus_tag="OO544_004014"
     CDS             690..1973
                     /locus_tag="OO544_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HCR6769481.1"
                     /translation="MTGFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKLLPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
     gene            2120..3595
                     /locus_tag="OO544_004015"
     CDS             2120..3595
                     /locus_tag="OO544_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HCR6769482.1"
                     /translation="MIQSTRISMGLFFKYFLSLTKIDPGQNYISLPSIKSSTHIALLF
                     MVSMGTQKLKAQSFFIFSLLLTLILFCITTLYNENTNVKLIPQMNYLMVVVALFFLNA
                     VIFLFMLMKYFTNKQILPTLILSLAFLSGLIYLVETIVIIHKPINGSTLIQTKSNDVS
                     IFYIFRQLSFICLTSLALFCYGKDNILDNNKKKTGILLLALIPFLVFPLLAHNLSSYN
                     ADYSLYVVDYCPDNHTATWGINYTKILVCLWAFLLFFIIMRTRLASELWPLIALLCLA
                     SLCCNLLLLTLDEYNYTIWYISRGIEVSSKLFVVSFLIYNIFQKLQLSSKLAVHDVLT
                     NIYNRRYFFNSVESLLSRPVVKDFCVMLVDINQFKRINAQWGHRVGDKVLVSIVDIIQ
                     QSIRPDDILARLEGEVFGLLFTELNSAQAKIIAERMRKNVELLTGFSNRYDVPEQMTI
                     SIGTVFSTGDTRNISLVMTEADKALREAKSEGGNKVIIHHI"
BASE COUNT         1067 a          856 c          829 g         1050 t
ORIGIN      
        1 gatcgagcgc gagcagttcc cgcaagagca ggcagaacgc tatctggagt tcctgaaagg
       61 ttatctgatc ccgaaatatg ccgagtttat cggcaaagag atccagacgg cctaccttga
      121 atcctattcc gaatatgggc aaaacatttt cgaccgttat gttacctacg cggatttctg
      181 gattcaggat caggagtatc gcgatccgga taccgggcag ctgtttgacc gcgagtctct
      241 taacgccgag ctggagaaaa tcgagaaacc ggcggggatc agtaatccaa aagatttccg
      301 caacgagatt gttaacttcg tactgcgcgc cagagcgaat aacagcggac gcaatccgaa
      361 ctggaccagc tatgaaaaac tgcgcacggt tatcgagaag aaaatgttct ccaataccga
      421 ggagctgttg ccggttatct cgtttaacgc caaaacgtca accgacgagc agaagaaaca
      481 cgacgacttt gtcgaccgta tgatggaaaa aggctacacc cgtaaacagg tgcgtttact
      541 gtgcgaatgg tatttgcgcg tacgtaaatc gtcttaacaa ccctggcccg gtacacatgc
      601 taccgggcct ataacgacag cgaaccgtgg gccagagaaa tggcaacaca ggcgtagcat
      661 acagttggca aatgtagtac ggggggcata tgaccgggtt tattgaccgg cgtctgaacg
      721 gcaaaaacaa aagcatggtg aatcgccagc gttttttacg ccgttataaa gcgcaaatta
      781 aacagtcgat ctccgaggcc attaataagc gttcggtgac tgacgtcgac agcggcgaat
      841 ccgtatccat tcccacggaa gatattagcg aaccgatgtt tcatcagggg cgtggcggcc
      901 tgcgccaccg cgtacatccg ggcaatgacc atttcgtcca gaacgaccga attgaacgtc
      961 cccagggtgg cggcggaggt tccggcagtg gtcagggcca ggccagccag gatggtgaag
     1021 gtcaggatga atttgtcttt cagatttcga aagatgagta tcttgatctg ctctttgaag
     1081 atttggcctt accgaatctg aaacaaaacc aacaacgcca gctgaccgaa tataaaacgc
     1141 atcgggcggg ttataccgct aacggcgttc cagccaatat cagcgttgtg cgttccttgc
     1201 agaactcact ggcgcgacgc acagccatga cggcgggcaa gcgacgggaa cttcatgcgc
     1261 tggaagagaa tttggccatc atcagcaaca gtgaacctgc gcaactgctg gaagaggaac
     1321 gtctgcgcaa agaaattgca gaattacgtg ccaaaattga acgcgtccct tttattgaca
     1381 ccttcgattt acgttacaag aactacgaga agcggcccga tccctccagc caggcagtga
     1441 tgttttgcct gatggacgtt tccggttcaa tggatcaatc cactaaagat atggctaagc
     1501 gtttttatat tctgctgtat ctgttcctca gcagaacgta taagaacgtg gaagtcgtat
     1561 acatccgcca tcatacccag gcgaaagaag tcgatgaaca tgagtttttc tactcgcagg
     1621 aaacaggcgg caccattgtt tccagcgccc tgaaactgat ggatgaggta gtgaaagagc
     1681 gttataaccc ggcacagtgg aatatttacg ctgcacaagc atcggacggc gataactggg
     1741 ccgatgactc tccgctttgc catgaaatcc tggcgaaaaa attattacct gttgttcgtt
     1801 attacagcta tatcgaaatt acccgtcgtg cacatcagac attgtggcga gaatatgagc
     1861 atctgcaatc tactttcgac aactttgcga tgcagcacat ccgcgaccag gatgatattt
     1921 atccggtgtt ccgtgaactg tttcataaac aaaatgcaac agctaaagac taaaactatc
     1981 agccaggtca ttatcgcctg gctgattttt agcttactgt aaattatctc atttattaca
     2041 tacattagct tacaatcgct ttaaatatga cagcataacc tttacataat ttagttccag
     2101 aaaacaatca ttcggaaaaa tgattcagtc aacacgtatt tccatggggt tattctttaa
     2161 atatttttta tcgttaacga aaattgatcc tggtcaaaac tatatatctc tgccatcaat
     2221 aaaatccagc actcacattg ctctcctttt tatggtttct atgggtacac aaaaattaaa
     2281 agctcaaagc ttttttattt tcagtttatt gctgacgtta attttatttt gcattactac
     2341 cttatataac gaaaacacaa atgtaaaact catcccacag atgaattacc tgatggttgt
     2401 tgtggctttg tttttcctta acgccgtcat ttttcttttc atgttaatga aatatttcac
     2461 taacaaacaa attttaccaa cactcatttt aagccttgca tttttaagtg gccttatcta
     2521 tttagttgaa accattgtaa ttatccataa accaattaac ggcagtacac tgatccagac
     2581 aaagtcgaat gatgtttcta ttttctatat tttccgccaa ctcagtttta tttgtttaac
     2641 ctcgctggca ctcttttgtt atggaaaaga caacatcctt gacaacaata agaaaaaaac
     2701 gggaatcctg ttgctggcgc tgattccttt tttagttttt ccccttctgg cacacaatct
     2761 gagcagttat aacgctgact attctttgta tgtcgtcgac tactgtccgg acaaccatac
     2821 tgcgacctgg ggaatcaact atacaaaaat attggtttgt ctgtgggcat ttttactgtt
     2881 ctttattatc atgcgtacac gattagccag cgaactatgg ccgttaatag cattattatg
     2941 tctggcatcg ctatgctgca acttacttct actgactctg gatgagtata attacaccat
     3001 ctggtatatc agtcgcggga ttgaagtttc cagtaaactg tttgttgtgt cttttctgat
     3061 ttataacatt tttcagaaac tgcaactctc cagcaaactg gcagttcatg atgtgctgac
     3121 caatatttat aatcggcgct actttttcaa cagcgtagag tcattattgt cgagacctgt
     3181 tgttaaggac ttctgtgtca tgctggttga tattaatcag ttcaaacgca tcaatgccca
     3241 atggggacat cgtgtgggtg ataaagtgct ggtttcaatt gtcgatatta tccagcaaag
     3301 catccgcccc gatgatattt tagcgcgact ggagggtgag gtgtttggct tgctatttac
     3361 cgaactcaat agtgcccagg caaaaatcat tgcggaacgt atgcgtaaaa atgtcgaact
     3421 cctgaccggc tttagtaaca gatatgatgt tcctgaacaa atgaccatca gtattggcac
     3481 ggttttttca acgggtgaca cgcgtaatat ctcgcttgtc atgacggaag cagataaagc
     3541 cttacgcgaa gcgaaaagcg aggggggcaa caaagtgatt attcatcata tttaagtgca
     3601 aaaatattca gagccatgct ttttgcgtgg cttttgcata caatttatta ctattacccc
     3661 taaatttccc ctccccagcg gcgcagagat gagtataatt agcgcccctg tgccaggccg
     3721 caatcgaact ttatctggtt ttctcgtttc actaaccgaa ggagtgccat ttatcatgaa
     3781 attgcaccat agaatgctcc gg
//
LOCUS       DAJTZL010000271         3797 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.274, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000271 DAJTZL010000000
VERSION     DAJTZL010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3797
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.274"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(98..907)
                     /gene="proC"
                     /locus_tag="OO544_004016"
     CDS             complement(98..907)
                     /gene="proC"
                     /locus_tag="OO544_004016"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HCR6769483.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            1027..1485
                     /locus_tag="OO544_004017"
     CDS             1027..1485
                     /locus_tag="OO544_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HCR6769484.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            1668..2192
                     /gene="aroL"
                     /locus_tag="OO544_004018"
     CDS             1668..2192
                     /gene="aroL"
                     /locus_tag="OO544_004018"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HCR6769485.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            2242..2433
                     /gene="yaiA"
                     /locus_tag="OO544_004019"
     CDS             2242..2433
                     /gene="yaiA"
                     /locus_tag="OO544_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HCR6769486.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            2691..3368
                     /locus_tag="OO544_004020"
     CDS             2691..3368
                     /locus_tag="OO544_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HCR6769487.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLTVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            3440..3724
                     /gene="ppnP"
                     /locus_tag="OO544_004021"
     CDS             3440..3724
                     /gene="ppnP"
                     /locus_tag="OO544_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HCR6769488.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
BASE COUNT          951 a          904 c          990 g          952 t
ORIGIN      
        1 cgtgagtggt tgaaatcggc agatttggcg ctttacaaag caaagaaagc cggacgtaac
       61 cgcaccgaag tggcggcctg acgtccggcg aaagtcatca ggatttgctg agtttttctg
      121 atttttccat acacttcgtc atcgcttcga tcactgcggc acggaagcct ttctcttcca
      181 gtacgcgtac cgcttcaatg gtggtgcctc ccggtgagca gaccatatct ttcagtgccc
      241 ccggatgttc tcccgtttcc agcaccattt ttgcggaacc cattaccgcc tgagcggcaa
      301 atttatacgc ctgggcgcgt ggcatcccgc ccagcacggc ggcgtcggcc atcgcttcga
      361 taaacataaa tacgtaggct ggcgaagaac cgctcacacc gaccaccggg tggatcatcg
      421 gctcagcaat tacttccgct tcgccaaagc agcggaaaat attcagcaca tcagcggtat
      481 cttcgggggt taccagcgcg tttggcgtta cggaggtcat cccggcatta accagcgcgg
      541 gagtgttcgg catggcgcgg ataattttcc ggtcatggcc cagcgcgcgg gcaagctggt
      601 cgagcgtgac acctgcagca atagaaacga ccagagagtc tttattcagg ctggaggtga
      661 tttcgctaag cactttaatc atgatgccag gtttaacggc ggcaaaaatg atgtcggcga
      721 tttgcgccac ttcttgcgcc gattctgcgg cgttgatgcc gaactggtca tgcagggcgg
      781 cgactttatc cggggagggg gtgtataccc agatttgccc tggaagcacc tgaccgctgg
      841 caatcagacc gccgagaatg gcttttccca tattgccgca gccaataaaa ccgattttct
      901 tttccattgc ctcactcctg ccgtgaaatt cattgttttg ataatcgctg gcagaagcat
      961 aaacagaact atgccggaag gcaaaagcgc gacacaatag aggattaccc aacaaaggat
     1021 gactttatga caatttgggt ggatgccgac gcgtgtccca atgtaattaa agagattttg
     1081 tatcgcgcgg cggaacgtat gcagatgccg ctggtactgg tagcaaacca gagtttacgc
     1141 gtgccgccat cgcgatttat tcgtacgctg cgcgtcgcgg caggtttcga cgttgccgat
     1201 aacgaaattg tccggcagtg tgaagcgggc gatttggtga tcaccgcaga tatacctttg
     1261 gctgctgaag ccatcgagaa aggcgcagcg gcgcttaatc cgcgcggcga acgttacacg
     1321 ccagcgacca ttcgtgagcg cctgacgatg cgcgatttta tggatacctt acgtgccagt
     1381 gggatccaga ccggcggacc agatagcctt tcacaacgtg accgccaggc ctttgccgcg
     1441 gagctggaga agtggtggct ggaagtgcaa cgtagtcgtg gctaaatgta atttattatt
     1501 tacacttcat tcttgaatat ttattggtat agtaagggat gtattgagat tttcacttta
     1561 agtggaattt tttctttaca atcgaaattg tactagtttg atggtatgat cgctattctc
     1621 atgacaccgg ctttcgccgc attgcgacct attggggaaa acccacgatg acacaacctc
     1681 tttttctgat cgggcctcgg ggctgtggta aaacaacggt cggaatggcc cttgccgatt
     1741 cgcttaaccg tcggtttgtc gataccgatc agtggttgca atcacagctc aatatgacgg
     1801 tcgcggagat cgtcgaaagg gaagagtggg cgggatttcg cgccagagaa acggcggcgc
     1861 tggaagcggt aactgcgcca tccaccgtta tcgctacagg cggcggcatt attctgacgg
     1921 aatttaatcg tcacttcatg caaaataacg ggatcgtggt ttatttgtgt gcgccagtat
     1981 cagtcctggt taaccgactg caagctgcac cggaagaaga tttacggcca accttaacgg
     2041 gaaaaccgct gagcgaagaa gttcaggaag tgctggaaga acgcgatgcg ctatatcgcg
     2101 aagttgcgca tattatcatc gacgcaacaa acgaacccag ccaggtgatt tctgaaattc
     2161 gtagcgccct ggcacagacg atcaattgtt gattttagag cgcctatact taacgttcat
     2221 cccgtgaaat aaggaagaac gatgccaacg aaaccgcctt atcctcgtga agcatatata
     2281 gtgacgattg aaaaaggaaa gccaggacag acggtaacct ggtaccaact cagagccgat
     2341 catcctaaac cagactcgtt gatcagtgaa catccgacgg ctcaggaagc gatggatgcg
     2401 aaaaaacgct atgaggaccc tgacaaagag tgaccgcatc agactgctcg gaagggattc
     2461 tgagtgccac tacaagggat ctgcgtcaca tttttcataa ttcatgtttt tctaataatt
     2521 agaatattaa acaataacaa tccattactg gaatcatttg gaatctttac attatgccgt
     2581 gcacgtctgc tgctacgctt tttgtcattt gtagcacaag taagtgtcag cagtggtgct
     2641 ccacacttgc ccggtaatta acgacgaaag aaaagtaagg tggatgaaca atgagtgcgt
     2701 cgttggcgat cctgaccatc ggcattgtac ctatgcagga ggttttgccg ctcctgacgg
     2761 aatacattga cgaagataat atttcccatc atagcctgct ggggaagtta agtcgtgaag
     2821 aagtgatggc ggagtacgcg ccagaagcag gcgaagacac cattctcaca ttattaaatg
     2881 acaaccagct ggcccatgtt tcgcgtcgta aagtggagcg agacctgcaa ggtgtggttg
     2941 aagtgctcga taatcagggt tatgacgtca ttttattaat gagtacagca aacattagta
     3001 gtatgactgc gcgtaatacg atctttcttg agccgtcgcg aatattgcct ccactggttt
     3061 cctctattgt tgaagatcat caggtggggg ttatcgttcc ggttgaggag atgctgaccg
     3121 ttcaggcgca aaaatggcaa attttgcaga aatcgccggt attttcattg ggtaacccca
     3181 ttcatgattc agaacaaaaa atcattgatg ccgggaaaga attactggca aaaggggctg
     3241 atgtcatcat gctggattgt ttaggatttc accaacgtca tcgcgattta ctgcaaaaac
     3301 agctcgatgt tcctgtcttg ctgtctaacg tattgattgc acggctggct gcggaattac
     3361 tggtgtaatt ttgcgtgaca gccagcgcct ctggccccta tagtgaagta gatgttcaac
     3421 taccagacag ggccagttta tgcttcaaag taatgagtac ttttccggca aagtgaaatc
     3481 aatcggcttt tccagcagca gcactggtcg cgccagcgtg ggtgttatgg ttgaaggcga
     3541 atacaccttc agcaccgctg agccggaaga gatgacggta atcagtggcg cgctgaatgt
     3601 gttactgcct gacgcgaccg actggcaggt gtatgaagcc ggttcggtgt ttaatgttcc
     3661 cggtcacagt gagtttcatc tgcaagttgc cgaacccacc tcttatctgt gccgctatct
     3721 gtaattcctc gccttcccct tgaacgggag ggcatttttc tgaaatatcc tctctttaac
     3781 ccataataat atttcct
//
LOCUS       DAJTZL010000272         3734 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.275, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000272 DAJTZL010000000
VERSION     DAJTZL010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3734
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.275"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..181)
                     /locus_tag="OO544_004022"
     CDS             complement(<1..181)
                     /locus_tag="OO544_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel protein MscS"
                     /protein_id="HCR6769489.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSI"
     gene            425..682
                     /locus_tag="OO544_004023"
     CDS             425..682
                     /locus_tag="OO544_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HCR6769490.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            complement(732..1682)
                     /gene="uspE"
                     /locus_tag="OO544_004024"
     CDS             complement(732..1682)
                     /gene="uspE"
                     /locus_tag="OO544_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HCR6769491.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(1834..2586)
                     /gene="fnr"
                     /locus_tag="OO544_004025"
     CDS             complement(1834..2586)
                     /gene="fnr"
                     /locus_tag="OO544_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HCR6769492.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(2781..3296)
                     /gene="ogt"
                     /locus_tag="OO544_004026"
     CDS             complement(2781..3296)
                     /gene="ogt"
                     /locus_tag="OO544_004026"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HCR6769493.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLRDYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(3307..>3734)
                     /locus_tag="OO544_004027"
     CDS             complement(3307..>3734)
                     /locus_tag="OO544_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HCR6769494.1"
                     /translation="FIAVGLTDILESSGLSGIPAFVGLALLSSFLCMFIASGSAIWSI
                     LAPIFVPMFMLLGFHPAFAQILFRIADSSVLPLAPVSPFVPLFLGFLQRYKPDAKLGT
                     YYSLVLPYPLIFLVVWLLMLLAWYLVGLPIGPGIYPRLS"
BASE COUNT         1002 a          886 c          893 g          953 t
ORIGIN      
        1 taatgctacc aacagcactg ataattatgg tgtagattaa aaattgcacc gcatggaata
       61 aaaaaccttt tcttttacga tttccgcggc gaaaccaaaa gctcatcaga atcaatgctg
      121 cgcagctacc gaaaataatg accagattaa gcgcattatt tgtaaacagt tcagcgatca
      181 ttgttttatc aggctcctcc agataattgt cgtcatgccg gaaacccctg gcggggctat
      241 tttaccgcga caattcattc agatcatcaa tagtcaggga aggaagtagc aacattagct
      301 aactaatcaa tgcgttgatt gtaaatccag ctaagaggtg aggttttcag agcagacaac
      361 ggtgaaatgt catggtattg ttacgtttag gtaacaagaa atttgtctgc acaaggatta
      421 catcatgatt atggcgaaac tgaagtcagc gaaagggaaa aaatttctct ttggtttgtt
      481 ggcggttttc attattgcgg cgtcggttgt gactcgcgcg actatcggcg gcgttataga
      541 acagtacaat attccgctgt ctgagtggac gacatcaatg tatgtgattc agtcatcgat
      601 gatttttgtt tatagcctgg tctttactgt gttgctggca atcccgttgg gaatttattt
      661 ccttggcggc gaagagcagt aagtaaaaaa taggcccgat aactcgggcc ttgtcagtta
      721 ttgaagagtc gttaatcgtc ttcttcgtca tccagttcaa cgggtgtctg atactggtca
      781 ggtttaatga ccaacagatc gcagcgaaga tgatcaatca cctgttccgc cgtgttgccg
      841 aggaatgctg ctgaaatacc ggtgcgtcct accgtgccca gaaccacaat ccccgcctgt
      901 aagtgctccg ccaaatcagg aataacctct tccggcagac ctttttctac gtgcgtcatg
      961 ttttcattaa tgccgaattt ctgccgcaga gctttcattg ccagcaaatg ttgcccacga
     1021 atggcatcgt tataaacgct cggatcaaat tccggcagtt caatcgcgat attaattggc
     1081 gttactgggt aagcgccaac cagatgaact tcggtatggt tgacttgttc tgccagttcg
     1141 atcgtctctt tgaccagttt ttcattgagc gcattatgat acggctcttc actggcgaga
     1201 ttcaccgcca ccagcgcctt acctccttcc ggccacggct ggtctttcac catccacacc
     1261 gggcttgggc atttgcgtaa cagatgccag tccgttggcg taaaaatcac cgcttccaga
     1321 cggtcatgtt ggtgcgccat ttttagcacc aaatcgtgcc cgccgctgat cacttcctga
     1381 atgatggctt cgaagggacg gttatgccag accactttaa tttcaatggg aacgccagca
     1441 ttgagataat attttgcctg ctcgtggatc caggctgtac gctggctgat gacgccctga
     1501 cgcatagcgg tacgttcgtc cggggagagc agggtggtca tttcgtatga gaagtcatag
     1561 atcggcaaaa aggctttaat tttgccacca atccgttgat gtaaataaac agctcgccgc
     1621 aatgctggtt ggtcgtcctg gttaggatcg ataacaacga gcatgttctg atacatagcc
     1681 atacagggtc tccttacaac aactgtcaac gcagtttgta attaaaagat taacccatat
     1741 ctggtgaatg aaacagtgat gaaccttctg ccagatcaat aaatcagaaa aatttaatga
     1801 tatgacagaa ggatagtgag ttatgcggaa aaatcaggca acattacgcg tatgaccagc
     1861 aagctgggcc agcgcatcgt tattttcgat ggtgatgtat ttacctttga ctgccagcat
     1921 accgcttttc tggaagcgac ctagcagacg gctgatggtt tctaccgtca ggcccagata
     1981 gttaccgata tcgccacgag tcatcgtcag gcggaattca cgaggggaga agccgcgttg
     2041 ggcaaaacga cgggacaggt tgtagatgaa tgcagccaga cgttcctcgg catttttctt
     2101 cgacaacagc aagatcatgt cctgatcgcc tttgatttca ccgctcatca gacgcatcat
     2161 ctgctgacgc agattcggca ttttaccgga caaatcgtcc agcgtttcga acgggatttc
     2221 acataccatc gaggtttcca gcgcctgagc gaagctcggg tgatggccgc tgccgatggc
     2281 gtcaaatccc accaggtcgc cagctaaatg gaaaccagtg atttgctcgt cgccttgctc
     2341 agtgatggta taacttttaa tcgtaccgga gcggatggca taaagcgatt taagttcatc
     2401 accagcctta aacagcgtct ggcctttctg aataggcttc ttccgctcaa tgatattatc
     2461 aagctgatca agctcatgtt cgttgagtgt gaacgggatg caaagctggc tgatgctgca
     2521 atcctggcaa tggatagcac aaccgccaga ctgaatgcgc cgtataattc gcttttccgg
     2581 gatcataggt ctgctcaagc cgtaattgat atttgtcaat tttaacatct ttttagggag
     2641 caagtaagtc taagcaaacc ttaacagcag agaattccga tattagatgt aaatatatgt
     2701 ctatctattt gaaaaccctt aagttgttaa gggtaacttt acataaaagt gtgaacaagc
     2761 tggcacaaat tgtttaatgt ttacagcaaa agataacctt cgtggcgcaa taaccactct
     2821 tttcgctgaa ctccgcctgc atatccggtc atggtgccgt ttcggccaat aacccgatgg
     2881 caaggtacga cgatgctgat gggattcgat ccgtttgccg caccaacggc acgcgccgcg
     2941 ccagggcggc ccaattgttc agccagttgg ccgtaatgca ttacctgtcc gcaggggata
     3001 gtgcgtagag ttttccagac ttcgcgctga aatggcgtcc cccctgtggc agtgggaagc
     3061 gtatcaataa tgctaagatt accggcaaaa taatcacgaa gcttgtcgct taaaccgcct
     3121 gggttggtgg cagaaatgcg ctcatagcct tctttgcgat aatggatgtc cagcagctgc
     3181 accatgcgtt cgctgtactc ttcccattca accgcccgca ggcgaaattg ctcatcgcaa
     3241 atcacccaca gtggacccag tggcgtggca attttttctt caagtaatct cagcatccgt
     3301 tctctcttaa gacaaacgtg ggtaaatccc cggacctatc ggcagaccaa caagatacca
     3361 cgccagcaac atcagcagcc ataccaccaa aaagataagc ggatagggca agactaacga
     3421 atagtaagta cccagtttcg cgtctggttt gtagcgttgc aggaatccaa gaaacagtgg
     3481 aacaaaagga gataccggcg ctaaaggcaa tacggatgag tcggcaatac gaaagaggat
     3541 ttgcgcaaat gccgggtgaa agccaagtag cataaacatt ggtacgaaaa tgggggccag
     3601 aatcgaccag attgcggaac cgctggcaat aaacatgcat aagaaagagg aaagcaacgc
     3661 cagaccgaca aacgccggga tgccgctaag ccctgaactt tccagtatat cggtcagccc
     3721 cacggcgatg aatt
//
LOCUS       DAJTZL010000273         3730 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.276, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000273 DAJTZL010000000
VERSION     DAJTZL010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3730
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.276"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            65..172
                     /locus_tag="OO544_004028"
     CDS             65..172
                     /locus_tag="OO544_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HCR6769495.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(259..1938)
                     /gene="bcsG"
                     /locus_tag="OO544_004029"
     CDS             complement(259..1938)
                     /gene="bcsG"
                     /locus_tag="OO544_004029"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HCR6769496.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFATFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGASVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(1935..2126)
                     /gene="bcsF"
                     /locus_tag="OO544_004030"
     CDS             complement(1935..2126)
                     /gene="bcsF"
                     /locus_tag="OO544_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HCR6769497.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHFLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(2123..3694)
                     /gene="bcsE"
                     /locus_tag="OO544_004031"
     CDS             complement(2123..3694)
                     /gene="bcsE"
                     /locus_tag="OO544_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HCR6769498.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
BASE COUNT          956 a          937 c          950 g          887 t
ORIGIN      
        1 aatcttgaac ccaaacaact caagaatagc cgcgaaacgt tggcattaca acacaggcgg
       61 ctatatgacg ctcgcagaac tgggcatggc cttctggcat gatttagcgg ctccggtcat
      121 tgctggcatt cttgccagta tgatcgtgaa ctggctgaat aagcggaagt aacgtgtcat
      181 gcgggcgtca ggctgccgta atggcaattt gcgcccggat caggccgcag aggttaaact
      241 ctgcggcctt tttcgtgctt actgcgggta aggcacccag tcgccgccgt tcaggcgaac
      301 gtacggttta tcctggtatt gaataactac tgcatttgag ttctcggaga ccggtgctgt
      361 ttgtggcaac ccactggtga gttttttcca gtcaacattg tcttcggtga aaatcttgcc
      421 atcaagaacg cgaaccacca gatcggagat agctaggaag ctgctcggtt ggtcaatgac
      481 aatcggtgcc ccctgatgtg gtgccttcat gccgaagaat ttcaccccaa cggggacgtc
      541 tgtgatagac gggctaggga tatcacgtag gccagatacc tgcattctgt cgcccttcag
      601 cgcgccgccg tgttccggca ccacgaccac catcacttta cgacccgatt tctccagttc
      661 agtaaagaag gcgtccagtt catcaaagaa tttctgcgcc cgcgctttgt aatccgctgt
      721 tttgctgacc cccggataat ggttgccgtc atgcagtgga agcgtgttgt agaacgtggc
      781 actacggctg tttttatctt tttcggtaac gtccagccag cggttaagca cggcggtatc
      841 gtcataaacc ggcgaaccat caaagcccag caaaataacc ggcagatttg tttgatccat
      901 caattcagtc tgcatgccgc cattttcgcg aacttctttc aaaaaaccgc cgaactggcc
      961 gttatgtccc atcatcaggt gctgggtaaa gcccagtttc gaaaggttat caaacagata
     1021 gcagtcgtta tttgccggtt gatacagatt agtgtgcgaa gtctgcccgc agctggcgcg
     1081 cagtaaacgg atcgccgccg ggccactgta ggaggtggcg gagttaaagt tcttgaactc
     1141 aatatcgaaa tgcgaccaca gtggatgcga catcaacccg gcggcttcta tatccgacca
     1201 ggaaagcgaa cagatgttaa tcaccagtag ttcaaatggc tgagcatcag cgggcagcga
     1261 agacgggaag gtcgatttac gtttcgcctc cgcgttatag aaattattca gccaggcgtt
     1321 aaggttcgcc gttgttggcg gtgcagtttg tgcgggcata tcacccacta ccgatgcgcc
     1381 acccgtcgcc gcaacggttg ccgctgcgtt accacccgtc gttgttacag tggtcgtcgg
     1441 ttgtccggct ggccacaagg agaaacttgg tcccgccagg gtaagtacgt tcagccatag
     1501 cagtatggca accacaaaaa cggtaatgcg aatccattgt gacaggaata accaggccac
     1561 taataaaaca aaaatggccc caatcatctg ccagttaata aagcgtgtga caaggtcgat
     1621 taaataatcg gtactgaacc ccgccacctg cgaaccctgg ctcattatgc tttccgggcc
     1681 aggcaaccag gtgtcatgcc agaacaaagc aaagccgatc ggcagggcaa tccagtggcg
     1741 caagcgatgc aggctgtagc gcggaatggg catcagcaga aacgtggcaa acaccaaatt
     1801 gaggagcgga tggaagttaa gatatcccgc ccacaacagg ccgaacttaa ccagaaaata
     1861 gaagttccag ccggaaaggc cgcgccagta ttgccagagg gaagaaggca tggcggtatt
     1921 ttgcgtaaat tgagtcattt tttggttgcc ctggcttttt ccgtgcggcg taacgtcccg
     1981 gccggtttaa cataacgagg tttagcaaag aataaacgta aggtgtcgcg aatgcgtcgc
     2041 aaaaagtgcc gcgccagata gcccagcggg aaaaatatca gtgcgcaaac gacaataatt
     2101 tcaatgatat cgctgatggt catcatgatg agcgctccac agcatcatct aacagtcgca
     2161 tgggttctgg tattcgtcgc cagtggcgac catcgtgctc ggcattgatg accggtttag
     2221 aactttgcgt taaaggcagc ggcatgcccc attgttctgg ggcaagcaag cgcatctgca
     2281 ccagctcggc actgatttga tcatcttcaa accagaccat acggtttgag aaaatgtcgc
     2341 cagtaggcaa tgggaaaata tgattcaacg cggtatccag atcgttaatc cgacagaatg
     2401 agagaaacag caccagccga ttaccgccaa tggtcatgat atcgccggta cggttagggc
     2461 gacacagcgt cagggcttgt tcaacgcgga tacccggtac cggacgtagg gcaaccagaa
     2521 cgcctttacc gtgggcaggt aatagagggt tattcatcat gttgttgacg gcattacaga
     2581 acacatccca cttctggaaa ccacgcagtt tgagcggctg ggtcattgac agcaaggtag
     2641 tgatatcttc cggcacatag cgactaaact tctgcccttg cacgctttcg atcatcgtca
     2701 gacaacggga gagtggcgca ttccacggaa taaccatatt tgcaccgcag gccaataaca
     2761 aacgttcatc ggtggcgcgc aggctagcgg tattttcccg cacgaggatt ttcatcgcac
     2821 taccgcgctg gcgacgcagg gtatgaatgc tgcgggccag tggctcgatt tgcgcatttt
     2881 gctgtaaaga aaagaccacc gtcgccgcct gagcggtacg ggcttcattg aacaggactt
     2941 cgttattgtt gaacagttgc cagtgttccg atagcggcgg cgcaccttcc agtacagcaa
     3001 cattactcag aatgcgtttt tcgtcgctgc gtggttggat ctccgcctct tcgctttgaa
     3061 ctaatgtcca gataccattt tgttgctgaa cgctaagctg ctgacgggcg ctgacccctt
     3121 tttcgttgca ccagaaggca atatccagca aatgttggtc accctgaaaa cgcaaactgg
     3181 caagaccaaa aagtgaacgg tattcctcaa gcaacaatga aaattgttta tcgttattat
     3241 ttccgggatt aattaccaaa agcgaacaat ggtttaacct gctccattta ttcattttat
     3301 ccaaccatga gcgaagccgc tcggcaggaa tgttttgcca tgcgttattt gcgcaaacaa
     3361 gaataaaaag gtaattatgg ggatcaatag aacactgcaa atcacgggcc aaatagtata
     3421 gacctttttc atgatttagc attgaaaata attttatttt ttccggccct tgagaatcat
     3481 ctaattgaaa gattttcgcc ggatcgctgt ccatgctaat gaccgcgacg tgtgcggttt
     3541 cagcctggga tgcaattgtt tgattcgcca gactgatagc atcttcatgg cgatcgacgt
     3601 taaaccacca gacgccgcct gctggcatat gtcgcagctc atcccataat gatgagatac
     3661 cgatagagaa tacagggtcc acaatgtccc tcatgtcacc attttgtctg attatcagtt
     3721 tactagcgaa
//
LOCUS       DAJTZL010000274         3684 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.277, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000274 DAJTZL010000000
VERSION     DAJTZL010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3684
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.277"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..1327
                     /locus_tag="OO544_004032"
     CDS             <1..1327
                     /locus_tag="OO544_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TniQ family protein"
                     /protein_id="HCR6769499.1"
                     /translation="QQLIYEHTLFPLYAPFVGKERRDEAIRLMEYQAQGAVHLMLGVA
                     ASRVKSDNRFRYCPDCVALQLNRYGEAFWQRDWYLPALPYCPKHGALVFFDRAVDDHR
                     HQFWALGHTELLSDYPKDSLSQLTALAAYIAPLLDAPRAQELSPSLEQWTLFYQRLAQ
                     DLGLTKSKHIRHDLVAERVRQTFSDEALEKLDLKLAENKDTCWLKSIFRKHRKAFSYL
                     QHSIVWQALLPKLTVIEALQQASALTEHSITTRPVSQSVQPNSEDLSVKHKDWQQLVH
                     KYQGIKAARQSLEGGVLYAWLYRHDRDWLVHWNQQHQQERLAPAPRVDWNQRDRIAVR
                     QLLRIIKRLDSSLDHPRATSSWLLKQTPNGTSLAKNLQKLPLVALCLKRYSESVEDYQ
                     IRRISQAFIKLKQEDVELRRWRLLRSATLSKERITEEAQRFLEMVYGEE"
     gene            1328..2944
                     /locus_tag="OO544_004033"
     CDS             1328..2944
                     /locus_tag="OO544_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator"
                     /protein_id="HCR6769500.1"
                     /translation="MVRLATFNDNVQVVHIGHLFRNSGHKEWRIFVWFNPMQERKWTR
                     FTHLPLLSRAKVVNSTTKQINKADRVIEFEASDLQRAKIIDFPNLSSFASVRNKDGAQ
                     SSFIYEAETPYSKTRYHIPQLELARSLFLINSYFCRSCLSSTALQQEFDVQYEVERDH
                     LEIRILPSSSFPKGALEQSAVVQLLVWLFSDQDVMDSYESIFRHYQQNREIKNGVESW
                     CFSFDPPPMQGWKLHVKGRSSNEDKDYLVEEIVGLEINAMLPSTTAISHASFQEKEAG
                     DGSTQHIAVSTESVVDDEHLQLDDEETANIDTDTRVIEAEPTWISFSRPSRIEKSRRA
                     RKSSQTILEKEEATTSENSNLVSTDEPHLGGVLAAADVGGKQDATNYNSIFANRFAAF
                     DELLSILKTKFACRVLFEETLVLPKVGRSRLHLCKDGSPRVIKAVGVQRNGSEFVLLE
                     VDASDGVKMLSTKVLSGVDSETWRNDFEKIRRGVVKSSLNWPNSLFDQLYGQDGHRGV
                     NHPKGLGELQVSREDMEGWAERVVREQFTH"
     gene            complement(3000..3152)
                     /locus_tag="OO544_004034"
                     /pseudo
     CDS             complement(3000..3152)
                     /locus_tag="OO544_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011069623.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(3175..3552)
                     /locus_tag="OO544_004035"
     CDS             complement(3175..3552)
                     /locus_tag="OO544_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6769501.1"
                     /translation="MSAAKALAYALCDKLESKKVDEVVRNLVSSGWNIREVAWEASSF
                     DHEMPYFDTGRDFDIGFPDTLSSKIEFLTANGSIVQARARLHFKQALIFAKASKHVAA
                     LKNSLDYYYGSSVIPPQNHGGYL"
BASE COUNT         1061 a          712 c          921 g          990 t
ORIGIN      
        1 tcagcagctt atttatgagc ataccttatt ccctttatat gctccgtttg taggcaagga
       61 gcgccgagac gaagctattc ggttaatgga gtaccaagcg caaggtgcgg tgcatttaat
      121 gctaggagtc gctgcttcta gagttaagag cgataaccgc tttagatact gccctgattg
      181 cgttgctctt cagctaaata ggtatgggga agccttttgg caacgagatt ggtatttgcc
      241 cgctttgcca tattgtccaa aacacggtgc tttagtcttc tttgatagag ctgtagatga
      301 tcaccgacat caattttggg ctttgggtca tactgagctg ctttcagact accccaaaga
      361 ctccctatct caattaacag cactagctgc ttatatagcc cctctgttag atgctccacg
      421 agcgcaagag ctttccccaa gccttgagca gtggacgctg ttttatcagc gcttagcgca
      481 ggatctaggg ctaaccaaaa gcaagcacat tcgtcatgac ttggtggcgg agagagtgag
      541 gcagactttt agtgatgagg cactagagaa actggattta aagttggcag agaacaagga
      601 cacgtgttgg ctgaaaagta tattccgtaa gcatagaaaa gcctttagtt atttacagca
      661 tagtattgtg tggcaagcct tattgccaaa actaacggtt atagaagcgc tacagcaggc
      721 aagtgctctt actgagcact ctataacgac aagacctgtt agccagtctg tgcaacctaa
      781 ctctgaagat ttatctgtta agcataaaga ctggcagcaa ctagtgcata aataccaagg
      841 aattaaggcg gcaagacagt ctttagaggg tggggtgcta tacgcttggc tttaccgaca
      901 tgacagggat tggctagttc actggaatca acagcatcaa caagagcgtc tggcacccgc
      961 ccctagagtt gattggaacc aaagagatcg aattgctgta cgacaactat taagaatcat
     1021 aaagcgtcta gatagtagcc ttgatcaccc aagagcgaca tcgagctggc tgttaaagca
     1081 aactcctaac ggaacctctc ttgcaaaaaa tctacagaaa ctgcctttgg tagcgctttg
     1141 cttaaagcgt tactcagaga gtgtggaaga ttatcaaatt agacggatta gccaagcttt
     1201 tattaagctt aaacaggaag atgttgagct taggcgctgg cgattattaa gaagtgcaac
     1261 gttatctaaa gagcggataa ctgaggaagc acaaagattc ttggaaatgg tttatgggga
     1321 agagtgagtg gttaggctag ctacatttaa tgacaatgtg caggttgtac atattggtca
     1381 tttattccgt aactcgggtc ataaggagtg gcgtattttt gtttggttta atccaatgca
     1441 agaacggaaa tggactcgat ttactcattt gcctttatta agtcgagcta aggtggttaa
     1501 cagtacaaca aagcaaataa ataaggcgga tcgtgtgatt gagtttgaag catcggatct
     1561 tcaacgagcc aaaataatcg attttcctaa tctctcgtcc tttgcttccg tacgcaacaa
     1621 ggatggagcg cagagttcat ttatttacga agctgaaaca ccatatagca agactcgtta
     1681 tcacatccca cagttagagc tagctcggtc attattttta attaactcct atttctgtcg
     1741 aagctgtttg agcagtaccg ctttacagca agagttcgac gttcagtatg aggttgagcg
     1801 agatcattta gagataagga tcttacccag ttcatcgttt cctaaagggg cgttagagca
     1861 gtcggccgta gtgcagcttt tggtttggtt gttttcggat caagatgtta tggattcgta
     1921 tgaaagtatt tttaggcact atcaacaaaa tagagaaatt aagaacggcg ttgaaagctg
     1981 gtgctttagc tttgaccctc cgcccatgca gggttggaaa ttacatgtaa aaggacgttc
     2041 ttctaacgag gataaggatt atttagttga ggaaatagta ggtttagaaa tcaacgctat
     2101 gcttcctagc acaacagcta ttagccatgc ctcttttcag gaaaaggagg caggtgatgg
     2161 tagtacgcag cacatagcgg tttcaacaga gtcagttgtt gatgatgagc atctacagtt
     2221 ggacgatgag gaaacagcca atatagacac agacacacga gtcatagagg ctgagccgac
     2281 atggataagt tttagtagac ctagtcgaat tgaaaaatct cgcagggcaa gaaaaagtag
     2341 ccaaactatt ttagaaaaag aagaagcaac aacaagtgaa aatagtaatt tggttagtac
     2401 tgatgagcca cacttaggtg gtgtcctagc agcggcagat gtgggtggga agcaggatgc
     2461 aaccaattac aactctattt ttgctaatcg atttgctgct tttgatgagc tactttcaat
     2521 tctaaaaact aaatttgcat gtcgggtgct ttttgaagaa accttggttt tgccaaaagt
     2581 tgggcgtagc cgattacatc tgtgtaaaga tggctcacca agagtgatta aagccgttgg
     2641 ggtgcaacgt aatggcagtg aatttgtatt gctagaggtg gatgcatcgg atggggtgaa
     2701 aatgctttct accaaagtgt tgagtggcgt tgatagcgaa acatggcgga atgattttga
     2761 aaagatacgg cgtggagtgg tgaagagctc attgaattgg ccaaatagtt tgtttgatca
     2821 attatatgga caagacgggc atagaggggt gaatcatcca aaggggttgg gggagctgca
     2881 agtatcgaga gaggatatgg aagggtgggc tgagagagtg gttagagagc aatttacgca
     2941 ttaaaggaat gactgaaaga gcctgtaaac ccttttgtgt aagtgctttt gccggtcagt
     3001 taaaggtggc catttaaacg gtcaccaaat tcgatcataa aacggttcat ggccggcttc
     3061 cagttgcgga tcggcatcgt ccatttcttg gtcgccgcct ggatagccag gtacaccacc
     3121 ttcatcgctg attcgtccgt agggaacacc ttgagttcat attaatggaa ttttctacaa
     3181 atagcctccg tggttttgag gggggattac agacgatcca tagtagtaat ccaatgagtt
     3241 cttgagcgcg gcgacatgtt tggacgcctt ggcaaaaatt agagcctgct tgaagtgcag
     3301 gcgagcacgt gcttggacga ttgatccatt cgcggtcaaa aactcaatct tggatgacag
     3361 cgtgtcagga aatccaatat cgaagtcccg ccccgtgtca aagtagggca tttcatgatc
     3421 aaaggacgaa gcttcccagg ccacttccct aatattccag ccgctagaaa caagatttcg
     3481 tacgacttca tcaacttttt tgctttccaa cttgtcacaa agtgcatagg caagagcttt
     3541 ggcggcagac acgtgcgttt cctcttaaac ataggtcgaa cggatacccg aggcaaagcg
     3601 gagcattagt cccaaagcat ctccggtaga tccggaggct ccatactgaa ggcatggcat
     3661 cttgggcatt gatatttgaa actt
//
LOCUS       DAJTZL010000275         3610 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.278, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000275 DAJTZL010000000
VERSION     DAJTZL010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3610
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.278"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..71)
                     /locus_tag="OO544_004036"
     CDS             complement(<1..71)
                     /locus_tag="OO544_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001605275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6769502.1"
                     /translation="MPVPNPAIPVKGAGTTLWVYNGSG"
     gene            complement(79..474)
                     /locus_tag="OO544_004037"
     CDS             complement(79..474)
                     /locus_tag="OO544_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6769503.1"
                     /translation="MKHTELRAAVLDALEKHDTGATFFDGRPAVFDEADFPAVAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDAWMESRIYPVMSDIPALSDLITSM
                     VASGYDYRRDDDAGLWSSADLTYVITYEM"
     gene            complement(471..1049)
                     /locus_tag="OO544_004038"
     CDS             complement(471..1049)
                     /locus_tag="OO544_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6769504.1"
                     /translation="MAIKGLEQAVENLSRISRTAVPGAAAMAINRVASSAISQSASQV
                     ARETKVRRKLVKERARLKRATVKNPQARIKVNRGDLPVIKLGNARIVLSRRRRRKKGQ
                     RSALKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            complement(1061..1414)
                     /locus_tag="OO544_004039"
     CDS             complement(1061..1414)
                     /locus_tag="OO544_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HCR6769505.1"
                     /translation="MADFDNLFDAAIARADETIRGYMGTSATMTSGEQSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDDVRQLRRGDTLTIGEENFWIDRISPDDGGSCH
                     LWLGRGVPPAVNRRR"
     gene            complement(1426..1821)
                     /locus_tag="OO544_004040"
     CDS             complement(1426..1821)
                     /locus_tag="OO544_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HCR6769506.1"
                     /translation="MTKDELIARLQVLGEQLNRDVSLTGTKEELALRVAELEEELDDT
                     DDAAGQDTSVSPENALTGHENEVVSAQPDTVTDTADLVTVVALVTLHTDALHATRDEA
                     VAFVLPGTAFRVSAGVAAEMTERGLARMQ"
     gene            complement(1863..2888)
                     /locus_tag="OO544_004041"
     CDS             complement(1863..2888)
                     /locus_tag="OO544_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HCR6769507.1"
                     /translation="MSMYTTAQLLAANEKKFKFDPLFLRLFFRESYPFTTEKVYLSQI
                     PGLVNMALYVSPIVSGEVIRSRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLA
                     DPAYRRRRIIMQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSAAN
                     NITQSGGTEWSKRDKSTYDPTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVKEKL
                     DTRRGSNSELETAVKDLGEAVSYKGMYGDTAIVVYSGQYVENDVKKNFLPDNTMVLGN
                     TQARGLRTYGCIQDADAQREGINASARYPKNWVTTGDPAREFTMIQSAPLMLLADPDA
                     FVSVQLA"
     gene            complement(2944..3276)
                     /locus_tag="OO544_004042"
     CDS             complement(2944..3276)
                     /locus_tag="OO544_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HCR6769508.1"
                     /translation="MTSKETFTHYQPLGNSDPAHTATAPGGLSAKAPAMTPLMLDTST
                     RKLVAWDGTTDGAAVGILAVDADQTSTTLTFYKSGTFRYEDVLWPEAASDETKKRTAF
                     AGTAISIV"
     gene            complement(3286..>3610)
                     /locus_tag="OO544_004043"
     CDS             complement(3286..>3610)
                     /locus_tag="OO544_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6769509.1"
                     /translation="ATEGENASAAQPDVNAQITAAVAAENSRIMGILNCEEAHGREEQ
                     ARVLAETPGMTVETARRILAVAPQSAQARSDTALDRLMQGAPAPLAAGNPASDAVNDL
                     LNTPV"
BASE COUNT          742 a         1116 c          946 g          806 t
ORIGIN      
        1 ccgctcccgt tataaaccca cagggtggtt ccggcacctt tcaccggtat tgctggattt
       61 ggtacaggca tatcgtcctc acatttcata ggtaatgaca taagtcagat cggcagaact
      121 ccacaggccc gcatcatcgt cgcgccggta gtcatagcca ctggccacca tactggtgat
      181 caaatctgac agtgccggga tatcgctcat caccggataa atccgggact ccatccacgc
      241 atccagctct gaatccggca cctgagcagg caggaaaact tcgatatgca gttccgcctg
      301 ccaggtatcg ctgtccagct cttcgcccgt gtattcagcg ccggtgagat aaacggcaac
      361 tgccggaaaa tccgcctcat caaaaacagc ggggcgacca tcaaaaaacg tcgccccggt
      421 gtcatgcttc tccagtgcat ccagtacggc tgcacggagt tcagtatgtt tcatcgcttt
      481 attaccatcc tcagttgatg ctgcagcgca tagcccagct ctttcggaag acgctcacgc
      541 cgtatccgct caatattctg tttaaacgcc gtggtcagcg gcaccgccat cgggattttc
      601 accacatcaa tggggtaacg gtttttcccg gccacacgct gcatgacatg ccagcggcca
      661 tttttcagtt gctgaataaa cgcgccggga atacgacggt ttcccaccac aagcacgctg
      721 ccgccacctt tcagggctga acgctgcccc tttttacgac gcctgcgtcg ggacaggaca
      781 atccgcgcgt tacccagctt tattacgggc aaatcccccc ggttaacctt gattctggcc
      841 tgcggatttt tgaccgtggc ccttttcagc ctggcccttt cctttaccag tttccggcgt
      901 acctttgtct cacgggcaac ctgtgacgcc gactgcgata tcgcggatga agcaacgcgg
      961 ttaatggcca ttgcggcggc accgggcacc gccgttctgc tgatacggct gaggttttca
     1021 acggcctgct caagaccttt tatggccata catccccctt tcagcggcga cggttaacgg
     1081 caggcggtac gccccgccca agccagagat gacagcttcc gccatcatcc ggcgaaatcc
     1141 ggtctatcca gaagttttcc tcaccgatgg tcagcgtgtc gccgcgccgc agctgccgca
     1201 catcatcagt ccggacaaac agggacgggc tggagccttc aacgcgcacg ccctgtccgg
     1261 catagctgat attttcaggg tcatcaaaaa caccacgtat cacagcaccg gactgctcac
     1321 cggatgtcat ggtggctgac gttcccatgt acccgcgtat cgtttcatcg gcgcgggcaa
     1381 tggcagcatc gaacaggtta tcgaaatcag ccacagcgcc tcccgttatt gcattctggc
     1441 caggccgcgc tctgtcattt cagctgccac accggcagag acacggaacg ccgttcccgg
     1501 cagcacaaat gccacagcct catcccgcgt ggcgtgaagt gcatcagtat gcagcgtcac
     1561 cagtgccaca accgtgacca gatcagccgt atcagtcacg gtatccggct gcgctgatac
     1621 aacctcattt tcatgtccgg tcagcgcatt ttccgggctg acagatgtgt cctgaccagc
     1681 agcgtcatcc gtgtcatcaa gctcctcttc cagctctgcc acacggagtg ccagttcttc
     1741 tttcgtcccc gtcaggctga catcacggtt cagttgctca cccagcacct gaagacgggc
     1801 aatcagttca tctttcgtca tggactcctc cacagagaga aaatggcccc gaagggccat
     1861 gattacgcca gttgtacgga cacgaacgca tcagggtcag ccagcagcat cagcggtgct
     1921 gactgaatca tggtgaactc acgcgccgga tcgccggtgg tcacccagtt tttcgggtag
     1981 cgggcagagg cgttaatacc ttcgcgctgt gcgtccgcat cctgaatgca gccataggtg
     2041 cgcagaccgc gtgcctgagt gttccccagc accatcgtgt tgtccggaag gaagttcttt
     2101 ttgacgtcgt tttccacgta ctgtccggaa tacacgacga tcgccgtatc gccatacatc
     2161 cccttatagg acaccgcttc gcccaggtct tttaccgctg tctccagctc ggaattagag
     2221 ccgcgacggg tatccagctt ctccttgacg gctttgaagg aacggaacag cgcccagcct
     2281 ttcggatcaa acacgatgat attcaccact ccgctggcgt tcagcgcata ggcttcgata
     2341 tcgtcggtcg ggtcatacgt ggacttgtca cgcttgctcc actccgtacc accggactgt
     2401 gtgatgttgt tcgccgcact gcggcccata tccacttcaa ccggatcgaa ggcttcaccg
     2461 gtcatggtat atttgccctt aagcacggca gaaacggcct gcatctcttc gacctgagca
     2521 atggccagct cttcgtctcg catgttctgc atgatgatgc gacggcggcg gtaagccggg
     2581 tccgccagat tctgcggatc ttcatccggc aggcgacgca gggtcatctg cggattcacc
     2641 tcatgcttcg gcttgacata tcccggtgta aattcagagg tggagccgcc acgggagcgg
     2701 ataacctcac cggaaacaat cggcgaaacg tacagcgcca tgtttaccag tcccggaatt
     2761 tgtgagagat agactttctc cgtggtgaag ggatagctct cacggaaaaa gagacgcaga
     2821 aacagcggat caaacttaaa tttcttctca tttgccgcca gcagctgggc ggttgtgtac
     2881 atcgacataa aaaaatcccg taaaaaaagc cgcacaggcg gcctttagtg atgaagggta
     2941 aggttaaacg atgctgattg ccgttccggc aaacgcggtc cgtttcttcg tctcgtcgct
     3001 ggcagcctcc ggccagagca catcctcata acggaacgtg ccggacttgt agaacgtcag
     3061 cgtggtgctg gtctggtcag catcaaccgc cagaatgcca acggcagcac cgtcggtggt
     3121 gccatcccac gcaaccagct tacgggtgga ggtatccagc atcagcgggg tcattgcagg
     3181 cgctttcgca ctcaatccgc cgggcgcggt tgcggtatgt gccgggtcac tgttgcccag
     3241 cggctggtaa tgggtaaagg tttctttgct cgtcataaac atcccttaca ctggtgtgtt
     3301 cagcaaatcg ttaacggcat cagatgccgg gttaccagca gccagcggtg ccggtgcccc
     3361 ctgcatcaga cgatccagcg cagtgtcact gcgcgcctgt gcactctgtg gtgcaactgc
     3421 cagaatgcgg cgggccgttt ccacggtcat accgggggtt tcggccagca cgcgtgcctg
     3481 ttcttcgcgt ccgtgagcct cctcacagtt gaggatcccc ataatgcgac tgttttctgc
     3541 cgcaaccgct gcggtgatct gcgcgttcac gtccggctgc gcagcgctgg cgttctcgcc
     3601 ctccgtcgct
//
LOCUS       DAJTZL010000276         3608 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.279, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000276 DAJTZL010000000
VERSION     DAJTZL010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3608
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.279"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..1627)
                     /gene="tssM"
                     /locus_tag="OO544_004044"
     CDS             complement(<1..1627)
                     /gene="tssM"
                     /locus_tag="OO544_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001612399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HCR6769510.1"
                     /translation="MFRLPTPRLLSGLKSALRPAMPRFKVSAFWLLILAWIFLLVWIW
                     WKGPMWTLYEEQWLKPLANRWLATAAWGIIALVWLTVRVMKRLQQLEKMQKQQREEAV
                     DPLSVELNAQQRYLDRWLLRLQRHLDNRRFLWQLPWYMVIGPAGSGKTTLLREGFPSD
                     IIYAPEGARGAEQRLYLTPHVGKQAVIFDIDGTLCAPADADILHRRLWEHALGWLKEK
                     RARQPLNGIILTLDLPDLLTADKRRREHLLQTLRSRLQDIRQHLHCQLPVYVVLTRLD
                     LLQGFAALFQSLNRQDRDAILGVTFTRRAHENDDWRTELNAFWQTWVDRMNLALPDLM
                     VAQTHTRASLFSFSRQMQGSREPLVSLLEGLLDGENMNVMLRGVYLTSSLQRGQMDDI
                     FTQSAARQYRLGNNPLASWPLVDTAPYFTRSLFPQALLAEPNLATESRAWLIRSRRRL
                     TVFSATGGVAALLLITGWHHYYNGNYQSGITVLKQAKAFMDVPPPQGEDDFGNLQLPL
                     LNPVRDATLAYGDWGDRSRLADMGLYQGRRIGPY"
     gene            complement(1646..3058)
                     /gene="tssA"
                     /locus_tag="OO544_004045"
     CDS             complement(1646..3058)
                     /gene="tssA"
                     /locus_tag="OO544_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HCR6769511.1"
                     /translation="MAMDLRDPNVWISHLLENLPEEKLASALKDDNPNWEYIDGEIVK
                     LGSLAHSQLDIPELQRRGLVILASESKDFRLLAHLLRTLQHAGDPHLALRLLALYVEH
                     YWAVAAPQNMAHKKRFASQVIKRFETGIEVFSQNAATAQRDALSGELAKLAQCWQSHN
                     APELAQATDDLFALYQRAFNRAAPAPVPTPAASGSSPQTTATSESGVTQPSAPAPQIV
                     IDSHDDKAWRDTLLKVAAILCERQPDSPQGYRLRRHALWQNITSTPQAESDSRTPLAA
                     VSADMVADYHAQLGSADMALWQQVEKSVLLAPYWLDGHCLSAQTALRLGYKQVADAIH
                     DEVIRFLERLPQLTGLLFNDHTPFISEQTKQWLAASPDAKVAPVAQIGEESKAARACF
                     AEQGLEAALRYLDMLPEGDPRDQFHRQYLAAQLTEEAGLVQLAQQQYRMLFRMGLQMM
                     VADWEPSLLEQLEQKFTAEQ"
     gene            complement(3063..>3608)
                     /gene="tagO"
                     /locus_tag="OO544_004046"
     CDS             complement(3063..>3608)
                     /gene="tagO"
                     /locus_tag="OO544_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagO"
                     /protein_id="HCR6769512.1"
                     /translation="RLDCYDRLLAPLSPSGFDGALVKAGFVGEAWTRATEQEKRREGN
                     TTELLVTQVPGERPTVVITTPAIGHVPPRPVLMFSCVDNITRMQVALMHPLDVHDIAV
                     TLNADNRALRSHWFVRENGTLLESSRGLSGIDEIKQLFGAKTLTVDTGADNAAGKLTF
                     NIDGLARAIAPLRDACHWAGE"
BASE COUNT          741 a         1062 c         1047 g          758 t
ORIGIN      
        1 cataaggccc gatacgtcgt ccctggtaca gtcccatatc ggccagacgg ctgcggtcgc
       61 cccagtcgcc ataggccagt gtggcatcgc gtaccgggtt caggagcggc agttgcaggt
      121 tgccaaagtc atcttccccc tgcggaggcg gcacgtccat aaaggctttg gcctgcttaa
      181 gcacggtgat gccggactga tagttaccgt tgtaatagtg atgccagccg gtgatgagca
      241 gcagtgctgc cacgccgcct gtggcagaga aaaccgtcag gcggcgacgg gaacgtatca
      301 gccaggcgcg gctctctgtt gccagattag gctctgcgag taatgcctgc gggaacaggc
      361 tgcgggtgaa ataaggcgcg gtgtccacca ggggccagga cgccagtggg ttattgccca
      421 gccggtactg gcgggcggca gactgggtga atatatcatc catctgtcca cgctgaagcg
      481 aagaggtgag atagacacca cgcagcatca cgttcatatt ttcgccatca agcagaccct
      541 caagcagtga caccagcggt tcacggcttc cctgcatctg gcgggaaaaa ctgaataaag
      601 acgcgcgggt gtgagtctga gcgaccatca gatccggcaa cgccagattc attcgatcca
      661 cccatgtctg ccagaaagca ttcaactctg ttcgccagtc atcattttca tgggcacggc
      721 gggtgaacgt gacgcccaga atcgcatcgc gatcctgtct gttgagtgac tggaacaggg
      781 cggcaaaacc ctgtaataaa tcaagccggg tcagtaccac gtaaaccggt aactggcagt
      841 gaagatgctg gcgtatatcc tgtaagcggc tgcgcagcgt ctgtaacaga tgctcgcggc
      901 ggcgtttgtc tgcggtcagc agatcgggta aatcgagtgt cagaataatc ccgttcagcg
      961 gctggcgcgc gcgcttttct ttcagccagc cgagggcatg ttcccacaga cggcgatgca
     1021 ggatatccgc atcagcggga gcgcagagtg tgccgtcgat atcaaagatc accgcctgtt
     1081 taccgacatg gggcgtgagg tacaggcgtt gttctgcgcc gcgtgcgccc tccggggcat
     1141 aaataatgtc ggacggaaac ccctcgcgca gcagtgtcgt tttgccactg cccgccgggc
     1201 cgatgaccat ataccacggc aactgccaca ggaaacggcg gttgtcgaga tggcgttgca
     1261 ggcgcagcag ccagcggtca agataacgct gctgggcgtt cagttccacg ctgagcggat
     1321 caacggcttc ctcgcgctgt tgcttttgca ttttttcaag ctgttgcagg cgcttcatca
     1381 cccggacggt aagccacacc agggcaataa tcccccacgc cgccgttgcc agccagcggt
     1441 tcgccagtgg tttgagccac tgttcttcat acagcgtcca catcgggcct ttccaccaga
     1501 tccacaccag cagaaaaatc cacgccagta tcagcagcca gaaagcagag actttaaacc
     1561 ggggcatcgc cggtcgcagg gctgatttga gtccgctgag tagtcggggt gttggtaatc
     1621 tgaacacaaa aactcctgtt ctcttttatt gttctgccgt gaatttttgt tctagctgct
     1681 caagcaggga cggctcccag tctgccacca tcatctgcaa tcccatcctg aacaacatcc
     1741 ggtactgctg ctgcgcaagc tgtaccagcc ccgcctcttc cgtcaactgt gcggcaaggt
     1801 actggcggtg aaactgatcg cggggatcgc cttcgggtag catgtccaga tatcgcagcg
     1861 ccgcctccag accctgctca gcaaaacagg ctctggctgc cttcgattcc tcaccgattt
     1921 gcgcaaccgg tgcaactttg gcgtcgggcg aagctgccag ccattgtttc gtctgctcac
     1981 tgataaacgg tgtgtggtca ttaaacagca gcccggtaag ctggggcaga cgctcaagga
     2041 agcggatgac ctcatcgtgg atggcgtctg ccacctgttt gtaacccaga cgcagcgccg
     2101 tctgtgcaga aaggcagtga ccatccagcc agtaaggagc cagcagtacg cttttctcca
     2161 cctgctgcca cagcgccata tcggcactac cgagctgagc atggtaatct gccaccatat
     2221 cggcggagac cgcagccagt ggagtacggc tatcgctttc cgcctggggc gtactggtga
     2281 tgttctgcca aagcgcatgg cggcgcagac ggtagccctg cggtgaatcc ggctggcgtt
     2341 cacataaaat ggccgccact tttaacagcg tgtcgcgcca ggctttgtcg tcgtgactgt
     2401 cgatgacgat ttggggagcc ggagcactgg gttgcgtcac gccgctttca gacgtggcgg
     2461 tggtctgtgg tgaactaccg gaggccgccg gagtggggac aggagccggt gccgcacgat
     2521 taaacgcacg ctggtacagg gcaaaaagat catcagtagc ctgtgccagt tccggggcgt
     2581 tatgtgactg ccagcattgc gccagttttg ccagttcccc tgacagcgca tcgcgctgcg
     2641 ctgtggcggc gttctgtgaa aaaacttcaa tacctgtttc aaagcgttta atgacctggc
     2701 tggcaaagcg ttttttatgc gccatattct gcggcgcagc caccgcccag taatgctcca
     2761 catacagcgc cagcaggcgc aatgcgagat gaggatcacc ggcgtgttgc agggttctta
     2821 gcagatgcgc cagcagacga aaatccttac tctccgaagc cagaatgacc agcccccggc
     2881 gctggagttc cgggatatcc agttgactgt gggccagcga tcccagcttg acgatttcgc
     2941 cgtcgatata ctcccagttc gggttgtcat ctttcagcgc cgatgccagt ttctcttccg
     3001 gcaggttttc cagcaggtgc gatatccaga cattcggatc gcgtaagtcc attgccatat
     3061 ttttattctc ccgcccagtg gcaggcatcg cgcaggggag caatcgccct tgccagcccg
     3121 tcaatgttaa aggtcagctt tccggcggca ttgtctgcgc cggtatccac cgtcagcgtt
     3181 tttgcgccga acagttgctt aatttcatca atcccggata acccccggct ggactccagc
     3241 agggtgccgt tctcgcgcac aaaccagtgg ctgcgcaatg cccggttgtc ggcattcaga
     3301 gtgacggcga tatcatggac atccagaggg tgcatcagcg ccacctgcat ccgggtgata
     3361 ttgtccacgc aactgaacat cagcaccggg cgtggcggaa catgaccgat agccggggtg
     3421 gttatcacca ccgtcgggcg ctcccccggc acctgcgtca ccagcagttc tgtggtgttg
     3481 ccctcgcggc gtttttcctg ctctgtggcg cgggtccagg cctcgcccac aaaaccggct
     3541 ttgaccagcg ccccgtcaaa ccctgacggg gacagcggtg ccagcaggcg gtcatagcag
     3601 tcaagccg
//
LOCUS       DAJTZL010000277         3577 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.280, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000277 DAJTZL010000000
VERSION     DAJTZL010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3577
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.280"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..196
                     /locus_tag="OO544_004047"
     CDS             <1..196
                     /locus_tag="OO544_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6769513.1"
                     /translation="SPKKIGFYTIGDKSIYLYDLRRMDEIMEALDNRSSMDWCVAVHD
                     MNAGFDEKILFPSSVESTAG"
     gene            207..374
                     /locus_tag="OO544_004048"
     CDS             207..374
                     /locus_tag="OO544_004048"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6769514.1"
                     /translation="MTTITKERIELFIKNPLENGLTRGEQMELARIALASLEAEPVVF
                     WFEKYQEGATA"
     gene            482..715
                     /locus_tag="OO544_004049"
     CDS             482..715
                     /locus_tag="OO544_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6769515.1"
                     /translation="MDIAVLEIALVSLAAEPAGKLHEYKPVGYQRLVDELTMLVKQLT
                     WQLRKAKPDCKLPDKAMSYLERNGLISVEDILR"
     gene            1007..1162
                     /gene="hokD"
                     /locus_tag="OO544_004050"
     CDS             1007..1162
                     /gene="hokD"
                     /locus_tag="OO544_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HCR6769516.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            1466..1738
                     /locus_tag="OO544_004051"
     CDS             1466..1738
                     /locus_tag="OO544_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6769517.1"
                     /translation="MPSRLHNSDNSAVFASRHGRRSHAFKSDWYQHPPCTEEQAEWLI
                     QCYRRRGCEVKKALSLDYRHWIISVRLPYSERPTRPSRTFQQRIWR"
     gene            1812..2798
                     /locus_tag="OO544_004052"
     CDS             1812..2798
                     /locus_tag="OO544_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCR6769518.1"
                     /translation="MSAFHNGRILVEPEPKSMRNLPSGVVPAARQPLVEDKTLLPFFS
                     NARVIRAAGGAGALSDWLLHHIKSCQWPHGDYHHSETVIHRYGTGAMVLCWHCDNQLR
                     DQTSESLEQLAHQNLSAWMIDVIGHAISGTQERELSLAELSWWAVRNQVADALPEAVL
                     RRSLGLRAEKIRSVYSESDIIPGEQTATSILKQRTKNIALLPHVHQQQNPPQEKTVVS
                     IAVDPESPAQYLQRQKSQREEMPVYTRWVKTQKCMTCGNQADDPHHIIGHGLGGMGTK
                     ADDLFVIPLCRKCRNELHAGVKDFEEKHGSQLLLLIRFLMHARNSGVLKWKA"
     gene            2799..3179
                     /locus_tag="OO544_004053"
     CDS             2799..3179
                     /locus_tag="OO544_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCR6769519.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRAVALIVR
                     QQRLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLMDDEQFDEINIVR
                     AQPVSGGRLGVKIYPIMLEGQVKK"
     gene            3176..>3577
                     /locus_tag="OO544_004054"
     CDS             3176..>3577
                     /locus_tag="OO544_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCR6769520.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGVSAFLGKMGISMNDRERATELLTEYALSRCDRVAALRKLPAEIKPAVMRIMASY
                     AFEDYARSAASKKQCPCCHGKKFIESEVFTNK"
BASE COUNT          969 a          808 c          979 g          821 t
ORIGIN      
        1 atcaccgaaa aaaattggct tttacaccat tggcgataaa agcatttacc tgtatgacct
       61 acgccgcatg gatgaaatca tggaggctct tgataatcgt tcgtcgatgg attggtgtgt
      121 tgctgttcat gatatgaatg cagggtttga tgaaaagatt ttgttcccct catcagttga
      181 aagcactgcg ggttaaggag taacacatga ccactattac caaagaacgt attgaattgt
      241 tcattaaaaa cccgcttgaa aacgggctta cccgtggtga acaaatggaa ctggcacgga
      301 ttgcgctggc atcgctggaa gcagagccgg ttgtgttctg gtttgaaaaa tatcaagaag
      361 gggctacggc atgacgactt ttaccagaga gcagttaata gctcacgcag aggagactat
      421 tgaagcacag agactgtgca taccgggcac aatcgaccat gacatcatcc gcacatataa
      481 gatggatatt gctgttctgg aaatcgcact ggtatcgctg gcagcagagc cagccggtaa
      541 attgcatgaa tacaaaccag tgggatatca gcgtctggtc gatgagttaa ccatgctggt
      601 aaagcagtta acctggcaac tgaggaaagc gaagccagac tgcaaattac cggataaggc
      661 gatgagttat ctggagcgga acggactgat aagcgtggag gatattttac gatgacctgg
      721 cctgaagcat taacaacggt aggaattgcg atggcggtgg cgctggtggt gtattcgatt
      781 tgccgctggg gataaaaacg gtttgcggga aaaggagagt taagtagaat tgcagcgggt
      841 gcttgaggct atctgtctca ggcatgaaca ccaaaaggca gatagagaaa agccccagtt
      901 aacattacgc gtccggcaag acgcttaaca ttaatctgag gctcaatcta tgaacggcaa
      961 atctaggtta gcctcttacg tgccgaaagg caaggagaag caggctatga agcagcaaaa
     1021 ggcgatgtta atcgccctga tcgtcatctg tttaaccgtc atagtgacgg cactggtaac
     1081 gaggaaagac ctctgcgagg tacgaattcg aaccggccag acggaggtcg ctgtcttcac
     1141 agcttacgaa cctgaggagt aagagaccag gcgggggaga aatccctcgc cacctctgat
     1201 gggtcaggca tcctcaatgc acccacactt aacccgcttc ggcgggtttt tgtttttatt
     1261 ttcaacgcgt ttgaagtttt agatggtgcc ggaatagaat caaaaatact taagtagcgc
     1321 gcagggagaa gagggatgga ccccgaacag gggagtgcta tttatctgga aggattctgt
     1381 tgatgaaaat cgaagaatta cgtgaaattt ttagtgaaga tggcctctat actgtgcgcg
     1441 ttgagaatgg cgctattgtc agccactgcc ttccagacta cacaattctg ataattcagc
     1501 cgtctttgcc agcaggcacg ggcggcgctc tcacgcattt aaatctgatt ggtaccagca
     1561 tcccccatgc actgaagaac aggccgaatg gctcattcag tgttaccgca ggcgcggatg
     1621 cgaggttaaa aaagccctta gcctcgacta ccgtcactgg ataatctccg tcaggctccc
     1681 ttactccgaa cggccaacgc gtccgtcccg cacattccag caacggatct ggaggtaatg
     1741 tgcgggtatt acttcgacct gttctggtac cggaactcgg tctggttatc gttaagccag
     1801 gccgtgaatc aatgtcagca ttccataacg gcagaatact ggtggagccg gaaccaaaaa
     1861 gcatgcgtaa tctgccgtcc ggggtcgttc ctgccgctcg ccagccgctg gtggaagaca
     1921 aaacattgct gccgtttttc agtaacgcac gggtgattcg tgctgctggt ggtgctggtg
     1981 cattgtctga ctggctgttg caccatatta aatcctgcca gtggccacac ggcgattatc
     2041 atcacagcga aaccgtcatt caccgttatg gtaccggcgc aatggtgttg tgctggcact
     2101 gcgacaacca gctgcgtgac cagacatccg aatcactcga gcaacttgct catcaaaacc
     2161 tgtcagcatg gatgattgac gtcatcggtc acgcaataag cggtacgcag gagcgtgaat
     2221 tatctctggc tgaattatcc tggtgggcgg tccgcaatca ggtggcggac gcgctaccgg
     2281 aagcggtatt acgtcgttcg ctggggttgc gtgcggaaaa aattcgctct gtgtacagtg
     2341 aaagcgacat cataccggga gagcagacag ccaccagcat actgaagcag cgcacaaaaa
     2401 atattgcgtt actgcctcac gtccaccagc aacagaaccc accacaggaa aagacggtgg
     2461 tcagcattgc cgttgatccg gagtcaccgg ctcagtatct ccagcgccag aaatcacaac
     2521 gggaagagat gcctgtatac acgcgctggg taaaaacgca gaaatgcatg acgtgtggca
     2581 atcaggcaga tgatccgcat cacatcattg gtcatggact gggagggatg ggaacaaagg
     2641 ctgatgattt gtttgttatt ccgttatgcc gtaaatgccg taacgaacta catgccggag
     2701 taaaagattt tgaagagaaa cacggcagtc agctgttgtt gctgattcgt tttttaatgc
     2761 acgcgagaaa ttcgggtgtt ctgaagtgga aagcataaat gaccgagcgc atagaatttg
     2821 ttttacctta cccgccgacg gtgaacactt actggcgtcg tcgtggcagc acatattttg
     2881 tatcaaaagc cggggagcgt tatcgccggg cagtggcgct tattgttcgc cagcagcggc
     2941 tgaaattaag cctgtccgga aggttggcaa taaaaattat tgcagaacca ccggataagc
     3001 gccgccgtga cctggacaat attctgaaag cgccgctgga tgcgctgacg catgcggggt
     3061 tgctaatgga cgatgagcag tttgatgaaa tcaatatcgt tcgtgctcag ccagtatctg
     3121 gtggacgtct gggggtgaag atttatccca taatgcttga agggcaggtc aaaaaatgaa
     3181 actggaagat ttaccgaaat actactcccc aaaatccccc ggcctgactg atgcatcggc
     3241 ctcaacgtcg aaagatgcgc tgagtatcac tgatgtgatg gccgcgcagg gcatgacaca
     3301 gaatcgggct gagatggggg tttctgcgtt ccttgggaaa atgggcatta gtatgaatga
     3361 cagagagcgg gcaacagaat tgctgacaga atatgcactc agtcggtgtg atcgcgtggc
     3421 ggcgttaaga aaactcccgg cagaaataaa accggcagtg atgcgtatta tggcttcgta
     3481 tgcgtttgaa gattatgccc gtagcgcggc gagcaaaaaa cagtgcccct gctgtcacgg
     3541 aaaaaaattt attgaaagcg aggtttttac aaacaag
//
LOCUS       DAJTZL010000278         3559 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.281, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000278 DAJTZL010000000
VERSION     DAJTZL010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3559
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.281"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            129..1376
                     /locus_tag="OO544_004055"
     CDS             129..1376
                     /locus_tag="OO544_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide MFS transporter"
                     /protein_id="HCR6769521.1"
                     /translation="MALNIPFRNAYYRFASSYSFLFFISWSLWWSLYAIWLKGHLGLT
                     GTELGTLYSVNQFTSILFMMFYGIVQDKLGLKKPLIWCMSFILVLTGPFMIYVYEPLL
                     QSNFSVGLILGALFFGLGYLAGCGLLDSFTEKMARNFHFEYGTARAWGSFGYAIGAFF
                     AGIFFSISPHINFWLVSLFGAVFMMINMCFKDKDHQCVAADAGGVKKEDFIAVFKDRN
                     FWVFVIFIVGTWSFYNIFDQQLFPVFYAGLFESHDVGTRLYGYLNSFQVVLEALCMAI
                     IPFFVNRVGPKNALLIGVVIMALRILSCALFVNPWIISLVKLLHAIEVPLCVISVFKY
                     SVANFDKRLSSTIFLIGFQIASSLGIVLLSTPTGILFDHAGYQTVFFAISGIVCLMLL
                     FGIFFLSKKREQIVMETPVPSAI"
     gene            complement(1905..2105)
                     /gene="torI"
                     /locus_tag="OO544_004056"
     CDS             complement(1905..2105)
                     /gene="torI"
                     /locus_tag="OO544_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator inhibitor TorI"
                     /protein_id="HCR6769522.1"
                     /translation="MQHELQPDSLVDLKFIMADTGFGKTFIYDRIKSGDLPKAKVIHG
                     RARWLYRDHCEFKNKLLSRANG"
     gene            complement(2228..2572)
                     /locus_tag="OO544_004057"
     CDS             complement(2228..2572)
                     /locus_tag="OO544_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6769523.1"
                     /translation="MSDLAMKVLKWQSTGDVGISSATLASIACGLKKNIYGHHFGALH
                     DAADFRRCVALVEQIPEIRDSFDKVAKRVPAFKGILNEWDSLVALLKSEMKTYGNKAP
                     ETYRRISELRKD"
     gene            2814..3416
                     /locus_tag="OO544_004058"
     CDS             2814..3416
                     /locus_tag="OO544_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6769524.1"
                     /translation="MSYFLCKKWMINLSGAGKILWALNMKKDSYPYLICMTVSGLIFI
                     FLFFWWRADIYRVTFLNQSISHYYILFSMGIAFLLSLFWVKKGIVKQSGWKSLSAYLK
                     VYAGICIFSGFFLIIPLTTLTYFLPGETSSYVAPYRYTSGSSKSCSGAEVDDPDLHEN
                     IRICYPYGNYEYDNIIYVEKKINTLGAVVTYAQTARDDTE"
BASE COUNT          886 a          714 c          817 g         1142 t
ORIGIN      
        1 gcagtaacgg cgaaaggggc aatgacagcg ctgccatgtc gacaagaact ggaatagtga
       61 gaagtaaacg gcgaagtcgc tcttatctct aaataggacg tgaatttttt aacgacaggt
      121 aggtaattat ggcactgaat attccattca gaaatgcgta ctatcgtttt gcatccagtt
      181 actcatttct cttttttatt tcctggtcgc tgtggtggtc gttatacgct atttggctga
      241 aaggacatct agggttgaca gggacggaat taggtacact ttattcggtc aaccagttta
      301 ccagcattct atttatgatg ttctacggca tcgttcagga taaactcggt ctgaagaaac
      361 cgctcatctg gtgtatgagt ttcatcctgg tcttgaccgg accgtttatg atttacgttt
      421 atgaaccgtt actgcaaagc aatttttctg taggtctaat tctgggagcg ctcttttttg
      481 gcctggggta tctggcggga tgcggtttgc ttgacagctt caccgaaaaa atggcgcgaa
      541 attttcattt cgaatatgga acagcgcgcg cctggggatc ttttggctat gctattggcg
      601 cgttctttgc cggcatattt tttagtatca gtccccatat caacttctgg ttggtctcgc
      661 tatttggcgc tgtatttatg atgatcaaca tgtgttttaa agataaggat caccagtgcg
      721 tagcggcgga tgcgggaggg gtaaaaaaag aggattttat cgcagttttc aaggatcgaa
      781 acttctgggt tttcgtcata tttattgtgg ggacgtggtc tttctataac atttttgatc
      841 aacaactttt tcctgtcttt tatgcaggtt tattcgaatc acacgatgta ggaacgcgcc
      901 tgtatggtta tctcaactca ttccaggtgg tactcgaagc gctatgcatg gcgattattc
      961 ctttctttgt gaatcgggta gggccaaaaa atgcattact tatcggtgtt gtgattatgg
     1021 cgttgcgtat cctttcctgc gcgctgttcg ttaacccctg gattatttca ttagtgaagc
     1081 tgttacatgc cattgaggtt ccactttgtg tcatatccgt cttcaaatac agcgtggcaa
     1141 attttgataa gcgcctgtcg tcgacgatct ttctgattgg ttttcaaatt gccagttcgc
     1201 ttgggattgt gctgctttca acgccgactg ggatactctt tgaccacgca ggctaccaga
     1261 cagttttctt cgcaatttcg ggtattgtct gcctaatgtt gctatttggc attttcttcc
     1321 tgagtaaaaa acgcgagcaa atagttatgg aaacgcctgt accttcagca atatagacgt
     1381 aaacttttcc ggttgttgtc gatatctccc tatccctcaa ccggaaaata ataatactaa
     1441 agtgcttagc ccggctaata atcacctaat ccaaacgcct cattcatgtt ctggtacagt
     1501 cgctcaaatg tacttcagat gcgcggttcg ctgatttcca ggacattgtc gtcattcagc
     1561 gacctgtccc gtgtatcacg gtcctgcgaa ttcatcaagg aatgcattgc ggagtgaagt
     1621 atcgagtcac gccatatttc gctatcagga ttctgtgtga tggttacatc gcccggccca
     1681 gggctgttta gtcatcagcg ctttctgaca gtgctgagat ttcaacctgt tgcagtaaaa
     1741 atgagtagat ataaggcaag tgtgctgcca aacccatctt ttacggggtg aaggtagatt
     1801 tcgtttgaag ggtatctggt gtcccctgca gacatctact tgaggcggca ggggattgat
     1861 tggaatgctg ttttttagat ttgaaaaata ttttacccgc tattttaccc attggcgcgg
     1921 cttaagagct tatttttgaa ttcacaatgg tcacgatata accatcttgc tcgtccgtgg
     1981 ataactttgg ctttaggcag gtcgccggac ttaatccggt catagatgaa ggtcttaccg
     2041 aagccagtat cagccatgat gaatttcaaa tcaaccagtg aatcaggttg tagttcgtgt
     2101 tgcatgagtg ctatctccga atatggaatc gaacctgcaa atcaggcaat aaaaagccgc
     2161 attgatgcag caatggtagg tctggatatc ttgagaaatg aacaggcctc atcgagtgtg
     2221 aggcgggtta gtccttgcgt agctcgctga ttcttctgta agtctctggt gctttgttcc
     2281 cgtacgtctt catttcagac ttcaacagag caacgagtga atcccattcg ttgaggattc
     2341 ctttgaatgc cggaacgcgc tttgcaacct tgtcgaatga atctctgatt tctggaatct
     2401 gctcaacaag tgcaacgcat cgccgaaagt ctgctgcgtc atggagagcg ccgaagtgat
     2461 gaccatagat attctttttc aggccacatg cgattgaggc aagagttgcg ctactgatgc
     2521 cgacatcgcc agttgattgc catttcaaaa ccttcatagc caaatctgac atttcttgtc
     2581 tccataaaac aaaactcgcc gtagcgagct cagataaaag aaatccccgt cagtgcgagg
     2641 atgctgttca ttgctgctat acactttttt gctctcaacg taagcggtag cccattctgt
     2701 tgggttggtc agttgctttt aggaaatgct ctttacccct taaacgtcgg ctgaaagagc
     2761 taaaatccat gcaaaaaatt tacgcaattt tgtgtattat tgtgcagtaa gtaatgagct
     2821 attttctgtg caaaaaatgg atgataaatt tgtccggggc aggaaaaatt ttatgggcgc
     2881 taaacatgaa aaaagattcg tatccttatt tgatttgcat gacagtttca gggctgatct
     2941 ttattttcct tttcttctgg tggcgggcag atatctacag ggtcacgttt cttaatcaga
     3001 gtatatccca ctattacatt ctgtttagca tgggaatagc ttttctgtta tctctgtttt
     3061 gggttaagaa ggggatagta aaacaaagcg gctggaagag tctgtcagca taccttaagg
     3121 tttatgcagg gatatgcata ttttctggat tttttctgat tatacccctt acaacactaa
     3181 cttatttttt gcctggagag acatcgtctt atgttgcacc gtatcggtat acttccggta
     3241 gttcaaaaag ttgttctgga gctgaggtgg atgaccccga tctacatgag aatattcgca
     3301 tttgctatcc gtatggcaat tatgagtacg ataatattat ctatgttgaa aagaaaatta
     3361 atacattagg tgcggtagtg acatatgcac agaccgcgcg tgatgatact gaatgagata
     3421 gtatatagcg ggcaagtttt agttaattta tcgaggtaat ataatttacc tcgactcgtt
     3481 tattctggta tgaatatttc gctttacgat cgatttttat ctgatgatat catgcggttt
     3541 tcatatactg acttactgt
//
LOCUS       DAJTZL010000279         3545 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.282, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000279 DAJTZL010000000
VERSION     DAJTZL010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3545
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.282"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..142)
                     /locus_tag="OO544_004059"
     CDS             complement(<1..142)
                     /locus_tag="OO544_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6769525.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAV"
     gene            complement(188..529)
                     /locus_tag="OO544_004060"
     CDS             complement(188..529)
                     /locus_tag="OO544_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6769526.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(698..889)
                     /locus_tag="OO544_004061"
     CDS             complement(698..889)
                     /locus_tag="OO544_004061"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6769527.1"
                     /translation="MGPALGAARLAQIAANPEKSLIELLPQLPLEQSHLPDAQRYAAY
                     QPRRETFRRLYQQLLPLMA"
     gene            complement(822..2153)
                     /gene="xylB"
                     /locus_tag="OO544_004062"
     CDS             complement(822..2153)
                     /gene="xylB"
                     /locus_tag="OO544_004062"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HCR6769528.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVDSQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLCNVPALIAAAQQADESVEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQKLDYRTGGGCGASTGRSKAGADCGESREIAH"
     gene            complement(2225..>3545)
                     /gene="xylA"
                     /locus_tag="OO544_004063"
     CDS             complement(2225..>3545)
                     /gene="xylA"
                     /locus_tag="OO544_004063"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HCR6769529.1"
                     /translation="QAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRFA
                     ACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHDV
                     DVSPEGASLKEYINNFAQMVDVLAAKQEESGVKLLWGTANCFTNPRYGAGAATNPDPE
                     VFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVEH
                     KHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAGH
                     SFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTGG
                     LNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAARMIEDGELDKRIAQRYSGWNSE
                     LGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
BASE COUNT          923 a          983 c          784 g          855 t
ORIGIN      
        1 gtacggcaaa ataataccct ttttcattta actgcatctc tgcattccat agccctaaca
       61 gataggtaac gataccacca acgagagcta tccatgacac aatactaaag gccggtgaat
      121 aggttgatat tttgttgtcc atcacagtat tcctttcatt cctgaatgtg taagagcttt
      181 atgttgctca taccgagcga aacaaacgca tttgaccaaa caagcagaca aaaaaggcga
      241 tggggtagat caccatttca taagcattga taggtgcatt taaaacaccg ataaacaaga
      301 gaccgatagt gattaacgca actaactggc agatatgggt aaaaaaatta tccagttgcc
      361 cgagtttttc tgcgcgaaga tatgcatagt taccaaaagt tgctgtcatt aacacgccaa
      421 gaaaatagcc ttttccactt agcaatgggc atgagatcca tagcccaacc agataaacca
      481 gcgccccgac aacaaataat agttttgcca cagtttttga ggttttcata acgatctcca
      541 tatctaccag cgatacatta cgagtaacca acgaaagaca aaactgaaga atgccattaa
      601 caaatgattt tcagaataaa ttcatactaa atattaatta attagtgaga tatatagatg
      661 tgaattatcc cccacccggt caggcagggg ataacgttta cgccattaat ggcagaagtt
      721 gctgatagag gcgacggaac gtttcacgtc gtggctgata agcggcataa cgctgcgcat
      781 ctggtagatg cgactgttct aacggtagct gcggcaacaa ctcaatgagc gatttctctg
      841 gattcgccgc aatctgcgcc agccttgctg cgcccagtgc tggccccaca tccccccccc
      901 gtacggtaat cgagcttctg accgctgata tccgccagca tctgacgcca gtactcacta
      961 cgcgccccgc caccaatcaa cgtaacactt tgcggtttaa tgccgcaggc atgcacgaca
     1021 tccatgccat ctgccagcgc ataacccacg ccttccagca ctgctcgcgc cagttcattg
     1081 gggccatgtt gatgagtcaa accaaagaaa acccccttcg cctggggatt attgtgtggt
     1141 gtacgctcgc cggaaagata aggcagaaac caaactggct cgacactttc atcagcctgt
     1201 tgagctgcag cgattaaagc tgggacattg cacaggccgg ttaatttcgc ggcccaatcc
     1261 agacacgatg ctgcactcag cattacagac attaaatgcc aacgctgcgg tagcgcatgg
     1321 caaaagctgt gtacagcgct ttctggcttg cttaagaacc cttcgctgac agcaaaatag
     1381 acccccgacg tccccagcga taacattgcc tgattagcat caaccattcc cacaccaact
     1441 gcaccagctg cattgtcgcc accgcctgcg acaactggca ccgtcgccat accccacgct
     1501 ttcgcaactt caggtaacaa agcaccagta atttcgctgc cttcgtataa tgcgggcatc
     1561 tggtcacgag ataagtcgca agcctgcagc atgacgtcac tccagtcacg ctttgcgaca
     1621 tccagccaca tggtgccagc tgcgtcagac atatcgctgg caaactcccc cgtcatacgc
     1681 agacgcaagt aatctttcgg taataatact ttgtcgattt gacggaatat ctccggctca
     1741 tgccgctgaa cccatagcaa tttaggcgca gtaaatccgg gcatcatcag gttgccggta
     1801 atcactcgtg attgcggaac tctcgcttcc agcaaagtgc actcttgcgc acagcgcccg
     1861 tcgttccaca aaatggcagg gcgcaatacc cgttgttgag catccagtaa ggttgctcca
     1921 tgcatctggc cggcaatacc caatgcttta acgtcctgca gagaatgctg atcgcccaga
     1981 gctttcattg cgcgatcagt tgcctgccac cactgttccg ggtcttgttc cgaccagagt
     2041 ggatgcgggc gcgaaacggt cagcttttcc gtttgcgaat caaccacctc accctgctcg
     2101 ttgagcaaaa taacttttac gcccgaggtg ccaagatcta tcccgatata catatcgatc
     2161 gttccttaaa aaaatgcccg gtatcgctac cgataaccgg gccaacggac tgcacagtta
     2221 gccgttattt gtcgaacaga taatgattta ccagattttc cagttgctcc tggcgaccac
     2281 tctgatgcac cggagacaaa ttatgttcct gagcatattt ggctaaatct gccagtgaca
     2341 tttggccttt caggatttgc tggcccaatt cgctattcca gccggaataa cgctgcgcga
     2401 tgcgtttatc cagctcgcca tcttcaatca tgcgcgctgc aattttcagc gccagtgcca
     2461 tcgtatccat cgcgccgata tgaccgtaaa acagatcata tttatcagta ctttgacgac
     2521 gtactttggc atcgaagttc agaccaccgg tggtgaaacc gcctgctttg agaatttcat
     2581 acatcaccag cgcattctct tccacactgt tcgggaactg gtcggtgtcc cagcccagtt
     2641 gcgcatcgcc acggttggcg tcgacagaac cgaacaggcc aagcgcaatg gcggtggcta
     2701 tttcatgatg gaaagagtga cctgccagcg tcgcgtggtt agcttcaatg ttcagtttaa
     2761 tctctttttc cagaccaaac tgtttcagga agccatagac cgtcgcggca tcgtaatcat
     2821 attgatgttt agtcggttct tgcggtttcg gttcgataag caacgtgccc tggaagccga
     2881 ttttatgttt atgctcaacc accatctgca taaagcggcc cagttgttca cgctcctgac
     2941 gcaagtcggt atttaacagc gtttcgtaac cttcacgacc accccacagg acatagtttt
     3001 caccgcccaa tttatgggtt gcttccatcg ctgtaacaac ttgcgttgcc gcccagctga
     3061 agacttcagg atctgggttc gtcgccgcac ccgcgccata gcgagggttt gtaaagcagt
     3121 tagcggttcc ccacagcagc ttcacgccgc tctcttcttg cttggctgcc aggacatcaa
     3181 ccatttgcgc aaaattattg atgtactctt ttaacgacgc gccctcaggg gaaacatcca
     3241 catcgtggaa gcaataaaat ggcacatgta acttgtggaa aaactcaaat gcgacatctg
     3301 ctttacgctt cgccaacgcc agtgcctcac caggctgctg ccacggacga ttaaacgccc
     3361 ccacaccaaa catgtccgcc ccgttccagc agaaggtgtg ccagtagcag gcggcaaaac
     3421 gcaagtgctc ttccatacgc ttacccaaca ccagttcgtc gggattgtag tgacggaatg
     3481 ctaacgggtt tgaggatttt gagccttcat aacgaacgcg atcgagctgg tcaaaatagg
     3541 cttgc
//
LOCUS       DAJTZL010000280         3506 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.283, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000280 DAJTZL010000000
VERSION     DAJTZL010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3506
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.283"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..1523)
                     /locus_tag="OO544_004064"
     CDS             complement(<1..1523)
                     /locus_tag="OO544_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCR6769530.1"
                     /translation="MKKAPNLKHQPRDKMTEVIIFAGNDAWAHAKQWQEQDGRLAGDS
                     VPPVWLGEQQLAELDKLQIVPDGRYRVRLYQAGLLRPGLVNTIGQKLAAAGVRDADYY
                     PEGMHSQKRENWREYLERERAEQAEKKKVVELPVKKKERVKDDNASSLALNQMGASQR
                     GEVLLAHYGGELAIHADSDTVHHYNGVVWEPVQDKELQRAMAQIFIDAEISYSQNAIK
                     SAVDTMKLSLPVMGNTARNLIGFSNGVFDTRTGNFREHNKNDWLLIASELPFSPPAEG
                     ETLATHAPNFWKWLRRSVAENDRKADRVLAVLFMVLANRYDWQLFIEVTGPGGSGKSV
                     MAEICTMLAGKANTVSASMKALEDARERALVVGFSLIIMPDMTRYAGDGAGIKAITGG
                     DKVAIDPKHKAPYSTRIPAVVLAVNNNAMSFSDRSGGISRRRVIFNFSEVVPENERDP
                     MLAEKIEGELAVVIRHLLTRFSDQDEAKRLLYEQQKSEEALVIKREGDSLVDFCGYL"
     gene            complement(1520..1819)
                     /locus_tag="OO544_004065"
     CDS             complement(1520..1819)
                     /locus_tag="OO544_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HCR6769531.1"
                     /translation="MRTYLSGLTASGYAHPKIIPGAIYLDKNGNRVTVKELMFDRVYF
                     IRDGYSFHSSLNVEIFISRFRREIPLSRNNHVSCMDVDKKLQELKNMIAAWREQK"
     gene            complement(1826..2146)
                     /locus_tag="OO544_004066"
     CDS             complement(1826..2146)
                     /locus_tag="OO544_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001113153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6769532.1"
                     /translation="MPDMTNYQYLINPHFNCEHDIAKKVYSAADGATDNISMAVASIG
                     SLMWHASENEEYDEKAMRIDMGNIGLLLAMLGQFDISLRCTIENATDALNAIEKANTD
                     SNLR"
     gene            complement(2139..2336)
                     /locus_tag="OO544_004067"
     CDS             complement(2139..2336)
                     /locus_tag="OO544_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HCR6769533.1"
                     /translation="MVAVNHIPHLVHTQTAFVWRFLALSAGESQIIHVTAWTEREARN
                     RCPSGCVAVFAARIRQEVCHA"
     gene            2390..2842
                     /locus_tag="OO544_004068"
     CDS             2390..2842
                     /locus_tag="OO544_004068"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6769534.1"
                     /translation="MRGFVVSIAELHVSALVVFTLHLGEYHFKSFLVFTFVLHCIPLL
                     MGGANMSRSSVNNKSQQLNARFPHEVVSGIEASLQPGETKANFIVTAVRGEIARRQAE
                     GSGENPLVSSLDALANVEQIGIKAAEEIGQLITVAREELQRRKAKESE"
     gene            2900..3076
                     /locus_tag="OO544_004069"
     CDS             2900..3076
                     /locus_tag="OO544_004069"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6769535.1"
                     /translation="MTNEESTKKTSPARKRRRRKKEHESERFAPCSFALEKYLKEYSS
                     KERASQLWQRTETD"
     gene            complement(3188..3397)
                     /locus_tag="OO544_004070"
     CDS             complement(3188..3397)
                     /locus_tag="OO544_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family phage regulatory protein"
                     /protein_id="HCR6769536.1"
                     /translation="MREINEDRVIREDECRKLTGVCRTTRYELEKKGRFPSRLNLGGR
                     SVGWLLSEVMEWVKSRDRINSGKAV"
BASE COUNT          880 a          939 c          744 g          943 t
ORIGIN      
        1 atgagatagc cgcagaagtc caccagcgaa tcgccctcgc gttttatcac cagagcttct
       61 tctgatttct gctgctcata cagcaggcgt ttagcttcgt cctggtcaga aaaacgagta
      121 agcaggtggc gaatcacaac cgccagctct ccttctattt tttctgccag catggggtcg
      181 cgttcgttct ccggtacaac ttccgaaaaa ttgaatatca cccgacgacg tgagatcccc
      241 ccgctgcggt cactgaatga catggcgtta ttgttaaccg ccagcactac tgccggaata
      301 cgcgttgagt agggggcttt gtgtttcggg tcaattgcca ccttgtcacc gcctgtaatg
      361 gccttaatcc ctgccccatc accagcgtag cgggtcatat ccggcatgat aatcagcgaa
      421 aagccaacca ctaacgcgcg ttcccttgca tcttccagcg ccttcatgct tgccgatact
      481 gtgttggcct tacccgccag catggtgcaa atctccgcca tcacgctttt accacttccc
      541 cctggacctg ttacctcaat gaataactgc cagtcgtacc ggttcgccag caccatgaat
      601 aatacagcca gtacgcgatc cgccttgcgg tcattctcag ccaccgaacg gcgtaaccac
      661 ttccagaaat tcggcgcatg tgttgccagc gtttccccct ctgctggtgg gctgaaaggt
      721 aattcactgg caattaacaa ccagtcgttt ttgttatgct cccgaaaatt acctgttctg
      781 gtatcaaata ccccgttact gaatccaatc aggttacggg ctgtattccc cattacaggc
      841 aaacttaact tcatggtatc gaccgccgat ttaatggcgt tctgcgaata gctgatctcc
      901 gcatcaatga aaatctgtgc catagctcgc tgtaattctt tatcctgtac tggctcccat
      961 acaacgccgt tgtaatggtg aacagtgtca gagtcagcat gaatcgccag ttcaccgcca
     1021 taatgtgcca ggagaacttc gccgcgttga cttgctccca tctggttaag cgccagtgat
     1081 gaagcgttat cgtcttttac ccgctctttt ttctttacag gcagttcaac tacctttttc
     1141 ttttccgcct gctctgcccg ttcacgttcc agatattcgc gccagttctc ccgtttctgg
     1201 ctgtgcattc cttcagggta ataatcagca tccctgacac ctgccgctgc cagtttctgc
     1261 ccgatggtat taacaagccc cggacgcaat aaccccgcct ggtagagacg cacccgatag
     1321 cgtccgtccg gtacgatttg cagcttatcc agttcggcaa gttgttgttc tccaagccag
     1381 acaggaggca cgctgtcgcc agccagtcgc ccgtcctgtt cctgccactg cttcgcatgt
     1441 gcccacgcat cattacccgc aaaaatgatg acttccgtca ttttgtcacg cggctggtgt
     1501 tttaaatttg gcgctttttt catttctgct ctctccacgc ggcaatcatg tttttcagtt
     1561 cctgtagttt tttatcaaca tccatacatg acacatggtt atttctggaa agcgggattt
     1621 cccgcctgaa tctgctaata aagatctcca cgttcagcga actatgaaat gaatagccat
     1681 cacgaataaa atacacacgg tcaaacatca gttcttttac cgttactctg ttaccgttct
     1741 tatccagata aatagcgccg gggataattt tggggtgtgc ataaccgctg gcagtcaagc
     1801 cagataaata cgttctcatg attatttatc tcagatttga atcagtattc gctttctcta
     1861 tggcatttaa tgcatctgtg gcattttcaa tggtgcaccg taacgaaata tcaaactgcc
     1921 caagcattgc cagtaacaag ccaatattac ccatgtcaat gcgcatagcc ttttcgtcat
     1981 attcctcatt ttctgacgca tgccacatca ggctaccaat tgacgcaaca gccattgata
     2041 tattgtcagt agccccatcc gcagcggaat aaaccttttt agcaatatca tgctcacagt
     2101 taaaatgcgg attaatcagg tactggtaat tggtcatgtc aggcatggca cacctcctga
     2161 cgaatacggg cggcgaatac agcaacacaa cctgacgggc aacggttacg cgcttcgcgt
     2221 tccgtccagg cggttacgtg gatgatttga gattctccgg cactcagggc cagaaaacgc
     2281 cacacaaagg ccgtttgtgt gtgtacaagg tgtggtatat gatttacggc aaccataacg
     2341 gctcctcgtt tacgttgttg gttagaagcc ctgcgagtgg taacgacact tgcggggctt
     2401 cgtcgtttca attgctgaat tgcatgtatc agcacttgtg gtattcacat tacatttagg
     2461 tgaataccat ttcaagtctt ttttggtatt cacttttgta ttacactgca tcccgttatt
     2521 aatgggaggt gcaaacatgt ccaggagttc tgttaacaat aagtcacagc aactgaatgc
     2581 cagatttcca catgaagtag tgagtggcat tgaggcatcc ctacagccag gggaaactaa
     2641 agcgaatttt atagttacgg ctgtacgcgg tgagatcgcc aggcgccagg cagaaggaag
     2701 tggagaaaat ccccttgtgt cttcactgga tgccctagct aatgtcgaac aaatcggcat
     2761 caaggcagcg gaggaaatcg ggcagcttat caccgttgca cgcgaagaac tccagcgccg
     2821 caaggccaaa gaatcagaat aatcactatc agcgccgtgg tgtgaggaac tccggcgcat
     2881 tgctttacag gcgcacacaa tgaccaacga agaatcaacc aaaaaaacat cacctgcacg
     2941 aaaaagacga cgcagaaaga aagagcatga atcagaacga ttcgcgccgt gttcttttgc
     3001 ccttgagaag taccttaaag aatactcatc aaaggagaga gcttcgcagt tatggcaacg
     3061 cactgaaaca gactgatagc attgcccacc agcctgatag cggctatcat tgacatgctt
     3121 atgtttagtg tttccaaact ggcgaccgcc ccaggtcgcc tttgttttat gtgtcatatg
     3181 ctccccttta cactgcctta cctgaattaa tgcgatcccg gcttttaacc cattccataa
     3241 cctcggacag cagccaccct acagaacgac cgcccagatt aagacgtgac ggaaagcgcc
     3301 cttttttctc cagttcgtaa cgtgtagtgc ggcacactcc agttaactta cgacattcat
     3361 cctcacggat tacgcgatct tcatttattt cacgcataca aaaacctaca taaaaattac
     3421 gtatataaac ttttctctta gctgtagata tatgagatcg gatattactt agattctttt
     3481 tcacctctta aattaaaaac acaacc
//
LOCUS       DAJTZL010000281         3460 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.284, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000281 DAJTZL010000000
VERSION     DAJTZL010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3460
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.284"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..404
                     /locus_tag="OO544_004071"
     CDS             <1..404
                     /locus_tag="OO544_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HCR6769537.1"
                     /translation="DEPVFTPIKGVEPPEWFAEEDLPLATIMWQLTTKELCGQGLLCV
                     TDLAVLERWCVAYEFWRRAVKNIAIQGNTITGAMGGRVKNPELTAKKEQESEMSSTGA
                     MLGLDPSSRQRLIGLAGQKKATNPFLKIIES"
     gene            401..2134
                     /locus_tag="OO544_004072"
     CDS             401..2134
                     /locus_tag="OO544_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCR6769538.1"
                     /translation="MSRKSYPNVNAANQYARDVVRGKIVACQFVIQACQRHLDDLMAE
                     KSKSFRYRFDKDLAERAAKFIQLLPHTKGEWAFKRMPITLEPWQLFVICCAFGWVNKG
                     TRLRRFREVYTEIPRKNGKSAISAGVALYCFACDNEFGAEVYSGATTEKQAWEVFRPA
                     RLMCKRTPMLTEAFGIEVNASNMNRPEDGARFEPLIGNPGDGSSPHCAVVDEYHEHAT
                     DALYTTMLTGMGARRQPLMWAITTAGYNIEGPCYDKRREVIEMLNGSVPNDELFGIIY
                     TVDEGDDWTGPQVLEKANPNIGVSVYREFLLSQQQRAKNNARLANVFKTKHLNIWVSA
                     RSAYFNLVSWQSCEDKSLTLEQFEGQPCILAFDLARKLDMNSMARLYTREIDGKTHYY
                     SVAPRFWVPYDTVYSVEKNEDRRTAERFQKWVEMGVLTVTDGAEVDYRYILEEAKAAN
                     KISPVSESPIDPFGATGLSHDLADEDLNPVTIVQNFTNMSDPMKELEAAIESGRFHHD
                     GNPIMTWCIGNVVGKNMPGNDDVVKPVKEQAENKIDGAVALIMAVGRAMLYEKEDTLS
                     DHIESYGIRSL"
     gene            2146..2328
                     /locus_tag="OO544_004073"
     CDS             2146..2328
                     /locus_tag="OO544_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6769539.1"
                     /translation="MIMLILAPLVGVLGALLLAYGAWLIYPPAGFVVAGALCLFWSWL
                     VARYLDRTRQSVGGGK"
     gene            2328..3460
                     /locus_tag="OO544_004074"
                     /pseudo
     CDS             2328..3460
                     /locus_tag="OO544_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466237.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
BASE COUNT          804 a          858 c         1034 g          764 t
ORIGIN      
        1 aagatgaacc tgtttttacg cccatcaaag gtgttgagcc accagagtgg ttcgctgaag
       61 aagatctccc tctcgccacg atcatgtggc aactgacaac caaagaactc tgcggtcagg
      121 gcctgctgtg cgtgactgac ctggcggtac ttgagcggtg gtgcgtggcc tatgagttct
      181 ggcgacgtgc cgtgaaaaat attgccatac agggcaacac catcaccggt gcaatgggcg
      241 gcagggtcaa aaatccggag ctgaccgcca aaaaagaaca ggagtccgag atgagcagca
      301 cgggggcaat gctcggactc gaccccagca gccgccagcg tctgattggc ctggcggggc
      361 agaagaaagc cactaacccg tttctgaaaa ttatcgaatc atgagccgga aatcttaccc
      421 caacgtaaat gctgcaaatc agtatgcccg ggatgtcgtg cgcggaaaga ttgttgcctg
      481 ccagtttgtg attcaggcct gccagcgcca tcttgatgac ctgatggcgg aaaaaagtaa
      541 gtcgtttcgt taccgcttcg acaaggacct ggctgaacgg gccgcgaaat ttattcagct
      601 gttgccgcac accaaggggg agtgggcatt caaacggatg cccatcacgc tggagccgtg
      661 gcagctattt gtgatctgct gtgcgtttgg ctgggtcaat aaaggcaccc ggttgcgccg
      721 cttccgggag gtgtacaccg aaatcccccg taagaacggc aaatcagcaa tctctgccgg
      781 tgttgccctg tattgttttg cctgtgataa cgagtttggc gcggaagtgt attccggtgc
      841 cacgacagag aaacaggcgt gggaagtctt tcgcccggcg cgactgatgt gtaaacgcac
      901 acccatgctg acggaagcgt tcgggattga ggttaacgcc tcaaacatga accgtccgga
      961 ggatggcgcg cggtttgaac cgctgatcgg caacccaggt gatggttcat caccccactg
     1021 tgccgtggtt gatgaatatc acgagcatgc caccgatgcg ctttatacca caatgcttac
     1081 cgggatgggg gcgcgacgtc agccactgat gtgggccatc accaccgccg ggtacaacat
     1141 tgaggggccg tgctacgaca agcggcggga agtcatcgag atgctcaacg gctcggtgcc
     1201 taacgatgaa ctgttcggga tcatctatac cgttgatgaa ggtgacgact ggaccggccc
     1261 gcaggtgctg gaaaaagcca atccaaatat tggcgtgtcg gtttatcgcg aatttttgtt
     1321 aagtcagcag cagcgtgcga aaaataacgc ccgtctggca aacgtcttta aaacaaaaca
     1381 cctcaatatc tgggtgtcgg cgcgttcggc gtatttcaac ttggtgagct ggcagagctg
     1441 cgaggataaa tcactgaccc ttgagcagtt cgaggggcag ccgtgcattc tggcctttga
     1501 cctggcgcgt aagctggata tgaacagcat ggcgcgactt tatacccgcg agattgacgg
     1561 taaaacgcat tactacagtg tggccccgcg tttctgggta ccgtatgaca cggtgtacag
     1621 cgtcgagaaa aatgaagatc gacggacagc cgaacgcttt cagaaatggg tggaaatggg
     1681 cgttctgacc gttaccgatg gtgcggaggt ggattatcgc tacatcctcg aggaggccaa
     1741 agcggcgaac aaaatcagcc cggtcagtga gtcacccatc gaccccttcg gggcgaccgg
     1801 gttgtcacat gaccttgctg atgaagacct gaatcccgtc actatcgtcc agaacttcac
     1861 caatatgtcc gatccgatga aagagctgga agcagcgatt gaatcgggac gttttcatca
     1921 tgacggcaat cccatcatga cctggtgtat cggcaacgtg gtcggcaaaa acatgccggg
     1981 taacgatgat gtggtgaaac ccgtcaaaga gcaggcggaa aacaaaatcg atggtgcagt
     2041 tgcgctgatt atggcggttg gcagagccat gctgtacgag aaagaagaca cgctgtctga
     2101 ccacattgag tcctacggga tccgctcgct ttaactgagg taattatgat catgctgatt
     2161 ctcgcgcctc tggtgggcgt gctgggtgcg cttttgctgg cgtatggtgc ctggctgatt
     2221 tatcccccgg cgggttttgt tgttgccggg gcgctgtgcc tgttctggtc gtggctggtg
     2281 gcgcgatatc tcgaccgtac acggcagtct gtcggcggag gtaaatagtg ttcttttcgg
     2341 gattatttca acgaaaaagc gatgtgccgg tgaccacgcc agcagaactg ctgatgctat
     2401 cgggctgtca tacgacacct ataccggaaa gcggatcagt agccagcggg ccatgcgcct
     2461 gacggcggtc tattcttgcg tcagggtgct ggcagagtct gttggtatgc tgccctgcag
     2521 tctctacaaa atcagcggta ccctgaaaac acgggcggtg gatgaacgac tgcataagtt
     2581 ggtttcggca aaacctaatg gctacatgac accgcaggaa ttctgggagc tggttattgt
     2641 ctgcctgtgt ctacggggga atttttacgc ctacaaggta aaggcactgg gagaagtggt
     2701 ggagcttctt ccgatagatc cgggctgtgt ggaaccgaag ctgaacagcc agtggcagcc
     2761 ggtttatcag gtgacgtttc cggatggttc cgtggatgtg ttgacccaga atgaaatctg
     2821 gcatgtgcgc accctgacgc tggatggact tgtcgggctg aatcccattg cgtatgcgcg
     2881 tgaggccatt tcactggcag cggcaaccga ggagcacggt gccaggttgt ttagtaatgg
     2941 tgcggtgaca tccggtgtgt tgcgtacgga acaaaagctc acgccggatg cttacgagcg
     3001 tctgaagaaa gattttgagg agtgtcacac ggggcttggc aatgctcacc gcccgatgat
     3061 tctggaaatg gggctggact ggaagtcgat ggcgctgaac gccgaggaca gtcagttcct
     3121 ggaaacccgc aagtttcagc tggaagaaat ctgtcgtctg ttccgggtgc cgttgcacat
     3181 ggtgcagaac accgatcgcg ccaccttcaa caatatcgaa gagctggggc tgggatttat
     3241 caactattca ctggtgccgt atctgacccg catcgaacag cggatcaaca ccggactggt
     3301 acgaaaaagt aagcagggcg tttattacgc caaatttaac gccggggcgt tactgcgcgg
     3361 ggatatgaag tcccgttttg aagcctacgc caccgggatc aactggggaa tttactcttc
     3421 caatgactgc cgcgacctgg aagatatgaa tccgcgtccc
//
LOCUS       DAJTZL010000282         3458 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.285, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000282 DAJTZL010000000
VERSION     DAJTZL010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3458
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.285"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            16..1935
                     /gene="ygfT"
                     /locus_tag="OO544_004075"
     CDS             16..1935
                     /gene="ygfT"
                     /locus_tag="OO544_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HCR6769540.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHV
                     VGKGQAANPVACHHCNNAPCVTACPVNALTFQSDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIEVCPTQALRLMDDKGLQWIKVARQRKTAAGKASS
                     DAQPSRSAALLPVNSRKGADKISASERKTHFGEIYCGLDPLQATYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKD
                     HSGAVSIGNLERYITDTALAMGWRPDVSKVVPRIEKVAVIGAGPAGLGCADILARAGV
                     QVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDITFSDL
                     TSEYDAVFIGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESEEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHAMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFDTKAS"
     gene            1935..2423
                     /locus_tag="OO544_004076"
     CDS             1935..2423
                     /locus_tag="OO544_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HCR6769541.1"
                     /translation="MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADVFLPRLKVQ
                     RLDSISAPVMCHQCENAPCVGACPVGALTMGEQVVQTNSARCIGCQSCVSACPFGMIT
                     IQSLPGDTRQQIVKCDLCEQREEGPACVESCPTQALQLLTERELRRVRQQRIVASGEN
                     PL"
     gene            complement(2492..2842)
                     /gene="ghxQ"
                     /locus_tag="OO544_004077"
                     /gene_synonym="ygfQ"
                     /pseudo
     CDS             complement(2492..2842)
                     /gene="ghxQ"
                     /locus_tag="OO544_004077"
                     /gene_synonym="ygfQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxQ"
     gene            complement(2878..3249)
                     /locus_tag="OO544_004078"
                     /pseudo
     CDS             complement(2878..3249)
                     /locus_tag="OO544_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311783.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
     gene            complement(3251..3458)
                     /locus_tag="OO544_004079"
                     /pseudo
     CDS             complement(3251..3458)
                     /locus_tag="OO544_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311781.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          816 a          854 c          971 g          817 t
ORIGIN      
        1 atgaaaggga tgcaaatgaa taagtttatc gctgctgaag ctgcggaatg tataggctgt
       61 catgcttgtg aaattgcctg tgcggtggca cacaatcaag aaaactggcc gctgagtcac
      121 agtgactttc gaccgcgtat ccacgttgta gggaaaggcc aggctgcgaa tccggtggcc
      181 tgccatcact gcaacaatgc cccttgtgtt acggcttgtc cggttaatgc tctgactttc
      241 cagtccgata gcgtacaact ggacgagcaa aaatgtattg gctgtaaaag atgcgcaatc
      301 gcttgcccct ttggcgtcgt tgagatggtc gatacgattg cgcagaaatg cgacctttgt
      361 aaccagcgca gttccggcac gcaagcctgt attgaagtct gcccaacaca ggcgttacga
      421 ctgatggacg ataaagggtt acagtggata aaggtggctc gccagcgcaa aacggcagca
      481 ggaaaagcgt catcagacgc tcagccatct cgcagtgcag cgttgctccc cgttaactcg
      541 cgtaaaggcg cagataaaat ttcagcgagt gaacggaaaa cccactttgg cgaaatctat
      601 tgcgggctgg atccactgca agcgacttat gagagtgacc gctgtgttta ttgtgccgaa
      661 aaagctaact gcaactggca ttgcccgctg cataacgcta ttccggatta catccgtctg
      721 gtacaggaag gaaagattat tgaagcggca gaactttgcc accagaccag ttccttacca
      781 gaaatctgcg gcagggtatg cccacaggac cgtctttgtg aaggcgcatg tactttgaaa
      841 gatcactctg gcgcagtctc tatcggtaat ctggaacgct acatcaccga taccgcgctg
      901 gcgatgggct ggcgtcccga tgtcagcaaa gttgttcccc gtatcgaaaa agtggcggtg
      961 attggcgctg ggccagcagg gttagggtgt gctgatattc tggcgcgcgc gggagttcag
     1021 gttgatgtct ttgatcgcca tccagaaatt ggcggtatgc tgacttttgg cattcctcct
     1081 ttcaaactcg ataaaacggt attaagccag cggcgagaga tattcaccgc aatgggaatc
     1141 gactttcatc ttaactgtga aattggccgc gatattacct ttagcgattt aacttctgaa
     1201 tatgatgcag ttttcatcgg cgtggggact tacgggatga tgcgagcaga tctgccgcat
     1261 gaagatgcgc ccggtgtcat tcaggctcta ccgttcctga ctgcccatac ccgccagctc
     1321 atgggattgc cggagtctga agagtatccg ctgacggacg tggaaggtaa gcgagtcgtg
     1381 gtattgggcg gtggcgatac gacaatggat tgtttgcgga cttccattcg cctcaatgct
     1441 gccagcgtga cctgcgcgta tcgtcgtgat gaagtcagta tgccgggctc gcgcaaagag
     1501 gtggttaatg cgcgcgagga aggtgtcgag tttcagttca atgttcaacc gcaatatatc
     1561 gcttgtgacg aagatgggcg tttaactgcg gtgggcctga ttcgtaccgc catgggtgag
     1621 ccggggccgg atggtcgccg tcgtcctcgt ccggtagcgg gttcagagtt tgaattgccc
     1681 gccgatgttc tgattatggc ctttggtttc caggcgcatg ccatgccgtg gttgcagggc
     1741 agcggaatta aactcgataa atggggcctg attcaaaccg gtgacgtcgg gtatttacct
     1801 acccagacgc atctgaaaaa agtctttgct ggtggtgatg cagttcatgg cgcggatctg
     1861 gttgtcactg caatggccgc aggaaggcag gcggcgcgcg atatgttaac tctgtttgat
     1921 acgaaggcat cgtgatgaaa tcgttaatta tcgttaatcc ggctgactgt attggctgcc
     1981 gtacttgtga agtagcctgt gtggtcgctc acccttcaga acaggagttg aatgccgatg
     2041 tctttttacc ccggctgaag gtacagcgac tggacagcat cagtgcgccg gtgatgtgcc
     2101 atcagtgtga aaacgcccct tgtgttggcg cttgccccgt gggggcgctg acgatgggcg
     2161 agcaggtggt gcagaccaat tctgcccgtt gtattggctg tcagagttgc gtcagtgcgt
     2221 gcccgtttgg aatgatcacc attcagtcgt tgccggggga tacccgacaa caaattgtga
     2281 aatgcgatct ctgtgaacag cgggaagagg ggccagcctg tgttgaatct tgcccgacac
     2341 aggcgctgca gttgcttact gaaagagaac tcaggcgagt ccgccagcag cgtattgttg
     2401 ccagcggtga gaatcctctg tgaaagaaat ctccgcgctg aaaggcgcgg agaatctatt
     2461 agattgccca accacccgcg taaaatgcga ccagtgcgac aggaacgcta atttgttgcc
     2521 cgagtaccag actgaatgcg gtaaatgccg tcaaggaaat cgcacaacca atcgccattg
     2581 gcaaattggc ctacaacccc atcagcaacg agccgaagcc cgcgaccaga caggtggcaa
     2641 caaacacagc tgcgggagga aaaactgctt tgcccagcat tcccggaacg acgataacgg
     2701 aataaaccat ggccagaaag gtcgttaagc cagccagtac ttcctgacga acggtactgc
     2761 cacgagcggt aattttaaaa taattatcca gcgcgccctg tggctttggt gcgtccggtg
     2821 tttgtaggat gtctccagac ataatgatgt cctcacgagg atgttaaagt tttattatta
     2881 gttgcgttcg tacaccagac gaccatcaac gtaggtgcgg tagatcgaac ggtcatcgcc
     2941 caacgtcatc atcacgaaca atttgtcgac taaagaaaca gagttgtcat agcgcagctg
     3001 ctgtagcgga gtggcggtgg gttccatcac cacgaaatca gcctctttgc caggtaaaaa
     3061 gttgccaatc aaatcgtcaa ggcccagaga tttcgctccg ccgagcgtgg ccaggtaaaa
     3121 cgcttcatat gccgagaggc gatagccttg taattgcaac actttgtagg cttcgttcag
     3181 cgtttgcagc atgttgaaag ttgttccggc accgatatcc gttcccatgc ccactttgac
     3241 tttcttctgc ccagatgccg catgtatcgt gaattgcggt agcaatcgcc ggagctgcgc
     3301 catttacacc gatttccgag atcgatttcg ccccgaacgg gccgacttta tcgtcgctcg
     3361 gcaccagcac agcgcggaaa tcacgcggaa tgtcaccaat tttcggtgcg ccgtaactgc
     3421 gtaaatcacg cgttaacggg tgaccttcgg cgtcataa
//
LOCUS       DAJTZL010000283         3433 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.286, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000283 DAJTZL010000000
VERSION     DAJTZL010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3433
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.286"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            167..664
                     /gene="rayT"
                     /locus_tag="OO544_004080"
     CDS             167..664
                     /gene="rayT"
                     /locus_tag="OO544_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HCR6769542.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSQLLTTQYQMLRNAIIKVKR
                     DRPFEINAWVVLPEHMHCIWTLPEGDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPVKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGDVTDINAG
                     ERIIL"
     gene            complement(787..2499)
                     /locus_tag="OO544_004081"
                     /pseudo
     CDS             complement(787..2499)
                     /locus_tag="OO544_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            2471..3256
                     /gene="lafU"
                     /locus_tag="OO544_004082"
     CDS             2471..3256
                     /gene="lafU"
                     /locus_tag="OO544_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HCR6769543.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSITTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDSKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRTR"
     gene            3327..>3433
                     /locus_tag="OO544_004083"
     CDS             3327..>3433
                     /locus_tag="OO544_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6769544.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSR"
BASE COUNT          800 a          920 c          927 g          786 t
ORIGIN      
        1 aaggtattca aggcagggtt aagacgtaag atgcgaaaag cagccatgga cagaaactat
       61 ctggcgtcag tccttgaggg gagcgggctt tcgtaatctt gccctgttcc atcacctcat
      121 tgccgacctt tccttttctt cttaccgttg agaaaaagga gtcgccatgt ctgaatatcg
      181 tcgttattac atcaaggggg gaacctggtt tttcacggtg aatttacgca atcgtcgaag
      241 ccaactcttg accacccagt accagatgct ccgtaacgcc attattaaag ttaagcgaga
      301 caggcctttt gaaatcaacg cctgggtcgt tttgccagag catatgcatt gcatatggac
      361 attacctgaa ggcgatgatg atttttcctc tcgctggcgg gaaattaaaa agcaatttac
      421 ccatgcttgt ggattgaaaa atatctggca accacgtttt tgggagcacg ccatccgcaa
      481 taccaaagat tataggcatc atgttgatta tatttatata aatccagtaa agcatggttg
      541 ggtaaagcaa gtgagtgatt ggccattctc aacgttccat cgcgatgtcg cgcgagggtt
      601 atatcccatc gattgggcgg gggacgtaac ggatataaat gctggcgagc gtatcatttt
      661 ataattatgc gcagatgcct gatgcgacgc tggcgcgtct tatcatgcct acaaacttgt
      721 gcctgaacgg taggccggat aaggcgttta cgccgcatcc ggcagttgtg caccaatgcc
      781 ggatacttac atcaacgccc ccataatctt caactccagc tcgtccggca cttcgttata
      841 cgacagcaca tgcagccccg gcgcaaacaa ccttgcataa cgcgccagca aagggcgcag
      901 ctgcggtggc accagcagca ccgggtcttt ccccgccgct ttcatctgct ctttcacctg
      961 tggcatcgtg ctctggaact ggttgagcat attcgggtcc actggcacgc tgtcgagcat
     1021 cactttaccg ccctgttgcg cctgattcac taggttagtc agcagatttt ccagctcatt
     1081 attcagggta tacaccgtca gctcctgctt gcgaacgaac ggatgggtaa tgctgcgccg
     1141 cagcgccagg cgcacatcgg ctgccagcag aatatgatct ttggtcaccg cgctactggc
     1201 gaccagcacg gtggcgatgg tgacgatatc gcgcagggaa acgccttcgg tcagcagcgc
     1261 ccggtacact ttcagcaact ggctgtaatt gagcaccgcg cttaaatcct ccgccaggcg
     1321 cggtgccatc gacgacaaac ggttatgcaa ctgcgtaata tcgtcatagt taaataaatc
     1381 aggaatatag ctgcgcacaa tcttatttac atgggtagca atcacgctgg cgctgtcgat
     1441 cacctgatac cccatattca gcgccttcgc tttctgcgca ggctggatcc aggtcaccgg
     1501 catcccgtac gccgggtcgt tccccagcac gccgtctatc tcgccgtagg tttcgctgga
     1561 gggcagcgcc atcagtttat ccgccggaat atccgcttca tcagccttaa tgccgttgat
     1621 gaaaatagcg tactggctgg gcttaaggcg gaagttttcc cgaatgcgga tctccggcag
     1681 cagcacgccg ttgccgtcgg atatcacctg tcgcacgccg cgaatacgct gggtgagcgg
     1741 gttgccctgg gctttgtcca ccagcgccac cagcttgtag ccgaggctta agctgatggg
     1801 ctcgatcagc ggaatggttt cccaactgac ctgttgctcg ctggtttcgg tgatggtgcg
     1861 ggtcagcgtt tcgagacttt tctcttccgc ttccgccgcc tgcgggcgtt tgctcatccg
     1921 ccagccggta aagccaagca gggcgctgaa cagcaaaaac ggcagatgtg gcattcccgg
     1981 cactaccgcc agtacgaaca taattccggt agcggtgtag agcaccgatg ggcttgccag
     2041 caactggtta cgcacgtcat gggcgatatc gccgttgtcg ctgacgcggg tgacgataat
     2101 cgccgccgcg gtggagagta gcagggaagg gatttgcgcc accagcccgt cgccgatggt
     2161 catcagcaca tactgctgga aggcggcgtc ggcgctcagg ttgtatttga agatcccgat
     2221 acagacgccg ccgatcaggt tgatcgccag aatcatcatc ccggcgatag catccccgcg
     2281 cacaaatttc gatgccccgt ccatcgcgcc gtagaaatcg gcctcgctgg caacatcttt
     2341 acgtcgggtt tgtgcctgcg cctgattgat caacccggca ttaagatcgg cgtcaatcgc
     2401 catctgctta ccgggcatcg cgtccagggt aaagcgggca gaaacctcgg agatacgctc
     2461 ggcccctttg gtgacgacaa taaagttgat tgtcaacagc gtcagtaaat ccgagcgtga
     2521 aagcattatc gccgcgctgc acgggcagtc gatttttaac ggtggcgggc tgtcgccgtt
     2581 aaataaaatc agcccgtctc atccgccaaa acctgccacc gtggcggtgc cggaagaaac
     2641 ggagaaaaaa gcgcgcgacg tcaatgagaa aacggcgctg ctgaagaaga aatccgccac
     2701 cgagcttggc gagctggcaa ccagtatcac caccattgcc cgtgatgcgc atatggaagc
     2761 gaatctggag atggagattg ttccccaggg attacgcgtg ctgattaaag acgaccagaa
     2821 tcgcaatatg ttcgaacgcg gcagcgcgca gattatgccg ttctttaaaa cgctgctggt
     2881 ggagctcgcg ccagttttcg actcgctcga taacaaaatt attattaccg ggcataccga
     2941 tgcgatggct tacaaaaaca atatctacaa caactggaac ctttcgggcg atcgcgcgct
     3001 ttcggctcgt cgggtgctgg aagaggccgg aatgccggaa gataaagtga tgcaggtaag
     3061 cgcaatggcg gaccagatgc tgctggattc caaaaatccg caaagcgcgg gcaaccggcg
     3121 cattgagatt atggtgctga ccaaaagtgc gtccgatacg ctgtatcaat actttggtca
     3181 gcatggggat aaagtggtgc agccgctggt gcaaaagctg gataagcagc aggtgctttc
     3241 gcagcgaacg cgttaaatgc tgaatcttta cgcatttctc aaactctgaa atcactgtat
     3301 actttaccag tgttgagagg tgagcaatgc gtaaaatcat tcatgtggat atggactgct
     3361 ttttcgcagc ggtggagatg cgcgacaatc ccgccctgcg cgatatccct attgctattg
     3421 gcggcagccg cga
//
LOCUS       DAJTZL010000284         3416 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.287, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000284 DAJTZL010000000
VERSION     DAJTZL010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3416
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.287"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            54..1274
                     /locus_tag="OO544_004084"
                     /pseudo
     CDS             54..1274
                     /locus_tag="OO544_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
     gene            1247..1876
                     /locus_tag="OO544_004085"
     CDS             1247..1876
                     /locus_tag="OO544_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HCR6769545.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRIKAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLQKSIEIDGFTQPIVVTHTDKNAMEIV
                     DGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIRHNRARGRHQITAMSEIV
                     RELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQYSRAWTVK"
     gene            complement(1877..3037)
                     /gene="ybdL"
                     /locus_tag="OO544_004086"
     CDS             complement(1877..3037)
                     /gene="ybdL"
                     /locus_tag="OO544_004086"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HCR6769546.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNSSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHSQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            3146..>3416
                     /locus_tag="OO544_004087"
     CDS             3146..>3416
                     /locus_tag="OO544_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415132.1"
                     /note="member of the iron-containing alcohol dehydrogenase
                     family; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR6769547.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLNDFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVV"
BASE COUNT          908 a          866 c          827 g          815 t
ORIGIN      
        1 catcaccgtc ttatctcact gttcaccctg taacgttcag agagcgtctt cagatgtcta
       61 tttataaaat tcctcttccg ctcaatattc tggaagctgc acgagagcgt attacctgga
      121 cactcaacac cctaccccgc gtttgtgtct cattttctgg cggcaaaaac tctggtttaa
      181 tgctgcatct gacagccgaa cttgcccgac aaatgggcaa aaaaatctgc gttttgttta
      241 tcgactggga ggcgcaattc tcttgcacca ttaactatgt tcagtccctg cgcgagttgt
      301 acaccgatgt catcgaagag ttttactggg ttgcgctccc gcttacgacg caaaattccc
      361 tttcacaata ccaacccgaa tggcagtgct gggaacctga tgtcgaatgg gtgcgtcagc
      421 ccccgcaaga tgcgataacc gatcctgact ttttctgctt ttaccagcca ggcatgacct
      481 tcgaacaatt tgtacgtgag tttgccgaat ggttttcaca aaaacgtccg gtggcgatga
      541 tgatcggcat ccgtgcggat gagtcctaca accgttttgt cgccatcgcc agtttaaata
      601 aacaacgttt tgccgacgat aaaccatgga ctacggccgc accaggcggt catagctggt
      661 acatttaccc catttacgac tggaaagtgg ctgatatctg gacctggtat gcaaatcatc
      721 aaaatctctg caacccactg tataacctaa tgtatcaggc aggcgttcct ctgcgtcata
      781 tgcgaatttg agaacctttt ggcccggagc aacgacaagg attatggctc tatcacgtta
      841 tcgaaccgga tcgctgggct gctatgtgcg cacgagtcag cggggtaaaa agtggcggca
      901 tttacgccgg acatgataat catttctatg ggcatcggaa aatcctcaag ccagaacatt
      961 tagactggca agaatatgca ttattgctgc tcaatagcat gccggaaaaa acagctgagc
     1021 attaccgcaa taaaatcgcc atttatttgc actggtatca gaaaaaaggc atcgaggttc
     1081 cacaaaccca gcaaggggac attggcgcga aagatatccc ctcctggcgg cggatatgca
     1141 aagttttact caataacgat tactggtgtc gggcattatc atttagtcct acgaaagcga
     1201 agaactatca gcgttataac gaacggataa aaggaaaacg tcaggaatgg gggatactat
     1261 gcaacaacga ttaacgcagg atttaacgca atttctcgcc agcttgccgg aagatgatcg
     1321 catcaaagcg attaatgaaa tccgcatggc gatccatcag gttagcccct ttcgtgaaga
     1381 gccagtggat tgcgttctgt gggttaaaaa cagccaactt atgcctaatg attacaaccc
     1441 aaacaacgtg gcaccacccg aaaaaaaact gctgcaaaaa tccattgaaa tagatggttt
     1501 tacccaaccc attgtggtca cacatacgga taaaaatgcc atggaaatcg tggatggttt
     1561 tcaccgccat gaaattggta aaggctcatc atcattaaaa ttacgcttaa aaggttattt
     1621 gccggtgacc tgtctggaag gtacgcgtaa tcaacgcatc gccgcgacta ttcgtcataa
     1681 tcgcgctcgt ggacgccatc aaatcaccgc catgtcggaa atcgtccgtg aacttagcca
     1741 gttgggatgg gacgataata aaattggcaa agagctggga atggacagcg acgaagtgct
     1801 gcgcctgaag caaattaacg gcctgcaaga gctgtttgcc gaccgtcaat attcccgcgc
     1861 ctggacggtt aaataactaa agctggcgca ggcgttcagc tgctgccagc aacgtcgatt
     1921 ccttcttggc aaaacagaga cgaatcagtt tatgtgggaa gggatcggcg caaaacaccg
     1981 acagcggaat cgccgctacg ccgtgctcct gcgtcagcca ctggcaaaac tcaacatcat
     2041 ccagggtaga aaccgcgctg taatccacca gcaaaaagta tgtaccttca cacggtaaaa
     2101 tctccagccg gctttcattt aaggcattta ccagaatatc gcgcttctgg cgataaaagt
     2161 ccggtaacgc aagataatgc tcaggttctg cacgtagcat atcagcaagc gccagctgtg
     2221 ccggggtatt caccgaaaag gtcagatact gatgtacctt gcgaatttcg gcgctgatgg
     2281 gcgctggcgc aacacaataa cccactttcc agccggtcat atgataggtc ttgccaaatg
     2341 aagaaaccgc cactgcccgc tcacgcagct gcgaatgcgc cagcacactg gcatggccct
     2401 gttgtgaaaa gttgatgtgc tcgtagactt catcgctaat gacaaaaatc tcgtgcccgg
     2461 cgatcgcctg ccacaaagcg gcgaaatcag cctgctgcca gacagttgca ctggagttat
     2521 gcggagtgtt gaggatcacc agtctggtgc gctcgcttaa taatgcggca aattcctgcc
     2581 agtcaacgcg aaaatgcggt ggttgcagtg ccatacgctt cactattccc ccagaaagcg
     2641 cgatggcggg ggcgtaactg tcatagctgg gatcaaaaca aatcacttca tcgccattgc
     2701 gcaccagtgc ggtaatcgcc gcgtataacg cttccgtcgc ccctgccgtt acggtgatat
     2761 cgctatcggc atctggttga tagccataca aacgttccgt tttctgagca atcgcctcgc
     2821 gcaaggcctg cacgccggtc atgggcgcgt attggtttgc cccctgggca acgtggtacg
     2881 ccagccgctc ctgtaaatag cgcggaccat caaaatcagg aaagccttgc gacaggttaa
     2941 tcgcctggtg ttgctgcgcc agcgcgctca tctgggtgaa aatagtggtg ccaagttgtg
     3001 gaagtttgct ttgtggaatc agagggttat ttgtcatttt tattgtacct gacgaagttg
     3061 cgtgttgaag gcactataac acgatgctag tatttggcaa tcaagacgtt tagatgtcta
     3121 aataaaacaa taaggacaac acaacatgcc tcacaatcct atccgcgtgg tcgtcggccc
     3181 ggctaactac ttttcacatc caggaagttt caatcacctg aacgattttt tcactgatga
     3241 acaactttct cgcgcggtgt ggatctacgg caaacgcgcc attgctgcgg cgcaaaccaa
     3301 acttccgcca gcgtttggac ttccaggggc aaagcatatt ttgtttcgtg gtcattgcag
     3361 cgaaagcgat gtacagcaac tggcggctga gtccggtgac gatcgcagcg tggtga
//
LOCUS       DAJTZL010000285         3392 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.288, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000285 DAJTZL010000000
VERSION     DAJTZL010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3392
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.288"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            2..3076
                     /gene="lacZ"
                     /locus_tag="OO544_004088"
     CDS             2..3076
                     /gene="lacZ"
                     /locus_tag="OO544_004088"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HCR6769548.1"
                     /translation="MIMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYT
                     NVTYPITVNPPFVPAENPTGCYSLTFNIDESWLQEGQTRIIFDGVNSAFHLWCNGRWV
                     GYGQDSRLPSEFDLSAFLRAGKNRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHK
                     PSTQISDFHVATHFNDDFSRAVLEAEVQMYGELRDELRVTVSLWQGETQVASGTAPFG
                     GEIIDERGGYADRVTLRLNVENPALWSAEIPNLYRAVVELHTDDGTLIEAEACDVGFR
                     EVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSH
                     YPNHPLWYTLCDRFGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRN
                     HPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVD
                     EDQPFPAVPKWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGG
                     FVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQ
                     QQFFQFRLSGQTIEVTSEYLFRHSDNELLHWSVALDGKPLASGEMPLDVAPQDKQLIE
                     LPELPQPESAGQLWLTVHVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAAPHAIPQ
                     LTTSETDFCIELGNKRWQFNRQSGFLSQMWIGAEKQLLTPLRDQFTRAPLDNDIGVSE
                     ATRIDPNAWVERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKT
                     YRIDGSGQMAITVDVEVASDTPHPARIGLTCQLAQVAERVNWLGLGPQENYPDRLTAA
                     CFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETS
                     HRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGSYHYQLVWCQK"
     gene            3128..>3392
                     /locus_tag="OO544_004089"
     CDS             3128..>3392
                     /locus_tag="OO544_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6769549.1"
                     /translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSD
                     TGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFA"
BASE COUNT          754 a          916 c          940 g          782 t
ORIGIN      
        1 tatgatcatg attacggatt cactggccgt cgttttacaa cgtcgtgact gggaaaaccc
       61 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
      121 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggcg
      181 ctttgcctgg tttccggcac cagaagcggt gccggaaagc tggctggagt gcgatcttcc
      241 tgaggccgat actgtcgtcg tcccctcaaa ctggcagatg cacggttacg atgcgcccat
      301 ctacaccaac gtgacctatc ccattacggt caatccgcct tttgttcccg cggagaatcc
      361 gacgggttgt tactcgctca catttaatat tgatgaaagc tggctacagg aaggccagac
      421 gcgaattatt tttgatggtg ttaactcggc gtttcatctg tggtgcaacg ggcgctgggt
      481 cggttacggc caggacagcc gtttgccgtc tgaatttgac ctgagcgcat ttttacgcgc
      541 cggaaaaaac cgcctcgcgg tgatggtgct gcgctggagt gacggtagtt atctggaaga
      601 tcaggatatg tggcgaatga gcggcatttt ccgtgacgtc tcgttgctgc acaaaccgag
      661 cacacaaatc agcgatttcc atgttgccac tcactttaat gatgatttca gccgcgcggt
      721 actggaggca gaagttcaga tgtacggcga gctgcgcgat gagctgcggg tgacggtttc
      781 tttgtggcag ggtgaaacgc aggtcgccag cggcaccgcg cctttcggcg gtgaaattat
      841 cgatgagcgt ggtggttatg ccgatcgcgt cacgctacgt ctgaacgtcg aaaacccggc
      901 gctgtggagc gccgaaatcc cgaatctcta tcgtgcggtg gttgaactgc acaccgacga
      961 cggcacgctg attgaagcag aagcctgcga tgtcggtttc cgcgaggtgc ggattgaaaa
     1021 tggtctgctg ctgctgaacg gcaagccgtt gctgattcgc ggcgttaacc gtcacgagca
     1081 tcatcctctg catggtcagg tcatggatga gcagacgatg gtgcaggata tcctgctgat
     1141 gaagcagaac aactttaacg ccgtgcgttg ttcgcattat ccgaaccatc cgctgtggta
     1201 cacgctgtgc gaccgcttcg gcttgtacgt ggtggatgaa gccaatattg aaacccacgg
     1261 catggtgcca atgaatcgtc tgaccgatga tccgcgctgg ttacccgcga tgagcgaacg
     1321 cgtaacgcga atggtgcagc gcgatcgtaa tcacccgagt gtgatcatct ggtcgctggg
     1381 gaatgagtca ggccacggcg ctaatcacga cgcactctat cgctggatca aatctgtcga
     1441 tccatcccgc ccggtgcagt atgaaggcgg cggagccgac accacggcca ccgatattat
     1501 ttgcccgatg tacgcgcgcg tggatgaaga ccagcccttc ccggctgtgc cgaaatggtc
     1561 catcaaaaaa tggctttcgc tacctggaga gacgcgcccg ctgatccttt gcgaatacgc
     1621 ccacgcgatg ggtaacagtc ttggcggctt cgctaaatac tggcaggcgt ttcgtcagta
     1681 cccccgttta cagggcggct tcgtctggga ctgggtggat cagtcgctga ttaaatatga
     1741 tgaaaatggc aatccgtggt cggcttacgg cggtgatttt ggcgatacac cgaacgatcg
     1801 ccagttctgc atgaacggtc tggtctttgc cgaccgcacg ccgcatccag cgctgacaga
     1861 agcaaaacac cagcagcagt ttttccagtt ccgtttatcc gggcaaacca tcgaagtgac
     1921 cagcgaatac ctgttccgtc atagcgataa cgagcttctg cactggtcgg tggcgttgga
     1981 tggcaagccg ctggcaagcg gtgaaatgcc tctggatgtt gctccacaag ataaacagtt
     2041 gattgaactg cctgaactac cgcagccgga gagcgccgga caactctggc ttacggtaca
     2101 cgtagtgcaa ccgaacgcga ccgcatggtc agaagccgga cacatcagcg cctggcagca
     2161 gtggcgtctg gcggaaaacc tcagcgtgac actccccgcc gcgccccacg ccatcccgca
     2221 actgaccacc agcgaaacgg atttttgcat cgagctgggt aataaacgtt ggcaatttaa
     2281 ccgccagtca ggctttcttt cacagatgtg gattggcgct gaaaaacaac tgctgacgcc
     2341 gctgcgcgat cagttcaccc gtgcaccgct ggataacgac attggcgtaa gtgaagcgac
     2401 ccgcattgac ccgaacgcct gggtcgaacg ctggaaggcg gcgggccatt accaggccga
     2461 agcagcgttg ttgcagtgca cggcagatac acttgccgac gcggtgctga ttacgaccgc
     2521 tcacgcgtgg cagcatcagg gaaaaacctt atttatcagc cggaaaacct accggattga
     2581 tggtagtggt caaatggcga ttaccgttga tgttgaagta gcgagcgata caccgcatcc
     2641 ggctcggatt ggcctgacct gccagctggc gcaggtagca gaacgggtaa actggctcgg
     2701 attagggccg caagaaaact atcccgaccg ccttactgcc gcctgttttg accgctggga
     2761 tctgccattg tcagacatgt ataccccgta cgtcttcccg agcgaaaacg gtctgcgctg
     2821 cgggacgcgc gaattgaatt atggcccaca ccagtggcgc ggcgacttcc agttcaatat
     2881 cagtcgctac agccaacaac aactgatgga aaccagccat cgccatctgc tgcacgcgga
     2941 agaaggcaca tggctgaata tcgacggttt ccatatgggg attggtggcg acgactcctg
     3001 gagcccgtca gtttcagcgg aattccagct tagcgccggt agctaccatt accagttggt
     3061 ctggtgtcaa aaataataat aaccgggcag gccatgtctg cccgtatttc gcgtaaggaa
     3121 atccattatg tactatttaa aaaacacaaa cttttggatg ttcggtttat tctttttctt
     3181 ttactttttt atcatggggg cctacttccc gtttttcccg atttggctac atgacatcaa
     3241 ccatatcagc aaaagtgata cgggtattat ttttgccgct atttctctgt tctcgctatt
     3301 attccaaccg ctgtttggtc tgctttctga caaactcggg ctgcgcaaat acctgctgtg
     3361 gattattacc ggcatgttag tgatgtttgc gc
//
LOCUS       DAJTZL010000286         3375 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.289, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000286 DAJTZL010000000
VERSION     DAJTZL010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3375
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.289"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            6..326
                     /locus_tag="OO544_004090"
     CDS             6..326
                     /locus_tag="OO544_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001409844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine transporter"
                     /protein_id="HCR6769550.1"
                     /translation="MTMALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGASY
                     RYSGAGVGYQVASVVGGGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAMTALLMKD
                     SQRA"
     gene            428..1882
                     /gene="amn"
                     /locus_tag="OO544_004091"
     CDS             428..1882
                     /gene="amn"
                     /locus_tag="OO544_004091"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HCR6769551.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGNYITSGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWNLITADAQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
     gene            2213..2929
                     /locus_tag="OO544_004092"
     CDS             2213..2929
                     /locus_tag="OO544_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR6769552.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNAGVIVFKGSDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
BASE COUNT          933 a          738 c          737 g          967 t
ORIGIN      
        1 gagtcattac catggcgctt gaagcacaat ctattttctg gatagttttc ttctccataa
       61 tgctggcaaa cattgcgcat gacatggtgg tgtgtgtgca acaaccgatg tttaccgaaa
      121 tgtttggtgc cagttatcgc tatagtggcg ctggagtcgg ttatcaggtt gccagtgtgg
      181 ttggcggtgg atttacacct tttattgccg ctgcactcat cacttacttt gccgggaact
      241 ggcatagcgt cgccatttat ttgctggctg gatgcctgat ttccgcaatg accgctttgt
      301 tgatgaaaga cagtcaacgc gcttgatagc ctggcgaaga tcatccgatc ttcgccttac
      361 acttttgttt cacatttctg tgacatacta tcggatgtgc ggtaattgta tggaacagga
      421 gacacacatg aataataagg gctccggtct gaccccagct caggcactgg ataaactcga
      481 cgcgctgtat gagcaatctg tagtcgcatt acgcaacgcc attggcaact atattacaag
      541 tggcgaatta cctgatgaaa acgcccgcaa acaaggtctt tttgtctatc catcactgac
      601 cgtaacctgg gacggtagca caaccaatcc ccccaaaacg cgcgcatttg gtcgttttac
      661 tcacgcaggc agctacacca ccacgattac tcgccctact ctctttcgtt cgtatcttaa
      721 tgaacaactt acgttgctgt atcaggatta tggtgcgcat atctctgtgc aaccctcgca
      781 gcatgaaatc ccttatcctt atgtcatcga tggctctgaa ttgacacttg atcgctcaat
      841 gagcgctggg ttaactcgct acttcccgac aacagaactg gcgcaaattg gcgatgaaac
      901 tgcagacggc atttatcatc caacggaatt ctccccgcta tcgcattttg atgcgcgccg
      961 cgtcgatttt tccctcgcac ggttgcgcca ttataccggt acgccagttg aacattttca
     1021 gccgttcgtc ttgtttacca actacacacg ttatgtggat gaattcgttc gttggggatg
     1081 cagccagatc ctcgatcctg atagtcctta cattgccctt tcttgtgctg gcgggaactg
     1141 gatcaccgcc gaaaccgaag cgccagaaga agccatttcc gaccttgcat ggaaaaaaca
     1201 ccagatgcca gcatggaatt taattaccgc cgatgctcag ggtattactc tggtgaatat
     1261 tggcgtggga ccatcaaatg ctaaaaccat ctgcgatcat ctggcagtgc tacgcccgga
     1321 tgtctggttg atgattggtc actgtggcgg attacgtgaa agtcaggcca ttggcgatta
     1381 tgtacttgca cacgcttatt tacgcgatga ccacgttctt gatgcggttc tgccgcccga
     1441 tattcctatt ccgagcattg ctgaagtgca acgtgcgctt tatgacgcca ccaagctggt
     1501 gagcggcagg cccggtgagg aagtcaaaca gcggctacgt actggtactg tggtaaccac
     1561 agatgacagg aactgggaat tacgttactc agcttctgca cttcgtttta acttaagccg
     1621 ggccgtagca attgatatgg aaagtgcaac cattgccgcg caaggatatc gtttccgcgt
     1681 gccatacggg acactactgt gtgtttcaga taaaccatta catggtgaga ttaaacttcc
     1741 cggtcaggct aaccgttttt atgaaggcgc tatttccgaa catctgcaaa ttggcattcg
     1801 ggcgatcgat ttgctgcgcg cagaaggcga ccgactgcat tcgcgtaaat tacgaacctt
     1861 taatgagccg ccgttccgat aataagttct gaaccttctt atcagaaata gatgtgaagg
     1921 agtaagtaag acacctggca aatagcctgc aataatcgtg ggctgtttgc ttccttgggc
     1981 ggatacgagt tttattatcg tcttaatgat ttccacatat taaaagcaag tatgctttca
     2041 aaacacaatt ataaaaaatc ccgccaacaa tataagtttt tataaaatta tggctttaga
     2101 atatttttat ttctaataga cgagattttt cctgttatga tataatatgc cgaattaaca
     2161 catgttaacg atttaccagt aatgtaaata aattttcgag gagatcattc cagtgggacg
     2221 taaatgggcc aatattgttg ctaaaaaaac ggctaaagac ggtgcaacgt ctaaaattta
     2281 tgcaaaattc ggtgtagaaa tctatgctgc tgctaaacaa ggtgaacccg atccagaatt
     2341 aaacacatct ttaaaattcg ttattgaacg tgcaaagcag gcacaagttc caaagcacgt
     2401 tattgataaa gcaattgata aagccaaagg cggcggagat gaaacgttcg tgcagggacg
     2461 ttatgaaggc tttggtccta atggctcaat gattatcgcc gagacattga cttcaaatgt
     2521 taaccgtacg attgctaacg ttcgcacaat tttcaataaa aaaggcggca atatcggagc
     2581 ggcaggttct gtcagctata tgtttgacaa tgctggtgtg attgtattta aaggatcaga
     2641 ccctgaccat atttttgaaa ttttacttga agctgaagtt gatgttcgtg atgtgactga
     2701 agaagaaggt aacattgtta tttataccga acctactgac cttcataaag gaatcgcggc
     2761 tctaaaagca gctggaatca ctgagttctc aacaacagaa ttagaaatga ttgctcaatc
     2821 tgaagttgag ctttccccag aagatttaga aatctttgaa gggcttgttg atgcccttga
     2881 agatgacgac gatgtacaaa aagtttatca taacgtcgca aatctctaat tatcttttaa
     2941 agaaatctgt ctttacggca gatttcttta atctcatata attcttataa aaaatataag
     3001 attcaactcg tcatattgat tatacccccc cgttcccaga gaaataatat ttattaaaat
     3061 tccagttctt ctttttctga ttacagaagg caaagtggca attacgcata gtttcccgat
     3121 aaagacgcga tagcgacatc ccgcataagg catttttctc tttatctttg tacggtactt
     3181 catggaacag agtttttgac cttgcgaatc gtgatgtctg ttggggaggg gcaatttgct
     3241 cactgaagcg tgagactcga ttaagcgcac gaaacacaga aatcaaaaaa cccggtcact
     3301 tttttacaag gtaaccgggt aaaaataatt tttatttttt aactgttttg agactcatag
     3361 agatgtctca aaact
//
LOCUS       DAJTZL010000287         3318 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.290, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000287 DAJTZL010000000
VERSION     DAJTZL010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3318
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.290"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..321)
                     /gene="fliZ"
                     /locus_tag="OO544_004093"
     CDS             complement(<1..321)
                     /gene="fliZ"
                     /locus_tag="OO544_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HCR6769553.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDMLLDENGWQVEQKSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTV"
     gene            complement(367..1086)
                     /gene="fliA"
                     /locus_tag="OO544_004094"
     CDS             complement(367..1086)
                     /gene="fliA"
                     /locus_tag="OO544_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HCR6769554.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(1251..2825)
                     /locus_tag="OO544_004095"
     CDS             complement(1251..2825)
                     /locus_tag="OO544_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HCR6769555.1"
                     /translation="MAQVINTNSLSLLTQNNLNKSQSSLSSAIERLSSGLRINSAKDD
                     AAGQAIANRFTANIKGLTQASRNANDGISVAQTTEGALSEINNNLQRIRELSVQATNG
                     TNSDSDLNSIQDEITQRLSEIDRVSNQTQFNGVKVLASDQTMKIQVGANDGETIEIAL
                     DKIDAKTLGLDNFSVALGKVPMSSAVALKSEAAPDLTKVNATDGSVGGAKAFGSNYKN
                     ADVETYFGTGNVQDTKDTTDATGTAGTKVYQVQVEGQTYFVGQDNNTNTNGFTLLKQN
                     STGYEKVQVGGKDVQLANFGGRVTAFVEDNGSATSVDLAAGKMGKALAYNDAPMSVYF
                     GGKNLDVHQVQDTQGNPVPNSFAAKTSDGTYIAVNVDAATGNTSVITDPNGKAVEWAV
                     KNDGSAQAIMREDDKVYTANITNKTATKGAELSASDLKALATTNPLSKLDEALAKVDK
                     LRSSLGAVQNRFDSAITNLGNTVNDLSSARSRIEDADYATEVSNMSRAQILQQAGTSV
                     LAQANQTTQNVLSLLR"
     gene            3080..3318
                     /locus_tag="OO544_004096"
                     /pseudo
     CDS             3080..3318
                     /locus_tag="OO544_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460913.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
BASE COUNT          849 a          790 c          711 g          968 t
ORIGIN      
        1 caccgtacct gggctcattt cagtttgctc aaaaagaaat tgcttaaagc cgataatatc
       61 gaaaaaatca ctctgcgttt tgcagtgtaa atcaccacaa aatcggcaca atgccgccca
      121 tgatttttgt tccacttgcc agccattttc atcaagcagc atgtccaggc gggaaatctc
      181 gattttattc actattttgc cgtcgcgaac gagggtaatg cggtcgagta atttacggca
      241 atgcgcgcaa tgagtctggc tgtgtttaaa gtctttaaga tagcggctta atggccgtct
      301 tttcaggtgc tgcaccatca ttaagaactc ctggtagtca aaattaaagt gcggcattta
      361 ctgacgttat aacttaccca gtttagtgcg taaccgttta atggcctggc tgtgtaactg
      421 actgacccgc gattccccga cctccagcac cgcgccaatc tctttgagat tcagctcttc
      481 ctggtaatag agggttaata ccagtttttc gcgctccggc aacgtttcga tggcttccat
      541 cacccgctgg cgcagattac tgtccagcag ttgttgtagc gggttttctc gctgatgatc
      601 atcagtaacc agttcgatgc tatcgccgtg ctcttcgcgc cactcatcgt aggagaagag
      661 ctggctgtta ttggtgtcga gcaacatttg gcgataatcg gcaatatcga tccctaaacg
      721 ttccgctacc tcagtttccg tggcgttgcg gccaagttcc tgctccagtt gccctattgc
      781 ctgtgccact tcacgcgcgt tgcgtcgcac gctgcgcggc acccagtcac ggctgcgaag
      841 ttcatccagc atagcgccac ggatacgctg cactgcgtaa gttgtaaatg ccgttccttg
      901 tagggcgtca tagcgttcga cggcattaag taacccaatg ccgcccgcct gtagcagatc
      961 gtcaagttcc acgctcgcgg gcagtcgaac ctgcaggcgc aatgcttcgt gacgcaccag
     1021 cgggacataa cgctgccaca gcgagtgttt atccattaca ccttcagcgg tatagagtga
     1081 attcacgata aacagccctg cgttatatga gttatcggca tgattatccg tttctgcagg
     1141 ttttttaatc ggacgattag tgggtgaaat gaggggttat ttaggggtta ccggtaaatt
     1201 gcggacagaa aaaaccccgc cggtggcggg gtttgagcga taagtgtaaa ttaacgcagc
     1261 agagacagta cgttctgcgt ggtctggtta gcctgtgcca gaacagaggt acccgcttgt
     1321 tgcaggatct gcgcacgaga catgttagac acttcggttg cgtagtcagc atcttctata
     1381 cggctacggg cagaagacag gtcgtttacg gtgttgccaa ggttggtgat ggcagagtcg
     1441 aaacggtttt gtactgcacc caaagaactg cgcaacttat caacttttgc caaagcttcg
     1501 tctaatttgg ataatggatt tgtggttgct aaggctttca aatctgaggc actcagttca
     1561 gcacctttgg ttgccgtctt attcgtgata ttggctgtat aaaccttatc atcttcacgc
     1621 ataattgcct gtgcagaacc atcatttttt actgcccatt caactgcctt accattagga
     1681 tcagtaataa cagacgtgtt acctgtagcg gcatctacat ttactgcaat gtaggtgccg
     1741 tctgatgttt tagcagcaaa tgaattaggt acaggattcc cttgggtatc ttgtacttgg
     1801 tggacatcta ggttttttcc cccaaaataa acagacattg gtgcatcatt ataagctaat
     1861 gctttaccca ttttacccgc agctaaatca actgatgtgg cagaaccatt atcttcaaca
     1921 aatgcagtta cacgaccacc aaagtttgct aactgaacat ccttaccacc cacctgaact
     1981 ttttcataac ctgtagagtt ttgtttcaat aatgtaaaac cgttcgtgtt ggtattatta
     2041 tcttgaccaa caaaataagt ctgcccttcc acctgtactt gataaacttt tgttcctgca
     2101 gtaccggtcg catcagttgt atcctttgta tcttgtacat taccggtacc aaaataagtt
     2161 tcaacatcag catttttata attgctaccg aatgctttag cacctcccac actaccatca
     2221 gttgcattta ccttagttaa gtcaggagcg gcttcgctct taagtgcaac cgcagaggac
     2281 attggaactt ttcctagtgc tacgctaaag ttatcaagcc ccaaggtttt agcatcaatt
     2341 ttatcaaggg caatctcaat ggtttcacca tcgttcgcac ctacttgaat tttcatagtc
     2401 tgatcagaag ccagaacttt tacaccatta aattgtgtct ggttagaaac acgatcaatt
     2461 tcactaaggc gttgtgtaat ttcatcctga attgaattca ggtcggagtc agagtttgta
     2521 ccattagtgg cctgtactga caattcacga atacgctgta agttattgtt gatttcagaa
     2581 agcgcacctt cagtggtctg cgcaacagaa ataccatcat tcgcgttacg ggaagcctga
     2641 gtcagacctt taatatttgc tgtaaaacgg ttagcaatcg cctgacctgc tgcgtcatct
     2701 ttagcactgt taatacgcag gccagaagag agacgttcaa tggcggagct cagagaagac
     2761 tgagatttgt tcaggttatt ctgggtcaac agcgacaggc tgtttgtatt aatgacttgt
     2821 gccatgatct tttccttatc aattacaact tgatgttatt gggctgttgc ccacggtttc
     2881 tcaccgtacc ccttgtatcg gcacctgaat ttcgaacttt agaaaatttt agtttctctc
     2941 ctgttttttc ttaacggctg aaatatccgt aatatacaac tctactctgc gcatcatctt
     3001 cgtgaatcaa ctattaaact ttgcgcacaa attgccgata acccgcttat ctactgtttt
     3061 caatcaaaag gaattaggta tggcttcatt tacatctctt ggcgttggtt cgaatctgcc
     3121 gctggataca ttgctaaaca acctgaccat cgctgaaaaa aaacgcttaa acccaattac
     3181 tcagcaacag agtgacaata ccgcacgtct gacagcctat ggcacattaa aaatgtgcat
     3241 tagaaaaatt ccagactgca aatactgcgt taaataaagc cgatctgttc aaaagcacta
     3301 ccgttaccag tagcaatg
//
LOCUS       DAJTZL010000288         3285 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.291, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000288 DAJTZL010000000
VERSION     DAJTZL010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3285
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.291"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..229)
                     /locus_tag="OO544_004097"
     CDS             complement(<1..229)
                     /locus_tag="OO544_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6769556.1"
                     /translation="MQSITPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKK"
     gene            403..1116
                     /locus_tag="OO544_004098"
     CDS             403..1116
                     /locus_tag="OO544_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HCR6769557.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSPLTASLVNELAARMPT
                     EHIYLGQSSGLIGEWISQLPVIGAPFGRYLRSKAAHVHEKPSTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGFLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGARILELDGR
                     QPADEILQASLRAIHAALS"
     gene            1148..1906
                     /locus_tag="OO544_004099"
                     /pseudo
     CDS             1148..1906
                     /locus_tag="OO544_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417458.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1952..>3285
                     /locus_tag="OO544_004100"
     CDS             1952..>3285
                     /locus_tag="OO544_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HCR6769558.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVVSALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVVAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     AGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVVLSLIPTLKAFFQRHSLLYNAA"
BASE COUNT          750 a          789 c          854 g          892 t
ORIGIN      
        1 atttttttgc tgttttcacc tgatttcgtt caattgtttt atgtaaggat tttcccatca
       61 acggtaattt tgtcactgca cgaccgacat ttccggcctg ttttcctaaa tgaactcttt
      121 cggcagcacc atatttttcg acaacggtaa taagatgttc acacaccgtt gacttgcctg
      181 aaccatcgct accaataacg gcaattaatg gaggtgttat tgactgcatg aatatatcct
      241 ttaatttaaa catccattaa aaatatttat ttggttaata tgtttttatg aaagcgtaat
      301 tcaggtcaat gtcacaatta accattgtca caataaggtt gaacggatat tctgagcacg
      361 gaccacgtaa tattcaatac attatttact gccgggtttt tcgtgaatca acgtaatatg
      421 tcaataatta attccacgcc agtgcgtgtt attgccattg taggatgtga tggttcaggt
      481 aaatcgcccc tcacggcaag cctggtaaat gaactggcag caagaatgcc aacagaacac
      541 atttatctcg ggcaatcgtc cgggctaatt ggcgaatgga tttcacagct ccctgttatt
      601 ggcgcacctt ttgggcgtta tctgcgaagt aaagcggcac atgtgcacga aaaaccctca
      661 acaccgcctg gcaatattac tgctctggtt atctatctgc tttcctgctg gcgggcgtac
      721 aagtttcgca aaatgttgtg taaaagccag caaggctttc tgctcatcac cgaccgctac
      781 ccgcaggttg aagtgccggg ttttcgcttt gatggcccgc aattggcaaa aaccacgggc
      841 ggtaacggtt ggataaaaat gttaaggcag cgcgagctga agctgtacca atggatggca
      901 tcttatttgc ccgtattgtt gattcgtctt ggcattgatg aacaaaccgc gtttgcgcgt
      961 aaacctgacc accaactggc agcgttacag gaaaaaatcg ccgttacgcc gcaactgact
     1021 ttcaacggcg ccaggatcct tgaactggat ggtcggcaac cagccgatga aatcctgcaa
     1081 gcgtcactac gtgcaattca cgccgcgctt tcctgatcac caaaatggat aggggatgat
     1141 ttaacgaatg gatgcactac aaacacaaac tgttaatagt acaaccgcgc cgcagcccag
     1201 ctacattccg gggctaattg cggtggtcgg gtgcgatggc accggtaaat ccacgctgac
     1261 caccgatctg gtgaaatcat tgcaacagca ctggcaaacc gagcggcgct atctggggct
     1321 gctctcgggc gaagacggcg acaaaatcaa acgattaccg ttggttggcg tctggctgga
     1381 acggcgactg gcggccaaat cctcgaaaac ccaaagcatg aaaaccaaat ctccggcgct
     1441 atgggcggcg gtgattatgt actgcttatc gctgcgaaga atggcgaatc tacgcaaggt
     1501 ttagcgactg gcgcaaagtg gcgttctggt ggtcagcgat cgcttcccgc aggctgaaat
     1561 ttcgggcttt tattacgacg gaccggggat tggcgtcgaa cgtgcgaccg ggaaaatcag
     1621 catgtttctg gcgcagcgcg aacggcgttt ataccaacaa atggcgcaat atcgcccgga
     1681 attaattatt cgcctgggca ttgatattga gactgccatc tcccgcaagc ctgaccatga
     1741 ctatgccgag ctgcaggaca aaatcggtgt catgtcgaag attggctata acggcacaaa
     1801 aattcttgag attgattcca gagcgcccta cagcgaagtg ctggaacagg cacagaaggc
     1861 ggtttctctg gttgccatcg tttctgaccg ccgaagttta acttaaggct ggcgggatac
     1921 tttttactat caacgataag gtcaggctga attggcgggt tttaacatca aacattggtt
     1981 tgcagatggc gcgtttcgca ccattattcg caatagcgcc tggttaggct ccagtaatgt
     2041 cgtgagcgcc ttgctggggc tgttggcgct ctcgtgtgcc ggtaaaggga tgacgcccgc
     2101 catgtttggc gtactggtga ttgtgcaatc gtacgccaag tcgatcagcg attttattaa
     2161 gtttcagaca tggcagctgg tggttcagta cggaacgcca gccttgacca acaataatcc
     2221 gcagcaattt cgcaatgtcg tctcattttc cttctcgctg gatatcgtca gcggcgcggt
     2281 ggcgattgtc ggcggcattg ccttactgcc attcctttcc cattcattag gtctggatga
     2341 ccaaagcttt tggctggcgg cgctgtattg cacgctcatt ccttcaatgg cttcctccac
     2401 gccgaccggc attctgcgtg cggtagatcg cttcgattta attgctgtac agcaggcgac
     2461 gaaacctttt ctgcgcgcag cggggagcgt cgtagcctgg tattttgact ttggttttgc
     2521 gggttttgtt attgcctggt acgtgtcgaa tctggttggc ggcaccatgt actggtggtt
     2581 tgcagcgcgc gaattacgcc gcagaaatat ccataatgcc ttcaaattga atctgtttga
     2641 gtctgcccga cacatcaaag gcgcgtggag ttttgtctgg tcaaccaaca ttgcccactc
     2701 catctggtcg gcgcgtaact cgtgcagcac agtgctggtg gggatcgtgt taggacccgc
     2761 tgccgccggg ttatttaaaa tcgccatgac attctttgac gccgccggaa cgccagcggg
     2821 tttgctgggt aaaagttttt acccagaggt gatgcgttta gatccgcgca ccaccagacc
     2881 gtggttgtta ggtgtgaagt ccggtttact ggcgggcgga atcggtattc tggtggcgct
     2941 cgcggtgttg atcgccggca agccgctcat ttcgctggtg tttggcgtta agtatctcga
     3001 agcgtatgac ctgattcagg tgatgctggg cgcaattgtg atttcgatgc tgggtttccc
     3061 gcaggagtca ttgctattga tggcgggaaa acagcgcgct tttctcgtgg cgcaaaccat
     3121 cgcctcaatc ggctacatcg tattgctgtt catgttctgt catctgtttg gtgtgctggg
     3181 agcagcgttt gcttacttcg gtgggcaatg tctggatgtg gtgctctcgt taattccaac
     3241 tttaaaggca ttttttcagc gtcattcctt gctttataac gcagc
//
LOCUS       DAJTZL010000289         3272 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.292, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000289 DAJTZL010000000
VERSION     DAJTZL010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3272
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.292"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..424)
                     /gene="phnO"
                     /locus_tag="OO544_004101"
     CDS             complement(<1..424)
                     /gene="phnO"
                     /locus_tag="OO544_004101"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HCR6769559.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFSANLRD
                     PNMRYHLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTELSTNVKRHDAHRFYLREGYEQSHFRFT"
     gene            complement(411..968)
                     /gene="phnN"
                     /locus_tag="OO544_004102"
     CDS             complement(411..968)
                     /gene="phnN"
                     /locus_tag="OO544_004102"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HCR6769560.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAELRLREQTQLLVAHRYITRDASA
                     GSENHIALSEQEFFTRAGQNLLALSWHANGLYYGVGVEIDLWLHAGFDVLVNGSRAHL
                     PQARARYQSALLPVCLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCHTLNN
                     DGSLRQSVDTLLTLIHQKEKHHACL"
     gene            complement(968..2104)
                     /gene="phnM"
                     /locus_tag="OO544_004103"
     CDS             complement(968..2104)
                     /gene="phnM"
                     /locus_tag="OO544_004103"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HCR6769561.1"
                     /translation="MIINNVKLVLENEVVHGSLEVQDGEIRAFAESQSRLPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLVNLEKMINAIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAL
                     CRARKIALASHDDATHAHVAESHQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDESNRFTLPQAVKLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRKGNHIHIDHVWRQGKRVF"
     gene            complement(2101..2781)
                     /gene="phnL"
                     /locus_tag="OO544_004104"
     CDS             complement(2101..2781)
                     /gene="phnL"
                     /locus_tag="OO544_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HCR6769562.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLNRASLIVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIHEAKARGAAIVGIFHDEAVRNDVADR
                     LHPMEASS"
     gene            complement(2892..>3272)
                     /locus_tag="OO544_004105"
     CDS             complement(2892..>3272)
                     /locus_tag="OO544_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR6769563.1"
                     /translation="KWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNLVTHPKLVFMD
                     EPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLVMKQGQVVESG
                     LTDRVLDDPHHPYTQLLVSSVLQN"
BASE COUNT          601 a          986 c         1006 g          679 t
ORIGIN      
        1 tggtgaagcg gaagtgactc tgctcgtagc cttctcgcag atagaaacgg tgcgcgtcgt
       61 ggcgcttcac gttggtcgaa agctcggtca tttcggcccc ggcctggcgg gcttcttctt
      121 ctgcccacgc cagcaacttg ctaccgacgt tcaggccgcg cgcctgcggc atcactacca
      181 gttcctgaat ttcgccgatc cagttgacat gatgcagatg aaactgcaaa tgcaggccga
      241 tcatgccgac aacctcgcca tccagcagcg ccagatgata gcgcatgttc gggtcgcgca
      301 gattggcgct aaagcccacg cgaaatgcgt ggtggtcaaa ctccgcctgt tttagctcac
      361 aaatcagcgc gtaaaccgcg tcggtatcat actgcgtggc cgggcgaagc tcacaagcag
      421 gcatggtgtt tctccttctg atgaatcagc gtcagcagcg tgtcgaccga ctggcgcagg
      481 ctgccgtcat tgttgagcgt atggcaatcc tgtggagtat agcgggcggc gcgcgccagg
      541 cgggcgttaa tttcactggc gttttcacgg ccacggtttt ccaggcgctg gcggaggatc
      601 tccggcgaaa cctgtaaaca gacgggcagc agcgccgatt gatagcgcgc ccgcgcctgc
      661 ggcagatggg cgcgtgagcc gttgaccagc acgtcgaatc cggcgtgcag ccagaggtcg
      721 atctcgacgc cgacgccata atacagaccg ttagcgtgcc agcttaaggc cagtaggttc
      781 tgccccgcgc gggtaaaaaa ctcctgctcg ctcagagcga tatggttttc gcttccggcg
      841 ctggcgtcgc gcgtgatgta gcgatgcgcc accagtagct gagtttgttc ccgcaggcgg
      901 agttccgcca gcaggctatc tttcccggag ccggacggcc ccattaacca aatcagtttt
      961 cccatcatca gaacaccctt ttaccctgac gccagacgtg gtcgatatga atgtgattgc
     1021 ccttgcgatg cgccagcacc aagtccgcgc gtttgccctc gccaatcacc ccgcgatcct
     1081 ggagattaag cgcctgcgct ggatttttag tcaccagctt caccgcctgc ggcagcgtaa
     1141 agcggttgct ctcgtcatcg gcgacgcgaa atgccgcatc gagcaggctg gcggggtagt
     1201 agtcggaaga gaggatatcc agcaggccaa gctgcgccag ttcactggcc gccacgttgc
     1261 cggagtgcga gccgccgcgc acaatattcg gcgcgcccat cagcacgttc atgccatgct
     1321 tgcgcgaggc ttccgccgct tcgaacgtgg tgggaaattc ggcgatcacg ctgccaagct
     1381 ggtgagattc agcaacgtgg gcgtgggtgg cgtcatcgtg gctggcaagc gcaatttttc
     1441 gcgcgcggca cagggcggcg atggattcgc gattcggctg cgaccagcgt gcggcgagcg
     1501 ccagttgctc ttcttcgtac tgctgcatct gcgcatcagt gagggagtat ttgccctgat
     1561 aatattcgcg atacttctcg cgattggcga actggcgctg gcccggcgag tggtccatca
     1621 gcgacaccag cgtcaccggc tcgcgctgca ccagtttttc aaacagcggc agcgtggtgt
     1681 gatgcggcag ttcgcagcgc agatgcagac ggtgctcggc gcggttgacg ccgcgtttct
     1741 gcgtctcttc gatggcgttg atcatcttct ccagattcac cagccgatcg ccgccgtcgc
     1801 gcacgtcgcc aattgccacg gcatccagta cggtggtgat gccgctcgcc accatcagcg
     1861 cgtcgtggct gctcatcgcc gagtgggcag gccagtcaac tttcgggcgc ggggtgaaga
     1921 atttatccag attatcggta tgcagctcaa tcagccccgg cagcagccag ccgccttcgc
     1981 cgtccatcgc ctccggcagg cggctctggc tttcggcaaa ggcgcggatt tcgccgtcct
     2041 gcacctccag tgaaccgtgt accacttcgt tttccagcac caatttgacg ttattgataa
     2101 tcatgaagag gcttccattg ggtgcaggcg gtcggcaacg tcattacgta cggcttcgtc
     2161 gtggaagatg ccgacgatgg ccgcgccacg ggctttggct tcgtgaatca gctcgactac
     2221 ggcggcgctg tttttggcgt ccagcgaggc ggtaggttcg tcgagcagca gaatggggta
     2281 gtcgacgata aagccgcggg cgatgttgac gcgctgctgt tcgccgccgg agaaggtcga
     2341 tggcgccaga tgccacaggc gttccggcac gttcaggcgg gtgagcagac gcgcggcttt
     2401 agcggcgcag gcttcacgcg gtacgccggt atcgagtagc ggctgcatca ccacttccag
     2461 cgccgaaata cgcgggatga cgcgcagaaa ctggctcacc cagccaacgg tggttttgcg
     2521 gatctccacc actttgcgcg ctggcgcggt gaccagatcg acccattcgt caccgtgttt
     2581 gatctggatt tgcccttcgt cgggcagata gttggcgtac agcgagcgta gcagggttga
     2641 ttttccgctg ccggaatggc cgtggagcac cacgcattcg cccgcgttga cgatgagcga
     2701 ggcgcggttg aggacgggca ggcgcacgcc gttttgctgg tgcaggatga aggttttact
     2761 gacgttttgt acgttaatca tttttcgcct cgtggttcgg gtgtcggatg cgacgctgac
     2821 gcgtcttatc cgacctacgg ggagcgcatt tgtaggccgg ataaggcgtt tacgccgcat
     2881 ccggcaccgg ctcaattctg caaaaccgat gacaccagca gctgtgtata cggatgatgc
     2941 gggtcgtcga gcacgcggtc ggttaaccca ctctccacca cttgcccctg cttcatcacc
     3001 agcaaacggt ccgccagcag gcgggcgacg cctaaatcgt gggtgacaat cactaccgcg
     3061 aggttcagct ccaccaccag tccgcgcagc aggtcgagca ggcgggcctg caccgacaca
     3121 tccagcccgc cggtcggttc atccataaac accagcttcg gatgcgtcac caggttgcgg
     3181 gcaatctgca aacgctgctg cataccgccg gaaaaggtgg tcggcaggtc gtcgatacgg
     3241 ttggcgggaa tctccacctc ttccagccac tt
//
LOCUS       DAJTZL010000290         3220 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.293, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000290 DAJTZL010000000
VERSION     DAJTZL010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3220
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.293"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            8..511
                     /locus_tag="OO544_004106"
     CDS             8..511
                     /locus_tag="OO544_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR6769564.1"
                     /translation="MNNIAPQSPVMRRLTLLDNPAIARVIRQVSAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYEGEVVGGGGIAPLTGSESDICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(557..973)
                     /gene="rraB"
                     /locus_tag="OO544_004107"
     CDS             complement(557..973)
                     /gene="rraB"
                     /locus_tag="OO544_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HCR6769565.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            1135..2139
                     /gene="argF"
                     /locus_tag="OO544_004108"
     CDS             1135..2139
                     /gene="argF"
                     /locus_tag="OO544_004108"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HCR6769566.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKTFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     "
     gene            complement(2211..>3220)
                     /locus_tag="OO544_004109"
     CDS             complement(2211..>3220)
                     /locus_tag="OO544_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR6769567.1"
                     /translation="LLEKPLNNALLAIRKEHLLLMPYVCNESISYLLGEKGILKEIDD
                     LNAVNNYLLNNKKATDNEINDIKVNLSHILIDSLDDAKVNLTPVIDSILETFLKSPYI
                     NDVRILDWCFNKRMQYFGDSEKIKYACSVINHIDFSCDQSKDFSCDQSKIKIAETLFF
                     NLDKEPYKNSRKLQELIWDKLVAYVNDFSLSNQEKSRLILRLFDDVKLLFNEVPVSIL
                     VNDIFLKDFFMKQPDFAKWYFYQLLKCYEGEQLYLNELGYVYGDEEKTKEIVNKLPGY
                     VVKIFEEKMGNELKIRTRMMKILRNGKINIYGYINEEQLGKLNPPEDLRIAIKKLGWN
                     N"
BASE COUNT          893 a          719 c          675 g          933 t
ORIGIN      
        1 ttaaactatg aataacattg cgccgcaatc acctgtaatg cgtcgcctga cgctgctgga
       61 taatcctgct atcgcccgcg tcattcgtca ggtatccgcc gaatacggtc ttaccgctga
      121 taaaggctac accgtcgccg atccgaatct tgacgagctg tatcaagtgt atagtcagcc
      181 tggccatgca tattgggtgg ttgagtacga gggtgaagtg gtcggcggcg gtgggattgc
      241 gccattaacc gggagtgagt cggacatttg cgaactgcaa aagatgtatt ttcttccggc
      301 tattcgcggt aaagggctag cgaaaaaact ggccttaatg gcgatggagc aggcacgaga
      361 gatgggcttc aaacgctgct atctggaaac gaccgctttt ttaaaggaag ccattgcgct
      421 ttatgagcat ttgggctttg agcatatcga ctatgcgctt ggctgcacgg gccatgtcga
      481 ttgtgaagtg cggatgctac gcgaactcta aaaaaatgcc cgctagtatg tgctatcggg
      541 cattttaatc aattaattag tggcgaaccc cgtcatcgtc ttcatcgaca aaatcttcat
      601 cgtcgccatc ttcgccgtta ggatcttcaa agtaagtgcc ccaaccgtcg tactcaacgt
      661 caaatttctc cgccagcgtc atcagttgtt caacctgggc atcgatcaga tcggcattca
      721 acgcgcactc gctgaggatg tcgcagcaaa tcacgatatc accatcttca acttccagct
      781 cttctggatc ggtcacttcg taaccgagtt taaacgcttc aactgccgct ttttccagag
      841 tttccagatc gtctgcggaa agatggtgtt cgatggtgta caacgcgtcc ggatcgctgc
      901 catcttccag taattcttca ataatcaaac gtgtttcttc acgctgttct tccagttgtt
      961 ccgggtttgc catggctcat tcctcttaat gtcctgccga tactcttatt gtcacacacc
     1021 actgacattg cctccacctt tgtgataaag atttatgctt tagacttgca aatgaataat
     1081 catccatata aattgaattt taattcatta aggcgttagc cacaggaggg acctatgtcc
     1141 gggttttatc ataagcattt cctgaaatta ctcgatttca cgccagctga actcaacagc
     1201 ctgctgcagt tagccgcgaa gctgaaagcc gataagaaaa gcggtaaaga agaagccaaa
     1261 ctcactggta aaaacatcgc gctcatcttc gaaaaagact cgacccgtac ccgatgctct
     1321 ttcgaagttg ccgcatatga ccagggggct cgtgttactt atctcggccc aagcggcagc
     1381 caaattggtc ataaagagtc gattaaagac actgcccgcg tgcttggtcg catgtatgac
     1441 ggtattcagt atcgcggcta tggtcaggag attgtcgaaa cactggcgga atacgctggc
     1501 gtgccggtat ggaacggcct gaccaatgag tttcacccca cgcagctgct ggcggatctc
     1561 ctcaccatgc aggagcattt gcccggcaaa acgttcaacg aaatgacgct agtctatgca
     1621 ggtgacgcac gtaacaacat gggcaattcg atgctcgaag ctgcagcgct taccggtctg
     1681 gatttgcgtc tggtcgcgcc acaggcatgc tggccggaag ctgcgctggt tacggaatgc
     1741 cgcgccctgg cacaacaaaa tggcgggaat attacgctga cggaagatgt agctaaagga
     1801 gttgaaggtg ctgactttat ctataccgat gtatgggtgt cgatgggtga agcaaaagag
     1861 aaatgggctg aacgcattgc attgctgcgt gattatcaag tgaacagcaa gatgatgcag
     1921 ttgaccggta acccggaggt caaattcctc cactgcctgc ccgcgtttca tgacgaccaa
     1981 acgacgcttg gcaaaaaaat ggcggaagag tttggcctac atggcggaat ggaagtgact
     2041 gatgaggtct tcgaatctgc cgccagcatt gtatttgatc aggcggaaaa ccgcatgcat
     2101 accatcaaag cggtgatggt cgcgacgctc agtaaataat tgatatcgct ataaagatgc
     2161 cgggaaatac aaaaacatcc cggcgtcaca ttattttatg cagtgatagt tcaattattc
     2221 caccccaatt tttttattgc tatacgtaaa tcttccggag gatttaattt tcctaactgt
     2281 tcttcattaa tatatccata tatattgatt ttgccattcc ggaggatttt catcattctt
     2341 gttctaattt ttaattcatt acccatcttt tcttcaaaaa tcttaacgac ataacccgga
     2401 agtttattta caatttcttt tgttttttct tcatcaccat aaacatatcc taactcatta
     2461 agatacagct gttcgccctc ataacactta agtaattgat agaagtacca cttagcaaaa
     2521 tcaggttgct tcatgaaaaa atccttcaaa aaaatatcat ttactaaaat gctgacaggc
     2581 acttcattaa atagtaactt aacatcatca aataacctta gtattaagcg tgatttttct
     2641 tgattgctta agctaaaatc attgacatat gcaaccaact tatcccaaat caattcctgt
     2701 aatttacggc tatttttata gggttcttta tcgagattga aaaataaggt ctcagctatt
     2761 ttaattttag attgatcgca gctaaaatct ttagattgat cgcagctaaa atcaatatga
     2821 tttatcacgg agcatgcata ctttatcttt tcagaatcac caaaatattg cattctttta
     2881 ttaaaacacc aatccagtat tcttacatca ttaatatatg gggattttaa aaaagtctcc
     2941 agaatcgaat cgatgaccgg tgtaaggtta acttttgcat catctaagga atcaataaga
     3001 atatgagaca ggttaacttt gatgtcatta atttcattgt cagtagcttt cttattatta
     3061 agaagataat tatttactgc gttaaggtca tctatttctt tcagtatccc tttttcacca
     3121 agcaagtatg aaatcgattc attacataca taaggcatta atagcagatg ttctttcctt
     3181 attgctaata atgcattgtt caatggtttt tctaataaat
//
LOCUS       DAJTZL010000291         3183 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.294, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000291 DAJTZL010000000
VERSION     DAJTZL010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3183
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.294"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(9..635)
                     /gene="dsbA"
                     /locus_tag="OO544_004110"
     CDS             complement(9..635)
                     /gene="dsbA"
                     /locus_tag="OO544_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HCR6769568.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
     gene            complement(652..1638)
                     /gene="srkA"
                     /locus_tag="OO544_004111"
     CDS             complement(652..1638)
                     /gene="srkA"
                     /locus_tag="OO544_004111"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HCR6769569.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEERRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQTEAVGRYLGRMHQTGHKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAEQRMQLETIIEAYEEFGEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(1715..1984)
                     /locus_tag="OO544_004112"
     CDS             complement(1715..1984)
                     /locus_tag="OO544_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HCR6769570.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            2054..2638
                     /gene="mobA"
                     /locus_tag="OO544_004113"
     CDS             2054..2638
                     /gene="mobA"
                     /locus_tag="OO544_004113"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HCR6769571.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLELNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQASGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHVLASRLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            2620..3147
                     /gene="mobB"
                     /locus_tag="OO544_004114"
     CDS             2620..3147
                     /gene="mobB"
                     /locus_tag="OO544_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HCR6769572.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTEKPDEEELDLQFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
BASE COUNT          832 a          771 c          769 g          811 t
ORIGIN      
        1 acattgaatt attttttctc ggacagatat ttcactgtat cagcatactg ctgaacaaaa
       61 acatccatat tgctggtatc cataccctgc ggattcagct gatatttacc gttaacaaac
      121 atcgccggaa cgccacgcaa ttgcacgtca gctgcagctt tttcctgctg agcgaccaga
      181 gatttgacca cgaagctgtt ccacgccgcg tcgtactctt cacctttaat acctgcgttg
      241 ataaatacat cgcggatatc agatgctgaa cgaatggtct gggttttctg tacgccttca
      301 aacagcggaa cagtgacttt gtcttccacg cccagcgcca tcgccacagc ccatgcctga
      361 gtcagatctt tgcccaggtc accacccatg aagttgacgt ggtatttagt catcttcacg
      421 ccttccggca gttttttctt cacattatca gaaatatgca gaacttcttc aaactgatag
      481 cagtgcggac agaagaaaga gaaaaactcc agcacttgcg gcgcgccagc taccggtttt
      541 tccagggtag tgtactgttt accatcttca tactgcgccg ccgatgcgct aaacgctaaa
      601 actaaaccag ccagcgccag ccaaatcttt ttcatgatct actctctccg attaatacat
      661 aggtgttaat tgtaaagggg gttcttgtag aacttttgcc tgttctataa aagtcgccgt
      721 ttgtcgcagc cagtaatctt ccccggttaa ccacgggaaa tttttcggga acgcgggatc
      781 agcccaacgc cgcattagcc aggcaagata ataaaccaaa cgcatggcgc gtaaaggttc
      841 aatcagtccg atttcagcgg tgtcgaactc gccaaattct tcataagctt caataatagt
      901 ttccagttgc atccgctgct cggctttatc gccattgagc aacatccaca aatcctgaat
      961 agctggacca ttacgtgcat catccagatc aacaaacatt ggaccatcgc gccagagaat
     1021 attcccggcg tggcagtctc catgtagccg cagaacggtg aaatcttccc gccagtgtgc
     1081 tgtaacggcg gcgatcagct catctgtcgc tttcaggaat gccgctttca acccggaagg
     1141 tatcagtgta gcgtcctcaa acagcttgcg tggctcaatg agatattcat tcagaccgat
     1201 ggtcgggcga tggataaaaa gctgtttgtg ccccgtctgg tgcatacgcc ctaaataacg
     1261 cccaaccgct tccgtctgat cgatattatc agcttcgaac tggcgaccac cgacgcttgg
     1321 aaaaacagcg aaataaaatc cctgatgatt caataaagtc tgaccgttaa aggccacagg
     1381 tgctgcgacc ggaacttcat catttaccag ctgcaacgca aattgatgtt cttcgaggat
     1441 ttgatcggct gtccaacgtt cagggcgata aaatttgacg acaaaacgtc gacgctcttc
     1501 gtcctgaaat tgatagacac ggttttcata gctgttaagc ggggtaagac cggaatccac
     1561 ccggatccct tgctcaaaca gagcgtccat gatggtatcc gggtgtagtg tctggaaagt
     1621 aaaagcgctg ttgttcatcc catcatccgg aaaacattac gaatgatgaa ggatatcatt
     1681 tcacgatgat tttggtggcg ccgcttacaa gcttttactc tttaattacg ccgcgagcgc
     1741 gtaacagcgc cgttttgaag tcttcttcat aatctttctg caaacccggg atcaccgcat
     1801 ctttcgcgga atcacgcatt ttcaggtgat agatcagaat gtcatccgtc aaatccgcca
     1861 gttcgccgtc aaaacctgac tctttcgcca gtttctgcaa aaattgcaac aggttaaggt
     1921 ctggctcttt ttgccaggct ggctggagga gttcaataac ttcattcaga cgtttacatt
     1981 tcatagtgat gctccttact cttgagacag acacgttagc agggtcaatc ccacaataaa
     2041 agaggcgata tcggtgaatc tgatgacgac gataacaggc gttgtgctgg caggcggtaa
     2101 agccagacga atgggcggcg tagataaagg attgcttgaa ttaaacggca aaccattatg
     2161 gcaacatgtc gctgacgcgc ttatgacgca gctctctcac gtcgtggtta atgccaatcg
     2221 tcatcaggaa atctatcagg caagcggtct gaaagtgatt gaagattcac tggcggatta
     2281 cccaggccct ctggcaggaa tgctttcagt aatgcagcag gaagctggtg agtggttttt
     2341 gttttgtccg tgcgatacgc cttacattcc ccatgtttta gcttcccggc ttaatcatca
     2401 gcgcaaagat gcgcctgtcg tgtgggtcca tgacggtgaa cgcgatcacc cgactattgc
     2461 tctggtaaac cgtgctattg agcctttatt actagaatat ctgcaagcag gagaacgccg
     2521 ggtaatggca tttatgcgtc tggctggcgg ccatgcggtt gatttcagcg atcataaaga
     2581 tgcatttgtt aacgtgaata cgccagagga gcttgcccga tggcaggaaa aacgatgata
     2641 ccgttactcg cctttgccgc gtggagtggt accggaaaaa ctacgctttt gaaaaaactg
     2701 atcccggcat tatgcgccag agggatccgt ccagggctga ttaagcatac gcaccatgat
     2761 atggatgttg ataagccagg caaagatagc tatgagctgc gcaaggctgg cgcggcacaa
     2821 accatcgttg ccagccagca acgatgggcc ttgatgacgg aaaaaccaga cgaagaagag
     2881 ctggatctac agtttctcgc aagtcgaatg gatacctcaa agctggattt gattctggtc
     2941 gaagggttta agcatgaaga gatcgcaaag attgtgctgt ttcgcgatgg agccggacat
     3001 cgacctgaag aattagtgat agacaggcat gttattgctg tagccagtga tgtgccgctt
     3061 aatcttgatg tcgcattact ggatattaat gatgttgagg ggctggctga ttttgtggtg
     3121 gagtggatgc aaaagcagaa cggataagca agagtaggct ggataagacg cgccagtgtc
     3181 gca
//
LOCUS       DAJTZL010000292         3175 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.295, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000292 DAJTZL010000000
VERSION     DAJTZL010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3175
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.295"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            533..2386
                     /locus_tag="OO544_004115"
     CDS             533..2386
                     /locus_tag="OO544_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HCR6769573.1"
                     /translation="MAFKHYDVVRAASPSDLAKRLTQKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVVVSGATEPEWYYVIVLAGQSNAMAYGEGLPLPDSYDAPHPRIKQLARRN
                     TVTPGGEVCVFNDIIPADHCLHDVQDMSTINHPRADLSKGQYGCVGQGLHIAKKLLPY
                     IPNNAGILLVPCCRGGSAFTQGTEGTFSESTGASQDSARWGVGKPLYQDLLFRTKAAL
                     QKNPKNVLLAICWMQGEFDMTNASYAQQPAAFLAMVQQFRADLAGLAAQCHGGSPASV
                     PWICGDTTYAWKQEHGTQYEVVYGAYKGKESQQIYFVPFMTDGSGVNTPTNNPSEDPD
                     IAGSGYYGSASRTNKNWVSSNRPTHFSSWARRGIIPDRMATAILNVAGRTLAFISGKA
                     PEIKPSPGGDTPSGPSEDASIRTISLLPTAGDAAAQGWSIKNGGVQLSDGVFKITKQS
                     NKAWSLTRPVDDAVSLLTRGGRLSCKFRLSGALTNNQFGLGIYLYTDVALPDVVAMTG
                     TGNPFLMSFFTQTTDGKLNLMHHKKAGNTKLGEFGNYSNDWLTLELVFTAGSATVTPK
                     LNGVAGPAFQVIKDSLTLGLNALTLTDITKNAAYGVEIESLVLEINAPASS"
     gene            2537..2752
                     /locus_tag="OO544_004116"
     CDS             2537..2752
                     /locus_tag="OO544_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HCR6769574.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGE"
     gene            2757..3071
                     /locus_tag="OO544_004117"
     CDS             2757..3071
                     /locus_tag="OO544_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HCR6769575.1"
                     /translation="MTQNYELIVKGIRNFENKVTVTLALRDKKRFDGEIFDLDISLDR
                     VEGAALEFYEAAARRSIRQVFLDVAAGLCEGDELLPETRPCSEARYTIKINSSDNSIT
                     GC"
BASE COUNT          778 a          762 c          885 g          750 t
ORIGIN      
        1 cttccaggcc catccgctcc ggtgggcctt tttactgcag aaaacaggtt ccccgttaaa
       61 tgctatgttg ctcacaattc agtaagttga cagttgcctg tcagactggg catttgttaa
      121 aaaaatttcg catggtgaat ccccctgagc ggaggggcga ctggtgacgg tataatctct
      181 gattatcaaa acgagaatga cgcgggttta gtggcaccgg gctgaactca ccgggaggca
      241 cccggcacca tgtgcatgat gatacagata cgcggcttta gcccctctcc ggaggggttt
      301 tcttgtgggc aaaaaaagcc cgcgctggga gacgcgggcg gcaaggaata aacaataaaa
      361 cgtgaagtaa tatttcagct ggcgaataat accccatagt aatcactctg cgcaactgcg
      421 cggtcttttt cgaattgcgg gctgtcgtct ctcttctgcc attgtcctgt aacttccgga
      481 cttcagcccg ctccttattt tactcacaat attatcccgg ccgggaggat tcatggcatt
      541 taaacactat gacgtggtca gggcggcatc gccgtcagac cttgcgaaac gactgacaca
      601 aaaactgaag gagggctggc agccgtttgg tagtccggtg gccataaccc cttataccct
      661 gatgcaggcg attgcagcag aaggtgatgt ggtcgtcagt ggtgcaactg agccggagtg
      721 gtactacgtc atcgtactgg ccgggcaatc caatgccatg gcttacggtg aaggtcttcc
      781 gcttccggat tcatacgatg cgccccatcc gcgcattaag caactggccc gtcgtaacac
      841 agtgactccc ggtggtgaag tatgcgtatt taacgacatc attcctgctg accattgtct
      901 gcatgatgtt caggatatga gtacgattaa ccatccccgg gctgacctga gcaaagggca
      961 gtacggctgt gtcggacagg gcttacatat tgccaaaaaa ctgcttccgt atatccctaa
     1021 taatgcgggg atcctgctgg taccatgctg tcgtggtggt tcggcattca cccagggcac
     1081 ggaggggaca ttcagcgagt ccacgggggc cagtcaggat tcggctcgct ggggagtggg
     1141 taagccgtta tatcaggatc tgcttttccg cacgaaggca gcattgcaga aaaacccgaa
     1201 aaacgttttg ctggcgatat gctggatgca gggggaattc gatatgacga atgccagtta
     1261 cgcccagcag ccagcagcat ttcttgcaat ggtacagcag ttccgtgctg accttgccgg
     1321 gctggcggcg cagtgtcacg gtggaagtcc ggcatcagtc ccctggattt gtggcgacac
     1381 gacatacgcg tggaaacaag aacacggtac gcaatatgaa gtggtatatg gtgcatataa
     1441 aggtaaagaa tcccagcaga tttattttgt tccctttatg accgatggta gcggagttaa
     1501 tacaccgaca aacaacccgt cagaagatcc tgatattgcc gggtctggtt attacggttc
     1561 ggcatcccga acgaacaaaa actgggtatc atcaaatcgc ccgacgcatt tcagctcatg
     1621 ggcgcgtcgt ggcattattc ccgatcgtat ggcaactgct attctgaacg tagccggtcg
     1681 caccttagcc ttcattagtg gtaaggcacc ggaaatcaaa ccctcgcccg gcggcgacac
     1741 gccatcgggg ccgtctgaag atgcatccat acgcacaatc tccctgttgc cgacagccgg
     1801 agacgctgct gcgcagggct ggagcattaa gaatggcgga gttcagttgt cagatggtgt
     1861 atttaagatc accaagcaga gcaataaagc ctggtccctg acgcgcccgg tggatgacgc
     1921 agtctccctg ctgacacggg gtggcagact gagctgtaag tttcgactgt caggcgcact
     1981 gaccaacaac cagttcggtc tgggaattta tctgtatacc gatgtagcgt tacctgacgt
     2041 cgtggcgatg accgggactg gtaacccgtt cctgatgtcg ttcttcaccc agaccacaga
     2101 cggcaaactg aatctgatgc atcacaagaa agcaggaaac acaaagttgg gcgagttcgg
     2161 gaattacagt aacgactggc tgacgctgga gctggtgttc accgccggca gtgccacggt
     2221 tactccgaaa ctgaatggag tggctggccc ggcattccag gtcataaaag acagtctgac
     2281 actggggctg aatgcgctga cgctgacgga tattaccaaa aatgcagcgt atggcgttga
     2341 gatagaaagt ctggtgctgg agataaatgc accggcatca tcataaaaag tgagccagcc
     2401 aaatggaagg tatcgttaaa ctcaccggta gtgtcagtgg gtcgtctgag atgcctgcat
     2461 gagttatcag agccatcagt acttaactgg tggctttttt tattgttgtc agcttccgga
     2521 taacgggaga cggggtatgt accagatgga aaaaatcaca acaggtgtgt catacaccac
     2581 gtcagcggtg ggaacgggct actggttcct gcagttgctg gacagggttt ccccgtctca
     2641 gtgggcggca ataggcgtgc tggggagtct gctgtttggg ctgctgacat atctgactaa
     2701 cctgtatttc aaaatcagag aggaccgtcg taaggctgca cggggagagt aattcaatga
     2761 ctcaaaacta tgaactgatt gtgaaaggga tccgcaattt tgagaataaa gttacggtaa
     2821 ctttagcgtt acgggacaaa aaacgctttg acggtgaaat ttttgacctg gacatctcgc
     2881 tggaccgtgt tgaaggtgcc gcgctggagt tttatgaggc agcagccaga aggagcatca
     2941 gacaggtctt cctggatgtt gctgccgggt tatgtgaagg ggacgagctg ttgccagaaa
     3001 cgcgcccctg ttcagaggcg cggtatacca taaaaattaa cagttctgat aactcgatta
     3061 cgggttgtta gctttttgca gttggctttc cagtatcttt cattggtagc atcctgataa
     3121 atatccatga gcgcaaaaat caaatacggc ctgtcagctg ctgttctggc gctga
//
LOCUS       DAJTZL010000293         3154 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.296, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000293 DAJTZL010000000
VERSION     DAJTZL010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3154
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.296"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(61..372)
                     /locus_tag="OO544_004118"
                     /pseudo
     CDS             complement(61..372)
                     /locus_tag="OO544_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203750.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase"
     gene            complement(369..755)
                     /gene="trbI"
                     /locus_tag="OO544_004119"
     CDS             complement(369..755)
                     /gene="trbI"
                     /locus_tag="OO544_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TrbI"
                     /protein_id="HCR6769576.1"
                     /translation="MTTTQKTTDVTAPRRSHWWWTVPGCLAMVLLNAAVSYGIVRLNA
                     PMTAAFNMKQTVDAFFDSASQKQLSEAQSKALSARFNTALEASLQAWQQKHHAVILVS
                     PAVVQGAPDITREIQQDIARRMRAEP"
     gene            complement(752..2002)
                     /locus_tag="OO544_004120"
                     /pseudo
     CDS             complement(752..2002)
                     /locus_tag="OO544_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069745.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
     gene            complement(2004..2186)
                     /locus_tag="OO544_004121"
                     /pseudo
     CDS             complement(2004..2186)
                     /locus_tag="OO544_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490565.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2208..2519)
                     /gene="traL"
                     /locus_tag="OO544_004122"
     CDS             complement(2208..2519)
                     /gene="traL"
                     /locus_tag="OO544_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HCR6769577.1"
                     /translation="MLGDENKLKKYRFPETLTNQSRWFGLPLDELIPAAICVGWGITT
                     SKYLFGIGAAVLVYFGIKKLKKGRGSSWLRDLIYWYMPTALLRGIFHNVPDSCFRQWI
                     K"
     gene            complement(2534..2884)
                     /gene="traA"
                     /locus_tag="OO544_004123"
                     /pseudo
     CDS             complement(2534..2884)
                     /gene="traA"
                     /locus_tag="OO544_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673167.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system pilin TraA"
     gene            complement(2930..>3154)
                     /locus_tag="OO544_004124"
     CDS             complement(2930..>3154)
                     /locus_tag="OO544_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6769578.1"
                     /translation="DSYDNAMAEIIKGLYKAEVIHRKSWKNRTEVELATLTWVDWYNN
                     RRLLERLGHTPPAEAEKAYYASIGNDDLAA"
BASE COUNT          726 a          909 c          772 g          747 t
ORIGIN      
        1 gtacacctgc acatgctgct tcatacgcga cagggccacg taggctgact caaagccggc
       61 cacccgccct tcgttatccc ggatatccgc agccagtctg acggacgggt caaacaaccg
      121 gctgctgtat ttctccgtgc gtccgatacc gggcacggca gggggccgca gactgttgcg
      181 gatcacccgc tccttaaagg catccatttt ccggcccatc tcaccggact gctccagtgc
      241 cgtcaggcgc tgcatgatca ccgtcagcat gtccggctcc tttaccggcc acagatcacc
      301 ccaggtgcca agatcggcgg cggccacact ctgcccccat atcagcaggg caatcagccc
      361 ccggcatctc atggttccgc tctcattcgc cgggcaatat cctgctggat ttcccgggtg
      421 atatccggag ccccctgtac cacggcaggc gacaccagaa tgaccgcgtg gtgtttctgc
      481 tgccacgcct gcaggctggc ttccagtgcc gtgttaaaac gcgctgaaag ggcttttgac
      541 tgtgcttctg acagttgttt ctgactggcg ctgtcaaaaa atgcatccac agtctgtttc
      601 atgttgaagg cggcagtcat cggtgcgttc agtctgacaa tgccgtaact gacggctgcg
      661 ttcagtaaca ccattgccag gcatcccggc accgtccacc accagtggct gcgtcggggg
      721 gctgtaacat cagtcgtttt ttgcgttgtg gtcatgcaac actcctgtat ttctcatgtt
      781 cttccagcca ggcttccagc gaagccatct ccggccctga cttcttccac gccagctgcc
      841 acaccgcctc atgaatactc agcccctctt tacgtttctg ctgcacaaac tcaaaatccg
      901 ggccgtcaga gctgtacatg gcgcggctta acgggtccac aaacaggcgg tgccaggagg
      961 aatggttttc cacctgcagc aggaaggaac tgaaccactg gtctttggcc gcaccaaact
     1021 taccaatcat gtcgcgctgc agtggctgga actggtccgg atacagctgg ttgtattttg
     1081 cgaactcctt cgcactctgt ttgaggataa ttttgtagga ggagttaccc catgccgcac
     1141 gggccgcact ggaggccttg tcagagtcaa agtcgacgat gttctgtgtg atggtgatat
     1201 aagcaccggt atgacgacgg gcggtacggt aacctttctc aataaactca ccgaccttgt
     1261 gatttttgaa gtccagcaga cgccagcctt catcaatgac gttcagtttc ttgaggttac
     1321 gcggcgtgcg gtacatcctg ttctcgatgt agataatcag ggagaacatc accgcaacca
     1381 gcagtgacgg acggtcttcc agtccgccca gctccagcac caccattttt gcgtcatccc
     1441 gcagggacgg ctcatcagag ttaaaatact ggccgtaagt gccgttggca gtgtactggt
     1501 caagcagcac aatcatttcg tccagacggc tgcggatagt cggtgacccg gcatactgct
     1561 cgctgtcact ggcgtttttc aggaaatcca ccacgtcatc aatacgtgcc ctgttctctt
     1621 tggccagcca ggaggccctg accgcctgca gcagcagacc ttcatgcact tcatccaggt
     1681 taccgttggg gctggccatc actgacaact ggtcacggac acgctccgct gactggtcga
     1741 tatcggtgat gttcgcaaac gggttaaaac gcagggtttc accgtccaga tatacgccac
     1801 ccatgttctc acacagggac ttgtagccat cccccatgtc gaacaccacg gcaaagcccc
     1861 cggagtccag cacgctgcgg ataagtggct gtatcagccc ggttttaccg gccccggagg
     1921 tgccacagac cgccatgttg tagttggtgt tattcattcc ccggaagaaa atatcgataa
     1981 acgccagctg gttacggtag gtgtgacacg gcaaagggca cattaaatcc cattggggtc
     2041 acggcagtgc gttgctcatt ctgaagacgg tagttattca ccccctgaat gaggttaaca
     2101 gagaggctga gaacaataag cactgacata aatataaagg cgatggccat tacacgactg
     2161 gtacttaaac gggcaccgtg ttccatataa caatcctggt atcagttcta tttaatccac
     2221 tgccggaaac acgaatcggg aacattatga aaaataccgc gcagcagggc tgttggcata
     2281 taccagtaaa tcaggtcacg taaccaggaa ctgccccgcc cttttttcag ttttttaatc
     2341 ccgaaataaa ccagaaccgc tgcaccaata ccgaacagat atttcgatgt tgtgataccc
     2401 cagccaacac agattgctgc ggggatcagt tcatccagtg gcaggccaaa ccagcggctc
     2461 tggttggtca gtgtttccgg gaaacgatat ttcttaagtt tattctcgtc tcccaacatc
     2521 gttttatttc ctgtcagaat ccggcaacag acatacctac tgcaataaat acagagagta
     2581 tggcaaagcc gaccaggaac ttgacgtttt tggtcatcat gtacatgact gcaccgacca
     2641 gaacttcagc cagaacaacc catttaacga tactggagtc cttaccgaag gtcgccttaa
     2701 ccgtttcctg acctttagcc atcaggtccg tgcctttacc agcagccata gctaactcag
     2761 ggaacaacat catcagaaca gcaaccggga tcactgcacg aaccaggcga agcatattca
     2821 gacgagtgaa tttagaaaaa aacgatttct ttttgacggg cgcagaagta ccctgaacac
     2881 ttaacgctga accgccccgg gtttcctgga gagtgtttta tctgtgaact caggctgcca
     2941 gatcatcgtt tccgatggaa gcataataag ctttttctgc ttctgccgga ggagtatggc
     3001 ccagcctttc cagcaatcgt cgattgttat accagtccac ccacgtgagt gtggccagct
     3061 ccacttctgt ccggtttttc cagctcttac ggtgtattac ctccgctttg taaaggccct
     3121 tgatgatctc tgccatcgcg ttgtcatacg agtc
//
LOCUS       DAJTZL010000294         3109 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.297, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000294 DAJTZL010000000
VERSION     DAJTZL010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3109
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.297"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..1255)
                     /locus_tag="OO544_004125"
     CDS             complement(<1..1255)
                     /locus_tag="OO544_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HCR6769579.1"
                     /translation="MAQFKVDDFLIELSFNSQKVLKGLEKAEKQTMQVASRIEKRLNK
                     SFRVNPTPLNDSLKLMERNVDKTVSKIEQRLKNTRAFKIKTEIEDTLKPLRQPRQPRI
                     SGSRAITAAYSANMSKLRDFNPMLEKYFKSQYYSLSSKSKTLGNETFNRELAKLNQSL
                     RETLSKFNKTTSKNNHSENASNSLDVLATSAIKAGTAIYSFQTALEAYRRIMEVGLKK
                     EASQRAAQFVLGDEGAKRATEFVKNLADSSGVDQIETLSSFAKFSAGAGDMNADQKES
                     LFSNVIGTSRLMGLSTDEINGILKAFEQMASKGKIQAEELRGQLGDRMAGAFQLFARS
                     LGMTTEELDKAMKDGKVLSKDVFPKVSAEMGRMIDKAGGWEKIINSTQTQLGRLSNSW
                     NNNLALMFDGSQEGLTDFTRSLTNLL"
     gene            complement(1412..1621)
                     /locus_tag="OO544_004126"
     CDS             complement(1412..1621)
                     /locus_tag="OO544_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6769580.1"
                     /translation="MFEERLETIRSVCENLKLQTKPALRIKNKRQVITSHKPKTRKIP
                     KWCIDRIPSDAQIIGETELHYLVRH"
     gene            complement(1644..2354)
                     /locus_tag="OO544_004127"
     CDS             complement(1644..2354)
                     /locus_tag="OO544_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6769581.1"
                     /translation="MTNDLYINGKKVDSFGDWSPQGEHPLHTIQRQEHDKRIELEQEL
                     KMSPKVISFVSPEPQEMPDICKTEALFELEREYYPALKSQRLRLDVAYDAVKNFEETS
                     KPSEYDIAYEIKSNPFIYYEGNFNDGFGTAIEDVPKVLQSIPEGFRLIDVRQALRGSG
                     QFILMTDKTDEEILQLAEANLTKHYSGKLDKLKQKLKKELDTMKELVSEYESQKKIAL
                     MADIEQLTKISQKYSKAV"
     gene            complement(2870..>3109)
                     /locus_tag="OO544_004128"
     CDS             complement(2870..>3109)
                     /locus_tag="OO544_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6769582.1"
                     /translation="DLDKLLKLMAENSEEIEDFIQEIETGEPRFMFPVTDSNMKRLII
                     EETNKYSVSPEKYLKAAIAILYSDNILVTDSKVVH"
BASE COUNT          911 a          654 c          507 g         1037 t
ORIGIN      
        1 taagtaaatt agtcagtgac cttgtaaagt ctgttaaacc ttcctgtgaa ccatcgaaca
       61 ttaaggccag attattattc catgaattac ttaagcggcc taactgcgtc tgagtagagt
      121 taataatctt ttcccatcca ccagctttat caatcatcct tcccatttca gcggatactt
      181 taggaaaaac atctttagaa agaaccttac cgtctttcat ggccttgtct aattcttccg
      241 tagtcatccc aagacttctg gcaaataact gaaaagcacc tgccattctg tcacctaatt
      301 gaccgcgtag ctcttcagcc tgaatctttc ctttagatgc catctgctcg aaagctttta
      361 aaatcccgtt aatttcatcg gtgcttagcc ccattaatct ggatgtacca atcacattag
      421 aaaataagga ttctttctgg tctgcattca tatcaccagc accagcggaa aacttagcaa
      481 aactgcttaa tgtttcaatc tggtctacac cactactatc agcaagattc ttaacaaatt
      541 ctgtagctcg tttagcacct tcatcaccta aaacgaattg tgccgctcgt tgtgatgctt
      601 cttttttcag accaacctcc ataatccgcc tgtaagcttc taaggctgtc tgaaaactat
      661 aaatggctgt acctgctttg attgcactcg tagctaatac atccagacta tttgaagcat
      721 tttcactgtg attatttttg ctggtggtct tgttaaactt agaaagtgtt tcacgaagag
      781 actgatttaa ctttgctaac tcacgattaa aagtttcgtt tccaagtgtc ttggatttac
      841 tgcttaagct gtaatactgg cttttaaaat acttctcaag cataggatta aagtcacgaa
      901 gcttgctcat attcgctgag taagctgctg tgattgctct gcttccgcta attcgtggct
      961 gtcttggttg tctaagtggc tttaaagtat cctcaatttc agttttaatc ttaaacgctc
     1021 tagtgttttt aagacgctgc tcaatcttgg aaacagtttt atcgacattt ctttccatta
     1081 gtttgagaga atcatttaaa ggcgtaggat taactctaaa cgacttattc aatcgcttct
     1141 caattcgtga tgctacctgc atcgtttgtt tctcggcttt ttccaagcct tttaaaactt
     1201 tctgagagtt aaaacttaac tcaatcagaa aatcatcaac tttaaattgt gccattcata
     1261 tttttcacct ctttaagcgt gttgtacgta aattctattt ttcagtgtgg gtattagctt
     1321 tacagatttc acgtagcaca catacaaaaa aagggagagc taccgatgtc actcgataat
     1381 ctctccccgt atttacccaa ccaattcttt tttaatgtcg gacaagataa tgtaattccg
     1441 tttcacctat gatttgagca tcagaaggaa tacggtcaat acaccacttt ggaatcttgc
     1501 gtgtttttgg tttgtgtgag gtgataactt gacgtttgtt tttaattctt aaggctggct
     1561 tagtttgtaa ctttaagttt tcacaaacag accgaatagt ctctaaacgt tcttcaaaca
     1621 ttaatcacct ctttaattca taatcaaaca gccttgctat acttctgaga aatcttcgtt
     1681 aactgctcaa tatcagccat caaagcaatc ttcttctgcg actcatattc actaacaagt
     1741 tctttcatag tatcaagttc ttttttaagc ttttgcttta acttatccag tttaccggaa
     1801 taatgcttag tcaaattagc ttcagctaac tgaagaatct cttcatctgt tttatcagtc
     1861 atcagaataa attgaccaga accccttaat gcttgtctga catcaattaa ccggaaccct
     1921 tcagggatac tttgaagaac tttaggaacg tcttcaatcg ctgtaccaaa accatcatta
     1981 aagtttcctt cataatagat aaacgggttt gacttaatct cgtaagcaat atcatattct
     2041 gaaggtttcg aggtttcttc gaagttctta acagcatcgt aagcgacatc aagtctaaga
     2101 cgctgtgatt ttaaagctgg gtaatattca cgctcaagct caaatagtgc ttcagttttg
     2161 cagatatcag gcatttcttg aggctcagga gacacaaagg aaattacctt tggtgacatc
     2221 tttagctctt gctcaagctc aatgcgcttg tcatgctctt gacgctgaat agtatgtagc
     2281 ggatgttcac cctgtggtga ccagtctccg aagctatcaa cctttttacc gttaatatat
     2341 aaatcgtttg tcattataat ttcctctaag tgtggttatt tcttaataga gcaccttcaa
     2401 cgccaataca cttagaagat gacttaacga tttattaaag atgctctatt aagaaaagcc
     2461 ctaaacataa tctgtgaagg gctttggagg tttgcttata acgttaacga agtttcagga
     2521 aaactttctt taaaagctct gtttaaaaac tcttaaagaa agccctaact catgattagg
     2581 gcaaagctgt aaaggattca ataatgaatg aacaaagact ttttgagata gcctctcaga
     2641 ccaacctgaa aggctaggac gaaaagcatt gcacctttcc aactcagaat atccattcat
     2701 cggattattg aaagaaaaaa taagagagaa ttgctctccc ccatatatac aattatgtca
     2761 tgctcaaggt tgtttgtcaa cagcctttaa cagattggta acatttatac tttaatagat
     2821 gcacctgcaa tcataccaat caatacaaaa cagataatca ccaattccat taatgcacca
     2881 ccttcgaatc cgtcaccaga atattatcgc tatacaaaat tgctatagcc gccttgagat
     2941 atttttcagg gctaacgctg tatttgttcg tttcttcgat aataagacgc ttcatgtttg
     3001 agtcggtaac aggaaacata aaacgcggtt caccagtttc aatttcctga ataaagtctt
     3061 caatttcttc tgagttttca gccattagct ttagtaattt atcaaggtc
//
LOCUS       DAJTZL010000295         3045 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.298, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000295 DAJTZL010000000
VERSION     DAJTZL010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3045
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.298"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..172
                     /locus_tag="OO544_004129"
     CDS             <1..172
                     /locus_tag="OO544_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6769583.1"
                     /translation="AKALMAQYRVPVVVEVILERVTNISMGSELDNVMEFEDIADNAV
                     DAPTETCFMHYE"
     gene            185..1036
                     /gene="hyi"
                     /locus_tag="OO544_004130"
     CDS             185..1036
                     /gene="hyi"
                     /locus_tag="OO544_004130"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000943555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HCR6769584.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKKI
                     NCLVGKTPAGFSSEQIHATLVENLRYAANMLMKEDILLLIEPINHFDIPGFHLTGTRQ
                     ALKLIDDVGCCNLKIQYDIYHMQRMEGELTNTMTQWADKIGHLQIADNPHRGEPGTGE
                     INYDYLFKVIENSDYNGWVGCEYKPQTTTEAGLRWMDPYFLNRVVATGKVMKPVLAQR
                     QKVVSPP"
     gene            955..2133
                     /gene="allB"
                     /locus_tag="OO544_004131"
                     /pseudo
     CDS             955..2133
                     /gene="allB"
                     /locus_tag="OO544_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
     gene            2190..>3045
                     /locus_tag="OO544_004132"
     CDS             2190..>3045
                     /locus_tag="OO544_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
                     /protein_id="HCR6769585.1"
                     /translation="MFNFAVSRESLLSGFQWFFFIFCNTVVVPPTLLSAFQLPQSSLL
                     TLTQYAFLATALACFAQAFCGHRRAIMEGPGGLWWGTILTITLGEASRGTPINDIATS
                     LAVGIALSGVLTMLIGFSGLGHRLARLFTPSVMVLFMLMLGAQLTTIFFKGMLGLPFG
                     IADPYFKIQLPPFALSVAVMCLVLAMIIFLPQRFARYGLLVGTITGWLLWYFCFPSSH
                     SLSGELHWQWFPLGSGGALSPGIILTAVITGLVNISNTYGAIRGTDVFYPQQGAGNTR
                     YRRSFVATG"
BASE COUNT          695 a          711 c          836 g          803 t
ORIGIN      
        1 ggcgaaagcc ttaatggcgc aatatcgggt accggtagtc gtggaagtta ttcttgagcg
       61 tgtgaccaat atttcgatgg gcagcgaact ggataacgtc atggaatttg aagatatcgc
      121 cgataacgca gtggacgcac cgactgaaac ctgcttcatg cactatgaat aagggagata
      181 aataatgtta cgtttctctg ctaatttatc gatgttattt ggtgaatatg attttctcgc
      241 ccgttttgag aaagctgcgc agtgtggttt tcgcggcgtt gaatttatgt ttccttatga
      301 ctacgacatt gaagaattaa aacaggtgct ggcgagtaat aaactcgaac atacgctgca
      361 caatctaccg gcgggtgact gggcggcggg ggagcgcggt attgcctgta ttcctggccg
      421 tgaagaagag tttcgggatg gcgtagcagc agcgattcgt tatgcccgtg cgctggggaa
      481 taaaaaaatt aactgtctgg tcggtaaaac gccggctggt ttcagcagtg aacagattca
      541 cgcaacgctt gtagaaaacc tgcgttatgc cgcgaatatg ctgatgaaag aagatatttt
      601 attactgatt gaacctatta accattttga tattcctggt ttccatctca ccggaactcg
      661 gcaggcgctg aaattgattg atgatgttgg ttgctgcaat ttgaaaattc agtatgacat
      721 ttatcacatg cagcggatgg aaggtgaatt aaccaacacc atgactcagt gggccgataa
      781 aattggtcac ctgcaaattg ctgataatcc gcatcgcggc gaaccgggga ccggagaaat
      841 taattatgat tatctcttta aggtaatcga aaattctgac tacaacggtt gggttgggtg
      901 tgaatataaa ccccaaacca ccacggaagc cggtttacgc tggatggatc cgtactttct
      961 gaaccgggtc gtagccactg ggaaggttat gaaaccggta ctcgcgcagc ggcaaaaggt
     1021 ggtatcacca ccatgatcga aatgccgctc aaccagctgc ctgcaacggt tgaccgcgct
     1081 tcaattgaac tgaagttcga tgccgctaaa ggcaagctga ctatcgatgc ggcacaactc
     1141 ggtggcctgg tgtcttacaa catcgaccgt ctgcatgagc tggatgaagt gggcgttgtc
     1201 ggcttcaaat gcttcgttgc gacctgtggc gatcgcggta tcgacaacga cttccgtgat
     1261 gtaaacgact ggcagttctt caaaggtgcg cagaagctgg gcgaactggg gcagccggtg
     1321 ttggtgcact gcgaaaacgc gctgatctgt gacgcaccgg gcgaagaagc gaaacgtgaa
     1381 ggtcgcgtaa ctgcccatga ctatgtggct tcgcgtccgg tatttaccga agtggaagcg
     1441 attcgccgcg tgctgtatct ggcgaaagtt gccggttgcc gtctgcacgt ttgccatatc
     1501 agcagcccgg aaagtgttga agaagtgact cgtgcacgtc aggaaggtca ggatgttacc
     1561 tgtgaatcct gcccgcatta ctttgtactg gataccgatc agttcgaaga aatcggtact
     1621 ctggcgaagt gttcaccgcc gatccgcgat ctggaaaacc agaaaggcat gtgggaaaaa
     1681 ctgtttaacg gtgaaatcga ctgcctggtt tccgaccact caccatgccc tccggaaatg
     1741 aaagccggca acatcatgga agcatggggc ggtattgccg gtctgcaaaa ctgtatggac
     1801 gtgatgttcg atgaagcggt acagaaacgc ggaatgtctc tgccaatgtt cggcaaatta
     1861 atggcgacta acgcagcaga tattttcggt ctgcagcaaa aaggccgtat cgccccagga
     1921 aaagatgccg acttcgtctt cattcagccg aatagcagct atgttcttac caatgacgat
     1981 ctggaatatc gccacaaagt cagcccgtat gttggccgta ccattggcgc gcgtatcacg
     2041 aaaaccatct tacgtggtga tgtgatttac gacattgaac agggcttccc tgttgcgccg
     2101 aaaggtcaat ttatccttaa acatcagcag taatctggcc cctgcaatgc ccgtccttgc
     2161 ggcgggcatt ctccggttaa ggtgtgttta tgttcaattt tgcagtcagc cgcgaaagcc
     2221 tgttatcagg atttcagtgg tttttcttta ttttttgcaa cacggttgtg gttcctccta
     2281 cgctactttc tgcttttcag ttgccgcaaa gtagcctgct tacgctcacg caatatgctt
     2341 ttcttgctac cgcactggcc tgcttcgctc aggcgttctg cggtcatcgt cgcgctatta
     2401 tggaagggcc gggtggcctg tggtggggaa ccatccttac tatcaccctt ggcgaagcat
     2461 cgcgcgggac accgatcaac gatatcgcta ccagtctggc ggtggggatt gcactctccg
     2521 gcgtgctgac gatgttgatt ggttttagcg gattaggcca tcgcctggca cggttattta
     2581 cgccgtcggt gatggtcttg tttatgttga tgctgggcgc acagctgacc actatctttt
     2641 tcaaaggtat gctcgggctg ccgtttggca tagccgaccc atattttaaa attcagttac
     2701 caccgttcgc gctctcggtg gcggtgatgt gcctggtact ggcgatgatt atctttctgc
     2761 cgcaacgttt tgcccgttat ggcctgctgg tcggcaccat aaccggctgg ttgttgtggt
     2821 acttttgctt tccttcttcg cattcgctct ccggtgagtt gcactggcag tggttcccgc
     2881 tcggcagtgg cggtgctttg tcgccgggaa ttattctgac ggcggtgatt acaggtctgg
     2941 taaatatcag caatacctac ggtgcgattc ggggcacgga tgttttttat ccgcagcagg
     3001 gcgcggggaa tacgcgttat cgtcgcagct ttgtggcgac cggat
//
LOCUS       DAJTZL010000296         3010 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.299, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000296 DAJTZL010000000
VERSION     DAJTZL010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3010
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.299"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            63..1226
                     /locus_tag="OO544_004133"
     CDS             63..1226
                     /locus_tag="OO544_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001416350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR6769586.1"
                     /translation="MPNHDRFECQTAIDSDAILQLAWAVFKIFRIPVNQWTLATAALG
                     GVFLVSGLILLMNYNHPYTFTAQKAVIAIPITPQVTGIVTEVTDKNNQLIQKGEVLFK
                     LDPVRYQARVDRLQADLMTATHNIKTLRAQLTEAQANTTQVSAERDRLFKNYQRYLKG
                     SQAAVNPFSERDIDDARQNFLAQDALVKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQ
                     LTEAKYNLEQTVIRAPSNGYVTQVLIRPGTYAAALPLRPVMVFIPEQKRQIVAQFRQN
                     SLLRLKPGDDAEVVFNALPGQVFHGKLTSILPVVPGGSYQAQGVLQSLTVVPGTDGVL
                     GTIELDPNDDIDALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(1686..2123)
                     /locus_tag="OO544_004134"
     CDS             complement(1686..2123)
                     /locus_tag="OO544_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6769587.1"
                     /translation="MADKAILWALISASTKEGRKACSLSYFACKAAEAELGLAYMAAN
                     DNKEFLTSLSNIMRYKIDAGLSESYTCYLLSKGKIIRPYLKNLNPLQLAADCIETVNK
                     IKDKNKKIIDINSVNICSDDKNIKLRVNSTIMAIDDSIKCIDE"
     gene            complement(2212..>3010)
                     /locus_tag="OO544_004135"
     CDS             complement(2212..>3010)
                     /locus_tag="OO544_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000015397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCR6769588.1"
                     /translation="QMQNQMDPVYTPARKIHLYHCDHRGLPLVLISTEGATEWCAEYD
                     EWGNLLNEENPHQLQQLIRLPGQQYDEESGLYYNRHRYYDPLQGRYITQDPIGLKGGW
                     NFYQYPLNPITDIDPLGLATCLYSITLSMLSCVSDTQNDDNSYDVLTIPVASGNNGNN
                     MQCKNNPGCTHLQNRGPIPQGVWSWNVNGPGATNRKPNGIRLVPSANTETYNRDGFLT
                     QSCLNAFGPSLGPRFCSEGCITGSSNDMQKLNELIFSEPDSTLTVTD"
BASE COUNT          794 a          649 c          674 g          893 t
ORIGIN      
        1 tcaatgatcg gaagtatgcc aaatcgtgat cgattgtgat gccaaactgt gatcggagat
       61 ggatgccaaa tcatgatcga ttcgaatgcc aaactgcgat cgattcagat gccattttgc
      121 agctggcgtg ggcggtcttt aaaatctttc gtattccggt gaatcagtgg acgctggcga
      181 cggcggcgct gggaggcgtg tttctggtga gtggtttgat tttgttgatg aactacaacc
      241 acccttacac ttttaccgcg caaaaggcag tgatagcgat ccctatcacg ccacaggtga
      301 cgggaattgt tactgaagtc actgacaaga ataatcagct tattcaaaag ggcgaggtgc
      361 tttttaagct cgacccggtt cgttaccagg cgcgagttga cagacttcag gctgacctga
      421 tgacggcgac gcataatata aagacgctgc gtgcgcagct cactgaagcg caggccaaca
      481 ccacccaggt ttcagcggag cgcgaccgtc tgtttaaaaa ttatcaacgt tacctgaaag
      541 gcagccaggc ggcggtgaat ccgttctcgg aacgtgacat cgacgatgcg cggcaaaatt
      601 tcctcgcgca ggatgcgctg gtgaaaggct cggtggcgga gcaggcgcag atccagagcc
      661 agctcgacag tatggttaac ggcgagcaat cgcagattgt gagcttaaga gcgcaactta
      721 ctgaagcaaa atataacctt gagcagactg tcattcgcgc gccgagcaat ggctacgtta
      781 ctcaggtact gatccgccca ggtacatacg cagctgcctt gccgctgcgt ccggtgatgg
      841 tcttcatccc cgagcaaaaa cggcaaattg tcgcccaatt tcggcaaaac tcgctgttac
      901 gtctgaaacc tggcgatgat gcggaagtgg tgtttaacgc gctacctggg caggtgttcc
      961 acggcaaatt gactagtatt ttacctgtcg tgccaggcgg ttcttatcag gcgcaggggg
     1021 tattgcaatc attaacggtc gttcccggca cggacggcgt gctgggaacc attgaactgg
     1081 accctaacga tgatatcgat gccttacccg acggcattta cgcccaggta gcagtttact
     1141 ccgaccattt cagccatgtt tcggtgatgc ggaaggtgct gctaagaatg accagttgga
     1201 tgcattattt gtatttggat cattgagggg gaagggggag attatggaac tttgctccct
     1261 gcaagggcta attatttttg catattactt cattggctgc gatgattttt ctttaatatt
     1321 cattatgtta ataagttctg gatgagtgtg ctctcattgg attttttatc cgtcttgttc
     1381 tgccgcagga tgagactata catcgtcgag atcgcaatga tgatatgagt tgttcctcct
     1441 gcgacggctc taatcaaaaa agagcaggag tgcttttcca tctattgcga tgatggcttt
     1501 attatctgaa ggatgacagt cacatttaaa aaatatttaa tctatagatc acaatgtagg
     1561 gtcatggaat atatgtatac aactattttc ttcttcactt ttaattatcg tattcattat
     1621 aaaactaagt taaaaaaact cctagagagt gagttattaa tatattttta aataaaatta
     1681 agttatcatt catcgatgca ttttatagaa tcatcaatgg ccataatagt actattaact
     1741 ctcaatttta tatttttatc atcactacaa atattaactg aatttatatc aatgattttt
     1801 ttattcttat cttttatttt atttactgtt tcaatgcaat cagcggcaag ttggagtgga
     1861 tttagatttt tcagatatgg tcttatgatc tttcccttac ttaataaata gcatgtatag
     1921 gattcggaaa gtccagcatc tattttgtac ctcattatat tagataagga tgtgagaaat
     1981 tccttgttat cattcgcagc catatatgcc agtccgagtt cagcctctgc ggccttacag
     2041 gcaaaatagc ttagtgaaca cgcttttcgt ccctctttag tactggctga tattagtgcc
     2101 cataatattg ctttgtctgc caaatctaac tctctctgaa attgtcttgc agtaactgca
     2161 ctcgcagaaa tagagaaaaa aatacaatta ttaataactt tgccatattt tttagtccgt
     2221 aactgttaat gtgctatctg gctctgaaaa tattaactca tttaattttt gcatgtcatt
     2281 agatgaacca gttatacaac cctcggaaca gaatcgaggt cctaaagacg gaccgaaagc
     2341 gtttaaacaa ctttgtgtta aaaatccatc acgattatat gtttctgtat ttgctgacgg
     2401 tactaaacgt ataccattag gttttctatt ggttgctcca ggaccattaa cattccagct
     2461 ccatacccct tgaggtattg gacctctatt ttgtaagtgt gtacaaccag gattattttt
     2521 acattgcata ttattaccat tattaccaga ggcgacagga atagtaagca catcataaga
     2581 gttatcatca ttttgcgtat ctgaaacgca tgaaagcata cttaaagtta tgctgtataa
     2641 acaggtcgct aatcccaacg ggtcaatatc agttatcgga ttcaacggat actgataaaa
     2701 attccatccc cccttcagtc caatcggatc ctgagtgata tatcgcccct gcagcgggtc
     2761 ataatagcgg tggcggttgt aatacaggcc ggactcctca tcatactgct gccccggcag
     2821 gcggataagc tgctgcagct gatgcgggtt ctcttcattc agcaggttgc cccactcatc
     2881 gtattctgcg caccattctg ttgccccttc cgtgctgata agcaccagcg gcaggccgcg
     2941 atggtcgcag tggtacaggt ggatttttcg cgccggcgtg tacaccgggt ccatctggtt
     3001 ttgcatctgc
//
LOCUS       DAJTZL010000297         2992 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.300, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000297 DAJTZL010000000
VERSION     DAJTZL010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2992
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.300"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            18..1076
                     /locus_tag="OO544_004136"
     CDS             18..1076
                     /locus_tag="OO544_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000035736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HCR6769589.1"
                     /translation="MAQAGIRPLVTLSHYEMPYGLVKNYGGWANRAVIDHFEHYARTV
                     FTRYQHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKACH
                     SLIPEAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFR
                     DHNITIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKS
                     SEWGWQIDPVGLRVLLNTLWDRYQKPLFIVENGLGAKDSVEADGSIQDDYRIAYLNDH
                     LVQVNEAIADGVDIMEYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTRKK
                     SFGWYAEVIKTRGLSLKK"
     gene            1162..2778
                     /gene="bglH"
                     /locus_tag="OO544_004137"
     CDS             1162..2778
                     /gene="bglH"
                     /locus_tag="OO544_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HCR6769590.1"
                     /translation="MFRRNLITSAILLMAPLAFSAQSLAESLTVEQRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSSAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAVVMMDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTNTIDLRYKDIPLWDKATLMVSGRYVTANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIASNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNDIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKFNESGYKTTLFHTFKVNTSMLTSRPEIRFYATYI
                     KALENELDGFTFEDNKDDQFAVGAQAEIWW"
     gene            2805..>2992
                     /locus_tag="OO544_004138"
     CDS             2805..>2992
                     /locus_tag="OO544_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6769591.1"
                     /translation="MNIKIAALTLAIASGISAQWAIAADMPASPAPTIPVKQYVTQVN
                     ADNSVTFRYFAPGAKNVS"
BASE COUNT          838 a          632 c          744 g          778 t
ORIGIN      
        1 atcggctgtt tgatgaaatg gcgcaggcgg ggatcaggcc gctggtaacg ttatcccatt
       61 acgaaatgcc atatgggctg gtgaaaaact acggcggttg ggctaatcgg gcggtcatcg
      121 atcacttcga gcattacgcc cgcacggtct ttacccgcta ccaacataaa gtagcgttat
      181 ggctgacgtt taatgaaatc aatatgtcgt tacacgcgcc attcacgggc gtggggctgg
      241 cagaagagag tggcgaggcg gaagtttatc aggcgatcca tcatcaactg gttgccagtg
      301 cgcgggcagt taaagcctgt catagcctga tccccgaagc gaaaatcggc aatatgcttc
      361 tcggtgggct ggtttacccc ctcacctgcc agccacagga tatgttgcag gccatggaag
      421 agaaccggcg ctggatgttc tttggtgatg ttcaggcgcg tggccagtat cccggctata
      481 tgcagcgttt cttccgcgac cacaatatca ccattgagat gactgaaagt gacgcagaag
      541 atttaaaaca tactgtagat ttcatctctt ttagttatta catgactggt tgtgtttccc
      601 acgacgaaag cattaataaa aatgcgcagg gcaacatact gaatatgatc cccaatccgc
      661 atctgaaaag ttcagagtgg gggtggcaaa ttgatccggt tggattacgg gttctgttaa
      721 atacgctttg ggatcgttat caaaaaccgt tatttattgt cgagaacgga ttaggcgcaa
      781 aagacagcgt tgaagcggat ggttcgatac aggacgatta tcgaattgcc tatttaaacg
      841 atcacctggt acaggtaaat gaagcgattg ccgatggtgt ggatattatg gagtacacca
      901 gttgggggcc aattgattta gtcagtgcat ctcattcaca aatgtctaag cgctacggct
      961 ttatttatgt ggatcgtgat gataatggcg aaggaagcct cacaagaacg cgtaagaaaa
     1021 gcttcggatg gtatgcagaa gtgatcaaaa cgcgggggct gtcattaaaa aaataaccaa
     1081 taaagcacct taattatcgt cgcattcaga acagtctgga tgcgatacgt taattctttc
     1141 tttgcaccat aaagggatat tatgtttaga cgaaatctta ttacctctgc catcttatta
     1201 atggcaccgt tagccttttc tgcacaatca ttggctgaat cattaacggt ggaacaacgc
     1261 cttgagttat tagaaaaagc gttaagagaa acgcaaagcg aactcaaaaa gtataaagat
     1321 gaagagaaga aaaaatatac gccagcgacg gtgaatcgta gcgtaagtac gaatgatcaa
     1381 gggtatgccg ccaatccgtt cccgaccagt agtgccgcaa aacctgatgc tgtactggtc
     1441 aaaaatgaag agaaaaatgc cagtgagaca ggttcgattt attcttccat gactctgaaa
     1501 gatttcagta agtttgtgaa agatgaaatt ggctttagtt acaacggcta ttaccgttct
     1561 ggttggggga ccgcctctca tggttcacct aaatcatggg cgattggttc tctgggccgc
     1621 tttggtaacg aatactccgg ctggtttgat ttgcagttaa aacaacgtgt ctacaacgaa
     1681 aacggcaaac gggttgatgc cgttgtgatg atggatggta acgttggtca gcagtactct
     1741 accggctggt ttggcgataa cgccggtggc gagaacttta tgcagttctc cgatatgtac
     1801 gttaccacca aaggtttcct gccctttgcg ccagaggctg atttctgggt gggtaaacac
     1861 ggtgcgccga aaattgaaat ccagatgctg gactggaaaa cgcagcgtac tgatgccgca
     1921 gcgggtgtag gtctggaaaa ctggaaagtc ggtccgggta aaattgatat cgcgctggtt
     1981 cgcgaagatg tcgatgatta cgatcgcagc ctgcaaaaca aacagcagat taataccaat
     2041 actattgatt tacgctataa agatatcccg ttatgggata aagcgacctt aatggtgagt
     2101 ggtcgttatg tcacggcaaa cgaaagcgca tcggaaaaag ataatcagga taataacggg
     2161 tattatgact ggaaagatac ctggatgttc ggcacatctt taacgcagaa atttgataaa
     2221 ggtggcttca acgaattctc cttcctggtt gcgaataact ctatcgccag taactttggc
     2281 cgttatgctg gcgcaagtcc atttaccacc tttaatggtc gttattatgg tgatcacacc
     2341 ggcggaacag cagttcgtct gacctcgcag ggcgaagcct atatcggcga tcattttatt
     2401 gtggctaacg cgattgttta ctccttcggt aacgatattt atagctacga aacaggcgca
     2461 cactctgatt tcgaatctat tcgtgcggtt gttcgcccgg cctatatttg ggaccaatat
     2521 aaccagacag gtgttgaact gggctatttc acccagcaaa acaaagatgc gaatagtaat
     2581 aaatttaatg agtctggtta taaaaccacg ctcttccata cctttaaagt caataccagt
     2641 atgttgacct cgcgtccgga aattcgtttc tacgccacgt atatcaaagc tctggaaaac
     2701 gaactggatg gcttcacctt tgaagacaat aaagacgacc agtttgctgt cggtgcgcag
     2761 gctgaaatct ggtggtaatg gaacttaatg aaaggaatat tacgatgaat ataaaaattg
     2821 ctgctttaac gctggctatt gccagcggta tttctgctca gtgggccata gcagcggata
     2881 tgccagccag cccggcaccc actattccgg ttaaacagta tgtgactcag gtcaatgccg
     2941 ataacagcgt gacctttcgc tactttgccc ctggggcaaa aaacgtctcc tt
//
LOCUS       DAJTZL010000298         2966 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.301, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000298 DAJTZL010000000
VERSION     DAJTZL010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2966
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.301"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..216
                     /locus_tag="OO544_004139"
     CDS             <1..216
                     /locus_tag="OO544_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HCR6769592.1"
                     /translation="DGTLYGATILGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLT
                     HILSEHFENVKTRVQGTVVMFSASGKK"
     gene            complement(271..369)
                     /locus_tag="OO544_004140"
                     /pseudo
     CDS             complement(271..369)
                     /locus_tag="OO544_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885581.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            complement(415..597)
                     /locus_tag="OO544_004141"
     CDS             complement(415..597)
                     /locus_tag="OO544_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR6769593.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNALVCAGYEQIFEDKLSGTRTGRPG
                     LKRALKRLQKGDALVV"
     gene            696..1586
                     /locus_tag="OO544_004142"
                     /pseudo
     CDS             696..1586
                     /locus_tag="OO544_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001533802.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            1589..2122
                     /locus_tag="OO544_004143"
     CDS             1589..2122
                     /locus_tag="OO544_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000972171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR6769594.1"
                     /translation="MQHLKNIVAGNPKTVEQYQLTKNFDVIWLWSEDGKNWHEELKNF
                     QEDTIKLAYTVEGIIIAIDKDVSAINPEGLSVVELPDITANRRADISGNWMFKDGAVI
                     KRTYTEEEQRQQAENEKQSLLQLVRDKTQLWDSQLQLGIISDENKQKLTEWMLYAQKV
                     ESTDTSSLPVTFPEQPE"
     gene            complement(2151..2678)
                     /locus_tag="OO544_004144"
     CDS             complement(2151..2678)
                     /locus_tag="OO544_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001762409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR6769595.1"
                     /translation="MMHLKNIKAGNPKTPEQYQLTKKAGVVWLFCEDGKNWYEEQKNF
                     QPDTIKIAYDENNIIVAVSKDVSTINPEGLSIVEVPDITANRHADNNGKWMFLDGKVV
                     KREYTEQELQQQAESQKAALLSEAESVIQPLERAVRLNMATDEERTRLEAWERYSVLV
                     SRVDTANPEWPQKPE"
     gene            complement(2680..>2966)
                     /locus_tag="OO544_004145"
     CDS             complement(2680..>2966)
                     /locus_tag="OO544_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6769596.1"
                     /translation="ALGSGAQVASSGDIYGSVWENNWLSTWLHNHVVRDIRLGSIEYK
                     NVWRDYGFGDASGYVLTAAINSNADDIVDTVARRPIQKLIGGIWYNVGSV"
BASE COUNT          838 a          661 c          649 g          818 t
ORIGIN      
        1 gatggaactc tatacggagc cacaattctt ggcgatggag ttgtgcacaa tagcttcggt
       61 caaaaactga tgcgcattta caatcagaaa ggcatctttt caaacacaaa agattccgaa
      121 gaaggcttaa cacatatact ctcagagcat ttcgagaatg ttaaaaccag ggttcaaggt
      181 actgtagtaa tgttttccgc ttcagggaaa aaatagcatc caaccgcagc acgttcttgc
      241 ttaagacgtg ctgcggcata atcccaatga ttactccctg acagggttcg taggccactc
      301 aatatcaggt gcagttgatg tatcaacacg gttcagcaac acccgatact ttttctaggc
      361 ttccaccacc agcacgacaa gatgccgcat acagtgcccc agtcagtcca gttttcagac
      421 aaccagtgcg tcaccttttt gaaggcgctt taaagcacgt tttaatccag gtcggcctgt
      481 ccttgttccg cttaatttat cttcaaatat ttgttcatat cctgcacaaa caagagcgtt
      541 tcgttgcagg tctgtattct ggtcatttgt tgataccctt acatagccaa tcagcacgct
      601 gaatctcccg tccaaaagca caaatcatgc catgcaggcc agaaaccgcc attatctaaa
      661 acctcggttt acgagaactc ggcacaagcg gggagaaaat accgttactc agtacagcga
      721 atacctggac taatcgacaa acattcagcg gtggcctttc tggtgaacta tccggcaatg
      781 cttctacagc tgcaaaatta aaaactgcca ggaaaataag caatgtggct tttgatggtt
      841 cctccgatat cacattaaaa gcaagtcatg ttggtgcgtt tgccttaggg aaaacaggaa
      901 gcaccgttgc gaatgataaa gcagttggat ggaactggag tagcggagcc tataacgcaa
      961 ctattagtgg tgcatcaacg ttaattattc atttttatat gggagaagga agttgtcctg
     1021 cagctcagtt tcggattaat tataaaaatg gcggtatttt ttatcgttca gcccgtgatg
     1081 gttatggttt tgaagccgac tggtccgaat tttacaccac cagaaaacct tcagcaggag
     1141 atgttggtgc actgccgtta tctggtggtc aactgaatgg tgcactgggt atcggaacat
     1201 ccagtgctct tggcggtaat tcgattgtat tgggtgataa tgacacgggc tttaaacaaa
     1261 aaggcgatgg taatctggat gtttatgcta ataacgtcca tgttatgcgc tttgtctccg
     1321 gaagcattca aagtaataaa accataaata ttacgggacg tgttaatccc tcggattacg
     1381 gtaactttga ttcccgctat gtgagagatg tcagacttgg cacacgagtt gtttagacca
     1441 tgaagaaagg cgtgatgtat gaaaaatcag gccatgtaat cacggggctt ggtattatcg
     1501 gtgaagtcga tggtgatgat ccggcagtat tcagaccaat acaaaagtta attaacggaa
     1561 catggtataa cgtatcgcag gtataatcat gcagcattta aaaaacattg tcgcaggtaa
     1621 tccaaaaacc gttgaacaat accagctaac aaagaatttt gatgttatct ggttatggtc
     1681 cgaagatgga aaaaattggc atgaggaatt aaaaaacttt caggaggata caataaaact
     1741 ggcttacaca gttgagggaa taattattgc tatagacaag gatgtatcag caattaatcc
     1801 agaaggttta agtgtcgttg agttgcctga tattacagca aatcgccggg ctgatatttc
     1861 gggcaactgg atgttcaaag atggtgcggt gataaagcga acttataccg aggaagagca
     1921 aaggcaacaa gcggaaaatg aaaagcagag cctgctgcag ctcgtcaggg ataaaaccca
     1981 gctgtgggac tctcagctac agctgggcat catttccgat gagaataaac aaaaattaac
     2041 cgagtggatg ctctatgcgc agaaggttga atccacagac acctccagcc tgccagtaac
     2101 gtttcccgaa caaccagaat gagagaaggc ccgctatcgg gccttaattt ttattcaggc
     2161 ttttgtggcc attcaggatt tgccgtatcc acacggctga ccagaacact gtagcgttcc
     2221 catgcttcca gtcgtgtgcg ttcctcgtct gttgccatat tcagcctgac agctcgttcc
     2281 agcggctgga tgactgattc agcttcggaa agcaatgcgg ccttttgtga ttcggcctgt
     2341 tgttgcagtt cctgttctgt atattccctc tttactactt taccatccag aaacatccat
     2401 tttccgttat tgtctgcgtg acgatttgcc gtaatatcag gaacttcaac tatgcttagc
     2461 ccttcaggat taatagttga aacgtcttta ctgacagcaa cgatgatgtt gttctcatcg
     2521 taagcaattt ttatggtgtc tggctgaaaa ttcttttgtt cctcatacca gtttttgcca
     2581 tcttcgcaaa agagccatac cacgcccgct tttttagtta gctggtattg ttccggtgtt
     2641 ttcggattcc cggccttaat atttttcagg tgcatcatct taaacactcc ccacgttgta
     2701 ccatattccc ccaatcaatt tctgaattgg ccttctggca acagtgtcga caatatcatc
     2761 cgcattgctg ttaattgcgg ctgttaaaac ataacctgac gcatcgccaa agccgtagtc
     2821 gcgccatacg tttttatatt caatgctgcc aagacgaata tcccgaacga catgattatg
     2881 cagccatgtg cttaaccagt tgttttccca cactgaacca taaatatcgc cactagacgc
     2941 aacttgcgca ccacttccca gcgcaa
//
LOCUS       DAJTZL010000299         2957 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.302, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000299 DAJTZL010000000
VERSION     DAJTZL010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2957
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.302"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            2..1231
                     /locus_tag="OO544_004146"
     CDS             2..1231
                     /locus_tag="OO544_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HCR6769597.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSACEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
     gene            1691..2323
                     /locus_tag="OO544_004147"
     CDS             1691..2323
                     /locus_tag="OO544_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6769598.1"
                     /translation="MGLCSRYKSLTCNSCSMHCQIMPEESPRLQYCANSCFCMWPEES
                     SYFNRGVVEGILTKNHNARLSGYIFVDFSVSFLRLFLEKDWIDYLASTDMGIVLVSDR
                     NMQSLANYWRKHNTAISAVIYNDDGLDVANEKIRQLFIGRYLSFTRGNTLTQMEFTIM
                     GYMVSGYNPYQIAEVLDMDIRSIYAYKQRIEKRMGGKINELFIRSHSVQH"
     gene            2657..>2957
                     /locus_tag="OO544_004148"
     CDS             2657..>2957
                     /locus_tag="OO544_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR6769599.1"
                     /translation="MYWIINDNIEFWPEHRKLISVHNADLNVVLTTPASRCLSLLLEA
                     FPDVVAQQDFFTRVWEEEGMRVPTNTLYQNISIIRRGFRAVGDTTHSLIATVPRED"
BASE COUNT          863 a          588 c          573 g          933 t
ORIGIN      
        1 aatgtctaat atttggtcta aagaagaaac tctgtggagt ttcgcgctct acggcacagc
       61 cgttggtgca ggcacgctct tccttcctat tcagttaggt tcggcagggg ctgtggtcct
      121 gtttattact gctctggtcg cctggccttt aacatattgg ccacataaag ccttatgcca
      181 gttcatcctc tcatcaaaaa catcagcatg tgaagggata acgggcgcgg taacacacta
      241 ctatggcaag aagattggta atctgattac cacgctgtac ttcatcgcct tttttgtcgt
      301 cgtgttgata tatgcagtgg caattaccaa ctcacttacg gaacagctgg caaagcatat
      361 ggttattgat cttcgcatcc gtatgttggt gagtctgggt gttgtattaa ttctgaatct
      421 catttttctg atgggacgtc atgccactat tcgggtaatg ggatttttgg tattcccatt
      481 gattgcctat ttcttatttc tttccattta ccttgtcggt agttggcaac ctgatctatt
      541 aacgacccag gtagagttca atcagaatac ccttcaccag atatggatat cgattcccgt
      601 gatggttttc gcctttagcc atacgcccat tatttctacg tttgccatag acagacgtga
      661 aaaatatggc gaacacgcta tggataaatg caaaaaaatt atgaaagtcg cttatctcat
      721 catctgcata agtgtactgt tctttgtctt tagctgcctg ctttctattc caccttcgta
      781 tattgaagcg gctaaagaag aaggggtcac cattttatcg gcgctttcta tgctgccgaa
      841 cgccccagca tggttgtcaa tttccgggat tattgtcgca gtagttgcga tgtcgaaatc
      901 attcctgggt acgtactttg gcgttattga aggtgccacg gaggtcgtca aaacaacact
      961 acagcaggtt ggtgtaaaga aaagtcgtgc atttaaccgc gcactatcaa ttatgttggt
     1021 atcgctgatt accttcattg tttgttgcat taacccgaac gcgatttcga tgatttatgc
     1081 gatcagcggc ccgctcattg ccatgatact tttcatcatg cctacgttgt caacgtatct
     1141 gatcccggcg cttaaaccct ggcgctccat cggaaatctg attacgctga tcgtgggtat
     1201 cctgtgcgta tcggtaatgt tctttagcta atatccgttg tggatggttc aaaattgcta
     1261 caattttgaa ccattccatt tttcataata ttcatttgtt attattccgc cctatcactt
     1321 ctctcgtcat atcctagaat tatcagttag ttaaacacac aaagagatct gaacatctta
     1381 aataagacta tttaagatgc ataacttaga ttcgcaagat atcttctggt cattcaaaaa
     1441 caatttgcga tatagatatt ctcataccat gagatagtta tctggaccac gttcgaccat
     1501 gcaagattaa atcagtgaaa cataatcata tttaataacg agaaatgcat ttttaaatgt
     1561 cttaatcaga attatagcaa atacagatta atcgcaattt ttcccagcaa aattacaaaa
     1621 tcaagatgta ttgacatcat caatcaccaa tgaaattatg caattatata cggataggga
     1681 ggttcttaac atggggcttt gtagtcgtta taaaagtctt acatgcaata gttgctcaat
     1741 gcattgccaa ataatgccag aagagtcacc gcgtttgcag tattgcgcaa actcgtgttt
     1801 ttgtatgtgg cccgaagaaa gttcatattt taatcgtggt gtggtggaag gtatcttaac
     1861 aaaaaaccac aatgcgagat taagcggata tatttttgtg gatttttcag taagtttttt
     1921 acgtctattc cttgaaaaag actggattga ctatcttgca agtactgaca tggggatagt
     1981 tttagtcagc gaccgcaata tgcaatcgtt agctaactac tggcgaaaac acaacaccgc
     2041 aatatctgct gtcatatata atgatgatgg tcttgatgtt gccaatgaaa agatcagaca
     2101 actatttatt ggtcgttatt tatcatttac cagaggtaac acattaactc agatggaatt
     2161 caccataatg gggtatatgg tttctggtta taatccatat cagattgctg aagtgctgga
     2221 tatggatatc cgtagcatct acgcgtacaa gcaacgaatc gaaaagagaa tgggtggtaa
     2281 aataaacgaa ttatttattc gttcacattc ggtccaacat tgatactcaa cgaccagcca
     2341 gaatcatact ctgttcatac gggcatctat tgaaatgccc gtattttata aataagcagt
     2401 aaccccaaga gtaaacaaca tagtatttaa aaccatcaca cgaaaaattc agaagcatta
     2461 cgaaatatga atttacaaac aatagtggca taaatgttaa ccatgttaat ttacgtaaag
     2521 ttttacgttg caacattaaa gcctcatttc aatcatcatg ataaatataa aattaatata
     2581 tatttatgcc gtaaatacca atatacttag caaactatgt gatctccatt tcgattgatt
     2641 tagtgtttat tgacgtatgt actggattat taacgataat atcgagttct ggcctgagca
     2701 ccgaaaatta atatcggtac ataacgccga tcttaacgtc gttctgacaa cgccagccag
     2761 tcgatgttta tcacttctac ttgaagcttt tcctgatgtg gttgcacaac aagatttttt
     2821 caccagagtc tgggaagaag aaggtatgcg tgtgcctact aacacgttat atcagaacat
     2881 atccattatc agacgcggat ttcgcgctgt tggtgatact acccactcgc taattgcaac
     2941 cgtgccgaga gaggatt
//
LOCUS       DAJTZL010000300         2936 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.303, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000300 DAJTZL010000000
VERSION     DAJTZL010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2936
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.303"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            35..262
                     /locus_tag="OO544_004149"
     CDS             35..262
                     /locus_tag="OO544_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HCR6769600.1"
                     /translation="MRLIVVSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDTHHGPYE"
     gene            complement(263..637)
                     /locus_tag="OO544_004150"
     CDS             complement(263..637)
                     /locus_tag="OO544_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secY/secA suppressor protein"
                     /protein_id="HCR6769601.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAEDANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWDEGE
                     FQLEPPLDTEEGHAAADEWDER"
     gene            complement(720..1766)
                     /gene="mdtG"
                     /locus_tag="OO544_004151"
                     /pseudo
     CDS             complement(720..1766)
                     /gene="mdtG"
                     /locus_tag="OO544_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
     gene            complement(2097..2672)
                     /locus_tag="OO544_004152"
     CDS             complement(2097..2672)
                     /locus_tag="OO544_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR6769602.1"
                     /translation="MSRTFAYCRVSVETSTTENQIQAIKKAGYDIPKSRVIEEVVSGS
                     VSAMDRDGFANLVNHKLEEGDTLVVLKLDRLGRDNIDLQNTVTLLNDKGVKLICLDLP
                     VKDLSSSEGKLVLQLFSTFAEFERNRIRERTKQGLERARVQGKVLGRPIASDTTRAVL
                     EYKERGLSQSQVAKVLNLSVRTVSRHWTKTL"
BASE COUNT          761 a          695 c          687 g          793 t
ORIGIN      
        1 ttatgccggt atttttttac ggaagaggta tcaaatgcgt ttaattgtgg tgagcatcat
       61 ggtgaccttg ctgagtggct gtggcagcat cattagccgc actataccgg ggcaggggca
      121 cggcaaccaa tattatcccg gtgtgcaatg ggatgtgcgt gactccgctt ggcgttatgt
      181 cacaatcctt gatctgccat tctccctggt ttttgatact ttactgctgc cgatcgacac
      241 tcatcatggc ccgtatgagt gattaacgtt catcccactc atcagctgca gcgtgtcctt
      301 cctcggtatc cagcggtggc tcaagctgaa attccccctc gtcccattca tgtaatgtat
      361 tctcttcctg ccactcctgg cggatctcta tctcatcata gtcgccatca aaaacacttt
      421 gcgcggcttc accgctaagc ataggtaaac attcaccttc ttccccttcg tcggcaaaaa
      481 actcaacttg ccacatgatg tcgccgtcct gcaaaacgta tttttgggca ttgaactgtt
      541 gcacattcgc atcttcggcg tcgatgccgg ggttgtctgc aagaaattct tcgcgtgcag
      601 cgtcaatggc ttcttcaagc gttgcgtaca tggtcatcac gattctccct ttgagttgat
      661 gaggtttcag ggaaaaggat agctgattct ccgcttttgc aagtatgaaa ggcgaaaaat
      721 cagttcgata cctggggtat tcgacgacga cgtagactgt tccatgaata gactgcgttg
      781 aataacacta cgccagcggt gacgagaaat accgctctga aaccgtagtt cgctgaaatc
      841 gctgctccca tcaatggtcc ggtaacgttg ccaatatcac gaaacgattg gttatagctg
      901 aagatacgtc cggcgatttg gttgctcgag ttataaacta acagtgtctg tacggcgggg
      961 agtagtgcac catcggcggc accgagcaaa aaacgtaaaa tcccaagttg caatggcgtc
     1021 tgaacgtaag acattgggat caacagcagt acagaaaaga tcagcgccgt aatcaggatc
     1081 ttttcgggtc cgattcgatc gccaagtttg ccgagtcgtg gtgcacttag cagagccgcc
     1141 acgcctggca ccgaggcgat catgccactg ataaaggcga cgttactgac gttacccgcc
     1201 agttcgcgga catacagcgt cagaatgggg gcaattgagc ccgtcgccac ctggatgatt
     1261 aacgtagtga caaacaggct gagtaccagt ttcgggtttt taagtgatgt caccacttcc
     1321 cgcatgtgca gcatctcttt tttgctgatc ggctggaatt tttctcttat gcaaaacagg
     1381 gtgacgaaaa agcagagtat gagcacactg gcggtaataa agaataccgg acgtaagccg
     1441 tagctatcgg cgagcaggcc gccagccatt gggccgagca acgcaccact aacgccgcct
     1501 gtggagagcg tacccagcgc ccagccgctt ttattacgcg gtacttgtgt ggcgataaga
     1561 gcattagcgt tggggacaaa tccgccaagt aacccaagaa gcgcccgcag gatcaaaaac
     1621 tgccagatat tttgtgccag ccccatcaac accatcacga tgcccatgcc gagggcagag
     1681 cgtaatagca tgagttttcg gcctttacgg tcggcgagtc caccccaaaa cggtgaggcg
     1741 atggccgaaa ataaaaatgt aatgctcata tatgctatct taatgcacta cctgataagt
     1801 gagcctatac aggttattgc tgtatgttta tacagtatta ttggtagaag actgtgtaga
     1861 tttggtatag agatgaatag atactattaa ggtaacttat tgaaatcaat aaagattctt
     1921 gtgcagtctg aatagataac ttagcgttat gtctagtcgt ttccctctcc attcctaaaa
     1981 agtgctgtat actggcctat acaacgctat aaagacctct acatagcgcc ttgatagcta
     2041 tgtcacaggt acaaaaaagc cccctataaa ggaggctgtg tagtctatta agggtcttac
     2101 agggtcttgg tccagtgtct gctaacagtc cttacagaga ggttaagaac cttagctacc
     2161 tgagactgag atagtcccct ttccttgtac tctaagaccg ctctagttgt gtcactcgct
     2221 ataggtcttc caagtacctt accttgaact ctagcacgct ctagaccctg tttagtgcgc
     2281 tctctgatac ggtttctttc aaactctgca aaggtactaa atagctgtag aaccaactta
     2341 ccctctgagc ttgaaaggtc tttaacgggt aaatctaaac agatgagctt cacaccctta
     2401 tcattaagaa gggtcactgt attctgtagg tcgatgttat cccttcccag acggtcaagt
     2461 ttaagaacaa caagggtgtc accttcttct agcttatggt tgaccagatt cgcaaatccg
     2521 tccctatcca tagctgacac tgaaccagag acaacctcct ctataactcg tgacttagga
     2581 atatcatagc cagccttctt aatggcttga atctggttct ctgtggtgga agtctctact
     2641 gatacacggc agtaagcaaa ggttcgtgac atagatatcc ctctgggtag tcattaaacg
     2701 gttagtttgt cttgtctata gacattaaag caaatggtag atttgttgtc tatagctata
     2761 gtgactcttc tatgtagaca ttaaacggac gtttcttgtc gtgggtcaca tagatacttt
     2821 tagggcttct gtcaagtgat attcgtagat tttgcaaggg ggaggtgtaa ggctgaaaca
     2881 gggctactaa gaacacatac atctaaggct ggaaagtacc tgagcagata ttcttc
//
LOCUS       DAJTZL010000301         2919 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.304, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000301 DAJTZL010000000
VERSION     DAJTZL010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2919
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.304"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(94..1737)
                     /gene="yeeO"
                     /locus_tag="OO544_004153"
     CDS             complement(94..1737)
                     /gene="yeeO"
                     /locus_tag="OO544_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HCR6769603.1"
                     /translation="MLRHILTAKNLLSNPIFKFPNCLPFLSTVCCICRQFVGENLCSF
                     ADSPSLFEMWFHFLQLRSALNISSALRQVVHGTRWHAKRKSYKVLFWREITPLAVPIF
                     MENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVVVAFSLG
                     KRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKALALTYLEL
                     TVLSYPAAAITLIGSGALRGAGNTKIPLLINGSLNILNIIISGILIYGLFSWPGLGFV
                     GAGLGLTISRYIGAVAILWVLAIGFNPALRISLKSYFKPLNFSIIWEVMGIGIPASVE
                     SVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLPGSALGSASTIITGRRLGVG
                     QIAQAEIQLRHVFWLSTLGLTAIAWLTAPFAGVMASFYTQDPQVKHVVVILIWLNALF
                     MPIWSASWVLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVLGIMLGWGVVGVWMGMF
                     ADWAVRAVLFYWRMVTGRWLWKYPRSEPQKCEKKPVVSE"
     gene            1742..1817
                     /locus_tag="OO544_004154"
     tRNA            1742..1817
                     /locus_tag="OO544_004154"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1775..1777,aa:Asn,seq:gtt)
     gene            complement(1855..2805)
                     /gene="cbl"
                     /locus_tag="OO544_004155"
     CDS             complement(1855..2805)
                     /gene="cbl"
                     /locus_tag="OO544_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HCR6769604.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGTPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFRWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRIDDAFARKGLLA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
BASE COUNT          855 a          781 c          615 g          668 t
ORIGIN      
        1 gaacgaggcg aatattagcg atgcccaccc acaatgtcaa agcctgtttt ttaaatttga
       61 aatcgtttgc tgaaataatc tgcattttgt cgtttattcc gacacaactg gctttttttc
      121 acacttttgc ggttcggatc gagggtattt ccacagccaa cgtccagtaa ccattcgcca
      181 gtaaaacagc acggcccgca cagcccagtc ggcaaacatt cccatccaga caccaaccac
      241 accccagcca agcatgatgc ccagcacata accgacgaca acccggcaac cccacatgct
      301 caacatagaa acccacatgg cgtaacgggc atcgcgagca cctttaaatc cagcgggtag
      361 cacccatgag gcggaccaaa taggcataaa taaagcattt agccaaatca gaatcacaac
      421 gacatgttta acctgtggat cctgggtgta aaacgatgcc ataaccccgg caaagggagc
      481 cgttagccag gcgatggccg ttaatccaag agtggaaagc cagaacacat gccgcaactg
      541 aatctctgct tgcgctatct gccctacccc caaccttcgg cctgtaatga tcgtagaagc
      601 agagccgagc gcacttccgg gtaagttgat aagagccgca attgaaaacg cgataaaatt
      661 tccggcaata acactggtcc ccatcccggc aacgaacatt tgggttaata accgaccact
      721 ggtaaataac actgattcga cactcgcggg aataccaatc cccatgactt cccagataat
      781 gctaaaattc agcggtttaa aatagctctt taacgaaatc cttagcgcag gattaaaacc
      841 aatcgccagc acccacaaaa ttgcaactgc gccaatataa cgagaaatgg ttaaacccag
      901 ccctgccccg acaaatccca gtcccggcca ggagaaaagg ccgtaaatca atatgccgct
      961 aataataata ttaagaatat tcaggctacc gttaatcaat agcggtattt tcgtattccc
     1021 tgcaccacga agtgccccgc taccaataag agtgatggca gctgctggat aactgagtac
     1081 cgtcagctcc agataagtca acgccagtgc tttaacttct gtcgtggcat cacccgcgac
     1141 gaaatcaata atttgttcgc caaaatgatg aataagcgtt gccaacagta cggcaaacaa
     1201 cgtcatgatc accaatgact gccgcgtcgc caccctcgct cgtcgtcgat cccgcttacc
     1261 gagactaaat gccacaacga cagtagtacc aagatcgata gcagcaaaaa aagccataat
     1321 gaccatattg aagctgtccg ccaatcccac gccggccatc gcatcttttc ccagccagct
     1381 gaccagaaaa gtgctcagaa cccccatcaa caggacacag gcattctcca tgaagatagg
     1441 aacagcaagc ggggttatct cgcgccagaa caacactttg tagctcttgc gtttagcgtg
     1501 ccagcgagtg ccgtgaacaa cctggcgtaa agcagaggag atattcaaag ccgaccttaa
     1561 ttgcagaaag tgaaaccaca tttcaaataa tgagggagaa tcagcaaagc tgcaaagatt
     1621 ttcgccaaca aattgtctgc aaatgcaaca aactgttgat agaaacggca aacagttggg
     1681 gaatttaaaa atcgggtttg acaaaagatt tttcgccgtt aagatgtgcc tcaacagcga
     1741 ttcctctgta gttcagtcgg tagaacggcg gactgttaat ccgtatgtca ctggttcgag
     1801 tccagtcaga ggagccaaat ttctgaaaag cccgctttta tagcgggctt tttgctatat
     1861 ctgataatca atttcctctt cactgttttc catcacctgc cgcttgatat cctcaactga
     1921 cagtcctgca ttacaaagtt ccagaaagcg ccagacatag ttacgctgaa gttgtcctcg
     1981 cttcagtccc aaccagacag tattagcatc gaaaagatgc cgcgtatcca gacggattaa
     2041 attctcttcc tcttgttcgc cgctggattg ttcggcaact aatccgatcc caagcccaag
     2101 agcaacatag gttttaatga catcagaatc ctgcgcactt aatacaatat ctgccagcaa
     2161 acctttgcgg gcaaatgcgt catcaatacg tgagcgcccc gtaatcccct gtcggtaagt
     2221 gattaacggc cacttcgcta ttgattccag cgtcaatggt gtaatttgcg ttaagggatg
     2281 atcgagtgga acaagcaaac tatggtgcca acgaaaccac gggaaggcga cgagctgcgg
     2341 gtcattactc aaacgctcgc tggcgatacc aatatcagct tcgccatttt gcaacaacgt
     2401 cgcaatttcc tgtggcgtcc cctggattag ctcgagccga acctccggga aaagttcgcg
     2461 aaaagcttta atgacctctg gcaagctata acgtgcctga gtatgcgtcg ttgcaatagt
     2521 gagaacgcca gacgtatcgt tggtaaacag gtctgcaagc cgacgaacat tactggcttc
     2581 attcagaata cgttctgcaa tgaccagtaa tgctttgccc ggttcagtca tgcccagcag
     2641 tcgcttacct cgtcgaacaa atatttcgat gccaagttca tcctccagtt cccgaatatg
     2701 acggctgacg ccggactgtg aggtaaaaag catattcgca acctctgtca ggttgtaatc
     2761 ctgacgtgca gcctcgcgga ttatctttag ttgttggaaa ttcacggtaa actccgggca
     2821 gttcagattt cccgttattg ttaaagtcta atgcccggca taacaaataa taaaaacccg
     2881 catcttattc catcccgata taacgcttag ctcaccaat
//
LOCUS       DAJTZL010000302         2893 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.305, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000302 DAJTZL010000000
VERSION     DAJTZL010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2893
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.305"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..493
                     /locus_tag="OO544_004156"
     CDS             <1..493
                     /locus_tag="OO544_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000785589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HCR6769605.1"
                     /translation="ADPDYVIWAKEVQGINRAWTFRHWKGTGTVGVMVATNDPERPAP
                     DESVINAVRDHILPLAPVAGSGLYVFGATEKVIPMTIALSKDTPQIRTAIKAELNALM
                     LRDGVPEGRMYLSRISEAISLSAGEVAHRLIVPSSDIDLGETELPVLGEITWQAYDPA
                     RSK"
     gene            493..1083
                     /locus_tag="OO544_004157"
     CDS             493..1083
                     /locus_tag="OO544_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2313 domain-containing protein"
                     /protein_id="HCR6769606.1"
                     /translation="MDSLQDDYTKLLYGLMPPGPAWSDTDGVLDGLAPSLVRVHQRAD
                     ELVIEIDPGQSTELIERYEELYGLPDSCSPVGTQTLRQRQQRLEAKANVAGGINEQFF
                     LDQLEALGYTGVTIEQFQHLDASPDPEWGDRWRYFWRVTLPMDAGAQWQTCTDACNTP
                     IRTWGDTVAECVINKLCPSHTVVLFSYPDEDEDAQD"
     gene            1070..2110
                     /locus_tag="OO544_004158"
     CDS             1070..2110
                     /locus_tag="OO544_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6769607.1"
                     /translation="MHRIDTLTAVKDKFGPGKNGFTDGNLRTGRLATWLNSAMWNAIQ
                     EEICGVIEKAGIELNKEEHDQLYKAILLLVGGAINEEALLIKNNLSDVEDRDEAVENL
                     GLKPTVDKAKNAVQRDGDTMTGELKIRGVNALRIFNEAFGLIFRRSEECLHLIPTSEG
                     QGENGDIGPLRPFTINLRTGEISMSHKVSVGGGSQVNGALGIGVQNALGGNSIVLGDN
                     DTGFKQNGDGLLDVYANSVHVLRFQSGSIQSNKAVNVTGRVTPSDYGNFDARYQQRNG
                     GVQDVRYGYEMYYPPGSNTVSWTFRSPSGHGLSGISISDTGRNSADNVNGVYYRPLQK
                     LINGTWYNVASI"
     gene            2113..2658
                     /locus_tag="OO544_004159"
                     /pseudo
     CDS             2113..2658
                     /locus_tag="OO544_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902876.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            2682..>2893
                     /locus_tag="OO544_004160"
     CDS             2682..>2893
                     /locus_tag="OO544_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCR6769608.1"
                     /translation="MSVVISGALTDGAGIPMSGCQIILKSRVNTSEVVMRTRADVVTG
                     NNGEYSFEAQVGKYCVYLKRDWRDEY"
BASE COUNT          752 a          633 c          824 g          684 t
ORIGIN      
        1 cgcagaccct gattatgtta tctgggcgaa agaagttcag ggtattaacc gtgcatggac
       61 tttcagacac tggaaaggca ctggaacggt tggcgtgatg gtggcgacaa acgatccgga
      121 acgcccggcc ccggatgaaa gcgtgattaa cgcagtcagg gaccacatcc ttcctctggc
      181 acctgttgcc ggaagcggat tgtatgtatt cggtgccaca gaaaaagtca tcccgatgac
      241 gattgcatta tcgaaagaca caccgcagat caggactgca ataaaagcag agctgaatgc
      301 actgatgtta cgggatggtg tgccagaggg gcgtatgtat ctgtccagaa tcagtgaggc
      361 catcagtttg tctgctggtg aagtggccca ccggttaatc gtcccttcat cggatattga
      421 cctgggggag accgagcttc cggtgctcgg cgagatcacc tggcaggctt atgacccggc
      481 aaggagtaaa taatggattc gttacaggat gattatacaa agctgctgta cggactgatg
      541 ccgccggggc ctgcatggtc agataccgac ggtgtacttg acggtctggc accatcgctt
      601 gtgcgtgttc accagcgggc tgatgaactg gtgattgaaa tcgatcccgg tcagtccaca
      661 gagctgattg aacgttatga agaattgtat ggtttacctg attcctgttc ccctgtcgga
      721 acccagacat tacgccagcg tcagcaacgt cttgaagcaa aagccaatgt ggctggtggc
      781 ataaatgagc agttcttcct ggatcagctt gaggcgctgg gatataccgg cgtaacgatc
      841 gaacagttcc agcacttgga tgcaagcccc gatccggaat ggggagatcg ctggcgttat
      901 ttctggcgtg tgacgttgcc gatggatgcc ggtgctcagt ggcagacatg cacggacgcc
      961 tgcaacacac cgatccggac gtggggggat acggttgcgg agtgcgtgat taataaatta
     1021 tgtccgtcac acaccgtcgt tttattttcc tatccagatg aggatgaaga tgcacaggat
     1081 tgatacgctg actgcggtaa aagataagtt tggtccgggt aagaacggat ttactgacgg
     1141 aaatcttcgc acaggacgtc ttgctacctg gctgaacagt gccatgtgga atgccattca
     1201 ggaggaaatc tgtggtgtca ttgagaaggc cgggatagaa ctgaataaag aagaacacga
     1261 ccagctatat aaggctatat tattgctggt gggcggtgca attaacgaag aggcattgct
     1321 gataaaaaat aacctttcgg atgtggaaga cagggatgag gctgttgaaa acctcggatt
     1381 aaaacccacg gtggataagg caaaaaatgc cgttcagcgt gatggcgaca ccatgaccgg
     1441 agagctgaaa atccgtggtg ttaatgcgct gaggattttc aacgaagctt ttggcctgat
     1501 ttttcgtcgt tcggaagagt gcctgcacct tattcccacc agtgaaggtc agggggaaaa
     1561 tggcgatatt ggtcccctgc gcccgttcac cattaatctg cggacgggtg aaatatccat
     1621 gtcgcataaa gtgtctgttg gcggtggttc gcaggtcaat ggtgcgctgg gtatcggcgt
     1681 tcagaacgcc ctggggggga attcaattgt tcttggtgat aatgacaccg gattcaaaca
     1741 gaatggtgat ggcctgctgg atgtttatgc caatagcgtg catgtgttgc gttttcagag
     1801 tggcagtatc cagagtaata aagctgtaaa cgttacagga cgggtaacac cgtcagacta
     1861 cggaaacttt gatgcccgtt accagcagcg aaatggtggc gtgcaggatg tgcggtatgg
     1921 ttatgaaatg tattaccccc ccggcagtaa caccgtttcg tggacatttc gctcaccttc
     1981 gggacacggg ctgtcaggga tatcgatatc ggataccggc cgtaactcag cggataacgt
     2041 caacggcgtg tattaccgac cgctgcaaaa actgattaat ggcacctggt ataacgtagc
     2101 gagtatttaa caatgttgca tttaaaaaat attacggcag gtaatccgaa aaccgcggaa
     2161 taatatcagc tgacaaaaca gtatgatgtc acctggcttt tttcggaaga cggcaaaaac
     2221 tggtatgaag aacagaagaa ctttgccagt gacaccataa aaatggttta caccggagac
     2281 gggcgcgtgg tgtgggtcgg taaggatgtg acaggcattg aaccccgtaa cgccagtgtt
     2341 attgaagttc ctgatattac cgccaaccgc cgcattaccg cgccgggtta ctggttttac
     2401 cgcaatgatg aatttgtctt tgactacaga ctcaaagcgg aagatgagcg tgatgccctt
     2461 ctggctcagg tcagtgcccg gacaggggaa tgggaagaag acctgctgct ggggctaatc
     2521 agcgacgaag accgggaaaa actgaaagcg taccgtattt acgcgaaatc gctgcaggcg
     2581 atggatttca gcaccatcac tgataaatcc tcatataacg ccattgaatg gtccgtctct
     2641 ccggaaggtt cttcctgatt taatttatcg cgagaaaaac tatgtctgta gtgatatcag
     2701 gtgcgctgac tgatggcgca ggcatcccca tgtccggatg ccagattatt ctgaaatccc
     2761 gtgtaaacac ctcagaagtg gtgatgcgta ccagggctga tgtagtgacc ggaaataacg
     2821 gtgaatattc gtttgaggca caggtcggaa aatattgcgt gtatctgaaa cgggactggc
     2881 gcgacgagta ctg
//
LOCUS       DAJTZL010000303         2890 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.306, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000303 DAJTZL010000000
VERSION     DAJTZL010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2890
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.306"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            122..445
                     /gene="soxS"
                     /locus_tag="OO544_004161"
     CDS             122..445
                     /gene="soxS"
                     /locus_tag="OO544_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HCR6769609.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(448..2034)
                     /gene="pdeC"
                     /locus_tag="OO544_004162"
     CDS             complement(448..2034)
                     /gene="pdeC"
                     /locus_tag="OO544_004162"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HCR6769610.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIAPVNGYTIAPADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSKEASAATFSPLIHLKDLTVQRNGYLYATVY
                     STKRPIAAIVATSYQRLITHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQ
                     RALEKHQLCLYYQPIIDIKTEKCIGAEALLRWLGEQGQIMNPAEFIPLAEKEGMIEQI
                     TDYVIDNVFRDLGAYLATHADRYVSINLSASDFHTSRLIARINQKTEQYAVRPQQIKF
                     EVTEHAFLDVEKMTPIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVE
                     TLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGWFFAKAMP
                     PQVFMQWMEQLPARELTRGQ"
     gene            2464..2744
                     /locus_tag="OO544_004163"
                     /pseudo
     CDS             2464..2744
                     /locus_tag="OO544_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
BASE COUNT          801 a          675 c          632 g          782 t
ORIGIN      
        1 tagcagcgct ttaatgcggg gtaatttctt ttccataaat cgctttacct caagttaact
       61 tgaggaatta tactccccaa cagatgaatt aacgaactga acactgaaaa gaggcagatt
      121 tatgtcccat cagaaaatta ttcaggatct tatcgcatgg attgacgagc atattgacca
      181 gccgcttaac attgatgtag tcgcaaaaaa atcaggctat tcaaagtggt acttgcaacg
      241 aatgttccgc acggtgacgc atcagacgct tggcgattac attcgccaac gtcgcctgtt
      301 actggccgcc gttgagttgc gcaccaccga gcgtccgatt tttgatatcg caatggacct
      361 gggttatgtc tcgcagcaga ccttctcccg cgttttccgt cggcagtttg atcgcactcc
      421 cagcgattat cgccaccgcc tgtagtttta ttgcccgcgc gttaactccc gcgcgggtaa
      481 ttgctccatc cattgcataa acacctgcgg cggcatcgcc ttcgcaaaaa accatccctg
      541 gcaatagcgc acgccgcgtt tgcgcagcca gttaacctgc tcctcagttt cgacgccttc
      601 agcgatcgtt tttaacccca ggctgtgcgc cagctcgatg atgtgttccg caatcaaatg
      661 actggttttg tgggtggtca gcgtttcaac aaacgattta tcgattttca aaatatcgac
      721 attcaatgat ttaaggttat gcaagttaga gtagccaata ccaaaatcat caattgccac
      781 ttcgtaacca gcctggcgga aagccagaat aatcggcgtc attttttcga catcaagaaa
      841 tgcatgctca gtcacttcaa atttaatttg ctgcggacgc accgcgtatt gctctgtttt
      901 ctgattgatt cgcgctatca accgtgacgt atgaaaatcg gaggccgaca ggttaataga
      961 aacatagcga tcggcatgtg ttgccaagta cgcgcccaga tcgcggaaga cattatcaat
     1021 aacataatca gttatctgtt ctatcatccc ctccttttct gccagcggaa taaactctgc
     1081 cggattcatt atttgcccct gctcaccaag ccaacgtaac aacgcttcag cgccgataca
     1141 tttttctgtt ttgatatcga ttattggctg gtaataaaga caaagttgat gtttttcgag
     1201 ggcgcgttgc aatttacgtt tgggagataa atagttttgt cgaatacgta gccagagtaa
     1261 tagcagaaca agactcccca aaataccggc gggcaacgca aaaataagat gattataaaa
     1321 atgggttata agacgttgat atgaagtcgc aacaatggct gcaattgggc gttttgtcga
     1381 ataaactgtc gcatataaat agccatttct ttgtacggtt aaatccttca aatgaatcag
     1441 cggagaaaac gttgctgccg aggcctcttt gcttaacgag aaaaaggttt tcgtcaccgt
     1501 atcatacaca ccccattgca atgtcgggtc atcagacatc acttcactcc agaagagagg
     1561 gttgataacc gccacataat ttccccgctg catataggtc attttatagc cagagaaaaa
     1621 aggcgtatcg cggtaataat agatagaaac gttaggttca cgcttataat cggccggtgc
     1681 aatcgtatag ccgtttacag gcgctatcag cgatgagcat aaaaaatggt tatcacgggc
     1741 atagatcaat tcattaatat aaagatagcc acgaataata ttcaacattc gcttttgatg
     1801 ggctggagtg caaacttgcc cctgatagcg ttcagctgca tcgctcacta aatctgcctg
     1861 gcgaatgacc agctcggatt tatccagtgc cagttgagca aaggttcgga gctgattatt
     1921 cacttctgat tttgcccaaa ggaaggcaat ccatagcgaa agaatgatgg gaaagagaac
     1981 taaaaagata atgcccggca acgccagtaa ttggtgtcgt gcacgatgac tcattgctat
     2041 caatttcctg cctgtttata cccggccatt gcaaggtgaa aaccctgggt accgacgtct
     2101 ttagataaat cttacggtat aaatagcact tttgcctgat ttgaaattga tacctttcta
     2161 atcgtcacct ggatgaaaat aattcattga tattcaaaat taatttccta caggaatgca
     2221 gaggcggcgg gaagatatgc ccttaatgcc actgaaaaac cacataatct gtacagcaat
     2281 aaaagtcacg gcctaatatg gctaacaaac aatcaattgt gatatagctc acaaaattaa
     2341 tgaaacaaac agagtgtttc atttttgtgt tctgcaagcc ccgcgatagc cagagtgtac
     2401 cacgcctccc gtgaacaacg ccgcgctgtc cagggcatcg gctcttttca gaggaattgt
     2461 ttgatggcta ccctcaccac tggcgtggtt cttcttcgct ggcagcttct tagtgccgta
     2521 atgatgtttc tggccagcac atcaacatcc gttttcgtcg gtctgattat gtcgggcttg
     2581 cggtgatcag cagcggtctg ggcgttgttt ccgcctgttg gttcgcaatg gggttgcttg
     2641 gcatcacaat ggcggacatc accgctatct ggcacaacat cgagtcggtg atgatagaag
     2701 agatgaatca gacaccgcca caatggccaa tgattttgac ctgatacgta gaagcctcca
     2761 taacggaggc ttctttttta tggctggcag atgctttaat catccacctt aaaacaatat
     2821 aacctattgt tttaatgaca aatcagaacg gaatgtcatc atcaaagtcc atcggcggct
     2881 cgttagacgg
//
LOCUS       DAJTZL010000304         2878 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.307, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000304 DAJTZL010000000
VERSION     DAJTZL010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2878
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.307"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..954)
                     /gene="curA"
                     /locus_tag="OO544_004164"
     CDS             complement(<1..954)
                     /gene="curA"
                     /locus_tag="OO544_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HCR6769611.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPSDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHAIEVLGFDVCLDHHADDFAEQLVKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYHEDITDGL"
     gene            1135..1653
                     /gene="mddA"
                     /locus_tag="OO544_004165"
     CDS             1135..1653
                     /gene="mddA"
                     /locus_tag="OO544_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HCR6769612.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHHSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            1650..2099
                     /locus_tag="OO544_004166"
     CDS             1650..2099
                     /locus_tag="OO544_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR6769613.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLMLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(2100..2333)
                     /locus_tag="OO544_004167"
     CDS             complement(2100..2333)
                     /locus_tag="OO544_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcY family protein"
                     /protein_id="HCR6769614.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(2419..2592)
                     /gene="ortT"
                     /locus_tag="OO544_004168"
     CDS             complement(2419..2592)
                     /gene="ortT"
                     /locus_tag="OO544_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HCR6769615.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            2787..>2878
                     /gene="yncL"
                     /locus_tag="OO544_004169"
     CDS             2787..>2878
                     /gene="yncL"
                     /locus_tag="OO544_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HCR6769616.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWF"
BASE COUNT          691 a          738 c          692 g          757 t
ORIGIN      
        1 taaaccatca gtaatatctt cgtggtagtg gattttatcc tctttcaccc attgccccat
       61 ctccttctga aactcatgga tgcggtgacc ataatcctga gcgataataa acccttgcaa
      121 gcgaatacgt tttttcagca ccgttgccat caacaggggt aaacgatccg gaccgggtgg
      181 tagctctgta gcgttatagc tgctcactaa gccacaaacg ggaatgcgcg cagatgtatt
      241 aagtaatggt agcactgcat caaatacctt accgcccacg ttttcataat agatatcaat
      301 accttttggg cacgctttca ccagttgttc ggcaaaatca tccgcgtggt gatcaagaca
      361 aacatcgaag cccagcacct cgatagcatg gcggcatttt tccgcgccac cggcaacccc
      421 caccaccctg caacctttaa gtttgccgat ttgccccacc gttgctccca caggacctgt
      481 cgccgcagct accaccaacg tttcgccctc tttaggctga ccgatatcca gcagccccat
      541 ataagcggta aagcctggca tccccagcac acccagcgac cacgatggat tttgcggatg
      601 atcgccaagt ttcaccagat catcaccact ggatatgtca tagtcctgcc atccactgta
      661 gcccagcacc cagtcgctag gctgataatc aggatgattc gactccacaa cacggctcac
      721 cgtgccgccg accatcacgc cgccaatatc aacaggcggt gaataagatg gctcatcgct
      781 catacgtcca cgcatatacg ggtccaggga caaataaact gtgcgcagta acacctgtcc
      841 ttcacccggt gtggcgacat catcttcttc aagacggaaa ttctccggaa ccggtgcgcc
      901 atgtggacgc gaggccagaa cccaacgtcg attacgctgc ttttgttgcc ccataatgct
      961 ctcctgtgct tttggcatga atccatcaat agtagcaggc tcacgggatt ggttttgttt
     1021 gcagccgtca catcccctct gcaacacgca ataatttcat cgaggaatcc gttgcttcgg
     1081 gctgttttcg atagtattga taacatcatc atcattattt tatcgaggcc agacatgtcc
     1141 atccgttttg cccgcaaagc cgattgtgct gccattgcgg aaatttataa ccacgccgtg
     1201 ttgtatacgg cggctatctg gaatgaccaa acggttgatg cagataaccg cattgcctgg
     1261 tttgaagcgc ggaccatagc aggttatccg gtgctggtaa gtgaagaaga tggcgtagtg
     1321 acgggatatg cctcgtttgg cgactggcgt agtttcgatg gttttcgcca taccgtggaa
     1381 cattcggttt atgtccatcc cgatcatcag ggcaaaggtc tggggcgtaa attgttaagc
     1441 cgattgattg atgaagcgcg ggattgcggg aagcatgtca tggtcgccgg gatcgaatcg
     1501 caaaatcagg cctcgctgca tctgcaccat tcgctgggat ttgtcgtcac cgcacaaatg
     1561 ccgcaggtgg gcactaaatt tgggcgctgg ctggatctga catttatgca gttgcaactc
     1621 gacgaacgca ctgaaccgga cgcgattgga tgaatcagtc gctcaccctt gcttttttga
     1681 ttgccgccgg aattggtctg gtagtccaga acacgctgat ggtgcgcatc acccagacct
     1741 cctctaccat tctcatcgcc atgttgctga actcactggt ggggattgtg ctctttgttt
     1801 ctattttatg gtttaaacaa ggcatggcgg ggtttggcga actggtgtcc agcgtgcgct
     1861 ggtggacact tatacccggc ttgctgggat cgttttttgt cttcgccagt atcagcggat
     1921 atcagaatgt gggagcggcg acaactattg cagtactggt cgccagccag ctaattggcg
     1981 gtctgatgct ggatatcttc cgcagtcatg gcgtaccact acgggcgttg ttcggcccaa
     2041 tttgtggcgc aattttactt gtggttggcg cctggttggt agccagacgc tcattttaat
     2101 cagaggatgg tgccgccttt ggttaactgt tcgtggcgcg cttccatatc ttctttatgc
     2161 ttgcgaccgt gatgagcaat agcggtgcgc aaacgctgct gctgggtgta gcgatcttcc
     2221 cgacttaact ccgcgtcatc gctcagggcg attaataatt cgttcatatg ggcaatgacg
     2281 ctctcttcaa tcgcggcatc cacgcgcgcg atgacttcat ccagatgcga catactttct
     2341 ccttaaccac ttgtgaaatt tatcgattca tccaaatgat ataagtctgc gtggtgccgt
     2401 caagccagtg agtaagattt aaaacagtga aaacaacagc gccaccggga aactcatcgg
     2461 ccatgttgcc cccaccagaa aggcgcttaa gaaacggatg cgtttcttat cgtgagaaag
     2521 aaaccaggtg ataagaaatg cgattgctgc cataaccgca taaaaaacaa gcatatgttg
     2581 atagagagac atgtacgggc gtccagccag atgaataaaa cgcgcgcaaa tatgcgtcat
     2641 tttttgacgt gcatcaagtt ttttcaccag tttgagaaaa aatcatcaaa cagcaacttg
     2701 cccttatcca tcttcagaat gagtattatt gcggattttc ttaacccgta ctatacacat
     2761 aggggcaatg ataaaaggtg gcaaaaatga atgtttccag tagaactgta gtactgataa
     2821 atgtctttgc tgctgttggt ttgtttactc ttatctctat gagatttggc tggtttat
//
LOCUS       DAJTZL010000305         2869 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.308, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000305 DAJTZL010000000
VERSION     DAJTZL010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2869
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.308"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..116)
                     /locus_tag="OO544_004170"
     CDS             complement(<1..116)
                     /locus_tag="OO544_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HCR6769617.1"
                     /translation="MEVNKKQLADIFGASIRTIQNWQEQGMPVLRGGGKGNEV"
     gene            529..1596
                     /locus_tag="OO544_004171"
     CDS             529..1596
                     /locus_tag="OO544_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001383880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta family protein"
                     /protein_id="HCR6769618.1"
                     /translation="MSQHQYYPQLKWKPAEYESLMLLDQTTLSGFTPIITIPDIDWDY
                     ENECYKKSLSSYLSDFGINLAASWKANRPVLLDVKYLDKHGSSRHHPLDMCIQDARVN
                     GKEIIPVVSPAYSTNYIHAVQRNLINGLAISITPQTWHQFTSLVNHLNIHPSLIDVII
                     DFGDIQNATDSLKQQALSMVNTLSGQAPWRNLILSSTAYPASQAGIPQHQVHHIPRHE
                     YDLWMYVVQNFSNGRTPSFSDYPTASSTITSVDPRFMSQYVSVRYSNDTSWIFVKGTA
                     VKGNGWGQTKNLCTTLVSSPEYHVFGSKFSWGDDYIYQRSLGANKSGGSKEWRKVAHT
                     HHITLVVRQLYWLAQTQPAKP"
     gene            complement(1538..2158)
                     /locus_tag="OO544_004172"
     CDS             complement(1538..2158)
                     /locus_tag="OO544_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000654791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7726 family protein"
                     /protein_id="HCR6769619.1"
                     /translation="MKDQDVRFAVHHKLLKESHLDPDCLVVDEFSISLGASRADIAVI
                     NGVIHGYELKSEYDSLERLPLQIKHYSSVMDKVTLVVAEKHLEGALKLIPGWWGVKTV
                     SVGPKGAILIKHMRGEKLNRNHNSLMLAQLLWKDECIDVLERWGYSKGIKSKPRFELW
                     NIIAENIPIANLRLEVRTALKKRVGWKVKAWQAESAPTNKAVSRLT"
     gene            complement(2575..>2869)
                     /locus_tag="OO544_004173"
     CDS             complement(2575..>2869)
                     /locus_tag="OO544_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005760024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HCR6769620.1"
                     /translation="KRQRDVAELDARYTKELADANATIESLRADVSVGRKRLQVAATC
                     AKSTTGASGMGNGESPGLTADAELNYYRLRSGIDKITAQVNYLQEYIRTQCLK"
BASE COUNT          809 a          674 c          563 g          823 t
ORIGIN      
        1 acctcattac ccttgccacc gcctcgcaga acgggcattc cctgttcctg ccagttctga
       61 atggtacgga tactcgcacc gaaaatgtca gccagctgct ttttgttgac ttccattgct
      121 cattccacgg acaaaaacag agaaaggaaa cgacagaggc caaaaagctc gctttcagca
      181 ccagtcgttt cctttctttt cagggggtat tttaaataaa aacattaagt tacgacgaag
      241 aagaacggaa acaccttaaa ccggaaaatt ttcataaata gcgaaaaccc gcgaggtcgc
      301 cgccccgtaa cctgtcggat cgccggaaag gacccgccaa cgactttcgc gtgcaggcat
      361 taaaaatttt gcagttccat gcctagttga aacctcgatt tctataacat ccaattttgt
      421 aaatttagat atagctcaac ttttcccaat gttttcaagt gtataaaaac aattggcgtt
      481 acgccataac actatactta ggataagtaa agattttaag gagttttaat gagtcaacat
      541 caatattacc cacagctgaa atggaagcct gctgaatatg aatctctgat gcttttagat
      601 caaactacgc tctctggttt tactccgatc attaccattc cagacataga ctgggattat
      661 gaaaacgaat gctacaagaa gagtttgagt tcttacttat ctgacttcgg tattaacctt
      721 gcggcatcct ggaaagccaa tcgtcctgtt ttgctggatg ttaaatattt agataaacat
      781 ggttcgagcc gccatcatcc tctagatatg tgtatccaag atgctagagt aaatggtaag
      841 gaaattatcc ctgttgtctc tcccgcatat tcaacaaact atatacatgc tgttcaacgc
      901 aacttaatca atgggctcgc tatatctatc accccccaga catggcacca attcacaagt
      961 ctggttaacc acttaaatat tcatcctagt ttaattgatg taatcattga ttttggagat
     1021 attcaaaacg caactgatag tttaaaacaa caagcattaa gcatggtcaa cacattatca
     1081 ggccaagctc cgtggagaaa cttgatttta tcttcaaccg cataccctgc atcacaggca
     1141 gggataccgc aacatcaagt tcatcatatt ccgcgccatg aatacgatct ttggatgtat
     1201 gtagtacaga attttagcaa tggaagaacg ccaagtttta gtgattatcc caccgctagc
     1261 tctaccatta cgagcgtaga cccacgcttc atgtctcagt atgtctcagt gagatattcg
     1321 aacgatacct catggatctt tgtaaaaggt accgcagtta aaggaaatgg atggggccaa
     1381 actaaaaact tatgtactac ccttgttagt tcgccagagt atcacgtctt tggctccaaa
     1441 tttagttggg gggatgatta catttaccaa agatcattag gcgctaacaa atctggcggc
     1501 tctaaagaat ggcgtaaagt tgcacatacg caccatatta cgttagtcgt gagacagctt
     1561 tattggttgg cgcagactca gcctgccaag ccttaacttt ccagcctacg cgtttcttta
     1621 aggctgttct gacttcaagc ctgagattcg ctattggaat attttccgca ataatattcc
     1681 ataactcaaa tcggggcttg cttttgattc ctttggaata gccccatcgt tcaagtacgt
     1741 cgatacattc atctttccaa agcaattgag cgagcatcaa tgagttatgg tttcgattaa
     1801 gcttttctcc acgcatgtgc tttataagaa tggcgccttt tggcccaaca gaaaccgttt
     1861 taacgcccca ccaacctggg attaacttta atgctccctc aagatgtttc tcagctacga
     1921 caagagtaac cttgtccatt acagaagaat aatgcttgat ttgaagaggc aaacgctcca
     1981 aagagtcata ttcacttttg agctcgtacc cgtgtataac accatttatt acagcaatgt
     2041 ctgctctact ggcgccaagg gatatggaaa attcatcgac cacaaggcag tcaggatcta
     2101 aatgcgattc tttcaaaagc ttatgatgca ccgcgaacct aacatcttga tctttcatga
     2161 cttctcctta ctccctccgt tactgaatta tagcgcactg cattttactg catttgagag
     2221 atttgaccac ttcaatcaca aaatgcttat ctgcagcaac aagattcata gccatacgac
     2281 tatggtgatt accttttgtc tggaaaactg accattccac gaagatcaga taggtctata
     2341 gaatcttggt ttgtcatagc gttcgcctta ctttgagatg aacctttgct gcataggaga
     2401 tcagcccgtc aaggctcacc agcactaact gactactcaa aggctcattc caaagggttt
     2461 ggttcgacgt ggttgagtgc gctgcggtgc gcggtgaaat acctgtacaa aaatgccccg
     2521 catctgcgag gcattttcct gaaagtcact tgttaaattt cagtgaaatt aaaattattt
     2581 taagcactgc gtcctgatgt actcctgcaa gtagttaacc tgcgcggtta tcttgtcgat
     2641 tccacttcgg agacggtaat aattgagttc agcatctgct gtaagtcctg ggctttctcc
     2701 attgcccatg ccgctggctc cggtcgttga ctttgcacag gtggcggcga cttgcaggcg
     2761 cttacgccca acagaaacat cagcacgtag actttcgata gtcgcgttag catcagcaag
     2821 ctcctttgtg tatctggcgt cgagttctgc tacatcacgt tgacgcttc
//
LOCUS       DAJTZL010000306         2826 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.309, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000306 DAJTZL010000000
VERSION     DAJTZL010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2826
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.309"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..474
                     /locus_tag="OO544_004174"
     CDS             <1..474
                     /locus_tag="OO544_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001599808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6769621.1"
                     /translation="THSASASSTDLGTKTTSSFDYGTKSTNNTGAHTHSLSGSTNAAG
                     NHSHRDGRRFNPSVFKDTYQYGYTSSGQNTWGVQGSVGMSTGWLANTSTDGNHSHSLS
                     GTAASAGAHAHTVGIGAHTHSVAIGSHGHTITVNAAGNAENTVKNIAFNYIVRLA"
     gene            636..1058
                     /locus_tag="OO544_004175"
     CDS             636..1058
                     /locus_tag="OO544_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR6769622.1"
                     /translation="MAVFNSDEASWHLVEDHRGKTVYDVASGDALFISELGSLPENVT
                     WLSPEGEYQKWNGTAWVKDTEAEKLFRIREAEETKNNLMQVASEHIAPLQDAADLEIA
                     TEEENSLLEAWKKYRVLLNRVNTTTAPDIEWPTNPVRE"
     gene            complement(1418..>2826)
                     /locus_tag="OO544_004176"
     CDS             complement(1418..>2826)
                     /locus_tag="OO544_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6769623.1"
                     /translation="AEQLAPTNPFNLYQHLFSNRDFDLYLENGDWEERRNKLESRRES
                     AISEILRQDGAEGVIRFADHVSAPHQIGSVLGTINDDVFEKLLLPRFLNTKENKYKAL
                     VSAFIWKRFYLKGWEWFDNLDKADWTPEQLGQFFAYLPFNKETWTRVSHYPQASENEY
                     WARTDANPDLGEGDLTSAIDKLIDHGRPLAAINCLCRMHQTKQPIDTEQCIRALLAAT
                     STNESSSNMDGYNITTLIQFLQTDPKVNPDNLFQIEWAYLPLLDGHQDAVPKFLENKM
                     ANDPEFFCEVIQLIYRSDKEDNMTSEPTDELKAIAKNAWTLLYNWKTPPGSQSNGTFN
                     GERFTEWLQKVKEGSTASGHLDVALSKVGEVLIYTPSDPNGLWINREVAAALNDRDAE
                     SMRRGYMTGAYNSRGVYCVDPTGKPERELADQFRAKAEEVENAGFQRFAVTLRDLAVG
                     YDHEAAQVISNLNIGEDD"
BASE COUNT          760 a          632 c          668 g          766 t
ORIGIN      
        1 acccacagcg ccagcgcatc cagtacggat ttggggacga aaaccacatc gtcgtttgat
       61 tacggcacta aatccacgaa taacactggt gcgcataccc atagtttaag tggcagcacg
      121 aatgcagctg gtaatcacag ccatagagat ggccgtcgat ttaaccccag tgtttttaaa
      181 gatacttatc aatatggtta tacaagctca ggtcaaaata cctggggtgt acaaggctca
      241 gtaggtatgt ctacggggtg gttagctaat accagtacag atggtaatca tagccactca
      301 ctgtccggca cagcagcatc tgcaggtgca cacgcgcata ctgtcggtat tggtgctcat
      361 acgcactccg ttgcgattgg ttcacatgga cacaccatca ccgttaacgc tgctggtaac
      421 gcggaaaaca ccgtcaaaaa catcgcattt aactatattg tgaggcttgc ataatggcat
      481 tcagaatgag tgaacaacca cggaccataa aaatttataa tctgctggcc ggaactaatg
      541 aatttattgg tgaaggtgac gcatatattc cgcctcatac aggtctgcca gcaaacagta
      601 ccgatattgc accgccagat attccggctg gctttgtggc tgttttcaac agtgatgagg
      661 catcgtggca tctcgttgaa gatcatcggg gaaaaaccgt ctatgacgtg gcttccggcg
      721 acgcgttatt tatttctgaa cttggctcgt taccggaaaa tgtcacctgg ttatcgccgg
      781 aaggggaata tcagaagtgg aacggcacag cctgggtgaa ggatacggaa gcagaaaaac
      841 tgttccggat ccgggaggcg gaagaaacaa aaaacaacct gatgcaggta gccagtgagc
      901 atattgcgcc acttcaggat gctgcagatc tggaaatcgc aacggaggaa gaaaactcgt
      961 tgctggaagc ctggaaaaag tatcgggtgt tgctgaaccg tgttaataca acaactgcac
     1021 ctgatattga gtggcctacg aaccctgtca gggagtaatc attgggatta tgccgcagaa
     1081 acgttgtatg caataacgtt ttgcggttgg ctggtgaact tccgatagtg cgggtgttga
     1141 atgatttcca gctgctaccg attttacata tttttgcctg agaggatttg aaccgcctcc
     1201 acagactcta catcacagat aaccactgtc acgaggactg ttagtgctgt gagttcaacg
     1261 gggcgatgta cgctaccttt aatcaagagg gagaggtacg ttgctaagat gagtttatat
     1321 tcatcaacta agtgcagatc agcgaataac taaaacttgg ctgtctgtga caggaacatc
     1381 gattgacagc cagtttctcc acatttaggt gtttagatta atcatcctct ccaatattta
     1441 gattgctgat aacctgtgca gcttcgtggt catagccaac cgctaaatct cttaaggtaa
     1501 cggcgaatcg ctgaaaacca gcgttttcca cttcttctgc ttttgcccgg aactggtcag
     1561 ctagctctct ctcaggtttt cctgttggat caacacaata aacacctcgt gagttgtagg
     1621 ctcctgtcat gtagcccctg cgcatactct cagcatctcg atcgttgaga gcagctgcga
     1681 cctctcggtt tatccacaag ccattcggat cggagggagt ataaatcaag acttcaccaa
     1741 ctttgctgag tgcaacgtcc aggtgacctg atgccgtaga accctcctta actttctgaa
     1801 gccattcagt aaagcgttct ccgttgaacg tcccatttga ttggcttcct ggcggtgtct
     1861 tccagttgta caaaagagtc caggcatttt ttgcgattgc tttcaattca tctgttggtt
     1921 cacttgtcat attgtcttct ttgtcagaac gataaatgag ttggattact tcgcagaaaa
     1981 attctggatc attagccatt ttattctcaa ggaacttagg cacagcatct tggtgaccat
     2041 cgagcaatgg aaggtaagcc cactcgatct gaaaaagatt atccggattc actttgggat
     2101 cagtctgaag aaattggata agggtagtta tgttgtaacc atccatgttg gagctagatt
     2161 cgttagttga agtggcagca agcaaagcgc ggatacactg ttcagtatcg attggctgct
     2221 ttgtctgatg catcctgcac aggcaattga ttgccgcaag tggtctgcca tgatcaatca
     2281 gcttatcgat ggcactagta agatcacctt cacccaggtc aggatttgca tcggtgcgag
     2341 cccaatattc gttctcggaa gcttgaggat aatgagagac tcgggtccag gtctccttgt
     2401 taaaaggcag ataagcaaag aattggccta actgttcagg agtccagtca gctttatcca
     2461 gattgtcgaa ccactcccag cctttgagat agaaacgctt ccaaataaaa gcgcttacca
     2521 atgccttata cttattttct tttgtattga ggaaacgggg caaaagaagt ttttcgaaca
     2581 catcatcgtt gatagtgccg agtacacttc ctatttgatg gggcgcagaa acgtgatctg
     2641 caaatcgaat aacgccttct gcgccatctt gcctaagaat ttctgatatt gcggattcgc
     2701 gccttgattc gagtttattt ctccgttctt cccaatcgcc attttctaag taaagatcaa
     2761 aatctcgatt tgaaaaaagg tgctgatata agttgaatgg attggtaggt gccagttgct
     2821 cggcaa
//
LOCUS       DAJTZL010000307         2814 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.310, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000307 DAJTZL010000000
VERSION     DAJTZL010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2814
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.310"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..989
                     /locus_tag="OO544_004177"
                     /pseudo
     CDS             1..989
                     /locus_tag="OO544_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480735.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
     gene            1005..1754
                     /locus_tag="OO544_004178"
     CDS             1005..1754
                     /locus_tag="OO544_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCR6769624.1"
                     /translation="MRYLNTKNLIAAGVLLACMSSIAWGAIIPDRTRIIMNESDKGEA
                     LKLTNQSKNLPYLAQTWIEDTKGNKSRDFIVTVPPMVRLNPSEQIQIRMISQEKIAQL
                     PNDRETLFYFNVREIPPKTDKKNVMQVTMQHALKLFWRPKAIELEDDGVMSYEKVEII
                     RRNDGSIRFNNKMPYHVTLGYIGTNGITMLPQTQSLMVTPYSHADTQFKNVPSAFQVG
                     YINDFGGLSFYEINCPTVNNSCNVSVAKRDK"
     gene            1756..>2814
                     /locus_tag="OO544_004179"
     CDS             1756..>2814
                     /locus_tag="OO544_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6769625.1"
                     /translation="MRYLLIVIIFIIGFSALPVWAMDCYAEHEGGNTVVIGYVPRISI
                     PSNGKKGDKIWQSSEYFMNVFCNNALPAPSPGEEYPSAWTNIMMFLPGGQDFYNQNSY
                     IFGVTYNGVDYDSTAPNAIAAPECIDIKGAGTFNNHYKNPAVCSGGPEPQLSVTFPAR
                     VQLYIKLAKNANRVNKNLVLPDEYIALEFKGMSGAGAIEVDKNLTFRIRGLNNIHVLD
                     CFVNVALEPADGVVDFGKINSRTIKNTSVSETFSVVMTKDPGAACTEQFNILGSFFTT
                     DILSDYSHLDMGNGLLLKIFHKDGTATEFNRFSQFASFSSSSAPSVTAPFRAELSANP
                     AETVVEGPFSKDVILKITY"
BASE COUNT          885 a          566 c          585 g          778 t
ORIGIN      
        1 aggttaaata catacgtaag tttggctcgt aatacttact gggacgccag cagtaatgtg
       61 aattattcat tatcacttag ccgcgatttt gatatcgggc cattaaaaaa cgtctccgct
      121 tctctaacat ttagccgaat aaactgggaa gaagacaacc aggatcaact gtacctaaat
      181 atttcgattc cctggggaac cagtagaaca ttgagctatg gtatgcaacg aaatcaggat
      241 aacaagattt cgcatactgc ttcgtggtat gactcttccg atcgaaataa ttcctggagc
      301 gtttctgctt caggcgacaa tgatgaattc aaagatatga aagcgtcact acgcgccagt
      361 tatcagcata ataccgagaa cggtcgtctc tatctctccg ggacatcaca gcgagacagt
      421 tattattctc tgaatgccag ttggaatggt tcattcacgg cgactcgtca cggagccgct
      481 ttccatgact atagtggtag tgctgactcg cgttttatga tcgacgcaga tggtgcagaa
      541 gatattccgt tgaacaataa acgcgcggta actaatcggt atggcatcgg agttattcca
      601 tcagtcagca gttacataac aacatcatta agcgttgata cccgaaatct tccagaaaat
      661 gtggatatcg aaaactcggt tatcaccacc accttaaccg aaggtgctat tggctacgcc
      721 aaacttgata ccccaagggc tatcaaatca tgggggttat tcgcctggca gatggtagtc
      781 acccaccact ggggattagc gtaaaagata aaaccagcca taaagaatta ggactggttg
      841 ctgatggcgg ctttgtatac ctcaacggca ttcaggatga cagcaaactt actttacgct
      901 ggggtgataa atcctgtttt attcaaccac ccaatagcag caacttaacc accggaacgg
      961 ttattttacc gtgtattagc caaaattaat tcaggatgaa actaatgcgc tacttaaaca
     1021 caaagaatct cattgcagca ggagtattac tcgcctgcat gagcagcatt gcctggggtg
     1081 ccatcatccc ggaccgtacc cgtatcataa tgaatgagtc agataaaggg gaagcattaa
     1141 aacttactaa tcaaagcaaa aatctaccct atctggcgca aacatggata gaagatacga
     1201 aaggcaataa atcacgtgac tttattgtca cggttccccc tatggtacgt ttaaatccca
     1261 gtgagcaaat tcaaataaga atgataagtc aggaaaaaat tgctcaactg cctaatgaca
     1321 gagaaacgct cttctatttt aacgtgcgag aaataccgcc aaaaacagat aaaaaaaatg
     1381 tgatgcaagt cactatgcag cacgcattga aactattctg gcgaccaaaa gctattgaat
     1441 tagaagatga tggggtaatg tcttacgaaa aagttgaaat catccgtaga aatgatggtt
     1501 cgatccgctt taacaataag atgccttatc acgttacgct gggttatatc ggaacaaacg
     1561 gaatcacaat gttgccacaa acgcaaagtt taatggtaac cccttatagc catgcagata
     1621 cgcagtttaa aaatgttccg tcagcttttc aggttggtta tatcaatgat tttggtggat
     1681 taagttttta tgaaataaac tgcccgacgg ttaataactc ttgtaatgtt tccgtagcca
     1741 aacgagataa ataatatgcg ctacttatta attgtaatta tatttattat aggttttagt
     1801 gcattacctg tatgggcaat ggactgctat gctgaacatg aagggggaaa taccgtagtc
     1861 attggttacg taccaagaat ttctatcccc agtaatggta aaaaaggtga taagatctgg
     1921 caaagcagtg agtattttat gaatgtcttc tgtaataatg cattacctgc cccatcacct
     1981 ggagaagaat acccatcagc ctggactaat ataatgatgt ttttaccagg cggtcaggac
     2041 ttttataatc agaactccta tatttttggc gtaacataca atggagttga ttatgattcg
     2101 actgccccta atgctatcgc agcaccagaa tgcattgata taaaaggagc gggtaccttt
     2161 aataatcact ataaaaaccc cgctgtctgt agtggaggtc ctgagcctca attatcagta
     2221 acctttccag ctcgcgtaca actctatatt aaattagcaa aaaatgccaa tagagttaat
     2281 aaaaacctgg tgttacctga tgaatatatc gctctagagt ttaaaggtat gagtggcgca
     2341 ggagctatag aagtagacaa aaatttgacc ttcaggattc gtggattaaa taatattcat
     2401 gtccttgact gctttgttaa tgtcgcactg gaaccagctg atggcgttgt tgactttggt
     2461 aaaataaatt cccgaacaat taaaaatacc agcgttagtg agacgtttag tgtagtcatg
     2521 acaaaagatc ctggtgcggc ctgtactgag caattcaata ttttagggag ctttttcact
     2581 acggatattt tgagtgatta tagccatctg gatatgggta atggactgct attgaagata
     2641 tttcataaag atggaacagc aacggaattt aaccgcttct cacaatttgc ttctttttca
     2701 tcgtctagtg caccttcggt caccgcacca ttcagggcag aacttagtgc gaacccggca
     2761 gagactgttg ttgagggacc gtttagtaaa gacgtaatcc tcaaaatcac ctat
//
LOCUS       DAJTZL010000308         2797 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.311, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000308 DAJTZL010000000
VERSION     DAJTZL010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2797
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.311"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..617
                     /locus_tag="OO544_004180"
                     /pseudo
     CDS             1..617
                     /locus_tag="OO544_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3289 family protein"
     gene            614..1093
                     /locus_tag="OO544_004181"
     CDS             614..1093
                     /locus_tag="OO544_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF943 family protein"
                     /protein_id="HCR6769626.1"
                     /translation="MKSKLQKIILCLFLLCCIYNLWTLRPVQILYTYSDAGNSVFLVV
                     DHLPWTDSDKINWYLKHQNEIKNQHPLPEGSWHTWYVIDIGNGFTDYKKYIEGPYEDL
                     YCFPTIKSNDNCIVKNYLMVINEYPYRNTHIGINDFTEYQLTQENKIERVFNPHDFK"
     gene            complement(1147..2097)
                     /gene="corA"
                     /locus_tag="OO544_004182"
     CDS             complement(1147..2097)
                     /gene="corA"
                     /locus_tag="OO544_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HCR6769627.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            2467..>2797
                     /locus_tag="OO544_004183"
     CDS             2467..>2797
                     /locus_tag="OO544_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6769628.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLALTLLPLFAVAADDCALSDPMLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLVDMNSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQ"
BASE COUNT          842 a          620 c          591 g          744 t
ORIGIN      
        1 tccggaacaa aaatatcaca acgtgaatgc actgatattt tatttgcaga aatgaaagaa
       61 ctagcaaaaa tgttttcctt ttttggacaa tataaaacac ttatcgaaga tctgattgaa
      121 cattttagat atggaaatgg cagcaatttt catagccaac aattaaattt atcttttcat
      181 gaaaaaataa atatggttat aatagtccaa tacgaataat caaagaatgt atcgaaaatg
      241 gcattaattc aactccctcg acaggatacc aacctcttat cctgcaaagc attaaaacca
      301 aattactttc atcaagacta aataaattta atgacttcga ggacagtttt aatggcttag
      361 gaatcagtgt tcatgatatt tcagctcaaa aaatatctct tttaagtttt caaaaatacg
      421 ctataggatg gagtgcaaca attcattttg tagcacagga ccacttcggt cttgatgtaa
      481 ctgatatcaa aaacaagact tacagcaaat atagattttt tcgcatatgg ttctttttac
      541 aacggcataa agactttgct ttcaaacctt ttttcaccaa ttttaacact atcgaaagaa
      601 tcgaaaatta tctatgaaaa gcaaactcca gaaaattata ttatgtttat ttcttctatg
      661 ctgcatctat aatttatgga cattgcgtcc tgtacagatt ttgtatacat actctgatgc
      721 tggcaattca gtctttctgg tcgttgatca tctgccatgg actgacagcg ataaaataaa
      781 ttggtatctg aagcatcaga atgaaattaa aaatcaacat cccctaccag aaggtagttg
      841 gcatacctgg tatgtcattg atatcggtaa tggcttcact gactataaaa aatatatcga
      901 agggccgtat gaagatctct actgtttccc gacaattaag agcaatgata attgcatcgt
      961 gaaaaattac ttaatggtta ttaatgaata cccttatcgt aacactcata ttggtattaa
     1021 cgattttact gagtatcaat taacacaaga aaataaaata gagcgagttt tcaatccgca
     1081 tgactttaaa taagacaatt tttagaaata agccggataa aaaaatagct ttatccggca
     1141 ctcatattac agccagttct tgcgcttaaa gtacagatac ggtgccaggc ccgcgaggat
     1201 cataaagata atcgcgcctg ggtagccgaa actccacttc agttctggca taaactcaaa
     1261 gttcatccca tagctggaag caacgagcgt cggcggcagg aataccacgg ataccaccga
     1321 gaagattttg atgatgcggt tctgctcgat gttgataaaa cccatcgccg cctgcatcag
     1381 gaagttcacc ttctggaaca gggattcgtt atgcggcagc agggattcga tatctcgcag
     1441 gatttcacgc gcctgctcca gttgcccacc cggtaaacgc gctttacgca ccaggaagtt
     1501 gagcgcgcgc tgggtatcca tcagacacag gcgaactttc cagccgatgt cttccagttc
     1561 cgccagagtg gagagcgcct cgtcgtactc atcgccctga tgcccttcca taatcacccg
     1621 gctcaactgc tccaggtcgc tatagatatt ttcaatttca tctgccaact gttcgatttt
     1681 ggtttcgaac agatccagca gcagctcgta ggcgttaccg tctaccatcg actggctacg
     1741 ggcacgcata cgatacagac gaaaagcggg cagttcacgc tcacgcagag taaacagacg
     1801 accatcacgg atggtaaatg ccacagtgga gttaccggcg tgatcttccg catcttcaaa
     1861 gaagaagaag gagtgaatat gcaggccgtc gtcgtcttca aagaaacgtg ccgatgcttc
     1921 gatgtcttcc agttccgggc gggttgccag gctctggcca agttcagatt gtacgcgcag
     1981 tcgctcgtcg tcgtccggtt cgacaagatc aatccatact gcatttacaa ggggttgtga
     2041 ctcttcgact tccagccggg tcagtcggtt attttccagt tgaaatgcgc tcagcatgac
     2101 cgggactccc aatgcttaaa atatcggaca gttcggtggg cacacagaaa caaattgggt
     2161 ttcagaccat taaacagcct gactcagcgc gacgggaaaa attgaggtcg ctgacaaccg
     2221 ctaaggctat cagcaaaaag ggatagcctt aggagttgat cctggatgac aggatagtga
     2281 gccagtatct actgggtgtg tccaaggcga atgtcctctt agagtgatcg tgcgcgcatg
     2341 ttacgccagc aaaattttgc cgtcaacacg caacagaaca ccacagaaca atagttgaac
     2401 ttcgcgcttt ttggtactcg tcaaggtcgc taacaggata aaaaaatcca acatccagga
     2461 cctataatgg cgcatcggct actcattggt aaaggaatga tcaccttgaa tctcaaacgg
     2521 atttttctcg ccctcacctt acttccctta tttgctgttg ccgctgatga ttgcgcactc
     2581 tcagatccga tgctgaccgt acaggcgtat accgttaatc ctcaaacaga gcgggtgaaa
     2641 atgtactggc aaaaagccaa tggcgaagcg tggggaactt tacatgctct gctggtggat
     2701 atgaatagtc agggtcaggt gcagatggcg atgaacggcg gcatctatga tgaaagctat
     2761 gcgccgctcg gtttgtacat cgaaaacggt cagcaga
//
LOCUS       DAJTZL010000309         2739 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.312, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000309 DAJTZL010000000
VERSION     DAJTZL010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2739
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.312"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..1007)
                     /gene="shiA"
                     /locus_tag="OO544_004184"
     CDS             complement(<1..1007)
                     /gene="shiA"
                     /locus_tag="OO544_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
                     /protein_id="HCR6769629.1"
                     /translation="MDSTLISTRPDEGTLSLSRARRAALGSFAGAVVDWYDFLLYGIT
                     AALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVW
                     MMGIATALIGILPSFSTIGWWAPILLVTLRVIQGFAVGGEWGGAALLSVESAPKNKKA
                     FYSSGVQVGYGVGLLLSTGLVSLISMMTTDEQFLSWGWRIPFLFSIVLVLGALWVRNG
                     MEESAEFEQQQHYQAAAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNY
                     STQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFGRRRVYITGALIGTLSAFPFF
                     MA"
     gene            1322..2374
                     /locus_tag="OO544_004185"
     CDS             1322..2374
                     /locus_tag="OO544_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HCR6769630.1"
                     /translation="MFLASLLRRIAFSYYDYKAYNFNIEKTDFVVIHIPDQIGDAMAI
                     FPVIRALELHKIKHLLIVTSTINLEVFNALKLEQTKLTLVTMTMQDHATLKEIKDLAK
                     NITQQYGTPDLCIEGMRKKNLKTMLFISQLKAKTNFQVVGITMNCYSPLCKNASRMDQ
                     KLRAPVPMTWAFMMREAGFPAVRPIYELPLSEDVLDEVREEMRSLGSYIAFNLEGSSQ
                     ERTFSLSIAENLIAKIQSETDIPIVIVHGPKGEDKARALVDCYNNVYRLSLPPSIKRS
                     AAIIKDAYIAITPDTSILHMASAYNTPVVAIYADYKTRWPAMADVSESVVVGQKIDNI
                     SLDEFAKALKSVLARI"
BASE COUNT          849 a          592 c          616 g          682 t
ORIGIN      
        1 gccataaaga aaggaaatgc gctcaacgtt ccgattaacg cacctgtgat ataaacccta
       61 cggcgaccaa aacgatcggc aagccaggca aaacagggaa ttgtcaggca gcttaatcca
      121 cctaccagca aaccaatatt aaggaaaagt tcgcgcggta gccccatatt ctgggttgaa
      181 taattaagtg caaaggcagt aacgatgtac atcgtcagca attcgcacag tcgtagcgca
      241 ataatcttca ggaaagcacc gggatgtcgt aacagcgctt cgataaccgg gatgcgtttt
      301 ttcgcggcag cttgataatg ttgctgttgt tcaaattccg cggactcctc catgccattg
      361 cgcacccaca atgctcccag taccagtacg atgctaaaca ggaaaggaat gcgccagccc
      421 cagcttaaaa actgttcgtc agtcgtcatc atactgatca atgaaaccag tccggttgaa
      481 agcagtaaac ctacaccgta gccaacttgt acaccgctac tgtaaaaggc ttttttattt
      541 ttcggtgcac tttcaacgga aagcaacgcc gcgcctcccc attcgccgcc gacggcaaat
      601 ccctgaatga cacgcagtgt caccagtaaa ataggtgccc accacccaat ggtcgagaat
      661 gaaggaagaa taccaatcaa ggctgtcgcg atgcccatca tccagacggt cagcattaac
      721 atgcgcttac gtcccagtcg gtcgccaaag tgaccgaaaa tgacaccgcc gagcggacgg
      781 aaaaggaatc cgacgccaaa ggtagcaaat gcggcgagcg ttcccatcgc cgggcttact
      841 tgcgggaaaa actcgcgatt aaacaccagt gcggcggtga tgccatagag taaaaaatca
      901 taccagtcga cgacggcacc agcgaagctg cctaacgcag ctcgtcgggc gcgacttaac
      961 gaaagcgtcc cttcatcggg acgagtggag atgagcgtgg agtccataat gatcctgttt
     1021 gtactgtctt tttataatta gtatggaaaa ggtaactcac attgccgtcg atctataaac
     1081 gaaccttatg agactacctc gacagagcgc atctgcaaat gattttatgg ctgattagcg
     1141 gcaacatgaa aaattcgatg gaagatgaaa gcgcaggagt tgctggtgtt gtttaaggat
     1201 taagcagagg acccacaggt tagagatatg aaacataaaa gtccgcatat gcacaaaatg
     1261 gcaccctgat gagtctgata gactgcattg cattataacc agtagggagg cgaggttagg
     1321 tatgttttta gcatcattgt tgagacgtat tgcatttagt tactacgatt ataaagctta
     1381 taatttcaat attgaaaaaa cagactttgt tgtcatccat attcccgatc agattggcga
     1441 tgctatggcc atctttcctg ttattcgggc gcttgaattg cataaaatta agcatctttt
     1501 aattgtaacg tcgacaatta atttagaagt ctttaatgcg cttaaacttg aacagactaa
     1561 attaacatta gtcacaatga ctatgcagga tcacgcaaca ttaaaagaaa taaaagattt
     1621 agcaaagaac ataacacagc aatacggtac gccggatctt tgtattgagg ggatgcgtaa
     1681 aaagaacctg aaaacgatgt tatttatcag tcagttgaaa gcaaaaacga attttcaggt
     1741 tgttggtata accatgaatt gctactcccc tttgtgcaag aacgcgtccc gtatggatca
     1801 gaaactccgg gctcccgtac ctatgacatg ggcatttatg atgcgtgagg cgggttttcc
     1861 agcagtcagg ccaatatatg aattgccact aagtgaggat gtactcgatg aggtgcgcga
     1921 ggaaatgcga tcgttaggat cttacattgc gttcaattta gaaggtagct cgcaggaacg
     1981 tacattttca ttatcgattg cagaaaatct aatagcaaaa attcaaagtg aaacagatat
     2041 accaatagtg atcgttcatg gacccaaagg tgaagataaa gccagggcat tagtggattg
     2101 ttataataat gtctaccgtt tatccttacc accctcgatt aaacgttcag cagcaatcat
     2161 aaaagatgct tatatcgcaa taactcctga cacctcaata ttacatatgg caagtgccta
     2221 taatacccct gttgttgcaa tttatgctga ttacaaaacg cgatggcccg caatggccga
     2281 tgtctcggag tcagtcgtcg ttgggcaaaa aattgacaat ataagtctgg atgaattcgc
     2341 aaaggcatta aaaagtgttt tggcgagaat atgagacagt acttatgcat agtaatgccg
     2401 atctgttatg aacggtggtg tcatcaatac tctgaggctg gtcaatcatc acagaaaacc
     2461 agcctttgaa tcaaagcagt actcaccgga tacgatcata gtcacatcga acgcaattct
     2521 ggcggtgccg catttttatt tattcatttt ctgtgagaga agggcaagct ctatagaggg
     2581 aatgtaaaaa agagagctcc gaatggagca ctctttaaga aattataacg gaaaatcaga
     2641 ggtttttata acatgtcgca taagcaaacc caagcttttc aaatacgctt tgatcacctg
     2701 ttgccaggga aactacgtat tgttcgcttg agtttgcgg
//
LOCUS       DAJTZL010000310         2729 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.313, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000310 DAJTZL010000000
VERSION     DAJTZL010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2729
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.313"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..686
                     /locus_tag="OO544_004186"
     CDS             <1..686
                     /locus_tag="OO544_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001463032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA helicase"
                     /protein_id="HCR6769631.1"
                     /translation="PAFDRNGKSAGIWLNPLTTDDGNGLRGFSGEGRVKGSGDAQFVA
                     LQGSRNGESLLADNMQDGVRIARDNPDSGVVVSIAGEGRPWNPGAITDGRVWGDIPDN
                     SVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEIVRKMAENKPDLPDGKTELAVR
                     EIAGQERDRAAITEREAALPESVLRESQREREAVREVARENLLQERLQQMERDMVRDL
                     QKEKTPGGD"
     gene            706..1453
                     /gene="traX"
                     /locus_tag="OO544_004187"
                     /pseudo
     CDS             706..1453
                     /gene="traX"
                     /locus_tag="OO544_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
     gene            1508..2068
                     /locus_tag="OO544_004188"
     CDS             1508..2068
                     /locus_tag="OO544_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HCR6769632.1"
                     /translation="MTEQKRPVLTLKRKTEGETPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAAARLDKIRRQNRIKAELQAVLDEK"
     gene            2198..2410
                     /locus_tag="OO544_004189"
     CDS             2198..2410
                     /locus_tag="OO544_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HCR6769633.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGGRAVILEVSGRWMEAAEAWRRAAC
                     VAPRTDWQQFARKRAEHCHRRCRGRV"
BASE COUNT          663 a          646 c          869 g          551 t
ORIGIN      
        1 tgccggcgtt tgaccgtaac ggcaagtccg ccggtatctg gctgaacccg ctgaccacgg
       61 atgacggaaa cgggctgcgg ggattcagcg gtgaaggtcg ggtaaaaggc agcggggatg
      121 cgcagttcgt ggccctgcag ggcagccgta acggagaaag cctgctggct gataatatgc
      181 aggatggcgt ccggattgcc cgtgataatc ctgacagtgg tgtggtggta agcattgccg
      241 gtgaaggccg tccgtggaat cccggtgcca tcaccgatgg ccgcgtgtgg ggggatatcc
      301 cggacaacag cgtccagccg ggagccggaa atggcgaacc ggtcacggca gaggtactgg
      361 cacagcggca ggctgaagag gccatccgcc gtgaaacgga acgtcgcgca gatgaaattg
      421 tccgtaaaat ggcagagaac aaacctgacc tgccggacgg caaaacagag ctggctgtca
      481 gggaaattgc cgggcaggag cgtgaccggg ctgccataac tgaacgggaa gccgcgctgc
      541 cggagagtgt gctgcgtgaa tcacaacggg agcgggaagc ggtccgggag gttgcccggg
      601 aaaatctgct gcaggagcgt ctgcagcaga tggagcggga tatggttcgt gacctgcaga
      661 aagagaaaac cccgggcgga gactgataca ggaagacaaa cactgatgac aacggataac
      721 acgaacacga cacgtaacga ttcactggct gcacggaccg atacctggtt gcagtcattt
      781 ctggtctggt cacccggaca gcgggatatc atcaaaacgg tggcactggt gctgatggta
      841 ctggaccaca tcaatctgat attccagctg aagcaggaat ggatgtttct ggccgggcga
      901 ggagcctttc cgctttttgc cctggtgtgg gggctgaatc tgtcccgtca tgcgcatatc
      961 cggcaaccgg ccattaaccg gctgtgggga tggggaatta ttgcccagtt cgcatattat
     1021 ctggcaggct ttccctggta tgaggggaat atcctgtttg cctttgcagt ggcggcacag
     1081 gtgctgacgt ggtgtgaaac gcgcagtggg tggcgtacag cagcggccat tcttctgatg
     1141 gccctgttgg gggcctttgt ccggcaccag ttacggcatt gccgggctgc tgatgctggc
     1201 agtcagccac cgactgtacc gggcggaaga cagagcggaa cgtctggcgc tggtcgcctg
     1261 cctgctggct gttattccgg cgcttaacct tgccaccagt gatgcagcgg cggtggccgg
     1321 tcttgtgatg acggtgctga ccgttggtct ggtgtcgtgt gcaggaaaat cattaccccg
     1381 tttctggccc ggggattttt tcccggtatt ctacgcctgt catctggctg tgctgggcgt
     1441 tctggcgctg tgacgggtgt ggtatctttg accgtaacag gatgattcgt cagaggcaga
     1501 acacagcatg acagagcaga agcgaccggt actgacactg aagcggaaaa cggaagggga
     1561 gacgcctgtc cgcagccgga aaaccatcat caatgtcacc acgccaccaa agtggaaggt
     1621 gaaaaagcag aagctggccg agaaagcagc ccgggaagca gagctggcgg caaaaaaagc
     1681 gcaggccaga caggcactgt ccatttatct gaacctgccc acgctggatg aggccgtgaa
     1741 taccctgaaa ccctggtggc cgggattatt tgacggtgac accccccggc ttctggcctg
     1801 cggtatccgg gacgtgttac tggaagacgt ggcgcagcgg aatatcccgc tctcgcataa
     1861 aaaactgcgc agggcgctga aggccatcac ccgttcagaa agctatctgt gtgccatgaa
     1921 agccggtgcc tgccggtatg acacggaagg gtatgtgacg gagcatattt ctcaggagga
     1981 agaagcgtat gcggcagcgc gtctggataa aatccgccgc cagaaccgga taaaggcaga
     2041 acttcaggcc gtgcttgatg agaaataaaa aaaagcctcc atcagagagg aggcagggaa
     2101 ataaggctta aaaggaaata acttccgctt ccgaaaggaa gccagtccac tgaattattt
     2161 atcacaataa tactgacaac taaaggggga caagaaaatg aatggattca gaaacagttc
     2221 acggaacggc caggtctggc gttaccagcg tgccggcggg cgggccgtta ttctggaagt
     2281 cagtggacgc tggatggaag cggcagaagc atggcgacgg gctgcctgtg ttgcgcccag
     2341 gacagactgg cagcagtttg cccgaaaaag ggctgaacac tgccaccggc gctgccgggg
     2401 aagggtgtaa cgaaaaaagc agccttcatt actccaggag tcagaaggct gaaaggagtt
     2461 tatgagattt gcttttttta atatccggtc gggcagtatg accggacagt tctgagtagc
     2521 acagagtttt ttccgtatta agtcgtgatt ttatattttg tgattaattt cacaaaataa
     2581 ggtgttattc agtgtgtgct gcaatattca ggatgcctga aatacagatg ctatttatct
     2641 gatatggaga ataacatgag gaaatagtaa ggcagtatga ctttccgtga gacagatgct
     2701 ggccaacgtg ggatatttaa cgcgggtta
//
LOCUS       DAJTZL010000311         2721 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.314, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000311 DAJTZL010000000
VERSION     DAJTZL010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2721
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.314"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            30..2546
                     /locus_tag="OO544_004190"
     CDS             30..2546
                     /locus_tag="OO544_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR6769634.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQTSSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYTFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFDEANTRNWNYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNDKNRNISVGVSGQQWGIGYSLNYQYSRYTDQNNDRALSL
                     NLSIPLERWLPRSRVSYQMTSQKDRPTQHEMRLNGSLLDDGRLSYSLEQSLDDDNNHN
                     SSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHTHGVTLSQYLGNAFALIDAN
                     GASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQFVVPNR
                     GAMVAARFNANIGYRVLVIVSDRNGKPLPFGALASNDETGQQSIVDEGGILYLSGISS
                     KSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            2557..>2721
                     /locus_tag="OO544_004191"
     CDS             2557..>2721
                     /locus_tag="OO544_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6769635.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGRE"
BASE COUNT          778 a          657 c          630 g          656 t
ORIGIN      
        1 caaatataaa aaacacaggt catcagggaa tgccacaacg acaccaccag ggacataaac
       61 gcacaccgaa acagttggcg ctcatcatca aacgttgttt gccgatggtg ctcactggca
      121 gcggcatgct ttgcactacc gctaacgccg aagagtatta tttcgacccc attatgctgg
      181 aaactacaaa aagtggtatg caaacaaccg atctgtcacg tttttcaaaa aaatacgcac
      241 aactaccagg aacttatcag gttgatatct ggctgaataa aaagaaggtt tcacagaaaa
      301 aaattacatt taccgccaat gcagagcaac ttctgcagcc acagtttacg gtagaacaac
      361 tacgtgagct gggtattaag gtggatgaaa tcccggcgct ggctgaaaaa gatgacgata
      421 gcgtgatcaa ctcgcttgaa caaatcattc ccggtacagc agctgaattt gatttcaatc
      481 atcagcgact taatttgagc attccccaaa ttgcactgta ccgtgatgca agaggttacg
      541 tctccccttc tcgttgggac gatggtatac caacgctgtt taccaactac tcgtttacag
      601 gttctgataa ccgttaccgc cagggcaatc gtagccaacg acagtacctg aatatgcaaa
      661 atggtgctaa ttttggcccc tggcgattac gcaactattc cacatggaca cgcaacgatc
      721 agacatcaag ctggaatacc atcagtagtt atttacaacg tgatatcaag gcattgaagt
      781 ctcagttgct tctgggagaa agcgccacca gcggcagtat tttttccagc tacaccttta
      841 ctggcgttca actcgcttcc gacgataata tgttgccaaa cagccagcgc ggatttgccc
      901 caacggtacg cggtatcgca aacagtagtg caatcgtgac tatcaggcaa aatggttatg
      961 tgatctatca aagcaacgtg ccagcgggtg cctttgaaat taacgatctc tacccctctt
     1021 ccaacagcgg cgatttagaa gtcacgattg aagaaagtga cggtacgcaa cgtcgcttta
     1081 tccagcctta ttcttcatta cccatgatgc agcgacctgg gcatctaaaa tatagcgcga
     1141 ccgctggacg ctatcgcgct gatgcaaaca gtgatagcaa agaacccgaa tttgctgaag
     1201 ccacggcaat atatggtttg aataatactt ttacgctgta tggtggcctg ctcggttctg
     1261 aagattatta tgcgctgggg atcggtatcg gcggcacact tggcgcactg ggcgcgttgt
     1321 cgatggatat caacagagct gacacccaat tcgataacca gcactctttt catggctatc
     1381 aatggcgtac gcagtacatc aaagatatcc cggaaaccaa caccaatatc gctgtcagct
     1441 actatcgcta taccaacgat ggctatttta gttttgatga agccaatacc cgtaattgga
     1501 actataacag tcgccaaaaa agtgaaattc aattcaacat cagccagaca atatttgatg
     1561 gggtaagtct gtatgcctcc ggttcgcagc aagactattg gggcaataac gataaaaaca
     1621 ggaatatctc agttggggtt tccggtcagc aatggggaat tggttacagc ctgaattatc
     1681 aatacagtcg ctacacggat caaaataatg accgcgcact ctctttgaat ctcagtattc
     1741 cgttagaacg ctggttaccg cgtagccggg tttcctatca gatgaccagc cagaaagatc
     1801 gcccaaccca acatgaaatg cgtcttaatg gctcactgct ggatgatggt cgcctgagct
     1861 atagcctgga acaaagtctg gatgacgata acaaccataa cagtagcctg aacgccagtt
     1921 accgttcacc ttatggcacc ttcagtgccg gatacagcta cggtaatgac agcagccaat
     1981 acaattacgg cgttaccggc ggcgtggtta tccatactca tggcgtgacg ctctcgcaat
     2041 atctgggcaa cgcttttgcg cttatcgatg ctaatggggc ttctggcgtg aggatacaaa
     2101 actatccggg gattgctact gatccctttg gctatgcagt ggttccttat ctcacgactt
     2161 accaggaaaa ccgtctctcg gtagatacta cgcagctgcc cgataacgtc gatcttgagc
     2221 aaacaacaca gtttgtggtg cccaacagag gtgcaatggt agcggcgcgt ttcaacgcca
     2281 atatcggtta tcgcgtactt gttatagtca gcgatcgcaa cggtaaaccg ttgccctttg
     2341 gcgctcttgc cagcaacgat gagacggggc aacaaagtat cgtcgatgag ggcggcatac
     2401 tatatctctc tgggatatcg agtaaatcac aaagctggac tgtacgctgg ggaaatcagg
     2461 cagatcaaca atgtcagttt gcttttagta caccggattc agaaccaaca acctctgtat
     2521 tacaaggcac ggcgcagtgc cattaaggat aaaaaaatga aaagagcgcc tcttataaca
     2581 ggacttttgt tgatatccac atcctgcgct tatgcctcct caggagggtg tggagctgac
     2641 agcactagcg gtgcgacaaa ttacagcagt gtggttgatg atgttacggt gaaccagaca
     2701 gataacgtga caggacggga g
//
LOCUS       DAJTZL010000312         2690 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.315, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000312 DAJTZL010000000
VERSION     DAJTZL010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.315"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            230..769
                     /locus_tag="OO544_004192"
     CDS             230..769
                     /locus_tag="OO544_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015870862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HCR6769636.1"
                     /translation="MSHGGWKEISHPQTLFTRQKGDGWTVVPKNLRIDFYTKDNDFTK
                     GLSVLSEVNKRHTEAQKGLTPSLNISKDDLKVLANARNIPQSKLEEEMMSQAIYRKEY
                     ATSNEKIKNYALYYHEQAQNIIQRHQDGLGNKNIDIAFITDKNHKKHLSDIFKVINES
                     GVKYDVIHFGACRVNREEK"
     gene            complement(995..1318)
                     /locus_tag="OO544_004193"
     CDS             complement(995..1318)
                     /locus_tag="OO544_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nikA protein"
                     /protein_id="HCR6769637.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLR
                     AAALGKNVNSLTDDRVLKEVMRLGALQKKLFIDGKRIGDREYAEVLIAITEYHRALLS
                     RLMAD"
     gene            1604..1798
                     /locus_tag="OO544_004194"
     CDS             1604..1798
                     /locus_tag="OO544_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HCR6769638.1"
                     /translation="MNKQQQTVLNMAGFIKSQSLTLLEKLDALDADEQAAMCEKLHEI
                     AEELQNSIQTRFEAESGTGV"
BASE COUNT          857 a          665 c          579 g          589 t
ORIGIN      
        1 aaaaaaaaca ataaaaaaca ccgcaaaaat ggattgttaa caaatgaaaa tactcaattc
       61 gaatctcata acaaacatat catcaacaat aagaaatcac cttctaacat catccaaaaa
      121 tataaaaaca cacccatcac caaacatgga cgtaacaagt catcgtcgtt tattccagaa
      181 agacggaata ggattgagcc aaacaggaaa agcaacacaa cttgtcatta tgtcgcatgg
      241 tggatggaaa gagatctccc atccacaaac actattcacg cgtcaaaaag gcgatggatg
      301 gactgtagtc cccaaaaacc taagaattga tttttataca aaagacaacg acttcacaaa
      361 gggactaagc gttctttcag aggtaaacaa acgccatact gaagctcaaa aagggttaac
      421 accatcacta aacataagca aagatgacct aaaagttcta gcgaacgcgc gcaatatacc
      481 tcaatcaaaa ttagaagaag aaatgatgtc acaagccata tatagaaaag aatacgcaac
      541 aagtaatgag aagataaaaa actatgctct atattaccat gaacaagccc aaaatattat
      601 acaaagacat caagatggct taggaaacaa aaatattgat atagcattta taactgataa
      661 aaatcataag aagcaccttt ctgacatatt taaagtaata aatgaatcag gagtaaaata
      721 tgatgtaatt cactttggcg cttgccgagt aaatagagag gagaaataat catcatttac
      781 cataatccct taattgtacg caccactgaa acgcgctgaa cgcgattctg acgcagacac
      841 cacaaaaata gatgttttac cccggaaaaa caggaaggat gcgtcaggat cgttttcagc
      901 tcgctgcata gctatgcatg aaagcgaatg gagatcactt tgggagctta ccgtgttcat
      961 accgtctgtt ttcaacagtt ttctccccgg gaagctaatc tgccataagc ctggataaca
     1021 gggcacggtg atactccgta atagcgatca gcacctccgc atactccctg tccccgatac
     1081 gcttgccgtc gataaagagt tttttctgta acgcccccag tctcataact tctttcagca
     1141 cccgatcatc agtcagtgag ttaacgttct taccgagagc tgccgcccgt aaaaaaccag
     1201 aaacggtctt gccgcattca gcagcccttt ttctgatttc ctggtcttct tctgccgtca
     1261 gacgaacagg gcgactgata gcccgcctgc gcgtattact tccacttcgt tttgtcataa
     1321 aacatggctc cgtatctgac acggggtgtc ggggcgaagc cctgaccagg gtaattgtaa
     1381 tagcgtgcat gtatgcgcgg tataacaatt acacatcctg tcctgtcagc aagttcgaat
     1441 taccgacaaa ggcacggcag tttttttggc agtgacagta ttgtcaggca gaaatacccg
     1501 tcaatgcgtc agacggcaga ataccggcgg taaaacagtc gaaaagtggt acgcgatata
     1561 atcacacaac gcagatgaca gacagaacgg agaaaacaga aagatgaaca agcagcagca
     1621 aaccgtactc aacatggcgg gattcataaa aagccagagc ctgacgctgc tcgaaaagct
     1681 ggacgcactc gatgccgacg aacaggccgc catgtgtgag aagctgcacg aaatcgcgga
     1741 agaactccag aacagcatac agacacgctt tgaagcggaa agtggcaccg gggtataaca
     1801 gtgtatcctg ctccagacag gatgcagaac ggtataccgg tgcgtaagga gaaaatgtca
     1861 cataaggcgc tcttccgctt cctcgctcac tgactcgcta cgctcggtcg ttcgactgcg
     1921 gcgagcggtg tcagctcact caaaggcggt aatgcggtta tccacagaat caggggataa
     1981 cgccggaaag aacatgtgag caaaaagccg aaggcaggga caaaggccgc gcaggtggcg
     2041 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     2101 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     2161 cgctctcctg ttccgaccct gccgtttacc ggatacctgt ccgcctttct cccttcggga
     2221 agcgtggcgc tttctcatag ctcacgctgt tggtatctcg gcccggtgta ggtcgttcgc
     2281 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     2341 aactatcgtc ttgagtccaa cccggtaaga cacgaattat cgccactggc agcagccact
     2401 gataaccgaa ttagaggagt aagacttgaa gtcatgcgcc tgttaaggct aaactgaaag
     2461 aacaagtttt ggtgactgcg ctcctccaag ccagttacct cggctcaaag agttggtagc
     2521 tctgagaacc ttcgaaaaac cgccttgcag ggcggttttt tcgtttacag gcagcagatt
     2581 acgcgcagaa aaaaaggatc tcaagaagaa cctttaattt tttttcacac ttggtatcct
     2641 actttaaccc ataaaataaa cacttacacc gacacaccct taaacaaaag
//
LOCUS       DAJTZL010000313         2616 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.316, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000313 DAJTZL010000000
VERSION     DAJTZL010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2616
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.316"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..1818
                     /locus_tag="OO544_004195"
     CDS             <1..1818
                     /locus_tag="OO544_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HCR6769639.1"
                     /translation="NYYLPKESAYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARV
                     GYSKMLLGVYAYFIEHKQRNTLIWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYGK
                     KHRDNTLTMKRFTNGRGFWCLGGKAAKNYREKSVDVAGYDELAAFDEDIEQEGSPTFL
                     GDKRIEGSVWPKSIRGSTPKVRGTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDK
                     ETPFGLKWTPDDPSSVFYLCEHNACVIRQQELDFTDARYICEKTGIWTRDGILWFSSS
                     GEEIEPPDSVTFHIWTAYSPFTTWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIG
                     ERPDAEVMAERKEHYSAPVPDRVAYLTAGIDSQLDRYEMRVWGWGPGEESWLIDRQII
                     MGRHDDEQTLLRVDEAINKTYTRRNGAEMSVSRICWDTGGIDPTIVYERSKKHGLFRV
                     IPIKGASVYGKPVASMPRKRNKNGVYLTEIGTDTAKEQIYNRFTLTPEGDEPLPGAVH
                     FPNNPDIFDLTEAQQLTAEEQVEKWVDGRKKILWDSKKRRNEALDCFVYALAALRISI
                     SRWQLDLSALLASLQEEDGAATNKKTLADYARALSGEDE"
     gene            1815..2021
                     /locus_tag="OO544_004196"
     CDS             1815..2021
                     /locus_tag="OO544_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family protein"
                     /protein_id="HCR6769640.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTATSVSDLK
                     KYIAELEVQTGMTQRRRGPAGFYV"
     gene            2018..>2616
                     /locus_tag="OO544_004197"
     CDS             2018..>2616
                     /locus_tag="OO544_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCR6769641.1"
                     /translation="MKTPTIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRAWNPPGES
                     VDAALLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFFRLSHRPSWRYLGIGEE
                     DARAFSREVEAAWKEFAEDDCCCIDVERKRTFTMMIREGVAMHAFNGELFVQATWDTS
                     SSRLFRTQFRMVSPKRISNPNNTGDSRNCRAGVQINDSGA"
BASE COUNT          625 a          670 c          781 g          540 t
ORIGIN      
        1 aattactatc tcccgaaaga atccgcatac caggaagggc gctgggaaac actgcccttt
       61 cagcgggcca tcatgaatgc gatgggcagt gactacatcc gcgaggtgaa tgtggtgaag
      121 tctgcccgtg ttggttattc caaaatgctg ctgggtgttt atgcctactt catagagcat
      181 aagcagcgca acacccttat ctggttgccg acggatggtg atgccgagaa ctttatgaaa
      241 acccacgttg aaccgactat tcgtgatatt ccgtcgctgc tggcgctggc cccgtggtat
      301 ggcaaaaagc accgggataa cacgctcacc atgaagcgtt tcaccaatgg gcgtggcttc
      361 tggtgcctgg gcggtaaagc ggcaaaaaac taccgtgaaa agtcagtgga tgtggcgggt
      421 tatgatgaac ttgctgcctt tgatgaggat attgaacagg aaggctctcc gacgttcctg
      481 ggcgataagc gtattgaagg ctcggtctgg ccaaagtcca tccgtggctc cacgcccaaa
      541 gtgagaggca cctgccagat tgagcgtgca gccagtgaat ccccgcattt tatgcgtttt
      601 catgttgcct gcccgcactg cggggaggag cagtacctta aatttggcga taaagagacg
      661 ccgtttggcc tcaaatggac gccggatgat ccctccagcg tgttttatct ctgcgaacat
      721 aatgcctgcg tcatccgcca gcaggagctg gacttcactg atgcccgtta tatctgcgaa
      781 aagaccggga tctggacccg tgatggcatt ctctggtttt cgtcatccgg tgaggagatt
      841 gagccgccgg acagtgtgac ctttcacatc tggacggcgt acagcccgtt caccacctgg
      901 gtgcagattg tcaaagactg gatgaaaaca aaaggggata cgggaaaacg taaaaccttc
      961 gtgaacacca cgctcggtga gacgtgggaa gcgaaaatcg gcgaacgtcc ggatgctgaa
     1021 gtgatggcag agcggaaaga gcattattca gcgcccgttc ctgaccgtgt ggcttacctg
     1081 accgccggta tcgactccca gctggaccgc tacgaaatgc gcgtatgggg atgggggccg
     1141 ggtgaggaaa gctggctgat tgaccggcag attattatgg gccgccacga cgatgaacag
     1201 acgctgctgc gtgtggatga ggccatcaat aaaacctata cccgccggaa tggtgcagaa
     1261 atgtcggtat cccgtatctg ctgggatact ggcgggattg acccgaccat cgtgtatgaa
     1321 cgctcgaaaa aacatgggct gttccgggtg atccccatta aaggggcatc cgtctacgga
     1381 aagccggtgg ccagcatgcc acgtaagcga aacaaaaacg gggtttacct taccgaaatc
     1441 ggtacggata ccgcgaaaga gcagatttat aaccgcttca cactgacgcc ggaaggggat
     1501 gaaccgcttc ccggtgccgt tcacttcccg aataacccgg atatttttga tctgaccgaa
     1561 gcgcagcagc tgactgctga agagcaggtc gaaaaatggg tggatggcag gaaaaaaata
     1621 ctgtgggaca gcaaaaagcg acgcaatgag gcgctcgact gcttcgttta tgcgctggcg
     1681 gcgctgcgca tcagtatttc ccgctggcag ctggatctca gtgcactgct ggcgagcctg
     1741 caggaagagg atggtgcagc aaccaacaag aaaacactgg cagattacgc ccgtgcctta
     1801 tccggagagg atgaatgacg cgacaggaag aacttgccgc tgcccgtgcg gcactgcatg
     1861 acctgatgac aggtaaacgg gtggcaacgg tacagaaaga cggacggcga gtggagttta
     1921 cggccacttc cgtgtctgac ctgaaaaaat acattgcgga gctggaggtg cagaccggca
     1981 tgacacagcg acgcagggga cctgcaggat tttatgtatg aaaacgccca ccattcccac
     2041 ccttctggga ccggacggca tgacatcgct gcgtgaatat gccggttatc acggcggtgg
     2101 cagcggattt ggtgggcagt tgcgggcgtg gaacccaccg ggtgaaagtg tggatgcagc
     2161 cctgctgccc aactttaccc gtggcaatgc ccgcgcagac gatctggtac gcaataacgg
     2221 ctatgctgcc aacgccatcc agctgcatca ggatcatatc gtcgggtctt ttttccggct
     2281 cagtcatcgc ccaagctggc gctatctggg catcggggag gaagacgccc gtgccttttc
     2341 ccgcgaggtt gaagcggcat ggaaagagtt tgccgaggat gactgctgct gcattgacgt
     2401 tgagcgaaaa cgcacgttta ccatgatgat tcgggaaggt gtggccatgc acgcctttaa
     2461 cggtgaactg ttcgttcagg ccacctggga taccagttcg tcgcggcttt tccggacaca
     2521 gttccggatg gtcagcccga agcgcatcag caacccgaac aataccggcg acagccggaa
     2581 ctgccgtgcc ggtgtgcaga ttaatgacag cggtgc
//
LOCUS       DAJTZL010000314         2586 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.317, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000314 DAJTZL010000000
VERSION     DAJTZL010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.317"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            196..1149
                     /locus_tag="OO544_004198"
     CDS             196..1149
                     /locus_tag="OO544_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6769642.1"
                     /translation="MKHELSSMKAFVILAESSSFNNAAKLLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKAGKRLLPEARELIKKFDETLFNIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDFGINMNNVTNSSIDFT
                     PLVNEPFVLACRRDHPLAKKQLVEWQELVGYKMIGVRSSSGNRLLIEQQLADKPWKLD
                     WFYEVRHLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIRRKD
                     AVLSPAAERFFALLINLWTDDKDNLWTNIVERQRHALQEIG"
     gene            complement(1190..2185)
                     /gene="pgl"
                     /locus_tag="OO544_004199"
     CDS             complement(1190..2185)
                     /gene="pgl"
                     /locus_tag="OO544_004199"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HCR6769643.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTCWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDHSGKYLIVAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(2445..2576)
                     /locus_tag="OO544_004200"
     CDS             complement(2445..2576)
                     /locus_tag="OO544_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001344347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA invertase"
                     /protein_id="HCR6769644.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNAFICAGSELIVERMGSWPEVVMA"
BASE COUNT          715 a          635 c          577 g          659 t
ORIGIN      
        1 tatttttttc ataataatgc cctcaattta aaaacataac ttattgcgat atggttacat
       61 taagggcaaa gcatctctaa tgactttagc tcaacgatta ataacatttt ttcattaatt
      121 taagttggct aaatgcagtt aaaattttta acggccagcc acccaaaatc gtcgcttcca
      181 ttttccggag taataatgaa gcatgaatta tcaagtatga aggcatttgt catactggca
      241 gagtccagtt cgtttaacaa tgccgctaaa ttactcaata ttacgcaacc tgcattaacg
      301 cgaagaataa aaaagatgga ggaagattta catattcaac tatttgagcg tacaactcgc
      361 aaggttacgt taacaaaagc aggaaaaagg ttgctcccgg aggcgcggga attaataaag
      421 aaattcgatg aaacgctttt taatattcgt gatatgaatg cttatcatcg tggtatggtg
      481 acgttagcgt gtattccgac cgcagtattt tattttttac cgctggcaat cggtaaattt
      541 aatgagctgt atcccaatat taaagtgcgg attctggaac aaggcacgaa taattgcatg
      601 gaatcggtgc tgtgtaacga atcagacttt ggcataaaca tgaacaacgt cacaaattca
      661 tccattgatt ttaccccact ggtcaacgag ccgttcgtgc tggcctgtcg gcgtgaccat
      721 ccattggcca aaaagcagct tgtagaatgg caggagttag tgggttacaa aatgattggc
      781 gtacgctcct ccagcggcaa ccgactgttg atagaacaac agctggccga caagccctgg
      841 aagctggact ggttttacga agtgcgtcat ctttcgacgt cattaggact ggtagaggcg
      901 ggactgggga tttcggcgct tcctggtctg gcaatgcccc atgcgcccta ttcttcgatt
      961 attggtattc cactggtgga acccgttata cgtcggacat tggggattat tcgccgcaaa
     1021 gatgccgtgc tttctccggc agcagaacgc ttttttgcct tgctgattaa tttgtggact
     1081 gacgataaag acaatttgtg gaccaatatc gtcgaacgcc agagacatgc gttacaggag
     1141 ataggttaat acaccggcgc aggagagact gcgccgggta aatcagcggt tagtgtgcgt
     1201 taaccaccac ccacattggt ccctgcccga ccgcatagcg gcctttttca tgcagtagcc
     1261 cctgctcgcc aacaatttcg tataccgaga tgtggtgaga tttttgcccg gcgacaatca
     1321 gatacttgcc gctgtgatca acattgaagc cgcgcggctg ggtttccgtt ggctggaagc
     1381 cttctttact caacacgctg ccatcttccg aaacgctgaa aacggtaatc agactggcgg
     1441 tacggtcgca ggcgtataaa tgacgaccat ccggggtgat atgaatatca gccgcccaac
     1501 aggtgtcgga gaagttttcc ggcatcatat ccagcgtctg gacacattca atattgccgt
     1561 gtggatcttt cagttcccag acatccactg agctgtttaa ctcattgacg caatacgcat
     1621 attgttcgtt tggatggaat accatatgac gcgggccggc cccttcaacg gtagtcactt
     1681 ccgcagggtc ctgcgccacc agatgaccat catcgctgac cgtaaacagg caaatgcgat
     1741 cctgctttaa tgccggaacc cacagcgtac ggttgtccgg tgagatattg gcggaatggc
     1801 aaccgtccag cccctcgacc acatcgacga cgcccactgg caggccatct tccagacgcg
     1861 ttacgctcac gttacccgca ttgtaagaac ctacaaagac aaactgcccc tggtgatcgg
     1921 tggaaatatg cgtcggacta cccggcagcg cagactctgc ggcaaaggtc agtgcgccat
     1981 cgtccggggc aatacgatac gccaggacgc gaaactcagg gcgaacacca acatagagat
     2041 aacgtttgtc cgggctgacc accatcggct gcacctgccc cggcacatcg acaacctgtg
     2101 tcagcgtcag tgcgccttca tgattcaggt tccagacgtg aatttgctgg ctctcagggc
     2161 tggcgatata aactgtttgc ttcatgaaca cacctcaaaa atgataaatc ccttatcttt
     2221 catacacata acaacactta ctggataaaa aacagacaac aaaacacgat aaaatacaat
     2281 atcgcacatc aattaaatca ataagttagc actccatttc gaatggcata ttactaaaat
     2341 attagtcacc gacacgaatt tgacaatgcc acagatattt tcgcgactat tcagggagta
     2401 tgactctccc cgcttttgtt tacctgtatc aacgaccgta aacactatgc cataacaact
     2461 tctggccaac ttcccattcg ctcaacaatc aactcacttc cggcacaaat aaacgcatta
     2521 cgttgtagat cggtattctg gtcatttgtt gatacgcgta catagccaat aagcatggta
     2581 aatccc
//
LOCUS       DAJTZL010000315         2573 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.318, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000315 DAJTZL010000000
VERSION     DAJTZL010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2573
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.318"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..2412
                     /locus_tag="OO544_004201"
                     /pseudo
     CDS             1..2412
                     /locus_tag="OO544_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001475310.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter barrel domain-containing
                     lipoprotein"
     gene            2414..2573
                     /locus_tag="OO544_004202"
                     /pseudo
     CDS             2414..2573
                     /locus_tag="OO544_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368382.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT          699 a          534 c          729 g          611 t
ORIGIN      
        1 attttaggat atcaagcgaa cagtaatggt aaggttatcg ttgaaaaggg tggcgagtgg
       61 ctaataaaaa ataatgattc ctcaattgaa tttcaaattg gtaatcaagg agctggggag
      121 gcgactattc gcgagggtgg gctgattacg gctgaaaata cgattatcgg tggcaatgcc
      181 accggtatcg gaaccctgaa tgtgcaggat caagactctg tcatcacggt acgcagactc
      241 tataatgggt atttcggtaa tggcacagtc aatatttcca ataatggtct gattaataac
      301 aaagaatatt cattggtggg cgttcaggac ggttcccacg gtgtcgtcaa cgtgaccgat
      361 aaagggcatt ggagtttcct cggaacaggc gaagctttcc gctatatcta tatcggtgat
      421 gctggtgacg gtgaacttaa tgtctcgcgt gaaggtaaag tagattcggg aattatcact
      481 gcggggatga aagaaacagg cacaggcaac attactgtta aggataagaa ctccgttatc
      541 actaatctcg gaactaatct tggttatgac ggccacggcg aaatgaatat cagtaatgag
      601 gggcttgttg tcagcaacgg aggaagctca ctcggttatg gagaaaacgg cgtcgggaat
      661 gtcagcatca ccacgggggg gatgtgggag gtcaataaga atgtctatac caccattggt
      721 gttgcgggcg tcggaaacct caatattagc gatggcggta agtttgtatc gcaaaatatt
      781 acttttttgg gcgataaagc aagcggtatc ggcacactga acctgatgga tgcgacatca
      841 tcgttcgata ctgtgggtat caatgtcggt aattttggta gcggtatcgt aaatgtcagt
      901 aatggtgcca cccttaattc aacgggctat ggatttatcg gaggaaatgc ctccggtaag
      961 gggatagtta atatttcaac ggacagtctc tggaatttaa agacatcatc tactaacgca
     1021 caattgctac aggtcggtgt attaggcacg ggtgaactga atattaccac cggaggtata
     1081 gttaaagcgc gtgatacaca gatagctctc aatgacaaaa gtaagggcga cgtgagggtg
     1141 gatgggcaga actctcttct tgaaacattc aatatgtacg tagggacatc tggtacgggt
     1201 acgttaaccc tgacgaatag cggcacgctg aatgtcgaag gtggagaagt ttacttaggt
     1261 gtttttgaac ctgctgtagg aacgctaaac attggtgcag ctcacggtga ggcggcggca
     1321 gatgccgggt atataaccaa cgcgacgaaa gtagagtttg gttctggcga aggtgttttt
     1381 gtctttaatc atactaataa cagtgatgcc ggctaccagg tcgatatgct gattacaggt
     1441 gacgataaag acggaaaagt gatccatgat gcaggccata cggtgttcaa tgcagggaat
     1501 acttatagcg gtaaaacgct ggtcaatgac ggcctcctga ccattgcgtc tcatacggca
     1561 gatggggtaa cgggcatggg ttcgagtgaa gtcaccattg caagccccgg tacgctcgac
     1621 attctcgcat caacgaacag tgcaggagat tacacgctga ccaatgcgct caaaggcgat
     1681 ggcttgatgc gagtgcagct gtcatcctac gacaagatgt ttggctttac ccatgcaaca
     1741 gggactgaat tcgccggtgt tgcccaactg aaagacagaa ccttcactct ggaacgcgac
     1801 aacaccgctg cgcttactca cgcgatgttg cagtctgaca gtgaaaatac cacatcggta
     1861 aacgtgggag agcaatccat tggtggactg gccatgaatg gcggtacgct cattttcgat
     1921 acggatattc ctgctgcgac gcttgcagag ggatatatca gcgtcgatac gctggttgtc
     1981 ggcgcgggtg actacacctg gaaaggccgt aactatcagg taaacgggac gggcgacgtg
     2041 cttatcgacg tgcctaaacc gtggaatgat cccatagcga ataaccctct gacgacgctc
     2101 aatttgctgg aacacgatga tagccatgtc ggcgttcaac tggtgaaggc gcaaacggtt
     2161 attgggtcgg gtggctcatt aacgttacgt gatttacagg gcgacgaggt ggaagcggac
     2221 aaaacgttac acattgcgca aaacggaacg gtggtcgccg agggtgatta tggattccgc
     2281 ctcacgaccg caccaggtga tggtttgtac gttaactatg ggctgaaagc gctgaacatc
     2341 catggtgggc aaaagctgac gttagccgaa catggcggag cctatggcgc aacggccgat
     2401 atgtcggcaa aatagccatc gcttacacga ccgtctgggt gagccgcagt atacagattc
     2461 actgcattct cagggatcgg caagcagtat gtggatgcgt catgtcgggg ggcacgaacg
     2521 tttcaggacc ggtgacggcc agctaaatac tcaggctaac cgctatgtat tgc
//
LOCUS       DAJTZL010000316         2556 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.319, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000316 DAJTZL010000000
VERSION     DAJTZL010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2556
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.319"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(58..261)
                     /gene="tatE"
                     /locus_tag="OO544_004203"
     CDS             complement(58..261)
                     /gene="tatE"
                     /locus_tag="OO544_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HCR6769645.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(390..1178)
                     /locus_tag="OO544_004204"
     CDS             complement(390..1178)
                     /locus_tag="OO544_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HCR6769646.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRRVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
     gene            1271..1654
                     /gene="crcB"
                     /locus_tag="OO544_004205"
     CDS             1271..1654
                     /gene="crcB"
                     /locus_tag="OO544_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HCR6769647.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(1708..1917)
                     /gene="cspE"
                     /locus_tag="OO544_004206"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(1708..1917)
                     /gene="cspE"
                     /locus_tag="OO544_004206"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HCR6769648.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(2092..>2556)
                     /gene="pagP"
                     /locus_tag="OO544_004207"
     CDS             complement(2092..>2556)
                     /gene="pagP"
                     /locus_tag="OO544_004207"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HCR6769649.1"
                     /translation="TFRENIAQTWQQPEHYDLYIPAITWHARFAYDKEKTDRYNERPW
                     GGGFGQSRWDEKGNWHGLYAMAFKDSWNKWEPIAGYGWESTWRPLADENFHLGLGFTA
                     GVTARDNWNYIPLPVLLPLASVGYGPVTFQMTYIPGTYNNGNVYFAWMRFQF"
BASE COUNT          652 a          659 c          580 g          665 t
ORIGIN      
        1 tttgaagcgg tttttttgta tcgaacaaga tattgaggga gcgtcctgct cgccacgtca
       61 ctctttatga gagagctttt cagcctgaag atcaacgtct gcgccttttt tcgccgcagc
      121 atcgtcatca ttcatcgcct tcttgaaccc tttaatggcc gctccaaggt ctccgcccag
      181 cgtacgtaac ttcttagtcc caaacagcag aacgaccagc gccgcaacta ccagcagttt
      241 ggtaatacta atctccccca tagatacctt cttgacatat aaccggcaac agaacgcgcc
      301 ttaaaaacca gagaatatta acggtcattc gacgcgcgca cacaatagca atctgtaaca
      361 aggtgaatca agcgccgagt aaaaaaacat cataataatt gcggcggcgc aaagcgacgg
      421 ttgtttaaga cgggcagttg cgcgcgcacc tgacgcacac gttcgggcgt cacttccgcc
      481 ataatgagtg caggcatttc tgacgctgcc gcaatggtga cgccaaaggg atctataatc
      541 cggctttgcc cgatattttt gttcccgcac tcccccgccg ccaccatata acaggtggta
      601 tccagcgcac gagcggcaag caacgttgac cagtgatgct ctttgagcgg cccgcgaacc
      661 caggcggcag gaagtaccag gatttcagct ccctgtaatg cctgtgccag cgccagctct
      721 ggaaagcgta agtcataaca ggtcatcaga ccgaccttca tcccctccac ctcgagtaac
      781 ggagcgattt cattaccagc atcaacacgg cgtgattcct gaatggcaaa tgcatcatag
      841 agatgcaatt tggcataacg ggcgacgatg tttcctgcct gtagcgccac cagcatattc
      901 catgcgcgcc ccggcgttga aggaacatga atcgtcagaa ttgtcgtcat catgttacgt
      961 ttactttctc gccgtaaacg tccgaggaat tcgccttcca gcagctgtgc tgatttaacc
     1021 gatagatctg catcatgatc atcgcgcgcc agcaatgctt ccggcaggac gaacagcgat
     1081 acgtcgtttt ccgccgcctg cgccatcaac gaggcacaaa tctcagcgtt cttttcccac
     1141 acagatgtaa cagcaaactg tcctgcggca accagcattg ccacctcctg tcagagtcgt
     1201 aaaattcgcc aacgacgatt attcggcgtt acacttatca ctcatacaaa tcaaatagca
     1261 ggattttgca gtgttacaac ttcttttagc agtttttatt ggcggtggta cgggaagcgt
     1321 ggcgagatgg ctgttaagta tgcgatttaa cccgctgcat caggcgattc cgttggggac
     1381 gctggcagca aacctgattg gggcattcat cataggaatg ggattcgcct ggttcagcag
     1441 gatgacgaac attgatccag tgtggaaagt attaatcacc accggatttt gtggcggtct
     1501 aacaaccttc tcaacatttt cggcagaagt ggtgtttttg ttacaagagg gccgctttgg
     1561 ctgggcgtta ctgaacgttt tcgtcaacct tctggggtct tttgccatga ccgcactggc
     1621 tttctggctg ttttcggcct caaccgcaca ctaaagacca aaaaaaaccc gctgattaag
     1681 cgggttttga attcttgctg acgtatctta cagagcgatt acgtttgcag cagaagggcc
     1741 tttggcaccg ttagtgattt cgaactctac gcgctgacct tcagcaagag ttttaaaacc
     1801 attagtctgt attgcagaga agtgtacgaa cacgtctttg ctgccgtctt ccggagtaat
     1861 gaaaccgaat cctttggact cattaaacca cttaacgtta cctttaatct tagacatcaa
     1921 aattaccttt acatgaaaaa tagacacaaa tgctgtgtcg gttaccagta caccaattgt
     1981 ggtacgcttt gtccagtgca actttactaa aaagtgataa aagtcgcgtt atttttgatg
     2041 ttacttattt tagttattga ttttaaagaa gttactaaaa cttcatttgt ctcaaaactg
     2101 aaagcgcatc caggcaaagt acacattgcc attgttgtag gtacccggaa tgtaggtcat
     2161 ctgaaaagtc actgggccat aacccacgga ggccaatggc agtagaaccg ggagagggat
     2221 gtaattccag ttatcgcgtg ccgttacgcc agcggtgaat cccagaccta aatgaaaatt
     2281 ttcatccgcc agcggtcgcc aggtactttc ccatccgtat ccggcaatcg gttcccattt
     2341 gttccacgag tccttaaatg ccatggcata caggccatgc cagtttcctt tttcatccca
     2401 tcgcgactga ccaaaaccgc caccccacgg tcgctcgtta tagcgatcgg ttttttcttt
     2461 gtcgtaagcg aaacgtgcat gccaggtgat ggcaggaata tataaatcat aatgttcagg
     2521 ctgttgccag gtttgtgcaa tattttctct aaacgt
//
LOCUS       DAJTZL010000317         2530 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.320, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000317 DAJTZL010000000
VERSION     DAJTZL010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2530
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.320"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(4..78)
                     /locus_tag="OO544_004208"
     tRNA            complement(4..78)
                     /locus_tag="OO544_004208"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(44..46),aa:Gln,seq:ttg)
     gene            complement(102..186)
                     /locus_tag="OO544_004209"
     tRNA            complement(102..186)
                     /locus_tag="OO544_004209"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(150..152),aa:Leu,seq:tag)
     gene            complement(195..271)
                     /locus_tag="OO544_004210"
     tRNA            complement(195..271)
                     /locus_tag="OO544_004210"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(235..237),aa:Met,seq:cat)
     gene            complement(651..2315)
                     /gene="asnB"
                     /locus_tag="OO544_004211"
     CDS             complement(651..2315)
                     /gene="asnB"
                     /locus_tag="OO544_004211"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HCR6769650.1"
                     /translation="MCSIFGVFDIKTDAVEQRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
BASE COUNT          609 a          638 c          627 g          656 t
ORIGIN      
        1 cgatggctgg ggtacctgga ttcgaaccag ggaatgccgg tatcaaaaac cggtgcctta
       61 ccgcttggcg ataccccatc cgtacaacgc tttctggtga atggtgcggg aggcgagact
      121 tgaactcgca caccttgcgg cgccagaacc taaatctggt gcgtctacca atttcgccac
      181 tcccgcaaaa aagatggtgg ctacgacggg attcgaacct gtgaccccat cattatgagt
      241 gatgtgctct aaccaactga gctacgtagc catctttttt tcgcgatacc ttatcggcgt
      301 tgcggggcgc attatgcgta tagagccttg cagcgtcaac ctctttttca aggaaaattg
      361 ctcgaaagtg actgtttggt taggttgcga acagcgtggc gctatattcg tcaattattg
      421 tttactttgt gttttttccc caccctacag ccattctttt gtcatacagg atgaaattcg
      481 gaattcaaca atggtggtgg gaaattaatc tatgaaatac tggcctacag tgatgagttg
      541 tcaaacagtg atgtggcaaa cccggaacat ttccttactg catatcagaa tcaacaagca
      601 cctcaataac tgaaacagcc ccggatttca ccggggctgt ttcgcatttc ttacttatac
      661 gccgactggt gaacgccaac cgcacgacca gacggatcgt ccattttctt gaacgcctca
      721 tcccactcaa tcgctttagc ggaagaacac gcgacggacg ggccgcccgg cacgcactca
      781 gcggcgctcg gaagcgggaa cagttcttca aagatctccc ggtacagata cgcttctttt
      841 gaggttggcg tgttgtacgg gaagcggaag cgggcagttt ccagttgctg atcagaaacc
      901 tgctgcgccg ccacttcttt cagggtgtcg atccaactgt aaccgacgcc atcggagaac
      961 tgctctttct gccgccaggc cacgcttgcg ggcaggtatg actcaaaaca ttcacgcagg
     1021 atgtgttttt ccattttgcc gttaccgcac attttatcct gcgggttaat gcgcatcgcc
     1081 acatcaagga attttttgtc gaggaacgga acgcgtgctt ccacgcccca ggctgacatc
     1141 gctttgttgg cgcgcgcgca gtcatacata tgcagggcca gcagtttacg caccgtctct
     1201 tcatgcagtt ctttagcgtt gggcgcttta tggaagtaaa gataaccgcc aaacacttca
     1261 tcagaacctt caccggacag caccatttta atgcccatcg ccttgatctt acgcgacatt
     1321 aaatacatcg gtgttgaagc gcgaatagtg gtcacatcat aagtttcgat gtggtaaatc
     1381 acgtcgcgga tggcatccag accttcctgt acagtgaagt gaatttcgtg atgcaccgtg
     1441 cccagatggt ttgccacttc ctgggctgct ttaagatccg gtgaacccgg cagacctaca
     1501 gcaaaggagt gtaactgggg ccaccaggct tcagagcgtt cctgatcttc cacgcgacgg
     1561 gctgcgtatt tcttggtgat agcggaaata attgaggaat ccagaccacc agaaagcagc
     1621 acaccgtaag gcacatcaga catcagatgg cttttaacgg aatcttccag agcctgacgc
     1681 agctcgtttt tgtcggtcac gttatctttc actgcatcaa agtcgaacca gtcgcgatga
     1741 taataagaac ggatttcgcc gtcctggctc cacaaatagc tccccgccgg gaactcttta
     1801 atcgtgcggc aaactggcac cagcgatttc atttctgagg ccacatacag ctgaccgtgt
     1861 tcgtcatacc ccatatacag tgggatgatc cccagatggt cgcgaccaat caggtaggca
     1921 tctttttcgc tgtcgtacag ggcaaaggca aacatgccct gcaagtcgtc gagaaattcc
     1981 ggcccttttt cctgatacag cgcgaggatc acttcacagt cagaccctgt ctggaactga
     2041 taacgatcgc catattcagc gcgcaatgcc tggtggttgt agatttcacc gtttaccgcc
     2101 agtacgtggg ttttttgttg gttgtagaga ggttgcgccc ccgcgttaac gtcaacaatt
     2161 gacagacgtt cgtgagcgag aatggcgtta tcgctggcat aaataccgga ccagtccggg
     2221 ccacgatgac gcatcaggcg tgacagctcg agtgctttct tacgctgctc aactgcgtct
     2281 gttttgatat cgaatacgcc aaaaattgaa cacataacct tctccgttaa cctggtattt
     2341 gttgcttgtt gtgtttgctt gtttaaaaaa atgccgcaaa gcagcactgt gcgcaagcga
     2401 tttggcggtg aaaaaataaa aaacgtaatg gtgattgtcg attggtgaaa aagggtctgg
     2461 tgtgaggatg tatttattga tgaatcgata atttttagcg ggttttattg aatggttata
     2521 ttttacgggg
//
LOCUS       DAJTZL010000318         2472 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.321, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000318 DAJTZL010000000
VERSION     DAJTZL010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2472
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.321"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(1..713)
                     /locus_tag="OO544_004212"
                     /pseudo
     CDS             complement(1..713)
                     /locus_tag="OO544_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001462196.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like adhesin"
     gene            complement(766..1362)
                     /locus_tag="OO544_004213"
     CDS             complement(766..1362)
                     /locus_tag="OO544_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6769651.1"
                     /translation="MHPTQRKLMKRIILFLSLQFPITYPAIAGQDIDLVANVKNSTCK
                     SGISNQGNIDLGVVGVGYFSDNVTPESYQPGGKEFTVTVSDCALQGTGDVLNQLHIDF
                     RALSGVMAAGSRQIFANEVATGAKNVGVVLFSIQDPTNIFNVISSAGNSRSVYPVMTS
                     ALHNSSWKFYARMQKIDPALDVISGQVMSHILVDVYYE"
     gene            complement(1389..1991)
                     /gene="yadL"
                     /locus_tag="OO544_004214"
     CDS             complement(1389..1991)
                     /gene="yadL"
                     /locus_tag="OO544_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein YadL"
                     /protein_id="HCR6769652.1"
                     /translation="MTFKNLRYGLSSSVVLAASLFSVPSYAATDSIGLTVITTVEIGT
                     CTATLVNDSDQDISVVDFGDVYISEINAKTKVKTFKLKFKDCAGIPNKKAQIKLTKRA
                     TCEGTANDGAGLANGSTAADKASAVAVEVWSTETPATGSATQFSCVTPASQEVTISNA
                     ANAVVYYPMSARLVVEKNKTVSNVTAGKFSAPATFTVTYN"
     gene            complement(2006..>2472)
                     /locus_tag="OO544_004215"
     CDS             complement(2006..>2472)
                     /locus_tag="OO544_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6769653.1"
                     /translation="ATTCNITLTGNNVTNDGNNNYTLRIPKMGLDKIANKTTESQADF
                     KLVASGCSSGISWIDTTLTGNASSSSPKLIIPQSGDSSSTTSNIGMGFKKRTTDDATF
                     LKPNSAEKIRWSTDEMQPDKGLEMTVALRETDAGQGVPGNFRALATFNFIYQ"
BASE COUNT          680 a          537 c          495 g          760 t
ORIGIN      
        1 tggcctgcgc cataggcttt aaatgaatac agataatttg atgagctgga taatttgatc
       61 tgttgagtta ctgttggatc aaagtttgta tctgtataga attcaattgt aagggtctga
      121 taaataccac ctacagccca gtatttacgg gtgctattcg cattattgca actccaatat
      181 aacacgtctt cgcttggatt ataccacgaa aaatattgtt ctgcagagtc accaatgtat
      241 atcccaggtg aactaacgtt agttacggta ccgtaagctg accacatgtt tgaaattaat
      301 aatgtgtaat acaaaccagg aactgatgta ttaaataatt tatgggagcc atacatttta
      361 cctgagtcaa ccattgcatt ttcaatacgc attgcgctac cacttgtgct atcacttctg
      421 ttgcaataaa gaaaaccacc accagaagca gtatctaact gactctactg taattttaca
      481 cctgtaggtc caagctgcgt cggtttgtag aaaactaaat tatttgtact tgttgcatat
      541 ctgaatatga cagtagcatc gccattcggc cccgtttctc tccatttttg atctgacgga
      601 ccaacgccgt aactattccc aacaatatat cctacgttat catcagtgcc agcactcgcg
      661 gtataacaac tcaagcacag aattaaaaaa aaatgtctga agaatttctt cattatcact
      721 ccaccatcta ctgctgtaac agtcgcagtt atttgttttg atctattatt cgtagtacac
      781 atcaactaat atatgactca ttacttggcc agatatgaca tccagcgcgg gatcgatttt
      841 ttgcattctg gcataaaatt tccatgaaga attatgcagg gcagaagtca ttactggata
      901 cacggaacga gaatttcctg ctgaactaat aacattgaat atattggtcg ggtcctgaat
      961 ggaaaagaga acgacaccga catttttagc cccggttgcg acttcattgg caaatatttg
     1021 ccttgaacct gcagccatga caccgctaag ggctctaaaa tcaatatgta actgattaag
     1081 cacatcgcca gttccctgta atgcacagtc tgatactgtg acagtgaact cttttccacc
     1141 tggttgatag ctttcaggag taacattatc tgaaaaatat cccaccccaa cgacgccaag
     1201 atcaatatta ccctggttac tgattccgct tttgcaagtg ctgtttttta cattggcaac
     1261 aaggtcaata tcctgcccag cgatggctgg ataggtaatg ggaaactgta gtgacagaaa
     1321 cagaattatt ctcttcatca gcttacgttg agtgggatgc atatatagtt cctggatctg
     1381 tcagtctgtt agttataggt tactgtaaat gtggcaggtg cagaaaattt acccgcagtg
     1441 acattgctta cggttttatt tttttcgacg accagtcgag cactcatcgg ataataaaca
     1501 accgcgttgg ctgcattgga gatagtgacc tcttgagatg ctggtgtcac acaactaaat
     1561 tgtgttgcgc tccctgttgc cggagtttcg gtgctccaga cttcaacggc gacggcactt
     1621 gctttatctg cggctgtgga tccatttgcg agccccgcac cgtcattagc agtaccttcg
     1681 catgtggctc gcttggttaa ttttatttgt gcttttttat tggggatacc cgcacagtct
     1741 ttgaatttga gtttgaatgt tttaactttg gtcttggcat tgatttcaga aatatataca
     1801 tcgccaaagt caacaacaga aatgtcctga tcagagtcat ttactaatgt agcggtacaa
     1861 gtacctattt ctacggtagt aatcacggtc agcccaatgc tgtctgttgc cgcataagag
     1921 ggtacgctga acaatgaggc agccaaaacg acactgctgg ataatccata acgtaaattt
     1981 ttaaatgtca tcttaattat ccttcttatt gatagatgaa attaaacgtt gccagtgcac
     2041 gaaaattccc cggtacgcct tgccctgcat ctgtttcacg tagcgcaacg gtcatttcaa
     2101 gacccttatc gggctgcatc tcgtctgtgc tccagcgtat cttttccgca ctgttaggtt
     2161 taaggaaagt ggcatcatca gtagtccgtt ttttgaaacc cataccgata ttacttgtcg
     2221 tcgaagatga atcaccagac tgcggtataa taagcttagg tgagcttgat gatgcatttc
     2281 cggtcagagt ggtatcaatc caactgatgc cactgctgca cccactagca accagtttaa
     2341 aatcagcctg agattctgtc gttttattcg cgatcttatc cagacccatc ttagggattc
     2401 tcaatgtgta gttattattg ccatcattcg tgacgttatt accagtaagt gtgatgttac
     2461 aggttgtcgc tt
//
LOCUS       DAJTZL010000319         2464 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.322, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000319 DAJTZL010000000
VERSION     DAJTZL010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2464
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.322"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            385..>2464
                     /locus_tag="OO544_004216"
     CDS             385..>2464
                     /locus_tag="OO544_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6769654.1"
                     /translation="MTNKKNLSNPFSTGGGGVHFEAHVQASFVALMLTGGNAPCLPCW
                     PIVEINLQGKIDGFDTDDLIVVVKDFNSNEKRKLLGQIKHSISFTQGNAILGEVIQAA
                     WNDFNNPDLFTKNKDVIALITGPLSSTDTHNVLWLLNQARHTKTVDEFLRNVQKAKFS
                     PPKSTEKLEAIQFHLKMANSGNDLTNDELYDFLNHFHLLGYDLGNESGVVLSLLHSHI
                     AQFHQQYPQWVWSRIVDIVQTWNKDAGTIISSKLPEDLLDVFKQRVIAEIPEKLRLVQ
                     PVVKTDWTHHPDASYLALAVLIGQWNDKNECDRNVLTQLLGISYDEWLRKAREILHSP
                     DSPLSLKNGIWKVTNRMELWSLLGSRIFDQNLELFKSSAISVLKEPDPAFELPAEERY
                     AASIHGKVLKFSSTLRQGIAEGLAIIGSRPEVCVNCSQGNAETSVNVAIHTILTDANW
                     VLWGSLNGLLPTLAEAAPAKFLDAVEKAMRQTPCVFDELFSQEVNGITGGNYLTGLLW
                     ALEELAWDEQYLVRVCVVLAELASHDPGGKWANRPSNSLTTILLPWFPQTLASIEKRK
                     VAVNVILQEWPDIAWNLLVQLLPGHHQTSSGSHKPSWRRIIPDDWESKVTNQDYWLQT
                     SFYAELAVKNAGEDIERLTLLIDNFDSLPSPAFEHLLKHLSSETINCLNEEQRVVIWD
                     HLHRFANKHRR"
BASE COUNT          708 a          510 c          541 g          705 t
ORIGIN      
        1 tgcatgacaa ggtcatcggg cattatctga acataaaaca ctatcagtaa gttggagtca
       61 ttaccgtact ctccttggag tatgaagacg ttactcactt taagattagc atcctcaaac
      121 cactgtgaat ttgctcccat acggaaagag gtgactcaga cctacaaaaa tttgtcaggc
      181 ccgaagggta tcttgcgcca gcataaaata ctggatataa cctaactatt cattttagca
      241 atagccattg cttacgagag ctagctaatc tacgtaaatg ctaggcataa cagtgtcttt
      301 aaaagatata ggtggaataa gttctgaagt atgagtgctc ttctttttca tctattcgta
      361 atcaaaatgt ggatgtgatt gttaatgaca aataaaaaaa atttgagtaa tcctttttct
      421 acaggcggag gcggtgttca ttttgaagca catgtccaag cgtcttttgt tgctctaatg
      481 ctaacaggcg gtaatgcacc ttgtcttcct tgttggccca tagtggagat aaatctccag
      541 ggcaagattg atggttttga taccgacgac ttgatcgtcg ttgttaaaga tttcaacagc
      601 aatgaaaaac ggaagctatt aggacaaata aagcattcta tttcatttac gcaaggtaat
      661 gcgatacttg gggaagttat acaggctgct tggaacgatt tcaacaatcc tgacttgttt
      721 accaagaaca aggatgttat tgcactgatc accggtccgc tcagttcgac cgatacacac
      781 aatgtgctct ggttattgaa tcaggctaga cacacaaaaa cagtcgatga atttttgagg
      841 aacgttcaaa aagccaagtt cagcccgcct aaaagtactg agaaactgga ggccatccag
      901 tttcatctga agatggcaaa cagtgggaac gatttaacaa acgatgagct ttatgacttt
      961 ttgaaccatt tccatttact agggtacgat ctgggtaatg aatcaggcgt cgtcctttct
     1021 ttgctgcact cccacatagc gcaattccat caacagtatc ctcagtgggt ttggtcgcgg
     1081 atagttgata tcgtacaaac ttggaataaa gatgctggca ctatcatttc aagtaagcta
     1141 cctgaagatc ttttagatgt tttcaaacaa agggttattg ctgaaatacc tgagaagttg
     1201 aggttagtac aaccggtagt caaaaccgac tggactcacc acccagatgc ctcttattta
     1261 gccttagcag tgttgattgg gcaatggaat gataaaaatg agtgtgaccg aaatgtacta
     1321 acccaattgc ttggtatcag ctatgacgaa tggctaagga aggctcgaga gattttacat
     1381 agcccagaca gtcctttgtc cctaaaaaac ggcatttgga aagtaacaaa tcgaatggag
     1441 ctatggagtc ttcttggatc ccgcattttt gatcagaatc ttgaattgtt taagtcttcc
     1501 gcgatttccg tactcaaaga gccagaccct gctttcgaat tacctgctga agagcgttac
     1561 gctgctagta ttcatggtaa ggtgctgaag ttttcatcca cgctgcgaca aggtatagca
     1621 gagggtttag ccataatcgg gagtcggcca gaggtttgcg ttaactgctc tcaggggaat
     1681 gctgaaactt ccgttaacgt cgcaatacat acaattttga ctgatgcaaa ctgggtttta
     1741 tggggtagcc taaacggttt gctaccaact ttggctgaag cagctcctgc aaaatttctt
     1801 gatgcagttg agaaggcaat gcgtcagact ccttgtgtat ttgatgagct tttttctcaa
     1861 gaagtaaatg gtatcactgg tggtaattat ctgacaggac tgctatgggc tctagaagaa
     1921 ttagcctggg atgagcaata cctagtacgc gtctgtgttg tactggccga acttgccagc
     1981 cacgatcctg ggggcaagtg ggctaatcga ccatctaact cgcttactac tatattatta
     2041 ccgtggtttc cgcaaacgct ggcatctata gaaaaacgaa aagttgcagt caacgtcatc
     2101 ttgcaggaat ggcctgacat tgcctggaat ttactcgtcc agcttctacc cggtcaccat
     2161 caaacctctt ctgggtcaca caagccctct tggcgccgaa taatcccaga tgattgggaa
     2221 agcaaagtga ctaatcagga ttactggttg cagacatctt tttatgctga gcttgccgtc
     2281 aaaaacgccg gagaagatat cgaacgcctt accttgttga ttgataattt tgatagcctt
     2341 ccatctcctg ctttcgaaca tcttcttaag cacctctctt cggaaacaat taattgtctt
     2401 aatgaagagc agcgagttgt aatttgggat catcttcatc gattcgcaaa taaacatcgg
     2461 cgat
//
LOCUS       DAJTZL010000320         2458 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.323, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000320 DAJTZL010000000
VERSION     DAJTZL010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2458
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.323"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(946..1104)
                     /locus_tag="OO544_004217"
     CDS             complement(946..1104)
                     /locus_tag="OO544_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6769655.1"
                     /translation="MKNIAAIKRNNRKIHARKFLSTPEGKAWLERKQRENEERKLLSE
                     LKWLREDF"
     gene            1991..2203
                     /locus_tag="OO544_004218"
                     /pseudo
     CDS             1991..2203
                     /locus_tag="OO544_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2245..>2458)
                     /locus_tag="OO544_004219"
     CDS             complement(2245..>2458)
                     /locus_tag="OO544_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6769656.1"
                     /translation="SFSKIILDERMQMAEYLLSTRCYPISKVAKVCGYASVSYFTYVF
                     RRYFGVSPSQYSQRSSESKILTHQGI"
BASE COUNT          770 a          517 c          450 g          721 t
ORIGIN      
        1 gcctggcata gcaagccctt ttcttgtgtt gtcaagacat ttggcaacgt tcaaacatct
       61 gtttagaaat gccaaattgt tgatctaaca aataaacctg tataaacaga acaccattta
      121 atctattccc ctgtgtaacc caccacatca cacaataaaa acaaaaaact ttaattatat
      181 ttcatagcac acaaaagtca atagaagccc tttcgtgctc ctacgtaaaa aatccatcac
      241 tttttccact gatacatcaa ttcaaggcgt tatatgtgtt ccttagcaca tataacattt
      301 attatgttaa atggattttg gcctatattt tgacctgcac ccgcataaac actggtttca
      361 tcgcttacag cccttgtgag acgtggtgct gtttctagca tctgatccga cataaaaccg
      421 atcattctcg tgtttagtga aagtttagtg agagacatta cagaaaggct ttctgtgtaa
      481 tgatttaata ggtaaaaacg atgttgatta cgcaagaaaa ttccttgctg atatttgggg
      541 attctggatt ttccagatcg gggggcgctg aaaatcgcgt taagcacctc tcgcaatcta
      601 gccataaggg aagggatata ggtattaaga tgtatttata gctatttatg gctatttatt
      661 ttttatttac ctttacttaa aaataacctt ataaatcaat aagatacata attatctgtt
      721 tatattttaa tcaattaaat caagtttaat acccttaaat cacctgaaaa tagccttaaa
      781 taccccaata attaatttta aggttttccg tgttctcttc cgcattatga aaatgcaaaa
      841 taaggcacaa aacaagcgca cagacgcatt aaacacacat cacctatccc tataccgagt
      901 aatgaaaaca gcgttagaat gcgtcctgtt gcgttctgga gattttcaga aatcctctct
      961 aagccatttc aactcactaa ggagttttct ttcttcgttc tctctctgtt tacgctctag
     1021 ccaggctttt ccttctggcg tagacaggaa cttacgagcg tgaatcttgc ggttattacg
     1081 tttgattgct gcaatgtttt tcatcgtgtg cctctgtatt catatgaaaa ttaacaagtt
     1141 acatactaca cagggagact acgcataaaa cacaatacaa gtaaagatac attttcaaga
     1201 caataggagc cataaagcgc ctttgatggt tataccaaaa aataagcgcc gtaggcgtat
     1261 ccactaaaaa gggctttttc acaaaagcta tgaagtctta gcagttcttc tcttatcagg
     1321 cacccgttgt actccggtgg acgaaaccgt gctagagctt gattctttcc cgatcaagtt
     1381 aggttttgaa gaggtgggct ttaatccact attgcactta aaactcttcc catcatttag
     1441 cctgtttatc ctctgcaacg tctcacgacg tgtagcatca ttggacaccg cgccaggtgt
     1501 ggaacatctt gctaagagtt ttggtagaag gtttagagac ggtggcacgt cttccagctt
     1561 caaaggaact tgttacgagg ttgagcctgg gaactactcg cccatacgtg ttacactacc
     1621 aaagcccata acaacgtata ggcgtttgta agtgataaca gaagatacaa tttcttagag
     1681 aagttaagct aagttattaa ttttcttaat ggtgccgata ataggagtcg aacctacgac
     1741 cttcgcatta cgaattataa gaatccgctt ctaattcaaa gcattacccc atcaacactg
     1801 cgctcacacg tcccaccaca tcaaaacatg taaagccttg caagccattg tgaggcctta
     1861 tgtgtctcag ttttgtccca ctacgacctg cacagaaaat aagaaaatag cggtgatatc
     1921 aaacagtaca gaagtctttt ttctttcaaa tggagaaact gattaacccg tttaattgta
     1981 ctcctcatgg gcaatcagca gtcctaagag taatggaatg gcagaaaggt tcgtgaaaac
     2041 gatgaaggaa gactacatcg cgttcatgcc gaaaccgaat gtaagaacgg cattgcataa
     2101 tcttgcagtg gcgatcgaac attacaatga aaaccatccg cacagtgcgt tgggttatcg
     2161 ctctccgcga gaatatcgac gtcagcgggt aacgttaact taagatacac cccctgtctg
     2221 gaaataaggg ggcaagaata atgatcagat tccctggtga gtaagaattt tactttctga
     2281 actcctctga gagtattgac ttggagaaac accaaaataa cgtctaaata cataagtaaa
     2341 gtatgagaca ctggcataac cacagacctt agctacttta ctaataggat aacaacgagt
     2401 gctgagtaaa tattcagcca tttgcatcct ctcatcaagt atgatcttac tgaatgat
//
LOCUS       DAJTZL010000321         2446 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.324, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000321 DAJTZL010000000
VERSION     DAJTZL010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.324"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(107..1525)
                     /gene="phrB"
                     /locus_tag="OO544_004220"
     CDS             complement(107..1525)
                     /gene="phrB"
                     /locus_tag="OO544_004220"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HCR6769657.1"
                     /translation="MTTHLVWFRQDLRLHDNLALAAACRNSSARVLALYIATPRQWAT
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVEVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIEPSPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCQN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGVGSVWLNEL
                     IWREFYRHLITYHPSLCKHRPIIAWTDRVQWQSNPAHLQAWQEGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPTTQGEKFDLEGEFIRQWLPELRNVPGKSVHEPWKWAQKAGVKLDYPQP
                     IVEHKEARVQTLAAYEAARKGK"
     gene            complement(1522..2031)
                     /locus_tag="OO544_004221"
     CDS             complement(1522..2031)
                     /locus_tag="OO544_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HCR6769658.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDYYNQYRQRVVVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYHDAWLSGQRYFELWPRV
                     INLRHSGVL"
BASE COUNT          622 a          630 c          559 g          635 t
ORIGIN      
        1 caggcctacg tatttcctgc aatttattga atttgcacaa atttgtaggc tggataaggc
       61 gttcacgccg catccggcat ctgggctcga ttgcctgacg cgtctgttat ttccccttcc
      121 gcgccgcctc atacgctgcc aacgtttgta ctctcgcctc tttgtgctcg actatcggtt
      181 gcggataatc cagcttcaca cctgctttct gcgcccactt ccacggctca tgcaccgatt
      241 tccccggtac attgcgtagt tccggtagcc actggcggat aaactcacct tcgagatcaa
      301 atttctcgcc ctgggttgtc gggttgaaaa tacgaaaata cggcgctgca tcggttccgg
      361 ttgaagcggc ccactgccag ccaccgttat tggctgccaa atcaccatca atcagctgcg
      421 acatgaaata tcgctcgcct tcgcgccagt cgatcaataa atctttcacc agaaaactgg
      481 ctgtaatcat ccgtagcctg ttatgcatcc agccagtgct gttaagctga cgcatagcgg
      541 catcaacaat cgggtatccc gttttgcctt cctgccaggc ctgtaaatgt gcgggattgc
      601 tctgccactg tacacgatcc gtccaggcaa taattggacg atgtttacac aacgaggggt
      661 gatacgttat caggtgacgg taaaactcgc gccagatcag ctcattaagc cagacactac
      721 cgaccccacc gtccagcgcc tgcggctgtt cagccaacaa gcgatgcaag cactggcgag
      781 gcgataaccc gcccgttgcc aggctggccg acaaacggct ggtgccttcc actgccggaa
      841 aatctcgttg ttgctcatat tctccggcac cgttctggca aaactggcgt aattgcgcaa
      901 tcgccgcttt ttcttccacc ggaaaatgcg cagtatcgaa agactgacga ggataattca
      961 gcgtaatgga tggcgagggc tctatcgatc cgctactacg aacttttggc gcagcgacgc
     1021 actccggcat cccttcccgc agccgtttca gccaggcatt cttaaaaggc gtaaagactt
     1081 tgtacatctc gtgattaccg gtcatcaccg cgccaggcgg caggatcacg ctgtcatcaa
     1141 atccttcaca caccacgtta cgcagcgctc tttcaacttc cacatcccgc gcccgctcat
     1201 tcacttcata ctgatagtta taaaacaggt gggtaacgct gttttccgcg cacacctgtt
     1261 taactatttc gacactggcg acaaagtcat ccacttcacg gaacaataaa ggaatacctt
     1321 tttccgcaag cgctatttgt agcccattca gttgagcatt gataagttca gcctgacgcg
     1381 gcgacatgtt atgcgtcgcc cactggcgtg gtgtagcgat atacaacgcc agcacgcgtg
     1441 cagacgaatt gcggcaggcg gcagccagtg cgagattatc gtgcagacgt aaatcttggc
     1501 gaaaccagac cagatgggta gtcataaaac tcctgaatgg cgcaagttaa tcacacgcgg
     1561 ccaaagttcg aaataacgct gccctgaaag ccaggcgtca tgataaagcg ccatatagtg
     1621 tttgatacgc atcagcggcg caagaagtgg ttgctcgcca cggcgataac tgtcgataag
     1681 cgcagccagc tgctggcgtt ctgtggaatc aatatgcggg cgaaaataac cctgaacgtg
     1741 catcaaaaca ttggtgtgat cgcgcgggtt ggcggggtga gaaagcaaaa caactacgcg
     1801 ttggcggtac tggttgtaat agtcatccag attttgccac tggtgaatat cagccacgaa
     1861 cgggccaagt ttgcggtact ccggctgaga atgcgccaga aagacgagtt tatagcggct
     1921 gtgataatcg agaagcgccc cgcgcgttaa gccatgttct ttcagtcgat gtaactcatg
     1981 aagggcaaag atacggatta gggtgctgtc atcaaatcgt tgtagattca ttactgctcc
     2041 tgaacggtat aaaggagtaa gtgtagacga tggtaaatga attttatggg gcagagtttt
     2101 tgcggggaag tattttgttg gtaaaaatat tatcaacttc atctgagtga gaattattct
     2161 actatttccc gagctgcatt cattttttgt attttttatt tttagaaaat aacagttctc
     2221 aaagcttaat tacctcacta ttaattgtat ttcaaactaa caatcgcact ccattttctc
     2281 tttttaaaaa acataaacac tgttaaaaca aatacttatt tcgttatctt aaatgctatt
     2341 tctacatcac tcgtccttat aatcaaatta ttcttattca tagctatttt tgcaggttta
     2401 aaatagcggc tttcaccaaa gtcagccatg aaaattagac tacaag
//
LOCUS       DAJTZL010000322         2426 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.325, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000322 DAJTZL010000000
VERSION     DAJTZL010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2426
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.325"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            284..805
                     /gene="fecI"
                     /locus_tag="OO544_004222"
     CDS             284..805
                     /gene="fecI"
                     /locus_tag="OO544_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FecI"
                     /protein_id="HCR6769659.1"
                     /translation="MSDRATTTASLTFESLYGTHHGWLKSWLTRKLQSAFDADDIAQD
                     TFLRVMVSETLSTIRDPRSFLCTIAKRVMVDLFRRNALEKAYLEMLALMPEGGAPSPE
                     ERESQLETLQLLDSMLDGLNGKTREAFLLSQLDGLTYSEIAHKLGVSISSVKKYVAKA
                     VEHCLLFRLEYGL"
     gene            802..1755
                     /gene="fecR"
                     /locus_tag="OO544_004223"
     CDS             802..1755
                     /gene="fecR"
                     /locus_tag="OO544_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fec operon regulator FecR"
                     /protein_id="HCR6769660.1"
                     /translation="MNPLLTDSRRQALRSASHWYAVLSGERVSPQQEARWQQWYEQDQ
                     DNQWAWQQVENLRNQLGGVPGDVASRALHDTRLTRRHVMKGLLLLLGAGGGWQLWQSE
                     TGEGLRADYRTAKGTVSRQQLEDGSLLTLNTQSAADMRFDAHQRTVRLWYGEIAITTA
                     KDALQRPFRVLTRQGQLTALGTEFTVRQQDNFTQLDVQQHAVEVLLASAPAQKRIVNA
                     GESLQFSASEFGAVKPLDDESTSWTKDILSFSDKPLGEVIATLTRYRNGVLRCDPAVA
                     GLRLSGTFPLKNTDAILNVIAQTLPVKIQSITRYWINISPL"
     gene            1842..>2426
                     /gene="fecA"
                     /locus_tag="OO544_004224"
     CDS             1842..>2426
                     /gene="fecA"
                     /locus_tag="OO544_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate transport protein FecA"
                     /protein_id="HCR6769661.1"
                     /translation="MTPLRVFRKTTPLVNAIRLSLLPLAGLSFSAFAAQVNIAPGSLD
                     KALNQYAAHSGFTLSVDASLTRGKQSNGLHGDYDVESGLQQLLDGSGLQVKPLGNNSW
                     TLEPAPAPKEDALTVVGDWLGDARENDVFEHAGARDVIRREDFAKTGATTMREVLNRI
                     PGVSAPENNGTGSHDLAMNFGIRGLNPRLASRSTV"
BASE COUNT          577 a          696 c          639 g          514 t
ORIGIN      
        1 cttaaaatca ccatcataca ctaaatcagt aagttgacag cattaccgaa atccctatcc
       61 tttagggcag tgaggatgtc aataatagca gaccacatca tgagtcattc tgaccgacac
      121 atgccagaag cagaggctcc atcccatttt atacctacct tataacactt agaaaaacaa
      181 catgttaaaa atgtctattg gaaacaattt tatttccaat tgtaatgata actattctca
      241 tattaatatg gctacgtgat aattaacttt tgatgcactc cgcatgtctg accgcgccac
      301 taccacagct tccttaacat tcgagtcgct ttatggcaca catcacggct ggttgaaaag
      361 ctggctgacg cgcaaactcc agtctgcttt tgatgcagat gacattgccc aggacacttt
      421 tttgcgggta atggtcagcg aaacgctctc gacgatccgc gatcctcgct ccttcctctg
      481 cactatcgcc aaacgcgtga tggtggacct gtttcgccga aacgcgctgg aaaaagcgta
      541 tctggagatg ctggcgctta tgccggaggg gggagcgcct tcacctgagg aacgcgaaag
      601 ccaactcgag accctacaac tcctcgacag catgctggac gggctaaacg gcaaaacacg
      661 tgaagcgttt ctgctttcgc aactggatgg tctgacatac agcgagattg cgcacaaact
      721 cggtgtttcc atcagctccg tgaaaaaata cgtggcgaaa gccgtcgagc actgcctgct
      781 gttccgtctg gagtatgggt tatgaatcct ttgttaaccg attcccgccg tcaggcgctg
      841 cgttcagctt cccactggta tgccgtgcta agcggcgagc gcgtcagccc acaacaggaa
      901 gcgcgctggc aacagtggta tgaacaggat caggataacc agtgggcctg gcagcaggtt
      961 gaaaacctgc gcaaccagct tggcggtgtg cctggcgacg ttgccagccg ggcgttgcac
     1021 gatacccgcc tcacccgccg tcacgtgatg aaaggattac tgctgttgct cggcgctggc
     1081 ggaggctggc agctctggca gtcggaaacc ggcgaaggtc tgcgggcaga ttaccgcacc
     1141 gccaaaggca cagtcagccg tcagcaactg gaagatggct ccctgctcac gctgaatacc
     1201 caaagcgcgg cggatatgcg ttttgatgcg catcagcgca ccgtccggct ctggtacggt
     1261 gaaatcgcca ttactaccgc gaaagatgcc ctgcaacgcc ccttccgtgt cctgacccgt
     1321 cagggccagc tcactgcttt agggacagaa tttaccgtcc gccagcagga taatttcacg
     1381 cagcttgacg tgcagcagca cgccgtggaa gtgcttctcg ccagtgcccc cgcgcaaaaa
     1441 cgcatcgtga acgctggtga aagcctgcag ttcagcgcct ctgagtttgg cgcagtgaaa
     1501 ccgctggatg acgagagtac aagctggacg aaggacatcc tgagcttcag cgataaaccg
     1561 ctgggtgagg tgatagccac gctaacccgt taccgcaacg gcgtgctgcg ctgcgatccc
     1621 gccgttgccg ggctgcgcct gagcgggacg ttcccgctga aaaataccga tgcgatcctg
     1681 aacgttatcg cgcaaacgct tcccgttaaa attcagtcta ttacgcggta ctggataaac
     1741 atttcaccac tgtaaggaaa ataattctta tttcgattgt cctttttacc cttctcgttc
     1801 gactcatagc tgaacacaac aaaaatgatg atggggaagg tatgacgccg ttacgcgttt
     1861 ttcgtaaaac aacacctttg gttaacgcca ttcgcctgag cctgctgccg ctggccggtc
     1921 tctcgttttc cgcttttgct gcacaggtta atatcgcacc gggatcgctc gataaagcgc
     1981 tcaatcagta tgccgcacac agcggattta ccctctcggt tgacgccagc ctgacgcgcg
     2041 gcaagcagag caacggcctg cacggcgatt acgacgtcga gagcggcctg caacaactgc
     2101 tggacggcag cggactgcag gtaaaaccgc tgggaaataa cagctggacg ctggagcccg
     2161 cgcccgcacc aaaagaagat gccctgaccg tggtcggcga ctggctgggt gatgcgcgtg
     2221 aaaacgacgt atttgaacat gctggcgcgc gtgacgtgat ccgccgtgag gatttcgcca
     2281 aaaccggcgc aaccaccatg cgtgaggtac ttaaccgcat ccctggcgtc agcgcgccgg
     2341 aaaacaacgg caccggcagc cacgacctgg cgatgaactt tggcatccgg ggcctgaacc
     2401 cgcgcctcgc cagccgctcg accgtc
//
LOCUS       DAJTZL010000323         2405 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.326, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000323 DAJTZL010000000
VERSION     DAJTZL010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.326"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            376..642
                     /gene="hipB"
                     /locus_tag="OO544_004225"
     CDS             376..642
                     /gene="hipB"
                     /locus_tag="OO544_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HipB"
                     /protein_id="HCR6769662.1"
                     /translation="MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISN
                     FENNPDNTTLTTFFKILQSLELSMTLCDAKNASPEATEQQDLEW"
     gene            642..1963
                     /gene="hipA"
                     /locus_tag="OO544_004226"
                     /pseudo
     CDS             642..1963
                     /gene="hipA"
                     /locus_tag="OO544_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416024.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system serine/threonine
                     protein kinase toxin HipA"
     gene            2147..2248
                     /locus_tag="OO544_004227"
                     /pseudo
     CDS             2147..2248
                     /locus_tag="OO544_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310140.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
BASE COUNT          667 a          504 c          619 g          615 t
ORIGIN      
        1 gagatttggc gcagtggagt agcaacgcgc aggatcgctg gcatcttggc gtgatggcag
       61 gctacgccaa tcagcattag cctgtggggg aatgtcggtg tgcaactagg tgataaaggc
      121 tatagcgata ctcagggcat gctgggagtg aaatatagct ggtaaaccgt ataagccgca
      181 tgtcgagatg gcatgcggct taatattgcc aacttcaaaa ggcttttaaa gcctcccttt
      241 tatccgcgat cgcggatatc gcagcgttta tcccgtacag cggataagat gtgtttccag
      301 attgacttta tcctcactaa aggataaaac tgataatatc cccttaagcg gataaatttg
      361 ctgtggacgt atgacatgat gagctttcag aagatctata gcccaacgca attggcgaat
      421 gcaatgaaac tggttcgcca gcaaaatggc tggacgcaga gcgagctggc gaaaaaaatt
      481 ggcattaagc aggcgacgat ttccaatttc gaaaacaacc ctgacaatac cacgctgacg
      541 acctttttta agattttaca gtcgcttgaa ctctcaatga cgctatgcga cgcgaaaaat
      601 gcctcgccag aagcaacaga acagcaagat ctggagtggt aatgcctaaa cttgtcactt
      661 ggatgaacaa ccagcgggta ggcgagttaa cgaagttagc caacggcgcg cacaccttta
      721 agtatgcacc ggagtggtta gccagccgtt atgccagacc gttgtcactt tcgctgccat
      781 tgcagagggg gaatatcacc tctgatgccg tatttaactt cttcgataac ctgttacccg
      841 atagcccgat tgtacgtgac cggatcgtta aacgttatca tgccaaatcc agacaaccgt
      901 ttgatttgtt gtcagaaata gggcgagaca gcgttggtgc cgtgacgtta atacccgaag
      961 acgaaaccgt aacgcgtccg ataatggcat gggaaaagct tactgaagcc agacttgaag
     1021 aagtattaac ggcttataaa gcagatatcc cgctaggcat gattagagaa gaaaatgact
     1081 ttcgcatctc ggttgctggc gcacaggaga agacagcact gctcagaata ggcaatgact
     1141 ggtgcattcc gaaaggaata acgccgacga cgcacatcat taaattaccg attggcgaaa
     1201 tcaggcagtc caatgcgacg ctcgatctca gccaaagcgt tgataatgag tattactgtc
     1261 tgctgctggc gaaagaactt gggttgaatg ttccggacgc agaaatcatt aaagcgggaa
     1321 gggtgcgcgc gttagcggtc gaacgttttg acaggcgttg gaatactgag cgaacggttt
     1381 tacttcgctt gccacaggag gatatgtgtc agacattcgg tttaccttca tcggtgaaat
     1441 atgaatcaga tggaggccca ggcatcgcgc ggatcatggc gtttttgatg gggtccagcg
     1501 aggcgctgaa agatcgctat gattttatga agtttcaagt gttccagtgg ttgattggcg
     1561 caacggatgg ccatgcaaaa aacttctccg tatttattca ggctggcggc agttatcgac
     1621 tcacgccatt ttacgacatc atttcagcat ttccggtcct tggcggtacg ggaataacat
     1681 cagcgatctc aaactggcaa tggggcttaa cgcatccaaa ggcaaaaaaa cggcaatcga
     1741 taaaatttat ccgcgacatt ttttggcgac agcaaaggtg ctgagattcc cggaagtgca
     1801 gatgcatgaa atcctaagtg actttgccag aatgattccg gcagcactgg ataacgtgaa
     1861 gacttcatta ccgacagatt ttccggagaa cgtggtgacg gcagttgaaa ccaatgtgtt
     1921 gaggttgcat ggacggttaa gccgagaata cggtagtaag tgagatatgg ggtttggtcg
     1981 ttgttaatga gcaatcatga ccggccaacg actggattct cccttggttt atgtttgtgc
     2041 gcataatgta tattatgtta aatcatgtct gagtgatgcg aagtgctttt ctggtagtcg
     2101 ttattcgttc agattaggtg tccttgtgat ttaaagatgt tatcgtggca cgaatatact
     2161 gccattccag gctcgttata ttgctctcgg gcagtccgta gctggtactg caaacgctga
     2221 tgcgaccttc aaagttcaat atctataatt caatcttaag gtggctatac gtcatctgaa
     2281 attgaaacag ggtgtgtgtg gcatggctac gcacacccga gcaattcacc aatgtgaatc
     2341 tcattattaa tgaggagtgg tttgtatttg gagcgctatg aaaaatcaca taatatctaa
     2401 actat
//
LOCUS       DAJTZL010000324         2319 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.327, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000324 DAJTZL010000000
VERSION     DAJTZL010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2319
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.327"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            20..238
                     /locus_tag="OO544_004228"
     CDS             20..238
                     /locus_tag="OO544_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HCR6769663.1"
                     /translation="MVTLFRKKYPRKSRTTEFLFLILFIVLMTPISPLIFVWIIGKII
                     EPVIELYNDVVWASFNTLHNKINPYKES"
     gene            240..605
                     /locus_tag="OO544_004229"
     CDS             240..605
                     /locus_tag="OO544_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR6769664.1"
                     /translation="MALTKKQRAELRMKFGGRCAYCGCELGEKWHADHVKPVIRFDGN
                     MLHQERDDISNMVPACHPCNLHKHCSSLEDYRRIISDGRREFLASGKGKALVRMGLVE
                     MKSDPVVFWFEKYQEGATA"
     gene            602..1267
                     /locus_tag="OO544_004230"
     CDS             602..1267
                     /locus_tag="OO544_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6769665.1"
                     /translation="MTTITKERLQWLANISGRDDIDDIDGGEIRELALIALASLEAEP
                     VAECIVEDGGMCIDGFGEYVGHSLPDGMHELYAAPRMRQPDGWKAVAVAWKVTFTQVD
                     QESNTFTAIYFDKAEVECWVRLHKACDFRADITPLYAAPAVPVAVNDDMAYAFHHALS
                     DSSLGSDEIEEIKTGLRAAFANVTIQPEPIVPDEIGPNDSNTFDYVDGWNACRAAMLK
                     GDK"
     gene            1267..1632
                     /locus_tag="OO544_004231"
     CDS             1267..1632
                     /locus_tag="OO544_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HCR6769666.1"
                     /translation="MINRTKLEHILEYARQQKCIGQLCKIPPGDMVEIVEMAMRKAGN
                     SPVAPAGWISCSDAVPAEYCDVILLDDLGNVFPGSWDKVFCPTRGGNKMAFVDKDGVE
                     VESSTHWMPLPEPPQEVNQ"
     gene            2087..2242
                     /locus_tag="OO544_004232"
     CDS             2087..2242
                     /locus_tag="OO544_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HCR6769667.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRIGQTEVAVF
                     VDYESRE"
BASE COUNT          627 a          494 c          624 g          574 t
ORIGIN      
        1 cccgaaaggg cggtaatgaa tggttacatt atttagaaaa aaatatccgc gaaagagtag
       61 aacaacagaa ttcctgtttc tcattctgtt tatcgtgttg atgacaccga tatccccgct
      121 aatttttgtc tggataatcg gaaaaataat tgagccagtt attgaattgt ataacgacgt
      181 ggtatgggcg tcattcaaca cactgcacaa taaaattaat ccgtataagg aaagctgata
      241 tggcactgac gaaaaaacaa cgtgcagaac tgcgcatgaa gttcggtggt cgctgcgctt
      301 attgcggctg cgaacttggc gaaaagtggc atgcagacca tgtaaaaccg gtcattcgtt
      361 ttgatggaaa tatgcttcac caggaacgtg acgatatatc caacatggta ccagcatgcc
      421 acccatgcaa tctgcacaag cattgcagta gcctggaaga ttatcgacga attatcagtg
      481 atggtcgtcg tgaattcctt gcgtccggga aaggcaaagc gctggttcgt atgggattgg
      541 ttgaaatgaa atctgacccg gttgtgttct ggtttgaaaa atatcaagaa ggggctacgg
      601 catgaccact attaccaaag agcgactgca atggctggct aacatttctg gccgcgatga
      661 tattgacgat atagacggcg gtgaaattcg tgaactggcg cttatcgctc tggcatcact
      721 ggaagcagag cctgtagcgg aatgcattgt tgaagatggg ggcatgtgta ttgacgggtt
      781 cggtgagtat gtgggtcact cgctgcctga tggaatgcat gagctttatg ctgccccgcg
      841 aatgcgacaa cctgatggat ggaaagccgt agctgtagcg tggaaggtga cgtttactca
      901 agttgaccag gaatctaata cgttcactgc tatatatttt gacaaagcgg aagttgagtg
      961 ttgggtacga ctgcataaag catgtgattt tcgggcagat ataacaccgc tttacgcagc
     1021 gccggcagtg ccggttgcag taaacgacga catggcttac gcattccatc acgcactgtc
     1081 agattcatcg cttggctctg atgaaatcga agaaattaaa accggtttgc gtgctgcctt
     1141 tgccaacgtc actatccaac cagagccgat agtgccggat gaaatcgggc caaacgatag
     1201 taatacgttt gattatgttg atggctggaa cgcctgccgc gctgccatgc ttaagggaga
     1261 taaataatga ttaatcgaac caaactggag cacattctcg aatatgccag gcagcagaaa
     1321 tgtattgggc aactttgtaa aattccacca ggagatatgg ttgaaatcgt ggaaatggcc
     1381 atgcgtaagg ctggcaactc tccggtagct ccggctggct ggataagctg tagtgatgca
     1441 gttcctgctg aatattgcga tgtgattctt ctcgatgatc tcgggaatgt attccccggt
     1501 tcctgggata aggttttttg ccccactcgt ggcgggaata agatggcttt tgtggacaaa
     1561 gacggcgttg aagtagagag ctcaactcac tggatgccgc taccggaacc accgcaggag
     1621 gtgaatcaat gacctggcct gaggcattca ccacggtagg aattgcgatg gcggtggcgc
     1681 tggtggtgta ttcgatttgc cgttggggtt aacaaacaaa aaccccggat tgatggtccg
     1741 gggtttttga aggaaacaaa acagaaacag caattgccgt tacctgttgt taccatggca
     1801 agtaaacgta tctcaggcga gcgcattgcg ccgttctgac gcagataaac tagcctggat
     1861 agatggtgct ggcaataaaa aatagcgttt tcttatcggt gtcggtaaga ttgctgcggg
     1921 tgcttgaggc tgtctgcctc gggtatgtca ctgtaaggca gacagagaaa agccccagtt
     1981 aacattacgc gtcctgcaag acgcttaaca ttaatctgag gcccaatcta tgtctcacaa
     2041 atgtaggtta gcctcttacg tgccgaaagg caaggagaag caggctatga agcagcaaaa
     2101 ggcgatgtta atcgccctga tcgtcatctg tttaaccgtc attgtgacgg cactggtaac
     2161 gaggaaagac ctctgcgagg tacgaatccg aatcggccag acggaggtcg ctgttttcgt
     2221 agactacgaa tctagagagt aagagtgacc aggcgggaga gtaatctccc gccacctttg
     2281 atgtgtcagg catcctcaac gcacccgcac ttaacccgt
//
LOCUS       DAJTZL010000325         2286 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.328, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000325 DAJTZL010000000
VERSION     DAJTZL010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2286
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.328"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..325
                     /gene="casB"
                     /locus_tag="OO544_004233"
     CDS             <1..325
                     /gene="casB"
                     /locus_tag="OO544_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HCR6769668.1"
                     /translation="VSANVKAIDERQPFAAQLAAVMSKGRFTRLSAVKTPDELLRQLR
                     RAVRLLNGSVNLDSLADGIFRWCQESDDLLNHHRRQQRPTEFIRIRWALEYYQAGDAD
                     NEQNQ"
     gene            337..1392
                     /gene="cas7e"
                     /locus_tag="OO544_004234"
     CDS             337..1392
                     /gene="cas7e"
                     /locus_tag="OO544_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HCR6769669.1"
                     /translation="MTTFIQLHVLTAYPAANLNRDDTGAPKTVVLGGATRLRVSSQSL
                     KRAWRTSALFEQALAGHIGIRSGRIAREAATILIEKGIEDKKAIEWAAKIADYLGKAK
                     NDKKPKDPLTNAETEQLVHISPAEFDAVKALAHQLAEEKRAPKEEDLALLRKDRIAVD
                     IAMFGRMLANKPEFNVEAACQVAHAFGVSETIVEDDFFTAVDDLRQASEDAGAGHLGE
                     TGFGSALFYTYICIDKDLLVENLGGDEALANQTIRAFTEAALKVSPTGKQNSFASRAY
                     ASWALAEKGTDQPRSLAAAFYEPINGTRQLDVAVQRITTLRENMNTVYEQKTECASFD
                     VMNKQGSMKDVLDFICA"
     gene            1403..2149
                     /gene="cas5e"
                     /locus_tag="OO544_004235"
     CDS             1403..2149
                     /gene="cas5e"
                     /locus_tag="OO544_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HCR6769670.1"
                     /translation="MSQYLIFQLHGPMASWGVDAPGEVRHTHELPSRSALLGLLAAGV
                     GIRRDDTERLNAFNRHYSLVVCASRNPRWARDYHTVQMPKEVRKARYFSRREELSDPE
                     LLSAIISRRDYYTDAWWMVAVATTADAPYSLEQLQDGLRHPVFPLYLGRKSHPLALPL
                     APLLLEGNACDALCNAYQQYQDHFHKLKVSLPKLQDECWWEGKHDGLVASKILRRRDV
                     PLNRQQWLFGERTVNQGPWLSKEEPCTSQE"
     gene            2131..>2286
                     /gene="cas6e"
                     /locus_tag="OO544_004236"
     CDS             2131..>2286
                     /gene="cas6e"
                     /locus_tag="OO544_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311637.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HCR6769671.1"
                     /translation="MYLSRITLHTGQLSPAQLLHLVDRGEYVMHQWLWDLFPGGKERQ
                     FLYRREEL"
BASE COUNT          569 a          570 c          613 g          534 t
ORIGIN      
        1 agttagcgcc aatgttaaag ccattgatga acggcagcct tttgccgcgc aactggcggc
       61 ggtaatgtca aaggggcgtt ttactcgcct gtcagcagta aaaacaccgg atgaattact
      121 gcgccagtta cgccgcgccg tcagattatt aaacggttcc gtcaatcttg actccctggc
      181 agatggcatt ttccgctggt gccaggagag cgacgacctg ctcaatcatc accgccgcca
      241 gcagcggcca accgaattta tccgtatccg ctgggcgctg gaatattacc aggctggcga
      301 cgccgataac gaacaaaatc aataaaggac tatctcatga caacatttat tcagcttcat
      361 gtgttaaccg cttaccctgc tgccaacctt aaccgtgatg acaccggtgc gccaaaaact
      421 gtggtgctgg gtggagcaac gcgtctgcgc gtttcctcgc aaagtctgaa acgtgcgtgg
      481 cgcacttctg cactttttga acaggcactg gcgggccata ttggtattcg cagtgggcgt
      541 attgcgcgtg aggcggcaac tatcctgatt gagaaaggga ttgaagataa aaaagccatc
      601 gaatgggcgg caaaaattgc tgattatctt gggaaagcta aaaatgacaa aaaaccaaaa
      661 gatccgctca ctaacgccga aactgaacaa ttagttcata tcagcccggc agaatttgac
      721 gccgtaaaag cgcttgctca tcaactggca gaagaaaagc gcgcgccaaa agaggaagat
      781 ctcgcgttgt tacgtaaaga ccgtatagcg gtagatatcg ctatgtttgg tcgtatgctg
      841 gcgaataaac ctgagtttaa tgttgaagcc gcctgccagg tcgcgcatgc gtttggtgtc
      901 agtgaaacga ttgtcgaaga tgattttttc accgccgttg atgatttgcg ccaggcctct
      961 gaagatgccg gtgccgggca tctcggagaa accggattcg gttcagcact tttctacacc
     1021 tatatctgca ttgataaaga tctgctggta gaaaacctcg gcggtgacga agcgttagct
     1081 aatcagacca tacgcgcttt tacggaagcc gcgcttaaag tttccccaac cggtaaacaa
     1141 aatagctttg ccagccgtgc ctatgcttcc tgggcgctgg cagaaaaagg caccgatcaa
     1201 ccacgctctc tggcggcggc tttctatgaa cccattaatg gcacacgtca gttagatgtg
     1261 gcggtgcagc gtattacaac gcttcgcgaa aacatgaata cggtctacga acagaagacc
     1321 gaatgcgcaa gttttgacgt gatgaacaaa cagggaagca tgaaggacgt gctggacttt
     1381 atctgcgcgt aaggggcggc ttatgagcca atatctgatt tttcagcttc atgggccgat
     1441 ggcgtcctgg ggtgtcgatg ctccaggtga agttcgtcat acccatgaac ttccttcgcg
     1501 ctcagcattg ctggggctac tggctgccgg ggtagggatt cggcgtgatg ataccgaacg
     1561 cttaaacgcg tttaatcgcc actattcact ggtggtttgc gccagccgta acccgcgctg
     1621 ggcacgggat tatcacacgg tccagatgcc aaaagaggtg cgtaaagcac gttatttcag
     1681 ccgtcgtgaa gagttgagcg accctgagct tttgagcgcg attatctccc ggcgcgacta
     1741 ctacaccgat gcctggtgga tggtggcggt ggcaacaaca gccgatgcgc cttacagcct
     1801 cgaacagttg caggacggtt tacgccatcc agtttttccg ctttatctgg ggcgaaaaag
     1861 tcatccgctg gcattacctc ttgcgccgtt actgctcgaa ggcaacgcgt gtgatgcctt
     1921 atgtaatgca taccaacagt atcaggatca tttccataaa ctgaaagtct cactcccgaa
     1981 acttcaggat gaatgctggt gggaagggaa acacgacggc ctggtcgcga gcaaaatatt
     2041 acgccgccgg gatgttcctt taaatcgtca gcagtggctg tttggggaac gcaccgtcaa
     2101 tcaggggccg tggctcagca aggaggaacc atgtacctct caagaataac cttacatacc
     2161 ggccagcttt cgcctgcgca gttgctgcat ctggtggatc gcggtgaata tgtgatgcat
     2221 cagtggctgt gggatctttt ccctggcggc aaagaaaggc aatttcttta tcgtcgggaa
     2281 gaactc
//
LOCUS       DAJTZL010000326         2242 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.329, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000326 DAJTZL010000000
VERSION     DAJTZL010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2242
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.329"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(1118..1453)
                     /locus_tag="OO544_004237"
     CDS             complement(1118..1453)
                     /locus_tag="OO544_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin transporter"
                     /protein_id="HCR6769672.1"
                     /translation="MNRKYYFNNMWWGWVTGGYMLYMSWDYDFKYRLLFWCISLCGMV
                     LYPVAKWYIEDTALKFTRPDFWNSVFFADTPGKMGLLAVYTGTVFILSLPLSMIYILS
                     VIIKRLSVR"
     gene            1582..1929
                     /locus_tag="OO544_004238"
     CDS             1582..1929
                     /locus_tag="OO544_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6769673.1"
                     /translation="MTFEQKKARAIALMDSKKMWRSNYAPPLLRILWRLGIRLPPLPF
                     MPFWQVTVLTGGLWGISWGCAMWFIYWGPSGMVAGEAIIISITGGFLSGLLMASFHWW
                     RRKVNRLPPWDDV"
     gene            complement(1949..>2242)
                     /locus_tag="OO544_004239"
     CDS             complement(1949..>2242)
                     /locus_tag="OO544_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6769674.1"
                     /translation="EQRAAEVVVLPLVPAAGLPAGGTSATDLAVELNGITYQACRGDF
                     VVRLDGSTCLQLWNKEGWVVRREGDPLEVAQWLPACHDAGMEVRVQINESAAP"
BASE COUNT          688 a          490 c          420 g          644 t
ORIGIN      
        1 tcacataaac caccgattga gtgacagtta ctgaaacaac tatttcgaca caaaatctcg
       61 tctattcaac atcatgcaaa tttgtcagtg actgaattta gtgacagaac cctctggaat
      121 tctgccgtgt aaccacttat atgcagacct gccccgttgc tctgggtttt caggctgcga
      181 tgagtgatga gcgatgattt ccatacagat gatatatcga ctggaattga ttgcctgcaa
      241 ttagatttag ttttattgtg tggatcgttt tagttagtgg ggatgaattt ccgataaatt
      301 aaccaatgga aaggagatga tatgaataaa aagataactg gattattaat aattagtctg
      361 gcgtcaattt tcttaattga ttgcacccag tgccattaac atctatctac acaaaaatag
      421 aggaaaacaa ttttgatata tcaaataatt acaagcagct ccattctttt tattgtttgg
      481 gggattacat ctataataat tagaacaaag aattattttt ctaagaagaa aacagacctt
      541 aatataagct tttttatagc cttccctatt tctataacaa tccttttatt ttgccgaggt
      601 atattataaa tataacataa tacatctata tggggtgtac atatccccac ataaaaacac
      661 atactctcat taacacggga agaaactatg ttttccaatt ctcactacat gtacataatc
      721 gttcttgtac tatggtttag tattctcaag tacttacaga gaaagaatag catattacat
      781 cctttatttt cactaatcct gacaccacta accattttaa tcattagact tatgatagat
      841 ttaacataca accaataaat tataaacaaa ataaaaatag ggaacattat atgctttata
      901 aaattttcac attaaacaca aagataaaaa caactatcat tacaatatgt gcattatcat
      961 ataatcttac agcgcaagct tccccgtcat acagacctcg tgatcgagtt aataagacta
     1021 tgatagaact acaaactaac ggagtagaac ggcaactttt agataattct tgtggcataa
     1081 gttctggggt atttaaataa aatataagac aggctgtcta tcttacagac agccttttta
     1141 taataacaga aagaatatat atcatactta acggaagaga taatatgaaa acagtacccg
     1201 tataaacagc aagcaatccc atttttccag gtgtatcagc aaaaaaaacg ctgttccaga
     1261 aatcaggccg ggtaaatttc agagctgtat cttcaatata ccattttgca accggataca
     1321 aaaccattcc gcagagagaa atacaccaga acagtaatct gtatttaaaa tcataatccc
     1381 atgacatata cagcatatat cctcccgtca cccatcccca ccacatatta ttaaaataat
     1441 attttctgtt catctgccag ttttcctttc ttttgcactt ttgtcagtgt actgatgcat
     1501 gacaacataa ccacagcatg ataataataa tcaataacaa taagctgtgt cacgtttaca
     1561 tcatggaacg gaggtgctcc tgtgactttt gagcagaaaa aagcccgggc cattgccctg
     1621 atggacagca aaaagatgtg gcggagcaat tatgcccctc ctcttctgcg tattctctgg
     1681 cggctgggta tccggcttcc gccattaccc tttatgccgt tctggcaggt cacggtgctg
     1741 acggggggct tatggggcat ttcctgggga tgtgcaatgt ggtttatcta ctgggggccg
     1801 tcaggaatgg tggcaggtga ggccattatt ataagtatca cggggggttt tttgtccgga
     1861 ctgctcatgg cctctttcca ctggtggcgc aggaaagtta accgactgcc accatgggat
     1921 gatgtgtaaa ccgtgcacag gcgtgttctt acggagcggc actttcattt atctgtacgc
     1981 gaacctccat tcctgcatcg tgacaggccg gcagccactg cgccacttcc agcggatcgc
     2041 cctcccggcg taccacccag ccttctttat tccataactg cagacaggtg ctgccgtcga
     2101 gacgcaccac aaaatcccca cggcaggcct gatacgttat gccgttcagc tccaccgcca
     2161 gatcagtggc agaagtgccg cctgcgggca gacctgctgc cgggaccagc ggcagtacga
     2221 cgacttctgc cgcacgctgt tc
//
LOCUS       DAJTZL010000327         2241 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.330, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000327 DAJTZL010000000
VERSION     DAJTZL010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2241
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.330"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..312)
                     /locus_tag="OO544_004240"
     CDS             complement(<1..312)
                     /locus_tag="OO544_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCR6769675.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWLEDGKGSTTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYHRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEAG
                     LA"
     gene            complement(331..2208)
                     /gene="bglF"
                     /locus_tag="OO544_004241"
     CDS             complement(331..2208)
                     /gene="bglF"
                     /locus_tag="OO544_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HCR6769676.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     NTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFWFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADALGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGPLSTWISELIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHWGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTVMLH
                     FITAKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDVTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
BASE COUNT          592 a          625 c          525 g          499 t
ORIGIN      
        1 cgctaacccc gcttcattcg gttcggcttc gtcgccctga gggaaaattc gcgcccaggc
       61 aatggaaata cgcagacagg tgaagcccat ctcggcaaat aacgcgatat cttccgggta
      121 acggtgataa aaatcgatgg cgacatcttt gatattctct ttccccagga tgcgcggttc
      181 catttttccc ataacgccat gaggctgtaa atctgaggtc gtgctccctt tgccatcttc
      241 cagccaggca ccttccacct gattggcagc tgttgcgcca ccccaaagaa atgtttctgg
      301 aaatgctttc ataattaact ccttttatcg ttagcgaatg atggataaca gcggttcacc
      361 tgcgcttatc tgcgccgtgc cgtggggtaa tacgtctgta aaatcatcgc tattactgat
      421 taataccggc gtcgtcacat caaatccggc ctcgcgaata gcagggatat caaaagaaat
      481 cagccgatcg cctgtattga ccttgtcacc cacgttgacg tgagcggaaa agaatttgcc
      541 gtccagtttt acggtgtcga taccgacatg aatcaggatc tccacaccat catctgactc
      601 aatgccaatg gcgtgtaatg tggcgaacaa cgaagcaatt cgacccgcaa ccggagaacg
      661 cacttcacca accgagggca gaatggcgat acctttaccc aacaggccac tggcaaacgt
      721 ggtatcagcg acgtgaatga gcggcacaat ctctcccgtc atcggtgaac agataccgcc
      781 ctgctcaggt ggtgtaataa cctctggtgt tttctcttgc ggggcaccct gcgctggctg
      841 acgtttagcg gtgatgaaat gaagcatcac cgtaccgaca aatgcgcaac cgatggcaat
      901 gacaccgcca ataacgctgg cccagacggt gaaatcaatt cccgttgacg ggatggtttg
      961 catgaaggtg aaaatacttg gcaaaccaaa ggagtacact ttcgtttgcg cgtagccaat
     1021 gatggtggcc cccaaagccc cactgataca ggcgataaca aaggggtact tacgcggcag
     1081 attaacgcca tataccgctg gttcggtgat accaaacagg cccgtcaacg ccgctgatcc
     1141 cgccaccact tttttctgcg catcgcgttc gcagaggaag acgccgagcg ccgccccgac
     1201 ctgcgccata atggcgggca ttaacagcgg gatcatggtg tcgtagccca gcacggtgaa
     1261 gttattgata cacagcggca ccaggcccca gtgcagtccg aacatgacga agatttgcca
     1321 gaagccgccc attaccgcgc ccgcaaatgc aggaaccgcc tgataaagcc agagataacc
     1381 ggcggcaatc agttcgctta tccaggttga tagcggcccc accagcagaa aggtgacggg
     1441 tgtgataacc atcagacata gcaatggtgt gaagaaattt ttgattgccg acggtaacca
     1501 cgcattaagt cggcgttcca gaatgctgca caaccaggca gaaaaaataa tgggaataac
     1561 cgatgacgag taattcaaca atgtgaccgg aatacccagg aaatccagcc ccagcgcatc
     1621 cgctttttgc ccgttctcga aagcagtcag aattaatgga tgcactaacg ctccaccaat
     1681 caccatggca gtaaatggat taccgccaaa gcgtttcccc gcggtgtatc ccaggattat
     1741 cgggaagaac caaaacaagg catcactggc gctgaataaa attaaataag taccactttg
     1801 ttcggtcgtc cactgaaaag tgagcgccag agccagcata cctttcagga tcccggttgc
     1861 cgccatcaaa ccgatcagag gcgtaaaaat acctgaaata acataaacaa agcggtttag
     1921 cagattacct ttctcatcat tttccggtgc ctgttgcgct ttttcgccaa ggcctgccac
     1981 gctgttgacc gccaggaaga catcggccac atggttacct atgaccacct gaaactggcc
     2041 accgctttcc accaccataa taataccggg ggtgtttttc agtacctcag cttgcgcttt
     2101 gctttcatcc tttaacttaa aacgtaatcg cgttgcgcaa tgcatcagac taacaatgtt
     2161 atctgcgccc ccgactcctg cgactatttt tctggctaac tccgtcataa cttgccctct
     2221 accgctttgc ggcaaaactc c
//
LOCUS       DAJTZL010000328         2170 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.331, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000328 DAJTZL010000000
VERSION     DAJTZL010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2170
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.331"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>2170
                     /locus_tag="OO544_004242"
     CDS             <1..>2170
                     /locus_tag="OO544_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="short-chain fatty acid transporter"
                     /protein_id="HCR6769677.1"
                     /translation="KSAAAAESSKSAAATSAAAAKTSETNAAASQQSAATSASAATTK
                     ASEAATSARDAAASKEAAKSSETNASSSASSAASSATAAGNSAKAAKTSETNARSSET
                     AAGQSASAAAGSKTAAASSASAASTSAGQASASATAAGKSAESAASSASTATTKAGEA
                     TEQASAAARSASAAKTSETNAKASETSAESSKTAAASSASSAASSASSASASKDEATR
                     QASAAKGSATTASTKATEAAGNATAAAQSKSTAESAATRAETAAKRAEDIASAVALED
                     ASTTKKGIVQLSSATNSTSETLAATPKAVKSAYDNAEKRLQKDQNGADIPDKGRFLNN
                     INAVSKTDFADKRGMRYVRVNAPAGATSGKYYPVVVMRSAGSVSELASRVIITTATRT
                     AGDPMNNCEFNGFVMPGGWTDRGRYAYGMFWQYQNNERAIHSIMMSNKGDDLRSVFYV
                     DGAAFPVFAFIEDGLSISAPGADLVVNDTTYKFGATNPATECIAADVILDFKSGRGFY
                     ESHSLIVNDNLSCKKLFATDEIVARGGNQIRMIGGEYGALWRNDGAKTYLLLTNQGDV
                     YGGWNTLRPFAIDNATGELVIGTKLSASLNGNALTATKLQTPRRVSGVEFDGSKDITL
                     TAAHVAAFARRATDTYADADGGVPWNAESGAYNVTRSGDSYILVNFYTGVGSCRTLQM
                     KAHYRNGGLFYRSSRDGYGFEEDWAEVYTSKNLPPESYPVG"
BASE COUNT          576 a          540 c          612 g          442 t
ORIGIN      
        1 caaaaagtgc tgccgctgca gagtcctcaa aaagcgcggc ggctaccagt gccgctgcgg
       61 cgaaaacgtc agaaacgaat gcggcagcgt cacaacaatc agcagccact tctgcatccg
      121 ccgcgaccac gaaggcgtca gaagcagcca cctcagcccg ggatgcggcg gcctcaaaag
      181 aggcagcgaa atcatcagaa acgaacgcat catcaagcgc cagtagtgcc gcttcctcgg
      241 caacggcggc aggcaattcc gcgaaggcgg caaagacgtc tgagacaaac gccaggtctt
      301 ctgaaacggc agcgggacag agcgcctcgg ctgcggcagg ctcaaaaaca gcggctgcgt
      361 cgtctgccag tgccgcgtca acaagtgccg ggcaggcctc agccagtgcc accgccgccg
      421 gaaaatcggc agaaagtgcc gcatcgtctg cttcaacagc cacaacgaag gctggcgaag
      481 ccactgaaca ggccagcgca gcagcgaggt ctgcttccgc agcgaagaca tccgagacga
      541 acgcgaaagc gtcggaaacc agcgcagaat cctcaaaaac ggctgccgca tcgtccgcca
      601 gttcggcggc gtcatcggca tcatcggcgt ctgcttcaaa agatgaggcg accagacaag
      661 cgtccgcagc gaagggcagc gccacgacag catccacgaa ggcgacagag gcagctggca
      721 atgcgacggc ggcagcacag agcaaaagta cggcggaatc cgcggcaacg cgcgccgaga
      781 cagcggcaaa acgggcagag gatattgcat ccgccgtggc gcttgaggat gcaagtacga
      841 cgaaaaaggg gatagtacag ctcagcagtg cgaccaacag tacgtctgaa acgctggcgg
      901 caacgccaaa ggcagtaaaa tcagcctatg acaatgcaga gaaacgtctg cagaaagacc
      961 agaacggcgc tgatataccc gataagggac gcttcctgaa caacattaac gcggtcagta
     1021 aaacagactt tgctgataag cgtggtatgc gttatgtgcg ggttaacgct cctgcaggtg
     1081 caacatctgg aaaatattac cctgttgttg ttatgcgttc tgctggctca gtaagcgaac
     1141 tggcatcaag ggtcattatc accacggcaa cgcgaaccgc aggcgatccg atgaataact
     1201 gcgagtttaa cggatttgtt atgcctggtg gctggactga cagggggcgt tatgcttatg
     1261 gaatgttctg gcaatatcaa aacaatgaac gagccatcca ctcaataatg atgagtaata
     1321 agggcgatga tttgcgctct gtgttctatg ttgatggcgc tgctttccct gtttttgcgt
     1381 ttatcgaaga tggcctgtca atatccgcac ctggtgctga tctcgttgtt aatgatacga
     1441 cctataagtt tggggcaaca aatccggcga ctgaatgtat cgcggcggac gttatccttg
     1501 attttaagag tgggcgtggt ttttatgagt ctcattcgtt aatcgttaac gataacttgt
     1561 cgtgcaaaaa actttttgcc acagacgaaa ttgtagcgcg tggtggtaat cagattcgaa
     1621 tgataggtgg ggagtatggg gcattatggc gtaatgatgg cgctaaaact tacctgctgc
     1681 ttaccaatca aggtgatgtt tatggtggct ggaatacatt aagaccgttt gctattgata
     1741 acgcaaccgg cgaactggtt attggaacca aactgtccgc aagtctgaac ggtaatgcat
     1801 taacagcaac aaagctgcaa acgccaagac gggtttctgg tgttgagttt gatggttcca
     1861 aagatattac tttaaccgcc gcgcatgtgg ctgcttttgc cagaagggca acggatacat
     1921 atgccgatgc ggatggtggc gttccctgga atgccgaatc aggcgcttat aatgtcaccc
     1981 gctctggcga cagctatatt ctggttaact tctataccgg agtcggaagt tgccggacct
     2041 tgcagatgaa ggcgcattac agaaatggtg gtctgttcta ccgttcttca agagacggtt
     2101 atggttttga ggaagactgg gcagaagttt atacctcgaa aaatcttcca ccagaaagct
     2161 acccagtcgg
//
LOCUS       DAJTZL010000329         2150 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.332, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000329 DAJTZL010000000
VERSION     DAJTZL010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2150
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.332"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(89..652)
                     /locus_tag="OO544_004243"
     CDS             complement(89..652)
                     /locus_tag="OO544_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HCR6769678.1"
                     /translation="MNRTSIEKLPSLTHNHLPVMTTELLADLYGTERQRLTNNFNRNK
                     ERFIEGKHFFLIEGDALRELKNENSLRVSVKIARNVRSLILWTERGAARHAKMLETDQ
                     AWEVFEKLEDCYFNQKQPPAAQNTSIENDGCALLSHFDKHGQVEFTEKVPADAMVCTL
                     ERFKFYLEQHGWIVARKEQLVERLMRF"
     gene            complement(756..974)
                     /locus_tag="OO544_004244"
     CDS             complement(756..974)
                     /locus_tag="OO544_004244"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6769679.1"
                     /translation="MGELKSSPVGFPVVPTLCVSPPDDWNLTVLTVSGLQFTFPRGAQ
                     NTSKAVSTPDSHVVFLCPEFLVYDRVCG"
     gene            complement(1133..1519)
                     /locus_tag="OO544_004245"
     CDS             complement(1133..1519)
                     /locus_tag="OO544_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCR6769680.1"
                     /translation="MARDIQQVMERWGAWAANTHEDVSWASIAAGFKGLIPSKVKSRP
                     QCSDDDAMIICGCMARLNKKNQDLHDLLVDYYVGGMTFMSLARKHGCSDTCIGKRLQK
                     AEGVIDGMLMMLDIRLEMDRYVERIM"
     gene            complement(1509..1880)
                     /locus_tag="OO544_004246"
     CDS             complement(1509..1880)
                     /locus_tag="OO544_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCR6769681.1"
                     /translation="MRHEFILPYPPTVNTYWRRRGSTYFVSKVGERYRRDVTLIVRQQ
                     RLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVRGQ
                     LVPGGRLGVKIYEITGDNDGA"
     gene            complement(1880..2137)
                     /locus_tag="OO544_004247"
     CDS             complement(1880..2137)
                     /locus_tag="OO544_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6769682.1"
                     /translation="MNNQTMTFTSEQLRKHAQEMLRQAEQLEKTGVTKDAIRRDMVPA
                     LRELMQAKHRAQKAVDELVDCVAELETKVGKFEKMVQEVLR"
BASE COUNT          527 a          579 c          463 g          581 t
ORIGIN      
        1 catcggcggg tttaagctgt gtggcgtagt aaccactctt aacaggatat tcaacttttt
       61 acgatcgtaa agttttcgga aaaaattttt aaaaccgcat caaccgctcc accagttgct
      121 ctttacgggc aacgatccac ccgtgttgct ccagataaaa tttaaaccgt tccagagtac
      181 ataccatcgc atcggcgggt accttttccg tgaactcaac ctgaccgtgt ttatcgaagt
      241 ggctcagtaa tgcgcatcca tcattttcga tagatgtgtt ttgtgccgct ggtggctgtt
      301 tctggttgaa ataacagtct tccagttttt cgaacacttc ccacgcctga tcggtttcga
      361 gcattttggc gtgacgggct gcgccacgtt ctgtccagag gatgagggag cgaacgttac
      421 gggcaatttt cacagaaact cttaaagagt tttcgttctt caactctcgt aacgcatcac
      481 cttctatcag gaaaaaatgt ttaccttcta taaaccgttc tttgtttcga ttgaaattgt
      541 ttgtcaaacg ctggcgctct gttccgtaca aatcagccag cagctctgtt gtcatgacag
      601 gaagatggtt atgcgtaagt gacgggagtt tttcaataga cgtgcgattc atagacatgt
      661 cctttcgaat attttgattt accccttttg agagggtgac cgggcgctca aaaccgtcga
      721 aagtcggcgg gcgtatttcc attgctggta ttgtattagc cgcacacccg gtcataaacc
      781 aagaattctg gacacaaaaa aaccacatgg ctatcgggtg tggataccgc tttcgaggtg
      841 ttttgagcac cgcggggaaa tgtaaactgc aaacctgaaa ctgtcaacac cgtcaggttc
      901 caatcatcgg gtggtgagac gcacagggtt ggaactaccg ggaaaccgac cggcgagctt
      961 ttcagctccc ccatgcgccc caccataatt cagatgtgcg cgtgcatacg acaataaaaa
     1021 acacgctcgc ggcgtgtgtc tgtcgcggtc tctatccggg gttccaatcc cgacgccaga
     1081 ttttgctggc gcgtgaggaa tatagccccg gacaatgtgt ctggtcaagc acctacataa
     1141 ttcgttctac gtatctgtcc atctccagcc ggatatcaag catcatcaac atgccatcaa
     1201 taaccccttc cgctttctgc aggcgcttgc caatacaggt atccgaacac ccatgtttcc
     1261 gtgccagact cataaaagtc attccaccta cgtaataatc caccagcaaa tcgtgcaaat
     1321 cctgattttt cttgttcaac cgggccatac agccacaaat tatcattgca tcatcatcag
     1381 aacactgagg gcgtgatttc actttcgacg gaattaaccc tttaaaacca gcagcgatcg
     1441 acgcccagga tacatcttca tgagtgtttg cagcccaagc cccccaccgt tccataacct
     1501 gctgaatatc acgcgccatc gttatcacct gtgatttcat aaatcttcac gcccagtcgc
     1561 ccaccaggaa cgagctgacc gcgcacaata ttgatttcat caaactgctc gtcgtctatg
     1621 agtagtccgg catgcgtcag cgcatccagt ggtgctttca ggatattgtc caggtcacga
     1681 cggcgcttat ccggtggctc tgcaataatc tttatcgcca gccttccgga caggtttaat
     1741 ttcagccgct gctggcgaac aattagcgtc acatcacggc gataacgctc accaactttt
     1801 gatacaaaat atgtgctgcc acgacgtcgc cagtaagtat tcaccgtcgg cggataaggt
     1861 aaaataaact catggcgcat cagcgcagca cctcctgcac cattttttca aactttccaa
     1921 ctttggtttc cagctctgcc acacaatcaa cgagctcatc caccgctttt tgtgcgcgat
     1981 gtttcgcctg catcagttcc ctgagcgctg gcaccatatc ccgacgaatg gcatcttttg
     2041 ttacacctgt tttttccagt tgttccgcct gtcgcaacat ttcctgcgcg tgtttacgca
     2101 attgttcaga ggtaaaagtc attgtctggt tgttcaaaag aaacgctcca
//
LOCUS       DAJTZL010000330         2109 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.333, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000330 DAJTZL010000000
VERSION     DAJTZL010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2109
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.333"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..405)
                     /gene="ratA"
                     /locus_tag="OO544_004248"
     CDS             complement(<1..405)
                     /gene="ratA"
                     /locus_tag="OO544_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HCR6769683.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELASNMVQAFT"
     gene            576..1058
                     /gene="smpB"
                     /locus_tag="OO544_004249"
     CDS             576..1058
                     /gene="smpB"
                     /locus_tag="OO544_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HCR6769684.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            1273..1635
                     /gene="ssrA"
                     /locus_tag="OO544_004250"
     tmRNA           1273..1635
                     /gene="ssrA"
                     /locus_tag="OO544_004250"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1834..>2109)
                     /locus_tag="OO544_004251"
     CDS             complement(1834..>2109)
                     /locus_tag="OO544_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014334053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HCR6769685.1"
                     /translation="GRIWNAGPDSLGRLSPVVMLAKSVTAQTHMAWGDADNQQVKLSM
                     PELEELAAAMVQAQVDRNDEIYRRQREMKEELSGLEDLGTIRAFDVT"
BASE COUNT          595 a          558 c          478 g          478 t
ORIGIN      
        1 cgtaaaagcc tggaccatat tagacgccag ctctttaaac acgcgaccaa aggcgagttc
       61 aatcaactta ttggtaaact caaagtcgag atgaaactcg atacgacacg cctcctggct
      121 cagcggcgta aacttccatc caccaatcaa tttcttgaac ggcccatcca ccagattcat
      181 aagaatactt tggttactgg tcaactggtt gcgggtagta aacgttttgc tgatcccagc
      241 cttagagaca tctaccgccg cagtcatctg cccaggagtg gactccagaa tccgacttcc
      301 ggtacaaccc ggcaaaaact gaggataaga ctgaacgtca ttcactaact gatacatttg
      361 ctccgcgctg tagggtacca gtgcggtccg gctaatctga ggcataacta tttccatcaa
      421 caaaaatcca acaaataata tcatttatcc tgctgtaaaa aaaacgctat cccggcgctg
      481 ggtaacatcg ggttcatgct aagatagagc cttgtccccc gcaggattga tatggggtgt
      541 tttcgatttc agattaccga tgattcacga cgcttatgac gaagaaaaaa gcacataaac
      601 ctggttcagc gaccatcgcg cttaacaagc gcgcccgtca cgaatacttt atcgaagaag
      661 agttcgaagc gggacttgcc ctgcaaggct gggaagttaa atccctgcgc gcaggaaaag
      721 ccaatatcag cgacagctac gtccttctgc gtgacggaga ggcatttctg tttggcgcta
      781 acatcacgcc aatggccgtg gcctccacgc atgtggtgtg cgatcctacc cgtacccgca
      841 agttacttct caaccagcgc gaactggact cattgtacgg tcgcgtcaat cgagaaggct
      901 ataccgtagt ggcgctctcc ctgtactgga aaaatgcctg gtgcaaagtg aaaatcggcg
      961 tcgccaaagg taaaaaacag cacgataaac gttcagatat caaagaacgc gaatggcagg
     1021 tggataaagc acgtatcatg aaaaacgccc accgttaaac ctgcactcca attattgacc
     1081 agttcctcac cgcgcctccc tctccggcgg cgcgaatgaa catcttattg gctatcacat
     1141 ccgacacaaa tgttgccatc ccattgctta atcgaataaa aatcaggcta catgggtgct
     1201 aaatctttaa cgataacgcc attgaggctg gtcatggcgc tcataaatct ggtatactta
     1261 cctttacaca ttggggctga ttctggattc gacgggattt gcgaaaccca aggtgcatgc
     1321 cgaggggcgg ttggcctcgt aaaaagccgc aaaaaatagt cgcaaacgac gaaaactacg
     1381 ctttagcagc ttaataacct gcttagagcc ctctctccct agcctccgct cttaggacgg
     1441 ggatcaagag aggtcaaacc caaaagagat cgcgtggaag ccctgcctgg ggttgaagcg
     1501 ttaaaactta atcaggctag tttgttagtg gcgtgtccgt ccgcagctgg caagcgaatg
     1561 taaagactga ctaagcatgt agtaccgagg atgtaggaat ttcggacgcg ggttcaactc
     1621 ccgccagctc caccactttt gacaggactg caaccggaca gagactataa aaacagtcac
     1681 ttacggacac tgaccagaca gtaagcggac cagaaaaaga caaaaatata cacgcaaaat
     1741 gcacgtgcat ttcaaaaaag cctctgatta tgaagtcaga ggcttttcta gttgtaaata
     1801 agggtaacaa tttaacacga tgcagcttac caattacgta acatcaaacg ctctaattgt
     1861 tcccaaatcc tccagaccac tcagctcctc tttcatttca cgctgacggc gataaatctc
     1921 atcgttgcga tcgacctgcg cctgcaccat tgctgccgcc agttcttcca gttccggcat
     1981 cgacagtttc acctgctgat tatcggcatc accccacgcc atatgcgttt gtgctgtgac
     2041 agattttgcc agcatgacta ccggggacag gcggcccagt gagtcggggc cagcattcca
     2101 gatacgacc
//
LOCUS       DAJTZL010000331         2109 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.334, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000331 DAJTZL010000000
VERSION     DAJTZL010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2109
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.334"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..512)
                     /locus_tag="OO544_004252"
     CDS             complement(<1..512)
                     /locus_tag="OO544_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HCR6769686.1"
                     /translation="MGKYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSVGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSA
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIIDAELFALAGLDA
                     QHLQLLVHSGS"
     gene            complement(758..1210)
                     /locus_tag="OO544_004253"
     CDS             complement(758..1210)
                     /locus_tag="OO544_004253"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR6769687.1"
                     /translation="MNNIQIRNYQPGDFQQLCAIFIRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVWVAVINAQPVGFISRIEHYIDMLFVDPEYTRRGVASALLKPFIK
                     SESELTVDASITAKPFFERYGFQTVKQQHVECRGAWFTNFYMQYKPQH"
     gene            complement(1207..>2109)
                     /gene="dinB"
                     /locus_tag="OO544_004254"
     CDS             complement(1207..>2109)
                     /gene="dinB"
                     /locus_tag="OO544_004254"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HCR6769688.1"
                     /translation="GVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTS
                     RIEPLSLDEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIAS
                     DMNKPNGQFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVML
                     LKRFGKFGRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPE
                     LERRLAKVKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGR
                     GVRLVGLHVTLLDPQMERQLVLGL"
BASE COUNT          531 a          522 c          523 g          533 t
ORIGIN      
        1 gagccgctat gaaccaacag ttgcagatgt tgtgcatcca gacctgccag tgcaaacagt
       61 tcagcgtcga tgatttgatc aacttgttgc agttcagcga aatgattgcc gccaccgata
      121 gagccaagcg agctgcgcca cggatgctgt gcaaatgctg acggcaggtt ttcctccagc
      181 cagctttctt cagcaacgtc atccaaagct gataatcgct tttcaaactt atcggcgttg
      241 tatttgcgag cgagaatatc tgtttgccat agcgccatgc cgcagccgat atcattgccg
      301 accagtgctg ggtaaaaacg gccaacagag aagaacgctg cgccaatcgg gtagccgcgt
      361 ccgggatgta aatctggcat ccccactacg cgctgcatgt tgggtaaatt tgccgtggtg
      421 tgtaattgtt ggatcgctaa actctcgatc cacaggtcat cagatgcgat ggtaaatacc
      481 gcatcggata agggacgaat atatttgccc atgtaccatt ccgtaaatag actcgtcata
      541 cttcaagttg catgtgctgc gactcgaatt attgagagta acaattaaaa aatcaggaaa
      601 tggcagacga atccagggca agaaaaaaag aattaatcct ggaaaggtct gcaaagagtc
      661 attgataaag gatttgtcat gatttacctc cttgcagttt gtggaataag aaaacgcgca
      721 catactaggc gggcgttatt ttcattgcaa gctggattta atgttgcggt ttatattgca
      781 tataaaaatt agtaaaccac gctccccggc attcaacgtg ctgctgctta actgtctgaa
      841 aaccataacg ttcaaaaaag ggttttgcgg ttatgctggc atctaccgta agttcggatt
      901 cagacttaat aaatggtttt aacaaggcgc tggcaacccc gcggcgggtg tattcagggt
      961 caacaaataa catatcgata taatgttcaa tgcgggaaat aaaaccaacc ggttgtgcat
     1021 taatgaccgc aacccacact tgtgatttcg cgagtttctc cttccagcga gattcgtcaa
     1081 tctgcgccca ggcggcaatt tgttgtggag aataatgctg actggcggtc atcgtaaccg
     1141 ctctaatgaa aatagcgcat agttgctgaa aatcgccagg ctgatagttt cttatttgta
     1201 tgttattcat aatcccagca ccagttgtct ttccatttgc ggatcaagca acgtcacatg
     1261 cagccccacc agtcgcacac cgcgcccgcc gcggcgttca tcccaggttt tacgcgcggt
     1321 ggcgattaag tcagctttat tcagccgcgg ccagacgtgc tcctgagtgg tttgctgaaa
     1381 atcgtcgaac tttaatttca ccccctggcg ggcaatcagt aaatcaggtt ttacctttgc
     1441 cagacggcgt tcaagttccg gatacagccg ctcgataatc gcttcacatt cagaccagtg
     1501 gtggatatct tccgccatcg tgcgttccac gccgacggat tttcgcaacc gttcgctgtt
     1561 aacgtcgcgc tcgtcaatcc cctgactacg ctcccacaaa atgcggccaa atttgccaaa
     1621 gcgtttaagc agcatcacca gatcacactt ttgtacatca ccgcaggttc gtagccccat
     1681 cgcttccagt tttgccgccg agactttgcc gacgccgggg atttttgcca gtggtaaggt
     1741 ttgtaaaaat gccggaactt ctgccggcgt aatcacaaac tggccgttgg gtttattcat
     1801 gtcggaggcg attttggcga gaaactttac gggtgccacg cccgcagacg ccgtcagttg
     1861 cagctcgttg aaaatcgtct ggcggatttc ctgggcgatg agggtcgcag aaccgtggca
     1921 atggacgcta tcggtgacgt cgagataagc ctcatccagt gacaacggtt caatacgcga
     1981 ggtgtagcgc gagaagattt cgcggatatg atttgaggct tctttgtagg cgtcaaagcg
     2041 ccccggaagc aaggtgagat gcgggcataa tttgagcgcc atccctgtcg gcatagcgct
     2101 acgtacgcc
//
LOCUS       DAJTZL010000332         2104 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.335, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000332 DAJTZL010000000
VERSION     DAJTZL010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.335"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            450..602
                     /gene="mokC"
                     /locus_tag="OO544_004255"
     CDS             450..602
                     /gene="mokC"
                     /locus_tag="OO544_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HCR6769689.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            complement(706..1836)
                     /gene="dnaJ"
                     /locus_tag="OO544_004256"
     CDS             complement(706..1836)
                     /gene="dnaJ"
                     /locus_tag="OO544_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HCR6769690.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(1925..>2104)
                     /locus_tag="OO544_004257"
     CDS             complement(1925..>2104)
                     /locus_tag="OO544_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6769691.1"
                     /translation="DKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKD
                     DDVVDAEFEEVKDKK"
BASE COUNT          499 a          555 c          513 g          537 t
ORIGIN      
        1 acacggaaag tcatatcagc gctaacgggt gaccctaagc cctgataatc atagcttaag
       61 gtcaggattt ctgagggatt aattaggaaa agtaataatt aatttcttgt cagtcggcgt
      121 tatttgttag gacaatattt caaaaataat ccagcttgtc gtcaataatt gcgataaata
      181 gataaattaa agttaaataa ttaaatcatt aaaaaattac gccacaaaaa taggcgaggc
      241 gtcttcagga ttagaatcga taaaacagaa tgggttaaag agagcgatat aatagcggcg
      301 ggtgcttgag gctatctgtc tcaggcatta gctgaacggc agatagagaa aagccccgag
      361 tgatatttta ctatcaaccc gaggccccct atatgctgaa cacatgtaga gtgcctctta
      421 ctgaccgtaa ggtcaaggag aagagagcaa tgaagcagca taaggcgatg attgtcgccc
      481 tgatcgtcat ctgtatcacc gccgtagtgg cggcgctggt aacgagaaaa gacctctgtg
      541 aggttcacat ccgaactggc cagacggagg ttgctgtttt cacggcttac gaatccgagt
      601 aagagcaacg gcggggagtg atccccgcca cttttcaggt gctcgcatat cttcaacgca
      661 ccctaatttt acccaagcct gcccacgggc aggcttttgg ggaggttagc gggtcaggtc
      721 gtcaaaaaac ttcttcacac catcaaagaa gctctttgag cgcgggctgt tgtgctcacc
      781 ggttgggcca ccgaagcttt cttgcagctc ttgcagcaac tgtttctgct tctcgttcag
      841 gcctaccggt gtttcgacga caacgcggca cagcaaatca ccctgtgcgc caccgcggac
      901 agacttgacg cctttaccgc gcatacggaa aagcttaccg gtctgggttt cgccaggcac
      961 tttcagtttg acgcgaccat caagggtcgg tacttcgatt tcgccaccca gcgccgccat
     1021 agcgaagttg atcgggactt cgcaatagag gttgttgcct tcacgctcga aaatcgggtg
     1081 ctgtttaacc tgaacctgaa cgtacagatc gcctgccggt gcgccatgct cgcccgcttc
     1141 accttcgccc gcaagacgga tgcggtctcc agtgtccacc cctgccggga ttttaacgga
     1201 cagcgttttg ctgcgctcaa cacgaccatg accatgacat ttgttgcacg gatctttgat
     1261 cagcgtaccg cggccctgac agtgtggaca ggtctgctgt acagcgaaaa atccctggcg
     1321 catctgcacc tgaccagaac catgacaggt cggacaggtc tgcggctgtg tacctggttt
     1381 tgcaccgcta ccgtggcaaa cgtcacactc ttccagagtc ggaatgcgga tctctttggt
     1441 cacgccacgt acagcttctt cgagggtgag ctccatgtta tagcgtaaat cagcaccgcg
     1501 cgccgcacgt tgacgaccac gtccgccgcc aaaaatatcg ccgaaaacgt caccaaaaat
     1561 atcgctgaag tctgcgccgc cgccaaaacc gccgccgccc atgccacctt gctcaaacgc
     1621 agcatgacca tactgatcgt atgccgcacg tttttgcgag tcggtcagaa cttcataagc
     1681 ttccttgatc tctttaaatt tcgcctcggc ctctttgtca ccctggttac ggtccgggtg
     1741 gtatttcatg gccaggcgtt tgtaggcctt tttgatttca cgctcttccg ctgttttgga
     1801 aacgcctaaa atctcgtaat aatcttgctt agccatcttt tttaaattgt ccctagatga
     1861 atgcacgggc ggagaggaaa ttccccttcg cccgtgtcag tataattacc cgtttatagg
     1921 gcgattattt tttgtctttg acttcttcaa attcagcgtc gacaacatcg tcatctttcg
     1981 cgttgtttgc agaagcatca gcaccggcag tctgctgctg ggcatgttgc tgctgggcga
     2041 tttccatcag tttctgggaa acctgtgcca gttcctgcat tttcgcttcg atagcggctt
     2101 tgtc
//
LOCUS       DAJTZL010000333         2101 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.336, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000333 DAJTZL010000000
VERSION     DAJTZL010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.336"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(60..1025)
                     /locus_tag="OO544_004258"
     CDS             complement(60..1025)
                     /locus_tag="OO544_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HCR6769692.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQTAAEFERYALRM
                     ASCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIV
                     VDYPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRG
                     KDGSAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGE
                     VVANVAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQL
                     DRETNEDLVIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
     gene            complement(1891..>2101)
                     /locus_tag="OO544_004259"
     CDS             complement(1891..>2101)
                     /locus_tag="OO544_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6769693.1"
                     /translation="RSEAVIPDRFKNLILLIDGLSFGTFGFHPHEGFEDELILYIQKT
                     NERVKTLFVKIDLNGIKFGFLRTHS"
BASE COUNT          582 a          551 c          439 g          529 t
ORIGIN      
        1 aaaaaaaata aaccaatagt cttatttcca ttcaggtatt caatattaat attcatacgt
       61 taatccgatt tatccttctc aggggcgcgt ttgtaacgcc gtttacctga atcccagaca
      121 aacgccgtcc gcttcccgtc atcagtgcca tcccctacat cgtcggcaat gacaagatcc
      181 tcattggttt ctcgatccaa ctggaggacg ttttttagcg ccccaccggt cgagataaaa
      241 cggcgcttgt gaagctgccg cgtcagctca agaaaccact caggatcgtt tgccatatct
      301 tccggtttaa cggagtattt cagcgtttca gcaaccgcgc tttgcagttg ctcggcaacg
      361 ttggccacaa cctcacctgt cttagttttt actgcccgaa tatcgatatt cggctcatag
      421 ttcacccgca agcaatcgcg ccagagttct acccaacgtt cgtgcttaac gtagttcttc
      481 cctttaaacc aagaaggttg caccatcagc agacagtgaa aatgcggatg tgcgctgcca
      541 tccttacctc gcgtcacctc cgtagccctg atccacccct gaacaggtga tagctccttt
      601 cgcttttcca ttcgcttaaa cgccgcattc attgctgtaa ggactgttcc aagttcacct
      661 atctcgcagt tcctgacagt taacgtcaga aacaaccatc gggaagacgg gtaatccaca
      721 acgattttcg gtagagcctg ataaaaacgg gcttgccaca tgagggttct tctccactgg
      781 cagacagggc aatgacggac acggcaaaat tgcgcactac gaagccgtag gcgcgtttct
      841 ccggtttcca tgatggtaga ccaaccaaaa cgtaacaaac cgctacagga ggccatacgg
      901 agtgcatagc gctcgaattc agcagcagtc tggtagatac ccccgacaga atcggtaagc
      961 gtgcgctggg tatcccaaac tgcatcacgg gggctgtagt ccgaaaggaa tttatcttct
     1021 gacattaagc ctgctccgat ctgggcaggc tgtgatttga tgtggtatat tcatcacgta
     1081 cctgtgcatt gcgagccagt acatgtcacc agagtttgcc gctgaggtga cacattcgaa
     1141 atgttgggga gtgttgacgc actccccaac gccgattaaa aacgcctcaa ttctgctcca
     1201 ttttctttga cctgtcaaaa cccactcaaa gcctcgtcat tactgggcta aaccactatt
     1261 tcatatccgc catttatccc ttaagtataa atacaaggat cacgcgcgag aattgcgcaa
     1321 aaaataacca caattttaac cccccaaaac aagcaccccc gaacggctta ccccaacctt
     1381 ttccacacac ccgatcacca aaacagggaa taaaaacaaa accctgtctt acaggtggat
     1441 ggcctcactt cgtgaaacca tccaccccgc ataagttttt taatcggtgg tgtcgtcaat
     1501 cactaaatcg gaaccccaaa ggggaccccc acgatttagc atttgacgag attaactctc
     1561 ggagatggca tcatgctttt aacggacttt aaaaaacggt gaacggatgc ggattttaat
     1621 ccgtgtctgt gaggcgttga gcaacgagca aagcgaggcg gtagaaagag agccagcggc
     1681 aggctcagga cgataatccg gggaaaatat accactcgca gagtgttaac atttgacatc
     1741 ggattgtcta tcctgcattg gtgtcgagtt ggcaaggctg aggataatgc tgaggataat
     1801 gctgaggata atgcaggata gacagagcac tagagattgc atgcaatctc gtacctatca
     1861 cctcagcctg ccgctggctc ttttccagaa ctaagaatgc gttcttaaaa atccaaactt
     1921 gataccgttt aaatctattt tcacaaaaag agtttttacc ctctcgtttg ttttctgaat
     1981 atataaaatt aattcatcct caaaaccttc gtgcggatga aaaccaaatg tgccaaaact
     2041 taatccatca ataagcaaaa taaggttttt aaacctgtca ggaatcaccg cttcacttct
     2101 t
//
LOCUS       DAJTZL010000334         2088 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.337, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000334 DAJTZL010000000
VERSION     DAJTZL010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.337"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            81..1253
                     /gene="istA"
                     /locus_tag="OO544_004260"
     CDS             81..1253
                     /gene="istA"
                     /locus_tag="OO544_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS21 family transposase"
                     /protein_id="HCR6769694.1"
                     /translation="MLSREDFYMIKQMRQQGAYIVDIATQIGCSERTVRRYLKYPEPP
                     ARKTRHKMVKLKPFMDYIDMRLAENVWNSEVIFAEIKAMGYTGGRSMLRYYIQPKRKM
                     RPSKRTVRFETQPGYQLQHDWGEVEVEVAGQRCKVNFAVNTLGFSRSFHVFAAPKQDA
                     EHTYESLVRAFRYFGGCVKTVLVDNQKAAVLKNNNGKVVFNSGFLLLADHYNFLPRAC
                     RPRRARTKGKVERMVKYLKENFFVRYRRFDSFTHVNQQLEQWIADVADKRELRQFKET
                     PEQRFALEQEHLQPLPDTDFDTSYFDIRHVSWDSYIEVGGNRYSVPEALCGQPVSIRI
                     SLDDELRIYSNEKLVASHRLCSASSGWQTVPEHHAPLWQQVSQVEHRPLSAYEELL"
     gene            1253..2050
                     /gene="istB"
                     /locus_tag="OO544_004261"
     CDS             1253..2050
                     /gene="istB"
                     /locus_tag="OO544_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS21 family helper ATPase
                     IstB"
                     /protein_id="HCR6769695.1"
                     /translation="MHELEVLLSRLKMEHLSYHVESLLEQAAKKELNYREFLCMALQQ
                     EWNGRHQRGMESRLKQARLPWVKTLEQFDFTFQPGIDRKVVRELAGLAFVERSENVIL
                     LGPPGVGKTHLAIALGVKAVDAGHRVLFMPLDRLIATLMKAKQENRLERQLQQLSYAR
                     VLILDEIGYLPMNREEASLFFRLLNRRYEKASIILTSNKGFADWGEMFGDHVLATAIL
                     DRLLHHSTTLNIKGESYRLKEKRKAGVLTKNTTPISDDEMVESGQHQ"
BASE COUNT          552 a          505 c          590 g          441 t
ORIGIN      
        1 acggtaattc gccatttgga ttgtccgctc cacccaacat gttgtttcct taaggttctc
       61 acaccagaaa ggacatcaac atgctgagca gagaggactt ttacatgata aagcaaatgc
      121 gccagcaggg cgcgtacatc gtcgatattg cgactcagat tggttgctct gaacggacgg
      181 tcagacgcta cctcaaatac cctgaaccgc cagccagaaa gacccgccac aaaatggtta
      241 agctgaaacc gtttatggat tacatcgaca tgcgcctggc agagaatgtc tggaatagcg
      301 aggttatctt cgcggagatt aaggcaatgg gttatacggg cggacgttcc atgctgcgtt
      361 actacatcca gcccaaacgt aaaatgcgtc cgtcaaaaag aacagttcgc ttcgaaactc
      421 agcctggata ccagctccag catgactggg gcgaagttga ggtggaggtt gccgggcaac
      481 ggtgcaaagt taactttgcg gttaatacgc tggggttctc ccgcagcttc catgtcttcg
      541 ccgcaccaaa acaggatgct gagcatacct acgaatcact ggttcgcgcc ttccgctact
      601 tcggtggttg tgtgaaaacg gtgctggttg ataaccagaa ggctgcggtg ctgaagaata
      661 acaacgggaa agtcgtgttc aactccggat tcctgttgct ggctgaccac tataacttcc
      721 tgccacgggc atgccgtcca cgcagggcca gaacaaaagg taaggttgag cggatggtga
      781 aatacctcaa ggagaacttc ttcgtccggt accgcaggtt cgacagcttc actcatgtca
      841 atcaacaact ggagcaatgg atagccgatg tggctgacaa acgggagctt cgccagttca
      901 aagaaacgcc ggaacagcgc ttcgcgctgg agcaggaaca tctgcagccg ttaccggata
      961 cagacttcga taccagttac ttcgatatcc gccatgtgtc ctgggacagc tatatcgagg
     1021 ttggtggtaa tcgttacagc gttcccgaag cgctgtgtgg tcagccggta tcgatacgaa
     1081 tatcgctgga tgacgagttg cggatctaca gtaatgagaa actggtggcc tcacatcgcc
     1141 tctgttcagc atcgtctggc tggcagacag tgccggagca tcacgccccg ctctggcagc
     1201 aggtcagtca ggtggaacat cgaccactga gtgcctatga ggagctgttg tgatgcatga
     1261 actggaagtc ctgctgagtc gcctgaaaat ggagcatctg agttatcacg ttgaaagcct
     1321 gctggaacag gcagctaaaa aagagctgaa ctaccgggag ttcctgtgca tggcgctaca
     1381 gcaggaatgg aacggcaggc atcagcgcgg tatggagtcc aggctgaagc aggctcgtct
     1441 gccgtgggtc aaaacgctgg agcagttcga ctttaccttc cagccgggca tcgaccgtaa
     1501 ggttgtccgg gaactggctg gtctggcgtt cgtggagcgc agcgaaaacg tgatcctgct
     1561 gggccctcct ggtgtcggaa aaactcatct ggccatagct cttggcgtga aagcggtgga
     1621 tgcgggacat cgggtactgt ttatgccact ggacagactg atcgcgacac tgatgaaagc
     1681 gaaacaggaa aaccggctgg agcgtcagct acagcaactg agttatgccc gggtgttgat
     1741 cctggatgaa ataggctatc tgccgatgaa cagagaggaa gccagtctgt tcttccggct
     1801 actgaaccgt cgatatgaaa aagcgagcat catactgacg tcaaacaaag ggttcgcaga
     1861 ctggggagaa atgttcggag atcacgtact ggcaacagcg atcctggatc ggctgctaca
     1921 tcactcaacc acgctgaata tcaaaggaga gagttaccgg ttaaaagaga aacgtaaagc
     1981 tggagtgctg accaaaaaca caacgccaat cagtgatgat gaaatggtgg aaagcggaca
     2041 gcatcagtaa cgaaagtatt tagcgggcat gaaaatggca aataacgg
//
LOCUS       DAJTZL010000335         2072 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.338, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000335 DAJTZL010000000
VERSION     DAJTZL010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.338"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(77..153)
                     /locus_tag="OO544_004262"
     tRNA            complement(77..153)
                     /locus_tag="OO544_004262"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(117..119),aa:Arg,seq:tct)
     gene            complement(167..243)
                     /locus_tag="OO544_004263"
     tRNA            complement(167..243)
                     /locus_tag="OO544_004263"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(207..209),aa:Arg,seq:tcg)
     gene            complement(251..326)
                     /locus_tag="OO544_004264"
     tRNA            complement(251..326)
                     /locus_tag="OO544_004264"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(291..293),aa:Ile,seq:cat)
     gene            complement(367..1425)
                     /locus_tag="OO544_004265"
     CDS             complement(367..1425)
                     /locus_tag="OO544_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HCR6769696.1"
                     /translation="MLNTVKISSCELINADCLEFIRSLPENSVDLIVTDPPYFKVKPE
                     GWDNQWKGDDDYLKWLDQCLAQFWRVLKPAGSLYLFCGHRLASDIEIMMRERFSVLNH
                     IIWAKPSGRWNGCNKESLRAYFPATERILFAEHYQGPYRPKDAGYAAKGSALKQHVMA
                     PLISYFRDARAALGITAKQIADATGKKNMVSHWFSAGQWQLPNESDYLKLQALFARVA
                     EEKHRRGELEKLHHQLVDTYTSLNRQYAELLSEYKHLRRYFGVTVQVPYTDVWTHKPV
                     QFYPGKHPCEKPAEMLQQIISASSRPGDLIADFFMGLGSTVKAALALGRRAIGVELET
                     ERFEQTVREVQDLVSQNG"
     gene            complement(1533..1773)
                     /locus_tag="OO544_004266"
                     /pseudo
     CDS             complement(1533..1773)
                     /locus_tag="OO544_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309113.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1998..>2072)
                     /locus_tag="OO544_004267"
     CDS             complement(1998..>2072)
                     /locus_tag="OO544_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCR6769697.1"
                     /translation="YDALVTRFDIEEAWAERQLKKVTR"
BASE COUNT          501 a          592 c          476 g          503 t
ORIGIN      
        1 cagacaactg tcaaccgtct gaattgtgag acatttaaaa aaaaggcccg caaaagcgag
       61 ccagggaaaa taagtgtggc gcgttgtact ggattcgaac cagtgaccga ttgcttagaa
      121 ggcaattgct ctgtccggct gagctaacaa cgcatgatgc agataatgga ccgccatcgg
      181 ggacttgaac cccgcgcagc cagcttcgaa ggctggcgct ctttcctgat gagctaatgg
      241 cggtatgtga tggtggccct tgctggattt gaaccagcga cctggcgatt atgagtcgct
      301 cgctctcacc actgagctaa agggccggga gccgcataat aacgacgcgt aattaattct
      361 tcaatatcat ccgttctggc tgactaaatc ctgtacttcc cgaaccgtct gctcaaaacg
      421 ttcagtctcc agctcaacgc caattgcacg acgcccgagc gccagtgccg ctttcactgt
      481 cgaacccaac cccatgaaaa aatctgcaat caggtcaccc ggacgactgc tagcgctgat
      541 tatctgctgc agcatttctg ccggtttttc gcacggatgt ttcccgggat agaactgcac
      601 cggtttatgc gtccacacat cggtatacgg cacctgcacc gtcacgccaa aataccgccg
      661 cagatgttta tattcactca gcagctccgc atactgccgg ttcagtgagg tatacgtatc
      721 caccagctgg tggtggagct tttccagttc accacgccga tgcttctctt ctgccacccg
      781 ggcaaacagc gcctgtaatt tcagataatc gctttcattc ggcaactgcc actgaccggc
      841 actgaaccag tgcgacacca tgtttttctt tcctgtggca tctgcaatct gttttgccgt
      901 tatccccagg gcagctcgcg catcacgaaa gtaagaaatc agcggggcca tcacatgctg
      961 tttcagtgca ctgcccttcg ccgcataccc ggcatctttc ggacgatacg gcccctgata
     1021 atgttccgcg aacagaatgc gctctgtggc ggggaaatac gcccgcaggc tttccttgtt
     1081 gcatccgttc cagcgtccgg acggcttcgc ccagataata tggttcagca cactgaagcg
     1141 ttcacgcatc atgatttcga tatcagatgc caggcgatga ccacagaaca ggtaaagact
     1201 tccggcaggt ttcagcaccc gccagaactg cgccagacac tggtccagcc acttcaggta
     1261 atcatcgtcg cccttccact ggttatccca gccctcaggc ttcactttaa agtacggcgg
     1321 gtccgtgact atcaggtcaa cagaattttc gggtaacgac cggataaatt ccaggcagtc
     1381 ggcgttgatt aactcacaac tggatatttt tacagtatta agcatggatc attaagcctg
     1441 tctctgatag gctcattctg cttttgcgca aagcagtggg cctgaggttt gcttgtgaac
     1501 ccaacgcatg agcagatggc tggtgggtgc ccctaacacc caccagccgc ccatttacca
     1561 caaataaaaa agccttcact gcggaaggcg tctgtaacaa ccgaactgat agtctgccag
     1621 acccgccata accagctggg tcagtattaa ctggcagcgt tcgcgtgaaa ggtaagtatt
     1681 ctgcgctatc tccccgactg tcgccggttc ggtaacgctt aattcattaa acaccactct
     1741 ggcggtttct gtcatatcct gctgttttag catgtctttt ccttttccgg ttaacgtgac
     1801 acaccaataa ctcttgtcga aaaagccagc aagctgaaag acaggtattc accgccacca
     1861 gcgcgtttac tgtactgacg cgatttcagt cataaaaaac ccgctcggcg gcgggttgta
     1921 gaaactcttc taacgtcagg cataaaacgc ccatcgttat gacgaattta ccacagattc
     1981 cggaaaaatc aaccttgtta cctagttacc ttttttaact gccgctcagc ccatgcttct
     2041 tcaatatcaa accgggtcac cagcgcatca ta
//
LOCUS       DAJTZL010000336         2050 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.339, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000336 DAJTZL010000000
VERSION     DAJTZL010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.339"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..675
                     /locus_tag="OO544_004268"
                     /pseudo
     CDS             1..675
                     /locus_tag="OO544_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309971.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
     gene            682..1428
                     /locus_tag="OO544_004269"
     CDS             682..1428
                     /locus_tag="OO544_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR6769698.1"
                     /translation="MKNIATGGVLERIRRLTPPHVTAPFRTVAEWREWQLAEGQKRSE
                     EINRLNRQLRVEKILNRSGIQPLHRKCSFANYQVQNDGQRYALSQAKSIADELMTGCT
                     NFAFSGKPGTGKNHLAAAIGNRLLKDGQTVIVVTVADVMSALHASYDDGQSGEKFLRE
                     LCEVDLLVLDEIGIQRETKNEQVVLHQIVDRRTASMRSVGMLTNLNYEVMKTLLGERV
                     MDRMVMNGGRWVNFNWESWRPNVSHSRVVK"
     gene            1443..1865
                     /locus_tag="OO544_004270"
     CDS             1443..1865
                     /locus_tag="OO544_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HCR6769699.1"
                     /translation="MAKPFTPEQREELKTRIVELVHQDGRVTIRQLSDETGISRASVG
                     RLCIELVASGDVYNSGYGLFPSEQARKDWQSARKKLSRVKVRKPVVVDPDLIWSLPDG
                     EIRRYDRRLNIICRECRKSEAMQRVLAFYQGNFQEAIL"
     gene            1866..>2050
                     /locus_tag="OO544_004271"
     CDS             1866..>2050
                     /locus_tag="OO544_004271"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6769700.1"
                     /translation="MKLAIRLQLPLAFASKERSMEIKPEDELSNIVLFPVKEDDPRNQ
                     VNFLYEPSERPYCHHASV"
BASE COUNT          556 a          456 c          592 g          446 t
ORIGIN      
        1 ttattcgctt ctgggcatgg gcggatcaac aaatgataga cggtaatgca gattgtaacg
       61 ctcgcggcgt tacaaaaagt gcaatagatc gcatcacttt tatgtctggt tttgctgatg
      121 cgttaattca ggttggatgg ctggtcgaaa atgacggagg gctttctcta cctaactttg
      181 aacgtcataa cggaaaaagc tctaaaaaaa acgggcggtt acaaacgagc gagtaacaaa
      241 aatacgcgaa ctgaaacgaa aaggtaacgc tgccagcgtt acacaaacgg atcaaaaagc
      301 gttaccagag gaagaggaag aggaagatct aaatactgat ctccccctcg ccaaaaacga
      361 gcgtctaaaa aattcgagcc ggaggctatt gagctgcccg attggttgcc ggaaacactc
      421 tggcatgagt gggtccggtt cagacaggca ttgcgaaaac cgattcgaac ggagcagggc
      481 gctaacgggg cgatacggga actggaaaaa ttccgtcagc agggttttac acctgagcag
      541 gtgattcgac acagcatcgc caatgaatac cagggcctgt tcgcgccgaa aggtgttcgg
      601 cctgagacgt tgctccgaca ggttaacacc gtctcgttgc cggacagtgc gatcccgcca
      661 ggcttcaggg ggtaacggac catgaaaaat attgcgacag gcggcgttct ggaacgcatc
      721 cgccgactga ccccgccaca tgtaaccgcc ccattcagaa cggtagcgga gtggcgcgag
      781 tggcaacttg ctgaaggcca gaaacgtagc gaggagatca accgcctgaa tcgccagttg
      841 cgggtggaaa aaattctgaa tcgctcaggc atccagccgt tgcaccgtaa atgctcgttt
      901 gcgaattacc aggtgcagaa cgacggccag cgatacgcgt taagccaggc gaaatccatc
      961 gccgatgaac tgatgaccgg gtgtacaaat tttgcgttca gcggaaaacc tggtaccggg
     1021 aagaatcact tagcggcagc tatcgggaat cgcctgctga aagacggtca gacagtgatt
     1081 gtggttaccg tggctgatgt tatgagcgcc ctgcacgcca gctatgacga cgggcagtca
     1141 ggcgaaaaat ttttgcggga gctgtgcgaa gtggatctgc tggttcttga tgaaattggc
     1201 attcagcgcg agacgaaaaa cgagcaggtg gtgctgcacc agattgttga tcgccggaca
     1261 gcgtcgatgc gcagcgtggg gatgctgaca aacctgaact atgaggtgat gaaaacattg
     1321 ctcggcgagc gggtgatgga tcgcatggtc atgaacggcg ggcgctgggt gaattttaac
     1381 tgggagagct ggcgtccgaa tgttagccat tcgagggttg ttaagtagtt tcaggaggat
     1441 ttatggcgaa accttttact cccgaacagc gggaagaact gaagacgcga attgtggaac
     1501 tcgtgcatca ggacggtcgg gtcacgattc ggcagttgtc agatgaaaca ggtatcagtc
     1561 gtgcgtctgt cggtcgctta tgcatagaac tggtcgcaag tggtgatgta tataattctg
     1621 gctacggctt attcccgtct gaacaggctc gcaaggactg gcaaagcgcc cgcaaaaaac
     1681 tctcgagagt aaaggtgagg aaaccggttg ttgttgatcc ggaccttatc tggtcattac
     1741 ctgacggaga aatacgccgc tacgacaggc gcctgaatat aatctgtcgc gagtgccgga
     1801 agagcgaagc tatgcagcgt gtactggctt tctatcaggg taattttcag gaggcgatac
     1861 tgtgaatgaa attagctatc aggcttcaat taccgctggc attcgcatca aaggagagga
     1921 gcatggaaat aaaaccagaa gatgagttaa gcaatatcgt tttatttccg gtaaaagagg
     1981 atgaccctcg taatcaggtt aattttcttt atgagccatc ggaaagacca tattgccatc
     2041 acgcctctgt
//
LOCUS       DAJTZL010000337         2024 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.340, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000337 DAJTZL010000000
VERSION     DAJTZL010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.340"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..305)
                     /locus_tag="OO544_004272"
     CDS             complement(<1..305)
                     /locus_tag="OO544_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HCR6769701.1"
                     /translation="MAHIQLVKQTSSGLLLPATPESCDFLHQIKIGEWIHADFKRVCN
                     YAFHKRFFKLLQLGFDYWTPVGGAITPRERKLVSGFVDYLCESVGREHTPALSEAAE"
     gene            complement(344..631)
                     /locus_tag="OO544_004273"
     CDS             complement(344..631)
                     /locus_tag="OO544_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6769702.1"
                     /translation="MKIEELREIFSENGLYAVRVENGAIVSHCRIRCLQSQQRKSGAV
                     LFYFCNGLLTDGFILREDEFVTSLRVLKEIGFKAGFLLLLKNKLIYNLEQD"
     gene            complement(881..1036)
                     /gene="hokD"
                     /locus_tag="OO544_004274"
     CDS             complement(881..1036)
                     /gene="hokD"
                     /locus_tag="OO544_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HCR6769703.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     VDYESRK"
     gene            complement(1264..1929)
                     /locus_tag="OO544_004275"
     CDS             complement(1264..1929)
                     /locus_tag="OO544_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HCR6769704.1"
                     /translation="MTVADFKRPKLELPNGANKLLLHSCCAPCSGEVMEALQASGIDY
                     TIFFYNPNIHPQKEYLIRKDENIRFAEQHGVPFIDADYDTDNWFERAKGMEWEPERGI
                     RCTMCFDMRFERTALYAAENGFSVISSSLGISRWKNMQQVNDCGRRAVAHYPGMVYWD
                     YNWRKQGGSSRMIEISKREKFYQQEYCGCVYSLRDTNLHRKSQGRPLIKIGQLHYGKE
                     EKE"
BASE COUNT          516 a          524 c          459 g          525 t
ORIGIN      
        1 tgctctgcgg cttcgctcag agctggcgta tgttcccggc ctactgattc acacaggtaa
       61 tcaacgaaac cggacaccag ttttcgttcg cgaggcgtga tcgccccacc gaccggagtc
      121 cagtaatcga aacccagttg caggagtttg aaaaaacgct tgtggaatgc gtagttacac
      181 acacgcttaa agtctgcgtg tatccactca cctattttga tttgatgcag aaaatcgcaa
      241 ctctccggcg tcgccgggag aagtaatccg gaagaggttt gtttgaccag ttgtatatgt
      301 gccatcggct ttctccggtg gcacggtgtt acacagcagg agttcaatcc tgctcaagat
      361 tgtagatgag tttattcttc agcaaaagca gaaaaccagc cttaaaacca atctctttca
      421 aaacccgtaa tgatgtgaca aattcgtcct cacgcaaaat aaaaccgtcc gtcagaagtc
      481 cattacaaaa ataaaataac acagcaccgc tcttcctttg ttgagattgc aaacatctaa
      541 tgcggcaatg gctgacaatc gctccattct caacgcgcac agcatagagg ccattttcac
      601 taaaaatttc acgcaattct tcgattttca tcttcagaat ccttccagat aaatagctct
      661 cccctgttcg gggtccatcc ctcttctccc tgcgcgctac ttaagtgagt cgattctatc
      721 tgcgaaggtg cgcgaatcaa attcaccgga aataaacaac aaaaaacccg ccgaaacgct
      781 gagagatcac agcgatgggc gggttaagtg cgggtgcgtt gaggatgcct gacacatcag
      841 aggtgggcgg ggatgggatc agctccccgc ccggtcactc ttacttcctg gattcgtagt
      901 ctacgaagac agcgacctcc gtctggccgg ttcggattcg tacctcacag aggtctttcc
      961 tcgttaccag tgccgtcact atgacggtta aacagatgac gatcagggcg attaacatcg
     1021 ccttttgctg cttcatagcc tgcttctcct tgcctttcgg catgtaagag gctaacctac
     1081 atgtgcaaag catgaaattg gcctcagatt aatgttaagc gtcttgccgg acgcgtaatg
     1141 ttaactgggg cttttctcta tctgcctttt ggtgttcatg cctgaagcag atagcctcaa
     1201 gcacccgcag tcattctact tacctcgcca atatgaaatc aatcagaaag gtgccccata
     1261 aaatcactcc ttctcttctt taccgtagtg gagttgacca attttgataa gagggcgtcc
     1321 ctgagatttg cggtgtagat tggtatcgcg cagagaatac acacagccac aatattcctg
     1381 ctgatagaat ttttcgcgct tgctgatttc aatcatacgg gacgagccgc cctgcttgcg
     1441 ccagttataa tcccagtaca ccatgcccgg ataatgcgcg acggctcgcc gcccacagtc
     1501 gttaacctgc tgcatatttt tccagcgtga aatgcccagt gaactgctga tcacactgaa
     1561 accattttca gcggcgtaca gcgctgtccg ctcaaaacgc atgtcaaaac acatggtgca
     1621 acggatccct cgttcgggct cccattccat tcctttggct cgttcaaacc agttgtctgt
     1681 gtcgtaatca gcatcaataa acggcacgcc gtgttgttca gcaaagcgaa tattctcatc
     1741 cttacgaatt aaatactctt tctgaggatg aatgttcggg ttgtagaaaa agatggtgta
     1801 gtcgattccc gaggcctgaa gcgcctccat cacctcaccg gaacatggag cacagcaaga
     1861 gtgcagtagt agtttgtttg ccccgtttgg gagctccaat ttgggccgtt tgaaatcagc
     1921 aactgtcata aatatgttta ttggggtcat aaaaatatca aagagtgtag cattagaacg
     1981 gggctatcgg aaacagatgt gtgactgctc cccgcccttt cggg
//
LOCUS       DAJTZL010000338         2013 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.341, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000338 DAJTZL010000000
VERSION     DAJTZL010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2013
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.341"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(37..273)
                     /locus_tag="OO544_004276"
     CDS             complement(37..273)
                     /locus_tag="OO544_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HCR6769705.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYVLLPSCLALIAAIMLMIMNLNQG"
     gene            414..1202
                     /gene="fhuF"
                     /locus_tag="OO544_004277"
     CDS             414..1202
                     /gene="fhuF"
                     /locus_tag="OO544_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HCR6769706.1"
                     /translation="MAYRSAPLYEDIIWRTHLQPQDAGLAQAVRATIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQLYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            complement(1240..1917)
                     /gene="bglJ"
                     /locus_tag="OO544_004278"
     CDS             complement(1240..1917)
                     /gene="bglJ"
                     /locus_tag="OO544_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HCR6769707.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHPY"
     gene            complement(1875..>2013)
                     /locus_tag="OO544_004279"
     CDS             complement(1875..>2013)
                     /locus_tag="OO544_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HCR6769708.1"
                     /translation="AIHSNAEMYAWINSAQGARELNLPSVYGDAAEWNTAELRREMSH
                     S"
BASE COUNT          527 a          530 c          439 g          517 t
ORIGIN      
        1 tatctttact tcctttcttg tttcttcctt gatttttcat ccctgattca aattcattat
       61 catcagcatg atggcagcaa taagcgccag gcacgatggt agcaaaacgt aatgccgtaa
      121 ctgtttgtga tacagcatca ccggcgtcaa tactaatccc atcagaatga caatccgcca
      181 ctgatccggc gataaatccg catacatcag ccctgcaatc agcaatcccg gcagcaacac
      241 tccccagcca ctgcccagcg tacgttgcaa catggtttca tctctttcat tgataatgat
      301 aaccaatatc atatgataat ttttatcatt tgcaagccag agaaatccct tgctatcggg
      361 taaacctatc gctaggatta gcaatcatta tcatttagat tactatcccg attatggcct
      421 atcgttccgc accgctctat gaagatatca tctggcgaac gcatctccag ccgcaggatg
      481 ctgggcttgc acaagccgta cgtgcgacga tcgccaaaca tcgtgaacat ttgctcgagt
      541 ttatccgcct ggatgaaccc gctccgctta acgccatgac gctggcgcaa tggtcatcac
      601 cgaatgcgct aagttctcta ctcgcggtct attccgatca tatctatcgc aaccaaccaa
      661 cgatgatccg cgagaacaaa ccgctgatct cactatgggc acaattgtat atcggcctga
      721 tggtgccacc attaatgctg gcgctactga cgcaggaaaa ggcattagat gtgtcgccgg
      781 aacatttcca tgctgagttt cacgaaaccg gacgcgtcgc ctgtttctgg gtcgatgtgt
      841 gtgaagataa aaacgcaaca ccacattcgc cgcagcagcg aatggaaacg ttaatcagcc
      901 aggcgctggt tccggttgtg caggcactag aagcgaccgg agagatcaac ggcaaactta
      961 tctggagtaa taccggttat ttgatcaact ggtatctcac tgagatgaag caactgctcg
     1021 gcgaggcgac ggttgaatcg ctgcgccatg ccctcttttt tgagaaaacg ctcacgaacg
     1081 gtgaagataa tccactctgg cgtaccgtcg tgctgcgcga tggcctgctg gtgcgccgaa
     1141 cctgctgcca gcgttatcgc ttaccggatg tgcagcaatg tggcgattgt acgctgaaat
     1201 gaaagcactg ccggggaagt aaacccggca tcatgcggat taatagggat gcaacacatt
     1261 acttgcttcg caatgccgca tacataacag aatatctgcg gcctccaaca accctgcatc
     1321 agaactgacg cccagtttcg acatcacatt aaatttatgt gcacggatcg ttttgatatt
     1381 gcgtttaagc tgctcggcaa tttgtgtcat tgagtagcca cgcgacataa agcgcaatat
     1441 ttctctctcc gtcgggctta gcgtccggct ttggttaatg taccactgat tgttcagtcg
     1501 gtcggtcgcc tgacgtacac catttaatga caaaaagagt tcctgatgaa aaatctccag
     1561 cgttgacgct ttacttaata caccgtccag cggtgatggc gacaatgaac caatcagccg
     1621 agcttcaata tcatcatccg caataactaa acgccgggta cgcggaaact taatcgccag
     1681 ttcagtcagg caagataatc cctctctgcg ctcacttctc atggcagaaa aagaaaaaat
     1741 gaccgccgca aatgagatcc gcgacatggc atcctgaaat gactcctgac tggtataggt
     1801 atggagctta taagggttac ccgcaaactt tctgaataaa ctctcaatac cgatactgct
     1861 catgacgcat ttttctatga gtgcgacatt tctcttctta attcggctgt gttccattct
     1921 gcggcatctc cataaacaga aggcaagtta agttctcttg caccctgcgc gctatttatc
     1981 catgcataca tttcagcgtt gctgtgaatt gcc
//
LOCUS       DAJTZL010000339         1965 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.342, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000339 DAJTZL010000000
VERSION     DAJTZL010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1965
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.342"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..264
                     /locus_tag="OO544_004280"
     CDS             <1..264
                     /locus_tag="OO544_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HCR6769709.1"
                     /translation="AEKFATAIRNHWHVENKLHWRLDVVMNEDDCKIRRGNAAELFSG
                     IRHIAINILTNDKVFKAGLRRKMRKAAMDRNYLASVLAGSGLS"
     gene            434..712
                     /locus_tag="OO544_004281"
     CDS             434..712
                     /locus_tag="OO544_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
                     /protein_id="HCR6769710.1"
                     /translation="MGKPDVTDSNLKNIIDDLYRPNAKVGSGSTADAVRYELATGEKV
                     GGRGHVLKAQDYTRALQDWLDKNPTASPGDRAAAENVLQDLQNALGGK"
     gene            712..1089
                     /locus_tag="OO544_004282"
     CDS             712..1089
                     /locus_tag="OO544_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6769711.1"
                     /translation="MNQYIASYIEKMASFSTVLSETVSLANEYWHPETPPVVMLFSQV
                     GKTIVKNFYEMGDIEREKIFKHVEDGMVSLDDELATVVATGLIEAIVTATDSNSLLWQ
                     DLSKFLGDKSKSHALVWKNFGQE"
     gene            1558..>1965
                     /locus_tag="OO544_004283"
     CDS             1558..>1965
                     /locus_tag="OO544_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HCR6769712.1"
                     /translation="MELKKLMEHISIIPDYRQAWKVEHKLSDILLLTICAVISGAEGW
                     EDIEDFGETHLDFLKQYGDFENGIPVHDTIARVVSCISPAKFHECFINWMRDCHSSDD
                     KDVIAIDGKTLRHSYDKSRRKGAIHVISAFSTMH"
BASE COUNT          640 a          301 c          470 g          554 t
ORIGIN      
        1 gcagaaaagt tcgccacagc aatccgaaac cactggcacg tggagaataa gctgcactgg
       61 cgtctggacg tggtaatgaa tgaagacgac tgcaaaataa gaagaggaaa cgccgcagaa
      121 ttattttcag ggatacggca catcgctatt aatattttaa cgaatgataa ggtattcaag
      181 gcagggttaa gacgtaagat gcgaaaagca gccatggata gaaactatct cgcgtcagtc
      241 cttgcgggga gcgggctttc gtaatctttc cctagggagt cagccgaatg ttgggaagga
      301 tttgacggat gaagagaaga actgcttggc cggcggcagt acgggaagcc cgggaggatg
      361 gggaccagaa gatgagaata gcgcaaggga tcgtatgcag catgaacggt ataaagatga
      421 attgcgacag aaaatgggaa aacccgacgt tacagattca aatttgaaaa atattataga
      481 cgatctttat cggccaaatg caaaagtagg aagtggaagc acagcagatg ctgttcgtta
      541 tgaattggcc acaggtgaaa aagttggtgg tagaggacat gtattgaaag cacaggatta
      601 tacaagggct ttacaggatt ggttggataa gaatccaaca gcatctccag gggatcgagc
      661 agcagcagaa aacgttcttc aggatttaca gaatgctttg ggaggcaagt aatgaaccaa
      721 tatattgcta gttatataga aaaaatggca tcattttcca ctgttttgtc tgagacagtc
      781 tcattagcta atgaatactg gcatccagaa acgccaccgg tggttatgtt gttctctcag
      841 gttggaaaaa cgattgtaaa aaatttttat gaaatggggg atattgaaag agaaaaaata
      901 tttaaacatg tcgaagatgg tatggtctcg cttgatgatg aattggccac agttgtagct
      961 acagggctta tagaggcaat agtcactgcg accgatagca attctttact ttggcaagat
     1021 ttaagtaaat ttctgggtga caaatcaaag tctcatgcat tagtgtggaa aaattttggt
     1081 caggagtgat attattcaag aagtattaag ctcgctattg agatcttaat gcttcttgag
     1141 attaaattaa agttaggacg aagtaattgc aggtggaaat aaatccatag tgacgtgtgg
     1201 caatggctat tagatgttat ggtgtgatgg gctttggtga tttaagagct aagttagatt
     1261 gccttatgaa aaagtatggt tatatattta atgatgtgta tcatatattt aaataaaaaa
     1321 cggttctctc ttgtcaagat agtaatagga tagactattg tgttgataaa tataattaag
     1381 agtctacttt gttgatttta gaagactttg gagcagggaa agatcatcgg aagtacactt
     1441 tttgtactaa ataattcgcg ctaaatgttc aataattgag atattcctta ttacctaaaa
     1501 ctatttttca tagcttatat atgatcaaat actccttgca taaataagga gaacaaaatg
     1561 gaacttaaaa aactgatgga acatatttct attatccccg attacagaca agcctggaaa
     1621 gtagaacata aattgtcaga tattctactg ttgactattt gtgccgtcat ttctggtgca
     1681 gagggttggg aagatataga ggattttggt gaaacacatc tcgatttttt gaagcaatat
     1741 ggtgattttg aaaatggtat tcctgttcac gatactattg ccagagttgt atcctgtatc
     1801 agtcctgcaa aatttcacga gtgctttatt aactggatgc gtgactgcca ttcttcagat
     1861 gataaagacg tcattgcaat tgatggaaaa acgctccggc actcttatga caagagtcgc
     1921 cgcaagggag cgattcatgt cattagtgcg ttctcaacaa tgcac
//
LOCUS       DAJTZL010000340         1899 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.343, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000340 DAJTZL010000000
VERSION     DAJTZL010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1899
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.343"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(89..643)
                     /locus_tag="OO544_004284"
     CDS             complement(89..643)
                     /locus_tag="OO544_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HCR6769713.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYIGGGKTGN
                     MFNLMLTSKKLTKTAINEALRRMKKAGLNKSELEAFLRDMINGKQKSWLAHCTDAEAL
                     CIDRVISEVLAEHPGLISVLRQRYEGRGMTKRKMAELLNDAHPKWSLRTCERRIEHWL
                     KVAEFILYKPMVMAFGIEKKVIAF"
     gene            complement(640..930)
                     /locus_tag="OO544_004285"
     CDS             complement(640..930)
                     /locus_tag="OO544_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HCR6769714.1"
                     /translation="MVDLRKAARGQMCTVRIPGYCNHDPETSVLAHYRLAGTCGTAIK
                     PHDMQAAIACSSCHDLIDGRVKTSDYTKEELRLMHAEGVFRTQEIWRKEGYL"
     gene            complement(930..1529)
                     /locus_tag="OO544_004286"
     CDS             complement(930..1529)
                     /locus_tag="OO544_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HCR6769715.1"
                     /translation="MAHIQLIKQTSSGLLLPATPESCDFLHQIKTGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFDYWTPVGGAITPRERELLSGFVDYLCESVGREHTPALSEAAEQ
                     YLNTVATRRTRDTALLKSFEAFREWVTIQAGFYTEHFYPDGSRGRRAKSIAFANMDET
                     EFQQVYKSVLNVLWNWILFRKFSSPEQVENVAAQLLEFA"
     gene            complement(1601..1813)
                     /locus_tag="OO544_004287"
     CDS             complement(1601..1813)
                     /locus_tag="OO544_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6769716.1"
                     /translation="MEKIKKLFSSKYAVIRRDDLSVIVEMDYFPETKKSMMYRNGRKA
                     IFLPMRVSDIMGNDKLLDELRVRASC"
BASE COUNT          472 a          501 c          387 g          539 t
ORIGIN      
        1 taaagcgggc tctttcaaat gtccatgtct gctattcgcc tcgcggtaca gctttgcgaa
       61 gcgtacaaga attgaagcag tttttacgtc aaaaagcaat aacttttttc tctataccaa
      121 aagccataac cattggtttg tacaaaataa attctgccac ctttagccaa tgctcaatgc
      181 gtctttcaca ggttcttaaa ctccatttcg ggtgtgcatc attcagcagt tcagccattt
      241 tgcgtttggt catcccccgc ccctcatacc gttgccgaag gacgctaatc aatcctggat
      301 gctctgccag cacctcactt atgacccgat caatacataa cgcctctgca tcagtacaat
      361 gcgccagcca gctcttttgc ttaccgttaa tcatatcccg caaaaaagcc tcaagttcag
      421 acttgttcag acctgctttt ttcatcctcc ggagcgcctc gttaattgcc gtttttgtca
      481 gctttttaga ggtcaacatc aggttaaaca tattccccgt cttaccgcca ccaatatacg
      541 accagcgccc ccacatacgt agttttccct gaatccagac actttccagc gtggtgagac
      601 gaaggtgttc tccgcttttt cctgtattcg ttgggtaaat cataaatatc cctcctttct
      661 ccagatttct tgtgtgcgaa aaacaccttc tgcatgcatc aggcgtaatt cttctttggt
      721 gtaatcgctg gtttttaccc gcccgtcgat taaatcgtgg cacgagctac aggcaatcgc
      781 tgcctgcata tcgtgtggct ttatcgctgt tccgcacgtt cccgccagtc ggtaatgcgc
      841 cagcacagac gtttccggat cgtgattgca gtagccagga attctgacgg tgcacatctg
      901 cccccgcgcc gctttacgta aatccaccat tacgcaaact ccagcagctg cgcggccaca
      961 ttttcgactt gttccggaga ggaaaattta cggaacagaa tccagttcca cagcacattc
     1021 agaacagatt tataaacctg ctgaaactcg gtttcgtcca tattcgcaaa agcgatggat
     1081 tttgcccgac gcccgcggct accgtccgga taaaaatgtt cggtgtaaaa tccagcctga
     1141 atggttaccc actcgcgaaa agcctcaaac gactttagca atgccgtatc ccgggttctg
     1201 cgtgtcgcaa ctgtattcag atattgctct gcggcttcgc tcagagctgg cgtatgttcc
     1261 cgacctactg attcgcacag gtaatcaacg aaaccagaca gcagttctcg ttcgcgaggc
     1321 gtgatcgccc caccgaccgg agtccagtaa tcgaatccca gttgcaggag tttgaaaaaa
     1381 cgcttgtgga atgcgtagtt acgcacacgc ttaaagtctg cgtgtatcca ctcacctgtt
     1441 ttgatttgat gcagaaaatc gcaactctcc ggcgtcgccg ggagaagtaa tccggaagag
     1501 gtttgtttga tcagttgtat atgcgccatc gtagttctcc gctggcgcag tagaatgggt
     1561 gttcagcccg ttatgtagta taccagaatt aatgccaata ctaacaggat gctctgactc
     1621 gcaattcatc cagcagttta tcatttccca taatgtcact taccctcatc ggtaaaaaaa
     1681 ttgcctttcg accattacga tacatcattg attttttggt ttcagggaag taatccattt
     1741 cgactataac tgacaggtca tcacgacgta tgactgcgta tttactacta aatagtttct
     1801 ttattttttc cacgatgcct ccaggtttat aagtacaaac ggttatatca acatagagac
     1861 aaaaggtgat gctgccaact tactgattta gtgtatgat
//
LOCUS       DAJTZL010000341         1887 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.344, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000341 DAJTZL010000000
VERSION     DAJTZL010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1887
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.344"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..389
                     /locus_tag="OO544_004288"
     CDS             <1..389
                     /locus_tag="OO544_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6769717.1"
                     /translation="FDLSLEIPLPPPGILSKTVVPGESSATVKQRVMAARERQFKRQN
                     KLNAWLDSPEIRQFCKLESEDAMWLEGTLIHLGLSIRAWQRLLKVARTIADIDQSDII
                     TRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(414..752)
                     /gene="maoP"
                     /locus_tag="OO544_004289"
     CDS             complement(414..752)
                     /gene="maoP"
                     /locus_tag="OO544_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HCR6769718.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            871..1710
                     /gene="hdfR"
                     /locus_tag="OO544_004290"
     CDS             871..1710
                     /gene="hdfR"
                     /locus_tag="OO544_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HCR6769719.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(1806..1881)
                     /locus_tag="OO544_004291"
     tRNA            complement(1806..1881)
                     /locus_tag="OO544_004291"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1846..1848),aa:Trp,seq:cca)
BASE COUNT          503 a          446 c          457 g          481 t
ORIGIN      
        1 gcttcgatct ctcactggag atcccattac caccccccgg cattttgagt aaaacggtag
       61 tgccgggaga aagcagcgcc accgttaaac aacgcgtaat ggccgccaga gagcgccaat
      121 ttaagcggca gaataaactg aatgcctggc tggatagtcc ggaaatacgc caattctgca
      181 aacttgagag cgaagatgcg atgtggttgg aaggaacact gatccatctg gggttatcga
      241 ttcgtgcctg gcagcggtta ttgaaagttg cacgaaccat tgctgatatt gatcagtctg
      301 acattatcac acgtcagcat ttgcaggagg cagttagcta tcgtgcgatt gaccgtttgc
      361 tcatccatct gcaaaaacta ctgacataaa aaaagggcat ttcgcccttt ttattaatca
      421 tcagaatcgg tgtagtcttc agcaccttca acctgcggtt taccgccgga aagggtgtga
      481 aaacgttttg gacgcttaat acgcgtcata tacttggacc acacgcgttc tgcttctgtc
      541 actggctcac gttcgccacg gcatactgct acgaagagtt tctcttcctc ggtaaccggc
      601 tcgcgtttgc caagatccaa ctcattgaag gcataaccat gacgctcaag cagttgtgcc
      661 tctttgatgg tgaaatcacc atgacgagag aatccacgtg gataatgttt attgtcgaaa
      721 tatcgattag tcgtcgtaaa gctttccgcc atcctgcacg ctcctaattc tttgaccgag
      781 ctagttatgg cgcggagtat tagttacgct tgacagagtg taaaacaaaa catttaaatc
      841 ataacgacaa ataattttgc ggagagcact gtggatacgg aattgttaaa aactttcctg
      901 gaagttagcc gaacgcgtca ctttggtcga gcggctgaat cgctctatct gacccagtca
      961 gcagtgagct ttcgaatcag gcaactggaa aatcaactgg gtgtgaacct tttcacccgc
     1021 cacagaaaca atatccgttt aaccgctgcc ggtgaaaaac tactgcctta tgcagaaacg
     1081 ctcatgagca cctggcaggc cgcccgcaag gaggtggcgc atacctcacg acataacgag
     1141 ttttctatcg gtgccagcgc ctcgttgtgg gaatgtatgc ttaatcagtg gttgggacgc
     1201 ttgtatcaaa atcaggatgc ccatacaggc ttacagttcg aagcgcgaat tgcccaacgg
     1261 cagtctctgg taaaacagct gcatgaacgc cagcttgatc ttcttattac cactgaagcg
     1321 cccaaaatgg acgaatttag tagtcagttg ctggggtatt tcactttagc gctttatacc
     1381 agtgcccctt ctaaactaaa gggagatctt aactatctgc gactggagtg ggggccagat
     1441 tttcaacagc atgaggcagg tttgatcggt gctgacgaag ttcccattct gacaaccagt
     1501 tctgctgaac tggcacagca acagattgcg atgcttaatg gttgcacctg gctacccgtt
     1561 agctgggcgc gtaaaaaagg cggcctgcat accgttgtcg atagcacaac actttcacgg
     1621 ccgctttatg ccatatggct gcaaaatagc gataaaaatg cgttgattcg cgatcttttg
     1681 aaaattaacg tgctggatga agtgtattaa tctgaatggc tggcaaggat gccggtagaa
     1741 ggatttactt cggagaggat tatttcagat aaaaaaaatc cttagctttc gctaaggatg
     1801 atttctggca ggggcggaga gactcgaact cccaacaccc ggttttggag accggtgctc
     1861 taccaattga actacgcccc taattag
//
LOCUS       DAJTZL010000342         1884 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.345, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000342 DAJTZL010000000
VERSION     DAJTZL010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1884
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.345"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            76..345
                     /locus_tag="OO544_004292"
                     /pseudo
     CDS             76..345
                     /locus_tag="OO544_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937495.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            460..630
                     /locus_tag="OO544_004293"
     CDS             460..630
                     /locus_tag="OO544_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001710241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6769720.1"
                     /translation="MVRLPPVVMSAKSATARTTIAREDAEDHQVKLSAQKLEELLASM
                     VKDEVDRNDGIY"
     gene            complement(757..1314)
                     /locus_tag="OO544_004294"
     CDS             complement(757..1314)
                     /locus_tag="OO544_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCR6769721.1"
                     /translation="MNTAITFSNESPKVFFKDGRLVTTSQAVADYFDKQHKHVLAKID
                     TLDCSPEFTSANFSADVQTVEIGNGAERESRCYLITKDGFMFLVMGFTGKKAARLKEA
                     YIEKFNAMEEELHKRPPAAQNSPAPNNGCALLIHFDKHGQVEFTEKVPADAMVCTLER
                     FKFYLEQHGWIVARKEQLVERLMRF"
     gene            complement(1311..1586)
                     /locus_tag="OO544_004295"
     CDS             complement(1311..1586)
                     /locus_tag="OO544_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HCR6769722.1"
                     /translation="MTVFVLRGYIPHTTAKSVVGIGVSDNITTHSRVCGFFCVKHCHI
                     RIMVGRAGAEKSAPGFVMTGYANPVRLTTSLIGVRGGDHPINWSHHI"
     gene            1771..1884
                     /locus_tag="OO544_004296"
                     /pseudo
     CDS             1771..1884
                     /locus_tag="OO544_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="receptor for colicin S4; incomplete; partial in the
                     middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
BASE COUNT          528 a          387 c          457 g          512 t
ORIGIN      
        1 ttttcattga tttcaccatg ttatagtttt atttgtgaat taaatcaatt atggcgatga
       61 attacaaggg gttaaatgct gccgcagcat agcgatattg aaatagccta gtatgcttcg
      121 atacagcagg agccgaatgg ctggaagacc gtcaccacac agttctacat ccaggaattt
      181 agtgagtata ttgcgccact gcaggatgct gtagatctgg aaatcgcaac ggaggaagaa
      241 agatcgttgc tggaggcatg gaataaatat cgggtattgt tgaatcgtgt tgatacatca
      301 actgcacctg atattgagtg gcctgcaaat cctgtcaggg agtaatcatt gggattatgc
      361 cgcagacacg tcgtatgcag gaacgtgctg cggttagttt gtgagctttc gatagtggtt
      421 gttatttttg cccttatttg ttccggaggc catggttcaa tggtccgtct gccccctgtg
      481 gtgatgtcag caaaatcagc cactgcgcga accacaatag cccgggaaga tgctgaagat
      541 caccaggtaa agctgtcagc gcagaaactg gaagaactgc tcgcatcaat ggttaaggat
      601 gaggttgatc gcaatgatgg gatttattga cgtcagcgag agcagaagga agaactgaat
      661 aacctgaatg atttacgctc aatcagagcg atggcgatta gcggcaatca cgctcgataa
      721 aattaacctc ggtgatcatc cggggttttg cattcattaa aaccgcatca acctttccac
      781 caattgttct ttacgggcaa cgatccaccc atgttgctcc agataaaatt taaaccgttc
      841 cagagtgcat accatcgcat cggcggggac tttttctgtg aactcgacct gaccgtgttt
      901 atcgaagtgg atcagtaatg cgcatccatt atttggggcg ggggagtttt gtgctgcagg
      961 aggacgtttg tggagttctt cttccattgc gttaaatttt tcaatgtaag cttcttttag
     1021 tctggctgct tttttgccag taaatcccat tacgaggaac atgaagccgt cttttgtgat
     1081 caggtagcat cgtgattcac gttctgcgcc gttgccaatt tcgacagtct gaacatcggc
     1141 cgaaaagttg gctgatgtaa attcgggaga gcagtcgagt gtgtcaatct tagctaaaac
     1201 gtgtttatgt tgtttatcga agtagtcggc aacagcttga gaggtggtaa ccaggcgacc
     1261 atctttgaaa aagacttttg gtgactcgtt agaaaaagtt atagctgtat tcatatgtga
     1321 tgactccaat taattggatg atcaccacct ctgacgccaa tcagggaggt ggtgagacgt
     1381 acagggttgg cgtaaccggt catcacaaaa cccggcgcgg atttctccgc ccctgcacgc
     1441 cccaccataa tgcgaatgtg gcaatgctta acgcaaaaaa aaccgcaaac gcggctatgc
     1501 gttgtgatgt tatccgagac gccaatcccg acaaccgatt ttgcggttgt gtgaggaata
     1561 tagccccgga gaacaaaaac tgtcaacggg caaaaaaatt tccgttatca ggaatgtata
     1621 aaattaaagc gatcgagcaa aaaatccttt ctgcaaaact aaaaaagatt tcaataaaat
     1681 caggcagtta aaaacacgga aaacggtata aaaaagcgcc tcaaaatcct gttttttctt
     1741 tcagatacaa tgtcttattg cattttttaa aacatgtcag tgtggtacat ggatatcgat
     1801 accatcgcca attataaact gggcggtgca cagcaacatg atagcgtacg cctcgatccg
     1861 tgggtgttta tgttctcagc agga
//
LOCUS       DAJTZL010000343         1879 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.346, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000343 DAJTZL010000000
VERSION     DAJTZL010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1879
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.346"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(22..384)
                     /locus_tag="OO544_004297"
     CDS             complement(22..384)
                     /locus_tag="OO544_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HCR6769723.1"
                     /translation="MQTKQRLDVPLSLKSVSDSGEFEGYGSVFGVKDSHDDVVMSGAF
                     AASLRAWSDRKALPALLWQHRMDEPIGVYTEMKEDDVGLYVRGRLLIDDDPLAKRAHA
                     HMKAGSLTGLSIGYVLKD"
     gene            complement(488..1834)
                     /locus_tag="OO544_004298"
     CDS             complement(488..1834)
                     /locus_tag="OO544_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element IS4 family transposase"
                     /protein_id="HCR6769724.1"
                     /translation="MFPDFFMHIGQALDLVSRYDSLRNPLTSLGDYLDPELISRCLAE
                     SGTVTLRKRRLPLEMMVWCIVGMALERKEPLHQIVNRLDIMLPGNRPFVAPSAVIQAR
                     QRLGSEAVRRVFTKTAQLWHNATPHPHWCGLTLLAIDGVFWRTPDTPENDAAFPRQTH
                     AGNPALYPQVKMVCQMELTSHLLTAAAFGTMKNSENELAEQLIEQTGDNTLTLMDKGY
                     YSLGLLNAWSLAGEHRHWMIPLRKGAQYEELRKLGKGDHLVKLKTSPQARKKWPGLGN
                     EVTARLLTVTRKGKVCHLLTSMTDAMRFPGGEMADLYSHRWEIELGYREIKQTMQLSR
                     LTLRSKKPELVEQELWGVLLAYNLVRYQMIKMAEHLKGYWPNQLSFSESCGMVMRMLM
                     TLQGASPGRIPELMRDLASMGQLVKLPTRRERAFPRVVKERPWKYPTAPKKSQSVA"
BASE COUNT          398 a          534 c          473 g          474 t
ORIGIN      
        1 gtccgttaaa ctcaggctac ctcagtcttt caggacgtac ccaatagaaa ggccggttaa
       61 cgaaccggcc ttcatgtgtg catgtgcacg ttttgcgagg ggatcatcat caataagcaa
      121 ccgtcccctg acgtaaagcc cgacatcgtc ttccttcatt tcggtgtaaa caccgatggg
      181 ttcatccatg cggtgctgcc agagcagcgc aggtaacgct tttctgtcac tccacgcccg
      241 cagggaagca gcaaatgccc cggacatcac cacatcatcg tggctgtcct ttacaccaaa
      301 gacggagcca tacccttcaa actcaccgga gtcactgaca gatttcagac tcagcggtac
      361 atcaagacgt tgtttcgtct gcattggcgt tatccttctg cttaccggcc ttactgccat
      421 cggagggttt cgtggtcatg ttcatcggtg tgagatagac atcaccaccg ggactaatgc
      481 cagtcagtta agcaactgac tggctctttt tcggggctgt ggggtatttc cagggcctct
      541 cctttaccac tctcgggaag gccctttccc ttctcgtcgg taatttcaca agttgtccca
      601 tacttgcaag atcgcgcatc agctccggta tacgtcccgg tgaagcgccc tgcaatgtca
      661 tcagcattct catcaccatt ccgcatgatt ctgagaaact cagttgattc ggccagtaac
      721 ctttcagatg ttccgccatt ttaatcatct gatatctcac cagattataa gccagtaaga
      781 caccccacag ctcttgctcc acaagctccg gctttttact tctcagcgtc agcctgctca
      841 gttgcatcgt ctgttttatc tccctgtatc ccagttcgat ttcccagcga tgactgtaca
      901 gatccgccat ttctcctccg gggaagcgca tggcgtccgt catcgacgtc agcagatggc
      961 agacttttcc tttgcgcgtc acggtcagca ggcgggctgt cacttcattt cccagtcccg
     1021 gccacttttt tcgtgcctgc gggctggttt tcagcttcac cagatgatcg cctttaccca
     1081 gttttctgag ctcttcatat tgcgctccct ttctgagagg tatcatccag tggcggtgtt
     1141 ctcccgccag gctccaggca tttaacagtc ccagtgagta ataaccttta tccattaacg
     1201 tcagagtgtt atcgccggtt tgttctataa gttgctcagc aagctcattt tcgctgttct
     1261 tcatcgtgcc gaaggctgca gccgtcagca gatggctggt cagttccatc tggcagacca
     1321 ttttgacctg cgggtagagc gccgggttcc cggcatgtgt ctggcggggg aaggctgcat
     1381 cgttctctgg tgtatccggt gtgcgccaga acacaccatc gatggccagc agggtcaggc
     1441 cgcaccagtg cggatgcggc gtggcgttat gccagagctg cgctgttttc gtgaacacgc
     1501 ggcggacagc ctcacttccc aggcgctggc gggcctgaat aacggcactg ggggcaacga
     1561 aggggcgatt gcccggcagc atgatgtcca ggcgattcac aatctggtga agaggttctt
     1621 tacgctcaag cgccatgcca acaatacacc agaccatcat ttcgagggga agacggcgct
     1681 tgcgtagcgt tacagtacct gattcggcaa ggcaacgaga gatgagttcg gggtcgaggt
     1741 aatcccccag agaagtcagt gggttacgca gagaatcgta acgggatacc agatcaagag
     1801 cctgtccaat gtgcataaaa aaatccggaa acaagtgagc gtttccggat tcttacacag
     1861 ccactggatc ggtcaactg
//
LOCUS       DAJTZL010000344         1818 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.347, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000344 DAJTZL010000000
VERSION     DAJTZL010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1818
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.347"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(8..859)
                     /gene="hchA"
                     /locus_tag="OO544_004299"
     CDS             complement(8..859)
                     /gene="hchA"
                     /locus_tag="OO544_004299"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase HchA"
                     /protein_id="HCR6769725.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHGDNPLNGYSICAFPDAADKQTP
                     EIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKVLTGDSPFAANALGKLAAQE
                     MLAAYAD"
     gene            1115..1310
                     /locus_tag="OO544_004300"
                     /pseudo
     CDS             1115..1310
                     /locus_tag="OO544_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353128.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1450..>1818)
                     /locus_tag="OO544_004301"
     CDS             complement(1450..>1818)
                     /locus_tag="OO544_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HCR6769726.1"
                     /translation="AMMYAETRNMTPYGNVGIANKTQNFEAVAQYQFDFGLRPSLAYV
                     YSKGKDLGGNDYNNNGHQEYVDQDLVNYVEIGATYYFNKNFSTYVDYKINLLDKDDDF
                     YDNNGIATDDVVGVGLVYQF"
BASE COUNT          478 a          370 c          445 g          525 t
ORIGIN      
        1 acagggatta atccgcataa gctgccagca tttcctgcgc cgccagttta cccaacgcat
       61 tcgctgcgaa aggactgtcg ccggtgagaa ctttacggtc cttatgtact cgcccggtga
      121 tgtcgtcatt aataatgttc atgcccattt tcttcagctc ttcgccgaag taccaggtga
      181 gatgacccgg catatagcct atctctggcg tttgtttgtc tgcggcgtct gggaatgcgc
      241 aaatggaata accattcagt gggttatcgc cgtggcgaag cgccagaaaa gccgccgggc
      301 cgtggcaaag ggagataaca aaacggtcat ttttgattgc ccactgcaaa gcagcagcta
      361 cgtcctggct ttctggtaaa ccaataagtg cgccatgacc accaggaaca aagattgctg
      421 catattcgct atcagcgttg aggctggcga caacatccgc gagtttcttc ggattgcgga
      481 acaacgattt atgctgctca aagaatggca ttactttttc atctttgtgc ggcatagccc
      541 agtattcaaa cttggtcatc agaccggaaa tggtcgccac ttcgaattcg aaaccagcag
      601 catggagatg atacaacggc agcaacgttt caatcggatg gttaccggtc gagaacagtt
      661 ttccgttatc ggttggcaaa taacgttcgt ccgcggcgat caccagaatt ttatgcttac
      721 cgcgatacgg ttttggatag tccacgccat caagatcaga gacgggactg gtatattggc
      781 taagcgaata ttctgaaggg aagaatgcat tatcttcagc aatatcgacc tgcggatttt
      841 tacttgtttg aacagtcata gtgtattcct tattgttgct tagttagggt agtcactata
      901 tttgcgactg agctggtcgc tatttttaac cagtctggaa aattgagtca atatatcgaa
      961 agcggtagtc ggcgggtgta agtatgtaat gatgatgctg cactggtgtt gaatattatt
     1021 cacgccaaat tttccttatt gagcaacgtc gtcgcaagtg gcctcaatga ttttaggcca
     1081 ttatcaaagg tacggggcgg cggggagaga tttagtgctg tgaaatattg tggtactgat
     1141 gaatatttat atcgcggatt ggtagagaaa agattatttc atgcactatt tttgcaaata
     1201 tttctatatg ggaagcgtgt ttgttacgga ttaattcata aagaaatgca tttgggtgtt
     1261 ggtttgaaga gtcgctggtt gatgttattg tgccaggagg ggcagggtaa ggtgctgtat
     1321 cgatatgcat tggcaattgc gtgtgccgca gatggagatg aaatgccaat aaaaaagcct
     1381 gaattcgatg agggacgaac tcaggctagt gtttagtttt acagtaacac gccgcagcac
     1441 gttggctgat tagaactggt agactaagcc cacaccaaca acgtcatctg ttgcgatgcc
     1501 attgttgtca tagaaatcgt catctttatc caacaagttt attttatagt caacatatgt
     1561 tgagaagttc ttattgaaat agtaggtcgc tccaatttca acatagttga ccagatcctg
     1621 atcaacatac tcctgatgac cattgttgtt atagtcgtta ccgccgaggt ctttgccttt
     1681 tgaataaaca tatgcaagag atgggcgcag accaaaatcg aactgatact gtgctacagc
     1741 ttcaaagttc tgggttttat ttgcgatacc cacattacca tatggtgtca tattgcgggt
     1801 ttcagcatac atcattgc
//
LOCUS       DAJTZL010000345         1812 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.348, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000345 DAJTZL010000000
VERSION     DAJTZL010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1812
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.348"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..486
                     /locus_tag="OO544_004302"
     CDS             <1..486
                     /locus_tag="OO544_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6769727.1"
                     /translation="THSASASSTDLGTKTTSSFDYGTKSTNNTGAHTHSLSGSTSSAG
                     AHQHSQTGPRTNSGSQPTGMFPAGSTQVSGTNQVGISGSLTSGTSQWVGKSSSEGNHT
                     HSLSGTAASAGAHAHTVGIGAHTHSVAIGSHGHTITVNAAGNAENTVKNIAFNYIVRL
                     A"
     gene            486..1067
                     /locus_tag="OO544_004303"
     CDS             486..1067
                     /locus_tag="OO544_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR6769728.1"
                     /translation="MAFRMSEQARTIKIYNLLAGTNEFIGEGDAYIPPHTGLPANSTD
                     IAPPDIPAGFVAVFNSDESSWHLVEDHRGKTVYDVASGDALFISELGSLPENVTWLSP
                     EGEFQKWNGTAWVKDTEAEKLFRIREAEETKNSLMQVASEHIAPLQDAVDLEIATEKE
                     TLLLEAWKKYRVLLNRVDTSTAPDIEWPVAPIG"
BASE COUNT          572 a          350 c          417 g          473 t
ORIGIN      
        1 acccacagcg ccagcgcatc cagtacggat ttggggacga aaaccacatc gtcgtttgat
       61 tacggcacta aatccacgaa taacaccggg gcgcatacgc acagtctgag cggctctaca
      121 agttcagctg gggcccacca acatagtcag actgggccca gaacgaatag cgggagtcaa
      181 cctactggaa tgtttccggc agggagtact caagttagcg ggactaatca ggttggtatc
      241 agtggctcat taaccagtgg cacctcccaa tgggtaggta aatcatcttc agaaggaaac
      301 catacacact cactgtccgg cacagcagca tctgcgggtg cacacgcgca tactgtcggt
      361 attggtgctc atacgcactc tgttgcgatt ggttcacatg gacacaccat caccgttaac
      421 gctgctggta acgcggaaaa caccgtcaaa aacatcgcat ttaactatat tgtgaggctc
      481 gcataatggc attcagaatg agtgaacaag cacggaccat aaaaatttat aacctgctgg
      541 ccggaactaa tgaatttatt ggtgaaggtg acgcatatat tccgcctcat acaggtctgc
      601 ctgcaaacag taccgatatt gcaccgccag atattccggc tggtttcgtg gctgttttca
      661 acagtgatga gtcatcgtgg catctcgttg aagatcatcg gggtaaaacg gtttatgacg
      721 tggcttccgg cgacgcgtta tttatttctg aactcggctc attaccggaa aatgtcacct
      781 ggttatcccc ggaaggggag tttcagaagt ggaacggcac agcctgggtg aaggatacgg
      841 aagcagaaaa actgttccgg atccgggagg cggaagaaac aaaaaacagc ctgatgcagg
      901 tagccagtga gcatatagcg ccgcttcagg atgctgtgga tctggagatc gcaacggaga
      961 aagaaacctt gttgctggaa gcttggaaaa agtatcgggt gttgctgaac cgtgttgata
     1021 catcaactgc accggatatt gaatggccag tagcacctat agggtaaatt cgtaatgatt
     1081 acctaaatac gttattcttt tgttaaaaaa gtgattttgc tataggtagg gagtatgttc
     1141 ttggattaag aataaaaaat cgccatgaaa gagcttcatg gcgagtacat aaacgtacaa
     1201 aatgtgatga aagtagagtg gcgtaagcca ctatattaag atatgacaat agcgaagaaa
     1261 agtaaataac attttatgga cgtttaattg ttaattataa aaactagagt gcccgatatt
     1321 gtttatcttg ctttaaaata atgtactcaa accaaatttt tgagctaaaa gctcaaataa
     1381 tataaatgca ataataatgt ttaataagac aattagcttg ctttgtattt tcatttcgga
     1441 atactgttaa gtaactttga tgaccctaac aatagatcgg aattttctta gagtcaatgg
     1501 tcactgtttt tgcttgcaaa gcaaatgttt tggtggttat aagtcgtctt aatatagtca
     1561 cgcatgcaac ttgttccatg cggagattaa agattggaat ataacgcgaa gatggaatta
     1621 aattacattt gcaataatta atttattatt actcaataat taccgatatt attattgtaa
     1681 attaatcatt agaatgcaaa aagaaatgta acggcaaaat aagaggacga tagctgagaa
     1741 aaaagcgtgc tttcccaatc tggggcaaat cacgtctgaa tgacacctga aaacaggtgg
     1801 tagcctcaag aa
//
LOCUS       DAJTZL010000346         1790 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.349, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000346 DAJTZL010000000
VERSION     DAJTZL010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1790
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.349"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..1463)
                     /locus_tag="OO544_004304"
     CDS             complement(<1..1463)
                     /locus_tag="OO544_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HCR6769729.1"
                     /translation="MDKKLLALLILASLSPAEATLTEIPAGFEVIAQGQQEYIEVYFS
                     GKSLGKYYAMVNLDTVTFLDPASLYNKLELDVDDQKIAHIVKEKLSQPLAHHGELACG
                     YVRTDSGCGFLNTDTLEIIYNDEESSATLFINPQWNSAFDAKSLYLNPDKNTVNAFIH
                     QQDINVLAQDDYQSLSIQGNGALGITENSYIGAHWNFNGYDADDVSDSNADVSDLYYR
                     YDFLRRYYVQAGRMDNRTLFNAQGGNFTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGT
                     PVMVLLSRNSRVDAYRNEQLLGSFYLNSGSQFIDTSSFPPGSYSVALKVYENNQLTRT
                     ELVPFTKTGGLTDGNAQWFLQAGKTTSQVSDDESSAYQLGVRLPLHPQYELYAGLANA
                     DDVSAFELGNNWTADLGGVGNLAISASVFRNDDGGKGDMQQANWSNPGWPTLGFYRTN
                     SDGDACTTDSRESYNALSCYESISATVSQNFVGWNMML"
     gene            complement(1555..1767)
                     /locus_tag="OO544_004305"
                     /pseudo
     CDS             complement(1555..1767)
                     /locus_tag="OO544_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755097.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
BASE COUNT          492 a          434 c          367 g          497 t
ORIGIN      
        1 agcatcatat tccagccgac aaaattctgt gaaaccgtcg cggaaatact ttcataacag
       61 cttaaggcgt tatagctctc tctgctgtcg gttgtacaag catcgccgtc agagttggtc
      121 cgataaaagc ccaacgtcgg ccatcccgga ttactccagt tggcctgttg catatcacct
      181 ttgccgccgt catcgttacg gaacacgctg gcgctgattg caagattccc caccccgcct
      241 aaatctgccg tccagttatt acctaactcg aaagcactca catcatcggc attcgccagc
      301 cctgcgtaga gctcatattg cggatgtaat ggcaggcgta ctcctagctg ataagctgaa
      361 ctttcatcat cagaaacctg tgatgtagtt ttacctgcct gtaagaacca ttgcgcattt
      421 ccgtcagtca gaccgccggt tttggtaaac ggcacaagct cggtgcgggt gagttggtta
      481 ttttcgtaga cttttaatgc cacgctgtag ctgcctggcg gaaaggaact ggtatcaata
      541 aattgcgaac cactattgag ataaaacgat cccaaaagtt gctcattacg ataagcgtca
      601 acacgagaat tgcgcgaaag cagaaccatt actggggttc cttgctggct ttgcgcctgg
      661 tttaaatagc tcagtgtcga cccgatacgc atcccatcaa ttgcaccgag tggcagaaag
      721 ttaaaggtaa agttcccgcc ttgtgcatta aatagtgtgc ggttgtccat gcgacccgcc
      781 tgcacataat aacgacgtaa aaaatcataa cgataataga gatcgctgac gtcagcatta
      841 ctgtcactga catcatctgc atcataaccg ttgaaattcc agtgtgcacc aatataacta
      901 ttttctgtta ttcccagcgc accgtttccc tgaatagaca acgattggta atcatcctgt
      961 gccagaacat tgatgtcttg ctgatgtata aaagcattaa cagtattttt gtctggattt
     1021 aaatataatg acttcgcatc gaaagctgaa ttccattgcg gattaataaa caacgttgcc
     1081 gaactttctt catcattata gattatttcc agcgtatcgg tattcagaaa accacaccct
     1141 gagtcagtac gtacataacc gcaagccaat tcaccgtggt gagctagcgg ctgcgataat
     1201 ttttctttca ctatatgcgc gattttctga tcgtctacat ccagttccag cttgttatat
     1261 agacttgctg gatcaagaaa tgttacggta tcaagattaa ccattgcata atatttaccg
     1321 agacttttcc ctgaaaaata aacctcgata tactcctgct gtccctgagc aataacctca
     1381 aaccctgcgg gaatttcggt taatgtcgcc tctgccgggc tgagactcgc caggatcaaa
     1441 agagccagta attttttatc catggcaatc ctgaaatcca taacaaatac taaaacagtt
     1501 aaaataaggg agggctaccc ctccctttgt tacgtgttaa ttctgttatc tggattagat
     1561 atcctgagac agataaatgc ttactacacc acggtagatg cctgtctcca gaacaccttt
     1621 ggtggtttga gcaaacatca ggtttttagc cagagaaccg ttagttaacg catcggaagc
     1681 aaagattttg gttgcggtaa aagtggttgc cgcatcagct ttaatttctt caccgcccca
     1741 ggttactgtc agcggaacaa tcttgctaaa tagctgcgct taataccgct
//
LOCUS       DAJTZL010000347         1779 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.350, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000347 DAJTZL010000000
VERSION     DAJTZL010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1779
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.350"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..1528
                     /gene="tssI"
                     /locus_tag="OO544_004306"
     CDS             <1..1528
                     /gene="tssI"
                     /locus_tag="OO544_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HCR6769730.1"
                     /translation="VSTFCISQFRYSAQIRPSSVVTKDYTFKRPGWAGRFDQEGQYQD
                     YQRTQYEVYDYPGRFKGAHGQNFARWQMDGWRNNAEVARGTSRSPEIWPGRRIVLTGH
                     PQANLNREWQVVASELHGEQPQAVPGRSGSGTTLNNHFAVIPADRTWRPQPLLKPLVD
                     GPQSAVVTGPAGEEIFCDEHGRVRVKFNWDRYNPSNQDSSCWIRVAQAWAGTGFGNLA
                     IPRVGQEVIVDFLNGDPDQPIIMGRTYHQENRTPGSLPGTKTQMTIRSKTYKGSGFNE
                     LKFDDATGKEQVYIHVQKNMNTEVLNNRTTDVINNHAEKIGNNQAITVTNNQIQNIGV
                     NQIQTVGVNLVETVGSNQIIKVGSNQVEKVGIIRALTVGVAYQTTVGGIMNTSVALLQ
                     SSQVGLHKSLMVGMGYSVNVGNNVTFSVGKTMKENTGQTAVYSAGEHLELCCGKARLV
                     LTKDGSIFLNGTHIHLEGESDVNGDAPVINWNCGATQPVPDAPVPKDLPPGMPDMRQF
                     "
     gene            1604..>1779
                     /locus_tag="OO544_004307"
     CDS             1604..>1779
                     /locus_tag="OO544_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
                     /protein_id="HCR6769731.1"
                     /translation="MGGGKPAARQGDMTRKGLDIVQGSAGVLIGAPTGVACSVCPGGI
                     TYANPVNPVLGAKVL"
BASE COUNT          455 a          427 c          562 g          335 t
ORIGIN      
        1 ggtaagtacc ttctgcatca gccagttccg ctacagcgca caaatccgtc cttcttccgt
       61 ggtgaccaaa gactacacct ttaaacgacc cggctgggca gggcgttttg atcaggaagg
      121 ccagtaccag gattaccagc gcacacagta tgaagtgtat gactaccccg gacgtttcaa
      181 gggtgctcac gggcagaact ttgcccgctg gcagatggat ggctggcgca acaacgcaga
      241 agtggcgcgc ggaacaagcc gttctccgga gatatggccg ggacggcgaa ttgtgctgac
      301 ggggcatccg caggcgaacc tgaaccggga atggcaggtg gtggcaagtg aactgcacgg
      361 cgagcagccg caggcggtgc cgggacgcag tggttcagga accacgctga ataaccactt
      421 tgcggtaata ccggcagacc ggacatggcg gccacagccg ttgctgaaac cgctggtgga
      481 tggcccgcag agcgccgtcg tgacgggacc ggcaggcgag gaaatcttct gtgatgaaca
      541 tggccgcgtg cgggtgaaat ttaactggga ccgctataac ccgtcaaacc aggacagttc
      601 atgctggatc cgtgtggcac aggcgtgggc aggcaccgga ttcggtaacc ttgcgatacc
      661 gcgcgtgggt caggaggtca ttgtggactt cctcaacggc gatccggacc agccgattat
      721 tatggggcgt acctaccacc aggaaaaccg cacacccggt agcctgccgg ggacgaagac
      781 gcagatgacc attcgttcga aaacctataa gggcagcggg tttaatgaac tgaagtttga
      841 cgatgcgaca gggaaagaac aggtctacat ccacgtgcag aagaacatga acaccgaggt
      901 gctgaataac cgcaccacgg atgtgataaa caaccatgcc gaaaaaatag gtaacaacca
      961 ggcgatcacc gttaccaata accagatcca gaacattggc gttaatcaga tacagacggt
     1021 tggtgtcaac ctggtggaaa cggtgggcag taaccagatt atcaaagtgg gatcaaacca
     1081 ggttgaaaag gtggggatca ttcgtgcgct gacggtgggt gtggcttacc agacgacggt
     1141 aggcggcatt atgaatacct cggtggcgtt gttgcagtcc tcacaggtag ggctgcataa
     1201 atcactgatg gtgggaatgg gctacagcgt caatgtaggg aataacgtca ccttctcggt
     1261 gggcaagacg atgaaggaaa acaccggaca aacagcagtt tattctgccg gtgagcatct
     1321 tgaactctgc tgtggtaagg caaggctggt gctgacgaag gacggaagca tatttctcaa
     1381 cggtacgcac attcatctgg aaggggagtc ggatgtgaac ggtgatgcgc cagtgattaa
     1441 ctggaactgt ggtgccacac aacctgtacc ggatgcgcct gtgccgaaag atttaccccc
     1501 cggaatgccg gatatgcggc aattttgagt caggtgcaaa aaattaaaac ggaactggtg
     1561 cagtgaccag atgaaccata ttaaaagcag aaggaattga ttcatgggcg gcggaaaacc
     1621 ggcggcgcgg cagggcgaca tgacccgcaa gggactggat attgtgcagg gttcagcggg
     1681 tgtgctgata ggtgcgccga cgggagtggc ctgttcggtg tgtccgggag ggattaccta
     1741 tgctaacccg gtgaacccgg tgctgggtgc gaaggtgct
//
LOCUS       DAJTZL010000348         1754 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.351, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000348 DAJTZL010000000
VERSION     DAJTZL010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1754
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.351"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            126..1502
                     /gene="hpaX"
                     /locus_tag="OO544_004308"
     CDS             126..1502
                     /gene="hpaX"
                     /locus_tag="OO544_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HCR6769732.1"
                     /translation="MSDTSPAIPESIDPANQHKALTAGQQAVIKKLFRRLSVFLFVLF
                     IFSFLDRINIGFAGLTMGRDLGLSATMFGLATTLFYAAYVIFGIPSNIMLSIVGARRW
                     IATIMVLWGIASTATMFATGPTSLYVLRILVGITEAGFLPGILLYLTFWFPAYFRARA
                     NALFMVAMPVTTALGSIVSGYILSLDGVMALKGWQWLFLLEGFPSVLLGVMVWFWLDD
                     SPDKAKWLTKEDKKCLQEMMDNDRLTLVQPEGAISHHAMQQRSMWREIFTPVVMMYTL
                     AYFCLTNTLSAISIWTPQILQSFNQGSSNITIGMLAAVPQICTILGMVYWSRHSDRRQ
                     ERRHHTALPYLFAAAGWLLASATDHNMIQMLGIIMASTGSFSAMAIFWTTPDQSISLR
                     ARAIGIAVINATGNIGSALSPFMIGWLKDLTGSFNSGLWFVAALLVIGAGIIWAIPMQ
                     SSRPRATL"
     gene            1512..>1754
                     /locus_tag="OO544_004309"
     CDS             1512..>1754
                     /locus_tag="OO544_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003924737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HCR6769733.1"
                     /translation="MCDRQIANIDISKEYDESLGTDDVHYQSFARMAAFFGRHMLPHR
                     HEQYFQMHFLNSGQIELQLDDHRYSVEAPLFVLTPPS"
BASE COUNT          375 a          480 c          463 g          436 t
ORIGIN      
        1 ccggataagg cgtttacgcc gcatccggca aaaagccgta ccaaatcgcg gaccggataa
       61 ggcgttacgc cgcatccgcc ataaaccttg tcgtacccta caaaaatccc attagaggaa
      121 gaaaaatgag cgacacctca cctgccatac cggagagtat cgatccggcg aatcagcata
      181 aagcgctgac tgccggacaa caggcggtta ttaagaagct atttcgccgc ctgagcgtct
      241 ttctgttcgt gctgtttatc ttctcgttcc ttgatcgcat caacatcggc tttgccggac
      301 tcacgatggg acgcgacctc ggtctgagcg ccaccatgtt tggcctcgct accaccctgt
      361 tctatgccgc ttatgtcatc ttcggcattc ccagcaacat tatgctgagt attgtcggtg
      421 cgcggcgctg gatcgccacc atcatggtgc tctggggcat cgcctctact gccaccatgt
      481 ttgccactgg ccccaccagc ttgtacgtac tgcgtatact ggttggcatt accgaagccg
      541 gctttctgcc tggtattctg ctgtatttaa ccttctggtt tccggcctat ttccgcgccc
      601 gtgccaacgc cttgtttatg gtagcaatgc cggtaacgac agcgttgggc tcgatcgttt
      661 ccggctacat tttgtcgctg gatggcgtca tggcattaaa aggctggcag tggctgtttt
      721 tgctggaagg cttcccgtcg gtattactcg gcgtcatggt gtggttctgg cttgatgact
      781 caccggacaa agctaagtgg ctgacgaaag aagacaaaaa atgcctgcaa gagatgatgg
      841 ataacgatcg tctgacgctg gttcagccag agggagccat cagccaccac gccatgcaac
      901 aacgcagtat gtggcgggag atcttcactc cggtggtgat gatgtatacc ctggcgtatt
      961 tctgcctgac caacacactt agtgcgatca gcatctggac accgcagatc ctgcaaagtt
     1021 ttaatcaggg cagcagtaat atcaccatcg gcatgctggc cgccgtaccg cagatttgta
     1081 ccattctcgg gatggtctac tggagccgtc actcagatcg ccgccaggaa cgaaggcatc
     1141 acaccgccct tccttatttg ttcgctgccg caggttggtt actggcttcg gcaactgatc
     1201 acaacatgat ccagatgctg gggatcatta tggcttcgac cggatcattc agcgcaatgg
     1261 cgattttctg gacaacaccg gatcagtcca tcagcctgcg ggcacgagcg atcggtattg
     1321 cggtgatcaa cgccactggc aacattggtt cagcgttaag tccgtttatg atcggctggt
     1381 tgaaagattt gaccggcagc tttaacagtg gattgtggtt tgttgccgcg ctgctggtga
     1441 ttggtgcggg gattatctgg gcgattccaa tgcagtcctc ccgtccgcga gcgaccctgt
     1501 aaggaacgac gatgtgtgac cgtcagattg ccaatattga tatcagcaaa gagtacgatg
     1561 aaagcctggg cacggacgat gtgcattatc agtcgttcgc ccgcatggcg gcattttttg
     1621 gccgccatat gctgccacat cgccacgaac agtactttca gatgcatttc ctcaatagcg
     1681 gacagattga gctacagctt gacgatcatc gctactcggt ggaagcgccc ctgtttgtcc
     1741 tgacgccgcc gtca
//
LOCUS       DAJTZL010000349         1717 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.352, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000349 DAJTZL010000000
VERSION     DAJTZL010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.352"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..1484
                     /gene="tssI"
                     /locus_tag="OO544_004310"
     CDS             <1..1484
                     /gene="tssI"
                     /locus_tag="OO544_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HCR6769734.1"
                     /translation="CISQFRYSAQIRPSSVVTKDYTFKRPGWPGRFDQEGQYQDYQRT
                     QYEVYDYPGRFKGAHGQNFARWQMDGWRNNAEVARGTSRSPEIWPGRRIVLTGHPQAN
                     LNREWQVVASDLHGEQPQAVPGRRGSGTTLENHFAVIPADRTWRPQPLMKPLVDGPQS
                     AVVTGPAGEEIFCDEHGRVRVKFNWDRYNPSNQDSSCWIRVAQAWAGTGFGNLAIPRV
                     GQEVIVDFLNGDPDQPIIMGRTYHQENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFD
                     DATGKEQVYIHAQKNMNTEVLNNRTTDVINNHAETIGNNQMIAVTNNQIETVGVNQIE
                     TVGSNQIIKVGSVQVETIGLVRALTVGVAYQTTVGGIMNTSVALMQSSQIGLHKSLRV
                     GLGYDVKVGNNVTFTVGKTKKDDTGQTAIYSAGEHLELCCGKARLVLTKDGQIFLNGT
                     KIHLQGKEQVNGDSLLINWNCAASKSPPKTPDEKQDTPDMREY"
     gene            1552..>1717
                     /locus_tag="OO544_004311"
     CDS             1552..>1717
                     /locus_tag="OO544_004311"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6769735.1"
                     /translation="MSGKPAARQGDMTQYGGPIVQGSAGVRIGAPTGVACSVCPGGMT
                     SGNPVNPLLGA"
BASE COUNT          440 a          419 c          533 g          325 t
ORIGIN      
        1 tctgcatcag ccagtttcgc tacagcgcac aaatccgtcc ttcttccgta gtgaccaaag
       61 actacacctt taaacgtccc ggctggccag ggcgttttga tcaggaaggc cagtaccagg
      121 attaccagcg cacgcagtat gaagtgtatg actaccccgg acgtttcaag ggtgcccacg
      181 ggcagaactt tgcccgctgg cagatggacg gctggcgaaa caacgcagaa gtggcgcgcg
      241 gaacaagccg ttcgccggag atatggccgg gacggcgaat tgtgctgacg gggcatccgc
      301 aggcgaacct gaaccgggaa tggcaggtgg tggcgagcga tcttcacggc gaacagccgc
      361 aggcggtacc gggacgcagg ggttcaggca ccacgctgga gaaccatttt gcggtaatcc
      421 cggcagacag aacatggcgg ccacagccgt tgatgaaacc gctggtggat ggcccgcaga
      481 gcgccgtcgt gacgggaccg gcaggcgagg aaatcttctg tgatgaacat ggccgcgtgc
      541 gggtgaaatt taactgggac cgctataacc cgtcaaacca ggacagttca tgctggatcc
      601 gtgtggcaca ggcgtgggca ggcaccggct ttggcaacct ggcgataccg cgtgtgggtc
      661 aggaggtgat tgtggacttc ctcaacggcg acccggacca gccgattatt atggggcgta
      721 cctaccacca ggaaaaccgc acacccggca gcctgccggg gacgaagacg cagatgacca
      781 ttcgttcgaa aacctataag ggcagcgggt ttaatgaact gaagtttgac gatgcgacag
      841 ggaaagaaca ggtctacatc cacgcgcaga agaacatgaa caccgaggtg ctgaataacc
      901 gcaccacgga tgtgataaac aaccatgctg aaaccattgg caacaatcag atgattgcgg
      961 ttaccaacaa tcagatagag acggtgggcg ttaaccagat agagacggtg ggcagtaacc
     1021 agataattaa agtgggttct gttcaggtgg aaacgattgg acttgttcgt gcgctgacgg
     1081 tgggcgtggc gtatcagacg acggtaggtg gcattatgaa cacctcggtg gcactgatgc
     1141 agtcctcgca gatcggtttg cataaatcgt tgagggtcgg gctgggttat gacgtcaaag
     1201 tcggaaataa cgttaccttc accgttggta aaacgaaaaa ggatgatacc gggcagaccg
     1261 cgatttactc cgccggtgag catctggagc tctgctgtgg taaggcaagg ctggtgctga
     1321 cgaaggacgg acaaattttt ctcaacggca caaaaattca tttgcagggt aaggagcagg
     1381 ttaatggtga ctcactgttg attaactgga actgtgcagc ctcgaaatcc ccaccgaaga
     1441 cccctgatga aaagcaggat acgccggata tgagagagta ctgatcccgt acctgttcgg
     1501 tattcagtaa caaaccagca ttaaaaataa acagaagaag gaatttgaat catgagcgga
     1561 aaaccagcgg cgcgtcaggg agatatgact cagtatggcg gtcccattgt ccagggttcg
     1621 gcaggtgtaa gaattggcgc gcccaccggc gtggcgtgct cggtgtgtcc gggcgggatg
     1681 acttcgggca acccggtaaa tccgctgctg ggggcga
//
LOCUS       DAJTZL010000350         1710 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.353, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000350 DAJTZL010000000
VERSION     DAJTZL010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1710
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.353"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(50..193)
                     /locus_tag="OO544_004312"
                     /pseudo
     CDS             complement(50..193)
                     /locus_tag="OO544_004312"
                     /inference="COORDINATES: protein motif:HMM:NF024729.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(197..1018)
                     /locus_tag="OO544_004313"
     CDS             complement(197..1018)
                     /locus_tag="OO544_004313"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR6769736.1"
                     /translation="MFSHPVYLFLREFSLQDSRGGSLSKKAESLSSGKITIGTKEKVI
                     IINELRQCHPLSQLLVIADLPRSTFYYHVKRLNAPDPYQLVKQVILRIYHQHKGRYGY
                     RRIRLACRNESILLNGKTIRKLMKELGISSLIRRKKYRTYRGEQGRTCNNLLKRQFYA
                     DRPNQKWVTDVTEFKVDGRKLYLSPIMDLYNGEIVSYNLTERPLASMVKSMLLDAVEQ
                     LNKDDKPLLHSDQGWQYQMPRWQRWLSDNGITQSMSRRGNCLDNAAMESFFSTLK"
     gene            1007..1710
                     /locus_tag="OO544_004314"
                     /pseudo
     CDS             1007..1710
                     /locus_tag="OO544_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    1287..1403
                     /locus_tag="OO544_004314"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
BASE COUNT          440 a          380 c          402 g          488 t
ORIGIN      
        1 cgtaggtgcc caccttttca aatgatctgc gccccaaatc ttggacatcc tatgaaaaat
       61 gtttaagtcg atattgaacc ggactcatgc catcgagttt ctctttaatg cgatggtgat
      121 tgtagtattc aatgtaatca attatatctt gctttagatg atgtaactta ttgtatttat
      181 ttaggtagta acattcttat tttaaagtac taaaaaagct ttccattgct gcattatcta
      241 agcagttacc acgtcgggac atgctttgtg ttatgccatt atcactcaac caacgttgcc
      301 aacgaggcat ctgatattgc cacccctgat cactatgcaa caagggctta tcgtctttat
      361 tgagttgttc taccgcatct aatagcatgc tctttaccat tgatgctaat gggcgctcag
      421 taaggttata tgagactatt tctccattat acaggtccat gattggtgat aaatagagct
      481 tcctgccatc gaccttaaac tctgtgacat ctgttaccca cttttgattt gggcggtcag
      541 cataaaattg tctcttcagt aaattattac aagttcgccc ctgttctcca cgataggtac
      601 ggtacttctt gcgcctgata agtgagctga tacccagttc cttcattaac tttctgatgg
      661 ttttaccatt aagtaaaatg ctctcgttac ggcaggcaag tctgatgcgt cgataaccat
      721 aacgcccttt atgctgatgg tatattctca atattacttg tttaacaagt tgatatggat
      781 ctggcgcatt aaggcgtttc acgtgatagt aaaatgtact acgtggcaaa tccgcgataa
      841 ccagtaattg tgataatgga tgacattgcc ttaactcatt aataatgatt actttttctt
      901 ttgttccaat cgtaattttt cctgagctaa ggctttcagc ttttttagat aatgaaccgc
      961 cccgggaatc ctggagacta aactccctga gaaagaggta aacaggatga ctaaaaatac
     1021 tcgttttccc ccgaagtccg tcaacgggca gtccgtatgg ttctggaaag tcagggcgaa
     1081 tatgactcac aatgggcggt aatttgttcc attactccaa agattggctg tacaccggag
     1141 actctgcgtg tctgggttcg ccagcatgag cgggatacca ggggcggtga tggagggctc
     1201 accaccgctg aacgtcagcg tctgaaagag ctggagcgtg agaatcgtga actgcgctgc
     1261 agtaacgata tccttcgcca ggcttccgct tattttgcga aggcggagtt cgaccgcctc
     1321 tggaaaaaat aatgccactg ctggataagc tgcgtgggca gtacggggtc ggaccggtat
     1381 gcagtgaact gcatattgcc ccgtcaacgt attaccactg tcagcaacag gacatcatcc
     1441 tgataaacgc agtgcccgtg cgcagcacga tgactggctg aagagagaga tacagcgcgt
     1501 atacgatgaa aatcatcagg tgtacggtgt gcgtaaagtc tggcgccagt tgttacggga
     1561 aggaatcagg gtggccagat gtacagtggc gcgcctcatg gcggttatgg gacttgtcgg
     1621 tgttctccgg ggtaaaaagg tccgcactac cgtcagccgg aaaaccgttg ccgcaggtga
     1681 ccgcgtaaac cgtcagttcg tggcagaacg
//
LOCUS       DAJTZL010000351         1686 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.354, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000351 DAJTZL010000000
VERSION     DAJTZL010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1686
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.354"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(784..1455)
                     /gene="queE"
                     /locus_tag="OO544_004315"
     CDS             complement(784..1455)
                     /gene="queE"
                     /locus_tag="OO544_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HCR6769737.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVISRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYDVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
     gene            1594..>1686
                     /locus_tag="OO544_004316"
     CDS             1594..>1686
                     /locus_tag="OO544_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6769738.1"
                     /translation="MSEENKENGFNHVKTFTKIIFIFSVLVFNDN"
BASE COUNT          551 a          343 c          301 g          491 t
ORIGIN      
        1 atcgcactct atcaattttc acttaaagta tgaactggat atctttattc ataagaacta
       61 ttcatcaaca gcgtatagag gcggttatgt aaaaccactc gttagcctca aaacaaagac
      121 aaatttccag atttattact taatcaatga ataacaagct taaaaaacaa actattaacc
      181 taaagaaaaa attaacagac attaattcaa tattaaaaaa ataatagatt aaaatttctt
      241 aacgatttaa gaatcataca aataacactt tgattaaatt taattttttg tatcgataat
      301 tgtgaacctc tctggcatgg agaactattt tgaacatgag gtgttacgtg gatattttgc
      361 ttattacaag tactgctaat ataaaaactt gagaaagaga taacgggtta tatggtggtt
      421 tatccccgct ggcgcgggga acactctaaa cataacctat tattaattaa tgatttttta
      481 agccagtcac aatctaccaa ctttacagta tcacacaaac aacacatcca ttatgttaaa
      541 gagtacttaa ttcattgatt aaaaaggtaa atatttaaaa taactctata caaattaaaa
      601 tctaccaaac tttaccgcaa taattttcac tccagcgaaa aattaatgcc acagaatttg
      661 tagagaatgc taatgattag ccctggatgg gtaaaatgga ggagttttca gaggcgacaa
      721 acaatataaa tgagtagaag tctttacagg ttccacccat ttataacgct tataaatgtt
      781 taatcaggca atatttagat atttatgtgt ttgcatcgac aaacgccaat tacgcgcaat
      841 gcaggtttca atgcacaaac gtgtggcatc atccttttgg ctaatcggct gcagtgcaat
      901 gactcgcggt ttatcatcgg tcagcgttgc caatagttca tccagtgctt caatatcgcg
      961 tacgcgcccc actggatgct tgatttcgtt ggctcgctcc agtgcctgtg acaacacgtc
     1021 atagccgccg cgcatgttca gctttggcga tacggtaacc caggtattcg gtgtgcagcg
     1081 tacctcatga gtaccactgg tttcgatctg gcagctaaaa ccgttctttt cgagcagatc
     1141 agtcagtggt agcaaatcat gaatgcaagg ctcaccaccc gtaatcacca catgccgcgc
     1201 ggtgtatccc tggcgactaa tgacagccaa caagtcttca ctgctcgccg ccccccactt
     1261 atcactctct ttggtcttcg ccagaatgct gaaaagggag acttcccgat cctcaagctt
     1321 ttcccaggtg tgtttggtgt cgcaccaggc acagccaacc gggcatccct gtaaacgaat
     1381 aaaaatggcg gggacgccgg taaagtaacc ctcaccttgc agggtctgga acatctcgtt
     1441 aatcgggtac tgcatagcat tctctgtgaa gtggataatt gttaattatt gcagatcctg
     1501 ccacaacaat catgtcttat taacattctg ttacaggcag atttaaagaa aaatgcgaaa
     1561 catatcgtta ataattaaag ggagtaacgt attatgtcag aagaaaataa agaaaatgga
     1621 tttaatcatg tcaaaacatt caccaaaatt atatttattt tttctgtatt agtttttaat
     1681 gataac
//
LOCUS       DAJTZL010000352         1677 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.355, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000352 DAJTZL010000000
VERSION     DAJTZL010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1677
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.355"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..889)
                     /locus_tag="OO544_004317"
     CDS             complement(<1..889)
                     /locus_tag="OO544_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6769739.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILQTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHP"
     gene            complement(989..1345)
                     /locus_tag="OO544_004318"
     CDS             complement(989..1345)
                     /locus_tag="OO544_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HCR6769740.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DCVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(1481..1588)
                     /gene="rprA"
                     /locus_tag="OO544_004319"
     ncRNA           complement(1481..1588)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="OO544_004319"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
BASE COUNT          538 a          334 c          392 g          413 t
ORIGIN      
        1 tgggatgcag gcagacggta aacagagcaa taaatgagat aaaggtgtac agcatcagta
       61 atgttgttgg gcgaacggta ttacgaatga gtggagcggt aataaataca caaagcagcg
      121 aacccacggt gtagatactg agtagtttga tcgacatagt gtatgacatg cctgcaacaa
      181 attgtccgta ctgtgccagc cactggctaa ccagataaaa cgttgccatt gagatatagc
      241 catataaggt ataactggct aaatcgataa ttgaacagcg atgttccgta gagctggtgg
      301 ttttctttat gacaggtaag cgacgacccg gatggggtgg gaacgtacaa cggtataaaa
      361 acagagcgtt aataaacata atgcctgcag caatcataaa ggaccaaccg aaccacagtt
      421 cagcccacac taacaggcta atgattagcg gtaataaaaa ttgtccgctg gaaacaaatg
      481 ctttaattaa aatattggct gtcccaggtg agcgtggaaa agcttccatc aaactgggat
      541 aagtgcctgc atcgagaaaa ctgtttgcca ttcccgccag aaagccaaaa acataagcga
      601 taatgatgtt attggtctgc aggatgccaa aaaagaaggc catatagcag cacatcccga
      661 gcatgataaa agggcggcga ccaaagcgat cggataataa tcctgcaaaa agcaagacac
      721 ttaatcgacc aatgcccagc gatgagataa ctatcgagac acccgcggca ttagtctgcc
      781 aaagtgtctc cagcgaggcc atattcaggc tcatcaaaat gacgcccata ccatgcacca
      841 ggtaattaaa atacaaccca agtgcggtag ggtaataggg atttttcata ggttctactt
      901 agcaaaggtg ctttaaaaag tgtagcgatg ttgtgtcagt atccattcat tattgttgta
      961 acaacgacaa ttcctttctg tggaaaaatt aacaaagccg ctccagatcg tgtgcatata
     1021 attcagcggc taccgattga tagatcttcc agtcaataaa attgccatca gggtagactt
     1081 gttgaaacgc ggttaagtcg gggaaaaaac gcatggtgtt gttgccgatg cggttattta
     1141 ttttctctat gatggcatga agatgttttt ttcgttgctg acgcatagcc aacagttgct
     1201 cgactacaca atcaggaatg gcgcatttgc cattttccca ctgacgccag ctttcactgt
     1261 caccggtctg ggcaatccag gtcgcgcatt catcaatagt catggcaaaa atatggcgga
     1321 gtgcctggag ttcataagcg ttcatcgtgt aatggctgca tgaaataaac aataaaatat
     1381 aaataacatt atcagcctgc tgacggcttg aagagagtca cagtatcttg tgcaacatta
     1441 ttggaaagag tgaggggcga ggtagcgaag cggaaaaatg ttaaaaaaaa gcccatcgtg
     1501 ggagatgggc aaagactaca cacagcaatt cgttgtttca ctcaggggat ttccatgctt
     1561 ataaatcaat atgttgattt ataaccgtga gctaatagta ggcatacgga ctttttgcgt
     1621 cgatcagatt cgtctcatta gtttaaaaac tgtaaagaat ttgcgacagg tactacc
//
LOCUS       DAJTZL010000353         1675 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.356, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000353 DAJTZL010000000
VERSION     DAJTZL010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1675
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.356"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..950
                     /locus_tag="OO544_004320"
     CDS             <1..950
                     /locus_tag="OO544_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCR6769741.1"
                     /translation="AELAVMLGRLERELRAGAVSAESEAWLAQCGLTAEQMAAQLEAE
                     YIPERKLHLYHCDHRGLPLALISPEGETAWQGEYDEWGNLLGETSAQHLQQSLRLPGQ
                     QYDEESGLYYNRNRYYDPLQGRYITQDPIGLRGEWNLYKYPLNPVRFIDSLGLKFHVN
                     GDPSDFNQAVEYLKQDSQMKETIDFLSSSEETINIEYIEGTNVRFNSNNMTIYWNSRA
                     SLFCSTELNSKSQSPALGLGHEFTHAQYCLLDKENFMALLSRTDKKYENKEEARVITI
                     IESRAAKTLGECTRGAHSGLPFYRVDGPLQTMKITGTPE"
     gene            962..1411
                     /locus_tag="OO544_004321"
     CDS             962..1411
                     /locus_tag="OO544_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005141515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6769742.1"
                     /translation="MMKYLMVLLSLFSGSVLGMGRVNELCGIDSVKTIEIINLPSYVT
                     TLVPLSKEGLNEIYRYKVVVNEIRDLYAGKIIDLLQMKYFRKEKYNNIRWGGSIISKG
                     NNKCEIYFDAFGECGSVNGINVCFEKNEMIGWIKKEIPLLSQKIGGL"
BASE COUNT          522 a          255 c          442 g          456 t
ORIGIN      
        1 cggcggagct ggcggtgatg ctggggaggc tggagcggga actgcgtgcg ggtgcggtga
       61 gtgcggagag tgaggcatgg cttgcgcagt gcgggctgac ggcggagcag atggccgcgc
      121 agctggaggc ggaatacatc ccggagagga aacttcatct ttaccactgc gaccaccggg
      181 gactgccgct ggcgctcatc agcccggaag gggaaacggc gtggcagggg gagtatgacg
      241 aatggggaaa cctgctgggc gaaaccagcg cgcagcacct ccagcagtca ctccgtctgc
      301 cggggcagca gtatgatgag gagtcggggc tgtactacaa ccgcaaccgg tactatgacc
      361 cgttgcaggg aaggtatatc acccaggatc cgatagggct gagaggtgaa tggaatctgt
      421 ataaatatcc attaaaccct gttcggttta tagattcttt gggtttaaaa ttccatgtta
      481 acggtgatcc aagtgatttt aatcaggctg ttgagtattt aaaacaagat tcacaaatga
      541 aagaaactat agatttttta tcaagctctg aagaaactat aaatatagag tatattgaag
      601 gaaccaatgt tagatttaat tcgaataata tgacgatata ttggaatagt agagcatctt
      661 tattttgttc aactgaatta aactcgaaat cacaatcgcc tgcattaggt ttgggacatg
      721 agtttactca tgcgcagtat tgccttcttg ataaggaaaa tttcatggct cttttaagcc
      781 gaacagataa gaaatatgag aataaagaag aggcaagggt tattacaata attgaatcgc
      841 gtgcggcgaa aactttaggt gagtgtacca gaggagctca tagtggactg cctttttatc
      901 gggtggatgg accgttgcag acgatgaaaa taacgggtac gccagaatag tgaggttttt
      961 tatgatgaaa tatttgatgg ttttattgtc tctattttct ggttcggtat tggggatggg
     1021 gcgagtgaat gaattgtgtg gaatagatag cgtgaaaacg atagagataa ttaatcttcc
     1081 atcgtatgtt accacgttag ttccgcttag caaggaaggt cttaatgaaa tatacagata
     1141 taaagtggtg gtgaatgaaa ttagagatct ttatgctggt aagataattg atttattgca
     1201 aatgaagtat ttcagaaaag aaaaatataa caatataaga tgggggggta gtatcatttc
     1261 gaaaggtaat aataaatgtg aaatttattt tgatgctttt ggtgaatgtg gaagtgtgaa
     1321 tggcattaac gtgtgttttg aaaaaaatga aatgataggt tggattaaaa aagaaatccc
     1381 tttgctgtcg caaaaaatag gtgggcttta aatggtaggg caatattcaa agacagtata
     1441 ggtaactatt tgtatggcca tgtaaggtaa aagaaaagga ataaaccatg agcggaaaac
     1501 cggcggcgcg gcagggcgac atgacccgca gggaaagatc atcagaagtt cactttttgt
     1561 actaaataat tcgcatttta tgtttaaaaa ttgagatatt ccttattacc taaagctgtt
     1621 ttttattgct tatacatgat caaatacttc ttacataatt aaggagaaca aaatg
//
LOCUS       DAJTZL010000354         1670 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.357, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000354 DAJTZL010000000
VERSION     DAJTZL010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1670
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.357"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(423..680)
                     /locus_tag="OO544_004322"
     CDS             complement(423..680)
                     /locus_tag="OO544_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6769743.1"
                     /translation="MSDHVIECASRAGRDFSEFMKGEKGMMEALASVDEFGEQLRLNG
                     CVNHHFVSYMMRNSIMQAFMDMAKAERKEERRRKRAEAKAK"
     gene            complement(677..1174)
                     /locus_tag="OO544_004323"
     CDS             complement(677..1174)
                     /locus_tag="OO544_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001496757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HCR6769744.1"
                     /translation="MKSLTLFNQPIRIGEDGMICLTDMWKASGKSESESPYHYLRNKQ
                     TKEFLAELEKNHESVVFTERGVHGGTYGGKFVAYDYAAWLNPGFKYAAYKVLDDYFTG
                     ELQHRNSLSAQLNMKCHEFDQKKDMASFCGQGLAAWRYTKPVLVAEINSLANQLQITI
                     PGLPG"
     gene            complement(1376..>1670)
                     /locus_tag="OO544_004324"
     CDS             complement(1376..>1670)
                     /locus_tag="OO544_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005760024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HCR6769745.1"
                     /translation="KRQRDVAELDARYIKELADANATIESLRADVSAGRKRLQVAATC
                     AKSTTGASGMGDGESPGLTADAELNYYRLRSGIDKITAQVNYLQEYIRTQCLK"
BASE COUNT          377 a          436 c          381 g          476 t
ORIGIN      
        1 atacagcgca tgatgtgtca ggcttgaata cctttatcct ttaaaaggga tatcagttaa
       61 gttatcccgt gtagggtata agccattatc aaagccactc tgtagggaat ggcttttgtg
      121 atggcatcac ttactcttta cgctgctatc ccactcatcc cggaattttg atgggttatt
      181 gaaaccttct gctgacataa caactccttc aatgtttggc tgaaattggg atgtctttcc
      241 atcagtccgc caccacaaag aatctttttt gccataaggc aggaggttca tctttcagtg
      301 gctgccggtg ttatttcccc acttactggc ttgggttgtt tcgtggtact gccgttaact
      361 ggtggcccag aataaattcc ggtttcatta tcaagcccac ccgtaaatgg gctttgtaat
      421 ggctacttcg cttttgcttc cgctcgctta cgccggcgct cttctttcct ctcggctttt
      481 gccatgtcca tgaatgcctg catgatcgag ttccgcatca tgtagctaac aaagtgatga
      541 ttgacacagc cgttgaggcg cagctgctcg ccaaactcat ccaccgaggc caatgcttcc
      601 atcatgccct tctcgccttt catgaactct gagaagtcgc gccccgctct ggaggcgcat
      661 tcaataacat gatcactcat cccggaagcc cggggatcgt aatctgcagc tggttagcca
      721 gggagttaat ctcagcgacc aacactggct tcgtatagcg ccatgctgcc agcccttgtc
      781 cgcagaagct cgccatatct ttcttctggt caaattcatg acatttcatg ttgagctgcg
      841 cacttaagct gttgcgatgc tgaagttctc cggtgaagta gtcatcgagg actttatagg
      901 ccgcgtactt gaacccgggg tttaaccaag ccgcataatc gtaagcaaca aacttcccgc
      961 catatgttcc accgtgtaca ccgcgctcag taaaaaccac agattcgtgg tttttctcca
     1021 gctcggctaa gaactctttg gtctgcttgt ttcgcaggta gtggtaaggc gattcagatt
     1081 cacttttacc actggctttc cacatatcag tgaggcagat catgccatct tcaccgatac
     1141 gaattggttg attgaagagg gttaatgatt tcatagcgtg tacctactct ttgaaatgaa
     1201 cctttgccgc acaggaaacc agcccaccga ggctcgccag cactaactgg tatcctcaaa
     1261 ggcccattcc aaaggggcag gttcggtgta aaaaacatgc gttgcggtac gcatttattg
     1321 caaaaagccc cgcatcgcga ggctcattaa atggactttg tgatttgcaa aaaaattatt
     1381 tcaggcattg cgtcctgatg tattcctgca ggtagttaac ctgcgcggtt atcttgtcga
     1441 ttccacttcg gagacggtaa taattgagtt cagcatctgc tgtaagtcct gggctttctc
     1501 catcgcccat gccgctggct ccggtcgttg actttgcaca ggtggcggcg acttgcaggc
     1561 gcttacgccc agcagaaaca tcagcacgga gactttcgat agtcgcgtta gcatcagcaa
     1621 gctcctttat gtatcttgcg tcgagttctg ctacatcacg ttgacgcttc
//
LOCUS       DAJTZL010000355         1663 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.358, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000355 DAJTZL010000000
VERSION     DAJTZL010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1663
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.358"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..702)
                     /locus_tag="OO544_004325"
     CDS             complement(<1..702)
                     /locus_tag="OO544_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HCR6769746.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISRLT
                     DAVSGDSLTAGEAPAALALSVDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDIKPVWALA
                     NDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHSNYGAALEKQGVEITLIYSGSHK
                     VDGNPYSHLPDDVRET"
     gene            complement(683..>1663)
                     /locus_tag="OO544_004326"
     CDS             complement(683..>1663)
                     /locus_tag="OO544_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCR6769747.1"
                     /translation="EDGYPGWMPQKWTWIPRELPGGRASFIHVFEPVEDGQTRGANVF
                     YSVMEQMKMLDTLQNTQLQSAIVKAMYAATIESELDTQSAMDFILGANSKEQRDRLTG
                     WIGEIAAYYAAAPVRLGGAKVPHLMPGDSLNLQTAQDTDNGYSVFEQSLLRYIAAGLG
                     VSYEQLSRNYAQMSYSTARASANESWAYFMGRRKFVASRQASQMFLCWLEEAIARRVV
                     TLPSKARFSFQEARSAWGNCDWIGSGRMAIDGLKEVQEAVMLIEAGLSTYEKECAKRG
                     DDYQEIFAQQVRETMERRAAGLKPPAWAAAAFESGLRQSTEEEKSDSRAA"
BASE COUNT          325 a          542 c          445 g          351 t
ORIGIN      
        1 tgtttcccgg acatcatccg gtagatggct gtaggggttg ccatccacct tatggctgcc
       61 gctgtaaatc agcgtgattt ccacgccctg tttctccagc gcagcaccgt aattactgtg
      121 agccatcatg acgccgatgg agcctgtccg ggcggtctgc gtgaccagac gtcgggaggc
      181 ggcgctggca agcaactgac ctgcactgca gttcatgtcg ttggccagcg cccataccgg
      241 ctttatgtca cgcacacggg cgatgatgtc agcgcagtca aatgcccctg ccaccatccc
      301 gcctggcgta tccatatcga gcagaatgcc gtccaccatc gggtcgctgg cagcctgttg
      361 cagacgggcg ataatgccgt tgtaaccggt catccccgaa tacggctgca gcgctcgcgt
      421 ccgactgacc agcgtgccgg acaccggcag cacggcgatg ccgttcatga cctgataact
      481 gcgggcctgt cgtggtccgt catcatcaac ggataacgcc agcgccgcgg gtgcctctcc
      541 ggcagtcagg ctgtcgccgg ataccgcatc cgtcaggcgg ctgatcccaa gctggcctgc
      601 aagcgcacaa aagaaaaccc gcgcataggc gggttcaagc atcagcggct cattaaaggc
      661 catactggca atatgcggga gattacgcag ctctgctgtc actcttctcc tcctctgttg
      721 attgtcgcag cccggattca aatgccgcag ccgcccaggc gggcggttta agaccggctg
      781 cacggcgctc catcgtttca cggacctgct gggcaaaaat ttcctgatag tcgtcaccgc
      841 gttttgcgca ctctttctcg taggtactca gtccggcttc tatcagcatc accgcttcct
      901 gtacttcttt cagaccatcg atggccatac gaccggagcc tatccagtcg cagttccccc
      961 aggcacttcg ggcttcctga aaactgaagc gcgcttttga aggtaacgtc accacgcggc
     1021 gagcgatggc ctcttccagc cagcacagaa acatctggct cgcctgacgg gatgcgacga
     1081 attttcgccg ccccataaag tacgcccacg actcgttcgc actggcccgt gccgtggagt
     1141 agctcatctg ggcgtaattc cgggaaagct gctcatacga gacacccagc ccggcagcga
     1201 tataccgcaa cagtgactgc tcaaacacgg agtagccgtt atccgtgtcc tgagccgtct
     1261 gcaggttcag tgagtccccc ggcatcaggt gcggcacttt tgcgcctccc agccggaccg
     1321 gtgctgcggc gtaatacgcg gcaatttcac caatccagcc ggtcagcctg tcccgctgct
     1381 ccttactgtt cgcgccaaga ataaaatcca tcgctgactg cgtatccagc tcactctcga
     1441 tggtggcggc atacatcgcc ttcacaatgg cgctctgcag ctgcgtgttc tgcagcgtgt
     1501 cgagcatctt catctgctcc atcacgctgt aaaacacatt tgcaccgcgg gtctgcccgt
     1561 cctccacggg ttcaaagacg tgaatgaacg aagcacgacc gcccggtaac tcacggggta
     1621 tccatgtcca tttctgcggc atccagccag gatagccgtc ctc
//
LOCUS       DAJTZL010000356         1663 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.359, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000356 DAJTZL010000000
VERSION     DAJTZL010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1663
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.359"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..702)
                     /locus_tag="OO544_004327"
     CDS             complement(<1..702)
                     /locus_tag="OO544_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HCR6769748.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISCLT
                     DAVSGDSLTAGEAPAALALSGDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDIKPVWALA
                     NDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHSNYGAALEKQGVEITLIYSGSHK
                     VDGNPYSHLPDDVRET"
     gene            complement(683..>1663)
                     /locus_tag="OO544_004328"
     CDS             complement(683..>1663)
                     /locus_tag="OO544_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCR6769749.1"
                     /translation="EDGYPGWMPQKWTWIPRELPGGRASFIHVFEPVEDGQTRGANVF
                     YSVMEQMKMLDTLQNTQLQSAIVKAMYAATIESELDTQSAMDFILGANSKEQRERLTG
                     WIGEIAAYYAAAPVRLGGAKVPHLMPGDSLNLQTAQDTDNGYSVFEQSLLRYIAAGLG
                     VSYEQLSRNYAQMSYSTARASANESWAYFMGRRKFVASRQASQMFLCWLEEAIVRRVV
                     TLPSKARFSFQEARSAWGNCDWIGSGRMAIDGLKEVQEAVMLIEAGLSTYEKECAKRG
                     DDYQEIFAQQVRETMERRAAGLKPPAWAAAAFESGLRQSTEEEKSDSRAA"
BASE COUNT          320 a          541 c          448 g          354 t
ORIGIN      
        1 tgtttcccgg acatcatccg gtagatggct gtaggggttg ccatccacct tatggctgcc
       61 gctgtaaatc agcgtgattt ccacgccctg tttctccagt gcagcgccgt aattactgtg
      121 agccatcatg acgccgatgg agcctgtccg ggcggtctgc gtgaccagac gccgggaggc
      181 ggcactggca agcagctgac ctgcgctgca gttcatgtca ttggccagcg cccataccgg
      241 ctttatgtca cgcacacggg cgatgatgtc agcgcagtca aatgctcccg ccaccattcc
      301 gcccggtgtg tccatatcga gcagaatgcc gtccaccatc gggtcgctgg cagcctgttg
      361 cagacgggcg ataatgccgt tgtaaccggt catccccgag tacggctgca gcgcccgcgt
      421 ccggctgacc agcgtgccgg acaccggcag cacggcgatg ccgttcatta cctgataact
      481 gcgggcctgt cgtggtccgt catcatcacc ggataacgcc agcgccgcgg gtgcctctcc
      541 ggcagtcagg ctgtcgccgg atactgcatc cgtcaggcag ctgatcccaa gctggcctgc
      601 aagcgcacaa aagaaaaccc gcgcataggc gggttcaagc atcagcggct cattaaaagc
      661 catgctggca atatgcggga gattacgcag ctctgctgtc actcttctcc tcctctgttg
      721 attgtcgcag cccggattca aatgccgcag ccgcccaggc gggcggttta agaccagctg
      781 cgcggcgctc catcgtttca cggacctgct gggcaaaaat ttcctgatag tcgtcaccgc
      841 gtttcgcgca ctctttctcg taggtgctca gtccggcttc tatcagcatc accgcttcct
      901 gaacttcttt cagaccatcg atggccatac gaccggagcc tatccagtcg cagttccccc
      961 aggcactgcg ggcttcctga aagctgaagc gcgcttttga aggtaacgtc accacgcggc
     1021 gaacgatggc ctcttccagc cagcacagaa acatctggct cgcctgacgg gatgcgacga
     1081 attttcgccg ccccataaag tacgcccacg actcgttcgc actggcccgt gccgtggagt
     1141 agctcatctg ggcgtaattc cgggaaagtt gctcatacga gacacccagc ccggcagcga
     1201 tataccgcaa cagtgactgc tcaaacacgg agtagccgtt atccgtgtcc tgagccgtct
     1261 gcaggttcag tgagtccccc ggcatcaggt gcggcacttt tgcgcctccc agacggaccg
     1321 gtgctgcggc gtaatacgcg gcaatttcac caatccagcc cgtcagcctt tcccgctgct
     1381 ccttactgtt cgcgcccaga ataaaatcca tcgctgactg cgtatccagc tcactttcaa
     1441 tggtggcggc atacatcgcc ttcacaatgg cgctctgcag ctgcgtgttc tgcagcgtgt
     1501 cgagcatctt catctgctcc attacgctgt aaaacacatt tgcaccgcgg gtctgcccgt
     1561 cctccacggg ttcaaaaacg tgaatgaacg aggcgcgccc gccgggtaac tcacggggta
     1621 tccatgtcca tttctgcggc atccagccag gatagccgtc ctc
//
LOCUS       DAJTZL010000357         1657 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.360, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000357 DAJTZL010000000
VERSION     DAJTZL010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1657
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.360"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            163..317
                     /locus_tag="OO544_004329"
                     /pseudo
     CDS             163..317
                     /locus_tag="OO544_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415954.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mRNA interferase"
     gene            363..779
                     /gene="hicB"
                     /locus_tag="OO544_004330"
     CDS             363..779
                     /gene="hicB"
                     /locus_tag="OO544_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
                     /protein_id="HCR6769750.1"
                     /translation="MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALL
                     TAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKVLLLNAFLQSEITQQELARRIGK
                     PKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV"
     gene            858..>1657
                     /locus_tag="OO544_004331"
     CDS             858..>1657
                     /locus_tag="OO544_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HCR6769751.1"
                     /translation="MKKYQQLAEQLREQIASGIWQPGDRLPSLRDQVALSGMSFMTVS
                     HAYQLLESQGYIIARPQSGYYVAPQAIKMPKAPVIPVTRDEAVDINTYIFDMLQASRE
                     PSVVPFASAFPDPRLFPLQQLNRSLAQVSKTATAMSVIENLPPGNAELRQAIARRYAL
                     QGITISPDEIVITAGALEALNLSLQAVTEPGDWVIVENPCFYGALQALERLRLKALSV
                     ATDVKEGIDLQALELALQDYPVKACWLMTNSQNPLGFTLTPQKKAQLVA"
BASE COUNT          436 a          383 c          409 g          429 t
ORIGIN      
        1 ttcagttttg caaattagcg caaagaaatt ctggaatctt ccttcctgat tttgcattgc
       61 attctgccgt tgcggcgatt tagtgctatt ttcgagcaaa ttacacatag gggtaaacac
      121 taatgtttac tttattgata tactcagcgg cagggaggcg atgtgaaaca aagcgagttc
      181 agacgttggc tcgaatctca gggcgtcgat gtagcgaatg gccgcaacca tttgaaactc
      241 aggtttcatg ggaggcgcag tgtcatgccg cgtcactcct gcgtaaagca atcctgaaac
      301 aactcggttt gagttaatcg ccaattaaaa aggttaatga catgcgagag acagtcgaaa
      361 ttatgcgtta tcccgtcact cttacacccg cgccggaagg cggttatatg gtttcttttg
      421 tggatatccc tgaagcgttg acgcagggcg aaactgtcgc tgaagcgatg gaagcggcaa
      481 aagatgcttt actgaccgca tttgattttt attttgaaga taacgagctt atcccgttac
      541 cttcgccatt aaatagtcat gatcacttta ttgaagtacc tttgagcgtc gcctctaaag
      601 tattgctgtt aaatgctttt ttacagtcag aaatcactca gcaagagtta gccaggcgaa
      661 ttggcaaacc taaacaggag attactcgcc tatttaactt gcaccatgcg acaaaaatcg
      721 acgccgtcca gcttgcggca aaggcgcttg gcaaagagtt atcgctggtg atggtttaat
      781 tacagttaac gaaaagttgt catttttaac aactgatata gactgccgaa tcatctgcac
      841 ataattacga ttcgataatg aaaaaatacc agcagcttgc agaacaatta cgcgagcaga
      901 ttgcgtcggg tatctggcaa cccggcgatc gtttgccttc gttgcgtgac caggtggcgc
      961 tttcaggcat gagctttatg actgtcagcc atgcctatca gttgctcgaa agtcagggat
     1021 atattatcgc acgaccgcag tcgggttatt acgttgcgcc acaggcaata aaaatgccga
     1081 aagcgccagt cattccagtc actcgagatg aagcagtcga tatcaacact tatatttttg
     1141 atatgttgca ggccagtcgc gaaccgtcgg tcgttccgtt tgcctcggcc tttcccgacc
     1201 cgcgactttt ccccctccaa caactaaacc gctcgctggc gcaggtaagc aaaaccgcca
     1261 cagcgatgag cgtgattgaa aacttaccgc caggaaacgc agaactgcgt caggctattg
     1321 ctcgtcgcta tgccttacag ggcatcacca tttctcctga tgaaattgtc attactgccg
     1381 gggcgttaga ggcattaaac ctcagtttgc aagcggtaac tgaaccgggc gattgggtga
     1441 tagtagagaa tccttgtttc tacggtgcgt tgcaggcgct ggagcggcta cggctgaagg
     1501 cgttatcggt ggcgacggat gttaaagaag ggatcgatct tcaggcgctg gaactggcgt
     1561 tgcaggatta tccggtgaaa gcgtgctggc tgatgactaa tagccagaac ccactcggat
     1621 ttaccttaac gccgcaaaaa aaagcacaac tggtggc
//
LOCUS       DAJTZL010000358         1557 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.361, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000358 DAJTZL010000000
VERSION     DAJTZL010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1557
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.361"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            271..1386
                     /locus_tag="OO544_004332"
                     /pseudo
     CDS             271..1386
                     /locus_tag="OO544_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312403.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          614 a          280 c          221 g          442 t
ORIGIN      
        1 aaaaaaatca cttcacacac tatcagagaa cataataact ttttaaaatg agcgcatttt
       61 gatagttgtt aacgatttcc tttacgatac tatcactacc ctttttttat acacaaatat
      121 taccgggtgg agataaaagg gaaatcaaag gtaattatat caggaagata taacgcatta
      181 catttattgt gtgtagaaga aaatccattc ctgttctaat tatccaattt aaacatctta
      241 gcataaaaca aatgatgaat aaggaatttt atgtcaattg actttacccc aggtataata
      301 aatacatatc acggcgatat ttataactgc acaacaaata ccgataatgc taaaacgcca
      361 gacacaccga agtggccttg cgataattgg gaagaacaac aacccattaa ttccacattc
      421 tctggagaag gatatatctc tgatcaatat gatttagtgc aacaccaact tcaacaaatt
      481 aatgcgtgcc acacgaacac tacatataca aatgcagact actcaaaagt tgtggctcaa
      541 cttgtaagtc ttattactaa cattgaaacg ataagttcga cacaacttac gcaacagaca
      601 caatcaatac ttaatcagat taataacatt agatatgaaa aaaacaaaag tgctgaatgt
      661 cgaatcattg ttgtcgctaa tcctaaaccc gacaaggcaa tcataactaa aattagcgta
      721 gaagagggaa tacccataac attcagtgtc caaaccatgt tttcagatac taattttatt
      781 gctgaacaac gagctgactt accaactaat ataaaagata ttcaaagcct gtatcaaaaa
      841 atgacgaagt tatatattga gcacagtgaa aataaaaaca ggatgaaagt ctttgccgga
      901 gccaacttta ttgatttcaa tatgacagga cagaacctgt ccgggttcgt tctaaccttg
      961 tcaaggtttt atttcgaaga tctacttaat ataaatttca ctgacgcaaa tttaggtgac
     1021 actatttttt acataaagaa caccctaccc ccaaattata taaagatgga caatatcttg
     1081 acaaacaaat cgaaggttta ttttcaacat tattaaccat taatgacaac tttttacgag
     1141 caaaagcgga aattgcttcg acaataatta aatttttaga ggccaggata accaatcttt
     1201 catacaatga catattaaaa tatcagcaag aattccaaaa acaatgttat aagcaagtta
     1261 aagcctttac gacactctct cgatacaata aaattcaaac atgggcagaa atgtctgaat
     1321 atcagttcga agtatttcaa tacgagacgc ttaaccccaa aaaaatgtca cacacgcctt
     1381 atttaaagag gccacttcct aatgaaaaag atataaatta tggggtggaa atagaaatac
     1441 catctggcaa aagaattcgc ttatcaaatc attatcagaa tataatacca taatattgat
     1501 tattagcact ttataattat tgaatgaaaa tagattttat gtaattttaa aacaaag
//
LOCUS       DAJTZL010000359         1516 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.362, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000359 DAJTZL010000000
VERSION     DAJTZL010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.362"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..>1516)
                     /locus_tag="OO544_004333"
     CDS             complement(<1..>1516)
                     /locus_tag="OO544_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCR6769752.1"
                     /translation="SRNGNITRWFYDSSRSGLPCAVEDGTGVRRRITRNRYGQLQAFT
                     DCSGYTTRYEYDRYGQQIAVHREEGISTYSSYNPRGQLVSQRDAQGRETRYEYSAAGD
                     LTAIVAPDGSRSEIQYDAWGKAVSTTQGGLTRSMGYDAAGRITVLTNENGSQSTFRYD
                     PVDRLTEQRGFDGRTQRYQYDLTGKLTQSEDEGLITLWHYDASDRITRRTVNGEPAEQ
                     WQYDDHGWLTEISHLSEGHRVAVHYGYDDKGRLTGERQTVETPETGEMLWEHETGHAY
                     SEQGLATRQEPDGLPPVEWLTYGSGYLAGMKLGGTPLVEYTRDRLHRETARSFGGEAY
                     ELATAWNTSGQLRSRHLNLPQLDRDYDWNDNGQLIRISGPQESREYRYSDTGRLTGVH
                     TTAANLDIDIPYATDPAGNRLPDPELHPDSTLTAWPDNRIAEDAHYVYRYDEYGRLAE
                     KTDRIPEGVIRMHDERTHHYHYDSQHRLVFYTRIQHGEPQVESRYLYDPLGRRTG"
BASE COUNT          222 a          510 c          442 g          342 t
ORIGIN      
        1 cccgtccggc ggcccagcgg gtcatagagg taccggctct ccacctgtgg ttcgccgtgc
       61 tgtatccgcg tgtagaacac caggcggtgc tggctgtcgt agtgatagtg gtgggtgcgc
      121 tcgtcgtgca tccggataac cccttccggg atacggtccg tcttctccgc cagcctgccg
      181 tattcatcgt agcgatagac atagtgcgca tcttccgcga tgcggttatc cggccacgcc
      241 gtgagggtgc tgtccgggtg cagttccggg tccggcagcc gatttcctgc cgggtctgtt
      301 gcatacggga tatcgatatc caggttcgct gccgtggtgt ggacgcccgt cagcctcccc
      361 gtgtcgctgt aacggtactc ccggctctcc tgcgggccac tgatgcggat tagctgtccg
      421 ttgtcgttcc agtcgtagtc acggtcaagc tgcggaaggt tcaggtgcct gctccggagc
      481 tggccgctgg tattccaggc ggtggcaagt tcatatgcct ccccgccgaa gctgcgggcc
      541 gtctcacggt gcagccggtc ccgcgtgtac tcgaccagcg gtgttccgcc cagcttcatc
      601 cccgcaagat aaccgctgcc atacgtcagc cactccaccg gcggcaggcc gtccggctcc
      661 tgacgggtcg ccagcccctg ttcgctgtac gcgtgccccg tctcatgctc ccacagcatc
      721 tcccccgtct ccggggtctc caccgtctgc cgttcgcccg tcagacggcc tttatcgtca
      781 tagccgtagt ggaccgccac acggtggcct tcactcaggt ggctgatttc tgtcagccag
      841 ccgtggtcgt catactgcca ctgctccgcc ggttcaccgt tcaccgtgcg ccgggtgata
      901 cggtccgacg catcgtaatg ccagagggtg ataagcccct catcttcact ctgcgtgagt
      961 tttccggtca ggtcatactg gtaacgttgc gtccggccgt caaagccgcg ctgctccgtc
     1021 agcctgtcga ccgggtcata ccggaacgtg gactggctgc cgttctcgtt ggtcagcaca
     1081 gtgatgcgcc cggcggcgtc ataccccatg cttcgcgtca gaccgccctg cgtggtgctg
     1141 acggcctttc cccacgcatc atactgtatc tcactgcggc tgccgtccgg ggcaacgata
     1201 gcggtgaggt ctcctgcggc gctgtactca taacgggttt cacgcccctg cgcatccctc
     1261 tgactgacca gttgaccgcg cgggttataa ctgctgtaag tgctgatgcc ttcttcccgg
     1321 tgaacggcga tttgttgacc gtaccggtca tactcatacc gcgtcgtgta ccccgagcag
     1381 tccgtaaacg cctgaagctg accgtaccgg tttcgcgtta ttctccgcct caccccggtt
     1441 ccgtcttcca ccgcacaggg caggccactg cgggaagaat cataaaacca gcgcgtaatg
     1501 ttaccgttgc gcgagg
//
LOCUS       DAJTZL010000360         1467 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.363, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000360 DAJTZL010000000
VERSION     DAJTZL010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1467
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.363"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>1467
                     /gene="istA"
                     /locus_tag="OO544_004334"
     CDS             <1..>1467
                     /gene="istA"
                     /locus_tag="OO544_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS21-like element ISSso4 family transposase"
                     /protein_id="HCR6769753.1"
                     /translation="CIGLTLHISASTVFEVLARFKASSLSWPLPADISHDTLEKLIFP
                     PKDTSASELVMPDMLYFDTEMRKPGVTRQLLWMEYKAQAGDKAMGYSHFCRCYRKWKK
                     TRRLSMRQEHRAGEKLFIDFCGPTVPVINPDTGEIRRVAIFVAVMGASNYTYVEACEG
                     QDMMSWLNAHSRCLTFLGGVPKLLIPDNLRSAVKKADRYEPVINDSYQALAEHYGTVI
                     IPARPRKPKDKPKAENGVLIVERWLLARIRNETFHTLRALNARLRELLTDMNNRPMKG
                     YGNQTRAERFRMLDAPALSPLPLEPYEYTEYKAVKVGPDYHVEYARHWYSVPHELVGQ
                     RLSLKVGQSVVQLWHKGQCVAQHPRSTHEYKHTTNPLHMPERHRRHGTWTPERLIEQG
                     NRTGPSTGRVVESMLKAKPHPELAYRAVLGLLALQKKYGPERLEKACYVALHYNAPDR
                     RFIDNLLRHHRDNVELPLSRQGEQHPAYASEHENLRGP"
BASE COUNT          360 a          421 c          382 g          304 t
ORIGIN      
        1 gttgtatcgg actgacgtta cacatcagcg cctcaaccgt ttttgaggtt cttgcccgct
       61 tcaaagcctc ttctctgtca tggccattac ctgcggatat atcccatgac acgctggaga
      121 aactcatttt cccgcccaaa gacacctctg cgtcagagct tgttatgcct gacatgctgt
      181 acttcgatac ggaaatgaga aaacctggcg tgacgcgcca actgttgtgg atggaataca
      241 aggcacaggc gggtgacaaa gcgatgggat actcacactt ctgccgttgc taccggaagt
      301 ggaaaaaaac gcgacgcctc tccatgcgac aggagcatcg tgctggcgaa aagctcttta
      361 tcgacttctg tggtcctacg gtccctgtta ttaatccgga caccggagag atacgccgcg
      421 tggcaatctt cgtggctgta atgggggcgt ccaactatac ctatgttgaa gcgtgcgaag
      481 gtcaggacat gatgtcatgg ttaaatgccc acagccgttg cctgaccttc ctgggcggcg
      541 taccgaagct gctcatcccc gataacctca gaagcgcagt gaaaaaagcc gatcgctatg
      601 agcctgtcat caacgacagt tatcaggcgc tggctgagca ctatggcacg gttattatcc
      661 cggcccgccc gcgcaaacct aaagataaac caaaagcaga aaacggcgtc ctcatcgtcg
      721 agcgatggct tcttgcccgc atccgtaatg aaaccttcca cacgctcagg gcgctgaatg
      781 cacgccttcg ggagttgctg acggacatga ataaccgacc aatgaaggga tacggtaatc
      841 aaacccgcgc agaacgcttc cgcatgctgg atgcaccagc actctctccg ctgcctctgg
      901 agccttatga atacacggaa tacaaagccg tgaaggttgg ccctgactac cacgttgaat
      961 atgcccgtca ctggtactcc gttccacacg aactggtcgg tcagcgtctg tcactgaagg
     1021 tggggcagtc ggtggtacag ctctggcaca aggggcagtg tgtggcgcaa catccgcgca
     1081 gcacacatga atataaacac acaaccaatc cgcttcacat gccagaacgc caccggcggc
     1141 atgggacatg gacaccggaa cgccttatcg agcagggaaa ccgtacaggt ccgtccacag
     1201 gccgggtggt tgaatccatg ctgaaagcca aaccccatcc ggagctggct taccgtgcag
     1261 tcctgggtct gctggcactt cagaaaaaat acggcccgga acggctggaa aaggcctgtt
     1321 acgttgccct gcactataac gccccggaca gacgctttat cgacaatctg ctgcgtcatc
     1381 atcgggataa cgttgagctt ccgttatccc gccagggcga acaacatccg gcatatgctt
     1441 cagagcatga aaacctgcgt ggtcctg
//
LOCUS       DAJTZL010000361         1463 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.364, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000361 DAJTZL010000000
VERSION     DAJTZL010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1463
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.364"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..106
                     /locus_tag="OO544_004335"
     CDS             <1..106
                     /locus_tag="OO544_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR6769754.1"
                     /translation="FISPRIADRITDGGRNWLSFNWPSYRSCIRGVAA"
     gene            103..>1463
                     /locus_tag="OO544_004336"
     CDS             103..>1463
                     /locus_tag="OO544_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCR6769755.1"
                     /translation="MTTPVWRNDDLEGAVIGAFFLRGADHEVMDILITLPADIFSVRA
                     YRDIYTGICRQARVSGVIDPVLLCNEMPELAPVITDTGRKTWVKSSLEHYVAALRRNA
                     ALRDAEKTLNEALQKLRDAHTCEAAEDALKDAQNMMVTLSTGKGVIQPVHIDDVLPEV
                     VERVECRNQGLEKSRTLMTGIDELDAKTGGMEPGDLVFIAARPSMGKTELALDIIDKV
                     TEQGHGVLLFTMEMANIQIGERMVSAAGGMPVSRLKSVAHFEDEDWARFSQGVGRMTG
                     RNIWMVDQANLTIDEICATTKHHLIKHPETALVVVDYLGLIKTRTTGRHDLAVGEISK
                     GLKGLAKSGGFPLIALSQLSRGVESRPNKRPMNSDLKNSGEIEADADIILMLYRDEVY
                     NPDTQATGIAEINITKQRNGSLGTIYRRFYNGHFLPVDQESAQVLSTPMRQPQPRRYS
                     NTRT"
BASE COUNT          366 a          345 c          430 g          322 t
ORIGIN      
        1 gttcatcagc ccccgcattg ccgacaggat cacagacggg gggcgcaact ggttgtcgtt
       61 caactggccc agctaccgtt cttgtatcag aggtgtggct gcatgacaac accagtctgg
      121 cgtaatgatg acctggaagg cgctgtcatt ggcgcattct ttctgcgtgg ggcagatcat
      181 gaagtgatgg atattctgat cacactaccg gcggacattt tttctgtacg agcgtatcgg
      241 gatatctaca caggcatctg cagacaggcc cgtgtttcag gagtgattga ccccgtgctg
      301 ttgtgtaatg agatgccgga acttgccccg gtgattactg ataccgggcg taaaacctgg
      361 gtgaagtctt cactggagca ctatgttgca gcgttgcggc gcaatgccgc actgcgcgat
      421 gcagaaaaaa cactgaatga ggcgctgcag aaattacgtg atgcgcatac ctgtgaagca
      481 gctgaagatg ccctgaagga tgcgcagaac atgatggtca cactgtcgac aggaaagggc
      541 gtcattcagc cggtacatat tgatgatgtg cttccggaag tggttgagcg tgttgaatgc
      601 cggaatcagg ggctggagaa atccaggacg ttgatgaccg gtattgatga actggacgca
      661 aaaacaggcg gtatggagcc cggagacctg gtattcattg ccgcccgtcc ttcgatgggg
      721 aaaaccgaac ttgcgctgga catcatcgac aaggtgactg agcaggggca tggtgtgctt
      781 ctgttcacca tggagatggc gaacatccag attggtgaac gtatggtgtc tgcggctgga
      841 gggatgccag tatcacgcct gaaatctgtg gctcactttg aagatgaaga ctgggcgcgt
      901 ttctcacaag gggtggggcg gatgaccggg cgcaatatct ggatggtgga ccaggcgaac
      961 ctgaccattg atgagatatg cgcaacaacg aaacaccatc tgattaaaca tccggaaacg
     1021 gcgctggtgg tggttgatta tctcgggctg ataaaaaccc gaaccacggg gcgtcatgac
     1081 cttgccgtgg gtgaaatctc aaaggggctt aaaggcctgg caaaatccgg tggttttccg
     1141 ttgattgcgc tgagccagct ctcccgcggt gtggagtcca gacccaataa acgtcccatg
     1201 aactcagacc tgaaaaattc cggagaaata gaggcggatg cagacatcat tctgatgctt
     1261 tacagggatg aagtgtacaa cccggataca caggctacag gcatagcaga aattaatatc
     1321 acgaaacaac gtaacggttc tctggggacg atttaccggc gtttttataa cggacatttt
     1381 ctgcctgtgg accaggaaag cgcacaggtt ctttccaccc caatgcggca gccccagccg
     1441 cgcagataca gcaatacacg tac
//
LOCUS       DAJTZL010000362         1461 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.365, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000362 DAJTZL010000000
VERSION     DAJTZL010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1461
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.365"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            502..957
                     /gene="csgB"
                     /locus_tag="OO544_004337"
     CDS             502..957
                     /gene="csgB"
                     /locus_tag="OO544_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin minor subunit CsgB"
                     /protein_id="HCR6769756.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSTQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
     gene            998..1450
                     /gene="csgA"
                     /locus_tag="OO544_004338"
     CDS             998..1450
                     /gene="csgA"
                     /locus_tag="OO544_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin major subunit CsgA"
                     /protein_id="HCR6769757.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGNHGGGGNNSGPNSEL
                     NIYQYGGGNSALVLQTDARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSATL
                     DQWNGKNSEMTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNNATAHQY"
BASE COUNT          446 a          275 c          337 g          403 t
ORIGIN      
        1 aatgatagaa aagttgtaca tttggttttt attgcacaat tttaaaaaat catacaaatg
       61 gtgataactt actaataatg catataaaaa atatttcggt gtagtccttt cgtcatgtaa
      121 aaagttgttg ttttttctcc acacctccgt ggacaaattt ttactccaaa aagacgaggt
      181 ttgtcacggc ttgtgcgcaa gacatatcgc agcaatcagc gatgggcaag aagaatgact
      241 gtctggtgat ttttgatagc ggaaacggag atttaaaaga aaacaaaata ttttttgcgt
      301 agataacagc gtatttacgt ggcttttaat actttggtat gaacaaaaaa agaaaaatac
      361 aacgcgcggg tgagttatta aaaatatttc cgcagacata ctttccatcg taacgcagcg
      421 ttaacaaaat acaggttgcg ttaacaacca agttgaaatg atttaatttc ttaaatgtac
      481 gaccaggtcc agggtgacaa catgaaaaac aaattgttat ttatgatgtt aacaatactg
      541 ggtgcgcctg ggattgcagc cgcagcaggt tatgatttag ctaattcaga atataacttc
      601 gcggtaaatg aattgagtaa gtcttcattt aatcaggcag ccataattgg tcaagctggg
      661 actaataata gtactcagtt acggcaggga ggctcaaaac ttttggcggt tgttgcgcaa
      721 gaaggtagta gcaaccgggc aaagattgac cagacaggag attataacct tgcatatatt
      781 gatcaggcgg gcagtgccaa cgatgccagt atttcgcaag gtgcttatgg taatactgcg
      841 atgattatcc agaaaggttc tggtaataaa gcaaatatta cacagtatgg tactcaaaaa
      901 acggcaattg tagtgcagag acagtcgcaa atggctattc gcgtgacaca acgttaattt
      961 ccattcgact tttaaatcaa tccgatgggg gttttacatg aaacttttaa aagtagcagc
     1021 aattgcagca atcgtattct ccggtagcgc tctggcaggt gttgttcctc agtacggcgg
     1081 cggtaaccac ggtggtggcg gtaataatag cggcccaaat tctgagctga acatttacca
     1141 gtacggtggc ggtaactctg cacttgttct gcaaactgat gcccgtaact ctgacttgac
     1201 tattacccag catggcggcg gtaatggtgc agatgttggt cagggctcgg atgacagctc
     1261 aatcgatctg acccaacgtg gcttcggtaa cagcgctact cttgatcagt ggaacggcaa
     1321 aaattctgaa atgacggtta aacagtttgg tggtggcaac ggtgctgcag ttgaccagac
     1381 tgcatctaac tcctccgtca acgtgactca ggttggcttt ggtaacaacg cgaccgctca
     1441 tcagtactaa tacatcattt g
//
LOCUS       DAJTZL010000363         1457 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.366, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000363 DAJTZL010000000
VERSION     DAJTZL010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1457
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.366"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..1010)
                     /gene="cadA"
                     /locus_tag="OO544_004339"
     CDS             complement(<1..1010)
                     /gene="cadA"
                     /locus_tag="OO544_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HCR6769758.1"
                     /translation="MNVIAILNHMGVYFKEEPIRELHRALERLNFQIVYPNDRDDLLK
                     LIENNARLCGVIFDWDKYNLELCEEISKMNENLPLYAFANTYSTLDVSLNDLRLQISF
                     FEYALGAAEDIANKIKQTTDEYINTILPPLTKALFKYVREGKYTFCTPGHMGGTAFQK
                     SPVGSLFYDFFGPNTMKSDISISVSELGSLLDHSGPHKEAEQYIARVFNADRSYMVTN
                     GTSTANKIVGMYSAPAGSTILIDRNCHKSLTHLMMMSDVTPIYFRPTRNAYGILGGIP
                     QSEFQHATIAKRVKETPNATWPVHAVITNSTYDGLLYNTDFIKKTLDVKSIHFDSAWV
                     PYT"
     gene            complement(1090..1437)
                     /locus_tag="OO544_004340"
     CDS             complement(1090..1437)
                     /locus_tag="OO544_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HCR6769759.1"
                     /translation="MTALMILITLMNSAGGKASDLFGELTGIAVLLTMLPYFYSCVDL
                     IRFEGVNIRNFVSLICSVLGCVFCFIALMGASSFELAGTFIVSLIILMFYARKMHERQ
                     SHSMDNHTASNAH"
BASE COUNT          406 a          320 c          364 g          367 t
ORIGIN      
        1 gtgtaaggca cccacgcgga gtcaaagtgg atggatttca catccagtgt tttcttgatg
       61 aagtcggtgt tgtacagcag accatcatag gtagagttgg taattacagc atgtaccggc
      121 caggttgcgt ttggtgtttc tttcacgcgc ttagcaatgg tagcgtgctg gaattcactc
      181 tgtgggatac caccaagaat accgtaagcg ttacgggtcg ggcggaaata gattggcgta
      241 acatcgctca tcatcatcag gtgggtcagc gatttgtggc agttacggtc aatcagaatg
      301 gtgctgcctg ccggagcaga gtacatgcca acaattttgt tcgcagtgga agtaccgttg
      361 gtcaccatgt agctgcggtc tgcgttaaag acgcgagcga tatactgttc tgcttctttg
      421 tgtggaccac tatgatccag cagagaaccc agttcagata ctgaaatgga aatatcagat
      481 ttcatggtat tcggaccaaa gaaatcatag aacaggctac ctaccgggct tttctggaat
      541 gcagtaccgc ccatgtgacc aggagtacag aaagtatatt taccttcacg aacatattta
      601 aacagtgctt tagtcagcgg aggcagaata gtgttgatat attcgtcagt ggtctgcttg
      661 atcttattag caatatcttc agcagcaccc agcgcatatt caaagaagct aatctgtaaa
      721 cgcaggtcat tcaggcttac atcgagagtg gaatacgtat tagcgaacgc gtacaacggc
      781 aggttctcgt tcattttgct aatttcttcg cacagctcga gattatattt atcccagtca
      841 aaaataacgc cgcacagacg cgcattgttt tcgatcagtt ttaataagtc gtcacggtcg
      901 ttcgggtaaa caatctggaa gttcagacgt tcaagcgcgc gatgaagttc acggatgggt
      961 tcttctttaa aataaacccc catgtgattc aatattgcaa taacgttcat agtcatatct
     1021 ccaggtaaaa aaggcccctc ccaacacatg ggacaaaatg aaaggaggag cctcggaaaa
     1081 tacttttaat taatgtgcgt tagacgcggt gtggttatcc attgagtggc tctggcgctc
     1141 gtgcattttg cgagcgtaga acatcaggat aatcaggctg acgatgaagg tacctgccag
     1201 ctcgaaggag cttgcgccca tcagcgcgat gaagcagaac acacaaccca gtacagagca
     1261 gatcaggctg acaaagttgc ggatgttaac gccttcaaaa cgaatcaggt caacgcaaga
     1321 gtagaagtac ggcagcatag tcagcagtac tgcgataccg gtcagttcac cgaacaggtc
     1381 agatgcttta ccaccggcag agttcatcag agtgatgagg atcatcaggg cagtcatttt
     1441 cactgcagcc agcagca
//
LOCUS       DAJTZL010000364         1430 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.367, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000364 DAJTZL010000000
VERSION     DAJTZL010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1430
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.367"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            13..438
                     /locus_tag="OO544_004341"
     CDS             13..438
                     /locus_tag="OO544_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000056357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCR6769760.1"
                     /translation="MLRIIHTKGVSLPDDRESVFWLNIKNIPPSASNKATNSLEIAVK
                     TRIKLFWRPANIRLIPEDAAPKVKWRREGRNLIAENPNPIHISVMDVIVDGHDVPLNM
                     IRPFETLTLPLPANSAGGQMTWRFINDYGAVSDPIKMTL"
     gene            463..>1430
                     /locus_tag="OO544_004342"
     CDS             463..>1430
                     /locus_tag="OO544_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR6769761.1"
                     /translation="MMTTRIVVGLTAGTCLIFSQNLMAEVSVFNPALLEIDHQSGVDI
                     RQFNRANLMPPGVYSVDIFINGKMFERQDVTFVQDNPDADLHACFIAIKKTLSSFGIK
                     VDALKSFNDVDETVCLDPAPRIEGSSWQFDSDKLQLNISIPQIYMDAMAYDYISPTRW
                     DEGINALTINYDFSGSHTLRSDYGSQETDTSYLNLRNGLNIGPWRLRNYSTLNTSDGR
                     AEYNSISTWIQRDIAALRSQIMIGDTWTASDIFDSTQIRGARLYTDNDMLPASQNGFA
                     PVVRGIAKSNATVIIRQNGYVIYQSAVPQGAFEITDLNTASTGGDL"
BASE COUNT          386 a          331 c          341 g          372 t
ORIGIN      
        1 tcgcaagaaa aaatattacg cattattcac accaaaggtg tgtcattgcc tgatgatcgc
       61 gagtcagtct tctggctgaa tattaaaaat attccgccat cagcgagcaa caaagcgacg
      121 aactcgctgg aaatagcagt aaaaacgcgt atcaagctgt tctggcgacc agcaaatatc
      181 cgccttattc ccgaagatgc tgcgccgaaa gtgaaatggc gccgggaagg gcgaaatctg
      241 atagcagaga atcccaaccc aattcatatc agtgtgatgg atgtcatcgt tgatggccat
      301 gatgtgcccc tcaatatgat ccgtcctttt gaaacgctca cgttaccttt acctgctaat
      361 tctgcgggag gccaaatgac ctggcgattt attaatgatt acggcgcagt cagtgacccg
      421 ataaaaatga cgctgtaagc aagggataag gataggcgta ttatgatgac gaccagaata
      481 gtggttggcc tcacggcagg gacgtgtctg attttctcgc aaaacctgat ggccgaggtc
      541 agtgtattca atccggcgct tctggaaatc gaccatcaat ccggagtcga tattcgccag
      601 tttaatcggg caaacctgat gcccccaggt gtttatagcg ttgatatttt tatcaacggt
      661 aaaatgtttg aacgtcagga tgtgacattt gttcaggata atccagatgc tgatctgcac
      721 gcttgcttta ttgccattaa aaaaacactg tcctcctttg gcataaaagt tgatgcgctc
      781 aaatcgttca atgatgtgga tgagacggtt tgcctcgatc ctgctccacg tattgaaggc
      841 tcatcctggc agtttgacag tgataaattg cagctgaata tatccattcc ccaaatctac
      901 atggacgcga tggcttatga ttacatcagc cccacgcgtt gggatgaggg gattaacgcg
      961 ctcaccatca actacgattt ttctggttca catacactac gttcagatta tggttcacaa
     1021 gagacagata ccagttatct caatctgcgc aatggactga atattggacc gtggcggcta
     1081 cgtaattaca gtactttaaa caccagcgat ggccgtgcgg aatacaactc cattagtacc
     1141 tggatacagc gcgatattgc cgcgttaaga agccagatta tgattggtga tacgtggacg
     1201 gcgagcgata ttttcgacag tacgcaaatt cgcggcgcgc gtttgtatac tgataacgat
     1261 atgctacccg ccagccagaa tggctttgct cctgtggttc gtgggattgc aaagtccaac
     1321 gccaccgtca tcattcggca gaatggctac gtgatttatc agtctgccgt tccacaaggt
     1381 gcttttgaga tcaccgatct caacaccgcc agtacaggtg gcgatttgga
//
LOCUS       DAJTZL010000365         1426 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.368, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000365 DAJTZL010000000
VERSION     DAJTZL010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1426
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.368"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..118)
                     /locus_tag="OO544_004343"
     CDS             complement(<1..118)
                     /locus_tag="OO544_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein C"
                     /protein_id="HCR6769762.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQH"
     gene            complement(130..822)
                     /gene="rutB"
                     /locus_tag="OO544_004344"
     CDS             complement(130..822)
                     /gene="rutB"
                     /locus_tag="OO544_004344"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HCR6769763.1"
                     /translation="MTTLTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            complement(822..>1426)
                     /locus_tag="OO544_004345"
     CDS             complement(822..>1426)
                     /locus_tag="OO544_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HCR6769764.1"
                     /translation="GDFFTMNDCRVSPQPSVPMKVICAGQSDAGMAFSAQYADFNFCF
                     GKGVNTPTAFAPTAVRMKQAAEQTGRDVGSYVLFMVIADETDDAARAKWEHYKAGADE
                     EALSWLTEQSQKDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPG
                     AEGVLLTFDDFLSGIETFGERIQPLMQCRAHLPALTQEVA"
BASE COUNT          323 a          397 c          396 g          310 t
ORIGIN      
        1 tatgttgatc aaaagccagc gtaccggaga catacaccac gccatcagcc agcgtgccgg
       61 gaacgaaggg ggccagcggt gcgctgctgc cagcgggaat aattacggat tttggcatcg
      121 ttaaactcct taagcgatac gagcaaagga cgtgggagaa agcgcgtcgc agaacgtttc
      181 gacgtcgctg acccagccaa aaaaggtttc gatattgaac aacgcggctt tctgtgcaaa
      241 ttcaggcccc gcctggtgtg ttgcgtcttc cagcaccacg ccgaaatact ccagaaaaaa
      301 gccatcgcgt agcgtcgatt cgacgcagac gttggtagcg ataccggtga aaaccagatg
      361 gcgtattcca cggctgcgca aaatgctgtc cagcggcgta ttgaagaaac cgctgtagcg
      421 cggcttcggc agcacaatat cgccaggctg cggcaccagt tcatccacca gttgataatc
      481 ccaggagcct ttcgccagca atttcccctg cagctgcggc tgcttacgca tggttttcag
      541 ggcgttcgat ttatgaaaat tcggtgagcc gggtccgcca gcttcgacat actgttcatc
      601 ccagccattt tgaaaccaga tgatcagcat ccctgccgct cgcgctgcgg tcacggcggt
      661 ttgaatgttg gcaatgaccg ggcgagtggt tgagacatca aacccggcga gatctaagta
      721 gccgcctggc gtggcataag cgttttgcat atccaccacg atcagcgcac tttgctgcgg
      781 atcgaaggta atggcttccg gtcgagcggt taaggtcgtc atcatgccac ctcctgagtc
      841 agcgcaggga gatgggcgcg gcactgcatc agtggttgaa tgcgctcgcc gaaggtttcg
      901 attcccgaca gaaaatcatc gaaggtcaac agcacgcctt cggcaccagg cacgcttgcg
      961 acttcatcta acatgcgcgc gacactggcg taagaaccga ctaatgtccc catattgatg
     1021 tttaccgccg aagtgggatc ggccatctga cggacgttgg tgtcagtacc ggagcgggta
     1081 tctttctgac tttgttcggt tagccagctt aacgcctctt catccgcgcc cgctttgtag
     1141 tgttcccatt tggcgcgagc ggcatcgtcg gtttcatcgg caatcaccat aaacaacaca
     1201 taagagccaa cgtcgcgccc ggtttgctct gcggcctgtt tcatccgcac agcggtcggg
     1261 gcgaaagccg tgggtgtatt tacgcctttg ccgaaacaga agttgaaatc ggcatactga
     1321 gcggagaacg ccatgccagc gtcgctttgc ccggcgcaga tcactttcat ggggacactc
     1381 ggttgcggac tgacgcgaca atcattcatg gtgaaaaaat cgcctt
//
LOCUS       DAJTZL010000366         1420 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.369, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000366 DAJTZL010000000
VERSION     DAJTZL010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1420
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.369"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>1420
                     /gene="rhsC"
                     /locus_tag="OO544_004346"
     CDS             <1..>1420
                     /gene="rhsC"
                     /locus_tag="OO544_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rhs element protein RhsC"
                     /protein_id="HCR6769765.1"
                     /translation="VLTTQAQRAEEARQQAISGGTEPSAFPDTLPGYTEYGRDNGIRL
                     SAVWLTHDPEYPENLPAAPLVRYGWTPRGELAAVYDRSNTQVRSFTYDDKYRGRMVAH
                     RHTGRPEIRYRYDSDGRVTEQLNPAGLSYTYQYEKDRITITDSLNRREVLHTQGEAGL
                     KRVVKKEHADGSVTQSQFDAVGRLRAQTDAAGRTTEYSPDVVTGLITRITTPDGRASA
                     FYYNHHSQLTSATGPDGLEMRRKYDESGRLIQETAPDGDIIRYRYDNPHSDLPCATED
                     ATGSRKTMTWSRYGQLLSFTDCSGYVTRYDHDRFGQMTAVHREEGLSQYRAYDSRGQL
                     IAVKDTQGHETRYEYNAAGDLTTVIAPDGSRNGTQYDAWGKAICTTQGGLTRSMEYDA
                     AGRVIRLTSENGSHTTFRYDVLDLLIQETGFDGRTQRYHHDLTGKLIRSEDEGLVTHW
                     HYDEADRLTHRTVNGETAERWQ"
BASE COUNT          338 a          421 c          436 g          225 t
ORIGIN      
        1 tggtactgac cacgcaggcg cagcgggcag aagaagcccg gcagcaggcc atttccggcg
       61 ggacggaacc gtccgctttt cctgataccc tgccgggtta caccgaatat ggccgggaca
      121 acggcatccg tctgtctgcc gtgtggctga cgcacgaccc ggaatacccg gagaatttac
      181 ctgccgcgcc gctggtgcgc tatggctgga cgccgcgcgg cgaactggcg gcagtgtatg
      241 accgcagcaa tacacaggtg cgcagcttta cttacgatga taaataccgg ggccggatgg
      301 tggcgcaccg tcacacgggc cggccggaaa tccgttaccg ttacgacagc gacgggcggg
      361 tgacagaaca gctaaacccg gcaggcttaa gctacacgta tcagtatgag aaggaccgca
      421 tcaccatcac cgacagcctg aaccgccgtg aagtgctgca cacgcagggt gaagccgggc
      481 tgaagcgggt ggtgaaaaag gaacacgcgg acggcagcgt cacgcagagt cagtttgatg
      541 cggtgggcag gctcagggca cagacggatg ccgcaggccg gacgactgag tacagcccgg
      601 atgtggtgac gggactcatc acgcgcatca ccacgccgga tggcagggca tcggcgtttt
      661 actataacca ccacagccag ttaacgtcag ccaccgggcc tgacgggctg gaaatgcgcc
      721 gcaaatatga tgaatccggc cgtctgattc aggaaactgc ccctgacggc gatatcatcc
      781 gctaccgtta tgataatcca cacagtgact taccctgcgc aacggaagat gccaccggca
      841 gccggaaaac catgacgtgg agccgttacg gtcagttgct gagcttcacc gactgttccg
      901 gttatgtaac ccgttatgac catgaccgct tcgggcagat gacggcggtg caccgcgagg
      961 aagggctgag tcagtaccgc gcatacgaca gccgtggaca gttaattgcc gtgaaagaca
     1021 cgcagggcca tgaaacgcgg tatgaataca acgccgccgg tgacctgacc accgtcattg
     1081 ccccggacgg cagcagaaac gggacacagt acgatgcgtg gggaaaagct atctgtacca
     1141 cgcagggcgg tctgacgcgc agtatggaat acgatgctgc cggacgggtc atccgcctga
     1201 ccagtgaaaa cggcagccac accaccttcc gttacgatgt actcgacctg ctgatacagg
     1261 aaaccggctt tgacggccgc acacagcgtt atcaccacga cctgaccggc aaacttatcc
     1321 gcagcgagga tgaggggctg gtcacccact ggcactatga cgaagcagac cgcctcacgc
     1381 accgcaccgt gaatggcgaa accgcagagc ggtggcagta
//
LOCUS       DAJTZL010000367         1414 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.370, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000367 DAJTZL010000000
VERSION     DAJTZL010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1414
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.370"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            211..1407
                     /locus_tag="OO544_004347"
     CDS             211..1407
                     /locus_tag="OO544_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCR6769766.1"
                     /translation="MTESSDYESVQVFIGVDVGKDTHHAVAINRSGKRLFDKALPNDE
                     NKLRSLISDLKQHGQILLVVDQPATIGALPVAVARSEGVLVGYLPGLAMRRIADLHAG
                     EAKTDARDAAIIAEAARTLPHALRTLKLADEQIAELSMLCGFDDDLAAQTTQASNRIR
                     GLLTQIHPALERVLGPRLEHPAVLDLLQRYPSPEKLASLGEKKLAAQLCKLAPRLGKR
                     LAADIAQALAEQTVVVPGTNAAAVVLPRLALQLITLRKQRDEVALEVEQRVLAHPLYP
                     VLTSMPGVGVRTAARLLTEVACRAFASAAHLAAYAGLAPVTRRSGSSIRGEHPSRRGN
                     KALKRALFLSAFAALRDPLSRAYYTRKMSQGKRHNQALIALARRRCDVLFAMMRDGTF
                     YTPQGS"
BASE COUNT          297 a          439 c          378 g          300 t
ORIGIN      
        1 ccttataatg gggtgttagc cagccagacc cggcatgatt actgccccca gtcgtccatg
       61 atccgggggg tgatgtcacc gggtctggtg gggcgctggt aaccgctaat aggggtcagg
      121 tcaggcactt ttgccgggac cgtctgtaac gtggatgccg gtacctgctc cccgtggtta
      181 tctggttaac ccatatacaa gggagacaga atgaccgaat ccagcgatta cgaatccgtc
      241 caggtcttta tcggcgttga tgtcggtaaa gatacgcatc acgctgttgc cattaatcgt
      301 tcaggtaaac gcctgttcga taaagcatta cccaacgacg aaaacaaact caggtcgcta
      361 atatctgacc tgaaacaaca tggtcagata ctgctggttg ttgatcagcc agctaccatc
      421 ggtgcgttac ctgtcgccgt tgcccgctca gaaggagtcc ttgtcggata cctccctgga
      481 ctggccatgc gccgcatagc cgacttacac gccggtgaag ctaaaactga tgctcgtgac
      541 gctgccatca ttgccgaagc tgcccgtacc ctgcctcacg cgctacgcac gctgaaactg
      601 gctgacgagc aaatcgccga actctccatg ctctgcggct tcgatgatga tcttgccgca
      661 cagacaacgc aggccagcaa ccgtatccgc ggccttctga cccagataca tccggcactg
      721 gagcgcgttc tcggtccgag acttgagcac ccggcggtac tcgatcttct ccagcgatat
      781 ccctcaccag aaaaactcgc ttcgctgggt gagaagaagc tggcagccca gctctgcaaa
      841 cttgcgcctc gtctgggtaa acgccttgca gcagacatag ctcaggcact ggccgaacaa
      901 accgtcgtcg ttcccggcac gaatgccgct gccgtagtac tgccacgtct ggcactccag
      961 ctcatcacgc tgcgtaagca aagagacgag gtggcgcttg aggtagaaca gcgagttctt
     1021 gctcaccctc tttacccggt cctgaccagt atgcccggag tcggtgtcag gaccgcagcc
     1081 agactcctca ccgaggtcgc ctgccgcgcc ttcgcctctg ccgcacatct cgctgcttat
     1141 gctggccttg cgccggtaac tcggcgatcc ggctcgtcaa tacgcggtga gcatccctcg
     1201 cgacggggta ataaagctct caaacgggcg ttgttcctgt cggccttcgc cgcgctcagg
     1261 gatccgctct ccagggctta ctacacccgc aaaatgagtc agggaaaacg acacaatcag
     1321 gcgcttatcg ccctggcgag acgacgctgc gacgttctgt tcgccatgat gcgcgacggg
     1381 actttttata ccccgcaggg gtcataacat gctt
//
LOCUS       DAJTZL010000368         1392 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.371, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000368 DAJTZL010000000
VERSION     DAJTZL010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1392
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.371"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..459
                     /locus_tag="OO544_004348"
     CDS             <1..459
                     /locus_tag="OO544_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR6769767.1"
                     /translation="LNAMASQRTKEARGGELQINELLPASLDAAKAHGLTERDVYEAT
                     DYYKTPRGQQPGGATKMLFSHAQKTLAWDAFAFTEVLLTQPVMVVVGEKVGAFGAYRD
                     GLEVYGRAMVSQDRQLVSLPDFSHYELYDKPEAVQEALEKVIPFFNTHLG"
     gene            477..1115
                     /locus_tag="OO544_004349"
     CDS             477..1115
                     /locus_tag="OO544_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR6769768.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSKIIMGDVLNHAALKQAMQGQDVVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFGEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPVTDGDKPLFM"
BASE COUNT          444 a          282 c          307 g          359 t
ORIGIN      
        1 ttgaatgcaa tggcgtcgca acgaaccaaa gaggctcgtg gtggagaatt acagatcaac
       61 gaattacttc ccgccagcct ggatgcagca aaagcccatg gactgaccga acgcgatgtt
      121 tatgaagcaa ctgactacta taaaacacca cgcgggcaac aaccgggagg tgcgacaaaa
      181 atgttgttct cacatgcaca gaaaacgctg gcctgggatg ccttcgcatt tactgaagtg
      241 ttactgactc agccggtgat ggtagtagtt ggtgaaaaag tcggtgcctt cggtgcttat
      301 cgtgatggac tggaagtcta tggccgggcg atggtttcgc aggatcgtca gttagtttca
      361 ttacccgatt tctcacatta tgaactgtat gacaaaccgg aagcagttca ggaggcgctg
      421 gaaaaagtta ttcctttttt caacacccat ctgggataag tttcatgaga aatattatga
      481 aaaatgtact gattcttggt gctggcggtc agatagcccg ccatgtgatt aatcaactgg
      541 cagataaaca aaccattaaa caaactttat ttgcccgtca gccagcaaaa atccacaaac
      601 cctatccaac taatagcaag attattatgg gagatgtact gaatcatgca gcactgaagc
      661 aggccatgca gggacaggac gttgtgtatg ccaaccttac gggggaagat ttagatattc
      721 aggcaaattc agtgatcgct gcgatgaaag cctgtgatgt taaacgtctg atctttgttc
      781 tttccctcgg tatttatgat gaagttccag gaaaatttgg cgaatggaat aatgcagtta
      841 ttggtgagcc cctaaaacca ttccgcaggg ctgcggatgc aattgaggct tctggactgg
      901 aatatacaat tcttcgccct gcctggctga ctgatgaaga tattattgat tatgagctta
      961 cctcacgcaa tgaaccattc aaaggtacga tcgtatcacg taagagtgtc gcagcattaa
     1021 tcacagatat catcgataaa cctgagaaac acattggtga gaatataggt atcaatcaac
     1081 ccgtgactga tggtgacaag ccattattca tgtaaaccaa ttaaatagtt accatcgtaa
     1141 tgtactatgt gcagtacgat ttattaaact tattaaggtg aactgatcaa tccgatgcga
     1201 ttaacatgtg tagaaccagt aagaataaaa tacgccatgt atataacatt acgacttaca
     1261 tctaagtcag cataattcta atatctgcac atcaataaaa atgcaaacaa cttccggcaa
     1321 aaaaagtgat acaagatcga tgacatagcg ttgaagaaaa ggatttgata atccgcaaaa
     1381 tttgcacaac ac
//
LOCUS       DAJTZL010000369         1390 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.372, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000369 DAJTZL010000000
VERSION     DAJTZL010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1390
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.372"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>1390
                     /locus_tag="OO544_004350"
     CDS             <1..>1390
                     /locus_tag="OO544_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HCR6769769.1"
                     /translation="NYYLPKESAYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARV
                     GYSKMLLGVYAYFIEHKQRNTLIWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYGK
                     KHRDNTLTMKRFSNGRGFWCLGGKAAKNYREKSVDVAGYDELAAFDEDIEQEGSPTFL
                     GDKRIEGSVWPKSIRGSTPKVRGTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDK
                     ETPFGLKWTPDDPSSVFYLCEHNACVIRQQELDFTDARYICEKTGIWTRDGILWFSSS
                     GEEIEPPDSVTFHIWTAYSPFTTWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIG
                     ERPDAEVMAERKEHYSAPVPDRVAYLTAGIDSQRDRYEMRVWGWGPGEESWLIDRQII
                     MGRHDDEQTLLRVDEAINKTYTRRNGAEMSISRICWDIGGIDPTIVYERSKKHGLFRV
                     IPIKGASVYGKPV"
BASE COUNT          330 a          341 c          408 g          311 t
ORIGIN      
        1 aattactatc tcccgaaaga atccgcatac caggaagggc gctgggaaac actgcccttt
       61 cagcgggcca tcatgaatgc gatgggcagc gactacatcc gtgaggtgaa tgtggtgaag
      121 tctgcccgtg tcggttattc caaaatgctg ctgggtgttt atgcctactt tatagagcat
      181 aagcagcgca acacacttat ctggttgccg acggatggtg atgccgagaa ctttatgaaa
      241 acccacgttg agccgaccat ccgcgatatt ccgtcgctgc tggcgctggc tccgtggtat
      301 ggcaaaaagc accgggataa cacgctcact atgaagcgtt tttccaatgg tcgtggcttc
      361 tggtgcctgg gcggtaaagc ggcaaaaaac taccgtgaaa agtcggtgga tgtggcgggt
      421 tatgatgaac ttgctgcctt tgatgaggat attgaacagg aaggctctcc gacgttcctt
      481 ggcgacaaac gtattgaagg ctcggtctgg ccaaagtcca tccgtggctc cacgcccaaa
      541 gtgagaggca cctgccagat tgagcgtgca gccagtgaat ccccgcattt tatgcgtttt
      601 catgttgcct gcccgcactg cggggaggag cagtatctta aatttggcga caaagagacg
      661 ccgtttggcc tcaaatggac gccggatgac ccctccagcg tgttttatct ctgcgagcat
      721 aatgcctgcg tcatccgcca gcaggagctg gactttactg atgcccgtta tatctgcgaa
      781 aagaccggga tctggacccg tgatggcatt ctctggtttt cgtcatccgg tgaagagatt
      841 gagccacctg acagtgtgac ctttcacatc tggacagcgt acagcccgtt caccacctgg
      901 gtgcagattg tcaaagactg gatgaaaacg aaaggggata cgggaaaacg taaaaccttc
      961 gtaaacacca cgctcggtga gacgtgggag gcgaaaattg gcgaacgtcc ggatgctgaa
     1021 gtgatggcag agcggaaaga gcattattca gcgcccgttc ctgaccgtgt ggcttacctg
     1081 accgccggta tcgactccca gcgggatcgc tacgaaatgc gcgtatgggg atgggggccg
     1141 ggtgaggaaa gctggctgat tgatcggcag attattatgg gccgccacga cgatgaacag
     1201 acgctgctgc gtgtggatga ggccatcaat aaaacctata cccgccggaa tggtgcagaa
     1261 atgtcgatat cccgtatctg ctgggatatt ggcgggattg acccgaccat tgtgtatgaa
     1321 cgctcgaaaa agcatgggct gttccgggtg atccccatta aaggggcatc cgtctacggt
     1381 aagccggtgg
//
LOCUS       DAJTZL010000370         1376 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.373, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000370 DAJTZL010000000
VERSION     DAJTZL010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1376
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.373"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            3..983
                     /locus_tag="OO544_004351"
     CDS             3..983
                     /locus_tag="OO544_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001346030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HCR6769770.1"
                     /translation="MVGVPVPDNIHPMIKDEAGVWSWRTPVLKGNLYEYFFNVDGVRS
                     IDIGTAMTKPQRQVNSSMVLVPGSYLDTRSVAHGDLIAITYHSNALQSERQMYVWTPP
                     GYTGMGEPLPVLYFYHGFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPDTETDA
                     KGIIPEDFVPQERRKVFYPLNAKAADRELMNDIIPLISKRFNVRKDADGRALAGLSQG
                     GYQALVSGMNHLESFGWLATFSGVTTTTVPDEGVAARLNDPAAINQQLRNFTVVVGDK
                     DVVTGKDIAGLKTELEQKKIKFDYQEYPGLNHEMDVWRPAYAAFVQKLFK"
     gene            998..>1376
                     /locus_tag="OO544_004352"
     CDS             998..>1376
                     /locus_tag="OO544_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCR6769771.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMY
                     EYLTTLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGL"
BASE COUNT          359 a          315 c          363 g          339 t
ORIGIN      
        1 tagtggtggg tgttccggtt ccggacaata ttcacccgat gatcaaagac gaagcaggag
       61 tctggtcgtg gcgcacgcct gtcctgaaag gcaacctgta tgagtatttt ttcaatgttg
      121 atggtgtacg cagcattgat ataggcaccg caatgactaa gcctcagcgc caggttaact
      181 ccagtatggt tctggtgcca ggcagttatc tggatacgcg ttctgttgcg catggtgatt
      241 tgatcgccat aacttaccac tccaacgcat tgcaatctga acgtcagatg tatgtctgga
      301 ccccgccagg atacaccggc atgggcgagc ctttgccagt gctctatttc tatcacggtt
      361 ttggtgatac cggacgttcc gctatcgatc aggggcgtat cccgcaaatc atggataacc
      421 tgctggctga agggaaaatt aaaccgatgc tggtggtgat cccggatacc gaaaccgatg
      481 cgaagggcat tattcccgaa gatttcgtgc ctcaggaaag acgtaaagtc ttttatccgc
      541 tgaatgctaa agcggcagat cgcgaactga tgaacgatat tatcccgctg attagcaagc
      601 gttttaatgt ccgtaaagat gccgatggtc gcgcgctggc agggctttca caaggcgggt
      661 accaggcgct ggtttccgga atgaatcatc tggaaagctt tggctggctg gccacattca
      721 gtggtgttac cacgacaacc gtaccggatg aaggtgtcgc ggcccggctg aacgatccgg
      781 cagctattaa ccagcaacta cgtaatttta ctgtggtcgt tggtgataaa gatgtcgtaa
      841 ccggcaagga tatcgccggg ctgaaaactg agcttgagca gaaaaaaatt aagtttgatt
      901 accaggaata tcccggcctg aaccatgaaa tggatgtctg gcggcctgcc tatgcagcct
      961 tcgtacagaa attatttaaa taaaataagg cattaagatg aaattaatca ttaccgaaga
     1021 ttaccaggaa atgagccgtg tcgcggcaca ccatctgctt ggttatatgt cgaagacgcg
     1081 tcgtgttaac ctggcaatta ccgccggtag cacgcccaaa ggcatgtatg aatacctcac
     1141 caccctggtt aaaggtaagc cctggtacga taactgctat ttctataatt tcgatgaaat
     1201 tccctttcgc ggcaaagagg gagaaggcgt aacgattacc aatctgcgta atctgttttt
     1261 cacccctgcg gggatcaaag aagagaatat ccagaagctc actattgata actaccgcga
     1321 gcatgatcag aaactggcgc gtgaaggcgg cctggatttg gtggtgctgg gattag
//
LOCUS       DAJTZL010000371         1344 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.374, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000371 DAJTZL010000000
VERSION     DAJTZL010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1344
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.374"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..477)
                     /gene="umuC"
                     /locus_tag="OO544_004353"
     CDS             complement(<1..477)
                     /gene="umuC"
                     /locus_tag="OO544_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HCR6769772.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGMGIAQTKTLAKLANHAAKKWQRQ"
     gene            complement(477..896)
                     /locus_tag="OO544_004354"
     CDS             complement(477..896)
                     /locus_tag="OO544_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HCR6769773.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            1269..>1344
                     /locus_tag="OO544_004355"
     CDS             1269..>1344
                     /locus_tag="OO544_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin activation protein"
                     /protein_id="HCR6769774.1"
                     /translation="MTEIVADKTVEVVKNAIETADGALD"
BASE COUNT          405 a          321 c          277 g          341 t
ORIGIN      
        1 ctgccgctgc catttttttg ccgcatgatt ggcaagctta gccagcgttt tggtctgggc
       61 gatccccata ccaacagtaa gatgggtacg ttgtagcacc gttgcgcgaa tttctctgcc
      121 aaaatcagtc agatcgcgac aattacgcac acctgtcaga tcgcagaatg cctcatcaat
      181 actgtaaatc tcgacgcggg gcgatagttc ttccagcgtc gacatcaccc gattgctcat
      241 gtctgcgtaa agctcataat tgctgctaaa gcaaaccaca ccacagcgac gaaacagatc
      301 tttttgtttg aaccagggat cgcccatttt aacacccagc gcctttgcct cagcgtttcg
      361 ggcgataacg caaccgtcat tattcgatag cacaaccacc ggtttacccc ataaatcagg
      421 gcgaaacacc gtctcacagc tggcataaaa cgcgtttaca tcacagagag caaacatcag
      481 cgcatcgcct taacgacgtg gatcaccaca ccaaagacat ccagcgtatc ttcgctactg
      541 atggtaatgg gcgagtacgc actgttcatg ggaataagct gtaccgtcgg gcgtagttgc
      601 aattttttca ccgtaaactc gccgtcaaca gcagcgatga caatatcacc atggctggcg
      661 gtaatagcgc tatcgacaat cagtaaatca ccgtcactaa ttccaccatc aatcatagaa
      721 tcaccacttg ctttgacgaa gtaagtcgcg ctgggatgct ggatcaacag ttgattcaga
      781 tcgatgcgct gttcgacgta atctgctgcc ggtgaaggaa agccacactg aacaagatcg
      841 ctaaatagcg gaaaagtcac aatttcgcgg agatccgcag gcttgataaa caacataata
      901 atctgcctga agttatactg tttttatata cagtaatctg ttcttgccag cagatcaata
      961 ctgattcagg ctatcaatat ttgtcgctgc atagactgct gatttttcgt tctcttatcc
     1021 tgtgctcacg tggccttctg gcgacgacgc tcatccagca gaaatgaaaa atatcacccg
     1081 gcaaaaaaat agaatagaag cattcgccat aatgacatta aacattgttt gatatttatc
     1141 atattaatag aaataaagac attgacgcat cccgcccggc taactatgaa ttagatgaag
     1201 taaaatttat taatagttgt aaaacaggag tttcattaca atttatatat ttaaagaggc
     1261 gaatgattat gactgaaatc gttgcagata aaacggtaga agtagttaaa aacgcaatcg
     1321 aaaccgcaga tggagcatta gatc
//
LOCUS       DAJTZL010000372         1341 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.375, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000372 DAJTZL010000000
VERSION     DAJTZL010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1341
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.375"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..184
                     /locus_tag="OO544_004356"
     CDS             <1..184
                     /locus_tag="OO544_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6769775.1"
                     /translation="TISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLAQ
                     GKRRCQQQFTSQEVQN"
     gene            194..>1341
                     /gene="yicI"
                     /locus_tag="OO544_004357"
     CDS             194..>1341
                     /gene="yicI"
                     /locus_tag="OO544_004357"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HCR6769776.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPG"
BASE COUNT          335 a          318 c          351 g          337 t
ORIGIN      
        1 cacgattagc atcattattg cgctattcac gattgttccg gcgatctgtt atttgctgag
       61 cgcgattatc gctaaacgct actactcact cacgacgcac aatctgaaaa ccgttatgga
      121 acagctggct cagggtaaac gccgttgcca gcaacaattc acctctcaag aagtgcagaa
      181 ctaaggaacg gcaatgaaaa ttagcgatgg aaactggttg attcaacctg gcctcaattt
      241 gattcacccg cttcaggtgt tcgaggttga acagcaggat aatgaaatgg tggtctatgc
      301 tgccccccgt gatgtgcgtg aacgtacctg gcagcttgat acgcctttat ttacgctgcg
      361 ctttttctcc ccacaggaag gtattgtcgg tgtgcggatt gagcattttc agggggcgct
      421 gaataacggt cctcattatc cgctcaatat tttgcaggac gtgaaggtca caatcgaaaa
      481 cacagaacgt tatgctgagt ttaaaagtgg caacttaagc gcgcgtgtca gcaaaggtga
      541 gttctggtca ctggattttc tgcgcaacgg cgaacgtatt accggtagtc aggtgaaaaa
      601 taatggctac gtgcaggaca cgaataatca acgcaattat atgtttgagc ggcttgatct
      661 tggcgttggc gaaacagttt acggtctggg agagcgcttt actgccctgg tgcgcaatgg
      721 ccagacggta gagacctgga accgggacgg cggcacaagt actgaacagg cgtataaaaa
      781 tatcccgttc tacatgacta accgtggtta tggggtactg gtcaatcatc cccagtgtgt
      841 ctcttttgaa gtgggatcgg agaaagtctc caaagtgcag ttcagcgttg agagtgaata
      901 tctcgaatac tttgttatcg acggcccgac gccgaaagcg gtacttgatc gttatacccg
      961 ctttactggt cgtccggcgc tgccgcccgc gtggtccttc ggcctgtggc taaccacttc
     1021 atttaccacc aactacgacg aagcgacggt aaacagcttt atcgatggta tggcggaacg
     1081 caatctgccg ctgcatgttt tccactttga ctgtttctgg atgaaagcct tccagtggtg
     1141 cgattttgag tgggacccgc tgactttccc tgacccggaa gggatgatcc gccgcctgaa
     1201 agcgaaaggg ctgaaaatct gcgtctggat taacccctat atcggtcaaa aatcccccgt
     1261 ctttaaagag ttacaagaga aaggctattt actcaaacgc ccggacggtt cgctatggca
     1321 gtgggataaa tggcagccag g
//
LOCUS       DAJTZL010000373         1322 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.376, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000373 DAJTZL010000000
VERSION     DAJTZL010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1322
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.376"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..1322
                     /gene="rhsB"
                     /locus_tag="OO544_004358"
                     /pseudo
     CDS             1..1322
                     /gene="rhsB"
                     /locus_tag="OO544_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="rhs element protein RhsB"
BASE COUNT          320 a          388 c          403 g          211 t
ORIGIN      
        1 ataccctgcc gggttacacc gaatatggcc gggacaacgg catctgtctg ccgtgtggct
       61 gacgcacgac ccggaatacc cggagaattt acctgccgcg ccgctggtgc gctatggctg
      121 gacgccacgc ggcgaactgg cggtggtgta tgaccgtagt ggcaaacagg tgcgcagctt
      181 tacttacgat gataaatacc ggggccggat ggtggcgcac cgtcacacgg gccggccgga
      241 aatccgttac cgttacgaca gcgacgggcg ggtgacagaa cagcttaacc cggcaggctt
      301 aagctacacg tatcagtatg agaaggaccg catcaccatc accgacagcc tgaaccgccg
      361 tgaagtgctg cacacgtagg gtgaagccgg gctgaagcgg gtggtgaaaa aggaacacgc
      421 ggacggcagc gtcacgcaga gtcagtttga cgccgtgggc aggctcagga cacagacgga
      481 tgccgcaggc cggacaacag aatacagccc ggatgtggtg acgggcctca tcacgcgcat
      541 caccacgccg gatggcaggg catcggcgtt ttactataac caccacagcc agttaacgtc
      601 agccaccggg cctgacgggc tggaaatgcg ccgcaaatat gatgaatccg gccgtctgat
      661 tcaggaaacc gcccctgacg gcgatatcac ccgctaccgt tatgataatc cacacagtga
      721 cttaccctgc gcaacggaag atgccaccgg cagccggaaa accatgacat ggagccgtta
      781 cggtcagttg ctgaccgtca ctgactgttc cggttatgta acccgttatg accatgaccg
      841 ttttggtcag gtgacggcgg tgcaccgcga ggaagggctg agccagtacc gcgcatacga
      901 cagccgtgga cagttaattg cggtgaaaga cacgcagggc catgaaatgc ggtatgaata
      961 caacgccgcc ggtgacctga ccgccgtcat tgccccggac ggcagcagaa acgggacaca
     1021 gtacgatgcg tggggaaaag ccatctgtac cacgcagggc gggctgacgc gcagtatgga
     1081 atacgatgct gccggacggg tcatccgtct gaccagtgaa aacggcagcc acaccacctt
     1141 ccgttacgat gtactcgacc ggctgataca ggaaaccggc tttgacggcc gcacacagcg
     1201 ttatcaccac gacctgaccg gcaaacttat ccgcagcgag gatgaggggc tggtcaccca
     1261 ctggcactat gacgaagcag accgcctcac gcaccgcacc gtgaagggtg aaaccgcaga
     1321 gc
//
LOCUS       DAJTZL010000374         1275 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.377, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000374 DAJTZL010000000
VERSION     DAJTZL010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1275
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.377"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>1275
                     /locus_tag="OO544_004359"
     CDS             <1..>1275
                     /locus_tag="OO544_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HCR6769777.1"
                     /translation="SSKTAAASSASSAASSASSASASKDEATRQASAAKGSATTASTK
                     ATEAAGSATAAAQSKSTAESAATRAETAAKRAEDIASAVALEDASTTKKGIVQLSSAT
                     NSTSESLAATPKAVKAAYDLANGKYTAQDATTAQKGIIQLSSATNSTSETLAATPKAV
                     KAANDNAEKRLQKDQNGADIPGKDTFTKNIGACRAFGGSVSTTTGNWTTAQFIEWLDS
                     QGAFNHPYWMCKGSWSYGNNKIITDTGCGNIHLAGAVIEVMGIKSAMTIRITTPTTSS
                     GGGTTNAQFTYINHGTDYSPGWRRDYNSRNKPTASEIGALPSGGTAVSSVNLASKGRV
                     AALTDNTQGAAGLELYEVYNNGYPTAYGNIIHLKGMTAVGEGELLIGWSGTSGAHAPA
                     FIRSRRDTTDANWSPWAQLYTSAHPPAEFYPVG"
BASE COUNT          359 a          324 c          358 g          234 t
ORIGIN      
        1 atcctcaaaa acggctgccg catcgtccgc cagttcggcg gcgtcatcgg catcatcggc
       61 gtctgcttca aaagatgagg cgaccagaca ggcgtcagca gcgaagggca gcgccacgac
      121 ggcatccacg aaggcgacag aggctgctgg tagtgcgacg gcagcagctc agagcaaaag
      181 tacggcggaa tctgcagcaa cgcgcgctga gacagcggca aaacgggcag aggatattgc
      241 atccgccgtg gcgcttgagg atgcgagcac gacgaaaaag gggatagtac agctcagtag
      301 tgcgactaac agcacttccg agtcactggc ggcaacacca aaagccgtta aggccgcgta
      361 tgacctggct aacgggaaat acaccgcaca ggatgcaacg acagcacaga aagggataat
      421 ccagctaagc agcgcgacca acagcacgtc tgaaacgctg gcggcaacgc caaaggcagt
      481 aaaagcagcc aatgacaatg ctgagaaacg tctgcagaaa gatcagaacg gtgcggatat
      541 ccctggcaaa gacaccttta cgaaaaatat tggtgcctgc cgtgccttcg gtgggtcagt
      601 aagcacaaca acaggaaact ggacgactgc acagtttatc gagtggctgg attctcaggg
      661 agcatttaac catccatact ggatgtgcaa gggttcctgg tcttatggca ataataaaat
      721 cattactgat actggctgcg gtaatattca tctcgccgga gctgtcattg aagtaatggg
      781 gataaagtca gcgatgacga tccgcattac cactccgacc acatccagcg gtggcggaac
      841 aaccaacgcc cagtttacct atattaatca cggaacagat tattcacctg gctggcgaag
      901 ggactataac tccagaaata agccaacggc atcagagatc ggggcgctac cgtcaggtgg
      961 aacagcagta tcatcagtta atctggcttc aaaaggtcgg gtagccgcgc tgacagacaa
     1021 tacgcagggg gcagcaggtc ttgagttata cgaggtgtat aacaacggat atccaacagc
     1081 gtatggaaat atcattcacc tgaaagggat gacagccgtt ggcgaaggtg agttactcat
     1141 cggctggagt ggtacaagcg gtgctcatgc tccggcattt attcgttcac gacgggatac
     1201 gaccgacgca aactggtcgc cgtgggcgca gctttacacc tcggctcatc ctcctgcaga
     1261 gttttatcca gtcgg
//
LOCUS       DAJTZL010000375         1269 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.378, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000375 DAJTZL010000000
VERSION     DAJTZL010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1269
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.378"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(176..307)
                     /locus_tag="OO544_004360"
     CDS             complement(176..307)
                     /locus_tag="OO544_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HCR6769778.1"
                     /translation="MIAYCFCEWHCSSRIMSVLADGVLVAGVVVVAFPLLKKKTPDC"
     gene            complement(807..883)
                     /locus_tag="OO544_004361"
     tRNA            complement(807..883)
                     /locus_tag="OO544_004361"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(847..849),aa:Arg,seq:tct)
     gene            complement(984..1059)
                     /locus_tag="OO544_004362"
     tRNA            complement(984..1059)
                     /locus_tag="OO544_004362"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1024..1026),aa:Ile,seq:cat)
     gene            complement(1162..>1269)
                     /locus_tag="OO544_004363"
     CDS             complement(1162..>1269)
                     /locus_tag="OO544_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCR6769779.1"
                     /translation="YDALVTRFDIEEAWAERQLKKVTRLIFPESVVNSS"
BASE COUNT          383 a          344 c          233 g          309 t
ORIGIN      
        1 ttcagtacca gcacctgaat ccgcgattat cccatatacc tactcgctga ttgcccctcc
       61 gcacaggggg attcaccatg ccagtttctt ttaacaaact ccccgcaaac cagacaacag
      121 tcaaccgcct gaattgtgaa gtatttaaaa atttctcccg ctaactgata cccggctaac
      181 agtctggcgt tttctttttc agcaacggga aagcaacaac caccacaccc gccaccagca
      241 caccgtcagc cagcactgac attatccggc tgctgcaatg ccattcacaa aaacagtaag
      301 caatcacttt ttaccgtaac aggtgataat ccagatatgt atctacccca gatgagtaat
      361 ccgaagttca tccataccac aggtcctggc tattctgttg tactcctgaa caagagcaaa
      421 taattctgaa ttagcaacca tgaactcatc gcaaaccctc tgtatagcat cactattcag
      481 aataataacg tctcttcccg aaagacgatc aggagtacag aacaaaactg tcaaacggct
      541 gaaggccttt gctcgtgctg cattgactat atcaatacgc tgcctaagga tgaaacaccc
      601 cgacgcctca tcaatattca ctctacccac accatatgaa tgataaatat ttaatgctga
      661 aaaaaccatt agaccgtata acaaacactc aatcaatact taacagaact tttatttttg
      721 acaaacataa aatattttca acaatatcct gagccaggta tatttcagta taaggctctg
      781 ccggaaggaa tctggaagaa tgaatatggc gcgctgtact ggattcgaac cagtgaccga
      841 ttgcttagaa ggcaattgct ctgtccggct gagctaacaa cgctgaatac cgataatgga
      901 ccgccatcgg ggacccgccc ccgcaccaac aaccctgtta tcgtgtcgtc tgctcttcct
      961 gataagctaa tggcggtttg tgatggtggc ccttgctgga tttgaaccag cgacctggcg
     1021 attatgagtc gctcgctctc accactgagc taaagggccg ggagcagaat aataacggtc
     1081 cgtaattaat tccgcaataa aaaacccgct cggcggcggg ttgtagaaac tcttctaacg
     1141 tcaggcataa aacgcccata gttatgacga atttaccaca gattccggaa aaatcaacct
     1201 agttaccttt tttaactgcc gctcagccca tgcttcttca atatcaaacc gagtcaccag
     1261 cgcatcata
//
LOCUS       DAJTZL010000376         1268 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.379, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000376 DAJTZL010000000
VERSION     DAJTZL010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1268
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.379"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..348)
                     /locus_tag="OO544_004364"
     CDS             complement(<1..348)
                     /locus_tag="OO544_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HCR6769780.1"
                     /translation="MFDGELSFALKLAREMGRPDWRAMLAGMSSTEYADWHRFYSTHY
                     FHDVLLDMHFSGLTYTVLSLFFSDPDMHPLDFSLLNRREADEEPEDDVLMQKAAGLAG
                     GVRFGPDGNGVIPT"
     gene            complement(330..752)
                     /locus_tag="OO544_004365"
     CDS             complement(330..752)
                     /locus_tag="OO544_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HCR6769781.1"
                     /translation="MFLKTESFEHNGVTVTLSELSALQRIEHLALMKRQAEQAESDSN
                     RKFTVEDAIRTGAFLVAMSLWHNHPQKTQMPSMNEAVKQIEQEVLTTWPTEAISHAEN
                     VVYRLSGMYEFVVNNAPEQTEDAGPAEPVSAGKCSTVS"
     gene            complement(768..>1268)
                     /locus_tag="OO544_004366"
     CDS             complement(768..>1268)
                     /locus_tag="OO544_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HCR6769782.1"
                     /translation="SFTLAWMPGEQGQQALLAWFNEGDTRAYKIRFPNGTVDVFRGWV
                     SSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTAATGMTVTPASTSVVKGQSTTLT
                     VAFQPEGVTDKSFRAVSADKTKATVSVSGMTITVNGVAAGKVNIPVVSGNGEFAAVAE
                     ITVTAS"
BASE COUNT          270 a          394 c          323 g          281 t
ORIGIN      
        1 agtagggata actccattcc cgtccgggcc aaagcggaca cctccggcaa gccctgccgc
       61 tttctgcatc agcacatcat cttcaggctc ttcgtcagcc tcgcgccggt tcagcagact
      121 gaaatccagc ggatgcatat ccggatcgct gaaaaacagg ctgagcacgg tgtacgtcag
      181 cccggaaaag tgcatatcca gcagaacatc atgaaaataa tgggtactgt aaaagcggtg
      241 ccagtcggca tactccgtgg atgacatccc ggcaagcatg gcacgccagt cgggtcgccc
      301 catctcacgc gccagtttca gggcaaaact cagctcaccg tcgaacactt tcccgcagaa
      361 acaggctctg cgggcccggc gtcctctgtc tgttcagggg cattattcac aacaaactca
      421 tacataccag acagccggta caccacgttt tcagcatgag aaattgcctc cgtgggccag
      481 gtggtaagca cttcctgctc aatctgttta acggcttcat tcatggacgg catctgcgtc
      541 ttctgcggat ggttatgcca cagggacatc gccaccagaa acgcgccggt tctgatggcg
      601 tcttccacag taaacttccg gttgctgtct gactccgcct gttctgcctg ccgtttcatc
      661 agggcgagat gctcaatacg ctgcagggct gacagttcag aaagcgtgac ggtcacaccg
      721 ttatgttcaa atgattcggt tttcaggaac atctctgact ctccggatta actggcggtg
      781 acggtaattt ctgcaaccgc agcaaactca ccattaccgg atacaaccgg aatgttgacc
      841 ttgcctgcag caacgccgtt cacggtgatg gtcataccac tgaccgacac ggtggctttt
      901 gttttatccg cagacaccgc acgaaagctc ttgtcggtta cgccctccgg ctggaaggcc
      961 acggtcagcg tggtgctctg ccctttcacc accgaggtgc tggcaggcgt cacggtcatg
     1021 ccggttgccg ctgtgaccgt gctgcgatct tctgccatcg acggacgtcc cacattggtg
     1081 actttcaccg tgcgggtgat cacttccttc gccgtcaccg ccttaccgat actactgacc
     1141 cagccacgga acacatcgac cgtgccgttc gggaagcgga ttttataggc acgggtatcg
     1201 ccttcattaa accacgccag cagcgcctgc tgcccctgct ctccgggcat ccacgccagc
     1261 gtgaagct
//
LOCUS       DAJTZL010000377         1264 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.380, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000377 DAJTZL010000000
VERSION     DAJTZL010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1264
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.380"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(23..>1264)
                     /locus_tag="OO544_004367"
     CDS             complement(23..>1264)
                     /locus_tag="OO544_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR6769783.1"
                     /translation="IKHIIIAERAHQESLVKQKRIAQVWNHKTQLARELKKPMGKQAP
                     GWLELSEDGSYYIVDEDKASLVNIIYDKRLSGMSMFAICKWLNEQGYLTINQRKVRIS
                     KTKKPDGNWSALSVKHILTSRSVLGYLPAKISTEDRKTVLREEIEGFYPQIVTDSKFY
                     AVQQLLEETGKGKTSSGEHWLYVNILKGLIRCKCGLVMTPTGIRKPVYQGTYRCNGNK
                     ESRCSYGTVSRKLLDTQLCSRLFSKLSQLHDEATDTAKLDELQRRLNTVDGELEKLTE
                     TLIQLPNITQIQEALRVKQEEKDELIVQLSREKARVKSVSSLDLSGLDMESVEGRTEA
                     QIIIKRLVKEIVVSGNEKLVDIYLHNGNMIRGFPLDGKDDHTLTLEEATDEMQSLDDM
                     LIFGEPVTRIYPAGDMEEVDA"
BASE COUNT          334 a          298 c          227 g          405 t
ORIGIN      
        1 cagtgaaccc taacaaaaaa tactaagcgt ccacctcttc catatcacca gcaggataaa
       61 tccttgttac tggttcacca aaaataagca tatcatctaa tgactgcatc tcatcggttg
      121 cttcttcaag cgttaaggtg tggtcatctt taccatcaag aggaaaacca cgaatcatat
      181 ttccattgtg aagatatata tccaccagct tttcattacc agaaacaaca atctctttca
      241 ccagacgctt aataataatc tgggcttcag ttctcccctc tacagactcc atatcaagac
      301 cgcttaaatc caagcttgat acagatttta ccctcgcctt ttctcgtgat aactgaacta
      361 tcagttcatc tttttcttcc tgtttaaccc gtagtgcttc ttgaatctga gtgatgttag
      421 gaagctgtat cagcgtctca gtgagttttt ccagttcacc atcaacggtg tttaatcttc
      481 tttgtagttc atctaattta gctgtatcgg tagcttcatc gtgtagctgt gacagtttgc
      541 taaacagacg actacaaagc tgtgtatcaa gtaactttct ggacactgta ccgtagctac
      601 aacgggactc cttattacca ttgcaacggt aagtaccttg ataaacgggt ttacgtatcc
      661 ctgtaggagt cattacaagc ccacacttac aacggataag ccccttcagt atgttcacat
      721 atagccagtg ttcaccgctg gatgttttcc ccttccctgt ttcctctaac agttgctgta
      781 cagcgtaaaa cttagagtct gtgacaatct gaggatagaa cccttcaatt tcttcacgta
      841 acactgtttt acggtcttct gtagaaatct tagcgggaag gtaacctaat acacttcgtg
      901 aagtcaggat gtgtttaacg cttaatgctg accagttgcc atccggtttc ttagtcttgg
      961 atattctgac cttacgctga ttaatggtca ggtaaccttg ttcgtttagc catttacaga
     1021 tagcaaacat cgacatacca gaaagacgct tgtcatagat gatattcacc agtgacgctt
     1081 tatcttcatc aacgatgtag tatgaaccat cttctgatag ctctaaccat ccgggggctt
     1141 gcttacccat aggcttctta agttctcttg caagctgtgt tttatggttc cagacctgag
     1201 ctatacgctt ttgcttaaca agagattctt ggtgtgctct ctcagctatg atgatgtgct
     1261 taat
//
LOCUS       DAJTZL010000378         1262 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.381, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000378 DAJTZL010000000
VERSION     DAJTZL010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1262
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.381"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            62..563
                     /gene="tnpA"
                     /locus_tag="OO544_004368"
                     /pseudo
     CDS             62..563
                     /gene="tnpA"
                     /locus_tag="OO544_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(581..1249)
                     /locus_tag="OO544_004369"
                     /pseudo
     CDS             complement(581..1249)
                     /locus_tag="OO544_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
BASE COUNT          331 a          289 c          282 g          360 t
ORIGIN      
        1 ctcctgttga ccacctggtc ttaactggaa tttaaatgcc tgtaatcgtt tcacaggtca
       61 aatgatactt tggtctgtga aaaaagaaac cgatatccgg cgtggaaggc attgtgtttt
      121 cctgatgcat gttcacctgg tctttgtcac tagataccga cgccagattt tttgacgcga
      181 cagaaaaact acgcacttac ttttcaaatg tatgtgctta ttttgaagct gaactggttg
      241 aaatggatgg cgaaccagat cacgtccatt tgttaattaa ctaacctccc aaactggcga
      301 tatccagtct ggtaaacagc ctcaaaggcg tatcgggtag gttactgcga cgagatcgac
      361 cagatattgc agtcaggtat tactacaaag gcgttttgtg gagtcctggc tattttgcca
      421 gtagctgcgg aggtgtgcca atatccgtca tccgccaata cattgaacaa cagcaaacac
      481 ctggtcaggt ggaaaaccgc gccttatatc cccgccctga aggatggggt tttacggcgc
      541 accggataaa aagcgccatc taacgatggc gcgtctggaa ctactttttg ttaacgtcaa
      601 acatggtggc atcggtcgcc atgtcatcaa taacttgttt aatattttcg aaagccattg
      661 gcgtactttc gttattcagg tctttacctt cgccctgacg aacgactttg ataaccggtt
      721 tattagtcgc tgcatcaatc agctcacctt caaaatagag tcgagtatcc atggtacggt
      781 ggcctgtagc catttgcgtc cccgcaacca ctaatgcaac aggaaccact tcatagaatt
      841 gcagcccttc tttgctggta tctacaccgg taatggcccc acggaaaatc agactacgcg
      901 gcccagcggt ggtaaccagt ggtttacgct gcgctatcgc ttctttcatt tcggtgttgg
      961 tatagttcaa aattttatcc agaactttct gccctaccta ggtagaaggt tttggcaccg
     1021 gataataagt gattgggttc cagacgatgc tgtcatattt gctttgatca aaactcgggt
     1081 ctacccaacg taaaacaggt ttacctgttg ctgaggttgt ttcttttaaa tcagagtaat
     1141 tgtttaaaaa accagaatat ttatctggct gggtgatttt agaggcacaa ccagacagag
     1201 ccaacaagcc gcataaagct gcaactttcg caaatgatgt ggtacgcatg aatagtttcc
     1261 tg
//
LOCUS       DAJTZL010000379         1260 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.382, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000379 DAJTZL010000000
VERSION     DAJTZL010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1260
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.382"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..164)
                     /locus_tag="OO544_004370"
     CDS             complement(<1..164)
                     /locus_tag="OO544_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415986.1"
                     /note="involved in the transport of nitrate and nitrite;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HCR6769784.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSA"
     gene            complement(487..1260)
                     /locus_tag="OO544_004371"
                     /pseudo
     CDS             complement(487..1260)
                     /locus_tag="OO544_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310100.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
BASE COUNT          408 a          189 c          250 g          413 t
ORIGIN      
        1 gcgctaaata gcatccagac acagaaggca agaagtagac aactgactga tatccagagg
       61 tttcttcgag caatatgttt tcctttattt tcccagaagg ccggattttc tggtttccag
      121 tcgcgtaaaa gataacgact atttttctca ttttgcagtg ccatattgtt cctcacatgc
      181 acacattggt aatgaaaaaa gacaaaacag gaggtaaggc gcaatagcca gttattagaa
      241 ttaaggatga attgggtgaa gtgctgattg aaagaataga taagaaagcg taaccgcggg
      301 ggcagaatgg ggattaagta gccagatatg tgttactaaa tgtaactaac aagctaacct
      361 tcaaacgggg ttaatctttg aacgtatgtc atgtttcaac ccttcagatc gtgaatctaa
      421 agggttacat attaactata ttatcaacaa gaccagacac ttatagaatt agtgatgatt
      481 tattcactaa aaaggccatg aactggagga aaacgatctg tccagaaggt ctatcggttc
      541 ctctcttatt gcctgattgt aataatcgtt attacagtaa gtattgttta tttcatctaa
      601 ggtcatgttt ttaactttta tattcctctg gtttacaact tcctctgtct tgtaagtgtg
      661 tatattatat ggggtatatg ctatttttaa taggatagtg aaacaattcg ttatccacat
      721 ctattccgtc agagtgaact tcaaatgcac caggcgaaag aattgccagg ccagtattaa
      781 gcttaagtgt tggggaaaac aaattaaata acatgagctg catagtaggg caattgttct
      841 tcccagaaac atttaaaaaa tttaagtttt tgctctgttt tattatggga aataaagttc
      901 caatatcgga tgtattatta gtatcaatgt gggttatttt attgttgtcc agtcttaaac
      961 ttttaataga tggaagacaa gtcaggaaat caagatttat atttttcaac tggttgttag
     1021 cagcgtttaa tgtctgaaca ttgcagaagt tgctatagaa tattttaatt tcagaaagca
     1081 ggtttttgtt aataagtaac gtattcaagt tgggaaataa gacattgtgt aaacttgtta
     1141 gtttattatg ggatagatcc agcagtaaga gttaattttt attacctgca acaatatcag
     1201 ataattgatt atggctgaga ttcaggtatt gcagacattc gcaagattct aattgcacaa
//
LOCUS       DAJTZL010000380         1252 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.383, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000380 DAJTZL010000000
VERSION     DAJTZL010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1252
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.383"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            386..619
                     /locus_tag="OO544_004372"
     CDS             386..619
                     /locus_tag="OO544_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HCR6769785.1"
                     /translation="MSTKNRTRRTTTRNIRFPNQMIEQINIALVQKGSGNFSAWVIEA
                     CRRRLCSEKRVSPEANKEKSDITELLRKQVRPD"
     gene            677..1087
                     /locus_tag="OO544_004373"
     CDS             677..1087
                     /locus_tag="OO544_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HCR6769786.1"
                     /translation="MAQVAIFKQIFDKVRNNLNYHWFYSELKRHNVSHYIYYLATENI
                     HLVLENDNTVLIKGQGKVVNVRFSKNKCLIEATLKGFKSGELSFYEYRKNLATAGVFR
                     WITNIHENKRYYYTFDNSLLFTENIQNTTQIFPH"
BASE COUNT          431 a          279 c          234 g          308 t
ORIGIN      
        1 tcattccacg gacaaaaaca gagaaaggaa acgacagagg ccaaaaagcc cgttttcagc
       61 acctgtcgtt tcctttcttt tcagggggtg ttttaaataa aaacattagg ttacggcgaa
      121 gaagaacgga aacgccttaa accggaaaat tgtcataaat agcgaaaacc cgcgcgcctt
      181 ccgccccgta acctgtcgga tcgccggaaa ggacccgcaa aatgataata attatcatct
      241 acatgtcaca acgtgcatct acgccatcaa accacgtcaa ataatcaatt atgacgcagg
      301 tatcgtatta attgatctgc atcaacttaa cgtaaaaaca acttcagaca atacaaatca
      361 gcgacactga atacagggca acctcatgtc aacgaagaac agaacccgca gaacaacaac
      421 ccgcaacatc cgctttccta accaaatgat tgaacaaatt aacatcgctc ttgttcaaaa
      481 agggtccggg aatttctcag cctgggtcat tgaagcctgc cgccggagac tgtgctcaga
      541 aaaaagagtt tcgcctgaag caaacaaaga aaagagtgac attactgaat tgctcagaaa
      601 acaggttaga ccagattgaa gcaatttaga taatcgtgca gactacgccc cctcatatca
      661 catggaaggt actacaatgg ctcaggttgc catttttaaa caaatattcg ataaagtgcg
      721 aaataattta aactatcact ggttttattc tgaactaaaa cgtcacaatg tctcacatta
      781 catttactat ttagccacag agaatattca tcttgttctt gaaaacgata atacggtttt
      841 aataaaagga cagggtaagg ttgtaaatgt aagattttca aaaaataaat gccttataga
      901 agccacctta aaaggattca aatcaggaga gttatcattt tacgaataca ggaaaaatct
      961 tgctacagca ggggttttca gatggattac aaatatccac gaaaacaaaa ggtattacta
     1021 tacctttgat aattcattac tctttactga gaacatacag aacactacac aaatatttcc
     1081 gcactaaatc ataacgtccg gtttcttccg tgccagaacc ggactcgctg gcatgatgaa
     1141 atatgtgtac ccggtaaccc cggtgtgcat cgtttttgat tattcccgca cactcgcgca
     1201 gaaggagttc cccgtcgggc tacggtctct gttaatacgg gaatacggcg ac
//
LOCUS       DAJTZL010000381         1241 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.384, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000381 DAJTZL010000000
VERSION     DAJTZL010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1241
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.384"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>1241
                     /locus_tag="OO544_004374"
     CDS             <1..>1241
                     /locus_tag="OO544_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR6769787.1"
                     /translation="DRMLVLSRAGQAAGLTFNQTSESLSALVKAGVSGEAQIASISQS
                     VARFSSASGVEVDKVAEAFGKLTTDPTSGLTAMARQFHNVTAEQIAYVAQLQRSGDEA
                     GALQAANEAATKGFDDQTRRLKENMGTLETWADRTARAFKSMWDAVLDIGRPDTAQEM
                     LIKAEAAFKKADDIWSLRKDDYFVNDEARARYWDDREKARLALEAARKKAEQQTQQDK
                     NAQQQSDTEASRLKYTEEAQKAYERLQTPLEKYTARQEELNKALKDGKILQADYNTLM
                     AAAKKDYEATLKKPKQSGVKVSAGDRQEDSAHAALLTLQAELRTLEKHAGANEKISQQ
                     RRDLWKAESQFAVLEETAQRRQLSAQEKSLLAHKDETLEYKRQLAALGDKVTYQERLN
                     ALAQQADKFAQQQRAKRAAIDA"
BASE COUNT          321 a          306 c          414 g          200 t
ORIGIN      
        1 gatcgtatgc tggtcctgtc cagagccggg caggcggcag ggctgacgtt taaccagacc
       61 agcgagtcac tcagcgcact ggttaaggct ggggtaagcg gtgaggctca gattgcatcc
      121 atcagccaga gtgtggcgcg tttctcctct gcatccgggg tggaggtgga caaggtcgct
      181 gaagccttcg ggaagctgac cacagacccg acgtcagggc tgacagcgat ggcacgccag
      241 ttccataacg tgacggcgga gcagattgcg tatgttgctc agttgcagcg ttccggcgat
      301 gaagccgggg cattgcaggc ggcgaacgag gccgcgacga aagggtttga tgaccagacc
      361 cgccgcctga aagagaacat gggtacgctg gagacctggg cagacaggac agcgcgggca
      421 ttcaaatcca tgtgggatgc ggtgctggat attggtcgtc ctgataccgc gcaggagatg
      481 ctgattaagg cagaggctgc gtttaagaaa gcggacgaca tctggagtct gcgcaaggat
      541 gattattttg ttaacgatga agcgcgggcg cgttactggg atgatcgtga aaaggcccgt
      601 cttgcgcttg aagccgccag aaagaaggct gaacagcaga ctcaacagga caaaaatgcg
      661 cagcagcaga gcgataccga agcgtcacgg ctgaaatata ccgaagaggc gcagaaggct
      721 tacgaacgcc tgcagacgcc gctggagaaa tataccgccc gtcaggaaga actgaacaag
      781 gcactgaaag acgggaaaat cctgcaggcg gattacaaca cgctgatggc ggcggcgaaa
      841 aaggattatg aagcgacgct gaaaaagccg aaacagtccg gcgtgaaggt gtctgcgggc
      901 gatcgtcagg aggacagtgc tcatgctgcc ctgctgacgc ttcaggcaga actccggacg
      961 ctggagaagc atgccggagc gaatgagaaa atcagccagc agcgccggga tttgtggaag
     1021 gcagaaagtc agttcgcggt actggaggag acggcacaac gtcgccagct gtccgcacag
     1081 gagaaatccc tgctggcgca taaagatgag acgctggagt acaaacgcca gctggctgca
     1141 cttggcgaca aggttacgta tcaggagcgc ctgaacgcgc tggcgcagca ggcggataaa
     1201 ttcgcacagc agcaacgggc aaaacgggcc gccattgatg c
//
LOCUS       DAJTZL010000382         1233 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.385, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000382 DAJTZL010000000
VERSION     DAJTZL010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1233
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.385"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(1..761)
                     /locus_tag="OO544_004375"
                     /pseudo
     CDS             complement(1..761)
                     /locus_tag="OO544_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935599.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
BASE COUNT          308 a          299 c          308 g          318 t
ORIGIN      
        1 aaagtctcca tggtacagac ccgtgcgcca tcaggaacgg tttcggtgaa tctgatgttg
       61 ccgcgttcgt cgaagtgaac aaccagggtg cggccatcgc ctggaattat tttgtcagtg
      121 ggtggggtgt tattctggcg cagttcggcc tccatgcgat cgaactcagc aatgtaggct
      181 tctttaaatg cagcggcttt tttgccagtg aatcccatca ccaggaaaac gaagccgttt
      241 ttggtgattt ggtacatcgg gcgtttttcg cctttggcgt cagtgtaggt gacgtcctca
      301 aaattgaggg cgttaaattc aggtgagcat tcagtatttg cgatggcacg taacacattg
      361 tcgtgacgct tgccaaagaa ctctgcgatc gcaatagacg tagtgacagc gcgaccattt
      421 tcgatggtta cgtcagggtg agaaagggca gggatagtag ccatgatggc atcctcgagt
      481 gataagttaa ttaactcacc accgaggttt tccacgacca taagggtggt gagacgtaca
      541 ggggtggaaa taccggtcac tcgagaaccc ggccagtctt gcgactgccc tgcacatccc
      601 accataattt gaatggagct gtgcattacg cataaaaaaa ccgcttcagc gcggctatgc
      661 gctcaagtaa cgttcgggtt tccacgcccg gcacccgttt tataaggtgc aggtgcacta
      721 taattccacc cgttctggtt ttcaatagct acattcaaca ttttctcttt cctttcatca
      781 ccgaagtgaa ctttgttgat gcggtgcctg gtgcctccag gtgacgttaa ccagttaaca
      841 attaacgccg gatacaggga aacccataac cacccgatac gggggaacta atcgcttttt
      901 aactgtgccg cgtgcgctga gccgcattca ccgcatcaca aaattcactt taaaaagggc
      961 ggacatcagc cagcaatgaa actgatgccg ccaaaggtac caatcaacat ggagtgttgt
     1021 ggcgtggttg tcacttaagc gtatggtcaa cctgacaacc cggtgtcctc aacggggaag
     1081 gaataactcc gccatactta ccgccgcgtc atttcgcgtt gtgtgcctgc ttttaaccac
     1141 gtcaggcgag gtggtatcct tcttatcccg aataaccaag aaggaaatct atatgactaa
     1201 agaagaattt gtctcttata tttttgataa aac
//
LOCUS       DAJTZL010000383         1211 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.386, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000383 DAJTZL010000000
VERSION     DAJTZL010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1211
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.386"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..680)
                     /locus_tag="OO544_004376"
     CDS             complement(<1..680)
                     /locus_tag="OO544_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR6769788.1"
                     /translation="MLPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGE
                     NRETAFTRLQLCLENSETSLDLSCLGLRSLPRLPDNLDEINVSNNQLSMLPELPRALK
                     ELNASSNQLSALPELPVSLEYINVSDNHLFALPELPASLEYINVSDNHLSVLPRLPMS
                     LELLDAARNALEVIPDFPERDDHIIRIFWLNQNRITAIPESILGLSSDSVVNLRENQL
                     SPRIMQTL"
     gene            complement(996..>1211)
                     /locus_tag="OO544_004377"
     CDS             complement(996..>1211)
                     /locus_tag="OO544_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HCR6769789.1"
                     /translation="DGTLYGATILGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLT
                     HILSEHFENVKTKVQGTVVMFSASGKK"
BASE COUNT          355 a          211 c          252 g          393 t
ORIGIN      
        1 aacaaagttt gcattattct gggagatagt tgattttctc taagattgac aacgctatca
       61 gaactgaggc caagtatgct ttccggaatt gccgtgatcc ggttctgatt aagccagaat
      121 attcttataa tatgatcatc tctttctgga aaatctggta ttacttccaa agcatttctg
      181 gctgcatcaa gtaattccaa tgacattggt aacctcggaa gtacagacag gtgattgtca
      241 cttacattaa tatattctag tgacgcaggt aattcaggaa gtgcgaacaa atggttatca
      301 ctcacattta tatattccag cgacactggt aattcaggaa gtgcagataa ttgattactg
      361 cttgcattca gctctttcaa tgcccttggt agctcgggga gcattgatag ttggttattg
      421 cttacattaa tttcatcaag attgtcaggc aatcgtggta gagatctgag acctaaacaa
      481 gataagtcca acgatgtttc actgttctcc agacataatt ggagccgggt aaaagcagtt
      541 tccctgtttt ctccggaaat gctgttttta gtccattcaa cccattcgga gagataatta
      601 ttgtgaacat tgtcgattga tgtagttctg taaaaagaga cgtttccagt ggataggggg
      661 ggattattta caggaagcat aataacctcg cagaaggata ttctgataaa atgtgatact
      721 gggaaataat aagctgaaat aaaatgtttc aggattgaaa tttattgttt tcattttaag
      781 gaagagatgt tgatttaact gtaaaaaaac gatactattt tcttagatgg taaatgtctc
      841 gcctgtgcta tattttgttt ttcggttagt taaatatcgt acgttcatat ttgaacgttc
      901 tgccggaatg cattatcaat agaggtaaag tcgcaacccc aaatcgtaaa ggaaaccgta
      961 gcacgtctta agcaagaacg tgctgcggtt ggatgctatt ttttccctga agcggaaaac
     1021 attactacag taccttgaac cttggtttta acattctcga aatgctctga gagtatatgt
     1081 gttaagcctt cttcggaatc ttttgtgttt gaaaagatgc ctttctgatt gtaaatgcgc
     1141 atcagttttt gaccgaagct attgtgcaca actccatcgc caagaattgt ggctccgtat
     1201 agagttccat c
//
LOCUS       DAJTZL010000384         1211 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.387, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000384 DAJTZL010000000
VERSION     DAJTZL010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1211
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.387"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            229..>1211
                     /gene="gspA"
                     /locus_tag="OO544_004378"
     CDS             229..>1211
                     /gene="gspA"
                     /locus_tag="OO544_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspA"
                     /protein_id="HCR6769790.1"
                     /translation="MSTRREVILSWLREKRQTWRLCYLLGEAGSGKTWLAQQLQKDKH
                     RRVITLSLVVSWQGKAAWIVTDDNAAEQGCRDSAWTRDEMAGQLLHALHRTDSRCPLI
                     IIENAHLNHRRILDDLQRAISLIPDGQFLLIGRPDRKVERDFKKQDIELVSTGRLTEH
                     ELKASILEGQNIDQPDLLLTSRVLKRIALLCRGDRRKLALAGETIRLLQQAEQTSVFT
                     AKQWRMIYRILGDNRPRKMQLAVVMSGTIIAITCGWLLLSSFTAPFPVPAWLVPVTPV
                     VKQDMTKDIAHVVMRDSEALSVLYGVWGYEVPADSAWCDQAVRAGLACKSG"
BASE COUNT          328 a          249 c          309 g          325 t
ORIGIN      
        1 catctttatt atgattcgcc agatggaagg ggaaacgtag tgtgggcacg atgtatgttc
       61 taataaaata gattgggtgg gttgtatatt gacgttagta taaatagtat taatgccgct
      121 tgcatgaatc aagcaaaatt tatttatatt agtaatatgt atttaattaa taaatacata
      181 cattattttt atgattaagg ataatcaata attaaaacag gaagttctat gtctacgaga
      241 agagaagtta ttctttcctg gttgcgtgag aaacgtcaga cctggcgtct gtgctatttg
      301 ttgggtgagg ctggaagtgg aaaaacctgg ctggcgcagc aactgcaaaa agataaacat
      361 cgccgtgtga ttactttaag cctcgttgtt tcctggcaag gtaaggccgc atggatcgtt
      421 accgacgata acgcggctga acagggctgc cgtgacagcg cctggacgcg agatgagatg
      481 gcggggcaat tactgcatgc gcttcatcga actgatagtc gctgccctct tattattatt
      541 gaaaatgctc acctgaatca ccgccgcata cttgatgatc tccaacgcgc gatatctctt
      601 attcctgacg gtcaattttt gctgatcggc agacccgatc gaaaagtcga acgtgatttt
      661 aaaaaacagg acattgaact tgtctcgaca ggacgtctga cggagcacga acttaaggca
      721 agcatccttg aaggtcagaa tattgaccaa cccgatctcc ttttaacctc cagagttctg
      781 aaacggatag ctttattatg tcggggcgat cgcagaaagc tggcgcttgc cggcgaaaca
      841 attcgcttat tacagcaagc cgagcagacc agcgttttca ctgcaaaaca atggcgaatg
      901 atttaccgca tattgggtga taaccgcccc cggaaaatgc agcttgccgt ggtaatgagt
      961 ggaaccatta tcgctattac ctgtggttgg ctactgctgt cctctttcac agctccgttc
     1021 cccgttcctg cgtggctggt acccgtaacg ccagtagtta aacaagatat gactaaggat
     1081 attgctcatg tggtgatgcg cgatagtgag gcgctaagcg tgttatacgg cgtatggggg
     1141 tacgaggtcc cagctgattc ggcctggtgc gatcaggcag taagagcggg gctggcatgt
     1201 aaaagtggga a
//
LOCUS       DAJTZL010000385         1207 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.388, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000385 DAJTZL010000000
VERSION     DAJTZL010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1207
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.388"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            52..>1207
                     /locus_tag="OO544_004379"
     CDS             join(52..301,301..>1207)
                     /locus_tag="OO544_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR6769791.1"
                     /translation="MKKRNFSAEFKRESAQLVVDQKYTVADAAKAMDVGLSTMTRWVK
                     QLRDERQGKTPKASPITPEQIEIRKLRKKLQRIEMENEIFKKGYRALDVRLPEQFSII
                     GKLRAHYPVVTLCHVFGVHRSSYRYWKNRPEKPDGRRAVLRSQVLELHGISHGSAGAR
                     SIATMATRRGYQMGRWLAGRLMKELGLVSCQQPTHRYKRGGHEHVAIPNYLERQFAVT
                     EPNQVWCGDVTYIWTGKRWAYLAVVLDLFARKPVGCAMSFSPDSRLTMKALEMAWETR
                     GKPGGVMFHSDQGSHYTSRQFRQLLWRYQIRQSMSRRGNCWDNSPMERFFRSLKNEWM
                     PVVGYVSFSEAAHAITDYIVGYYSALRPHEYNGGLPPNESENRYWKNSNSVA"
BASE COUNT          343 a          294 c          332 g          238 t
ORIGIN      
        1 gccacctgaa cagaggtgat atgctcacct cagaacaaca caggtgctcc aatgaaaaaa
       61 agaaatttta gcgcagagtt taaacgcgaa tccgctcaac tggttgttga ccagaaatac
      121 acggtggcag atgccgccaa agctatggat gttggccttt ccacaatgac aagatgggtc
      181 aaacaactgc gtgatgagcg tcagggcaaa acaccaaaag cctctccgat aacaccagaa
      241 caaatcgaaa tacgtaagct gaggaaaaag ctacaacgca ttgaaatgga gaatgaaata
      301 ttaaaaaagg ctaccgcgct cttgatgtca gactccctga acagttctcg ataatcggga
      361 aactcagagc gcattatcct gtggtcacac tctgccatgt gttcggggtt catcgcagca
      421 gctacagata ctggaaaaac cgtcctgaaa aaccagacgg cagacgggct gtattacgca
      481 gtcaggtact tgagctacat ggcatcagcc acggttcggc cggagcaaga agcatcgcca
      541 caatggcaac ccggagaggc taccagatgg gacgctggct tgctggcagg ctcatgaaag
      601 agctggggct ggtcagctgt cagcagccga ctcaccggta taaacgtggt ggtcatgaac
      661 atgttgctat ccctaactac cttgaaaggc agttcgccgt gaccgagcca aatcaggtgt
      721 ggtgcggtga tgtgacctat atctggacgg gtaagcgctg ggcgtacctc gccgttgttc
      781 tcgacctgtt cgcaagaaaa ccagtgggct gtgccatgtc gttctcgccg gacagcaggc
      841 tcaccatgaa agcgctggaa atggcatggg aaacccgtgg taagcccggc ggggtgatgt
      901 tccacagcga tcagggcagt cattatacga gcaggcagtt ccggcagtta ttgtggcgat
      961 accagatcag acagagtatg agccggcgcg gaaactgctg ggataacagc ccaatggaac
     1021 gcttcttcag gagtctgaag aacgaatgga tgccggtggt gggttacgta agcttcagcg
     1081 aggcagctca cgccataacg gactatatcg ttggatatta cagcgcacta agaccgcacg
     1141 aatataacgg tgggttaccc ccaaacgaat cggaaaatcg atactggaaa aactctaact
     1201 cggtggc
//
LOCUS       DAJTZL010000386         1198 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.389, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000386 DAJTZL010000000
VERSION     DAJTZL010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1198
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.389"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            223..1038
                     /locus_tag="OO544_004380"
     CDS             223..1038
                     /locus_tag="OO544_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6769792.1"
                     /translation="MISLKAPHNNLMPYTQQSILNTVKNNQLPEDIKSSLVSCVDIFK
                     VLIKQYYDYPYDCRDDLVDDDKLIHLMAAVRDCEWSDDNALTINVQFNDFPGFYDWMD
                     YPDHPVKFVFRILENQKGTVWVYDQDDAFLDIKANVQAGRFTGLKKLVQFINSVRTDC
                     KCILLEHHMPLLRLFPKGKECMHVEKWLREMSSIPETDAPIKQALAHGLLLHLKNIYP
                     VFPESLVMLLLSVLDVKTYRDDARLNEWISNRVQELGDRYYPVNKHVKIRYTL"
BASE COUNT          364 a          230 c          233 g          371 t
ORIGIN      
        1 tggtttaaca tattaacttc gctctccact taacttttta gttaaggaga gcgaaataat
       61 atcaaagtag cagtaaaacc tataacgtaa attcaaattg ttaaattaac tccctccagt
      121 acacaatact tctcacgtta gttatgagcg atttctgata gtgcctggtt taatcagcgc
      181 tttattatct gcgacgttta tttttattta agagagatcg cgatgatatc attaaaagct
      241 ccgcacaata atttgatgcc atatacgcaa caaagcatac ttaatacggt taaaaacaat
      301 cagttaccag aggatatcaa aagctcccta gtttcctgtg tcgatatatt caaggttttg
      361 attaaacaat attatgatta tccttatgat tgtcgtgatg atttagtcga tgacgacaaa
      421 ctcattcatc tcatggctgc cgtacgagac tgtgagtggt cagacgataa cgcactcacc
      481 ataaatgtgc agtttaatga tttcccagga ttttatgact ggatggatta ccctgatcac
      541 ccggttaagt ttgtttttcg catacttgaa aatcaaaaag ggacagtttg ggtatatgac
      601 caggatgatg cgtttcttga tattaaagca aatgtgcagg ctggacgctt taccgggctt
      661 aagaaattag tgcagtttat caattcagtg cgaacagatt gcaaatgtat tcttcttgaa
      721 caccatatgc ctttgctgag acttttccct aaagggaaag agtgtatgca tgtggaaaaa
      781 tggcttcgcg aaatgtcgtc tattccggag acagacgcgc ccattaagca ggcgcttgcg
      841 catggattat tattacactt aaaaaatatc taccctgttt ttcctgaatc actggtgatg
      901 ttattgctct ctgttctgga cgtaaaaaca tacagagatg atgcacgatt aaatgaatgg
      961 atatctaata gagtgcagga attaggtgac agatattatc ctgtaaataa gcacgttaaa
     1021 atccgctaca ctctttagtt cagaaagggt tccacctgca agttgttatt ccaccagaga
     1081 gtgaatcgca aagcaggtaa tcatttcatc tgtatatccg tatgccaaat ggcattccac
     1141 cgccttttcg ccgatatttt gttgtgatcg gcgacacttc gcttaaaaaa gcaccagc
//
LOCUS       DAJTZL010000387         1189 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.390, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000387 DAJTZL010000000
VERSION     DAJTZL010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1189
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.390"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..294)
                     /locus_tag="OO544_004381"
     CDS             complement(<1..294)
                     /locus_tag="OO544_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001572122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCR6769793.1"
                     /translation="MSRKSCLIRLFKTTVSCKLFTAIILSVFLGQPALTYAGVVIGGT
                     RVVYLSNNADKSISVFSKEEKIPYLIQAWVDPFNKEDKSKAPFTVIPPVSRLEP"
     gene            complement(342..914)
                     /locus_tag="OO544_004382"
     CDS             complement(342..914)
                     /locus_tag="OO544_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA-O113"
                     /protein_id="HCR6769794.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVHFVGEIVDTTCEVTSD
                     TADQIVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTEAPGGDGDL
                     KVGTPLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSQVKLYNDSAYTAIDAEGKAEM
                     KFIARYVATNATVTAGTANADSQFTVEYKK"
BASE COUNT          379 a          246 c          220 g          344 t
ORIGIN      
        1 aggttctaat cgtgatactg gcggaataac ggtaaacggc gctttgcttt tatcctcttt
       61 attaaatgga tctacccaag cctggatgag ataaggtatt ttttcttctt tgctaaatac
      121 agaaatagat ttatcagcat tattacttaa gtaaaccacc cgcgtaccac caatgacaac
      181 gcctgcataa gttaatgctg gttggcctaa aaaaacgctg agtataatcg cggtaaataa
      241 cttacatgaa actgttgttt taaagagcct gattaaacaa ctttttctgg acataacgac
      301 tccctggcca aagccaactg aataaaaagg cccttgataa attatttctt atattcgaca
      361 gtaaattgtg aatcagcgtt cgccgtacca gccgttacgg tcgcattggt tgccacatag
      421 cgtgcaataa acttcatttc agccttgcct tcagcatcga tagcggtata agcagagtcg
      481 ttataaagtt taacctgcga attatcggac tggttaaaaa tacgaatacc aacgccggtt
      541 gccgcaatcg cttgtcctgt accggtaaaa tcaccagggt tgcctgctgt aagcggcgta
      601 cccactttca ggtcgccgtc gccgccaggc gcttctgtac catcaaaacg aacggctgct
      661 tgagtgtacg ttgccgggca attttcaagt ttaatactga acttctgtgg cgacgccaga
      721 ctacctacac ctgaaaatgc atttttactg actttaccca gtgggacaat ttgatcggct
      781 gtatcggagg taacttcaca agtagtgtcg acaatttcac cgacgaagtg aacaacacca
      841 tcttctgcca gtgcatgccc ggccagcatt agagatgcca gagtgaatgc accgcctata
      901 atattacgct tcataaaaac tccattgata taatatatcc tttaccatga atcatcactt
      961 atattaataa gtgtattttt gattcaaagg aatagtacac caaagagacg gctttatcta
     1021 aaaataatat taattacgca aaaataaaat ataaattcat tgtttaaata ttatttaaac
     1081 agtaaaaaat caagtttgtt atcagacatt aatgataatt cagaatatat ttatatagaa
     1141 agatagataa atttattaat gtcagttata aaaaagggcc accggtcgg
//
LOCUS       DAJTZL010000388         1180 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.391, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000388 DAJTZL010000000
VERSION     DAJTZL010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1180
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.391"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>1180
                     /locus_tag="OO544_004383"
     CDS             <1..>1180
                     /locus_tag="OO544_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HCR6769795.1"
                     /translation="AEQEALIHALQEKLSNREREIDHLQAQLDKLRRMNFGSRSEKVS
                     RRIAQMEADLNRLQKESDTLTGRVYDPAVQRPLRQTRTRKPFPESLPRDEKRLLPAAP
                     CCPNCGGSLSYLGEDTAEQLELMRSAFRVIRTVREKHACTQCDAIVQAPAPSRPIERG
                     IAGPGLLARVLTSKYAEHTPLYRQSEIYGRQGVELSRSLLSGWVDACCRLLSPMEEAL
                     HGYVLTDGKLHVDDTPVPVLLPGNKKTKTGRLWAYVRDDRNAGSTLAPAVWFAYSPDR
                     KGIHPQTHLAGFSGVLQADAYAGFNELYRDGRITEAACWAHARRKIHDVHVRTPSALT
                     EEALKRIGELYAIEAEIRGMTAEQRLAERQLKTKPLLKSLESWLREKMKTLSRHSELA
                     K"
BASE COUNT          263 a          336 c          373 g          208 t
ORIGIN      
        1 gccgaacagg aagcgctgat ccacgccctg caggaaaagc ttagcaaccg ggagcgcgaa
       61 atagaccatc tgcaggcgca gctggataaa ctccgccgga tgaacttcgg cagtcgttcc
      121 gaaaaagtct cccgccgtat cgcacaaatg gaagccgatc tgaaccggct tcagaaagag
      181 agcgatacgc tgactggtag ggtgtatgac ccggcagtac agcgtccgtt gcgtcagacc
      241 cgcacccgta agccgttccc tgaatcacta ccccgtgacg aaaagcgact gttgcctgcg
      301 gcgccgtgct gcccgaactg cggcggttca ctgagctatc tgggcgagga taccgccgaa
      361 cagctggagt tgatgcgtag cgccttccgg gttatccgga cggtacggga aaaacatgcc
      421 tgtactcagt gcgatgccat cgtgcaggca cctgcacctt cgcggcccat cgagcggggt
      481 atcgccggac cggggctgct ggcccgcgtg ctgacctcga agtatgcaga gcacaccccg
      541 ctgtatcgcc agtcagaaat atacggccgg caaggtgtgg agctgagccg ttcactgctg
      601 tcgggctggg tggatgcatg ctgccggctg ctgtctccga tggaagaggc gcttcatggc
      661 tatgtgctga ctgacggtaa gctccatgtt gatgacacgc ctgtcccggt gctgttgcca
      721 ggcaataaga aaacgaagac cgggcggttg tgggcgtatg ttcgtgacga ccgtaacgcc
      781 gggtcaacgc tggcgccggc ggtgtggttc gcttacagcc cggacagaaa aggcatccat
      841 ccgcagaccc atcttgcggg gttcagtggt gtactgcagg cggatgcata cgccgggttc
      901 aacgagctgt accgggatgg ccggataacg gaagccgcct gttgggctca cgcccgccgt
      961 aaaatccacg atgtgcacgt tcgcaccccg tcagccctga cggaggaagc gctgaaacgg
     1021 atcggcgaac tgtacgccat cgaggcagag ataaggggaa tgacggcgga acagcgcctt
     1081 gccgaacgtc agttgaaaac gaaaccgctg ctgaaatccc tggaaagctg gctgcgtgaa
     1141 aagatgaaaa ccctgtcgcg acactcagaa ctggcgaaag
//
LOCUS       DAJTZL010000389         1180 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.392, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000389 DAJTZL010000000
VERSION     DAJTZL010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1180
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.392"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..999
                     /gene="htrE"
                     /locus_tag="OO544_004384"
     CDS             <1..999
                     /gene="htrE"
                     /locus_tag="OO544_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Yad fimbria usher protein HtrE"
                     /protein_id="HCR6769796.1"
                     /translation="WASGQNRSNYSIGYSNSASWGSYSVSAQRSWNEDGDTDDSVYLS
                     FTIPIEKLLGTEQRTSGFQSIDTQMSSDFKGNNQLNVSSSGYSDNARVSYSVNTGYTM
                     NKASKDLSYVGGYASYESPWGTLAGSVSANSDNSRQVSLSTDGGFVLHSGGLTFSNDS
                     FSDSDTLAVVQAPGAQGARINYGNSTIDRWGYGVTSALSPYHENRIALDINDLENDVE
                     LKSTSAVAVPRQGSVVFADFETVQGQSAIMNITRSDGKNIPFAADIYDEQGNVIGNVG
                     QGGQAFVRGIEQQGNISIKWLEESKPVSCLAHYQQSSEAEKIAQSIILNGIRCQIQ"
     gene            1016..1120
                     /locus_tag="OO544_004385"
                     /pseudo
     CDS             1016..1120
                     /locus_tag="OO544_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414680.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
BASE COUNT          358 a          235 c          276 g          311 t
ORIGIN      
        1 tgggcttccg gacaaaatcg tagcaattac tctattggct acagtaacag tgcatcctgg
       61 ggcagctaca gtgtcagtgc ccagcgttca tggaatgaag acggcgacac tgacgatagc
      121 gtttatctca gtttcaccat tcctattgaa aaattacttg gcactgaaca acggacttca
      181 ggtttccaga gtattgatac tcaaatgagc agtgacttta agggtaataa ccaactcaac
      241 gtcagtagca gtggctatag tgataacgct cgcgtcagtt atagcgtaaa tactggctat
      301 acgatgaata aagccagcaa agatttgagt tatgttgggg gttatgccag ctatgaatca
      361 ccgtggggaa ccctggcagg ttcagtttct gcaaatagcg ataacagtcg tcaagtgtct
      421 ctcagcaccg acggtggttt tgtattgcat agcggcggcc tgaccttcag taatgacagt
      481 tttagcgact ccgatacact ggcggtagtt caggctccag gtgctcaagg agcgcgaata
      541 aattatggca acagtactat cgatcgatgg ggttatggtg tcaccagcgc tctttctcct
      601 tatcatgaaa accgtatcgc gctggatatc aacgatcttg agaacgatgt tgaattaaaa
      661 agtaccagtg cagtagctgt accgcgtcag ggttcagtcg tctttgctga ttttgaaacc
      721 gtgcaagggc aatcagccat tatgaacatc acacgaagtg atggtaaaaa tattccattt
      781 gctgcagata tttatgatga gcaaggcaat gtcattggta atgttggaca gggtggacaa
      841 gcatttgttc gtggcattga gcagcaggga aatatcagca ttaaatggct cgaagaaagt
      901 aaacccgtaa gttgtcttgc gcattatcaa caaagttcag aagcagaaaa aatagcgcaa
      961 tctattattc tgaatggaat tcggtgtcag attcagtaac tacaaggaac gtcaaatgat
     1021 aaaaataacg ccacataaaa tagcgatact gatgggaata ttattatcac catcagtatt
     1081 tgcaacggat attaatgtag agtttacagc cactgtcaaa gtagactggc cccctgaatc
     1141 tccagacaac cagtatcact taaataagtg atagtcttaa
//
LOCUS       DAJTZL010000390         1160 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.393, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000390 DAJTZL010000000
VERSION     DAJTZL010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1160
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.393"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            247..492
                     /locus_tag="OO544_004386"
     CDS             247..492
                     /locus_tag="OO544_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6769797.1"
                     /translation="MAVQLFKTLLNQIPLLSSLQSGTLPLFGYSGRVRPMKKAHTGES
                     GLKWEAKDSSKLIGNKDHALRGLSSPIVGIRQIRLIT"
     gene            complement(509..1141)
                     /locus_tag="OO544_004387"
     CDS             complement(509..1141)
                     /locus_tag="OO544_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308409.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HCR6769798.1"
                     /translation="MDPLHAVYLTVGLFVITFFNPGANLFVVVQTSLASGRRAGVLTG
                     LGVALGDAFYSGLGLFGLATLITQCEEIFSLIRIVGGAYLLWFAWCSMRRQSTPQMST
                     LQQPISASWYVFFRRGLITDLSNPQTVLFFISIFSVTLNAETPTWARLMAWAGIVLAS
                     VIWRVFLSQAFSLPAVRRAYGRMQRVASRVIGAIIGVFALRLIYEGVTQR"
BASE COUNT          351 a          277 c          274 g          258 t
ORIGIN      
        1 tttcgcagcc atgcggtgta taaaaaatga tctcatgcag atgttttgtg aatgtgtggg
       61 ttgacattca tatgaaaaaa atcataattc catcatgttt gtgtggggtc ttttctgtac
      121 cttacgcatc gcactcaagc ctgacagaaa atatgctgta aggctcatat caaaaaccgc
      181 cattagctca tcaggaagag cagacgacaa ccaggattgt tgtatggtac ggggttcgag
      241 gcctcgatgg cggtccaact tttcaaaact cttctgaatc agatcccctt attatcatcc
      301 ctgcaaagtg gaacattgcc attgtttggc tattcaggac gggtcagacc aatgaaaaaa
      361 gcccacacag gggagagtgg gctgaaatgg gaagctaaag actcaagtaa acttatcgga
      421 aataaggacc acgcattacg ggggctatca tcgccgatag tcgggattcg gcaaatcagg
      481 ttgattacgt agagagtaaa ttatctgctc accgctgcgt caccccttcg taaatcaggc
      541 gtagcgcgaa tacaccaata attgcaccaa taacccgact ggcaacgcgt tgcatacgcc
      601 cataagcacg acgcacagcg ggcaaagaaa acgcctgact aagaaagact cgccagataa
      661 ctgatgcgag cacaatcccc gcccaggcca ttaaacgtgc ccatgttggt gtttcggcat
      721 ttaatgttac tgagaaaata ctgataaaaa ataaaacggt ttgcgggtta gagagatcgg
      781 taattaatcc gcggcgaaaa aagacatacc aggaggcgct aatcggttgt tgtagtgtgc
      841 tcatttgcgg tgttgactgg cggcgcatgc tgcaccacgc aaaccataag agataagcgc
      901 cgccgacgat tctgataagc gaaaaaatct cctcacactg cgttattagc gttgcaagac
      961 caaacaaacc caacccggaa taaaatgcat cgcccagcgc cacgcccagt ccggtcagca
     1021 cccctgcgcg tcgaccggaa gccaggctgg tttgtactac cacaaagaga ttggctcccg
     1081 gattaaaaaa agtaatcacg aacagtccta cggtcaggta aacggcgtgc aaaggatcca
     1141 tagtgatttc atccataaat
//
LOCUS       DAJTZL010000391         1157 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.394, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000391 DAJTZL010000000
VERSION     DAJTZL010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1157
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.394"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            6..407
                     /locus_tag="OO544_004388"
                     /pseudo
     CDS             6..407
                     /locus_tag="OO544_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            572..>1157
                     /locus_tag="OO544_004389"
     CDS             572..>1157
                     /locus_tag="OO544_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708747.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR6769799.1"
                     /translation="MNKVYSLKYCPVTGGLIAVSELARRVIKKTCRRLTHILLAGIPA
                     ICLCYSQISLAGIVRSDIAYQIYRDFAENKGLFVPGANDIPVYDKDGKLVGRLGKAPM
                     ADFSSVSSNGVATLVSPQYIVSVKHNGGYRSVSFGNGKNTYSLVDRNNHPSIDFHAPR
                     LNKLVTEVIPSAVTSEGTKANAYKYTERYTAFYRV"
BASE COUNT          321 a          260 c          276 g          300 t
ORIGIN      
        1 ggtgacgagt ctcatcgtct atggaaacga cattcgtgct ggatgcactg gagcaggcgt
       61 tgtgggcccg tcgcccgtct ggcaccatcc atcacagcga taaaggctct cagtatgtgt
      121 cactggccta taaggcgcga ctaaaagaag caaaactgct ggcatcaaca gggagtactg
      181 gtgactcgta tgacaacgcg atggcagaga tcatcaaggg cctttacaaa gcggaggtaa
      241 tacaccgtaa gagctggaaa aaccggacag aagtggagct ggccacactc acgtgggtgg
      301 actggtataa caatcgacga ttgctggaaa ggctgggcca tatcccaccg gcagaagcag
      361 aaaaagctta ttatgcttcc atcggaaatg atgatctggc agcctgagtt cacagataaa
      421 acactctcca ggaaacccgg ggcggttcag ttcacaaaaa cacattaatg cagtaactat
      481 attttccttt ctggtgataa cgtcgggtta tcattagctt cttcagctat tttactttta
      541 tatcccttgt aaacatcatg gagaatccat agtgaataaa gtttattctc ttaaatattg
      601 ccccgtcacc ggggggctta ttgctgtctc tgaacttgcc cgcagggtaa taaaaaagac
      661 atgccgaaga ttaacgcata ttcttctggc tggcattcca gcaatctgtc tgtgttactc
      721 tcagatatcc ctggcgggta ttgtccgttc cgatattgcc tatcagattt atcgtgattt
      781 cgccgaaaac aaagggcttt ttgtacctgg tgccaatgat attccggtat atgataagga
      841 cggaaaactt gtgggaagac tgggtaaagc cccaatggcc gatttcagca gtgtgagctc
      901 aaatggcgtt gctacgcttg tatcgcctca gtatatcgtc agcgtaaagc ataacggagg
      961 atatcggagt gtgagctttg gtaatgggaa aaatacatat tcccttgttg accgtaataa
     1021 ccacccttct attgacttcc atgctccacg tctgaataaa ctggttacag aagttattcc
     1081 ctcagcggta acatcagaag gaaccaaagc caatgcttat aaatacactg aacgttacac
     1141 cgctttttat cgggtgg
//
LOCUS       DAJTZL010000392         1148 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.395, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000392 DAJTZL010000000
VERSION     DAJTZL010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.395"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            119..376
                     /locus_tag="OO544_004390"
     CDS             119..376
                     /locus_tag="OO544_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6769800.1"
                     /translation="MNNQTMTFTPEQLRKHAQEMLRHAEQLEKTGITKDAIRKDMVPA
                     LRELMQAKHRAQKAVDELVDCVAELETRVGKFEKLVQEVLR"
     gene            376..750
                     /locus_tag="OO544_004391"
     CDS             376..750
                     /locus_tag="OO544_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCR6769801.1"
                     /translation="MRHEFILPYPPTVNTYWRRRDNTYFVSKAGERYRRDVALIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYPIMHEEQVKK"
     gene            747..>1148
                     /locus_tag="OO544_004392"
     CDS             747..>1148
                     /locus_tag="OO544_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCR6769802.1"
                     /translation="MKLEDLPKYYSPKSPCLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCYGEKFIESVVFTNK"
BASE COUNT          325 a          251 c          317 g          255 t
ORIGIN      
        1 gacattttct gcctgtggac caggaaagtg cacgggtgct ttccactccc atgaagcccg
       61 gcaatccgcg cagatacagt aataaacgaa ctgacagcag taagatggag cgtttctttt
      121 gaacaaccag acaatgactt ttacccctga acaattgcgt aaacacgcgc aggaaatgct
      181 gcgtcatgct gaacaactcg aaaaaacggg cataacaaaa gacgctatcc gtaaagatat
      241 ggtacccgcg cttcgggaac tgatgcaggc gaagcatcgc gcacagaaag cagtagatga
      301 gctggtggac tgtgtggcag agctggaaac cagagtcgga aagtttgaaa aactggtgca
      361 ggaggtgctg cgctgatgcg ccatgagttt attttacctt atccgccgac ggtgaatact
      421 tactggcgac gtcgtgacaa cacatatttt gtatcaaaag ccggtgagcg ttatcgccgg
      481 gatgtggcgc ttattgtccg tcagcagcga ctgaaattaa gcctgtccgg aagactggcg
      541 ataaagatta ttgcagagcc accggataag cgccgtcgtg acctggacaa tatcctgaaa
      601 gcaccactgg atgcactgac acatgcggga gtgttaatgg acgatgagca gtttgatgaa
      661 atcaatatcg ttcgtggtca gccagtatct ggtggacgtc tgggggtgaa gatttacccc
      721 ataatgcatg aagagcaggt caaaaaatga aactggaaga tttaccgaaa tactactccc
      781 caaaatcccc ttgcctgacc gatgcatcgg cctcaacgtc aaaagatgcg ctgagtatca
      841 ctgatgtgat ggccgcgcag ggcatgacac agaatcgggc tgagatgggg ttttctgcgt
      901 tcctggggaa aatgggcatc agtatgaatg acagggcgcg ggcaacagaa ttactggcag
      961 attatgcact cagtcggtgc gatcgtgtgg cggcgttgag aaaacttccg gcagaaataa
     1021 aaccggtagt gatgcgcatt atggcttcgt acgcttttga ggattatgcc cgcagcgcag
     1081 cgagtaaaaa gcagtgccct tgttgctatg gggaaaaatt tattgaaagc gtagttttta
     1141 caaacaag
//
LOCUS       DAJTZL010000393         1145 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.396, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000393 DAJTZL010000000
VERSION     DAJTZL010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1145
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.396"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..187
                     /locus_tag="OO544_004393"
     CDS             <1..187
                     /locus_tag="OO544_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR6769803.1"
                     /translation="REYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNN
                     YRIALRKYQHYQRQTCT"
     gene            320..1120
                     /gene="tcyJ"
                     /locus_tag="OO544_004394"
     CDS             320..1120
                     /gene="tcyJ"
                     /locus_tag="OO544_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6769804.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTADDLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
BASE COUNT          318 a          248 c          306 g          273 t
ORIGIN      
        1 gcgtgaatat gtcgttcgtc tgcgccgttt gggtaatcat ctacacgagc aaaatatttc
       61 cctcgatcag ctgcaggacg gtttccttga tgaaatcctc gccccgtggc tgcccaccac
      121 cagcaccaac aattaccgca ttgcgttacg gaagtatcaa cactatcagc gtcaaacgtg
      181 tacctgactt gtgcagaaat cctccagtct gcctgcttct gatatatatt aaataagaat
      241 aagatgtagc ggagttgttt ttgtgtttac aaacaatggc tctacactgc aaacagacat
      301 aacaacattc ggggtgaata tgaaattagc acatctggga cgtcaggcat tgatgggtgt
      361 gatggccgtg gcgctggttg cgggtatgag cgttaaaagt tttgcagatg aaggtctgct
      421 taataaagtc aaagagcgcg gcacgctgct ggtagggctg gaaggaactt atccgccgtt
      481 cagttttcag ggggatgacg gcaaattaac cggttttgaa gtggaatttg cccaacagct
      541 ggcaaaacat cttggcgttg aggcgtcact aaaaccgacc aaatgggacg gtatgctggc
      601 gtcgctggac tctaaacgca ttgatgtggt gattaaccag gtcaccattt ctgacgagcg
      661 caagaaaaaa tacgattttt ctacccctta caccatttcc ggtattcagg cgctggtgaa
      721 aaaaggtaac gaaggcacca ttaaaacagc cgatgatctg aaaggcaaaa aagtgggtgt
      781 cggtctgggc accaactatg aagagtggct gcggcagaat gttcagggtg tcgatgtgcg
      841 tacctatgat gatgacccga ccaaatatca ggatctgcgc gtaggccgta tcgatgcgat
      901 cctcgttgac cgtctggcgg cgctggatct ggtgaagaaa accaacgata cgctggcagt
      961 aaccggtgaa gcattttccc gtcaggagtc tggcgtggcg ctgcgtaaag gaaatgaaga
     1021 tctgctgaaa gcagtgaatg atgcaattgc ggaaatgcaa aaagatggca ctctgcaagc
     1081 cctttccgaa aaatggtttg gtgctgatgt gaccaaataa tcagcataat gacaaaaaag
     1141 ggcgc
//
LOCUS       DAJTZL010000394         1117 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.397, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000394 DAJTZL010000000
VERSION     DAJTZL010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1117
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.397"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            84..1115
                     /locus_tag="OO544_004395"
     CDS             84..1115
                     /locus_tag="OO544_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630-like element IS630 family transposase"
                     /protein_id="HCR6769805.1"
                     /translation="MPIIAPISRDERRLMQKAIHKTHDKNYARRLTAMLMLHRGDRVS
                     DVARTLCCARSSVGRWINWFTQSGVEGLKSLPAGRARRWPFEHICTLLRELVKHSPGD
                     FGYQRSRWSTELLAIKINEITGCQLNAGTVRRWLPSAGIVWRRAAPTLRIRDPHKDEK
                     MAAIHKALDECSAEHPVFYEDEVDIHLNPKIGADWQLRGQQKRVVTPGQNEKYYLAGA
                     LHSGTGKVSCVGGNSKSSALFISLLKRLKATYRRAKTITLIVDNYIIHKSRETQSWLK
                     ENPKFRVIYQPVYSPWVNHVERLWQALHDTITRNHQCSSMWQLLKKVRHFMETVSPFP
                     GGKHGLAKV"
BASE COUNT          303 a          285 c          301 g          228 t
ORIGIN      
        1 tagatcactt tgagggaact tagcccggat tgtgcgatct gatcaatcgc caaatcaaaa
       61 caaatcacca accggactga gcaatgccga tcatagcacc aatttcccgt gacgaacgac
      121 gcctgatgca gaaagccatc cataaaacac acgataaaaa ttatgcccgc agactgactg
      181 ccatgctgat gctgcaccgg ggcgaccgtg tcagcgacgt tgccagaacg ctctgctgcg
      241 cccgttcctc tgttggacgc tggattaact ggttcacgca gtcgggtgtt gagggactga
      301 aatcattacc tgccgggcgt gcccgtcgct ggccgtttga gcatatctgc acactgttac
      361 gtgagctggt aaaacattct cccggcgact ttggctacca gcgttcacgc tggagtacag
      421 aactgctggc aataaaaatc aatgagataa ccggttgcca gttaaatgcc ggaaccgttc
      481 gccgctggtt gccgtctgcg gggattgtgt ggcgaagggc tgcgccaact ctgcgtatcc
      541 gtgacccgca taaagatgaa aagatggcag caatccataa agcactggac gaatgcagcg
      601 cagagcatcc ggtcttttat gaagatgaag tggatatcca tcttaatccc aaaatcggtg
      661 cggactggca actgcgcgga cagcaaaaac gggtggtcac gccgggacag aatgaaaaat
      721 attatctggc cggagcgctg cacagcggga caggtaaagt cagctgtgtg ggcggcaaca
      781 gcaaaagttc ggcgctgttc atcagcctgc tgaagcggct taaagcgaca taccgtcggg
      841 cgaaaaccat cacgctgatc gtggacaact acattatcca caaaagccgg gaaacacaga
      901 gctggctgaa ggagaacccg aagttcaggg tcatttatca gccggtttac tcgccatggg
      961 tgaatcatgt tgaacggcta tggcaggcac ttcacgacac aataacgcgt aatcatcagt
     1021 gcagctcaat gtggcaactg ttgaaaaaag ttcgccattt tatggaaacc gtcagcccat
     1081 tccccggagg caaacatggg ctggcaaaag tgtagcg
//
LOCUS       DAJTZL010000395         1106 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.398, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000395 DAJTZL010000000
VERSION     DAJTZL010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.398"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..613
                     /locus_tag="OO544_004396"
                     /pseudo
     CDS             1..613
                     /locus_tag="OO544_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417591.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          398 a          211 c          190 g          307 t
ORIGIN      
        1 atgttccagg ttaattttgt tagttcaaca caaaacgcca caatctcctg ggattattta
       61 ctaaaaataa cgcctgtttt aataagcatt agcgatatgt attctgaaga aaaaatcaag
      121 tttgtcgaaa gttgtttaaa tgagcttgga gacattaccg aagaacaatt aaaaattatg
      181 agatttgcaa ttataaaatc tataccaagg gcaactctta cagataaatt agaaaatgaa
      241 ttaacaaaag aaatatataa aagctcatcg aaaatcaaca attgcttgaa cagaattaaa
      301 ttaccagaga tgaaagaatt ctcatcagaa aaaaatatat gattacatcg atataatcat
      361 tgaagattat gaaaatatca aagaaaatgc ttatctggtc gatccaaaaa ttaattacac
      421 tatagattta aacatagaag actctagctc agaggaattc ctttttgata atagcctcga
      481 gaaagacgaa aattcttcgg acaattgctt tgaggtcgaa aaatataacg catatgaagc
      541 atataactca gagaatctat attttaccag agaggaatat acgtatgatt acgacctttt
      601 aaatgcaata tagacataat aacttcttac aaaatctata attaataccg tagatattca
      661 ttcatcttac cctgtgcacc aaaagcaaaa actcactgac gcgggtttga aactgatggc
      721 aaccgcaaaa atgcctgatg cgctacgctt atcaggccta cgccatctct acaatatatt
      781 gaatttgcgt gcttttgtag gcaggataag gcgttcacgc agcatccggc atgaataaag
      841 cgcactcaac aatctgaaac ccgccggaac ggtttattac gtacatcagg tgaaactgac
      901 cgataagccg ctttcttttg ggtatagtgt cgtggacagt cattcatctt tctgcccctc
      961 caaaagcaaa aacccgccga agcgggtttt tacgtaaatc aggtgaaact gaccgataag
     1021 ccgctttctt ttgggtatag cgtcgtggac agtcattcat ctttctgccc ctccaaaagc
     1081 aaaaacccgc cgaagcggtt ttttac
//
LOCUS       DAJTZL010000396         1096 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.399, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000396 DAJTZL010000000
VERSION     DAJTZL010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1096
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.399"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..1074
                     /locus_tag="OO544_004397"
     CDS             join(<1..167,167..1074)
                     /locus_tag="OO544_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS600 family transposase"
                     /protein_id="HCR6769806.1"
                     /translation="EGASRLSLPEGTLGQWVTAARKGLGTPGSRTVAELESEILQLRK
                     ALNEARLERDIFKKSNSVFCTGVAEKYALIEQWRQQFPIEAMCQVFGVSRSGYYNWVQ
                     HEPSDRKQSDERLKLEIKVAHIRTRETYGTRRLQTELAENGIIVGRDRLARLRKELRL
                     RCKQKRKFRATTNSNHNLPVAPNLLNQTFAPTAPNQVWVADLTYVATQEGWLYLAGIK
                     DVYTCEIVGYAMGERMTKELTGKALFMALRSQRPPAGLIHHSDRGSQYCAYDYRVIQE
                     QSGLKTSMSRKGNCYDNAPMESFWGTLKNESLSHYRFNNRDEAISVIREYIEIFYNRQ
                     RRHSRLGNISPAAFREKYHQMAA"
BASE COUNT          312 a          261 c          286 g          237 t
ORIGIN      
        1 tgaaggcgct tcccgattat ctcttcctga aggcacttta ggacaatggg ttaccgccgc
       61 cagaaaaggg ctcggtactc ctggttcccg cacggtggct gaactggaat ctgaaattct
      121 gcaactgcgt aaggcgttaa atgaagctcg ccttgagcga gatatattaa aaaaagcaac
      181 agcgtatttt gcacaggagt cgctgaaaaa tacgcgttaa tcgaacaatg gcgacaacaa
      241 tttcccattg aagcgatgtg tcaggtattt ggtgtatcca ggagcggtta ttacaactgg
      301 gtacagcatg aaccctcaga cagaaaacaa agtgatgagc ggctaaaact ggagattaag
      361 gtggcacata tccgcactcg cgaaacatat ggaacccggc ggctccagac ggagctggca
      421 gagaatggca tcatcgttgg tcgtgaccga ctggcacgtc ttcgtaagga gctaaggcta
      481 cgctgtaagc agaaacgcaa gttcagagcg actacgaact cgaaccacaa tctgccagtt
      541 gcgccaaatc tgctgaacca gacgttcgct cctacagcac caaatcaggt ctgggtggcg
      601 gacctgacgt atgttgccac acaggaggga tggttgtacc tcgctggcat caaagatgtt
      661 tatacgtgcg aaattgtcgg ctacgccatg ggagagcgca tgacaaaaga gctgacaggt
      721 aaagccctgt ttatggcgct caggagccag cgcccacctg ccgggctaat ccaccactct
      781 gatcgaggtt cacagtactg cgcatacgat taccgggtca tacaggagca gtctggtctg
      841 aaaacatcaa tgtcgcgtaa aggtaactgt tacgacaacg ctccgatgga aagcttctgg
      901 ggaacgctga aaaatgagag cctgagccac tatcgtttta ataaccggga tgaagccatc
      961 tcagtaatac gggaatacat tgagattttc tacaatcgtc agcgtcgtca ctctcgtctg
     1021 gggaatatct ccccggcagc cttcagggaa aaatatcatc agatggctgc ttaaaaaaag
     1081 aacaaatggt agtgtc
//
LOCUS       DAJTZL010000397         1088 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.400, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000397 DAJTZL010000000
VERSION     DAJTZL010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1088
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.400"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            212..>1088
                     /gene="nadA"
                     /locus_tag="OO544_004398"
     CDS             212..>1088
                     /gene="nadA"
                     /locus_tag="OO544_004398"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HCR6769807.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMLTLQAECSLDLGCPVEEFNAFCDAHPDHTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSFGEKIIWAPDKHLGRYVQKQTGADILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKTLPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCR"
BASE COUNT          294 a          259 c          265 g          270 t
ORIGIN      
        1 caggcatcct caatttcact ttgtaaacct gatgacatcg tcagagctta ctgtgcaagc
       61 aactctacgt cggtggaatt aggcgtaatt cgagtgactt ttccccacca ttcgactatc
      121 ttgtttagca tataaaacaa attacgccga tagtagcgaa tattacgcta atgtcggttt
      181 taacgttaag cctgtaaaac gagatggtaa gatgagcgtg atgtttgatc cagacacggc
      241 gatttatcct ttccccccga agccgacgcc gttaagcatt gatgaaaaag cgtattaccg
      301 cgagaagata aaacgtctgc taaaagaacg taatgcggtg atggttgccc actactatac
      361 cgaccccgaa attcaacaac tggcagaaga aaccggtggc tgtatttctg attctctgga
      421 aatggcgcgc ttcggtgcaa agcatcccgc ttctacgttg ttagtcgctg gggtgagatt
      481 tatgggagaa accgccaaaa ttctcagtcc ggaaaaaaca attctgatgc tgacacttca
      541 ggctgaatgt tcactggatc tcggctgccc tgttgaagaa tttaacgcat tttgcgatgc
      601 ccatcccgat catactgtcg tcgtctacgc caacacttct gctgcggtaa aagcgcgcgc
      661 agattgggtg gtaacttcaa gcattgccgt cgaacttatt gatcatctgg atagttttgg
      721 tgaaaaaatc atctgggcac ccgacaaaca tctggggcgt tacgtgcaaa aacagacggg
      781 cgcagacatt ttatgctggc agggtgcctg tattgtgcat gatgaattta agactcaggc
      841 gttaacccgc ttgcaagaag aatacccgga tgctgccata ctggtgcatc cagaatcacc
      901 acaagctatt gtcgatatgg cggatgcggt cggttccacc agtcaactga tcgctgctgc
      961 gaaaacattg ccacatcagc ggcttatcgt ggcaaccgat cggggcattt tctacaaaat
     1021 gcagcaggcg gtgccagata aagagttact ggaagcacca accgcaggtg agggtgcaac
     1081 ctgccgca
//
LOCUS       DAJTZL010000398         1085 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.401, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000398 DAJTZL010000000
VERSION     DAJTZL010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1085
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.401"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..643)
                     /locus_tag="OO544_004399"
     CDS             complement(<1..643)
                     /locus_tag="OO544_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR6769808.1"
                     /translation="MSKISDLNYSQHITLADNFKQKSEVLNTWRVGMNNFARNAEGQD
                     NTRNILDPKTFLEFLVKIFTLGYVDFSKRSNEAGRNMMAHIESSSYIKNNDGSEIMKF
                     VMNNPEGERADLSKVEIEITLSAFTTMGTRQGHTAIIFQQPDGSTNRYEGKSFERKDE
                     SSLHLITNKILACYQREANKEIARLLNIPQELNNSQDLNNSQVSCKDSVDSTIT"
     gene            complement(766..>1085)
                     /gene="tabA"
                     /locus_tag="OO544_004400"
     CDS             complement(766..>1085)
                     /gene="tabA"
                     /locus_tag="OO544_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HCR6769809.1"
                     /translation="YLISEDMTEPHEARRTEYHARYLDIQIVLKGQEGMTFSTQPAGT
                     PDTDWLADKDIAFLPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKM
                     LMA"
BASE COUNT          312 a          247 c          191 g          335 t
ORIGIN      
        1 ccgtaatcgt agaatcaact gaatctttac aactcacctg cgagttattc aagtcctgag
       61 aattatttaa ctcctgcgga atatttaata gacgagctat ttccttgtta gcttcgcgtt
      121 ggtaacatgc cagaatcttg ttagtaatca ggtgtaatga actctcatct tttctttcaa
      181 aggactttcc ttcataacga ttagtcgaac catcaggttg ttgaaatata atagccgtat
      241 gtccttgacg agtccccata gtagtgaaag cggaaagtgt gatttctatt tccaccttag
      301 ataaatccgc tcgttcccct tcaggattat tcataacaaa cttcattatc tcactgccat
      361 cattattttt gatataagat gaggactcaa tatgagccat catatttctt cccgcttcgt
      421 tggagcgttt gctaaaatcc acataaccta gagtaaatat ttttaccaaa aattccaaaa
      481 atgtcttagg atcaaggata tttcttgtgt tatcctgccc ctcggcatta cgggcaaaat
      541 tattcattcc aacacgccag gtatttaaaa cttcactttt ttgtttaaaa ttgtcggcta
      601 atgtaatgtg ttgagaataa ttcaaatctg atattttgct catgacgaac tccttgagtt
      661 aataagaacg tctaagcatt acaaatttta ctcacaaaaa aatatcaaaa aacgctcttt
      721 tcacaaactc gccgtaaacc tggagttatt tacggcgaaa agtcatcacg ccatcagcat
      781 cttcactact gctttgcgaa cctgggctgg tgcacccact gcgcacagcg gtttatgcac
      841 ttcccccgga taaaacacaa caaaatcacc ttcattaagg ataactgttt tctcatcaac
      901 gccttccggc aaaaatgcga tgtctttatc agctaaccag tcggtatccg gcgtgcctgc
      961 aggttgcgtg ctgaaggtca tgccttcctg accttttaac acaatctgaa tgtcgagata
     1021 gcgggcatgg tactccgtac ggcgagcttc gtgcggctcg gtcatatctt ccgagataag
     1081 ataaa
//
LOCUS       DAJTZL010000399         1045 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.402, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000399 DAJTZL010000000
VERSION     DAJTZL010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1045
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.402"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            284..388
                     /locus_tag="OO544_004401"
                     /pseudo
     CDS             284..388
                     /locus_tag="OO544_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            728..985
                     /locus_tag="OO544_004402"
     CDS             728..985
                     /locus_tag="OO544_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm development YmgB/AriR family protein"
                     /protein_id="HCR6769810.1"
                     /translation="MHSDYYNMVFGEKLANILYEANSQFFYERNVIEEAVNALFCERE
                     IINNKNIIKKLMFFLSDVNHTKKDVVQSALNIIIDITSGDI"
BASE COUNT          358 a          178 c          198 g          311 t
ORIGIN      
        1 atcatacaga taattactgg ttaaccctat atagggcatt ttaaagtgtt gtgatcttac
       61 ccagcaatag tggacacgcg gctaagtgag taaactctca gtcagaggtg actcacaaga
      121 caaaacagtt tcaaccagta aaaaaacgta aacaccattc gcatgaattt cgtagttaag
      181 atctgaagct tgcagaatgc atcggtgtta ctgccggagt ccgtgaactt agtctgcatg
      241 aatcaccaca gctttacatc tgtcgcagta aacagcaaaa tcagcaaact tacttaatta
      301 tattgagtgc gattacaatc gctggcgatg gtacagttgg tgtggcggtc tcagtccgga
      361 acaatttgaa aatcagaatc tcacttaaag cggcatccat tttacaaaag atggccgtct
      421 gtgtatttgt aatgaatacc ttctacatgt ttagagtgca taatccacga caaggcgata
      481 tcctaaaaat tagctagcag gcaactttga tggcatagat aatgttttta gtaaaaaaga
      541 gttgactgaa tataatacag cctcttccta cataagaagg aatgtgctca caaaaaaaaa
      601 acattagtct gcttcctgtt cttacaataa tcagtattat gtattttatt tatatagcaa
      661 aatatgcttg ttacacctgt cataattagg catggtaaaa ttattgactg aaccctgggg
      721 gaggaagatg cacagtgatt attataatat ggtttttggt gaaaaattgg ccaatatttt
      781 atatgaggct aactctcaat ttttttacga gagaaatgtc atagaagagg cagtcaacgc
      841 tttattctgt gagcgtgaaa taataaacaa caagaacata ataaagaagc tgatgttctt
      901 tttgtcagat gttaatcaca ccaagaaaga tgttgttcag agcgcactta atattatcat
      961 tgacattaca tcaggtgata tttgagattt tacataaaag ataatctctc aaggagtctt
     1021 atctgaaaac gaaatatata gtccc
//
LOCUS       DAJTZL010000400         1023 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.403, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000400 DAJTZL010000000
VERSION     DAJTZL010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.403"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(77..865)
                     /locus_tag="OO544_004403"
     CDS             complement(77..865)
                     /locus_tag="OO544_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR6769811.1"
                     /translation="MSIIEVTGNPRHDQLVHLIAERGYMNIEELAQLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRVSSVMNTAFEQRELSLTAEKRAIAEAVADYLPERCTVFI
                     TIGTTVEAVARALLNRRDLRIITNSLRVAQILYKNQDIEVMVPGGTLRAHNGGIIGPG
                     AVDFIEGFRADYLITSIGAIEHDGTLLEFDVNEALVARTMIKHARNTLLVADHTKFAA
                     SAAVSIGNARNVRAFFTDAPPPNSFCQLLSEENVELVVAEQEVS"
     gene            complement(905..>1023)
                     /locus_tag="OO544_004404"
     CDS             complement(905..>1023)
                     /locus_tag="OO544_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HCR6769812.1"
                     /translation="KEAIRFASAVAAMKCTQPGGRAGIPNREQTESFLSLYA"
BASE COUNT          255 a          263 c          239 g          266 t
ORIGIN      
        1 atcgctggca ggtttttttt gcttatggaa aacaaaatcc gctgaaattc ccagcggatt
       61 ttccgaaatt tcaatttcag gatacttctt gctcggcaac caccagttca acattctctt
      121 cacttaacaa ctggcagaaa gaattgggag gcggggcatc agtaaaaaaa gccctgacat
      181 tccgtgcatt gccaattgaa acggcagcag acgcggcaaa cttcgtatga tcggccacta
      241 acaatgtatt ccgcgcatgt ttaatcatcg ttctcgcgac taacgcttca tttacatcaa
      301 attccagtag ggtgccgtca tgttcaatgg cacctatact ggtgattaaa taatctgcgc
      361 ggaagccttc aataaaatcc acggctcctg ggccgataat cccgccgtta tgagcgcgta
      421 aagtgcctcc cggcaccatc acttcaatat cctgattctt ataaagaatc tgtgccacac
      481 gtaggctgtt agtgataatg cgtaaatcac gccggttgag taatgccctg gcaacggctt
      541 ctacggttgt accgatggtg ataaagacgg tacagcgttc gggaaggtaa tcggcgactg
      601 cctcagcgat cgcccgtttc tcggcggtga gcgaaagttc ccgttgctca aaagcggtat
      661 tcatgacgct ggagacgcgc ccggcccctc catgatgacg ggtgatcaag ccctgctcac
      721 tgagttttcg gatatcccgg cgcacggttt gcgttgaaac atccagcagt tgtgccagtt
      781 cttcaatatt catataaccg cgttctgcga ttagatgaac gagttggtca tggcgtggat
      841 tacctgtcac ttcgatgata ctcatgaata ctcccttgcc cctcctcggt agatgtttac
      901 cattttacgc ataaagtgac aaaaatgatt cggtttgctc acgattagga atgccggccc
      961 gaccgccagg ctgggtacat ttcatcgcgg caaccgcact ggcaaaacga atagcctctt
     1021 ttg
//
LOCUS       DAJTZL010000401         1003 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.404, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000401 DAJTZL010000000
VERSION     DAJTZL010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1003
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.404"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>1003
                     /locus_tag="OO544_004405"
     CDS             <1..>1003
                     /locus_tag="OO544_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR6769813.1"
                     /translation="FHNVTAEQIAYVAQLQRSGDEAGALQAANEAATKGFDDQTRRLK
                     ENMGTLETWADRTARAFKSMWDAVLDIGRPDTAQEMLIKAEAAFKKADDIWNLRKDDY
                     FVNDEARARYWDDREKKRLERDAAQKRVDQQRQQDKNAQQQSDTEASRLKYTEEAQKA
                     YERLQTPLEKYTARQEELNKALKDGKILQADYNTLMAAAKKDYEATLKKPKQSGVKVY
                     AGDRQEDSAHAALLTLQAELRTLEKHAGANEKISQQRRDLWKAESQFAVLEEAAQRRQ
                     LSAQEKSLLAHKDETLEYKRQLAALGDKVTYQEHLNALAQQADKFAQQQRAKRAAIDA
                     "
BASE COUNT          287 a          236 c          325 g          155 t
ORIGIN      
        1 agttccataa cgtgacggcg gagcagattg cgtatgttgc tcagttgcag cgttccggcg
       61 atgaagccgg ggcattgcag gcggcgaacg aggccgcaac gaaagggttt gatgaccaga
      121 cccgccgcct gaaagagaac atgggcacgc tggagacctg ggcagacagg actgcacgtg
      181 cattcaaatc catgtgggat gcggtgctgg atattggtcg tcctgatacc gcgcaggaga
      241 tgctgattaa ggcagaggct gcgtttaaga aagcagacga catctggaat ctgcgcaagg
      301 atgattattt cgttaacgat gaagcacggg cgcgttactg ggatgatcgt gaaaaaaaac
      361 gcctggagcg ggatgcagcc caaaagaggg tagatcaaca gcgtcaacag gacaaaaatg
      421 cgcagcagca gagcgatacc gaagcgtcac ggctgaaata taccgaagag gcgcagaagg
      481 cttacgaacg gctgcagacg ccgctggaga aatataccgc ccgtcaggaa gaactgaaca
      541 aggcactgaa agacggaaaa atcctgcagg cagattacaa cacgctgatg gcggcggcga
      601 aaaaggatta tgaagcgacg ctgaaaaagc cgaaacagtc cggcgtgaag gtgtatgcgg
      661 gcgatcgtca ggaagacagt gctcatgcgg ccctgctgac gcttcaggca gaactccgga
      721 cgctggagaa gcatgccgga gcgaatgaga aaatcagcca gcagcgccgg gatttgtgga
      781 aggcagaaag tcagttcgcg gtactggagg aggcggcaca acgtcgccag ctgtccgcac
      841 aggagaaatc cctgctggcg cataaagacg agacgctgga gtacaaacgc cagctggctg
      901 cacttggtga caaggttacg tatcaggagc acctgaatgc gctggcgcag caggcggata
      961 aattcgcaca gcagcaacgg gcaaaacggg cagccattga tgc
//
LOCUS       DAJTZL010000402         1003 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.405, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000402 DAJTZL010000000
VERSION     DAJTZL010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1003
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.405"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..821
                     /locus_tag="OO544_004406"
                     /pseudo
     CDS             1..821
                     /locus_tag="OO544_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001763881.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="RHS domain-containing protein"
BASE COUNT          293 a          194 c          278 g          238 t
ORIGIN      
        1 ggagcagatg gaaagccaga tggaggcgga atacatcccg gagcggaggc tgcatcttta
       61 ccactgtgac caccggggac tgccgcaggc gctcatcagc ccggaagggg aaacggcgtg
      121 gcgcggggag tatgacgagt gggggaacca gcttaatgag gagaacccgc atcacctgta
      181 ccagccgtac cgtttgccgg ggcagcagta tgatgaggag tcgggactgt attacaaccg
      241 tcaccggtac tacgatccgt tgcaggggcg gtatatcacc caggatccga tagggttgaa
      301 ggggggaata aatctataca cgtatccact ggcaccaata cgttacacca ctggggttag
      361 aacgagttat aagtgtatat gggcctcccg ctccagaccg ggcaggtgct gaaactcctc
      421 ttgttcttac tgatatgact ggaggagtga ctatttatta tgatcctgaa acaggtgatt
      481 caatgacttt tgatagtagc aatagaattg atcgtcgttc tcaacgtggt gctggtgatc
      541 catatacagg tgaagtcgtt ggttgtgaga caaatggatc aggaatatcg gcagcatatg
      601 gcacaacgaa gatctatact acagatacta gagcaagatg gctacatggt gggggatcat
      661 cgttgcgcga tccttatgct cctcgacaag gctggaaacc aactatggga tgtacccgtg
      721 cacagaatga agatgtagat gagttgtgta agaaagtgac aagttggatg tattcgcatc
      781 ctggagagag aattagatat gaacgtttta agacacgtta atgcgattaa catgggagtg
      841 tgcattctaa ttttggttat tggatgtgca aatgctaaag ttcttaaacg atgtatatta
      901 cccgctcctg atggtggcgt agaaaaacta tcaaaaccca acttacatgg agtaattaaa
      961 aaagttgatt tgaacacaaa tgaagcggaa attaaaataa aag
//
LOCUS       DAJTZL010000403          999 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.406, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000403 DAJTZL010000000
VERSION     DAJTZL010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..999
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.406"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>999
                     /locus_tag="OO544_004407"
     CDS             <1..>999
                     /locus_tag="OO544_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin-like protein
                     YeeJ"
                     /protein_id="HCR6769814.1"
                     /translation="SGNLIEGLTVYFALKSGSATLTSLTAVTDQNGIATTSVKGAMTG
                     SVTVSAVTTAGGMQTVDITLVAGPADASKSVLKNNRSSLKGDFTDSAELHLVLHDISG
                     NPIKVSEGLEFVQSGTNVPYVQVSAIDYSKNFSGEYKATVTGGGEGITTLIPVLNGVH
                     QAGLSTTIQFTRAEDKIMSGTVSVNGTDLPTTTFPSQGFTGAYYQLNNDNFAPGKTAA
                     DYEFSSSASWVDVDATGKVTFKNVGSNSERITATPKSGGPSYVYEIRVKSWWVNAGEA
                     FMIYSLAENFCSSNGYTLPRANYLNHSSSRGIGSLYSEWGDMGHYTTEAGFQSNMYW"
BASE COUNT          293 a          220 c          253 g          233 t
ORIGIN      
        1 agtggtaacc tgatcgaagg tctcactgtg tactttgcct taaaaagcgg ctctgccaca
       61 ttaacgtcat taacagcggt gaccgatcaa aacggaatcg cgacaacaag cgtgaaagga
      121 gcgatgacag gtagcgtcac ggtaagcgca gtcacgaccg ctggtggaat gcaaacagta
      181 gatataacgc tggtggcagg cccagcagac gcctcaaagt cagtccttaa gaacaatcgg
      241 tcatcattga aaggagactt taccgatagt gctgagctac atcttgttct gcacgatata
      301 tcaggcaatc cgatcaaagt ttctgaaggg ctggaatttg tacagtcagg taccaacgtg
      361 ccctatgtgc aagttagtgc aattgactac agtaaaaatt tctcaggcga gtacaaagcc
      421 actgttacag gaggcggaga gggtatcaca acgctgatcc ctgtattgaa tggtgttcat
      481 caagctggtc tgagtaccac aatacaattc actcgcgcag aagacaaaat aatgagcggt
      541 acagtatcag tcaatggtac tgacctaccg acaactacat tcccttcgca ggggttcacc
      601 ggggcgtatt atcagttgaa taatgacaac tttgccccag gaaaaacggc ggctgattat
      661 gagttttcaa gctctgcctc ctgggtcgat gttgatgcta ccggtaaagt gacatttaaa
      721 aatgtcggca gcaattcgga aaggattacg gcgacaccaa aatcaggagg ccctagctat
      781 gtatacgaaa tccgtgtgaa gagttggtgg gtgaacgccg gcgaggcttt catgatatac
      841 agccttgctg aaaatttttg cagcagcaat ggctacacgc tccccagagc aaactattta
      901 aaccacagta gttcccgagg catcgggtca ctgtacagtg aatggggaga tatggggcat
      961 tacacgactg aagctggttt tcaatcaaat atgtattgg
//
LOCUS       DAJTZL010000404          957 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.407, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000404 DAJTZL010000000
VERSION     DAJTZL010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..957
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.407"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..395
                     /locus_tag="OO544_004408"
     CDS             <1..395
                     /locus_tag="OO544_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tail tape measure protein"
                     /protein_id="HCR6769815.1"
                     /translation="INAIVKPFYERQMSKSDNPIDKVVRSKVEPTDQALTGDMNSLAA
                     VLWAMVQGKSKDVPQFSPEALNNLKGRVETFTQDLKVIKPSVNIQPQTINITTQTVLD
                     GKVIDERTTSHINRMQEDTLISSAYPEE"
     gene            569..>957
                     /locus_tag="OO544_004409"
     CDS             569..>957
                     /locus_tag="OO544_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6769816.1"
                     /translation="MFDTKQIRYSGKDGIYFHLRDNVESFLALTATEQMDYDSPMMVT
                     TQAMQSGQTITDNVQRAPKTISISGVVVVDSTGSVLLTRQGQLVETFISTVEQWRDQK
                     QIITIVCRDGIAITDSIITQFKASKEHT"
BASE COUNT          327 a          177 c          188 g          265 t
ORIGIN      
        1 tgattaatgc cattgtcaaa ccgttctatg aacgtcagat gagtaagtca gataatccaa
       61 ttgataaagt tgtaaggtca aaggtggaac ccacagacca agcattgact ggtgacatga
      121 actctttagc cgctgtttta tgggctatgg ttcagggcaa atcaaaagat gttccacagt
      181 tttcacctga agcattaaat aatctgaaag gtcgagtgga aacattcaca caagatttaa
      241 aggttatcaa accgtcagta aatattcagc ctcagacaat caatatcaca actcagacgg
      301 tacttgatgg aaaggttatt gatgaaagaa ccacatcgca tattaatcgt atgcaggaag
      361 ataccttgat tagctcagct tatccagagg aataaacaat gttagagcta ctaatattta
      421 ttgccacaca tggcaatcta ttaatcttta tctagtatga cgattcaact atgaatagtc
      481 gaatgctacg tgaaatcttc tgaacagata ccaacatcaa aaattagatt tcacgtagct
      541 cacgcttaac agaataggaa taacaacaat gtttgacaca aagcaaatta gatactcagg
      601 taaagatggt atctattttc accttagaga taatgtggaa tcttttcttg ccttaacagc
      661 tactgaacag atggactatg actcaccgat gatggtgaca actcaggcta tgcaatcggg
      721 gcaaactatc actgataatg ttcagagagc acctaagacc atctctatta gcggtgttgt
      781 agtggttgat tcgacgggtt cagttctatt aaccagacag gggcaacttg tagaaacctt
      841 tatttctacg gttgaacaat ggcgagacca gaagcaaatc atcactattg tttgtagaga
      901 tggaatagct atcactgata gtatcattac tcagtttaaa gcgagtaaag agcatac
//
LOCUS       DAJTZL010000405          933 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.408, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000405 DAJTZL010000000
VERSION     DAJTZL010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..933
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.408"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(195..>933)
                     /locus_tag="OO544_004410"
     CDS             complement(195..>933)
                     /locus_tag="OO544_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCR6769817.1"
                     /translation="GPTLTFLFVSPLLNPIIVGLMWMTFGWKVTLLYAIIAAGVSVLA
                     SIILDSLGFERHIIASKSSSANCCAPAKTSPGTTYTPIKVSCCSPAAKAIENPVVTCC
                     STKAVVSINPIKLATKDALQQFKDVLPYLLLGVLIGSFIYGFIPSAWIAAHAGADNPF
                     AIPLSAVVGIPLYIRAEAVIPLASVLMTKGMGLGALMALIIGSAGASLTEVILLKSMF
                     RMPMIAAFLTVILGMAILMGYLTQLLF"
BASE COUNT          279 a          198 c          199 g          257 t
ORIGIN      
        1 gcttctccga taaaatgtca gagaagcatg attacgtggt tattgtctgt tgatgacttg
       61 ttgaccgagc cgtcatcatt ctcaatgcat tagttaaatc atttgtgatt tttaaggatt
      121 atcctgtcta tgattttcta cttagaagga tatggcacta actatgcatc ttatagttta
      181 ctgctggcat ctatttaaaa tagcaattga gtcaaatagc ccatcaagat agccatacct
      241 aatataaccg tcaggaatgc agctatcatc ggcattctga acattgattt aagtaatatc
      301 acttccgtca ggcttgcgcc ggcactgccg atgattaatg ccattaatgc tcccagaccc
      361 attccttttg tcatcagaac agatgccaga gggataaccg cctcagcccg gatatacagc
      421 ggaataccga caacggcgct taatgggatg gcgaagggat tatctgctcc tgcatgagcg
      481 gcaatccacg ctgagggaat aaagccataa ataaaagatc ctattaatac tcctaacaaa
      541 agatatggca gtacatcttt aaactgttgc aacgcatctt tggtggctag ttttatggga
      601 ttaatactta ctacagcttt ggtactgcaa caggtaacta cggggttctc aatggcttta
      661 gccgctggac tacaacaact cacttttatg ggcgtatatg tcgttcccgg cgaagttttt
      721 gctggagcac aacaatttgc tgatgagctt ttactggcaa tgatatgacg ttcaaacccc
      781 agagaatcca ggataatact ggcaagaacg gagacgccgg ctgcgataat cgcgtacaac
      841 aaggtgactt tccaaccaaa ggtcatccac attagtccga cgataatggg attaagtaat
      901 ggtgaaacaa ataaaaaagt gagagtcgga cca
//
LOCUS       DAJTZL010000406          930 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.409, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000406 DAJTZL010000000
VERSION     DAJTZL010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..930
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.409"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..345)
                     /locus_tag="OO544_004411"
     CDS             complement(<1..345)
                     /locus_tag="OO544_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HCR6769818.1"
                     /translation="MSPSLRKAVAAAIGGGAVAIASVLITGPGGNDGLEGVSYIPYKD
                     IIGVWTVCHGHTGKDIMLGKTYTEAECKALLNKDLATVARQINPYIKVDIPETTRGAL
                     YSFVYNVGAGNFR"
     gene            complement(345..560)
                     /locus_tag="OO544_004412"
     CDS             complement(345..560)
                     /locus_tag="OO544_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HCR6769819.1"
                     /translation="MYQMEKISTGIAYGTSAGSAGYWFLQWLDQVSPSQWAAIGVLGS
                     LLLGLLTYLTNLYFKIREDKRKAARGE"
BASE COUNT          246 a          239 c          195 g          250 t
ORIGIN      
        1 tctgaaattg ccagcgccca cgttgtaaac gaacgagtaa agagcgccgc gcgttgtttc
       61 cggtatatcg actttgatgt acgggttaat ttgtctggcg acagtggcaa ggtctttatt
      121 caggagggct ttgcattctg cttcggtata cgttttaccg agcatgatgt cttttccggt
      181 gtgtccgtga catacagtcc atacaccaat gatatctttg tatggtatgt agctgacacc
      241 ttccagacca tcgttaccac ctgggccagt gattaacaca gatgctatag caacagcccc
      301 accaccaata gcagctgcaa cagccttgcg taatgatggc gacattattc acctctcgca
      361 gccttacgct tgtcttctct tattttgaaa tacagatttg tcagataagt cagaagcccc
      421 agaagcagac ttcccagcac accaatcgca gcccactgtg atggactgac ctgatccaac
      481 cactgcaaaa accagtagcc ggcactgccg gcggaggtgc cgtaggcaat gcctgttgat
      541 attttttcca tctgatacat atcccgcccc ccgacagacc tgtgctatcg gaaagaaaaa
      601 aggccatcag caaaactctg atggcctgaa tcacctttac caatattgta tgaaaaaaca
      661 cgcacgacta attgacaata attttcattt ccattaaaaa agatcgcgta gcattcttaa
      721 ttcatgagga actttacccg ccagcaatct gagtagcgaa agctgttcat ccccaatgtt
      781 tttgctggcg ggtccttttt tctacggtcc tcctccccgg aggggggcat ttcattattt
      841 ttctaatcgt tactggtgag aaggcggaag aaaaagccag ttcttcgccc ctaaataaca
      901 gcatgattcc agtccatgca aaatctgatt
//
LOCUS       DAJTZL010000407          908 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.410, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000407 DAJTZL010000000
VERSION     DAJTZL010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..908
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.410"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..508)
                     /locus_tag="OO544_004413"
     CDS             complement(<1..508)
                     /locus_tag="OO544_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCR6769820.1"
                     /translation="MATTCSVILILEPFDVYFGKEIVFLERGSSVLVDSSSRDFFLTY
                     PERVIMADFGAEFISRYLKANNLRDISDCREYPSYLKINFADFSLIKGLISWANHCAE
                     YIEIFDESIAFTCLSAFSSEKQFGVFLFGCLKSTGAKVKTIIHTDLSAPWRLKDISSR
                     LYLSESLLK"
     gene            850..>908
                     /locus_tag="OO544_004414"
     CDS             850..>908
                     /locus_tag="OO544_004414"
                     /inference="COORDINATES: protein motif:HMM:NF015750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6769821.1"
                     /translation="MASISIRCPSCSATEGVVR"
BASE COUNT          330 a          179 c          135 g          264 t
ORIGIN      
        1 tctttagtaa actttcgctg agatataatc ttgatgatat atccttaaga cgccatggtg
       61 cagataaatc cgtatgaata atcgttttaa ctttagcccc tgtgcttttc aaacatccaa
      121 acagaaatac tccaaattgt ttttcagaag aaaatgcaga gagacatgta aaagcaatag
      181 actcatcaaa aatttctatg tattcagcac agtgattagc ccaactaatt aatcctttaa
      241 ttaaactgaa gtcagcaaag tttattttta aataagatgg atattcccta caatcagaaa
      301 tatcccttaa gttattagct ttcaaatagc gactaataaa ctcagcgcca aaatccgcca
      361 ttatcactct ttcaggatat gtcaggaaaa aatctctaga gctagagtcg acaagtacag
      421 atgaacctct ctccagaaac acaatctctt ttccgaaata aacatcaaag ggctccaaaa
      481 tcaatataac tgaacatgta gttgccatat catccaccca atttaactga aactaggatg
      541 aagtatatgc atataaagtt cactttgcca aacttcagta taaaaaacca cataaaaaat
      601 agggtgtgat aaaaaatacc gtaacaataa agcaaaggat tataaattcc gttacagtta
      661 caaatgatac tcaagaaaca attccttagg aaaaacttat ttacagccaa taagtaagac
      721 acttatatga tatcaagttt tcataaaaca taactaggct aaatagcttc gcctaatacc
      781 gctacacttt tgccggtaat gctgccaact tactgattta gtgtatgatg gtgattttaa
      841 gatgcttgcg tggcttccat ttccatcaga tgtccttcct gctccgctac tgaaggcgtg
      901 gtgcgtaa
//
LOCUS       DAJTZL010000408          906 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.411, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000408 DAJTZL010000000
VERSION     DAJTZL010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..906
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.411"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..752)
                     /gene="ipaH3"
                     /locus_tag="OO544_004415"
     CDS             complement(<1..752)
                     /gene="ipaH3"
                     /locus_tag="OO544_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase IpaH3"
                     /protein_id="HCR6769822.1"
                     /translation="MSIMLPINNNFSLSQNSFYNTISGTYADYFSAWDKWEKQALPGE
                     NRNEAVSLLKECLINQFSELQLNRLNLSSLPDNLPPQITVLEITQNALISLPELPASL
                     EYLDACDNHLSTLPELPASLKHLDVDNNQLTMLPELPALLEYINADNNQLTMLPELPT
                     SLEVLSVRNNQLTFLPELPESLEALDVSTNLLESLPAVPVRNHHSEETEIFFRCRENR
                     ITYIPENILSLDPTCTIILEDNPLSSRIRESLS"
BASE COUNT          267 a          144 c          222 g          273 t
ORIGIN      
        1 gacagagact ccctgatccg tgaggacaga ggattgtctt cgaggatgat agtgcaggtc
       61 ggatcaaggc taagtatatt ttccggaatg tatgtgatgc gattctcgcg gcaccggaaa
      121 aatatctcgg tttcctctga gtgatgattt cttacaggta cggctggtag gctttccaga
      181 agattagtac ttacatcgag cgcttccagt gattcaggta actcagggag aaatgtcagc
      241 tggttatttc ttactgagag cacttccagc gatgtaggta attcaggaag catggttagc
      301 tgattgttat ctgcattaat atattccagc aatgcaggca attcaggaag catggttagt
      361 tggttgttat ctacatcaag atgtttcaga gatgcgggta attcaggaag tgttgacagg
      421 tgattgtcac aggcgtcaag gtattccagc gatgctggca attctggtaa tgatattagg
      481 gcattctgag taatttccag aacagtgatt tgaggtggta agttgtcagg tagcgaggac
      541 agatttaaac gattcagttg aagctcactg aactgattga tgagacattc tttaagtagg
      601 gagaccgctt cattccgatt ttcaccgggg agcgcttgtt tttcccattt atcccatgct
      661 gaaaagtaat cagcatatgt accggaaata gtgttataaa aagaattttg ggacaatgaa
      721 aagttattat ttatcggtaa catgatggac attcagttaa agccgatgga caaatggctt
      781 tgttaaaaga aataagtagg agtttttatg aagttcattc catgaaatga acttcagcac
      841 ttttttatta taaaaggaca aacaaaattg gctgtaaaaa aacgatactg tttcagttaa
      901 ctgtga
//
LOCUS       DAJTZL010000409          873 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.412, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000409 DAJTZL010000000
VERSION     DAJTZL010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..873
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.412"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>873
                     /gene="cadA"
                     /locus_tag="OO544_004416"
     CDS             <1..>873
                     /gene="cadA"
                     /locus_tag="OO544_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HCR6769823.1"
                     /translation="FSPIYEGKCGMSGGRVEGKVIYETQSTHKLLAAFSQASMIHVKG
                     DVNEETFNEAYMMHTTTSPHYGIVASTETAAAMMKGNAGKRLINGSIERAIKFRKEIK
                     RLRTESDGWFFDVWQPDHIDTTECWPLRSDSTWHGFKNIDNEHMYLDPIKVTLLTPGM
                     EKDGTMSNFGIPASIVAKYLDEHGIVVEKTGPYNLLFLFSIGIDKTKALSLLRALTDF
                     KRAFDLNLRVKNMLPSLYREDPEFYENMRIQELAQNIHKLIVHHNLPDLMYRAFEVLP
                     TMVMTPYAAFQKEL"
BASE COUNT          236 a          219 c          212 g          206 t
ORIGIN      
        1 acttctcacc gatttacgaa ggtaaatgcg gtatgagcgg tggccgtgta gaagggaaag
       61 tgatttacga aacccagtcc actcacaaac tgctggcggc gttctctcag gcttccatga
      121 tccacgttaa aggtgacgta aacgaagaaa cctttaacga agcctacatg atgcacacca
      181 ccacttctcc tcactacggt atcgtggcgt ccactgaaac cgctgcggcg atgatgaaag
      241 gcaatgcagg taaacgtttg atcaacggtt ccattgaacg tgcgatcaaa ttccgtaaag
      301 agatcaaacg tctgagaacg gaatctgatg gctggttctt tgatgtatgg cagccggatc
      361 atatcgatac gactgaatgc tggccgctgc gttctgacag cacctggcac ggcttcaaaa
      421 acatcgataa cgagcacatg tatcttgacc cgatcaaagt caccctgctg actccgggga
      481 tggaaaaaga cggcaccatg agcaactttg gtattccggc cagcatcgtg gcgaaatacc
      541 tcgacgaaca tggcatcgtt gttgagaaaa ccggtccgta taacctgctg ttcctgttca
      601 gcatcggtat cgataagacc aaagcactga gcctgctgcg tgctctgact gacttcaaac
      661 gtgcgttcga cctgaacctg cgtgtgaaaa acatgctgcc gtctctgtat cgtgaagatc
      721 ctgaattcta tgaaaacatg cgtattcagg aactggctca gaatatccac aaactgattg
      781 ttcaccacaa tctgccggat ctgatgtatc gcgcatttga agtgctgccg actatggtaa
      841 tgactccgta tgctgcgttc cagaaagagc tgc
//
LOCUS       DAJTZL010000410          873 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.413, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000410 DAJTZL010000000
VERSION     DAJTZL010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..873
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.413"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            74..454
                     /locus_tag="OO544_004417"
     CDS             74..454
                     /locus_tag="OO544_004417"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HCR6769824.1"
                     /translation="MQKNVTPGRRKGCPNYPPEFKQQLVAASCEPGISISKLALENGI
                     NANLLFKWRQQWREGKLLLPSSESPQLLPVTLDAAAEQPESLAEDPKTLSISCEVTFR
                     HGTLRFNGNVSEKLLTLLIQELKR"
     gene            451..798
                     /gene="tnpB"
                     /locus_tag="OO544_004418"
     CDS             451..798
                     /gene="tnpB"
                     /locus_tag="OO544_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR6769825.1"
                     /translation="MIPLPSGIKIWLVAGITDMRNGFNGLAAKVQTALKDDPMSGHVF
                     IFRGRSGSQVKLLWSTSDGLCLLTKRLERGRFAWPSARDGKVFLTQAQLAMLLEGIDW
                     RQPKRLLTSLTML"
BASE COUNT          217 a          236 c          226 g          194 t
ORIGIN      
        1 cgtctggtca gaatctgacg aattagacaa agtggtgtcc accaaataag tagtgggaac
       61 caaagtgtca gatatgcaga aaaatgtgac tcccggcagg cgaaaaggct gccctaatta
      121 tcctcccgaa tttaaacagc agctcgttgc tgcctcctgt gaacccggga tatccatctc
      181 aaaacttgct cttgaaaatg gcattaacgc caatctgttg ttcaaatggc gacaacaatg
      241 gcgcgaggga aagctgctat taccttcttc agagagcccc cagctacttc ctgtgactct
      301 cgatgcagct gccgaacagc cagaatcgct cgcagaggac ccgaaaaccc tcagtatcag
      361 ctgtgaggta acgttccggc acgggacgct ccgcttcaat ggcaatgtca gcgaaaagct
      421 cctgactctg ctgatacagg aactgaagcg atgatcccgt taccttccgg gatcaaaatt
      481 tggctggttg ccggtatcac cgatatgaga aatggcttca acggcctggc tgcgaaagta
      541 cagacggcgc tgaaagacga tcccatgtcc ggccatgttt tcattttccg gggccgcagc
      601 ggcagtcagg ttaaactgct gtggtccacc agtgacggac tgtgcctcct gaccaaacgg
      661 ctggagcgtg ggcgcttcgc ctggccgtca gcccgtgatg gcaaagtgtt ccttacacag
      721 gcgcagctgg cgatgctgct ggaaggtatc gactggcgac agcctaagcg gctgctgacc
      781 tccctgacca tgctgtaaat ctctttatcc tggttgtcac agaataagcc cggtaaaata
      841 cgggcttatg aacgacatct cttctgacga cat
//
LOCUS       DAJTZL010000411          842 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.414, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000411 DAJTZL010000000
VERSION     DAJTZL010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..842
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.414"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            54..130
                     /locus_tag="OO544_004419"
     tRNA            54..130
                     /locus_tag="OO544_004419"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:88..90,aa:Ile,seq:gat)
     gene            173..248
                     /locus_tag="OO544_004420"
     tRNA            173..248
                     /locus_tag="OO544_004420"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:206..208,aa:Ala,seq:tgc)
     gene            432..>842
                     /locus_tag="OO544_004421"
     rRNA            432..>842
                     /locus_tag="OO544_004421"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          238 a          177 c          232 g          195 t
ORIGIN      
        1 ctttgcagtg ctcacacaga ttgtctgatg aaaatgagca gtaaaacctc tacaggcttg
       61 tagctcaggt ggttagagcg cacccctgat aagggtgagg tcggtggttc aagtccactc
      121 aggcctacca aatttgcatc gcaaatttga agaggtttta actacatgtt atggggctat
      181 agctcagctg ggagagcgcc tgctttgcac gcaggaggtc tgcggttcga tcccgcatag
      241 ctccaccatc tctgtagtga ttaaataaaa aatacttcag agtgtacctg caaaggttca
      301 ctgcgaagtt ttgctcttta aaaatctgga tcaagctgaa aattgaaaca ctgaacaatg
      361 aaagttgttc gtgagtctct caaattttcg caacacgatg atgaatcgca agaaacatct
      421 tcgggttgtg aggttaagcg actaagcgta cacggtggat gccctggcag tcagaggcga
      481 tgaaggacgt gctaatctgc gataagcgtc ggtaaggtga tatgaaccgt tataaccggc
      541 gatttccgaa tggggaaacc cagtgtgatt cgtcacacta tcattaactg aatccatagg
      601 ttaatgaggc gaaccggggg aactgaaaca tctaagtacc ccgaggaaaa gaaatcaacc
      661 gagattcccc cagtagcggc gagcgaacgg ggagcagccc agagcctgaa tcagtgtgtg
      721 tgttagtgga agcgtctgga aaggcgcgcg atacagggtg acagccccgt acacaaaaat
      781 gcacatattg tgagctcgat gagtagggcg ggacacgtgg tatcctgtct gaatatgggg
      841 gg
//
LOCUS       DAJTZL010000412          825 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.415, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000412 DAJTZL010000000
VERSION     DAJTZL010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..825
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.415"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..516)
                     /locus_tag="OO544_004422"
     CDS             complement(<1..516)
                     /locus_tag="OO544_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HCR6769826.1"
                     /translation="MKLNIFTKSMIGMGLVCSALPALAMEAWNNQQGGNKYQVIFDSK
                     IYENAWWGFSTNCPGKAKANDATNPWRLKRTATAAEISQFGNTLSCEKSGSSSSSNSN
                     TPASNTPANGGSATPAQGTVPSNSSVVAWNKQQGGQTWYVVFNGAVYKNAWWVASSNC
                     PGDAKGNDASNP"
BASE COUNT          267 a          173 c          152 g          233 t
ORIGIN      
        1 tgggttgctg gcatcattgc ctttcgcatc acccggacag ttagaagagg ctacccacca
       61 agcattttta tataccgcac cattaaagac gacataccag gtctgaccgc cctgctgttt
      121 attccaggca actacagaag aattagacgg aacagtgccc tgtgctggtg tagccgaacc
      181 gccattagcc ggcgtattgg atgcaggcgt atttgaattt gaagaagatg agctgccgct
      241 cttttcgcag gaaagtgtat tgccaaactg actaatttca gcagctgttg cggtacgctt
      301 taaacgccac gggttagttg catcatttgc tttcgctttt cccgggcaat ttgtagaaaa
      361 accccaccag gcattttcat aaattttgct atcgaaaata acctgatatt tattaccacc
      421 ttgttggtta ttccatgctt ccattgccaa tgctggcaga gcggaacaca ccagccccat
      481 accaatcata gatttagtaa atatatttaa tttcattata atcccttgtg acgtaaaaac
      541 tgcaaaacaa aagtcctacg caggaattat ttacgtttta cgagaaccct tcaatatgaa
      601 ttaaagtgag gcattaaaaa taagaaaaaa gcctgacaaa tgaagaattt taaaaacaga
      661 aacattcata tttaaaatat taaattgaat tgatatttta aatatgaata atttattcgt
      721 tctgacagta cgaataagat atgcagtcaa caaatgcaaa aagggcgccg aagcgccctt
      781 ttcaattcaa aactaattaa cgtgtaatta gcccagaact ttagc
//
LOCUS       DAJTZL010000413          823 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.416, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000413 DAJTZL010000000
VERSION     DAJTZL010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..823
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.416"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..488
                     /locus_tag="OO544_004423"
     CDS             <1..488
                     /locus_tag="OO544_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6769827.1"
                     /translation="NVRRNLTILDMFGPPKTNAGIRTVTLLQPALEALKEQYKLTGHH
                     RKSEITFYHREYGRTEKQKLHFVFMPRVCNEKQKPYYSVSSLGARWNAAVKRAGIRRR
                     NPYHTRHTFACWLLTAGANPAFIASQMGHETAQMVYEIYGMWIDDMNDEQIAMLNARL
                     S"
     gene            540..>823
                     /locus_tag="OO544_004424"
     CDS             540..>823
                     /locus_tag="OO544_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="TtcA; YdaO; catalyzes the thiolation of cytosine 32
                     in specific tRNAs; forms 2-thiocytidine (s(2)C); Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HCR6769828.1"
                     /translation="MQENQQITKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLE"
BASE COUNT          255 a          183 c          197 g          188 t
ORIGIN      
        1 tgaatgtcag aagaaacctg acgatacttg atatgttcgg tcccccaaaa acaaatgccg
       61 ggatccggac ggtaacatta ctgcagccgg ctcttgaagc actgaaggag caatacaaac
      121 tgaccgggca tcatcgcaaa agcgaaatca ctttttatca tcgggagtac ggcagaactg
      181 aaaagcaaaa actgcatttt gttttcatgc ccagggtgtg taacgaaaaa cagaaacctt
      241 attactcggt aagcagtttg ggtgcaagat ggaatgcagc agtaaaacgt gctggtattc
      301 gccgccgtaa tccgtaccat acgcgacata cttttgcctg ctggctgttg acggcaggag
      361 cgaacccggc atttatagcc agccagatgg ggcatgaaac tgcgcaaatg gtgtatgaaa
      421 tttacggtat gtggattgat gacatgaacg acgaacagat agccatgttg aatgcgcggt
      481 tatcgtagtt gcaaagtttg cccccaattt gccccattta gtaccagaga actgaaataa
      541 tgcaagaaaa tcaacaaatt acaaagaaag aacaatacaa cctgaacaaa ttacaaaaac
      601 gtctgcgtcg taacgtgggc gaagccattg ctgacttcaa tatgattgaa gaaggcgatc
      661 gcatcatggt ttgcctctcc gggggtaaag acagctatac catgctggag attctgcgca
      721 atttgcagca aagcgcgcca atcaattttt cgctggtggc tgttaacctc gatcaaaagc
      781 aaccgggctt cccggaacac gttctgcccg agtatcttga aaa
//
LOCUS       DAJTZL010000414          818 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.417, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000414 DAJTZL010000000
VERSION     DAJTZL010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..818
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.417"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..615
                     /locus_tag="OO544_004425"
     CDS             <1..615
                     /locus_tag="OO544_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR6769829.1"
                     /translation="QVKSAATQTFDGIAQNMAAMLTGSEQNWRSFTRSVLSMMTEILL
                     KQAMVGIVGSIGSAIGGAVGGGASASGGTAIQAAAVKFHFATGGFTGTGGKYEPAGIV
                     HRGEFVFTKEATSRIGVGNLYRLMRGYAEGGYVGGAGSPAQMRRAEGINFNQNNHVVI
                     QNDGTNGLPGPQMMKAVYDMARKGARDEIQTQMRDGGLFSGGGR"
     gene            612..>818
                     /locus_tag="OO544_004426"
     CDS             612..>818
                     /locus_tag="OO544_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6769830.1"
                     /translation="MKTFRWKVKPGMDVASAPSVRKVRFGDGYSQRAPAGLNANLKTY
                     SVTLSVPREEAMVLESFLEEHGGWK"
BASE COUNT          179 a          189 c          276 g          174 t
ORIGIN      
        1 caggttaaaa gtgcagccac gcagaccttt gatggtattg cacagaatat ggcggcgatg
       61 ctgaccggca gtgaacagaa ctggcgcagc ttcacccgct ccgtgctgtc catgatgaca
      121 gaaattctgc ttaagcaggc aatggtgggg attgtcggga gtatcggcag cgccattggc
      181 ggggctgttg gtggcggcgc atccgcgtca ggtggtacag ccattcaggc cgctgcggtg
      241 aaattccatt ttgcaaccgg gggatttacg ggaaccggcg gcaaatatga gccagcgggg
      301 attgttcacc gtggtgaatt tgtcttcacg aaggaggcaa ccagccggat tggcgtgggg
      361 aatctctacc ggctgatgcg cggctatgcg gaaggtggtt atgtgggcgg tgccggaagt
      421 ccggcgcaga tgcggcgggc tgaaggcatt aattttaatc agaacaatca cgtggtgatt
      481 cagaacgacg gtacgaatgg tctgccaggt ccacagatga tgaaggccgt gtatgacatg
      541 gcccgcaagg gtgcccgtga tgaaattcag acacagatgc gtgatggtgg cctgttctcc
      601 ggaggtggac gatgaaaacc ttccgctgga aagtgaaacc cggtatggat gtggcttcgg
      661 ccccttctgt aagaaaggtg cgctttggtg atggctattc ccagcgagcg cctgccgggc
      721 tgaatgccaa cctgaaaacg tacagcgtga cgctttctgt cccccgtgag gaggccatgg
      781 tactggagtc gtttctggaa gagcacgggg gctggaaa
//
LOCUS       DAJTZL010000415          815 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.418, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000415 DAJTZL010000000
VERSION     DAJTZL010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..815
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.418"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(127..477)
                     /locus_tag="OO544_004427"
     CDS             complement(127..477)
                     /locus_tag="OO544_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR6769831.1"
                     /translation="MPSLIPRACRKRGCAGTTTDSSGYCDKHRGEGWVQHQRGLSRHQ
                     RGYGSKWTVIRARILKRDKGLCQLCLRVGVVSEAKTVDHIIPKAHGGTDADSNLQSLC
                     WPCHKAKTARERIR"
     gene            complement(520..>815)
                     /locus_tag="OO544_004428"
     CDS             complement(520..>815)
                     /locus_tag="OO544_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HCR6769832.1"
                     /translation="QRDVNALDAKYTKELADAKAENETLRADVAAGRRRLHIKAVCQS
                     VREATTASGVDNATSPRLADTAERDYFTLRERLMTMQMQLEGAQEYIRTQCIK"
BASE COUNT          172 a          233 c          208 g          202 t
ORIGIN      
        1 gccatatgcg gcactcctgg ttaaatttca tttttcgcgg gtataaaaaa acgatggggc
       61 gggcagtccg gaagacgtca ggtcacaggg atttgccccg cccctcccta caagtgagaa
      121 taattatcac cggattcgtt cgcgcgctgt tttcgcttta tggcagggcc agcacagact
      181 ctgcaggttg ctgtctgcgt ctgttccgcc atgcgctttc gggatgatgt ggtcgacggt
      241 tttcgcctcg ctcaccacac cgacacgcag acacaactga cacagacctt tatcgcgctt
      301 cagaatacgg gcacgaatca ccgtccattt tgagccatag ccacgctggt ggcggctcag
      361 tccgcgctgg tgctgcaccc agccttcacc gcgatgttta tcgcagtaac ccgaactgtc
      421 tgtggttgta cctgcacatc cacgcttacg gcaggcacgt gggattagtg atggcataaa
      481 tacctcatac cctgcgaaat gtttaccacg ataaaaaggc tacttaatgc actgagtgcg
      541 gatatactcc tgtgcccctt ccagttgcat ctgcatcgtc atcagccgct ctctgagggt
      601 gaaataatcc cgttcagcgg tgtctgccag tcgggggctg gttgcattat ccacgccgga
      661 ggcggtggtg gcttcacgca ctgactgaca gactgctttg atgtgcaacc gacgacgacc
      721 agcggcaaca tcagcgcgca gagtttcatt ttcagctttc gcatcagcta actccttcgt
      781 gtatttagca tcgagtgcat taacatcacg ctgtc
//
LOCUS       DAJTZL010000416          806 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.419, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000416 DAJTZL010000000
VERSION     DAJTZL010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..806
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.419"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..603
                     /locus_tag="OO544_004429"
     CDS             <1..603
                     /locus_tag="OO544_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR6769833.1"
                     /translation="QVKSAATQTFDGIAQNMAAMLTGSEQNWRSFTRSVLSMMTEILL
                     KQAMVGIVGSIGSAIGGGASASGGTAIQAAAAKFHFAAGGFTGTGGKYEPAGIVHRGE
                     FVFTKEATSRIGVGNLYRLMRGYATGGYVGTPGSMADSRSQASGTLEQNNHVVINNDG
                     TNGQIGPQALKAVYDVARKAAMDVVTGQMRDGGLFSGGGR"
     gene            600..>806
                     /locus_tag="OO544_004430"
     CDS             600..>806
                     /locus_tag="OO544_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6769834.1"
                     /translation="MKTFRWKVKPGMDVASAPSVRKVRFGDGYSQRAPAGLNADLKTY
                     SVTLSVPRWEATALESFLAEHGGWK"
BASE COUNT          172 a          197 c          274 g          163 t
ORIGIN      
        1 caggtaaaaa gtgcagccac gcagaccttt gatggtattg cacagaatat ggcggcgatg
       61 ctgaccggca gtgagcagaa ctggcgcagc ttcacccgtt ccgtgctgtc catgatgaca
      121 gaaattctgc ttaagcaggc aatggtgggg attgtcggga gtatcggcag cgccattggc
      181 ggcggcgcat cagcgtcagg cggtacagcc attcaggcag ctgcggcgaa attccatttt
      241 gcggccggag ggtttacggg aaccggcggc aaatatgagc cagcggggat tgttcaccgt
      301 ggtgaatttg tcttcacgaa ggaggcaacc agccggattg gcgtaggaaa tctttaccgg
      361 ctgatgcgcg gctatgccac cggcggttat gtcggtacac cgggcagcat ggcggacagc
      421 cggtcgcagg cgtccgggac gcttgagcag aataaccatg tggtgattaa caacgacggc
      481 acgaacggtc agatagggcc acaggcactg aaggcggttt atgacgtagc ccgtaaggcg
      541 gcaatggatg ttgtgaccgg gcagatgcgc gatggtggtc tgttctccgg aggtggacga
      601 tgaaaacctt ccgctggaaa gtgaaacccg gtatggatgt ggcttcggcc ccttccgtaa
      661 gaaaggtgcg ctttggtgat ggctattccc agcgagcgcc tgccgggctg aatgccgacc
      721 tgaaaacgta cagcgtgacg ctttctgttc cccgttggga ggccacggcg cttgagtcgt
      781 ttctggctga gcacgggggc tggaaa
//
LOCUS       DAJTZL010000417          802 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.420, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000417 DAJTZL010000000
VERSION     DAJTZL010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..802
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.420"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..123)
                     /locus_tag="OO544_004431"
     CDS             complement(<1..123)
                     /locus_tag="OO544_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HCR6769835.1"
                     /translation="MAFKHYDVVRAAPPSDLAEKLTHKLKEGWQPFGSPVAITPY"
     gene            384..719
                     /gene="iraM"
                     /locus_tag="OO544_004432"
     CDS             384..719
                     /gene="iraM"
                     /locus_tag="OO544_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HCR6769836.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKIKLIIWYQSDAFLPPESIFT
                     LTHTGIMLNNKVLPVTIYNVVPFNKTFWNSIKNSQECPTNTDNVLNECFNNRCTLQIC
                     PYGLKQQSP"
BASE COUNT          292 a          171 c          128 g          211 t
ORIGIN      
        1 ataaggggtt atggccaccg gactaccaaa cggctgccag ccctctttca gtttatgtgt
       61 cagcttttcc gcaagatctg acggcggcgc cgccctgaca acatcatagt gtttaaatgc
      121 catggttctt tccaccatct gaaaaataat tctttaaaat acctgacatg taatacagaa
      181 aaaacacaaa accatacctt aaataaaaac ctgattatca agcagatatg catggataaa
      241 ctacaagacg agatataaac caccctgcat ttaaataaac aataaacaac atcagaaaaa
      301 taattctgct ctatggttta cattcaaaaa tatcatttat acttttcaga acatcaccag
      361 caaggcataa acaaggaaac taaatgaagt ggattgtgat tgatacagtt atccagccat
      421 catgcggaat atctttttca gtcatatgga gtaaaataaa attaataatc tggtatcaat
      481 cggatgcttt cttacctcct gaaagtatat ttacactgac tcacacaggc atcatgctca
      541 ataacaaagt gctacctgta accatttaca acgtagtacc attcaataaa acattctgga
      601 attcaatcaa aaatagccag gaatgcccta caaatacaga taacgtattg aatgaatgct
      661 ttaataaccg ttgcactctg caaatatgtc cttatggact aaaacaacaa agtccataag
      721 gagtttactc acatctgaca aaatcaatat aaacagcccc tccggagagg ggctggagag
      781 tggcgctatg tgccattgca tg
//
LOCUS       DAJTZL010000418          795 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.421, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000418 DAJTZL010000000
VERSION     DAJTZL010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..795
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.421"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..741)
                     /locus_tag="OO544_004433"
     CDS             complement(<1..741)
                     /locus_tag="OO544_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HCR6769837.1"
                     /translation="MRYFILMFTFVCSFVAAQPTIVPQLQQQVTDLTSSLNSQEKKEL
                     THKLESIFNNTQVQLAVLIVPTTKDETIEQYATRVFDNWRLGDAKRNDGILIIVAWSD
                     RTVRIQVGYGLEEKVTDALAGDIIRSNMIPAFKQQKLAQGLELAINALNNQLTSQHQY
                     PTNPSESESASSSDHYYFAIFWVFAVMFFPFWFFHQGSNFCRACKSGVCISAIYLLDL
                     FLFSDKIFSIAVFSFFFTFTIFMVFTCLC"
BASE COUNT          266 a          137 c          161 g          231 t
ORIGIN      
        1 acataaacag gtaaagacca taaatatggt gaaagtaaaa aagaaggaaa atacagcaat
       61 ggaaaaaatt ttatcggaga acaggaataa atctaaaaga tagattgctg aaatacagac
      121 tccactttta catgcgcgac aaaaattact gccttgatga aaaaaccaga aagggaagaa
      181 catcactgca aatacccaaa aaatagcaaa gtaataatga tcactggaag acgctgattc
      241 actttctgaa ggattagtcg gatattggtg ttgtgaagtg agttggttat tcaaagcgtt
      301 tatagctagc tctaatccct gagctaattt ttgttgttta aatgcgggta tcatgttgct
      361 acggatgata tctccagcca gagcatcggt taccttttct tccagcccat aacctacctg
      421 gatgcggaca gtacgatctg accaggcaac aatgatcagt atcccatcat tacgtttggc
      481 atctcctaaa cgccaattgt caaaaactct tgtagcatat tgttcaattg tctcgtcttt
      541 ggttgtggga acaattaata cagcaagttg cacttgggta ttattgaaaa tagattctaa
      601 cttgtgcgtc agttctttct tttcttgtga gtttaagcta ctcgtaagat ccgtaacttg
      661 ttgctgtaat tgtgggacaa tagttggttg ggctgcaaca aaggaacaca cgaaagtgaa
      721 cattagtatg aaatagcgca ttataaattt ctcattaaat ctggataaat aaattgacgg
      781 cggcatcggt aattt
//
LOCUS       DAJTZL010000419          790 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.422, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000419 DAJTZL010000000
VERSION     DAJTZL010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..790
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.422"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>790
                     /locus_tag="OO544_004434"
     CDS             <1..>790
                     /locus_tag="OO544_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SopA family protein"
                     /protein_id="HCR6769838.1"
                     /translation="ECQIRNSNFDNCYLYASHFTRAEFLSAKEISFIKSNLTAVMFDH
                     VRMSTGNFKDCITEQLELTIDYSDIFGNEDLDGYINNIIKMIDTLPDNAMILKSVLAV
                     KLVMQLKILNIVNKNFIENMKKTFSHCPYIKDPIIRSYIHSGEDDKFDNFMRQHRFSK
                     VNFDTQQMIHFINRFNMNKGLIDKNNNFFIQLIDQALRSTDDMIKANAWYLYKEWIRS
                     DDVSPIFIETEEKLRTFNTNKLTRNDNIFILFSSVDDGPVMVVSS"
BASE COUNT          281 a          115 c          134 g          260 t
ORIGIN      
        1 cgaatgtcag attagaaatt caaacttcga taattgttat ctttacgctt cgcacttcac
       61 cagagcagag tttctgtctg ccaaagaaat atcatttatt aaatcgaatt tgacagctgt
      121 tatgtttgat catgtgcgaa tgtcgacagg gaattttaaa gattgcatta cagaacaatt
      181 ggaattaact attgattatt cagatatatt tgggaatgaa gatctcgatg gttatatcaa
      241 taacattata aaaatgattg atacattgcc agataatgca atgatattga aatccgttct
      301 ggcagtaaaa ctggtgatgc aattaaaaat tctcaatatt gttaataaaa actttattga
      361 gaatatgaaa aaaacattta gccattgtcc ttatataaaa gatccaatta tacgtagtta
      421 tatacattct ggggaagatg acaagttcga taattttatg cgtcaacatc gattcagtaa
      481 ggtgaatttc gatacccaac agatgatcca ttttattaac agatttaata tgaataaagg
      541 gctgattgat aaaaataaca atttttttat ccaacttatc gatcaggcct tacgatcaac
      601 ggatgatatg atcaaagcaa atgcctggta tctttataaa gagtggattc gtagtgatga
      661 tgtttcacct atatttatag aaactgaaga aaagttaaga acctttaaca ctaataaatt
      721 aacacgaaac gataatatat ttatcctgtt ctcctctgtc gatgatgggc ctgttatggt
      781 ggtaagctcc
//
LOCUS       DAJTZL010000420          775 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.423, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000420 DAJTZL010000000
VERSION     DAJTZL010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..775
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.423"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..343
                     /locus_tag="OO544_004435"
     CDS             <1..343
                     /locus_tag="OO544_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HCR6769839.1"
                     /translation="ADGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVAHGELGGDFSLV
                     PDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASVLQLHPSLIVIA
                     DKAAAAELALG"
     gene            complement(405..>775)
                     /locus_tag="OO544_004436"
     CDS             complement(405..>775)
                     /locus_tag="OO544_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CbrC family protein"
                     /protein_id="HCR6769840.1"
                     /translation="QDDASIEGVEFEYDEEDEFAGIKNTYPDEMLKELVERTPGYHGW
                     QQEFWLAHCGDFCAFIGYVGWNDIKDRLDEFANLEEDCENFGIRNSDLAKCLQKGGDC
                     QGYLFRCLHCGKLRLWGDFS"
BASE COUNT          210 a          190 c          175 g          200 t
ORIGIN      
        1 tgcagatggt catttttgtg gcaatttgcc gaatacgacc catttccatg agcaaaccgt
       61 ggagttcccg atccagggag agatggttga tattgtggcg catggtgagt tgggcggcga
      121 cttttcgtta gtgcctgaca gctacgtgac gatggggccg aaaagcatca tggcggcgaa
      181 aaacttgctg ataattgtca gtggcgcagg taaagcgcag gcactgaaaa atgtgctgca
      241 aggtccggtt accgaagatg ttccagcttc cgtgttgcag ctgcatcctt cgctgatagt
      301 aatcgctgat aaagccgctg ccgcagaatt agcgctcggt taagctgaag aaataaggcc
      361 ggatgtatga attacgctcc ggcctgttct cattatttaa ataactacga aaaatcaccc
      421 cacagtctca gcttgccgca gtggagacag cggaagagat aaccctgaca atcaccaccc
      481 ttttgcaggc atttagctag atcagaattt ctaataccga aattctcaca atcttcttca
      541 aggttggcaa attcatcgag gcgatctttt atatcattcc agcccacata gccgataaaa
      601 gcacagaaat cgccacaatg cgcaagccag aattcctgtt gccatccatg ataacctggc
      661 gtgcgttcaa ccaactcttt cagcatttca tcaggatatg tgttcttaat accggcaaat
      721 tcgtcctctt catcatactc aaattcaaca ccttctatgc tggcatcatc ctgaa
//
LOCUS       DAJTZL010000421          764 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.424, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000421 DAJTZL010000000
VERSION     DAJTZL010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..764
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.424"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            120..365
                     /gene="tnpA"
                     /locus_tag="OO544_004437"
                     /pseudo
     CDS             120..365
                     /gene="tnpA"
                     /locus_tag="OO544_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612282.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT          244 a          171 c          136 g          213 t
ORIGIN      
        1 atgttcacct ggtctttgtc accaaatacc gacgtcagat ttttgaccat gacgcgacag
       61 aaaaactacg cacttacttt tcaaatgtat gtgctgattt tgaagctgaa ctggttgaaa
      121 tggatggcga accagatcac gtccatttgt taattaacta tcctcccaaa ctggcgatat
      181 ccagtctggt aaacagcctc aaaggcgtat cgggtaggtg actgcgacga gatcgaccag
      241 atattgcagt caggtattac tacaaaggcg ttttgtggag tgttggctat tttgccagta
      301 gctgcggagg tgcgccaata tccgtcatcc gccaatacat tgaacaacag caaacacctg
      361 gttaggtgga aaaccacgcc ttatatccct gccctgaagg acggggtttt acggcgcacc
      421 ggataagcct ggagattatc tacaccagca tggacatcat tgtactatgc cccgtacatg
      481 ccccatcaca tcctcaacat caaagtatta gagccttgca aaccattatg cgtctcagtt
      541 tgtctcatct atcttacgca aaaaaaaata gaactaatat caagcggcgc tatattgcgt
      601 attaaataaa accacctcaa ttacattaat aaatctaaac aaacatcatg caaataattt
      661 ccatggttat ttctaggcta tttaaattaa ataaataaat tatgagtcaa tcataaataa
      721 caacctttaa tataatttaa ctaccaaatt tatattcgtt caca
//
LOCUS       DAJTZL010000422          743 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.425, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000422 DAJTZL010000000
VERSION     DAJTZL010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..743
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.425"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..396)
                     /locus_tag="OO544_004438"
     CDS             complement(<1..396)
                     /locus_tag="OO544_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCR6769841.1"
                     /translation="MLTKRILFSVMLIVSPSVVASEKAHELYDSIYGGKPAPDVINTL
                     HKMAESGDIDAQSLLGWEYYQPRYDTKPDVQEAIKWFELAAKQGDREAPLALGGIYYD
                     GEQVRVDYAKAYALFNQAAQHGVNLAWSRL"
BASE COUNT          233 a          172 c          108 g          230 t
ORIGIN      
        1 caacctggac catgctaaat tcacaccgtg ctgcgcggcc tgattaaata gtgcatacgc
       61 tttggcataa tccacccgca cctgctcacc gtcgtagtag atacccccca gcgctaacgg
      121 agcttctctg tcaccttgtt tagccgctaa ctcgaaccat ttaatcgctt cctgaacatc
      181 aggtttggta tcataacgag gctgataata ttcccagcct aacaggctct gcgcgtcgat
      241 atctcccgat tcagccattt tatgcagtgt atttataaca tcgggagcag gtttaccacc
      301 gtatatgctg tcatacaatt catgcgcttt ctcgctggcc accacgcttg gtgaaactat
      361 cagcattaca gaaaataata ttcttttagt taacatgtat acctttatgt cctcactgtc
      421 atatatttcc ataataatta accaccaaat atccatttaa tgaataaata cagtgccata
      481 aaggtccaag taataagcac acatttagcc tgaaacatta tcattcatgt caccaaatcg
      541 tttatttagt gagataaaac tagcatattc gtaagataaa aaatgcacat ggcaatcaag
      601 aaatgatatt attacttttt tgatgtaaaa ctatttagtt ttaaaattca ttttttcaac
      661 atatagagca ttcattttat gaatgcaaaa aaacatacct gacacacaaa ttactctcct
      721 ctgtaatatt ttctcattaa cac
//
LOCUS       DAJTZL010000423          741 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.426, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000423 DAJTZL010000000
VERSION     DAJTZL010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..741
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.426"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            176..694
                     /locus_tag="OO544_004439"
     CDS             176..694
                     /locus_tag="OO544_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HCR6769842.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
BASE COUNT          214 a          167 c          144 g          216 t
ORIGIN      
        1 cattacccac aatacgatta aattaattaa aaccccacaa ataaattaaa cataagattt
       61 tactcattgt gttttatttc tcacattgat gacgatcgca tgaaaaaatg atattttaat
      121 gagcattctg aaaaaatatc gatgcatttc gagcgaagat ggtggggatc cctgaatgcg
      181 tttttctcac cgactgttct tacttctgat tctcctgctg acgggagcac caatactcgc
      241 tcaggagcct tctgacgttg cgaaaaacgt gcgtatgatg gtttccggta ttgtcagtta
      301 cacccgctgg cctgcgctat cagggccacc aaagttatgt atcttttcat cttcacgttt
      361 tagtaccgcc ctacaagaga atgccgcgac atctttgcct tatctgcctg tcatcattca
      421 cactcaacaa gaggcgatga tttcaggctg caatggtttt tattttggca acgagtcacc
      481 gacattccag atggaattaa ccgaacaata tccgtcaaag gcgttattat taattgccga
      541 acaaaatacc gagtgcatta ttggtagcgc cttttgtctg atcatccaca ataatgacgt
      601 cagatttgcc gtaaacctgg atgccttatc gcgtagcggt gtaaaggtca acccggatgt
      661 cttaatgctc gcacggaaga aaaatgatgg ataacgataa ttctcttaat aagcgcccca
      721 cgtttaaaag agcattacgc a
//
LOCUS       DAJTZL010000424          731 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.427, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000424 DAJTZL010000000
VERSION     DAJTZL010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..731
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.427"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..>731)
                     /locus_tag="OO544_004440"
     CDS             complement(<1..>731)
                     /locus_tag="OO544_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HCR6769843.1"
                     /translation="KAIMVNGPQFGWYAPAYTYGIGLHGAGYDVTGNTPFAYPGLVFG
                     HNGVISWGSTAGFGDDVDIFAERLLAEKPGYYLHNGKWVKMLSREETITVKNGQAETF
                     TVWRTVHGNILQTDQTTQTAYAKSRAWDGKEVASLLAWTHQMKAKNWQEWTQQAAKQA
                     LTINWYYADVNGNIGYVHTGAYPDRQSGHDPRLPVPGTGKWDWKGLLPFEMNPKVYNP
                     LSGYIANWNNSPQKDYPASDLFAFL"
BASE COUNT          194 a          195 c          155 g          187 t
ORIGIN      
        1 caaaaaggca aacagatccg aagcgggata atctttttgg ggagaattgt tccagttagc
       61 aatatatccc gacagggggt tatacacctt agggttcatt tcaaaaggca atagcccttt
      121 ccagtcccat tttcccgtac caggaacggg taatcgcgga tcatggcctg attgacgatc
      181 tggataagca ccagtatgaa cataaccaat attgccgttt acatcagcat agtaccagtt
      241 gatggtcagt gcttgtttcg ctgcctgctg tgtccactcc tgccaatttt tggccttcat
      301 ctgatgagtc caggccagca aagacgccac ctctttacca tcccatgcgc gggatttagc
      361 gtaagccgtt tgtgtcgtct ggtcagtttg gagaatgttg ccatgcaccg tacgccagac
      421 agtaaaggtc tctgcctgac catttttcac cgtaatggtt tcctcacggc ttaacatttt
      481 cacccactta ccattatgca agtagtagcc tggtttctct gccaacagcc gttcagcaaa
      541 aatatcgaca tcatcgccga aacctgccgt tgatccccag gaaatcacac cattatgacc
      601 aaaaaccagc ccaggatagg caaatggtgt attgccagtg acatcataac cagcaccgtg
      661 cagaccaata ccataagtat acgcaggcgc ataccagcca aactgcggac catttaccat
      721 gattgctttc g
//
LOCUS       DAJTZL010000425          726 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.428, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000425 DAJTZL010000000
VERSION     DAJTZL010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..726
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.428"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(158..>726)
                     /locus_tag="OO544_004441"
     CDS             complement(158..>726)
                     /locus_tag="OO544_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6769844.1"
                     /translation="FPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVI
                     AGILLNVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFI
                     AVVLGMIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQI
                     HLLDKYRKVPPEPVHADIPVGAVSDVKA"
BASE COUNT          225 a          179 c          157 g          165 t
ORIGIN      
        1 tggcttgata accggataac aacgtgcgct ttgttgatgc cggatgcggc gtgaattcct
       61 tatccggtct acaaaatttg caaatccaaa gcattacatt aagacgtggc ctgataaaca
      121 tatcgcatca ggccattttc gttagctatt gtccacgtca ggctttcaca tcactcactg
      181 caccgaccgg aatatcagca tgaacaggct cgggcggtac tttgcgatat ttatccagca
      241 gatgaatctg gatcctgcgc agcgtgtcgc cattgaggcg atagaaacgg aagtagagaa
      301 tcagcgttac catgaaaaac agagttggta gagcaatcat aataaactgc atacctaata
      361 gggcttgcgt agactgttca acattcggta catagccaat catccctaac acaaccgcaa
      421 tgaaaaaagc cgcaaaggct gaaccgccct tcaccaccat agtctgcacg gagtaagcga
      481 tactttcaca gcgtacgtgc agtttatatt caccgtaatc aacggtatct gccaccatga
      541 tgacctgtaa tacccagaaa agcgccgttc ccacattcag caaaataccc gcaatcacaa
      601 tgaggacgac gttgtgatag ctcattaatg ccattaacag gagaacacca cagcttaaca
      661 ccggaagaat agatgctccg gcccataaaa tgcgtcggga taatgattta actaagcgtg
      721 ggaaga
//
LOCUS       DAJTZL010000426          723 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.429, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000426 DAJTZL010000000
VERSION     DAJTZL010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..723
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.429"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..325)
                     /locus_tag="OO544_004442"
     CDS             complement(<1..325)
                     /locus_tag="OO544_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HCR6769845.1"
                     /translation="MPPSLRKAVAAAIGGGAIAIASVLITGPGGNDGLEGVRHNPYKD
                     IVGVWTVCYGHTGKDIIPGKTYTEAECKALLNKDLATVARQINPYIKVDIPETTRCAL
                     YSFVYN"
     gene            complement(325..540)
                     /gene="essD"
                     /locus_tag="OO544_004443"
     CDS             complement(325..540)
                     /gene="essD"
                     /locus_tag="OO544_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HCR6769846.1"
                     /translation="MKSMDKLTTGVAYGTSAGSAGYWFLQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGG"
BASE COUNT          196 a          179 c          163 g          185 t
ORIGIN      
        1 cgttgtagac gaacgagtaa agagcgcagc gcgttgtttc cggtatatcg actttgatgt
       61 acgggttaat ttgtctggcg acagtggcaa ggtctttatt caggagggct ttgcactctg
      121 cttcggtata cgttttaccg ggaatgatgt cttttcctgt atgcccgtaa catacagtcc
      181 atacaccaac tatgtctttg taaggattat gtctcacacc ttccagacca tcgttaccac
      241 ctgggccagt gattaacaca gatgctatag caatagcccc gccaccaata gcagcagcaa
      301 cagcttttcg taatgatgga ggcattatcc acctctcgca gccttgcgct tatcttcttt
      361 aatcttgaaa taaaggtttg tcaggtacgt cagcaggcca aataccaggc tacccagcac
      421 tccaattgcc gcccactgtg agggcgtgac tttatctagc agctgtaaaa accagtaccc
      481 ggcactacct gctgaggtgc cataggcgac acccgttgtt aacttatcca tggatttcat
      541 aaccccacct cgcagacaaa gcgggtgtaa attgagggaa tactacgaaa cgtaacagac
      601 tcggagtcag tgaataactc aggtattggg ttatcagcta atatcgagac tcaaaaaatg
      661 gaaaaacccg ctcgacggcg ggtttaagct gtgtgacgaa gtaaccactc ttaacagcat
      721 aac
//
LOCUS       DAJTZL010000427          710 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.430, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000427 DAJTZL010000000
VERSION     DAJTZL010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..710
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.430"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
BASE COUNT          245 a          109 c          147 g          209 t
ORIGIN      
        1 gatcgattca gatgccattt tgcagtagtc tattttataa aaaaattaag taggctaaga
       61 agtaccttag aaatcaattt taaggggtct aaaacgctct gtaacgagct aaactgtata
      121 agtggcagta agatgccttt tagactgaca ctcgcttaga gagcttccta gagcttctgg
      181 gaggattcat tagatatcgt tgtatacaga gacacagtat ctataaaggg tattaagaga
      241 ttcttagaag ttactgtaaa gaaccactaa gataagaaga gagggacaaa gaaccgaagg
      301 aaagtccctt attaagtagg agtcttaata gactgtctta atagataact taaaagtctt
      361 aaaggatatc ttccaagaag agaggaacga agaaccagag gaagtgaccg ttgaccgttt
      421 gaacagactg tttaagtaaa gcctataaag tatctattaa ttaagggcta tgtctcaata
      481 ctagcagttt gtcaatacct ttgtctttag caattcgtgc tatttattaa atatagcact
      541 ttttgttaaa acagagtatg ctttttaacc taaaaccttg tcgcttcgct ctgggtcaca
      601 tgttcttggg ttcttagtga gagttacact atcttctaag gtactcaata aaaagacgag
      661 taacaaccga agaacgagga tagaggtatt gtaataactt agaaggtgtc
//
LOCUS       DAJTZL010000428          694 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.431, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000428 DAJTZL010000000
VERSION     DAJTZL010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..694
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.431"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..173)
                     /locus_tag="OO544_004444"
     CDS             complement(<1..173)
                     /locus_tag="OO544_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
                     /protein_id="HCR6769847.1"
                     /translation="MSGKPAARQGDMTRKGLDIVQGSAGVLIGAPTGVACSVCPGGIT
                     YANPVNPVLGAKVL"
     gene            complement(431..>694)
                     /locus_tag="OO544_004445"
     CDS             complement(431..>694)
                     /locus_tag="OO544_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HCR6769848.1"
                     /translation="AEKFATAIRNHWHVENKLHWRLDVVMNEDDCKIRRGNAAELFSG
                     IRHIAINILTNDKVFKAGLRRKMRKAAMDRNYLASVLAGSGLS"
BASE COUNT          161 a          198 c          121 g          214 t
ORIGIN      
        1 agcaccttcg cacccagcac cgggttcacc gggttagcat aggtaatccc tcccggacac
       61 accgaacagg ccactcccgt cggcgcacct atcagcacac ccgctgaacc ctgcacaata
      121 tccagtccct tgcgggtcat gtcgccctgc cgcgccgccg gttttccgct catggtttat
      181 tcctcttcat ctgaattgat ttacattagg ttggttattt tctcataccc acaactatat
      241 tttctctctg aatttattac gtgcaagaca atcgcagcac tctaactaac ttcgatgcaa
      301 tttgatgttc agaaaaatcc ctcattagat tattatcatg aaatacagct aatcatcttt
      361 agcttatata tttctcaatg tattcttttg aaaggatccg tgccctttca aactcttcag
      421 cagggcaaga ttacgaaagc ccgctccccg caaggactga cgccagatag tttctgtcca
      481 tggctgcttt tcgcatctta cgtcttaacc ctgccttgaa taccttatca ttcgtcaaaa
      541 tattaatagc aatgtgccgt atccctgaaa ataattctgc tgcatttcct cttcttattt
      601 tgcagtcgtc ttcattcatt accacgtcca gacgccagtg cagcttattc tccacgtgcc
      661 agtggtttcg gattgctgtg gcgaactttt ctgc
//
LOCUS       DAJTZL010000429          692 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.432, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000429 DAJTZL010000000
VERSION     DAJTZL010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..692
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.432"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>692
                     /gene="tuf"
                     /locus_tag="OO544_004446"
     CDS             <1..>692
                     /gene="tuf"
                     /locus_tag="OO544_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR6769849.1"
                     /translation="VDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHILLG
                     RQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQYDFPGDDTPIVRGSALKALEG
                     DAEWEAKILELAGFLDSYIPEPERAIDKPFLLPIEDVFSISGRGTVVTGRVERGIIKV
                     GEEVEIVGIKETQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPG
                     TIKPHTKFESEV"
BASE COUNT          161 a          166 c          205 g          160 t
ORIGIN      
        1 gtagactgcc cggggcacgc cgactatgtt aaaaacatga tcaccggtgc tgctcagatg
       61 gacggcgcga tcctggtagt tgctgcgact gacggcccga tgccgcagac tcgtgagcac
      121 atcctgctgg gtcgtcaggt aggcgttccg tacatcatcg tgttcctgaa caaatgcgac
      181 atggttgatg acgaagagct gctggaactg gttgaaatgg aagttcgtga acttctgtct
      241 cagtacgact tcccgggcga cgacactccg atcgttcgtg gttctgctct gaaagcgctg
      301 gaaggcgacg cagagtggga agcgaaaatc ctggaactgg ctggcttcct ggattcttac
      361 attccggaac cagagcgtgc gattgacaag ccgttcctgc tgccgatcga agacgtattc
      421 tccatctccg gtcgtggtac cgttgttacc ggtcgtgtag aacgcggtat catcaaagtt
      481 ggtgaagaag ttgaaatcgt tggtatcaaa gagactcaga agtctacctg tactggcgtt
      541 gaaatgttcc gcaaactgct ggacgaaggc cgtgctggtg agaacgtagg tgttctgctg
      601 cgtggtatca aacgtgaaga aatcgaacgt ggtcaggtac tggctaagcc gggcaccatc
      661 aagccgcaca ccaagttcga atctgaagtg ta
//
LOCUS       DAJTZL010000430          690 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.433, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000430 DAJTZL010000000
VERSION     DAJTZL010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..690
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.433"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            132..>690
                     /locus_tag="OO544_004447"
     CDS             132..>690
                     /locus_tag="OO544_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000878026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISEc11 family transposase"
                     /protein_id="HCR6769850.1"
                     /translation="MKYTPVGVDIAKHVIQIHFINEHTGEVVDKQLRRQDFLTFFGNR
                     EPCLIGMEACGGSQHWARELTKLGHKVRLLQARFVKAFVMGNKNDVMDARAIWMAVQQ
                     PGKEIAVKTEEQQSVLVLHRTRMQLVKFRTAQINALHGTLLEFGETIHKGRAAMEREF
                     PEALERMKERLPPYLIMVLENQYNRL"
BASE COUNT          193 a          157 c          192 g          148 t
ORIGIN      
        1 cacacaatta aacacggagc agtatgatgg ttaaaagggt gaaactggca ggtgtggagc
       61 ctcgcagatt tccggcatca tagtgcccaa tacgggatgc ataaagctcc ctcagactgg
      121 agactccaca aatgaaatat acaccggttg gcgttgatat cgcaaaacat gtcattcaga
      181 ttcacttcat caatgagcac acaggtgaag tggttgataa acagttgcgt agacaggatt
      241 ttctgacgtt cttcggcaac cgtgagccat gcctgattgg tatggaggcc tgtggaggtt
      301 ctcagcactg ggcacgggaa ctgacaaaac ttggtcataa agtccggttg ttgcaggccc
      361 gcttcgttaa ggcattcgtc atgggcaata agaatgatgt gatggatgcc cgggctatct
      421 ggatggcggt tcagcagccg ggtaaagaaa tcgccgtaaa aacagaagaa cagcagtcgg
      481 tactggttct gcaccgtacc cgcatgcaac tggtgaagtt ccggaccgca caaattaatg
      541 ccctgcacgg gacgttactg gagtttggtg aaaccatcca caaaggccgg gcagcgatgg
      601 agcgggagtt ccccgaagca ctggaacgga tgaaagagag actgccaccg tatctcatta
      661 tggttctgga aaaccagtac aaccgactga
//
LOCUS       DAJTZL010000431          683 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.434, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000431 DAJTZL010000000
VERSION     DAJTZL010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..683
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.434"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..560
                     /locus_tag="OO544_004448"
     CDS             <1..560
                     /locus_tag="OO544_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HCR6769851.1"
                     /translation="RFSQLESALNTQKNSIPALEKEVKALDKQMVAAQKAADAYWGKD
                     ANGKQMTREEAFKKIHQQRDEFNKQNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVP
                     IQQKRDFDINEQRRQTFLQSQKLSRKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMK
                     IDDINQANERWKKDTEQLRRNGVIK"
BASE COUNT          244 a          121 c          165 g          153 t
ORIGIN      
        1 atcgctttag tcagcttgaa tctgcgttga atacccagaa gaactcgatt cctgcgctgg
       61 aaaaggaagt gaaagcgctt gataagcaaa tggtggcggc gcagaaagca gcggatgcat
      121 attggggaaa agatgcaaac ggtaagcaaa tgacccgcga agaagccttt aaaaaaattc
      181 accaacagcg tgatgagttt aataaacaga acgatagcga ggcgttcgcg gttaaatatg
      241 acaaagaggt ttatcaaccg gcgattgcgg catgtcataa acagagtgaa gagtgttatg
      301 aagtgccgat tcagcagaaa cgagatttcg atatcaacga acaacggcga cagacttttc
      361 tgcaatcaca aaaactcagc cgtaaattgc aggatgactg ggtaacgctc gaaaaagggc
      421 aatatccgtt aacgatgaaa gtttcagaaa taaacagtaa aaaagtcgcg attttgatga
      481 aaattgacga tatcaaccaa gctaatgaac gctggaaaaa agataccgaa caactgcggc
      541 gcaatggggt gattaagtaa ctcatgttaa gtgcctttgc cggataatat aaaaagttat
      601 ccggcaaatt attttttaca ttagccctag tagctgtgga atgaacaagg atatttgcgg
      661 aatgtaagtg atcatcatca acg
//
LOCUS       DAJTZL010000432          677 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.435, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000432 DAJTZL010000000
VERSION     DAJTZL010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..677
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.435"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..361
                     /locus_tag="OO544_004449"
                     /pseudo
     CDS             1..361
                     /locus_tag="OO544_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
BASE COUNT          194 a          170 c          171 g          142 t
ORIGIN      
        1 gcagatgcaa agccagatgg acccggtata cacgccggcg cgaaaaattc acctgtacca
       61 ctgcgaccat cgcggcctgc cgctggccct tatcagcacg gaagggacaa cagcgtggtg
      121 cgcagaatat gatgaatggg gcaacctgct gagtgatgag aacccgcatc atctgcagca
      181 gctcattcgc ctgccggggc agcagtatga tgaggagtcc ggcctgtatt acaaccgcca
      241 ccgctattat gacccgctgc aggggcggta tatcactcag gatccgattg gactgaaggg
      301 gggatggaat ttttatcagt atccgttgaa tccggttcag tatatagatt caatggggct
      361 ggcatctaaa tatggacact taaataatgg cggatatgga gcgaaaccca gcaaaccgcc
      421 tacgcccgat ccaagtaagt tgccagacat agcgaaacaa ttaagactgc catatcctat
      481 tgaccaggcc agtagtgcgc ctaatgtttt caaaacattc ttcagagcat taagccctta
      541 cgattacaca ctgtattgca ggaagtgggt aaaacccaat ctgacttgta tgccgcagga
      601 tgatccccag tatccaggga tggagacaaa gacagcaagt gattacctgc cacagacaaa
      661 ttggtcaaca actcaat
//
LOCUS       DAJTZL010000433          672 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.436, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000433 DAJTZL010000000
VERSION     DAJTZL010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..672
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.436"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            505..>672
                     /locus_tag="OO544_004450"
     CDS             505..>672
                     /locus_tag="OO544_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HCR6769852.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVID"
BASE COUNT          198 a          119 c          149 g          206 t
ORIGIN      
        1 tatcaataga ggtaaagtcg caaccccaaa tcgtaaagga aaccgtagca cgtcgtatgc
       61 aagaacgtgc cacggctggc tgatggatgt tcgatagcgc gagtttgaat gaaaatcagc
      121 cggagatgat tttacataat tgctacggaa ttattcaata caggaattgc ttgcgtctgc
      181 atggattgac ctgaaatatt cccgaaaatt tctctaaaaa actcgaaaaa aatggtaact
      241 ggttgaatgt attaatatgc aatggtacgt gtcagggatt aaaagatgaa cgtaaattta
      301 tttaacgcat taattttaaa gggttttatt gtttgttgac gaaaacagga atcgtgttcg
      361 gtctcttttt atctgttaaa agccagaagc atttccttcg ctgactttat agtcaaccat
      421 aacacacact ctactgtctg agtccaacgt tttttaacat tcttgttaaa attatgtgat
      481 ctttagcgcg ggaggaaaat attgatgaaa cagcctgcgc ccgtttatca gagaattgcg
      541 ggtcatcaat ggcgacatat ctggctttct ggcgatatac acggttgtct tgagcagttg
      601 cgccgcaaat tatggcattg tcgttttgat ccgtggcgag atttacttat ctcagtggga
      661 gacgttatcg at
//
LOCUS       DAJTZL010000434          658 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.437, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000434 DAJTZL010000000
VERSION     DAJTZL010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..658
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.437"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..373
                     /locus_tag="OO544_004451"
     CDS             <1..373
                     /locus_tag="OO544_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6769853.1"
                     /translation="GTVSQPGRNVRAKSGLNRSILDQGWYEMHRQLEYKQLWRGGQVL
                     AVPPAYTSQRCAYCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACG
                     EMVQSGRPLKQEPTEMIQATA"
     gene            complement(413..>658)
                     /locus_tag="OO544_004452"
     CDS             complement(413..>658)
                     /locus_tag="OO544_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate/H(+) symporter BenE family transporter"
                     /protein_id="HCR6769854.1"
                     /translation="AALPVSWIQMLAGLALLSTIGGSLYQALHNERERERDAAVVAFL
                     VTASGLTLVGIGSAFWGLIAGGVCYVVLNLIADRNRY"
BASE COUNT          165 a          188 c          179 g          126 t
ORIGIN      
        1 gggtacggtc agccagccgg ggcgcaatgt ccgggcaaaa tcaggtttaa accgttcgat
       61 actggatcag ggctggtatg aaatgcaccg ccagcttgag tacaagcagc tctggcgtgg
      121 cggtcaggtg cttgctgttc cgccagcgta tacaagccag cgttgcgcgt actgtggtca
      181 tacagcgaaa gagaaccgcc tgtcacaaag taaattcaga tgccaggtat gtggatatac
      241 agcgaacgcc gatgtaaatg gtgctcgtaa cattttagcg gcggggcacg ccgttcttgc
      301 ctgtggagag atggtgcagt caggccgccc gttgaagcag gaacccaccg aaatgattca
      361 ggcgacagcc tgaacgtagc agggatccac gtccttcagg gcgtggagga tgtcaatatc
      421 ggtttctgtc agcgattaaa ttcaacacca cgtaacaaac gcctccggca attaatcccc
      481 agaacgcaga accaatcccg accagcgtca atccacttgc cgttaccaga aatgccacca
      541 ccgccgcgtc gcgctcacgc tcacgctcat tatgcagcgc ctgatacaaa ctgccgccaa
      601 tggtactcaa cagcgccaga cctgccagca tctggatcca acttacgggc agggcagc
//
LOCUS       DAJTZL010000435          649 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.438, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000435 DAJTZL010000000
VERSION     DAJTZL010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..649
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.438"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..196
                     /locus_tag="OO544_004453"
     CDS             <1..196
                     /locus_tag="OO544_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HCR6769855.1"
                     /translation="DVDLHQVLWSRSRLGERQKGQGITGADHFWFGHTPLRHRVDIGN
                     LHYIDTGAVFGGELTLVQLQ"
     gene            complement(197..388)
                     /locus_tag="OO544_004454"
     CDS             complement(197..388)
                     /locus_tag="OO544_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HCR6769856.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(493..>649)
                     /locus_tag="OO544_004455"
     CDS             complement(493..>649)
                     /locus_tag="OO544_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HCR6769857.1"
                     /translation="TIPCKSDPDLCMQLDAWDAETSIPALLNGEHSVLYRTRYDQQSD
                     AWIMRLA"
BASE COUNT          182 a          147 c          162 g          158 t
ORIGIN      
        1 ggacgttgat ttgcatcagg tcttgtggag ccgctcgcga ttaggtgaac gccaaaaagg
       61 gcagggaatt acaggtgctg atcatttctg gtttggtcat acaccgttgc gacatcgcgt
      121 ggatattggc aacctgcatt atattgatac cggtgctgtc tttgggggcg aactgactct
      181 tgtgcaattg caataattaa aaatcaccgt actcctgtgc aggtcgccag aaaccatcta
      241 taaaatcctc aatcggaaaa caaccgccat ggcggatccg ttgatcgctc atagaataaa
      301 gacactgctg ttccgtgttg tagacatcca caacaatatc ttcacaaccg ccatccaggt
      361 agcaaacaaa aagtaccagc gcgaacattt catccccgaa gtgtggtgcc gtaccgttaa
      421 gtttaggaga gattttacaa cgggggaata accaggacaa ataacccgcc atcccgacgg
      481 gttctttttg gatcaggcaa gacgcataat ccaggcatca gattgttgat cgtaacgggt
      541 acggtaaagg acagagtgtt cgccatttag cagggcaggg atactggttt cagcatccca
      601 ggcatcgagt tgcatacata aatcggggtc agatttacaa ggaatggtt
//
LOCUS       DAJTZL010000436          641 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.439, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000436 DAJTZL010000000
VERSION     DAJTZL010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..641
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.439"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>641
                     /gene="fliD"
                     /locus_tag="OO544_004456"
     CDS             <1..>641
                     /gene="fliD"
                     /locus_tag="OO544_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HCR6769858.1"
                     /translation="VSTEAGAAPGIYTISVTQLAQAQSLRTDSPTIIASTKDALGDES
                     SDTRTIKITQDGRKEPLEIKLNKDQTSLDEISKAINDADSGISASIVKVKDGNYQLVL
                     TASEGLANKMTISVEGDSKLNDLLAYDSKTNTGNMKELVNAQNAQLNVNGIDIERSSN
                     KITDAPQGVTLDLTKKVTDVRVTVTKSNDKATEAIKGWVDSYNSLIDTFNTLT"
BASE COUNT          217 a          146 c          139 g          139 t
ORIGIN      
        1 aagtttccac cgaagcaggt gctgcccccg gtatctatac catcagcgta acccaactgg
       61 cgcaggcgca atctttacga actgattccc cgacaatcat tgcatcaaca aaagatgcac
      121 tgggtgatga gtcatcagat acacgcacaa taaaaattac ccaagacggt cgtaaagagc
      181 cgctggaaat caagctcaat aaagatcaga cgtcattgga tgagatcagc aaagccatta
      241 acgatgccga tagtggaatt tccgctagta tcgttaaagt caaagatggt aactatcaat
      301 tggtattgac cgccagtgaa ggtctggcca ataaaatgac catatctgtt gagggtgaca
      361 gtaaattaaa tgatctgctg gcttatgaca gcaagaccaa caccgggaat atgaaggaac
      421 tggttaacgc gcaaaatgca cagctaaacg ttaacggtat cgatattgaa cgttcaagta
      481 acaaaattac cgatgctcct cagggagtga ctctcgattt gaccaaaaaa gtcactgacg
      541 tccgggtcac ggtgacaaaa agcaacgata aagcaaccga agcaattaaa ggctgggtgg
      601 attcatataa ctccctcatt gacaccttta acaccctgac a
//
LOCUS       DAJTZL010000437          615 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.440, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000437 DAJTZL010000000
VERSION     DAJTZL010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..615
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.440"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..548
                     /locus_tag="OO544_004457"
     CDS             <1..548
                     /locus_tag="OO544_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HCR6769859.1"
                     /translation="NQGRLNKAFEKKFPLTELNNPAHDSSAMSEKSHGREEIRLHIVC
                     DVPDELIDFTFEWKGLKKLCVAVSFRSIIAEQKKEPEMMVRYYISSADLTAEKFATAI
                     RNHWHVENNLHWRLDVVMNEDDCKIRRGNAAELFSGIRHIAINVLTNDKVFKAGLRRK
                     MRKAAMDRNYLASVLAGSGLS"
BASE COUNT          199 a          106 c          149 g          161 t
ORIGIN      
        1 gaaaccaggg gcggctaaat aaagcctttg agaaaaaatt tccgctgaca gaattaaata
       61 atccagcgca tgacagttcc gcaatgagtg aaaagagtca cggcagagaa gaaatccgtc
      121 ttcatattgt ttgcgatgtc cctgatgaac ttattgattt cacgtttgaa tggaaagggc
      181 tgaaaaaatt atgcgtggca gtctcctttc gctcaataat agcagagcaa aagaaagagc
      241 ccgaaatgat ggtcagatat tatatcagtt ctgctgattt aaccgctgag aagtttgcga
      301 cagcgattcg aaatcactgg cacgtggaga ataatctgca ctggcgtctg gacgtggtaa
      361 tgaatgaaga cgactgcaaa ataagaagag gaaatgcagc agaattattt tcagggatac
      421 ggcacattgc tattaatgtt ttgacgaatg ataaggtatt caaggcaggg ttaagacgta
      481 agatgcgaaa agcagccatg gacagaaact atctcgcgtc agtccttgcg gggagcggac
      541 tttcgtaatc ttgccctgag gagttcactt tttgtactaa ataattcgca ttttatgttt
      601 aaaaattgag atatt
//
LOCUS       DAJTZL010000438          599 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.441, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000438 DAJTZL010000000
VERSION     DAJTZL010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..599
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.441"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>599
                     /locus_tag="OO544_004458"
     CDS             <1..>599
                     /locus_tag="OO544_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR6769860.1"
                     /translation="GQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYEFPQPLHDALD
                     KFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHKFGLAPLGCGW
                     VIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRLGREGYTKVQN
                     ASYQVAAYLADEIAKLGPYEFICAGRPDEGIPAVCFKLK"
BASE COUNT          121 a          171 c          171 g          136 t
ORIGIN      
        1 ggtcagttgt ttatggaccc gaaacgcatg attgaagcct gcgacgaaaa taccatcggc
       61 gtggtgccga ctttcggcgt gacctacacc ggtaactatg agttcccgca accgctgcac
      121 gatgcactgg ataaattcca ggccgacacc ggtatcgaca tcgacatgca catcgacgcc
      181 gccagcggtg gcttcctggc accgttcgtc gccccggata tcgtctggga cttccgcctg
      241 ccgcgtgtga aatcgatcag tgcttcaggc cacaaattcg gtctggctcc gctgggctgc
      301 ggctgggtta tctggcgtga cgaagaagcg ctgccgcagg aactggtgtt caacgttgac
      361 tacctcggtg gtcagattgg tacttttgcc atcaacttct cccgcccggc gggtcaggtg
      421 attgcacagt actatgaatt cctgcgcctt ggtcgtgaag gctataccaa agtacagaat
      481 gcttcctacc aggtcgctgc ctatctggcg gatgagatcg ccaaactggg gccgtatgag
      541 ttcatctgtg ccggtcgccc ggacgaaggc atcccggcgg tttgcttcaa actgaaaga
//
LOCUS       DAJTZL010000439          589 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.442, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000439 DAJTZL010000000
VERSION     DAJTZL010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..589
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.442"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            289..>589
                     /gene="mcbR"
                     /locus_tag="OO544_004459"
     CDS             289..>589
                     /gene="mcbR"
                     /locus_tag="OO544_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HCR6769861.1"
                     /translation="MPGTGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVGKRQLDEINRIRYELELMAVALA"
BASE COUNT          171 a          121 c          148 g          149 t
ORIGIN      
        1 cggtttagag aatgcgccac agacgtttat cggcctgctg aagggtaaaa acttcggcaa
       61 agtggtgatc cgcgtcgcgg gtgatgatta aagtttcaac ggcggcgtaa gccgccataa
      121 aaatccaggc aaaaagctta ttttcagctt taattaacta accacgtcat tgttctgctg
      181 ctaataacaa caaaggtggg tggtttatga ttattcactt taatacacca ggtgaactcc
      241 ttctgccgtg caggcagggt tggacagaaa acacgcgaag gaattaaaat gcctggaacg
      301 ggaaaaatga aacatgtcag tttgactctg caggttgaga acgacctgaa acatcagctt
      361 agtattggcg cattaaaacc tggcgcacgc ctgattacta aaaatctggc ggagcaatta
      421 ggtatgagta ttacacctgt gcgtgaagca ttattacgtc tggtttcggt gaatgcgctt
      481 tctgtcgcac ctgcacaagc atttacagtt ccggaagtgg ggaaacgtca attagacgaa
      541 atcaatcgga tccgctacga actggaatta atggcagttg ctctggctg
//
LOCUS       DAJTZL010000440          578 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.443, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000440 DAJTZL010000000
VERSION     DAJTZL010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..578
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.443"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
BASE COUNT          157 a          113 c           92 g          216 t
ORIGIN      
        1 tgttatttat acttatcggg tatcagtttt gataggggta tcagacttga taggggtaac
       61 agaattctgt gggggtagtt cactatgacc tacccctagt tcactgtgag ctaccgttac
      121 atccaatcat cttccgatgt aacgtatcgt tactaccgat gtgtaatatc gttacattca
      181 ataattagtc tatgtaatat atcgttacta ccaactttct tacagagagc atttcagata
      241 aatgaatgta acctctcgtt acatccattt gtcacatagc actctgtgta atgtctttct
      301 atgtgtgtgc cataatcccc ctatattccc tgtgtaaatc atagaataat gctctctatt
      361 ccctgtgtaa ttccctacta aaaaatccaa tgaaaagtag cttaaaagtt caattcttac
      421 tttcttcact tttacatcac tttcgcaaag ccttgtgtaa tgcggcttac agagcaccat
      481 cagaaattgt tggctaaaaa ttgttctgtt tgtttctgaa cgagtttctc gtttgtgcaa
      541 attttaaaat ttattttgtt tttatagcct tatttcgt
//
LOCUS       DAJTZL010000441          575 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.444, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000441 DAJTZL010000000
VERSION     DAJTZL010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..575
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.444"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>575
                     /locus_tag="OO544_004460"
     CDS             <1..>575
                     /locus_tag="OO544_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6769862.1"
                     /translation="IAQQKAKTEKQNQRAQAAAARAQARQQQQIAARKEKAELKLLDV
                     SSSISAMHRLSVAEQYKAIAQAREIAKQYEQGAISLARMNSQMKRLQQQQRKINGNRK
                     AQFAPVKGGSGNSATMGALLFGGATAAAGVMAVSKTSEFMANSFANAETEGELIQRAR
                     LGGVDYNLLNNLERWAYDNGIDSLQGDPGRR"
BASE COUNT          192 a          122 c          147 g          114 t
ORIGIN      
        1 attgctcaac agaaagctaa aacagaaaag cagaatcaaa gagcacaagc tgccgcagca
       61 agagcacagg caaggcaaca gcaacaaata gcagcacgta aagagaaagc agaattaaaa
      121 ctccttgatg tatcaagctc tatcagtgca atgcaccgtc tctctgtagc ggaacaatac
      181 aaagctattg cacaggctag agagatagca aaacaatatg agcaaggggc tatcagcctg
      241 gcgcgtatga atagtcagat gaaacgccta caacagcaac aacgtaagat taatggcaac
      301 agaaaagctc aatttgcccc cgttaaggga ggatcaggga attccgccac gatgggcgct
      361 ctgttatttg gaggcgctac ggctgctgct ggcgtgatgg ctgttagtaa aacctctgaa
      421 tttatggcta acagctttgc taatgctgaa acagaagggg agctaatcca acgtgcaagg
      481 cttggtggag ttgattacaa cttacttaat aatcttgagc gttgggcgta tgacaacggg
      541 atcgatagcc tccagggtga tccgggacgc agaaa
//
LOCUS       DAJTZL010000442          570 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.445, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000442 DAJTZL010000000
VERSION     DAJTZL010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..570
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.445"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..439
                     /locus_tag="OO544_004461"
     CDS             <1..439
                     /locus_tag="OO544_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000878026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS110-like element ISEc11 family transposase"
                     /protein_id="HCR6769863.1"
                     /translation="DIEKQLTSVARQNETCKRLLDIPGVGPLIATAAVATMGEASAFK
                     SGREFAAYVGLVPKQTGSGGKVRLLGISKRGDTYLRTLFIHGARAVALVAKEPGPWIT
                     ELKKRRPASVAIVAMANKLARTVWAITAHDRKYDRNHVSIRPY"
BASE COUNT          168 a          124 c          159 g          119 t
ORIGIN      
        1 ggatattgaa aaacagctta ccagcgtggc gaggcagaat gaaacctgta agcggttgct
       61 ggatattcct ggcgttggac cacttattgc gacggcagcg gtggccacca tgggggaagc
      121 atcagcgttt aaatcggggc gagagttcgc cgcatatgtt ggtctggttc caaaacaaac
      181 tggctccgga gggaaagtac gtctgctggg gataagcaaa cgtggtgaca cttatctcag
      241 gacattattt atccacggtg caagagcggt ggcattagta gctaaagagc ctggcccgtg
      301 gataaccgaa ctgaaaaaac gtcgtccagc cagtgtggca atcgtcgcca tggcaaacaa
      361 gctggcacga acagtatggg cgataaccgc ccatgaccgt aagtatgaca ggaaccacgt
      421 cagtatcaga ccatattaat cgctgatacc attaaacaat gaactcttaa caaaagggtg
      481 aatgctgaaa ggttgctatg gcggccagag tgatgacaaa gacaggtaag accgtgactc
      541 actaaacctg aacagtattt tgggcttgaa
//
LOCUS       DAJTZL010000443          557 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.446, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000443 DAJTZL010000000
VERSION     DAJTZL010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..557
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.446"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>557
                     /locus_tag="OO544_004462"
     CDS             <1..>557
                     /locus_tag="OO544_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR6769864.1"
                     /translation="DENTIGVVPTFGVTYTGNYEFPQPLHDALDKFQADTGIDIDMHI
                     DAASGGFLAPFVAPDIVWDFRLPRVKSISASGHKFGLAPLGCGWVIWRDEEALPQELV
                     FNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAAYLADEIA
                     KLGPYEFICTGRPDEGIPAVCFKLK"
BASE COUNT          115 a          160 c          157 g          125 t
ORIGIN      
        1 gacgaaaaca ccatcggcgt ggtgccgact ttcggcgtga cctacaccgg taactatgag
       61 ttcccacaac cgctgcacga tgcgctggat aaattccagg ccgacaccgg tatcgacatc
      121 gacatgcaca tcgacgctgc cagcggtggc ttcctggcac cgttcgtcgc cccggatatc
      181 gtctgggact tccgcctgcc gcgtgtgaaa tcgatcagtg cttcaggcca taaattcggt
      241 ctggctccgc tgggctgcgg ctgggttatc tggcgtgacg aagaagcgct gccgcaggaa
      301 ctggtgttca acgttgacta cctgggtggt caaattggta cttttgccat caacttctcc
      361 cgcccggcgg gtcaggtaat tgcacagtac tatgaattcc tgcgcctcgg tcgtgaaggc
      421 tataccaaag tacagaacgc ctcttaccag gttgccgctt atctggcgga tgaaatcgcc
      481 aaactggggc cgtatgagtt catctgtacg ggtcgcccgg acgaaggcat cccggcggtt
      541 tgcttcaaac tgaaaga
//
LOCUS       DAJTZL010000444          551 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.447, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000444 DAJTZL010000000
VERSION     DAJTZL010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..551
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.447"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..551
                     /locus_tag="OO544_004463"
                     /pseudo
     CDS             1..551
                     /locus_tag="OO544_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS2 family transposase"
BASE COUNT          135 a          136 c          163 g          117 t
ORIGIN      
        1 cctgcctctg aacttgctgc cgccatgaag cagattaaag aactccagcg cctgctcggc
       61 aagaaaacga tggaaaatga actcctcaaa gaagccgttg aatatggacg ggcaaaaaag
      121 tggatagcgc acgcgccctt attgcccggg gatggggagt aagcttagtc agccgttgtc
      181 tccgggtgtc gcgtgcgcag ttgcacgtca ttctcagacg aaccgatgac tggatggatg
      241 gccgccgcag tcgtcacact gatgatacgg atgtgcttct ccgtatacac catgttatcg
      301 gagagctgcc cacgtatggt tatcgtcggg tatgggcgct gcttcgcaga caggcagaac
      361 ttgatggtat gcctgcgatc aatgccaaac gtgtttaccg gatcatgcgc cagaatgcgc
      421 tgttgcttga gcgaaaacct gctgtaccgc catcgaaacg ggcacataca ggcagagtgg
      481 ccgtgaaaga aagcaatcag cgatggtgct ctgacgggtt cgagttctgc tgtgataacg
      541 gagagagact g
//
LOCUS       DAJTZL010000445          550 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.448, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000445 DAJTZL010000000
VERSION     DAJTZL010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..550
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.448"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>550
                     /gene="feaR"
                     /locus_tag="OO544_004464"
     CDS             <1..>550
                     /gene="feaR"
                     /locus_tag="OO544_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator FeaR"
                     /protein_id="HCR6769865.1"
                     /translation="QEVKGSDDAWFYTVFQLSGQAIMEQDERQVQIGAGDITLLDASR
                     PCSLYWQESSKQISLLLPRTLLEQYFPHQKPVCAERLDADLPMVQLSHRLLQESMNNP
                     ALSETESEAALQAMVCLLRPVLHQRESVQPRRERQFQKVVTLIDDNIREEILRPEWIA
                     GETGMSVRSLYRMFADKGLVVA"
BASE COUNT          139 a          125 c          146 g          140 t
ORIGIN      
        1 ggcaggaagt aaaaggcagc gacgatgcct ggttttacac cgtttttcag cttagtggtc
       61 aggcaataat ggagcaggat gagcgtcagg tgcagattgg cgctggcgat attacgttac
      121 tcgatgcctc acgcccctgt tcgctttact ggcaggagtc ttctaaacag atttcattac
      181 ttttgccacg cactctgctg gaacaatatt ttccccatca aaaacctgtc tgcgcagaaa
      241 gactggacgc tgacttaccc atggtgcaac tcagtcatcg cctgttacag gagagcatga
      301 ataatccggc actttctgaa acagaaagtg aagctgcgct acaggcgatg gtgtgtctgc
      361 tgcgcccggt acttcatcag cgggaatctg ttcaacctcg ccgtgaacgt cagtttcaaa
      421 aagtggttac gttgatagac gataatattc gcgaagagat attacgcccg gagtggatag
      481 ccggagagac aggtatgtca gtacgtagtt tgtaccgaat gtttgccgat aaaggtttgg
      541 tagtcgcgca
//
LOCUS       DAJTZL010000446          546 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.449, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000446 DAJTZL010000000
VERSION     DAJTZL010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..546
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.449"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..546
                     /locus_tag="OO544_004465"
                     /pseudo
     CDS             1..546
                     /locus_tag="OO544_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="deubiquitinase"
BASE COUNT          194 a          120 c           83 g          149 t
ORIGIN      
        1 ccgacttatt aaaaatgcct gtttaaaatt atataattta gcacataccg caaccaactc
       61 ccaccttaaa ccaactggtc cggataattc agatgttctc ctgttcaaaa agctattctc
      121 cccttcgaaa ttaatgacaa ttatcggtga tgaaattcct cttatatctg aaaaacagtc
      181 gctttcaaag gtgcttttaa atgatgagaa taatgaactg agtgatggta caaacttctg
      241 ggataaaaat cgtcaattaa ccacagatga aatagcttgc tatcttcaga agatcgccgc
      301 taatgcaaaa aatactcaag tcaattatcc tactggtctc tacgtcccct actccaccag
      361 aactcacctg gaagacgctc tcaatgaaaa tattaagagc gatccatcat ggccgaatga
      421 agtccagtta ttccccataa atactggcgg acactggata ttagtttcgc tacagaaaat
      481 agtaaataaa aaaataataa actacaaata aaatgcgtca tattcaactc attgcgtgca
      541 ctaggc
//
LOCUS       DAJTZL010000447          520 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.450, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000447 DAJTZL010000000
VERSION     DAJTZL010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..520
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.450"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>520
                     /locus_tag="OO544_004466"
     CDS             <1..>520
                     /locus_tag="OO544_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR6769866.1"
                     /translation="VMRTHFPEYEIISSASAEDLTLLQLRRSGLVIADLAGESEDPRS
                     VCEHYYSLISQYREIHWVFMVSRSWYSQAVELLMCPTATLLSDVEPIENLVKTVRSGN
                     THAERISAMLTSPAMNETHDFSYRSVILTLSERKVLRLLGKGWGINQIASLLKKSNKT
                     ISAQKNSAMRRLA"
BASE COUNT          138 a          121 c          117 g          144 t
ORIGIN      
        1 aggtcatgag gactcacttc cctgaatatg aaataatatc cagcgcctct gcggaggacc
       61 ttacattatt acaattacgt cgttccggat tagtcattgc tgatttagcc ggtgaaagtg
      121 aagatccacg ttctgtttgt gaacattatt attctttaat ctcacaatat cgggaaattc
      181 actgggtttt catggtgtca cgctcctggt attcccaggc agtagaattg ctcatgtgcc
      241 ctacggcgac gttattgtct gatgttgaac ccattgagaa tctggtcaag accgtacgtt
      301 ccggcaatac gcacgcagag cgtatcagcg ccatgctgac ctctccggca atgaatgaaa
      361 ctcatgattt tagctatcgc tccgtcattc tcactctttc agagcgcaag gtactgcggc
      421 tattaggtaa aggatggggc atcaaccaga tagcttcgtt gcttaagaaa agtaataaaa
      481 ctatcagcgc ccaaaaaaac agtgcgatgc gtcgactggc
//
LOCUS       DAJTZL010000448          508 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.451, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000448 DAJTZL010000000
VERSION     DAJTZL010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..508
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.451"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..188)
                     /locus_tag="OO544_004467"
     CDS             complement(<1..188)
                     /locus_tag="OO544_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6769867.1"
                     /translation="MTKNTRFSPEVRQRAIRMVLESQDEYDSQWAAICSIAPKIGCTP
                     ETLRVRVRQHERDTGGGDG"
     gene            242..445
                     /locus_tag="OO544_004468"
                     /pseudo
     CDS             242..445
                     /locus_tag="OO544_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414790.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
BASE COUNT          126 a          135 c          118 g          129 t
ORIGIN      
        1 ccatcaccgc ccccggtatc ccgctcatgc tggcgaaccc ggacacgcag agtctccggc
       61 gtacagccaa tctttggggc aatggaacaa attgccgccc actgtgagtc atattcatcc
      121 tgactttcca gaaccatacg aatcgcccgc tgacggactt cgggggaaaa acgagtattt
      181 ttagtcatcc tgtttacctc tttctcaggg agtttagtct ccaggatttc cggggcggtt
      241 cagacatcta gaatttactg tcagcaccgg cgttaaagtt tacttctgcg atcctcagag
      301 tccttggcag cggggaacaa atgagaatac aaatgggcta attcggcagt actttcctaa
      361 aaagacatgt cttgcccaat atactcaaca tcgagtaggc agcctgacgg ctgcggcttg
      421 tcatggtctg caattaccgt tataaaaaca ggcaatgtca ttgtctttca ggtggttata
      481 tggcccgttc agctaaaccc cgtaaacg
//
LOCUS       DAJTZL010000449          503 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.452, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000449 DAJTZL010000000
VERSION     DAJTZL010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..503
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.452"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            223..>503
                     /locus_tag="OO544_004469"
     CDS             223..>503
                     /locus_tag="OO544_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6769868.1"
                     /translation="MKKVFAKSLLVAAMFSVAGSALAVQKDITVTANVDAALDMTQTD
                     NTALPKAVEMQYLPGQGLQSYQLMTKIWSNDVTKDVKMQLVSPAQLVQSL"
BASE COUNT          172 a           96 c          106 g          129 t
ORIGIN      
        1 cttaaagatt ttaacgatcg cttattgtaa aaataatagc aaagaaaatt gtaagataca
       61 gaccgtaaat aaaaacatct tccttggtca ggaaagaaat cttgaaagca tatctggcta
      121 cgacaaaatc gttgtcaaat ataataactg gatcaccaaa gataatggcg agtttgaact
      181 ggcggtccat taattatcat tgtaataaac gaaggaatag taatgaaaaa ggtatttgca
      241 aaatctcttc tggtcgcagc gatgttttct gttgcaggct ccgcattggc agtacaaaag
      301 gatattactg taacggccaa cgttgatgcc gctctggata tgacacagac cgataacacc
      361 gcgttgccga aagcagtaga aatgcaatat ctgccggggc agggtctgca atcttaccag
      421 ttgatgacca aaatctggtc taacgacgtt accaaagatg taaaaatgca gttagtttcc
      481 ccggcgcaac tggttcaaag cct
//
LOCUS       DAJTZL010000450          500 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.453, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000450 DAJTZL010000000
VERSION     DAJTZL010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..500
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.453"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(1..478)
                     /locus_tag="OO544_004470"
                     /pseudo
     CDS             complement(1..478)
                     /locus_tag="OO544_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
BASE COUNT          168 a          114 c          106 g          112 t
ORIGIN      
        1 tgctagcata aaatggcagc aatggtttga ttaaatgttg aatcttcaca cccgagatag
       61 aacaaccaac aaatagcgca ctacccaccg gcggtgttaa cagcccaata caaaggttgg
      121 ccaccatcat aataccgaaa tgtactgggt ccattcccag ctcttgtgca atcggcaaga
      181 agatcggtgt aaagattagt acagccggtg tcatgtccat aaagatgccg acaattaata
      241 agacgatatt aatcagcagc aatatgatca gtgggttatc agaaatcccc atcaatgcgt
      301 cgctgatcat ataaggaata tcagcattag tcattgccca ggacatgccc acggaaaaac
      361 cgatcaacag taatacaatc gaagtggtaa caaccgactc cagaatcagt ttgggtaaat
      421 ggcgccattt tacttcccgg taaatcagca ccgagagaat aaatgtgtag acaacggcgg
      481 tgatgctgcc aacttactga
//
LOCUS       DAJTZL010000451          489 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.454, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000451 DAJTZL010000000
VERSION     DAJTZL010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..489
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.454"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..>489)
                     /gene="ipaH9.8"
                     /locus_tag="OO544_004471"
     CDS             complement(<1..>489)
                     /gene="ipaH9.8"
                     /locus_tag="OO544_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH9.8"
                     /protein_id="HCR6769869.1"
                     /translation="TGALLSLGREMFRLEILEDIARDKVRTLHFVDEIEVYLAFQTML
                     AEKLQLSTAVKEMRFYGVSGVTANDLRTAEAMVRSREENEFTDWFSLWGPWHAVLKRT
                     EADRWALAEEQKYEMLENEYPQRVADRLKASGLSGDADAEREAGAQVMRETEQQIYRQ
                     LTD"
BASE COUNT           99 a          153 c          117 g          120 t
ORIGIN      
        1 gtcagtcagc tgacggtaaa tctgctgttc agtctcacgc atcacctgtg caccggcttc
       61 cctctccgca tccgcatcac cgctcagacc tgatgctttc agccggtcag ccaccctctg
      121 agggtactca ttctccagca tctcatattt ctgctcttct gccagcgccc agcggtcagc
      181 ttccgtacgc ttcagtacag catgccatgg tccccagagg gagaaccagt ccgtaaattc
      241 attctcttca cggcttctga ccatggcttc ggcagtgcgg aggtcatttg ctgtcactcc
      301 cgacacgcca tagaaacgca tttccttcac ggcagtggag agctgaagtt tctctgcgag
      361 catggtctgg aaggccaggt agacttctat ctcatccaca aaatggagag ttctgacttt
      421 atcccgggca atgtcctcca gaatttcgag gcggaacatt tccctgccca gggagagcag
      481 agcgccggt
//
LOCUS       DAJTZL010000452          486 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.455, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000452 DAJTZL010000000
VERSION     DAJTZL010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..486
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.455"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..486
                     /locus_tag="OO544_004472"
                     /pseudo
     CDS             1..486
                     /locus_tag="OO544_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS1-like element IS1A family transposase"
BASE COUNT          115 a          120 c          145 g          106 t
ORIGIN      
        1 tgcacgcatt atgggcgttg gcctcaacac gattttacgt cacttaaaaa actcaggccg
       61 cagtcggtaa cctcgcgcat acagccgggc agtgacgtca tcgtctgcgc ggaaatggac
      121 gaacagtggg gctatgtcgg ggctaaatcg cgccagcgct ggctgtttta cgcgtatgac
      181 agtctccgga agacggttgt tgcgcacgta ttcggtgaac gcactatggc gacgctgggg
      241 cgtcttatga gcctgctgtc accctttgac gtggtgatat ggatgacgga tggctggccg
      301 ctgtatgaat cccgcctgaa gggaaagctg cacgtaatca gcaagcgata tacgcagcga
      361 attgagcggc ataacctgaa tctgaggcag cacctggcac ggctgggacg gaagtcgctg
      421 tcgttctcaa aatcggtgga gctgcatgac aaagtcatcg ggcattatct gaacataaaa
      481 cactat
//
LOCUS       DAJTZL010000453          476 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.456, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000453 DAJTZL010000000
VERSION     DAJTZL010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.456"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>476
                     /locus_tag="OO544_004473"
     rRNA            <1..>476
                     /locus_tag="OO544_004473"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          119 a          115 c          142 g          100 t
ORIGIN      
        1 tgtcgtcagc tcgtgttgtg aaatgttggg ttaagtcccg caacgagcgc aacccttatc
       61 ctttgttgcc agcggtccgg ccgggaactc aaaggagact gccagtgata aactggagga
      121 aggtggggat gacgtcaagt catcatggcc cttacgacca gggctacaca cgtgctacaa
      181 tggcgcatac aaagagaagc gacctcgcga gagcaagcgg acctcataaa gtgcgtcgta
      241 gtccggattg gagtctgcaa ctcgactcca tgaagtcgga atcgctagta atcgtggatc
      301 agaatgccac ggtgaatacg ttcccgggcc ttgtacacac cgcccgtcac accatgggag
      361 tgggttgcaa aagaagtagg tagcttaacc ttcgggaggg cgcttaccac tttgtgattc
      421 atgactgggg tgaagtcgta acaaggtaac cgtaggggaa cctgcggttg gatcac
//
LOCUS       DAJTZL010000454          467 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.457, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000454 DAJTZL010000000
VERSION     DAJTZL010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..467
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.457"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..225
                     /locus_tag="OO544_004474"
     CDS             <1..225
                     /locus_tag="OO544_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6769870.1"
                     /translation="DSYDNAMAESINGLYKAEVIHRKSWKNRAEVELAILTWVDWYNN
                     RRLLERLGHTPPAEAEKAYYASIGNDDLAA"
     gene            267..467
                     /locus_tag="OO544_004475"
                     /pseudo
     CDS             267..467
                     /locus_tag="OO544_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611683.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
BASE COUNT          150 a          110 c          110 g           97 t
ORIGIN      
        1 gactcgtatg acaacgcgat ggcggagagc atcaatggtc tttacaaagc ggaggtaata
       61 caccgtaaga gctggaaaaa ccgtgcagaa gtggaactgg ccatactcac gtgggtggac
      121 tggtataaca atcgacgatt gctggaaagg ctgggccata ctcctccggc agaagcagaa
      181 aaagcttatt atgcttccat cggaaacgat gatctggcag cctgagttca cagataaaac
      241 actctccagg aaacccgggg cggttcaacc ttcacgataa aaaacttttc attaaaggta
      301 aagcggatca atatccggca cttcattctc tgatatctcc gacccgcatt aatctgaaag
      361 aaatagaaat tcactggcgt gaagtgctac gtctggcgac ctcaataaaa cagggaacag
      421 tgacagcatc cctgatgctg agaaaacttg ccagttaccc caaacaa
//
LOCUS       DAJTZL010000455          465 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.458, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000455 DAJTZL010000000
VERSION     DAJTZL010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..465
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.458"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            43..303
                     /locus_tag="OO544_004476"
     CDS             43..303
                     /locus_tag="OO544_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001523449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6769871.1"
                     /translation="MDQEMTFSLSYEQLTRFAEKRIRECNLDSHGVTYLCESAKAGAV
                     LIFWHELAINGYTSMNAIKRQEIIDADHQRLRKLIWPEDDWK"
BASE COUNT          121 a          124 c          132 g           88 t
ORIGIN      
        1 tgatttttac ctgtcagaac attatcagat ggaacgtgca ggatggatca ggaaatgacg
       61 ttttcactca gctatgaaca actgacccgg tttgcggaaa aacgcatccg ggaatgcaat
      121 ctggacagcc acggcgtaac ttatctctgc gagtcagcca aagccggagc cgtgctgatc
      181 ttctggcatg aactggccat aaatggctat acctcaatga atgccataaa gagacaggag
      241 atcattgacg ccgaccatca gcgcctcaga aagctcatct ggccggagga tgactggaaa
      301 tgactcagag ccgccgaccg tcgccactgc agcggagagt gctgattgtg ctggctgccc
      361 ttgatgaaaa acggccagga cccgtgctga cgcgggacat agaacgggtg ctggagcaaa
      421 gcggggaagc cccggtctac gggccaaacc tgcgcgcctc ctgcc
//
LOCUS       DAJTZL010000456          463 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.459, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000456 DAJTZL010000000
VERSION     DAJTZL010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..463
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.459"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            62..463
                     /locus_tag="OO544_004477"
                     /pseudo
     CDS             62..463
                     /locus_tag="OO544_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
BASE COUNT          119 a          120 c          135 g           89 t
ORIGIN      
        1 cactgcggaa gacgtgaaaa gcacggggaa aaacgccgtc ttatccggtc agcgtgcaca
       61 ggcagccgca ggttatgccc gcgcagcaga acaggccaaa aatgacattg atgctgcgct
      121 gaccggcact ctgaaaacgg ctaaccatct gtctgaaatc gcagcagcag gcgaaaaggc
      181 acaacagaag tcccgggata atctggggct gaaaagtgcg gccacgatgg aagcacagag
      241 cgacatttac gaccggacaa aaggccgtct ggcgataccc ggcgcattcg gctttgggtg
      301 tgcttttctg cctgaagatg ttatccgttt tgacactaag agtgatttcc tggcctgggt
      361 aaggaatgcg ctgccaggtg aatattccgt tgctggcccc tacgacatca tcatacccga
      421 cacacggttt gaaggggtgc tcagcatccg gtggactgat gca
//
LOCUS       DAJTZL010000457          461 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.460, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000457 DAJTZL010000000
VERSION     DAJTZL010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..461
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.460"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..401
                     /locus_tag="OO544_004478"
     CDS             <1..401
                     /locus_tag="OO544_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HCR6769872.1"
                     /translation="YAERDAEIENEKLRREVEELLQASETDLQPGTIEYERHRLTRAQ
                     ADAQELKNARDSAEVVETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFL
                     KRDIIKAMNKAAALDELIPGLLSEYIEQSG"
BASE COUNT          116 a          114 c          139 g           92 t
ORIGIN      
        1 ggtatgccga aagggatgct gaaattgaga acgaaaagct gcgccgggag gttgaagaac
       61 tgctgcaggc cagcgagaca gatctccagc cagggactat tgagtacgaa cgccatcgac
      121 ttacgcgtgc gcaggccgat gcacaggagc tgaaaaatgc cagagactcc gctgaagtgg
      181 tggaaaccgc attctgtact ttcgtgctgt cgcggatcgc aggtgaaatt gccagtattc
      241 tcgacgggat ccccctgtcg gtgcagcggc gttttccgga actggaaaac cgacatgttg
      301 atttcctgaa acgggatatc atcaaagcca tgaacaaagc agccgcgctg gatgaactga
      361 taccggggtt gctgagtgaa tatatcgaac agtcaggtta acaggctgcg gcattttgtc
      421 cgcgccgggc ttcgctcact gttcaggccg gagccacaga c
//
LOCUS       DAJTZL010000458          454 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.461, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000458 DAJTZL010000000
VERSION     DAJTZL010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..454
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.461"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..386
                     /locus_tag="OO544_004479"
     CDS             <1..386
                     /locus_tag="OO544_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HCR6769873.1"
                     /translation="KGQVVELVRLSGRETLRQLEAKTGATRYLMSVLARELVASGDVY
                     NSGYGLFPSEQARKDWQNARKKLSRANLKKPSVVDPDLIWSLPDGEIRRYDRRQNIIC
                     SECRKSEAMQRVLAFYQGNFQKVLL"
BASE COUNT          133 a           95 c          122 g          104 t
ORIGIN      
        1 ttaaagggca ggttgttgaa cttgtacgtc tgagcggtcg cgagacgttg cggcaactgg
       61 aagccaagac aggtgcgaca agatatctga tgagtgttct cgccagagag ctggttgcca
      121 gtggcgatgt atacaactct ggttacgggt tattcccgtc tgaacaggcg cgtaaggact
      181 ggcaaaacgc ccgcaaaaaa ctctcaaggg caaatctgaa gaaaccatct gtggttgatc
      241 ctgaccttat ctggtcatta ccagacggag aaatacgccg ctacgacagg cgtcaaaaca
      301 taatctgtag cgagtgccgg aagagcgaag ctatgcagcg tgtactggct ttctatcagg
      361 gtaattttca gaaggtgctg ttgtgagcca aattaacaat cggaacttcg tgaagagaaa
      421 gcataatcca aaatctgaat aattaagttc agca
//
LOCUS       DAJTZL010000459          442 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.462, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000459 DAJTZL010000000
VERSION     DAJTZL010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..442
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.462"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>442
                     /locus_tag="OO544_004480"
     CDS             <1..>442
                     /locus_tag="OO544_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCR6769874.1"
                     /translation="HRLVHYTRTQYAEPLVESRYLYDPLGRRVAKRVWRRERDLTGWM
                     SLSRKPQVTWYGWDGDRLTTIQNDRTRIQTIYQPGSFTPLIRVETATGELAKTQRRSL
                     ADALQQSGGEDGGSVVFPPVLVQMLDRLESEILADRVSEESRRWL"
BASE COUNT           94 a          127 c          153 g           68 t
ORIGIN      
        1 caccggctgg tgcactacac gcggacacaa tatgcagagc cgctggtcga aagtcgctat
       61 ctttacgacc cgctgggccg cagggtggca aaacgggtat ggcggcgtga acgggacctg
      121 acgggctgga tgtcgctgtc acggaaaccg caagtgacct ggtacggctg ggacggcgac
      181 cggctgacca caatacagaa cgacagaacc cgcatccaga cgatttatca gccggggagc
      241 ttcacgccac tcatcagggt cgaaactgcc accggtgagc tggcgaaaac gcagcgccgc
      301 agcctggcgg atgcccttca gcagtccggc ggcgaagacg gtggcagtgt ggtgttcccg
      361 ccggtgctgg tgcagatgct cgaccggctg gaaagtgaaa tcctggctga ccgggtgagt
      421 gaggaaagcc gccgctggct gg
//
LOCUS       DAJTZL010000460          433 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.463, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000460 DAJTZL010000000
VERSION     DAJTZL010000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..433
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.463"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(211..363)
                     /gene="hokE"
                     /locus_tag="OO544_004481"
     CDS             complement(211..363)
                     /gene="hokE"
                     /locus_tag="OO544_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HCR6769875.1"
                     /translation="MLTKYALAAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
BASE COUNT          112 a          126 c           92 g          103 t
ORIGIN      
        1 ggcacccgcc gttatcttac ccttcctcag caaaatatct gtttatttat acagtaagtc
       61 ctgtttcgga cttctgttgg caacactcgt tattgctcgt aaaacgaaat cgcccgcagc
      121 catcactgac cgcgggcgaa agtgtaaagc aggtgcccta ccatcctgac ctgacaaccg
      181 gatatgcggg gattactccc cgcacaacgg ctacttcttc ggttcgtaag cgagaacagc
      241 cttaaactca atattacgtt ccttcaccgt aaactcacac agcgagtctc cgaccagaag
      301 cgtaaatccc agcaccgtca aacacagcac tatgactgcc gcaagggcat atttcgtcag
      361 catgtttata ttgcctccat gcgaagaagg gactaccatc caaattgttc gggtttggag
      421 tggcagcccc agg
//
LOCUS       DAJTZL010000461          429 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.464, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000461 DAJTZL010000000
VERSION     DAJTZL010000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..429
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.464"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            30..206
                     /locus_tag="OO544_004482"
     CDS             30..206
                     /locus_tag="OO544_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6769876.1"
                     /translation="MSFIFNEETLYFTVFGGDFIMDDLKIVKNTEAWVWYKNCDSKNK
                     KVAVELLQIYELHQ"
BASE COUNT          155 a           48 c          104 g          122 t
ORIGIN      
        1 aaggggtacg aataaagtaa cggggggcag tgagttttat ttttaatgag gaaacattgt
       61 attttactgt ttttggtggg gattttatta tggatgattt aaaaatagta aaaaatacgg
      121 aagcatgggt atggtataaa aattgcgata gtaaaaataa aaaagtggca gttgaacttc
      181 ttcaaatcta cgaattgcat caatgaaaaa tatagttaaa tagaggaggt tattaatagc
      241 aggaaaagca tcacgtgatg ctgatttttt gcactaaata attcgcgcta atgttcaata
      301 gttaatatat ccctcatcag ggaaagatca cggtgtgccg taggcacaaa gatgcaaaac
      361 gggacgtttt gcatgtaaca tgtaataagc ataagcttgt gaagagatgg tggcaggcca
      421 gggaaagat
//
LOCUS       DAJTZL010000462          420 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.465, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000462 DAJTZL010000000
VERSION     DAJTZL010000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.465"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            41..116
                     /locus_tag="OO544_004483"
     tRNA            41..116
                     /locus_tag="OO544_004483"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:74..76,aa:Ile,seq:cat)
BASE COUNT          117 a           79 c           98 g          126 t
ORIGIN      
        1 tttattgcgt ggtttataag tattgttatt gcacactcaa ggccctttag ctcagtggtt
       61 agagcaggcg actcataatc gcttggtcgc tggttcaagt ccagcaaggg ccaccaaatc
      121 attatttacg tatcaggaag agttaagggt acaaggggta ctcgatggcg gtctatattt
      181 catgactaac gccatttcaa ggaagaagtg cggtgggtgg tttcgaatga gggctggtgt
      241 tttggtacgt ggcacgccac tgcaatgtat ttatccatca gtactcattc aaaagtacct
      301 ggaaaaaatg cctgctatgc caagcgggca aattgcttac atgtggaatt aagtaattaa
      361 cgataaagcc agagtattat catttattat accccttcat ttaatcaggt taattactca
//
LOCUS       DAJTZL010000463          398 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.466, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000463 DAJTZL010000000
VERSION     DAJTZL010000463.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..398
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.466"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..363)
                     /locus_tag="OO544_004484"
     CDS             complement(<1..363)
                     /locus_tag="OO544_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105657.1"
                     /note="phosphorylates aminoimidazole ribotide (AIR) which
                     forms 4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate
                     (HMP-P); synthesis of HMP-P from AIR is complex and
                     requires the product of thiC gene at least; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HCR6769877.1"
                     /translation="MPESDGRLLPCPGGAPANVAVGIARLGGISGFIGRVGDDPFGAL
                     MQRTLLTEGVDITYLKQDEWHRTSTVLVDLNDQGERSFTFMVRPSADLFLETTDLPCW
                     RHGEWLHLCSIALSAEPSR"
BASE COUNT          105 a          120 c           89 g           84 t
ORIGIN      
        1 acgcgaaggc tcggcagaca acgcaattga acagagatgt aaccattcgc catgtcgcca
       61 gcagggcaag tctgtcgtct ctaaaaaaag atcggcactg gggcggacca taaacgtaaa
      121 tgaacgttcc ccttgatcgt tcagatcgac aagcaccgtg gatgtccggt gccattcatc
      181 ttgcttcaga tacgtgatat cgactccctc agttagcagc gttctttgca ttaacgcccc
      241 aaaaggatca tcaccgaccc gacctataaa tccacttatt ccgcctaatc tggcgattcc
      301 caccgcaacg ttagctggcg cgccgccagg acaaggcagt aggcgcccgt ctgattctgg
      361 caagagatct acgaccgcat cccctaaaac ccatactt
//
LOCUS       DAJTZL010000464          398 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.467, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000464 DAJTZL010000000
VERSION     DAJTZL010000464.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..398
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.467"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>398
                     /locus_tag="OO544_004485"
     CDS             <1..>398
                     /locus_tag="OO544_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tail tape measure protein"
                     /protein_id="HCR6769878.1"
                     /translation="QSKSLGESLGGLMSDMSHGVDSLTDISYRVRAFFDEVTLAYRSL
                     NDTQKAVADGLANGLLSALKGLAGIVAVRSGIGAVGGIWNLIRAISTLGNVANTAAGR
                     INSRSTGSVEGGKSRLSVADAITRVMIVGM"
BASE COUNT          105 a           64 c          110 g          119 t
ORIGIN      
        1 tcaatctaaa agtcttggtg aatctcttgg tggcttgatg agcgatatgt cgcatggggt
       61 cgattcacta accgatatta gttacagggt acgagcgttc tttgatgagg tgacattagc
      121 ttatcgttca ctaaatgaca ctcaaaaagc tgtagctgat ggtcttgcaa atggcctgtt
      181 aagtgcatta aaggggcttg ctggtatagt cgctgtacgg tctgggattg gtgctgtagg
      241 tggtatctgg aacttaatta gggccatttc cacgcttgga aatgtggcaa atacagcagc
      301 aggaagaatc aattcacgtt ctactggtag tgttgaaggt ggaaaaagta ggctatctgt
      361 agctgatgca attaccagag taatgattgt cggtatga
//
LOCUS       DAJTZL010000465          396 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.468, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000465 DAJTZL010000000
VERSION     DAJTZL010000465.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..396
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.468"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(101..>396)
                     /locus_tag="OO544_004486"
     CDS             complement(101..>396)
                     /locus_tag="OO544_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HCR6769879.1"
                     /translation="LVVELVLSAGFLLVIHGATDKFAPAGFAPIAIGLALTLIHLISI
                     PVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGIIGGLIYRTLLEKRD"
BASE COUNT          120 a          106 c           93 g           77 t
ORIGIN      
        1 atgtggctta gctcatgaaa ggagcgcaac aagatgatga atcaacatat aacaacatct
       61 taaaaaaagg cctgacatta cgccaggcct tctgcgttaa ttaatcacgc ttttccagca
      121 gggtccggta aatcagacca ccgataatgc cgccgacaat tggcaccacc cagaagaacc
      181 acagttgttc taatgcccag ccgccctgga agatagcaac cgcggtgctg cgcgccgggt
      241 taacagaagt gttagtcacc ggaatactaa ttaagtgaat cagggttaag gccagaccaa
      301 tagcgatcgg cgcaaaacct gccggcgcga atttgtcggt tgcgccgtgg atcaccaaca
      361 ggaaacctgc actcaatacc agttcaacta ccagcg
//
LOCUS       DAJTZL010000466          395 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.469, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000466 DAJTZL010000000
VERSION     DAJTZL010000466.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..395
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.469"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            229..336
                     /locus_tag="OO544_004487"
     CDS             229..336
                     /locus_tag="OO544_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HCR6769880.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
BASE COUNT           98 a           98 c          119 g           80 t
ORIGIN      
        1 gagacggatt ccggaacaga aaaacagtcg tagaatcagc gtcaagccgg aaggattccg
       61 gtgaggcgca atgttgcggg ggcttaatcc ctggtggcat tggttgctgg agagagaaac
      121 cccccgcacg ttgcaggtat gcacctgaca acaccacggg ggccattctt gcatccaaac
      181 aatacaagaa tagccgcgaa acgttgtcat tacaatacag gcggctatat gacgctcgca
      241 gaactgggca tggccttctg gcatgattta gcggctccgg tcattgctgg tattcttgcc
      301 agtatgatcg tgaactggct gaacaagcgg aagtaacgtg ccatgcgggc gtcaggctgc
      361 cgtaatggca atttgcgccc ggatcaggcc gcagg
//
LOCUS       DAJTZL010000467          390 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.470, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000467 DAJTZL010000000
VERSION     DAJTZL010000467.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..390
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.470"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..>390)
                     /locus_tag="OO544_004488"
     CDS             complement(<1..>390)
                     /locus_tag="OO544_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR6769881.1"
                     /translation="IYTPLTLKLYDWWVLGVSNRLAWGCPTKEHLLPHFLEHLGNNHL
                     DIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHKISHDVFEPYPAALH
                     GQFDSISMFYLLHCLPGNISTKSCVIR"
BASE COUNT          120 a           78 c           82 g          110 t
ORIGIN      
        1 ttgcgtatta cacagctttt tgtagatata tttccaggca ggcagtgaag aaggtaaaac
       61 atggaaatgg aatcaaattg accatgtaac gccgcgggat aaggttcaaa aacatcatgg
      121 ctaattttat gtttaatttt tgattcccca gcccttgtag atgccgcgtt caggctagct
      181 tcgttcaaat ccattaaaga tatcagacta ctctcaggta cgtgagtaag gtaaaaccca
      241 gttccaacac caatatccag atggttgtta cctaaatgtt ccagaaagtg tggaagaagg
      301 tgttcctttg taggacatcc ccatgcaagc cgatttgata ctcccaaaac ccaccagtca
      361 taaagcttta gggtaagtgg tgtgtaaatt
//
LOCUS       DAJTZL010000468          389 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.471, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000468 DAJTZL010000000
VERSION     DAJTZL010000468.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.471"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            251..>389
                     /locus_tag="OO544_004489"
     CDS             251..>389
                     /locus_tag="OO544_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="activates the csgBA and csgDEFG operons involved in
                     biofilm formation; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HCR6769882.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RS"
BASE COUNT          121 a           69 c           65 g          134 t
ORIGIN      
        1 ctaaacttaa taaaacctta agattaacat tttaatataa cgagttgcat ttagttacat
       61 gtttaacact tgatttaaga tttgtaatgg ctagattgaa atcagatgta atccattagt
      121 tttatatttt acccatttag ggctgattta ttactacaca cagcagtgca acatctgtca
      181 gtacttctgg tgcttctatt ttagaggcag ctgtcaggtg tgcgatcaat aaaaaaagcg
      241 gggtttcatc atgtttaatg aagtccatag tattcatggt catacattat tgttgatcac
      301 taaaccttct ttgcaggcga cggctctctt gcagcacctt aaacaatcgc tggcaattac
      361 aggaaaatta cataatattc aacgttctc
//
LOCUS       DAJTZL010000469          386 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.472, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000469 DAJTZL010000000
VERSION     DAJTZL010000469.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.472"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>386
                     /locus_tag="OO544_004490"
     CDS             <1..>386
                     /locus_tag="OO544_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6769883.1"
                     /translation="LYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAYFGLPEALMNR
                     FVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPARAAWIQFLIV
                     IPLMIIPMLGSNTVQDLMNTIINMTA"
BASE COUNT           81 a           98 c          102 g          105 t
ORIGIN      
        1 actgtattcc gtctcctcgg tgctgattaa cgtcttcgtc agcagcaaag agttgaaatt
       61 taccggcgga tcggtgcagg tattccacgg catggcggcg tattttggtc taccggaagc
      121 gttgatgaat cgctttgtcg gtctggtgtc ctttaccgcg atgttcggtt ccctgctgat
      181 gtggaccgca acgccggtga aaattttctt ctccgaaatc ccggaaggca tctttggtaa
      241 gaaaaccgtc gaactgaacg aaaacggcgt tccggcgcgc gcagcgtgga tccagttcct
      301 gatcgtcatc ccgctgatga ttatcccgat gctcggttcc aataccgtgc aggatctgat
      361 gaatactatt attaatatga ccgccg
//
LOCUS       DAJTZL010000470          360 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.473, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000470 DAJTZL010000000
VERSION     DAJTZL010000470.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..360
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.473"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>360
                     /locus_tag="OO544_004491"
     CDS             <1..>360
                     /locus_tag="OO544_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6769884.1"
                     /translation="IGKDVKERATKSYDEAEYVVDKKTGKGIWKGGDSGINTLMNEGF
                     LTKKDLKDFANNSAGLISKAVNGMVKKGYTDAQIGSRLEDLADDLLLTSKYWQRSARE
                     VQASITQQKAAGQWLTET"
BASE COUNT          127 a           65 c           89 g           79 t
ORIGIN      
        1 catcggtaaa gatgtaaagg aacgcgctac caaaagctat gacgaggctg aatatgtagt
       61 tgataagaaa acagggaaag gcatatggaa gggcggtgat tctggtatta acactttaat
      121 gaatgaagga ttcttaacta aaaaggattt gaaagacttt gccaataact ctgctggttt
      181 aatttcaaaa gctgtcaacg gaatggtgaa aaagggctat accgacgcac agatcggtag
      241 tcgtctggaa gatttagcgg acgacctcct cttaacttct aaatattggc aaagatcagc
      301 cagagaggtg caggcaagca ttacccagca aaaagccgcc ggacaatggc taacagaaac
//
LOCUS       DAJTZL010000471          353 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.474, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000471 DAJTZL010000000
VERSION     DAJTZL010000471.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..353
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.474"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..>353)
                     /locus_tag="OO544_004492"
     CDS             complement(<1..>353)
                     /locus_tag="OO544_004492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001351448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCR6769885.1"
                     /translation="QYSVILLVEGFPPSHAGTITVYEDSRPGTLNDFLGAMTEDDVRP
                     EALRRFELMVEEVARNASAVAQNTAAAKKSASDAGTSAREAATHATDAAGSARAASTS
                     AGQAASSAQSASSSAG"
BASE COUNT           63 a          115 c          102 g           73 t
ORIGIN      
        1 cctgcgctgg aagacgctga ctgagccgac gacgcggcct gtccggctga cgtgctggct
       61 gcgcgtgctg agcctgctgc atcagtcgca tgggttgccg cctcacgggc tgatgtgccg
      121 gcatcgctgg ctgacttctt cgcggctgcc gtgttctgtg ccactgcgga cgcgttacgc
      181 gccacctctt ccaccatcag ttcaaaacgt cgcaatgcct ccggacggac atcatcctcc
      241 gtcatggcac caagaaaatc attcagcgta ccgggtcggg aatcttcata cacggtgatg
      301 gtcccggcat gtgacggcgg gaatccctcc accaacagaa taacgctgta ctg
//
LOCUS       DAJTZL010000472          352 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.475, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000472 DAJTZL010000000
VERSION     DAJTZL010000472.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..352
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.475"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            63..>352
                     /gene="yhaV"
                     /locus_tag="OO544_004493"
     CDS             63..>352
                     /gene="yhaV"
                     /locus_tag="OO544_004493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ribonuclease
                     toxin YhaV"
                     /protein_id="HCR6769886.1"
                     /translation="MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKA
                     ATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKNKDWSRVKFGAGRYRLF"
BASE COUNT          105 a           68 c           89 g           90 t
ORIGIN      
        1 ggaaagaaac ttgtcactgg catggacgtc aacattgatg atgagattgg cgacgacgaa
       61 taatggattt tccacaaagg gttaatggtt gggcgctata tgctcatccc tgttttcagg
      121 aaacctacga cgctttagtt gccgaagtcg agacattaaa gggaaaagat cctgaaaatt
      181 atcagagaaa agccgccaca aagttattgg cggtagtcca taaagtgatt gaggagcata
      241 tcacggtcaa tccatcatca ccggcattcc gtcatggcaa gtcgttaggc tctgggaaaa
      301 ataaagactg gtcacgggta aaatttggtg caggtcgtta tcgtctcttc tt
//
LOCUS       DAJTZL010000473          344 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.476, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000473 DAJTZL010000000
VERSION     DAJTZL010000473.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.476"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>344
                     /locus_tag="OO544_004494"
     CDS             <1..>344
                     /locus_tag="OO544_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCR6769887.1"
                     /translation="LQSQHLNSLLSDRDYTWNDNGELIRISSPRQTRSYSYSTTGRLT
                     GVHTTAANLDIRIPYATDPAGNRLPDPELHPDSTLSMWPDNRIARDAHYLYRYDHHGR
                     LTEKTDLIPEGV"
BASE COUNT           83 a          124 c           89 g           48 t
ORIGIN      
        1 gttacagagc cagcacctga acagcctgct gtctgaccgc gattacacct ggaacgacaa
       61 cggcgaactc atccgcatca gcagcccgcg ccagacccgg agttacagct acagcaccac
      121 cggcaggctg accggcgttc acaccaccgc agcgaatctg gatatccgca tcccgtatgc
      181 cacagacccg gcggggaacc gcctgcccga cccggagctg cacccggaca gcaccctcag
      241 catgtggccg gataaccgta tcgcccgtga cgcgcactat ctttaccggt atgaccatca
      301 cggcagactg acagagaaaa ccgacctcat cccggaaggg gtta
//
LOCUS       DAJTZL010000474          339 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.477, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000474 DAJTZL010000000
VERSION     DAJTZL010000474.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..339
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.477"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..261
                     /gene="istB"
                     /locus_tag="OO544_004495"
     CDS             <1..261
                     /gene="istB"
                     /locus_tag="OO544_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISSso4 family helper ATPase
                     IstB"
                     /protein_id="HCR6769888.1"
                     /translation="LDDLGLEQLSNAQCNDLLEITEDRYGQSSTIVVSQFPVDKWHGL
                     MENPTTADAILDRLVHNSHRVVLQGESLRKNPPTVESSEKTS"
BASE COUNT          108 a           74 c           93 g           64 t
ORIGIN      
        1 ctggacgatc tggggctgga acagttaagt aacgcccagt gtaatgacct tctggagatc
       61 acagaggatc gttacggaca aagcagcacg atagtggtaa gccagttccc ggtagataaa
      121 tggcatggtc tgatggagaa cccaacaaca gcagatgcca tcctggacag actggtacat
      181 aactcacaca gagtggtcct tcagggagag tccctgagga aaaatccccc gacagtggaa
      241 agcagcgaaa aaacgagtta aaaagcggta gaaaaagtta agttcccggt tgatcgaatg
      301 gatgcgaatc gctgatcgaa cttcgatgcc aatccgtga
//
LOCUS       DAJTZL010000475          337 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.478, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000475 DAJTZL010000000
VERSION     DAJTZL010000475.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..337
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.478"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>337
                     /locus_tag="OO544_004496"
     CDS             <1..>337
                     /locus_tag="OO544_004496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6769889.1"
                     /translation="NDLPSSPVEWLTDNGSCYRANETRQFARMLGLEPKNTAVRSPES
                     NGIAESFVKTIKRDYISIMPKPDGLTAAKNLAEAFEHYNEWHPHSALGYRSPREYLRQ
                     RACNGLSDN"
BASE COUNT           91 a           77 c          102 g           67 t
ORIGIN      
        1 gcaacgatct tccgtcgtct ccagtggagt ggctgacgga taatggttca tgctaccggg
       61 ctaatgaaac acgccagttc gcccggatgt tgggacttga accgaagaac acggcggtgc
      121 ggagtccgga gagtaacgga atagcagaga gcttcgtgaa aacgataaag cgtgactaca
      181 tcagtatcat gcccaaacca gacgggttaa cggcagcaaa gaaccttgca gaggcgttcg
      241 agcattataa cgaatggcat ccgcatagtg cgctgggtta tcgctcgcca cgggaatatc
      301 tgcggcagcg ggcttgtaat gggttaagtg ataacag
//
LOCUS       DAJTZL010000476          331 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.479, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000476 DAJTZL010000000
VERSION     DAJTZL010000476.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..331
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.479"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(30..279)
                     /locus_tag="OO544_004497"
                     /pseudo
     CDS             complement(30..279)
                     /locus_tag="OO544_004497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415685.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          111 a           63 c           57 g          100 t
ORIGIN      
        1 tctggagatt cagggggcca gtctaagttc taagagagca cggattccct gtcattaata
       61 atgatatccg ttagtgtagt cttatgcatt tcaatgtact gcaatatttg cagatagaga
      121 tcagaaagaa tcacactggt ttcatctttt aaacgcaagc gtaaagcctg atcaataata
      181 tgactatcaa catacccatg cgtcatgaac acctccctct cgagattttt aaacaaagca
      241 cattgaaaaa ataaatctct ctgggattga attattcatg tcagcctcgt agaaatatta
      301 acctaataaa attatgctgt gtatagtcta a
//
LOCUS       DAJTZL010000477          321 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.480, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000477 DAJTZL010000000
VERSION     DAJTZL010000477.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.480"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>321
                     /locus_tag="OO544_004498"
     CDS             <1..>321
                     /locus_tag="OO544_004498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HCR6769890.1"
                     /translation="VQIRQNQPHAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVN
                     GHAHLLYALNIAVRTAPDSSVKALKYAAAIERSLCEKLCADVNYSGLICKNPFHLEWQ
                     VMEWR"
BASE COUNT           72 a           84 c           95 g           70 t
ORIGIN      
        1 tgtccagatc cggcagaacc agcctcatgc gcagttctgg ctggtttttg acgttgatcg
       61 cgagggggca gcgattgact ggagcgaccg gaacgctccc gcgccgaaca tcaccgtaaa
      121 aaatcctgtg aacggtcatg ctcacctgct ctacgcgctc aatatcgccg tgagaaccgc
      181 gccggacagc tcggttaagg cgctgaaata cgccgctgcg attgaacgtt cgctgtgtga
      241 gaaactttgt gctgatgtga actacagcgg actgatttgt aaaaatccgt tccacctgga
      301 atggcaggtg atggagtggc g
//
LOCUS       DAJTZL010000478          317 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.481, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000478 DAJTZL010000000
VERSION     DAJTZL010000478.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..317
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.481"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..217)
                     /locus_tag="OO544_004499"
     CDS             complement(<1..217)
                     /locus_tag="OO544_004499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HCR6769891.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQPYPIQGSGFELNGKG"
     gene            complement(217..>317)
                     /locus_tag="OO544_004500"
     CDS             complement(217..>317)
                     /locus_tag="OO544_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6769892.1"
                     /translation="EWRQIKVTCAKWSSRVSMLRVEFSAEFEQVVN"
BASE COUNT           67 a          108 c           61 g           81 t
ORIGIN      
        1 tgcctttgcc attcagttca aaaccactcc cctgaatggg atacggctga tactgtcgcc
       61 cctgccaggt gaccggctca cctttttcgt tctgctcatt acagaaaaaa taacgttctc
      121 caccgacctc tgtcagatcg atttcccaga gcaccacgct ggccgactgc tccgcacggg
      181 tgcattcatt cagtgtttcc tgccggatat cctgcatcag ttcaccacct gttcaaactc
      241 tgcgctgaac tcaacacgca gcatactgac ccgcgacgac cattttgcgc aggtcacctt
      301 tatctgccgc cactcat
//
LOCUS       DAJTZL010000479          316 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.482, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000479 DAJTZL010000000
VERSION     DAJTZL010000479.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..316
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.482"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            71..146
                     /locus_tag="OO544_004501"
     tRNA            71..146
                     /locus_tag="OO544_004501"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:105..107,aa:Glu,seq:ttc)
BASE COUNT           86 a           72 c           75 g           83 t
ORIGIN      
        1 ctttgcagtg ctcacacaga ttgtctgata gaaagtgaaa agcaaggcgt cttgcgaagc
       61 agactgatac gtccccttcg tctagaggcc caggacaccg ccctttcacg gcggtaacag
      121 gggttcgaat cccctagggg acgccacttg ctggtttgtg agtgaaagtc acctgcctta
      181 atatctcaaa actcatcttc gggtgatgtt tgagatattt gctctttaaa aatctggatc
      241 aagctgaaaa ttgaaacact gaacaacgaa agttgttcgt gagtctctcg aattttcgca
      301 acacgatgat gaatcg
//
LOCUS       DAJTZL010000480          312 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.denovo.483, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000480 DAJTZL010000000
VERSION     DAJTZL010000480.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..312
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.denovo.483"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(1..76)
                     /locus_tag="OO544_004502"
     tRNA            complement(1..76)
                     /locus_tag="OO544_004502"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(41..43),aa:Thr,seq:tgt)
     gene            complement(79..155)
                     /locus_tag="OO544_004503"
     tRNA            complement(79..155)
                     /locus_tag="OO544_004503"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(119..121),aa:Arg,seq:tct)
     gene            complement(157..233)
                     /locus_tag="OO544_004504"
     tRNA            complement(157..233)
                     /locus_tag="OO544_004504"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(197..199),aa:Ile,seq:cat)
BASE COUNT           71 a           87 c           81 g           73 t
ORIGIN      
        1 tggtgccgac taccggaatc gaactggtga cctactgatt acaagtcagt tgctctgcct
       61 gctgagctaa gtcggcgctg gcccaccacc gaggactcga acctcgcacc gtcaacttag
      121 aaggttgatg ctctatccgg atgagctagt ggtggttggt ggcccttgct ggacttgaac
      181 cagcgacctg gcgattatga gtcgctcgct ctgaccaact gagctaaagg gccggaggca
      241 gaataataac catatgtcat cacatctgca aactcatctg accaccagca cgtttaacgt
      301 cctgtgccgt tt
//
LOCUS       DAJTZL010000481         7022 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.guided.188, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000481 DAJTZL010000000
VERSION     DAJTZL010000481.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7022
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.guided.188"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..623
                     /locus_tag="OO544_004505"
     CDS             <1..623
                     /locus_tag="OO544_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="part of four member fumarate reductase enzyme
                     complex FrdABCD which catalyzes the reduction of fumarate
                     to succinate during anaerobic respiration; FrdAB are the
                     catalytic subcomplex consisting of a flavoprotein subunit
                     and an iron-sulfur subunit, respectively; FrdCD are the
                     membrane components which interact with quinone and are
                     involved in electron transfer; the catalytic subunits are
                     similar to succinate dehydrogenase SdhAB; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     flavoprotein subunit"
                     /protein_id="HCR6769893.1"
                     /translation="LAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDL
                     VNQDGGENWAKIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQECFKRVRITDTSSV
                     FNTDLLYTIELGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFR
                     DADGTTRLEYSDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            616..1350
                     /gene="frdB"
                     /locus_tag="OO544_004506"
     CDS             616..1350
                     /gene="frdB"
                     /locus_tag="OO544_004506"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HCR6769894.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALVYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            1361..1756
                     /gene="frdC"
                     /locus_tag="OO544_004507"
     CDS             1361..1756
                     /gene="frdC"
                     /locus_tag="OO544_004507"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HCR6769895.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            1767..2126
                     /gene="frdD"
                     /locus_tag="OO544_004508"
     CDS             1767..2126
                     /gene="frdD"
                     /locus_tag="OO544_004508"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HCR6769896.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            2189..3322
                     /locus_tag="OO544_004509"
     CDS             2189..3322
                     /locus_tag="OO544_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HCR6769897.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLTPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            3411..3944
                     /gene="blc"
                     /locus_tag="OO544_004510"
     CDS             3411..3944
                     /gene="blc"
                     /locus_tag="OO544_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HCR6769898.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(3941..4258)
                     /gene="sugE"
                     /locus_tag="OO544_004511"
     CDS             complement(3941..4258)
                     /gene="sugE"
                     /locus_tag="OO544_004511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HCR6769899.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(4433..4579)
                     /gene="ecnB"
                     /locus_tag="OO544_004512"
     CDS             complement(4433..4579)
                     /gene="ecnB"
                     /locus_tag="OO544_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HCR6769900.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(4690..4815)
                     /gene="ecnA"
                     /locus_tag="OO544_004513"
     CDS             complement(4690..4815)
                     /gene="ecnA"
                     /locus_tag="OO544_004513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HCR6769901.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(4867..5433)
                     /gene="efp"
                     /locus_tag="OO544_004514"
     CDS             complement(4867..5433)
                     /gene="efp"
                     /locus_tag="OO544_004514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HCR6769902.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            5475..6503
                     /gene="epmB"
                     /locus_tag="OO544_004515"
     CDS             5475..6503
                     /gene="epmB"
                     /locus_tag="OO544_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HCR6769903.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPGELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPILRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            6893..6988
                     /locus_tag="OO544_004516"
                     /pseudo
     CDS             6893..6988
                     /locus_tag="OO544_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313153.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1785 a         1771 c         1794 g         1672 t
ORIGIN      
        1 ccctggcgga actggtggtc ttcggccgtc tggccggtga acaagcgaca gagcgtgcag
       61 caactgccgg taatggcaac gaagcggcaa ttgaagcgca ggcagctggc gttgaacaac
      121 gtctgaaaga tctggttaac caggatggcg gcgaaaactg ggcgaagatc cgcgacgaaa
      181 tgggcctggc aatggaagaa ggttgcggta tctaccgtac gccggaactg atgcagaaaa
      241 ccatcgacaa gctggcagag ctgcaggaat gcttcaagcg cgtgcgcatc accgacacct
      301 ccagcgtgtt caacaccgac ctgctctaca ccattgaact gggccacggt ctgaacgttg
      361 ctgaatgtat ggcgcactcc gcaatggcac gtaaagagtc ccgcggcgca caccagcgtc
      421 tggacgaagg ttgcaccgag cgtgacgacg tcaacttcct caaacacacc ctcgccttcc
      481 gcgatgctga tggcacgact cgcctagagt acagcgacgt gaagattact acgctgccgc
      541 cagctaaacg cgtttacggt ggcgaagcgg atgcagccga taaggcggaa gcagccaata
      601 agaaggagaa ggcgaatggc tgagatgaaa aacctgaaaa ttgaggtggt gcgctataac
      661 ccggaagtcg ataccgcacc gcatagcgca ttctatgaag tgccttatga cgcaactacc
      721 tcattactgg atgcgctggt ctacatcaaa gacaacctgg caccggacct gagttaccgc
      781 tggtcctgcc gtatggcgat ttgtggttcc tgcggcatga tggttaacaa cgtgccaaaa
      841 ctggcatgta aaaccttcct gcgtgattac accgacggta tgaaggttga agcgttagct
      901 aacttcccga ttgaacgcga tctggtggtc gatatgaccc acttcatcga aagtctggaa
      961 gcgatcaaac cgtacatcat cggcaactcc cgcaccgcgg atcagggtac taacatccag
     1021 accccggcgc agatggcaaa gtatcaccag ttctccggtt gcatcaactg tggtctgtgc
     1081 tacgccgcgt gcccgcagtt tggcctgaac ccagagttca tcggtccggc tgccattacg
     1141 ctggcgcatc gttataacga agatagccgc gaccacggta agaaggagcg tatggcgcag
     1201 ttgaacagcc agaacggcgt atggagctgt actttcgtgg gctattgctc cgaagtctgc
     1261 ccgaaacacg tcgatccggc tgcggccatt cagcagggca aagtagaaag ttcgaaagac
     1321 tttcttatcg cgaccctgaa accacgctaa ggagtgcaac atgacgacta aacgtaaacc
     1381 gtatgtacgg ccaatgacgt ccacctggtg gaaaaaattg ccgttttatc gcttttacat
     1441 gctgcgcgaa ggcacggcgg ttccggctgt gtggttcagc attgaactga ttttcgggct
     1501 gtttgccctg aaaaatggcc cggaagcctg ggcgggattc gtcgactttt tacaaaaccc
     1561 ggttatcgtg atcattaacc tgatcactct ggcggcagct ctgctgcaca ccaaaacctg
     1621 gtttgaactg gcaccgaaag cggccaatat cattgtaaaa gacgaaaaaa tgggaccaga
     1681 gccaattatc aaaagtctct gggcggtaac tgtggttgcc accatcgtaa tcctgtttgt
     1741 tgccctgtac tggtaaggag cctgagatga ttaatccaaa tccaaagcgt tctgacgaac
     1801 cggtattctg gggcctcttc ggggccggtg gtatgtggag cgcaattatt gcgccggtga
     1861 tgatcctgct ggtgggtatt ctgctgccac tggggctgtt tccgggtgat gcgctgagct
     1921 acgagcgcgt tctggcgttc gcgcagagct tcattggtcg cgtattcctg ttcctgatga
     1981 tcgttctgcc gctgtggtgt ggtttacacc gtatgcacca cgcgatgcac gatctgaaaa
     2041 ttcacgtgcc tgcgggcaaa tgggttttct acggtctggc tgctatcctg acagttgtca
     2101 cgctgattgg tatcgttaca atctaacgta tcgccaatgt aaatccggcc cgcctatggc
     2161 gggccgtttt gtatggaaac cagaccttat gttcaaaacg acgctctgcg ccttattaat
     2221 taccgcctct tgctccacat ttgctgcccc tcaacaaatc aacgatattg tgcatcgcac
     2281 aattaccccg cttatagagc aacaaaagat ccccggtatg gcggtggcgg taatttatca
     2341 gggtaaacct tattacttta cctggggcta tgcggacatc gccaaaaagc agcccgtcac
     2401 acagcaaacg ttgtttgagt taggttcggt cagcaaaaca tttacgggcg tgcttggtgg
     2461 cgacgctatt gctcgagggg aaatcaagtt aagcgatccc gcaacaaaat actggcctga
     2521 acttactgct aaacagtgga atgggatcac actattacat cttgcgacct acaccgctgg
     2581 cggcctgcca ttgcaggtgc cggatgaagt gaaatcctca agcgacttgc tgcgcttcta
     2641 tcaaaactgg cagcctgcat gggctccagg aacacaacgt ctgtatgcca actccagtat
     2701 cggtttgttc ggcgcactgg ctgtgaagcc gtctggtttg agttttgagc aggcgatgca
     2761 aactcgtgtc ttccagccac tcaaactcaa ccatacgtgg attaatgtac ctcccccaga
     2821 agaaaagaat tacgcctggg gatatcgcga aggtaaggca gtgcatgttt cgccaggggc
     2881 gttagatgct gaagcttatg gtgtgaagtc gaccattgaa gatatggccc gctgggtgca
     2941 aagcaattta accccccttg atatcaatga gaaaacgctt caacaaggga tacaactggc
     3001 acaatctcgc tactggcaaa ccggcgatat gtatcagggc ctgggctggg aaatgctgga
     3061 ctggccggtc aatcctgaca gcatcattaa cggcagtgac aataaaattg cactggcagc
     3121 acgcccggta aaagcgatta agcccccaac tcctgcagta cgcgcatcat gggtacataa
     3181 aacgggggcg accggcggat ttggtagcta tgtcgcgttt attccagaaa aagagctggg
     3241 tatcgtgatg ctggctaaca aaaactatcc caatccagcg agagtcgccg ccgcctgtca
     3301 gattctcaac gctctacagt aaaatttcat cgggtccgaa ttttcggacc ttttctgcgc
     3361 ttttccttgc tgtcatctac acttagaaaa aaaccagtaa ggaaacattt atgcgcctgc
     3421 tccctctcgt tgccgcagcg acagctgcat ttctggtcgt tgcctgcagt tctcctacgc
     3481 cgccgcgtgg cgtgacagta gtgaataatt tcgacgctaa acgctatctc ggtacctggt
     3541 atgagattgc ccgttttgat caccgttttg aacgtggact ggaaaaagtc accgcaacat
     3601 acagtctgcg tgatgacggc ggcctgaatg tcatcaataa aggctataac cctgacagag
     3661 aaatgtggca gcagagtgaa gggaaagcgt actttaccgg cgcaccaaca cgcgctgcgc
     3721 tgaaagtgtc attctttggt cctttctatg gcggttataa cgtcattgca cttgatcggg
     3781 aataccgcca tgcgctggtt tgcggcccgg accgcgacta cctgtggata ctctcccgca
     3841 cgccaaccat ttctgacgaa gtgaaacagg agatgctggc agtcgcgacc cgggaagggt
     3901 ttgatgtcag taagtttatt tgggtacagc agcccggtag ttagtgagtg ctgagtttca
     3961 gaccaataat ccccaatacg attaacgcca gactcgccag gcgcatcggg ttagcggact
     4021 caccgagcag cacaatgccg gtgatggccg cgccgactgc gccaataccc gtccacacgg
     4081 cataagccgt ccctaccggt aacgatttca tcgcccaggc aagtagcgcc atactgacta
     4141 tcatcgccgt cacagtaata acacttggcg tcaaacgact aaagccgtgg gtatatttca
     4201 ggccaacggc ccataccact tccagcagac cagcaataac taagataatc caggacatat
     4261 caggctccgg agaattgggg ccatccccgg tgaaaagagg cgtttgcagg tcgtcctgca
     4321 aagctgatgg ttgaacggat attttgccag gtggcagggg aatttcaact attacctttg
     4381 tgactatcgc aaaaaaacaa acggcacgac ataactgtcg taccgttatt gcttattgct
     4441 gcgctttcgt tgctgcacca gaaatcgcgt taccgccatc agaaatgtct tcaccaacgc
     4501 cacgcgtggt gttgcaggca gttaatactg ttgaaagcac cagaacagaa aagatcgctg
     4561 caattgtctt cttcaccata acgtcttcct tttaggtaat gttatttatg tttgcctata
     4621 gcaaattaat aatagacaac atcgccaaaa atgatggaaa tctgatgatt tttcggaaga
     4681 gaagaaaaat tagctggcag cgcgggagat ggagttgccg agatgtttga tgtcttcgcc
     4741 gaaaccgcga gcggtgttac agcccgtgag cagcgtgctg gcaagcaaaa caagaacgat
     4801 aaggcgtttc atcatctctg acctgaataa gtgatggtgc agcctgcagg ccgcaccaca
     4861 accgcattac ttcacgcgag agacgtattc accagagcgg gtatccactt tgatgacttc
     4921 gccgatttgt acgaacagcg gaactttaac cacagcgcca gtagacaggg tagccggttt
     4981 gccgccagta cctgcggtat cacctttcag gcccggatcg gtatcaacga tttccagttc
     5041 aacgaagttc ggcggagtaa cggagatcgg ctgaccattc cacagagtta cgatacactc
     5101 tgcctgatcc agcagccatt tagcgttgtc gccaattgct tttgcatcag cagacagctg
     5161 ctcgaaagtt tcgttgttca tgaagtgcca gaactcaccg tcgttgtaca ggtaagtcag
     5221 gttcatatcg acaacatcag cgccttcagc ggaatcagta gatttgaagg ttttttctac
     5281 gcgagtaccg gtcagcagac gacgcagttt aacgcgagca aatgcctggc ctttacccgg
     5341 ttttacgaat tcactcgctt caaccgcgta aggttcgccg tctaacatga ttttaagacc
     5401 agcacgaaaa tcgttgctat agtacgttgc cataaggccc tctgaaattt gttaattggt
     5461 agctaagcca caaaatggcg catattgtaa ccctaaatac cccatccaga gaagattggt
     5521 taacgcaact tgccgatgtt gtgaccgatc ctggtgaact tctgcgtctt ttgaatatag
     5581 acgcggacga aaaactgttg gccggacgca gcgccaaaaa gctgtttgcc ctgcgtgtgc
     5641 cccgctcatt tatcgatcgc atggagaaag gcaatccgga cgatcctatt ttgcgtcagg
     5701 tacttacctc gcaagatgag tttgtcgtcg cgtccggatt ctccaccgac ccactggaag
     5761 aacagcacag cgtagtgcct ggtttgttgc ataaatacca caaccgggcg cttttgctgg
     5821 tcaaaggcgg ctgcgcggta aattgccgct attgcttccg tcgccacttc ccctatgccg
     5881 aaaatcaggg caacaagcgt aactggcaga ctgcacttga gtatgttgct gcgcatccgg
     5941 aactggacga gatgattttc tccggcggcg atccgctgat ggcgaaagat cacgagctgg
     6001 actggttgct cacacaactg gaagccatcc cgcatatcaa acgtctgcgg attcacagcc
     6061 gtctgccgat tgtgatcccg gcacgtatca ccgaggcgct ggttgaacgc tttgcccgtt
     6121 ctacgctgca aatcttgctg gtgaatcaca tcaatcatgc caatgaggta gatgaaacgt
     6181 tccgtcaggc gatggctaaa ttgcgccgtg tcggtgtcac cctgcttaac cagagcgttc
     6241 tgttacgtgg tgtgaacgat aacgcacaaa cgttggcaaa cctgagtaat gcgttgttcg
     6301 atgccggcgt gatgccgtat tacctgcatg tgctagataa agtacagggc gcggcgcatt
     6361 ttatggtgag tgatgacgaa gcacggcaga ttatgcgtga gttgctgaca ttggtgtcgg
     6421 gatatctggt gccgaaactg gcgcgagaaa ttggcggcga acccagcaaa acgccgctgg
     6481 atctccagct acgccagcag taactgatta aacagggaag ttaaacatta gcctgatgcg
     6541 ctacgcttat caggcctaca ttgtgcattg aaattgaatg gatttgcaat gaactgtagg
     6601 ccggataagg cgtttacgcc gcatccggca agagcaacaa acgcaagtac agcaacctgt
     6661 cattaaactg ggaaatttaa tctttgcccg ttgcataacc tttaattatt catatacatt
     6721 gctgttgtta ctctttatat agccaacacc aataattaac ctttcttttc tgtttatata
     6781 gagtgagtac ggctatttat gttattttaa caactattac taccaagccc atttatctgg
     6841 cttataaatt aaaattaatt atttaattga gaaatggtta gggagaacct acatggccat
     6901 aagcatcaag ggtgttaata ccggtgttat tcgcaagagt aataatttca ttgcactggc
     6961 gctgaaaatt aaagaacctc gcaataggta atgactccaa cttattgata gtgttttatg
     7021 tt
//
LOCUS       DAJTZL010000482         4457 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.guided.242, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000482 DAJTZL010000000
VERSION     DAJTZL010000482.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4457
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.guided.242"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(1..865)
                     /gene="intI2"
                     /locus_tag="OO544_004517"
                     /pseudo
     CDS             complement(1..865)
                     /gene="intI2"
                     /locus_tag="OO544_004517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023994230.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class 2 integron integrase IntI2"
     gene            1197..1670
                     /locus_tag="OO544_004518"
     CDS             1197..1670
                     /locus_tag="OO544_004518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HCR6769904.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            1765..2289
                     /gene="sat2"
                     /locus_tag="OO544_004519"
     CDS             1765..2289
                     /gene="sat2"
                     /locus_tag="OO544_004519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HCR6769905.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            2347..3135
                     /gene="aadA1"
                     /locus_tag="OO544_004520"
     CDS             2347..3135
                     /gene="aadA1"
                     /locus_tag="OO544_004520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCR6769906.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
     gene            3211..3708
                     /locus_tag="OO544_004521"
     CDS             3211..3708
                     /locus_tag="OO544_004521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6769907.1"
                     /translation="MRDLLKVVLSLVLAMASGQALADLPIVLVDEARLPYDYSPSNYD
                     ISPSNYDNSISNYDNSPSNYDNSESNYDNSSSNYDNSRNGNRRLIYSANGSRTFAGYY
                     VIANNGTTNFFSTSGKRMFYTPKGGRGVYGGKDGSFCGALVVINGQFSLALTDNGLKI
                     MYLSN"
     gene            3769..4140
                     /locus_tag="OO544_004522"
     CDS             3769..4140
                     /locus_tag="OO544_004522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6769908.1"
                     /translation="MSYEPPRDGYTNGYIVDVMYDNRPSKYPAVFRTYVRIPDYVELD
                     NAEELADAIIREVELKEAWLTRVLGNSADPDASVGKWDRRLAQCGGIDEMSSGVQATY
                     FSPNGQLVGLVAYIDAEWMDE"
BASE COUNT         1226 a         1009 c         1097 g         1125 t
ORIGIN      
        1 gttcttgcac agtgcgaata tcacgccccg cttgtaatag atgcgtagca aacgagtgac
       61 gaaatgtatg acaagtgaca cgcttgctaa cgatgcctgc tttttgtacg gctgccttca
      121 atgcctttcg cgcaacggag tcatgcagat gatggcggca taatttgccg ttatacgggt
      181 ggttgcagag cgtgctggag ggaaagacaa acatccacgc cgcttgtcga taagcagaag
      241 ggtatttgtg atctaaagca aaaggcagcg atggccctac gccttgtaag ttgtcgtctt
      301 gctgaataag ccgcgcttgc tcaatgagtt attttattgc tgggattagg cgcgtgggca
      361 gtaggctgtt tctgcttttc ccacccttac cgtcatgcac agtgatgcag ccattatcaa
      421 aatcaaaatc tttaacccgc aaacgcaagc attcattaat gcgcaaacct gcaccataca
      481 gcagcgtaaa aataacttgg ttgcgagtat ccataacctg caaaatgcgt tgcacttcat
      541 ttgcagagat aacagagggt agccgtctag gcttgcttgc agggatataa tcaatatcgc
      601 ccaacggctg ttgtaaaaac ctgttgtaca aaaaagctag ggcatttaaa gcgattttct
      661 gcgtgtttat ggctacatgt ctgctgtttg ctaagctgga taaaaacagc ctgacctctt
      721 cactgcccat ggtctgagga tgacgttttt tgtgaaacag aataaaacgc ttaatccagt
      781 gcaggtaagt tttttcagtt ttcagcgcat aacctttttg tcgcatatcc gtgcgtatag
      841 aatttaaaaa tggactgtta gacataaaac gctccttgtc ttgcaactgt ctgcctatac
      901 agcctattct agctgggatt taaaaaagtg cctgtttttt acgcctagag atgcttgttt
      961 accggtagag ttttaattta atgctaaata aattaaaatg ttatgagttc tttgggtgag
     1021 ataatgtgca tcgtgcaagc aggatagacg gcatgcacga tttgtaataa cagagtgtct
     1081 tgtattttta aagaaagtct atttaataca agtgattata ttaattaacg gtaagcatca
     1141 gcgggtgaca aaacgagcat gcttactaat aaaatgttaa cctctgagga agaattgtga
     1201 aactatcact aatggtagct atatcgaaga atggagttat cgggaatggc cctgatattc
     1261 catggagtgc caaaggtgaa cagctcctgt ttaaagctat tacctataac caatggctgt
     1321 tggttggacg caagactttt gaatcaatgg gagcattacc caaccgaaag tatgcggtcg
     1381 taacacgttc aagttttaca tctgacaatg agaacgtatt gatctttcca tcaattaaag
     1441 atgctttaac caacctaaag aaaataacgg atcatgtcat tgtttcaggt ggtggggaga
     1501 tatacaaaag cctgatcgat caagtagata cactacatat atctacaata gacatcgagc
     1561 cggaaggtga tgtttacttt cctgaaatcc ccagcaattt taggccagtt tttacccaag
     1621 acttcgcctc taacataaat tatagttacc aaatctggca aaagggttaa caagtggcag
     1681 caacggattc gcaaacctgt cacgcctttt gtaccaaaag ccgcgccagg tttgcgatcc
     1741 gctgtgccag gcgttaggcg tcatatgaag atttcggtga tccctgagca ggtggcggaa
     1801 acattggatg ctgagaacca tttcattgtt cgtgaagtgt tcgatgtgca cctatccgac
     1861 caaggctttg aactatctac cagaagtgtg agcccctacc ggaaggatta catctcggat
     1921 gatgactctg atgaagactc tgcttgctat ggcgcattca tcgaccaaga gcttgtcggg
     1981 aagattgaac tcaactcaac atggaacgat ctagcctcta tcgaacacat tgttgtgtcg
     2041 cacacgcacc gaggcaaagg agtcgcgcac agtctcatcg aatttgcgaa aaagtgggca
     2101 ctaagcagac agctccttgg catacgatta gagacacaaa cgaacaatgt acctgcctgc
     2161 aatttgtacg caaaatgtgg ctttactctc ggcggcattg acctgttcac gtataaaact
     2221 agacctcaag tctcgaacga aacagcgatg tactggtact ggttctcggg agcacaggat
     2281 gacgcctaac aattcattca agccgacacc gcttcgcggc gcggcttaat tcaggagtta
     2341 aacatcatga gggaagcggt gatcgccgaa gtatcgactc aactatcaga ggtagttggc
     2401 gtcatcgagc gccatctcga accgacgttg ctggccgtac atttgtacgg ctccgcagtg
     2461 gatggcggcc tgaagccaca cagtgatatt gatttgctgg ttacggtgac cgtaaggctt
     2521 gatgaaacaa cgcggcgagc tttgatcaac gaccttttgg aaacttcggc ttcccctgga
     2581 gagagcgaga ttctccgcgc tgtagaagtc accattgttg tgcacgacga catcattccg
     2641 tggcgttatc cagctaagcg cgaactgcaa tttggagaat ggcagcgcaa tgacattctt
     2701 gcaggtatct tcgagccagc cacgatcgac attgatctgg ctatcttgct gacaaaagca
     2761 agagaacata gcgttgcctt ggtaggtcca gcggcggagg aactctttga tccggttcct
     2821 gaacaggatc tatttgaggc gctaaatgaa accttaacgc tatggaactc gccgcccgac
     2881 tgggctggcg atgagcgaaa tgtagtgctt acgttgtccc gcatttggta cagcgcagta
     2941 accggcaaaa tcgcgccgaa ggatgtcgct gccgactggg caatggagcg cctgccggcc
     3001 cagtatcagc ccgtcatact tgaagctagg caggcttatc ttggacaaga agatcgcttg
     3061 gcctcgcgcg cagatcagtt ggaagaattt gttcactacg tgaaaggcga gatcaccaag
     3121 gtagtcggca aataatgtct aacaattcgt tcaagccgac gccgcttcgc ggcgcggctt
     3181 aactcaagcg ttagagagct ggggaagact atgcgcgatc tgttgaaggt ggttctaagc
     3241 ctcgtacttg cgatggcatc ggggcaggca cttgctgacc tgccaattgt tttagtggat
     3301 gaagctcgtc ttccctatga ctactcccca tccaactacg acatttctcc aagcaactac
     3361 gacaactcca taagcaatta cgacaatagt ccatcaaatt acgacaactc tgagagcaac
     3421 tacgataata gttcatccaa ttacgacaat agtcgcaacg gaaatcgtag gcttatatat
     3481 agcgcaaatg ggtctcgcac tttcgccggc tactacgtca ttgccaacaa tgggacaacg
     3541 aacttctttt ccacatctgg caaaaggatg ttctacaccc caaaaggggg gcgcggcgtc
     3601 tatggcggca aagatgggag cttctgcggg gcattggtcg tcataaatgg ccaattttcg
     3661 cttgccctga cagataacgg cctgaagatc atgtatctaa gcaactagcc tgctctctaa
     3721 taaaatgtta ggcctcaaca tctagtcgca agctgagggg aaccactagt gtcatacgaa
     3781 cctccaagag acggttacac aaacgggtac attgttgatg tcatgtatga caatcgccca
     3841 agtaagtatc cagctgtgtt cagaacgtac gtccgaattc cagactatgt cgaactagat
     3901 aacgcggagg agctggccga cgcaatcatt cgagaggttg agttaaagga ggcatggctc
     3961 acaagggtct tggggaactc agccgatcct gacgcttctg tgggcaagtg ggaccggcgc
     4021 ctagcgcagt gcggtggtat tgatgagatg tcaagtgggg tgcaggcaac atacttcagt
     4081 ccaaacggtc agctggtcgg cttagtggca tacattgatg ccgaatggat ggatgaatag
     4141 ctctgacgtt acgattcagg tgagacaacc agatgggcat tgcgtacaag ctcagttgcc
     4201 accgatgtgg ctacgagtct gatcttcttt acctaggcca aggtatggcg atgcttcctg
     4261 agcaaatagt cggaacgtgc acatgctgcg acaatctaac gacgatttct ataaatgaaa
     4321 gctacggagt atgctcactt tgtggggcta aggatcgtgt gatatttaca agctatcagc
     4381 atgaggcaag gccaagaaat ccctgctatc tagagagaag tagactggcc ccctgaatct
     4441 ccagacaacc agtatca
//
LOCUS       DAJTZL010000483         4147 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466630-rid15817993.guided.255, whole genome shotgun
            sequence.
ACCESSION   DAJTZL010000483 DAJTZL010000000
VERSION     DAJTZL010000483.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466630
            Sequence Read Archive: ERR10074496
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477162.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477162.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:47:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,527
            CDSs (total)                      :: 4,455
            Genes (coding)                    :: 4,198
            CDSs (with protein)               :: 4,198
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 118 of 257
            Pseudo Genes (incomplete)         :: 67 of 257
            Pseudo Genes (internal stop)      :: 97 of 257
            Pseudo Genes (multiple problems)  :: 25 of 257
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4147
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466630-rid15817993.guided.255"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2011-01-10"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..652
                     /locus_tag="OO544_004523"
                     /pseudo
     CDS             1..652
                     /locus_tag="OO544_004523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
     gene            649..1263
                     /locus_tag="OO544_004524"
     CDS             649..1263
                     /locus_tag="OO544_004524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HCR6769909.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALERLIKEAARQDVAVTVLETET
                     GRYSDTLRSALVSLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(1320..2777)
                     /gene="pepD"
                     /locus_tag="OO544_004525"
     CDS             complement(1320..2777)
                     /gene="pepD"
                     /locus_tag="OO544_004525"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HCR6769910.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKSVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            3038..3496
                     /gene="gpt"
                     /locus_tag="OO544_004526"
     CDS             3038..3496
                     /gene="gpt"
                     /locus_tag="OO544_004526"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCR6769911.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3588..>4147
                     /locus_tag="OO544_004527"
     CDS             3588..>4147
                     /locus_tag="OO544_004527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR6769912.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     IQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLH"
BASE COUNT          999 a         1045 c         1103 g         1000 t
ORIGIN      
        1 acaccccatt ataaggggta cgaataaagt aacggggcag tctattttac agcggcatat
       61 tgcctcgttt tcgcatcatg gtttgcctga aggcagtgac gacgcgctac ggtatattgc
      121 ggaacatgac gatgcgctgg cgtttgcgcg tattaatcgc cagctgatct ctttacgcat
      181 aatgcaacag gttaaggcca ccggaagtcc ggttctggat gtggcgcata actttgttag
      241 cgcgtgtcaa atcggcgatc aacagggctg gttgcatcgt aaaggtgcca cgccggatga
      301 caacggtctg gtgattattc ccggttcact cggtgattac tcctagcttg tacagcctgt
      361 tgcgaacgaa aaaacgctcc actcgctggc gcatggggct gggcgtaaat gggggcgcac
      421 cgagtgtaaa gggcgtctgg cagcgaaata cacagcgacg cagctctcac gtactgaact
      481 tggcagtcgg gtaatttgtc gcgataaaca actcatcttt gaagaagcgc cacaagccta
      541 taaatcggct gaaagcgtgg tgcaatgtct ggtgcaggct ggtttaatta ttcctgtcgc
      601 gcggctgcgt ccggtgctaa cgctcaaaaa cagtggaggg aaaaaaggat gatcttgcta
      661 caactctcct cagcgcaggg gccggaagaa tgttgtctcg cagtgaaaaa ggcgctggag
      721 cgattaatta aagaagctgc ccgacaagac gtcgcggtaa cggtgttgga aacagaaacg
      781 ggccgctact ctgacacgct gcgttcagct ctggtttctc tggatggcga taacgcatgg
      841 gcattaagcg aaagctggtg cggcactatt cagtggattt gtccgagtcc gtatcggcct
      901 catcatgggc gcaaaaactg gtttctgggc attgggcgtt ttaccgctga tgagcaggaa
      961 caatcggatg caatccgtta tgagacgttg cgttcgtcgg ggccgggcgg tcaacatgtc
     1021 aataaaaccg actcggcggt acgcgccacg catttggcat ccggtattag cgtgaaggtt
     1081 cagtcagagc gtagtcagca tgctaacaag cggctggcac gattgctgat tgcctggaag
     1141 ctggagcagc agcaacagga aaatagcgcg gtgctgaaat cgcagcggcg aatgttccat
     1201 caccagattg aacgtggcaa cccgagacgg acatttacag ggatggcttt tatcgaagga
     1261 taatgaagga tgaaactgcc ggaaggcgat taaacgccat ccggcagcaa atcaaataat
     1321 tacttcgccg gaatttcttt cagcagttca gtcagcagtg tccagtaatg acctacgctt
     1381 tcgatgtgaa cttgctcatc cggagagtgt ggaccggtga tagttggccc gatagaaacc
     1441 atgtccattt ccggatacgg ttttttgaac agaccacatt ccaggcccgc gtggataatc
     1501 tggatgttcg gcgttttgtt gaacagacgc tgataggttt cacgtaccag atgcatcacc
     1561 ggagaattag cgtccggctg ccagccagga tatgcgcctt tcgcttcggt tttcgcgcca
     1621 gccagtttac ccagcgaatc cagcatgctc accacgtagt ctttaccgct gtcgatcaga
     1681 gaacggatca gacagtgaat ttctacgtta ttatcagtca tggtcaccac accgacgttc
     1741 agggaggttt cgaccacacc ttttgccacg tctgagttgc ggatcacacc gttcggggtg
     1801 gcgttcagca gacgaataaa ggtatcgcga gatttcgcaa tcagggcagc tttatcgttc
     1861 gctacagagt ccagcaacaa ggccagattt ttctctttct ctgccagctc gtttttcagg
     1921 atctcctgat aggtattcac cagagatttc aggacgtcga ctttatcagc tgcgacagca
     1981 atggtcgcaa aggcttcacg cgggatggcg ttacgcagtg tgccgccgtt gaaatcgata
     2041 aggcgcagat ccagttcttc tgcatgaccc gctaggaagc gcaccagcag tttgttggca
     2101 ttacccaggc caacgtggat ttccccgccg gagtgacccc ctttcagacc ttttaaggtt
     2161 aacttgaagg tttcaaaacc agctggaacc gcttcacgat ctaaatgcag gttggaggtg
     2221 aagtcgatac cccccgcaca acccatgtag atttcacctt cttcttcgga gtcggtgtta
     2281 atcagaatat cagcctgcaa ccagttgctc tgtaagccga acgcaccgtc cataccggct
     2341 tcttcggtca tggtcagcag cacttccagc gggccgtgaa ccacgttttc gtcagccaga
     2401 accgccagcg cagacgccat accaatgccg ttatctgcac ccagcgtggt gccgcgcgct
     2461 ttaacccatt cgccatcaat ataaggctgg ataggatctt tcgtgaagtc atgcacggtg
     2521 tcgttatttt tctgcggcac catatcgagg tgggcctgta agacgaccga tttacgattt
     2581 tccatacctg cggtagcagg tttacgaatc aggatattac ctacctgatc gcgttcgaca
     2641 tggaaacctt tctcttttgc ccaaccaaca atgtattcag cgagttgctc ttcatgatag
     2701 gacgggtgag gaatagaaca gattttggca aaaatatccc acagcggctg tggagataat
     2761 tgagacagtt cagacacgtt aagtctcctt gtcgatcacc cgcaaaacag tattgcaggt
     2821 cacagggtta gcagaaaatg ttgtcaacac aagacaggct tgcgagatat gtttgagaat
     2881 accactttat cccgcgtcag ggagaggcag tgcgtaaaaa gacgcggact catgtgaaat
     2941 actggttttt agtgcgccag atctctataa tctcgcgcaa cctattttcc cctcgaacac
     3001 tttttaagcc gtagataaac aggctgggac acttcacatg agcgaaaaat acatcgtcac
     3061 ctgggacatg ttgcagatcc atgcacgtaa actcgcaagc cgactgatgc cttctgaaca
     3121 atggaaaggc attattgccg taagccgtgg cggtctggta ccgggtgcgt tactggcgcg
     3181 tgaactgggt attcgtcatg tcgataccgt ttgtatttcc agctacgatc acgacaacca
     3241 gcgcgagctt aaagtgctaa aacgcgcaga aggcgatggc gaaggcttca tcgttattga
     3301 tgacctggtg gataccggtg gtactgcggt tgcgattcgt gaaatgtatc caaaagcgca
     3361 ctttgtcacc atcttcgcaa aaccggctgg tcgtccgctg gttgatgact atgttgttga
     3421 tatcccgcaa gatacctgga ttgaacagcc gtgggatatg ggcgtcgtat tcgtcccgcc
     3481 aatctccggt cgctaatctt ttcaacgcct ggcactgccg ggcgttgttc tttttaactt
     3541 caggcgggtt acaatagttt ccagtaagta ttctggaggc tgcatccatg acacaggcaa
     3601 acctgagcga aaccctgttc aaaccccgct ttaaacatcc tgaaacctcg acgctagtcc
     3661 gccgctttaa tcacggcgca caaccgcctg tgcagtcggc ccttgatggt aaaaccatcc
     3721 ctcactggta tcgcatgatt aaccgtctga tgtggatctg gcgcggcatt gacccacgtg
     3781 aaatcctcga cgtccaggcg cgtattgtga tgagcgatgc cgaacgtacc gacgatgatt
     3841 tatacgatac ggtgattggc taccgtggtg gcaactggat ttatgagtgg gccatccagg
     3901 cgatggtgtg gcaacaaaaa gcctgtgcgg aagaagatcc gcaactcagt ggtcgtcact
     3961 ggctgcatgc ggctacgttg tacaacattg ctgcctatcc tcatctgaaa ggagatgacc
     4021 tggccgagca agcgcaggct ttgtcaaacc gtgcctatga agaagccgcc cagcgtctgc
     4081 cgggcacgat gcggcagatg gagtttaccg tacccggcgg tgcgcccatc accggctttt
     4141 tgcatat
//
